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Sample records for kangkong ipomoea aquatica

  1. Biochar and Glomus caledonium Influence Cd Accumulation of Upland Kangkong (Ipomoea aquatica Forsk.) Intercropped with Alfred Stonecrop (Sedum alfredii Hance)

    Science.gov (United States)

    Hu, Junli; Wu, Fuyong; Wu, Shengchun; Lam, Cheung Lung; Lin, Xiangui; Wong, Ming Hung

    2014-01-01

    Both biochar application and mycorrhizal inoculation have been proposed to improve plant growth and alter bioaccumulation of toxic metals. A greenhouse pot trial was conducted to investigate growth and Cd accumulation of upland kangkong (Ipomoea aquatica Forsk.) intercropped with Alfred stonecrop (Sedum alfredii Hance) in a Cd-contaminated soil inoculated with Glomus caledonium and/or applied with biochar. Compared with the monocultural control, intercropping with stonecrop (IS) decreased kangkong Cd acquisition via rhizosphere competition, and also decreased kangkong yield. Gc inoculation (+M) accelerated growth and Cd acquisition of stonecrop, and hence resulted in further decreases in kangkong Cd acquisition. Regardless of IS and +M, biochar addition (+B) increased kangkong yield via elevating soil available P, and decreased soil Cd phytoavailability and kangkong Cd concentration via increasing soil pH. Compared with the control, the treatment of IS + M + B had a substantially higher kangkong yield (+25.5%) with a lower Cd concentration (−62.7%). Gc generated additive effects on soil alkalinization and Cd stabilization to biochar, causing lower DTPA-extractable (phytoavailable) Cd concentrations and post-harvest transfer risks. PMID:24728157

  2. Biochar and Glomus caledonium Influence Cd Accumulation of Upland Kangkong (Ipomoea aquatica Forsk.) Intercropped with Alfred Stonecrop (Sedum alfredii Hance)

    Science.gov (United States)

    Hu, Junli; Wu, Fuyong; Wu, Shengchun; Lam, Cheung Lung; Lin, Xiangui; Wong, Ming Hung

    2014-04-01

    Both biochar application and mycorrhizal inoculation have been proposed to improve plant growth and alter bioaccumulation of toxic metals. A greenhouse pot trial was conducted to investigate growth and Cd accumulation of upland kangkong (Ipomoea aquatica Forsk.) intercropped with Alfred stonecrop (Sedum alfredii Hance) in a Cd-contaminated soil inoculated with Glomus caledonium and/or applied with biochar. Compared with the monocultural control, intercropping with stonecrop (IS) decreased kangkong Cd acquisition via rhizosphere competition, and also decreased kangkong yield. Gc inoculation (+M) accelerated growth and Cd acquisition of stonecrop, and hence resulted in further decreases in kangkong Cd acquisition. Regardless of IS and +M, biochar addition (+B) increased kangkong yield via elevating soil available P, and decreased soil Cd phytoavailability and kangkong Cd concentration via increasing soil pH. Compared with the control, the treatment of IS + M + B had a substantially higher kangkong yield (+25.5%) with a lower Cd concentration (-62.7%). Gc generated additive effects on soil alkalinization and Cd stabilization to biochar, causing lower DTPA-extractable (phytoavailable) Cd concentrations and post-harvest transfer risks.

  3. Biochar and Glomus caledonium Influence Cd Accumulation of Upland Kangkong (Ipomoea aquatica Forsk.) Intercropped with Alfred Stonecrop (Sedum alfredii Hance)

    OpenAIRE

    Junli Hu; Fuyong Wu; Shengchun Wu; Cheung Lung Lam; Xiangui Lin; Ming Hung Wong

    2014-01-01

    Both biochar application and mycorrhizal inoculation have been proposed to improve plant growth and alter bioaccumulation of toxic metals. A greenhouse pot trial was conducted to investigate growth and Cd accumulation of upland kangkong (Ipomoea aquatica Forsk.) intercropped with Alfred stonecrop (Sedum alfredii Hance) in a Cd-contaminated soil inoculated with Glomus caledonium and/or applied with biochar. Compared with the monocultural control, intercropping with stonecrop (IS) decreased kan...

  4. Arbuscular mycorrhizal fungi induced differential Cd and P phytoavailability via intercropping of upland kangkong (Ipomoea aquatica Forsk.) with Alfred stonecrop (Sedum alfredii Hance): post-harvest study.

    Science.gov (United States)

    Hu, Junli; Li, Jintian; Wu, Fuyong; Wu, Shengchun; Ye, Zhihong; Lin, Xiangui; Wong, Ming Hung

    2013-12-01

    A post-harvest experiment was conducted further to our previous greenhouse pot study on upland kangkong (Ipomoea aquatica Forsk.) and Alfred stonecrop (Sedum alfredii Hance) intercropping system in Cd-contaminated soil inoculated with arbuscular mycorrhizal (AM) fungi. Previously, four treatments were established in the intercropping experiment, including monoculture of kangkong (control), intercropping with stonecrop (IS), and IS plus inoculation with Glomus caledonium (IS+Gc) or Glomus versiforme (IS+Gv). Both kangkong and stonecrop plants were harvested after growing for 8 weeks. Then, the tested soils were reclaimed for growing post-harvest kangkong for 6 weeks. In the post-harvest experiment, there were no significant differences between the IS and control treatments, except for a significantly decreased (pkangkong. In addition, both Gc and Gv significantly increased (pkangkong yield, while Gc only significantly elevated (p<0.05) the shoot P concentration. It suggested that AM fungi have played key roles in Cd stabilization and P mobilization in the intercropping system, and such positive responses seemed to be sustainable and valuable in post-harvest soils.

  5. Interactions between Ipomoea aquatica and Microbial Populations

    Institute of Scientific and Technical Information of China (English)

    Kan; Yuanqing; Sun; Ling; Zhang; Ying

    2014-01-01

    [Objective]This paper was to research the water purification mechanism of Ipomoea aquatica and its correlation with algae and rotifer. [Methods]Taking I. aquatica as the test material,Chlorella vulgaris,Scenedesmus obliquus,Microcystis aeroginosa and rotifer Adineta vaga with different densities were added to the hydroponics nutrients solutions of I. aquatica by the hydroponic ecological simulation method. The growth characteristics of I. aquatica,changes of microbial populations and the consumption status of nutrients in the nutritional solution were determined. And the interactions between the plant and the microbial populations were researched. [Results]When I. aquatica seedlings grew to a certain stage,growth of principal root stopped; while the lateral roots emerged greatly; and the nutrition absorption efficiency enhanced. As the inoculation concentration of C. vulgaris increased,root length of I. aquatica increased relatively great due to the competition for nutrients. The competition and allelopathy of M. aeroginosa and S. obliquus restricted the development of root system of I. aquatica. The grazing pressure of Chlorella vulgaris had little effects on M. aeroginosa,but restricted the rapid growth of S. obliquus. [Conclusions]This research provided data support for the application of fish-shrimp-vegetable aquaculture system.

  6. Optimum fertilizer rate for kangkong (Ipomoea reptans L.) production in ultisol Jasinga

    OpenAIRE

    Anas D Susila; T. Prasetyo; Kartika, J.G.

    2008-01-01

    Proper fertilization of a crop is knowing the crop nutrient requirement for production of maximum yield and the potential level of nutrient availability from the soil. In addition to yield obtained, quality of the commodity is an important factor of profit and shelf-life for high-value crops. Nitrogen, phosphor, and potassium availability are the most limiting factors for maximum growth and yield. Kangkong (Ipomoea reptans L.) are important traditional leafy vegetables crops cultivated in Ind...

  7. Poisoning of 'binthamburu' (Ipomoea asarifolia) due to misidentification as 'kankun' (Ipomoea aquatica).

    Science.gov (United States)

    Ratnatilaka, A; Yakandawala, D; Rupasinghe, N; Ratnayake, K

    2010-06-01

    Ingestion of 'Binthamburu' (Ipomoea asarifolia) by misidentification as 'kankun' (Ipomoea aquatica) as a leafy vegetable causes acute gastrointestinal symptoms and confusion. The authors have encountered four such cases in the past. All cases have been recorded from the dry zone of the country. Both plants are two trailing vines similar in their appearance and preferring the wet habitats. During the course of the day when exposed to sunlight, 'binthamburu' leaves mimic 'kankun' leaves by folding the leaf margins making it difficult to separate the two during harvest and only a closer examination will reveal the difference in their leaf shapes. Ipomoea asarifolia toxicity in human has not been recorded but animal toxicity in North Brazil due to ingestion of Ipomoea asarifolia had been investigated and linked to a toxic substance identified as lectin or LTS.

  8. Phytoremediation of lead using Ipomoea aquatica Forsk. in hydroponic solution.

    Science.gov (United States)

    Bedabati Chanu, Laitonjam; Gupta, Abhik

    2016-08-01

    Ipomoea aquatica Forsk., an aquatic macrophyte, was assessed for its ability to accumulate lead (Pb) by exposing it to graded concentrations of this metal. Accumulation of Pb was the highest in root followed by that in stem and leaf with translocation factor (TF) values of less than unity. On the other hand, all bioconcentration factor (BCF) values in root, stem and leaf were greater than unity. Furthermore, exposure to Pb concentrations over about 20 mg L(-1) induced colour changes in the basal portion of stem which had significantly higher Pb accumulation than that in the unaffected apical part. This resulted in sequestration of excess metal in affected stem tissue, which could take up Pb by the process of caulofiltration or shoot filtration, and served as a secondary reservoir of Pb in addition to the root. The apical parts contained less lead and could regrow roots from nodes and survive when kept in Pb-free medium. The ability of the plant to store Pb in its root and lower part of stem coupled with its ability to propagate by fragmentation through production of adventitious roots and lateral branches from nodes raises the possibility of utilizing Ipomoea aquatica for Pb phytoremediation from liquid effluent. Copyright © 2016 Elsevier Ltd. All rights reserved.

  9. Nutritional Composition of Water Spinach (Ipomoea aquatica Forsk.) Leaves

    Science.gov (United States)

    Umar, K. J.; Hassan, L. G.; Dangoggo, S. M.; Ladan, M. J.

    Analyses of the nutritional composition of water spinach (Ipomoea aquatica) Forsk leaves were carried out using standard methods of food analysis. The proximate composition as well as mineral elements were determined. The leaves were found on dry weight basis to have high moisture (72.83±0.29%), ash (10.83±0.80%), crude lipid (11.00±0.50%), crude fibre (17.67±0.35%) and available carbohydrate (54.20±0.68%), but low in crude protein content (6.30±0.27%). The leaves also have energy value (300.94±5.31 kcal/100 g) that is within the range reported in some Nigerian leafy vegetables. The mineral element contents were high with remarkable concentration of K (5,458.33±954.70 mg/100 g) and Fe (210.30±2.47 mg/100 g). Also the leaves content moderate concentrations of Na (135.00±2.50 mg/100 g), calcium (416.70±5.77 mg/100 g), Magnesium (301.64±12.69 mg/100 g) and P (109.29±0.55 mg/100 g), with low Cu (0.36±0.01 mg/100 g), Mn (2.14±0.22 mg/100 g) and Zn (2.47±0.27 mg/100 g) contents. Comparing the mineral content with recommended dietary allowance, it was showed that the plant leaves is good sources of K, Mn and Fe for all categories of people, while Mg is adequate enough for adult female and children. From the result, Ipomoea aquatica Forsk leaves could be used for nutritional purposes, due to the amount and diversity of nutrients it contains.

  10. Ipomoea aquatica Extract Shows Protective Action Against Thioacetamide-Induced Hepatotoxicity

    OpenAIRE

    Hadi, A. Hamid A.; Siddig Ibrahim Abdelwahab; Suzy Munir Salama; Salim Said Alkiyumi; Mahmood Ameen Abdullah; Ahmed Salim Alrashdi

    2012-01-01

    In the Indian system of traditional medicine (Ayurveda) it is recommended to consume Ipomoea aquatica to mitigate disorders like jaundice. In this study, the protective effects of ethanol extract of I. aquatica against liver damage were evaluated in thioacetamide (TAA)-induced chronic hepatotoxicity in rats. There was no sign of toxicity in the acute toxicity study, in which Sprague-Dawley (SD) rats were orally fed with ...

  11. Phyto-remediation potential of Ipomoea aquatica for Cr(VI) mitigation.

    Science.gov (United States)

    Weerasinghe, Aruni; Ariyawnasa, Sanduni; Weerasooriya, Rohan

    2008-01-01

    Phyto-remedial efficiency of Ipomoea aquatica was examined at different experimental conditions for a period of 3 months. This plant was selected due to its easy establishment, tolerance and growing easiness. In all trials, the I. aquatica was grown in coir dust to ensure an inert medium. Essential growth nutrients were supplied externally using Albert solution. Once plant growth conditions were fixed, the model system was spiked with Cr(VI) solution in the range of 7-90 ppm. Up to 28 ppm Cr(VI), I. aquatica exhibits uniform absorption characteristics showing over 75% removal of added Cr(VI). At this stage I. aquatica was not affected and it showed no toxicity symptoms. Therefore, it is suited as a potential phyto-remediant. Further I. aquatica is a vegetable particularly in Asian region; therefore caution has to be taken when selecting it for human consumption due to its high chromium accumulation capacity.

  12. "allometry" Deterministic Approaches in Cell Size, Cell Number and Crude Fiber Content Related to the Physical Quality of Kangkong (Ipomoea reptans) Grown Under Different Plant Density Pressures

    Science.gov (United States)

    Selamat, A.; Atiman, S. A.; Puteh, A.; Abdullah, N. A. P.; Mohamed, M. T. M.; Zulkeefli, A. A.; Othman, S.

    Kangkong, especially the upland type (Ipomoea reptans) is popularly consumed as a vegetable dish in the South East Asian countries for its quality related to Vitamins (A and C) and crude fiber contents. Higher fiber contents would prevent from the occurrence of colon cancer and diverticular disease. With young stem edible portion, its cell number and size contribute to the stem crude fiber content. The mathematical approach of allometry of cell size, number, and fiber content of stem could be used in determining the 'best' plant density pressure in producing the quality young stem to be consumed. Basically, allometry is the ratio of relative increment (growth or change) rates of two parameters, or the change rate associated to the log of measured variables relationship. Kangkog grown equal or lower than 55 plants m-2 produced bigger individual plant and good quality (physical) kangkong leafy vegetable, but with lower total yield per unit area as compared to those grown at higher densities.

  13. Ipomoea hederifolia rooted soil bed and Ipomoea aquatica rhizofiltration coupled phytoreactors for efficient treatment of textile wastewater.

    Science.gov (United States)

    Rane, Niraj R; Patil, Swapnil M; Chandanshive, Vishal V; Kadam, Suhas K; Khandare, Rahul V; Jadhav, Jyoti P; Govindwar, Sanjay P

    2016-06-01

    Ipomoea aquatica, a macrophyte was found to degrade a highly sulfonated and diazo textile dye Brown 5R up to 94% within 72 h at a concentration of 200 mg L(-1). Induction in the activities of enzymes such as azoreductase, lignin peroxidase, laccase, DCIP reductase, tyrosinase, veratryl alcohol oxidase, catalase and superoxide dismutase was observed in leaf and root tissue in response to Brown 5R exposure. There was significant reduction in contents of chlorophyll a (25%), chlorophyll b (17%) and carotenoids (30%) in the leaves of plants. HPLC, FTIR, UV-vis spectrophotometric and HPTLC analyses confirmed the biotransformation and removal of parent dye from solution. Enzymes activities and GC-MS analysis of degradation products lead to the proposal of a possible pathway of phytotransformation of dye. The proposed pathway of dye metabolism revealed the formation of Napthalene-1,2-diamine and methylbenzene. Toxicity study on HepG2 cell lines showed a 3 fold decrease in toxicity of Brown 5R after phytoremediation by I. aquatica. Hydrophytic nature of I. aquatica leads to its exploration in a combinatorial phytoreactor with Ipomoea hederifolia soil bed system. Rhizofiltration with I. aquatica and soil bed treatment by I. hederifolia treated 510 L of effluent effectively within 72 h. I. aquatica along with I. hederifolia could decolorize textile industry effluent within 72 h of treatment as evident from the significant reductions in the values of COD, BOD, solids and ADMI. Further on field trials of treatment of textile wastewater was successfully carried out in a constructed lagoon.

  14. Toxicity of Zinc on Growth of an Aquatic Macrophyte, Ipomoea Aquatica Forsk

    OpenAIRE

    Laitonjam Bedabati Chanu; Abhik Gupta

    2016-01-01

    The effects of different concentrations of Zn on growth of an aquatic macrophyte, Ipomoea aquatica Forsk. were studied. Fresh weight, dry weight, shoot length, root length, number of nodes, development of leaves, and chlorophyll and carotene contents were the different growth parameters considered. Toxicity symptoms like browning and decaying of roots could be observed in plants treated at 22.7 mg L-1 Zn as early as 3rd day of experiment while yellowing of older leaves appeared during the lat...

  15. CNS Depressant and Antiepileptic Activities of the Methanol Extract of the Leaves of Ipomoea Aquatica Forsk

    OpenAIRE

    Dhanasekaran Sivaraman; Palayan Muralidaran

    2010-01-01

    The central nervous system (CNS) depressant and antiepileptic activities of the methanol extract of the leaves of Ipomoea aquatica Forsk (IAF) were investigated on various animal models including pentobarbitone sleeping time and hole-board exploratory behavior for sedation tests and strychnine, picrotoxin and pentylenetetrazole-induced convulsions in mice. IAF (200 and 400 mg/kg, p.o.), like chlorpromazine HCl (1 mg/kg, i.m.), produced a dose-dependent prolongation of pentobarbitone sleeping ...

  16. Ipomoea aquatica Extract Shows Protective Action Against Thioacetamide-Induced Hepatotoxicity

    Directory of Open Access Journals (Sweden)

    A. Hamid A. Hadi

    2012-05-01

    Full Text Available In the Indian system of traditional medicine (Ayurveda it is recommended to consume Ipomoea aquatica to mitigate disorders like jaundice. In this study, the protective effects of ethanol extract of I. aquatica against liver damage were evaluated in thioacetamide (TAA-induced chronic hepatotoxicity in rats. There was no sign of toxicity in the acute toxicity study, in which Sprague-Dawley (SD rats were orally fed with I. aquatica (250 and 500 mg/kg for two months along with administration of TAA (i.p injection 200 mg/kg three times a week for two months. The results showed that the treatment of I. aquatica significantly lowered the TAA-induced serum levels of hepatic enzyme markers (ALP, ALT, AST, protein, albumin, bilirubin and prothrombin time. The hepatic content of activities and expressions SOD and CAT that were reduced by TAA were brought back to control levels by the plant extract supplement. Meanwhile, the rise in MDA level in the TAA receiving groups also were significantly reduced by I. aquatica treatment. Histopathology of hepatic tissues by H&E and Masson trichrome stains displayed that I. aquatica has reduced the incidence of liver lesions, including hepatic cells cloudy swelling, infiltration, hepatic necrosis, and fibrous connective tissue proliferation induced by TAA in rats. Therefore, the results of this study show that the protective effect of I. aquatica in TAA-induced liver damage might be contributed to its modulation on detoxification enzymes and its antioxidant and free radical scavenger effects. Moreover, it confirms a scientific basis for the traditional use of I. aquatica for the treatment of liver disorders.

  17. Ipomoea aquatica extract shows protective action against thioacetamide-induced hepatotoxicity.

    Science.gov (United States)

    Alkiyumi, Salim Said; Abdullah, Mahmood Ameen; Alrashdi, Ahmed Salim; Salama, Suzy Munir; Abdelwahab, Siddig Ibrahim; Hadi, A Hamid A

    2012-05-22

    In the Indian system of traditional medicine (Ayurveda) it is recommended to consume Ipomoea aquatica to mitigate disorders like jaundice. In this study, the protective effects of ethanol extract of I. aquatica against liver damage were evaluated in thioacetamide (TAA)-induced chronic hepatotoxicity in rats. There was no sign of toxicity in the acute toxicity study, in which Sprague-Dawley (SD) rats were orally fed with I. aquatica (250 and 500 mg/kg) for two months along with administration of TAA (i.p injection 200 mg/kg three times a week for two months). The results showed that the treatment of I. aquatica significantly lowered the TAA-induced serum levels of hepatic enzyme markers (ALP, ALT, AST, protein, albumin, bilirubin and prothrombin time). The hepatic content of activities and expressions SOD and CAT that were reduced by TAA were brought back to control levels by the plant extract supplement. Meanwhile, the rise in MDA level in the TAA receiving groups also were significantly reduced by I. aquatica treatment. Histopathology of hepatic tissues by H&E and Masson trichrome stains displayed that I. aquatica has reduced the incidence of liver lesions, including hepatic cells cloudy swelling, infiltration, hepatic necrosis, and fibrous connective tissue proliferation induced by TAA in rats. Therefore, the results of this study show that the protective effect of I. aquatica in TAA-induced liver damage might be contributed to its modulation on detoxification enzymes and its antioxidant and free radical scavenger effects. Moreover, it confirms a scientific basis for the traditional use of I. aquatica for the treatment of liver disorders.

  18. Antioxidant and α-Glucosidase Inhibitory Activities of Isolated Compounds from Ipomoea aquatica

    Directory of Open Access Journals (Sweden)

    Umar Lawal

    2016-05-01

    Full Text Available Ipomoea aquatica Forsk is a green leafy vegetable that is a rich source of amino acids and vitamins. Antioxidant and α-glucosidase inhibitory activities of the hexane (IAHE and methanol (IAME fractions of the vegetable portion of I. aquatica were investigated. The IAME fraction exhibited a strong scavenging effect of the 2,2-diphenyl-2-picryl hydrazyl (DPPH free radicals, and this fraction contained the highest phenolic contents. Phytochemical investigation of the IAME fraction yielded three known compounds, namely 3,5-di-O-caffeoylquinic acid (1, 4,5-di-O-caffeoylquinic acid (2 and quercetin 3-O-β-D-glucoside (3. The structures were unambiguously elucidated based on 1D and 2D-NMR analyses (1H, 13C, COSY, HSQC, HMBC and mass spectrometry data. Compound 3 was isolated for the first time from this plant.

  19. Bioaccumulations of heavy metals in Ipomoea aquatica grown in bottom ash recycling wastewater.

    Science.gov (United States)

    Milla, Odette Varela; Rivera, Eva B; Huang, Wu-Jang

    2014-05-01

    A plant bioassay using hydroponically grown Ipomoea aquatica (water spinach) was applied to assess the phytotoxicity of untreated and treated wastewaters from a municipal solid waste incineration bottom ash recycling facility. The 50%-diluted, untreated wastewater exhibited acute toxicity (plants died within 24 hours). Highly diluted doses (3 and 6%) of both wastewater types displayed no significant differences when compared with the control. Treating the wastewater through sequential physical filtration and chemical precipitation processes decreased not only the dissolved solids content but also the pH and salt content. In addition, significant accumulations of Sr, Cr, and Sn were observed in the hydroponically grown I. aquatica plant tissues; in particular, the bioaccumulation of Sr in the leaves and roots was unexpectedly high.

  20. Exploration of possible mechanisms for anti-inflammatory activity of Ipomoea aquatica Forsk. (Convolvulaceae

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    Mital N. Manvar

    2015-11-01

    Full Text Available Currently used steroidal and non steroidal anti-inflammatory drugs have severe side effects. These side effects are very difficult to manage than the disease itself. Hence, there is to search new safe resources to cure such diseases that the use of plant based drugs. This study deals with anti-inflammatory evaluation of the hydroalcoholic extract of Ipomoea aquatica leaves as well as their possible mechanism of action. A carrageenan‐induced rat paw oedema model was used for anti-inflammatory study. The mechanism/s by which Ipomoea aquatica is mediated the ant-inflammatory activity was determined by its effects in antihistamine activity, prostaglandin synthesis inhibition activity, membrane stabilizing activity and protein denaturation inhibition activity. Dose dependent anti-inflammatory activity was found with HAEIA in rat paw oedema model using carrageenan. HAEIA effective to suppressed the wheal area formed by histamine. HAEIA revealed dose dependent prostaglandin synthesis inhibition activity. HAEIA was effectively inhibited the heat induced hemolysis of HRBCs as well as heat induced albumin denaturation. Therefore, it was concluded that the HAEIA has anti-inflammatory activity possibly mediated through inhibition of release of mediator histamine and prostaglandin and has also HRBCs membrane stabilization and protein denaturation inhibition properties.

  1. Enhanced nitrogen and phosphorus removal from eutrophic lake water by Ipomoea aquatica with low-energy ion implantation.

    Science.gov (United States)

    Li, Miao; Wu, Yue-Jin; Yu, Zeng-Liang; Sheng, Guo-Ping; Yu, Han-Qing

    2009-03-01

    Ipomoea aquatica with low-energy N+ ion implantation was used for the removal of both nitrogen and phosphorus from the eutrophic Chaohu Lake, China. The biomass growth, nitrate reductase and peroxidase activities of the implanted I. aquatica were found to be higher than those of I. aquatica without ion implantation. Higher NO3-N and PO4-P removal efficiencies were obtained for the I. aquatica irradiation at 25 keV, 3.9 x 10(16) N+ ions/cm(2) and 20 keV 5.2 x 10(16) N+ ions/cm(2), respectively (p phosphorus contents in the plant biomass with ion implantation were also greater than those of the controls. I. aquatica with ion implantation was directly responsible for 51-68% N removal and 54-71% P removal in the three experiments. The results further confirm that the ion implantation could enhance the growth potential of I. aquatica in real eutrophic water and increase its nutrient removal efficiency. Thus, the low-energy ion implantation for aquatic plants could be considered as an approach for in situ phytoremediation and bioremediation of eutrophic waters.

  2. Effect of Arbuscular Mycorrhiza Fungi Inoculation on Growth and Up take of Mineral Nutrition in Ipomoea Aquatica.

    Directory of Open Access Journals (Sweden)

    Milton Halder

    2015-04-01

    Full Text Available A green house experiment was conducted to investigate the effect of arbuscular mycorrhiza inoculation on plant growth and uptake of mineral nutrition in Ipomoea aquatica considering the objective of using environmental friendly biofertilizer instead of chemical fertilizer. A common leafy vegetable plant Ipomoea aquatica was grown with mycorrhiza and without mycorrhiza for 42 days. After harvest the plants were analyzed for mineral nutrition concentration. Plant fresh weight, dry weight, macronutrient (P, K, Mg, Na, micronutrient (Fe, Mn, Zn concentration was higher in arbuscular mycorrhiza inoculated plant than non-mycorrhiza inoculated plant. For sustainable agriculture, introducing biofertilizer by using arbuscular mycorrhiza inoculation would be one of the most efficient techniques for replacing chemical fertilizer to meet the nutrient deficiency in nutrient deficient soils of Bangladesh.

  3. FITOREMEDIASI KADMIUM (CD PADA LEACHATE MENGGUNAKAN KANGKUNG AIR (Ipomoea aquatica Forsk. (STUDI KASUS TPA JATIBARANG

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    K N Zamhar

    2016-03-01

    Full Text Available Leachate TPA Jatibarang yang tercemar kadmium (Cd kemungkinan dapat dibersihkan secara fitoremediasi menggunakan kangkung air (Ipomoea aquatica Forsk.. Penelitian ini bertujuan untuk mengetahui kemampuan kangkung air sebagai fitoremediator dalam menyerap Cd dalam leachate TPA Jatibarang. Sebelum penelitian, dilakukan uji pendahuluan kandungan Cd dalam leachate maupun dalam kangkung air. Kangkung diaklimatisasi dalam air bersih selama 3 hari, selanjutnya ditimbang seberat 300 gram kemudian diletakkan ke dalam ember berisi 10 L leachate. Sampel leachate dan organ tanaman diambil setiap 2 hari sekali (2 hari, 4 hari, 6 hari, dan 8 hari. Faktor intensitas cahaya diambil setiap hari pada pukul 08.00-09.00 WIB menggunakan lux meter, sedangkan nilai pH dan suhu leachate diukur menggunakan kertas indikator pH dan termometer sebelum dan sesudah perlakuan. Perameter yang diamati adalah akumulasi Cd dalam akar, batang, dan daun kangkung air pada masing-masing lama waktu pananaman. Akumulasi Cd diukur dengan AAS (Atomic Absorption Spectrophotometry. Data penyerapan Cd dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa lama waktu kangkung air ditanam dalam leachate berpengaruh terhadap akumulasi Cd pada tanaman. Penyerapan Cd oleh kangkung air mencapai jenuh pada hari ke-8 dengan total penyerapan 0,052 ppm. Akumulasi Cd paling besar pada akar kangkung yaitu 0,023 ppm.Cadmium (Cd that contained in TPA Jatibarang’s leachate can be cleaned by phytoremediation using water spinach (Ipomoea aquatica Forsk.. This research aimed to determine the ability of the water spinach as a fitoremediator in adsorb cadmium (Cd that contained in TPA Jatibarang’s leachate. The research was conducted by using a randomized completely design (RCD factorial, with longer treatment planted time in leachate water spinach was 0 day, 2 days, 4 days, 6 days, and 8 days respectively. The observed parameters of Cd accumulation in roots, stems, and leaves of water spinach in

  4. CNS Depressant and Antiepileptic Activities of the Methanol Extract of the Leaves of Ipomoea Aquatica Forsk

    Directory of Open Access Journals (Sweden)

    Dhanasekaran Sivaraman

    2010-01-01

    Full Text Available The central nervous system (CNS depressant and antiepileptic activities of the methanol extract of the leaves of Ipomoea aquatica Forsk (IAF were investigated on various animal models including pentobarbitone sleeping time and hole-board exploratory behavior for sedation tests and strychnine, picrotoxin and pentylenetetrazole-induced convulsions in mice. IAF (200 and 400 mg/kg, p.o., like chlorpromazine HCl (1 mg/kg, i.m., produced a dose-dependent prolongation of pentobarbitone sleeping time and suppression of exploratory behavior. IAF (200 and 400 mg/kg produced dose-dependent and significant increases in onset to clonic and tonic convulsions and at 400 mg/kg, showed complete protection against seizures induced by strychnine and picrotoxin but not with pentylenetetrazole. Acute oral toxicity test, up to 14 days, did not produce any visible signs of toxicity. These results suggest that potentially antiepileptic compounds are present in leaf extract of IAF that deserve the study of their identity and mechanism of action.

  5. Element contents and food safety of water spinach (Ipomoea aquatica Forssk.) cultivated with wastewater in Hanoi, Vietnam

    DEFF Research Database (Denmark)

    Marcussen, Helle; Jørgensen, Karin; Holm, Peter E.;

    2008-01-01

    Extensive aquatic or semi-aquatic production of water spinach (Ipomoea aquatica Forssk.) for human consumption takes place in Southeast Asia. The aim of this study was to assess the concentrations of 38 elements in soil and water spinach cultivated under different degrees of wastewater exposure...... in Hanoi, Vietnam. The results showed no effect of wastewater use on the overall element concentrations in soil and water spinach. Mean soil concentrations for selected potentially toxic elements at the studied field sites had the following ranges 9.11-18.7 As, 0.333-0.667 Cd, 10.8-14.5 Co, 68-122 Cr, 34...

  6. Damage suffered by swamp morning glory (Ipomoea aquatica Forsk) exposed to vanadium (V).

    Science.gov (United States)

    Chen, Ting; Li, Ting-Qiang; Yang, Jin-Yan

    2016-03-01

    To elucidate the physiological and morphological responses generated by vanadium (V) in plants, hydroponic culture experiments were performed with swamp morning glory (Ipomoea aquatica Forsk) exposed to 0 mg L(-1) to 2.50 mg L(-1) pentavalent V [V(V)] in Hoagland nutrient solutions. The concentration of chlorophyll a, chlorophyll b, and carotene peaked at a V(V) concentration of 0.05 mg L(-1) and gradually decreased at higher V(V) concentrations. Similarly, the plant biomass was stimulated at low levels of V(V) and was inhibited when V(V) concentrations exceeded 0.1 mg L(-1). Pentavalent V had negative effects on the uptake of phosphorus (P) by roots, shoots, and leaves. The biological absorption coefficients of V of the roots were higher than those of the aerial parts. Under low concentrations of V(V) exposure, the predominant species of V in the aerial parts was tetravalent V [V(IV)], whereas V(V) became more prevalent when concentrations of V(V) in the solution was higher than 0.50 mg L(-1). In the roots, however, the concentrations of V(V) were always higher than those of the V(IV), except in the control group. Organelles in the V(V)-treated leaves were distorted, and the periplasmic space became wider. These results indicate V(V) has concentration-dependent effects on the physiological properties of swamp morning glory, whereas the plant has the ability to develop self-protective function to adapt to the toxicity of V(V).

  7. Faecal and protozoan parasite contamination of water spinach (Ipomoea aquatica) cultivated in urban wastewater in Phnom Penh, Cambodia

    DEFF Research Database (Denmark)

    Anh, Vuong Tuan; Tram, Nguyen Thuy; Klank, Lise Tønner

    2007-01-01

    Objective To identify the level of contamination with thermotolerant coliforms (ThC), intestinal helminth eggs and protozoan parasites in water spinach (Ipomoea aquatica) cultivated in a wastewater-fed lake in Phnom Penh, Cambodia. Methods The investigation was carried out from July 2004 to May...... into a stream. Water spinach samples were harvested at each of the three locations with and without wastewater contact according to the normal practices of farmers, and analysed for ThC, protozoan parasites (Giardia, Cryptosporidium and Cyclospora), and helminth eggs (Ascaris lumbricoides, Trichuris trichura...... and hookworm) using standard methods. Wastewater samples were also collected at each of the three locations and analysed for ThC and helminth eggs. Results High concentrations of ThC (approximately 10(5)-10(7)/g) were found in water spinach samples. ThC mean counts did not differ significantly, neither between...

  8. Protection against carbofuran-induced toxicity in rat tissues and plasma by Ipomoea aquatica Forsk crude extract

    Directory of Open Access Journals (Sweden)

    Sanjukta Datta

    2013-08-01

    Full Text Available Objective: Carbofuran (2,3-dihydro-2,2-dimethyl-7-benzofuranyl methylcarbamate, a commonly used carbamate, induces oxidative stress through free radical generation. Role of green leafy vegetables against such toxic compounds have been well established. Hence, this study aimed to evaluate the deleterious effects of carbofuran on brain and plasma in albino male rats of Charles Foster strain and whether Ipomoea aquatica crude extract (IAE can protect body cells and tissues against oxidative insult. Methods: The rats were divided into 4 groups: one was treated with an oral dose of 0.1 mg/kg b.wt of carbofuran alone; 20 mg of polyphenolic compound expressed as gallic acid equivalents per kg b.wt was fed to another group; a third group was gavaged both the doses; the final group served as control and provided normal diet. Results: Evaluations based on altered activities of antioxidant enzymes superoxide dismutase, catalase, glutathione peroxidase and non-enzymatic glutathione in carbofuran treated rats showed the protective side of IAE. Also, increase in the total cholesterol levels in brain and plasma and DNA fragmentation in bone marrow cells were attenuated positively in the presence of IAE. Conclusion: The present study gives an insight into the protective role of plant polyphenols in minimizing the ill-effects of carbofuran. [J Exp Integr Med 2013; 3(4.000: 323-329

  9. Protective effect of secondary plant metabolites from Ipomoea aquatica Forsk. against carbofuran induced damages.

    Science.gov (United States)

    Datta, Sanjukta; Sinha, Mahuya; Das, Dipesh; Ghosh, Santinath; Dhar, Pubali

    2013-12-01

    Plausible interactions between food contaminants and natural constituents in vivo and protective effect of polyphenols present in I. aquatica against carbofuran toxicity in Charles Foster rats were evaluated. Determinations based on antioxidant enzyme activities showed significant alterations in glutathione, glutathione peroxidase, superoxide dismutase and catalase in tissues (liver and brain) and plasma of pesticide treated group while polyphenolic extracts from I. aquatica (IAE) attenuated their activities when given alongwith carbofuran. IAE decreased enhanced lipid peroxidation levels in plasma and erythrocyte membrane and cholesterol levels in brain and plasma. IAE also minimized histopathological degenerative changes produced by carbofuran. While single cell gel electrophoresis showed that secondary metabolites in leafy vegetables produced a combinatorial effect with pesticide at cellular level, DNA fragmentation level in bone marrow cells showed a decline in the IAE treated rats. Food safety adversely affected by various chemical contaminants can be retained by plant polyphenols and secondary plant constituents that can be found together in bolus. Therefore, the present study gives an insight into the protective role of naturally found polyphenols against pesticide toxicity.

  10. Effects of inorganic nitrogen forms on growth, morphology, nitrogen uptake capacity and nutrient allocation of four tropical aquatic macrophytes (Salvinia cucullata, Ipomoea aquatica, Cyperus involucratus and Vetiveria zizanioides)

    DEFF Research Database (Denmark)

    Jampeetong, Arunothai; Brix, Hans; Kantawanichkul, Suwasa

    2012-01-01

    with species and tissue, but differences between treatments were generally small. We conclude, that the free-floating S. cucullata and I. aquatica are good candidate species for use in constructed wetland systems to remove N from polluted water. The rooted emergent plants can be used in subsurface flow......This study assesses the growth and morphological responses, nitrogen uptake and nutrient allocation in four aquatic macrophytes when supplied with different inorganic nitrogen treatments (1) NH4+, (2) NO3−, or (3) both NH4+ and NO3−. Two free-floating species (Salvinia cucullata Roxb. ex Bory...... and Ipomoea aquatica Forssk.) and two emergent species (Cyperus involucratus Rottb. and Vetiveria zizanioides (L.) Nash ex Small) were grown with these N treatments at equimolar concentrations (500 M). Overall, the plants responded well to NH4+. Growth as RGR was highest in S. cucullata (0.12±0.003 d−1...

  11. Inexpensive Ipomoea aquatica Biomass-Modified Carbon Black as an Active Pt-Free Electrocatalyst for Oxygen Reduction Reaction in an Alkaline Medium

    Directory of Open Access Journals (Sweden)

    Yaqiong Zhang

    2015-09-01

    Full Text Available The development of inexpensive and active Pt-free catalysts as an alternative to Pt-based catalysts for oxygen reduction reaction (ORR is an essential prerequisite for fuel cell commercialization. In this paper, we report a strategy for the design of a new Fe–N/C electrocatalyst derived from the co-pyrolysis of Ipomoea aquatica biomass, carbon black (Vulcan XC-72R and FeCl3·6H2O at 900 °C under nitrogen atmosphere. Electrochemical results show that the Fe–N/C catalyst exhibits higher electrocatalytic activity for ORR, longer durability and higher tolerance to methanol compared to a commercial Pt/C catalyst (40 wt % in an alkaline medium. In particular, Fe–N/C presents an onset potential of 0.05 V (vs. Hg/HgO for ORR in an alkaline medium, with an electron transfer number (n of ~3.90, which is close to that of Pt/C. Our results confirm that the catalyst derived from I. aquatica and carbon black is a promising non-noble metal catalyst as an alternative to commercial Pt/C catalysts.

  12. Field crops (Ipomoea aquatica Forsk. and Brassica chinensis L.) for phytoremediation of cadmium and nitrate co-contaminated soils via rotation with Sedum alfredii Hance.

    Science.gov (United States)

    Tang, Lin; Luo, Weijun; Chen, Weikang; He, Zhenli; Gurajala, Hanumanth Kumar; Hamid, Yasir; Deng, Meihua; Yang, Xiaoe

    2017-07-01

    Phytoremediation coupled with crop rotation (PCC) is a feasible strategy for remediation of contaminated soil without interrupting crop production. The objective of this study was to develop a PCC technology system for greenhouse fields co-contaminated with Cd and nitrate using hyperaccumulator Sedum alfredii. In this system, endophytic bacterium M002 inoculation, CO2 fertilization, and fermentation residue were continuously applied to improve the growth of S. alfredii, and low-accumulator Ipomoea aquatica and low-accumulator Brassica chinensis were rotated under reasonable water management. These comprehensive management practices were shown to increase S. alfredii biomass and Cd uptake and reduce Cd and nitrate concentration in I. aquatica and B. chinensis. This crop rotating system could remove 56.5% total Cd, 62.3% DTPA extractable Cd, and 65.4% nitrate, respectively, from the co-contaminated soil in 2 years of phytoremediation, and is an effective way of remediating moderately co-contaminated soil by Cd and nitrate.

  13. Toxicity of OTC to Ipomoea aquatica Forsk. and to microorganisms in a long-term sewage-irrigated farmland soil.

    Science.gov (United States)

    Ma, Tingting; Chen, Li'ke; Wu, Longhua; Christie, Peter; Luo, Yongming

    2016-08-01

    Water spinach (Ipomoea aquatic Forsk.) was selected to investigate the effects of oxytetracycline (OTC) on the toxicity of soil contaminated by long-term sewage irrigation. After acute toxicity test in petri dish at nine different OTC-spiked levels for 48 h, the germination rate was found to be generally inhibited in all treatments treated with OTC but the root elongation and activities of several antioxidant enzymes, superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were either forward or backward stimulated to varying extent. During a 60-day sub-chronic toxicity test by means of a pot experiment, activities of SOD, POD and CAT in both the leaf and root tissue at 25 mg OTC per kg soil (dry weight) and in root tissue at 1 mg OTC per kg soil (dry weight) were significantly different than those in other treatments, which also indicated the higher sensitivity of the root. The foliar photosynthetic rate, stomatal conductance and transpiration rate were all gradually inhibited in spite of elevated water use efficiency under the pressure of the different OTC concentrations, which were highly significant different at 10 mg OTC per kg soil (dry weight). Indices of soil microbial diversity at 4 mg OTC kg(-1) soil were significantly different from those of the control, indicating the potential adverse effects of OTC to soil microorganisms. The results suggest that the introduction of OTC could damage both plants and soil microorganisms, and during sub-chronic incubation, the sensitivity of different indices generally followed the order of root tissue antioxidant enzyme activities, soil microbial diversity indices, leaf photosynthesis-related index and leaf tissue enzyme antioxidant activities. In addition, the application of livestock and poultry manure containing pollutants like OTC in farmland soil, especially if the soil has been contaminated before, should be taken more seriously in the context of the current pursuit of increased agricultural

  14. Pengaruh Penyiraman dan Dosis Pemupukan terhadap Pertumbuhan Kangkung (Ipomoea reptans) pada Komposisi Media Tanam Tanah+Pasir

    OpenAIRE

    AHMAD RIFQI FAUZI

    2015-01-01

    Effect of Water Requirment and Fertilization on Growth of Kangkong (Ipomoea reptans) in media composition soil+sand. Kangkong plant is a tropical vegetable widely cultivated in Indonesia. Village has a wide adaptability to climate and soil in the tropics. This experiment aimed to see the effect of watering and fertilization on the growth of kangkong plants. Experiments was carried out in the garden experiment Cikabayan, IPB on March-April 2011. This experiment was arranged with randomized com...

  15. Nitrogen and phosphorus removal performance and kinetics absorption by Ipomoea aquatica%蕹菜对富营养化水体的氮磷去除及吸收动力学研究

    Institute of Scientific and Technical Information of China (English)

    张亚娟; 刘存歧; 李洪波; 吴亦红; 王亚斌

    2011-01-01

    以蕹菜(Ipomoea aquatica)为材料,采用改进的常规耗竭法,对氨氮、硝酸盐氮和无机磷的吸收动力学特性进行了研究.通过室内静态实验测定了蕹菜对富营养化水体的氮磷去除效率.用Michaelis-Menten方程来描述蕹菜对氨氮、硝酸盐氮和无机磷的吸收速率与溶液浓度的相互关系.结果表明:蕹菜对氨氮、硝酸盐氮和无机磷的最大吸收常数Vmax为23.59、2.88和1.52μmol/(g FW·h),亲和力常数Km为1.90、10.94和0.97μmol/L,可见蕹菜对氨氮的吸收速率和亲和力均高于硝酸盐氮,对无机磷的吸收速率较小;蕹菜对富营养化水体总氮、氨氮、总磷和有机磷的去除率分别为38.13%、44.85%、63.77%和53.95%;蕹菜对受试水体表现了良好的适应性和净化能力.%The absorption kinetics of ammonium, nitrate and phosphorus by Ipomoea aquatica were studied,after that ammonium, nitrate and inorganic phosphorus removal from eutrophic water was examined under indoor static simulation experimental condition. The relationship between the uptake rate and the concentration was depicted using the Michaelis-Menten equation. The purposes of this study were to provide a scientific foundation for the removal mechanism of ammonium ,nitrate and inorganic phosphorus from water. The results showed that the maximum velocities (Vmax) of absorption on ammonium, nitrate and inorganic phosphorus were 23.59, 2.88 and 1.52μmol/( g FW·h), the Michaelis constant(Km ) were 1.90,10. 94 and 0. 97μmol/L. The results of static state removals of TN, NH4+-N, TP, DOP by I aquatica were 38.13% ,44. 85% ,63.77% and 53.95% , respectively. It is proved that I. aquatica could be used to purify the eutrophicated water body.

  16. Identification of some Bioactive Metabolites in a Fractionated Methanol Extract from Ipomoea aquatica (Aerial Parts) through TLC, HPLC, UPLC-ESI-QTOF-MS and LC-SPE-NMR Fingerprints Analyses.

    Science.gov (United States)

    Hefny Gad, Mahmoud; Tuenter, Emmy; El-Sawi, Nagwa; Younes, Sabry; El-Ghadban, El-Mewafy; Demeyer, Kristiaan; Pieters, Luc; Vander Heyden, Yvan; Mangelings, Debby

    2017-08-04

    The plant species Ipomoea aquatica contains various bioactive constituents, e.g. phenols and flavonoids, which have several medical uses. All previous studies were executed in Asia; however, no reports are available from Africa, and the secondary metabolites of this plant species from Africa are still unknown. The present study aims finding suitable conditions to identify the bioactive compounds from different fractions. Chromatographic fingerprint profiles of different fractions were developed using high-performance liquid chromatography (HPLC) and then these conditions were transferred to thin-layer chromatography (TLC). Subsequently, the chemical structure of some bioactive compounds was elucidated using ultra-performance liquid chromatography-quadrupole time of flight-tandem mass spectrometry (UPLC-QTOF-MS) and liquid chromatography-solid phase extraction-nuclear magnetic resonance (LC-SPE-NMR) spectroscopy. The HPLC fingerprints, developed on two coupled Chromolith RP-18e columns, using a gradient mobile phase (methanol/water/trifluoroacetic acid, 5:95:0.05, v/v/v), showed more peaks than the TLC profile. The TLC fingerprint allows the identification of the types of chemical constituents, e.g. flavonoids. Two flavonoids (nicotiflorin and ramnazin-3-O-rutinoside) and two phenolic compounds (dihydroxybenzoic acid pentoside and di-pentoside) were tentatively identified by QTOF-MS, while NMR confirmed the structure of rutin and nicotiflorin. The HPLC and TLC results showed that HPLC fingerprints give more and better separated peaks, but TLC helped in determining the class of the active compounds in some fractions. Bioactive constituents were identified as well using MS and NMR analyses. Two flavonoids and two phenolic compounds were tentatively identified in this species for the first time, to the best of our knowledge. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd.

  17. 沼泽红假单胞菌的分离鉴定及对蕹菜生长的影响%Research on Isolation, Identification of Rhodopseudomonas palustris and Its Effects on the Growth of Ipomoea aquatica

    Institute of Scientific and Technical Information of China (English)

    黄小春; 王小东; 赵攀

    2014-01-01

    One strain of photosynthetic bacteria was enriched and isolated from soil amendment imported from Japan, named as GHR -507. The morphology and physiological -biochemical characteristics of this strain showed it belonged to Rhodopseudomonas palustris. With Ipomoea aquatica as the test material, and the leaves were sprayed by 500 times dilution of GHR-507 at young seedling stage, growth period, foliar application to spray water amount as controls. The plot yields were investigated in ripeness stage. The results showed that the production of first and second batch compared with antitheses increase 31.03%, 20.74%, respectively.%从引进的日本土壤改良剂中富集分离得到1株光合细菌,通过形态学观察及生理生化特性鉴定,鉴定为该菌株属于红假单胞菌属。以泰国柳叶蕹菜为试验材料,用GRH-507菌液稀释500倍在蕹菜幼苗期、生长期进行叶面喷施,以等量的清水作对照,在成熟期调查蕹菜的产量。结果表明,喷施500倍GRH-507菌液稀释液能提高蕹菜的产量,两次收割产量分别比对照增产31.03%、20.74%。

  18. Uptake of iodine-131 in tropical crops. [Ipomoea batatas; Ipomoea reptans; Lycopersicon

    Energy Technology Data Exchange (ETDEWEB)

    Asprer, G.A.; Lansangan, L.M.

    1986-01-01

    Vegetable crops which include sweet potato tops (Ipomoea batatas), kangkong (Ipomoea repitans) and tomato plants were grown in dark-painted jars containing Hoagland-Arnon modified nutrient solution, utilizing the technique of hydroponics. The experiments for sweet potato tops and kangkong plants were duplicated for replicate studies and steady-state conditions were simulated throughout. Tomato plants were grown in the same manner but growth was observed to be hampered when starting from mature plants. Radioiodine was added to the nutrient medium containing 0.5% non-radioactive NaI solution. The solution in the jar was adjusted daily so as to maintain a constant concentration which would simulate routine releases that are essentially continuous. After incorporating the radioiodine to the solution, 10 ml aliquot was taken and counted for radioactivity by means of a 5'' x 5'' NaI(T1) detector connected to the multichannel gamma analyzer. Both plants and solution were counted for radioactivity at different time intervals using the same geometry. Results indicate that the activity in the plants were relatively higher than that of the solution. The activity tends to level off or decrease after a few days. The concentration factor which is the ratio of the activity in the plant (uCi/gm) over the activity in the medium (uCi/ml) varied for each time interval. 12 references, 2 figures, 3 tables.

  19. 浮床水蕹菜对养殖水体中营养物的去除效果研究%In situ nutrient removal from aquaculture wastewater by the aquatic vegetable Ipomoea aquatica on floating beds

    Institute of Scientific and Technical Information of China (English)

    李文祥; 李为; 林明利; 王英雄; 刘家寿; 李钟杰

    2011-01-01

    To investigate the nutrient removal in an aquaponic system,pilot scale tests were carried out in six earthen ponds of area 2000 m2 from June to November in 2007.The aquatic vegetable Ipomoea aquatica(200 m2 area) was planted on floating beds in three ponds.The other three ponds without the vegetable were considered controls.The same fish weight was stocked in the planted and control ponds,but with different fish species.At the end of experiment,the fish production in planted ponds(1467 kg) was significantly higher than in control ponds(1203 kg)(p〈0.05).Percentage removal of total nitrogen(TN) and total phosphorus(TP) by harvested fish in the control ponds was 25.17% and 15.27%,respectively.Percentage removal of TN and TP by harvested fish in the planted ponds was 30.02% and 21.68%,respectively.The fresh weight of harvested vegetable was(4000±350) kg;and the percentage removal of TN and TP was 8.41% and 4.85%,respectively.In the middle and late period of the experiment,the contents of chemical oxygen demand(COD) and chlorophyll a(Chl a) in the planted ponds were significantly lower than in the control ponds,and the transparency was significantly higher than in the control ponds.The concentration of dissolved oxygen(DO) in planted ponds was higher than 5 mg · L-1 during the experiment,whereas the concentration of DO in the control ponds decreased sharply in the last month.The results suggested that the water quality could be improved effectively and a higher fish density could be stocked in the aquaponic system.%为研究浮床水蕹菜对池塘养殖水质的改善效果,于2007年6月至11月,在6个2000m2的标准养殖池塘进行了中试研究.3个池塘放养常规鱼类和部分名优鱼类,并利用浮床技术在水面上种植200m2的水蕹菜(Ipomoea aquatica),另外3个池塘放养相同重量的常规鱼类作为对照塘.实验结果显示,种植塘的渔产量为1467kg,显著高于对照塘1203kg

  20. Pengaruh Penyiraman dan Dosis Pemupukan terhadap Pertumbuhan Kangkung (Ipomoea reptans pada Komposisi Media Tanam Tanah+Pasir

    Directory of Open Access Journals (Sweden)

    AHMAD RIFQI FAUZI

    2015-09-01

    Full Text Available Effect of Water Requirment and Fertilization on Growth of Kangkong (Ipomoea reptans in media composition soil+sand. Kangkong plant is a tropical vegetable widely cultivated in Indonesia. Village has a wide adaptability to climate and soil in the tropics. This experiment aimed to see the effect of watering and fertilization on the growth of kangkong plants. Experiments was carried out in the garden experiment Cikabayan, IPB on March-April 2011. This experiment was arranged with randomized complete design which consisted of two factors are 3 levels of watering (watering every 1 day (A1, every 2 days (A2, and every 3 days (A3 and fertilization with 5 doses (100% dose recommendation (P1, 75% (P2 , 50% (P3, 25% (P4, and 0 (P5. Planting medium was a mixture of soil : sand (1:1. The results of this study indicate that the frequency of watering significant effect on plant height and weight of wet kangkong plants. Watering once every 2 days to produce the highest plant height (41.41 cm. While fertilization had no effect on all observed variables. Conclusion of the experiment is watering is one of important factor for plant production which significant effect on plant growth.

  1. SYSTEMATIC STUDIES IN SOME IPOMOEA LINN. SPECIES USING POLLEN AND FLOWER MORPHOLOGY

    Directory of Open Access Journals (Sweden)

    Olatunde R. OLADUNJOYE

    2012-01-01

    Full Text Available The current study was conducted in search of constant morpho-anatomical characters to aid the identification and classification of commonly encountered Ipomoea species in south west Nigeria. Flower and pollen morphology as important repository of constant characters formed the focus of the investigation. Whole flowers were dissected to expose the carpel and stamens for study. For pollen study, after soaking in de-ionized water, the anthers were collected and crushed, stained and placed on a glass slide by needle for observation under light microscope. The lengths of styles and filament all varied in the seven species, highest length in styles was recorded in Ipomoea hederifolia (37.0-38.5mm while the minimum was recorded in Ipomoea vagans (16.5-19.0mm. United bract into a boat-shaped, doubly acuminate involucre distinguished Ipomoea involucrata from the remaining six species with free bracts. Pollen grains were found to be radially symmetrical; circular in outline, sculpture were echinate, circular aperture, pores equidistantly distributed, oblate, speriodal and oblate-spheriodal. Largest pollen size was recorded in Ipomoea aquatica (60.2-62.5µm, suggesting a less derived position whereas the minimum size (30.7-31.4µm was observed in Ipomoea hederifolia. The maximum spine length was recorded in Ipomoea involucrate (8.3-9.6µm and minimum was recorded in Ipomoea hederifolia (3.3-4.0µm. The phylogenetic tree resulting from cluster analysis suggests that the alien species I. aserifolia and I. carnea are sisters to the other native Ipomoea species. As the number of species increases, synapomorphies decrease whereas increased synapomorphies are associated with decreasing number of species.While both I. aserifolia and I. carnea may have naturalized, they represent new records for the Flora of West Tropical Africa.

  2. 水雍菜浮床养殖模式下黄颡鱼肠道菌群结构分析%Intestinal microbial communities of yellow catfish (Pseudobagrus fulvidraco) in pond with Ipomoea aquatica forsk planted on artificial floating beds

    Institute of Scientific and Technical Information of China (English)

    石焱; 马徐发; 吴志新; 任雨薇; 陈孝煊

    2016-01-01

    The removal capacity of nitrogen and phosphorus by I pomoea aquatica Forsk planted on artificial floating beds were emphatically studied,and the microflora structure in the yellow catfish (P seudobagrus fulvidraco )intestine were analyzed by using high-through sequencing technique.The re-sults showed that ammonia nitrogen,total nitrogen and total phosphorus can be removed by the I .aquatica Forsk planted on artificial floating beds,with high removal efficiency.There were some differences in the intestinal bacteria diversity between the experimental (with artificial floating beds)and control (without artificial floating beds)groups.But the bacterial composition of different groups did not significantly vary from each other,and the predominant species were similar in all groups.At the begin-ning period,Proteobacteria was dominant (88.56%)in the yellow catfish intestine,and at the ending pe-riod,the dominant bacterial community in the control group were Firmicutes (48.4%),Fusobacteria (32.52%)and Proteobacteria (14.56%),whereas in the experimental group,the core microbiota com-posed of Proteobacteria (36.72%),Firmicutes (21.89%),Fusobacteria (17.48%)and Bacteroidetes (1 1.48%).This study provided a more comprehensive understanding that I .aquatica Forsk planted on artificial floating beds can change the flora structure of the yellow catfish intestine.%通过大规模养殖试验,研究人工浮床水培水雍菜对鱼塘养殖废水中氮磷的去除能力,并采用高通量测序方法研究养殖前后和不同处理组黄颡鱼肠道的微生物菌群结构,并分析了它们之间的相关性。结果表明:人工浮床水培水雍菜对鱼塘养殖废水中氨氮、总氮和总磷有良好的去除作用。养殖前后黄颡鱼肠道细菌多样性有明显差异;不同处理组之间的黄颡鱼肠道细菌种类多样性存在一定的差异,但优势菌群的组成并没有明显的结构差异,优势菌种的构成相似。在养殖前期

  3. Aquaponics Cultivation of Ipome Aquatica and the Peasant Financial Income of Cirata Cistern

    Directory of Open Access Journals (Sweden)

    Abel Gandhy

    2016-08-01

    Full Text Available The research paper analyzes the financial income of a poor community living around the Cirata cistern, in West Java, Indonesia, before and after the community employed the aquaponics strategy of planting ipomoea aquatica. Data are gathered from observation, interviews and statistical analysis of the incomes of community members. In addition, analysis of the data applies Net Present Value (NPV, Internal Rate of Return (IRR, and Payback Period (PP. The findings clearly demonstrate an increased annual income of more than 1.117.296 rupiah among those community members using the aquaponics strategy. Once the community has adjusted to professionally implementing this aquaponics strategy, it would be practically useful to invite investors to invest their capital to develop this area.   Keywords: aquaponics, financial income, West Java, internal rate of return, payback period

  4. 蕹菜有机水培试验%Organic Hydroponics of Ipomoea aquatica Forsk

    Institute of Scientific and Technical Information of China (English)

    罗林会; 王勤

    2010-01-01

    采用水培方法,研究发酵油菜籽油粕原液稀释成4种浓度的油粕营养液对蕹菜生长发育的影响.试验结果表明.10%油菜籽油粕原液除地下部鲜质量、干质量两项指标稍低外,蕹莱的地上部鲜质量和干质量、最大枝长、最大叶面积、总叶绿素含量均为最大,且可食部分产量最高.由此可见,10%油粕营养液浓度是一种比较适宜有机水培蕹菜的菜籽油粕原液稀释浓度.

  5. Heavy metals phyto-assessment in commonly grown vegetables: water spinach (I. aquatica) and okra (A. esculentus).

    Science.gov (United States)

    Ng, Chuck Chuan; Rahman, Md Motior; Boyce, Amru Nasrulhaq; Abas, Mhd Radzi

    2016-01-01

    The growth response, metal tolerance and phytoaccumulation properties of water spinach (Ipomoea aquatica) and okra (Abelmoschus esculentus) were assessed under different contaminated spiked metals: control, 50 mg Pb/kg soil, 50 mg Zn/kg soil and 50 mg Cu/kg soil. The availability of Pb, Zn and Cu metals in both soil and plants were detected using flame atomic absorption spectrometry. The concentration and accumulation of heavy metals from soil to roots and shoots (edible parts) were evaluated in terms of translocation factor, accumulation factor and tolerance index. Okra recorded the highest accumulation of Pb (80.20 mg/kg) in its root followed by Zn in roots (35.70 mg/kg) and shoots (34.80 mg/kg) of water spinach, respectively. Different accumulation trends were observed with, Pb > Zn > Cu in okra and Zn > Pb > Cu in water spinach. Significant differences (p < 0.01) of Pb, Zn and Cu accumulation were found in both water spinach and okra cultivated among tested treatments. However, only the accumulation of Pb metal in the shoots of water spinach and okra exceeded the maximum permissible levels of the national Malaysian Food Act 1983 and Food Regulations 1985 (2006) as well as the international Codex Alimentarius Commission limits. This study has shown that both water spinach and okra have good potential as Pb and Zn phytoremediators.

  6. Roseomonas aquatica sp. nov., isolated from drinking water.

    Science.gov (United States)

    Gallego, Virginia; Sánchez-Porro, Cristina; García, Maria Teresa; Ventosa, Antonio

    2006-10-01

    Strain TR53(T), a Gram-negative, non-motile, non-spore-forming and strictly aerobic coccobacillus, isolated from the drinking water distribution system of Seville, Spain, was subjected to polyphasic taxonomic analysis using a combination of phenotypic, genotypic and phylogenetic features. Phylogenetic analysis of 16S rRNA gene sequences showed that strain TR53(T) had highest similarity to members of the genus Roseomonas, with sequence similarity values between 95.7 % (to Roseomonas genomospecies 5 strain ATCC 49960) and 94.0 % (to Roseomonas gilardii subsp. rosea ATCC 49956(T)). On the basis of its phenotypic characteristics, 16S rRNA gene sequence data and DNA G+C content (68.6 mol%), strain TR53(T) represents a novel species of the genus Roseomonas, for which the name Roseomonas aquatica sp. nov. is proposed. The type strain of Roseomonas aquatica is TR53(T) (=CECT 7131(T)=JCM 13556(T)).

  7. Chrysolina herbacea modulates terpenoid biosynthesis of Mentha aquatica L.

    Directory of Open Access Journals (Sweden)

    Simon Atsbaha Zebelo

    Full Text Available Interactions between herbivorous insects and plants storing terpenoids are poorly understood. This study describes the ability of Chrysolina herbacea to use volatiles emitted by undamaged Mentha aquatica plants as attractants and the plant's response to herbivory, which involves the production of deterrent molecules. Emitted plant volatiles were analyzed by GC-MS. The insect's response to plant volatiles was tested by Y-tube olfactometer bioassays. Total RNA was extracted from control plants, mechanically damaged leaves, and leaves damaged by herbivores. The terpenoid quantitative gene expressions (qPCR were then assayed. Upon herbivory, M. aquatica synthesizes and emits (+-menthofuran, which acts as a deterrent to C. herbacea. Herbivory was found to up-regulate the expression of genes involved in terpenoid biosynthesis. The increased emission of (+-menthofuran was correlated with the upregulation of (+-menthofuran synthase.

  8. Phosphorus correlation study for vegetables grown in the ultisol: Nanggung, Bogor, Indonesia

    OpenAIRE

    Kartika, J.G.; Anas D Susila

    2008-01-01

    Phosphorus (P) correlation experiment has been done in Ultisol soil in Nanggung District, Bogor, Indonesia from 2006-2007 to find the best P extraction method which suitable for vegetables i.e Amaranth (Amaranthus sp), Kangkong (Ipomoea aquatica L), Eggplant (Solanum melongena L), Chili (Capsicum annuum L), Tomato (Lycopersicon esculentum Mill), Green Bean (Pahaseolus vulgaris L), and Yard Long Bean (Vigna unguilata L). Treatments were P rate : 0, 45, 90, 135 dan 180 kg P2O5ha-1 or equal to 0...

  9. Phosphorus correlation with relative Yield of Vegetable Grown in the Ultisol-Nanggung, Bogor, Indonesia

    OpenAIRE

    Kartika, J.G.; Anas D Susila

    2007-01-01

    Phosphorus (P) correlation experiment has been done in Ultisol soil in Nanggung District, Bogor, Indonesia from 2006-2007 to find the best P extraction method which is suitable for vegetables i.e Amaranth (Amaranthus sp), Kangkong (Ipomoea aquatica L), Eggplant (Solanum melongena L), Chili (Capsicum annuum L), Tomato (Lycopersicon esculentum Mill), Green Bean (Pahaseolus vulgaris L), and Yard Long Bean (Vigna unguilata L). Treatments were P rate : 0, 45, 90, 135 and 180 kg P2O5ha-1 or equal t...

  10. The anthocyanidin synthase gene from sweetpotato [Ipomoea ...

    African Journals Online (AJOL)

    USER

    2010-06-21

    Jun 21, 2010 ... potato (Ipomoea batatas (L.) Lam) Yuzi 263, which was designated as IbANS. ... The higher expression level of IbANS was found in diameter (3.0 cm) of .... phosphate buffer (pH 3.0) and then added to the final volume of 50.

  11. Occurrence of white rust (Albugo ipomoeae-panduratae on Ipomoea acuminata in the brazilian mid-west

    Directory of Open Access Journals (Sweden)

    A.P.S Pagani

    2012-03-01

    Full Text Available Spontaneous plants of Ipomoea acuminata ("morning glory" exhibiting white rust pustules were found in a field crop area of Planaltina, DF, in the fall season of 2010 and the disease causal agent was identified as Albugo ipomoea-panduratae (Oomycota. No reports of the association between A. ipomoea-panduratae and I. acuminata were known in Brazil previously to 2010. A reference specimen was deposited at the University of Brasilia Mycological Reference Collection.

  12. Pinoresinol from Ipomoea cairica cell cultures.

    Science.gov (United States)

    Páska, Csilla; Innocenti, Gabbriella; Ferlin, Mariagrazia; Kunvári, Mónika; László, Miklós

    2002-10-01

    Ipomoea cairica cell cultures produced a tetrahydrofuran lignan, (+)-pinoresinol, identified by UV, IR, MS and NMR methods, not yet found in the intact plant, and new in the Convolvulaceae family. Pinoresinol was found to have antioxidant and Ca2+ antagonist properties. As it could be requested for its biological activity, we examined the possibility to raise the pinoresinol yield of I. cairica cultures, as well as we continued investigations on lignans' response to optimization.

  13. Water Spinach, Ipomoea aquatic (Convolvulaceae, Ameliorates Lead Toxicity by Inhibiting Oxidative Stress and Apoptosis.

    Directory of Open Access Journals (Sweden)

    Saikat Dewanjee

    Full Text Available Ipomoea aquatica (Convolvulaceae, an aquatic edible plant, is traditionally used against heavy metal toxicity in India. The current study intended to explore the protective role of edible (aqueous extract of I. aquatica (AEIA against experimentally induced Pb-intoxication.The cytoprotective role of AEIA was measured on mouse hepatocytes by cell viability assay followed by Hoechst staining and flow cytometric assay. The effect on ROS production, lipid peroxidation, protein carbonylation, intracellular redox status were measured after incubating the hepatocytes with Pb-acetate (6.8 μM along with AEIA (400 μg/ml. The effects on the expressions of apoptotic signal proteins were estimated by western blotting. The protective role of AEIA was measured by in vivo assay in mice. Haematological, serum biochemical, tissue redox status, Pb bioaccumulation and histological parameters were evaluated to estimate the protective role of AEIA (100 mg/kg against Pb-acetate (5 mg/kg intoxication.Pb-acetate treated hepatocytes showed a gradual reduction of cell viability dose-dependently with an IC50 value of 6.8 μM. Pb-acetate treated hepatocytes exhibited significantly enhanced levels (p < 0.01 of ROS production, lipid peroxidation, protein carbonylation with concomitant depletion (p < 0.01 of antioxidant enzymes and GSH. However, AEIA treatment could significantly restore the aforementioned parameters in murine hepatocytes near to normalcy. Besides, AEIA significantly reversed (p < 0.05-0.01 the alterations of transcription levels of apoptotic proteins viz. Bcl 2, Bad, Cyt C, Apaf-1, cleaved caspases [caspase 3, caspase 8 and caspase 9], Fas and Bid. In in vivo bioassay, Pb-acetate treatment caused significantly high intracellular Pb burden and oxidative pressure in the kidney, liver, heart, brain and testes in mice. In addition, the haematological and serum biochemical factors were changed significantly in Pb-acetate-treated animals. AEIA treatment restored

  14. Phytophthora ipomoeae, a new homothallic species causing leaf blight on Ipomoea longipedunculata in the Toluca Valley of central Mexico

    NARCIS (Netherlands)

    Flier, W.G.; Grunwald, N.J.; Kroon, L.P.N.M.; Bosch, van den G.B.M.; Garay-Serrano, E.; Lozoya-Saldan, H.; Bonants, P.J.M.; Turkensteen, L.J.

    2002-01-01

    A Phytophthora species was found on blighted foliage of Ipomoea longipedunculata, a morning glory native to the highlands of central Mexico. Based on host range, morphology, allozymes, mitochondrial DNA haplotype and rDNA sequences it is concluded that a new Phytophthora species, P. ipomoeae sp. nov

  15. Comparative Foliar Epidermal Studies in Coix lacryma-jobi L. andCoix aquatica Roxb. (Poaceae

    Directory of Open Access Journals (Sweden)

    Rinku Jitendrakumar DESAI

    2009-12-01

    Full Text Available As micromorphological knowledge was not available for Coix aquatica Roxb., the foliar epidermal studies were carried out for Coix lacryma-jobi L. and Coix aquatica Roxb. with the aim of determining the patterns of variation in their epidermal characteristics and assessing their value in species identification. Comparative foliar analysis was carried out by using light microscopy, after following routine scraping method. The characters of diagnostic importance in the identification of C. aquatica are the sparsely distributed prickle hairs with long pointed apex in the abaxial epidermis and dumbbell shaped silica cells in both the epidermises. The diagnostic characters for C. lacryma-jobi are the cross shaped silica cells and dumbbell shaped on the abaxial and adaxial epidermis respectively. The observed differences in certain micromorphological characters helps in identification of presently studied two species of Coix.

  16. Ecologia floral de duas especies invasoras de Ipomoea (convolvulaceae)

    OpenAIRE

    Adriana de Oliveira Fidalgo

    1997-01-01

    Resumo: Aspectos do sistema de reprodução, morfologia e biologia florais e a ocorrência de ataque de herbívoros às flores de Ipomoea cairica (L.) Sweet e Ipomoea carnea ssp. fistu/osa (Martius ex Choisy) Austin (Convolvulaceae) foram estudados no Município de Campinas/São Paulo, Brasil, entre julho de 1995 e julho de 1996. Ipomoea cairica é uma planta invasora de culturas, herbácea com ramos longos e volúveis, capaz de se reproduzir por sementes e vegetatívamente. Suas flores são inodoras, tu...

  17. Effect of Alisma plantago-aquatica Linn extract on chronic prostatitis ...

    African Journals Online (AJOL)

    Purpose: To study the effect of Alisma plantago-aquatica Linn. extract (APLE) on ... Results: A high dose of APLE (480 mg/kg) significantly decreased PI (0.7 ... Index Medicus, JournalSeek, Journal Citation Reports/Science Edition, ..... This is an Open Access article that uses a fund- .... Jiang CY, Wang W, Tang JX, Yuan ZR.

  18. Phage Tail-Like (High-Molecular-Weight) Bacteriocins of Budvicia aquatica and Pragia fontium (Enterobacteriaceae)

    OpenAIRE

    Šmarda, Jan; Benada, Oldřich

    2005-01-01

    Electron microscopic analysis of contractile phage tail-like bacteriocins of three Pragia fontium strains and one Budvicia aquatica strain was performed. Fonticin and aquaticin are remarkably heat sensitive but trypsin resistant. Simultaneous production of contractile and flexible phage tail-like bacteriocins in the P. fontium 64613 strain is shown for the first time.

  19. Novel begomovirus species of recombinant nature in sweet potato (Ipomoea batatas) and Ipomoea indica: taxonomic and phylogenetic implications.

    Science.gov (United States)

    Lozano, Gloria; Trenado, Helena P; Valverde, Rodrigo A; Navas-Castillo, Jesús

    2009-10-01

    Viral diseases occur wherever sweet potato (Ipomoea batatas) is cultivated and because this crop is vegetatively propagated, accumulation and perpetuation of viruses can become a major constraint for production. Up to 90% reductions in yield have been reported in association with viral infections. About 20 officially accepted or tentative virus species have been found in sweet potato and other Ipomoea species. They include three species of begomoviruses (genus Begomovirus, family Geminiviridae) whose genomes have been fully sequenced. In this investigation, we conducted a search for begomoviruses infecting sweet potato and Ipomoea indica in Spain and characterized the complete genome of 15 isolates. In addition to sweet potato leaf curl virus (SPLCV) and Ipomoea yellowing vein virus, we identified three new begomovirus species and a novel strain of SPLCV. Our analysis also demonstrated that extensive recombination events have shaped the populations of Ipomoea-infecting begomoviruses in Spain. The increased complexity of the unique Ipomoea-infecting begomovirus group, highlighted by our results, open new horizons to understand the phylogeny and evolution of the family Geminiviridae.

  20. Microstructure of the water spider (Argyroneta aquatica using the scanning electron microscope

    Directory of Open Access Journals (Sweden)

    Jung-Hoon Kang

    2014-12-01

    Full Text Available This study is aimed to identify the external features of the water spiders (Argyroneta aquatica collected from “The Natural Monument No. 412 Yeoncheon Eundaeri Water Spider Habitat” through observation of their microstructures using a scanning electron microscope. There is no study on the microstructures of the water spiders excluding several studies on protection plans and ecological investigations, thus giving this study considerable academic significance. Based on the scanning electron microscopy analysis, the water spider has eight simple eyes, and both of its lateral simple eyes are stuck together. A lateral bump was confirmed on the upper jaw, and the pedipalps had six joints and the legs had seven joints. The abdomen and sternum of A. aquatica have more hairs compared with those of land spiders, and its structure shows an elongated area of contact with the air bell so that the air bell can become attached to the abdomen better.

  1. Coliform bacteria removal from sewage in constructed wetlands planted with Mentha aquatica.

    Science.gov (United States)

    Avelar, Fabiana F; de Matos, Antonio T; de Matos, Mateus P; Borges, Alisson C

    2014-08-01

    The present study evaluated the performance of the species Mentha aquatica in constructed wetlands of horizontal subsurface flow (CW-HSSF) with regard to the removal of coliforms bacteria in an effluent from the primary treatment of sewage as well as to obtain adjustment parameters of the bacterial decay kinetic model along the length of the CW-HSSF. Therefore, four CW-HSSFs measuring 24.0 m x 1.0 m x 0.35 m were built and filled with number 0 gravel as the support medium to a height of 0.20m. Two of the CW-HSSFs were planted with the species M. aquatica, while the other two remained uncultivated. Cultivation of M. aquatica in CW-HSSF resulted in total coliforms (TC) and Escherichia coli (EC) removals from 0.9 to 1.3 log units greater than those obtained in the uncultivated experimental plots, for the hydraulic retention times (HRTs) of 4.5 and 6.0 days. For HRT ranged from 1.5 to 6.0 days, the highest removal efficiencies in counts of TC and EC were obtained when using longer HRT. The mathematical models evaluated showed good fit to average counts of TC and EC highlighting the modified first-order kinetic model with the inclusion of the power parameter in the HRT variable.

  2. Effect of Rotula aquatica Lour. on ethylene-glycol induced urolithiasis in rats.

    Directory of Open Access Journals (Sweden)

    Christina A.J.M

    2011-03-01

    Full Text Available The effect of the alcoholic extract of Rotula aquatica (Boraginaceae against ethylene glycolinduced urolithiasis in albino rats is summarized in this study. Lithiasis was induced in rats by administrating 1% ethylene glycol in drinking water for 28 days and was manifested by high urinary calcium, phosphate, oxalate, protein, uric acid, creatinine and low urinary magnesium content. The alcoholic extract of Rotula aquatica was administered in 200 mg/kg body weight orally for 28 days along with 1% ethylene glycol. Urinary calcium, phosphate, oxalate, protein, uric acid, creatinine was reduced and urinary magnesium level was elevated. It also increased the urine volume, thereby reducing the tendency for crystallization. The histopathological studies confirmed the induction of lithiasis as microcrystal deposition was observed in section of kidney from animals treated with ethylene glycol. This was reduced, however, after treatment with the extract. These observations enable us to conclude that alcoholic extract of Rotula aquatica is effective against ethylene-glycol induced urolithiasis in albino rats.

  3. Experimental swainsonine poisoning in goats ingesting Ipomoea sericophylla and Ipomoea riedelii (Convolvulaceae Intoxicação experimental por swainsonina em caprinos ingerindo Ipomoea sericophylla e Ipomoea riedelii

    Directory of Open Access Journals (Sweden)

    Rossemberg C. Barbosa

    2007-10-01

    Full Text Available Ipomoea sericophylla and Ipomoea riedelii cause a glycoprotein storage disease in goats. This paper reports the experimental poisoning in goats by dried I. sericophylla and I. riedelii containing 0.05% and 0.01% swainsonine, respectively. Three groups with four animals each were used. Group 1 received daily doses of 2g/kg body weight (bw of dried I. sericophylla (150mg of swainsonine/kg. Goats from this group had clinical signs 36-38 days after the start of ingestion. Group 2 received dried I. riedelii daily doses of 2g/kg of I. riedelii (30mg of swainsonine/kg for 70 days. No clinical signs were observed, therefore the swainsonine dose was increased to 60mg/kg for another 70 days. Goats from Group 2 had clinical signs 26-65 days after increase in swainsonine dose to 60mg/kg. Group 3 was used as control. In these experiments the minimum toxic dose was 60mg/kg which represents 0.0004% of the dry matter in goats ingesting 1.5% bw of the dry matter. For goats ingesting 2%-2.5% bw of dry matter this dose would be 0.00024%-0.0003% of the dry matter. After the end of the experiment two goats were euthanized and another six were observed for recovery of clinical signs. Four goats that continued to consume swainsonine containing plant for 39-89 days after the first clinical signs had non reversible signs, while two goats that ingested the plant for only 15 and 20 days after the first clinical signs recovered completely. These and previous results indicate that irreversible lesions due to neuronal loss occur in goats that continue to ingest the plants for about 30 days after the first clinical signs. Clinical signs and histological lesions were similar to those reported previously for goats poisoned by swainsonine containing plants. No significant alterations were found in packed cell volume, red and white blood cell counts, hemoglobin and mean corpuscular hemoglobin concentrations, mean corpuscular volume, and serum levels of glucose, total protein, and

  4. High-yield cultivation techniques of large-leaved water spinach(Ipomoea aquatica Forsk)%大叶空心菜高产栽培技术

    Institute of Scientific and Technical Information of China (English)

    叶敏

    2015-01-01

    总结大叶空心菜高产栽培技术,主要包括播种育苗、定植、田间管理、病虫害防治、采收等方面内容。%High-yield cultivation techniques of large-leaved water spinach was summarized,mainly including culture of see-ding,field planting,field management,control of diseases and pests,harvesting and other aspects.

  5. Elimination of the tremorgenic toxin of Ipomoea asarifolia by milk

    Science.gov (United States)

    With the aim to determine if the tremorgenic toxin of Ipomoea asarifolia is eliminated in milk, three groups of Swiss female mice received, immediately after giving birth until weaning, a ration containing 20% or 30% of dry I. asarifolia. All the offspring of the females that received 20% or 30% I. ...

  6. Characterization of pentasaccharide glycosides from the roots of Ipomoea arborescens.

    Science.gov (United States)

    León, Ismael; Mirón, Gumersindo; Alonso, Daniel

    2006-06-01

    Ten new pentasaccharide glycosides, arboresins 1-6 (1-6) and murucins 6-9 (8-11), along with five known glycolipids, were isolated from the roots of Ipomoea arborescens, and their structures were elucidated by spectroscopic and chemical methods. Compounds 1-6 and 8-11 were evaluated for cytotoxicity against a small panel of cancer cell lines.

  7. Recovery of methane-rich gas from solid-feed anaerobic digestion of ipomoea (Ipomoea carnea).

    Science.gov (United States)

    Sankar Ganesh, P; Sanjeevi, R; Gajalakshmi, S; Ramasamy, E V; Abbasi, S A

    2008-03-01

    Studies are presented on new types of anaerobic digesters in which chopped or dry crushed Ipomoea carnea was fed without any other pretreatment, in an attempt to develop commercially viable means of utilizing the otherwise very harmful plant. Two types of solid-feed anaerobic digesters (SFADs) were studied. The first type had a single vessel in which the bottom 35% portion was separated from the top portion by a perforated PVC disk. The weed was charged from the top and inoculated with anaerobically digested cowdung-water slurry. The fermentation of the weed in the reactor led to the formation of volatile fatty acids (VFAs) plus some biogas. The leachate, rich in the VFAs, was passed through the perforated PVC sheet and collected in the lower portion of the vessel. The other type of reactors had two vessels, the first one was fully charged with the weed and the second received the VFA leachate. With both types were attached upflow anaerobic filters (UAFs) which converted the leachate into combustible biogas consisting of approximately 70% methane. All SFADs developed very consistent performance in terms of biogas yield within 17 weeks of start. The two-compartment reactors yielded significantly more biogas than the single-compartment reactors of corresponding total volume, and the reactors with which anaerobic filters (AF) were attached yielded more biogas than the ones without AF. The best performing units generated 2.41m(3) of biogas per m(3) of digester volume, as compared to 0.1-0.2m(3) of biogas, m(-3)d(-1), obtainable with conventional digesters. This indicates the viability of this technology. The spent weed can be vermicomposted directly to obtain good soil-conditioner cum fertilizer; earthworm Eudrilus eugeniae produced 540mg vermicast per animal every day, achieving near total conversion of feed to vermicast in 20 days. The proposed systems, thus, makes it possible to accomplish total utilization of ipomoea.

  8. Anti-ulcer activity of Ipomoea batatas tubers (sweet potato

    Directory of Open Access Journals (Sweden)

    Vandana Panda

    2012-04-01

    Full Text Available Background: Peptic ulcers occur in that part of the gastrointestinal tract which is exposed to gastric acid and pepsin, i.e., the stomach and duodenum. Gastric and duodenal ulcers are common pathologies that may be induced by a variety of factors such as stress, smoking and noxious agents including non-steroidal anti-inflammatory drugs. Ipomoea batatas tubers (sweet potato contain ample amounts of antioxidants. It has been proven already by many scientific studies that antioxidants have ulcer healing properties. In reference to this, we tried assessing the ulcer healing effect of Ipomoea batatas tubers. Methods: The anti-ulcer activity of the tubers of Ipomoea batatas (sweet potato was studied in cold stress and aspirin-induced gastric ulcers in Wistar rats. Methanolic extracts of Ipomoea batatas tubers (TE at two doses, viz., 400 and 800 mg /kg were evaluated in cold stress and aspirin-induced gastric ulcer models using cimetidine and omeprazole respectively as standards. The standard drugs and the test drugs were administered orally for 7 days in the cold stressmodel and for 1 day in the aspirin-induced gastric ulcer model. Gastroprotective potential, status of the antioxidant enzymes {superoxide dismutase (SOD, catalase (CAT, glutathione peroxidase (GPx and glutathione reductase(GR} along with GSH, and lipid peroxidation were studied in both models. Results: The results of the present study showed that TE possessed gastroprotective activity as evidenced by its significant inhibition of mean ulcer score and ulcer index and a marked increase in GSH, SOD, CAT, GPx, and GR levels and reduction in lipid peroxidation in a dose dependant manner.Conclusion: The present experimental findings suggest that tubers of Ipomoea batatas may be useful for treating peptic ulcers.

  9. Ecological preference of the diving bell spider Argyroneta aquatica in a resurgence of the Po plain (Northern Italy (Araneae: Cybaeidae

    Directory of Open Access Journals (Sweden)

    Stefano Mammola

    2016-06-01

    Full Text Available The diving bell spider Argyroneta aquatica is the only known spider to conduct a wholly aquatic life. For this reason, it has been the object of an array of studies concerning different aspects of its peculiar biology such as reproductive behavior and sexual dimorphism, physiology, genetic and silk. On the other hand, besides some empirical observations, the autoecology of this spider is widely understudied. We conducted an ecological study in a resurgence located in the Po Plain (Northern Italy, Province of Vercelli hosting a relatively rich population of Argyroneta aquatica, aiming at identifying the ecological factors driving its presence at the micro-habitat level. By means of a specific sampling methodology, we acquired distributional data of the spiders in the study area and monitored physical-chemical and habitat structure parameters at each plot. We analyzed the data through Bernoulli Generalized Linear Models (GLM. Results pointed out a significant positive effect of the presence of aquatic vegetation in the plot. In addition, the presence of A. aquatica was significantly associated with areas of the resurgence characterized at the same time by high prey availability and low density of predators. Considering the ecological importance and rarity of this species, we update and revise the data on the distribution of A. aquatica in Italy.

  10. Variation in light absorption properties of mentha aquatica L. as a function of leaf form: Implications for plant growth

    DEFF Research Database (Denmark)

    Enriquez, Susana; Jensen, Kaj Sand

    2008-01-01

    area increased. This relationship indicates that dispersive samples, such as leaves, although efficient light traps, can also be affected by the "package effect." Mentha aquatica leaves, by expanding their biomass (increased specific leaf area [SLA]), improve their light absorption efficiency per unit...

  11. Secretory structures of Ipomoea asarifolia: anatomy and histochemistry

    OpenAIRE

    Martins,Fabiano M.; Lima,Jamile F.; Ana Angélica S. Mascarenhas; Thayane P. Macedo

    2012-01-01

    Ipomoea asarifolia (Desr.) Roem. & Schult., Convolvulaceae, is a weed that infests agricultural areas and is toxic to cattle. In spite of its toxicity, the leaves of this plant are used in traditional remedies in the state of Bahia, Brazil. The present work describes the leaf anatomy of I. asarifolia and characterizes the exudates of its secretory structures. The leaves have a unistratified epidermis composed of ordinary cells with straight to slightly sinuous anticlinal walls and thin cu...

  12. Control of amphibious weed ipomoea (Ipomoea carnea by utilizing it for the extraction of volatile fatty acids as energy precursors

    Directory of Open Access Journals (Sweden)

    M. Rafiq Kumar

    2015-01-01

    Full Text Available Volatile fatty acids (VFAs, comprising mainly of acetic acid and lesser quantities of propionic and butyric acids, are generated when zoomass or phytomass is acted upon by acidogenic and acetogenic microorganisms. VFAs can be utilized by methanogens under anaerobic conditions to generate flammable methane–carbon dioxide mixtures known as ‘biogas’. Acting on the premise that this manner of VFA utilization for generating relatively clean energy can be easily accomplished in a controlled fashion in conventional biogas plants as well as higher-rate anaerobic digesters, we have carried out studies aimed to generate VFAs from the pernicious weed ipomoea (Ipomoea carnea. The VFA extraction was accomplished by a simple yet effective technology, appropriate for use even by laypersons. For this acid-phase reactors were set, to which measured quantities of ipomoea leaves were charged along with water inoculated with cow dung. The reactors were stirred intermittently. It was found that VFA production started within hours of the mixing of the reactants and peaked by the 10th or 11th day in all the reactors, effecting a conversion of over 10% of the biomass into VFAs. The reactor performance had good reproducibility and the process appeared easily controllable, frugal and robust.

  13. Ameliorative effect of water spinach, Ipomea aquatica (Convolvulaceae), against experimentally induced arsenic toxicity.

    Science.gov (United States)

    Dua, Tarun K; Dewanjee, Saikat; Gangopadhyay, Moumita; Khanra, Ritu; Zia-Ul-Haq, Muhammad; De Feo, Vincenzo

    2015-03-05

    Ipomea aquatica (Convolvulaceae) is traditionally used against Arsenic (As) poisoning in folk medicines in India. The present study was designed to explore the therapeutic role of aqueous extract of I. aquatica (AEIA) against As-intoxication. AEIA was chemically standardized by spectroscopic and chromatographic analysis. The cytoprotective role of AEIA was measured on isolated murine hepatocytes. The effect on redox status were measured after incubating the hepatocytes with NaAsO2 (10 μM) + AEIA (400 μg/ml). The protective effect of AEIA (400 μg/ml) in expressions of apoptotic proteins were estimated in vitro. The protective role of AEIA was measured by in vivo assay in mice. Haematological, biochemical, As bioaccumulation and histological parameters were evaluated to ensure the protective role of AEIA (100 mg/kg) against NaAsO2 (10 mg/kg) intoxication. Phytochemical analysis revealed presence of substantial quantities of phenolics, flavonoids, saponins and ascorbic acid in AEIA. Incubation of murine hepatocytes with AEIA (0-400 μg/ml) + NaAsO2 (10 μM) exerted a concentration dependent cytoprotective effect. Incubation of murine hepatocytes with NaAsO2 (10 μM, ~ IC50) induced apoptosis via augmenting oxidative stress. NaAsO2 treated hepatocytes exhibited significantly (p animals. However, concurrent administration of AEIA (100 mg/ml) could significantly reinstate the NaAsO2-induced pathogenesis. Presence of substantial quantities of dietary antioxidants within AEIA would be responsible for overall protective effect.

  14. Estaquia de Ipomoea cairica (L. Sweet Stem cutting of Ipomoea cairica (L. Sweet

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    G.B. Tognon

    2012-01-01

    Full Text Available A Ipomoea cairica (L. Sweet é uma trepadeira herbácea nativa, amplamente utilizada na medicina popular brasileira e inexistem informações sobre a propagação vegetativa. Em vista disso, objetivou-se avaliar a presença de folhas nas estacas, o substrato e a dose de reguladores vegetais mais adequados para a propagação por estaquia. Foram conduzidos dois experimentos, no primeiro, foram avaliados dois tipos de estacas (com e sem folhas e quatro substratos (areia, casca de arroz carbonizada, latossolo vermelho distrófico + matéria orgânica - 1:1, latossolo vermelho distrófico + matéria orgânica + areia - 1:1:2, enquanto no segundo foram testadas cinco concentrações diferentes de AIB (0, 250, 500, 750 e 1000 mg L-1. Após 20 dias, obteve-se 86,2% de estacas enraizadas no substrato areia e, no segundo experimento, observou-se que o AIB não influenciou a porcentagem de enraizamento (94% em média. Conclui-se que a presença de folhas melhora a qualidade do enraizamento, que os substratos indicados para a propagação são areia e casca de arroz carbonizada devido ao maior enraizamento, fácil disponibilidade e baixo custo, e que a utilização de AIB na concentração 250 mg L-1 é a mais adequada para propagação por estaquia de I. cairica.Ipomoea cairica (L. Sweet is an herbaceous climbing plant widely used in Brazilian folk medicine and there is no information regarding its vegetative propagation. In view of this, the aim of this study was to evaluate the presence of leaves on stem cuttings and the most adequate substrate and level of plant growth regulators for propagation by stem cuttings of this morning glory. Two experiments were conducted, in the first, two types of stem cuttings (with and without leaves and four substrates (sand; carbonized rice hull; Haplortox + organic matter - 1:1; Haplortox + organic matter + sand - 1:1:2 were evaluated, and in the second experiment five different concentrations of IBA (0, 250, 500, 750

  15. Ipangulines and minalobines, chemotaxonomic markers of the infrageneric Ipomoea taxon subgenus Quamoclit, section Mina.

    Science.gov (United States)

    Jenett-Siems, Kristina; Ott, Sonja C; Schimming, Thomas; Siems, Karsten; Müller, Frank; Hilker, Monika; Witte, Ludger; Hartmann, Thomas; Austin, Daniel F; Eich, Eckart

    2005-01-01

    A comprehensive GC-MS analysis of 8 Ipomoea species belonging to the subgenus Quamoclit, section Mina revealed that the members of this taxon form combinations of two necine bases with rare necic acids resulting in unique pyrrolizidine alkaloids. The occurrence and diversity of these metabolites show remarkable variations: Some species, especially Ipomoea hederifolia and Ipomoea lobata, are able to synthesize a large number of alkaloids whereas others, especially Ipomoea coccinea and Ipomoea quamoclit, are poor synthesizers with only a few compounds. However, these metabolites are apparently chemotaxonomic markers of this infrageneric taxon in general. They represent either esters of (-)-platynecine (altogether 48 ipangulines and 4 further esters including results of a previous study) or esters of (-)-trachelanthamidine, an additional novel structural type called minalobines (altogether 21 alkaloids). Both types are characterized by section-specific rare necic acids, e.g., ipangulinic/isoipangulinic acid, phenylacetic acid. The alkaloids of Ipomoea cholulensis, I. coccinea, I. hederifolia, Ipomoea neei, and Ipomoea quamoclit were mono and diesters of platynecine. Minalobines turned out to be metabolites of I. lobata (Cerv.) Thell. (syn.: Mina lobata Cerv.) lacking ipangulines. The major alkaloid of this species, minalobine R, has been isolated and identified as 9-O-(threo-2-hydroxy-2-methyl-3-phenylacetoxy-butyryl)-(-)-trachelanthamidine on the basis of spectral data. Apparently only two of the species included in this study, Ipomoea cristulata and Ipomoea sloteri, are able to synthesize both, ipangulines as well as minalobines. Minalobine O could be isolated as a major alkaloid of I. cristulata, its structure has been established as 9-O-(erythro-2-hydroxy-2-methyl-3-tigloyloxy-butyryl)-(-)-trachelanthamidine on the basis of spectral data.

  16. Caracterização do óleo das sementes de Pachira aquatica Aublet para aproveitamento alimentar Characterization of seed oil Pachira aquatica Aublet for food utilization

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    Neuza Jorge

    2012-03-01

    Full Text Available Os frutos de Pachira aquatica Aublet apresentam sementes comestíveis com características organolépticas muito apreciadas pelas populações amazônicas, sendo pouco utilizados em outras regiões. Este trabalho teve como objetivo caracterizar as sementes quanto à composição centesimal e determinar as características físico-químicas e perfil de ácidos graxos. A determinação da composição centesimal das sementes (teores de umidade, lipídios, proteínas, cinzas e carboidratos e análises do óleo extraído das mesmas (ácidos graxos livres, índices de peróxido, iodo, refração, saponificação, ponto de fusão e perfil de ácidos graxos foram realizadas seguindo metodologia oficial. O teor de óleo nas sementes 38,39% demonstrou que estas têm potencial para aproveitamento industrial. Das características físico-químicas analisadas, o óleo extraído das sementes apresentou 39,2% de ácidos graxos livres (expresso em % ácido oleico, índice de iodo de 27,4 g I2.100 g-1, índice de saponificação de 208,0 mg.KOH g-1, índice de refração (40 °C de 1,4569 e ponto de fusão de 41,9 °C. Quanto à composição de ácidos graxos do óleo predominaram os ácidos palmítico (44,93%, oleico (39,27% e linoleico (11,35%. Tal fato favorece o uso deste óleo como matéria-prima para as indústrias alimentícia, farmacêutica e de cosméticos.The fruits of Pachira aquatica Aublet present edible seeds with organoleptic characteristics much appreciated by peoples in the Amazon, with little being used in other regions. This study aimed to characterize the seeds and to determine the composition and physicochemical characteristics and fatty acid profile. Determining the composition of the seeds (moisture, lipids, proteins, carbohydrates and ash and analysis of oil extracted from them (free fatty acids, peroxide, iodine, refractive index, saponification value, melting point and fatty acid profile was performed following the official methodology

  17. Pentasaccharide resin glycosides from Ipomoea cairica and their cytotoxic activities.

    Science.gov (United States)

    Yu, Bangwei; Luo, Jianguang; Wang, Junsong; Zhang, Dongming; Yu, Shishan; Kong, Lingyi

    2013-11-01

    Six partially acylated pentasaccharide resin glycosides, cairicosides A-F, were isolated from the aerial parts of Ipomoea cairica. These compounds were characterized as a group of macrolactones of simonic acid A, partially acylated with different organic acids. The lactonization site of 11S-hydroxyhexadecanoic acid (jalapinolic acid) was bound to the second saccharide moiety at C-3 in cairicosides A-E, while at C-2 in cairicoside F. Structures were established by spectroscopic and chemical methods. Compounds cairicosides A-E exhibited moderate cytotoxicity against a small panel of human tumor cell lines with IC50 values in the range of 4.28-14.31μM.

  18. Anti-Inflammatory Activity of Ipomoea reniformis Methanolic Extract

    OpenAIRE

    Sanja S. D.; Sheth N.R.; Joshi D. M.; Golwala D.K.; Patel Dhaval; Raval M. K.

    2009-01-01

    In the present study, methanolic extract of Ipomoea reniformis herb (MEIR) in acute, subacute and chronic models of inflammation was assessed in rats. Administration of MEIR (200, 400 mg/kg, p.o.) exhibited significant anti-inflammatory activity. In acute inflammation as produced by Carrageenan 59.55 % and 64.04 % protection was observed. While in subacute anti-inflammatory models using formaldehyde-induced hind paw edema (after 1.5 h) 38.36 % and 47.95 % and in chronic anti-inflammatory mode...

  19. Structural investigation of resin glycosides from Ipomoea lonchophylla.

    Science.gov (United States)

    MacLeod, J K; Ward, A; Oelrichs, P B

    1997-05-01

    A fraction from Ipomoea lonchophylla, which was toxic to mice, contained an inseparable mixture of resin glycosides with differing numbers of C5 ester groups on the hexasaccharide chain. After alkaline hydrolysis of the esters, the structure of the major component (1) was elucidated using high-field NMR spectroscopy, mass spectrometry, chemical studies, and comparison with known resin glycosides. Compound 1 was identified as 3,11-dihydroxytetradecanoic acid 11-O-beta-quinovopyranosyl-(1-->2)-beta-glucopyranosyl-(1-->3)- [alpha-rhamnopyranosyl- (1-->4)]-quinovopyranosyl-(1-->2)-beta-glucopyranosyl-(1-->2)-beta -fucopyranoside.

  20. O Gênero Ipomoea L.(Convolvulaceae) no Rio Grande do Sul

    OpenAIRE

    Priscila Porto Alegre Ferreira

    2009-01-01

    A família Convolvulaceae possui 55 gêneros e 1600-1700 representantes com distribuição cosmopolita. Ipomoea é o gênero com maior riqueza específica, 600-700 táxons, e está representado no Brasil por aproximadamente 140 espécies. O presente estudo trata dos táxons de Ipomoea ocorrentes no Rio Grande do Sul. Foram reconhecidas 29 espécies para o Estado: Ipomoea acutisepala O’Donell, I. alba L., I. bonariensis Hook., I. cairica (L.) Sweet, I. delphinioides Choisy, I. descolei O’Donell, I. fimbri...

  1. Anti-Inflammatory Activity of Ipomoea reniformis Methanolic Extract

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    Sanja S. D.

    2009-10-01

    Full Text Available In the present study, methanolic extract of Ipomoea reniformis herb (MEIR in acute, subacute and chronic models of inflammation was assessed in rats. Administration of MEIR (200, 400 mg/kg, p.o. exhibited significant anti-inflammatory activity. In acute inflammation as produced by Carrageenan 59.55 % and 64.04 % protection was observed. While in subacute anti-inflammatory models using formaldehyde-induced hind paw edema (after 1.5 h 38.36 % and 47.95 % and in chronic anti-inflammatory model using cotton pellet granuloma 15.02 % and 19.19 % protection from inflammation was observed. MEIR did not show any sign of toxicity and mortality up to a dose level of 1000 mg/kg, p.o. in rats. The results obtained suggest that the methanolic extract of Ipomoea reniformis herb (MEIR is endowed with effective anti-inflammatory activity mediated via either by inhibition of cyclooxygenase cascade and by blocking the release of vasoactive substances (histamine, serotonin and kinins. These findings seem to justify the use of the plant in traditional Indian medicine in the treatment of inflammation, including arthritic conditions.

  2. Growth inhibition of struvite crystals by the aqueous root extract of Rotula aquatica.

    Science.gov (United States)

    Chauhan, C K; Joshi, M J; Vaidya, A D B

    2011-06-01

    Formation of urinary stone is a serious and debilitating problem throughout the world. In the present study, the inhibitory effect of aqueous extract of root of Rotula aquatica was investigated against struvite crystals (one of the components of urinary stone) grown in vitro using single diffusion gel growth technique. For setting the gel, sodium metasilicate solution (specific gravity 1.05) and 0.5 M aqueous solution of ammonium dihydrogen phosphate were mixed, so that the pH of the mixture could be set at 7.0. Equal amounts of supernatant solution of magnesium acetate (1.0 M) prepared with 0.0%, 0.5% and 1% concentrations of the extract were gently poured on the set gels. It was observed that the number, dimension, total mass, total volume, growth rate and depth of growth of struvite crystals decreased with the increasing extract concentrations in the supernatant solutions. The enhancement of dissolution rate and fragmentation of struvite crystals suggested potential application of the extract for inhibition of struvite type urinary stone.

  3. Morphological characterization of fruits, seeds and phases of germination Pachira aquatica Aubl. (Bombacaceae Caracterização morfológica de frutos, sementes e fases da germinação de Pachira aquatica Aubl. (Bombacaceae

    Directory of Open Access Journals (Sweden)

    Riselane de Lucena Alcântara Bruno

    2012-06-01

    Full Text Available The mongubeira (Pachira aquatica Aubl is a fruit used in urban forestry in many cities within the country and infrequent in their natural habitat in the forests periodically flooded the coast of Pará and Maranhão. This study aimed to describe and illustrate the external and internal morphological characteristics of fruits and seeds, as well as the stages of germination of P. aquatica. For a description of the fruits were observed internal and external details of the pericarp, related to texture, texture, hairiness, brightness, shape, number of seeds per fruit and dehiscence. For seed morphology were observed following external dimensions, color, texture, consistency, shape, position of hilum and micropyle. For the internal characteristics, there was the presence or absence of endosperm and embryo type. The fruits of P. aquatica capsules are semi-woody, dehiscent, generally oval shape, some are curved and longitudinal cracks in the pericarp, is made up of five septa, polispermic and stained dark brown. The seeds vary from angular to reniform, have a large amount of endosperm and are inserted into the central axis of the fruit. Germination is epigeal type phanerocotylar, starting on the eighth day and can be terminated on the 15th day after sowing.A mongubeira (Parchira aquatica Aubl. é uma frutífera utilizada na arborização urbana de muitas cidades no interior do país e pouco frequente em seu habitat natural, nas matas periodicamente inundáveis do litoral do Pará e Maranhão. Este trabalho teve como objetivo descrever e ilustrar as características morfológicas externas e internas de frutos e sementes, bem como as fases do processo de germinação de P. aquatica. Para a descrição dos frutos foram observados detalhes externos e internos do pericarpo, referentes à textura, consistência, pilosidade, brilho, forma, número de sementes por fruto e deiscência. Para a morfologia de sementes foram observadas as seguintes caracter

  4. A review on Ipomoea carnea: pharmacology, toxicology and phytochemistry.

    Science.gov (United States)

    Fatima, Nusrat; Rahman, Mohammad Mijanur; Khan, Md Asaduzzaman; Fu, Junjiang

    2014-06-01

    Phytomedicines are increasingly being established in modern medical science. The shrub Ipomoea carnea has been used traditionally for thousands of years. However, there are few scientific studies on this medicinal plant, and most of the information are scattered. In this review, we have summarized the existing knowledge and recent progress on the medicinal importance of I. carnea. Different extracts of I. carnea plant possess anti-bacterial, anti-fungal, anti-oxidant, anti-cancer, anti-convulsant, immunomodulatory, anti-diabetic, hepatoprotective, anti-inflammatory, anxiolytic, sedative and wound healing activities. However, some toxicological effects have been also reported. Some of the major phytochemicals associated with the bioactivity of I. carnea have been characterized, which have been discussed in this study too. This review article might be beneficial for phytotherapy research, as I. carnea can be a good source for drug development.

  5. The effect of coloured light on Ipomoea purpurea growth

    Science.gov (United States)

    Surducan, Vasile; Lung, Ildiko; Surducan, Emanoil

    2009-08-01

    Ipomoea purpurea is a climbing ornamental plant native to Mexico. The paper is describing the experimental setup and results for indoor growing plants exposed to white LED light (inside a reference chamber) and four different wavelength LED lights (inside a measure chamber). Four growing experiments of 12-15 days, took place in identical environmental conditions (identical temperature and relative humidity inside the reference and measure chambers, similar lighting conditions and soil moisture). At the end of the experiments, the plant chlorophyll and xanthophylls content have been measured and the plant aspect (vegetal mass, leaves colour and robustness) has been observed. The smallest content in chlorophyll (a and b) was developed by the plants growth in blue light (480 nm), however those plants where 10% taller than plants growth in white light, but less robust. The higher content in carotenoids and xanthophylls was observed in plants which growth in white and red light.

  6. ASPECTOS SOBRE A BIOLOGIA DE Euchroma gigantea (L. 1758 (COLEOPTERA -BUPRESTIDAE EM Paquira aquatica AUBLET (BOMBACACEAE ASPECTS OF Euchroma gigantea (L., 1758 (COLEOPTERA-BUPRESTIDAE ON Paquira aquatica AUBLET (BOMBACACEAE

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    Antônio Henrique Garcia

    2007-09-01

    Full Text Available

    Estudou-se a biologia de E. gigantea em P. aquatica sem controle de T°C, UR e fotoperíodo. As fêmeas realizam a postura entre os meses de dezembro e março. Cada fêmea coloca massas de ovos de até 10 ovos/massa, num média de 4 massas de ovos/planta por entre as rachaduras da casca na região do colo. O período médio de incubação observado foi de 19 dias. A média de posturas por fêmeas foi de 6,2, a média de ovos/postura de 40 e o número médio de ovos/fêmea de 248, com viabilidade de 72%. As larvas constroem uma galeria, inicialmente na região subcortical e, posteriormente, no sentido do sistema radicular da planta onde completam o ciclo biológico. O período larval médio observado foi de 240 dias e as larvas medem de 8 a 12 cm de comprimento no último ínstar. A viabilidade pré-pupal foi de 63% e o período médio de 13 dias. O período pupal médio foi de 30 dias e a viabilidade, de 69%. O ciclo biológico médio foi de 302 dias. Após a emergência o adulto constrói um galeria de forma ovalada no sentido vertical em direção à superfície do solo. Como as larvas de E. gigantea destroem o sistema radicular das mungubeiras, as árvores ficam sem nenhum sistema de sustentação caindo facilmente pela ação dos ventos.

    PALAVRAS-CHAVE: Euchroma gigantea; Paquira aquatica; biologia; ornamental.

    The biology of E. gigantea on P. aquatica was studied without T°C, UR and fotoperiod. Females performed the laying of eggs from December through March. Each female lay egg masses in the plant peel crevices, averaging 4 egg masses/plant with up to 10 eggs/mass. The observed incubation period averaged 19 days. The average laying of eggs per female was 6.2, averaging 40 eggs/laying and

  7. Ipomoea batatas and Agarics blazei ameliorate diabetic disorders with therapeutic antioxidant potential in streptozotocin-induced diabetic rats.

    Science.gov (United States)

    Niwa, Atsuko; Tajiri, Takashi; Higashino, Hideaki

    2011-05-01

    Ipomoea batatas, Agaricus blazei and Smallanthus sonchifolius are known to favorably influence diabetes mellitus. To clarify their antidiabetic efficacy and hypoglycemic mechanisms, we treated streptozotocin-induced diabetic rats with daily oral feeding of powdered Ipomoea batatas (5 g kg(-1) d(-1)), Agaricus blazei (1 g kg(-1) d(-1)) or Smallanthus sonchifolius (4 g kg(-1) d(-1)) for 2 months. Treatments with Ipomoea batatas or Agaricus blazei, but not Smallanthus sonchifolius, significantly suppressed the increases of fasting plasma glucose and hemoglobin A1c levels, and restored body weight loss during diabetes. Serum insulin levels after oral glucose administration tests increased along the treatments of Ipomoea batatas or Agaricus blazei. Moreover, Ipomoea batatas and Agaricus blazei reduced superoxide production from leukocytes and vascular homogenates, serum 8-oxo-2'-deoxyguanosine, and vascular nitrotyrosine formation of diabetic rats to comparable levels of normal control animals. Stress- and inflammation-related p38 mitogen-activated protein kinase activity and tumor necrosis factor-α production of diabetic rats were significantly depressed by Ipomoea batatas administration. Histological examination also exhibited improvement of pancreatic β-cells mass after treatments with Ipomoea batatas or Agaricus blazei. These results suggest that hypoglycemic effects of Ipomoea batatas or Agaricus blazei result from their suppression of oxidative stress and proinflammatory cytokine production followed by improvement of pancreatic β-cells mass.

  8. Metal Uptake Rates by Veronica anagallis aquatica at Pinal Creek near Globe, Arizona.

    Science.gov (United States)

    Robbins, E. A.; Conklin, M.; Harvey, J.; Corley, T.

    2002-12-01

    Pinal Creek, Globe, Arizona had elevated levels of dissolved manganese [Mn(II)] in the stream that has since been intercepted, due to recharge of contaminated ground water from numerous Cu mining operations. The focus of this study is to determine the distribution of metals on water speedwell and the rate of metal removal (Mn, Co, Ni, and Zn) from surface water by the aquatic plant `water speedwell', Veronica anagallis aquatica. Water speedwell grows in the central stream channel at Pinal Creek and is suspected to bio accumulate metals as well as provide surfaces for metal precipitation. Water speedwell was collected approximately monthly from Pinal Creek for one year. Roots and shoots were separated to determine external metal concentrations, and the plants were extracted using 0.03M EDTA and 0.03M ascorbic acid. Water speedwell accumulates substantial manganese in its root and shoot tissues with median values of 22,000 mg/kg dry root and 1,700 mg/kg dry shoot, respectively. The roots of water speedwell are capable of accumulating metals at concentrations well above sediment (7,000 mg/kg dry sediment) and surface water, with (1 mg/L) the majority of metals associated with the external plant surface. To determine metal uptake rates, a known mass of plants was placed in a container with artificial surface water that had been spiked with known metal concentrations and the change in metal concentration solution was monitored over time. Manganese, nickel, and cobalt followed similar trends of an initial rapid decrease in metal from solution followed by a slower uptake rate. As much as 50% of the manganese and nickel were removed from solution over a five-day period. Manganese was removed from surface water at an average rate of -0.0016 +/- 0.0007 (Log(mgMn)/hour). Removal of zinc from surface water was correlated with pH of the solution, which ranged from 6.0 to 7.5. The results indicate that water speedwell is effective in the removal of metals from surface water.

  9. PROPRIEDADES FUNCIONAIS DAS PROTEÍNAS DE AMÊNDOAS DA MUNGUBA (Pachira aquatica Aubl.

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    BERNADETE DE LOURDES DE ARAÚJO SILVA

    2015-03-01

    Full Text Available RESUMO A semente da munguba (Pachira aquatica Aubl. contém amêndoas que exibem um conteúdo excelente de óleo e um percentualsignificativo em proteínas. Propositou-se determinar algumas propriedades funcionais das proteínas de amêndoas damunguba com o objetivo de instituir sua utilização na indústria de alimentos. O teor lipídico foi de 46,62%, o proteico de 13,75% e na forma de torta apresentou um índice de 28,27% de proteínas. Obtiveram-se doisisolados proteicos, o IP 2,0 e o IP 10,0, decorrentes de duas condições de pH (2,0 e 10,0. Na obtenção dos isolados proteicos, os índices em proteínas extraídas foram de 38,52% para o IP 2,0 e 82,06% para o IP 10,0. Os índices de proteínas recuperadas através da precipitação isoelétrica foram de 23,35% para o IP 2,0 e de 70,94%para o IP 10,0, em pH 5,0. As propriedades funcionais exibiram solubilidade mínima em pH 5,0, no pontoisoelétrico (pI, sendo mais elevada em pH ácido e alcalino do pI. As melhores capacidades de absorçãode água e de óleo exibidas foram para o IP 10,0. As propriedades emulsificantes foram dependentes do pH para os dois isolados, e o IP 10,0 indicou melhores resultados. As propriedades funcionais estudadas permitem o emprego dos isolados proteicos em produtos alimentícios que requerem alta solubilidade, tais como os produtos de panificação, massas em geral, sopas desidratadas e molhos, produtos que exigem desempenho na absorção do óleo, como as carnes simuladas, e em produtos que requerem poderes emulsificantes.

  10. 不同种植密度对水生蕹菜生长指标的影响%Effects of Different Planting Densities on Growth Indexes of Aquatic Ipomea aquatica

    Institute of Scientific and Technical Information of China (English)

    赵犇; 宛汉斌; 王立华; 王锡明; 钱娣; 闫海霞; 曹布霆

    2014-01-01

    In order to estimate the appropriate planting density of aquatic Ipomea aquatica, we set three row spacings 20 cm (P20), 30 m (P30) and 40 cm (P40) with the line spacing of 30 cm, and then studied the effects of different planting densities on growth indexes and biomass accumulation of I. aquatica. The results showed that, the main-stem height, leaf number and main-stem width of I. aquatica were characterized by P20P20>P40,因此在行距为30 cm时,最适宜水生蕹菜的生长株距为30 cm。

  11. Possible glyphosate tolerance mechanism in pitted morningglory (Ipomoea lacunosa L.).

    Science.gov (United States)

    Ribeiro, Daniela N; Nandula, Vijay K; Dayan, Franck E; Rimando, Agnes M; Duke, Stephen O; Reddy, Krishna N; Shaw, David R

    2015-02-18

    Natural tolerance of Ipomoea lacunosa to glyphosate has made it problematic in the southeastern U.S. since the adoption of glyphosate-resistant crops. Experiments were conducted to determine (i) the variability in tolerance to glyphosate among accessions, (ii) if there is any correlation between metabolism of glyphosate to aminomethylphosponic acid (AMPA) or sarcosine and the level of tolerance, and (iii) the involvement of differential translocation in tolerance to glyphosate. Fourteen I. lacunosa accessions had GR50 values ranging from 58 to 151 grams of acid equivalent per hectare (ae/ha) glyphosate, a 2.6-fold variability in tolerance to glyphosate. There was no evidence of the most tolerant (MT) accession metabolizing glyphosate to AMPA more rapidly than the least tolerant (LT) accession. Metabolism to sarcosine was not found. (14)C-glyphosate absorption was similar in the two accessions. LT accession translocated more (14)C-glyphosate than MT accession at 24 and 48 h after treatment. Differential translocation partly explains glyphosate tolerance in MT accession.

  12. Is Allelopathic Activity of Ipomoea murucoides Induced by Xylophage Damage?

    Science.gov (United States)

    Flores-Palacios, Alejandro; Corona-López, Angélica María; Rios, María Yolanda; Aguilar-Guadarrama, Berenice; Toledo-Hernández, Víctor Hugo; Rodríguez-López, Verónica; Valencia-Díaz, Susana

    2015-01-01

    Herbivory activates the synthesis of allelochemicals that can mediate plant-plant interactions. There is an inverse relationship between the activity of xylophages and the abundance of epiphytes on Ipomoea murucoides. Xylophagy may modify the branch chemical constitution, which also affects the liberation of allelochemicals with defense and allelopathic properties. We evaluated the bark chemical content and the effect of extracts from branches subjected to treatments of exclusion, mechanical damage and the presence/absence of epiphytes, on the seed germination of the epiphyte Tillandsia recurvata. Principal component analysis showed that branches without any treatment separate from branches subjected to treatments; damaged and excluded branches had similar chemical content but we found no evidence to relate intentional damage with allelopathy; however 1-hexadecanol, a defense volatile compound correlated positively with principal component (PC) 1. The chemical constitution of branches subject to exclusion plus damage or plus epiphytes was similar among them. PC2 indicated that palmitic acid (allelopathic compound) and squalene, a triterpene that attracts herbivore enemies, correlated positively with the inhibition of seed germination of T. recurvata. Inhibition of seed germination of T. recurvata was mainly correlated with the increment of palmitic acid and this compound reached higher concentrations in excluded branches treatments. Then, it is likely that the allelopathic response of I. murucoides would increase to the damage (shade, load) that may be caused by a high load of epiphytes than to damage caused by the xylophages. PMID:26625350

  13. Is Allelopathic Activity of Ipomoea murucoides Induced by Xylophage Damage?

    Directory of Open Access Journals (Sweden)

    Alejandro Flores-Palacios

    Full Text Available Herbivory activates the synthesis of allelochemicals that can mediate plant-plant interactions. There is an inverse relationship between the activity of xylophages and the abundance of epiphytes on Ipomoea murucoides. Xylophagy may modify the branch chemical constitution, which also affects the liberation of allelochemicals with defense and allelopathic properties. We evaluated the bark chemical content and the effect of extracts from branches subjected to treatments of exclusion, mechanical damage and the presence/absence of epiphytes, on the seed germination of the epiphyte Tillandsia recurvata. Principal component analysis showed that branches without any treatment separate from branches subjected to treatments; damaged and excluded branches had similar chemical content but we found no evidence to relate intentional damage with allelopathy; however 1-hexadecanol, a defense volatile compound correlated positively with principal component (PC 1. The chemical constitution of branches subject to exclusion plus damage or plus epiphytes was similar among them. PC2 indicated that palmitic acid (allelopathic compound and squalene, a triterpene that attracts herbivore enemies, correlated positively with the inhibition of seed germination of T. recurvata. Inhibition of seed germination of T. recurvata was mainly correlated with the increment of palmitic acid and this compound reached higher concentrations in excluded branches treatments. Then, it is likely that the allelopathic response of I. murucoides would increase to the damage (shade, load that may be caused by a high load of epiphytes than to damage caused by the xylophages.

  14. Secretory structures of Ipomoea asarifolia: anatomy and histochemistry

    Directory of Open Access Journals (Sweden)

    Fabiano M. Martins

    2012-02-01

    Full Text Available Ipomoea asarifolia (Desr. Roem. & Schult., Convolvulaceae, is a weed that infests agricultural areas and is toxic to cattle. In spite of its toxicity, the leaves of this plant are used in traditional remedies in the state of Bahia, Brazil. The present work describes the leaf anatomy of I. asarifolia and characterizes the exudates of its secretory structures. The leaves have a unistratified epidermis composed of ordinary cells with straight to slightly sinuous anticlinal walls and thin cuticles. Paracytic stomata are found on both surfaces of the leaves at the same level as the ordinary epidermal cells. Trichomes producing polysaccharide secretions occur on the petiole and leaf blade and are considered colleters. The mesophyll is dorsiventral and the vascular bundle of the central vein is bicollateral. Two opposed nectaries occur on the petiole near the leaf blade. Each nectary is composed of a small canal with internal ramifications and numerous secretory trichomes. The laticiferous glands are articulated, not anastomosed, and are composed of large diameter cells with thin cell walls. The secretions of the laticiferous glands are lipidic.

  15. MORPHOPHYSIOLOGICAL CHANGES OF IPOMOEA BATATAS (L. LAM. DURING DROUGHT STRESS

    Directory of Open Access Journals (Sweden)

    La Rosa, R.

    2008-01-01

    Full Text Available One of the greatest problems in the world is desertification so we must to know physiological responses and morphological adaptations from species with tolerance to drought stress. Sweet potato (Ipomoea batatas Lam. is one of the most important crops of the world, especially because of its great tolerance to drought stress. Hence, we decided to know what morphophysiological and metabolic responses has I. batatas under drought stress. So, we got cuttings of variety INA-100, from INIA Huaral, and were planted in 22 recipients of 4 L with substrate consisting in a mixing of sand and humus (3:1. Half of them were irrigated monthly and other half only receives water to establish plants and no more after that. We measured transpiration, histological changes in leaves and metabolic changes at protein and starch level. We confirmed transpiration is not affected for air humidity or substrate humidity. But, the effect of drought can be better observed in foliage growth, therefore in histological changes in leaves. No significance in concentration of proteins but significance in carbohydrates concentration shows an osmotic regulation in order to maintain stomata open and also an increase in photosynthetic activity in stressed plants.

  16. Toxic effects of prenatal Ipomoea carnea administration to rats.

    Science.gov (United States)

    Hueza, I M; Dagli, M L Z; Górniak, S L; Paulino, C A

    2003-12-01

    Chronic exposure of livestock to Ipomoea carnea, a toxic plant, promotes toxicosis characterized by lysosomal vacuolization of different organs, and is clinically manifested by CNS signs, abnormal endocrine and gastrointestinal functions, alteration of the immune system, and abnormal embryogenesis. The present study evaluated the effects of different doses of the plant extract on pregnant rats and their offspring after oral administration to the dams from day 6 to day 20 of gestation. Histopathology of thyroid, pancreas, liver and kidneys of dams on gestational day 21 showed characteristic vacuolization promoted by I. carnea toxicosis in these organs; the same was observed in the organs of 7-d-old pups. On the other hand, no alteration was found in these same organs of dams the 7th d after parturition. Although the lesions were reversed in the dams, the same did not occur in their pups. I. carnea administration also promoted decreased body weight, thymus atrophy and spleen enlargement in pups. The toxic principle of I. carnea (swainsonine) seems to pass through the placenta.

  17. Is Allelopathic Activity of Ipomoea murucoides Induced by Xylophage Damage?

    Science.gov (United States)

    Flores-Palacios, Alejandro; Corona-López, Angélica María; Rios, María Yolanda; Aguilar-Guadarrama, Berenice; Toledo-Hernández, Víctor Hugo; Rodríguez-López, Verónica; Valencia-Díaz, Susana

    2015-01-01

    Herbivory activates the synthesis of allelochemicals that can mediate plant-plant interactions. There is an inverse relationship between the activity of xylophages and the abundance of epiphytes on Ipomoea murucoides. Xylophagy may modify the branch chemical constitution, which also affects the liberation of allelochemicals with defense and allelopathic properties. We evaluated the bark chemical content and the effect of extracts from branches subjected to treatments of exclusion, mechanical damage and the presence/absence of epiphytes, on the seed germination of the epiphyte Tillandsia recurvata. Principal component analysis showed that branches without any treatment separate from branches subjected to treatments; damaged and excluded branches had similar chemical content but we found no evidence to relate intentional damage with allelopathy; however 1-hexadecanol, a defense volatile compound correlated positively with principal component (PC) 1. The chemical constitution of branches subject to exclusion plus damage or plus epiphytes was similar among them. PC2 indicated that palmitic acid (allelopathic compound) and squalene, a triterpene that attracts herbivore enemies, correlated positively with the inhibition of seed germination of T. recurvata. Inhibition of seed germination of T. recurvata was mainly correlated with the increment of palmitic acid and this compound reached higher concentrations in excluded branches treatments. Then, it is likely that the allelopathic response of I. murucoides would increase to the damage (shade, load) that may be caused by a high load of epiphytes than to damage caused by the xylophages.

  18. Pollination biology of an invasive weed Ipomoea cairica (Convolvulaceae) in Guangdong Province, China

    OpenAIRE

    Xiaocheng Jia; Xinliang Li; Yang Dan; Guohui Lu; Yingqiang Wang

    2007-01-01

    During May to July, 2006 and April to May, 2007, we studied pollination biology in Ipomoea cairica, an invasive weed in Guangdong Province, China. Ipomoea cairica is a perennial creeping or climbing herbaceous vine, blooming all year round in Guangdong. The flowers gathered in cymes, with a purple or bluish purple bell-formed corolla. The petals unfolded at about 4:30–5:20 and closed at 17:40, lasting for about 12 hours. The proximity of the stigma and anthers during flowering period facilita...

  19. Gamma irradiation effect on the chemical composition and the antioxidant activity of Ipomoea batatas L

    Energy Technology Data Exchange (ETDEWEB)

    Tahir, D., E-mail: dtahir@fmipa.unhas.ac.id; Halide, H., E-mail: dtahir@fmipa.unhas.ac.id; Kurniawan, D. [Department of Physics, Hasanuddin University, Makassar 90245 (Indonesia); Wahab, A. W. [Department of Chemistry, Hasanuddin University, Makassar 90245 (Indonesia)

    2014-09-25

    The chemical composition and antioxidant activity of Ipomoea batatas L. (sweet potato) were studied by x-ray fluorescence (XRF) spectroscopy, Fourier transform infrared spectroscopy, and by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity. The irradiation treatment was performed by using Cs-137 as a gamma sources in experimental equipment. Treatment by irradiation emerges as a possible conservation technique that has been tested successfully in several food products. The amount of chemical composition was changed and resulting new chemical for absorbed dose 40 mSv. Interestingly, it was found that gamma irradiation significantly increased the antioxidant activity, as measured by DPPH radical scavenging capacity. The antioxidant activity of Ipomoea batatas L. extract was dramatically increased in the non-irradiated sample to the sample irradiated at 40 mSv. These results indicate that gamma irradiation of Ipomoea batatas L. extract can enhance its antioxidant activity through the formation of a new chemical compound. Based on these results, increased antioxidant activity of Ipomoea batatas L. extracts by gamma rays can be applied to various industries, especially cosmetics, foodstuffs, and pharmaceuticals.

  20. Nuclear fragmentation and DNA degradation during programmed cell death in petals of morning glory (Ipomoea nil)

    NARCIS (Netherlands)

    Yamada, T.; Takatsu, Y.; Kasumi, K.; Ichimura, K.; Doorn, van W.G.

    2006-01-01

    We studied DNA degradation and nuclear fragmentation during programmed cell death (PCD) in petals of Ipomoea nil (L.) Roth flowers. The DNA degradation, as observed on agarose gels, showed a large increase. Using DAPI, which stains DNA, and flow cytometry for DAPI fluorescence, we found that the num

  1. Gamma irradiation effect on the chemical composition and the antioxidant activity of Ipomoea batatas L.

    Science.gov (United States)

    Tahir, D.; Halide, H.; Wahab, A. W.; Kurniawan, D.

    2014-09-01

    The chemical composition and antioxidant activity of Ipomoea batatas L. (sweet potato) were studied by x-ray fluorescence (XRF) spectroscopy, Fourier transform infrared spectroscopy, and by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity. The irradiation treatment was performed by using Cs-137 as a gamma sources in experimental equipment. Treatment by irradiation emerges as a possible conservation technique that has been tested successfully in several food products. The amount of chemical composition was changed and resulting new chemical for absorbed dose 40 mSv. Interestingly, it was found that gamma irradiation significantly increased the antioxidant activity, as measured by DPPH radical scavenging capacity. The antioxidant activity of Ipomoea batatas L. extract was dramatically increased in the non-irradiated sample to the sample irradiated at 40 mSv. These results indicate that gamma irradiation of Ipomoea batatas L. extract can enhance its antioxidant activity through the formation of a new chemical compound. Based on these results, increased antioxidant activity of Ipomoea batatas L. extracts by gamma rays can be applied to various industries, especially cosmetics, foodstuffs, and pharmaceuticals.

  2. Nuclear fragmentation and DNA degradation during programmed cell death in petals of morning glory (Ipomoea nil)

    NARCIS (Netherlands)

    Yamada, T.; Takatsu, Y.; Kasumi, K.; Ichimura, K.; Doorn, van W.G.

    2006-01-01

    We studied DNA degradation and nuclear fragmentation during programmed cell death (PCD) in petals of Ipomoea nil (L.) Roth flowers. The DNA degradation, as observed on agarose gels, showed a large increase. Using DAPI, which stains DNA, and flow cytometry for DAPI fluorescence, we found that the

  3. Chemical optimization of protein extraction from sweet potato (Ipomoea batatas) peel

    Science.gov (United States)

    Proteins isolated from sweet potatoes (Ipomoea batatas) have been shown to possess antidiabetic, antioxidant, and antiproliferative properties. The objective of this study was to chemically optimize a process for extracting proteins from sweet potato peel. The extraction procedure involved mixing pe...

  4. Improvement of cryopreservation technique for long-term storage of shoot tips of Ipomoea batatas

    Science.gov (United States)

    Roots of sweet potato (Ipomoea batatis) are an important food crop in sub-tropical and tropical regions. Being a vegetatively propagated crop, its genetic resources are predominantly preserved as field plantings and/or as tissue cultures. Cryopreservation is the most economic and reliable preservati...

  5. Conditioned food aversion to control outbreaks of intoxication by Ipomoea carnea and Turbina cordata in goats

    Science.gov (United States)

    Conditioned food aversion is used to train livestock to avoid the ingestion of toxic plants. This technique was used to control Turbina cordata poisoning in goats in one farm, and to control Ipomoea carnea subsp. istulosa poisoning in another farm. The goats were penned at night and the next mornin...

  6. Phyto-Toxicity and Uptake of Cadmium by Brassica chinensis and Ipomoea aquatica in Soils%土壤Cd污染对青菜和蕹菜生长及Cd含量的影响

    Institute of Scientific and Technical Information of China (English)

    孙兆海; 郑春荣; 周东美; 杨林章; 陈怀满

    2005-01-01

    通过温室盆栽试验,研究了草甸棕壤、红壤和灰色石灰土的人为Cd污染对青菜和蕹菜生物量和Cd含量的影响.结果表明,红壤加入Cd浓度为0.5、1.0、3.0、5.0 mg·kg-1时,青菜地上部分的生物量与对照相比分别降低了13%、15%、25%和91%;蕹菜地上部分的生物量在添加Cd浓度为0.5、1.0、3.0 mg·kg-1时与对照相比分别增加了21%、64%和81%,而添加浓度为5.0和10.0 mg·kg-1时则分别降低了63%和86%;生长于草甸棕壤和灰色石灰土中的蕹菜地上部分的生物量随土壤Cd浓度的增加无显著差异.在土壤添加Cd浓度相同的条件下,青菜和蕹菜地上部分的Cd含量在3种土壤中的顺序为:灰色石灰土<草甸棕壤<红壤.在试验条件下,以国家食品卫生标准为依据、以青菜为指示植物计算得到的3种土壤Cd的表观临界含量分别为:以总Cd计算:草甸棕壤为0.33 mg·kg-1,红壤为0.066 mg·kg-1,灰色石灰土为0.70 mg·kg-1;以DTPA提取态Cd计算:草甸棕壤为0.13 mg·kg-1,红壤为0.012 mg·kg-1,灰色石灰土为0.33 mg·kg-1.

  7. Relaxed Constraint and Evolutionary Rate Variation between Basic Helix-Loop-Helix Floral Anthocyanin Regulators in Ipomoea

    National Research Council Canada - National Science Library

    Streisfeld, Matthew A; Rausher, Mark D

    2007-01-01

    .... We cloned the full-length coding region from 2 basic helix-loop-helix transcription factors from several species of Ipomoea with diverse flower colors and determined the selective forces operating on them...

  8. Development of successive cambia, cambial activity, and their relationship to physiological traits in Ipomoea arborescens (Convolvulaceae) seedlings

    National Research Council Canada - National Science Library

    Teresa Terrazas; Silvia Aguilar-Rodríguez; Clara Tinoco Ojanguren

    2011-01-01

    .... We studied the anatomy of Ipomoea arborescens seedlings to define when cambium is differentiated, to determine how many cambia differentiate in one year of growth, and to relate the development...

  9. Rhizobium ipomoeae sp. nov., isolated from a water convolvulus field.

    Science.gov (United States)

    Sheu, Shih-Yi; Chen, Zih-Han; Young, Chiu-Chung; Chen, Wen-Ming

    2016-04-01

    A bacterial strain, designated shin9-1T, was isolated from a water sample taken from a water convolvulus field in Taiwan and characterized using a polyphasic taxonomical approach. Cells of strain shin9-1T were aerobic, Gram-stain-negative, rod-shaped and surrounded by a thick capsule and formed cream-coloured colonies. Growth occurred at 10-45 °C (optimum, 30 °C), with 0-3.0% NaCl (optimum, 0.5%) and at pH 7.0-9.0 (optimum, pH 7.0). Strain shin9-1T did not form nodules on a legume plant, Macroptilium atropurpureum, and the nodulation genes nodA, nodC and the nitrogenase reductase gene nifH were not detected by PCR. Phylogenetic analyses based on 16S rRNA and three housekeeping gene sequences (recA, atpD and rpoB) showed that strain shin9-1T belonged to the genus Rhizobium. Strain shin9-1T had the highest level of 16S rRNA gene sequence similarity with respect to Rhizobium daejeonense L61T (97.6 %). The major fatty acid of strain shin9-1T was C18:1ω7c. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, phosphatidylmonomethylethanolamine and several uncharacterized lipids. The DNA G+C content was 58.3 mol%. The DNA-DNA relatedness of strain shin9-1T with respect to recognized species of the genus Rhizobium was less than 70%. Phenotypic characteristics of the novel strain also differed from those of the most closely related species of the genus Rhizobium. On the basis of the phylogenetic inference and phenotypic data, strain shin9-1T should be classified as a representative of a novel species, for which the name Rhizobium ipomoeae sp. nov. is proposed. The type strain is shin9-1T (=LMG 27163T=KCTC 32148T).

  10. Description of Pelomonas aquatica sp. nov. and Pelomonas puraquae sp. nov., isolated from industrial and haemodialysis water.

    Science.gov (United States)

    Gomila, Margarita; Bowien, Botho; Falsen, Enevold; Moore, Edward R B; Lalucat, Jorge

    2007-11-01

    Three Gram-negative, rod-shaped, non-spore-forming bacteria (strains CCUG 52769T, CCUG 52770 and CCUG 52771) isolated from haemodialysis water were characterized taxonomically, together with five strains isolated from industrial waters (CCUG 52428, CCUG 52507, CCUG 52575T, CCUG 52590 and CCUG 52631). Phylogenetic analysis based on 16S rRNA gene sequences indicated that these isolates belonged to the class Betaproteobacteria and were related to the genus Pelomonas, with 16S rRNA gene sequence similarities higher than 99% with the only species of the genus, Pelomonas saccharophila and to Pseudomonas sp. DSM 2583. The type strains of Mitsuaria chitosanitabida and Roseateles depolymerans were their closest neighbours (97.9 and 97.3% 16S rRNA gene sequence similarity, respectively). Phylogenetic analysis was also performed for the internally transcribed spacer region and for three genes [hoxG (hydrogenase), cbbL/cbbM (Rubisco) and nifH (nitrogenase)] relevant for the metabolism of the genus Pelomonas. DNA-DNA hybridization, major fatty acid composition and phenotypical analyses were carried out, which included the type strain of Pelomonas saccharophila obtained from different culture collections (ATCC 15946T, CCUG 32988T, DSM 654T, IAM 14368T and LMG 2256T), as well as M. chitosanitabida IAM 14711T and R. depolymerans CCUG 52219T. Results of DNA-DNA hybridization, physiological and biochemical tests supported the conclusion that strains CCUG 52769, CCUG 52770 and CCUG 52771 represent a homogeneous phylogenetic and genomic group, including strain DSM 2583, clearly differentiated from the industrial water isolates and from the Pelomonas saccharophila type strain. On the basis of phenotypic and genotypic characteristics, these strains belong to two novel species within the genus Pelomonas, for which the names Pelomonas puraquae sp. nov. and Pelomonas aquatica sp. nov. are proposed. The type strains of Pelomonas puraquae sp. nov. and Pelomonas aquatica sp. nov. are CCUG

  11. Identificação de princípios ativos presentes na Ipomoea carnea brasileira Identification of Brazilian Ipomoea carnea toxic compounds

    Directory of Open Access Journals (Sweden)

    Aline Schwarz

    2004-06-01

    Full Text Available Dentre as espécies pertencentes à família das Convolvulaceae destacam-se as Ipomoeas, amplamente distribuídas por todo o mundo, bastante conhecidas e cultivadas devido ao aspecto ornamental que suas flores campanuladas e de cores vibrantes oferecem. É sabido porém que espécies de Ipomoeas são tóxicas. A Ipomoea carnea, espécie de nosso estudo, provoca emagrecimento, apatia, incoordenção motora, fraqueza progressiva e até mesmo a morte em animais de produção, se ingerida por período prolongado. Os alcalóides suainsonina e calisteginas presentes nesta planta são certamente responsáveis por tais efeitos tóxicos, já que inibem a ação das manosidases e glicosidases, enzimas fundamentais para um adequado metabolismo de carboidratos pelo organismo. O presente trabalho teve como objetivo verificar e caracterizar os constituintes químicos da I. carneabrasileira. Assim, empregando-se as cromatografias de camada delgada e líquida acoplada a detector de massas, além da ressonância nuclear de prótons e carbono, foram detectados no extrato aquoso obtido das folhas da planta, 0,09% de suainsonina, 0,11% de calistegina B2, 0,14% de calistegina B1, 0,06% de calistegina C1 e o aminoácido não protéico N-metil-trans-4-hidroxi-L-prolina.In the Convolvulaceae family, the Ipomoeas species are cultivated and found in all regions of the world because of their ornamental bright coloured flowers. It is well known that some Ipomoeas species are toxic. Ipomoea carnea, species of this study, causes depression, general weakness, loss of body weight, stagering gait and death of animals after prolonged periods of plant intake. These toxic effects are attributed to the alkaloids swainsonine and calystegines present in the plant, wich promotes inhibition of galactosidases and manosidases, important enzymes for an adequate metabolism of carbohydrates in the organism. The objective of the present study was to detect and characterize the chemical

  12. Floral biology and breeding system of three Ipomoea weeds Biologia floral e sistema reprodutivo de três espécies daninhas de Ipomoea

    Directory of Open Access Journals (Sweden)

    R.C.S. Maimoni-Rodella

    2007-03-01

    Full Text Available The floral biology of three weeds, Ipomoea cairica, I. grandifolia and I. nil (Convolvulaceae, was studied in Botucatu and Jaboticabal, São Paulo, in southeastern Brazil. The three species are melittophilous, with a varied set of floral visitors, but with some overlapping. Cluster analysis using Jacquard similarity index indicated a greater similarity among different plant species in the same locality than among the populations at different places, in relation to floral visitor sets. The promiscuous and opportunistic features of the flowers were shown, with such type of adaptation to pollination being advantageous to weeds since pollinator availability is unpredictable at ruderal environments.A biologia floral de Ipomoea cairica, I. grandifolia e I. nil - plantas daninhas da família Convolvulaceae - foi estudada em Botucatu e Jaboticabal, Estado de São Paulo, Brasil. As três espécies são melitófilas, apresentando conjuntos de visitantes florais bastante diversificados, embora haja alguma sobreposição entre eles. Com relação aos visitantes florais, a análise de agrupamento, empregando-se o índice de similaridade de Jaccard, indicou maior similaridade entre diferentes espécies de Ipomoea ocorrentes no mesmo local do que entre populações da mesma espécie em diferentes localidades. O caráter promíscuo e oportunista da adaptação à polinização, presente nessas espécies, foi demonstrado, sendo essa adaptação vantajosa para plantas daninhas, uma vez que em ambientes ruderais a disponibilidade de polinizadores é imprevisível.

  13. Changes in swainsonine, calystegine, and nitrogen concentrations on an annual basis in Ipomoea carnea.

    Science.gov (United States)

    Cook, Daniel; Oliveira, Carlos A; Gardner, Dale R; Pfister, James A; Riet-Correa, Gabriela; Riet-Correa, Franklin

    2015-03-01

    Ipomoea carnea, a swainsonine containing plant, is known to cause a neurologic disease in grazing livestock in Brazil and other parts of the world. To better understand the relative toxicity and nutritional content of I. carnea we investigated swainsonine, calystegine, and crude protein concentrations in leaves of I. carnea on a monthly basis for one year in northern and northeastern Brazil. Swainsonine concentrations were detected at concentrations that could potentially poison an animal throughout the year although there was some variation between months. At one location swainsonine concentrations were generally the highest during the rainy season or the months immediately following the rainy season. Total calystegine concentrations were similar to those reported previously while crude protein concentrations were similar to those found in other Ipomoea species and are such that they may explain why I. carnea becomes desirable to grazing livestock as forage becomes limited during the dry season.

  14. Enhanced phytoremediation of cadmium polluted water through two aquatic plants Veronica anagallis-aquatica and Epilobium laxum.

    Science.gov (United States)

    Ahmad, Ayaz; Hadi, Fazal; Ali, Nasir; Jan, Amin Ullah

    2016-09-01

    Toxic metal-contaminated water is a major threat to sustainable agriculture and environment. Plants have the natural ability to absorb and concentrate essential elements in its tissues from water solution, and this ability of plants can be exploited to remove heavy/toxic metals from the contaminated water. For this purpose, two plants Veronica anagallis-aquatica and Epilobium laxum were hydroponically studied. The effect of different fertilizers (NPK) and plant growth regulators (GA3 and IAA) were evaluated on growth, biomass, free proline, phenolics, and chlorophyll contents, and their role in Cd phytoaccumulation was investigated. Results showed that in both plants, fertilizer addition to media (treatment T4) produced the highest significant increase in growth, biomass (fresh and dry), cadmium concentration, proline, phenolics, and chlorophyll concentrations. The significant effect of GA3 in combination with NPK foliar spray (treatment T12) was observed on most of the growth parameters, Cd concentration, and proline and phenolic contents of the plants. The free proline and total phenolics showed positive correlation with cadmium concentration within plant tissues. Proline showed significantly positive correlation with phenolic contents of root and shoot. Veronica plant demonstrated the hyperaccumulator potential for cadmium as bioconcentration factor (BCF >1) which was much higher than 1, while Epilobium plant showed non-hyperaccumulator potential. It is recommended for further study to investigate the role of Veronica plant for other metals and to study the role of phenolics and proline contents in heavy metal phytoextraction by various plant species.

  15. Dye-sensitized solar cell using natural dyes extracted from spinach and ipomoea

    Energy Technology Data Exchange (ETDEWEB)

    Chang, H., E-mail: f10381@ntut.edu.t [Department of Mechanical Engineering, National Taipei University of Technology, No. 1. Sec. 3, Chung-Hsiao E. Rd., Taipei 10608, Taiwan (China); Wu, H.M. [Department of Materials Engineering, Tatung University, No. 40, Sec. 3, Jhongshan N. Rd. Jhongshan District, Taipei City 104, Taiwan (China); Chen, T.L. [Department of Industrial Design, National Taipei University of Technology, No. 1, Sec. 3, Chung-Hsiao E. Rd., Taipei 10608, Taiwan (China); Huang, K.D. [Department of Vehicle Engineering, National Taipei University of Technology, No. 1, Sec. 3, Chung-Hsiao E. Rd., Taipei 10608, Taiwan (China); Jwo, C.S. [Department of Energy and Air-Conditioning Refrigeration Engineering, National Taipei University of Technology, No. 1, Sec. 3, Chung-Hsiao E. Rd., Taipei 10608, Taiwan (China); Lo, Y.J. [Department of Mechanical Engineering, National Taipei University of Technology, No. 1. Sec. 3, Chung-Hsiao E. Rd., Taipei 10608, Taiwan (China)

    2010-04-16

    This study used spinach extract, ipomoea leaf extract and their mixed extracts as the natural dyes for a dye-sensitized solar cell (DSSC). Spinach and ipomoea leaves were first placed separately in ethanol and the chlorophyll of these two kinds of plants was extracted to serve as the natural dyes for using in DSSCs. In addition, the self-developed nanofluid synthesis system prepared a TiO{sub 2} nanofluid with an average particle size of 50 nm. Electrophoresis deposition was performed to let the TiO{sub 2} deposit nanoparticles on the indium tin oxide (ITO) conductive glass, forming a TiO{sub 2} thin film with the thickness of 11.61 {mu}m. This TiO{sub 2} thin film underwent sintering at 450 {sup o}C to enhance the compactness of thin film. Finally, the sintered TiO{sub 2} thin film was immersed in the natural dye solutions extracted from spinach and ipomoea leaves, completing the production of the anode of DSSC. This study then further inspected the fill factor, photoelectric conversion efficiency and incident photon current efficiency of the encapsulated DSSC. According to the experimental results of current-voltage curve, the photoelectric conversion efficiency of the DSSCs prepared by natural dyes from ipomoea leaf extract is 0.318% under extraction temperature of 50 {sup o}C and pH value of extraction fluid at 1.0. This paper also investigated the influence of the temperature in the extraction process of this kind of natural dye and the influence of pH value of the dye solution on the UV-VIS patterns absorption spectra of the prepared natural dye solutions, and the influence of these two factors on the photoelectric conversion efficiency of DSSC.

  16. EVALUATION OF SUBLETHAL EFFECTS OF Ipomoea cairica LINN. EXTRACT ON LIFE HISTORY TRAITS OF DENGUE VECTORS

    OpenAIRE

    Zuharah, Wan Fatma; AHBIRAMI, Rattanam; Dieng, Hamady; Thiagaletchumi, Maniam; Nik FADZLY

    2016-01-01

    Plant derived insecticides have considerable potential for mosquito control because these products are safer than conventional insecticides. This study aimed to investigate sublethal activities of Ipomoea carica or railway creeper crude acethonilic extract against life history trait of dengue vectors, Aedes albopictus and Aedes aegypti. The late third instar larvae of Ae. albopictus and Ae. aegypti were exposed to a sublethal dose at LC50 and larvae that survived were further cultured. Overal...

  17. DNA relatedness among strains of the sweet potato pathogen Streptomyces ipomoea (Person and Martin 1940) Waksman and Henrici 1948.

    Science.gov (United States)

    Labeda, D P; Lyons, A J

    1992-02-01

    DNA relatedness among 28 putative strains of Streptomyces ipomoea from geographically diverse locations and the type strain, NRRL B-12321, was determined spectrophotometrically. The data confirm that these 28 strains are not closely related genetically to the plant-pathogenic species Streptomyces scabies (39% DNA relatedness) or Streptomyces acidiscabies (17% DNA relatedness) or any other major blue-spored Streptomyces species (less than 30% DNA relatedness). Of the 29 strains examined, 4 could be clearly distinguished from S. ipomoea on the basis of morphological criteria, i.e., they had gray rather than blue spores and produced melanin pigment, and their low DNA relatedness to authentic S. ipomoea strains confirmed their original misidentification. The remaining 25 S. ipomoea strains exhibited high DNA relatedness among themselves (76 to 100% homology), even though they had been isolated in different locations throughout the United States and Japan. The avirulent type strain, NRRL B-12321, exhibited slightly lower DNA relatedness with the virulent strains of S. ipomoea (85% average DNA relatedness) than was observed among the virulent strains (average of 96% DNA relatedness).

  18. Estudio de la variación diaria de los derivados de ergolinas en Ipomoea carnea - Study of the daily variation of the derivatives of ergolines in Ipomoea carnea

    Directory of Open Access Journals (Sweden)

    Espartaco, Sandoval

    2010-03-01

    Full Text Available ResumenLa Ipomoea carnea es una planta perteneciente al género de las Ipomoeas, capaz de provocar una intoxicación en los caprinos caracterizada por daño hepático, trastornos nerviosos y muerte. Ante la presunción, de que esta planta no es toxica durante ciertos periodos del día, se realizo un estudio que contemplo muestreos de plantas, cada dos horas, entre las 6:00 am y 6:00 pm, posteriormente se realizó la extracción de alcaloides ergoticos con solventes orgánicos y se procedió a realizar tres pruebas para la detección de ácido d-Lisérgico: prueba de fluorescencia, prueba de color oensayo cromático y Cromatografía de capa fina. Los resultadosdemostraron que las concentraciones de alcaloides presentes en plantas de Ipomoea carnea, se mantienen estables y constantes durante todo el día representando un riesgo potencial para la salud de los rebaños caprinos. SummaryIpomoea carnea is a plant pertaining the genus of the Ipomoeas, able to generate a poisoning in the goats, it is characterized by hepatic damage, nervous upheavals and death. Before the presumption, this plant is not toxic during certain periods of the day, carry out one study which was contemplated samplings of plants, every two hours, between 6:00 a.m. and 6:00 p.m., later was made the extraction of ergoticos alkaloids with organic solvent and it was come to make three tests for the d-Lysergic acid detection: fluorescence test, color test or chromatic test and Chromatography of fine layer. The results demonstrated that the concentrations of present alkaloids in plants of Ipomoea carnea, remain stable and constant during all day representing a potential risk for the health of the goat flocks.

  19. Potencial alelopático de Ipomoea fistulosa sobre a germinação de alface e tomate = Allelopathic potential of Ipomoea fistulosa on the germination of lettuce and tomato

    Directory of Open Access Journals (Sweden)

    Juliana Domingues Lima

    2008-07-01

    Full Text Available O objetivo do presente trabalho foi determinar o potencial alelopático de Ipomoea fistulosa na germinação e crescimento de plântulas. Em laboratório, sementes de alface e de tomate foram colocadas para germinar na presença de água ou extratos de folhasde Ipomoea fistulosa, nas concentrações de 5, 10 e 15% (p/v. Em casa-de-vegetação, as sementes foram colocadas para germinar em bandejas, contendo o substrato Plantmax® e o substrato Plantmax® + resíduo de folhas secas de Ipomoea fistulosa, na proporção 3% (p/p. Oextrato aquoso inibiu a germinação e elevou o tempo médio de germinação das sementes, além de reduzir o crescimento da radícula e hipocótilo em todas as concentrações utilizadas. Em casa-de-vegetação, a altura da planta, o número de folhas, o acúmulo de massa seca das plantas também foram reduzidos na presença dos resíduos de folhas no substrato. Os resultados sugerem a presença de aleloquímicos em Ipomoea fistulosa e demonstram maior sensibilidade da alface a esses compostos, quando comparada ao tomate.The objective of the present research was to determine theallelopathic potential of Ipomoea fistulosa plants on germination and growth of seedlings. In laboratory, lettuce and tomato seeds were placed to germinate in the presence of water or Ipomoea fistulosa leaf extract, in the concentrations of 5, 10 e 15% (p/v. In greenhouse, seeds were placed to germinate in trays containing Plantmax® substrate and Plantmax® substrate + Ipomoea fistulosa dry leaf residue, at 3% (w/w. The aqueous extract inhibited germination,raised the mean germination time of the seeds and reduced the radicle and hypocotyl growth in all used concentrations. In greenhouse, the plant height, leaf number, and dry mass accumulation also were reduced in the presence of the leaf residue in the substrate.The results suggest the presence of allelochemicals in Ipomoea fistulosa and demonstrate the highest sensitivity of lettuce that tomato

  20. Analyses of the complete genome and gene expression of chloroplast of sweet potato [Ipomoea batata].

    Science.gov (United States)

    Yan, Lang; Lai, Xianjun; Li, Xuedan; Wei, Changhe; Tan, Xuemei; Zhang, Yizheng

    2015-01-01

    Sweet potato [Ipomoea batatas (L.) Lam] ranks among the top seven most important food crops cultivated worldwide and is hexaploid plant (2n=6x=90) in the Convolvulaceae family with a genome size between 2,200 to 3,000 Mb. The genomic resources for this crop are deficient due to its complicated genetic structure. Here, we report the complete nucleotide sequence of the chloroplast (cp) genome of sweet potato, which is a circular molecule of 161,303 bp in the typical quadripartite structure with large (LSC) and small (SSC) single-copy regions separated by a pair of inverted repeats (IRs). The chloroplast DNA contains a total of 145 genes, including 94 protein-encoding genes of which there are 72 single-copy and 11 double-copy genes. The organization and structure of the chloroplast genome (gene content and order, IR expansion/contraction, random repeating sequences, structural rearrangement) of sweet potato were compared with those of Ipomoea (L.) species and some basal important angiosperms, respectively. Some boundary gene-flow and gene gain-and-loss events were identified at intra- and inter-species levels. In addition, by comparing with the transcriptome sequences of sweet potato, the RNA editing events and differential expressions of the chloroplast functional-genes were detected. Moreover, phylogenetic analysis was conducted based on 77 protein-coding genes from 33 taxa and the result may contribute to a better understanding of the evolution progress of the genus Ipomoea (L.), including phylogenetic relationships, intraspecific differentiation and interspecific introgression.

  1. Transcriptome Profiling of Beach Morning Glory (Ipomoea imperati under Salinity and Its Comparative Analysis with Sweetpotato.

    Directory of Open Access Journals (Sweden)

    Julio Solis

    Full Text Available The response and adaption to salt remains poorly understood for beach morning glory [Ipomoea imperati (Vahl Griseb], one of a few relatives of sweetpotato, known to thrive under salty and extreme drought conditions. In order to understand the genetic mechanisms underlying salt tolerance of a Convolvulaceae member, a genome-wide transcriptome study was carried out in beach morning glory by 454 pyrosequencing. A total of 286,584 filtered reads from both salt stressed and unstressed (control root and shoot tissues were assembled into 95,790 unigenes with an average length of 667 base pairs (bp and N50 of 706 bp. Putative differentially expressed genes (DEGs were identified as transcripts overrepresented under salt stressed tissues compared to the control, and were placed into metabolic pathways. Most of these DEGs were involved in stress response, membrane transport, signal transduction, transcription activity and other cellular and molecular processes. We further analyzed the gene expression of 14 candidate genes of interest for salt tolerance through quantitative reverse transcription PCR (qRT-PCR and confirmed their differential expression under salt stress in both beach morning glory and sweetpotato. The results comparing transcripts of I. imperati against the transcriptome of other Ipomoea species, including sweetpotato are also presented in this study. In addition, 6,233 SSR markers were identified, and an in silico analysis predicted that 434 primer pairs out of 4,897 target an identifiable homologous sequence in other Ipomoea transcriptomes, including sweetpotato. The data generated in this study will help in understanding the basics of salt tolerance of beach morning glory and the SSR resources generated will be useful for comparative genomics studies and further enhance the path to the marker-assisted breeding of sweetpotato for salt tolerance.

  2. Oviposition deterring and oviciding potentials of Ipomoea cairica L. leaf extract against dengue vectors.

    Science.gov (United States)

    Ahbirami, Rattanam; Zuharah, Wan Fatma; Yahaya, Zary Shariman; Dieng, Hamady; Thiagaletchumi, Maniam; Fadzly, Nik; Ahmad, Abu Hassan; Abu Bakar, Sazaly

    2014-09-01

    Bioprospecting of plant-based insecticides for vector control has become an area of interest within the last two decades. Due to drawbacks of chemical insecticides, phytochemicals of plant origin with mosquito control potential are being utilized as alternative sources in integrated vector control. In this regard, the present study aimed to investigate oviposition deterring and oviciding potentials of Ipomoea cairica (L.) (Family: Convolvulaceae) crude leaf extract against dengue vectors, Aedes aegypti and Aedes albopictus. Ipomoea cairica is an indigenous plant that has demonstrated marked toxicity towards larvae of Ae. aegypti and Ae. albopictus. Leaves of I. cairica were extracted using Soxhlet apparatus with acetone as solvent. Oviposition deterrent activity and ovicidal assay was carried out in oviposition site choice tests with three different concentrations (50, 100, 450 ppm). Acetone extract of I. cairica leaf strongly inhibited oviposition with 100% repellence to Ae. aegypti at lower concentration of 100 ppm, while for Ae. albopictus was at 450 ppm. The oviposition activity index (OAI) values which ranged from -0.69 to -1.00 revealed that I. cairica demonstrated deterrent effect. In ovicidal assay, similar trend was observed whereby zero hatchability was recorded for Ae. aegypti and Ae. albopictus eggs at 100 and 450 ppm, respectively. It is noteworthy that I. cairica leaf extract had significantly elicited dual properties as oviposition deterrent and oviciding agent in both Aedes species. Reduction in egg number through oviposition deterring activity, reduction in hatching percentage and survival rates, suggested an additional hallmark of this plant to be integrated in Aedes mosquito control. Ipomoea cairica deserved to be considered as one of the potential alternative sources for the new development of novel plant based insecticides in future.

  3. Effect of rainfall on the efficiency of herbicides applied in post-emergence on Ipomoea grandifolia

    OpenAIRE

    Souza, G.S.F. [UNESP; Martins, D; M.R.R. Pereira

    2013-01-01

    O objetivo deste trabalho foi avaliar a influência da chuva na eficiência de herbicidas aplicados em pós-emergência em plantas de Ipomoea grandifolia. As plantas de I. grandifolia foram cultivadas em vasos plásticos com capacidade de 2,5 L, em casa de vegetação, com uma planta por vaso. O delineamento experimental utilizado foi o inteiramente casualizado, com quatro repetições, com os tratamentos dispostos em um esquema fatorial 7x8 (sete tratamentos químicos e oito períodos para ocorrência d...

  4. 一种新的六倍体细胞类型水生薏苡的细胞遗传学鉴定%Cytogenetic Identification of a New Hexaploid Coix aquatica Cyto-type

    Institute of Scientific and Technical Information of China (English)

    韩永华; 李冬郁; 李英才; 薛跃规; 胡中立; 宋运淳

    2004-01-01

    通过醋酸洋红压片和荧光原位杂交技术(包括基因组原位杂交技术),确定在我国广西西南部地区广泛分布着的水生薏苡(Coix aquatica Roxb.)属于一种新的六倍体细胞类型.这种水生薏苡与已报道的几种水生薏苡细胞类型的染色体数目均不相同,它的染色体数目是2n=30,在减数分裂前期Ⅰ和中期Ⅰ的细胞中形成10个二价体和10个单价体.基因组原位杂交结果表明,这种水生薏苡的20条染色体与四倍体的薏苡(C.lacryma-jobi,2n=20)的基因组DNA是高度同源的.45S和5S rDNA分别杂交到这种水生薏苡的两条染色体上,其中各有一条染色体与薏苡中携带45S和5S rDNA杂交信号的染色体具有相同的形状和信号的分布状态.据此推测:四倍体的薏苡可能是这种新的水生薏苡细胞类型的一个亲本,它的另一个亲本可能是八倍体的水生薏苡(C.aquatica,2n=40),因为这种八倍体的水生薏苡在核型、植株形态及生长环境等方面与新的六倍体细胞类型的水生薏苡相似.%A new Coix aquatica Roxb. cyto-type, found in the southwest of Guangxi Zhuang Autonomous Region in China, was identified and analyzed by acetocarmine squashing and fluorescence in situ hybridization (FISH) technique including genomic in situ hybridization (GISH). This new C. aquatica cyto-type was different from other C. aquatica cyto-types reported in chromosome structure. It was 2n =30 in chromosome number, and ten bivalents plus ten univalents were formed in meiotic prophase and metaphase I. GISH results indicated that its 20 chromosomes were highly homologous to chromosomes of allotetraploid species C. lacryma-jobi L. (2n = 20). The 45S and 5S rDNA were respectively located on the two different chromosomes of the C. aquatica. One of chromosomes carrying 45S rDNA showed its chromosome shape and signal location in the C. aquatica as the same as that in C. lacryma-jobi. So did one of chromosomes carrying 5S r

  5. Ipomoeassin F, a new cytotoxic macrocyclic glycoresin from the leaves of Ipomoea squamosa from the Suriname rainforest.

    Science.gov (United States)

    Cao, Shugeng; Norris, Andrew; Wisse, Jan H; Miller, James S; Evans, Randy; Kingston, David G I

    2007-08-01

    A new cytotoxic macrocyclic glycoresin, ipomoeassin F (6), has been isolated from the leaves of Ipomoea squamosa. The structure was elucidated by the interpretation of spectral data. Compound 6 was strongly active in the A2780 (human ovarian cancer cell line) assay with an IC(50) value of 0.036 microM.

  6. Antinociceptive and anti-inflammatory activities of crude extracts of Ipomoea involucrata leaves in mice and rats

    Institute of Scientific and Technical Information of China (English)

    Uche Fidelia Ijeoma; Shorinwa Olusayo Aderonke; Okorie Ogbonna; Mgbahurike Amaka Augustina; Chijioke-Nwauche Ifeyinwa

    2011-01-01

    Objective: To investigate antinociceptive and anti-inflammatory activities of crude extract from Ipomoea involucrata leaves (Convolvulaceae) in mice and rats.Methods:The antinociceptive activity was tested using acetic acid-induced abdominal writhing test in mice. The anti-inflammatory activity was evaluated using egg albumin–induced oedema of rat paw.Results:Phytochemical screening showed the presence of alkaloids, flavonoids, saponins, terpenoids and tannin. At the doses of 25-100 mg/kg,Ipomoea involucrata exhibited dose-dependent and significant increase in pain threshold in acetic acid–induced writhing test of mice (P<0.05, student t-test) The administration ofIpomoea involucrata leaf extract (25-100 mg/kg) showed dose-dependent decreases in paw volume of egg albumin induced oedema in rats and a significant higher anti-inflammatory activity compared to the standard control (Aspirin). Conclusions: These results support the claims on the traditional use of the ofIpomoea involucrata leaves in the treatment of toothache, rheumatic pains and other inflammatory conditions. Studies on the isolation and structural elucidation of the active principle are still needed being carried out.

  7. Fatty acid profiles of Garuga floribunda, Ipomoea pes-caprae, Melanolepis multiglandulosa and Premna odorata seed oils

    Science.gov (United States)

    The fatty acid profiles of the seed oils of four species from four plant families for which no or only sparse information on the fatty acid profiles is available are reported. The five seed oils are Garuga floribunda of the Burseraceae family, Ipomoea pes-caprae of the Convolvulaceae family, Melanol...

  8. A Functional mathematical index for predicting effects of food processing on eight sweet potato(Ipomoea batatas)cultivars

    Science.gov (United States)

    In this paper we apply an improved functional mathematical index (FMI), modified from those presented in previous publications, to define the influence of different cooking processes of eight sweet potato (Ipomoea batatas) cultivars on composition of six bioactive phenolic compounds (flavonoids). Th...

  9. Identificación in silico de un grupo de secuencias ortólogas conservadas (COS de Ipomoea batatas

    Directory of Open Access Journals (Sweden)

    Christian Solís Calero

    2013-03-01

    Full Text Available En el presente trabajo se describe una serie de procedimientos bioinformáticos para la predicción de un grupo secuencias ortólogas conservadas (COS de Ipomoea batatas, así como la evaluación de su potencial utilidad para la generación de marcadores moleculares y estudios de diversidad en esta especie. Con ese propósito usando los programas BLAST X y TBLASTN se realizó una comparación reciproca por similaridad entre secuencias ESTs procedentes de librerías de cDNAs de Ipomoea batatas, propias o disponibles de modo público en la base de datos GenBank, con secuencias COS de Arabidopsis thaliana. La anotación funcional de las secuencias COS predichas en Ipomoea batatas se realizo usando los programas BLASTX, INTERPROSCAN y PSI-BLAST. Se obtuvieron en total 204 secuencias COS candidatos de Ipomoea batatas, siendo 16 secuencias provenientes de una librería generada a partir de raíces de reserva. Se evaluó de modo computacional el polimorfismo de las secuencias COS de raíces de reserva, obteniéndose SNPs en 8 secuencias, y secuencias repetidas en tandem en una de ellas.

  10. Bioaccumulation of metals and metalloids in medicinal plant Ipomoea pes-caprae from areas impacted by tsunami.

    Science.gov (United States)

    Kozak, Lidia; Kokociński, Mikołaj; Niedzielski, Przemysław; Lorenc, Stanisław

    2015-02-01

    Tsunami events may have an enormous impact on the functioning of aquatic and terrestrial ecosystems by altering various relationships with biotic components. Concentrations of acid-leachable fractions of heavy metals and metalloids in soils and plant samples from areas affected by the December 2004 tsunami in Thailand were determined. Ipomoea pes-caprae, a common plant species growing along the seashore of this region, and frequently used in folk medicine, was selected to assess the presence of selected elements. Elevated amounts of Cd, Pb, Zn, and As in soil samples, and Pb, Zn, As, Se, Cr, and Ni in plant samples were determined from the tsunami-impacted regions for comparison with reference locations. The flowers of Ipomoea pes-caprae contained the highest amounts of these metals, followed by its leaves, and stems. In addition, its bioaccumulation factor (BAF) supports this capability of high metal uptake by Ipomoea pes-caprae from the areas affected by the tsunami in comparison with a reference site. This uptake was followed by the translocation of these elements to the various plant components. The presence of these toxic metals in Ipomoea pes-caprae growing in contaminated soils should be a concern of those who use this plant for medicinal purposes. Further studies on the content of heavy metals and metalloids in this plant in relation to human health concerns are recommended. © 2014 SETAC.

  11. Comparative studies on plant range size: Linking reproductive and regenerative traits in two Ipomoea species

    Science.gov (United States)

    Astegiano, Julia; Funes, Guillermo; Galetto, Leonardo

    2010-09-01

    Reproductive and regenerative traits associated with colonization and persistence ability may determine plant range size. However, few comparative studies on plant distribution have assessed these traits simultaneously. Pollinator richness and frequency of visits, autonomous self-pollination ability, reproductive output (i.e., reproductive traits), seed bank strategy and seedling density (i.e., regenerative traits) were compared between the narrowly distributed Ipomoea rubriflora O'Donnell (Convolvulaceae) and its widespread congener Ipomoea purpurea (L.) Roth. The narrowly distributed species showed higher ecological specialization to pollinators and lower autonomous self-pollination ability. Frequency of visits, natural seed/ovule ratio and fruit set, and total fruit production did not differ between species. However, the number of seeds produced per fruit was lower in the narrowly distributed species, translating into lower total seed production per plant. Indeed, I. rubriflora formed smaller transient and persistent seed banks and showed lower seedling density than the widespread I. purpurea. These reproductive and regenerative trait results suggest that the narrowly distributed species may have lower colonization and persistence ability than its widespread congener. They further suggest that the negative effects of lower fecundity in the narrowly distributed species might persist in time through the long-lasting effects of total seed production on seed bank size, reducing the species' ability to buffered environmental stochasticity. However, other regenerative traits, such as seed size, and processes such as pre- and post-dispersal seed predation, might modulate the effects of plant fecundity on plant colonization and persistence ability and thus range size.

  12. Energy expenditure in relation to activity of lesser mouse deer (Tragulus javanicus).

    Science.gov (United States)

    Darlis; Abdullah, N; Liang, J B; Purwanto, B; Ho, Y W

    2001-11-01

    Heat production (HP) of male and female mouse deer during eating, standing and sitting was determined using the open circuit respiration chamber (RC). The time taken for similar activities was also determined in an outdoor enclosure (OD). The animals were fed kangkong (Ipomoea aquatica), sweet potato (Ipomoea batatas) and rabbit pellet ad libitum. Male mouse deer consumed more dry matter (DM), organic matter (OM) and gross energy (GE) than female. The time for each activity of male and female mouse deer kept in RC and OD was similar. The average time spent in RC and OD for both male and female, respectively, for sitting (956 and 896 min/day) was significantly (P<0.01) longer than standing (463 and 520 min/day) and eating (21 and 24 min/day). Heat production for male and female mouse deer, respectively, during eating was the highest (0.44 and 0.43 kJ/kg W(0.75)/min) followed by standing (0.37 and 0.33 kJ/kgW(0.75)/min) and sitting (0.26 and 0.26 kJ/kg W(0.75)/min). The difference in HP per min during standing between male and female was significant (P<0.05). The HP for 08.00-14.00 h and 14.00-20.00 h periods were higher than 20.00-02.00 h and 02.00-08.00 h periods. The overall HP for males during 08.00-14.00 h and 14.00-20.00 h periods were significantly (P<0.05) higher (114.8 and 119.2 kJ/kg W(0.75)) than female (107.5 and 110.4 kJ/kg W(0.75)), respectively.

  13. Analysis of SSR Information in EST Resource of Ipomoea batatas (L.) Lam.and Ipomoea nil (L.) Roth%甘薯与牵牛EST资源的SSR信息分析

    Institute of Scientific and Technical Information of China (English)

    兰孟焦; 吴问胜; 王瑞珍; 赵朝森; 赵现伟

    2013-01-01

    为挖掘番薯(Ipomoea)属EST-SSR资源,从NCBI数据库下载23406条甘薯(Ipomoea batatas(L.)Lam.) EST和62282条牵牛(Ipomoea nil(L.)Roth) EST,利用生物信息学软件预处理、去冗余、拼接后得到12812条无冗余的甘薯EST(6.70Mb)和28422条牵牛唯一序列(17.19 Mb).对这些序列进行SSR搜索,在甘薯上获得328个SSR位点,出现频率为2.56%;牵牛上筛选到962个SSR位点,出现频率为3.38%.甘薯和牵牛EST-SSR具有多个共同特征:在SSR位点中,主要是二核苷酸重复类型,其次是三核苷酸重复;在二核苷酸重复中,出现最多的重复基序为AG/CT,其次是AT/AT;在三核苷酸重复中,主要基序是AAG/CCT; SSR位点的长度主要集中在20~22 bp.结果表明,搜索出的EST-SSR重复基序类型丰富、多态性潜能高,具有较高的开发和利用价值.%To excavate the EST-SSR resourses of lpomoea,12812 non-redundant ESTs with the total length about 6.70 Mb were obtained by assembling 23406 ESTs from Ipomoea batatas (L.) Lam.in NCBI.With the same way,28422 unique sequences covering 17.19 Mb were generated from Ipomoea nil (L.) Roth.A total of 328 SSR loci for Ipomoea batatas with the the frequency of 2.56% and 962 SSR loci for Ipomoea nil with the frequency of 3.38% were identified by MISA.The EST-SSRs from Ipomoea batatas and Ipomoea nil had many common features.Among all the identified SSRs,dinucleotide repeats were dominant motifs and then were trinucleotide repeats.For dinucleotide repeats,AG/CT was the major motif,followed by AT/AT.And AAG/CCT was the most frequent motif among the trinucleotide repeats.The length of EST-SSRs was mainly distributed among 20-22 bp.The results indicated that these EST-SSRs with abundant repeat motif types and high potential polymorphism had high value for exploitation and utilization.

  14. Anatomical-morphological changes in Glyceria aquatica (L. Wahlb. and Phalaris arundinacea L. growing in the zone inundated by the Kwiecko lake

    Directory of Open Access Journals (Sweden)

    Eugeniusz R. Śpiewakowski

    2014-01-01

    Full Text Available Morphological-anatomical changes are described in the shoots of Glyceria aquatica and Phalaris arundinacea. These grasses encroach upon the zone inundated by the lake Kwiecko which is the lower reservoir of the Hydroelectric Plant Żydowo. The interaction of habitat and anthropogenic factors causes disturbances in the physiology of reproduction in the above mentioned plants and produces changes in their external and internal structure. Both species form in the zone inundated by the lake vegetative shoots exclusively, with profuse aerial tillering at the apical meristems. The morphological changes consist in shortening of the shoots and multiplication of the internodes. In the internal structure a narrowing of the ring of sclerenchyma, a shift of the vascular bundles into the sclerenchyma and an increase of its layer and air channels are observed.

  15. Chemical composition of the essential oil from Corsican Mentha aquatica--combined analysis by GC(RI), GC-MS and 13C NMR spectroscopy.

    Science.gov (United States)

    Sutour, Sylvain; Tomi, Félix; Bradesi, Pascale; Casanova, Joseph

    2011-10-01

    The essential oil (EO) of M. aquatica L. growing wild in Corsica was isolated by dry vapor distillation and submitted to combined analysis by column chromatography over silica gel, GC(RI), GC-MS and 13C NMR spectroscopy. The composition was dominated byoxygenated monoterpenes and characterized by the occurrence of menthofuran (50.7%) as the major component. In parallel, seven laboratory-distilled oil samples isolated from individual plants collected in Corsica were analyzed by GC(RI) and 13C NMR spectroscopy. Onlyquantitative differences were observed between the samples. Beside the usual terpenes, various p-menthane lactones (mintlactone, isomintlactone, hydroxymintlactone, menthofurolactone and epimenthofurolactone) have been identified in all the oil samples.

  16. Assessment of reproductive capacity of estuarine plants Butomus umbellatus L. and Alisma plantago-aquatica L. from radioactively contaminated flood plains

    Energy Technology Data Exchange (ETDEWEB)

    Kaneva, A.V.; Majstrenko, T.A.; Rachkova, N.G.; Belykh, E.S.; Zainullin, V.G. [Institute of Biology, Komi Scientific Center, Ural Division of RAS, Syktyvkar, 167982 (Russian Federation)

    2014-07-01

    It is known that the vegetation, along with the climatic conditions and soil type, is one of the key components of terrestrial ecosystems. They are also first to respond to the substrate contamination with radionuclides, metals and organic substances. Biological effects observed in natural plant populations are associated with both presence of mobile compounds of pollutants in abiotic components of the ecosystem and their role in the metabolism of the plant. The goal of the study was to assess the impact of water and sediment contamination with artificial radionuclides and toxic non-radioactive compounds, on the reproductive capacity of estuarial plants using seed germination. Contaminated sites are located in flood plain of the Techa River (Chelabinsk region, Russia) between Muslumovo and Brodokalmak settlements. Radioactive contamination of the territory resulted from increased specific activities of {sup 137}Cs and {sup 90}Sr in water and sediments due to the accidents on the Mayak Production Association. Reference sites were chosen in the flood plains of Brusianka and Sysert' rivers (Sverdlovsk region, Russia). Reference sites are located out of the Eastern Ural Radioactive Trace. Seeds of Butomus umbellatus L. and Alisma plantago-aquatica L., which are common estuarine plant communities in this area, were collected. Specific activities of dose-forming radionuclides in the Techa river water vary from 120 up to 200 mBq/l for {sup 137}Cs and from 26 up to 45 mBq/l for {sup 90}Sr; and in sediments 720-10150 Bq/kg and 600-1500 Bq/kg for {sup 137}Cs and {sup 90}Sr correspondingly. Specific activities of {sup 137}Cs and {sup 90}Sr in water and sediments of both reference rivers do not exceed global fallout levels. B. umbellatus seeds germination was low for plant populations of both reference and contaminated sites. However, a significant (p<0.01) difference was found - the value was higher for reference populations (17.4 ±3.5 %) as compared with the ones from

  17. Removal of mercury(II) ions in aqueous solution using the peel biomass of Pachira aquatica Aubl: kinetics and adsorption equilibrium studies.

    Science.gov (United States)

    Santana, Andrea J; dos Santos, Walter N L; Silva, Laiana O B; das Virgens, Cesário F

    2016-05-01

    Mercury is a highly toxic substance that is a health hazard to humans. This study aims to investigate powders obtained from the peel of the fruit of Pachira aquatica Aubl, in its in natura and/or acidified form, as an adsorbent for the removal of mercury ions in aqueous solution. The materials were characterized by Fourier transform infrared spectroscopy and thermogravimetric analysis. The infrared spectra showed bands corresponding to the axial deformation of carbonyls from carboxylic acids, the most important functional group responsible for fixing the metal species to the adsorbent material. The thermograms displayed mass losses related to the decomposition of three major components, i.e., hemicellulose, cellulose, and lignin. The adsorption process was evaluated using cold-vapor atomic fluorescence spectrometry (CV AFS) and cold-vapor atomic absorption spectrometry (CV AAS). Three isotherm models were employed. The adsorption isotherm model, Langmuir-Freundlich, best represented the adsorption process, and the maximum adsorption capacity was predicted to be 0.71 and 0.58 mg g(-1) at 25 °C in nature and acidified, respectively. Adsorption efficiencies were further tested on real aqueous wastewater samples, and removal of Hg(II) was recorded as 69.6 % for biomass acidified and 76.3 % for biomass in nature. Results obtained from sorption experiments on real aqueous wastewater samples revealed that recovery of the target metal ions was very satisfactory. The pseudo-second-order model showed the best correlation to the experimental data. The current findings showed that the investigated materials are potential adsorbents for mercury(II) ion removal in aqueous solution, with acidified P. aquatica Aubl being the most efficient adsorbent.

  18. Antifungal activity and isomerization of octadecyl p-coumarates from Ipomoea carnea subsp. fistulosa.

    Science.gov (United States)

    Nidiry, Eugene Sebastian J; Ganeshan, Girija; Lokesha, Ankanahalli N

    2011-12-01

    Bioassay monitored HPLC assisted isolation and purification of the chief antifungal fraction of the leaves of Ipomoea carnea subsp. fistulosa (Convulvulaceae) were achieved using Colletotrichum gloeosporioides and Cladosporium cucumerinum as test organisms. The activity of the purified fraction was further confirmed by the dose dependent inhibition of the spore germination of Alternaria alternata and A. porri. The active fraction was identified as a mixture of (E)-octadecyl p-coumarate and (Z)-octadecyl p-coumarate. The two isomers were detected on an HPLC column with substantially different retention times, but once eluted from the column, one form was partly converted to the other in daylight. Conclusive evidence for the structures and their isomerization were obtained from the HPLC behavior, IR, UV, HRESIMS, CIMS and and NMR spectral data. Important 1H NMR and 13C NMR signals could be separately assigned for the isomers using 2D NMR techniques.

  19. Phyotoxicity of diesel soil contamination on the germination of Lactuca sativa and Ipomoea batatas.

    Science.gov (United States)

    Fatokun, Kayode; Lewu, Francis Bayo; Zharare, Godfrey Elijah

    2015-11-01

    Phytotoxic effect of diesel contaminated soil on germination rate of Lactuca sativa and Ipomoea batatas, at two concentrations ranges (0-6ml and 0-30ml), were investigated and compared. Diesel soil contamination was simulated and soil samples were taken from contaminated soil at 1, 5,10, 15, 25, 50, 75 and 100 days should be after planting. The result showed that in both plant species, diesel inhibited germination in a concentration dependent manner, Also, the influence of diesel contamination diminished with increased time duration; suggesting possible reduction in diesel toxicity over time. However, germination of lettuce was significant and negatively correlated (r2 = -0.941) with diesel contamination as compared to sweet potato (r2 = -0.638).Critical concentration of diesel in relation to seed germination of L. sativa was lower than vegetative germination of I. batatas, indicating that germination of I. batatas was less sensitive to diesel contamination as compared to L. sativa.

  20. Ipomoea dasysperma seed gum: an effective natural coagulant for the decolorization of textile dye solutions.

    Science.gov (United States)

    Sanghi, Rashmi; Bhattacharya, Bani; Dixit, Awantika; Singh, Vandana

    2006-10-01

    An investigation of dye decolorization from synthetic dye solutions using the non-ionic, water-soluble, high molecular weight seed gums Ipomoea dasysperma and guar gum as coagulants was undertaken. The use of galactomannans derived from plants in this system presents a sustainable method of textile effluent treatment. These natural coagulants extracted from plants proved to be workable alternatives to conventional coagulants like polyaluminum chloride, as they are biodegradable, safe to human health, are cost effective when compared to imported chemicals and have a wider effective dosage range for flocculation of various colloidal suspensions. Coagulant dose and coagulation pH are important factors influencing the mechanism of coagulation. Also the type and chemical structure of the dye plays an important role in the coagulation process. The seed gums alone were found to be effective for decolorization of direct dye and in combination with PAC their coagulation efficiency was well extended even for reactive and acid dyes.

  1. Chemical Constituents from the Tubers of Ipomoea batatas%甘薯的化学成分

    Institute of Scientific and Technical Information of China (English)

    尹永芹; 孔令义

    2008-01-01

    目的:对甘薯(Ipomoea batatas)的化学成分进行研究.方法:运用多种层析方法进行分离纯化,通过波谱和化学方法确定化合物的结构.结果:分离鉴定了5个化合物,即乙酰-β-香树醇(1)、表木栓醇(2)、咖啡酸乙酯(3)、咖啡酸(4)、simonin Ⅳ(5).结论:5个化合物为首次从该植物中分离得到,其中simonin Ⅳ(5)为首次从甘薯中分离得到的树脂糖苷类化合物.

  2. Development of Randomly Amplified Polymorphic DNA Based SCAR Marker for Identification of Ipomoea mauritiana Jacq (Convolvulaceae

    Directory of Open Access Journals (Sweden)

    Kambiranda Devaiah

    2011-01-01

    Full Text Available Vidari is an Ayurvedic herbal drug used as aphrodisiac, galactagogue and is also used in the preparation of Chyavanaprash. Tubers of Ipomoea mauritiana Jacq. (Convolvulaceae, Pueraria tuberosa (Roxb. ex Willd. DC (Fabaceae, Adenia hondala (Gaertn. de Wilde (Passifloraceae and pith of Cycas circinalis L. (Cycadaceae are all traded in the name of Vidari, creating issues of botanical authenticity of the Ayurvedic raw drug. DNA-based markers have been developed to distinguish I. mauritiana from the other Vidari candidates. A putative 600-bp polymorphic sequence, specific to I. mauritiana was identified using randomly amplified polymorphic DNA (RAPD technique. Furthermore, sequence characterized amplified region (SCAR primers (IM1F and IM1R were designed from the unique RAPD amplicon. The SCAR primers produced a specific 323-bp amplicon in authentic I. mauritiana and not in the allied species.

  3. Photosynthetic efficiency and water consumption of Ipomoea triloba after herbicide application

    OpenAIRE

    Araldi,R; Girotto,M; E.D Velini; Gomes,G.L.G.C; Jasper, S. P.; C.A Carbonari; M.L.B. Trindade

    2012-01-01

    Este trabalho foi desenvolvido com o objetivo de avaliar a fluorescência através da taxa de transporte de elétrons, consumo de água e intoxicação de plantas de Ipomoea triloba após aplicação de quatro herbicidas de diferentes mecanismos de ação. Os herbicidas aplicados foram: glyphosate, haloxyfop-methyl, diuron e amicarbazone. A aplicação foi feita com auxílio de um pulverizador estacionário instalado em laboratório; após a aplicação dos tratamentos, as plantas foram mantidas em casa de vege...

  4. Sensorial, physical and chemical evaluation of bio-fortified Ipomoea batatas

    Directory of Open Access Journals (Sweden)

    Mara N.G. Santos

    2014-08-01

    Full Text Available Context: Bio-fortified sweet potato, Ipomoea batatas (L. Lamarck beta-carotene rich, has been included in the most vulnerable population’s diet with the purpose of reducing health disturbances associated with hipovitaminosis. Aims:To evaluate a physical and chemical analysis of total carotenes and beta-carotene and to determine the antioxidant potential as well as to carry out a sensorial analysis of the bio-fortified Ipomoea batatas (L. Lamarck (BDB in nature and processed. Methods: BDB was processed as sweet (in natura, paste and syrup and physicochemical characteristics were compared, total carotenoids, beta-carotene, antioxidant activity (DPPH and microbiological and sensory analyses were performed. Results:The analysis of total carotenoids, beta-carotene and anti-oxidant potential showed the following results: BDB in nature – 11.81 mg/100 g/440.5 mg/100 g/26.30%; sweet paste – 0.61 mg/100 g/53.5 mg/100 g/53.40% and savored syrup – 0.85 mg/100 g/46.0 mg/100 g/14.30%. The methods of conservation avoided the coliforms growth at 35 and 45°C, Staphylococcus coagulasa positivo, Bacillus cereus and Salmonella in all elaborated candies. The sensorial analysis didn’t detect significant differences among the syrup or paste. Conclusions: The conservation methods have an important function keeping the physical, chemical characteristics and sensorial of BDB, although they can influence in their chemical and nutritional composition, mainly in relation to the quantity of total carotenoids and beta-carotene.

  5. Changes in electrophoretic profiles of Ipomoea batatas (sweet potato induced by gamma radiation

    Directory of Open Access Journals (Sweden)

    Celso Luiz Salgueiro Lage

    2002-06-01

    Full Text Available The ability of nodal segments of Ipomoea batatas to differentiate shoots and roots was evaluated after gamma irradiation. Shoot differentiation was less sensitive to irradiation than roots. However, at 90 Gy, no shoot was able to regenerate a new plant; in contrast 76 % of the roots from irradiated nodal segments continued to grow. The gamma radiation also induced changes in electrophoretic profiles of peroxidases of storage roots. Plants originated from irradiated storage roots presented changes in leaf peroxidase profiles very similar to those produced by leaves directly irradiated. The peroxidase profile of absorbent roots from irradiated storage roots was different from that obtained from directly irradiated absorbent roots.A capacidade de Ipomoea batatas diferenciar parte aérea e raízes foi avaliada após irradiação com raios gama. A diferenciação da parte aérea foi menos sensível que a das raízes. Contudo, na dose de 90 Gy nenhum broto diferenciado regenerou planta, enquanto 76 % das raízes diferenciadas dos segmentos nodais irradiados manteve o crescimento. A radiação também induziu mudanças no perfil elotroforético de isoperoxidases. Plantas originadas de raízes tuberosas irradiadas apresentaram alteração no perfil de isoperoxidases foliares semelhantes ao perfil de folhas diretamente irradiadas. O perfil de isoperoxidases de raízes absorventes irradiadas diretamente não apresentou o mesmo padrão do perfil das raízes absorventes desenvolvidas de raízes tuberosas irradiadas.

  6. Chemical constituents of essential oils of Tephrosia purpurea and Ipomoea carnea and their repellent activity against Odoiporus longicollis

    OpenAIRE

    Sahayaraj Kitherian; Kombiah Poolpandi; Dikshit Anand K.; Rathi Martin J.

    2015-01-01

    Chemical constituents of essential oils (EOs) obtained from stem and root of Tephrosia purpurea (Linn.) Pers. and Ipomoea carnea Jacq. were investigated by Gas Chromatography-Mass Spectrometry (GC-MS). Total lipid and oil content was high in the stem than the root of T. purpurea and I. carnea. Essential oils extracted from the stem and root of T. purpurea and I. carnea showed 9 and 8 compounds respectively. Hexadecanoic acid was found to be the principal co...

  7. Evaluation of the effect of an organic extract obtained from Ipomoea alba L. on experimental periodontitis in rats

    OpenAIRE

    Guilherme Emerson Barrella; Ivana Barbosa Suffredini; Fernanda Vieira Ribeiro; Fabiano Ribeiro Cirano; Suzana Peres Pimentel

    2012-01-01

    The aim of this study was to evaluate the effect of an organic extract obtained from Ipomoea alba L. (Convolvulaceae or OE 1493), on experimental periodontal disease in rats. Periodontitis was induced in thirty six Wistar rats: a first mandibular molar was randomly assigned to receive a ligature, whereas the contralateral molar was left unligated. Animals were randomly assigned to two groups and treated topically, three times a day, for 11 days, as follows: Control Group - vehicle-treated (n ...

  8. Sedative, Anxiolytic and Anticonvulsant effects of different extracts from the leaves of Ipomoea Carnea in experimental Animals

    OpenAIRE

    Susanta Kumar Rout; Durga Madhab Kar

    2013-01-01

    The objective of this study is to investigate the sedative, anxiolytic and anticonvulsant activities of different leaf extracts of Ipomoea carnea. Materials and methods: The sedative effect of the different leaf extracts at dose level 100, 200 and 400 mg/kg was evaluated in mice and rats using phenobarbitone induced sleeping time and hole board models. Its anxiolytic effect was evaluated using the Evaluated Plus Maze (EPM) and the Y maze (YM) methods. The anticonvulsant activity was evaluated...

  9. Métodos de superação de dormência em sementes de Ipomoea e Merremia Dormancy breaking methods in Ipomoea and Merremia seeds

    Directory of Open Access Journals (Sweden)

    A.A.P.M. Azania

    2003-08-01

    Full Text Available Este trabalho objetivou avaliar os efeitos de métodos de superação de dormência sobre a germinação das sementes de Ipomoea grandifolia, I. hederifolia, I. nil, I. quamoclit, Merremia aegyptia e M. cissoides. Os tratamentos consistiram em ácido sulfúrico concentrado, nitrato de potássio, água quente e calor seco (50 ºC, lixa e fogo. Constatou-se que o ácido sulfúrico promoveu aumento na germinação das sementes de I. grandifolia (58, 37, 22 e 34%, I. hederifolia (76, 49, 82 e 55%, I. quamoclit (43, 33, 66 e 35%, I. nil (69, 79, 72 e 62%, M. cissoides (8, 19, 35 e 57% e M. aegyptia (24, 64, 56 e 63% após períodos de imersão de 5, 10, 15 e 20 minutos, respectivamente. A água (20 e 40 minutos, o calor seco (20 e 40 minutos e o fogo melhoraram a germinação de I. grandifolia (68, 59, 62, 67 e 59%, M. cissoides (50, 52, 18, 25 e 46% e M. aegyptia (54, 47, 21, 21 e 45%, respectivamente. O calor seco de 20 e 40 minutos melhorou a germinação de I. nil (49 e 36%, e o de 40 minutos, a de I. hederifolia (70%. O uso de nitrato de potássio e lixa não proporcionou melhoria na germinação das sementes.This research aimed to evaluate the effects of dormancy breaking methods on germination of Ipomoea grandifolia, I. hederifolia, I. nil, I. quamoclit, Merremia aegyptia and M. cissoides seeds. The treatments consisted of concentrated sulfuric acid, potassium nitrate, and mechanical and physical scarification for fire, water and dry heat at 50 ºC. The sulfuric acid increased seed germination of I. grandifolia (58, 37, 22 and 34 %, I. hederifolia (76, 49, 82 and 55%, I. quamoclit (43, 33, 66 and 35%, I. nil (69, 79, 72 e 62%, M. cissoides (8, 19, 35 and 57% and M. aegyptia (24, 64, 56 and 63% after 5, 10, 15 and 20 min, respectively. Water (20 and 40 min, dry heat (20 and 40 min and fire increased germination of I. grandifolia (68, 59, 62, 67 and 59%, M. cissoides (50, 52, 18, 25 and 46% and M. aegyptia (54, 47, 21, 21 and 45%, respectively

  10. Pneumonia intersticial em bovinos associada à ingestão de batata-doce (Ipomoea batatas mofada Interstitial pneumonia in cattle fed moldy sweet potatoes (Ipomoea batatas

    Directory of Open Access Journals (Sweden)

    Rafael A. Fighera

    2003-12-01

    Full Text Available Uma doença respiratória foi diagnosticada em cinco dentre 23 bovinos (21,7% após terem sido alimentados com batata-doce (Ipomoea batatas mofada em uma pequena propriedade rural em São Vicente do Sul, Rio Grande do Sul, Brasil. Três dos cinco bovinos afetados morreram espontaneamente, e o quarto foi sacrificado para necropsia quando mostrava sinais clínicos respiratórios avançados. A manifestação clínica iniciara cerca de 24 horas após a ingestão das batatas-doces e a evolução clínica foi de 1 a 4 dias. Os sinais clínicos incluíam dispnéia (respiração laboriosa e abdominal, taquipnéia, pescoço estendido com cabeça baixa e dilatação ritmada das narinas. Dois bovinos foram necropsiados. Os achados de necropsia incluíam pulmões distendidos, pálidos e de consistência borrachenta, que não colapsavam quando o tórax era aberto; enfisema e edema acentuados eram evidentes no pulmão. Os linfonodos e o baço apresentavam alterações características de hiperplasia linfóide. Histologica-mente, as lesões eram típicas de pneumonia intersticial. Os septos alveolares estavam espessados por fibroblastos e células inflamatórias, havia hipertrofia e hiperplasia de pneumócitos tipo II; os septos interlobulares estavam distendidos por edema e enfisema. A cultura de amostras das batatas-doces mofadas produziu Fusarium solani e F. oxysporum.Cases of respiratory disease were diagnosed in five out of 23 cattle (21.7% after they were fed moldy damaged sweet potatoes (Ipomoea batatas on a small farm in the county of São Vicente do Sul, state of Rio Grande do Sul, Brazil. Of those five cattle, three died spontaneously and another one was euthanatized for necropsy while showing advanced respiratory clinical signs. The disease manifested itself approximately 24 hours after the ingestion of the sweet potatoes and lasted from 1 to 4 days. Clinical signs included dyspnea (labored breathing and abdominal respiration, tachypnea, extended

  11. The effect of Ipomoea reptans poir ethanolic extract on the histopathological parameters of pancreas in streptozotocin-induced diabetic rats

    Science.gov (United States)

    Hayati, Farida; Widyarini, Sitarina; Lanova, Lulung; Wijayanti, Marsih

    2017-03-01

    The aim of this study was to investigate the effect of ethanolic extract of Ipomoea reptans Poir in male Wistar rats on the histopathological parameters of the pancreas. The rats (N=30) were divided into six groups, each consisting of five rats. The treatment groups were divided into: group I as the normal group fed ad libitum during the research, group II as the positive control administered glibenclamide 0.09 mg/200g BW, group III as the negative control given aquadest, and group IV to VI given ethanolic extract of Ipomoea reptans Poir as much as 200 mg/KgBW, 400 mg/KgBW and 600 mg/KgBW respectively. The study of antidiabetic effect was undertaken by oral administration for 21 days. On the 21st day, all the rats were dissected to prepare histopathological preparates through Gomori's chrome alum hematoxylin-phloxine staining method. The histopathological study showed that the ethanol extract of Ipomoea reptans Poir at a dose of 200 mg/KgBW and 400mg/KgBW had an antidiabetic activity through protection of pancreatic β-cell from damage in male Wistar rats induced by streptozotocin.

  12. Study on chemical constituents from Ipomoea Pes-caprae(L.)Sweet Ⅱ%厚藤(Ipomoea Pes-caprae(L.)Sweet)化学成分研究Ⅱ

    Institute of Scientific and Technical Information of China (English)

    王清吉; 王友绍; 何磊; 娄治平; 张偲

    2010-01-01

    目的 研究药用滨海植物厚藤(Ipomoea Pes-caprae(L.)Sweet)的化学成分.方法 采用硅胶柱色谱和Sephadex LH-20凝胶柱色谱从厚藤的乙酸乙酯提取物中分离到8个化合物,经理化常数和波谱分析鉴定了这8个化合物的结构.结果 它们分别是十九烷酸(I)、阿魏酸(Ⅱ)、咖啡酸(Ⅲ)、尼泊金甲酯(Ⅳ)、5,7-二羟基-4'-甲氧黄酮(V)、5,7-二羟基-4'-甲氧黄酮醇(Ⅵ)、4'-羟基-3,5,7-三甲氧黄酮(Ⅶ)和7,3',4'-三羟基-5-甲氧黄酮醇(Ⅷ).结论 其中,阿魏酸、咖啡酸、尼泊金甲酯、5,7-二羟基-4'-甲氧黄酮、5,7-二羟基-4'-甲氧黄酮醇、4'-羟基-3,5,7-三甲氧黄酮和7,3',4'-三羟基-5-甲氧黄酮醇为首次从该植物中分离得到.

  13. IPOMOEA BATATAS)

    African Journals Online (AJOL)

    eobe

    petroleum wastes, leaking storage tanks and accidental ... health, surface and ground water quality, the nature and viability of ..... Biotechnology, Vol. 24, Number (2-3) ... pollutants in Europe”. .... “Removal of lead ion from industrial waste water.

  14. Characterization and development of EST-derived SSR markers in cultivated sweetpotato (Ipomoea batatas

    Directory of Open Access Journals (Sweden)

    Li Yujun

    2011-10-01

    Full Text Available Abstract Background Currently there exists a limited availability of genetic marker resources in sweetpotato (Ipomoea batatas, which is hindering genetic research in this species. It is necessary to develop more molecular markers for potential use in sweetpotato genetic research. With the newly developed next generation sequencing technology, large amount of transcribed sequences of sweetpotato have been generated and are available for identifying SSR markers by data mining. Results In this study, we investigated 181,615 ESTs for the identification and development of SSR markers. In total, 8,294 SSRs were identified from 7,163 SSR-containing unique ESTs. On an average, one SSR was found per 7.1 kb of EST sequence with tri-nucleotide motifs (42.9% being the most abundant followed by di- (41.2%, tetra- (9.2%, penta- (3.7% and hexa-nucleotide (3.1% repeat types. The top five motifs included AG/CT (26.9%, AAG/CTT (13.5%, AT/TA (10.6%, CCG/CGG (5.8% and AAT/ATT (4.5%. After removing possible duplicate of published EST-SSRs of sweetpotato, a total of non-repeat 7,958 SSR motifs were identified. Based on these SSR-containing sequences, 1,060 pairs of high-quality SSR primers were designed and used for validation of the amplification and assessment of the polymorphism between two parents of one mapping population (E Shu 3 Hao and Guang 2k-30 and eight accessions of cultivated sweetpotatoes. The results showed that 816 primer pairs could yield reproducible and strong amplification products, of which 195 (23.9% and 342 (41.9% primer pairs exhibited polymorphism between E Shu 3 Hao and Guang 2k-30 and among the 8 cultivated sweetpotatoes, respectively. Conclusion This study gives an insight into the frequency, type and distribution of sweetpotato EST-SSRs and demonstrates successful development of EST-SSR markers in cultivated sweetpotato. These EST-SSR markers could enrich the current resource of molecular markers for the sweetpotato community and would

  15. Aeromonas aquatica sp. nov., Aeromonas finlandiensis sp. nov. and Aeromonas lacus sp. nov. isolated from Finnish waters associated with cyanobacterial blooms.

    Science.gov (United States)

    Beaz-Hidalgo, R; Latif-Eugenín, F; Hossain, M J; Berg, K; Niemi, R M; Rapala, J; Lyra, C; Liles, M R; Figueras, M J

    2015-05-01

    Three groups of Aeromonas strains isolated from Finland lakes experiencing cyanobacterial blooms could not be assigned to any known species of this genus on the basis of 16S rRNA and rpoD gene sequences. The Multilocus Phylogenetic Analysis (MLPA) of the concatenated sequence of seven genes (gyrB, rpoD, recA, dnaJ, gyrA, dnaX and atpD; 4093bp) showed that the three groups of strains did not cluster with any known Aeromonas spp. and formed three independent lineages. This was confirmed by performing the analysis with their closest relatives using 15 genes (the latter 7 and cpn60, dnaK, gltA, mdh, radA, rpoB, tsf, zipA; 8751bp). Furthermore, ANI results between the genomes of the type strains of the three potential new species and those of their close relatives were all Aeromonas species on the basis of several phenotypic characters. This polyphasic study revealed that the 3 groups of strains represent 3 novel Aeromonas species for which the names Aeromonas aquatica sp. nov. (type strain AE235T=CECT 8025T=LMG 26712T), Aeromonas finlandiensis sp. nov. (type strain 4287DT=CECT 8028T=LMG 26709T) and Aeromonas lacus sp. nov. (type strain AE122T=CECT 8024T=LMG 26710T) are proposed.

  16. Germination and emergence of Stachytarpheta cayennensis and Ipomoea asarifolia Germinação e emergência de Ipomea asarifolia e Stachytarpheta cayennensis

    Directory of Open Access Journals (Sweden)

    M.B. Dias Filho

    1996-01-01

    Full Text Available Understanding how weed seed germination and emergence respond to environmental factors is critical to determining their adaptive capabilities and potential for infestations, and could also aid in the development of effective control practices. Germination of Ipomoea asarifolia (Desr. Roem. & Schultz and Stachytarpheta cayennensis (Rich Vahl. decreased linearly with decreasing osmotic potentials. Also, increasing osmotic stress delayed germination of Ipomoea more than that of Stachytarpheta. Ipomoea germination was insensitive to light, while Stachytarpheta showed a positive photoblastic behavior. Nitrate had a negative effect on germination of Ipomoea seed under both light and dark conditions but stimulated dark germination of Stachytarpheta. Ipomoea emergence was not significantly affected by planting depth. However, for Stachytarpheta emergence was restrited to seeds planted at the soil surface. Emergence of Ipomoea seedlings from greater than 6cm significantly decreased the amount of biomass allocated to roots, while biomass allocated to leaves was decreased for seedlings that emerged from depths greater than 2cm. These germination and emergence responses are discussed in relation to their ecological implications and to weed control strategies.O entendimento de como a germinação e emergência de plantas daninhas respondem a fatores ambientais é de grande importância para a determinação da capacidade de adaptação e potencial de infestação, podendo ainda auxiliar no desenvolvimento de práticas de controle eficazes. A germinação de Ipomoea asarifolia (Desr. Roem. & Schultz e Stachytarpheta cayennensis (Rich Vahl. Diminuiram linearmente com o decréscimo do potencial osmótico do meio de germinação, sendo que o aumento do estresse osmótico atrasou com maior intensidade a germinação de Ipomoea do que a de Stachytarpheta. A germinação de Ipomoea foi insensível à luz; já a de Stachytarpheta mostrou ter um comportamento fotobl

  17. In vivo wound healing and antiulcer properties of white sweet potato (Ipomoea batatas

    Directory of Open Access Journals (Sweden)

    Daniele Hermes

    2013-07-01

    Full Text Available The potential of tuber flour of Ipomoea batatas (L. Lam. cv. Brazlândia Branca (white sweet potato as wound healing and antiulcerogenic agent was investigated in vivo in animal model. Excision on the back of Wistar rats was performed to induce wounds that were topically treated with Beeler’s base containing tuber flour of white sweet potato at 2.5%. Number of cells undergoing metaphase and the degree of tissue re-epithelialization were investigated 4, 7 and 10 days post-treatment. The protective effect of aqueous suspension of tuber flour (75 and 100 mg/kg animal weight on gastric mucosa of Wistar rats was also studied by using the ethanol-induced ulceration model. Ointment based on white sweet potato at 2.5% effectively triggered the healing of cutaneous wound as attested by the increased number of cells undergoing metaphase and tissue re-epithelialization regardless the time of wound treatment. Tuber flour potentially prevented ethanol-induced gastric ulceration by suppressing edema formation and partly protecting gastric mucosa wrinkles. Crude extracts also exhibited potential as free radical scavengers. The results from animal model experiments indicate the potential of tuber flour of white sweet potato to heal wounds.

  18. Influence of pollination specialization and breeding system on floral integration and phenotypic variation in Ipomoea.

    Science.gov (United States)

    Rosas-Guerrero, Víctor; Quesada, Mauricio; Armbruster, W Scott; Pérez-Barrales, Rocío; Smith, Stacey DeWitt

    2011-02-01

    Natural selection should reduce phenotypic variation and increase integration of floral traits involved in placement of pollen grains on stigmas. In this study, we examine the role of pollinators and breeding system on the evolution of floral traits by comparing the patterns of floral phenotypic variances and covariances in 20 Ipomoea species that differ in their level of pollination specialization and pollinator dependence incorporating phylogenetic relatedness. Plants with specialized pollination (i.e., those pollinated by one functional group or by few morphospecies) displayed less phenotypic variation and greater floral integration than generalist plants. Self-compatible species also displayed greater floral integration than self-incompatible species. Floral traits involved in pollen placement and pick up showed less variation and greater integration than floral traits involved in pollinator attraction. Analytical models indicate that both breeding system and the number of morphospecies had significant effects on floral integration patterns although only differences in the former were significant after accounting for phylogeny. These results suggest that specialist/self-compatible plants experience more consistent selection on floral traits than generalist/self-incompatible plants. Furthermore, pollinators and breeding system promote integration of floral traits involved in pollen placement and pick up rather than integration of the whole flower. © 2010 The Author(s). Evolution© 2010 The Society for the Study of Evolution.

  19. Microbial deterioration of white variety sweet potato (Ipomoea batatas under different storage structures

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    Wisdom Amoa-Awua

    2010-01-01

    Full Text Available Post-harvest protection of white variety sweet potato (Ipomoea batatas has been dealt with rarely in the past, although it is comparatively easy to grow and has high consumer acceptability in Ghana. Microbial deterioration of sweet potato roots stored in three different storage structures was studied. The sweet potato roots initially cured for 7 and 14 days were stored in traditional, pit, and clamp storage structures for a maximum of 28 days. For the 7 days-cured sweet potato roots, the bac­teria population in the three different storage structures increased by 1.2-2.3 log cfu/g whereas for the 14 days-cured roots, the bacteria population was 0.1-1.0 log cfu/g within 28 days of storage. The fungal population in the 14 days-cured sweet potato roots was higher than in the 7 days-cured sweet potato roots by 0.6-1.6 log cfu/g for 28 days of storage. For both the 7 and 14 days-cured sweet potato roots, the sweet potato roots stored for 28 days in the three different storage structures had a higher microbial count compared to the sweet potato roots stored for 14 days. Aspergillus flavus was the most dominant fungal species occurring in all of the three different storage structures followed by A. niger, Rhizopus stolonifer, Tricho-derma viride, Fusarium oxysporum, Penicillium digitatum, Cladosporium herbarum,and Aspergillus ochraceus, in that order.

  20. EVALUATION OF SUBLETHAL EFFECTS OF Ipomoea cairica LINN. EXTRACT ON LIFE HISTORY TRAITS OF DENGUE VECTORS

    Science.gov (United States)

    ZUHARAH, Wan Fatma; AHBIRAMI, Rattanam; DIENG, Hamady; THIAGALETCHUMI, Maniam; FADZLY, Nik

    2016-01-01

    Plant derived insecticides have considerable potential for mosquito control because these products are safer than conventional insecticides. This study aimed to investigate sublethal activities of Ipomoea carica or railway creeper crude acethonilic extract against life history trait of dengue vectors, Aedes albopictus and Aedes aegypti. The late third instar larvae of Ae. albopictus and Ae. aegypti were exposed to a sublethal dose at LC50 and larvae that survived were further cultured. Overall, Ipomea cairica crude extracts affected the whole life history of both Aedes species. The study demonstrated significantly lower egg production (fecundity) and eggs hatchability (fertility) in Ae. albopictus. The sublethal dose of crude extracts reduced significantly the width of larval head capsule and the wing length of both sexes in both Aedes species. The significance of sublethal effects of I. cairica against Aedes mosquitoes was an additional hallmark to demonstrate further activity of this plant despite its direct toxicity to the larvae. The reduced reproductive capacity as well as morphological and physiological anomalies are some of the effects that make I. cairica a potential candidate to be used as a new plant-based insecticide to control dengue vectors. PMID:27253746

  1. Phytochemical screening, antioxidant, and antimicrobial activities of the crude leaves′ extract from Ipomoea batatas (L. Lam

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    Márcia Thaís Pochapski

    2011-01-01

    Full Text Available Background: Ipomoea batatas (L. Lam., popularly known as sweet potato (SP, has played an important role as an energy and a phytochemical source in human nutrition and animal feeding. Ethnopharmacological data show that SP leaves have been effectively used in herbal medicine to treat inflammatory and/or infectious oral diseases in Brazil. The aim of this research was to evaluate the phytochemical, antioxidant, and antimicrobial activities of the crude leaves′ extract of SP leaves. Materials and Methods: The screening was performed for triterpenes/steroids, alkaloids, anthraquinones, coumarins, flavonoids, saponins, tannins, and phenolic acids. The color intensity or the precipitate formation was used as analytical responses to these tests. The total antioxidant capacity was evaluated by the phosphomolybdenum complex method. Antimicrobial activity was made by agar disk and agar well diffusion tests. Results: The phytochemical screening showed positive results for triterpenes/steroids, alkaloids, anthraquinones, coumarins, flavonoids, saponins, tannins, and phenolic acids. Total contents of 345.65, 328.44, and 662.02 mg were respectively obtained for alkaloids, anthraquinones, and phenolic compounds in 100 g of the dry sample. The total antioxidant capacity was 42.94% as compared to ascorbic acid. For antimicrobial studies, no concentration of the SP freeze dried extract was able to inhibit the growth of Streptococcus mutans, S. mitis, Staphylococcus aureus, and Candida albicans in both agar disk and agar well diffusion tests. Conclusions: SP leaves demonstrated the presence of secondary metabolites with potential biological activities. No antimicrobial activity was observed.

  2. Natural history of the narrow endemics Ipomoea cavalcantei and I. marabaensis from Amazon Canga savannahs.

    Science.gov (United States)

    Babiychuk, Elena; Kushnir, Sergei; Vasconcelos, Santelmo; Dias, Mariana Costa; Carvalho-Filho, Nelson; Nunes, Gisele Lopes; Dos Santos, Jorge Filipe; Tyski, Lourival; da Silva, Delmo Fonseca; Castilho, Alexandre; Fonseca, Vera Lucia Imperatriz; Oliveira, Guilherme

    2017-08-08

    Amazon comprises a vast variety of ecosystems, including savannah-like Canga barrens that evolved on iron-lateritic rock plateaus of the Carajás Mountain range. Individual Cangas are enclosed by the rain forest, indicating insular isolation that enables speciation and plant community differentiation. To establish a framework for the research on natural history and conservation management of endemic Canga species, seven chloroplast DNA loci and an ITS2 nuclear DNA locus were used to study natural molecular variation of the red flowered Ipomoea cavalcantei and the lilac flowered I. marabaensis. Partitioning of the nuclear and chloroplast gene alleles strongly suggested that the species share the most recent common ancestor, pointing a new independent event of the red flower origin in the genus. Chloroplast gene allele analysis showed strong genetic differentiation between Canga populations, implying a limited role of seed dispersal in exchange of individuals between Cangas. Closed haplotype network topology indicated a requirement for the paternal inheritance in generation of cytoplasmic genetic variation. Tenfold higher nucleotide diversity in the nuclear ITS2 sequences distinguished I. cavalcantei from I. marabaensis, implying a different pace of evolutionary changes. Thus, Canga ecosystems offer powerful venues for the study of speciation, multitrait adaptation and the origins of genetic variation.

  3. Development of Successive Cambia and Structure of Secondary Xylem of Ipomoea Obscura (Convolvulaceae

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    Rajput Kishore S.

    2014-07-01

    Full Text Available Stems of Ipomoea obscura Ker Gawl., increase in thickness by forming multiple rings of cambia. Stems 5-6 mm thick produce parenchymatous derivatives which divide repeatedly to form small arcs of cambium. Several such small arcs initiate simultaneously and form a ring of small cambial arcs. After the formation of a few xylem and phloem elements, all these arcs are interconnected by transdifferentiation of parenchyma cells present between the cambial arcs and constitute a complete cambial cylinder. This newly formed cambium is functionally bidirectional: earlier- formed arcs produce xylem centripetally and phloem centrifugally, while later-formed segments exclusively produce thin-walled parenchyma cells on either side. Young stems are circular in cross section but as stem thickness increases they become oval to elliptic or lobed and dumbbell-shaped. Xylem rays are mostly uni- or biseriate and thin-walled, but multiseriate rays characteristic for a climbing habit are observed occasionally. In thick stems, the marginal ray parenchyma in most of the samples becomes meristematic and develops ray cambia which exclusively produce sieve elements. Similarly, parenchyma cells produced from later-formed cambial segments give rise to several irregularly oriented vascular bundles. The secondary xylem is diffuse porous, with indistinct growth rings and is composed of fibriform and wider vessels, fibres, and axial and ray parenchyma cells, while phloem consists of sieve elements, companion cells, and axial and ray parenchyma cells.

  4. Hydrogen Sulfide Promotes Root Organogenesis in Ipomoea batatas, Salix matsudana and Glycine max

    Institute of Scientific and Technical Information of China (English)

    Hua Zhang; Jun Tang; Xiao-Ping Liu; Yun Wang; Wei Yu; Wei-Yan Peng; Fang Fang; Dai-Fu Ma; Zhao-Jun Wei; Lan-Ying Hu

    2009-01-01

    In this report, we demonstrate that sodium hydrosulfide (NaHS), a hydrogen sulfide (H2S) donor, promoted adventitious root formation mediated by auxin and nitric oxide (NO). Application of the H2S donor to seedling cuttings of sweet potato (Ipomoea batatas L.) promoted the number and length of adventltious roots in a dose-dependent manner. It was also verified that H2S or HS- rather than other sulfur-containing components derived from NariS could be attributed to the stimulation of adventitious root formation. A rapid Increase In endogenous H2S, indole acetic acid (IAA) and NO were sequentially observed in shoot tips of sweet potato seedlings treated with HallS. Further investigation showed that HzS-mediated root formation was alleviated by N-l-naphthylphthalamic acid (NPA), an IAA transport inhibitor, and 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO), an NO scavenger. Similar phenomena in H2S donor-dependent root organogenesis were observed in both excised willow (Sallx matsudana var. tortuosa Vilm) shoots and soybean (Glycine max L.) seedlings. These results indicated that the process of H2S-induced adventitious root formation was likely mediated by IAA and NO, and that H2S acts upstream of IAA and NO signal transduction pathways.

  5. Resin glycosides from Ipomoea wolcottiana as modulators of the multidrug resistance phenotype in vitro.

    Science.gov (United States)

    Corona-Castañeda, Berenice; Rosas-Ramírez, Daniel; Castañeda-Gómez, Jhon; Aparicio-Cuevas, Manuel Alejandro; Fragoso-Serrano, Mabel; Figueroa-González, Gabriela; Pereda-Miranda, Rogelio

    2016-03-01

    Recycling liquid chromatography was used for the isolation and purification of resin glycosides from the CHCl3-soluble extracts prepared using flowers of Ipomoea wolcottiana Rose var. wolcottiana. Bioassay-guided fractionation, using modulation of both antibiotic activity against multidrug-resistant strains of Gram-negative bacteria and vinblastine susceptibility in breast carcinoma cells, was used to isolate the active glycolipids as modulators of the multidrug resistance phenotype. An ester-type dimer, wolcottine I, one tetra- and three pentasaccharides, wolcottinosides I-IV, in addition to the known intrapilosin VII, were characterized by NMR spectroscopy and mass spectrometry. In vitro assays established that none of these metabolites displayed antibacterial activity (MIC>512 μg/mL) against multidrug-resistant strains of Escherichia coli, and two nosocomial pathogens: Salmonella enterica serovar Typhi and Shigella flexneri; however, when tested (25 μg/mL) in combination with tetracycline, kanamycin or chloramphenicol, they exerted a potentiation effect of the antibiotic susceptibility up to eightfold (64 μg/mL from 512 μg/mL). It was also determined that these non-cytotoxic (CI50>8.68 μM) agents modulated vinblastine susceptibility at 25 μg/mL in MFC-7/Vin(+) cells with a reversal factor (RFMCF-7/Vin(+)) of 2-130 fold.

  6. Resin glycosides from Ipomoea tyrianthina and their sedative and vasorelaxant effects.

    Science.gov (United States)

    León-Rivera, Ismael; Castro, José Manuel; Mirón-López, Gumersindo; del Río-Portilla, Federico; Enríquez, Raúl G; Reynolds, William F; Estrada-Soto, Samuel; Rendón-Vallejo, Priscilla; del Carmen Gutiérrez, María; Herrera-Ruiz, Maribel; Mendoza, Angeles; Vargas, Gabriela

    2014-10-01

    The methanol-soluble extract from the root of Ipomoea tyrianthina was studied in order to isolate compounds with activity on the central nervous system and vasorelaxant effects. Chromatographic methods were used to isolate and purify seven new glycolipids (2-8). The structures of compounds 1-8 were elucidated by a combination of NMR spectroscopy and mass spectrometry. Tyrianthinoic acid (1) is a glycosidic acid composed of a linear pentasaccharide core bonded to a 11-hydroxyhexadecanoic acid. The structure of tyrianthinic acids III (2), IV (3), and V (4) consists of a partially acylated tyrianthinoic acid. Tyrianthinic acid VI (8) is a tetrasaccharide core bonded to a jalapinolic acid, acylated by a 2-methyl-3-hydroxybutanoic acid. Tyrianthins C (5), D (6), and E (7) are ester-type heterodimers of scammonic acid A with different acylating residues in the two monomeric units. The macrolactonization site was located at C-3 of the rhamnose unit. The position of the ester linkage for monomeric unit B on the macrocyclic unit A was established at C-4 of the terminal quinovose. Compounds 5-7 increased the sleeping time induced by pentobarbital and the release of gamma-aminobutyric acid in brain cortex. In addition, compounds 5-7 showed significant in vitro relaxant effects on aortic rat rings, in endothelium- and concentration-dependent manners.

  7. Phytochemical screening, antioxidant, and antimicrobial activities of the crude leaves’ extract from Ipomoea batatas (L.) Lam

    Science.gov (United States)

    Pochapski, Márcia Thaís; Fosquiera, Eliana Cristina; Esmerino, Luís Antônio; dos Santos, Elizabete Brasil; Farago, Paulo Vitor; Santos, Fábio André; Groppo, Francisco Carlos

    2011-01-01

    Background: Ipomoea batatas (L.) Lam., popularly known as sweet potato (SP), has played an important role as an energy and a phytochemical source in human nutrition and animal feeding. Ethnopharmacological data show that SP leaves have been effectively used in herbal medicine to treat inflammatory and/or infectious oral diseases in Brazil. The aim of this research was to evaluate the phytochemical, antioxidant, and antimicrobial activities of the crude leaves’ extract of SP leaves. Materials and Methods: The screening was performed for triterpenes/steroids, alkaloids, anthraquinones, coumarins, flavonoids, saponins, tannins, and phenolic acids. The color intensity or the precipitate formation was used as analytical responses to these tests. The total antioxidant capacity was evaluated by the phosphomolybdenum complex method. Antimicrobial activity was made by agar disk and agar well diffusion tests. Results: The phytochemical screening showed positive results for triterpenes/steroids, alkaloids, anthraquinones, coumarins, flavonoids, saponins, tannins, and phenolic acids. Total contents of 345.65, 328.44, and 662.02 mg were respectively obtained for alkaloids, anthraquinones, and phenolic compounds in 100 g of the dry sample. The total antioxidant capacity was 42.94% as compared to ascorbic acid. For antimicrobial studies, no concentration of the SP freeze dried extract was able to inhibit the growth of Streptococcus mutans, S. mitis, Staphylococcus aureus, and Candida albicans in both agar disk and agar well diffusion tests. Conclusions: SP leaves demonstrated the presence of secondary metabolites with potential biological activities. No antimicrobial activity was observed. PMID:21716926

  8. Coronatine Induces an Accumulation of Anthocyanin and Starch in Purple-fleshed Sweetpotato (Ipomoea batatas Lam.)

    Institute of Scientific and Technical Information of China (English)

    Baoqing WANG; Beitao XIE; Haiyan ZHANG; Liming ZHANG; Qingmei WANG

    2013-01-01

    [Objective] The objective of this research was to examine the effects of COR on anthocyanin and starch content in storage roots of two PFS genotypes, and to explore the relationships between anthocyanin synthesis and starch accumula-tion. [Method] A field experiment was carried out to determine the changes in yield components, yield, contents of anthocyanin and starch, activities of phenylalanine ammonia-lyase (PAL) and adenosine 5-diphosphate glucose pyrophosphorylase (AG-Pase) in two genotypes of PFS (Ipomoea batatas L., var. ‘Ayamurasaki’ and‘Jishu18’). [Result] The application of COR significantly increased starch and antho-cyanin content in storage roots of Jishu18 across developmental stages by inducing the activities of PAL and AGPase, and final y enhanced yield by promoting fresh weight of storage roots. Ayamurasaki was insensitive to treatment with COR al-though its PAL activity temporal y increased. The starch and anthocyanin content of Aya, and the anthocyanin content of Jishu18 increased progressively across devel-opmental stages with or without COR application, but the starch content of Jishu18 increased initial y, then decreased before increasing again without application of COR. Treatment with COR reduced downward trend of starch accumulation in Jishu18. Thus, the effect of COR on accumulation of anthocyanin and starch in storage roots of PFS differs according to genotypes. [Conclusion] The application of 0.05 μmol/L COR may increase starch and anthocyanin content in PFS genotypes with lower starch and anthocyanin content in storage roots.

  9. Effect of aqueous extract of Ipomoea carnea leaf on isolated frog and mouse heart.

    Science.gov (United States)

    Bachhav, K V; Burande, M D; Rangari, V D; Mehta, J K

    1999-11-01

    Ipomoea carnea fam. Convolvulaceae is a poisonous plant and its toxicity is supposed to be due to the cardiac and respiratory failure. The present paper describes the cardiac effect of aqueous extract of the fresh leaves of I. carnea using mouse and frog heart. The aqueous extract produced an initial blockade of isolated frog heart for 5-10 sec followed by dose dependent increase in both amplitude and rate that lasts up to 2 min. Atropine (1 microgram/ml) blocked the initial depressant phase and potentiated the stimulant effect of the aqueous extract. The dose dependent increase in cardiac contractility of aqueous extract was not altered by propranolol or calcium channel blockers like nifedipine or diltiazem. The decrease in sodium chloride concentration or increase in potassium chloride concentration or calcium chloride concentration in physiological salt solution inhibited the responses to aqueous extract while an increase in sodium chloride concentration or decrease in potassium chloride or calcium chloride concentration in physiological salt solution potentiated the responses to the aqueous extract of I. carnea. It may be suggested from the data that aqueous extract of I. carnea produces positive inotropic effect on isolate frog heart possibly by sodium extrusion or release of the intracellular calcium.

  10. EVALUATION OF SUBLETHAL EFFECTS OF Ipomoea cairica LINN. EXTRACT ON LIFE HISTORY TRAITS OF DENGUE VECTORS

    Directory of Open Access Journals (Sweden)

    Wan Fatma ZUHARAH

    2016-01-01

    Full Text Available Plant derived insecticides have considerable potential for mosquito control because these products are safer than conventional insecticides. This study aimed to investigate sublethal activities of Ipomoea carica or railway creeper crude acethonilic extract against life history trait of dengue vectors, Aedes albopictus and Aedes aegypti. The late third instar larvae of Ae. albopictus and Ae. aegypti were exposed to a sublethal dose at LC50 and larvae that survived were further cultured. Overall, Ipomea cairica crude extracts affected the whole life history of both Aedes species. The study demonstrated significantly lower egg production (fecundity and eggs hatchability (fertility in Ae. albopictus. The sublethal dose of crude extracts reduced significantly the width of larval head capsule and the wing length of both sexes in both Aedes species. The significance of sublethal effects of I. cairica against Aedes mosquitoes was an additional hallmark to demonstrate further activity of this plant despite its direct toxicity to the larvae. The reduced reproductive capacity as well as morphological and physiological anomalies are some of the effects that make I. cairica a potential candidate to be used as a new plant-based insecticide to control dengue vectors.

  11. The role of alkaloids in Ipomoea carnea toxicosis: a study in rats.

    Science.gov (United States)

    Hueza, Isis M; Guerra, José L; Haraguchi, Mitsue; Naoki, Asano; Górniak, Silvana L

    2005-08-01

    Ipomoea carnea promotes in livestock a toxicosis histologically characterized by vacuolated cells in different organs. The toxic principles of I. carnea are the alkaloids swainsonine and calystegines B1, B2, B3 and Cl. However, it has not been determined whether the effects observed in rats treated with this plant are only due to swainsonine or if the calystegines have some additive toxic effect. Thus, the aim of the present study was to evaluate in rats the toxic effects of the L carnea aqueous fraction (AF) and of its different alkaloids when administered individually at the same concentration as in this fraction, for 14 days. No anorexic effect and/or alteration in body weight was observed in any group. The histopathologic study showed that while calystegines did not produce any toxic effects, swainsonine and I carnea AF promoted vacuolation in different organs, being more severe in the animals from the I. carnea AF group and extensible to other organs evaluated. No alterations were detected in the central nervous system of rats of any group assayed. The results obtained here suggest that calystegines may act as coadjuvants of swainsonine in I carnea toxicosis; however, little can be proposed about the neurotoxic effect of I. carnea since rats did not prove to be a good model for the reproduction of neuronal storage disease.

  12. Maternal Ingestion of Ipomoea carnea: Effects on Goat-Kid Bonding and Behavior

    Science.gov (United States)

    Gotardo, André T.; Pfister, James A.; Raspantini, Paulo C. F.; Górniak, Silvana L.

    2016-01-01

    Ipomoea carnea is a toxic plant found in Brazil and other tropical and subtropical countries and often causes poisoning of livestock. The plant contains the alkaloids swainsonine and calystegines, which inhibit key cellular enzymes and cause systematic cell death. This study evaluated the behavioral effects of prenatal ingestion of this plant on dams and their kids. Twenty-four pregnant goats were randomly allocated into four treatment groups and received the following doses (g/kg BW) of fresh I. carnea: 0 (control group), 1.0 (IC1), 3.0 (IC3), and 5.0 (IC5) from day 27 of gestation until parturition. Dam and kid bonding and behavior were evaluated by several tests, immediately after birth until six weeks of age. Dams from IC3 and IC5 groups spent less time paying attention to the newborn. There was a lack of maternal-infant bonding due to I. carnea intoxication. Kids from treated dams had difficulty in standing, suckling, and in recognizing their mother hours after birth. I. carnea can also compromise the kids’ ability to learn and to retain spatial memory. We suggest that kids from pregnant goats given I. carnea during gestation have significant behavioral alterations and developmental delays that may compromise their survival. PMID:26999204

  13. EVALUATION OF SUBLETHAL EFFECTS OF Ipomoea cairica LINN. EXTRACT ON LIFE HISTORY TRAITS OF DENGUE VECTORS.

    Science.gov (United States)

    Zuharah, Wan Fatma; Ahbirami, Rattanam; Dieng, Hamady; Thiagaletchumi, Maniam; Fadzly, Nik

    2016-01-01

    Plant derived insecticides have considerable potential for mosquito control because these products are safer than conventional insecticides. This study aimed to investigate sublethal activities of Ipomoea carica or railway creeper crude acethonilic extract against life history trait of dengue vectors, Aedes albopictus and Aedes aegypti. The late third instar larvae of Ae. albopictus and Ae. aegypti were exposed to a sublethal dose at LC50 and larvae that survived were further cultured. Overall, Ipomea cairica crude extracts affected the whole life history of both Aedes species. The study demonstrated significantly lower egg production (fecundity) and eggs hatchability (fertility) in Ae. albopictus. The sublethal dose of crude extracts reduced significantly the width of larval head capsule and the wing length of both sexes in both Aedes species. The significance of sublethal effects of I. cairica against Aedes mosquitoes was an additional hallmark to demonstrate further activity of this plant despite its direct toxicity to the larvae. The reduced reproductive capacity as well as morphological and physiological anomalies are some of the effects that make I. cairica a potential candidate to be used as a new plant-based insecticide to control dengue vectors.

  14. The immunomodulatory effects of Ipomoea carnea in rats vary depending on life stage.

    Science.gov (United States)

    Hueza, Isis M; Górniak, Silvana L

    2011-10-01

    Ipomoea carnea Jacq. ssp. fistulosa (Mart. Ex Choisy; Convolvulaceae; I. carnea) possesses a toxic component: an indolizidine alkaloid swainsonine (SW) that has immunomodulatory effects due to its inhibition of glycoprotein metabolism. It is also known that SW is excreted into both the amniotic fluid and milk of female rats exposed to I. carnea. Thus, the aim of this study was to determine whether SW exposure, either in utero or from the milk of dams treated with I. carnea, modulates offspring immune function into adulthood. In addition, adult (70 days old) and juvenile rats (21 days old) were exposed to I. carnea in order to evaluate several other immune parameters: lymphoid organs relative weight and cellularity, humoral and cellular immune responses. Offspring exposed to I. carnea during lactation developed rheumatoid arthritis (RA) in adulthood after an immunogenic challenge. In addition, both adult and juvenile rats exposed to I. carnea showed discrepancies in several immune parameters, but did not exhibit any decrease in humoral immune response, which was enhanced at both ages. These findings indicate that SW modulates immune function in adult rats exposed to SW during lactation and in juvenile and adult rats exposed to SW as juveniles and adults, respectively.

  15. Haematologic alterations caused by Ipomoea carnea in experimental poisoning of guinea pig.

    Science.gov (United States)

    García, Enrique N; Aguirre, María V; Gimeno, Eduardo J; Rios, Elvio E; Acosta, Ofelia C; Cholich, Luciana A

    2015-10-01

    Ipomoea carnea subsp. fistulosa (Convolvulaceae) causes poisoning of goats, sheep and cattle in many tropical and subtropical countries. The pathophysiology of this poisoning mainly involves an abnormal glycoprotein metabolism. The aim of this study was to describe the potential toxicity of I. carnea in a guinea pig model through its effect on hematopoiesis in a time course study of 40 days. Experimental poisoning was achieved by feeding animals with "small balls" prepared with milled leaves of I. carnea mixed with commercial crushed pellets for rodents. Hematologic and biochemical parameters, bone marrow and spleencellularities, histopathologic evaluations and lectin-histochemistrywere performed during the scheduled time of the study.The treatment with "small balls" caused significant changes in the weight of spleen, a notable decrease in peripheral red blood cells, and concomitantwith morphological and histopathologicalalterationsin hematopoietic tissues. Overall, the present study suggested that 20 days ofthis treatmentcouldbe enough to develop bone marrow hypoplasia and vacuolation of white cells of spleen, blood and lymph nodes with a transient erythropoietic contribution of the splenic niche.Moreover, this work provides a cheap and simple method for detecting preclinical cases of intoxication by I. carnea in livestock.

  16. Cryopreservation of sweetpotato (Ipomoea batatas) and its pathogen eradication by cryotherapy.

    Science.gov (United States)

    Feng, Chaohong; Yin, Zhenfang; Ma, Yanli; Zhang, Zhibo; Chen, Long; Wang, Biao; Li, Baiquan; Huang, Yushen; Wang, Qiaochun

    2011-01-01

    Sweetpotato (Ipomoea batatas) ranks as the seventh most important staple crop in the world and the fifth in developing countries after rice, wheat, maize and cassava. Sweetpotato is mainly grown in developing countries, which account for more than 95% of total production of the whole world. Genetic resources, including cultivated varieties and wild species, are a prerequisite for novel sweetpotato breeding in both conventional and genetic engineering programs. Various cryopreservation protocols have been developed for shoot tips and embryogenic tissues. The former explants are preferred for long-term conservation of sweetpotato genetic resources, while the latter are valuable for sweetpotato genetic improvement. This review provides update comprehensive information on cryopreservation of sweetpotato shoot tips and embryogenic tissues. Plant pathogens such as viruses and phytoplasma severely hamper high yield and high quality production of sweetpotato. Thus, usage of pathogen-free planting materials is pivotal for sustainable sweetpotato production. Cryotherapy of shoot tips can efficiently eradicate sweetpotato pathogens such as viruses and phytoplasma. The mechanism behind pathogen eradication by cryotherapy of shoot tips has been elucidated. Pathogen eradication by cryotherapy provides an alternative, efficient strategy for production of pathogen-free plants. In addition, cryopreserved tissues may also be considered to be safer for exchange of germplasm between countries and regions.

  17. Formulasi Krim Antioksidan Ekstrak Etanol Daun Ubi Jalar Ungu (Ipomoea batatas (L. Lamk Sebagai Anti Aging

    Directory of Open Access Journals (Sweden)

    Damaranie Dipahayu

    2014-12-01

    Full Text Available Purple sweet potatoes leaves (Ipomoea batatas (L. Lamk Antin 3 variety (IBLA are natural antioxidant sources because of its anthocyanins contents. IBLA was extracted with kinetic maseration method using 70% ethanolic solution. The aims of this research were determination of antioxidant activity of IBLA extract with DPPH (2,2-diphenyl-1-picrylhydrazil scavenging activity assay and formulation of IBLA extract into cream with oil in water (o/w base. Both the cream base and formulation were stored at 28oC for a period of 4 weeks to predict their stability. The evaluation parameters consisted of organoleptic, homogenity, pH value, viscosity, emulsion type, dispersive power, mechanic and freeze and thaw. Futhermore, the cream base and formulation were evaluated for their effects on skin moisture, curvature and pigmentation. The results showed that ethanolic extract of IBLA had antioxidant activity of 80.43 % compared to that of pure vitamin C. IC50 value of IBLA extract was 3.68 ppm while IC50 value of vitamin C was 2.96 ppm. The cream base and antioxidant cream had a physical stability for 4 weeks. The cream base and antioxidant cream had no effect on promoting skin moisture. The cream base had no effect in inhibiting curvature, but antioxidant cream was effective to inhibit curvature. The cream base showed no pigmentation inhibiting effect, but antioxidant cream was effective in inhibiting pigmentation.

  18. High Yield Culture Technique of Summer Ipomoea batatas in Binhai County%滨海县夏山芋高产栽培技术

    Institute of Scientific and Technical Information of China (English)

    李庆体; 金叶红; 迟金和; 李晴; 刘永

    2011-01-01

    从品质选择、育苗、地块选择、施肥、移栽、除草、病虫害防治、收获与贮藏等方面总结夏山芋高产栽培技术,以提高滨海县的山芋生产水平。%The high yield culture techniques of summer Ipomoea batatas were summarized from several aspects,such as variety selection,seeding,field selection,fertilization,transplantation,weeding,disease and insect control,harvesting and storage,so as to improve the production level of Ipomoea batatas in Binhai County.

  19. Identificación de virus en el cultivo de camote (Ipomoea batatas L. en Costa Rica

    Directory of Open Access Journals (Sweden)

    Rodrigo Valverde

    2004-01-01

    Full Text Available Identificación de virus en el cultivo de camote (Ipomoea batatas L. en Costa Rica. En el período entre febrero y mayo del 2002, se realizó un reconocimiento de los virus del cultivo de camote (Ipomoea batatas L., en cinco plantaciones comerciales ubicadas en las principales regiones productoras de este cultivo en Costa Rica. Previo al muestreo, en cada plantación se evaluó la incidencia de plantas con síntomas de enfermedades virales. Para la identificación de los virus, se recolectaron secciones apicales de tallo de plantas de camote con síntomas las cuales se injertaron sobre las plantas indicadoras de virus, de la especia Ipomoea setosa para inocularlas. Las muestras foliares de plantas inoculadas fueron analizadas serológicamente para el virus del moteado plumoso del camote (SPFMV y el virus del mosaico del pepino (CMV; con la reacción en cadena de polimerasa de transcripción reversa (RT-PCR para el virus del enanismo clorótico del camote (SPCSV y por hibridación molecular para el virus del enrollamiento de la hoja del camote (SPLCV. Las plantaciones ubicadas en el Cacao y el Coyol de Alajuela, y San Pedro de Santa Bárbara, presentaron las mayores incidencias de plantas con síntomas virales; a saber: 30, 90 y 90% respectivamente. El SPFMV, transmitido por los áfidos fue el virus más común. Otros virus detectados fueron el SPCSV y el SPLCV. El CMV, no se encontró en ninguna de las muestras analizadas.

  20. Wounding stimulates ALLENE OXIDE SYNTHASE gene and increases the level of jasmonic acid in Ipomoea nil cotyledons

    Directory of Open Access Journals (Sweden)

    Emilia Wilmowicz

    2016-03-01

    Full Text Available Allene oxide synthase (AOS encodes the first enzyme in the lipoxygenase pathway, which is responsible for jasmonic acid (JA formation. In this study we report the molecular cloning and characterization of InAOS from Ipomoea nil. The full-length gene is composed of 1662 bp and encodes for 519 amino acids. The predicted InAOS contains PLN02648 motif, which is evolutionarily conserved and characteristic for functional enzymatic proteins. We have shown that wounding led to a strong stimulation of the examined gene activity in cotyledons and an increase in JA level, which suggest that this compound may be a modulator of stress responses in I. nil.

  1. Evaluación de la dinámica del crecimiento in vitro en callos de Ipomoea batatas

    OpenAIRE

    Orlando S. González Paneque; Margarita Hernández Espinosa; Juan J Silva Pupo; Ángel Espinosa Reyes

    2011-01-01

    Evaluation of the dynamics of the growth in vitro callus of Ipomoea batatas ResumenEl cultivo del boniato presenta una gran importancia, ya que se puede emplear en la alimentación humana y animal, así como en la industria; el mismo produce raíces reservantes de gran valor calórico y nutritivo con alto contenido de carbohidratos. Entre las raíces y tubérculos cultivados es el segundo en importancia y representa más del 80% de la producción mundial. El empleo de las técnicas in vitro constituye...

  2. Integrated water quality, emergy and economic evaluation of three bioremediation treatment systems for eutrophic water

    Science.gov (United States)

    This study was targeted at finding one or more environmentally efficient, economically feasible and ecologically sustainable bioremediation treatment modes for eutrophic water. Three biological species, i.e. water spinach (Ipomoea aquatica), loach (Misgurus anguillicaudatus) and ...

  3. Intoxicação experimental por Ipomoea asarifolia (Convolvulaceae em caprinos e ovinos Experimental poisoning by Ipomoea asarifolia (Convolvulaceae in goats and sheep

    Directory of Open Access Journals (Sweden)

    José A.S. Araújo

    2008-10-01

    Full Text Available Ipomoea asarifolia causa uma síndrome tremorgênica em ovinos, caprinos, bovinos e búfalos. Este experimento teve como objetivos determinar a toxicidade para caprinos de I. asarifolia verde, colhidas nas épocas de chuva e de estiagem, e da planta seca triturada, determinar a toxicidade da planta para ovinos, e determinar se o princípio ativo da planta é eliminado pelo leite em doses tóxicas para os cordeiros. No primeiro experimento a planta fresca colhida na época de estiagem e na época de chuvas foi administrada a 16 caprinos. A planta colhida na estiagem foi tóxica na dose diária de 5 e 10g por kg de peso animal (g/kg. A planta colhida na época de chuva foi tóxica na dose diária de 20 e 30g/kg, demonstrando que a planta é mais tóxica durante o período seco. A planta seca, colhida na época de estiagem foi administrada a 9 caprinos em doses diárias de 1.7, 2, 3.4 e 5.1g/kg. Doses de 3, 4 e 5.1g/kg causaram sinais clínicos, demonstrando que a planta mantém a toxicidade após a secagem. A planta fresca colhida na época de estiagem e na época de chuvas foi administrada a 10 ovinos. A planta colhida na estiagem foi tóxica na dose diária de 5g/kg e na época de chuva foi tóxica nas doses de 10 e 20g/kg. Estes resultados sugerem a maior susceptibilidade dos ovinos à intoxicação do que os caprinos. Como alguns produtores mencionam que cordeiros lactentes que não estão pastando se intoxicam através do leite, I. asarifolia foi administrada diariamente nas doses de 2.5, 5 e 10g/kg a 5 ovelhas, a partir do dia do parto (2 ovelhas, do último dia de prenhez (1 ovelha e 60 dias antes da parição (2 ovelhas. As ovelhas, mas não os cordeiros, apresentaram sinais clínicos, sugerindo que o princípio ativo da planta não é eliminado no leite ou colostro em doses tóxicas para os cordeiros. Em um ovino eutanasiado não foram observadas lesões macroscópicas nem histológicas. Os achados ultra-estruturais mais significativos

  4. Quality improvement of sweet-potato (Ipomoea batatas L. Lam.) roots as feed by ensilage.

    Science.gov (United States)

    Lin, Y H; Huang, T C; Huang, C

    1988-07-01

    1. Sweet-potato (Ipomoea batatas L. Lam.) strips (SPS) mixed with maize powder (CP) in proportions 10:0, 9:1, 8:2, and 7:3 were ensiled for 1, 2 or 3 months. 2. Trypsin inhibitor activity (TIA) decreased during ensilage in samples of all treatments while the SPS-CP mixture (7:3, w/w) ensiled for 3 months contained the lowest TIA. 3. SPS-CP (8:2, w/w) dried or ensiled for 2 months, or ensiled for 2 months and dried, were each mixed with twice the amount of control diet (1:2, w/w) to make three diets. These three diets together with the control diet were used for a feeding experiment with rats to evaluate the nutritive value. 4. General composition analysis (including metabolizable energy), fatty acid composition and amino acid analysis (including percentage of essential amino acids) of the samples did not change during ensilage to an extent which could explain the improved performance of rats fed on ensiled diets. 5. Rats fed on diets containing dried SPS-CP (8:2, w/w) showed significantly lower (P less than 0.05) body-weight gain than rats fed on the control diet or ensiled SPS diets, at the end of the 8th week. They also showed enlargement of the pancreas. The adverse effect of SPS was associated with TIA which seemed to be prevented to some extent by ensilage. 6. The possibility that the starch of SPS may also contribute to the adverse effect cannot be excluded at present.

  5. In vitro micropropagation and flowering in Ipomoea sepiaria Roxb. An important ethanomedicinal plant

    Institute of Scientific and Technical Information of China (English)

    Meena K Cheruvathur; Jyothi Abraham; T Dennis Thomas

    2015-01-01

    Objective: To standardize a protocol for the micropropagation and in vitro flowering of Ipomoea sepiaria(I. sepiaria), an important ethanomedicinal plant. Methods: The nodal cuttings were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of 6-benzyladenine (BA) or Kinetin (Kn; 1.0-4.0 mg/L) alone or in combination withα-naphthaleneacetic acid (NAA; 0.2-1.0 mg/L) for shoot proliferation. For rooting ½ MS medium supplemented with indole-3-butyric acid (IBA) or NAA (0.5-3.0 mg/L) was used. When the 45-day-old in vitro derived nodal cuttings were subcultured on MS medium supplemented with 3.0 mg/L BA and 0.5 mg/L NAA and various concentrations of abscisic acid (ABA; 0.5-3.0 mg/L), in vitro flowering was observed. Results:The highest shoot induction response in terms of percent cultures responding and number of shoots per explant was observed on 3.0 mg/L BA and 0.5 mg/L NAA. On this medium 100%cultures responded with an average number of 3.2 shoots per explant. The optimum rooting was observed on 2.0 mg/L IBA. Here 100%shoots rooted with an average number of 5.1 roots per shoot. The optimum in vitro flowering response (38%) was observed on 2.0 mg/L ABA. Conclusion:The present protocol is an efficient method for the rapid multiplication, flowering and conservation of this medicinal plant.

  6. Identification of QTLs for Starch Content in Sweetpotato (Ipomoea batatas (L.) Lam.)

    Institute of Scientific and Technical Information of China (English)

    YU Xiao-xia; ZHAO Ning; LI Hui; JIE Qin; ZHAI Hong; HE Shao-zhen; LI Qiang; LIU Qing-chang

    2014-01-01

    Sweetpotato (Ipomoea batatas (L.) Lam.) breeding is challenging due to its genetic complexity. In the present study, interval mapping (IM) and multiple quantitative trait locus (QTL) model (MQM) analysis were used to identify QTLs for starch content with a mapping population consisting of 202 F1 individuals of a cross between Xushu 18, a cultivar susceptible to stem nematodes, with high yield and moderate starch, and Xu 781, which is resistant to stem nematodes, has low yield and high starch content. Six QTLs for starch content were mapped on six linkage groups of the Xu 781 map, explaining 9.1-38.8% of the variation. Especially, one of them,DMFN_4, accounted for 38.8% of starch content variation, which is the QTL that explains the highest phenotypic variation detected to date in sweetpotato. All of the six QTLs had a positive effect on the variation of the starch content, which indicated the inheritance derived from the parent Xu 781. Two QTLs for starch content were detected on two linkage groups of the Xushu 18 map, explaining 14.3 and 16.1% of the variation, respectively. They had a negative effect on the variation, indicating the inheritance derived from Xu 781. Seven of eight QTLs were co-localized with a single marker. This is the ifrst report on the development of QTLs co-localized with a single marker in sweetpotato. These QTLs and their co-localized markers may be used in marker-assisted breeding for the starch content of sweetpotato.

  7. Assessment of the perinatal effects of maternal ingestion of Ipomoea carnea in rats.

    Science.gov (United States)

    Hueza, Isis M; Guerra, José L; Haraguchi, Mitsue; Gardner, Dale R; Asano, Naoki; Ikeda, Kyoko; Górniak, Silvana L

    2007-08-01

    It is believed that Ipomoea carnea toxicosis induces abnormal embryogenesis in livestock. Studies on rats treated with I. carnea aqueous fraction (AF) during gestation, revealed litters with decreased body weight, but the characteristic vacuolar lesions promoted by swainsonine, its main toxic principle, were observed only in young rats on postnatal day (PND) 7. However, these alterations could have resulted as consequence of swainsonine placental passage and/or damage or even ingestion of the contaminated milk by pups. Thus, this perinatal work was performed to verify the transplacental passage of swainsonine and its excretion into milk employing the cross-fostering (CF) procedure as a tool of study. Females were treated with AF or vehicle during gestation and after birth pups were fostered between treated and untreated dams. Pup body weight gain (BWG) and histopathology to observe vacuolar degeneration were performed on PND 3 and 7. In addition, swainsonine detection was performed in amniotic fluid and milk from rats treated with the AF during gestation or lactation. BWG was significantly lower only in pups from mothers treated with the plant and fostered to other treated mothers (AF-AF group of pups). The histopathology revealed that pups from treated mothers fostered to untreated ones showed the characteristic vacuolar lesions; however, the lesions from the AF-AF pups were more severe in both periods evaluated. Amniotic fluid and milk analysis revealed the presence of swainsonine excretion into these fluid compartments. Thus, the results from CF and the chemical analysis allowed concluding that swainsonine passes the placental barrier and affects fetal development and milk excretion participates in I. carnea perinatal toxicosis.

  8. Experimental intoxication of guinea pigs with Ipomoea carnea: behavioural and neuropathological alterations.

    Science.gov (United States)

    Cholich, Luciana A; Márquez, Mercedes; Pumarola i Batlle, Martí; Gimeno, Eduardo J; Teibler, Gladys P; Rios, Elvio E; Acosta, Ofelia C

    2013-12-15

    Ipomoea carnea is a toxic plant that affects goats, with symptoms being characterised by nervous disorders and death. Swainsonine and calystegines are the principal toxic components isolated from I. carnea, which also yields lysergic acid derivatives. The aim of this study was to improve the clinical characterisation of experimental intoxication by I. carnea in guinea pigs through the evaluation of behavioural changes and to perform a thorough histopathological analysis of the affected CNS. Leaves of I. carnea were administered to guinea pigs. Open-field gait analysis and monoamine levels were measured. The poisoned animals exhibited increased vocalisation, lethargy, and a reduction in the locomotion frequency after the fourth week of intoxication, as demonstrated in the open-field test. Significant differences were observed in hind-limb gait width by the last week of intoxication. After 65 days, the guinea pigs were euthanised, necropsied, and examined using light and electron microscopy. At the end of the experiment, plasma serotonin decreased. In contrast, dopamine decreased, and noradrenaline increased in urine. Brain sections were evaluated with conventional histological methods and immunohistochemistry (IHC), as well as by transmission electron microscopy (TEM). Vacuoles were observed throughout the brain, but they were particularly prominent in the brainstem. In addition, there were PAS-negative regions, and the Nissl substance was dispersed or absent, which was confirmed with the Kluver-Barreda stain. Moderate microgliosis was observed by immunohistochemistry. In the medulla oblongata, numerous ubiquitin-positive spheroids together with neuronal degeneration were observed in the nucleus gracilis/cuneatus. Furthermore, vacuoles were observed in astrocytes, oligodendrocytes, and endothelial cells by TEM. Our results showed that the behavioural effects may have been caused by alterations in the brain in conjunction with changes in monoamine levels. This

  9. Towards a better understanding of Ipomoea asarifolia toxicity: evidence of the involvement of a leaf lectin.

    Science.gov (United States)

    Salles, H O; Vasconcelos, I M; Santos, L F L; Oliveira, H D; Costa, P P C; Nascimento, N R F; Santos, C F; Sousa, D F; Jorge, A R C; Menezes, D B; Monteiro, H S A; Gondim, D M F; Oliveira, J T A

    2011-11-01

    Natural intoxication of livestock by ingestion of Ipomoea asarifolia leaves has been reported to occur widely in Brazil. Previous studies carried out by our research group provided strong evidence that a lectin could be involved with the toxic properties of I. asarifolia. To reinforce this hypothesis, a lectin-enriched fraction (LEF) was isolated from I. asarifolia leaves and its toxic effects were assessed. Leaves of I. asarifolia were excised from plants growing widely in the field, mechanically wounded and maintained in a chamber at 25 ± 3 °C for 72h in the dark, under near 100% relative humidity. The leaf proteins were extracted, ammonium sulfate precipitated, chromatographed on DEAE-cellulose and Phenyl-Sepharose to produce LEF that under SDS-PAGE showed a molecular mass of 44.0 kDa and after N-terminal amino acid analysis a primary sequence composed of AGYTPVLDIGAEVLAAGEPY. The in vivo toxicity of LEF assessed by intraorbital injection in mice showed induced severe uncoordinated movements without death. LEF reduced the muscular contraction in a dose depend way and at 29.8 μg/mL (CE(50)) it produces 50% inhibition of contraction, suggesting that LEF blunts autonomic neurotransmission. Isolated rat kidneys were perfused with LEF and no effects on the perfusion pressure or renal vascular resistance were observed, but urinary flow and glomerular filtration rate increased. Moreover, the percentage of tubular transport of Na(+), K(+) and Cl(-) decreased. Histological examination of the kidneys perfused with LEF exhibited little alterations. These toxic effects observed above were concomitant with the increase of LEF hemagglutination activity, which strongly suggest that one of the toxic principles of I. asarifolia is a lectin present in its leaves.

  10. Effects of prepartum ingestion of Ipomoea carnea on postpartum maternal and neonate behavior in goats.

    Science.gov (United States)

    Gotardo, Andre T; Pfister, James A; Ferreira, Marcos Barbosa; Górniak, Silvana Lima

    2011-04-01

    Ipomoea carnea is a toxic plant that grows in tropical areas, and is readily consumed by grazing goats. The plant contains the alkaloids swainsonine and calystegines, which inhibit cellular enzymes and cause systematic cell death. This study evaluated the behavioral effects on dams and kids of prenatal ingestion of this plant. Freshly harvested leaves of I. carnea (10 g/kg body weight) were fed daily to nine pregnant goats from the fifth to the 16th week of gestation; five pregnant goats were controls. Dam and kid behavior were evaluated during 2-hr postpartum. Further evaluation of the offspring was performed using various tests after birth: (1) reaching and discriminating their dam from an alien doe (two tests at 12-hr postpartum), and (2) navigating a progressive maze (2, 4, and 6 days postpartum). Postnatal (n = 2) and fetal (n = 2) mortality were observed in the treated group. Intoxicated kids had difficulty in standing at birth, and only one was able to suckle within 2 hr of birth. Treated kids were slower than controls to arrive at their dam in the discrimination test; treated kids often (seven of nine completed tests) incorrectly chose the alien dam (controls: 0/10 tests). During some runs on days 2, 4, and 6 postpartum, treated kids were slower to leave the starting point of the maze, and were slower to arrive at the dam on all test days. This study suggests that the offspring of pregnant goats given I. carnea during gestation have significant behavioral alterations and developmental delays.

  11. Distributions, ex situ conservation priorities, and genetic resource potential of crop wild relatives of sweetpotato [Ipomoea batatas (L.) Lam., I. series Batatas

    NARCIS (Netherlands)

    Khoury, C.K.; Heider, B.; Castaneda-Alvarez, N.P.; Achicanoy, H.A.; Sosa, C.C.; Miller, R.E.; Scotland, R.W.; Wood, J.R.I.; Rossel, G.; Eserman, L.A.; Jarret, R.L.; Yencho, G.C.; Bernau, V.; Juarez, H.; Sotelo, S.; Haan, de S.; Struik, P.C.

    2015-01-01

    Crop wild relatives of sweetpotato [Ipomoea batatas (L.) Lam., I. series Batatas] have the potential to contribute to breeding objectives for this important root crop. Uncertainty in regard to species boundaries and their phylogenetic relationships, the limited availability of germplasm with which t

  12. Doença de depósito lisossomal induzida pelo consumo de Ipomoea verbascoidea (Convolvulaceae) em caprinos no semiárido de Pernambuco

    Science.gov (United States)

    The aim of this paper was to reproduce the poisoning of Ipomoea verbascoidea in goats and describe the epidemiological, clinical and pathological aspects of spontaneous poisoning by this plant in Pernambuco. For this, we studied the epidemiology of the disease in seven mu¬nicipalities in the semiari...

  13. Distributions, ex situ conservation priorities, and genetic resources potential of crop wild relatives of sweeetpotato (Ipomoea batatas (L.) Lam. I. series Batatas)

    Science.gov (United States)

    Crop wild relatives (CWR) of sweetpotato [Ipomoea batatas (L.) Lam., I. series Batatas] have the potential to contribute to breeding objectives for this important root crop. Uncertainty in regard to species boundaries and their phylogenetic relationships, and the limited availability of germplasm wi...

  14. Distributions, ex situ conservation priorities, and genetic resource potential of crop wild relatives of sweetpotato [Ipomoea batatas (L.) Lam., I. series Batatas

    NARCIS (Netherlands)

    Khoury, C.K.; Heider, B.; Castaneda-Alvarez, N.P.; Achicanoy, H.A.; Sosa, C.C.; Miller, R.E.; Scotland, R.W.; Wood, J.R.I.; Rossel, G.; Eserman, L.A.; Jarret, R.L.; Yencho, G.C.; Bernau, V.; Juarez, H.; Sotelo, S.; Haan, de S.; Struik, P.C.

    2015-01-01

    Crop wild relatives of sweetpotato [Ipomoea batatas (L.) Lam., I. series Batatas] have the potential to contribute to breeding objectives for this important root crop. Uncertainty in regard to species boundaries and their phylogenetic relationships, the limited availability of germplasm with which t

  15. Trypanocidal efficacy of two indigeneous ethanolic plant extracts (Mimosa pigra and Ipomoea asarifolia) against Trypanosoma evansi phospholipase A2 activity

    Institute of Scientific and Technical Information of China (English)

    Yusuf Alkali; A K Gana; Abdulkadir A; Nzelibe C Humphrey

    2015-01-01

    Objective:To study the inhibitory activity of ethanolic extract from Mimosa pigra and Ipomoea asarifolia against Trypanosoma evansi (T. evansi) calcium dependent phospholipase A2. Methods: The calcium dependent phospholipase A2 (E C 3.1.1.4) enzyme was isolated from T. evansi and purified to electrophoretic homogeneity under non denaturing conditions. It was solubilized from T. evansi cells recovered from white albino rats which were previously inoculated by intraperitoneal injection of infected camel blood. Two indigeneous ethanolic plant extracts used locally for treatment of trypanosomiasis were tested for the inhibition of phospholipases A2. Results: Double reciprocal plots of the initial velocity data of the inhibition by the indigenous plant extracts revealed a noncompetitive pattern of inhibition for the Ipomoea asarifolia and a competitive inhibition for Mimosa pigra in a dose dependent fashion. The extrapolated inhibition binding constant (Ki) of these extracts were found to be 2.0í102μg/mL and 1.12í102μg/mL respectively. Conclusions:The low Ki values obtained for these extracts towards this enzyme are an indication of high affinity of the extract or the active components (present in the plants) are for these enzyme and therefore, could be explored to serve as a cheap source of T. evansi PLA2 antidote and as well help in designing a novel drug with high efficiency.

  16. Natural association of two different betasatellites with Sweet potato leaf curl virus in wild morning glory (Ipomoea purpurea) in India.

    Science.gov (United States)

    Swapna Geetanjali, A; Shilpi, S; Mandal, Bikash

    2013-08-01

    Wild morning glory (Ipomoea purpurea) was observed to be affected by leaf curl and yellow vein diseases during summer-rainy season of 2009 in New Delhi, India. The virus was experimentally transmitted through whitefly, Bemisia tabaci to I. purpurea that reproduced the two distinct symptoms. Sequence analysis of multiple full-length clones obtained through rolling circle amplification from the leaf curl and yellow vein samples showed 91.8-95.3% sequence identity with Sweet potato leaf curl virus (SPLCV) and the isolates were phylogenetically distinct from those reported from Brazil, China, Japan and USA. Interestingly, two different betasatellites, croton yellow vein mosaic betasatellite and papaya leaf curl betasatellite were found with SPLCV in leaf curl and yellow vein diseases of I. purpurea, respectively. This study is the first report of occurrence of SPLCV in wild morning glory in India. SPLCV was known to infect other species of morning glory; our study revealed that I. purpurea, a new species of morning glory was a natural host of SPLCV. To date, betasatellite associated with SPLCV in Ipomoea spp. is not known. Our study provides evidence of natural association of two different betasatellites with SPLCV in leaf curl and yellow vein diseases of I. purpurea.

  17. Development of successive cambia, cambial activity, and their relationship to physiological traits in Ipomoea arborescens (Convolvulaceae) seedlings.

    Science.gov (United States)

    Terrazas, Teresa; Aguilar-Rodríguez, Silvia; Ojanguren, Clara Tinoco

    2011-05-01

    The seedling stage is one of the most critical phases in the life history of plants; during this stage, plants must develop efficient conductive and storage systems before the end of the favorable season. Little is known about the origin of successive cambia in seedlings of tree species of Ipomoea or about how many cambia are produced in one growth season. We studied the anatomy of Ipomoea arborescens seedlings to defi ne when cambium is differentiated, to determine how many cambia differentiate in one year of growth, and to relate the development of successive cambia to physiological aspects of growth. Seedlings from 5 to 425 d of age were harvested, and their morphology as well as CO(2) and water exchange, were evaluated at 5 and 60 d after germination. Six stages of development were established to study origin of cambia. Cambium was differentiated 5 d after germination, at a time when seedlings had photosynthetic cotyledons with high specific area, assimilation rate, and stomatal conductance. Differentiation of the fi rst successive cambium occurred inparenchyma cells below the endodermis or starch sheath. Development of reverse cambium and intraxylary phloem cambiumdemonstrated that ontogenetic shifts may occur in different stem regions. In the 10-mo-old plants, all cambia reactivated, and earlywood wide vessels were differentiated. The origin of successive cambia, the occurrence of more than one type of cambium, and parenchyma proliferation are features shared by I. arborescens with its climbing ancestors as a strategy for survival in the harsh environment of tropical deciduous forests.

  18. Chemical Constituents from the Tubers of Ipomoea batata%番薯块根化学成分研究

    Institute of Scientific and Technical Information of China (English)

    尹永芹; 沈志滨; 孔令义

    2012-01-01

    目的:研究番薯块根的化学成分.方法:95%乙醇提取回收溶剂后以氯仿萃取,萃取部位经多种层析方法分离;结合理化性质和波谱方法进行结构鉴定.结果:分离并鉴定了6个化合物,分别为:Batatinoside Ⅰ(1)、枸橼苦素(2)、咖啡酸十八烷酯(3)、乙酰-β-香树醇(4)、咖啡酸(5)、东莨菪素(6).结论:其中,化合物1为结构新颖的树脂糖苷类化合物,并首次从番薯中分离得到.%Objective: To investigate the chemical constituents from the tubers of Ipomoea batata. Methods: The chemical constituents were isolated and purified by solvent extraction together with various chromatographic techniques. The structures were elucidated on the basis of physiochemical property and spectral data. Results: 6 compounds were identified from the CHC13 extract as Batatinoside 1(1), citrusin C(2), octadecyl caffeate (3) , β-amyrin acetate (4) , caffeic acid (5) ,scopoletin (6). Conclusion; Compound 1 is I-solated from Ipomoea batata for the first time.

  19. De novo assembly and annotation of the transcriptome of the agricultural weed Ipomoea purpurea uncovers gene expression changes associated with herbicide resistance.

    Science.gov (United States)

    Leslie, Trent; Baucom, Regina S

    2014-08-25

    Human-mediated selection can lead to rapid evolution in very short time scales, and the evolution of herbicide resistance in agricultural weeds is an excellent example of this phenomenon. The common morning glory, Ipomoea purpurea, is resistant to the herbicide glyphosate, but genetic investigations of this trait have been hampered by the lack of genomic resources for this species. Here, we present the annotated transcriptome of the common morning glory, Ipomoea purpurea, along with an examination of whole genome expression profiling to assess potential gene expression differences between three artificially selected herbicide resistant lines and three susceptible lines. The assembled Ipomoea transcriptome reported in this work contains 65,459 assembled transcripts, ~28,000 of which were functionally annotated by assignment to Gene Ontology categories. Our RNA-seq survey using this reference transcriptome identified 19 differentially expressed genes associated with resistance-one of which, a cytochrome P450, belongs to a large plant family of genes involved in xenobiotic detoxification. The differentially expressed genes also broadly implicated receptor-like kinases, which were down-regulated in the resistant lines, and other growth and defense genes, which were up-regulated in resistant lines. Interestingly, the target of glyphosate-EPSP synthase-was not overexpressed in the resistant Ipomoea lines as in other glyphosate resistant weeds. Overall, this work identifies potential candidate resistance loci for future investigations and dramatically increases genomic resources for this species. The assembled transcriptome presented herein will also provide a valuable resource to the Ipomoea community, as well as to those interested in utilizing the close relationship between the Convolvulaceae and the Solanaceae for phylogenetic and comparative genomics examinations.

  20. Rats offspring exposed to Ipomoea Carnea and handling during gestation: neurochemical evaluation

    Directory of Open Access Journals (Sweden)

    Aline Schwarz

    2007-05-01

    Full Text Available The present study evaluated the central monoamine levels of male and female adult rat offspring exposed orally by gavage to 0.0, 0.7, 3.0 and 15.0 mg/kg I. carnea aqueous extract daily, from gestation day (GD 5 to GD 21. Several alterations in the monoamine activity systems were observed. However, the major differences were noted between the 0.0 mg/kg and the no gavage control groups, showing that alterations showing that alterations were not due to the alterations to the aqueous extract. The control data showed that gavage and handling of dams were stressful enough to produce a significant decline in 3,4-dihydroxyphenylacetic acid (DOPAC and an increase in vanilmandelic acid (VMA, indicating decreased dopamine (DA and enhanced norepinephrine (NE activity, respectively.Estudo anterior realizado em filhotes de ratas tratadas diariamente por gavage com 0,0, 0,7, 3,0 e 15,0 mg/kg de uma solução aquosa obtida de folhas frescas da Ipomoea carnea, do dia 5 ao dia 21 da gestação, mostrou poucas alterações comportamentais na prole em vida adulta. O presente estudo teve como objetivo avaliar a atividade e níveis das monoaminas cerebrais nas proles masculina e feminina expostas ao mesmo tratamento acima descrito. As maiores alterações encontradas, entretanto, foram entre os grupos 0,0 mg/kg e controle negativo (no gavage, impedindo a atribuição das alterações encontradas à solução aquosa. O dados resultantes do grupo controle sugerem que o estresse provocado pela gavage e pelo manuseio das fêmeas enquanto prenhes é suficiente para produzir um importante declínio nos níveis do ácido 3,4 dihidroxifenilacético (DOPAC e um não menos importante aumento nos níveis do ácido vanilmandélico (VMA, promovendo maior atividade do sistema noradrenérgico (NE.

  1. Anti-inflammatory and antispasmodic activity of Ipomoea imperati (Vahl Griseb (Convolvulaceae

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    A.C.B. Paula

    2003-01-01

    Full Text Available Ipomoea imperati (Convolvulaceae lives on the sandy shores of the Brazilian coast and in other areas of the world. The anti-inflammatory activity of a methanol-water extract of the leaves of I. imperati was investigated in experimental models of acute and subchronic inflammation. Topical application of the extract (10 mg/ear inhibited mouse ear edema induced by croton oil (89.0 ± 1.3% by the lipid fraction with an IC50 of 3.97 mg/ear and 57.0 ± 1.3% by the aqueous fraction with an IC50 of 3.5 mg/ear and arachidonic acid (42.0 ± 2.0% with an IC50 of 4.98 mg/ear and 31.0 ± 2.0% with an IC50 of 4.72 mg/ear. Phospholipase A2, purified from Apis mellifera bee venom, was also inhibited by the extract (5.0 mg/ml lipid and aqueous fraction in vitro in a dose-dependent manner (85% by the lipid fraction with an IC50 of 3.22 mg/ml and 25% by the aqueous fraction with an IC50 of 3.43 mg/ml. The methanol-water extract of I. imperati (1000 mg/kg administered by the oral route also inhibited the formation of cotton pellet-induced granulomas (73.2 ± 1.2% by the lipid fraction and 56.14 ± 2.7% by the aqueous fraction and did not cause gastric mucosal lesions. I. imperati extracts (10 mg/ml also inhibited in a dose-dependent manner the muscle contractions of guinea pig ileum induced by acetylcholine and histamine (IC50 of 1.60 mg/ml for the lipid fraction and 4.12 mg/ml for the aqueous fraction. These results suggest the use of I. imperati as an anti-inflammatory and antispasmodic agent in traditional medicine.

  2. Listeria floridensis sp. nov., Listeria aquatica sp. nov., Listeria cornellensis sp. nov., Listeria riparia sp. nov. and Listeria grandensis sp. nov., from agricultural and natural environments.

    Science.gov (United States)

    den Bakker, Henk C; Warchocki, Steven; Wright, Emily M; Allred, Adam F; Ahlstrom, Christina; Manuel, Clyde S; Stasiewicz, Matthew J; Burrell, Angela; Roof, Sherry; Strawn, Laura K; Fortes, Esther; Nightingale, Kendra K; Kephart, Daniel; Wiedmann, Martin

    2014-06-01

    Sampling of agricultural and natural environments in two US states (Colorado and Florida) yielded 18 Listeria-like isolates that could not be assigned to previously described species using traditional methods. Using whole-genome sequencing and traditional phenotypic methods, we identified five novel species, each with a genome-wide average BLAST nucleotide identity (ANIb) of less than 85% to currently described species. Phylogenetic analysis based on 16S rRNA gene sequences and amino acid sequences of 31 conserved loci showed the existence of four well-supported clades within the genus Listeria; (i) a clade representing Listeria monocytogenes, L. marthii, L. innocua, L. welshimeri, L. seeligeri and L. ivanovii, which we refer to as Listeria sensu stricto, (ii) a clade consisting of Listeria fleischmannii and two newly described species, Listeria aquatica sp. nov. (type strain FSL S10-1188(T) = DSM 26686(T) = LMG 28120(T) = BEI NR-42633(T)) and Listeria floridensis sp. nov. (type strain FSL S10-1187(T) = DSM 26687(T) = LMG 28121(T) = BEI NR-42632(T)), (iii) a clade consisting of Listeria rocourtiae, L. weihenstephanensis and three novel species, Listeria cornellensis sp. nov. (type strain TTU A1-0210(T) = FSL F6-0969(T) = DSM 26689(T) = LMG 28123(T) = BEI NR-42630(T)), Listeria grandensis sp. nov. (type strain TTU A1-0212(T) = FSL F6-0971(T) = DSM 26688(T) = LMG 28122(T) = BEI NR-42631(T)) and Listeria riparia sp. nov. (type strain FSL S10-1204(T) = DSM 26685(T) = LMG 28119(T) = BEI NR- 42634(T)) and (iv) a clade containing Listeria grayi. Genomic and phenotypic data suggest that the novel species are non-pathogenic.

  3. Purification and Trypsin Inhibitor Activity of a Sporamin B from Sweet Potato (Ipomoea batatas Lam. 55-2)

    Institute of Scientific and Technical Information of China (English)

    SUN Yan-li; SUN Jun-mao; LI Qing-peng

    2009-01-01

    Sporamin is a soluble protein in sweet potato, and falls into two distinct homology groups, subfamilies A and B. In this research, a sporamin B was purified and its amino acid sequences, trypsin inhibitor activity (Ti activity) were analyzed. This sporamin B was isolated from sweet potato tubers [Ipomoea batatas (L.) Lam cv. 55-2] through extraction of the water-soluble fraction, dialysis, ultrafiltration and ion-exchange chromatography. Homology determined by polyacrylamide gel electrophoresis showed that mainly one bond appeared in gel after being reduced by SDS (sodium dodecyl sulfate), or by SDS and 2-mercaptoethanol, or in native situation. By comparing the data of the polypeptide mass Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry with those of the mass of the theoretical amino acid sequences from NCBI protein database, it was revealed that it was Q40091|Q40091_IPOBA, sweet potato sporamin B - Ipomoea Batatas (sweet potato) (Batate). The sequence coverage was 70.6%. N-terminal sequence was SETPV (Ser-Glu-Thr-Pro-Val). There is a linear relationship between trypsin inhibitor activity (Ti activity) and amounts of this sporamin B (3-18 μg mL-1). The equation of linear regression was y=2.5809x+17.049 (r2=0.9966). There was a curvilinear relationship between Ti activity and amounts of this sporamin B (21-150 μg mL-1). The equation of curvilinear regression is y= 14.4171n(x)+23.26 (r2=0.9924). The concentration of sporamin B with Ti activity after heating at 40℃ may induce part denature of this sporamin B, and there was no statistic difference after heating at 40, 50, 60℃ for 20 min. Heat treatment at more than 90℃ leads to a dramatic decrease of trypsin inhibitor efficiency. The results suggested that Q40091|Q40091_IPOBA was the major sporamin B in sweet potato tubers [Ipomoea Batatas (L.) Lain cv. 55-2], which had strong Ti activity, and was stable to both thermal and DTT (DL-dithiothreitol) relatively.

  4. PEMANFAATAN UBI JALAR UNGU (Ipomoea batatas L. Poir SEBAGAI PENGGANTI SEBAGIAN TEPUNG TERIGU DAN SUMBER ANTIOKSIDAN PADA ROTI TAWAR [Purple Sweet Potato (Ipomoea batatas L. Poir as a Partial Subtitute of Wheat flour and Source of Antioxidant on Plain Bread

    Directory of Open Access Journals (Sweden)

    Hardoko*

    2010-06-01

    Full Text Available Purple sweet potato (Ipomoea batatas L. Poir is potential for functional food, especially as antioxidant source due to its purple color and other nutrient content. The objective of this research was to produce plain bread containing antioxidant by partial substitution of wheat flour with purple sweet potato flour. The result showed that the best plain bread was produced by a maximum substitution of 20% purple sweet potato flour to wheat flour. The hedonic characteristics of the resulting bread including aroma, taste, and texture were not significantly different from bread without substitution. Nevertheless, the crust was harder and darker. Addition of 1.0% GMS emulsifier and reduction of purple potato flour to 15% increased the score of softness, hedonic texture, acceptance level, as well as the volume of the bread. The substituted bread had antioxidant activity of 55833.78 ppm DPPH as shown by the IC50 value and contained 4.30% of dietary fiber.

  5. Superação da dormência de sementes de corda-de-viola (Ipomoea quamoclit e I. hederifolia Overcoming dormancy of Ipomoea quamoclit and I. hederifolia seeds

    Directory of Open Access Journals (Sweden)

    C.A.M. Azania

    2009-03-01

    Full Text Available Este estudo teve como objetivo testar o uso da água como método de superação de dormência de sementes de Ipomoea hederifolia e I. quamoclit (cordas-de-viola. Antes da realização dos tratamentos, as sementes das duas espécies foram submetidas ao teste de tetrazólio, para avaliar a sua viabilidade. Os tratamentos utilizados constituíram-se do uso de água quente em ponto de ebulição (98 ºC e em temperatura ambiente (26 ºC, submetendo-se as sementes de ambas as espécies a diferentes períodos de exposição (zero; 0,5; 1; 5; 10; 15; 20; e 24 horas. A emergência das plântulas foi determinada diariamente, durante 21 dias, para posterior cálculo da porcentagem e da velocidade de emergência. As espécies de Ipomoea tiveram comportamento diferenciado. As sementes de I. hederifolia submetidas ao tratamento com água quente apresentaram maior porcentagem de emergência de plântulas que a da testemunha, a partir de 0,5 hora de embebição; os tratamentos mantidos em água à temperatura ambiente não foram eficientes para superar a dormência desta espécie. Para sementes de I. quamoclit, os tratamentos estudados não tiveram efeito na superação da dormência, sendo prejudiciais à sua qualidade.An experiment was installed in Ribeirao Preto-SP to test the use of water as a method of overcoming dormancy of Ipomoea hederifolia and I. quamoclit seeds. Before carrying out the treatments, the seeds of the two species were submitted to tetrazolium test to assess their viability. The treatments consisted in submerging the seeds of both species in hot water at boiling point (98 ºC and in water at ambient temperature (26 ºC, during different periods of exposure (null; 0,5; 1; 5; 10; 15; 20 and 24 hours. Seedling emergence was determined daily, for 21 days, for posterior calculation of emergence speed rate and emergence speed. The Ipomoea species presented a differentiated behavior. The I. hederifolia seeds subjected to the hot water

  6. In vivo anti-fatigue activity of total flavonoids from sweetpotato [Ipomoea batatas (L.) Lam.] leaf in mice.

    Science.gov (United States)

    Li, Chunguang; Zhang, Lianying

    2013-08-01

    The in vivo anti-fatigue activity of the total flavonoids from sweet potato [Ipomoea batatas (L.) Lam.] leaf was investigated in male Kunming mice. The total flavonoids from sweet potato leaf (TFSL) were orally administered at doses of 50, 100 and 200 mg/kg for 4 weeks and the anti-fatigue effect was studied using a weight-loaded swimming test, along with the determination of serum urea nitrogen (SUN), blood lactic acid (BLA) and hepatic and muscle glycogen contents. The results showed that TFSL had significant anti-fatigue effects. TFSL extended the exhaustive swimming time, effectively inhibited the increase of BLA, decreased the level of SUN and increased the hepatic and muscle glycogen content of mice. Thus, TFSL may have potential as an anti-fatigue agent.

  7. Climatic factors influence leaf structure and thereby affect the ozone sensitivity of Ipomoea nil 'Scarlet O'Hara'.

    Science.gov (United States)

    Moura, Bárbara B; Alves, Edenise S

    2014-11-01

    Phenotypic plasticity of the leaves can interfere with the plant sensitivity to ozone (O3) toxic effect. This study aimed to assess whether the leaf structure of Ipomoea nil changes due to climatic variations and whether these changes affect the species' sensitivity. Field exposures, in different seasons (winter and spring) were made. The leaves that developed during the winter were thinner, with a lower proportion of photosynthetic tissues, higher proportion of intercellular spaces and lower density and stomatal index compared to those developed during the spring. The temperature and relative humidity positively influenced the leaf thickness and stomatal index. The visible injuries during winter were positively correlated with the palisade parenchyma thickness and negatively correlated with the percentage of spongy parenchyma; during the spring, the symptoms were positively correlated with the stomatal density. In conclusion, the leaf structure of I. nil varied among the seasons, interfering in its sensitivity to O3.

  8. Expressed nifH Genes of Endophytic Bacteria Detected in Field-Grown Sweet Potatoes (Ipomoea batatas L.).

    Science.gov (United States)

    Terakado-Tonooka, Junko; Ohwaki, Yoshinari; Yamakawa, Hiromoto; Tanaka, Fukuyo; Yoneyama, Tadakatsu; Fujihara, Shinsuke

    2008-01-01

    We examined the nitrogenase reductase (nifH) genes of endophytic diazotrophic bacteria expressed in field-grown sweet potatoes (Ipomoea batatas L.) by reverse transcription (RT)-PCR. Gene fragments corresponding to nifH were amplified from mRNA obtained from the stems and storage roots of field-grown sweet potatoes several months after planting. Sequence analysis revealed that these clones were homologous to the nifH sequences of Bradyrhizobium, Pelomonas, and Bacillus sp. in the DNA database. Investigation of the nifH genes amplified from the genomic DNA extracted from these sweet potatoes also showed high similarity to various α-proteobacteria including Bradyrhizobium, β-proteobacteria, and cyanobacteria. These results suggest that bradyrhizobia colonize and express nifH genes not only in the root nodules of leguminous plants but also in sweet potatoes as diazotrophic endophytes.

  9. Elaboración de sopa instantánea a partir de harina de ipomoea batatas(camote)

    OpenAIRE

    Albán, Carlos; Figueroa, Astrid; Cornejo, Fabiola

    2011-01-01

    Se elaboró una sopa instantánea a partir de la harina de camote, ya que además de ser rica en nutrientes se quiere es rescatar el consumo de productos autóctonos. Primero se realizó la caracterización de la materia prima (Ipomoea batatas), mediante ensayos físico-químico, después se elaboró la isoterma del camote, las curvas de secado y la caracterización de la harina de camote. Después de la obtención de la harina se realizó diferentes formulaciones con el objetivo de determinar la más adecu...

  10. Analysis of Metabolites in Stem Parasitic Plant Interactions: Interaction of Cuscuta–Momordica versus Cassytha–Ipomoea

    Directory of Open Access Journals (Sweden)

    Takeshi Furuhashi

    2016-12-01

    Full Text Available Cuscuta and Cassytha are two well-known stem parasitic plant genera with reduced leaves and roots, inducing haustoria in their stems. Their similar appearance in the field has been recognized, but few comparative studies on their respective plant interactions are available. To compare their interactions, we conducted a metabolite analysis of both the Cassytha–Ipomoea and the Cuscuta–Momordica interaction. We investigated the energy charge of the metabolites by UFLC (ultra-high performance liquid chromatography, and conducted GC-MS (gas chromatography-mass spectrometry analysis for polar metabolites (e.g., saccharides, polyols and steroids. The energy charge after parasitization changed considerably in Cassytha but not in Cusucta. Cuscuta changed its steroid pattern during the plant interaction, whereas Cassytha did not. In the polar metabolite analysis, the laminaribiose increase after parasitization was conspicuous in Cuscuta, but not in Cassytha. This metabolite profile difference points to different lifestyles and parasitic strategies.

  11. Analysis of Metabolites in Stem Parasitic Plant Interactions: Interaction of Cuscuta–Momordica versus Cassytha–Ipomoea

    Science.gov (United States)

    Furuhashi, Takeshi; Nakamura, Takemichi; Iwase, Koji

    2016-01-01

    Cuscuta and Cassytha are two well-known stem parasitic plant genera with reduced leaves and roots, inducing haustoria in their stems. Their similar appearance in the field has been recognized, but few comparative studies on their respective plant interactions are available. To compare their interactions, we conducted a metabolite analysis of both the Cassytha–Ipomoea and the Cuscuta–Momordica interaction. We investigated the energy charge of the metabolites by UFLC (ultra-high performance liquid chromatography), and conducted GC-MS (gas chromatography-mass spectrometry) analysis for polar metabolites (e.g., saccharides, polyols) and steroids. The energy charge after parasitization changed considerably in Cassytha but not in Cusucta. Cuscuta changed its steroid pattern during the plant interaction, whereas Cassytha did not. In the polar metabolite analysis, the laminaribiose increase after parasitization was conspicuous in Cuscuta, but not in Cassytha. This metabolite profile difference points to different lifestyles and parasitic strategies. PMID:27941603

  12. Poisoning by Ipomoea asarifolia in lambs by the ingestion of milk from ewes that ingest the plant.

    Science.gov (United States)

    Carvalho de Lucena, Kleber F; Rodrigues, Jussara M N; Campos, Édipo M; Dantas, Antônio F M; Pfister, James A; Cook, Daniel; Medeiros, Rosane M T; Riet-Correa, Franklin

    2014-12-15

    Two experiments, each with 10 pregnant ewes (8 treated and 2 controls) were performed to determine if nursing lambs of lactating ewes become intoxicated when the ewes ingest Ipomoea asarifolia but do not show clinical signs themselves. In the first experiment the sheep grazed I. asarifolia in the field while in the second, sheep were maintained in individual bays consuming dry I. asarifolia at 10% and 20% into their ration. In both experiments the lambs remained confined, consuming only their mother's milk. Four of 8 lambs in the grazing experiment and the 4 nursing lambs from the ewes given 20% I. asarifolia showed signs of I. asarifolia poisoning. These results confirm that the tremorgenic compound of I. asarifolia or its toxic metabolites are eliminated in milk and can intoxicate nursing lambs.

  13. High tolerance to salinity and herbivory stresses may explain the expansion of Ipomoea cairica to salt marshes.

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    Gang Liu

    Full Text Available BACKGROUND: Invasive plants are often confronted with heterogeneous environments and various stress factors during their secondary phase of invasion into more stressful habitats. A high tolerance to stress factors may allow exotics to successfully invade stressful environments. Ipomoea cairica, a vigorous invader in South China, has recently been expanding into salt marshes. METHODOLOGY/PRINCIPAL FINDINGS: To examine why this liana species is able to invade a stressful saline environment, we utilized I. cairica and 3 non-invasive species for a greenhouse experiment. The plants were subjected to three levels of salinity (i.e., watered with 0, 4 and 8 g L(-1 NaCl solutions and simulated herbivory (0, 25 and 50% of the leaf area excised treatments. The relative growth rate (RGR of I. cairica was significantly higher than the RGR of non-invasive species under both stress treatments. The growth performance of I. cairica was not significantly affected by either stress factor, while that of the non-invasive species was significantly inhibited. The leaf condensed tannin content was generally lower in I. cairica than in the non-invasive I. triloba and Paederia foetida. Ipomoea cairica exhibited a relatively low resistance to herbivory, however, its tolerance to stress factors was significantly higher than either of the non-invasive species. CONCLUSIONS/SIGNIFICANCE: This is the first study examining the expansion of I. cairica to salt marshes in its introduced range. Our results suggest that the high tolerance of I. cairica to key stress factors (e.g., salinity and herbivory contributes to its invasion into salt marshes. For I. cairica, a trade-off in resource reallocation may allow increased resources to be allocated to tolerance and growth. This may contribute to a secondary invasion into stressful habitats. Finally, we suggest that I. cairica could spread further and successfully occupy salt marshes, and countermeasures based on herbivory could be

  14. Effect of municipal sludge and chemical fertilizers on phthalic acid esters (PAEs) contents in Ipomoea aquatica grown on paddy soils%城市污泥和化肥对水稻土种植的通菜中邻苯二甲酸酯(PAEs)的影响

    Institute of Scientific and Technical Information of China (English)

    蔡全英; 莫测辉; 朱夕珍; 吴启堂; 王伯光; 蒋成爱; 李海芹

    2003-01-01

    对水稻土施用城市污泥和化肥盆栽通菜,应用GC/MS联机检测技术对通菜中6种邻苯二甲酸酯化合物(PAEs)进行分析,探讨施肥对通菜中PAEs含量的影响.结果表明,各处理通菜中6种PAEs化合物的总含量(∑PAEs)在2.129~7.111mg*kg-1之间,依次为广州污泥+化肥(7.111mg*kg-1) >广州污泥(4.767 mg*kg-1)>佛山污泥(3.569mg*kg-1)>佛山污泥+化肥(3.305mg*kg-1)>化肥(2.638mg*kg-1)>空白对照(2.129mg*kg-1),显示了施肥造成通菜中∑PAEs不同程度的提高.各处理通菜中的PAEs均以个别化合物为主,其中空白对照、化肥、佛山污泥和广州污泥处理的通菜中邻苯二甲酸丁基苄基酯(BBP)占∑PAEs的 40%~81%,佛山污泥+化肥处理的通菜中邻苯二甲酸正二丁酯(DnBP)占∑PAEs的56%,而广州污泥+化肥处理的通菜中邻苯二甲酸丁基苄基酯(BBP)、邻苯二甲酸正二辛酯(DnOP)和邻苯二甲酸(2-乙基己基)酯(DEHP)各占∑PAEs的30%左右.通菜中∑PAEs和多数化合物的含量不同程度地大于其在根系中的含量.

  15. Obtención de Maltodextrinas por vía enzimática a partir del almidón de camote (Ipomoea batatas).

    OpenAIRE

    Medina García, Luis

    2013-01-01

    El objetivo del presente trabajo de investigación consistió en producir maltodextrinas por vía enzimática a partir del almidón del camote (Ipomoea batatas); materia prima que es ampliamente cultivada en varios estados de la República Mexicana. 5 Inicialmente se realizó el análisis químico proximal de los tubérculos del camote (Ipomoea batatas variedad nylon), después se llevó a cabo la extracción y caracterización del almidón y posteriormente se determinaron las condiciones de hidrólisi...

  16. Sweepoviruses cause disease in sweet potato and related Ipomoea spp.: fulfilling Koch's postulates for a divergent group in the genus begomovirus.

    Directory of Open Access Journals (Sweden)

    Helena P Trenado

    Full Text Available Sweet potato (Ipomoea batatas and related Ipomoea species are frequently infected by monopartite begomoviruses (genus Begomovirus, family Geminiviridae, known as sweepoviruses. Unlike other geminiviruses, the genomes of sweepoviruses have been recalcitrant to rendering infectious clones to date. Thus, Koch's postulates have not been fullfilled for any of the viruses in this group. Three novel species of sweepoviruses have recently been described in Spain: Sweet potato leaf curl Lanzarote virus (SPLCLaV, Sweet potato leaf curl Spain virus (SPLCSV and Sweet potato leaf curl Canary virus (SPLCCaV. Here we describe the generation of the first infectious clone of an isolate (ES:MAL:BG30:06 of SPLCLaV. The clone consisted of a complete tandem dimeric viral genome in a binary vector. Successful infection by agroinoculation of several species of Ipomoea (including sweet potato and Nicotiana benthamiana was confirmed by PCR, dot blot and Southern blot hybridization. Symptoms observed in infected plants consisted of leaf curl, yellowing, growth reduction and vein yellowing. Two varieties of sweet potato, 'Beauregard' and 'Promesa', were infected by agroinoculation, and symptoms of leaf curl and interveinal loss of purple colouration were observed, respectively. The virus present in agroinfected plants was readily transmitted by the whitefly Bemisia tabaci to I. setosa plants. The progeny virus population present in agroinfected I. setosa and sweet potato plants was isolated and identity to the original isolate was confirmed by sequencing. Therefore, Koch's postulates were fulfilled for the first time for a sweepovirus.

  17. Intoxicação espontânea por Ipomoea carnea subsp. fistulosa (Convolvulaceae em bovinos no Pantanal Matogrossense Spontaneous Ipomoea carnea subsp. fistulosa (Convolvulaceae poisoning of cattle in the Brazilian Pantanal

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    Nadia A.B. Antoniassi

    2007-10-01

    Full Text Available Relata-se a intoxicação espontânea por Ipomoea carnea subsp. fistulosa (canudo, algodoeiro em bovinos no Pantanal Matogrossense. As investigações iniciaram após a morte de 12 bovinos, de um rebanho de 500 animais, criados em uma extensa área intensamente infestada por I. carnea subsp. fistulosa com escassa disponibilidade de outra forragem. As mortes ocorreram entres os meses de junho e setembro de 2006. O quadro clínico foi caracterizado por emagrecimento e sinais neurológicos com dificuldade locomotora. Um bovino foi necropsiado sem que se observassem alterações macroscópicas significativas. Histologicamente havia tumefação e vacuolização celular, em neurônios, células acinares pancreáticas, tubulares renais e foliculares da tireóide. Bovinos com quadro clínico similar foram retirados da área invadida por I. carnea subsp. fistulosa e colocadas em áreas com pastagem nativa e de Brachiaria sp. e apresentaram melhora clínica após período de 15 dias.A spontaneous Ipomoea carnea subsp. fistulosa (canudo, algodoeiro poisoning of cattle in the county of Poconé, Brazilian Pantanal, is reported. The investigation began after 12 cattle had died from a flock of 500 animals maintained in an extensive area intensely infested by I. carnea subsp. fistulosa with scarce availability of other fodder plants. The deaths occurred from June to September of 2006. Clinical signs were loss of weight and neurological deficits with hypermetry and incoordination. No significant gross lesions were observed at postmortem examination of one bovine. Histological changes comprised widespread cytoplasmic vacuolation of neurons, cells of the thyroid, kidney and pancreas. Cattle with similar clinical picture, that had been removed from the area invaded by I. carnea subsp. fistulosa and placed into areas with native and Brachiaria sp. pasture, recovered clinically within 15 days.

  18. Seed germination of Ipomoea pes-caprae (L.) Sweet%厚藤种子萌发特性

    Institute of Scientific and Technical Information of China (English)

    刘建强; 胡军飞; 欧丹燕; 胡冬冬; 金水虎

    2011-01-01

    以厚藤Ipomoea pes-caprae种子为试验材料,测定其种子的大小、千粒质量、硬实率、生活力以及在不同处理条件下的发芽势和发芽率.结果表明:机械处理下的效果最好,其种子平均发芽势和发芽率均提高到95.3%;其次为980 g·kg-1硫酸处理90min较好,平均发芽势达到91.2%,发芽率达93.5%;氢氧化钠处理也可明显提高厚藤种子萌发能力,400g·kg-1氢氧化钠处理36 h的平均发芽率可达79.4%;不同温度处理以25~30℃的萌发能力最强,其发芽势为24.6%,发芽率为43.4%.实际工作中,可以采用机械处理或酸碱处理的方法以提高厚藤种子的萌发能力.图2表4参18%In this research the influence of different treatments on seed germination characteristics of Ipomoea pes-caprae were studied to determine effective measures for enhancing the germination rate and germination potential. Treatments included a mechanical treatment, 98% H2SO4 solution for 90 min, 40% NaOH solution for 36 h, and a temperature treatment between 15 - 20, 20 - 25 and 25 - 30 ℃. Results showed that the mechanical treatment was best with both the average germination potential and average germination rate of 95.3%. Also, with the H2O4 solution, the average germination potential was 91.2%, and the average germination rate was 93.5%. The NaOH solution treatment improved the average germination potential to 79.4% and the average germination rate to 40%. For the temperature treatment, germination was highest when temperatures were between 25 - 30 ℃ with the average germination potential reaching 24.6% and the average germination rate attaining 43.4%. Thus, the mechanical, the 98% H2SO4, or the NaOH solution treatments could improve seed germination of I. pes-caprae.

  19. The degradation of two fluoroquinolone based antimicrobials by SilA, an alkaline laccase from Streptomyces ipomoeae.

    Science.gov (United States)

    Blánquez, Alba; Guillén, Francisco; Rodríguez, Juana; Arias, M Enriqueta; Hernández, Manuel

    2016-03-01

    The presence of fluoroquinolone based antimicrobials in natural waters represents a significant emerging environmental problem. In this study the suitability of a novel alkaline bacterial laccase, SilA, from Streptomyces ipomoeae to degrade two key antimicrobials, Ciprofloxacin and Norfloxacin under alkaline conditions in the presence of natural mediators was assessed. Results showed that only the selected SilA-acetosyringone system was able to degrade more than 90% of both fluoroquinolones. HPLC analysis of the degradation products obtained after enzyme treatment confirmed the disappearance of the antimicrobials and the mediator after 24 h. The time course of the degradation showed that during the first 4 h a 75% of degradation of fluoroquinolones was detected while the mediator remained stable. A concomitant appearance of new chromatographic peaks derived from the fluoroquinolones and/or the mediator was detected. Moreover, toxicity assays demonstrated that the SilA-acetosyringone system was able to reduce the toxicity of Ciprofloxacin and Norfloxacin by 90 and 70%, respectively. In conclusion, these findings support the suitability of a low cost and environmentally friendly strategy based on the SilA-acetosyringone system for a primary treatment of contaminated alkaline wastewaters with this type of emerging pollutants.

  20. Study on Pb Content in 3 Week and 6 Week Old Kangkung (Ipomoea reptans Poir Planted in Pb containing Media

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    Poppy Hartatie Hardjo

    2005-11-01

    Full Text Available A study on the content of Pb in kangkung has been conducted. Land kangkung (Ipomoea reptans was used as the sample, and was planted in hydrophonic media, and watered with Multigrow Complete Plant Food (2000 mg/L and Pb solution (2 mg/L twice a day. Samples were taken based on the age (3 and 6 week old, and part of the plant (root and all parts without root. Inductively Coupled Plasma Spectrometer (ICPS Fison 3410+ was used to measure the Pb content. It was shown that in the plant the accumulation was mostly happened in the root. The 6 week-old plant contained Pb not just in the root (3.36 mg/kg sample but also in the other part of the plant (2.09 mg/kg sample and those were exceeded the maximum dietary allowance (2 mg/kg sample regulated by the Indonesian FDA; while in the 3 week-old plant the Pb content in the root was 1.86 mg/kg sample and in the other part of the plan was 1.13 mg/kg, which is not exceeded the dietary allowance. So it is advisable to harvest the kangkung vegetable at the most of 3 week-old.

  1. Relationship between Processing Method and the Glycemic Indices of Ten Sweet Potato (Ipomoea batatas Cultivars Commonly Consumed in Jamaica

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    Perceval S. Bahado-Singh

    2011-01-01

    Full Text Available This study investigated the effect of different traditional cooking methods on glycemic index (GI and glycemic response of ten Sweet potato (Ipomoea batatas cultivars commonly eaten in Jamaica. Matured tubers were cooked by roasting, baking, frying, or boiling then immediately consumed by the ten nondiabetic test subjects (5 males and 5 females; mean age of 27 ± 2 years. The GI varied between 41 ± 5–93 ± 5 for the tubers studied. Samples prepared by boiling had the lowest GI (41 ± 5–50 ± 3, while those processed by baking (82 ± 3–94 ± 3 and roasting (79 ± 4–93 ± 2 had the highest GI values. The study indicates that the glycemic index of Jamaican sweet potatoes varies significantly with the method of preparation and to a lesser extent on intravarietal differences. Consumption of boiled sweet potatoes could minimize postprandial blood glucose spikes and therefore, may prove to be more efficacious in the management of type 2 diabetes mellitus.

  2. Atividade alelopática dos lixiviados de Asemeia extraaxillaris (Polygalaceae sobre o crescimento de Ipomoea cordifolia

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    C.B. SILVA

    2016-01-01

    Full Text Available RESUMO O objetivo desse trabalho foi avaliar a atividade alelopática dos lixiviados das raízes e folhas secas de Asemeia extraaxillaris sobre o crescimento de corda de viola Ipomoea cordifolia em casa de vegetação, para avaliação dos efeitos sobre o estresse oxidativo. Nos bioensaios foram utilizadas três concentrações (0,25 - 5,00 e 10,00 g e um controle, com quatro repetições de 10 plantas, cultivadas em solo durante 30 dias. Os lixiviados possuem ação alelopática e o maior efeito foi verificado para o pó das raizes. O efeito inibitório foi dose-dependente e o aumento do estresse oxidativo foi observado pelos níveis da catalase, peroxidase, superóxido dismutase, glutationa redutase, peroxidação lipídica e síntese de formazan. Concluiu-se que A. extraaxillaris possui substâncias capazes de afetar a emergência, o crescimento e o estresse oxidativo de I. cordifolia.

  3. A resurrection experiment finds evidence of both reduced genetic diversity and potential adaptive evolution in the agricultural weed Ipomoea purpurea.

    Science.gov (United States)

    Kuester, Adam; Wilson, Ariana; Chang, Shu-Mei; Baucom, Regina S

    2016-09-01

    Despite the negative economic and ecological impact of weeds, relatively little is known about the evolutionary mechanisms that influence their persistence in agricultural fields. Here, we use a resurrection approach to examine the potential for genotypic and phenotypic evolution in Ipomoea purpurea, an agricultural weed that is resistant to glyphosate, the most widely used herbicide in current-day agriculture. We found striking reductions in allelic diversity between cohorts sampled nine years apart (2003 vs. 2012), suggesting that populations of this species sampled from agricultural fields have experienced genetic bottleneck events that have led to lower neutral genetic diversity. Heterozygosity excess tests indicate that these bottlenecks may have occurred prior to 2003. A greenhouse assay of individuals sampled from the field as seed found that populations of this species, on average, exhibited modest increases in herbicide resistance over time. However, populations differed significantly between sampling years for resistance: some populations maintained high resistance between the sampling years whereas others exhibited increased or decreased resistance. Our results show that populations of this noxious weed, capable of adapting to strong selection imparted by herbicide application, may lose genetic variation as a result of this or other environmental factors. We probably uncovered only modest increases in resistance on average between sampling cohorts due to a strong and previously identified fitness cost of resistance in this species, along with the potential that nonresistant migrants germinate from the seed bank.

  4. Intoxicação por Ipomoea asarifolia em bovinos e ovinos no Rio Grande do Norte

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    Fabricio K. de L. Carvalho

    2014-11-01

    Full Text Available Ipomoea asarifolia é uma das principais plantas tóxicas para ruminantes nas regiões Norte e Nordeste. A intoxicação ocorre na época das secas quando há escassez de forragem e é mais frequente em ovinos do que em outras espécies, porem casos espontâneos também são relatados em caprinos, bovinos e búfalos. Este trabalho relata a ocorrência de 18 surtos de intoxicação em bovinos por I. asarifolia que ocorreram de agosto de 2012 a fevereiro de 2013 no estado do Rio Grande Norte. De um total de 1704 bovinos, 202 (11,8% adoeceram e 34 (2% morreram. Em seis propriedades onde havia ovinos estes também se intoxicaram. Foram observados também sinais clínicos em bezerros e cordeiros confinados, sem acesso às pastagens, que se intoxicaram pela ingestão do leite das mães que ingeriam I. asarifolia a pasto.

  5. Immunostimulatory acivity of Calophyllum brasiliense, Ipomoea pes-caprae and Matayba elaeagnoides demonstrated by human peripheral blood mononuclear cells proliferation.

    Science.gov (United States)

    Philippi, Marina Elisa; Duarte, Bruna Momm; Da Silva, Carolina Vieira; De Souza, Michel Thomaz; Niero, Rivaldo; Cechinel Filho, Valdir; Bueno, Edneia Casagranda

    2010-01-01

    This study evaluates the effect of methanol extracts of three Brazilian medicinal plants on in vitro proliferation of human mononuclear cells. Lymphoproliferation assay was carried out by incubating human peripheral blood mononuclear cells from healthy donors (1 x 10(6) cells/mL) with extracts of Calophyllum brasiliense (roots), Ipomoea pes-caprae (whole plant) and Matayba elaeagnoides (bark), both at 10, 50, 100 and 200 microg/mL, alone or with phytohemagglutinin (PHA, 5 microg/mL), in 96-well microplates at 37 degrees C with 5% CO2, for 72 h. The quantification of cell proliferation assay was performed by blue tetrazolium (MTT) reduction with reading at 540 nm. Cells incubated with only the culture medium were used as negative control for cell proliferation, while the positive control consisted of cells and PHA. The results suggest that the extracts of all three studied plants induce T lymphocyte proliferation. I. pes-caprae showed immunostimulatory activity three times higher than the C. brasiliense extract, while that of the M. elaeagnoides extract was 1.5 times higher. The results demonstrate immunostimulatory effects of these three plants, therefore the continuity of these studies is recommended, in order to determine the active principles.

  6. Chemical constituents of essential oils of Tephrosia purpurea and Ipomoea carnea and their repellent activity against Odoiporus longicollis

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    Sahayaraj Kitherian

    2015-01-01

    Full Text Available Chemical constituents of essential oils (EOs obtained from stem and root of Tephrosia purpurea (Linn. Pers. and Ipomoea carnea Jacq. were investigated by Gas Chromatography-Mass Spectrometry (GC-MS. Total lipid and oil content was high in the stem than the root of T. purpurea and I. carnea. Essential oils extracted from the stem and root of T. purpurea and I. carnea showed 9 and 8 compounds respectively. Hexadecanoic acid was found to be the principal constituent of stem (69.61% and root (46.97% of T. purpurea while 70.61and 88.89% for stem and root, respectively in the case of I. carnea. The findings of the present study suggest that T. purpurea and I. carnea EOs can be used as a source of hexadecanoic acid which could be used for industrial purposes. The essential oils of T. purpurea and I. carnea showed strong repellent activity for males (-0.73 and -0.70 for T. purpurea and I. carnea stem EO respectively than females (-0.63 and -0.59 for T. purpurea and I. carnea stem EO respectively against banana pseudostem weevil Odoiporus longicollis. The results indicated that the active compounds of essential oils from stems of T. purpurea and I. carnea can be explored as natural repellents for control of Odoiporus longicollis.

  7. Assessment of residual bio-efficacy and persistence of Ipomoea cairica plant extract against Culex quinquefasciatus Say mosquito.

    Science.gov (United States)

    Thiagaletchumi, Maniam; Zuharah, Wan Fatma; Ahbi Rami, Rattanam; Fadzly, Nik; Dieng, Hamady; Ahmad, Abu Hassan; AbuBakar, Sazaly

    2014-09-01

    Specification on residual action of a possible alternative insecticide derived from plant materials is important to determine minimum interval time between applications and the environmental persistence of the biopesticides. The objective of this study is to evaluate crude acethonilic extract of Ipomoea cairica leaves for its residual and persistence effects against Culex quinquefasciatus larvae. Wild strain of Cx. quinquefasciatus larvae were used for the purpose of the study. Two test designs, replenishment of water and without replenishment of water were carried out. For the first design, a total of 10 ml of test solution containing Ip. cairica extracts was replenished daily and replaced with 10 ml of distilled water. For the second design, treatment water was maintained at 1500 ml and only evaporated water was refilled. Larval mortality was recorded at 24 hours post-treatment after each introduction period and trials were terminated when mortality rate falls below 50%. Adult emergences from survived larvae were observed and number of survivals was recorded. For the non-replenishment design, mortality rate significantly reduced to below 50% after 28 days, meanwhile for replenishment of water declined significantly after 21 days (P water design. The short period of residual effectiveness of crude acethonilic extract of Ip. cairica leaves with high percentage of larval mortality on the first few days, endorses fewer concerns of having excess residues in the environment which may carry the risk of insecticide resistance and environmental pollution.

  8. Development of hydroethanolic extract of Ipomoea pes-caprae using factorial design followed by antinociceptive and anti-inflammatory evaluation

    Directory of Open Access Journals (Sweden)

    Daniela Vieira

    2012-01-01

    Full Text Available Ipomoea pes-caprae (L. R. Br., Convolvulaceae, is a medicinal plant that grows abundantly as a pan-tropical stand plant. The 3² (two factors and three levels factorial design, was applied to determine the best time and drug/solvent proportion to maximize the flavonoid content in the hydroethanolic extract by maceration process. The antinociceptive and anti-inflammatory effects were studied at 5-20 mg/kg, i.p., using the writhing test and carrageenan-induced pleurisy models in mice. The optimized extract was able to inhibit more than 50% of abdominal writhing at 20 mg/kg, with 55.88%±2.4 of maximum inhibition. Indomethacin, used as positive control, inhibited 64.86% at 10 mg/kg. In the pleurisy model, the extract produced dose-dependent inhibition of the first phase of inflammation (4 h in the pleural cavity induced by injection of carrageenan (1% in mice. It inhibited 50%±0.82 (p<0.01 of exudation induced by carrageenan, and 60.88%±0.14 (p<0.01 of leukocyte migration to the pleural cavity. In conclusion, the results validate the technological conditions of the maceration process to produce an optimized bioactive herb extract for the development of analgesic and anti-inflammatory phytopharmaceuticals using 70 ºGL ethanol, a plant to solvent ratio of 12.5% (w/v, and ten days of maceration.

  9. Cloning and Functional Analysis of Lycopeneε-Cyclase (IbLCYe) Gene from Sweetpotato, Ipomoea batatas (L.) Lam

    Institute of Scientific and Technical Information of China (English)

    YU Ling; ZHAI Hong; CHEN Wei; HE Shao-zhen; LIU Qing-chang

    2013-01-01

    This paper reported firstly successful cloning of lycopeneε-cyclase (IbLCYe) gene from sweetpotato, Ipomoea batatas (L.) Lam. Using rapid amplification of cDNA ends (RACE), IbLCYe gene was cloned from sweetpotato cv. Nongdafu 14 with high carotenoid content. The 1 805 bp cDNA sequence of IbLCYe gene contained a 1 236 bp open reading frame (ORF) encoding a 411 amino acids polypeptide with a molecular weight of 47 kDa and an isoelectric point (pI) of 6.95. IbLCYe protein contained one potential lycopeneε-cyclase domain and one potential FAD (flavinadenine dinucleotide)/NAD(P) (nicotinamide adenine dinucleotide phosphate)-binding domain, indicating that this protein shares the typical characteristics of LCYe proteins. The gDNA of IbLCYe gene was 4 029 bp and deduced to contain 5 introns and 6 exons. Real-time quantitative PCR analysis revealed that the expression level of IbLCYe gene was significantly higher in the storage roots of Nongdafu 14 than those in the leaves and stems. Transgenic tobacco (cv. Wisconsin 38) expressing IbLCYe gene accumulated significantly moreβ-carotene compared to the untransformed control plants. These results showed that IbLCYe gene has an important function for the accumulation of carotenoids of sweetpotato.

  10. Ipomoea obscura ameliorates cyclophosphamide-induced toxicity by modulating the immune system and levels of proinflammatory cytokine and GSH.

    Science.gov (United States)

    Hamsa, T P; Kuttan, Girija

    2010-11-01

    Ipomoea obscura L. is a widely used medicinal plant. The objective of this study was to investigate its protective activity against cyclophosphamide (CTX)-induced toxicity in mouse models. Swiss albino mice were treated intraperitoneally with CTX (25 mg/kg body weight) along with I. obscura extract (10 mg/kg body weight) for 10 days. Extract significantly reduced myelosuppression caused by CTX and improved the relative organ weight, total white blood cell count, and bone marrow cellularity. The elevated levels of parameters related to pathophysiology of the liver, namely glutamate pyruvate transaminase, alkaline phosphatase, and lipid peroxidation, were significantly reduced by extract treatment. Reduction of liver and intestinal glutathione levels of CTX-treated animals was reversed by I. obscura. The lowered levels of cytokines, namely IFN-γ, IL-2, and granulocyte-monocyte colony-stimulating factor after CTX treatment were found to be increased in I. obscura treated animals. Treatment with I. obscura could also decrease the level of proinflammatory cytokine TNF-α. The data suggested that I. obscura can act as a potent chemoprotective agent and can be used as an adjuvant in chemotherapeutic applications.

  11. Evaluation of the effect of an organic extract obtained from Ipomoea alba L. on experimental periodontitis in rats

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    Guilherme Emerson Barrella

    2012-04-01

    Full Text Available The aim of this study was to evaluate the effect of an organic extract obtained from Ipomoea alba L. (Convolvulaceae or OE 1493, on experimental periodontal disease in rats. Periodontitis was induced in thirty six Wistar rats: a first mandibular molar was randomly assigned to receive a ligature, whereas the contralateral molar was left unligated. Animals were randomly assigned to two groups and treated topically, three times a day, for 11 days, as follows: Control Group - vehicle-treated (n = 18, and Test Group - OE 1493-treated (n = 18. The rats were sacrificed on the 12th day. Morphometrical measurements from the cementoenamel junction to the bone crest were performed to determine alveolar bone loss, using standardized photographs. Single- and multi-dose acute toxicity assays were carried out after OE 1493 treatment. Morphometrical analysis demonstrated that topically-administered OE 1493 showed no effect on reducing bone loss when compared with the control group (p > 0.05. In addition, OE 1493 did not present toxicity. Within the limits of this investigation, it may be concluded that OE 1493 did not show any positive influence on the progression of ligature-induced periodontitis in rats, when administered according to the regimen used in the present study.

  12. Uptake and transformation of phenol and chlorophenols by hairy root cultures of Daucus carota, Ipomoea batatas and Solanum aviculare.

    Science.gov (United States)

    de Araujo, Brancilene Santos; Dec, Jerzy; Bollag, Jean Marc; Pletsch, Marcia

    2006-04-01

    Hairy root cultures of Daucus carota L., Ipomoea batatas L. and Solanum aviculare Forst were investigated for their susceptibility to the highly toxic pollutants phenol and chlorophenols and for the involvement of inherent peroxidases in the removal of phenols from liquid media. Roots of D. carota grew normally in medium containing 1000 micromol l(-1) of phenol, whilst normal growth of roots of I. batatas and S. aviculare was only possible at levels up to 500 micromol l(-1). In the presence of chlorophenols, normal root growth was possible only in concentrations not exceeding 50 micromol l(-1), except for I. batatas which was severely affected at all concentrations. Despite the reduction in biomass, the growth of S. aviculare cultures was sustained in medium containing up to 2000 micromol l(-1) of phenol or 2-chlorophenol, and up to 500 micromol l(-1) of 2,6-dichlorophenol. The amounts of phenol removed by the roots within 72 h of treatment were 72.7%, 90.7% and 98.6% of the initial concentration for D. carota, I. batatas and S. aviculare, respectively. For the removal of 2,6-dichlorophenol the values were, respectively, 83.0%, 57.7% and 73.1%. Phenols labelled with 14C were absorbed by the root tissues and condensed with highly polar cellular substances as well as being incorporated into the cell walls or membranes. The results suggest that S. aviculare, an ornamental plant, would be best suited for remediation trials under field conditions.

  13. Induction of systemic resistance in rice by leaf extracts of Zizyphus jujuba and Ipomoea carnea against Rhizoctonia solani.

    Science.gov (United States)

    Kagale, Sateesh; Marimuthu, Thambiayya; Kagale, Jayashree; Thayumanavan, Balsamy; Samiyappan, Ramasamy

    2011-07-01

    Plants accumulate a great diversity of natural products, many of which confer protective effects against phytopathogenic attack. Earlier we had demonstrated that the leaf extracts of Zizyphus jujuba and Ipomoea carnea inhibit the in vitro mycelial growth of Rhizoctonia solani, and effectively reduce the incidence of sheath blight disease in rice. Here we demonstrate that foliar application of the aqueous leaf extracts of Z. jujuba and I. carnea followed by challenge inoculation with R. solani induces systemic resistance in rice as evident from significantly increased accumulation of pathogenesis-related proteins such as chitinase, β-1,3-glucanase and peroxidase, as well as defense-related compounds such as phenylalanine ammonia-lyase and phenolic substances. Thin layer chromatographic separation of secondary metabolites revealed presence of alkaloid and terpenoid compounds in the leaf extracts of Z. jujuba that exhibited toxicity against R. solani under in vitro condition. Thus, the enhanced sheath blight resistance in rice seedlings treated with leaf extracts of Z. jujuba or I. carnea can be attributed to the direct inhibitory effects of these leaf extracts as well as their ability to elicit systemic resistance against R. solani.

  14. Evaluation of the effect of an organic extract obtained from Ipomoea alba L. on experimental periodontitis in rats.

    Science.gov (United States)

    Barrella, Guilherme Emerson; Suffredini, Ivana Barbosa; Ribeiro, Fernanda Vieira; Cirano, Fabiano Ribeiro; Pimentel, Suzana Peres

    2012-01-01

    The aim of this study was to evaluate the effect of an organic extract obtained from Ipomoea alba L. (Convolvulaceae or OE 1493), on experimental periodontal disease in rats. Periodontitis was induced in thirty six Wistar rats: a first mandibular molar was randomly assigned to receive a ligature, whereas the contralateral molar was left unligated. Animals were randomly assigned to two groups and treated topically, three times a day, for 11 days, as follows: Control Group - vehicle-treated (n = 18), and Test Group - OE 1493-treated (n = 18). The rats were sacrificed on the 12th day. Morphometrical measurements from the cementoenamel junction to the bone crest were performed to determine alveolar bone loss, using standardized photographs. Single- and multi-dose acute toxicity assays were carried out after OE 1493 treatment. Morphometrical analysis demonstrated that topically-administered OE 1493 showed no effect on reducing bone loss when compared with the control group (p > 0.05). In addition, OE 1493 did not present toxicity. Within the limits of this investigation, it may be concluded that OE 1493 did not show any positive influence on the progression of ligature-induced periodontitis in rats, when administered according to the regimen used in the present study.

  15. Biosynthesis and Accumulation of Ergoline Alkaloids in a Mutualistic Association between Ipomoea asarifolia (Convolvulaceae) and a Clavicipitalean Fungus1

    Science.gov (United States)

    Markert, Anne; Steffan, Nicola; Ploss, Kerstin; Hellwig, Sabine; Steiner, Ulrike; Drewke, Christel; Li, Shu-Ming; Boland, Wilhelm; Leistner, Eckhard

    2008-01-01

    Ergoline alkaloids occur in taxonomically unrelated taxa, such as fungi, belonging to the phylum Ascomycetes and higher plants of the family Convolvulaceae. The disjointed occurrence can be explained by the observation that plant-associated epibiotic clavicipitalean fungi capable of synthesizing ergoline alkaloids colonize the adaxial leaf surface of certain Convolvulaceae plant species. The fungi are seed transmitted. Their capacity to synthesize ergoline alkaloids depends on the presence of an intact differentiated host plant (e.g. Ipomoea asarifolia or Turbina corymbosa [Convolvulaceae]). Here, we present independent proof that these fungi are equipped with genetic material responsible for ergoline alkaloid biosynthesis. The gene (dmaW) for the determinant step in ergoline alkaloid biosynthesis was shown to be part of a cluster involved in ergoline alkaloid formation. The dmaW gene was overexpressed in Saccharomyces cerevisiae, the encoded DmaW protein purified to homogeneity, and characterized. Neither the gene nor the biosynthetic capacity, however, was detectable in the intact I. asarifolia or the taxonomically related T. corymbosa host plants. Both plants, however, contained the ergoline alkaloids almost exclusively, whereas alkaloids are not detectable in the associated epibiotic fungi. This indicates that a transport system may exist translocating the alkaloids from the epibiotic fungus into the plant. The association between the fungus and the plant very likely is a symbiotum in which ergoline alkaloids play an essential role. PMID:18344419

  16. Biosynthesis and accumulation of ergoline alkaloids in a mutualistic association between Ipomoea asarifolia (Convolvulaceae) and a clavicipitalean fungus.

    Science.gov (United States)

    Markert, Anne; Steffan, Nicola; Ploss, Kerstin; Hellwig, Sabine; Steiner, Ulrike; Drewke, Christel; Li, Shu-Ming; Boland, Wilhelm; Leistner, Eckhard

    2008-05-01

    Ergoline alkaloids occur in taxonomically unrelated taxa, such as fungi, belonging to the phylum Ascomycetes and higher plants of the family Convolvulaceae. The disjointed occurrence can be explained by the observation that plant-associated epibiotic clavicipitalean fungi capable of synthesizing ergoline alkaloids colonize the adaxial leaf surface of certain Convolvulaceae plant species. The fungi are seed transmitted. Their capacity to synthesize ergoline alkaloids depends on the presence of an intact differentiated host plant (e.g. Ipomoea asarifolia or Turbina corymbosa [Convolvulaceae]). Here, we present independent proof that these fungi are equipped with genetic material responsible for ergoline alkaloid biosynthesis. The gene (dmaW) for the determinant step in ergoline alkaloid biosynthesis was shown to be part of a cluster involved in ergoline alkaloid formation. The dmaW gene was overexpressed in Saccharomyces cerevisiae, the encoded DmaW protein purified to homogeneity, and characterized. Neither the gene nor the biosynthetic capacity, however, was detectable in the intact I. asarifolia or the taxonomically related T. corymbosa host plants. Both plants, however, contained the ergoline alkaloids almost exclusively, whereas alkaloids are not detectable in the associated epibiotic fungi. This indicates that a transport system may exist translocating the alkaloids from the epibiotic fungus into the plant. The association between the fungus and the plant very likely is a symbiotum in which ergoline alkaloids play an essential role.

  17. Descripción de las especies del género Ipomoea presentes en el area de riego del río Dulce, Santiago del Estero, Argentina Description of the species of the genus Ipomoea in the irrigated area of Río Dulce, Santiago del Estero, Argentina

    Directory of Open Access Journals (Sweden)

    E. del V. Carrizo

    2001-08-01

    Full Text Available El objetivo del presente trabajo fue relevar y diferenciar especies del género Ipomoea presentes en diferentes fases de desarrollo, en el área de riego del Río Dulce, Santiago del Estero, Argentina. Para ello fueron herborizados y fotografiados diversos ejemplares encontrados en la región. En el laboratorio, después del trabajo de identificación, fueron descritas las diversas fases de desarrollo de la planta: semilla, plántula y estado adulto, creándose códigos de identificación para cada fase. Se registraron ocho especies del género Ipomoea, I. alba; I. amnicola; I. cairica; I. carnea; I. grandifolia; I. nil; I. purpurea e I. quamoclit, algunas de las cuales se cultivan como ornamentales, otras aparecen como ruderales y algunas son malezas en cultivos de la región.The objective of this research was to describe and differentiate the stages of species of the genus Ipomoea in the irrigated area of Rio Dulce - Santiago del Estero - Argentina, under different growth stages. Species prototypes from the area were mounted in a herbarium, photographed and then identified with detailed descriptions of the seed, seedling and mature stages. A classification key was constructed to identify them under different growth stages. Eight species in the genus Ipomoea: (I. alba, I. amnicola, I. cairica, I. carnea, I. grandifolia, I. nil, I. purpurea and I. quamoclit were registered, some cultivated as ornamental, while others appear naturally or as crop weeds in the region.

  18. HPLC Separation and Determination of Carotenoids in Ipomoea batatas%HPLC分离测定红心薯中类胡萝卜素

    Institute of Scientific and Technical Information of China (English)

    吉宏武; 施兆鹏

    2001-01-01

    The carotenoids from Ipomoea batatas were separated and determined by HPLC. Results show that the main carotenoids in Ipomoea batatas can be successfully separated in 10min with detection at 440nm by extracting the sample in hexane -acetone-ethanol-toluene and 40% potassium hydroxide methanol for twelve hours ,employing Nova-Pak C18 stainliss steel column (3.9×150 mm),using acetonitrile -chloroform-methyl carbinol (83∶15∶2)as flow phase, applying linear gradient eluting with the rate of flow, with the temperature of column at 25 ℃.Four kinds of main carotenoids from Ipomoea batatas were determined by external standards.%应用高效液相色谱法分离和测定红心薯中类胡萝卜素.样品制备采用已烷-丙酮-乙腈-甲苯混合溶剂与40%氢氧化钾甲醇溶液浸提、皂化的方法,使用Nova-Pak C18不锈钢柱,乙腈-三氯甲烷-叔丁醇作三元流动相,流量线性梯度洗脱等色谱条件,在440 nm波长下检测,红心薯中主要类胡萝卜素得到了较好的分离.以外标定量法对红心薯中的4种主要类胡萝卜素(β-胡萝卜素,叶黄质,堇黄质,新黄质)进行了定量,结果较为满意.

  19. Efeito do déficit hídrico na eficiencia de herbicidas e nas características bioquímicas de Ipomoea grandifolia

    OpenAIRE

    Vitorino, H. S. [UNESP; D. Martins

    2012-01-01

    O objetivo deste estudo foi avaliar a eficácia dos herbicidas inibidores da ALS e PROTOX no controle de Ipomoea grandifolia sob dois níveis de déficit hídrico, bem como determinar a influência desse déficit hídrico sobre o conteúdo de carboidratos solúveis, proteínas totais e aminoácidos livres da planta daninha. O trabalho foi conduzido em casa de vegetação, no delineamento experimental inteiramente casualizado, com os tratamentos dispostos em esquema fatorial 4 x 2, sendo quatro herbicidas ...

  20. Marcadores funcionales relacionados con la síntesis de pigmentos y su localización en un mapa de ligamiento en Ipomoea batatas L. Lam

    OpenAIRE

    Arizio, Carla Marcela

    2011-01-01

    Ipomoea batatas L. Lam, es una especie de importancia agrícola, que se cultiva en más de 100 países con una producción anual de 120 millones de toneladas. Esta especie presenta una raíz engrosada denominada batata que constituye un alimento básico en muchos países subdesarrollados. En la piel y en la pulpa de la raíz engrosada puede observarse una amplia variedad de colores. Estos colores se deben a la presencia de dos clases de pigmentos: las antocianinas (flavonoides) y los carotenoides. Es...

  1. Efeito da cobertura morta de milheto (Pennisetum americanum) sobre a eficácia do herbicida metribuzin no controle de Ipomoea grandifolia e Sida rhombifolia

    OpenAIRE

    Godoy,M.C.; D.K. Meschede; C.A Carbonari; Correia, M. R.; E.D Velini

    2007-01-01

    Com o objetivo de avaliar a eficácia do metribuzin (480 g i.a. ha-1) associado à palha de milheto no controle de Ipomoea grandifolia e Sida rhombifolia, foram realizados dois experimentos em casa de vegetação. No primeiro, os tratamentos constituíram-se de diferentes posicionamentos do herbicida, aplicado sobre e sob a palha em diferentes condições de umidade. No segundo, foram estudados diferentes períodos de permanência (0, 7, 14 e 21 dias) do herbicida sobre a palha de milheto antes da oco...

  2. 超声波提取紫薯多糖的工艺优化%Optimization of Ultrasonic Extraction Process of Ipomoea batatas ( Linn. ) Lam. Polysaccharide

    Institute of Scientific and Technical Information of China (English)

    姚惠伶; 蒋林彬

    2011-01-01

    [ Objective] To optimize the extraction conditions of polysaccharide from lpomoea batatas ( Linn. ) Lam. [ Method ] Polysaccharide from Ipomoea batatas ( Linn. ) Lam. was extracted by ultrasonic technology,and the extraction conditions were optimized by orthogonal test combined with single factor test. [ Result ] The influencing order of each factor on yield of polysaccharide was determined to be ultrasonic frequency > solid to liquid ratio > extraction temperature > extraction duration. The optimum conditions for extracting polysaccharide from Ipomoea batatas ( Linn. ) Lam. were: solid to liquid ratio of 1: 15, extraction duration of 40 min, extraction temperature of 60 ℃ and ultrasonic frequency of 80 kHz. Under the optimal extracting conditions,extraction rate of polysaccharide from Ipomoea batatas (Linn.) Lam. reached as high as 3.63%.[ Conclusion] Ipomoea batatas ( Linn. ) Lam. polysaccharide extracted by ultrasonic technology was high in yield and short in extraction time. If carried out at low temperature,the extraction was less in extracting solvent and less in cost.%[目的]优化紫薯多糖的提取工艺.[方法]利用超声波技术提取紫薯粗多糖,用单因素试验和正交试验设计相结合的方法获得最佳提取工艺.[结果]影响紫薯多糖得率的主要因素按重要性排序为:超声频率>料液比>提取温度>提取时间;紫薯粗多糖最佳提取工艺条件为:料液比1:15,提取时间40min,提取温度60℃,超声频率80 kHz,此条件下紫薯多糖的提取率为3.63%.[结论]利用超声技术提取紫薯多糖可以提高多糖得率、缩短提取时间、采用较低温度提取、节省提取溶剂,降低提取成本.

  3. 西蒙1号番薯毛状根生物碱成分的研究%Alkaloids of the hairy roots of Ipomoea batatas Lam

    Institute of Scientific and Technical Information of China (English)

    袁珊琴; 赵毅民; 尤勇

    2004-01-01

    目的研究西蒙1号番薯Ipomoea batatas Lam毛状根中的生物碱成分.方法用硅胶柱色谱和薄层色谱方法进行分离纯化,根据光谱数据确定其结构.结果分离、鉴定了1个生物碱.结论该化合物为一新的吲哚类生物碱,命名为番薯碱甲(ipomine A).

  4. Eficiência fotossintética e consumo de água de Ipomoea triloba após aplicação de herbicidas Photosynthetic efficiency and water consumption of Ipomoea triloba after herbicide application

    Directory of Open Access Journals (Sweden)

    R. Araldi

    2012-09-01

    Full Text Available Este trabalho foi desenvolvido com o objetivo de avaliar a fluorescência através da taxa de transporte de elétrons, consumo de água e intoxicação de plantas de Ipomoea triloba após aplicação de quatro herbicidas de diferentes mecanismos de ação. Os herbicidas aplicados foram: glyphosate, haloxyfop-methyl, diuron e amicarbazone. A aplicação foi feita com auxílio de um pulverizador estacionário instalado em laboratório; após a aplicação dos tratamentos, as plantas foram mantidas em casa de vegetação. Foi avaliada a taxa de transporte de elétrons (ETR, o consumo de água e a intoxicação das plantas em vários períodos após o início do experimento. Os dados de ETR e fitointoxicação foram expressos em porcentagem da testemunha e submetidos à análise de variância e à comparação das médias. Quanto ao consumo de água, os dados foram acumulados e ajustados por modelos de regressão. Assim, pode-se dizer que o fluorômetro é uma ferramenta adequada para verificar a intoxicação antecipada em plantas de I. triloba tratadas com os herbicidas amicarbazone e diuron, visto que a inibição da ETR foi verificada antes de qualquer intoxicação visual sofrida por essas plantas; o consumo de água está relacionado diretamente com o transporte de elétrons, com exceção das plantas submetidas ao haloxyfop-methyl, que não sofreram interferência no transporte de elétrons, mas reduziram o consumo de água.This work was carried out to evaluate fluorescence through electron transport rate (ETR, water consumption and intoxication of Ipomoea triloba after application of four herbicides with different mechanisms of action. The herbicides glyphosate, haloxyfop-methyl, diuron, and amicarbazone were applied using a stationary sprayer installed in the laboratory. After application, the plants were kept under greenhouse conditions. Electron transport rate, water consumption and plant intoxication were evaluated at various periods

  5. Interferência de uma comunidade de plantas daninhas com predominância de Ipomoea hederifolia na cana-soca Interference of a weed community with predominance of Ipomoea hederifolia on sugar cane ratton

    Directory of Open Access Journals (Sweden)

    I.A.B. Silva

    2009-06-01

    Full Text Available Levantamentos recentes da flora de plantas daninhas associada à cultura da cana-de-açúcar apontaram Ipomoea hederifolia como uma das espécies mais importantes. Em virtude disso, objetivou-se determinar o potencial de redução da produtividade da cana-de-açúcar e da qualidade do caldo em resposta à interferência de uma comunidade de plantas daninhas com predomínio de I. hederifolia, bem como o período anterior à interferência (PAI. Foi instalado um experimento em Pitangueiras, SP, em que constituíram tratamentos nove períodos crescentes de convivência das plantas daninhas com a cultura (0, 16, 30, 44, 69, 97, 135, 160, 188 e 229 dias após o cultivo e adubação e início da brotação - DAB. O delineamento experimental foi em blocos casualizados, com cada tratamento em quatro repetições. Concluiu-se que o potencial de redução do número final de colmos e de produtividade foi de 34% e 46%, respectivamente. O PAI foi do início da brotação e se estendeu por 33 dias. Com o aumento do período de convivência com a comunidade infestante, houve antecipação do processo de maturação dos colmos, cujo caldo tendeu a apresentar maior valor de açúcar total recuperável (ATR. Com a redução de produtividade devido à interferência, o valor de ATR ha-1 tendeu a ser negativamente afetado.Recent surveys on weed community associated with sugar cane have pointed Ipomoea hederifolia as one of the most important species. Thus, this work aimed to determine the potential for reducing the productivity and quality of sugar cane in response to weed community interference with a predominance of I. hederifolia, and the period prior to interference (PPI. An assay was installed in Pitangueiras , Brazil, with the treatments consisting of nine periods of coexistence of the weeds with the culture (0, 16, 30, 44, 69,97, 135, 160,188 and 229 days after cultivation and fertilization and days after sprouting. The experiments were arranged in a

  6. Osmotic dehydration of sweet potato (Ipomoea batatas in ternary solutions Desidratação osmótica de batata-doce (Ipomoea batatas em soluções ternárias

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    Graziella Colato Antonio

    2008-09-01

    Full Text Available The aim of this work was to evaluate the osmotic dehydration of sweet potato (Ipomoea batatas using hypertonic sucrose solutions, with or without NaCl, at three different concentrations, at 40 °C. Highest water losses were obtained when the mixture of sucrose and NaCl was used. The addition of NaCl to osmotic solutions increases the driving force of the process and it is verified that the osmotic dehydration process is mainly influenced by changes in NaCl concentration, but the positive effect of the salt-sucrose interaction on soluble solids also determined the decrease of solid gain when solutes were at maximum concentrations. Mass transfer kinetics were modeled according to Peleg, Fick and Page's equations, which presented good fittings of the experimental data. Peleg's equation and Page's model presented the best fitting and showed excellent predictive capacity for water loss and salt gain data. The effective diffusivity determined using Fick's Second Law applied to slice geometry was found to be in the range from 3.82 x 10-11 to 7.46 x 10-11 m²/s for water loss and from 1.18 x 10-10 to 3.38 x 10-11 m²/s for solid gain.O objetivo deste trabalho foi avaliar a desidratação osmótica da batata-doce (Ipomoea batatas utilizando soluções hipertônicas de sacarose e/ou NaCl, em três concentrações diferentes a 40 °C. As perdas de água mais elevadas foram obtidas quando se utilizou a mistura de sacarose e NaCl. A adição do NaCl às soluções osmóticas aumenta a força motriz do processo. Verifica-se que o processo de desidratação osmótica é principalmente influenciado pela mudança da concentração de NaCl, mas o efeito positivo da interação do sal com a sacarose também determinou a diminuição do ganho de sólidos quando os solutos estavam em concentrações máximas. As cinéticas de transferência de massa foram modeladas de acordo com as Equações de Peleg, de Fick e de Page, e apresentaram bons ajustes aos dados

  7. 甘薯栽培种及其近缘野生种的DAPI核型及rDNA-FISH分析%rDNA-FISH Analysis and DAPI- karyotype of Ipomoea batatas cv.and Ipomoea hederacea Jacq

    Institute of Scientific and Technical Information of China (English)

    安婷婷; 汤佳立; 孙健英; 曹清河; 马代夫; 李宗芸

    2012-01-01

    Ipomoea batatas cv. Xushu 18 and its two wild relatives I. hederacea Jacq. from American and Hong Kong in China were studied by DAPI banding and rDNA-fluorescence in situ hybridization. The DA-PI banding showed the karyotype of I. batatas and I. hederacea Jacq. from American and Hong Kong in China are 2n=6x=90 = 72m+18sm(18SAT) with satellites on chromosome 1,3 and 6:2n = 2x=30 = 30m (4SAT);2n=2x=30 = 20m+10sm(4SAT),both with satellite on chromosome 6 and 12,respectively. The FISH data indicated that three pairs of 5S rDNA signals,located on centromere,pericentromere and the te-lomere of chromosome respectively of I. batatas (Two pairs of 45S rDNA signals, located on chromosome 6 and 12 were detected on both I. hederacea Jacq. ; While 1 pairs of 5S rDNA signals presented on chromosome 6 of I. hederacea Jacq. from Hong Kong, 2 pairs of 5S rDNA signals occurred on the chromosome 6 and 12 of that from American. Taking all the data obtained in the study, both I. hederacea Jacq. were distant from the sweet potato, and there were some differentiations in the chromosomes of the I. hederacea Jacq. from different territories.%利用DAPI显带和rDNA-FISH技术对栽培种甘薯(‘徐薯18’)(Jpomoea batatas cv.Xushu No.18)及2种不同产地近缘野生种(Ipomoea hederacea Jacq.)进行了细胞遗传学研究.DAPI核型分析表明,‘徐薯18’核型公式为2n=6x=90=72m+18sm(18SAT),随体位于第1、3、6染色体上;美国近缘野生种核型公式为2n=2x=30=30m(4SAT),香港近缘野生种核型公式为2n=2x=30=20m+ 10sm(4SAT),随体均位于第6、12染色体上.rDNA-FISH结果显示,栽培种甘薯基因组中含有3对5S rDNA位点,分别位于着丝粒区、亚着丝粒区和染色体端部;美国近缘野生种基因组中含有2对5S rDNA位点,香港近缘野生种基因组中含有1对5S rDNA位点,均位于随体部位;两种不同地域来源的近缘野生种基因组中均含有2对45S rDNA位点,分别位于第6和第12染色体上.

  8. Efeito do período de chuva no controle de Euphorbia heterophylla e Ipomoea purpurea pelos herbicidas glyphosate e sulfosate Effect of rainfall on Euphorbia heterophylla and Ipomoea purpurea control by glyphosate and sulfosate

    Directory of Open Access Journals (Sweden)

    P.A. Monquero

    2007-01-01

    Full Text Available Avaliou-se a influência do período de chuva sobre a eficácia dos herbicidas sulfosate e glyphosate no controle de Euphorbia heterophylla e Ipomoea purpurea. O experimento foi instalado em casa de vegetação, no Centro de Ciências Agrárias/UFSCar, em Araras, SP. Os tratamentos, além da testemunha, consistiram na aplicação de duas doses em equivalente ácido dos herbicidas sulfosate (0,66 e 1,32 kg ha-1 e glyphosate (0,72 e 1,44 kg ha-1, sendo as plantas tratadas submetidas à simulação de chuva de 20 mm durante 30 minutos, nos intervalos de 2, 4, 6, 8 e 48 horas após a aplicação. O delineamento experimental utilizado foi o inteiramente casualizado, com quatro repetições. A eficácia no controle das plantas daninhas foi avaliada 7, 14 e 21 dias após a aplicação dos tratamentos (DAT e a matéria seca, aos 28 DAT. E. heterophylla não foi eficazmente controlada pelo herbicida glyphosate, exigindo intervalo sem chuva superior a 24 horas após a aplicação para obter, na maior dose, controle de apenas 60%. Por sua vez, o herbicida sulfosate proporcionou controle de 75%, mesmo na menor dose, quando a chuva ocorreu quatro horas depois da aplicação. I. purpurea também não foi controlada de maneira eficaz pelo herbicida glyphosate, independentemente da dose e do intervalo de chuva; no entanto, somente a maior dose do herbicida sulfosate foi eficiente no controle desta espécie, a partir de quatro horas sem chuva. Para ambas as espécies, o herbicida sulfosate foi menos afetado pelo período da chuva.The influence of rainfall occurrence was evaluated on the efficacy of the herbicides glyphosate and sulfosate applied in post-emergence on Euphorbia heterophylla and Ipomoea purpurea. The experiment was conducted under greenhouse conditions at the Centro de Ciências Agrárias/UFSCar, in Araras-SP, Brazil. The treatments,plus control, consisted of two doses, in acid equivalent of sulfosate (0.66 and 1.32 kg ha-1 and glyphosate (0.72 and

  9. Germinação de sementes de plantas daninhas de pastagens cultivadas: Mimosa pudica e Ipomoea asarifolia Seed germination of weeds from cultivated pasture areas: Mimosa pudica and Ipomoea asarifolia

    Directory of Open Access Journals (Sweden)

    A.P.S. Souza Filho

    2001-04-01

    Full Text Available Malícia (Mimosa pudica e salsa (Ipomoea asarifolia são importantes plantas daninhas que infestam áreas de pastagens cultivadas da região amazônica brasileira. Neste trabalho foram analisados os efeitos de fatores relacionados ao solo e clima na variação da germinação (percentual e índice de velocidade de germinação - IVG de sementes dessas espécies. A germinação foi monitorada em um período de 15 dias, com contagens diárias e eliminação das sementes germinadas. Os resultados mostraram que os fatores de solo alumínio, pH, cálcio e magnésio não influenciam a germinação das duas espécies de plantas daninhas. A germinação das sementes de malícia e salsa pode ocorrer tanto na presença como na ausência de luz. A temperatura ótima de germinação (percentual e IVG para ambas as espécies foi de 30 ºC contínua e 25-35 ºC alternada, embora as sementes de malícia tenham germinado satisfatoriamente em temperaturas contínuas superiores e inferiores a 30 ºC e em todas as combinações de temperaturas alternadas. Comparativamente, o índice de velocidade de germinação (IVG foi mais sensível aos efeitos da temperatura do que o percentual de germinação. Ambas as espécies responderam negativamente ao aumento da salinidade, sendo a espécie malícia mais tolerante ao sal do que a salsa.Mimosa pudica and Ipomoea asarifolia are important weeds of cultivated pasture in the Brazilian Amazon Region. This research analyzes the effects of the change in soil and climate factors on the germination (percentage and germination speed index of these species. Germination was monitored for a 15-day period, with daily counting and elimination of the germinated seeds. The results showed that soil factors such as aluminum, pH, calcium, and magnesium did not influence the germination of the two weed species. The germination of both M. pudica and I. asarifolia is independent of light exposure. Optimum germination (percentage and

  10. Anti-hepatotoxic and antioxidant influence of Ipomoea carnea against anti-tubercular drugs induced acute hepatopathy in experimental rodents

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    Ramesh Kumar Gupta

    2013-11-01

    Full Text Available Objective: To assess the hepatoprotective effect of Ipomoea carnea (I. carnea extract against antitubercular drug-induced liver toxicity in experimental animals. Methods: I. carnea extracts (125, 250 and 500 mg/kg, p.o. body weight were administered daily for 35 d in experimental animals. Liver toxicity was induced by combination of three antitubercular drugs (isoniazid 7.5 mg/kg, rifampicin 10 mg/kg and pyrazinamide 35 mg/kg given orally as suspension for 35 d in rats. Treatment groups received I. carnea extracts along with antitubercular drugs. The hepatoprotective activity was assessed using various biochemical parameters like aspartate aminotransferase, alanine aminotransferase, alkaline phosphatise and total bilirubin. Meanwhile, in-vivo antioxidant activities as lipid peroxidation, reduced glutathione, superoxide dismutase and catalase were measured in rat liver homogenate along with ATPase and G-6-Pase. The biochemical observations were supplemented by histopathological examination. Results: Obtained results demonstrated that treatment with I. carnea extracts significantly (P<0.05-P<0.001 and dose-dependently prevented drug induced increase in serum levels of hepatic enzymes. Furthermore, I. carnea extracts significantly (up to P<0.001 reduced the lipid peroxidation in the liver tissue and restored activities of defence antioxidant enzymes, reduced glutathione, superoxide dismutase and catalase towards normal levels. Histopathology of the liver tissue showed that I. carnea extracts attenuated the hepatocellular necrosis, massive fatty changes and led to reduction in inflammatory cells infiltration. Conclusions: The results of this study strongly indicate the protective effect of I. carnea extracts against liver injury, which may be attributed to its hepatoprotective activity, and there by scientifically support its traditional use.

  11. Influence of diesel contamination in soil on growth and dry matter partitioning of Lactuca sativa and Ipomoea batatas.

    Science.gov (United States)

    Fatokun, Kayode; Zharare, Godfrey Elijah

    2015-09-01

    Phytotoxic effect of diesel contaminated soil was investigated on growth and dry matter partitioning in Lactuca sativa and Ipomoea batatas in greenhouse pot experiment at two concentration range (0-30 ml and 0-6 ml diesel kg(-1) soil) for 14 weeks. The results indicated thatwhole plant biomass, stem length, root length, number of leaves and leaf chlorophyll in two plants were negatively correlated with increasing diesel concentrations. The critical concentration of diesel associated with 10% decrease in plant growth was 0.33 ml for lettuce and 1.50 ml for sweet potato. Thus, growth of lettuce in diesel contaminated soil was more sensitive than sweet potato. The pattern of dry matter partitioning between root and shoot in both plants were similar. In 0-6 ml diesel contamination range, allocation of dry matter to shoot system was favoured resulting in high shoot: root ratio of 4.54 and 12.91 for lettuce and sweet potato respectively. However, in 0-30 ml diesel contamination range, allocation of dry matter to root was favoured, which may have been an adaptive mechanism in which the root system was used for storage in addition to increasing the capacity for foraging for mineral nutrients and water. Although lettuce accumulated more metals in its tissue than sweet potato, the tissue mineral nutrients in both species did not vary to great extent. The critical diesel concentration for toxicity suggested that the cause of mortality and poor growth of sweet potato and lettuce grown in diesel contaminated soil was due to presence of hydrocarbons in diesel.

  12. Improved tolerance to various abiotic stresses in transgenic sweet potato (Ipomoea batatas expressing spinach betaine aldehyde dehydrogenase.

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    Weijuan Fan

    Full Text Available Abiotic stresses are critical delimiters for the increased productivity and cultivation expansion of sweet potato (Ipomoea batatas, a root crop with worldwide importance. The increased production of glycine betaine (GB improves plant tolerance to various abiotic stresses without strong phenotypic changes, providing a feasible approach to improve stable yield production under unfavorable conditions. The gene encoding betaine aldehyde dehydrogenase (BADH is involved in the biosynthesis of GB in plants, and the accumulation of GB by the heterologous overexpression of BADH improves abiotic stress tolerance in plants. This study is to improve sweet potato, a GB accumulator, resistant to multiple abiotic stresses by promoted GB biosynthesis. A chloroplastic BADH gene from Spinacia oleracea (SoBADH was introduced into the sweet potato cultivar Sushu-2 via Agrobacterium-mediated transformation. The overexpression of SoBADH in the transgenic sweet potato improved tolerance to various abiotic stresses, including salt, oxidative stress, and low temperature. The increased BADH activity and GB accumulation in the transgenic plant lines under normal and multiple environmental stresses resulted in increased protection against cell damage through the maintenance of cell membrane integrity, stronger photosynthetic activity, reduced reactive oxygen species (ROS production, and induction or activation of ROS scavenging by the increased activity of free radical-scavenging enzymes. The increased proline accumulation and systemic upregulation of many ROS-scavenging genes in stress-treated transgenic plants also indicated that GB accumulation might stimulate the ROS-scavenging system and proline biosynthesis via an integrative mechanism. This study demonstrates that the enhancement of GB biosynthesis in sweet potato is an effective and feasible approach to improve its tolerance to multiple abiotic stresses without causing phenotypic defects. This strategy for trait

  13. Digital gene expression analysis based on integrated de novo transcriptome assembly of sweet potato [Ipomoea batatas (L. Lam].

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    Xiang Tao

    Full Text Available BACKGROUND: Sweet potato (Ipomoea batatas L. [Lam.] ranks among the top six most important food crops in the world. It is widely grown throughout the world with high and stable yield, strong adaptability, rich nutrient content, and multiple uses. However, little is known about the molecular biology of this important non-model organism due to lack of genomic resources. Hence, studies based on high-throughput sequencing technologies are needed to get a comprehensive and integrated genomic resource and better understanding of gene expression patterns in different tissues and at various developmental stages. METHODOLOGY/PRINCIPAL FINDINGS: Illumina paired-end (PE RNA-Sequencing was performed, and generated 48.7 million of 75 bp PE reads. These reads were de novo assembled into 128,052 transcripts (≥ 100 bp, which correspond to 41.1 million base pairs, by using a combined assembly strategy. Transcripts were annotated by Blast2GO and 51,763 transcripts got BLASTX hits, in which 39,677 transcripts have GO terms and 14,117 have ECs that are associated with 147 KEGG pathways. Furthermore, transcriptome differences of seven tissues were analyzed by using Illumina digital gene expression (DGE tag profiling and numerous differentially and specifically expressed transcripts were identified. Moreover, the expression characteristics of genes involved in viral genomes, starch metabolism and potential stress tolerance and insect resistance were also identified. CONCLUSIONS/SIGNIFICANCE: The combined de novo transcriptome assembly strategy can be applied to other organisms whose reference genomes are not available. The data provided here represent the most comprehensive and integrated genomic resources for cloning and identifying genes of interest in sweet potato. Characterization of sweet potato transcriptome provides an effective tool for better understanding the molecular mechanisms of cellular processes including development of leaves and storage roots

  14. Anti-hepatotoxic and antioxidant influence of Ipomoea carnea against anti-tubercular drugs induced acute hepatopathy in experimental rodents

    Institute of Scientific and Technical Information of China (English)

    Ramesh Kumar Gupta; Ashok Kumar Gupta; Sudhansu Ranjan Swain; Vaishali; Gaurav Gupta; Saifuddin Khalid; Didagi Kulkarni Suresh; Rajnish Kumar Singh

    2013-01-01

    Objective: To assess the hepatoprotective effect of Ipomoea carnea (I. carnea) extract against antitubercular drug-induced liver toxicity in experimental animals. Methods: I. carnea extracts (125, 250 and 500 mg/kg, p.o. body weight) were administered daily for 3d5r udg sin experimental animals. Liver toxicity was induced by combination of three antitubercular suspen(siisoonn ifaozr id 7.5 mg/kg, rifampicin 10 mg/kg and pyrazinamide 35 mg/kg) given orally as drugs. 35 d in rats. Treatment groups received I. carnea extracts along with antitubercular aspartaTteh ea mhienpoattroapnrsofeteracstiev,e a alacntiivniet ya mwainso atrsasnessfseerda sues, ianlgk avlainrieo upsh obsipochhaetimsei caanl dp atortaaml ebtielirrsu bliikne. dMiesmanuwtahsiele a, nind- cvaivtaol aasnet iwoxeirdea mnte aascutirveidti eisn aras t lliipviedr pheormoxoigdeantaioten ,a rloendgu cweidth g lutathione, superoxide ATPase and G-6-Pase. The biochemical observations were supplemented by histopathological examination. Results: Obtained results demonstrated that treatment with I. carnea extracts significantly h(Pe

  15. De novo transcriptome assembly of Ipomoea nil using Illumina sequencing for gene discovery and SSR marker identification.

    Science.gov (United States)

    Wei, Changhe; Tao, Xiang; Li, Ming; He, Bin; Yan, Lang; Tan, Xuemei; Zhang, Yizheng

    2015-10-01

    Ipomoea nil is widely used as an ornamental plant due to its abundance of flower color, but the limited transcriptome and genomic data hinder research on it. Using illumina platform, transcriptome profiling of I. nil was performed through high-throughput sequencing, which was proven to be a rapid and cost-effective means to characterize gene content. Our goal is to use the resulting information to facilitate the relevant research on flowering and flower color formation in I. nil. In total, 268 million unique illumina RNA-Seq reads were produced and used in the transcriptome assembly. These reads were assembled into 220,117 contigs, of which 137,307 contigs were annotated using the GO and KEGG database. Based on the result of functional annotations, a total of 89,781 contigs were assigned 455,335 GO term annotations. Meanwhile, 17,418 contigs were identified with pathway annotation and they were functionally assigned to 144 KEGG pathways. Our transcriptome revealed at least 55 contigs as probably flowering-related genes in I. nil, and we also identified 25 contigs that encode key enzymes in the phenylpropanoid biosynthesis pathway. Based on the analysis relating to gene expression profiles, in the phenylpropanoid biosynthesis pathway of I. nil, the repression of lignin biosynthesis might lead to the redirection of the metabolic flux into anthocyanin biosynthesis. This may be the most likely reason that I. nil has high anthocyanins content, especially in its flowers. Additionally, 15,537 simple sequence repeats (SSRs) were detected using the MISA software, and these SSRs will undoubtedly benefit future breeding work. Moreover, the information uncovered in this study will also serve as a valuable resource for understanding the flowering and flower color formation mechanisms in I. nil.

  16. On the origin of sweet potato (Ipomoea batatas (L.) Lam.) genetic diversity in New Guinea, a secondary centre of diversity.

    Science.gov (United States)

    Roullier, C; Kambouo, R; Paofa, J; McKey, D; Lebot, V

    2013-06-01

    New Guinea is considered the most important secondary centre of diversity for sweet potato (Ipomoea batatas). We analysed nuclear and chloroplast genetic diversity of 417 New Guinea sweet potato landraces, representing agro-morphological diversity collected throughout the island, and compared this diversity with that in tropical America. The molecular data reveal moderate diversity across all accessions analysed, lower than that found in tropical America. Nuclear data confirm previous results, suggesting that New Guinea landraces are principally derived from the Northern neotropical genepool (Camote and Batata lines, from the Caribbean and Central America). However, chloroplast data suggest that South American clones (early Kumara line clones or, more probably, later reintroductions) were also introduced into New Guinea and then recombined with existing genotypes. The frequency distribution of pairwise distances between New Guinea landraces suggests that sexual reproduction, rather than somaclonal variation, has played a predominant role in the diversification of sweet potato. The frequent incorporation of plants issued from true seed by farmers, and the geographical and cultural barriers constraining crop diffusion in this topographically and linguistically heterogeneous island, has led to the accumulation of an impressive number of variants. As the diversification of sweet potato in New Guinea is primarily the result of farmers' management of the reproductive biology of their crop, we argue that on-farm conservation programmes that implement distribution of core samples (clones representing the useful diversity of the species) and promote on-farm selection of locally adapted variants may allow local communities to fashion relatively autonomous strategies for coping with ongoing global change.

  17. Application of IBA in environmental remediation using skins of cocoa (Theobroma cacao) and sweet potatoes (Ipomoea batatas)

    Energy Technology Data Exchange (ETDEWEB)

    Sumbu, K. [Department of Chemistry, Cape Peninsula University of Technology, PO Box 1906, Bellville, 7535 (South Africa); Mars, J.A.; Gihwala, D. [Department of Biomedical Sciences, Cape Peninsula University of Technology, PO Box 1906, Bellville, 7535 (South Africa)

    2013-07-01

    Full text: The industrial sector is one of the most dynamic sectors of the economy and is of cardinal importance in economic development and poverty alleviation. Furthermore, economies with low levels of industrialisation are gradually shifting dependence from agriculture to the industrial sector, while developed economies, with a high level of industrialisation, are shifting from the industrial to the service sector [1]. Amongst the various industrial sectors, a substantial portion of effluents containing heavy metals are generated by electroplating (nickel, lead, zinc and copper), tanneries (chrome) and other chemical industries. Many engineering processes have been designed to remove the heavy metals from the polluted parts of the ecosystem. These processes are however highly capital intensive. To establish a less costly means of removal of heavy metal pollution, the dried skins of cocoa (Theobroma cacao) and sweet potato (Ipomoea batatas) were pulverized and used in the absorption of the heavy metals Ni and Pb. After adsorption by the metals, the powders were dried and then pressed into tablets. For quantification of the elemental adsorption, PIXE, using the Geo PIXE computational software, is a versatile multi-element analytical technique, and has minimum detection limits down to concentration ranges as low as 0.1 parts per million [2,3]. To determine the composition of the major elements such as C, O and N, Backscattering Spectrometry, using SimNRA software, [4] was used. In this study we report on the economic viability of using the skins of cocoa and sweet potato in the removal of Ni and Pd from polluted waters. (author)

  18. An Ipomoea batatas iron-sulfur cluster scaffold protein gene, IbNFU1, is involved in salt tolerance.

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    Degao Liu

    Full Text Available Iron-sulfur cluster biosynthesis involving the nitrogen fixation (Nif proteins has been proposed as a general mechanism acting in various organisms. NifU-like protein may play an important role in protecting plants against abiotic and biotic stresses. An iron-sulfur cluster scaffold protein gene, IbNFU1, was isolated from a salt-tolerant sweetpotato (Ipomoea batatas (L. Lam. line LM79 in our previous study, but its role in sweetpotato stress tolerance was not investigated. In the present study, the IbNFU1 gene was introduced into a salt-sensitive sweetpotato cv. Lizixiang to characterize its function in salt tolerance. The IbNFU1-overexpressing sweetpotato plants exhibited significantly higher salt tolerance compared with the wild-type. Proline and reduced ascorbate content were significantly increased, whereas malonaldehyde (MDA content was significantly decreased in the transgenic plants. The activities of superoxide dismutase (SOD and photosynthesis were significantly enhanced in the transgenic plants. H2O2 was also found to be significantly less accumulated in the transgenic plants than in the wild-type. Overexpression of IbNFU1 up-regulated pyrroline-5-carboxylate synthase (P5CS and pyrroline-5-carboxylate reductase (P5CR genes under salt stress. The systemic up-regulation of reactive oxygen species (ROS scavenging genes was found in the transgenic plants under salt stress. These findings suggest that IbNFU1gene is involved in sweetpotato salt tolerance and enhances salt tolerance of the transgenic sweetpotato plants by regulating osmotic balance, protecting membrane integrity and photosynthesis and activating ROS scavenging system.

  19. Alkaloids from the poisonous plant Ipomoea carnea: effects on intracellular lysosomal glycosidase activities in human lymphoblast cultures.

    Science.gov (United States)

    Ikeda, Kyoko; Kato, Atsushi; Adachi, Isao; Haraguchi, Mitsue; Asano, Naoki

    2003-12-17

    There is natural intoxication of livestock by the ingestion of Ipomoea carnea (Convolvulaceae) in Brazil and other parts of the world. The alkaloidal glycosidase inhibitors swainsonine, 2-epi-lentiginosine, and calystegines B(1), B(2), B(3), and C(1) have been identified as constituents of this plant. Swainsonine is a potent inhibitor of rat lysosomal alpha-mannosidase, with an IC(50) value of 0.02 microM, whereas calystegines B(1), B(2), and C(1) are potent inhibitors of rat lysosomal beta-glucosidase, with IC(50) values of 2.1, 0.75, and 0.84 microM, respectively. The action of swainsonine results in a lysosomal storage disorder that closely mimics alpha-mannosidosis in humans. To determine whether the toxicity of I. carnea to livestock is due to purely swainsonine or due to a combination of effects by swainsonine and calystegines, intracellular lysosomal glycosidase activities in normal human lymphoblasts grown with inhibitors in the medium were examined. Incubation of lymphoblasts with 0.1 microM swainsonine for 3 days resulted in approximately 60% reduction of alpha-mannosidase activity. On the other hand, calystegines B(2) and C(1) showed no inhibition of beta-glucosidase up to 1 mM; instead inclusion of calystegines B(2) and C(1) at 100 microM in the culture medium increased its activity by 1.5- and 1.6-fold, respectively. Calystegines B(2) and C(1) seem to act as chemical chaperones, enhancing correct folding of the enzyme and enabling smooth trafficking to the lysosome. The lysosomal beta-glucosidase inhibitory calystegines seem to have little risk of inducing intoxication of livestock.

  20. GENOTYPIC VARIABILITY ESTIMATES OF AGRONOMIC TRAITS FOR SELECTION IN A SWEETPOTATO (IPOMOEA BATATAS POLYCROSS POPULATION IN PAPUA NEW GUINEA

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    Boney Wera

    2015-07-01

    Full Text Available Successful crop breeding program incorporating agronomic and consumer preferred traits can be achieved by recognizing the existence and degree of variability among sweetpotato (Ipomoea batatas, (L. Lam. genotypes. Understanding genetic variability, genotypic and phenotypic correlation and inheritance among agronomic traits is fundamental to improvement of any crop. The study was carried out with the objective to estimate the genotypic variability and other yield related traits of highlands sweetpotato in Papua New Guinea in a polycross population. A total of 8 genotypes of sweetpotato derived from the polycross were considered in two cycles of replicated field experiments. Analysis of Variance was computed to contrast the variability within the selected genotypes based on high yielding β-carotene rich orange-fleshed sweetpotato. The results revealed significant differences among the genotypes. Genotypic coefficient of variation (GCV % was lower than phenotypic coefficient of variation (PCV % for all traits studied. Relatively high genetic variance, along with high heritability and expected genetic advances were observed in NMTN and ABYield. Harvest index (HI, scab and gall mite damage scores had heritability of 67%, 66% and 37% respectively. Marketable tuber yield (MTYield and total tuber yield (TTYield had lower genetic variance, low heritability and low genetic advance. There is need to investigate correlated inheritance among these traits. Selecting directly for yield improvement in polycross population may not be very efficient as indicated by the results. Therefore, it can be conclude that the variability within sweetpotato genotypes collected from polycross population in Aiyura Research Station for tuber yield is low and the extent of its yield improvement is narrow.

  1. Evaluation sensorielle du couscous de farine de manioc (Manihot esculenta, Crantz substituée par celle de patate douce (Ipomoea batatas, Lam

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    Amadou, NM.

    2016-01-01

    Full Text Available Consumer Acceptance of Cassava (Manihot esculenta, Crantz flour's fufu substituted by Sweet Potato (Ipomoea batatas, Lam Flour. This study investigated the consumer acceptance of fufu made by substituting cassava (Manihot esculenta, Crantz flour with sweet potato (Ipomoea batatas, Lam flour at the Food Technology and Post-harvest laboratory of IRAD in Bambui, Cameroon. Four samples of Fufu flour samples F0, F1, F2 and F3 obtained by substituting cassava flour with 0%, 20%, 40% and 50% potato flour respectively were compared. A panel of 30 persons (habitual consumers of Cassava fufu flour carried out a sensory evaluation of the four samples and gave their level of appreciation with regards to taste, aroma, colour, and texture. The study showed that, the substitution of cassava flour with sweet potato flour had no effect on the aroma of the fufu. The sample containing 20% of sweet potato flour (F1 showed no significant difference in texture and aroma compared (p>0.05 to the control (F0. It was also considered as having the best taste (p<0.05 and was the sample preferred by the panelists. In conclusion, an increase in the proportion of sweet potato flour in the cassava flour had a negative influence on the colour, texture, taste and on the acceptance of the fufu.

  2. Reproductive allocation and output in herbaceous annuals of the genera Polygonum, Ipomoea, and Cassia in elevated CO[sub 2] environments

    Energy Technology Data Exchange (ETDEWEB)

    Farnsworth, E.J.; Bazzaz, F.A. (Harvard Univ., Cambridge, MA (United States))

    1994-06-01

    In assessing the capacity of plants to adapt to rapidly changing global climate, we must elucidate the impacts of elevated carbon dioxide on reproduction, fitness and evolution. We investigated how elevated CO[sub 2] influenced reproduction and growth of plants exhibiting a range of floral displays, the implications of shifts in allocation for fitness in these species, and whether related taxa would show similar patterns of response. Three herbaceous, annual species each of the genera Polygonum, Ipomoea, and Cassia were grown under 350 or 700 ppm CO[sub 2]. Vegetative growth and reproductive output were non-destructively measured throughout the full life span, and biomass calibrated with a subsample harvest at first flowering. Viability and germination studies of seed progeny were conducted to more precisely characterize fitness. Timecourse and numbers of floral buds, flowers, unripe and abscised fruits differed between CO[sub 2] treatments. Genera differed significantly in their phenological responses to elevated CO[sub 2], Polygonum and Cassia species (but not Ipomoea) showed accelerated, enhanced reproduction. Elevated CO[sub 2] ameliorated trade-offs between vegetative and floral production. However, seed [open quotes]quality[close quotes] and fitness were not always directly correlated with quantity produced. Species within general responded more consistently to CO[sub 2], indicating that phylogeny and life form may be general predictors of performance under global change.

  3. Intoxicação natural por Ipomoea asarifolia (Convolvulaceae em búfalos na Ilha de Marajó, Pará

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    José Diomedes Barbosa

    2012-09-01

    Full Text Available Descrevem-se os aspectos epidemiológicos e os sinais clínicos dos primeiros casos de intoxicação natural por Ipomoea asarifolia em búfalos. A doença foi diagnosticada em quatro bubalinos de três diferentes propriedades, no município de Cachoeira do Arari, Ilha de Marajó, PA, e ocorreu nos meses de novembro e dezembro, o período mais seco do ano nesta região e de escassez de alimento. Os sinais clínicos observados foram relacionados ao sistema nervoso central, como andar trôpego, hipermetria, acentuados tremores musculares, queda ao solo em posições incomuns, nistagmo e marcada excitação, sinais que se agravavam após movimentação. Baseado nos aspectos epidemiológicos, sinais clínicos e na ausência de leões histopatológicas, concluiu se tratar de intoxicação por Ipomoea asarifolia.

  4. Germinação de sementes de populações de orelha-de-mula (Alisma Plantago-Aquatica resistentes e susceptíveis ao bensulfurão-metilo Seed germination of waterplantain (Alisma Plantago-Aquatica populations resistant and susceptible to bensulfuron-methyl

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    Isabel Calha

    2008-01-01

    Baixo Mondego (6 samples; Sorraia river valey (2 samples and Sado river valey (2 samples were compared at 15 ºC and 15/30 ºC. All seed samples were two years old and an extra sample three years old was also included to assess the effect of seed age on germination. Three year seeds reached higher germination rates than two year seeds at both temperature conditions. Cumulative germination characterized for each biotype using non linear regression (SSlogis was different according to geographic origin, resistance trait and seed age. The model could not fit data from Sado S biotype and two Baixo Mondego R biotypes due to immature seed. Samples with the highest cumulative germination also have the highest germination rate. Seed germination of S populations was similar at both temperature conditions, whilst for R populations seed germination was favoured by different temperature depending on geographic origin: temperature of 15/30 ºC gave the best results for Mondego river valley (Centre region and temperature of 15 ºC for Sorraia and Sado river valleys (Centre-South region of Portugal. The resistant trait affects germination of A. plantago-aquatica seeds and may affect resistant-weed management strategies implemented early in the growing season when temperatures are lower.

  5. In vitro antiplasmodial effiacy of mangrove plant, Ipomoea pes-caprae against Plasmodium falciparum (3D7 strain

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    Veera Venkata Satish Pothula

    2015-12-01

    Full Text Available Objective: To evaluate the antiplasmodial activity of mangrove plant, Ipomoea pes-caprae (I. pes-caprae (leaves, stems, flowers and roots against chloroquine-sensitive Plasmodium falciparum (3D7 strain (P. falciparum and cytotoxicity against brine shrimp larvae and THP-1 cell line. Methods: The plants (I. pes-caprae were collected from Machilipatnam mangrove forest (latitude 16°17′ N and longitude 81 °13′ E of Krishna District, Andhra Pradesh, India. Crude extracts from dried leaves, stems, flowers and roots of I. pes-caprae were prepared through Soxhlet extraction using methanol, chloroform, hexane, ethyl acetate and aqueous sequentially. These extracts were tested in vitro against P. falciparum (3D7 strain adopted in laboratory. The crude extracts were also tested for their cytotoxicity against brine shrimp larvae and THP-1 cell line. The phytochemical screenings were also conducted with standard methods. As the part of the study, the extracts were checked for any chemical injury to erythrocytes. Results: Out of these extracts, methanolic and aqueous extracts of all plant parts and chloroform extract of stems were active, and the ethyl acetate extracts were weekly active against P. falciparum. The extracts of chloroform (except stems and hexane were inactive. Amongst these extracts, the methanolic extract of root showed excellent antimalarial activity with the IC 50 value of 15.00 µg/mL. Cytotoxic evaluation revealed that methanolic, aqueous and ethyl acetate extracts were slightly cytotoxic whereas chloroform and hexane extracts were more toxic against brine shrimp. All extracts were non-toxic to THP-1 cells. The chemical injury to erythrocytes was also evaluated and it did not show any morphological changes in erythrocytes due to the effect of plant extracts of I. pes-caprae after 48 h of incubation. The phytochemical screening of the extracts revealed the presence of alkaloids, triterpenes, flavonoids, tannins, coumarins

  6. Morphological and physiological responses of morning glory (Ipomoea lacunosa L.) grown in a lead- and chelate-amended soil.

    Science.gov (United States)

    Kambhampati, Murty S; Begonia, Gregorio B; Begonia, Maria F T; Bufford, Yolanda

    2005-08-01

    Lead (Pb) is one of the most toxic metals in the environment and may cause drastic morphological and physiological deformities in Ipomoea lacunosa. The goal of this research was to evaluate some morphological and physiological responses of morning glory grown on a Pb- and chelate-amended soil. Soil samples were analyzed, at Mississippi State University Soil Laboratory, for physico-chemical parameters, such as soil texture (73% sand, 23% silt, 4.4% clay), organic matter (6.24 +/- 0.60%), and pH (7.95 +/- 0.03), to establish soil conditions at the beginning of the experiments. Five EDTA (ethylenediaminetetraacetic acid) concentrations (0, 0.1, 0.5, 1, 5mM) and four lead (0, 500, 1000, 2000mg/L) treatments were arranged in factorial in a Randomized Complete Block (RCB) design with five replications. Duncan's multiple comparison range test showed that the mean difference values of stomatal density were significant between 500 and 1000mg/L Pb and between 1000 and 2000mg/L Pb. Two way ANOVA (at 1% level) indicated that interaction between Pb and EDTA had a significant effect on the stomatal density and photosynthetic rates, and at 5% level Pb had a significant effect on chlorophyll concentrations. Lowest concentrations of chlorophyll were recorded at 2000mg/L Pb and 5mM EDTA and exhibited a decreasing trend specifically in the ranges of 1000 and 2000mg/L Pb and 1.0 and 5.0mM EDTA. Duncan's multiple comparison range test confirmed that mean differences between the control treatment vs. 2000mg/L Pb, and 500mg/L vs. 2000mg/L Pb were significantly different atp>0.05. There was a decrease in leaf net photosynthetic rate with increasing concentrations of Pb from 0 to 2000mg/L. In conclusion, I. lacunosa L. plants were grown to maturity in all treatments with no significant and/or apparent morphological disorders, which indicated that this species might be highly tolerant even at 2000mg/L Pb concentrations in the soil.

  7. Intoxicação por Ipomoea carnea subsp. fistulosa (Convolvulaceae em caprinos na Ilha do Marajó, Pará Poisoning by Ipomoea carnea subsp. fistulosa in goats in the Marajó island, Pará

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    Carlos Alberto Oliveira

    2009-07-01

    Full Text Available Ipomoea carnea subsp. fistulosa é uma planta que contém swainsonina causando doença de depósito lisossomal em ruminantes, principalmente em caprinos na região Nordeste do Brasil. Para o estudo das plantas tóxicas da Ilha de Marajó, foram visitadas sete propriedades rurais na Ilha de Marajó, seis localizadas no município de Cachoeira do Arari e uma no município de Soure. Em todas as propriedades visitadas as pastagens eram constituídas de campo nativo, tinham pouca disponibilidade de forragem e I. carnea subsp. fistulosa encontrava-se em grande quantidade. Nas três propriedades onde eram criados caprinos foram observados animais com sinais nervosos, incluindo tremores de intenção, aumento da base de sustentação quando em estação, ataxia, hipermetria, nistagmo, paresia espástica ou debilidade, alterações posturais, perda de equilíbrio e quedas. Em duas fazendas a prevalência foi de 32% (23/71 e 100% (32/32 e em outra havia um animal com sinais acentuados e o resto do rebanho, de 19 caprinos, não foi examinado clinicamente. Bovinos, ovinos e bubalinos não foram afetados. Foram eutanasiados e necropsiados seis caprinos que apresentavam sinais clínicos acentuados. Macroscopicamente não foram observadas alterações. Na histologia observou-se vacuolização do pericário de neurônios e do citoplasma de células epiteliais da tireóide, rim, fígado, pâncreas e macrófagos de diversos órgãos. No sistema nervoso central a vacuolização era mais grave nos neurônios de Purkinje do cerebelo e nos neurônios dos núcleos cerebelares e do tronco encefálico. Observaram-se também degeneração walleriana dos axônios e gliose. A alta freqüência da intoxicação nas três fazendas que criavam caprinos sugere que a intoxicação por I. carnea subsp. fistulosa é muito importante para caprinos na Ilha de Marajó, onde há abundante quantidade da planta, que permanece verde durante todo o período seco.Ipomoea carnea subsp

  8. Controle químico de plantas dos gêneros Ipomoea e Merremia em cana-soca Chemical control of plants of the genera Ipomoea and Merremia in sugarcane

    Directory of Open Access Journals (Sweden)

    N.M. Correia

    2010-01-01

    Full Text Available Objetivou-se com este trabalho avaliar a eficácia de herbicidas aplicados em pós-emergência, isolados ou em misturas, no controle de Ipomoea grandifolia, I. hederifolia, I. purpurea, I. quamoclit, Merremia aegyptia e M. cissoides na cultura da cana-de-açúcar. O experimento foi desenvolvido no período de novembro de 2007 a julho de 2008, em área de produção comercial de cana-de-açúcar localizada no município de Jaboticabal, SP. Foram avaliados seis tratamentos com herbicidas [trifloxysulfuron + ametryn (37 + 1.463,07 g ha-1, diuron + hexazinone (1.170 + 330 g ha-1, metribuzin (1.920 g ha-1, (trifloxysulfuron + ametryn, 27,75 + 1097,3 g ha-1 mais (diuron + hexazinone, 702 + 198 g ha-1, metribuzin (960 g ha-1 mais (trifloxysulfuron + ametryn, 27,75 + 1.097,3 g ha-1 e metribuzin (960 g ha-1 mais (diuron+ hexazinone, 702 + 198 g ha-1] e duas testemunhas sem aplicação: uma mantida infestada e outra capinada. Apesar das injúrias visuais ocasionadas pelos herbicidas, isso não refletiu negativamente no número de colmos viáveis por metro e no diâmetro de colmos de cana. Contudo, as plantas tratadas com diuron + hexazinone apresentaram menor altura de colmos. O tratamento diuron + hexazinone, isolado ou em mistura com trifloxysulfuron + ametryn, foi eficaz no controle de todas as espécies de corda-de-viola. A aplicação isolada de trifloxysulfuron + ametryn controlou satisfatoriamente apenas I. hederifolia. O metribuzin foi eficaz no controle de I. grandifolia, I. quamoclit, M.aegyptia e M. cissoides. Além destas, a associação desse herbicida com diuron + hexazinone resultou em excelente controle de I. hederifolia. A mistura de metribuzin com trifloxysulfuron + ametryn foi eficaz para I. hederifolia, M. aegyptia e M. cissoides. Os novos fluxos de emergência de corda-de-viola constatados após a aplicação dos herbicidas ou eliminação manual das plantas nas parcelas (na testemunha capinada não prejudicariam o corte mecanizado

  9. 长沙市居民食用甘薯情况与健康状况的调查%Survey on Eating Ipomoea batatas and Health of Residents in Changsha City

    Institute of Scientific and Technical Information of China (English)

    张音; 夏延斌; 张娟; 熊学斌; 邓传波; 李利晓; 钟燕青; 孟潇; 常怡

    2011-01-01

    目的:全面深入地研究长沙市居民食用甘薯与生活观念、健康状况之间的关系。方法:随机抽取长沙市不同地区年龄在20岁以上的居民发放调查问卷进行调查。结果:在405例调查者中,18.27%的人经常食用甘薯,61.98%的人偶尔食用甘薯,19.76%的人从不吃甘薯;甘薯被视为营养全面而均衡的保健食品,受到青年一代的青睐,他们不食用甘薯的比例在逐步降低;老年人群食用甘薯的频率较高,常食甘薯对于心血管疾病、糖尿病和骨质疏松症可能有一定的预防作用;经常食用甘薯的老年人食用粗粮、水果,参加锻炼和娱乐的比例较高,食用保健品的比例较低,健康营养意识比较强。%【Objective】In order to research the relationship among eating Ipomoea batatas,life notion and health of residents in Changsha.【Method】Residents of all more than 20 years old in different areas in Changsha were randomly chosen to answer the questionnaires.【Result】In 405 participants,18.27% of them often ate Ipomoea batatas,61.98% occasionally consumed them,and 19.76% never ate them.Ipomoea batatas which were seen as comprehensive and balanced nutrition health food were favoured by the younger generation.The proportion of the younger generation who did not eat Ipomoea batatas reduced gradually.Older people had a higher frequency of eating Ipomoea batatas,which might have a protective effect on cardiovascular disease,diabetes,and osteoporosis.Old people who often ate Ipomoea batatas also had a higher percentage of eating whole grains and fruits,doing exercise and entertainment,and they had a low proportion of eating health products.Health and nutrition consciousness of these people was stronger.

  10. Ipomoea batatas (Convolvulaceae)-mediated synthesis of silver nanoparticles for controlling mosquito vectors of Aedes albopictus, Anopheles stephensi, and Culex quinquefasciatus (Diptera:Culicidae).

    Science.gov (United States)

    Pavithra Bharathi, V; Ragavendran, C; Murugan, N; Natarajan, D

    2016-12-08

    We proposed an effective and eco-friendly control of dengue, malaria, and filariasis-causing vectors. We tested Ipomoea batatas leaves-mediated silver nanoparticles (AgNPs) against first to fourth instar larvae and adults of Aedes albopictus, Anopheles stephensi, and Culex quinquefasciatus at different concentrations. The synthesized AgNPs showed broad spectrum of larvicidal and adulticidal effects after 48 h of exposure. The characterization of synthesized AgNPs was done using various spectral and microscopy analyses. The maximum efficacy was observed in synthesized AgNPs against the adult of Ae. albopictus with the LC50 and LC90 values were 10.069 and 15.657 μg/mL, respectively, followed by others.

  11. Predation by Megacerus baeri (Pic, 1934 (Coleoptera: Bruchidae on seeds of Ipomoea imperati (Convolvulaceae at Joaquina beach, Florianópolis, southern Brazil

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    Helena Picolli Romanowski

    2005-05-01

    Full Text Available Bruchidae beetles are important seed predators that feed on the seeds of a large array of plants. Predation levels by the bruchid Megaceus baeri were quantified on 333 fruits of beach morning glory Ipomoea imperati (Convolvulaceae, collected at Joaquina Beach, Santa Catarina Island (27o36’S; 48o 27’W, Santa Catarina State, Brazil. The damage caused by M. baeri larva had affected 73% of the fruit collected and had killed 67% of the seeds. Among the fruits that had been predated, 76.5% had only one seed and 23.5% had two seeds. The average size of the seeds attacked by M. baeri was larger than the average size of those that had not been predated. Since this bruchid has to complete its development in a single seed, it is suggested that seed size reflects its quality as a resource.

  12. 巴西甘薯叶亲脂性成分研究%Lipophilic constituents from the leaves of Ipomoea batatas(cv. Simon)

    Institute of Scientific and Technical Information of China (English)

    罗建光; 孔令义

    2005-01-01

    巴西甘薯叶[Ipomoea batatas Lam.(cv.Simon)]的氯仿成分经反复硅胶柱层析分离得到了8个化合物,通过理化性质和波谱方法分别鉴定为:乙酰-β-香树醇(1)、木栓酮(2)、表木栓醇(3)、三十烷醇(4)、β-谷甾醇(5)、咖啡酸乙酯(6)、东莨菪素(7)和胡萝卜苷(8).其中3、4、6、7和8为首次从该植物中分得.

  13. 甘薯的主要营养成分和保健作用%Main Nutritional Components of Ipomoea Batatas and Its Healthy Functions

    Institute of Scientific and Technical Information of China (English)

    张立明; 王庆美; 王荫墀

    2003-01-01

    @@ 甘薯(Ipomoea batatas)是世界上重要的粮食、饲料和工业原料作物,普遍种植于世界上热带和亚热带地区的100多个国家.甘薯自400多年前引入中国,目前我国已成为世界上最大的甘薯生产国,据FAO(2000年)统计,我国甘薯年种植面积616万hm2,总产11.7亿t,分别占世界种植面积和总产的68.1%和86.8%.

  14. 巴西甘薯叶黄酮类成分的研究%Study on flavonoids from leaf of Ipomoea batatas

    Institute of Scientific and Technical Information of China (English)

    罗建光; 孔令义

    2005-01-01

    目的:研究巴西甘薯叶Ipomoea batatas的黄酮类成分.方法:乙醇提取后分别以氯仿、醋酸乙酯和正丁醇萃取,醋酸乙酯部位用硅胶柱色谱,Sephadex LH-20凝胶柱色谱等色谱法分离;通过理化性质和波谱方法鉴定结构.结果:分离并鉴定了5个黄酮类化合物:银椴苷(tiliroside)(Ⅰ),紫云英苷(astragalin)(Ⅱ),鼠李柠檬素(rhamnocitrin)(Ⅲ),鼠李素(rhamnetin)(Ⅳ),山柰酚(Ⅴ).结论:上述黄酮类成分均首次从该植物中分得.

  15. Efeito do glutaraldeído na adsorção de extrato proteico da Ipomoea Batatas (L Lam na superficie de uma zeólita analcima Effect of the glutaraldeide on the adsorption of proteic extract of the Ipomoea Batatas (L Lam in analcime zeolite surface

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    A. C. S. Gondim

    2005-09-01

    Full Text Available A zeólita analcime (Na16Al16Si32O96.nH 2O foi submetida a ataque ácido com HCl 0,25 mol.L-1, seguido da funcionalização com glutaraldeído, foi imersa em extrato protéico obtido da polpa da batata-doce (Ipomoea Batatas (L Lam, que tem em sua composição a enzima polifenol oxidase (PFO. As amostras foram submetidas a procedimentos de caracterização por microscopia eletrônica de varredura, espectroscopia na região do infravermelho com transformada de Fourier e termogravimetria.The analcime zeolite (Na16Al16Si32O96.nH2O was treated with hydrochloric acid 0.25 mol.L-1, followed by the funcionalization with glutaraldehyde and immersed in the proteic extract obtained from gross extract of sweet potato pulp (Ipomoea Batatas (L Lam, which presents in its composition the polyphenol oxidase enzyme (PPO. Other samples were submitted to the same procedure with characterizations by scanning electron microscopy, Fourier Transform Infrared spectroscopy and thermogravimetry.

  16. EFEK HIPOGLIKEMIK EKSTRAK ETANOL UMBI KETELA RAMBAT (Ipomoea batatas P (EEUKR PADA MENCIT SWISS YANG DIINDUKSI ALOKSAN

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    Akrom Akrom

    2014-05-01

    Full Text Available Sweet potatos rood contain lots of beta-carotene, polyphenols and flavonoids. Alloxan proven pancreatic cell damage through oxidative stress mechanisms. The compound beta-carotene, polyphenols and flavonoids are thought to antioxidative and cytoprotective, inhibit cell damage caused by alloxan exposure. This study aimed to determine the effect of ethanolic extract of sweet potatos (Ipomoea batatas P (EEUKR on blood glucose levels and pancreatic histopathology on alloxaninduced Swiss mice. Used 15 test animals, Swiss mice, with an average weight of 20-30 grams. Test animals were divided into 5 groups, with each group consisting of 3 mice. Group I was the negative control group who were given distilled water (akua group, and group II, III, IV and V are the treatment group were given ethanolic extract of sweet potatos rood (EEUKR at a dose of 2.5, 7.5, 22.5 and 67.5 mg/KgBW/day orally for 10 days, 7 days before and 3 days after the alloxan induced. Alloxan induction performed on the 7th day intraperitoneally at a dose of 120 mg/kgBW. Examination of blood glucose levels conducted on 4th and day 10th day of treatment. On the 10th day of the test animals were sacrificed for isolated pancreas and histopathologic examination. Analysis of variance conducted to determine the significance difference in average blood glucose levels between groups during the test and ANOVA followed by LSD test at 95% confidence level. Pancreatic histopathology data were analyzed qualitatively. The results showed that the EEUKR dose of 22.5 and 67.5 mg/kgBW/day had the effect of hypoglycemia in Swiss mice. Blood glucose levels of Swiss mice before alloxan induced in treatment group with EEUKR dose of 67,5 mg/Kg BW were lower than blood glucose levels of akua groups, statistically significant (p <0,05. Blood glucose levels of alloxan induced swiss mice of treatment groups with EEUKR dose of 22.5 and 67.5 mg/KgBW were lower than blood glucose level of akua group and

  17. ipomoea batatas (l

    African Journals Online (AJOL)

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    K Sa. 7.98 %. Kt. 6.44 %. K fixation h-1 contact time. -4.89 mg/100 g soil. K fixation 2400 h .... Effect of moisture level (60% WHC every day) and fertilizer on stock diameter. T2. T4. T6. 0. 10. 20. 30. 40. 50. 60. 70. 80 .... within 15 days where as in T2, T4 and T6 34–44% .... Follet, R.M., Murphy., L.S. and Donahue, R.L. (1981).

  18. Phenology and morphological diversity of sweet potato (Ipomoea batatas landraces of the Vale do Ribeira Fenologia e diversidade morfológica de etnovariedades de batata-doce (Ipomoea batatas do Vale do Ribeira

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    Elizabeth Ann Veasey

    2007-08-01

    Full Text Available The phenotypic diversity of sweet potato (Ipomoea batatas landraces was assessed using morphological traits, verifying how this diversity is distributed among the households and settlements of the Vale do Ribeira, Brazil. A total of 74 accessions, involving 53 landraces, collected from 30 households distributed among 18 settlements that practice traditional agriculture in the municipalities of Iguape, Ilha Comprida, and Cananeia, as well as four commercial varieties acquired in markets of Iguape and Piracicaba, were evaluated under an ex situ experimental condition in Piracicaba, SP, Brazil. Nine phenological and floral descriptors, nine morphological vegetative aerial descriptors and five storage root traits were recorded. The 14 aerial vegetative and root descriptors were evaluated as binary data, totaling 74 attributes. Cluster analyses were made using the Jaccard similarity index and the UPGMA (unweighted pair group method with arithmetic mean agglomerative method. Binary data was also submitted to a variance analysis (AMOVA. No defined groups were observed, indicating that the diversity of the landraces is not structured in space, but considerable morphological variation was found in this area (Jaccard similarity index varying from 0.12 to 1.0. Most of the variability occurred within households (64.4%, followed by the distribution among households within settlements (27.1% and among settlements (8.4%. Thus, the traditional agriculturists of Vale do Ribeira maintain a high morphological diversity for sweet potato within their households, which can be assumed to be produced by the outcrossing mating system of this species and somatic mutation events, as well as the exchange system at local and regional levels.Avaliou-se a diversidade fenotípica de etnovariedades de batata-doce através de descritores morfológicos, visando verificar como esta diversidade está distribuída em nível de roças e comunidades do Vale do Ribeira, SP, Brasil

  19. Evaluación de la dinámica del crecimiento in vitro en callos de Ipomoea batatas

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    Orlando S. González Paneque

    2011-08-01

    Full Text Available Evaluation of the dynamics of the growth in vitro callus of Ipomoea batatas ResumenEl cultivo del boniato presenta una gran importancia, ya que se puede emplear en la alimentación humana y animal, así como en la industria; el mismo produce raíces reservantes de gran valor calórico y nutritivo con alto contenido de carbohidratos. Entre las raíces y tubérculos cultivados es el segundo en importancia y representa más del 80% de la producción mundial. El empleo de las técnicas in vitro constituye una poderosa herramienta en la explotación comercial, propiciando el empleo de la micropropagación en diferentes especies. Para desarrollar el presente trabajo se recolectaron raíces tuberosas pertenecientes al clon Inivit B 93-1. Se procedió a la formación de callos potencialmente embriogénicos, para lo cual se emplearon explantes de limbos foliares, desinfectados con hipoclorito de sodio (1% y sembrados en el medio de cultivo propuesto por Murashige y Skoog (1962, vitaminas MS (10,0 ml/l-1, mioinositol (100 mg/l-1, sacarosa (3%, gelrite (0,2%, 2,4-D (0,25-2,5 mg/l-1 y 6-BAP (0,25-1,0 mg/l-1, el pH fue ajustado a 5,8 ± 0,01 mantenidos en la oscuridad durante treinta días, lográndose los mejores resultados con el uso del 2,4-D (0,50 mg/l-1 y 6-BAP (0,25 mg/l-1, y en los mismos se evaluó la dinámica del crecimiento y se lograron los mejores resultados entre los 28 y 32 días después de la siembra, para lo cual los resultados obtenidos servirán de base a otros estudios y permitirán evaluar, controlar y desarrollar estrategias para la conservación y el uso de los recursos naturales, dando cumplimiento al objetivo referente a estudiar la dinámica del crecimiento en la formación de callos potencialmente embriogénicos en el cultivo del boniato. Palabras clave: boniato, cultivo in vitro, reguladores del crecimiento. Abstract The cultivation of the sweet potato presents a great importance, since you can use in the human feeding

  20. 美国紫薯试管苗微型繁殖的研究%Study on Micro Propagation of Tube Seedlings of the Ipomoea batatas

    Institute of Scientific and Technical Information of China (English)

    2013-01-01

    In order to meet the large needs for tube seedlings of Ipomoea batatas after heat treatment ,with the method of tissue culture and micro cutting ,the mini tender stem segments were used as material to do the re‐search on micro propagation of tube seedlings in vitro .The results demonstrated that in the media of 1/2MS+IAA 0 .2 mg・L‐1 +Sucrose 15 g・L‐1 ,one tube seedling ,which reproduced by mini tender stem segments with 2 growing points and 2 blades in vitro ,could breed more than two million tube seedlings each year ;with 2 points and 2 blades of micro cuttings to the seedbed propagation speed was increased by 1 .8 times .Colonization of the , Ipomoea batatas 48 .9% .%  为满足人们对经过热处理的美国紫薯试管苗种苗的大量需求,以微型嫩茎段为材料,采用组织培养和微型扦插的方法,进行了试管苗微型繁殖的研究。结果表明:在1/2MS+IAA0.2 mg・L‐1+蔗糖15 g・L‐1培养基上,以具有2个生长点和2个叶片的微型茎段繁殖试管苗,1株试管苗每年能繁殖200多万株试管苗;以具有2个生长点和2个叶片的苗微型茎段扦插到苗床上,其繁殖速度提高了1.8倍。定植的微型扦插试管苗生长较旺盛,美国紫薯的产量提高48.9%。

  1. Study on The Content of Pb in Twigs And Leaves of Kangkung (Ipomoea reptans Poir Boiled With The Addition of NaCl And Acetic Acid

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    Poppy Hartatie Hardjo

    2004-12-01

    Full Text Available Kangkung is a kind of favorable vegetables that used to grow near a river, and is cultivated and watered with water from the river. If the river is polluted by heavy metals, there is a risk that the plant is contaminated too. A study on the content of Pb in kangkung planted in Pb contaminated media has been conducted, and it was proven that Pb was found in the plant. Land kangkung (Ipomoea reptans was used as sample, and was planted in hydrophonic media, and watered with Multigrow Complete Plant Food (2000 mg/L and Pb solution (2 mg/L twice a day. Samples were taken based on the age of 54 days, then the twigs and leaves were boiled in different ways: I. Boiled with no addition, II. Boiled with addition of NaCl , and III. Boiled with addition of acetic acid. IV. Unboiled sample as the control. Inductively Coupled Plasma Spectrometer (ICPS Fison 3410+ was used to measure the Pb content. It was shown that boiling the kangkung reduced the Pb content in the leaves as well as in the twigs; however, the acetic acid addition showed the least effect. In the leaves the three different ways of boiling did not show significant different, while in twigs the different was significant.

  2. Evaluación agronómica de diez clones promisorios CIP y dos materiales nativos de Ipomoea batatas L.

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    Tique José

    2009-08-01

    Full Text Available

    Con el propósito de rescatar la riqueza genética de la batata (Ipomoea batatas, durante dos ciclos de cultivo se realizaron evaluaciones agronómicas de dos materiales nativos, en comparación con diez clones provenientes del Centro Internacional de la Papa, clasificados allí como promisorios. El trabajo se llevó a cabo en el municipio de Coyaima, en el departamento del Tolima (Colombia, con la participación de los integrantes del Cabildo Indígena Autónomo Independiente Buenavista, y la colaboración del Consorcio Latinoamericano y del Caribe de Apoyo a la Investigación y el Desarrollo de la Yuca. Los materiales nativos se colectaron en parcelas de agricultores y se clasificaron como ‘Bonanza’ y ‘Sangretoro’, nombres tradicionales dados por los indígenas de la zona. El experimento fue desarrollado a través de un diseño de bloques completos al azar, con siete tratamientos y cuatro repeticiones. Los tratamientos correspondieron a los cinco clones CIP y las dos variedades regionales. Los resultados de la investigación permitieron cuantificar el alto potencial productivo de la batata como especie cultivada, encontrándose materiales muy promisorios que pueden ser incorporados a modelos agropecuarios de producción.

  3. Bioethanol production from sweet potato (Ipomoea batatas L. flour using co-culture of Trichoderma sp. and Saccharomyces cerevisiae in solid-state fermentation

    Directory of Open Access Journals (Sweden)

    Manas Ranjan Swain

    2013-04-01

    Full Text Available The aim of this work was to study the optimiation of co-culturing of Trichoderma sp. and Saccharomyces cerevisiae (1:4 ratio on sweet potato (Ipomoea batatas L. flour (SPF for the production of bio-ethanol in solid-state fermentation (SSF. Maximum ethanol (172 g/kg substrate was produced in a medium containing 80% moisture, ammonium sulphate 0.2%, pH 5.0, inoculuted with 10% inoculum size and fermented at 30ºC for 72h. .Concomitant with highest ethanol concentration, maximum ethanol productivity (2.8 g/kg substrate/h, microbial biomass (23×10(8 CFU/ g substrate, ethanol yield (47 g/100g sugar consumed and fermentation efficiency (72% were also obtained under these conditions. Cell interaction was observed familiar between the viable cells of Trichoderma sp. and S. cerevisiae when co-cultured. Ethanol production ability by the co-culture was 65 % higher than the single culture of S. cerevisiae from un-saccharified SPF.

  4. Synergistic Effect of Compounds from a Chinese Herb: Compatibility and Dose Optimization of Compounds from N-Butanol Extract of Ipomoea stolonifera

    Science.gov (United States)

    Cai, Congyi; Chen, Yicun; Zhong, Shuping; Zhang, Yanmei; Jiang, Jiyang; Xu, Han; Shi, Ganggang

    2016-01-01

    The herbal medicine Ipomoea stolonifera (I. stolonifera) has previously been shown to have considerable anti-inflammatory potential in vivo and in vitro. To establish a method for exploring the synergistic effects of multiple compounds, we study the compatibility and dose optimization of compounds isolated from n-butanol extract of I. stolonifera (BE-IS). Raw264.7 cell was treated with lipopolysaccharide (LPS) in the presence of compounds from BE-IS, namely scopoletin, umbelliferone, esculetin, hesperetin and curcumin, using the orthogonal design, uniform design and median-effect method. To verify the best efficacy of principal constituents in vivo, the uniform design was used in the croton oil-induced mouse ear edema model. The results from LPS-induced the production of prostaglandin E2 (PGE2) show that, esculetin, curcumin and hesperetin were the principal constituents that had synergistic effects when used at the optimal ratio. Additionally, the principal constituents were found to work synergistically in the croton oil-induced mouse ear edema model at low doses. It turned out that the three experimental optimization and analysis methods (orthogonal design, uniform design and median-effect method) can be effectively used to solve both compatibility and dose optimization for combined use of multiple compounds. PMID:27255791

  5. The geographic mosaic of herbicide resistance evolution in the common morning glory, Ipomoea purpurea: Evidence for resistance hotspots and low genetic differentiation across the landscape.

    Science.gov (United States)

    Kuester, Adam; Chang, Shu-Mei; Baucom, Regina S

    2015-09-01

    Strong human-mediated selection via herbicide application in agroecosystems has repeatedly led to the evolution of resistance in weedy plants. Although resistance can occur among separate populations of a species across the landscape, the spatial scale of resistance in many weeds is often left unexamined. We assessed the potential that resistance to the herbicide glyphosate in the agricultural weed Ipomoea purpurea has evolved independently multiple times across its North American range. We examined both adaptive and neutral genetic variations in 44 populations of I. purpurea by pairing a replicated dose-response greenhouse experiment with SSR genotyping of experimental individuals. We uncovered a mosaic pattern of resistance across the landscape, with some populations exhibiting high-survival postherbicide and other populations showing high death. SSR genotyping revealed little evidence of isolation by distance and very little neutral genetic structure associated with geography. An approximate Bayesian computation (ABC) analysis uncovered evidence for migration and admixture among populations before the widespread use of glyphosate rather than the very recent contemporary gene flow. The pattern of adaptive and neutral genetic variations indicates that resistance in this mixed-mating weed species appears to have evolved in independent hotspots rather than through transmission of resistance alleles across the landscape.

  6. Evaluación de la calidad del trabajo de aperos de labranza en la labor de surcado para el cultivo del boniato (Ipomoea batatas (Lam Poir

    Directory of Open Access Journals (Sweden)

    Alfonso Enrique Ortiz Rodríguez

    2011-01-01

    Full Text Available La investigación se desarrolló en la Unidad Básica de Producción Cooperativa (UBPC "El Palmar" perteneciente a la Empresa de Cultivos Varios "Paquito Rosales Benítez" de Veguitas, municipio Yara, provincia de Granma, en el período de diciembre de 2005 a enero de 2008 (tres campañas; con el objetivo de evaluar en la labor de surcado aperos de diferentes características constructivas para el cultivo del boniato (Ipomoea batatas (Lam Poir en un Fluvisol, teniendo en cuenta el comportamiento de los índices de calidad de la labor. El Surcador Fertilizador IIMA-BALDAN 4 500 obtuvo el mejor resultado de las dimensiones del cantero con 98,3% y 100% de calidad en los valores del plato, ancho y altura, según las exigencias agrotécnicas del cultivo referidas por el Instituto de Investigaciones de Viandas Tropicales.

  7. Intake, growth and carcass yield of indigenous goats fed market wastes of sweet potato (Ipomoea batatas) vines and scarlet eggplant (Solanum aethiopicum).

    Science.gov (United States)

    Katongole, C B; Bareeba, F B; Sabiiti, E N; Ledin, I

    2009-12-01

    Intake, growth and carcass yield of indigenous goats fed basal diets of market wastes of sweet potato (Ipomoea batatas) vines (SPV) and scarlet eggplant (Solanum aethiopicum) (SEP) were examined in two experiments. In experiment I, the effect of presentation method (chopping, hanging or adding molasses) on the intake of SEP and SPV was evaluated. Presentation method did not influence the intake of SPV while hanging resulted in the highest (P goats were supplemented with a concentrate (25% cottonseed cake +75% maize bran) in four dietary groups: SEPD, SPVD, SEP+SPV and Control where elephant grass (Pennisetum purpureum) was offered as the roughage. SEP were offered by hanging, while SPV were chopped. The goats were slaughtered after 12 weeks of feeding. Goats in the SEPD group constantly lost weight, hence were not followed to the end. Average daily gain and hot carcass weight were highest (P goat meat production where standard feed resources are scarce when fed with an energy-protein concentrate. SEP are poorly consumed by goats, hence cannot be used as their sole basal feed.

  8. 紫红薯膳食纤维漂白工艺研究%Studies on the Bleaching Technology of Purple Ipomoea batatas Dietary Fiber

    Institute of Scientific and Technical Information of China (English)

    吴少福; 蒋艳; 上官新晨; 沈勇根; 徐嘉琪; 罗娟

    2011-01-01

    为了得到色泽较浅的紫红薯膳食纤维,以样品的白度为指标,采用单因素和正交优化试验对H2O2漂白紫红薯膳食纤维的工艺进行研究.结果表明:将紫红薯膳食纤维用40 g/L H2O2溶液按1:15调成浆,pH值调整为10.0,在60 ℃的水浴中保温处理180 min,样品的白度值达到85.39%.%In order to obtain lighter -color purple lpomoea batatas dietary fiber, taking whiteness of the sample as an indicator, the bleaching technology of the purple lpomoea batatas dietary fibers was researched by H202 method. The optimum technologic parameters of the purple Ipomoea batatas IDF bleaching was obtained with single -factor and orthogonal experiment. The results showed that: 1:15 proportion of purple lpomoea batatas IDF to H2O2 solution, the concentration of hydrogen peroxide was 40 g/L, pH 10, temperature 60 ℃, time 180 min. Its brightness reached 85.39%.

  9. Identification of the glycosidase inhibitors swainsonine and calystegine B2 in Weir vine (Ipomoea sp. Q6 [aff. calobra]) and correlation with toxicity.

    Science.gov (United States)

    Molyneux, R J; McKenzie, R A; O'Sullivan, B M; Elbein, A D

    1995-06-01

    The polyhydroxy alkaloid glycosidase inhibitors swainsonine [1] and calystegine B2 [6] have been identified as constituents of the seeds of the Australian plant Ipomoea sp. Q6 [aff. calobra] (Weir vine) by gas chromatography-mass spectrometry and by their biological activity as inhibitors of specific glycosidases. This plant, which is known only from a small area of southern Queensland, has been reported to produce a neurological disorder when consumed by livestock. The extract of the seeds showed inhibition of alpha-mannosidase, beta-glucosidase, and alpha-galactosidase, consistent with the presence of 1 and alkaloids of the calystegine class. Histological examination of brain tissue from field cases of sheep and cattle poisoned by Weir vine showed lesions similar to those observed in animals poisoned by the swainsonine-containing poison peas (Swainsona spp.) of Australia and locoweeds (Astragalus and Oxytropis spp.) of North America. These results indicate that Weir vine poisoning is an additional manifestation of the induced lysosomal storage disease, mannosidosis, possibly exacerbated by inhibition of the enzymes beta-glucosidase and alpha-galactosidase by calystegine B2. This is the first reported example of a single plant species capable of producing structurally distinct glycosidase inhibitors, namely, alkaloids of the indolizidine and nortropane classes.

  10. Protective role of Ipomoea obscura (L.) on cyclophosphamide-induced uro- and nephrotoxicities by modulating antioxidant status and pro-inflammatory cytokine levels.

    Science.gov (United States)

    Hamsa, T P; Kuttan, Girija

    2011-06-01

    Cyclophosphamide (CP) is widely used, alone or in combination with other chemotherapeutic agents, for the treatment of neoplastic diseases. Its urotoxicity may cause dose-limiting side-effects, for example haemorrhagic cystitis. This urotoxicity may lead to nephrotoxicity. In the present study, we investigated the protective role of Ipomoea obscura against CP-induced uro- and nephrotoxicities in animal models. Swiss albino mice were treated with an acute dose of CP (1.5 mmol/kg body wt ip) in the presence and absence of alcoholic extract of I. obscura (10 mg/kg, body wt, ip) for 5 days. The toxicities caused by CP were reversed by the extract administration as evident from the decrease in BUN, serum creatinine levels as well as an increase in body weight. A significant increase in kidney antioxidant system such as, GSH, SOD, CAT, and GPx was also observed in extract-treated animals. Histopathological analysis of urinary bladder and kidney indicated that CP-induced tissue damage was significantly reduced in animals treated with I. obscura. The lowered levels of cytokines IFN-γ and IL-2, after CP treatment were found to be increased in treated animals. At the same time the level of proinflammatory cytokine TNF-α, which was elevated during CP administration, was significantly reduced by extract administration. This study clearly demonstrates that I. obscura can ameliorate CP-induced bladder and renal toxicities by modulating antioxidant status and proinflammatory cytokine levels.

  11. Remediation of textile azo dye acid red 114 by hairy roots of Ipomoea carnea Jacq. and assessment of degraded dye toxicity with human keratinocyte cell line.

    Science.gov (United States)

    Jha, Pamela; Jobby, Renitta; Desai, N S

    2016-07-05

    Bioremediation has proven to be the most desirable and cost effective method to counter textile dye pollution. Hairy roots (HRs) of Ipomoea carnea J. were tested for decolourization of 25 textile azo dyes, out of which >90% decolourization was observed in 15 dyes. A diazo dye, Acid Red 114 was decolourized to >98% and hence, was chosen as the model dye. A significant increase in the activities of oxidoreductive enzymes was observed during decolourization of AR114. The phytodegradation of AR114 was confirmed by HPLC, UV-vis and FTIR spectroscopy. The possible metabolites were identified by GCMS as 4- aminobenzene sulfonic acid 2-methylaniline and 4- aminophenyl 4-ethyl benzene sulfonate and a probable pathway for the biodegradation of AR114 has been proposed. The nontoxic nature of the metabolites and toxicity of AR114 was confirmed by cytotoxicity tests on human keratinocyte cell line (HaCaT). When HaCaT cells were treated separately with 150 μg mL(-1) of AR114 and metabolites, MTT assay showed 50% and ≈100% viability respectively. Furthermore, flow cytometry data showed that, as compared to control, the cells in G2-M and death phase increased by 2.4 and 3.6 folds respectively on treatment with AR114 but remained unaltered in cells treated with metabolites.

  12. Simultaneous determination of four phenolic compounds in extracts of aerial parts of Ipomoea pes-caprae (L. R. Br. (Convolvulaceae by HPLC-UV

    Directory of Open Access Journals (Sweden)

    Daniela Maes Dutra

    2014-01-01

    Full Text Available Among other applications, Ipomoea pes-caprae is popularly used to treat jellyfish stings, supporting the development of a product for dermatological use. Hydroethanolic spray-dried extract was chosen for the further development of phytomedicines, and a stability-indicative HPLC-UV method was developed and validated for the determination of isoquercitrin and isochlorogenic acids A, B and C. The method was developed using a C18 column (250 x 4.6 mm, 5 µm with an acetonitrile:water mobile phase at pH 3.0 in a gradient run. The four constituents and other unidentified components of the extract were appropriately resolved without interference of degradation products after stress tests (acid, alkali, neutral, oxidant, photolysis. The method showed linearity in the isoquercitrin concentration range from 5.0-50.0 µg mL-1, with adequate precision (RSD% < 2.5% for the intra- and inter-day studies, accuracy (recovery of 100.0 ± 2.0%, and robustness. Both the herbal drug and spray-dried extract of I. pes-caprae were subjected to stability studies in accelerated and long-term conditions over four months. The samples maintained their characteristics and marker contents (< 10% of variation.

  13. 成熟度与采收部位对川泽泻种子质量的影响研究%Effects of Maturity and Collected Part on Seed Quality of Alisma Plantago-aquatica

    Institute of Scientific and Technical Information of China (English)

    陈琪; 陈兴福; 杨文钰; 张宝林; 张玉; 杨俊东

    2013-01-01

    采集川泽泻不同成熟度的种子和两个批次的不同采收部位的种子,对其果实和种子外观质量、种子净度、含水量、千粒重和发芽率等进行比较研究,为川泽泻优质种子的生产提供参考.结果表明,川泽泻果实成熟过程中果实和种子外观形态及重量变化明显且较一致;不同成熟度的新鲜和阴干种子发芽率均明显不同,且低成熟度种子宜用于储藏,高成熟度种子宜用于即播.不同采收部位的种子中主花茎第三轮及以上部位(中上部)种子质量高于其他部位.种子培育过程中应尽量控制植株生长一致并及时摘除主花茎第一轮短枝和第二轮花序;种子采集宜分批进行,主花茎底部花序应提前采收,中上部花序后期采收,均以果实大量成熟时(呈褐色)为宜.%In order to provide a potential reference for collecting high quality seeds of A. plantago-aquatica, the seeds which collected from different grades of maturity and different collected parts in two batches were compared according to the appearance quality of fruits and seeds, the purification, moisture content, weights per thousand seeds and germination rate of the seeds. The results showed that the variation of appearance and weigh on both the fruits and seeds were obvious and overall consistent as the the fruits gradually ripened. They were markedly varied in germination rate of both the fresh seeds and the seeds dried in the shade in different grades of maturity. The seeds of low grade of maturity were suited to be store while the seeds of high degree of maturity should be sown immediately. The quality of seeds from the third and the higher round of the scape (the top and middle of the scape) were better than the others. During the process of cultivation, it was supposed to take steps to regulate the plants to growing in the same level and promptly wipe off the short inflorescence in the first round branch and the second round branch of scape

  14. Hyperspectral Estimation for Chlorophyll Contents in the Leaves of Lpomoea aquatica F. Under Cadmium Stress%镉胁迫下空心菜叶绿素质量分数的高光谱估算

    Institute of Scientific and Technical Information of China (English)

    顾艳文; 李帅; 高伟; 魏虹

    2015-01-01

    In order to rapidly and non‐destructively monitor heavy metal contamination of the leafy vegeta‐ble L pomoea aquatica F. ,a potted experiment was conducted .Six different Cd concentrations treatments (CK ,T1 ,T2 ,T3 ,T4 and T5) were established with 3 replicates per treatment ,where CK ,T1 ,T2 ,T3 , T4 and T5 represented 0 ,0.5 ,1 ,5 ,10 and 20 Cd mg/kg dry soil .The hyperspectral reflective rate of fif‐ty‐four samples was scanned by ASD FieldSpec 3 HR and their chlorophyll concentrations were measured using Shimadzu UV2550 spectrophotometer on the15th ,30th and 45th days after the beginning of treat‐ments .Further ,the correlation between spectral data and chlorophyll contents was analyzed ,and the fit‐ting models for chlorophyll contents were established using the sensitive spectrum and characteristic pa‐rameters .The results showed that total chlorophyll content decreased gradually with higher Cd concentra‐tion and longer treatment time .The differences of leaf reflectance among different treatments were signifi‐cant at green peak and infrared wavelength .In the wave range of 350 -1 300 nm ,the leaf reflectance showed a similar trend between the 45d and other treatments .However ,the difference among the plant leaf reflectance under different Cd concentrations was more significant in the 45‐d treatment ,as compared to that in 15‐d and 30‐d treatments .In peak green and red edge range of wavelengths ,the hyperspectral re‐flectance was well correlated with chlorophyll contents (p<0.01) ,and R2 of R540 and R710 with chloro‐phyll content was 0.753 and 0.719 respectively (p <0.01) after data re‐sampling .Therefore ,R540 and R710 were extracted as the sensitive bands to construct estimation models .The linear ,logarithmic ,in‐verse and parabola fitting models between R540 ,R710 ,G PR and SG P and chlorophyll contents had bet‐ter sensitivity compared with other spectral parameters ,and the calibration R2 and verification R2 were ap

  15. Use of planted biofilters in integrated recirculating aquaculture-hydroponics systems in the Mekong Delta, Vietnam

    DEFF Research Database (Denmark)

    Trang, N.T.D.; Brix, Hans

    2014-01-01

    and 2842 g fresh aboveground biomass of Ipomoea aquatica Forssk., Lactuca sativa L. and C. glauca, respectively, were produced. The leafy vegetables provide some extra income besides fish products, whereas C. glauca provides nice flowers and contributes to a significant nutrient removal with annual uptake...

  16. Identification de quelques contraintes à la production de manioc Manihot utilissima, de patate douce Ipomoea batatas et de pomme de terre Solanum tuberosum à l'Est du Zaïre

    Directory of Open Access Journals (Sweden)

    Ngoy Kadiebwe, N.

    1994-01-01

    Full Text Available Identification Of Some Constraints To The Production Of Tubers : Manihot utilissima, Ipomoea batatas and Solanum tuberosum In Eastern Zaire. An agricultural enquiry held to tubercular plants producers in radius of about 20 km to the Research Center has allowed to reveal some contraints related to the production of these speculations in order to pose certain investigations. The main contraints among them are utilization of too long cycle varieties with insufficient quality and quantity, ignorance of the selection production and conservation techniques without forgetting the difficultes of evacuating the production.

  17. The Research on High Yield Cultivation Technology of the High Starch Ipomoea Batatas Lam Based on Orthogonal Experiment%基于正交实验的高淀粉甘薯高产栽培技术研究

    Institute of Scientific and Technical Information of China (English)

    尤瑞玲; 李红忠; 史本林

    2012-01-01

    The ipomoea batatas lam is one of world staple food crops, also is the important industrial crop and the feed crops. In recent years, the domestic scholars have conducted the massive research to the high yield cultivation technique of the ipomoea batatas lam. In order to improve the yield of 'Shangshu 19', the article uses the orthogonal experiment method through combining the experiment, basing on the cultivation technology of ipomoea batatas lam in different transplanting time, planting density, ridge width, ridge height four factors. The article conducts the contrast experimental study for the ipomoea batatas lam, in order to select the best comprehensive technique measures and guide the field production. The results showed that, the five combinations got the highest yield. There were significant differences with other groups. The important cultivation and management measures were the timely plug, the proper ridge and the rational application of fertilizer.%甘薯是世界主要粮食作物之一,也是重要的工业原料作物和饲料作物.近年来,中国学者对甘薯高产栽培技术进行了大量研究.为了提高能源型‘商薯19’的产量,采用正交实验方法,通过对甘薯栽培技术中不同移栽时间、种植密度、起垄宽度、起垄高度4个因素进行组合试验,进行甘薯增产对比试验研究,以筛选出最佳综合配套技术措施方案,指导大田生产.结果表明:处理5组合的产量最高,与其他各组存在极显著差异.即适时早插,起垄适当,合理施肥是提高甘薯产量的重要栽培管理措施.

  18. 酶法制备紫红薯膳食纤维的工艺研究%Studies on the Preparation Technology of purple Ipomoea Batatas Dietary Fiber by Enzymatic Method

    Institute of Scientific and Technical Information of China (English)

    吴少福; 侯英梅; 上官新晨; 蒋艳; 沈勇根

    2011-01-01

    为了确定酶法制备紫红薯膳食纤维的最优工艺参数,提高产品纯度,以膳食纤维的膨胀力为指标,采用单因素和正交优化试验对酶法制备紫红薯膳食纤维的工艺进行研究.结果表明,将紫红薯渣按1:10(质量比)用水调成浆,糊化后冷却至75℃,按干薯渣的0.5%加入混合酶[m(淀粉酶):m(糖化酶)=7:3],保温处理150min;灭酶后降温至60℃,按原料的0.2%加入木瓜蛋白酶处理60min.样品的酸性洗涤膳食纤维含量达75.46%.该法为紫红薯膳食纤维的制备提供试验依据.%In order to confirm the optimum technologic parameter of purple Ipomoea Batatas dietary fiber prepared by enzymatic method, improving product purity. To make a target of the dietary fiber expansibility, the technology of purple Ipomoea Batatas dietary fiber enzymatic preparation had been researched with single-factor and orthogonal experiment. The results showed that: 1:10 proportion of purple Ipomoea Batatas dregs to water, mixing enzyme (α-amylase:glucoamylase,7:3) amount to dry basis 0.50%, temperature 75 ℃, time 150 min; after thermal inactivation, pawpaw protease amount to dry basis 0.20%, temperature 60 ℃, time 60min. The content of acid detergent DF in the sample reached to 75.46%. Therefore, this technology of enzymatic preparation may lay the foundation of processing for purple Ipomoea Batatas dietary fiber.

  19. Larvicidal efficacy of different plant parts of railway creeper, Ipomoea cairica Extract Against Dengue Vector Mosquitoes, Aedes albopictus (Diptera: Culicidae) and Aedes aegypti (Diptera: Culicidae).

    Science.gov (United States)

    AhbiRami, Rattanam; Zuharah, Wan Fatma; Thiagaletchumi, Maniam; Subramaniam, Sreeramanan; Sundarasekar, Jeevandran

    2014-01-01

    Natural insecticides from plant origin against mosquito vectors have been the main concern for research due to their high level of eco-safety. Control of mosquitoes in their larval stages are an ideal method since Aedes larvae are aquatic, thus it is easier to deal with them in this habitat. The present study was specifically conducted to explore the larvicidal efficacy of different plant parts of Ipomoea cairica (L.) or railway creeper crude extract obtained using two different solvents; methanol and acetone against late third-stage larvae of Aedes albopictus (Skuse) and Aedes aegypti (L.) (Diptera: Culicidae). Plant materials of I. cairica leaf, flower, and stem were segregated, airdried, powdered, and extracted using Soxhlet apparatus. Larvicidal bioassays were performed by using World Health Organization standard larval susceptibility test method for each species which were conducted separately for different concentration ranging from 10 to 450 ppm. Both acetone and methanol extracts showed 100% mortality at highest concentration tested (450 ppm) after 24 h of exposure. Results from factorial ANOVA indicated that there were significant differences in larvicidal effects between mosquito species, solvent used and plant parts (F=5.71, df=2, Paegypti with LC50 of 101.94 ppm followed by Ae. albopictus with LC50 of 105.59 ppm compared with other fractions of I. cairica extract obtained from flower, stem, and when methanol are used as solvent. The larvae of Ae. aegypti appeared to be more susceptible to I. cairica extract with lower LC50 value compared with Ae. albopictus (F=8.83, df=1, Pinsecticide for the control of Aedes mosquitoes. This study quantified the larvicidal property of I. cairica extract, providing information on lethal concentration that may have potential for a more eco-friendly Aedes mosquito control program.

  20. Long-distance dispersal by sea-drifted seeds has maintained the global distribution of Ipomoea pes-caprae subsp. brasiliensis (Convolvulaceae).

    Science.gov (United States)

    Miryeganeh, Matin; Takayama, Koji; Tateishi, Yoichi; Kajita, Tadashi

    2014-01-01

    Ipomoea pes-caprae (Convolvulaceae), a pantropical plant with sea-drifted seeds, is found globally in the littoral areas of tropical and subtropical regions. Unusual long-distance seed dispersal has been believed to be responsible for its extraordinarily wide distribution; however, the actual level of inter-population migration has never been studied. To clarify the level of migration among populations of I. pes-caprae across its range, we investigated nucleotide sequence variations by using seven low-copy nuclear markers and 272 samples collected from 34 populations that cover the range of the species. We applied coalescent-based approaches using Bayesian and maximum likelihood methods to assess migration rates, direction of migration, and genetic diversity among five regional populations. Our results showed a high number of migrants among the regional populations of I. pes-caprae subsp. brasiliensis, which suggests that migration among distant populations was maintained by long-distance seed dispersal across its global range. These results also provide strong evidence for recent trans-oceanic seed dispersal by ocean currents in all three oceanic regions. We also found migration crossing the American continents. Although this is an apparent land barrier for sea-dispersal, migration between populations of the East Pacific and West Atlantic regions was high, perhaps because of trans-isthmus migration via pollen dispersal. Therefore, the migration and gene flow among populations across the vast range of I. pes-caprae is maintained not only by seed dispersal by sea-drifted seeds, but also by pollen flow over the American continents. On the other hand, populations of subsp. pes-caprae that are restricted to only the northern part of the Indian Ocean region were highly differentiated from subsp. brasiliensis. Cryptic barriers that prevented migration by sea dispersal between the ranges of the two subspecies and/or historical differentiation that caused local adaptation

  1. Long-distance dispersal by sea-drifted seeds has maintained the global distribution of Ipomoea pes-caprae subsp. brasiliensis (Convolvulaceae.

    Directory of Open Access Journals (Sweden)

    Matin Miryeganeh

    Full Text Available Ipomoea pes-caprae (Convolvulaceae, a pantropical plant with sea-drifted seeds, is found globally in the littoral areas of tropical and subtropical regions. Unusual long-distance seed dispersal has been believed to be responsible for its extraordinarily wide distribution; however, the actual level of inter-population migration has never been studied. To clarify the level of migration among populations of I. pes-caprae across its range, we investigated nucleotide sequence variations by using seven low-copy nuclear markers and 272 samples collected from 34 populations that cover the range of the species. We applied coalescent-based approaches using Bayesian and maximum likelihood methods to assess migration rates, direction of migration, and genetic diversity among five regional populations. Our results showed a high number of migrants among the regional populations of I. pes-caprae subsp. brasiliensis, which suggests that migration among distant populations was maintained by long-distance seed dispersal across its global range. These results also provide strong evidence for recent trans-oceanic seed dispersal by ocean currents in all three oceanic regions. We also found migration crossing the American continents. Although this is an apparent land barrier for sea-dispersal, migration between populations of the East Pacific and West Atlantic regions was high, perhaps because of trans-isthmus migration via pollen dispersal. Therefore, the migration and gene flow among populations across the vast range of I. pes-caprae is maintained not only by seed dispersal by sea-drifted seeds, but also by pollen flow over the American continents. On the other hand, populations of subsp. pes-caprae that are restricted to only the northern part of the Indian Ocean region were highly differentiated from subsp. brasiliensis. Cryptic barriers that prevented migration by sea dispersal between the ranges of the two subspecies and/or historical differentiation that

  2. Development and Identification of SSR Markers Associated with Starch Properties and β-Carotene Content in the Storage Root of Sweet Potato (Ipomoea batatas L.)

    Science.gov (United States)

    Zhang, Kai; Wu, Zhengdan; Tang, Daobin; Lv, Changwen; Luo, Kai; Zhao, Yong; Liu, Xun; Huang, Yuanxin; Wang, Jichun

    2016-01-01

    Sweet potato (Ipomoea batatas L.) is a nutritious food crop and, based on the high starch content of its storage root, a potential bioethanol feedstock. Enhancing the nutritional value and starch quantity of storage roots are important goals of sweet potato breeding programs aimed at developing improved varieties for direct consumption, processing, and industrial uses. However, developing improved lines of sweet potato is challenging due to the genetic complexity of this plant and the lack of genome information. Short sequence repeat (SSR) markers are powerful molecular tools for tracking important loci in crops and for molecular-based breeding strategies; however, few SSR markers and marker-trait associations have hitherto been identified in sweet potato. In this study, we identified 1824 SSRs by using a de novo assembly of publicly available ESTs and mRNAs in sweet potato, and designed 1476 primer pairs based on SSR-containing sequences. We mapped 214 pairs of primers in a natural population comprised of 239 germplasms, and identified 1278 alleles with an average of 5.972 alleles per locus and a major allele frequency of 0.7702. Population structure analysis revealed two subpopulations in this panel of germplasms, and phenotypic characterization demonstrated that this panel is suitable for association mapping of starch-related traits. We identified 32, 16, and 17 SSR markers associated with starch content, β-carotene content, and starch composition in the storage root, respectively, using association analysis and further evaluation of a subset of sweet potato genotypes with various characteristics. The SSR markers identified here can be used to select varieties with desired traits and to investigate the genetic mechanism underlying starch and carotenoid formation in the starchy roots of sweet potato. PMID:26973669

  3. 能源型甘薯高产栽培技术研究%Research on High Yield Cultivation Techology of Energy Type Ipomoea batatas

    Institute of Scientific and Technical Information of China (English)

    史本林; 尤瑞玲; 李红忠

    2012-01-01

    In order to improve the yield of Ipomoea batatas Lam., and provide technical support for large-scale production, orthogonal test was applied to study the effects of transplanting time, planting density, ridge width and ridging height on the yield of sweet potato. The result showed that the primary-secondary order of the influencing factors was transplanting time, planting density, ridge width, ridging height. The highest yield was obtained in the fifth combination (transplanting on 20th May, planting density of 4.5 ind./m2, ridge width of 70 cm, ridge height of 40 cm). Timely planting early, proper ridge form and rational fertilization are crucial cultivation and management measures.%为了提高甘薯产量,同时为大规模生产提供技术支持,选取甘薯栽培技术中的移栽时间、种植密度、起垄宽度、起垄高度4个因素,每个因素设3个水平进行正交试验研究.结果表明:各因素对产量指标影响的主次依次为移栽时间、种植密度、起垄宽度、起垄高度;各组合中以第5组合(5月20日移栽,种植密度300株/67 m2,起垄宽度70 cm,起垄高度40 cm)的产量最高.从试验结果来看,适时早插,起垄适当,合理施肥是提高甘薯产量的重要栽培管理措施.

  4. Distributions, ex situ conservation priorities, and genetic resource potential of crop wild relatives of sweetpotato [Ipomoea batatas (L. Lam., I. series Batatas

    Directory of Open Access Journals (Sweden)

    Colin Kahlil Khoury

    2015-04-01

    Full Text Available Crop wild relatives of sweetpotato [Ipomoea batatas (L. Lam., I. series Batatas] have the potential to contribute to breeding objectives for this important root crop. Uncertainty in regard to species boundaries and their phylogenetic relationships, the limited availability of germplasm with which to perform crosses, and the difficulty of introgression of genes from wild species has constrained their utilization. Here we compile geographic occurrence data on relevant sweetpotato wild relatives and produce potential distribution models for the species. We then assess the comprehensiveness of ex situ germplasm collections, contextualize these results with research and breeding priorities, and use ecogeographic information to identify species with the potential to contribute desirable agronomic traits. The fourteen species that are considered the closest wild relatives of sweetpotato generally occur from the central United States to Argentina, with richness concentrated in Mesoamerica and in the extreme southeastern United States. Currently designated species differ among themselves and in comparison to the crop in their adaptations to temperature, precipitation, and edaphic characteristics and most species also show considerable intraspecific variation. With 79% of species identified as high priority for further collecting, we find that these crop genetic resources are highly under-represented in ex situ conservation systems and thus their availability to breeders and researchers is inadequate. We prioritize taxa and specific geographic locations for further collecting in order to improve the completeness of germplasm collections. In concert with enhanced conservation of sweetpotato wild relatives, further taxonomic research, characterization and evaluation of germplasm, and improving the techniques to overcome barriers to introgression with wild species are needed in order to mobilize these genetic resources for crop breeding.

  5. Effect of Ipom oea aquatica,as an Ecological Floating Raft,on Water Quality of Aquaculture Ponds%水培空心菜对养殖池塘水质的影响

    Institute of Scientific and Technical Information of China (English)

    王茂元

    2015-01-01

    .47%,43.8%,64.1 %,46.57%,58.75% and 7.4%,respectively.The total yield of the 6-harvest floating plants ranged from 586.86 kg to 1 434.06 kg in the 667 m2 pond.For the 5%,10% and 20% treatments, 1 643.5,988.2 and 4 015.3 g per 667 m2 of TN,respectively,and 240.7,437.6 and 587.9 g per 667 m2 of TP, respectively,were removed from the water. Meanwhile,the survival rate of the tilapia raised in the tested aquaculture pond improved significantly with the treatments.It appeared that cultivating Ipom oea aquatica in a tilapia pond could purify the pond water through eutrophication reduction.Specifically,a 20% of the artificial floating raft on a tilapia aquaculture pond could be recommended for implementation.

  6. MANEJO QUÍMICO DE ESPÉCIES DE CORDA-DE-VIOLA (Ipomoea E Merremia EM ÁREAS DE PRODUÇÃO DE CANA-DE-AÇÚCAR

    Directory of Open Access Journals (Sweden)

    REINERT, Camila Schorr

    2008-01-01

    Full Text Available During the last years it has been observed an increase in the sugarcane areas harvestedmechanically without burning the dry leaves. This system has modified some characteristics of theharvest, since it is left on the soil surface different amount of trash residue, that influence the lightincidence and consequently the occurrence and the way weeds are managed, being the environmentfavorable to the infestation of the species of the genus Ipomoea and Merremia. The increase in theinfestation of the Ipomoeas and Merremias in the sugarcane crop is a motive of concern among Braziliansugarcane growers. The weed reduces the crop yield in the infested areas and interferes with the harvestprocess. The objective of this work is to study the efficacy of the herbicides sulfentrazone, diuron +hexazinone, metribuzin and ametryne + clomazone used in the sugarcane areas without burning in thecontrol of the species Ipomoea and Merremia. For that it was conducted a survey in the areas of greenharvested sugarcane with the application of the herbicides studied and visual observation of the controlwas done at 70 and 90 days after treatment, comparing to the check plot in each commercial block ofsugarcane. The data were analysed using histogram and frequency polygon. The results allowed toconclude that all the herbicides controlled at least 90% of the weed. It was not observed areas with weedcontrol problems after application, mainly for diuron + hexazinone, with a control index of 90%.Nos últimos anos verificou-se um aumento no sistema de colheita mecanizada e semqueima da cana-de-açúcar. Esse sistema modificou algumas características da colheita, uma vez que deixasobre o solo diferentes quantidades de palha, que influem na incidência de luz no local econseqüentemente na ocorrência e nas formas de manejo das plantas daninhas, favorecendo a infestaçãode espécies do gênero Ipomoea e Merremia. O crescimento de focos de cordas-de-viola e Merremias nasplanta

  7. Larvicidal activity of Ipomoea cairica (L.) Sweet and Ageratina adenophora (Spreng.) King & H. Rob. plant extracts against arboviral and filarial vector, Culex quinquefasciatus Say (Diptera: Culicidae).

    Science.gov (United States)

    Samuel, Lallianrawna; Lalrotluanga; Muthukumaran, Rajendra Bose; Gurusubramanian, Guruswami; Senthilkumar, Nachimuthu

    2014-06-01

    Culex quinquefasciatus Say, an arboviral and filarial vector, is one of the most widespread mosquitoes in the world, and insecticide-resistant populations have been reported worldwide. Due to the emergence of resistance in C. quinquefasciatus plant based products or plant extracts may be alternative sources in integrated vector management program. The present study was carried out to establish the larvicidal activities of crude solvent extracts prepared from flowers and leaves of Ipomoea cairica and Ageratina adenophora against third instar larvae of C. quinquefasciatus as target species. The plant extracts were prepared with petroleum ether, chloroform and methanol solvents using sequential extraction method to determine the best extractant for subsequent isolation and characterization of active ingredient. The total yield of plant extract in the Soxhlet extraction ranged between 0.79% and 19.35%. The qualitative phytochemical study of the plant extracts from different solvents showed the presence of alkaloids, flavonoids, phenols, terpenes, saponins and tannins in different combinations. I. cairica and A. adenophora plant extracts were found to be effective against third instar larvae of C. quinquefasciatus causing 77-100% mortality at 48h. Highest mortality was observed at 500ppm and the order of larvicidal action was observed to be of methanol extract of I. cairica flower>petroleum ether extract of A. adenophora leaf>chloroform extract of I. cairica leaf. High mortality (100%) with low LC50 and LT50 were observed in methanolic flower extract (LC50 - 8.43ppm; LT50 - 2.51h at 48h) of I. cairica, and petroleum ether (LC50 - 133.56ppm; LT50 - 9.45h at 48h) leaf extract of A. adenophora. Lethal concentration (LC50 and LC90) values gradually decreased with the exposure periods, lethal time (LT50 and LT90) decreased with the concentration in bioassay experiment with the crude plant extracts. There was a significant correlation (three-way factorial ANOVA) was noticed

  8. Variation Laws of Anthocyanin Content in Roots and Their Relationships with Major Economic Traits in Purple-Fleshed Sweetpotato [Ipomoea batatas (L.) Lam

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Variation laws of anthocyanin content in root during the development and among the varieties, and their relationships with major economic traits in purple-fleshed sweetpotato [Ipomoea batatas (L.) Lam] were studied in the present article. The dynamics of 20 economic traits in 13 purple-fleshed sweetpotato varieties at 20, 40, 60, 80, 100, 120, and 140 d after their transplanting were investigated, and these traits included anthocyanin content in root, length of the longest vine, number of base branches, root number, dry matter contents in stem, foliage and root, fresh/dry weight of root, fresh/dry weight of stem, fresh/dry weight of foliage, fresh/dry weight of stem and foliage, fresh/dry weight of whole plant, and rations of photosynthate to root, stem, and foliage. The correlations between the variations of anthocyanin content and the other 19 economic traits among varieties and during the whole developing stages, and the correlations of daily increase of anthocyanin content with other 10 kinds of yields were analyzed. The results showed that: (1) During the whole development, the anthocyanin content had three variation types, I.e. A slow-increase type, a fluctuating-change type, and a devious- rising type, and had different responses to the growth of length of the longest vine, number of base branches, fresh/dry yield of root, and photosynthate allotments. (2) The anthocyanin contents among 13 varieties began to have significant difference after 20 d, and showed completed differentiation during 40-100 d, which had significantly negative correlationships with the number of base branches, fresh/dry yield of root, photosynthate allotment ratio to root, and had significant positive correlationships with dry matter content of root, length of the longest vine, fresh/dry yield of stem, dry yield of whole plant and photosynthate allotment ratio to foliage. (3) Because of the significantly negative correlation between daily increase of anthocyanin content and dry matter

  9. Hematological effects of Ipomoea batatas(camote) and Phyllanthus niruri(sampa-sampalukan) from Philippines in the ICR mice(Mus musculus)

    Institute of Scientific and Technical Information of China (English)

    Jessa; Fidel; Montejo; Juan; Arturo; Burgos; Mondonedo; Matthew; Genesis; Aguila; Lee; Michael; Bagui; Ples; Rodel; Jonathan; Santos; Vitor; Ⅱ

    2015-01-01

    Objective:To analyze the hematological effects of administering Ipomoea batatas(I.batatas)and Phyllanthus niruri(P.niruri) in the ICR mice.Methods:Powdered leaves of /.batatas and P.nintri were fed to mice for 4 weeks.A total of six groups were used to determine the effect of the plants to the complete blood count of the mouse.Group A(blank control) mice were feed with pellets only;Group B(negative control) mice were fed with pellets coated with honey;Group C(low dosage) mice were fed with honey-coated pellets and powdered leaves of 1.batatas at 10 g/kg body weight of the mouse;Group D(high dosage) mice were fed with honey-coated pellets and powdered leaves of I,batatas at 20 g/kg body weight of the mouse;Group E(low dosage) mice were fed with honey-coated pellets and powdered leaves of P.niruri at 10 g/kg body weight of the mouse:and Group F(high dosage) mice were fed with honey-coated pellets and powdered leaves of P.niruri at 20 g/kg body weight of the mouse.Complete blood count was performed on Days 0.14 and 28.Results:It was shown that I.batatas can increase the values of hematocrit and hemoglobin on both the low dose and high dose at Day 28 and red blood cells(RBC) on both Days 14 and28 of testing.On the other hand.P.niruri can increase RBC.hematocrit and hemoglobin on Day 28 with only the low dose.There were no significant differences with white blood cell,absolute granulocyte,lymphocyte and monocyte,and platelet counts observed for both plant samples.Conclusions:I.batatas and P.niruri have effects on the hematocrit,RBC and hemoglobin levels in mice.

  10. Efeito do déficit hídrico na eficiencia de herbicidas e nas características bioquímicas de Ipomoea grandifolia Effect of water stress on the efficiency of herbicides and on the biochemical characteristics of Ipomoea grandifolia

    Directory of Open Access Journals (Sweden)

    H.S. Vitorino

    2012-03-01

    Full Text Available O objetivo deste estudo foi avaliar a eficácia dos herbicidas inibidores da ALS e PROTOX no controle de Ipomoea grandifolia sob dois níveis de déficit hídrico, bem como determinar a influência desse déficit hídrico sobre o conteúdo de carboidratos solúveis, proteínas totais e aminoácidos livres da planta daninha. O trabalho foi conduzido em casa de vegetação, no delineamento experimental inteiramente casualizado, com os tratamentos dispostos em esquema fatorial 4 x 2, sendo quatro herbicidas (fomesafen, lactofen, chlorimuron-ethyl e imazethapyr e dois níveis de déficit hídrico (com e sem déficit: -0,5 MPa e -0,01 MPa, respectivamente. Aos 7, 14, 21 e 28 dias após a aplicação, o controle foi avaliado de forma visual, e a determinação dos solutos orgânicos foi feita 24, 48, 72 e 96 horas após a aplicação dos herbicidas. À exceção de chlorimuron-ethyl, os demais herbicidas controlaram eficientemente I. grandifolia. A eficiência de controle dos herbicidas foi afetada pela condição de déficit hídrico. Os herbicidas elevaram o conteúdo de carboidratos solúveis, independentemente da condição hídrica, e proporcionaram redução de proteínas solúveis nas plantas de I. grandifolia, bem como aumentaram as concentrações de aminoácidos livres.The objective of this work was to evaluate the efficacy of ALS and PROTOX inhibiting herbicides in the control of Ipomoea grandifolia under two levels of water deficit, as well as to determine the influence of drought on the content of the soluble carbohydrates, protein and free amino acids of the weed. The study was conducted in a greenhouse in a completely randomized design with treatments in a factorial scheme 4 x 2, with four herbicides (fomesafen, lactofen, chlorimuron-ethyl and imazethapyr, and two levels of water deficit (with and without deficit, - 0.5 MPa and -0.01 MPa, respectively. At 7, 14, 21, and 28 days after application, the control was evaluated visually and

  11. Interferência e nível de dano econômico de Brachiaria plantaginea e Ipomoea nil na cultura do feijão comum Interference and economic threshold of Brachiaria plantaginea and Ipomoea nil in the common bean crop

    Directory of Open Access Journals (Sweden)

    Ribas Antonio Vidal

    2010-08-01

    Full Text Available As pesquisas acerca do impacto da interferência das plantas daninhas sobre as culturas normalmente são realizadas com uma única espécie infestante, quando na realidade podem ocorrer diversas. Os objetivos deste trabalho foram avaliar a interferência de Brachiaria plantaginea e Ipomoea nil sobre o rendimento de grãos do feijoeiro (Phaseolus vulgaris e determinar o nível de dano econômico (NDE de cada espécie na cultura. Foram realizados dois experimentos a campo com as cultivares de feijão 'IPR Graúna' (Grupo Preto e 'UTF-06' (Grupo Carioca em convivência com infestações mistas em diferentes proporções aleatórias das espécies B. plantaginea e I. nil. Modelos de regressão múltiplos foram utilizados para determinar a capacidade de interferência de cada espécie daninha sobre a produtividade da cultura. O impacto de interferência de cada planta de I. nil correspondeu a 2,6 e 0,25 quando comparada a cada planta de B. plantaginea, dependente das proporções relativas na comunidade infestante e das condições de cada experimento. A cultivar 'IPR Graúna' apresentou maior habilidade competitiva do que 'UTF-06'. O NDE foi dependente da proporção relativa de cada espécie daninha na comunidade e foi obtido sob baixas infestações (The researches about the impact of weed interference on crops are usually performed with a single weed species, when in reality there are numerous. The objective of this study was to evaluate the interference of Brachiaria plantaginea and Ipomoea nil on the grain yield of French beans (Phaseolus vulgaris and to determine the economic weed threshold (EWT of each species on the crop. Two field experiments were conducted with bean cultivars 'IPR Graúna' (Black group and 'UTF-06' (Carioca group in coexistence with random proportions of mixed infestations of B. plantaginea and I. nil. Multiple regression models were used to determine the interference abi1lity of each weed species on crop productivity. The

  12. 广东地区外来种五爪金龙的传粉生物学研究%Pollination biology of an invasive weed Ipomoea cairica (Convolvulaceae) in Guangdong Province, China

    Institute of Scientific and Technical Information of China (English)

    贾效成; 李新亮; 丹阳; 路国辉; 王英强

    2007-01-01

    五爪金龙(Ipomoea cairica)是一种匍匐或攀援生长的入侵杂草.2006-2007年作者在广东地区开展了对该入侵种的传粉生物学研究.五爪金龙在广东全年均可开花,花序为聚伞花序,花冠为紫色或蓝紫色喇叭状.开花时间在早晨4: 30-5: 20之间,下午17: 40闭合,持续大约12 h.每朵花平均分泌花蜜量为5.82 ± 2.47 μL (n = 10),花蜜平均含糖量为41.03 ± 6.52%,氨基酸含量为0.042 mg/mL.开花过程中花粉活性和柱头可受性均保持较高水平(》90%).花粉/胚珠比率(P/O)为4,778.18 ± 264.58 (n = 10).木蜂(Xylocopa sp.)是其有效的传粉昆虫,蝇类和蝶类为随机访问者.开花过程中柱头与花药相接触,有利于自花授粉.然而,繁育系统的研究表明五爪金龙是自交不亲和植物,其种子仅能通过异花授粉获得.%During May to July, 2006 and April to May, 2007, we studied pollination biology in Ipomoea cairica, an invasive weed in Guangdong Province, China. Ipomoea cairica is a perennial creeping or climbing herbaceous vine, blooming all year round in Guangdong. The flowers gathered in cymes, with a purple or bluish purple bell-formed corolla. The petals unfolded at about 4:30-5:20 and closed at 17:40, lasting for about 12 hours. The proximity of the stigma and anthers during flowering period facilitated the transfer of self-pollen. The mean volume of nectar per flower was 5.82 ± 2.47 μL (n = 10), with a sugar concentration of 41.03 ± 6.52% and an amino acid concentration of ca. 0.042 mg/mL. During anthesis, both pollen viability and stigma receptivity remained at a high level (>90%). The mean P/O ratio was 4,778.18 ± 264.58 (n = 10). Carpenter Bees (Xylocopa sp.) were considered as effective pollinators, while flies and butterflies were occasional visitors. Ipomoea cairica is self-incompatible as determined by an absence of fruit set in the flowers following controlled hand self-pollination, producing fruits and viable seeds only after

  13. 厚藤Ipomoep Pes-Caprae(L.) Sweet的化学成分研究(Ⅰ)%Study on the chemical constituents of Ipomoea Pes-caprae (L.) Sweet (Ⅰ)

    Institute of Scientific and Technical Information of China (English)

    王清吉; 王友绍; 何磊; 张偲

    2006-01-01

    目的从厚藤(Ipomoea Pes-Caprae(L.)Sweet)的乙酸乙酯提取物中分离到7个化合物.方法采用柱层析技术、经理化常数和波谱分析进行结构鉴定.结果它们分别是β-香树素(1)、12-ursen-3-ol(2)、β-谷甾醇(3)、豆甾醇(4)、α-菠甾醇(5)、异莨菪亭(6)和七叶内酯(7).结论其中异莨菪亭和七叶内酯为首次从该植物中分离得到.

  14. Purification and Characterization of a Lectin from Variety of Ipomoea Batatas%甘薯品种岩8-6凝集素的分离纯化及其性质

    Institute of Scientific and Technical Information of China (English)

    林晓红; 余萍; 林少琴; 林曦

    2000-01-01

    甘薯品种岩8-6的叶片组织依次经硫酸铵分级沉淀、甲壳素亲和层析及Sephadex G-75分离纯化得到一种凝集素(Ipomoea Batatas Lectin,简称IBL).纯化的IBL电泳显示一条蛋白质着色带.IBL的亚基相对分子质量为47000.IBL对兔红细胞有凝集作用,凝血活性可被D-果糖明显抑制.IBL 对热不稳定,加热到70℃时,活性完全丧失.IBL对碱敏感.IBL含有16种氨基酸,富含Asp和Glu,中性糖含量为5.07%.

  15. 用2,4-D处理获得可育甘薯组种间杂种%Production of Fertile Interspecific Hybrids of Ipomoea Section batatas by 2,4-D Treatment

    Institute of Scientific and Technical Information of China (English)

    翟红; 刘庆昌

    2002-01-01

    用50mg/L 2,4-D处理杂交不亲和组合甘薯(Ipomoea batatas (L.)Lam.)品种徐薯18(2n=6x=90)×L.lacunosa(2n=2x=30)以及I.trifida(2n=4x=60)×L triloba(2n=2x=30)的母本花器,获得了可育种间杂种植株,并对种间杂种进行了过氧化物酶同工酶分析和细胞学观察.特别是徐薯18×I.lacunosa的杂种后代具有明显的结薯性,这是首次报道获得具有结薯性的甘薯品种和第Ⅱ群近缘野生种种间杂种.

  16. Photosynthesis and fluctuating asymmetry as indicators of plant response to soil disturbance in the Fall-Line Sandhills of Georgia: a case study using Rhus copallinum and Ipomoea pandurata

    Science.gov (United States)

    Freeman, D. Carl; Brown, Michelle L.; Duda, Jeffrey J.; Graham, John H.; Emlen, John M.; Krzysik, Anthony J.; Balbach, Harold E.; Kovacic, David A.; Zak, John C.

    2004-01-01

    We examined net photosynthesis, transpiration, stomatal conductance, and leaf fluctuating asymmetry on two species (Rhus copallinum and Ipomoea pandurata) as indicators of stress at nine sites across a gradient of soil disturbance at Fort Benning, Georgia. There were three sites for each of three disturbance levels. Physical habitat disturbance was caused by activities associated with infantry training, including mechanized elements (tanks and personnel carriers) and foot soldiers. In addition, we examined the influence of prescribed burns and microhabitat effects (within meter‐square quadrats centered about the plant) on these measures of plant stress. Net photosynthesis declined with increasing disturbance in the absence of burning for both species. However, when sites were burned the previous year, net photosynthesis increased with increasing disturbance. Developmental instability in Rhus, as measured by fluctuating asymmetry, also declined with increasing disturbance in the absence of burning but increased with disturbance if sites were burned the previous year. Developmental instability was much less sensitive to burning in Ipomoea and in general was lowest at intermediate disturbance sites. Microenvironmental and microhabitat effects were weakly correlated with measures of plant stress when all sites were combined. However, higher correlations were obtained within site categories, especially when the recent history of prescribed burning was used as a category. Finally, using all of the combined data in a discriminant function analysis allowed us to correctly predict the disturbance level of more than 80% of the plants. Plant stress is responsive to both large‐scale perturbations, such as burning, and microhabitat parameters. Because of this, it is important to include macro‐ and microhabitat parameters when assessing stress. Similarly, we found a combination of developmental and physiological indicators of stress was superior to using them

  17. Optimization of Microwave-Assisted Extraction Technology of Flavonoids from Ipomoea batatas Leaves by Response Surface Methodology%甘薯叶黄酮微波提取工艺的响应面法优化

    Institute of Scientific and Technical Information of China (English)

    韦学丰; 罗静

    2013-01-01

    Response surface analysis was applied to optimize the microwave-assisted extraction conditions of flavonoids from Ipomoea batatas Lam leaves.The effects of microwave power,microwave time,ethanol volume fraction,liquid to solid ratio on the extraction yield of flavonoids were studied.And the extraction process was optimized by Box-Behnken design.The results showed that the optimum extraction conditions were microwave power,200 W; microwave time,4 min; volume fraction of ethanol,50%; and liquid to solid ratio 43:1 (V/m,mL:g).Under these conditions,the extraction yield of flavonoids of I.batatas leaves was 8.082 7%,which was accord with the predicted value(8.314 2%).%用响应面法优化甘薯(Ipomoea batatas)叶黄酮的微波提取工艺,研究了微波功率、微波时间、乙醇体积分数、液固比4个因素对黄酮提取率的影响.并采用Box-Behnken设计对最佳提取工艺进行了优化.结果表明,最佳工艺条件为微波功率200 W、微波时间4 min、乙醇体积分数50%、液固比43∶1(V/m,mL∶g),在此条件下甘薯叶黄酮的提取率为8.082 7%,与预测值8.314 2%基本吻合.

  18. Study on the Quick-Freezed Mud Production with the Flavor of Baked Ipomoea batatas Lam%速冻烤紫薯风味泥制品的研制

    Institute of Scientific and Technical Information of China (English)

    梁曹雯; 邓后勤

    2016-01-01

    以紫薯为原料,通过设置温度梯度研究了烤制温度对紫薯花色苷的影响,得出最佳烘烤温度,通过正交试验研究了配料比对烤紫薯风味调理制品质量的影响,确定产品的最优配方。结果表明:最佳烘烤温度为200℃;产品最优配方为:100 g紫薯泥,添加糖粉2.0%、吉士粉2.0%、奶粉2.5%、黄油4.0%,该配方生产的速冻烤紫薯风味泥制品紫薯风味浓郁,口感细腻。%UsingIpomoea batatas Lam as material, researched the effect of baking temperature on the anthocyanins by setting the temperature gradient, and obtained the optimum baking temperature; studied the effect of ratio of ingredients on the quantity of conditioning products with the lfavor of baked purple sweet potato by the method of orthogonal experiment, and determined the optimum formula of products. The results showed that the best baking temperature was 200℃; the optimal formula for the products was each 100g purple potato mud, adding 2.0% of powdered sugar, 2.0% of custard powder, 2.5% of milk powder and 4.0% of butter. With this formula, the quick-freezed mud production with the lfavor of bakedIpomoea batatas Lam presented rich lfavor and delicate taste.

  19. Ocorrência de Brevipalpus phoenicis (Geijskes (Acari, Tenuipalpidae, Tetranychus urticae (Koch (Acari, Tetranychidae e Polyphagotarsonemus latus (Banks (Acari, Tarsonemidae sobre folhas de Ipomoea cairica (Linnaeus Sweet (Solanales, Convolvulaceae Occurrence of Brevipalpus phoenicis (Geijskes (Acari, Tenuipalpidae, Tetranychus urticae (Koch (Acari, Tetranychidae and Polyphagotarsonemus latus (Banks (Acari, Tarsonemidae on leaves of I. cairica (Linnaeus Sweet (Solanales, Convolvulaceae

    Directory of Open Access Journals (Sweden)

    Ozana M. de A. Maia

    2006-09-01

    Full Text Available A ocorrência de três espécies acarinas fitófagas é relatada pela primeira vez sobre folhas de Ipomoea cairica. As espécies Brevipalpus phoenicis (Geijskes, Tetranychus urticae (Koch e Polyphagotarsonemus latus (Banks, foram coletadas sobre folhas de I. cairica nas imediações da Universidade Federal do Paraná, Curitiba, Paraná, Brasil, em 20 de janeiro de 2005.The first occurrence of three phytophagus mites on Ipomoea cairica, is reported. The species Brevipalpus phoenicis (Geijskes, Tetranychus urticae (Koch and Polyphagotarsonemus latus (Banks were caught on leaves of I. cairica, around Universidade Federal do Paraná, Curitiba, Paraná, Brazil, in January 20th, 2005.

  20. Aversão alimentar condicionada para o controle da intoxicação por Ipomoea carnea subsp. fistulosa em caprinos

    Directory of Open Access Journals (Sweden)

    Luciano A. Pimentel

    2013-06-01

    Full Text Available A aversão alimentar condicionada é uma técnica que pode ser utilizada em animais para evitar a ingestão de plantas tóxicas. O presente estudo teve como objetivo testar a eficiência e durabilidade da aversão alimentar condicionada em caprinos para evitar o consumo de Ipomoea carnea subsp. fistulosa. Foram utilizados 14 caprinos jovens da raça Moxotó, que foram adaptados ao consumo da planta. Inicialmente foi administrada I. carnea subsp. fistulosa dessecada e triturada misturada à ração concentrada por 30 dias e, posteriormente, foi fornecida a planta verde por mais 10 dias. Para constatação da adaptação ao consumo da planta os caprinos foram colocados a pastar em um piquete de 510 m² onde tinha sido plantada I. carnea subsp. fistulosa em uma área de 30m² (10 plantas/m². No 42º dia de experimento, após a constatação do consumo espontâneo os animais receberam a planta verde individualmente na baia por alguns minutos, e todos os animais que consumiam qualquer quantidade da planta foram tratados com uma solução de LiCl na dose 175mg por kg de peso vivo. Este procedimento repetiu-se por mais dois dias. Posteriormente, os caprinos foram divididos em dois grupos: Grupo 1 com seis animais, quatro deles avertidos e dois não avertidos (facilitadores; e o Grupo 2, com oito caprinos, todos avertidos. Para constatar a eficiência e duração da aversão e a influência de animais facilitadores na durabilidade da aversão, os caprinos foram colocados a pastar, em dias alternados, três dias por semana, durante duas horas, no piquete plantado com I. carnea subsp. fistulosa. Por 12 meses os animais foram monitorados durante o pastejo, identificando-se o consumo e a preferência dos animais pelas plantas presentes no piquete. No Grupo 1 tanto os caprinos avertidos quanto os não avertidos iniciaram a ingerir a planta em 1-6 semanas e gradualmente foram aumentando a planta consumida, mas nunca a ingeriram exclusivamente. Nenhum caprino

  1. Doença de depósito lisossomal induzida pelo consumo de Ipomoea verbascoidea (Convolvulaceae em caprinos no semiárido de Pernambuco

    Directory of Open Access Journals (Sweden)

    Dayane D.C.C. Lima

    2013-07-01

    Full Text Available O objetivo deste trabalho foi reproduzir a intoxicação por Ipomoea verbascoidea em caprinos e descrever os aspectos epidemiológicos, clínicos e histopatológicos da intoxicação espontânea por essa planta no Estado de Pernambuco. Para isso, realizou-se o acompanhamento da epidemiologia da doença em sete municípios do semiárido pernambucano. Três caprinos espontaneamente intoxicados foram examinados e, em seguida eutanasiados e necropsiados (Grupo I. Para reproduzir experimentalmente a doença, as folhas secas de I. verbascoidea contendo 0,02% de swainsonina, foram fornecidas na dose de 4g/kg (0,8mg de swainsonina/kg a dois grupos de três animais. Os caprinos do Grupo II receberam a planta diariamente por 40 dias e foram eutanasiados no 41º dia de experimento. Os caprinos do Grupo III receberam a planta diariamente por 55 dias e foram eutanasiados no 120º dia de experimento. Outros três caprinos constituíram o grupo controle (Grupo IV. Nos grupos experimentais, as lesões encefálicas foram avaliadas por histopatologia e adicionalmente avaliaram-se as lesões cerebelares por morfometria, mediante mensuração da espessura da camada molecular, do número de neurônios de Purkinje e da área dos corpos celulares dessas células. Os principais sinais clínicos e lesões microscópicas foram semelhantes aos previamente reportados em animais intoxicados por plantas que contem swainsonina. Nos caprinos do GII e GIII, os primeiros sinais clínicos foram observados entre o 22º e 29º dia de experimento; clinicamente a doença desenvolvida por esses animais foi semelhante aos casos espontâneos. Nenhum dos caprinos do GIII se recuperou dos sinais neurológicos. Esse resultado evidencia que o consumo da planta por 26-28 dias após a observação dos primeiros sinais clínicos é suficiente para provocar lesões irreversíveis. Pela análise morfométrica, a camada molecular do cerebelo dos caprinos do Grupo I e III eram mais delgadas que

  2. Aversão alimentar condicionada no controle de surtos de intoxicações por Ipomoea carnea subsp. fistulosa e Turbina cordata em caprinos

    Directory of Open Access Journals (Sweden)

    Luciano A. Pimentel

    2012-08-01

    Full Text Available A aversão alimentar condicionada é uma técnica que pode ser utilizada em animais para evitar a ingestão de plantas tóxicas. A técnica foi utilizada em uma fazenda para controlar a intoxicação por Turbina cordata e em outra para Ipomoea carnea subsp. fistulosa. Os caprinos eram presos à noite, e na manhã do dia seguinte lhes era ofertada a planta verde, recém-colhida, por dez minutos. Os caprinos que ingerissem qualquer quantidade da planta eram identificados, pesados e tratados com LiCl na dose de 175mg/kg peso vivo através de sonda esofágica. No rebanho da fazenda na que havia T. cordata a técnica foi aplicada a cada dois meses durante o período em que a planta é encontrada. Durante todo o experimento, de dezembro de 2009 a abril de 2011 não ocorreu nenhum novo caso de intoxicação no rebanho e diminuiu gradualmente o número de animais avertidos e a quantidade de planta que ingeriam os mesmos durante o processo de aversão. Na fazenda na que ocorria intoxicação por I. carnea a maioria de rebanho foi avertido em dezembro de 2010, 15-20 dias antes do início das chuvas, e os animais não ingeriram a planta espontaneamente no campo até setembro-outubro de 2011, durante o período da seca, quando havia extrema carência de forragem e iniciaram a ingerir a planta no campo. Posteriormente, apesar de três tratamentos aversivos com 21 dias de intervalo, os animais continuaram a ingerir a planta e ocorreram casos clínicos. A técnica de aversão alimentar condicionada demonstrou ser eficiente e viável para o controle da intoxicação por T. cordata. Para a intoxicação por I. carnea a técnica impediu a ingestão da planta somente durante a época de chuvas, mas não durante a seca, quando há pouca disponibilidade de forragem. A diferença nos resultados com as duas plantas é, aparentemente, resultante das condições epidemiológicas diferentes nas que ocorrem as intoxicações. T. cordata desaparece durante a maior parte do

  3. The Influence of the Ratio of Nitrate to Ammonium Nitrogen on Nitrogen Removal in the Economical Growth of Vegetation in Hybrid Constructed Wetlands

    Directory of Open Access Journals (Sweden)

    Haq Nawaz Abbasi

    2017-03-01

    Full Text Available Growing vegetables economically in the use of constructed wetland for wastewater treatment can play a role in overcoming water and food scarcity. Allium porrum L., Solanum melongena L., Ipomoea aquatica Forsk., and Capsicum annuum L. plants were selected to grow in hybrid constructed wetland (CW under natural conditions. The impact of the ratio of nitrate to ammonium nitrogen on ammonium and nitrate nitrogen removal and on total nitrogen were studied in wastewater. Constructed wetland planted with Ipomoea aquatica Forsk. and Solanum melongena L. showed higher removal efficiency for ammonium nitrogen under higher ammonium concentration, whereas Allium porrum L.-planted CW showed higher nitrate nitrogen removal when NO3–N concentration was high in wastewater. Capsicum annuum L.-planted CW showed little efficiency for both nitrogen sources compared to other vegetables.

  4. Effects of feeding sweet potato (Ipomoea batatas) vines as a supplement on feed intake, growth performance, digestibility and carcass characteristics of Sidama goats fed a basal diet of natural grass hay.

    Science.gov (United States)

    Megersa, Tadesse; Urge, Mengistu; Nurfeta, Ajebu

    2013-02-01

    The objective of this study was to investigate the effects of substituting sweet potato [Ipomoea batatas (L) Lam] vines for concentrate on growth performance, digestibility, and carcass characteristics. Thirty yearling bucks (15.3 ± 1.64 kg) were assigned into six treatments in a randomized complete block design: natural grass hay alone (T1) or supplemented with 100 % sweet potato vines (SPV) (T2), 65 % SPV + 35 % concentrate (T3), 35 % SPV + 65 % concentrate (T4), and 100 % concentrate (T5) on dry matter (DM) basis. Supplemented goats (T2, T3, T4, and T5) consumed higher (p goats (349 g/day). The crude protein (CP) intake (32.0, 48.6, 54.7, and 69.2 g/day) increased with increasing levels of the concentrate in the diet for T2, T3, T4, and T5, respectively. The DM digestibility in T2, T3, T4, and T5, respectively, was higher (P goats lost weight (-19.5 g/day). Slaughter weight, empty body weight, hot carcass weight, dressing percentage, rib-eye muscle area, and total edible offals were higher (P goats compared with nonsupplemented ones. Therefore, it could be concluded that sweet potato vine can replace the conventional concentrate and could be fed with poor quality hay to prevent body weight loss of animal in the absence of other feed supplements.

  5. 厚藤Actin基因片段的克隆及序列分析%Cloning and Sequence Analysis of Actin Gene Fragment from Ipomoea pes-caprae L

    Institute of Scientific and Technical Information of China (English)

    郭艳; 张美; 夏快飞; 简曙光; 陈建通

    2016-01-01

    为研究功能基因在厚藤(Ipomoea pes-caprae L)中的表达和调控提供内参基因,本文根据Actin基因的保守区设计简并性引物,采用RT-PCR克隆厚藤的Actin基因片段,然后将获得的片段连接于克隆载体上进行测序,并运用分子生物学软件对该基因序列进行分析.结果表明,该基因片段大小为554 bp,编码184个氨基酸;该序列与其他植物Actin基因的cDNA序列的同源性均在80%以上,与氨基酸序列的同源性在94%以上.由此得出,本研究克隆的基因序列为Actin基因片段,将其命名为IpActin1,并登录在GenBank,登录号为KU564627.

  6. EXTRACTION AND CHARACTERIZATION OF THE LECTIN OF THE IPOMOEA BATATAS F3282%甘薯品种F3282凝集素的提取及性质研究

    Institute of Scientific and Technical Information of China (English)

    余萍; 林少琴; 林玉满; 林晓红

    2000-01-01

    甘薯品种F3282的浸取液,经硫酸铵分级,甲壳素层析后,再经分子筛过滤,获得在PAGE上呈现单一蛋白质带的甘薯凝集素(Ipomoea Batatas Lectin,IBL).IBL对鸽、兔、人血等红细胞均有凝集作用,其中对鸽血的凝集活性最高,最低凝集浓度为0.05 μg/ml.IBL除凝集红细胞外,还可凝集小鼠脾细胞和肿瘤细胞;其对热相当稳定,90℃加热10min,仍有部分活性;IBL对酸处理较碱处理稳定,但可被阿拉伯糖或乳糖等解除凝集活性.IBL的中性糖含量为5.4%.

  7. 紫色甘薯营养成分和药用价值研究进展%Research Progress on Nutritive Component and Medicinal Value of Purple Sweet Potato [Ipomoea batatas (L.) Lam.

    Institute of Scientific and Technical Information of China (English)

    温桃勇; 刘小强

    2009-01-01

    紫色甘薯[Ipomoea batatas(L.)Lam]是一种富含天然食用色素的独特甘薯.从20世纪90年代初在日本农林水产省九州农业试验场选育出的"川山紫"开始,紫色甘薯由于富含多种营养成分,具有清除自由基抗氧化、预防和治疗心血管疾病等多种药用功能而在日本等发达国家得到广泛推广.就国内外对紫色甘薯各方面的研究,对其营养成分和药用价值进行论述,为在国内广泛推广种植紫色甘薯新品种提供重要依据.

  8. Aquaculture Asia, Vol.13, No.2, pp.1-56, April-June 2008

    OpenAIRE

    2008-01-01

    Peter Edwards writes on rural aquaculture: From integrated carp polyculture to intensive monoculture in the Pearl River Delta, South China. Better management practices for Vietnamese catfish. Ipomoea aquatica – an aquaculture friendly macrophyte. A status overview of fisheries and aquaculture development in Pakistan with context to other Asian countries. The changing face of post-grad education in aquaculture: contributing to soaring production and sustainable practices. H...

  9. Leaf vegetables for use in integrated hydroponics and aquaculture systems: Effects of root flooding on growth, mineral composition and nutrient uptake

    DEFF Research Database (Denmark)

    Trang, Ngo Thuy Diem; Schierup, Hans-Henrik; Brix, Hans

    2010-01-01

    In recirculating aquaculture and hydroponics systems, the waste products from fish production are used to produce vegetables or other crops of economic value, and the water is recirculated to the fish tanks. We studied growth, productivity and nutrient uptake of four leaf vegetable species (Lactuca...... sativa, Ipomoea aquatica, Brassica rapa var. chinensis and Brassica rapa var. parachinensis) in a controlled growth experiment with three root flooding treatments (drained, half-flooded and flooded) to assess their preferred hydroponic growth requirements, biomass production and nutrient removal...... capacities. Growth of the two Brassica varieties was clearly best at drained root conditions, while L. sativa and I. aquatica grew best with half-flooded and flooded roots. I. aquatica took up 3 times more N, P and K per plant than L. sativa, and 4 to 6 times more than the two Brassica varieties. At a plant...

  10. Produção da batata-doce adubada com esterco bovino e biofertilizante Yield of sweet potato (Ipomoea batatas (L. Lam. fertilized with cattle manure and biofertilizer

    Directory of Open Access Journals (Sweden)

    Ademar Pereira de Oliveira

    2007-12-01

    Full Text Available Objetivou-se avaliar a produção da batata-doce (Ipomoea batatas (L. Lam. adubada com esterco bovino e biofertilizante, conduziu-se um experimento no período de Janeiro a Junho de 2004, em NEOSSOLO REGOLÍTICO, psamítico típico, na Universidade Federal da Paraíba, em Areia-PB. O delineamento experimental empregado foi de blocos casualisados, com quatro repetições com os tratamentos distribuídos em arranjo fatorial 6 x 2, sendo seis doses de esterco bovino (0, 10, 20, 30, 40, 50 t ha-1 e ausência e presença de biofertilizante. As parcelas foram constituídas por 80 plantas, espaçadas de 80 x 30 cm. O número de raízes comerciais por planta atingiu valores máximos estimados de 2,0 e 1,7 raízes, respectivamente, nas doses de 26 e 25 t ha-1 de esterco bovino na presença e ausência de biofertilizante. O peso médio de raízes comerciais da batata-doce aumentou com elevação das doses de esterco bovino, atingindo na maior dose 360 e 224 g na presença e ausência de biofertilizante. As doses de 25,6 e 24,4 t ha-1 de esterco bovino foram responsáveis pela máxima produção total de raízes de 21,4 e 21,2 t ha-1, respectivamente, na presença e ausência de biofertilizante. As máximas produções de raízes comerciais, 15,2 e 12,9 t ha-1 foram alcançadas com 25,5 e 21,3 t ha-1 de esterco bovino, na presença e ausência de biofertilizante, respectivamente.Aiming to evaluate the sweet potato (Ipomoea batatas (L. Lam. yield fertilization with bovine manure and biofertilizer, an experiment was carried out from January to July, 2004, on ustipsamment soil, at Universidade Federal da Paraíba, Areia-PB. The Experimental outline used was randomized blocks, with four replications and, treatments distributed in factorial arrangement 6 x 2, being six levels of bovine manure (0, 10, 20, 30, 40, 50 t ha-1 and absence and presence of biofertilizer. Plots were constituted by 80 plants, spaced 80 x 30 cm. Commercial roots number per plant reached

  11. Potencial productivo de esquejes de boniato (Ipomoea batatas (L. Lam. procedentes de una nueva tecnología de producción de semilla agámica.

    Directory of Open Access Journals (Sweden)

    Roberto Díaz Hernández

    2012-01-01

    Full Text Available RESUMEN El trabajo se realizó en el Instituto de Investigaciones en Viandas Tropicales durante el periodo de septiembre del 2008 a abril 2009, con los objetivos de determinar la cantidad de esquejes que proporciona el vivero y la frecuencia de corte, así como comparar el potencial productivo de cada corte. El Experimento consistió en el montaje de cámaras de aproximadamente 1m de ancho y 15m de largo, se utilizaron raíces tuberosas del clon comercial INIVIT B2-2005 como material de plantación. Se comprobó que durante la fase del vivero este proporcionó un total de nueve cortes tanto en la época frío como en primavera con un total de 764 y 1390 esquejes respectivamente. Después de cortados los esquejes fueron llevados al campo donde se realizó un estudio para determinar los rendimientos correspondientes a cada tratamiento (número de cortes. Se utilizó un diseño de bloques al azar con cuatros réplicas. Los resultados mostraron que en pequeñas áreas de viveros se puede obtener un número significativo de esquejes y de muy buena calidad productiva además de que los esquejes procedentes de diferentes momentos de cortes presentan igual comportamiento en cuanto al rendimiento. Productive potential of sweetpotato (Ipomoea batatas (L. Lam cuttings from a new technology for agamic seed production. ABSTRACT The work was conducted at the Research Institute of Tropical Root and Tuber Crops from September 2008 to April 2009 with the objectives of determining the amount of cuts provided by the nursery and cut frequency, as well as, to compare the productive potential of each cut. The experiment consisted of mounting chambers about 1m wide and 15m long. Tuberous roots from the commercial clone INIVIT B2-2005 were used as planting seeds. During the nursery stage, nine cuts were provided in the winter and rainy seasons with a total of 764 and 1390 cuttings respectively. Cuttings were transferred to field conditions to determine corresponding

  12. Carfentrazone-ethyl aplicado em pós-emergência para o controle de Ipomea spp. e Commelina benghalensis na cultura da cana-de-açúcar Carfentrazone-ethyl applied in post-emergence to control Ipomoea spp. and Commelina benghalensis in sugarcane crop

    Directory of Open Access Journals (Sweden)

    P.J. Christoffoleti

    2006-01-01

    Full Text Available Este trabalho teve como objetivos determinar a eficácia e seletividade do carfentrazone-ethyl no controle de plantas daninhas na cultura da cana-de-açúcar e avaliar curvas de dose-resposta desse herbicida em Ipomoea spp. e Commelina benghalensis. Desenvolveu-se um ensaio de campo, em pós-emergência das plantas daninhas e da cultura (cv. RB72-454. O delineamento experimental utilizado foi do tipo blocos ao acaso, com os seguintes tratamentos: carfentrazone a 5, 10, 20, 30 e 50 g ha-1 e metribuzin a 2.400 g ha-1. As plantas daninhas que infestavam a área eram Ipomoea nil, I. grandifolia, I. quamoclit, Momordica charantia e C. benghalensis. As avaliações de controle visual e fitotoxicidade foram realizadas aos 15, 30 e 45 dias após a aplicação dos herbicidas. Para elaboração das curvas de dose-resposta, um experimento foi instalado em casa de vegetação, com as plantas daninhas I. nil, I. hederifolia, I. grandifolia, I. quamoclit e C. benghalensis. As doses do herbicida carfentrazone foram: 10D, 4D, 2D, D, 0,5D, 0,25D, 0,125D, 0,1D e 0,01D, em que D = 20 g ha¹. Os dados foram analisados a partir de curvas de dose-resposta de controle visual e massa fresca. No experimento de campo, observou-se excelente controle das plantas daninhas, sendo a dose de 50 g ha-1 de carfentrazone a mais eficiente. Nenhum tratamento causou danos à cana-de-açúcar. O experimento de casa de vegetação permitiu concluir que a ordem decrescente de sensibilidade das espécies de corda-de-viola ao carfentrazone-ethyl é: I. hederifolia > I. quamoclit > I. nil > I. grandifolia; e que C. benghalensis é controlada pelo carfentrazone a partir da dose de 5 g ha-1.This work aimed to evaluate the efficacy and selectivity of the herbicide carfentrazone-ethyl applied in post-emergence to control weeds in sugarcane crop and to evaluate dose-response curves of this herbicide in four species of Ipomoea spp. and Commelina benghalensis. Field experiment was carried out in

  13. Study on Chemical Constituents and Antioxidant Activity of Anthocyanins from Ipomoea batatas L.(purple sweet potato)%紫甘薯花色苷的组分及抗氧化活性研究

    Institute of Scientific and Technical Information of China (English)

    朱洪梅; 赵猛

    2009-01-01

    Anthocyanins extracted from purple sweet potatoes (Ipomoea batatas L.) were purified through AB-8 resin column. The extract contains 11 kinds of anthocyanins by HPLC-DAD analysis. The majority of the anthocyanins are acylated cyanidin and peonidin. The objective of this study was to investigate the antioxidant activities of anthocyanins from purple sweet potatoes by different analytical methods: the α,α-diphenyl-β-picrylhydrazyl (DPPH) radical-scavenging effect and superoxide anions radical-scavenging effect, reducing power and antioxidant effect on lipid peroxidation in a linoleic acid model system. At a concentration of 0.5g/L, the reducing power of anthocyanins, L-ascorbic acid (L-AA) and butylated hydroxytoluene (BHT) reached 0.572, 0.460 and 0.121, respectively. The result displayed that anthocyanins possessed excellent reducing power. The fact that half inhibition concentrations (IC50) of anthocyanins scavenging DPPH radical and superoxide anions radical were 6.94 and 3.68mg/Lrespectively indicates that anthocyanins were effective in scavenging DPPH and superoxide anions radical. In addition, anthocyanins could significantly inhibit the peroxidation of lipid.%对紫甘薯花色苷的化学成分和抗氧化活性进行了研究.研究采用大孔树脂AB-8纯化紫甘薯花色苷,高效液相色谱-2极管阵列法(HPLC-DAD)分析表明,纯化后的提取物中共含有11种花色苷,其中主要成分为酰化的矢车菊素和芍药素.并测定了紫甘薯总花色苷在DPPH自由基清除体系、超氧阴离子体系、还原力和亚油酸体系的抗氧化活性.在质量浓度均为0.5g/L时,花色苷、L-AA和 BHT的还原力分别为0.572、0.460 和0.121,花色苷的清除DPPH自由基的半数抑制浓度(IC50)和清除超氧阴离子IC50分别为6.94和3.68mg/L,表明花色苷还原能力强,并能有效地清除DPPH自由基和超氧阴离子.此外,紫甘薯花色苷能较好地抑制脂质过氧化.

  14. Correlation relationship betw een sexual reproduction and soil nutrient of invasive species Ipomoea cairica%入侵植物五爪金龙有性生殖与土壤养分关系的研究

    Institute of Scientific and Technical Information of China (English)

    陈丹生; 马瑞君; 王桔红

    2015-01-01

    By using statistical data of number of flowers and seeds , seed rate , soil nutrient in the 10 populations of Ipomoeacairica ,the correlation between sexual reproduction and soil nutrient of invasive species Ipomoea cairica was searched by regression analysis method . The result showed that number of flowers was moderate negatively related to soil organic matter (SOM ,r= -0•594);number of seeds inversely related to soil organic matter (r= -0•801) , soil water content (r= -0•656) and available nitrogen (AN )/available phosphorus (AP ,r= -0•665);seed rate and AN , SOM , AN/AP have the inverse correlation relations obviously . Based on the above , the conclusion can be drew that under higher soil nutrient contents condition , I•cairica tend to nutritive grow th and clonal propagation , w hen soil nutrient deficiency , the plant will allocate greater resources to sexual reproduction so that obtain more opportunity for future invasion .%统计了10个不同生境的五爪金龙种群开花数量、结种数量以及结实率,测定了生境中土壤养分中的速效氮、速效磷、速效钾等指标,通过回归分析探讨了五爪金龙有性生殖与土壤养分的关系。结果显示:五爪金龙开花数量仅与土壤养分中有机质含量呈中度负相关;结种数量与有机质含量呈高度负相关,与土壤含水量、氮/磷呈中度负相关;结种率与速效氮、有机质、土壤含水量和氮/磷等多项指标呈中度负相关,其中,与速效氮和氮/磷相关程度达到极显著水平。发现当土壤养分条件优越时,特别是氮/磷比值大、有机质含量高时,更有利于五爪金龙的营养生长和无性繁殖;当土壤养分条件不良时,植物则将资源更多地投入有性生殖,产生种子,以获得更好的入侵机会。

  15. Effects of Simulated Acid Rain on Photosynthetic Physiology Characteristics of Ipomoea Cairica Seedling%模拟酸雨对五爪金龙幼苗光合生理特性的影响

    Institute of Scientific and Technical Information of China (English)

    赵则海

    2014-01-01

    The inhibition of acid rain on growth and development of crop and economic crop often caused serious economic lossess in the Southern China. However, some harmful invasive species was still spreading and its harmfulness was not obviously inhibited under acid rain conditions. Therefore, the study on biological characteristics of invasive species with acid rain condition will help reveal its invasion rules. The pot experiments for Ipomoea cairica seedling were implemented under artificial environmental conditions (light incubator conditions:temperature was 30℃, relative humidity was 47.6%and photosynthetic active radiation (PAR) was 63.6 μmol·m-2·s-1). And nutrient solution was spraying on leaves of I. cairica seedling every day. I. cairica seedling were continuously treated with simulated acid rain (pH value of acid rain were 2, 3, 4, 5 and 6). The photosynthetic physiology characteristics were mensurated using LI-6400 portable photosynthesis system and spectrophotometry. The results showed that:the net photosynthetic rate of leaves of I. cairica decreased with reducing the pH values of acid rain. When the photosynthetic active radiation was less than 200 μmol·m-2·s-1, the net photosynthetic rate increased with increasing of photosynthetic active radiation under different acid rain treatments. When the photosynthetic active radiation was more than 200 μmol·m-2·s-1, rules of the net photosynthetic rates with different pH values of acid rain at the same photosynthetic active radiation were as follows: CK(control check)> pH6.0> pH5.0> pH4.0 > pH3.0> pH2.0. With decreasing of pH values of acid rain, light saturation point (LSP), light compensation point (LCP), maximum net photosynthetic rate, apparent quantum yield (AQY) and dark respiration rate displayed a decrease trend. Among them, the inhibitory effects on all photosynthetic characteristic parameters reached the significant level under the acid rain treatments of pH3.0 and pH2.0. The treatments of acid

  16. STUDIES ON ION BEAM APPLICATION TO IMPROVE AQUATIC MACROPHYTE REMEDIATION CAPACITY IN EUTROPHIC WATERS%利用离子束提高水生植物对富营养化水体修复功能的初步研究

    Institute of Scientific and Technical Information of China (English)

    LI Miao; WU Yue-Jin; ZHANG Jun; YU Han-Qing; WU Xiao-Lei; YU Zeng-Liang

    2006-01-01

    Using ion beam biotechnology in combination with soil-less plant cultivation on artificial substratum (floating beds), the experiments were conducted with Ipomoea aquatica Forsk. Plants were attached to floating-beds which were placed on the surface of artificially nutrient-enriched tank water, in order to study the purification and remediation efficiency of ion beam-treated I.aquatica cultivars. The results show that N+ ion beams with 25keV energy and dosages of 0, 2.6, 3.9, 5.2, 6.5, 7.8, 9.1 ×1013N+ (ions)/cm2 affected I. aquatica dry seeds differently, with the dose of 3.9 × 1013N+ (ions)/cm2 improving effectively the performance as expressed by various biological indices. After ion beam application, I. aquatica cultivars grew well in nutrient-enriched water bodies, increasing the growth of leaves and stem, number of leaves, length and area of roots, plant height, and weight more remarkably than observed in the control. The net removing rates of TN, TP were as high as 75% and 82%, respectively. Especially under the dose of 3.9 × 1013N + (ions)/cm2, the net removing rates of TN, TP were highest, for 77% and 85%, respectively. It was proved that ion beam application improves phytoremediation and may be used to purify nutrient rich water bodies.

  17. Biomonitoring of selected freshwater macrophytes to assess lake trace element contamination: a case study of Nal Sarovar Bird Sanctuary, Gujarat, India

    Directory of Open Access Journals (Sweden)

    Rita N. KUMAR

    2006-02-01

    Full Text Available A biomonitoring study was carried out at Nal Sarovar Bird Sanctuary, a proposed Ramsar site, Gujarat State, India, to ascertain the degree of trace element contamination. The study focused on assessment of trace element contamination in certain aquatic macrophytes to be used as biomonitors, in comparison with the sediments (abiotic monitor for heavy metal pollution. Good information was provided by analyzing roots, stems and leaves of native aquatic plants (biomonitors represented by eight species: Bergia odorata, Hydrilla verticillata, Ipomoea aquatica, Najas graminea, Nelumbo nucifera, Phragmites karka, Typha angustata and Vellisnaria spiralis, alongwith surface sediments and water, were analyzed for Cd, Co, Cu, Cu, Ni, Pb and Zn contamination. The highest concentrations of the trace elements were measured in Ipomoea aquatica and the lowest in Bergia odorata. Based on the concentration and toxicity status observed in the lake's vegetation, the six metals are arranged in the following decreasing order: Zn > Cu > Ni > Co > Pb > Cd. Compared with the standard, normal and critical toxicity range in plants, the detected values of Cd and Pb falls within normal range, while that of Co, Ni and Cu were within the critical range. However, Zn showed the highest concentration and alarming toxicity levels, which is considered as one of the most hazardous pollutants in Nal Sarovar Bird Sanctuary. Certain aquatic macrophytes species are also proposed as biomonitors for the investigated heavy metal pollutants. Such result was significant in the plant species such as Ipomoea aquatica and Phragmites karka, which are the two most useful species in biomonitoring studies due to their ability to accumulate elements in high concentration in the roots and their availability throughout the year. The results showed the significant difference in accumulation rate of some metals like Zn, Cu and Ni in different plant organs, which showed more accumulation in root than

  18. 水生蔬菜种质资源野外考察收集方法

    Institute of Scientific and Technical Information of China (English)

    柯卫东; 刘义满; 李峰; 黄新芳; 彭静

    2007-01-01

    水生蔬菜主要指淡水中生长的维管束植物,包括莲藕(Nelumbo nucifera Gaertn.)、茭白[Zizania caduciflora(Turez.)Hand.-Mazz.]、芋[Colocasia esculenta(L.)Schott]、水芹(Oenanthe spp.)、慈姑(Sagittaria spp.)、荸荠[Eleocharis tuberosa(Roxb.)Roem.et Schult.]、蕹菜(Ipomoea aquatica Forsk.)、芡实(Euryale ferox Salisb.)、

  19. STUDY OF AQUATIC ANGIOSPERMIC PLANTS OF ANAND CITY, GUJARAT, INDIA

    Directory of Open Access Journals (Sweden)

    K. R. PATEL1 AND N. K. PATEL2

    2014-06-01

    Full Text Available The present study deals with the taxonomic study of Aquatic Angiosperms growing throughout the Anand city. The plants are listed along with their brief taxonomic account of each species with current nomenclature, vernacular name, family and uses. The  collected plants are systematically observed during present work, During my study I observed various aquatic angiospermic plants such as   Ceratophyllum demersum, Colocasia esculenta, Eichhornia crassipes, Ipomoea aquatica, Nymphoides indicum, Ludwigia repens, Polygonum orientale, Typha elephantina, Lemna perpusilla, Spirodella polyrrhiza, Xanthium indicum, Phyllanthus reticulatus, Cynodon dactylon, Hydrilla verticillata were very common. Whereas Nymphaea nouchali, Polygonum barbatum, Scirpus articulatus were very rare in the study area.

  20. Utilization effect of cassava (Manihot esculenta and potatoe (Ipomoea batatas on pigmentation, carotenoids pull on egg yolk and performance yield hens Influencia de harinas de yuca y de batata sobre pigmentación, contenido de carotenoides en la yema y desempeño productivo de aves en postura

    Directory of Open Access Journals (Sweden)

    Ceballos L Hernán

    2006-09-01

    Full Text Available

    Utilization effect of cassava (Manihot esculenta and potatoe (Ipomoea batatas on pigmentation, carotenoids pull on egg yolk and performance yield hens. Eight diets (T1:without pigment, T2: commercial pigment, T3: cassava meal 15%, T4: cassava meal 30%, T5: sweet potato meal 15%, T6: sweet potato meal 30%, T7: cassava and sweet potato meal 15% each, T8: cassava and sweet potato meal 25% each in a randomized design using four replicates of three hens each during seven experimental periods were evaluated. Diets were iso-energetics (2.9 kcal/g and iso-proteis (14.5% CP. Carotenoid concentrations (UV-visible chromatography had differences (P<0.01 among diets. Pigmentation (Fan Roche and carotenoid concentration in egg yoks to come from cassava and sweet potato diets did not show differences (P>0.05 A comparison with egg yolk to come from test diets significant differences (P<0.01 were detected. Diet with commercial pigment showed the highest pigmentation and carotenoid concentration in egg yolk. The other hand, there saw not differences (P>0.05. A comparison with egg yolk to come from test diets significant differences (P<0.01 were detected. Diet with commercial pigment showed the highest pigmentation and carotenoid concentration in egg yolk. The other hand, there saw not differences (P>0.05 between egg yolk to come from diet without pigment and cassava and sweet potato diets. Lutein, -carotene, -carotene, and 9-cis-- carotene were the

  1. A comparison of arsenic mobility in Phaseolus vulgaris, Mentha aquatica, and Pteris cretica rhizosphere

    National Research Council Canada - National Science Library

    Száková, Jiřina; Tlustoš, Pavel; Goessler, Walter; Findenig, Silvia; Richtrová, Eva; Balík, Jiří

    2009-01-01

    .... However, there were observed differences in the distribution of the mobile As portion in soil to individual As species as affected by plant species and/or plantation conditions of these plants...

  2. 农药CuSO4处理对空心菜生长及营养品质的影响%The Effects of Pesticide CuSO4 on the Growth,Nutrition and Quality of Ipomoea aquatic

    Institute of Scientific and Technical Information of China (English)

    王瑞聪; 杨颜颜; 孙林华; 杜建梅; 葛峰; 陆长梅

    2015-01-01

    With the increase of focus on food safety,copper agents which have the characteristics of pollution-free,no re⁃sistance etc. are widely used in organic agricultural production. Whether the extensive application of copper agents will bring influence on the yield and quality of foods or not is noteworthy. To inspect this influence,the changes of seed ger⁃mination,seedling growth and vegetable quality of Ipomoea aquatic under CuSO4 processing will be assayed in this pa⁃per.Results showed that CuSO4 treatment not over 1 mM had no obvious effect on seed germination rate,but would re⁃duce seed germination quality. CuSO4 treatment not over 9 mM had no obvious effect on seedling growth,photosynthesis, yield and nutrient content. CuSO4 treatment would increase copper accumulation in I. aquatic seedlings,and copper would accumulate preferentially in roots. Copper content in leaves and stems in the treated I. aquatic seedlings would not exceed the national standard,but it would exceed the standard in roots when CuSO4 was not less than 3 mM. CuSO4 treatment would reduce the nitrate reductase activities which led to the nitrate accumulation to the severe pollution level in I. aquatic seedlings. This would reduce the vegetable quality and enhance the human cancer risk.%随着人们对食品安全关注度的增加,具有无公害、不产生抗药性等特点的铜制剂正被大量应用于有机农业生产中。铜制剂的大量施用是否真对食品品质无害值得关注。本文以空心菜的种子和幼苗为材料,分析了CuSO4处理下种子萌发和幼苗生长、品质等的变化。结果显示,≤1 mM CuSO4的处理对种子萌发率影响不明显,但会降低种子萌发质量;≤9 mM CuSO4的处理对空心菜幼苗的生长、光合、产量和营养含量影响不大;CuSO4处理会增加空心菜体内的铜积累,并将铜优先积累于根部;各处理组空心菜茎叶铜含量不超标,但≥3 mM CuSO4处理将会导致

  3. Diversity analysis of sweet potato (Ipomoea batatas [L.] Lam ...

    African Journals Online (AJOL)

    aghomotsegin

    2014-02-05

    Feb 5, 2014 ... leaf size; ALVP, abaxial leaf vein pigmentation; PL, petiole length; PP, petiole ..... Number of observation =109, Variables in the analysis=30, Class level=3, Significance level to ..... (2008) in Brazil, in Taiwan by Shih et al.

  4. Agronomic performance of locally adapted sweet potato (Ipomoea ...

    African Journals Online (AJOL)

    SERVER

    limited fertility and inadequate moisture (Bioethics, 2004). Despite sweet potato having a high ... the susceptibility of sweet potato to virus diseases which has caused substantial yield reduction of up to 80%. (Karyeija et al., 2000; Odame et al., ...

  5. evaluation of the performance of improved sweet potato (ipomoea

    African Journals Online (AJOL)

    IKUMOINEIN W. KURO

    Department of Crop Production Technology, Niger Delta University, Bayelsa State, ... sing to make alcohol and starch (Martins and Leonard, ... sometimes, the young leaves and shoots are eaten fresh. ..... Academic Press Inc; New York. pp.

  6. Drilling Mud Formulation Using Potato Starch(Ipomoea Batatas

    Directory of Open Access Journals (Sweden)

    WamiEmenikeNyeche

    2015-09-01

    Full Text Available In the search for suitable local alternatives as additives in the manufacture of drilling muds which is an essential entity in the exploration of oil and gas, some vital considerations must be put in place such as cost and environmental effect. This study focuses on the suitability of locally processed potato starch as a viscosifier and fluid loss agent in drilling mud. Comparative analysis of properties obtained from the prepared potato starch mud and that formulated from Polyanionic cellulose (PAC were carried out. Results from this investigation showed that rheological properties (plastic viscosity and yield point of the potato starch mud increased when the content of both viscosifiers were equal at 1.0g/ 316.4ml of water. Plastic viscosity also increased by 13.6% when potato starch concentration increased by 50%. Also, a combination of PAC and potato starch at a ratio of 1:1 to 0.5:1.5 gave a fluid loss of 7.1 - 7.7 ml which were very close to that of the standard mud at 6.8ml. the pH, mud weight and specific gravity of the formulated mud samples ranged from 7.0 - 9.0, 7.0 - 9.1 and 0.83 - 1.09 respectively, which were all in line with the standard mud specifications.

  7. Susceptibility of sweetpotato (Ipomoea batatas) peel proteins to digestive enzymes

    Science.gov (United States)

    Sweet potato proteins have been shown to possess antioxidant and antidiabetic properties in vivo. The ability of a protein to exhibit systemic effects is somewhat unusual as proteins are typically susceptible to digestive enzymes. This study was undertaken to better understand how digestive enzymes ...

  8. Effect of fermentation on Sweetpotato (Ipomoea batatas) toxicity in mice.

    Science.gov (United States)

    Thibodeau, Michael S; Poore, Matthew H; Hagler, Winston M; Rogers, Glenn M

    2004-01-28

    Unfortunate bovine fatalities occurring after ingestion of mold-damaged sweetpotatoes preclude the use of the culled tubers in livestock feed. In cattle, mold-damaged sweetpotatoes induce an acute respiratory distress syndrome resulting in asphyxiation. Because of this potential toxicity and the general abundance of culled sweetpotatoes, the detoxification efficacy of ensiling was explored since it is an easy and economically viable technique often applied to preserve livestock feed. Sweetpotato slices with or without mold damage were stored either frozen (to represent unfermented samples) or fermented for 6 weeks at room temperature. Following fermentation, organic extracts were generated for administration to mice. Thirty hours following administration of the extracts, mice were evaluated for gross and microscopic lesions affecting the lungs, liver, and kidneys. Fermentation of 6 weeks duration was observed to inadequately eliminate the lung, liver, and kidney toxicity caused by mold-damaged sweetpotatoes. In fact, fermentation exacerbated the hepatotoxicity of mold-damaged sweetpotatoes. This is also the first demonstration that sweetpotato regions lacking visible mold damage can induce lung and kidney injury, which, however, is preventable by fermentation.

  9. Aqueous Extract of Ipomoea batatas Reduces Food Intake in Male ...

    African Journals Online (AJOL)

    and have high energy density. Most studies of ... fiber decreases the energy density of food; some fibers such as guar gum .... Lab and Life Science San Diego, California, United States). .... Slavin J, Green H. Dietary fibre and satiety. Nutr Bull.

  10. Immobilisation of Cu, Pb and Zn in Scrap Metal Yard Soil Using Selected Waste Materials.

    Science.gov (United States)

    Kamari, A; Putra, W P; Yusoff, S N M; Ishak, C F; Hashim, N; Mohamed, A; Isa, I M; Bakar, S A

    2015-12-01

    Immobilisation of heavy metals in a 30-year old active scrap metal yard soil using three waste materials, namely coconut tree sawdust (CTS), sugarcane bagasse (SB) and eggshell (ES) was investigated. The contaminated soil was amended with amendments at application rates of 0 %, 1 % and 3 % (w/w). The effects of amendments on metal accumulation in water spinach (Ipomoea aquatica) and soil metal bioavailability were studied in a pot experiment. All amendments increased biomass yield and reduced metal accumulation in the plant shoots. The bioconcentration factor and translocation factor values of the metals were in the order of Zn > Cu > Pb. The addition of ES, an alternative source of calcium carbonate (CaCO3), has significantly increased soil pH and resulted in marked reduction in soil metal bioavailability. Therefore, CTS, SB and ES are promising low-cost immobilising agents to restore metal contaminated land.

  11. Efektivitas Bioremediasi Lima Jenis Tanaman Terhadap Kandungan Logam Berat (Cr2+dan Pb2+ dalam Air

    Directory of Open Access Journals (Sweden)

    I.G. Seregeg

    2012-09-01

    Full Text Available Telah dilakukan penelitian di Laboratorium, tentang efektivitas bioremediasi lima jenis tanaman ; mendong {Scirpus articularis welingi (Cyperus sp, purun (Typha sp., tales-talesan (Typhonium sp, kangkung (Ipomoea aquatica terhadap kandungan Cr2+ dan Pb2+ dalam air tercemar di Jakarta, Desember 1994. Kelima jenis tanaman tersebut ditempatkan dalam akuarium berkapasitas 10 liter, yang diisi air tercemar Cr2+ dan Pb2+ sebanyak lima liter. Penelitian ini dilengkapi dengan akuarium kontrol, seluruh perlakuan diulang dua kali. Hasilnya menunjukkan, bahwa semua jenis tanaman mempunyai kemampuan yang berbeda-beda. Kemampuan yang paling kuat ditunjukkan oleh purun disusul berturut-turut oleh welingi, mendong, kangkung dan tales-talesan. Pengaruh tanam terhadap Pb2+ menunjukkan, daya serap yang cukup kuat dan pola urutannya sama seperti yang ditunjukkan terhadap Cr2+. Tanaman yang tumbuh tegak dengan batang yang kuat (rumput-rumputan mempunyai daya serap yang lebih tinggi dibandingkan dengan tanaman menjalar.

  12. 阴沟肠杆菌对乐果的降解效果试验初探%Preliminary Study on the Degradation Characteristics of Dimethoate by Enterobacter closcae

    Institute of Scientific and Technical Information of China (English)

    马丽娜

    2011-01-01

    The degradation efficiency of dimethoate residue on water spinach(Ipomoea aquatica Forsk) by Enterobacter closcae was studied.The results showed that,the half-life of degradation of dimethoate residue on water spinach reduced to 2.69 d and the degrading rate was 47.30% at 14 d after application of fermentation liquid of E.closcae.%研究了阴沟肠杆菌对空心菜上乐果残留的降解效果,结果表明:施用阴沟肠杆菌培养液后,乐果在空心菜上的降解半衰期减少至2.69 d,施药14 d后的相对降解率为47.3%。

  13. Water Hyacinth Eichhornia crassipes (Mart. Solms-Laubach Dynamics and Succession in the Nyanza Gulf of Lake Victoria (East Africa: Implications for Water Quality and Biodiversity Conservation

    Directory of Open Access Journals (Sweden)

    John Gichuki

    2012-01-01

    Full Text Available This study, conducted in Nyanza Gulf of Lake Victoria, assessed ecological succession and dynamic status of water hyacinth. Results show that water hyacinth is the genesis of macrophyte succession. On establishment, water hyacinth mats are first invaded by native emergent macrophytes, Ipomoea aquatica Forsk., and Enydra fluctuans Lour., during early stages of succession. This is followed by hippo grass Vossia cuspidata (Roxb. Griff. in mid- and late stages whose population peaks during climax stages of succession with concomitant decrease in water hyacinth biomass. Hippo grass depends on water hyacinth for buoyancy, anchorage, and nutrients. The study concludes that macrophyte succession alters aquatic biodiversity and that, since water hyacinth infestation and attendant succession are a symptom of broader watershed management and pollution problems, aquatic macrophyte control should include reduction of nutrient loads and implementing multifaceted approach that incorporates biological agents, mechanical/manual control with utilization of harvested weed for cottage industry by local communities.

  14. 五爪金龙、南美蟛蜞菊入侵对土壤化学和微生物学性质的影响%Impacts of Ipomoea cairica and Wedelia trilobata invasion on soil chemical and microbiological characteristics

    Institute of Scientific and Technical Information of China (English)

    全国明; 毛丹鹃; 章家恩; 谢俊芳; 秦钟

    2016-01-01

    【目的】五爪金龙( Ipomoea cairica)、南美蟛蜞菊( Wedelia trilobata)已入侵我国华南地区并造成严重危害。本文研究了五爪金龙入侵群落、南美蟛蜞菊入侵群落与土著植物类芦( Neyraudia reynaudiana)群落内的土壤养分、土壤微生物量、土壤酶活性以及微生物群落代谢活性、碳源利用特征与功能多样性的变化规律,探讨两种外来植物入侵对土壤生态系统的影响,为揭示其野外入侵机制提供科学依据。【方法】采用野外样方法于2010年1月在广州市东郊的火炉山森林公园设置五爪金龙入侵区、南美蟛蜞菊入侵区和土著植物类芦区3类样地,测定土壤养分、土壤酶活性、土壤微生物量与微生物功能多样性。【结果】1)与土著植物区相比,五爪金龙入侵区土壤有机碳、全氮、全磷以及速效氮、速效磷、速效钾含量显著提高,增幅达到60.38%230.01%;南美蟛蜞菊入侵区土壤有机碳、全氮、速效氮、速效磷含量亦显著提高,增幅达到50.54%145.52%;两种外来植物入侵区土壤C/N比显著降低,但对全钾含量的影响不明显。2)两种外来植物能够显著提高入侵地的土壤微生物量,其中五爪金龙、南美蟛蜞菊入侵区的土壤微生物量碳( Cmic )、氮( Nmic )、磷( Pmic )含量分别比土著植物区增加105.00%152.15%和61.51%138.27%,但土壤微生物量在两个入侵区之间的差异不明显;对于土壤微生物熵,南美蟛蜞菊入侵区的Cmic/Corg值显著高于土著植物区,但Nmic/Nt、 Pmic/Pt 值3类样地之间差异不显著。3)两种外来植物入侵显著提高土壤脲酶、蛋白酶、蔗糖酶和纤维素酶的活性,其中南美蟛蜞菊入侵区的土壤脲酶、蛋白酶和纤维素酶活性最高,分别比土著植物区增加70.35%、21.51%和227.86%;对于蔗糖酶活性,五爪金龙、南美

  15. Nutrient removal of a floating plant system receiving low- pollution wastewater: Effects of plant species and influent concentration

    Science.gov (United States)

    Duan, J. J.; Zhao, J. N.; Xue, L. H.; Yang, L. Z.

    2016-08-01

    Plant floating bed was adopted in this study to compare the purification effect of four plant species (Oenanthe javanica, Ipomoea aquatica, Hydrocotyle vulgaris, and Iris sibirica) receiving high and low treated domestic sewage. The experiment was conducted for eight months during the low temperature season. The results indicated that the average removal rates of TN and NH4+-N in I. aquatica floating bed were relatively high both under high and low influent concentration during the first stage of the experiment. During the second stage, H. vulgaris showed the best performance for nitrogen treatment, and the average removal rates of TN were 70.7% and 87.7% under high and low influent concentration, while the average removal rates of NH4 +-N were as high as 98.9% and 98.9%, accordingly. Moreover, H. vulgaris contributed most for plant assimilation to nitrogen removal among different plant floating systems. It was also found that the existence of hydrophytes effectively controlled the rise of water pH value and algae growth and reproduction, which helped to improve the aquatic environment. The results provide engineering parameters for the future design of an ecological remediation technology for low-pollution wastewater purification.

  16. Efficiency of removing nitrogen and phosphorous and effects on extracellular enzyme activity in water body by five hydrophytes%5种水生植物的脱氮除磷效果及其对水体胞外酶活的影响

    Institute of Scientific and Technical Information of China (English)

    汪文强; 王子芳; 高明

    2016-01-01

    To investigate the role of hydrophytes in the purification of eutrophic water bodies,Trapa quadrispinosa Roxb.,Eichhornia crassipes,Hydrocharis dubia (Bl.) Backer,Ipomoea aquatica Forsk,and Lemna minor L.were planted in treated water to compare their effects on purification and their influence on extracellular enzyme activity.The results showed that all hydrophytes performed well during the purification process.For total nitrogen (TN),the order of removal efficiency was Eichhornia crassipes (81.6%) > Ipomoea aquatica Forsk (72.2%) > Hydrocharis dubia (Bl.) Backer (68.4%) > Trapa quadrispinosa Roxb.(65.8%) > Lemna minor L.(60.2%),and for total phosphorous (TP) removal,the order was Eichhornia crassipes (95.0%) > Ipomoea aquatica Forsk (89.7%) > Hydrocharis dubia (Bl.) Backer (78.4%) > Trapa quadrispinosa Roxb.(77.3%) > Lemna minor L.(70.5%).All five hydrophytes could enhance the activity of extracellular enzymes,and the treatment involving E.crassipes showed the maximum range and peak urease activity (UA) and alkaline phosphatase activity (APA).The range and peak UA were 19.70 μg · mL-1 and 10.33 μg · mL-1,respectively,and for APA,they were 8.12 μg · mL-1 and 5.95 μg · mL-1.The range and peak UA and APA for Ipomoea aquatica Forsk were the next highest.UA and APA were significantly negatively correlated with the concentrations of both NH4-N and dissolved active phosphorus (SRP),and the correlations were between-0.853 and-0.992 and -0.813 and-0.994,respectively.Eichhornia crassipes and Ipomoea aquatica Forsk may work better than Trapa quadrispinosa Roxb,Hydrocharis dubia (Bl.) Backer and Lemna minor L.,and have potential for application in the purification of eutrophic water bodies.%利用静态小区实验研究了菱角(Trapa quadrispinosa Roxb.)、凤眼莲(Eichhornia crassipes.)、水鳖(Hydrocharis dubia (Bl.) Backer)、空心菜(Ipomoea aquatica Forsk)和青萍(Lemna minor L.)5种不同植物对富营养水体的净

  17. [Treatment effect of biological filtration and vegetable floating-bed combined system on greenhouse turtle breeding wastewater].

    Science.gov (United States)

    Chen, Chong-Jun; Zhang, Rui; Xiang, Kun; Wu, Wei-Xiang

    2014-08-01

    Unorganized discharge of greenhouse turtle breeding wastewater has brought several negative influences on the ecological environment in the rural area of Yangtze River Delta. Biological filtration and vegetable floating-bed combined system is a potential ecological method for greenhouse turtle breeding wastewater treatment. In order to explore the feasibility of this system and evaluate the contribution of vegetable uptake of nitrogen (N) and phosphorus (P) in treating greenhouse turtle breeding wastewater, three types of vegetables, including Ipomoea aquatica, lettuce and celery were selected in this study. Results showed the combined system had a high capacity in simultaneous removal of organic matter, N and P. The removal efficiencies of COD, NH4(+)-N, TN and TP from the wastewater reached up to 93.2%-95.6%, 97.2%-99.6%, 73.9%-93.1% and 74.9%-90.0%, respectively. System with I. aquatica had the highest efficiencies in N and P removal, followed by lettuce and celery. However, plant uptake was not the primary pathway for TN arid TP removal in the combined system. The vegetable uptake of N and P accounted for only 9.1%-25.0% of TN and TP removal from the wastewater while the effect of microorganisms would be dominant for N and P removal. In addition, the highest amounts of N and P uptake in I. aquatica were closely related with the biomass of plant. Results from the study indicated that the biological filtration and vegetable floating-bed combined system was an effective approach to treating greenhouse turtle breeding wastewater in China.

  18. Importance of Macrophyte Quality in Determining Life-History Traits of the Apple Snails Pomacea canaliculata: Implications for Bottom-Up Management of an Invasive Herbivorous Pest in Constructed Wetlands.

    Science.gov (United States)

    Yam, Rita S W; Fan, Yen-Tzu; Wang, Tzu-Ting

    2016-02-24

    Pomacea canaliculata (Ampullariidae) has extensively invaded most Asian constructed wetlands and its massive herbivory of macrophytes has become a major cause of ecosystem dysfunctioning of these restored habitats. We conducted non-choice laboratory feeding experiments of P. canaliculata using five common macrophyte species in constructed wetlands including Ipomoea aquatica, Commelina communis, Nymphoides coreana, Acorus calamus and Phragmites australis. Effects of macrophytes on snail feeding, growth and fecundity responses were evaluated. Results indicated that P. canaliculata reared on Ipomoea had the highest feeding and growth rates with highest reproductive output, but all individuals fed with Phragmites showed lowest feeding rates and little growth with poorest reproductive output. Plant N and P contents were important for enhancing palatability, supporting growth and offspring quantity of P. canaliculata, whilst toughness, cellulose and phenolics had critically deterrent effects on various life-history traits. Although snail offspring quality was generally consistent regardless of maternal feeding conditions, the reduced growth and offspring quantity of the poorly-fed snails in constructed wetlands dominated by the less-palatable macrophytes could limit the invasive success of P. canaliculata. Effective bottom-up control of P. canaliculata in constructed wetlands should involve selective planting strategy using macrophytes with low nutrient and high toughness, cellulose and phenolic contents.

  19. Importance of Macrophyte Quality in Determining Life-History Traits of the Apple Snails Pomacea canaliculata: Implications for Bottom-Up Management of an Invasive Herbivorous Pest in Constructed Wetlands

    Directory of Open Access Journals (Sweden)

    Rita S. W. Yam

    2016-02-01

    Full Text Available Pomacea canaliculata (Ampullariidae has extensively invaded most Asian constructed wetlands and its massive herbivory of macrophytes has become a major cause of ecosystem dysfunctioning of these restored habitats. We conducted non-choice laboratory feeding experiments of P. canaliculata using five common macrophyte species in constructed wetlands including Ipomoea aquatica, Commelina communis, Nymphoides coreana, Acorus calamus and Phragmites australis. Effects of macrophytes on snail feeding, growth and fecundity responses were evaluated. Results indicated that P. canaliculata reared on Ipomoea had the highest feeding and growth rates with highest reproductive output, but all individuals fed with Phragmites showed lowest feeding rates and little growth with poorest reproductive output. Plant N and P contents were important for enhancing palatability, supporting growth and offspring quantity of P. canaliculata, whilst toughness, cellulose and phenolics had critically deterrent effects on various life-history traits. Although snail offspring quality was generally consistent regardless of maternal feeding conditions, the reduced growth and offspring quantity of the poorly-fed snails in constructed wetlands dominated by the less-palatable macrophytes could limit the invasive success of P. canaliculata. Effective bottom-up control of P. canaliculata in constructed wetlands should involve selective planting strategy using macrophytes with low nutrient and high toughness, cellulose and phenolic contents.

  20. CARACTERISATION CHIMIQUE ET ACTIVITES BIOLOGIQUES DES VOLATILS DE Mentha aquaticaL. (DOMRANE) DE L’OUEST ALGERIEN.

    OpenAIRE

    BENOMARI, Fatima Zahra

    2014-01-01

    De part sa situation géographique, sa végétation, son climat, son relief et sa structure, la forêt Algérienne est typiquement méditerranéenne. Elle présente une grande affinité avec les autres forêts méditerranéennes notamment dans sa structure et sa dynamique. Sa flore est réputée pour sa diversité et son endémisme. L’étude floristique de l’ouest Algérien reste relativement satisfaisante, et plus particulièrement les Monts de Tlemcen qui dans leurs ensemble offrent des paysag...

  1. Biogas production from Eichhornia crassipes aquatic plant; Producao de biogas a partir da planta aquatica Eichhornia crassipes

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, Roberto Guimaraes; Silva, Jose Goncalves da; Fernandes Filho, Jorge; Pereira, Maria Cristina Duarte Eiras [Universidade Federal Fluminense (UFF), Niteroi, RJ (Brazil). Dept. de Engenharia Mecanica. Programa de Pos-Graduacao em Engenharia Mecanica]. E-mail: temrobe@vm.uff.br; Melo, Ricardo Bichara de [Light Servicos de Eletricidade S.A., Rio de Janeiro, RJ (Brazil)]. E-mail: rbmelo@light.com.br

    2004-07-01

    Virtually all plants and waste plants and animals may in some way be used as an energy source. The anaerobic digestion of these materials is an option, resulting in the biogas. Besides the gas obtained in the process, is produced, inside the biodigester, an excellent fertilizer. The aquatic plant Eichhornia crassipes is found in large quantities in various water bodies, such as reservoirs, lakes and ponds, becoming mostly often a big problem and it is necessary its systematic removal of water. The bench biodigester used in the experiment of biodigestion of aquatic plants is composed of a reactor containing the biomass, where the biogas is produced, and a reservoir to monitor the production of biogas. The reactor is located within a receptacle containing water that can be heated by an electrical resistance, with the purpose of maintaining the temperature inside the reactor around 35 deg C. The results of analysis of gas of the reactor made in a gas chromatograph to CG MASTER of double ionization detector with a flame and thermal conductivity, show a percentage of 50% of methane in the biogas. The process of biodigestion of aquatic plant Eichhornia crassipes shows potential to obtain biogas, with considerable levels of methane in order to make its exploitation. Also, were analyzed the biomass in the biodigester for determination of humid, total organic matter, mineral and organic carbon residue.

  2. Biodigestion of the aquatics plants mixtures and biogas production; Biodigestao de misturas de plantas aquaticas e producao de biogas

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, Roberto Guimaraes; Abreu, Fernando Luiz Barbuda de; Fernandes Filho, Jorge; Pereira, Maria Cristina Duarte Eiras [Universidade Federal Fluminense (UFF), Niteroi, RJ (Brazil). Dept. de Engenharia Mecanica. Programa de Pos-Graduacao em Engenharia Mecanica]. E-mail: temrobe@vm.uff.br; Melo, Ricardo Bichara de [Light Servicos de Eletricidade S.A., Rio de Janeiro, RJ (Brazil). Gerencia de Estudos e Gestao de Geracao]. E-mail: rbmelo@light.com.br

    2004-07-01

    Several systems of generating electricity using water storage reservoirs. One problem that occurs constantly in these reservoirs is the accumulation of aquatic plants, such as Eichhornia crassipes, Eichhornia azurea, Pistia stratiotes and Salvinia that may cause serious problems for the system. Periodically, the biomass must be removed and disposed of appropriate form, so that does not cause contamination of soil, groundwater or allowing the proliferation of vectors. One possible destination is the use of biomass in a process of biodigestion, resulting in biogas. The bench of biodigester used in the experiment of biodigestion of aquatic plants is composed of a reactor containing the biomass, where the biogas is produced and a reservoir for the monitoring the production of biogas. The reactor is located inside a container containing water that can be heated by an electrical resistance, with the aim of maintaining the temperature inside the reactor around 35 deg C. The results of analysis of gas of the reactor was obtained using a gas chromatograph to CG MASTER of double ionization detector with a flame and thermal conductivity. These results show a percentage of 50% of methane in the biogas. Also, were analyzed the biomass in the biodigester for determination of humidity, total organic matter, waste mineral and organic carbon. The process of biodigestion of the mixture of aquatic plants: Eichhornia crassipes, Eichhornia azurea and Pistia stratiotes and Salvinia shows potential for obtaining biogas, with considerable levels of methane, in order to facilitate its recovery.

  3. PENGARUH PENGOLAHAN PANAS TERHADAP KONSENTRASI ANTOSIANIN MONOMERIK UBI JALAR UNGU (Ipomoea batatas L (Efect of Heat Processing on Monomeric Anthocyanin of Purple Sweet Potato (Ipomoea batatas L

    Directory of Open Access Journals (Sweden)

    Ai Mahmudatussa'adah

    2015-09-01

    content in PSP during heat processing of flakes. The other purpose of this reseach was to observe the order kinetics model of effect temperature and time baking on total anthocyanin monomeric of fresh PSP and rehydration PSP flakes. The experimental applied a completely randomized design with three replications. The color and amount of anthocyanin (L * = 23.38 ± 0.71, C = 9.84 ± 0.98, Hue = 12.25 ± 1.61. Total monomeric anthocyanin in fresh PSP was 1.45 ± 0.00 mg cyanidin equivalent (CyE/g dry basis (db. In general, the color and the amount of PSP anthocyanin changed during the flakes processing. Steamed PSP for 7 minutes turned its color into a bright purple (L * = 25.88 ± 0.47, C = 24.64 ± 0.25, Hue = 348.83 ± 0.33 with the amount of monomeric anthocyanin increased to 3.76 ± 0.01 CyE mg/g db. Flakes PSP was very bright purple (L * = 36.12 ± 0.11, C = 9.97 ± 0.18, Hue = 359.29 ± 0.31 and the amount of monomericanthocyanin was slightly lower than that of steamed sweet potato (3.19 ± 0.12 mg CyE / g db. Total monomeric anthocyanin of fresh PSP and rehydration flakes PSP decrease during baking time. Keywords: Color, anthocyanin, purple sweet potato, flakes, degradation kinetics   ABSTRAK Antosianin merupakan salah satu kelompok zat warna alami yang terdapat pada tanaman, seperti daun, bunga, umbi, buah atau sayur. Salah satu sumber antosianin pada tanaman adalah ubi jalar ungu (UJU yang mengandung lebih dari 98% antosianin terasilasi dari konsentrasi antosianin umbi. Warna antosianin bervariasi mulai dari merah, ungu, biru, sampai kuning. Warna dan konsentrasi antosianin dapat berubah karena pengaruh panas. Penelitian ini bertujuan untuk mempelajari perubahan warna dan konsentrasi antosianin monomerik sebagai akibat proses pengolahan dalam pembuatan  UJU. Penelitian ini mengkaji juga mengenai model kinetika reaksi pengaruh suhu dan waktu panggang terhadap konsentrasi antosianin UJU segar dan  rehidrasi. Penelitian menggunakan rancangan acak lengkap dengan tiga kali ulangan. Warna dan konsentrasi antosianin dari UJU segar, setelah proses pengukusandan setelah menjadi  dibandingkan. UJU segar berwarna ungu kecoklatan (L =23,38±0,71, C=9,84±0,98, Hue=12,25±1,61. Konsentrasi antosianin monomerik pada UJU segar adalah 1,45±0,00 mg setara sianidin (CyE/gbasis kering (bk. Secara umum, warna dan konsentrasi antosianin UJU berubah selama proses pembuatan . UJU yang dikukus selama 7 menit berubah menjadi ungu cerah (L* * =25,88±0,47, C=24,64±0,25, Hue=348,83±0,33 dengan konsentrasi antosianin monomerik meningkat menjadi 3,76±0,01 mg CyE/g bk.  UJU berwarna ungu sangat cerah (L =36,12±0,11, C=9,97±0,18, Hue=359,29±0,31 dan konsentrasi antosianin monomerik sedikit lebih rendah dibandingkan ubi jalar setelah dikusus (3,19±0,12 mg CyE/g bk. Jumlah antosianin monomerik UJU segar dan rehidrasi menurun seiring dengan waktu dan suhu pemanggangan. Kata kunci: Warna, antosianin, ubi jalar ungu, kecepatan penururunan

  4. Research on the Physiological Responses of Six Plants Pennisetum Alopecuroides Etc to BDE-209 in Soil and Their Phytoremediation Effect%狼尾草等6种植物对十溴联苯醚污染土壤的生理响应及其修复效果

    Institute of Scientific and Technical Information of China (English)

    刘京; 尹华; 彭辉; 叶锦韶; 叶芊; 李丽华; 何宝燕

    2012-01-01

    考察了狼尾草、龙葵、空心菜、苣菜、芥菜和鱼腥草6种植物对土壤中十溴联苯醚(BDE-209)的生理响应及其修复效果.结果表明,在BDE-209污染条件下,供试植物体内丙二醛(MDA)含量升高,表明植物受到一定的毒害作用,而在植物抗逆境机制作用下,植物体内可溶性蛋白质含量升高,超氧化物歧化酶(SOD)活性下降;在供试植物生物量方面,BDE-209对龙葵、空心菜和狼尾草地上部有促进作用,而对苣菜、芥菜具有一定的抑制作用;6种修复植物体内均检测出BDE-209,且地上部与根部BDE-209含量具有显著差异,其中狼尾草根部干重含量高达16.93 mg · kg-1;修复60d后,土壤中BDE-209含量均有一定程度的下降,最高去除率可达40.44%,且根际土与非根际土BDE-209去除率有显著差异.修复效果依优劣次序为狼尾草>龙葵、空心菜>鱼腥草>芥菜>苣菜.%The physiological responses of plants Pennisetum alopecuroides, Solarium nigrum, Ipomoea aquatica Forsk, Sonchus brachyotus D C, Brassica juncea and Houttuynia cordata Thunb to contaminant BDE-209 in soil were investigated, and the phytoremediation effects of these plants on BDE-209 contamination were explored. The results indicated that, the contents of MDA in tested plants increased under the condition of BDE-209 pollution, revealing that the plants suffered from toxic effect to a certain extent. Meanwhile the increased soluble protein content and decreased SOD activity were also detected, due to the mechanism of adversity resistance in plants, In respect of biomass of tested plants, BDE-209 promoted the growth of shoots of Pennisetum alopecuroides, Solanum nigrum and Ipomoea aquatica Forsk, but inhibited that of Sonchus brachyotus D C and Brassica juncea to a certain degree. The contents of BDE-209 in all six plants were detected with significant difference existed in shoots and roots. Among these a content of as high as 16.93 mg · kg‐1 BDE

  5. Application of a constructed wetland for industrial wastewater treatment: a pilot-scale study.

    Science.gov (United States)

    Chen, T Y; Kao, C M; Yeh, T Y; Chien, H Y; Chao, A C

    2006-06-01

    The main objective of this study was to examine the efficacy and capacity of using constructed wetlands on industrial pollutant removal. Four parallel pilot-scale modified free water surface (FWS) constructed wetland systems [dimension for each system: 4-m (L)x1-m (W)x1-m (D)] were installed inside an industrial park for conducting the proposed treatability study. The averaged influent contains approximately 170 mg l(-1) chemical oxygen demand (COD), 80 mg l(-1) biochemical oxygen demand (BOD), 90 mg l(-1) suspend solid (SS), and 32 mg l(-1) NH(3)-N. In the plant-selection study, four different wetland plant species including floating plants [Pistia stratiotes L. (P. stratiotes) and Ipomoea aquatica (I. aquatica)] and emergent plants [Phragmites communis L. (P. communis) and Typha orientalis Presl. (T. orientalis)] were evaluated. Results show that only the emergent plant (P. communis) could survive and reproduce with a continuous feed of 0.4m(3)d(-1) of the raw wastewater. Thus, P. communis was used in the subsequent treatment study. Two different control parameters including hydraulic retention time (HRT) (3, 5, and 7d) and media [vesicles ceramic bioballs and small gravels, 1cm in diameter] were examined in the treatment study. Results indicate that the system with a 5-d HRT (feed rate of 0.4m(3)d(-1)) and vesicles ceramic bioballs as the media had the acceptable and optimal pollutant removal efficiency. If operated under conditions of the above parameters, the pilot-plant wetland system can achieve removal of 61% COD, 89% BOD, 81% SS, 35% TP, and 56% NH(3)-N. The treated wastewater meets the current industrial wastewater discharge standards in Taiwan.

  6. Efficacy of Ipomoea batatas (Caiapo) on diabetes control in type 2 diabetic subjects treated with diet.

    Science.gov (United States)

    Ludvik, Bernhard; Neuffer, Beatrice; Pacini, Giovanni

    2004-02-01

    To investigate the tolerability, efficacy, and mode of action of Caiapo, an extract of white sweet potatoes, on metabolic control in type 2 diabetic patients. A total of 61 type 2 diabetic patients treated by diet were given 4 g Caiapo (n = 30; mean age 55.2 +/- 2.1 years; BMI 28.0 +/- 0.4 kg/m(2)) or placebo (n = 31; mean age 55.6 +/- 1.5 years; BMI 27.6 +/- 0.3 kg/m(2)) once daily for 12 weeks. Each subject underwent a 75-g oral glucose tolerance test (OGTT) at baseline and after 1, 2, and 3 months to assess 2-h glucose levels. Additionally, fasting blood glucose, HbA(1c), total cholesterol, and triglyceride levels were measured. After treatment with Caiapo, HbA(1c) decreased significantly (P < 0.001) from 7.21 +/- 0.15 to 6.68 +/- 0.14%, whereas it remained unchanged (P = 0.23) in subjects given placebo (7.04 +/- 0.17 vs. 7.10 +/- 0.19%). Fasting blood glucose levels decreased (P < 0.001) in the Caiapo group (143.7 +/- 1.9 vs. 128.5 +/- 1.7 mg/dl) and did not change in the placebo group (144.3 +/- 1.9 vs. 138.2 +/- 2.1 mg/dl; P = 0.052). A decrease in body weight was observed in both the placebo group (P = 0.0027) and in the Caiapo group (P < 0.0001), probably due to a better- controlled lifestyle. In the Caiapo group, body weight was related to the improvement in glucose control (r = 0.618; P < 0.0002). Two-hour glucose levels were significantly (P < 0.001) decreased in the Caiapo group (193.3 +/- 10.4 vs. 162.8 +/- 8.2 mg/dl) compared with the placebo group (191.7 +/- 9.2 vs. 181.0 +/- 7.1 mg/dl). Mean cholesterol at the end of the treatment was significantly lower in the Caiapo group (214.6 +/- 11.2 mg/dl) than in the placebo group (248.7 +/- 11.2 mg/dl; P < 0.05). No significant changes in triglyceride levels or blood pressure were observed, and Caiapo was well tolerated without significant adverse effects. This study confirms the beneficial effects of Caiapo on plasma glucose as well as cholesterol levels in patients with type 2 diabetes. For the first time, the long-term efficacy of Caiapo on glucose control was demonstrated by the observed decrease in HbA(1c). Thus, the neutraceutical Caiapo seems to be a useful agent in the treatment of type 2 diabetes.

  7. Mercury content of sprouts and harvested roots from treated sweet potato mother roots. [Ipomoea batatas

    Energy Technology Data Exchange (ETDEWEB)

    Huisingh, D.; Nielsen, L.W.

    1972-01-01

    Mercury containing fungicides have been used extensively for seed and root disease control, but data on the fate of the mercury (Hg) are scarce. Experiments were designed to see if Hg applied to propagative sweet potato roots increased the Hg-content of edible roots. Roots were treated with Semesan Bel(hydroxymercurinitrophenol + hydroxymercurichlorophenol), Mertect (Thiabendazole: 2-(4-Thiazolyl)-benzimidazole), or Botran (2,6-Dichloro-4-nitroanaline) at recommended rates or with water. Treated roots were bedded into sandy loam soil, and the plants were harvested at 2 and 3 months after bedding. Some sprouts transplanted at 2 months were grown to maturity, and the harvested roots were analyzed. Hg analyses were performed by flameless atomic absorption. Roots treated prior to planting with Semesan Bel, Mertect, Botran, and water contained 23.0, 0.05, 0.03 and 0.03 ..mu..g/g dry wt, respectively. At the 2-month harvest, the leaves and stems of the Semesan Bel-treated plants contained 5 times more Hg than those of the other treatments. By the 3-month harvest, the amount of Hg in plant leaves and stems from Hg-treated roots was 2 to 3 times that of the others. Fall harvested fleshy roots contained 0.03, 0.02, 0.03, and 0.03 ..mu..g/g dry wt Hg for the Semesan Bel, Mertect, Botran, and water treatments, respectively. This demonstrates that the Hg applied to the mother root was translocated to the new plant, but little if any was translocated to the new fleshy roots.

  8. EVALUATION OF ELASTICITY AND MECHANICAL PROPERTIES OF BREAD DOUGH MADE WITH REPLACED FLOUR POTATO (IPOMOEA BATATA

    Directory of Open Access Journals (Sweden)

    Ely Fernando Sacón Vera

    2016-10-01

    Full Text Available The effect of the incorporation of sweet potato flour, with 30% replacement in 1kg of wheat flour was evaluated to determine the behavior of elastic and mechanical properties during the kneading and baking stage of bread. For the evaluation the following varieties were studied: Morado Brazil, Morado Ecuador, Guayaco Morado, Ina and Toquecita, and the evaluated properties were: texture (hardness, elasticity, firmness, chewiness measured by a texture meter Bloomfield and volume was measured by INEN standard (NTE INEN 0530: 80. The design employed was completely at random, using analysis of variance at 5% significance level. The results obtained showed that elasticity attribute in texture variable presented significant differences (P <0.05. Analysis concluded that including Toquecita flour in the mixture to form the dough, had the highest elasticity of 13.32mm. However, Morado Ecuador variety flour presented a 6.24 mm elasticity value, ideal for both the malleability of the dough and the freshness of the bread, and concerning volume, the inclusion of Ecuador Morado flour and Ina in the formulation of bread, showed an increase in volume at 93.30 and 93.67cm3 respectively, close to the normed value for wheat flour bread.

  9. Isolation of endosymbionts from Ipomoea carnea and Swainsona canescens that produce swainsonine

    Science.gov (United States)

    Fungi including Metarhizium anisopliae (Clavicipitaceae), Rhizoctonia leguminicola (Ceratobasidiaceae), and Undifilum (Pleosporaceae), an endophyte found in the plant genera Astragalus and Oxytropis (Fabaceae) have been reported to be responsible for the production of swainsonine. Based upon the ass...

  10. Therapeutic effects of the traditional medicinal plant Ipomoea stolonifera for the treatment of liver diseases

    NARCIS (Netherlands)

    Bai, Xueting

    2016-01-01

    Liver diseases are categorized into acute liver failure (ALF) and chronic liver failure (CLF). Massive cell death is a hallmark of ALF and leads to a dramatic loss of liver function. Therefore, specific interventions targeted to prevent or attenuate this massive cell death may be very effective in

  11. Quality evaluation of yoghurt stabilized with sweet potato (Ipomoea batatas and taro (Colocassia esculenta starch

    Directory of Open Access Journals (Sweden)

    Aysha Sameen

    2016-08-01

    Full Text Available Stabilizers are important component in manufactured products such as yoghurt. The addition of stabilizers improves body, texture, appearance, mouth feel and prevents technical defects such as synersis in yoghurts. In this study starch was extracted from plant sources (sweet potato, taro with and without use of chemicals. Yoghurt was enriched with different levels of extracted starch. Yoghurt samples were analyzed for physicochemical and functional attributes such as pH, acidity, synersis, water holding capacity, viscosity, total solids and sensory profile. Use of chemically extracted starches at the level of 0.3-0.4% (Sweet potato and 0.2-0.3% (Taro   in yoghurt manufacturing showed better  results  in  terms  of  lowering synersis, increasing water holding capacity, viscosity and overall acceptability as compared to the yoghurt containing stabilizer i.e. gelatin 0.5% w/w. Use of starches did not significantly affect the sensory attributes. Yoghurt that contains sweet potato and taro starch at 0.5% gave excellent results for water holding capacity, viscosity and for all sensory attributes as compared to gelatin

  12. Quality evaluation of yoghurt stabilized with sweet potato (Ipomoea batatas) and taro (Colocassia esculenta) starch

    OpenAIRE

    Aysha Sameen; Muhammad Issa Khan; Muhammad Umair Sattar; Asma Javid; Aimen Ayub

    2016-01-01

    Stabilizers are important component in manufactured products such as yoghurt. The addition of stabilizers improves body, texture, appearance, mouth feel and prevents technical defects such as synersis in yoghurts. In this study starch was extracted from plant sources (sweet potato, taro) with and without use of chemicals. Yoghurt was enriched with different levels of extracted starch. Yoghurt samples were analyzed for physicochemical and functional attributes such as pH, acidity, synersis, wa...

  13. KARAKTERISTIK KIMIA, FISIK DAN INDERAWI TEPUNG UBI JALAR UNGU (Ipomoea batatas Poiret DAN PRODUK OLAHANNYA

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    Ade Krisna Nindyarani

    2012-05-01

    Full Text Available The objectives of the research were to determine the chemical and physical characteristics of purple-flesh sweet potato flour as base of suitability for processed foods production, and to determine the acceptability of products by panelists. Cookies and pound cake were made by using purple-flesh sweet potato flour as raw material and using stages of process as follows: dough preparation, molding, shaping and baking. The results showed that purple-flesh sweet potato flour contain moisture 10.92 ± 0.09 % (db, protein 6.44 ± 0.27 % (db, starch 74.57 ± 0.32 % (db, amylose 24.79 ± 0.94 % (db, reducing sugar 3.15 ± 0.30 % (db, and crude fiber 2.40 ± 0.35 % (db. Peak viscosity at 65 oC was 6.17 dPa.s and setback viscosity was similar to the peak viscosity. Starch granules of purple-flesh sweet potato were  polygonal with size of 10-25 μm. The cookies made by using mixture of 25 % wheat flour and 75 % purple-flesh sweet potato flour, and pound cake made by using 100 % purple-flesh sweet potato flour were acceptable for panelists. Keywords: Purple-flesh sweet potato flour, cookies, pound cake   Abtrak Tujuan penelitian adalah untuk mengetahui karakteristik kimia dan fisik tepung ubi jalar ungu sebagai dasar penentuan kesesuaian untuk pembuatan pangan olahan berbasis tepung ubi jalar ungu serta untuk mengetahui tingkat penerimaan panelis. Tepung ubi jalar ungu diolah menjadi cookies dan pound cake mengikuti tahap penyiapan adonan, pencetakan, dan pemanggangan. Dilakukan uji inderawi cookies dan pound cake melalui uji kesukaan oleh panelis yang  meliputi parameter warna, aroma, cita-rasa, tekstur, dan kesukaan keseluruhan serta uji deskriptif. Hasil penelitian menunjukkan bahwa tepung ubi jalar ungu memiliki berturut-turut komponen kadar air 10,92 ± 0,09 % (bk, protein 6,44 ± 0,27 % (bk, pati 74,57 ± 0,32 % (bk, amilosa 24,79 ± 0,94 % (bk, gula reduksi 3,15 ± 0,30 % (bk, dan serat kasar 2,40 ± 0,35 % (bk. Viskositas puncak tepung ubi jalar ungu pada suhu 65 oC sebesar 6,17 dPa.s, dan viskositas baliknya relatif sama dengan viskositas puncak. Granula pati tepung ubi jalar ungu berbentuk poligonal dengan ukuran 10-25 μm. Cookies yang dibuat dari kombinasi antara tepung terigu 25 % dan tepung ubi jalar ungu 75 %, dan  pound cake yang dibuat dari tepung ubi jalar ungu 100 % (tanpa tepung terigu cukup disukai panelis. Kata kunci: Tepung ubi jalar ungu, cookies, pound cake

  14. and Boiling on Pro-Vitamin A Content in Sweet Potato (Ipomoea

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    To maximize the availability of pro-vitamin A carotenoids in sweet potato and to recommend the appropriate start of piecemeal ..... sufficicnt levels of retinol equivalents to meet this criteria. .... molecular weight fragments associated with further ...

  15. in vitro production of virus free sweet potato [ipomoea batatas (l

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    ABSTRACT: The main objective of this study was to determine the levels of fluoride in cereals. (tef, wheat ... of energy and nutrient needs. Staple foods .... boreholes and 2 to 68 mg/L in geothermal wells. (Tamiru .... 40 filter paper. The pH of the.

  16. Pengaruh Macam dan Waktu Aplikasi Bahan Organik pada Tanaman Ubi Jalar (Ipomoea batatas L. Var. Kawi

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    Nur Edy Suminarti

    2015-07-01

    Full Text Available Diversifikasi pangan merupakan langkah tepat untuk mengantisipasi kondisi rawan pangan.  Hal ini menjadi penting karena setiap tahun luas lahan basah telah mengalami penyusutan sekitar 0,1% dari total luas lahan di Indonesia. Sehubungan dengan hal tersebut, dan dalam upaya untuk memenuhi kebutuhan pangan nasional, maka pemanfaatan ubi jalar sebagai sumber bahan pangan alternatif perlu dilakukan. Penelitian yang bertujuan untuk mendapatkan informasi tentang macam dan waktu aplikasi bahan organik yang tepat telah dilakukan di Desa Landungsari, Kabupaten Malang. Rancangan yang digunakan adalah Petak Terpisah, macam bahan organik ditempatkan pada petak utama, terdiri dari 3 macam, yaitu  pupuk kandang sapi, kompos azolla dan kompos sampah kota.  Waktu aplikasi bahan organik sebagai anak petak, terdiri dari 3 macam, yaitu : 30 hari sebelum tanam, 15 hari sebelum tanam dan bersamaan tanam. Pengumpulan data dilakukan secara destruktif, meliputi  komponen pertumbuhan dan panen, analisis pertumbuhan tanaman dan analisis tanah. Uji F taraf 5% ditujukan untuk menguji pengaruh perlakuan, sedang perbedaan diantara rata-rata perlakuan didasarkan pada nilai BNT taraf 5%. Interaksi nyata terjadi pada sebagian besar parameter yang diamati, dan hasil umbi tertinggi didapatkan pada kompos sampah kota yang waktu aplikasinya dilakukan 30 hari sebelum tanam : 28,03 ton umbi ha-1. Food diversification is a precise step to anticipate food shortage condition. It becomes important because wetland area is decreasing of approximately 0,1% each year of total area in Indonesia. Related to that point, also in order to meet food national demand, utilization of sweet potato as alternative food resource needs to be done. Research aimed to get information about proper kind and application time of organic matter had been done at Landungsari, Malang. The design used was Split Plot, kind of organic matter was placed as main plot, consisting of 3 kinds, ie: cow manure, azzola compost, and urban waste compost. Application time was set as subplot, consisting of 3 times, ie: 30 days before planting, 15 days before planting, and simultaneous with planting. Data collection was carried out destructively, including growth and yield components, plant growth analysis and soil analysis. F test on 5% level intended to test treatment effect, meanwhile difference between mean values based on LSD 5%. Real interaction occurred on almost all parameters observed, moreover the highest tuber yield was on urban waste compost which time application was 30 days before planting : 28,03 ton tuber ha-1.

  17. Evaluación agronómica de trece genotipos de camote (Ipomoea batatas L

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    Rolbin Castillo Matamoros

    2014-12-01

    Full Text Available El objetivo del presente trabajo fue caracterizar desde el punto de vista agronómico, 13 genotipos de camote para su cultivo en Costa Rica. Diez fueron introducidos de la Unidad de Micropropagación de la Universidad Estatal de Carolina del Norte, y cultivados por primera vez en Costa Rica, 2 conocidos como “Exportación y Zanahoriaˮ son de introducción reciente, pero se han cultivado en el país por al menos 5 años y el restante Criollo es el que ocupa actualmente la mayor área de siembra. El trabajo se llevó a cabo en la Estación Experimental Fabio Baudrit de la Universidad de Costa Rica, ubicada en la provincia de Alajuela. Los tratamientos se dispusieron en el campo en un diseño de bloques completos al azar, con 13 genotipos y 4 repeticiones. El análisis de varianza mostró que los genotipos difirieron significativamente (p=0,0001 en todas las variables evaluadas: peso fresco y seco foliar; peso fresco y seco de raíz reservante; número de raíces reservantes; peso de raíz reservante/ planta; contenido de materia seca y rendimiento (t.ha -1 . Todos los cultivares evaluados superaron el rendimiento de la variedad Criollo (6 t.ha -1 y el promedio nacional (5 a 7,8 t.ha -1 , con rendimientos entre 12 y 48 t.ha -1 . Algunos de los cultivares son promisorios para su cultivo en Costa Rica, no solo con base en su rendimiento, sino debido a su color de pulpa, amarilla o anaranjada, asociado a un mayor contenido de carotenos.

  18. in vitro production of virus free sweet potato [ipomoea batatas (l

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    the critical factor for the growth and enzyme production by Trichoderma isolates. ... brewing and wine making, agriculture biomass refining, pulp and paper ... chiefly produced by microorganisms such as ... plant symbionts (Chet and Baker, 1981 and ..... better stimulating effect than cellulose with ... sustainable development.

  19. in vitro production of virus free sweet potato [ipomoea batatas (l

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    water quality and several other problems ... the lake have emerged, including irrigation, construction .... Effect of zooplankton density change on grazing .... Ecological Structures and Functions, pp. ... green algae suitable food for zooplankton?

  20. In vitro bioaccessibility of beta-carotene in orange fleshed sweet potato (Ipomoea batatas, Lam.).

    Science.gov (United States)

    Failla, Mark L; Thakkar, Sagar K; Kim, Jung Y

    2009-11-25

    Substitution of white with orange fleshed varieties of sweet potatoes (OFSP) was recently shown to alleviate vitamin A deficiency in children in Africa. However, the relationship between beta-carotene (BC) content of different cultivars of OFSP and its bioavailability is unknown. Here, we used the three phase (oral, gastric and small intestinal) in vitro digestion procedure to examine the bioaccessibility of BC from eight cultivars of boiled OFSP. All-trans BC (all-E-BC) was the only isomer of BC detected in raw roots for cultivars of OFSP with amounts ranging from 112 to 281 microg/g. Boiling OFSP decreased all-E-BC content by 11% with conversion to 13-cis BC (13-Z-BC). The efficiency of BC micellarization during simulated digestion of boiled OFSP was only 0.6-3%. Addition of soybean oil (2% vol/wt) to boiled OFSP prior to in vitro digestion more than doubled partitioning of all-E-BC in the micelle fraction for all cultivars. The relatively poor bioaccessibility of all-E-BC was not a limitation of the in vitro model as micellarization was proportional to amount of OFSP digested from 0.5 to 3.0 g and minimally altered by increasing bile salt content during small intestinal digestion. Moreover, micellarization of all-E-BC from boiled fresh OFSP and commercially processed OFSP was significantly less than from carrots processed identically. These results indicate the need for further efforts to elucidate the basis for relatively poor bioaccessibility of BC from OFSP.

  1. in vitro production of virus free sweet potato [ipomoea batatas (l

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    This work revealed that helminth parasites are widespread in fish from Koka reservoir and. C. gariepinus was ... occupying a variety of marine and freshwater habitat (DACA, 2006). Fish ..... parasite fauna diversity could be affected by seasonal ...

  2. ALTERNATIVAS DE SIEMBRA DEL CAMOTE (Ipomoea batatas L. PARA EL CANTON JARAMIIJO, PROVINCIA DE MANABI

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    Nelson Enrique Motato Alarcón

    2016-06-01

    Full Text Available El trabajo se realizó entre agosto/2014 y enero /2015, en el cantón Jaramijó, provincia de Manabí, tuvo el propósito de generar información tecnológica relacionada con variedades (2, distancias entre plantas 3 y longitudes de guía (3, en el cultivo de camote; complementariamente se hizo un análisis económico de los 18 tratamientos, distribuidos en tres repeticiones, en un diseño de Bloques Completos al Azar bajo un Arreglo Factorial 2x3x3. El análisis estadístico de los datos: números de guía por planta, producción de follaje y número de camotes y rendimiento de raíces frescas por hectárea que permitieron determinar que la variedad Toquecita fue más productiva que Guayaco Morado, la mejor combinación fue la variedad Toquecita sembrada a 0,50 m o 0,30 m de distancia entre plantas y con guías de 0,50 m de tamaño. Económicamente, la alternativa con fines de comercialización externa y/o producción industrial de alimentos recomendada es la de utilizar Toquecita sembrada a 0,40 m o 0,50 m entre plantas y con guías de 0,30 m o 0,50 m de longitud; otra opción que considera la preferencia de los consumidores en el mercado nacional, es la de emplear Guayaco Morado, sembrado a 0,50 m entre plantas y con guías de 0,40 m o 0,50 m de longitud.

  3. Scanning of transposable elements and analyzing expression of transposase genes of sweet potato [Ipomoea batatas].

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    Lang Yan

    Full Text Available BACKGROUND: Transposable elements (TEs are the most abundant genomic components in eukaryotes and affect the genome by their replications and movements to generate genetic plasticity. Sweet potato performs asexual reproduction generally and the TEs may be an important genetic factor for genome reorganization. Complete identification of TEs is essential for the study of genome evolution. However, the TEs of sweet potato are still poorly understood because of its complex hexaploid genome and difficulty in genome sequencing. The recent availability of the sweet potato transcriptome databases provides an opportunity for discovering and characterizing the expressed TEs. METHODOLOGY/PRINCIPAL FINDINGS: We first established the integrated-transcriptome database by de novo assembling four published sweet potato transcriptome databases from three cultivars in China. Using sequence-similarity search and analysis, a total of 1,405 TEs including 883 retrotransposons and 522 DNA transposons were predicted and categorized. Depending on mapping sets of RNA-Seq raw short reads to the predicted TEs, we compared the quantities, classifications and expression activities of TEs inter- and intra-cultivars. Moreover, the differential expressions of TEs in seven tissues of Xushu 18 cultivar were analyzed by using Illumina digital gene expression (DGE tag profiling. It was found that 417 TEs were expressed in one or more tissues and 107 in all seven tissues. Furthermore, the copy number of 11 transposase genes was determined to be 1-3 copies in the genome of sweet potato by Real-time PCR-based absolute quantification. CONCLUSIONS/SIGNIFICANCE: Our result provides a new method for TE searching on species with transcriptome sequences while lacking genome information. The searching, identification and expression analysis of TEs will provide useful TE information in sweet potato, which are valuable for the further studies of TE-mediated gene mutation and optimization in asexual reproduction. It contributes to elucidating the roles of TEs in genome evolution.

  4. Postharvest Quality and Physiological Behavior of Sweet Potato (Ipomoea batatas Lam.) Leaf Stalks Under Three Temperatures

    Institute of Scientific and Technical Information of China (English)

    PENG Yan; XU Yong-quan; DUAN Dao-fu; MAO Lin-chun

    2009-01-01

    Sweet potato (lpomoea batatas Lam.) leaf stalks were cut into 20 cm length, and stored at 20, 6, and 2℃. The respiration rate, ethylene production, ascorbic acid, free amino acid, total chlorophyll content, freshness, and shelf life were determined during storage to investigate the effect of storage temperatures on the quality and physiological responses in sweet potato leaf stalks. Wound responses were observed as high respiration rate and ethylene production immediately after cutting. Sweet potato leaf stalks were found to be sensitive to chilling injury manifested as browning and water-soaking on the surface at 2℃. In contrast, sweet potato leaf stalks were susceptible to senescence, exhibited by etiolating and yellowing, at 20℃. Loss in weight and chlorophyll was minimized under low temperatures. High temperatures also caused the accumulation of amino acids with a significant loss of ascorbic acid and chlorophyll. Sweet potato leaf stalks had a storage life of 16 days at 6℃, 8 days at 2℃, and 6 days at 20℃, respectively.

  5. Assessment of the perinatal effects of maternal ingestion of Ipomoea carnea in rats

    Science.gov (United States)

    It is believed that I. carnea toxicosis induces abnormal embryogenesis in livestock. Studies with rats treated with I. carnea aqueous fraction (AF) during gestation, revealed litters with decreased body weight, but the characteristic vacuolar lesions promoted by swainsonine, its main toxic principle...

  6. Sweet potato (Ipomoea batatas) attenuates diet-induced aortic stiffening independent of changes in body composition.

    Science.gov (United States)

    Garner, Tyler; Ouyang, An; Berrones, Adam J; Campbell, Marilyn S; Du, Bing; Fleenor, Bradley S

    2017-08-01

    We hypothesized a sweet potato intervention would prevent high-fat (HF) diet-induced aortic stiffness, which would be associated with decreased arterial oxidative stress and increased mitochondrial uncoupling. Young (8-week old) C57BL/6J mice were randomly divided into 4 groups: low fat (LF; 10% fat), HF (60% fat), low-fat sweet potato (LFSP; 10% fat containing 260.3 μg/kcal sweet potato), or high-fat sweet potato diet (HFSP; 60% fat containing 260.3 μg/kcal sweet potato) for 16 weeks. Compared with LF and LFSP, HF- and HFSP-fed mice had increased body mass and percent fat mass with lower percent lean mass (all, P 0.05). Arterial stiffness, assessed by aortic pulse wave velocity and ex vivo mechanical testing of the elastin region elastic modulus (EEM) was greater in HF compared with LF and HFSP animals (all, P mitochondrial uncoupler, for 72 h reduced the EEM of HF arteries compared with nontreated HF segments (P mitochondrial uncoupling.

  7. Physiological responses of sweet potato (Ipomoea batatas L. plants due to different copper concentrations

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    Cristina Copstein Cuchiara

    2015-12-01

    Full Text Available At low concentrations, Cu is considered as an essential micronutrient for plants and as a constituent and activator of several enzymes. However, when in excess, Cu can negatively affect plant growth and metabolism. Therefore, the aim of this study was to evaluate physiological responses of sweet potato plants at different Cu concentrations by measuring morphological parameters, antioxidant metabolism, stomatal characteristics, and mineral profile. For this purpose, sweet potato plants were grown hydroponically in complete nutrient solution for six days. Then, the plants were transferred to solutions containing different Cu concentrations, 0.041 (control, 0.082, and 0.164 mM, and maintained for nine days. The main effect of increased Cu concentration was observed in the roots. The sweet potato plants grown in 0.082 mM Cu solution showed increased activity of antioxidant enzymes and no changes in growth parameters. However, at a concentration of 0.164 mM, Cu was transported from the roots to the shoots. This concentration altered morpho-anatomical characteristics and activated the antioxidant system because of the stress generated by excess Cu. On the basis of the results, it can be concluded that the sweet potato plants were able to tolerate Cu toxicity until 0.082 mM.

  8. A Comparative Study of Bioethanol Production from Aquatic Weeds

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    Kodichetty Ramaiah Sunil

    2015-09-01

    Full Text Available A greatest challenge for society in the 21st century is to meet energy demand, where biomass is subjected for pre-treatment and converted into biofuel (alcohol. Aquatic weeds are potential bio resources which are easily available for biofuel production. Aquatic weeds like Alternanthera sessilis, Typha latifolia, Eichhornia crassipes, Baccopa monnieri, Ipomoea aquatica and Pistia stratiotes are estimated for carbohydrates content. Highest content of reducing sugar was observed in Alternanthera sessilis (296.8µg/ml, total sugar in Ipomoea aquatic (880.00mg/ml, starch in Alternanthera sessilis (57.13mg/ml, cellulose in Pistia stratiotes and Typha latifolia (280.00mg/ml, hemicellulose in Typha latifolia (26.85mg/ml; high cellulosic aquatic weeds were subjected to pre-treatment methods like physical, chemical and enzymatic method. Meanwhile different yeast strains from the fruits of Manilkara zapota, Cucumis melo, Musa paradisiaca, Citrullus lanatus, Punica granatum and Ananas comosus were isolated yeast of Citrullus lanatus shows highest amount of alcohol production (307µg/ml, which is inoculated to pre-treated hydrolysate, where Alternanthera sessilis and Typha latifolia shows high amount of alcohol in physical method (160.5 and 115.4µg/ml. In chemical method in acid hydrolysis it shows 387.1 and 69.63µg/ml and in alkali hydrolysis 62 and 170µg/ml, so these two weeds were taken for enzymatic method for alcohol production, on seventh day Alternanthera sessilis shows highest alcohol production (113.33µg/ml, hence among six weeds Alternanthera sessilis and the yeast of Citrullus lanatus produces more amount of alcohol than others and it also shows that enzymatic method of pre-treatment is best in hydrolysis of biomass than physical and chemical method. The study revealed the possibility of producing alcohol from locally available fruits using simple, cheap and adaptable technology with biochemically characterized yeast strains.

  9. Fisioterapia aquatica: utilização do métado Bad Ragaz e do Water Pilates e em espondilite anquilosante: um estudo de caso

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    Bruno Gil Aldenucci

    2011-07-01

    Full Text Available A hidroterapia é um recurso fisioterapêutico que é muito utilizado no tratamento de espondilite anquilosante. Existem diversos recursos e métodos aquáticos para desenvolver um tratamento para este tipo de patologia, e este estudo vem com o intuito de mostrar a eficácia de dois métodos. O presente estudo foi realizado com um portador de Espondilite Anquilosante, utilizando goniometria e escala de dor como parâmetros de evolução. Foram realizados um total de 17 atendimentos utilizando os métodos bad ragaz e water pilates, onde o paciente mostrou significativa melhora da dor, três pontos na escala de dor, e ganho de movimento segundo tabela apresentada com os resultados da goniometria.

  10. Structural characterization of aquatic humic substance extracted from Itapanhau and Ribeira de Iguape rivers; Caracterizacao estrutural das substancias humicas aquaticas extraidas dos Rios Itapanhau e Ribeira de Iguape

    Energy Technology Data Exchange (ETDEWEB)

    Goveia, Danielle; Rocha, Julio Cesar [UNESP, Araraquara, SP (Brazil). Inst. de Quimica; Oliveira, Luciana Camargo de [Universidade Federal de Sao Carlos (UFSCar), Sorocaba, SP (Brazil); Morais, Leandro Cardoso de; Campos, Valquiria de; Fraceto, Leonardo Fernandes; Rosa, Andre Henrique [UNESP, Sorocaba, SP (Brazil). Dept. de Engenharia Ambiental

    2011-07-01

    The knowledge of the structure characteristic of the Organic Matter is important for the understanding of the natural process. In this context aquatic humic substances (principal fraction) were isolated from water sample collected from the two distinct rivers, using procedure recommended for International Humic Substances Society and characterized by elemental analysis, electron paramagnetic resonance and nuclear magnetic resonance (1{sup 3C} NMR). The results were interpreted using principal component analysis (PCA) and the statistical analyses showed different in the structural characteristics of the aquatic humic substances studied. (author)

  11. Evaluation of the toxicity of fluids employed in the metallic tool industrial machining using aquatic ecotoxicology;Avaliacao da toxicidade de fluidos de usinagem atraves da ecotoxicologia aquatica

    Energy Technology Data Exchange (ETDEWEB)

    Coelho, Ricardo dos Santos

    2006-07-01

    Eco toxicological analyses have being used to monitor environmental samples, industrial effluents and complex substances. With the objective to analyze the toxicity of cutting fluids used in the machinery industry, acute toxicity test with species of three different trophic levels: Vibrio fischeri, Daphnia similis, Daphnia laevis e Danio rerio, were performing. The samples of fluids were analyzed by COD, phenol, pH, color, density and surfactants. The physical and chemical parameters are the according with the brazilian law, CONAMA 357 (D.O.U. 2005). The results of the toxicity tests showed that the cutting fluids have high toxicity to the organisms used in this study and the gamma radiation treatment was not efficient to decrease the matrix. The biodegradation in soil demonstrated be effective to the cutting fluids and the indigenous bacteria were identified and isolated to possible treatment of soils contaminated with these kinds of substances. The monitoring and management of residues of cutting fluids are necessary to preservation of aquatic live, in consequence of their high toxicity. (author)

  12. Heavy metals in water, sediments and wetland plants in an aquatic ecosystem of tropical industrial region, India.

    Science.gov (United States)

    Rai, Prabhat Kumar

    2009-11-01

    Concentrations of heavy metals (Cu, Cr, Fe, Pb, Zn, Hg, Ni, and Cd) and macronutrients (Mn) were measured in industrial effluents, water, bottom sediments, and wetland plants from a reservoir, Govind Ballabh (G.B.) Pant Sagar, in Singrauli Industrial region, India. The discharge point of a thermal power plant, a coal mine, and chlor-alkali effluent into the G.B. Pant Sagar were selected as sampling sites with one reference site in order to compare the findings. The concentrations of heavy metals in filtered water, sieved sediment samples (0.4-63 microm), and wetland plants were determined with particle-induced X-ray emission. The collected plants were Aponogeton natans, L. Engl. & Krause, Cyperus rotundus, L., Hydrilla verticillata, (L.f.) Royle, Ipomoea aquatica, Forssk., Marsilea quadrifolia, L., Potamogeton pectinatus, L., Eichhornia crassipes, (Mart.) Solms Monogr., Lemna minor, L., Spirodela polyrhiza (L.) Schleid. Linnaea, Azolla pinnata, R.Br., Vallisneria spiralis, L., and Polygonum amphibium, L. In general, metal concentration showed a significant positive correlation between industrial effluent, lake water, and lake sediment (p macrophytes for pollution monitoring.

  13. Selection and application of endophytic bacterium Achromobacter xylosoxidans strain F3B for improving phytoremediation of phenolic pollutants.

    Science.gov (United States)

    Ho, Ying-Ning; Mathew, Dony Chacko; Hsiao, Shu-Chuan; Shih, Chun-Hao; Chien, Mei-Fang; Chiang, Hsing-Mei; Huang, Chieh-Chen

    2012-06-15

    While phytoremediation has been considered as an in situ bioprocess to remediate environmental contaminants, the application of functional endophytic bacteria within plants remains a potential strategy that could enhance the plants' efficiency in phytoremediation. In this study, 219 endophytes were isolated from plants that are predominantly located in a constructed wetland, including reed (Phragmites australis) and water spinach (Ipomoea aquatica). Twenty-five strains of the isolated endophytes utilize aromatic compounds as sole carbon source; Achromobacter xylosoxidans strain F3B was chosen for the in planta studies using the model plant Arabidopsis thaliana. Phylogenetic analysis indicated that those endophytic isolates of A. xylosoxidans formed a cluster within its species, and a specific real-time PCR detection method was developed for confirming the stability of the isolates in plants. In the presence of either catechol or phenol, inoculation of A. thaliana with F3B could extend into the root lengths and fresh weights to promote pollutants removal rates. These results demonstrate the potential of the endophytic F3B strain for helping plants to tolerate stress from aromatic compounds and to improve phytoremediation of phenolic pollutants.

  14. Nitrate removal and denitrification affected by soil characteristics in nitrate treatment wetlands.

    Science.gov (United States)

    Lin, Ying-Feng; Jing, Shuh-Ren; Lee, Der-Yuan; Chang, Yih-Feng; Shih, Kai-Chung

    2007-03-01

    Several small-scale surface flow constructed wetlands unplanted and planted (monoculture) with various macrophytes (Phragmites australis, Typha orientalis, Pennisetum purpureum, Ipomoea aquatica, and Pistia stratiotes) were established to continuously receive nitrate-contaminated groundwater. Soil characteristics and their effects on nitrate removal and soil denitrification were investigated. The results showed that planted wetland cells exhibited significantly higher (P wetland cell (1%, 0.11 microg N2O-N/g/h). However, the unplanted uncovered wetland cell showed a nitrate removal efficiency (55%) lower than but a soil denitrification rate (9.12 microg N2O-N/g/h) comparable to the planted cells. The nitrate removal rate correlated closely and positively with the soil denitrification rate for the planted cells, indicating that soil denitrification is an important process for removing nitrate in constructed wetlands. The results of nitrogen budget revealed that around 68.9-90.7% of the overall nitrogen removal could be attributed to the total denitrification. The soil denitrification rate was found to correlate significantly (P wetland soil, which accordingly were concluded as suitable indicators of soil denitrification rate and nitrate removal rate in nitrate treatment wetlands.

  15. PENGARUH LOGAM BERAT Cd, Pb TERHADAP PERUBAHAN WARNA BATANG DAN DAUN SAYURAN

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    Hening Widowati

    2011-04-01

    Full Text Available Cd, Pb heavy metal absorption in aquatic vegetables: Genjer (Limnocharis flava, Kangkung air (Ipomoea aquatica Forsk dan Selada air (Nasturtium officinale R. Br, studied to see the relationship with green figure scale. The research objective was to identify the accumulated Cd, Pb in the consumed organ vegetable, and their impacts to the levels of green figure scale. The research was undertaken by planting three kinds of the aquatic vegetables to the contaminated pure Cd,pure Pb media, mixture of Cd and Pb with factorial randomized block design. Data were analyzed by factorial One-Way Anova and further test of Duncan and HSD to see the difference of Cd, Pb and green figure scale, regression and correlation to know the contribution of Cd, Pb in influencing the green figure scale. Variety of vegetables, media, organ, and the interaction influences the accumulation of Cd, Pb and level of green figure scale. There is a relationship between Cd, Pb metal accumulation to the level of green figure scale of the aquatic vegetables, with negative correlation that Cd, Pb decreased the vegetable green figure scale.

  16. Screening of plants acting against Heterometrus laoticus scorpion venom activity on fibroblast cell lysis.

    Science.gov (United States)

    Uawonggul, Nunthawun; Chaveerach, Arunrat; Thammasirirak, Sompong; Arkaravichien, Tarinee; Chuachan, Chattong; Daduang, Sakda

    2006-01-16

    The aqueous extracts of 64 plant species, listed as animal- or insect-bite antidotes in old Thai drug recipes were screened for their activity against fibroblast cell lysis after Heterometrus laoticus scorpion venom treatment. The venom was preincubated with plant extract for 30 min and furthered treated to confluent fibroblast cells for 30 min. More than 40% efficiency (test/control) was obtained from cell treatment with venom preincubated with extracts of Andrographis paniculata Nees (Acanthaceae), Barringtonia acutangula (L.) Gaertn. (Lecythidaceae), Calamus sp. (Palmae), Clinacanthus nutans Lindau (Acanthaceae), Euphorbia neriifolia L. (Euphorbiaceae), Ipomoea aquatica Forssk (Convolvulaceae), Mesua ferrea L. (Guttiferae), Passiflora laurifolia L. (Passifloraceae), Plectranthus amboinicus (Lour.) Spreng. (Labiatae), Ricinus communis L. (Euphorbiaceae), Rumex sp. (Polygonaceae) and Sapindus rarak DC. (Sapindaceae), indicating that they had a tendency to be scorpion venom antidotes. However, only Andrographis paniculata and Barringtonia acutangula extracts provided around 50% viable cells from extract treatments without venom preincubation. These two plant extracts are expected to be scorpion venom antidotes with low cytotoxicity.

  17. Assessment of veterinary drugs in plants using pharmacokinetic approaches: The absorption, distribution and elimination of tetracycline and sulfamethoxazole in ephemeral vegetables.

    Science.gov (United States)

    Chen, Hui-Ru; Rairat, Tirawat; Loh, Shih-Hurng; Wu, Yu-Chieh; Vickroy, Thomas W; Chou, Chi-Chung

    2017-01-01

    The present study was carried out to demonstrate novel use of pharmacokinetic approaches to characterize drug behaviors/movements in the vegetables with implications to food safety. The absorption, distribution, metabolism and most importantly, the elimination of tetracycline (TC) and sulfamethoxazole (SMX) in edible plants Brassica rapa chinensis and Ipomoea aquatica grown hydroponically were demonstrated and studied using non-compartmental pharmacokinetic analysis. The results revealed drug-dependent and vegetable-dependent pharmacokinetic differences and indicated that ephemeral vegetables could have high capacity accumulating antibiotics (up to 160 μg g-1 for TC and 38 μg g-1 for SMX) within hours. TC concentration in the root (Cmax) could reach 11 times higher than that in the cultivation fluid and 3-28 times higher than the petioles/stems. Based on the volume of distribution (Vss), SMX was 3-6 times more extensively distributed than TC. Both antibiotics showed evident, albeit slow elimination phase with elimination half-lives ranging from 22 to 88 hours. For the first time drug elimination through the roots of a plant was demonstrated, and by viewing the root as a central compartment and continuous infusion without a loading dose as drug administration mode, it is possible to pharmacokinetically monitor the movement of antibiotics and their fate in the vegetables with more detailed information not previously available. Phyto-pharmacokinetic could be a new area worth developing new models for the assessment of veterinary drugs in edible plants.

  18. Transfer of oxytetracycline from swine manure to three different aquatic plants: implications for human exposure.

    Science.gov (United States)

    Boonsaner, Maliwan; Hawker, Darryl W

    2015-03-01

    Little is known regarding the potential for pharmaceuticals including antibiotics to be accumulated in edible aquatic plants and enter the human food chain. This work investigates the transfer of a widely used veterinary antibiotic, oxytetracycline (OTC), from swine manure to aquatic plants by firstly characterizing desorption from swine manure to water and fitting data to both nonlinear and linear isotherms. Bioconcentration of OTC from water was then quantified with aquatic plants of contrasting morphology and growth habit viz. watermeal (Wolffia globosa Hartog and Plas), cabomba (Cabomba caroliniana A. Gray) and water spinach (Ipomoea aquatica Forsk.). Watermeal and water spinach are widely consumed in Southeast Asia. The OTC desorption and bioconcentration data were used to provide the first quantitative estimates of human exposure to OTC from a manure-water-aquatic plant route. Results show that under certain conditions (plants growing for 15d in undiluted swine manure effluent (2% w/v solids) and an initial OTC swine manure concentration of 43mgkg(-1) (dry weight)), this pathway could provide a significant fraction (>48%) of the acceptable daily intake (ADI) for OTC. While effluent dilution, lower OTC manure concentrations and not all plant material consumed being contaminated would be expected to diminish the proportion of the ADI accumulated, uptake from aquatic plants should not be ignored when determining human exposure to antibiotics such as OTC.

  19. 3种水培植物根系分泌的有机酸对氮循环菌的影响%Effect of organic acids exuded from hydroponic plants roots on nitrogen cycling bacteria

    Institute of Scientific and Technical Information of China (English)

    朱静平; 程凯

    2011-01-01

    通过收集水培吊兰、空心菜和水芹的根系分泌物,采用液相色谱分析了其中有机酸的种类和含量;并研究了根系分泌物中的有机酸对氮循环菌的影响。结果表明3,种植物根系分泌物中的有机酸对氨化细菌和反硝化细菌的生长具有促进作用,对亚硝化细菌和硝化细菌的生长具有抑制作用。%The variety and content of organic acids exuded from hydroponic Chlorophytum comosum roots,hydroponic Ipomoea aquatica roots and hydroponic Oenanthe javanica roots by liquid chromatography were studied,and the effects of organic acid of root exudates on nitrogen cycling bacteria were analysed.The results showed that organic acid of the three hydroponic plants root exudates has promotion effect on ammonifying bacteria and denitrifying bacteria,but has inhibition effect on nitrobacteria and nitrosobacteria.

  20. Growth characteristics of mung beans and water convolvuluses exposed to 425-MHz electromagnetic fields.

    Science.gov (United States)

    Jinapang, Peeraya; Prakob, Panida; Wongwattananard, Pongtorn; Islam, Naz E; Kirawanich, Phumin

    2010-10-01

    Effects of high-frequency, continuous wave (CW) electromagnetic fields on mung beans (Vigna radiata L.) and water convolvuluses (Ipomoea aquatica Forssk.) were studied at different growth stages (pre-sown seed and early seedling). Specifically, the effects of the electromagnetic source's power and duration (defined as power-duration level) on the growth of the two species were studied. Mung beans and water convolvuluses were exposed to electromagnetic fields inside a specially designed chamber for optimum field absorption, and the responses of the seeds to a constant frequency at various power levels and durations of exposure were monitored. The frequency used in the experiments was 425 MHz, the field strengths were 1 mW, 100 mW, and 10 W, and the exposure durations were 1, 2, and 4 h. Results show that germination enhancement is optimum for the mung beans at 100 mW/1 h power-duration level, while for water convolvuluses the optimum germination power-duration level was 1 mW/2 h. When both seed types were exposed at the early sprouting phase with their respective optimum power-duration levels for optimum seed growth, water convolvuluses showed growth enhancement while mung bean sprouts showed no effects. Water content analysis of the seeds suggests thermal effects only at higher field strength.

  1. Development of constructed wetland using hydroponic biofilter method for purification of hyper-eutrophic lake water; Fueiyoka kosui no joka no tameno suiko seibutsu rokaho wo mochiita jinko shicchi no kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    Aizaki, M. [Shimane Univ., Matsue (Japan)] Nakasato, H. [Top Ecology Co. Ltd., Tokyo (Japan)

    1997-09-10

    Applying the hydroponic biofilter method as a direct purification method for a hyper-eutrophic lake water, an experiment was carried out at the Tsuchiura Port on Lake Kasumigaura to obtain data for constructing a hydrophilic artificial wetland. Purification of hyper-eutrophic lake water containing a large amount of water blooms in summer was attempted applying the hydroponic biofilter method for which hydrophyte is used. As a result, it was clarified, by applying the hydroponic biofilter method, that capturing effect of suspended substances can be achieved in the rooting zone, captured suspended substances are decomposed at high rate, and the revolved nutrient salt can be absorbed and assimilated by the use of plants having high growth rates. Ipomoea aquatica had the highest removal activity, followed by nasturtium officinal, menthe spicata, and oenanthe javanica. As a result, it became clear that a constructed wetland made with the hydroponic biofilter method can be applied as a direct purifying method for hyper-eutrophic lake water by selecting appropriate plants in accordance with season. 18 refs., 1 fig., 4 tabs.

  2. Storage Quality Comparisons of Coriander and Water Spinach Between Red Light and Darkness at Room Temperature%光线照射下芫荽和蕹菜常温贮藏效果比较

    Institute of Scientific and Technical Information of China (English)

    顾振新; 陆兆新; 韩永斌; 郁志芳; 魏秀娟

    2003-01-01

    比较了芫荽(Coriandrum sativum L.)和蕹菜(Ipomoea aquatica Forsk)在20±1℃下光照处理的贮藏效果.在4d贮藏期内,用光合量子通量密度(PPED)为5μmol/m2·s的红光每天连续照射24h.结果表明,与黑暗条件下芫荽和蕹菜贮藏效果相比,光照有利于累积还原糖和减少叶绿素和VC损失,并且光照条件下蕹菜的贮藏效果好于芫荽;贮藏结束时,光照处理的芫荽和蕹菜中叶绿素含量分别比同期黑暗处理的高出76.82%和93.36%,还原糖含量分别高出32.69%和20.57%,VC保存率分别高出2.31和2.38倍;芫荽根部和蕹菜嫩茎切口吸收水分而使其保持鲜嫩状态,两者含水量变化与光照无关.

  3. Selecting iodine-enriched vegetables and the residual effect of iodate application to soil.

    Science.gov (United States)

    Dai, Jiu-Lan; Zhu, Yong-Guan; Zhang, Min; Huang, Yi-Zhong

    2004-12-01

    A greenhouse pot experiment was conducted to select vegetables for iodine uptake. The residual effect of iodate fertilization on the growth of and iodine uptake by spinach plants were also investigated. Six vegetables, including leafy vegetables (pakchoi [Brassica chinensis L.], spinach [Spinacia oleracea L.]), tuber vegetables (onion [Allium cepa L.]), shoot vegetables (water spinach [Ipomoea aquatica Forsk.], celery [Apium graveolens L.]), and root vegetables (carrot [Daucus carota var. sativa DC.]) were examined. Results showed that the concentrations of iodate in soil had significant effect on the biomass of edible parts of pakchoi and spinach (pIodine concentrations in edible parts of vegetables and the transfer factors (TFedible parts) of soil-to-edible parts of vegetables significantly increased with increasing iodine concentrations in soil (piodine concentrations in edible parts and TFedible parts of spinach were much higher than those of other vegetables at any treatment. Both transfer coefficients for edible parts (TCedible parts) and for aerial parts (TCaerial parts) of vegetables changed differently with increasing iodine concentrations in the soil, and TCedible parts and TCaerial parts of spinach were higher than those of other vegetables. Therefore, spinach was considered as an efficient vegetable for iodine biofortification. Further experiment showed that there is considerable residual effect of soil fertilization with iodate.

  4. Caracterização de variedades de batata doce (Ipomoea batatas L. através de descritores morfológicos e isoenzimáticos Morphological and isoenzymatic characterization of sweet potato (Ipomoea batatas L.

    Directory of Open Access Journals (Sweden)

    Eliane Augustin

    2000-03-01

    Full Text Available Ocorre grande variabilidade no germoplasma de batata-doce disponível no Rio Grande do Sul, mas nem todas populações podem ser caracterizadas através de aspectos morfológicos, havendo necessidade de recorrer a outros métodos, como análise de isoenzimas, para a sua diferenciação. A avaliação de características morfológicas e isoenzimáticas de dez acessos do Banco de Germoplasma de Hortaliças da Embrapa Clima Temperado foi o objetivo deste trabalho. Quatro descritores morfológicos foram utilizados, permitindo distinguir seis variedades. Análises de isoenzimas de peroxidase, aspartato transaminase e esterase foram efetuadas, utilizando-se eletroforese horizontal em gel de poliacrilamida. Foi observada similaridade máxima (1,00 estimada através do coeficiente de Jaccard, com base nesses marcadores, apenas entre as variedades Americana e Morada INTA. A análise de agrupamento permitiu a distinção de dois grupos e quatro subgrupos, utilizando UPGMA (método da média aritmética não ponderada. Os resultados obtidos permitiram a diferenciação dos dez acessos estudados e indicaram que a análise de isoenzimas associada a avaliações morfológicas é de grande valia na caracterização de germoplasma de batata-doce disponível no Rio Grande do Sul.Despite the variability observed in sweet potato germplasm found in the Rio Grande do Sul State, many populations can not be morphologically characterized without the use of additional methods, such as isoenzyme analysis. The aim of this work was to evaluate the morphological and isoenzymatic characteristics of ten accesses of the genetic resources program of Embrapa Clima Temperado. Four morphological descriptors were used, which allowed to distinguish six varieties. Polyacrylamyde electrophoresis was used to analyze peroxidase, aspartate transaminase and esterase isoenzymes. Maximum similarity (1.00, estimated through Jaccard coefficient, was observed only in comparisons between Americana and Morada INTA. Varieties were classified into two groups and four subgroups, using UPGMA (unweighted pair-group method with arithmetic averages. All accesses could be differentiated. The results obtained indicated that isoenzyme analysis, associated to morphological evaluations, can be succesfully used to characterize sweet potato germplasm available in the Rio Grande do Sul.

  5. PENGEMBANGAN PRODUK MI KERING DARI TEPUNG UBI JALAR (Ipomoea batatas DAN PENENTUAN UMUR SIMPANNYA DENGAN METODE ISOTERM SORPSI [Development of Dried Noodle Made of Sweet Potato (Ipomoea batatas Flour and Prediction of Its Shelf Life Using Sorption Isotherm Method

    Directory of Open Access Journals (Sweden)

    Sugiyono1*

    2011-12-01

    Full Text Available The objective of this research was to develop dried noodle product made of sweet potato flour and to predict its shelf life using sorption isotherm method. The product was produced through gelatinization of dough, followed by sheeting, steaming, and drying. The best quality product was produced through gelatinization for 20 minutes followed by steaming for 10 minutes. The best quality product had 5.86% moisture, 3.10% ash, 0.23% fat, 2.66% protein, and 88.15% carbohydrate. The product had 54.43 degree of whiteness, 28.75 gf of elasticity, 3.5 minutes of rehydration time, 53.23% of water absorption capacity, and 14.85% of cooking loss. The sorption isotherm curve of the product was best described by Henderson model. The shelf lives of the packed product in low density polyethylene, medium density polyethylene, high density polyethylene, and polypropylene at 80% RH were calculated to be 55, 92, 277 and 150 days respectively.

  6. Cloning and Sequence Analysis of 18SrRNA Gene in Ipomoea batatas and Ipomoea setosa%甘薯和巴西牵牛18S rRNA基因的克隆和序列分析

    Institute of Scientific and Technical Information of China (English)

    王振东; 王晓华; 乔奇; 张德胜; 秦艳红; 田雨婷; 张振臣

    2011-01-01

    [Objective]to supply sequence information of internal control gene for analyzing gene expression of I.batatas and viruses infecting L batatas.[Method]Sequences of 18S rRNA gene were cloned using PCR method from genomic DNA of I.batatas cultivar 'Shangshu19', 'Beijing553' and I.setosa, respectively.[Result]The sequencing of the DNA fragments all generated a total of 1630 bp nucleotide sequence.The obtained 18S rRNA gene sequences of I.batatas and I.setosa shared more than 98% identity with I.hederaceaand Nicotiana tabacum among dicotyledons, and shared high identity with Lilium superbum among monocotyledons.[Conclusion]Partial sequences of 18S rRNA gene were cloned from genomic DNA of I.batatas cultivar 'Shangshu19','Beijing553' and I.setosa, which provided sequence information not only for analyzing gene expression of I.batatas and viruses infecting I.batatas using 18S rRNA gene as internal control, but for molecular systematic research of I.batatas and I.setosa.%[研究目的]为甘薯和侵染甘薯病毒的基因表达研究提供内参基因序列信息.[方法]分别以'商薯19'、'北京553'2个甘薯品种和巴西牵牛的基因组DNA为模板,利用PCR方法克隆甘薯和巴西牵牛18S rRNA基因序列.[结果]测序结果表明,获得的供试2甘薯品种和巴西牵牛的18S rRNA基因序列长度均为1630 bp;序列比对结果表明,甘薯和巴西牵牛与裂叶牵牛、烟草等双子叶植物的18S rRNA基因相应序列的一致性均达98%以上,与单子叶植物Lilium superbum的18S rRNA基因序列也有较高的一致性.[结论]从2甘薯品种和巴西牵牛的基因组中克隆出了18S rRNA基因部分序列,研究结果不仅为利用18S rRNA基因作为内参基因分析甘薯和侵染甘薯病毒基因的表达研究提供了序列依据,而且可为甘薯和巴西牵牛的分子系统学研究提供序列参考.

  7. Escuela de campo de agricultores para el fitomejoramiento participativo del boniato (Ipomoea batatas (L. Lam.. Farmer´s Field School for the sweet potato (Ipomoea batatas (L. Lam. participatory breeding.

    Directory of Open Access Journals (Sweden)

    Mario Díaz López

    2013-08-01

    Full Text Available Resumen.Se presenta una Escuela de Campo para capacitar a los agricultores de sub urbanos en el Fitomejoramiento Participativo del boniato. En su concepción se emplearon métodos del nivel teórico (análisis – síntesis, inducción - deducción, histórico y lógico, así como del nivel empírico (entrevistas, entrevistas a grupos focales, análisis de documentos, observación, encuestas que permitieron integrar el Fitomejoramiento Participativo con la capacitación de los agricultores. Se discuten los aspectos metodológicos relacionados con su aplicación. Abstract. A Farmer´s Field School is presented to train farmers in the sub urban Participatory Plant Breeding of sweet potato. And the empirical level (interviews, focus group interviews, document analysis, observation, surveys that allowed integrating Participatory Plant Breeding in concept with theoretical methods (analysis,- synthesis, induction -deduction, historical and logical analysis were used training of farmers. methodological issues related to its implementation are discussed.

  8. Advances in Storage Techniques of Sweetpotato (Ipomoea batatas (L.) Lam.) Germplasm In Vitro%甘薯(Ipomoea batatas(L.) Lam.)试管苗种质保存技术研究进展

    Institute of Scientific and Technical Information of China (English)

    张海燕; 张立明; 王庆美; 李爱贤; 孙立荣

    2005-01-01

    甘薯种质资源的大田种植保存面临着严重的病毒感染以及病虫害、自然灾害的影响.由茎尖分生组织培养成无病毒苗并进行试管保存,是当前既经济又实用的方法.

  9. Study on polysaccharide extracting technology from stem of sweet potato(Ipomoea batatas(L.)Lam.)%甘薯Ipomoea batatas(L.) Lam.茎蔓多糖提取技术研究

    Institute of Scientific and Technical Information of China (English)

    王宪昌; 呼晓姝; 王建中

    2005-01-01

    通过正交实验研究了甘薯茎蔓多糖水提过程中提取温度、时间和提取用水量对提取物得率的影响,选出了较为理想的提取条件,即加40倍水,在100℃下浸提25 min,得率4.82%.通过棕色环法、蒽酮-硫酸法、Fehling试剂法、双缩脲法证实提取物为多糖.

  10. EVALUATION OF TWO BRASSINOSTEROID ANALOGUES ON CALLUS FORMATION AND SHOOT INDUCTION IN SWEET POTATO (Ipomoea batatas (L. Lam.

    Directory of Open Access Journals (Sweden)

    O. González

    2003-01-01

    Full Text Available Se seleccionaron explantes del limbo foliar y se sembraron en el medio de cultivo de Murashige y Skoog con 2,4-D (0.1 mg.L-1 y 6-BAP (0.2 mg.L-1, combinados con los análogos de brasinoesteroides Biobras-6 (BB-6 o MH-5 a diferentes concentraciones (0.001, 0.01, 0.05 y 0.1 mg.L-1 y los análogos de brasinosteroides independientes o combinados con 2,4-D para inducir la formación de callos. Después de cuatro semanas se evaluó el porcentaje de callos formados. Para estudiar la influencia de los análogos seleccionados en la inducción de regenerantes, se utilizaron callos de cuatro semanas obtenidos en el medio control (2,4-D + 6-BAP y se transfirieron a medios que contenían BB-6 o MH-5 solamente o en combinación con AIA (0.05 mg.L-1 o con AIA (0.05 mg.L-1 y Kinetina (0.2 mg.L-1. Los callos se mantuvieron en cámara de incubación en condiciones de luz solar a la temperatura de 25 ± 2°C, humedad relativa de 80-90 % e intensidad luminosa de 72-90 ¿mol.m-2.S-1. Transcurridas cuatro semanas, se realizaron mediciones cuantitativas y cualitativas a los callos y los regenerantes procedentes de los callos obtenidos. Se lograron buenos resultados en la formación de callos morfogénicos, al emplear 2,4-D (0.1 mg.L-1 y 6-BAP (0.2 mg.L-1 como únicos reguladores del crecimiento en el medio de cultivo y al ser combinados con los análogos de brasinoesteroides BB-6 o MH-5 a la concentración de 0.01 mg.L-1. La inducción de regenerantes se vio favorecida, de manera general, con el empleo del análogo de brasinoesteroides combinado con AIA (0.05 mg.L-1 y Kinetina (0.2 mg.L-1, en comparación con los análogos independientes en el medio de cultivo.

  11. Toxic Ipomeamarone Accumulation in Healthy Parts of Sweetpotato (Ipomoea batatas L. Lam) Storage Roots upon Infection by Rhizopus stolonifer

    Science.gov (United States)

    2015-01-01

    Furanoterpenoid accumulation in response to microbial attack in rotting sweetpotatoes has long been linked to deaths and lung edema of cattle in the world. However, it is not known whether furanoterpenoid ipomeamarone accumulates in the healthy-looking parts of infected sweetpotato storage roots. This is critical for effective utilization as animal feed and assessment of the potential negative impact on human health. Therefore, we first identified the fungus from infected sweetpotatoes as a Rhizopus stolonifer strain and then used it to infect healthy sweetpotato storage roots for characterization of furanoterpenoid content. Ipomeamarone and its precursor, dehydroipomeamarone, were identified through spectroscopic analyses, and detected in all samples and controls at varying concentrations. Ipomeamarone concentration was at toxic levels in healthy-looking parts of some samples. Our study provides fundamental information on furanoterpenoids in relation to high levels reported that could subsequently affect cattle on consumption and high ipomeamarone levels in healthy-looking parts. PMID:25418792

  12. Comparative analysis of phytochemicals and nutrient availability in two contrasting cultivars of sweet potato (Ipomoea batatas L.).

    Science.gov (United States)

    Shekhar, Shubhendu; Mishra, Divya; Buragohain, Alak Kumar; Chakraborty, Subhra; Chakraborty, Niranjan

    2015-04-15

    Sweet potato ranks as the world's seventh most important food crop, and has major contribution to energy and phytochemical source of nutrition. To unravel the molecular basis for differential nutrient availability, and to exploit the natural genetic variation(s) of sweet potato, a series of physiochemical and proteomics experiment was conducted using two contrasting cultivars, an orange-fleshed sweet potato (OFSP) and a white-fleshed sweet potato (WFSP). Phytochemical screening revealed high percentage of carbohydrate, reducing sugar and phenolics in WFSP, whereas OFSP showed increased levels of total protein, flavonoids, anthocyanins, and carotenoids. The rate of starch and cellulose degradation was found to be less in OFSP during storage, indicating tight regulation of gene(s) responsible for starch-degradation. Comparative proteomics displayed a cultivar-dependent expression of proteins along with evolutionarily conserved proteins. These results suggest that cultivar-specific expression of proteins and/or their interacting partners might play a crucial role for nutrient acquisition in sweet potato.

  13. Improvement of sperm density in neem-oil induced infertile male albino rats by Ipomoea digitata Linn.

    Directory of Open Access Journals (Sweden)

    Ghanashyam Keshav Mahajan

    2015-06-01

    Full Text Available Aim: Investigation has been carried out to validate folkloric claim of the potential of ID based on reproductive health status in experimentally induced male albino rats. Materials and Methods: Emulsified neem oil (ENO fed albino rats were orally administered root powder of ID suspended in water for the doses of 250 and 500 mg/kg body weight for 40 days. Change in organ weight, sperm density and motility, serum hormonal levels and histomorphological changes were evaluated. Results: Significant increase in the sperm density and the sperm motility (P< 0.01 along with increase in the testis, and epididymes weight in neem-oil induced infertile rats treated with ID at both dose levels. This effect is vis- and agrave;-vis to serum hormonal levels. Presence of beta-sitosterol in the root of ID likely to enhance the process of spermatogenesis as it is evident from histomorphological studies. Conclusion: Results of the present investigation reveal that ID is a good candidate for the management of male infertility. [J Intercult Ethnopharmacol 2015; 4(2.000: 125-128

  14. INIVIT B-96, UN CLON DE BONIATO (Ipomoea batatas L.) DE MASA ANARANJADA "CON ALTO CONTENIDO DE B-CAROTENOS"

    OpenAIRE

    Rodríguez, J.E.; L. Arias

    2002-01-01

    Se presentan las principales características de un nuevo clon de boniato, obtenido a partir de un material colectado en Camagüey, mediante el empleo de métodos de mejoramiento convencional, tales como la selección de plantas élite y producción de semilla original a partir de tubérculos seleccionados de estas. El clon INIVIT B-96 mostró alto potencial de rendimiento, calidad en la producción de semilla agámica, alta velocidad de cobertura del área por el follaje así como altosvolúmenes de prod...

  15. 蕹菜的DAPI显带核型%DAPI Banded Karyotype of Ipomoea aquantica Forsk (I. reptans Poir)

    Institute of Scientific and Technical Information of China (English)

    刁英; 陈思; 黄雨蝶; 周明全; 胡中立

    2005-01-01

    利用一种新的DAPI显带技术分析了蕹菜染色体的DAPI带型及其异染色质的分布,建立了类似于G带的蕹菜DAPI带模式图,为蕹菜的细胞遗传学研究、育种和资源的开发利用提供帮助.

  16. Sweet potato [Ipomoea batatas (L.) Lam.] cultivated as tuber or leafy vegetable supplier as affected by elevated tropospheric ozone.

    Science.gov (United States)

    Keutgen, Norbert; Keutgen, Anna J; Janssens, Marc J J

    2008-08-13

    Sweet potato cultivars respond differently to elevated tropospheric ozone concentrations of ca. 130 mug m (-3), 8 h a day for 4 weeks, which affects their selection for cultivation. In the first cultivar presented here, an adequate leafy vegetable supplier, the ozone load resulted in a shift of biomass to maintain the canopy at the expense of tuber development. Starch content of leaves was reduced, indicating an impairment of quality, but carotenoid content remained stable. The second cultivar may be grown for tuber production. Although the ratio tuber/plant remained stable under ozone, tuber yield and its starch content were significantly reduced. The lower starch content indicated a worse quality for certain industrial processing, but it is desirable for chip production. Elevated tropospheric ozone concentrations also influenced free amino acids and macronutrient contents of tubers, but these modifications were of minor significance for tuber quality in the second cultivar.

  17. Characteristics of Fermentation Drink made from Juice of Winged Bean Sprouts (Psophocarpus tetragonolobus and Red Sweet Potato (Ipomoea batatas

    Directory of Open Access Journals (Sweden)

    Novelina Novelina

    2013-01-01

    Full Text Available Fermented milk is a product that was produced from fermentation of fresh milk using lactic acid bacteria, such as Lactobacillus bulgaricus and Streptococcus thermophilus. A variety of plant materials can also be used as raw material in making of  fermentation drinks, such as nuts and tubers. The purpose of this study was to investigate the characteristics of fermentation drink made from juice of winged bean sprout and red sweet potatoes. The treatment in this study is the level of mixing between juice of winged bean sprout with red sweet potatoes , consist of ;A (30:70, B (40:60, C (50:50, D (60:40 and E (70:30. Completely randomized design had been used with 5 treatments and 3 replications. The data colected was pH, viscosity, protein content, fat content, total solids, total acid, ash content, calcium content, total lactic acid bacteria, total plate count and sensory evaluation. Data processing is performed using statistical 8, followed by Tuckey test HSD All-Pair wise Comparisons Test at level of 5%. Mixing of winged bean sprouts with red sweet potato can increase the value of pH, viscosity, protein content, fat content, total solids, total acid, ash content, calcium content, and total lactic acid bacteria. The best product was the product of B (40% winged bean sprout and 60% red sweet potatoes already meets SNI 7552:2009, contain : 2.36% protein content, fat content of 1.24%, 18.77% total solids, pH was 4, 2, 0.54% total acid, 0.23% ash content, 0.173% calcium content, viscosity was 3.56 DPA,  total  lactic acid bacteria (CFU / ml was 1.9 x1010 , total plate count was 2.1 x1010 (CFU / ml.

  18. 甘薯微繁殖技术研究%Study on Micropropagation of Ipomoea batatas Lam.

    Institute of Scientific and Technical Information of China (English)

    刘进平; 吴繁花

    2004-01-01

    对用甘薯离体培养进行了研究,以带芽茎段培养(微型扦插)可实现增殖.最适启动培养基为1/2MS+白糖30 g/L+卡拉胶10 g/L+NAA 0.2 mg/L+BA 0.5 mg/L,最适增殖及生根培养基为1/2MS+白糖30 g/L+卡拉胶10 g/L+NAA 0.2 mg/L+BA 0.5 mg/L.

  19. Effect of processing techniques on color and active components amount of sweet potato (Ipomoea Batatas l) flakes

    Science.gov (United States)

    Rahmawati, Y.; Mahmudatussa'adah, A.; Yogha, S.

    2016-04-01

    Sweet potato processing is limited, such as flour, snacks, cystic, or chips. Flakes as pre-cooked meals are made through the stages of making pasta and drying. The purpose of this study was to optimize the production of sweet potato flakes at the stage of making pasta and drying. Making the pasta is done through techniques steamed or baked. Pasta drying using tools a drum dryer or cabinet dryer. As an indicator of optimization is the total of monomeric anthocyanins, β-carotene and color the resulting flakes. The results showed that the amount of anthocyanin monomeric flakes by using steam, and drum dryer (3.83 ± 0.03 mg CYE/g db), flakes by the technique of steam, and cabinet dryer (3.03 ± 0.02 mg CYE/g db), flakes with techniques bake, drum dryer (2.49 ± 0.05 CYE mg/g db), flakes with bake technique, cabinet dryer (1.98 ± 0.03 mg CYE/g db). The Color of purple sweet potato flakes produced through steamed techniques bright purple, while the color purple sweet potato flakes produced through techniques roast give a brownish purple color. The amount of β-carotene yellow flakes sweet potato with stages of cooking steamed, drum dryer (152±0.5 mg/Kg db), grilled drum dryer (136±0.4 mg/Kg db), flakes of yellow sweet potato with stages of roasted and cabinet dryer (140±0.8 mg/Kg db), and grilled stage with cabinet dryer (122±0.3 mg/Kg db). In conclusion sweet potato flakes production techniques through the stages of steam process, and used drum dryers have a number of anthocyanins or β-carotene bigger and brighter colors than the baked flakes techniques and used cabinet dryer.

  20. Field studies of sweet potatoes and cowpeas in response to elevated carbon dioxide. Progress report. [Ipomoea batatas; Vigna unguiculata

    Energy Technology Data Exchange (ETDEWEB)

    Biswas, P.K.

    1985-01-01

    The physiological and biochemical effects of enriched CO/sub 2/ on sweet potatoes and cowpeas were studied in open top chambers. The main task was to assemble equipment to generate test atmospheres of CO/sub 2/ in the field, using open top chambers as the basic exposure unit for studying the response of sweet potatoes and cowpeas to enriched CO/sub 2/. Plant responses to CO/sub 2/ in open top chambers have been demonstrated by several investigators in a variety of crops. This study focused on growth and development of both sweet potatoes and cowpeas at levels of CO/sub 2/ ranging from the ambient level of 354 ppM to 659 ppM. The effects of CO/sub 2/ on leaf and stem weights, stem length, leaf area, and stomatal number and conductance were studied on both species. Additional studies on sweet potatoes included the effects of CO/sub 2/ on the weight, chemical content and quality of tubers. Additional studies on cowpeas included the effects of CO/sub 2/ on the weight of seeds and rate of nitrogen fixation. 27 figs., 35 tabs. (ACR)

  1. Effect of NAA on cottage of Ipomoea L.%NAA对甘薯属植物扦插的影响

    Institute of Scientific and Technical Information of China (English)

    侯汲虹; 陈良钊; 朱宏波

    2011-01-01

    研究不同浓度NAA对5种甘薯属植物枝条扦插繁殖的影响.结果表明:五爪金龙(I.cairica)和紫心牵牛(I.obscura)很难扦插成活,另外3种植物的成活率由高到低依次为厚藤(I.pes-caprae)、海滩牵牛(Lstolonifera)、三裂叶薯(I.triloba);NAA对3种植物的成活率、根和芽的诱导具有不同程度的影响,其中三裂叶薯对NAA的敏感度最高.

  2. Modeling of mass transfer performance of hot-air drying of sweet potato (Ipomoea Batatas L.) slices

    National Research Council Canada - National Science Library

    Zhu Aishi; Jiang Feiyan

    2014-01-01

    ... velocity and thickness of sweet potato slice on the drying process. The experimental data of moisture ratio of sweet potato slices were used to fit the mathematical models, and the effective diffusion coefficients were calculated...

  3. Agrothechnical System for continuous production of sweet potatoes (Ipomoea batatas Lam in Trinidad´s City.

    Directory of Open Access Journals (Sweden)

    Juan Antonio Magdaleno Ortiz

    2012-04-01

    Full Text Available In prospective study different types of clones were identified recommended for the conditions edafoclimáticas of this municipality. That is why the objective of this work is to evaluate the productive results regarding to the potentialities of each established clone that facilitates to select an agrothecnical system that guarantees the increment and quality in the production of sweet potato in a continuous way in the municipality of Trinidad. The work was carried out in the Grange: Valle de los Ingenios Company of Agricultural FNTA, On an Alluvial floor of sandy slight texture and lightly flat topography, have being evaluated six sweet potatoes clones coming from the INIVIT, all commercial ones, for it was designed it an experiment in Latin Square with the clones: CEMSA 78-354 as witness, CEMSA 74-228, INIVIT B-98-2, INIVIT B-98-3, cautillo and INVIT 2005. It happens the same in the not very rainy time of November to April and rainy of May to November, The clones INIVIT 98-2 and INIVIT 2005 were the best for the not very rainy time and INIVIT 98-2 and CEMSA 74-228 for the rainy one; superiors to the witness CEMSA 78-354 and there was evidenced that in the planted area in the not very rainy time the losses are bigger for Tetuán in all of the clones.

  4. Modeling of mass transfer performance of hot-air drying of sweet potato (Ipomoea Batatas L. slices

    Directory of Open Access Journals (Sweden)

    Zhu Aishi

    2014-01-01

    Full Text Available In order to investigate the transfer characteristics of the sweet potato drying process, a laboratory convective hot air dryer was applied to study the influences of drying temperature, hot air velocity and thickness of sweet potato slice on the drying process. The experimental data of moisture ratio of sweet potato slices were used to fit the mathematical models, and the effective diffusion coefficients were calculated. The result showed that temperature, velocity and thickness influenced the drying process significantly. The Logarithmic model showed the best fit to experimental drying data for temperature and the Wang and Singh model were found to be the most satisfactory for velocity and thickness. It was also found that, with the increase of temperature from 60 to 80°C, the effective moisture diffusion coefficient varied from 2.962×10-10 to 4.694×10-10 m2×s-1, and it fitted the Arrhenius equation, the activation energy was 23.29 kJ×mol-1; with the increase of hot air velocity from 0.423 to 1.120 m×s-1, the values of effective moisture diffusion coefficient varied from 2.877×10-10 to 3.760×10-10 m2•s-1; with the increase of thickness of sweet potato slice from 0.002 m to 0.004 m, the values of effective moisture diffusion coefficient varied from 3.887×10-10 to 1.225×10-9 m2•s-1.

  5. Variability of sugars in staple-type sweetpotato (Ipomoea batatas) cultivars: The effects of harvest time and storage

    Science.gov (United States)

    Total soluble sugar content and composition was studied by high performance liquid chromatography in four high dry-matter sweet potato cultivars at 3, 4, and 5 months maturity. Total soluble sugar consisted of sucrose, glucose, and fructose, ranging from 4.10–10.82 g/100 g (dry-weight basis). At har...

  6. Immunomodulatory effects of Alliums and Ipomoea batata extracts on lymphocytes and macrophages functions in White Leghorn chickens: in vitro study.

    Science.gov (United States)

    Hanieh, Hamza; Narabara, Kiyoaki; Tanaka, Yuji; Gu, Zhigang; Abe, Asaki; Kondo, Yasuhiro

    2012-01-01

    We previously described that supplementary garlic, onion and purple sweet potato (PSP) enhance humoral immune response in White Leghorn chickens. In the present in vitro study, we investigated the effects of garlic (GE), onion (OE) and PSP (PSPE) extracts on proliferation, interleukin (IL)-2 and interferon (INF)-γ gene expression of stimulated lymphocytes. The effects on microbicidal activity, reactive oxygen species (ROS) and nitric oxide (NO) productions of stimulated peritoneal macrophages were studied as well. The results showed that GE augmented Concanavalin A (ConA)-induced splenocytes (4, 8 and 16µg/mL) and thymocytes (2, 4 and 8µg/mL) proliferations, and gene expression of IL-2 (8 and 16µg/mL) and INF-γ (16µg/mL). None of the examined extracts had mitogenic effect nor stimulated bursacytes response to phorbol 12-myristate 13-acetate (PMA). Macrophages exhibited superior microbicidal activity and ROS production with GE at 4 and 8µg/mL and with OE at 25.6µg/mL. None of the extracts showed stimulatory effects on NO production. The extracts showed concentration-dependent inhibitory effects on all measured parameters at higher concentrations. Taken together, it is likely that garlic has direct stimulatory effects on immune cell functions, whereas the in vitro inhibitory effects of onion and PSP were likely attributed to high flavonoid contents.

  7. Toxic Ipomeamarone accumulation in healthy parts of Sweetpotato (Ipomoea batatas L. Lam) storage roots upon infection by Rhizopus stolonifer.

    Science.gov (United States)

    Wamalwa, Lydia N; Cheseto, Xavier; Ouna, Elizabeth; Kaplan, Fatma; Maniania, Nguya K; Machuka, Jesse; Torto, Baldwyn; Ghislain, Marc

    2015-01-14

    Furanoterpenoid accumulation in response to microbial attack in rotting sweetpotatoes has long been linked to deaths and lung edema of cattle in the world. However, it is not known whether furanoterpenoid ipomeamarone accumulates in the healthy-looking parts of infected sweetpotato storage roots. This is critical for effective utilization as animal feed and assessment of the potential negative impact on human health. Therefore, we first identified the fungus from infected sweetpotatoes as a Rhizopus stolonifer strain and then used it to infect healthy sweetpotato storage roots for characterization of furanoterpenoid content. Ipomeamarone and its precursor, dehydroipomeamarone, were identified through spectroscopic analyses, and detected in all samples and controls at varying concentrations. Ipomeamarone concentration was at toxic levels in healthy-looking parts of some samples. Our study provides fundamental information on furanoterpenoids in relation to high levels reported that could subsequently affect cattle on consumption and high ipomeamarone levels in healthy-looking parts.

  8. IPOMOEA BATATAS SYRUP DECREASE MALONDIALDEHYDE AND INCREASE NITROUS OXIDE PLASMA LEVELS AMONGST MODERATE SMOKER WORKERS AT DENPASAR

    Directory of Open Access Journals (Sweden)

    I Dewa Ayu Intan Dwi-Primayanti

    2012-09-01

    Full Text Available Objective: Cells and tissues are continuously damaged by reactive oxygen species. Cigarette smoke is one of an exogenous source of free radical containing more than 4000 chemical compounds, that triggering the formation of free radicals related to diseases and aging process. Anthocyanins are potent antioxidants that are widely distributed in fruit, vegetables, red wines and Ipomea batatas. The aim of this study was to determine the effect of Ipomea batatas as a source of antioxidants in decreasing levels of alondialdehyde (MDA and increasing of Nitrous oxide (nitrite/nitrate/NOxplasma in moderate smokers of workers at Denpasar.Method: This was an experimental study with a pretest-posttest control groups design. There are 33 moderate smokers who were divided into three groups, control group (placebo, treatment group with 15 ml purple sweet potato syrup (P1 and 30 ml (P2, for 14 days. All groups were performed the laboratory examinations for MDA and NOx plasma before and after the treatment.Results: This study showed that there were significant differences (p<0.05 both in MDA and NOx plasma levels in the control group, P1 and P2. The decrease of MDAlevels on P1 was 35.39% and on P2 was 49.87%. The increase of NOx plasma levels was 7.78% for P1 and 14.68% for P2.Conclusion: From this study, it can be concluded that Ipomea batatas syrup contains of 8mg/mL anthocyanins, probably play a role in reducing the free radical and thus reducingthe risk of disease and slowing the aging process.

  9. 五爪金龙、三裂叶薯和七爪龙水浸液对4种作物种子萌发与幼苗生长的影响%Effect of Aqueous Extracts of 3 Plants (Ipomoea cairica, Ipomoea triloba and Ipomoea digitata) on Seed Germination and Seedling Growth of Radish, Cabbage, Mustard and Asparagus Lettuce

    Institute of Scientific and Technical Information of China (English)

    宋鑫; 沈奕德; 黄乔乔; 李晓霞; 范志伟

    2013-01-01

    采用培养皿滤纸法,研究了强入侵植物五爪金龙、弱入侵植物三裂叶薯和海南本地植物七爪龙叶片水浸液对萝卜、白菜、芥菜和莴笋等4种作物的种子萌发与幼苗生长的影响.结果表明,五爪金龙地上叶片水浸液对4种作物种子萌发和幼苗生长都具有明显的抑制作用,且随着质量浓度的增大,抑制作用增强,低质量浓度(0.1 g· mL-1)的水浸液对白菜、莴笋、芥菜、萝卜幼苗根的抑制率分别为81.3%,80.1%,74.2%和64.8%,高质量浓度(0.5g·mL-1)的水浸液可以使各受体幼苗停止生长.七爪龙和三裂叶薯叶片水浸液为低质量浓度时促进种子生长,高质量浓度时对根具有轻微的抑制作用.高质量浓度的七爪龙叶片水浸液对白菜、莴笋、芥菜和萝卜幼苗根的抑制率分别为47.7%,59.3%,43.6%和22.8%.高质量浓度的三裂叶薯叶片水浸液对白菜、莴笋、芥菜和萝卜幼苗根的抑制率分别为78.7%,75%,49.6%和25.1%.此外,五爪金龙具有较强的化感作用,且其化感作用大于三裂叶薯和七爪龙.

  10. 一种净化水质的复合生物修复系统%A composite bioremediation system for water purification

    Institute of Scientific and Technical Information of China (English)

    袁亚光; 张弛; 谢卫平; 赵福庚; 钦佩

    2012-01-01

    在实验室内利用人工模拟方法,选择水蕹菜(Ipomoea aquatica)、泥鳅(Misgurus anguillicaudatus)、沼泽红假单胞菌(Rhodopseudomonas palustris)为工程物种,构建一套水生经济植物-水生动物-微生物复合生物修复系统进行污水修复,研究该系统中动植物生物量及水质指标的变化.结果表明,在23 d的实验周期中,水体铵态氮(NH4+-N)下降96.5%,硝态氮(NO3--N)下降82.2%,总磷(TP)下降53.2%,化学需氧量(CODMn)下降24.5%.水蕹菜平均增重31.2%,泥鳅平均增重6.1%.这种复合的生物修复模式具有较好的经济效益与环境效益.%By the method of artificial simulation in laboratory, and with water spinach (Ipomoea aquatic), loach (Misgurus anguillkaudatus) , and a kind of pseudomonad (Rhodopseudomonas palustris) as the engineering species, a composite bioremediation system of aquatic economic plant - aquatic animal - microorganism was constructed to improve the water quality of sewage. The biomass of I. aquatic and M. anguillicaudatus in the system and the water quality indices were investigated. Within the 23 days experimental period, the nutrient concentrations in the sewage decreased significantly, with the removal rates of NH4+-N, NO3--N, TP, and CODMn being 96.5%, 82. 2%, 53. 2%, and 24.5%, respectively. Meanwhile, the biomass of I. aquatic and M. anguillicaudatus was averagely increased by 31.2% and 6. 1% , respectively. All the results suggested that this composite bioremediation model had good economic and environmental benefits.

  11. 蕹菜镉积累品种根际土壤水溶性有机质的结构特征研究%Structural Characteristics of Dissolved Organic Matter in the Rhizosphere Soil of Water Spinach Cultivars Differing in Cd Accumulation

    Institute of Scientific and Technical Information of China (English)

    龚玉莲; 杨中艺; 陈爱葵; 曾小龙; 曾碧健; 王平洁; 汤域巍

    2015-01-01

    Fourier transform infrared spectroscopy (FTIR)and nuclear magnetic resonance spectroscopy (1 H NMR and 13 C NMR)were used to investigate the structural characteristics of dissolved organic mat-ter (DOM)in the rhizosphere soil of a low-Cd cultivar (QLQ)and a high-Cd cultivar (T308)of water spinach (Ipomoea aquatica Forsk.).The results showed that carbohydrates were the main components of DOMin the rhizosphere soil of the two cultivars,and the contents of alkyl and aromatic substances were relatively low.There were obvious differences in the structural characteristics of DOM between the two cultivars.The relative contents of carboxylic C and long-chain aliphatic C of DOMin the rhizosphere soil of QLQ were lower than those of T308,but that of aromatic substance of QLQ was higher.Alkyl sub-stance of DOM in the rhizosphere soil of QLQ possessed the characteristics of relatively high content, short branched chain and high branched chain.Although carbohydrate H content of DOM in the rhizo-sphere soil of QLQ was lower than that of T308,carbohydrate C content was higher than that of T308, which suggested that there might be differences in the structure of carbohydrates of DOM in the rhizo-sphere soil between the cultivars.The results indicated that the structural characteristics of DOM in the rhizosphere soil of the low-Cd cultivar might influence Cd behavior and bioavailability,leading to relative-ly low ability to activate soil Cd,which would be associated with its low Cd accumulation.%采用 FTIR 和1 H NMR、13 C NMR 研究了蕹菜 Ipomoea aquatica Forsk.低 Cd 品种(QLQ)和高 Cd 品种(T308)根际土壤水溶性有机质(dissolved organic matter,DOM)的结构特征。结果显示蕹菜两个品种根际土壤DOM均以碳水化合物为主要组成物质,烷基链烃和芳香族物质较少。两个品种根际土壤 DOM的结构特征存在明显差异。QLQ 根际土壤 DOM中羧基 C 和长链脂肪 C 的相对含量较 T308低

  12. Strategy and scenario for wetland conservation in India

    Directory of Open Access Journals (Sweden)

    Monjit Paul

    2011-01-01

    Full Text Available Wetlands are the most important ecosystems for the organisms in Animal Kingdom (including human beings and Plant Kingdom. There are about hundred species of flora in and around Indian Wetlands. They include Sagittaria montividensis, Cryptocoryne ciliata, Cyperus spp., Acrostichum aureum, Ipomoea aquatica, etc. They are also the habitats of several mammals like the marsh mongoose, small Indian mongoose, palm civet and the small Indian civet. Endangered species like the Indian mud turtle have also been found in the wetlands. Certain species of birds also visit the wetlands. Prominent ones are grebe, coot, darter, shag, cormorant, teals, egrets, jacanas, snipes, tern, eagle, sand piper, gulls, rails and kingfishers. The wetlands are important for production of foods and human safety. The East Kolkata wetlands with their garbage farms and fishponds have provided the city with three facilities, i.e., food, sanitation and livelihood. They also provide ecological security to the city of Kolkata. Over the past few years, wetlands have come under severe threat. With the population explosion, some of the largest fish farms have been converted from pisiculture to paddy cultivation. Industries also empty their wastewater effluent without treatment to the channels flowing eastward and these ultimately land up in the wetlands. This has caused substantial amount of deposits of metal in the canal sludge and made the wastewater incapable for the consumption by the fishes and the plants grown in the wetland. Nevertheless, due to urbanization or human interference, the wetland and its unique ecosystem biodiversity are in danger. After Ramsar Convention, 1971, different acts have been passed in India for conservation of wetlands, along with conducting general awareness program for the local people by the government, conducting different programs, management of wetlands, and research by the government, NGOs and other institutions.

  13. Microcosm wetlands for wastewater treatment with different hydraulic loading rates and macrophytes.

    Science.gov (United States)

    Jing, Shuh-Ren; Lin, Ying-Feng; Wang, Tze-Wen; Lee, Der-Yuan

    2002-01-01

    Constructed wetlands (CW) usually require large land areas for treating wastewater. This study evaluated the feasibility of applying CW with less land requirement by operating a group of microcosm wetlands at a hydraulic retention time (HRT) of less than 4 d in southern Taiwan. An artificial wastewater, simulating municipal wastewater containing 200 mg L(-1) of chemical oxygen demand (COD), 20 mg L(-1) of NH4+-N (AN), and 20 mg L(-1) of PO4(3-)-P (OP), was the inflow source. Three emergent plants [reed, Phragmites australis (Cav.) Trin. ex Steud.; water primrose, Ludwigia octovalvis (Jacq.) P.H. Raven; and dayflower, Commelina communis L.] and two floating plants [water spinach, Ipomoea aquatica Forssk.; and water lettuce, Pistia stratiotes L.] plants were tested. The planted systems showed more nutrient removal than unplanted systems; however, the type of macrophytes in CW did not make a major difference in treatment. At the HRTs of 2 to 4 d, the planted system maintained greater than 72,80, and 46% removal for COD, AN, and OP, respectively. For AN and OP removal, the highest efficiencies occurred at the HRT of 3 d, whereas maximum removal rates for AN and OP occurred at the HRT of 2 d. Both removal rates and efficiencies were reduced drastically at the HRT of 1 d. Removals of COD, OP, and AN followed first-order reactions within the HRTs of 1 to 4 d. The efficient removals of these constituents obtained with HRT between 2 and 4 d indicated the possibility of using a CW system for wastewater treatment with less land requirement.

  14. Nutritional quality and safety aspects of wild vegetables consume in Bangladesh

    Institute of Scientific and Technical Information of China (English)

    Miah Mohammed Abdus Satter; Mohammed Murtaza Reza Linkon Khan; Syeda Absha Jabin; Nusrat Abedin; Mohammed Faridul Islam; Badhan Shaha

    2016-01-01

    Objective: To evaluate the nutritional composition, including major minerals, essential trace elements and toxic heavy metals of five different wild vegetables Dhekishak(Dryopteris filix-mas), Helencha(Enhydra fluctuans), Kalmishak(Ipomoea aquatica),Patshak(Corchorus capsularis) and Shapla stem(Nymphaea stellata) and their safety aspects.Methods: Proximate parameters moisture, ash, fat, fiber, protein, carbohydrate and energy; major minerals Na, K, Ca and Mg; trace elements Fe, Zn and Cu; and toxic heavy metals Pb, Cd, Cr, Ni and Hg were evaluated in the selected wild vegetables using the standard food analysis techniques.Results: The results from nutritional analysis showed that all the wild vegetables used in this study had a low content of crude fat and high content of moisture, ash, crude protein,crude fiber, carbohydrate and energy having the recommended dietary allowances. The vegetables were also rich in major minerals Na, K, Ca and Mg, sufficient in essential trace elements Fe, Cu and Zn while the heavy metals Pb, Cr and Ni were detected higher in amount in all the vegetables except Patshak than the limits recommended by Food and Agriculture Organization/World Health Organization. The heavy metals Cd and Hg were not detected in any vegetable.Conclusions: The outcome of this study suggests that the wild vegetables have very good nutritional potential to meet the recommended dietary allowances, but special awareness should be taken for public health concern about the high level of Pb, Cr and Ni which exceed the Food and Agriculture Organization/World Health Organization recommended limits for the metals in vegetables.

  15. Uptake and transport of roxarsone and its metabolites in water spinach as affected by phosphate supply.

    Science.gov (United States)

    Yao, Lixian; Li, Guoliang; Dang, Zhi; Yang, Baomei; He, Zhaohuan; Zhou, Changmin

    2010-04-01

    Roxarsone (ROX) is widely used as a feed additive in intensive animal production. While an animal is fed with ROX, the As compounds in the manure primarily occur as ROX and its metabolites, including arsenate (As[V]), arsenite (As[III]), monomethylarsonic acid (MMA), and dimethylarsinic acid (DMA). Animal manure is commonly land applied with phosphorous fertilizers in China. A pot experiment was conducted to investigate the phytoavailability of ROX, As(V), As(III), MMA, and DMA in water spinach (Ipomoea aquatica), with the soil amended with 0, 0.25, 0.50, 1.0, and 2.0 g PO(4)/kg, respectively, plus 2% (w/w manure/soil) chicken manure (CM) bearing ROX and its metabolites. The results indicate that this species of water spinach cannot accumulate ROX and MMA at detectable levels, but As(V), As(III), and DMA were present in all plant samples. Increased phosphorous decreased the shoot As(V) and As(III) in water spinach but did not affect the root As(V). The shoot DMA and root As(III) and DMA were decreased/increased and then increased/decreased by elevated phosphorous. The total phosphorous content (P) in plant tissue did not correlate with the total As or the three As species in tissues. Arsenate, As(III), and DMA were more easily accumulated in the roots, and phosphate considerably inhibited their upward transport. Dimethylarsinic acid had higher transport efficiency than As(V) and As(III), but As(III) was dominant in tissues. Conclusively, phosphate had multiple effects on the accumulation and transport of ROX metabolites, which depended on their levels. However, proper utilization of phosphate fertilizer can decrease the accumulation of ROX metabolites in water spinach when treated with CM containing ROX and its metabolites.

  16. Monitoring of arsenic in aquatic plants, water, and sediment of wastewater treatment ponds at the Mae Moh Lignite power plant, Thailand.

    Science.gov (United States)

    Nateewattana, Jomjun; Trichaiyaporn, Siripen; Saouy, Maliwan; Nateewattana, Jintapat; Thavornyutikarn, Prasak; Pengchai, Petch; Choonluchanon, Somporn

    2010-06-01

    Mae Moh is a risky area for arsenic contamination caused by the effluent from biowetland ponds in Mae Moh lignite-fuelled power plant. The objective of this study was to investigate the arsenic concentrations of Mae Moh biowetland ponds and determine the main factors which are important for arsenic phytoremediation in the treatment system. The result revealed that arsenic concentrations in the supernant were in the range of less than 1.0 microg As L(-1) to 2.0 microg As L(-1) while those in the sediment were in the range of 25-200 microg As kg soil(-1). Both values were below the Thailand national standard of 0.25 mg As L(-1) for water and 27 mg As kg soil(-1) for the soil. Arsenic accumulation in the biomass of 5 aquatic plants at the biowetland ponds ranged from 123.83 to 280.53 mg As kgPlant(-1). Regarding the result of regression analysis (R (2) = 0.474 to 0.954), high concentrations of organic matter and other soluble ions as well as high pH value in the sediment could significantly enhance the removal of soluble arsenic in the wetland ponds. From the regression equation of accumulated arsenic concentration in each aquatic plant, Eichhornia crassipes (Mart.) Solms. (R (2) = 0.954), Ipomoea aquatica Forsk. (R (2) = 0.850), and Typha angustifolia (L.) (R (2) = 0.841) were found to be preferable arsenic removers for wastewater treatment pond in the condition of low Eh value and high content of solid phase EC and phosphorus. On the other hand, Canna glauca (L.) (R (2) = 0.749) appeared to be favorable arsenic accumulator for the treatment pond in the condition of high Eh value and high concentration of soluble EC.

  17. Dynamique de la flore et de la végétation des Niayes et du Bassin arachidier au Sénégal

    Directory of Open Access Journals (Sweden)

    Elhadji Faye

    2014-12-01

    Full Text Available Reactualisation of floristic and phytosociologic datas in Niayes and Peanut-Basin areas were undertook with village scale approach to better underline resources' conervation. In Savannah and sub-guinean areas specially in the Peanut-Basin and Niayes, 288 floristic samples were put in place in 6 villages in an stratified way based on ethnic groups, land use systems and topography. In the same time, 25 samples were realised in the 2 reference sites (Noflaye and Sambandé and comparison were made with researches conducted in 1940. Datas were submitted to multivariate analysis. SØrensen similarity index were used to compare villages systems and natural sites. Results showed discrimination of 11 phytosociologic groups: 5 of which came from natural sites (savanah G4 Hexalobus monopetalus et Gardenia ternifolia and G8 Acacia macrostachya et Ischaemum rugosum, secondary forest G10 Aphania senegalensis et Voaganca africana, semi-aquatic vegetations G1 Ipomoea aquatica et I. dichroa and G5 Phragmites australis subsp australis et Paspalum vaginatum and 6 from anthropic systems (disturbed areas G6 Echinochloa colona et Jussiae erecta and G7 Dactyloctenium aegyptium et Brachiaria disticophylla, and finally post-cultural zones with G2 Brachiaria disticophylla et Cenchrus biflorus, G3 Celosia trigyna et Digitaria velutina, G9 Tephrosia purpurea et Cenchrus biflorus and G11 Mitracarpus scaber et Eragrostis tremula. Natural sites groups were progressively invided by disturded area species and the others by ruderals and nitrophyl species. Overall floristic richness is about 336 species among which 260 from the Niayes sites and 176 from Peanut-Basin ones. References sites are always richer in terms af genera but peul areas are richer in termes of species. Differences were evidenced between actual taxa and Trochain (1940 descriptions. This methodological approach might be extended to other ecogeographical zones in Senegal in order to better identify and follow

  18. Influence of nutrient level on methylmercury content in water spinach.

    Science.gov (United States)

    Greger, Maria; Dabrowska, Beata

    2010-08-01

    Widely consumed vegetables are often cultivated in sewage waters with high nutrient levels. They can contain high levels of methylmercury (MeHg), because they can form MeHg from inorganic Hg in their young shoots. We determined whether the MeHg uptake and the MeHg formation in the shoots of water spinach (Ipomoea aquatica) were affected by the presence of a high nutrient level in the growth medium. Water spinach shoots were rooted and pretreated in growth medium containing 7% (low) or 70% (high) Hoagland nutrient solution; thereafter, the plants were treated with either 0.02 microM MeHg or 0.2 microM HgCl2 for 3 d. Half the plants were then analyzed for total Hg and MeHg. The remaining plants were transferred to mercury-free medium with low or high nutrient levels and posttreated for 3 days before analysis of total Hg and MeHg in order to measure MeHg formation in the absence of external Hg. The results indicate that nutrient level did not influence MeHg uptake, but that a high nutrient level reduced the distribution of MeHg to the shoots 2.7-fold versus low nutrient level. After treatment with HgCl2, MeHg was found in roots and new shoots but not in old shoots. The MeHg:total-Hg ratio was higher in new shoots than in roots, being 13 times higher at high versus low nutrient levels. Thus, MeHg formation was the same in new shoots independent of inorganic Hg concentration, since the total Hg level decreased at a high nutrient level.

  19. Detection of Ni, Cd, and Cu in green leafy vegetables collected from different cultivation areas in and around Colombo District, Sri Lanka.

    Science.gov (United States)

    Kananke, Thilini; Wansapala, Jagath; Gunaratne, Anil

    2016-03-01

    Five types of green leafy vegetables ["Kankun" (Ipomoea aquatica), "Mukunuwenna" (Alternanthera sessilis), "Thampala" (Amaranthus viridis), "Nivithi" (Basella alba), and "Kohila leaves" (Lasia spinosa)] were randomly collected from six different locations (Wellampitiya, Kolonnawa, Kottawa, Piliyandala, Bandaragama, and Kahathuduwa) in and around Colombo District, Sri Lanka, and subjected to analysis of three heavy metals [nickel (Ni), cadmium (Cd), and copper (Cu)] by atomic absorption spectrometry. Soils from green leafy vegetable cultivation lands and irrigated water were also tested. The average concentrations of heavy metals Ni, Cd, and Cu in soils were 51.5 ± 45.5, 1.4 ± 1.1, and 66.5 ± 59.5 mg kg(-1), respectively. The highest Ni contamination was detected in the irrigated water samples from Wellampitiya (2.02 mg L(-1)) followed by Kolonnawa (1.02 mg L(-1)) and Kahathuduwa (0.25 mg L(-1)) areas. This has exceeded the WHO/FAO guideline (0.2 mg L(-1)). However, Cd and Cu contents in all tested irrigated water samples were well below the detection limits. Significant differences were observed in Ni, Cd, and Cu levels, between both production sites and green leafy vegetables analyzed (P vegetable samples collected from six areas varied as 0.23 ± 0.15 for Cd, 12.60 ± 9.01 for Cu, and 7.62 ± 8.41 for Ni. Maximum Ni, Cd, and Cu contaminations were found in the green leafy vegetables collected from Kolonnawa area. Among the green leafy vegetables analyzed, "Kohila leaves" have the highest tendency to accumulate Ni, Cd, and Cu from the environment.

  20. [Effects of phosphorus application on translocation of lead, zinc and cadmium in the soil-plant system].

    Science.gov (United States)

    Wang, Bi-Ling; Xie, Zheng-Miao

    2008-11-01

    A pot experiment was conducted to determine the influence of phosphorus (P) application on the translocation and accumulation of lead (Pb), zinc (Zn), and cadmium (Cd) in a soil solid-liquid-plant system using a plant Swamp Morningglory (Ipomoea aquatica Forak) as an indicator and Visual MINTEQ model. A contaminated soil containing 22,830 mg x kg(-1) Pb, 746 mg x kg(-1) Zn, and 10 mg x kg(-1) Cd was in the root was significantly decreased after P application, while the Zn concentration were increased, but no impact on Cd concentration after P application, compared to the control. Further more, concentrations of Pb, Zn, and Cd in plant tissue were sharply reduced after P application by 53%-92%, 35%-71%, and 59%-86%, respectively; Concentrations decreased exponentially with increasing amount of P; Relationship between Pb and Zn (r = 0.993, p < 0.01), between Pb and Cd (r = 0.986, p < 0.01) suggested that there was a positive relationship between the mechanisms controlling the phytoavailability of Pb, Zn, and Cd. It was suggested that Cd concentrations in the soil solution were reduced generally by P application as a result of the increased soil pH, and Zn concentrations were no change, but Pb concentrations were increased. Species distribution showed that PbHPO4, PbOH+ and PbH2PO4+ were the main complexation species of Pb in the soil equilibrium solution after P application. It was also suggested that Pb accumulation by root were negatively correlated to Pb in soil solution with coefficient of -0.872 (p < 0.05), and that water soluble Zn and Cd concentrations were reduced while the concentrations in root tissues were increased after P addition. These results indicate the difference between Zn, Cd and Pb and suggest that P as KH2PO4 successfully decreased the phytotoxicity of Pb, Zn, and Cd in the contaminated soil.

  1. The influence of aquatic humic substances characteristics on the coagulation efficiency using ferric chloride; Influencia das caracteristicas das substancias humicas aquaticas na eficiencia da coagulacao com o cloreto ferrico

    Energy Technology Data Exchange (ETDEWEB)

    Sloboda, Eliane; Vieira, Eny Maria [Universidade de Sao Paulo (USP), Sao Carlos, SP (Brazil). Inst. de Quimica], e-mail: elisloboda@hotmail.com; Dantas, Angela Di Bernardo; Bernardo, Luiz Di [Universidade de Sao Paulo (USP), Sao Carlos, SP (Brazil). Escola de Engenharia. Dept. de Hidraulica e Saneamento

    2009-07-01

    The aim of this study was to verify the influence of the apparent molecular size of aquatic humic substances on the effectiveness of coagulation with ferric chloride. Coagulation-filtration tests using jar test and bench-scale sand filters were carried out on samples of water with true color of approximately 100 Hazen units, prepared with aquatic humic substances of different molecular sizes (F{sub 1}: < 0.45 {mu}m, F{sub 2}: 100 kDa - 0.45 {mu}m, F{sub 3}: 30 - 100 kDa and F{sub 4}{sup '} : < 30 kDa). For the water samples with lower apparent molecular size fractions, greater dosages of coagulant was needed to remove the color around 5.0 Hanzen units, mainly because these water samples contain higher concentrations of fulvic acids, which exhibited a larger number of negatively-charged groups. (author)

  2. NCBI nr-aa BLAST: CBRC-DNOV-01-0322 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-0322 dbj|BAB16380.1| Na+/H+ exchanger [Ipomoea nil] dbj|BAB16381.1| Na+/H+ exchanger [Ipomoea... nil] dbj|BAB60899.1| Na+/H+ exchanger protein [Ipomoea nil] BAB16380.1 0.21 26% ...

  3. CARACTERIZACIÓN ANATÓMICA DEL DESARROLLO DE LA RAÍZ RESERVANTE DE CAMOTES PERUANOS (IPOMOEA BATATAS (L. LAM., CONVOLVULACEAE

    Directory of Open Access Journals (Sweden)

    M. Marín

    2014-06-01

    Full Text Available La caracterización anatómica del desarrollo de la raíz reservante fue llevada a cabo en tres genotipos de camotes. En los estadios iniciales se encontraron diferencias en el desarrollo del xilema primario, incluyendo la formación de un parénquima medular. La actividad cambial secundaria contribuyó en gran medida al desarrollo del parénquima reservante, determinando un arreglo diferencial de los haces vasculares. Así mismo, fueron hallados granos simples y compuestos de almidón, siendo las dimensiones de los primeros asociadas a las mayores dimensiones celulares del parénquima. Uno de los genotipos evaluados presentó altos valores en el espesor del súber y granos de almidón (123,9 µm y 29,5 µm respectivamente. Se sugiere usar estas características como parámetros en la selección de genotipos de camote.

  4. Balance energético de dos aperos de labranza en un Fluvisol para el cultivo del boniato (Ipomoea batatas Lam

    Directory of Open Access Journals (Sweden)

    Yosvel Enrique Olivet Rodríguez

    2013-01-01

    Full Text Available El trabajo de investigación se llevó a cabo en la Granja Agropecuaria “Ranulfo Leyva Pacheco” perteneciente a la Empresa de Cultivos Varios “Paquito Rosales Benítez”. El proceso de preparación de suelo para el cultivo del boniato no solo altera las propiedades físicas del suelo sino que también conlleva a un elevado consumo energético, especialmente la labor de rotura con arado de discos, donde se consume un gran porciento de combustible. El objetivo de este trabajo ha sido evaluar un apero de labranza alternativo de corte vertical y horizontal al tradicional con arado de discos en la labor de rotura, que permita reducir el consumo energético del proceso bajo las condiciones de un Fluvisol y las emisiones de dióxido de carbono que llegan a la atmósfera. Los tratamientos considerados han sido rotura tradicional del suelo, T1, que consistió en romper el suelo con arado de discos (ADI-3M y rotura del suelo, T2, que consistió en romper el suelo con multiarado. Presentando este último el menor consumo energético de uso directo (0,83 GJ ha-1 e indirecto (0,08 GJ ha-1, con un consumo total de 0,91 GJ ha-1, ocasionando la reducción del 66% de las emisiones de CO2, motivado por una reducción del 69% del consumo de combustible (20,2 L ha-1 con relación a la rotura con el arado de discos con 29,2 L ha-1, bajo las mismas condiciones de suelo y clima.

  5. Accumulation of lead and arsenic in Malabar spinach (Basella alba L.) and sweet potato (Ipomoea batatas L.) leaves grown on urban and orchard soils.

    Science.gov (United States)

    Migration of people of different ethnic backgrounds to U.S. urban areas has resulted in different ethnic vegetable crops being grown in urban gardens. There are concerns that some ethnic vegetable crops may accumulate heavy metals when grown on urban soils. The objective of this study was to evalua...

  6. Water-Deficit Tolerance in Sweet Potato [Ipomoea batatas (L. Lam.] by Foliar Application of Paclobutrazol: Role of Soluble Sugar and Free Proline

    Directory of Open Access Journals (Sweden)

    Suravoot Yooyongwech

    2017-08-01

    Full Text Available The objective of this study was to elevate water deficit tolerance by improving soluble sugar and free proline accumulation, photosynthetic pigment stabilization, photosynthetic abilities, growth performance and storage root yield in sweet potato cv. ‘Tainung 57’ using a foliar application of paclobutrazol (PBZ. The experiment followed a Completely Randomized Block Design with four concentrations of PBZ: 0 (control, 17, 34, and 51 μM before exposure to 47.5% (well irrigation, 32.3% (mild water deficit or 17.5% (severe water deficit soil water content. A sweet potato cultivar, ‘Japanese Yellow’, with water deficit tolerance attributes was the positive check in this study. Total soluble sugar content (sucrose, glucose, and fructose increased by 3.96-folds in ‘Tainung 57’ plants treated with 34 μM PBZ grown under 32.3% soil water content (SWC compared to the untreated plants, adjusting osmotic potential in the leaves and controlling stomatal closure (represented by stomatal conductance and transpiration rate. In addition, under the same treatment, free proline content (2.15 μmol g-1 FW increased by 3.84-folds when exposed to 17.5% SWC. PBZ had an improved effect on leaf size, vine length, photosynthetic pigment stability, chlorophyll fluorescence, and net photosynthetic rate; hence, delaying wilting symptoms and maintaining storage root yield (26.93 g plant-1 at the harvesting stage. A positive relationship between photon yield of PSII (ΦPSII and net photosynthetic rate was demonstrated (r2 = 0.73. The study concludes that soluble sugar and free proline enrichment in PBZ-pretreated plants may play a critical role as major osmoprotectant to control leaf osmotic potential and stomatal closure when plants were subjected to low soil water content, therefore, maintaining the physiological and morphological characters as well as storage root yield.

  7. Éxito reproductivo y calidad de los frutos en poblaciones del extremo sur de la distribución de Ipomoea purpurea (Convolvulaceae

    Directory of Open Access Journals (Sweden)

    Leonardo Galetto

    2002-01-01

    Full Text Available Se evaluó la producción de frutos y semillas en Ipomea purpurea en relación con diferentes tratamientos de polinización (autopolinización espontánea, autopolinización manual y polinización cruzada, durante dos temporadas reproductivas. La masa de los frutos, de las semillas y el número de semillas por fruto, se compararon entre tratamientos y entre años, a fin de detectar diferencias en la calidad de los frutos. Los resultados para las poblaciones estudiadas en el centro de Argentina confirman que se trata de una especie autocompatible y con la capacidad de producir frutos por autogamia espontánea. Los valores de éxito reproductivo mediados por polinización natural fueron altos (> 50%. Un fruto maduro pesa 0,1 g y contiene 4-6 semillas; la masa del total de semillas producidas por un fruto representa alrededor del 60% de la masa total del fruto. Los caracteres de los frutos y las semillas no presentaron diferencias significativas entre años o tratamientos. Asimismo, se compararon los frutos provenientes de polinización natural entre los individuos con distinto color de flor. En general, tampoco se encontraron diferencias significativas. Sólo los frutos provenientes de flores rosa presentaron un número significativamente menor de semillas que los frutos de los otros morfotipos florales (blanco o púrpura. El grupo principal de visitantes florales fue el de los Himenópteros, que pueden ser considerados polinizadores. Se registraron otros grupos de visitantes florales que no tendrían un papel preponderante en la transferencia de polen

  8. Chemical composition, nitrogen degradability and in vitro ruminal biological activity of tannins in vines harvested from four tropical sweet potato (Ipomoea batatas L.) varieties.

    Science.gov (United States)

    Ali, R; Mlambo, V; Mangwe, M C; Dlamini, B J

    2016-02-01

    This study investigated the potential of vines from four sweet potato varieties (Tia Nong 57, Tia Nong 66, Ligwalagwala and Kenya) as alternative feed resources for ruminant livestock. The chemical composition [neutral detergent fibre (NDF), acid detergent fibre (ADF), crude protein (CP) and acid detergent insoluble nitrogen (ADIN)], in vitro ruminal nitrogen (N) degradability and in vitro ruminal biological activity of tannins in the vines, harvested at 70 and 110 days after planting (DAP), were determined. Variety and harvesting stage did not (p > 0.05) influence CP and NDF content of the vines. Concentration of CP ranged from 104.9 to 212.2 g/kg DM, while NDF ranged from 439.4 to 529.2 g/kg DM across harvesting stages and varieties. Nitrogen degradability (ND) at 70 and 110 DAP was highest (p tannin-binding polyethylene glycol (PEG) increased (p tannins, as measured by increment in gas production parameters upon PEG inclusion, had a maximum value of 18.2%, suggesting low to moderate antinutritional tannin activity. Ligwalagwala vines, with highly degradable N, would be the best protein supplement to use during the dry season when ruminant animals consume low N basal diets and maintenance is an acceptable production objective. Tia Nong 66 and Kenya varieties, with less degradable N, may be more suitable for use as supplements for high-producing animals such as dairy goats.

  9. Role of non enzymatic synthesis of indole-3-acetic acid in the Ipomoea batatas L. Lam. (sweet potato) response to gamma radiation

    Energy Technology Data Exchange (ETDEWEB)

    Lage, C.L.S.; Esquibel, M.A. [Universidade Federal, Rio de Janeiro, RJ (Brazil). Inst. de Biofisica. Lab. de Fisiologia Vegetal

    1995-12-01

    Indolacetic acid (IAA) production by tryptophan radiolysis was evaluated after irradiation of a tryptophan solution (1.0 mg/ml) with a 2000 Gy dose of gamma rays followed by High Performance Liquid Chromatography (HPLC) analysis. New absorbance peaks at 280 nm were detected in the irradiated solution, one of them having a migration time similar to that of IAA, indicating a0.14% yield for the conversion reaction. The low yield led us to abandon the hypothesis that non-enzymatic IAA synthesis may account for growth simulation. When the electrophoretic patterns of peroxidases from absorbent roots derived from storage roots treated with IAA (1.0 mg/l) or gamma radiation were compared with control, differences were detected only in the irradiated material. Thus the growth radiation-induced effects do not seem to result from a sudden endogenous IAA increment. (author)

  10. Antioxidant Activity of Polyphenolic Extract from Ipomoea batatas L.%甘薯多酚粗提液的抗氧化活性研究

    Institute of Scientific and Technical Information of China (English)

    张赟彬; 龚钢明; 戴妙妙

    2007-01-01

    采用抗坏血酸和没食子酸作为对照,全面测定了甘薯多酚粗提液对超氧阴离子自由基、羟基自由基、DPPH自由基的清除率、还原能力、抗脂质过氧化活性以及对食用油脂的抗氧化性的影响,结果表明甘薯多酚粗提液具有较强的综合抗氧化能力,是一种有开发价值的天然抗氧化剂.

  11. Caracterización postcosecha y composición química de la batata (Ipomoea batatas (L. Lamb. variedad Topera.

    Directory of Open Access Journals (Sweden)

    Auris Damely García-Méndez

    2016-06-01

    Full Text Available El objetivo de este trabajo fue caracterizar la composición química y calidad postcosecha de la batata variedad Topera proveniente de una producción semi-mecanizada con buenas prácticas agrícolas (BPA. El estudio se realizó en el Laboratorio de Procesamiento Primario de Productos Agrícolas y Bioquímica de Alimentos de la Facultad de Agronomía (FAGRO de la Universidad Central de Venezuela (UCV durante el año 2014. La metodología permitió determinar algunas características físicas, texturales y composición química por los métodos de AOAC (Association of Official Analytical Chemists. Se obtuvieron batatas con un menor coeficiente de variabilidad en el peso (CV: 20,12%, forma (CV: 5,63% y tamaño (CV: 12,80%. Esta respuesta permitió establecer tres categorías de calidad con una baja incidencia de defectos (2,15% y daños físico-mecánicos (30,25%, esta última asociada a la alta resistencia del material (6,25+0,05 kgf/mm. Las batatas presentaron contenidos de azúcares reductores de 9,88%, fibra de 6,19% y proteína de 4,13%; estas características nutricionales sugieren su uso como suplemento alimenticio. La producción semi-mecanizada con la implementación de las técnicas de las BPA, permitió obtener batatas con características de importancia para la agroindustria procesadora de alimentos IV Gama y, a nivel del consumidor, con una calidad física homogénea.

  12. 非洲高淀粉甘薯品种Tanzania的再生研究%Somatic Embryogenesis of an Africa Sweet Potato (Ipomoea batatas (L. ) Lam. ) Variety

    Institute of Scientific and Technical Information of China (English)

    罗红蓉; 张大鹏; W.ROCA; 张义正

    2006-01-01

    研究了一个非洲高淀粉甘薯品种Tanzania的胚状体再生途径.将去腋芽的茎段在含有不同生长素浓度(0~2 mg/L)MS培养基上置于暗处,经过6~8周诱导出了胚性愈伤组织.Tanzania品种的胚性愈伤组织诱导频率较低,平均为13.4%,而对照Huachano品种为43.3%.Tanzania品种胚性愈伤组织由于生长素种类的不同而呈现不同的质量,形态和颜色特征.当诱导条件优化后,Tanzania品种茎尖在含1mg/L的4-fluorophenoxyacetic acid(4-FA)的培养基上,胚性愈伤组织诱导率可达50%.胚性愈伤组织在含1.0 mg/L的脱落酸(ABA)和1.0 mg/L的赤霉素(GA3)培养基上分化、发育,再转移至无激素的培养基上再生出完整植株.

  13. Diversity and evolutionary relationship of nucleotide binding site-encoding disease-resistance gene analogues in sweet potato (Ipomoea batatas Lam.)

    Indian Academy of Sciences (India)

    Guanshui Chen; Daren Pan; Yifei Zhou; Sheng Lin; Xiangde Ke

    2007-06-01

    Most plant disease-resistance genes (-genes) isolated so far encode proteins with a nucleotide binding site (NBS) domain and belong to a superfamily. NBS domains related to -genes show a highly conserved backbone of an amino acid motif, which makes it possible to isolate resistance gene analogues (RGAs) by degenerate primers. Degenerate primers based on the conserved motif (P-loop and GLPL) of the NBS domain from -genes were used to isolate RGAs from the genomic DNA of sweet potato cultivar Qingnong no. 2. Five distinct clusters of RGAs (22 sequences) with the characteristic NBS representing a highly diverse sample were identified in sweet potato genomic DNA. Sequence identity among the 22 RGA nucleotide sequences ranged from 41.2% to 99.4%, while the deduced amino acid sequence identity from the 22 RGAs ranged from 20.6% to 100%. The analysis of sweet potato RGA sequences suggested mutation as the primary source of diversity. The phylogenetic analyses for RGA nucleotide sequences and deduced amino acids showed that RGAs from sweet potato were classified into two distinct groups—toll and interleukin receptor-1 (TIR)-NBS-LRR and non-TIR-NBS-LRR. The high degree of similarity between sweet potato RGAs and NBS sequences derived from -genes cloned from tomato, tobacco, flax and potato suggest an ancestral relationship. Further studies showed that the ratio of non-synonymous to synonymous substitution within families was low. These data obtained from sweet potato suggest that the evolution of NBS-encoding sequences in sweet potato occur by the gradual accumulation of mutations leading to purifying selection and slow rates of divergence within distinct -gene families.

  14. Plastidial [alpha] -glucan phosphorylase 1 complexes with disproportionating enzyme 1 in Ipomoea batatas storage roots for elevating malto-oligosaccharide metabolism

    National Research Council Canada - National Science Library

    Yi-Chen Lin; Shih-Chung Chang; Rong-Huay Juang

    2017-01-01

    .... The immunohistochemistry analyses also revealed the co-localization of Pho1 and Dpe1 in the amyloplasts, and the protein levels of Pho1 and Dpe1 increased gradually throughout sweet potato storage root development...

  15. 甘薯(Ipomoea batatas(L.) Lam.)试管苗种质保存技术研究进展

    Institute of Scientific and Technical Information of China (English)

    张海燕; 张立明; 王庆美; 李爱贤; 孙立荣

    2005-01-01

    甘薯种质资源的大田种植保存面临着严重的病毒感染以及病虫害、自然灾害的影响。由茎尖分生组织培养成无病毒苗并进行试管保存,是当前既经济又实用的方法。

  16. Distribution of Phenolic Compounds and Antioxidative Activities in Parts of Sweet Potato (Ipomoea batata L.) plants and in home processed roots

    Science.gov (United States)

    We measured six phenolic compounds by HPLC, the total phenolic content by Folin-Ciocalteu, and antioxidative activities by three methods in the sweet potato plant and in home processed roots. Total phenolic content was highest in the leaves. Eight root varieties were partitioned and analyzed for p...

  17. 甘薯(Ipomoea batatas(L.)lam)不定根根尖细微结构的初步研究

    Institute of Scientific and Technical Information of China (English)

    戴大临

    1998-01-01

    顶端分生组织细胞有高的核质比,胸质中可见前质体和其它细胞,器。分化的组织原细胞中,皮层原细胞较中柱原和根冠表皮原细胞内前质体增多明显。

  18. The potential of computer vision, optical backscattering parameters and artificial neural network modelling in monitoring the shrinkage of sweet potato (Ipomoea batatas L.) during drying.

    Science.gov (United States)

    Onwude, Daniel I; Hashim, Norhashila; Abdan, Khalina; Janius, Rimfiel; Chen, Guangnan

    2017-07-30

    Drying is a method used to preserve agricultural crops. During the drying of products with high moisture content, structural changes in shape, volume, area, density and porosity occur. These changes could affect the final quality of dried product and also the effective design of drying equipment. Therefore, this study investigated a novel approach in monitoring and predicting the shrinkage of sweet potato during drying. Drying experiments were conducted at temperatures of 50-70 °C and samples thicknesses of 2-6 mm. The volume and surface area obtained from camera vision, and the perimeter and illuminated area from backscattered optical images were analysed and used to evaluate the shrinkage of sweet potato during drying. The relationship between dimensionless moisture content and shrinkage of sweet potato in terms of volume, surface area, perimeter and illuminated area was found to be linearly correlated. The results also demonstrated that the shrinkage of sweet potato based on computer vision and backscattered optical parameters is affected by the product thickness, drying temperature and drying time. A multilayer perceptron (MLP) artificial neural network with input layer containing three cells, two hidden layers (18 neurons), and five cells for output layer, was used to develop a model that can monitor, control and predict the shrinkage parameters and moisture content of sweet potato slices under different drying conditions. The developed ANN model satisfactorily predicted the shrinkage and dimensionless moisture content of sweet potato with correlation coefficient greater than 0.95. Combined computer vision, laser light backscattering imaging and artificial neural network can be used as a non-destructive, rapid and easily adaptable technique for in-line monitoring, predicting and controlling the shrinkage and moisture changes of food and agricultural crops during drying. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  19. Physical Characteristics of White Sweet Potato (Ipomoea batatas L.), Rice (Oryza sativa L.), and Tapioca (Manihot esculenta) Flours - Based Seasoning Composite Flour

    Science.gov (United States)

    Alfani, NNA; Ishartani, D.; Anam, C.; Praseptiangga, D.; Manuhara, G. J.

    2017-04-01

    The objective of this study was to determine the physical characteristics of seasoning composite flour that made from white sweet potato, rice, and tapioca flours, and determined the best formula of seasoning composite flour. A completely randomized design (CRD) with formula as the single factor was used. The data were analyzed by one-way ANOVA method and followed by Duncan Multiple Range Test (DMRT) at significance 5% if there was a significant difference. The best formula of seasoning composite flour was 30% tapioca flour, 30% rice flour, and 40% white sweet potato flour. The physical characteristics of the best formula were 5.689 ml/g of swelling power, 2.681 g/g of water absorption capacity, 0.887 ml/g of oil absorption capacity, and 22.03% cooking loss. Physical characteristics of the best seasoning composite flour were significantly different from the commercial seasoning flour and showed a better cooking loss, oil absorption capacity, and swelling power than commercial seasoning flour.

  20. Solenostemon monostachyus, Ipomoea involucrata and Carica papaya seed oil versus Glutathione, or Vernonia amygdalina: methanolic extracts of novel plants for the management of sickle cell anemia disease

    National Research Council Canada - National Science Library

    Afolabi, Israel Sunmola; Osikoya, Iyanuoluwa Olubukola; Fajimi, Oluwabukunmi Dorcas; Usoro, Priscilla Ibanga; Ogunleye, Damilola Olufunlayo; Bisi-Adeniyi, Tolulope; Adeyemi, Alaba O; Adekeye, Bosede Temitope

    2012-01-01

    Sickle cell disease (SCD) is a genetic disease caused by an individual inheriting an allele for sickle cell hemoglobin from both parents and is associated with unusually large numbers of immature blood cells, containing many...

  1. Neuroprotective and neurochemical properties of mint extracts

    DEFF Research Database (Denmark)

    López, Víctor; Martín, Sara; Gómez-Serranillos, Maria Pilar

    2010-01-01

    Mints are aromatic plants with a tradition as medicinal remedies and culinary herbs. With the aim of investigating potential central nervous system (CNS) activities of traditional medicinal plants, four species and one hybrid of the genus Mentha (M. aquatica, M. longifolia, M. pulegium, M......(A) receptor). Mentha x piperita and Mentha aquatica produced significant (p

  2. 水生植物床去除富营养化水源水中藻毒素的机制%Mechanism of microcystin removal from eutrophicated source water by aquatic vegetable bed

    Institute of Scientific and Technical Information of China (English)

    宋海亮

    2006-01-01

    为净化富营养化水源水中的藻毒素,在太湖湖滨进行了水生植物床(AVB)技术试验.该技术对MC-RR和MC-LR的平均去除率分别达63.0%和66.7%.用酶联免疫吸附法检测发现水生植物根部对藻毒素的富集能力大于茎叶部位.用荧光原位杂交法在AVB的底泥中检测到2种已知的藻毒素降解细菌,证明微生物降解作用的存在.植物根系原生动物相和后生动物相丰富,盾纤虫、钟虫、旋轮虫、腔轮虫是优势种,原生动物和后生动物的捕食作用在蓝藻和藻毒素的去除中发挥着积极作用.%For purifying raw water for tap water treatment, the aquatic vegetable bed (AVB) experiment has been carried out in a hypertrophic waterfront of Taihu Lake, China. The average removal rates of total microcystin-RR and microcystin-LR are 63.0% and 66. 7%, respectively. Experiments indicate that Ipomoea aquatica can absorb microcystin by using enzyme-linked immunosorbent assay (ELISA), and the roots absorb more toxins than leaves and stems. Fluorescence in situ hybridization (FISH) is used to analyze the density of microcystin degrading bacteria in the AVB sediment. Two species of microcystin degrading bacteria are detected, which indicate that microcystin bio-degradation process happened in the AVB. Protozoa and metazoa are abundant in root spheres. Aspidisca sp., Vorticella sp., Philodina sp., and Lecane sp. are dominant species.The predation functions of protozoa and metazoa have a positive effect on the removal of cyanobacteria and microcystin.

  3. FISH analysis of chromosomes of sweet potato(Ipomoea batatas cv.Xushu No.18)%荧光原位杂交技术分析栽培种甘薯(Ipomoea batatas cv.Xushu No.18)染色体

    Institute of Scientific and Technical Information of China (English)

    汤佳立; 戚大石; 张俞; 刘慧娟; 孙健英; 曹清河; 马代夫; 李宗芸

    2010-01-01

    为了解栽培种甘薯(徐薯18,lpomoea batatas cv.Xushu No.18)的染色体结构,文章利用 45S rDNA荧光原位杂交、自身基因组荧光原位杂交和银染技术对栽培种甘薯进行分子细胞遗传学研究.银染结果显示,徐薯18问期核有6对、8对和9对银染点;45S rDNA荧光原位杂交结果显示,徐薯18染色体上有8对或9对强弱不一的45S rDNA信号;自身基因组荧光原位杂交结果表明,所有染色体的全长分布强烈而密集的杂交信号,着丝粒区、近着丝粒区和端粒区有增强的信号带.

  4. Effect of Combined Plant-Rope Ecological Floating Beds on Improvement of Eutrophic Water Quality%植物-生物绳组合生态浮床对富营养化水体的净化效果

    Institute of Scientific and Technical Information of China (English)

    张亚娟; 刘存歧; 王军霞; 孙磊; 李洪波; 吴亦红

    2012-01-01

    在白洋淀富营养化水体构筑蕹菜(Ipomoea aquatica)浮床-生物绳组合系统一段时间后,带回实验室做静态分析,研究生物绳对微生物富集后对富营养化水体的改善效果.结果表明,蕹菜浮床、蕹菜-聚苯乙烯纤维绳组合浮床、蕹菜,麻绳组合浮床对水中TN、NH4+-N、TP和DIP均有较好的去除效果,蕹菜浮床对水中TN、NH4+-N、TP和DIP的去除率为39.09%、44.55%、63.68%和53.70%,而蕹菜-聚苯乙烯纤维绳组合浮床、蕹菜-麻绳组合浮床的去除率分别为49.46%、67.79%、54.72%、50.62%和60.43%、70.05%、71.70%、64.20%,可见组合式浮床比蕹菜浮床的氮磷去除能力更强,而且以蕹菜-麻绳组合浮床的净化效果最好.其中,蕹菜浮床、蕹菜-聚苯乙烯纤维绳组合浮床和蕹菜—麻绳组合浮床的植物去氮贡献率分别为36.13%、32.15%和27.11%,去磷贡献率分别为71.85%、87.07%和74.34%,表明浮床中植物吸收只去除了系统中一部分N,微生物的脱氮途径占主导作用;而对P的去除,本试验结果显示植物吸收起主要作用.可见,该组合生态浮床是改善富营养化水体的有效方法.%Recently, lake eutrophication has become a serious concern and its causes and controls have attracted growing attention worldwide. In the experiments described here, we have used Ipomoea aquatica as an experimental plant for use with ecological floating beds. Three kinds of combined ecological floating bed systems were constructed by hanging two kinds of rope (hemp and PST) below the beds, and their effectiveness for improving water quality was investigated. The results showed that the plant ecological floating bed, plant-PST- rope combined bed, and plant-hemp-rope combined bed provided significant removal effects on TN, NH/-N, TP and DIP. Removal efficiencies were 39.09% , 49.46% and 60.43% , 44. 55% , 67.79% and 70.05% , 63. 68% , 54. 72% and 71. 70% , and 53. 70% , 50. 62

  5. Dicty_cDB: Contig-U13954-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ate dehydrogenase;... 294 e-111 EU080850_1( EU080850 |pid:none) Phytophthora ipomoeae isolate PD_0... 298 e-...110 EU080843_1( EU080843 |pid:none) Phytophthora ipomoeae isolate PD_0... 298 e-110 AX061868_1( AX061868 |pi...080836 |pid:none) Phytophthora ipomoeae isolate PD_0... 295 e-110 EU080543_1( EU080543 |pid:none) Phytophtho

  6. Cultural Resources Investigations of Larose to Golden Meadow Hurricane Protection Project Levee Section D, Lafourche Parish, Louisiana

    Science.gov (United States)

    1991-03-01

    Hardwoods that comprise the nonwetlands and the hardwood interarea include live oaks (Quercus virginiana), mayhaw ( Crataegus opaca), hackberry (Celtis... laevigata ), bitter pecan (Carya aquatica), pawpaw (Asimina triloba), and sweetgum (Liquidambar styriciflua). Plant understory includes palmetto (Sabal

  7. Cultural Resource Survey of Carrollton Bend Revetment, Mississippi River M-105.7 to 101.7-L, Jefferson and Orleans Parishes, Louisiana

    Science.gov (United States)

    1993-02-26

    and honey locust ( Gleditsia triacanthos ). The understory probably contained shrubs such as buttonbush (Cephalanthus occidentalis), wax myrtle...occidentafis), redgum, black willow (Salix nigra), hackberry (Celtis laevigata), swamp privet (Forestiera acuminata), water locust ( Gleditsia aquatica

  8. Paspalum hydrophylum spec. nov., aus Paraguay

    NARCIS (Netherlands)

    Henrard, J.Th.

    1922-01-01

    Paspalum hydrophylum HENR., spec. nov. — Planta aquatica, perennis, rhizoma crassum. Culmi erecti, metrales vel ultra, simplices, teretiusculi vel subcompressi, laeves, glaberrimi, multinodes, summo nodo supra medium culmi. Vaginae laxae, ecarinatae, leviter striatae, glabrae, internodiis longiores,

  9. Balansseerimine abstraktse liikumise ning tähendusliku loo vahel / Evelin Lagle

    Index Scriptorium Estoniae

    Lagle, Evelin

    2008-01-01

    "Augusti tantsufestival" Tallinnas Kanuti gildi saalis 21.-31. VIII 2008. Tantsulavastustest "Gallina Aquatica", "Creating Sense", "Mi madre y yo", "Ball", "Radiodance", "And I just stood there watching the sky and the people below"

  10. Augustitantsud kanade ja põtradega / Kairi Prints

    Index Scriptorium Estoniae

    Prints, Kairi

    2008-01-01

    21.-23. aug. Tallinna Kanuti Gildi saalis toimunud Augusti tantsufestivali lavastustest - Sonia G̤mezi "Mi madre y yo', Frédéric Giesi "Dance (Practicable), Tatjana Gordejeva "Gallina Aquatica", Iguan Dance Theatre tantsulavastus "Radiodance"

  11. In Vitro Morphogenesis of Arabidopsis to Search for Novel Endophytic Fungi Modulating Plant Growth

    National Research Council Canada - National Science Library

    Dovana, Francesco; Mucciarelli, Marco; Mascarello, Maurizio; Fusconi, Anna

    2015-01-01

    .... In this study, fungi isolated from stems (stem-E) and roots (root-E) of Mentha aquatica L. (water mint) were identified, and their morphogenetic properties analysed on in vitro cultured Arabidopsis...

  12. Augustitantsud kanade ja põtradega / Kairi Prints

    Index Scriptorium Estoniae

    Prints, Kairi

    2008-01-01

    21.-23. aug. Tallinna Kanuti Gildi saalis toimunud Augusti tantsufestivali lavastustest - Sonia G̤mezi "Mi madre y yo', Frédéric Giesi "Dance (Practicable), Tatjana Gordejeva "Gallina Aquatica", Iguan Dance Theatre tantsulavastus "Radiodance"

  13. Balansseerimine abstraktse liikumise ning tähendusliku loo vahel / Evelin Lagle

    Index Scriptorium Estoniae

    Lagle, Evelin

    2008-01-01

    "Augusti tantsufestival" Tallinnas Kanuti gildi saalis 21.-31. VIII 2008. Tantsulavastustest "Gallina Aquatica", "Creating Sense", "Mi madre y yo", "Ball", "Radiodance", "And I just stood there watching the sky and the people below"

  14. Evaluation of Iodine-131 dispersion after accident in Nuclear Angra Power Plant using the model of aquatic dispersion, SisBahia; Avaliacao da dispersao de iodo-131 apos um acidente na usina de Angra utilizando o modelo de dispersao aquatica, SisBahia

    Energy Technology Data Exchange (ETDEWEB)

    Aguiar, Andre Silva de; Alvim, Antonio Carlos Marques, E-mail: aguiargm@gmail.com, E-mail: aalvim@gmail.com [Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ (Brazil). Centro de Tecnologia; Simoes Filho, Francisco Fernando Lamego, E-mail: flamego@ien.gov.br [Instituto de Engenharia Nuclear (IEN/CNEN-RJ), Rio de Janeiro, RJ (Brazil)

    2014-07-01

    It was inserted, in the cooling system of the nucleus, a LOCA, where 431 m{sup 3} of coolant was lost. Such an inventory contained 3,04 x 10{sup 10} Bq / m{sup 3} and iodine was released near the beach Itaorna, Angra dos Reis - RJ. Applying the model in the proposed scenario (Angra 1 and Angra 2 in operation and 3 in with varying capture and discharge with the discharge gradually reduced after the accident), the dilution of the specific activity of radionuclides reached lower values after 22 hours, the reference levels for seawater {sup 131}I (7,40 x 10{sup 2} Bq / m{sup 3}). After 54 hours, levels of the radionuclide, in the area of indirect influence, are already below minimum activity values detected by the laboratory for environmental monitoring of the CNAAA (5,0 x 10{sup 1} Bq / m{sup 3}.

  15. Inter-annual variation (1991-1993) of the substratum-leaf colonization dynamics for aquatic fauna in different habitats of the lake of the hydroelectric of Balbina, Amazon Central, Brazil; Variacao interanual (1991-1993) da dinamica de colonizacao de substrato-folha por fauna aquatica, em diferentes habitats do lago da Hidreletrica de Balbina, Amazonia Central, Amazonas- Brasil

    Energy Technology Data Exchange (ETDEWEB)

    Vela-Pena, Gladys

    1996-07-01

    Experiments on fauna colonization of submersed vegetal substrate in different depths of water column were done to evaluate the benthic community structure in three habitats of the Balbina hydroelectric dam in 1991, 1992 and 1993. In these experiments substrate exposition periods of up to 60 and 75 days were done. The fauna associated to the standard substrate (Mabea caudata) belonged to seven phyla: Arthropoda, Coelenterata, Nematoda, Bryozoa, Annelida, Mollusca an Chordata. The most abundant and frequent families, during the studied period, were Naididae (Tubificida), Chydoridae (Cladocera) and Cenestheridae (Conchostraca), suggesting the persistence of these groups. In general, the pattern of colonization indicates some tendency to increase gradually with time of exposition of the substrate in the environment. Probably, the discontinuity of the tendencies is associated with the insects mobility and emergence. The initial colonization always was higher and quicker in the margin habitat, which indicates that the source of organisms is this habitat. This is due to better conditions of the environment such as availability of food and protection, associated with the submerged vegetation and wood. The community mean density during this study was 7, 312 ind/m{sup 2}. The density, the species richness index, and the diversity were correlated with abiotic variables such as pH, temperature, dissolved oxygen, habitat and depth. Also, the density was correlated with total carbon and ammonium. Species richness was correlated with total carbon, ammonium and water color. The density, diversity and species richness were proportionally inverse to depth of the habitats and total absence of organisms ago 10 meter of depth, different from what is found in bottom of natural environments. This fact was attributed to the high concentration of nutrients, such as ammonium and dissolved iron, to the existence of toxic gases such a sulphide, and to the conditions of hypoxia in the deep layers. The stability of relative abundance in some groups and the persistence of dominant taxons can be seen as characteristics of the lentic environment. (author)

  16. Uptake of /sup 131/I by some hydroponically grown crops

    Energy Technology Data Exchange (ETDEWEB)

    Asprer, G.A.; Lansangan, L.M.; de la Paz, L.R. (Philippine Atomic Energy Commission, Diliman, Quezon City)

    Biologically labelled vegetables which include kangkong and sweet potato tops were grown hydroponically in a modified Hoagland-Arnon nutrient solution containing radioiodine with 0.5% non-radioactive Nal solution as the medium. The crops considered in this study are commonly eaten by Filipinos. The concentration of the solution as well as the uptake in the plant system were determined at various time intervals. The extent of radioiodine uptake through air-water-plant pathway is one of the parameters needed for calculating the dose that the general populace could be exposed to, due to radioactivity in the environment.

  17. 2种复合肥对甘薯产量性状的影响%Effect of Different Compound Fertilizer on Yield of Sweet Potato(Ipomoea batatas L.Lam)

    Institute of Scientific and Technical Information of China (English)

    李观康; 陈胜勇; 何霭如; 余小丽; 汪云

    2014-01-01

    Different fertilizer levels of Weifang Potassium Sulphate Fertilizer and Finland Fertilizer were applied in the filed.The root fresh yield,starch content,dry matter content,vine yield and other indexes in sweet potato were determined in order to find out the effect of compound fertilizer on yield of sweet potato and the compound fertilizer utilization regular.The results showed that the fertilizer amount that the highest yield and the best benefit could be obtained in sweet potato production was applying Weifang Potassium Sulphate Fertilizer 375 kg/hm2.Weifang Potassium Sulphate Fertilizer was a new compound fertilizer and the special fertilizer for sweet potato.%以潍坊硫酸钾复合肥和芬兰复合肥的不同施肥量为处理,测定甘薯鲜薯产量、淀粉含量、干率、茎叶产量和其他主要指标,综合评价不同复合肥对甘薯产量性状的影响,找出当地甘薯生产复合肥料的利用规律,减少甘薯生产盲目施肥。结果表明:甘薯生产中最高产和最佳效益施肥量均为潍坊硫酸钾复合肥375 kg/hm2,潍坊硫酸钾复合肥这种新型复合肥可作为甘薯专用肥施用。

  18. KARAKTERISTIK FISIKO-KIMIA TEPUNG UBI JALAR (Ipomoea batatas VARIETAS SUKUH DENGAN VARIASI PROSES PENEPUNGAN [Physico-Chemical Characteristics of Sukuh Variety Sweet Potatoes (Ipomea batatas Flours Made with Various Methods

    Directory of Open Access Journals (Sweden)

    Trifena Honestin

    2009-12-01

    Full Text Available Sweet potato has considerable potencies to support food diversification program based on flour and starch product. Various processing methods in the flour processing show great effect on alteration of the physicochemical properties of sweet potato flour. This research investigated the effects of different flour processing methods on the physicochemical properties of sweet potato flour. The results showed that processing method had a significant effect on water content, bulk density, colour (L, a, b, microscopic properties of starch granule, water absorption index, water soluble index and pasting properties of the sweet potato flour.

  19. KARAKTERISASI TEPUNG UBI JALAR (Ipomoea batata L. VARIETAS SHIROYUTAKA SERTA KAJIAN POTENSI PENGGUNAANNYA SEBAGAI SUMBER PANGAN KARBOHIDRAT ALTERNATIF [Characterization of Sweet Potato Flour (Ipomea batatas L. var. Shiroyutaka and Assesment of the potential as Alternative Carbohydrate Source For Food Product

    Directory of Open Access Journals (Sweden)

    Beni Hidayat 1

    2007-06-01

    Full Text Available This research was aimed to characterize sweet potato flour var. shiroyutaka and assessment of its potential as alternative carbohydrate source on food product. Characterization was conducted on flour processed from sweet potato var. shiroyutaka harvested at four months was characteristic, these included whiteness degree and water absorption of the flour, ratio of amylase-amylopectin, form and size of starch granule, and starch digestibility.The research showed that whiteness degree and water absorption of the flour were 78,82% ( 0,52 and 1,25 g/g ( 0,12 respectively. The ratio of amylase-amylopectin, gelatinization temperature, maximum viscosity and invitro starch digest ability were 69.82%: 30.18%, 78-900C and 84,78% respectively. The granule of its starch was round form and with size 2-4 micron.The main potential of the flour is related with its specific characteristics which were the amylose-amylopectin ratio, the starch amylograph profile, form and size of starch granule, and the starch digest ability. These parameters implied that, the flour should be utilized in the production specific food products.

  20. Composición mineral y comparación de raíces reservantes de variedades de batatas (Ipomoea batatas (L. Lam. cultivadas en la isla de La Palma, España

    Directory of Open Access Journals (Sweden)

    Adriana Isabel Montes Hernández

    2010-07-01

    Full Text Available Los minerales Na, K, Ca, Mg, Fe, Cu, Zn y Mn fueron determinados, mediante espectrofotometría de absorción atómica, en raíces de 15 variedades de batatas cultivadas en La Palma (De papa o maní, Parado, Cubana, Cubana de baselo gordo, Cubana de hoja redonda, Blanquita, Saucero o Lanzarotero, Pata de Gallo, Padrón de Seda, Alicantina, Amarillo de Año, De Lanzarote, Matojo, Matojo Fino y Rajada; las 5 primeras de cultivo corto y las 10 restantes de cultivo largo. Se realizó análisis de varianza y prueba de rango múltiple de Duncan a los datos de las variables estudiadas. Se calcularon los coeficientes de correlación de Pearson (r para establecer grados de relación de los minerales entre las variedades; prueba de t de Student para comparar clasificando a las muestras según ciclo de recolección (Temprana y Tardía; análisis factorial para interpretar las relaciones existentes a través factores y análisis discriminantes (de paso a paso con criterio de selección la lambda de Wilks (λ e introduciendo todas las variables validados a través de análisis de validación cruzada. El elemento mineral (en mg/kg presente en mayor concentración en la totalidad de las muestras fue K (2086 ± 418, seguido de Ca (974 ± 486 y Mg (244 ± 70, siendo el Zn el menos presente (1,12 ± 0,61. Las variedades con mayor contenido mineral fueron Alicantina (Ca: 1809 ± 327 y Cu: 2,97 ± 0,23 y Padrón de Seda (Mg: 435 ± 46 y Mn: 3,08 ± 0,49 y la de menor aporte fue Parado (Cu, Mn, Ca, K y Mg. Las variedades Tardías presentaron mayores concentraciones de Cu, Fe, Mn, Ca, K, Mg y menores de Zn y Na en relación a las variedades Tempranas (p < 0,05. Se evidenció que las concentraciones de Cu, Zn y Na presentaron alto número de correlaciones significativas. El análisis factorial permitió deducir que Mg, K y Fe son las variables que permiten caracterizar el sistema perdiendo un mínimo de información total. El porcentaje de casos originales correctamente clasificados después de aplicar análisis discriminantes a las variedades de ciclo de recolección Temprana fue del 98 % de los casos originales y 96 % luego de la validación cruzada, mientras que para las variedades de ciclo de recolección Tardía fue del 100 % de los casos originales y 99 % después de la validación.

  1. Study on Antioxidant Activity of Extracts from Ipomea Pes-caprae (Linn.) Sweet%海滩植物厚藤(Ipomoea Pes-caprae)抗氧化活性研究

    Institute of Scientific and Technical Information of China (English)

    刘平怀; 陈德力; 汪春牛; 许琼情; 刘洋洋

    2010-01-01

    采用加速溶剂萃取技术(ASE)快速制备厚藤活性提取物,并以DPPH法对厚藤茎叶不同溶剂提取物抗氧化活性进行评价.抗坏血酸抗氧化能力>乙酸乙酯提取物抗氧化能力>乙醇提取物抗氧化能力>甲醇提取物抗氧化能力>石油醚提取物抗氧化能力,其IC50值分别为0.203 4、0.836 2、0.868 8、1.867 5、6.628 3 g/L.结果显示,厚藤茎叶提取物具有一定的抗氧化活性,且以乙酸乙酯为提取溶剂时,效果最佳.

  2. Influência da adição da fécula de batata-doce (Ipomoea batatas L. sobre a viscosidade do permeado de soro de queijo Influence of sweet potato starch at permeate whey viscosity

    Directory of Open Access Journals (Sweden)

    Rafael Leite Pinto de Andrade

    2002-12-01

    Full Text Available A viscosidade é um parâmetro importante na aceitabilidade de bebidas lácteas. O presente trabalho teve como objetivo principal fornecer dados relativos ao comportamento da viscosidade uma base de permeado de soro de queijo adicionada de fécula de batata-doce, dados que poderão ser utilizados na elaboração de uma nova bebida láctea. A fécula de batata-doce utilizada apresentou um grau de gelatinização de 72,64%, umidade média de 7,88% e a seguinte composição centesimal média em base seca: 82,59% de amido, 9,33% de fibras dietéticas totais, 4,90% de proteínas, 2,08% de cinzas e 1,11% de lipídios. O permeado de soro foi adicionado a três diferentes concentrações de fécula e submetido a diferentes tratamentos térmicos. A viscosidade mais adequada, em função dos dados obtidos junto aos produtos comerciais, estaria no intervalo de 45mPa.s a 70mPa.s. Assim sendo, a porcentagem de 6% de fécula de batata-doce e um tratamento térmico de 90ºC por cerca de 5 minutos mostrou-se ser o mais adequado. O fluido obtido apresentou um comportamento pseudoplástico.Viscosity is an important parameter of milky drinks acceptability. This work supply data of the viscosity properties of permeate whey plus sweet potato starch basis; these data will may be used at development of a new milky beverage. The used sweet potato starch presented 72.64% of gelatinization, 7.88% of humidity and the following dry composition: 82.59% starch, 9.33% total dietary fibres, 4.90% proteins, 2.08% ashes and 1.10% lipids. The permeate whey had mixed at three different percentages of potato starch and had submitted at various thermal treatments. The most appropriate viscosity, in accord of commercial products, it would be in the interval of 45mPa.s to 70mPa.s. Therefore, the percentage of 6% of sweet potato starch and a thermal treatment of 90ºC for about 5 minutes it was shown to be the most appropriate. The fluid presented a pseudoplastic properties.

  3. Transcriptional profiling of sweetpotato (Ipomoea batatas) roots indicates down-regulation of lignin biosynthesis and up-regulation of starch biosynthesis at an early stage of storage root formation

    Science.gov (United States)

    2013-01-01

    Background The number of fibrous roots that develop into storage roots determines sweetpotato yield. The aim of the present study was to identify the molecular mechanisms involved in the initiation of storage root formation, by performing a detailed transcriptomic analysis of initiating storage roots using next-generation sequencing platforms. A two-step approach was undertaken: (1) generating a database for the sweetpotato root transcriptome using 454-Roche sequencing of a cDNA library created from pooled samples of two root types: fibrous and initiating storage roots; (2) comparing the expression profiles of initiating storage roots and fibrous roots, using the Illumina Genome Analyzer to sequence cDNA libraries of the two root types and map the data onto the root transcriptome database. Results Use of the 454-Roche platform generated a total of 524,607 reads, 85.6% of which were clustered into 55,296 contigs that matched 40,278 known genes. The reads, generated by the Illumina Genome Analyzer, were found to map to 31,284 contigs out of the 55,296 contigs serving as the database. A total of 8,353 contigs were found to exhibit differential expression between the two root types (at least 2.5-fold change). The Illumina-based differential expression results were validated for nine putative genes using quantitative real-time PCR. The differential expression profiles indicated down-regulation of classical root functions, such as transport, as well as down-regulation of lignin biosynthesis in initiating storage roots, and up-regulation of carbohydrate metabolism and starch biosynthesis. In addition, data indicated delicate control of regulators of meristematic tissue identity and maintenance, associated with the initiation of storage root formation. Conclusions This study adds a valuable resource of sweetpotato root transcript sequences to available data, facilitating the identification of genes of interest. This resource enabled us to identify genes that are involved in the earliest stage of storage root formation, highlighting the reduction in carbon flow toward phenylpropanoid biosynthesis and its delivery into carbohydrate metabolism and starch biosynthesis, as major events involved in storage root initiation. The novel transcripts related to storage root initiation identified in this study provide a starting point for further investigation into the molecular mechanisms underlying this process. PMID:23834507

  4. Inheritance Analysis and SCAR Marker of the Gene for Stem Nematode Resistance, in Sweetpotato, Ipomoea batatas (L.) Lam.%甘薯抗茎线虫病基因的遗传分析及SCAR标记

    Institute of Scientific and Technical Information of China (English)

    揭琴; 姜伟; 李华; 翟红; 马代夫; 谢逸萍; 刘庆昌

    2008-01-01

    以甘薯抗茎线虫病品种徐781和感茎线虫病品种徐薯18的杂交F1分离群体的174株单株为材料,对甘薯抗茎线虫病基因的遗传进行了分析.结果表明,徐781的抗茎线虫病为单基因控制,推测其基因型为Rrrrrr,徐薯18的基因型为rrrrr.采用BSA-RAPD相结合的方法,筛选940条随机引物,发现10条引物在抗、感池间表现多态性.用这10条引物检测两亲本及建池单株,发现只有引物OPP03在8株抗池单株中扩增出一条在8株感池单株中所没有的特异条带,认为该标记与甘薯抗茎线虫病基因连锁.根据该标记对F1代174个单株的扩增结果,利用Mapmaker 3.0软件计算遗传距离,表明该标记与抗茎线虫病基因间的遗传距离为14.2 cM.将该片断回收、克隆、测序,表明其长度为878 bp,该标记命名为OPP03878.根据测序结果,设计1对特异引物,进行特异性扩增,成功地将OPP03878标记转化为SCAR标记,用亲本及分离群体验证表明该分子标记稳定性好.

  5. Composición mineral y comparación de raíces reservantes de variedades de batatas (Ipomoea batatas (L. Lam. cultivadas en la isla de La Palma, España

    Directory of Open Access Journals (Sweden)

    Adriana Isabel Montes Hernández

    2010-06-01

    Full Text Available Los minerales Na, K, Ca, Mg, Fe, Cu, Zn y Mn fueron determinados, mediante espectrofotometría de absorción atómica, en raíces de 15 variedades de batatas cultivadas en La Palma (De papa o maní, Parado, Cubana, Cubana de baselo gordo, Cubana de hoja redonda, Blanquita, Saucero o Lanzarotero, Pata de Gallo, Padrón de Seda, Alicantina, Amarillo de Año, De Lanzarote, Matojo, Matojo Fino y Rajada; las 5 primeras de cultivo corto y las 10 restantes de cultivo largo. Se realizó análisis de varianza y prueba de rango múltiple de Duncan a los datos de las variables estudiadas. Se calcularon los coeficientes de correlación de Pearson (r para establecer grados de relación de los minerales entre las variedades; prueba de t de Student para comparar clasificando a las muestras según ciclo de recolección (Temprana y Tardía; análisis factorial para interpretar las relaciones existentes a través factores y análisis discriminantes (de paso a paso con criterio de selección la lambda de Wilks (λ e introduciendo todas las variables validados a través de análisis de validación cruzada. El elemento mineral (en mg/kg presente en mayor concentración en la totalidad de las muestras fue K (2086 ± 418, seguido de Ca (974 ± 486 y Mg (244 ± 70, siendo el Zn el menos presente (1,12 ± 0,61. Las variedades con mayor contenido mineral fueron Alicantina (Ca: 1809 ± 327 y Cu: 2,97 ± 0,23 y Padrón de Seda (Mg: 435 ± 46 y Mn: 3,08 ± 0,49 y la de menor aporte fue Parado (Cu, Mn, Ca, K y Mg. Las variedades Tardías presentaron mayores concentraciones de Cu, Fe, Mn, Ca, K, Mg y menores de Zn y Na en relación a las variedades Tempranas (p < 0,05. Se evidenció que las concentraciones de Cu, Zn y Na presentaron alto número de correlaciones significativas. El análisis factorial permitió deducir que Mg, K y Fe son las variables que permiten caracterizar el sistema perdiendo un mínimo de información total. El porcentaje de casos originales correctamente clasificados después de aplicar análisis discriminantes a las variedades de ciclo de recolección Temprana fue del 98 % de los casos originales y 96 % luego de la validación cruzada, mientras que para las variedades de ciclo de recolección Tardía fue del 100 % de los casos originales y 99 % después de la validación.

  6. Study on antitumor effect and its toxicity of Ipomoea Batatas Poir Cv anthocyanins%青紫薯色素抗肿瘤作用及毒理学实验研究

    Institute of Scientific and Technical Information of China (English)

    刘宁; 王红兵; 王春波

    2008-01-01

    目的 研究青紫薯色素抗肿瘤作用并对其毒性进行安全性评价.方法 以小鼠肉瘤S180株和小鼠肝癌H22,瘤株为实验对象,每日分别灌胃给予阳性药呋喃氟尿嘧啶及150、75和37.5mg的青紫薯色素,通过计算肿瘤抑制率观察青紫薯色素的体内抗肿瘤作用;通过亚慢性毒性试验以及骨髓微核试验、Ames试验对青紫薯色素进行毒理学评价.结果 青紫薯色素灌胃,每日150、75mg剂量对小鼠肉瘤S180的抑瘤率分别为45.04%和36.64%.37.5mg剂量无抑瘤作用;青紫薯色素每日150mg剂量对小鼠肝癌H22有明显抑制作用,抑瘤率达33.33%;青紫薯色素1000met(kg·d)灌胃对大鼠未见明显毒性反应;在骨髓微核试验、Ames试验中均呈阴性结果.结论 青紫薯色素对小鼠移植瘤有抑制作用,基本无毒性,无致突变作用.

  7. Identificación de dos spp de Ipomoea y evaluación in vitro del extracto metanólico como problema de intoxicación en cabras.

    OpenAIRE

    Mila Arango, Roberta

    2013-01-01

    El desconocimiento de distintas plantas tóxicas y la ingesta de éstas por rebaños caprinos en temporada de sequía, ha provocado que muchos casos de intoxicación no se diagnostiquen o sean confundidos con otras causas, provocando altos índices de morbilidad y mortalidad en rebaños caprinos. El presente estudio tuvo como objetivo principal, la identificación de los metabolitos secudarios presentes en dos especies arbustivas de la Mixteca baja de Oaxaca causantes de intoxicación en cabras. Se ev...

  8. Phylogenetics and diversification of morning glories (tribe ipomoeeae, convolvulaceae) based on whole plastome sequences

    Science.gov (United States)

    Phylogenetic studies have demonstrated the largest morning glory genus, Ipomoea, is not monophyletic, and nine other segregate genera are derived from within Ipomoea. Therefore, systematic research is focused on the monophyletic tribe Ipomoeeae (c. 650-900 species). We used whole plastid genomes to ...

  9. Dicty_cDB: CHR467 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available enomic 5', sequence tagged site. 174 1e-67 2 CJ769526 |CJ769526.1 Ipomoea nil cDNA clone:jmsf9f07, 3' end. 4...2 9.3 1 CJ753155 |CJ753155.1 Ipomoea nil cDNA clone:jmsf9f07, 5' end. 42 9.3 1 dn

  10. Effects of Simulated Acid Rain on the Migration and Transformation of Hexavalent Chromium in a Water Spinach-soil System%模拟酸雨对空心菜-土壤系统中六价铬迁移转化的影响

    Institute of Scientific and Technical Information of China (English)

    陈桂葵; 李志鹏; 陈恋; 刘明中; 黎华寿

    2013-01-01

    A pot experiment was conducted to study the effects of acid rain on the distribution, migration and transformation of hexavalent chromium in a water spinach(Ipomoea aquatica) soil system. Results showed that the amount of total chromium in water spinach treated with simulated acid rain was significantly higher than those without acid rain. The content of chromium in the underground part of water spinach was significantly higher than it in the aboveground part in treatments with simulated acid rain. While water spinach could survive and grow normally in soil with 100 mg·kg-1 chromium, they couldn't survive in soil with chromium up to 200 mg·kg-1. Valences of more than 97.0% added hexavalent chromium was changed. The majority portion of the chromium was remained in soil. Only very small portion was absorbed by water spinach. The simulated acid rain could significantly enhance the absorption rate of chromium by water spinach. The amount of absorbed chromium in treatments with acid rain was 2.6 to 5 times as those in the treatments without acid rain. The chromium enrichment coefficients were 2.2 and 4.6 for the aboveground part and the underground part of water spinach respectively by comparing the treatments with and without acid rain. The research result shows that acid rain increases the risk of crop grown in soil contaminated by chromium especially through the absorption of chromium by root system. Hence, agricultural food safety problem in chromium polluted area will be enhanced by acid rain.%通过盆栽试验,研究了模拟酸雨条件下六价铬在土壤和空心菜之间的分布、迁移和转化规律.结果发现,酸雨处理显著提高了空心菜体内铬的含量,其中空心菜地下部的铬含量显著高于其地上部;空心菜在100 mg·kg-1的铬处理下仍然可以正常生长,但当土壤中的铬达到200 mg· kg-1时,空心菜生长数天后即全部死亡;添加到土壤中的六价铬97.0%以上已经转化成其他形态而留

  11. Protection of food crops during rapid development of the Palinpinon Geothermal Project

    Energy Technology Data Exchange (ETDEWEB)

    Darby, d' E.C. (KRTA Ltd., Auckland, New Zealand); de Jesus, A.C.

    1981-10-01

    A tropical water plant known as kangkong is cultivated in the Okoy River. Many hundreds of people are involved in growing this important green vegetable which is harvested up to 12 times per year, hence the need to avert major damage to crops is clear. Trials suggest that kangkong is sensitive to lower levels of arsenic than boron, but because of the relative amounts of these elements in geothermal waters boron is likely to be the limiting element in regard to surface waste-water discharges. Arsenic or boron toxicity symptoms were more severe in the presence of sulphate, while high calcium levels delayed the onset or reduced the severity of the symptoms. Plants tolerated thermal shocks up to about 50/sup 0/C for 30 minutes. Under the test conditions the maximum continuously tolerable level of geothermal fluid was about 8% and of As and B about 3 mg/kg and 5 mg/kg, respectively. For purposes of crop protection during project development, however, wastewater discharges from wells under test are normally regulated so that the level of B upstream of the cropping area does not normally exceed about 3 mg/kg.

  12. Variation in mating systems of salamanders: mate guarding or territoriality?

    Science.gov (United States)

    Deitloff, Jennifer; Alcorn, Michael A; Graham, Sean P

    2014-07-01

    Two of the most common mating tactics in vertebrates are mate guarding and territoriality, yet much of the research on these strategies has focused on mating systems in birds, despite novel insights gained from studying less traditional systems. North American stream salamanders that comprise the Eurycea bislineata complex represent an excellent nontraditional system for comparing mating strategies because these species exhibit a continuum of male morphologies, diverse habitat associations, and various potential mating strategies. We studied two species within this complex that exhibit the extremes of this continuum, Eurycea aquatica (robust morph) and Eurycea cirrigera (slender morph). The larger head in males of E. aquatica is due to larger musculature around the jaw and may be associated with aggressive behavior. Therefore, we hypothesized that the robust morphology exhibited by males of E. aquatica provides benefits during either territorial defense or mate defense and that males of E. cirrigera would not exhibit aggression in either scenario. We found that neither species exhibited aggressive behavior to defend a territory. However, in the presence of a female, males of E. aquatica were significantly more aggressive toward intruding males than were males of E. cirrigera. Therefore, mate-guarding behavior occurs in E. aquatica, and the enlarged head of males likely aids in deterring rivals. This is the first demonstration of mate-guarding behavior in a plethodontid, the most speciose family of salamanders.

  13. 电子垃圾拆解区土壤上空心菜的毒理响应%Toxicology Studies on Soil of Electronic Waste Recycling Site to Spinach

    Institute of Scientific and Technical Information of China (English)

    王瑾丰; 宋小飞; 牛晓君; 吴浩; 于敏; 李丽; 刘尘

    2014-01-01

    soil was collected with a distance of 2, 20, 40 and 50 m separately from e-waste dump sites and the uncontaminated soil was also collected for comparing. The toxicological Cd, Cu, Pb, Zn, and Cr response of Ipomoea aquatica was conducted in the laboratory with plastic basin. The results indicated that five heavy metals distribution in water spinach planted on e-waste contaminated soil was root> stem> leaf and the highest enrichment factor was belonged to root which also acuminate Cu and Zn at a higher level. Furthermore, the paddy soil contributed to a higher bio-concentration; under the stress of the heavy metal contamination, the leaf form changed dramatically while the height among the plant was not significant, and photosynthetic capacity of spinach decreased obviously at the same time: the content of chlorophyll in the plant planted on the soil of 2, 20, 40 and 50 m from the e-waste dump sites were 44.0%, 74.2%, 75.4%, 88.7%, and 74.1%, separately, to the control group’s; the plant could well adjusted to the heavy metal polluted soil and the parameter of catalase and peroxidase activities were similar: the activity of catalase and peroxidase increased with the higher level of soil polluted by e-waste.

  14. Hydrolytic fragmentation of seed gums under microwave irradiation.

    Science.gov (United States)

    Singh, V; Tiwari, A

    2009-03-01

    The seed gum solutions of Ipomoea purga, Ipomoea palmata, Ipomoea dasysperma, Cyanaposis tetragonolobus (Guar gum) and Crotolaria medicaginea were microwave (MW) irradiated and their degradation to oligo and monosaccharides was investigated. The gum solutions were fragmented into oligosaccharides/constituent monosaccharides depending upon the length of MW exposure in presence of catalytic amount of mineral acid or even when no acid was used. A mechanism for the microwave induced hydrolytic degradation of the seed gums has been proposed. The MW exposure time required for the partial and complete degradation of the gums was found dependent on the types of the linkages and degree of the branching present in the gums.

  15. Participatory consumer evaluation of twelve sweetpotato varieties in ...

    African Journals Online (AJOL)

    STORAGESEVER

    2010-03-15

    Mar 15, 2010 ... Key words: Sweetpotato, participatory, ipomoea batatas, consumer, preference, varieties Kenya. .... show any symptom of water stress after missing rains for one ..... (2004) reported that in rural communities in Africa are.

  16. Volume 10 No. 11 November 2010 4413 RETENTION OF ...

    African Journals Online (AJOL)

    user

    2010-11-11

    Nov 11, 2010 ... antioxidant activity (AOA) of Tainong 66 cultivar sweet potato (Ipomoea batatas. Lam.) ... Health promotion and disease prevention by appropriate diets have drawn the ..... Concentration in Processed sweet potato Chips.

  17. Genetic diversity of sweet potatoes collection from Northeastern Brazil

    African Journals Online (AJOL)

    Ana Veruska Cruz da Silva Muniz

    2014-02-24

    Feb 24, 2014 ... The sweet potato, Ipomoea batatas (L.) Lam has its origin in Tropical America. ... breeders to compare the phenotypic effects of genes or ... developmental and environmental variations. ..... Crop Evolution 45:271-277.

  18. effect of fluazitopbutyl and atrazine/metolachlor for weed control in ...

    African Journals Online (AJOL)

    COCOYAM

    Yields were collected by 50kg weighing balance. ... Sweetpotato (Ipomoea batatas (L) Lam) is an annual perennial herbaceous crop ... Survey reports in Nigeria show that production, .... New development in processing sweetpotato for food.

  19. Sweetpotato breeding for northeastern Uganda: farmer varieties, farmer-participatory selection, and stability of performance

    NARCIS (Netherlands)

    Abidin, P.E.

    2004-01-01

    Keywords: Agro-biodiversity, farmer varieties, indigenous knowledge, farmer-participatory research, genetic diversity, genotype-by-environment interaction, germplasm collection, Ipomoea batatas , specific adaptation, yield stability, sweetpotato, variance component estimates.

  20. Global Journal of Pure and Applied Sciences - Vol 16, No 2 (2010)

    African Journals Online (AJOL)

    Performance of maize (Zea mays l.) ... Growth and yield responses of sweet potato (ipomoea batatas) to time of potassium fertilzer ... Effect of gasket of varying thickness on spark ignition engines · EMAIL FULL TEXT EMAIL FULL TEXT