WorldWideScience

Sample records for kinase reporter system

  1. RhoA/Rho-Kinase in the Cardiovascular System.

    Science.gov (United States)

    Shimokawa, Hiroaki; Sunamura, Shinichiro; Satoh, Kimio

    2016-01-22

    Twenty years ago, Rho-kinase was identified as an important downstream effector of the small GTP-binding protein, RhoA. Thereafter, a series of studies demonstrated the important roles of Rho-kinase in the cardiovascular system. The RhoA/Rho-kinase pathway is now widely known to play important roles in many cellular functions, including contraction, motility, proliferation, and apoptosis, and its excessive activity induces oxidative stress and promotes the development of cardiovascular diseases. Furthermore, the important role of Rho-kinase has been demonstrated in the pathogenesis of vasospasm, arteriosclerosis, ischemia/reperfusion injury, hypertension, pulmonary hypertension, and heart failure. Cyclophilin A is secreted by vascular smooth muscle cells and inflammatory cells and activated platelets in a Rho-kinase-dependent manner, playing important roles in a wide range of cardiovascular diseases. Thus, the RhoA/Rho-kinase pathway plays crucial roles under both physiological and pathological conditions and is an important therapeutic target in cardiovascular medicine. Recently, functional differences between ROCK1 and ROCK2 have been reported in vitro. ROCK1 is specifically cleaved by caspase-3, whereas granzyme B cleaves ROCK2. However, limited information is available on the functional differences and interactions between ROCK1 and ROCK2 in the cardiovascular system in vivo. Herein, we will review the recent advances about the importance of RhoA/Rho-kinase in the cardiovascular system. © 2016 American Heart Association, Inc.

  2. Transphosphorylation of E. coli proteins during production of recombinant protein kinases provides a robust system to characterize kinase specificity

    Science.gov (United States)

    Protein kinase specificity is of fundamental importance to pathway regulation and signal transduction. Here, we report a convenient system to monitor the activity and specificity of recombinant protein kinases expressed in E.coli. We apply this to the study of the cytoplasmic domain of the plant rec...

  3. Advances in study of perpes simplex virus type 1-thymidine kinase reporter gene imaging

    International Nuclear Information System (INIS)

    Liu Ying; Lan Xiaoli; Zhang Yongxue

    2007-01-01

    Radionuclide reporter gene imaging is an effect way to provide qualitative and quantitative information for gene therapy. There are three systems of reporter gene including kinase reporter gene. perpes simplex virus type 1-thymidine kinase (HSV1-tk) has perfect physical and chemical characteristic which is suit for imaging as reporter gene. It has been widely investigated and intensively researched. Two substrates of HSV1-tk are purine nucleosite derivant and acyclovir derivant, which can also be used as reporter probes of HSV1-tk. (authors)

  4. Her4 and Her2/neu tyrosine kinase domains dimerize and activate in a reconstituted in vitro system.

    Science.gov (United States)

    Monsey, John; Shen, Wei; Schlesinger, Paul; Bose, Ron

    2010-03-05

    Her4 (ErbB-4) and Her2/neu (ErbB-2) are receptor-tyrosine kinases belonging to the epidermal growth factor receptor (EGFR) family. Crystal structures of EGFR and Her4 kinase domains demonstrate kinase dimerization and activation through an allosteric mechanism. The kinase domains form an asymmetric dimer, where the C-lobe surface of one monomer contacts the N-lobe of the other monomer. EGFR kinase dimerization and activation in vitro was previously reported using a nickel-chelating lipid-liposome system, and we now apply this system to all other members of the EGFR family. Polyhistidine-tagged Her4, Her2/neu, and Her3 kinase domains are bound to these nickel-liposomes and are brought to high local concentration, mimicking what happens to full-length receptors in vivo following ligand binding. Addition of nickel-liposomes to Her4 kinase domain results in 40-fold activation in kinase activity and marked enhancement of C-terminal tail autophosphorylation. Activation of Her4 shows a sigmoidal dependence on kinase concentration, consistent with a cooperative process requiring kinase dimerization. Her2/neu kinase activity is also activated by nickel-liposomes, and is increased further by heterodimerization with Her3 or Her4. The ability of Her3 and Her4 to heterodimerize and activate other family members is studied in vitro. Her3 kinase domain readily activates Her2/neu but is a poor activator of Her4, which differs from the prediction made by the asymmetric dimer model. Mutation of Her3 residues (952)ENI(954) to the corresponding sequence in Her4 enhanced the ability of Her3 to activate Her4, demonstrating that sequence differences on the C-lobe surface influence the heterodimerization and activation of ErbB kinase domains.

  5. 21 CFR 862.1215 - Creatine phosphokinase/creatine kinase or isoenzymes test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Creatine phosphokinase/creatine kinase or... Clinical Chemistry Test Systems § 862.1215 Creatine phosphokinase/creatine kinase or isoenzymes test system. (a) Identification. A creatine phosphokinase/creatine kinase or isoenzymes test system is a device...

  6. Arabidopsis MAP kinase 4 negatively regulates systemic acquired resistance

    DEFF Research Database (Denmark)

    Petersen, M.; Brodersen, P.; Naested, H.

    2000-01-01

    Transposon inactivation of Arabidopsis MAP kinase 4 produced the mpk4 mutant exhibiting constitutive systemic acquired resistance (SAR) including elevated salicylic acid (SA) revels, increased resistance to virulent pathogens, and constitutive pathogenesis-related gene expression shown by Northern...... of NPR1. PDF1.2 and THI2.1 gene induction by jasmonate was blocked in mpk4 expressing NahG, suggesting that MPK4 is required for jasmonic acid-responsive gene expression....

  7. Protein Kinase C Enzymes in the Hematopoietic and Immune Systems.

    Science.gov (United States)

    Altman, Amnon; Kong, Kok-Fai

    2016-05-20

    The protein kinase C (PKC) family, discovered in the late 1970s, is composed of at least 10 serine/threonine kinases, divided into three groups based on their molecular architecture and cofactor requirements. PKC enzymes have been conserved throughout evolution and are expressed in virtually all cell types; they represent critical signal transducers regulating cell activation, differentiation, proliferation, death, and effector functions. PKC family members play important roles in a diverse array of hematopoietic and immune responses. This review covers the discovery and history of this enzyme family, discusses the roles of PKC enzymes in the development and effector functions of major hematopoietic and immune cell types, and points out gaps in our knowledge, which should ignite interest and further exploration, ultimately leading to better understanding of this enzyme family and, above all, its role in the many facets of the immune system.

  8. Bosutinib induced pleural effusions: Case report and review of tyrosine kinase inhibitors induced pulmonary toxicity

    Directory of Open Access Journals (Sweden)

    Natalia I. Moguillansky, MD

    2017-01-01

    Full Text Available Tyrosine kinase inhibitors are known to cause pulmonary complications. We report a case of bosutinib related bilateral pleural effusions in a patient with chronic myeloid leukemia. Characteristics of the pleural fluid are presented. We also discuss other tyrosine kinase inhibitors induced pulmonary toxicities, including pulmonary hypertension and interstitial lung disease.

  9. Microcomputer Assisted Interpretative Reporting of Sequential Creatine Kinase (CK) and Lactate Dehydrogenase (LDH) Isoenzyme Determination

    Science.gov (United States)

    Talamo, Thomas S.; Losos, Frank J.; Mercer, Donald W.

    1984-01-01

    We have developed a microcomputer based system for interpretative reporting of creatine kinase (CK) and lactate dehydrogenase (LDH) isoenzyme studies. Patient demographic data and test results (total CK, CK-MB, LD-1, and LD-2) are entered manually through the keyboard. The test results are compared with normal range values and an interpretative report is generated. This report consists of all pertinent demographic information with a graphic display of up to 12 previous CK and LDH isoenzyme determinations. Diagnostic interpretative statements are printed beneath the graphic display following analysis of previously entered test results. The combination of graphic data display and interpretations based on analysis of up to 12 previous specimens provides useful and accurate information to the cardiologist.

  10. Preliminary validation of varicella zoster virus thymidine kinase as a novel reporter gene for PET

    International Nuclear Information System (INIS)

    Deroose, Christophe M.; Chitneni, Satish K.; Gijsbers, Rik; Vermaelen, Peter; Ibrahimi, Abdelilah; Balzarini, Jan; Baekelandt, Veerle; Verbruggen, Alfons; Nuyts, Johan; Debyser, Zeger; Bormans, Guy M.

    2012-01-01

    Introduction: Imaging of gene expression with positron emission tomography (PET) has emerged as a powerful tool for biomedical research during the last decade. The prototypical herpes simplex virus type 1 thymidine kinase (HSV1-TK) PET reporter gene (PRG) is widely used and many other PRGs have also been validated. We investigated varicella zoster virus thymidine kinase (VZV-tk) as new PRG with radiolabeled bicyclic nucleoside analogues (BCNAs) as PET tracers. Methods: The uptake and washout of four different radiolabeled BCNAs was evaluated in cells expressing VZV-tk after lentiviral vector (LV) transduction and in control cells. Metabolism of the tracers was assayed by high pressure liquid chromatography (HPLC). Mice bearing VZV-TK expressing xenografts were imaged with PET. Results: High uptake in VZV-tk expressing cells was seen for 3 of the 4 tracers tested. The uptake of the tracers could be blocked by the presence of excess thymidine in the incubation solution. Cellular retention was variable, with one tracer showing an acceptable half-life of ∼ 1 hour. The amount of intracellular tracer correlated with the titer of LV used to transduce the cells. VZV-TK dependent conversion into metabolites was shown by HPLC. No specific accumulation was observed in cells expressing a fusion protein containing an HSV1-TK moiety. VZV-tk expression in xenografts resulted in a 60% increase in uptake in vivo as measured with PET. Conclusions: We have validated the combination of VZV-tk and radiolabeled BCNAs as new PRG/PRP system. Further optimization of the PRPs and the PRG are warranted to increase the signal.

  11. The role of ZAP70 kinase in acute lymphoblastic leukemia infiltration into the central nervous system.

    Science.gov (United States)

    Alsadeq, Ameera; Fedders, Henning; Vokuhl, Christian; Belau, Nele M; Zimmermann, Martin; Wirbelauer, Tim; Spielberg, Steffi; Vossen-Gajcy, Michaela; Cario, Gunnar; Schrappe, Martin; Schewe, Denis M

    2017-02-01

    Central nervous system infiltration and relapse are poorly understood in childhood acute lymphoblastic leukemia. We examined the role of zeta-chain-associated protein kinase 70 in preclinical models of central nervous system leukemia and performed correlative studies in patients. Zeta-chain-associated protein kinase 70 expression in acute lymphoblastic leukemia cells was modulated using short hairpin ribonucleic acid-mediated knockdown or ectopic expression. We show that zeta-chain-associated protein kinase 70 regulates CCR7/CXCR4 via activation of extracellular signal-regulated kinases. High expression of zeta-chain-associated protein kinase 70 in acute lymphoblastic leukemia cells resulted in a higher proportion of central nervous system leukemia in xenografts as compared to zeta-chain-associated protein kinase 70 low expressing counterparts. High zeta-chain-associated protein kinase 70 also enhanced the migration potential towards CCL19/CXCL12 gradients in vitro CCR7 blockade almost abrogated homing of acute lymphoblastic leukemia cells to the central nervous system in xenografts. In 130 B-cell precursor acute lymphoblastic leukemia and 117 T-cell acute lymphoblastic leukemia patients, zeta-chain-associated protein kinase 70 and CCR7/CXCR4 expression levels were significantly correlated. Zeta-chain-associated protein kinase 70 expression correlated with central nervous system disease in B-cell precursor acute lymphoblastic leukemia, and CCR7/CXCR4 correlated with central nervous system involvement in T-cell acute lymphoblastic leukemia patients. In multivariate analysis, zeta-chain-associated protein kinase 70 expression levels in the upper third and fourth quartiles were associated with central nervous system involvement in B-cell precursor acute lymphoblastic leukemia (odds ratio=7.48, 95% confidence interval, 2.06-27.17; odds ratio=6.86, 95% confidence interval, 1.86-25.26, respectively). CCR7 expression in the upper fourth quartile correlated with central

  12. Depotentiation from potentiated synaptic strength in a tristable system of coupled phosphatase and kinase

    Directory of Open Access Journals (Sweden)

    Mengjiao Chen

    2016-10-01

    Full Text Available Long-term potentiation (LTP of synaptic strength is strongly implicated in learning and memory. On the other hand, depotentiation, the reversal of synaptic strength from potentiated LTP state to the pre-LTP level, is required in extinction of the obsolete memory. A generic tristable system, which couples the phosphatase and kinase switches, exclusively explains how moderate and high elevation of intracellular calcium concentration triggers long-term depression (LTD and LTP, respectively. The present study, introducing calcium influx and calcium release from internal store into the tristable system, further show that significant elevation of cytoplasmic calcium concentration switches activation of both kinase and phosphatase to their basal states, thereby depotentiate the synaptic strength. A phase-plane analysis of the combined model was employed to explain the previously reported depotentiation in experiments and predict a threshold-like effect with calcium concentration. The results not only reveal a mechanism of NMDAR- and mGluR-dependent depotentiation, but also predict further experiments about the role of internal calcium store in induction of depotentiation and extinction of established memories.

  13. Clinical review: kinase inhibitors: adverse effects related to the endocrine system.

    Science.gov (United States)

    Lodish, Maya B

    2013-04-01

    The use of kinase inhibitors (KIs) in the treatment of cancer has become increasingly common, and practitioners must be familiar with endocrine-related side effects associated with these agents. This review provides an update to the clinician regarding the management of potential endocrinological effects of KIs. PubMed was employed to identify relevant manuscripts. A review of the literature was conducted, and data were summarized and incorporated. KIs, including small molecule KIs and monoclonal antibodies directed against kinases, have emerged over the past decade as an important class of anticancer agents. KIs specifically interfere with signaling pathways that are dysregulated in certain types of cancers and also target common mechanisms of growth, invasion, metastasis, and angiogenesis. Currently, at least 20 KIs are approved as cancer therapeutics. However, KIs may affect a broad spectrum of targets and may have additional, unidentified mechanisms of action at the cellular level due to overlap between signaling pathways in the tumor cell and endocrine system. Recent reports in the literature have identified side effects associated with KIs, including alterations in thyroid function, bone metabolism, linear growth, gonadal function, fetal development, adrenal function, and glucose metabolism. Clinicians need to monitor the thyroid functions of patients on KIs. In addition, bone density and vitamin D status should be assessed. Special care should be taken to follow linear growth and development in children taking these agents. Clinicians should counsel patients appropriately on the potential adverse effects of KIs on fetal development.

  14. Marketing reporting system

    OpenAIRE

    Hanić Hasan M.

    2004-01-01

    The main components of a developed and good organized marketing information system are: internal reporting system, marketing reporting system, market research system and analytical marketing system. Marketing reporting system provides data and information about changes in business and micro marketing environment. This component of MIS ensures that marketing managers are up-to-date with what is going on, and to be informed about changes in company marketing environment.

  15. Marketing reporting system

    Directory of Open Access Journals (Sweden)

    Hanić Hasan M.

    2004-01-01

    Full Text Available The main components of a developed and good organized marketing information system are: internal reporting system, marketing reporting system, market research system and analytical marketing system. Marketing reporting system provides data and information about changes in business and micro marketing environment. This component of MIS ensures that marketing managers are up-to-date with what is going on, and to be informed about changes in company marketing environment.

  16. National Outbreak Reporting System

    Data.gov (United States)

    U.S. Department of Health & Human Services — The National Outbreak Reporting System (NORS) is a web-based platform designed to support reporting to CDC by local, state, and territorial health departments in the...

  17. Analysis of the complexity of protein kinases within the phloem sieve tube system. Characterization of Cucurbita maxima calmodulin-like domain protein kinase 1.

    Science.gov (United States)

    Yoo, Byung-Chun; Lee, Jung-Youn; Lucas, William J

    2002-05-03

    In angiosperms, functional, mature sieve elements lack nuclei, vacuoles, ribosomes, and most of the endomembrane network. In this study, the complexity, number, and nature of protein kinases within the phloem sap of Cucurbita maxima were investigated to test the hypothesis that the enucleate sieve tube system utilizes a simplified signal transduction network. Supporting evidence was obtained in that only five putative protein kinases (three calcium-independent and two calcium-dependent protein kinases) were detected within the phloem sap extracted from stem tissues. Biochemical methods were used to purify one such calcium-dependent protein kinase. The gene for this C. maxima calmodulin-like domain protein kinase 1 (CmCPK1), was cloned using peptide microsequences. A combination of mass spectrometry, peptide fingerprinting, and amino-terminal sequencing established that, in the phloem sap, CmCPK1 exists as an amino-terminally cleaved protein. A second highly homologous isoform, CmCPK2, was identified, but although transcripts could be detected in the companion cells, peptide fingerprint analysis suggested that CmCPK2 does not enter the phloem sap. Potential substrates for CmCPK1, within the phloem sap, were also detected using an on-membrane phosphorylation assay. Entry of CmCPK1 into sieve elements via plasmodesmata and the potential roles played by these phloem protein kinases are discussed.

  18. Integrated Reporting Information System -

    Data.gov (United States)

    Department of Transportation — The Integrated Reporting Information System (IRIS) is a flexible and scalable web-based system that supports post operational analysis and evaluation of the National...

  19. Creatine kinase in the serum of patients with acute infections of the central nervous system

    DEFF Research Database (Denmark)

    Peterslund, N A; Heinsvig, E M; Christensen, K D

    1985-01-01

    Serum creatine kinase was assessed in 94 consecutive patients without convulsions admitted to hospital due to suspicion of infection of the central nervous system. No reliable discrimination between patients with aseptic and those with bacterial meningitis was obtained. Patients with bacterial...... of bacterial meningitis. The highest serum CK value found in patients with encephalitis was 725 U/l. Reference values for control patients with meningism were 16-269 U/1. In a subset of 9 patients creatine kinase isoenzyme analysis was performed. In all cases only muscle type (MM) isoenzyme was found...

  20. Ketoacidosis With Canagliflozin Prescribed for Phosphoinositide 3-Kinase Inhibitor–Induced Hyperglycemia: A Case Report

    Directory of Open Access Journals (Sweden)

    Christopher Bowman MD

    2017-08-01

    Full Text Available Context . Many phosphoinositide-3-kinase (PI3K inhibitors are under trial for cancer treatment. We present a patient taking taselisib who developed ketoacidosis within 1 week of starting canagliflozin. Case Description . A 69-year-old female patient with no previous history of diabetes mellitus was enrolled in a clinical trial for taselisib therapy in stage IV breast cancer. Hyperglycemia treatment with metformin was insufficient and not tolerated. The addition of canagliflozin daily resulted in ketoacidosis and hospitalization within 1 week. Conclusions . This case report brings together 2 poorly understood and relatively understudied disorders of glucose homeostasis: hyperglycemia due to PI3K inhibition and euglycemic ketoacidosis due to dehydration/SGLT2 inhibition. It demonstrates the complexities of glucose management in the setting of PI3K inhibition. PI3K stimulation (via insulin in this setting is counterintuitive; therefore, non–insulin-mediated therapies (eg, metformin, thiazolidinediones might be favored over insulin-mediated therapies.

  1. [A case of primary central nervous system anaplastic lymphoma kinase positive anaplastic large cell lymphoma manifested as a unilateral pachymeningits].

    Science.gov (United States)

    Fujisawa, Etsuco; Shibayama, Hidehiro; Mitobe, Fumi; Katada, Fumiaki; Sato, Susumu; Fukutake, Toshio

    2017-11-25

    There have been 23 reports of primary central nervous system anaplastic lymphoma kinase (ALK)-positive anaplastic large cell lymphoma in the literature. Here we report the 24th case of a 40-year-old man who presented with occipital headache for one month. His contrast-enhanced brain MRI showed enhancement around the right temporal lobe, which suggested a diagnosis of hypertrophic pachymeningitis. He improved with steroid therapy. After discharge, however, he was readmitted with generalized convulsive seizures. Finally, he was diagnosed as primary central nervous system ALK-positive anaplastic large cell lymphoma by brain biopsy. Primary central nervous system lymphoma invading dura matter can rarely manifests as a unilateral pachymeningitis. Therefore, in case of pachymeningitis, we should pay attention to the possibility of infiltration of lymophoma with meticulous clinical follow-up.

  2. How tyrosine kinase inhibitors impair metabolism and endocrine system function: a systematic updated review.

    Science.gov (United States)

    Breccia, Massimo; Molica, Matteo; Alimena, Giuliana

    2014-12-01

    Tyrosine kinase inhibitors (TKIs) advent has deeply changed the outcome of chronic myeloid leukemia (CML) patients, with improved rates of response and overall survival. However, for this success some patients paid the price of a number of peculiar side effects, the so-called off-target side effects, specific for each one TKI. These effects are due to non-selective inhibition of other tyrosine kinase receptors, such as PDGFR, c-KIT, Src, VEGF. Consequences of this inhibition, some metabolic changes during the treatment with TKIs are reported. Aim of present review is to report metabolic changes and potential mechanisms involved in the pathogenesis related to imatinib, second (nilotinib and dasatinib) and third generation (bosutinib and ponatinib) TKIs. Copyright © 2014 Elsevier Ltd. All rights reserved.

  3. Uranium price reporting systems

    International Nuclear Information System (INIS)

    1987-09-01

    This report describes the systems for uranium price reporting currently available to the uranium industry. The report restricts itself to prices for U 3 O 8 natural uranium concentrates. Most purchases of natural uranium by utilities, and sales by producers, are conducted in this form. The bulk of uranium in electricity generation is enriched before use, and is converted to uranium hexafluoride, UF 6 , prior to enrichment. Some uranium is traded as UF 6 or as enriched uranium, particularly in the 'secondary' market. Prices for UF 6 and enriched uranium are not considered directly in this report. However, where transactions in UF 6 influence the reported price of U 3 O 8 this influence is taken into account. Unless otherwise indicated, the terms uranium and natural uranium used here refer exclusively to U 3 O 8 . (author)

  4. Benzene Monitor System report

    International Nuclear Information System (INIS)

    Livingston, R.R.

    1992-01-01

    Two systems for monitoring benzene in aqueous streams have been designed and assembled by the Savannah River Technology Center, Analytical Development Section (ADS). These systems were used at TNX to support sampling studies of the full-scale open-quotes SRAT/SME/PRclose quotes and to provide real-time measurements of benzene in Precipitate Hydrolysis Aqueous (PHA) simulant. This report describes the two ADS Benzene Monitor System (BMS) configurations, provides data on system operation, and reviews the results of scoping tests conducted at TNX. These scoping tests will allow comparison with other benzene measurement options being considered for use in the Defense Waste Processing Facility (DWPF) laboratory. A report detailing the preferred BMS configuration statistical performance during recent tests has been issued under separate title: Statistical Analyses of the At-line Benzene Monitor Study, SCS-ASG-92-066. The current BMS design, called the At-line Benzene Monitor (ALBM), allows remote measurement of benzene in PHA solutions. The authors have demonstrated the ability to calibrate and operate this system using peanut vials from a standard Hydragard trademark sampler. The equipment and materials used to construct the ALBM are similar to those already used in other applications by the DWPF lab. The precision of this system (±0.5% Relative Standard Deviation (RSD) at 1 sigma) is better than the purge ampersand trap-gas chromatograpy reference method currently in use. Both BMSs provide a direct measurement of the benzene that can be purged from a solution with no sample pretreatment. Each analysis requires about five minutes per sample, and the system operation requires no special skills or training. The analyzer's computer software can be tailored to provide desired outputs. Use of this system produces no waste stream other than the samples themselves (i.e. no organic extractants)

  5. Cyclin-dependent kinase 5, a node protein in diminished tauopathy: a systems biology approach

    Directory of Open Access Journals (Sweden)

    John Fredy Castro-Alvarez

    2014-09-01

    Full Text Available Alzheimer's disease (AD is the most common cause of dementia worldwide. One of the main pathological changes that occurs in AD is the intracellular accumulation of hyperphosphorylated Tau protein in neurons. Cyclin-dependent kinase 5 (CDK5 is one of the major kinases involved in Tau phosphorylation, directly phosphorylating various residues and simultaneously regulating various substrates such as kinases and phosphatases that influence Tau phosphorylation in a synergistic and antagonistic way. It remains unknown how the interaction between CDK5 and its substrates promotes Tau phosphorylation, and systemic approaches are needed that allow an analysis of all the proteins involved. In this review, the role of the CDK5 signaling pathway in Tau hyperphosphorylation is described, an in silico model of the CDK5 signaling pathway is presented. The relationship among these theoretical and computational models shows that the regulation of Tau phosphorylation by PP2A and GSK3β is essential under basal conditions and also describes the leading role of CDK5 under excitotoxic conditions, where silencing of CDK5 can generate changes in these enzymes to reverse a pathological condition that simulates AD.

  6. Bacillus subtilis Two-Component System Sensory Kinase DegS Is Regulated by Serine Phosphorylation in Its Input Domain

    DEFF Research Database (Denmark)

    Jers, Carsten; Kobir, Ahasanul; Søndergaard, Elsebeth Oline

    2011-01-01

    Bacillus subtilis two-component system DegS/U is well known for the complexity of its regulation. The cytosolic sensory kinase DegS does not receive a single predominant input signal like most two-component kinases, instead it integrates a wide array of metabolic inputs that modulate its activity......S phosphorylation can be carried out by at least two B. subtilis Hanks-type kinases in vitro, and this stimulates the phosphate transfer towards DegU. The consequences of this process were studied in vivo, using phosphomimetic (Ser76Asp) and non-phosphorylatable (Ser76Ala) mutants of DegS. In a number...

  7. Sphingosine Kinases and Sphingosine 1-Phosphate Receptors: Signaling and Actions in the Cardiovascular System

    Directory of Open Access Journals (Sweden)

    Alessandro Cannavo

    2017-08-01

    Full Text Available The sphingosine kinases 1 and 2 (SphK1 and 2 catalyze the phosphorylation of the lipid, sphingosine, generating the signal transmitter, sphingosine 1-phosphate (S1P. The activation of such kinases and the subsequent S1P generation and secretion in the blood serum of mammals represent a major checkpoint in many cellular signaling cascades. In fact, activating the SphK/S1P system is critical for cell motility and proliferation, cytoskeletal organization, cell growth, survival, and response to stress. In the cardiovascular system, the physiological effects of S1P intervene through the binding and activation of a family of five highly selective G protein-coupled receptors, called S1PR1-5. Importantly, SphK/S1P signal is present on both vascular and myocardial cells. S1P is a well-recognized survival factor in many tissues. Therefore, it is not surprising that the last two decades have seen a flourishing of interest and investigative efforts directed to obtain additional mechanistic insights into the signaling, as well as the biological activity of this phospholipid, and of its receptors, especially in the cardiovascular system. Here, we will provide an up-to-date account on the structure and function of sphingosine kinases, discussing the generation, release, and function of S1P. Keeping the bull’s eye on the cardiovascular system, we will review the structure and signaling cascades and biological actions emanating from the stimulation of different S1P receptors. We will end this article with a summary of the most recent, experimental and clinical observations targeting S1PRs and SphKs as possible new therapeutic avenues for cardiovascular disorders, such as heart failure.

  8. Erkitinib, a novel EGFR tyrosine kinase inhibitor screened using a ProteoChip system from a phytochemical library

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Eung-Yoon; Choi, Young-Jin [Biochip Research Center, Hoseo University, Asan 336-795 (Korea, Republic of); Innopharmascreen, Inc., Asan 336-795 (Korea, Republic of); Park, Chan-Won [Biochip Research Center, Hoseo University, Asan 336-795 (Korea, Republic of); Dept. of Biological Science, Hoseo University, Asan 336-795 (Korea, Republic of); Kang, In-Cheol, E-mail: ickang@hoseo.edu [Biochip Research Center, Hoseo University, Asan 336-795 (Korea, Republic of); Dept. of Biological Science, Hoseo University, Asan 336-795 (Korea, Republic of); Innopharmascreen, Inc., Asan 336-795 (Korea, Republic of)

    2009-11-20

    Receptor tyrosine kinases (PTKs) play key roles in the pathogenesis of numerous human diseases, including cancer. Therefore PTK inhibitors are currently under intensive investigation as potential drug candidates. Herein, we report on a ProteoChip-based screening of an epidermal growth factor receptor (EGFR) tyrosine kinase (TK) inhibitor, Erkitinibs, from phytochemical libraries. PLC-{gamma}-1 was used as a substrate immobilized on a ProteoChip and incubated with an EGFR kinase to phosphorylate tyrosine residues of the substrate, followed by a fluorescence detection of the substrate recognized by a phospho-specific monoclonal antibody. Erkitinibs inhibited HeLa cell proliferation in a dose-dependent manner. In conclusion, these data suggest that Erkitinibs can be a specific inhibitor of an EGFR kinase and can be further developed as a potent anti-tumor agent.

  9. Erkitinib, a novel EGFR tyrosine kinase inhibitor screened using a ProteoChip system from a phytochemical library

    International Nuclear Information System (INIS)

    Kim, Eung-Yoon; Choi, Young-Jin; Park, Chan-Won; Kang, In-Cheol

    2009-01-01

    Receptor tyrosine kinases (PTKs) play key roles in the pathogenesis of numerous human diseases, including cancer. Therefore PTK inhibitors are currently under intensive investigation as potential drug candidates. Herein, we report on a ProteoChip-based screening of an epidermal growth factor receptor (EGFR) tyrosine kinase (TK) inhibitor, Erkitinibs, from phytochemical libraries. PLC-γ-1 was used as a substrate immobilized on a ProteoChip and incubated with an EGFR kinase to phosphorylate tyrosine residues of the substrate, followed by a fluorescence detection of the substrate recognized by a phospho-specific monoclonal antibody. Erkitinibs inhibited HeLa cell proliferation in a dose-dependent manner. In conclusion, these data suggest that Erkitinibs can be a specific inhibitor of an EGFR kinase and can be further developed as a potent anti-tumor agent.

  10. Integrated system checkout report

    International Nuclear Information System (INIS)

    1991-01-01

    The planning and preparation phase of the Integrated Systems Checkout Program (ISCP) was conducted from October 1989 to July 1991. A copy of the ISCP, DOE-WIPP 90--002, is included in this report as an appendix. The final phase of the Checkout was conducted from July 10, 1991, to July 23, 1991. This phase exercised all the procedures and equipment required to receive, emplace, and retrieve contact handled transuranium (CH TRU) waste filled dry bins. In addition, abnormal events were introduced to simulate various equipment failures, loose surface radioactive contamination events, and personnel injury. This report provides a detailed summary of each days activities during this period. Qualification of personnel to safely conduct the tasks identified in the procedures and the abnormal events were verified by observers familiar with the Bin-Scale CH TRU Waste Test requirements. These observers were members of the staffs of Westinghouse WID Engineering, QA, Training, Health Physics, Safety, and SNL. Observers representing a number of DOE departments, the state of new Mexico, and the Defense Nuclear Facilities Safety Board observed those Checkout activities conducted during the period from July 17, 1991, to July 23, 1991. Observer comments described in this report are those obtained from the staff member observers. 1 figs., 1 tab

  11. Roles of the creatine kinase system and myoglobin in maintaining energetic state in the working heart

    Directory of Open Access Journals (Sweden)

    Beard Daniel A

    2009-02-01

    Full Text Available Abstract Background The heart is capable of maintaining contractile function despite a transient decrease in blood flow and increase in cardiac ATP demand during systole. This study analyzes a previously developed model of cardiac energetics and oxygen transport to understand the roles of the creatine kinase system and myoglobin in maintaining the ATP hydrolysis potential during beat-to-beat transient changes in blood flow and ATP hydrolysis rate. Results The theoretical investigation demonstrates that elimination of myoglobin only slightly increases the predicted range of oscillation of cardiac oxygenation level during beat-to-beat transients in blood flow and ATP utilization. In silico elimination of myoglobin has almost no impact on the cytoplasmic ATP hydrolysis potential (ΔGATPase. In contrast, disabling the creatine kinase system results in considerable oscillations of cytoplasmic ADP and ATP levels and seriously deteriorates the stability of ΔGATPase in the beating heart. Conclusion The CK system stabilizes ΔGATPase by both buffering ATP and ADP concentrations and enhancing the feedback signal of inorganic phosphate in regulating mitochondrial oxidative phosphorylation.

  12. Exceptionally High Creatine Kinase (CK) Levels in Multicausal and Complicated Rhabdomyolysis: A Case Report.

    Science.gov (United States)

    Luckoor, Pavan; Salehi, Mashal; Kunadu, Afua

    2017-07-04

    BACKGROUND Rhabdomyolysis is a syndrome caused by muscle breakdown. It can be caused by traumatic as well as non-traumatic factors such as drugs, toxins, and infections. Although it has been initially associated with only traumatic causes, non-traumatic causes now appear to be at least 5 times more frequent. In rhabdomyolysis, the CK levels can range anywhere from 10 000 to 200 000 or even higher. The higher the CK levels, the greater will be the renal damage and associated complications. We present the case of a patient with exceptionally massive rhabdomyolysis with unusually high CK levels (nearly 1 million) caused by combined etiologic factors and complicated with acute renal failure. CASE REPORT A 36-year-old African American male patient with no significant past medical history and a social history of cocaine and alcohol abuse presented with diarrhea and generalized weakness of 2 days' duration. He was found to be febrile, tachycardic, tachypneic, and hypoxic. The patient was subsequently intubated and admitted to the medical ICU. Laboratory work-up showed acute renal failure with deranged liver functions test results, and elevated creatine kinase of 701,400 U/L. CK levels were subsequently too high for the lab to quantify. Urine legionella testing was positive for L. pneumophilia serogroup 1 antigen and urine toxicology was positive for cocaine. The patient had a protracted course in the ICU. He was initially started on CVVH, and later received intermittent hemodialysis for about 1 month. CONCLUSIONS In the presence of multiple etiologic factors, rhabdomyolysis can be massive with resultant significant morbidity. Clinicians should have a high index of suspicion for rhabdomyolysis in the presence of multiple factors, as early recognition of this diseases is very important in the prevention and active management of life-threatening conditions.

  13. Fluorescent Reporters and Biosensors for Probing the Dynamic Behavior of Protein Kinases

    Directory of Open Access Journals (Sweden)

    Juan A. González-Vera

    2015-11-01

    Full Text Available Probing the dynamic activities of protein kinases in real-time in living cells constitutes a major challenge that requires specific and sensitive tools tailored to meet the particular demands associated with cellular imaging. The development of genetically-encoded and synthetic fluorescent biosensors has provided means of monitoring protein kinase activities in a non-invasive fashion in their native cellular environment with high spatial and temporal resolution. Here, we review existing technologies to probe different dynamic features of protein kinases and discuss limitations where new developments are required to implement more performant tools, in particular with respect to infrared and near-infrared fluorescent probes and strategies which enable improved signal-to-noise ratio and controlled activation of probes.

  14. Systems biology analysis of mitogen activated protein kinase inhibitor resistance in malignant melanoma.

    Science.gov (United States)

    Zecena, Helma; Tveit, Daniel; Wang, Zi; Farhat, Ahmed; Panchal, Parvita; Liu, Jing; Singh, Simar J; Sanghera, Amandeep; Bainiwal, Ajay; Teo, Shuan Y; Meyskens, Frank L; Liu-Smith, Feng; Filipp, Fabian V

    2018-04-04

    Kinase inhibition in the mitogen activated protein kinase (MAPK) pathway is a standard therapy for cancer patients with activating BRAF mutations. However, the anti-tumorigenic effect and clinical benefit are only transient, and tumors are prone to treatment resistance and relapse. To elucidate mechanistic insights into drug resistance, we have established an in vitro cellular model of MAPK inhibitor resistance in malignant melanoma. The cellular model evolved in response to clinical dosage of the BRAF inhibitor, vemurafenib, PLX4032. We conducted transcriptomic expression profiling using RNA-Seq and RT-qPCR arrays. Pathways of melanogenesis, MAPK signaling, cell cycle, and metabolism were significantly enriched among the set of differentially expressed genes of vemurafenib-resistant cells vs control. The underlying mechanism of treatment resistance and pathway rewiring was uncovered to be based on non-genomic adaptation and validated in two distinct melanoma models, SK-MEL-28 and A375. Both cell lines have activating BRAF mutations and display metastatic potential. Downregulation of dual specific phosphatases, tumor suppressors, and negative MAPK regulators reengages mitogenic signaling. Upregulation of growth factors, cytokines, and cognate receptors triggers signaling pathways circumventing BRAF blockage. Further, changes in amino acid and one-carbon metabolism support cellular proliferation despite MAPK inhibitor treatment. In addition, treatment-resistant cells upregulate pigmentation and melanogenesis, pathways which partially overlap with MAPK signaling. Upstream regulator analysis discovered significant perturbation in oncogenic forkhead box and hypoxia inducible factor family transcription factors. The established cellular models offer mechanistic insight into cellular changes and therapeutic targets under inhibitor resistance in malignant melanoma. At a systems biology level, the MAPK pathway undergoes major rewiring while acquiring inhibitor resistance

  15. Fuels Reporting System Data

    Data.gov (United States)

    U.S. Environmental Protection Agency — This asset includes compliance data (registrations and reports), including reports related to reformulated gasoline and conventional gasoline (anti-dumping),...

  16. Physician Quality Reporting System

    Data.gov (United States)

    U.S. Department of Health & Human Services — PQRS is a reporting program that uses a combination of incentive payments and negative payment adjustments to promote reporting of quality information by eligible...

  17. The aviation safety reporting system

    Science.gov (United States)

    Reynard, W. D.

    1984-01-01

    The aviation safety reporting system, an accident reporting system, is presented. The system identifies deficiencies and discrepancies and the data it provides are used for long term identification of problems. Data for planning and policy making are provided. The system offers training in safety education to pilots. Data and information are drawn from the available data bases.

  18. Cancer metabolism meets systems biology: Pyruvate kinase isoform PKM2 is a metabolic master regulator

    OpenAIRE

    Fabian V Filipp

    2013-01-01

    Pyruvate kinase activity is controlled by a tightly woven regulatory network. The oncofetal isoform of pyruvate kinase (PKM2) is a master regulator of cancer metabolism. PKM2 engages in parallel, feed-forward, positive and negative feedback control contributing to cancer progression. Besides its metabolic role, non-metabolic functions of PKM2 as protein kinase and transcriptional coactivator for c-MYC and hypoxia-inducible factor 1-alpha are essential for epidermal growth factor receptor acti...

  19. AMP-Activated Protein Kinase: An Ubiquitous Signaling Pathway With Key Roles in the Cardiovascular System.

    Science.gov (United States)

    Salt, Ian P; Hardie, D Grahame

    2017-05-26

    The AMP-activated protein kinase (AMPK) is a key regulator of cellular and whole-body energy homeostasis, which acts to restore energy homoeostasis whenever cellular energy charge is depleted. Over the last 2 decades, it has become apparent that AMPK regulates several other cellular functions and has specific roles in cardiovascular tissues, acting to regulate cardiac metabolism and contractile function, as well as promoting anticontractile, anti-inflammatory, and antiatherogenic actions in blood vessels. In this review, we discuss the role of AMPK in the cardiovascular system, including the molecular basis of mutations in AMPK that alter cardiac physiology and the proposed mechanisms by which AMPK regulates vascular function under physiological and pathophysiological conditions. © 2017 American Heart Association, Inc.

  20. Revised licensee event report system

    International Nuclear Information System (INIS)

    Mays, G.T.; Poore, W.P.

    1985-01-01

    Licensee Event Reports (LERs) provide the basis for evaluating and assessing operating experience information from nuclear power plants. The reporting requirements for submitting LERs to the Nuclear Regulatory Commission have been revised. Effective Jan. 1, 1984, all events were to be submitted in accordance with 10 CFR 50.73 of the Code of Federal Regulations. Report NUREG-1022, Licensee Event Report System-Description of System and Guidelines for Reporting, describes the guidelines on reportability of events. This article summarizes the reporting requirements as presented in NUREG-1022, high-lights differences in data reported between the revised and previous LER systems, and presents results from a preliminary assessment of LERs submitted under the revised LER reporting system

  1. Protein Kinase Mitogen-activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) Promotes Obesity-induced Hyperinsulinemia.

    Science.gov (United States)

    Roth Flach, Rachel J; Danai, Laura V; DiStefano, Marina T; Kelly, Mark; Menendez, Lorena Garcia; Jurczyk, Agata; Sharma, Rohit B; Jung, Dae Young; Kim, Jong Hun; Kim, Jason K; Bortell, Rita; Alonso, Laura C; Czech, Michael P

    2016-07-29

    Previous studies revealed a paradox whereby mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4) acted as a negative regulator of insulin sensitivity in chronically obese mice, yet systemic deletion of Map4k4 did not improve glucose tolerance. Here, we report markedly reduced glucose-responsive plasma insulin and C-peptide levels in whole body Map4k4-depleted mice (M4K4 iKO) as well as an impaired first phase of insulin secretion from islets derived from M4K4 iKO mice ex vivo After long-term high fat diet (HFD), M4K4 iKO mice pancreata also displayed reduced β cell mass, fewer proliferating β cells and reduced islet-specific gene mRNA expression compared with controls, although insulin content was normal. Interestingly, the reduced plasma insulin in M4K4 iKO mice exposed to chronic (16 weeks) HFD was not observed in response to acute HFD challenge or short term treatment with the insulin receptor antagonist S961. Furthermore, the improved insulin sensitivity in obese M4K4 iKO mice was abrogated by high exogenous insulin over the course of a euglycemic clamp study, indicating that hypoinsulinemia promotes insulin sensitivity in chronically obese M4K4 iKO mice. These results demonstrate that protein kinase Map4k4 drives obesity-induced hyperinsulinemia and insulin resistance in part by promoting insulin secretion from β cells in mice. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

  2. A Calcium-Dependent Protein Kinase Is Systemically Induced upon Wounding in Tomato Plants1

    Science.gov (United States)

    Chico, José Manuel; Raíces, Marcela; Téllez-Iñón, María Teresa; Ulloa, Rita María

    2002-01-01

    A full-length cDNA clone (LeCDPK1) from tomato (Lycopersicon esculentum) encoding a calcium-dependent protein kinase (CDPK) was isolated by screening a cDNA library from tomato cell cultures exposed to Cladosporium fulvum elicitor preparations. The predicted amino acid sequence of the cDNA reveals a high degree of similarity with other members of the CDPK family. LeCDPK1 has a putative N-terminal myristoylation sequence and presents a possible palmitoylation site. The in vitro translated protein conserves the biochemical properties of a member of the CDPK family. In addition, CDPK activity was detected in soluble and particulate extracts of tomato leaves. Basal levels of LeCDPK1 mRNA were detected by northern-blot analysis in roots, stems, leaves, and flowers of tomato plants. The expression of LeCDPK1 was rapidly and transiently enhanced in detached tomato leaves treated with pathogen elicitors and H2O2. Moreover, when tomato greenhouse plants were subjected to mechanical wounding, a transient increase of LeCDPK1 steady-state mRNA levels was detected locally at the site of the injury and systemically in distant non-wounded leaves. The increase observed in LeCDPK1 mRNA upon wounding correlates with an increase in the amount and in the activity of a soluble CDPK detected in extracts of tomato leaves, suggesting that this kinase is part of physiological plant defense mechanisms against biotic or abiotic attacks. PMID:11788771

  3. Congestion Management System Process Report

    Science.gov (United States)

    1996-03-01

    In January 1995, the Indianapolis Metropolitan Planning Organization with the help of an interagency Study Review Committee began the process of developing a Congestion Management System (CMS) Plan resulting in this report. This report documents the ...

  4. Contractor Performance Assessment Reporting System

    Data.gov (United States)

    US Agency for International Development — CPARS is a web-based system used to input data on contractor performance. Reports from the system are used as an aid in awarding contracts to contractors that...

  5. Roles of A-Kinase Anchoring Proteins and Phosphodiesterases in the Cardiovascular System

    Science.gov (United States)

    Ercu, Maria; Klussmann, Enno

    2018-01-01

    A-kinase anchoring proteins (AKAPs) and cyclic nucleotide phosphodiesterases (PDEs) are essential enzymes in the cyclic adenosine 3′-5′ monophosphate (cAMP) signaling cascade. They establish local cAMP pools by controlling the intensity, duration and compartmentalization of cyclic nucleotide-dependent signaling. Various members of the AKAP and PDE families are expressed in the cardiovascular system and direct important processes maintaining homeostatic functioning of the heart and vasculature, e.g., the endothelial barrier function and excitation-contraction coupling. Dysregulation of AKAP and PDE function is associated with pathophysiological conditions in the cardiovascular system including heart failure, hypertension and atherosclerosis. A number of diseases, including autosomal dominant hypertension with brachydactyly (HTNB) and type I long-QT syndrome (LQT1), result from mutations in genes encoding for distinct members of the two classes of enzymes. This review provides an overview over the AKAPs and PDEs relevant for cAMP compartmentalization in the heart and vasculature and discusses their pathophysiological role as well as highlights the potential benefits of targeting these proteins and their protein-protein interactions for the treatment of cardiovascular diseases. PMID:29461511

  6. Water Fluoridation Reporting System (Public Water Systems)

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Water Fluoridation Reporting System (WFRS) has been developed to provide tools to assist states in managing fluoridation programs. WFRS is designed to track all...

  7. Conditional deletion of ERK5 MAP kinase in the nervous system impairs pheromone information processing and pheromone-evoked behaviors.

    Directory of Open Access Journals (Sweden)

    Junhui Zou

    Full Text Available ERK5 MAP kinase is highly expressed in the developing nervous system but absent in most regions of the adult brain. It has been implicated in regulating the development of the main olfactory bulb and in odor discrimination. However, whether it plays an essential role in pheromone-based behavior has not been established. Here we report that conditional deletion of the Mapk7 gene which encodes ERK5 in mice in neural stem cells impairs several pheromone-mediated behaviors including aggression and mating in male mice. These deficits were not caused by a reduction in the level of testosterone, by physical immobility, by heightened fear or anxiety, or by depression. Using mouse urine as a natural pheromone-containing solution, we provide evidence that the behavior impairment was associated with defects in the detection of closely related pheromones as well as with changes in their innate preference for pheromones related to sexual and reproductive activities. We conclude that expression of ERK5 during development is critical for pheromone response and associated animal behavior in adult mice.

  8. Association between raf kinase inhibitor protein loss and prognosis in cancers of the digestive system: a meta-analysis.

    Science.gov (United States)

    Yu, Min; Wang, Qian; Ding, Jiang-Wu; Yang, Zhen; Xie, Chuan; Lu, Nong-Hua

    2014-01-01

    Loss of Raf kinase inhibitor protein (RKIP) may contribute to metastasis in a variety of human cancers. Many studies have evaluated whether loss of RKIP expression is a prognostic factor for survival in cancers of the digestive system, however, its predictive value remains controversial. Thus, we performed a meta-analysis to obtain a more comprehensive estimate of the prognostic value of RKIP expression in digestive system cancers. Studies were identified by searching multiple electronic databases through December 12, 2013, and by reviewing reference lists of obtained articles. Studies reported hazard ratios (HRs) with 95% confidence intervals (CIs) for the association between RKIP and overall survival (OS) and disease-free survival (DFS) in cancers of the digestive system were eligible, including esophageal cancer, gastric cancer, colorectal cancer and pancreatic cancer. Nineteen studies involving approximately 3700 participants were included in the final analysis. The pooled results suggested that loss of RKIP expression was associated with unfavorable OS (HR 0.55, 95% CI 0.46-0.65) and DFS (HR 0.46, 95% CI 0.30-0.62) among patients with digestive system cancers, whereas the difference was not statistically significant in pancreatic cancer specifically (OS, HR 0.76; 95% CI 0.51-1.01; DFS, HR 0.71; 95% CI 0.28-1.13). Loss of RKIP expression might be an independent indicator of poor prognosis in patients with digestive tract cancers, which includes esophageal cancer, gastric cancer and colorectal cancer. More studies are needed to further clarify the prognostic value of RKIP in pancreatic cancer. Future studies, preferably large prospective studies utilizing formal marker assessment processes, are needed to establish the prognostic value of RKIP before these results can be clinically applied.

  9. Adverse Event Reporting System (AERS)

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Adverse Event Reporting System (AERS) is a computerized information database designed to support the FDA's post-marketing safety surveillance program for all...

  10. Vessel Electronic Reporting System (VERS)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The VERS system is composed of a database and other related applications which facilitate the reporting of electronically collected research data via Fisheries...

  11. SPECTR System Operational Test Report

    International Nuclear Information System (INIS)

    Landman, W.H. Jr.

    2011-01-01

    This report overviews installation of the Small Pressure Cycling Test Rig (SPECTR) and documents the system operational testing performed to demonstrate that it meets the requirements for operations. The system operational testing involved operation of the furnace system to the design conditions and demonstration of the test article gas supply system using a simulated test article. The furnace and test article systems were demonstrated to meet the design requirements for the Next Generation Nuclear Plant. Therefore, the system is deemed acceptable and is ready for actual test article testing.

  12. 2014 Runtime Systems Summit. Runtime Systems Report

    Energy Technology Data Exchange (ETDEWEB)

    Sarkar, Vivek [US Dept. of Energy, Washington, DC (United States); Budimlic, Zoran [US Dept. of Energy, Washington, DC (United States); Kulkani, Milind [US Dept. of Energy, Washington, DC (United States)

    2016-09-19

    This report summarizes runtime system challenges for exascale computing, that follow from the fundamental challenges for exascale systems that have been well studied in past reports, e.g., [6, 33, 34, 32, 24]. Some of the key exascale challenges that pertain to runtime systems include parallelism, energy efficiency, memory hierarchies, data movement, heterogeneous processors and memories, resilience, performance variability, dynamic resource allocation, performance portability, and interoperability with legacy code. In addition to summarizing these challenges, the report also outlines different approaches to addressing these significant challenges that have been pursued by research projects in the DOE-sponsored X-Stack and OS/R programs. Since there is often confusion as to what exactly the term “runtime system” refers to in the software stack, we include a section on taxonomy to clarify the terminology used by participants in these research projects. In addition, we include a section on deployment opportunities for vendors and government labs to build on the research results from these projects. Finally, this report is also intended to provide a framework for discussing future research and development investments for exascale runtime systems, and for clarifying the role of runtime systems in exascale software.

  13. STATE OF JNK AND P38 MAP-KINASE SYSTEM IN BLOOD monon uclea r le ucocytes DUR ING INFLAMMATION

    Directory of Open Access Journals (Sweden)

    N. Y. Chasovskih

    2009-01-01

    Full Text Available Abstract. Pogrammed cell death of peripheral blood mononuclear leucocytes from patients with acute inflammatory diseases (non-nosocomial pneumonia, acute appendicitis was investigated under ex vivo conditions, upon cultivation of the cells with selective inhibitors of JNK (SP600125 and р38 МАРК (ML3403. In vitro addition of SP600125 and ML3403 under oxidative stress conditions prevents increase of annexinpositive mononuclear cells numbers, thus suggesting JNK and р38 МАР-kinases to be involved into oxidative mechanisms of apoptosis deregulation. A role of JNK in IL-8 production by mononuclear leucocytes was revealed in cases of acute inflammation. Regulatory effect of JNK and p38 MAP-kinases can be mediated through activation of redox-sensitive apoptogenic signal transduction systems, as well as due to changes in cellular cytokine-producing function.

  14. Serine/threonine kinase 15 gene polymorphism and risk of digestive system cancers: A meta-analysis.

    Science.gov (United States)

    Luo, Jianfei; Yan, Ruicheng; Zou, Li

    2015-01-01

    Previous studies have reported an association between the two coding polymorphisms (91T>A and 169G>A) of the serine/threonine kinase 15 (STK15) gene and the risk of digestive system cancers; however, the results are inconsistent. In the present study, a meta-analysis was carried out to assess the association between the two STK15 polymorphisms and the risk of digestive system cancers. Relevant studies were identified using PubMed, Web of Science, China National Knowledge Infrastructure, WanFang and VIP databases up to February 18, 2014. The pooled odds ratio (OR) with a 95% confidence interval (CI) was calculated using the fixed or random effects model. A total of 15 case-control studies from 14 publications were included. Of these, 15 studies concerned the 91T>A polymorphism and included 7,619 cases and 7,196 controls and four studies concerned the 161G>A polymorphism and included 826 cases and 713 controls. A significantly increased risk of digestive system cancers was observed for the 91T>A polymorphism (recessive model: OR, 1.19; 95% CI, 1.07-1.31). In subgroup analysis by ethnicity, a significant association was detected in Asian populations (recessive model: OR, 1.21; 95% CI, 1.08-1.36) but not in Caucasian and mixed populations. Stratification by tumor type indicated that the 91T>A polymorphism was associated with an increased risk of esophageal and colorectal cancers under the recessive model (OR, 1.19; 95% CI, 1.03-1.38; and OR, 1.24; 95% CI, 1.04-1.46; respectively); however, no significant association was observed between the 169G>A polymorphism and the risk of digestive system cancers in any of the genetic models. Furthermore, in subgroup analysis by ethnicity, similar results were observed in the Asian and Caucasian populations. The present meta-analysis demonstrated that the STK15 gene 91T>A polymorphism, but not the 169G>A polymorphism, may be a risk factor for digestive system cancers, particularly for esophageal and colorectal cancers.

  15. Experimental lithium system. Final report

    International Nuclear Information System (INIS)

    Kolowith, R.; Berg, J.D.; Miller, W.C.

    1985-04-01

    A full-scale mockup of the Fusion Materials Irradiation Test (FMIT) Facility lithium system was built at the Hanford Engineering Development Laboratory (HEDL). This isothermal mockup, called the Experimental Lithium System (ELS), was prototypic of FMIT, excluding the accelerator and dump heat exchanger. This 3.8 m 3 lithium test loop achieved over 16,000 hours of safe and reliable operation. An extensive test program demonstrated satisfactory performance of the system components, including the HEDL-supplied electromagnetic lithium pump, the lithium jet target, the purification and characterization hardware, as well as the auxiliary argon and vacuum systems. Experience with the test loop provided important information on system operation, performance, and reliability. This report presents a complete overview of the entire Experimental Lithium System test program and also includes a summary of such areas as instrumentation, coolant chemistry, vapor/aerosol transport, and corrosion

  16. Subscriber Response System. Progress Report.

    Science.gov (United States)

    Callais, Richard T.

    Results of preliminary tests made prior and subsequent to the installation of a two-way interactive communication system which involves a computer complex termed the Local Processing Center and subscriber terminals located in the home or business location are reported. This first phase of the overall test plan includes tests made at Theta-Com…

  17. NASA aviation safety reporting system

    Science.gov (United States)

    1981-01-01

    Aviation safety reports that relate to loss of control in flight, problems that occur as a result of similar sounding alphanumerics, and pilot incapacitation are presented. Problems related to the go around maneuver in air carrier operations, and bulletins (and FAA responses to them) that pertain to air traffic control systems and procedures are included.

  18. The NSTX Trouble Reporting System

    International Nuclear Information System (INIS)

    Sengupta, S.; Oliaro, G.

    2002-01-01

    An online Trouble Reporting System (TRS) has been introduced at the National Spherical Torus Experiment (NSTX). The TRS is used by NSTX operators to report problems that affect NSTX operations. The purpose of the TRS is to enhance NSTX reliability and maintainability by identifying components, occurrences, and trends that contribute to machine downtime. All NSTX personnel have access to the TRS. The user interface is via a web browser, such as Netscape or Internet Explorer. This web-based feature permits any X-terminal, PC, or MAC access to the TRS. The TRS is based upon a trouble reporting system developed at the DIII-D Tokamak, at General Atomics Technologies. This paper will provide a detailed description of the TRS software architecture, user interface, MS SQL server interface and operational experiences. In addition, sample data from the TRS database will be summarized and presented

  19. Registered report: Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors2

    OpenAIRE

    sprotocols

    2015-01-01

    Authors: Edward Greenfield, Erin Griner, The Reproducibility Project: Cancer Biology† ### Abstract The [Reproducibility Project: Cancer Biology](https://osf.io/e81xl/wiki/home/) seeks to address growing concerns about reproducibility in scientific research by conducting replications of 50 papers in the field of cancer biology published between 2010 and 2012. This Registered Report describes the proposed replication plan of key experiments from “Widespread potential for growth-factor-d...

  20. A screen for kinase inhibitors identifies antimicrobial imidazopyridine aminofurazans as specific inhibitors of the Listeria monocytogenes PASTA kinase PrkA.

    Science.gov (United States)

    Schaenzer, Adam J; Wlodarchak, Nathan; Drewry, David H; Zuercher, William J; Rose, Warren E; Striker, Rob; Sauer, John-Demian

    2017-10-13

    Bacterial signaling systems such as protein kinases and quorum sensing have become increasingly attractive targets for the development of novel antimicrobial agents in a time of rising antibiotic resistance. The family of bacterial P enicillin-binding-protein A nd S erine/ T hreonine kinase- A ssociated (PASTA) kinases is of particular interest due to the role of these kinases in regulating resistance to β-lactam antibiotics. As such, small-molecule kinase inhibitors that target PASTA kinases may prove beneficial as treatments adjunctive to β-lactam therapy. Despite this interest, only limited progress has been made in identifying functional inhibitors of the PASTA kinases that have both activity against the intact microbe and high kinase specificity. Here, we report the results of a small-molecule screen that identified GSK690693, an imidazopyridine aminofurazan-type kinase inhibitor that increases the sensitivity of the intracellular pathogen Listeria monocytogenes to various β-lactams by inhibiting the PASTA kinase PrkA. GSK690693 potently inhibited PrkA kinase activity biochemically and exhibited significant selectivity for PrkA relative to the Staphylococcus aureus PASTA kinase Stk1. Furthermore, other imidazopyridine aminofurazans could effectively inhibit PrkA and potentiate β-lactam antibiotic activity to varying degrees. The presence of the 2-methyl-3-butyn-2-ol (alkynol) moiety was important for both biochemical and antimicrobial activity. Finally, mutagenesis studies demonstrated residues in the back pocket of the active site are important for GSK690693 selectivity. These data suggest that targeted screens can successfully identify PASTA kinase inhibitors with both biochemical and antimicrobial specificity. Moreover, the imidazopyridine aminofurazans represent a family of PASTA kinase inhibitors that have the potential to be optimized for selective PASTA kinase inhibition.

  1. Comparative analysis of LytS/LytTR-type histidine kinase/response regulator systems in γ-proteobacteria.

    Directory of Open Access Journals (Sweden)

    Stefan Behr

    Full Text Available Bacterial histidine kinase/response regulator systems operate at the interface between environmental cues and physiological states. Escherichia coli contains two LytS/LytTR-type histidine kinase/response regulator systems, BtsS/BtsR (formerly YehU/YehT and YpdA/YpdB, which have been identified as pyruvate-responsive two-component systems. Since they exhibit remarkable similarity, we analyzed their phylogenetic distribution within the γ-proteobacteria, and experimentally characterized them in a set of representative species. We found that BtsS/BtsR is the predominant LytS/LytTR-type two-component system among γ-proteobacteria, whereas YpdA/YpdB primarily appears in a supplementary role. Based on our observations in E. coli, we used the highly conserved DNA-binding motifs to test the in vivo functionality of both systems in various genera, including Salmonella, Enterobacter, Citrobacter, Xenorhabdus, Yersinia, Aeromonas and Vibrio. The results suggest that, in all cases tested, BtsS/BtsR and YpdA/YpdB respond to different levels of pyruvate in the environment.

  2. Interaction of renin-angiotensin system and adenosine monophosphate-activated protein kinase signaling pathway in renal carcinogenesis of uninephrectomized rats.

    Science.gov (United States)

    Yang, Ke-Ke; Sui, Yi; Zhou, Hui-Rong; Zhao, Hai-Lu

    2017-05-01

    Renin-angiotensin system and adenosine monophosphate-activated protein kinase signaling pathway both play important roles in carcinogenesis, but the interplay of renin-angiotensin system and adenosine monophosphate-activated protein kinase in carcinogenesis is not clear. In this study, we researched the interaction of renin-angiotensin system and adenosine monophosphate-activated protein kinase in renal carcinogenesis of uninephrectomized rats. A total of 96 rats were stratified into four groups: sham, uninephrectomized, and uninephrectomized treated with angiotensin-converting enzyme inhibitor or angiotensin receptor blocker. Renal adenosine monophosphate-activated protein kinase and its downstream molecule acetyl coenzyme A carboxylase were detected by immunohistochemistry and western blot at 10 months after uninephrectomy. Meanwhile, we examined renal carcinogenesis by histological transformation and expressions of Ki67 and mutant p53. During the study, fasting lipid profiles were detected dynamically at 3, 6, 8, and 10 months. The results indicated that adenosine monophosphate-activated protein kinase expression in uninephrectomized rats showed 36.8% reduction by immunohistochemistry and 89.73% reduction by western blot. Inversely, acetyl coenzyme A carboxylase expression increased 83.3% and 19.07% in parallel to hyperlipidemia at 6, 8, and 10 months. The histopathology of carcinogenesis in remnant kidneys was manifested by atypical proliferation and carcinoma in situ, as well as increased expressions of Ki67 and mutant p53. Intervention with angiotensin-converting enzyme inhibitor or angiotensin receptor blocker significantly prevented the inhibition of adenosine monophosphate-activated protein kinase signaling pathway and renal carcinogenesis in uninephrectomized rats. In conclusion, the novel findings suggest that uninephrectomy-induced disturbance in adenosine monophosphate-activated protein kinase signaling pathway resulted in hyperlipidemia and

  3. Systems Analysis of Adaptive Responses to MAP Kinase Pathway Blockade in BRAF Mutant Melanoma.

    Directory of Open Access Journals (Sweden)

    Brian J Capaldo

    Full Text Available Fifty percent of cutaneous melanomas are driven by activated BRAFV600E, but tumors treated with RAF inhibitors, even when they respond dramatically, rapidly adapt and develop resistance. Thus, there is a pressing need to identify the major mechanisms of intrinsic and adaptive resistance and develop drug combinations that target these resistance mechanisms. In a combinatorial drug screen on a panel of 12 treatment-naïve BRAFV600E mutant melanoma cell lines of varying levels of resistance to mitogen-activated protein kinase (MAPK pathway inhibition, we identified the combination of PLX4720, a targeted inhibitor of mutated BRaf, and lapatinib, an inhibitor of the ErbB family of receptor tyrosine kinases, as synergistically cytotoxic in the subset of cell lines that displayed the most resistance to PLX4720. To identify potential mechanisms of resistance to PLX4720 treatment and synergy with lapatinib treatment, we performed a multi-platform functional genomics analysis to profile the genome as well as the transcriptional and proteomic responses of these cell lines to treatment with PLX4720. We found modest levels of resistance correlated with the zygosity of the BRAF V600E allele and receptor tyrosine kinase (RTK mutational status. Layered over base-line resistance was substantial upregulation of many ErbB pathway genes in response to BRaf inhibition, thus generating the vulnerability to combination with lapatinib. The transcriptional responses of ErbB pathway genes are associated with a number of transcription factors, including ETS2 and its associated cofactors that represent a convergent regulatory mechanism conferring synergistic drug susceptibility in the context of diverse mutational landscapes.

  4. A Noninvasive In Vitro Monitoring System Reporting Skeletal Muscle Differentiation.

    Science.gov (United States)

    Öztürk-Kaloglu, Deniz; Hercher, David; Heher, Philipp; Posa-Markaryan, Katja; Sperger, Simon; Zimmermann, Alice; Wolbank, Susanne; Redl, Heinz; Hacobian, Ara

    2017-01-01

    Monitoring of cell differentiation is a crucial aspect of cell-based therapeutic strategies depending on tissue maturation. In this study, we have developed a noninvasive reporter system to trace murine skeletal muscle differentiation. Either a secreted bioluminescent reporter (Metridia luciferase) or a fluorescent reporter (green fluorescent protein [GFP]) was placed under the control of the truncated muscle creatine kinase (MCK) basal promoter enhanced by variable numbers of upstream MCK E-boxes. The engineered pE3MCK vector, coding a triple tandem of E-Boxes and the truncated MCK promoter, showed twentyfold higher levels of luciferase activation compared with a Cytomegalovirus (CMV) promoter. This newly developed reporter system allowed noninvasive monitoring of myogenic differentiation in a straining bioreactor. Additionally, binding sequences of endogenous microRNAs (miRNAs; seed sequences) that are known to be downregulated in myogenesis were ligated as complementary seed sequences into the reporter vector to reduce nonspecific signal background. The insertion of seed sequences improved the signal-to-noise ratio up to 25% compared with pE3MCK. Due to the highly specific, fast, and convenient expression analysis for cells undergoing myogenic differentiation, this reporter system provides a powerful tool for application in skeletal muscle tissue engineering.

  5. Synthesis report: System studies Bioenergy

    International Nuclear Information System (INIS)

    Berntsson, Thore

    2003-01-01

    The present report marks the end of the research program 'System studies Bioenergy' (1998-2002). The program comprised 17 projects performed at 9 universities or research institutes. All project results were studied in order to identify: contributions to our present knowledge; possible gaps of knowledge, methodology or systems perspective that still exist; and the needs for further research. The projects can be classified into the following groups: Resource potential of forest fuels; Industrial use of biofuels; Potential for synthetic fuels (pellets, bio-oils and transportation fuels); System analysis of efficient use of biofuels; and Socio-economic analyses. The total potential for available biofuel has been estimated to be 125-175 TWh/year (excl. black liquors of paper industry). The potential demand is estimated to about 123 TWh/year, or distributed into the different sectors: Industry: 26 TWh/year, Buildings and services: 35 TWh/year, District heating: 31 TWh/year, and electric power generation (incl. cogeneration in district heating): 31 TWh/year. Further research is needed in the following areas: Systems and methodology of more generic character on optimization of production, refining and use of biofuels in order to substitute fossil fuels directly or indirectly; Heat sinks/district heating in combination with cogeneration vs. other power production in a long term perspective (> 10 years), in the light of new technologies, open markets, economic and political incentives; Energy efficiency in industry, esp. paper and pulp with its unique possibility for process integration, biofuel processing and CO 2 separation; How far should the processing/refinement of biofuels go; Importance of factors of scale; New distributed (small-scale) energy technology; International trade in biofuels; Transport and handling costs for biofuel pellets in Europe; System aspects of implementation and incentives; How are biofuels affected if CO 2 from fossil fuels can be separated and

  6. Casein kinases

    DEFF Research Database (Denmark)

    Issinger, O G

    1993-01-01

    The present review on casein kinases focuses mainly on the possible metabolic role of CK-2, with special emphasis on its behavior in pathological tissues. From these data at least three ways to regulate CK-2 activity emerge: (i) CK-2 activity changes during embryogenesis, being high at certain...

  7. Successful Management of Crizotinib-Induced Neutropenia in a Patient with Anaplastic Lymphoma Kinase-Positive Non-Small Cell Lung Cancer: A Case Report

    Directory of Open Access Journals (Sweden)

    Jun Osugi

    2016-01-01

    Full Text Available Crizotinib, the first clinically available inhibitor of anaplastic lymphoma kinase (ALK gene rearrangement, is generally well tolerated. In contrast, neutropenia induced by crizotinib is a commonly reported grade 3 or 4 adverse event. In such cases, interruption and dose reduction of crizotinib might be necessary for some patients with severe neutropenia. However, information concerning clinical experience and management of severe neutropenia is currently limited. In this report, the successful management of crizotinib-induced neutropenia by dose reduction of crizotinib in a patient with ALK-positive non-small cell lung cancer is described.

  8. Kinases and Cancer

    OpenAIRE

    Jonas Cicenas; Egle Zalyte; Amos Bairoch; Pascale Gaudet

    2018-01-01

    Protein kinases are a large family of enzymes catalyzing protein phosphorylation. The human genome contains 518 protein kinase genes, 478 of which belong to the classical protein kinase family and 40 are atypical protein kinases [...

  9. The role of brain magnetic resonance imaging in the diagnosis of pantothenate kinase deficiency (previously Hallervorden-Spatz disease): case report

    International Nuclear Information System (INIS)

    Gizewska, M.; Bieleninik, A.; Adamczyk, T.; Walczak, M.; Cyrylowski, L.; Abramczyk, G.; Nowacki, P.

    2005-01-01

    Panthotenate kinase deficiency is a rare metabolic disorder from the group of neuroaxonal dystrophy. It is characterized by symptoms of extrapiramidal system impairment, general dystonia, progressive gait disturbances, limbs rigidity, dyskinesias, choreoatetotic movements, dysarthria and progressive dementia. On the brain MRI, T2 - weighted images demonstrate typical, symmetrical '' eye of the tiger '' sign with hyperintensive signals from the central parts of the pallidum, surrounded by low signals in the pallidum and accompanied by hypointensity in substantia nigra resulting from iron deposition. We present a case of 13.5 year old boy with gait disturbances, increase muscles tone, dysartria, aggressive behavior and learning difficulties progressing from the early childhood. In the differential diagnosis a number of inborn errors of metabolism was excluded. Finding on the brain MRI in T2- weighted images typical picture of '' eye of the tiger '' led to a diagnosis of panthotenate kinase deficiency. In subsequent years of observation, despite attempts of baclofen and calcium panthotenate treatment, progression of pyramidal-extrapyramidal syndrome is observed. In children with symptoms of progressive extrapyramidal tract impairment, a sign of '' eye of the tiger '' with hyperintensive signals from the central parts of the pallidum, surrounded by low signals in the remaining part of the pallidum and accompanied by similar lesions in substantia nigra is typical for panthotenate kinase deficiency - a rare neurodegenerative disorder of central nervous system. (author)

  10. Functional analysis of Casein Kinase 1 in a minimal circadian system.

    Directory of Open Access Journals (Sweden)

    Gerben van Ooijen

    Full Text Available The Earth's rotation has driven the evolution of cellular circadian clocks to facilitate anticipation of the solar cycle. Some evidence for timekeeping mechanism conserved from early unicellular life through to modern organisms was recently identified, but the components of this oscillator are currently unknown. Although very few clock components appear to be shared across higher species, Casein Kinase 1 (CK1 is known to affect timekeeping across metazoans and fungi, but has not previously been implicated in the circadian clock in the plant kingdom. We now show that modulation of CK1 function lengthens circadian rhythms in Ostreococcustauri, a unicellular marine algal species at the base of the green lineage, separated from humans by ~1.5 billion years of evolution. CK1 contributes to timekeeping in a phase-dependent manner, indicating clock-mediated gating of CK1 activity. Label-free proteomic analyses upon overexpression as well as inhibition revealed CK1-responsive phosphorylation events on a set of target proteins, including highly conserved potentially clock-relevant cellular regulator proteins. These results have major implications for our understanding of cellular timekeeping and can inform future studies in any circadian organism.

  11. Systems analysis department annual progress report 1986

    International Nuclear Information System (INIS)

    Grohnheit, P.E.; Larsen, H.; Vestergaard, N.K.

    1987-02-01

    The report describes the work of the Systems Analysis Department at Risoe National Laboratory during 1986. The activities may be classified as energy systems analysis and risk and reliability analysis. The report includes a list of staff members. (author)

  12. National Fire Incident Reporting System (NFIRS)

    Data.gov (United States)

    Department of Homeland Security — The National Fire Incident Reporting System (NFIRS) is a reporting standard that fire departments use to uniformly report on the full range of their activities, from...

  13. From Phosphosites to Kinases

    DEFF Research Database (Denmark)

    Munk, Stephanie; Refsgaard, Jan C; Olsen, Jesper V

    2016-01-01

    Kinases play a pivotal role in propagating the phosphorylation-mediated signaling networks in living cells. With the overwhelming quantities of phosphoproteomics data being generated, the number of identified phosphorylation sites (phosphosites) is ever increasing. Often, proteomics investigations...... sequence motifs, mostly based on large scale in vivo and in vitro experiments. The context of the kinase and the phosphorylated proteins in a biological system is equally important for predicting association between the enzymes and substrates, an aspect that is also being tackled with available...

  14. Thymidine kinase 2 and alanyl-tRNA synthetase 2 deficiencies cause lethal mitochondrial cardiomyopathy: case reports and review of the literature.

    Science.gov (United States)

    Mazurova, Stella; Magner, Martin; Kucerova-Vidrova, Vendula; Vondrackova, Alzbeta; Stranecky, Viktor; Pristoupilova, Anna; Zamecnik, Josef; Hansikova, Hana; Zeman, Jiri; Tesarova, Marketa; Honzik, Tomas

    2017-07-01

    Cardiomyopathy is a common manifestation in neonates and infants with mitochondrial disorders. In this study, we report two cases manifesting with fatal mitochondrial hypertrophic cardiomyopathy, which include the third known patient with thymidine kinase 2 deficiency and the ninth patient with alanyl-tRNA synthetase 2 deficiency. The girl with thymidine kinase 2 deficiency had hypertrophic cardiomyopathy together with regression of gross motor development at the age of 13 months. Neurological symptoms and cardiac involvement progressed into severe myopathy, psychomotor arrest, and cardiorespiratory failure at the age of 22 months. The imaging methods and autoptic studies proved that she suffered from unique findings of leucoencephalopathy, severe, mainly cerebellar neuronal degeneration, and hepatic steatosis. The girl with alanyl-tRNA synthetase 2 deficiency presented with cardiac failure and underlying hypertrophic cardiomyopathy within 12 hours of life and subsequently died at 9 weeks of age. Muscle biopsy analyses demonstrated respiratory chain complex I and IV deficiencies, and histological evaluation revealed massive mitochondrial accumulation and cytochrome c oxidase-negative fibres in both cases. Exome sequencing in the first case revealed compound heterozygozity for one novel c.209T>C and one previously published c.416C>T mutation in the TK2 gene, whereas in the second case homozygozity for the previously described mutation c.1774C>T in the AARS2 gene was determined. The thymidine kinase 2 mutations resulted in severe mitochondrial DNA depletion (to 12% of controls) in the muscle. We present, for the first time, severe leucoencephalopathy and hepatic steatosis in a patient with thymidine kinase 2 deficiency and the finding of a ragged red fibre-like image in the muscle biopsy in a patient with alanyl-tRNA synthetase 2 deficiency.

  15. AKDNR - DNR Business Reporting System (DBRS)

    Science.gov (United States)

    Skip to content State of Alaska myAlaska My Government Resident Business in Alaska Visiting Alaska Resources > IRM GPU > Main Menu DNR Business Reporting System (DBRS) The DNR Business Reporting System (DBRS) allows users to generate reports from the DNR Business databases and maps. The reports offered

  16. Airport Economics: Management Control Financial Reporting Systems

    Science.gov (United States)

    Buchbinder, A.

    1972-01-01

    The development of management control financial reporting systems for airport operation is discussed. The operation of the system to provide the reports required for determining the specific revenue producing facilities of airports is described. The organization of the cost reporting centers to show the types of information provided by the system is analyzed.

  17. Selective decrease of components of the creatine kinase system and ATP synthase complex in chronic Chagas disease cardiomyopathy.

    Directory of Open Access Journals (Sweden)

    Priscila Camillo Teixeira

    2011-06-01

    Full Text Available BACKGROUND: Chronic Chagas disease cardiomyopathy (CCC is an inflammatory dilated cardiomyopathy with a worse prognosis than other cardiomyopathies. CCC occurs in 30 % of individuals infected with Trypanosoma cruzi, endemic in Latin America. Heart failure is associated with impaired energy metabolism, which may be correlated to contractile dysfunction. We thus analyzed the myocardial gene and protein expression, as well as activity, of key mitochondrial enzymes related to ATP production, in myocardial samples of end-stage CCC, idiopathic dilated (IDC and ischemic (IC cardiomyopathies. METHODOLOGY/PRINCIPAL FINDINGS: Myocardium homogenates from CCC (N=5, IC (N=5 and IDC (N=5 patients, as well as from heart donors (N=5 were analyzed for protein and mRNA expression of mitochondrial creatine kinase (CKMit and muscular creatine kinase (CKM and ATP synthase subunits aplha and beta by immunoblotting and by real-time RT-PCR. Total myocardial CK activity was also assessed. Protein levels of CKM and CK activity were reduced in all three cardiomyopathy groups. However, total CK activity, as well as ATP synthase alpha chain protein levels, were significantly lower in CCC samples than IC and IDC samples. CCC myocardium displayed selective reduction of protein levels and activity of enzymes crucial for maintaining cytoplasmic ATP levels. CONCLUSIONS/SIGNIFICANCE: The selective impairment of the CK system may be associated to the loss of inotropic reserve observed in CCC. Reduction of ATP synthase alpha levels is consistent with a decrease in myocardial ATP generation through oxidative phosphorylation. Together, these results suggest that the energetic deficit is more intense in the myocardium of CCC patients than in the other tested dilated cardiomyopathies.

  18. Very late-onset group B Streptococcus meningitis, sepsis, and systemic shigellosis due to interleukin-1 receptor-associated kinase-4 deficiency.

    Science.gov (United States)

    Krause, Jens C; Ghandil, Pegah; Chrabieh, Maya; Casanova, Jean-Laurent; Picard, Capucine; Puel, Anne; Creech, C Buddy

    2009-11-01

    We describe a child with very late-onset group B Streptococcus sepsis and meningitis, systemic shigellosis, and chronic osteomyelitis. Peripheral blood cells obtained from the patient and her brother did not respond to stimulation with either interleukin-1beta or lipopolysaccharide. Sequencing of the interleukin-1 receptor-associated kinase-4 gene revealed 2 novel mutations.

  19. Tank waste remediation system mission analysis report

    International Nuclear Information System (INIS)

    Acree, C.D.

    1998-01-01

    The Tank Waste Remediation System Mission Analysis Report identifies the initial states of the system and the desired final states of the system. The Mission Analysis Report identifies target measures of success appropriate to program-level accomplishments. It also identifies program-level requirements and major system boundaries and interfaces

  20. Pyruvate kinase M2 overexpression and poor prognosis in solid tumors of digestive system: evidence from 16 cohort studies.

    Science.gov (United States)

    Wu, Jiayuan; Hu, Liren; Chen, Manyu; Cao, Wenjun; Chen, Haicong; He, Taiping

    2016-01-01

    The expression of pyruvate kinase M2 (PKM2) has been linked to tumor formation and invasion. Specifically, the relationship between high PKM2 expression and prognosis has been evaluated in solid tumors of digestive system. However, the prognostic value of PKM2 remains controversial. A literature search of PubMed, Embase, and Cochrane databases was conducted until October 2015. The end point focused on overall survival (OS). The pooled hazard ratio (HR) or odds ratio and the 95% confidence intervals were calculated to correlate PKM2 overexpression with OS and clinicopathological characteristics by employing fixed- or random-effects models, depending on the heterogeneity of the included studies. We identified 18 cohorts in 16 studies involving 2,812 patients for this meta-analysis. Overall, the combined HR for OS in all tumor types was 1.74 (1.44-2.11; Pdigestive system, thereby suggesting that PKM2 might be an indicator of poor prognosis in digestive system cancers.

  1. A proteomic approach for comprehensively screening substrates of protein kinases such as Rho-kinase.

    Directory of Open Access Journals (Sweden)

    Mutsuki Amano

    Full Text Available BACKGROUND: Protein kinases are major components of signal transduction pathways in multiple cellular processes. Kinases directly interact with and phosphorylate downstream substrates, thus modulating their functions. Despite the importance of identifying substrates in order to more fully understand the signaling network of respective kinases, efficient methods to search for substrates remain poorly explored. METHODOLOGY/PRINCIPAL FINDINGS: We combined mass spectrometry and affinity column chromatography of the catalytic domain of protein kinases to screen potential substrates. Using the active catalytic fragment of Rho-kinase/ROCK/ROK as the model bait, we obtained about 300 interacting proteins from the rat brain cytosol fraction, which included the proteins previously reported as Rho-kinase substrates. Several novel interacting proteins, including doublecortin, were phosphorylated by Rho-kinase both in vitro and in vivo. CONCLUSIONS/SIGNIFICANCE: This method would enable identification of novel specific substrates for kinases such as Rho-kinase with high sensitivity.

  2. A kinase-dead knock-in mutation in mTOR leads to early embryonic lethality and is dispensable for the immune system in heterozygous mice

    Directory of Open Access Journals (Sweden)

    Cavender Druie

    2009-05-01

    Full Text Available Abstract Background The mammalian target of rapamycin protein (mTOR is an evolutionarily conserved kinase that regulates protein synthesis, cell cycle progression and proliferation in response to various environmental cues. As a critical downstream mediator of PI3K signaling, mTOR is important for lymphocyte development and function of mature T and B-cells. Most studies of mTOR in immune responses have relied on the use of pharmacological inhibitors, such as rapamycin. Rapamycin-FKBP12 complex exerts its immunosuppressive and anti-proliferative effect by binding outside the kinase domain of mTOR, and subsequently inhibiting downstream mTOR signaling. Results To determine the requirement for mTOR kinase activity in the immune system function, we generated knock-in mice carrying a mutation (D2338 in the catalytic domain of mTOR. While homozygous mTOR kd/kd embryos died before embryonic day 6.5, heterozygous mTOR+/kd mice appeared entirely normal and are fertile. mTOR +/kd mice exhibited normal T and B cell development and unaltered proliferative responses of splenocytes to IL-2 and TCR/CD28. In addition, heterozygousity for the mTOR kinase-dead allele did not sensitize T cells to rapamycin in a CD3-mediated proliferation assay. Unexpectedly, mTOR kinase activity towards its substrate 4E-BP1 was not decreased in hearts and livers from heterozygous animals. Conclusion Altogether, our findings indicate that mTOR kinase activity is indispensable for the early development of mouse embryos. Moreover, a single wild type mTOR allele is sufficient to maintain normal postnatal growth and lymphocyte development and proliferation.

  3. Northeast Electronic Reporting System (NERS)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NOAA/NEFSC Study fleets are a subset of fishing vessels from which high quality, self-reported data on fishing effort, area fished, gear characteristics, catch,...

  4. NONLINEAR DYNAMICAL SYSTEMS - Final report

    Energy Technology Data Exchange (ETDEWEB)

    Philip Holmes

    2005-12-31

    This document is the final report on the work completed on DE-FG02-95ER25238 since the start of the second renewal period: Jan 1, 2001. It supplements the annual reports submitted in 2001 and 2002. In the renewal proposal I envisaged work in three main areas: Analytical and topological tools for studying flows and maps Low dimensional models of fluid flow Models of animal locomotion and I describe the progess made on each project.

  5. Ribosomal S6 Kinase Cooperates with Casein Kinase 2 to Modulate the Drosophila Circadian Molecular Oscillator

    Science.gov (United States)

    Akten, Bikem; Tangredi, Michelle M.; Jauch, Eike; Roberts, Mary A.; Ng, Fanny; Raabe, Thomas; Jackson, F. Rob

    2009-01-01

    There is a universal requirement for post-translational regulatory mechanisms in circadian clock systems. Previous work in Drosophila has identified several kinases, phosphatases and an E3 ligase that are critical for determining the nuclear translocation and/or stability of clock proteins. The present study evaluated the function of p90 ribosomal S6 kinase (RSK) in the Drosophila circadian system. In mammals, RSK1 is a light- and clock-regulated kinase known to be activated by the MAPK pathway, but there is no direct evidence that it functions as a component of the circadian system. Here, we show that Drosophila S6KII RNA displays rhythms in abundance, indicative of circadian control. Importantly, an S6KII null mutant exhibits a short-period circadian phenotype that can be rescued by expression of the wild-type gene in clock neurons, indicating a role for S6KII in the molecular oscillator. Peak PER clock protein expression is elevated in the mutant, indicative of enhanced stability, whereas per mRNA level is decreased, consistent with enhanced feedback repression. Gene reporter assays show that decreased S6KII is associated with increased PER repression. Surprisingly, we demonstrate a physical interaction between S6KII and the Casein Kinase 2 regulatory subunit (CK2β), suggesting a functional relationship between the two kinases. In support of such a relationship, there are genetic interactions between S6KII and CK2 mutations, in vivo, which indicate that CK2 activity is required for S6KII action. We propose that the two kinases cooperate within clock neurons to fine-tune circadian period, improving the precision of the clock mechanism. PMID:19144847

  6. Critical Incident Reporting Systems: Perceived Competing Social ...

    African Journals Online (AJOL)

    The safe operation of complex socio-technical systems is dependent upon the reporting of safety critical incidents by operators within a system. Through the action of reporting, systems develop the capability as a learning organisation to improve human and organisational performance. The aim of the study is therefore to ...

  7. Successful oral desensitization against skin rash induced by alectinib in a patient with anaplastic lymphoma kinase-positive lung adenocarcinoma: A case report.

    Science.gov (United States)

    Shirasawa, Masayuki; Kubotaa, Masaru; Harada, Shinya; Niwa, Hideyuki; Kusuhara, Seiichiro; Kasajima, Masashi; Hiyoshi, Yasuhiro; Ishihara, Mikiko; Igawa, Satoshi; Masuda, Noriyuki

    2016-09-01

    Alectinib has been approved for the treatment of patients with anaplastic lymphoma kinase (ALK) gene rearrangement-positive advanced non-small cell lung cancer. In terms of adverse effects, the occurrence of a severe skin rash induced by alectinib is reportedly rare, compared with the occurrence of skin rash induced by epithelial growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs). In the present case report, a 76-year-old woman with ALK-positive lung adenocarcinoma experienced disease progression after undergoing first-line chemotherapy. Subsequently, alectinib was administered as a second-line therapy. However, she discontinued alectinib therapy after 11days because of the occurrence of an alectinib-induced skin rash. Since the skin rash improved within one week, we attempted to perform oral desensitization to alectinib. The patient has not shown any recurrence of the rash or disease progression for 7 months since the successful oral desensitization to alectinib. Here, we describe the first case of successful oral desensitization against a skin rash induced by alectinib in a patient with ALK-positive lung adenocarcinoma. Desensitization to overcome adverse effects and to enable sustained treatment with alectinib should be considered in patients who develop alectinib sensitivities. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  8. Medicares Physician Quality Reporting System (PQRS)...

    Data.gov (United States)

    U.S. Department of Health & Human Services — Medicares Physician Quality Reporting System (PQRS) allows providers to report measures of process quality and health outcomes. The authors of Medicares Physician...

  9. CDC Wonder Vaccine Adverse Event Reporting System

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Vaccine Adverse Event Reporting System (VAERS) online database on CDC WONDER provides counts and percentages of adverse event case reports after vaccination,...

  10. Electronic Resources Management System: Recommendation Report 2017

    KAUST Repository

    Ramli, Rindra M.

    2017-01-01

    This recommendation report provides an overview of the selection process for the new Electronic Resources Management System. The library has decided to move away from Innovative Interfaces Millennium ERM module. The library reviewed 3 system

  11. Systemic inhibition of mTOR kinase via rapamycin disrupts consolidation and reconsolidation of auditory fear memory.

    Science.gov (United States)

    Mac Callum, Phillip E; Hebert, Mark; Adamec, Robert E; Blundell, Jacqueline

    2014-07-01

    The mammalian target of rapamycin (mTOR) kinase is a critical regulator of mRNA translation and is known to be involved in various long lasting forms of synaptic and behavioural plasticity. However, information concerning the temporal pattern of mTOR activation and susceptibility to pharmacological intervention during both consolidation and reconsolidation of long-term memory (LTM) remains scant. Male C57BL/6 mice were injected systemically with rapamycin at various time points following conditioning or retrieval in an auditory fear conditioning paradigm, and compared to vehicle (and/or anisomycin) controls for subsequent memory recall. Systemic blockade of mTOR with rapamycin immediately or 12h after training or reactivation impairs both consolidation and reconsolidation of an auditory fear memory. Further behavioural analysis revealed that the enduring effects of rapamycin on reconsolidation are dependent upon reactivation of the memory trace. Rapamycin, however, has no effect on short-term memory or the ability to retrieve an established fear memory. Collectively, our data suggest that biphasic mTOR signalling is essential for both consolidation and reconsolidation-like activities that contribute to the formation, re-stabilization, and persistence of long term auditory-fear memories, while not influencing other aspects of the memory trace. These findings also provide evidence for a cogent treatment model for reducing the emotional strength of established, traumatic memories analogous to those observed in acquired anxiety disorders such as posttraumatic stress disorder (PTSD) and specific phobias, through pharmacologic blockade of mTOR using systemic rapamycin following reactivation. Copyright © 2013 Elsevier Inc. All rights reserved.

  12. Apollo experience report: Food systems

    Science.gov (United States)

    Smith, M. C., Jr.; Rapp, R. M.; Huber, C. S.; Rambaut, P. C.; Heidelbaugh, N. D.

    1974-01-01

    Development, delivery, and use of food systems in support of the Apollo 7 to 14 missions are discussed. Changes in design criteria for this unique program as mission requirements varied are traced from the baseline system that was established before the completion of the Gemini Program. Problems and progress in subsystem management, material selection, food packaging, development of new food items, menu design, and food-consumption methods under zero-gravity conditions are described. The effectiveness of various approaches in meeting food system objectives of providing flight crews with safe, nutritious, easy to prepare, and highly acceptable foods is considered. Nutritional quality and adequacy in maintaining crew health are discussed in relation to the establishment of nutritional criteria for future missions. Technological advances that have resulted from the design of separate food systems for the command module, the lunar module, The Mobile Quarantine Facility, and the Lunar Receiving Laboratory are presented for application to future manned spacecraft and to unique populations in earthbound situations.

  13. Preoperational test report, primary ventilation system

    International Nuclear Information System (INIS)

    Clifton, F.T.

    1997-01-01

    This represents a preoperational test report for Primary Ventilation Systems, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system provides vapor space filtered venting of tanks AY101, AY102, AZ101, AZ102. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System

  14. Preoperational test report, primary ventilation system

    Energy Technology Data Exchange (ETDEWEB)

    Clifton, F.T.

    1997-11-04

    This represents a preoperational test report for Primary Ventilation Systems, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system provides vapor space filtered venting of tanks AY101, AY102, AZ101, AZ102. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System.

  15. Preoperational test report, vent building ventilation system

    International Nuclear Information System (INIS)

    Clifton, F.T.

    1997-01-01

    This represents a preoperational test report for Vent Building Ventilation Systems, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system provides Heating, Ventilation, and Air Conditioning (HVAC) for the W-030 Ventilation Building. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System

  16. Tomato thymidine kinase is subject to inefficient TTP feedback regulation

    DEFF Research Database (Denmark)

    Larsen, Nicolai Balle; Munch-Petersen, Birgitte; Piskur, Jure

    2014-01-01

    A promising suicide gene therapy system to treat gliomas has been reported: the thymidine kinase 1 from tomato (toTK1) combined with the nucleoside analog pro-drug zidovudine (azidothymidine, AZT), which is known to penetrate the blood–brain barrier. Transduction with toTK1 has been found to effi...

  17. Case report 561: Systemic mastocytosis

    Energy Technology Data Exchange (ETDEWEB)

    Schweitzer, M.E.; Irwin, G.A.L. (Nassau County Medical Center, East Meadow, NY (USA). Dept. of Radiology)

    1989-08-01

    A case is presented of a 55-year-old man with systemic mastocytosis. CT studies showed mesenteric and retroperetoneal lymphadenopathy, hepatosplenomegaly and sclerotic lesions of lumbar vertebrae. Lesions of the skin were absent. Pathological studies of lymph nodes indicated the presence of mastocytosis. The clinical, radiological and pathological features of this disorder and its five forms were discussed. Prognosis and treatment were also considered. (orig./GDG).

  18. Case report 369: Systemic mastocytosis

    Energy Technology Data Exchange (ETDEWEB)

    Rodenberg, J.C.; Maegaard, K.K.; Svanholm, H.

    1986-05-01

    In summary, the case of a 58-year-old woman with upper gastrointestinal symptoms and a reddish-brown skin rash over a number of years has been presented. Biopsies from various sites, including bone marrow, were negative initially. A repeat cutaneous biopsy demonstrated the presence of systemic mastocytis. The characteristic clinical and radiological aspects of mastocytosis were described and the pathological features also were considered. A relevant differential diagnosis of the sclerotic form of mastocytosis was presented.

  19. Systems Analysis Department annual progress report 1998

    DEFF Research Database (Denmark)

    1999-01-01

    The report describes the work of the Systems Analysis Department at Risø National Laboratory during 1998. The department undertakes research within Energy Systems Analysis, Integrated Energy, Environment and Development Planning - UNEP Centre, IndustrialSafety and Reliability, Man/Machine Interac....../Machine Interaction, and Technology Scenarios. The report includes lists of publications, lectures, committees and staff members....

  20. Systems Analysis Department. Annual Report 2003

    Energy Technology Data Exchange (ETDEWEB)

    Larsen, H.; Olsson, C. (eds.)

    2004-04-01

    This report describes the work of the Systems Analysis Department at Risoe National Laboratory during 2003. The department is undertaking research within Energy Systems Analysis, Energy, Environment and Development Planning UNEP Centre, Safety, Reliability and Human Factors, and Technology Scenarios. The report includes summary statistics and list of staff members. (au)

  1. Systems Analysis Department. Annual Report 2001

    Energy Technology Data Exchange (ETDEWEB)

    Duijm, N J; Jensen, E; Larsen, H; Skipper, S [eds.

    2002-04-01

    This report describes the work of the Systems Analysis Department at Risoe National Laboratory during 2001. The department is undertaking research within Energy Systems Analysis, Energy, Environment and Development Planning - UNEP Centre, Safety, Reliability and Human Factors, and Technology Scenarios. The report includes summary statistics and lists of publications, committees and staff members. (au)

  2. Systems Analysis Department annual report 2003

    DEFF Research Database (Denmark)

    2004-01-01

    This report describes the work of the Systems Analysis Department at Risø National Laboratory during 2003. The department is undertaking research within Energy Systems Analysis, Energy, Environment and Development Planning – UNEP Centre, Safety,Reliability and Human Factors, and Technology...... Scenarios. The report includes summary statistics and list of staff members....

  3. Systems Analysis Department. Annual Progress Report 1999

    Energy Technology Data Exchange (ETDEWEB)

    Larsen, Hans; Olsson, Charlotte; Loevborg, Leif [eds.

    2000-03-01

    This report describes the work of the Systems Analysis Department at Risoe National Laboratory during 1999. The department is undertaking research within Energy Systems Analysis, Energy, Environment and Development Planning-UNEP Centre, Safety, Reliability and Human Factors, and Technology Scenarios. The report includes summary statistics and lists of publications, committees and staff members. (au)

  4. Systems Analysis department. Annual progress report 1997

    Energy Technology Data Exchange (ETDEWEB)

    Larsen, Hans; Olsson, Charlotte; Petersen, Kurt E

    1998-03-01

    The report describes the work of the Systems Analysis Department at Risoe National Laboratory during 1997. The department is undertaking research within Energy systems Analysis, Integrated Energy, Environment and Development Planning - UNEP Centre, Industrial Safety and Reliability and Man/Machine Interaction. The report includes lists of publications lectures, committees and staff members. (au) 110 refs.

  5. Systems Analysis Department. Annual progress report 1996

    Energy Technology Data Exchange (ETDEWEB)

    Larsen, H; Olsson, C; Petersen, K E [eds.

    1997-03-01

    The report describes the work of the Systems Analysis Department at Risoe National Laboratory during 1996. The department is undertaking research within Simulation and Optimisation of Energy Systems, Energy and Environment in Developing Countries - UNEP Centre, Integrated Environmental and Risk Management and Man/Machine Interaction. The report includes lists of publications, lectures, committees and staff members. (au) 131 refs.

  6. Energy Systems Group. Annual Progress Report 1984

    DEFF Research Database (Denmark)

    Grohnheit, Poul Erik; Larsen, Hans Hvidtfeldt; Villadsen, B.

    The report describes the work of the Energy Systems Group at Risø National Laboratory during 1984. The activities may be roughly classified as development and use of energy-economy models, energy systems analysis, energy technology assessment and energy planning. The report includes a list of staff...

  7. System Analysis Department. Annual Report 2002

    Energy Technology Data Exchange (ETDEWEB)

    Duijm, N J; Jensen, E; Larsen, H; Skipper, S [eds.

    2002-04-01

    This report describes the work of the Systems Analysis Department at Risoe National Laboratory during 2001. The department is undertaking research within Energy Systems Analysis, Energy, Environment and Development Planning - UNEP Centre, Safety, Reliability and Human Factors, and Technology Scenarios. The report includes summary statistics and lists of publications, committees and staff members. (au)

  8. Systems Analysis Department. Annual Report 2000

    Energy Technology Data Exchange (ETDEWEB)

    Duijm, N J; Jensen, E; Larsen, H; Olsson, C

    2001-05-01

    This report describes the work of the Systems Analysis Department at Risoe National Laboratory during 2000. The department is undertaking research within Energy Systems Analysis, Energy, Environment and Development Planning - UNEP Centre, Safety, Reliability and Human Factors, and Technology Scenarios. The report includes summary statistics and lists of publications, committees and staff members. (au)

  9. Systems Analysis Department annual progress report 1998

    Energy Technology Data Exchange (ETDEWEB)

    Larsen, Hans; Olsson, Charlotte; Loevborg, Leif [eds.

    1999-03-01

    The report describes the work of the Systems Analysis Department at Risoe National Laboratory during 1998. The department undertakes research within Energy Systems Analysis, Integrated Energy, Environment and Development Planning - UNEP Centre, Industrial Safety and Reliability, Man/Machine Interaction and Technology Scenarios. The report includes lists of publications, lectures, committees and staff members. (au) 111 refs.

  10. Energy Systems Group annual progress report 1984

    International Nuclear Information System (INIS)

    Grohnheit, P.E.; Larsen, H.; Villadsen, B.

    1985-02-01

    The report describes the work of the Energy Systems Group at Risoe National Laboratory during 1984. The activities may be roughly classified as development and use of energy-economy models, energy systems analysis, energy technology assessment and energy planning. The report includes a list of staff members. (author)

  11. Medication incidents reported to an online incident reporting system.

    LENUS (Irish Health Repository)

    Alrwisan, Adel

    2011-01-15

    AIMS: Approximately 20% of deaths from adverse events are related to medication incidents, costing the NHS an additional £500 million annually. Less than 5% of adverse events are reported. This study aims to assess the reporting rate of medication incidents in NHS facilities in the north east of Scotland, and to describe the types and outcomes of reported incidents among different services. Furthermore, we wished to quantify the proportion of reported incidents according to the reporters\\' profession. METHODS: A retrospective description was made of medication incidents reported to an online reporting system (DATIX) over a 46-month-period (July 2005 to April 2009). Reports originated from acute and community hospitals, mental health, and primary care facilities. RESULTS: Over the study period there were 2,666 incidents reported with a mean monthly reporting rate of 78.2\\/month (SD±16.9). 6.1% of all incidents resulted in harm, with insulin being the most commonly implicated medication. Nearly three-quarters (74.2%, n=1,978) of total incidents originated from acute hospitals. Administration incidents were implicated in the majority of the reported medication incidents (59%), followed by prescribing (10.8%) and dispensing (9.9%), while the nondescript "other medication incidents" accounted for 20.3% of total incidents. The majority of reports were made by nursing and midwifery staff (80%), with medical and dental professionals reporting the lowest number of incidents (n=56, 2%). CONCLUSIONS: The majority of medication incidents in this study were reported by nursing and midwifery staff, and were due to administration incidents. There is a clear need to elucidate the reasons for the limited contribution of the medical and dental professionals to reporting medication incidents.

  12. NNWSI project information management system concepts evaluation report. Final report

    International Nuclear Information System (INIS)

    1986-08-01

    This report is intended as a first step in developing detailed information management system specifications for the Nevada Nuclear Waste Storage Investigations (NNWSI) Project. The current state of information management at the NNWSI Project level is investigated and an information management system (IMS) is proposed. The IMS as it relates to aspects of Project and records management is discussed. Information management concepts and prospective IMS system components are investigated. Concepts and system components include: indexing, searching, retrieval, data base management system technology, computers, storage media, computer-assisted retrieval (CAR) of microfilm, electronic imaging-based systems, optical character recognition, and communications. Performance criteria and desirable system attributes applicable to the IMS are discussed. Six conceptual system approaches capable of satisfying the performance criteria are defined. System approaches include: fully centralized microfilm system based on CAR retrieval (Approach 1), partially distributed microfilm system based on CAR retrieval (Approach 2), fully distributed microfilm system based on CAR retrieval (Approach 3), fully centralized optical disk system based on electronic image and full-text retrieval (Approach 4), partially distributed optical system based on electron image and full-text retrieval (Approach 5), and fully distributed optical disk system based on electronic image and full-text retrieval (Approach 6). Technical and cost considerations associated with the six conceptual approaches are evaluated. Technical evaluation results indicate Approach 4 is the best conceptual approach, and cost evaluation results show no significant differences among approaches. On the basis of the evaluation, Approach 4 is recommended

  13. Pamela tracking system status report

    CERN Document Server

    Taccetti, F; Bonechi, L; Bongi, M; Boscherini, M; Castellini, G; D'Alessandro, R; Gabbanini, A; Grandi, M; Papini, P; Piccardi, S; Ricciarini, S; Spillantini, P; Straulino, S; Tesi, M; Vannuccini, E

    2002-01-01

    The Pamela apparatus will be launched at the end of 2002 on board of the Resurs DK Russian satellite. The tracking system, composed of six planes of silicon sensors inserted inside a permanent magnetic field was intensively tested during these last years. Results of tests have shown a good signal-to-noise ratio and an excellent spatial resolution, which should allow to measure the antiproton flux in an energy range from 80 MeV up to 190 GeV. The production of the final detector modules is about to start and mechanical and thermal tests on the tracking tower are being performed according to the specifications of the Russian launcher and satellite.

  14. Lunar power systems. Final report

    International Nuclear Information System (INIS)

    1986-12-01

    The findings of a study on the feasibility of several methods of providing electrical power for a permanently manned lunar base are provided. Two fundamentally different methods for lunar electrical power generation are considered. One is the use of a small nuclear reactor and the other is the conversion of solar energy to electricity. The baseline goal was to initially provide 300 kW of power with growth capability to one megawatt and eventually to 10 megawatts. A detailed, day by day scenario for the establishment, build-up, and operational activity of the lunar base is presented. Also presented is a conceptual approach to a supporting transportation system which identifies the number, type, and deployment of transportation vehicles required to support the base. An approach to the use of solar cells in the lunar environment was developed. There are a number of heat engines which are applicable to solar/electric conversions, and these are examined. Several approaches to energy storage which were used by the electric power utilities were examined and those which could be used at a lunar base were identified

  15. Creatine-creatine phosphate shuttle modeled as two-compartment system at different levels of creatine kinase activity

    DEFF Research Database (Denmark)

    Fedosov, Sergey

    1994-01-01

    In order to characterize ADP-ATP and creatine-creatine phosphate (Cr-CrP) shuttles a minimal mathematical model with two compartments and cyclic turnover of matter was designed. The 'mitochondrial' compartment contained 'ATP-synthase' and 'mitochondrial ereatine kinase' (mitCK). The 'cytoplasmic......' compartment consisted of 'ATPase', 'cytoplasmic creatine kinase' (cytCK) and an 'ADP-binding structure'. The exchange of metabolites between these compartments was limited. Different levels of cytCK and mitCK expression as welt as different exchange rate constants between the compartments were assigned...

  16. Energy Innovation Systems Indicator Report 2012

    DEFF Research Database (Denmark)

    Klitkou, Antje; Borup, Mads; Iversen, Eric

    This report is the first report in a series of reports on energy innovation system indicators produced as part of the activities in the “EIS Strategic research alliance for Energy Innovation Systems and their dynamics – Denmark in global competition”. The work is based on a number of existing......). The report received also valuable input from a project commissioned by IPTS. This project addressed co-operation patterns and knowledge flows in patent documents in the fields of wind energy, photovoltaic energy and concentrating solar power (Iversen and Patel, 2010). The results relevant for this project...

  17. Army Energy and Water Reporting System Assessment

    Energy Technology Data Exchange (ETDEWEB)

    Deprez, Peggy C.; Giardinelli, Michael J.; Burke, John S.; Connell, Linda M.

    2011-09-01

    There are many areas of desired improvement for the Army Energy and Water Reporting System. The purpose of system is to serve as a data repository for collecting information from energy managers, which is then compiled into an annual energy report. This document summarizes reported shortcomings of the system and provides several alternative approaches for improving application usability and adding functionality. The U.S. Army has been using Army Energy and Water Reporting System (AEWRS) for many years to collect and compile energy data from installations for facilitating compliance with Federal and Department of Defense energy management program reporting requirements. In this analysis, staff from Pacific Northwest National Laboratory found that substantial opportunities exist to expand AEWRS functions to better assist the Army to effectively manage energy programs. Army leadership must decide if it wants to invest in expanding AEWRS capabilities as a web-based, enterprise-wide tool for improving the Army Energy and Water Management Program or simply maintaining a bottom-up reporting tool. This report looks at both improving system functionality from an operational perspective and increasing user-friendliness, but also as a tool for potential improvements to increase program effectiveness. The authors of this report recommend focusing on making the system easier for energy managers to input accurate data as the top priority for improving AEWRS. The next major focus of improvement would be improved reporting. The AEWRS user interface is dated and not user friendly, and a new system is recommended. While there are relatively minor improvements that could be made to the existing system to make it easier to use, significant improvements will be achieved with a user-friendly interface, new architecture, and a design that permits scalability and reliability. An expanded data set would naturally have need of additional requirements gathering and a focus on integrating

  18. Engineering of kinase-based protein interacting devices: active expression of tyrosine kinase domains

    KAUST Repository

    Diaz Galicia, Miriam Escarlet

    2018-05-01

    Protein-protein interactions modulate cellular processes in health and disease. However, tracing weak or rare associations or dissociations of proteins is not a trivial task. Kinases are often regulated through interaction partners and, at the same time, themselves regulate cellular interaction networks. The use of kinase domains for creating a synthetic sensor device that reads low concentration protein-protein interactions and amplifies them to a higher concentration interaction which is then translated into a FRET (Fluorescence Resonance Energy Transfer) signal is here proposed. To this end, DNA constructs for interaction amplification (split kinases), positive controls (intact kinase domains), scaffolding proteins and phosphopeptide - SH2-domain modules for the reading of kinase activity were assembled and expression protocols for fusion proteins containing Lyn, Src, and Fak kinase domains in bacterial and in cell-free systems were optimized. Also, two non-overlapping methods for measuring the kinase activity of these proteins were stablished and, finally, a protein-fragment complementation assay with the split-kinase constructs was tested. In conclusion, it has been demonstrated that features such as codon optimization, vector design and expression conditions have an impact on the expression yield and activity of kinase-based proteins. Furthermore, it has been found that the defined PURE cell-free system is insufficient for the active expression of catalytic kinase domains. In contrast, the bacterial co-expression with phosphatases produced active kinase fusion proteins for two out of the three tested Tyrosine kinase domains.

  19. The NASA Aviation Safety Reporting System

    Science.gov (United States)

    1983-01-01

    This is the fourteenth in a series of reports based on safety-related incidents submitted to the NASA Aviation Safety Reporting System by pilots, controllers, and, occasionally, other participants in the National Aviation System (refs. 1-13). ASRS operates under a memorandum of agreement between the National Aviation and Space Administration and the Federal Aviation Administration. The report contains, first, a special study prepared by the ASRS Office Staff, of pilot- and controller-submitted reports related to the perceived operation of the ATC system since the 1981 walkout of the controllers' labor organization. Next is a research paper analyzing incidents occurring while single-pilot crews were conducting IFR flights. A third section presents a selection of Alert Bulletins issued by ASRS, with the responses they have elicited from FAA and others concerned. Finally, the report contains a list of publications produced by ASRS with instructions for obtaining them.

  20. Intelligent Transportation Systems statewide architecture : final report.

    Science.gov (United States)

    2003-06-01

    This report describes the development of Kentuckys Statewide Intelligent Transportation Systems (ITS) Architecture. The process began with the development of an ITS Strategic Plan in 1997-2000. A Business Plan, developed in 2000-2001, translated t...

  1. Traffic Management Systems Performance Measurement: Final Report

    OpenAIRE

    Banks, James H.; Kelly, Gregory

    1997-01-01

    This report documents a study of performance measurement for Transportation Management Centers (TMCs). Performance measurement requirements were analyzed, data collection and management techniques were investigated, and case study traffic data system improvement plans were prepared for two Caltrans districts.

  2. Grants Reporting and Tracking System (GRTS)

    Data.gov (United States)

    U.S. Environmental Protection Agency — The Grants Reporting and Tracking System (GRTS) is the primary tool for management and oversight of EPA's Nonpoint Source (NPS) Pollution Control Program. GRTS pulls...

  3. National Violent Death Reporting System (NVDRS)

    Data.gov (United States)

    U.S. Department of Health & Human Services — The National Violent Death Reporting System (NVDRS) provides states and communities with a clearer understanding of violent deaths to guide local decisions about...

  4. Patient-reported adverse drug reactions and their influence on adherence and quality of life of chronic myeloid leukemia patients on per oral tyrosine kinase inhibitor treatment

    Directory of Open Access Journals (Sweden)

    Kekäle M

    2015-12-01

    Full Text Available Meri Kekäle,1 Marikki Peltoniemi,2 Marja Airaksinen1 1Clinical Pharmacy Group, Division of Pharmacology and Pharmacotherapy, 2Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland Purpose: To evaluate adverse drug reactions (ADRs experienced by chronic myeloid leukemia (CML patients during per oral tyrosine kinase inhibitor (TKI treatment and correlation of ADR symptoms with medication adherence and perceived quality of life (QoL.Patients and methods: Eighty-six adult, chronic-phase CML patients who had been on TKI treatment (79% on imatinib, 10.5% dasatinib, and 10.5% nilotinib for at least 6 months participated in the study (mean age: 57.8 years, 52% males. The mean time from diagnosis was 5.1 years. All patients were interviewed, and patient-reported ADRs were obtained using a structured list. Adherence was assessed using Morisky’s 8-item Medication Adherence Scale (MMAS. The symptoms’ interference with patient’s daily QoL was measured by asking patients about the influence of symptom(s on their mood, general condition, enjoyment of life, walking, relationships, and work.Results: Ninety-seven percent of the patients were suffering from at least one ADR. The mean number of different symptoms was seven (range: 0–15, median 6. The most commonly perceived ADRs were muscle soreness or cramp (69/86, 80%; swelling of hands, legs, feet, or around the eyes (59/86, 69%; and fatigue (43/86, 50%. No correlation was found between adherence and ADRs, because symptoms were equally common in each MMAS adherence class. Half of the patients felt that the ADRs had a negative influence on their daily QoL. A quarter of the patients reported that ADRs affected either their mood, general condition, or enjoyment of life. The incidence of almost all ADRs was much higher among patients reporting negative influence of ADRs on their daily life compared to total study population (P=0.016.Conclusion

  5. Preoperational test report, primary ventilation condensate system

    Energy Technology Data Exchange (ETDEWEB)

    Clifton, F.T.

    1997-01-29

    Preoperational test report for Primary Ventilation Condensate System, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system provides a collection point for condensate generated by the W-030 primary vent offgas cooling system serving tanks AYIOI, AY102, AZIOI, AZI02. The system is located inside a shielded ventilation equipment cell and consists of a condensate seal pot, sampling features, a drain line to existing Catch Tank 241-AZ-151, and a cell sump jet pump. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System.

  6. Traceability of biopharmaceuticals in spontaneous reporting systems

    DEFF Research Database (Denmark)

    Vermeer, Niels S; Straus, Sabine M J M; Mantel-Teeuwisse, Aukje K

    2013-01-01

    the period 2004-2010, including ADR reports from two major SRSs: the FDA Adverse Event Reporting System (FAERS) in the US and EudraVigilance (EV) in the EU. MAIN OUTCOME MEASURES: The availability of batch numbers was determined for biopharmaceuticals, and compared with small molecule drugs...

  7. The Retinal Readout System: a status report A Status Report

    CERN Document Server

    Litke, A M

    1999-01-01

    The 'Retinal Readout System' is being developed to study the language the eye uses to send information about the visual world to the brain. Its architecture is based on that of silicon microstrip detectors. An array of 512 microscopic electrodes picks up the signals generated by the output neurons of live retinal tissue in response to a dynamic image focused on the input neurons. These signals are amplified, filtered and multiplexed by a set of eight custom-designed VLSI readout chips, and digitized and recorded by a data acquisition system. This report describes the goals, design, and status of the system. (author)

  8. NEA incident reporting system: Three years' experience

    International Nuclear Information System (INIS)

    Otsuka, Y.; Haeussermann, W.

    1984-01-01

    The paper presents an overview of the NEA Incident Reporting System (IRS) which was set up to collect, assess and disseminate on safety-related incidents in nuclear power plants. The IRS information exchange is significant in two senses. First, it enables regulatory authorities and utilities in participating countries to take appropriate action to prevent the reported mishaps occurring again elsewhere. Secondly, the continuous collection and systematic analysis of such information allows identification of areas of concern where safety research should be strengthened. There are two stages in the IRS information exchange. First, the national IRS Co-ordinator selects information on significant incidents, in accordance with a common reporting threshold, from the abnormal occurrences reported to the regulatory body, to be distributed through the NEA Secretariat. This screening is intended to exclude minor events, so that only significant information is sent to participating countries. Secondly, a group of experts periodically reviews the incidents reported during the preceding twelve months to identify major areas of concern. To assist this process, a computer-based data retrieval system is being developed for IRS incident reports. The paper gives some details of the IRS mechanism and discusses reporting criteria and the information included in a report. Areas of concern derived from reported incidents, an outline of the data retrieval system, and examples of feedback of lessons learned and possibilities for international co-operation are also discussed. (author)

  9. Aviation Safety Reporting System: Process and Procedures

    Science.gov (United States)

    Connell, Linda J.

    1997-01-01

    The Aviation Safety Reporting System (ASRS) was established in 1976 under an agreement between the Federal Aviation Administration (FAA) and the National Aeronautics and Space Administration (NASA). This cooperative safety program invites pilots, air traffic controllers, flight attendants, maintenance personnel, and others to voluntarily report to NASA any aviation incident or safety hazard. The FAA provides most of the program funding. NASA administers the program, sets its policies in consultation with the FAA and aviation community, and receives the reports submitted to the program. The FAA offers those who use the ASRS program two important reporting guarantees: confidentiality and limited immunity. Reports sent to ASRS are held in strict confidence. More than 350,000 reports have been submitted since the program's beginning without a single reporter's identity being revealed. ASRS removes all personal names and other potentially identifying information before entering reports into its database. This system is a very successful, proof-of-concept for gathering safety data in order to provide timely information about safety issues. The ASRS information is crucial to aviation safety efforts both nationally and internationally. It can be utilized as the first step in safety by providing the direction and content to informed policies, procedures, and research, especially human factors. The ASRS process and procedures will be presented as one model of safety reporting feedback systems.

  10. Efficacy of sorafenib, a multi-tyrosine kinase inhibitor, in an adenoid cystic carcinoma metastatic to the lung: case report and review of literature

    Directory of Open Access Journals (Sweden)

    Santos Edgardo S

    2011-09-01

    Full Text Available Abstract Introduction Treatment for squamous cell carcinoma of the head and neck has significantly improved with the addition of cetuximab, a monoclonal antibody against the epidermal growth factor receptor, to conventional cytotoxic agents. The most significant aspect of this treatment approach is the proof that head and neck cancers are suitable for targeted therapies as has been shown in other malignancies. Unfortunately, there are other rare histologic types of head and neck cancer such as adenocarcinoma and adenoid cystic carcinoma. The latter has traditionally been considered to be chemotherapy resistant and surgical resection with or without adjuvant radiation therapy has been the rule as far as treatment is concerned. The course of adenoid cystic carcinoma ranges from indolent to aggressive; however, most patients succumb to the disease as a result of distant metastases. This clinical scenario poses a challenge to oncologists. Several conventional chemotherapy regimens and novel targeted agents have been tried in this rare histologic subtype without success. Case presentation In this case report, we present a 59-year-old Caucasian female with refractory adenoid cystic carcinoma of the maxilla metastatic to the lung that responded to sorafenib, a novel multi-tyrosine kinase inhibitor, which targets angiogenesis, Raf kinase pathway, platelet-derived growth factor Ret, and c-Kit. Conclusion This case illustrates the possibility that this chemoresistant tumor may need the inhibition or blocking of several oncogenic pathways. Certainly, it is imperative that more studies are done in this special population trying to identify tumorigenesis mechanisms that may be upregulated in this malignancy and could be potential targets for therapeutic development.

  11. Structure of a NAD kinase from Thermotoga maritima at 2.3 Å resolution

    International Nuclear Information System (INIS)

    Oganesyan, Vaheh; Huang, Candice; Adams, Paul D.; Jancarik, Jaru; Yokota, Hisao A.; Kim, Rosalind; Kim, Sung-Hou

    2005-01-01

    The expression, purification, crystallization, and structure determination of NAD-kinase from T. maritima are reported. Similarity to other NAD-kinases as well as homo-oligomrization state of the enzyme from T. maritima are discussed. NAD kinase is the only known enzyme that catalyzes the formation of NADP, a coenzyme involved in most anabolic reactions and in the antioxidant defense system. Despite its importance, very little is known regarding the mechanism of catalysis and only recently have several NAD kinase structures been deposited in the PDB. Here, an independent investigation of the crystal structure of inorganic polyphosphate/ATP-NAD kinase, PPNK-THEMA, a protein from Thermotoga maritima, is reported at a resolution of 2.3 Å. The crystal structure was solved using single-wavelength anomalous diffraction (SAD) data collected at the Se absorption-peak wavelength in a state in which no cofactors or substrates were bound. It revealed that the 258-amino-acid protein is folded into two distinct domains, similar to recently reported NAD kinases. The N-terminal α/β-domain spans the first 100 amino acids and the last 30 amino acids of the polypeptide and has several topological matches in the PDB, whereas the other domain, which spans the middle 130 residues, adopts a unique β-sandwich architecture and only appreciably matches the recently deposited PDB structures of NAD kinases

  12. Molecular Imaging with Activatable Reporter Systems

    Directory of Open Access Journals (Sweden)

    Gang Niu, Xiaoyuan Chen

    2012-01-01

    Full Text Available Molecular imaging is a newly emerged multiple disciplinary field that aims to visualize, characterize and quantitatively measure biological processes at cellular and molecular levels in humans and other living systems. A reporter gene is a piece of DNA encoding reporter protein, which presents as a readily measurable phenotype that can be distinguished easily from the background of endogenous protein. After being transferred into cells of organ systems (transgenes, the reporter gene can be utilized to visualize transcriptional and posttranscriptional regulation of gene expression, protein-protein interactions, or trafficking of proteins or cells in living subjects. Herein, we review previous classification of reporter genes and regroup the reporter gene based imaging as basic, inducible and activatable, based on the regulation of reporter gene transcription and post-translational modification of reporter proteins. We then focus on activatable reporters, in which the signal can be activated at the posttranslational level for visualizing protein-protein interactions, protein phosphorylation or tertiary structure changes. The applications of several types of activatable reporters will also be summarized. We conclude that activatable reporter imaging can benefit both basic biomedical research and drug development.

  13. Consultation system for image diagnosis: Report formation support system

    International Nuclear Information System (INIS)

    Ikeda, M.; Sakuma, S.; Ishigaki, T.; Suzuki, K.; Oikawa, K.

    1987-01-01

    The authors developed a consultation system for image diagnosis, involving artificial intelligence ideas. In this system, the authors proposed a new report formation support system and implemented it in lymphangiography. This support system starts with the input of image interpretation. The input process is made mainly by selecting items. This system encodes the input findings into the semantic network, which is represented as a directed graph, and it reserves them into the knowledge database in the above structure. Finally, the output (report) is made in the near natural language, which corresponds to the input findings

  14. Systems Analysis Department annual progress report 1999

    DEFF Research Database (Denmark)

    2000-01-01

    This report describes the work of the Systems Analysis Department at Risø National Laboratory during 1999. The department is undertaking research within Energy Systems Analysis, Energy, Environment and Development Planning - UNEP Centre, Safety,Realiability and Human Factors, and Technology...

  15. Affirmative Action Data Collection and Reporting System.

    Science.gov (United States)

    McLean, Marquita; And Others

    A computerized Affirmative Action Data Collection and Reporting System was implemented at the University of Cincinnati to assist in monitoring the progress of the University's Affirmative Action program. The benefits derived from the system were definitely a contributing factor in the University's successful attempt to have its Affirmative Action…

  16. 1998 FFTF annual system assessment reports

    International Nuclear Information System (INIS)

    Guttenberg, S.

    1998-01-01

    The health of FFTF systems was assessed assuming a continued facility standby condition. The review was accomplished in accordance with the guidelines of FFTF-EI-083, Plant Evaluation Program. The attached document includes an executive summary of the significant conclusions and assessment reports for each system evaluated

  17. 1998 FFTF annual system assessment reports

    Energy Technology Data Exchange (ETDEWEB)

    Guttenberg, S.

    1998-03-19

    The health of FFTF systems was assessed assuming a continued facility standby condition. The review was accomplished in accordance with the guidelines of FFTF-EI-083, Plant Evaluation Program. The attached document includes an executive summary of the significant conclusions and assessment reports for each system evaluated.

  18. NASA Aviation Safety Reporting System (ASRS)

    Science.gov (United States)

    Connell, Linda J.

    2017-01-01

    The NASA Aviation Safety Reporting System (ASRS) collects, analyzes, and distributes de-identified safety information provided through confidentially submitted reports from frontline aviation personnel. Since its inception in 1976, the ASRS has collected over 1.4 million reports and has never breached the identity of the people sharing their information about events or safety issues. From this volume of data, the ASRS has released over 6,000 aviation safety alerts concerning potential hazards and safety concerns. The ASRS processes these reports, evaluates the information, and provides selected de-identified report information through the online ASRS Database at http:asrs.arc.nasa.gov. The NASA ASRS is also a founding member of the International Confidential Aviation Safety Systems (ICASS) group which is a collection of other national aviation reporting systems throughout the world. The ASRS model has also been replicated for application to improving safety in railroad, medical, fire fighting, and other domains. This presentation will discuss confidential, voluntary, and non-punitive reporting systems and their advantages in providing information for safety improvements.

  19. Expression of the Grb2-related protein of the lymphoid system in B cell subsets enhances B cell antigen receptor signaling through mitogen-activated protein kinase pathways.

    Science.gov (United States)

    Yankee, Thomas M; Solow, Sasha A; Draves, Kevin D; Clark, Edward A

    2003-01-01

    Adapter proteins play a critical role in regulating signals triggered by Ag receptor cross-linking. These small molecules link receptor proximal events with downstream signaling pathways. In this study, we explore the expression and function of the Grb2-related protein of the lymphoid system (GrpL)/Grb2-related adaptor downstream of Shc adapter protein in human B cells. GrpL is expressed in naive B cells and is down-regulated following B cell Ag receptor ligation. By contrast, germinal center and memory B cells express little or no GrpL. Using human B cell lines, we detected constitutive interactions between GrpL and B cell linker protein, Src homology (SH)2 domain-containing leukocyte protein of 76 kDa, hemopoietic progenitor kinase 1, and c-Cbl. The N-terminal SH3 domain of GrpL binds c-Cbl while the C-terminal SH3 domain binds B cell linker protein and SH2 domain-containing leukocyte protein of 76 kDa. Exogenous expression of GrpL in a GrpL-negative B cell line leads to enhanced Ag receptor-induced extracellular signal-related kinase and p38 mitogen-activated protein kinase phosphorylation. Thus, GrpL expression in human B cell subsets appears to regulate Ag receptor-mediated signaling events.

  20. Operating experience feedback report - Air systems problems

    International Nuclear Information System (INIS)

    Ornstein, H.L.

    1987-12-01

    This report highlights significant operating events involving observed or potential failures of safety-related systems in U.S. plants that resulted from degraded or malfunctioning non-safety grade air systems. Based upon the evaluation of these events, the Office for Analysis and Evaluation of Operational Data (AEOD) concludes that the issue of air systems problems is an important one which requires additional NRC and industry attention. This report also provides AEOD's recommendations for corrective actions to deal with the issue. (author)

  1. Problem reporting management system performance simulation

    Science.gov (United States)

    Vannatta, David S.

    1993-01-01

    This paper proposes the Problem Reporting Management System (PRMS) model as an effective discrete simulation tool that determines the risks involved during the development phase of a Trouble Tracking Reporting Data Base replacement system. The model considers the type of equipment and networks which will be used in the replacement system as well as varying user loads, size of the database, and expected operational availability. The paper discusses the dynamics, stability, and application of the PRMS and addresses suggested concepts to enhance the service performance and enrich them.

  2. Regulation of natural competence by the orphan two-component system sensor kinase ChiS involves a non-canonical transmembrane regulator in Vibrio cholerae.

    Science.gov (United States)

    Yamamoto, Shouji; Mitobe, Jiro; Ishikawa, Takahiko; Wai, Sun Nyunt; Ohnishi, Makoto; Watanabe, Haruo; Izumiya, Hidemasa

    2014-01-01

    In Vibrio cholerae, 41 chitin-inducible genes, including the genes involved in natural competence for DNA uptake, are governed by the orphan two-component system (TCS) sensor kinase ChiS. However, the mechanism by which ChiS controls the expression of these genes is currently unknown. Here, we report the involvement of a novel transcription factor termed 'TfoS' in this process. TfoS is a transmembrane protein that contains a large periplasmic domain and a cytoplasmic AraC-type DNA-binding domain, but lacks TCS signature domains. Inactivation of tfoS abolished natural competence as well as transcription of the tfoR gene encoding a chitin-induced small RNA essential for competence gene expression. A TfoS fragment containing the DNA-binding domain specifically bound to and activated transcription from the tfoR promoter. Intracellular TfoS levels were unaffected by disruption of chiS and coexpression of TfoS and ChiS in Escherichia coli recovered transcription of the chromosomally integrated tfoR::lacZ gene, suggesting that TfoS is post-translationally modulated by ChiS during transcriptional activation; however, this regulation persisted when the canonical phosphorelay residues of ChiS were mutated. The results presented here suggest that ChiS operates a chitin-induced non-canonical signal transduction cascade through TfoS, leading to transcriptional activation of tfoR. © 2013 John Wiley & Sons Ltd.

  3. Preoperational test report, recirculation ventilation systems

    International Nuclear Information System (INIS)

    Clifton, F.T.

    1997-01-01

    This represents a preoperational test report for Recirculation Ventilation Systems, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system provides vapor space cooling of tanks AY1O1, AY102, AZ1O1, AZ102 and supports the ability to exhaust air from each tank. Each system consists of a valved piping loop, a fan, condenser, and moisture separator; equipment is located inside each respective tank farm in its own hardened building. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System

  4. Preoperational test report, recirculation ventilation systems

    Energy Technology Data Exchange (ETDEWEB)

    Clifton, F.T.

    1997-11-11

    This represents a preoperational test report for Recirculation Ventilation Systems, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system provides vapor space cooling of tanks AY1O1, AY102, AZ1O1, AZ102 and supports the ability to exhaust air from each tank. Each system consists of a valved piping loop, a fan, condenser, and moisture separator; equipment is located inside each respective tank farm in its own hardened building. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System.

  5. Electronic Resources Management System: Recommendation Report 2017

    KAUST Repository

    Ramli, Rindra M.

    2017-05-01

    This recommendation report provides an overview of the selection process for the new Electronic Resources Management System. The library has decided to move away from Innovative Interfaces Millennium ERM module. The library reviewed 3 system as potential replacements namely: Proquest 360 Resource Manager, Ex Libris Alma and Open Source CORAL ERMS. After comparing and trialling the systems, it was decided to go for Proquest 360 Resource Manager.

  6. Apollo experience report: Earth landing system

    Science.gov (United States)

    West, R. B.

    1973-01-01

    A brief discussion of the development of the Apollo earth landing system and a functional description of the system are presented in this report. The more significant problems that were encountered during the program, the solutions, and, in general, the knowledge that was gained are discussed in detail. Two appendixes presenting a detailed description of the various system components and a summary of the development and the qualification test programs are included.

  7. Changing Social and Environmental Reporting Systems

    DEFF Research Database (Denmark)

    Kaspersen, Mia; Riise Johansen, Thomas

    2016-01-01

    Based on a case study of a large multinational group, this paper addresses the way in which social and environmental reporting (SER) systems were changed and the consequences and controversies associated with this change. Drawing on Power's work on the processes by which things are made auditable...... via underlying systems, we focus on how and why a specific programme with auditability as its ultimate aim changed the basis on which the external social and environmental report was prepared. Our analysis demonstrates that the perceived alignment with the financial report preparation and the explicit...... pursuit of auditability legitimized SER and paved the way for data systems to be changed. The programme borrowed authority from financial accounting technologies not only to make a system change but also to push SER internally, as we suggest that an intraorganizational group used the programme to ensure...

  8. A customizable, scalable scheduling and reporting system.

    Science.gov (United States)

    Wood, Jody L; Whitman, Beverly J; Mackley, Lisa A; Armstrong, Robert; Shotto, Robert T

    2014-06-01

    Scheduling is essential for running a facility smoothly and for summarizing activities in use reports. The Penn State Hershey Clinical Simulation Center has developed a scheduling interface that uses off-the-shelf components, with customizations that adapt to each institution's data collection and reporting needs. The system is designed using programs within the Microsoft Office 2010 suite. Outlook provides the scheduling component, while the reporting is performed using Access or Excel. An account with a calendar is created for the main schedule, with separate resource accounts created for each room within the center. The Outlook appointment form's 2 default tabs are used, in addition to a customized third tab. The data are then copied from the calendar into either a database table or a spreadsheet, where the reports are generated.Incorporating this system into an institution-wide structure allows integration of personnel lists and potentially enables all users to check the schedule from their desktop. Outlook also has a Web-based application for viewing the basic schedule from outside the institution, although customized data cannot be accessed. The scheduling and reporting functions have been used for a year at the Penn State Hershey Clinical Simulation Center. The schedule has increased workflow efficiency, improved the quality of recorded information, and provided more accurate reporting. The Penn State Hershey Clinical Simulation Center's scheduling and reporting system can be adapted easily to most simulation centers and can expand and change to meet future growth with little or no expense to the center.

  9. The Kinetics of Myosin Light Chain Kinase Activation of Smooth Muscle Myosin in an In Vitro Model System

    OpenAIRE

    Hong, Feng; Facemyer, Kevin C.; Carter, Michael S.; Jackson, Del R.; Haldeman, Brian D.; Ruana, Nick; Sutherland, Cindy; Walsh, Michael P.; Cremo, Christine R.; Baker, Josh E.

    2013-01-01

    During activation of smooth muscle contraction, one myosin light chain kinase (MLCK) molecule rapidly phosphorylates many smooth muscle myosin (SMM) molecules, suggesting that muscle activation rates are influenced by the kinetics of MLCK-SMM interactions. To determine the rate-limiting step underlying activation of SMM by MLCK, we measured the kinetics of calcium-calmodulin (Ca2+-CaM)-MLCK-mediated SMM phosphorylation and the corresponding initiation of SMM-based F-actin motility in an in vi...

  10. Imatinib-induced fulminant liver failure in chronic myeloid leukemia: role of liver transplant and second-generation tyrosine kinase inhibitors: a case report.

    Science.gov (United States)

    Nacif, Lucas Souto; Waisberg, Daniel R; Pinheiro, Rafael Soares; Lima, Fabiana Roberto; Rocha-Santos, Vinicius; Andraus, Wellington; D'Albuquerque, Luiz Carneiro

    2018-03-10

    There is a worldwide problem of acute liver failure and mortality associated with remaining on the waiting for a liver transplant. In this study, we highlight results published in recent years by leading transplant centers in evaluating imatinib-induced acute liver failure in chronic myeloid leukemia and follow-up in liver transplantation. A 36-year-old brown-skinned woman (mixed Brazilian race) diagnosed 1 year earlier with chronic myeloid leukemia was started after delivery of a baby and continued for 6 months with imatinib mesylate (selective inhibitor of Bcr-Abl tyrosine kinase), which induced liver failure. We conducted a literature review using the PubMed database for articles published through September 2017, and we demonstrate a role of liver transplant in this situation for imatinib-induced liver failure. We report previously published results and a successful liver transplant after acute liver failure due to imatinib-induced in chronic myeloid leukemia treatment. We report a case of a successful liver transplant after acute liver failure resulting from imatinib-induced chronic myeloid leukemia treatment. The literature reveals the importance of prompt acute liver failure diagnosis and treatment with liver transplant in selected cases.

  11. Preoperational test report, raw water system

    Energy Technology Data Exchange (ETDEWEB)

    Clifton, F.T.

    1997-10-29

    This represents the preoperational test report for the Raw Water System, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system supplies makeup water to the W-030 recirculation evaporative cooling towers for tanks AY1O1, AY102, AZ1O1, AZ102. The Raw Water pipe riser and associated strainer and valving is located in the W-030 diesel generator building. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System.

  12. Report on analysis of HERBE system

    International Nuclear Information System (INIS)

    1989-07-01

    The objective of this report is the choice of HERBE system configuration and detailed analysis of neutronic characteristics of the chosen configuration. The system is planned to be built at the RB reactor. Neutronic parameters were calculated by computer code VESNA based on transmission probability method using 44 group nuclear data for 28 nuclides. In the first phase, it has been proposed to achieve HERBE system by using fuel elements existing at the RB reactor. It is suggested to build new hybrid system in the RB reactor using new fuel elements that would be produced

  13. Preoperational test report, raw water system

    International Nuclear Information System (INIS)

    Clifton, F.T.

    1997-01-01

    This represents the preoperational test report for the Raw Water System, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system supplies makeup water to the W-030 recirculation evaporative cooling towers for tanks AY1O1, AY102, AZ1O1, AZ102. The Raw Water pipe riser and associated strainer and valving is located in the W-030 diesel generator building. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System

  14. Measuring Kinase Activity-A Global Challenge.

    Science.gov (United States)

    Cann, Marissa L; McDonald, Ian M; East, Michael P; Johnson, Gary L; Graves, Lee M

    2017-11-01

    The kinase enzymes within a cell, known collectively as the kinome, play crucial roles in many signaling pathways, including survival, motility, differentiation, stress response, and many more. Aberrant signaling through kinase pathways is often linked to cancer, among other diseases. A major area of scientific research involves understanding the relationships between kinases, their targets, and how the kinome adapts to perturbations of the cellular system. This review will discuss many of the current and developing methods for studying kinase activity, and evaluate their applications, advantages, and disadvantages. J. Cell. Biochem. 118: 3595-3606, 2017. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

  15. In Vitro and In Vivo Characterization of a Dual-Function Green Fluorescent Protein–HSV1-Thymidine Kinase Reporter Gene Driven by the Human Elongation Factor 1α Promoter

    Directory of Open Access Journals (Sweden)

    Gary D. Luker

    2002-04-01

    Full Text Available Toward the goal of monitoring activity of native mammalian promoters with molecular imaging techniques, we stably transfected DU145 prostate carcinoma cells with a fusion construct of enhanced green fluorescent protein (EGFP and wild-type herpes simplex virus-1 thymidine kinase (HSV1-TK as a reporter gene driven by the promoter for human elongation factor 1α (EF-1α-EGFP-TK. Using this model system, expression of EGFP was quantified by flow cytometry and fluorescence microscopy, while the HSV1-TK component of the reporter was quantified with 8-[3H]ganciclovir (8-[3H]GCV. As analyzed by flow cytometry, passage of EGFP-TK-DU145 transfected cells (ETK in vitro resulted in populations of cells with high and low expression of EGFP over time. High and low ETK cells retained 23-fold and 5-fold more GCV, respectively, than control. While differences in uptake and retention of GCV corresponded to relative expression of the reporter gene in each subpopulation of cells as determined by both flow cytometry (EGFP and quantitative RT-PCR, the correlation was not linear. Furthermore, in high ETK cells, net retention of various radiolabeled nucleoside analogues varied; the rank order was 8-[3H]GCV < 9-(4-fluoro-3-hydroxymethylbutylguanine ([18F]FHBG ≈ 8-[3H]penciclovir (8-[3H]PCV < 2′-fluoro-2′-deoxy-5-iodouracil-beta-d-arabinofuranoside (2-[14C]FIAU. Xenograft tumors of ETK cells in vivo accumulated 2.5-fold more 8-[3H]GCV per gram of tissue and showed greater fluorescence from EGFP than control DU145 cells, demonstrating that the reporter gene functioned in vivo. These data extend previous reports by showing that a human promoter can be detected in vitro and in vivo with a dual-function reporter exploiting optical and radiotracer techniques.

  16. [Patient reported outcome of tyrosine kinase inhibitor related side effects and their impact on daily life in Chinese patients with chronic myeloid leukemia in the chronic phase].

    Science.gov (United States)

    Yu, L; Wang, H B; Jiang, Q

    2016-11-14

    Objective: To explore the impact of patient reported outcome of tyrosine kinase inhibitor (TKI) related side effects on daily life in Chinese patients with chronic myeloid leukemia (CML) in the chronic phase (CP). Methods: From May to November in 2014, anonymous questionnaires were distributed to adult CML patients who were receiving TKI treatment in China. The impact of TKI-related side effects on daily life were assessed by the score of 1 (no impact) to 5 (high impact) from patient self-report. Results: Data from 731 respondents in the CP who reported the score of the impact of TKI-related side effects on daily life were collected. 407 (56%) were male. The median age was 41 years (range, 18 to 88 years). 560 (77%) started TKI treatment within 1 year after diagnosis. With a median treatment duration of 3 years (range, effects were edema ( n =323, 44% ), fatigue ( n =277, 38% ), gastrointestinal disorders ( n =235, 32% ), skin color changes ( n =142, 19% ), muscle cramps ( n =137, 19% ), rash ( n =105, 14% ), hepatic function abnormalities ( n =91, 12%), weight gain ( n =86, 12%), and cytopenia ( n =59, 8%). Multivariate analyses showed that TKI treatment duration effects on daily life in 218 (30%)respondents (1 score). 375 (51%)respondents reported their daily life were slightly or moderately decreased (2 or 3 score), while 138 (19%) significantly decreased (4 or 5 score). Multivariate analyses showed that female, ≥40 years old, use of generic TKI, TKI treatment duration negative effect on their daily life. When taking TKI related side-effects into considderation, secondary school and below, use of generic TKI, TKI treatment duration negative effect on their daily life. Conclusion: Edema, fatigue, gastrointestinal disorders, rash, skin color changes and hepatic function abnormalities were common TKI-related side effects and influenced CML patients' daily life in China. In addition, female, older age, lower education level, use of generic TKI and shorter TKI

  17. Fatty acid-amino acid conjugates are essential for systemic activation of salicylic acid-induced protein kinase and accumulation of jasmonic acid in Nicotiana attenuata.

    Science.gov (United States)

    Hettenhausen, Christian; Heinrich, Maria; Baldwin, Ian T; Wu, Jianqiang

    2014-11-28

    Herbivory induces the activation of mitogen-activated protein kinases (MAPKs), the accumulation of jasmonates and defensive metabolites in damaged leaves and in distal undamaged leaves. Previous studies mainly focused on individual responses and a limited number of systemic leaves, and more research is needed for a better understanding of how different plant parts respond to herbivory. In the wild tobacco Nicotiana attenuata, FACs (fatty acid-amino acid conjugates) in Manduca sexta oral secretions (OS) are the major elicitors that induce herbivory-specific signaling but their role in systemic signaling is largely unknown. Here, we show that simulated herbivory (adding M. sexta OS to fresh wounds) dramatically increased SIPK (salicylic acid-induced protein kinase) activity and jasmonic acid (JA) levels in damaged leaves and in certain (but not all) undamaged systemic leaves, whereas wounding alone had no detectable systemic effects; importantly, FACs and wounding are both required for activating these systemic responses. In contrast to the activation of SIPK and elevation of JA in specific systemic leaves, increases in the activity of an important anti-herbivore defense, trypsin proteinase inhibitor (TPI), were observed in all systemic leaves after simulated herbivory, suggesting that systemic TPI induction does not require SIPK activation and JA increases. Leaf ablation experiments demonstrated that within 10 minutes after simulated herbivory, a signal (or signals) was produced and transported out of the treated leaves, and subsequently activated systemic responses. Our results reveal that N. attenuata specifically recognizes herbivore-derived FACs in damaged leaves and rapidly send out a long-distance signal to phylotactically connected leaves to activate MAPK and JA signaling, and we propose that FACs that penetrated into wounds rapidly induce the production of another long-distance signal(s) which travels to all systemic leaves and activates TPI defense.

  18. Analysis of Hybrid Hydrogen Systems: Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Dean, J.; Braun, R.; Munoz, D.; Penev, M.; Kinchin, C.

    2010-01-01

    Report on biomass pathways for hydrogen production and how they can be hybridized to support renewable electricity generation. Two hybrid systems were studied in detail for process feasibility and economic performance. The best-performing system was estimated to produce hydrogen at costs ($1.67/kg) within Department of Energy targets ($2.10/kg) for central biomass-derived hydrogen production while also providing value-added energy services to the electric grid.

  19. Thymidine kinases in archaea

    DEFF Research Database (Denmark)

    Clausen, A.R.; Matakos, A.; Sandrini, Michael

    2006-01-01

    Twenty-six fully sequenced archaeal genomes were searched for genes coding for putative deoxyribonucleoside kinases (dNKs). We identified only 5 human-like thymidine kinase 1 genes (TK1s) and none for non-TK1 kinases. Four TK1s were identified in the Euryarchaea and one was found in the Crenarcha...

  20. Isolated systems with wind power. Main report

    Energy Technology Data Exchange (ETDEWEB)

    Lundsager, P.; Bindner, H.; Clausen, N.E.; Frandsen, S.; Hansen, L.H.; Hansen, J.C.

    2001-06-01

    The overall objective of this research project is to study the development of methods and guidelines rather than 'universal solutions' for the use of wind energy in isolated communities. The main specific objective of the project is to develop and present a more unified and generally applicable approach for assessing the technical and economical feasibility of isolated power supply systems with wind energy. As a part of the project the following tasks were carried out: Review of literature, field measurements in Egypt, development of an inventory of small isolated systems, overview of end-user demands, analysis of findings and development of proposed guidelines. The project is reported in one main report and four topical reports, all of them issued as Risoe reports. This is the Main Report Risoe-R-1256, summing up the activities and findings of the project and outlining an Implementation Strategy for Isolated Systems with Wind Power, applicable for international organisations such as donor agencies and development banks. (au)

  1. Kinase detection with gallium nitride based high electron mobility transistors.

    Science.gov (United States)

    Makowski, Matthew S; Bryan, Isaac; Sitar, Zlatko; Arellano, Consuelo; Xie, Jinqiao; Collazo, Ramon; Ivanisevic, Albena

    2013-07-01

    A label-free kinase detection system was fabricated by the adsorption of gold nanoparticles functionalized with kinase inhibitor onto AlGaN/GaN high electron mobility transistors (HEMTs). The HEMTs were operated near threshold voltage due to the greatest sensitivity in this operational region. The Au NP/HEMT biosensor system electrically detected 1 pM SRC kinase in ionic solutions. These results are pertinent to drug development applications associated with kinase sensing.

  2. Acceptance test report: Backup power system

    International Nuclear Information System (INIS)

    Cole, D.B.

    1996-01-01

    Acceptance Test Report for construction functional testing of Project W-030 Backup Power System. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. Backup power includes a single 125 KW diesel generator, three 10-kva uninterruptible power supply units, and all necessary control

  3. Energy Innovation Systems Indicator Report 2016

    DEFF Research Database (Denmark)

    Borup, Mads; Klitkou, Antje; Iversen, Eric

    This report collates a set of indicators, figures and tables for the energy innovation system in Denmark. Emphasis is on renewable energy and other technologies for moving towards sustainability. The purpose is to provide an overview of indicators available for illuminating dynamics and character...... in “EIS – Strategic research alliance for Energy Innovation Systems and their dynamics – Denmark in global competition”. EIS is funded by the Danish Council for Strategic Research (Innovation Fund Denmark) and by the involved research organisations.......This report collates a set of indicators, figures and tables for the energy innovation system in Denmark. Emphasis is on renewable energy and other technologies for moving towards sustainability. The purpose is to provide an overview of indicators available for illuminating dynamics...... and characteristics of energy innovation systems and to the extent possible offer figures of the developments in the individual indicators. The report is an update of a report published in 2012. Graphs and numbers are updated with the most recent data available. The text is updated where needed in connection...

  4. Railcar waste transfer system hydrostatic test report

    International Nuclear Information System (INIS)

    Ellingson, S.D.

    1997-01-01

    This Acceptance Test Report (ATR) documents for record purposes the field results, acceptance, and approvals of the completed acceptance test per HNF-SD-W417-ATP-001, ''Rail car Waste Transfer System Hydrostatic Test''. The test was completed and approved without any problems or exceptions

  5. The NSTX Trouble Reporting System; TOPICAL

    International Nuclear Information System (INIS)

    S. Sengupta; G. Oliaro

    2002-01-01

    An online Trouble Reporting System (TRS) has been introduced at the National Spherical Torus Experiment (NSTX). The TRS is used by NSTX operators to report problems that affect NSTX operations. The purpose of the TRS is to enhance NSTX reliability and maintainability by identifying components, occurrences, and trends that contribute to machine downtime. All NSTX personnel have access to the TRS. The user interface is via a web browser, such as Netscape or Internet Explorer. This web-based feature permits any X-terminal, PC, or MAC access to the TRS. The TRS is based upon a trouble reporting system developed at the DIII-D Tokamak, at General Atomics Technologies. This paper will provide a detailed description of the TRS software architecture, user interface, MS SQL server interface and operational experiences. In addition, sample data from the TRS database will be summarized and presented

  6. BMFT-UPTF densitometer system test report

    International Nuclear Information System (INIS)

    Menkhaus, D.E.

    1985-11-01

    This report documents acceptance test results performed on the five Upper Plenum Test Facility (UPTF) three-beam densitometer systems and spare parts. The five densitometer systems are used on the UPTF four hot legs and broken cold leg to measure average chordal-beam densities. The primary objectives of the tests performed were: to verify all assemblies fit as designed (mechanical fitup); to ensure radiation levels met the criteria (<2.5 mR/h); to verify that design accuracy requirements were met (performance tests); and to verify proper operation of the densitometer systems (functional checks). 15 figs., 11 tabs

  7. A High Throughput Screening Assay for Anti-Mycobacterial Small Molecules Based on Adenylate Kinase Release as a Reporter of Cell Lysis.

    Directory of Open Access Journals (Sweden)

    Lauren Forbes

    Full Text Available Mycobacterium tuberculosis (Mtb is well-established to be one of the most important bacterial pathogens for which new antimicrobial therapies are needed. Herein, we describe the development of a high throughput screening assay for the identification of molecules that are bactericidal against Mycobacteria. The assay utilizes the release of the intracellular enzyme adenylate kinase into the culture medium as a reporter of mycobacterial cell death. We demonstrate that the assay is selective for mycobactericidal molecules and detects anti-mycobacterial activity at concentrations below the minimum inhibitory concentration of many molecules. Thus, the AK assay is more sensitive than traditional growth assays. We have validated the AK assay in the HTS setting using the Mtb surrogate organism M. smegmatis and libraries of FDA approved drugs as well as a commercially available Diversity set. The screen of the FDA-approved library demonstrated that the AK assay is able to identify the vast majority of drugs with known mycobactericidal activity. Importantly, our screen of the Diversity set revealed that the increased sensitivity of the AK assay increases the ability of M. smegmatis-based screens to detect molecules with relatively poor activity against M. smegmatis but good to excellent activity against Mtb.

  8. The Message Reporting System of the ATLAS DAQ System

    CERN Document Server

    Caprini, M; Kolos, S; 10th ICATPP Conference on Astroparticle, Particle, Space Physics, Detectors and Medical Physics Applications

    2008-01-01

    The Message Reporting System (MRS) in the ATLAS data acquisition system (DAQ) is one package of the Online Software which acts as a glue of various elements of DAQ, High Level Trigger (HLT) and Detector Control System (DCS). The aim of the MRS is to provide a facility which allows all software components in ATLAS to report messages to other components of the distributed DAQ system. The processes requiring a MRS are on one hand applications that report error conditions or information and on the other hand message processors that receive reported messages. A message reporting application can inject one or more messages into the MRS at any time. An application wishing to receive messages can subscribe to a message group according to defined criteria. The application receives messages that fulfill the subscription criteria when they are reported to MRS. The receiver message processing can consist of anything from simply logging the messages in a file/terminal to performing message analysis. The inter-process comm...

  9. Space reactor electric systems: system integration studies, Phase 1 report

    International Nuclear Information System (INIS)

    Anderson, R.V.; Bost, D.; Determan, W.R.; Harty, R.B.; Katz, B.; Keshishian, V.; Lillie, A.F.; Thomson, W.B.

    1983-01-01

    This report presents the results of preliminary space reactor electric system integration studies performed by Rockwell International's Energy Systems Group (ESG). The preliminary studies investigated a broad range of reactor electric system concepts for powers of 25 and 100 KWe. The purpose of the studies was to provide timely system information of suitable accuracy to support ongoing mission planning activities. The preliminary system studies were performed by assembling the five different subsystems that are used in a system: the reactor, the shielding, the primary heat transport, the power conversion-processing, and the heat rejection subsystems. The subsystem data in this report were largely based on Rockwell's recently prepared Subsystem Technology Assessment Report. Nine generic types of reactor subsystems were used in these system studies. Several levels of technology were used for each type of reactor subsystem. Seven generic types of power conversion-processing subsystems were used, and several levels of technology were again used for each type. In addition, various types and levels of technology were used for the shielding, primary heat transport, and heat rejection subsystems. A total of 60 systems were studied

  10. Preoperational test report, recirculation condenser cooling systems

    International Nuclear Information System (INIS)

    Clifton, F.T.

    1997-01-01

    This represents a preoperational test report for Recirculation Condenser Systems, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The four system provide condenser cooling water for vapor space cooling of tanks AY1O1, AY102, AZ1O1, AZ102. Each system consists of a valved piping loop, a pair of redundant recirculation pumps, a closed-loop evaporative cooling tower, and supporting instrumentation; equipment is located outside the farm on concrete slabs. Piping is routed to the each ventilation condenser inside the farm via below-grade concrete trenches. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System

  11. Preoperational test report, recirculation condenser cooling systems

    Energy Technology Data Exchange (ETDEWEB)

    Clifton, F.T.

    1997-11-04

    This represents a preoperational test report for Recirculation Condenser Systems, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The four system provide condenser cooling water for vapor space cooling of tanks AY1O1, AY102, AZ1O1, AZ102. Each system consists of a valved piping loop, a pair of redundant recirculation pumps, a closed-loop evaporative cooling tower, and supporting instrumentation; equipment is located outside the farm on concrete slabs. Piping is routed to the each ventilation condenser inside the farm via below-grade concrete trenches. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System.

  12. Phosphorylation of varicella-zoster virus glycoprotein gpI by mammalian casein kinase II and casein kinase I

    International Nuclear Information System (INIS)

    Grose, C.; Jackson, W.; Traugh, J.A.

    1989-01-01

    Varicella-zoster virus (VZV) glycoprotein gpI is the predominant viral glycoprotein within the plasma membranes of infected cells. This viral glycoprotein is phosphorylated on its polypeptide backbone during biosynthesis. In this report, the authors investigated the protein kinases which participate in the phosphorylation events. Under in vivo conditions, VZV gpI was phosphorylated on its serine and threonine residues by protein kinases present within lysates of either VZV-infected or uninfected cells. Because this activity was diminished by heparin, a known inhibitor of casein kinase II, isolated gpI was incubated with purified casein kinase II and shown to be phosphorylated in an in vitro assay containing [γ- 32 P]ATP. The same glycoprotein was phosphorylated when [ 32 P]GTP was substituted for [ 32 P]ATP in the protein kinase assay. They also tested whether VZV gpI was phosphorylated by two other ubiquitous mammalian protein kinases--casein kinase I and cyclic AMP-dependent kinase--and found that only casein kinase I modified gpI. When the predicted 623-amino-acid sequence of gpI was examined, two phosphorylation sites known to be optimal for casein kinase II were observed. In summary, this study showed that VZV gpI was phosphorylated by each of two mammalian protein kinases (casein kinase I and casein kinase II) and that potential serine-threonine phosphorylation sites for each of these two kinases were present in the viral glycoprotein

  13. Vitrification Facility integrated system performance testing report

    International Nuclear Information System (INIS)

    Elliott, D.

    1997-01-01

    This report provides a summary of component and system performance testing associated with the Vitrification Facility (VF) following construction turnover. The VF at the West Valley Demonstration Project (WVDP) was designed to convert stored radioactive waste into a stable glass form for eventual disposal in a federal repository. Following an initial Functional and Checkout Testing of Systems (FACTS) Program and subsequent conversion of test stand equipment into the final VF, a testing program was executed to demonstrate successful performance of the components, subsystems, and systems that make up the vitrification process. Systems were started up and brought on line as construction was completed, until integrated system operation could be demonstrated to produce borosilicate glass using nonradioactive waste simulant. Integrated system testing and operation culminated with a successful Operational Readiness Review (ORR) and Department of Energy (DOE) approval to initiate vitrification of high-level waste (HLW) on June 19, 1996. Performance and integrated operational test runs conducted during the test program provided a means for critical examination, observation, and evaluation of the vitrification system. Test data taken for each Test Instruction Procedure (TIP) was used to evaluate component performance against system design and acceptance criteria, while test observations were used to correct, modify, or improve system operation. This process was critical in establishing operating conditions for the entire vitrification process

  14. Acquired resistance L747S mutation in an epidermal growth factor receptor-tyrosine kinase inhibitor-naïve patient: A report of three cases.

    Science.gov (United States)

    Yamaguchi, Fumihiro; Fukuchi, Kunihiko; Yamazaki, Yohei; Takayasu, Hiromi; Tazawa, Sakiko; Tateno, Hidetsugu; Kato, Eisuke; Wakabayashi, Aya; Fujimori, Mami; Iwasaki, Takuya; Hayashi, Makoto; Tsuchiya, Yutaka; Yamashita, Jun; Takeda, Norikazu; Kokubu, Fumio

    2014-02-01

    The purpose of the present study was to report cases of epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI)-naïve patients carrying a mutation associated with acquired resistance to the drug. Gene alterations in 77 lung carcinoma patients were analyzed by collecting and studying curette lavage fluid at the time of diagnosis. PCRs were performed to amplify mutation hotspot regions in EGFR genes. The PCR products were direct-sequenced and the mutations confirmed by resequencing using different primers. Case 1 was a 78-year-old Japanese male diagnosed with stage IB lung adenocarcinoma who was found to have two EGFR mutations, G719S and L747S. Case 2 was a 73-year-old Japanese male diagnosed with stage IV squamous cell lung carcinoma and bone metastasis who had the EGFR mutation, L747S. Case 3 was an 82-year-old Japanese male diagnosed with hyponatremia due to inappropriate secretion of antidiuretic hormone and stage IIIB small cell lung carcinoma (SCLC) who had the EGFR mutation, L747S. Thus, the EGFR mutation L747S associated with acquired EGFR-TKI resistance was detected in two non-small cell lung carcinoma (NSCLC) patients and one SCLC patient, none of whom had ever received EGFR-TKI. The patients were current smokers with stages at diagnosis ranging from IB to IV, and their initial tumors contained resistant clones carrying L747S. L747S may be associated with primary resistance. To the best of our knowledge, this study is the first report of an EGFR mutation associated with resistance to EGFR-TKI in SCLC patients. The early detection of EGFR-TKI resistance mutations may be beneficial in making treatment decisions for lung carcinoma patients, including those with SCLC.

  15. Prognostic impact of the pretreatment aspartate transaminase/alanine transaminase ratio in patients treated with first-line systemic tyrosine kinase inhibitor therapy for metastatic renal cell carcinoma.

    Science.gov (United States)

    Kang, Minyong; Yu, Jiwoong; Sung, Hyun Hwan; Jeon, Hwang Gyun; Jeong, Byong Chang; Park, Se Hoon; Jeon, Seong Soo; Lee, Hyun Moo; Choi, Han Yong; Seo, Seong Il

    2018-05-13

    To examine the prognostic role of the pretreatment aspartate transaminase/alanine transaminase or De Ritis ratio in patients with metastatic renal cell carcinoma receiving first-line systemic tyrosine kinase inhibitor therapy. We retrospectively searched the medical records of 579 patients with metastatic renal cell carcinoma who visited Samsung Medical Center, Seoul, Korea, from January 2001 through August 2016. After excluding 210 patients, we analyzed 360 patients who received first-line tyrosine kinase inhibitor therapy. Cancer-specific survival and overall survival were defined as the primary and secondary end-points, respectively. A multivariate Cox proportional hazards regression model was used to identify independent prognosticators of survival outcomes. The overall population was divided into two groups according to the pretreatment De Ritis ratio as an optimal cut-off value of 1.2, which was determined by a time-dependent receiver operating characteristic curve analysis. Patients with a higher pretreatment De Ritis ratio (≥1.2) had worse cancer-specific survival and overall survival outcomes, compared with those with a lower De Ritis ratio (<1.2). Notably, a higher De Ritis ratio (≥1.2) was found to be an independent predictor of both cancer-specific survival (hazard ratio 1.61, 95% confidence interval 1.13-2.30) and overall survival outcomes (hazard ratio 1.69, 95% confidence interval 1.19-2.39), along with male sex, multiple metastasis (≥2), non-clear cell histology, advanced pT stage (≥3), previous metastasectomy and the Memorial Sloan Kettering Cancer Center risk classification. Our findings show that the pretreatment De Ritis ratio can provide valuable information about the survival outcomes of metastatic renal cell carcinoma patients receiving first-line tyrosine kinase inhibitor therapy. © 2018 The Japanese Urological Association.

  16. Stereotactic Radiation Therapy can Safely and Durably Control Sites of Extra-Central Nervous System Oligoprogressive Disease in Anaplastic Lymphoma Kinase-Positive Lung Cancer Patients Receiving Crizotinib

    Energy Technology Data Exchange (ETDEWEB)

    Gan, Gregory N., E-mail: gregory.gan@ucdenver.edu [Department of Radiation Oncology, University of Colorado, Aurora, Colorado (United States); Weickhardt, Andrew J.; Scheier, Benjamin; Doebele, Robert C. [Department of Medical Oncology, University of Colorado, Aurora, Colorado (United States); Gaspar, Laurie E.; Kavanagh, Brian D. [Department of Radiation Oncology, University of Colorado, Aurora, Colorado (United States); Camidge, D. Ross [Department of Medical Oncology, University of Colorado, Aurora, Colorado (United States)

    2014-03-15

    Purpose: To analyze the durability and toxicity of radiotherapeutic local ablative therapy (LAT) applied to extra-central nervous system (eCNS) disease progression in anaplastic lymphoma kinase-positive non-small cell lung cancer (NSCLC) patients. Methods and Materials: Anaplastic lymphoma kinase-positive NSCLC patients receiving crizotinib and manifesting ≤4 discrete sites of eCNS progression were classified as having oligoprogressive disease (OPD). If subsequent progression met OPD criteria, additional courses of LAT were considered. Crizotinib was continued until eCNS progression was beyond OPD criteria or otherwise not suitable for further LAT. Results: Of 38 patients, 33 progressed while taking crizotinib. Of these, 14 had eCNS progression meeting OPD criteria suitable for radiotherapeutic LAT. Patients with eCNS OPD received 1-3 courses of LAT with radiation therapy. The 6- and 12-month actuarial local lesion control rates with radiation therapy were 100% and 86%, respectively. The 12-month local lesion control rate with single-fraction equivalent dose >25 Gy versus ≤25 Gy was 100% versus 60% (P=.01). No acute or late grade >2 radiation therapy-related toxicities were observed. Median overall time taking crizotinib among those treated with LAT versus those who progressed but were not suitable for LAT was 28 versus 10.1 months, respectively. Patients continuing to take crizotinib for >12 months versus ≤12 months had a 2-year overall survival rate of 72% versus 12%, respectively (P<.0001). Conclusions: Local ablative therapy safely and durably eradicated sites of individual lesion progression in anaplastic lymphoma kinase-positive NSCLC patients receiving crizotinib. A dose–response relationship for local lesion control was observed. The suppression of OPD by LAT in patients taking crizotinib allowed an extended duration of exposure to crizotinib, which was associated with longer overall survival.

  17. Energy innovation systems indicator report 2012

    Energy Technology Data Exchange (ETDEWEB)

    Borup, M. [Technical Univ. of Denmark. DTU Management Engineering, Kgs. Lyngby (Denmark); Klitkou, A.; Iversen, E. [Nordic Institute for Studies in Innovation, Research and Education, Oslo (Norway)

    2012-12-15

    Knowledge about the innovation systems with respect to new energy solutions and technologies is of central importance for understanding the dynamics of change in the energy sector and assessment of opportunities for moving towards more climate-friendly and sustainable energy systems and for socio-economic development in the field, creation of new businesses, work places, etc.. This is the topic that in general is addressed in the research activities of the ''EIS - Strategic research alliance for Energy Innovation Systems and their dynamics - Denmark in global competition''. As part of this, the present report gives an overview of the available indicators of energy innovation systems and points out some of the limitations and potentials there currently are in this connection. Focus is on Denmark. Figures for other countries, primarily Nordic or European, are in some cases showed as well, offering a comparative perspective. (Author)

  18. Roles of Apicomplexan protein kinases at each life cycle stage.

    Science.gov (United States)

    Kato, Kentaro; Sugi, Tatsuki; Iwanaga, Tatsuya

    2012-06-01

    Inhibitors of cellular protein kinases have been reported to inhibit the development of Apicomplexan parasites, suggesting that the functions of protozoan protein kinases are critical for their life cycle. However, the specific roles of these protein kinases cannot be determined using only these inhibitors without molecular analysis, including gene disruption. In this report, we describe the functions of Apicomplexan protein kinases in each parasite life stage and the potential of pre-existing protein kinase inhibitors as Apicomplexan drugs against, mainly, Plasmodium and Toxoplasma. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  19. Critical/non-critical system methodology report

    International Nuclear Information System (INIS)

    1989-01-01

    The method used to determine how the waste Isolation Pilot Plant (WIPP) facilities/systems were classified as critical or non-critical to the receipt of CH waste is described within this report. All WIPP critical facilities/systems are listed in the Operational Readiness Review Dictionary. Using the Final Safety Analysis Report (FSAR) as a guide to define the boundaries of the facilities/systems, a direct correlation of the ORR Dictionary to the FSAR can be obtained. The critical facilities/systems are those which are directly related to or have a critical support role in the receipt of CH waste. The facility/systems must meet one of the following requirements to be considered critical: (a) confinement or measure of the release of radioactive materials; (b) continued receipt and/or storage of transuranic waste (TRU) without an interruption greater than one month according to the shipping plan schedule; (c) the environmental and occupational safety of personnel meets the established site programs; and (d) the physical security of the WIPP facilities

  20. Tehachapi solar thermal system first annual report

    Energy Technology Data Exchange (ETDEWEB)

    Rosenthal, A. [Southwest Technology Development Inst., Las Cruces, NM (US)

    1993-05-01

    The staff of the Southwest Technology Development Institute (SWTDI), in conjunction with the staff of Industrial Solar Technology (IST), have analyzed the performance, operation, and maintenance of a large solar process heat system in use at the 5,000 inmate California Correctional Institution (CCI) in Tehachapi, CA. This report summarizes the key design features of the solar plant, its construction and maintenance histories through the end of 1991, and the performance data collected at the plant by a dedicated on-site data acquisition system (DAS).

  1. PUREX exhaust ventilation system installation test report

    International Nuclear Information System (INIS)

    Blackaby, W.B.

    1997-01-01

    This Acceptance Test Report validates the testing performed, the exceptions logged and resolved and certifies this portion of the SAMCONS has met all design and test criteria to perform as an operational system. The proper installation of the PUREX exhaust ventilation system components and wiring was systematically evaluated by performance of this procedure. Proper operation of PUREX exhaust fan inlet, outlet, and vortex damper actuators and limit switches were verified, using special test equipment, to be correct and installed wiring connections were verified by operation of this equipment

  2. A critical incident reporting system in anaesthesia.

    Science.gov (United States)

    Madzimbamuto, F D; Chiware, R

    2001-01-01

    To audit the recently established Critical Incident Reporting System in the Department of Anaesthesia and Critical Care Medicine, University of Zimbabwe Medical School. The system was set up with the purpose of improving the quality of care delivered by the department. Cross sectional study. A critical incident was defined as 'any adverse and reversible event in theatre, during or immediately after surgery that if it persisted without correction would cause harm to the patient'. The anaesthetic or recovery room staff filled a critical incident form anonymously. Data was collected from critical incident reporting forms for analysis. The anaesthetic service in the two teaching hospitals of Harare Central and Parirenyatwa General Hospitals. Between May and October 2000, 62 completed critical incident forms were collected. The nature of the incident and the monitoring used were recorded, the cause was classified as human, equipment or monitoring failure and the outcome for each patient reported. There was no formal system for reminding staff to fill in their critical incident forms. A total of 14,165 operations were performed over the reporting period: 62 critical incident forms were collected, reporting 130 incidents, giving a rate of 0.92% (130/14,165). Of these, 42 patients were emergencies and 20 elective. The incidents were hypotension, hypoxia, bradycardia, ECG changes, aspiration, laryngospasm, high spinal, and cardiac arrest. Monitoring present on patients who had critical incidents was: capnography 57%, oxymetry 90% and ECG 100%. Other monitors are not reported. Human error contributed in 32/62 of patients and equipment failure in 31/62 of patients. Patient outcome showed 15% died, 23% were unplanned admissions to HDU while 62% were discharged to the ward with little or no adverse outcome. Despite some under reporting, the critical incident rate was within the range reported in the literature. Supervision of juniors is not adequate, especially on call. The

  3. Progressive systemic scleroderma (A case report)

    International Nuclear Information System (INIS)

    Han, Jung Suh; Kwon, Chung Sik

    1974-01-01

    This is a report of a rare case of progressive systemic scleroderma involving the skin, lungs, bones, esophagus, duodenum, and colon in a 27 year old Korean female whose chief complaints were generalized edema, knee joint pain, hard and indurated skin lesions on the anterior chest, neck, both upper and lower extremities with loss of pain and touch sensation for one year. A review of literature is submitted

  4. Progressive systemic scleroderma (A case report)

    Energy Technology Data Exchange (ETDEWEB)

    Han, Jung Suh; Kwon, Chung Sik [Chonnam University College of Medicine, Kwangju (Korea, Republic of)

    1974-10-15

    This is a report of a rare case of progressive systemic scleroderma involving the skin, lungs, bones, esophagus, duodenum, and colon in a 27 year old Korean female whose chief complaints were generalized edema, knee joint pain, hard and indurated skin lesions on the anterior chest, neck, both upper and lower extremities with loss of pain and touch sensation for one year. A review of literature is submitted.

  5. Survival significance of epidermal growth factor receptor tyrosine kinase inhibitors and current staging system for survival after recurrence in patients with completely resected lung adenocarcinoma

    Science.gov (United States)

    Saji, Hisashi; Sakai, Hiroki; Kimura, Hiroyuki; Miyazawa, Tomoyuki; Marushima, Hideki; Nakamura, Haruhiko

    2017-01-01

    Objective We previously reported that the staging system and epidermal growth factor receptor (EGFR) mutation status are key factors for treatment strategy and predicting survival. However, the significance of these factors as predictors of overall survival (OS) and postoperative recurrence survival (PRS) has not been sufficiently elucidated. The objective here was to investigate EGFR mutation status and p-stage, which affect PRS and OS in patients with completely resected lung adenocarcinoma, using a different database. Patients and methods We retrospectively reviewed 56 consecutive lung adenocarcinoma patients with disease recurrence in St. Marianna University Hospital between January 2010 and December 2014. Results EGFR mutants (M) were detected in 16/56 patients (29%). The patients with EGFR M had a better OS than those with EGFR wild-type (WT) status (5-year survival: 50.3% vs 43.1, P=0.133). There was no significant difference in the 3-year recurrence-free survival rate between patients with M and WT (6.3% vs 7.7%, P=0.656), and the patients with EGFR M had a significantly better 3-year PRS than those with WT (77.4% vs 51.7%, P=0.033). The 3-year PRS rate for patients with M/pathologic stage (p-stage) I–II (87.5%) was better than that for patients with M/p-stage III (60.0%), WT/p-stage I–II (52.7%), and WT/p-stage III (43.8%). There was a significant difference between patients with M/p-stage I and WT/p-stage I–II or WT/p-stage III (P=0.021 and 0.030, respectively). During the study period, of the 16 patients with mutants, 12 patients (75%) received EGFR-tyrosine kinase inhibitor (TKI) therapy and among the 40 patients with WT, no patient received EGFR-TKI therapy. Multivariate survival analysis showed that patients with EGFR-TKI therapy had a statistically significant association with favorable PRS (hazard ratio 0.271; 95% confidence interval 0.074–1.000; P=0.050). Conclusion EGFR status and p-stage were found to be essential prognostic factors for

  6. A rice kinase-protein interaction map.

    Science.gov (United States)

    Ding, Xiaodong; Richter, Todd; Chen, Mei; Fujii, Hiroaki; Seo, Young Su; Xie, Mingtang; Zheng, Xianwu; Kanrar, Siddhartha; Stevenson, Rebecca A; Dardick, Christopher; Li, Ying; Jiang, Hao; Zhang, Yan; Yu, Fahong; Bartley, Laura E; Chern, Mawsheng; Bart, Rebecca; Chen, Xiuhua; Zhu, Lihuang; Farmerie, William G; Gribskov, Michael; Zhu, Jian-Kang; Fromm, Michael E; Ronald, Pamela C; Song, Wen-Yuan

    2009-03-01

    Plants uniquely contain large numbers of protein kinases, and for the vast majority of the 1,429 kinases predicted in the rice (Oryza sativa) genome, little is known of their functions. Genetic approaches often fail to produce observable phenotypes; thus, new strategies are needed to delineate kinase function. We previously developed a cost-effective high-throughput yeast two-hybrid system. Using this system, we have generated a protein interaction map of 116 representative rice kinases and 254 of their interacting proteins. Overall, the resulting interaction map supports a large number of known or predicted kinase-protein interactions from both plants and animals and reveals many new functional insights. Notably, we found a potential widespread role for E3 ubiquitin ligases in pathogen defense signaling mediated by receptor-like kinases, particularly by the kinases that may have evolved from recently expanded kinase subfamilies in rice. We anticipate that the data provided here will serve as a foundation for targeted functional studies in rice and other plants. The application of yeast two-hybrid and TAPtag analyses for large-scale plant protein interaction studies is also discussed.

  7. Residual energy applications program systems analysis report

    Energy Technology Data Exchange (ETDEWEB)

    Yngve, P.W.

    1980-10-01

    Current DOE plans call for building an Energy Applied Systems Test (EAST) Facility at the Savannah River Plant in close proximity to the 140 to 150/sup 0/F waste heat from one of several operating nuclear reactors. The waste water flow from each reactor, approximately 165,000 gpm, provides a unique opportunity to test the performance and operating characteristics of large-scale waste heat power generation and heat pump system concepts. This report provides a preliminary description of the potential end-use market, parametric data on heat pump and the power generation system technology, a preliminary listing of EAST Facility requirements, and an example of an integrated industrial park utilizing the technology to maximize economic pay back. The parametric heat pump analysis concluded that dual-fluid Rankine cycle heat pumps with capacities as high as 400 x 10/sup 6/ Btu/h, can utilize large sources of low temperature residual heat to provide 300/sup 0/F saturatd steam for an industrial park. The before tax return on investment for this concept is 36.2%. The analysis also concluded that smaller modular heat pumps could fulfill the same objective while sacrificing only a moderate rate of return. The parametric power generation analysis concluded that multi-pressure Rankine cycle systems not only are superior to single pressure systems, but can also be developed for large systems (approx. = 17 MW/sub e/). This same technology is applicable to smaller systems at the sacrifice of higher investment per unit output.

  8. French power system reliability report 2008

    International Nuclear Information System (INIS)

    Tesseron, J.M.

    2009-06-01

    The reliability of the French power system was fully under control in 2008, despite the power outage in the eastern part of the Provence-Alpes-Cote d'Azur region on November 3, which had been dreaded for several years, since it had not been possible to set up a structurally adequate network. Pursuant to a consultation meeting, the reinforcement solution proposed by RTE was approved by the Minister of Energy, boding well for greater reliability in future. Based on the observations presented in this 2008 Report, RTE's Power System Reliability Audit Mission considers that no new recommendations are needed beyond those expressed in previous reliability reports and during reliability audits. The publication of this yearly report is in keeping with RTE's goal to promote the follow-up over time of the evolution of reliability in its various aspects. RTE thus aims to contribute to the development of reliability culture, by encouraging an improved assessment by the different players (both RTE and network users) of the role they play in building reliability, and by advocating the taking into account of reliability and benchmarking in the European organisations of Transmission System Operators. Contents: 1 - Brief overview of the evolution of the internal and external environment; 2 - Operating situations encountered: climatic conditions, supply / demand balance management, operation of interconnections, management of internal congestion, contingencies affecting the transmission facilities; 3 - Evolution of the reliability reference guide: external reference guide: directives, laws, decrees, etc, ETSO, UCTE, ENTSO-E, contracting contributing to reliability, RTE internal reference guide; 4 - Evolution of measures contributing to reliability in the equipment field: intrinsic performances of components (generating sets, protection systems, operation PLC's, instrumentation and control, automatic frequency and voltage controls, transmission facilities, control systems, load

  9. Myosin light chain kinase phosphorylation in tracheal smooth muscle

    International Nuclear Information System (INIS)

    Stull, J.T.; Hsu, L.C.; Tansey, M.G.; Kamm, K.E.

    1990-01-01

    Purified myosin light chain kinase from smooth muscle is phosphorylated by cyclic AMP-dependent protein kinase, protein kinase C, and the multifunctional calmodulin-dependent protein kinase II. Because phosphorylation in a specific site (site A) by any one of these kinases desensitizes myosin light chain kinase to activation by Ca2+/calmodulin, kinase phosphorylation could play an important role in regulating smooth muscle contractility. This possibility was investigated in 32 P-labeled bovine tracheal smooth muscle. Treatment of tissues with carbachol, KCl, isoproterenol, or phorbol 12,13-dibutyrate increased the extent of kinase phosphorylation. Six primary phosphopeptides (A-F) of myosin light chain kinase were identified. Site A was phosphorylated to an appreciable extent only with carbachol or KCl, agents which contract tracheal smooth muscle. The extent of site A phosphorylation correlated to increases in the concentration of Ca2+/calmodulin required for activation. These results show that cyclic AMP-dependent protein kinase and protein kinase C do not affect smooth muscle contractility by phosphorylating site A in myosin light chain kinase. It is proposed that phosphorylation of myosin light chain kinase in site A in contracting tracheal smooth muscle may play a role in the reported desensitization of contractile elements to activation by Ca2+

  10. Preliminary Systems Design Study assessment report

    International Nuclear Information System (INIS)

    Mayberry, J.L.; Feizollahi, F.; Del Signore, J.C.

    1992-01-01

    The System Design Study (SDS), part of the Waste Technology Development Department at the Idaho National Engineering Laboratory (INEL), examined techniques available for the remediation of hazardous and transuranic waste stored at the Radioactive Waste Management Complex's Subsurface Disposal Area at the INEL. Using specific technologies, system concepts for treating the buried waste and the surrounding contaminated soil were evaluated. Evaluation included implementability, effectiveness, and cost. The SDS resulted in the development of technology requirements including demonstration, testing, and evaluation activities needed for implementing each concept. This volume of the Systems Design Study contain four Appendixes that were part of the study. Appendix A is an EG ampersand G Idaho, Inc., report that represents a review and compilation of previous reports describing the wastes and quantities disposed in the Subsurface Disposal Area of the Idaho National Engineering Laboratory. Appendix B contains the process flowsheets considered in this study, but not selected for detailed analysis. Appendix C is a historical tabulation of radioactive waste incinerators. Appendix D lists Department of Energy facilities where cementation stabilization systems have been used

  11. Final Report: Hydrogen Storage System Cost Analysis

    Energy Technology Data Exchange (ETDEWEB)

    James, Brian David [Strategic Analysis Inc., Arlington, VA (United States); Houchins, Cassidy [Strategic Analysis Inc., Arlington, VA (United States); Huya-Kouadio, Jennie Moton [Strategic Analysis Inc., Arlington, VA (United States); DeSantis, Daniel A. [Strategic Analysis Inc., Arlington, VA (United States)

    2016-09-30

    The Fuel Cell Technologies Office (FCTO) has identified hydrogen storage as a key enabling technology for advancing hydrogen and fuel cell power technologies in transportation, stationary, and portable applications. Consequently, FCTO has established targets to chart the progress of developing and demonstrating viable hydrogen storage technologies for transportation and stationary applications. This cost assessment project supports the overall FCTO goals by identifying the current technology system components, performance levels, and manufacturing/assembly techniques most likely to lead to the lowest system storage cost. Furthermore, the project forecasts the cost of these systems at a variety of annual manufacturing rates to allow comparison to the overall 2017 and “Ultimate” DOE cost targets. The cost breakdown of the system components and manufacturing steps can then be used to guide future research and development (R&D) decisions. The project was led by Strategic Analysis Inc. (SA) and aided by Rajesh Ahluwalia and Thanh Hua from Argonne National Laboratory (ANL) and Lin Simpson at the National Renewable Energy Laboratory (NREL). Since SA coordinated the project activities of all three organizations, this report includes a technical description of all project activity. This report represents a summary of contract activities and findings under SA’s five year contract to the US Department of Energy (Award No. DE-EE0005253) and constitutes the “Final Scientific Report” deliverable. Project publications and presentations are listed in the Appendix.

  12. Sequencing Information Management System (SIMS). Final report

    Energy Technology Data Exchange (ETDEWEB)

    Fields, C.

    1996-02-15

    A feasibility study to develop a requirements analysis and functional specification for a data management system for large-scale DNA sequencing laboratories resulted in a functional specification for a Sequencing Information Management System (SIMS). This document reports the results of this feasibility study, and includes a functional specification for a SIMS relational schema. The SIMS is an integrated information management system that supports data acquisition, management, analysis, and distribution for DNA sequencing laboratories. The SIMS provides ad hoc query access to information on the sequencing process and its results, and partially automates the transfer of data between laboratory instruments, analysis programs, technical personnel, and managers. The SIMS user interfaces are designed for use by laboratory technicians, laboratory managers, and scientists. The SIMS is designed to run in a heterogeneous, multiplatform environment in a client/server mode. The SIMS communicates with external computational and data resources via the internet.

  13. Final report on the FMIT Control System

    International Nuclear Information System (INIS)

    Johnson, J.A.

    1985-01-01

    The computer control system for the Fusion Materials Irradiation Test Facility (FMIT) prototype accelerator was designed using distributed intelligence driven by a distributed database. The system consists of two minicomputers in the central control room and four microcomputers residing in CAMAC crates located near appropriate subsystems of the accelerator. The system uses single vendor hardware as much as practical in an attempt to minimize the maintenance problems. Local control consoles are an integral part of each node computer to provide subsystem check-out. The main console is located in the central control room and permits one-point operation of the complete control system. Automatic surveillance is provided for each data channel by the node computer with out-of-bounds alarms sent to the main console. Report by exception is used for data logging. This control system has been operational for two years. The computers are too heavily loaded and the operator response is slower than desired. A system upgrade to a faster local-area network has been undertaken and is scheduled to be operational by conference time

  14. Brief Report: Blockade of TANK-Binding Kinase 1/IKKɛ Inhibits Mutant Stimulator of Interferon Genes (STING)-Mediated Inflammatory Responses in Human Peripheral Blood Mononuclear Cells.

    Science.gov (United States)

    Frémond, Marie-Louise; Uggenti, Carolina; Van Eyck, Lien; Melki, Isabelle; Bondet, Vincent; Kitabayashi, Naoki; Hertel, Christina; Hayday, Adrian; Neven, Bénédicte; Rose, Yoann; Duffy, Darragh; Crow, Yanick J; Rodero, Mathieu P

    2017-07-01

    Gain-of-function mutations in TMEM173, encoding the stimulator of interferon (IFN) genes (STING) protein, underlie a novel type I interferonopathy that is minimally responsive to conventional immunosuppressive therapies and associated with high frequency of childhood morbidity and mortality. STING gain-of-function causes constitutive oversecretion of IFN. This study was undertaken to determine the effects of a TANK-binding kinase 1 (TBK-1)/IKKɛ inhibitor (BX795) on secretion and signaling of IFN in primary peripheral blood mononuclear cells (PBMCs) from patients with mutations in STING. PBMCs from 4 patients with STING-associated disease were treated with BX795. The effect of BX795 on IFN pathways was assessed by Western blotting and an IFNβ reporter assay, as well as by quantification of IFNα in cell lysates, staining for STAT-1 phosphorylation, and measurement of IFN-stimulated gene (ISG) messenger RNA (mRNA) expression. Treatment of PBMCs with BX795 inhibited the phosphorylation of IFN regulatory factor 3 and IFNβ promoter activity induced in HEK 293T cells by cyclic GMP-AMP or by genetic activation of STING. In vitro exposure to BX795 inhibited IFNα production in PBMCs of patients with STING-associated disease without affecting cell survival. In addition, BX795 decreased STAT-1 phosphorylation and ISG mRNA expression independent of IFNα blockade. These findings demonstrate the effect of BX795 on reducing type I IFN production and IFN signaling in cells from patients with gain-of-function mutations in STING. A combined inhibition of TBK-1 and IKKɛ therefore holds potential for the treatment of patients carrying STING mutations, and may also be relevant in other type I interferonopathies. © 2017, American College of Rheumatology.

  15. Effects of the oral Janus kinase inhibitor tofacitinib on patient-reported outcomes in patients with active rheumatoid arthritis: results of two Phase 2 randomised controlled trials.

    Science.gov (United States)

    Wallenstein, Gene V; Kanik, Keith S; Wilkinson, Bethanie; Cohen, Stanley; Cutolo, Maurizio; Fleischmann, Roy; Genovese, Mark C; Gomez Reino, Juan; Gruben, David; Kremer, Joel; Krishnaswami, Sriram; Lee, Eun Bong; Pascual-Ramos, Virginia; Strand, Vibeke; Zwillich, Samuel H

    2016-01-01

    Tofacitinib is an oral Janus kinase inhibitor for the treatment of rheumatoid arthritis (RA). Here we investigated the effects of tofacitinib on patient-reported outcomes (PRO) in patients with active RA. Two, 6-month, double-blind, placebo-controlled Phase 2b studies were performed. The combination study evaluated patients with inadequate response to methotrexate who received tofacitinib 1-15 mg twice daily (BID), 20 mg once daily or placebo, on background methotrexate. In the monotherapy study, patients with inadequate response to disease-modifying anti-rheumatic drugs received tofacitinib 1-15 mg BID, adalimumab 40 mg once every other week or placebo. PROs measured were: Patient's Assessment of Arthritis Pain (PAAP), Patient's Assessment of Disease Activity, HAQ-DI, FACIT-F and SF-36. In the combination study (n=507), significant improvements (ptofacitinib groups. In the monotherapy study (n=384), significant improvements in PAAP were observed at Week 12 for tofacitinib 5, 10 and 15 mg BID, and in HAQ-DI for tofacitinib 3, 5, 10 and 15 mg BID. Significant improvements versus placebo were seen at Week 2 in PAAP (both studies) and HAQ‑DI (monotherapy study) with tofacitinib, and were maintained throughout each study. In both studies, improvements in several domains of the SF-36 in the tofacitinib groups were observed at Weeks 12 and 24. In patients with active RA, tofacitinib, either in combination with methotrexate or as monotherapy, demonstrated rapid and sustained improvement in pain, physical functioning and health-related quality of life.

  16. Environmental Systems Research FY-99 annual report

    International Nuclear Information System (INIS)

    Miller, D.L.

    2000-01-01

    The Environmental Systems Research (ESR) Program, a part of the Environmental Systems Research and Analysis (ESRA) Program, was implemented to enhance and augment the technical capabilities of the Idaho National Engineering and Environmental Laboratory (INEEL). The purpose for strengthening technical capabilities of the INEEL is to provide the technical base to serve effectively as the Environmental Management Laboratory for the Department of Energy's Office of Environmental Management (EM). The original portfolio of research activities was assembled after an analysis of the EM technology development and science needs as gathered by the Site Technology Coordination Groups (STCGs) complex-wide. Current EM investments in science and technology throughout the research community were also included in this analysis to avoid duplication of efforts. This is a progress report for the second year of the ESR Program (Fiscal Year 99). A report of activities is presented for the five ESR research investment areas: (a) Transport Aspects of Selective Mass Transport Agents, (b) Chemistry of Environmental Surfaces, (c) Materials Dynamics, (d) Characterization Science, and (e) Computational Simulation of Mechanical and Chemical Systems. In addition to the five technical areas, activities in the Science and Technology Foundations element of the program, e.g., interfaces between ESR and the EM Science Program (EMSP) and the EM Focus Areas, are described

  17. Environmental Systems Research, FY-99 Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    Miller, David Lynn

    2000-01-01

    The Environmental Systems Research (ESR) Program, a part of the Environmental Systems Research and Analysis (ESRA) Program, was implemented to enhance and augment the technical capabilities of the Idaho National Engineering and Environmental Laboratory (INEEL). The purpose for strengthening technical capabilities of the INEEL is to provide the technical base to serve effectively as the Environmental Management Laboratory for the Department of Energy's Office of Environmental Management (EM). The original portfolio of research activities was assembled after an analysis of the EM technology development and science needs as gathered by the Site Technology Coordination Groups (STCGs) complex-wide. Current EM investments in science and technology throughout the research community were also included in this analysis to avoid duplication of efforts. This is a progress report for the second year of the ESR Program (Fiscal Year 99). A report of activities is presented for the five ESR research investment areas: (a) Transport Aspects of Selective Mass Transport Agents, (b) Chemistry of Environmental Surfaces, (c) Materials Dynamics, (d) Characterization Science, and (e) Computational Simulation of Mechanical and Chemical Systems. In addition to the five technical areas, activities in the Science and Technology Foundations element of the program, e.g., interfaces between ESR and the EM Science Program (EMSP) and the EM Focus Areas, are described.

  18. Environmental Systems Research FY-99 Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    Miller, D.L.

    2000-01-01

    The Environmental Systems Research (ESR) Program, a part of the Environmental Systems Research and Analysis (ESRA) Program, was implemented to enhance and augment the technical capabilities of the Idaho National Engineering and Environmental Laboratory (INEEL). The purpose for strengthening technical capabilities of the INEEL is to provide the technical base to serve effectively as the Environmental Management Laboratory for the Department of Energy's Office of Environmental Management (EM). The original portfolio of research activities was assembled after an analysis of the EM technology development and science needs as gathered by the Site Technology Coordination Groups (STCGs) complex-wide. Current EM investments in science and technology throughout the research community were also included in this analysis to avoid duplication of efforts. This is a progress report for the second year of the ESR Program (Fiscal Year 99). A report of activities is presented for the five ESR research investment areas: (a) Transport Aspects of Selective Mass Transport Agents, (b) Chemistry of Environmental Surfaces, (c) Materials Dynamics, (d) Characterization Science, and (e) Computational Simulation of Mechanical and Chemical Systems. In addition to the five technical areas, activities in the Science and Technology Foundations element of the program, e.g., interfaces between ESR and the EM Science Program (EMSP) and the EM Focus Areas, are described.

  19. Fluorescence Polarization Screening Assays for Small Molecule Allosteric Modulators of ABL Kinase Function.

    Science.gov (United States)

    Grover, Prerna; Shi, Haibin; Baumgartner, Matthew; Camacho, Carlos J; Smithgall, Thomas E

    2015-01-01

    The ABL protein-tyrosine kinase regulates intracellular signaling pathways controlling diverse cellular processes and contributes to several forms of cancer. The kinase activity of ABL is repressed by intramolecular interactions involving its regulatory Ncap, SH3 and SH2 domains. Small molecules that allosterically regulate ABL kinase activity through its non-catalytic domains may represent selective probes of ABL function. Here we report a screening assay for chemical modulators of ABL kinase activity that target the regulatory interaction of the SH3 domain with the SH2-kinase linker. This fluorescence polarization (FP) assay is based on a purified recombinant ABL protein consisting of the N-cap, SH3 and SH2 domains plus the SH2-kinase linker (N32L protein) and a short fluorescein-labeled probe peptide that binds to the SH3 domain. In assay development experiments, we found that the probe peptide binds to the recombinant ABL N32L protein in vitro, producing a robust FP signal that can be competed with an excess of unlabeled peptide. The FP signal is not observed with control N32L proteins bearing either an inactivating mutation in the SH3 domain or enhanced SH3:linker interaction. A pilot screen of 1200 FDA-approved drugs identified four compounds that specifically reduced the FP signal by at least three standard deviations from the untreated controls. Secondary assays showed that one of these hit compounds, the antithrombotic drug dipyridamole, enhances ABL kinase activity in vitro to a greater extent than the previously described ABL agonist, DPH. Docking studies predicted that this compound binds to a pocket formed at the interface of the SH3 domain and the linker, suggesting that it activates ABL by disrupting this regulatory interaction. These results show that screening assays based on the non-catalytic domains of ABL can identify allosteric small molecule regulators of kinase function, providing a new approach to selective drug discovery for this important

  20. Expression Profiling of Tyrosine Kinase Genes

    National Research Council Canada - National Science Library

    Weier, Heinz

    2000-01-01

    ... of these genes parallels the progression of tumors to a more malignant phenotype. We developed a DNA micro-array based screening system to monitor the level of expression of tyrosine kinase (tk...

  1. SatisFactory Final System Evaluation Report

    OpenAIRE

    Sunlight SA

    2018-01-01

    The present document is a deliverable of the SatisFactory project, funded by the European Commission’s Directorate-General for Research and Innovation (DG RTD), under its Horizon 2020 Research and innovation programme (H2020). The main objective of this deliverable is to report on the SatisFactory Final System Evaluation, with regards to the industrial pilots at COMAU and SUNLIGHT. The evaluation of SatisFactory platform is based on the implementation of the business scenarios where each tool...

  2. Radioactive contamination mapping system detailed design report

    International Nuclear Information System (INIS)

    Bauer, R.G.; O'Callaghan, P.B.

    1996-08-01

    The Hanford Site's 100 Area production reactors released radioactively and chemically contaminated liquids into the soil column. The primary source of the contaminated liquids was reactor coolant and various waste waters released from planned liquid discharges, as well as pipelines, pipe junctions, and retention basins leaking into the disposal sites. Site remediation involves excavating the contaminated soils using conventional earthmoving techniques and equipment, treating as appropriate, transporting the soils, and disposing the soils at ERDF. To support remediation excavation, disposal, and documentation requirements, an automated radiological monitoring system was deemed necessary. The RCMS (Radioactive Contamination Mapping System) was designed to fulfill this need. This Detailed Design Report provides design information for the RCMS in accordance with Bechtel Hanford, Inc. Engineering Design Project Instructions

  3. Power system EMP protection. Final report

    International Nuclear Information System (INIS)

    Marable, J.H.; Barnes, P.R.; Nelson, D.B.

    1975-05-01

    Voltage transients induced in electric power lines and control circuits by the electromagnetic pulse (EMP) from high-altitude nuclear detonations may cause widespread power failure and damage in electric power systems. This report contains a parametric study of EMP power line surges and discusses protective measures to minimize their effects. Since EMP surges have considerably greater rates of rise than lightning surges, recommended standards and test procedures are given to assure that surge arresters protect equipment from damage by EMP. Expected disturbances and damage to power systems are reviewed, and actions are presented which distribution companies can take to counter them. These include backup communications methods, stockpiling of vulnerable parts, repair procedures, and dispatcher actions to prevent blackout from EMP-caused instabilities. A long-range program is presented for improving distributors' protection against EMP. This involves employee training, hardware protection for power and control circuits, and improvement of plans for emergency action. (U.S.)

  4. Reporter Concerns in 300 Mode-Related Incident Reports from NASA's Aviation Safety Reporting System

    Science.gov (United States)

    McGreevy, Michael W.

    1996-01-01

    A model has been developed which represents prominent reporter concerns expressed in the narratives of 300 mode-related incident reports from NASA's Aviation Safety Reporting System (ASRS). The model objectively quantifies the structure of concerns which persist across situations and reporters. These concerns are described and illustrated using verbatim sentences from the original narratives. Report accession numbers are included with each sentence so that concerns can be traced back to the original reports. The results also include an inventory of mode names mentioned in the narratives, and a comparison of individual and joint concerns. The method is based on a proximity-weighted co-occurrence metric and object-oriented complexity reduction.

  5. Oncogenic Receptor Tyrosine Kinases Directly Phosphorylate Focal Adhesion Kinase (FAK) as a Resistance Mechanism to FAK-Kinase Inhibitors.

    Science.gov (United States)

    Marlowe, Timothy A; Lenzo, Felicia L; Figel, Sheila A; Grapes, Abigail T; Cance, William G

    2016-12-01

    Focal adhesion kinase (FAK) is a major drug target in cancer and current inhibitors targeted to the ATP-binding pocket of the kinase domain have entered clinical trials. However, preliminary results have shown limited single-agent efficacy in patients. Despite these unfavorable data, the molecular mechanisms that drive intrinsic and acquired resistance to FAK-kinase inhibitors are largely unknown. We have demonstrated that receptor tyrosine kinases (RTK) can directly bypass FAK-kinase inhibition in cancer cells through phosphorylation of FAK's critical tyrosine 397 (Y397). We also showed that HER2 forms a direct protein-protein interaction with the FAK-FERM-F1 lobe, promoting direct phosphorylation of Y397. In addition, FAK-kinase inhibition induced two forms of compensatory RTK reprogramming: (i) the rapid phosphorylation and activation of RTK signaling pathways in RTK High cells and (ii) the long-term acquisition of RTKs novel to the parental cell line in RTK Low cells. Finally, HER2 +: cancer cells displayed resistance to FAK-kinase inhibition in 3D growth assays using a HER2 isogenic system and HER2 + cancer cell lines. Our data indicate a novel drug resistance mechanism to FAK-kinase inhibitors whereby HER2 and other RTKs can rescue and maintain FAK activation (pY397) even in the presence of FAK-kinase inhibition. These data may have important ramifications for existing clinical trials of FAK inhibitors and suggest that individual tumor stratification by RTK expression would be important to predict patient response to FAK-kinase inhibitors. Mol Cancer Ther; 15(12); 3028-39. ©2016 AACR. ©2016 American Association for Cancer Research.

  6. Integrated radwaste treatment system. Final report

    International Nuclear Information System (INIS)

    Baker, M.N.; Houston, H.M.

    1997-10-01

    In May 1988, the West Valley Demonstration Project (WVDP) began pretreating liquid high-level radioactive waste (HLW). This HLW was produced during spent nuclear fuel reprocessing operations that took place at the Western New York Nuclear Service Center from 1966 to 1972. Original reprocessing operations used plutonium/uranium extraction (PUREX) and thorium extraction (THOREX) processes to recover usable isotopes from spent nuclear fuel. The PUREX process produced a nitric acid-based waste stream, which was neutralized by adding sodium hydroxide to it. About two million liters of alkaline liquid HLW produced from PUREX neutralization were stored in an underground carbon steel tank identified as Tank 8D-2. The THOREX process, which was used to reprocess one core of mixed uranium-thorium fuel, resulted in about 31,000 liters of acidic waste. This acidic HLW was stored in an underground stainless steel tank identified as Tank 8D-4. Pretreatment of the HLW was carried out using the Integrated Radwaste Treatment System (IRTS), from May 1988 until May 1995. This system was designed to decontaminate the liquid HLW, remove salts from it, and encapsulate the resulting waste into a cement waste form that achieved US Nuclear Regulatory Commission (NRC) criteria for low-level waste (LLW) storage and disposal. A thorough discussion of IRTS operations, including all systems, subsystems, and components, is presented in US Department of Energy (DOE) Topical Report (DOE/NE/44139-68), Integrated Radwaste Treatment System Lessons Learned from 2 1/2 Years of Operation. This document also presents a detailed discussion of lessons learned during the first 2 1/2 years of IRTS operation. This report provides a general discussion of all phases of IRTS operation, and presents additional lessons learned during seven years of IRTS operation

  7. Hybrid and rogue kinases encoded in the genomes of model eukaryotes.

    Directory of Open Access Journals (Sweden)

    Ramaswamy Rakshambikai

    Full Text Available The highly modular nature of protein kinases generates diverse functional roles mediated by evolutionary events such as domain recombination, insertion and deletion of domains. Usually domain architecture of a kinase is related to the subfamily to which the kinase catalytic domain belongs. However outlier kinases with unusual domain architectures serve in the expansion of the functional space of the protein kinase family. For example, Src kinases are made-up of SH2 and SH3 domains in addition to the kinase catalytic domain. A kinase which lacks these two domains but retains sequence characteristics within the kinase catalytic domain is an outlier that is likely to have modes of regulation different from classical src kinases. This study defines two types of outlier kinases: hybrids and rogues depending on the nature of domain recombination. Hybrid kinases are those where the catalytic kinase domain belongs to a kinase subfamily but the domain architecture is typical of another kinase subfamily. Rogue kinases are those with kinase catalytic domain characteristic of a kinase subfamily but the domain architecture is typical of neither that subfamily nor any other kinase subfamily. This report provides a consolidated set of such hybrid and rogue kinases gleaned from six eukaryotic genomes-S.cerevisiae, D. melanogaster, C.elegans, M.musculus, T.rubripes and H.sapiens-and discusses their functions. The presence of such kinases necessitates a revisiting of the classification scheme of the protein kinase family using full length sequences apart from classical classification using solely the sequences of kinase catalytic domains. The study of these kinases provides a good insight in engineering signalling pathways for a desired output. Lastly, identification of hybrids and rogues in pathogenic protozoa such as P.falciparum sheds light on possible strategies in host-pathogen interactions.

  8. Design review report for the MCO loading system

    Energy Technology Data Exchange (ETDEWEB)

    Brisbin, S.A.

    1997-06-23

    This design report presents the design of the MCO Loading System. The report includes final design drawings, a system description, failure modes and recovery plans, a system operational description, and stress analysis.

  9. Design review report for the MCO loading system

    International Nuclear Information System (INIS)

    Brisbin, S.A.

    1997-01-01

    This design report presents the design of the MCO Loading System. The report includes final design drawings, a system description, failure modes and recovery plans, a system operational description, and stress analysis

  10. Indolent Systemic Mastocytosis – a Case Report

    Directory of Open Access Journals (Sweden)

    Vujanović Ljuba

    2017-09-01

    Full Text Available Indolent systemic mastocytosis is a benign form of systemic mastocytosis characterized by an abnormal proliferation of mast cells either in the bone marrow or in numerous tissues. Case Report: A 27-year-old female patient was admitted to our department due to urticaria which started a month ago. Before the skin changes appeared, our patient suffered from a toothache, so she took various painkillers (nimesulide, ibuprofen, acetylsalicylic acid, paracetamol. During skin examination, individual hyperpigmented macules on the trunk and lower limbs were observed as incidental findings. The patient reported having them for the last two years. Darier's sign was positive. Following the examination, she was admitted due to suspected urticaria pigmentosa. Laboratory Findings: erythrocyte sedimentation rate: 9 mm/h; complete blood count, urine, blood glucose, total and direct bilirubin, aspartate aminotransferase, alanine aminotransferase, gamma-glutamyl transferase, urea, creatinine, and uric acid were within normal ranges. Electrolytes: sodium, potassium, chlorine clearance, total calcium and calcium ionized, osteocalcin, and crosslaps were within normal ranges as well. Fibrinogen: 5.57 g/l; 5-Hydroxyindoleacetic acid: 49.8 umol/dU (10.4 - 31.2. Bone densitometry, chest x-ray and upper abdomen ultrasound findings were normal. The suspected clinical diagnosis of urticaria pigmentosa was confirmed by skin biopsy. Histopathological examination of the bone marrow showed moderately increased cellularity (60 - 70%. All three types of blood cells were slightly multiplied. Focal infiltrations were found in the perivascular area, consisting of elongated, oval cells with abundant eosinophilic granular cytoplasm. The nuclei were regular, oval shaped with finely granular chromatin and inconspicuous nucleoli. No nuclear atypia was found. These cells are highly CD117-positive. This finding strongly indicated bone marrow infiltration in systemic mastocytosis. The

  11. Metazoan-like signaling in a unicellular receptor tyrosine kinase

    Directory of Open Access Journals (Sweden)

    Schultheiss Kira P

    2013-02-01

    Full Text Available Abstract Background Receptor tyrosine kinases (RTKs are crucial components of signal transduction systems in multicellular animals. Surprisingly, numerous RTKs have been identified in the genomes of unicellular choanoflagellates and other protists. Here, we report the first biochemical study of a unicellular RTK, namely RTKB2 from Monosiga brevicollis. Results We cloned, expressed, and purified the RTKB2 kinase, and showed that it is enzymatically active. The activity of RTKB2 is controlled by autophosphorylation, as in metazoan RTKs. RTKB2 possesses six copies of a unique domain (designated RM2 in its C-terminal tail. An isolated RM2 domain (or a synthetic peptide derived from the RM2 sequence served as a substrate for RTKB2 kinase. When phosphorylated, the RM2 domain bound to the Src homology 2 domain of MbSrc1 from M. brevicollis. NMR structural studies of the RM2 domain indicated that it is disordered in solution. Conclusions Our results are consistent with a model in which RTKB2 activation stimulates receptor autophosphorylation within the RM2 domains. This leads to recruitment of Src-like kinases (and potentially other M. brevicollis proteins and further phosphorylation, which may serve to increase or dampen downstream signals. Thus, crucial features of signal transduction circuitry were established prior to the evolution of metazoans from their unicellular ancestors.

  12. Muscle phosphorylase kinase deficiency

    DEFF Research Database (Denmark)

    Preisler, N; Orngreen, M C; Echaniz-Laguna, A

    2012-01-01

    To examine metabolism during exercise in 2 patients with muscle phosphorylase kinase (PHK) deficiency and to further define the phenotype of this rare glycogen storage disease (GSD).......To examine metabolism during exercise in 2 patients with muscle phosphorylase kinase (PHK) deficiency and to further define the phenotype of this rare glycogen storage disease (GSD)....

  13. Choice of thermal reactor systems: a report

    Energy Technology Data Exchange (ETDEWEB)

    1977-09-01

    This is a report by the UK National Nuclear Corporation published by the UK Secretary of State for Energy (Mr. Benn) on 29th July 1977. It is concerned with the advantages and disadvantages of three thermal reactor systems -the AGR (advanced gas cooled reactor), the PWR (pressurised water reactor), and the SGHWR (steam generating heavy water reactor). The object was to help in the future choice of a thermal system for the UK to cover the next 25 years. The matter of export potential is also considered. A programme of four stations of 1100 to 1300 MW each over six years starting from 1979 was assumed. It is emphasised that a decision must be taken now both about reactor systems and actual orders. Headings are as follows: Extract from conclusions reached; Summary of main features of assessment; General conclusions regarding the following - safety, security of the investment, operational characteristics, development and launching requirements, effect on industry, and capital and generation costs. It is stated that in order to make an overall judgement on reactor choice the technical, commercial and social issues involved must be weighed in conjunction with cost differentials.

  14. The role of brain magnetic resonance imaging in the diagnosis of pantothenate kinase deficiency (previously Hallervorden-Spatz disease): case report; Znaczenie badania tomografii rezonansu magnetycznego mozgowia w diagnostyce deficytu kinazy pantotenowej (dawniej choroby Hallervordena-Spatza) - opis przypadku

    Energy Technology Data Exchange (ETDEWEB)

    Gizewska, M; Bieleninik, A; Adamczyk, T; Walczak, M [II Klinika Chorob Dzieci Pomorskiej Akademii Medycznej, Szczecin (Poland); Cyrylowski, L [Zaklad Diagnostyki Obrazowej i Radiologii Interwencyjnej Pomorskiej Akademii Medycznej, Szczecin (Poland); Abramczyk, G [Oddzial Noworodkow, Patologii i Intensywnej Terapii, Samodzielnego Publicznego Specjalistycznego ZOZ ' ' Zdroje ' ' , Szczecin (Poland); Nowacki, P [Klinika Neurologii Pomorskiej Akademii Medycznej, Szczecin (Poland)

    2005-07-01

    Panthotenate kinase deficiency is a rare metabolic disorder from the group of neuroaxonal dystrophy. It is characterized by symptoms of extrapiramidal system impairment, general dystonia, progressive gait disturbances, limbs rigidity, dyskinesias, choreoatetotic movements, dysarthria and progressive dementia. On the brain MRI, T2 - weighted images demonstrate typical, symmetrical '' eye of the tiger '' sign with hyperintensive signals from the central parts of the pallidum, surrounded by low signals in the pallidum and accompanied by hypointensity in substantia nigra resulting from iron deposition. We present a case of 13.5 year old boy with gait disturbances, increase muscles tone, dysartria, aggressive behavior and learning difficulties progressing from the early childhood. In the differential diagnosis a number of inborn errors of metabolism was excluded. Finding on the brain MRI in T2- weighted images typical picture of '' eye of the tiger '' led to a diagnosis of panthotenate kinase deficiency. In subsequent years of observation, despite attempts of baclofen and calcium panthotenate treatment, progression of pyramidal-extrapyramidal syndrome is observed. In children with symptoms of progressive extrapyramidal tract impairment, a sign of '' eye of the tiger '' with hyperintensive signals from the central parts of the pallidum, surrounded by low signals in the remaining part of the pallidum and accompanied by similar lesions in substantia nigra is typical for panthotenate kinase deficiency - a rare neurodegenerative disorder of central nervous system. (author)

  15. Multi-Point Combustion System: Final Report

    Science.gov (United States)

    Goeke, Jerry; Pack, Spencer; Zink, Gregory; Ryon, Jason

    2014-01-01

    A low-NOx emission combustor concept has been developed for NASA's Environmentally Responsible Aircraft (ERA) program to meet N+2 emissions goals for a 70,000 lb thrust engine application. These goals include 75 percent reduction of LTO NOx from CAEP6 standards without increasing CO, UHC, or smoke from that of current state of the art. An additional key factor in this work is to improve lean combustion stability over that of previous work performed on similar technology in the early 2000s. The purpose of this paper is to present the final report for the NASA contract. This work included the design, analysis, and test of a multi-point combustion system. All design work was based on the results of Computational Fluid Dynamics modeling with the end results tested on a medium pressure combustion rig at the UC and a medium pressure combustion rig at GRC. The theories behind the designs, results of analysis, and experimental test data will be discussed in this report. The combustion system consists of five radially staged rows of injectors, where ten small scale injectors are used in place of a single traditional nozzle. Major accomplishments of the current work include the design of a Multipoint Lean Direct Injection (MLDI) array and associated air blast and pilot fuel injectors, which is expected to meet or exceed the goal of a 75 percent reduction in LTO NOx from CAEP6 standards. This design incorporates a reduced number of injectors over previous multipoint designs, simplified and lightweight components, and a very compact combustor section. Additional outcomes of the program are validation that the design of these combustion systems can be aided by the use of Computational Fluid Dynamics to predict and reduce emissions. Furthermore, the staging of fuel through the individually controlled radially staged injector rows successfully demonstrated improved low power operability as well as improvements in emissions over previous multipoint designs. Additional comparison

  16. Mirror confinement systems: Final technical report

    International Nuclear Information System (INIS)

    1988-08-01

    This report contains: (1) A discussion of azimuthal asymmetrics and fluctuations in RFC-XX-M. Both lead to enhanced radial transport in RFC-XX-M, and presumably most other tandem mirror machines as well; A report on four operating modes of RFC-XX-M which were developed and studied as part of the collaboration. These operating modes were the simple tandem mode, the negative (floating) potential mode, the hot electron mode, and the ECH (electron cyclotron heating) mode; A pulsed rf heated discharge cleaning system which was developed for RFC-XX-M. This method of cleaning proved much more effective than normal glow discharge cleaning, and variations of it are currently in use on the GAMMA-10 tandem mirror and the JIPP TII-U tokamak at the Institute for Plasma Physics at Nagoya; Short descriptions of the diagnostics development and improvement done in conjunction with the work on RFC-XX-M; and a compilation of the work performed at the University of Tsukuba on GAMMA-10. Most of the effort on GAMMA-10 involved diagnostics development and improvement. 16 refs., 42 figs., 1 tab

  17. Schedule Sales Query Report Generation System

    Data.gov (United States)

    General Services Administration — Schedule Sales Query presents sales volume figures as reported to GSA by contractors. The reports are generated as quarterly reports for the current year and the...

  18. Pentek metal coating removal system: Baseline report

    International Nuclear Information System (INIS)

    1997-01-01

    The Pentek coating removal technology was tested and is being evaluated at Florida International University (FIU) as a baseline technology. In conjunction with FIU's evaluation of efficiency and cost, this report covers evaluation conducted for safety and health issues. It is a commercially available technology and has been used for various projects at locations throughout the country. The Pentek coating removal system consisted of the ROTO-PEEN Scaler, CORNER-CUTTER reg-sign, and VAC-PAC reg-sign. They are designed to remove coatings from steel, concrete, brick, and wood. The Scaler uses 3M Roto Peen tungsten carbide cutters while the CORNER-CUTTER reg-sign uses solid needles for descaling activities. These hand tools are used with the VAC-PAC reg-sign vacuum system to capture dust and debris as removal of the coating takes place. The safety and health evaluation during the testing demonstration focused on two main areas of exposure: dust and noise. Dust exposure minimal, but noise exposure was significant. Further testing for each exposure is recommended because of the environment where the testing demonstration took place. It is feasible that the dust and noise levels will be higher in an enclosed operating environment of different construction. In addition, other areas of concern found were arm-hand vibration, whole-body, ergonomics, heat stress, tripping hazards, electrical hazards, machine guarding, and lockout/tagout

  19. Survival significance of epidermal growth factor receptor tyrosine kinase inhibitors and current staging system for survival after recurrence in patients with completely resected lung adenocarcinoma

    Directory of Open Access Journals (Sweden)

    Saji H

    2017-08-01

    Full Text Available Hisashi Saji,1,2 Hiroki Sakai,1 Hiroyuki Kimura,1 Tomoyuki Miyazawa,1 Hideki Marushima,1 Haruhiko Nakamura1 1Department of Chest Surgery, St Marianna University School of Medicine, Miyamae-ku, Kawasaki, Kanagawa, Japan; 2Department of Thoracic Surgery, Tokyo Medical University, Shinjuku-ku, Tokyo, Japan Objective: We previously reported that the staging system and epidermal growth factor receptor (EGFR mutation status are key factors for treatment strategy and predicting survival. However, the significance of these factors as predictors of overall survival (OS and postoperative recurrence survival (PRS has not been sufficiently elucidated. The objective here was to investigate EGFR mutation status and p-stage, which affect PRS and OS in patients with completely resected lung adenocarcinoma, using a different database.Patients and methods: We retrospectively reviewed 56 consecutive lung adenocarcinoma patients with disease recurrence in St. Marianna University Hospital between January 2010 and December 2014.Results: EGFR mutants (M were detected in 16/56 patients (29%. The patients with EGFR M had a better OS than those with EGFR wild-type (WT status (5-year survival: 50.3% vs 43.1, P=0.133. There was no significant difference in the 3-year recurrence-free survival rate between patients with M and WT (6.3% vs 7.7%, P=0.656, and the patients with EGFR M had a significantly better 3-year PRS than those with WT (77.4% vs 51.7%, P=0.033. The 3-year PRS rate for patients with M/pathologic stage (p-stage I–II (87.5% was better than that for patients with M/p-stage III (60.0%, WT/p-stage I–II (52.7%, and WT/p-stage III (43.8%. There was a significant difference between patients with M/p-stage I and WT/p-stage I–II or WT/p-stage III (P=0.021 and 0.030, respectively. During the study period, of the 16 patients with mutants, 12 patients (75% received EGFR-tyrosine kinase inhibitor (TKI therapy and among the 40 patients with WT, no patient received

  20. Hindbrain A2 noradrenergic neuron adenosine 5'-monophosphate-activated protein kinase activation, upstream kinase/phosphorylase protein expression, and receptivity to hormone and fuel reporters of short-term food deprivation are regulated by estradiol.

    Science.gov (United States)

    Briski, Karen P; Alenazi, Fahaad S H; Shakya, Manita; Sylvester, Paul W

    2017-07-01

    Estradiol (E) mitigates acute and postacute adverse effects of 12 hr-food deprivation (FD) on energy balance. Hindbrain 5'-monophosphate-activated protein kinase (AMPK) regulates hyperphagic and hypothalamic metabolic neuropeptide and norepinephrine responses to FD in an E-dependent manner. Energy-state information from AMPK-expressing hindbrain A2 noradrenergic neurons shapes neural responses to metabolic imbalance. Here we investigate the hypothesis that FD causes divergent changes in A2 AMPK activity in E- vs. oil (O)-implanted ovariectomized female rats, alongside dissimilar adjustments in circulating metabolic fuel (glucose, free fatty acids [FFA]) and energy deficit-sensitive hormone (corticosterone, glucagon, leptin) levels. FD decreased blood glucose in oil (O)- but not E-implanted ovariectomized female rats and elevated and reduced glucagon levels in O and E, respectively. FD decreased circulating leptin in O and E, but increased corticosterone and FFA concentrations in E only. Western blot analysis of laser-microdissected A2 neurons showed that glucocorticoid receptor type II and very-long-chain acyl-CoA synthetase 3 protein profiles were amplified in FD/E vs. FD/O. A2 total AMPK protein was elevated without change in activity in FD/O, whereas FD/E exhibited increased AMPK activation along with decreased upstream phosphatase expression. The catecholamine biosynthetic enzyme dopamine-β-hydroxylase (DβH) was increased in FD/O but not FD/E A2 cells. The data show discordance between A2 AMPK activation and glycemic responses to FD; sensor activity was refractory to glucose decrements in FD/O but augmented in FD/E despite stabilized glucose and elevated FFA levels. E-dependent amplification of AMPK activity may reflect adaptive conversion to fatty acid oxidation and/or glucocorticoid stimulation. FD augmentation of A2 DβH protein profiles in FD/O but not FD/E animals suggests that FD may correspondingly regulate NE synthesis vs. metabolism/release in the

  1. Transactive Campus Energy Systems: Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Katipamula, Srinivas [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Corbin, Charles D. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Haack, Jereme N. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Hao, He [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Kim, Woohyun [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Hostick, Donna J. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Akyol, Bora A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Allwardt, Craig H. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Carpenter, Brandon J. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Huang, Sen [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Liu, Guopeng [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Lutes, Robert G. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Makhmalbaf, Atefe [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Mendon, Vrushali V. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Ngo, Hung [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Somasundaram, Sriram [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Underhill, Ronald M. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Zhao, Mingjie [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2017-09-26

    Transactive energy refers to the combination of economic and control techniques to improve grid reliability and efficiency. The fundamental purpose of transactive energy management is to seamlessly coordinate the operation of large numbers of new intelligent assets—such as distributed solar, energy storage and responsive building loads—to provide the flexibility needed to operate the power grid reliably and at minimum cost, particularly one filled with intermittent renewable generation such as the Pacific Northwest. It addresses the key challenge of providing smooth, stable, and predictable “control” of these assets, despite the fact that most are neither owned nor directly controlled by the power grid. The Clean Energy and Transactive Campus (CETC) work described in this report was done as part of a Cooperative Research and Development Agreement (CRADA) between the U.S. Department of Energy’s Pacific Northwest National Laboratory (PNNL) and the Washington State Department of Commerce (Commerce) through the Clean Energy Fund (CEF). The project team consisted of PNNL, the University of Washington (UW) and Washington State University (WSU), to connect the PNNL, UW, and WSU campuses to form a multi-campus testbed for transaction-based energy management—transactive—solutions. Building on the foundational transactive system established by the Pacific Northwest Smart Grid Demonstration (PNWSGD), the purpose of the project was to construct the testbed as both a regional flexibility resource and as a platform for research and development (R&D) on buildings/grid integration and information-based energy efficiency. This report provides a summary of the various tasks performed under the CRADA.

  2. The evaluation of a web-based incident reporting system.

    Science.gov (United States)

    Kuo, Ya-Hui; Lee, Ting-Ting; Mills, Mary Etta; Lin, Kuan-Chia

    2012-07-01

    A Web-based reporting system is essential to report incident events anonymously and confidentially. The purpose of this study was to evaluate a Web-based reporting system in Taiwan. User satisfaction and impact of system use were evaluated through a survey answered by 249 nurses. Incident events reported in paper and electronic systems were collected for comparison purposes. Study variables included system user satisfaction, willingness to report, number of reports, severity of the events, and efficiency of the reporting process. Results revealed that senior nurses were less willing to report events, nurses on internal medicine units had higher satisfaction than others, and lowest satisfaction was related to the time it took to file a report. In addition, the Web-based reporting system was used more often than the paper system. The percentages of events reported were significantly higher in the Web-based system in laboratory, environment/device, and incidents occurring in other units, whereas the proportions of reports involving bedsores and dislocation of endotracheal tubes were decreased. Finally, moderate injury event reporting decreased, whereas minor or minimal injury event reporting increased. The study recommends that the data entry process be simplified and the network system be improved to increase user satisfaction and reporting rates.

  3. Buried waste containment system materials. Final Report

    International Nuclear Information System (INIS)

    Weidner, J.R.; Shaw, P.G.

    1997-10-01

    This report describes the results of a test program to validate the application of a latex-modified cement formulation for use with the Buried Waste Containment System (BWCS) process during a proof of principle (POP) demonstration. The test program included three objectives. One objective was to validate the barrier material mix formulation to be used with the BWCS equipment. A basic mix formula for initial trials was supplied by the cement and latex vendors. The suitability of the material for BWCS application was verified by laboratory testing at the Idaho National Engineering and Environmental Laboratory (INEEL). A second objective was to determine if the POP BWCS material emplacement process adversely affected the barrier material properties. This objective was met by measuring and comparing properties of material prepared in the INEEL Materials Testing Laboratory (MTL) with identical properties of material produced by the BWCS field tests. These measurements included hydraulic conductivity to determine if the material met the US Environmental Protection Agency (EPA) requirements for barriers used for hazardous waste sites, petrographic analysis to allow an assessment of barrier material separation and segregation during emplacement, and a set of mechanical property tests typical of concrete characterization. The third objective was to measure the hydraulic properties of barrier material containing a stop-start joint to determine if such a feature would meet the EPA requirements for hazardous waste site barriers

  4. Pyruvate kinase blood test

    Science.gov (United States)

    ... medlineplus.gov/ency/article/003357.htm Pyruvate kinase blood test To use the sharing features on this page, ... energy when oxygen levels are low. How the Test is Performed A blood sample is needed. In the laboratory, white blood ...

  5. Licensee Event Report system: description of system and guidelines for reporting

    International Nuclear Information System (INIS)

    Hebdon, F.J.

    1983-09-01

    On July 26, 1983, the Commission published in the Federal Register a final rule (10 CFR 50.73) that modifies and codifies the Licensee Event Report (LER) system. The rule becomes effective on January 1, 1984. This NUREG provides supporting information and guidance that will be of interest to persons responsible for the preparation and review of LERs. The information contained in this NUREG includes: (1) a brief description of how LERs are analyzed by the NRC; (2) a restatement of the guidance contained in the Statement of Consideration that accompanied the publication of the LER rule; (3) a set of examples of potentially reportable events with staff comments on the actual reportability of each event; (4) guidance on how to prepare an LER, including the LER forms; and (5) guidance on submittal of LERs

  6. 20 CFR 637.300 - Management systems, reporting and recordkeeping.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Management systems, reporting and... Standards and Procedures § 637.300 Management systems, reporting and recordkeeping. (a) The Governor shall ensure that the State's financial management system and recordkeeping system comply with subpart D of...

  7. Structural basis for substrate specificities of cellular deoxyribonucleoside kinases

    DEFF Research Database (Denmark)

    Johansson, K.; Ramaswamy, S.; Ljungcrantz, C.

    2001-01-01

    Deoxyribonucleoside kinases phosphorylate deoxyribonucleosides and activate a number of medically important nucleoside analogs. Here we report the structure of the Drosophila deoxyribonucleoside kinase with deoxycytidine bound at the nucleoside binding site and that of the human deoxyguanosine ki......; this is apparently due to the presence of Arg 118, which provides favorable hydrogen bonding interactions with the substrate. The two new structures provide an explanation for the substrate specificity of cellular deoxyribonucleoside kinases....

  8. A brief report on mets system

    Digital Repository Service at National Institute of Oceanography (India)

    Fernandes, W.A.

    Mets system is basically a gas monitoring system, used for the detection of underwater gas. The system consists of a sensor, datalogger and energy module. The sensor works on the diffusion techniques. The system can be deployed to a water depth...

  9. Liquid waste treatment system. Final report

    International Nuclear Information System (INIS)

    Baker, M.N.; Houston, H.M.

    1999-01-01

    Pretreatment of high-level liquid radioactive waste (HLW) at the West Valley Demonstration Project (WVDP) involved three distinct processing operations: decontamination of liquid HLW in the Supernatant Treatment System (STS); volume reduction of decontaminated liquid in the Liquid Waste Treatment System (LWTS); and encapsulation of resulting concentrates into an approved cement waste form in the Cement Solidification System (CSS). Together, these systems and operations made up the Integrated Radwaste Treatment System (IRTS)

  10. Genetic Variants of IκB Kinase β (IKBKB and Polymerase β (POLB Were Not Associated with Systemic Lupus Erythematosus Risk in a Chinese Han Population.

    Directory of Open Access Journals (Sweden)

    Yuan Li

    Full Text Available A previous large-scale replication study validation of a genome wide association study (GWAS identified IκB kinase β (IKBKB single nucleotide polymorphisms (SNPs as a risk factor associated with systemic lupus erythematosus (SLE in a Chinese Han population. IKBKB SNPs were associated with polymerase β (POLB SNPs and reduced POLB expression, and this was proposed to be an underlying cause of human SLE development. In the current case-control study, we evaluated IKBKB (rs12676482 and rs2272733 and POLB (rs3136717 and rs3136744 SNPs in 946 SLE patients and 961 healthy controls. We investigated the possible association of these four SNPs with SLE in a Chinese Han population using the polymerase chain reaction-ligation detection reaction (PCR-LDR technique. The differences in the frequencies of the four SNP alleles and the genotypes and haplotypes of the POLB polymorphisms were statistically insignificant when the SLE patients were compared with the controls in the Chinese Han population enrolled in this study (all, p ˃ 0.05. Furthermore, no associations were detected using different genetic models (additive, dominant, and recessive; all, p ˃ 0.05. Our findings indicate that the IKBKB (rs12676482 and rs2272733 and POLB (rs3136717, rs3136744 SNPs confer no genetic predisposition to SLE risk in this Chinese Han population.

  11. The experimental study of reporter probe 131I-FIAU in neonatal cardiac myocytes after transfer of herpes simplex virus type 1 thymidine kinase reporter gene by different vectors

    International Nuclear Information System (INIS)

    Yin Xiaohua; Lan Xiaoli; Wang Ruihua; Liu Ying; Zhang Yongxue

    2009-01-01

    Objective: Reporter gene imaging is a promising approach for noninvasive monitoring of cardiac gene therapy. In the present study, the recombinant plasmid and adenoviral vector carrying reporter gene. herpes simplex virus type 1 thymidine kinase (HSV1-tk), were constructed and transferred into nee-natal cardiac myocytes, and a series of in vitro studies were carried out on the cells transferred to evaluate the uptake of radiolabeled reporter probe and to compare both vectors for cardiac reporter gene imaging. Methods: Neonatal cardiac myocytes were obtained from rat heart by single collagenase digestion. HSVI-tk. chosen as the reporter gene.was inserted into adenovirus vector (Ad5-tk) and plasmid (pDC316-tk), thus it could be transferred into neonatal cardiac myocytes. Recombinant adenovirus containing enhanced green fluorescent protein (Ad5-EGFP) was used as control. Recombinant plasmid was coated with lipofectamine TM 2000 (pDC316-tk/lipoplex). The specific reporter probe of HSV1-tk, 2'-fluoro-2'-deoxy-l-β-D-arabinofuranosyl-uracil (FAU), was labeled with 131 I by solid phase oxidation with lodogen. Product wag purified on a reverse. phase Sep-Pak C18 column and the radiochemical purity wag then assessed. The accumulation of it in the transferred cardiac myocytes wag detected as uptake rate. Furthermore, mRNA expression of HSV1-tk was detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), while its protein expression wag located by immunocytochemistry. Results: FAU could be labeled with 131 I and the labeling efficiency was (53.82 ±2.05)%. The radiochemical purity was (94.85 ± 1.76)% after purification, and it kept stable in vitro for at least 24h. Time-dependent increase of the ac- cumulation of 131 I-FIAU was observed in both Ad5-tk group and pDC316-tk/lipoplex group. and the highest uptake rate occurred at 5h, with peak values of (12.55 ± 0.37)% and (2.09 ± 0.34)% respectively. However, it also indicated that greater

  12. Gas characterization system software acceptance test report

    International Nuclear Information System (INIS)

    Vo, C.V.

    1996-01-01

    This document details the results of software acceptance testing of gas characterization systems. The gas characterization systems will be used to monitor the vapor spaces of waste tanks known to contain measurable concentrations of flammable gases

  13. SIMS Prototype System 4: performance test report

    Energy Technology Data Exchange (ETDEWEB)

    1978-10-09

    The results obtained during testing of a self-contained, preassembled air type solar system, designed for installation remote from the dwelling, to provide space heating and hot water are presented. Data analysis is included which documents the system performance and verifies the suitability of SIMS Prototype System 4 for field installation.

  14. Advanced ultrasonic field system: a status report

    International Nuclear Information System (INIS)

    Mikesell, C.R.; Beller, L.S.

    1984-02-01

    An advanced ultrasonic system was developed to obtain highly reproducible inspection data and to overcome certain limitations encountered with the manual scanning method. Experience from field operations from 1976 through 1980 is discussed. The scope includes a description of the computer controlled system, personnel training, inservice inspections, data analysis, and current upgrading of the system

  15. Synthesis and Physicochemical Characterization of D-Tagatose-1-Phosphate: The Substrate of the Tagatose-1-Phosphate Kinase in the Phosphotransferase System-Mediated D-Tagatose Catabolic Pathway of Bacillus licheniformis.

    Science.gov (United States)

    Van der Heiden, Edwige; Delmarcelle, Michaël; Simon, Patricia; Counson, Melody; Galleni, Moreno; Freedberg, Darón I; Thompson, John; Joris, Bernard; Battistel, Marcos D

    2015-01-01

    We report the first enzymatic synthesis of D-tagatose-1-phosphate (Tag-1P) by the multicomponent phosphoenolpyruvate:sugar phosphotransferase system (PEP-PTS) present in tagatose-grown cells of Klebsiella pneumoniae. Physicochemical characterization by (31)P and (1)H nuclear magnetic resonance spectroscopy reveals that, in solution, this derivative is primarily in the pyranose form. Tag-1P was used to characterize the putative tagatose-1-phosphate kinase (TagK) of the Bacillus licheniformis PTS-mediated D-tagatose catabolic pathway (Bli-TagP). For this purpose, a soluble protein fusion was obtained with the 6 His-tagged trigger factor (TF(His6)) of Escherichia coli. The active fusion enzyme was named TagK-TF(His6). Tag-1P and D-fructose-1-phosphate are substrates for the TagK-TF(His6) enzyme, whereas the isomeric derivatives D-tagatose-6-phosphate and D-fructose-6-phosphate are inhibitors. Studies of catalytic efficiency (kcat/Km) reveal that the enzyme specificity is markedly in favor of Tag-1P as the substrate. Importantly, we show in vivo that the transfer of the phosphate moiety from PEP to the B. licheniformis tagatose-specific Enzyme II in E. coli is inefficient. The capability of the PTS general cytoplasmic components of B. subtilis, HPr and Enzyme I to restore the phosphate transfer is demonstrated. © 2015 S. Karger AG, Basel.

  16. Advances in study of molecular imaging reporte gene systems

    International Nuclear Information System (INIS)

    Wu Tao; An Rui

    2010-01-01

    The use of molecular imaging reporter gene systems has allowed gene therapy to move from the laboratory to the clinical application, which provides methodology to monitor the expression of therapeutic gene noninvasively and achieve quantitative outcome in vivo. Recently, the radionuclide reporter gene still is the focus many studies, but MRI and optical reporter gene have gradually played a important part in reporter gene systems. On the basis of combination of multi-subject, for example applied chemistry and molecular biology, more and more new modified reporter genes and molecular probes have spread out. This paper mainly introduces the advantages and disadvantages of reporter gene system and development trends. (authors)

  17. The Pim kinases: new targets for drug development.

    Science.gov (United States)

    Swords, Ronan; Kelly, Kevin; Carew, Jennifer; Nawrocki, Stefan; Mahalingam, Devalingam; Sarantopoulos, John; Bearss, David; Giles, Francis

    2011-12-01

    The three Pim kinases are a small family of serine/threonine kinases regulating several signaling pathways that are fundamental to cancer development and progression. They were first recognized as pro-viral integration sites for the Moloney Murine Leukemia virus. Unlike other kinases, they possess a hinge region which creates a unique binding pocket for ATP. Absence of a regulatory domain means that these proteins are constitutively active once transcribed. Pim kinases are critical downstream effectors of the ABL (ableson), JAK2 (janus kinase 2), and Flt-3 (FMS related tyrosine kinase 1) oncogenes and are required by them to drive tumorigenesis. Recent investigations have established that the Pim kinases function as effective inhibitors of apoptosis and when overexpressed, produce resistance to the mTOR (mammalian target of rapamycin) inhibitor, rapamycin . Overexpression of the PIM kinases has been reported in several hematological and solid tumors (PIM 1), myeloma, lymphoma, leukemia (PIM 2) and adenocarcinomas (PIM 3). As such, the Pim kinases are a very attractive target for pharmacological inhibition in cancer therapy. Novel small molecule inhibitors of the human Pim kinases have been designed and are currently undergoing preclinical evaluation.

  18. Technical report for effective estimation and improvement of quality system

    International Nuclear Information System (INIS)

    Kim, Kwan Hyun

    2000-06-01

    This technical report provides the methods on how to improve the Quality System, in R and D part. This report applies on the quality assurance(QA) programmes of the design, fabrication in nuclear projects. The organization having overall responsibility for the nuclear power item design, preservation, fabrication shall be described in this report in each stage of improvement of QA systems

  19. Use of mathematics to guide target selection in systems pharmacology; application to receptor tyrosine kinase (RTK) pathways.

    Science.gov (United States)

    Benson, Neil; van der Graaf, Piet H; Peletier, Lambertus A

    2017-11-15

    A key element of the drug discovery process is target selection. Although the topic is subject to much discussion and experimental effort, there are no defined quantitative rules around optimal selection. Often 'rules of thumb', that have not been subject to rigorous exploration, are used. In this paper we explore the 'rule of thumb' notion that the molecule that initiates a pathway signal is the optimal target. Given the multi-factorial and complex nature of this question, we have simplified an example pathway to its logical minimum of two steps and used a mathematical model of this to explore the different options in the context of typical small and large molecule drugs. In this paper, we report the conclusions of our analysis and describe the analysis tool and methods used. These provide a platform to enable a more extensive enquiry into this important topic. Copyright © 2017 Elsevier B.V. All rights reserved.

  20. Validation of the digital PCR system in tyrosine kinase inhibitor-resistant EGFR mutant non-small-cell lung cancer.

    Science.gov (United States)

    Masago, Katsuhiro; Fujita, Shiro; Hata, Akito; Okuda, Chiyuki; Yoshizumi, Yuko; Kaji, Reiko; Katakami, Nobuyuki; Hirata, Yukio; Yatabe, Yasushi

    2018-03-01

    The aim of this study was to compare the accuracy of the QuantStudio 3D Digital polymerase chain reaction (dPCR) system and a PCR-based next generation sequencing (NGS) system for detecting a secondary mutation in the epidermal growth factor receptor (EGFR) gene T790M in non-small cell lung cancer (NSCLC) patients previously diagnosed with EGFR-activating mutations. Twenty-five patients with NSCLC previously treated with EGFR-TKIs were examined. The patients were treated daily with either erlotinib or gefitinib. New biopsies, followed by DNA sequencing on an Ion Torrent systems using the Ion Torrent AmpliSeq Cancer Hotspot Panel and dPCR were performed. A comparison of NGS, sensitive PCR, and dPCR revealed that the sensitivities of NGS and dPCR were similar in this study. As well, T790M was detected in as low as about 5% of mutant allelic frequencies, which represented 5% of the total reads on site mapped reads in NGS and greater than 5% of the dPCR reads, which represented mutant and wild type copies. The strategy in which NGS sequencing is followed by revealed acquired mutation with dPCR may be a reasonable one. We demonstrated the utility of combining NGS and dPCR as a tool for monitoring T790M. NGS and dPCR with formalin-fixed paraffin-embedded (FFPE) specimens might become a standard genomic test for exploring acquired resistance to targeted molecular medicines. © 2018 Japanese Society of Pathology and John Wiley & Sons Australia, Ltd.

  1. Hawaii DAR Fisherman Reporting System Data (Catch)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains the catch data from fishers who take marine life for commercial purposes and report their catch, effort, and sales on a commercial catch...

  2. Technical study report on reprocessing system

    International Nuclear Information System (INIS)

    Tanaka, Hiroshi; Kasai, Yoshimitsu; Kawamura, Fumio

    2000-07-01

    The feasibility studies on commercialized fast breeder reactor (FBR) recycle system, in which reactor system and recycle technologies are related on consideration, are performed considering the attainable perspectives for the followings: ensuring safety, economic competitiveness to future LWRs, efficient utilization of resources, reduction of environmental burden, and enhancement of nuclear non-proliferation. In the studies of Reprocessing system of FBR, evaluation was made for the non-aqueous reprocessing of pyrochemical and fluoride volatility process and also for the aqueous reprocessing with a view to streamlining. As a result, it was estimated that each system has prospects of coming into practical use in terms of technique. In the economical efficiency assessment, it was estimated to have economic competitiveness to future LWRs. And the technical research items of each system are picked out. Hereafter, more detail design study will be performed for each system. (author)

  3. Electrical System Technology Working Group (WG) Report

    Science.gov (United States)

    Silverman, S.; Ford, F. E.

    1984-01-01

    The technology needs for space power systems (military, public, commercial) were assessed for the period 1995 to 2005 in the area of power management and distribution, components, circuits, subsystems, controls and autonomy, modeling and simulation. There was general agreement that the military requirements for pulse power would be the dominant factor in the growth of power systems. However, the growth of conventional power to the 100 to 250kw range would be in the public sector, with low Earth orbit needs being the driver toward large 100kw systems. An overall philosophy for large power system development is also described.

  4. Phosphorylation of the Yeast Choline Kinase by Protein Kinase C

    Science.gov (United States)

    Choi, Mal-Gi; Kurnov, Vladlen; Kersting, Michael C.; Sreenivas, Avula; Carman, George M.

    2005-01-01

    The Saccharomyces cerevisiae CKI1-encoded choline kinase catalyzes the committed step in phosphatidylcholine synthesis via the Kennedy pathway. The enzyme is phosphorylated on multiple serine residues, and some of this phosphorylation is mediated by protein kinase A. In this work, we examined the hypothesis that choline kinase is also phosphorylated by protein kinase C. Using choline kinase as a substrate, protein kinase C activity was dose- and time-dependent, and dependent on the concentrations of choline kinase (Km = 27 μg/ml) and ATP (Km = 15 μM). This phosphorylation, which occurred on a serine residue, was accompanied by a 1.6-fold stimulation of choline kinase activity. The synthetic peptide SRSSS25QRRHS (Vmax/Km = 17.5 mM-1 μmol min-1 mg-1) that contains the protein kinase C motif for Ser25 was a substrate for protein kinase C. A Ser25 to Ala (S25A) mutation in choline kinase resulted in a 60% decrease in protein kinase C phosphorylation of the enzyme. Phosphopeptide mapping analysis of the S25A mutant enzyme confirmed that Ser25 was a protein kinase C target site. In vivo, the S25A mutation correlated with a decrease (55%) in phosphatidylcholine synthesis via the Kennedy pathway whereas an S25D phosphorylation site mimic correlated with an increase (44%) in phosphatidylcholine synthesis. Whereas the S25A (protein kinase C site) mutation did not affect the phosphorylation of choline kinase by protein kinase A, the S30A (protein kinase A site) mutation caused a 46% reduction in enzyme phosphorylation by protein kinase C. A choline kinase synthetic peptide (SQRRHS30LTRQ) containing Ser30 was a substrate (Vmax/Km = 3.0 mM−1 μmol min−1 mg−1) for protein kinase C. Comparison of phosphopeptide maps of the wild type and S30A mutant choline kinase enzymes phosphorylated by protein kinase C confirmed that Ser30 was also a target site for protein kinase C. PMID:15919656

  5. A new safety event reporting system improves physician reporting in the surgical intensive care unit.

    Science.gov (United States)

    Schuerer, Douglas J E; Nast, Patricia A; Harris, Carolyn B; Krauss, Melissa J; Jones, Rebecca M; Boyle, Walter A; Buchman, Timothy G; Coopersmith, Craig M; Dunagan, W Claiborne; Fraser, Victoria J

    2006-06-01

    Medical errors are common, and physicians have notably been poor medical error reporters. In the SICU, reporting was generally poor and reporting by physicians was virtually nonexistent. This study was designed to observe changes in error reporting in an SICU when a new card-based system (SAFE) was introduced. Before implementation of the SAFE reporting system, education was given to all SICU healthcare providers. The SAFE system was introduced into the SICU for a 9-month period from March 2003 through November 2003, to replace an underused online system. Data were collected from the SAFE card reports and the online reporting systems during introduction, removal, and reimplementation of these cards. Reporting rates were calculated as number of reported events per 1,000 patient days. Reporting rates increased from 19 to 51 reports per 1,000 patient days after the SAFE cards were introduced into the ICU (preporting increased most, rising from 0.3 to 5.8 reports per 1,000 patient days; nursing reporting also increased from 18 to 39 reports per 1,000 patient days (both preporting declined to 0 reports per 1,000 patient days (p=0.01) and rose to 8.1 (p=0.001) when the cards were returned, similar to nursing results. A higher proportion of physician reports were events that caused harm compared with no effect (p reporting system, combined with appropriate education, improved overall reporting in the SICU, especially among physician providers. Nurses were more likely to use reporting systems than were physicians. Physician reports were more likely to be of events that caused harm.

  6. CDC WONDER: Vaccine Adverse Event Reporting System (VAERS)

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Vaccine Adverse Event Reporting System (VAERS) online database on CDC WONDER provides counts and percentages of adverse event case reports after vaccination, by...

  7. FDA Adverse Event Reporting System (FAERS): Latest Quartely Data Files

    Data.gov (United States)

    U.S. Department of Health & Human Services — The FDA Adverse Event Reporting System (FAERS) is a database that contains information on adverse event and medication error reports submitted to FDA. The database...

  8. Development of precast bridge deck overhang system : technical report.

    Science.gov (United States)

    2011-07-01

    The implementation of full-depth, precast overhang panel systems has the potential to improve constructability, : productivity, and make bridges more economical. Initial testing and analyses reported in the 0-6100-2 report resulted in : a design that...

  9. Impaired c-Fos and polo-like kinase 2 induction in the limbic system of fear-conditioned α-synuclein transgenic mice.

    Directory of Open Access Journals (Sweden)

    Heinrich Schell

    Full Text Available α-Synuclein (αSYN is genetically and neuropathologically linked to a spectrum of neurodegenerative diseases including Parkinson's disease, dementia with Lewy bodies, and related disorders. Cognitive impairment is recapitulated in several αSYN transgenic mouse lines. However, the mechanisms of dysfunction in affected neurons are largely unknown. Here we measured neuronal activity induced gene products in the limbic system of αSYN transgenic mice upon fear conditioning (FC. Induction of the synaptic plasticity marker c-Fos was significantly reduced in the amygdala and hippocampus of (Thy1-h[A30P]αSYN transgenic mice in an age-dependent manner. Similarly, the neuronal activity inducible polo-like kinase 2 (Plk2 that can phosphorylate αSYN at the pathological site serine-129 was up-regulated in both brain regions upon FC. Plk2 inductions were also significantly impaired in aged (Thy1-h[A30P]αSYN transgenic mice, both in the amygdala and hippocampus. Plk2 inductions in the amygdala after FC were paralleled by a small but significant increase in the number of neuronal cell bodies immunopositive for serine-129 phosphorylated αSYN in young but not aged (Thy1-h[A30P]αSYN transgenic mice. In addition, we observed in the aged hippocampus a distinct type of apparently unmodified transgenic αSYN profiles resembling synaptic accumulations of αSYN. Thus, the cognitive decline observed in aged αSYN transgenic mice might be due to impairment of neurotransmission and synaptic plasticity in the limbic system by distinct αSYN species.

  10. FY2015 Vehicle Systems Annual Progress Report

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2016-01-31

    The Vehicle Systems research and development (R&D) subprogram within the DOE Vehicle Technologies Office (VTO) provides support and guidance for many cutting-edge automotive technologies under development. Research focuses on addressing critical barriers to advancing light-, medium-, and heavy-duty vehicle systems to help maximize the number of electric miles driven and increase the energy efficiency of transportation vehicles.

  11. FY2016 Vehicle Systems Annual Progress Report

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2017-10-31

    Vehicle Systems is concerned with advancing light-, medium-, and heavy-duty (HD) vehicle systems to support DOE’s goals of developing technologies for the U.S. transportation sector that enhance national energy security,increase U.S. competitiveness in the global economy, and support improvement of U.S. transportation and energy infrastructure.

  12. Photovoltaic systems concept study. Final report

    Energy Technology Data Exchange (ETDEWEB)

    1977-04-01

    The work performed in the conceptual design and systems analysis of three sizes of photovoltaic solar electric power systems is contained in five volumes consisting of nine sections plus appendices. Separate abstracts were prepared for the two sections in this volume. (MHR)

  13. SIMS prototype system 4 - performance test report

    Science.gov (United States)

    1978-01-01

    A self-contained, preassembled air type solar system, designed for installation remote from the dwelling, to provide space heating and hot water was evaluated. Data analysis is included which documents the system performance and verifies its suitability for field installation.

  14. Report on the SLC control system

    International Nuclear Information System (INIS)

    Phinney, N.

    1985-05-01

    The SLC control system is based on a VAX 11/780 Host computer with approximately 50 microprocessor clusters which provide distributed intelligence and control of all CAMAC interface modules. This paper will present an overview of the system including current status and a description of the software architecture and communication protocols. 8 refs

  15. Preliminary Systems Design Study assessment report

    International Nuclear Information System (INIS)

    Mayberry, J.L.; Feizollahi, F.; Del Signore, J.C.

    1992-01-01

    The System Design Study (SDS), part of the Waste Technology Development Department at the Idaho National Engineering Laboratory (INEL), examined techniques for the remediation of hazardous and transuranic waste stored at Radioactive Waste Management Complex's Subsurface Disposal Area at the INEL. Using specific technologies, system concepts for treating the buried waste and the surrounding contaminated soil were evaluated. Evaluation included implementability, effectiveness, and cost. The SDS resulted in the development of technology requirements including demonstration, testing, and evaluation activities needed for implementing each concept. This volume contains introduction section containing a brief SDS background and lists the general assumptions and considerations used during the development of the system concepts. The introduction section is followed by sections describing two system concepts that produce a waste form in compliance with the Waste Isolation Pilot Plant (WIPP) Waste Acceptance Criteria (WAC) and transportation package (TRAMPAC) requirements. This system concept category is referred to as Waste Form 4, ''WIPP and TRAMPAC Acceptable.'' The following two system concepts are under this category: Sort, Treat, and Repackage System (4-BE-2); Volume Reduction and Packaging System (4-BE-4)

  16. Enterococcus faecalis phosphomevalonate kinase

    Science.gov (United States)

    Doun, Stephanie S.; Burgner, John W.; Briggs, Scott D.; Rodwell, Victor W.

    2005-01-01

    The six enzymes of the mevalonate pathway of isopentenyl diphosphate biosynthesis represent potential for addressing a pressing human health concern, the development of antibiotics against resistant strains of the Gram-positive streptococci. We previously characterized the first four of the mevalonate pathway enzymes of Enterococcus faecalis, and here characterize the fifth, phosphomevalonate kinase (E.C. 2.7.4.2). E. faecalis genomic DNA and the polymerase chain reaction were used to clone DNA thought to encode phosphomevalonate kinase into pET28b(+). Double-stranded DNA sequencing verified the sequence of the recombinant gene. The encoded N-terminal hexahistidine-tagged protein was expressed in Escherichia coli with induction by isopropylthiogalactoside and purified by Ni++ affinity chromatography, yield 20 mg protein per liter. Analysis of the purified protein by MALDI-TOF mass spectrometry established it as E. faecalis phosphomevalonate kinase. Analytical ultracentrifugation revealed that the kinase exists in solution primarily as a dimer. Assay for phosphomevalonate kinase activity used pyruvate kinase and lactate dehydrogenase to couple the formation of ADP to the oxidation of NADH. Optimal activity occurred at pH 8.0 and at 37°C. The activation energy was ~5.6 kcal/mol. Activity with Mn++, the preferred cation, was optimal at about 4 mM. Relative rates using different phosphoryl donors were 100 (ATP), 3.6 (GTP), 1.6 (TTP), and 0.4 (CTP). Km values were 0.17 mM for ATP and 0.19 mM for (R,S)-5-phosphomevalonate. The specific activity of the purified enzyme was 3.9 μmol substrate converted per minute per milligram protein. Applications to an immobilized enzyme bioreactor and to drug screening and design are discussed. PMID:15802646

  17. ATR kinase regulates its attenuation via PPM1D phosphatase ...

    Indian Academy of Sciences (India)

    In eukaryotes, in response to replication stress, DNA damage response kinase, ATR is activated, whose signalling abrogationleads to cell lethality due to aberrant fork remodelling and excessive origin firing. Here we report that inhibition ofATR kinase activity specifically during replication stress recovery results in persistent ...

  18. Technical study report on fuel fabrication system

    International Nuclear Information System (INIS)

    Kono, Shusaku; Tanaka, Kenya; Ono, Kiyoshi; Iwasa, Katsuyoshi; Hoshino, Yasushi; Shinkai, Yasuo

    2000-07-01

    The feasibility study of FBR and related fuel cycle is performed for developing the FBR recycle system which ensures safety, economic competitiveness, efficient utilization of resources, reduction of environmental burden and enhancement of nuclear non-proliferation under consistency of FBR reactor and fuel cycle systems. In this study, a conceptual design study and system characteristics evaluation are conducted for fuel fabrication systems of pellet process, vibropack process for oxide and nitride fuel and casting process for metal fuel. Technical issues in each process are also extracted. In 1999 fiscal year, a conceptual design study were conducted for the fuel fabrication plants adopting (1) the short pellet process which simplifies the conventional MOX pellet fabrication processes, (2) vibropack processes of aqueous gelation process, improved RIAR process, improved ANL process and fluoride volatility process, (3) casting processes of injection process, centrifuging process. As a result, attainable perspective was obtained for each fuel fabrication system through the evaluation of apparatuses, layout and facility volume, etc. In each fuel fabrication system, technical issues for practical use were made clear. Hereafter, more detailed study will be performed for each system, and research programs for phase II study will be planned. (author)

  19. Proximity sensor system development. CRADA final report

    International Nuclear Information System (INIS)

    Haley, D.C.; Pigoski, T.M.

    1998-01-01

    Lockheed Martin Energy Research Corporation (LMERC) and Merritt Systems, Inc. (MSI) entered into a Cooperative Research and Development Agreement (CRADA) for the development and demonstration of a compact, modular proximity sensing system suitable for application to a wide class of manipulator systems operated in support of environmental restoration and waste management activities. In teleoperated modes, proximity sensing provides the manipulator operator continuous information regarding the proximity of the manipulator to objects in the workspace. In teleoperated and robotic modes, proximity sensing provides added safety through the implementation of active whole arm collision avoidance capabilities. Oak Ridge National Laboratory (ORNL), managed by LMERC for the United States Department of Energy (DOE), has developed an application specific integrated circuit (ASIC) design for the electronics required to support a modular whole arm proximity sensing system based on the use of capacitive sensors developed at Sandia National Laboratories. The use of ASIC technology greatly reduces the size of the electronics required to support the selected sensor types allowing deployment of many small sensor nodes over a large area of the manipulator surface to provide maximum sensor coverage. The ASIC design also provides a communication interface to support sensor commands from and sensor data transmission to a distributed processing system which allows modular implementation and operation of the sensor system. MSI is a commercial small business specializing in proximity sensing systems based upon infrared and acoustic sensors

  20. Proximity sensor system development. CRADA final report

    Energy Technology Data Exchange (ETDEWEB)

    Haley, D.C. [Oak Ridge National Lab., TN (United States); Pigoski, T.M. [Merrit Systems, Inc. (United States)

    1998-01-01

    Lockheed Martin Energy Research Corporation (LMERC) and Merritt Systems, Inc. (MSI) entered into a Cooperative Research and Development Agreement (CRADA) for the development and demonstration of a compact, modular proximity sensing system suitable for application to a wide class of manipulator systems operated in support of environmental restoration and waste management activities. In teleoperated modes, proximity sensing provides the manipulator operator continuous information regarding the proximity of the manipulator to objects in the workspace. In teleoperated and robotic modes, proximity sensing provides added safety through the implementation of active whole arm collision avoidance capabilities. Oak Ridge National Laboratory (ORNL), managed by LMERC for the United States Department of Energy (DOE), has developed an application specific integrated circuit (ASIC) design for the electronics required to support a modular whole arm proximity sensing system based on the use of capacitive sensors developed at Sandia National Laboratories. The use of ASIC technology greatly reduces the size of the electronics required to support the selected sensor types allowing deployment of many small sensor nodes over a large area of the manipulator surface to provide maximum sensor coverage. The ASIC design also provides a communication interface to support sensor commands from and sensor data transmission to a distributed processing system which allows modular implementation and operation of the sensor system. MSI is a commercial small business specializing in proximity sensing systems based upon infrared and acoustic sensors.

  1. A uniform system for mammographic reporting BI-RADS

    International Nuclear Information System (INIS)

    Masroor, I.; Ahmad, M. N.; Sheikh, M. Y.

    2001-01-01

    Breast image reporting and data system (BI-RADS) is a new system of categorizing and reporting mammographs and mammographic findings recommended by American College of Radiology. The importance of BI-RADS and final assessment categories are discussed. The purpose is to introduce the above-mentioned mammographic reporting system so that it becomes a standard terminology among the medical personnel, involved in the diagnosis and management of breast diseases. (author)

  2. 2016 Earth System Grid Federation Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    Williams, Dean N. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2016-05-10

    The Earth System Grid Federation (ESGF) experienced a major setback in June 2015, when it experienced a security incident that brought all systems to a halt for more than half a year. However, federation developers and management committee members turned the incident into an opportunity to dramatically upgrade the system security and functionality and to develop planning and policy documents to guide ESGF evolution and success. Moreover, despite the incident, ESGF developer working teams continue to make strong and significant progress on various enhancement projects that will help ensure ESGF can meet the needs of the climate community in the coming years.

  3. Photovoltaic systems for export application. Informal report

    Energy Technology Data Exchange (ETDEWEB)

    Duffy, J.; Campbell, H.; Sajo, A.; Sanz, E. [Univ. of Lowell, MA (United States)

    1988-01-31

    One approach to improving the competitiveness of photovoltaic systems is the development of designs specifically for export applications. In other words, where is it appropriate in a system design to incorporate components manufactured and/or assembled in the receiving country in order to improve the photovoltaic exports from the US? What appears to be needed is a systematic method of evaluating the potential for export from the US of PV systems for various application in different countries. Development of such a method was the goal of this project.

  4. Isolated systems with wind power. Main report

    DEFF Research Database (Denmark)

    Lundsager, P.; Bindner, Henrik W.; Clausen, Niels-Erik

    2001-01-01

    The overall objective of this research project is to study the development of methods and guidelines rather than "universal solutions" for the use of wind energy in isolated communities. The main specific objective of the project is to develop and present amore unified and generally applicable...... approach for assessing the technical and economical feasibility of isolated power supply systems with wind energy. As a part of the project the following tasks were carried out: Review of literature, fieldmeasurements in Egypt, development of an inventory of small isolated systems, overview of end...... for Isolated Systems with Wind Power, applicable for international organisations such as donoragencies and development banks....

  5. Licensee Event Report system. Description of system and guidelines for reporting. Suppl. 1

    International Nuclear Information System (INIS)

    1984-02-01

    On July 26, 1983, the Commission published in the Federal Register a final rule (10 CFR 50.73) that modified and codified the Licensee Event Report (LER) system. The rule became effective on January 1, 1984. In September 1983, the NRC published NUREG-1022 which provides supporting information and guidance that is of interest to persons responsible for the preparation and review of LERs. The information contained in NUREG-1022 includes: (1) a brief description of how LERs are analyzed by the NRC, (2) a restatement of the guidance contained in the Statement of Consideration that accompanied the publication of the LER rule, (3) a set of examples of potentially reportable events with staff comments on the actual reportability of each event, (4) guidance on how to prepare an LER, including the LER forms, and (5) guidance on submittal of LERs. Subsequently, during the period from October 25, 1983 to November 16, 1983, the NRC staff held five regional meetings to discuss the scope and content of the LER rule with utility and NRC regional representatives. During these meetings numerous questions arose and were answered. This supplement to NUREG-1022 contains a summary of the questions asked and the answers given

  6. Assignment and Correspondence Tracking System - Tactical / Operational Reporting

    Data.gov (United States)

    Social Security Administration — Reporting data store for the Assignment and Correspondence Tracking System (ACT). ACT automates the assignment and tracking of correspondence processing within the...

  7. Country Operational Plan and Reporting System

    Data.gov (United States)

    US Agency for International Development — This web-based information system allows for the annual entry and updating of Emergency Plan COPs, annual and semiannual program results, and budget information by...

  8. Solar radiation alert system : final report.

    Science.gov (United States)

    2009-03-01

    The Solar Radiation Alert (SRA) system continuously evaluates measurements of high-energy protons made by instruments on GOES satellites. If the measurements indicate a substantial elevation of effective dose rates at aircraft flight altitudes, the C...

  9. Hawaii DAR Dealer Reporting System Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — In 2000 January, the Hawaii Division of Aquatic Resources (DAR) implemented a computerized data processing system for fish dealer data collected state-wide. Hawaii...

  10. LumiCal alignment system - Status report

    CERN Document Server

    Daniluk, W.; Lesiak, T.; Moszczyński, A.; Pawlik, B.; Wojtoń, T.; Zawiejski, L.

    2015-01-01

    The paper describes the status of the laser-based alignment-system for the luminosity detector, LumiCal, taking into considerations the conditions of the International Large Detector in the International Linear Collider project. The design of the system comprises two parts: the first one containing semi-transparent silicon sensors used to deliver simultaneous position measurements in the X,Y directions of the monitored object, and the second one in which the interferometric technique, i.e. the Frequency Scanning Interferometry (FSI), is proposed. Two laboratory prototypes for both components of the system were built and the preliminary measurements of the DUT displacements demonstrated their utility in the design of the final alignment system. The alignment of the LumiCal detector will allow us to monitor the detector displacements and possible deformations in its internal structure. Lack of information of the displacements will introduce a systematic effect which will have an impact on the accuracy of the fi...

  11. Water-storage-tube systems. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Hemker, P.

    1981-12-24

    Passive solar collection/storage/distribution systems were surveyed, designed, fabricated, and mechanically and thermally tested. The types studied were clear and opaque fiberglass tubes, metal tubes with plastic liners, and thermosyphoning tubes. (MHR)

  12. Web-based Project Reporting System

    Data.gov (United States)

    US Agency for International Development — Web-PRS is a web-based system that captures financial information and project status information that is sortable by geographical location, pillar, project type and...

  13. PV Systems Reliability Final Technical Report.

    Energy Technology Data Exchange (ETDEWEB)

    Lavrova, Olga [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Flicker, Jack David [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Johnson, Jay [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Armijo, Kenneth Miguel [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Gonzalez, Sigifredo [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Schindelholz, Eric John [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Sorensen, Neil R. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Yang, Benjamin Bing-Yeh [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2015-12-01

    The continued exponential growth of photovoltaic technologies paves a path to a solar-powered world, but requires continued progress toward low-cost, high-reliability, high-performance photovoltaic (PV) systems. High reliability is an essential element in achieving low-cost solar electricity by reducing operation and maintenance (O&M) costs and extending system lifetime and availability, but these attributes are difficult to verify at the time of installation. Utilities, financiers, homeowners, and planners are demanding this information in order to evaluate their financial risk as a prerequisite to large investments. Reliability research and development (R&D) is needed to build market confidence by improving product reliability and by improving predictions of system availability, O&M cost, and lifetime. This project is focused on understanding, predicting, and improving the reliability of PV systems. The two areas being pursued include PV arc-fault and ground fault issues, and inverter reliability.

  14. AN INTERIM REPORT ON SOFT SYSTEMS EVALUATION

    Directory of Open Access Journals (Sweden)

    Viljem Rupnik

    2011-02-01

    Full Text Available As application areas rapidly grow beyond the theoretical framework of fundamental decision theory we are very often temptated to see whether or not soft systems may promise some efficient modelling of real life problems. The pioneering bust towards soft systems methodology has come from the needs of mathematical sociology. Its contemporary definition as well as its applied architecture have been dealt with as in a paper proposed.

  15. Advanced lubrication systems and materials. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Hsu, S.

    1998-05-07

    This report described the work conducted at the National Institute of Standards and Technology under an interagency agreement signed in September 1992 between DOE and NIST for 5 years. The interagency agreement envisions continual funding from DOE to support the development of fuel efficient, low emission engine technologies in terms of lubrication, friction, and wear control encountered in the development of advanced transportation technologies. However, in 1994, the DOE office of transportation technologies was reorganized and the tribology program was dissolved. The work at NIST therefore continued at a low level without further funding from DOE. The work continued to support transportation technologies in the development of fuel efficient, low emission engine development. Under this program, significant progress has been made in advancing the state of the art of lubrication technology for advanced engine research and development. Some of the highlights are: (1) developed an advanced high temperature liquid lubricant capable of sustaining high temperatures in a prototype heat engine; (2) developed a novel liquid lubricant which potentially could lower the emission of heavy duty diesel engines; (3) developed lubricant chemistries for ceramics used in the heat engines; (4) developed application maps for ceramic lubricant chemistry combinations for design purpose; and (5) developed novel test methods to screen lubricant chemistries for automotive air-conditioning compressors lubricated by R-134a (Freon substitute). Most of these findings have been reported to the DOE program office through Argonne National Laboratory who manages the overall program. A list of those reports and a copy of the report submitted to the Argonne National Laboratory is attached in Appendix A. Additional reports have also been submitted separately to DOE program managers. These are attached in Appendix B.

  16. Innovative technology summary report: Pipe Explorertrademark system

    International Nuclear Information System (INIS)

    1996-01-01

    The Pipe Explorertrademark system, developed by Science and Engineering Associates, Inc. (SEA), under contract with the US Department of Energy (DOE) Morgantown Energy Technology Center, has been used to transport various characterizing sensors into piping systems that have been radiologically contaminated. DOE's nuclear facility decommissioning program must characterize radiological contamination inside piping systems before the pipe can be recycled, remediated, or disposed. Historically, this has been attempted using hand-held survey instrumentation, surveying only the accessible exterior portions of pipe systems. Various measuring difficulties, and in some cases, the inability to measure threshold surface contamination values and worker exposure, and physical access constraints have limited the effectiveness of traditional survey approaches. The Pipe Explorertrademark system provides a viable alternative. The heart of the system is an air-tight membrane, which is initially spooled inside a canister. The end of the membrane protrudes out of the canister and attaches to the pipe being inspected. The other end of the tubular membrane is attached to the tether and characterization tools. When the canister is pressurized, the membrane inverts and deploys inside the pipe. The characterization detector and its cabling is attached to the tethered end of the membrane. As the membrane is deployed into the pipe, the detector and its cabling is towed into the pipe inside the protective membrane; measurements are taken from within the protective membrane. Once the survey measurements are completed, the process is reversed to retrieve the characterization tools

  17. Acceptance Test Report for 241-U compressed air system

    International Nuclear Information System (INIS)

    Freeman, R.D.

    1994-01-01

    This Acceptance Test Report (ATR) documents the results of acceptance testing of a newly upgraded compressed air system at 241-U Farm. The system was installed and the test successfully performed under work package 2W-92-01027

  18. Reports of MC and A system design workshop subgroups

    International Nuclear Information System (INIS)

    Hatcher, C.R.

    1984-01-01

    A summary of subgroup reports from the workshop on design of a materials control and accounting system for a low-enrichment fuel fabrication facility is presented. Responses to a MC and A design system questionnaire are also summarized

  19. Equipment performance monitoring in NPP Krsko (Summarized system health report)

    International Nuclear Information System (INIS)

    Djetelic, N.; Cicvaric, D.

    2004-01-01

    Management common goal is safe, reliable, effective, acceptable to public and conservative/cautious operation of NPP Krsko. A set of programs, including Corrective Action Program, Performance Indicators, Operating Experience, Self Assessment and System Health Report, is developed to assist NPP Krsko management in fulfilling those goals. System Health Report is a tool that management can use to quickly assess how selected systems are performing, to determine where additional management attention is required and to determine if appropriate corrective actions have been established. Summarized System Health Report is developed for management's quick overview of systems status, important system malfunctions and problems as well as major changes from previous assessment period. Summarized Report contains nine sections: status difference including brief explanation, selected performance indicators, new equipment problems, functional failures, important problem analyses, action plan for systems with Potential Danger (RED) status, maintenance rule status overview and systems availability (planned and unplanned).(author)

  20. Advanced Quasioptical Launcher System. Final Report

    International Nuclear Information System (INIS)

    Neilson, Jeffrey

    2010-01-01

    This program developed an analytical design tool for designing antenna and mirror systems to convert whispering gallery RF modes to Gaussian or HE11 modes. Whispering gallery modes are generated by gyrotrons used for electron cyclotron heating of fusion plasmas in tokamaks. These modes cannot be easily transmitted and must be converted to free space or waveguide modes compatible with transmission line systems.This program improved the capability of SURF3D/LOT, which was initially developed in a previous SBIR program. This suite of codes revolutionized quasi-optical launcher design, and this code, or equivalent codes, are now used worldwide. This program added functionality to SURF3D/LOT to allow creating of more compact launcher and mirror systems and provide direct coupling to corrugated waveguide within the vacuum envelope of the gyrotron. Analysis was also extended to include full-wave analysis of mirror transmission line systems. The code includes a graphical user interface and is available for advanced design of launcher systems.

  1. NKS MOMS. Final report. [Mobile Measurement Systems

    Energy Technology Data Exchange (ETDEWEB)

    Nilssen, J. [Norwegian Radiation Protection Authority (NRPA) (Norway); Aage, H.K. [Danish Emergency Management Agency (DEMA) (Denmark); Palsson, S.E. [Icelandic Radiation Safety Authority (IRSA) (Iceland)

    2013-02-15

    Mobile car-borne measurement systems are an important asset in early phase emergency response in all Nordic countries. However, through the development of the systems in the different countries, there are considerable differences between the systems developed. This complicates Nordic cooperation and mutual assistance in emergency situations. This project aimed to facilitate harmonization of mobile measurement systems between the Nordic countries. The project focused on harmonizing data formats, information exchange and measurement strategies. Although the work done was funded by each member, the project established a good platform for cooperation which will hopefully continue beyond the scope of the project. A two-day seminar was held in May 2012, where all participants presented the current status (equipment, methods used etc.), in addition to invited speakers presenting development within the field of mobile detection and in situ measurements. Exchange of experiences and information on different measurement systems and practises in use was an important part of the seminar. The seminar was followed up by a small workshop during the REFOX exercise in Lund, Sweden, September 2012. Exchange of measurement data from the exercise was facilitated through a workspace proveded by NRPA as part of the MOMS project. The work done in this project will be presented at the NordEx12 seminar in March 2013. (Author)

  2. Structural Bioinformatics and Protein Docking Analysis of the Molecular Chaperone-Kinase Interactions: Towards Allosteric Inhibition of Protein Kinases by Targeting the Hsp90-Cdc37 Chaperone Machinery

    Directory of Open Access Journals (Sweden)

    Gennady Verkhivker

    2013-11-01

    Full Text Available A fundamental role of the Hsp90-Cdc37 chaperone system in mediating maturation of protein kinase clients and supporting kinase functional activity is essential for the integrity and viability of signaling pathways involved in cell cycle control and organism development. Despite significant advances in understanding structure and function of molecular chaperones, the molecular mechanisms and guiding principles of kinase recruitment to the chaperone system are lacking quantitative characterization. Structural and thermodynamic characterization of Hsp90-Cdc37 binding with protein kinase clients by modern experimental techniques is highly challenging, owing to a transient nature of chaperone-mediated interactions. In this work, we used experimentally-guided protein docking to probe the allosteric nature of the Hsp90-Cdc37 binding with the cyclin-dependent kinase 4 (Cdk4 kinase clients. The results of docking simulations suggest that the kinase recognition and recruitment to the chaperone system may be primarily determined by Cdc37 targeting of the N-terminal kinase lobe. The interactions of Hsp90 with the C-terminal kinase lobe may provide additional “molecular brakes” that can lock (or unlock kinase from the system during client loading (release stages. The results of this study support a central role of the Cdc37 chaperone in recognition and recruitment of the kinase clients. Structural analysis may have useful implications in developing strategies for allosteric inhibition of protein kinases by targeting the Hsp90-Cdc37 chaperone machinery.

  3. WRAP TRUPACT loading systems operational test report

    International Nuclear Information System (INIS)

    DOSRAMOS, E.V.

    1999-01-01

    This Operational Test Report documents the operational testing of the TRUPACT process equipment HNF-3918, Revision 0, TRUPACT Operational Test Procedure. The test accomplished the following: Procedure validation; Facility equipment interface; Facility personnel support; and Subcontractor personnel support interface. Field changes are documented as test exceptions with resolutions. All resolutions are completed or a formal method is identified to track the resolution through to completion

  4. Preliminary systems design study assessment report

    International Nuclear Information System (INIS)

    Mayberry, J.L.; Feizollahi, F.; Del Signore, J.C.

    1992-01-01

    The System Design Study (SDS), part of the Waste Technology Development Department at the Idaho National Engineering Laboratory (INEL), examined techniques available for the remediation of hazardous and transuranic waste stored at the Radioactive Waste Management Complex's Subsurface Disposal Area at the INEL. Using specific technologies, system concepts for treating the buried waste and the surrounding contaminated soil were evaluated. Evaluation included implementability, effectiveness, and cost. The SDS resulted in the development of technology requirements including demonstration, testing, and evaluation activities needed for implementing each. This volume contains the descriptions and other relevant information of the four subsystems required for most of the ex situ processing systems. This volume covers the metal decontamination and sizing subsystem, soils processing subsystem, low-level waste subsystem, and retrieval subsystem

  5. Preliminary Systems Design Study assessment report

    International Nuclear Information System (INIS)

    Mayberry, J.L.; Quapp, W.J.; Feizollahi, F.; Del Signore, J.C.

    1991-07-01

    The System Design Study (SDS), part of the Waste Technology Development Department at Idaho National Engineering Laboratory (INEL), examined techniques available for the remediation of hazardous and transuranic (TRU) waste stored at the Radioactive Waste Management Complex's (RWMC's) Subsurface Disposal Area (SDA) at INEL. Using specific technologies, system concepts for treating the buried waste and the surrounding contaminated soil were evaluated. Evaluation included implementability, effectiveness, and cost. SDS resulted in the development of technology requirements including demonstration, testing and evaluation activities needed for implementing each concept. The SDS results are published in eight volumes. Volume 1 contains an executive summary. The SDS summary and analysis of results are presented in volume 2. Volumes 3 through 7 contain detailed descriptions of twelve system and four subsystem concepts. Volume 8 contains the appendices. 3 figs., 3 tabs

  6. Preliminary Systems Design Study assessment report

    International Nuclear Information System (INIS)

    Mayberry, J.L.; Quapp, W.J.; Feizollahi, F.; Del Signore, J.C.

    1991-07-01

    The System Design Study (SDS), part of the Waste Technology Development Department at the Idaho National Engineering Laboratory (INEL), examined techniques available for the remediation of hazardous and transuranic waste stored at the Radioactive Waste Management Complex's Subsurface Disposal Area at the INEL. Using specific technologies, system concepts for treating the buried waste and the surrounding contaminated soil were evaluated. Evaluation included implementability, effectiveness, and cost. SDS resulted in the development of technology requirements including demonstration, testing, and evaluation activities needed for implementing each concept. The SDS results are published in eight volumes. Volume 1 contains an executive summary. The SDS summary and analysis of results are presented in Volume 2. Volumes 3 through 7 contain detailed descriptions of twelve system and four subsystem concepts. Volume 8 contains the appendixes. 23 refs., 23 figs., 16 tabs

  7. Adenylate Kinase and AMP Signaling Networks: Metabolic Monitoring, Signal Communication and Body Energy Sensing

    Directory of Open Access Journals (Sweden)

    Andre Terzic

    2009-04-01

    Full Text Available Adenylate kinase and downstream AMP signaling is an integrated metabolic monitoring system which reads the cellular energy state in order to tune and report signals to metabolic sensors. A network of adenylate kinase isoforms (AK1-AK7 are distributed throughout intracellular compartments, interstitial space and body fluids to regulate energetic and metabolic signaling circuits, securing efficient cell energy economy, signal communication and stress response. The dynamics of adenylate kinase-catalyzed phosphotransfer regulates multiple intracellular and extracellular energy-dependent and nucleotide signaling processes, including excitation-contraction coupling, hormone secretion, cell and ciliary motility, nuclear transport, energetics of cell cycle, DNA synthesis and repair, and developmental programming. Metabolomic analyses indicate that cellular, interstitial and blood AMP levels are potential metabolic signals associated with vital functions including body energy sensing, sleep, hibernation and food intake. Either low or excess AMP signaling has been linked to human disease such as diabetes, obesity and hypertrophic cardiomyopathy. Recent studies indicate that derangements in adenylate kinase-mediated energetic signaling due to mutations in AK1, AK2 or AK7 isoforms are associated with hemolytic anemia, reticular dysgenesis and ciliary dyskinesia. Moreover, hormonal, food and antidiabetic drug actions are frequently coupled to alterations of cellular AMP levels and associated signaling. Thus, by monitoring energy state and generating and distributing AMP metabolic signals adenylate kinase represents a unique hub within the cellular homeostatic network.

  8. AMP-activated protein kinase induces actin cytoskeleton reorganization in epithelial cells

    International Nuclear Information System (INIS)

    Miranda, Lisa; Carpentier, Sarah; Platek, Anna; Hussain, Nusrat; Gueuning, Marie-Agnes; Vertommen, Didier; Ozkan, Yurda; Sid, Brice; Hue, Louis; Courtoy, Pierre J.; Rider, Mark H.; Horman, Sandrine

    2010-01-01

    AMP-activated protein kinase (AMPK), a known regulator of cellular and systemic energy balance, is now recognized to control cell division, cell polarity and cell migration, all of which depend on the actin cytoskeleton. Here we report the effects of A769662, a pharmacological activator of AMPK, on cytoskeletal organization and signalling in epithelial Madin-Darby canine kidney (MDCK) cells. We show that AMPK activation induced shortening or radiation of stress fibers, uncoupling from paxillin and predominance of cortical F-actin. In parallel, Rho-kinase downstream targets, namely myosin regulatory light chain and cofilin, were phosphorylated. These effects resembled the morphological changes in MDCK cells exposed to hyperosmotic shock, which led to Ca 2+ -dependent AMPK activation via calmodulin-dependent protein kinase kinase-β(CaMKKβ), a known upstream kinase of AMPK. Indeed, hypertonicity-induced AMPK activation was markedly reduced by the STO-609 CaMKKβ inhibitor, as was the increase in MLC and cofilin phosphorylation. We suggest that AMPK links osmotic stress to the reorganization of the actin cytoskeleton.

  9. The D-SDA Reporting System: Reporting and User Access at DLR-EOC

    OpenAIRE

    Senft, Johanna; Chereji, Cristian; Mario, Winkler; Katrin, Molch; Eberhard, Mikusch

    2015-01-01

    In recent years, Earth observation missions and projects have put ever-increasing emphasis on reporting. Detailed reports are being requested on system performance, user behaviour and processing workload. At the same time reporting is gaining significance for data centres’ internal purposes as well – for identifying bottlenecks and optimizing system performance and reliability. For future planning, Earth Observation data centres increasingly need information-based forecasts. Reliable reports ...

  10. Preliminary systems design study assessment report

    International Nuclear Information System (INIS)

    Mayberry, J.L.; Feizollahi, F.; Del Signore, J.C.

    1991-09-01

    The System Design Study (SDS), part of the Waste Technology Development Department at the Idaho National Engineering Laboratory (INEL), examined techniques available for the remediation of hazardous and transuranic waste stored at the Radioactive Waste Management Complex's Subsurface Disposal Area at the INEL. Using specific technologies, system concepts for treating the buried waste and the surrounding contaminated soil were evaluated. Evaluation included implementability, effectiveness, and cost. The SDS resulted in the development of technology requirements including demonstration, testing, and evaluation activities needed for implementing each concept

  11. Status report on nuclear electric propulsion systems

    Science.gov (United States)

    Stearns, J. W.

    1975-01-01

    Progress in nuclear electric propulsion (NEP) systems for a multipayload multimission vehicle needed in both deep-space missions and a variety of geocentric missions is reviewed. The space system power level is a function of the initial launch vehicle mass, but developments in out-of-core nuclear thermionic direct conversion have broadened design options. Cost, design, and performance parameters are compared for reusable chemical space tugs and NEP reusable space tugs. Improvements in heat pipes, ion engines, and magnetoplasmadynamic arc jet thrust subsystems are discussed.

  12. Frequency of polymorphisms and protein expression of cyclin-dependent kinase inhibitor 1A (CDKN1A in central nervous system tumors

    Directory of Open Access Journals (Sweden)

    Mev Dominguez Valentin

    Full Text Available CONTEXT AND OBJECTIVE: Genetic investigation of central nervous system (CNS tumors provides valuable information about the genes regulating proliferation, differentiation, angiogenesis, migration and apoptosis in the CNS. The aim of our study was to determine the prevalence of genetic polymorphisms (codon 31 and 3' untranslated region, 3'UTR and protein expression of the cyclin-dependent kinase inhibitor 1A (CDKN1A gene in patients with and without CNS tumors. DESIGN AND SETTING: Analytical cross-sectional study with a control group, at the Molecular Biology Laboratory, Pediatric Oncology Department, Hospital das Clínicas de Ribeirão Preto. METHODS: 41 patients with CNS tumors and a control group of 161 subjects without cancer and paires for sex, age and ethnicity were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP. Protein analysis was performed on 36 patients with CNS tumors, using the Western Blotting technique. RESULTS: The frequencies of the heterozygote (Ser/Arg and polymorphic homozygote (Arg/Arg genotypes of codon 31 in the control subjects were 28.0% and 1.2%, respectively. However, the 3'UTR site presented frequencies of 24.2% (C/T and 0.6% (T/T. These frequencies were not statistically different (P > 0.05 from those seen in the patients with CNS tumors (19.4% and 0.0%, codon 31; 15.8% and 2.6%, 3'UTR site. Regarding the protein expression in ependymomas, 66.67% did not express the protein CDKN1A. The results for medulloblastomas and astrocytomas were similar: neither of them expressed the protein (57.14% and 61.54%, respectively. CONCLUSION: No significant differences in protein expression patterns or polymorphisms of CDKN1A in relation to the three types of CNS tumors were observed among Brazilian subjects.

  13. Mer tyrosine kinase promotes the survival of t(1;19)-positive acute lymphoblastic leukemia (ALL) in the central nervous system (CNS).

    Science.gov (United States)

    Krause, Sarah; Pfeiffer, Christian; Strube, Susanne; Alsadeq, Ameera; Fedders, Henning; Vokuhl, Christian; Loges, Sonja; Waizenegger, Jonas; Ben-Batalla, Isabel; Cario, Gunnar; Möricke, Anja; Stanulla, Martin; Schrappe, Martin; Schewe, Denis M

    2015-01-29

    Patients with t(1;19)-positive acute lymphoblastic leukemia (ALL) are prone to central nervous system (CNS) relapses, and expression of the TAM (Tyro3, Axl, and Mer) receptor Mer is upregulated in these leukemias. We examined the functional role of Mer in the CNS in preclinical models and performed correlative studies in 64 t(1;19)-positive and 93 control pediatric ALL patients. ALL cells were analyzed in coculture with human glioma cells and normal rat astrocytes: CNS coculture caused quiescence and protection from methotrexate toxicity in Mer(high) ALL cell lines, which was antagonized by short hairpin RNA-mediated knockdown of Mer. Mer expression was upregulated, prosurvival Akt and mitogen-activated protein kinase signaling were activated, and secretion of the Mer ligand Galectin-3 was stimulated. Mer(high) t(1;19) primary cells caused CNS involvement to a larger extent in murine xenografts than in their Mer(low) counterparts. Leukemic cells from Mer(high) xenografts showed enhanced survival in coculture. Treatment of Mer(high) patient cells with the Mer-specific inhibitor UNC-569 in vivo delayed leukemia onset, reduced CNS infiltration, and prolonged survival of mice. Finally, a correlation between high Mer expression and CNS positivity upon initial diagnosis was observed in t(1;19) patients. Our data provide evidence that Mer is associated with survival in the CNS in t(1;19)-positive ALL, suggesting a role as a diagnostic marker and therapeutic target. © 2015 by The American Society of Hematology.

  14. Mitogen-activated protein kinases mediate Mycobacterium ...

    Indian Academy of Sciences (India)

    2012-01-19

    Jan 19, 2012 ... CD44, an adhesion molecule, has been reported to be a binding site for ... receptors in mediating mitogen-activated protein kinase activation. ... surface expression and tumour necrosis factor-alpha levels, ... Abbreviations used: Abs, antibodies; ANOVA, analysis of variance; AP-1, activator protein -1; BCG, ...

  15. Cedar Avenue driver assist system evaluation report.

    Science.gov (United States)

    2011-12-01

    This paper summarizes an evaluation of the Driver Assist System (DAS) used by the Minnesota Valley Transit Authority (MTVA) for bus shoulder operations. The DAS is a GPS-based technology suite that provides lane-position feedback to the driver via a ...

  16. Cab guidance system - requirements and status report

    Energy Technology Data Exchange (ETDEWEB)

    Zacharias,; Sparmann,

    1984-01-01

    There have been increasing calls from taxi operators for a taxiguidance system based on single, functional modules which can be financed one at a time and thus permit a picemeal extension of their system up to the point of full automation. It is the purpose of this research project to develop such a system in response to these demands. A preliminary investigation clarified the operational and technical prerequisites. On the basis of this study, two firms began, in 1983, to develop, construct and test one aspect of such a system, i.e. the communication between client and headquarters. The results lead us to conclude that our chosen format - a close cooperation between the research consultant, the taxi operators and the firms in charge of development - has been essential for the success of that project. Under the auspices of the research grants for the umbrella project ('Development of Components'), and based on the results on such projects as BON, BFB etc., we have now begun to look into the module 'Wireless Data Transfer' and 'Vehicle Tracing'. A parallel project is investigating the module 'Central Data Processing'.

  17. Laser fusion system design study. Final report

    International Nuclear Information System (INIS)

    1975-01-01

    The following studies were completed: (1) The synthesis of a pointing/control system compatible with existing and advanced laser opto-mechanical configurations. (2) Attainment of the required pointing angle, longitudinal focus, and differential pathlength accuracies. (3) Maximum modularization of the sensor and gimbal assemblies to provide the required accuracies at minimum cost. Detailed information is given on each. (MOW)

  18. Solar thermal repowering systems integration. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Dubberly, L. J.; Gormely, J. E.; McKenzie, A. W.

    1979-08-01

    This report is a solar repowering integration analysis which defines the balance-of-plant characteristics and costs associated with the solar thermal repowering of existing gas/oil-fired electric generating plants. Solar repowering interface requirements for water/steam and salt or sodium-cooled central receivers are defined for unit sizes ranging from 50 MWe non-reheat to 350 MWe reheat. Finally balance-of-plant cost estimates are presented for each of six combinations of plant type, receiver type and percent solar repowering.

  19. Mechanism of polyphosphate kinase from Propionibacterium shermanii

    International Nuclear Information System (INIS)

    Robinson, N.A.

    1986-01-01

    Polyphosphate kinase, which catalyzes the reaction shown below, is one of two enzymes which have been reported to catalyze the synthesis of polyphosphate. Purification performed by ammonium sulfate precipitation (0-40% fraction) was followed by chromatography. The enzyme represents 70% of the protein in the hydroxylapatite pool and is stable at this level of purity. The subunit molecular weight was determined by SDS polyacrylamide gel analysis, (83,000 +/- 3000), nondenaturing polyacrylamide gel electrophoresis, (80,000 and 86,000 daltons), gel filtration (Biogel A 0.5m column was 85,000 +/- 4000.) Polyphosphate kinase appears to be a monomeric enzyme of ∼83,000 daltons. Four assays were developed for polyphosphate kinase. Basic proteins such as polylysine stimulate the synthesis of polyphosphate, these proteins cause precipitation of polyphosphate kinase from relatively impure enzyme extracts: Synthesized polyphosphate interacts noncovalently with the basic protein-enzyme precipitate. Efficient synthesis of polyphosphate requires the addition of either phosphate or short chain polyphosphate. Synthesis did occur at 1/10 the rate when neither of these two compounds were included. Initiation, elongation, and termination events of polyphosphate synthesis were examined. Short chain polyphosphate acts as a primer, with [ 32 P] short-chain polyphosphate incorporation into long chain polyphosphate by the kinase

  20. Janus kinase inhibitors: jackpot or potluck?

    Directory of Open Access Journals (Sweden)

    Pavithran Keechilat

    2012-06-01

    Full Text Available The reports of a unique mutation in the Janus kinase-2 gene (JAK2 in polycythemia vera by several independent groups in 2005 quickly spurred the development of the Janus kinase inhibitors. In one of the great victories of translational research in recent times, the first smallmolecule Janus kinase inhibitor ruxolitinib entered a phase I trial in 2007. With the approval of ruxolitinib by the US Federal Drug Administration in November 2011 for high-risk and intermediate-2 risk myelofibrosis, a change in paradigm has occurred in the management of a subset of myeloproliferative neoplasms (MPN: primary myelofibrosis, post-polycythemia vera myelofibrosis, and post-essential thrombocythemia myelofibrosis. Whereas the current evidence for ruxolitinib only covers high-risk and intermediate-2 risk myelofibrosis, inhibitors with greater potency are likely to offer better disease control and survival advantage in patients belonging to these categories, and possibly to the low-risk and intermediate-1 risk categories of MPN as well. But use of the Janus kinase inhibitors also probably has certain disadvantages, such as toxicity, resistance, withdrawal phenomenon, non-reversal of histology, and an implausible goal of disease clone eradication, some of which could offset the gains. In spite of this, Janus kinase inhibitors are here to stay, and for use in more than just myeloproliferative neoplasms.

  1. Exceptional disfavor for proline at the P + 1 position among AGC and CAMK kinases establishes reciprocal specificity between them and the proline-directed kinases.

    Science.gov (United States)

    Zhu, Guozhi; Fujii, Koichi; Belkina, Natalya; Liu, Yin; James, Michael; Herrero, Juan; Shaw, Stephen

    2005-03-18

    To precisely regulate critical signaling pathways, two kinases that phosphorylate distinct sites on the same protein substrate must have mutually exclusive specificity. Evolution could assure this by designing families of kinase such as basophilic kinases and proline-directed kinase with distinct peptide specificity; their reciprocal peptide specificity would have to be very complete, since recruitment of substrate allows phosphorylation of even rather poor phosphorylation sites in a protein. Here we report a powerful evolutionary strategy that assures distinct substrates for basophilic kinases (PKA, PKG and PKC (AGC) and calmodulin-dependent protein kinase (CAMK)) and proline-directed kinase, namely by the presence or absence of proline at the P + 1 position in substrates. Analysis of degenerate and non-degenerate peptides by in vitro kinase assays reveals that proline at the P + 1 position in substrates functions as a "veto" residue in substrate recognition by AGC and CAMK kinases. Furthermore, analysis of reported substrates of two typical basophilic kinases, protein kinase C and protein kinase A, shows the lowest occurrence of proline at the P + 1 position. Analysis of crystal structures and sequence conservation provides a molecular basis for this disfavor and illustrate its generality.

  2. New Brunswick System Operator 2005 annual report

    International Nuclear Information System (INIS)

    2005-01-01

    New Brunswick's electricity generating mix includes thermal, hydro and nuclear power. Facts about New Brunswick's electricity market were presented with reference to transmission lines; number of interconnecting operators; interconnection capacity; installed generation capacity; generation capacity mix; peak demand for 2004-2005; total energy transactions over a 6 month period and value of electricity over that 6 month period. The independently governed New Brunswick System Operator (NBSO) began operations in 2004 with a mandate to introduce competitive integrated electricity supply to municipal utilities and large industrial customers in order to ensure a reliable and adequate supply of electricity to the Maritime area. The NBSO's first task to launch the transition from monopoly supply to a competitive market is in the beginning stages, but progress has been made in terms of market rules, new computerized systems, and consultations with the Market Advisory Committee who review potential changes to the market rules and tariffs. tabs., figs

  3. LANL environmental restoration site ranking system: System description. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Merkhofer, L.; Kann, A.; Voth, M. [Applied Decision Analysis, Inc., Menlo Park, CA (United States)

    1992-10-13

    The basic structure of the LANL Environmental Restoration (ER) Site Ranking System and its use are described in this document. A related document, Instructions for Generating Inputs for the LANL ER Site Ranking System, contains detailed descriptions of the methods by which necessary inputs for the system will be generated. LANL has long recognized the need to provide a consistent basis for comparing the risks and other adverse consequences associated with the various waste problems at the Lab. The LANL ER Site Ranking System is being developed to help address this need. The specific purpose of the system is to help improve, defend, and explain prioritization decisions at the Potential Release Site (PRS) and Operable Unit (OU) level. The precise relationship of the Site Ranking System to the planning and overall budget processes is yet to be determined, as the system is still evolving. Generally speaking, the Site Ranking System will be used as a decision aid. That is, the system will be used to aid in the planning and budgetary decision-making process. It will never be used alone to make decisions. Like all models, the system can provide only a partial and approximate accounting of the factors important to budget and planning decisions. Decision makers at LANL will have to consider factors outside of the formal system when making final choices. Some of these other factors are regulatory requirements, DOE policy, and public concern. The main value of the site ranking system, therefore, is not the precise numbers it generates, but rather the general insights it provides.

  4. LANL environmental restoration site ranking system: System description. Final report

    International Nuclear Information System (INIS)

    Merkhofer, L.; Kann, A.; Voth, M.

    1992-01-01

    The basic structure of the LANL Environmental Restoration (ER) Site Ranking System and its use are described in this document. A related document, Instructions for Generating Inputs for the LANL ER Site Ranking System, contains detailed descriptions of the methods by which necessary inputs for the system will be generated. LANL has long recognized the need to provide a consistent basis for comparing the risks and other adverse consequences associated with the various waste problems at the Lab. The LANL ER Site Ranking System is being developed to help address this need. The specific purpose of the system is to help improve, defend, and explain prioritization decisions at the Potential Release Site (PRS) and Operable Unit (OU) level. The precise relationship of the Site Ranking System to the planning and overall budget processes is yet to be determined, as the system is still evolving. Generally speaking, the Site Ranking System will be used as a decision aid. That is, the system will be used to aid in the planning and budgetary decision-making process. It will never be used alone to make decisions. Like all models, the system can provide only a partial and approximate accounting of the factors important to budget and planning decisions. Decision makers at LANL will have to consider factors outside of the formal system when making final choices. Some of these other factors are regulatory requirements, DOE policy, and public concern. The main value of the site ranking system, therefore, is not the precise numbers it generates, but rather the general insights it provides

  5. Bifidobacterial recombinant thymidine kinase-ganciclovir gene therapy system induces FasL and TNFR2 mediated antitumor apoptosis in solid tumors

    International Nuclear Information System (INIS)

    Wang, Changdong; Ma, Yongping; Hu, Qiongwen; Xie, Tingting; Wu, Jiayan; Zeng, Fan; Song, Fangzhou

    2016-01-01

    Directly targeting therapeutic suicide gene to a solid tumor is a hopeful approach for cancer gene therapy. Treatment of a solid tumor by an effective vector for a suicide gene remains a challenge. Given the lack of effective treatments, we constructed a bifidobacterial recombinant thymidine kinase (BF-rTK) -ganciclovir (GCV) targeting system (BKV) to meet this requirement and to explore antitumor mechanisms. Bifidobacterium (BF) or BF-rTK was injected intratumorally with or without ganciclovir in a human colo320 intestinal xenograft tumor model. The tumor tissues were analyzed using apoptosis antibody arrays, real time PCR and western blot. The colo320 cell was analyzed by the gene silencing method. Autophagy and necroptosis were also detected in colo320 cell. Meanwhile, three human digestive system xenograft tumor models (colorectal cancer colo320, gastric cancer MKN-45 and liver cancer SSMC-7721) and a breast cancer (MDA-MB-231) model were employed to validate the universality of BF-rTK + GCV in solid tumor gene therapy. The survival rate was evaluated in three human cancer models after the BF-rTK + GCV intratumor treatment. The analysis of inflammatory markers (TNF-α) in tumor indicated that BF-rTK + GCV significantly inhibited TNF-α expression. The results suggested that BF-rTK + GCV induced tumor apoptosis without autophagy and necroptosis occurrence. The apoptosis was transduced by multiple signaling pathways mediated by FasL and TNFR2 and mainly activated the mitochondrial control of apoptosis via Bid and Bim, which was rescued by silencing Bid or/and Bim. However, BF + GCV only induced apoptosis via Fas/FasL signal pathway accompanied with increased P53 expression. We further found that BF-rTK + GCV inhibited the expression of the inflammatory maker of TNF-α. However, BF-rTK + GCV did not result in necroptosis and autophagy. BF-rTK + GCV induced tumor apoptosis mediated by FasL and TNFR2 through the mitochondrial control of apoptosis via Bid and Bim

  6. Tank waste remediation system mission analysis report

    International Nuclear Information System (INIS)

    Acree, C.D.

    1998-01-01

    This document describes and analyzes the technical requirements that the Tank Waste Remediation System (TWRS) must satisfy for the mission. This document further defines the technical requirements that TWRS must satisfy to supply feed to the private contractors' facilities and to store or dispose the immobilized waste following processing in these facilities. This document uses a two phased approach to the analysis to reflect the two-phased nature of the mission

  7. Stereo Viewing System. Innovative Technology Summary Report

    International Nuclear Information System (INIS)

    None

    2000-01-01

    The Stereo Viewing System provides stereoscopic viewing of Light Duty Utility Arm activities. Stereoscopic viewing allows operators to see the depth of objects. This capability improves the control of the Light Duty Utility Arm performed in DOE's underground radioactive waste storage tanks and allows operators to evaluate the depth of pits, seams, and other anomalies. Potential applications include Light Duty Utility Arm deployment operations at the Oak Ridge Reservation, Hanford Site, and the Idaho National Engineering and Environmental Laboratory

  8. Progress report: 1996 Radiation Safety Systems Division

    International Nuclear Information System (INIS)

    Bhagwat, A.M.; Sharma, D.N.; Abani, M.C.; Mehta, S.K.

    1997-01-01

    The activities of Radiation Safety Systems Division include (i) development of specialised monitoring systems and radiation safety information network, (ii) radiation hazards control at the nuclear fuel cycle facilities, the radioisotope programmes at Bhabha Atomic Research Centre (BARC) and for the accelerators programme at BARC and Centre for Advanced Technology (CAT), Indore. The systems on which development and upgradation work was carried out during the year included aerial gamma spectrometer, automated environment monitor using railway network, radioisotope package monitor and air monitors for tritium and alpha active aerosols. Other R and D efforts at the division included assessment of risk for radiation exposures and evaluation of ICRP 60 recommendations in the Indian context, shielding evaluation and dosimetry for the new upcoming accelerator facilities and solid state nuclear track detector techniques for neutron measurements. The expertise of the divisional members was provided for 36 safety committees of BARC and Atomic Energy Regulatory Board (AERB). Twenty three publications were brought out during the year 1996. (author)

  9. Progress report, 1 Jan - 31 Dec 1989. Information Systems Group

    International Nuclear Information System (INIS)

    Loevborg, L.

    1990-04-01

    The report describes the work of the Information Systems Group at Risoe National Laboratory during 1989. The activities may be classified as research into human work and cognition, decision support systems, and process control and process simulation. The report includes a list of staff members. (author)

  10. New Automated System Available for Reporting Safety Concerns | Poster

    Science.gov (United States)

    A new system has been developed for reporting safety issues in the workplace. The Environment, Health, and Safety’s (EHS’) Safety Inspection and Issue Management System (SIIMS) is an online resource where any employee can report a problem or issue, said Siobhan Tierney, program manager at EHS.

  11. FunSETL–Functional Reporting for ERP Systems

    DEFF Research Database (Denmark)

    Nissen, Michael Nebel; Larsen, Ken Friis

    2008-01-01

    One of the essential features of enterprise resource planning systems is the ability to provide the users and decision makers with reports on how the enterprise is running, and to enable the enterprise to provide the authorities the required legal reports.  By their nature these reports needs to ...

  12. A novel colonoscopy reporting system enabling quality assurance

    NARCIS (Netherlands)

    van Doorn, Sascha C.; van Vliet, Joost; Fockens, Paul; Dekker, Evelien

    2014-01-01

    The quality of colonoscopy can only be measured if colonoscopy reports include all key quality indicators. In daily practice, reporting is often incomplete and not standardized. This study describes a novel, structured colonoscopy reporting system, which aims to generate standardized and complete

  13. Systems biology approach identifies the kinase Csnk1a1 as a regulator of the DNA damage response in embryonic stem cells

    DEFF Research Database (Denmark)

    Carreras Puigvert, Jordi; von Stechow, Louise; Siddappa, Ramakrishnaiah

    2013-01-01

    screen targeting all kinases, phosphatases, and transcription factors with global transcriptomics and phosphoproteomics to map the DDR in mouse embryonic stem cells treated with the DNA cross-linker cisplatin. Networks derived from canonical pathways shared in all three data sets were implicated in DNA...

  14. Waste package performance allocation system study report

    International Nuclear Information System (INIS)

    Memory, R.D.

    1994-01-01

    The Waste Package Performance Allocation system study was performed in order to provide a technical basis for the selection of the waste package period of substantially complete containment and its resultant contribution to the overall total system performance. This study began with a reference case based on the current Mined Geologic Disposal System (MGDS) baseline design and added a number of alternative designs. The waste package designs were selected from the designs being considered in detail during Advanced Conceptual Design (ACD). The waste packages considered were multi-barrier packages with a 0.95 cm Alloy 825 inner barrier and a 10, 20, or 45 cm thick carbon steel outer barrier. The waste package capacities varied from 6 to 12 to 21 Pressurized Water Reactor (PWR) fuel assemblies. The vertical borehole and in-drift emplacement modes were also considered, as were thermal loadings of 25, 57, and 114 kW/acre. The repository cost analysis indicated that the 21 PWR in-drift emplacement mode option with the 10 cm and 20 cm outer barrier thicknesses are the least expensive and that the 12 PWR in-drift case has approximately the same cost as the 6 PWR vertical borehole. It was also found that the cost increase from the 10 cm outer barrier waste package to the 20 cm waste package was less per centimeter than the increase from the 20 cm outer barrier waste package to the 45 cm outer barrier waste package. However, the repository cost was nearly linear with the outer barrier thickness for the 21 PWR in-drift case. Finally, corrosion rate estimates are provided and the relationship of repository cost versus waste package lifetime is discussed as is cumulative radionuclide release from the waste package and to the accessible environment for time periods of 10,000 years and 100,000 years

  15. W-026, acceptance test report manipulator system

    International Nuclear Information System (INIS)

    Watson, T.L.

    1997-01-01

    The purpose of the WRAP Manipulator System Acceptance Test Plan (ATP) is to verify that the 4 glovebox sets of WRAP manipulator components, including rail/carriage, slave arm, master controller and auxiliary equipment, meets the requirements of the functional segments of 14590 specification. The demonstration of performance elements of the ATP are performed as a part of the Assembly specifications. Manipulator integration is integrated in the performance testing of the gloveboxes. Each requirement of the Assembly specification will be carried out in conjunction with glovebox performance tests

  16. Final Report of Strongly Interacting Fermion Systems

    International Nuclear Information System (INIS)

    Wilkins, J. W.

    2001-01-01

    There has been significant progress in three broad areas: (A) Optical properties, (B) Large-scale computations, and (C) Many-body systems. In this summary the emphasis is primarily on those papers that point to the research plans. At the same time, some important analytic work is not neglected, some of it even appearing in the description of large-scale Computations. Indeed one of the aims of such computations is to give new insights which lead to development of models capable of simple analytic or nearly analytic analysis

  17. COMWIN Antenna System Fiscal Year 2000 Report

    National Research Council Canada - National Science Library

    Adams, R

    2000-01-01

    .... The Joint Tactical Radio (JTR) requires this frequency. The figure of merit to determine whether the radio is efficient in the band is a Standing Wave Ratio (VSWR) of less than 3:1. The COMWIN antenna system would consist of three antennas. The first antenna, in the form of a vest, would operate in the 30- to 500-MHz band. The helmet antenna would operate in the 500- to 2000 MHz band. An antenna that runs down the edges would operate in the 2- to 30-MHz band.

  18. An automatic drawing system for a report radioactive contamination check

    International Nuclear Information System (INIS)

    Saneyoshi, Keiji; Tomita, Satoru; Yoda, Isao

    2002-01-01

    An Automatic drawing system for a report of surface contamination check in a radiation controlled area has been developed. The system can print out the report applied for the format provided by the law from the raw data that is the output from measuring instruments. The task of a worker is only to insert an FD storing the data into a PC and to push a button. The system also yields contamination maps to indicate contamination points clearly. With this system the time to complete the report from the raw data could be decreased from more than two hours to 4 minutes. (author)

  19. Overview of DOE Radiation Exposure Information Reporting System (REIRS)

    International Nuclear Information System (INIS)

    Fix, J.J.; Briscoe, G.J.; Selby, J.M.; Vallario, E.J.

    1981-05-01

    The purpose of the study is to determine the adequacy of the present system, identify any necessary short-term improvements and propose feasible alternatives for an improved system. The study includes topical reports as follows: current Personnel Dosimetry Practices at DOE Facilities; overview of DOE Radiation Exposure Information Reporting System (REIRS); and alternatives to Provide Upgraded Occupational Exposure Record System. This study constitutes the second report and was a joint effort between Battelle Northwest and EG and G, Idaho Falls. EG and G has been responsible for the respository since the fall of 1978

  20. Innovative technology summary report: Transportable vitrification system

    International Nuclear Information System (INIS)

    1998-09-01

    At the end of the cold war, many of the Department of Energy's (DOE's) major nuclear weapons facilities refocused their efforts on finding technically sound, economic, regulatory compliant, and stakeholder acceptable treatment solutions for the legacy of mixed wastes they had produced. In particular, an advanced stabilization process that could effectively treat the large volumes of settling pond and treatment sludges was needed. Based on this need, DOE and its contractors initiated in 1993 the EM-50 sponsored development effort required to produce a deployable mixed waste vitrification system. As a consequence, the Transportable Vitrification System (TVS) effort was undertaken with the primary requirement to develop and demonstrate the technology and associated facility to effectively vitrify, for compliant disposal, the applicable mixed waste sludges and solids across the various DOE complex sites. After 4 years of development testing with both crucible and pilot-scale melters, the TVS facility was constructed by Envitco, evaluated and demonstrated with surrogates, and then successfully transported to the ORNL ETTP site and demonstrated with actual mixed wastes in the fall of 1997. This paper describes the technology, its performance, the technology applicability and alternatives, cost, regulatory and policy issues, and lessons learned

  1. Systemic mastocytosis: Case report with literature review

    Directory of Open Access Journals (Sweden)

    Mitrović Mirjana

    2012-01-01

    Full Text Available Introduction. Mastocytosis is a clonal neoplastic disorder of the mast cells. The clinical signs and symptoms of mastocytosis are heterogeneous ranging from indolent disease with a longterm survival to a highly aggressive neoplasm with survival of about 6 months. Systemic mastocytosis (SM is characterized by mastocyte infiltration of one or more organs, with or without skin involvment. Case Outline. The presented patient presents a highly challenging diagnostic and therapeutic case. A 46-year-old man was referred to our Centre due to the 7-year-long history of hepatosplenomegaly and mild thrombocytopenia. Ultrasound examination showed hepatosplenomegaly (liver 170 mm; spleen 200 mm, platelet count was 90Č109/L, serum tryptase level was elevated and bone marrow biopsy showed infiltration with mast cells (CD117, CD25 and mast cell tryptase positive. Our patient was diagnosed with aggressive systemic mastocytosis (SM according to WHO Classification (2008, although the clinical course of the disease was indolent, without complications for more than 7 years. Because of the ‘intermediate’ course, this patient was referred to as smouldering or intermediate SM and was not treated with cytostatics. Conclusion. Utilizing the established criteria, indolent SM can be discriminated from the aggressive subvariants of SM in most cases. However, a small group of patients, like our case belongs to the „grey zone“. Therapeutic approach to these patients is individual and prognosis is uncertain.

  2. GO evaluation of a PWR spray system. Final report

    International Nuclear Information System (INIS)

    Long, W.T.

    1975-08-01

    GO is a reliability analysis methodology developed over the years from 1960 to the present by Kaman Sciences Corporation, Colorado Springs, Colorado. In this report the GO methodology is presented and its application demonstrated by performing a reliability analysis of a conceptual PWR Containment Spray System. Certain numerical results obtained are compared with those of a prior fault tree analysis of the same system as documented in the 11 January 1973 draft report, A Fault Tree Evaluation of a PWR Spray System

  3. Molecular Imaging of the ATM Kinase Activity

    Energy Technology Data Exchange (ETDEWEB)

    Williams, Terence M. [Department of Radiation Oncology, Ohio State University, Columbus, Ohio (United States); Nyati, Shyam [Department of Radiation Oncology, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Center for Molecular Imaging, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Ross, Brian D. [Department of Radiation Oncology, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Department of Radiology, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Rehemtulla, Alnawaz, E-mail: alnawaz@umich.edu [Department of Radiation Oncology, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Center for Molecular Imaging, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Department of Radiology, University of Michigan Medical Center, Ann Arbor, Michigan (United States)

    2013-08-01

    Purpose: Ataxia telangiectasia mutated (ATM) is a serine/threonine kinase critical to the cellular DNA-damage response, including from DNA double-strand breaks. ATM activation results in the initiation of a complex cascade of events including DNA damage repair, cell cycle checkpoint control, and survival. We sought to create a bioluminescent reporter that dynamically and noninvasively measures ATM kinase activity in living cells and subjects. Methods and Materials: Using the split luciferase technology, we constructed a hybrid cDNA, ATM-reporter (ATMR), coding for a protein that quantitatively reports on changes in ATM kinase activity through changes in bioluminescence. Results: Treatment of ATMR-expressing cells with ATM inhibitors resulted in a dose-dependent increase in bioluminescence activity. In contrast, induction of ATM kinase activity upon irradiation resulted in a decrease in reporter activity that correlated with ATM and Chk2 activation by immunoblotting in a time-dependent fashion. Nuclear targeting improved ATMR sensitivity to both ATM inhibitors and radiation, whereas a mutant ATMR (lacking the target phosphorylation site) displayed a muted response. Treatment with ATM inhibitors and small interfering (si)RNA-targeted knockdown of ATM confirm the specificity of the reporter. Using reporter expressing xenografted tumors demonstrated the ability of ATMR to report in ATM activity in mouse models that correlated in a time-dependent fashion with changes in Chk2 activity. Conclusions: We describe the development and validation of a novel, specific, noninvasive bioluminescent reporter that enables monitoring of ATM activity in real time, in vitro and in vivo. Potential applications of this reporter include the identification and development of novel ATM inhibitors or ATM-interacting partners through high-throughput screens and in vivo pharmacokinetic/pharmacodynamic studies of ATM inhibitors in preclinical models.

  4. Joint Logistics Systems Center Reporting of Systems Development Costs

    National Research Council Canada - National Science Library

    1998-01-01

    ...." The Joint Logistics Systems Center (JLSC) was organized in FY 1992 to accomplish Corporate Information Management goals for the depot maintenance and supply management business areas of the DoD Working Capital Funds...

  5. Application examples of the reports of the NEA Incident Reporting System of the OECD and evolution of the system

    International Nuclear Information System (INIS)

    Libmann, J.

    1989-06-01

    Some reports of the work group no. 1 of the Nuclear Installations Security Committee of NEA, are summarized. An example of the report coding system concerning human factors, is given. The aim of the study is to improve the report contents as well as the coding system. In this case, a fast data selection is possible, and allows an efficient analysis of a particular situation. Moreover, the corrective procedures of the nuclear installation conception or operation can be easily modified, by the national organisations. Due to the improvements in quality, the opinion of the member countries on the incident reporting systems efficiency was enhanced [fr

  6. Tyrosine kinases in rheumatoid arthritis

    Directory of Open Access Journals (Sweden)

    Kobayashi Akiko

    2011-08-01

    Full Text Available Abstract Rheumatoid arthritis (RA is an inflammatory, polyarticular joint disease. A number of cellular responses are involved in the pathogenesis of rheumatoid arthritis, including activation of inflammatory cells and cytokine expression. The cellular responses involved in each of these processes depends on the specific signaling pathways that are activated; many of which include protein tyrosine kinases. These pathways include the mitogen-activated protein kinase pathway, Janus kinases/signal transducers and activators transcription pathway, spleen tyrosine kinase signaling, and the nuclear factor κ-light-chain-enhancer of activated B cells pathway. Many drugs are in development to target tyrosine kinases for the treatment of RA. Based on the number of recently published studies, this manuscript reviews the role of tyrosine kinases in the pathogenesis of RA and the potential role of kinase inhibitors as new therapeutic strategies of RA.

  7. Condenser inleakage monitoring system development. Final report

    International Nuclear Information System (INIS)

    Kassen, W.R.; Putkey, T.A.; Sawochka, S.G.; Pearl, W.L.; Clouse, M.E.

    1982-09-01

    An instrument/hardware package for air and condenser cooling water inleakage location employing the helium and freon techniques was designed and fabricated. The package consists of design details for tracer gas distribution hardware, injection plenums, and a sample preconditioner and instrument module. Design of the package was based on an evaluation of helium and freon leak detectors and a survey of utility user's experience with the helium and freon techniques. The applicability of the instrument/hardware package to air and cooling water inleakage location was demonstrated at Pacific Gas and Electric Company's Moss Landing Station. The use of calibrated leaks indicated that cooling water leaks down to 1.5 x 10 -4 gpm (0.56 ml/min) and air leaks down to 0.05 cfm were readily detectable with the helium technique, whereas a 4 x 10 -4 gpm (1.5 ml/min) liquid leak was the readily detectable minimum via the freon technique. The field demonstration and in-house detector testing showed the helium technique to be preferable to the freon technique for inleakage location at PWRs, BWRs, and fossil-fueled systems

  8. Second-generation inhibitors of Bruton tyrosine kinase

    Directory of Open Access Journals (Sweden)

    Jingjing Wu

    2016-09-01

    Full Text Available Abstract Bruton tyrosine kinase (BTK is a critical effector molecule for B cell development and plays a major role in lymphoma genesis. Ibrutinib is the first-generation BTK inhibitor. Ibrutinib has off-target effects on EGFR, ITK, and Tec family kinases, which explains the untoward effects of ibrutinib. Resistance to ibrutinib was also reported. The C481S mutation in the BTK kinase domain was reported to be a major mechanism of resistance to ibrutinib. This review summarizes the clinical development of novel BTK inhibitors, ACP-196 (acalabrutinib, ONO/GS-4059, and BGB-3111.

  9. Fluorescence Polarization Screening Assays for Small Molecule Allosteric Modulators of ABL Kinase Function.

    Directory of Open Access Journals (Sweden)

    Prerna Grover

    Full Text Available The ABL protein-tyrosine kinase regulates intracellular signaling pathways controlling diverse cellular processes and contributes to several forms of cancer. The kinase activity of ABL is repressed by intramolecular interactions involving its regulatory Ncap, SH3 and SH2 domains. Small molecules that allosterically regulate ABL kinase activity through its non-catalytic domains may represent selective probes of ABL function. Here we report a screening assay for chemical modulators of ABL kinase activity that target the regulatory interaction of the SH3 domain with the SH2-kinase linker. This fluorescence polarization (FP assay is based on a purified recombinant ABL protein consisting of the N-cap, SH3 and SH2 domains plus the SH2-kinase linker (N32L protein and a short fluorescein-labeled probe peptide that binds to the SH3 domain. In assay development experiments, we found that the probe peptide binds to the recombinant ABL N32L protein in vitro, producing a robust FP signal that can be competed with an excess of unlabeled peptide. The FP signal is not observed with control N32L proteins bearing either an inactivating mutation in the SH3 domain or enhanced SH3:linker interaction. A pilot screen of 1200 FDA-approved drugs identified four compounds that specifically reduced the FP signal by at least three standard deviations from the untreated controls. Secondary assays showed that one of these hit compounds, the antithrombotic drug dipyridamole, enhances ABL kinase activity in vitro to a greater extent than the previously described ABL agonist, DPH. Docking studies predicted that this compound binds to a pocket formed at the interface of the SH3 domain and the linker, suggesting that it activates ABL by disrupting this regulatory interaction. These results show that screening assays based on the non-catalytic domains of ABL can identify allosteric small molecule regulators of kinase function, providing a new approach to selective drug discovery

  10. Electronic clinical safety reporting system: a benefits evaluation.

    Science.gov (United States)

    Elliott, Pamela; Martin, Desmond; Neville, Doreen

    2014-06-11

    Eastern Health, a large health care organization in Newfoundland and Labrador (NL), started a staged implementation of an electronic occurrence reporting system (used interchangeably with "clinical safety reporting system") in 2008, completing Phase One in 2009. The electronic clinical safety reporting system (CSRS) was designed to replace a paper-based system. The CSRS involves reporting on occurrences such as falls, safety/security issues, medication errors, treatment and procedural mishaps, medical equipment malfunctions, and close calls. The electronic system was purchased from a vendor in the United Kingdom that had implemented the system in the United Kingdom and other places, such as British Columbia. The main objective of the new system was to improve the reporting process with the goal of improving clinical safety. The project was funded jointly by Eastern Health and Canada Health Infoway. The objectives of the evaluation were to: (1) assess the CSRS on achieving its stated objectives (particularly, the benefits realized and lessons learned), and (2) identify contributions, if any, that can be made to the emerging field of electronic clinical safety reporting. The evaluation involved mixed methods, including extensive stakeholder participation, pre/post comparative study design, and triangulation of data where possible. The data were collected from several sources, such as project documentation, occurrence reporting records, stakeholder workshops, surveys, focus groups, and key informant interviews. The findings provided evidence that frontline staff and managers support the CSRS, identifying both benefits and areas for improvement. Many benefits were realized, such as increases in the number of occurrences reported, in occurrences reported within 48 hours, in occurrences reported by staff other than registered nurses, in close calls reported, and improved timelines for notification. There was also user satisfaction with the tool regarding ease of use

  11. 75 FR 25137 - Changes to Standard Numbering System, Vessel Identification System, and Boating Accident Report...

    Science.gov (United States)

    2010-05-07

    ...-2003-14963] RIN 1625-AB45 Changes to Standard Numbering System, Vessel Identification System, and... System (SNS), the Vessel Identification System (VIS), and casualty reporting; require validation of... Standard Numbering System U.S.C. United States Code VIS Vessel Identification System III. Background Coast...

  12. Waste retrieval sluicing system data acquisition system acceptance test report

    International Nuclear Information System (INIS)

    Bevins, R.R.

    1998-01-01

    This document describes the test procedure for the Project W-320 Tank C-106 Sluicing Data Acquisition System (W-320 DAS). The Software Test portion will test items identified in the WRSS DAS System Description (SD), HNF-2115. Traceability to HNF-2115 will be via a reference that follows in parenthesis, after the test section title. The Field Test portion will test sensor operability, analog to digital conversion, and alarm setpoints for field instrumentation. The W-320 DAS supplies data to assist thermal modeling of tanks 241-C-106 and 241-AY-102. It is designed to be a central repository for information from sources that would otherwise have to be read, recorded, and integrated manually. Thus, completion of the DAS requires communication with several different data collection devices and output to a usable PC data formats. This test procedure will demonstrate that the DAS functions as required by the project requirements stated in Section 3 of the W-320 DAS System Description, HNF-2115

  13. Federal Automated Information System of Nuclear Material Control and Accounting: Uniform System of Reporting Documents

    International Nuclear Information System (INIS)

    Pitel, M V; Kasumova, L; Babcock, R A; Heinberg, C

    2003-01-01

    One of the fundamental regulations of the Russian State System for Nuclear Material Accounting and Control (SSAC), ''Basic Nuclear Material Control and Accounting Rules,'' directed that a uniform report system be developed to support the operation of the SSAC. According to the ''Regulation on State Nuclear Material Control and Accounting,'' adopted by the Russian Federation Government, Minatom of Russia is response for the development and adoption of report forms, as well as the reporting procedure and schedule. The report forms are being developed in tandem with the creation of an automated national nuclear material control and accounting system, the Federal Information System (FIS). The forms are in different stages of development and implementation. The first report forms (the Summarized Inventory Listing (SIL), Summarized Inventory Change Report (SICR) and federal and agency registers of nuclear material) have already been created and implemented. The second set of reports (nuclear material movement reports and the special anomaly report) is currently in development. A third set of reports (reports on import/export operations, and foreign nuclear material temporarily located in the Russian Federation) is still in the conceptual stage. To facilitate the development of a unified document system, the FIS must establish a uniform philosophy for the reporting system and determine the requirements for each reporting level, adhering to the following principles: completeness--the unified report system provides the entire range of information that the FIS requires to perform SSAC tasks; requisite level of detail; hierarchical structure--each report is based on the information provided in a lower-level report and is the source of information for reports at the next highest level; consistency checking--reports can be checked against other reports. A similar philosophy should eliminate redundancy in the different reports, support a uniform approach to the contents of

  14. Status Report on Power System Transformation: A 21st Century Power Partnership Report

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Mackay [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Martinot, Eric [Beijing Inst. of Technology (China); Cox, Sadie [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Speer, Bethany [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Zinaman, Owen [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Booth, Sam [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Zissler, Romain [Japan Renewable Energy Foundation (Japan); Cochran, Jaquelin [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Soonee, S. K. [Power System Operation Corporation, Ltd (India); Audinet, Pierre [World Bank Energy Sector Management Assistance Program, Washington, DC (United States); Munuera, Luis [International Energy Agency, Paris (France); Arent, Doug [Joint Inst. for Strategic Energy Analysis, Washington, DC (United States)

    2015-05-27

    This report has three primary goals: (1) to articulate the concept of power system transformation; (2) to explore the current global landscape of ‘innovations’ that constitute power system transformation and provide evidence of how these innovations are emerging; and (3) to suggest an analytical framework for assessing the status of power system transformation on an on-going basis.

  15. Regulation of Autophagy by Kinases

    International Nuclear Information System (INIS)

    Sridharan, Savitha; Jain, Kirti; Basu, Alakananda

    2011-01-01

    Autophagy is a process of self-degradation that maintains cellular viability during periods of metabolic stress. Although autophagy is considered a survival mechanism when faced with cellular stress, extensive autophagy can also lead to cell death. Aberrations in autophagy are associated with several diseases, including cancer. Therapeutic exploitation of this process requires a clear understanding of its regulation. Although the core molecular components involved in the execution of autophagy are well studied there is limited information on how cellular signaling pathways, particularly kinases, regulate this complex process. Protein kinases are integral to the autophagy process. Atg1, the first autophagy-related protein identified, is a serine/threonine kinase and it is regulated by another serine/threonine kinase mTOR. Emerging studies suggest the participation of many different kinases in regulating various components/steps of this catabolic process. This review focuses on the regulation of autophagy by several kinases with particular emphasis on serine/threonine protein kinases such as mTOR, AMP-activated protein kinase, Akt, mitogen-activated protein kinase (ERK, p38 and JNK) and protein kinase C that are often deregulated in cancer and are important therapeutic targets

  16. Regulation of Autophagy by Kinases

    Energy Technology Data Exchange (ETDEWEB)

    Sridharan, Savitha; Jain, Kirti; Basu, Alakananda, E-mail: alakananda.basu@unthsc.edu [Department of Molecular Biology and Immunology, Institute for Cancer Research, University of North Texas Health Science Center, Fort Worth, TX 76107 (United States)

    2011-06-09

    Autophagy is a process of self-degradation that maintains cellular viability during periods of metabolic stress. Although autophagy is considered a survival mechanism when faced with cellular stress, extensive autophagy can also lead to cell death. Aberrations in autophagy are associated with several diseases, including cancer. Therapeutic exploitation of this process requires a clear understanding of its regulation. Although the core molecular components involved in the execution of autophagy are well studied there is limited information on how cellular signaling pathways, particularly kinases, regulate this complex process. Protein kinases are integral to the autophagy process. Atg1, the first autophagy-related protein identified, is a serine/threonine kinase and it is regulated by another serine/threonine kinase mTOR. Emerging studies suggest the participation of many different kinases in regulating various components/steps of this catabolic process. This review focuses on the regulation of autophagy by several kinases with particular emphasis on serine/threonine protein kinases such as mTOR, AMP-activated protein kinase, Akt, mitogen-activated protein kinase (ERK, p38 and JNK) and protein kinase C that are often deregulated in cancer and are important therapeutic targets.

  17. Regulation of Autophagy by Kinases

    Science.gov (United States)

    Sridharan, Savitha; Jain, Kirti; Basu, Alakananda

    2011-01-01

    Autophagy is a process of self-degradation that maintains cellular viability during periods of metabolic stress. Although autophagy is considered a survival mechanism when faced with cellular stress, extensive autophagy can also lead to cell death. Aberrations in autophagy are associated with several diseases, including cancer. Therapeutic exploitation of this process requires a clear understanding of its regulation. Although the core molecular components involved in the execution of autophagy are well studied there is limited information on how cellular signaling pathways, particularly kinases, regulate this complex process. Protein kinases are integral to the autophagy process. Atg1, the first autophagy-related protein identified, is a serine/threonine kinase and it is regulated by another serine/threonine kinase mTOR. Emerging studies suggest the participation of many different kinases in regulating various components/steps of this catabolic process. This review focuses on the regulation of autophagy by several kinases with particular emphasis on serine/threonine protein kinases such as mTOR, AMP-activated protein kinase, Akt, mitogen-activated protein kinase (ERK, p38 and JNK) and protein kinase C that are often deregulated in cancer and are important therapeutic targets. PMID:24212825

  18. Regulation of Autophagy by Kinases

    Directory of Open Access Journals (Sweden)

    Savitha Sridharan

    2011-06-01

    Full Text Available Autophagy is a process of self-degradation that maintains cellular viability during periods of metabolic stress. Although autophagy is considered a survival mechanism when faced with cellular stress, extensive autophagy can also lead to cell death. Aberrations in autophagy are associated with several diseases, including cancer. Therapeutic exploitation of this process requires a clear understanding of its regulation. Although the core molecular components involved in the execution of autophagy are well studied there is limited information on how cellular signaling pathways, particularly kinases, regulate this complex process. Protein kinases are integral to the autophagy process. Atg1, the first autophagy-related protein identified, is a serine/threonine kinase and it is regulated by another serine/threonine kinase mTOR. Emerging studies suggest the participation of many different kinases in regulating various components/steps of this catabolic process. This review focuses on the regulation of autophagy by several kinases with particular emphasis on serine/threonine protein kinases such as mTOR, AMP-activated kinase, Akt, mitogen-activated protein kinase (ERK, p38 and JNK and protein kinase C that are often deregulated in cancer and are important therapeutic targets.

  19. Identification and Characterization of Amlexanox as a G Protein-Coupled Receptor Kinase 5 Inhibitor

    Directory of Open Access Journals (Sweden)

    Kristoff T. Homan

    2014-10-01

    Full Text Available G protein-coupled receptor kinases (GRKs have been implicated in human diseases ranging from heart failure to diabetes. Previous studies have identified several compounds that selectively inhibit GRK2, such as paroxetine and balanol. Far fewer selective inhibitors have been reported for GRK5, a target for the treatment of cardiac hypertrophy, and the mechanism of action of reported compounds is unknown. To identify novel scaffolds that selectively inhibit GRK5, a differential scanning fluorometry screen was used to probe a library of 4480 compounds. The best hit was amlexanox, an FDA-approved anti-inflammatory, anti-allergic immunomodulator. The crystal structure of amlexanox in complex with GRK1 demonstrates that its tricyclic aromatic ring system forms ATP-like interactions with the hinge of the kinase domain, which is likely similar to how this drug binds to IκB kinase ε (IKKε, another kinase known to be inhibited by this compound. Amlexanox was also able to inhibit myocyte enhancer factor 2 transcriptional activity in neonatal rat ventricular myocytes in a manner consistent with GRK5 inhibition. The GRK1 amlexanox structure thus serves as a springboard for the rational design of inhibitors with improved potency and selectivity for GRK5 and IKKε.

  20. TYROSINE KINASE INHIBITORS AND PREGNANCY

    Directory of Open Access Journals (Sweden)

    Elisabetta Abruzzese

    2014-04-01

    Full Text Available The management of patients with chronic myeloid leukemia (CML during pregnancy has became recently a matter of continuous debate.  The introduction of the Tyrosine Kinase Inhibitors (TKIs in clinical practice has dramatically changed the prognosis of CML patients.  Patients diagnosed in chronic phase can reasonably expect many years of excellent disease control and good quality of life, as well as a normal life expectancy.  This fact has come the necessity to address issues relating to fertility and pregnancy. Physicians are not infrequently being asked for advice regarding the need for, and or the appropriateness of, stopping treatment in order to conceive. In this report we will review the data published in terms of fertility, conception, pregnancy, pregnancy outcome and illness control for all the approved TKIs, as well as suggest how to manage a planned and/or unplanned pregnancy.

  1. Report: Improvements Needed in Key EPA Information System Security Practices

    Science.gov (United States)

    Report #10-P-0146, June 15, 2010. Williams Adley found that EPA program offices lacked evidence that they planned and executed tests of information system security controls as required by federal requirements.

  2. Recommendation Report: EJournals/EBooks A-Z Management System

    KAUST Repository

    Ramli, Rindra M.

    2014-01-01

    This is a recommendation report for KAUST Library on the Ejournals / EBooks AZ Management systems project. It briefly described the issues faced by the ERM Team, project plan overview and the project findings as well as the recommendation(s).

  3. SUBSURFACE VOLATIZATION AND VENTILATION SYSTEM (SVVS) - INNOVATIVE TECHNOLOGY REPORT

    Science.gov (United States)

    This report summarizes the findings associated with a Demonstration Test of Environmental Improvement Technologies’ (EIT) Subsurface Volatilization and Ventilation System (SVVS) process. The technology was evaluated under the EPA Superfund Innovative Technology Evaluation (SITE) ...

  4. Status report on the ADVANCED LIGHT SOURCE control system

    International Nuclear Information System (INIS)

    Magyary, S.; Chin, M.; Fahmie, M.; Lancaster, H.; Molinari, P.; Robb, A.; Timossi, C.; Young, J.

    1992-01-01

    This paper is a status report on the ADVANCED LIGHT SOURCE (ALS) control system. The current status, performance data, and future plans will be discussed. Manpower, scheduling, and costs issues are addressed. (author)

  5. Progressive Systemic sclerosis, manifested like malabsorption syndrome. Case report

    International Nuclear Information System (INIS)

    Ortiz Piza, Gabriel Jaime; Gonzalez Vasquez, Carlos Mario

    2005-01-01

    We report the case of a 32 year old woman whose first manifestation of systemic sclerosis was malabsorption syndrome. The small bowel series was the clue to the diagnosis, confirmed by laboratory tests and progression of the disease

  6. Intelligent transportation systems business plan for Kentucky : final report.

    Science.gov (United States)

    2001-09-01

    This report presents a Business Plan for Intelligent Transportation Systems (ITS) in Kentucky. The purpose of the Business Plan is to define ITS projects that are planned for implementation from 2002 through 2007. The list of projects contained withi...

  7. Implementing agreement on photovoltaic power systems - Annual report 2006

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    This annual report for the International Energy Agency (IEA) reports on the programme's activities in 2006. The IEA Photovoltaic Power Systems Programme (PVPS) is one of the collaborative research and development agreements established within the IEA. Its mission is to enhance international collaboration efforts which support the development and deployment of photovoltaic solar energy. In this annual report, the programme's mission and its strategies for reaching four objectives are reviewed and status reports on the programme's various tasks and sub-tasks are presented. The tasks include the exchange and dissemination of information on photovoltaic power systems, the performance, reliability and analysis of photovoltaic systems, a study on very large scale photovoltaic power generation system, photovoltaic services for developing countries, urban-scale PV applications and hybrid systems within mini-grids. The status and prospects in the 21 countries and organisations participating in the programme are presented. Along with country-specific topics, industry activities, research, development and demonstration projects, applications, education and governmental activities as well as future activities are reviewed. Finally, completed tasks are reviewed. These include the use of photovoltaic power systems in stand-alone and island applications, grid interconnection of building integrated and other distributed photovoltaic power systems, design and operation of modular photovoltaic plants for large scale power generation and photovoltaic power systems in the built environment. The report is completed with a list of Executive Committee members and Operating Agents.

  8. Implementing agreement on photovoltaic power systems - Annual report 2007

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2008-07-01

    This annual report for the International Energy Agency (IEA) reports on the programme's activities in 2007. The IEA Photovoltaic Power Systems Programme (PVPS) is one of the collaborative research and development agreements established within the IEA. Its mission is to enhance international collaboration efforts which support the development and deployment of photovoltaic solar energy. In this annual report, the programme's mission and its strategies for reaching four objectives are reviewed and status reports on the programme's various tasks and sub-tasks are presented. The tasks include the exchange and dissemination of information on photovoltaic power systems, the performance, reliability and analysis of photovoltaic systems, a study on very large scale photovoltaic power generation system, photovoltaic services for developing countries, urban-scale PV applications, hybrid systems within mini-grids and PV environmental health and safety activities. The status and prospects in the 22 countries and organisations participating in the programme are presented. Along with country-specific topics, industry activities, research, development and demonstration projects, applications, education and governmental activities as well as future activities are reviewed. Finally, completed tasks are reviewed. These include the use of photovoltaic power systems in stand-alone and island applications, grid interconnection of building integrated and other distributed photovoltaic power systems, design and operation of modular photovoltaic plants for large scale power generation and photovoltaic power systems in the built environment. The report is completed with a list of Executive Committee members and Operating Agents.

  9. Implementing agreement on photovoltaic power systems - Annual report 2008

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2009-07-01

    This annual report for the International Energy Agency (IEA) reports on the programme's activities in 2008. The IEA Photovoltaic Power Systems Programme (PVPS) is one of the collaborative research and development agreements established within the IEA. Its mission is to enhance international collaboration efforts which support the development and deployment of photovoltaic solar energy. In this annual report, the programme's mission and its strategies for reaching four objectives are reviewed and status reports on the programme's various tasks and sub-tasks are presented. The tasks include the exchange and dissemination of information on photovoltaic power systems, a study on very large scale photovoltaic power generation system, photovoltaic services for developing countries, urban-scale PV applications, hybrid systems within mini-grids as well as health and safety activities. The status and prospects in the 23 countries and organisations participating in the programme are presented. Along with country-specific topics, industry activities, research, development and demonstration projects, applications, education and governmental activities as well as future activities are reviewed. Finally, completed tasks are reviewed. These include the performance, reliability and analysis of photovoltaic systems, the use of photovoltaic power systems in stand-alone and island applications, grid interconnection of building integrated and other distributed photovoltaic power systems, design and operation of modular photovoltaic plants for large scale power generation and photovoltaic power systems in the built environment. The report is completed with a list of Executive Committee members and Operating Agents.

  10. Implementing agreement on photovoltaic power systems - Annual report 2006

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    This annual report for the International Energy Agency (IEA) reports on the programme's activities in 2006. The IEA Photovoltaic Power Systems Programme (PVPS) is one of the collaborative research and development agreements established within the IEA. Its mission is to enhance international collaboration efforts which support the development and deployment of photovoltaic solar energy. In this annual report, the programme's mission and its strategies for reaching four objectives are reviewed and status reports on the programme's various tasks and sub-tasks are presented. The tasks include the exchange and dissemination of information on photovoltaic power systems, the performance, reliability and analysis of photovoltaic systems, a study on very large scale photovoltaic power generation system, photovoltaic services for developing countries, urban-scale PV applications and hybrid systems within mini-grids. The status and prospects in the 21 countries and organisations participating in the programme are presented. Along with country-specific topics, industry activities, research, development and demonstration projects, applications, education and governmental activities as well as future activities are reviewed. Finally, completed tasks are reviewed. These include the use of photovoltaic power systems in stand-alone and island applications, grid interconnection of building integrated and other distributed photovoltaic power systems, design and operation of modular photovoltaic plants for large scale power generation and photovoltaic power systems in the built environment. The report is completed with a list of Executive Committee members and Operating Agents.

  11. Implementing agreement on photovoltaic power systems - Annual report 2007

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2008-07-01

    This annual report for the International Energy Agency (IEA) reports on the programme's activities in 2007. The IEA Photovoltaic Power Systems Programme (PVPS) is one of the collaborative research and development agreements established within the IEA. Its mission is to enhance international collaboration efforts which support the development and deployment of photovoltaic solar energy. In this annual report, the programme's mission and its strategies for reaching four objectives are reviewed and status reports on the programme's various tasks and sub-tasks are presented. The tasks include the exchange and dissemination of information on photovoltaic power systems, the performance, reliability and analysis of photovoltaic systems, a study on very large scale photovoltaic power generation system, photovoltaic services for developing countries, urban-scale PV applications, hybrid systems within mini-grids and PV environmental health and safety activities. The status and prospects in the 22 countries and organisations participating in the programme are presented. Along with country-specific topics, industry activities, research, development and demonstration projects, applications, education and governmental activities as well as future activities are reviewed. Finally, completed tasks are reviewed. These include the use of photovoltaic power systems in stand-alone and island applications, grid interconnection of building integrated and other distributed photovoltaic power systems, design and operation of modular photovoltaic plants for large scale power generation and photovoltaic power systems in the built environment. The report is completed with a list of Executive Committee members and Operating Agents.

  12. Implementing agreement on photovoltaic power systems - Annual report 2008

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2009-07-01

    This annual report for the International Energy Agency (IEA) reports on the programme's activities in 2008. The IEA Photovoltaic Power Systems Programme (PVPS) is one of the collaborative research and development agreements established within the IEA. Its mission is to enhance international collaboration efforts which support the development and deployment of photovoltaic solar energy. In this annual report, the programme's mission and its strategies for reaching four objectives are reviewed and status reports on the programme's various tasks and sub-tasks are presented. The tasks include the exchange and dissemination of information on photovoltaic power systems, a study on very large scale photovoltaic power generation system, photovoltaic services for developing countries, urban-scale PV applications, hybrid systems within mini-grids as well as health and safety activities. The status and prospects in the 23 countries and organisations participating in the programme are presented. Along with country-specific topics, industry activities, research, development and demonstration projects, applications, education and governmental activities as well as future activities are reviewed. Finally, completed tasks are reviewed. These include the performance, reliability and analysis of photovoltaic systems, the use of photovoltaic power systems in stand-alone and island applications, grid interconnection of building integrated and other distributed photovoltaic power systems, design and operation of modular photovoltaic plants for large scale power generation and photovoltaic power systems in the built environment. The report is completed with a list of Executive Committee members and Operating Agents.

  13. Engineered Barrier System performance requirements systems study report. Revision 02

    Energy Technology Data Exchange (ETDEWEB)

    Balady, M.A.

    1997-01-14

    This study evaluates the current design concept for the Engineered Barrier System (EBS), in concert with the current understanding of the geologic setting to assess whether enhancements to the required performance of the EBS are necessary. The performance assessment calculations are performed by coupling the EBS with the geologic setting based on the models (some of which were updated for this study) and assumptions used for the 1995 Total System Performance Assessment (TSPA). The need for enhancements is determined by comparing the performance assessment results against the EBS related performance requirements. Subsystem quantitative performance requirements related to the EBS include the requirement to allow no more than 1% of the waste packages (WPs) to fail before 1,000 years after permanent closure of the repository, as well as a requirement to control the release rate of radionuclides from the EBS. The EBS performance enhancements considered included additional engineered components as well as evaluating additional performance available from existing design features but for which no performance credit is currently being taken.

  14. Engineered Barrier System performance requirements systems study report. Revision 02

    International Nuclear Information System (INIS)

    Balady, M.A.

    1997-01-01

    This study evaluates the current design concept for the Engineered Barrier System (EBS), in concert with the current understanding of the geologic setting to assess whether enhancements to the required performance of the EBS are necessary. The performance assessment calculations are performed by coupling the EBS with the geologic setting based on the models (some of which were updated for this study) and assumptions used for the 1995 Total System Performance Assessment (TSPA). The need for enhancements is determined by comparing the performance assessment results against the EBS related performance requirements. Subsystem quantitative performance requirements related to the EBS include the requirement to allow no more than 1% of the waste packages (WPs) to fail before 1,000 years after permanent closure of the repository, as well as a requirement to control the release rate of radionuclides from the EBS. The EBS performance enhancements considered included additional engineered components as well as evaluating additional performance available from existing design features but for which no performance credit is currently being taken

  15. Kinome profiling of Arabidopsis using arrays of kinase consensus substrates

    Directory of Open Access Journals (Sweden)

    Pieterse Corné MJ

    2007-02-01

    Full Text Available Abstract Background Kinome profiling aims at the parallel analysis of kinase activities in a cell. Novel developed arrays containing consensus substrates for kinases are used to assess those kinase activities. The arrays described in this paper were already used to determine kinase activities in mammalian systems, but since substrates from many organisms are present we decided to test these arrays for the determination of kinase activities in the model plant species Arabidopsis thaliana. Results Kinome profiling using Arabidopsis cell extracts resulted in the labelling of many consensus peptides by kinases from the plant, indicating the usefulness of this kinome profiling tool for plants. Method development showed that fresh and frozen plant material could be used to make cell lysates containing active kinases. Dilution of the plant extract increased the signal to noise ratio and non-radioactive ATP enhances full development of spot intensities. Upon infection of Arabidopsis with an avirulent strain of the bacterial pathogen Pseudomonas syringae pv. tomato, we could detect differential kinase activities by measuring phosphorylation of consensus peptides. Conclusion We show that kinome profiling on arrays with consensus substrates can be used to monitor kinase activities in plants. In a case study we show that upon infection with avirulent P. syringae differential kinase activities can be found. The PepChip can for example be used to purify (unknown kinases that play a role in P. syringae infection. This paper shows that kinome profiling using arrays of consensus peptides is a valuable new tool to study signal-transduction in plants. It complements the available methods for genomics and proteomics research.

  16. Java-based PACS and reporting system for nuclear medicine

    Science.gov (United States)

    Slomka, Piotr J.; Elliott, Edward; Driedger, Albert A.

    2000-05-01

    In medical imaging practice, images and reports often need be reviewed and edited from many locations. We have designed and implemented a Java-based Remote Viewing and Reporting System (JaRRViS) for a nuclear medicine department, which is deployed as a web service, at the fraction of the cost dedicated PACS systems. The system can be extended to other imaging modalities. JaRRViS interfaces to the clinical patient databases of imaging workstations. Specialized nuclear medicine applets support interactive displays of data such as 3-D gated SPECT with all the necessary options such as cine, filtering, dynamic lookup tables, and reorientation. The reporting module is implemented as a separate applet using Java Foundation Classes (JFC) Swing Editor Kit and allows composition of multimedia reports after selection and annotation of appropriate images. The reports are stored on the server in the HTML format. JaRRViS uses Java Servlets for the preparation and storage of final reports. The http links to the reports or to the patient's raw images with applets can be obtained from JaRRViS by any Hospital Information System (HIS) via standard queries. Such links can be sent via e-mail or included as text fields in any HIS database, providing direct access to the patient reports and images via standard web browsers.

  17. 2014-15 System Accountability Report. Appendix: Data Tables

    Science.gov (United States)

    Board of Governors, State University System of Florida, 2016

    2016-01-01

    This system accountability report provides the Data Tables that are appended to the 2014-2015 accountability report. The data tables include detailed statistical information presented in a tabular format on the following subject matter: (1) Financial Resources; (2) Personnel; (3) Enrollment; (4) Undergraduate Education; (5) Graduate Education; and…

  18. Report on the Audit of Unattended Ground Sensor Systems

    Science.gov (United States)

    1991-02-26

    This final report on the Audit of Unattended Ground Sensor Systems is for your information and use. Comments on the draft were considered in...preparing the final report and changes have been made where appropriate. We performed the audit from February through August 1990. The objective was to

  19. Consumer response to a report card comparing healthcare systems.

    Science.gov (United States)

    Braun, Barbara L; Kind, Elizabeth A; Fowles, Jinnet B; Suarez, Walter G

    2002-06-01

    Report cards to date have focused on quality of care in health plans rather than within healthcare delivery systems. The purpose of this study was to evaluate consumer response to the first healthcare system-level report card. Qualitative assessment of consumer response. We conducted 5 focus groups of community members to evaluate consumer response to the report card; 2 included community club members, 3 included community-dwelling retired persons. Discussions were audiotaped and transcribed; comments were categorized by topic area from the script, and common themes identified. Focus group participants, in general, were unaware of the current emphasis on medical quality improvement initiatives. However, they believed that the opinion that the descriptive clinic information and patient survey data contained in the report card would be most useful mainly for choosing a healthcare system if they were dissatisfied with current medical care, if their healthcare options changed, or if they were in poor health. Personal experience was considered a more trustworthy measure of healthcare quality than were patient survey results. Trustworthiness was perceived to be higher if the report card sponsor was not affiliated with the healthcare systems being evaluated. Participants also believed care system administrators should use the data to enact positive clinic-level and physician-level changes. Healthcare consumers appreciated the attention to patient experiences and supported healthcare quality improvement initiatives. Report cards were considered important for choosing a healthcare system in certain circumstances and for guiding quality improvement efforts at all levels.

  20. Implementing agreement on photovoltaic power systems - Annual report 2000

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-07-01

    This annual report for the International Energy Agency (IEA) reports on the programme's activities in 2000. The IEA Photovoltaic Power Systems Programme (PVPS) is one of the collaborative research and development agreements established within the IEA. Its mission is to enhance international collaboration efforts which support the development and deployment of photovoltaic solar energy. In this annual report, the programme's mission and its strategies for reaching four objectives are reviewed. Status reports on the programme's various tasks and sub-tasks are presented. The tasks include the exchange and dissemination of information on photovoltaic power systems, the operational performance and design of photovoltaic systems, the use of photovoltaic power systems in stand-alone and island applications, the grid interconnection of building-integrated and other distributed photovoltaic power systems, photovoltaic power systems in the built environment, very large scale photovoltaic power generation systems and the deployment of photovoltaic technologies in developing countries. The status and prospects in the 20 countries participating in the programme are presented. Along with country-specific topics, industry activities, research, development and demonstration projects, applications, education and governmental activities as well as future activities are reviewed. The report is completed with a list of Executive Committee members and Operating Agents.

  1. Implementing agreement on photovoltaic power systems - Annual report 2004

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2005-07-01

    This annual report for the International Energy Agency (IEA) reports on the programme's activities in 2004. The IEA Photovoltaic Power Systems Programme (PVPS) is one of the collaborative research and development agreements established within the IEA. Its mission is to enhance international collaboration efforts which support the development and deployment of photovoltaic solar energy. In this annual report, the programme's mission and its strategies for reaching four objectives are reviewed and status reports on the programme's various tasks and sub-tasks are presented. The tasks include the exchange and dissemination of information on photovoltaic power systems, the performance, reliability and analysis of photovoltaic systems, the use of photovoltaic power systems in stand-alone and island applications, a study on very large scale photovoltaic power generation system, photovoltaic services for developing countries and urban-scale PV applications. The status and prospects in the 21 countries and organisations participating in the programme are presented. Along with country-specific topics, industry activities, research, development and demonstration projects, applications, education and governmental activities as well as future activities are reviewed. Finally, completed tasks are reviewed. These include grid interconnection of building integrated and other distributed photovoltaic power systems, design and operation of modular photovoltaic plants for large scale power generation and photovoltaic power systems in the built environment. The report is completed with a list of Executive Committee members and Operating Agents.

  2. Implementing agreement on photovoltaic power systems - Annual report 2004

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2005-07-01

    This annual report for the International Energy Agency (IEA) reports on the programme's activities in 2004. The IEA Photovoltaic Power Systems Programme (PVPS) is one of the collaborative research and development agreements established within the IEA. Its mission is to enhance international collaboration efforts which support the development and deployment of photovoltaic solar energy. In this annual report, the programme's mission and its strategies for reaching four objectives are reviewed and status reports on the programme's various tasks and sub-tasks are presented. The tasks include the exchange and dissemination of information on photovoltaic power systems, the performance, reliability and analysis of photovoltaic systems, the use of photovoltaic power systems in stand-alone and island applications, a study on very large scale photovoltaic power generation system, photovoltaic services for developing countries and urban-scale PV applications. The status and prospects in the 21 countries and organisations participating in the programme are presented. Along with country-specific topics, industry activities, research, development and demonstration projects, applications, education and governmental activities as well as future activities are reviewed. Finally, completed tasks are reviewed. These include grid interconnection of building integrated and other distributed photovoltaic power systems, design and operation of modular photovoltaic plants for large scale power generation and photovoltaic power systems in the built environment. The report is completed with a list of Executive Committee members and Operating Agents.

  3. Implementing agreement on photovoltaic power systems - Annual report 2001

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-07-01

    This annual report for the International Energy Agency (IEA) reports on the programme's activities in 2001. The IEA Photovoltaic Power Systems Programme (PVPS) is one of the collaborative research and development agreements established within the IEA. Its mission is to enhance international collaboration efforts which support the development and deployment of photovoltaic solar energy. In this annual report, the programme's mission and its strategies for reaching four objectives are reviewed. Status reports on the programme's various tasks and sub-tasks are presented. The tasks include the exchange and dissemination of information on photovoltaic power systems, the operational performance, maintenance and sizing of photovoltaic systems, the use of photovoltaic power systems in stand-alone and island applications, grid interconnection of building integrated and other distributed photovoltaic power systems, photovoltaic power systems in the built environment, a study on very large scale photovoltaic power generation system and the deployment of photovoltaic technologies in developing countries. The status and prospects in the 20 countries participating in the programme are presented. Along with country-specific topics, industry activities, research, development and demonstration projects, applications, education and governmental activities as well as future activities are reviewed. The report is completed with a list of Executive Committee members and Operating Agents.

  4. Implementing agreement on photovoltaic power systems - Annual report 2003

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-07-01

    This annual report for the International Energy Agency (IEA) reports on the programme's activities in 2003. The IEA Photovoltaic Power Systems Programme (PVPS) is one of the collaborative research and development agreements established within the IEA. Its mission is to enhance international collaboration efforts which support the development and deployment of photovoltaic solar energy. In this annual report, the programme's mission and its strategies for reaching four objectives are reviewed. The programme's tenth anniversary is noted. Status reports on the programme's various tasks and sub-tasks are presented. The tasks include the exchange and dissemination of information on photovoltaic power systems, the operational performance, maintenance and sizing of photovoltaic systems, the use of photovoltaic power systems in stand-alone and island applications, grid interconnection of building integrated and other distributed photovoltaic power systems, photovoltaic power systems in the built environment, a study on very large scale photovoltaic power generation system, the deployment of photovoltaic technologies in developing countries and urban-scale PV applications. The status and prospects in the 20 countries and organisations participating in the programme are presented. Along with country-specific topics, industry activities, research, development and demonstration projects, applications, education and governmental activities as well as future activities are reviewed. The report is completed with a list of Executive Committee members and Operating Agents.

  5. Implementing agreement on photovoltaic power systems - Annual report 2005

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-07-01

    This annual report for the International Energy Agency (IEA) reports on the programme's activities in 2005. The IEA Photovoltaic Power Systems Programme (PVPS) is one of the collaborative research and development agreements established within the IEA. Its mission is to enhance international collaboration efforts which support the development and deployment of photovoltaic solar energy. In this annual report, the programme's mission and its strategies for reaching four objectives are reviewed and status reports on the programme's various tasks and sub-tasks are presented. The tasks include the exchange and dissemination of information on photovoltaic power systems, the performance, reliability and analysis of photovoltaic systems, a study on very large scale photovoltaic power generation system, photovoltaic services for developing countries and urban-scale PV applications. The status and prospects in the 21 countries and organisations participating in the programme are presented. Along with country-specific topics, industry activities, research, development and demonstration projects, applications, education and governmental activities as well as future activities are reviewed. Finally, completed tasks are reviewed. These include the use of photovoltaic power systems in stand-alone and island applications, grid interconnection of building integrated and other distributed photovoltaic power systems, design and operation of modular photovoltaic plants for large scale power generation and photovoltaic power systems in the built environment. The report is completed with a list of Executive Committee members and Operating Agents.

  6. Implementing agreement on photovoltaic power systems - Annual report 2005

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-07-01

    This annual report for the International Energy Agency (IEA) reports on the programme's activities in 2005. The IEA Photovoltaic Power Systems Programme (PVPS) is one of the collaborative research and development agreements established within the IEA. Its mission is to enhance international collaboration efforts which support the development and deployment of photovoltaic solar energy. In this annual report, the programme's mission and its strategies for reaching four objectives are reviewed and status reports on the programme's various tasks and sub-tasks are presented. The tasks include the exchange and dissemination of information on photovoltaic power systems, the performance, reliability and analysis of photovoltaic systems, a study on very large scale photovoltaic power generation system, photovoltaic services for developing countries and urban-scale PV applications. The status and prospects in the 21 countries and organisations participating in the programme are presented. Along with country-specific topics, industry activities, research, development and demonstration projects, applications, education and governmental activities as well as future activities are reviewed. Finally, completed tasks are reviewed. These include the use of photovoltaic power systems in stand-alone and island applications, grid interconnection of building integrated and other distributed photovoltaic power systems, design and operation of modular photovoltaic plants for large scale power generation and photovoltaic power systems in the built environment. The report is completed with a list of Executive Committee members and Operating Agents.

  7. Nuclear Power Safety Reporting System. Final evaluation results

    International Nuclear Information System (INIS)

    Finlayson, F.C.; Newton, R.D.

    1986-02-01

    This document presents the results of a study conducted by the US Nuclear Regulatory Commission of an unobtrusive, voluntary, anonymous third-party managed, nonpunitive human factors data gathering system (the Nuclear power Safety Reporting System - NPSRS) for the nuclear electric power production industry. The data to be gathered by the NPSRS are intended for use in identifying and quantifying the factors that contribute to the occurrence of significant safety incidents involving humans in nuclear power plants. The NPSRS has been designed to encourage participation in the System through guarantees of reporter anonymity provided by a third-party organization that would be responsible for NPSRS management. As additional motivation to reporters for contributing data to the NPSRS, conditional waivers of NRC disciplinary action would be provided to individuals. These conditional waivers of immunity would apply to potential violations of NRC regulations that might be disclosed through reports submitted to the System about inadvertent, noncriminal incidents in nuclear plants. This document summarizes the overall results of the study of the NPSRS concept. In it, a functional description of the NPSRS is presented together with a review and assessment of potential problem areas that might be met if the System were implemented. Conclusions and recommendations resulting from the study are also presented. A companion volume (NUREG/CR-4133, Nuclear Power Safety Reporting System: Implementation and Operational Specifications'') presented in detail the elements, requirements, forms, and procedures for implementing and operating the System. 13 refs

  8. Bacterial Protein-Tyrosine Kinases

    DEFF Research Database (Denmark)

    Shi, Lei; Kobir, Ahasanul; Jers, Carsten

    2010-01-01

    in exopolysaccharide production, virulence, DNA metabolism, stress response and other key functions of the bacterial cell. BY-kinases act through autophosphorylation (mainly in exopolysaccharide production) and phosphorylation of other proteins, which have in most cases been shown to be activated by tyrosine......Bacteria and Eukarya share essentially the same family of protein-serine/threonine kinases, also known as the Hanks-type kinases. However, when it comes to protein-tyrosine phosphorylation, bacteria seem to have gone their own way. Bacterial protein-tyrosine kinases (BY-kinases) are bacterial...... and highlighted their importance in bacterial physiology. Having no orthologues in Eukarya, BY-kinases are receiving a growing attention from the biomedical field, since they represent a particularly promising target for anti-bacterial drug design....

  9. The kinase activity of the Ser/Thr kinase BUB1 promotes TGF-β signaling.

    Science.gov (United States)

    Nyati, Shyam; Schinske-Sebolt, Katrina; Pitchiaya, Sethuramasundaram; Chekhovskiy, Katerina; Chator, Areeb; Chaudhry, Nauman; Dosch, Joseph; Van Dort, Marcian E; Varambally, Sooryanarayana; Kumar-Sinha, Chandan; Nyati, Mukesh Kumar; Ray, Dipankar; Walter, Nils G; Yu, Hongtao; Ross, Brian Dale; Rehemtulla, Alnawaz

    2015-01-06

    Transforming growth factor-β (TGF-β) signaling regulates cell proliferation and differentiation, which contributes to development and disease. Upon binding TGF-β, the type I receptor (TGFBRI) binds TGFBRII, leading to the activation of the transcription factors SMAD2 and SMAD3. Using an RNA interference screen of the human kinome and a live-cell reporter for TGFBR activity, we identified the kinase BUB1 (budding uninhibited by benzimidazoles-1) as a key mediator of TGF-β signaling. BUB1 interacted with TGFBRI in the presence of TGF-β and promoted the heterodimerization of TGFBRI and TGFBRII. Additionally, BUB1 interacted with TGFBRII, suggesting the formation of a ternary complex. Knocking down BUB1 prevented the recruitment of SMAD3 to the receptor complex, the phosphorylation of SMAD2 and SMAD3 and their interaction with SMAD4, SMAD-dependent transcription, and TGF-β-mediated changes in cellular phenotype including epithelial-mesenchymal transition (EMT), migration, and invasion. Knockdown of BUB1 also impaired noncanonical TGF-β signaling mediated by the kinases AKT and p38 MAPK (mitogen-activated protein kinase). The ability of BUB1 to promote TGF-β signaling depended on the kinase activity of BUB1. A small-molecule inhibitor of the kinase activity of BUB1 (2OH-BNPP1) and a kinase-deficient mutant of BUB1 suppressed TGF-β signaling and formation of the ternary complex in various normal and cancer cell lines. 2OH-BNPP1 administration to mice bearing lung carcinoma xenografts reduced the amount of phosphorylated SMAD2 in tumor tissue. These findings indicated that BUB1 functions as a kinase in the TGF-β pathway in a role beyond its established function in cell cycle regulation and chromosome cohesion. Copyright © 2015, American Association for the Advancement of Science.

  10. Cocoa Procyanidins Suppress Transformation by Inhibiting Mitogen-activated Protein Kinase Kinase*S⃞

    Science.gov (United States)

    Kang, Nam Joo; Lee, Ki Won; Lee, Dong Eun; Rogozin, Evgeny A.; Bode, Ann M.; Lee, Hyong Joo; Dong, Zigang

    2008-01-01

    Cocoa was shown to inhibit chemically induced carcinogenesis in animals and exert antioxidant activity in humans. However, the molecular mechanisms of the chemopreventive potential of cocoa and its active ingredient(s) remain unknown. Here we report that cocoa procyanidins inhibit neoplastic cell transformation by suppressing the kinase activity of mitogen-activated protein kinase kinase (MEK). A cocoa procyanidin fraction (CPF) and procyanidin B2 at 5 μg/ml and 40 μm, respectively, inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced neoplastic transformation of JB6 P+ mouse epidermal (JB6 P+) cells by 47 and 93%, respectively. The TPA-induced promoter activity and expression of cyclooxygenase-2, which is involved in tumor promotion and inflammation, were dose-dependently inhibited by CPF or procyanidin B2. The activation of activator protein-1 and nuclear factor-κB induced by TPA was also attenuated by CPF or procyanidin B2. The TPA-induced phosphorylation of MEK, extracellular signal-regulated kinase, and p90 ribosomal s6 kinase was suppressed by CPF or procyanidin B2. In vitro and ex vivo kinase assay data demonstrated that CPF or procyanidin B2 inhibited the kinase activity of MEK1 and directly bound with MEK1. CPF or procyanidin B2 suppressed JB6 P+ cell transformation induced by epidermal growth factor or H-Ras, both of which are known to be involved in MEK/ERK signal activation. In contrast, theobromine (up to 80 μm) had no effect on TPA-induced transformation, cyclooxygenase-2 expression, the transactivation of activator protein-1 or nuclear factor-κB, or MEK. Notably, procyanidin B2 exerted stronger inhibitory effects compared with PD098059 (a well known pharmacological inhibitor of MEK) on MEK1 activity and neoplastic cell transformation. PMID:18519570

  11. Protein kinase inhibitor peptide (PKI): a family of endogenous neuropeptides that modulate neuronal cAMP-dependent protein kinase function.

    Science.gov (United States)

    Dalton, George D; Dewey, William L

    2006-02-01

    Signal transduction cascades involving cAMP-dependent protein kinase are highly conserved among a wide variety of organisms. Given the universal nature of this enzyme it is not surprising that cAMP-dependent protein kinase plays a critical role in numerous cellular processes. This is particularly evident in the nervous system where cAMP-dependent protein kinase is involved in neurotransmitter release, gene transcription, and synaptic plasticity. Protein kinase inhibitor peptide (PKI) is an endogenous thermostable peptide that modulates cAMP-dependent protein kinase function. PKI contains two distinct functional domains within its amino acid sequence that allow it to: (1) potently and specifically inhibit the activity of the free catalytic subunit of cAMP-dependent protein kinase and (2) export the free catalytic subunit of cAMP-dependent protein kinase from the nucleus. Three distinct PKI isoforms (PKIalpha, PKIbeta, PKIgamma) have been identified and each isoform is expressed in the brain. PKI modulates neuronal synaptic activity, while PKI also is involved in morphogenesis and symmetrical left-right axis formation. In addition, PKI also plays a role in regulating gene expression induced by cAMP-dependent protein kinase. Future studies should identify novel physiological functions for endogenous PKI both in the nervous system and throughout the body. Most interesting will be the determination whether functional differences exist between individual PKI isoforms which is an intriguing possibility since these isoforms exhibit: (1) cell-type specific tissue expression patterns, (2) different potencies for the inhibition of cAMP-dependent protein kinase activity, and (3) expression patterns that are hormonally, developmentally and cell-cycle regulated. Finally, synthetic peptide analogs of endogenous PKI will continue to be invaluable tools that are used to elucidate the role of cAMP-dependent protein kinase in a variety of cellular processes throughout the nervous

  12. INL Control System Situational Awareness Technology Annual Report 2012

    Energy Technology Data Exchange (ETDEWEB)

    Gordon Rueff; Bryce Wheeler; Todd Vollmer; Tim McJunkin; Robert Erbes

    2012-10-01

    The overall goal of this project is to develop an interoperable set of tools to provide a comprehensive, consistent implementation of cyber security and overall situational awareness of control and sensor network implementations. The operation and interoperability of these tools will fill voids in current technological offerings and address issues that remain an impediment to the security of control systems. This report provides an FY 2012 update on the Sophia, Mesh Mapper, Intelligent Cyber Sensor, and Data Fusion projects with respect to the year-two tasks and annual reporting requirements of the INL Control System Situational Awareness Technology report (July 2010).

  13. FOCUS: a fire management planning system -- final report

    Science.gov (United States)

    Frederick W. Bratten; James B. Davis; George T. Flatman; Jerold W. Keith; Stanley R. Rapp; Theodore G. Storey

    1981-01-01

    FOCUS (Fire Operational Characteristics Using Simulation) is a computer simulation model for evaluating alternative fire management plans. This final report provides a broad overview of the FOCUS system, describes two major modules-fire suppression and cost, explains the role in the system of gaming large fires, and outlines the support programs and ways of...

  14. Operational test report for 2706-T complex liquid transfer system

    International Nuclear Information System (INIS)

    BENZEL, H.R.

    1999-01-01

    This document is the Operational Test Report (OTR). It enters the Record Copy of the W-259 Operational Test Procedure (HNF-3610) into the document retrieval system. Additionally, the OTR summarizes significant issues associated with testing the 2706-T waste liquid transfer and storage system

  15. Standard-E hydrogen monitoring system shop acceptance test report

    International Nuclear Information System (INIS)

    Schneider, T.C.

    1997-01-01

    The purpose of this report is to document that the Standard-E Hydrogen Monitoring Systems (SHMS-E), fabricated by Mid-Columbia Engineering (MCE) for installation on the Waste Tank Farms in the Hanford 200 Areas, are constructed as intended by the design. The ATP performance will verify proper system fabrication

  16. Case Report: Systemic Lupus Erythematosus Presenting as Acute ...

    African Journals Online (AJOL)

    We hereby report a case of a 20 year‑old female who presented to us in an acute hypoadrenal state and was found to have Systemic lupus erythematosus with renal involvement. Patient was successfully managed with steroids and improved clinically. Keywords: Addison's disease, Autoimmune diseases, Systemic lupus ...

  17. Disability Evaluation Systems Analysis and Research Annual Report 2015

    Science.gov (United States)

    2016-03-21

    Army and Air Force had higher percentages of reserve component disability evaluations, likely due to the inclusion of National Guard service members...Annual Report 2015 Disability Evaluation Systems Analysis and Research Prepared by Accession Medical Standards Analysis and Research Activity...Preventive Medicine Branch Walter Reed Army Institute of Research Silver Spring, Maryland Disability Evaluation Systems Analysis and Research

  18. Disability Evaluation System Analysis and Research Annual Report 2015

    Science.gov (United States)

    2016-03-11

    Inclusion of laboratory and diagnostic information on the medical condition or injury that precipitated the disability evaluation in each service’s...Annual Report 2015 Disability Evaluation Systems Analysis and Research Prepared by Accession Medical Standards Analysis and Research Activity...Preventive Medicine Branch Walter Reed Army Institute of Research Silver Spring, Maryland Disability Evaluation Systems Analysis and Research

  19. Satellite Search and Rescue System Studies: Alarm and Position Reporting.

    Science.gov (United States)

    alarm and position reporting ( ALPR ) techniques, and the operational or planned spacecraft which might be available for piggybacking the ALPR payload...Several system concepts were then developed to perform the ALPR functions. The candidates were screened and the preferred system concepts were chosen

  20. Test report - caustic addition system operability test procedure

    International Nuclear Information System (INIS)

    Parazin, R.E.

    1995-01-01

    This Operability Test Report documents the test results of test procedure WHC-SD-WM-OTP-167 ''Caustic Addition System Operability Test Procedure''. The Objective of the test was to verify the operability of the 241-AN-107 Caustic Addition System. The objective of the test was met

  1. 10 CFR 50.73 - Licensee event report system.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Licensee event report system. 50.73 Section 50.73 Energy NUCLEAR REGULATORY COMMISSION DOMESTIC LICENSING OF PRODUCTION AND UTILIZATION FACILITIES Inspections... systems, including: emergency diesel generators (EDGs); hydroelectric facilities used in lieu of EDGs at...

  2. A Novel Reporting System to Improve Accuracy in Appendicitis Imaging

    Science.gov (United States)

    Godwin, Benjamin D.; Drake, Frederick T.; Simianu, Vlad V.; Shriki, Jabi E.; Hippe, Daniel S.; Dighe, Manjiri; Bastawrous, Sarah; Cuevas, Carlos; Flum, David; Bhargava, Puneet

    2015-01-01

    OBJECTIVE The purpose of this study was to ascertain if standardized radiologic reporting for appendicitis imaging increases diagnostic accuracy. MATERIALS AND METHODS We developed a standardized appendicitis reporting system that includes objective imaging findings common in appendicitis and a certainty score ranging from 1 (definitely not appendicitis) through 5 (definitely appendicitis). Four radiologists retrospectively reviewed the preoperative CT scans of 96 appendectomy patients using our reporting system. The presence of appendicitis-specific imaging findings and certainty scores were compared with final pathology. These comparisons were summarized using odds ratios (ORs) and the AUC. RESULTS The appendix was visualized on CT in 89 patients, of whom 71 (80%) had pathologically proven appendicitis. Imaging findings associated with appendicitis included appendiceal diameter (odds ratio [OR] = 14 [> 10 vs appendicitis. In this initially indeterminate group, using the standardized reporting system, radiologists assigned higher certainty scores (4 or 5) in 21 of the 28 patients with appendicitis (75%) and lower scores (1 or 2) in five of the seven patients without appendicitis (71%) (AUC = 0.90; p = 0.001). CONCLUSION Standardized reporting and grading of objective imaging findings correlated well with postoperative pathology and may decrease the number of CT findings reported as indeterminate for appendicitis. Prospective evaluation of this reporting system on a cohort of patients with clinically suspected appendicitis is currently under way. PMID:26001230

  3. Protein kinase substrate identification on functional protein arrays

    Directory of Open Access Journals (Sweden)

    Zhou Fang

    2008-02-01

    Full Text Available Abstract Background Over the last decade, kinases have emerged as attractive therapeutic targets for a number of different diseases, and numerous high throughput screening efforts in the pharmaceutical community are directed towards discovery of compounds that regulate kinase function. The emerging utility of systems biology approaches has necessitated the development of multiplex tools suitable for proteomic-scale experiments to replace lower throughput technologies such as mass spectroscopy for the study of protein phosphorylation. Recently, a new approach for identifying substrates of protein kinases has applied the miniaturized format of functional protein arrays to characterize phosphorylation for thousands of candidate protein substrates in a single experiment. This method involves the addition of protein kinases in solution to arrays of immobilized proteins to identify substrates using highly sensitive radioactive detection and hit identification algorithms. Results To date, the factors required for optimal performance of protein array-based kinase substrate identification have not been described. In the current study, we have carried out a detailed characterization of the protein array-based method for kinase substrate identification, including an examination of the effects of time, buffer compositions, and protein concentration on the results. The protein array approach was compared to standard solution-based assays for assessing substrate phosphorylation, and a correlation of greater than 80% was observed. The results presented here demonstrate how novel substrates for protein kinases can be quickly identified from arrays containing thousands of human proteins to provide new clues to protein kinase function. In addition, a pooling-deconvolution strategy was developed and applied that enhances characterization of specific kinase-substrate relationships and decreases reagent consumption. Conclusion Functional protein microarrays are an

  4. Tyrosine Kinase Gene Expression Profiling in Prostate Cancer

    National Research Council Canada - National Science Library

    Weier, Heinz-Ulrich

    2001-01-01

    ... of these genes parallels the progression of tumors to a more malignant phenotype. We developed a DNA micro-array based screening system to monitor the level of expression of tyrosine kinase (tk...

  5. Tyrosine Kinase Gene Expression Profiling in Prostate Cancer

    National Research Council Canada - National Science Library

    Weier, Heinz-Ulrich

    2002-01-01

    ... of these genes parallels the progression of tumors to a more malignant phenotype. We developed a DNA micro-array based screening system to monitor the level of expression of tyrosine kinase (tk...

  6. Computer-based systems important to safety (COMPSIS) - Reporting guidelines

    International Nuclear Information System (INIS)

    1999-07-01

    The objective of this procedure is to help the user to prepare an COMPSIS report on an event so that important lessons learned are most efficiently transferred to the database. This procedure focuses on the content of the information to be provided in the report rather than on its format. The established procedure follows to large extend the procedure chosen by the IRS incident reporting system. However this database is built for I and C equipment with the purpose of the event report database to collect and disseminate information on events of significance involving Computer-Based Systems important to safety in nuclear power plants, and feedback conclusions and lessons learnt from such events. For events where human performance is dominant to draw lessons, more detailed guidance on the specific information that should be supplied is spelled out in the present procedure. This guidance differs somewhat from that for the provision of technical information, and takes into account that the engineering world is usually less familiar with human behavioural analysis than with technical analysis. The events to be reported to the COMPSIS database should be based on the national reporting criteria in the participating member countries. The aim is that all reports including computer based systems that meet each country reporting criteria should be reported. The database should give a broad picture of events/incidents occurring in operation with computer control systems. As soon as an event has been identified, the insights and lessons learnt to be conveyed to the international nuclear community shall be clearly identified. On the basis of the description of the event, the event shall be analyzed in detail under the aspect of direct and potential impact to plant safety functions. The first part should show the common involvement of operation and safety systems and the second part should show the special aspects of I and C functions, hardware and software

  7. Interfacing system isolation experience review. Final report, August 1991

    International Nuclear Information System (INIS)

    1991-08-01

    A light water reactor power plant has auxiliary systems interconnected with the reactor coolant system that are not designed for reactor operating pressure. These principally include the shutdown heat removal systems and various emergency core cooling injection systems. There are multiple isolation valves that prevent rector vessel pressure from causing overpressurization in low pressure interfacing systems. Combinations of hardware failures or operational errors are necessary to expose these systems to overpressurization. This experience review provides insights regarding the risk that an auxiliary system might become over pressurized from the reactor system. While analyses show that for the pressures involved the probability of auxiliary system failure is low, the auxiliary system conceivably might fail outside of containment while the plant is at power. Such a potential event has come to be called an interfacing system loss of coolant accident (ISLOCA). This report provides a compilation of occurrences where valve leakage, valve failure, or valve mispositioning played a role in the ability to maintain interfacing system isolation. Seventeen U.S. BWR events, twenty three U.S. PWR events and one foreign event are discussed in the report. Eleven of the U.S. BWR events and ten U.S. PWR events are judged to relate directly to the so-called ISLOCA event in that they fulfilled one or more of the failures necessary to cause an ISLOCA. (author)

  8. Laser fusion systems for industrial process heat. Third semiannual report

    International Nuclear Information System (INIS)

    Bates, F.J.; Denning, R.S.; Dykhuizen, R.C.; Goldthwaite, W.H.; Kok, K.D.; Skelton, J.C.

    1979-01-01

    This report concentrates not only on the design of the laser fusion system but also on the cost of this system and the costs of alternative sources of energy that are expected to be in competition with the laser fusion system. The absolute values of the cost of the laser fusion system are limited by the estimates of the cost of the components and subsystems making up the laser fusion energy station. The method used in calculating costs of the laser fusion and alternative systems are laid out in detail

  9. Energy storage systems program report for FY1996

    Energy Technology Data Exchange (ETDEWEB)

    Butler, P.C.

    1997-05-01

    Sandia National Laboratories, New Mexico, conducts the Energy Storage Systems Program, which is sponsored by the US Department of Energy`s Office of Utility Technologies. The goal of this program is to assist industry in developing cost-effective energy storage systems as a resource option by 2000. Sandia is responsible for the engineering analyses, contracted development, and testing of energy storage systems for stationary applications. This report details the technical achievements realized during fiscal year 1996.

  10. Utility battery storage systems program report for FY 94

    Energy Technology Data Exchange (ETDEWEB)

    Butler, P.C.

    1995-03-01

    Sandia National Laboratories, New Mexico, conducts the Utility Battery Storage Systems Program, which is sponsored by the US Department of Energy`s Office of Energy Management. The goal of this program is to assist industry in developing cost-effective battery systems as a utility resource option by 2000. Sandia is responsible for the engineering analyses, contracted development, and testing of rechargeable batteries and systems for utility energy storage applications. This report details the technical achievements realized during fiscal year 1994.

  11. CIKS, a connection to Ikappa B kinase and stress-activated protein kinase.

    Science.gov (United States)

    Leonardi, A; Chariot, A; Claudio, E; Cunningham, K; Siebenlist, U

    2000-09-12

    Pathogens, inflammatory signals, and stress cause acute transcriptional responses in cells. The induced expression of genes in response to these signals invariably involves transcription factors of the NF-kappaB and AP-1/ATF families. Activation of NF-kappaB factors is thought to be mediated primarily via IkappaB kinases (IKK), whereas that of AP-1/ATF can be mediated by stress-activated protein kinases (SAPKs; also named Jun kinases or JNKs). IKKalpha and IKKbeta are two catalytic subunits of a core IKK complex that also contains the regulatory subunit NEMO (NF-kappaB essential modulator)/IKKgamma. The latter protein is essential for activation of the IKKs, but its mechanism of action is not known. Here we describe the molecular cloning of CIKS (connection to IKK and SAPK/JNK), a previously unknown protein that directly interacts with NEMO/IKKgamma in cells. When ectopically expressed, CIKS stimulates IKK and SAPK/JNK kinases and it transactivates an NF-kappaB-dependent reporter. Activation of NF-kappaB is prevented in the presence of kinase-deficient, interfering mutants of the IKKs. CIKS may help to connect upstream signaling events to IKK and SAPK/JNK modules. CIKS could coordinate the activation of two stress-induced signaling pathways, functions reminiscent of those noted for tumor necrosis factor receptor-associated factor adaptor proteins.

  12. CIKS, a connection to IκB kinase and stress-activated protein kinase

    Science.gov (United States)

    Leonardi, Antonio; Chariot, Alain; Claudio, Estefania; Cunningham, Kirk; Siebenlist, Ulrich

    2000-01-01

    Pathogens, inflammatory signals, and stress cause acute transcriptional responses in cells. The induced expression of genes in response to these signals invariably involves transcription factors of the NF-κB and AP-1/ATF families. Activation of NF-κB factors is thought to be mediated primarily via IκB kinases (IKK), whereas that of AP-1/ATF can be mediated by stress-activated protein kinases (SAPKs; also named Jun kinases or JNKs). IKKα and IKKβ are two catalytic subunits of a core IKK complex that also contains the regulatory subunit NEMO (NF-κB essential modulator)/IKKγ. The latter protein is essential for activation of the IKKs, but its mechanism of action is not known. Here we describe the molecular cloning of CIKS (connection to IKK and SAPK/JNK), a previously unknown protein that directly interacts with NEMO/IKKγ in cells. When ectopically expressed, CIKS stimulates IKK and SAPK/JNK kinases and it transactivates an NF-κB-dependent reporter. Activation of NF-κB is prevented in the presence of kinase-deficient, interfering mutants of the IKKs. CIKS may help to connect upstream signaling events to IKK and SAPK/JNK modules. CIKS could coordinate the activation of two stress-induced signaling pathways, functions reminiscent of those noted for tumor necrosis factor receptor-associated factor adaptor proteins. PMID:10962033

  13. Power plant system assessment. Final report. SP-100 Program

    International Nuclear Information System (INIS)

    Anderson, R.V.; Atkins, D.F.; Bost, D.S.

    1983-01-01

    The purpose of this assessment was to provide system-level insights into 100-kWe-class space reactor electric systems. Using these insights, Rockwell was to select and perform conceptual design studies on a ''most attractive'' system that met the preliminary design goals and requirements of the SP-100 Program. About 4 of the 6 months were used in the selection process. The remaining 2 months were used for the system conceptual design studies. Rockwell completed these studies at the end of FY 1983. This report summarizes the results of the power plant system assessment and describes our choice for the most attractive system - the Rockwell SR-100G System (Space Reactor, 100 kWe, Growth) - a lithium-cooled UN-fueled fast reactor/Brayton turboelectric converter system

  14. Implementing agreement on photovoltaic power systems - Annual report 2009

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2010-07-01

    This annual report for the International Energy Agency (IEA) reports on the programme's activities in 2009. The IEA Photovoltaic Power Systems Programme (PVPS) is one of the collaborative research and development agreements established within the IEA. Its mission is to enhance international collaboration efforts which support the development and deployment of photovoltaic solar energy. In this annual report, the programme's mission and its strategies for reaching four objectives are reviewed and status reports on the programme's various tasks and sub-tasks are presented, as are activities planned for 2010. The tasks include the exchange and dissemination of information on photovoltaic power systems, a study on very large scale photovoltaic power generation system, photovoltaic services for developing countries, urban-scale PV applications, hybrid systems within mini-grids, PV environmental health and safety activities, performance and reliability of PV systems and high penetration PV in electricity grids. The status and prospects in the 23 countries and organisations participating in the programme are presented. Along with country-specific topics, industry activities, research, development and demonstration projects, applications, education and governmental activities as well as future activities are reviewed. Finally, completed tasks are reviewed. These include the performance, reliability and analysis of photovoltaic systems, the use of photovoltaic power systems in stand-alone and island applications, grid interconnection of building integrated and other distributed photovoltaic power systems, design and operation of modular photovoltaic plants for large scale power generation and photovoltaic power systems in the built environment. The report is completed with a list of Executive Committee members and Operating Agents.

  15. Downstream of tyrosine kinase/docking protein 6, as a novel substrate of tropomyosin-related kinase C receptor, is involved in neurotrophin 3-mediated neurite outgrowth in mouse cortex neurons

    Directory of Open Access Journals (Sweden)

    Yuan Jian

    2010-06-01

    Full Text Available Abstract Background The downstream of tyrosine kinase/docking protein (Dok adaptor protein family has seven members, Dok1 to Dok7, that act as substrates of multiple receptor tyrosine kinase and non-receptor tyrosine kinase. The tropomyosin-related kinase (Trk receptor family, which has three members (TrkA, TrkB and TrkC, are receptor tyrosine kinases that play pivotal roles in many stages of nervous system development, such as differentiation, migration, axon and dendrite projection and neuron patterning. Upon related neurotrophin growth factor stimulation, dimerisation and autophosphorylation of Trk receptors can occur, recruiting adaptor proteins to mediate signal transduction. Results In this report, by using yeast two-hybrid assays, glutathione S-transferase (GST precipitation assays and coimmunoprecipitation (Co-IP experiments, we demonstrate that Dok6 selectively binds to the NPQY motif of TrkC through its phosphotyrosine-binding (PTB domain in a kinase activity-dependent manner. We further confirmed their interaction by coimmunoprecipitation and colocalisation in E18.5 mouse cortex neurons, which provided more in vivo evidence. Next, we demonstrated that Dok6 is involved in neurite outgrowth in mouse cortex neurons via the RNAi method. Knockdown of Dok6 decreased neurite outgrowth in cortical neurons upon neurotrophin 3 (NT-3 stimulation. Conclusions We conclude that Dok6 interacts with the NPQY motif of the TrkC receptor through its PTB domain in a kinase activity-dependent manner, and works as a novel substrate of the TrkC receptor involved in NT-3-mediated neurite outgrowth in mouse cortex neurons.

  16. Status Report on Structural Materials for Advanced Nuclear Systems

    International Nuclear Information System (INIS)

    Allen, T.R.; Balbaud-Celerier, F.; Asayama, T.; Pouchon, M.; Busby, J.T.; Maloy, S.; Park, J.Y.; Fazio, C.; Dai, Y.; Agostini, P.; Chevalier, J.P.; Marrow, J.

    2013-01-01

    Materials performance is critical to the safe and economic operation of any nuclear system. As the international community pursues the development of Generation IV reactor concepts and accelerator-driven transmutation systems, it will be increasingly necessary to develop advanced materials capable of tolerating the more challenging environments of these new systems. The international community supports numerous materials research programmes, with each country determining its individual focus on a case-by-case basis. In many instances, similar alloys of materials systems are being studied in several countries, providing the opportunity for collaborative and cross-cutting research that benefits different systems. This report is a snapshot of the current materials programmes supporting the development of advanced concepts. The descriptions of the research are grouped by concept, and national programmes are described within each concept. The report provides an overall sense of the importance of materials research worldwide and the opportunities for synergy among the countries represented in this overview. (authors)

  17. Automated system for recording reports in nuclear medicine

    Energy Technology Data Exchange (ETDEWEB)

    Machida, K; Hayashi, S; Watari, T; Akaike, A; Oyama, K [Tokyo Univ. (Japan). Faculty of Medicine

    1976-07-01

    In order to overcome the rapid expansion of nuclear medicine as a diagnostic tool, we developed the system called ''Radiological Bunin Report System'' to record the clinical report in nuclear medicine automatically using the Tosbac 40 time sharing system electronic computer and the keymat-editor (DTZ 0008A). The system is composed of three modules, that is, reader, editor and writer. The module of the reader is used to register sentences and terms by which one can easily add to or change the registered dictionary. The module of the editor is used to make an intermediate file of radiological reports. With the aid of a keymateditor a radiologist puts in the necessary sentences and terms usually in the following order: procedure, interpretation, diagnosis, recommendation and doctor's code, thus making the intermediate file. In this procedure error message, if any, may be printed out on terminal typewriter. Finally the module of the writer is used to edit the intermediate file and to make sentences. Having stored several clinical reports, the computer automatically produces as many of the English written reports as one wants by printing order. By this system we are able to save man power in the hospital, and the radiological report is standardized. Furthermore, in view of the rapid progress and change of nuclear medicine, it is very important that a radiologist is able to change and add sentences and terms on a keymat very easily so that the system may be kept up-to-date and valuable. We believe this is one of the most characteristic advantages of the system.

  18. Automated system for recording reports in nuclear medicine

    International Nuclear Information System (INIS)

    Machida, Kikuo; Hayashi, Sanshin; Watari, Tsutomu; Akaike, Akira; Oyama, Kazuyuki

    1976-01-01

    In order to overcome the rapid expansion of nuclear medicine as a diagnostic tool, we developed the system called ''Radiological Bunin Report System'' to record the clinical report in nuclear medicine automatically using the Tosbac 40 time sharing system electronic computer and the keymat-editor (DTZ 0008A). The system is composed of three modules, that is, reader, editor and writer. The module of the reader is used to register sentences and terms by which one can easily add to or change the registered dictionary. The module of the editor is used to make an intermediate file of radiological reports. With the aid of a keymateditor a radiologist puts in the necessary sentences and terms usually in the following order: procedure, interpretation, diagnosis, recommendation and doctor's code, thus making the intermediate file. In this procedure error message, if any, may be printed out on terminal typewriter. Finally the module of the writer is used to edit the intermediate file and to make sentences. Having stored several clinical reports, the computer automatically produces as many of the English written reports as one wants by printing order. By this system we are able to save man power in the hospital, and the radiological report is standardized. Furthermore, in view of the rapid progress and change of nuclear medicine, it is very important that a radiologist is able to change and add sentences and terms on a keymat very easily so that the system may be kept up-to-date and valuable. We believe this is one of the most characteristic advantages of the system. (auth.)

  19. 296-B-5 Stack monitoring and sampling system annual system assessment report

    International Nuclear Information System (INIS)

    Ridge, T.M.

    1995-02-01

    The B Plant Administration Manual requires an annual system assessment to evaluate and report the present condition of the sampling and monitoring system associated with Stack 296-B-5 at B Plant. The sampling and monitoring system associated with stack 296-B-5 is functional and performing satisfactorily. This document is an annual assessment report of the systems associated with the 296-B-5 stack

  20. Ibrutinib Treatment of Mantle Cell Lymphoma Relapsing at Central Nervous System: A Case Report and Literature Review

    Directory of Open Access Journals (Sweden)

    Donato Mannina

    2017-01-01

    Full Text Available Mantle cell lymphoma (MCL accounts for about 5% of all lymphomas. Its clinical and histological features are heterogeneous. After a frequently good initial response, the disease generally and repeatedly relapses and finally the outcome is poor. Particularly severe is the prognosis of the rare occurrence of CNSi (Central Nervous System involvement. Ibrutinib, an oral inhibitor of Bruton tyrosine kinase (BTK, has shown strong activity in relapsing patients with Chronic Lymphocytic Leukemia (CLL and MCL. Few reports are available about treatment with ibrutinib of patients presenting CNSi by lymphoproliferative diseases (LPD. In all of them, ibrutinib, at the dosage between 420 and 560 mg/day, showed an impressive effectiveness. Here we describe a case of MCL with CNS relapse showing an excellent response to ibrutinib administered at the unusual dose of 280 mg/day because of concomitant treatment of cardiological disease.

  1. Systems biology approach identifies the kinase Csnk1a1 as a regulator of the DNA damage response in embryonic stem cells.

    Science.gov (United States)

    Carreras Puigvert, Jordi; von Stechow, Louise; Siddappa, Ramakrishnaiah; Pines, Alex; Bahjat, Mahnoush; Haazen, Lizette C J M; Olsen, Jesper V; Vrieling, Harry; Meerman, John H N; Mullenders, Leon H F; van de Water, Bob; Danen, Erik H J

    2013-01-22

    In pluripotent stem cells, DNA damage triggers loss of pluripotency and apoptosis as a safeguard to exclude damaged DNA from the lineage. An intricate DNA damage response (DDR) signaling network ensures that the response is proportional to the severity of the damage. We combined an RNA interference screen targeting all kinases, phosphatases, and transcription factors with global transcriptomics and phosphoproteomics to map the DDR in mouse embryonic stem cells treated with the DNA cross-linker cisplatin. Networks derived from canonical pathways shared in all three data sets were implicated in DNA damage repair, cell cycle and survival, and differentiation. Experimental probing of these networks identified a mode of DNA damage-induced Wnt signaling that limited apoptosis. Silencing or deleting the p53 gene demonstrated that genotoxic stress elicited Wnt signaling in a p53-independent manner. Instead, this response occurred through reduced abundance of Csnk1a1 (CK1α), a kinase that inhibits β-catenin. Together, our findings reveal a balance between p53-mediated elimination of stem cells (through loss of pluripotency and apoptosis) and Wnt signaling that attenuates this response to tune the outcome of the DDR.

  2. Development of Spreadsheet-Based Integrated Transaction Processing Systems and Financial Reporting Systems

    Science.gov (United States)

    Ariana, I. M.; Bagiada, I. M.

    2018-01-01

    Development of spreadsheet-based integrated transaction processing systems and financial reporting systems is intended to optimize the capabilities of spreadsheet in accounting data processing. The purpose of this study are: 1) to describe the spreadsheet-based integrated transaction processing systems and financial reporting systems; 2) to test its technical and operational feasibility. This study type is research and development. The main steps of study are: 1) needs analysis (need assessment); 2) developing spreadsheet-based integrated transaction processing systems and financial reporting systems; and 3) testing the feasibility of spreadsheet-based integrated transaction processing systems and financial reporting systems. The technical feasibility include the ability of hardware and operating systems to respond the application of accounting, simplicity and ease of use. Operational feasibility include the ability of users using accounting applications, the ability of accounting applications to produce information, and control applications of the accounting applications. The instrument used to assess the technical and operational feasibility of the systems is the expert perception questionnaire. The instrument uses 4 Likert scale, from 1 (strongly disagree) to 4 (strongly agree). Data were analyzed using percentage analysis by comparing the number of answers within one (1) item by the number of ideal answer within one (1) item. Spreadsheet-based integrated transaction processing systems and financial reporting systems integrate sales, purchases, and cash transaction processing systems to produce financial reports (statement of profit or loss and other comprehensive income, statement of changes in equity, statement of financial position, and statement of cash flows) and other reports. Spreadsheet-based integrated transaction processing systems and financial reporting systems is feasible from the technical aspects (87.50%) and operational aspects (84.17%).

  3. Classification system for reporting events involving human malfunctions

    International Nuclear Information System (INIS)

    Rasmussen, J.; Pedersen, O.M.; Mancini, G.

    1981-01-01

    The report describes a set of categories for reporting industrial incidents and events involving human malfunction. The classification system aims at ensuring information adequate for improvement of human work situations and man-machine interface systems and for attempts to quantify ''human error'' rates. The classification system has a multifacetted non-hierarchical structure and its compatibility with Ispra's ERDS classification is described. The collection of the information in general and for quantification purposes are discussed. 24 categories, 12 of which being human factors-oriented, are listed with their respective subcategories, and comments are given. Underlying models of human data process and their typical malfuntions and of a human decision sequence are described. The work reported is a joint contribution to the CSNI Group of Experts on Human Error Data and Assessment

  4. Discriminator/time interval meter system evaluation report

    International Nuclear Information System (INIS)

    Condreva, K.J.

    1976-01-01

    The purpose of this report is to discuss the evaluation of a modular prototype Discriminator/Time Interval Meter data acquisition unit as a useful tool in a digital diagnostics system. The characteristics, operation and calibration of each of the hardware components are discussed in some detail. A discussion of the system calibration, operation, and data ingestion and reduction is also given. System test results to date are given and discussed. Finally, recommendations and conclusions concerning the capabilities of the Discriminator/T.I.M. system based on test and calibration results to date are given

  5. Status report on the Advanced Light Source control system, 1993

    International Nuclear Information System (INIS)

    Young, J.; Brown, W. Jr.; Cork, C.

    1993-10-01

    The Advanced Light Source (ALS), under construction for the past seven years, has become operational. The accelerator has been successfully commissioned using a control system based on hundreds of controllers of our own design and high performance personal computers which are the operator interface. The first beamlines are being commissioned using a control system based on VME hardware and the Experimental Physics and Industrial Control System (EPICS) software. The two systems are being integrated, and this paper reports on the current work being done

  6. Discriminator/time interval meter system evaluation report

    Energy Technology Data Exchange (ETDEWEB)

    Condreva, K. J.

    1976-04-12

    The purpose of this report is to discuss the evaluation of a modular prototype Discriminator/Time Interval Meter data acquisition unit as a useful tool in a digital diagnostics system. The characteristics, operation and calibration of each of the hardware components are discussed in some detail. A discussion of the system calibration, operation, and data ingestion and reduction is also given. System test results to date are given and discussed. Finally, recommendations and conclusions concerning the capabilities of the Discriminator/T.I.M. system based on test and calibration results to date are given.

  7. Upgrading the Fermilab fire and security reporting system

    International Nuclear Information System (INIS)

    King, C.; Neswold, R.

    2012-01-01

    Fermilab's home grown fire and security system (known as FIRUS - Fire Incident Reporting and Utility System) is highly reliable and has been used for nearly thirty years. The system has gone through some minor upgrades, however, none of those changes made significant, visible changes. In this paper, we present a major overhaul to the system that is halfway complete. We discuss the use of Apple's OS X for the new GUI (Graphical User Interface), upgrading the servers to use the Erlang programming language and allowing limited access for iOS and Android-based mobile devices. (authors)

  8. B Plant low level waste system integrity assessment report

    International Nuclear Information System (INIS)

    Walter, E.J.

    1995-09-01

    This document provides the report of the integrity assessment activities for the B Plant low level waste system. The assessment activities were in response to requirements of the Washington State Dangerous Waste Regulations, Washington Administrative Code (WAC), 173-303-640. This integrity assessment report supports compliance with Hanford Federal Facility Agreement and Consent Order interim milestone target action M-32-07-T03

  9. Supplementary report: cooling water systems for Darlington G.S

    International Nuclear Information System (INIS)

    1975-08-01

    This report summarizes Ontario Hydro's existing aquatic environmental programs, presents results of these investigations, and outlines plans and activities for expanded aquatic environment studies including the evaluation of alternative cooling systems. This report outlines specific considerations regarding possible alternative cooling arrangements for the Darlington station. It concludes with a recommendation that a study be initiated to examine the potential benefits of using the heated discharge water in a warm water recreational centre. (author)

  10. Preliminary design report for the prototypical fuel rod consolidation system

    International Nuclear Information System (INIS)

    Rosa, J.M.

    1986-01-01

    This report documents NUTECH's preliminary design of a dry, spent fuel rod consolidation system. This preliminary design is the result of Phase I of a planned four phase project. The present report on this project provides a considerable amount of detail for a preliminary design effort. The design and all of its details are described in this Preliminary Design Report (PDR). The NUTECH dry rod consolidation system described herein is remotely operated. It provides for automatic operation, but with operator hold points between key steps in the process. The operator has the ability to switch to a manual operation mode at any point in the process. The system is directed by the operator using an executive computer which controls and coordinates the operation of the in-cell equipment. The operator monitors the process using an in-cell closed circuit television (CCTV) system with audio output and equipment status displays on the computer monitor. The in-cell mechanical equipment consists of the following: (1) two overhead cranes with manipulators; (2) a multi-degree of freedom fuel handling table and its clamping equipment; (3) a fuel assembly end fitting removal station and its tools; (4) a consolidator (which pulls rods, assembles the consolidated bundle and loads the canister); (5) a canister end cap welder and weld inspection system; (6) decontamination systems; and (7) the CCTV and microphone systems

  11. Developing standard transmission system for radiology reporting including key images

    International Nuclear Information System (INIS)

    Kim, Seon Chil

    2007-01-01

    Development of hospital information system and Picture Archiving Communication System is not new in the medical field, and the development of internet and information technology are also universal. In the course of such development, however, it is hard to share medical information without a refined standard format. Especially in the department of radiology, the role of PACS has become very important in interchanging information with other disparate hospital information systems. A specific system needs to be developed that radiological reports are archived into a database efficiently. This includes sharing of medical images. A model is suggested in this study in which an internal system is developed where radiologists store necessary images and transmit them is the standard international clinical format, Clinical Document Architecture, and share the information with hospitals. CDA document generator was made to generate a new file format and separate the existing storage system from the new system. This was to ensure the access to required data in XML documents. The model presented in this study added a process where crucial images in reading are inserted in the CDA radiological report generator. Therefore, this study suggests a storage and transmission model for CDA documents, which is different from the existing DICOM SR. Radiological reports could be better shared, when the application function for inserting images and the analysis of standard clinical terms are completed

  12. Behavioural Verification: Preventing Report Fraud in Decentralized Advert Distribution Systems

    Directory of Open Access Journals (Sweden)

    Stylianos S. Mamais

    2017-11-01

    Full Text Available Service commissions, which are claimed by Ad-Networks and Publishers, are susceptible to forgery as non-human operators are able to artificially create fictitious traffic on digital platforms for the purpose of committing financial fraud. This places a significant strain on Advertisers who have no effective means of differentiating fabricated Ad-Reports from those which correspond to real consumer activity. To address this problem, we contribute an advert reporting system which utilizes opportunistic networking and a blockchain-inspired construction in order to identify authentic Ad-Reports by determining whether they were composed by honest or dishonest users. What constitutes a user’s honesty for our system is the manner in which they access adverts on their mobile device. Dishonest users submit multiple reports over a short period of time while honest users behave as consumers who view adverts at a balanced pace while engaging in typical social activities such as purchasing goods online, moving through space and interacting with other users. We argue that it is hard for dishonest users to fake honest behaviour and we exploit the behavioural patterns of users in order to classify Ad-Reports as real or fabricated. By determining the honesty of the user who submitted a particular report, our system offers a more secure reward-claiming model which protects against fraud while still preserving the user’s anonymity.

  13. Nuclear-power-safety reporting system: feasibility analysis

    International Nuclear Information System (INIS)

    Finlayson, F.C.; Ims, J.

    1983-04-01

    The US Nuclear Regulatory Commission (NRC) is evaluating the possibility of instituting a data gathering system for identifying and quantifying the factors that contribute to the occurrence of significant safety problems involving humans in nuclear power plants. This report presents the results of a brief (6 months) study of the feasibility of developing a voluntary, nonpunitive Nuclear Power Safety Reporting System (NPSRS). Reports collected by the system would be used to create a data base for documenting, analyzing and assessing the significance of the incidents. Results of The Aerospace Corporation study are presented in two volumes. This document, Volume I, contains a summary of an assessment of the Aviation Safety Reporting System (ASRS). The FAA-sponsored, NASA-managed ASRS was found to be successful, relatively low in cost, generally acceptable to all facets of the aviation community, and the source of much useful data and valuable reports on human factor problems in the nation's airways. Several significant ASRS features were found to be pertinent and applicable for adoption into a NPSRS

  14. National high-level waste systems analysis report

    Energy Technology Data Exchange (ETDEWEB)

    Kristofferson, K.; Oholleran, T.P.; Powell, R.H.

    1995-09-01

    This report documents the assessment of budgetary impacts, constraints, and repository availability on the storage and treatment of high-level waste and on both existing and pending negotiated milestones. The impacts of the availabilities of various treatment systems on schedule and throughput at four Department of Energy sites are compared to repository readiness in order to determine the prudent application of resources. The information modeled for each of these sites is integrated with a single national model. The report suggests a high-level-waste model that offers a national perspective on all high-level waste treatment and storage systems managed by the Department of Energy.

  15. National high-level waste systems analysis report

    International Nuclear Information System (INIS)

    Kristofferson, K.; Oholleran, T.P.; Powell, R.H.

    1995-09-01

    This report documents the assessment of budgetary impacts, constraints, and repository availability on the storage and treatment of high-level waste and on both existing and pending negotiated milestones. The impacts of the availabilities of various treatment systems on schedule and throughput at four Department of Energy sites are compared to repository readiness in order to determine the prudent application of resources. The information modeled for each of these sites is integrated with a single national model. The report suggests a high-level-waste model that offers a national perspective on all high-level waste treatment and storage systems managed by the Department of Energy

  16. Recognition of medical errors' reporting system dimensions in educational hospitals.

    Science.gov (United States)

    Yarmohammadian, Mohammad H; Mohammadinia, Leila; Tavakoli, Nahid; Ghalriz, Parvin; Haghshenas, Abbas

    2014-01-01

    Nowadays medical errors are one of the serious issues in the health-care system and carry to account of the patient's safety threat. The most important step for achieving safety promotion is identifying errors and their causes in order to recognize, correct and omit them. Concerning about repeating medical errors and harms, which were received via theses errors concluded to designing and establishing medical error reporting systems for hospitals and centers that are presenting therapeutic services. The aim of this study is the recognition of medical errors' reporting system dimensions in educational hospitals. This research is a descriptive-analytical and qualities' study, which has been carried out in Shahid Beheshti educational therapeutic center in Isfahan during 2012. In this study, relevant information was collected through 15 face to face interviews. That each of interviews take place in about 1hr and creation of five focused discussion groups through 45 min for each section, they were composed of Metron, educational supervisor, health officer, health education, and all of the head nurses. Concluded data interviews and discussion sessions were coded, then achieved results were extracted in the presence of clear-sighted persons and after their feedback perception, they were categorized. In order to make sure of information correctness, tables were presented to the research's interviewers and final the corrections were confirmed based on their view. The extracted information from interviews and discussion groups have been divided into nine main categories after content analyzing and subject coding and their subsets have been completely expressed. Achieved dimensions are composed of nine domains of medical error concept, error cases according to nurses' prospection, medical error reporting barriers, employees' motivational factors for error reporting, purposes of medical error reporting system, error reporting's challenges and opportunities, a desired system

  17. A-Raf kinase is a new interacting partner of protein kinase CK2 beta subunit

    DEFF Research Database (Denmark)

    Boldyreff, B; Issinger, O G

    1997-01-01

    In a search for protein kinase CK2 beta subunit binding proteins using the two-hybrid system, more than 1000 positive clones were isolated. Beside clones for the alpha' and beta subunit of CK2, there were clones coding for a so far unknown protein, whose partial cDNA sequence was already deposited...

  18. 242A Distributed Control System Year 2000 Acceptance Test Report

    Energy Technology Data Exchange (ETDEWEB)

    TEATS, M.C.

    1999-08-31

    This report documents acceptance test results for the 242-A Evaporator distributive control system upgrade to D/3 version 9.0-2 for year 2000 compliance. This report documents the test results obtained by acceptance testing as directed by procedure HNF-2695. This verification procedure will document the initial testing and evaluation of the potential 242-A Distributed Control System (DCS) operating difficulties across the year 2000 boundary and the calendar adjustments needed for the leap year. Baseline system performance data will be recorded using current, as-is operating system software. Data will also be collected for operating system software that has been modified to correct year 2000 problems. This verification procedure is intended to be generic such that it may be performed on any D/3{trademark} (GSE Process Solutions, Inc.) distributed control system that runs with the VMSTM (Digital Equipment Corporation) operating system. This test may be run on simulation or production systems depending upon facility status. On production systems, DCS outages will occur nine times throughout performance of the test. These outages are expected to last about 10 minutes each.

  19. 242A Distributed Control System Year 2000 Acceptance Test Report

    International Nuclear Information System (INIS)

    TEATS, M.C.

    1999-01-01

    This report documents acceptance test results for the 242-A Evaporator distributive control system upgrade to D/3 version 9.0-2 for year 2000 compliance. This report documents the test results obtained by acceptance testing as directed by procedure HNF-2695. This verification procedure will document the initial testing and evaluation of the potential 242-A Distributed Control System (DCS) operating difficulties across the year 2000 boundary and the calendar adjustments needed for the leap year. Baseline system performance data will be recorded using current, as-is operating system software. Data will also be collected for operating system software that has been modified to correct year 2000 problems. This verification procedure is intended to be generic such that it may be performed on any D/3(trademark) (GSE Process Solutions, Inc.) distributed control system that runs with the VMSTM (Digital Equipment Corporation) operating system. This test may be run on simulation or production systems depending upon facility status. On production systems, DCS outages will occur nine times throughout performance of the test. These outages are expected to last about 10 minutes each

  20. Structure of the intact ATM/Tel1 kinase

    Science.gov (United States)

    Wang, Xuejuan; Chu, Huanyu; Lv, Mengjuan; Zhang, Zhihui; Qiu, Shuwan; Liu, Haiyan; Shen, Xuetong; Wang, Weiwu; Cai, Gang

    2016-05-01

    The ataxia-telangiectasia mutated (ATM) protein is an apical kinase that orchestrates the multifaceted DNA-damage response. Normally, ATM kinase is in an inactive, homodimer form and is transformed into monomers upon activation. Besides a conserved kinase domain at the C terminus, ATM contains three other structural modules, referred to as FAT, FATC and N-terminal helical solenoid. Here we report the first cryo-EM structure of ATM kinase, which is an intact homodimeric ATM/Tel1 from Schizosaccharomyces pombe. We show that two monomers directly contact head-to-head through the FAT and kinase domains. The tandem N-terminal helical solenoid tightly packs against the FAT and kinase domains. The structure suggests that ATM/Tel1 dimer interface and the consecutive HEAT repeats inhibit the binding of kinase substrates and regulators by steric hindrance. Our study provides a structural framework for understanding the mechanisms of ATM/Tel1 regulation as well as the development of new therapeutic agents.

  1. Receptor-interacting protein (RIP) kinase family

    OpenAIRE

    Zhang, Duanwu; Lin, Juan; Han, Jiahuai

    2010-01-01

    Receptor-interacting protein (RIP) kinases are a group of threonine/serine protein kinases with a relatively conserved kinase domain but distinct non-kinase regions. A number of different domain structures, such as death and caspase activation and recruitment domain (CARD) domains, were found in different RIP family members, and these domains should be keys in determining the specific function of each RIP kinase. It is known that RIP kinases participate in different biological processes, incl...

  2. Nuclear power safety reporting system feasibility analysis and concept description

    International Nuclear Information System (INIS)

    Finlayson, F.C.; Ims, J.R.; Hussman, T.A.

    1984-01-01

    The Aerospace Corporation is assisting the US Nuclear Regulatory Commission (NRC) in the evaluation of the potential attributes of a voluntary, nonpunitive data gathering system for identifying and quantifying the factors that contribute to the occurrence of significant safety problems involving humans in nuclear power plants. The objectives of the Aerospace Administration (FAA)/National Aeronautics and Space Administration (NASA) Aviation Safety Reporting System (ASRS) in order to determine whether it would be feasible to apply part (or all) of the ASRS concepts for collecting data on human factor related incidents to the nuclear industry; and (2) to identify and define the basic elements and requirements of a Nuclear Power Safety Reporting System (NPSRS), assuming the feasibility of implementing such a system was established

  3. Computerized management report system for monitoring manpower and cost

    International Nuclear Information System (INIS)

    Bullington, V.R.; Stephenson, R.L.; Cardwell, R.G.

    1980-04-01

    Although most cost systems offer complete detail and traceability, not all provide timely detail in a concise form useful to senior management. This system was developed for a multifunction research organization funded from many sources. It extracts cost and manpower data from the general cost systems, summarizes it, compares it by program with previous cost periods, and presents it with minimum detail yet with maximum overview. The system monitors the basic manpower distribution of effort at the source, that is, the division time-card input. Cost data are taken from the central computer ahead of the print-out and report-distribution steps; thus, the summary information is available several days ahead of the detailed reports. This procedure has been regularly used for several months, and has proven to be a valuable tool in management action and planning. 9 figures

  4. Report on task I: fire protection system study

    International Nuclear Information System (INIS)

    Bernard, E.A.; Cano, G.L.

    1977-02-01

    This study (1) evaluates, on a comparative basis, the national and international regulatory and insurance standards that serve as guidance for fire protection within the nuclear power industry; (2) analyzes the recommendations contained in the major reports on the Browns Ferry Fire; (3) proposes quantitative safety goals and evaluation methods for Nuclear Power Plant Fire Protection Systems (NPPFPS); (4) identifies potential improvements that may be incorporated into NPPFPS; and (5) recommends a plan of action for continuation of the fire protections systems study

  5. Language of Czech Medical Reports and Classification Systems in Medicine

    Czech Academy of Sciences Publication Activity Database

    Přečková, Petra

    2010-01-01

    Roč. 6, č. 1 (2010), s. 58-65 ISSN 1801-5603 R&D Projects: GA MŠk(CZ) 1M06014 Institutional research plan: CEZ:AV0Z10300504 Keywords : terminology, * synonyms * classification systems * thesaurus * nomenclature * electronic health record * interoperability * semantic interoperability * cardiology * atherosclerosis Subject RIV: IN - Informatics, Computer Science http://www.ejbi.org/en/ejbi/article/53-en-language-of-czech-medical-reports- and -classification-systems-in-medicine.html

  6. System Advisor Model: Flat Plate Photovoltaic Performance Modeling Validation Report

    Energy Technology Data Exchange (ETDEWEB)

    Freeman, Janine [National Renewable Energy Lab. (NREL), Golden, CO (United States); Whitmore, Jonathan [National Renewable Energy Lab. (NREL), Golden, CO (United States); Kaffine, Leah [National Renewable Energy Lab. (NREL), Golden, CO (United States); Blair, Nate [National Renewable Energy Lab. (NREL), Golden, CO (United States); Dobos, Aron P. [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2013-12-01

    The System Advisor Model (SAM) is a free software tool that performs detailed analysis of both system performance and system financing for a variety of renewable energy technologies. This report provides detailed validation of the SAM flat plate photovoltaic performance model by comparing SAM-modeled PV system generation data to actual measured production data for nine PV systems ranging from 75 kW to greater than 25 MW in size. The results show strong agreement between SAM predictions and field data, with annualized prediction error below 3% for all fixed tilt cases and below 8% for all one axis tracked cases. The analysis concludes that snow cover and system outages are the primary sources of disagreement, and other deviations resulting from seasonal biases in the irradiation models and one axis tracking issues are discussed in detail.

  7. Plutonium Protection System (PPS). Volume 2. Hardware description. Final report

    International Nuclear Information System (INIS)

    Miyoshi, D.S.

    1979-05-01

    The Plutonium Protection System (PPS) is an integrated safeguards system developed by Sandia Laboratories for the Department of Energy, Office of Safeguards and Security. The system is designed to demonstrate and test concepts for the improved safeguarding of plutonium. Volume 2 of the PPS final report describes the hardware elements of the system. The major areas containing hardware elements are the vault, where plutonium is stored, the packaging room, where plutonium is packaged into Container Modules, the Security Operations Center, which controls movement of personnel, the Material Accountability Center, which maintains the system data base, and the Material Operations Center, which monitors the operating procedures in the system. References are made to documents in which details of the hardware items can be found

  8. Oak Ridge Reservation Public Warning Siren System Annual Test Report

    International Nuclear Information System (INIS)

    R. F. Gee

    2000-01-01

    The full operational test of the Oak Ridge Reservation (ORR) Public Warning Siren System (PWSS) was successfully conducted on September 27, 2000. The annual test is a full-scale sounding of the individual siren systems around each of the three Department of Energy (DOE) sites in Oak Ridge, Tennessee. The purpose of the annual test is to demonstrate and validate the siren systems' ability to alert personnel outdoors in the Immediate Notification Zones (INZ) (approximately two miles) around each site. The success of this test is based on two critical functions of the siren system. The first function is system operability. The system is considered operable if 90% of the sirens are operational. System diagnostics and direct field observations were used to validate the operability of the siren systems. Based on the diagnostic results and field observations, greater than 90% of the sirens were considered operational. The second function is system audibility. The system is considered audible if the siren could be heard in the immediate notification zones around each of the three sites. Direct field observations, along with sound level measurements, were used to validate the audibility of the siren system. Based on the direct field observations and sound level measurements, the siren system was considered audible. The combination of field observations, system diagnostic status reports, and sound level measurements provided a high level of confidence that the system met and would meet operational requirements upon demand. As part of the overall system test, the Tennessee Emergency Management Agency (TEMA) activated the Emergency Alerting System (EAS), which utilized area radio stations to make announcements regarding the test and to remind residents of what to do in the event of an actual emergency

  9. EMS helicopter incidents reported to the NASA Aviation Safety Reporting System

    Science.gov (United States)

    Connell, Linda J.; Reynard, William D.

    1993-01-01

    The objectives of this evaluation were to: Identify the types of safety-related incidents reported to the Aviation Safety Reporting System (ASRS) in Emergency Medical Service (EMS) helicopter operations; Describe the operational conditions surrounding these incidents, such as weather, airspace, flight phase, time of day; and Assess the contribution to these incidents of selected human factors considerations, such as communication, distraction, time pressure, workload, and flight/duty impact.

  10. Technology verification phase. Dynamic isotope power system. Final report

    International Nuclear Information System (INIS)

    Halsey, D.G.

    1982-01-01

    The Phase I requirements of the Kilowatt Isotope Power System (KIPS) program were to make a detailed Flight System Conceptual Design (FSCD) for an isotope fueled organic Rankine cycle power system and to build and test a Ground Demonstration System (GDS) which simulated as closely as possible the operational characteristics of the FSCD. The activities and results of Phase II, the Technology Verification Phase, of the program are reported. The objectives of this phase were to increase system efficiency to 18.1% by component development, to demonstrate system reliability by a 5000 h endurance test and to update the flight system design. During Phase II, system performance was improved from 15.1% to 16.6%, an endurance test of 2000 h was performed while the flight design analysis was limited to a study of the General Purpose Heat Source, a study of the regenerator manufacturing technique and analysis of the hardness of the system to a laser threat. It was concluded from these tests that the GDS is basically prototypic of a flight design; all components necessary for satisfactory operation were demonstrated successfully at the system level; over 11,000 total h of operation without any component failure attested to the inherent reliability of this type of system; and some further development is required, specifically in the area of performance

  11. Technology verification phase. Dynamic isotope power system. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Halsey, D.G.

    1982-03-10

    The Phase I requirements of the Kilowatt Isotope Power System (KIPS) program were to make a detailed Flight System Conceptual Design (FSCD) for an isotope fueled organic Rankine cycle power system and to build and test a Ground Demonstration System (GDS) which simulated as closely as possible the operational characteristics of the FSCD. The activities and results of Phase II, the Technology Verification Phase, of the program are reported. The objectives of this phase were to increase system efficiency to 18.1% by component development, to demonstrate system reliability by a 5000 h endurance test and to update the flight system design. During Phase II, system performance was improved from 15.1% to 16.6%, an endurance test of 2000 h was performed while the flight design analysis was limited to a study of the General Purpose Heat Source, a study of the regenerator manufacturing technique and analysis of the hardness of the system to a laser threat. It was concluded from these tests that the GDS is basically prototypic of a flight design; all components necessary for satisfactory operation were demonstrated successfully at the system level; over 11,000 total h of operation without any component failure attested to the inherent reliability of this type of system; and some further development is required, specifically in the area of performance. (LCL)

  12. Operating Experience from Events Reported to the IAEA Incident Reporting System for Research Reactors

    International Nuclear Information System (INIS)

    2015-03-01

    Operating experience feedback is an effective mechanism in providing lessons learned from events and the associated corrective actions to prevent them, helping to improve safety at nuclear installations. The Incident Reporting System for Research Reactors (IRSRR), which is operated by the IAEA, is an important tool for international exchange of operating experience feedback for research reactors. The IRSRR reports contain information on events of safety significance with their root causes and lessons learned which help in reducing the occurrence of similar events at research reactors. To improve the effectiveness of the system, it is essential that national organizations demonstrate an appropriate interest for the timely reporting of events important to safety and share the information in the IRSRR database. At their biennial technical meetings, the IRSRR national coordinators recommended collecting the operating experience from the events reported to the IRSRR and disseminating it in an IAEA publication. This publication highlights the root causes, safety significance, lessons learned, corrective actions and the causal factors for the events reported to the IRSRR up to September 2014. The publication also contains relevant summary information on research reactor events from sources other than the IRSRR, operating experience feedback from the International Reporting System for Operating Experience considered relevant to research reactors, and a description of the elements of an operating experience programme as established by the IAEA safety standards. This publication will be of use to research reactor operating organizations, regulators and designers, and any other organizations or individuals involved in the safety of research reactors

  13. Disability Evaluation System Analysis and Research Annual Report 2017

    Science.gov (United States)

    2017-11-20

    females, other race, enlisted and active duty service members for all services and time periods. • Rates increase as age increases in the Army. For the...Annual Report 2017 Disability Evaluation Systems Analysis and Research Prepared by Accession Medical Standards Analysis and Research Activity ...50 History of hospitalization among active duty service

  14. Operational test report integrated system test (ventilation upgrade)

    Energy Technology Data Exchange (ETDEWEB)

    HARTY, W.M.

    1999-10-05

    Operational Final Test Report for Integrated Systems, Project W-030 (Phase 2 test, RECIRC and HIGH-HEAT Modes). Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks, including upgraded vapor space cooling and filtered venting of tanks AY101, Ay102, AZ101, AZ102.

  15. Operational test report, integrated system test (ventilation upgrade)

    International Nuclear Information System (INIS)

    HARTY, W.M.

    1999-01-01

    Operational Final Test Report for Integrated Systems, Project W-030 (Phase 2 test, RECIRC and HIGH-HEAT Modes). Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks, including upgraded vapor space cooling and filtered venting of tanks AY101, AY102, AZ101, AZ102

  16. Report of safety of the characterizing system of radioactive waste

    International Nuclear Information System (INIS)

    Angeles C, A.; Jimenez D, J.; Reyes L, J.

    1998-09-01

    Report of safety of the system of radioactive waste of the ININ: Installation, participant personnel, selection of the place, description of the installation, equipment. Proposed activities: operations with radioactive material, calibration in energy, calibration in efficiency, types of waste. Maintenance: handling of radioactive waste, physical safety. Organization: radiological protection, armor-plating, personal dosemeter, risks and emergency plan, environmental impact, medical exams. (Author)

  17. Food micro systems : report on Existing Literature Regarding Consumers' Perception

    NARCIS (Netherlands)

    Fischer, A.R.H.

    2012-01-01

    This report forms part of the deliverables from a project called "FoodMicroSystems" which has received funding from the European Union’s Seventh Framework Programme FP7/2007-2013 under grant agreement n° 287634. The Community is not responsible for any use that might be made of the content of this

  18. Food micro systems : report on Focus Group on Consumers' Acceptance

    NARCIS (Netherlands)

    Fischer, A.R.H.

    2012-01-01

    This report forms part of the deliverables from a project called "FoodMicroSystems" which has received funding from the European Union’s Seventh Framework Programme FP7/2007-2013 under grant agreement n° 287634. The Community is not responsible for any use that might be made of the content of this

  19. Experience gained with Euratom's nuclear materials accounting and reporting system

    International Nuclear Information System (INIS)

    Schmitt, M.; Kschwendt, H.; Maxwell, A.G.; Littlejohn, M.

    1979-01-01

    The entry into force of the Verification Agreement in early 1977, linked to the wish to update the old Euratom System created in 1959, required that a new Euratom system (Community Regulation) be established. The main aspects of this new system, together with the practical experience gained in one and a half years operation, are presented. Certain basic accounting principles incorporated in the Euratom system, which are somewhat different from IAEA principles, are discussed in detail. This includes the notion of accounting date, some correction procedure aspects as well as the continuous updating of the book inventory to the physical reality in form of inventory changes. The effect of these differences when comparing IAEA and Euratom data is also mentioned. Furthermore, certain of the verifications carried out routinely on the operator's reports as well as on the reports submitted by Euratom to IAEA, are described and quantifications are given. Some mention is also made of areas where Euratom's role goes beyond that of the IAEA, i.e. the reporting implications of accounting for material by origin and control of particular use of the materials as well as verification of ore production and processing activities. Finally, improvements and simplifications concerning reports to the IAEA are proposed. (author)

  20. Flight-systems safety program, September 1982. Progress report

    International Nuclear Information System (INIS)

    Bronisz, S.E.

    1983-02-01

    This technical monthly report covers studies related to the use of 238 PuO 2 in radioisotope power systems carried out for the Office of Space Nuclear Projects of the US Department of Energy by Los Alamos National Laboratory. Most of the studies discussed here are ongoing. Results and conclusions described may change as the work continues

  1. Utah System of Higher Education 2015-16 Annual Report

    Science.gov (United States)

    Utah System of Higher Education, 2016

    2016-01-01

    This annual report describes Utah System of Higher Education's progress in the 2015-2016 academic year in the following areas: (1) Strategic plan; (2) Enrollment and completion; (3) Paying for college; (4) Funding higher education; (5) College preparation; (6) Concurrent enrollment and math; (7) Outreach and access; and (8) Industry and the…

  2. Data Systems and Reports as Active Participants in Data Interpretation

    Science.gov (United States)

    Rankin, Jenny Grant

    2016-01-01

    Most data-informed decision-making in education is undermined by flawed interpretations. Educator-driven interventions to improve data use are beneficial but not omnipotent, as data misunderstandings persist at schools and school districts commended for ideal data use support. Meanwhile, most data systems and reports display figures without…

  3. Automated Energy Distribution and Reliability System Status Report

    Energy Technology Data Exchange (ETDEWEB)

    Buche, D. L.; Perry, S.

    2007-10-01

    This report describes Northern Indiana Public Service Co. project efforts to develop an automated energy distribution and reliability system. The purpose of this project was to implement a database-driven GIS solution that would manage all of the company's gas, electric, and landbase objects.

  4. Case Report: An atypical case of systemic lupus erythematosus ...

    African Journals Online (AJOL)

    Background: Systemic lupus erythematosus (SLE) is a multisystem disease that can be a diagnostic conundrum. Case report: We describe a patient who presented with recurrent fleeting exudative and hemorrhagic pleural effusion. It took multiple visits over 3 months and renal biopsy to con rm the diagnosis of SLE.

  5. Youth, Guns, and the Juvenile Justice System. Research Report.

    Science.gov (United States)

    Butts, Jeffrey; Coggeshall, Mark; Gouvis, Caterina; Mears, Daniel; Travis, Jeremy; Waul, Michelle; White, Ruth

    This report documents trends in youth gun violence and the response within the justice system, noting the growing variety of data resources available to investigate the effect of new gun laws on youth, communities, and public safety. The first section reviews recent trends, examining the major wave of gun violence in the United States during the…

  6. The Battle Command Sustainment Support System: Initial Analysis Report

    Science.gov (United States)

    2016-09-01

    products including jet fuels, distillate fuels, residual fuels, automotive gasoline , specified bulk lubricating oils, aircraft engine oils, fuel...contained within this report. 15. SUBJECT TERMS Mission command Software Tactical applications (TacApps) Command post ...computing environment (CPCE) Command post client Battle command sustainment support System (BCS3) Logistics

  7. Automated Energy Distribution and Reliability System (AEDR): Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Buche, D. L.

    2008-07-01

    This report describes Northern Indiana Public Service Co. project efforts to develop an automated energy distribution and reliability system. The purpose of this project was to implement a database-driven GIS solution that would manage all of the company's gas, electric, and landbase objects.

  8. The complexity of patient safety reporting systems in UK dentistry.

    Science.gov (United States)

    Renton, T; Master, S

    2016-10-21

    Since the 'Francis Report', UK regulation focusing on patient safety has significantly changed. Healthcare workers are increasingly involved in NHS England patient safety initiatives aimed at improving reporting and learning from patient safety incidents (PSIs). Unfortunately, dentistry remains 'isolated' from these main events and continues to have a poor record for reporting and learning from PSIs and other events, thus limiting improvement of patient safety in dentistry. The reasons for this situation are complex.This paper provides a review of the complexities of the existing systems and procedures in relation to patient safety in dentistry. It highlights the conflicting advice which is available and which further complicates an overly burdensome process. Recommendations are made to address these problems with systems and procedures supporting patient safety development in dentistry.

  9. Simulated coal gas MCFC power plant system verification. Final report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-07-30

    The objective of the main project is to identify the current developmental status of MCFC systems and address those technical issues that need to be resolved to move the technology from its current status to the demonstration stage in the shortest possible time. The specific objectives are separated into five major tasks as follows: Stack research; Power plant development; Test facilities development; Manufacturing facilities development; and Commercialization. This Final Report discusses the M-C power Corporation effort which is part of a general program for the development of commercial MCFC systems. This final report covers the entire subject of the Unocal 250-cell stack. Certain project activities have been funded by organizations other than DOE and are included in this report to provide a comprehensive overview of the work accomplished.

  10. Energy Storage Systems Program Report for FY98

    Energy Technology Data Exchange (ETDEWEB)

    Butler, P.C.

    1999-04-01

    Sandia National Laboratories, New Mexico, conducts the Energy Storage Systems Program, which is sponsored by the U.S. Department of Energy's Office of Power Technologies. The goal of this program is to collaborate with industry in developing cost-effective electric energy storage systems for many high-value stationary applications. Sandia National Laboratories is responsible for the engineering analyses, contracted development and testing of energy storage components and systems. This report details the technical achievements realized during fiscal year 1998.

  11. Energy storage systems program report for FY97

    Energy Technology Data Exchange (ETDEWEB)

    Butler, P.C.

    1998-08-01

    Sandia National Laboratories, New Mexico, conducts the Energy Storage Systems Program, which is sponsored by the US Department of Energy`s Office of Utility Technologies. The goal of this program is to collaborate with industry in developing cost-effective electric energy storage systems for many high-value stationary applications. Sandia National Laboratories is responsible for the engineering analyses, contracted development, and testing of energy storage components and systems. This report details the technical achievements realized during fiscal year 1997. 46 figs., 20 tabs.

  12. Utility battery storage systems. Program report for FY95

    Energy Technology Data Exchange (ETDEWEB)

    Butler, P.C.

    1996-03-01

    Sandia National Laboratories, New Mexico, conducts the Utility Battery Storage Systems Program, which is sponsored by the U.S. Department of Energy`s Office of Utility Technologies. The goal of this program is to assist industry in developing cost-effective battery systems as a utility resource option by 2000. Sandia is responsible for the engineering analyses, contracted development, and testing of rechargeable batteries and systems for utility energy storage applications. This report details the technical achievements realized during fiscal year 1995.

  13. Energy Storage Systems Program Report for FY99

    Energy Technology Data Exchange (ETDEWEB)

    BOYES,JOHN D.

    2000-06-01

    Sandia National Laboratories, New Mexico, conducts the Energy Storage Systems Program, which is sponsored by the US Department of Energy's Office of Power Technologies. The goal of this program is to develop cost-effective electric energy storage systems for many high-value stationary applications in collaboration with academia and industry. Sandia National Laboratories is responsible for the engineering analyses, contracted development, and testing of energy storage components and systems. This report details the technical achievements realized during fiscal year 1999.

  14. Automated personnel data base system specifications, Task V. Final report

    International Nuclear Information System (INIS)

    Bartley, H.J.; Bocast, A.K.; Deppner, F.O.; Harrison, O.J.; Kraas, I.W.

    1978-11-01

    The full title of this study is 'Development of Qualification Requirements, Training Programs, Career Plans, and Methodologies for Effective Management and Training of Inspection and Enforcement Personnel.' Task V required the development of an automated personnel data base system for NRC/IE. This system is identified as the NRC/IE Personnel, Assignment, Qualifications, and Training System (PAQTS). This Task V report provides the documentation for PAQTS including the Functional Requirements Document (FRD), the Data Requirements Document (DRD), the Hardware and Software Capabilities Assessment, and the Detailed Implementation Schedule. Specific recommendations to facilitate implementation of PAQTS are also included

  15. Plasticity of the PAS domain and a potential role for signal transduction in the histidine kinase DcuS

    NARCIS (Netherlands)

    Etzkorn, M.; Kneuper, H.; Dünnwald, P.; Vijayan, V.; Krämer, J.; Griesinger, C.; Becker, S.; Unden, G.; Baldus, M.

    2008-01-01

    The mechanistic understanding of how membrane-embedded sensor kinases recognize signals and regulate kinase activity is currently limited. Here we report structure-function relationships of the multidomain membrane sensor kinase DcuS using solidstate NMR, structural modeling and mutagenesis.

  16. Report on emergency electrical power supply systems for nuclear fuel cycle and reactor facilities security systems

    International Nuclear Information System (INIS)

    1977-01-01

    The report includes information that will be useful to those responsible for the planning, design and implementation of emergency electric power systems for physical security and special nuclear materials accountability systems. Basic considerations for establishing the system requirements for emergency electric power for security and accountability operations are presented. Methods of supplying emergency power that are available at present and methods predicted to be available in the future are discussed. The characteristics of capacity, cost, safety, reliability and environmental and physical facility considerations of emergency electric power techniques are presented. The report includes basic considerations for the development of a system concept and the preparation of a detailed system design

  17. Report on emergency electrical power supply systems for nuclear fuel cycle and reactor facilities security systems

    Energy Technology Data Exchange (ETDEWEB)

    1977-01-01

    The report includes information that will be useful to those responsible for the planning, design and implementation of emergency electric power systems for physical security and special nuclear materials accountability systems. Basic considerations for establishing the system requirements for emergency electric power for security and accountability operations are presented. Methods of supplying emergency power that are available at present and methods predicted to be available in the future are discussed. The characteristics of capacity, cost, safety, reliability and environmental and physical facility considerations of emergency electric power techniques are presented. The report includes basic considerations for the development of a system concept and the preparation of a detailed system design.

  18. Protein kinase C signaling and cell cycle regulation

    OpenAIRE

    Black, Adrian R.; Black, Jennifer D.

    2013-01-01

    A link between T cell proliferation and the protein kinase C (PKC) family of serine/threonine kinases has been recognized for about thirty years. However, despite the wealth of information on PKC-mediated control of T cell activation, understanding of the effects of PKCs on the cell cycle machinery in this cell type remains limited. Studies in other systems have revealed important cell cycle-specific effects of PKC signaling that can either positively or negatively impact proliferation. Th...

  19. Classification system for reporting events involving human malfunctions

    International Nuclear Information System (INIS)

    Rasmussen, J.; Pedersen, O.M.; Mancini, G.; Carnino, A.; Griffon, M.; Gagnolet, P.

    1981-03-01

    The report describes a set of categories for reporting industrial incidents and events involving human malfunction. The classification system aims at ensuring information adequate for improvement of human work situations and man-machine interface systems and for attempts to quantify ''human error'' rates. The classification system has a multifacetted non-hierarchial structure and its compatibility with Ispra's ERDS classification is described. The collection of the information in general and for quantification purposes are discussed. 24 categories, 12 of which being human factors oriented, are listed with their respective subcategories, and comments are given. Underlying models of human data processes and their typical malfunctions and of a human decision sequence are described. (author)

  20. Classification system for reporting events involving human malfunctions

    DEFF Research Database (Denmark)

    Rasmussen, Jens; Pedersen, O.M.; Mancini, G.

    1981-01-01

    The report describes a set of categories for reporting indus-trial incidents and events involving human malfunction. The classification system aims at ensuring information adequate for improvement of human work situations and man-machine interface systems and for attempts to quantify "human error......" rates. The classification system has a multifacetted non-hierarchical struc-ture and its compatibility with Isprals ERDS classification is described. The collection of the information in general and for quantification purposes are discussed. 24 categories, 12 of which being human factors oriented......, are listed with their respective subcategories, and comments are given. Underlying models of human data processes and their typical malfunc-tions and of a human decision sequence are described....

  1. Brayton isotope power system, phase I. Final report

    International Nuclear Information System (INIS)

    1978-01-01

    The Phase I program resulted in the development and ground demonstration of a dynamic power conversion system. The two key contractual objectives of 25% conversion efficiency and 1000 h of endurance testing were successfully met. As a result of the Phase I effort, the BIPS is a viable candidate for further development into a flight system capable of sustained operation in space. It represents the only known dynamic space power system to demonstrate the performance and endurance coupled with the simplicity necessary for reliable operation. This final report follows thirty-five monthly reports. For expediency, it makes liberal use of referenced documents which have been submitted to DOE during the course of the program

  2. Foundational Report Series: Advanced Distribution Management Systems for Grid Modernization

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Jianhui [Argonne National Lab. (ANL), Argonne, IL (United States)

    2015-09-01

    This report describes the application functions for distribution management systems (DMS). The application functions are those surveyed by the IEEE Power and Energy Society’s Task Force on Distribution Management Systems. The description of each DMS application includes functional requirements and the key features and characteristics in current and future deployments, as well as a summary of the major benefits provided by each function to stakeholders — from customers to shareholders. Due consideration is paid to the fact that the realizable benefits of each function may differ by type of utility, whether investor-owned, cooperative, or municipal. This report is sufficient to define the functional requirements of each application for system procurement (request-for-proposal [RFP]) purposes and for developing preliminary high-level use cases for those functions. However, it should not be considered a design document that will enable a vendor or software developer to design and build actual DMS applications.

  3. Design review report for the SY-101 RAPID mitigation system

    International Nuclear Information System (INIS)

    SCHLOSSER, R.L.

    1999-01-01

    This report documents design reviews conducted of the SY-101 Respond And Pump In Days (RAPID) Mitigation System. As part of the SY-101 Surface-Level-Rise Remediation Project, the SY-101 WID Mitigation System will reduce the potential unacceptable consequences of crust growth in Tank 241-SY-101 (SY-101). Projections of the crust growth rate indicate that the waste level in the tank may reach the juncture of the primary and secondary confinement structures of the tank late in 1999. Because of this time constraint, many design activities are being conducted in parallel and design reviews were conducted for system adequacy as well as design implementation throughout the process. Design implementation, as used in this design review report, is the final component selection (e.g., which circuit breaker, valve, or thermocouple) that meets the approved design requirements, system design, and design and procurement specifications. Design implementation includes the necessary analysis, testing, verification, and qualification to demonstrate compliance with the system design and design requirements. Design implementation is outside the scope of this design review. The design activities performed prior to detailed design implementation (i.e., system mission requirements, functional design requirements, technical criteria, system conceptual design, and where design and build contracts were placed, the procurement specification) have been reviewed and are within the scope of this design review report. Detailed design implementation will be controlled, reviewed, and where appropriate, approved in accordance with Tank Waste Remediation System (TWRS) engineering procedures. Review of detailed design implementation will continue until all components necessary to perform the transfer function are installed and tested

  4. Design review report for the SY-101 RAPID mitigation system

    Energy Technology Data Exchange (ETDEWEB)

    SCHLOSSER, R.L.

    1999-05-24

    This report documents design reviews conducted of the SY-101 Respond And Pump In Days (RAPID) Mitigation System. As part of the SY-101 Surface-Level-Rise Remediation Project, the SY-101 WID Mitigation System will reduce the potential unacceptable consequences of crust growth in Tank 241-SY-101 (SY-101). Projections of the crust growth rate indicate that the waste level in the tank may reach the juncture of the primary and secondary confinement structures of the tank late in 1999. Because of this time constraint, many design activities are being conducted in parallel and design reviews were conducted for system adequacy as well as design implementation throughout the process. Design implementation, as used in this design review report, is the final component selection (e.g., which circuit breaker, valve, or thermocouple) that meets the approved design requirements, system design, and design and procurement specifications. Design implementation includes the necessary analysis, testing, verification, and qualification to demonstrate compliance with the system design and design requirements. Design implementation is outside the scope of this design review. The design activities performed prior to detailed design implementation (i.e., system mission requirements, functional design requirements, technical criteria, system conceptual design, and where design and build contracts were placed, the procurement specification) have been reviewed and are within the scope of this design review report. Detailed design implementation will be controlled, reviewed, and where appropriate, approved in accordance with Tank Waste Remediation System (TWRS) engineering procedures. Review of detailed design implementation will continue until all components necessary to perform the transfer function are installed and tested.

  5. Preoperational test report, primary ventilation condenser cooling system

    Energy Technology Data Exchange (ETDEWEB)

    Clifton, F.T.

    1997-10-29

    This represents the preoperational test report for the Primary Ventilation Condenser Cooling System, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system uses a closed chilled water piping loop to provide offgas effluent cooling for tanks AY101, AY102, AZ1O1, AZ102; the offgas is cooled from a nominal 100 F to 40 F. Resulting condensation removes tritiated vapor from the exhaust stack stream. The piping system includes a package outdoor air-cooled water chiller with parallel redundant circulating pumps; the condenser coil is located inside a shielded ventilation equipment cell. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System.

  6. Preoperational test report, primary ventilation condenser cooling system

    International Nuclear Information System (INIS)

    Clifton, F.T.

    1997-01-01

    This represents the preoperational test report for the Primary Ventilation Condenser Cooling System, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system uses a closed chilled water piping loop to provide offgas effluent cooling for tanks AY101, AY102, AZ1O1, AZ102; the offgas is cooled from a nominal 100 F to 40 F. Resulting condensation removes tritiated vapor from the exhaust stack stream. The piping system includes a package outdoor air-cooled water chiller with parallel redundant circulating pumps; the condenser coil is located inside a shielded ventilation equipment cell. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System

  7. Auxin efflux by PIN-FORMED proteins is activated by two different protein kinases, D6 PROTEIN KINASE and PINOID

    KAUST Repository

    Zourelidou, Melina; Absmanner, Birgit; Weller, Benjamin; Barbosa, Inê s CR; Willige, Bjö rn C; Fastner, Astrid; Streit, Verena; Port, Sarah A; Colcombet, Jean; de la Fuente van Bentem, Sergio; Hirt, Heribert; Kuster, Bernhard; Schulze, Waltraud X; Hammes, Ulrich Z; Schwechheimer, Claus

    2014-01-01

    The development and morphology of vascular plants is critically determined by synthesis and proper distribution of the phytohormone auxin. The directed cell-to-cell distribution of auxin is achieved through a system of auxin influx and efflux transporters. PIN-FORMED (PIN) proteins are proposed auxin efflux transporters, and auxin fluxes can seemingly be predicted based on the-in many cells-asymmetric plasma membrane distribution of PINs. Here, we show in a heterologous Xenopus oocyte system as well as in Arabidopsis thaliana inflorescence stems that PIN-mediated auxin transport is directly activated by D6 PROTEIN KINASE (D6PK) and PINOID (PID)/WAG kinases of the Arabidopsis AGCVIII kinase family. At the same time, we reveal that D6PKs and PID have differential phosphosite preferences. Our study suggests that PIN activation by protein kinases is a crucial component of auxin transport control that must be taken into account to understand auxin distribution within the plant.

  8. Auxin efflux by PIN-FORMED proteins is activated by two different protein kinases, D6 PROTEIN KINASE and PINOID

    KAUST Repository

    Zourelidou, Melina

    2014-06-19

    The development and morphology of vascular plants is critically determined by synthesis and proper distribution of the phytohormone auxin. The directed cell-to-cell distribution of auxin is achieved through a system of auxin influx and efflux transporters. PIN-FORMED (PIN) proteins are proposed auxin efflux transporters, and auxin fluxes can seemingly be predicted based on the-in many cells-asymmetric plasma membrane distribution of PINs. Here, we show in a heterologous Xenopus oocyte system as well as in Arabidopsis thaliana inflorescence stems that PIN-mediated auxin transport is directly activated by D6 PROTEIN KINASE (D6PK) and PINOID (PID)/WAG kinases of the Arabidopsis AGCVIII kinase family. At the same time, we reveal that D6PKs and PID have differential phosphosite preferences. Our study suggests that PIN activation by protein kinases is a crucial component of auxin transport control that must be taken into account to understand auxin distribution within the plant.

  9. Profiling bacterial kinase activity using a genetic circuit

    DEFF Research Database (Denmark)

    van der Helm, Eric; Bech, Rasmus; Lehning, Christina Eva

    Phosphorylation is a post-translational modification that regulates the activity of several key proteins in bacteria and eukaryotes. Accordingly, a variety of tools has been developed to measure kinase activity. To couple phosphorylation to an in vivo fluorescent readout we used the Bacillus...... subtilis kinase PtkA, transmembrane activator TkmA and the repressor FatR to construct a genetic circuit in E. coli. By tuning the repressor and kinase expression level at the same time, we were able to show a 4.2-fold increase in signal upon kinase induction. We furthermore validated that the previously...... reported FatR Y45E mutation1 attenuates operator repression. This genetic circuit provides a starting point for computational protein design and a metagenomic library-screening tool....

  10. Derrame pericárdico con inminente taponamiento cardiaco secundario a inhibidor de la tirosina quinasa: Reporte de un caso Pericardial effusion secondary to tyrosine kinase inhibitor with imminence of cardiac tamponade: case report

    Directory of Open Access Journals (Sweden)

    Andrés F Buitrago

    2011-04-01

    Full Text Available La leucemia es una enfermedad maligna que se caracteriza por una proliferación no controlada de una clona iniciada en una etapa precoz de la diferenciación linfoide. Es importante determinar si hay una alteración genética conocida como cromosoma Filadelfia, para pronóstico y tratamiento. El imatinib, un inhibidor de la tirosina kinasa, tiene buena respuesta terapéutica y pocos efectos adversos. Uno de frecuente aparición es la serositis manifestada como derrame pleural, aunque es de menor incidencia asociada con derrame pericárdico. El tratamiento se debe individualizar, pero en caso de inminente taponamiento cardiaco se realiza pericardiocentesis.Leukemia is a malignant disease characterized by uncontrolled proliferation of a clone initiated at an early stage of lymphoid differentiation. It is important to determine if there is a genetic disorder known as the Philadelphia chromosome, for both prognosis and treatment. Imatinib, a tyrosine kinase inhibitor has good therapeutic response and few adverse effects. An adverse event is the frequent occurrence of serositis manifested as pleural effusion, but its association with pericardial effusion has a lower incidence. Treatment should be individualized, but in case of imminent cardiac tamponade pericardiocentesis is performed.

  11. Technical assistance contractor occurrence reporting and processing system

    International Nuclear Information System (INIS)

    1996-08-01

    Members of the Uranium Mill Tailings Remedial Action (UMTRA) Project Technical Assistance Contractor (TAC) are responsible to notify management of TAC occurrence reporting and processing system (ORPS) classified occurrences .An ORPS occurrence is an unexpected or unplanned event on DOE property which causes bodily harm, death, damage to government property, exposure to toxic or hazardous substances above acceptable limits to workers, the environment, or general public. Examples of potential reportable occurrences include, but not limited to, site personnel exposures to airborne contaminants, incidents which could expose the general public to high levels of radiation or other contaminants, a vehicle accident resulting in property damage or personnel injuries. Listed TAC manager/staff contacts, with the assistance of TAC ORPS Program Coordinators, will determine if the occurrence is reportable under Department of Energy (DOE) Order M 232.1-2. The reportable occurrences will be classified as emergency, unusual, or off-normal. If determined to be reportable, listed TAC manager/staff will verbally report the details of the occurrence to the DOE Duty Officer within 2 hours of initial notification, and provide a written report of the event by noon the following work day

  12. Protein Kinase A in Cancer

    International Nuclear Information System (INIS)

    Caretta, Antonio; Mucignat-Caretta, Carla

    2011-01-01

    In the past, many chromosomal and genetic alterations have been examined as possible causes of cancer. However, some tumors do not display a clear molecular and/or genetic signature. Therefore, other cellular processes may be involved in carcinogenesis. Genetic alterations of proteins involved in signal transduction have been extensively studied, for example oncogenes, while modifications in intracellular compartmentalization of these molecules, or changes in the expression of unmodified genes have received less attention. Yet, epigenetic modulation of second messenger systems can deeply modify cellular functioning and in the end may cause instability of many processes, including cell mitosis. It is important to understand the functional meaning of modifications in second messenger intracellular pathways and unravel the role of downstream proteins in the initiation and growth of tumors. Within this framework, the cAMP system has been examined. cAMP is a second messenger involved in regulation of a variety of cellular functions. It acts mainly through its binding to cAMP-activated protein kinases (PKA), that were suggested to participate in the onset and progression of various tumors. PKA may represent a biomarker for tumor detection, identification and staging, and may be a potential target for pharmacological treatment of tumors

  13. Protein Kinase A in Cancer

    Energy Technology Data Exchange (ETDEWEB)

    Caretta, Antonio; Mucignat-Caretta, Carla, E-mail: carla.mucignat@unipd.it [Department of Human Anatomy and Physiology, University of Padova, Via Marzolo 3, 35131 Padova (Italy)

    2011-02-28

    In the past, many chromosomal and genetic alterations have been examined as possible causes of cancer. However, some tumors do not display a clear molecular and/or genetic signature. Therefore, other cellular processes may be involved in carcinogenesis. Genetic alterations of proteins involved in signal transduction have been extensively studied, for example oncogenes, while modifications in intracellular compartmentalization of these molecules, or changes in the expression of unmodified genes have received less attention. Yet, epigenetic modulation of second messenger systems can deeply modify cellular functioning and in the end may cause instability of many processes, including cell mitosis. It is important to understand the functional meaning of modifications in second messenger intracellular pathways and unravel the role of downstream proteins in the initiation and growth of tumors. Within this framework, the cAMP system has been examined. cAMP is a second messenger involved in regulation of a variety of cellular functions. It acts mainly through its binding to cAMP-activated protein kinases (PKA), that were suggested to participate in the onset and progression of various tumors. PKA may represent a biomarker for tumor detection, identification and staging, and may be a potential target for pharmacological treatment of tumors.

  14. Quantitative and Dynamic Imaging of ATM Kinase Activity.

    Science.gov (United States)

    Nyati, Shyam; Young, Grant; Ross, Brian Dale; Rehemtulla, Alnawaz

    2017-01-01

    Ataxia telangiectasia mutated (ATM) is a serine/threonine kinase critical to the cellular DNA-damage response, including DNA double-strand breaks (DSBs). ATM activation results in the initiation of a complex cascade of events facilitating DNA damage repair, cell cycle checkpoint control, and survival. Traditionally, protein kinases have been analyzed in vitro using biochemical methods (kinase assays using purified proteins or immunological assays) requiring a large number of cells and cell lysis. Genetically encoded biosensors based on optical molecular imaging such as fluorescence or bioluminescence have been developed to enable interrogation of kinase activities in live cells with a high signal to background. We have genetically engineered a hybrid protein whose bioluminescent activity is dependent on the ATM-mediated phosphorylation of a substrate. The engineered protein consists of the split luciferase-based protein complementation pair with a CHK2 (a substrate for ATM kinase activity) target sequence and a phospho-serine/threonine-binding domain, FHA2, derived from yeast Rad53. Phosphorylation of the serine residue within the target sequence by ATM would lead to its interaction with the phospho-serine-binding domain, thereby preventing complementation of the split luciferase pair and loss of reporter activity. Bioluminescence imaging of reporter expressing cells in cultured plates or as mouse xenografts provides a quantitative surrogate for ATM kinase activity and therefore the cellular DNA damage response in a noninvasive, dynamic fashion.

  15. Operational test report - Project W-320 cathodic protection systems

    International Nuclear Information System (INIS)

    Bowman, T.J.

    1998-01-01

    Washington Administrative Code (WAC) 173-303-640 specifies that corrosion protection must be designed into tank systems that treat or store dangerous wastes. Project W-320, Waste Retrieval Sluicing System (WRSS), utilizes underground encased waste transfer piping between tanks 241-C-106 and 241-AY-102. Corrosion protection is afforded to the encasements of the WRSS waste transfer piping through the application of earthen ionic currents onto the surface of the piping encasements. Cathodic protection is used in conjunction with the protective coatings that are applied upon the WRSS encasement piping. WRSS installed two new two rectifier systems (46 and 47) and modified one rectifier system (31). WAC 173-303-640 specifies that the proper operation of cathodic protection systems must be confirmed within six months after initial installation. The WRSS cathodic protection systems were energized to begin continuous operation on 5/5/98. Sixteen days after the initial steady-state start-up of the WRSS rectifier systems, the operational testing was accomplished with procedure OTP-320-006 Rev/Mod A-0. This operational test report documents the OTP-320-006 results and documents the results of configuration testing of integrated piping and rectifier systems associated with the W-320 cathodic protection systems

  16. WhiteCap System, structural analysis reports. Progress report, April 1995--June 1995

    Energy Technology Data Exchange (ETDEWEB)

    Chai, Y.H.; Romstad, K.M. [Univ. of California, Davis, CA (United States)

    1995-04-27

    The authors have completed an engineering investigation of the CoolRoof System as applicable to typical building construction in the Sacramento Valley. The purpose of this investigation is to provide structural engineering recommendations regarding structural modifications to typical building construction required by CoolRoof. This report presents the results of our investigation.

  17. BI Reporting, Data Warehouse Systems, and Beyond. CDS Spotlight Report. Research Bulletin

    Science.gov (United States)

    Lang, Leah; Pirani, Judith A.

    2014-01-01

    This Spotlight focuses on data from the 2013 Core Data Service [CDS] to better understand how higher education institutions approach business intelligence (BI) reporting and data warehouse systems (see the Sidebar for definitions). Information provided for this Spotlight was derived from Module 8 of CDS, which contains several questions regarding…

  18. E-Business Reporting: Towards a Global Standard for Financial Reporting Systems Using XBRL

    Science.gov (United States)

    Long, Margaret J.

    2013-01-01

    Reporting systems can provide transparency into financial markets necessary for a sustainable, prosperous global economy. The most widely used global platform for exchanging electronic information about companies to regulatory bodies is XBRL. Standards for this platform are in the process of becoming legally harmonized, but not all countries are…

  19. Technical Evaluation Report 14: Chat and Instant Messaging Systems (synchronous: Report 2/ 3

    Directory of Open Access Journals (Sweden)

    Ellen Kinsel

    2002-10-01

    Full Text Available This study extends the earlier review of online chat systems (Report 6, by reviewing a further seven products that provide text-based instant messaging (IM. The functionality of these products in distance education contexts is stressed in relation to their comparative costs, complexity, control, clarity, technical framework, and other functional options.

  20. High level nuclear waste repository in salt: Sealing systems status and planning report: Draft report

    International Nuclear Information System (INIS)

    1985-09-01

    This report documents the initial conceptual design studies for a repository sealing system for a high-level nuclear waste repository in salt. The first step in the initial design studies was to review the current design level, termed schematic designs. This review identified practicality of construction and development of a design methodology as two key issues for the conceptual design. These two issues were then investigated during the initial design studies for seal system materials, seal placement, backfill emplacement, and a testing and monitoring plan. The results of these studies have been used to develop a program plan for completion of the sealing system conceptual design. 60 refs., 26 figs., 18 tabs

  1. Communications and control for electric power systems: Final report

    Energy Technology Data Exchange (ETDEWEB)

    Kirkham, H.

    1998-04-01

    This report is a summary of some of the work done on the Communications and Control project, with particular emphasis on the achievements during the years 1986--1996. During those years, the project moved away from concern with dispersed storage and generation and its impact on power system operation (the team was responsible for studies in this area, and for making a power system simulator that included DSG), and became involved in more concrete work aimed at applying high-tech solutions to problems of power system communications and control. This report covers work done at JPL on the following topics: (1) the measurement of electric and magnetic fields, both ac and dc; (2) the use of optical power to supply low-power electronics; (3) the design of a fault-tolerant communication system designed for distribution automation; and (4) a digital phase locked loop that allows the use of low-power transmitting electronics to recreate a good-quality signal at the receiver. In a report of this kind, only the results and highlights of the work are described.

  2. Src family kinases in chronic kidney disease.

    Science.gov (United States)

    Wang, Jun; Zhuang, Shougang

    2017-09-01

    Src family kinases (SFKs) belong to nonreceptor protein tyrosine kinases and have been implicated in the regulation of numerous cellular processes, including cell proliferation, differentiation, migration and invasion, and angiogenesis. The role and mechanisms of SFKs in tumorgenesis have been extensively investigated, and some SFK inhibitors are currently under clinical trials for tumor treatment. Recent studies have also demonstrated the importance of SFKs in regulating the development of various fibrosis-related chronic diseases (e.g., idiopathic pulmonary fibrosis, liver fibrosis, renal fibrosis, and systemic sclerosis). In this article, we summarize the roles of SFKs in various chronic kidney diseases, including glomerulonephritis, diabetic nephropathy, human immunodeficiency virus-associated nephropathy, autosomal dominant form of polycystic kidney disease, and obesity-associated kidney disease, and discuss the mechanisms involved. Copyright © 2017 the American Physiological Society.

  3. Phosphatidylinositol 4-kinases: Function, structure, and inhibition

    International Nuclear Information System (INIS)

    Boura, Evzen; Nencka, Radim

    2015-01-01

    The phosphatidylinositol 4-kinases (PI4Ks) synthesize phosphatidylinositol 4-phosphate (PI4P), a key member of the phosphoinositide family. PI4P defines the membranes of Golgi and trans-Golgi network (TGN) and regulates trafficking to and from the Golgi. Humans have two type II PI4Ks (α and β) and two type III enzymes (α and β). Recently, the crystal structures were solved for both type II and type III kinase revealing atomic details of their function. Importantly, the type III PI4Ks are hijacked by +RNA viruses to create so-called membranous web, an extensively phosphorylated and modified membrane system dedicated to their replication. Therefore, selective and potent inhibitors of PI4Ks have been developed as potential antiviral agents. Here we focus on the structure and function of PI4Ks and their potential in human medicine

  4. Phosphatidylinositol 4-kinases: Function, structure, and inhibition

    Energy Technology Data Exchange (ETDEWEB)

    Boura, Evzen, E-mail: boura@uochb.cas.cz; Nencka, Radim, E-mail: nencka@uochb.cas.cz

    2015-10-01

    The phosphatidylinositol 4-kinases (PI4Ks) synthesize phosphatidylinositol 4-phosphate (PI4P), a key member of the phosphoinositide family. PI4P defines the membranes of Golgi and trans-Golgi network (TGN) and regulates trafficking to and from the Golgi. Humans have two type II PI4Ks (α and β) and two type III enzymes (α and β). Recently, the crystal structures were solved for both type II and type III kinase revealing atomic details of their function. Importantly, the type III PI4Ks are hijacked by +RNA viruses to create so-called membranous web, an extensively phosphorylated and modified membrane system dedicated to their replication. Therefore, selective and potent inhibitors of PI4Ks have been developed as potential antiviral agents. Here we focus on the structure and function of PI4Ks and their potential in human medicine.

  5. Risoe energy report 7. Future low carbon energy systems

    Energy Technology Data Exchange (ETDEWEB)

    Larsen, Hans; Soenderberg Petersen, L. (eds.)

    2008-10-15

    This Risoe Energy Report, the seventh of a series that began in 2002, takes as its point of reference the recommendations of the Intergovernmental Panel on Climate Change (IPCC) in 2007. The IPCC states that if anticipated climate change is to remain in the order of 2 to 3 degrees centigrades over the next century, the world's CO{sub 2} emissions would have to peak within the next 10-15 years and ultimately be reduced to approximately 50% of their present level by the middle of the century. The IPCC states further that this would be possible, provided that serious action is taken now. The different regions and countries of the world are in various states of development, and hence have different starting points for contributing to these reductions in CO{sub 2} emissions. This report presents state-of-the-art and development perspectives for energy supply technologies, new energy systems, end-use energy efficiency improvements and new policy measures. It also includes estimates of the CO{sub 2} reduction potentials for different technologies. The technologies are characterized with regard to their ability to contribute either to ensuring a peak in CO{sub 2} emissions within 10-15 years, or to long-term CO{sub 2} reductions. The report outlines the current and likely future composition of energy systems in Denmark, and examines three groups of countries: i) Europe and the other OECD member nations; ii) large and rapidly growing developing economies, notably India and China; iii) typical least developed countries, such as many African nations. The report emphasises how future energy developments and systems might be composed in these three country groupings, and to what extent the different technologies might contribute. The report addresses the need for research and demonstration together with market incentives, and policy measures with focus on initiatives that can promote the development towards CO{sub 2} reductions. Specifically, the report identifies system

  6. Dynamic Systems Analysis Report for Nuclear Fuel Recycle

    Energy Technology Data Exchange (ETDEWEB)

    Brent Dixon; Sonny Kim; David Shropshire; Steven Piet; Gretchen Matthern; Bill Halsey

    2008-12-01

    This report examines the time-dependent dynamics of transitioning from the current United States (U.S.) nuclear fuel cycle where used nuclear fuel is disposed in a repository to a closed fuel cycle where the used fuel is recycled and only fission products and waste are disposed. The report is intended to help inform policy developers, decision makers, and program managers of system-level options and constraints as they guide the formulation and implementation of advanced fuel cycle development and demonstration efforts and move toward deployment of nuclear fuel recycling infrastructure.

  7. Building America Systems Integration Research Annual Report: FY 2012

    Energy Technology Data Exchange (ETDEWEB)

    Gestwick, M.

    2013-05-01

    This document is the Building America FY2012 Annual Report, which includes an overview of the Building America Program activities and the work completed by the National Renewable Energy Laboratory and the Building America industry consortia (the Building America teams). The annual report summarizes major technical accomplishments and progress towards U.S. Department of Energy Building Technologies Program's multi-year goal of developing the systems innovations that enable risk-free, cost effective, reliable and durable efficiency solutions that reduce energy use by 30%-50% in both new and existing homes.

  8. Building America Systems Integration Research Annual Report. FY 2012

    Energy Technology Data Exchange (ETDEWEB)

    Gestwick, Michael [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2013-05-01

    This Building America FY2012 Annual Report includes an overview of the Building America Program activities and the work completed by the National Renewable Energy Laboratory and the Building America industry consortia (the Building America teams). The annual report summarizes major technical accomplishments and progress towards U.S. Department of Energy Building Technologies Program's multi-year goal of developing the systems innovations that enable risk-free, cost effective, reliable and durable efficiency solutions that reduce energy use by 30%-50% in both new and existing homes.

  9. Breast imaging and reporting data system (BIRADS): Magnetic resonance imaging

    International Nuclear Information System (INIS)

    Tardivon, Anne A.; Athanasiou, Alexandra; Thibault, Fabienne; El Khoury, Carl

    2007-01-01

    This article reviews the technical aspects and interpretation criteria in breast MR imaging based on the first edition of breast imaging and reporting data system (BIRADS) published by the American College of Radiology (ACR) in 2003. In a second article, practical cases will be proposed for training the readers. The major aims of using this lexicon are: first to use a logical and standardized description of MR lesions, secondly to obtain a structured MR report with a clear final impression (BIRADS assessment categories), and thirdly to help comparison between different clinical studies based on similar breast MRI terminology

  10. A conserved cysteine motif is critical for rice ceramide kinase activity and function.

    Directory of Open Access Journals (Sweden)

    Fang-Cheng Bi

    Full Text Available Ceramide kinase (CERK is a key regulator of cell survival in dicotyledonous plants and animals. Much less is known about the roles of CERK and ceramides in mediating cellular processes in monocot plants. Here, we report the characterization of a ceramide kinase, OsCERK, from rice (Oryza sativa spp. Japonica cv. Nipponbare and investigate the effects of ceramides on rice cell viability.OsCERK can complement the Arabidopsis CERK mutant acd5. Recombinant OsCERK has ceramide kinase activity with Michaelis-Menten kinetics and optimal activity at 7.0 pH and 40°C. Mg2+ activates OsCERK in a concentration-dependent manner. Importantly, a CXXXCXXC motif, conserved in all ceramide kinases and important for the activity of the human enzyme, is critical for OsCERK enzyme activity and in planta function. In a rice protoplast system, inhibition of CERK leads to cell death and the ratio of added ceramide and ceramide-1-phosphate, CERK's substrate and product, respectively, influences cell survival. Ceramide-induced rice cell death has apoptotic features and is an active process that requires both de novo protein synthesis and phosphorylation, respectively. Finally, mitochondria membrane potential loss previously associated with ceramide-induced cell death in Arabidopsis was also found in rice, but it occurred with different timing.OsCERK is a bona fide ceramide kinase with a functionally and evolutionarily conserved Cys-rich motif that plays an important role in modulating cell fate in plants. The vital function of the conserved motif in both human and rice CERKs suggests that the biochemical mechanism of CERKs is similar in animals and plants. Furthermore, ceramides induce cell death with similar features in monocot and dicot plants.

  11. Structural Insights into the HWE Histidine Kinase Family: The Brucella Blue Light-Activated Histidine Kinase Domain.

    Science.gov (United States)

    Rinaldi, Jimena; Arrar, Mehrnoosh; Sycz, Gabriela; Cerutti, María Laura; Berguer, Paula M; Paris, Gastón; Estrín, Darío Ariel; Martí, Marcelo Adrián; Klinke, Sebastián; Goldbaum, Fernando Alberto

    2016-03-27

    In response to light, as part of a two-component system, the Brucella blue light-activated histidine kinase (LOV-HK) increases its autophosphorylation, modulating the virulence of this microorganism. The Brucella histidine kinase (HK) domain belongs to the HWE family, for which there is no structural information. The HWE family is exclusively present in proteobacteria and usually coupled to a wide diversity of light sensor domains. This work reports the crystal structure of the Brucella HK domain, which presents two different dimeric assemblies in the asymmetric unit: one similar to the already described canonical parallel homodimers (C) and the other, an antiparallel non-canonical (NC) dimer, each with distinct relative subdomain orientations and dimerization interfaces. Contrary to these crystallographic structures and unlike other HKs, in solution, the Brucella HK domain is monomeric and still active, showing an astonishing instability of the dimeric interface. Despite this instability, using cross-linking experiments, we show that the C dimer is the functionally relevant species. Mutational analysis demonstrates that the autophosphorylation activity occurs in cis. The different relative subdomain orientations observed for the NC and C states highlight the large conformational flexibility of the HK domain. Through the analysis of these alternative conformations by means of molecular dynamics simulations, we also propose a catalytic mechanism for Brucella LOV-HK. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. An Evaluation of Departmental Radiation Oncology Incident Reports: Anticipating a National Reporting System

    International Nuclear Information System (INIS)

    Terezakis, Stephanie A.; Harris, Kendra M.; Ford, Eric; Michalski, Jeff; DeWeese, Theodore; Santanam, Lakshmi; Mutic, Sasa; Gay, Hiram

    2013-01-01

    Purpose: Systems to ensure patient safety are of critical importance. The electronic incident reporting systems (IRS) of 2 large academic radiation oncology departments were evaluated for events that may be suitable for submission to a national reporting system (NRS). Methods and Materials: All events recorded in the combined IRS were evaluated from 2007 through 2010. Incidents were graded for potential severity using the validated French Nuclear Safety Authority (ASN) 5-point scale. These incidents were categorized into 7 groups: (1) human error, (2) software error, (3) hardware error, (4) error in communication between 2 humans, (5) error at the human-software interface, (6) error at the software-hardware interface, and (7) error at the human-hardware interface. Results: Between the 2 systems, 4407 incidents were reported. Of these events, 1507 (34%) were considered to have the potential for clinical consequences. Of these 1507 events, 149 (10%) were rated as having a potential severity of ≥2. Of these 149 events, the committee determined that 79 (53%) of these events would be submittable to a NRS of which the majority was related to human error or to the human-software interface. Conclusions: A significant number of incidents were identified in this analysis. The majority of events in this study were related to human error and to the human-software interface, further supporting the need for a NRS to facilitate field-wide learning and system improvement

  13. An Evaluation of Departmental Radiation Oncology Incident Reports: Anticipating a National Reporting System

    Energy Technology Data Exchange (ETDEWEB)

    Terezakis, Stephanie A., E-mail: stereza1@jhmi.edu [Department of Radiation Oncology and Molecular Radiation Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland (United States); Harris, Kendra M. [Department of Radiation Oncology and Molecular Radiation Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland (United States); Ford, Eric [Department of Radiation Oncology and Molecular Radiation Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland (United States); Department of Radiation Oncology, University of Washington, Seattle, Washington (United States); Michalski, Jeff [Department of Radiation Oncology, Washington University School of Medicine, St. Louis, Missouri (United States); DeWeese, Theodore [Department of Radiation Oncology and Molecular Radiation Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland (United States); Santanam, Lakshmi; Mutic, Sasa; Gay, Hiram [Department of Radiation Oncology, Washington University School of Medicine, St. Louis, Missouri (United States)

    2013-03-15

    Purpose: Systems to ensure patient safety are of critical importance. The electronic incident reporting systems (IRS) of 2 large academic radiation oncology departments were evaluated for events that may be suitable for submission to a national reporting system (NRS). Methods and Materials: All events recorded in the combined IRS were evaluated from 2007 through 2010. Incidents were graded for potential severity using the validated French Nuclear Safety Authority (ASN) 5-point scale. These incidents were categorized into 7 groups: (1) human error, (2) software error, (3) hardware error, (4) error in communication between 2 humans, (5) error at the human-software interface, (6) error at the software-hardware interface, and (7) error at the human-hardware interface. Results: Between the 2 systems, 4407 incidents were reported. Of these events, 1507 (34%) were considered to have the potential for clinical consequences. Of these 1507 events, 149 (10%) were rated as having a potential severity of ≥2. Of these 149 events, the committee determined that 79 (53%) of these events would be submittable to a NRS of which the majority was related to human error or to the human-software interface. Conclusions: A significant number of incidents were identified in this analysis. The majority of events in this study were related to human error and to the human-software interface, further supporting the need for a NRS to facilitate field-wide learning and system improvement.

  14. Safety analysis report for packaging (onsite) doorstop samplecarrier system

    Energy Technology Data Exchange (ETDEWEB)

    Obrien, J.H.

    1997-02-24

    The Doorstop Sample Carrier System consists of a Type B certified N-55 overpack, U.S. Department of Transportation (DOT) specification or performance-oriented 208-L (55-gal) drum (DOT 208-L drum), and Doorstop containers. The purpose of the Doorstop Sample Carrier System is to transport samples onsite for characterization. This safety analysis report for packaging (SARP) provides the analyses and evaluation necessary to demonstrate that the Doorstop Sample Carrier System meets the requirements and acceptance criteria for both Hanford Site normal transport conditions and accident condition events for a Type B package. This SARP also establishes operational, acceptance, maintenance, and quality assurance (QA) guidelines to ensure that the method of transport for the Doorstop Sample Carrier System is performed safely in accordance with WHC-CM-2-14, Hazardous Material Packaging and Shipping.

  15. Annual Report 1991. Institute for systems engineering and informatics

    International Nuclear Information System (INIS)

    1992-01-01

    The report presents the achievements of the Institute for Systems Engineering and Informatics (ISEI) of the Joint Research Centre (JRC) of the Commission of the European Communities (CEC) for 1991. The JRC is a European scientific and technical research centre established by the member states of the CEC. Its four sites in Belgium (Geel), Germany (Karlsruhe), the Netherland (Petten) and Italy (Ispra) house 8 institutes, each with its own focus of expertise. ISEI, based at Ispra, was created in 1990 by the merger of the Institute for Systems Engineering (ISE) and the Centre for Information Technologies and Electronics (CITE). The main areas of activity of the Institute are: - Industrial and Environmental Risk, - Nuclear Safeguards, -Fusion Reactor Systems Integration and Safety, - Solar Energy Systems and Energy Management, - Advanced Computing, - Informatic services

  16. wKinMut-2: Identification and Interpretation of Pathogenic Variants in Human Protein Kinases

    DEFF Research Database (Denmark)

    Vazquez, Miguel; Pons, Tirso; Brunak, Søren

    2016-01-01

    forest approach. To understand the biological mechanisms causative of human diseases and cancer, information from pertinent reference knowledgebases and the literature is automatically mined, digested and homogenized. Variants are visualized in their structural contexts and residues affecting catalytic...... is often scattered across different sources, which makes the integrative analysis complex and laborious. wKinMut-2 constitutes a solution to facilitate the interpretation of the consequences of human protein kinase variation. Nine methods predict their pathogenicity, including a kinase-specific random...... and drug-binding are identified. Known protein-protein interactions are reported. Altogether, this information is intended to assist the generation of new working hypothesis to be corroborated with ulterior experimental work. The wKinMut-2 system, along with a user manual and examples is freely accessible...

  17. Engineered Barrier System Degradation, Flow, and Transport Process Model Report

    Energy Technology Data Exchange (ETDEWEB)

    E.L. Hardin

    2000-07-17

    The Engineered Barrier System Degradation, Flow, and Transport Process Model Report (EBS PMR) is one of nine PMRs supporting the Total System Performance Assessment (TSPA) being developed by the Yucca Mountain Project for the Site Recommendation Report (SRR). The EBS PMR summarizes the development and abstraction of models for processes that govern the evolution of conditions within the emplacement drifts of a potential high-level nuclear waste repository at Yucca Mountain, Nye County, Nevada. Details of these individual models are documented in 23 supporting Analysis/Model Reports (AMRs). Nineteen of these AMRs are for process models, and the remaining 4 describe the abstraction of results for application in TSPA. The process models themselves cluster around four major topics: ''Water Distribution and Removal Model, Physical and Chemical Environment Model, Radionuclide Transport Model, and Multiscale Thermohydrologic Model''. One AMR (Engineered Barrier System-Features, Events, and Processes/Degradation Modes Analysis) summarizes the formal screening analysis used to select the Features, Events, and Processes (FEPs) included in TSPA and those excluded from further consideration. Performance of a potential Yucca Mountain high-level radioactive waste repository depends on both the natural barrier system (NBS) and the engineered barrier system (EBS) and on their interactions. Although the waste packages are generally considered as components of the EBS, the EBS as defined in the EBS PMR includes all engineered components outside the waste packages. The principal function of the EBS is to complement the geologic system in limiting the amount of water contacting nuclear waste. A number of alternatives were considered by the Project for different EBS designs that could provide better performance than the design analyzed for the Viability Assessment. The design concept selected was Enhanced Design Alternative II (EDA II).

  18. A Crowdsourcing-based Taiwan Scientific Earthquake Reporting System

    Science.gov (United States)

    Liang, W. T.; Lee, J. C.; Lee, C. F.

    2017-12-01

    To collect immediately field observations for any earthquake-induced ground damages, such as surface fault rupture, landslide, rock fall, liquefaction, and landslide-triggered dam or lake, etc., we are developing an earthquake damage reporting system which particularly relies on school teachers as volunteers after taking a series of training courses organized by this project. This Taiwan Scientific Earthquake Reporting (TSER) system is based on the Ushahidi mapping platform, which has been widely used for crowdsourcing on different purposes. Participants may add an app-like icon for mobile devices to this website at https://ies-tser.iis.sinica.edu.tw. Right after a potential damaging earthquake occurred in the Taiwan area, trained volunteers will be notified/dispatched to the source area to carry out field surveys and to describe the ground damages through this system. If the internet is available, they may also upload some relevant images in the field right away. This collected information will be shared with all public after a quick screen by the on-duty scientists. To prepare for the next strong earthquake, we set up a specific project on TSER for sharing spectacular/remarkable geologic features wherever possible. This is to help volunteers get used to this system and share any teachable material on this platform. This experimental, science-oriented crowdsourcing system was launched early this year. Together with a DYFI-like intensity reporting system, Taiwan Quake-Catcher Network, and some online games and teaching materials, the citizen seismology has been much improved in Taiwan in the last decade. All these constructed products are now either operated or promoted at the Taiwan Earthquake Research Center (TEC). With these newly developed platforms and materials, we are aiming not only to raise the earthquake awareness and preparedness, but also to encourage public participation in earthquake science in Taiwan.

  19. Engineered Barrier System Degradation, Flow, and Transport Process Model Report

    International Nuclear Information System (INIS)

    E.L. Hardin

    2000-01-01

    The Engineered Barrier System Degradation, Flow, and Transport Process Model Report (EBS PMR) is one of nine PMRs supporting the Total System Performance Assessment (TSPA) being developed by the Yucca Mountain Project for the Site Recommendation Report (SRR). The EBS PMR summarizes the development and abstraction of models for processes that govern the evolution of conditions within the emplacement drifts of a potential high-level nuclear waste repository at Yucca Mountain, Nye County, Nevada. Details of these individual models are documented in 23 supporting Analysis/Model Reports (AMRs). Nineteen of these AMRs are for process models, and the remaining 4 describe the abstraction of results for application in TSPA. The process models themselves cluster around four major topics: ''Water Distribution and Removal Model, Physical and Chemical Environment Model, Radionuclide Transport Model, and Multiscale Thermohydrologic Model''. One AMR (Engineered Barrier System-Features, Events, and Processes/Degradation Modes Analysis) summarizes the formal screening analysis used to select the Features, Events, and Processes (FEPs) included in TSPA and those excluded from further consideration. Performance of a potential Yucca Mountain high-level radioactive waste repository depends on both the natural barrier system (NBS) and the engineered barrier system (EBS) and on their interactions. Although the waste packages are generally considered as components of the EBS, the EBS as defined in the EBS PMR includes all engineered components outside the waste packages. The principal function of the EBS is to complement the geologic system in limiting the amount of water contacting nuclear waste. A number of alternatives were considered by the Project for different EBS designs that could provide better performance than the design analyzed for the Viability Assessment. The design concept selected was Enhanced Design Alternative II (EDA II)

  20. Technical study report on reprocessing systems. The report of the feasibility study on commercialized FR cycle systems (phase I)

    International Nuclear Information System (INIS)

    Tanaka, Hiroshi; Kawamura, Fumio; Kakehi, Isao

    2001-04-01

    As a part of the feasibility study (FS) on commercialized fast reactor (FR) cycle systems started on July 1999, the design studies and the technical assessments for various advanced reprocessing systems have been carried out. In this study, plant design for the advanced aqueous system and the three non-aqueous systems (oxide electrowinning method, metal electrorefining method, and fluoride volatility method) has been carried out, and each system has been evaluated mainly from the viewpoint of economics. The future R and D issues on the processes and systems have been also clarified. This report describes the results of the study for two years as final report of FS phase I. (1) The advanced aqueous system, based on the simplified PUREX process, has been shown to be much more economical than the conventional PUREX. The 200 tHM/y plant achieves the target of economics, but the 50 tHM/y plant can not achieve the target. (2) The promising alternative systems replaced for advanced aqueous are the supercritical fluid direct extraction method and amine extraction method from the economical viewpoint. The ion exchange method is promising as the process for minor actinide recovery. (3) For reprocessing MOX fuel, all non-aqueous plants with a capacity of 200 tHM/y achieve the economical target. For such a small capacity as 50 tHM/y, further rationalization of the process is required for the oxide electrowinning method and metal electrorefining method to attain the target, though they are more economical than the advanced aqueous system. (4) For metallic and nitride fuel reprocessing, a metal electrorefining system has been shown to be advantageous. (author)