HTR-TN achievements and prospects for future developments

International Nuclear Information System (INIS)

Hittner, D.; Angulo, C.; Basini, V.; Bogusch, E.; Breuil, E.; Buckthorpe, D.; Chauvet, V.; Futterer, M.A.; Van Heek, A.; Von Lensa, W.; Yvon, P.

2011-01-01

It is already 10 years since the (European) High Temperature Reactor Technology Network (HTR-TN) launched a program for development of HTR technology, which expanded through three successive Euratom framework programs, with many projects in line with the network strategy. Widely relying in the beginning on the legacy of the former European HTR developments (DRAGON, AVR, THTR, etc.) that it contributed to safeguard, this program led to advances in HTR/VHTR technologies and produced significant results, which can contribute to the international cooperation through Euratom involvement in the Generation IV International Forum (GIF). the main achievements of the European program, performed in complement to efforts made in several European countries and other GIF partners, are presented: they concern the validation of computer codes (reactor physics, as well as system transient analysis from normal operation to air ingress accident and fuel performance in normal and accident conditions), materials (metallic materials for vessel, direct cycle turbines and intermediate heat exchanger, graphite, etc.), component development, fuel manufacturing and irradiation behavior, and specific HTR waste management (fuel and graphite). Key experiments have been performed or are still ongoing, like irradiation of graphite and of fuel material (PYCASSO experiment), high burn-up fuel PIE, safety test and isotopic analysis, IHX mock-up thermohydraulic test in helium atmosphere, air ingress experiment for a block type core, etc. Now HTR-TN partners consider that it is time for Europe to go a step forward toward industrial demonstration. In line with the orientations of the 'Strategic Energy Technology Plan (SET-Plan)' recently issued by the European Commission that promotes a strategy for development of low-carbon energy technologies and mentions Generation IV nuclear systems as part of key technologies, HTR-TN proposes to launch a program for extending the contribution of nuclear energy to

Rust Contamination from Water Leaks in the Cosmic Dust Lab and Lunar and Meteorite Thin Sections Labs at Johnson Space Center

Science.gov (United States)

Kent, J. J.; Berger, E. L.; Fries, M. D.; Bastien, R.; McCubbin, F. M.; Pace, L.; Righter, K.; Sutter, B.; Zeigler, R. A.; Zolensky, M.

2017-01-01

On the early morning of September 15th, 2016, on the first floor of Building 31 at NASA-Johnson Space Center, the hose from a water chiller ruptured and began spraying water onto the floor. The water had been circulating though old metal pipes, and the leaked water contained rust-colored particulates. The water flooded much of the western wing of the building's ground floor before the leak was stopped, and it left behind a residue of rust across the floor, most notably in the Apollo and Meteorite Thin Section Labs and Sample Preparation Lab. No samples were damaged in the event, and the affected facilities are in the process of remediation. At the beginning of 2016, a separate leak occurred in the Cosmic Dust Lab, located in the same building. In that lab, a water leak occurred at the bottom of the sink used to clean the lab's tools and containers with ultra-pure water. Over years of use, the ultra-pure water eroded the metal sink piping and leaked water onto the inside of the lab's flow bench. This water also left behind a film of rusty material. The material was cleaned up and the metal piping was replaced with PVC pipe and sealed with Teflon plumber's tape. Samples of the rust detritus were collected from both incidents. These samples were imaged and analyzed to determine their chemical and mineralogical compositions. The purpose of these analyses is to document the nature of the detritus for future reference in the unlikely event that these materials occur as contaminants in the Cosmic Dust samples or Apollo or Meteorite thin sections.

Influence of local regulations on TN dual purpose BWR casks

International Nuclear Information System (INIS)

Samson, P.; Neider, T.

1999-01-01

Transnucleaire (Paris, France) and Transnuclear, Inc. (Hawthorne, New York, United Sates) have both developed Pressurized Water Reactor (PWR) spent fuel casks for storage and transport purposes. The products are supplied in Europe by Transnucleaire and in the United States by Transnuclear, Inc. Now the TN Group is working on a design for Boiling Water Reactor (BWR) spent fuel assemblies: the TN 52 L cask is designed for transport and storage is Switzerland, the TN 68 cask is designed for transport and storage in the United States. For storage purpose, national regulatory requirements have to be met: each country has specific demands and criteria. As a consequence, differences between the TN 52 L design and the TN 68 design for rather similar contents appear in several fields: the design work, the licensing process, the manufacturing and the operational life. (author)

Prediction of Microcystis Blooms Based on TN:TP Ratio and Lake Origin

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Yoshimasa Amano

2008-01-01

Full Text Available We evaluated the relationship between TN:TP ratio and Microcystis growth via a database that includes worldwide lakes based on four types of lake origin (dammed, tectonic, coastal, and volcanic lakes. We used microcosm and mesocosm for the nutrient elution tests with lake water and four kinds of sediment (nontreated, MgO sprinkling treated, dissolved air flotation [DAF] treated, and combined treated sediment in order to control TN:TP ratio and to suppress Microcystis growth. Microcystis growth was related to TN:TP ratio, with the maximum value at an optimum TN:TP ratio and the minimum values when the TN:TP ratios reached to 0 or ∞. The kurtosis of the distribution curve varied with the type of lake origin; the lowest kurtosis was found in dammed lakes, while the highest was found in volcanic lakes. The lake trophic state could affect the change in the kurtosis, providing much lower kurtosis at eutrophic lakes (dammed lakes than that at oligotrophic lakes (volcanic lakes. The relationship between TN:TP ratio and Microcystis growth could be explained by the nutrient elution tests under controlled TN:TP ratios through the various sediment treatments. A significant suppression of Microcystis growth of 70% could be achieved when the TN:TP ratios exceeded 21. Lake origin could be regarded as an index including morphological and geographical factors, and controlling the trophic state in lakes. The origin rather than trophic state for lakes could be considered as an important factor of TN:TP influences on Microcystis growth.

Comparative Analysis of Stress Induced Gene Expression in Caenorhabditis elegans following Exposure to Environmental and Lab Reconstituted Complex Metal Mixture.

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Ranjeet Kumar

Full Text Available Metals are essential for many physiological processes and are ubiquitously present in the environment. However, high metal concentrations can be harmful to organisms and lead to physiological stress and diseases. The accumulation of transition metals in the environment due to either natural processes or anthropogenic activities such as mining results in the contamination of water and soil environments. The present study used Caenorhabditis elegans to evaluate gene expression as an indicator of physiological response, following exposure to water collected from three different locations downstream of a Swedish mining site and a lab reconstituted metal mixture. Our results indicated that the reconstituted metal mixture exerted a direct stress response in C. elegans whereas the environmental waters elicited either a diminished or abrogated response. This suggests that it is not sufficient to use the biological effects observed from laboratory mixtures to extrapolate the effects observed in complex aquatic environments and apply this to risk assessment and intervention.

On-line liquid phase micro-extraction based on drop-in-plug sequential injection lab-at-valve platform for metal determination

Energy Technology Data Exchange (ETDEWEB)

Mitani, Constantina [Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University, Thessaloniki 54124 (Greece); Anthemidis, Aristidis N., E-mail: anthemid@chem.auth.gr [Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University, Thessaloniki 54124 (Greece)

2013-04-10

Highlights: ► Drop-in-plug micro-extraction based on SI-LAV platform for metal preconcentration. ► Automatic liquid phase micro-extraction coupled with FAAS. ► Organic solvents with density higher than water are used. ► Lead determination in environmental water and urine samples. -- Abstract: A novel automatic on-line liquid phase micro-extraction method based on drop-in-plug sequential injection lab-at-valve (LAV) platform was proposed for metal preconcentration and determination. A flow-through micro-extraction chamber mounted at the selection valve was adopted without the need of sophisticated lab-on-valve components. Coupled to flame atomic absorption spectrometry (FAAS), the potential of this lab-at-valve scheme is demonstrated for trace lead determination in environmental and biological water samples. A hydrophobic complex of lead with ammonium pyrrolidine dithiocarbamate (APDC) was formed on-line and subsequently extracted into an 80 μL plug of chloroform. The extraction procedure was performed by forming micro-droplets of aqueous phase into the plug of the extractant. All critical parameters that affect the efficiency of the system were studied and optimized. The proposed method offered good performance characteristics and high preconcentration ratios. For 10 mL sample consumption an enhancement factor of 125 was obtained. The detection limit was 1.8 μg L{sup −1} and the precision expressed as relative standard deviation (RSD) at 50.0 μg L{sup −1} of lead was 2.9%. The proposed method was evaluated by analyzing certified reference materials and applied for lead determination in natural waters and urine samples.

Advanced LabVIEW Labs

International Nuclear Information System (INIS)

Jones, Eric D.

1999-01-01

In the world of computer-based data acquisition and control, the graphical interface program LabVIEW from National Instruments is so ubiquitous that in many ways it has almost become the laboratory standard. To date, there have been approximately fifteen books concerning LabVIEW, but Professor Essick's treatise takes on a completely different tack than all of the previous discussions. In the more standard treatments of the ways and wherefores of LabVIEW such as LabVIEW Graphical Programming: Practical Applications in Instrumentation and Control by Gary W. Johnson (McGraw Hill, NY 1997), the emphasis has been instructing the reader how to program LabVIEW to create a Virtual Instrument (VI) on the computer for interfacing to a particular instruments. LabVIEW is written in ''G'' a graphical programming language developed by National Instruments. In the past the emphasis has been on training the experimenter to learn ''G''. Without going into details here, ''G'' incorporates the usual loops, arithmetic expressions, etc., found in many programming languages, but in an icon (graphical) environment. The net result being that LabVIEW contains all of the standard methods needed for interfacing to instruments, data acquisition, data analysis, graphics, and also methodology to incorporate programs written in other languages into LabVIEW. Historically, according to Professor Essick, he developed a series of experiments for an upper division laboratory course for computer-based instrumentation. His observation was that while many students had the necessary background in computer programming languages, there were students who had virtually no concept about writing a computer program let alone a computer- based interfacing program. Thus the beginnings of a concept for not only teaching computer- based instrumentation techniques, but aiso a method for the beginner to experience writing a com- puter program. Professor Essick saw LabVIEW as the ''perfect environment in which to

An insight into the thermodynamic characteristics of human thrombopoietin complexation with TN1 antibody

Science.gov (United States)

Shibazaki, Chie; Adachi, Motoyasu; Honjo, Eijiro; Tamada, Taro; Maeda, Yoshitake; Tahara, Tomoyuki; Kato, Takashi; Miyazaki, Hiroshi; Blaber, Michael; Kuroki, Ryota

2016-01-01

Abstract Human thrombopoietin (hTPO) primarily stimulates megakaryocytopoiesis and platelet production and is neutralized by the mouse TN1 antibody. The thermodynamic characteristics of TN1 antibody–hTPO complexation were analyzed by isothermal titration calorimetry (ITC) using an antigen‐binding fragment (Fab) derived from the TN1 antibody (TN1‐Fab). To clarify the mechanism by which hTPO is recognized by TN1‐Fab the conformation of free TN1‐Fab was determined to a resolution of 2.0 Å using X‐ray crystallography and compared with the hTPO‐bound form of TN1‐Fab determined by a previous study. This structural comparison revealed that the conformation of TN1‐Fab does not substantially change after hTPO binding and a set of 15 water molecules is released from the antigen‐binding site (paratope) of TN1‐Fab upon hTPO complexation. Interestingly, the heat capacity change (ΔCp) measured by ITC (−1.52 ± 0.05 kJ mol−1 K−1) differed significantly from calculations based upon the X‐ray structure data of the hTPO‐bound and unbound forms of TN1‐Fab (−1.02 ∼ 0.25 kJ mol−1 K−1) suggesting that hTPO undergoes an induced‐fit conformational change combined with significant desolvation upon TN1‐Fab binding. The results shed light on the structural biology associated with neutralizing antibody recognition. PMID:27419667

Establishment of colloid gold immunity chromatography assay for cardiac troponin I (cTnI)

International Nuclear Information System (INIS)

Wang Dezhi; Chen Jiying; Qin Lili; Zhao Baojian; Zhang Chunming

2006-01-01

Objective: To establish the colloid gold Immunity chromatography assay for cardiac troponin I. Methods: To purify cTnI from human cardiac muscle and immunize rabbit with it. cTnI antibody of rabbit anti-human cardiac muscle has been prepared and colloid gold immunity chromatography assay was established by using immunity chromatography technology. Results: Anti-serum titles of cTnI were 1:100000, Ka=2.38 x 10 9 L/mol; Methodological index: Sensitivity: 5 ng/ml; Specificity: cTnI is no cross-reaction with cTnT, cTnC and CK-MB. conclusion: The assay is highly specific, quick and simple. It can be widely used for the early diagnosis of AMI and scientific research. (authors)

ST6GalNAc-I controls expression of sialyl-Tn antigen in gastrointestinal tissues

DEFF Research Database (Denmark)

Marcos, Nuno T; Bennett, Eric P; Gomes, Joana

2011-01-01

Sialyl-Tn is a simple mucin-type carbohydrate antigen aberrantly expressed in gastrointestinal adenocarcinomas and in the precursor lesion intestinal metaplasia. Sialyl-Tn tumour expression is an independent indicator of poor prognosis. We have previously shown in vitro that ST6GalNAc-I and ST6GalNAc......-II sialyltransferases can synthesize sialyl-Tn. The aim of the present study was to establish whether ST6GalNAc-I is the major enzyme responsible for the expression of sialyl-Tn. We used a model of CHO-ldlD cells producing only MUC1-Tn glycoform and showed that ST6GalNAc-I is the key-enzyme leading to sialyl......-Tn biosynthesis. We developed novel monoclonal antibodies specific for ST6GalNAc-I and evaluated its expression in gastrointestinal tissues. ST6GalNAc-I was detected in normal colon mucosa co-localized with O-acetylated sialyl-Tn. Expression was largely unaltered in colorectal adenocarcinomas. In contrast, we...

Lab-based x-ray tomography of a cochlear implant using energy discriminating detectors for metal artefact reduction

Science.gov (United States)

Yokhana, Viona S. K.; Arhatari, Benedicta D.; Gureyev, Timur E.; Abbey, Brian

2018-01-01

X-ray computed tomography (XCT) is an important clinical diagnostic tool which is also used in a range of biological imaging applications in research. The increasing prevalence of metallic implants in medical and dental radiography and tomography has driven the demand for new approaches to solving the issue of metal artefacts in XCT. Metal artefacts occur when a highly absorbing material is imaged which is in boundary contact with one or more weakly absorbing components, such as soft-tissue. The resulting `streaking' in the reconstructed images creates significant challenges for X-ray analysis due to the non-linear dependence on the absorption properties of the sample. In this paper we introduce a new approach to removing metal artefacts which exploits the capabilities of the recently available, photon-counting PiXirad detector. Our approach works for standard lab-based polychromatic X-ray tubes and does not rely on any postprocessing of the data. The method is demonstrated using both simulated data from a test phantom and experimental data collected from a cochlear implant. The results show that by combining the individual images, which are simultaneously generated for each different energy threshold, artefact -free segmentation of the implant from the surrounding biological tissue is achieved.

Myofilament Calcium Sensitivity: Mechanistic Insight into TnI Ser-23/24 and Ser-150 Phosphorylation Integration

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Hussam E Salhi

2016-12-01

Full Text Available Troponin I (TnI is a major regulator of cardiac muscle contraction and relaxation. During physiological and pathological stress, TnI is differentially phosphorylated at multiple residues through different signaling pathways to match cardiac function to demand. The combination of these TnI phosphorylations can exhibit an expected or unexpected functional integration, whereby the function of two phosphorylations are different than that predicted from the combined function of each individual phosphorylation alone. We have shown that TnI Ser-23/24 and Ser-150 phosphorylation exhibit functional integration and are simultaneously increased in response to cardiac stress. In the current study, we investigated the functional integration of TnI Ser-23/24 and Ser-150 to alter cardiac contraction. We hypothesized that Ser-23/24 and Ser-150 phosphorylation each utilize distinct molecular mechanisms to alter the TnI binding affinity within the thin filament. Mathematical modeling predicts that Ser-23/24 and Ser-150 phosphorylation affect different TnI affinities within the thin filament to distinctly alter the Ca2+-binding properties of troponin. Protein binding experiments validate this assertion by demonstrating pseudo-phosphorylated Ser-150 decreases the affinity of isolated TnI for actin, whereas Ser-23/24 pseudo-phosphorylation is not different from unphosphorylated. Thus, our data supports that TnI Ser-23/24 affects TnI-TnC binding, while Ser-150 phosphorylation alters TnI-actin binding. By measuring force development in troponin-exchanged skinned myocytes, we demonstrate that the Ca2+ sensitivity of force is directly related to the amount of phosphate present on TnI. Furthermore, we demonstrate that Ser-150 pseudo-phosphorylation blunts Ser-23/24-mediated decreased Ca2+-sensitive force development whether on the same or different TnI molecule. Therefore, TnI phosphorylations can integrate across troponins along the myofilament. These data demonstrate

Antibiotic and antiseptic resistance genes are linked on a novel mobile genetic element: Tn6087

Science.gov (United States)

Ciric, Lena; Mullany, Peter; Roberts, Adam P.

2011-01-01

Objectives Tn916-like elements are one of the most common types of integrative and conjugative element (ICE). In this study we aimed to determine whether novel accessory genes, i.e. genes whose products are not involved in mobility or regulation, were present on a Tn916-like element (Tn6087) isolated from Streptococcus oralis from the human oral cavity. Methods A minocycline-resistant isolate was analysed using restriction fragment length polymorphism (RFLP) analysis on amplicons derived from Tn916 and DNA sequencing to determine whether there were genetic differences in Tn6087 compared with Tn916. Mutational analysis was used to determine whether the novel accessory gene found was responsible for an observed extra phenotype. Results A novel Tn916-like element, Tn6087, is described that encodes both antibiotic and antiseptic resistance. The antiseptic resistance protein is encoded by a novel small multidrug resistance gene, designated qrg, that was shown to encode resistance to cetyltrimethylammonium bromide (CTAB), also known as cetrimide bromide. Conclusions This is the first Tn916-like element described that confers both antibiotic and antiseptic resistance, suggesting that selection of either antibiotic or antiseptic resistance will also select for the other and further highlights the need for prudent use of both types of compound. PMID:21816764

Graphene Oxide-Poly(dimethylsiloxane)-Based Lab-on-a-Chip Platform for Heavy-Metals Preconcentration and Electrochemical Detection.

Science.gov (United States)

Chałupniak, Andrzej; Merkoçi, Arben

2017-12-27

Herein, we present the application of a novel graphene oxide-poly(dimethylsiloxane) (GO-PDMS) composite in reversible adsorption/desorption, including detection of heavy metals. GO-PDMS was fabricated by simple blending of GO with silicon monomer in the presence of tetrahydrofuran, followed by polymerization initiated upon the addition of curing agent. We found GO concentration, curing agent concentration, pH, and contact time among the most important factors affecting the adsorption of Pb(II) used as a model heavy metal. The mechanism of adsorption is based on surface complexation, where oxygen active groups of negative charge can bind with bivalent metal ions Me(II). To demonstrate a practical application of this material, we fabricated microfluidic lab-on-a-chip platform for heavy-metals preconcentration and detection. This device consists of a screen-printed carbon electrode, a PDMS chip, and a GO-PDMS chip. The use of GO-PDMS preconcentration platform significantly improves the sensitivity of electrochemical detection of heavy metals (an increase of current up to 30× was observed), without the need of modifying electrodes or special reagents addition. Therefore, samples being so far below the limit of detection (0.5 ppb) were successfully detected. This approach is compatible also with real samples (seawater) as ionic strength was found as indifferent for the adsorption process. To the best of our knowledge, GO-PDMS was used for the first time in sensing application. Moreover, due to mechanical resistance and outstanding durability, it can be used multiple times unlike other GO-based platforms for heavy-metals adsorption.

Tennessee Comptroller of the Treasury (TN COMP)

Data.gov (United States)

Social Security Administration — The Tennessee Comptroller of the Treasury (TN COMP) uses SSA's assistance in administering the Tennessee Property Tax Rebate Program. SSA provides assistance through...

A New Generalizable Test for Detection of Mutations Affecting Tn10 Transposition

OpenAIRE

Huisman, Olivier; Kleckner, Nancy

1987-01-01

We describe here a new rapid screen that allows easy detection of transposon or host mutations that affect Tn10 transposition in Escherichia coli. This test involves a new Tn10 derivative called the "mini-lacZ-kanR fusion hopper" or mini-Tn10-LK for short. This element does not direct expression of β-galactosidase when present at its original starting location on a suitably engineered plasmid or phage genome because it lacks appropriate transcription and translation start signals. However, t...

Variation on a theme; an overview of the Tn916 / Tn1545 family of mobile genetic elements in the oral and nasopharyngeal streptococci.

Directory of Open Access Journals (Sweden)

Francesco eSantoro

2014-10-01

Full Text Available The oral and nasopharyngeal streptococci are a major part of the normal microbiota in humans. Most human associated streptococci are considered commensals however a small number of them are pathogenic, causing a wide range of diseases including oral infections such as dental caries and periodontitis and diseases at other body sites including sinusitis and endocarditis, and in the case of Streptococcus pneumoniae, meningitis. Both phenotypic and sequence based studies have shown that the human associated streptococci from the mouth and nasopharynx harbour a large number of antibiotic resistance genes and these are often located on mobile genetic elements known as conjugative transposons or integrative and conjugative elements of the Tn916 / Tn1545 family. These mobile genetic elements are responsible for the spread of the resistance genes between streptococci and also between streptococci and other bacteria. In this review we describe the resistances conferred by, and the genetic variations between the many different Tn916-like elements found in recent studies of oral and nasopharyngeal streptococci and show that Tn916-like elements are important mediators of antibiotic resistance genes within this genus. We will also discuss the role of the oral environment and how this is conducive to the transfer of these elements and discuss the contribution of both transformation and conjugation on the transfer and evolution of these elements in different streptococci.

ETIOLOGY CLASSIFICATION AND TREATMENT NEEDS (TN FOR ORAL MALODOR

Directory of Open Access Journals (Sweden)

Anton Raharjo

2015-08-01

Full Text Available Background: Oral malodor, a generic descriptor term for foul smells emanating from the mouth can be classified as either pathological or physiological halitosis. Some problems are often confounded by the clinician's mismanagement. Objective: This paper reviews the etiology of classification and determination of treatment needs (TN for oral malodor. Literature review and discussion: In the majority of cases the problem has been shown to originate in the oral cavity. Although oral malodor cases are often related to physiological aspects, sometimes they can be related to extra oral sources and psychological aspects. Classification methods of oral malodor with corresponding treatment needs (TN have already been established. Although PTC & tongue brushing and appropriate mouthrinses are both important and basic treatment measures for halitosis, other dental treatments are sometimes required. Conclusion: Accurate screening and diagnosis of halitosis followed by appropriate TN may give better results and consequently reduce the risk of mismanagement.

A Universal Approach for Selective Trace Metal Determinations via Sequential Injection-Bead Injection-Lab-on-Valve (SI-BI-LOV) Using Renewable Reagent-loaded Hydrophobic Beads

DEFF Research Database (Denmark)

Long, Xiangbao; Miró, Manuel; Hansen, Elo Harald

-Lab-on-Valve (SI-LOV) mode. The methodology uses poly(styrene-divinylbenzene) beads containing pendant octadecyl moieties (C18-PS/DVB), which are pre-impregnated with a selective organic metal chelating agent prior to the automatic manipulation of the beads in the microbore conduits of the LOV unit. By adapting...

In-site interaction evaluation of Tn density by inhibition/competition assays

Energy Technology Data Exchange (ETDEWEB)

Robles, Ana [Radiopharmacy Department, Nuclear Research Center, Faculty of Sciences, University of the Republic, Montevideo (Uruguay)], E-mail: anamar@cin.edu.uy; Medeiros, Andrea [Biochemistry Department, Faculty of Medicine, University of the Republic, Montevideo (Uruguay); Berois, Nora [Laboratory of Glycobiology and Tumor Immunology, Pasteur Institute of Montevideo (Uruguay); Balter, Henia S. [Radiopharmacy Department, Nuclear Research Center, Faculty of Sciences, University of the Republic, Montevideo (Uruguay); Pauwels, Ernest K. [University Medical Center Leiden, Department of Radiology, Leiden (Netherlands); Osinaga, Eduardo [Laboratory of Glycobiology and Tumor Immunology, Pasteur Institute of Montevideo (Uruguay); Department of Immunobiology, Faculty of Medicine, University of the Republic, Montevideo (Uruguay)

2010-05-15

The tumor-associated structure N-acetyl-galactosamine-O-Ser/Thr (Tn antigen), which is overexpressed in various tumor cell types, notably of the breast, ovary and colon, is an interesting determinant that is useful for cancer diagnosis and follow-up. The aim of this research was to study different assay strategies in order to determine the most sensitive system for further application in epitope characterization and binding assessment. The tetrameric isolectin obtained from Vicia villosa seeds (VVLB{sub 4}) shows high affinity for the tumor-associated structure. A monoclonal antibody against VVLB{sub 4}, MabVV{sub 34}, was generated, and the interaction between MabVV{sub 34} and VVLB{sub 4} was studied by means of binding and inhibition assays. Several synthetic peptides (10 amino acid sequences) designed from the amino acid sequence of VVLB{sub 4} and obtained from trypsin digestion were tested to determine which amino acids were involved in the interaction between MabVV{sub 34} and VVLB{sub 4}. The further unraveling of this epitope was investigated by inhibition using designed synthetic peptides as well as mixtures mimicking variable density effect. Under the experimental circumstances, MabVV{sub 34} was able to inhibit the binding of VVLB{sub 4} to Tn. Two of the four peptide sequences assayed showed better inhibition properties. Finally, mixtures containing these selected sequences allowed the evaluation of binding and inhibition as a function of Tn density. We conclude that the present study facilitates the further development of a specific Tn marker and may contribute to the development of Tn-like radiolabelled peptides or Tn-specific radiolabelled fragments providing a highly selective tool for cancer diagnosis and treatment. This strategy may contribute to characterize the new generation of radiopharmaceuticals for diagnosis and therapy based on biomolecules like antibodies, fragments or peptides, whose application is directly guided by their specific

Some reproductive performances of Thai native (TN and 50% TN x Anglo-Nubian crossbred does with different levels of concentrate supplementation

Directory of Open Access Journals (Sweden)

Choldumrongkul, S.

2007-05-01

Full Text Available A 2 x 2 x 2 factorial in completely randomized design was conducted to determine the effect of genotype of does (Thai native (TN or 50% Anglo-Nubian (AN crossbred, levels of concentrate supplementation(1 % body weight or ad libitum and body condition of does (poor or good on oestrus incidence, kidding rate and multiple birth rate of does. Does rotationally grazed on Paspalum plicatulum pasture for 4 weeks andwere supplemented with concentrate. The experiment was divided into 4 periods: 1 does run with a teaser for 105 days before mating to determine an oestrus incidence 2 does joined with bucks of the same genotypefor 45 days 3 does run with a teaser for 150 days during pregnancy and 4 does run with a teaser 90 days post-partum. Genotype, levels of concentrate supplementation and body condition of does did not significantlyaffect (P>0.05 kidding rate or multiple birth rate and average kidding rate and multiple birth rate of the does was 83.8 and 82.3%, respectively. Birth weight of kids from TN does was significantly lower (P<0.01 than that from 50% TN x AN crossbred does (1.9 and 2.5 kg, respectively. Number of days of the first oestrus incidence in post-partum period for TN does was significantly (P<0.01 less than that of 50% ANcrossbred does (59.1 days and 75.0 days, respectively. Number of days of the first oestrus incidence in postpartum for does supplemented with concentrate ad libitum was significantly (P<0.01 lower than that ofdoes supplemented with 1% BW of concentrate (60.5 and 73.6 days, respectively.

ST6GalNAc-I controls expression of sialyl-Tn antigen in gastrointestinal tissues

DEFF Research Database (Denmark)

Marcos, Nuno T; Bennett, Eric Paul; Gomes, Joana

2011-01-01

-Tn biosynthesis. We developed novel monoclonal antibodies specific for ST6GalNAc-I and evaluated its expression in gastrointestinal tissues. ST6GalNAc-I was detected in normal colon mucosa co-localized with O-acetylated sialyl-Tn. Expression was largely unaltered in colorectal adenocarcinomas. In contrast, we......NAc-I as the major enzyme controlling the expression of cancer-associated sialyl-Tn antigen in gastrointestinal tissues....

cTnT can be a useful marker for early detection of anthracycline cardiotoxicity.

Science.gov (United States)

Kilickap, S; Barista, I; Akgul, E; Aytemir, K; Aksoyek, S; Aksoy, S; Celik, I; Kes, S; Tekuzman, G

2005-05-01

The level of serum cardiac troponin-T (cTnT) increases with myocardial damage. We sought to assess whether cTnT level could be a useful marker for the early detection of anthracycline cardiotoxicity. Forty-one patients who had been scheduled to receive anthracycline-containing combination chemotherapy were included in the study. Serum cTnT levels were measured before (baseline) and after the first cycle of chemotherapy, and again, after the last cycle of chemotherapy. In all patients, the left ventricular ejection fraction (LVEF), fractional shortening (FS), early peak flow/atrial flow velocity (E/A) ratio, and the isovolemic relaxation time (IRT) were measured echocardiographically, both before and after the completion of chemotherapy. LVEF and FS did not change in any patients. In 21 patients (49%), the E/A ratio decreased after therapy as compared to the pre-treatment values. The decrease in E/A ratio was more prominent in patients who were older than the mean age of our study group, which was 44 years. The post-treatment IRT was prolonged compared with the pretreatment IRT (94.0 +/- 2.0 versus 85.6 +/- 10.5 ms, respectively). cTnT levels after completion of therapy were elevated in 14 (34%) patients, and exceeded the upper limit of the normal range (>0.1 ng/ml) in only one patient. cTnT levels measured after completion of therapy were significantly higher, compared with those measured at baseline and after the first cycle of therapy. In the younger age group (< or =44 years old), there was a two-fold decrease in the E/A ratio in those patients whose cTnT levels increased during the therapy, when compared with those whose cTnT levels did not change (21% versus 43%, respectively). Increased serum cTnT level can be detected in the early stages of anthracycline therapy and it is associated with diastolic dysfunction of the left ventricle. Therefore, serum cTnT level could be a useful measure for early detection of anthracycline-induced cardiotoxicity.

Induction of transposon TN1 translocation under the action of different mutagens

International Nuclear Information System (INIS)

Kubanejshvili, M.G.; Smirnov, S.P.; Tarasov, V.A.

1983-01-01

Migration of ampicillin transposon Tn1 under normal conditions in Escherichia coli cells proceeds with low frequency (10 -4 transpositions for cell). The low transposition frequency is conditioned by the transposition repression, realized by the gene-repressor in transposon structure and, probably, by other regulating genes of the bacterium-host. E. coli cell treatment by physical and chemical mutagens resulted in induction of translocation of ampicillin transposon Tn1 from plasmid RP4 into other replicons. Mitomycin C and ultraviolet radiation produced stronger inducing effect as compared to nitroso-guanidine (NG). The effect of the given mutagens on transposition Tn1 correlated with their activating capacity with respect to inducible SOS-functions of E coli. The mutation of rec A didn't influence on spontaneous Tn1 transposition, but blocked completely the induction of transposition process under mutagen effect. The relationship of inducible transposition with SOS-functions in E. coli cells, controlled by recA and lexA genes, as well as the possible role of the process in genetic microorganism variability are discussed in the paper

Comblike dendrimers containing Tn antigen modulate natural killing and induce the production of Tn specific antibodies

Czech Academy of Sciences Publication Activity Database

Vepřek, Pavel; Hajdúch, M.; Džubák, P.; Kulík, R.; Poláková, J.; Bezouška, Karel

2006-01-01

Roč. 49, č. 21 (2006), s. 6400-6407 ISSN 0022-2623 R&D Projects: GA AV ČR IAA4020213; GA AV ČR IAA5020403; GA ČR GA304/06/1691 Institutional research plan: CEZ:AV0Z40550506; CEZ:AV0Z50200510 Keywords : Tn antigen * glycodendrimer * NKP-P1 Subject RIV: CE - Biochemistry Impact factor: 5.115, year: 2006

Two-stage soil infiltration treatment system for treating ammonium wastewaters of low COD/TN ratios.

Science.gov (United States)

Lei, Zhongfang; Wu, Ting; Zhang, Yi; Liu, Xiang; Wan, Chunli; Lee, Duu-Jong; Tay, Joo-Hwa

2013-01-01

Soil infiltration treatment (SIT) is ineffective to treat ammonium wastewaters of total nitrogen (TN) > 100 mg l(-1). This study applied a novel two-stage SIT process for effective TN removal from wastewaters of TN>100 mg l(-1) and of chemical oxygen demand (COD)/TN ratio of 3.2-8.6. The wastewater was first fed into the soil column (stage 1) at hydraulic loading rate (HLR) of 0.06 m(3) m(-2) d(-1) for COD removal and total phosphorus (TP) immobilization. Then the effluent from stage 1 was fed individually into four soil columns (stage 2) at 0.02 m(3) m(-2) d(-1) of HLR with different proportions of raw wastewater as additional carbon source. Over the one-year field test, balanced nitrification and denitrification in the two-stage SIT revealed excellent TN removal (>90%) from the tested wastewaters. Copyright © 2012 Elsevier Ltd. All rights reserved.

Guidelines for the segregation characterization management of dry waste at Berkeley Lab

International Nuclear Information System (INIS)

1997-05-01

Managing and disposing of dry low level radioactive waste at Berkeley Lab. is problematic. The Waste Management Group must assure off site treatment, storage, and disposal facilities that dry waste from Berkeley Lab. is free of liquids and regulated metals (such as lead and mercury). RTR (Real Time Radioagraphy) used for waste to be rejected. This pamphlet helps to clarify dry waste management requirements that will ensure that Berkeley Lab. dry waste will be accepted for off site shipment. These issues are critical if we are to have an off site disposal option for your dry radioactive waste

76 FR 14855 - Television Broadcasting Services; Nashville, TN

Science.gov (United States)

2011-03-18

... FEDERAL COMMUNICATIONS COMMISSION 47 CFR Part 73 [MB Docket No. 11-29, RM-11622; DA 11-335] Television Broadcasting Services; Nashville, TN AGENCY: Federal Communications Commission. ACTION: Proposed... 73 Television, Television broadcasting. Federal Communications Commission. Kevin R. Harding...

76 FR 33656 - Television Broadcasting Services; Nashville, TN

Science.gov (United States)

2011-06-09

... FEDERAL COMMUNICATIONS COMMISSION 47 CFR Part 73 [MB Docket No. 11-29; RM-11622, DA 11-949] Television Broadcasting Services; Nashville, TN AGENCY: Federal Communications Commission. ACTION: Final rule... Part 73 Television. Federal Communications Commission. Barbara A. Kreisman, Chief, Video Division...

Bovine Serum Albumin Metal Complexes for Mimic of SOD

Indian Academy of Sciences (India)

Key Lab. Eco-Environment-Related Polymer Materials of Ministry of Education, Key Lab. ... scaffold and the metal complex functioned as the catalytic active center. ... small molecule.22 It is found that the antioxidative ... and absence, respectively, of the measured compound. ... monitor the interaction of metal ions with BSA.

An Enterobacter plasmid as a new genetic background for the transposon Tn1331

Directory of Open Access Journals (Sweden)

Alavi MR

2011-11-01

Full Text Available Mohammad R Alavi1,2, Vlado Antonic2, Adrien Ravizee1, Peter J Weina3, Mina Izadjoo1,2, Alexander Stojadinovic21Division of Wound Biology and Translational Research, Armed Forces Institute of Pathology and American Registry of Pathology, Washington DC, 2Combat Wound Initiative Program, Walter Reed Army Medical Center, Washington DC, 3The Walter Reed Army Institute of Research, Silver Spring, MD, USABackground: Genus Enterobacter includes important opportunistic nosocomial pathogens that could infect complex wounds. The presence of antibiotic resistance genes in these microorganisms represents a challenging clinical problem in the treatment of these wounds. In the authors’ screening of antibiotic-resistant bacteria from complex wounds, an Enterobacter species was isolated that harbors antibiotic-resistant plasmids conferring resistance to Escherichia coli. The aim of this study was to identify the resistance genes carried by one of these plasmids.Methods: The plasmids from the Enterobacter isolate were propagated in E. coli and one of the plasmids, designated as pR23, was sequenced by the Sanger method using fluorescent dye-terminator chemistry on a genetic analyzer. The assembled sequence was annotated by search of the GenBank database.Results: Plasmid pR23 is composed of the transposon Tn1331 and a backbone plasmid that is identical to the plasmid pPIGDM1 from Enterobacter agglomerans. The multidrug-resistance transposon Tn1331, which confers resistance to aminoglycoside and beta lactam antibiotics, has been previously isolated only from Klebsiella. The Enterobacter plasmid pPIGDM1, which carries a ColE1-like origin of replication and has no apparent selective marker, appears to provide a backbone for propagation of Tn1331 in Enterobacter. The recognition sequence of Tn1331 transposase for insertion into pPIGDM1 is the pentanucleotide TATTA, which occurs only once throughout the length of this plasmid.Conclusion: Transposition of Tn1331 into

Role of Myofibril-Inducing RNA in cardiac TnT expression in developing Mexican axolotl

Science.gov (United States)

Sferrazza, Gian-Franco; Zhang, Chi; Jia, Pingping; Lemanski, Sharon L.; Athauda, Gagani; Stassi, Alyssa; Halager, Kristine; Maier, Jennifer A.; Rueda-de-Leon, Elena; Gupta, Amit; Dube, Syamalima; Huang, Xupei; Prentice, Howard M.; Dube, Dipak K.; Lemanski, Larry F.

2007-01-01

The Mexican axolotl, Ambystoma mexicanum, has been a useful animal model to study heart development and cardiac myofibrillogenesis. A naturally-occurring recessive mutant, gene “c”, for cardiac non-function in the Mexican axolotl causes a failure of myofibrillogenesis due to a lack of tropomyosin expression in homozygous mutant (c/c) embryonic hearts.. Myofibril-Inducing RNA (MIR) rescues mutant hearts in vitro by promoting tropomyosin expression and myofibril formation thereafter. We have studied the effect of MIR on the expression of various isoforms of cardiac Troponin-T (cTnT), a component of the thin filament that binds with tropomyosin. Four alternatively spliced cTnT isoforms have been characterized from developing axolotl heart. The expression of various cTnT isoforms in normal, mutant, and mutant hearts corrected with MIR, is evaluated by real-time RT-PCR using isoform specific primer pairs; MIR affects the total transcription as well as the splicing of the cTnT in axolotl heart PMID:17408593

Japanese version transport/storage packaging 'TN24'

International Nuclear Information System (INIS)

Kakunai, H.; Iida, T.; Tsuda, K.; Akamatsu, H.

1993-01-01

Since 1983, Kobe Steel has been engaged jointly with the French company Transnucleaire in the development of 'TN24', a dry-type transport and storage packaging for irradiated fuel elements. This report describes the packaging, which has been adapted for use in domestic power stations using BWRs on the basis of the results of this development. (J.P.N.)

Heavy Metal Blood Test: MedlinePlus Lab Test Information

Science.gov (United States)

... this page: https://medlineplus.gov/labtests/heavymetalbloodtest.html Heavy Metal Blood Test To use the sharing features ... this page, please enable JavaScript. What is a Heavy Metal Blood Test? A heavy metal blood test ...

Peri-operative troponin monitoring using a prototype high-sensitivity cardiac troponin I (hs-cTnI) assay: comparisons with hs-cTnT and contemporary cTnI assays.

LENUS (Irish Health Repository)

Lee, Graham R

2013-09-18

Non-cardiac surgery is associated with major vascular complications and higher incidences of elevated plasma troponin (cTn) concentration. Goal-directed therapy (GDT) is a stroke volume (SV)-guided approach to intravenous (IV) fluid therapy that improves tissue perfusion, oxygenation and reduces post-operative complications. In patients undergoing major gastro-intestinal surgery, we compared high sensitive and contemporary troponin assays and correlated results with patient outcome.

A NEW CELL CLONE DERIVED FROM TRICHOPLUSIA NI TN5B1-4 CELLS

Institute of Scientific and Technical Information of China (English)

Jian-xiaoTian; Chang-youLi; Gui-lingZheng; Guo-xunLi; PingWang; Granados

2004-01-01

The characteristics of a cultured cell line do not always remain stable and may change upon continuous passage. Most continuous cell lines, even after cloning, possess several genotypes that are constantly changing. There are numerous selective and adaptive culture processes, in addition to genetic instability, that may improve phenotypic change in cell growth, virus susceptibility, gene expression, and production of virus. Similar detrimental effects of long term passaging of insect cells have also been reported for continuous cell lines, for example, Tn5B 1-4 cells, which are the most widely used for the baculovirus expression vector system (BEVS), provide superior production of recombinant proteins,however, this high productivity may be more evident in low passage cells. In this paper, we describe the isolation of a cell clone, Tn5B-40, from low passage Tn5B 1-4 cells. The growth characteristics,productions of virus, and high level of recombinant protein productions were determined. The results showed the susceptibility of both clone and Tn5B 1-4 cells to wild-type AcNPV was approximately the same rate with over 95% of infection; when the cloned cells were infected with recombinant baculoviruses expressing β-galactosidase and secreted alkaline phosphatase (SEAP), expression of the recombinant proteins from the cloned cells exceeded that from the parental Tn5B 1-4 cells.

Analysis of Tn antigenicity with a panel of new IgM and IgG1 monoclonal antibodies raised against leukemic cells

DEFF Research Database (Denmark)

Blixt, Klas Ola; Lavrova, Olga I; Mazurov, Dmitriy V

2012-01-01

antigenicity remain poorly understood. As a consequence, a broad variety of anti-Tn monoclonal antibodies (mAbs) have been generated. To further investigate the nature and complexity of the Tn antigen, we generated seven different anti-Tn mAbs of IgM and IgG classes raised against human Jurkat T cells, which...... are Tn-positive due to the low activity of T-synthase and mutation in specific chaperone Cosmc. The binding analysis of anti-Tn mAbs with the array of synthetic saccharides, glycopeptides and O-glycoproteins revealed unexpected differences in specificities of anti-Tn mAbs. IgM mAbs bound the terminal Gal...

Transposition of Tn5096 from a temperature-sensitive transducible plasmid in Streptomyces spp.

OpenAIRE

McHenney, M A; Baltz, R H

1991-01-01

Transposon Tn5096 was inserted into a derivative of the temperature-sensitive plasmid pMT660 containing the bacteriophage FP43 pac site. The resulting plasmid, pRHB126, was transduced by FP43 into several Streptomyces species. Tn5096 transposed from pRHB126 into different sites in the genomes of Streptomyces ambofaciens, Streptomyces cinnamonensis, Streptomyces coelicolor A3(2), Streptomyces fradiae, Streptomyces griseofuscus, and Streptomyces thermotolerans.

A inter-relação das sialomucinas (antígenos Tn e Stn com o adenocarcinoma no esôfago de Barrett Relationship of the sialomucins (Tn and Stn antigens with adenocarcinoma in Barrett's esophagus

Directory of Open Access Journals (Sweden)

Plínio Conte de Faria Júnior

2007-08-01

Full Text Available OBJETIVO: O esôfago de Barrett (EB é conseqüência do refluxo gastroesofágico crônico e considerado fator de risco para o desenvolvimento de adenocarcinoma. Estudos do muco, em especial das mucinas ácidas representadas pelas sialomucinas presentes nas células caliciformes, mostraram que na metaplasia do tipo intestinal, o epitélio do órgão pode expressar antígenos denominados Tn e Stn. Estes antígenos já foram analisados em tumores gástricos e colônicos, porém não foram encontradas referências à sua utilização no EB. Este trabalho objetivou analisar estes antígenos em doentes com EB e em adenocarcinoma associado ao EB. MÉTODOS: Foram estudados, utilizando testes imunohistoquímicos, os antígenos Tn e Stn, nas biópsias endoscópicas de 29 doentes com EB, sete com adenocarcinoma no EB, além de oito indivíduos com epitélio esofágico normal. RESULTADOS: Nas células caliciformes, foi observada positividade para Stn em 100% dos casos e para Tn em 48% dos casos. Nas células colunares, o Stn foi sempre negativo, enquanto o Tn foi positivo em 100% dos casos. Entretanto, nos doentes com adenocarcinoma no EB, a positividade para ambos os antígenos foi de 100%. Nos indivíduos normais, houve positividade para o antígeno Tn e negatividade para Stn em todos os casos (100%. CONCLUSÃO: É provável que nos doentes com EB a positividade para o Tn, à semelhança do ocorrido quanto à positividade do mesmo antígeno nos portadores de adenocarcinoma, possa significar maior suscetibilidade para ocorrência futura de câncer. Assim, a pesquisa das sialomucinas poderá ser rotineiramente utilizada, contribuindo como fator prognóstico para desenvolvimento de adenocarcinoma no EB.OBJECIVE: Barrett's esophagus (BE is a consequence of chronic gastroesophageal reflux and is considered a risk factor for adenocarcinoma. The study of the mucus, especially acid mucins, such as the sialomucins in the goblet cells which characterize BE, showed

Fingerprints of field-induced Berezinskii–Kosterlitz–Thouless transition in quasi-two-dimensional S=1/2 Heisenberg magnets Cu(en)(H2O)2SO4 and Cu(tn)Cl2

International Nuclear Information System (INIS)

Baranová, Lucia; Orendáčová, Alžbeta; Čižmár, Erik; Tarasenko, Róbert; Tkáč, Vladimír; Orendáč, Martin; Feher, Alexander

2016-01-01

Organo-metallic compounds Cu(en)(H 2 O) 2 SO 4 (en=C 2 H 8 N 2 ) and Cu(tn)Cl 2 (tn=C 3 H 10 N 2 ) representing S=1/2 quasi-two-dimensional Heisenberg antiferromagnets with an effective intra-layer exchange coupling J/k B ≈3 K, have been examined by specific heat measurements at temperatures down to nominally 50 mK and magnetic fields up to 14 T. A comparative analysis of magnetic specific heat in zero magnetic field revealed nearly identical contribution of short-range magnetic correlations and significant differences were observed at lowest temperatures. A phase transition to long-range order was observed in Cu(en)(H 2 O) 2 SO 4 at T C =0.9 K while hidden in Cu(tn)Cl 2 . A response of both compounds to the application of magnetic field has rather universal features characteristic for a field-induced Berezinskii–Kosterlitz–Thouless transition theoretically predicted for ideal two-dimensional magnets. - Highlights: • Magnetic specific heat of Cu(en)(H 2 O) 2 SO 4 (1) and Cu(tn)Cl 2 (2) was analysed. • In zero magnetic field, (1) and (2) behave as quasi-two-dimensional magnets. • We observed universal thermodynamic response of (1) and (2) to applied field. • Features of field-induced Berezinskii–Kosterlitz–Thouless transition were detected.

Advanced HVAC modeling with FemLab/Simulink/MatLab

NARCIS (Netherlands)

Schijndel, van A.W.M.

2003-01-01

The combined MatLab toolboxes FemLab and Simulink are evaluated as solvers for HVAC problems based on partial differential equations (PDEs). The FemLab software is designed to simulate systems of coupled PDEs, 1-D, 2-D or 3-D, nonlinear and time dependent. In order to show how the program works, a

The Breast Cancer-Associated Glycoforms of MUC1, MUC1-Tn and sialyl-Tn, Are Expressed in COSMC Wild-Type Cells and Bind the C-Type Lectin MGL.

Directory of Open Access Journals (Sweden)

Richard Beatson

Full Text Available Aberrant glycosylation occurs in the majority of human cancers and changes in mucin-type O-glycosylation are key events that play a role in the induction of invasion and metastases. These changes generate novel cancer-specific glyco-antigens that can interact with cells of the immune system through carbohydrate binding lectins. Two glyco-epitopes that are found expressed by many carcinomas are Tn (GalNAc-Ser/Thr and STn (NeuAcα2,6GalNAc-Ser/Thr. These glycans can be carried on many mucin-type glycoproteins including MUC1. We show that the majority of breast cancers carry Tn within the same cell and in close proximity to extended glycan T (Galβ1,3GalNAc the addition of Gal to the GalNAc being catalysed by the T synthase. The presence of active T synthase suggests that loss of the private chaperone for T synthase, COSMC, does not explain the expression of Tn and STn in breast cancer cells. We show that MUC1 carrying both Tn or STn can bind to the C-type lectin MGL and using atomic force microscopy show that they bind to MGL with a similar dead adhesion force. Tumour associated STn is associated with poor prognosis and resistance to chemotherapy in breast carcinomas, inhibition of DC maturation, DC apoptosis and inhibition of NK activity. As engagement of MGL in the absence of TLR triggering may lead to anergy, the binding of MUC1-STn to MGL may be in part responsible for some of the characteristics of STn expressing tumours.

76 FR 35909 - Temporary Concession Contract for Big South Fork National Recreation Area, TN/KY

Science.gov (United States)

2011-06-20

... Recreation Area, TN/KY. SUMMARY: Pursuant to 36 CFR 51.24, public notice is hereby given that the National... Concession Contract for Big South Fork National Recreation Area, TN/KY AGENCY: National Park Service... services within Big South Fork National Recreation Area, Tennessee and Kentucky, for a term not to exceed 3...

A critical evaluation of the Beckman Coulter Access hsTnI: Analytical performance, reference interval and concordance.

Science.gov (United States)

Pretorius, Carel J; Tate, Jillian R; Wilgen, Urs; Cullen, Louise; Ungerer, Jacobus P J

2018-05-01

We investigated the analytical performance, outlier rate, carryover and reference interval of the Beckman Coulter Access hsTnI in detail and compared it with historical and other commercial assays. We compared the imprecision, detection capability, analytical sensitivity, outlier rate and carryover against two previous Access AccuTnI assay versions. We established the reference interval with stored samples from a previous study and compared the concordances and variances with the Access AccuTnI+3 as well as with two commercial assays. The Access hsTnI had excellent analytical sensitivity with the calibration slope 5.6 times steeper than the Access AccuTnI+3. The detection capability was markedly improved with the SD of the blank 0.18-0.20 ng/L, LoB 0.29-0.33 ng/L and LoD 0.58-0.69 ng/L. All the reference interval samples had a result above the LoB value. At a mean concentration of 2.83 ng/L the SD was 0.28 ng/L (CV 9.8%). Carryover (0.005%) and outlier (0.046%) rates were similar to the Access AccuTnI+3. The combined male and female 99th percentile reference interval was 18.2 ng/L (90% CI 13.2-21.1 ng/L). Concordance amongst the assays was poor with only 16.7%, 19.6% and 15.2% of samples identified by all 4 assays as above the 99th, 97.5th and 95th percentiles. Analytical imprecision was a minor contributor to the observed variances between assays. The Beckman Coulter Access hsTnI assay has excellent analytical sensitivity and precision characteristics close to zero. This allows cTnI measurement in all healthy individuals and the capability to identify numerically small differences between serial samples as statistically significant. Concordance in healthy individuals remains poor amongst assays. Crown Copyright © 2018. Published by Elsevier Inc. All rights reserved.

Experimental manipulation of TN:TP ratiossuppress cyanobacterial biovolume and microcystinconcentration in large-scale in situ mesocosms

Science.gov (United States)

Harris, Theodore D.; Wilhelm, Frank M.; Graham, Jennifer L.; Loftin, Keith A.

2014-01-01

A global dataset was compiled to examine relations between the total nitrogen to total phosphorus ratio (TN:TP) and microcystin concentration in lakes and reservoirs. Microcystin concentration decreased as TN:TP ratios increased, suggesting that manipulation of the TN:TP ratio may reduce microcystin concentrations. This relationship was experimentally tested by adding ammonium nitrate to increase the TN:TP ratio in large-scale (70 m3), in situ mesocosms located in a eutrophic reservoir that routinely experiences toxic blooms of cyanobacteria. At a TN:TP ratio >75:1, chlorophytes dominated the phytoplankton community in the mesocosms, while cyanobacterial biovolume was significantly reduced and microcystin was not detected. In contrast, the unmanipulated reservoir was dominated by cyanobacteria, and microcystin was detected. Secchi depths were 1.1 to 1.8 times greater in the mesocosms relative to the reservoir. Cladoceran zooplankton had a larger body size (0.14 mm on average) in the mesocosms compared to conspecifics in the reservoir, which was likely related to the higher quality food. Combined, these empirical and experimental data indicate that although nutrient addition is counterintuitive to current cyanobacteria management practices, increasing the TN:TP ratio by adding nitrogen may be a potential short-term management strategy to reduce cyanobacteria and cyanotoxins when other alternatives (e.g., phosphorus reduction) are not possible. Additional experimental studies with careful controls are needed to define best management practices and identify any potential unintended consequences before nitrogen addition is implemented as a lake and reservoir management practice.

Vision Lab

Data.gov (United States)

Federal Laboratory Consortium — The Vision Lab personnel perform research, development, testing and evaluation of eye protection and vision performance. The lab maintains and continues to develop...

The TN-GEMINI: experience on a versatile alpha waste transport container

International Nuclear Information System (INIS)

Roland, V.; Chanzy, Y.

2001-01-01

The present paper discusses experience gained in moving alpha wastes and its teachings regarding transport aspects of D and D. Alpha wastes are generated in fuel cycle facilities such as those involved in reprocessing, in manufacture of mixed oxide fuel, and by research laboratories. If a significant amount of wastes has to be transported, then a Type B packaging is required. Developed by Transnucleaire and COGEMA, the TN GEMINI container enables nuclear facilities operators to optimise their alpha waste transport management, and more generally contribute to their D and D projects. After describing succinctly the design of the TN GEMINI, the paper will explain how the packaging is being operated. Teachings from experience will be shared. (orig.)

Designing inquiry learning spaces for online labs in the Go-Lab platform

NARCIS (Netherlands)

de Jong, Ton; Gillet, Dennis; Sotiriou, Sofoklis; Agogi, Ellinogermaniki; Zacharia, Zacharias

2015-01-01

The Go-Lab project (http://www.go-lab-project.eu/) aims to enable the integration of online labs through inquiry-based learning approaches into science classrooms. Through the use of an advanced plug and play technological solution the Go-Lab project opens up remote science laboratories, data

Development Of Protocols For Simultaneous Measurements Of Rn, Tn Using Nuclear Track Detectors And Trial Application In Mining Areas Of Vietnam

International Nuclear Information System (INIS)

Bui Dac Dung; Trinh Van Giap; Le Dinh Cuong; Tran Khanh Minh; Nguyen Huu Quyet; Nguyen Van Khanh; Tran Ngoc Toan

2013-01-01

Radioactive gases Radon (Rn) and Thoron (Tn) contribute of more than 50% of natural radiation dose. However, separate measurements of Rn and Tn have not been paid enough attention. An Intergovernmental Cooperation Project was conducted at the Institute for Nuclear Science and Technology with the help from Hungarian experts. The main tasks of the project were to finalize 02 protocols for simultaneous measurements of Rn and Tn using nuclear track detectors and to test these protocols in investigating the concentrations of Rn and Tn, calculating the natural dose due to Rn and Tn, and evaluating the increased radiation dose and radiation safety due to mining activities. Main results of the project include 02 protocols for simultaneous measurements of Rn and Tn and the test results in the mining areas. Rn and Tn concentrations inside the coal mining tunnels are in the average level of Vietnam and the world. Tn concentration inside the factory for separating Zircon at the Ha Tinh Zircon Processing Plant was found to be very high, up to 2931 Bq/m 3 . Based on annual effective dose calculation, workers inside the factory for separating Zircon at the Ha Tinh Zircon Processing Plant could receive an annual effective dose due to Rn and Tn of 4.890 mSv/year, and the increasing dose of 4.710 mSv/year is for higher than 1 mSv/year recommended by the IAEA. (author)

The Breast Cancer-Associated Glycoforms of MUC1, MUC1-Tn and sialyl-Tn, Are Expressed in COSMC Wild-Type Cells and Bind the C-Type Lectin MGL

DEFF Research Database (Denmark)

Beatson, Richard; Maurstad, Gjertrud; Picco, Gianfranco

2015-01-01

Aberrant glycosylation occurs in the majority of human cancers and changes in mucin-type O-glycosylation are key events that play a role in the induction of invasion and metastases. These changes generate novel cancer-specific glyco-antigens that can interact with cells of the immune system through...... carbohydrate binding lectins. Two glyco-epitopes that are found expressed by many carcinomas are Tn (GalNAc-Ser/Thr) and STn (NeuAcα2,6GalNAc-Ser/Thr). These glycans can be carried on many mucin-type glycoproteins including MUC1. We show that the majority of breast cancers carry Tn within the same cell...

Physical-chemical treatment of rainwater runoff in recovery and recycling companies: lab-scale investigation.

Science.gov (United States)

Blondeel, E; De Wandel, S; Florin, R; Hugelier, S; Chys, M; Depuydt, V; Folens, K; Du Laing, G; Verliefde, A; Van Hulle, S W H

2017-08-10

Scrap material recovery and recycling companies are producing wastewater in which common pollutants (such as COD, nutrients and suspended solids), toxic metals, polyaromatic hydrocarbons (PAH) and polychlorinated biphenyls (PCB) frequently can exceed the discharge limits. Lab-scale optimisation of different possible physical-chemical treatment techniques was performed on the wastewater originating from three different companies in view of further testing at pilot-scale testing and implementation at full-scale. The lab-scale tests demonstrate that sedimentation or hydrocyclone treatment as stand-alone technique cannot be used for proper treatment of this type of wastewater. Dual bed filtration or coagulation/flocculation proved to be more promising with removal efficiencies of about 71-95% (dual bed filtration) and 61-97% (coagulation/flocculation) for the above-mentioned pollutants (metals, PAH and PCB).

Induction of the Tn10 Precise Excision in E. coli Cells after Accelerated Heavy Ions Irradiation

CERN Document Server

Zhuravel, D V

2003-01-01

The influence of the irradiation of different kinds on the indication of the structural mutations in the bacteria Escherichia coli is considered. The regularities of the Tn10 precise excision after accelerated ^{4}He and ^{12}C ions irradiations with different linear energy transfer (LET) were investigated. Dose dependences of the survival and relative frequency of the Tn10 precise excision were obtained. It was shown, that the relative frequency of the Tn10 precise excision is the exponential function from the irradiation dose. Relative biological efficiency (RBE), and relative genetic efficiency (RGE) were calculated, and were treated as the function of the LET.

Moving the Plasmon of LaB6 from IR to Near-IR via Eu-Doping

Directory of Open Access Journals (Sweden)

Tracy M. Mattox

2018-02-01

Full Text Available Lanthanum hexaboride (LaB6 has become a material of intense interest in recent years due to its low work function, thermal stability and intriguing optical properties. LaB6 is also a semiconductor plasmonic material with the ability to support strong plasmon modes. Some of these modes uniquely stretch into the infrared, allowing the material to absorb around 1000 nm, which is of great interest to the window industry. It is well known that the plasmon of LaB6 can be tuned by controlling particle size and shape. In this work, we explore the options available to further tune the optical properties by describing how metal vacancies and Eu doping concentrations are additional knobs for tuning the absorbance from the near-IR to far-IR in La1−xEuxB6 (x = 0, 0.2, 0.5, 0.8, and 1.0. We also report that there is a direct correlation between Eu concentration and metal vacancies within the Eu1−xLaxB6.

Tn7::In2-8 dispersion in multidrug resistant isolates of Acinetobacter baumannii from Chile Dispersión de Tn7::In2-8 en aislamientos multirresistentes de Acinetobacter baumannii de Chile

Directory of Open Access Journals (Sweden)

M. S. Ramírez

2010-06-01

Full Text Available Acinetobacter baumannii is considered an important pathogen in our hospital environment having a well-known capacity to acquire different mechanisms of antibiotic resistance. Previous studies in our laboratory had exposed the high dispersion of class 2 integrons in this species. In the present study, we analyzed 7 multiresistant intI2 positive A. baumannii isolates, 6 of which were found to harbour the Tn7::In2-8 element. Our results demonstrate the unusually high distribution of Tn7::In2-8 among different A. baumannii clones from Chile, suggesting a particular behavior of these elements at geographical level.Acinetobacter baumannii, patógeno de importancia clínica en el ámbito hospitalario, es reconocido como un microorganismo que posee la capacidad de evolucionar rápidamente hacia la multirresistencia. Estudios previos efectuados en nuestro laboratorio han demostrado la alta dispersión de los integrones de clase 2 en aislamientos de esta especie. En el presente trabajo se analizaron 7 aislamientos de Acinetobacter baumannii multirresistentes portadores de la integrasa de clase 2, 6 de los cuales portaban el inusual arreglo Tn7::In2-8. Nuestros resultados muestran una elevada frecuencia de dispersión del elemento Tn7::In2-8 en diferentes clones circulantes en Chile, lo que sugiere un comportamiento geográfico particular.

Innovations in STEM education: the Go-Lab federation of online labs

NARCIS (Netherlands)

de Jong, Anthonius J.M.; Sotiriou, Sofoklis; Gillet, Dennis

2014-01-01

The Go-Lab federation of online labs opens up virtual laboratories (simulation), remote laboratories (real equipment accessible at distance) and data sets from physical laboratory experiments (together called “online labs”) for large-scale use in education. In this way, Go-Lab enables inquiry-based

Impacts of climate change on TN load and its control in a River Basin with complex pollution sources.

Science.gov (United States)

Yang, Xiaoying; Warren, Rachel; He, Yi; Ye, Jinyin; Li, Qiaoling; Wang, Guoqing

2018-02-15

It is increasingly recognized that climate change could affect the quality of water through complex natural and anthropogenic mechanisms. Previous studies on climate change and water quality have mostly focused on assessing its impact on pollutant loads from agricultural runoff. A sub-daily SWAT model was developed to simulate the discharge, transport, and transformation of nitrogen from all known anthropogenic sources including industries, municipal sewage treatment plants, concentrated and scattered feedlot operations, rural households, and crop production in the Upper Huai River Basin. This is a highly polluted basin with total nitrogen (TN) concentrations frequently exceeding Class V of the Chinese Surface Water Quality Standard (GB3838-2002). Climate change projections produced by 16 Global Circulation Models (GCMs) under the RCP 4.5 and RCP 8.5 scenarios in the mid (2040-2060) and late (2070-2090) century were used to drive the SWAT model to evaluate the impacts of climate change on both the TN loads and the effectiveness of three water pollution control measures (reducing fertilizer use, constructing vegetative filter strips, and improving septic tank performance) in the basin. SWAT simulation results have indicated that climate change is likely to cause an increase in both monthly average and extreme TN loads in February, May, and November. The projected impact of climate change on TN loads in August is more varied between GCMs. In addition, climate change is projected to have a negative impact on the effectiveness of septic tanks in reducing TN loads, while its impacts on the other two measures are more uncertain. Despite the uncertainty, reducing fertilizer use remains the most effective measure for reducing TN loads under different climate change scenarios. Meanwhile, improving septic tank performance is relatively more effective in reducing annual TN loads, while constructing vegetative filter strips is more effective in reducing annual maximum monthly

Highly efficient full-length hepatitis C virus genotype 1 (strain TN) infectious culture system

DEFF Research Database (Denmark)

Li, Yi-Ping; Ramirez, Santseharay; Jensen, Sanne B

2012-01-01

Chronic infection with hepatitis C virus (HCV) is an important cause of end stage liver disease worldwide. In the United States, most HCV-related disease is associated with genotype 1 infection, which remains difficult to treat. Drug and vaccine development was hampered by inability to culture...... full-length TN infection dose-dependently. Given the unique importance of genotype 1 for pathogenesis, this infectious 1a culture system represents an important advance in HCV research. The approach used and the mutations identified might permit culture development for other HCV isolates, thus......) culture systems in Huh7.5 cells. Here, we developed a highly efficient genotype 1a (strain TN) full-length culture system. We initially found that the LSG substitutions conferred viability to an intergenotypic recombinant composed of TN 5' untranslated region (5'UTR)-NS5A and JFH1 NS5B-3'UTR; recovered...

Transitioning high sensitivity cardiac troponin I (hs-cTnI) into routine diagnostic use: More than just a sensitivity issue

LENUS (Irish Health Repository)

Lee, Graham R

2016-04-01

High sensitivity cardiac troponin T and I (hs-cTnT and hs-cTnI) assays show analytical, diagnostic and prognostic improvement over contemporary sensitive cTn assays. However, given the importance of troponin in the diagnosis of myocardial infarction, implementing this test requires rigorous analytical and clinical verification across the total testing pathway. This was the aim of this study.

PD Lab

NARCIS (Netherlands)

Bilow, Marcel; Entrop, Alexis Gerardus; Lichtenberg, Jos; Stoutjesdijk, Pieter

2015-01-01

PD Lab explores the applications of building sector related product development. PD lab investigates and tests digital production technologies like CNC milled wood connections. It will also act as a platform in its wider meaning to investigate the effects and influences of file to factory

Establishing exchange bias below T-N with polycrystalline Ni0.52Co0.48O/Co bilayers

DEFF Research Database (Denmark)

Berkowitz, A.E.; Hansen, Mikkel Fougt; Tang, Y.J.

2005-01-01

Exchange-coupled bilayers of polycrystalline ferromagnetic (FM) Co on antiferromagnetic (AFM) Ni0.52Co0.48O were investigated with emphasis on the issue of establishing an exchange-bias field, H-E, below the AFM ordering temperature, T-N. It was found that field-cooling the bilayers through T-N p...

78 FR 54168 - Special Local Regulation, Cumberland River, Mile 157.0 to 159.0; Ashland City, TN

Science.gov (United States)

2013-09-03

... Local Regulation, Cumberland River, Mile 157.0 to 159.0; Ashland City, TN AGENCY: Coast Guard, DHS... Special local regulation; Cumberland River, Miles 157.0 to 159.0, Ashland City, TN. (a) Location. The... regulation for the waters of the Cumberland River beginning at mile marker 157.0 and ending at mile marker...

Kinematic Labs with Mobile Devices

Science.gov (United States)

Kinser, Jason M.

2015-07-01

This book provides 13 labs spanning the common topics in the first semester of university-level physics. Each lab is designed to use only the student's smartphone, laptop and items easily found in big-box stores or a hobby shop. Each lab contains theory, set-up instructions and basic analysis techniques. All of these labs can be performed outside of the traditional university lab setting and initial costs averaging less than 8 per student, per lab.

Optimization of high-rate TN removal in a novel constructed wetland integrated with microelectrolysis system treating high-strength digestate supernatant.

Science.gov (United States)

Guo, Luchen; He, Keli; Wu, Shubiao; Sun, Hao; Wang, Yanfei; Huang, Xu; Dong, Renjie

2016-08-01

The potential of high-rate TN removal in three aerated horizontal subsurface-flow constructed wetlands to treat high-strength anaerobic digestate supernatant was evaluated. Different strategies of intermittent aeration and effluent recirculation were applied to compare their effect on nitrogen depuration performance. Additional glucose supply and iron-activated carbon based post-treatment systems were established and examined, respectively, to further remove nitrate that accumulated in the effluents from aerated wetlands. The results showed that intermittent aeration (1 h on:1 h off) significantly improved nitrification with ammonium removal efficiency of 90% (18.1 g/(m(2)·d)), but limited TN removal efficiency (53%). Even though effluent recirculation (a ratio of 1:1) increased TN removal from 53% to 71%, the effluent nitrate concentration was still high. Additional glucose was used as a post-treatment option and further increased the TN removal to 82%; however, this implementation caused additional organic pollution. Furthermore, the iron-activated carbon system stimulated with a microelectrolysis process achieved greater than 85% effluent nitrate removal and resulted in 86% TN removal. Considering the high TN removal rate, aerated constructed wetlands integrated with a microelectrolysis-driven system show great potential for treating high-strength digestate supernatant. Copyright © 2016 Elsevier Ltd. All rights reserved.

Teachers' Perspectives on Online Virtual Labs vs. Hands-On Labs in High School Science

Science.gov (United States)

Bohr, Teresa M.

This study of online science teachers' opinions addressed the use of virtual labs in online courses. A growing number of schools use virtual labs that must meet mandated laboratory standards to ensure they provide learning experiences comparable to hands-on labs, which are an integral part of science curricula. The purpose of this qualitative case study was to examine teachers' perceptions of the quality and effectiveness of high school virtual labs. The theoretical foundation was constructivism, as labs provide student-centered activities for problem solving, inquiry, and exploration of phenomena. The research questions focused on experienced teachers' perceptions of the quality of virtual vs. hands-on labs. Data were collected through survey questions derived from the lab objectives of The Next Generation Science Standards . Eighteen teachers rated the degree of importance of each objective and also rated how they felt virtual labs met these objectives; these ratings were reported using descriptive statistics. Responses to open-ended questions were few and served to illustrate the numerical results. Many teachers stated that virtual labs are valuable supplements but could not completely replace hands-on experiences. Studies on the quality and effectiveness of high school virtual labs are limited despite widespread use. Comprehensive studies will ensure that online students have equal access to quality labs. School districts need to define lab requirements, and colleges need to specify the lab experience they require. This study has potential to inspire positive social change by assisting science educators, including those in the local school district, in evaluating and selecting courseware designed to promote higher order thinking skills, real-world problem solving, and development of strong inquiry skills, thereby improving science instruction for all high school students.

OpenLabNotes

DEFF Research Database (Denmark)

List, Markus; Franz, Michael; Tan, Qihua

2015-01-01

be advantageous if an ELN was Integrated with a laboratory information management system to allow for a comprehensive documentation of experimental work including the location of samples that were used in a particular experiment. Here, we present OpenLabNotes, which adds state-of-the-art ELN capabilities to Open......LabFramework, a powerful and flexible laboratory information management system. In contrast to comparable solutions, it allows to protect the intellectual property of its users by offering data protection with digital signatures. OpenLabNotes effectively Closes the gap between research documentation and sample management......, thus making Open-Lab Framework more attractive for laboratories that seek to increase productivity through electronic data management....

Superior Predictive Value for NTproBNP Compared with High Sensitivity cTnT in Dialysis Patients: A Pilot Prospective Observational Study

Directory of Open Access Journals (Sweden)

Luminita Voroneanu

2014-12-01

Full Text Available Background/Aims: The clinical utility of the new biomarker, high sensitivity cardiac T troponin (hs-cTnT is still unclear in dialysis patients. Furthermore, the prognostic value of combining N-terminal pro-B-type natriuretic peptide (NT-pro-BNP and hs-cTnT has not been explored so far. The objective of this pilot study was to determine the utility of hs-cTnT alone versus hs-cTnT in combination with NT-proBNP for predicting death in a stable hemodialysis cohort. Methods: A prospective observational pilot study including 98 chronic asymptomatic hemodialysis patients with a follow up period of 24 months was designed. The cut-off values for NT-proBNP and hs-cTnT were calculated using receiver operating characteristic (ROC analysis, using mortality as an end-point. Based on the cut-off values, the cohort was divided into four groups. Group 1 - NT-proBNP 69.48 ng/l; group 3 - NT-proBNP > 14275 pg/ml and hs-cTnT 14275 pg/ml and hs-cTnT > 69.48 ng/l. Survival for each group was determined using the Kaplan-Meier method and Cox regression analysis. Results: During the follow-up period 16 patients died. According to the ROC curves analysis, the cut-off point for hs-cTnT and for NT-proBNP were 69.43 ng/l (AUC = 0.618; p = 0.04 and 14275 pg/ml (AUC = 0.722; p = 0.003, respectively. In univariate Cox analysis, both hs-cTnT (HR = 3.34; p = 0.016 and NT-proBNP (HR = 5.94; p = 0.01 were predictors of death. In the multivariable Cox proportional hazards model, only NT-pro-BNP levels above the cut-off value remained an independent predictor of all-cause mortality. The combined elevation of both biomarkers did not improve significantly the prognostic value compared with NT-proBNP alone (HR = 6.15 versus HR =4 .78; p = 0.338. Conclusion: NT-pro-BNP is a strong predictor of overall mortality in asymptomatic hemodialysis patients. The addition of hs-cTnT did not improve the prognostic accuracy compared with NT proBNP alone.

Clinical application of combined detection of serum hs-CRP, GMP-140 and cTnI in patients with coronary heart diseases

International Nuclear Information System (INIS)

Qin Jibao; Wu Zhaozeng

2010-01-01

Objective: To explore the clinical significance of changes of serum hs-CRP, GMP-140 and cTnI levels in patients with coronary heart diseases. Methods: Serum GMP-140 (with RIA), cTnI (with ELISA) and hs-CRP (with immuno turbidity method) levels were determined in 91 patients with coronary heart diseases (42 SAP, 34UAP, 15AMI) and 35 controls. Results: Serum hs-CRP, GMP-140, cTnI levels in patients with coronary heart diseases were significantly higher than those in controls (P <0.01). Among the patients with of coronary heart diseases, the magnitude of changes of the levels of serum hs-CRP, GMP-140 and cTnI levels in AMI and UAP groups were significantly larger than those in SAP group (P < 0.05). Serum hs-CRP levels were positively correlated with serum GMP-140 and cTnI levels (r = 0.6214, 0.6023, P < 0.01). Conclusion: Serum hs-CRP, GMP140 and cTnI levels were closely related to the diseases process of coronary heart diseases and were of great clinical importance for assessment of the disease and outcome prediction. (authors)

A comparative study on real lab and simulation lab in communication engineering from students' perspectives

Science.gov (United States)

Balakrishnan, B.; Woods, P. C.

2013-05-01

Over the years, rapid development in computer technology has engendered simulation-based laboratory (lab) in addition to the traditional hands-on (physical) lab. Many higher education institutions adopt simulation lab, replacing some existing physical lab experiments. The creation of new systems for conducting engineering lab activities has raised concerns among educators on the merits and shortcomings of both physical and simulation labs; at the same time, many arguments have been raised on the differences of both labs. Investigating the effectiveness of both labs is complicated, as there are multiple factors that should be considered. In view of this challenge, a study on students' perspectives on their experience related to key aspects on engineering laboratory exercise was conducted. In this study, the Visual Auditory Read and Kinetic model was utilised to measure the students' cognitive styles. The investigation was done through a survey among participants from Multimedia University, Malaysia. The findings revealed that there are significant differences for most of the aspects in physical and simulation labs.

Virtual Reality Lab Assistant

Science.gov (United States)

Saha, Hrishikesh; Palmer, Timothy A.

1996-01-01

Virtual Reality Lab Assistant (VRLA) demonstration model is aligned for engineering and material science experiments to be performed by undergraduate and graduate students in the course as a pre-lab simulation experience. This will help students to get a preview of how to use the lab equipment and run experiments without using the lab hardware/software equipment. The quality of the time available for laboratory experiments can be significantly improved through the use of virtual reality technology.

Some transition metal ions complexes of tricine (Tn and amino acids: pH-titration, synthesis and antimicrobial activity

Directory of Open Access Journals (Sweden)

M.E. Zayed

2014-12-01

Full Text Available Equilibrium studies have been carried out on complex formation of M(II (M = Co(II, Cu(II and Zn(II with tricine (Tn and L = amino acids in aqueous solution, at 25 °C and ionic strength of I = 0.1 M (NaNO3. The ternary complexes of amino acids are formed by simultaneous reactions. The concentration distribution of the complexes is evaluated. The solid complexes of [M(II–Tn–Histidine (Hist] have been synthesized and characterized by elemental analysis, infrared, magnetic and conductance measurements. The synthesized complexes have been screened for their antibacterial activities and the complexes show a significant antibacterial activity against four bacterial species: Staphylococcus aureus (Gram +ve, Streptococcus pyogenesr (Gram +ve, Serratia marcescens (Gram −ve and Escherichia coli (Gram −ve. The activity increases by increasing the concentration of the complexes.

Fingerprints of field-induced Berezinskii–Kosterlitz–Thouless transition in quasi-two-dimensional S=1/2 Heisenberg magnets Cu(en)(H{sub 2}O){sub 2}SO{sub 4} and Cu(tn)Cl{sub 2}

Energy Technology Data Exchange (ETDEWEB)

Baranová, Lucia [Civil Engineering Faculty, Department of Applied Mathematics, Technical University of Košice, Vysokoškolská 4 SK-042 00, Košice (Slovakia); Orendáčová, Alžbeta, E-mail: alzbeta.orendacova@upjs.sk [Center of Low Temperature Physics, Faculty of Science, P. J. Šafárik University, Park Angelinum 9 SK-041 54, Košice (Slovakia); Čižmár, Erik [Center of Low Temperature Physics, Faculty of Science, P. J. Šafárik University, Park Angelinum 9 SK-041 54, Košice (Slovakia); Tarasenko, Róbert; Tkáč, Vladimír [Center of Low Temperature Physics, Faculty of Science, P. J. Šafárik University, Park Angelinum 9 SK-041 54, Košice (Slovakia); Charles University, Faculty of Mathematics and Physics, Ke Karlovu 5 12116, Prague (Czech Republic); Orendáč, Martin; Feher, Alexander [Center of Low Temperature Physics, Faculty of Science, P. J. Šafárik University, Park Angelinum 9 SK-041 54, Košice (Slovakia)

2016-04-15

Organo-metallic compounds Cu(en)(H{sub 2}O){sub 2}SO{sub 4} (en=C{sub 2}H{sub 8}N{sub 2}) and Cu(tn)Cl{sub 2} (tn=C{sub 3}H{sub 10}N{sub 2}) representing S=1/2 quasi-two-dimensional Heisenberg antiferromagnets with an effective intra-layer exchange coupling J/k{sub B}≈3 K, have been examined by specific heat measurements at temperatures down to nominally 50 mK and magnetic fields up to 14 T. A comparative analysis of magnetic specific heat in zero magnetic field revealed nearly identical contribution of short-range magnetic correlations and significant differences were observed at lowest temperatures. A phase transition to long-range order was observed in Cu(en)(H{sub 2}O){sub 2}SO{sub 4} at T{sub C}=0.9 K while hidden in Cu(tn)Cl{sub 2}. A response of both compounds to the application of magnetic field has rather universal features characteristic for a field-induced Berezinskii–Kosterlitz–Thouless transition theoretically predicted for ideal two-dimensional magnets. - Highlights: • Magnetic specific heat of Cu(en)(H{sub 2}O){sub 2}SO{sub 4} (1) and Cu(tn)Cl{sub 2} (2) was analysed. • In zero magnetic field, (1) and (2) behave as quasi-two-dimensional magnets. • We observed universal thermodynamic response of (1) and (2) to applied field. • Features of field-induced Berezinskii–Kosterlitz–Thouless transition were detected.

Interaction of a novel Tn (GalNAc alpha 1-->Ser/Thr) glycoprotein with Gal, GalNAc and GlcNAc specific lectins.

Science.gov (United States)

Wu, A M; Wu, J H; Shen, F

1994-01-14

A naturally occurring Tn glycoprotein (Native ASG-Tn) with GalNAc alpha 1-->Ser/Thr as the only carbohydrate side chains, has been prepared from armadillo submandibular glands. In a quantitative precipitin assay, this glycoprotein completely precipitated Maclura pomifera (MPA), Vicia villosa B4 (VVL-B4) and Artocarpus integrifolia (Jacalin, AIL). It also reacted well with Helix pomatia (HPL) and Wistaria floribunda (WFL) and precipitated over 75% of the lectin nitrogen added, but poorly with Ricinus communis agglutinin (RCA1), ricin, peanut (Arachis hypogaea, PNA), Abrus precatorius agglutinin (APA) and Triticum vulgaris (WGA). This finding suggests that this novel Tn-glycoprotein may serve as a useful reagent for differentiating Tn and T specific monoclonal antibodies and lectins.

Dynamic Equilibrium of Cardiac Troponin C's Hydrophobic Cleft and Its Modulation by Ca2+ Sensitizers and a Ca2+ Sensitivity Blunting Phosphomimic, cTnT(T204E).

Science.gov (United States)

Schlecht, William; Dong, Wen-Ji

2017-10-18

Several studies have suggested that conformational dynamics are important in the regulation of thin filament activation in cardiac troponin C (cTnC); however, little direct evidence has been offered to support these claims. In this study, a dye homodimerization approach is developed and implemented that allows the determination of the dynamic equilibrium between open and closed conformations in cTnC's hydrophobic cleft. Modulation of this equilibrium by Ca 2+ , cardiac troponin I (cTnI), cardiac troponin T (cTnT), Ca 2+ -sensitizers, and a Ca 2+ -desensitizing phosphomimic of cTnT (cTnT(T204E) is characterized. Isolated cTnC contained a small open conformation population in the absence of Ca 2+ that increased significantly upon the addition of saturating levels of Ca 2+ . This suggests that the Ca 2+ -induced activation of thin filament arises from an increase in the probability of hydrophobic cleft opening. The inclusion of cTnI increased the population of open cTnC, and the inclusion of cTnT had the opposite effect. Samples containing Ca 2+ -desensitizing cTnT(T204E) showed a slight but insignificant decrease in open conformation probability compared to samples with cardiac troponin T, wild type [cTnT(wt)], while Ca 2+ sensitizer treated samples generally increased open conformation probability. These findings show that an equilibrium between the open and closed conformations of cTnC's hydrophobic cleft play a significant role in tuning the Ca 2+ sensitivity of the heart.

EnviroAtlas - Memphis, TN - Meter-Scale Urban Land Cover (MULC) Data (2012)

Data.gov (United States)

U.S. Environmental Protection Agency — The Memphis, TN EnviroAtlas One Meter-scale Urban Land Cover (MULC) dataset comprises 2,733 km2 around the city of Memphis, surrounding towns, and rural areas. These...

LabVIEW 8 student edition

CERN Document Server

Bishop, Robert H

2007-01-01

For courses in Measurement and Instrumentation, Electrical Engineering lab, and Physics and Chemistry lab. This revised printing has been updated to include new LabVIEW 8.2 Student Edition. National Instruments' LabVIEW is the defacto industry standard for test, measurement, and automation software solutions. With the Student Edition of LabVIEW, students can design graphical programming solutions to their classroom problems and laboratory experiments with software that delivers the graphical programming capabilites of the LabVIEW professional version. . The Student Edition is also compatible with all National Instruments data acquisition and instrument control hardware. Note: The LabVIEW Student Edition is available to students, faculty, and staff for personal educational use only. It is not intended for research, institutional, or commercial use. For more information about these licensing options, please visit the National Instruments website at (http:www.ni.com/academic/)

Rapid and efficient introduction of a foreign gene into bacterial artificial chromosome-cloned varicella vaccine by Tn7-mediated site-specific transposition

International Nuclear Information System (INIS)

Somboonthum, Pranee; Koshizuka, Tetsuo; Okamoto, Shigefumi; Matsuura, Masaaki; Gomi, Yasuyuki; Takahashi, Michiaki; Yamanishi, Koichi; Mori, Yasuko

2010-01-01

Using a rapid and reliable system based on Tn7-mediated site-specific transposition, we have successfully constructed a recombinant Oka varicella vaccine (vOka) expressing the mumps virus (MuV) fusion protein (F). The backbone of the vector was our previously reported vOka-BAC (bacterial artificial chromosome) genome. We inserted the transposon Tn7 attachment sequence, LacZα-mini-attTn7, into the region between ORF12 and ORF13 to generate a vOka-BAC-Tn genome. The MuV-F expressing cassette was transposed into the vOka-BAC genome at the mini-attTn7 transposition site. MuV-F protein was expressed in recombinant virus, rvOka-F infected cells. In addition, the MuV-F protein was cleaved in the rvOka-F infected cells as in MuV-infected cells. The growth of rvOka-F was similar to that of the original recombinant vOka without the F gene. Thus, we show that Tn7-mediated transposition is an efficient method for introducing a foreign gene expression cassette into the vOka-BAC genome as a live virus vector.

R-prime site-directed transposon Tn7 mutagenesis of the photosynthetic apparatus in Rhodopseudomonas capsulata

Energy Technology Data Exchange (ETDEWEB)

Youvan, D C [Univ. of California, Berkeley; Elder, J T; Sandlin, D E; Zsebo, K; Alder, D P; Panopoulos, N J; Marrs, B L; Hearst, J E

1982-01-01

Site-directed mutagenesis of the photosynthetic apparatus (PSA) genes in Rhodopseudomonas capsulata is presented utilizing a transposon Tn7 mutagenized R-prime. The R-prime, pRPS404, bears most of the genes necessary for the differentiation of the photosynthetic apparatus. Mutagenesis of the R-prime with Tn7 in Escherichia coli, conjugation into R. capsulata, and homologous recombination with the wild-type alleles efficiently generates photosynthetic apparatus lesions. Wild-type alleles are lost spontaneously and the Tn7-induced lesions are revealed by subsequent intramolecular recombination between IS21 insertion elements that bracket the prime sequences in direct repeat. The molecular nature of the intermediates involved in the transposition, recombination and deletion have been investigated by Southern hybridization analysis. The spontaneous loss of wild-type alleles after homologous recombination with the chromosome may be of general use to other prokaryotic site-directed transposon mutagenesis schemes. The IS21-mediated deletion of the prime DNA is dependent on the RecA protein in E. coli, generating the parental R-factor bearing one IS21 element. A genetic-physical map exists for a portion of the prime photosynthetic apparatus DNA. When Tn7 is inserted into a bacteriochlorophyll gene in the R-prime and then crossed into R. capsulata, mutants are produced that accumulate a bacteriochlorophyll precursor, which is in excellent agreement with the existing genetic-physical map. This corroborates the mutagenesis scheme.

Lab at Home: Hardware Kits for a Digital Design Lab

Science.gov (United States)

Oliver, J. P.; Haim, F.

2009-01-01

An innovative laboratory methodology for an introductory digital design course is presented. Instead of having traditional lab experiences, where students have to come to school classrooms, a "lab at home" concept is proposed. Students perform real experiments in their own homes, using hardware kits specially developed for this purpose. They…

Labs not in a lab: A case study of instructor and student perceptions of an online biology lab class

Science.gov (United States)

Doiron, Jessica Boyce

Distance learning is not a new phenomenon but with the advancement in technology, the different ways of delivering an education have increased. Today, many universities and colleges offer their students the option of taking courses online instead of sitting in a classroom on campus. In general students like online classes because they allow for flexibility, the comfort of sitting at home, and the potential to save money. Even though there are advantages to taking online classes, many students and instructors still debate the effectiveness and quality of education in a distant learning environment. Many universities and colleges are receiving pressure from students to offer more and more classes online. Research argues for both the advantages and disadvantages of online classes and stresses the importance of colleges and universities weighing both sides before deciding to adopt an online class. Certain classes may not be suitable for online instruction and not all instructors are suitable to teach online classes. The literature also reveals that there is a need for more research on online biology lab classes. With the lack of information on online biology labs needed by science educators who face the increasing demand for online biology labs, this case study hopes to provide insight into the use of online biology lab classes and the how students and an instructor at a community college in Virginia perceive their online biology lab experience as well as the effectiveness of the online labs.

Antimicrobial resistance, heavy metal resistance and integron content in bacteria isolated from a South African tilapia aquaculture system.

Science.gov (United States)

Chenia, Hafizah Y; Jacobs, Anelet

2017-11-21

Antibacterial compounds and metals co-select for antimicrobial resistance when bacteria harbour resistance genes towards both types of compounds, facilitating the proliferation and evolution of antimicrobial and heavy metal resistance. Antimicrobial and heavy metal resistance indices of 42 Gram-negative bacteria from a tilapia aquaculture system were determined to identify possible correlations between these phenotypes. Agar dilution assays were carried out to determine susceptibility to cadmium, copper, lead, mercury, chromate and zinc, while susceptibility to 21 antimicrobial agents was investigated by disk diffusion assays. Presence of merA, the mercury resistance gene, was determined by dot-blot hybridizations and PCR. Association of mercury resistance with integrons and transposon Tn21 was also investigated by PCR. Isolates displayed a high frequency of antimicrobial (erythromycin: 100%; ampicillin: 85%; trimethoprim: 78%) and heavy metal (Zn2+: 95%; Cd2+: 91%) resistance. No correlation was established between heavy metal and multiple antibiotic resistance indices. Significant positive correlations were observed between heavy metal resistance profiles, indices, Cu2+ and Cr3+ resistance with erythromycin resistance. Significant positive correlations were observed between merA (24%)/Tn21 (24%) presence and heavy metal resistance profiles and indices; however, significant negative correlations were obtained between integron-associated qacE∆1 (43%) and sulI (26%) gene presence and heavy metal resistance indices. Heavy metal and antimicrobial agents co-select for resistance, with fish-associated, resistant bacteria demonstrating simultaneous heavy metal resistance. Thus, care should be taken when using anti-fouling heavy metals as feed additives in aquaculture facilities.

PD Lab

Directory of Open Access Journals (Sweden)

Marcel Bilow

2015-08-01

Full Text Available PD Lab explores the applications of building sector related product development.  PD lab investigates and tests digital production technologies like CNC milled wood connections. It will also act as a platform in its wider meaning to investigate the effects and influences of file to factory production, to explore the potential in the field of sustainability, material use, logistics and the interaction of stakeholders within the chain of the building process.

MatLab Script and Functional Programming

Science.gov (United States)

Shaykhian, Gholam Ali

2007-01-01

MatLab Script and Functional Programming: MatLab is one of the most widely used very high level programming languages for scientific and engineering computations. It is very user-friendly and needs practically no formal programming knowledge. Presented here are MatLab programming aspects and not just the MatLab commands for scientists and engineers who do not have formal programming training and also have no significant time to spare for learning programming to solve their real world problems. Specifically provided are programs for visualization. The MatLab seminar covers the functional and script programming aspect of MatLab language. Specific expectations are: a) Recognize MatLab commands, script and function. b) Create, and run a MatLab function. c) Read, recognize, and describe MatLab syntax. d) Recognize decisions, loops and matrix operators. e) Evaluate scope among multiple files, and multiple functions within a file. f) Declare, define and use scalar variables, vectors and matrices.

Exploring linear algebra labs and projects with Mathematica

CERN Document Server

Arangala, Crista

2014-01-01

Matrix Operations Lab 0: An Introduction to Mathematica Lab 1: Matrix Basics and Operations Lab 2: A Matrix Representation of Linear Systems Lab 3: Powers, Inverses, and Special Matrices Lab 4: Graph Theory and Adjacency Matrices Lab 5: Permutations and Determinants Lab 6: 4 x 4 Determinants and Beyond Project Set 1 Invertibility Lab 7: Singular or Nonsingular? Why Singularity Matters Lab 8: Mod It Out, Matrices with Entries in ZpLab 9: It's a Complex World Lab 10: Declaring Independence: Is It Linear? Project Set 2 Vector Spaces Lab 11: Vector Spaces and SubspacesLab 12: Basing It All on Just a Few Vectors Lab 13: Linear Transformations Lab 14: Eigenvalues and Eigenspaces Lab 15: Markov Chains, An Application of Eigenvalues Project Set 3 Orthogonality Lab 16: Inner Product Spaces Lab 17: The Geometry of Vector and Inner Product SpacesLab 18: Orthogonal Matrices, QR Decomposition, and Least Squares Regression Lab 19: Symmetric Matrices and Quadratic Forms Project Set 4 Matrix Decomposition with Applications L...

Effect of sputtered lanthanum hexaboride film thickness on field emission from metallic knife edge cathodes

Science.gov (United States)

Kirley, M. P.; Novakovic, B.; Sule, N.; Weber, M. J.; Knezevic, I.; Booske, J. H.

2012-03-01

We report experiments and analysis of field emission from metallic knife-edge cathodes, which are sputter-coated with thin films of lanthanum hexaboride (LaB6), a low-work function material. The emission current is found to depend sensitively on the thickness of the LaB6 layer. We find that films thinner than 10 nm greatly enhance the emitted current. However, cathodes coated with a thicker layer of LaB6 are observed to emit less current than the uncoated metallic cathode. This result is unexpected due to the higher work function of the bare metal cathode. We show, based on numerical calculation of the electrostatic potential throughout the structure, that the external (LaB6/vacuum) barrier is reduced with respect to uncoated samples for both thin and thick coatings. However, this behavior is not exhibited at the internal (metal/LaB6) barrier. In thinly coated samples, electrons tunnel efficiently through both the internal and external barrier, resulting in current enhancement with respect to the uncoated case. In contrast, the thick internal barrier in thickly coated samples suppresses current below the value for uncoated samples in spite of the lowered external barrier. We argue that this coating thickness variation stems from a relatively low (no higher than 1018 cm-3) free carrier density in the sputtered polycrystalline LaB6.

Clinical relevance of determination the changes of serum cTnI, NPY and PGMP contents after treatment in patients with coronary heart diseases

International Nuclear Information System (INIS)

Ge Hengsong; Ma Chao; Wu Kaixia

2011-01-01

Objective: To explore the clinical significance of changes of serum cTnI, NPY and PGMP levels in patients with coronary heart diseases after treatment. Methods: Serum NPY, PGMP (with RIA), serum cTnI (with Biochemistry) levels were measured in 41 patients with coronary heart diseases both before and after treatment as well as in 35 normal controls. Results: Before treatment, serum cTnI, NPY and PGMP levels were significantly higher than those in controls (P 0.05), but serum PGMP level remained prominently higher (P<0.05). Conclusion: Pathogenesis and progress of CHD, might be closely related to serum cTnI, NPY and PGMP levels. (authors)

Improving the Quality of Lab Reports by Using Them as Lab Instructions

Science.gov (United States)

Haagen-Schuetzenhoefer, Claudia

2012-10-01

Lab exercises are quite popular in teaching science. Teachers have numerous goals in mind when teaching science laboratories. Nevertheless, empirical research draws a heterogeneous picture of the benefits of lab work. Research has shown that it does not necessarily contribute to the enhancement of practical abilities or content knowledge. Lab activities are frequently based on recipe-like, step-by-step instructions ("cookbook style"), which do not motivate students to engage cognitively. Consequently, students put the emphasis on "task completion" or "manipulating equipment."2

Comparison of Ultrasonic Welding and Thermal Bonding for the Integration of Thin Film Metal Electrodes in Injection Molded Polymeric Lab-on-Chip Systems for Electrochemistry

Directory of Open Access Journals (Sweden)

Marco Matteucci

2016-10-01

Full Text Available We compare ultrasonic welding (UW and thermal bonding (TB for the integration of embedded thin-film gold electrodes for electrochemical applications in injection molded (IM microfluidic chips. The UW bonded chips showed a significantly superior electrochemical performance compared to the ones obtained using TB. Parameters such as metal thickness of electrodes, depth of electrode embedding, delivered power, and height of energy directors (for UW, as well as pressure and temperature (for TB, were systematically studied to evaluate the two bonding methods and requirements for optimal electrochemical performance. The presented technology is intended for easy and effective integration of polymeric Lab-on-Chip systems to encourage their use in research, commercialization and education.

Reforming Cookbook Labs

Science.gov (United States)

Peters, Erin

2005-01-01

Deconstructing cookbook labs to require the students to be more thoughtful could break down perceived teacher barriers to inquiry learning. Simple steps that remove or disrupt the direct transfer of step-by-step procedures in cookbook labs make students think more critically about their process. Through trials in the author's middle school…

The Portuguese Contribution for lab2go - pt.lab2go

Directory of Open Access Journals (Sweden)

Maria Teresa Restivo

2013-01-01

Full Text Available Online experimentation provides innovative and valuable tools for use in academy, in high schools, in industry and in medical areas. It has also become a precious tool for educational and training purposes in any of those areas. Looking at online experimentation as a pure distance learning tool it represents a very efficient way of sharing hands-on capabilities, for example with developing countries. In Portugal a new consortium of online experimentation was created for fostering the national potential, using the Portuguese version of lab2go web platform, pt.lab2go. The authors pretend to demonstrate some of capabilities of the consortium in sharing online labs.

In-house preparation of lectin panel and detection of Tn polyagglutination

Directory of Open Access Journals (Sweden)

Sudipta Sekhar Das

2015-01-01

Full Text Available Polyagglutination is a condition in which red cells are agglutinated by ABO-compatible adult human sera, but not by cord blood sera and may be acquired or inherited. Lectins are invaluable reagents in the investigation of red cells polyagglutination. We prepared in-house lectin panel and confirmed Tn polyagglutination in a pregnant lady. The lady was anemic and refused blood transfusion elsewhere due to serological discrepancy. We found ABO discrepancy and an incompatible minor cross-match in the initial investigation and suspected polyagglutination. Confirmation of polyagglutination was done using adult and cord sera. We then used the in-house lectin panels to detect the type of polyagglutination. The agglutination pattern with the various lectins was suggestive of Tn polyagglutination, which was further supported by the enzyme study. Most blood banks in India lack commercial lectin panels because of cost and procurement difficulty. Lectins play an important role in the diagnosis and differentiation of polyagglutination and immunohematological management of patient. The important and basic lectins can be prepared in-house using specific raw seeds following standardized protocol.

In-house preparation of lectin panel and detection of Tn polyagglutination.

Science.gov (United States)

Das, Sudipta Sekhar

2015-01-01

Polyagglutination is a condition in which red cells are agglutinated by ABO-compatible adult human sera, but not by cord blood sera and may be acquired or inherited. Lectins are invaluable reagents in the investigation of red cells polyagglutination. We prepared in-house lectin panel and confirmed Tn polyagglutination in a pregnant lady. The lady was anemic and refused blood transfusion elsewhere due to serological discrepancy. We found ABO discrepancy and an incompatible minor cross-match in the initial investigation and suspected polyagglutination. Confirmation of polyagglutination was done using adult and cord sera. We then used the in-house lectin panels to detect the type of polyagglutination. The agglutination pattern with the various lectins was suggestive of Tn polyagglutination, which was further supported by the enzyme study. Most blood banks in India lack commercial lectin panels because of cost and procurement difficulty. Lectins play an important role in the diagnosis and differentiation of polyagglutination and immunohematological management of patient. The important and basic lectins can be prepared in-house using specific raw seeds following standardized protocol.

LabVIEW Support at CERN

CERN Multimedia

HR Department

2010-01-01

Since the beginning of 2009, due to the CERN restructuring, LabVIEW support moved from the IT to the EN department, joining the Industrial Controls and Electronics Group (ICE). LabVIEW support has been merged with the Measurement, Test and Analysis (MTA) section which, using LabVIEW, has developed most of the measurement systems to qualify the LHC magnets and components over the past 10 years. The post mortem analysis for the LHC hardware commissioning has also been fully implemented using LabVIEW, customised into a framework, called RADE, for CERN needs. The MTA section has started with a proactive approach sharing its tools and experience with the CERN LabVIEW community. Its framework (RADE) for CERN integrated application development has been made available to the users. Courses on RADE have been integrated into the standard National Instruments training program at CERN. RADE and LabVIEW support were merged together in 2010 on a single email address:labview.support@cern.ch For more information please...

Size effect of added LaB6 particles on optical properties of LaB6/Polymer composites

International Nuclear Information System (INIS)

Yuan Yifei; Zhang Lin; Hu Lijie; Wang Wei; Min Guanghui

2011-01-01

Modified LaB 6 particles with sizes ranging from 50 nm to 400 nm were added into polymethyl methacrylate (PMMA) matrix in order to investigate the effect of added LaB 6 particles on optical properties of LaB 6 /PMMA composites. Method of in-situ polymerization was applied to prepare PMMA from raw material—methyl methacrylate (MMA), a process during which LaB 6 particles were dispersed in MMA. Ultraviolet–visible–near infrared (UV–vis–NIR) absorption spectrum was used to study optical properties of the as-prepared materials. The difference in particle size could apparently affect the composites' absorption of visible light around wavelength of 600 nm. Added LaB 6 particles with size of about 70 nm resulted in the best optical properties among these groups of composites. - Graphical abstract: 70 nm LaB 6 particles resulted in the best performance on absorption of VIS and NIR, which could not be apparently achieved by LaB 6 particles beyond nano-scale. Highlights: ► LaB 6 /PMMA composites were prepared using the method of in-situ polymerization. ► LaB 6 particles added in MMA prolonged the time needed for its pre-polymerization. ► Nanosized LaB 6 particles could obviously absorb much NIR but little VIS.

Assessing Tn5 and Sleeping Beauty for transpositional transgenesis by cytoplasmic injection into bovine and ovine zygotes

Science.gov (United States)

Bevacqua, R. J.; Fernandez-Martin, R.; Canel, N. G.; Gibbons, A.; Texeira, D.; Lange, F.; Vans Landschoot, G.; Savy, V.; Briski, O.; Hiriart, M. I.; Grueso, E.; Ivics, Z.; Taboga, O.; Kues, W. A.; Ferraris, S.

2017-01-01

Transgenic domestic animals represent an alternative to bioreactors for large-scale production of biopharmaceuticals and could also provide more accurate biomedical models than rodents. However, their generation remains inefficient. Recently, DNA transposons allowed improved transgenesis efficiencies in mice and pigs. In this work, Tn5 and Sleeping Beauty (SB) transposon systems were evaluated for transgenesis by simple cytoplasmic injection in livestock zygotes. In the case of Tn5, the transposome complex of transposon nucleic acid and Tn5 protein was injected. In the case of SB, the supercoiled plasmids encoding a transposon and the SB transposase were co-injected. In vitro produced bovine zygotes were used to establish the cytoplasmic injection conditions. The in vitro cultured blastocysts were evaluated for reporter gene expression and genotyped. Subsequently, both transposon systems were injected in seasonally available ovine zygotes, employing transposons carrying the recombinant human factor IX driven by the beta-lactoglobulin promoter. The Tn5 approach did not result in transgenic lambs. In contrast, the Sleeping Beauty injection resulted in 2 lambs (29%) carrying the transgene. Both animals exhibited cellular mosaicism of the transgene. The extraembryonic tissues (placenta or umbilical cord) of three additional animals were also transgenic. These results show that transpositional transgenesis by cytoplasmic injection of SB transposon components can be applied for the production of transgenic lambs of pharmaceutical interest. PMID:28301581

TELECOM LAB

CERN Multimedia

IT-CS-TEL Section

2001-01-01

The Telecom Lab is moving from Building 104 to Building 31 S-026, with its entrance via the ramp on the side facing Restaurant n°2. The help desk will thus be closed to users on Tuesday 8 May. On May 9, the Lab will only be able to deal with problems of a technical nature at the new address and it will not be able to process any new subscription requests throughout the week from 7 to 11 May. We apologise for any inconvenience this may cause and thank you for your understanding.

Performances of TN {sup registered} 24 E. An AREVA used fuel transport and interim storage cask for the German market

Energy Technology Data Exchange (ETDEWEB)

Brion, Thomas [AREVA TN International, Montigny Le Bretonneux (France)

2013-07-01

Part of the AREVA Group, TN International offers a complete range of transport and interim storage solutions for radioactive materials throughout the entire nuclear fuel cycle. A world leader in its sector, TN International has supported for 50 years the expansion of the nuclear industry, in particular by providing expertise in secure packing systems for the storage of used fuel assemblies. As an answer to EON and EnBW, two German utilities, needs, TN International has designed and manufactured the TN {sup registered} 24E cask, offering the following high level performances: 1. transport and storage over a period of 40 years of up to 21 PWR spent nuclear fuel (SNF), allowing for example to load up to 17 MOX fuel assemblies and 4 UOX SNF. 2. high flexibility in the fuel assemblies loading plans, inducing no general predefined constraints with regards to the MOX or UOX fuel positions in the basket of the cask Safety margin related to radioprotection, thermal and mechanical behaviour of the fuel assemblies can be calculated loading plan per loading plan. (orig.)

Detección del antígeno Tn en tumores epiteliales con la lectina de Vicia villosa isolectina B4.

Directory of Open Access Journals (Sweden)

Catalina Limpias

2010-10-01

Full Text Available Antecedentes. Los epítopes T, Tn y sTn, se expresan en un alto porcentaje de tumores epiteliales y pueden detectarse con anticuerpos monoclonales y lectinas. Objetivo. Evaluar diferencias de expresión del antígeno Tn en cortes histológicos de epitelios no neoplásicos y tumores epiteliales mediante isolectina B4 de Vicia villosa. Material y métodos. Se evaluaron semicuantitativamente localización, intensidad y porcentaje de expresión del antígeno en carcinomas in-situ e infiltrantes y epitelios no neoplásicos de cérvix, seno y urotelio, mediante isolectina B4. Resultados La expresión de Tn en cérvix predominó en membrana de células no neoplásicas y citoplasma de células tumorales; su intensidad fue mayor en carcinomas in-situ e infiltrantes comparado con epitelio no neoplásico aunque en este el porcentaje de expresión fue mayor. En seno, la expresión de Tn fue predominantemente citoplasmática con intensidad similar, el porcentaje de expresión fué mayor en carcinomas ductales in-situ e infiltrantes. En urotelio no neoplásico y tumoral la expresión de Tn predominó en citoplasma; la intensidad y el porcentaje de expresión fueron mayores en neoplasias no invasivas de bajo y alto grado, mientras que en urotelio no neoplásico fue baja y no hubo tendencia definida en tumores infiltrantes. Conclusiones. La detección del antígeno Tn mediante la lectina VVB4 mostró una mayor extensión de marcación en carcinomas ductales de seno en relación con el epitelio no neoplásico, pero no mostró una tendencia definida entre el tejido normal, ni diferentes etapas del desarrollo de los tumores de cérvix y urotelio. Estos hallazgos pueden atribuirse a la heterogeneidad de los procesos carcinogénicos o a que la especificidad de la lectina VVB4 no está restringida a este antígeno.

75 FR 69398 - Foreign-Trade Zone 78-Nashville, TN; Application for Expansion

Science.gov (United States)

2010-11-12

... Company, 1800 Zinc Plant Road, Clarksville; and Proposed Site 12 (451.0 acres)--Gallatin Industrial Center..., TN; Application for Expansion An application has been submitted to the Foreign-Trade Zones Board (the... application was submitted pursuant to the provisions of the Foreign- Trade Zones Act, as amended (19 U.S.C...

Integrative Gene Cloning and Expression System for Streptomyces sp. US 24 and Streptomyces sp. TN 58 Bioactive Molecule Producing Strains

Directory of Open Access Journals (Sweden)

Samiha Sioud

2009-01-01

Full Text Available Streptomyces sp. US 24 and Streptomyces sp. TN 58, two strains producing interesting bioactive molecules, were successfully transformed using E. coli ET12567 (pUZ8002, as a conjugal donor, carrying the integrative plasmid pSET152. For the Streptomyces sp. US 24 strain, two copies of this plasmid were tandemly integrated in the chromosome, whereas for Streptomyces sp. TN 58, the integration was in single copy at the attB site. Plasmid pSET152 was inherited every time for all analysed Streptomyces sp. US 24 and Streptomyces sp. TN 58 exconjugants under nonselective conditions. The growth, morphological differentiation, and active molecules production of all studied pSET152 integrated exconjugants were identical to those of wild type strains. Consequently, conjugal transfer using pSET152 integration system is a suitable means of genes transfer and expression for both studied strains. To validate the above gene transfer system, the glucose isomerase gene (xylA from Streptomyces sp. SK was expressed in strain Streptomyces sp. TN 58. Obtained results indicated that heterologous glucose isomerase could be expressed and folded effectively. Glucose isomerase activity of the constructed TN 58 recombinant strain is of about eighteenfold higher than that of the Streptomyces sp. SK strain. Such results are certainly of importance due to the potential use of improved strains in biotechnological process for the production of high-fructose syrup from starch.

Novel Tn916-like elements confer aminoglycoside/macrolide co-resistance in clinical isolates of Streptococcus gallolyticus ssp. gallolyticus.

Science.gov (United States)

Kambarev, Stanimir; Pecorari, Frédéric; Corvec, Stéphane

2018-02-09

Streptococcus gallolyticus ssp. gallolyticus (Sgg) is a commensal bacterium and an opportunistic pathogen. In humans it has been clinically associated with the incidence of colorectal cancer (CRC) and epidemiologically recognized as an emerging cause of infective endocarditis (IE). The standard therapy of Sgg includes the administration of a penicillin in combination with an aminoglycoside. Even though penicillin-resistant isolates have still not been reported, epidemiological studies have shown that this microbe is a reservoir of multiple acquired genes, conferring resistance to tetracyclines, aminoglycosides, macrolides and glycopeptides. However, the underlying antibiotic resistance mobilome of Sgg remains poorly understood. To investigate the mobile genetic basis of antibiotic resistance in multiresistant clinical Sgg. Isolate NTS31106099 was recovered from a patient with IE and CRC at Nantes University Hospital, France and studied by Illumina WGS and comparative genomics. Molecular epidemiology of the identified mobile element(s) was performed using antibiotic susceptibility testing (AST), PCR, PFGE and WGS. Mobility was investigated by PCR and filter mating. Two novel conjugative transposons, Tn6263 and Tn6331, confer aminoglycoside/macrolide co-resistance in clinical Sgg. They display classical family Tn916/Tn1545 modular architecture and harbour an aph(3')-III→sat4→ant(6)-Ia→erm(B) multiresistance gene cluster, related to pRE25 of Enterococcus faecium. These and/or closely related elements are highly prevalent among genetically heterogeneous clinical isolates of Sgg. Previously unknown Tn916-like mobile genetic elements conferring aminoglycoside/macrolide co-resistance make Sgg, collectively with other gut Firmicutes such as enterococci and eubacteria, a potential laterally active reservoir of these antibiotic resistance determinants among the mammalian gastrointestinal microbiota. © The Author(s) 2018. Published by Oxford University Press on behalf

Chromosomal insertion of the entire Escherichia coli lactose operon, into two strains of Pseudomonas, using a modified mini-Tn5 delivery system

DEFF Research Database (Denmark)

Hansen, L. H.; Sørensen, S. J.; Jensen, Lars Bogø

1997-01-01

A 12-kb PstI fragment including the entire E. coli lactose operon (lacIPOZYA) was inserted in one copy into the chromosome of Pseudomonas putida, Pseudomonas fluorescens and an E. coli strain with lac(-) phenotype. This was made possible by improvements of an already existing mini-Tn5 transposon...... flanked by NotI sites needed in the mini-Tn5 delivery system; (b) the generation of E. coli nonlysogenic strains expressing the pi protein thus being capable of maintaining and delivering R6K-based mini-Tn5 vectors to other E. coli strains; (c) the successful insertion of the E. coli lactose operon...... into the P. fluorescens chromosome giving P. fluorescens the ability to grow on lactose; (d) evidence from Southern blotting that contradicts the assumption that the mini-Tn5 delivery system always creates one-copy inserts. These improvements allow insertion of large DNA fragments encoding highly expressed...

Ab initio calculations of the electronic structure and bonding characteristics of LaB6

International Nuclear Information System (INIS)

Hossain, Faruque M.; Riley, Daniel P.; Murch, Graeme E.

2005-01-01

Lanthanum hexaboride (LaB 6 , NIST SRM-660a) is widely used as a standard reference material for calibrating the line position and line shape parameters of powder diffraction instruments. The accuracy of this calibration technique is highly dependent on how completely the reference material is characterized. Critical to x-ray diffraction, this understanding must include the valence of the La atomic position, which in turn will influence the x-ray form factor (f) and hence the diffracted intensities. The electronic structure and bonding properties of LaB 6 have been investigated using ab initio plane-wave pseudopotential total energy calculations. The electronic properties and atomic bonding characteristics were analyzed by estimating the energy band structure and the density of states around the Fermi energy level. The calculated energy band structure is consistent with previously reported experimental findings; de Haas-van Alphen and two-dimensional angular correlation of electron-positron annihilation radiation. In addition, the bond strengths and types of atomic bonds in the LaB 6 compound were estimated by analyzing the Mulliken charge density population. The calculated result revealed the coexistence of covalent, ionic, and metallic bonding in the LaB 6 system and partially explains its high efficiency as a thermionic emitter

Thin film Ag superlens towards lab-on-a-chip integration

DEFF Research Database (Denmark)

Jeppesen, Claus; Nielsen, Rasmus Bundgaard; Boltasseva, Alexandra

2009-01-01

A thin metal film near-field superlens, as originally suggested by Pendry and realized by Fang et al. and Melville et al., is investigated with emphasis on materials suitable for integration on a lab-on-a-chip platform. A chemically resistant cyclo-olefin copolymer (COC), mr-I-T85 from microresist...... technology, is applied as dielectric matrix/spacer for an Ag thin film superlens. The superlens successfully resolves 80 nm half-pitch gratings when illuminated with UV radiation at a free space wavelength of 365 nm. The superlens design, fabrication and characterization is discussed....

Payments to the Lab

Science.gov (United States)

Goals Recycling Green Purchasing Pollution Prevention Reusing Water Resources Environmental Management the Lab Make payments for event registrations, sponsorships, insurance, travel, other fees. Contact Treasury Team (505) 667-4090 Email If you need to make a payment to the Lab for an event registration

ScalaLab and GroovyLab: Comparing Scala and Groovy for Scientific Computing

Directory of Open Access Journals (Sweden)

Stergios Papadimitriou

2015-01-01

Full Text Available ScalaLab and GroovyLab are both MATLAB-like environments for the Java Virtual Machine. ScalaLab is based on the Scala programming language and GroovyLab is based on the Groovy programming language. They present similar user interfaces and functionality to the user. They also share the same set of Java scientific libraries and of native code libraries. From the programmer's point of view though, they have significant differences. This paper compares some aspects of the two environments and highlights some of the strengths and weaknesses of Scala versus Groovy for scientific computing. The discussion also examines some aspects of the dilemma of using dynamic typing versus static typing for scientific programming. The performance of the Java platform is continuously improved at a fast pace. Today Java can effectively support demanding high-performance computing and scales well on multicore platforms. Thus, both systems can challenge the performance of the traditional C/C++/Fortran scientific code with an easier to use and more productive programming environment.

CDC Lab Values

Centers for Disease Control (CDC) Podcasts

More than fifteen hundred scientists fill the lab benches at CDC, logging more than four million hours each year. CDC’s laboratories play a critical role in the agency’s ability to find, stop, and prevent disease outbreaks. This podcast provides a brief overview of what goes on inside CDC’s labs, and why this work makes a difference in American’s health.

Tele-Lab IT-Security: an Architecture for an online virtual IT Security Lab

Directory of Open Access Journals (Sweden)

Christoph Meinel

2008-05-01

Full Text Available Recently, Awareness Creation in terms of IT security has become a big thing – not only for enterprises. Campaigns for pupils try to highlight the importance of IT security even in the user’s early years. Common practices in security education – as seen in computer science courses at universities – mainly consist of literature and lecturing. In the best case, the teaching facility offers practical courses in a dedicated isolated computer lab. Additionally, there are some more or less interactive e-learning applications around. Most existing offers can do nothing more than impart theoretical knowledge or basic information. They all lack of possibilities to provide practical experience with security software or even hacker tools in a realistic environment. The only exceptions are the expensive and hard-to-maintain dedicated computer security labs. Those can only be provided by very few organizations. Tele-Lab IT-Security was designed to offer hands-on experience exercises in IT security without the need of additional hardware or maintenance expenses. The existing implementation of Tele-Lab even provides access to the learning environment over the Internet – and thus can be used anytime and anywhere. The present paper describes the extended architecture on which the current version of the Tele-Lab server is built.

Guidelines for Urban Labs

DEFF Research Database (Denmark)

Scholl, Christian; Agger Eriksen, Mette; Baerten, Nik

2017-01-01

urban lab initiatives from five different European cities: Antwerp (B), Graz and Leoben (A), Maastricht (NL) and Malmö (S). We do not pretend that these guidelines touch upon all possible challenges an urban lab may be confronted with, but we have incorporated all those we encountered in our...

Expressing a bacterial mercuric ion binding protein in plant for phytoremediation of heavy metals.

Science.gov (United States)

Hsieh, Ju-Liang; Chen, Ching-Yi; Chiu, Meng-Hsuen; Chein, Mei-Fang; Chang, Jo-Shu; Endo, Ginro; Huang, Chieh-Chen

2009-01-30

A specific mercuric ion binding protein (MerP) originating from transposon TnMERI1 of Bacillus megaterium strain MB1 isolated from Minamata Bay displayed good adsorption capability for a variety of heavy metals. In this study, the Gram-positive MerP protein was expressed in transgenic Arabidopsis to create a model system for phytoremediation of heavy metals. Under control of an actin promoter, the transgenic Arabidpsis showed higher tolerance and accumulation capacity for mercury, cadium and lead when compared with the control plant. Results from confocal microscopy analysis also indicate that MerP was localized at the cell membrane and vesicles of plant cells. The developed transgenic plants possessing excellent metal-accumulative ability could have potential applications in decontamination of heavy metals.

Applications of Open Source GMAW-Based Metal 3-D Printing

OpenAIRE

Yuenyong Nilsiam; Paul G. Sanders; Joshua M. Pearce

2018-01-01

The metal 3-D printing market is currently dominated by high-end applications, which make it inaccessible for small and medium enterprises, fab labs, and individual makers who are interested in the ability to prototype and additively manufacture final products in metal. Recent progress led to low-cost open-source metal 3-D printers using a gas metal arc welding (GMAW)-based print head. This reduced the cost of metal 3-D printers into the range of desktop prosumer polymer 3-D printers. Consequ...

The characteristics and application of sludge-fly ash ceramic particles (SFCP) as novel filter media

International Nuclear Information System (INIS)

Han Shuxin; Yue Qinyan; Yue Min; Gao Baoyu; Li Qian; Yu Hui; Zhao Yaqin; Qi Yuanfeng

2009-01-01

Novel filter media-sludge-fly ash ceramic particles (SFCP) were prepared using dewatered sludge, fly ash and clay with a mass ratio of 1:1:1. Compared with commercial ceramic particles (CCP), SFCP had higher total porosity, larger total surface area and lower bulk and apparent density. Tests of heavy metal elements in lixivium proved that SFCP were safe for wastewater treatment. A lab-scale upflow anaerobic bioreactor was employed to ascertain the application of SFCP in denitrification process using acetate as carbon source. The results showed that SFCP reactor brought a relative superiority to CCP reactor in terms of total nitrogen (TN) removal at the optimum C/N ratio of 4.03 when volumetric loading rates (VLR) ranged from 0.33 to 3.69 kg TN (m 3 d) -1 . Therefore, SFCP application, as a novel process of treating wastes with wastes, provided a promising way in sludge and fly ash utilization.

Design analysis report for the TN-WHC cask and transportation system

Energy Technology Data Exchange (ETDEWEB)

Brisbin, S.A., Fluor Daniel Hanford

1997-02-13

This document presents the evaluation of the Spent Nuclear Fuel Cask and Transportation System. The system design was developed by Transnuclear, Inc. and its team members NAC International, Nelson Manufacturing, Precision Components Corporation, and Numatec, Inc. The cask is designated the TN-WHC cask. This report describes the design features and presents preliminary analyses performed to size critical dimensions of the system while meeting the requirements of the performance specification.

GeneLab: Open Science For Exploration

Science.gov (United States)

Galazka, Jonathan

2018-01-01

The NASA GeneLab project capitalizes on multi-omic technologies to maximize the return on spaceflight experiments. The GeneLab project houses spaceflight and spaceflight-relevant multi-omics data in a publicly accessible data commons, and collaborates with NASA-funded principal investigators to maximize the omics data from spaceflight and spaceflight-relevant experiments. I will discuss the current status of GeneLab and give specific examples of how the GeneLab data system has been used to gain insight into how biology responds to spaceflight conditions.

Ntal/Lab/Lat2

DEFF Research Database (Denmark)

Iwaki, Shoko; Jensen, Bettina M; Gilfillan, Alasdair M

2007-01-01

T cells. As demonstrated in monocytes and B cells, phosphorylated NTAL/LAB/LAT2 recruits signaling molecules such as Grb2, Gab1 and c-Cbl into receptor-signaling complexes. Although gene knock out and knock down studies have indicated that NTAL/LAB/LAT2 may function as both a positive and negative...

Application of the dynamic characterization of metals in automotive industry

Science.gov (United States)

D'Aiuto, Fabio; De Caro, Daniele; Federici, Claudio; Tedesco, Michele M.; Ziggiotti, Alessandro; Cadoni, Ezio

2015-09-01

This paper presents the experimental methodology used by R&D EMEA - Global Materials Labs Department to test metals at high strain rate of 500 s-1. The implementation of dynamic results in commercial FEM Software LS - DYNA for crash simulation are presented. The effects of the strain rate on the tensile properties of metals, used in automotive field, are evaluated using results obtained from a direct tension split Hopkinson bar, built in collaboration with the University of Applied Sciences of Southern Switzerland DynaMat Lab. Finally the complete mechanical characterization of the Magnesium alloy AZ31B is presented, from static up to dynamic tests, showing its applications in FCA (Fiat Chrysler Automobiles), problems and future developments.

OpenLabNotes--An Electronic Laboratory Notebook Extension for OpenLabFramework.

Science.gov (United States)

List, Markus; Franz, Michael; Tan, Qihua; Mollenhauer, Jan; Baumbach, Jan

2015-10-06

Electronic laboratory notebooks (ELNs) are more accessible and reliable than their paper based alternatives and thus find widespread adoption. While a large number of commercial products is available, small- to mid-sized laboratories can often not afford the costs or are concerned about the longevity of the providers. Turning towards free alternatives, however, raises questions about data protection, which are not sufficiently addressed by available solutions. To serve as legal documents, ELNs must prevent scientific fraud through technical means such as digital signatures. It would also be advantageous if an ELN was integrated with a laboratory information management system to allow for a comprehensive documentation of experimental work including the location of samples that were used in a particular experiment. Here, we present OpenLabNotes, which adds state-of-the-art ELN capabilities to OpenLabFramework, a powerful and flexible laboratory information management system. In contrast to comparable solutions, it allows to protect the intellectual property of its users by offering data protection with digital signatures. OpenLabNotes effectively closes the gap between research documentation and sample management, thus making Open-LabFramework more attractive for laboratories that seek to increase productivity through electronic data management.

OpenLabNotes - An Electronic Laboratory Notebook Extension for OpenLabFramework.

Science.gov (United States)

List, Markus; Franz, Michael; Tan, Qihua; Mollenhauer, Jan; Baumbach, Jan

2015-09-01

Electronic laboratory notebooks (ELNs) are more accessible and reliable than their paper based alternatives and thus find widespread adoption. While a large number of commercial products is available, small- to mid-sized laboratories can often not afford the costs or are concerned about the longevity of the providers. Turning towards free alternatives, however, raises questions about data protection, which are not sufficiently addressed by available solutions. To serve as legal documents, ELNs must prevent scientific fraud through technical means such as digital signatures. It would also be advantageous if an ELN was integrated with a laboratory information management system to allow for a comprehensive documentation of experimental work including the location of samples that were used in a particular experiment. Here, we present OpenLabNotes, which adds state-of-the-art ELN capabilities to OpenLabFramework, a powerful and flexible laboratory information management system. In contrast to comparable solutions, it allows to protect the intellectual property of its users by offering data protection with digital signatures. OpenLabNotes effectively closes the gap between research documentation and sample management, thus making Open- LabFramework more attractive for laboratories that seek to increase productivity through electronic data management.

Altitude Lab

Data.gov (United States)

Federal Laboratory Consortium — The Altitude Lab evaluates the performance of complete oxygen systems operated in individually controlled hypobaric chambers, which duplicate pressures that would be...

GitLab repository management

CERN Document Server

Hethey, Jonathan

2013-01-01

A simple, easy to understand tutorial guide on how to build teams and efficiently use version control, using GitLab.If you are a system administrator in a company that writes software or are in charge of an infrastructure, this book will show you the most important features of GitLab, including how to speed up the overall process

Are Virtual Labs as Effective as Hands-on Labs for Undergraduate Physics? A Comparative Study at Two Major Universities

Science.gov (United States)

Darrah, Marjorie; Humbert, Roxann; Finstein, Jeanne; Simon, Marllin; Hopkins, John

2014-01-01

Most physics professors would agree that the lab experiences students have in introductory physics are central to the learning of the concepts in the course. It is also true that these physics labs require time and money for upkeep, not to mention the hours spent setting up and taking down labs. Virtual physics lab experiences can provide an…

The surface treatment lab team. From left to right : J Carosone, M Malabaila, JP Malivert (front row), M Thiebert and A Lasserre (back row)

CERN Multimedia

2001-01-01

The surface treatment lab covers common metals with rarer ones through a process called electroplating. When electroplating is done, the piece of equipment made out of a common metal (like steel) is submerged in a solution which has the desired rare metal (like gold) dissolved in it. An electric current is then run through the solution to the common metal. The tiny rare metal particles follow the current and stick to the common metal when they reach it. It is then that the plating occcurs. All in all, a relatively simple process, but it can be potentially hazardous.

Enhanced nitrogen removal from piggery wastewater with high NH4+ and low COD/TN ratio in a novel upflow microaerobic biofilm reactor.

Science.gov (United States)

Meng, Jia; Li, Jiuling; Li, Jianzheng; Antwi, Philip; Deng, Kaiwen; Nan, Jun; Xu, Pianpian

2018-02-01

To enhance nutrient removal more cost-efficiently in microaerobic process treating piggery wastewater characterized by high ammonium (NH 4 + -N) and low chemical oxygen demand (COD) to total nitrogen (TN) ratio, a novel upflow microaerobic biofilm reactor (UMBR) was constructed and the efficiency in nutrient removal was evaluated with various influent COD/TN ratios and reflux ratios. The results showed that the biofilm on the carriers had increased the biomass in the UMBR and enhanced the enrichment of slow-growth-rate bacteria such as nitrifiers, denitrifiers and anammox bacteria. The packed bed allowed the microaerobic biofilm process perform well at a low reflux ratio of 35 with a NH 4 + -N and TN removal as high as 93.1% and 89.9%, respectively. Compared with the previously developed upflow microaerobic sludge reactor, the UMBR had not changed the dominant anammox approach to nitrogen removal, but was more cost-efficiently in treating organic wastewater with high NH 4 + -N and low COD/TN ratio. Copyright © 2017 Elsevier Ltd. All rights reserved.

Designing Viable Business Models for Living Labs

Directory of Open Access Journals (Sweden)

Bernhard R. Katzy

2012-09-01

Full Text Available Over 300 regions have integrated the concept of living labs into their economic development strategy since 2006, when the former Finnish Prime Minister Esko Aho launched the living lab innovation policy initiative during his term of European presidency. Despite motivating initial results, however, success cases of turning research into usable new products and services remain few and uncertainty remains on what living labs actually do and contribute. This practitioner-oriented article presents a business excellence model that shows processes of idea creation and team mobilization, new product development, user involvement, and entrepreneurship through which living labs deliver high-potential investment opportunities. Customers of living labs are identified as investors such as venture capitalists or industrial firms because living labs can generate revenue from them to create their own sustainable business model. The article concludes that living labs provide extensive support “lab” infrastructure and that it remains a formidable challenge to finance it, which calls for a more intensive debate.

Advances in piezoelectric thin films for acoustic biosensors, acoustofluidics and lab-on-chip applications

OpenAIRE

Fu, Yong Qing; Luo, Jack; Nguyen, Nam-Trung; Walton, Anthony; Flewitt, Andrew; Zu, Xiao-Tao; Li, Yifan; McHale, Glen; Matthews, Allan; Iborra, Enrique; Du, Hejun; Milne, William

2017-01-01

Recently, piezoelectric thin films including zinc oxide (ZnO) and aluminium nitride (AlN) have found a broad range of lab-on-chip applications such as biosensing, particle/cell concentrating, sorting/patterning, pumping, mixing, nebulisation and jetting. Integrated acoustic wave sensing/microfluidic devices have been fabricated by depositing these piezoelectric films onto a number of substrates such as silicon, ceramics, diamond, quartz, glass, and more recently also polymer, metallic foils a...

RoboLab and virtual environments

Science.gov (United States)

Giarratano, Joseph C.

1994-01-01

A useful adjunct to the manned space station would be a self-contained free-flying laboratory (RoboLab). This laboratory would have a robot operated under telepresence from the space station or ground. Long duration experiments aboard RoboLab could be performed by astronauts or scientists using telepresence to operate equipment and perform experiments. Operating the lab by telepresence would eliminate the need for life support such as food, water and air. The robot would be capable of motion in three dimensions, have binocular vision TV cameras, and two arms with manipulators to simulate hands. The robot would move along a two-dimensional grid and have a rotating, telescoping periscope section for extension in the third dimension. The remote operator would wear a virtual reality type headset to allow the superposition of computer displays over the real-time video of the lab. The operators would wear exoskeleton type arms to facilitate the movement of objects and equipment operation. The combination of video displays, motion, and the exoskeleton arms would provide a high degree of telepresence, especially for novice users such as scientists doing short-term experiments. The RoboLab could be resupplied and samples removed on other space shuttle flights. A self-contained RoboLab module would be designed to fit within the cargo bay of the space shuttle. Different modules could be designed for specific applications, i.e., crystal-growing, medicine, life sciences, chemistry, etc. This paper describes a RoboLab simulation using virtual reality (VR). VR provides an ideal simulation of telepresence before the actual robot and laboratory modules are constructed. The easy simulation of different telepresence designs will produce a highly optimum design before construction rather than the more expensive and time consuming hardware changes afterwards.

The Design:Lab as platform in participatory design research

DEFF Research Database (Denmark)

Binder, Thomas; Brandt, Eva

2008-01-01

The notion of laboratory or simply 'lab' has become popular in recent years in areas outside science and technology development. Learning Labs, Innovation Labs, Usability Labs, Media and Communication Labs and even Art Labs designate institutions or fora dedicated to change and experimentation...... as others have frequently used other metaphors like workshop, studio or atelier in design research. In this article we will argue that the laboratory metaphor is particularly suitable and useful for the design:lab, and we will give examples of how we have worked with the design:lab as a platform...

CDC Lab Values

Centers for Disease Control (CDC) Podcasts

2015-02-02

More than fifteen hundred scientists fill the lab benches at CDC, logging more than four million hours each year. CDC’s laboratories play a critical role in the agency’s ability to find, stop, and prevent disease outbreaks. This podcast provides a brief overview of what goes on inside CDC’s labs, and why this work makes a difference in American’s health.  Created: 2/2/2015 by Office of the Associate Director for Communication (OADC).   Date Released: 2/2/2015.

[Distribution and Pollution Assessment of Nutrient and Heavy Metals in Surface Sediments from Lake Gehu in Southern Jiangsu Province, China].

Science.gov (United States)

Xiong, Chun-hui; Zhagn, Rui-lei; Wu, Xiao-dong; Feng, Li-hui; Wang, Li-qing

2016-03-15

This study investigated the horizontal distribution characteristics of nutrients and heavy metals (Zn, As, Cr, Cu, Ni, Pb, Cd and Hg) in January, 2014, and assessed the potential ecological risk of Lake Gehu. It was found that the average contents of TN and TP were 2,207.94 and 708.62 mg · kg⁻¹ respectively. TN and TP contents of the sediments at the centre were significantly highei than those in the north, while the TN content in the south was also significantly higher than that in the north of Lake Gehu. The average contents of Zn, As, Cr, Cu, Ni, Pb, Cd, Hg were 766.59, 350.66, 307.98, 59.54, 122.67, 168.97, 2.34, 0.41 mg · kg⁻¹, respectively. The content of Cu at the centre was significantly higher than that in the north, and the Zn content at the centre was significantly greater than that in the south of Lake Gehu, however the difference in the content of other heavy metals at these three areas was not significant. Furthermore, the obvious correlation between elements and granularity was only found in the aspect of TP, Cu and Hg. The comprehensive pollution index (PI) indicated that the Lake Gehu was heavily polluted, especially the centre and south areas. The potential ecological risk index (RI) showed that Cd, As and Hg had caused serious pollution in Lake Gehu while the other heavy metals only induced slight or medium pollution. According to the contribution of Cd, As and Hg to RI, it was concluded that the sediments in Lake Gehu were at a serious potential ecological risk.

A new LabVIEW interface for MDSplus

International Nuclear Information System (INIS)

Manduchi, G.; De Marchi, E.; Mandelli, A.

2013-01-01

Highlights: ► Integration object oriented data access layer in LabVIEW. ► A new component of the MDSplus data acquisition package. ► A new approach in the graphical presentation of data acquisition systems. -- Abstract: The paper presents a new interface providing full integration of MDSplus in LabVIEW, based on the recent features of MDSplus, in particular, data streaming, multithreading and Object Oriented interface. Data streaming support fits into the data driven concept of LabVIEW and multithreading is a native concept in LabVIEW. The object oriented interface of MDSplus defines a set of classes which map specific functionality, such as Tree and TreeNode to represent pulse files and data items, respectively, and fits naturally into the LabVIEW Object Oriented programming interface (LVOOP) introduced in version 8.2. MDSplus objects have been mapped onto LabVIEW objects, which act as wrappers to the underlying MDSplus object instance. This approach allows exporting the full MDSplus functionality into LabVIEW retaining the language-independent system view provided by the MDSplus object oriented interface

A new LabVIEW interface for MDSplus

Energy Technology Data Exchange (ETDEWEB)

Manduchi, G., E-mail: gabriele.manduchi@igi.cnr.it [Consorzio RFX, Euratom-ENEA Association, Padova (Italy); De Marchi, E. [Department of Information Engineering, Padova University (Italy); Mandelli, A. [National Instruments (Italy)

2013-10-15

Highlights: ► Integration object oriented data access layer in LabVIEW. ► A new component of the MDSplus data acquisition package. ► A new approach in the graphical presentation of data acquisition systems. -- Abstract: The paper presents a new interface providing full integration of MDSplus in LabVIEW, based on the recent features of MDSplus, in particular, data streaming, multithreading and Object Oriented interface. Data streaming support fits into the data driven concept of LabVIEW and multithreading is a native concept in LabVIEW. The object oriented interface of MDSplus defines a set of classes which map specific functionality, such as Tree and TreeNode to represent pulse files and data items, respectively, and fits naturally into the LabVIEW Object Oriented programming interface (LVOOP) introduced in version 8.2. MDSplus objects have been mapped onto LabVIEW objects, which act as wrappers to the underlying MDSplus object instance. This approach allows exporting the full MDSplus functionality into LabVIEW retaining the language-independent system view provided by the MDSplus object oriented interface.

The chemistry of the liquid alkali metals

International Nuclear Information System (INIS)

Addison, C.C.

1984-01-01

A study of liquid alkali metals. It encourages comparison with molecular solvents in chapter covering the nature and reactivity of dissolved species, solvation, solubility and electrical conductivity of solutions. It demonstrates lab techniques unique to liquid alkali metals. It discusses large-scale applications from storage batteries to sodium-cooled reactors and future fusion reactors, and associated technological problems. Contents: Some Basic Physical and Chemical Properties; Manipulation of the Liquids; The Chemistry of Purification Methods; Species Formed by Dissolved Elements; Solubilities and Analytical Methods; Alkali Metal Mixtures; Solvation in Liquid Metal; Reactions Between Liquid Alkali Metals and Water; Reactions of Nitrogen with Lithium and the Group II Metals in Liquid Sodium; The Formation, Dissociation and Stability of Heteronuclear Polyatomic Anions; Reactions of the Liquid Alkali Metals and Their Alloys with Simple Alipatic Hydrocarbons; Reactions of the Liquid Alkali Metals with Some Halogen Compounds; Hydrogen, Oxygen and Carbon Meters; Surface Chemistry and Wetting; Corrosion of Transition Metals by the Liquid Alkali Metals; Modern Applications of the Liquid Alkali Metals

Dynamic changes of serum TNF-α and cTnI levels in BALB/c Mouse models of autoimmune myocarditis

International Nuclear Information System (INIS)

Han Li'na

2007-01-01

Objective: To prepare mouse models of experimental autoimmune myocarditis (EAM) with subcutaneous injection of synthesized peptide (containing heavy chain segment of cardiac myosin) and examine the dynamic changes of cardiac pathology as well as TNF -α, cTnI and autoimmune antibody contents. Methods: BALB/c mice were subcutaneously injected with 0.8 mg of the synthesized peptide to produce the experimental autoimmune myocarditis (EAM) models. The animals were sacrificed 14, 21, 30 and 60d after establishment of the models, heart muscle was examined pathologically to assess the severity of myocarditis and blood were examined for the plasma contents of TNF-α (with RIA) cTnI (with ELISA) and anti-cardiac myosin heavy chain (CMHC) antibody titier (with ELISA). Results: Evidence of myocardial damage appeared on d14 specimens and progressed there after with interstitial infiltration of macrophages and myocardial necrosis. In the 60d specimens, fibrosis was obvious. The plasma TNF-α and cTnI levels were highest in the d30 specimens and declined in the d60 specimens but still remained much higher than normal. Most of the models demonstrated anti-CMHC antibodies in the 21d and 30d specimens. Conclusion: It is feasible to prepare EMC mouse models with subcutaneous injection of synthesized peptide containing heavy chain segment of cardiac myosin and the plasma levels of TNF-α, cTnI in the models are well correlated to the pathologic change of myocarditis. (authors)

Awakening interest in the natural sciences - BASF's Kids' Labs.

Science.gov (United States)

Lang, Cinthia

2012-01-01

At BASF's Ludwigshafen headquarters, kids and young adults in grades 1-13 can learn about chemistry in the Kids' Labs. Different programs exist for different levels of knowledge. In the two 'Hands-on Lab H(2)O & Co.' Kids' Labs, students from grades 1-6 explore the secrets of chemistry. BASF Kids' Labs have now been set up in over 30 countries. In Switzerland alone, almost 2,000 students have taken part in the 'Water Loves Chemistry' Kids' Lab since it was started in 2011. In Alsace, 600 students have participated to date. In the Teens' Lab 'Xplore Middle School', middle school students explore five different programs with the themes 'substance labyrinth', 'nutrition', 'coffee, caffeine & co.', 'cosmetics' and 'energy'. Biotechnological methods are the focus of the Teens' Lab 'Xplore Biotech' for students taking basic and advanced biology courses. In the 'Xplore High School' Teens' Lab, chemistry teachers present their own experimental lab instruction for students in basic and advanced chemistry courses. The Virtual Lab has been expanding the offerings of the BASF Kids' Labs since 2011. The online lab was developed by the company for the International Year Of Chemistry and gives kids and young adults the opportunity to do interactive experiments outside of the lab.

LabVIEW Real-Time

CERN Multimedia

CERN. Geneva; Flockhart, Ronald Bruce; Seppey, P

2003-01-01

With LabVIEW Real-Time, you can choose from a variety of RT Series hardware. Add a real-time data acquisition component into a larger measurement and automation system or create a single stand-alone real-time solution with data acquisition, signal conditioning, motion control, RS-232, GPIB instrumentation, and Ethernet connectivity. With the various hardware options, you can create a system to meet your precise needs today, while the modularity of the system means you can add to the solution as your system requirements grow. If you are interested in Reliable and Deterministic systems for Measurement and Automation, you will profit from this seminar. Agenda: Real-Time Overview LabVIEW RT Hardware Platforms - Linux on PXI Programming with LabVIEW RT Real-Time Operating Systems concepts Timing Applications Data Transfer

Association of Elevated High Sensitivity Cardiac Troponin T(hs-cTnT Levels with Hemorrhagic Transformation and 3-Month Mortality in Acute Ischemic Stroke Patients with Rheumatic Heart Disease in China.

Directory of Open Access Journals (Sweden)

Junfeng Liu

Full Text Available Elevated levels of high sensitivity cardiac troponin T (hs-cTnT occur in a substantial proportion of patients with acute ischemic stroke (AIS and can predict poor outcome and mortality after stroke. Whether elevated hs-cTnT levels can also predict hemorrhagic transformation (HT or prognosis in AIS patients with rheumatic heart disease (RHD remains unclear.Data from the Chengdu Stroke Registry on consecutive AIS patients with RHD admitted to West China Hospital within 1 month of stroke onset from October 2011 to February 2014 were examined. Clinico-demographic characteristics, HT, functional outcomes and stroke recurrence were compared between patients with elevated hs-cTnT levels (≥14 ng/L and patients with normal hs-cTnT levels (<14 ng/L.The final analysis involved 84 patients (31 males; mean age, 61.6±12.2 years, of whom serum hs-cTnT levels were elevated in 58.3%. Renal impairment was independently associated with elevated hs-cTnT levels (OR 4.184, 95%CI 1.17 to 15.01, P = 0.028, and patients with elevated hs-cTnT levels were at significantly higher risk of HT, 3-month mortality and 3-month disability/mortality (all P≤0.029. After controlling for age, sex, hypertension, renal impairment and National Institutes of Health Stroke Scale score on admission, the risk of HT and 3-month mortality was, respectively, 4.0- and 5.5-fold higher in patients with elevated hs-cTnT levels than in patients with normal hs-cTnT levels.Elevated hs-cTnT levels are independently associated with HT and 3-month mortality in AIS patients with RHD. These results with a small cohort should be verified and extended in large studies.

UniSchooLabs Toolkit: Tools and Methodologies to Support the Adoption of Universities’ Remote and Virtual Labs in Schools

Directory of Open Access Journals (Sweden)

Augusto Chioccariello

2012-11-01

Full Text Available The UniSchooLabs project aims at creating an infrastructure supporting web access to remote/virtual labs and associated educational resources to engage learners with hands-on and minds-on activities in science, technology and math in schools. The UniSchooLabs tool-kit supports the teacher in selecting a remote or virtual lab and developing a lab activity based on an inquiry model template. While working with the toolkit the teacher has access to three main features: a a catalogue of available online laboratories; b an archive of activities created by other users; c a tool for creating new activities or reusing existing ones.

Incorporating lab experience into computer security courses

NARCIS (Netherlands)

Ben Othmane, L.; Bhuse, V.; Lilien, L.T.

2013-01-01

We describe our experience with teaching computer security labs at two different universities. We report on the hardware and software lab setups, summarize lab assignments, present the challenges encountered, and discuss the lessons learned. We agree with and emphasize the viewpoint that security

Lab architecture

Science.gov (United States)

Crease, Robert P.

2008-04-01

There are few more dramatic illustrations of the vicissitudes of laboratory architecturethan the contrast between Building 20 at the Massachusetts Institute of Technology (MIT) and its replacement, the Ray and Maria Stata Center. Building 20 was built hurriedly in 1943 as temporary housing for MIT's famous Rad Lab, the site of wartime radar research, and it remained a productive laboratory space for over half a century. A decade ago it was demolished to make way for the Stata Center, an architecturally striking building designed by Frank Gehry to house MIT's computer science and artificial intelligence labs (above). But in 2004 - just two years after the Stata Center officially opened - the building was criticized for being unsuitable for research and became the subject of still ongoing lawsuits alleging design and construction failures.

mQoL smart lab

DEFF Research Database (Denmark)

De Masi, Alexandre; Ciman, Matteo; Gustarini, Mattia

2016-01-01

serve quality research in all of them. In this paper, we present own "mQoL Smart Lab" for interdisciplinary research efforts on individuals' "Quality of Life" improvement. We present an evolution of our current in-house living lab platform enabling continuous, pervasive data collection from individuals...

Literary Depictions i n Ghazavâtnâmahs That Adress the Crimean War

Directory of Open Access Journals (Sweden)

Kürşat Şamil ŞAHİN

2015-07-01

Full Text Available The Crimean War that started in 1853 between the Ottoman Empire and Russia lasted until 1856. It ended with the defeat of the Russians as England and France sided with the Ottoman Empire. A great number of work has been written then and since about this war which deeply affect our social and politica l life. Among these, there are ghazavâtnâmahs that describe what happened down - to - line, usually by the pen of the poets and writers who closely witnessed the war. The causes of war, the preparations, what happened at the time of expedition and measures tha t were taken, the outcome of the events during the war and afterwards are all brought into sharp relief in most of these works. Whether in verse, prose or mixed typed, these works of art have gradually increased after 15 th century in Turkish literature. Th is genre has decreased by the Ottoman Empire began to decline and the raids were scarce; and it totally disappeared after the tradition of ghaza were ceased. In this study the literary depictions in - the last examples of the genre - Salih Hayri’s Kırım Zafe rnamesi (Hayrâbât, Ahmed Rızâ Trabzonî’s Manzume - i Sivastopol and Süleyman Şâdî’s Muzaffernâme are presented. There are not many studies that focus on literary depictions in ghazavâtnâmahs, particularly on literary war depictions. The characteristics of t hese literary depictions are tried to be explained with reference to ghazavâtnâmahs belonging to the Empire's last era.

Ontology: A Support Structure for a V-Labs Network: Euronet-Lab

Directory of Open Access Journals (Sweden)

Raul Cordeiro Correia

2012-11-01

Full Text Available Our propose is to build a network of virtual laboratories, based in a Virtual Closet that will contain all the elements and parts that are needed to build the various experiences available in a v-labs network (that we call Euronet-Lab. To build this complex network we need to find a system that supports effectively this structure. This probably will be a enormous database of v-labs and independent elements, where will be possible sometimes to “recycle” some of the elements. This means “re-use” the same element several times in many experiences. To do this is necessary to have a structure that allows us to have several instances of the same element. It’s important that in our structure and virtual environment we can create several “images” of the same reality and this images can be used simultaneously in different circuits/experiments. This means that we can create several instances of the same element, to be used in different experiences and exercises.

Future{at}Labs.Prosperity Game{trademark}

Energy Technology Data Exchange (ETDEWEB)

Beck, D.F.; Boyack, K.W.; Berman, M. [Sandia National Labs., Albuquerque, NM (United States). Innovative Alliances Dept.

1996-10-01

Prosperity Games{trademark} are an outgrowth and adaptation of move/countermove and seminar War Games, Prosperity Games{trademark} are simulations that explore complex issues in a variety of areas including economics, politics, sociology, environment, education, and research. These issues can be examined from a variety of perspectives ranging from global, macroeconomic and geopolitical viewpoint down to the details of customer/supplier/market interactions specific industries. All Prosperity Games{trademark} are unique in that both the game format and the player contributions vary from game to game. This report documents the Future{at}Labs.Prosperity Game{trademark} conducted under the sponsorship of the Industry Advisory Boards of the national labs, the national labs, Lockheed Martin Corporation, and the University of California. Players were drawn from all stakeholders involved including government, industry, labs, and academia. The primary objectives of this game were to: (1) explore ways to optimize the role of the multidisciplinary labs in serving national missions and needs; (2) explore ways to increase collaboration and partnerships among government, laboratories, universities, and industry; and (3) create a network of partnership champions to promote findings and policy options. The deliberations and recommendations of these players provided valuable insights as to the views of this diverse group of decision makers concerning the future of the labs.

Efficient transformation of sucrose into high pullulan concentrations by Aureobasidium melanogenum TN1-2 isolated from a natural honey.

Science.gov (United States)

Jiang, Hong; Xue, Si-Jia; Li, Yan-Feng; Liu, Guang-Lei; Chi, Zhen-Ming; Hu, Zhong; Chi, Zhe

2018-08-15

A very high pullulan producing yeast-like fungus, Aureobasidium melanogenum TN1-2 isolated from a natural honey was found to be able to produce 97.0 g/L of pullulan from 140.0 g/L sucrose at a flask level while it could yield 114.0 g/L of pullulan within 134 h during a 10-liter fermentation, the yield was 0.81 g/g and the productivity was 0.86 g/L/h. The high ability to biosynthesize pullulan by this yeast-like fungal strain TN1-2 was related to high glucosyltransferase activity, high phosphofructo-2-kinase activity, high content of its cellular glycerol and low glucose repressor. The Mw of the produced pullulan was 1.42 × 10 5  g/mol. The low Mw may be due to the high α-amylase, glucoamylase and isopullulanase activities. The intracellular level of trehalose had no influence on high pullulan production by the yeast-like fungal strain TN1-2. Copyright © 2018. Published by Elsevier Ltd.

Programming Arduino with LabVIEW

CERN Document Server

Schwartz, Marco

2015-01-01

If you already have some experience with LabVIEW and want to apply your skills to control physical objects and make measurements using the Arduino sensor, this book is for you. Prior knowledge of Arduino and LabVIEW is essential to fully understand the projects detailed in this book.

The L‐type Ca2+ channel facilitates abnormal metabolic activity in the cTnI‐G203S mouse model of hypertrophic cardiomyopathy

Science.gov (United States)

Viola, Helena; Johnstone, Victoria; Cserne Szappanos, Henrietta; Richman, Tara; Tsoutsman, Tatiana; Filipovska, Aleksandra; Semsarian, Christopher

2016-01-01

Key points Genetic mutations in cardiac troponin I (cTnI) are associated with development of hypertrophic cardiomyopathy characterized by myocyte remodelling, disorganization of cytoskeletal proteins and altered energy metabolism.The L‐type Ca2+ channel is the main route for calcium influx and is crucial to cardiac excitation and contraction. The channel also regulates mitochondrial function in the heart by a functional communication between the channel and mitochondria via the cytoskeletal network.We find that L‐type Ca2+ channel kinetics are altered in cTnI‐G203S cardiac myocytes and that activation of the channel causes a significantly greater increase in mitochondrial membrane potential and metabolic activity in cTnI‐G203S cardiac myocytes.These responses occur as a result of impaired communication between the L‐type Ca2+ channel and cytoskeletal protein F‐actin, involving decreased movement of actin–myosin and block of the mitochondrial voltage‐dependent anion channel, resulting in a ‘hypermetabolic’ mitochondrial state.We propose that L‐type Ca2+ channel antagonists, such as diltiazem, might be effective in reducing the cardiomyopathy by normalizing mitochondrial metabolic activity. Abstract Genetic mutations in cardiac troponin I (cTnI) account for 5% of families with hypertrophic cardiomyopathy. Hypertrophic cardiomyopathy is associated with disorganization of cytoskeletal proteins and altered energy metabolism. The L‐type Ca2+ channel (ICa‐L) plays an important role in regulating mitochondrial function. This involves a functional communication between the channel and mitochondria via the cytoskeletal network. We investigate the role of ICa‐L in regulating mitochondrial function in 25‐ to 30‐week‐old cardiomyopathic mice expressing the human disease‐causing mutation Gly203Ser in cTnI (cTnI‐G203S). The inactivation rate of ICa‐L is significantly faster in cTnI‐G203S myocytes [cTnI‐G203S: τ1 = 40.68 ± 3.22, n

Online labs and the MARVEL experience

Directory of Open Access Journals (Sweden)

Dieter Mueller

2005-06-01

Full Text Available MARVEL is a Leonardo da Vinci project that provides a framework to analyse the pedagogic effectiveness of online labs in various heterogeneous areas that include solar energy, robotics, electronics and electro-pneumatics. It is also used as a test bench to compare the implementation of purely remote labs, where all devices are real, versus mixed-reality environments, where real devices work together with simulation models. This paper describes the basic concepts underlying the implementation of such online labs and presents two case studies (which are openly available to the public. A final section discusses the main pedagogical implications of online labs and presents the research directions that are being considered as a follow-up from this project.

OpenLabNotes – An Electronic Laboratory Notebook Extension for OpenLabFramework

Directory of Open Access Journals (Sweden)

List Markus

2015-09-01

Full Text Available Electronic laboratory notebooks (ELNs are more accessible and reliable than their paper based alternatives and thus find widespread adoption. While a large number of commercial products is available, small- to mid-sized laboratories can often not afford the costs or are concerned about the longevity of the providers. Turning towards free alternatives, however, raises questions about data protection, which are not sufficiently addressed by available solutions. To serve as legal documents, ELNs must prevent scientific fraud through technical means such as digital signatures. It would also be advantageous if an ELN was integrated with a laboratory information management system to allow for a comprehensive documentation of experimental work including the location of samples that were used in a particular experiment. Here, we present OpenLabNotes, which adds state-of-the-art ELN capabilities to OpenLabFramework, a powerful and flexible laboratory information management system. In contrast to comparable solutions, it allows to protect the intellectual property of its users by offering data protection with digital signatures. OpenLabNotes effectively closes the gap between research documentation and sample management, thus making Open- LabFramework more attractive for laboratories that seek to increase productivity through electronic data management.

Evaluation of a novel PTFE material for separation and preconcentration of trace levels of metal ions in sequential injection (SI) and sequential injection lab-on-valve (SI-LOV) systems interfaced with detection by ETAAS

DEFF Research Database (Denmark)

Long, Xiangbao; Chomchoei, Roongrat; Gała, Piotr

The operational characteristics of a novel PTFE bead material, granular Algoflon®, used for separation and preconcentration of metal ions via adsorption of on-line generated non-charged metal complexes, were evaluated in a sequential injection (SI) system furnished with an external packed column...... and in a sequential injection lab-on-valve (SI-LOV) system. Employed for the determination of cadmium(II), complexed with diethyldithiophosphate (DDPA), and detection by electrothermal atomic absorption spectrometry (ETAAS), its performance was compared to that of a previously used material, Aldrich PTFE, which had...... demonstrated that PTFE was the most promising for solid-state pretreatments. By comparing the two materials, the Algoflon® beads exhibited much higher sensitivity (1.6107 versus 0.2956 μg l-1 per integrated absorbance (s)), and better retention efficiency (82% versus 74%) and enrichment factor (20.8 versus 17...

Characterizations of purple non-sulfur bacteria isolated from paddy fields, and identification of strains with potential for plant growth-promotion, greenhouse gas mitigation and heavy metal bioremediation.

Science.gov (United States)

Sakpirom, Jakkapan; Kantachote, Duangporn; Nunkaew, Tomorn; Khan, Eakalak

2017-04-01

This study was aimed at selecting purple non-sulfur bacteria (PNSB) isolated from various paddy fields, including Cd- and Zn-contaminated paddy fields, based on their biofertilizer properties. Among 235 PNSB isolates, strain TN110 was most effective in plant growth-promoting substance (PGPS) production, releasing 3.2 mg/L of [Formula: see text] , 4.11 mg/L of 5-aminolevulinic acid (ALA) and 3.62 mg/L of indole-3-acetic acid (IAA), and reducing methane emission up to 80%. This strain had nifH, vnfG and anfG, which are the Mo, V and Fe nitrogenase genes encoded for key enzymes in nitrogen fixation under different conditions. This strain provided 84% and 55% removal of Cd and Zn, respectively. Another isolate, TN414, not only produced PGPS (1.30 mg/L of [Formula: see text] , 0.94 mg/L of ALA and 0.65 mg/L of IAA), but was also efficient in removing both Cd and Zn at 72% and 74%, respectively. Based on 16S rDNA sequencing, strain TN110 was identified as Rhodopseudomonas palustris, while strain TN414 was Rubrivivax gelatinosus. A combination of TN110 and TN414 could potentially provide a biofertilizer, which is a greener alternative to commercial/chemical fertilizers and an agent for bioremediation of heavy metals and greenhouse gas mitigation in paddy fields. Copyright © 2016 Institut Pasteur. All rights reserved.

Tailoring channeled plasmon polaritons in metallic V-grooves

DEFF Research Database (Denmark)

Smith, Cameron; Thilsted, Anil Haraksingh; Marie, Rodolphe

2013-01-01

of propagating plasmons to optimize the trade-off between lateral confinement and loss [2]. Accordingly, the traits of CPPs in metallic V-grooves suggest their widespread implementation, with applications ranging from ultracompact photonic circuitry [3] to lab-on-a-chip sensing. Current CPP research focuses...

Application value of combined measurement of serum sTn, CA242, CA19-9 and CEA in the diagnosis of gastroenterological neoplasm

International Nuclear Information System (INIS)

Zhang Wanzhong; Chen Zhizhou; Fan Zhenfu

2007-01-01

To determine the application value of four serum tumor markers sTn, CA242, CA 19-9 and CEA in the diagnosis of gastroenterological neoplasm, the serum sTn, CA242, CA19-9 and CEA in 30 normal adult controls and 60 patients with gastroenterological neoplasm were measured by IRMA. The results showed that the serum sTn, CA242, CA19-9 and CEA levels in patients with gastric carcinoma or colorectal carcinoma were much higher than those in control group (P<0.01). The serum CEA, CA19-9 and CA242 levels in patients with colorectal carcinoma were significantly higher than those in patients with gastric carcinoma (P<0.01), but the serum sTn level in the former was markedly lower (P<0.01) than that in the latter. The sensitivity of tumor marker increased with the progress of clinical stages, with a considerably higher sensitivity for stage IV compared with stage I-II (P<0.01). The combined test of four tumor markers could be more sensitive than single test in detecting gastric carcinoma and colorectal carcinoma (P<0.05). Four tumor markers are useful for diagnosing gastroenterological neoplasm, and the combined measurement of 4 tumor markers could increase the sensitivity of detecting gastric carcinoma. (authors)

The Telecom Lab is moving

CERN Multimedia

IT Department

2009-01-01

As of 2nd March 2009, the Telecom Lab will move to Building 58 R-017. The Telecom Lab is the central point for all support questions regarding CERN mobile phone services (provision of SIM cards, requests for modifications of subscriptions, diagnostics for mobile phone problems, etc.). The opening hours as well as the contact details for the Telecom Lab remain unchanged: New location: Building 58 R-017 Opening hours: Every week day, from 11 a.m. to 12 a.m. Phone number: 72480 Email address: labo.telecom@cern.ch This change has no impact on support requests for mobile services. Users can still submit their requests concerning mobile phone subscriptions using the usual EDH form (https://edh.cern.ch/Document/GSM). The automatic message sent to inform users of their SIM card availability will be updated to indicate the new Telecom Lab location. You can find all information related to CERN mobile phone services at the following link: http://cern.ch/gsm CS Section - IT/CS group

Towards a Manifesto for Living Lab Co-creation

Science.gov (United States)

Følstad, Asbjørn; Brandtzæg, Petter Bae; Gulliksen, Jan; Börjeson, Mikael; Näkki, Pirjo

There is a growing interest in Living Labs for innovation and development in the field of information and communication technology. In particular there seem to be a tendency that current Living Labs aim to involve users for co-creative purposes. However, the current literature on Living Lab co-creation is severely limited. Therefore an Interact workshop is arranged as a first step towards a manifesto for Living Lab co-creation.

LabVIEW A Developer's Guide to Real World Integration

CERN Document Server

Fairweather, Ian

2011-01-01

LabVIEW(t) has become one of the preeminent platforms for the development of data acquisition and data analysis programs. LabVIEW(t): A Developer's Guide to Real World Integration explains how to integrate LabVIEW into real-life applications. Written by experienced LabVIEW developers and engineers, the book describes how LabVIEW has been pivotal in solving real-world challenges. Each chapter is self-contained and demonstrates the power and simplicity of LabVIEW in various applications, from image processing to solar tracking systems. Many of the chapters explore how exciting new technologies c

Direct reduction of uranium oxide(U3O8) by Li metal and U-metal(Fe, Ni) alloy formation in molten LiCl medium

International Nuclear Information System (INIS)

Cho, Young Hwan; Kim, Tack Jin; Choi, In Kyu; Kim, Won Ho; Jee, Kwang Yong

2004-01-01

Molten salt based electrochemical processes are proposed as a promising method for the future nuclear programs and more specifically for spent fuel processing. The lithium reduction has been introduced to convert actinide oxides into corresponding actinide metal by using lithium metal as a reductant in molten LiCl medium. We have applied similar lab-scale experiments to reduce uranium oxide in an effort to gain additional information on rates and mechanisms

Association of Elevated High Sensitivity Cardiac Troponin T(hs-cTnT) Levels with Hemorrhagic Transformation and 3-Month Mortality in Acute Ischemic Stroke Patients with Rheumatic Heart Disease in China.

Science.gov (United States)

Liu, Junfeng; Wang, Deren; Xiong, Yao; Liu, Bian; Hao, Zilong; Tao, Wendan; Liu, Ming

2016-01-01

Elevated levels of high sensitivity cardiac troponin T (hs-cTnT) occur in a substantial proportion of patients with acute ischemic stroke (AIS) and can predict poor outcome and mortality after stroke. Whether elevated hs-cTnT levels can also predict hemorrhagic transformation (HT) or prognosis in AIS patients with rheumatic heart disease (RHD) remains unclear. Data from the Chengdu Stroke Registry on consecutive AIS patients with RHD admitted to West China Hospital within 1 month of stroke onset from October 2011 to February 2014 were examined. Clinico-demographic characteristics, HT, functional outcomes and stroke recurrence were compared between patients with elevated hs-cTnT levels (≥14 ng/L) and patients with normal hs-cTnT levels (mortality and 3-month disability/mortality (all P≤0.029). After controlling for age, sex, hypertension, renal impairment and National Institutes of Health Stroke Scale score on admission, the risk of HT and 3-month mortality was, respectively, 4.0- and 5.5-fold higher in patients with elevated hs-cTnT levels than in patients with normal hs-cTnT levels. Elevated hs-cTnT levels are independently associated with HT and 3-month mortality in AIS patients with RHD. These results with a small cohort should be verified and extended in large studies.

Report from the banding lab

Science.gov (United States)

Tautin, J.

1995-01-01

Mr. Tautin reported on the seemingly everchanging structure of biological science units within the Interior Department. Current Congressional proposals would either change the name of the Bird Banding Lab's parent agency or make it part of the Geological Survey. The current Congress has not looked favorably on science budgets within the Interior Department, and the Banding Lab's budget is being squeezed ever tighter.

77 FR 17408 - Foreign-Trade Zone 204-Tri-Cities Area, TN/VA; Application for Reorganization Under Alternative...

Science.gov (United States)

2012-03-26

... Technology Park, 4509 West Stone Drive, Kingsport, Hawkins County, TN; Site 9 (134 acres, expires 6/30/15.... The grantee's proposed service area under the ASF would be the Counties of Sullivan, Hawkins, Greene...

Diversity, Equity, & Inclusion at Berkeley Lab

Science.gov (United States)

Berkeley Lab A-Z Index Directory Search Diversity, Equity, & Inclusion at Berkeley Lab Home Diversity & Inclusion Council Women Scientists & Engineers Council Employee Resource Groups -and culture of inclusion are key to attracting and engaging the brightest minds and furthering our

Magnetic Media Lab

Data.gov (United States)

Federal Laboratory Consortium — This lab specializes in tape certification and performance characterization of high density digital tape and isprepared to support the certification of standard size...

Crystallization Formulation Lab

Data.gov (United States)

Federal Laboratory Consortium — The Crystallization Formulation Lab fills a critical need in the process development and optimization of current and new explosives and energetic formulations. The...

Dengue virus inactivation by minipool TnBP/Triton X-45 treatment of plasma and cryoprecipitate.

Science.gov (United States)

Burnouf, T; Chou, M-L; Cheng, L-H; Li, Z-R; Wu, Y-W; El-Ekiaby, M; Tsai, K-H

2013-01-01

A minipool solvent/detergent (S/D; 1% TnBP/1% Triton X-45; 31°C) process was developed for viral inactivation of plasma and cryoprecipitate used for transfusion. The goal of this study was to determine the rate and extent of inactivation of dengue virus (DENV) during this process. DENV-1 was propagated using C6/36 mosquito cells to an infectivity titre close to 9 log and spiked (10% v/v) into individual plasma and cryoprecipitate samples from two distinct donors. Samples were taken right after spiking and during viral inactivation treatment by 1% TnBP-1% Triton X-45 at 31°C. DENV-1 infectivity was assessed on Vero E6 cells by a focus-forming assay (FFA). Culture medium and complement-inactivated plasma were used as experimental controls. Experiments were done in duplicate. DENV-1 infectivity was 7·5 log in spiked plasma and 7·1 and 7·3 log in spiked cryoprecipitate. There was no loss of DENV-1 infectivity in the spiked materials, nor in the controls not subjected to S/D treatment. No infectivity was found in plasma and cryoprecipitate subjected to S/D treatment at the first time-point evaluated (10 min). DENV-1 was strongly inactivated in plasma and cryoprecipitate, respectively, within 10 min of 1% TnBP/1% Triton X-45 treatment at 31°C. These data provide a reassurance of the safety of such S/D-treated plasma and cryoprecipitate with regard to the risk of transmission of all DENV serotypes and other flaviviruses. © 2012 The Author(s). Vox Sanguinis © 2012 International Society of Blood Transfusion.

On the almost everywhere convergence of the eigenfunction expansions from Liouville classes L_1^\\alpha ({T^N})

Science.gov (United States)

Ahmedov, Anvarjon; Materneh, Ehab; Zainuddin, Hishamuddin

2017-09-01

The relevance of waves in quantum mechanics naturally implies that the decomposition of arbitrary wave packets in terms of monochromatic waves plays an important role in applications of the theory. When eigenfunction expansions does not converge, then the expansions of the functions with certain smoothness should be considered. Such functions gained prominence primarily through their application in quantum mechanics. In this work we study the almost everywhere convergence of the eigenfunction expansions from Liouville classes L_p^α ({T^N}), related to the self-adjoint extension of the Laplace operator in torus TN . The sufficient conditions for summability is obtained using the modified Poisson formula. Isomorphism properties of the elliptic differential operators is applied in order to obtain estimation for the Fourier series of the functions from the classes of Liouville L_p^α .

Computer-based Astronomy Labs for Non-science Majors

Science.gov (United States)

Smith, A. B. E.; Murray, S. D.; Ward, R. A.

1998-12-01

We describe and demonstrate two laboratory exercises, Kepler's Third Law and Stellar Structure, which are being developed for use in an astronomy laboratory class aimed at non-science majors. The labs run with Microsoft's Excel 98 (Macintosh) or Excel 97 (Windows). They can be run in a classroom setting or in an independent learning environment. The intent of the labs is twofold; first and foremost, students learn the subject matter through a series of informational frames. Next, students enhance their understanding by applying their knowledge in lab procedures, while also gaining familiarity with the use and power of a widely-used software package and scientific tool. No mathematical knowledge beyond basic algebra is required to complete the labs or to understand the computations in the spreadsheets, although the students are exposed to the concepts of numerical integration. The labs are contained in Excel workbook files. In the files are multiple spreadsheets, which contain either a frame with information on how to run the lab, material on the subject, or one or more procedures. Excel's VBA macro language is used to automate the labs. The macros are accessed through button interfaces positioned on the spreadsheets. This is done intentionally so that students can focus on learning the subject matter and the basic spreadsheet features without having to learn advanced Excel features all at once. Students open the file and progress through the informational frames to the procedures. After each procedure, student comments and data are automatically recorded in a preformatted Lab Report spreadsheet. Once all procedures have been completed, the student is prompted for a filename in which to save their Lab Report. The lab reports can then be printed or emailed to the instructor. The files will have full worksheet and workbook protection, and will have a "redo" feature at the end of the lab for students who want to repeat a procedure.

The Udall Lab

Data.gov (United States)

Federal Laboratory Consortium — The Udall lab is interested in genome evolution and cotton genomics.The cotton genus ( Gossypium) is an extraordinarily diverse group with approximately 50 species...

Laser Research Lab

Data.gov (United States)

Federal Laboratory Consortium — The Laser Research lab is thecenter for the development of new laser sources, nonlinear optical materials, frequency conversion processes and laser-based sensors for...

LAB building a home for scientists

CERN Document Server

Fishman, Mark C

2017-01-01

Laboratories are both monasteries and space stations, redolent of the great ideas of generations past and of technologies to propel the future. Yet standard lab design has changed only little over recent years. Here Mark Fishman describes how to build labs as homes for scientists, to accommodate not just their fancy tools, but also their personalities. This richly illustrated book explores the roles of labs through history, from the alchemists of the Middle Ages to the chemists of the 19th and 20th centuries, and to the geneticists and structural biologists of today, and then turns to the special features of the laboratories Fishman helped to design in Cambridge, Shanghai, and Basel. Anyone who works in, or plans to build a lab, will enjoy this book, which will encourage them to think about how this special environment drives or impedes their important work.

Virtual labs in Leonardo da Vinci

Directory of Open Access Journals (Sweden)

Stanislaw Nagy

2006-10-01

Full Text Available This paper discusses the problem of virtual lab capabilities in the e-learning. Using combination of web conferencing and "virtual labs" capabilities, a new quality distance learning teaching is now in preparation and will be included in the course teaching to produce interactive, online simulations for the natural gas engineering studies. The activities are designed to enhance the existing curriculum and to include online assessments. A special care is devoted to the security problem between a server and a client computer. Several examples of the virtual labs related to the PVT thermodynamics, fluid flow, the natural gas well-testing, and thev gas network flow are prepared and tested. A major challenge for the 'CELGAS' system is in managing the delicate balance between the student collaboration and the isolation. Students may be encouraged to collaborate and work with each other, simulating their exploration of the lab material.

76 FR 53115 - Foreign-Trade Zone 77-Memphis, TN; Application for Temporary/Interim Manufacturing Authority...

Science.gov (United States)

2011-08-25

... Phone/Mobile Handset Kitting); Memphis, TN An application has been submitted to the Executive Secretary... authority to produce cell phones/mobile handsets kits (HTSUS 8517.12, HTSUS 8517.62, HTSUS 8517.69, duty... cell phone/mobile handset kits (duty free) for the foreign inputs noted above. In accordance with the...

Lab-on-fiber technology

CERN Document Server

Cusano, Andrea; Crescitelli, Alessio; Ricciardi, Armando

2014-01-01

This book focuses on a research field that is rapidly emerging as one of the most promising ones for the global optics and photonics community: the "lab-on-fiber" technology. Inspired by the well-established 'lab on-a-chip' concept, this new technology essentially envisages novel and highly functionalized devices completely integrated into a single optical fiber for both communication and sensing applications.Based on the R&D experience of some of the world's leading authorities in the fields of optics, photonics, nanotechnology, and material science, this book provides a broad and accurate de

Evaluation of oral microbiology lab curriculum reform.

Science.gov (United States)

Nie, Min; Gao, Zhen Y; Wu, Xin Y; Jiang, Chen X; Du, Jia H

2015-12-07

According to the updated concept of oral microbiology, the School of Stomatology, Wuhan University, has carried out oral microbiology teaching reforms during the last 5 years. There was no lab curriculum before 2009 except for a theory course of oral microbiology. The school has implemented an innovative curriculum with oral medicine characteristics to strengthen understanding of knowledge, cultivate students' scientific interest and develop their potential, to cultivate the comprehensive ability of students. This study was designed to evaluate the oral microbiology lab curriculum by analyzing student performance and perceptions regarding the curriculum from 2009 to 2013. The lab curriculum adopted modalities for cooperative learning. Students collected dental plaque from each other and isolated the cariogenic bacteria with selective medium plates. Then they purified the enrichment culture medium and identified the cariogenic strains by Gram stain and biochemical tests. Both quantitative and qualitative data for 5 years were analysed in this study. Part One of the current study assessed student performance in the lab from 2009 to 2013. Part Two used qualitative means to assess students' perceptions by an open questionnaire. The 271 study students' grades on oral microbiology improved during the lab curriculum: "A" grades rose from 60.5 to 81.2 %, and "C" grades fell from 28.4 to 6.3 %. All students considered the lab curriculum to be interesting and helpful. Quantitative and qualitative data converge to suggest that the lab curriculum has strengthened students' grasp of important microbiology-related theory, cultivated their scientific interest, and developed their potential and comprehensive abilities. Our student performance and perception data support the continued use of the innovative teaching system. As an extension and complement of the theory course, the oral microbiology lab curriculum appears to improve the quality of oral medicine education and help to

Innovation - A view from the Lab

Science.gov (United States)

The USDA Ag Lab in Peoria helps bridge the gap between agricultural producers and commercial manufacturers. In 2015, the Ag Lab, officially known as the Agricultural Research Service (ARS) National Center for Agricultural Utilization Research (NCAUR), is celebrating 75 years of research in Peoria. T...

The TN-RAM - a new cask for shipping high activity irradiated hardware

International Nuclear Information System (INIS)

Neider, T.; Hanson, A.S.

1993-01-01

Transnuclear, Inc. has developed a Type B(U) radioactive material transport packaging designed specifically for the transport of dry, irradiated, non-fuel bearing components (NFBC). The TN-RAM is a transport cask in the configuration of a right circular cylinder, fabricated from lead and stainless steel, with wood-filled impact limiters attached at both ends. The lead and stainless steel construction of the lid, walls, and bottom provides a shielding effectiveness of approximately 7.1 inches (18 cm) lead equivalent. (J.P.N.)

Environment monitoring using LabVIEW

International Nuclear Information System (INIS)

Hawtree, J.

1995-01-01

A system has been developed for electronically recording and monitoring temperature, humidity, and other environmental variables at the Silicon Detector Facility located in Lab D. The data is collected by LabVIEW software, which runs in the background on an Apple Macintosh. The software is completely portable between Macintosh, MS Windows, and Sun platforms. The hardware includes a Macintosh with 8 MB of RAM; an external ADC-1 analog-to-digital converter that uses a serial port; LabVIEW software; temperature sensors; humidity sensors; and other voltage/current sensing devices. ADC values are converted to ASCII strings and entered into files which are read over Ethernet. Advantages include automatic logging, automatic recovery after power interruptions, and the availability of stand-alone applications for other locations with inexpensive software and hardware

Concentrations, Distribution, and Ecological Risk Assessment of Heavy Metals in Daya Bay, China

Directory of Open Access Journals (Sweden)

Huijuan Tang

2018-06-01

Full Text Available Surface sediment samples were collected from 19 sites throughout Daya Bay, China to study the concentrations, spatial distributions, potential ecological risk, and possible sources of heavy, including metals copper (Cu, zinc (Zn, arsenic (As, cadmium (Cd, nickel (Ni, lead (Pb, mercury (Hg, and chromium (Cr. The mean concentrations of the eight heavy metals were 24, 109, 6.5, 0.09, 35.3, 26.8, 0.07, and 109 µg g−1, respectively. The concentrations of most heavy metals were within range of those recorded in previous years. The spatial distribution pattern of most heavy metals were similar, with lowest values recorded along the southeast coast and the open sea area; the highest values were recorded in the northern Daya Bay, especially the northwest. Cu, Zn, As, Cd, Pb, and Hg were classified as Class I, and Ni and Cr were classified as Class II according to the Sediment Quality Guidelines (SQGs of China. The potential ecological risk (Eif indices of Cu, Zn, As, Pb, Ni, and Cr specify that these metals pose low risk to the ecosystem of the Bay, whereas Cd and Hg pose a very high risk in some sites. The geoaccumulation indices (Igeo of Cu, Zn, As, Ni, and Cr specify weak or no pollution in Daya Bay, whereas those of Pb, Cd, and Hg in some sites indicate moderate or even high pollution. Spatial distribution, carbon/nitrogen analysis, Pearson correlation, and principal components analysis indicated that Cu, Zn, As, Pb, Ni, Cr, total organic carbon (TOC, and total nitrogen (TN originated from the same sources. Ballast water or sewage from the cargo ships that park at the harbors or anchor in the Bay were the important sources for Cu, Zn, As, Pb, Ni, Cr, TOC, and TN. Other anthropogenic sources, such as agricultural runoff and aquaculture, might also be responsible, whereas Hg and Cd originated from other point sources.

HTR fuel research in the HTR-TN network on the high flux reactor

Energy Technology Data Exchange (ETDEWEB)

Guidez, J.; Conrad, R.; Sevini, P.; Burghartz, M. [HFR Unit, Institute for Advanced Materials, European Commission, Joint Research Centre, Petten (Netherlands); Languille, A. [CEA Cadarache, 13 - Saint Paul lez Durance (France); Guillermier, P. [FRAMATOME ANP, 69 - Lyon (France); Bakker, K. [Nuclear Research and Consultancy Group, Petten (Netherlands); Nabielek, H. [Forschungszentrum Juelich (Germany)

2001-07-01

Foremost, this paper explains the economic and strategic reasons for the comeback of the HTR reactor as one of the most promising reactors in the future. To study all the points related to HTR technology, a European network called HTR-TN was created in April 2000, with actually twenty European companies involved. This paper explains the organisation of the network and the related task-groups. In the field of fuel, one of these task-groups works on the fuel cycle and another works on the fuel itself in order to validate by testing HTR fuel possibilities. To this aim, an experimental loop is under construction in the HFR reactor to test full-size pebble type fuel elements and another under study to test compact fuel possibilities. These loops are based on all the experience accumulated by the High Flux Reactor in the years 70-90, when a lot of test were performed for fuel and material for the HTR technology and the facility design uses all the existing HFR knowledge. In conclusion, a host of research work, co-ordinated in the frame of a European network HTR-TN has begun. and should allow in the near future a substantial progress in the knowledge of this very promising fuel. (author)

HTR fuel research in the HTR-TN network on the high flux reactor

International Nuclear Information System (INIS)

Guidez, J.; Conrad, R.; Sevini, P.; Burghartz, M.; Languille, A.; Guillermier, P.; Bakker, K.; Nabielek, H.

2001-01-01

Foremost, this paper explains the economic and strategic reasons for the comeback of the HTR reactor as one of the most promising reactors in the future. To study all the points related to HTR technology, a European network called HTR-TN was created in April 2000, with actually twenty European companies involved. This paper explains the organisation of the network and the related task-groups. In the field of fuel, one of these task-groups works on the fuel cycle and another works on the fuel itself in order to validate by testing HTR fuel possibilities. To this aim, an experimental loop is under construction in the HFR reactor to test full-size pebble type fuel elements and another under study to test compact fuel possibilities. These loops are based on all the experience accumulated by the High Flux Reactor in the years 70-90, when a lot of test were performed for fuel and material for the HTR technology and the facility design uses all the existing HFR knowledge. In conclusion, a host of research work, co-ordinated in the frame of a European network HTR-TN has begun. and should allow in the near future a substantial progress in the knowledge of this very promising fuel. (author)

A TALE of transposition: Tn3-like transposons play a major role in the spread of pathogenicity determinants of Xanthomonas citri and other xanthomonads.

Science.gov (United States)

Ferreira, Rafael Marini; de Oliveira, Amanda Carolina P; Moreira, Leandro M; Belasque, José; Gourbeyre, Edith; Siguier, Patricia; Ferro, Maria Inês T; Ferro, Jesus A; Chandler, Michael; Varani, Alessandro M

2015-02-17

Members of the genus Xanthomonas are among the most important phytopathogens. A key feature of Xanthomonas pathogenesis is the translocation of type III secretion system (T3SS) effector proteins (T3SEs) into the plant target cells via a T3SS. Several T3SEs and a murein lytic transglycosylase gene (mlt, required for citrus canker symptoms) are found associated with three transposition-related genes in Xanthomonas citri plasmid pXAC64. These are flanked by short inverted repeats (IRs). The region was identified as a transposon, TnXax1, with typical Tn3 family features, including a transposase and two recombination genes. Two 14-bp palindromic sequences within a 193-bp potential resolution site occur between the recombination genes. Additional derivatives carrying different T3SEs and other passenger genes occur in different Xanthomonas species. The T3SEs include transcription activator-like effectors (TALEs). Certain TALEs are flanked by the same IRs as found in TnXax1 to form mobile insertion cassettes (MICs), suggesting that they may be transmitted horizontally. A significant number of MICs carrying other passenger genes (including a number of TALE genes) were also identified, flanked by the same TnXax1 IRs and delimited by 5-bp target site duplications. We conclude that a large fraction of T3SEs, including individual TALEs and potential pathogenicity determinants, have spread by transposition and that TnXax1, which exhibits all of the essential characteristics of a functional transposon, may be involved in driving MIC transposition. We also propose that TALE genes may diversify by fork slippage during the replicative Tn3 family transposition. These mechanisms may play a crucial role in the emergence of Xanthomonas pathogenicity. Xanthomonas genomes carry many insertion sequences (IS) and transposons, which play an important role in their evolution and architecture. This study reveals a key relationship between transposons and pathogenicity determinants in

Experiential Learning of Digital Communication Using LabVIEW

Science.gov (United States)

Zhan, Wei; Porter, Jay R.; Morgan, Joseph A.

2014-01-01

This paper discusses the design and implementation of laboratories and course projects using LabVIEW in an instrumentation course. The pedagogical challenge is to enhance students' learning of digital communication using LabVIEW. LabVIEW was extensively used in the laboratory sessions, which better prepared students for the course projects. Two…

Effect of PCI on inflammatory factors, cTnI, MMP-9 and NT-pro BNP in patients with unstable angina pectoris

Directory of Open Access Journals (Sweden)

Ke-Tong Liu

2016-05-01

Full Text Available Objective: To investigate the effect of PCI on inflammatory factors, cTnI, MMP-9and NTpro BNP in patients with unstable angina pectoris. Methods: A total of 80 unstable angina pectoris patients were divided into observation group (40 cases and control group (40 cases. The observation group was given the therapy of PCI, and the control group was given coronary angiography. To observe the of inflammatory factors, cTnI, MMP-9 and NT-pro BNP were tested and compared before and after operation. Results: At 24 h after operation, CRP and IL-18 levels were increased significantly after treatment inoperation groups, there was no difference on inflammatory factors in control group, and had significant difference on inflammatory factors in two groups; At 24 h after operation, cTnI, MMP-9 and NT-pro BNP levels were increased significantly after treatment inoperation groups, there was no difference on inflammatory factors in control group, and had significant difference on inflammatory factors in two groups. Conclusion: PCI therapy can induce inflammation and myocardial injury in patients with unstable angina pectoris.

Baseball Physics: A New Mechanics Lab

Science.gov (United States)

Wagoner, Kasey; Flanagan, Daniel

2018-05-01

The game of baseball provides an interesting laboratory for experimenting with mechanical phenomena (there are many good examples in The Physics Teacher, available on Professor Alan Nathan's website, and discussed in Physics of Baseball & Softball). We have developed a lab, for an introductory-level physics course, that investigates many of these phenomena. The lab uses inexpensive, readily available equipment such as wooden baseball bats, baseballs, and actual Major League Baseball data. By the end of the lab, students have revisited many concepts they learned earlier in the semester and come away with an understanding of how to put seemingly disparate ideas together to analyze a fun sport.

Modeling Adsorption Based Filters (Bio-remediation of Heavy Metal Contaminated Water)

Science.gov (United States)

McCarthy, Chris

I will discuss kinetic models of adsorption, as well as models of filters based on those mechanisms. These mathematical models have been developed in support of our interdisciplinary lab group, which is centered at BMCC/CUNY (City University of New York). Our group conducts research into bio-remediation of heavy metal contaminated water via filtration. The filters are constructed out of biomass, such as spent tea leaves. The spent tea leaves are available in large quantities as a result of the industrial production of tea beverages. The heavy metals bond with the surfaces of the tea leaves (adsorption). The models involve differential equations, stochastic methods, and recursive functions. I will compare the models' predictions to data obtained from computer simulations and experimentally by our lab group. Funding: CUNY Collaborative Incentive Research Grant (Round 12); CUNY Research Scholars Program.

Living Lab voor Informatiemanagement in Agri-Food

NARCIS (Netherlands)

Wolfert, J.

2010-01-01

Het Living Lab is een specifieke open innovatie aanpak waarbij in feite het laboratorium naar de praktijk wordt gebracht. het Agri-Food Living lab is een informatiemanagementsysteem specifiek voor de agri-food sector.

Hydrogel Beads: The New Slime Lab?

Science.gov (United States)

Brockway, Debra; Libera, Matthew; Welner, Heidi

2011-01-01

Creating slime fascinates students. Unfortunately, though intrigue is at its peak, the educational aspect of this activity is often minimal. This article describes a chemistry lab that closely relates to the slime lab and allows high school students to explore the concepts of chemical bonding, properties, and replacement reactions. It involves the…

76 FR 60802 - Foreign-Trade Zone 77-Memphis, TN; Withdrawal of Request for Temporary/Interim Manufacturing...

Science.gov (United States)

2011-09-30

..., Inc., (Cell Phone/Mobile Handset Kitting). Memphis, TN Notice is hereby given of the withdrawal of the application of the City of Memphis, grantee of FTZ 77, requesting temporary/interim manufacturing authority on behalf of Flextronics Logistics USA, Inc. in Memphis Tennessee. The application was filed on August 19...

GeoLab: A Geological Workstation for Future Missions

Science.gov (United States)

Evans, Cynthia; Calaway, Michael; Bell, Mary Sue; Li, Zheng; Tong, Shuo; Zhong, Ye; Dahiwala, Ravi

2014-01-01

The GeoLab glovebox was, until November 2012, fully integrated into NASA's Deep Space Habitat (DSH) Analog Testbed. The conceptual design for GeoLab came from several sources, including current research instruments (Microgravity Science Glovebox) used on the International Space Station, existing Astromaterials Curation Laboratory hardware and clean room procedures, and mission scenarios developed for earlier programs. GeoLab allowed NASA scientists to test science operations related to contained sample examination during simulated exploration missions. The team demonstrated science operations that enhance theThe GeoLab glovebox was, until November 2012, fully integrated into NASA's Deep Space Habitat (DSH) Analog Testbed. The conceptual design for GeoLab came from several sources, including current research instruments (Microgravity Science Glovebox) used on the International Space Station, existing Astromaterials Curation Laboratory hardware and clean room procedures, and mission scenarios developed for earlier programs. GeoLab allowed NASA scientists to test science operations related to contained sample examination during simulated exploration missions. The team demonstrated science operations that enhance the early scientific returns from future missions and ensure that the best samples are selected for Earth return. The facility was also designed to foster the development of instrument technology. Since 2009, when GeoLab design and construction began, the GeoLab team [a group of scientists from the Astromaterials Acquisition and Curation Office within the Astromaterials Research and Exploration Science (ARES) Directorate at JSC] has progressively developed and reconfigured the GeoLab hardware and software interfaces and developed test objectives, which were to 1) determine requirements and strategies for sample handling and prioritization for geological operations on other planetary surfaces, 2) assess the scientific contribution of selective in-situ sample

Heidegeer a Kierkegaard: pojetí boha a posvátného

Directory of Open Access Journals (Sweden)

Jiří Olšovský

2010-05-01

Full Text Available Text je zaměřen zejména na posvátný charakter světa a přírody, jak se zračí v pojetí bytí a božského u Heideggera a Kierkegaarda. Bytí zde zůstává tématem, spolu se základní péčí a starostí o svět a věci v něm. Ukazuje se, že spodní proud Heideggerova myšlení je znovu-probuzení „zbožného myšlení“ a rezervovanost. Zejména článek chce upozornit na Heideggerovy odkazy k mysticismu a Kierkegaardův „pramen víry“. V úvahách nad Heideggerovým dílem je třeba se zamyslet, zda otázka Boha může být rozhodnuta jen v blízkosti bytí a svatosti. O noumenální charakter naší „zahrady“ je nezbytné znovu náležitě pečovat, a zaposlouchat se proto do řeči těch, kteří byli s to se přiblížit k bytí a božskému, k posvátnému pobývání na zemi.

CERN Technical Training 2006: LabVIEW Course Sessions (September-December 2006)

CERN Multimedia

2006-01-01

The following LabVIEW course sessions are currently scheduled in the framework of the CERN Technical Training Programme 2006, and in collaboration with National Instruments (CH): LabVIEW Basics 1 (course in English): 11-13.9.2006 (3 days, only 3 places available) LabVIEW Basics 2 (course in English): 14-15.9.2006 (2 days) LabVIEW: Working efficiently with LabVIEW 8 (course in English): 18.9.2006 (1 day) **NEW COURSE** LabVIEW Application Development (course in English): 13-15.11.2006 (3 days. Pre-requisite: LabVIEW Basics I ans II, or equivalent experience) LabVIEW Advanced Programming (course in English): 16-17.11.2006 (2 days. Pre-requisite: LabVIEW Application Development, or equivalent experience) LabVIEW Base 1 (course in French): 4-6.12.2006 (3 days, only 1 place available) LabVIEW Base 2 (course in French): 7-8.12.2006 (2 days) If you are interested in attending any of the above course sessions, please discuss with your supervisor and/or your DTO, and apply electronically via EDH from the cour...

Anal Carcinoma: Impact of TN Category of Disease on Survival, Disease Relapse, and Colostomy Failure in US Gastrointestinal Intergroup RTOG 98-11 Phase 3 Trial

Science.gov (United States)

Gunderson, Leonard L.; Moughan, Jennifer; Ajani, Jaffer A.; Pedersen, John E.; Winter, Kathryn A.; Benson, Al B.; Thomas, Charles R.; Mayer, Robert J.; Haddock, Michael G.; Rich, Tyvin A.; Willett, Christopher G.

2013-01-01

Purpose The long-term update of US GI Intergroup RTOG 98-11 anal cancer trial found that concurrent chemoradiation (CCRT) with fluorouracil (5-FU) plus mitomycin had a significant impact on disease-free survival (DFS) and overall survival (OS) compared with induction plus concurrent 5-FU plus cisplatin. The intent of the current analysis was to determine the impact of tumor node (TN) category of disease on survival (DFS and OS), colostomy failure (CF), and relapse (local-regional failure [LRF] and distant metastases [DM]) in this patient group. Methods and Materials DFS and OS were estimated univariately by using the Kaplan-Meier method, and 6 TN categories were compared by the log–rank test (T2N0, T3N0, T4N0, T2N1-3, T3N1-3, and T4N1-3). Time to relapse and colostomy were estimated by the cumulative incidence method, and TN categories were compared using Gray’s test. Results Of 682 patients, 620 were analyzable for outcomes by TN category. All endpoints showed statistically significant differences among the TN categories of disease (OS, P<.0001; DFS, P<.0001; LRF, P<.0001; DM, P=.0011; CF, P=.01). Patients with the poorest OS, DFS, and LRF outcomes were those with T3-4N-positive (+) disease. CF was lowest for T2N0 and T2N+ (11%, 11%, respectively) and worst for the T4N0, T3N+, and T4N+ categories (26%, 27%, 24%, respectively). Conclusions TN category of disease has a statistically significant impact on OS, DFS, LRF, DM, and CF in patients treated with CCRT and provides excellent prognostic information for outcomes in patients with anal carcinoma. Significant challenges remain for patients with T4N0 and T3-4N+ categories of disease with regard to survival, relapse, and CF and lesser challenges for T2-3N0/T2N+ categories. PMID:24035327

Anal Carcinoma: Impact of TN Category of Disease on Survival, Disease Relapse, and Colostomy Failure in US Gastrointestinal Intergroup RTOG 98-11 Phase 3 Trial

Energy Technology Data Exchange (ETDEWEB)

Gunderson, Leonard L., E-mail: gunderson.leonard@mayo.edu [Mayo Clinic Cancer Center, Scottsdale, Arizona (United States); Moughan, Jennifer [Radiation Therapy Oncology Group, Philadelphia, Pennsylvania (United States); Ajani, Jaffer A. [The University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Pedersen, John E. [Cross Cancer Institute, Edmonton, Alberta (Canada); Winter, Kathryn A. [Radiation Therapy Oncology Group, Philadelphia, Pennsylvania (United States); Benson, Al B. [Northwestern University, Chicago, Illinois (United States); Thomas, Charles R. [Knight Cancer Institute/Oregon Health and Science University, Portland, Oregon (United States); Mayer, Robert J. [Dana-Farber Cancer Institute, Boston, Massachusetts (United States); Haddock, Michael G. [Mayo Clinic Cancer Center, Rochester, Minnesota (United States); Rich, Tyvin A. [University of Virginia, Charlottesville, Virginia (United States); Willett, Christopher G. [Duke University, Durham, North Carolina (United States)

2013-11-15

Purpose: The long-term update of US GI Intergroup RTOG 98-11 anal cancer trial found that concurrent chemoradiation (CCRT) with fluorouracil (5-FU) plus mitomycin had a significant impact on disease-free survival (DFS) and overall survival (OS) compared with induction plus concurrent 5-FU plus cisplatin. The intent of the current analysis was to determine the impact of tumor node (TN) category of disease on survival (DFS and OS), colostomy failure (CF), and relapse (local-regional failure [LRF] and distant metastases [DM]) in this patient group. Methods and Materials: DFS and OS were estimated univariately by using the Kaplan-Meier method, and 6 TN categories were compared by the log–rank test (T2N0, T3N0, T4N0, T2N1-3, T3N1-3, and T4N1-3). Time to relapse and colostomy were estimated by the cumulative incidence method, and TN categories were compared using Gray's test. Results: Of 682 patients, 620 were analyzable for outcomes by TN category. All endpoints showed statistically significant differences among the TN categories of disease (OS, P<.0001; DFS, P<.0001; LRF, P<.0001; DM, P=.0011; CF, P=.01). Patients with the poorest OS, DFS, and LRF outcomes were those with T3-4N-positive (+) disease. CF was lowest for T2N0 and T2N+ (11%, 11%, respectively) and worst for the T4N0, T3N+, and T4N+ categories (26%, 27%, 24%, respectively). Conclusions: TN category of disease has a statistically significant impact on OS, DFS, LRF, DM, and CF in patients treated with CCRT and provides excellent prognostic information for outcomes in patients with anal carcinoma. Significant challenges remain for patients with T4N0 and T3-4N+ categories of disease with regard to survival, relapse, and CF and lesser challenges for T2-3N0/T2N+ categories.

Anal Carcinoma: Impact of TN Category of Disease on Survival, Disease Relapse, and Colostomy Failure in US Gastrointestinal Intergroup RTOG 98-11 Phase 3 Trial

International Nuclear Information System (INIS)

Gunderson, Leonard L.; Moughan, Jennifer; Ajani, Jaffer A.; Pedersen, John E.; Winter, Kathryn A.; Benson, Al B.; Thomas, Charles R.; Mayer, Robert J.; Haddock, Michael G.; Rich, Tyvin A.; Willett, Christopher G.

2013-01-01

Purpose: The long-term update of US GI Intergroup RTOG 98-11 anal cancer trial found that concurrent chemoradiation (CCRT) with fluorouracil (5-FU) plus mitomycin had a significant impact on disease-free survival (DFS) and overall survival (OS) compared with induction plus concurrent 5-FU plus cisplatin. The intent of the current analysis was to determine the impact of tumor node (TN) category of disease on survival (DFS and OS), colostomy failure (CF), and relapse (local-regional failure [LRF] and distant metastases [DM]) in this patient group. Methods and Materials: DFS and OS were estimated univariately by using the Kaplan-Meier method, and 6 TN categories were compared by the log–rank test (T2N0, T3N0, T4N0, T2N1-3, T3N1-3, and T4N1-3). Time to relapse and colostomy were estimated by the cumulative incidence method, and TN categories were compared using Gray's test. Results: Of 682 patients, 620 were analyzable for outcomes by TN category. All endpoints showed statistically significant differences among the TN categories of disease (OS, P<.0001; DFS, P<.0001; LRF, P<.0001; DM, P=.0011; CF, P=.01). Patients with the poorest OS, DFS, and LRF outcomes were those with T3-4N-positive (+) disease. CF was lowest for T2N0 and T2N+ (11%, 11%, respectively) and worst for the T4N0, T3N+, and T4N+ categories (26%, 27%, 24%, respectively). Conclusions: TN category of disease has a statistically significant impact on OS, DFS, LRF, DM, and CF in patients treated with CCRT and provides excellent prognostic information for outcomes in patients with anal carcinoma. Significant challenges remain for patients with T4N0 and T3-4N+ categories of disease with regard to survival, relapse, and CF and lesser challenges for T2-3N0/T2N+ categories

Virtual Lab for Wireless Sensor Networks

Directory of Open Access Journals (Sweden)

PICOVICI, D.

2008-06-01

Full Text Available This article details an experimental system developed to enhance the education and research in the area of wireless networks technologies. The system referred, as Virtual Lab (VL is primarily targeting first time users or users with limited experience in programming and using wireless sensor networks. The VL enables a set of predefined sensor networks to be remotely accessible and controlled for constructive and time-efficient experimentation. In order to facilitate the user's wireless sensor applications, the VL is using three main components: a a Virtual Lab Motes (VLM, representing the wireless sensor, b a Virtual Lab Client (VLC, representing the user's tool to interact with the VLM and c a Virtual Lab Server (VLS representing the software link between the VLM and VLC. The concept has been proven using the moteiv produced Tmote Sky modules. Initial experimental use clearly demonstrates that the VL approach reduces dramatically the learning curve involved in programming and using the associated wireless sensor nodes. In addition the VL allows the user's focus to be directed towards the experiment and not towards the software programming challenges.

Electrodialytic removal of heavy metals from different fly ashes. Influence of heavy metal speciation in the ashes

DEFF Research Database (Denmark)

Pedersen, Anne Juul; Ottosen, Lisbeth M.; Villumsen, Arne

2003-01-01

Electrodialytic Remediation has recently been suggested as a potential method for removal of heavy metals from fly ashes. In this work electrodialytic remediation of three different fly ashes, i.e. two municipal solid waste incinerator (MSWI) fly ashes and one wood combustion fly ash was studied...... in lab scale, and the results were discussed in relation to the expected heavy metal speciation in the ashes. In initial leaching experiments the pH-dependent desorption characteristics of the heavy metals Cd, Pb, Zn and Cu were analogous in the two MSWI ashes, and thus it was expected......-moval efficiencies were observed, especially for Pb and Zn. Cd, the sole heavy metal of environmental concern in the wood ash, was found more tightly bonded in this ash than in the two MSWI ashes. It was suggested that complex Cd-silicates are likely phases in the wood ash whereas more soluble, condensed phases...

Magnetic Viscous Drag for Friction Labs

Science.gov (United States)

Gaffney, Chris; Catching, Adam

2016-01-01

The typical friction lab performed in introductory mechanics courses is usually not the favorite of either the student or the instructor. The measurements are not all that easy to make, and reproducibility is usually a troublesome issue. This paper describes the augmentation of such a friction lab with a study of the viscous drag on a magnet…

Automatic creation of LabVIEW network shared variables

International Nuclear Information System (INIS)

Kluge, T.; Schroeder, H.

2012-01-01

We are in the process of preparing the LabVIEW controlled system components of our Solid State Direct Drive experiments for the integration into a Supervisory Control And Data Acquisition (SCADA) or distributed control system. The predetermined route to this is the generation of LabVIEW network shared variables that can easily be exported by LabVIEW to the SCADA system using OLE for Process Control (OPC) or other means. Many repetitive tasks are associated with the creation of the shared variables and the required code. We are introducing an efficient and inexpensive procedure that automatically creates shared variable libraries and sets default values for the shared variables. Furthermore, LabVIEW controls are created that are used for managing the connection to the shared variable inside the LabVIEW code operating on the shared variables. The procedure takes as input an XML spread-sheet defining the required input. The procedure utilizes XSLT and LabVIEW scripting. In a later state of the project the code generation can be expanded to also create code and configuration files that will become necessary in order to access the shared variables from the SCADA system of choice. (authors)

Microstructural characterization of LaB6-ZrB2 eutectic composites

International Nuclear Information System (INIS)

Wang Shengchang; Wei, W.J.; Zhang Litong

2003-01-01

Detail microstructure of LaB 6 -ZrB 2 composites has been characterized by TEM and HRTEM. The directionally solidified ZrB 2 fibers in LaB 6 matrix near LaB 6 -ZrB 2 eutectics present at least three growing relationship systems. In addition to previous report of [001]LaB 6 / [0001]ZrB 2 relationship, [0 anti 11]LaB 6 / [0001]ZrB 2 and [1 anti 20]LaB 6 / [0001]ZrB 2 . were identified. Different with [001]LaB 6 / [0001]ZrB 2 system, the interfaces of [0 anti 11]LaB 6 / [0001]ZrB 2 and [1 anti 20]LaB 6 / [0001]ZrB 2 . show non-coherent and clean interfaces. There is neither glassy phase nor reaction products found at the interfaces (orig.)

Ecological risk assessment of heavy metals in sediment and human health risk assessment of heavy metals in fishes in the middle and lower reaches of the Yangtze River basin

Energy Technology Data Exchange (ETDEWEB)

Yi Yujun [State Key Laboratory of Water Environment Simulation and Pollution Control, School of Environment, Beijing Normal University, Beijing 100875 (China); Yang Zhifeng, E-mail: zfyang@bnu.edu.cn [State Key Laboratory of Water Environment Simulation and Pollution Control, School of Environment, Beijing Normal University, Beijing 100875 (China); Zhang Shanghong [Renewable Energy School, North China Electric Power University, Beijing 102206 (China)

2011-10-15

The concentrations of heavy metals (Cr, Cd, Hg, Cu, Zn, Pb and As) in the water, sediment, and fish were investigated in the middle and lower reaches of the Yangtze River, China. Potential ecological risk analysis of sediment heavy metal concentrations indicated that six sites in the middle reach, half of the sites in the lower reach, and two sites in lakes, posed moderate or considerable ecological risk. Health risk analysis of individual heavy metals in fish tissue indicated safe levels for the general population and for fisherman but, in combination, there was a possible risk in terms of total target hazard quotients. Correlation analysis and PCA found that heavy metals (Hg, Cd, Pb, Cr, Cu, and Zn) may be mainly derived from metal processing, electroplating industries, industrial wastewater, and domestic sewage. Hg may also originate from coal combustion. Significant positive correlations between TN and As were observed. - Highlights: > Field survey, test and relationship of the concentrations of heavy metals in the water, sediment, and fish. > Potential ecological risk assessment of heavy metals in sediment. > Non-cancer health risk assessment of heavy metals in fish tissue. > Possible pollution source of heavy metals analyzed. - Possible ecological risk of sediment and slight non-cancer health risk of eating fish were found in the middle and lower reaches of the Yangtze River.

Ecological risk assessment of heavy metals in sediment and human health risk assessment of heavy metals in fishes in the middle and lower reaches of the Yangtze River basin

International Nuclear Information System (INIS)

Yi Yujun; Yang Zhifeng; Zhang Shanghong

2011-01-01

The concentrations of heavy metals (Cr, Cd, Hg, Cu, Zn, Pb and As) in the water, sediment, and fish were investigated in the middle and lower reaches of the Yangtze River, China. Potential ecological risk analysis of sediment heavy metal concentrations indicated that six sites in the middle reach, half of the sites in the lower reach, and two sites in lakes, posed moderate or considerable ecological risk. Health risk analysis of individual heavy metals in fish tissue indicated safe levels for the general population and for fisherman but, in combination, there was a possible risk in terms of total target hazard quotients. Correlation analysis and PCA found that heavy metals (Hg, Cd, Pb, Cr, Cu, and Zn) may be mainly derived from metal processing, electroplating industries, industrial wastewater, and domestic sewage. Hg may also originate from coal combustion. Significant positive correlations between TN and As were observed. - Highlights: → Field survey, test and relationship of the concentrations of heavy metals in the water, sediment, and fish. → Potential ecological risk assessment of heavy metals in sediment. → Non-cancer health risk assessment of heavy metals in fish tissue. → Possible pollution source of heavy metals analyzed. - Possible ecological risk of sediment and slight non-cancer health risk of eating fish were found in the middle and lower reaches of the Yangtze River.

Flexible HVAC System for Lab or Classroom.

Science.gov (United States)

Friedan, Jonathan

2001-01-01

Discusses an effort to design a heating, ventilation, and air conditioning system flexible enough to accommodate an easy conversion of classrooms to laboratories and dry labs to wet labs. The design's energy efficiency and operations and maintenance are examined. (GR)

The Dynamics and Facilitation of a Living Lab Construct

DEFF Research Database (Denmark)

Brønnum, Louise; Nielsen, Louise Møller

2013-01-01

During the last decade Living Labs have established itself as an attractive innovation approach. Living Labs are an interesting construction because it offers a collaboration platform for dynamic interaction with users in all the project phases. Living Labs frame knowledge about actors in their o...

Assessing Usage and Maximizing Finance Lab Impact: A Case Exploration

Science.gov (United States)

Noguera, Magdy; Budden, Michael Craig; Silva, Alberto

2011-01-01

This paper reports the results of a survey conducted to assess students' usage and perceptions of a finance lab. Finance labs differ from simple computer labs as they typically contain data boards, streaming market quotes, terminals and software that allow for real-time financial analyses. Despite the fact that such labs represent significant and…

Troponin C Mutations Partially Stabilize the Active State of Regulated Actin and Fully Stabilize the Active State When Paired with Δ14 TnT.

Science.gov (United States)

Baxley, Tamatha; Johnson, Dylan; Pinto, Jose R; Chalovich, Joseph M

2017-06-13

Striated muscle contraction is regulated by the actin-associated proteins tropomyosin and troponin. The extent of activation of myosin ATPase activity is lowest in the absence of both Ca 2+ and activating cross-bridges (i.e., S1-ADP or rigor S1). Binding of activating species of myosin to actin at a saturating Ca 2+ concentration stabilizes the most active state (M state) of the actin-tropomyosin-troponin complex (regulated actin). Ca 2+ binding alone produces partial stabilization of the active state. The extent of stabilization at a saturating Ca 2+ concentration depends on the isoform of the troponin subunits, the phosphorylation state of troponin, and, in the case of cardiac muscle, the presence of hypertrophic cardiomyopathy-producing mutants of troponin T and troponin I. Cardiac dysfunction is also associated with mutations of troponin C (TnC). Troponin C mutants A8V, C84Y, and D145E increase the Ca 2+ sensitivity of ATPase activity. We show that these mutants change the distribution of regulated actin states. The A8V and C84Y TnC mutants decreased the inactive B state distribution slightly at low Ca 2+ concentrations, but the D145E mutants had no effect on that state. All TnC mutants increased the level of the active M state compared to that of the wild type, at a saturating Ca 2+ concentration. Troponin complexes that contained two mutations that stabilize the active M state, A8V TnC and Δ14 TnT, appeared to be completely in the active state in the presence of only Ca 2+ . Because Ca 2+ gives full activation, in this situation, troponin must be capable of positioning tropomyosin in the active M state without the need for rigor myosin binding.

Innovative Educational Practice: Using Virtual Labs in the Secondary Classroom

Directory of Open Access Journals (Sweden)

Marcel Satsky Kerr, PhD

2004-07-01

Full Text Available Two studies investigated the effectiveness of teaching science labs online to secondary students. Study 1 compared achievement among students instructed using hands-on Chemistry labs versus those instructed using virtual Chemistry labs (eLabs. Study 2 compared the same groups of students again while both teachers instructed using hands-on Chemistry labs to determine whether teacher or student characteristics may have affected Study 1’s findings. Participants were high school Chemistry students from a Central Texas Independent School District. Results indicated that: students learn science effectively online, schools may experience cost savings from delivering labs online, and students gain valuable technology skills needed later in college and in the workplace.

Designing virtual science labs for the Islamic Academy of Delaware

Science.gov (United States)

AlZahrani, Nada Saeed

Science education is a basic part of the curriculum in modern day classrooms. Instructional approaches to science education can take many forms but hands-on application of theory via science laboratory activities for the learner is common. Not all schools have the resources to provide the laboratory environment necessary for hands-on application of science theory. Some settings rely on technology to provide a virtual laboratory experience instead. The Islamic Academy of Delaware (IAD), a typical community-based organization, was formed to support and meet the essential needs of the Muslim community of Delaware. IAD provides science education as part of the overall curriculum, but cannot provide laboratory activities as part of the science program. Virtual science labs may be a successful model for students at IAD. This study was conducted to investigate the potential of implementing virtual science labs at IAD and to develop an implementation plan for integrating the virtual labs. The literature has shown us that the lab experience is a valuable part of the science curriculum (NBPTS, 2013, Wolf, 2010, National Research Council, 1997 & 2012). The National Research Council (2012) stressed the inclusion of laboratory investigations in the science curriculum. The literature also supports the use of virtual labs as an effective substitute for classroom labs (Babateen, 2011; National Science Teachers Association, 2008). Pyatt and Simms (2011) found evidence that virtual labs were as good, if not better than physical lab experiences in some respects. Although not identical in experience to a live lab, the virtual lab has been shown to provide the student with an effective laboratory experience in situations where the live lab is not possible. The results of the IAD teacher interviews indicate that the teachers are well-prepared for, and supportive of, the implementation of virtual labs to improve the science education curriculum. The investigator believes that with the

CREATINE PHOSPHOKINASE ISOENZYME-MB MASS (CK-MB MASS AND TROPONIN I (cTnI IN DOGS (Canis familiaris

Directory of Open Access Journals (Sweden)

Millena Vidal Freitas

2015-07-01

Full Text Available Cardiac markers, especially CK-MB mass and cTnI, have an essential role in both human and veterinary clinical and surgical cardiology, allowing a more precise and accurate diagnosis in myocardial lesions. The goal of this work was to measure these cardiac markers in veterinary medicine, improve their use and provide information about these laboratory methods that allow non-invasive health monitoring of the myocardial cell. Parameters quantification was obtained from sera of healthy dogs examined during routine procedures at the Small Animal Veterinary Hospital of Universidade Estadual do Norte Fluminense Darcy Ribeiro. The human chemiluminescence essay turbo kit (IMMULITE Turbo, Siemens ® test proved to be effective in canine species for both CK-MB mass and cTnI. In addition, the values found for CK-MB will significantly contribute to clinical routine or experimental work with dogs.

The VanE operon in Enterococcus faecalis N00-410 is found on a putative integrative and conjugative element, Tn6202.

Science.gov (United States)

Boyd, D A; Mulvey, M R

2013-02-01

To date no complete genetic structure of acquired DNA harbouring a d-Ala-d-Ser operon in an Enterococcus is known. We wished to characterize the acquired DNA harbouring the vanE operon located in the Enterococcus faecalis N00-410 chromosome. Whole genome sequencing of E. faecalis N00-410 was conducted by massively parallel sequencing. Two sequence contigs harbouring the vanE region were linked by PCR and the acquired DNA harbouring the vanE operon was completely characterized. Excision/integration of the region was determined by PCR and transfer attempted by conjugation. The regions flanking the vanE operon were analysed and a total of 42 open reading frames were identified in a region flanked by inverted terminal and direct repeats (Tn6202). Tn6202 could be excised from the chromosome, circularized and the target site rejoined, but transfer could not be demonstrated. The vanE operon was found on the putative integrative and conjugative element Tn6202 in the E. faecalis N00-410 chromosome. This represents the first characterization of acquired DNA harbouring a D-Ala-D-Ser operon.

Evaluation of Factors Influencing Lab-Scale Studies to Determine Heavy Metal Removal by Six Sorbents for Stormwater Treatment

Directory of Open Access Journals (Sweden)

Maximilian Huber

2016-02-01

Full Text Available For the development of decentralized treatment systems for road runoff, the determination of pollutant removal capacities is essential. The aim of this study was to evaluate the impact of boundary conditions on the simultaneous removal of copper, nickel, and zinc by six sorbents used for urban stormwater treatment (i.e., granular activated alumina, anthracite, granular reactivated carbon, granular ferric hydroxide, calcium carbonate, and granular activated lignite. For batch experiments, capacities were determined at initial concentrations within the range of 2.5–180 mg/L with a rotary shaker. Further influences were investigated: the use of a horizontal shaker for concentrations of up to 1080 mg/L, a variation of the initial pH value (5 and 7, and the presence of a buffer. Furthermore, the influences of the filtration process on the capacities were studied. Kinetic experiments were conducted for contact times between 5 min and 120 min. Lab-scale column experiments with inflow concentrations of 2.5 mg/L (copper and nickel and 5.0 mg/L (zinc at an initial pH of 5 and a contact time of 11 min were performed for comparison. Selected experiments were subsequently carried out with changes in initial concentrations and contact time. One result is that it is essential to conduct batch experiments with the metals of interest. The capacities determined by column experiments deviated from batch experiments. Batch experiments under well-defined conditions can be used to evaluate different production batches. Column experiments give a more faithful capacity by considering realistic boundary conditions and should be preferred to determine efficiencies and service lives.

Fracture resistance of prepared premolars restored with bonded new lab composite and all-ceramic inlay/onlay restorations: Laboratory study.

Science.gov (United States)

Wafaie, Ramy Ahmed; Ibrahim Ali, Ashraf; Mahmoud, Salah Hasab

2018-01-25

To assess the influence of new light curing lab composite, lithium-disilicate glass-ceramic and yttrium-stabilized zirconia-based ceramic on the fracture resistance of maxillary premolars with class II inlay and onlay preparations. Seventy sound maxillary premolars were divided randomly into seven main groups. The first group was left intact (control group). The remaining six groups were prepared with inlay and onlay cavities and restored with lab composite (SR Nexco), lithium-disilicate glass-ceramic (IPS e.max Press) and yttrium-stabilized zirconia-based ceramic (ICE Zirkon). The restorations were cemented with luting resin composite (Variolink N). All specimens were thermocycled 5000 cycles between 5°C ± 2°C and 55°C ± 2°C and were then cyclic loaded for 500 000 cycles. The specimens were subjected to a compressive load in a universal testing machine using a metal sphere until fracture occurred. The results were analyzed by 2-way ANOVA and Tukey HSD post hoc tests. The level of significance was set at P  .05). However, statistically significant differences were found among the means of control group and the groups restored with lab composite inlays, lab composite onlays, pressable glass ceramic inlays and pressable glass ceramic onlays (P lab composite is used. Conversely, when a ceramic material being used, the prepared teeth for inlay and onlay restorations showed a comparable strength to the intact teeth especially zirconia ceramic. Premolar teeth restored with zirconia ceramic inlays and onlays exhibited fracture resistance comparable to intact teeth. © 2018 Wiley Periodicals, Inc.

Using lab notebooks to examine students' engagement in modeling in an upper-division electronics lab course

Science.gov (United States)

Stanley, Jacob T.; Su, Weifeng; Lewandowski, H. J.

2017-12-01

We demonstrate how students' use of modeling can be examined and assessed using student notebooks collected from an upper-division electronics lab course. The use of models is a ubiquitous practice in undergraduate physics education, but the process of constructing, testing, and refining these models is much less common. We focus our attention on a lab course that has been transformed to engage students in this modeling process during lab activities. The design of the lab activities was guided by a framework that captures the different components of model-based reasoning, called the Modeling Framework for Experimental Physics. We demonstrate how this framework can be used to assess students' written work and to identify how students' model-based reasoning differed from activity to activity. Broadly speaking, we were able to identify the different steps of students' model-based reasoning and assess the completeness of their reasoning. Varying degrees of scaffolding present across the activities had an impact on how thoroughly students would engage in the full modeling process, with more scaffolded activities resulting in more thorough engagement with the process. Finally, we identified that the step in the process with which students had the most difficulty was the comparison between their interpreted data and their model prediction. Students did not use sufficiently sophisticated criteria in evaluating such comparisons, which had the effect of halting the modeling process. This may indicate that in order to engage students further in using model-based reasoning during lab activities, the instructor needs to provide further scaffolding for how students make these types of experimental comparisons. This is an important design consideration for other such courses attempting to incorporate modeling as a learning goal.

Cassandra - WP400 - final report of living lab 2

NARCIS (Netherlands)

Engler, M.; Klievink, A.J.

2014-01-01

This CASSANDRA LL2 final deliverable contains all information regarding the CASSANDRA Living Lab Europe – USA via Bremerhaven including information from two intermediate reports (CASSANDRA D4.21 and D4.22) about the very same Living Lab handed in during runtime of the Living Lab. CASSANDRA Living

Comunicación USB entre aplicaciones desarrolladas en LabVIEW y microcontroladores de Silicon Labs

Directory of Open Access Journals (Sweden)

Julio César Herrera Benítez

2013-06-01

Full Text Available El presente artículo trata sobre la utilización de LabVIEW para establecer comunicación USB con microcontroladores de la familia 8051 de Silicon Laboratories, utilizando un driver desarrollado por dicha compañía. En el documento se incluye una descripción de este driver, así como de las funciones principales que permiten el manejo del mismo, las cuales se encuentran en una biblioteca de enlace dinámico. El artículo contiene además una metodología básica para el uso de estas funciones y una explicación detallada a través de un ejemplo, donde se ilustra como cargar y configurar las mismas con el ambiente de desarrollo LabVIEW. Finalmente se muestran dos ejemplos de la utilización del driver a partir de una biblioteca de funciones USB creada en LabVIEW para la comunicación con un microcontrolador.

USNA DIGITAL FORENSICS LAB

Data.gov (United States)

Federal Laboratory Consortium — To enable Digital Forensics and Computer Security research and educational opportunities across majors and departments. Lab MissionEstablish and maintain a Digital...

Fabrication and Prototyping Lab

Data.gov (United States)

Federal Laboratory Consortium — Purpose: The Fabrication and Prototyping Lab for composite structures provides a wide variety of fabrication capabilities critical to enabling hands-on research and...

Digital Design with KP-Lab

Directory of Open Access Journals (Sweden)

D. Ponta

2007-08-01

Full Text Available KP-Lab is an EU Integrated Project envisioning a learning system that facilitates innovative practices of sharing, creating and working with knowledge in education and workplaces. The project exploits a novel pedagogical view, the knowledge-creation metaphor of learning. According to such “trialogical” approach, cognition arises through collaborative work in systematically developing shared “knowledge artefacts”, such as concepts, plans, material products, or social practices. The paper presents the plan of a pilot course to test the KP-Lab methodologies and tools in the field of Digital Design.

Modifying Cookbook Labs.

Science.gov (United States)

Clark, Robert, L.; Clough, Michael P.; Berg, Craig A.

2000-01-01

Modifies an extended lab activity from a cookbook approach for determining the percent mass of water in copper sulfate pentahydrate crystals to one which incorporates students' prior knowledge, engenders active mental struggling with prior knowledge and new experiences, and encourages metacognition. (Contains 12 references.) (ASK)

LabVIEW Library to EPICS Channel Access

CERN Document Server

Liyu, Andrei; Thompson, Dave H

2005-01-01

The Spallation Neutron Source (SNS) accelerator systems will deliver a 1.0 GeV, 1.4 MW proton beam to a liquid mercury target for neutron scattering research. The accelerator complex consists of a 1 GeV linear accelerator, an accumulator ring and associated transport lines. The SNS diagnostics platform is PC-based and will run Windows for its OS and LabVIEW as its programming language. Data acquisition hardware will be based on PCI cards. There will be about 300 rack-mounted computers. The Channel Access (CA) protocol of the Experimental Physics and Industrial Control System (EPICS) is the SNS control system communication standard. This paper describes the approaches, implementation, and features of LabVIEW library to CA for Windows, Linux, and Mac OS X. We also discuss how the library implements the asynchronous CA monitor routine using LabVIEW's occurrence mechanism instead of a callback function (which is not available in LabVIEW). The library is used to acquire accelerator data and applications have been ...

Clothing Systems Design Lab

Data.gov (United States)

Federal Laboratory Consortium — The Clothing Systems Design Lab houses facilities for the design and rapid prototyping of military protective apparel.Other focuses include: creation of patterns and...

Tritium Assay and Dispensing of KEPRI Tritium Lab

International Nuclear Information System (INIS)

Sohn, S. H.; Song, K. M.; Lee, S. K.; Lee, K.W.; Ko, B. W.

2009-01-01

The Wolsong Tritium Removal Facility(WTRF) has been constructed to reduce tritium levels in the heavy water systems and environmental emissions at the site. The WTRF was designed to process 100 kg/h of heavy water with the overall tritium extraction efficiency of 97% per single pass and to produce ∼700 g of tritium as T2 per year at the feed concentration of 0.37 TBq/kg. The high purity tritium greater than 99% is immobilized as a metal hydride to secure its long term storage. The recovered tritium will be made available for industrial uses and some research applications in the future. Then KEPRI is constructing the tritium lab. to build-up infrastructure to support tritium research activities and to support tritium control and accountability systems for tritium export. This paper describes the initial phases of the tritium application program including the laboratory infrastructure to support the tritium related R and D activities and the tritium controls in Korea

Glycan elongation beyond the mucin associated Tn antigen protects tumor cells from immune-mediated killing.

Directory of Open Access Journals (Sweden)

Caroline B Madsen

Full Text Available Membrane bound mucins are up-regulated and aberrantly glycosylated during malignant transformation in many cancer cells. This results in a negatively charged glycoprotein coat which may protect cancer cells from immune surveillance. However, only limited data have so far demonstrated the critical steps in glycan elongation that make aberrantly glycosylated mucins affect the interaction between cancer cells and cytotoxic effector cells of the immune system. Tn (GalNAc-Ser/Thr, STn (NeuAcα2-6GalNAc-Ser/Thr, T (Galβ1-3GalNAc-Ser/Thr, and ST (NeuAcα2-6Galβ1-3GalNAc-Ser/Thr antigens are recognized as cancer associated truncated glycans, and are expressed in many adenocarcinomas, e.g. breast- and pancreatic cancer cells. To investigate the role of the cancer associated glycan truncations in immune-mediated killing we created glyco-engineered breast- and pancreatic cancer cells expressing only the shortest possible mucin-like glycans (Tn and STn. Glyco-engineering was performed by zinc finger nuclease (ZFN knockout (KO of the Core 1 enzyme chaperone COSMC, thereby preventing glycan elongation beyond the initial GalNAc residue in O-linked glycans. We find that COSMC KO in the breast and pancreatic cancer cell lines T47D and Capan-1 increases sensitivity to both NK cell mediated antibody-dependent cellular-cytotoxicity (ADCC and cytotoxic T lymphocyte (CTL-mediated killing. In addition, we investigated the association between total cell surface expression of MUC1/MUC16 and NK or CTL mediated killing, and observed an inverse correlation between MUC16/MUC1 expression and the sensitivity to ADCC and CTL-mediated killing. Together, these data suggest that up-regulation of membrane bound mucins protects cells from immune mediated killing, and that particular glycosylation steps, as demonstrated for glycan elongation beyond Tn and STn, can be important for fine tuning of the immune escape mechanisms in cancer cells.

LIDAR Research & Development Lab

Data.gov (United States)

Federal Laboratory Consortium — The LIDAR Research and Development labs are used to investigate and improve LIDAR components such as laser sources, optical signal detectors and optical filters. The...

German lab wins linear collider contest

CERN Multimedia

Cartlidge, Edwin

2004-01-01

Particle physicists have chosen to base the proposed International Linear Collider on superconducting technology developed by an international collaboration centred on the DESY lab in Germany. The superconducting approach was chosen by an internatinal panel ahead of a rival technology developed at Stanford in the US and the KEK lab in Japan. The eagerly-awaited decision was announced at the International Conference on High Energy Physics in Beijing today (½ page)

Fifteen years experience: Egyptian metabolic lab

Directory of Open Access Journals (Sweden)

Ekram M. Fateen

2014-10-01

Conclusion: This study illustrates the experience of the reference metabolic lab in Egypt over 15 years. The lab began metabolic disorder screening by using simple diagnostic techniques like thin layer chromatography and colored tests in urine which by time updated and upgraded the methods to diagnose a wide range of disorders. This study shows the most common diagnosed inherited inborn errors of metabolism among the Egyptian population.

S'Cool LAB Summer CAMP 2017

CERN Multimedia

Woithe, Julia

2017-01-01

The S’Cool LAB Summer CAMP is an opportunity for high-school students (aged 16-19) from all around the world to spend 2 weeks exploring the fascinating world of particle physics. The 24 selected participants spend their summer at S’Cool LAB, CERN’s hands-on particle physics learning laboratory, for an epic programme of lectures and tutorials, team research projects, visits of CERN’s research installations, and social activities.

CERN Technical Training 2006: LabVIEW Course Sessions (September-December 2006)

CERN Multimedia

2006-01-01

The following LabVIEW course sessions are currently scheduled in the framework of the CERN Technical Training Programme 2006, and in collaboration with National Instruments (CH): LabVIEW Basics 1 (course in English): 11-13.9.2006 (3 days, only 3 places available) (course in English): 14-15.9.2006 (2 days) LabVIEW: Working efficiently with LabVIEW 8 (course in English): 18.9.2006 (1 day) **NEW COURSE** LabVIEW Application Development (course in English): 13-15.11.2006 (3 days. Pre-requisite: LabVIEW Basics I ans II, or equivalent experience) LabVIEW Advanced Programming (course in English): 16-17.11.2006 (2 days. Pre-requisite: LabVIEW Application Development, or equivalent experience) LabVIEW Base 1 (course in French): 4-6.12.2006 (3 days, only 1 place available) LabVIEW Base 2 (course in French): 7-8.12.2006 (2 days) If you are interested in attending any of the above course sessions, please discuss with your supervisor and/or your DTO,...

The use of tetragnathid spiders as bioindicators of metal exposure at a coal ash spill site.

Science.gov (United States)

Otter, Ryan R; Hayden, Mary; Mathews, Teresa; Fortner, Allison; Bailey, Frank C

2013-09-01

On 22 December 2008, a dike containing coal fly ash from the Tennessee Valley Authority Kingston Fossil Fuel Plant (TN, USA) failed, resulting in the largest coal ash spill in US history. The present study was designed to determine sediment metal concentrations at multiple site locations and to determine whether site-specific bioaccumulation of metals existed in tetragnathid spiders. Selenium and nickel were the only 2 metals to exceed the US Environmental Protection Agency sediment screening levels. Selenium concentrations in spiders were significantly higher at ash-affected sites than in those from reference sites. The ratio of methylmercury to total mercury in spiders was found to be similar to that in other organisms (65-75%), which highlights the potential use of tetragnathid spiders as an indicator species for tracing contaminant transfer between the aquatic and terrestrial ecosystems. Copyright © 2013 SETAC.

Reference-based pricing: an evidence-based solution for lab services shopping.

Science.gov (United States)

Melton, L Doug; Bradley, Kent; Fu, Patricia Lin; Armata, Raegan; Parr, James B

2014-01-01

To determine the effect of reference-based pricing (RBP) on the percentage of lab services utilized by members that were at or below the reference price. Retrospective, quasi-experimental, matched, case-control pilot evaluation of an RBP benefit for lab services. The study group included employees of a multinational grocery chain covered by a national health insurance carrier and subject to RBP for lab services; it had access to an online lab shopping tool and was informed about the RBP benefit through employer communications. The reference group was covered by the same insurance carrier but not subject to RBP. The primary end point was lab compliance, defined as the percentage of lab claims with total charges at or below the reference price. Difference-in-difference regression estimation evaluated changes in lab compliance between the 2 groups. Higher compliance per lab claim was evident for the study group compared with the reference group (69% vs 57%; Ponline shopping tool was used by 7% of the matched-adjusted study group prior to obtaining lab services. Lab compliance was 76% for study group members using the online tool compared with 68% among nonusers who were subject to RBP (P<.01). RBP can promote cost-conscious selection of lab services. Access to facilities that offer services below the reference price and education about RBP improve compliance. Evaluation of the effect of RBP on higher-cost medical services, including radiology, outpatient specialty, and elective inpatient procedures, is needed.

A Well-Maintained Lab Is a Safer Lab. Safety Spotlight

Science.gov (United States)

Walls, William H.; Strimel, Greg J.

2018-01-01

Administration and funding can cause Engineering/Technology Education (ETE) programs to thrive or die. To administrators, the production/prototyping equipment and laboratory setting are often viewed as the features that set ETE apart from other school subjects. A lab is a unique gift as well as a responsibility. If an administrator can see that…

Heavy metal oxide glasses as gamma rays shielding material

International Nuclear Information System (INIS)

Kaur, Preet; Singh, Devinder; Singh, Tejbir

2016-01-01

The gamma rays shielding parameters for heavy metal oxide glasses and concrete samples are comparable. However, the transparent nature of glasses provides additional feature to visualize inside the shielding material. Hence, different researchers had contributed in computing/measuring different shielding parameters for different configurations of heavy metal oxide glass systems. In the present work, a detailed study on different heavy metal (_5_6Ba, _6_4Gd, _8_2Pb, _8_3Bi) oxide glasses has been presented on the basis of different gamma rays shielding parameters as reported by different researchers in the recent years. It has been observed that among the selected heavy metal oxide glass systems, Bismuth based glasses provide better gamma rays shielding. Hence, Bismuth based glasses can be better substitute to concrete walls at nuclear reactor sites and nuclear labs.

Heavy metal oxide glasses as gamma rays shielding material

Energy Technology Data Exchange (ETDEWEB)

Kaur, Preet; Singh, Devinder; Singh, Tejbir, E-mail: dr.tejbir@gmail.com

2016-10-15

The gamma rays shielding parameters for heavy metal oxide glasses and concrete samples are comparable. However, the transparent nature of glasses provides additional feature to visualize inside the shielding material. Hence, different researchers had contributed in computing/measuring different shielding parameters for different configurations of heavy metal oxide glass systems. In the present work, a detailed study on different heavy metal ({sub 56}Ba, {sub 64}Gd, {sub 82}Pb, {sub 83}Bi) oxide glasses has been presented on the basis of different gamma rays shielding parameters as reported by different researchers in the recent years. It has been observed that among the selected heavy metal oxide glass systems, Bismuth based glasses provide better gamma rays shielding. Hence, Bismuth based glasses can be better substitute to concrete walls at nuclear reactor sites and nuclear labs.

MatLab Programming for Engineers Having No Formal Programming Knowledge

Science.gov (United States)

Shaykhian, Linda H.; Shaykhian, Gholam Ali

2007-01-01

MatLab is one of the most widely used very high level programming languages for Scientific and engineering computations. It is very user-friendly and needs practically no formal programming knowledge. Presented here are MatLab programming aspects and not just the MatLab commands for scientists and engineers who do not have formal programming training and also have no significant time to spare for learning programming to solve their real world problems. Specifically provided are programs for visualization. Also, stated are the current limitations of the MatLab, which possibly can be taken care of by Mathworks Inc. in a future version to make MatLab more versatile.

eComLab: remote laboratory platform

Science.gov (United States)

Pontual, Murillo; Melkonyan, Arsen; Gampe, Andreas; Huang, Grant; Akopian, David

2011-06-01

Hands-on experiments with electronic devices have been recognized as an important element in the field of engineering to help students get familiar with theoretical concepts and practical tasks. The continuing increase the student number, costly laboratory equipment, and laboratory maintenance slow down the physical lab efficiency. As information technology continues to evolve, the Internet has become a common media in modern education. Internetbased remote laboratory can solve a lot of restrictions, providing hands-on training as they can be flexible in time and the same equipment can be shared between different students. This article describes an on-going remote hands-on experimental radio modulation, network and mobile applications lab project "eComLab". Its main component is a remote laboratory infrastructure and server management system featuring various online media familiar with modern students, such as chat rooms and video streaming.

Production of diketopiperazine derivative cyclo (l-leu-l-arg) by streptomyces sp. tn262 after exposure to heat-killed fungus fusarium sp

International Nuclear Information System (INIS)

Elleuch, L.; Smaoui, S.; Najah, S.; Sellem, I

2013-01-01

In a screening program for new active secondary metabolites producers, a strain of Streptomyces called TN262 was isolated from Tunisian soil and selected for its ability to produce eleven active compounds in pure culture conditions. In this work, the effect of different concentrations of heat-killed fungus Fusarium sp. on the production of active compounds by TN262 strain was studied. The ethyl acetate extract from the culture of Streptomyces sp. TN262 combined with heat-killed Fusarium sp. at 50 micro g/ml inhibited the growth of the three used indicator microorganisms. In fact, an increase of 36%, 21% and 20% in inhibitory activity was obtained against Micrococcus luteus LB 14110, Escherichia coli ATCC 8739 and Fusarium sp. respectively. The HPLC chromatographic profiles of the ethyl acetate extracts from both culture conditions were different and an additional active compound was produced only under induced conditions. This active component was isolated and identified as Cyclo (L-Leu-L-Arg) (1), a diketopiperazine derivative, possessing antibacterial and antifungal activity. Consequently, this study showed that the addition of heat-killed fungus is a useful method for inducing the production of bioactive compounds. (author)

Technology Roadmap: Lab-on-a-Chip

OpenAIRE

Pattharaporn Suntharasaj; Tugrul U Daim

2010-01-01

With the integration of microfluidic and MEMS technologies, biochips such as the lab-on-a-chip (LOC) devices are at the brink of revolutionizing the medical disease diagnostics industries. Remarkable advancements in the biochips industry are making products resembling Star Trek.s "tricorder" and handheld medical scanners a reality. Soon, doctors can screen for cancer at the molecular level without costly and cumbersome equipments, and discuss treatment plans based on immediate lab results. Th...

LXI Technologies for Remote Labs: An Extension of the VISIR Project

OpenAIRE

Jaime Irurzun; Olga Dziabenko; Pablo Orduña; Diego Lopez-de-Ipiña; Ignacio Angulo; Javier García-Zubia; Unai Hernandez-Jayo

2010-01-01

Several remote labs to support analog circuits are presented in this work. They are analyzed from the software and the hardware point of view. VISIR remote lab is one of these labs. After this analysis, a new VISIR remote lab approach is presented. This extension of the VISIR project is based on LXI technologies with the aim of becoming it in a remote lab easily interchangeable with other instruments. The addition of new components and experiments is also easier and cheaper.

Reducing unnecessary lab testing in the ICU with artificial intelligence.

Science.gov (United States)

Cismondi, F; Celi, L A; Fialho, A S; Vieira, S M; Reti, S R; Sousa, J M C; Finkelstein, S N

2013-05-01

To reduce unnecessary lab testing by predicting when a proposed future lab test is likely to contribute information gain and thereby influence clinical management in patients with gastrointestinal bleeding. Recent studies have demonstrated that frequent laboratory testing does not necessarily relate to better outcomes. Data preprocessing, feature selection, and classification were performed and an artificial intelligence tool, fuzzy modeling, was used to identify lab tests that do not contribute an information gain. There were 11 input variables in total. Ten of these were derived from bedside monitor trends heart rate, oxygen saturation, respiratory rate, temperature, blood pressure, and urine collections, as well as infusion products and transfusions. The final input variable was a previous value from one of the eight lab tests being predicted: calcium, PTT, hematocrit, fibrinogen, lactate, platelets, INR and hemoglobin. The outcome for each test was a binary framework defining whether a test result contributed information gain or not. Predictive modeling was applied to recognize unnecessary lab tests in a real world ICU database extract comprising 746 patients with gastrointestinal bleeding. Classification accuracy of necessary and unnecessary lab tests of greater than 80% was achieved for all eight lab tests. Sensitivity and specificity were satisfactory for all the outcomes. An average reduction of 50% of the lab tests was obtained. This is an improvement from previously reported similar studies with average performance 37% by [1-3]. Reducing frequent lab testing and the potential clinical and financial implications are an important issue in intensive care. In this work we present an artificial intelligence method to predict the benefit of proposed future laboratory tests. Using ICU data from 746 patients with gastrointestinal bleeding, and eleven measurements, we demonstrate high accuracy in predicting the likely information to be gained from proposed future

Reducing unnecessary lab testing in the ICU with artificial intelligence

Science.gov (United States)

Cismondi, F.; Celi, L.A.; Fialho, A.S.; Vieira, S.M.; Reti, S.R.; Sousa, J.M.C.; Finkelstein, S.N.

2017-01-01

Objectives To reduce unnecessary lab testing by predicting when a proposed future lab test is likely to contribute information gain and thereby influence clinical management in patients with gastrointestinal bleeding. Recent studies have demonstrated that frequent laboratory testing does not necessarily relate to better outcomes. Design Data preprocessing, feature selection, and classification were performed and an artificial intelligence tool, fuzzy modeling, was used to identify lab tests that do not contribute an information gain. There were 11 input variables in total. Ten of these were derived from bedside monitor trends heart rate, oxygen saturation, respiratory rate, temperature, blood pressure, and urine collections, as well as infusion products and transfusions. The final input variable was a previous value from one of the eight lab tests being predicted: calcium, PTT, hematocrit, fibrinogen, lactate, platelets, INR and hemoglobin. The outcome for each test was a binary framework defining whether a test result contributed information gain or not. Patients Predictive modeling was applied to recognize unnecessary lab tests in a real world ICU database extract comprising 746 patients with gastrointestinal bleeding. Main results Classification accuracy of necessary and unnecessary lab tests of greater than 80% was achieved for all eight lab tests. Sensitivity and specificity were satisfactory for all the outcomes. An average reduction of 50% of the lab tests was obtained. This is an improvement from previously reported similar studies with average performance 37% by [1–3]. Conclusions Reducing frequent lab testing and the potential clinical and financial implications are an important issue in intensive care. In this work we present an artificial intelligence method to predict the benefit of proposed future laboratory tests. Using ICU data from 746 patients with gastrointestinal bleeding, and eleven measurements, we demonstrate high accuracy in predicting the

PTH Test

Science.gov (United States)

... Gregory C. Sephel PhD FACB MT(ASCP). Lab Tests Online adjunct board member. Director Clinical Pathology, VA TN Valley Healthcare System; Associate Professor Pathology, Microbiology, Immunology, Vanderbilt University School of Medicine. Sources Used ...

Release to the gas phase of metals, S and Cl during combustion of dedicated waste fractions

DEFF Research Database (Denmark)

Pedersen, Anne Juul; van Lith, Simone Cornelia; Frandsen, Flemming

2010-01-01

The release to the gas phase of inorganic elements such as alkali metals. Cl, S, and heavy metals in Waste-to-Energy (WtE) boilers is a challenge. Besides the risk of harmful emissions to the environment, inorganic elements released from the grate may cause severe ash deposition and corrosion...... and the link to the formation of fly ash and aerosols in full-scale waste incinerators. The release of metals, S and Cl from four dedicated waste fractions was quantified as a function of temperature in a lab-scale fixed-bed reactor. The waste fractions comprised chromated copper arsenate (CCA) impregnated....... The lab-scale release results were then compared with results from a related, full-scale partitioning study, in which test runs with the addition of similar, dedicated waste fractions to a base-load waste had been performed in a grate-fired WtE boiler. In general, the elements Al, Ca, Cr, Cu, Fe, Mg, Si...

Overview of RepLab 2012: Evaluating Online Reputation Management Systems

NARCIS (Netherlands)

Amigó, E.; Corujo, A.; Gonzalo, J.; Meij, E.; de Rijke, M.

2012-01-01

This paper summarizes the goals, organization and results of the first RepLab competitive evaluation campaign for Online Reputation Management Systems (RepLab 2012). RepLab focused on the reputation of companies, and asked participant systems to annotate different types of information on tweets

LXI Technologies for Remote Labs: An Extension of the VISIR Project

Directory of Open Access Journals (Sweden)

Jaime Irurzun

2010-09-01

Full Text Available Several remote labs to support analog circuits are presented in this work. They are analyzed from the software and the hardware point of view. VISIR remote lab is one of these labs. After this analysis, a new VISIR remote lab approach is presented. This extension of the VISIR project is based on LXI technologies with the aim of becoming it in a remote lab easily interchangeable with other instruments. The addition of new components and experiments is also easier and cheaper.

Integrating Multiple On-line Knowledge Bases for Disease-Lab Test Relation Extraction.

Science.gov (United States)

Zhang, Yaoyun; Soysal, Ergin; Moon, Sungrim; Wang, Jingqi; Tao, Cui; Xu, Hua

2015-01-01

A computable knowledge base containing relations between diseases and lab tests would be a great resource for many biomedical informatics applications. This paper describes our initial step towards establishing a comprehensive knowledge base of disease and lab tests relations utilizing three public on-line resources. LabTestsOnline, MedlinePlus and Wikipedia are integrated to create a freely available, computable disease-lab test knowledgebase. Disease and lab test concepts are identified using MetaMap and relations between diseases and lab tests are determined based on source-specific rules. Experimental results demonstrate a high precision for relation extraction, with Wikipedia achieving the highest precision of 87%. Combining the three sources reached a recall of 51.40%, when compared with a subset of disease-lab test relations extracted from a reference book. Moreover, we found additional disease-lab test relations from on-line resources, indicating they are complementary to existing reference books for building a comprehensive disease and lab test relation knowledge base.

The experiment editor: supporting inquiry-based learning with virtual labs

Science.gov (United States)

Galan, D.; Heradio, R.; de la Torre, L.; Dormido, S.; Esquembre, F.

2017-05-01

Inquiry-based learning is a pedagogical approach where students are motivated to pose their own questions when facing problems or scenarios. In physics learning, students are turned into scientists who carry out experiments, collect and analyze data, formulate and evaluate hypotheses, and so on. Lab experimentation is essential for inquiry-based learning, yet there is a drawback with traditional hands-on labs in the high costs associated with equipment, space, and maintenance staff. Virtual laboratories are helpful to reduce these costs. This paper enriches the virtual lab ecosystem by providing an integrated environment to automate experimentation tasks. In particular, our environment supports: (i) scripting and running experiments on virtual labs, and (ii) collecting and analyzing data from the experiments. The current implementation of our environment supports virtual labs created with the authoring tool Easy Java/Javascript Simulations. Since there are public repositories with hundreds of freely available labs created with this tool, the potential applicability to our environment is considerable.

77 FR 51546 - Federal Property Suitable as Facilities To Assist the Homeless

Science.gov (United States)

2012-08-24

... Number: 4-B-TN-0664-AA Comments: 15,955 sq. ft., most recent use--office/storage/lab Virginia Hampton Rds... Program Nevada RBG Water Project Site Bureau of Reclamation Henderson NV 89011 Landholding Agency: GSA...

Hydroscoop - Bulletin of the small-scale hydraulic laboratory MHyLab; Hydroscoop - Bulletin d'information MHyLab laboratoire de petite hydraulique

Energy Technology Data Exchange (ETDEWEB)

Denis, V.

2009-07-01

This is issue Nr. 5 of the news bulletin of MHyLab, the small-scale hydraulic laboratory in Montcherand, Switzerland. The history of MHyLab development is recalled. The objective of the laboratory is given: the laboratory development of efficient and reliable turbines for the entire small-scale hydraulic range (power: 10 to 2000 kW, flow rate: 0.01 to 10 m{sup 3}/s, hydraulic head: 1 m up to more than 700 m). The first period (1997-2001) was devoted to Pelton turbines for high heads (60 to 70 m) and the second (2001-2009) to Kaplan turbines for low and very low heads (1 to 30 m). In the third period (beginning 2008) diagonal turbines for medium heads (25 to 100 m) are being developed. MHyLab designed, modelled and tested all these different types. The small-scale hydraulic market developed unexpectedly quickly. The potential of small-scale hydraulics in the Canton of Vaud, western Switzerland is presented. Three implemented projects are reported on as examples for MHyLab activities on the market place. The MHyLab staff is presented.

High-Level Heat Resistance of Spores of Bacillus amyloliquefaciens and Bacillus licheniformis Results from the Presence of a spoVA Operon in a Tn1546 Transposon

Science.gov (United States)

Berendsen, Erwin M.; Koning, Rosella A.; Boekhorst, Jos; de Jong, Anne; Kuipers, Oscar P.; Wells-Bennik, Marjon H. J.

2016-01-01

Bacterial endospore formers can produce spores that are resistant to many food processing conditions, including heat. Some spores may survive heating processes aimed at production of commercially sterile foods. Recently, it was shown that a spoVA operon, designated spoVA2mob, present on a Tn1546 transposon in Bacillus subtilis, leads to profoundly increased wet heat resistance of B. subtilis spores. Such Tn1546 transposon elements including the spoVA2mob operon were also found in several strains of Bacillus amyloliquefaciens and Bacillus licheniformis, and these strains were shown to produce spores with significantly higher resistances to wet heat than their counterparts lacking this transposon. In this study, the locations and compositions of Tn1546 transposons encompassing the spoVA2mob operons in B. amyloliquefaciens and B. licheniformis were analyzed. Introduction of these spoVA2mob operons into B. subtilis 168 (producing spores that are not highly heat resistant) rendered mutant 168 strains that produced high-level heat resistant spores, demonstrating that these elements in B. amyloliquefaciens and B. licheniformis are responsible for high level heat resistance of spores. Assessment of growth of the nine strains of each species between 5.2°C and 57.7°C showed some differences between strains, especially at lower temperatures, but all strains were able to grow at 57.7°C. Strains of B. amyloliquefaciens and B. licheniformis that contain the Tn1546 elements (and produce high-level heat resistant spores) grew at temperatures similar to those of their Tn1546-negative counterparts that produce low-level heat resistant spores. The findings presented in this study allow for detection of B. amyloliquefaciens and B. licheniformis strains that produce highly heat resistant spores in the food chain. PMID:27994575

Experimental investigation of new manufacturing process chains to create micro-metal structures on polymer substrates for lab-on-chip sensors

DEFF Research Database (Denmark)

Calaon, Matteo; Islam, Aminul; Hansen, Hans Nørgaard

2012-01-01

Over the last two decades, lab-on-a-chip devices have emerged as a leading technology for life sciences, drug development, medical diagnostics, food safety, agricultural and environmental monitoring. The conventional methods used nowadays to manufacture these micro- and nano-functional surface...

Modeling Adsorption Kinetics (Bio-remediation of Heavy Metal Contaminated Water)

Science.gov (United States)

McCarthy, Chris

My talk will focus on modeling the kinetics of the adsorption and filtering process using differential equations, stochastic methods, and recursive functions. The models have been developed in support of our interdisciplinary lab group which is conducting research into bio-remediation of heavy metal contaminated water via filtration through biomass such as spent tea leaves. The spent tea leaves are available in large quantities as a result of the industrial production of tea beverages. The heavy metals bond with the surfaces of the tea leaves (adsorption). Funding: CUNY Collaborative Incentive Research Grant.

A Case Study of a High School Fab Lab

Science.gov (United States)

Lacy, Jennifer E.

This dissertation examines making and design-based STEM education in a formal makerspace. It focuses on how the design and implementation of a Fab Lab learning environment and curriculum affect how instructors and students see themselves engaging in science, and how the Fab Lab relates to the social sorting practices that already take place at North High School. While there is research examining design-based STEM education in informal and formal learning environments, we know little about how K-12 teachers define STEM in making activities when no university or museum partnership exists. This study sought to help fill this gap in the research literature. This case study of a formal makerspace followed instructors and students in one introductory Fab Lab course for one semester. Additional observations of an introductory woodworking course helped build the case and set it into the school context, and provided supplementary material to better understand the similarities and differences between the Fab Lab course and a more traditional design-based learning course. Using evidence from observational field notes, participant interviews, course materials, and student work, I found that the North Fab Lab relies on artifacts and rhetoric symbolic of science and STEM to set itself apart from other design-based courses at North High School. Secondly, the North Fab Lab instructors and students were unable to explain how what they were doing in the Fab Lab was science, and instead relied on vague and unsupported claims related to interdisciplinary STEM practices and dated descriptions of science. Lastly, the design and implementation of the Fab Lab learning environment and curriculum and its separation from North High School's low tech, design-based courses effectively reinforced social sorting practices and cultural assumptions about student work and intelligence.

Application of LabVIEW on Ionization Chamber to Measurement Radiation

International Nuclear Information System (INIS)

Kerdchockchai, P.; Soodprasert, T.; Hoonnivathana, E.; Naemchnthara, P.; Limsuwan, P.; Naemchanthara, K.

2014-01-01

The purpose of this research was to apply LabVIEW program to control an ionization chamber. LabVIEW was used to compose a block diagram and front panel. The block diagram was programmed to be controlled by the front panel. Radiation dose of Cs -137 at 1.00, 1.50, 2.00, 2.50, 3.00 and 4.00 meter were compared from LabViEW and manual system. The results show that the different percentages of Pb filter of thickness 0, 20 and 39 mm are 0.68, 0.68 and 0.48, respectively. This experiment results indicated that the LabVIEW can be used in assisting radiation measurement. Furthermore, by controlling the ionization chamber by LabVIEW, the radiation dose received by operator is reduced.

A Low-Cost Remote Lab for Internet Services Distance Education

Directory of Open Access Journals (Sweden)

James Sissom

2006-08-01

Full Text Available Academic departments seeking to reach students via distance education course offerings find that some on-line curricula require a traditional hands-on lab model for student evaluation and assessment. The authors solve the problem of providing distance education curriculum and supporting instruction lab components by using a low-cost remote lab. The remote lab is used to evaluate student performance in managing web services and website development, solving security problems, patch management, scripting and web server management. In addition, the authors discuss assessment and evaluation techniques that will be used to determine instructional quality and student performance. Discussed are the remote lab architecture, use of disk images and utilization of Windows 2003 Internet Information Service, and Linux Red Hat 9.0 platforms.

The History of Science and Technology at Bell Labs

Science.gov (United States)

Bishop, David

2008-03-01

Over the last 80 years, Bell Labs has been one of the most scientifically and technologically productive research labs in the world. Inventions such as the transistor, laser, cell phone, solar cell, negative feedback amplifier, communications satellite and many others were made there. Scientific breakthroughs such as discovery of the Big Bang, the wave nature of the electron, electron localization and the fractional quantum hall effect were also made there making Bell Labs almost unique in terms of large impacts in both science and technology. In my talk, I will discuss the history of the lab, talk about the present and give some suggestions for how I see it evolving into the future.

IHF-independent assembly of the Tn10 strand transfer transpososome: implications for inhibition of disintegration

OpenAIRE

Stewart, Barry J.; Wardle, Simon J.; Haniford, David B.

2002-01-01

The frequency of DNA transposition in transposition systems that employ a strand transfer step may be significantly affected by the occurrence of a disintegration reaction, a reaction that reverses the strand transfer event. We have asked whether disintegration occurs in the Tn10 transposition system. We show that disintegration substrates (substrates constituting one half of the strand transfer product) are assembled into a transpososome that mimics the strand transfer intermediate. This str...

Time Trials--An AP Physics Challenge Lab

Science.gov (United States)

Jones, David

2009-01-01

I have come to the conclusion that for high school physics classroom and laboratory experiences, simpler is better! In this paper I describe a very simple and effective lab experience that my AP students have thoroughly enjoyed year after year. I call this lab exercise "Time Trials." The experiment is simple in design and it is a lot of fun for…

An overview of the user program for the Jefferson Lab free electron laser

International Nuclear Information System (INIS)

Dylla, H.F.

1999-01-01

Jefferson Lab is commissioning a high-average-power IR FEL during 1998. When driven with its superconducting linac operating in a recirculated mode, the IR Demo FEL is capable of producing kilowatt-level average power in the mid-infrared (2-7 mu m) range. With operational experience and hardware changes involving primarily change-out of the optical cavity mirrors, the FEL is capable of covering a wide range of the infrared (1-16 mu m) at power levels exceeding 100 W. This tuning range combined with a unique pulse structure makes the Jefferson Lab FEL a versatile research and development tool for a wide variety of laser applications. A core group of industrial partners has been involved in planning applications using the FEL since 1991. This initial user group was augmented with university partners in 1993 and with participants from several national laboratories in 1996-1997. With the initiation of construction of the FEL and the associated 600 m 2 user facility laboratory in 1996, a number of topical user groups were formed to plan and implement the first series of user experiments. The industrial partners have formed user groups planning applications in polymer surface processing, metal surface processing, microfabrication, and electronic materials. University partners have submitted proposals on basic science topics which complement and planned applied research topics, in addition to proposing experiments in atomic physics, chemical physics and materials science which take advantage of one or more of the unique characteristics of the FEL. A synopsis of the proposed user experiments for the first phase of operation of the Jefferson Lab FEL will be presented

Refractory metal component technology for in-core sensor design

International Nuclear Information System (INIS)

Cannon, C.P.

1986-02-01

Within recent years, an increasing concern over reactor safety has prompted tests that characterize reactor core environments during transient conditions. Such tests include the Loss-of-Fluid-Tests (Idaho National Engineering Lab (INEL)), Severe Fuel Damage Tests (INEL), Core Debris Rubble Tests (Sandia National Laboratories (SNL)), and similar tests performed by foreign nations. The in-core sensors for these tests require refractory metal components to be compatible with electrical insulator materials as well as materials comprising highly corrosive service mediums. This paper presents the refractory metal technology utilized to provide basic sensor designs in the above mentioned reactor tests

European labs brace for German cuts: international collaboration

CERN Multimedia

Clery, D

1996-01-01

Germany, the largest contributor to international European research labs, announced plans to reduce its contributions an average of 8% in the nation's latest budget. CERN and other labs are worried that the cuts will endanger ongoing projects and that other countries may follow Germany's lead.

Can Graduate Teaching Assistants Teach Inquiry-Based Geology Labs Effectively?

Science.gov (United States)

Ryker, Katherine; McConnell, David

2014-01-01

This study examines the implementation of teaching strategies by graduate teaching assistants (GTAs) in inquiry-based introductory geology labs at a large research university. We assess the degree of inquiry present in each Physical Geology lab and compare and contrast the instructional practices of new and experienced GTAs teaching these labs. We…

Constructing the Components of a Lab Report Using Peer Review

Science.gov (United States)

Berry, David E.; Fawkes, Kelli L.

2010-01-01

A protocol that emphasizes lab report writing using a piecemeal approach coupled with peer review is described. As the lab course progresses, the focus of the report writing changes sequentially through the abstract and introduction, the discussion, and the procedure. Two styles of lab programs are presented. One style rotates the students through…

Increasing Students’ Interest by Encouraging them to Create Original Lab Projects

Directory of Open Access Journals (Sweden)

Petre Lucian Ogrutan

2017-11-01

Full Text Available Sometimes traditional lab projects based on standard kits and modules fail to stimulate students’ interest and creativity. This paper presents a novel laboratory concept which allows students to develop their own lab projects using open-source resources. The lab experiment includes competition aspects allowing every student to come up with ideas of which the best are selected. The lab projects include both hard and software components using Arduino-compatible systems and interfaces. Before starting the practical activities as well as after the completion of the lab session, the students were asked to fill in an anonymous questionnaire.

Open web system of Virtual labs for nuclear and applied physics

International Nuclear Information System (INIS)

Saldikov, I S; Afanasyev, V V; Petrov, V I; Ternovykh, M Yu

2017-01-01

An example of virtual lab work on unique experimental equipment is presented. The virtual lab work is software based on a model of real equipment. Virtual labs can be used for educational process in nuclear safety and analysis field. As an example it includes the virtual lab called “Experimental determination of the material parameter depending on the pitch of a uranium-water lattice”. This paper included general description of this lab. A description of a database on the support of laboratory work on unique experimental equipment which is included this work, its concept development are also presented. (paper)

A Hardware Lab Anywhere At Any Time

Directory of Open Access Journals (Sweden)

Tobias Schubert

2004-12-01

Full Text Available Scientific technical courses are an important component in any student's education. These courses are usually characterised by the fact that the students execute experiments in special laboratories. This leads to extremely high costs and a reduction in the maximum number of possible participants. From this traditional point of view, it doesn't seem possible to realise the concepts of a Virtual University in the context of sophisticated technical courses since the students must be "on the spot". In this paper we introduce the so-called Mobile Hardware Lab which makes student participation possible at any time and from any place. This lab nevertheless transfers a feeling of being present in a laboratory. This is accomplished with a special Learning Management System in combination with hardware components which correspond to a fully equipped laboratory workstation that are lent out to the students for the duration of the lab. The experiments are performed and solved at home, then handed in electronically. Judging and marking are also both performed electronically. Since 2003 the Mobile Hardware Lab is now offered in a completely web based form.

Studies on the process of attachment of diazotroph alcaligenes faecalis and its Tn5 mutants to rice roots using 15N-labelling technique

International Nuclear Information System (INIS)

Fang Xuanjun; Lin Min; You Chongbiao

1993-09-01

By using 15 N-labelling technique and Tn5-induced mutants the attachment of associative diazotroph Alcaligenes faecalis to intact rice plants was examined in vitro. Three distinguished modes of attachment of Alcaligenes faecalis: adsorption, anchoring and colonization were proposed by using 15 N-labelling bacterial cells and Tn5-induced mutants. Che - mutants affected on adsorption, but not on anchoring. Exo - Che - mutant is defective in both adsorption and anchoring. Exo - or exo ++ mutants are only defective in anchoring. Effective colonization is benefit for establishment on the associative system. The data also indicated that EPS (exopolysaccharide) play rather important roles in the association between the host plant and bacteria

Selective Recovery of Metals from Geothermal Brines

Energy Technology Data Exchange (ETDEWEB)

Ventura, Susanna [SRI International, Menlo Park, CA (United States); Bhamidi, Srinivas [SRI International, Menlo Park, CA (United States); Hornbostel, Marc [SRI International, Menlo Park, CA (United States); Nagar, Anoop [SRI International, Menlo Park, CA (United States); Perea, Elisabeth [SRI International, Menlo Park, CA (United States)

2016-12-16

The objective of this project was to determine the feasibility of developing a new generation of highly selective low-cost ion-exchange resins based on metal-ion imprinted polymers for the separation of metals from geothermal fluids. Expansion of geothermal energy production over the entire U.S. will involve exploitation of low-to-medium temperature thermal waters. Creating value streams from the recovery of critical and near-critical metals from these thermal waters will encourage geothermal expansion. Selective extraction of metals from geothermal fluids is needed to design a cost-effective process for the recovery of lithium and manganese-two near-critical metals with well-known application in the growing lithium battery industry. We have prepared new lithium- and manganese-imprinted polymers in the form of beads by crosslinking polymerization of a metal polymerizable chelate, where the metal acts as a template. Upon leaching out the metal template, the crosslinked polymer is expected to leave cavities defined by the ligand functional group with enhanced selectivity for binding the template metal. We have demonstrated that lithium- and manganese-imprinted polymer beads can be used as selective solid sorbents for the extraction of lithium and manganese from brines. The polymers were tested both in batch extractions and packed bed lab-scale columns at temperatures of 45-100°C. Lithium-imprinted polymers were found to have Li⁺ adsorption capacity as high as 2.8 mg Li⁺/g polymer at 45°C. Manganese-imprinted polymers were found to have a Mn²⁺ adsorption capacity of more than 23 mg Mn²⁺/g polymer at 75°C. The Li⁺ extraction efficiency of the Li-imprinted polymer was found to be more that 95% when a brine containing 390 ppm Li⁺, 410 ppm Na⁺, and 390 ppm K⁺ was passed through a packed bed of the polymer in a lab-scale column at 45°C. In brines containing 360 ppm Li

A LabVIEWTM-based detector testing system

International Nuclear Information System (INIS)

Yang Haori; Li Yuanjing; Wang Yi; Li Yulan; Li Jin

2003-01-01

The construction of a LabVIEW-based detector testing system is described in this paper. In this system, the signal of detector is magnified and digitized, so amplitude or time spectrum can be obtained. The Analog-to-Digital Converter is a peak-sensitive ADC based on VME bus. The virtual instrument constructed by LabVIEW can be used to acquire data, draw spectrum and save testing results

Exact comparison of dose rate measurements and calculation of TN12/2 packages

International Nuclear Information System (INIS)

Taniuchi, H.; Matsuda, F.

1998-01-01

Both of dose rate measurements of TN 12/2 package and calculations by Monte Carlo code MORSE in SCALE code system and MCNP were performed to evaluate the difference between the measurement and the calculation and finding out the cause of the difference. The calculated gamma-ray dose rates agreed well with measured ones, but calculated neutron dose rates overestimated more than a factor of 1.7. When considering the cause of the difference and applying the modification into the neutron calculation, the calculated neutron dose rates become to agree well, and the factor decreased to around 1.3. (authors)

Physics lab in spin

CERN Multimedia

Hawkes, N

1999-01-01

RAL is fostering commerical exploitation of its research and facilities in two main ways : spin-out companies exploit work done at the lab, spin-in companies work on site taking advantage of the facilities and the expertise available (1/2 page).

Practical Clinical Training in Skills Labs: Theory and Practice

Directory of Open Access Journals (Sweden)

Bugaj, T. J.

2016-08-01

Full Text Available Today, skills laboratories or “skills labs”, i.e. specific practical skill training facilities, are a firmly established part of medical education offering the possibility of training clinical procedures in a safe and fault-forging environment prior to real life application at bedside or in the operating room. Skills lab training follows a structured teaching concept, takes place under supervision and in consideration of methodological-didactic concepts, ideally creating an atmosphere that allows the repeated, anxiety- and risk-free practice of targeted skills.In this selective literature review, the first section is devoted to (I the development and dissemination of the skills lab concept. There follows (II an outline of the underlying idea and (III an analysis of key efficacy factors. Thereafter, (IV the training method’s effectiveness and transference are illuminated, before (V the use of student tutors, in the sense of peer-assisted-learning, in skills labs is discussed separately. Finally, (VI the efficiency of the skills lab concept is analyzed, followed by an outlook on future developments and trends in the field of skills lab training.

LabVIEW Interface for PCI-SpaceWire Interface Card

Science.gov (United States)

Lux, James; Loya, Frank; Bachmann, Alex

2005-01-01

This software provides a LabView interface to the NT drivers for the PCISpaceWire card, which is a peripheral component interface (PCI) bus interface that conforms to the IEEE-1355/ SpaceWire standard. As SpaceWire grows in popularity, the ability to use SpaceWire links within LabVIEW will be important to electronic ground support equipment vendors. In addition, there is a need for a high-level LabVIEW interface to the low-level device- driver software supplied with the card. The LabVIEW virtual instrument (VI) provides graphical interfaces to support all (1) SpaceWire link functions, including message handling and routing; (2) monitoring as a passive tap using specialized hardware; and (3) low-level access to satellite mission-control subsystem functions. The software is supplied in a zip file that contains LabVIEW VI files, which provide various functions of the PCI-SpaceWire card, as well as higher-link-level functions. The VIs are suitably named according to the matching function names in the driver manual. A number of test programs also are provided to exercise various functions.

EarthLabs - Investigating Hurricanes: Earth's Meteorological Monsters

Science.gov (United States)

McDaris, J. R.; Dahlman, L.; Barstow, D.

2007-12-01

Earth science is one of the most important tools that the global community needs to address the pressing environmental, social, and economic issues of our time. While, at times considered a second-rate science at the high school level, it is currently undergoing a major revolution in the depth of content and pedagogical vitality. As part of this revolution, labs in Earth science courses need to shift their focus from cookbook-like activities with known outcomes to open-ended investigations that challenge students to think, explore and apply their learning. We need to establish a new model for Earth science as a rigorous lab science in policy, perception, and reality. As a concerted response to this need, five states, a coalition of scientists and educators, and an experienced curriculum team are creating a national model for a lab-based high school Earth science course named EarthLabs. This lab course will comply with the National Science Education Standards as well as the states' curriculum frameworks. The content will focus on Earth system science and environmental literacy. The lab experiences will feature a combination of field work, classroom experiments, and computer access to data and visualizations, and demonstrate the rigor and depth of a true lab course. The effort is being funded by NOAA's Environmental Literacy program. One of the prototype units of the course is Investigating Hurricanes. Hurricanes are phenomena which have tremendous impact on humanity and the resources we use. They are also the result of complex interacting Earth systems, making them perfect objects for rigorous investigation of many concepts commonly covered in Earth science courses, such as meteorology, climate, and global wind circulation. Students are able to use the same data sets, analysis tools, and research techniques that scientists employ in their research, yielding truly authentic learning opportunities. This month-long integrated unit uses hurricanes as the story line by

Aircraft Lighting and Transparency Lab

Data.gov (United States)

Federal Laboratory Consortium — The Advanced Lighting and Transparencies with Night Combat Lab performs radiometric and photometric measurements of cockpit lighting and displays. Evaluates the day,...

Creative Science Teaching Labs: New Dimensions in CPD

Science.gov (United States)

Chappell, Kerry; Craft, Anna

2009-01-01

This paper offers analysis and evaluation of "Creative Science Teaching (CST) Labs III", a unique and immersive approach to science teachers' continuing professional development (CPD) designed and run by a London-based organisation, Performing Arts Labs (PAL), involving specialists from the arts, science and technology as integral. Articulating…

Living Labs als een Vehikel voor (Onderwijs)innovatie

NARCIS (Netherlands)

Ellen Sjoer

2014-01-01

Wereldwijd schieten ze als paddenstoelen uit de grond: living labs. Deze ‘levende laboratoria’ zijn er in alle soorten en maten. Meestal wordt het lab gezien als een onderzoeks- en ontwikkelomgeving om een probleem met verschillende partijen op een innovatieve manier op te lossen. De thema’s van de

Fifteen years experience: Egyptian metabolic lab | Fateen | Egyptian ...

African Journals Online (AJOL)

Those patients were classified as: 722 patients (69.4%) with lysosomal storage disorders, 302 patients (29%) with amino acid disorders and 17 patients (1.6%) with galactosemia. Conclusion: This study illustrates the experience of the reference metabolic lab in Egypt over 15 years. The lab began metabolic disorder ...

Panovnické postavy v závěru schodištních maleb

Czech Academy of Sciences Publication Activity Database

Kubínová, Kateřina

2006-01-01

Roč. 13, příl. (2006), s. 23-36 ISSN 1212-1487. [Schodištní cykly Velké věže hradu Karlštejna. Stav po restaurování. Praha, 05.05.2004-07.05.2004] R&D Projects: GA AV ČR(CZ) IAA8033301 Institutional research plan: CEZ:AV0Z80330511 Keywords : Bohemian medieval wall painting * Karlštejn * Charles IV Subject RIV: AL - Art, Architecture, Cultural Heritage

ERLN Technical Support for Labs

Science.gov (United States)

The Environmental Response Laboratory Network provides policies and guidance on lab and data requirements, Standardized Analytical Methods, and technical support for water and radiological sampling and analysis

Respecifying lab ethnography an ethnomethodological study of experimental physics

CERN Document Server

Sormani, Philippe

2014-01-01

Respecifying Lab Ethnography delivers the first ethnomethodological study of current experimental physics in action, describing the disciplinary orientation of lab work and exploring the discipline in its social order, formal stringency and skilful performance - in situ and in vivo. In bringing together two major strands of ethnomethodological inquiry, reflexive ethnography and video analysis, which have hitherto existed in parallel, Respecifying Lab Ethnography introduces a practice-based video analysis. In doing so, the book recasts conventional distinctions to shed fresh light on methodolog

A mobile design lab for user-driven innovation

DEFF Research Database (Denmark)

Christiansen, Ellen; Kanstrup, Anne Marie

2007-01-01

The paper presents the history and conceptual foundation for the Mobile Design Lab, ment to support both designers and users in the acts of user-driven innovation. The Mobile Design Lab is based on Vygotsky's theory of tool- and language-mediation, and was created in 2004 to support research...... and teaching of user driven innovation. Being itself an example of user-driven innovation it has taken shape of HCI design research projekcts, in which we have been involved since 2004. The first challenge was to get 'out of the lab', the next to get 'out of the head', and finally we are currently working...

GeneLab: A Systems Biology Platform for Spaceflight Omics Data

Science.gov (United States)

Reinsch, Sigrid S.; Lai, San-Huei; Chen, Rick; Thompson, Terri; Berrios, Daniel; Fogle, Homer; Marcu, Oana; Timucin, Linda; Chakravarty, Kaushik; Coughlan, Joseph

2015-01-01

NASA's mission includes expanding our understanding of biological systems to improve life on Earth and to enable long-duration human exploration of space. Resources to support large numbers of spaceflight investigations are limited. NASA's GeneLab project is maximizing the science output from these experiments by: (1) developing a unique public bioinformatics database that includes space bioscience relevant "omics" data (genomics, transcriptomics, proteomics, and metabolomics) and experimental metadata; (2) partnering with NASA-funded flight experiments through bio-sample sharing or sample augmentation to expedite omics data input to the GeneLab database; and (3) developing community-driven reference flight experiments. The first database, GeneLab Data System Version 1.0, went online in April 2015. V1.0 contains numerous flight datasets and has search and download capabilities. Version 2.0 will be released in 2016 and will link to analytic tools. In 2015 Genelab partnered with two Biological Research in Canisters experiments (BBRIC-19 and BRIC-20) which examine responses of Arabidopsis thaliana to spaceflight. GeneLab also partnered with Rodent Research-1 (RR1), the maiden flight to test the newly developed rodent habitat. GeneLab developed protocols for maxiumum yield of RNA, DNA and protein from precious RR-1 tissues harvested and preserved during the SpaceX-4 mission, as well as from tissues from mice that were frozen intact during spaceflight and later dissected. GeneLab is establishing partnerships with at least three planned flights for 2016. Organism-specific nationwide Science Definition Teams (SDTs) will define future GeneLab dedicated missions and ensure the broader scientific impact of the GeneLab missions. GeneLab ensures prompt release and open access to all high-throughput omics data from spaceflight and ground-based simulations of microgravity and radiation. Overall, GeneLab will facilitate the generation and query of parallel multi-omics data, and

TN-68 Spent Fuel Transport Cask Analytical Evaluation for Drop Events

International Nuclear Information System (INIS)

Shah, M.J.; Klymyshyn, Nicholas A.; Adkins, Harold E.; Koeppel, Brian J.

2007-01-01

The U.S. Nuclear Regulatory Commission (NRC) is responsible for licensing commercial spent nuclear fuel transported in casks certified by NRC under the Code of Federal Regulations (10 CFR), Title 10, Part 71 (1). Both the International Atomic Energy Agency regulations for transporting radioactive materials (2, paragraph 727), and 10 CFR 71.73 require casks to be evaluated for hypothetical accident conditions, which includes a 9-meter (m) (30-ft) drop-impact event onto a flat, essentially unyielding, horizontal surface, in the most damaging orientation. This paper examines the behavior of one of the NRC certified transportation casks, the TN-68 (3), for drop-impact events. The specific area examined is the behavior of the bolted connections in the cask body and the closure lid, which are significantly loaded during the hypothetical drop-impact event. Analytical work to evaluate the NRC-certified TN-68 spent fuel transport cask (3) for a 9-m (30-ft) drop-impact event on a flat, unyielding, horizontal surface, was performed using the ANSYS (4) and LS DYNA (5) finite-element analysis codes. The models were sufficiently detailed, in the areas of bolt closure interfaces and containment boundaries, to evaluate the structural integrity of the bolted connections under 9-m (30-ft) free-drop hypothetical accident conditions, as specified in 10 CFR 71.73. Evaluation of the cask for puncture, caused by a free drop through a distance of 1-m (40-in.) onto a mild steel bar mounted on a flat, essentially unyielding, horizontal surface, required by 10 CFR 71.73, was not included in the current work, and will have to be addressed in the future. Based on the analyses performed to date, it is concluded that, even though brief separation of the flange and the lid surfaces may occur under some conditions, the seals would close at the end of the drop events, because the materials remain elastic during the duration of the event

Introduction to Computing: Lab Manual. Faculty Guide [and] Student Guide.

Science.gov (United States)

Frasca, Joseph W.

This lab manual is designed to accompany a college course introducing students to computing. The exercises are designed to be completed by the average student in a supervised 2-hour block of time at a computer lab over 15 weeks. The intent of each lab session is to introduce a topic and have the student feel comfortable with the use of the machine…

Green digital signage using nanoparticle embedded narrow-gap field sequential TN-LCDs

Science.gov (United States)

Kobayashi, Shunsuke; Shiraishi, Yukihide; Sawai, Hiroya; Toshima, Naoki; Okita, Masaya; Takeuchi, Kiyofumi; Takatsu, Haruyoshi

2012-03-01

We have fabricated field sequential color (FSC)-LCDs using cells and modules of narrow-gap TN-LCDs with and without doping the nanoparticles of PCyD-ZrO2 and AF-SiO2. It is shown that the FSC-LCD exhibits a high optical efficiency of OE=4.5 that is defined as OE=[Luminance]/[W/m2]=(cd/W). This figure may provide us a good reference or to clear the Energy Star Program Version 5-3 that issues a guideline: LCD with 50 inch on the diagonal consumes the energy of 108W. Through this research it is claimed that our FSC=LCD may be a novel green digital signage.

Digital Social Science Lab

DEFF Research Database (Denmark)

Svendsen, Michael; Lauersen, Christian Ulrich

2015-01-01

At the Faculty Library of Social Sciences (part of Copenhagen University Library) we are currently working intensely towards the establishment of a Digital Social Science Lab (DSSL). The purpose of the lab is to connect research, education and learning processes with the use of digital tools...... at the Faculty of Social Sciences. DSSL will host and facilitate an 80 m2 large mobile and intelligent study- and learning environment with a focus on academic events, teaching and collaboration. Besides the physical settings DSSL has two primary functions: 1. To implement relevant social scientific software...... and hardware at the disposal for students and staff at The Faculty of Social Sciences along with instruction and teaching in the different types of software, e.g. Stata, Nvivo, Atlas.ti, R Studio, Zotero and GIS-software. 2. To facilitate academic events focusing on use of digital tools and analytic software...

Common Systems Integration Lab (CSIL)

Data.gov (United States)

Federal Laboratory Consortium — The Common Systems Integration Lab (CSIL)supports the PMA-209 Air Combat Electronics Program Office. CSIL also supports development, test, integration and life cycle...

Emerging trends in biosensing using stripping voltammetric detection of metal-containing nanolabels – A review

Energy Technology Data Exchange (ETDEWEB)

Kokkinos, Christos; Economou, Anastasios, E-mail: aeconomo@chem.uoa.gr

2017-04-08

Over the last years, nanomaterials have found many applications in the development of electrochemical biosensors. Among other functions, metal nanoparticles (NPs) and quantum dots (QDs) (semiconducting nanocrystals composed of metal salts) are increasingly being used as voltammetric labels in affinity biosensing. Labeling is based on the attachment of the label(s) on the target biomolecules or on a biorecognition reporting probe. After an appropriate specific affinity interaction between the target and the reporting probe, the metallic nanolabels are converted to the respective cations which are quantified by a voltammetric technique. The very use of metal-containing nanoprobes as labels provides a first amplification step since each nanoprobe can release a very significant number of detectable cations. When anodic stripping voltammetry (ASV) (in which a preconcentration step precedes the actual voltammetric scan) is further employed as the detection format, ultra-sensitive bioassays can be developed. The present paper reviews the emerging trends in affinity biosensing using ASV detection of metal-containing nanolabels. It provides a critical discussion of recent developments in ASV transduction and electrodes, novel strategies for signal enhancement, approaches for multiplexed detection as well as fluidics, paper-based and lab-on-a-chip devices. - Highlights: • This paper reviews the use of ASV for affinity biosensing with metal-containing nanolabels. • Both metal nanoparticles and quantum dots applications are considered. • Transducers and new electrode materials are covered. • Signal enhancement and multiplexing strategies are discussed. • Sensor arrays, paper-based, fluidic and lab-on-chip applications are described.

Numerical studies on a plasmonic temperature nanosensor based on a metal-insulator-metal ring resonator structure for optical integrated circuit applications

Science.gov (United States)

Al-mahmod, Md. Jubayer; Hyder, Rakib; Islam, Md Zahurul

2017-07-01

A nanosensor, based on a metal-insulator-metal (MIM) plasmonic ring resonator, is proposed for potential on-chip temperature sensing and its performance is evaluated numerically. The sensor components can be fabricated by using planar processes on a silicon substrate, making its manufacturing compatible to planar electronic fabrication technology. The sensor, constructed using silver as the metal rings and a thermo-optic liquid ethanol film between the metal layers, is capable of sensing temperature with outstanding optical sensitivity, as high as -0.53 nm/°C. The resonance wavelength is found to be highly sensitive to the refractive index of the liquid dielectric film. The resonance peak can be tuned according to the requirement of intended application by changing the radii of the ring resonator geometries in the design phase. The compact size, planar and silicon-based design, and very high resolutions- these characteristics are expected to make this sensor technology a preferred choice for lab-on-a-chip applications, as compared to other contemporary sensors.

Timing properties and pulse shape discrimination of LAB-based liquid scintillator

International Nuclear Information System (INIS)

Li Xiaobo; Xiao Hualin; Cao Jun; Li Jin; Heng Yuekun; Ruan Xichao

2011-01-01

Linear Alkyl Benzene (LAB) is a promising liquid scintillator solvent in neutrino experiments because it has many appealing properties. The timing properties of LAB-based liquid scintillator have been studied through ultraviolet and ionization excitation in this study. The decay time of LAB, PPO and bis-MSB is found to be 48.6 ns, 1.55 ns and 1.5 ns, respectively. A model can describe the absorption and re-emission process between PPO and bis-MSB perfectly. The energy transfer time between LAB and PPO with different concentrations can be obtained via another model. We also show that the LAB-based liquid scintillator has good (n, γ) and (α, γ) discrimination power. (authors)

A Moodle extension to book online labs

Directory of Open Access Journals (Sweden)

Antonio C. Cardoso

2005-11-01

Full Text Available The social constructivist philosophy of Moodle makes it an excellent choice to deliver e-learning contents that require collaborative activities, such as those that are associated with online labs. In the case of online labs that enable web access to real devices (remote workbenches, access time should be reserved beforehand. A booking tool will avoid access conflicts and at the same time will help the students to organise their time and activities. This paper presents a Moodle extension that was developed within the Leonardo da Vinci MARVEL project, with the objective of meeting this requirement. The booking tool presented enables resource sharing in general and may be used to organise access to any type of scarce resources, such as to online labs and to the videoconferencing rooms that are needed to support collaborative activities.

'Test n Treat (TnT)': a cluster-randomised feasibility trial of frequent, rapid-testing and same-day, on-site treatment to reduce rates of chlamydia in high-risk further education college students: statistical analysis plan.

Science.gov (United States)

Phillips, Rachel; Oakeshott, Pippa; Kerry-Barnard, Sarah; Reid, Fiona

2018-06-05

There are high rates of sexually transmitted infections (STIs) in ethnically diverse, sexually active students aged 16-24 years attending London further education (FE) colleges. However, uptake of chlamydia screening remains low. The TnT study aims to assess the feasibility of conducting a future trial in FE colleges to investigate if frequent, rapid, on-site testing and treatment (TnT) reduces chlamydia rates. This article presents the statistical analysis plan for the main study publication as approved and signed off by the Trial Management Group prior to the first data extraction for the final report. TnT is a cluster-randomised feasibility trial conducted over 7 months with parallel qualitative and economic assessments. Colleges will be randomly allocated into the intervention (TnT) or the control group (no TnT). Six FE colleges in London will be included. At each college for 2 days, 80 consecutive sexually active students aged 16-24 years (total 480 students across all six colleges) will be recruited from public areas and asked to provide baseline samples. One and 4 months after recruitment intervention colleges will be visited on two consecutive days by the TnT team where participating students will be texted and invited to come for same-day, on-site, rapid chlamydia testing and, if positive, treatment. Participants in the control colleges will receive 'thank you' texts 1 and 4 months after recruitment. Seven months after recruitment, participants from both groups will be invited to complete questionnaires and provide samples for TnT. All samples will be tested, and same-day treatment offered to participants with positive results. Key feasibility outcomes include: recruitment rates, testing and treatment uptake rates (at 1 and 4 months) and follow-up rates (at 7 months). ISRCTN 58038795 . Registered on 31 August 2016.

Advanced Active Acoustics Lab (AAAL)

Data.gov (United States)

Federal Laboratory Consortium — The Advanced Active Acoustics Lab (AAAL) is a state-of-the-art Undersea Warfare (USW) acoustic data analysis facility capable of both active and passive underwater...

Towards Third-Generation Living Lab Networks in Cities

Directory of Open Access Journals (Sweden)

Seppo Leminen

2017-11-01

Full Text Available Many cities engage in diverse experimentation, innovation, and development activities with a broad variety of environments and stakeholders to the benefit of citizens, companies, municipalities, and other organizations. Hence, this article discusses such engagement in terms of next-generation living lab networks in the city context. In so doing, the study contributes to the discussion on living labs by introducing a framework of collaborative innovation networks in cities and suggesting a typology of third-generation living labs. Our framework is characterized by diverse platforms and participation approaches, resulting in four distinctive modes of collaborative innovation networks where the city is: i a provider, ii a neighbourhood participator, iii a catalyst, or iv a rapid experimenter. The typology is based on an analysis of 118 interviews with participants in six Finnish cities and reveals various ways to organize innovation activities in the city context. In particular, cities can benefit from innovation networks by simultaneously exploiting multiple platforms such as living labs for innovation. We conclude by discussing implications to theory and practice, and suggesting directions for future research.

Reaction of LaB6 with MoSi2

International Nuclear Information System (INIS)

Ordan'yan, S.S.; Gardagina, E.N.; Barabanova, S.N.

1989-01-01

Investigation results of interaction in LaB 6 -MoSi 2 system within wide concentration and temperature ranges are presented. LaB 6 and MoSi 2 powders were preliminary squeezed out in vacuum at 1470 K. Presence of LaB 6 and MoSi 2 initial phases only is determined using X-ray pahse analysis of sintered and melted specimens of all compositions. Traces of MoB (probably, due to quick crystallization after melting and partial evaporation of silicon from the melt) are detected in some alloys exposed to melting

Study Labs Kortlægningsrapport UCSJ

DEFF Research Database (Denmark)

Jørnø, Rasmus Leth Vergmann; Hestbech, Astrid Margrethe; Gynther, Karsten

2015-01-01

Rapporten er en delleverance i det regionale forprojekt S​tudy Labs,​der udføres som et samarbejde mellem Holbæk, Odsherred og Kalundborg kommune og University College Sjælland (UCSJ). Samarbejdet er delvist medfinansieret af Region Sjælland. Rapporten behandler projektets etableringsfase...... for at nå de kommunale målsætninger. De potentielle målgrupper er blevet kortlagt. Samtidig er undersøgelser i brugergrupperne blevet gjort håndgribelige i form af Personaer. Kommunerne har, faciliteret af Educationlab, gennemført designworkshops og er fremkommet med designs for Study Labs, der som...

What Is LAB and Why Was It Renormed?

Science.gov (United States)

Abbott, Muriel

A report on the Language Assessment Battery (LAB) explains, in question-and-answer form, the causes and results of some changes made in the test norms. The LAB is a test of communicative language competence, written in English and Spanish versions and used for student placement in the New York City Public Schools. The report describes the test…

FOREWORD: Jefferson Lab: A Long Decade of Physics

Science.gov (United States)

Montgomery, Hugh

2011-04-01

Jefferson Lab Jefferson Lab was created in 1984 and started operating in about 1996. 2011 is an appropriate time to try to take a look at the results that have appeared, what has been learned, and what has been exciting for our scientific community. Rather than attempt to construct a coherent view with a single author or at least a small number, we have, instead, invited small groups of people who have been intimately involved in the work itself to make contributions. These people are accelerator experts, experimentalists and theorists, staff and users. We have, in the main, sought reviews of the actual sub-fields. The primary exception is the first paper, which sets the scene as it was, in one person's view, at the beginning of Jefferson Lab. In reviewing the material as it appeared, I was impressed by the breadth of the material. Major advances are documented from form factors to structure functions, from spectroscopy to physics beyond the standard model of nuclear and particle physics. Recognition of the part played by spin, the helicities of the beams, the polarizations of the targets, and the polarizations of final state particles, is inescapable. Access to the weak interaction amplitudes through measurements of the parity violating asymmetries has led to quantification of the strange content of the nucleon and the neutron radius of lead, and to measurements of the electroweak mixing angle. Lattice QCD calculations flourished and are setting the platform for understanding of the spectroscopy of baryons and mesons. But the star of the game was the accelerator. Its performance enabled the physics and also the use of the technology to generate a powerful free electron laser. These important pieces of Jefferson Lab physics are given their place. As the third Director of Jefferson Lab, and on behalf of the other physicists and others presently associated with the lab, I would like to express my admiration and gratitude for the efforts of the directors, chief

Jefferson Lab: A Long Decade of Physics

International Nuclear Information System (INIS)

Montgomery, Hugh

2011-01-01

Jefferson Lab was created in 1984 and started operating in about 1996. 2011 is an appropriate time to try to take a look at the results that have appeared, what has been learned, and what has been exciting for our scientific community. Rather than attempt to construct a coherent view with a single author or at least a small number, we have, instead, invited small groups of people who have been intimately involved in the work itself to make contributions. These people are accelerator experts, experimentalists and theorists, staff and users. We have, in the main, sought reviews of the actual sub-fields. The primary exception is the first paper, which sets the scene as it was, in one person's view, at the beginning of Jefferson Lab. In reviewing the material as it appeared, I was impressed by the breadth of the material. Major advances are documented from form factors to structure functions, from spectroscopy to physics beyond the standard model of nuclear and particle physics. Recognition of the part played by spin, the helicities of the beams, the polarizations of the targets, and the polarizations of final state particles, is inescapable. Access to the weak interaction amplitudes through measurements of the parity violating asymmetries has led to quantification of the strange content of the nucleon and the neutron radius of lead, and to measurements of the electroweak mixing angle. Lattice QCD calculations flourished and are setting the platform for understanding of the spectroscopy of baryons and mesons. But the star of the game was the accelerator. Its performance enabled the physics and also the use of the technology to generate a powerful free electron laser. These important pieces of Jefferson Lab physics are given their place. As the third Director of Jefferson Lab, and on behalf of the other physicists and others presently associated with the lab, I would like to express my admiration and gratitude for the efforts of the directors, chief scientists

Undergraduate Student Construction and Interpretation of Graphs in Physics Lab Activities

Science.gov (United States)

Nixon, Ryan S.; Godfrey, T. J.; Mayhew, Nicholas T.; Wiegert, Craig C.

2016-01-01

Lab activities are an important element of an undergraduate physics course. In these lab activities, students construct and interpret graphs in order to connect the procedures of the lab with an understanding of the related physics concepts. This study investigated undergraduate students' construction and interpretation of graphs with best-fit…

Outreach Science Education: Evidence-Based Studies in a Gene Technology Lab

Science.gov (United States)

Scharfenberg, Franz-Josef; Bogner, Franz X.

2014-01-01

Nowadays, outreach labs are important informal learning environments in science education. After summarizing research to goals outreach labs focus on, we describe our evidence-based gene technology lab as a model of a research-driven outreach program. Evaluation-based optimizations of hands-on teaching based on cognitive load theory (additional…

77 FR 59573 - Proposed Amendment of Class D and E Airspace; Tri-Cities, TN; Revocation of Class E Airspace; Tri...

Science.gov (United States)

2012-09-28

... at Hawkins County Airport, Rogersville, TN, and Virginia Highlands Airport, Abington, VA. The Tri... separation of existing Class E airspace surrounding Virginia Highlands Airport, Abingdon, VA, and Hawkins... publication, the FAA reassessed the proposal to show the separation of Hawkins County Airport, and Virginia...

Virtual Labs in proteomics: new E-learning tools.

Science.gov (United States)

Ray, Sandipan; Koshy, Nicole Rachel; Reddy, Panga Jaipal; Srivastava, Sanjeeva

2012-05-17

Web-based educational resources have gained enormous popularity recently and are increasingly becoming a part of modern educational systems. Virtual Labs are E-learning platforms where learners can gain the experience of practical experimentation without any direct physical involvement on real bench work. They use computerized simulations, models, videos, animations and other instructional technologies to create interactive content. Proteomics being one of the most rapidly growing fields of the biological sciences is now an important part of college and university curriculums. Consequently, many E-learning programs have started incorporating the theoretical and practical aspects of different proteomic techniques as an element of their course work in the form of Video Lectures and Virtual Labs. To this end, recently we have developed a Virtual Proteomics Lab at the Indian Institute of Technology Bombay, which demonstrates different proteomics techniques, including basic and advanced gel and MS-based protein separation and identification techniques, bioinformatics tools and molecular docking methods, and their applications in different biological samples. This Tutorial will discuss the prominent Virtual Labs featuring proteomics content, including the Virtual Proteomics Lab of IIT-Bombay, and E-resources available for proteomics study that are striving to make proteomic techniques and concepts available and accessible to the student and research community. This Tutorial is part of the International Proteomics Tutorial Programme (IPTP 14). Details can be found at: http://www.proteomicstutorials.org/. Copyright © 2012 Elsevier B.V. All rights reserved.

Some Case Studies on Metal-Microbe Interactions to Remediate Heavy Metals- Contaminated Soils in Korea

Science.gov (United States)

Chon, Hyo-Taek

2015-04-01

Conventional physicochemical technologies to remediate heavy metals-contaminated soil have many problems such as low efficiency, high cost and occurrence of byproducts. Recently bioremediation technology is getting more and more attention. Bioremediation is defined as the use of biological methods to remediate and/or restore the contaminated land. The objectives of bioremediation are to degrade hazardous organic contaminants and to convert hazardous inorganic contaminants to less toxic compounds of safe levels. The use of bioremediation in the treatment of heavy metals in soils is a relatively new concept. Bioremediation using microbes has been developed to remove toxic heavy metals from contaminated soils in laboratory scale to the contaminated field sites. Recently the application of cost-effective and environment-friendly bioremediation technology to the heavy metals-contaminated sites has been gradually realized in Korea. The merits of bioremediation include low cost, natural process, minimal exposure to the contaminants, and minimum amount of equipment. The limitations of bioremediation are length of remediation, long monitoring time, and, sometimes, toxicity of byproducts for especially organic contaminants. From now on, it is necessary to prove applicability of the technologies to contaminated sites and to establish highly effective, low-cost and easy bioremediation technology. Four categories of metal-microbe interactions are generally biosorption, bioreduction, biomineralization and bioleaching. In this paper, some case studies of the above metal-microbe interactions in author's lab which were published recently in domestic and international journals will be introduced and summarized.

Development and Interaction between LMS Services and Remote Labs

Directory of Open Access Journals (Sweden)

Manuel Castro

2008-03-01

Full Text Available Nowadays there is a great number of universities and organizations working in e-learning and i-learning solutions. One of the most well-known is the learning management system or LMS that allows displaying theoretical content in an organized and controlled way. In some jobs and studies it is necessary for the student to get a practical knowledge as well as a theoretical one. To obtain this practical knowledge, the universities and organizations are developing Virtual, Remote and Web labs. At these moments the LMS and Web labs are working independently. We are studying a new architecture allowing the integration of the LMS with different Web labs. This architecture must allow the students, teachers and administrators to use the services of LMS and virtual lab’s features as if they were working with the same software.

Living lab: Format for rehearsing a new (service) practice

DEFF Research Database (Denmark)

Yndigegn, Signe; Aakjær, Marie Kirstejn

Citizen engagement and the citizens as a resource are key concepts in rethinking the Danish welfare system to meet the challenges of delivering better services for the elderly, while simultaneously reducing the cost of healthcare. In this method paper, we address how the co-design of new digital...... service platforms takes place in the format of living labs. We characterize living labs as the design of experiential spaces where ‘what is’ and ‘what could be’ are explored over a longer period of engagement. The labs are staged to integrate multiple stakeholders’ issues and resources and to create new...... technologies, concepts, or service designs. This paper unpacks the practices of living labs with questions of what is being produced, not only in terms of products, but also in terms of changes in practices, roles, and relations. To analyze and discuss this question the authors report about their engagement...

Acute Inhalation Exposure to Titanium Ethanolate as a Possible Cause of Metal Fume Fever

Directory of Open Access Journals (Sweden)

M Ahmadimanesh

2014-04-01

Full Text Available Occupational inhalation exposure to noxious agents is not uncommon. Herein, we present a 26-year-old male student who had accidental acute inhalation exposure to a large quantity of titanium ethanolate and hydrogen chloride in chemistry lab. He was referred to the emergency department of our hospital with low-grade fever, dyspnea, headache, fatigue and myalgia. After 24 hrs of symptomatic treatment (oxygen therapy and acetaminophen, the fever was subsided and the patient discharged home in a good clinical condition. The presented symptoms could be interpreted as a form of metal fume fever. It can therefore be concluded that organo-metallic compound of titanium metal may have the potential to produce metal fume fever in human.

CELSTEC Learning Labs: Mobile App Development for Education and Training

NARCIS (Netherlands)

Specht, Marcus

2011-01-01

Specht, M. (2011). CELSTEC Learning Labs: Mobile App Development for Education and Training. Presentation given in Workshop at CELSTEC Learning Lab for Bluetea. February, 21, 2011, Heerlen, The Netherlands.

Pollution hazard closes neutrino lab

CERN Multimedia

Jones, Nicola

2003-01-01

"A leading astrophysics laboratory in Italy has closed down all but one of its experiments over concerns that toxic polluants could leak form the underground lab into the local water supply" (0.5 page)

Living Labs as boundary-spanners between Triple Helix actors

NARCIS (Netherlands)

van Geenhuizen, M.S.

2016-01-01

Living labs are an increasingly popular methodology to enhance innovation. Living labs aim to span boundaries between different organizations, among others Triple helix actors, by acting as a network organization typically in a real-life environment to foster co-creation by user-groups. This paper

Characterization of Tn3000, a Transposon Responsible for blaNDM-1 Dissemination among Enterobacteriaceae in Brazil, Nepal, Morocco, and India.

Science.gov (United States)

Campos, Juliana Coutinho; da Silva, Maria José Félix; dos Santos, Paulo Roberto Nascimento; Barros, Elaine Menezes; Pereira, Mayne de Oliveira; Seco, Bruna Mara Silva; Magagnin, Cibele Massotti; Leiroz, Leonardo Kalab; de Oliveira, Théo Gremen Mimary; de Faria-Júnior, Célio; Cerdeira, Louise Teixeira; Barth, Afonso Luís; Sampaio, Suely Carlos Ferreira; Zavascki, Alexandre Prehn; Poirel, Laurent; Sampaio, Jorge Luiz Mello

2015-12-01

In Enterobacteriaceae, the blaNDM genes have been found in many different genetic contexts, and a wide diversity of plasmid scaffolds bearing those genes has been found. In August 2013, we identified NDM-1-producing Escherichia coli and Enterobacter hormaechei strains from a single rectal swab sample from a patient hospitalized in Rio de Janeiro, Brazil, who had no history of travel abroad. Complete DNA sequencing using the Illumina platform and annotation of the two plasmids harboring the blaNDM-1 gene, one from each strain, showed that they belonged to incompatibility groups IncFIIK and IncX3 and harbored a novel transposon named Tn3000. Similar genetic structures have been identified among other isolates in Brazil but also on plasmids from other continents. Our findings suggest that the blaNDM-1 gene may be transmitted by Tn3000 in different parts of the world. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

An Annotated Math Lab Inventory.

Science.gov (United States)

Schussheim, Joan Yares

1980-01-01

A listing of mathematics laboratory material is organized as follows: learning kits, tape programs, manipulative learning materials, publications, math games, math lab library, and an alphabetized listing of publishers and/or companies offering materials. (MP)

Connect high speed analog-digital converter with EPICS based on LabVIEW

International Nuclear Information System (INIS)

Wang Wei; Chi Yunlong

2008-01-01

This paper introduce a method to connect high speed analog-digital converter (ADC212/100) with EPICS on Windows platform using LabVIEW. We use labVIEW to communicate with the converter, then use interface sub-VIs between LabVIEW and EPICS to access the EPICS IOC by Channel Access (CA). For the easy use graph programming language of LabVIEW, this method could shorten the develop period and reduce manpower cost. (authors)

Electronics lab instructors' approaches to troubleshooting instruction

Science.gov (United States)

Dounas-Frazer, Dimitri R.; Lewandowski, H. J.

2017-06-01

In this exploratory qualitative study, we describe instructors' self-reported practices for teaching and assessing students' ability to troubleshoot in electronics lab courses. We collected audio data from interviews with 20 electronics instructors from 18 institutions that varied by size, selectivity, and other factors. In addition to describing participants' instructional practices, we characterize their perceptions about the role of troubleshooting in electronics, the importance of the ability to troubleshoot more generally, and what it means for students to be competent troubleshooters. One major finding of this work is that, while almost all instructors in our study said that troubleshooting is an important learning outcome for students in electronics lab courses, only half of instructors said they directly assessed students' ability to troubleshoot. Based on our findings, we argue that there is a need for research-based instructional materials that attend to both cognitive and noncognitive aspects of troubleshooting proficiency. We also identify several areas for future investigation related to troubleshooting instruction in electronics lab courses.

Heavy metals in surface sediments of the Jialu River, China: Their relations to environmental factors

Energy Technology Data Exchange (ETDEWEB)

Fu, Jie [State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093 (China); Environmental Engineering Program, Department of Civil Engineering, Auburn University, Auburn, AL 36849 (United States); Zhao, Changpo [State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093 (China); Luo, Yupeng [Department of Mathematics and Statistics, Auburn University, Auburn, AL 36849 (United States); Liu, Chunsheng, E-mail: liuchunshengidid@126.com [College of Fisheries, Huazhong Agricultural University, Wuhan 430070 (China); Kyzas, George Z. [Laboratory of General and Inorganic Chemical Technology, Department of Chemistry, Aristotle University of Thessaloniki, Thessaloniki 54124 (Greece); Luo, Yin [State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093 (China); Zhao, Dongye [Environmental Engineering Program, Department of Civil Engineering, Auburn University, Auburn, AL 36849 (United States); An, Shuqing [State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093 (China); Zhu, Hailiang, E-mail: zhuhl@nju.edu.cn [State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093 (China)

2014-04-01

Highlights: • Zhengzhou City had major effect on the pollution of the Jialu River. • TN, OP, TP and COD{sub Mn} in water drove heavy metals to deposit in sediments. • B-IBI was sensitive to the adverse effect of heavy metals in sediments. - Abstract: This work investigated heavy metal pollution in surface sediments of the Jialu River, China. Sediment samples were collected at 19 sites along the river in connection with field surveys and the total concentrations were determined using atomic fluorescence spectrometer and inductively coupled plasma optical emission spectrometer. Sediment samples with higher metal concentrations were collected from the upper reach of the river, while sediments in the middle and lower reaches had relatively lower metal concentrations. Multivariate techniques including Pearson correlation, hierarchical cluster and principal components analysis were used to evaluate the metal sources. The ecological risk associated with the heavy metals in sediments was rated as moderate based on the assessments using methods of consensus-based Sediment Quality Guidelines, Potential Ecological Risk Index and Geo-accumulation Index. The relations between heavy metals and various environmental factors (i.e., chemical properties of sediments, water quality indices and aquatic organism indices) were also studied. Nitrate nitrogen, total nitrogen, and total polycyclic aromatic hydrocarbons concentrations in sediments showed a co-release behavior with heavy metals. Ammonia nitrogen, total nitrogen, orthophosphate, total phosphate and permanganate index in water were found to be related to metal sedimentation. Heavy metals in sediments posed a potential impact on the benthos community.

Heavy metals in surface sediments of the Jialu River, China: Their relations to environmental factors

International Nuclear Information System (INIS)

Fu, Jie; Zhao, Changpo; Luo, Yupeng; Liu, Chunsheng; Kyzas, George Z.; Luo, Yin; Zhao, Dongye; An, Shuqing; Zhu, Hailiang

2014-01-01

Highlights: • Zhengzhou City had major effect on the pollution of the Jialu River. • TN, OP, TP and COD Mn in water drove heavy metals to deposit in sediments. • B-IBI was sensitive to the adverse effect of heavy metals in sediments. - Abstract: This work investigated heavy metal pollution in surface sediments of the Jialu River, China. Sediment samples were collected at 19 sites along the river in connection with field surveys and the total concentrations were determined using atomic fluorescence spectrometer and inductively coupled plasma optical emission spectrometer. Sediment samples with higher metal concentrations were collected from the upper reach of the river, while sediments in the middle and lower reaches had relatively lower metal concentrations. Multivariate techniques including Pearson correlation, hierarchical cluster and principal components analysis were used to evaluate the metal sources. The ecological risk associated with the heavy metals in sediments was rated as moderate based on the assessments using methods of consensus-based Sediment Quality Guidelines, Potential Ecological Risk Index and Geo-accumulation Index. The relations between heavy metals and various environmental factors (i.e., chemical properties of sediments, water quality indices and aquatic organism indices) were also studied. Nitrate nitrogen, total nitrogen, and total polycyclic aromatic hydrocarbons concentrations in sediments showed a co-release behavior with heavy metals. Ammonia nitrogen, total nitrogen, orthophosphate, total phosphate and permanganate index in water were found to be related to metal sedimentation. Heavy metals in sediments posed a potential impact on the benthos community

The Influence of Tablet PCs on Students' Use of Multiple Representations in Lab Reports

Science.gov (United States)

Guelman, Clarisa Bercovich; De Leone, Charles; Price, Edward

2009-11-01

This study examined how different tools influenced students' use of representations in the Physics laboratory. In one section of a lab course, every student had a Tablet PC that served as a digital-ink based lab notebook. Students could seamlessly create hand-drawn graphics and equations, and write lab reports on the same computer used for data acquisition, simulation, and analysis. In another lab section, students used traditional printed lab guides, kept paper notebooks, and then wrote lab reports on regular laptops. Analysis of the lab reports showed differences between the sections' use of multiple representations, including an increased use of diagrams and equations by the Tablet users.

FameLab Switzerland: a CERN PhD student triumphs

CERN Multimedia

Alexander Brown

2013-01-01

Would you be able to explain your work to a non-specialist in just three minutes? On Friday 24 May, the Swiss national final of FameLab saw six young researchers from CERN attempt just that. FameLab is an international competition in the style of a TV talent show, seeking out the next generation of talent in science communication.   Participants in the Swiss national final of FameLab alongside Deni Subasic, presenter of the event (far left), on Friday 24 May. Having qualified from the Geneva heat held in the Globe in March, the six CERN representatives took to the stage in Moods bar in Zurich. As well as particle physics, from the fundamental building blocks (literally) of the Standard Model to medical applications, the line-up featured immunology, neurology and genetics. Although slideshows are strictly banned from FameLab, other visual props are strongly encouraged. For instance, Piotr Traczyk (CMS) represented the apparent chaos of particle collisions by throwing together two decks of ca...

Interfacing LabVIEW With Instrumentation for Electronic Failure Analysis and Beyond

Science.gov (United States)

Buchanan, Randy K.; Bryan, Coleman; Ludwig, Larry

1996-01-01

The Laboratory Virtual Instrumentation Engineering Workstation (LabVIEW) software is designed such that equipment and processes related to control systems can be operationally lined and controlled by the use of a computer. Various processes within the failure analysis laboratories of NASA's Kennedy Space Center (KSC) demonstrate the need for modernization and, in some cases, automation, using LabVIEW. An examination of procedures and practices with the Failure Analaysis Laboratory resulted in the conclusion that some device was necessary to elevate the potential users of LabVIEW to an operational level in minimum time. This paper outlines the process involved in creating a tutorial application to enable personnel to apply LabVIEW to their specific projects. Suggestions for furthering the extent to which LabVIEW is used are provided in the areas of data acquisition and process control.

Characterization of Lactic Acid Bacteria (LAB) isolated from Indonesian shrimp paste (terasi)

Science.gov (United States)

Amalia, U.; Sumardianto; Agustini, T. W.

2018-02-01

Shrimp paste was one of fermented products, popular as a taste enhancer in many dishes. The processing of shrimp paste was natural fermentation, depends on shrimp it self and the presence of salt. The salt inhibits the growth of undesirable microorganism and allows the salt-tolerant lactic acid bacteria (LAB) to ferment the protein source to lactic acids. The objectives of this study were to characterize LAB isolated from Indonesian shrimp paste or "Terasi" with different times of fermentation (30, 60 and 90 days). Vitech analysis showed that there were four strains of the microorganism referred to as lactic acid bacteria (named: LABS1, LABS2, LABS3 and LABS4) with 95% sequence similarity. On the basis of biochemical, four isolates represented Lactobacillus, which the name Lactobacillus plantarum is proposed. L.plantarum was play role in resulting secondary metabolites, which gave umami flavor in shrimp paste.

A Remote Direct Sequence Spread Spectrum Communications Lab Utilising the Emona DATEx

Directory of Open Access Journals (Sweden)

Cosmas Mwikirize

2012-12-01

Full Text Available Remote labs have become popular learning aids due to their versatility and considerable ease of utilisation as compared to their physical counterparts. At Makerere University, the remote labs are based on the standard Massachusetts Institute of Technology (MIT iLabs Shared Architecture (ISA - a scalable and generic platform. Presented in this paper is such a lab, addressing the key practical aspects of Direct Sequence Spread Spectrum (DSSS communication. The lab is built on the National Instruments Educational Laboratory Virtual Instrumentation Suite (NI ELVIS with the Emona Digital and Analog Telecommunications Experimenter (DATEx add-on board. It also incorporates switching hardware. The lab facilitates real-time control of the equipment, with users able to set, manipulate and observe signal parameters in both the frequency and the time domains. Simulation and data Acquisition modes of the experiment are supported to provide a richer learning experience.

A large inversion in the linear chromosome of Streptomyces griseus caused by replicative transposition of a new Tn3 family transposon.

Science.gov (United States)

Murata, M; Uchida, T; Yang, Y; Lezhava, A; Kinashi, H

2011-04-01

We have comprehensively analyzed the linear chromosomes of Streptomyces griseus mutants constructed and kept in our laboratory. During this study, macrorestriction analysis of AseI and DraI fragments of mutant 402-2 suggested a large chromosomal inversion. The junctions of chromosomal inversion were cloned and sequenced and compared with the corresponding target sequences in the parent strain 2247. Consequently, a transposon-involved mechanism was revealed. Namely, a transposon originally located at the left target site was replicatively transposed to the right target site in an inverted direction, which generated a second copy and at the same time caused a 2.5-Mb chromosomal inversion. The involved transposon named TnSGR was grouped into a new subfamily of the resolvase-encoding Tn3 family transposons based on its gene organization. At the end, terminal diversity of S. griseus chromosomes is discussed by comparing the sequences of strains 2247 and IFO13350.

Innovative Use of a Classroom Response System During Physics Lab

Science.gov (United States)

Walgren, Jay

2011-01-01

More and more physics instructors are making use of personal/classroom response systems or "clickers." The use of clickers to engage students with multiple-choice questions during lecture and available instructor resources for clickers have been well documented in this journal.1-4 Newer-generation clickers, which I refer to as classroom response systems (CRS), have evolved to accept numeric answers (such as 9.81) instead of just single "multiple-choice" entries (Fig. 1). This advancement is available from most major clicker companies and allows for a greater variety of engaging questions during lecture. In addition, these new "numeric ready" clickers are marketed to be used for student assessments. During a test or quiz, students' answers are entered into their clicker instead of on paper or Scantron® and immediately absorbed by wireless connection into a computer for grading and analysis. I recognize the usefulness and benefit these new-generation CRSs provide for many instructors. However, I do not use my CRS in either of the aforementioned activities. Instead, I use it in an unconventional way. I use the CRS to electronically capture students' lab data as they are performing a physics lab (Fig. 2). I set up the clickers as if I were going to use them for a test, but instead of entering answers to a test, my students enter lab data as they collect it. In this paper I discuss my use of a classroom response system during physics laboratory and three benefits that result: 1) Students are encouraged to "take ownership of" and "have integrity with" their physics lab data. 2) Students' measuring and unit conversion deficiencies are identified immediately during the lab. 3) The process of grading students' labs is simplified because the results of each student's lab calculations can be pre-calculated for the instructor using a spreadsheet. My use of clickers during lab can be implemented with most clicker systems available to instructors today. The CRS I use is the e

First results on GlioLab/GlioSat Precursors Missions

Science.gov (United States)

Cappelletti, Chantal; Notarangelo, Angelo; Demoss, Darrin; Carella, Massimo

2012-07-01

Since 2009 GAUSS group is involved in a joint collaboration with Morehead State University (MSU) Space Science Center and IRCCS Casa Sollievo della Sofferenza (CSS) research labs with the aim to design a biomedical project in order to investigate if the combined effects of microgravity conditions and ionizing radiation increase or decrease the survival rate of cancer cells. The biological sample consists of Glioblastoma cancer cell line ANGM-CSS. Glioblastoma is a kind of cancer that can be treated after surgery only by radiotherapy using ionizing radiation. This treatment, anyway, results in a very low survival rate. This project uses different university space platforms: a CubeLab, named GlioLab, on board the International Space Station and the university microsatellite UniSat-5 designed by GAUSS. In addition a GlioLab/GlioSat precursor experiment has already flown two times with the Space Shuttle during the missions STS-134 and STS-135. The phase 0 or the precursor of GlioLab uses a COTS system, named Liquid Mixing Apparatus (LMA), to board the biological samples inside the Space Shuttle for thirty day . The LMA allows to board liquids inside a vial but is not equipped with environment control system. After landing the samples were investigated by researchers at CSS in Italy and at MSU in Kentucky. This paper deals with the experimental set up and the results obtained during the STS-134 and STS-135 missions and with the new evidences on the behavior of this kind of cancer. In particular the results obtained on the DNA analysis give a confirmation of the original idea of GLioLab/Gliosat project justifying the development of the two systems.

Secondary side TSP deposit buildup: lab test investigation focused on electrokinetic considerations

International Nuclear Information System (INIS)

Barale, M.; Guillodo, M.; Foucault, M.; Ryckelynck, N.; Clinard, M-H.; Chahma, F.; Brun, C.; Corredera, G.

2010-01-01

Deposit buildup which caused the clogging of the 'foils' of the upper tube-support-plates (TSP) inside a PWR steam generator of French NPPs in 2006 presents certain similarities with deposits observed in lab tests performed in secondary coolant chemistry at the Technical Centre of AREVA NP in 2002. The mechanism of TSP clogging seems not to present obvious phenomenological links with the fouling of the free span of SG since deposits buildup is quite uniform and is currently related to a surface boiling effect due to the surface heat flux. A specific mechanism could account for TSP clogging. In particular, electrokinetic effects were investigated by EDF-CEIDRE and AREVA NP SAS in the framework of a lab test program started in 2007. The electrokinetic approach is to consider that the coupling of local hydrodynamic and surface electrochemistry could lead to the formation of a very localized and heterogeneous deposit at the leading edge between both TSP and SG tubing material. Electrokinetic effects can lead to the oxidation and/or the precipitation of ferrous ions and to a variation of the electrokinetic potential which can produce strong attraction of iron oxide colloids. These electrokinetic effects are dependent of the T/H and local hydrodynamic conditions and surface electrochemistry explaining. The objective of this EDF-AREVA lab test program is to investigate the role of secondary chemistry coolant (pH, DH, N 2 H 4 , amine, redox) and of the nature of materials (SS, Ni base alloy) on deposit buildup. Properties of oxide surface and zeta potential of oxidized metallic materials have been also determined at temperature to understand their potential contribution on mechanism of TSP clogging in secondary side chemistry coolant. In this paper, a set of specific experiments carried out in this frame have been presented and discussed, paying particular attention to the effects of electrokinetic considerations and surface charges at oxide-solution interfaces

Secondary side TSP deposit buildup: lab test investigation focused on electrokinetic considerations

Energy Technology Data Exchange (ETDEWEB)

Barale, M.; Guillodo, M.; Foucault, M., E-mail: Morgan.Barale@areva.com [AREVA NP SAS, Technical Centre, Le Creusot (France); Ryckelynck, N.; Clinard, M-H.; Chahma, F.; Brun, C. [AREVA NP SAS, Chemistry and Radiochemistry Group, Paris (France); Corredera, G. [Electricite de France, Centre d' Expertise et d' Inspection dans les domaines de la Realisation et de l' Exploitation, Saint-Denis (France)

2010-07-01

Deposit buildup which caused the clogging of the 'foils' of the upper tube-support-plates (TSP) inside a PWR steam generator of French NPPs in 2006 presents certain similarities with deposits observed in lab tests performed in secondary coolant chemistry at the Technical Centre of AREVA NP in 2002. The mechanism of TSP clogging seems not to present obvious phenomenological links with the fouling of the free span of SG since deposits buildup is quite uniform and is currently related to a surface boiling effect due to the surface heat flux. A specific mechanism could account for TSP clogging. In particular, electrokinetic effects were investigated by EDF-CEIDRE and AREVA NP SAS in the framework of a lab test program started in 2007. The electrokinetic approach is to consider that the coupling of local hydrodynamic and surface electrochemistry could lead to the formation of a very localized and heterogeneous deposit at the leading edge between both TSP and SG tubing material. Electrokinetic effects can lead to the oxidation and/or the precipitation of ferrous ions and to a variation of the electrokinetic potential which can produce strong attraction of iron oxide colloids. These electrokinetic effects are dependent of the T/H and local hydrodynamic conditions and surface electrochemistry explaining. The objective of this EDF-AREVA lab test program is to investigate the role of secondary chemistry coolant (pH, DH, N{sub 2}H{sub 4}, amine, redox) and of the nature of materials (SS, Ni base alloy) on deposit buildup. Properties of oxide surface and zeta potential of oxidized metallic materials have been also determined at temperature to understand their potential contribution on mechanism of TSP clogging in secondary side chemistry coolant. In this paper, a set of specific experiments carried out in this frame have been presented and discussed, paying particular attention to the effects of electrokinetic considerations and surface charges at oxide

Glycan elongation beyond the mucin associated Tn antigen protects tumor cells from immune-mediated killing

DEFF Research Database (Denmark)

Madsen, Caroline B; Lavrsen, Kirstine; Steentoft, Catharina

2013-01-01

are recognized as cancer associated truncated glycans, and are expressed in many adenocarcinomas, e.g. breast- and pancreatic cancer cells. To investigate the role of the cancer associated glycan truncations in immune-mediated killing we created glyco-engineered breast- and pancreatic cancer cells expressing...... only the shortest possible mucin-like glycans (Tn and STn). Glyco-engineering was performed by zinc finger nuclease (ZFN) knockout (KO) of the Core 1 enzyme chaperone COSMC, thereby preventing glycan elongation beyond the initial GalNAc residue in O-linked glycans. We find that COSMC KO in the breast...

An update on Lab Rover: A hospital material transporter

Science.gov (United States)

Mattaboni, Paul

1994-01-01

The development of a hospital material transporter, 'Lab Rover', is described. Conventional material transport now utilizes people power, push carts, pneumatic tubes and tracked vehicles. Hospitals are faced with enormous pressure to reduce operating costs. Cyberotics, Inc. developed an Autonomous Intelligent Vehicle (AIV). This battery operated service robot was designed specifically for health care institutions. Applications for the AIV include distribution of clinical lab samples, pharmacy drugs, administrative records, x-ray distribution, meal tray delivery, and certain emergency room applications. The first AIV was installed at Lahey Clinic in Burlington, Mass. Lab Rover was beta tested for one year and has been 'on line' for an additional 2 years.

[Hyperspectral remote sensing in monitoring the vegetation heavy metal pollution].

Science.gov (United States)

Li, Na; Lü, Jian-sheng; Altemann, W

2010-09-01

Mine exploitation aggravates the environment pollution. The large amount of heavy metal element in the drainage of slag from the mine pollutes the soil seriously, doing harm to the vegetation growing and human health. The investigation of mining environment pollution is urgent, in which remote sensing, as a new technique, helps a lot. In the present paper, copper mine in Dexing was selected as the study area and China sumac as the study plant. Samples and spectral data in field were gathered and analyzed in lab. The regression model from spectral characteristics for heavy metal content was built, and the feasibility of hyperspectral remote sensing in environment pollution monitoring was testified.

Geoaccumulation assessment of heavy metal pollution in Ikwo soils, eastern Nigeria.

Science.gov (United States)

Tyopine, Andrew A; Jayeoye, Titilope J; Okoye, Chukwuma O B

2018-01-04

An imbalance in the environment's composition leads to significant effect on human activities such as farming. Of importance are heavy metals which are introduced anthropogenically or naturally. This calls for environmental monitoring and subsequent remediation if needed. An environmental monitoring exercise was conducted on Ikwo soils of Ebonyi State, eastern Nigeria with the aim of determining concentration levels for possible remediation. A total of 18 soil composite samples taken at 0-50 cm below soil surface from fallowed and cultivated soils not fertilized were subjected to heavy metal analyses and fertility indices like: organic matter (OM), cation exchange capacity (CEC), % total nitrogen (%TN), organic carbon (OC), and salinity. A correlation at 95% confidence level between geo-accumulations (I geo ) of the various heavy metals with salinity, OM, and CEC of the sampled soils reveals that I geo could be a contributing factor to the fertility status of the soils. With the aid of inductively coupled plasma atomic emission spectrophotometer (ICP-AES), the distribution pattern was determined as Mn> Fe> Zn>Cu> Mo> Cd> V>Hg>Ti> Ni>Bi> Pb> Co>Ag>Au> Cr>Pd>Pt. The I geo of the heavy metals in the study area varied from heavily to extremely contaminated levels. A remediation exercise was recommended on Ikwo soils due to their high salinity level and low CEC.

Aerial view of the water reservoirs for Lab II

CERN Multimedia

1974-01-01

Two large reservoirs (5000 m3 each) were built on the Swiss part of the site (Lab I is on the left). The water was drawn from the pumping station at Le Vengeron on Lac Léman, through a 10 km long pipe to be distributed over all Lab II.

77 FR 21505 - Proposed Amendment of Class D and E Airspace; Blountville, TN, and Revocation of Class E Airspace...

Science.gov (United States)

2012-04-10

... Airport, VA (Lat. 36[deg]41'14'' N., long. 82[deg]02'00'' W.) Rogersville, Hawkins County Airport, TN (Lat... Highlands Airport and within a 7-mile radius of Hawkins County Airport, and within 7 miles each side of Runway 07/25 centerline, extending from the 7-mile radius to 12 miles east of Hawkins County Airport...

Accelerating Industrial Adoption of Metal Additive Manufacturing Technology

Science.gov (United States)

Vartanian, Kenneth; McDonald, Tom

2016-03-01

While metal additive manufacturing (AM) technology has clear benefits, there are still factors preventing its adoption by industry. These factors include the high cost of metal AM systems, the difficulty for machinists to learn and operate metal AM machines, the long approval process for part qualification/certification, and the need for better process controls; however, the high AM system cost is the main barrier deterring adoption. In this paper, we will discuss an America Makes-funded program to reduce AM system cost by combining metal AM technology with conventional computerized numerical controlled (CNC) machine tools. Information will be provided on how an Optomec-led team retrofitted a legacy CNC vertical mill with laser engineered net shaping (LENS®—LENS is a registered trademark of Sandia National Labs) AM technology, dramatically lowering deployment cost. The upgraded system, dubbed LENS Hybrid Vertical Mill, enables metal additive and subtractive operations to be performed on the same machine tool and even on the same part. Information on the LENS Hybrid system architecture, learnings from initial system deployment and continuing development work will also be provided to help guide further development activities within the materials community.

Application of DICOM Standard in LabVIEW Environment

Directory of Open Access Journals (Sweden)

Dušan KONIAR

2008-01-01

Full Text Available DICOM is the world standard for picture archiving and communication in medicine. Development system LabVIEW based on graphical programming is primary designed for virtual instrumentation, it offers many tools and operators for image processing and analysis, but it does not directly support the work with DICOM standard. The article deals with possibility of importing native DICOM files to LabVIEW and work with them.

PlanetLab Europe as Geographically-Distributed Testbed for Software Development and Evaluation

Directory of Open Access Journals (Sweden)

Dan Komosny

2015-01-01

Full Text Available In this paper, we analyse the use of PlanetLab Europe for development and evaluation of geographically-oriented Internet services. PlanetLab is a global research network with the main purpose to support development of new Internet services and protocols. PlanetLab is divided into several branches; one of them is PlanetLab Europe. PlanetLab Europe consists of about 350 nodes at 150 geographically different sites. The nodes are accessible by remote login, and the users can run their software on the nodes. In the paper, we study the PlanetLab's properties that are significant for its use as a geographically distributed testbed. This includes node position accuracy, services availability and stability. We find a considerable number of location inaccuracies and a number of services that cannot be considered as reliable. Based on the results we propose a simple approach to nodes selection in testbeds for geographically-oriented Internet services development and evaluation.

Distinguishing Truncated and Normal MUC1 Glycoform Targeting from Tn-MUC1-Specific CAR T Cells

DEFF Research Database (Denmark)

Posey, Avery D; Clausen, Henrik; June, Carl H

2016-01-01

Genetically modified T cells expressing chimeric antigen receptors (CARs) demonstrate potent clinical antitumor effects in a variety of blood cancers. However, clinical activity in solid tumors has been disappointing and toxicity has been a serious concern (Lamers et al., 2013; Morgan et al., 2010......). We recently found that a CAR composed of a scFv antibody fragment specific for the Tn-glycoform of MUC1 had potent activity in preclinical models of blood cancer and adenocarcinoma (Posey et al., 2016)....

78 FR 60245 - Privacy Act Systems of Records; LabWare Laboratory Information Management System

Science.gov (United States)

2013-10-01

... of Records; LabWare Laboratory Information Management System AGENCY: Animal and Plant Health... system of records, entitled LabWare Laboratory Information Management System (LabWare LIMS), to maintain... Affairs, OMB. Thomas J. Vilsack, Secretary. SYSTEM NAME: LabWare Laboratory Information Management System...

The community FabLab platform: applications and implications in biomedical engineering.

Science.gov (United States)

Stephenson, Makeda K; Dow, Douglas E

2014-01-01

Skill development in science, technology, engineering and math (STEM) education present one of the most formidable challenges of modern society. The Community FabLab platform presents a viable solution. Each FabLab contains a suite of modern computer numerical control (CNC) equipment, electronics and computing hardware and design, programming, computer aided design (CAD) and computer aided machining (CAM) software. FabLabs are community and educational resources and open to the public. Development of STEM based workforce skills such as digital fabrication and advanced manufacturing can be enhanced using this platform. Particularly notable is the potential of the FabLab platform in STEM education. The active learning environment engages and supports a diversity of learners, while the iterative learning that is supported by the FabLab rapid prototyping platform facilitates depth of understanding, creativity, innovation and mastery. The product and project based learning that occurs in FabLabs develops in the student a personal sense of accomplishment, self-awareness, command of the material and technology. This helps build the interest and confidence necessary to excel in STEM and throughout life. Finally the introduction and use of relevant technologies at every stage of the education process ensures technical familiarity and a broad knowledge base needed for work in STEM based fields. Biomedical engineering education strives to cultivate broad technical adeptness, creativity, interdisciplinary thought, and an ability to form deep conceptual understanding of complex systems. The FabLab platform is well designed to enhance biomedical engineering education.

A Simple, Successful Capacitor Lab

Science.gov (United States)

Ennis, William

2011-01-01

Capacitors are a fundamental component of modern electronics. They appear in myriad devices and in an enormous range of sizes. Although our students are taught the function and analysis of capacitors, few have the opportunity to use them in our labs.

CARACTERIZACIÓN DE IGYS ANTI-LECTINA DE Salvia bogotensis Y SU APLICACIÓN EN ESTUDIOS CITOQUÍMICOS PARA LA DETECCIÓN DEL ANTÍGENO TN

Directory of Open Access Journals (Sweden)

Nohora Vega

2010-02-01

Full Text Available Las inmunoglobulinas aisladas de la yema de huevo (IgY son muy utilizadas actualmente en diversos campos de las ciencias biológicas, dadas sus ventajas frente a las IgG séricas de mamíferos. En un trabajo previo establecimos las condiciones de obtención de IgY dirigidas contra la lectinas de Salvia bogotensis; su utilización en estudios inmunocitoquímicos requiere conocer sus principales características moleculares y las condiciones para la interacción IgY-lectina. La lectina de Salvia bogotensis (SBoL reconoce específicamente el antígeno Tn, marcador tumoral en muchos tipos de cáncer, pero se requieren herramientas adicionales para evidenciar esta interacción a nivel celular. Dada la disponibilidad de IgY anti-lectina de S. bogotensis, se realizó este trabajo con el objeto de caracterizar molecularmente estas IgY y evaluar su utilización en estudios inmunocitoquímicos para la detección del antígeno Tn en células tumorales. A las IgY purificadas se les determinó su punto isoeléctrico, peso molecular y contenido de carbohidratos. Para establecer la especificidad de interacción IgY-SBoL se obtuvieron lectinas homólogas y heterólogas y se ensayaron por ELLSA. La detección del antígeno Tn en las líneas celulares MCF-7 y HeLa con la lectina y las IgYs marcadas con biotina o peroxidasa se realizó por CELISA e inmunocitoquímica. Los resultados mostraron que los anticuerpos IgY anti-SBoL son una herramienta de una alta sensibilidad para los ensayos de reconocimiento específico del antígeno Tn.

Tumor-associated Tn-MUC1 glycoform is internalized through the macrophage galactose-type C-type lectin and delivered to the HLA class I and II compartments in dendritic cells

DEFF Research Database (Denmark)

Napoletano, Chiara; Rughetti, Aurelia; Agervig Tarp, Mads P

2007-01-01

. This results in the expression of tumor-associated glycoforms and in MUC1 carrying the tumor-specific glycan Tn (GalNAcalpha1-O-Ser/Thr). Glycopeptides corresponding to three tandem repeats of MUC1, enzymatically glycosylated with 9 or 15 mol of GalNAc, were shown to specifically bind and to be internalized...... and ELISA done on subcellular fractions of iDCs showed that the Tn-MUC1 glycopeptides colocalized with HLA class I and II compartments after internalization. Importantly, although Tn-MUC1 recombinant protein was bound and internalized by MGL, the glycoprotein entered the HLA class II compartment......, but not the HLA class I pathway. These data indicate that MGL expressed on iDCs is an optimal receptor for the internalization of short GalNAcs carrying immunogens to be delivered into HLA class I and II compartments. Such glycopeptides therefore represent a new way of targeting the HLA class I and II pathways...

Integration of MSFC Usability Lab with Usability Testing

Science.gov (United States)

Cheng, Yiwei; Richardson, Sally

2010-01-01

As part of the Stage Analysis Branch, human factors engineering plays an important role in relating humans to the systems of hardware and structure designs of the new launch vehicle. While many branches are involved in the technical aspects of creating a launch vehicle, human factors connects humans to the scientific systems with the goal of improving operational performance and safety while reducing operational error and damage to the hardware. Human factors engineers use physical and computerized models to visualize possible areas for improvements to ensure human accessibility to components requiring maintenance and that the necessary maintenance activities can be accomplished with minimal risks to human and hardware. Many methods of testing are used to fulfill this goal, such as physical mockups, computerized visualization, and usability testing. In this analysis, a usability test is conducted to test how usable a website is to users who are and are not familiar with it. The testing is performed using participants and Morae software to record and analyze the results. This analysis will be a preliminary test of the usability lab in preparation for use in new spacecraft programs, NASA Enterprise, or other NASA websites. The usability lab project is divided into two parts: integration of the usability lab and a preliminary test of the usability lab.

Berkeley Lab Laser Accelerator (BELLA) facility

Data.gov (United States)

Federal Laboratory Consortium — The Berkeley Lab Laser Accelerator (BELLA) facility (formerly LOASIS) develops advanced accelerators and radiation sources. High gradient (1-100 GV/m) laser-plasma...

Developing Guided Inquiry-Based Student Lab Worksheet for Laboratory Knowledge Course

Science.gov (United States)

Rahmi, Y. L.; Novriyanti, E.; Ardi, A.; Rifandi, R.

2018-04-01

The course of laboratory knowledge is an introductory course for biology students to follow various lectures practicing in the biology laboratory. Learning activities of laboratory knowledge course at this time in the Biology Department, Universitas Negeri Padang has not been completed by supporting learning media such as student lab worksheet. Guided inquiry learning model is one of the learning models that can be integrated into laboratory activity. The study aimed to produce student lab worksheet based on guided inquiry for laboratory knowledge course and to determine the validity of lab worksheet. The research was conducted using research and developmet (R&D) model. The instruments used in data collection in this research were questionnaire for student needed analysis and questionnaire to measure the student lab worksheet validity. The data obtained was quantitative from several validators. The validators consist of three lecturers. The percentage of a student lab worksheet validity was 94.18 which can be categorized was very good.

The implement of the interface between EPICS and LabVIEW

International Nuclear Information System (INIS)

Liu Jia; Wang Chunhong

2009-01-01

The control system of BEPCII (Beijing Electron Positron Collider) is based on EPICS (Experimental Physics and Industrial Control System). LabVIEW is often used to develop data acquisition system on Windows platform. EPICS IOC version 3.14 or later, can be currently running on windows. The SharedMemory developed by SNS (Spallation Neutron Source) can implement the interface between Windows IOC and LabVIEW. The paper describes the method how to use SharedMemory and a application of magnet measurement system, so that LabVIEW and EPICS can share data each other. (authors)

Advanced teaching labs in physics - celebrating progress; challenges ahead

Science.gov (United States)

Peterson, Richard

A few examples of optical physics experiments may help us first reflect on significant progress on how advanced lab initiatives may now be more effectively developed, discussed, and disseminated - as opposed to only 10 or 15 years back. Many cooperative developments of the last decade are having profound impacts on advanced lab workers and students. Central to these changes are the programs of the Advanced Laboratory Physics Association (ALPhA) (Immersions, BFY conferences), AAPT (advlab-l server, ComPADRE, apparatus competitions, summer workshops/sessions), APS (Reichert Award, FEd activities and sessions), and the Jonathan F. Reichert Foundation (ALPhA support and institution matched equipment grants for Immersion participants). Broad NSF support has helped undergird several of these initiatives. Two of the most significant challenges before this new advanced lab community are (a) to somehow enhance funding opportunities for teaching equipment and apparatus in an era of minimal NSF equipment support, and (b) to help develop a more complementary relationship between research-based advanced lab pedagogies and the development of fresh physics experiments that help enable the mentoring and experimental challenge of our students.

Gail Harlamoff: Executive Director, Life Lab Science Program

OpenAIRE

Rabkin, Sarah

2010-01-01

Gail Harlamoff is Executive Director of the Life Lab Science Program, a nationally recognized, award-winning nonprofit science and environmental organization located on the UC Santa Cruz campus. Founded in 1979, Life Lab helps schools develop gardens and implement curricula to enhance students’ learning about science, math, and the natural world. The program has trained tens of thousands of educators in more than 1400 schools across the country. Life Lab’s specialized initiatives inc...

Implementation of Siemens USS protocol into LabVIEW.

Science.gov (United States)

Hosek, P; Diblik, M

2011-10-01

This article gives basic overview of the USS protocol as a communication interface to drive Siemens frequency inverters. It presents our implementation of this protocol into LabVIEW, as there was permanent demand from the community of the users to have native LabVIEW implementation of the USS protocol. It also states encountered problems and their solutions. Copyright © 2011 Society for Laboratory Automation and Screening. Published by Elsevier Inc. All rights reserved.

EUSO@TurLab: An experimental replica of ISS orbits

Directory of Open Access Journals (Sweden)

Bertaina M.

2015-01-01

Full Text Available The EUSO@TurLab project is an on-going activity aimed to reproduce atmospheric and luminous conditions that JEM-EUSO will encounter on its orbits around the Earth. The use of the TurLab facility, part of the Department of Physics of the University of Torino, allows the simulation of different surface conditions in a very dark and rotating environment in order to test the response of JEM-EUSO's sensors and sensitivity. The experimental setup currently in operation has been used to check the potential of the TurLab facility for the above purposes, and the acquired data will be used to test the concept of JEM-EUSO's trigger system.

Photonics and Fiber Optics Processor Lab

Data.gov (United States)

Federal Laboratory Consortium — The Photonics and Fiber Optics Processor Lab develops, tests and evaluates high speed fiber optic network components as well as network protocols. In addition, this...

Learning Experience on Transformer Using HOT Lab for Pre-service Physics Teacher’s

Science.gov (United States)

Malik, A.; Setiawan, A.; Suhandi, A.; Permanasari, A.

2017-09-01

This study aimed at investigating pre-service teacher’s critical thinking skills improvement through Higher Order Thinking (HOT) Lab on transformer learning. This research used mix method with the embedded experimental model. Research subjects are 60 students of Physics Education in UIN Sunan Gunung Djati Bandung. The results showed that based on the results of the analysis of practical reports and observation sheet shows students in the experimental group was better in carrying out the practicum and can solve the real problem while the control group was going on the opposite. The critical thinking skills of students applying the HOT Lab were higher than the verification lab. Critical thinking skills could increase due to HOT Lab based problems solving that can develop higher order thinking skills through laboratory activities. Therefore, it was concluded that the application of HOT Lab was more effective than verification lab on improving students’ thinking skills on transformer topic learning. Finally, HOT Lab can be implemented in other subject learning and could be used to improve another higher order thinking skills.

Use of tablets for instruction and learning in microbiology labs

DEFF Research Database (Denmark)

Møller, Karen Louise; Jelsbak, Vibe Alopaeus; Georgsen, Marianne

of this project are to develop a technological infrastructure to support students’ work in the lab and to develop teaching and learning resources. Our research question is: How is teaching and learning in the laboratory influenced by the tablets and the following multimodal teaching and learning materials...... and taken notes by hand. Use of tablets in the lab offers new opportunities. In September 2012, nine tablets were introduced into one of the labs of the college. Groups of students use the tablets to access documents, watch video instructions, and to document results and procedures digitally. The objectives......? The empirical part of the project has been documented through field observations in the lab (in writing and with photos). We have found the following to be characteristic of the work of the students: the students use the tablets collaboratively, take more photos than requested, use the video based instructions...

A Big Bang Lab

Science.gov (United States)

Scheider, Walter

2005-01-01

The February 2005 issue of The Science Teacher (TST) reminded everyone that by learning how scientists study stars, students gain an understanding of how science measures things that can not be set up in lab, either because they are too big, too far away, or happened in a very distant past. The authors of "How Far are the Stars?" show how the…

Human Engineering Modeling and Performance Lab Study Project

Science.gov (United States)

Oliva-Buisson, Yvette J.

2014-01-01

The HEMAP (Human Engineering Modeling and Performance) Lab is a joint effort between the Industrial and Human Engineering group and the KAVE (Kennedy Advanced Visualiations Environment) group. The lab consists of sixteen camera system that is used to capture human motions and operational tasks, through te use of a Velcro suit equipped with sensors, and then simulate these tasks in an ergonomic software package know as Jac, The Jack software is able to identify the potential risk hazards.

Observation of a Slater-type metal-to-insulator transition in Sr$_2$IrO$_4$ from time-resolved photo-carrier dynamics

OpenAIRE

Hsieh, D.; Mahmood, F.; Torchinsky, D. H.; Cao, G.; Gedik, N.

2012-01-01

We perform a time-resolved optical study of Sr$_2$IrO$_4$ to understand the influence of magnetic ordering on the low energy electronic structure of a strongly spin-orbit coupled $J_{eff}$=1/2 Mott insulator. By studying the recovery dynamics of photo-carriers excited across the Mott gap, we find that upon cooling through the N\\'{e}el temperature $T_N$ the system evolves continuously from a metal-like phase with fast ($\\sim$50 fs) and excitation density independent relaxation dynamics to a ga...

Promoting Metacognition in Introductory Calculus-based Physics Labs

Science.gov (United States)

Grennell, Drew; Boudreaux, Andrew

2010-10-01

In the Western Washington University physics department, a project is underway to develop research-based laboratory curriculum for the introductory calculus-based course. Instructional goals not only include supporting students' conceptual understanding and reasoning ability, but also providing students with opportunities to engage in metacognition. For the latter, our approach has been to scaffold reflective thinking with guided questions. Specific instructional strategies include analysis of alternate reasoning presented in fictitious dialogues and comparison of students' initial ideas with their lab group's final, consensus understanding. Assessment of student metacognition includes pre- and post- course data from selected questions on the CLASS survey, analysis of written lab worksheets, and student opinion surveys. CLASS results are similar to a traditional physics course and analysis of lab sheets show that students struggle to engage in a metacognitive process. Future directions include video studies, as well as use of additional written assessments adapted from educational psychology.

[Establishment of Reference Value of Hs-cTnT in Sichuan Region and Its Diagnostic Value in Patients with Chest Pain].

Science.gov (United States)

Guo, Ying; Du, Xiang-Yang; Huang, Hua-Lan; Wang, Wei-Qing; Nie, Xin; Li, Gui-Xing

2017-11-01

To establish the reference value of high sensitive cardiac troponin T (hs-cTnT) and the efficiency of reference value in the diagnosis of chest pain. Volunteers from eight independent communities in Chengdu,Sichuan were selected with detailed records of physical examination,electrocardiogram,ultrasound examination. The level of hs-cTnT for healthy volunteers was tested to determine ninety-ninth percentile references according to sex and ages. 2 249 patients with chest pain in the emergency department of Western China Hospital from July 2009 to July 2014 were enrolled to measure the efficiency of reference value for diagnosing acute myocardial infarction (AMI). There were 1 305 volunteers included finally. Among them,the mean hs-cTnT level of male was 4.3 (3.2-5.9) ng/L,which was significantly higher than that of female 3.0 (3.0-3.1) ng/L ( P value was less than 0.01. The 99th percentile values of male were 10.8 ng/L,15.4 ng/L and 19.7 ng/L for values of female were 4.6 ng/L,8.9 ng/L,18.8 ng/L,respectively. There was no difference in sensitivity and specificity between the value we figured out and manufactures provided (14.0 ng/L) for thosevalue did not show diversity ( P >0.05) but the specificity and positive predictive value showed significant difference (male: 0.67 vs. 0.56 and 0.83 vs. 0.79, P value of patients<60 yr. should be 14.0 ng/L,while 20.0 ng/L for those patients≥60 yr.

MATLAB-Like Scripting of Java Scientific Libraries in ScalaLab

Directory of Open Access Journals (Sweden)

Stergios Papadimitriou

2014-01-01

Full Text Available Although there are a lot of robust and effective scientific libraries in Java, the utilization of these libraries in pure Java is difficult and cumbersome, especially for the average scientist that does not expertise in software development. We illustrate that ScalaLab presents an easier and productive MATLAB like front end. Also, the main strengths and weaknesses of the core Java libraries of ScalaLab are elaborated. Since performance is of paramount importance for scientific computation, the article discusses extensively performance aspects of the ScalaLab environment. Also, Java bytecode performance is compared to native code.

Arduino-Based embedded systems interfacing, simulation, and LabVIEW GUI

CERN Document Server

Singh, Rajesh; Singh, Bhupendra; Choudhury, Sushabhan

2018-01-01

Arduino is an open-source electronics platform based on easy-to-use hardware and software while LabVIEW is a graphical programming telling how to connect functions and work with a variety of datatypes when constructing applications.This book will help beginners to get started with Arduino-based embedded systems including essential know-how of the programming and interfacing of the devices. Book includes programming and simulation of Arduino-based projects and interfacing with LabVIEW, based on practical case studies. The book comprises of total twenty five chapters with description, working model of LabVIEW and programming with Arduino IDE.

Airborne Low-Frequency Sonar (ALFS) Lab

Data.gov (United States)

Federal Laboratory Consortium — The ALFS lab is dedicated to support acoustic data analysis and processing software support to the AN/AQS-22 dipping sonar system. It includes stand-alone Software...

Generator Inspection Report: Bio - Lab, Inc.

Science.gov (United States)

Contains report from Georgia Department of Natural Resources of July 21, 1999 inspection of the Bio - Lab Incorporated Plant 4 in Conyers, Rockdale County, Georgia, reporting that no violations were observed.

Development of an accelerator-based BNCT facility at the Berkeley Lab

International Nuclear Information System (INIS)

Ludewigt, B.A.; Bleuel, D.; Chu, W.T.; Donahue, R.J.; Kwan, J.; Reginato, L.L.; Wells, R.P.

1998-01-01

An accelerator-based BNCT facility is under construction at the Berkeley Lab. An electrostatic-quadrupole (ESQ) accelerator is under development for the production of neutrons via the 7 Li(p,n) 7 Be reaction at proton energies between 2.3 and 2.5 MeV. A novel type of power supply, an air-core coupled transformer power supply, is being built for the acceleration of beam currents exceeding 50 mA. A metallic lithium target has been developed for handling such high beam currents. Moderator, reflector and neutron beam delimiter have extensively been modeled and designs have been identified which produce epithermal neutron spectra sharply peaked between 10 and 20 keV. These. neutron beams are predicted to deliver significantly higher doses to deep seated brain tumors, up to 50% more near the midline of the brain than is possible with currently available reactor beams. The accelerator neutron source will be suitable for future installation at hospitals

ATC-lab(Advanced): an air traffic control simulator with realism and control.

Science.gov (United States)

Fothergill, Selina; Loft, Shayne; Neal, Andrew

2009-02-01

ATC-lab(Advanced) is a new, publicly available air traffic control (ATC) simulation package that provides both realism and experimental control. ATC-lab(Advanced) simulations are realistic to the extent that the display features (including aircraft performance) and the manner in which participants interact with the system are similar to those used in an operational environment. Experimental control allows researchers to standardize air traffic scenarios, control levels of realism, and isolate specific ATC tasks. Importantly, ATC-lab(Advanced) also provides the programming control required to cost effectively adapt simulations to serve different research purposes without the need for technical support. In addition, ATC-lab(Advanced) includes a package for training participants and mathematical spreadsheets for designing air traffic events. Preliminary studies have demonstrated that ATC-lab(Advanced) is a flexible tool for applied and basic research.

Elaboration and quality control of the inoculum of the experimental vaccine Brucella S19-tn7-GFP for use in white animals and associated serological test for the detection of anti-GFP antibodies

International Nuclear Information System (INIS)

Salas Alfaro, Dariana

2014-01-01

The preparation of the inoculum of the experimental vaccine Brucella S19-Tn7-GFP is optimized for application in white animals. An associated serological test has allowed differentiating infected animals from those vaccinated with the experimental strain. The same bacteriological and biological properties of the B. abortus S19-Tn7-GFP strain have maintained in the parental vaccine strain S19 and is stable over time. A protocol for the inoculums of strain S19-Tn7-GFP is established for its preparation and use in white animals and quality control. The inoculum stability is evaluated through the simulation of conditions that can be presented in the transportation and application process in the field. An enzyme immunoassay ELISA is optimized for the detection of anti-GFP antibodies in cattle [es

The Design of NetSecLab: A Small Competition-Based Network Security Lab

Science.gov (United States)

Lee, C. P.; Uluagac, A. S.; Fairbanks, K. D.; Copeland, J. A.

2011-01-01

This paper describes a competition-style of exercise to teach system and network security and to reinforce themes taught in class. The exercise, called NetSecLab, is conducted on a closed network with student-formed teams, each with their own Linux system to defend and from which to launch attacks. Students are expected to learn how to: 1) install…

Attracting STEM talent: do STEM students prefer traditional or work/life-interaction labs?

Science.gov (United States)

DeFraine, William C; Williams, Wendy M; Ceci, Stephen J

2014-01-01

The demand for employees trained in science, technology, engineering, and mathematics (STEM) fields continues to increase, yet the number of Millennial students pursuing STEM is not keeping pace. We evaluated whether this shortfall is associated with Millennials' preference for flexibility and work/life-interaction in their careers-a preference that may be inconsistent with the traditional idea of a science career endorsed by many lab directors. Two contrasting approaches to running STEM labs and training students were explored, and we created a lab recruitment video depicting each. The work-focused video emphasized the traditional notions of a science lab, characterized by long work hours and a focus on individual achievement and conducting research above all else. In contrast, the work/life-interaction-focused video emphasized a more progressive view - lack of demarcation between work and non-work lives, flexible hours, and group achievement. In Study 1, 40 professors rated the videos, and the results confirmed that the two lab types reflected meaningful real-world differences in training approaches. In Study 2, we recruited 53 current and prospective graduate students in STEM fields who displayed high math-identification and a commitment to science careers. In a between-subjects design, they watched one of the two lab-recruitment videos, and then reported their anticipated sense of belonging to and desire to participate in the lab depicted in the video. Very large effects were observed on both primary measures: Participants who watched the work/life-interaction-focused video reported a greater sense of belonging to (d = 1.49) and desire to participate in (d = 1.33) the lab, relative to participants who watched the work-focused video. These results suggest Millennials possess a strong desire for work/life-interaction, which runs counter to the traditional lab-training model endorsed by many lab directors. We discuss implications of these findings for STEM

Undergraduates in the lab: Analyzing metal and organic contaminants in oysters and sediments from southeastern North Carolina

Science.gov (United States)

Mead, R. N.; Kipp, L. E.; Liberatore, H.; Sherard, S.; Steagall, M.; Skrabal, S. A.

2016-02-01

A state-funded project to analyze a suite of metal and organic contaminants in oyster tissues and ambient sediments was carried out nearly exclusively by over 10 undergraduates at the University of North Carolina Wilmington. This study will present Concentrations of various trace metals (most notably arsenic, copper, mercury, and zinc) and organic contaminants (polycyclic aromatic hydrocarbons and the antibacterial, triclosan) have been determined in oyster tissues and adjacent sediments in New Hanover and Brunswick counties, southeastern North Carolina. Trace metals that exceeded national median levels at multiple sites in this study included arsenic, copper, and zinc. Elevated levels of arsenic (exceeding the national median and, often, the national 85th percentiles) in oyster tissues are characteristic of much of the southeastern United States; these elevations are attributed to high natural background levels in the underlying bedrock and sediments as well as historical contamination by arsenic-containing agricultural pesticides. Another metal of national concern is mercury; however, concentrations of this metal were mostly at the national median for oyster tissue. Polycyclic aromatic hydrocarbons (PAHs) barely exceeded or were near the national median at only 3 sites, 2 in Lockwood Folly estuary, Brunswick County and 1 at Bradley Creek, New Hanover County. Concentrations at the remaining sites were 4 to >10 times less than the national median. Triclosan, an antibacterial compound used in many consumer products, was found in oyster tissues and sediments at the 4 sites at which it was examined. Oyster tissues contained triclosan at levels 2 to 43 times as high as adjacent sediments, indicating its bioaccumulation potential. Levels of metals and PAHs in oyster tissues are consistently elevated near more urbanized areas but are unlikely to be at levels harmful for human consumption.

The androgen receptor controls expression of the cancer-associated sTn antigen and cell adhesion through induction of ST6GalNAc1 in prostate cancer

Science.gov (United States)

Munkley, Jennifer; Oltean, Sebastian; Vodák, Daniel; Wilson, Brian T.; Livermore, Karen E.; Zhou, Yan; Star, Eleanor; Floros, Vasileios I.; Johannessen, Bjarne; Knight, Bridget; McCullagh, Paul; McGrath, John; Crundwell, Malcolm; Skotheim, Rolf I.; Robson, Craig N.; Leung, Hing Y.; Harries, Lorna W.; Rajan, Prabhakar; Mills, Ian G.; Elliott, David J.

2015-01-01

Patterns of glycosylation are important in cancer, but the molecular mechanisms that drive changes are often poorly understood. The androgen receptor drives prostate cancer (PCa) development and progression to lethal metastatic castration-resistant disease. Here we used RNA-Seq coupled with bioinformatic analyses of androgen-receptor (AR) binding sites and clinical PCa expression array data to identify ST6GalNAc1 as a direct and rapidly activated target gene of the AR in PCa cells. ST6GalNAc1 encodes a sialytransferase that catalyses formation of the cancer-associated sialyl-Tn antigen (sTn), which we find is also induced by androgen exposure. Androgens induce expression of a novel splice variant of the ST6GalNAc1 protein in PCa cells. This splice variant encodes a shorter protein isoform that is still fully functional as a sialyltransferase and able to induce expression of the sTn-antigen. Surprisingly, given its high expression in tumours, stable expression of ST6GalNAc1 in PCa cells reduced formation of stable tumours in mice, reduced cell adhesion and induced a switch towards a more mesenchymal-like cell phenotype in vitro. ST6GalNAc1 has a dynamic expression pattern in clinical datasets, being significantly up-regulated in primary prostate carcinoma but relatively down-regulated in established metastatic tissue. ST6GalNAc1 is frequently upregulated concurrently with another important glycosylation enzyme GCNT1 previously associated with prostate cancer progression and implicated in Sialyl Lewis X antigen synthesis. Together our data establishes an androgen-dependent mechanism for sTn antigen expression in PCa, and are consistent with a general role for the androgen receptor in driving important coordinate changes to the glycoproteome during PCa progression. PMID:26452038

Automated Measurement and Signaling Systems for the Transactional Network

Energy Technology Data Exchange (ETDEWEB)

Piette, Mary Ann; Brown, Richard; Price, Phillip; Page, Janie; Granderson, Jessica; Riess, David; Czarnecki, Stephen; Ghatikar, Girish; Lanzisera, Steven

2013-12-31

The Transactional Network Project is a multi-lab activity funded by the US Department of Energy?s Building Technologies Office. The project team included staff from Lawrence Berkeley National Laboratory, Pacific Northwest National Laboratory and Oak Ridge National Laboratory. The team designed, prototyped and tested a transactional network (TN) platform to support energy, operational and financial transactions between any networked entities (equipment, organizations, buildings, grid, etc.). PNNL was responsible for the development of the TN platform, with agents for this platform developed by each of the three labs. LBNL contributed applications to measure the whole-building electric load response to various changes in building operations, particularly energy efficiency improvements and demand response events. We also provide a demand response signaling agent and an agent for cost savings analysis. LBNL and PNNL demonstrated actual transactions between packaged rooftop units and the electric grid using the platform and selected agents. This document describes the agents and applications developed by the LBNL team, and associated tests of the applications.

A Series of Computational Neuroscience Labs Increases Comfort with MATLAB.

Science.gov (United States)

Nichols, David F

2015-01-01

Computational simulations allow for a low-cost, reliable means to demonstrate complex and often times inaccessible concepts to undergraduates. However, students without prior computer programming training may find working with code-based simulations to be intimidating and distracting. A series of computational neuroscience labs involving the Hodgkin-Huxley equations, an Integrate-and-Fire model, and a Hopfield Memory network were used in an undergraduate neuroscience laboratory component of an introductory level course. Using short focused surveys before and after each lab, student comfort levels were shown to increase drastically from a majority of students being uncomfortable or with neutral feelings about working in the MATLAB environment to a vast majority of students being comfortable working in the environment. Though change was reported within each lab, a series of labs was necessary in order to establish a lasting high level of comfort. Comfort working with code is important as a first step in acquiring computational skills that are required to address many questions within neuroscience.

City Labs as Vehicles for Innovation in Urban Planning Processes

Directory of Open Access Journals (Sweden)

Christian Scholl

2016-12-01

Full Text Available This paper assesses the role of urban experiments for local planning processes through a case-based analysis of the city lab of Maastricht. In conjunction with this, the article offers three contributions, as additional elements. Firstly, the paper develops a set of defining characteristics of city labs as an analytical concept which is relevant for discussions about (collaborative planning. Secondly, it refines the literature on collaborative planning by drawing attention to experimentation and innovation. Thirdly, the paper assesses the potential of city labs to contribute to the innovation of urban governance. The work draws from the literature on experimentation and learning as well as the literature on collaborative urban planning. In the conclusions, we discuss the potential of city labs as vehicles for learning about new urban planning approaches and their limitations as spaces for small-scale experimentation. The paper is based on research for the URB@Exp research project funded by JPI Urban Europe.

Heat transfer virtual lab for students and engineers theory and guide for setting up

CERN Document Server

Fridman, Ella

2014-01-01

Laboratory experiments are a vital part of engineering education,which historically were considered impractical for distance learning.This book presents a guide for the practical employment of a heattransfer virtual lab for students and engineers.Inside, the authors have detailed this virtual lab which is designedand can implement a real-time, robust, and scalable software systemthat provides easy access to lab equipment anytime and anywhereover the Internet. They introduce and explain LabVIEW ineasy-to-understand language. LabVIEW is a proprietary softwaretool by National Instruments, and can

Lab notebooks as scientific communication: Investigating development from undergraduate courses to graduate research

Directory of Open Access Journals (Sweden)

Jacob T. Stanley

2016-09-01

Full Text Available In experimental physics, lab notebooks play an essential role in the research process. For all of the ubiquity of lab notebooks, little formal attention has been paid to addressing what is considered “best practice” for scientific documentation and how researchers come to learn these practices in experimental physics. Using interviews with practicing researchers, namely, physics graduate students, we explore the different experiences researchers had in learning how to effectively use a notebook for scientific documentation. We find that very few of those interviewed thought that their undergraduate lab classes successfully taught them the benefit of maintaining a lab notebook. Most described training in lab notebook use as either ineffective or outright missing from their undergraduate lab course experience. Furthermore, a large majority of those interviewed explained that they did not receive any formal training in maintaining a lab notebook during their graduate school experience and received little to no feedback from their advisors on these records. Many of the interviewees describe learning the purpose of, and how to maintain, these kinds of lab records only after having a period of trial and error, having already started doing research in their graduate program. Despite the central role of scientific documentation in the research enterprise, these physics graduate students did not gain skills in documentation through formal instruction, but rather through informal hands-on practice.

A Virtual PV Systems Lab for Engineering Undergraduate Curriculum

Directory of Open Access Journals (Sweden)

Emre Ozkop

2014-01-01

Full Text Available Design and utilization of a Virtual Photovoltaic Systems Laboratory for undergraduate curriculum are introduced in this paper. The laboratory introduced in this study is developed to teach students the basics and design steps of photovoltaic solar energy systems in a virtual environment before entering the field. The users of the proposed virtual lab will be able to determine the sizing by selecting related parameters of the photovoltaic system to meet DC and AC loading conditions. Besides, the user will be able to analyze the effect of changing solar irradiation and temperature levels on the operating characteristics of the photovoltaic systems. Common DC bus concept and AC loading conditions are also included in the system by utilizing a permanent magnet DC motor and an RLC load as DC and AC loading examples, respectively. The proposed Virtual Photovoltaic Systems Laboratory is developed in Matlab/Simulink GUI environment. The proposed virtual lab has been used in Power Systems Lab in the Department of Electrical and Electronics Engineering at Karadeniz Technical University as a part of undergraduate curriculum. A survey on the students who took the lab has been carried out and responses are included in this paper.

Lab-on-a-Chip Pathogen Sensors for Food Safety

Directory of Open Access Journals (Sweden)

Bumsang Kim

2012-08-01

Full Text Available There have been a number of cases of foodborne illness among humans that are caused by pathogens such as Escherichia coli O157:H7, Salmonella typhimurium, etc. The current practices to detect such pathogenic agents are cell culturing, immunoassays, or polymerase chain reactions (PCRs. These methods are essentially laboratory-based methods that are not at all real-time and thus unavailable for early-monitoring of such pathogens. They are also very difficult to implement in the field. Lab-on-a-chip biosensors, however, have a strong potential to be used in the field since they can be miniaturized and automated; they are also potentially fast and very sensitive. These lab-on-a-chip biosensors can detect pathogens in farms, packaging/processing facilities, delivery/distribution systems, and at the consumer level. There are still several issues to be resolved before applying these lab-on-a-chip sensors to field applications, including the pre-treatment of a sample, proper storage of reagents, full integration into a battery-powered system, and demonstration of very high sensitivity, which are addressed in this review article. Several different types of lab-on-a-chip biosensors, including immunoassay- and PCR-based, have been developed and tested for detecting foodborne pathogens. Their assay performance, including detection limit and assay time, are also summarized. Finally, the use of optical fibers or optical waveguide is discussed as a means to improve the portability and sensitivity of lab-on-a-chip pathogen sensors.

Living labs design and assessment of sustainable living

CERN Document Server

Guerra-Santin, Olivia; Lockton, Dan

2017-01-01

This book presents the results of a multi-annual project with sustainable Living Labs in the United Kingdom, Sweden, Germany and the Netherlands. Living Labs – as initiated by the authors – have proved to be very promising research, design, co-creation and communication facilities for the development and implementation of sustainable innovations in the home. The book provides an inspiring introduction to both the methodology and business modelling for the Living Lab facilities. Understanding daily living at home is key to designing products and services that support households in their transition to more sustainable lifestyles. This book not only explores new ways of gaining insights into daily practices, but also discusses developing and testing design methods to create sustainable solutions for households. These new methods and tools are needed because those available are either ineffective or cause rebound-effects. Intended for researchers and designers with an interest in the transition to sustainable...

Immunization of breast cancer patients using a synthetic sialyl-Tn glycoconjugate plus Detox adjuvant.

Science.gov (United States)

MacLean, G D; Reddish, M; Koganty, R R; Wong, T; Gandhi, S; Smolenski, M; Samuel, J; Nabholtz, J M; Longenecker, B M

1993-01-01

We have synthesized various formulations that have potential for active specific immunotherapy (ASI) of human cancers. Sialyl-Tn (STn) is a potentially important target structure for ASI because its expression on mucins is a strong, independent predictor of poor prognosis, suggesting that it may have functional significance in the metastatic process. In this first pilot study of synthetic sialyl-Tn hapten conjugated to keyhole limpet hemocyanin (STn-KLH), with Detox adjuvant, toxicity and humoral immunogenicity were assessed in 12 patients with metastatic breast cancer. Toxicity was minimal, restricted to local cutaneous reactions (apart from transient nausea and vomiting following single low-dose cyclophosphamide treatment). Using STn-conjugated human serum albumin in a solid-phase enzyme-linked immunosorbent assay, it was shown that all patients developed IgM and IgG specific for the synthetic STn hapten. Following immunization, most patients were shown to develop increased titres of complement-mediated cytotoxic antibodies, partially inhibited by synthetic STn hapten, but not by the related TF hapten. We also detected IgM and IgG antibodies reactive with natural STn determinants expressed on ovine submaxillary mucin, the STn specificity of this reactivity being confirmed by hapten inhibition. Evaluation of clinical efficacy in a small pilot study is difficult. Five patients are alive 12 or more months after entry, and another 4 patients are alive 6 or more months after entry into the study. All 3 patients with known widespread bulky disease progressed despite ASI, 2 having died from widespread cancer. Two patients had partial responses, each lasting 6 months. While several patients had disease stability for 3-10 months, 1 patient with pulmonary metastases remains stable 15 months after entry into the program.

The Earth is our lab: Ten years of geoscience school lab in Potsdam

Science.gov (United States)

Nikolaus Küppers, Andreas

2016-04-01

Starting in 2004, a geoscientific school lab for senior high school students was developed in the historical "Großer Refraktor" premises on the Telegraphenberg in Potsdam. Based on a one-day course architecture, laboratory days were developed covering singular themes: - Magnetic field of the Earth - Geographical Information Systems and geodata - Gravity field of the Earth - Geodynamics: seismology and seismics - Geoscience math - Geodata Brandenburg (Geological mapping with aerophotographs, remote sensing, underground data processing) With a focus on geophysical methodologies, course days generally focused on the field work around the Telegraphenberg site while introducing into the art of handling original professional equipment. Field data were afterwards compiled, analysed and interpreted in the group. Single days could be combined as clusters of up to one week and were bookable for national and international groups of max. 25 students. The courses were taught by active scientists with the assistance of student guides as the larger groups had to be split up. The paper gives an overview over the development history of the school lab and explains the course contents, the teaching methods and several employed escorting measures. Possible impact on the professional career decisions of the students is discussed.

Implementation of a Mobile Accessible Remote Lab

Directory of Open Access Journals (Sweden)

Danilo Garbi Zutin

2008-07-01

Full Text Available The purpose of the proposed research is to designand implement a LabVIEW-based remote lab client to runon a TCP/IP enabled PDA (Personal Digital Assistantdevice, thus teaching using this wireless m-learning systemwill not be limited by time and location. In addition,resources and equipments can be integrated and shared tothe extent that critically events can be monitored andhandled in time. An environment will be created to trainstudents to handle factory automation, data acquisition,data management, and manufacturing processes usingmobile devices. Furthermore, the integration and sharing oflab equipments via the Internet is a kind of teachingenvironment which promotes learning interests andefficiency using mobile devices.

Safety and shielding management for pulse power lab at IPR

International Nuclear Information System (INIS)

Upadhyay, Shweta; Faldu, Akash; Koshti, Rahul; Kumar, Rajesh

2016-01-01

Experiments in pulsed power lab works with very high voltage and high current regime for the nanosecond to microsecond time scale. This produces lot of electromagnetic noise, which can cause interference or malfunctioning of equipment. Laboratory Safety and protection are a very important aspect of science and engineering. Without it, practical performance could result in very serious injury, if not death. To reduce its effect electromagnetic shielding and grounding has to be enforced effectively. Pulse power lab deals with many safety issues like Radiation safety (shielding), High voltage safety, electrical and mechanical safety, etc. In this paper radiation all the safety aspects in pulse power lab is described. (author)

Perspectives on Industrial Innovation from Agilent, HP, and Bell Labs

Science.gov (United States)

Hollenhorst, James

2014-03-01

Innovation is the life blood of technology companies. I will give perspectives gleaned from a career in research and development at Bell Labs, HP Labs, and Agilent Labs, from the point of view of an individual contributor and a manager. Physicists bring a unique set of skills to the corporate environment, including a desire to understand the fundamentals, a solid foundation in physical principles, expertise in applied mathematics, and most importantly, an attitude: namely, that hard problems can be solved by breaking them into manageable pieces. In my experience, hiring managers in industry seldom explicitly search for physicists, but they want people with those skills.

Linear and exponential TAIL-PCR: a method for efficient and quick amplification of flanking sequences adjacent to Tn5 transposon insertion sites.

Science.gov (United States)

Jia, Xianbo; Lin, Xinjian; Chen, Jichen

2017-11-02

Current genome walking methods are very time consuming, and many produce non-specific amplification products. To amplify the flanking sequences that are adjacent to Tn5 transposon insertion sites in Serratia marcescens FZSF02, we developed a genome walking method based on TAIL-PCR. This PCR method added a 20-cycle linear amplification step before the exponential amplification step to increase the concentration of the target sequences. Products of the linear amplification and the exponential amplification were diluted 100-fold to decrease the concentration of the templates that cause non-specific amplification. Fast DNA polymerase with a high extension speed was used in this method, and an amplification program was used to rapidly amplify long specific sequences. With this linear and exponential TAIL-PCR (LETAIL-PCR), we successfully obtained products larger than 2 kb from Tn5 transposon insertion mutant strains within 3 h. This method can be widely used in genome walking studies to amplify unknown sequences that are adjacent to known sequences.

A breakthrough biosorbent in removing heavy metals: Equilibrium, kinetic, thermodynamic and mechanism analyses in a lab-scale study

Energy Technology Data Exchange (ETDEWEB)

Abdolali, Atefeh [Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Broadway, NSW 2007 (Australia); Ngo, Huu Hao, E-mail: h.ngo@uts.edu.au [Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Broadway, NSW 2007 (Australia); Guo, Wenshan [Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Broadway, NSW 2007 (Australia); Lu, Shaoyong [Chinese Research Academy of Environmental Science, Beijing 100012 (China); Chen, Shiao-Shing; Nguyen, Nguyen Cong [Institute of Environmental Engineering and Management, National Taipei University of Technology, No. 1, Sec. 3, Chung-Hsiao E. Rd, Taipei 106, Taiwan (China); Zhang, Xinbo [Department of Environmental and Municipal Engineering, Tianjin Key Laboratory of Aquatic Science and Technology, Tianjin Chengjian University, Jinjing Road 26, Tianjin 300384 (China); Wang, Jie; Wu, Yun [School of Environmental and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300387 (China)

2016-01-15

A breakthrough biosorbent namely multi-metal binding biosorbent (MMBB) made from a combination of tea wastes, maple leaves and mandarin peels, was prepared to evaluate their biosorptive potential for removal of Cd(II), Cu(II), Pb(II) and Zn(II) from multi-metal aqueous solutions. FTIR and SEM were conducted, before and after biosorption, to explore the intensity and position of the available functional groups and changes in adsorbent surface morphology. Carboxylic, hydroxyl and amine groups were found to be the principal functional groups for the sorption of metals. MMBB exhibited best performance at pH 5.5 with maximum sorption capacities of 31.73, 41.06, 76.25 and 26.63 mg/g for Cd(II), Cu(II), Pb(II) and Zn(II), respectively. Pseudo-first and pseudo-second-order models represented the kinetic experimental data in different initial metal concentrations very well. Among two-parameter adsorption isotherm models, the Langmuir equation gave a better fit of the equilibrium data. For Cu(II) and Zn(II), the Khan isotherm describes better biosorption conditions while for Cd(II) and Pb(II), the Sips model was found to provide the best correlation of the biosorption equilibrium data. The calculated thermodynamic parameters indicated feasible, spontaneous and exothermic biosorption process. Overall, this novel MMBB can effectively be utilized as an adsorbent to remove heavy metal ions from aqueous solutions. - Highlights: • A novel multi-metal binding biosorbent (MMBB) was studied. • The biosorption of Cd{sup 2+}, Cu{sup 2+}, Pb{sup 2+} and Zn{sup 2+} on MMBB was evaluated. • Hydroxyl, carbonyl and amine groups are involved in metal binding of MMBB. • Equilibrium data were presented and the best fitting models were identified. • The obtained results recommend this MMBB as potentially low-cost biosorbent.

The Jefferson Lab Trigger Supervisor System

International Nuclear Information System (INIS)

Ed Jastrzembsi; David Abbott; Graham Heyes; R.W. MacLeod; Carl Timmer; Elliott Wolin

2000-01-01

We discuss the design and performance of a Trigger Supervisor System for use in nuclear physics experiments at Jefferson Lab. We also discuss the enhanced features of a new Trigger Supervisor Module now under construction

Cockle Temperature Exposure Lab Experiment (2016)

Data.gov (United States)

U.S. Environmental Protection Agency — We carried out a lab experiment in which we exposed cockles to a range of air temperatures to simulate the physiological rigors of exposure to sunlight and air at...

Porcine wet lab improves surgical skills in third year medical students.

Science.gov (United States)

Drosdeck, Joseph; Carraro, Ellen; Arnold, Mark; Perry, Kyle; Harzman, Alan; Nagel, Rollin; Sinclair, Lynnsay; Muscarella, Peter

2013-09-01

Medical students desire to become proficient in surgical techniques and believe their acquisition is important. However, the operating room is a challenging learning environment. Small group procedural workshops can improve confidence, participation, and performance. The use of fresh animal tissues has been rated highly among students and improves their surgical technique. Greater exposure to surgical procedures and staff could positively influence students' interest in surgical careers. We hypothesized that a porcine "wet lab" course for third year medical students would improve their surgical skills. Two skills labs were conducted for third year medical students during surgery clerkships in the fall of 2011. The students' surgical skills were first evaluated in the operating room across nine dimensions. Next, the students performed the following procedures during the skills lab: (1) laparotomy; (2) small bowel resection; (3) splenectomy; (4) partial hepatectomy; (5) cholecystectomy; (6) interrupted abdominal wall closure; (7) running abdominal wall closure; and (8) skin closure. After the skills lab, the students were re-evaluated in the operating room across the same nine dimensions. Student feedback was also recorded. Fifty-one participants provided pre- and post-lab data for use in the final analysis. The mean scores for all nine surgical skills improved significantly after participation in the skills lab (P ≤ 0.002). Cumulative post-test scores also showed significant improvement (P = 0.002). Finally, the student feedback was largely positive. The surgical skills of third year medical students improved significantly after participation in a porcine wet lab, and the students rated the experience as highly educational. Integration into the surgery clerkship curriculum would promote surgical skill proficiency and could elicit interest in surgical careers. Copyright © 2013 Elsevier Inc. All rights reserved.

The Jefferson Lab Trigger Supervisor System

International Nuclear Information System (INIS)

Jastrzembski, E.; Abbott, D.J.; Heyes, W.G.; MacLeod, R.W.; Timmer, C.; Wolin, E.

1999-01-01

The authors discuss the design and performance of a Trigger Supervisor System for use in nuclear physics experiments at Jefferson Lab. They also discuss the enhanced features of a new Trigger Supervisor Module now under construction

Virtual Lab Demonstrations Improve Students’ Mastery of Basic Biology Laboratory Techniques

Directory of Open Access Journals (Sweden)

Grace A. Maldarelli

2009-12-01

Full Text Available Biology laboratory classes are designed to teach concepts and techniques through experiential learning. Students who have never performed a technique must be guided through the process, which is often difficult to standardize across multiple lab sections. Visual demonstration of laboratory procedures is a key element in teaching pedagogy. The main goals of the study were to create videos explaining and demonstrating a variety of lab techniques that would serve as teaching tools for undergraduate and graduate lab courses and to assess the impact of these videos on student learning. Demonstrations of individual laboratory procedures were videotaped and then edited with iMovie. Narration for the videos was edited with Audacity. Undergraduate students were surveyed anonymously prior to and following screening to assess the impact of the videos on student lab performance by completion of two Participant Perception Indicator surveys. A total of 203 and 171 students completed the pre- and posttesting surveys, respectively. Statistical analyses were performed to compare student perceptions of knowledge of, confidence in, and experience with the lab techniques before and after viewing the videos. Eleven demonstrations were recorded. Chi-square analysis revealed a significant increase in the number of students reporting increased knowledge of, confidence in, and experience with the lab techniques after viewing the videos. Incorporation of instructional videos as prelaboratory exercises has the potential to standardize techniques and to promote successful experimental outcomes.

The Multisensory Sound Lab: Sounds You Can See and Feel.

Science.gov (United States)

Lederman, Norman; Hendricks, Paula

1994-01-01

A multisensory sound lab has been developed at the Model Secondary School for the Deaf (District of Columbia). A special floor allows vibrations to be felt, and a spectrum analyzer displays frequencies and harmonics visually. The lab is used for science education, auditory training, speech therapy, music and dance instruction, and relaxation…

A Multi-Purpose Math Lab: A Place for All Seasons.

Science.gov (United States)

Habib, Berthe

Two services offered by Bellevue Community College's Multipurpose Math Lab are described in this report. The first of these is the lab's drop-in tutorial center, which offers tutorial support, worksheets, and other instructional materials to students at all levels of mathematics course work. This section of the report describes the philosophy of…

SuperFormLab: showing SuperFormLab

DEFF Research Database (Denmark)

2013-01-01

bachelor program, followed by two years of master studies. The courses are offered equally to students from other design disciplines, e.g. industrial design. Teaching is mainly in English as the program is attended by a relatively large group of non-Danish students, who seek exactly this combination......3D-printing in clay and ceramic objects shaped by your own sounds and movements! Digital form transferred via CNC-milling to ornamental ceramic wall-cladding. Brave New World… Students and their teacher at SuperFormLab, the new ceramic workshop of the School of Design at the Royal Danish Academy...... of Fine Arts in Copenhagen, will be showing results of their investigations into the potential of combining digital technologies with ceramic materials. It is now possible to shape the most complex mathematical, virtual 3D objects through the use of advanced software-programs. And more than that – you can...

Providing Learning Computing Labs using Hosting and Virtualization Technologies

Directory of Open Access Journals (Sweden)

Armide González

2011-05-01

Full Text Available This paper presents a computing hosting system to provide virtual computing laboratories for learning activities. This system is based on hosting and virtualization technologies. All the components used in its development are free software tools. The computing lab model provided by the system is a more sustainable and scalable alternative than the traditional academic computing lab, and it requires lower costs of installation and operation.

Guidelines for Urban Labs

DEFF Research Database (Denmark)

Scholl, Christian; Agger Eriksen, Mette; Baerten, Nik

2017-01-01

These guidelines are intended for team members and managers of urban labs and, more generally, for civil servants and facilitators in cities working with experimental processes to tackle complex challenges. They aim to support the everyday practice of collaboratively experimenting and learning ho...... the result is inspiring and instructive for all those who want to wrap their minds around experimental co-creative approaches to urban governance and city development....

Encouraging entrepreneurship in university labs: Research activities, research outputs, and early doctorate careers.

Science.gov (United States)

Roach, Michael

2017-01-01

This paper investigates how the encouragement of entrepreneurship within university research labs relates with research activities, research outputs, and early doctorate careers. Utilizing a panel survey of 6,840 science & engineering doctoral students at 39 R1 research universities, this study shows that entrepreneurship is widely encouraged across university research labs, ranging from 54% in biomedical engineering to 18% in particle physics, while only a small share of labs openly discourage entrepreneurship, from approximately 3% in engineering to approximately 12% in the life sciences. Within fields, there is no difference between labs that encourage entrepreneurship and those that do not with respect to basic research activity and the number of publications. At the same time, labs that encourage entrepreneurship are significantly more likely to report invention disclosures, particularly in engineering where such labs are 41% more likely to disclose inventions. With respect to career pathways, PhDs students in labs that encourage entrepreneurship do not differ from other PhDs in their interest in academic careers, but they are 87% more likely to be interested in careers in entrepreneurship and 44% more likely to work in a startup after graduation. These results persist even when accounting for individuals' pre-PhD interest in entrepreneurship and the encouragement of other non-academic industry careers.

Encouraging entrepreneurship in university labs: Research activities, research outputs, and early doctorate careers

Science.gov (United States)

2017-01-01

This paper investigates how the encouragement of entrepreneurship within university research labs relates with research activities, research outputs, and early doctorate careers. Utilizing a panel survey of 6,840 science & engineering doctoral students at 39 R1 research universities, this study shows that entrepreneurship is widely encouraged across university research labs, ranging from 54% in biomedical engineering to 18% in particle physics, while only a small share of labs openly discourage entrepreneurship, from approximately 3% in engineering to approximately 12% in the life sciences. Within fields, there is no difference between labs that encourage entrepreneurship and those that do not with respect to basic research activity and the number of publications. At the same time, labs that encourage entrepreneurship are significantly more likely to report invention disclosures, particularly in engineering where such labs are 41% more likely to disclose inventions. With respect to career pathways, PhDs students in labs that encourage entrepreneurship do not differ from other PhDs in their interest in academic careers, but they are 87% more likely to be interested in careers in entrepreneurship and 44% more likely to work in a startup after graduation. These results persist even when accounting for individuals’ pre-PhD interest in entrepreneurship and the encouragement of other non-academic industry careers. PMID:28178270

Attracting STEM talent: do STEM students prefer traditional or work/life-interaction labs?

Directory of Open Access Journals (Sweden)

William C DeFraine

Full Text Available The demand for employees trained in science, technology, engineering, and mathematics (STEM fields continues to increase, yet the number of Millennial students pursuing STEM is not keeping pace. We evaluated whether this shortfall is associated with Millennials' preference for flexibility and work/life-interaction in their careers-a preference that may be inconsistent with the traditional idea of a science career endorsed by many lab directors. Two contrasting approaches to running STEM labs and training students were explored, and we created a lab recruitment video depicting each. The work-focused video emphasized the traditional notions of a science lab, characterized by long work hours and a focus on individual achievement and conducting research above all else. In contrast, the work/life-interaction-focused video emphasized a more progressive view - lack of demarcation between work and non-work lives, flexible hours, and group achievement. In Study 1, 40 professors rated the videos, and the results confirmed that the two lab types reflected meaningful real-world differences in training approaches. In Study 2, we recruited 53 current and prospective graduate students in STEM fields who displayed high math-identification and a commitment to science careers. In a between-subjects design, they watched one of the two lab-recruitment videos, and then reported their anticipated sense of belonging to and desire to participate in the lab depicted in the video. Very large effects were observed on both primary measures: Participants who watched the work/life-interaction-focused video reported a greater sense of belonging to (d = 1.49 and desire to participate in (d = 1.33 the lab, relative to participants who watched the work-focused video. These results suggest Millennials possess a strong desire for work/life-interaction, which runs counter to the traditional lab-training model endorsed by many lab directors. We discuss implications of these

Volcanic ash leaching as a means of tracing the environmental impact of the 2011 Grímsvötn eruption, Iceland.

Science.gov (United States)

Cabré, J; Aulinas, M; Rejas, M; Fernandez-Turiel, J L

2016-07-01

The Grímsvötn volcanic eruption, from 21 to 28 May, 2011, was the largest eruption of the Grímsvötn Volcanic System since 1873, with a Volcanic Explosivity Index (VEI) of magnitude 4. The main geochemical features of the potential environmental impact of the volcanic ash-water interaction were determined using two different leaching methods as proxies (batch and vertical flow-through column experiments). Ash consists of glass with minor amounts of plagioclase, clinopyroxene, diopside, olivine and iron sulphide; this latter mineral phase is very rare in juvenile ash. Ash grain morphology and size reflect the intense interaction of magma and water during eruption. Batch and column leaching tests in deionised water indicate that Na, K, Ca, Mg, Si, Cl, S and F had the highest potential geochemical fluxes to the environment. Release of various elements from volcanic ash took place immediately through dissolution of soluble salts from the ash surface. Element solubilities of Grímsvötn ash regarding bulk ash composition were <1 %. Combining the element solubilities and the total estimated mass of tephra (7.29 × 10(14) g), the total inputs of environmentally important elements were estimated to be 8.91 × 10(9) g Ca, 7.02 × 10(9) g S, 1.10 × 10(9) g Cl, 9.91 × 10(8) g Mg, 9.91 × 10(8) g Fe and 1.45 × 10(8) g P The potential environmental problems were mainly associated with the release of F (5.19 × 10(9) g).

LabVIEW: what is it and why is it used?

CERN Multimedia

CERN. Geneva

2017-01-01

LabVIEW is a complicated acronym for Lab Virtual Instrument Engineering Workbench. Something catchy would have been better, because it is a programming language that lets you define your code graphically. From low level data acquisition to high level result presentation, you can perform everything with the same programming method and it makes programming fun.

Indicators for the use of robotic labs in basic biomedical research: a literature analysis

Directory of Open Access Journals (Sweden)

Paul Groth

2017-11-01

Full Text Available Robotic labs, in which experiments are carried out entirely by robots, have the potential to provide a reproducible and transparent foundation for performing basic biomedical laboratory experiments. In this article, we investigate whether these labs could be applicable in current experimental practice. We do this by text mining 1,628 papers for occurrences of methods that are supported by commercial robotic labs. Using two different concept recognition tools, we find that 86%–89% of the papers have at least one of these methods. This and our other results provide indications that robotic labs can serve as the foundation for performing many lab-based experiments.

Removal of eutrophication factors and heavy metal from a closed cultivation system using the macroalgae, Gracilaria sp. (Rhodophyta)

Science.gov (United States)

Kang, Kyoung Ho; Sui, Zhenghong

2010-11-01

In this study, the ability of macroalgae Gracilaria sp. of removing eutrophication factors and toxic heavy metals Al, Cr, and Zn in a closed cultivation system is reported. The results show that the concentration of the three heavy metals decreased significantly during the experimental period in an algal biomass dependent manner. The biofiltration capacity of the alga for Al, Cr, and Zn is 10.1%-72.6%, 52.5%-83.4% and 36.5%-91.7%, respectively. Using more materials resulted in stronger heavy metal removal. Additionally, the concentration of chl- a, TN, TP and DIN of water samples from aquariums involving large, medium, and small algal biomass cultivation increased first and then decreased during the experiment. COD value of all three groups decreased with time and displayed algal biomass dependency: more algae resulting in a greater COD value than those of less biomass. Furthermore, changes in COD reflect an obvious organic particles deprivation process of algae. This is the first report on heavy metal removal effect by Gracilaria species. The results suggest that macroalgae can be used as a biofilter for the treatment of nutrient-enriched or heavy-metal polluted water, to which an appropriate time range should be carefully determined.

DOE EiR at Oakridge National Lab 2008/09

Energy Technology Data Exchange (ETDEWEB)

Bauer, Michael

2012-11-30

This project placed an experienced technology entrepreneur at Oak Ridge National Lab, one of DOE's premier laboratories undertaking cutting edge research in a variety of fields, including energy technologies. With the goal of accelerating the commercialization of advanced energy technologies, the task was to review available technologies at the lab and identify those that qualify for licensing and commercialization by a private startup company, backed by private venture capital. During the project, more than 1,500 inventions filed at the lab were reviewed over a 1 year period; a successively smaller number was selected for more detailed review, ultimately resulting in five, and then 1 technology, being reviewed for immediate commercialization. The chosen technology, consisting in computational chemistry based approached to optimization of enzymes, was tested in lab experiments, paid for by funds raised by ORNL for the purpose of proving out the effectiveness of the technology and readiness for commercialization. The experiments proved out that the technology worked however it's performance proved not yet mature enough to qualify for private venture capital funded commercialization in a high tech startup. As a consequence, the project did not result in a new startup company being formed, as originally intended.

KNMI DataLab experiences in serving data-driven innovations

Science.gov (United States)

Noteboom, Jan Willem; Sluiter, Raymond

2016-04-01

Climate change research and innovations in weather forecasting rely more and more on (Big) data. Besides increasing data from traditional sources (such as observation networks, radars and satellites), the use of open data, crowd sourced data and the Internet of Things (IoT) is emerging. To deploy these sources of data optimally in our services and products, KNMI has established a DataLab to serve data-driven innovations in collaboration with public and private sector partners. Big data management, data integration, data analytics including machine learning and data visualization techniques are playing an important role in the DataLab. Cross-domain data-driven innovations that arise from public-private collaborative projects and research programmes can be explored, experimented and/or piloted by the KNMI DataLab. Furthermore, advice can be requested on (Big) data techniques and data sources. In support of collaborative (Big) data science activities, scalable environments are offered with facilities for data integration, data analysis and visualization. In addition, Data Science expertise is provided directly or from a pool of internal and external experts. At the EGU conference, gained experiences and best practices are presented in operating the KNMI DataLab to serve data-driven innovations for weather and climate applications optimally.

Effect of the polymer concentration on the ON/OFF states of a TN-LCD: polyvinyl alcohol vs. soy lecithin

Science.gov (United States)

de Coss Martinez, Romeo; Gonzalez Murguia, Jose Luis

2011-03-01

In this work we study the response of a Twisted Nematic Liquid Crystal Display (TN-LCD) by varying both the concentration and the polymer used for the microgroove. We compare the performance of two polymers: polyvinyl alcohol and soy lecithin. In particular, the light transmission for the ON/OFF states is evaluated. The polyvinyl alcohol is a polymer widely used in LCDs while lecithin soy is a natural polymer.

Microspectroscopy At Beamline 73 MAX-lab

International Nuclear Information System (INIS)

Engdahl, Anders

2010-01-01

Presentation of some projects at the infrared microspectroscopy experimental station at beamline 73 MAX-lab. Among the subjects are found identification of organic residues in fossil material and examination of the chemistry in an old oak wood wreck.

Simulation and visualization of fundamental optics phenomenon by LabVIEW

Science.gov (United States)

Lyu, Bohan

2017-08-01

Most instructors teach complex phenomenon by equation and static illustration without interactive multimedia. Students usually memorize phenomenon by taking note. However, only note or complex formula can not make user visualize the phenomenon of the photonics system. LabVIEW is a good tool for in automatic measurement. However, the simplicity of coding in LabVIEW makes it not only suit for automatic measurement, but also suitable for simulation and visualization of fundamental optics phenomenon. In this paper, five simple optics phenomenon will be discuss and simulation with LabVIEW. They are Snell's Law, Hermite-Gaussian beam transverse mode, square and circular aperture diffraction, polarization wave and Poincare sphere, and finally Fabry-Perrot etalon in spectrum domain.

In vivo study of the HC-TN strain of hepatitis C virus recovered from a patient with fulminant hepatitis: RNA transcripts of a molecular clone (pHC-TN) are infectious in chimpanzees but not in Huh7.5 cells

DEFF Research Database (Denmark)

Sakai, Akito; Takikawa, Shingo; Thimme, Robert

2007-01-01

Both viral and host factors are thought to influence the pathogenesis of hepatitis C virus (HCV) infection. We studied strain HC-TN (genotype 1a), which caused fulminant hepatic failure in a patient and, subsequently, severe hepatitis in a chimpanzee (CH1422), to analyze the relationship between...... persistently infected. CH1579 and CH1581, despite their differing outcomes, both developed significant intrahepatic cellular immune responses, but not antibodies to the envelope glycoproteins or neutralizing antibodies, during the acute infection. We analyzed the polyprotein sequences of virus recovered...

Design Lab 2005 : pilk steriilsesse elektrotulevikku

Index Scriptorium Estoniae

2005-01-01

Design Lab kutsub disainereid ja üliõpilasi üle terve maailma tegelema kaugemale tulevikku suunatud visioonidega. 2005. a. konkurss otsis nutikaid ja säästlikke lahendusi, mis võiksid 2020. a. kodudes olla juba juurdunud, keskenduti kodutehnikale

Releasing characteristics and fate of heavy metals from phytoremediation crop residues during anaerobic digestion.

Science.gov (United States)

Lee, Jongkeun; Park, Ki Young; Cho, Jinwoo; Kim, Jae Young

2018-01-01

In this study, lab-scale batch tests were conducted to investigate releasing characteristics of heavy metals according to degradation of heavy metal containing biomass. The fate of heavy metals after released from biomass was also determined through adsorption tests and Visual MINTEQ simulation. According to the anaerobic batch test results as well as volatile solids and carbon balance analyses, maximum of 60% by wt. of biomass was degraded. During the anaerobic biodegradation, among Cd, Cu, Ni, Pb, and Zn, only Cu and Zn were observed in soluble form (approximately 40% by wt. of input mass). The discrepancy between degradation ratio of biomass and ratio of released heavy metals mass from biomass was observed. It seems that this discordance was caused by the fate (i.e., precipitated with sulfur/hydroxide or adsorbed onto sorbents) of each heavy metal types in solution after being released from biomass. Thus, releasing characteristics and fate of heavy metal should be considered carefully to predict stability of anaerobic digestion process for heavy metal-containing biomass. Copyright © 2017 Elsevier Ltd. All rights reserved.

Using collaborative technologies in remote lab delivery systems for topics in automation

Science.gov (United States)

Ashby, Joe E.

Lab exercises are a pedagogically essential component of engineering and technology education. Distance education remote labs are being developed which enable students to access lab facilities via the Internet. Collaboration, students working in teams, enhances learning activity through the development of communication skills, sharing observations and problem solving. Web meeting communication tools are currently used in remote labs. The problem identified for investigation was that no standards of practice or paradigms exist to guide remote lab designers in the selection of collaboration tools that best support learning achievement. The goal of this work was to add to the body of knowledge involving the selection and use of remote lab collaboration tools. Experimental research was conducted where the participants were randomly assigned to three communication treatments and learning achievement was measured via assessments at the completion of each of six remote lab based lessons. Quantitative instruments used for assessing learning achievement were implemented, along with a survey to correlate user preference with collaboration treatments. A total of 53 undergraduate technology students worked in two-person teams, where each team was assigned one of the treatments, namely (a) text messaging chat, (b) voice chat, or (c) webcam video with voice chat. Each had little experience with the subject matter involving automation, but possessed the necessary technical background. Analysis of the assessment score data included mean and standard deviation, confirmation of the homogeneity of variance, a one-way ANOVA test and post hoc comparisons. The quantitative and qualitative data indicated that text messaging chat negatively impacted learning achievement and that text messaging chat was not preferred. The data also suggested that the subjects were equally divided on preference to voice chat verses webcam video with voice chat. To the end of designing collaborative

Encouraging entrepreneurship in university labs: Research activities, research outputs, and early doctorate careers.

Directory of Open Access Journals (Sweden)

Michael Roach

Full Text Available This paper investigates how the encouragement of entrepreneurship within university research labs relates with research activities, research outputs, and early doctorate careers. Utilizing a panel survey of 6,840 science & engineering doctoral students at 39 R1 research universities, this study shows that entrepreneurship is widely encouraged across university research labs, ranging from 54% in biomedical engineering to 18% in particle physics, while only a small share of labs openly discourage entrepreneurship, from approximately 3% in engineering to approximately 12% in the life sciences. Within fields, there is no difference between labs that encourage entrepreneurship and those that do not with respect to basic research activity and the number of publications. At the same time, labs that encourage entrepreneurship are significantly more likely to report invention disclosures, particularly in engineering where such labs are 41% more likely to disclose inventions. With respect to career pathways, PhDs students in labs that encourage entrepreneurship do not differ from other PhDs in their interest in academic careers, but they are 87% more likely to be interested in careers in entrepreneurship and 44% more likely to work in a startup after graduation. These results persist even when accounting for individuals' pre-PhD interest in entrepreneurship and the encouragement of other non-academic industry careers.

The effect of Sm-doping on optical properties of LaB6 nanoparticles

International Nuclear Information System (INIS)

Chao, Luomeng; Bao, Lihong; Shi, Junjie; Wei, Wei; Tegus, O.; Zhang, Zhidong

2015-01-01

Highlights: • Nanoparticles of Sm-doped LaB 6 have been prepared by solid state reaction. • All samples exhibit high absorbance in NIR range and UV range. • The increase of Sm-doping amount shifts the position of minimum absorptance value. • The optical properties of Sm-doped LaB 6 were interpreted by DFT theory. - Abstract: Nanocrystalline particles of LaB 6 , SmB 6 and Sm-doped LaB 6 have been prepared by a solid-state reaction in order to investigate the optical properties of ternary rare-earth hexaborides. The sizes of prepared nanoparticles range from dozens to more than 200 nm, as confirmed by XRD, SEM and TEM examinations. The optical property concerning the absorption spectra was tested with ultraviolet-visible-near infrared (UV-vis-NIR) absorption spectrum. All samples exhibit high absorbance in NIR range and UV range. The increase of Sm-doping amount shifts the position of minimum absorptance value of LaB 6 to the long-wave direction. Density functional theory (DFT) is employed to interpret the optical properties of Sm-doped LaB 6 , and results indicate that Sm 4f states change the DOS at near Fermi surface of LaB 6 after Sm doping and the reduced number of conduction electrons results into the change of absorption spectra

[Leaching characteristics of heavy metals and utilization of filter media in BAF].

Science.gov (United States)

Zou, Jin-long; Dai, Ying

2007-10-01

A series of leaching tests were conducted to study the solidification of heavy metals in biological filter media made with dried sludge as an additive. The maximum leaching contents of Cd, Cr, Cu and Pb are obtained when pH is 1; leaching contents of heavy metals have an obvious decrease as pH is greater than or equal to 3; and it can be concluded from the results that pH has a significant influence on the leaching characteristic of heavy metals at leaching time of either 24 h or 30 d. X-ray diffraction analysis performed on filter media reveal the main compounds of the 4 heavy metals are Pb2O(CrO4), CdSiO3 and CuO, and the heavy metals are solidified in the mesh structure of Si--O. Heavy metals (such as Cd, Cr, Cu and Pb) can be solidified in filter media through a series of crystalline phase changes and chemical reaction after high temperature sintering. The new filter media (obtained in test) were used in biological aerated filter (BAF) to treat wastewater (C/N about 11.5 and 25.5) in a simultaneous nitrification and denitrification (SND) system. Based on the mechanism of SND, the average removal efficienciesof NH4(+)-N and TN filled with the new filter media (obtained in test) are about 85.5%, 90.3%, 46.6% and 49.6%, respectively, and it is higher than those of other 3 medias (Jiangxi ceramsite, Guangzhou ceramsite and Shanxi activated carbon). The results provide a better understanding of factors that may affect the immobilization and leaching characteristics of heavy metals in ceramsite, which promotes the extensive use of filter media in BAF.

Enhancing pre-service physics teachers' creative thinking skills through HOT lab design

Science.gov (United States)

Malik, Adam; Setiawan, Agus; Suhandi, Andi; Permanasari, Anna

2017-08-01

A research on the implementation of HOT (Higher Order Thinking) Laboratory has been carried out. This research is aimed to compare increasing of creative thinking skills of pre-service physics teachers who receive physics lesson with HOT Lab and with verification lab for the topic of electric circuit. This research used a quasi-experiment methods with control group pretest-posttest design. The subject of the research is 40 Physics Education pre-service physics teachers of UIN Sunan Gunung Djati Bandung. Research samples were selected by class random sampling technique. Data on pre-service physics teachers' creative thinking skills were collected using test of creative thinking skills in the form of essay. The results of the research reveal that average of N-gain of creative thinking skills are for pre-service physics teachers who received lesson with HOT Lab design and for pre-service physics teachers who received lesson with verification lab, respectively. Therefore, we conclude that application of HOT Lab design is more effective to increase creative thinking skills in the lesson of electric circuit.

A Living Lab as a Service: Creating Value for Micro-enterprises through Collaboration and Innovation

Directory of Open Access Journals (Sweden)

Anna Ståhlbröst

2013-11-01

Full Text Available The need to innovate is increasingly important for all types and sizes of organizations, but the opportunities for innovation differ substantially between them. For micro-, small,- and medium-sized enterprises, innovation activities are both crucial and demanding because of limited resources, competencies, or vision to innovate their offerings. To support these organizations, the concept of living labs as a service has started to emerge. This concept refers to living labs offering services such as designing the idea-generation processes, planning or carrying out real-world tests of innovations, and pre-market launch assessments. In this article, we will present the findings from a study of micro-enterprises operating in the information technology development sector, including the experienced value of services provided to the companies by a research-based living lab. We share experiences from Botnia, our own living lab in northern Sweden. In this living lab, our aim of creating value for customers is of key importance. Our study shows that using a living lab as a service can generate three different types of value: improved innovations, the role the living lab can play, and the support the living lab offers.

Microgrid central controller development and hierarchical control implemetation in the intelligent microgrid lab of Aalborg University

OpenAIRE

Meng, Lexuan; Savaghebi, Mehdi; Andrade, Fabio; Vasquez Quintero, Juan Carlos; Guerrero, Josep M.; Graells Sobré, Moisès

2015-01-01

This paper presents the development of a microgrid central controller in an inverter-based intelligent microgrid (iMG) lab in Aalborg University, Denmark. The iMG lab aims to provide a flexible experimental platform for comprehensive studies of microgrids. The complete control system applied in this lab is based on the hierarchical control scheme for microgrids and includes primary, secondary and tertiary control. The structure of the lab, including the lab facilities, configurations and comm...

Interesting Guided-Inquiry Labs for a Large-Enrollment, Active Learning Physics II Course

Science.gov (United States)

Wagoner, Kasey; Hynes, K. Mairin; Flanagan, Daniel

2018-04-01

Introductory physics labs often focus on a series of common experiments intending to teach the student the measurement side of physics. While these experiments have the potential to be quite instructive, we observed that our students often consider them to be boring and monotonous, which often leads to them being uninstructive. To combat this, we have designed a series of labs with two major goals: the experiments should be relevant to the students' world, and the labs should gently guide the students to develop the experimental process on their own. Meeting these goals is difficult, particularly in a course with large enrollment where labs are instructed by graduate students. We have had success meeting these goals in our classroom, where over the last decade our introductory physics course has transformed from a traditional, lecture-learning class to a flipped class based on the textbook Six Ideas that Shaped Physics. Here we describe the structure of the new labs we have designed to capitalize on our classroom success while overcoming the aforementioned difficulties. These new labs are more engaging and instructive for our introductory physics students.

Applying a Living Lab methodology to support innovation in education at a university in South Africa

Directory of Open Access Journals (Sweden)

Ronel Callaghan

2015-07-01

Full Text Available The Living Lab paradigm creates open and inter-disciplinary environments where participants can interrogate challenges and co-create solutions. A successful Living Lab context incorporates a clear focus/vision, strong leadership, self-sustainability, a strong sense of community-owned challenges and the potential for sustainable community development. This paper discusses and outlines the elements of Living Labs, and how these have played a role in the establishment of a new Education Living Lab at a University in South Africa. Core values, stakeholders and key success factors of Living Labs are discussed. This is followed by the description of a case study of the establishment process of a Living Lab. The newly established Living Lab already shows success with collaborations and innovation between communities, industry, academia, learners and schools. This is illustrated in an application of the discussions on the Mobile Learning focus area - the first active sub-focus area within the Education Living Lab.

Two types of phytases (histidine acid phytase and β-propeller phytase) in Serratia sp. TN49 from the gut of Batocera horsfieldi (coleoptera) larvae.

Science.gov (United States)

Zhang, Rui; Yang, Peilong; Huang, Huoqing; Shi, Pengjun; Yuan, Tiezheng; Yao, Bin

2011-11-01

Microbial phytases play a major role in the mineralization of organic phosphorous, especially in symbiotic plants and animals. In this study, we identified two types of phytases in Serratia sp. TN49 that was harbored in the gut of Batocera horsfieldi (Coleoptera) larvae. The two phytases, an acidic histidine acid phosphatase (PhyH49) and an alkaline β-propeller phytase (PhyB49), shared low identities with known phytases (61% at most). PhyH49 and PhyB49 produced in Escherichia coli exhibited maximal activities at pH 5.0 (60°C) and pH 7.5-8.0 (45°C), respectively, and are complementary in phytate degradation over the pH range 2.0-9.0. Serratia sp. TN49 harboring both PhyH49 and PhyB49 might make it more adaptive to environment change, corresponding to the evolution trend of microorganism.

Effects of Implementing a Hybrid Wet Lab and Online Module Lab Curriculum into a General Chemistry Course: Impacts on Student Performance and Engagement with the Chemistry Triplet

Science.gov (United States)

Irby, Stefan M.; Borda, Emily J.; Haupt, Justin

2018-01-01

Here, we describe the implementation a hybrid general chemistry teaching laboratory curriculum that replaces a portion of a course's traditional "wet lab" experiences with online virtual lab modules. These modules intentionally utilize representations on all three levels of the chemistry triplet-macroscopic, submicroscopic, and symbolic.…

Isolation and characterization of Tn917lac-generated competence mutants of Bacillus subtilis

International Nuclear Information System (INIS)

Hahn, J.; Albano, M.; Dubnau, D.

1987-01-01

The authors isolated 28 mutants of Bacillus subtilis deficient in the development of competence by using the transposon Tn917lacZ as a mutagen. The mutant strains were poorly transformable with plasmid and chromosomal DNAs but were normally transducible and exhibited wild-type resistance to DNA-damaging agents. The mutations were genetically mapped, and the mutants were characterized with respect to their abilities to bind and take up radiolabeled DNA. All were defective in uptake, and some failed to bind significantly amounts of DNA. The abilities of the mutant strains to resolve into two buoyant density classes on Renografin gradients were studied. Most resolved normally, but several banded in Renografin only at the buoyant density of noncompetent cells. The genetic mapping studies and the other analyses suggested that the mutations define a minimum of seven distinct com genes

Discrimination between Native and Tn6010-Associated oqxAB in Klebsiella spp., Raoultella spp., and other Enterobacteriaceae by Using a Two-Step Strategy

DEFF Research Database (Denmark)

Guillard, Thomas; Lebreil, Anne-Laure; Hansen, Lars Hestbjerg

2015-01-01

ABSTRACT We developed a two-step PCR-based strategy to detect genes encoding OqxAB, allowing a specific assignment of Tn6010-associated oqxAB in Enterobacteriaceae. Chromosomal location in this setup was confirmed by hybridization with I-CeuI-restricted genomes. This approach led us to find...

The Participatory Design of a (Today and) Future Digital Entomology Lab

Science.gov (United States)

Hai-Jew, Shalin

2011-01-01

This article showcases a virtual interactive participatory design activity for building a digital entomology lab. Conceptualized as a virtual complement to a general entomology course at Kansas State University, the lab would allow learners to explore morphological aspects of insects--their various forms and functions--in order to understand…

Using Saccharomyces cerevisiae to Test the Mutagenicity of Household Compounds: An Open Ended Hypothesis-Driven Teaching Lab

OpenAIRE

Marshall, Pamela A.

2007-01-01

In our Fundamentals of Genetics lab, students perform a wide variety of labs to reinforce and extend the topics covered in lecture. I developed an active-learning lab to augment the lecture topic of mutagenesis. In this lab exercise, students determine if a compound they bring from home is a mutagen. Students are required to read extensive background material, perform research to find a potential mutagen to test, develop a hypothesis, and bring to the lab their own suspected mutagen. This lab...

Loading and transport of high-active waste (HAW) with the TN85 flask in 2008

International Nuclear Information System (INIS)

Rys, Michael; Horn, Thomas; Graf, Wilhelm; Bonface, Jean-Michael

2009-01-01

As a part of the operation of nuclear power plants, it is essential to safely manage the radioactive waste. With new developments in science and technology, it is a dynamic process to adapt procedures, equipment and flasks to be used in the future. This is a task for specialists - a task for GNS Gesellschaft fuer Nuklear-Service mbH and for TN International. Until 1994 reprocessing of spent fuel from German nuclear power plants was mandatory for the Utilities (EVU) in Germany. Basis for the reprocessing was the German Atomic Act. The German Utilities concluded contracts on reprocessing with Compagnie Generale des Matieres Nucleaires (COGEMA, now AREVA NC) in France and British Nuclear Fuels plc (BNFL, now INS) in England. The total amount to be reprocessed comes to 5309 t HM contracted to AREVA NC and 768 t HM contracted to INS. The waste generated from reprocessing - or an equivalent amount of radioactive material - has to be returned to the country of origin. In 1979 already an exchange of notes took place between the German and the French government with the obligation of both sides to enable and support the return of reprocessing residues or equivalents. The return of high-active waste (HAW) from France has started in 1996 with the first attribution of 28 glass canisters (one flask) to German Utilities by AREVA NC. Until 2007, 75 flasks loaded with vitrified residue (VR) canisters have been transported to Gorleben. For these transports CASTOR registered HAW 20/28 CG flasks have been used. This presentation will give some background information about the last HAW transport in 2008 with the new flask generation of the type TN85. It will also describe the assembly of the new flask, the preparation of the flask for the loading campaign as well as the loading procedure. (orig.)

Loading and transport of high-active waste (HAW) with the TN85 flask in 2008

Energy Technology Data Exchange (ETDEWEB)

Rys, Michael; Horn, Thomas; Graf, Wilhelm [GNS Gesellschaft fuer Nuklear-Service mbH (Germany); Bonface, Jean-Michael [TN International, Montigny-le-Bretonneux (France)

2009-07-01

As a part of the operation of nuclear power plants, it is essential to safely manage the radioactive waste. With new developments in science and technology, it is a dynamic process to adapt procedures, equipment and flasks to be used in the future. This is a task for specialists - a task for GNS Gesellschaft fuer Nuklear-Service mbH and for TN International. Until 1994 reprocessing of spent fuel from German nuclear power plants was mandatory for the Utilities (EVU) in Germany. Basis for the reprocessing was the German Atomic Act. The German Utilities concluded contracts on reprocessing with Compagnie Generale des Matieres Nucleaires (COGEMA, now AREVA NC) in France and British Nuclear Fuels plc (BNFL, now INS) in England. The total amount to be reprocessed comes to 5309 t HM contracted to AREVA NC and 768 t HM contracted to INS. The waste generated from reprocessing - or an equivalent amount of radioactive material - has to be returned to the country of origin. In 1979 already an exchange of notes took place between the German and the French government with the obligation of both sides to enable and support the return of reprocessing residues or equivalents. The return of high-active waste (HAW) from France has started in 1996 with the first attribution of 28 glass canisters (one flask) to German Utilities by AREVA NC. Until 2007, 75 flasks loaded with vitrified residue (VR) canisters have been transported to Gorleben. For these transports CASTOR {sup registered} HAW 20/28 CG flasks have been used. This presentation will give some background information about the last HAW transport in 2008 with the new flask generation of the type TN85. It will also describe the assembly of the new flask, the preparation of the flask for the loading campaign as well as the loading procedure. (orig.)

SciLab Based Remote Control of Thermo-Optical Plant

Directory of Open Access Journals (Sweden)

Miroslav Jano

2011-11-01

Full Text Available The paper deals with the web-based implementation of the control system of a thermo-optical plant. The control of the plant is based on the SciLab software which originally is not designed for web-based applications. The paper shows a possible way to circumvent this limitation. The ultimate goal is to enable remote controlled experiment using SciLab. The paper also describes possible tools for communication and control of the real plant and visualization of results.

Integrated lasers for polymer Lab-on-a-Chip systems

DEFF Research Database (Denmark)

Mappes, Timo; Vannahme, Christoph; Grosmann, Tobias

2012-01-01

We develop optical Lab-on-a-Chips on different platforms for marker-based and label-free biophotonic sensor applications. Our chips are based on polymers and fabricated by mass production technologies to integrate microfluidic channels, optical waveguides and miniaturized lasers.......We develop optical Lab-on-a-Chips on different platforms for marker-based and label-free biophotonic sensor applications. Our chips are based on polymers and fabricated by mass production technologies to integrate microfluidic channels, optical waveguides and miniaturized lasers....

Sbírka rukopisných zlomků ve Státním okresním archivu v Kutné Hoře

Czech Academy of Sciences Publication Activity Database

Brodský, Pavel; Hradilová, Marta; Hrdinová, Martina; Petr, Stanislav; Tošnerová, Marie

2013-01-01

Roč. 43, č. 0 (2013), s. 207-248 ISSN 0585-5691 R&D Projects: GA ČR GAP405/10/0027 Institutional support: RVO:67985921 Keywords : codicology * manuscripts fragments * Státní okresní archiv Kutná Hora Subject RIV: AB - History

Statusrapport, Teaching Lab, 2007-2012, Læreruddannelsen i Nørre Nissum

DEFF Research Database (Denmark)

Roswall, Torben Bo

2013-01-01

Rapporten gør rede for det forskningsmæssige grundlag for Teaching Lab, udviklingsfaserne, den praktiske udmøntning, resultater af den løbende evaluering – herunder ”Bridging The Gap”-følgeforskning – samt en spejling af Teaching Lab i resultaterne af den nyeste, internationale forskning i...

Needsfinding and design of a Living lab in the built environment

NARCIS (Netherlands)

Savelkoul, L.E.M.; Peutz, M.

2017-01-01

Involvement of (end) users is essential in Living Labs. Testing assumptions by exhibiting these to users leads to new insights and often also to innovation. In this study, we show these phenomena by use of a Living Lab in a health care organization. We examine user involvement by researching their

FameLab - Swiss Semi Finals

CERN Multimedia

Corinne Pralavorio

2012-01-01

Twenty-two young scientists participated in the FameLab semi-final at CERN's Globe of Science and Innovation on 4 February, supported by a large audience and by more than 100 fans following via webcast. A panel of judges chose Lemmer and four other candidates to join five other semi-finalists at the national finals in Zurich on 30 March.

Encouraging entrepreneurship in university labs: Research activities, research outputs, and early doctorate careers

OpenAIRE

Roach, Michael

2017-01-01

This paper investigates how the encouragement of entrepreneurship within university research labs relates with research activities, research outputs, and early doctorate careers. Utilizing a panel survey of 6,840 science & engineering doctoral students at 39 R1 research universities, this study shows that entrepreneurship is widely encouraged across university research labs, ranging from 54% in biomedical engineering to 18% in particle physics, while only a small share of labs openly discoura...

Microbial Life in a Winogradsky Column: From Lab Course to Diverse Research Experience ?

OpenAIRE

Parks, Samantha T.

2015-01-01

Many traditional lab courses include both standard and inquiry-based experiments, yet lack cooperative and authentic lab experiences.  Such experiences are important for microbiology students and burgeoning researchers.  In a novel lab environment, students constructed Winogradsky columns using common soil and water sources.  During initial column incubation, students learned methods for identification of microbial isolates including staining, microscopy, biochemistry and 16S-rRNA sequencing....

Enhancing Communication Skills of Pre-service Physics Teacher through HOT Lab Related to Electric Circuit

Science.gov (United States)

Malik, A.; Setiawan, A.; Suhandi, A.; Permanasari, A.; Dirgantara, Y.; Yuniarti, H.; Sapriadil, S.; Hermita, N.

2018-01-01

This study aimed to investigate the improvement to pre-service teacher’s communication skills through Higher Order Thinking Laboratory (HOT Lab) on electric circuit topic. This research used the quasi-experiment method with pretest-posttest control group design. Research subjects were 60 students of Physics Education in UIN Sunan Gunung Djati Bandung. The sample was chosen by random sampling technique. Students’ communication skill data collected using a communication skills test instruments-essays form and observations sheets. The results showed that pre-service teacher communication skills using HOT Lab were higher than verification lab. Student’s communication skills in groups using HOT Lab were not influenced by gender. Communication skills could increase due to HOT Lab based on problems solving that can develop communication through hands-on activities. Therefore, the conclusion of this research shows the application of HOT Lab is more effective than the verification lab to improve communication skills of pre-service teachers in electric circuit topic and gender is not related to a person’s communication skills.

ExoGeoLab Pilot Project for Landers, Rovers and Instruments

Science.gov (United States)

Foing, Bernard

2010-05-01

We have developed a pilot facility with a Robotic Test Bench (ExoGeoLab) and a Mobile Lab Habitat (ExoHab). They can be used to validate concepts and external instruments from partner institutes. The ExoGeoLab research incubator project, has started in the frame of a collaboration between ILEWG (International Lunar Exploration working Group http://sci.esa.int/ilewg), ESTEC, NASA and academic partners, supported by a design and control desk in the European Space Incubator (ESI), as well as infrastructure. ExoGeoLab includes a sequence of technology and research pilot project activities: - Data analysis and interpretation of remote sensing and in-situ data, and merging of multi-scale data sets - Procurement and integration of geophysical, geo-chemical and astrobiological breadboard instruments on a surface station and rovers - Integration of cameras, environment and solar sensors, Visible and near IR spectrometer, Raman spectrometer, sample handling, cooperative rovers - Delivery of a generic small planetary lander demonstrator (ExoGeoLab lander, Sept 2009) as a platform for multi-instruments tests - Research operations and exploitation of ExoGeoLab test bench for various conceptual configurations, and support for definition and design of science surface packages (Moon, Mars, NEOs, outer moons) - Field tests of lander, rovers and instruments in analogue sites (Utah MDRS 2009 & 2010, Eifel volcanic park in Sept 2009, and future campaigns). Co-authors, ILEWG ExoGeoLab & ExoHab Team: B.H. Foing(1,11)*#, C. Stoker(2,11)*, P. Ehrenfreund(10,11), L. Boche-Sauvan(1,11)*, L. Wendt(8)*, C. Gross(8, 11)*, C. Thiel(9)*, S. Peters(1,6)*, A. Borst(1,6)*, J. Zavaleta(2)*, P. Sarrazin(2)*, D. Blake(2), J. Page(1,4,11), V. Pletser(5,11)*, E. Monaghan(1)*, P. Mahapatra(1)#, A. Noroozi(3), P. Giannopoulos(1,11) , A. Calzada(1,6,11), R. Walker(7), T. Zegers(1, 15) #, G. Groemer(12)# , W. Stumptner(12)#, B. Foing(2,5), J. K. Blom(3)#, A. Perrin(14)#, M. Mikolajczak(14)#, S. Chevrier(14

NASA GeneLab Project: Bridging Space Radiation Omics with Ground Studies.

Science.gov (United States)

Beheshti, Afshin; Miller, Jack; Kidane, Yared; Berrios, Daniel; Gebre, Samrawit G; Costes, Sylvain V

2018-04-13

Accurate assessment of risks of long-term space missions is critical for human space exploration. It is essential to have a detailed understanding of the biological effects on humans living and working in deep space. Ionizing radiation from galactic cosmic rays (GCR) is a major health risk factor for astronauts on extended missions outside the protective effects of the Earth's magnetic field. Currently, there are gaps in our knowledge of the health risks associated with chronic low-dose, low-dose-rate ionizing radiation, specifically ions associated with high (H) atomic number (Z) and energy (E). The NASA GeneLab project ( https://genelab.nasa.gov/ ) aims to provide a detailed library of omics datasets associated with biological samples exposed to HZE. The GeneLab Data System (GLDS) includes datasets from both spaceflight and ground-based studies, a majority of which involve exposure to ionizing radiation. In addition to detailed information on radiation exposure for ground-based studies, GeneLab is adding detailed, curated dosimetry information for spaceflight experiments. GeneLab is the first comprehensive omics database for space-related research from which an investigator can generate hypotheses to direct future experiments, utilizing both ground and space biological radiation data. The GLDS is continually expanding as omics-related data are generated by the space life sciences community. Here we provide a brief summary of the space radiation-related data available at GeneLab.

Dr. Monaco Examines Lab-on a-Chip

Science.gov (United States)

2003-01-01

Dr. Lisa Monaco, Marshall Space Flight Center's (MSFC's) project scientist for the Lab-on-a-Chip Applications Development (LOCAD) program, examines a lab on a chip. The small dots are actually ports where fluids and chemicals can be mixed or samples can be collected for testing. Tiny channels, only clearly visible under a microscope, form pathways between the ports. Many chemical and biological processes, previously conducted on large pieces of laboratory equipment, can now be performed on these small glass or plastic plates. Monaco and other researchers at MSFC in Huntsville, Alabama, are customizing the chips to be used for many space applications, such as monitoring microbes inside spacecraft and detecting life on other planets. The portable, handheld Lab-on-a Chip Application Development Portable Test System (LOCAD-PTS) made its debut flight aboard Discovery during the STS-116 mission launched December 9, 2006. The system allowed crew members to monitor their environment for problematic contaminants such as yeast, mold, and even E.coli, and salmonella. Once LOCAD-PTS reached the International Space Station (ISS), the Marshall team continued to manage the experiment, monitoring the study from a console in the Payload Operations Center at MSFC. The results of these studies will help NASA researchers refine the technology for future Moon and Mars missions. (NASA/MSFC/D.Stoffer)

Living labs an arena for development and testing Ambient Assisted living technology

DEFF Research Database (Denmark)

Lassen, Anna Marie; Bangshaab, Jette

everyday activities. Conclusion: Based on staff and end user interviews, the study were able to conclude that independence is the main motivation for using AAL-technology. Application to Practice: The results are now used at the municipality level in several areas. The project has provided a more user......Background: This gives an example of Living labs as an arena for development/testing Ambient Assisted Living technology (AAL-technology). The selected Living lab is part of an EU-supported development project in collaboration with practice and concerns a Living lab that has developed...... an implementation model for an AAL-technology – toilets with douche and drying. (2) Method: The study involves Living lab as location for technology development/testing as well as user-driven approaches to obtain initial data. (1) Moreover, the study is based on process interviews, qualitative research interviews...

Frequency spectrum analysis of 252Cf neutron source based on LabVIEW

International Nuclear Information System (INIS)

Mi Deling; Li Pengcheng

2011-01-01

The frequency spectrum analysis of 252 Cf Neutron source is an extremely important method in nuclear stochastic signal processing. Focused on the special '0' and '1' structure of neutron pulse series, this paper proposes a fast-correlation algorithm to improve the computational rate of the spectrum analysis system. And the multi-core processor technology is employed as well as multi-threaded programming techniques of LabVIEW to construct frequency spectrum analysis system of 252 Cf neutron source based on LabVIEW. It not only obtains the auto-correlation and cross correlation results, but also auto-power spectrum,cross-power spectrum and ratio of spectral density. The results show that: analysis tools based on LabVIEW improve the fast auto-correlation and cross correlation code operating efficiency about by 25% to 35%, also verify the feasibility of using LabVIEW for spectrum analysis. (authors)

Baseball Physics: A New Mechanics Lab

Science.gov (United States)

Wagoner, Kasey; Flanagan, Daniel

2018-01-01

The game of baseball provides an interesting laboratory for experimenting with mechanical phenomena (there are many good examples in "The Physics Teacher," available on Professor Alan Nathan's website, and discussed in "Physics of Baseball & Softball"). We have developed a lab, for an introductory-level physics course, that…

Institutional profile: the national Swedish academic drug discovery & development platform at SciLifeLab.

Science.gov (United States)

Arvidsson, Per I; Sandberg, Kristian; Sakariassen, Kjell S

2017-06-01

The Science for Life Laboratory Drug Discovery and Development Platform (SciLifeLab DDD) was established in Stockholm and Uppsala, Sweden, in 2014. It is one of ten platforms of the Swedish national SciLifeLab which support projects run by Swedish academic researchers with large-scale technologies for molecular biosciences with a focus on health and environment. SciLifeLab was created by the coordinated effort of four universities in Stockholm and Uppsala: Stockholm University, Karolinska Institutet, KTH Royal Institute of Technology and Uppsala University, and has recently expanded to other Swedish university locations. The primary goal of the SciLifeLab DDD is to support selected academic discovery and development research projects with tools and resources to discover novel lead therapeutics, either molecules or human antibodies. Intellectual property developed with the help of SciLifeLab DDD is wholly owned by the academic research group. The bulk of SciLifeLab DDD's research and service activities are funded from the Swedish state, with only consumables paid by the academic research group through individual grants.

The target vacuum storage facility at iThemba LABS

Science.gov (United States)

Neveling, R.; Kheswa, N. Y.; Papka, P.

2018-05-01

A number of nuclear physics experiments at iThemba LABS require target foils that consist of specific isotopes of elements which are reactive in air. Not only is it important to prepare these targets in a suitable environment to prevent oxidation, but consideration should also be given to the long term storage and handling facilities of such targets. The target vacuum storage facility at iThemba LABS, as well as additional hardware necessary to transport and install the target foils in the experimental chamber, will be discussed.

Incorporating inquiry and the process of science into introductory astronomy labs at the George Washington University

Science.gov (United States)

Cobb, Bethany E.

2018-01-01

Since 2013, the Physics Department at GWU has used student-centered active learning in the introductory astronomy course “Introduction to the Cosmos.” Class time is spent in groups on questions, math problems, and hands-on activities, with multiple instructors circulating to answer questions and engage with the students. The students have responded positively to this active-learning. Unfortunately, in transitioning to active-learning there was no time to rewrite the labs. Very quickly, the contrast between the dynamic classroom and the traditional labs became apparent. The labs were almost uniformly “cookie-cutter” in that the procedure and analysis were specified step-by-step and there was just one right answer. Students rightly criticized the labs for lacking a clear purpose and including busy-work. Furthermore, this class fulfills the GWU scientific reasoning general education requirement and thus includes learning objectives related to understanding the scientific method, testing hypotheses with data, and considering uncertainty – but the traditional labs did not require these skills. I set out to rejuvenate the lab sequence by writing new inquiry labs based on both topic-specific and scientific reasoning learning objectives. While inquiry labs can be challenging for the students, as they require active thinking and creativity, these labs engage the students more thoroughly in the scientific process. In these new labs, whenever possible, I include real astronomical data and ask the students to use digital tools (SDSS SkyServer, SOHO archive) as if they are real astronomers. To allow students to easily plot, manipulate and analyze data, I built “smart” Excel files using formulas, dropdown menus and macros. The labs are now much more authentic and thought-provoking. Whenever possible, students independently develop questions, hypotheses, and procedures and the scientific method is “scaffolded” over the semester by providing more guidance in the

Remembering the early days of the Met Lab

International Nuclear Information System (INIS)

Katz, J.J.

1990-01-01

The Met Lab was set up by the war-time Manhattan District, US Corp of Engineers to (i) find a system using normal uranium in which a chain reaction would occur; (ii) to show that if such a chain reaction did occur, it would be possible to separate plutonium chemically from the uranium matrix and the fission products formed in the chain reactions; and (iii) to prepare plans for the large-scale production of plutonium. Chemistry Section C-1 of the Met Lab was assigned the responsibility for developing separation methods for plutonium production on the industrial scale. This report describes some aspects of daily life in Section C-1

A Nuclear Scale System Based on LabVIEW

International Nuclear Information System (INIS)

Liu Shixing; Gu Qindong

2009-01-01

Nuclear mass scales measure the weight of materials which absorb and attenuate the nuclear radiation when the low energy γ-ray through it and is a non-contact continuous measurement device with simple structure and reliable operation. LabVIEW as a graphical programming language is a standard data acquisition and instrument control software. Based on the principle of nuclear mass scale measuring system, monitoring software for nuclear scale system is designed using LabVIEW programming environment. Software architecture mainly composed of three basic modules which include the monitoring software, databases and Web services. It achieves measurement data acquisition, status monitoring, and data management and has networking functions. (authors)

Lab Streaming Layer Enabled Myo Data Collection Software User Manual

Science.gov (United States)

2017-06-07

SECURITY CLASSIFICATION OF: 17. LIMITATION OF ABSTRACT UU 18. NUMBER OF PAGES 32 19a. NAME OF RESPONSIBLE PERSON...www.tumsenahopayga.com/ wp-content/uploads/2015/12/Myo- By-Thalmic-Labs-Gesture-Sensor-Controller-Armbad-For-iPhone- Android -Mac-and-PC.jpg...uploads/2015/12/Myo-By-Thalmic-Labs- Gesture-Sensor-Controller-Armbad-For-iPhone- Android -Mac-and-PC.jpg) LSL is an open source system for transmitting

Recent advances in lab-on-a-chip for biosensing applications

DEFF Research Database (Denmark)

Lafleur, Josiane P.; Jönsson, Alexander; Senkbeil, Silja

2016-01-01

The marriage of highly sensitive biosensor designs with the versatility in sample handling and fluidic manipulation offered by lab-on-a-chip systems promises to yield powerful tools for analytical and, in particular, diagnostic applications. The field where these two technologies meet is rapidly...... improvements to existing methods. Recent examples, showing a staggering variety of lab-on-a-chip systems for biosensing applications, are presented, tabularized for overview, and briefly discussed....

Fed-batch CHO cell culture for lab-scale antibody production

DEFF Research Database (Denmark)

Fan, Yuzhou; Ley, Daniel; Andersen, Mikael Rørdam

2017-01-01

Fed-batch culture is the most commonly used upstream process in industry today for recombinant monoclonal antibody production using Chinese hamster ovary cells. Developing and optimizing this process in the lab is crucial for establishing process knowledge, which enable rapid and predictable tech......-transfer to manufacturing scale. In this chapter, we will describe stepwise how to carry out fed-batch CHO cell culture for lab-scale antibody production....

Jefferson Lab, a status report

International Nuclear Information System (INIS)

Dunham, B.M.

1996-01-01

Thomas Jefferson National Accelerator Facility (Jefferson Lab; formerly known as CEBAF), operates a 4 GeV, 200 microA continuous wave (CW) electron accelerator that re-circulates the beam five times through two superconducting 400 MeV linacs. Electrons can be extracted from any of the five recirculation passes and beam can be simultaneously delivered to the three experimental halls. As the commissioning stage nears completion, the accelerator is becoming a fully operational machine. Experiments in Hall C have been underway since November 1995 with beam powers of over 300 kW at various energies. Hall A has received beam for spectrometer commissioning, while Hall B is expected to receive its first beam in the fall of 1996. Accelerator availability of greater than 70% during physics runs and excellent beam quality have contributed to making Jefferson Lab a world class laboratory for accelerator-based electromagnetic nuclear physics. With the high performance of the superconducting RF cavities, machine upgrades to 6 GeV, and eventually 8 to 10 GeV are now in the planning stages. Operational and commissioning details concerning all aspects of the machine will be discussed

Jefferson Lab, a status report

International Nuclear Information System (INIS)

Dunham, B.M.

1996-01-01

Thomas Jefferson National Accelerator Facility (Jefferson Lab; formerly known as CEBAF), operates a 4 GeV, 200 μA continuous wave (CW) electron accelerator that re-circulates the beam five times through two superconducting 400 MeV linacs. Electrons can be extracted from any of the five recirculation passes and beam can be simultaneously delivered to the three experimental halls. As the commissioning stage nears completion, the accelerator is becoming a fully operational machine. Experiments in Hall C have been underway since November 1995 with beam powers of over 300 kW at various energies. Hall A has received beam for spectrometer commissioning, while Hall B is expected to receive its first beam in the fall of 1996. Accelerator availability of greater than 70% during physics runs and excellent beam quality have contributed to making Jefferson Lab a world class laboratory for accelerator-based electromagnetic nuclear physics. With the high performance of the superconducting RF cavities, machine upgrades to 6 GeV, and eventually 8 to 10 GeV are now in the planning stages. Operational and commissioning details concerning all aspects of the machine will be discussed. (author)

Lab-scale hydrogen peroxide data from ECBC

Data.gov (United States)

U.S. Environmental Protection Agency — Data from small lab scale tests conducted at ECBC. It contains efficacy data as well as data on env conditions such as temperature, RH, and hydrogen peroxide vapor...

Low energy nuclear spin excitations in Ho metal investigated by high resolution neutron spectroscopy.

Science.gov (United States)

Chatterji, Tapan; Jalarvo, Niina

2013-04-17

We have investigated the low energy excitations in metallic Ho by high resolution neutron spectroscopy. We found at T = 3 K clear inelastic peaks in the energy loss and energy gain sides, along with the central elastic peak. The energy of this low energy excitation, which is 26.59 ± 0.02 μeV at T = 3 K, decreased continuously and became zero at TN ≈ 130 K. By fitting the data in the temperature range 100-127.5 K with a power law we obtained the power-law exponent β = 0.37 ± 0.02, which agrees with the expected value β = 0.367 for a three-dimensional Heisenberg model. Thus the energy of the low energy excitations can be associated with the order parameter.

ESIP Lab: Supporting Development of Earth Sciences Cyberinfrastructure through Innovation Commons

Science.gov (United States)

Burgess, A. B.; Robinson, E.

2017-12-01

The Earth Science Information Partners (ESIP) is an open, networked community that brings together science, data and information technology practitioners from across sectors. Participation in ESIP is beneficial because it provides an intellectual commons to expose, gather and enhance in-house capabilities in support of an organization's own mandate. Recently, ESIP has begun to explore piloting activities that have worked in the U.S. in other countries as a way to facilitate international collaboration and cross-pollination. The newly formed ESIP Lab realizes the commons concept by providing a virtual place to come up with with new solutions through facilitated ideation, take that idea to a low stakes development environment and potentially fail, but if successful, expose developing technology to domain experts through a technology evaluation process. The Lab does this by supporting and funding solution-oriented projects that have discrete development periods and associated budgets across organizations and agencies. In addition, the Lab provides access to AWS cloud computing resources, travel support, virtual and in-person collaborative platform for distributed groups and exposure to the ESIP community as an expert pool. This cycle of ideation to incubation to evaluation and ultimately adoption or infusion of Earth sciences cyberinfrastructure empowers the scientific community and has spawned a variety of developments like community-led ontology portals, ideas for W3C prov standard improvement and an evaluation framework that pushes technology forward and aides in infusion. The Lab is one of these concepts that could be implemented in other countries and the outputs of the Lab would be shared as a commons and available across traditional borders. This presentation will share the methods and the outcomes of the Lab and seed ideas for adoption internationally.

Domain Adaptation Methods for Improving Lab-to-field Generalization of Cocaine Detection using Wearable ECG

OpenAIRE

Natarajan, Annamalai; Angarita, Gustavo; Gaiser, Edward; Malison, Robert; Ganesan, Deepak; Marlin, Benjamin M.

2016-01-01

Mobile health research on illicit drug use detection typically involves a two-stage study design where data to learn detectors is first collected in lab-based trials, followed by a deployment to subjects in a free-living environment to assess detector performance. While recent work has demonstrated the feasibility of wearable sensors for illicit drug use detection in the lab setting, several key problems can limit lab-to-field generalization performance. For example, lab-based data collection...

UTRaLab – Urban Traffic Research Laboratory

Directory of Open Access Journals (Sweden)

Karsten Kozempel

2017-08-01

Full Text Available The Urban Traffic Research Laboratory (UTRaLab is a research and test track for traffic detection methods and sensors. It is located at the Ernst-Ruska-Ufer, in the southeast of the city of Berlin (Germany. The UTRaLab covers 1 km of a highly-frequented urban road and is connected to a motorway. It is equipped with two gantries with distance of 850 m in between and has several outstations for data collection. The gantries contain many different traffic sensors like inductive loops, cameras, lasers or wireless sensors for traffic data acquisition. Additionally a weather station records environmental data. The UTRaLab’s main purposes are the data collection of traffic data on the one hand and testing newly developed sensors on the other hand.

Exploring problem-based cooperative learning in undergraduate physics labs: student perspectives

Science.gov (United States)

Bergin, S. D.; Murphy, C.; Shuilleabhain, A. Ni

2018-03-01

This study examines the potential of problem-based cooperative learning (PBCL) in expanding undergraduate physics students’ understanding of, and engagement with, the scientific process. Two groups of first-year physics students (n = 180) completed a questionnaire which compared their perceptions of learning science with their engagement in physics labs. One cohort completed a lab based on a PBCL approach, whilst the other completed the same experiment, using a more traditional, manual-based lab. Utilising a participant research approach, the questionnaire was co-constructed by researchers and student advisers from each cohort in order to improve shared meaning between researchers and participants. Analysis of students’ responses suggests that students in the PBCL cohort engaged more in higher-order problem-solving skills and evidenced a deeper understanding of the scientific process than students in the more traditional, manual-based cohort. However, the latter cohort responses placed more emphasis on accuracy and measurement in lab science than the PBCL cohort. The students in the PBCL cohort were also more positively engaged with their learning than their counterparts in the manual led group.

Continuous treatment of heavy metal contaminated clay soils by extraction in stirred tanks and in a countercurrent column

NARCIS (Netherlands)

Tuin, B.J.W.; Tels, M.

1991-01-01

Extn. of metals from 2 contaminated waste site clay soils by 0.1-0.3 N HCl solns. was tested in 3 lab. scale, continuous processes: 2 stirred tank reactors (CSTR' s) in series; a countercurrent sieve-plate column fed with flocculated clay soil materials; and a combination of tank reactor and column.

A "Language Lab" for Architectural Design.

Science.gov (United States)

Mackenzie, Arch; And Others

This paper discusses a "language lab" strategy in which traditional studio learning may be supplemented by language lessons using computer graphics techniques to teach architectural grammar, a body of elements and principles that govern the design of buildings belonging to a particular architectural theory or style. Two methods of…

Measure, Then Show: Grasping Human Evolution Through an Inquiry-Based, Data-driven Hominin Skulls Lab.

Science.gov (United States)

Bayer, Chris N; Luberda, Michael

2016-01-01

Incomprehension and denial of the theory of evolution among high school students has been observed to also occur when teachers are not equipped to deliver a compelling case also for human evolution based on fossil evidence. This paper assesses the outcomes of a novel inquiry-based paleoanthropology lab teaching human evolution to high-school students. The inquiry-based Be a Paleoanthropologist for a Day lab placed a dozen hominin skulls into the hands of high-school students. Upon measuring three variables of human evolution, students explain what they have observed and discuss findings. In the 2013/14 school year, 11 biology classes in 7 schools in the Greater New Orleans area participated in this lab. The interviewed teacher cohort unanimously agreed that the lab featuring hominin skull replicas and stimulating student inquiry was a pedagogically excellent method of delivering the subject of human evolution. First, the lab's learning path of transforming facts to data, information to knowledge, and knowledge to acceptance empowered students to themselves execute part of the science that underpins our understanding of deep time hominin evolution. Second, although challenging, the hands-on format of the lab was accessible to high-school students, most of whom were readily able to engage the lab's scientific process. Third, the lab's exciting and compelling pedagogy unlocked higher order thinking skills, effectively activating the cognitive, psychomotor and affected learning domains as defined in Bloom's taxonomy. Lastly, the lab afforded students a formative experience with a high degree of retention and epistemic depth. Further study is warranted to gauge the degree of these effects.

Exploring the changing learning environment of the gross anatomy lab.

Science.gov (United States)

Hopkins, Robin; Regehr, Glenn; Wilson, Timothy D

2011-07-01

The objective of this study was to assess the impact of virtual models and prosected specimens in the context of the gross anatomy lab. In 2009, student volunteers from an undergraduate anatomy class were randomly assigned to study groups in one of three learning conditions. All groups studied the muscles of mastication and completed identical learning objectives during a 45-minute lab. All groups were provided with two reference atlases. Groups were distinguished by the type of primary tools they were provided: gross prosections, three-dimensional stereoscopic computer model, or both resources. The facilitator kept observational field notes. A prepost multiple-choice knowledge test was administered to evaluate students' learning. No significant effect of the laboratory models was demonstrated between groups on the prepost assessment of knowledge. Recurring observations included students' tendency to revert to individual memorization prior to the posttest, rotation of models to match views in the provided atlas, and dissemination of groups into smaller working units. The use of virtual lab resources seemed to influence the social context and learning environment of the anatomy lab. As computer-based learning methods are implemented and studied, they must be evaluated beyond their impact on knowledge gain to consider the effect technology has on students' social development.

An Overview of Dark Matter Experiments at Jefferson Lab

International Nuclear Information System (INIS)

Boyce, James R

2012-01-01

Dark Matter research at Jefferson Lab started in 2006 with the LIght Pseudoscalar and Scalar Search (LIPSS) collaboration to check the validity of results reported by the PVLAS collaboration. In the intervening years interest in dark matter laboratory experiments has grown at Jefferson Lab. Current research underway or in planning stages probe various mass regions covering 14 orders of magnitude: from 10 −6 eV to 100 MeV. This presentation will be an overview of our dark matter searches, three of which focus on the hypothesized A' gauge boson.

Design of LabVIEW based test system software for MDC electronics

International Nuclear Information System (INIS)

Xue Lin; Huazhong Normal Univ., Wuhan; Huang Guangming; Zhang Hongyu; Jiang Xiaoshan; Sheng Huayi; Zhuang Baoan

2006-01-01

This paper presents the design of Test System Software for MDC Electronics. The highly modular software, developed in LabVIEW and VC ++ 6.0, has been applied in hardware debugging and performance test. LabVIEW and its DLL calling mechanism are introduced briefly. Testing functions of the software, as well as its user interfaces, are described in detail. (authors)

Value added or misattributed? A multi-institution study on the educational benefit of labs for reinforcing physics content

Science.gov (United States)

Holmes, N. G.; Olsen, Jack; Thomas, James L.; Wieman, Carl E.

2017-06-01

Instructional labs are widely seen as a unique, albeit expensive, way to teach scientific content. We measured the effectiveness of introductory lab courses at achieving this educational goal across nine different lab courses at three very different institutions. These institutions and courses encompassed a broad range of student populations and instructional styles. The nine courses studied had two key things in common: the labs aimed to reinforce the content presented in lectures, and the labs were optional. By comparing the performance of students who did and did not take the labs (with careful normalization for selection effects), we found universally and precisely no added value to learning course content from taking the labs as measured by course exam performance. This work should motivate institutions and departments to reexamine the goals and conduct of their lab courses, given their resource-intensive nature. We show why these results make sense when looking at the comparative mental processes of students involved in research and instructional labs, and offer alternative goals and instructional approaches that would make lab courses more educationally valuable.

Teaching Ocean Sciences in the 21st Century Classroom: Lab to Classroom Videoconferencing

Science.gov (United States)

Peach, C. L.; Gerwick, W.; Gerwick, L.; Senise, M.; Jones, C. S.; Malloy, K.; Jones, A.; Trentacoste, E.; Nunnery, J.; Mendibles, T.; Tayco, D.; Justice, L.; Deutscher, R.

2010-12-01

Teaching Ocean Science in the 21st Century Classroom (TOST) is a Center for Ocean Sciences Education Excellence (COSEE CA) initiative aimed at developing and disseminating technology-based instructional strategies, tools and ocean science resources for both formal and informal science education. San Diego Unified School District (SDUSD), Scripps Institution of Oceanography (SIO) and the Lawrence Hall of Science (LHS) have established a proving ground for TOST activities and for development of effective, sustainable solutions for researchers seeking to fulfill NSF and other funding agency broader impact requirements. Lab to Classroom Videoconferencing: Advances in Information and Communications Technology (ICT) are making it easier to connect students and researchers using simple online tools that allow them to interact in novel ways. COSEE CA is experimenting with these tools and approaches to identify effective practices for providing students with insight into the research process and close connections to researchers and their laboratory activities. At the same time researchers, including graduate students, are learning effective communication skills and how to align their presentations to specific classroom needs - all from the comfort of their own lab. The lab to classroom videoconferencing described here is an ongoing partnership between the Gerwick marine biomedical research lab and a group of three life science teachers (7th grade) at Pershing Middle School (SDUSD) that started in 2007. Over the last 5 years, the Pershing science teachers have created an intensive, semester-long unit focused on drug discovery. Capitalizing on the teacher team’s well-developed unit of study and the overlap with leading-edge research at SIO, COSEE CA created the videoconferencing program as a broader impact solution for the lab. The team has refined the program over 3 iterations, experimenting with structuring the activities to most effectively reach the students. In the

Winter Maintenance Wash-Water Heavy Metal Removal Pilot Scale Evaluation

Directory of Open Access Journals (Sweden)

Christopher M. Miller

2016-01-01

Full Text Available To encourage sustainable engineering practices, departments of transportation are interested in reusing winter maintenance truck wash water as part of their brine production and future road application. Traffic-related metals in the wash water, however, could limit this option. The objective of this work was to conduct a pilot scale evaluation of heavy metal (copper, zinc, iron, and lead removal in a filtration unit (maximum flow rate of 45 L/minute containing proprietary (MAR Systems Sorbster® media. Three different trials were conducted and approximately 10,000 L of wash water collected from a winter maintenance facility in Ohio was treated with the pilot unit. Lab studies were also performed on six wash-water samples from multiple facilities to assess particle size removal and estimate settling time as a potential removal mechanism during wash-water storage. Pilot unit total metal removal efficiencies were 79%, 77%, 63%, and 94% for copper, zinc, iron, and lead, respectively. Particle settling calculation estimates for copper and zinc show that 10 hours in storage can also effectively reduce heavy metal concentrations in winter maintenance wash water in excess of 70%. These pilot scale results show promise for reducing heavy metal concentrations to an acceptable level for reuse.

Domain Adaptation Methods for Improving Lab-to-field Generalization of Cocaine Detection using Wearable ECG

Science.gov (United States)

Natarajan, Annamalai; Angarita, Gustavo; Gaiser, Edward; Malison, Robert; Ganesan, Deepak; Marlin, Benjamin M.

2016-01-01

Mobile health research on illicit drug use detection typically involves a two-stage study design where data to learn detectors is first collected in lab-based trials, followed by a deployment to subjects in a free-living environment to assess detector performance. While recent work has demonstrated the feasibility of wearable sensors for illicit drug use detection in the lab setting, several key problems can limit lab-to-field generalization performance. For example, lab-based data collection often has low ecological validity, the ground-truth event labels collected in the lab may not be available at the same level of temporal granularity in the field, and there can be significant variability between subjects. In this paper, we present domain adaptation methods for assessing and mitigating potential sources of performance loss in lab-to-field generalization and apply them to the problem of cocaine use detection from wearable electrocardiogram sensor data. PMID:28090605

Library-Labs-for-Science Literacy Courses.

Science.gov (United States)

Pestel, Beverly C.; Engeldinger, Eugene A.

1992-01-01

Describes two library-lab exercises the authors have incorporated into their college chemistry course. The first exercise introduces students to scientific information and familiarizes them with the tools for accessing it. The second provides a framework for evaluating the reliability of that information and addresses the criteria that should be…

Displacing Media: LCD LAB Artistic Residency

Directory of Open Access Journals (Sweden)

Filipe Pais

2012-12-01

Full Text Available This review refers to an artistic residency which took place at LCD LAB -  CAAA at Guimarães, in March, exploring a strategy for media art called Media Displacement. The text introduces the strategy very briefly and describes the residency's organization, structure, processses and the results produced.

Effects of short-term tocopherol (T) feeding on structure-localized protein tyrosine nitration (pTN) patterns of mitochondrial ATPase following endotoxin (LPS) challenge in beef calves.

Science.gov (United States)

Mitochondrial ATPase/Complex-V (MCV) is an electron transport chain (ETC) component needed for ATP synthesis. The ETC, exquisitely sensitive to proinflammatory mediators (PIM), generates oxynitrogen reactants leading to pTN formation as mitochondrial membrane leakage occurs. Immunohistochemical loca...

Assessing the Impact of a Virtual Lab in an Allied Health Program.

Science.gov (United States)

Kay, Robin; Goulding, Helene; Li, Jia

2018-01-01

Competency-based education in health care requires rigorous standards to ensure professional proficiency. Demonstrating competency in hands-on laboratories calls for effective preparation, knowledge, and experience, all of which can be difficult to achieve using traditional teaching methods. Virtual laboratories are an alternative, cost-effective approach to providing students with sufficient preparatory information. Research on the use of virtual labs in allied health education is limited. The current study investigated the benefits, challenges, and perceived impact of a virtual lab in an allied health program. The sample consisted of 64 students (55 females, 9 males) enrolled in a university medical laboratory science program. A convergent mixed-methods approach (Likert survey, open-ended questions, think-aloud protocol data) revealed that students had positive attitudes towards visual learning, authenticity, learner control, organization, and scaffolding afforded by the virtual lab. Challenges reported included navigational difficulties, an absence of control over content selection, and lack of understanding for certain concepts. Over 90% of students agreed that the virtual lab helped them prepare for hands-on laboratory sessions and that they would use this format of instruction again. Overall, 84% of the students agreed that the virtual lab helped them to achieve greater success in learning.

Integration of the HTC Vive into the medical platform MeVisLab

Science.gov (United States)

Egger, Jan; Gall, Markus; Wallner, Jürgen; de Almeida Germano Boechat, Pedro; Hann, Alexander; Li, Xing; Chen, Xiaojun; Schmalstieg, Dieter

2017-03-01

Virtual Reality (VR) is an immersive technology that replicates an environment via computer-simulated reality. VR gets a lot of attention in computer games but has also great potential in other areas, like the medical domain. Examples are planning, simulations and training of medical interventions, like for facial surgeries where an aesthetic outcome is important. However, importing medical data into VR devices is not trivial, especially when a direct connection and visualization from your own application is needed. Furthermore, most researcher don't build their medical applications from scratch, rather they use platforms, like MeVisLab, Slicer or MITK. The platforms have in common that they integrate and build upon on libraries like ITK and VTK, further providing a more convenient graphical interface to them for the user. In this contribution, we demonstrate the usage of a VR device for medical data under MeVisLab. Therefore, we integrated the OpenVR library into MeVisLab as an own module. This enables the direct and uncomplicated usage of head mounted displays, like the HTC Vive under MeVisLab. Summarized, medical data from other MeVisLab modules can directly be connected per drag-and-drop to our VR module and will be rendered inside the HTC Vive for an immersive inspection.

A LabVIEW®-based software for the control of the AUTORAD platform. A fully automated multisequential flow injection analysis Lab-on-Valve (MSFIA-LOV) system for radiochemical analysis

International Nuclear Information System (INIS)

Barbesi, Donato; Vilas, Victor Vicente; Millet, Sylvain; Sandow, Miguel; Colle, Jean-Yves; Heras, Laura Aldave de las

2017-01-01

A LabVIEW®-based software for the control of the fully automated multi-sequential flow injection analysis Lab-on-Valve (MSFIA-LOV) platform AutoRAD performing radiochemical analysis is described. The analytical platform interfaces an Arduino®-based device triggering multiple detectors providing a flexible and fit for purpose choice of detection systems. The different analytical devices are interfaced to the PC running LabVIEW®VI software using USB and RS232 interfaces, both for sending commands and receiving confirmation or error responses. The AUTORAD platform has been successfully applied for the chemical separation and determination of Sr, an important fission product pertinent to nuclear waste. (author)

A LabVIEW®-based software for the control of the AUTORAD platform: a fully automated multisequential flow injection analysis Lab-on-Valve (MSFIA-LOV) system for radiochemical analysis.