Sample records for k5 kd5 k45

  1. Optimizing Your K-5 Engineering Design Challenge (United States)

    Coppola, Matthew Perkins; Merz, Alice H.


    Today, elementary school teachers continue to revisit old lessons and seek out new ones, especially in engineering. Optimization is the process by which an existing product or procedure is revised and refined. Drawn from the authors' experiences working directly with students in grades K-5 and their teachers and preservice teachers, the…

  2. Bringing Inquiry Science to K-5 Classrooms (United States)

    Schachtel, Paula L.; Messina, D. L.; McDermott, L. C.


    As a science coach in the Seattle School District, I am responsible for helping other elementary teachers teach science. For several years, I have been participating in a program that consists of intensive NSF Summer Institutes and an ongoing academic-year Continuation Course. Teachers in this program work through modules in Physics by Inquiry, a research-based curriculum developed by the Physics Education Group at the University of Washington.1 I will discuss how this type of professional development has deepened my understanding of topics in physical science, helped me to teach science by inquiry to my own students, and enabled me to assist my colleagues in implementing inquiry science in their K-5 classrooms. Sponsored by Lillian C. McDermott. 1. A research-based curriculum developed by L.C. McDermott and the Physics Education Group at the University of Washington, Physics by Inquiry, New York, NY, John Wiley & Sons, Inc. (1996.)

  3. Math and Economics: Implementing Authentic Instruction in Grades K-5 (United States)

    Althauser, Krista; Harter, Cynthia


    The purpose of this study is to outline a partnership program that involved a local elementary school district, an institution of higher education, the local business community, and a state economic education advocacy group to integrate economics into math in grades K-5. The "Economics: Math in Real Life" program was provided in…

  4. Spectroscopic properties of K5Li2UF10

    International Nuclear Information System (INIS)

    Karbowiak, M.; Gajek, Z.; Drozdzynski, J.


    A new uranium (III) fluoro-complex of the formula K 5 Li 2 UF 10 has been synthesised and characterised by X-ray powder diffraction and electronic absorption spectra measurements. The compound crystallises in the orthorhombic system, space group Pnma, with a = 20.723, b = 7.809, c = 6.932 A, V = 1121.89 A 3 , Z = 4 and is isostructural with its K 5 Li 2 NdF 10 and K 5 Li 2 LaF 10 analogous. The absorption spectrum of a polycrystalline sample of K 5 Li 2 UF 10 was recorded at 4.2 K in the 3500-45,000 cm -1 range and is discussed. The observed crystal-field levels were assigned and fitted to parameters of the simplified angular overlap model (AOM) and next to those of a semi-empirical Hamiltonian, which was representing the combined atomic and one-electron crystal-field interactions. The starting values of the AOM parameters were obtained from ab initio calculations. The analysis of the spectra enabled the assignment of 71 crystal-field levels of U 3+ with a relatively small r.m.s. deviation of 37 cm -1 . The total splitting of 714 cm -1 was calculated for the 4 I 9/2 ground multiplet

  5. Spectroscopic properties of K 5Li 2UF 10 (United States)

    Karbowiak, M.; Gajek, Z.; Drożdżyński, J.


    A new uranium (III) fluoro-complex of the formula K 5Li 2UF 10 has been synthesised and characterised by X-ray powder diffraction and electronic absorption spectra measurements. The compound crystallises in the orthorhombic system, space group Pnma, with a = 20.723, b = 7.809, c = 6.932 Å, V = 1121.89 Å 3, Z = 4 and is isostructural with its K 5Li 2NdF 10 and K 5Li 2LaF 10 analogous. The absorption spectrum of a polycrystalline sample of K 5Li 2UF 10 was recorded at 4.2 K in the 3500-45,000 cm -1 range and is discussed. The observed crystal-field levels were assigned and fitted to parameters of the simplified angular overlap model (AOM) and next to those of a semi-empirical Hamiltonian, which was representing the combined atomic and one-electron crystal-field interactions. The starting values of the AOM parameters were obtained from ab initio calculations. The analysis of the spectra enabled the assignment of 71 crystal-field levels of U 3+ with a relatively small r.m.s. deviation of 37 cm -1. The total splitting of 714 cm -1 was calculated for the 4I 9/2 ground multiplet.

  6. Increased suppression of oncolytic adenovirus carrying mutant k5 on colorectal tumor

    International Nuclear Information System (INIS)

    Fan Junkai; Xiao Tian; Gu Jinfa; Wei Na; He Lingfeng; Ding Miao; Liu Xinyuan


    Angiogenesis plays a key role in the development of a wide variety of malignant tumors. The approach of targeting antiangiogenesis has become an important field of cancer gene therapy. In this study, the antiangiogenesis protein K5 (the kringle 5 of human plasminogen) has been mutated by changing leucine71 to arginine to form mK5. Then the ZD55-mK5, which is an oncolytic adenovirus expressing mK5, was constructed. It showed stronger inhibition on proliferation of human umbilical vein endothelial cell. Moreover, in tube formation and embryonic chorioallantoic membrane assay, ZD55-mK5 exhibited more effective antiangiogenesis than ZD55-K5. In addition, ZD55-mK5 generated obvious suppression on the growth of colorectal tumor xenografts and prolonged the life span of nude mice. These results indicate that ZD55-mK5 is a potent agent for inhibiting the tumor angiogenesis and tumor growth

  7. 26 CFR 1.401(k)-5 - Special rules for mergers, acquisitions and similar events. [Reserved (United States)


    ... 26 Internal Revenue 5 2010-04-01 2010-04-01 false Special rules for mergers, acquisitions and similar events. [Reserved] 1.401(k)-5 Section 1.401(k)-5 Internal Revenue INTERNAL REVENUE SERVICE... Bonus Plans, Etc. § 1.401(k)-5 Special rules for mergers, acquisitions and similar events. [Reserved] [T...

  8. A pharmacological profile of the high-affinity GluK5 kainate receptor

    DEFF Research Database (Denmark)

    Møllerud, Stine; Kastrup, Jette Sandholm Jensen; Pickering, Darryl S


    -hydroxyisoxazol-4-yl)propionate (ATPA), dihydrokainate and (2 S,4 R)−4-methyl-glutamate (SYM2081) have higher affinity at GluK3 compared to GluK5. Since some studies have indicated that GluK5 is associated with various diseases in the central nervous system (e.g. schizophrenia, temporal lobe epilepsy, bipolar...

  9. Shikonin, vitamin K3 and vitamin K5 inhibit multiple glycolytic enzymes in MCF-7 cells. (United States)

    Chen, Jing; Hu, Xun; Cui, Jingjie


    Glycolysis is the most important source of energy for the production of anabolic building blocks in cancer cells. Therefore, glycolytic enzymes are regarded as potential targets for cancer treatment. Previously, naphthaquinones, including shikonin, vitamin K 3 and vitamin K 5 , have been proven to decrease the rate of glycolysis in cancer cells, which is partly due to suppressed pyruvate kinase activity. In the present study, enzymatic assays were performed using MCF-7 cell lysate in order to screen the profile of glycolytic enzymes in cancer cells inhibited by shikonin, vitamin K 3 and vitamin K 5 , in addition to pyruvate kinase. Results revealed that hexokinase, phosphofructokinase-1, fructose bisphosphate aldolase, glyceraldehyde-3-phosphate dehydrogenase and pyruvate kinase produced in the process of glycolysis were inhibited by shikonin, vitamin K 3 and vitamin K 5 . The results indicated that shikonin, vitamin K 3 and vitamin K 5 are chemical inhibitors of glycolytic enzymes in cancer cells and have potential uses in translational medical applications.

  10. Overcoming Pedagogical, Social/Cultural, and Attitudinal Barriers to Technology Integration in K-5 Schools (United States)

    Durff, Lisa


    Technology engages and increases academic achievement for K-5 students, but teachers face attitudinal, social/cultural, and pedagogical barriers when they integrate technology for student learning. Although some teachers overcome these barriers, it remains unclear how they do so. The purpose of this qualitative multiple case study was to analyze…

  11. 29 CFR 71.52 - Specific exemptions pursuant to subsection (k)(5) of the Privacy Act. (United States)


    ... (Investigative Case Tracking Systems/Audit Information Reporting Systems, USDOL/OIG), a system of records... AND ACCESS TO RECORDS UNDER THE PRIVACY ACT OF 1974 Exemption of Records Systems Under the Privacy Act § 71.52 Specific exemptions pursuant to subsection (k)(5) of the Privacy Act. (a) The following systems...

  12. Interaction of Escherichia coli K1 and K5 with Acanthamoeba castellanii Trophozoites and Cysts


    Matin, Abdul; Jung, Suk-Yul


    The existence of symbiotic relationships between Acanthamoeba and a variety of bacteria is well-documented. However, the ability of Acanthamoeba interacting with host bacterial pathogens has gained particular attention. Here, to understand the interactions of Escherichia coli K1 and E. coli K5 strains with Acanthamoeba castellanii trophozoites and cysts, association assay, invasion assay, survival assay, and the measurement of bacterial numbers from cysts were performed, and nonpathogenic E. ...

  13. Genome Sequences of Subcluster K5 Mycobacteriophages AlleyCat, Edugator, and Guillsminger. (United States)

    King, Rodney A; Slowan-Pomeroy, Tina M; Thomas, Jodi E; Ahmed, Tithe; Alexander, Katie L; Biddle, James M; Daniels, Makenzie K; Rowlett, Jenna R; Senay, Taylor E; Rinehart, Claire A; Staples, Amanda K; Rowland, Naomi S; Gaffney, Bobby L; Emmons, Christine B; Hauk, Maya D; Nguyen, Rebecca L; Naegele, Leonard; Strickland, Summer S; Briggs, Laura A; Rush, Alexander N; Saha, Sanghamitra; Sadana, Rachna; Cresawn, Steven G; Russell, Daniel A; Garlena, Rebecca A; Pope, Welkin H; Jacobs-Sera, Deborah; Hatfull, Graham F


    Bacteriophages AlleyCat, Edugator, and Guillsminger were isolated on Mycobacterium smegmatis mc 2 155 from enriched soil samples. All are members of mycobacteriophage subcluster K5, with genomes of 62,112 to 63,344 bp. Each genome contains 92 to 99 predicted protein-coding genes and one tRNA. Guillsminger is the first mycobacteriophage to carry an IS 1380 family transposon. Copyright © 2017 King et al.

  14. Heterotrophic nitrogen removal in Bacillus sp. K5: involvement of a novel hydroxylamine oxidase. (United States)

    Yang, Yunlong; Lin, Ershu; Huang, Shaobin


    An aerobic denitrifying bacterium isolated from a bio-trickling filter treating NOx, Bacillus sp. K5, is able to convert ammonium to nitrite, in which hydroxylamine oxidase (HAO) plays a critical role. In the present study, the performance for simultaneous nitrification and denitrification was investigated with batch experiments and an HAO was purified by an anion-exchange and gel-filtration chromatography from strain K5. The purified HAO's molecular mass was determined by SDS-PAGE and its activity by measuring the change in the concentration of ferricyanide, the electron acceptor. Results showed that as much as 87.8 mg L -1 ammonium-N was removed without nitrite accumulation within 24 hours in the sodium citrate medium at C/N of 15. The HAO isolated from the strain K5 was approximately 71 KDa. With hydroxylamine (NH 2 OH) as a substrate and potassium ferricyanide as an electron acceptor, the enzyme was capable of oxidizing NH 2 OH to nitrite in vitro when the pH varied from 7 to 9 and temperature ranged from 25 °C to 40 °C. This is the first time that an HAO has been purified from the Bacillus genus, and the findings revealed that it is distinctive in its molecular mass and enzyme properties.

  15. Targeted deletion of Atg5 reveals differential roles of autophagy in keratin K5-expressing epithelia

    Energy Technology Data Exchange (ETDEWEB)

    Sukseree, Supawadee [Research Division of Biology and Pathobiology of the Skin, Department of Dermatology, Medical University of Vienna, Vienna (Austria); Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok (Thailand); Rossiter, Heidemarie; Mildner, Michael [Research Division of Biology and Pathobiology of the Skin, Department of Dermatology, Medical University of Vienna, Vienna (Austria); Pammer, Johannes [Institute of Clinical Pathology, Medical University of Vienna, Vienna (Austria); Buchberger, Maria; Gruber, Florian [Research Division of Biology and Pathobiology of the Skin, Department of Dermatology, Medical University of Vienna, Vienna (Austria); Watanapokasin, Ramida [Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok (Thailand); Tschachler, Erwin [Research Division of Biology and Pathobiology of the Skin, Department of Dermatology, Medical University of Vienna, Vienna (Austria); Eckhart, Leopold, E-mail: [Research Division of Biology and Pathobiology of the Skin, Department of Dermatology, Medical University of Vienna, Vienna (Austria)


    Highlights: Black-Right-Pointing-Pointer We generated mice lacking Atg5 and autophagy in keratin K5-positive epithelia. Black-Right-Pointing-Pointer Suppression of autophagy in thymic epithelium was not associated with signs of autoimmunity. Black-Right-Pointing-Pointer Autophagy was required for normal terminal differentiation of preputial gland cells. Black-Right-Pointing-Pointer Autophagy-deficient cells of the preputial glands degraded nuclear DNA prematurely. -- Abstract: Autophagy contributes to the homeostasis of many tissues, yet its role in epithelia is incompletely understood. A recent report proposed that Atg5-dependent autophagy in thymic epithelial cells is essential for their function in the negative selection of self-reactive T-cells and, thus, for the suppression of tissue inflammation. Here we crossed mice carrying floxed alleles of the Atg5 gene with mice expressing the Cre recombinase under the control of the keratin K5 promoter to suppress autophagy in all K5-positive epithelia. The efficiency of autophagy abrogation was confirmed by immunoanalyses of LC3, which was converted to the autophagy-associated LC3-II form in normal but not Atg5-deficient cells, and of p62, which accumulated in Atg5-deficient cells. Mice carrying the epithelium-specific deletion of Atg5 showed normal weight gain, absence of tissue inflammation, and a normal morphology of the thymic epithelium. By contrast, autophagy-deficient epithelial cells of the preputial gland showed aberrant eosinophilic staining in histology and premature degradation of nuclear DNA during terminal differentiation. Taken together, the results of this study suggest that autophagy is dispensable for the suppression of autoimmunity by thymic epithelial cells but essential for normal differentiation of the preputial gland in mice.

  16. Plasminogen fragments K 1-3 and K 5 bind to different sites in fibrin fragment DD. (United States)

    Grinenko, T V; Kapustianenko, L G; Yatsenko, T A; Yusova, O I; Rybachuk, V N


    Specific plasminogen-binding sites of fibrin molecule are located in Аα148-160 regions of C-terminal domains. Plasminogen interaction with these sites initiates the activation process of proenzyme and subsequent fibrin lysis. In this study we investigated the binding of plasminogen fragments K 1-3 and K 5 with fibrin fragment DD and their effect on Glu-plasminogen interaction with DD. It was shown that the level of Glu-plasminogen binding to fibrin fragment DD is decreased by 50-60% in the presence of K 1-3 and K 5. Fragments K 1-3 and K 5 have high affinity to fibrin fragment DD (Kd is 0.02 for K 1-3 and 0.054 μМ for K 5). K 5 interaction is independent and K 1-3 is partly dependent on C-terminal lysine residues. K 1-3 interacts with complex of fragment DD-immobilized K 5 as well as K 5 with complex of fragment DD-immobilized K 1-3. The plasminogen fragments do not displace each other from binding sites located in fibrin fragment DD, but can compete for the interaction. The results indicate that fibrin fragment DD contains different binding sites for plasminogen kringle fragments K 1-3 and K 5, which can be located close to each other. The role of amino acid residues of fibrin molecule Аα148-160 region in interaction with fragments K 1-3 and K 5 is discussed.

  17. Engineering Computer Games: A Parallel Learning Opportunity for Undergraduate Engineering and Primary (K-5 Students

    Directory of Open Access Journals (Sweden)

    Mark Michael Budnik


    Full Text Available In this paper, we present how our College of Engineering is developing a growing portfolio of engineering computer games as a parallel learning opportunity for undergraduate engineering and primary (grade K-5 students. Around the world, many schools provide secondary students (grade 6-12 with opportunities to pursue pre-engineering classes. However, by the time students reach this age, many of them have already determined their educational goals and preferred careers. Our College of Engineering is developing resources to provide primary students, still in their educational formative years, with opportunities to learn more about engineering. One of these resources is a library of engineering games targeted to the primary student population. The games are designed by sophomore students in our College of Engineering. During their Introduction to Computational Techniques course, the students use the LabVIEW environment to develop the games. This software provides a wealth of design resources for the novice programmer; using it to develop the games strengthens the undergraduates

  18. An unexpected oxidation: NaK5Cl2(S2O62 revisited

    Directory of Open Access Journals (Sweden)

    William T. A. Harrison


    Full Text Available The title compound, NaK5Cl2(S2O62 [systematic name: sodium pentapotassium dichloride bis(dithionate], arose as an unexpected product from an organic synthesis that used dithionite (S2O42− ions as a reducing agent to destroy excess permanganate ions. Compared to the previous study [Stanley (1953. Acta Cryst. 6, 187–196], the present tetragonal structure exhibits a root 2a × root 2a × c super-cell due to subtle changes in the orientations of the dithionate anions. The structure can be visualized as a three-dimensional framework of [001] columns of alternating trans-NaO4Cl2 and KO4Cl2 octahedra cross-linked by the dithionate ions with the interstices occupied by KO6Cl2 polyhedra to generate a densely packed three-dimensional framework. The asymmetric unit comprises two sodium ions (site symmetries 4 and -4, four potassium ions (site symmetries = -4, 4, 1 and 1, three chloride ions (site symmetries = 4, 4 and 2 and two half-dithionate ions (all atoms on general positions. Both dithionate ions are completed by crystallographic inversion symmetry. The crystal chosen for data collection was found to be rotationally twinned by 180° about the [100] axis in reciprocal space with a 0.6298 (13:0.3702 (13 domain ratio.

  19. SRNA-2K5, Proton Transport Using 3-D by Monte Carlo Techniques

    International Nuclear Information System (INIS)

    Ilic, Radovan D.


    1 - Description of program or function: SRNA-2K5 performs Monte Carlo transport simulation of proton in 3D source and 3D geometry of arbitrary materials. The proton transport based on condensed history model, and on model of compound nuclei decays that creates in nonelastic nuclear interaction by proton absorption. 2 - Methods: The SRNA-2K5 package is developed for time independent simulation of proton transport by Monte Carlo techniques for numerical experiments in complex geometry, using PENGEOM from PENELOPE with different material compositions, and arbitrary spectrum of proton generated from the 3D source. This package developed for 3D proton dose distribution in proton therapy and dosimetry, and it was based on the theory of multiple scattering. The compound nuclei decay was simulated by our and Russian MSDM models using ICRU 49 and ICRU 63 data. If protons trajectory is divided on great number of steps, protons passage can be simulated according to Berger's Condensed Random Walk model. Conditions of angular distribution and fluctuation of energy loss determinate step length. Physical picture of these processes is described by stopping power, Moliere's angular distribution, Vavilov's distribution with Sulek's correction per all electron orbits, and Chadwick's cross sections for nonelastic nuclear interactions, obtained by his GNASH code. According to physical picture of protons passage and with probabilities of protons transition from previous to next stage, which is prepared by SRNADAT program, simulation of protons transport in all SRNA codes runs according to usual Monte Carlo scheme: (i) proton from the spectrum prepared for random choice of energy, position and space angle is emitted from the source; (ii) proton is loosing average energy on the step; (iii) on that step, proton experience a great number of collisions, and it changes direction of movement randomly chosen from angular distribution; (iv) random fluctuation is added to average energy loss; (v

  20. Measuring psychological distress in older Aboriginal and Torres Strait Islanders Australians: a comparison of the K-10 and K-5. (United States)

    McNamara, Bridgette J; Banks, Emily; Gubhaju, Lina; Williamson, Anna; Joshy, Grace; Raphael, Beverley; Eades, Sandra J


    To assess the cross-cultural validity of two Kessler psychological distress scales (K-10 and K-5) by examining their measurement properties among older Aboriginal and Torres Strait Islanders and comparing them to those in non-Aboriginal individuals from NSW Australia. Self-reported questionnaire data from the 45 and Up Study for 1,631 Aboriginal and 231,774 non-Aboriginal people were used to examine the factor structure, convergent validity, internal consistency and levels of missing data of K-10 and K-5. We found excellent agreement in classification of distress of Aboriginal participants by K-10 and K-5 (weighted kappa=0.87), high internal consistency (Cronbach's alpha K-10: 0.93, K-5: 0.88), and factor structures consistent with those for the total Australian population. Convergent validity was evidenced by a strong graded relationship between the level of distress and the odds of: problems with daily activities due to emotional problems; current treatment for depression or anxiety; and poor quality of life. K-10 and K-5 scales are promising tools for measuring psychological distress among Aboriginal and Torres Strait Islanders aged 45 and over in research and clinical settings. © 2014 Public Health Association of Australia.

  1. Final report on the key comparison CCPR-K5: Spectral diffuse reflectance (United States)

    Nadal, Maria; Eckerle, Kenneth L.; Early, Edward A.; Ohno, Yoshi


    The CCPR K5 key comparison on spectral diffuse reflectance was carried out in the framework of the CIPM Mutual Recognition Arrangement, by 13 national metrology institutes (MMIs) as participants. The participants were CSIR-NML (South Africa), HUT (Finland), IFA-CSIC (Spain), KRISS (Republic of Korea), MSL (New Zealand), NIM (China), NIST (United States of America), NMIJ (Japan), NPL (United Kingdom), NRC (Canada), OMH (Hungary), PTB (Germany) and VNIIOFI (Russia Federation). NIST (USA) piloted the comparison. The aim of this comparison was to check the agreement of measurement of the spectral diffuse reflectance among participants, using the measurement geometry of d/0 or 0/d in the wavelength range of 360 nm to 820 nm at 20 nm increment. The comparison was a star type comparison with the samples provided by the pilot laboratory and with the measurement sequence: Pilot-Participant-Pilot. Spectralon and matte white ceramic tiles were used as the transfer standards. Each participant received three of each type of sample and at least one sample of each type was measured three times on three separate days, and the other two samples were measured once. The report presents the description of the measurement facilities, procedures and uncertainties of all the participants as well as the results of the comparison. Measurement results from the participants and their associated uncertainties were analyzed in accordance with the Guidelines for CCPR Key Comparison Report Preparation, using weighted mean with cut-off. For the calculation of the Key Comparison Reference Value (KCRV), as agreed by the participants, the data of both samples were used for the 460 nm to 820 nm region and only the data of the Spectralon samples were used in the spectral region of 360 nm to 440 nm. The unilateral degrees of equivalence (DoE) calculated for each participant are mostly consistent within the uncertainty (k = 2) of the DoE. This international comparison of spectral diffuse reflectance

  2. Effects of Vitamin K3 and K5 on Daunorubicin-resistant Human T Lymphoblastoid Leukemia Cells. (United States)

    Nakaoka, Eri; Tanaka, Sachiko; Onda, Kenji; Sugiyama, Kentaro; Hirano, Toshihiko


    Anticancer efficacy of vitamin K derivatives on multidrug-resistant cancer cells has been scarcely investigated. The effects of vitamins K3 and K5 on proliferation of human leukemia MOLT-4 cells and on daunorubicin-resistant MOLT-4/DNR cells were estimated by a WST assay. Apoptotic cells were detected by Annexin V and propidium iodide staining, followed by flow cytometry. Vitamins K3 and K5 significantly inhibited proliferation of leukemic cells at 10 and 100 μM (pVitamin K3 induced cell apoptosis at 10 and 100 μM in both MOLT-4 and MOLT-4/DNR cells (pVitamin K5 also increased apoptotic cells, while rather inducing necrotic cell death. Vitamins K3 and K5 suppress MOLT-4 and MOLT-4/DNR cell-proliferation partially through induction of apoptosis, and these vitamin derivatives can overcome drug resistance due to P-glycoprotein expression. Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

  3. Moving the School and Dancing Education: Case Study Research of K-5 Students' Experiences in a Dance Residency (United States)

    Leonard, Alison E.


    This dissertation chronicles the qualitative case study of a dance artist-in-residence at a diverse and inclusive K-5 school in an urban district, integrating science, social studies, physical education, music, and visual arts school curriculum and culminating in two public performances. This study focused on how students made meaning through this…

  4. K5/K14-positive cells contribute to salivary gland-like breast tumors with myoepithelial differentiation

    DEFF Research Database (Denmark)

    Boecker, Werner; Stenman, Goeran; Loening, Thomas


    different cell lineages and define their cellular hierarchy in tumors with myoepithelial differentiation. isTILT analysis of a series of 28 breast, salivary, and lacrimal gland tumors, including pleomorphic adenomas (n=8), epithelial-myoepithelial tumors (n=9), and adenoid cystic carcinomas (n=11) revealed...... heterologeous cell differentiations such as squamous and mesenchymal progenies. p63 was co-expressed with K5/K14 in basal-like progenitor cells, myoepithelial, and squamous cells but not in glandular cells. Our results show that the corresponding counterpart tumors of breast and salivary/lacrimal glands have....... For that reason, we performed an in situ triple immunofluorescence lineage/differentiation tracing (isTILT) and qRT-PCR study of basal (K5/K14), glandular (K7/K8/18), and epidermal-specific squamous (K10) keratins, p63, and smooth muscle actin (SMA; myoepithelial marker) with the aim to construct and trace...

  5. Transport mean free path in K5Bi1-xNdx(MoO4)4 laser crystal powders

    International Nuclear Information System (INIS)

    Illarramendi, M A; Aramburu, I; Fernandez, J; Balda, R; Al-Saleh, M


    In this work, we calculate in two different ways the transport mean free paths in K 5 Bi 1-x Nd x (MoO 4 ) 4 (x = 0.05, 0.2, 1) laser crystal powders by using the diffuse spectral reflectance and transmittance of the powders and the absorption coefficient of the crystal materials. The theoretical calculations have been made by assuming a diffusive propagation of light in these materials. Similar results have been obtained from both methods

  6. Inter-RMO Key Comparison EUROMET.L-K5.2004: Calibration of a step gauge

    DEFF Research Database (Denmark)

    Prieto, Emilio; Brown, Nicholas; Lassila, Antti


    The results of the inter-RMO key comparison EUROMET.L-K5.2004 on the calibration of a step gauge are reported. Eighteen National Metrology Institutes and one Designated Institute from four different metrological regions all over the world participated in this comparison which lasted three years...... value. Due to the significant instability of the step it was also considered an artefact uncertainty. The reported uncertainties ranged from 0.045 µm to 1.2 µm (k = 1). The uncertainty of the artefact ranged from 0.018 µm (for the 20 mm face) to 0.176 µm (for the 400 mm face). The compatibility of all...... participants for measuring step gauges was demonstrated with the only exception of a participant showing very high systematic (both positive and negative) errors. Five participants communicated higher uncertainties than the corresponding approved CMCs. A set of Recommendations and Actions were agreed therefore...

  7. Report of the key-comparison of spectral diffuse reflectance (EURAMET.PR-K5) (Ref. 619) (United States)

    Andor, György; Gál, Péter


    This report details the final results of the EURAMET comparison on regular spectral transmittance carried out between 2006 and 2016. The aim of this comparison was to check the agreement of measurement of the spectral diffuse reflectance among participants, using the measurement geometry of d/0 or 0/d in the wavelength range of 360 nm to 780 nm at 20 nm increment. We used a star type comparison: first the participants sent their samples to the pilot, than the pilot measured all the samples of the participants and sent them back. The participants measured the samples and sent them to the pilot for control measurement. Six standards were used as reference standards in order to maintain the scale during the comparison. These were three samples of BCR-406 opal glasses (BCR 30506; BCR 30303; BCR 30704), an MC20 Russian opal glass (MC 4777) and two samples made of pressed halon (polytetrafluoroethylene) powder (halon 2007A; halon 2007C). These six samples were designated as the Comparison reference standards. The diffuse reflectance was initially measured on the OMH (BFKH) absolute reflectometer. The link to the CCPR-K5 results was BFKH, and the check on BFKH was the PTB results who also participated in the CCPR-K5 comparison. The participants were GUM, INM, LNE, METAS, BFKH, PTB, SP. Main text To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database The final report has been peer-reviewed and approved for publication by the CCPR, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

  8. Collecting the Missing Piece of the Puzzle: The Wind Temperatures of Arcturus (K2 III) and Aldeberan (K5 III) (United States)

    Harper, Graham


    Unravelling the poorly understood processes that drive mass loss from red giant stars requires that we empirically constrain the intimately coupled momentum and energy balance. Hubble high spectral resolution observations of wind scattered line profiles, from neutral and singly ionized species, have provided measures of wind acceleration, turbulence, terminal speeds, and mass-loss rates. These wind properties inform us about the force-momentum balance, however, the spectra have not yielded measures of the much needed wind temperatures, which constrain the energy balance.We proposed to remedy this omission with STIS E140H observations of the Si III 1206 Ang. resonance emission line for two of the best studied red giants: Arcturus (alpha Boo: K2 III) and Aldebaran (alpha Tau: K5 III), both of which have detailed semi-empirical wind velocity models. The relative optical depths of wind scattered absorption in Si III 1206 Ang., O I 1303 Ang. triplet., C II 1335 Ang., and existing Mg II h & k and Fe II profiles give the wind temperatures through the thermally controlled ionization balance. The new temperature constraints will be used to test existing semi-empirical models by comparision with multi-frequency JVLA radio fluxes, and also to constrain the flux-tube geometry and wave energy spectrum of magnetic wave-driven winds.

  9. Inhibition of B16-BL6 melanoma lung colonies by semisynthetic sulfaminoheparosan sulfates from E. coli K5 polysaccharide. (United States)

    Poggi, Andreina; Rossi, Cosmo; Casella, Nicola; Bruno, Cristiana; Sturiale, Luisella; Dossi, Carla; Naggi, Annamaria


    Heparin (H), heparan sulfate (HS), and related glycosaminoglycans can inhibit cancer cell invasion, possibly due to their ability to interact with vascular growth factors, adhesion molecules, endoglycosidases, and signaling proteins, in addition to the well-known effects on the clotting system. We evaluated the antitumor activity of a series of semisynthetic sulfaminoheparosan sulfates (SAHSs) with different degree and distribution of sulfates, obtained by chemical modifications of the E. coli K5 polysaccharide, namely type A, B, and C compounds. B16-BL6 melanoma cells (10 5 cells/mouse) were injected intravenously (i.v.) in a lateral tail vein of C57BL6 mice at a dose of 0.5 mg/ mouse together with test compounds. Tumor lung nodules were significantly reduced as compared with controls only by H (95.5 +/- 1.0% inhibition), SAHS-2 (84.2 +/- 5.0% inhibition), and SAHS-4 (91.1 +/- 4.2% inhibition), among compounds tested. SAHS-2 and SAHS-4 are type B compounds, with a sulfate/carboxylate ratio similar to that of H. A typical mammalian HS showed only 54.8% inhibition. Supersulfated low-molecular-weight heparin and heparan sulfate (ssLMWH and ssLMWHS) showed an activity similar to that of unfractionated compounds. H and SAHS-4 inhibited dose dependently B16-BL6 lung colonies, with IC-50 values of 0.05 and 0.1 mg/mouse, respectively. The relationship with ex vivo anticoagulant potency was evaluated by activated partial thromboplastin time (aPTT) on mouse plasma at different time intervals after i.v. injection (0.1 to 0.5 mg/mouse) of the compound. H showed a dose-dependent anticoagulant activity lasting up to 2 hours, whereas SAHS-4 showed a potent anticoagulant effect only at a dose of 0.5 mg/mouse. Accordingly, H but not SAHS-4 consistently inhibited B16-BL6 lung colonies when given 1 hour before tumor cells. SAHS-4 derivatives, with different size and/or affinity depleted of AT binding sites, showed an inhibitory effect on B16-BL6 melanoma similar to that of SAHS-4

  10. Structures of three different neutral polysaccharides of Acinetobacter baumannii, NIPH190, NIPH201, and NIPH615, assigned to K30, K45, and K48 capsule types, respectively, based on capsule biosynthesis gene clusters. (United States)

    Shashkov, Alexander S; Kenyon, Johanna J; Arbatsky, Nikolay P; Shneider, Mikhail M; Popova, Anastasiya V; Miroshnikov, Konstantin A; Volozhantsev, Nikolay V; Knirel, Yuriy A


    Neutral capsular polysaccharides (CPSs) were isolated from Acinetobacter baumannii NIPH190, NIPH201, and NIPH615. The CPSs were found to contain common monosaccharides only and to be branched with a side-chain 1→3-linked β-d-glucopyranose residue. Structures of the oligosaccharide repeat units (K units) of the CPSs were elucidated by 1D and 2D (1)H and (13)C NMR spectroscopy. Novel CPS biosynthesis gene clusters, designated KL30, KL45, and KL48, were found at the K locus in the genome sequences of NIPH190, NIPH201, and NIPH615, respectively. The genetic content of each gene cluster correlated with the structure of the CPS unit established, and therefore, the capsular types of the strains studied were designated as K30, K45, and K48, respectively. The initiating sugar of each K unit was predicted, and glycosyltransferases encoded by each gene cluster were assigned to the formation of the linkages between sugars in the corresponding K unit. Copyright © 2015 Elsevier Ltd. All rights reserved.

  11. Characterization of Camptothecin-induced Genomic Changes in the Camptothecin-resistant T-ALL-derived Cell Line CPT-K5

    DEFF Research Database (Denmark)

    Kjeldsen, Eigil; Nielsen, Christine J F; Roy, Amit


    -K5 and its parental cell line. We identified copy number alterations affecting genes important for maintaining genome integrity and reducing CPT-induced DNA damage. We show for the first time that short tandem repeats are targets for TOP1 cleavage, that can be differentially stimulated by CPT.......Acquisition of resistance to topoisomerase I (TOP1)-targeting camptothecin (CPT) derivatives is a major clinical problem. Little is known about the underlying chromosomal and genomic mechanisms. We characterized the CPT-K5 cell line expressing mutant CPT-resistant TOP1 and its parental T......-cell derived acute lymphoblastic leukemia CPT-sensitive RPMI-8402 cell line by karyotyping and molecular genetic methods, including subtractive oligo-based array comparative genomic hybridization (soaCGH) analysis. Karyotyping revealed that CPT-K5 cells had acquired additional structural aberrations...

  12. Vitamins K2, K3 and K5 exert antitumor effects on established colorectal cancer in mice by inducing apoptotic death of tumor cells. (United States)

    Ogawa, Mutsumi; Nakai, Seiji; Deguchi, Akihiro; Nonomura, Takako; Masaki, Tsutomu; Uchida, Naohito; Yoshiji, Hitoshi; Kuriyama, Shigeki


    Although a number of studies have shown that vitamin K possesses antitumor activities on various neoplastic cell lines, there are few reports demonstrating in vivo antitumor effects of vitamin K, and the antitumor effect on colorectal cancer (CRC) remains to be examined. Therefore, antitumor effects of vitamin K on CRC were examined both in vitro and in vivo. Vitamins K2, K3 and K5 suppressed the proliferation of colon 26 cells in a dose-dependent manner, while vitamin K1 did not. On flow cytometry, induction of apoptosis by vitamins K2, K3 and K5 was suggested by population in sub-G1 phase of the cell cycle. Hoechst 33342 staining and a two-color flow cytometric assay using fluorescein isothiocyanate-conjugated annexin V and propidium iodide confirmed that vitamins K2, K3 and K5 induced apoptotic death of colon 26 cells. Enzymatic activity of caspase-3 in colon 26 cells was significantly up-regulated by vitamins K2, K3 and K5. The pan-caspase inhibitor, benzyloxycarbonyl-Val-Ala-Asp-fluoromethyl ketone, substantially prevented vitamin K-mediated apoptosis. In vivo study using syngeneic mice with subcutaneously established colon 26 tumors demonstrated that intravenous administration of vitamins K2, K3 and K5 significantly suppressed the tumor growth. The number of apoptotic tumor cells was significantly larger in the vitamin K-treated groups than in the control group. These results suggest that vitamins K2, K3 and K5 exerted effective antitumor effects on CRC in vitro and in vivo by inducing caspase-dependent apoptotic death of tumor cells, suggesting that these K vitamins may be promising agents for the treatment of patients with CRC.

  13. Evidence of a massive planet candidate orbiting the young active K5V star BD+20 1790 (United States)

    Hernán-Obispo, M.; Gálvez-Ortiz, M. C.; Anglada-Escudé, G.; Kane, S. R.; Barnes, J. R.; de Castro, E.; Cornide, M.


    Context. BD+20 1790 is a young active, metal-rich, late-type K5Ve star. We have undertaken a study of stellar activity and kinematics for this star over the past few years. Previous results show a high level of stellar activity, with the presence of prominence-like structures, spots on the surface, and strong flare events, despite the moderate rotational velocity of the star. In addition, radial velocity variations with a semi-amplitude of up to 1 km s-1 were detected. Aims: We investigate the nature of these radial velocity variations, in order to determine whether they are due to stellar activity or the reflex motion of the star induced by a companion. Methods: We have analysed high-resolution echelle spectra by measuring stellar activity indicators and computing radial velocity (RV) and bisector velocity spans. Two-band photometry was also obtained to produce the light curve and determine the photometric period. Results: Based upon the analysis of the bisector velocity span, as well as spectroscopic indices of chromospheric indicators, Ca ii H & K, Hα, and taking the photometric analysis into account, we report that the best explanation for the RV variation is the presence of a substellar companion. The Keplerian fit of the RV data yields a solution for a close-in massive planet with an orbital period of 7.78 days. The presence of the close-in massive planet could also be an interpretation for the high level of stellar activity detected. Since the RV data are not part of a planet search programme, we can consider our results as a serendipitous evidence of a planetary companion. To date, this is the youngest main sequence star for which a planetary candidate has been reported. Based on observations collected at the German-Spanish Astronomical Center, Calar Alto, jointly operated by the Max-Planck-Institut für Astronomie Heidelberg and the Instituto de Astrofísica de Andalucía (CSIC). Based on observations made with the Italian Telescopio Nazionale Galileo

  14. Modeling Math Growth Trajectory--An Application of Conventional Growth Curve Model and Growth Mixture Model to ECLS K-5 Data (United States)

    Lu, Yi


    To model students' math growth trajectory, three conventional growth curve models and three growth mixture models are applied to the Early Childhood Longitudinal Study Kindergarten-Fifth grade (ECLS K-5) dataset in this study. The results of conventional growth curve model show gender differences on math IRT scores. When holding socio-economic…

  15. An analysis of the relationship between K--5 elementary school teachers' perceptions of principal instructional leadership and their science teaching efficacy (United States)

    Clark, Ian

    The purpose of this study is to analyze the relationship between K-5 elementary school teachers' perceptions of principal instructional leadership and their science teaching efficacy. The influence of background variables on both leadership and efficacy is also analyzed. A sequential mixed methods approach was used in this study. The survey sample was comprised of teachers in the elementary divisions of schools from the nine international school regional associations. Teacher participation was obtained through an email containing an online survey link. Following the analysis of survey responses (N=356), in-depth interviews (N=17) were conducted. Reliability for the instructional leadership scale was found to be .94 (coefficient alpha) and .69 for the personal science teaching efficacy (PSTE) scale. The results show a significant correlation between elementary school teachers' perceptions of principal instructional leadership and their PSTE levels, with the most significant correlation that between the study of a science-related major or minor at college and higher PSTE scores. Strong correlations were also found between PSTE levels and having principals who discussed goals at faculty meetings, participated in science curricular review, supported recognition of student progress, encouraged new skills and concepts, discussed student progress with faculty, and used assessments to see science progress towards easily understood goals. PSTE levels were also higher in schools where principals had grade or school level science coordinators in place and where they supported the use of science kits.

  16. Radionuclide Incorporation in Secondary Crystalline Minerals Resulting from Chemical Weathering of Selected Waste Glasses: Progress Report: Task kd.5b

    International Nuclear Information System (INIS)

    Mattigod, Shas V.; Serne, R. Jeffrey; Legore, Virginia L.; Parker, Kent E.; Orr, Robert D.; McCready, David E.; )


    Experiments were conducted by Pacific Northwest National Laboratory to evaluate potential incorporation of radionuclides in secondary mineral phases that form from weathering vitrified nuclear waste glasses. These experiments were conducted as part of the Immobilized Low-Activity Waste-Performance Assessment (ILAW-PA) to generate data on radionuclide mobilization and transport in a near-field environment of disposed vitrified wastes. The results of these experiments demonstrated that radionuclide sequestration can be significantly enhanced by promoting the formation of cage structured minerals such as sodalite from weathering glasses. These results have important implications regarding radionuclide sequestration/mobilization aspects that are not currently accounted for in the ILAW PA. Additional studies are required to confirm the results and to develop an improved understanding of the mechanisms of sequestration of radionuclides into the secondary and tertiary weathering products o f the ILAW glass to help refine how contaminants are released from the near-field disposal region out into the accessible environment. Of particular interest is to determine whether the contaminants remain sequestered in the glass weathering products for hundreds to thousands of years. If the sequestration can be shown to continue for long periods, another immobilization process can be added to the PA analysis and predicted risks should be lower than past predictions

  17. The CO2 Abundance in Comets C2012 K1 (PanSTARRS), C2012 K5 (LINEAR), and 290P Jager as Measured with Spitzer (United States)

    McKay, Adam J.; Kelley, Michael S.P.; Cochran, Anita L.; Bodewits, Dennis; DiSanti, Michael A.; Dello Russo, Neil; Lisse, Carey M.


    Carbon dioxide is one of the most abundant ices present in comets and is therefore important for understanding cometary composition and activity. We present analysis of observations of CO2 and [O I] emission in three comets to measure the CO2 abundance and evaluate the possibility of employing observations of [O I] emission in comets as a proxy for CO2. We obtained NIR imaging sensitive to CO2 of comets C/2012 K1 (PanSTARRS), C/2012 K5 (LINEAR), and 290P/Jager with the IRAC instrument on Spitzer. We acquired observations of [O I] emission in these comets with the ARCES echelle spectrometer mounted on the 3.5-m telescope at Apache Point Observatory and observations of OH with the Swift observatory (PanSTARRS) and with Keck HIRES (Jager). The CO2/H2O ratios derived from the Spitzer images are 12.6 +/- 1.3% (PanSTARRS), 28.9 +/- 3.6% (LINEAR), and 31.3 +/- 4.2% (Jager). These abundances are derived under the assumption that contamination from CO emission is negligible. The CO2 abundance for PanSTARRS is close to the average abundance measured in comets at similar heliocentric distance to date, while the abundances measured for LINEAR and Jager are significantly larger than the average abundance. From the coma morphology observed in PanSTARRS and the assumed gas expansion velocity, we derive a rotation period for the nucleus of about 9.2 h. Comparison of H2O production rates derived from ARCES and Swift data, as well as other observations, suggest the possibility of sublimation from icy grains in the inner coma. We evaluate the possibility that the [O I] emission can be employed as a proxy for CO2 by comparing CO2/H2O ratios inferred from the [O I] lines to those measured directly by Spitzer. We find that for PanSTARRS we can reproduce the observed CO2 abundance to an accuracy of approximately 20%. For LINEAR and Jager, we were only able to obtain upper limits on the CO2 abundance inferred from the [O I] lines. These upper limits are consistent with the CO2 abundances

  18. Vitamins K2, K3 and K5 exert in vivo antitumor effects on hepatocellular carcinoma by regulating the expression of G1 phase-related cell cycle molecules. (United States)

    Kuriyama, Shigeki; Hitomi, Misuzu; Yoshiji, Hitoshi; Nonomura, Takako; Tsujimoto, Tatsuhiro; Mitoro, Akira; Akahane, Takami; Ogawa, Mutsumi; Nakai, Seiji; Deguchi, Akihiro; Masaki, Tsutomu; Uchida, Naohito


    A number of studies have shown that various vitamins K, specifically vitamin K2, possessed antitumor activity on various types of rodent- and human-derived neoplastic cell lines. However, there are only a small number of reports demonstrating in vivo antitumor effects of vitamins K. Furthermore, the mechanism of antitumor effects of vitamins K still remains to be examined. In the present study, we examined the antitumor effects of vitamins K2, K3 and K5 on PLC/PRF/5 human hepatocellular carcinoma (HCC) cells in vivo. Furthermore, to examine the mechanism of antitumor actions of these vitamins K, mRNA expression levels of various G1 phase-related cell cycle molecules were evaluated by using a real-time reverse transcription-polymerase chain reaction (RT-PCR) method. HCC-bearing animals were produced by implanting PLC/PRF/5 cells subcutaneously into athymic nude mice, and drinking water containing vitamin K2, K3 or K5 was given to the animals. Treatments with vitamins K2, K3 and K5 were shown to markedly inhibit the growth of HCC tumors. To examine the mechanism of in vivo antitumor effects of vitamins K, total RNA was extracted from HCC tumors, and the expression of G1 phase-related cell cycle molecules was quantitatively examined. Real-time RT-PCR demonstrated that the expression of the cell cycle-driving molecule, cyclin-dependent kinase 4 (Cdk4), in HCC was significantly reduced by the treatments with vitamin K2, K3 and K5. Conversely, the expression of the cell cycle-suppressing molecules, Cdk inhibitor p16INK4a and retinoblastoma, in HCC was significantly enhanced by the treatments with vitamins K2, K3 and K5. These results indicate that vitamins K2, K3 and K5 exert antitumor effects on HCC by regulating the expression of G1 phase-related cell cycle molecules. These results also indicate that vitamins K2, K3 and K5 may be useful agents for the treatment of patients with HCC.

  19. Multicolor immunofluorescence reveals that p63- and/or K5-positive progenitor cells contribute to normal breast epithelium and usual ductal hyperplasia but not to low-grade intraepithelial neoplasia of the breast. (United States)

    Boecker, Werner; Stenman, Göran; Schroeder, Tina; Schumacher, Udo; Loening, Thomas; Stahnke, Lisa; Löhnert, Catharina; Siering, Robert Michael; Kuper, Arthur; Samoilova, Vera; Tiemann, Markus; Korsching, Eberhard; Buchwalow, Igor


    We contend that knowledge about the cellular composition of normal breast epithelium is a prerequisite for understanding proliferative breast disease. Against this background, we used multicolor immunofluorescence to study normal breast epithelium and two types of intraepithelial proliferative breast lesion for expression of the p63, basal keratin K5, glandular keratin K8/18, SMA, ER-alpha, and Ki67. We studied eight normal breast epithelium samples, 12 cases of usual ductal hyperplasia, and 33 cases of low-grade intraepithelial neoplasia (9 flat epithelial atypia, 14 low-grade ductal carcinoma in situ and 10 cases of lobular neoplasia). Usual ductal hyperplasia showed striking similarity to normal luminal breast epithelium including p63+ and/or K5+ luminal progenitor cells and the full spectrum of luminal progeny cells. In normal breast epithelium and usual ductal hyperplasia, expression of ER-alpha was associated with lack of expression of the proliferation antigen Ki67. In contrast, we found in both types of low-grade intraepithelial neoplasia robust expression of keratin K8/18 and a positive association between ER-alpha and Ki67 expression. However, these lesions were consistently negative for p63 and/or K5. Our observational study supports the view that usual ductal hyperplasia and low-grade intraepithelial neoplasia are different entities rather than part of a spectrum of the same disease. We propose a new operational model of cell differentiation that may serve to better understand correlations between normal breast epithelium and proliferative breast diseases. From our data we conclude that p63+ and/or K5+ progenitor cells contribute to maintenance of normal epithelium and usual ductal hyperplasia, but not to low-grade intraepithelial neoplasia of the breast.

  20. Zvýšení ročního využití parního práškového kotle K5 ve ŽĎAS, a. s


    Kubiš, David


    Tato diplomová práce se zabývá vyšším využitím kotle K5 v teplárně ŽĎAS, a.s. v přechodném období, kdy vlivem snížených odběrů dochází k přehřívání zpáteční větve horkovodu a je nutnost přejíždět na jiný kotel. Cílem práce je omezit přejíždění z kotle K5 na kotel K3 a zvýšit jeho časové využití v přechodném období pomocí ekonomicky únosného technického řešení. Na začátku práce je popsána teplárna ŽĎAS, a.s. a její vybavení. Následují čtyři metody výpočtů zvýšení ročního využití kotle K5. A to...

  1. Discovery Handbook for Grades K-5. (United States)

    Johnson, Terry

    This resource packet addresses the need for interpretive materials of the prehistory and early history of the state of Arkansas. These curriculum-focused activities, based on flyers and books created by the Arkansas Archaeology Survey, encourage integration of archaeological and historical study into classroom instruction. The teacher's guide is…

  2. Solvothermal indium fluoride chemistry: Syntheses and crystal structures of K5In3F14, β-(NH4)3InF6 and [NH4]3[C6H21N4]2[In4F21

    International Nuclear Information System (INIS)

    Jayasundera, Anil C.A.; Goff, Richard J.; Li Yang; Finch, Adrian A.; Lightfoot, Philip


    The solvothermal syntheses and crystal structures of three indium fluorides are presented. K 5 In 3 F 14 (1) and β-(NH 4 ) 3 InF 6 (2) are variants on known inorganic structure types chiolite and cryolite, respectively, with the latter exhibiting a complex and apparently novel structural distortion. [NH 4 ] 3 [C 6 H 21 N 4 ] 2 [In 4 F 21 ] (3) represents a new hybrid composition displaying a unique trimeric metal fluoride building unit. - Graphical abstract: Solvothermal synthesis has been used to prepare three indium fluorides, including a novel hybrid material containing a unique [In 3 F 15 ] trimer templated by tren.

  3. Decision Making Perspectives of Young Children (K-5). (United States)

    Rogers, Linda J.; McDonald, Linda L.


    Applies Blumer's theory of symbolic interactionism to explore how normal children make meaning of their decision making world, both of their own acts and the labyrinthine world adults structure for them. Schema/narratives on friendship, sports, adults, and school reveal knowledge making strategies defined by immediate experiences and a capacity to…

  4. A Poisson process approximation for generalized K-5 confidence regions (United States)

    Arsham, H.; Miller, D. R.


    One-sided confidence regions for continuous cumulative distribution functions are constructed using empirical cumulative distribution functions and the generalized Kolmogorov-Smirnov distance. The band width of such regions becomes narrower in the right or left tail of the distribution. To avoid tedious computation of confidence levels and critical values, an approximation based on the Poisson process is introduced. This aproximation provides a conservative confidence region; moreover, the approximation error decreases monotonically to 0 as sample size increases. Critical values necessary for implementation are given. Applications are made to the areas of risk analysis, investment modeling, reliability assessment, and analysis of fault tolerant systems.

  5. Integration of Collaborative Learning in Grade K-5 EFL Classrooms (United States)

    Shahamat, Ailar; Mede, Enisa


    This study investigated the effectiveness of integrating collaborative learning in Turkish elementary (primary) classrooms where English is acquired as a foreign language. Specifically, it aimed at shedding light on how the participating students and teachers perceive such language classes, what are the effects of integrating this particular…

  6. Integrating the Performing Arts in Grades K-5 (United States)

    Rajan, Rekha S.


    Research documents that the arts boost learning, build confidence, and motivate students to participate in class. How do we keep the performing arts alive in this era of increased accountability and decreased funding? Rekha S. Rajan sets the stage for a creative and practical solution with detailed, concrete examples of how to integrate the…

  7. Responsive Guided Reading in Grades K-5: Simplifying Small-Group Instruction (United States)

    Berne, Jennifer; Degener, Sophie C.


    Guided reading is a staple of elementary literacy instruction, yet planning and conducting reading groups can be time consuming and challenging. This hands-on book presents an innovative approach to guided reading that is manageable even for teachers who are new to small-group, differentiated reading instruction. Numerous classroom examples…

  8. HAT-P-68b: A Transiting Hot Jupiter Around a K5 Dwarf Star (United States)

    Lindor, Bethlee; Hartman, Joel D.


    One of the main goals of the astrophysical society has been to detect sources of life outside of Earth. To aid this search, astronomers have spent the last 2 decades focused on the discovery and characterization of exoplanets. The most effective method for doing so has been transit photometry, wherein we measure the brightness of stars over periods of time. These measurements, or light curves, are later analyzed for dips in brightness caused by objects passing in front of the star. However, variations in these time series can also occur due to non-planetary systems and a meticulous process is needed to distinguish the planets from the various false positives that are detected. HATNet is one of many surveys involved in this endeavor, and in this work I analyze HAT-P-68. First, I model the system as a single star with a transiting planet and derive estimates of the stellar and planetary physical parameters. I also model HAT-P-68 as a number of a false positives such as a pair of stars in an eclipsing binary blended with a background star, and a planet-sized star orbiting a Sun-like star. In order to rule out the possibility that HAT-P-68 is a blend, I carried out a statistical blend analysis of the photometric data and find that all blend models tested can be ruled out. Thus, I conclude that HAT-P-68 is a system with a transiting hot jupiter and consider what future observations would be most promising to further characterize the system.

  9. Progress Report Natsurv 1 K5_2285/3 The Malt Brewing Industry

    CSIR Research Space (South Africa)

    Ramukhwatho, Fhumulani R


    Full Text Available of brewing process cycle The generic brewing processes for beer production (Varman and Sutherland, 1994; Unicer, SA, 2005) 5 Breweries Classification Large = 10 Medium = ? Small = 77 Per Barrel produced: Large: > 6 000 000 Medium: ≥ 15 000 ≤ 6... in study 10 Loci of Malt Breweries in SA 11 Key Brewing Processes Brewing Fermentation Storage Bottling Barrelling 12 Preliminary Results from Questionnaire/ literature • Results based on 8 responses (breweries) • Data to date was synthesized...

  10. Remedial Investigation Badger Army Ammunition Plant, Baraboo, Wisconsin. Volume 5. Appendix K.5 (United States)


    4 -4 -4 -4 -4.4 -4r44 4 4-4 -4 ~ 4 .010 -4 000000000000000000000000 000000000000000000000000000 *d -4 8 (a *~ SI * 4JI j -OZ2~404 ZtM0G. .P4000I 4bd ...000000000000000 4.3 tD ~-O-.4- 4-%iCiIO f ~~a to -: u t A w 00000000000000000000000000 )1 310000000000000000000000000000 m o >.,V 0i. 01 00 01 r4rle4r e4


    Directory of Open Access Journals (Sweden)

    Jonathan G. Wagner


    Full Text Available A 52-year-old African American male with a long history of poorly controlled hypertension presented to the emergency department (ED with two days of genital edema and pain. During ED work-up, the patient developed sudden onset of non-pitting, non-pruritic, and non-urticarial upper lip edema. Review of his antihypertensive medication list revealed that he normally took benazepril, highly suggestive of a diagnosis of angiotensin-converting-enzyme inhibitor-related angioedema (ACEIRA. We present the first reported case of penile ACEI-RA that progressed to involve the oropharynx. The ED management of the condition and some of the newer treatment options available for ACEIRA is also briefly discussed.

  12. K-5 mentor teachers' journeys toward reform-oriented science within a professional development school context (United States)

    Manno, Jacqueline L.

    Reform-oriented science teaching with a specific focus on evidence and explanation provides a student-centered learning environment which encourages children to question, seek answers to those questions, experience phenomena, share ideas, and develop explanations of science concepts based on evidence. One of the ways schools have risen to meet the challenge of ever-increasing demands for success in science and all other curricular areas has been in the development of professional development schools (PDSs). Dedicated to the simultaneous renewal of schools and teacher education programs, the structure of a PDS plays a significant role in the change process. The purpose of this research study was to investigate the nature of change in mentor teachers' beliefs and pedagogical practices toward science teaching in the elementary school as conveyed through their own "stories of practice". The major research questions that guided the study were: (1) How do mentor teachers describe their science teaching practices and how have they changed as a result of participation in PDS? (a) In what ways do PDS mentor teachers' descriptions of practice reflect contemporary reform ideas and practices in science education? (b) To what extent do their stories emphasize technical aspects of teaching versus epistemological changes in their thinking and knowledge? (c) How is student learning in science reflected in teachers' stories of practice? (2) What is the relationship between the levels and types of involvement in PDS to change in thinking about and practices of teaching science? (3) What is the depth of commitment that mentors convey about changes in science teaching practices? Using case study design, the research explored the ways experienced teachers, working within the context of a PDS community, described changes in the ways they think about and teach science. The connection to the issue of change in teaching practices grew out of interest in understanding the relationship between mentor teachers' engagement in PDS activities and their thinking about classroom practice. The main focus of this research study was on change in science teaching within the context of a professional development school. PDS literature and current literature on the learning and teaching of science in grades K-8 provided a theoretical orientation to guide the research. Additionally, literature on the process of change in schools helped to narrow the focus of the study while using a lens of situated learning provided additional insight. Analysis of the interview data generated seven assertions that captured the nature of the change process of mentor teachers. Science-specific professional development as well as strong support and encouragement within an active community of learners played significant roles in the transformation of mentor teachers from traditional or activity-based science teachers into educators who use reform-oriented methods and a lens of evidence and explanation to guide their science teaching. Mentor teachers acknowledged an increase in student interest and excitement toward science as a result of these changes in science teaching practices. In addition, data revealed that mentor teachers remained committed to their changed practice after several years. By examining the change process of mentor teachers in a PDS environment, findings from this study are discussed based on implications regarding the factors that contribute to and affect change as reform-oriented practices are implemented in science, a curricular area that is often neglected by elementary teachers.

  13. Integrating Engineering into Delaware's K-5 Classrooms: A Study of Pedagogical and Curricular Resources (United States)

    Grusenmeyer, Linda Huey

    This study examines the personal and curricular resources available to Delaware's elementary teachers during a time of innovative curriculum change, i.e., their knowledge, goals and beliefs regarding elementary engineering curriculum and the pedagogical support to teach two Science and Engineering Practices provided by science teaching materials. Delaware was at the forefront of K-12 STEM movement, first to adopt statewide elementary curriculum materials to complement existing science units, and one of the first to adopt the new science standards--Next Generation Science Standards. What supports were available to teachers as they adapted and adopted this new curriculum? To investigate this question, I examined (1) teachers' beliefs about engineering and the engineering curriculum, and (2) the pedagogical supports available to teachers in selected science and engineering curriculum. Teachers' knowledge, goals, and beliefs regarding Delaware's adoption of new elementary engineering curriculum were surveyed using an adapted version of the Design, Engineering, and Technology Survey (Hong, Purser, & Gardella, 2011; Yaser, Baker, Carpius, Krauss, & Roberts, 2006). Also, three open ended questions sought to reveal deeper understanding of teacher knowledge and understanding of engineering; their concerns about personal and systemic resources related to the new curriculum, its logistics, and feasibility; and their beliefs about the potential positive impact presented by the engineering education initiative. Teacher concerns were analyzed using the Concerns-Based Adoption Model (Hall & Hord, 2010). Lay understandings of engineering were analyzed by contrasting naive representations of engineering with three key characteristics of engineering adapted from an earlier study (Capobianco Diefes-Dux, Mena, & Weller, 2011). Survey findings for teachers who had attended training and those who have not yet attended professional development in the new curriculum were compared with few notable differences. Almost all elementary teacher respondents were familiar with engineering and able to define it using one or more key characteristics. They valued the inclusion of engineering in the elementary curriculum; however trained and untrained teachers reported they were not confident about teaching it and were unaware of the new standards related to engineering. Teachers saw potential advantages or benefits of the new curriculum as helping improve science and math understanding, an opportunity to increase vocational awareness, and engaging students and motivating them to learn. Most teachers saw similar barriers to implementation- lack of teacher knowledge, lack of time to learn about engineering and how to teach engineering, and lack of administrative support. Almost all were open to additional in-service training to learn more about this new curriculum. Three fifth grade science units were examined for evidence of teacher pedagogical support in teaching two Science and Engineering Practices (SEP) advocated by the Next Generation Science Standards. An analytic framework was developed based upon two NGSS SEPs: Asking questions, defining problems and Engaging in argument from evidence. Findings revealed that the kits varied greatly in their pedagogical approaches to the two SEPs and differences might be explained by each kit's underlying orientations to the teaching-learning process. Findings from these investigations have implications for the design of professional development and for engineering curricula. They highlight the importance of considering teacher beliefs about curriculum implementation and subject matter, as well as the importance of creating curriculum materials that focus teacher attention toward student thinking and the language rich science and engineering practices. Recommendations also include ongoing professional development to allow teachers time to try out and revise pedagogical routines that support the SEPs studied here.


    Directory of Open Access Journals (Sweden)

    Neta Dian Lestari


    Full Text Available Facing the business challenges of MEA Era is a challenge that should be faced by us, as an Educator I feel compelled to be able to participate in the success of my students, to face the challenge. The purpose of this study is not only for the Financial Statements of Bazaar, but also seek emphasis on the experience of an entrepreneurial process because through the Bazaar students are required to interact with people, how to offer well and politely to consumers, convince consumers to buy the products they offer and give explanation and the best service for consumers to be satisfied. Based on the result of observations which are conducted by the researcher as many as 15 times, SWOT observation analysis is with an average amount of 65.5% in GOOD Category. The results of financial statements analysis each group to see three important elements are: Elements Report L /R with the percentage of 56.09% High category, Elements of Capital Changes with the percentage of 78.05% Very High category, and Element Balance with the percentage of Current Assets 63.67%, High category, Fixed Assets 14.38% Low category and Liability 78.05% Very High category. This bazaar activity is very useful for students to gain experience and learning to become entrepreneurs. This activity is a very useful activity for students especially students of Accounting Education Studies Program FKIP University PGRI Palembang. By doing this activity, the students are expected to know how to offer and market a product that they make themselves well, and students can know more how to interact well and politely with consumers or customers.

  15. Integrating Engineering into Delaware's K-5 Classrooms: A Study of Pedagogical and Curricular Resources (United States)

    Grusenmeyer, Linda Huey


    This study examines the personal and curricular resources available to Delaware's elementary teachers during a time of innovative curriculum change, i.e., their knowledge, goals and beliefs regarding elementary engineering curriculum and the pedagogical support to teach two Science and Engineering Practices provided by science teaching materials.…

  16. Indigenous Education for Critical Democracy: Teacher Approaches and Learning Outcomes in a K-5 Indian Education for All Program (United States)

    Ngai, Phyllis B.; Koehn, Peter H.


    This article focuses on how three dimensions of critical democracy preparation (place-based geographical knowledge, social and political awareness of American Indian history and culture, and orientations conducive to the development of personal connections with American Indians) were impacted by different instructional approaches introduced when…

  17. Assessing K-5 Teacher Leaders' Mathematical Understanding: What Have the Test Makers and the Test Takers Learned? (United States)

    Ellington, Aimee J.; Whitenack, Joy W.; Inge, Vickie L.; Murray, Megan K.; Schneider, Patti J.


    This article describes the design and implementation of an assessment instrument for Numbers and Operations, the first course in a program to train elementary mathematics specialists. We briefly describe the course and its content, and then we elaborate on the process we used to develop the assessment instrument and the corresponding rubric for…

  18. The Effects of New Jersey's K-8 Foreign Language Authorization on K-5 Foreign Language Teaching: Two Teachers' Perspectives (United States)

    Raymond, Robert B. L.


    Recent research in language education policy (LEP) refocuses attention from the role of governments to local stakeholders that shape LEP. However, little attention has been given to teacher agency in LEP implementation for early foreign language (FL) education in the United States. This pilot study considers the role of foreign language elementary…

  19. Effects of vitamin K3 and K5 on proliferation, cytokine production, and regulatory T cell-frequency in human peripheral-blood mononuclear cells. (United States)

    Hatanaka, Hiroshige; Ishizawa, Hitomi; Nakamura, Yurie; Tadokoro, Hiroko; Tanaka, Sachiko; Onda, Kenji; Sugiyama, Kentaro; Hirano, Toshihiko


    The effects of vitamin K (VK) derivatives VK3 and VK5 on human immune cells have not been extensively investigated. We examined the effects of VK3 and VK5 on proliferation, apoptosis, cytokine production, and CD4+CD25+Foxp3+ regulatory T (Treg) cell-frequency in human peripheral blood mononuclear cells (PBMCs) activated by T cell mitogen in vitro. Anti-proliferative effects of VK3 and VK5 on T-cell mitogen activated PBMCs were assessed by WST assay procedures. Apoptotic cells were determined as Annexin V positive/propidium iodide (PI) negative cells. Cytokine concentrations in the supernatant of the culture medium were measured with bead-array procedures followed by analysis with flow cytometry. The CD4+CD25+Foxp3+Treg cells in mitogen-activated PBMCs were stained with fluorescence-labeled specific antibodies followed by flow cytometry. VK3 and VK5 suppressed the mitogen-activated proliferation of PBMCs significantly at 10-100μM (p<0.05). The data also suggest that VK3 and VK5 promote apoptosis in the mitogen-activated T cells. VK3 and VK5 significantly inhibited the production of tumor necrosis factor (TNF) α, interleukin (IL)-4, -6, and -10 from the activated PBMCs at 10-100μM (p<0.05). In contrast, VK3 and VK5 significantly increased Treg cell-frequency in the activated PBMCs at concentrations more than 10μM (p<0.001). Our data suggest that VK3 and VK5 attenuate T cell mediated immunity by inhibiting the proliferative response and inducing apoptosis in activated T cells. Copyright © 2014 Elsevier Inc. All rights reserved.

  20. Assessing Agricultural Literacy Elements of Project Food Land and People in K-5 Using the Food and Fiber Systems Literacy Standards (United States)

    Powell, David V.; Agnew, David M.


    Agricultural literacy has been evolving as a discipline for over 25 years. In agriculture, as other disciplines of education, the body of knowledge can be identified and measured by a set of standards. The Food and Fiber Systems Literacy Standards, developed in the 1990s, have been widely accepted as the standards for agricultural literacy. Also…

  1. Building the Foundation of Acceptance Book by Book: Lesbian, Gay, Bisexual, and/or Transgender-Themed Books for Grades K-5 Multicultural Libraries (United States)

    Knoblauch, Dee


    This article is an analysis of picture books and chapter books involving lesbian, gay, bisexual, and/or transgender characters. Over 30 picture and chapter books were reviewed, each of which would be appropriate for use in elementary classrooms. For grades K-2, several picture books highlight different types of families, including families that…

  2. How Are Teachers Integrating Technology in K-5 Classrooms? Studying Student Cognitive Engagement Using the Instructional Practices Inventory-Technology (IPI-T) Instrument (United States)

    Dennis, Larinee B.


    "It is often assumed that changing the classroom by introducing technology will result in better teaching and increased student motivation, which ultimately means more effective student learning experiences" (Donovan, Green, & Hartley, 2010, p. 423). But does it? This is the controversy and debate that surrounds the promise and…

  3. Three-dimensional poor man's Navier-Stokes equation: a discrete dynamical system exhibiting k(-5/3) inertial subrange energy scaling. (United States)

    McDonough, J M


    Outline of the derivation and mathematical and physical interpretations are presented for a discrete dynamical system known as the "poor man's Navier-Stokes equation." Numerical studies demonstrate that velocity fields produced by this dynamical system are similar to those seen in laboratory experiments and in detailed simulations, and they lead to scaling for the turbulence kinetic energy spectrum in accord with Kolmogorov K41 theory.

  4. Key comparison BIPM.RI(I)-K5 of the air kerma standards of the ININ, Mexico and the BIPM in 137Cs gamma radiation (United States)

    Kessler, C.; Burns, D. T.; Alvarez Romero, J. T.; De la Cruz Hernández, D.; Cabrera Vertti, M. R.; Tovar-Muñoz, V. M.


    A direct comparison of the standards for air kerma of the Instituto Nacional de Investigaciones Nucleares (ININ), Mexico, and of the Bureau International des Poids et Mesures (BIPM) was carried out in the 137Cs radiation beam of the BIPM in February 2015. The comparison result, evaluated as a ratio of the ININ and the BIPM standards for air kerma, is 1.0048 with a combined standard uncertainty of 2.0 × 10-3. The results are analysed and presented in terms of degrees of equivalence for entry in the BIPM key comparison database. Main text. To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database The final report has been peer-reviewed and approved for publication by the CCRI, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

  5. Minutes of the inaugaral meeting of the reference group in connection with the project titled: Kukimbia: The impact of environmental refugees in Southern Africa (K5/2768/2), held on 21 June 2017 at 10:00am

    CSIR Research Space (South Africa)

    Jacobs-Mata, Inga M


    Full Text Available 3.1 No software products are envisaged but a documentary will be developed as well as other knowledge dissemination tools and products. 4 CAPACITY BUILDING : PROGRESS REPORT 4.1 The PL requested an alteration of the proposal from 1 PhD and 3...

  6. Discussion of the question whether a local authority can claim to be affected in its planning competence by a permit issued for construction of a radwaste processing plant. High Administrative Court Lueneburg, judgement of 21.1.1993 - 7 K 5/90

    International Nuclear Information System (INIS)

    Czajka, D.


    A local authority has taken legal action against the first partial permit for the construction of a radwaste conditioning pilot plant at Gorleben, claiming to be affected in its planning competence by the fact that transport of spent fuel elements between the spent fuel storage facility and the pilot plant 2 km away would have to proceed on the rural district road. The action has been discussed. Appealable head notes: A local authority is not affected in its planning competence by a permit issued for construction of a facility for radwaste processing, although the operation of said facility may result in radwaste being transported by a road crossing the local authority's territory. (orig.) [de

  7. Phase formation in the K2MoO4-Lu2(MoO4)3-Hf(MoO4)2 system and the structural study of triple molybdate K5LuHf(MoO4)6

    International Nuclear Information System (INIS)

    Romanova, E.Yu.; Bazarov, B.G.; Tushinova, Yu.L.; Fedorov, K.N.; Bazarova, Zh.G.; Klevtsova, R.F.; Glinskaya, L.A.


    Interactions in the ternary system K 2 MoO 4 -Lu 2 (MoO 4 ) 3 -Hf(MoO 4 ) 2 have been studied by X-ray powder diffraction and differential thermal analysis. A new triple (potassium lutetium hafnium) molybdate with the 5 : 1 : 2 stoichiometry has been found. Monocrystals of this molybdate have been grown. Its X-ray diffraction structure has been refined (an X8 APEX automated diffractometer, MoK α radiation, 1960 F(hkl), R = 0.0166). The trigonal unit cell has the following parameters: a = 10.6536(1) A, c = 37.8434(8) A, V=3719.75(9) A, Z = 6, space group R3-bar c. The mixed 3D framework of the structure is built of Mo tetrahedra sharing corners with two independent (Lu,Hf)O 6 octahedra. Two sorts of potassium atoms occupy large framework voids [ru

  8. High Performance InAs/In0.53Ga0.23Al0.24As/InP Quantum Dot 1.55 um Tunnel Injection Laser

    KAUST Repository

    Bhowmick, Sishir


    The characteristics of 1.55 ? InAs self-organized quantum-dot lasers, grown on (001) InP substrates by molecular beam epitaxy, have been investigated. Modulation doping of the dots with holes and tunnel injection of electrons have been incorporated in the design of the active (gain) region of the laser heterostructure. Large values of To=227 K (5 °C ? T ?45 °C) and 100 K (45 °C ? T ? 75 °C) were derived from temperature dependent measurements of the light-current characteristics. The modal gain per dot layer is 14.5 cm -1 and the differential gain derived from both light-current and small-signal modulation measurements is 0.8}\\times 10-15 cm}2. The maximum measured 3 rm dB small-signal modulation bandwidth is 14.4 GHz and the gain compression factor is 5.4\\times 10-17 cm}2. The lasers are characterized by a chirp of 0.6 AA for a modulation frequency of 10 GHz and a near zero ?-parameter at the peak of the laser emission. These characteristics are amongst the best from any 1.55 ? edge-emitting semiconductor laser. © 1965-2012 IEEE.

  9. AcEST: DK953909 [AcEST

    Lifescience Database Archive (English)

    Full Text Available A3B212_ORYSJ Putative uncharacterized protein OS=Oryza... 49 1e-04 tr|A2Y2K5|A2Y2K5_ORYSI Putative uncharact...+ ++DP I +SKSA + Y + + Sbjct: 76 VEEENDTPSATKSIRDPPVITVSKSATYALYALTY 110 >tr|A2Y2K5|A2Y2K5_ORYSI Putative un

  10. AcEST: DK953371 [AcEST

    Lifescience Database Archive (English)

    Full Text Available ragment) OS=Brass... 36 1.7 tr|Q590K5|Q590K5_9ROSI Maturase K OS=Phragmotheca ecuador... 464 S+F + HGD Sbjct: 67 SLFVYLHGD 75 >tr|Q590K5|Q590K5_9ROSI Maturase K OS=Phragmotheca ecuador

  11. Comparison of intramuscular alfaxalone and ketamine combined with dexmedetomidine and butorphanol for castration in cats. (United States)

    Khenissi, Latifa; Nikolayenkova-Topie, Olga; Broussaud, Ségolène; Touzot-Jourde, Gwenola


    Objectives Cardiorespiratory parameters and anaesthesia quality in cats anaesthetised with either intramuscular (IM) alfaxalone or ketamine both combined with dexmedetomidine and butorphanol for castration were evaluated. Methods Thirty-two client-owned cats were randomly assigned to receive either alfaxalone (A; 3 mg/kg IM) or ketamine (K; 5 mg/kg IM), combined with dexmedetomidine (10 μg/kg) and butorphanol (0.2 mg/kg). Heart rate (HR), respiratory rate (RR) and rectal temperature (T°) were recorded prior to drug administration. Pulse rate (PR) and RR were recorded 10 (T 10 ) and 15 (T 15 ) mins after injection (T 0 ). Cardiorespiratory values (PR, RR, SPO 2 , blood pressure, P E 'CO 2 ) were recorded every 5 mins for the duration of the procedure. Pain at injection, intubation and recovery were evaluated with simple descriptive scores. Feasibility of anaesthesia was evaluated by the number of top-ups of anaesthetic needed. Cat attitude, ability to walk and presence of ataxia were assessed several times after extubation (T exmin ) and the time between injection and extubation recorded. Pain was assessed at T ex120 and T ex240 with the 4Avet-pain score. Results The RR was significantly lower in group K at T 10 (RR K = 28 ±13.35 breaths per minute [brpm], RR A = 43.24 ±7.04 brpm) and T 15 (RR K = 28 ±11.53 brpm vs RR A = 43 ±12.18 brpm). Time to extubation was significantly longer in group A (T A = 62 ±14.6 mins, T K = 45.13 ± 7.38 mins). Cats in group K needed more top-ups, were more ataxic at T ex120 , had a worse recovery score at T ex60 and were less willing to walk at T ex30 . Conclusions and relevance Cats receiving alfaxalone had a longer but better quality recovery. Cardiorespiratory parameters were stable and within clinically acceptable values following IM injection of either alfaxalone or ketamine in healthy cats. Intramuscular alfaxalone is a suitable alternative to ketamine for short procedures requiring anaesthesia when used in combination

  12. Development of Novel Vaccines and Therapeutics Using Plant-Based Expression Systems (United States)


    stored at -20 Extract defatted soy flour in PBS Q Ion- Reverse-phase Chromatography Hydrophobic Chromatography 20kD 10kD 25kD 50kD 37kD 5kD 20kD...using white flake, (the crude protein preparation remaining after oil removal and extraction with hexane) as the starting material. An overview of...clarified using a filter press to remove all the particulates. The clarified extract is subjected to anion- exchange chromatography on a Q

  13. Engineered Autologous Stromal Cells for the Delivery of Kringle 5, a Potent Endothelial Cell Specific Inhibitor for Anti-Angiogenic Breast Cancer Therapy

    National Research Council Canada - National Science Library

    Perri, Sabrina R


    .... To determine whether K5 possesses immune proinflammatory properties, we investigated the effects of K5 in an immune competent model of breast cancer and observed that tumor rejection is substantially...

  14. The Effects of Natural Weathering on Color Stability of Impregnated and Varnished Wood Materials

    Directory of Open Access Journals (Sweden)

    Turkay Turkoglu


    Full Text Available The aim of this study was to investigate effects of natural weathering on color stability of Scots pine (Pinus sylvestris L. and Oriental beech (Fagus orientalis L. impregnated with some chemicals [tanalith-E (TN-E, adolit-KD5 (AD-KD5, and chromated copper arsenate (CCA] and then varnished [synthetic varnish (SV and polyurethane varnish (PV]. While applying varnish increased lightness, impregnation decreased lightness of the wood specimens before natural weathering. Natural weathering caused greenish, bluish, and dark color tones of the wood surface. Total color change was increased with increasing exposure times in natural weathering. Untreated (control wood specimens exhibited higher color changes than the other wood specimens in all the stages of natural weathering. The total color changes of untreated Oriental beech specimens were less than untreated Scots pine specimens. The color stability of impregnated and varnished wood specimens gave better results than untreated and solely varnished wood specimens after natural weathering. The best color stability was obtained from both Oriental beech and Scots pine wood impregnated with TN-E before PV coating.

  15. Amelogenin interacts with cytokeratin-5 in ameloblasts during enamel growth. (United States)

    Ravindranath, Rajeswari M H; Basilrose, Rajam M; Ravindranath, Naren H; Vaitheesvaran, Bhavapriya


    The enamel protein amelogenin binds to GlcNAc (Ravindranath, R. M. H., Moradian-Oldak, R., and Fincham, A.G. (1999) J. Biol. Chem. 274, 2464-2471) and to the GlcNAc-mimicking peptide (GMp) (Ravindranath, R. M. H., Tam, W., Nguyen, P., and Fincham, A. G. (2000) J. Biol. Chem. 275, 39654-39661). The GMp motif in the N-terminal region of the cytokeratin 14 of ameloblasts binds to trityrosyl motif peptide (ATMP) of amelogenin (Ravindranath, R. M. H., Tam, W., Bringas, P., Santos, V., and Fincham, A. G. (2001) J. Biol. Chem. 276, 36586 - 36597). K14 (Type I) pairs with K5 (Type II) in basal epithelial cells; GlcNAc-acylated K5 is identified in ameloblasts. Dosimetric analysis showed the binding affinity of amelogenin to K5 and to GlcNAc-acylated-positive control, ovalbumin. The specific binding of [3H]ATMP with K5 or ovalbumin was confirmed by Scatchard analysis. [3H]ATMP failed to bind to K5 after removal of GlcNAc. Blocking K5 with ATMP abrogates the K5-amelogenin interaction. K5 failed to bind to ATMP when the third proline was substituted with threonine, as in some cases of human X-linked amelogenesis imperfecta or when tyrosyl residues were substituted with phenylalanine. Confocal laser scan microscopic observations on ameloblasts during postnatal (PN) growth of the teeth showed that the K5-amelogenin complex migrated from the cytoplasm to the periphery (on PN day 1) and accumulated at the apical region on day 3. Secretion of amelogenin commences from day 1. K5, similar to K14, may play a role of chaperone during secretion of amelogenin. Upon secretion of amelogenin, K5 pairs with K14. Pairing of K5 and K14 commences on day 3 and ends on day 9. The pairing of K5 and K14 marks the end of secretion of amelogenin.

  16. Characterization and visualization of cholecystokinin receptors in rat brain using [3H]pentagastrin

    International Nuclear Information System (INIS)

    Gaudreau, P.; Quirion, R.; St Pierre, S.; Pert, C.B.


    [ 3 H]Pentagastrin binds specifically to an apparent single class of CCK receptors on slide-mounted sections of rat brain (KD . 5.6 nM; Bmax . 36.6 fmol/mg protein). This specific binding is temperature-dependent and regulated by ions and nucleotides. The relative potencies of C-terminal fragments of CCK-8(SO 3 H), benzotript and proglumide in inhibiting specific [ 3 H]pentagastrin binding to CCK brain receptors reinforce the concept of different brain and pancreas CCK receptors. CCK receptors were visualized by using tritium-sensitive LKB film analyzed by computerized densitometry. CCK receptors are highly concentrated in the cortex, dentate gyrus, granular and external plexiform layers of the olfactory bulb, anterior olfactory nuclei, olfactory tubercle, claustrum, accumbens nucleus, some nuclei of the amygdala, thalamus and hypothalamus

  17. AcEST: DK954980 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 1|ENC_DROME Protein encore OS=Drosophila melanogaster GN... 53 2e-06 sp|Q9Y2K5|R3...DEVRQSPYLCPLSLDRKA-------KSFEEREEDYDRARSRIFS 573 >sp|Q9Y2K5|R3HD2_HUMAN R3H domain-containing protein 2 OS=H

  18. Kumulanty v profilové analýze

    Czech Academy of Sciences Publication Activity Database

    Čerňanský, Marian


    Roč. 15, 2a (2008), k45-k46 ISSN 1211-5894. [Regional conference on powder diffraction (17.) - RPDK. Valtice, 16.06.2008-20.06.2008] Institutional research plan: CEZ:AV0Z10100520 Keywords : cumulants * moments * diffraction profile * convolution Subject RIV: BM - Solid Matter Physics ; Magnetism

  19. Demonstration of intermediate cells during human prostate epithelial differentiation in situ and in vitro using triple-staining confocal scanning microscopy. (United States)

    van Leenders, G; Dijkman, H; Hulsbergen-van de Kaa, C; Ruiter, D; Schalken, J


    In human prostate epithelium, morphologically basal and luminal cells can be discriminated. The basal cell layer that putatively contains progenitor cells of the secretory epithelium is characterized by the expression of keratins (K) 5 and 14. Luminal cells represent the secretory compartment of the epithelium and express K8 and 18. We developed a technique for the simultaneous analysis of K5, 14, and 18 to identify intermediate cell stages in the prostate epithelium and to study the dynamic aspects of its differentiation in vitro. Nonmalignant prostate tissue and primary epithelial cultures were immunohistochemically characterized using triple staining with antibodies for K5, K14, and K18. Antibodies for K18 and K5 were conjugated directly with fluorochromes Alexa 488 and 546. K14 was visualized indirectly with streptavidin-Cy5. Keratin expression was analyzed by confocal scanning microscopy. The occurrence of exocrine and neuroendocrine differentiation in culture was determined via antibodies to prostate-specific antigen (PSA), chromogranin A, and serotonin. We found that basal cells expressed either K5(++)/14(++)/18+ or K5(++)/18+. The majority of luminal cells expressed K18(++), but colocalization of K5+/18(++) were recognized. Epithelial monolayer cultures predominantly revealed the basal cell phenotype K5(++)/14(++)/18+, whereas intermediate subpopulations expressing K5+/14+/18(++) and K5+/18(++) were also identified. On confluence, differentiation was induced as multicellular gland-like buds, and extensions became evident on top of the monolayer. These structures were composed of K18(++)- and K5+/18(+)-positive cell clusters surrounded by phenotypically basal cells. Few multicellular structures and cells in the monolayer showed exocrine differentiation (PSA+), but expression of chromogranin A and serotonin was absent. We conclude that simultaneous evaluation of keratin expression is useful for analyzing epithelial differentiation in the prostate. During this

  20. O-sulfated bacterial polysaccharides with low anticoagulant activity inhibit metastasis. (United States)

    Borgenström, Marjut; Wärri, Anni; Hiilesvuo, Katri; Käkönen, Rami; Käkönen, Sanna; Nissinen, Liisa; Pihlavisto, Marjo; Marjamäki, Anne; Vlodavsky, Israel; Naggi, Annamaria; Torri, Giangiacomo; Casu, Benito; Veromaa, Timo; Salmivirta, Markku; Elenius, Klaus


    Heparin-like polysaccharides possess the capacity to inhibit cancer cell proliferation, angiogenesis, heparanase-mediated cancer cell invasion, and cancer cell adhesion to vascular endothelia via adhesion receptors, such as selectins. The clinical applicability of the antitumor effect of such polysaccharides, however, is compromised by their anticoagulant activity. We have compared the potential of chemically O-sulfated and N,O-sulfated bacterial polysaccharide (capsular polysaccharide from E. COLI K5 [K5PS]) species to inhibit metastasis of mouse B16-BL6 melanoma cells and human MDA-MB-231 breast cancer cells in two in vivo models. We demonstrate that in both settings, O-sulfated K5PS was a potent inhibitor of metastasis. Reducing the molecular weight of the polysaccharide, however, resulted in lower antimetastatic capacity. Furthermore, we show that O-sulfated K5PS efficiently inhibited the invasion of B16-BL6 cells through Matrigel and also inhibited the in vitro activity of heparanase. Moreover, treatment with O-sulfated K5PS lowered the ability of B16-BL6 cells to adhere to endothelial cells, intercellular adhesion molecule-1, and P-selectin, but not to E-selectin. Importantly, O-sulfated K5PSs were largely devoid of anticoagulant activity. These findings indicate that O-sulfated K5PS polysaccharide should be considered as a potential antimetastatic agent.

  1. 76 FR 9706 - Finding of Substantial Inadequacy of Implementation Plan; Call for Iowa State Implementation Plan... (United States)


    ... advance and available for prompt implementation once triggered. Section 110(k)(5) of the CAA provides that... Environmental protection, Air pollution control, Iowa, Particulate matter, State Implementation Plan. Dated...

  2. Veterans Crisis Line: Videos About Reaching out for Help

    Medline Plus

    Full Text Available ... v/K5u3sb-Dbkc Watch additional videos about getting help. Behind the Scenes see more videos from Veterans Health Administration Be There: Help Save a Life see more videos from Veterans ...

  3. Mutant PIK3CA Induces EMT in a Cell Type Specific Manner.

    Directory of Open Access Journals (Sweden)

    Divya Bhagirath

    Full Text Available Breast cancer is characterized into different molecular subtypes, and each subtype is characterized by differential gene expression that are associated with distinct survival outcomes in patients. PIK3CA mutations are commonly associated with most breast cancer subtypes. More recently PIK3CA mutations have been shown to induce tumor heterogeneity and are associated with activation of EGFR-signaling and reduced relapse free survival in basal subtype of breast cancer. Thus, understanding what determines PIK3CA induced heterogeneity and oncogenesis, is an important area of investigation. In this study, we assessed the effect of mutant PIK3CA together with mutant Ras plus mutant p53 on oncogenic behavior of two distinct stem/progenitor breast cell lines, designated as K5+/K19- and K5+/K19+. Constructs were ectopically overexpressed in K5+/K19- and K5+/K19+ stem/progenitor cells, followed by various in-vitro and in-vivo analyses. Oncogene combination m-Ras/m-p53/m-PIK3CA efficiently transformed both K5+/K19- and K5+/K19+ cell lines in-vitro, as assessed by anchorage-independent soft agar colony formation assay. Significantly, while this oncogene combination induced a complete epithelial-to-mesenchymal transition (EMT in K5+/K19- cell line, mostly epithelial phenotype with minor EMT component was seen in K5+/K19+ cell line. However, both K5+/K19- and K5+/K19+ transformed cells exhibited increased invasion and migration abilities. Analyses of CD44 and CD24 expression showed both cell lines had tumor-initiating CD44+/CD24low cell population, however transformed K5+/K19- cells had more proportion of these cells. Significantly, both cell types exhibited in-vivo tumorigenesis, and maintained their EMT and epithelial nature in-vivo in mice tumors. Notably, while both cell types exhibited increase in tumor-initiating cell population, differential EMT phenotype was observed in these cell lines. These results suggest that EMT is a cell type dependent

  4. Preparation, characterization and photocatalytic studies of Cu , Sn ...

    Indian Academy of Sciences (India)

    method. Metal ions (Cu2+ and Sn2+), and non-metal anion, N3−, were substituted into the K5Sb5P2O20 for pos- ... in the photocatalytic reaction was studied using their appropriate scavengers. Keywords. ... K5Sb5P2O20 and exchange of its potassium with copper and tin ions ... distilled water and dried in air at 100. ◦. C.

  5. AcEST: DK962463 [AcEST

    Lifescience Database Archive (English)

    Full Text Available =Mus musc... 31 5.6 sp|Q9Y2K5|R3HD2_HUMAN R3H domain-containing protein 2 OS=Homo sa... 31 5.6 >sp|A0JNC2|R3...HSSCCNICCKKLCPLPHISAC 347 P+ SSCC CC+ C + S C Sbjct: 423 PQSQSSCCGACCESWCEIFRYSCC 446 >sp|Q9Y2K5|R3HD2_HUMAN

  6. Haploid genetic screens identify an essential role for PLP2 in the downregulation of novel plasma membrane targets by viral E3 ubiquitin ligases.

    Directory of Open Access Journals (Sweden)

    Richard T Timms

    Full Text Available The Kaposi's sarcoma-associated herpesvirus gene products K3 and K5 are viral ubiquitin E3 ligases which downregulate MHC-I and additional cell surface immunoreceptors. To identify novel cellular genes required for K5 function we performed a forward genetic screen in near-haploid human KBM7 cells. The screen identified proteolipid protein 2 (PLP2, a MARVEL domain protein of unknown function, as essential for K5 activity. Genetic loss of PLP2 traps the viral ligase in the endoplasmic reticulum, where it is unable to ubiquitinate and degrade its substrates. Subsequent analysis of the plasma membrane proteome of K5-expressing KBM7 cells in the presence and absence of PLP2 revealed a wide range of novel K5 targets, all of which required PLP2 for their K5-mediated downregulation. This work ascribes a critical function to PLP2 for viral ligase activity and underlines the power of non-lethal haploid genetic screens in human cells to identify the genes involved in pathogen manipulation of the host immune system.

  7. Binding of kappa- and sigma-opiates in rat brain

    International Nuclear Information System (INIS)

    Wolozin, B.L.; Nishimura, S.; Pasternak, G.W.


    Detailed displacements of [ 3 H]dihydromorphine by ketocyclazocine and SKF 10,047, [ 3 H]ethylketocyclazocine by SKF 10,047, and [ 3 H]SKF 10,047 by ketocyclazocine are all multiphasic, suggesting multiple binding sites. After treating brain tissue in vitro with naloxazone, all displacements lose the initial inhibition of 3 H-ligand binding by low concentrations of unlabeled drugs. Together with Scatchard analysis of saturation experiments, these studies suggest a common site which binds mu-, kappa, and sigma-opiates and enkephalins equally well and with highest affinity (KD less than 1 nM). The ability of unlabeled drugs to displace the low affinity binding of [ 3 H]dihydromorphine (KD . 3 nM), [ 3 H]ethylketocyclazocine (KD . 4 nM), [ 3 H]SKF 10,047 (KD . 6 nM), and D-Ala2-D-Leu5-[ 3 H]enkephalin (KD . 5 nM) remaining after treating tissue with naloxazone demonstrates unique pharmacological profiles for each. These results suggest the existence of distinct binding sites for kappa- and sigma-opiates which differ from those sites which selectively bind morphine (mu) and enkephalin

  8. Titration calorimetry of anesthetic-protein interaction: negative enthalpy of binding and anesthetic potency. (United States)

    Ueda, I; Yamanaka, M


    Anesthetic potency increases at lower temperatures. In contrast, the transfer enthalpy of volatile anesthetics from water to macromolecules is usually positive. The transfer decreases at lower temperature. It was proposed that a few selective proteins bind volatile anesthetics with negative delta H, and these proteins are involved in signal transduction. There has been no report on direct estimation of binding delta H of anesthetics to proteins. This study used isothermal titration calorimetry to analyze chloroform binding to bovine serum albumin. The calorimetrically measured delta H cal was -10.37 kJ.mol-1. Thus the negative delta H of anesthetic binding is not limited to signal transduction proteins. The binding was saturable following Fermi-Dirac statistics and is characterized by the Langmuir adsorption isotherms, which is interfacial. The high-affinity association constant, K, was 2150 +/- 132 M-1 (KD = 0.47 mM) with the maximum binding number, Bmax = 3.7 +/- 0.2. The low-affinity K was 189 +/- 3.8 M-1 (KD = 5.29 mM), with a Bmax of 13.2 +/- 0.3. Anesthetic potency is a function of the activity of anesthetic molecules, not the concentration. Because the sign of delta H determines the temperature dependence of distribution of anesthetic molecules, it is irrelevant to the temperature dependence of anesthetic potency.

  9. Application of a fluorescent cobalamin analogue for analysis of the binding kinetics. A study employing recombinant human transcobalamin and intrinsic factor

    DEFF Research Database (Denmark)

    Fedosov, Sergey N; Grissom, Charles B; Fedosova, Natalya U


    facilitated detailed kinetic analysis of Cbl binding. We found that TC had the same affinity for CBC and Cbl (K(d) = 5 x 10(-15) m), whereas interaction of CBC with the highly specific protein IF was more complex. For instance, CBC behaved normally in the partial reactions CBC + IF(30) and CBC + IF(20) when...... complexes. We suggest that the correct domain-domain interactions are the most important factor during recognition and fixation of the ligands by IF. Dissociation of IF-CBC was biphasic, and existence of multiple protein-analogue complexes with normal and partially corrupted structure may explain...... this behaviour. The most stable component had K(d) = 1.5 x 10(-13) m, which guarantees the binding of CBC to IF under physiological conditions. The specific intestinal receptor cubilin bound both IF-CBC and IF-Cbl with equal affinity. In conclusion, the fluorescent analogue CBC can be used as a reporting agent...

  10. Reduced number of alpha- and beta-adrenergic receptors in the myocardium of rats exposed to tobacco smoke

    Energy Technology Data Exchange (ETDEWEB)

    Larue, D.; Kato, G.


    The concentration of alpha- and beta-adrenergic receptors--as measured by specific (/sup 3/H)WB-4101 and (-)-(/sup 3/H)dihydroalprenolol binding--was diminished by 60% below control values in the hearts of rats exposed to tobacco smoke. These changes in receptor numbers took place almost immediately after tobacco smoke exposure and were rapidly reversible after termination of the exposure. The dissociation constant, KD, for (/sup 3/H)WB-4101 was identical in exposed (KD . 0.34 +/- 0.09 nM) and control (KD . 0.35 +/- 0.07 nM) hearts but was significantly different in the case of (-)-(3H)dihydroalprenolol binding (exposed, KD . 2.83 +/- 0.30 mM vs. control KD . 5.22 +/- 0.61 nM). For beta-receptor binding there was no significant difference between exposed and control animals in the Ki values for (-)-epinephrine, (-)-norepinephrine, (-)-alprenolol, (+/-)-propranolol or timolol. (-)-Isoproterenol, however, was found to bind with lower affinity in exposed compared with control hearts. For alpha-receptor binding there was no significant difference between control and 'smoked' animals in the Ki values for (-)-epinephrine, (-0)-norepinephrine or phentolamine. The decrease in alpha- and beta-adrenergic receptor concentration may be related to the phenomenon of receptor desensitization resulting from a release of catecholamines in rats exposed to tobacco smoke.

  11. Existence of solutions for quasilinear random impulsive neutral differential evolution equation

    Directory of Open Access Journals (Sweden)

    B. Radhakrishnan


    Full Text Available This paper deals with the existence of solutions for quasilinear random impulsive neutral functional differential evolution equation in Banach spaces and the results are derived by using the analytic semigroup theory, fractional powers of operators and the Schauder fixed point approach. An application is provided to illustrate the theory. Keywords: Quasilinear differential equation, Analytic semigroup, Random impulsive neutral differential equation, Fixed point theorem, 2010 Mathematics Subject Classification: 34A37, 47H10, 47H20, 34K40, 34K45, 35R12

  12. Ligand and proton exchange dynamics in recombinant human myoglobin mutants. (United States)

    Lambright, D G; Balasubramanian, S; Boxer, S G


    Site-specific mutants of human myoglobin have been prepared in which lysine 45 is replaced by arginine (K45R) and aspartate 60 by glutamate (D60E), in order to examine the influence of these residues and their interaction on the dynamics of the protein. These proteins were studied by a variety of methods, including one and two-dimensional proton nuclear magnetic resonance spectroscopy, exchange kinetics for the distal and proximal histidine NH protons as a function of pH in the met cyano forms, flash photolysis of the CO forms, and ligand replacement kinetics. The electronic absorption and proton nuclear magnetic resonance spectra of the CO forms of these proteins are virtually identical, indicating that the structure of the heme pocket is unaltered by these mutations. There are, however, substantial changes in the dynamics of both CO binding and proton exchange for the mutant K45R, whereas the mutant D60E exhibits behavior indistinguishable from the reference human myoglobin. K45R has a faster CO bimolecular recombination rate and slower CO off-rate relative to the reference. The kinetics for CO binding are independent of pH (6.5 to 10) as well as ionic strength (0 to 1 M-NaCl). The exchange rate for the distal histidine NH is substantially lower for K45R than the reference, whereas the proximal histidine NH exchange rate is unaltered. The exchange behavior of the human proteins is similar to that reported for a comparison of the exchange rates for myoglobins having lysine at position 45 with sperm whale myoglobin, which has arginine at this position. This indicates that the differences in exchange rates reflects largely the Lys----Arg substitution. The lack of a simple correlation for the CO kinetics with this substitution means that these are sensitive to other factors as well. Specific kinetic models, whereby substitution of arginine for lysine at position 45 can affect ligand binding dynamics, are outlined. These experiments demonstrate that a relatively

  13. Genetics of trehalose biosynthesis in desert-derived Aureobasidium melanogenum and role of trehalose in the adaptation of the yeast to extreme environments. (United States)

    Jiang, Hong; Liu, Guang-Lei; Chi, Zhe; Hu, Zhong; Chi, Zhen-Ming


    Melanin plays an important role in the stress adaptation of Aureobasidium melanogenum XJ5-1 isolated from the Taklimakan desert. A trehalose-6-phosphate synthase gene (TPS1 gene) was cloned from K5, characterized, and then deleted to determine the role of trehalose in the stress adaptation of the albino mutant K5. No stress response element and heat shock element were found in the promoter of the TPS1 gene. Deletion of the TPS1 gene in the albino mutant rendered a strain DT43 unable to synthesize any trehalose, but DT43 still could grow in glucose, suggesting that its hexokinase was insensitive to inhibition by trehalose-6-phosphate. Overexpression of the TPS1 gene enhanced trehalose biosynthesis in strain ET6. DT43 could not grow at 33 °C, whereas K5, ET6, and XJ5-1 could grow well at this temperature. Compared with K5 and ET6, DT43 was highly sensitive to heat shock treatment, high oxidation, and high desiccation, but all the three strains demonstrated the same sensitivity to UV light and high NaCl concentration. Therefore, trehalose played an important role in the adaptation of K5 to heat shock treatment, high oxidation, and high desiccation.

  14. Alpha-amylase inhibitor, CS-1036 binds to serum amylase in a concentration-dependent and saturable manner. (United States)

    Honda, Tomohiro; Kaneno-Urasaki, Yoko; Ito, Takashi; Kimura, Takako; Matsushima, Nobuko; Okabe, Hiromi; Yamasaki, Atsushi; Izumi, Takashi


    (2R,3R,4R)-4-hydroxy-2-(hydroxymethyl)pyrrolidin-3-yl 4-O-(6-deoxy-β-D-glucopyranosyl)-α-D-glucopyranoside (CS-1036), which is an α-amylase inhibitor, exhibited biphasic and sustained elimination with a long t1/2 (18.4-30.0 hours) in rats and monkeys, but exhibited a short t1/2 (3.7-7.9 hours) in humans. To clarify the species differences in the t1/2, the plasma protein binding of CS-1036 was evaluated by ultrafiltration. A concentration-dependent and saturable plasma protein binding of CS-1036 was observed in rats and monkeys with the dissociation rate constant (KD) of 8.95 and 27.2 nM, and maximal binding capacity (Bmax) of 52.8 and 22.1 nM, respectively. By the assessments of the recombinant amylase and immunoprecipitation, the major binding protein of CS-1036 in rats was identified as salivary amylase (KD 5.64 nM). CS-1036 also showed concentration-dependent and saturable binding to human salivary and pancreatic amylase, with similar binding affinity in rats. However, the protein binding of CS-1036 was constant in human plasma (≤10.2%) due to the lower serum amylase level compared with rats and monkeys. From the calculation of the unbound fraction (fu) in plasma based on in vitro KD and Bmax, the dose-dependent increase in fu after oral administration is speculated to lead to a dose-dependent increase in total body clearance and a high area under the curve/dose at lower doses, such as 0.3 mg/kg in rats.

  15. Physicochemical properties of soils in the sago palm (Metroxylon spp.) growing area of Surat Thani province Thailand (United States)

    Ruairuen, W.; Sparrow, E. B.; Fochesatto, G. J.


    Sago palm is one of the most important plants for sustainable agriculture and rural development in tropical swampy and peaty soils. Where no major crops can grow without drainage or soil improvement. It stores large quantities of starch which can be further processed into various basic raw materials for food, animal feed, industrial uses and alternative energy. This study aims to investigate the physicochemical properties of soil across the sago palm growing areas at Surat Thani province Thailand, where major of sago palms growth naturally exists. The soil samples from three districts Khiri Rat Nikhom (KR; 9 sampling sites), Kanchanadit (KD; 5 sampling sites), and Khian Sa (KS; 2 sampling sites) were studied and compared at 0-15 cm depth during March to June 2016. Observations indicated that the physicochemical properties of soil varied in each growing area. Soil bulk densities averages were lower in KD (0.52 g cm-3) than those in KR (0.58 g cm-3) and KS (0.57 g cm-3). Soil texture around KD and KS were dominated by silty loam. While in KR soil texture was dominated by sandy loam. The average soil conductivity in KS (5.68 mS m-1) was higher than KR (2.62 mS m-1) and KD (1.65 mS m-1). Furthermore, we found the sago palms grow well in a range of soil pH from 5.52 to 7.15, average soil pH: KS (6.8) and KD (6.96), while acid in KR (5.84). We also discuss the conservation activities to adequately protect sago palm, most of which are significantly threatened by habitat destruction and unsustainable harvesting.

  16. Pesticide leaching from two Swedish topsoils of contrasting texture amended with biochar (United States)

    Larsbo, Mats; Löfstrand, Elisabeth; de Veer, David van Alphen; Ulén, Barbro


    The use of biochar as a soil amendment has recently increased because of its potential for long-term soil carbon sequestration and its potential for improving soil fertility. The objective of this study was to quantify the effects of biochar soil incorporation on pesticide adsorption and leaching for two Swedish topsoils, one clay soil and one loam soil. We used the non-reactive tracer bromide and the pesticides sulfosulfuron, isoproturon, imidacloprid, propyzamid and pyraclostrobin, substances with different mobility in soil. Adsorption was studied in batch experiments and leaching was studied in experiments using soil columns (20 cm high, 20 cm diameter) where 0.01 kg kg- 1 dw biochar powder originating from wheat residues had been mixed into the top 10 cm. After solute application the columns were exposed to simulated rain three times with a weekly interval and concentrations were measured in the effluent water. The biochar treatment resulted in significantly larger adsorption distribution coefficients (Kd) for the moderately mobile pesticides isoproturon and imidacloprid for the clay soil and for imidacloprid only for the loam soil. Relative leaching of the pesticides ranged from 0.0035% of the applied mass for pyraclostrobin (average Kd = 360 cm3 g- 1) to 5.9% for sulfosulfuron (average Kd = 5.6 cm3 g- 1). There were no significant effects of the biochar amendment on pesticide concentrations in column effluents for the loam soil. For the clay soil concentrations were significantly reduced for isoproturon, imidacloprid and propyzamid while they were significantly increased for the non-mobile fungicide pyraclostrobin suggesting that the transport was facilitated by material originating from the biochar amendment.

  17. Heavy Hyperfragments produced by 800 MeV/c k in Nuclear Emulsions

    International Nuclear Information System (INIS)

    Marcial, P.


    A statistical and phenomenological survey of nearly 1200 heavy hyperfragments produced by interaction of 800 MeV/c K with the heavy nuclei of llford K 5 emulsion is presented. The emulsion was exposed A statistical and phenomenological survey of nearly 1200 heavy hyperfragments produced by interaction of 800 MeV/c K tilde with the heavy nuclei of llford K5 emulsion is presented. The emulsion was exposed in Berkeley. The variation of long list of parameters dealing with both the production and desintegration of the hyperfragments, with the size of the primary interaction is given. (Author)

  18. The locally connected compact metric spaces embeddable in the plane

    DEFF Research Database (Denmark)

    Thomassen, Carsten


    We prove that a 2-connected, locally connected, compact topological space M is homeomorphic to a subset of the 2-sphere if and only if M is metrizable and contains none of the Kuratowski graphs K-5 and K-3,K-3.......We prove that a 2-connected, locally connected, compact topological space M is homeomorphic to a subset of the 2-sphere if and only if M is metrizable and contains none of the Kuratowski graphs K-5 and K-3,K-3....

  19. Down-Regulation of the Na+-Coupled Phosphate Transporter NaPi-IIa by AMP-Activated Protein Kinase

    Directory of Open Access Journals (Sweden)

    Miribane Dërmaku-Sopjani


    Full Text Available Background/Aims: The Na+-coupled phosphate transporter NaPi-IIa is the main carrier accomplishing renal tubular phosphate reabsorption. It is driven by the electrochemical Na+ gradient across the apical cell membrane, which is maintained by Na+ extrusion across the basolateral cell membrane through the Na+/K+ ATPase. The operation of NaPi-IIa thus requires energy in order to avoid cellular Na+ accumulation and K+ loss with eventual decrease of cell membrane potential, Cl- entry and cell swelling. Upon energy depletion, early inhibition of Na+-coupled transport processes may delay cell swelling and thus foster cell survival. Energy depletion is sensed by the AMP-activated protein kinase (AMPK, a serine/threonine kinase stimulating several cellular mechanisms increasing energy production and limiting energy utilization. The present study explored whether AMPK influences the activity of NAPi-IIa. Methods: cRNA encoding NAPi-IIa was injected into Xenopus oocytes with or without additional expression of wild-type AMPK (AMPKα1-HA+AMPKβ1-Flag+AMPKγ1-HA, of inactive AMPKαK45R (AMPKα1K45R+AMPKβ1-Flag+AMPKγ1-HA or of constitutively active AMPKγR70Q (AMPKα1-HA+AMPKβ1-Flag+AMPKγ1R70Q. NaPi-IIa activity was estimated from phosphate-induced current in dual electrode voltage clamp experiments. Results: In NaPi-IIa-expressing, but not in water-injected Xenopus oocytes, the addition of phosphate (1 mM to the extracellular bath solution generated a current (Ip, which was significantly decreased by coexpression of wild-type AMPK and of AMPKγR70Q but not of AMPKαK45R. The phosphate-induced current in NaPi-IIa- and AMPK-expressing Xenopus ooocytes was significantly increased by AMPK inhibitor Compound C (20 µM. Kinetic analysis revealed that AMPK significantly decreased the maximal transport rate. Conclusion: The AMP-activated protein kinase AMPK is a powerful regulator of NaPi-IIa and thus of renal tubular phosphate transport.

  20. Development and Building of Radioactive Concrete Pads for calibration of the airborne and ground gamma-ray spectrometers, used in mineral exploration and hydrocarbons

    International Nuclear Information System (INIS)

    Carlos, Dionisio Uendro


    Eight transportable calibration pads were built in to be used as concentration standards for portable and airborne gamma-ray spectrometers calibrations. The pads construction procedure is described in full detail. The pads, with dimensions of 1 m x 1 m x 0,30 m and masses between 593 kg and 673 kg were made radioactive by the addition of different amounts of k-feldspar, caldasite and monazitic sand to the concrete masses. The potassium, uranium and thorium concentration vary significantly in the pads, reaching maximum values of 5,7% of K, 45,6 ppm eU and 137 ppm eTh. The distribution of the gamma radiation flux from the pads surfaces and the heterogeneity magnitudes of the radioactive elements concentration were experimentally established. An example of gamma-ray spectrometer calibration is presented. (author)

  1. An experimental study on the factors that affect fatigue crack growth retardation behavior in SM45C steel

    International Nuclear Information System (INIS)

    Kim, Seon Jin; Kim, Jong Hoon; Ahn, Seok Hwan


    Constant ΔK fatigue crack growth tests were performed applying an intermediate multiple overload for SM45C steel. The purpose of the present study is to investigate the effects of specimen thickness at various baseline stress intensity levels(ΔK b ), overload application position(a/W) and overload application frequency(OL Hz ) on fatigue crack growth retardation behavior. The principal results are summarized as follows. The amount of retardation for a given ΔK b level is increased with increasing the baseline stress intensity level in all specimen thickness. The normalized minimum crack growth rate is increased with increasing the specimen thickness, except for ΔK=45MPa√m. The retardation cycle is decreased with increasing the overload application position and increased with the overload application frequency

  2. The state of physics - 1987

    International Nuclear Information System (INIS)

    Bromley, D.A.


    The first part of a translation of the lecture read in 1987 by the resigning president of the IUPAP. The lecture presented an outline of the outstanding achievements of physics in 1985-1987. The first part deals with the non-existence of hidden parameters in quantum mechanics, with progress in elementary particle physics, and with achievements in nuclear and atomic physics. Among others, a group of ''superachievements'' is pointed out: the discovery of superconductivity at relatively high temperatures, the establishment of the theory of supersymmetry and of superstrings on the way to Grand Unification, the plans for the Superconducting Supercollider Accelerator. Further topics discussed are: the time inversion invariance, proton decay, double beta decay, symmetry in nuclei, collisions of ultrarelativistic heavy ions, positrons from heavy nuclei collisions, high precision measurements in atomic physics, and compressed states. (A.K.). 45 figs

  3. Reading the Rainbow: LGBTQ-Inclusive Literacy Instruction in the Elementary Classroom (United States)

    Ryan, Caitlin L.; Hermann-Wilmarth, Jill M.


    Drawing on examples of teaching from elementary school classrooms, this timely book for practitioners explains why LGBTQ-inclusive literacy instruction is possible, relevant, and necessary in grades K-5. The authors show how expanding the English language arts curriculum to include representations of LGBTQ people and themes will benefit all…

  4. Engaging Students through Image and Word (United States)

    Newland, Abby


    This article focuses on the connection between the visual arts and language arts with the many teaching and learning possibilities that may arise from an art curriculum infused with language arts. As a K-5 art specialist in a rural Georgia public school, the author feels passionately about the importance of interdisciplinary art education for…

  5. Evaluating Gifted Identification Practice: Aptitude Testing and Linguistically Diverse Learners (United States)

    Matthews, Michael S.; Kirsch, Lauri


    The authors examined individually administered IQ scores from an entire K-5 population (N = 432) of Limited English Proficient students referred for gifted program eligibility determination in a single large urban district in the southeastern United States. Of 8 IQ tests compared, only 1, the Stanford-Binet V, had scores appreciably lower than…

  6. Selective kainate receptor (GluK1) ligands structurally based upon1H-Cyclopentapyrimidin-2,4(1H,3H)-dione: synthesis, molecular modeling, and pharmacological and biostructural characterization

    DEFF Research Database (Denmark)

    Venskutonyte, Raminta; Butini, Stefania; Coccone, Salvatore Sanna


    The physiological function of kainate receptors (GluK1- GluK5) in the central nervous system is not fully understood yet. With the aim of developing potent and selective GluK1 ligands, we have synthesized a series of new thiophene-based GluK1 agonists (6a-c) and antagonists (7a-d). Pharmacologica...

  7. Personalized Thematic Kits (United States)

    Bontrager, Sharon


    Teaching Spanish at the K-5 level is a passion of mine, and the author would like to share some of the practical applications that she finds most rewarding and effective. She has found enthusiastic response to the creation of detailed language learning kits that are rooted in storytelling, but expanded to include home-made board games,…

  8. Suburban School Opens Elementary Campus in the Heart of Memphis: St. George's Independent School, Memphis, Tennessee (United States)

    Stewart, Sarah


    St. George's has nearly 1,150 students on three campuses: an elementary campus in Germantown and a middle/upper school campus in Collierville, both suburbs of Memphis, and a second elementary campus in Memphis. The Memphis campus serves 140 students in pre-K-5th grade. All Memphis campus students receive financial aid based on need, and…

  9. A Case Study of a Child with Dyslexia and Spatial-Temporal Gifts (United States)

    Cooper, Eileen E.; Ness, Maryann; Smith, Mary


    This case study details the history and K-5 school experience of a boy with dyslexia and spatial-temporal gifts. It describes assessment, evaluation, and identification procedures; the learning specialist's interventions and program; the critical role of the parent; and the services provided by the gifted program. Specific interventions are…

  10. 49 CFR Appendix to Part 10 - Exemptions (United States)


    ... who provided information to the Government under an express or, prior to September 27, 1975, an implied promise of confidentiality (5 U.S.C. 552a(k) (5) and (7)): 1. Investigative Records System..., eligibility, or qualifications of individuals for Federal civilian employment, contracts, access to classified...

  11. Technology in Education: Technology Integration into the School's Curriculum (United States)

    Culver, Bobby L., Jr.


    Integrating technology into the school's curriculum is a very contentious issue. However, it is an important issue that schools need to consider and assess. The purpose of this study was to examine the relationship between K-5th grade teachers' perceptions of proficiency of technology equipment, experience with technology in education, and…

  12. 77 FR 11022 - Approval and Promulgation of Implementation Plans; State of Alaska; Regional Haze State... (United States)


    ... protocol was developed. See Figure III.K.5-2 of the SIP submittal. Included Sources--Emission sources... Implementation Plan (SIP) revision, submitted by the State of Alaska on April 4, 2011, as meeting the.... Requirements for Regional Haze SIPs A. The CAA and the Regional Haze Rule B. Baseline, Natural Conditions, and...

  13. Stimulation of the N-methyl-D-aspartate receptor has a trophic effect on differentiating cerebellar granule cells

    DEFF Research Database (Denmark)

    Balázs, R; Hack, N; Jørgensen, Ole Steen


    N-methyl-D-aspartate (NMDA) supplementation of cerebellar cultures enriched in granule neurones (about 90%) prevented the extensive cell loss which occurs when cultivation takes place, in serum containing media, in the presence of 'low' K+ (5-15 mM). Estimation of tetanus toxin receptors and N-CA...

  14. Annals of African Surgery July 2011 07.12.2011.indd

    African Journals Online (AJOL)


    with respiration, tender with an obvious Pfannesteil scar. Bowel sounds were present. Plain abdominal x-rays showed dilated large bowel loops, inverted U sign, and paucity of gas in the rectum. Preoperative laboratory results showed Na+ 150mmol/l,. K+ 5.1mmol/l, HCO3- 25mmol/l, urea 63mmol/l and Cre-.


    Indian Academy of Sciences (India)

    tion - 6. How Architectural Features Affect. Building During Earthquakes? C VRMurty. 48 Turbulence and Dispersion. K 5 Gandhi. BOOK REVIEWS. 86 Algebraic Topology. Siddhartha Gadgil. Front Cover. - .. ..-.......... -. Back Cover. Two-dimensional vertical section through a turbulent plume. (Courtesy: G S Shat, CAOS, IISc.).

  16. 78 FR 39036 - Self-Regulatory Organizations; NYSE MKT LLC; Notice of Filing and Immediate Effectiveness of... (United States)


    ... applicable non-financial listing standard requirements, including, without limitation, the requirements of... financial statements for a full fiscal year commencing on a date after the date of filing with the...-K, including all required audited financial statements (the ``Reverse Merger Form 8-K'').\\5\\ In...

  17. Long-wavelength behaviour of charge-charge structure factor in insulating and metallic ground state of condensed hydrogen under pressure

    International Nuclear Information System (INIS)

    Amato, M.A.; March, N.H.


    Using the quantum Monte Carlo charge-charge structure factor S qq (k) it is argued that (i) in the metallic phase the small k expasion is non-analytic at order k 5 , reflecting plasmon dispersion, while in the insulating phase the non-analyticity occurs at O(k 3 ), due to van der Waals interaction between the protons. (author) [pt

  18. Learning about Plants with STEAM: In a Yearlong Unit on Plants, Students Use Art to Make Models of Their Subjects (United States)

    Kurson, Rebecca


    In this article Rebecca Kurson describes her school garden, now in its second year, as one that the lower school (preK-5) students plant and observe as often as possible. They call the garden an "outdoor classroom," and the younger students are particularly interested in how the plants grow. Kruson had lots of garden activities…

  19. Functional Validation of Heteromeric Kainate Receptor Models. (United States)

    Paramo, Teresa; Brown, Patricia M G E; Musgaard, Maria; Bowie, Derek; Biggin, Philip C


    Kainate receptors require the presence of external ions for gating. Most work thus far has been performed on homomeric GluK2 but, in vivo, kainate receptors are likely heterotetramers. Agonists bind to the ligand-binding domain (LBD) which is arranged as a dimer of dimers as exemplified in homomeric structures, but no high-resolution structure currently exists of heteromeric kainate receptors. In a full-length heterotetramer, the LBDs could potentially be arranged either as a GluK2 homomer alongside a GluK5 homomer or as two GluK2/K5 heterodimers. We have constructed models of the LBD dimers based on the GluK2 LBD crystal structures and investigated their stability with molecular dynamics simulations. We have then used the models to make predictions about the functional behavior of the full-length GluK2/K5 receptor, which we confirmed via electrophysiological recordings. A key prediction and observation is that lithium ions bind to the dimer interface of GluK2/K5 heteromers and slow their desensitization. Copyright © 2017 Biophysical Society. Published by Elsevier Inc. All rights reserved.

  20. determination of weibull parameters and analysis of wind power

    African Journals Online (AJOL)


    shape parameter (k) and the scale factor(c) were obtained to be 6.7 m/s and 4.3 m/s, 0.91 MW and 0.25 MW, K~ 5.4 and. 2.1, and c ... China, the forecast is not different as the report of the ..... Distribution for Wind Energy Analysis”, J. Wind Eng.

  1. Dream Box Learning. What Works Clearinghouse Intervention Report (United States)

    What Works Clearinghouse, 2013


    "DreamBox Learning" is a supplemental online mathematics program that provides adaptive instruction for students in grades K-5 and focuses on number and operations, place value, and number sense. The What Works Clearinghouse (WWC) identified one study of "DreamBox Learning" that both falls within the scope of the Elementary…

  2. Established Independent School Collaborates with Social Service Agency to Launch New School: Community Partnership School, Pennsylvania (United States)

    Stewart, Sarah


    Community Partnership School (CPS) serves 90 to 95 students annually in preK-5th grade. Of these, 100 percent are African American or multiracial, and all qualify for free or reduced-price lunch. Community Partnership School began as a collaboration between Germantown Academy, which had trouble recruiting low-income students to its suburban…

  3. Please, Don't Go! We've Only Just Begun: Voices of Non-Tenured Teachers in a Deprofessionalizing Career (United States)

    Gold, Jason H.


    This qualitative case study entails the voices of seven participants who are currently teaching in an elementary school setting (K-5), and an additional two participants, one who left student teaching prior to completion of her collegiate program and one who was released at the end of her fourth year of teaching. Albert Bandura's (1977)…

  4. Exploring Bilingual Teachers' Beliefs about Academic Language Development in Mathematics Teaching: Implications for Bilingual Teachers' Professional Development (United States)

    Canales-Vela, Viola


    The achievement of mathematics within Hispanic youth is of great concern across the nation. In order to improve student achievement in mathematics, the nature of a mathematics teacher's complex belief system must be understood (McGee & Wang, 2014). The purpose of this exploratory qualitative study is to investigate the K-5 bilingual teachers'…

  5. Experiment list: SRX150674 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available s=Leukemia Chronic Myelogenous 18469470,89.4,7.1,725 GSM935595: Harvard ChipSeq K562 BRF1 std source_name=K5...62 || biomaterial_provider=ATCC || lab=Harvard || lab description=Struhl - Harvard University || datatype=Ch

  6. Experiment list: SRX150569 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available s=Leukemia Chronic Myelogenous 51487836,63.2,7.7,861 GSM935490: Harvard ChipSeq K562 BRF2 std source_name=K5...62 || biomaterial_provider=ATCC || lab=Harvard || lab description=Struhl - Harvard University || datatype=Ch

  7. Evaluation of the MIND Research Institute's Spatial-Temporal Math (ST Math) Program in California (United States)

    Wendt, Staci; Rice, John; Nakamoto, Jonathan


    The MIND Research Institute contracted with the Evaluation Research Program at WestEd to conduct an independent assessment of mathematics outcomes in elementary school grades across California that were provided with the ST Math program. Spatial-Temporal (ST) Math is a game-based instructional software designed to boost K-5 and secondary-level…

  8. The fourth sound of Korotkoff in pregnancy: a myth

    NARCIS (Netherlands)

    Franx, A.; Evers, I. M.; van der Pant, K. A.; van der Post, J. A.; Bruinse, H. W.; Visser, G. H.


    To determine the detection rate and the reproducibility of the first (K1), the fourth (K4) and the fifth (K5) phases of the Korotkoff sounds in pregnant women. In 77 pregnant women receiving antenatal care in a tertiary referral centre, two observers took 231 simultaneous blood pressure measurements

  9. From the University to the Classroom: Prospective Elementary Mathematics Specialists' Pedagogical Shifts (United States)

    Myers, Kayla D.; Swars, Susan L.; Smith, Stephanie Z.


    This project focuses on the development of prospective Elementary Mathematics Specialists (EMSs) in a K-5 Mathematics Endorsement Program. Program courses emphasized elementary mathematics content and pedagogy while providing opportunities for participants to evidence their learning through classroom teaching practice, all in an attempt to…

  10. Who Is Doing the Engineering, the Student or the Teacher? The Development and Use of a Rubric to Categorize Level of Design for the Elementary Classroom (United States)

    Nadelson, Louis S.; Pfiester, Joshua; Callahan, Janet; Pyke, Patricia


    Science, technology, engineering, and mathematics (STEM) professional development for K-5 teachers often includes engineering design as a focus. Because engineering applications provide perspective to both teachers and their students in terms of how mathematic and scientific principles are employed to solve real-world problems (Baine, 2004; Roden,…

  11. Microscopic study of low-lying collective bands in 77Kr

    Indian Academy of Sciences (India)

    Extensive experimental studies of 77Kr have recently been performed by Sylvan et al [4] and Johnson et al [5] (see also refs [6,7]). These experimental studies have resulted in the identification of positive parity and negative parity collective bands up to very high spin. The ground state for 77Kr is based on K = 5/2+ with the.

  12. 75 FR 36659 - Proposed Collection; Comment Request; Brain Power! The NIDA Junior Scientist Program and the... (United States)


    ... of the Brain Power! Program's ability to: Increase students' knowledge about the biology of the brain... Request; Brain Power! The NIDA Junior Scientist Program and the Companion Program, Brain Power! Challenge.... Proposed Collection: Title: Brain Power! The NIDA Junior Scientist Program, for grades K-5, and the...

  13. The SOS Club: A Practical Peer Helper Program. (United States)

    Scarborough, Janna L.


    Describes a peer helper program developed for students in grades K-5. The program applies the concept that each student has something positive to offer the school and is responsible for providing that service. Discusses program goals and objectives, ways to gain support for the program, training, implementation, and evaluation. (RJM)

  14. Extraction of trivalent lanthanides and actinides by a synergistic mixture of thenoyltrifluoroacetone and a linear polyether

    International Nuclear Information System (INIS)

    Ensor, D.D.; Shah, A.H.


    Mixtures of a two component system, a linear polyether, 1,13-bis[8-quinolyl]-1,4,7,10,13-pentaoxatridecane, K-5, and thenoyltrifluoroacetone, HTTA, have been shown to exhibit synergistic character in the extraction of trivalent lanthanides and actinides. The effect of the addition of K-5 to the organic phase on the extractions of Ce(III), Eu(III), Tm(III), Am(III), Cm(III), Bk(III), and Cf(III) by HTTA in chloroform from 0.5M NaNO 3 at 25 0 C has been measured. These results indicate the extraction is enhanced by the formation of M(TTA) 3 K-5 adduct in the organic phase. The organic phase stability constants for the formation of these synergistic species have been calculated for all the metals studied. The magnitude of these organic phase stability constants for K-5 are similar to other common neutral donors. The order of stability does not follow the normal trend based on charge-to-radius ratio, but follows a pattern based on size, with Am(III) being the most stable

  15. 77 FR 46653 - Defense Logistics Agency Privacy Program (United States)


    ... information permits more informed decision-making by the Department when making required suitability.... 552a(k)(2) and (k)(5). (4) Reasons: (i) From 5 U.S.C. 552a(c)(3), as granting access to the accounting... witnesses or making witnesses reluctant to cooperate; and lead to suppression, alteration, or destruction of...

  16. Report of Accomplishments under the Airport Improvement Program. (United States)



  17. 76 FR 33814 - Proposed Collection; Comment Request for Regulation Project (United States)


    ... collection requirements related to Additional First Year Depreciation Deduction. DATES: Written comments... . SUPPLEMENTARY INFORMATION: Title: Additional First Year Depreciation Deduction. OMB Number: 1545-2207... year depreciation deduction. Section 401(b) of the TRUIRJCA amends Sec. 168(k) by adding Sec. 168(k)(5...

  18. Mobilities of Language and Literacy Ideologies: Dual Language Graduates' Bilingualism and Biliteracy (United States)

    Granados, Nadia R.


    Using qualitative methodology, this research examines how graduates of a K-5 dual language immersion program have experienced multiple and competing social, cultural, institutional, and political forces at play in complex processes that ultimately affect one's mobilities of language, literacy, and learning. These students have now grown into…

  19. Why Do They Stay? Elementary Teachers' Perceptions of Job Satisfaction and Retention (United States)

    Perrachione, Beverly A.; Rosser, Vicki J.; Petersen, George J.


    The purpose of this study was to identify intrinsic and extrinsic variables that influence teacher job satisfaction and retention. A survey was sent to 300 randomly selected Missouri public elementary school teachers in grades K-5 having 5 or more years of teaching experience. The results from 201 respondents suggest that three intrinsic…

  20. A Study of a Collaborative Instructional Project Informed by Systemic Functional Linguistic Theory: Report Writing in Elementary Grades (United States)

    Brisk, Maria Estela; Hodgson-Drysdale, Tracy; O'Connor, Cheryl


    This study examined the teaching of report writing in PreK-5 through the lens of systemic functional linguistics theory. Teachers were part of a university-public school collaboration that included professional development on teaching genres, text organization, and language features. Grounded in this knowledge, teachers explicitly taught report…

  1. Final report on the decontamination of the Curium Source Fabrication Facility

    International Nuclear Information System (INIS)

    Schaich, R.W.


    The Curium Source Fabrication Facility (CSFF) at Oak Ridge National Laboratory (ORNL) was decontaminated to acceptable contamination levels for maintenance activities, using standard decontamination techniques. Solid and liquid waste volumes were controlled to minimize discharges to the ORNL waste systems. This program required two years of decontamination effort at a total cost of approximately $700K. 5 references, 7 figures, 2 tables

  2. Inclusion bodies as potential vehicles for recombinant protein delivery into epithelial cells (United States)


    Background We present the potential of inclusion bodies (IBs) as a protein delivery method for polymeric filamentous proteins. We used as cell factory a strain of E. coli, a conventional host organism, and keratin 14 (K14) as an example of a complex protein. Keratins build the intermediate filament cytoskeleton of all epithelial cells. In order to build filaments, monomeric K14 needs first to dimerize with its binding partner (keratin 5, K5), which is then followed by heterodimer assembly into filaments. Results K14 IBs were electroporated into SW13 cells grown in culture together with a “reporter” plasmid containing EYFP labeled keratin 5 (K5) cDNA. As SW13 cells do not normally express keratins, and keratin filaments are built exclusively of keratin heterodimers (i.e. K5/K14), the short filamentous structures we obtained in this study can only be the result of: a) if both IBs and plasmid DNA are transfected simultaneously into the cell(s); b) once inside the cells, K14 protein is being released from IBs; c) released K14 is functional, able to form heterodimers with EYFP-K5. Conclusions Soluble IBs may be also developed for complex cytoskeletal proteins and used as nanoparticles for their delivery into epithelial cells. PMID:22624805

  3. Preventing and Responding to Bullying: An Elementary School's 4-Year Journey (United States)

    McCormac, Mary E.


    Bullying continues to be a pervasive problem in schools and requires a schoolwide approach. This article describes the action research process used to examine the impact of a 4-year, K-5 school bullying prevention and intervention. The school counselors collaborated with students, staff, and parents to implement the program, and collected and…

  4. On planarity of compact, locally connected, metric spaces

    DEFF Research Database (Denmark)

    Richter, R. Bruce; Rooney, Brendan; Thomassen, Carsten


    Independently, Claytor [Ann. Math. 35 (1934), 809–835] and Thomassen [Combinatorica 24 (2004), 699–718] proved that a 2-connected, compact, locally connected metric space is homeomorphic to a subset of the sphere if and only if it does not contain K 5 or K 3;3. The “thumbtack space” consisting of...

  5. CNG: a potential transport fuel

    International Nuclear Information System (INIS)



    Compressed Natural Gas (CNG) is an alternative transport fuel. Advantages of its use are briefly described. Infra structural requirements, if it is to be used in India are outlined. Applications of CNG as transport fuel for buses and trucks in India are discussed. (P.R.K.). 5 refs

  6. Requirements engineering for human activity systems

    CSIR Research Space (South Africa)

    Erasmus, J


    Full Text Available railway crossing system Measure of Effectiveness Min Max Priority Points Weight Rail-road safety, APAs 1 0 2 80 24 Investment cost, $k - RC 250k 150k 6 20 6 Sustainment cost, $k/20 years - RC 250k 100k 4 40 12 Investment cost, $k - RTA 20k 5k 9 1 0...

  7. Bulletin of Materials Science | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    ... independent reflections. The donors form a trimerized column, and the band structure calculated by the tight-binding approximation shows band insulator properties. The temperature dependent of the d.c. resistivity shows a semiconducting behaviour with room temperature resistivity along the -axis; ρ 290 K = 5.6 ohm ...

  8. 78 FR 69651 - Privacy Act of 1974; System of Records (United States)


    ... Dispute Resolution Act (ADR); DIA Instruction 5145.001, Conflict Management Program; DIA Manual 60-1... provided access to the information except to the extent that disclosure would reveal the identity of a.... 552a(k)(5), but only to the extent that such material would reveal the identity of a confidential...

  9. Multivariate cluster analysis of some major and trace elements ...

    African Journals Online (AJOL)


    The chemical composition of water infiltrating ... Major and trace metals determined in soil and water from a ... The soil samples were air-dried at 29°C in a dust-free place for ... compact 3K5 X-ray generator (Ital IS Structures, Italy) was used for.

  10. Fostering Student Sense Making in Elementary Science Learning Environments: Elementary Teachers' Use of Science Curriculum Materials to Promote Explanation Construction (United States)

    Zangori, Laura; Forbes, Cory T.; Biggers, Mandy


    While research has shown that elementary (K-5) students are capable of engaging in the scientific practice of explanation construction, commonly-used elementary science curriculum materials may not always afford them opportunities to do so. As a result, elementary teachers must often adapt their science curriculum materials to better support…

  11. Children Designing & Engineering: Contextual Learning Units in Primary Design and Technology (United States)

    Hutchinson, Patricia


    The Children Designing & Engineering (CD&E) Project at the College of New Jersey is a collaborative effort of the College's Center for Design and Technology and the New Jersey Chamber of Commerce. The Project, funded by the National Science Foundation (NSF), has been charged to develop instructional materials for grades K-5. The twelve…

  12. Exploring Categorical Body Mass Index Trajectories in Elementary School Children (United States)

    Moreno-Black, Geraldine; Boles, Shawn; Johnson-Shelton, Deb; Evers, Cody


    Background: Studies of body mass index (BMI) change have focused on understanding growth trajectories from childhood to adolescence and adolescence to adulthood, but few have explored BMI trajectories solely in elementary (grades K-5) school children. This report complements these studies by exploring changes in obesity status using analytic…

  13. Jet-Boundary and Plan-Form Corrections for Partial-Span Models with Reflection Plane, End Plate, or No End Plate in a Closed Circular Wind Tunnel (United States)


    complete-span models. Such models are used to--best- advantage to determine the aerodynamic characteristics of wings, flaps, lateral-control devices, and...d~mensfons w? Indes> . . ... ,. ., I . .’ . .,. . , 84295 #aOO . ~- 4 g— --% ii E~ [039 E. miiw%“” NATto )w. AWIWRY mwl—nEmkMEwrK5 . faKw+- WIW m&J. I

  14. 2. Home 3. Journals 4. Resonance–Journal of Science Education 5 ...

    Indian Academy of Sciences (India)

    Reciprocal Basis in n-Dimensions and other. Ramifications. V Balakrishnan. 27 The Normal Distribution. Some Roles of Normality. 5 Ramasubramanian. 38 Know Your ... A Victory for Machine over Man. K 5 R Anjaneyulu. BOOK REVIEWS. A Darwin Seledion. A Comprehensive Darwin Anthology. Shailesh Deshpande and ...

  15. Assessing the genetic diversity of five Tanzanian chicken ecotypes ...

    African Journals Online (AJOL)

    Charles Moses Lyimo


    Dec 21, 2013 ... Tanzanian human population (Swai et al., 2007; Lwelamira et al., 2008). .... Least square means of phenotypic measurements of ulna length, shank ... (2005) was applied from K = 1 to K = 5 to determine the optimal .... Genetic distance estimates between Tanzanian indigenous chicken populations was ...

  16. JCSC_128_4_663-670_SI.docx

    Indian Academy of Sciences (India)


    Preparation, characterization and photocatalytic studies of Cu2+, Sn2+ and N3- substituted K5Sb5P2O20. CH SUDHAKAR REDDYa, SREENU Ka, J R ... Table S1: Lattice parameters of all the samples. Table S2: The observed and ... CSPO, SSPO and KSPON. Figure S1. FT-IR spectra of KSPO, CSPO, SSPO and KSPON.

  17. Leveraging Current Initiatives to Bring Earth and Space Science into Elementary and Early Childhood Classrooms: NGSS in the Context of the Classroom Technology Push (United States)

    Pacheco-Guffrey, H. A.


    Classroom teachers face many challenges today such as new standards, the moving targets of high stakes tests and teacher evaluations, inconsistent/insufficient access to resources and evolving education policies. Science education in the K-5 context is even more complex. NGSS can be intimidating, especially to K-5 educators with little science background. High stakes science tests are slow to catch up with newly drafted state level science standards, leaving teachers unsure about what to change and when to implement updated standards. Amid all this change, many schools are also piloting new technology programs. Though exciting, tech initiatives can also be overwhelming to teachers who are already overburdened. A practical way to support teachers in science while remaining mindful of these stressors is to design and share resources that leverage other K-5 school initiatives. This is often done by integrating writing or math into science learning to meet Common Core requirements. This presentation will suggest a method for bringing Earth and space science learning into elementary / early childhood classrooms by utilizing the current push for tablet technology. The goal is to make science integration reasonable by linking it to technology programs that are in their early stages. The roles and uses of K-5 Earth and space science apps will be examined in this presentation. These apps will be linked to NGSS standards as well as to the science and engineering practices. To complement the app resources, two support frameworks will also be shared. They are designed to help educators consider new technologies in the context of their own classrooms and lessons. The SAMR Model (Puentadura, 2012) is a conceptual framework that helps teachers think critically about the means and purposes of integrating technology into existing lessons. A practical framework created by the author will also be shared. It is designed to help teachers identify and address the important logistical

  18. Posterolateral Corner Reconstruction using the Anatomical Two-Tailed Graft Technique: Clinical Outcomes in the Multiligament Injured Knee. (United States)

    Woodmass, Jarret M; Sanders, Thomas L; Johnson, Nick R; Wu, Isabella T; Krych, Aaron J; Stuart, Michael J; Levy, Bruce A


    Injury to the posterolateral corner (PLC) of the knee can lead to both varus and rotational instability. Multiple PLC reconstruction techniques have been described, including one-tailed graft (fibula-based constructs) or two-tailed graft (combined fibula- and tibia-based constructs). The purpose of our study was to evaluate the clinical outcomes of anatomical two-tailed graft reconstruction of the PLC in the setting of multiligament knee injuries (MKLIs) with grade III varus instability. Patients were identified through a prospective MLKI database between 2004 and 2013. Patients who received fibular collateral ligament and PLC reconstructions using a two-tailed graft and had a minimum follow-up of 2 years were included. Patients were assessed for clinical laxity grade, range of motion, and functional outcomes using Lysholm and International Knee Documentation Committee (IKDC) scores. Twenty patients (16 male, 4 female) with a mean age of 30.7 (range: 16-52) and a mean follow-up of 52.2 months (range: 24-93 months) were included. Knee dislocation (KD) grades included: 4 KD-1, 10 KD 3-L, 5 KD-4, and 1 KD-5. No patients had isolated PLC injuries. Mean IKDC and Lysholm score were 73.1 ± 25.8 and 78 ± 26, respectively. Mean range of motion was -1.1 to 122.8. In full extension, two patients (10%) had grade 1 laxity to varus stress. In 30 degrees of knee flexion, five (25%) patients had grade 1 laxity, and two (10%) had grade 2 laxity. Anatomical two-tailed PLC reconstruction can reliably restore varus stability when performed on patients with MLKIs and type C posterolateral instability with hyperextension external rotation recurvatum deformity. Satisfactory functional outcome scores were achieved in the majority of patients. This study supports the use of an anatomical two-tailed PLC reconstruction in the multiligament injured knee. The level of evidence is IV, case series. Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

  19. Strengthening STEM Education through Community Partnerships. (United States)

    Lopez, Colleen A; Rocha, Jon; Chapman, Matthew; Rocha, Kathleen; Wallace, Stephanie; Baum, Steven; Lawler, Brian R; Mothé, Bianca R


    California State University San Marcos (CSUSM) and San Marcos Elementary Schools have established a partnership to offer a large-scale community service learning opportunity to enrich science curriculum for K-5 students. It provides an opportunity for science, technology, engineering, and math (STEM) majors to give back to the community, allowing them to experience teaching in an elementary classroom setting, in schools that lack the resources and science instructor specialization needed to instill consistent science curricula. CSUSM responded to this need for more STEM education by mobilizing its large STEM student body to design hands-on, interactive science lessons based on Next Generation Science Standards (NGSS). Since 2012, the program has reached out to over four thousand K-5 students, and assessment data have indicated an increase in STEM academic performance and interest.

  20. AcEST: DK944768 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 96 9e-19 tr|Q6AZL1|Q6AZL1_XENTR Finkel-Biskis-Reilly murine sarcoma virus... 96 1e-18 tr|B4MLP6|B4MLP6_DROW... musculus GN... 95 2e-18 tr|Q642K5|Q642K5_MOUSE Finkel-Biskis-Reilly murine sarcoma virus... 95 2e-18 tr|Q5BJN7|Q5BJN7_RAT Fink... tr|Q3SZG2|Q3SZG2_BOVIN Finkel-Biskis-Reilly murine sarcoma virus... 95 2e-18 tr|Q4PM00|Q4PM00_IXOSC 40S rib...e-18 tr|Q56JV7|Q56JV7_BOVIN Ubiquitin-like/S30 ribosomal fusion prote... 95 2e-18

  1. AcEST: DK961406 [AcEST

    Lifescience Database Archive (English)

    Full Text Available is GN=MGC... 96 1e-18 tr|Q4KLF0|Q4KLF0_XENLA MGC116435 protein OS=Xenopus laevis GN=MG... 96 1e-18 tr|Q6AZL1|Q6AZL1_XENTR Fink...8 tr|Q91V99|Q91V99_MOUSE Fau protein (Fragment) OS=Mus musculus GN... 95 3e-18 tr|Q642K5|Q642K5_MOUSE Finkel...-Biskis-Reilly murine sarcoma virus... 95 3e-18 tr|Q5BJN7|Q5BJN7_RAT Finkel-Biskis-Reilly murine sarcoma vir...... 95 3e-18 tr|Q56JV7|Q56JV7_BOVIN Ubiquitin-like/S30 ribosomal fusion prote... 95 3e-18 tr|Q3SZG2|Q3SZG2_BOVIN Fink

  2. AcEST: DK947256 [AcEST

    Lifescience Database Archive (English)

    Full Text Available s GN=MG... 96 1e-18 tr|Q6AZL1|Q6AZL1_XENTR Finkel-Biskis-Reilly murine sarcoma virus... 96 1e-18 tr|B4MLP6|B...nt) OS=Mus musculus GN... 95 2e-18 tr|Q642K5|Q642K5_MOUSE Finkel-Biskis-Reilly murine sarcoma virus... 95 2e...-18 tr|Q5BJN7|Q5BJN7_RAT Finkel-Biskis-Reilly murine sarcoma virus (... 95 2e-18 .... 95 2e-18 tr|Q3SZG2|Q3SZG2_BOVIN Finkel-Biskis-Reilly murine sarcoma virus... 95 2e-18 tr|Q4PM00|Q4PM00_IXO

  3. AcEST: DK948905 [AcEST

    Lifescience Database Archive (English)

    Full Text Available MGC80045 protein OS=Xenopus laevis GN=MGC... 95 2e-18 tr|Q6AZL1|Q6AZL1_XENTR Finkel-Biskis-Reilly murine sar...t) OS=Mus musculus GN... 95 2e-18 tr|Q642K5|Q642K5_MOUSE Finkel-Biskis-Reilly murine sarcoma virus... 95 2e-...-like/S30 ribosomal fusion prote... 95 2e-18 tr|Q3SZG2|Q3SZG2_BOVIN Finkel-Biskis-Reilly murine sarcoma viru...18 tr|Q5BJN7|Q5BJN7_RAT Finkel-Biskis-Reilly murine sarcoma virus (... 95 2e-18 t

  4. AcEST: DK957349 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 96 9e-19 tr|Q6AZL1|Q6AZL1_XENTR Finkel-Biskis-Reilly murine sarcoma virus... 96 1e-18 tr|B4MLP6|B4MLP6_DROW... musculus GN... 95 2e-18 tr|Q642K5|Q642K5_MOUSE Finkel-Biskis-Reilly murine sarcoma virus... 95 2e-18 tr|Q5BJN7|Q5BJN7_RAT Fink... tr|Q3SZG2|Q3SZG2_BOVIN Finkel-Biskis-Reilly murine sarcoma virus... 95 2e-18 tr|Q4PM00|Q4PM00_IXOSC 40S rib...e-18 tr|Q56JV7|Q56JV7_BOVIN Ubiquitin-like/S30 ribosomal fusion prote... 95 2e-18

  5. AcEST: DK959327 [AcEST

    Lifescience Database Archive (English)

    Full Text Available F0_XENLA MGC116435 protein OS=Xenopus laevis GN=MG... 96 1e-18 tr|Q6AZL1|Q6AZL1_XENTR Finkel-Biskis-Reilly m... (Fragment) OS=Mus musculus GN... 95 3e-18 tr|Q642K5|Q642K5_MOUSE Finkel-Biskis-Reilly murine sarcoma virus...... 95 3e-18 tr|Q5BJN7|Q5BJN7_RAT Finkel-Biskis-Reilly murine sarcoma virus (... 95 3e-18 tr|Q3THB2|Q3THB2_MO...Ubiquitin-like/S30 ribosomal fusion prote... 95 3e-18 tr|Q3SZG2|Q3SZG2_BOVIN Finkel-Biskis-Reilly murine sar

  6. Effect of axial heat flux distribution on CHF

    Energy Technology Data Exchange (ETDEWEB)

    Park, Cheol


    Previous investigations for the effect of axial heat flux distributions on CHF and the prediction methods are reviewed and summarized. A total of 856 CHF data in a tube with a non-uniform axial heat flux distribution has been compiled from the articles and analyzed using the 1995 Groeneveld look-up table. The results showed that two representative correction factors, K5 of the look-up table and Tongs F factor, can be applied to describe the axial heat flux distribution effect on CHF. However, they overpredict slightly the measured CHF, depending on the quality and flux peak shape. Hence, a corrected K5 factor, which accounts for the axial heat flux distribution effect is suggested to correct these trends. It predicted the CHF power for the compiled data with an average error of 1.5% and a standard deviation of 10.3%, and also provides a reasonable prediction of CHF locations.

  7. Configurations and level structure of 219Rn

    International Nuclear Information System (INIS)

    Sheline, R.K.; Liang, C.F.; Paris, P.


    The level structure of 219 Rn has been studied using the alpha decay of 223 Ra and coincident gamma rays. While only modest changes are required in the level structure, and only above 342.8 keV, severe changes are required throughout the level scheme in the spin assigments. These changes allow the assignment of two sets of anomalous bands with K=5/2 ± and K=3/2 ± . The K=5/2 ± bands have configurations intermediate between the reflection asymmetric configuration and the g 9/2 shell model configuration, while the K=3/2 ± bands have configurations intermediate between the mixed reflection asymmetric configuration and the i 11/2 shell model configuration. Comparison of the systematics of 219 Rn with neighboring isotones, isobars, and isotopes shows clearly the collapse of the quadrupole-octupole-type configurations into the less degenerate shell model configurations. copyright 1998 The American Physical Society

  8. p63 protein is essential for the embryonic development of vibrissae and teeth

    International Nuclear Information System (INIS)

    Rufini, Alessandro; Weil, Miguel; McKeon, Frank; Barlattani, Alberto; Melino, Gerry; Candi, Eleonora


    Development of skin appendages strongly depends on epithelial-mesenchymal interactions. One of the genes involved in this process is p63, a member of the p53 family of transcription factors, essential for ectodermal development, as elucidated by the phenotype of p63 knock-out mice. Surprisingly, no information on p63 expression in tooth and hair is yet available. Here, we show p63 expression during teeth and vibrissae morphogenesis in mouse embryos and we also show a correlation with the expression patterns of the epithelial marker keratin 5 and the proliferation marker Ki67. Our results show that p63 colocalizes with both K5 and Ki67 in the epithelium of developing vibrissae, while in teeth p63 is expressed, together with K5, in the undifferentiated ectoderm (enamel organ), and in ameloblasts, a subpopulation of differentiated ectodermal cells. Moreover, p63 expression in tooth seems not to be fully colocalized with nuclear Ki67 expression

  9. AcEST: DK960773 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 0.026 tr|Q8K1K8|Q8K1K8_MOUSE Budding uninhibited by benzimidazoles 1 h... 37 0.046 tr|A2APR8|A2APR8_MOUSE Budding uninhibited by benz...VLYAD 116 >tr|Q53QE4|Q53QE4_HUMAN Putative uncharacterized protein BUB1 (BUB1 budding uninhibited by benzimidazoles...QGELQHASAVLQRGIQNQA 130 >tr|A8K5A6|A8K5A6_HUMAN cDNA FLJ76827, highly similar to Homo sapiens BUB1 budding uninhibited by benzimidazo...imidazoles 1 h... 37 0.046 tr|A6RAA5|A6RAA5_AJECN Putati...les 1 homolog (yeast) (BUB1), mRNA OS=Homo sapiens PE=2 SV=1 Length = 1085 Score =

  10. Thermo-Physical Properties of Kenaf-Filled Acrylonitrile Butadiene Styrene Composites

    Directory of Open Access Journals (Sweden)

    Nikmatin Siti


    Full Text Available Studies on advantageous of natural fillers incorporated into polymer composites on thermo-physical and mechanical properties are still intensively investigated. Several evidences suggest that the natural fillers with small contents combined with polymer increase their composite properties. We thus investigate thermo-physical properties of kenaf-filled acrylonitrile butadiene styrene (ABS composites. ABS with 5% kenaf microparticle size (ABS/K5, ABS with 5% kenaf short fiber (ABS/KSF5, and recycled ABS with 5% kenaf microparticle size (RABS/K5 were manufactured. Granular composites were manufactured by the twin screw extruder. Composite properties in terms of X-ray diffractions, surface morphologies, and thermal behaviors were investigated. The present work found that ABS/KSF5 has the highest degree of crystallinity compared to others. No significant difference was found in terms of thermal properties of the composites.

  11. Optimasi Lahan Dengan Sistem Tumpangsari Jagung Manis (Zea Mays Saccharata, Strurt) Dan Kangkung Sutera (Ipomea Reptans) Di Pekanbaru


    Surtinah, Surtinah; Susi, Neng; Lestari, Sri Utami


    Penelitian dilaksanakan secara eksperimen dengan menggunakan rancangan lingkungan acak lengkap dengan 3 kali ulangan, dan rancangan perlakuan yaitu waktu tanam kangkung sebagai tanaman tumpangsari pada budidaya jagung manis ( K ) yang terdiri dari : k1 = waktu tanam bersamaan; k2 = waktu tanam kangkung 1 minggu setelah tanam jagung manis; k3 = waktu tanam kangkung 2 minggu setelah tanam jagung manis; k4 = waktu tanam kangkung 3 minggu setelah tanam jagung manis; k5= penanaman jagung manis tu...

  12. Study of the Issues of Computational Aerothermodynamics Using a Riemann Solver (United States)


    should satisfy positiv - ity and not violate the Second Law of Thermodynamics (commonly called the entropy condition). Positivity preserving schemes...can be seen that N2 starts to dissociate around 6000 K - 12,000 K. 5.3 Conclusion This work has produced quantitative dividing lines between a regime...This figure provides a great deal of quantitative information and should be very useful to the high-speed flow CFD community. This work has also

  13. Uncovering the Hidden Molecular Signatures of Breast Cancer (United States)


    et al. (2010) Structure, evolutionary conservation, and conformational dynamics of Homo sapiens fascin-1, an F-actin crosslinking protein. J Mol Biol...Adenocarcinoma Adenocarcinoma C K8 /1 8 C K1 4 C K5 /6 Vi m en tin E- ca dh er in Sp in dl oi d tu m or c el ls At yp ic al d uc ts At yp ic al d

  14. Ectoderm-targeted overexpression of the glucocorticoid receptor induces hypohidrotic ectodermal dysplasia. (United States)

    Cascallana, Jose Luis; Bravo, Ana; Donet, Eva; Leis, Hugo; Lara, Maria Fernanda; Paramio, Jesús M; Jorcano, José L; Pérez, Paloma


    Hypohidrotic ectodermal dysplasia is a human syndrome defined by maldevelopment of one or more ectodermal-derived tissues, including the epidermis and cutaneous appendices, teeth, and exocrine glands. The molecular bases of this pathology converge in a dysfunction of the transcription factor nuclear factor of the kappa-enhancer in B cells (NF-kappaB), which is essential to epithelial homeostasis and development. A number of mouse models bearing disruptions in NF-kappaB signaling have been reported to manifest defects in ectodermal derivatives. In ectoderm-targeted transgenic mice overexpressing the glucocorticoid receptor (GR) [keratin 5 (K5)-GR mice], the NF-kappaB activity is greatly decreased due to functional antagonism between GR and NF-kappaB. Here, we report that K5-GR mice exhibit multiple epithelial defects in hair follicle, tooth, and palate development. Additionally, these mice lack Meibomian glands and display underdeveloped sweat and preputial glands. These phenotypic features appear to be mediated specifically by ligand-activated GR because the synthetic analog dexamethasone induced similar defects in epithelial morphogenesis, including odontogenesis, in wild-type mice. We have focused on tooth development in K5-GR mice and found that an inhibitor of steroid synthesis partially reversed the abnormal phenotype. Immunostaining revealed reduced expression of the inhibitor of kappaB kinase subunits, IKKalpha and IKKgamma, and diminished p65 protein levels in K5-GR embryonic tooth, resulting in a significantly reduced kappaB-binding activity. Remarkably, altered NF-kappaB activity elicited by GR overexpression correlated with a dramatic decrease in the protein levels of DeltaNp63 in tooth epithelia without affecting Akt, BMP4, or Foxo3a. Given that many of the 170 clinically distinct ectodermal dysplasia syndromes still remain without cognate genes, deciphering the molecular mechanisms of this mouse model with epithelial NF-kappaB and p63 dysfunction may

  15. An Evaluation of the Getz - Roanoke County School Division's School Counselor Peer Group Clinical Supervision Program


    Agnew, David T.


    (G-PGCS) was designed and implemented for K-6 school counselors. G-PGCS began in the fall of 1994 and has continued to the present; however, there have been no studies on the effects of the program. Therefore, the purpose of this study was to conduct a qualitative evaluation of G-PGCS. The evaluation participants included current Roanoke County K-5 school counselors, and selected administrators. The sources of data for the evalu...

  16. Characterization of nutrient disorders of gerbera hybrid 'Festival Light Eye Pink' (United States)

    Gerbera hybrid ‘Festival Yellow with Light Eye’ plants were grown in silica sand culture to induce and photograph nutritional disorder symptoms. Plants were grown with a complete modified Hoagland's all nitrate solution: (macronutrients in mM) 15 NO3-N, 1.0 PO4-P, 6.0 K, 5.0 Ca, 2.0 Mg, and 2.0 SO4...

  17. Accurate and efficient computation of synchrotron radiation functions

    International Nuclear Information System (INIS)

    MacLeod, Allan J.


    We consider the computation of three functions which appear in the theory of synchrotron radiation. These are F(x)=x∫x∞K 5/3 (y) dy))F p (x)=xK 2/3 (x) and G p (x)=x 1/3 K 1/3 (x), where K ν denotes a modified Bessel function. Chebyshev series coefficients are given which enable the functions to be computed with an accuracy of up to 15 sig. figures

  18. Design Evolution of a Fighter Training Scheduling Decision Support System. (United States)


    student class, date and time of the academic meeting, and the instructor as- signed to teach . Injuts/Dutieg-Meetingn. This screen must display the numerous...34Class Timeline* from the MAIN MENU. Allmgor.. Aids: The class names art depicted along the left side of the graph. Coto Mechanizmma: The EFl ...drives are inside the computer as actual "hard’ware, hence the name. K-5 Instructor Pilo (1k) An Instructor Pilot is a pilot that is qualified to teach

  19. The ionisation balance of C0 to C+4

    International Nuclear Information System (INIS)

    Nussbaumer, H.; Storey, P.J.


    The ionisation balance for the ions C 0 -C +4 has been calculated for 10 8 -3 ] 12 and 2 x 10 4 K 5 K. The presence of metastable terms is included in the calculation of the collisional dielectronic recombination and ionisation coefficients. The influence of the observed solar radiation field on the ionisation balance is investigated. Changes in that field do strongly influence the results. (orig.) [de

  20. Infrared Multispectral Sensor Program, Phase 2. Field Measurements, Analysis and Modeling. Volume 1. Fourier Transform Spectrometer Sensor Characterization. (United States)


    TskY=250K) ... 5-27 6-1. Treeline Correlation With 10.1 Microns ...................... 6-2 6-2. Mean Contrast: CARC Panel vs. Treeline ...6-3 6-3. CARC Panel and Treeline .............................. 6-5 6-4. Signal-to-Clutter Ratio for CARC Panel vs. Treeline ............. 6-6 6...5. Low Emissivity Panel and Treeline ......................... 6-7 xii TABLES 4-1: Sensor Characterization Test Summary ....................... 4-2 4

  1. Infrared absorption and Raman scattering spectroscopic studies of condensed ions

    International Nuclear Information System (INIS)

    Dao, N.Q.; Knidiri, M.


    Infrared and Raman spectra of the complex K 5 (UO 2 ) 2 F 9 were recorded in the region 4000 to 80 cm -1 . Factor group analysis was used to classify the internal vibrations of the binuclear ion (UO 2 ) 2 F 9 5- . Infrared and Raman spectra were assigned and splitting of the internal modes of the (UO 2 ) 2 F 9 5- anion interpreted. (author)

  2. The Usefulness of Impact Dampers for Space Applications. (United States)


    5 ’DTIC ditfibutif’n is uni td . 14 1983 THDEPARTMENT OF THE AIR FORCER ’.’. AIR UNIVERSITY (ATC)FAIR FORCE INSTITUTE OF TECHNOLOGY TilES I W right...Laboratory with Important Parameters 88 -. . . . . . - 4.. X0 a >1 0 t 0.. 43 fqj 050i ONr 4*) flj $4 04 k5* 0 .0r 0V1: 5.4 0 4 (d P40 00 05 4.)v4 04

  3. Three distinct subsets of thymic epithelial cells in rats and mice defined by novel antibodies.

    Directory of Open Access Journals (Sweden)

    Yasushi Sawanobori

    Full Text Available Thymic epithelial cells (TECs are thought to play an essential role in T cell development and have been detected mainly in mice using lectin binding and antibodies to keratins. Our aim in the present study was to create a precise map of rat TECs using antibodies to putative markers and novel monoclonal antibodies (i.e., ED 18/19/21 and anti-CD205 antibodies and compare it with a map from mouse counterparts and that of rat thymic dendritic cells.Rat TECs were subdivided on the basis of phenotype into three subsets; ED18+ED19+/-keratin 5 (K5+K8+CD205+ class II MHC (MHCII+ cortical TECs (cTECs, ED18+ED21-K5-K8+Ulex europaeus lectin 1 (UEA-1+CD205- medullary TECs (mTEC1s, and ED18+ED21+K5+K8dullUEA-1-CD205- medullary TECs (mTEC2s. Thymic nurse cells were defined in cytosmears as an ED18+ED19+/-K5+K8+ subset of cTECs. mTEC1s preferentially expressed MHCII, claudin-3, claudin-4, and autoimmune regulator (AIRE. Use of ED18 and ED21 antibodies revealed three subsets of TECs in mice as well. We also detected two distinct TEC-free areas in the subcapsular cortex and in the medulla. Rat dendritic cells in the cortex were MHCII+CD103+ but negative for TEC markers, including CD205. Those in the medulla were MHCII+CD103+ and CD205+ cells were found only in the TEC-free area.Both rats and mice have three TEC subsets with similar phenotypes that can be identified using known markers and new monoclonal antibodies. These findings will facilitate further analysis of TEC subsets and DCs and help to define their roles in thymic selection and in pathological states such as autoimmune disorders.

  4. Are QSO2 hiding among EROs?


    Brusa, Marcella


    We present the results of a deep (80 ks) XMM-Newton survey of one of the largest sample of near-infrared selected Extremely Red Objects (R-K>5) available to date (~300 objects, Daddi et al. 2000). The fraction of individually detected, X-ray emitting EROs is of the order of ~3.5%, down to F_x > 4x10^-15 cgs and Ks

  5. Selective Oxidation and Reactive Wetting During Hot-Dip Galvanizing of a 1.0 pct Al-0.5 pct Si TRIP-Assisted Steel (United States)

    Bellhouse, E. M.; McDermid, J. R.


    Selective oxidation and reactive wetting during continuous galvanizing were studied for a low-alloy transformation induced plasticity (TRIP)-assisted steel with 0.2 pct C, 1.5 pct Mn, 1.0 pct Al and 0.5 pct Si. Three process atmospheres were tested during annealing prior to galvanizing: 220 K (-53 °C) dew point (dp) N2-20 pct H2, 243 K (-30 °C) dp N2-5 pct H2 and 278 K (+5 °C) dp N2-5 pct H2. The process atmosphere oxygen partial pressure affected the oxide chemistry, morphology and thickness. For the 220 K (-53 °C) dp and 243 K (-30 °C) dp process atmospheres, film and nodule-type manganese, silicon and aluminum containing oxides were observed at the surface. For the 278 K (+5 °C) dp atmosphere, MnO was observed at the grain boundaries and as thicker localized surface films. Oxide morphology, thickness and chemistry affected reactive wetting, with complete wetting being observed for the 220 K (-53 °C) dp and 243 K (-30 °C) dp process atmospheres and incomplete reactive wetting being observed for the 278 K (+5 °C) dp atmosphere. Complete reactive wetting for the 220 K (-53 °C) dp and 243 K (-30 °C) dp process atmospheres was attributed to a combination of zinc bridging of oxides, aluminothermic reduction of surface oxides and wetting of the oxides. Incomplete wetting for the 278 K (+5 °C) dp atmosphere was attributed to localized thick MnO films.

  6. Integrative shell of the program complex MARS (Version 1.0) radiation transfer in three-dimensional geometries

    International Nuclear Information System (INIS)

    Degtyarev, I.I.; Lokhovitskij, A.E.; Maslov, M.A.; Yazynin, I.A.


    The first version of integrative shell of the program complex MARS is written for calculating radiation transfer in the three-dimensional geometries. The integrative shell allows the user to work in convenient form with complex MARS, creat input files data and get graphic visualization of calculated functions. Version 1.0 is adapted for personal computers of types IBM-286,386,486 with operative size memory not smaller than 500K. 5 refs

  7. Analysis of spectral data with rare events statistics

    International Nuclear Information System (INIS)

    Ilyushchenko, V.I.; Chernov, N.I.


    The case is considered of analyzing experimental data, when the results of individual experimental runs cannot be summed due to large systematic errors. A statistical analysis of the hypothesis about the persistent peaks in the spectra has been performed by means of the Neyman-Pearson test. The computations demonstrate the confidence level for the hypothesis about the presence of a persistent peak in the spectrum is proportional to the square root of the number of independent experimental runs, K. 5 refs

  8. Derivation of Candidates for the Combat Casualty Critical Care (C4) Database (United States)


    James D. Oliver, MC USA∥; COL Kevin C. Abbott , MC USA∥; John A. Jones, BS§; LTC Kevin K. Chung, MC USA§ ABSTRACT Objective: To describe the...Cannon J. W., Zonies D. H., Morrow B. D., Orman J. A., Oliver J. D., Abbott K. C., Jones J. A., Chung K. K., 5d. PROJECT NUMBER 5e. TASK NUMBER...Anuria ( cc/24 hour) Use of vasoactive meds Contrasted radiologic studies Insulin requirement Comorbid diagnoses: diabetes , coronary artery disease

  9. U.S. - China Relations: Cooperation or Conflict in the 21st Century (United States)


    Public reporting burden for the collection of information is estimated to average 1 hour per response, including the time for reviewing instructions...NUMBER 6. AUTHOR(S) Scott, Frances K. 5d. PROJECT NUMBER 5e. TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES...Europe and Northern Asia-Pacific in Globalised Governance. Baden-Baden: Nomos, 2011. (JZ 1570 .A55 S73 2011) Wedeman, Andrew. Double Paradox: Rapid

  10. A method to evaluate fission gas release during irradiation testing of spherical fuel - HTR2008-58184

    International Nuclear Information System (INIS)

    Van Der Merwet, H.; Venter, J.


    The evaluation of fission gas release from spherical fuel during irradiation testing is critical to understand expected fuel performance under real reactor conditions. Online measurements of Krypton and Xenon fission products explain coated particle performance and contributions from graphitic matrix materials used in fuel manufacture and irradiation rig materials. Methods that are being developed to accurately evaluate fission gas release are described here together with examples of evaluations performed on irradiation tests HFR-K5, -K6 and EU1bis. (authors)

  11. Spiders in mountain habitats of the Giant Mountains

    Czech Academy of Sciences Publication Activity Database

    Růžička, Vlastimil; Vaněk, J.; Šmilauer, P.


    Roč. 43, č. 4 (2012), s. 341-347 ISSN 1067-4136 Institutional research plan: CEZ:AV0Z50070508 Keywords : Giant Mountains (Krkonoše, Karkonosze) * spider s * anemo-orographic systems Subject RIV: EH - Ecology, Behaviour Impact factor: 0.236, year: 2012

  12. Intermetallic particles-induced pitting corrosion in 6061-T651 aluminium alloy

    CSIR Research Space (South Africa)

    Mutombo, K


    Full Text Available , as shown in Figure 3b. Element Wt% Wt % Error C K 5.22 +/- 0.18 O K 8.32 +/- 0.09 Mg K 0.34 +/- 0.05 Al K 78.26 +/- 0.21 Si K 1.36 +/- 0.07 Fe L 5.18 +/- 0.26 Cu L 1.32 +/- 0.11 Figure 3...

  13. Spatiotemporal Expression of p63 in Mouse Epidermal Commitment

    Directory of Open Access Journals (Sweden)

    Qian Zhao


    Full Text Available The embryonic surface ectoderm is a simple flat epithelium consisting of cells that express the cytokeratins K8/K18. Before stratification, K5/K14 expression substitutes K8/K18 expression, marking the event called epidermal commitment. Previous studies show that the transcription factor p63 plays an essential role in epidermal commitment. However, detailed expression information of p63 during early epidermal development in mice is still unclear. We systematically studied the expression pattern of p63 in mouse epidermal commitment, together with K8 and K5. We show that p63 expression could be detected as early as E8.5 in mouse embryos preceding epidermal commitment. p63 expression first appears near the newly formed somites and the posterior part of the embryo, further expanding to the whole embryonic surface with particular enrichment in the first branchial arches and the limb buds. ΔNp63 is the major class of isoforms expressed in this period. Relative expression intensity of p63 depends on the embryonic position. In summary, there is a sequential and regular expression pattern of K8, p63 and K5 in mouse epidermal commitment. Our study not only contributes to understanding the early events during epidermal development but also provides a basal tool to study the function of p63 in mammals.

  14. Histone Modification Is Involved in Okadaic Acid (OA Induced DNA Damage Response and G2-M Transition Arrest in Maize.

    Directory of Open Access Journals (Sweden)

    Hao Zhang

    Full Text Available Histone modifications are involved in regulation of chromatin structure. To investigate the relationship between chromatin modification and cell cycle regulation during plant cell proliferation, Okadaic acid (OA, a specific inhibitor of serine/threonine protein phosphatase, was applied in this study. The results showed that OA caused the cell cycle arrest at preprophase, leading to seedling growth inhibition. Western blotting assay revealed that the spatial distribution of phosphorylation of Ser10 histone H3 tails (H3S10ph signals was altered under OA treatment. Reactive oxygen species (ROS was found to be at higher levels and TdT-mediated dUTP nick end labeling (TUNEL assay displayed DNA breaks happened at the chromatin after treatment with OA, companied with an increase in the acetylation of histone H4 at lysine 5 (H4K5ac level. From these observations, we speculated that the alteration of the spatial distribution of H3S10ph and the level of H4K5ac was involved in the procedure that OA induced DNA breaks and G2-M arrested by the accumulation of ROS, and that the histone H3S10ph and H4K5ac might facilitate DNA repair by their association with the chromatin decondensation.

  15. Small-angle scattering studies show distinct conformations of calmodulin in its complexes with two peptides based on the regulatory domain of the catalytic subunit of phosphorylase kinase

    International Nuclear Information System (INIS)

    Trewhella, J.; Blumenthal, D.K.; Rokop, S.E.; Seeger, P.A.


    Small-angle X-ray and neutron scattering have been used to study the solution structures of calmodulin complexed with synthetic peptides corresponding to residues 342-366 and 301-326, designated PhK5 and PhK13, respectively, in the regulatory domain of the catalytic subunit of skeletal muscle phosphorylase kinase. The scattering data show that binding of PhK5 to calmodulin induces a dramatic contraction of calmodulin, similar to that previously observed when calmodulin is complexed with the calmodulin-binding domain peptide from rabbit skeletal muscle myosin light chain kinase. In contrast, calmodulin remains extended upon binding PhK13. In the presence of both peptides, calmodulin also remains extended. Apparently, the presence of PhK13 inhibits calmodulin from undergoing the PhK5-induced contraction. These data indicate that there is a fundamentally different type of calmodulin-target enzyme interaction in the case of the catalytic subunit of phosphorylase kinase compared with that for myosin light chain kinase

  16. Anti-inflammatory properties of the medicinal mushroom Cordyceps militaris might be related to its linear (1→3-β-D-glucan.

    Directory of Open Access Journals (Sweden)

    Fhernanda R Smiderle

    Full Text Available The Ascomycete Cordyceps militaris, an entomopathogenic fungus, is one of the most important traditional Chinese medicines. Studies related to its pharmacological properties suggest that this mushroom can exert interesting biological activities. Aqueous (CW and HW and alkaline (K5 extracts containing polysaccharides were prepared from this mushroom, and a β-D-glucan was purified. This polymer was analysed by GC-MS and NMR spectrometry, showing a linear chain composed of β-D-Glcp (1→3-linked. The six main signals in the 13C-NMR spectrum were assigned by comparison to reported data. The aqueous (CW, HW extracts stimulated the expression of IL-1β, TNF-α, and COX-2 by THP-1 macrophages, while the alkaline (K5 extract did not show any effect. However, when the extracts were added to the cells in the presence of LPS, K5 showed the highest inhibition of the pro-inflammatory genes expression. This inhibitory effect was also observed for the purified β-(1→3-D-glucan, that seems to be the most potent anti-inflammatory compound present in the polysaccharide extracts of C. militaris. In vivo, β-(1→3-D-glucan also inhibited significantly the inflammatory phase of formalin-induced nociceptive response, and, in addition, it reduced the migration of total leukocytes but not the neutrophils induced by LPS. In conclusion, this study clearly demonstrates the anti-inflammatory effect of β-(1→3-D-glucan.

  17. Genome-Wide Expression Profiling of Five Mouse Models Identifies Similarities and Differences with Human Psoriasis (United States)

    Swindell, William R.; Johnston, Andrew; Carbajal, Steve; Han, Gangwen; Wohn, Christian; Lu, Jun; Xing, Xianying; Nair, Rajan P.; Voorhees, John J.; Elder, James T.; Wang, Xiao-Jing; Sano, Shigetoshi; Prens, Errol P.; DiGiovanni, John; Pittelkow, Mark R.; Ward, Nicole L.; Gudjonsson, Johann E.


    Development of a suitable mouse model would facilitate the investigation of pathomechanisms underlying human psoriasis and would also assist in development of therapeutic treatments. However, while many psoriasis mouse models have been proposed, no single model recapitulates all features of the human disease, and standardized validation criteria for psoriasis mouse models have not been widely applied. In this study, whole-genome transcriptional profiling is used to compare gene expression patterns manifested by human psoriatic skin lesions with those that occur in five psoriasis mouse models (K5-Tie2, imiquimod, K14-AREG, K5-Stat3C and K5-TGFbeta1). While the cutaneous gene expression profiles associated with each mouse phenotype exhibited statistically significant similarity to the expression profile of psoriasis in humans, each model displayed distinctive sets of similarities and differences in comparison to human psoriasis. For all five models, correspondence to the human disease was strong with respect to genes involved in epidermal development and keratinization. Immune and inflammation-associated gene expression, in contrast, was more variable between models as compared to the human disease. These findings support the value of all five models as research tools, each with identifiable areas of convergence to and divergence from the human disease. Additionally, the approach used in this paper provides an objective and quantitative method for evaluation of proposed mouse models of psoriasis, which can be strategically applied in future studies to score strengths of mouse phenotypes relative to specific aspects of human psoriasis. PMID:21483750

  18. FOG-2 mediated recruitment of the NuRD complex regulates cardiomyocyte proliferation during heart development. (United States)

    Garnatz, Audrey S; Gao, Zhiguang; Broman, Michael; Martens, Spencer; Earley, Judy U; Svensson, Eric C


    FOG-2 is a multi-zinc finger protein that binds the transcriptional activator GATA4 and modulates GATA4-mediated regulation of target genes during heart development. Our previous work has demonstrated that the Nucleosome Remodeling and Deacetylase (NuRD) complex physically interacts with FOG-2 and is necessary for FOG-2 mediated repression of GATA4 activity in vitro. However, the relevance of this interaction for FOG-2 function in vivo has remained unclear. In this report, we demonstrate the importance of FOG-2/NuRD interaction through the generation and characterization of mice homozygous for a mutation in FOG-2 that disrupts NuRD binding (FOG-2(R3K5A)). These mice exhibit a perinatal lethality and have multiple cardiac malformations, including ventricular and atrial septal defects and a thin ventricular myocardium. To investigate the etiology of the thin myocardium, we measured the rate of cardiomyocyte proliferation in wild-type and FOG-2(R3K5A) developing hearts. We found cardiomyocyte proliferation was reduced by 31±8% in FOG-2(R3K5A) mice. Gene expression analysis indicated that the cell cycle inhibitor Cdkn1a (p21(cip1)) is up-regulated 2.0±0.2-fold in FOG-2(R3K5A) hearts. In addition, we demonstrate that FOG-2 can directly repress the activity of the Cdkn1a gene promoter, suggesting a model by which FOG-2/NuRD promotes ventricular wall thickening by repression of this cell cycle inhibitor. Consistent with this notion, the genetic ablation of Cdkn1a in FOG-2(R3K5A) mice leads to an improvement in left ventricular function and a partial rescue of left ventricular wall thickness. Taken together, our results define a novel mechanism in which FOG-2/NuRD interaction is required for cardiomyocyte proliferation by directly down-regulating the cell cycle inhibitor Cdkn1a during heart development. Copyright © 2014 Elsevier Inc. All rights reserved.

  19. Structural Basis of Mec1-Ddc2-RPA Assembly and Activation on Single-Stranded DNA at Sites of Damage. (United States)

    Deshpande, Ishan; Seeber, Andrew; Shimada, Kenji; Keusch, Jeremy J; Gut, Heinz; Gasser, Susan M


    Mec1-Ddc2 (ATR-ATRIP) is a key DNA-damage-sensing kinase that is recruited through the single-stranded (ss) DNA-binding replication protein A (RPA) to initiate the DNA damage checkpoint response. Activation of ATR-ATRIP in the absence of DNA damage is lethal. Therefore, it is important that damage-specific recruitment precedes kinase activation, which is achieved at least in part by Mec1-Ddc2 homodimerization. Here, we report a structural, biochemical, and functional characterization of the yeast Mec1-Ddc2-RPA assembly. High-resolution co-crystal structures of Ddc2-Rfa1 and Ddc2-Rfa1-t11 (K45E mutant) N termini and of the Ddc2 coiled-coil domain (CCD) provide insight into Mec1-Ddc2 homodimerization and damage-site targeting. Based on our structural and functional findings, we present a Mec1-Ddc2-RPA-ssDNA composite structural model. By way of validation, we show that RPA-dependent recruitment of Mec1-Ddc2 is crucial for maintaining its homodimeric state at ssDNA and that Ddc2's recruitment domain and CCD are important for Mec1-dependent survival of UV-light-induced DNA damage. Copyright © 2017 Elsevier Inc. All rights reserved.

  20. Agent Communication for Dynamic Belief Update (United States)

    Kobayashi, Mikito; Tojo, Satoshi

    Thus far, various formalizations of rational / logical agent model have been proposed. In this paper, we include the notion of communication channel and belief modality into update logic, and introduce Belief Update Logic (BUL). First, we discuss that how we can reformalize the inform action of FIPA-ACL into communication channel, which represents a connection between agents. Thus, our agents can send a message only when they believe, and also there actually is, a channel between him / her and a receiver. Then, we present a static belief logic (BL) and show its soundness and completeness. Next, we develop the logic to BUL, which can update Kripke model by the inform action; in which we show that in the updated model the belief operator also satisfies K45. Thereafter, we show that every sentence in BUL can be translated into BL; thus, we can contend that BUL is also sound and complete. Furthermore, we discuss the features of CUL, including the case of inconsistent information, as well as channel transmission. Finally, we summarize our contribution and discuss some future issues.

  1. Alterations in protein synthesis in the cyanobacterium Synechococcus sp. Strain PCC 6301 in response to Calendula Micrantha extract with the Molluscicidal activity

    International Nuclear Information System (INIS)

    Hammouda, O.H.E.; Borbely, G.


    The response to the extract of the egyptian wild herb Calendula Micrantha, with the Molluscicidal activity, was examined in the unicellular no bacterium Synechococcus sp. strain PCC 6301. growth and chlorophyll of the cells were only slightly affected by low plant extract concentrations but were drastically reduced by high concentration. the rate of protein synthesis progressively decreased by increasing extract concentration. the cells preferentially induced the synthesis of a limited number of polypeptides in response to the treatment. Among the induced polypeptides were those with apparent molecular weights of 161 K (161.000), 96.7 K, 93.4 K, 69.9 K, 59 K, 49 K, 45 K, 35 K, 32.4 K, 28 K, 24 K, 21.7 K, 18 K and 16 K based on their mobilities in gel electrophoresis. these initial studies suggest that the plant extract exerted certain stress which stimulated alteration in the pattern of protein synthesis in Synechococcus sp. some of induced polypeptides are similar to that known to occur in other stresses specially heat shock stress. 3 figs

  2. Alterations in protein synthesis in the cyanobacterium Synechococcus sp. Strain PCC 6301 in response to Calendula Micrantha extract with the Molluscicidal activity.

    Energy Technology Data Exchange (ETDEWEB)

    Hammouda, O H.E. [Botany Department, Faculty of Science, Cairo University, Beni-Suef branch. Beni Suef (Egypt); Borbely, G [Institute of plant physiology, Biological Research Center, Szeged H-6701, (Hungary)


    The response to the extract of the egyptian wild herb Calendula Micrantha, with the Molluscicidal activity, was examined in the unicellular no bacterium Synechococcus sp. strain PCC 6301. growth and chlorophyll of the cells were only slightly affected by low plant extract concentrations but were drastically reduced by high concentration. the rate of protein synthesis progressively decreased by increasing extract concentration. the cells preferentially induced the synthesis of a limited number of polypeptides in response to the treatment. Among the induced polypeptides were those with apparent molecular weights of 161 K (161.000), 96.7 K, 93.4 K, 69.9 K, 59 K, 49 K, 45 K, 35 K, 32.4 K, 28 K, 24 K, 21.7 K, 18 K and 16 K based on their mobilities in gel electrophoresis. these initial studies suggest that the plant extract exerted certain stress which stimulated alteration in the pattern of protein synthesis in Synechococcus sp. some of induced polypeptides are similar to that known to occur in other stresses specially heat shock stress. 3 figs.

  3. Thyroid hormone levels in patients with chronic renal failure under haemodialysis

    International Nuclear Information System (INIS)

    Khalid, Ahmed Shukralla M.


    This study was conducted with three main objectives, to study thyroid hormones (T 4 , T 3 ) and TSH levels in patients with CRF under haemodialysis and to compare them with normal subjects, to study best means of treatment and to compare these findings with results from other parts of the world. This study was done on 61 patients with renal failure in Khartoum dialysis and kidney transplant centre U of K, 45 males and 16 females with ages ranging from 17-75 years and 42 symptoms-free subjects 14 males and 23 females with age ranging from 16-60 years. The radioimmunoassay (RIA) technique was used for the determination of serum T 4 , T 3 and TSH. By using t-test found that the mean concentrations of T 4 , T 3 of normal subjects were much higher than those of the patients (p 0.05). These results also illustrated that 45.9% of patients with renal failure of low T 4 , and 91.8%, 90.26 of patients had T 3 and TSH hormone levels in the normal range, respectively. No significant difference was observed in the mean of thyroid hormones (T 4 , T 3 ) and thyroid-stimulating hormones between males and females (p>0.05). The T 3 and T '4 concentrations in patients at all age groups (year) was less than the age groups of the control group, and this decrease was statistically significant (p 0.05).(Author)

  4. The anti-tumor efficacy of 3C23K, a glyco-engineered humanized anti-MISRII antibody, in an ovarian cancer model is mainly mediated by engagement of immune effector cells. (United States)

    Estupina, Pauline; Fontayne, Alexandre; Barret, Jean-Marc; Kersual, Nathalie; Dubreuil, Olivier; Le Blay, Marion; Pichard, Alexandre; Jarlier, Marta; Pugnière, Martine; Chauvin, Maëva; Chardès, Thierry; Pouget, Jean-Pierre; Deshayes, Emmanuel; Rossignol, Alexis; Abache, Toufik; de Romeuf, Christophe; Terrier, Aurélie; Verhaeghe, Lucie; Gaucher, Christine; Prost, Jean-François; Pèlegrin, André; Navarro-Teulon, Isabelle


    Ovarian cancer is the leading cause of death in women with gynecological cancers and despite recent advances, new and more efficient therapies are crucially needed. Müllerian Inhibiting Substance type II Receptor (MISRII, also named AMHRII) is expressed in most ovarian cancer subtypes and is a novel potential target for ovarian cancer immunotherapy. We previously developed and tested 12G4, the first murine monoclonal antibody (MAb) against human MISRII. Here, we report the humanization, affinity maturation and glyco-engineering steps of 12G4 to generate the Fc-optimized 3C23K MAb, and the evaluation of its in vivo anti-tumor activity. The epitopes of 3C23K and 12G4 were strictly identical and 3C23K affinity for MISRII was enhanced by a factor of about 14 (KD = 5.5 × 10-11 M vs 7.9 × 10-10 M), while the use of the EMABling® platform allowed the production of a low-fucosylated 3C23K antibody with a 30-fold KD improvement of its affinity to FcγRIIIa. In COV434-MISRII tumor-bearing mice, 3C23K reduced tumor growth more efficiently than 12G4 and its combination with carboplatin was more efficient than each monotherapy with a mean tumor size of 500, 1100 and 100 mm3 at the end of treatment with 3C23K (10 mg/kg, Q3-4D12), carboplatin (60 mg/kg, Q7D4) and 3C23K+carboplatin, respectively. Conversely, 3C23K-FcKO, a 3C23K form without affinity for the FcγRIIIa receptor, did not display any anti-tumor effect in vivo. These results strongly suggested that 3C23K mechanisms of action are mainly Fc-related. In vitro, antibody-dependent cytotoxicity (ADCC) and antibody-dependent cell phagocytosis (ADCP) were induced by 3C23K, as demonstrated with human effector cells. Using human NK cells, 50% of the maximal lysis was obtained with a 46-fold lower concentration of low-fucosylated 3C23K (2.9 ng/ml) than of 3C23K expressed in CHO cells (133.35 ng/ml). As 3C23K induced strong ADCC with human PBMC but almost none with murine PBMC, antibody-dependent cell phagocytosis (ADCP) was

  5. Prevention of propofol-induced pain in children: pretreatment with small doses of ketamine. (United States)

    Zhao, Guang-yi; Guo, Yao; Bao, Shu-min; Meng, Ling-xin; Zhang, Li-hong


    To evaluate the efficacy of ketamine in preventing propofol injection pain in children. Prospective, randomized, double-blinded, placebo-controlled study. University-affiliated hospital. 192 ASA physical status 1 and 2 pediatric patients. Patients were randomly assigned to 4 groups. Group S (control) received normal saline as a placebo; Group K1, Group K3, and Group K5 received 0.1 mg/kg, 0.3 mg/kg, and 0.5 mg/kg of ketamine, respectively. Fifteen seconds after the ketamine injection, patients were injected with propofol at a rate of 12 mL/min until loss-of-eyelash reflex. Pain was evaluated blindly at the time of induction using a 4-point scale: 0 = no pain, 1 = mild pain, 2 = moderate pain, and 3 = severe pain. Adverse effects were recorded. Characteristics of induction of anesthesia, such as dose of propofol and time from propofol injection to loss of consciousness (induction duration), were noted. 39 (84.8%) Group S (control) patients had pain. Pretreatment with ketamine reduced the frequency of pain significantly to 56.5%, 17.0%, and 14.9% in Groups K1, K3, and K5, respectively. Furthermore, the frequency of moderate and severe pain in Group K1 (21.8%), Group K3 (6.4%), and Group K5 (4.3%) was significantly (P ketamine (0.3 mg/kg) reduced the frequency and intensity of propofol injection pain without severe adverse effects. Copyright © 2012 Elsevier Inc. All rights reserved.


    Directory of Open Access Journals (Sweden)

    Alfi Muntafiah


    Full Text Available Diabetes mellitus (DM is a disease characterized by elevated blood glucose levels (hyperglycemia caused by deficiency of insulin, and insulin resistance or both. This chronic disease prevalence is increasing nationally and globally. This study aimed to determine the effect of ginger extract and honey various doses on levels of total cholesterol in the Wistar diabetic rat model induced by alloxan. This research is true experimental post-test only with control group design. Subject of the study 30 male Wistar rats weight 150-200 grams, divided into 6 groups: A healthy controls (K1, B DM control (K2, C Treatment with red ginger extract 1000 mg / kg and honey 1 ml / kg (K3, D Treatment with ginger extract red 1000 mg / kg and honey 2 ml / kg (K4, E Treatment with red ginger extract 500 mg / kg and honey 1 ml / kg (K5, F Treatment with red ginger extract 500 mg / kg and honey 2 ml / kg (K6. DM induction by alloxan 160 mg / kg intraperitoneally for 5 days, and the provision of treatment for 14 days. Total cholesterol levels were measured by CHOD-PAP method. Results: The mean total cholesterol levels of healthy control group vs the diabetic control 58.20 ± 8.76 vs. 87.80 ± 5.81 mg / dL. Based on one way ANOVA test, red ginger extract and honey various doses significantly lower total cholesterol level (p <0.05. The mean total cholesterol levels between the group K3 to K4 was not statistically different, as well as K5 with K6. However, mean total cholesterol levels at K3 and K4 differ significantly from the K5 and K6. Conclusion: Combination of red ginger extract and honey can lower total cholesterol levels in diabetic rat model induced by alloxan.

  7. A comparative study on the fretting wear properties of advanced zirconium fuel cladding materials

    International Nuclear Information System (INIS)

    Lee, Young Ho; Kim, Hyung Kyu; Park, Jeong Yong; Kim, Jun Hwan


    Fretting wear tests were carried out in room and high temperature water in order to evaluate the wear properties of new zirconium nuclear fuel claddings (K2∼K6) and the commercial claddings (M5, zirlo and zircaloy-4). The objective is to compare the wear resistance of K2∼K6 claddings with that of the commercial ones at the same test condition. After the wear tests, the average wear volume and the maximum wear depth were evaluated and compared at each test condition. As a result, it is difficult to select the most wear-resistant cladding between the K2∼K6 claddings and the commercial ones. This is because the average wear volume and maximum depth of each cladding included between the scattering range of measured results. However, wear resistance of the tested claddings based on the average wear volume and maximum wear depth could be summarized as follows: K5 > zircaloy-4 > (K2,K3) > (K4,M5) > K6 > zirlo at room temperature, zircaloy-4 > K5 > (K3,K4,zirlo) > (K2,K6) > M5 at high temperature and pressure. Therefore, it is concluded that K5 cladding among the tested new zirconium alloys has relatively higher wear-resistance in room and high temperature condition. In order to examine the wear mechanism, it is necessary to systematically study with the consideration of the alloying element effect and test environment. In this report, the wear test procedure and the wear evaluation method are described in detail

  8. Thermodynamics of the extraction of Eu(III) and Am(III) with synergistic mixtures of thenoyltrifluoroacetone and a linear polyether

    International Nuclear Information System (INIS)

    Ensor, D.D.; Shah, A.H.


    The extractions of trivalent lanthanides and actinides by synergistic mixtures of thenoyltrifluoroacetone (HTTA) and a variety of neutral oxygen donors are well documented. The large increase in the extent of extraction of the metal ion into the organic phase is believed to occur by one of three possible mechanisms. The first involves the direct replacement of residual waters of hydration on the metal by the neutral donor. In the second proposed mechanism, the residual waters remain attached to the metal and the neutral oxygen donor hydrogen bond to the complex through the waters. The final mechanism assumes the complex is not hydrated and the addition of the neutral donor requires the metal to increase its coordination number. A recent review of the synergistic effect showed that the proper interpretation of the mechanism requires the measurement of ΔH and ΔS for the reaction. The synergistic capabilities of a linear polyether, 1,13-bis-[8-quinolyl]-1,4,7,10,13-pentaoxotridecane (K-5), in combination with HTTA to extract Am(III) and Eu(III) have been recently reported by the investigators. The presence of the K-5 in the organic phase not only increased the extraction of the trivalent metals, but also showed a preference for Am(III) over Eu(III). This result was surprising since most neutral donors do not alter the selectivity of HTTA. In an effort to better understand the synergistic mechanism of the K-5/HTTA system, the thermodynamic parameters have been measured by a temperature variation method, and results are reported in this paper. 7 references

  9. Involvement of TNF-α converting enzyme in the development of psoriasis-like lesions in a mouse model.

    Directory of Open Access Journals (Sweden)

    Kenji Sato

    Full Text Available TNF-α plays a crucial role in psoriasis; therefore, TNF inhibition has become a gold standard for the treatment of psoriasis. TNF-α is processed from a membrane-bound form by TNF-α converting enzyme (TACE to soluble form, which exerts a number of biological activities. EGF receptor (EGFR ligands, including heparin-binding EGF-like growth factor (HB-EGF, amphiregulin and transforming growth factor (TGF-α are also TACE substrates and are psoriasis-associated growth factors. Vascular endothelial growth factor (VEGF, one of the downstream molecules of EGFR and TNF signaling, plays a key role in angiogenesis for developing psoriasis. In the present study, to assess the possible role of TACE in the pathogenesis of psoriasis, we investigated the involvement of TACE in TPA-induced psoriasis-like lesions in K5.Stat3C mice, which represent a mouse model of psoriasis. In this mouse model, TNF-α, amphiregulin, HB-EGF and TGF-α were significantly up-regulated in the skin lesions, similar to human psoriasis. Treatment of K5.Stat3C mice with TNF-α or EGFR inhibitors attenuated the skin lesions, suggesting the roles of TACE substrates in psoriasis. Furthermore, the skin lesions of K5.Stat3C mice showed down-regulation of tissue inhibitor of metalloproteinase-3, an endogenous inhibitor of TACE, and an increase in soluble TNF-α. A TACE inhibitor abrogated EGFR ligand-dependent keratinocyte proliferation and VEGF production in vitro, suggesting that TACE was involved in both epidermal hyperplasia and angiogenesis during psoriasis development. These results strongly suggest that TACE contributes to the development of psoriatic lesions through releasing two kinds of psoriasis mediators, TNF-α and EGFR ligands. Therefore, TACE could be a potential therapeutic target for the treatment of psoriasis.

  10. Preparation, characterization and photocatalytic applications of amine-functionalized mesoporous silica impregnated with transition-metal-monosubstituted polyoxometalates

    International Nuclear Information System (INIS)

    Li Li; Liu, Chunming; Geng Aifang; Jiang Chunjie; Guo Yihang; Hu Changwen


    Amine-functionalized mesoporous silica materials impregnated with transition-metal-monosubstituted polyoxometalates, K 5 [M(H 2 O)PW 11 O 39 ]-(EtO) 3 SiCH 2 CH 2 CH 2 NH 2 -MCM-48 (M = Co/Ni), were prepared by coordination of nickel/cobalt centers in the clusters with the amine surface groups in amine-functionalized mesoporous silica supports. The materials obtained were characterized by powder X-ray diffraction (XRD), UV-vis diffuse reflectance spectra (UV-vis-DR), infrared (IR) spectra, magic-angle spinning 31 P MAS NMR, transmission electron microscopy (TEM) and nitrogen adsorption measurements, indicating that the primary Keggin structures remained intact in as-prepared composites, and the composites possessed mesoporous structures. The composites exhibited UV-photocatalytic activity to degrade dye rhodamine B (RB), and the pesticides including hexachlorobenzene (HCB) and methylparathion (MPT). Leakage of K 5 [M(H 2 O)PW 11 O 39 ] from the support was hardly observed during the photocatalytic tests, attributed to strong coordination interactions between the Keggin units and the amine-functionalized silica surface. -- Graphical abstract: The K 5 [M(H 2 O)PW 11 O 39 ]-(EtO) 3 SiCH 2 CH 2 CH 2 NH 2 -SiO 2 composites were prepared by coordination of M centers in the Keggin units with the amine surface groups in amine-functionalized mesoporous silica supports, and the composites exhibited photocatalytic activity to degrade aqueous rhodamine B, hexachlorobenzene and methyl parathion

  11. On inertial range scaling laws

    International Nuclear Information System (INIS)

    Bowman, J.C.


    Inertial-range scaling laws for two- and three-dimensional turbulence are re-examined within a unified framework. A new correction to Kolmogorov's k -5/3 scaling is derived for the energy inertial range. A related modification is found to Kraichnan's logarithmically corrected two-dimensional enstrophy cascade law that removes its unexpected divergence at the injection wavenumber. The significance of these corrections is illustrated with steady-state energy spectra from recent high-resolution closure computations. The results also underscore the asymptotic nature of inertial-range scaling laws. Implications for conventional numerical simulations are discussed

  12. Observations on the variability of linear polarization in late-type dwarf stars

    Energy Technology Data Exchange (ETDEWEB)

    Huovelin, J.; Linnaluoto, S.; Tuominen, I.; Virtanen, H.


    Broadband (UBV) linear polarimetric observations of a sample of late-type (F7-K5) dwarfs are reported. The observations include ten stars and extend over a maximum of 20 nights. Seven stars show significant temporal variability of polarization, which could be interpreted as rotational modulation due to slowly varying magnetic regions. Magnetic intensification in saturated Zeeman sensitive absorption lines is suggested as the dominant effect connecting linear polarization with magnetic activity in the most active single late-type dwarfs, while the wavelength dependence in the less active stars could also be due to a combination of Rayleigh and Thomson scattering.

  13. Nanospectrofluorometry inside single living cell by scanning near-field optical microscopy (United States)

    Lei, F. H.; Shang, G. Y.; Troyon, M.; Spajer, M.; Morjani, H.; Angiboust, J. F.; Manfait, M.


    Near-field fluorescence spectra with subdiffraction limit spatial resolution have been taken in the proximity of mitochondrial membrane inside breast adenocarcinoma cells (MCF7) treated with the fluorescent dye (JC-1) by using a scanning near-field optical microscope coupled with a confocal laser microspectrofluorometer. The probe-sample distance control is based on a piezoelectric bimorph shear force sensor having a static spring constant k=5 μN/nm and a quality factor Q=40 in a physiological medium of viscosity η=1.0 cp. The sensitivity of the force sensor has been tested by imaging a MCF7 cell surface.

  14. Risk factors, diagnosis and non-surgical treatment for meniscal tears

    DEFF Research Database (Denmark)

    Thorlund, Jonas Bloch; Juhl, Carsten Bogh; Ingelsrud, Lina Holm


    quantitatively or qualitatively, as appropriate. Strength of evidence was determined according to the Grading of Recommendations Assessment Development and Evaluation framework. Low-quality evidence suggested that overweight (degenerative tears, k=3), male sex (k=4), contact and pivoting sports (k=2...... investigated exercise versus surgery (k=2) or the effect of surgery in addition to exercise (k=5) for degenerative meniscal tears. There was moderate level of evidence for exercise improving self-reported pain (Effect Size (ES)-0.51, 95% CI -1.16 to 0.13) and function (ES -0.06, 95% CI -0.23 to 0...

  15. Agreement of non-invasive tear break up time measurement between Tomey RT-7000 Auto Refractor-Keratometer and Oculus Keratograph 5M

    Directory of Open Access Journals (Sweden)

    Lee R


    Full Text Available Ryan Lee,1,2 Sharon Yeo,1 Han Tun Aung,3 Louis Tong1,2,4,5 1Ocular Surface Research Group, Singapore Eye Research Institute, 2Yong Loo Lin School of Medicine, National University of Singapore, 3School of Health Sciences, Ngee Ann Polytechnic, 4Duke-NUS Medical School, Singapore; 5Cornea and External Eye Disease Service, Singapore National Eye Centre, Singapore Background: It is difficult to standardize assessment of dry eye in different clinical settings. Increasingly, tear stability is recognized to be important for the definition and assessment of patients with dry eye. Recently, two commercially available instruments have been made available for objectively measuring noninvasive tear break-up time (NIBUT, as an indicator of tear stability: the Tomey RT-7000 Auto Refractor-Keratometer and Oculus Keratograph (K5M. We aim to assess the agreement of NIBUT measurements using these modalities.Methods: This prospective cross-sectional study was carried out in a tertiary referral eye center and involved 126 consecutive dry eye patients. NIBUT assessment was performed on the right eyes of participants with both the RT-7000 and the K5M techniques, with the order of assessment randomized. The Standardized Patient Evaluation of Eye Dryness (SPEED questionnaires were administered to assess dry eye symptoms in the 2 weeks before assessment.Results: The age of the participants was 56.0±14.3 years (69.84% females. Measurements for both modalities were non-normally distributed (right-skewed. The median RT-7000 and K5M readings were 4.2 (range 0.1–10.0 and 6.4 (0.1–24.9 seconds, respectively. RT-7000 and K5M readings were poorly correlated (ρ=0.061, P=0.495. Intraclass correlation coefficient between the modalities was 0.187 (95% confidence interval -0.097 to 0.406. The Bland–Altman plot showed no systematic differences between the readings with these machines. The agreement between machines was not different in different SPEED categories

  16. Proceedings of the DARPA/AFML Review of Progress in Quantitative Nondestructive Evaluation, 8-13 July, La Jolla, California. (United States)


    trade -off -- there’s mathematical limitations still. If you were to use something where there is some time coding. I’m thinking of specifically phbse...the equipment. Experimentally, spection of the pipe. the trade offs between results and experimental con- Again radiographic film was used as the...pj.,except forex = inc + )apt m p~m (I) the wavenumber, which is k’ rather than k. 5 in - b p 9p"m (2) Hooke’s law for the elastic solid is embodied

  17. OPERATION CASTLE. The Operation Plan Number 1-53. Task Group 7.1 (United States)


    83-C-0286 with the close cooperation of the Classification • Management Division of the Defense Nuclear Agency. 19 KEY WORDS (Conltnue on reverse side...Day on which k5th Shot) will be detonated. R-DAY (RC*(E.) - fay on which (6th Shot) will be detonated. -5- I o K-DAY ( KOON ) - Day on which (7th Shot...timing signals to meet the principle requirements of the experimental prograraz. (2) Supplies the firing pulse to the device(s) to be tested. (3

  18. Compactibility of atomized high-speed steel and steel 3 powders

    International Nuclear Information System (INIS)

    Kulak, L.D.; Gavrilenko, A.P.; Pikozh, A.P.; Kuz'menko, N.N.


    Spherical powders and powders of lammellar-scaly shape of high-speed R6M5K5 steel and steel 3 produced by the method of centrifugal atomization of a rotating billet under conditions of cold pressing in steel moulds are studied for thier compactability. Compacting pressure dependnences are establsihed for density of cold-pressed compacts of spherical and scaly powders. The powders of lammellar-scaly shape both of high-speed steel and steel 3 are found to possess better compactibility within a wide range of pressures as compared to powders of spherical shape. Compacts of the lammellar-scaly powders possess also higher mechanical strength

  19. Reaction Engineering of Biocatalytic Enantioselective Reduction: A Case Study for Aliphatic Ketones

    DEFF Research Database (Denmark)

    Leuchs, Susanne; Lima-Ramos, Joana; Greiner, Lasse


    , 15, 167–176.). In the present work, the process metrics of the ketone reduction were calculated. A cost analysis revealed that the enzyme costs are negligible, but the cost for nicotinamide cofactor NADP+ is dominating the overall cost of the chemical raw material followed by the ionic liquid (TEGO...... IL K5) used as solubiliser and the buffer. The overall cost of chemicals was €148/kgproduct. To assess the environmental impact of the process, the E-factor (kgwaste/kgproduct) 132 and the process mass intensity 133 (PMI, kgsubstrate/kgproduct) were calculated. A process model based on initial rate...

  20. Distribution of iodine between water and steam: a reassessment of experimental data on hypoiodous acid

    International Nuclear Information System (INIS)

    Turner, D.J.


    A re-analysis has been made of published data on the steam/ water distribution of iodine between 118 0 and 287 0 C. The analysis assumes that the principal reactions are as follows: I 2 + H 2 O = HIO + H + + I - 3I 2 + 3H 2 O = IO 3 - + 5I - + 6H + for which the equilibrium constants are respectively K 2 and K 5 . The analysis of the experimental data was supported by using empirically and theoretically based equations which describe the temperature dependence of equilibrium constants and by comparing predicted behaviour with the observations reported from a number of boiling water reactors. (author)

  1. Management of human-induced salinization in the Berg River catchment (South Africa)

    CSIR Research Space (South Africa)

    Jovanovic, Nebojsa


    Full Text Available Impacts on Salinity in Western Cape Waters. Water Research Commission Report No. K5/1503, Pretoria, South Africa. In press. Figure 1: Location and 20 m Digital Elevation Model of the Berg River basin. The red square indicates the approximate position..., and German Federal Ministry for Education and Research (BMBF) for funding, SA Rock Drill, Caren Jarmain (University of KwaZulu Natal), University of the Western Cape, farmers, United States Geological Survey Historic land uses: ?Pre-1700 savanna, nomadic...

  2. Pulse radiolysis studies of intramolecular electron transfer in model peptides and proteins. 7. Trp -> TyrO radical transformation in hen egg-white lysozyme. Effects of pH, temperature, Trp62 oxidation and inhibitor binding

    DEFF Research Database (Denmark)

    Bobrowski, K.; Holcman, J.; Poznanski, J.


    Intramolecular long-range electron transfer (LRET) in hen egg-white lysozyme (HEWL) accompanying Trp --> TyrO radical transformation was investigated in aqueous solution by pulse radiolysis as a function of pH (5.2-7.4) and temperature (283-328K). The reaction was induced by highly selective...... below its denaturation temperature. Selective oxidation by ozone of the Trp62 indole side-chain in HEWL to N'-formylkynurenine (NFKyn62-HEWL) caused a large drop in the initial yield of Trp(.) radicals, G(Trp(.))(i). This was accompanied by a relatively small decrease in k(5) but selective oxidation...

  3. Environmental Impact Statement Space Shuttle Program, Vandenberg AFB, California. Supplement. (United States)


    2 of the Northern Channel Islands, have been motivated in part by concerns identified through interaction between the Air Force and the Coastal...ofA.SrZ 64 0 /OC 2SS - So#e.e. V, f-rrAq ~ WA~oJsie4,e~4 A-I 4&Ajt4 A fLA1//.t"lJAde~ R-SeI~u- dw rwy. £A4Pcr SL3*p~j* ps4 .Prm Al-fdc6k -5)’-ArFkAJ /7

  4. Thermoregulatory Responses to Intermittent Exercise Are Influenced by Knit Structure of Underwear (United States)


    determined at 120 min, when Swo,(K3) and Swt(K4) were lower compared to both Sw,,(K2) and Swot (K5) 400 (Fig. 3). 300 Skin wettednessEE The knit...dew Pugh LGC (1966) Clothing insulation and accidential hypo- point temperature sensor. J Appl Physiol 52: 1658- 1660 thermia in youth. Nature 209: 1281...insulation. J Appl Physiol 8:539-545 cise and thermal stress. Physiol Rev 54:75-159 Hardy JI), )uBois EF (1938) Basal metabolism, radiation, Saltin B

  5. Supralinear detectors in neutron dosimetry

    International Nuclear Information System (INIS)

    Larsson, L.; Roth, R.A.; Katz, R.


    Dose-response curves for nuclear emulsions exposed to x-rays and neutrons are presented and discussed. Ilford K.5 plates were used to mimic an initial slope model of biological cell survival curves, and Ilford K-2.5 plates were used to mimic the multi-target survival model after gamma-ray irradiation. The plates were exposed to x-rays from a Torrex-150 x-ray unit and fission neutrons at the 18 kW Triga Mark I reactor. Representative calculations for the response of model detectors to 14 MeV neutrons were made for comparison with experimental findings. Results are presented and discussed

  6. Heavy Hyperfragments produced by 800 MeV/c k in Nuclear Emulsions; Hiperfragmentos pesados producidos por K de 800 MeV c en emulsiones nucleares

    Energy Technology Data Exchange (ETDEWEB)

    Marcial, P


    A statistical and phenomenological survey of nearly 1200 heavy hyperfragments produced by interaction of 800 MeV/c K with the heavy nuclei of llford K{sub 5} emulsion is presented. The emulsion was exposed A statistical and phenomenological survey of nearly 1200 heavy hyperfragments produced by interaction of 800 MeV/c K with the heavy nuclei of llford K5 emulsion is presented. The emulsion was exposed in Berkeley. The variation of long list of parameters dealing with both the production and desintegration of the hyperfragments, with the size of the primary interaction is given. (Author)

  7. Unimolecular Reactions of Nitrites and Nitrates. (United States)


    verified the mechanism as being the one originally proposed by Levy, RONO - RO + NO RO + NO- 1 2*RONO •I kRO + NO-- ROH HHO -Hi k5 :and not by direct...produced by ,’Levy’s mechanism. I1 Emission from CH30, C2H50, and l-C3H70 radicals were observed in the photolysis of these nitrites between...wavelengths of 2000 and 1100 A, by Ohbayashi, Akimoto and Tanaka [78]. Emission was assigned to the (A2A1IX2E) transition of CH30 . Bands of NO were also *i

  8. Magnetic effects in cellular and molecular systems

    International Nuclear Information System (INIS)



    Brief discussions are presented of six aspects of the phenomenon. Individual topics discussed include: (1) mechanisms of magnetic field interactions with retinal rods; (2) orientation of biological membranes and cells in magnetic fields; (3) enzyme-substrate reactions in high magnetic fields; (4) effects on cell function resulting from exposure to strong magnetic fields at 4 0 K; (5) effects of a transverse magnetic field on the dose distribution of high energy electrons and in the responses of mammalian cells in vitro to x rays; and (6) effect of magnetic fields on the drug-induced contractility of the ciliate Spirostomum

  9. Astrometry With the Hubble Space Telescope: Trigonometric Parallaxes of Planetary Nebula Nuclei NGC 6853, NGC 7293, ABELL 31, and DeHt 5 (United States)


    Consortium. 1974 BENEDICT ET AL. Vol. 138 8 6 4 2 0 -2 -4 -6 -8 R ed uc ed P ro pe r M ot io n (H K = K + 5 lo g( μ) ) J-K ( 2Mass ) 2004) and J, K photometry from 2MASS for a one-degree-square field centered on DeHt 5. With final proper motions from our astrometric solution...Orientation to the sky is obtained from ground-based as- trometry from 2MASS with uncertainties in the field orientation ±0.◦05. This value, too, was

  10. Orbit and Stellar Properties of the Young Triple V807 Tau (United States)


    Kharchenko & Roeser 2009 (V band); (2) Cutri et al. 2003 ( 2MASS J, H, Ks); (3) White & Ghez 2001 (V, K); (4) flux ratio (A:Ba:Bb) combined with Johnson V...All-sky compiled Catalog (Kharchenko & Roeser 2009) and 2MASS J, H, K; (5) calibrated FGS; (6) flux ratio (Ba:Bb) combined with White & Ghez (2001...K−L color in excess of 0.4 (e.g., Prato et al. 2003). Convert- ing the Two Micron All Sky Survey ( 2MASS ) Ks magnitudes to the Bessell–Brett

  11. Characterization of Five Fungal Endophytes Producing Cajaninstilbene Acid Isolated from Pigeon Pea [Cajanus cajan (L.) Millsp.


    Gao, Yuan; Zhao, Jin Tong; Zu, Yuan Gang; Fu, Yu Jie; Wang, Wei; Luo, Meng; Efferth, Thomas


    Five fungal endophytes (K4, K5, K6, K9, K14) producing Cajaninstilbene acid (CSA, 3-hydroxy-4-prenyl-5-methoxystilbene-2-carboxylic acid) were isolated from the roots of pigeon pea [Cajanus cajan (L.) Millsp.]. CSA is responsible for the prominent pharmacological activities in pigeon pea. The amount of CSA in culture solution varied among the five fungal endophytes. K4 produced the highest levels of CSA (1037.13 µg/L) among the endophytes tested after incubation for five days. Both morphologi...

  12. Sterilization of the artificial blood circulation apparatus with ozone

    Energy Technology Data Exchange (ETDEWEB)

    Pisarevskii, A A; Korukhov, N I.U.; Brazhnikov, E M; Konstantinova, M L; Razumovskii, S D


    Presented in this paper is the sterilization method of the assisted circulation unit. The method has been approved by stand testing and then applied for sterilizing the unit Ae, yeK-5 m when experimental implanting artificial heart. According to the method, ozone in concentration of 5 x 10(-3) moles/1 and with flow rate of 0,5 1/min is passed through an internal circuit of the unit during I h and 15 min. Control wash-out samples from lines have demonstrated complete sterility of the internal circuit. The method makes the sterilization process of such units much easier and may be recommended for clinical application.

  13. Field Surveys, IOC Valleys. Volumes II-I and II-II. Biological Resources Survey, Dry Lake Valley, Nevada and Pine and Wah Wah Valleys, Utah. Supplement. Spring Survey of the IOC Valleys. (United States)


    pinnata X K CACTACEAE Coryphantha vivipara x Opuntia echinocarpa K 0 x K K K 5putaerinacea K Op’untia sp. x X -12- TABLE 3-1 (Cont.) Shelter site...pilosus x Lepidium montanun X CACTACEAE Opuntia echinocarpa x x Opuntia erinacea X X X CHENOPODIACEAE Atriplex canescens X X X Ceratoides lanata X X X X...Stanleya pinnata X CACTACEAE Coryphantha vivipara var. rosea X Echinocereus engelmannii X opuntia echinocarpa X X X X X X X X Opuntia erinacea XX X X X X x

  14. Paradoxical gap in the relative ages of T Tauri stars

    International Nuclear Information System (INIS)

    Weaer, W.B.


    The frequency distribution of T Tauri stars of different Youth (relative age) shows a pronounced gap at 5% of their time to the zero-age main sequence. This gap, which occurs in all of the four major T Tauri associations, is too large to be filled by unclassifiable veiled stars. It is nearly vertical on the Hertzsprung-Russell diagram, is centered near spectral class K5, and lies close to the transition between the convective and radiative tracks of the pre-main-sequence stars

  15. Generalization of measurements of local and integral heat transfer while transverse stream-lining of smooth and rough cylinders

    International Nuclear Information System (INIS)

    Akhenbakh, Eh.


    The investigation is presented into heat transfer in the boundary layer and in the tear-away region of a rough round cylinder. The data obtained must aid in improving nuclear reactor heat exchangers. The experiments were carried out in the Re range between 2 x 10 4 and 4 x 10 6 . The roughness parameter k 5 /d (where ksub(S) is the equivalent roughness, and d is the cylinder diameter) varied in the range 0 -5 . The degree of turbulence of the incident flow Tu = 0.45%. Heat transfer values are given in relation to Re and the roughness parameter in subcritical, critical, supercritical, and transcritical flows

  16. Ten new withanolides from Physalis peruviana. (United States)

    Fang, Sheng-Tao; Liu, Ji-Kai; Li, Bo


    Ten new withanolides, including four perulactone-type withanolides, perulactones E-H (1-4), three 28-hydroxy-withanolides, withaperuvins I-K (5-7), and three other withanolides, withaperuvins L-N (8-10), together with six known compounds (11-16) were isolated from the aerial parts of Physalis peruviana. The structures of these compounds were elucidated on the basis of extensive spectroscopic analyses (1D and 2D NMR, IR, HR-MS) and chemical methods. Copyright © 2011 Elsevier Inc. All rights reserved.


    Energy Technology Data Exchange (ETDEWEB)

    Wood, Brian E. [Naval Research Laboratory, Space Science Division, Washington, DC 20375 (United States); Müller, Hans-Reinhard [Department of Physics and Astronomy, Dartmouth College, Hanover, NH 03755 (United States); Harper, Graham M., E-mail: [CASA, University of Colorado, Boulder, CO 80309-0389 (United States)


    We report on an ultraviolet spectroscopic survey of red giants observed by the Hubble Space Telescope , focusing on spectra of the Mg ii h and k lines near 2800 Å in order to study stellar chromospheric emission, winds, and astrospheric absorption. We focus on spectral types between K2 III and M5 III, a spectral type range with stars that are noncoronal, but possessing strong, chromospheric winds. We find a very tight relation between Mg ii surface flux and photospheric temperature, supporting the notion that all K2-M5 III stars are emitting at a basal flux level. Wind velocities ( V {sub w} ) are generally found to decrease with spectral type, with V {sub w} decreasing from ∼40 km s{sup −1} at K2 III to ∼20 km s{sup −1} at M5 III. We find two new detections of astrospheric absorption, for σ Pup (K5 III) and γ Eri (M1 III). This absorption signature had previously only been detected for α Tau (K5 III). For the three astrospheric detections, the temperature of the wind after the termination shock (TS) correlates with V {sub w} , but is lower than predicted by the Rankine–Hugoniot shock jump conditions, consistent with the idea that red giant TSs are radiative shocks rather than simple hydrodynamic shocks. A full hydrodynamic simulation of the γ Eri astrosphere is provided to explore this further.

  18. Mouse Genetic Models Reveal Surprising Functions of IκB Kinase Alpha in Skin Development and Skin Carcinogenesis

    Energy Technology Data Exchange (ETDEWEB)

    Xia, Xiaojun [The Methodist Hospital Research Institute, Houston, TX 77030 (United States); Park, Eunmi [Department of Radiation Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115 (United States); Fischer, Susan M. [Department of Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, Smithville, TX 78967 (United States); Hu, Yinling, E-mail: [Cancer and Inflammation Program, Center for Cancer Research, Frederick National Laboratory for Cancer Research, Frederick, MD 21701 (United States)


    Gene knockout studies unexpectedly reveal a pivotal role for IκB kinase alpha (IKKα) in mouse embryonic skin development. Skin carcinogenesis experiments show that Ikkα heterozygous mice are highly susceptible to chemical carcinogen or ultraviolet B light (UVB) induced benign and malignant skin tumors in comparison to wild-type mice. IKKα deletion mediated by keratin 5 (K5).Cre or K15.Cre in keratinocytes induces epidermal hyperplasia and spontaneous skin squamous cell carcinomas (SCCs) in Ikkα floxed mice. On the other hand, transgenic mice overexpressing IKKα in the epidermis, under the control of a truncated loricrin promoter or K5 promoter, develop normal skin and show no defects in the formation of the epidermis and other epithelial organs, and the transgenic IKKα represses chemical carcinogen or UVB induced skin carcinogenesis. Moreover, IKKα deletion mediated by a mutation, which generates a stop codon in the Ikkα gene, has been reported in a human autosomal recessive lethal syndrome. Downregulated IKKα and Ikkα mutations and deletions are found in human skin SCCs. The collective evidence not only highlights the importance of IKKα in skin development, maintaining skin homeostasis, and preventing skin carcinogenesis, but also demonstrates that mouse models are extremely valuable tools for revealing the mechanisms underlying these biological events, leading our studies from bench side to bedside.

  19. Hydrothermal synthesis and study of an inorganic-organic hybrid vanadate of a nickel(II) coordination complex with pyrazine, Ni{sub 3}(C{sub 4}H{sub 4}N{sub 2}){sub 3}(V{sub 8}O{sub 23})

    Energy Technology Data Exchange (ETDEWEB)

    Larrea, Edurne S. [Dpto. de Mineralogia y Petrologia, Facultad de Ciencia y Tecnologia, Universidad del Pais Vasco/Euskal Herriko Unibertsitatea, UPV/EHU, Apdo. 644, E-48080 Bilbao (Spain); Mesa, Jose L., E-mail: [Dpto. de Quimica Inorganica, Facultad de Ciencia y Tecnologia, Universidad del Pais Vasco/Euskal Herriko Unibertsitatea, UPV/EHU, Apdo. 644, E-48080 Bilbao (Spain); Arriortua, Maria I. [Dpto. de Mineralogia y Petrologia, Facultad de Ciencia y Tecnologia, Universidad del Pais Vasco/Euskal Herriko Unibertsitatea, UPV/EHU, Apdo. 644, E-48080 Bilbao (Spain)


    Highlights: {yields} A novel inorganic-organic hybrid vanadate of nickel(II) coordination complex with pyrazine has been synthesized hydrothermally. {yields} The thermal and spectroscopic behavior has been studied. {yields} The compound shows AFM interactions which has been fitted to a magnetic model of lineal chains. -- Abstract: The three-dimensional hybrid compound Ni{sub 3}(C{sub 4}H{sub 4}N{sub 2}){sub 3}(V{sub 8}O{sub 23}) has been synthesized by mild hydrothermal methods under autogenous pressure at 170 {sup o}C. The structure of the phase is stable until 380 {sup o}C. The removal of the pyrazine molecules from the structure induces its collapse. The IR spectrum shows the vibration modes of the pyrazine molecule and those of the [VO{sub 4}]{sup 3-} groups. A UV-visible spectrum shows the characteristic bands of the Ni(II) d{sup 8}-high-spin cation in a slightly distorted octahedral coordination. Magnetic measurements indicate the existence of antiferromagnetic interactions that can be fitted with a chain model to give g = 2.31, J/k = -5.3, and zJ'/k = -5.5.

  20. Cell cycle arrest induced by inhibitors of epigenetic modifications in maize (Zea mays) seedling leaves: characterization of the process and possible mechanisms involved. (United States)

    Wang, Pu; Zhang, Hao; Hou, Haoli; Wang, Qing; Li, Yingnan; Huang, Yan; Xie, Liangfu; Gao, Fei; He, Shibin; Li, Lijia


    Epigenetic modifications play crucial roles in the regulation of chromatin architecture and are involved in cell cycle progression, including mitosis and meiosis. To explore the relationship between epigenetic modifications and the cell cycle, we treated maize (Zea mays) seedlings with six different epigenetic modification-related inhibitors and identified the postsynthetic phase (G2 ) arrest via flow cytometry analysis. Total H4K5ac levels were significantly increased and the distribution of H3S10ph signalling was obviously changed in mitosis under various treatments. Further statistics of the cells in different periods of mitosis confirmed that the cell cycle was arrested at preprophase. Concentrations of hydrogen peroxide were relatively higher in the treated plants and the antioxidant thiourea could negate the influence of the inhibitors. Moreover, all of the treated plants displayed negative results in the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labelling (TUNEL) and γ-H2AX immunostaining assays after exposure for 3 d. Additionally, the expression level of topoisomerase genes in the treated plants was relatively lower than that in the untreated plants. These results suggest that these inhibitors of epigenetic modifications could cause preprophase arrest via reactive oxygen species formation inhibiting the expression of DNA topoisomerase genes, accompanied by changes in the H4K5ac and H3S10ph histone modifications. © 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.

  1. Response of nuclear emulsions to ionizing radiations

    International Nuclear Information System (INIS)

    Katz, R.; Pinkerton, F.E.


    Heavy ion tracks in Ilford K-2 emulsion are simulated with a computer program which makes use of the delta-ray theory of track structure, and the special assumption that the response of this emulsion to gamma-rays is 8-or-more hit. The Ilford K-series of nuclear emulsions is produced from a parent stock called K.0 emulsion, sensitized to become K.1 to K.5, and desensitized to become K-1 to K-3. Our simulations demonstrate that the emulsions K.5 through K.0 to K-1 are 1-or-more hit detectors, while K-2 is an 8-or-more hit detector. We have no data for K-3 emulsion. It would appear that emulsions of intermediate hittedness might be produced by an intermediate desensitization, to mimic or match the RBE-LET variations of biological cells, perhaps to produce a ''rem-dosimeter''. In the K-2 emulsion no developable gains are produced by stopping H, He, and Li ions. The emulsion has ''threshold-like'' properties, resembling etchable track detectors. It should prove useful in the measurement of high LET dose in a strong low LET background, as for pions or neutrons. Since it can be expected to accumulate and repair ''sub-lethal damage'', to display the ion-kill and gamma-kill inactivation modes, the grain-count and track width regimes, it may serve to model biological effects. (auth)

  2. Optical design of beam lines at the KEK-PS new experimental hall

    International Nuclear Information System (INIS)

    Tanaka, K.H.; Ieiri, M.; Noumi, H.; Minakawa, M.; Yamanoi, Y.; Kato, Y.; Ishii, H.; Suzuki, Y.; Takasaki, M.


    A new counter experimental hall [K.H. Tanaka et al., IEEE Trans. Magn. 28 (1992) 697] was designed and constructed at the KEK 12-GeV Proton Synchrotron (KEK-PS). The extracted proton beam from the KEK-PS is introduced to the new hall through the newly-prepared primary beam line, EP1, and hits two production targets in cascade. The upstream target provides secondary particles to the low momentum (0.4-0.6 GeV/c) separated beam line, K5, and the downstream target is connected to the medium momentum (0.6-2.0 GeV/c) separated beam line, K6. Several new ideas were employed in the beam optical designs of EP1, K5 and K6 in order to increase the number and the purity of the short-lived secondary particles, such as kaons and pions, under the limited energy and intensity of the primary protons provided by the KEK-PS. These new ideas are described in this paper as well as the first commissioning results. (orig.)

  3. Size-dependent structure and magnetic properties of DyMnO{sub 3} nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Cai, Xuan; Shi, Lei, E-mail:; Zhou, Shiming; Zhao, Jiyin; Guo, Yuqiao; Wang, Cailin [Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, Anhui 230026 (China)


    The structure and magnetic properties of orthorhombic DyMnO{sub 3} nanoparticles with different particle sizes are investigated in this paper. With decreasing particle size, all the lattice parameters a, b, and c gradually decrease, whereas the orthorhombic distortion increases. Magnetic measurements reveal that the antiferromagnetic interaction of Mn ions is weakened due to the decrease in Mn-O-Mn bond angle. Above a critical field H*, DyMnO{sub 3} undergoes a field-induced metamagnetic transition at 4 K, which is related to the spin reversal of Dy moments. The critical field H* increases monotonically with size reduction, indicating an enhancement of the antiferromagnetic interaction of Dy ions due to the decreased distance between rare earth ions. The magnetization at 4 K and 5 T, i.e., M(4 K, 5 T) shows a non-monotonic variation with particle size d, i.e., M(4 K, 5 T) initially increases with size reduction but decreases again for d < 68 nm. A modified core-shell model, in which the ferromagnetic ordering (Dy magnetic structure) and antiferromagnetic ordering (Mn magnetic structure) coexist in the core, is proposed to explain this behavior.

  4. Briquettes of rice husk, polyethylene terephthalate (PET), and dried leaves as implementation of wastes recycling (United States)

    Hariyanto, Sucipto; Usman, Mohammad Nurdianfajar; Citrasari, Nita


    This research aim is to determine the best briquettes as implementation of wastes recycle based on scoring method, main component composition, compressive strength, caloric value, water content, vollatile content, and ash content, also the suitability with SNI 01-6235-2000. Main component that used are rice husk, 2mm and 6 mm PET, and dried leaves. Composition variation in this research are marked as K1, K2, K3, K4, and K5 with 2 mm PET plastic and K1, K2, K3, K4, and K5 with 6 mm PET plastic. The total weight of the briquettes is 100 g and divided into 90% main components and 10% tapioca as binder. The compressive strength, caloric value, water content, vollatile content, and ash content were tested according to ASTM D 5865-04, ASTM D 3173-03, ASTM D 3175-02, ASTM D 3174-02. The tested results were used to determine the best briquette by scoring method, and the chosen briquettes is K2 with 6 mm PET plastic. The composition is 70% rice husk, 20% 6 mm PET plastic, and 10% dried leaves with the compressive strength, caloric value, water content, vollatile content, and ash content value is 51,55 kg/cm2; 5123 kal/g; 3,049%; 31,823%, dan 12,869%. The suitable value that meet the criteria according to SNI 01-6235-2000 is compressive strength, caloric value, water content, and ash content.

  5. Geometric Simulation Approach for Grading and Assessing the Thermostability of CALBs

    Directory of Open Access Journals (Sweden)

    B. Senthilkumar


    Full Text Available Candida antarctica lipase B (CALB is a known stable and highly active enzyme used widely in biodiesel synthesis. In this work, the stability of native (4K6G and mutant (4K5Q CALB was studied through various structural parameters using conformational sampling approach. The contours of polar surface area and surface area of mutant CALB were 11357.67 Å2 and 30007.4 Å2, respectively, showing an enhanced stability compared to native CALB with a statistically significant P value of < 0.0001. Moreover, simulated thermal denaturation of CALB, a process involving dilution of hydrogen bond, significantly shielded against different intervals of energy application in mutant CALB revealing its augmentation of structural rigidity against native CALB. Finally, computational docking analysis showed an increase in the binding affinity of CALB and its substrate (triglyceride in mutant CALB with Atomic Contact Energy (ACE of −91.23 kcal/mol compared to native CALB (ACE of −70.3 kcal/mol. The computational observations proposed that the use of mutant CALB (4K5Q could serve as a best template for production of biodiesel in the future. Additionally, it can also be used as a template to identify efficient thermostable lipases through further mutations.

  6. Depression- and anxiety-like behaviour is related to BDNF/TrkB signalling in a mouse model of psoriasis. (United States)

    JiaWen, W; Hong, S; ShengXiang, X; Jing, L


    The prevalence of anxiety and depression is significantly higher in individuals with psoriasis than in the general population. Clinical data also show that anti-anxiety and antidepression drugs can reduce skin lesions in patients with psoriasis, but the actual mechanism is still poorly understood. To investigate whether brain-derived neurotrophic factor (BDNF) and tropomyosin receptor kinase B (TrKB) signalling plays a role in the mechanism underlying psoriasis with depression and anxiety behaviours. Expression of BDNF and tropomyosin receptor kinase B (TrKB) in the K5.Stat3C mouse, an animal model of psoriasis, were investigated by reverse transcription PCR and Western blotting. Anxiety-like behaviours in the elevated-plus maze test and changes in BDNF/TrkB that have been implicated in depression and anxiety behaviours were measured. Skin lesions induced by 12-O-tetradecanoyl phorbol-13-acetate (TPA) were also measured when the mice were administered fluoxetine and K252a, an antagonist of TrkB. The antidepression and anti-anxiety drug fluoxetine reduced TPA-induced skin lesions and increased expression of BDNF and TrkB in K5.Stat3C mice. More importantly, the effects of fluoxetine were reversed by the TrkB antagonist K252a. BDNF/TrkB signalling participates in the pathological mechanism of depression and anxiety behaviours in psoriasis. Our findings provide a new therapeutic strategy for the treatment of skin lesions in psoriasis. © 2018 British Association of Dermatologists.

  7. A Review of the Multilevel Slug Test for Characterizing Aquifer Heterogeneity

    Directory of Open Access Journals (Sweden)

    Chia-Shyun Chen


    Full Text Available All aquifers are heterogeneous to a certain degree. The spatial distribution of hydraulic conductivity K(x, y, z, or aquifer heterogeneity, significantly influences the groundwater flow movement and associated solute transport. Of particular importance in designing an in-situ remediation plan is a knowledge of low-K layers because they are less accessible to remedial agents and form a bottleneck in remediation. The characterization of aquifer heterogeneity is essential to the solution of many practical and scientific groundwater problems. This article reviews the field technique using the multilevel slug test (MLST, which determines a series of K estimates at depths of interest in a well by making use of a double-packer system. The K(z obtained manifests the vertical variation of hydraulic conductivity in the vicinity of the test well, and the combination of K(z from different wells gives rise to a three-dimensional description of K(x, y, z. The MLST response is rather sensitive to hydraulic conductivity variation; e.g., it is oscillatory for highly permeable conditions (K > 5 × 10-4 m s-1 and a nonoscillatory for K < 5 × 10-4 m s-1. In this article we discuss the instrumentation of the double-packer system, the implementation of the depth-specific slug test, the data analysis methods for a spectrum of response characteristics usually observed in the field, and field applications of the MLST.

  8. Constants of acid‒base equilibria in an aqueous amikacin aminoglycoside solution at 298 K (United States)

    Alekseev, V. G.; Markova, E. V.


    The acid dissociation constants of form p K 1 = 7.34 ± 0.01, p K 2 = 7.84 ± 0.01, p K 3 = 8.77 ± 0.01, p K 4 = 9.49 ± 0.01, and p K 5 = 10.70 ± 0.02 of cationic amikacin are determined by pH-metric titration at 25°C against the background of 0.1 mol/L KNO3. K 1, K 2, K 3, and K 4 correspond to the dissociation of protons coordinated to amino groups, while K 5 characterizes the dissociation of the hydroxyl hydrogen atom, testifying to the amphoteric character of amikacin molecules. Applying density functional theory (DFT) with the B3LYP hybrid functional and the 6-311G**++ basis set, the partial charges on the atoms of an amikacin molecule are calculated. It is concluded that the dissociation of H(55)hydrogen atom occurs with a greatest partial charge of +0.53631.

  9. Improved high temperature superconductor materials for wind turbine generators

    Energy Technology Data Exchange (ETDEWEB)

    Mozhaev, P.B.; Khoryushin, A.V.; Mozhaeva, J.E.; Bindslev Hansen, J.; Jacobsen, Claus S. (Technical Univ. of Denmark (DTU). Physics Dept., Kgs. Lyngby (Denmark)); Andersen, Niels H.; Grivel, J.-C. (Technical Univ. of Denmark (DTU). Risoe National Lab. for Sustainable Energy, Roskilde (Denmark))


    Effects of yttria addition on the structural and electrical properties of the YBCO thin films are studied. The films were deposited on (LaAlO{sub 3}){sub .3}-(Sr{sub 2}AlTaO{sub 8}){sub .7} substrates by pulsed laser ablation from targets with different elemental composition. The contents of elements in the film depend mainly on the yttrium content in the target. An increase of yttrium content leads to formation of a porous film with significant improvement of current-carrying capabilities (critical current density reaches 35 kA/cm2 at 77 K, 5 T, and exceeds 2 MA/cm2 at 50 K, 5 T). The Y-enriched YBCO film remains c-oriented up to 600 nm thickness with no suppression of the critical current density in the film. Yttria decoration of the substrate surface prior to deposition resulted in formation of YBCO films with low strain and high crystal perfection. In contrast to the Y-enriched YBCO films, the films on yttria layers are dense. At temperatures of 77 K and above the YBCO films on yttria-decorated substrates exhibit critical current densities comparable to or better than that of the Y-enriched films. (Author)

  10. A new perspective in the estimation of postmortem interval (PMI) based on vitreous. (United States)

    Muñoz, J I; Suárez-Peñaranda, J M; Otero, X L; Rodríguez-Calvo, M S; Costas, E; Miguéns, X; Concheiro, L


    The relation between the potassium concentration in the vitreous humor, [K+], and the postmortem interval has been studied by several authors. Many formulae are available and they are based on a correlation test and linear regression using the PMI as the independent variable and [K+] as the dependent variable. The estimation of the confidence interval is based on this formulation. However, in forensic work, it is necessary to use [K+] as the independent variable to estimate the PMI. Although all authors have obtained the PMI by direct use of these formulae, it is, nevertheless, an inexact approach, which leads to false estimations. What is required is to change the variables, obtaining a new equation in which [K+] is considered as the independent variable and the PMI as the dependent. The regression line obtained from our data is [K+] = 5.35 + 0.22 PMI, by changing the variables we get PMI = 2.58[K+] - 9.30. When only nonhospital deaths are considered, the results are considerably improved. In this case, we get [K+] = 5.60 + 0.17 PMI and, consequently, PMI = 3.92[K+] - 19.04.

  11. Thankful for the little things: A meta-analysis of gratitude interventions. (United States)

    Davis, Don E; Choe, Elise; Meyers, Joel; Wade, Nathaniel; Varjas, Kristen; Gifford, Allison; Quinn, Amy; Hook, Joshua N; Van Tongeren, Daryl R; Griffin, Brandon J; Worthington, Everett L


    A recent qualitative review by Wood, Froh, and Geraghty (2010) cast doubt on the efficacy of gratitude interventions, suggesting the need to carefully attend to the quality of comparison groups. Accordingly, in a series of meta-analyses, we evaluate the efficacy of gratitude interventions (ks = 4-18; Ns = 395-1,755) relative to a measurement-only control or an alternative-activity condition across 3 outcomes (i.e., gratitude, anxiety, psychological well-being). Gratitude interventions outperformed a measurement-only control on measures of psychological well-being (d = .31, 95% confidence interval [CI = .04, .58]; k = 5) but not gratitude (d = .20; 95% CI [-.04, .44]; k = 4). Gratitude interventions outperformed an alternative-activity condition on measures of gratitude (d = .46, 95% CI [.27, .64]; k = 15) and psychological well-being (d = .17, 95% CI [.09, .24]; k = 20) but not anxiety (d = .11, 95% CI [-.08, .31]; k = 5). More-detailed subdivision was possible on studies with outcomes assessing psychological well-being. Among these, gratitude interventions outperformed an activity-matched comparison (d = .14; 95% CI [.01, .27]; k = 18). Gratitude interventions performed as well as, but not better than, a psychologically active comparison (d = -.03, 95% CI [-.13, .07]; k = 9). On the basis of these findings, we summarize the current state of the literature and make suggestions for future applied research on gratitude. (PsycINFO Database Record (c) 2016 APA, all rights reserved).

  12. Mouse Genetic Models Reveal Surprising Functions of IκB Kinase Alpha in Skin Development and Skin Carcinogenesis

    International Nuclear Information System (INIS)

    Xia, Xiaojun; Park, Eunmi; Fischer, Susan M.; Hu, Yinling


    Gene knockout studies unexpectedly reveal a pivotal role for IκB kinase alpha (IKKα) in mouse embryonic skin development. Skin carcinogenesis experiments show that Ikkα heterozygous mice are highly susceptible to chemical carcinogen or ultraviolet B light (UVB) induced benign and malignant skin tumors in comparison to wild-type mice. IKKα deletion mediated by keratin 5 (K5).Cre or K15.Cre in keratinocytes induces epidermal hyperplasia and spontaneous skin squamous cell carcinomas (SCCs) in Ikkα floxed mice. On the other hand, transgenic mice overexpressing IKKα in the epidermis, under the control of a truncated loricrin promoter or K5 promoter, develop normal skin and show no defects in the formation of the epidermis and other epithelial organs, and the transgenic IKKα represses chemical carcinogen or UVB induced skin carcinogenesis. Moreover, IKKα deletion mediated by a mutation, which generates a stop codon in the Ikkα gene, has been reported in a human autosomal recessive lethal syndrome. Downregulated IKKα and Ikkα mutations and deletions are found in human skin SCCs. The collective evidence not only highlights the importance of IKKα in skin development, maintaining skin homeostasis, and preventing skin carcinogenesis, but also demonstrates that mouse models are extremely valuable tools for revealing the mechanisms underlying these biological events, leading our studies from bench side to bedside

  13. Rational Design of Cyclic Antimicrobial Peptides Based on BPC194 and BPC198

    Directory of Open Access Journals (Sweden)

    Anna D. Cirac


    Full Text Available A strategy for the design of antimicrobial cyclic peptides derived from the lead compounds c(KKLKKFKKLQ (BPC194 and c(KLKKKFKKLQ (BPC198 is reported. First, the secondary β-structure of BPC194 and BPC198 was analyzed by carrying out molecular dynamics (MD simulations. Then, based on the sequence pattern and the β-structure of BPC194 or BPC198, fifteen analogues were designed and synthesized on solid-phase. The best peptides (BPC490, BPC918, and BPC924 showed minimum inhibitory concentration (MIC values <6.2 μM against Pseudomonas syringae pv. syringae and Xanthomonas axonopodis pv. vesicatoria, and an MIC value of 12.5 to 25 μM against Erwinia amylovora, being as active as BPC194 and BPC198. Interestingly, these three analogues followed the structural pattern defined from the MD simulations of the parent peptides. Thus, BPC490 maintained the parallel alignment of the hydrophilic pairs K1–K8, K2–K7, and K4–K5, whereas BPC918 and BPC924 included the two hydrophilic interactions K3–Q10 and K5–K8. In short, MD simulations have proved to be very useful for ascertaining the structural features of cyclic peptides that are crucial for their biological activity. Such approaches could be further employed for the development of new antibacterial cyclic peptides.

  14. Behavior of lambda 2800 Mg II in stellar spectra

    International Nuclear Information System (INIS)

    Gurzadyan, G.A.


    The results of measurements of the equivalent widths of the resonance doublet of ionized magnesium lambda 2800 Mg II in the spectra of 51 relatively faint stars, up to 10/sup m/, of the spectral classes B1-K5 are presented. The observed material has been obtained by means of the space observatory ''Orion-2''. Some regularities in the behavior of lambda 2800 Mg II in stellar spectra have been revealed: wide and deep depression of the continuous spectra at 2800 A in F-G type stars; the presence of the doublet lambda 2800 Mg II in the form of weak emission in the spectra of cold stars (K2-K5); the presence both of the multiplet lambda 3080 Ti II and the doublet lambda 2800 Mg II simultaneously either in emission--in the late-type stars--or in absorption in earlier types; the existence of combined profiles of lambda 2800 Mg II, i.e., a wide absorption line with a weak emission in the center, in stars of the transitional class (G5-K0), etc. A well-defined empirical relationship between the equivalent width of lambda 2800 Mg II and the spectral class of the star has been established (Fig. 8). (U.S.)

  15. The development of character education by improving student motivation in science

    International Nuclear Information System (INIS)

    Baykent, D.


    The goal of this study is, first to provide students with better comprehension skills in Science field by encouraging them to raise an interest in Science by applying convenient teaching methods, systems, and tools. Therefore, to use the positive environment developed through motivation consciously and build desired and expected behaviours in the society by means of character education. Appropriate character education is to be carried out by relating Science topics to the current daily life.The field of this study was V.K.V. Koc School. Applications were occurred with 666 students from K1 to K5 levels. Measurements and evaluation field was limited with K4 and K5 levels, who joined the Science Laboratory Activities periodically. The study has been applied between 2000 autumn term and 2002 spring term. Additionally, there is a comparative adaptation on the study of 2002-2003 education year, which was adapted to the American K8 level students at Lake Stevens Middle School, Seattle, Washington, USA

  16. Size-dependent structure and magnetic properties of DyMnO3 nanoparticles

    International Nuclear Information System (INIS)

    Cai, Xuan; Shi, Lei; Zhou, Shiming; Zhao, Jiyin; Guo, Yuqiao; Wang, Cailin


    The structure and magnetic properties of orthorhombic DyMnO 3 nanoparticles with different particle sizes are investigated in this paper. With decreasing particle size, all the lattice parameters a, b, and c gradually decrease, whereas the orthorhombic distortion increases. Magnetic measurements reveal that the antiferromagnetic interaction of Mn ions is weakened due to the decrease in Mn-O-Mn bond angle. Above a critical field H*, DyMnO 3 undergoes a field-induced metamagnetic transition at 4 K, which is related to the spin reversal of Dy moments. The critical field H* increases monotonically with size reduction, indicating an enhancement of the antiferromagnetic interaction of Dy ions due to the decreased distance between rare earth ions. The magnetization at 4 K and 5 T, i.e., M(4 K, 5 T) shows a non-monotonic variation with particle size d, i.e., M(4 K, 5 T) initially increases with size reduction but decreases again for d < 68 nm. A modified core-shell model, in which the ferromagnetic ordering (Dy magnetic structure) and antiferromagnetic ordering (Mn magnetic structure) coexist in the core, is proposed to explain this behavior.

  17. The prostaglandin EP1 receptor potentiates kainate receptor activation via a protein kinase C pathway and exacerbates status epilepticus (United States)

    Rojas, Asheebo; Gueorguieva, Paoula; Lelutiu, Nadia; Quan, Yi; Shaw, Renee; Dingledine, Raymond


    Prostaglandin E2 (PGE2) regulates membrane excitability, synaptic transmission, plasticity, and neuronal survival. The consequences of PGE2 release following seizures has been the subject of much study. Here we demonstrate that the prostaglandin E2 receptor 1 (EP1, or Ptger1) modulates native kainate receptors, a family of ionotropic glutamate receptors widely expressed throughout the central nervous system. Global ablation of the EP1 gene in mice (EP1-KO) had no effect on seizure threshold after kainate injection but reduced the likelihood to enter status epilepticus. EP1-KO mice that did experience typical status epilepticus had reduced hippocampal neurodegeneration and a blunted inflammatory response. Further studies with native prostanoid and kainate receptors in cultured cortical neurons, as well as with recombinant prostanoid and kainate receptors expressed in Xenopus oocytes, demonstrated that EP1 receptor activation potentiates heteromeric but not homomeric kainate receptors via a second messenger cascade involving phospholipase C, calcium and protein kinase C. Three critical GluK5 C-terminal serines underlie the potentiation of the GluK2/GluK5 receptor by EP1 activation. Taken together, these results indicate that EP1 receptor activation during seizures, through a protein kinase C pathway, increases the probability of kainic acid induced status epilepticus, and independently promotes hippocampal neurodegeneration and a broad inflammatory response. PMID:24952362

  18. Macroscopic rate equation modeling of trapping/detrapping of hydrogen isotopes in tungsten materials

    Energy Technology Data Exchange (ETDEWEB)

    Hodille, E.A., E-mail: [CEA, IRFM, F-13108 Saint Paul lez Durance (France); Bonnin, X. [LSPM-CNRS, Université Paris 13, Sorbonne Paris Cité, F-93430 Villetaneuse (France); Bisson, R.; Angot, T. [Aix-Marseille Université, PIIM, CNRS, UMR 7345, 13397 Marseille (France); Becquart, C.S. [Université Lille I, UMET, UMR 8207, 59655 Villeneuve d’Ascq cédex France (France); Layet, J.M. [Aix-Marseille Université, PIIM, CNRS, UMR 7345, 13397 Marseille (France); Grisolia, C. [CEA, IRFM, F-13108 Saint Paul lez Durance (France)


    Relevant parameters for trapping of Hydrogen Isotopes (HIs) in polycrystalline tungsten are determined with the MHIMS code (Migration of Hydrogen Isotopes in MaterialS) which is used to reproduce Thermal Desorption Spectrometry experiments. Three types of traps are found: two intrinsic traps (detrapping energy of 0.87 eV and 1.00 eV) and one extrinsic trap created by ion irradiation (detrapping energy of 1.50 eV). Then MHIMS is used to simulate HIs retention at different fluences and different implantation temperatures. Simulation results agree well with experimental data. It is shown that at 300 K the retention is limited by diffusion in the bulk. For implantation temperatures above 500 K, the retention is limited by trap creation processes. Above 600 K, the retention drops by two orders of magnitude as compared to the retention at 300 K. With the determined detrapping energies, HIs outgassing at room temperature is predicted. After ions implantation at 300 K, 45% of the initial retention is lost to vacuum in 300 000 s while during this time the remaining trapped HIs diffuse twice as deep into the bulk. - Highlights: • Code development to solve numerically the model equations of diffusion and trapping of hydrogen in metals. • Parametrization of the model trapping parameters (detrapping energies and density): fitting of experimental TDS spectrum. • Confrontation model/experiment: evolution of retention with fluence and implantation temperature. • Investigation of period of rest between implantation and TDS on retention and depth profile.

  19. Adsorption of reactive dyes from aqueous solutions by fly ash: Kinetic and equilibrium studies

    International Nuclear Information System (INIS)

    Dizge, N.; Aydiner, C.; Demirbas, E.; Kobya, M.; Kara, S.


    Adsorption kinetic and equilibrium studies of three reactive dyes namely, Remazol Brillant Blue (RB), Remazol Red 133 (RR) and Rifacion Yellow HED (RY) from aqueous solutions at various initial dye concentration (100-500 mg/l), pH (2-8), particle size (45-112.5 μm) and temperature (293-323 K) on fly ash (FA) were studied in a batch mode operation. The adsorbent was characterized with using several methods such as SEM, XRD and FTIR. Adsorption of RB reactive dye was found to be pH dependent but both RR and RY reactive dyes were not. The result showed that the amount adsorbed of the reactive dyes increased with increasing initial dye concentration and contact time. Batch kinetic data from experimental investigations on the removal of reactive dyes from aqueous solutions using FA have been well described by external mass transfer and intraparticle diffusion models. It was found that external mass transfer and intraparticle diffusion had rate limiting affects on the removal process. This was attributed to the relatively simple macropore structure of FA particles. The adsorption data fitted well with Langmuir and Freundlich isotherm models. The optimum conditions for removal of the reactive dyes were 100 mg/l initial dye concentration, 0.6 g/100 ml adsorbent dose, temperature of 293 K, 45 μm particle size, pH 6 and agitation speed of 250 rpm, respectively. The values of Langmuir and Freundlich constants were found to increase with increasing temperature in the range 135-180 and 15-34 mg/g for RB, 47-86 and 1.9-3.7 mg/g for RR and 37-61 and 3.0-3.6 mg/g for RY reactive dyes, respectively. Different thermodynamic parameters viz., changes in standard free energy, enthalpy and entropy were evaluated and it was found that the reaction was spontaneous and endothermic in nature

  20. Assessment of natural radioactivity levels in rocks and their relationships with the geological structure of Johor state, Malaysia. (United States)

    Alnour, I A; Wagiran, H; Ibrahim, N; Hamzah, S; Elias, M S; Laili, Z; Omar, M


    The distribution of natural radionuclides ((238)U, (232)Th and (40)K) and their radiological hazard effect in rocks collected from the state of Johor, Malaysia were determined by gamma spectroscopy using a high-purity germanium detector. The highest values of (238)U, (232)Th and (40)K activity concentrations (67±6, 85±7 and 722±18 Bg kg(-1), respectively) were observed in the granite rock. The lowest concentrations of (238)U and (232)Th (2±0.1 Bq kg(-1) for (238)U and 2±0.1 Bq kg(-1) for (232)Th) were observed in gabbro rock. The lowest concentration of (40)K (45±2 Bq kg(-1)) was detected in sandstone. The radium equivalent activity concentrations for all rock samples investigated were lower than the internationally accepted value of 370 Bq kg(-1). The highest value of radium equivalent in the present study (239±17 Bq kg(-1)) was recorded in the area of granite belonging to an acid intrusive rock geological structure. The absorbed dose rate was found to range from 4 to 112 nGy h(-1). The effective dose ranged from 5 to 138 μSv h(-1). The internal and external hazard index values were given in results lower than unity. The purpose of this study is to provide information related to radioactivity background levels and the effects of radiation on residents in the study area under investigation. Moreover, the relationships between the radioactivity levels in the rocks within the geological structure of the studied area are discussed.

  1. A meta-analysis of experimental studies of diversion programs for juvenile offenders. (United States)

    Schwalbe, Craig S; Gearing, Robin E; MacKenzie, Michael J; Brewer, Kathryne B; Ibrahim, Rawan


    Research to establish an evidence-base for the treatment of conduct problems and delinquency in adolescence is well established; however, an evidence-base for interventions with offenders who are diverted from the juvenile justice system has yet to be synthesized. The purpose of this study was to conduct a meta-analysis of experimental studies testing juvenile diversion programs and to examine the moderating effect of program type and implementation quality. A literature search using PsycINFO, Web of Science, and the National Criminal Justice Reference Service data-bases and research institute websites yielded 28 eligible studies involving 57 experimental comparisons and 19,301 youths. Recidivism was the most common outcome reported across all studies. Overall, the effect of diversion programs on recidivism was non-significant (k=45, OR=0.83, 95%CI=0.43-1.58). Of the five program types identified, including case management (k=18, OR=0.78), individual treatment (k=11, OR=0.83), family treatment (k=4, OR=0.57), youth court (k=6, OR=0.93), and restorative justice (k=6, OR=0.87), only family treatment led to a statistically significant reduction in recidivism. Restorative justice studies that were implemented with active involvement of researchers led to statistically significant reductions in recidivism (k=3, OR=0.69). Other outcomes, including frequency of offending, truancy, and psycho-social problems were reported infrequently and were not subjected to meta-analysis. High levels of heterogeneity characterize diversion research. Results of this study recommend against implementation of programs limited to case management and highlight the promise of family interventions and restorative justice. Copyright © 2011 Elsevier Ltd. All rights reserved.

  2. Austenite-martensite transformation in electrodeposited Fe70Pd30 NWs: a step towards making bio-nano-actuators tested on in vivo systems (United States)

    Zuzek Rozman, K.; Pecko, D.; Trafela, S.; Samardzija, Z.; Spreitzer, M.; Jaglicic, Z.; Nadrah, P.; Zorko, M.; Bele, M.; Tisler, T.; Pintar, A.; Sturm, S.; Kostevsek, N.


    Fe69±3Pd31±3 nanowires (NWs) with lengths of a few microns and diameters of 200 nm were synthesized via template-assisted pulsed electrodeposition into alumina-based templates. The as-deposited Fe69±3Pd31±3 NWs exhibited α-Fe (bcc-solid solution of Fe, Pd) nanocrystalline structure as seen from the x-ray diffraction (XRD), that got confirmed by transmission electron microscopy (TEM) with some larger grains up 50 nm observed. Annealing of the as-deposited Fe69±3Pd31±3 NWs at 1173 K/45 min was followed by quenching in ice water and resulted in a transformation to the fcc crystal structure (XRD) with grain sizes up to 200 nm (TEM). To induce the austenite-to-martensite, i.e., fcc-to-fct phase transformation the fcc Fe69±3Pd31±3 NWs were cooled to 73 K. The XRD showed the disappearance of the (200) fcc reflection (at room temperature) and the appearance of the (200) fct reflection (at 73 K), confirming the fcc-to-fct transformation took place. The magnetic measurements revealed that the fcc Fe69±3Pd31±3 NWs measured at low temperatures (50 K) had a larger coercivity than at room temperature, which suggests the fct phase was present in the undercooled state, exhibiting a larger magnetocrystalline anisotropy than the fcc phase present at room temperature. As part of our interest in magnetic-shape-memory actuators, the as-deposited Fe69±3Pd31±3 NWs were tested for toxicity on zebrafish. In vivo tests showed no acute lethal or sub-lethal effects, which implies that the Fe69±3Pd31±3 NWs have the potential to be used as nano-actuators in biomedical applications.


    Directory of Open Access Journals (Sweden)

    Mutiara Ayu Banjarsari


    Full Text Available The data pile on a database of academic information systems at Computer Science Program of Mathematic and Natural Science Faculty of Lambung Mangkurat University is not fully utilized, although it can provide new information that has not been known before. Data mining techniques can be used to predict the timely graduation of students. The k-Nearest Nieghbor, a method to classify objects based on training data located closest to the object, was used in this study. Selection of the value of k in kNN algorithm became important because it would affect the performance of the algorithm kNN, therefore it was necessary to know how the value of k and the level of accuracy. The k-Fold Cross Validation method and Accuracy Test was used to determine the value of k-Optimal. The result showed that the value of k = 5 was defined as k-Optimal which was then be applied in the kNN algorithm for prediction of timely graduation of students based on the Grade Point Average up to 4th semester. Keywords: kNN, k-Optimal, Classification, Data mining, k-Fold Cross Validation method Tumpukan data pada database sistem informasi akademik Program Studi Ilmu Komputer FMIPA Unlam belum dimanfaatkan secara maksimal, padahal dari data tersebut dapat memberikan sebuah informasi baru yang belum diketahui sebelumnya. Teknik data mining dapat digunakan untuk memprediksi kelulusan tepat waktu mahasiswa. Penelitian menggunakan metode k-Nearest Nieghbor yang merupakan sebuah metode untuk melakukan klasifikasi terhadap objek berdasarkan data training yang jaraknya paling dekat dengan objek tersebut. Pemilihan nilai k pada algoritma kNN menjadi hal yang penting karena akan mempengaruhi kinerja dari algoritma kNN, oleh karena itu perlu diketahui berapa nilai k dan tingkat akurasinya. Metode k-Fold Cross Validation dan Uji Akurasi digunakan untuk mengetahui nilai k-Optimal. Hasil yang didapat adalah nilai k=5 dengan tingkat akurasi sebesar 80.00% yang ditetapkan sebagai k-Optimal. Nilai k

  4. Methodology for the preliminary design of high performance schools in hot and humid climates (United States)

    Im, Piljae

    A methodology to develop an easy-to-use toolkit for the preliminary design of high performance schools in hot and humid climates was presented. The toolkit proposed in this research will allow decision makers without simulation knowledge easily to evaluate accurately energy efficient measures for K-5 schools, which would contribute to the accelerated dissemination of energy efficient design. For the development of the toolkit, first, a survey was performed to identify high performance measures available today being implemented in new K-5 school buildings. Then an existing case-study school building in a hot and humid climate was selected and analyzed to understand the energy use pattern in a school building and to be used in developing a calibrated simulation. Based on the information from the previous step, an as-built and calibrated simulation was then developed. To accomplish this, five calibration steps were performed to match the simulation results with the measured energy use. The five steps include: (1) Using an actual 2006 weather file with measured solar radiation, (2) Modifying lighting & equipment schedule using ASHRAE's RP-1093 methods, (3) Using actual equipment performance curves (i.e., scroll chiller), (4) Using the Winkelmann's method for the underground floor heat transfer, and (5) Modifying the HVAC and room setpoint temperature based on the measured field data. Next, the calibrated simulation of the case-study K-5 school was compared to an ASHRAE Standard 90.1-1999 code-compliant school. In the next step, the energy savings potentials from the application of several high performance measures to an equivalent ASHRAE Standard 90.1-1999 code-compliant school. The high performance measures applied included the recommendations from the ASHRAE Advanced Energy Design Guides (AEDG) for K-12 and other high performance measures from the literature review as well as a daylighting strategy and solar PV and thermal systems. The results show that the net

  5. Repair of tracheal epithelium by basal cells after chlorine-induced injury

    Directory of Open Access Journals (Sweden)

    Musah Sadiatu


    Full Text Available Abstract Background Chlorine is a widely used toxic compound that is considered a chemical threat agent. Chlorine inhalation injures airway epithelial cells, leading to pulmonary abnormalities. Efficient repair of injured epithelium is necessary to restore normal lung structure and function. The objective of the current study was to characterize repair of the tracheal epithelium after acute chlorine injury. Methods C57BL/6 mice were exposed to chlorine and injected with 5-ethynyl-2′-deoxyuridine (EdU to label proliferating cells prior to sacrifice and collection of tracheas on days 2, 4, 7, and 10 after exposure. Airway repair and restoration of a differentiated epithelium were examined by co-localization of EdU labeling with markers for the three major tracheal epithelial cell types [keratin 5 (K5 and keratin 14 (K14 for basal cells, Clara cell secretory protein (CCSP for Clara cells, and acetylated tubulin (AcTub for ciliated cells]. Morphometric analysis was used to measure proliferation and restoration of a pseudostratified epithelium. Results Epithelial repair was fastest and most extensive in proximal trachea compared with middle and distal trachea. In unexposed mice, cell proliferation was minimal, all basal cells expressed K5, and K14-expressing basal cells were absent from most sections. Chlorine exposure resulted in the sloughing of Clara and ciliated cells from the tracheal epithelium. Two to four days after chlorine exposure, cell proliferation occurred in K5- and K14-expressing basal cells, and the number of K14 cells was dramatically increased. In the period of peak cell proliferation, few if any ciliated or Clara cells were detected in repairing trachea. Expression of ciliated and Clara cell markers was detected at later times (days 7–10, but cell proliferation was not detected in areas in which these differentiated markers were re-expressed. Fibrotic lesions were observed at days 7–10 primarily in distal trachea. Conclusion

  6. Effect of current sheets on the solar wind magnetic field power spectrum from the Ulysses observation: from Kraichnan to Kolmogorov scaling. (United States)

    Li, G; Miao, B; Hu, Q; Qin, G


    The MHD turbulence theory developed by Iroshnikov and Kraichnan predicts a k(-1.5) power spectrum. Solar wind observations, however, often show a k(-5/3) Kolmogorov scaling. Based on 3 years worth of Ulysses magnetic field data where over 28,000 current sheets are identified, we propose that the current sheet is the cause of the Kolmogorov scaling. We show that for 5 longest current-sheet-free periods the magnetic field power spectra are all described by the Iroshnikov-Kraichnan scaling. In comparison, for 5 periods that have the most number of current sheets, the power spectra all exhibit Kolmogorov scaling. The implication of our results is discussed.

  7. Eigengaps for Hub-Dominant Matrices (United States)


    frame ( ETF ) if it satisfies two conditions: (i) For some nonnegative , we have jhvk, vjij¼ when 1 k5jN and (ii) PN k¼1 vkv t k ¼ Ns Id, when Id...and ETF lead to the following results. 1238 L. Shen and B.W. Suter D ow nl oa de d by [ L ix in S he n] a t 0 4: 58 0 8 Ju ly 2 01 1 13...1 of A forms an ETF , then (i) kamk 1; (ii) ¼ (m 2) þ 1, where is the constant in (23) and ¼ jhai, ajij for all 1 i5jm 1 and (iii) 1m2

  8. Chromospherically active stars. 13: HD 30957: A double lined K dwarf binary (United States)

    Fekel, Francis C.; Dadonas, Virgilijus; Sperauskas, Julius; Vaccaro, Todd R.; Patterson, L. Ronald


    HD 30957 is a double-lined spectroscopic binary with a period of 44.395 days and a modest eccentricity of 0.09. The spectral types of the components are K2-3 V and K5 V. The measured v sin i for both components is less than or equal to 3 km/s and the orbital inclination is estimated to be 69 deg. The system is relatively nearby with a parallax of 0.025 sec or a distance of 40 pc. Space motions of the system indicate that it does not belong to any of the known moving groups. Absolute surface fluxes of the Ca II H and K lines have been recomputed and indicate only modest chromospheric activity. If the stars are rotating pseudosynchronously, the lack of light variability is consistent with the value of the critical Rossby number for starspot activity.

  9. IRAS observations of chromospherically active dwarf stars (United States)

    Tsikoudi, Vassiliki


    Far-infrared observations of chromospherically active, spotted, and plage stars in the dF7-dk7 spectral range are examined. Most (75 percent) of the stars have detectable 12-micron fluxes, and 50 percent of them have 25-micron emission. The 12-micron luminosity, L(12), is found to be in the range of 1.5-13 x 10 to the 30th ergs/s and to comprise only 0.2-0.5 percent of the star's total luminosity, L(bol). The present work extends to earlier spectral types and higher stellar luminosities the L(12) vs L(bol) relationship noted previously for late-type active dwarfs (K5-M5).

  10. IRAS observations of chromospherically active dwarf stars

    Energy Technology Data Exchange (ETDEWEB)

    Tsikoudi, V. (Ioannina Univ. (Greece))


    Far-infrared observations of chromospherically active, spotted, and plage stars in the dF7-dk7 spectral range are examined. Most (75 percent) of the stars have detectable 12-micron fluxes, and 50 percent of them have 25-micron emission. The 12-micron luminosity, L(12), is found to be in the range of 1.5-13 x 10 to the 30th ergs/s and to comprise only 0.2-0.5 percent of the star's total luminosity, L(bol). The present work extends to earlier spectral types and higher stellar luminosities the L(12) vs L(bol) relationship noted previously for late-type active dwarfs (K5-M5). 17 refs.

  11. Antioxidative Activity of Tobacco Leaf Protein Hydrolysates

    Directory of Open Access Journals (Sweden)

    Guohua Rao


    Full Text Available Discarded tobacco leaf protein hydrolysate (DTLPH was prepared by enzymatic hydrolysis using papain and then separated using ultrafiltration (UF membranes with molecular mass cut-off (MMCO of 10, 5, 3 and 1 kDa. Four permeate fractions including 10-K, 5-K, 3-K and 1-K (the permeate fractions from 10, 5, 3 and 1 kDa hydrolysate fractions were obtained. The 5-K hydrolysate fraction had high oxidation inhibilitory ratio (42.62 %, which was about twofold higher than the original hydrolysate and as high as that of vitamin E (α-tocopherol. The fractionated hydrolysates were superior to the original hydrolysate in the antioxidative activity tested. Moreover, these separated hydrolysates showed the enhanced functional property. The amino acid composition of 5-K hydrolysate was analyzed and the results show that the high antioxidative activity of 5-K hydrolysate was derived from high content of histidine, methionine, cystine and tryptophan.

  12. Modification of the radiation resistance of Aspergillus flavus mycelial units by some chemicals

    International Nuclear Information System (INIS)

    Mohyuddin, M.; Skoropad, W.P.


    Survival curves for the mycelium of Aspergillus flavus Link var. columnaris Raper and Fennell were constructed after irradiation with gamma rays in the presence of NaCl, NaBr, NaI, KCl, KBr, KI, CaCl 2 , CaBr 2 , CaI 2 , Ca(NO 3 ) 2 , NaNO 2 , NaNO 3 , KNO 2 , iodoacetic acid, iodoacetamide and vitamin K 5 . In addition iodized salt was also tested. All the chemicals tested exhibited initial toxicity at zero dose. However, most of the chemicals demonstrated a synergism when present during irradiation. Compounds containing iodine were invariably the strongest radiosensitizers. The iodine present as an admixture in salt also retained its radiosensitizing character. Sodium bromide and calcium bromide behaved in a different way. The initial toxicity was reduced along with an increase in radiation dose resulting in more survival. (orig.) [de

  13. Quantitation of heparosan with heparin lyase III and spectrophotometry. (United States)

    Huang, Haichan; Zhao, Yingying; Lv, Shencong; Zhong, Weihong; Zhang, Fuming; Linhardt, Robert J


    Heparosan is Escherichia coli K5 capsule polysaccharide, which is the key precursor for preparing bioengineered heparin. A rapid and effective quantitative method for detecting heparosan is important in the large-scale production of heparosan. Heparin lyase III (Hep III) effectively catalyzes the heparosan depolymerization, forming unsaturated disaccharides that are measurable using a spectrophotometer at 232 nm. We report a new method for the quantitative detection of heparosan with heparin lyase III and spectrophotometry that is safer and more specific than the traditional carbazole assay. In an optimized detection system, heparosan at a minimum concentration of 0.60 g/L in fermentation broth can be detected. Copyright © 2013 Elsevier Inc. All rights reserved.

  14. Atmospheric chemistry of HFC-134a. Kinetic and mechanistic study of the CF3CFHO2 + NO2 reaction

    DEFF Research Database (Denmark)

    Møgelberg, T.E.; Nielsen, O.J.; Sehested, J.


    A pulse radiolysis system was used to study the kinetics of the reaction of CF3CFHO2 with NO2. By monitoring the rate of the decay of NO2 using its absorption at 400 nm the reaction rate constant was determined to be k = (5.0 +/- 0.5) x 10(-12) cm3 molecule-1 s-1. A long path length Fourier......-transform infrared technique was used to investigate the thermal decomposition of the product CF3CFHO2NO2. At 296 K in the presence of 700 Torr of air, decomposition of CF3CFHO2NO2 was rapid (greater than 90% decomposition within 3 min). The results are discussed in the context of atmospheric chemistry of CF3CFH2...

  15. Role of T-type calcium channels in myogenic tone of skeletal muscle resistance arteries

    DEFF Research Database (Denmark)

    VanBavel, Ed; Sorop, Oana; Andreasen, Ditte


    T-type calcium channels may be involved in the maintenance of myogenic tone. We tested their role in isolated rat cremaster arterioles obtained after CO(2) anesthesia and decapitation. Total RNA was analyzed by RT-PCR and Southern blotting for calcium channel expression. We observed expression...... of voltage-operated calcium (Ca(V)) channels Ca(V)3.1 (T-type), Ca(V)3.2 (T-type), and Ca(V)1.2 (L-type) in cremaster arterioles (n = 3 rats). Amplification products were observed only in the presence of reverse transcriptase and cDNA. Concentration-response curves of the relatively specific L-type blocker......); K(+) -5.4 +/- 0.3 (n = 4); all log(IC(50)) P maintenance of myogenic tone in rat cremaster muscle arterioles....

  16. Infrared observations of AE Aquarii (United States)

    Tanzi, E. G.; Chincarini, G.; Tarenghi, M.


    Broadband infrared observations of the cataclysmic variable AE Aquarii are reported. The observations were obtained in the J, H, K and L filters with the InSb photometer attached to the 1-m telescope of the European Southern Observatory. The infrared energy distribution observed from 0.35 to 3.5 microns for phase 0.5 suggests a spectral type of K5 V for the secondary and a distance to the system of approximately 70 pc if an absolute magnitude of 7.3 is assumed. Monitoring of the flux at 2.2 microns reveals a variability with an amplitude of approximately 0.3 magnitude over one third of the orbital period, the nature of which is under investigation.

  17. Education in Science Centers: Evaluating School Visits to an Astronomical Observatory in Brazil

    Directory of Open Access Journals (Sweden)

    Pedro Donizete Colombo Junior


    Full Text Available The present article analyzes the activity “Guided Visit of School Groups” carried out at Astronomical Observatory of the Center for Scientific and Cultural Diffusion (CDCC of University of Sao Paulo (USP with K4 and K5 pupils. The objectives of this research were to identify influences of such activity on learning of astronomical concepts and on pupils’ motivation. The results demonstrate that pupils have difficulties to understand Solar System concepts and the distances involved, on the other hand, the activity motivates the pupils to return with their parents and friends to the Observatory. At last, the success of visits to science centers aiming at the learning of basic concepts and motivation comprises at least three moments: the one that precedes the visit, the visit itself and the return to the classroom.

  18. Information transfer across intra/inter-structure of CDS and stock markets (United States)

    Lim, Kyuseong; Kim, Sehyun; Kim, Soo Yong


    We investigate the information flow between industrial sectors in credit default swap and stock markets in the United States based on transfer entropy. Both markets have been studied with respect to dynamics and relations. Our approach considers the intra-structure of each financial market as well as the inter-structure between two markets through a moving window in order to scan a period from 2005 to 2012. We examine the information transfer with different k, especially k = 3, k = 5 and k = 7. Analysis indicates that the cases with k = 3 and k = 7 show the opposite trends but similar characteristics. Change in transfer entropy for intra-structure of CDS market precedes that of stock market in view of the entire time windows. Abrupt rise and fall in inter-structural information transfer between two markets are detected at the periods related to the financial crises, which can be considered as early warnings.

  19. Noise temperature of an NbN hot-electron bolometric mixer at frequencies from 0.7 THz to 5.2 THz

    International Nuclear Information System (INIS)

    Schubert, J.; Semenov, A.; Gol'tsman, G.; Huebers, H-W.; Voronov, B.; Gershenzon, E.; Schwaab, G.


    We report on noise temperature measurements of an NbN phonon-cooled hot-electron bolometric mixer in the terahertz frequency range. The devices were 3 nm thick films with in-plane dimensions 1.7x0.2μm 2 and 0.9x0.2μm 2 integrated in a complementary logarithmic-spiral antenna. Measurements were performed at seven frequencies ranging from 0.7 THz to 5.2 THz. The measured DSB noise temperatures are 1500 K (0.7 THz), 2200 K (1.4 THz), 2600 K (1.6 THz), 2900 K (2.5 THz), 4000 K (3.1 THz), 5600 K (4.3 THz) and 8800 K (5.2 THz). (author)

  20. On-site treatment and landfilling of MSWI air pollution control residues

    DEFF Research Database (Denmark)

    Lundtorp, Kasper; Jensen, Dorthe Lærke; Sørensen, Mette Abildgaard


    the process, collected through the drainage system, contained large concentrations of salts (Cl: 14–30 g/l, Na: 4–9 g/l, K: 5–11 g/l, Ca: 2–12 g/l) but low concentrations of trace metals (e.g. Pb: 14–100 μg/l, Cd: leaching......Air pollution control (APC) residues from municipal solid waste incineration (MSWI) are difficult to landfill due to substantial leaching of trace metals. An on-site pretreatment prior to landfilling of APC-residues was investigated in terms of bench-scale experiments with a semidry APC...... of the leaching, concentrations of trace metals were reduced by up to four orders of magnitude....

  1. Oxy-combustion in CFB conditions

    Energy Technology Data Exchange (ETDEWEB)

    Tomasz Czakiert; Rafal Kobylecki; Zbigniew Bis; Waldemar Muskala; Wojciech Nowak [Czestochowa University of Technology, Czestochowa (Poland)


    Results of the investigation on oxygen-enriched combustion in a circulating fluidized bed (CFB) are presented and discussed in this paper. The information includes data on emission of CO{sub 2}, CO, NOx and SO{sub 2}. The investigations have been performed on an electrically heated laboratory-scale experimental setup with a circulating fluidized bed. Brown coal was selected as fuel. The fed gases were O{sub 2}+N{sub 2} and O{sub 2}+CO{sub 2} based mixtures with an oxygen concentration of 21%, 40% and 60%. During measurements the bed temperature was changed in the range of 973K to 1133K 5 refs., 10 figs., 2 tabs.

  2. Thermal performance measurements of a 100 percent polyester MLI [multilayer insulation] system for the Superconducting Super Collider

    International Nuclear Information System (INIS)

    Gonczy, J.D.; Boroski, W.N.; Niemann, R.C.


    The plastic materials used in the multilayer insulation (MLI) blankets of the superconducting magnets of the Superconducting Super Collider (SSC) are comprised entirely of polyesters. This paper reports on tests conducted in three separate experimental blanket arrangements. The tests explore the thermal performance of two candidate blanket joint configurations each employing a variation of a stepped-butted joint nested between sewn blanket seams. The results from the joint configurations are compared to measurements made describing the thermal performance of the basic blanket materials as tested in an ideal joint configuration. Twenty foil sensors were incorporated within each test blanket to measure interstitial layer and joint layer temperatures. Heat flux and thermal gradients are reported for high and degraded insulating vacuums, and during transient and steady state conditions. In complement with this paper is an associate paper bearing the same title head but with the title extension 'Part 1: Instrumentation and experimental preparation (300K-80K)'. 5 refs., 8 figs., 2 tabs

  3. Search for the proton decay mode p →ν ¯K+ with KamLAND (United States)

    Asakura, K.; Gando, A.; Gando, Y.; Hachiya, T.; Hayashida, S.; Ikeda, H.; Inoue, K.; Ishidoshiro, K.; Ishikawa, T.; Ishio, S.; Koga, M.; Matsuda, R.; Matsuda, S.; Mitsui, T.; Motoki, D.; Nakamura, K.; Obara, S.; Oki, Y.; Oura, T.; Shimizu, I.; Shirahata, Y.; Shirai, J.; Suzuki, A.; Tachibana, H.; Tamae, K.; Ueshima, K.; Watanabe, H.; Xu, B. D.; Yamauchi, Y.; Yoshida, H.; Kozlov, A.; Takemoto, Y.; Yoshida, S.; Fushimi, K.; Grant, C.; Piepke, A.; Banks, T. I.; Berger, B. E.; Freedman, S. J.; Fujikawa, B. K.; O'Donnell, T.; Learned, J. G.; Maricic, J.; Sakai, M.; Dazeley, S.; Svoboda, R.; Winslow, L. A.; Efremenko, Y.; Karwowski, H. J.; Markoff, D. M.; Tornow, W.; Detwiler, J. A.; Enomoto, S.; Decowski, M. P.; KamLAND Collaboration


    We present a search for the proton decay mode p →ν ¯K+ based on an exposure of 8.97 kton-years in the KamLAND experiment. The liquid scintillator detector is sensitive to successive signals from p →ν ¯K+ with unique kinematics, which allow us to achieve a detection efficiency of 44%, higher than previous searches in water Cherenkov detectors. We find no evidence of proton decays for this mode. The expected background, which is dominated by atmospheric neutrinos, is 0.9 ±0.2 events. The nonbackground-subtracted limit on the partial proton lifetime is τ /B (p →ν ¯ K+)>5.4 ×1032 years at 90% C.L.

  4. Beamtest results of ATLAS SCT Modules in 2002

    CERN Document Server

    Barr, A J; Dolezal, Z; Donega, M; D'Onofrio, M; García, J E; González, S; Horazdovsky, T; Kazi, S; Kodys, P; Moorhead, G F; Reznicek, P; Solar, M; Vos, M; Wallny, R


    Beamtests of ATLAS Semiconductor Tracker (SCT) modules carried out at the ATLAS testbeam facility at the CERN SPS H8. During 2002, three beam runs were carried out in May/June, July and August. In the August 2002 beam test period four irradiated modules, two ``K5'' end-cap and two barrel, with the final design were tested. Module propierties (efficiency, charge collection, signal/noise, pulse shape) and the dependence of them for a particle high incidence angle was studied. A comparison with previous testbeam results was also performed. Time-stamping performance of SCT modules and specially, the effect of irradiation on the time characteristics of the Front End was investigated more closely. On this note we show a summary of these studies.

  5. The TESIS Project: Are Type 2 QSO Hidden in X-Ray Emitting EROs? (United States)

    Severgnini, P.; Della Ceca, R.; Braito, V.; Saracco, P.; Longhetti, M.; Bender, R.; Drory, N.; Feulner, G.; Hopp, U.; Mannucci, F.; Maraston, C.

    X-ray selected EROs are, on average, the hardest X-ray sources in medium and deep X-ray fields. This coupled with their extremely red colors (R-K > 5) suggest that they represent one of the most promising population where looking for high-luminosity (LX > 1044 erg s-1) and X-ray obscured (NH > 1022 cm-2) type2 AGNs, the so called QSO2 (e.g., [5]; [4]; Mignoli et al. submitted to A&A). These latter are predicted in large density by the synthesis model of the Cosmic X-ray background [9] even if only few observational evidences have been found so far (e.g., [1] and references therein; Caccianiga et al. A&A accepted).

  6. Novel, broad-spectrum anticonvulsants containing a sulfamide group: advancement of N-((benzo[b]thien-3-yl)methyl)sulfamide (JNJ-26990990) into human clinical studies. (United States)

    Parker, Michael H; Smith-Swintosky, Virginia L; McComsey, David F; Huang, Yifang; Brenneman, Douglas; Klein, Brian; Malatynska, Ewa; White, H Steve; Milewski, Michael E; Herb, Mark; Finley, Michael F A; Liu, Yi; Lubin, Mary Lou; Qin, Ning; Iannucci, Robert; Leclercq, Laurent; Cuyckens, Filip; Reitz, Allen B; Maryanoff, Bruce E


    In seeking broad-spectrum anticonvulsants to treat epilepsy and other neurological disorders, we synthesized and tested a group of sulfamide derivatives (4a-k, 5), which led to the clinical development of 4a (JNJ-26990990). This compound exhibited excellent anticonvulsant activity in rodents against audiogenic, electrically induced, and chemically induced seizures, with very weak inhibition of human carbonic anhydrase-II (IC(50) = 110 microM). The pharmacological profile for 4a supports its potential in the treatment of multiple forms of epilepsy, including pharmacoresistant variants. Mechanistically, 4a inhibited voltage-gated Na(+) channels and N-type Ca(2+) channels but was not effective as a K(+) channel opener. The pharmacokinetics and metabolic properties of 4a are discussed.

  7. Photo- and radiation chemical cycloaddition of maleic acid derivatives to ethylene and acetylene under elavated pressure

    International Nuclear Information System (INIS)

    Mirbach, M.


    Based on spectroscopic and kinetic measurements the influence of high pressure on some selected photochemical cycloaddition-reactions is studied. The photo-cycloaddition-reaction of maleic acid anhydride with ethylen has been performed under high ethylen pressures ( 90%). Surprisingly the quantum yield of the cyclo aduct decreases with increasing ethylene pressure from PHI = 0.06 at p = 1 bar to PHI = 0.022 at p = 42 bar. Based on Stern-Volmer quenching experiments, the decrease in ring formation with increasing ethylene concentrations could be explained by an endoergic triplet energy transfer from maleic acid anhydride to ethylene. The type II dissociation of butyrophenone has been quenched also with ethylene. With a lifetime for the first excited butyrophenone triplett state of tau = 6.8 x 10 -8 sec, obtained from kinetic data, the velocity constant can be calculated for this reaction with the result k 5 = 3 x 10 6 M -1 sec -1 . (orig./HK) [de

  8. Factors predicting hyperkalemia in patients with cirrhosis receiving spironolactone

    International Nuclear Information System (INIS)

    Abbas, Z.; Mumtaz, K.; Salam, A.; Jafri, W.


    Objective: To evaluate the factors leading to hyperkalemia in patients with cirrhosis receiving spironolactone. Results: Patients with hyperkalemia (K>5 mmol/l) had higher blood urea nitrogen, serum creatinine and bilirubin levels (p=0.004, 0.001 and 0.044 respectively). Their serum sodium and albumin levels were lower (p=0.000 and 0.017 respectively). They had advanced cirrhosis with high Pugh score (p=0.003). These patients were on higher dose of spironolactone (p=0.001). Multivariate analysis showed that dose of spironolactone > 100 mg/day, serum creatinine >1.3 mg/dl, persistence of ascites and edema, and female gender were important predictors of development of hyperkalemia. Conclusion: Patients with cirrhosis receiving high dose of the diuretic, having edema, ascites and high serum creatinine are at the greater risk of developing hyperkalemia during spironolactone therapy. (author)

  9. Near infrared multicolor photometry of late type stars with the balloon borne astronomical telescope BAT-1

    International Nuclear Information System (INIS)

    Kodaira, Keiichi; Tanaka, Wataru; Nakada, Yoshikazu; Watanabe, Tetsuya; Onaka, Takashi


    A new star follower has been developed for observing the near infrared emission of late type stars. The sensor of the follower consists of a semicircular rotating sector and a photomultiplier. The practical accuracy of the angle of tracing was about 1 minute. A photometer was installed at the focus point of the main telescope. The infrared photometer consists of a filter turret, a chopper, an infrared detector and a synchronous amplifier. Five flights of balloons were made since September 13, 1974. The height of the flights was about 25 km. The type of observed spectra ranges from A0 to M6. The results of analysis was compared with the atmospheric model by Tsuji. The physical parameters, such as effective temperature, logarithm of surface gravity and velocity of turbulent flow, of late type stars (K5 - M6) were determined. (Kato, T.)

  10. Structure and dynamics of solvated Ba(II) in dilute aqueous solution - an ab initio QM/MM MD approach

    International Nuclear Information System (INIS)

    Hofer, Thomas S.; Rode, Bernd M.; Randolf, Bernhard R.


    Structural properties of the hydrated Ba(II) ion have been investigated by ab initio quantum mechanical/molecular mechanical (QM/MM) molecular dynamics (MD) simulations at double zeta HF quantum mechanical level. The first shell coordination number was found to be 9.3, and several other structural parameters such as angular distribution functions, radial distribution functions and tilt- and θ-angle distributions allowed the full characterization of the hydration structure of the Ba(II) ion in dilute aqueous solution. Velocity autocorrelation functions were used to calculate librational and vibrational motions, ion-ligand motions as well as reorientation times. Different dynamical parameters such as water reorientation, mean ligand residence time, the number of ligand exchange processes and rate constants were also analyzed and the ligand exchange rate constant for the first shell was determined as k = 5.3 x 10 10 s -1

  11. Observation of new charmless decays of bottom hadrons. (United States)

    Aaltonen, T; Adelman, J; Akimoto, T; Alvarez González, B; Amerio, S; Amidei, D; Anastassov, A; Annovi, A; Antos, J; Apollinari, G; Apresyan, A; Arisawa, T; Artikov, A; Ashmanskas, W; Attal, A; Aurisano, A; Azfar, F; Azzurri, P; Badgett, W; Barbaro-Galtieri, A; Barnes, V E; Barnett, B A; Bartsch, V; Bauer, G; Beauchemin, P-H; Bedeschi, F; Beecher, D; Behari, S; Bellettini, G; Bellinger, J; Benjamin, D; Beretvas, A; Beringer, J; Bhatti, A; Binkley, M; Bisello, D; Bizjak, I; Blair, R E; Blocker, C; Blumenfeld, B; Bocci, A; Bodek, A; Boisvert, V; Bolla, G; Bortoletto, D; Boudreau, J; Boveia, A; Brau, B; Bridgeman, A; Brigliadori, L; Bromberg, C; Brubaker, E; Budagov, J; Budd, H S; Budd, S; Burke, S; Burkett, K; Busetto, G; Bussey, P; Buzatu, A; Byrum, K L; Cabrera, S; Calancha, C; Campanelli, M; Campbell, M; Canelli, F; Canepa, A; Carls, B; Carlsmith, D; Carosi, R; Carrillo, S; Carron, S; Casal, B; Casarsa, M; Castro, A; Catastini, P; Cauz, D; Cavaliere, V; Cavalli-Sforza, M; Cerri, A; Cerrito, L; Chang, S H; Chen, Y C; Chertok, M; Chiarelli, G; Chlachidze, G; Chlebana, F; Cho, K; Chokheli, D; Chou, J P; Choudalakis, G; Chuang, S H; Chung, K; Chung, W H; Chung, Y S; Chwalek, T; Ciobanu, C I; Ciocci, M A; Clark, A; Clark, D; Compostella, G; Convery, M E; Conway, J; Cordelli, M; Cortiana, G; Cox, C A; Cox, D J; Crescioli, F; Cuenca Almenar, C; Cuevas, J; Culbertson, R; Cully, J C; Dagenhart, D; Datta, M; Davies, T; de Barbaro, P; De Cecco, S; Deisher, A; De Lorenzo, G; Dell'Orso, M; Deluca, C; Demortier, L; Deng, J; Deninno, M; Derwent, P F; di Giovanni, G P; Dionisi, C; Di Ruzza, B; Dittmann, J R; D'Onofrio, M; Donati, S; Dong, P; Donini, J; Dorigo, T; Dube, S; Efron, J; Elagin, A; Erbacher, R; Errede, D; Errede, S; Eusebi, R; Fang, H C; Farrington, S; Fedorko, W T; Feild, R G; Feindt, M; Fernandez, J P; Ferrazza, C; Field, R; Flanagan, G; Forrest, R; Frank, M J; Franklin, M; Freeman, J C; Furic, I; Gallinaro, M; Galyardt, J; Garberson, F; Garcia, J E; Garfinkel, A F; Genser, K; Gerberich, H; Gerdes, D; Gessler, A; Giagu, S; Giakoumopoulou, V; Giannetti, P; Gibson, K; Gimmell, J L; Ginsburg, C M; Giokaris, N; Giordani, M; Giromini, P; Giunta, M; Giurgiu, G; Glagolev, V; Glenzinski, D; Gold, M; Goldschmidt, N; Golossanov, A; Gomez, G; Gomez-Ceballos, G; Goncharov, M; González, O; Gorelov, I; Goshaw, A T; Goulianos, K; Gresele, A; Grinstein, S; Grosso-Pilcher, C; Grundler, U; Guimaraes da Costa, J; Gunay-Unalan, Z; Haber, C; Hahn, K; Hahn, S R; Halkiadakis, E; Han, B-Y; Han, J Y; Happacher, F; Hara, K; Hare, D; Hare, M; Harper, S; Harr, R F; Harris, R M; Hartz, M; Hatakeyama, K; Hays, C; Heck, M; Heijboer, A; Heinrich, J; Henderson, C; Herndon, M; Heuser, J; Hewamanage, S; Hidas, D; Hill, C S; Hirschbuehl, D; Hocker, A; Hou, S; Houlden, M; Hsu, S-C; Huffman, B T; Hughes, R E; Husemann, U; Hussein, M; Huston, J; Incandela, J; Introzzi, G; Iori, M; Ivanov, A; James, E; Jang, D; Jayatilaka, B; Jeon, E J; Jha, M K; Jindariani, S; Johnson, W; Jones, M; Joo, K K; Jun, S Y; Jung, J E; Junk, T R; Kamon, T; Kar, D; Karchin, P E; Kato, Y; Kephart, R; Keung, J; Khotilovich, V; Kilminster, B; Kim, D H; Kim, H S; Kim, H W; Kim, J E; Kim, M J; Kim, S B; Kim, S H; Kim, Y K; Kimura, N; Kirsch, L; Klimenko, S; Knuteson, B; Ko, B R; Kondo, K; Kong, D J; Konigsberg, J; Korytov, A; Kotwal, A V; Kreps, M; Kroll, J; Krop, D; Krumnack, N; Kruse, M; Krutelyov, V; Kubo, T; Kuhr, T; Kulkarni, N P; Kurata, M; Kwang, S; Laasanen, A T; Lami, S; Lammel, S; Lancaster, M; Lander, R L; Lannon, K; Lath, A; Latino, G; Lazzizzera, I; LeCompte, T; Lee, E; Lee, H S; Lee, S W; Leone, S; Lewis, J D; Lin, C-S; Linacre, J; Lindgren, M; Lipeles, E; Lister, A; Litvintsev, D O; Liu, C; Liu, T; Lockyer, N S; Loginov, A; Loreti, M; Lovas, L; Lucchesi, D; Luci, C; Lueck, J; Lujan, P; Lukens, P; Lungu, G; Lyons, L; Lys, J; Lysak, R; Macqueen, D; Madrak, R; Maeshima, K; Makhoul, K; Maki, T; Maksimovic, P; Malde, S; Malik, S; Manca, G; Manousakis-Katsikakis, A; Margaroli, F; Marino, C; Marino, C P; Martin, A; Martin, V; Martínez, M; Martínez-Ballarín, R; Maruyama, T; Mastrandrea, P; Masubuchi, T; Mathis, M; Mattson, M E; Mazzanti, P; McFarland, K S; McIntyre, P; McNulty, R; Mehta, A; Mehtala, P; Menzione, A; Merkel, P; Mesropian, C; Miao, T; Miladinovic, N; Miller, R; Mills, C; Milnik, M; Mitra, A; Mitselmakher, G; Miyake, H; Moggi, N; Moon, C S; Moore, R; Morello, M J; Morlock, J; Movilla Fernandez, P; Mülmenstädt, J; Mukherjee, A; Muller, Th; Mumford, R; Murat, P; Mussini, M; Nachtman, J; Nagai, Y; Nagano, A; Naganoma, J; Nakamura, K; Nakano, I; Napier, A; Necula, V; Nett, J; Neu, C; Neubauer, M S; Neubauer, S; Nielsen, J; Nodulman, L; Norman, M; Norniella, O; Nurse, E; Oakes, L; Oh, S H; Oh, Y D; Oksuzian, I; Okusawa, T; Orava, R; Osterberg, K; Griso, S Pagan; Palencia, E; Papadimitriou, V; Papaikonomou, A; Paramonov, A A; Parks, B; Pashapour, S; Patrick, J; Pauletta, G; Paulini, M; Paus, C; Peiffer, T; Pellett, D E; Penzo, A; Phillips, T J; Piacentino, G; Pianori, E; Pinera, L; Pitts, K; Plager, C; Pondrom, L; Poukhov, O; Pounder, N; Prakoshyn, F; Pronko, A; Proudfoot, J; Ptohos, F; Pueschel, E; Punzi, G; Pursley, J; Rademacker, J; Rahaman, A; Ramakrishnan, V; Ranjan, N; Redondo, I; Renton, P; Renz, M; Rescigno, M; Richter, S; Rimondi, F; Ristori, L; Robson, A; Rodrigo, T; Rodriguez, T; Rogers, E; Rolli, S; Roser, R; Rossi, M; Rossin, R; Roy, P; Ruiz, A; Russ, J; Rusu, V; Saarikko, H; Safonov, A; Sakumoto, W K; Saltó, O; Santi, L; Sarkar, S; Sartori, L; Sato, K; Savoy-Navarro, A; Schlabach, P; Schmidt, A; Schmidt, E E; Schmidt, M A; Schmidt, M P; Schmitt, M; Schwarz, T; Scodellaro, L; Scribano, A; Scuri, F; Sedov, A; Seidel, S; Seiya, Y; Semenov, A; Sexton-Kennedy, L; Sforza, F; Sfyrla, A; Shalhout, S Z; Shears, T; Shepard, P F; Shimojima, M; Shiraishi, S; Shochet, M; Shon, Y; Shreyber, I; Sidoti, A; Sinervo, P; Sisakyan, A; Slaughter, A J; Slaunwhite, J; Sliwa, K; Smith, J R; Snider, F D; Snihur, R; Soha, A; Somalwar, S; Sorin, V; Spalding, J; Spreitzer, T; Squillacioti, P; Stanitzki, M; St Denis, R; Stelzer, B; Stelzer-Chilton, O; Stentz, D; Strologas, J; Strycker, G L; Stuart, D; Suh, J S; Sukhanov, A; Suslov, I; Suzuki, T; Taffard, A; Takashima, R; Takeuchi, Y; Tanaka, R; Tecchio, M; Teng, P K; Terashi, K; Tesarek, R; Thom, J; Thompson, A S; Thompson, G A; Thomson, E; Tipton, P; Ttito-Guzmán, P; Tkaczyk, S; Toback, D; Tokar, S; Tollefson, K; Tomura, T; Tonelli, D; Torre, S; Torretta, D; Totaro, P; Tourneur, S; Trovato, M; Tsai, S-Y; Tu, Y; Turini, N; Ukegawa, F; Vallecorsa, S; van Remortel, N; Varganov, A; Vataga, E; Vázquez, F; Velev, G; Vellidis, C; Vidal, M; Vidal, R; Vila, I; Vilar, R; Vine, T; Vogel, M; Volobouev, I; Volpi, G; Wagner, P; Wagner, R G; Wagner, R L; Wagner, W; Wagner-Kuhr, J; Wakisaka, T; Wallny, R; Wang, S M; Warburton, A; Waters, D; Weinberger, M; Weinelt, J; Wester, W C; Whitehouse, B; Whiteson, D; Wicklund, A B; Wicklund, E; Wilbur, S; Williams, G; Williams, H H; Wilson, P; Winer, B L; Wittich, P; Wolbers, S; Wolfe, C; Wright, T; Wu, X; Würthwein, F; Xie, S; Yagil, A; Yamamoto, K; Yamaoka, J; Yang, U K; Yang, Y C; Yao, W M; Yeh, G P; Yoh, J; Yorita, K; Yoshida, T; Yu, G B; Yu, I; Yu, S S; Yun, J C; Zanello, L; Zanetti, A; Zhang, X; Zheng, Y; Zucchelli, S


    We search for new charmless decays of neutral b hadrons to pairs of charged hadrons, using 1 fb(-1) of data collected by the CDF II detector at the Fermilab Tevatron. We report the first observation of the Bs0-->K-pi+ decay and measure B(Bs0-->K-pi+)=(5.0+/-0.7(stat)+/-0.8(syst))x10(-6). We also report the first observation of charmless b-baryon decays, and measure B(Lambdab0-->ppi-)=(3.5+/-0.6(stat)+/-0.9(syst))x10(-6) and B(Lambdab0-->pK-)=(5.6+/-0.8(stat)+/-1.5(syst))x10(-6). No evidence is found for other modes, and we set the limit B(Bs0-->pi+pi;-)<1.2x10(-6) at 90% C.L.

  12. Forward and inverse cascades in decaying two-dimensional electron magnetohydrodynamic turbulence

    International Nuclear Information System (INIS)

    Wareing, C. J.; Hollerbach, R.


    Electron magnetohydrodynamic (EMHD) turbulence in two dimensions is studied via high-resolution numerical simulations with a normal diffusivity. The resulting energy spectra asymptotically approach a k -5/2 law with increasing R B , the ratio of the nonlinear to linear time scales in the governing equation. No evidence is found of a dissipative cutoff, consistent with nonlocal spectral energy transfer. Dissipative cutoffs found in previous studies are explained as artificial effects of hyperdiffusivity. Relatively stationary structures are found to develop in time, rather than the variability found in ordinary or MHD turbulence. Further, EMHD turbulence displays scale-dependent anisotropy with reduced energy transfer in the direction parallel to the uniform background field, consistent with previous studies. Finally, the governing equation is found to yield an inverse cascade, at least partially transferring magnetic energy from small to large scales.

  13. JARE Syowa Station 11-m Antenna, Antarctica (United States)

    Aoyama, Yuichi; Doi, Koichiro; Shibuya, Kazuo


    In 2012, the 52nd and the 53rd Japanese Antarctic Research Expeditions (hereinafter, referred to as JARE-52 and JARE-53, respectively) participated in five OHIG sessions - OHIG76, 78, 79, 80, and 81. These data were recorded on hard disks through the K5 terminal. Only the hard disks for the OHIG76 session have been brought back from Syowa Station to Japan, in April 2012, by the icebreaker, Shirase, while those of the other four sessions are scheduled to arrive in April 2013. The data obtained from the OHIG73, 74, 75, and 76 sessions by JARE-52 and JARE-53 have been transferred to the Bonn Correlator via the servers of National Institute of Information and Communications Technology (NICT). At Syowa Station, JARE-53 and JARE-54 will participate in six OHIG sessions in 2013.

  14. Investigation of hydrogen adsorption centers on Y2O3 by IR-spectroscopy method in diffusive-scattered light

    International Nuclear Information System (INIS)

    Zubkov, S.A.; Borovkov, V.Yu.


    Adsorption of hydrogen and carbon oxide at the yttrium oxide at 80 K (5x30 3 PaH 2 ) and 300 K (6.5x10 2 PaCO) respectively are studied by the method of IR spectroscopy. It is shown, that at the surface of yttrium oxide trained in vacuum at 970 K, at least four types of centres of hydrogen adsorption, able to polarize H-H bond in a molecule, exist. Acid-base couple is the highest polarized centre, in the content of which there is a coordination-unsaturated highly-charged yttrium cation. Low-temperature dissociation of hydrogen on Y 2 O 3 surface occurs on the centres which polarized H-H bond in molecule comparatively slow

  15. A Synthesis of Research on Informational Text Reading Interventions for Elementary Students With Learning Disabilities. (United States)

    Ciullo, Stephen; Lo, Yu-Ling Sabrina; Wanzek, Jeanne; Reed, Deborah K


    This research synthesis was conducted to understand the effectiveness of interventions designed to improve learning from informational text for students with learning disabilities in elementary school (K-5). The authors identified 18 studies through a comprehensive search. The interventions were evaluated to determine treatment effects and to understand implementation and methodological variables that influenced outcomes. Moderate to large effect sizes on researcher-developed measures for cognitive strategy interventions were reported. Interventions that utilized graphic organizers as study guides to support social studies learning were also associated with improved outcomes. The findings are considered within the context of limited implementation of standardized measures. The authors extend findings from previous research by reporting a paucity of interventions to enhance higher-level cognitive and comprehension skills. The majority of reviewed studies targeted fact acquisition and main idea identification, and overall encouraging findings were noted for these skills. Implications for future research are discussed. © Hammill Institute on Disabilities 2014.

  16. The great escape: viral strategies to counter BST-2/tetherin.

    Directory of Open Access Journals (Sweden)

    Janet L Douglas


    Full Text Available The interferon-induced BST-2 protein has the unique ability to restrict the egress of HIV-1, Kaposi's sarcoma-associated herpesvirus (KSHV, Ebola virus, and other enveloped viruses. The observation that virions remain attached to the surface of BST-2-expressing cells led to the renaming of BST-2 as "tetherin". However, viral proteins such as HIV-1 Vpu, simian immunodeficiency virus Nef, and KSHV K5 counteract BST-2, thereby allowing mature virions to readily escape from infected cells. Since the anti-viral function of BST-2 was discovered, there has been an explosion of research into several aspects of this intriguing interplay between host and virus. This review focuses on recent work addressing the molecular mechanisms involved in BST-2 restriction of viral egress and the species-specific countermeasures employed by various viruses.

  17. Dicty_cDB: Contig-U06758-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 129 4e-29 EU016618_33( EU016618 |pid:none) Uncultured marine microorganism H... 129 4e-29 (Q12WQ7) RecName: Full=Putati...... 54 0.005 1 ( EE147587 ) SiJWA07BAA2 Lausanne fire ant library Solenopsis ... 44 0.008 2 ( BW805727 ) Bra...nidia eriantha youn... 44 4.4 1 ( EU016618 ) Uncultured marine O-sialoglycoprotein endopeptidas... 119 3e-26 EU016589_20( EU016589 |pid:none) Uncultured Group I marine cre...das... 58 1e-07 (Q3K5S1) RecName: Full=Probable O-sialoglycoprotein endopeptidas... 58 1e-07 AB290257_1( AB290257 |pid:none) Uncultur

  18. Linearized method: A new approach for kinetic analysis of central dopamine D2 receptor specific binding

    International Nuclear Information System (INIS)

    Watabe, Hiroshi; Hatazawa, Jun; Ishiwata, Kiichi; Ido, Tatsuo; Itoh, Masatoshi; Iwata, Ren; Nakamura, Takashi; Takahashi, Toshihiro; Hatano, Kentaro


    The authors proposed a new method (Linearized method) to analyze neuroleptic ligand-receptor specific binding in a human brain using positron emission tomography (PET). They derived the linear equation to solve four rate constants, k 3 , k 4 , k 5 , k 6 from PET data. This method does not demand radioactivity curve in plasma as an input function to brain, and can do fast calculations in order to determine rate constants. They also tested Nonlinearized method including nonlinear equations which is conventional analysis using plasma radioactivity corrected for ligand metabolites as an input function. The authors applied these methods to evaluate dopamine D 2 receptor specific binding of [ 11 C] YM-09151-2. The value of B max /K d = k 3 k 4 obtained by Linearized method was 5.72 ± 3.1 which was consistent with the value of 5.78 ± 3.4 obtained by Nonlinearized method

  19. Transcription of human 7S K DNA in vitro and in vivo is exclusively controlled by an upstream promoter

    Energy Technology Data Exchange (ETDEWEB)

    Kleinert, H.; Benecke, B.J.


    The authors have analyzed the transcription of a recently isolated human 7S K RNA gene in vitro and in vivo. In contrast to hitherto characterized class III genes (genes transcribed by RNA polymerase III), the coding sequence of this gene is not required for faithful and efficient transcription by RNA polymerase III. In fact, a procaryotic vector DNA sequence was efficiently transcribed by RNA polymerase III under the control of the 7S K RNA gene upstream sequence in vitro and in vivo. S/sub 1/-nuclease protection analyses confirmed that the 7S K 5'flanking sequence was sufficient for accurate transcription initiation. These data demonstrate that 7S K DNA represents a novel class III gene, the promoter elements of which are located outside the coding sequence.

  20. Export of nutrients from golf courses on the Precambrian Shield

    International Nuclear Information System (INIS)

    Winter, Jennifer G.; Dillon, Peter J.


    Annual export rates, or fluxes, of total nitrogen (TN), nitrate, total phosphorus (TP) and potassium from four streams on two golf courses on the Precambrian Shield were compared with those from forested reference locations. Overall, the mean annual fluxes of K, TN, NO 3 and TP from golf courses were greater than from forested areas by 10, 2, 6 and 2 times, respectively. The overall mean export coefficients (kg/ha/yr) were 16 for K, 5.2 for TN, 2.1 for NO 3 and 0.14 for TP. For TN and TP, these are similar to those reported from cropland in Canada by Chambers and Dale (1997. Contribution of industrial, municipal, agricultural and groundwater sources to nutrient export, Athabasca, Wapiti and Smoky Rivers, 1980 to 1993. Northern River Basins Study Project Report No. 110. Northern River Basins Study, Edmonton, Alberta). -- Golf courses increase nutrient loads in receiving streams

  1. Design, construction, and performance of superconducting magnet support posts for the Large Hadron Collider

    International Nuclear Information System (INIS)

    Blin, M.; Danielsson, H.; Evans, B.; Mathieu, M.


    Different support posts for the Large Hadron Collider (LHC) prototype superconducting magnets have been designed and manufactured. They have been evaluated both mechanically and thermally. The posts are made of a tubular section in composite materials, i.e. glass- or carbon-fibre and epoxy resin, with glued metallic heat intercepts and connections. Mechanical tests have been carried out with both radial and axial loads, before and after cooldown to working temperature. The design considerations and future developments concerning dimensions and other materials are also discussed in this paper. Thermal performance has been evaluated at 1.8 K, 5 K and 80 K in a precision heat leak measuring bench. The measurements have been carried out using calibrated thermal conductances (open-quotes heatmetersclose quotes) and boil-off methods. The measured performances of the posts have been compared with analytical predictions

  2. High frequency characterization of Galfenol minor flux density loops

    Directory of Open Access Journals (Sweden)

    Ling Weng


    Full Text Available This paper presents the first measurement of ring-shaped Galfenol’s high frequency-dependent minor flux density loops. The frequencies of applied AC magnetic field are 1k, 5k, 10k, 50k, 100k, 200k, 300k, 500 kHz. The measurements show that the cycle area between the flux density and magnetic field curves increase with increasing frequency. High frequency-dependent characterization, including coercivity, specific power loss, residual induction, and maximum relative permeability are discussed. Minor loops for different max induction are also measured and discussed at the same frequency 100 kHz. Minor loops with the same max induction 0.05 T for different frequencies 50, 100, 200, 300, 400 kHz are measured and specific power loss are discussed.


    International Nuclear Information System (INIS)

    Gettel, S.; Wolszczan, A.; Niedzielski, A.; Nowak, G.; Adamów, M.; Zieliński, P.; Maciejewski, G.


    We present the discovery of planet-mass companions to two giant stars by the ongoing Penn State-Toruń Planet Search conducted with the 9.2 m Hobby-Eberly Telescope. The less massive of these stars, K5-giant BD+20 274, has a 4.2 M J minimum mass planet orbiting the star at a 578 day period and a more distant, likely stellar-mass companion. The best currently available model of the planet orbiting the K0-giant HD 219415 points to a ∼> Jupiter-mass companion in a 5.7 year, eccentric orbit around the star, making it the longest period planet yet detected by our survey. This planet has an amplitude of ∼18 m s –1 , comparable to the median radial velocity 'jitter', typical of giant stars.

  4. Practical experience with own monitoring of clay sealings on dumps for brown coal-fired power station residues. Erfahrungen mit der Eigenueberwachung an Tonabdichtungen der Deponien fuer Braunkohlenkraftwerksrueckstaende

    Energy Technology Data Exchange (ETDEWEB)

    Winter, K


    In order to dispose of power station residues Rheinbraun erects dumps which are encased by a clay sealing. For the Fortuna dump 10 hectares sealing area, with a permeability that is lower than the required limit value of k = 5x10{sup -10} m/s, have so far been constructed. The quality of the sealing is monitored and ensured by graded checking. The practical experience gained so far with own monitoring (selection of material, supervision of construction site and own checking) on the finished clay sealings is discussed in the present paper. The system of testing the sealings has proved very satisfactory. The results of the tests carried out in the course of own monitoring show that processing work in the laboratory can be reduced by systematic selection of material and intensive expert supervision of the construction site. (orig.).

  5. Applicability of Taylor's hypothesis in thermally driven turbulence (United States)

    Kumar, Abhishek; Verma, Mahendra K.


    In this paper, we show that, in the presence of large-scale circulation (LSC), Taylor's hypothesis can be invoked to deduce the energy spectrum in thermal convection using real-space probes, a popular experimental tool. We perform numerical simulation of turbulent convection in a cube and observe that the velocity field follows Kolmogorov's spectrum (k-5/3). We also record the velocity time series using real-space probes near the lateral walls. The corresponding frequency spectrum exhibits Kolmogorov's spectrum (f-5/3), thus validating Taylor's hypothesis with the steady LSC playing the role of a mean velocity field. The aforementioned findings based on real-space probes provide valuable inputs for experimental measurements used for studying the spectrum of convective turbulence.

  6. Do stressed mothers have heavier children?: A meta-analysis on the relationship between maternal stress and child body mass index (United States)

    Tate, Eleanor B.; Wood, Wendy; Liao, Yue; Dunton, Genevieve F.


    Child obesity continues to be a prevalent public health issue. This meta-analysis synthesized 17 studies investigating the association between levels of psychological stress experienced by mothers and the body mass index of their children. The overall standardized mean difference effect size was positive and significantly different from zero in cross-sectional d = 0.20 [k = 14, 95% Confidence Interval (CI): 0.06, 0.34] and longitudinal studies d = 0.18 (k = 5, 95% CI: 0.00, 0.351), and had significant heterogeneity in both [cross-sectional, Q(13) = 193.00, p children also would have experienced the stressor, Q(6) = 4.68, p stress management component in childhood obesity prevention programs. PMID:25879393

  7. Assessing the Resilience of Global Sea Routes (United States)


    Salmeron Thesis Advisor Gerald G. Brown Second Reader Robert F. Dell Chair, Department of Operations Research iv THIS PAGE...Japan 730.10 639.10 1.14 Korea South 464.30 422.40 1.10 Malaysia 197.00 152.60 1.29 Netherlands 486.70 429.50 1.13 Norway 132.70 74.30 1.79 Pakistan...9.1%, Singapore 8.7%, South Korea 8%, India 6.3%, Malaysia 5.9% Italy Germany 13.2%, France 11.7%, Spain 5.9%, U.S. 5.8%, U.K. 5.4%, Switzerland 4.6

  8. CSI: Dognapping workshop : an outreach experiment designed to produce students that are hooked on science.

    Energy Technology Data Exchange (ETDEWEB)

    Boyle, Timothy J.; Gorman, Anna K.; Pratt, Harry D., III; Hernandez-Sanchez, Bernadette A.; Lambert, Timothy N.; Ottley, Leigh Anna M.; Baros, Christina Marie


    The CSI: Dognapping Workshop is a culmination of the more than 65 Sandian staff and intern volunteers dedication to exciting and encouraging the next generation of scientific leaders. This 2 hour workshop used a 'theatrical play' and 'hands on' activities that was fun, exciting and challenging for 3rd-5th graders while meeting science curriculum standards. In addition, new pedagogical methods were developed in order to introduce nanotechnology to the public. Survey analysis indicated that the workshop had an overall improvement and positive impact on helping the students to understand concepts from materials science and chemistry as well as increased our interaction with the K-5 community. Anecdotal analyses showed that this simple exercise will have far reaching impact with the results necessary to maintain the United States as the scientific leader in the world. This experience led to the initiation of over 100 Official Junior Scientists.

  9. Fast repair of oxidizing OH adducts of DNA by hydroxycinnamic acid derivatives. A pulse radiolytic study

    International Nuclear Information System (INIS)

    Yue Jiang; Lin Weizhen; Yao Side; Lin Nianyun; Zhu Dayuan


    Using pulse radiolytic techniques, it has been demonstrated that the interactions of oxidizing OH adducts of DNA (ssDNA and dsDNA), polyA and polyG with hydroxycinnamic acid derivatives proceed via an electron transfer process (k=5-30x10 8 dm 3 mol -1 s -1 ). In addition, the rates for fast repair of OH adducts of dAMP, polyA and DNA (ssDNA and dsDNA) are slower than the corresponding rates for the rest OH adducts of DNA constituents. The slower rates for repair of oxidizing OH adducts of dAMP may be the rate determining step during the interaction of hydroxycinnamic acid derivatives with OH adducts of DNA containing the varieties of OH adducts of DNA constituents

  10. The soft supersymmetry breaking in D=5 supergravity compactified on S_1/Z_2 orbifolds

    CERN Document Server

    Diamandis, G A; Kouroumalou, P; Lahanas, A B


    We study the origin of the supersymmetry breaking induced by the mediation of gravity and the radion multiplet from the hidden to the visible brane in the context of the N=2, D=5 supergravity compactified on S_1/Z_2 orbifolds. The soft supersymmetry breaking terms for scalar masses, trilinear scalar couplings and gaugino masses are calculated to leading order in the five dimensional Newton's constant k_5^2 and the gravitino mass m_{3/2}. These are finite and non-vanishing, with the scalar soft masses be non-tachyonic, and are all expressed in terms of the gravitino mass and the length scale R of the fifth dimension. The soft supersymmetry breaking parameters are thus correlated and the phenomenological implications are discussed.

  11. The Prediction of Metal Slopping in LD Converter on Base an Acoustic Signal

    Directory of Open Access Journals (Sweden)

    Kostúr, K.


    Full Text Available The negative influences of slopping in a BOF are pollution to the environment. They give lower yield and cause equipment damage. The prediction of these phenomena is based on information processing from the measuring microphone. The change of frequency in certain range is done by a signal for the prediction of slopping. In this paper two methods for prediction of slopping are described. The first method is based on measuring and processing of sound emitted from the vessel during the blow. The second method utilizes Fourier’s transformation for processing of acoustic signal from sonic meter. The success rate of prediction has been evaluated by help of five criterions. It is possible to forecast the slopping on selected frequency (band. It is the essence of the second method, because this method has high success (criterion K1. Note, that criterion K5 defines acknowledgment of duration slopping. This criterion has the highest value.

  12. YSOVAR: Six Pre-main-sequence Eclipsing Binaries in the Orion Nebula Cluster (United States)


    ISOY J053446.01−044922.1i V1448 Ori 17.43c 15.61c 13.88 13.26 12.95 12.73 12.66 12.91 . . . K5c 0.5424125 ISOY J053605.95−050041.2i 2MASS J05360595...identified 12 EB candidates. Four of them, 2MASS 05352184−0546085 (Stassun et al. 2006), V1174 Ori (Stassun et al. 2004), Par1802 (Cargile et al. 2008...Zero points for the photometry were established by reference to data for non-variable stars in our FOV from the Two Micron All Sky Survey ( 2MASS

  13. Rotorcraft Flight Simulation Computer Program C81 with Datamap Interface, Volume 2. Programmer’s Manual (United States)


    overview of the computer program capabilities and the principal mathematical models incorporated in the program are given in Volume I of the documentation...blank card must still be placed in the appropriate place in the Model Data Set. For example, the mathematical model of a UH-lH would not need a wing...SIN %4114 LV OADs CC £6 $ 2. 27 4L 4+ 4 l’l <m L & % CC O 04i18K5 twsSTVEL 13L4.S2 ASK 2? b 41.815 . 8 ’ILbLS a CL 7 8 £0 1 £0 £0 12 £6 1 M90 A/ MUfI H

  14. Time-resolved fluorescence study of exciplex formation in diastereomeric naproxen-pyrrolidine dyads. (United States)

    Khramtsova, Ekaterina A; Plyusnin, Viktor F; Magin, Ilya M; Kruppa, Alexander I; Polyakov, Nikolay E; Leshina, Tatyana V; Nuin, Edurne; Marin, M Luisa; Miranda, Miguel A


    The influence of chirality on the elementary processes triggered by excitation of the (S,S)- and (R,S)- diastereoisomers of naproxen-pyrrolidine (NPX-Pyr) dyads has been studied by time-resolved fluorescence in acetonitrile-benzene mixtures. In these systems, the quenching of the (1)NPX*-Pyr singlet excited state occurs through electron transfer and exciplex formation. Fluorescence lifetimes and quantum yields revealed a significant difference (around 20%) between the (S,S)- and (R,S)- diastereomers. In addition, the quantum yields of exciplexes differed by a factor of 2 regardless of solvent polarity. This allows us to suggest a similar influence of the chiral centers on the local charge transfer resulting in exciplex and full charge separation that leads to ion-biradicals. A simplified scheme is proposed to estimate a set of rate constant values (k1-k5) for the elementary stages in each solvent system.

  15. Implementing a Manualized, Classroom Transition Intervention for Students With ASD in Underresourced Schools. (United States)

    Iadarola, Suzannah; Shih, Wendy; Dean, Michelle; Blanch, Erica; Harwood, Robin; Hetherington, Susan; Mandell, David; Kasari, Connie; Smith, Tristram


    Children with autism spectrum disorder (ASD) in public education settings experience difficulties with transitions during classroom routines, which can result in challenging behavior. Single-subject research supports techniques for transitions, but school-based approaches often require resources and training unavailable in low-resource districts, limiting implementation. We developed and evaluated the Schedules, Tools, and Activities for Transitions (STAT) program, a short-term, manualized intervention of behavioral supports to support daily routine transitions for students with ASD (K-5) in underresourced districts. We utilized a multisite, cluster-randomized, group comparison design (immediate treatment versus waitlist) with matched pairs ( n = 150 students, 57 educators). Data indicated (a) no group differences for academic engagement or classroom independence, and (b) an advantage for STAT in reducing challenging behavior and increasing teacher fidelity. Results show preliminary support for an intervention that is feasible and perceived as sustainable in real-world settings.

  16. Genetic diversity of serotype A foot-and-mouth disease viruses in Kenya from 1964 to 2013; implications for control strategies in eastern Africa

    DEFF Research Database (Denmark)

    Wekesa, Sabenzia N.; Sangula, Abraham K.; Belsham, Graham


    Serotype A is the most genetically and antigenically diverse of the foot-and-mouth disease virus (FMDV) serotypes. Records of its occurrence in Kenya date back to 1952 and the antigenic diversity of the outbreak viruses in this region is reflected by the current use of two different vaccine strains...... (K5/1980 and K35/1980) and previous use of two other strains (K18/66 and K179/71). This study aimed at enhancing the understanding of the patterns of genetic variation of serotype A FMDV in Kenya. The complete VP1 coding region sequences of 38 field isolates, identified as serotype A FMDV, collected...... between 1964 and 2013 were determined. Coalescent-based methods were used to infer times of divergence of the virus strains and the evolutionary rates alongside 27 other serotype A FMDV sequences from Genbank and the World Reference Laboratory (WRL). This study represents the first comprehensive genetic...

  17. Complexation of Bi{sup 3+}, Ac{sup 3+}, Y{sup 3+}, Lu{sup 3+}, La{sup 3+} and Eu{sup 3+} with benzo-diaza-crown ether with carboxylic pendant arms

    Energy Technology Data Exchange (ETDEWEB)

    Egorova, Bayirta V. [Lomonosov Moscow State Univ., Moscow (Russian Federation). Chemistry Dept.; Oshchepkov, Maxim S.; Fedorova, Olga A. [Mendeleev University of Chemistry and Technology of Russia, Moscow (Russian Federation); Russian Academy of Sciences, Moscow (Russian Federation). A.N. Nesmeyanov Institute of Organoelement Compounds; Fedorov, Yury V. [Lomonosov Moscow State Univ., Moscow (Russian Federation). Chemistry Dept.; Russian Academy of Sciences, Moscow (Russian Federation). A.N. Nesmeyanov Institute of Organoelement Compounds; Budylin, Gleb S.; Shirshin, Evgeny A. [Lomonosov Moscow State Univ., Moscow (Russian Federation). Dept. of Physics; Kalmykov, Stepan N. [Lomonosov Moscow State Univ., Moscow (Russian Federation). Chemistry Dept.; National Research Center ' Kurchatov Institute' , Moscow (Russian Federation)


    Polyaminopolycarboxylates are attractive ligands for binding cationic radionuclides for synthesis of radiopharmaceuticals with target delivery to tumor cells. Nowadays beta emitting Y-90 and Lu-177 are used as therapeutic agents, while Ac-225 and Bi-213 are considered as perspective for alpha therapy. In the present study new data on complexation of Y{sup 3+}, Lu{sup 3+}, Ac{sup 3+} and Bi{sup 3+} with 2,2'-(15-formyl-2,3,5,6,8,9,11,12-octahydrobenzo [b][1,4,10,7,13]trioxadiazacyclopentadecine-4,10- diyl)diacetic acid are presented. For ligand and complexes characterization potentiometric titration, solvent extraction, chromatography and solubility techniques were applied. The highest values of stability constants within the range of log K = 5.8 - 7.5 were found for Ac{sup 3+} and REE. Fast complex formation is established which is beneficial for practical applications in radiopharmaceutical synthesis.

  18. Strong pinning in very fast grown reactive co-evaporated GdBa2Cu3O7 coated conductors

    Directory of Open Access Journals (Sweden)

    J. L. MacManus-Driscoll


    Full Text Available We report on compositional tuning to create excellent field-performance of Jc in “self-doped,” GdBa2Cu3O7−y (GdBCO coated conductors grown by ultrafast reactive co-evaporation. In order to give excess liquid and Gd2O3, the overall compositions were all Ba-poor and Cu-rich compared to GdBCO. The precise composition was found to be critical to the current carrying performance. The most copper-rich composition had an optimum self-field Jc of 3.2 MA cm−2. A more Gd-rich composition had the best in-field performance because of the formation of low coherence, splayed Gd2O3 nanoparticles, giving Jc (77 K, 1 T of over 1 MA cm−2 and Jc (77 K, 5 T of over 0.1 MA cm−2.

  19. Design of tangential viewing phase contrast imaging for turbulence measurements in JT-60SA

    Energy Technology Data Exchange (ETDEWEB)

    Tanaka, K., E-mail: [National Institute for Fusion Science, Toki, Gifu 509-5292 (Japan); Department of Advanced Energy Engineering, Kyushu University, Kasuga, Fukuoka 816-8580 (Japan); Coda, S. [EPFL–SPC, Lausanne (Switzerland); Yoshida, M.; Sasao, H.; Kawano, Y.; Imazawa, R.; Kubo, H.; Kamada, Y. [National Institutes for Quantum and Radiological Science and Technology, Naka, Ibaraki 311-0193 (Japan)


    A tangential viewing phase contrast imaging system is being designed for the JT-60SA tokamak to investigate microturbulence. In order to obtain localized information on the turbulence, a spatial-filtering technique is applied, based on magnetic shearing. The tangential viewing geometry enhances the radial localization. The probing laser beam is injected tangentially and traverses the entire plasma region including both low and high field sides. The spatial resolution for an Internal Transport Barrier discharge is estimated at 30%–70% of the minor radius at k = 5 cm{sup −1}, which is the typical expected wave number of ion scale turbulence such as ion temperature gradient/trapped electron mode.

  20. Spectroscopy of late type giant stars (United States)

    Spaenhauer, A.; Thevenin, F.


    An attempt to calibrate broadband RGU colors of late type giant stars in terms of the physical parameters of the objects is reported. The parameters comprise the effective temperature, surface gravity and global metal abundance with respect to the sun. A selection of 21 giant star candidates in the Basel fields Plaut 1, Centaurus III and near HD 95540 were examined to obtain a two color plot. Attention is focused on the G-R color range 1.5-2.15 mag, i.e., spectral types K0-K5. A relationship between R and the metallicity is quantified and shown to have a correlation coefficient of 0.93. No correlation is found between metallicity and gravity or R and the effective temperature.

  1. Towards the physical vacuum of cosmic inflation

    Directory of Open Access Journals (Sweden)

    Hongliang Jiang


    Full Text Available There have been long debates about the initial conditions of inflationary perturbations. In this work we explicitly show the decay of excited states during inflation via interactions from the renormalization group point of view. For this purpose, we note that the folded shape non-Gaussianity can be interpreted as the decay of the non-Bunch–Davies initial condition. The one loop diagrams with non-Bunch–Davies propagators are calculated to uncover the decay of such excited states. We find that the decay of amplitude is contributed by the folded shape of loop momentum and is irrelevant to the UV part. The conformal decay rate is related to the strength of non-Gaussianity via Γ∼(fNLλ2Pζk5O(τ4,τ04. The observed smallness of non-Gaussianity keeps the window open for probing inflationary initial conditions and trans-Planckian physics.

  2. Creating a Bio-Inspired Solution to Prevent Erosion (United States)

    Reher, R.; Martinez, A.; Cola, J.; Frost, D.


    Through the study of geophysical sciences, lessons can be developed which allow for the introduction of bio-inspired design and art concepts to K-5 elementary students. Students are placed into an engineering mindset in which they must apply the concepts of bio-geotechnics to observe how we can use nature to prevent and abate erosion. Problems are staged for students using realistic engineering scenarios such as erosion prevention through biomimicry and the study of anchorage characteristics of root structures in regard to stability of soil. Specifically, a lesson is introduced where students research, learn, and present information about bio-inspired designs to understand these concepts. They lean how plant roots differ in size and shape to stabilize soil. In addition, students perform a series of hands-on experiments which demonstrate how bio-cements and roots can slow erosion.

  3. Cabernet Sauvignon grapevine grafted onto rootstocks during the autumn-winter season in southeastern Brazilian

    Directory of Open Access Journals (Sweden)

    Claudia Rita de Souza


    Full Text Available The change of grape (Vitis vinifera harvest from summer to winter through double pruning management has improved the fine wine quality in southern Brazil. High altitude, late cultivar and grafting combination all need to be investigated to optimize this new viticulture management. For this purpose, this study was carried out during the 2011 and 2012 growing seasons in a high altitude region of the state of Minas Gerais, Brazil, using eight grafting combinations for five year old Cabernet Sauvignon vines. The stem water potential, photosynthetic rate and stomatal conductance were not affected by rootstock type. The rootstocks IAC 766 and 101-14 induced, respectively, the highest and lowest vegetative vigor in Cabernet Sauvignon, as shown by leaf area and pruning weight. In the 2011 growing season, the leaf chlorophyll contents were increased in IAC 766, whereas vines grafted onto 101-14 accumulated more leaf starch, probably due to reduced vegetative and reproductive growth. In general, rootstocks K5BB, 1045P, SO4 and IAC 766 had the highest yield as compared to 1103P and 101-14. Berries from the grapevine with the highest yield did not differ in pH, total soluble solids and acidity. The rootstocks did not influence the anthocyanins and total phenols in both growing seasons. Quality parameters were better in the 2011 than in the 2012 growing season due to better climatic conditions, mainly less rainfall. The best performance of Cabernet Sauvignon was achieved when grafted onto K5BB, 1045P, SO4 and IAC 766 rootstocks.

  4. Improving virtual screening predictive accuracy of Human kallikrein 5 inhibitors using machine learning models. (United States)

    Fang, Xingang; Bagui, Sikha; Bagui, Subhash


    The readily available high throughput screening (HTS) data from the PubChem database provides an opportunity for mining of small molecules in a variety of biological systems using machine learning techniques. From the thousands of available molecular descriptors developed to encode useful chemical information representing the characteristics of molecules, descriptor selection is an essential step in building an optimal quantitative structural-activity relationship (QSAR) model. For the development of a systematic descriptor selection strategy, we need the understanding of the relationship between: (i) the descriptor selection; (ii) the choice of the machine learning model; and (iii) the characteristics of the target bio-molecule. In this work, we employed the Signature descriptor to generate a dataset on the Human kallikrein 5 (hK 5) inhibition confirmatory assay data and compared multiple classification models including logistic regression, support vector machine, random forest and k-nearest neighbor. Under optimal conditions, the logistic regression model provided extremely high overall accuracy (98%) and precision (90%), with good sensitivity (65%) in the cross validation test. In testing the primary HTS screening data with more than 200K molecular structures, the logistic regression model exhibited the capability of eliminating more than 99.9% of the inactive structures. As part of our exploration of the descriptor-model-target relationship, the excellent predictive performance of the combination of the Signature descriptor and the logistic regression model on the assay data of the Human kallikrein 5 (hK 5) target suggested a feasible descriptor/model selection strategy on similar targets. Copyright © 2017 Elsevier Ltd. All rights reserved.

  5. Estandarizacion y evaluacion de un enzimoinmunoensayo para diagnostico y seroepidemiologia de triquinosis humana

    Directory of Open Access Journals (Sweden)

    Emílio Coltorti


    Full Text Available Se estandarizó un enzimoinmunoensayo (EIE en fase sólida en el que se empleó un antígeno soluble total de T. spiralis y una dilución única de suero, y se evaluó la capacidad del sistema para detectar anticuerpos totales antitriquina con fines diagnósticos y seroepidemiológicos. Las curvas dosis respuesta mostraron que trabajando con una dilución de suero de 1:100 se obtenía una buena diferenciación entre sueros de indivíduos no infectados y pacientes de triquinosis con baja concentración de anticuerpos circulantes y la respuesta fue lineal entre valores de 0.15 y 0.64 unidades de densidad óptica (DO. El estúdio de 720 sueros de población general no infectada revelo una X k = 0.07 y una S = 0.03 unidades de DO. El 98,61% de esta población presentó valores de DO X k + 3S y X k + 4S y < X k + 5S. Se empleó un valor de DO = X k + 5S como nivel diagnóstico para estudiar 4 brotes de triquinosis humana, 80 sueros de pacientes de triquinosis con diferentes títulos en inmunofluorescencia indirecta (IFI y 20 sueros de pacientes de hidatidosis. El EIE propuesto permitió diferenciar en forma confiable y eficiente población infectada de no infectada y tuvo una buena correlación con la IFI. Todos los sueros de pacientes hidatídicos presentaron valores de DO < X k + 4S.

  6. Mixing of radiolytic hydrogen generated within a containment compartment following a LOCA

    International Nuclear Information System (INIS)

    Willcutt, G.J.E. Jr.; Gido, R.G.


    The objective of this work was to determine hydrogen concentration variations with position and time in a closed containment compartment with radiolytic hydrogen generation in the water on the compartment floor following a Loss-of-Coolant-Accident (LOCA). One application is to determine the potential difference between the compartment maximum hydrogen concentration and a hydrogen detector reading, due to the detector location. Three possible mechanisms for hydrogen transport in the compartment were investigated: (1) molecular diffusion, (2) possible bubble formation and motion, and (3) natural convection flows. A base case cubic compartment with 6.55-m (21.5-ft) height was analyzed. Parameter studies were used to determine the sensitivity of results to compartment size, hydrogen generation rates, diffusion coefficients, and the temperature difference between the floor and the ceiling and walls of the compartment. Diffusion modeling indicates that if no other mixing mechanism is present for the base case, the maximum hydrogen volume percent (vol percent) concentration difference between the compartment floor and ceiling will be 4.8 percent. It will be 24.5 days before the maximum concentration difference is less than 0.5 percent. Bubbles do not appear to be a potential source of hydrogen pocketing in a containment compartment. Compartment natural convection circulation rates for a 2.8 K (5 0 F) temperature difference between the floor and the ceiling and walls are estimated to be at least the equivalent of 1 compartment volume per hour and probably in the range of 4 to 9 compartment volumes per hour. Related natural convection studies indicate there will be turbulent mixing in the compartment for a 2.8 K (5 0 F) temperature difference between the floor and the ceiling and walls

  7. Response of tomato to different concentrations of nutrients Respuesta del tomate chonto cultivar Unapal Maravilla,

    Directory of Open Access Journals (Sweden)

    Mejía de Tafur Maria Sara


    Full Text Available In the greenhouse of the Universidad Nacional de Colombia at Palmira branch an experiment to determine the nutritional requirements of tomato UNAPAL Maravilla under hidroponic system, using reactive degree salts and distilled water was carried out. The data demonstrated that the suitable stage of development to determine the nutricional requirements is 47 days after planting, since the plants are in preflowering, shortly before beginning the linear phase of growth. The nutritionale requirement of tomato is: N, 13 - 20 mM; P, 1 - 1,5 mM; K, 5 - 8 mM; Ca, 4 - 6 mM; and Mg, 2 - 4 mM.En las casas de malla de la Universidad Nacional de Colombia Sede Palmira se realizó un experimento con el objetivo de determinar los requerimientos nutricionales del tomate UNAPAL Maravilla para lo cual se empleó el sistema de hidroponía con sales grado reactivo y agua destilada y se escogió un diseño completamente al azar con 16 tratamientos y cinco repeticiones, se realizaron evaluaciones a los 27, 34, 42, 54 y 92 dds. La etapa de desarrollo adecuada para determinar los requerimientos nutricionales se presentó a los 47 dds en prefloración, poco antes de comenzar la fase lineal de crecimiento. Los requerimientos de N estuvieron entre 13 - 20 mM; los de P, 1 - 1.5 mM; los de K, 5 - 8 mM; los de Ca, 4 - 6 mM; y los de Mg, 2 - 4 mM.

  8. Keratin 5/14‑mediated cell differentiation and transformation are regulated by TAp63 and Notch‑1 in oral squamous cell carcinoma‑derived cells. (United States)

    Srivastava, Saumya S; Alam, Hunain; Patil, Sonam J; Shrinivasan, Rashmi; Raikundalia, Sweta; Chaudhari, Pratik Rajeev; Vaidya, Milind M


    Keratins 5/14 (K5/14) are intermediate filament proteins expressed in the basal layer of stratified epithelial cells and are known targets of p63. Previous research in our laboratory showed that upon K5/14 downregulation in oral squamous cell carcinoma (OSCC)‑derived cells, there was an increase in intracellular Notch‑1 levels and differentiation markers such as involucrin, keratin 1 and a decrease in tumorigenic potential in vivo. However, the molecules involved in the K14 regulated cell differentiation and transformation are not known to date. In order to understand the possible role of TAp63, we downregulated TAp63 in a K14‑knockdown background. We observed that there was a decrease in the expression of Notch‑1. Expression levels of differentiation markers such as involucrin, K1, loricrin and filaggrin were also decreased. Furthermore, TAp63 downregulation led to an increase in invasion, migration and in vivo tumorigenic potential of these cells. We observed a decrease in β‑catenin signaling in K14‑downregulated cells. Notably, when TAp63 was downregulated in K14‑knockdown cells, there was increase in non‑phospho β‑catenin levels. Hence, this study indicates that TAp63 plays an important role in K14‑downregulated cells possibly by regulating the Notch‑1 expression. K14 regulates the expression of TAp63 which in turn regulates expression of Notch‑1. The present study is a step forward in our quest to understand the functional significance of molecules that regulate the process of differentiation and tumorigenesis in stratified epithelial cells.

  9. Dynamic behaviour of raft and pile foundations tests and computational models. Pt. 1

    International Nuclear Information System (INIS)

    Betbeder, J.; Garnier, J.C.; Gauvain, J.; Jeandidier, C.


    Pile foundations are commonly used for many types of buildings where the bearing capacity of soil is poor. For nuclear power plants buildings, however, there seems to be a fairly general reluctancy to accept design on piles, as it is considered difficult to demonstrate the safety of these foundations with respect to earthquakes, due to the relative lack of validation of the currently available aseismic design methods. Being conscious that pile foundations might be worth considering for future nuclear sites in France and that the reliability of design methods should be backed by experimental data, ELECTRICITE DE FRANCE decided in 1978 to undertake a series of tests, aimed at assessing the validity of computational models for seismic behaviour of pile foundations and trying to define better models if necessary. These tests on reduced scale structure, including various types of raft and pile foundations and different kinds of dynamic excitation (harmonic, earthquake simulation, impulsive release of a static force) have been made at the NICE airport site. The present paper deals with the general description of the tests and the first part of interpretation work, limited to in-structure harmonic excitation and earthquake simulation tests analyzed by simple spring -dashpot analytical models. The two following papers (K5-6 and K5-7) are devoted to specialized topics in relation with the interpretation of tests, i-e ground motions analysis for earthquake simulation and research work on a new computational model. Although preliminary conclusions can be drawn from the results obtained so far, further work will be necessary to reach a conclusive assessment on this difficult subject. (orig.)

  10. Phenotypic and molecular characterization of Klebsiella spp. isolates causing community-acquired infections

    Directory of Open Access Journals (Sweden)

    U. Garza-Ramos


    Full Text Available Klebsiella spp. isolates from community-acquired infections were characterized. A total of 39 Klebsiella spp. isolates were obtained from outpatients at four rural hospitals in Mexico (2013–2014. The biochemical tests identified all as being K. pneumoniae. The molecular multiplex-PCR test identified 36 (92.4% K. pneumoniae isolates and one (2.5% K. variicola isolate, and phylogenetic analysis of the rpoB gene identified two isolates (5.1% belonging to K. quasipneumoniae subsp. quasipneumoniae and K. quasivariicola. The last one was confirmed by phylogenetic analysis of six-loci concatenated genes. Mostly the isolates were multidrug resistant; however, a minority were extended-spectrum β-lactamase producing (10.2%. The extended-spectrum β-lactamase CTX-M-15 gene was identified in these isolates. Analysis of biofilm production and the hypermucoviscosity phenotype showed a total of 35 (92.3% and seven (17.9% of the isolates were positive for these phenotypes respectively. The K2 (4/39, 10.2%, K5 (2/39, 5.1% and K54 (1/39, 2.5% serotypes were identified in seven (17.9% of the isolates, and only 28.5% (2/7 hypermucoviscous isolates were positive for the K2 and K5 serotypes. In general, the sequence type (ST analysis and phylogenetic analysis of seven multilocus sequence typing loci were heterogeneous; however, ST29 was the most prevalent ST in the analysed isolates, accounting for 19% (4/21 of the total isolates. Two of the four ST29 isolates had the hypermucoviscosity phenotype. The virulence factors for fimbriae were the most prevalent, followed by siderophores. Community-acquired infections are caused by various species from Klebsiella genus, with different profiles of antibiotic resistance and heterogeneous virulence factors. Keywords: Antimicrobial susceptibility, Bacterial resistance, Cephalosporin resistance, Community infection, ESBL, Hypermucoviscosity

  11. Energy Spectra of Vortex Distributions in Two-Dimensional Quantum Turbulence

    Directory of Open Access Journals (Sweden)

    Ashton S. Bradley


    Full Text Available We theoretically explore key concepts of two-dimensional turbulence in a homogeneous compressible superfluid described by a dissipative two-dimensional Gross-Pitaeveskii equation. Such a fluid supports quantized vortices that have a size characterized by the healing length ξ. We show that, for the divergence-free portion of the superfluid velocity field, the kinetic-energy spectrum over wave number k may be decomposed into an ultraviolet regime (k≫ξ^{-1} having a universal k^{-3} scaling arising from the vortex core structure, and an infrared regime (k≪ξ^{-1} with a spectrum that arises purely from the configuration of the vortices. The Novikov power-law distribution of intervortex distances with exponent -1/3 for vortices of the same sign of circulation leads to an infrared kinetic-energy spectrum with a Kolmogorov k^{-5/3} power law, which is consistent with the existence of an inertial range. The presence of these k^{-3} and k^{-5/3} power laws, together with the constraint of continuity at the smallest configurational scale k≈ξ^{-1}, allows us to derive a new analytical expression for the Kolmogorov constant that we test against a numerical simulation of a forced homogeneous, compressible, two-dimensional superfluid. The numerical simulation corroborates our analysis of the spectral features of the kinetic-energy distribution, once we introduce the concept of a clustered fraction consisting of the fraction of vortices that have the same sign of circulation as their nearest neighboring vortices. Our analysis presents a new approach to understanding two-dimensional quantum turbulence and interpreting similarities and differences with classical two-dimensional turbulence, and suggests new methods to characterize vortex turbulence in two-dimensional quantum fluids via vortex position and circulation measurements.

  12. Histone Acetylation Modifications Affect Tissue-Dependent Expression of Poplar Homologs of C4 Photosynthetic Enzyme Genes

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    Yuan Li


    Full Text Available Histone modifications play important roles in regulating the expression of C4 photosynthetic genes. Given that all enzymes required for the C4 photosynthesis pathway are present in C3 plants, it has been hypothesized that this expression regulatory mechanism has been conserved. However, the relationship between histone modification and the expression of homologs of C4 photosynthetic enzyme genes has not been well determined in C3 plants. In the present study, we cloned nine hybrid poplar (Populus simonii × Populus nigra homologs of maize (Zea mays C4 photosynthetic enzyme genes, carbonic anhydrase (CA, pyruvate orthophosphate dikinase (PPDK, phosphoenolpyruvate carboxykinase (PCK, and phosphoenolpyruvate carboxylase (PEPC, and investigated the correlation between the expression levels of these genes and the levels of promoter histone acetylation modifications in four vegetative tissues. We found that poplar homologs of C4 homologous genes had tissue-dependent expression patterns that were mostly well-correlated with the level of histone acetylation modification (H3K9ac and H4K5ac determined by chromatin immunoprecipitation assays. Treatment with the histone deacetylase inhibitor trichostatin A further confirmed the role of histone acetylation in the regulation of the nine target genes. Collectively, these results suggest that both H3K9ac and H4K5ac positively regulate the tissue-dependent expression pattern of the PsnCAs, PsnPPDKs, PsnPCKs, and PsnPEPCs genes and that this regulatory mechanism seems to be conserved among the C3 and C4 species. Our findings provide new insight that will aid efforts to modify the expression pattern of these homologs of C4 genes to engineer C4 plants from C3 plants.

  13. PSYM-WIDE: A Survey for Large-separation Planetary-mass Companions to Late Spectral Type Members of Young Moving Groups (United States)

    Naud, Marie-Eve; Artigau, Étienne; Doyon, René; Malo, Lison; Gagné, Jonathan; Lafrenière, David; Wolf, Christian; Magnier, Eugene A.


    We present the results of a direct imaging survey for very large separation (>100 au), low-mass companions around 95 nearby young K5-L5 stars and brown dwarfs. They are high-likelihood candidates or confirmed members of the young (≲150 Myr) β Pictoris and AB Doradus moving groups (ABDMG) and the TW Hya, Tucana-Horologium, Columba, Carina, and Argus associations. Images in I\\prime and z\\prime filters were obtained with the Gemini Multi-Object Spectrograph (GMOS) on Gemini South to search for companions down to an apparent magnitude of z\\prime ˜ 22-24 at separations ≳20″ from the targets and in the remainder of the wide 5.‧5 × 5.‧5 GMOS field of view. This allowed us to probe the most distant region where planetary-mass companions could be gravitationally bound to the targets. This region was left largely unstudied by past high-contrast imaging surveys, which probed much closer-in separations. This survey led to the discovery of a planetary-mass (9-13 {M}{Jup}) companion at 2000 au from the M3V star GU Psc, a highly probable member of ABDMG. No other substellar companions were identified. These results allowed us to constrain the frequency of distant planetary-mass companions (5-13 {M}{Jup}) to {0.84}-0.66+6.73% (95% confidence) at semimajor axes between 500 and 5000 au around young K5-L5 stars and brown dwarfs. This is consistent with other studies suggesting that gravitationally bound planetary-mass companions at wide separations from low-mass stars are relatively rare.

  14. Effects of Na and K co-doping on growth and scintillation properties of Eu:SrI_2 crystals

    International Nuclear Information System (INIS)

    Ito, Tomoki; Yokota, Yuui; Kurosawa, Shunsuke; Kral, Robert; Pejchal, Jan; Ohashi, Yuji; Kamada, Kei; Nikl, Martin; Yoshikawa, Akira


    We grew Na and K co-doped Eu:SrI_2 [Na,Eu:SrI_2 and K,Eu:SrI_2] crystals by a modified micro-pulling-down method to reveal the co-doping effects on the crystal growth and scintillation properties. The non-codoped, Na0.5%, Na1.0%, K0.5% and K1.0%,Eu:SrI_2 crystals indicated high transparency while the milky parts were generated in the Na5.0% and K5.0%,Eu:SrI_2 crystals. The light yields of Na,Eu:SrI_2 and K,Eu:SrI_2 crystals under γ-ray irradiation were decreased by the Na and K co-doping. On the other hand, there was a small change within 940–1020 ns in the decay times by the Na and K co-doping. In the light yield proportionality under γ-ray irradiation, the non-proportionality in the low energy region was improved by Na and K co-doping. - Highlights: • Na or K co-doped Eu:SrI_2crystals were grown by the modified μ-PD method. • The milky parts were generated in the Na5.0% and K5.0%,Eu:SrI_2crystals. • The light yield of Eu:SrI_2was decreased by the Na or K co-doping. • The decay times of Eu:SrI_2were almost constant by the Na or K co-doping. • The non-proportionalitywas improved in the low energy region by the K co-doping.

  15. Evaluation of oil-leakage of multi-layered resin-hose clamped with metal nipple and sleeve (United States)

    Matsuoka, Kenta; Okubo, Kazuya; Fujii, Toru; Nakamura, Chihiro; Fujishita, Yushi; Kusu, Fuko; Matsushita, Masato; Yoshihara, Ryota


    The purpose of this study is to investigate the path of occurred oil-leakage of multi-layered resin-hose as one of multifunctional materials around the caulked joint with a metal nipple and sleeve when excessive cyclic internal pressure was applied onto the hose. Equivalent cyclic axial tensile force was substitutively applied to the hose, where same degree of normal stress was produced in longitudinal direction. Excessive 3 and 5 times of the standard load was applied to the hose. Cyclic loading was paused at every 1000 and 10000 cycles and then designed internal pressure was applied to the hose by a hand-operated pump with water in order to check whether the leakage was occurred around the joint and surface of the hose for safety evaluation. Cyclic fatigue life was defined as the number of loading cycles in which the leakage and the initial damage which was the passage of the ultrasonic wave was observed on the cyclic test. Test results showed the fatigue life at which leakage of water was observed was increased 20 times in case of K=3 compared to that in case of K=5. The cycles of initial damage detected by the ultrasonic wave were passed was increased 3.3 times in case of K=3 compared to that in case of K=5. The fluorescent agent penetrated from the core layer of resin hose to the reinforcement layer in which a half cross section along longitudinal direction in failed specimens was observed after the leak test. The original specimens had the gap between the resin-hose and the nipple and then the gap extended and connected during fatigue cyclic. In this study, it was observed that oil was leaked through narrow gap between the nipple and core layer of resin hose.

  16. The Polycistronic miR166k-166h Positively Regulates Rice Immunity via Post-transcriptional Control of EIN2

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    Raquel Salvador-Guirao


    Full Text Available MicroRNAs (miRNAs are small RNAs acting as regulators of gene expression at the post-transcriptional level. In plants, most miRNAs are generated from independent transcriptional units, and only a few polycistronic miRNAs have been described. miR166 is a conserved miRNA in plants targeting the HD-ZIP III transcription factor genes. Here, we show that a polycistronic miRNA comprising two miR166 family members, miR166k and miR166h, functions as a positive regulator of rice immunity. Rice plants with activated MIR166k-166h expression showed enhanced resistance to infection by the fungal pathogens Magnaporthe oryzae and Fusarium fujikuroi, the causal agents of the rice blast and bakanae disease, respectively. Disease resistance in rice plants with activated MIR166k-166h expression was associated with a stronger expression of defense responses during pathogen infection. Stronger induction of MIR166k-166h expression occurred in resistant but not susceptible rice cultivars. Notably, the ethylene-insensitive 2 (EIN2 gene was identified as a novel target gene for miR166k. The regulatory role of the miR166h-166k polycistron on the newly identified target gene results from the activity of the miR166k-5p specie generated from the miR166k-166h precursor. Collectively, our findings support a role for miR166k-5p in rice immunity by controlling EIN2 expression. Because rice blast is one of the most destructive diseases of cultivated rice worldwide, unraveling miR166k-166h-mediated mechanisms underlying blast resistance could ultimately help in designing appropriate strategies for rice protection.

  17. Elementary Teachers' Perceptions of Teaching Science to Improve Student Content Knowledge (United States)

    Stephenson, Robert L.

    The majority of Grade 5 students demonstrate limited science knowledge on state assessments. This trend has been documented since 2010 with no evidence of improvement. Because state accountability formulas include proficiency scores and carry sanctions against districts that fail to meet proficiency thresholds, improved student performance in science is an important issue to school districts. The purpose of this study was to explore elementary teachers' perceptions about their students' science knowledge, the strategies used to teach science, the barriers affecting science teaching, and the self-efficacy beliefs teachers maintain for teaching science. This study, guided by Vygotsky's social constructivist theory and Bandura's concept of self-efficacy, was a bounded instrumental case study in which 15 participants, required to be teaching K-5 elementary science in the county, were interviewed. An analytic technique was used to review the qualitative interview data through open coding, clustering, and analytical coding resulting in identified categorical themes that addressed the research questions. Key findings reflect students' limited content knowledge in earth and physical science. Teachers identified barriers including limited science instructional time, poor curricular resources, few professional learning opportunities, concern about new state standards, and a lack of teaching confidence. To improve student content knowledge, teachers identified the need for professional development. The project is a professional development series provided by a regional education service agency for K-5 teachers to experience science and engineering 3-dimensional learning. Area students will demonstrate deeper science content knowledge and benefit from improved science instructional practice and learning opportunities to become science problem solvers and innovative contributors to society.

  18. Production of bioinspired and rationally designed polymer hydrogels for controlled delivery of therapeutic proteins (United States)

    Kim, Sung Hye

    patterns of functional groups. However, heterogeneity in the composition and in the polydispersity of heparin has been problematic in controlled delivery system and thus motivated the development of homogeneous heparin mimics. Peptides of appropriate sequence and chemical function have therefore recently emerged as potential replacements for heparin in select applications. Studied was the assessment of the binding affinities of multiple sulfated peptides (SPs) for a set of heparin-binding peptides (HBPs) and for VEGF; these binding partners have application in the selective immobilization of proteins and in hydrogel formation through non-covalent interactions. Sulfated peptides were produced via solid-phase methods, and their affinity for the HBPs and VEGF was assessed via affinity liquid chromatography (ALC), surface plasmon resonance (SPR), and in select cases, isothermal titration calorimetry (ITC). The shortest peptide, SPa, showed the highest affinity binding of HBPs and VEGF165 in both ALC and SPR measurements, with slight exceptions. Of the investigated HBPs, a peptide based on the heparin-binding domain of human platelet factor 4 showed greatest binding affinities toward all of the SPs, consistent with its stronger binding to heparin. The affinity between SPa and PF4ZIP was indicated via SPR ( KD = 5.27 muM) and confirmed via ITC (KD = 8.09 muM). The binding by SPa of both VEGF and HBPs suggests its use as a binding partner to multiple species, and the use of these interactions in assembly of materials. Given that the peptide sequences can be varied to control binding affinity and selectivity, opportunities are also suggested for the production of a wider array of matrices with selective binding and release properties useful for biomaterials applications. Hydrogel consisting of SPa was formed via a covalent Michael Addition reaction between maleimide- and thiol-terminated multi-arm PEGs and Cys-SPa. The mechanical property of hydrogel was tunable from ca. 186 to

  19. Comprehensive enzymatic analysis of the cellulolytic system in digestive fluid of the Sea Hare Aplysia kurodai. Efficient glucose release from sea lettuce by synergistic action of 45 kDa endoglucanase and 210 kDa ß-glucosidase.

    Directory of Open Access Journals (Sweden)

    Akihiko Tsuji

    Full Text Available Although many endo-ß-1,4-glucanases have been isolated in invertebrates, their cellulolytic systems are not fully understood. In particular, gastropod feeding on seaweed is considered an excellent model system for production of bioethanol and renewable bioenergy from third-generation feedstocks (microalgae and seaweeds. In this study, enzymes involved in the conversion of cellulose and other polysaccharides to glucose in digestive fluids of the sea hare (Aplysia kurodai were screened and characterized to determine how the sea hare obtains glucose from sea lettuce (Ulva pertusa. Four endo-ß-1,4-glucanases (21K, 45K, 65K, and 95K cellulase and 2 ß-glucosidases (110K and 210K were purified to a homogeneous state, and the synergistic action of these enzymes during cellulose digestion was analyzed. All cellulases exhibited cellulase and lichenase activities and showed distinct cleavage specificities against cellooligosaccharides and filter paper. Filter paper was digested to cellobiose, cellotriose, and cellotetraose by 21K cellulase, whereas 45K and 65K enzymes hydrolyzed the filter paper to cellobiose and glucose. 210K ß-glucosidase showed unique substrate specificity against synthetic and natural substrates, and 4-methylumbelliferyl (4MU-ß-glucoside, 4MU-ß-galactoside, cello-oligosaccharides, laminarin, and lichenan were suitable substrates. Furthermore, 210K ß-glucosidase possesses lactase activity. Although ß-glucosidase and cellulase are necessary for efficient hydrolysis of carboxymethylcellulose to glucose, laminarin is hydrolyzed to glucose only by 210K ß-glucosidase. Kinetic analysis of the inhibition of 210K ß-glucosidase by D-glucono-1,5-lactone suggested the presence of 2 active sites similar to those of mammalian lactase-phlorizin hydrolase. Saccharification of sea lettuce was considerably stimulated by the synergistic action of 45K cellulase and 210K ß-glucosidase. Our results indicate that 45K cellulase and 210K

  20. Продуктивность костреца безостого в зависимости от внекорневой подкормки

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    Full Text Available This paper presents the results of three-year research regarding the influence of the dates and dose of foliar fertilization using the organic microfertilizer Ekogreyn on the seed yield of smooth brome grass (variety Mars. According to the research results, it was found out that the organic microfertilizer Ekogreyn has a positive effect on the growth and development of plants of smooth brome grass, as well as on the levelof their productivity. The best indicators of seed yield were recorded by the experimental variant, as it undergone two foliar fertilizations using the organic microfertilizer Ekogreyn in the phase of stem elongation (1,3 l/ha-1 and earing (1,3 l/ha combined with the application of the basic fertilizer N60 P45 K45. The level of seed productivity in this variant was of 300 kg/ha, which is by 134 kg/ha or 80.4% more than in the variant without foliar fertilization (control variant. Реферат. Изложены результаты трехлетних исследований влияния срока и нормы внесения внекорневой подкормки органическим микроудобрением Екогрейн на урожайность семян костреца безостого сорта Марс. По результатам исследований выявлено, что органическое микроудобрение Екогрейн положительно влияло на рост и развитие растений костреца безостого, а также на уровень урожайности данной культуры. Наилучшие показатели урожайности семян костреца были отмечены на варианте опыта, где проводили две внекорневые подкормки в фазе выхода в трубку (1,3 л/га и колошения (1,3 л/га на фоне

  1. Effect of Chelator Conjugation Level and Injection Dose on Tumor and Organ Uptake of 111In Labeled MORAb-009, an Anti-mesothelin Antibody (United States)

    Shin, I. S.; Lee, S.-M.; Kim, H. S.; Yao, Z.; Regino, C.; Sato, N.; Cheng, K. T.; Hassan, R.; Campo, M. F.; Albone, E. F.; Choyke, P. L.; Pastan, I.; Paik, C. H.


    Introduction Radiolabeling of a monoclonal antibody (mAb) with a metallic radionuclide requires the conjugation of a bifunctional chelator to the mAb. The conjugation, however, can alter the physical and immunological properties of the mAb, consequently affecting its tumor targeting pharmacokinetics. In this study, we investigated the effect of the amount of 2-(p-isothiocyanatobenzyl)-cyclohexyl-diethylenetriamine-pentaacetic acid (CHX-A″) conjugated to MORAb-009, a mAb directed against mesothelin and the effect of MORAb dose on the biodistribution of 111In labeled MORAb-009. Methods We used nude mice bearing A431/K5 tumor as a mesothelin-positive tumor model and A431 tumor as a mesothelin-negative control. To find the optimal level of CHX-A″ conjugation, CHX-A″-MORAb-009 conjugates with 2.4, 3.5, and 5.5 CHX-A″ molecules were investigated. To investigate the effect of injected MORAb-009 dose on neutralizing the shed-mesothelin in the circulation, the biodistribution studies were performed after the i.v. co-injection of the 111In labeled MORAb-009 (2.4 CHX-A″/MORAb-009) with three different doses, 0.2, 2, and 30 μg of MORAb-009. Results The tumor uptake in A431/K5 tumor was 4 times higher than that in A431 tumor, indicating that the tumor uptake in A431/K5 was mesothelin-mediated. The conjugate with 5.5 CHX-A″ showed a lower isoelectric point (pI) and lower immunoreactivity (IR) than the 2.4 CHX-A″ conjugate. These differences were reflected in biodistribution of the 111In label. The 111In labeled MORAb-009 conjugated with 2.4 CHX-A″ produced higher tumor uptake, and lower liver and spleen uptakes than the 5.5 CHX-A″ conjugate. The biodistribution studies also revealed that the tumor uptake was significantly affected by the injected MORAb-009 dose and tumor size. The 30 μg dose produced higher tumor uptake than the 0.2 and 2 μg doses whereas the 30 μg dose produced lower liver and spleen uptakes than the 0.2 μg dose. Conclusion This study

  2. 18F-FLT uptake kinetics in head and neck squamous cell carcinoma: A PET imaging study

    International Nuclear Information System (INIS)

    Liu, Dan; Fenwick, John D.; Chalkidou, Anastasia; Landau, David B.; Marsden, Paul K.


    Purpose: To analyze the kinetics of 3 ′ -deoxy-3 ′ -[F-18]-fluorothymidine (18F-FLT) uptake by head and neck squamous cell carcinomas and involved nodes imaged using positron emission tomography (PET). Methods: Two- and three-tissue compartment models were fitted to 12 tumor time-activity-curves (TACs) obtained for 6 structures (tumors or involved nodes) imaged in ten dynamic PET studies of 1 h duration, carried out for five patients. The ability of the models to describe the data was assessed using a runs test, the Akaike information criterion (AIC) and leave-one-out cross-validation. To generate parametric maps the models were also fitted to TACs of individual voxels. Correlations between maps of different parameters were characterized using Pearson'sr coefficient; in particular the phosphorylation rate-constants k 3-2tiss and k 5 of the two- and three-tissue models were studied alongside the flux parameters K FLT-2tiss and K FLT of these models, and standardized uptake values (SUV). A methodology based on expectation-maximization clustering and the Bayesian information criterion (“EM-BIC clustering”) was used to distil the information from noisy parametric images. Results: Fits of two-tissue models 2C3K and 2C4K and three-tissue models 3C5K and 3C6K comprising three, four, five, and six rate-constants, respectively, pass the runs test for 4, 8, 10, and 11 of 12 tumor TACs. The three-tissue models have lower AIC and cross-validation scores for nine of the 12 tumors. Overall the 3C6K model has the lowest AIC and cross-validation scores and its fitted parameter values are of the same orders of magnitude as literature estimates. Maps ofK FLT and K FLT-2tiss are strongly correlated (r = 0.85) and also correlate closely with SUV maps (r = 0.72 for K FLT-2tiss , 0.64 for K FLT ). Phosphorylation rate-constant maps are moderately correlated with flux maps (r = 0.48 for k 3-2tiss vs K FLT-2tiss and r = 0.68 for k 5 vs K FLT ); however, neither phosphorylation

  3. Dek overexpression in murine epithelia increases overt esophageal squamous cell carcinoma incidence (United States)

    Cimperman, Katherine A.; Haas, Sarah R.; Guasch, Geraldine; Waclaw, Ronald R.; Komurov, Kakajan; Lane, Adam; Wikenheiser-Brokamp, Kathryn A.


    Esophageal cancer occurs as either squamous cell carcinoma (ESCC) or adenocarcinoma. ESCCs comprise almost 90% of cases worldwide, and recur with a less than 15% five-year survival rate despite available treatments. The identification of new ESCC drivers and therapeutic targets is critical for improving outcomes. Here we report that expression of the human DEK oncogene is strongly upregulated in esophageal SCC based on data in the cancer genome atlas (TCGA). DEK is a chromatin-associated protein with important roles in several nuclear processes including gene transcription, epigenetics, and DNA repair. Our previous data have utilized a murine knockout model to demonstrate that Dek expression is required for oral and esophageal SCC growth. Also, DEK overexpression in human keratinocytes, the cell of origin for SCC, was sufficient to cause hyperplasia in 3D organotypic raft cultures that mimic human skin, thus linking high DEK expression in keratinocytes to oncogenic phenotypes. However, the role of DEK over-expression in ESCC development remains unknown in human cells or genetic mouse models. To define the consequences of Dek overexpression in vivo, we generated and validated a tetracycline responsive Dek transgenic mouse model referred to as Bi-L-Dek. Dek overexpression was induced in the basal keratinocytes of stratified squamous epithelium by crossing Bi-L-Dek mice to keratin 5 tetracycline transactivator (K5-tTA) mice. Conditional transgene expression was validated in the resulting Bi-L-Dek_K5-tTA mice and was suppressed with doxycycline treatment in the tetracycline-off system. The mice were subjected to an established HNSCC and esophageal carcinogenesis protocol using the chemical carcinogen 4-nitroquinoline 1-oxide (4NQO). Dek overexpression stimulated gross esophageal tumor development, when compared to doxycycline treated control mice. Furthermore, high Dek expression caused a trend toward esophageal hyperplasia in 4NQO treated mice. Taken together, these

  4. 2ΔH(D) and 1ΔN(D) isotope effects on nuclear shielding of ammonium ions in complexes with crown ethers and cryptands

    International Nuclear Information System (INIS)

    Hansen, P.E.; Hansen, Aa.E.; Lycka, A.; Buvari-Barcza, A.


    One-bond deuterium isotope effects on nitrogen nuclear shielding, 1 ΔN(D) and two-bond isotope effects at 1 H nuclear shielding, 2 ΔH(D), have been investigated in a series of inclusion complexes. The hosts comprise, SC-24, [2.2.2], [2.2.1], [2.2.1] D, [2.2] DD, K5, 18-crown-6 and 18-crown-6 tetracarboxylic acid. The structure of the host [2.2.1] is discussed based on isotope effects and 1 H chemical shifts, and an interesting exchange reaction is observed between CDCl 3 and ammonium ions in the [2.2.1] complex. The counterion dependence is shown to be zero for ammonium ions fully included in cages such as SC-24 and [2.2.2] and for 18-C-6(COOH) 4 . For cryptands and podands such as [2.2.1] and K5 a weak counterion dependence of 2 ΔH(D) opposite to that found for ammonium ions in water is observed, while 1 ΔN(D) and 15 N chemical shifts depend very strongly on the counterions, as also found for ammonium ions in water solution. The anilinium ion shows effects similar to those observed for the ammonium ion. 1 ΔN(D) isotope effects correlate well with δN and 2 ΔH(D) correlate with δNH. The correlation between 1 ΔN(D) and 2 ΔH(D) is different for ammonium ions in water and ions included in crowns, cryptands or podands. This shows that different mechanisms are operating. The 2 ΔH(D) isotope effects and δNH chemical shifts depend on the distance to the nearest acceptor (oxygen or nitrogen), and they also depend on the type of acceptor. 2 ΔH(D) isotope effects are found to be a good gauge of N ... N or N ... O distances of the inclusion complexes. The 2 ΔH(D) isotope effects vary with temperature, but the dramatic changes in the 1 H NMR spectra of the host are not reflected in the isotope effects. The one-bond couplings, 1 J(N,H), correlate with 2 ΔH(D). The variations are much larger for the cryptands than found in water solutions. (au) (50 refs.)

  5. Optimasi Lahan dengan Sistem Tumpangsari Jagung Manis (Zea mays saccharata, Strurt dan Kangkung Sutera (Ipomea reptans di Pekanbaru

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    Surtinah Surtinah


    Full Text Available Penelitian dilaksanakan secara eksperimen dengan menggunakan rancangan lingkungan acak lengkap dengan 3 kali ulangan, dan rancangan perlakuan yaitu waktu tanam kangkung sebagai tanaman tumpangsari pada budidaya jagung manis ( K yang terdiri dari : k1 =  waktu tanam bersamaan; k2 = waktu tanam kangkung 1 minggu setelah tanam jagung manis; k3 = waktu tanam kangkung 2 minggu setelah tanam jagung manis; k4 = waktu tanam kangkung 3 minggu setelah tanam jagung manis; k5= penanaman jagung manis tunggal dengan waktu tanam = k1; k6 = penanaman kangkung tunggal dengan waktu tanam = k1.      Hasil penelitian menunjukan bahwa kangkung sutera dapat ditanam bersamaan dengan waktu tanam jagungmanis, sampai dengan tiga minggu setelah tanam jagung manis.  Hasil jagung manis tidak dipengaruhi oleh waktu tanam kangkung sutera yang berbeda. Nilai Kesetaraan Lahan pada sistem tumpangsari jagung manis dengan kangkung sutera adalah 1,83. Indeks Persaingan antara jagung manis dan kangkung sutera adalah 1,73. Kata kunci : Jagung manis, Kangkung sutera, Tumpangsari, NKL, Indeks Persaingan. Research conducted experiments using a completely randomized design environment with three replications, and design of treatment that is the time of planting silk kale as secondary crops to the cultivation of sweet corn (K consisting of: k1 = planting at the same time; k2 = time of planting silk kale 1 week after planting sweet corn; k3 = time of planting silk kale 2 weeks after planting sweet corn; k4 = time of planting silk kale 3 weeks after planting sweet corn; K5 = single planting sweet corn planting time = k1; k6 = single silk kale planting time = k1. The results showed that silk kale can be planted together with sweet corn planting time, up to three weeks after planting sweet corn. Results of sweet corn is not influenced by the timing of planting different silk kale. Land values in the cropping system Equality sweet corn with silk kale is 1.83. Competition index between

  6. Socio-demographic factors and psychological distress in Indigenous and non-Indigenous Australian adults aged 18-64 years: analysis of national survey data

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    Cunningham Joan


    Full Text Available Abstract Background Indigenous Australians are known to be at greater risk of morbidity and mortality from mental health related conditions, but most available data relate to the use of mental health services, and little is known about other aspects of social and emotional wellbeing. Using the first available nationally representative data, we examined the prevalence and patterning of psychological distress among Indigenous Australian adults and compared these with corresponding data from the non-Indigenous population. Methods The analysis used weighted data on psychological distress, as measured by a modified Kessler Psychological Distress score (K5, and a range of socio-demographic measures for 5,417 Indigenous and 15,432 non-Indigenous adults aged 18-64 years from two nationally representative surveys. Very high psychological distress (VHPD was defined as a K5 score ≥ 15 (possible range = 5-25. Results Indigenous adults were about three times more likely than non-Indigenous adults to be classified with VHPD: 14.5% (95% confidence interval (CI 12.9-16.0% versus 5.5% (95% CI 5.0-5.9%. After adjusting for age, most socio-demographic variables were significantly associated with VHPD in both populations, although the relative odds were generally larger among non-Indigenous people. Indigenous people in remote areas had a lower prevalence of VHPD than their non-remote counterparts, and only marital status, main language, and food insecurity were significantly associated with VHPD in remote areas. Conclusions Higher absolute levels of VHPD combined with smaller socio-demographic gradients in the Indigenous population suggest the importance of risk factors such as interpersonal racism, marginalization and dispossession, chronic stress and exposure to violence that are experienced by Indigenous Australians with common and/or cross-cutting effects across the socioeconomic spectrum. The lower prevalence of VHPD and lack of association with many socio

  7. Synthesis, Characterization, and Antimicrobial Studies of Novel Series of 2,4-Bis(hydrazino-6-substituted-1,3,5-triazine and Their Schiff Base Derivatives

    Directory of Open Access Journals (Sweden)

    Hessa H. Al-Rasheed


    Full Text Available The present work represents the synthesis, characterization, and antimicrobial studies of novel series of 2,4-bis(hydrazino-6-substituted-1,3,5-triazine and their Schiff base derivatives. IR, NMR (H1 and C13, elemental analysis, and LC-MS characterized the prepared compounds. The biological activity of the target products was evaluated as well. Twenty-two of the prepared compounds were selected according to their solubility in aqueous DMSO. Only eight compounds showed good activity against the selected pathogenic bacteria and did not show antagonistic effect against fungus Candida albicans. Two compounds 4k and 5g have wide-range effect presently in Gram-positive and Gram-negative bacteria while other compounds (4f, 4i, 4m, 5d, 6i, and 6h showed specific effect against the Gram-negative or Gram-positive bacteria. The minimum inhibitory concentration (MIC, μg/mL of 4f, 4i, 4k, and 6h compounds against Streptococcus mutans was 62.5 μg/mL, 100 μg/mL, 31.25 μg/mL, and 31.25 μg/mL, respectively. The MIC of 4m, 4k, 5d, 5g, and 6h compounds against Staphylococcus aureus was 62.5 μg/mL, 31.25 μg/mL, 31.25 μg/mL, 100 μg/mL, and 62.5 μg/mL, respectively. The MIC of 4k, 5g, and 6i compounds against Salmonella typhimurium was 31.25 μg/mL, 100 μg/mL, and 62.5 μg/mL, respectively. The MIC of 6i compound against Escherichia coli was 62.5 μg/mL.

  8. Selective Oxidation of a 0.1C-6Mn-2Si Third Generation Advanced High-Strength Steel During Dew-Point Controlled Annealing (United States)

    Pourmajidian, Maedeh; McDermid, Joseph R.


    The present study investigates the selective oxidation of a 0.1C-6Mn-2Si medium-Mn advanced high-strength steel during austenization annealing heat treatments as a function of process atmosphere oxygen partial pressure and annealing time. It was determined that the surface oxide growth kinetics followed a parabolic rate law with the minimum rate belonging to the lowest oxygen partial pressure atmosphere at a dew point of 223 K (- 50 °C). The chemistry of the surface and subsurface oxides was studied using STEM + EELS on the sample cross sections, and it was found that the surface oxides formed under the 223 K (- 50 °C) dew-point atmosphere consisted of a layered configuration of SiO2, MnSiO3, and MnO, while in the case of the higher pO2 process atmospheres, only MnO was detected at the surface. Consistent with the Wagner calculations, it was shown that the transition to internal oxidation for Mn occurred under the 243 K (- 30 °C) and 278 K (+ 5 °C) dew-point atmospheres. However, the predictions of the external to internal oxidation for Si using the Wagner model did not correlate well with the experimental findings nor did the predictions of the Mataigne et al. model for multi-element alloys. Investigations of the internal oxide network at the grain boundaries revealed a multilayer oxide structure composed of amorphous SiO2 and crystalline MnSiO3, respectively, at the oxide core and outer shell. A mechanism for the formation of the oxide morphologies observed, based on kinetic and thermodynamic factors, was proposed. It is expected that only the fine and nodule-like MnO oxides formed on the surface of the samples annealed under the 278 K (+ 5 °C) dew-point process atmosphere for 60 and 120 seconds are sufficiently thin and of the desired dispersed morphology to promote reactive wetting by the molten galvanizing bath.

  9. Genes associated with pathogenicity of avian Escherichia coli (APEC isolated from respiratory cases of poultry Genes associados à patogenicidade de Escherichia coli patogênica para aves (APEC isoladas de frangos de corte com sintomatologia clínica respiratória

    Directory of Open Access Journals (Sweden)

    Ana C.G.P. Rocha


    Full Text Available The virulence mechanisms of avian pathogenic Escherichia coli (APEC have been continually studied and are believed to be multi-factorial. Certain properties are primarily associated with virulent samples and have been identified in avian isolates. In this study a total of 61 E. coli, isolates from chicken flocks with respiratory symptomatology, were probed by Polimerase Chain Reation (PCR for the presence of genes responsible for the adhesion capacity, P fimbria (papC e F11 fimbria (felA, colicin production (cvaC, aerobactin presence (iutA, serum resistance (iss, temperature-sensitive hemagglutinin (tsh, and presence of K1 and K5 capsular antigens (kpsII. The iss gene was detected in 73,8%, tsh in 55,7%, iutA in 45,9%, felA in 39,3%, papC in 24,3%, cvaC in 23% and kpsII in18%.Os mecanismos de virulência das amostras de Escherichia coli potencialmente patogênicas para aves (APEC têm sido continuamente estudados e acredita-se ser multifatorial. Certas propriedades são associadas primariamente a amostras virulentas e vêm sendo identificadas em amostras de E. coli isoladas de aves. Neste estudo um total de 61 amostras de E. coli, isoladas de frangos de corte com problemas respiratórios, foram testadas através da Reação em Cadeia da Polimerase (PCR, para a presença dos genes responsáveis pela capacidade de adesão, fimbria P (papC e fimbria F11 (felA, produção de colicinas (cvaC, presença de aerobactina (iutA, resistência sérica (iss, hemaglutinina temperatura sensível (tsh e presença de dos antígenos capsulares K1 e K5 (kpsII. O gene iss foi detectado em 73,8%, tsh em 55,7%, iutA em 45,9%, felA em 39,3%, papC em 24,3%, cvaC em 23% e kpsII em 18%.

  10. Assessment of two theoretical methods to estimate potentiometric titration curves of peptides: comparison with experiment. (United States)

    Makowska, Joanna; Bagiñska, Katarzyna; Makowski, Mariusz; Jagielska, Anna; Liwo, Adam; Kasprzykowski, Franciszek; Chmurzyñski, Lech; Scheraga, Harold A


    We compared the ability of two theoretical methods of pH-dependent conformational calculations to reproduce experimental potentiometric titration curves of two models of peptides: Ac-K5-NHMe in 95% methanol (MeOH)/5% water mixture and Ac-XX(A)7OO-NH2 (XAO) (where X is diaminobutyric acid, A is alanine, and O is ornithine) in water, methanol (MeOH), and dimethyl sulfoxide (DMSO), respectively. The titration curve of the former was taken from the literature, and the curve of the latter was determined in this work. The first theoretical method involves a conformational search using the electrostatically driven Monte Carlo (EDMC) method with a low-cost energy function (ECEPP/3 plus the SRFOPT surface-solvation model, assumming that all titratable groups are uncharged) and subsequent reevaluation of the free energy at a given pH with the Poisson-Boltzmann equation, considering variable protonation states. In the second procedure, molecular dynamics (MD) simulations are run with the AMBER force field and the generalized Born model of electrostatic solvation, and the protonation states are sampled during constant-pH MD runs. In all three solvents, the first pKa of XAO is strongly downshifted compared to the value for the reference compounds (ethylamine and propylamine, respectively); the water and methanol curves have one, and the DMSO curve has two jumps characteristic of remarkable differences in the dissociation constants of acidic groups. The predicted titration curves of Ac-K5-NHMe are in good agreement with the experimental ones; better agreement is achieved with the MD-based method. The titration curves of XAO in methanol and DMSO, calculated using the MD-based approach, trace the shape of the experimental curves, reproducing the pH jump, while those calculated with the EDMC-based approach and the titration curve in water calculated using the MD-based approach have smooth shapes characteristic of the titration of weak multifunctional acids with small differences

  11. Epidemiologic Features and Prognosis of Patients Who Was Diagnosed Having Type 2 Diabetes Mellitus and Applied to a Community Health Center

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    Sevilay Hindistan


    Full Text Available AIM: The study was designed to investigate the prognosis and the epidemiologic features of people who was diagnosed having type 2 DM and Applied to the Community Health Center. METHOD: This study which was carried out at Catak Health Branch of Trabzon Erdogdu Health Center in April, May, and June in 2008 is a descriptive and cross-sectional field study. All of the patients with Type 2 DM diagnosis who came to the Health Center for routine control (55 people were involved in the study. The aim of the study was explained to each participant in the study and their oral consent was taken. As the data collection instrument, diabetes surveillance form which includes of socio-demographic features, risk factors, blood sugar diagnosis criteria, body mass index, chronic complications and foot examination was used. Diabetes surveillance form was shaped by modifying the diabetes diagnosis form which was structured by Erdogan and Nahcivan (1999. The data obtained were evaluated through number and percentage distribution, mean, chi square test, correlation and variant analyses techniques. RESULTS: Age mean of the patients was 59.4±10.7 and 85.5% of them was female. Average diagnosis age was 53.2±12.5, average diabetes year was 6,6±6,6. 45.5% of the patients were obese, 12.7% of them morbid obese and diabetes history in the family was about 54.5%. It was found that 56.3% patients had blood glucose level was 140 mg/dl and 54.5% of the patients’ HbA1c level were higher than 7%. When lipid parameter levels of the patients were examined, it was seen that HDL cholesterol level was (E<35,K<45 for 52.7% of the patients and that was lower than the target treatment level; LDL cholesterol level was 100mg/dl for 76.4%; and total cholesterol level was higher than 200 mg/dl 49.1% of the patients. A statistically significant positive correlation was found between diabetes year of the patients and HbA1c level (r=0.291, p=0.031. 8.6 had % neuropathy, 1.7% of them had

  12. Bacterial effector binding to ribosomal protein s3 subverts NF-kappaB function.

    Directory of Open Access Journals (Sweden)

    Xiaofei Gao


    as an AP-1-dependent reporter. We identified a region of NleH1 (N40-K45 that is at least partially responsible for the inhibitory activity of NleH1 toward RPS3. Deleting nleH1 from E. coli O157:H7 produced a hypervirulent phenotype in a gnotobiotic piglet model of Shiga toxin-producing E. coli infection. We suggest that NleH may disrupt host innate immune responses by binding to a cofactor of host transcriptional complexes.

  13. Integration of human adipocyte chromosomal interactions with adipose gene expression prioritizes obesity-related genes from GWAS. (United States)

    Pan, David Z; Garske, Kristina M; Alvarez, Marcus; Bhagat, Yash V; Boocock, James; Nikkola, Elina; Miao, Zong; Raulerson, Chelsea K; Cantor, Rita M; Civelek, Mete; Glastonbury, Craig A; Small, Kerrin S; Boehnke, Michael; Lusis, Aldons J; Sinsheimer, Janet S; Mohlke, Karen L; Laakso, Markku; Pajukanta, Päivi; Ko, Arthur


    Increased adiposity is a hallmark of obesity and overweight, which affect 2.2 billion people world-wide. Understanding the genetic and molecular mechanisms that underlie obesity-related phenotypes can help to improve treatment options and drug development. Here we perform promoter Capture Hi-C in human adipocytes to investigate interactions between gene promoters and distal elements as a transcription-regulating mechanism contributing to these phenotypes. We find that promoter-interacting elements in human adipocytes are enriched for adipose-related transcription factor motifs, such as PPARG and CEBPB, and contribute to heritability of cis-regulated gene expression. We further intersect these data with published genome-wide association studies for BMI and BMI-related metabolic traits to identify the genes that are under genetic cis regulation in human adipocytes via chromosomal interactions. This integrative genomics approach identifies four cis-eQTL-eGene relationships associated with BMI or obesity-related traits, including rs4776984 and MAP2K5, which we further confirm by EMSA, and highlights 38 additional candidate genes.

  14. Survey of Differentially Methylated Promoters in Prostate Cancer Cell Lines

    Directory of Open Access Journals (Sweden)

    Yipeng Wang


    Full Text Available DNA methylation, copy number in the genomes of three immortalized prostate epithelial, five cancer cell lines (LNCaP, PC3, PC3M, PC3M-Pro4, PC3MLN4 were compared using a microarray-based technique. Genomic DNA is cut with a methylation-sensitive enzyme Hpall, followed by linker ligation, polymerase chain reaction (PCR amplification, labeling, hybridization to an array of promoter sequences. Only those parts of the genomic DNA that have unmethylated restriction sites within a few hundred base pairs generate PCR products detectable on an array. Of 2732 promoter sequences on a test array, 504 (18.5% showed differential hybridization between immortalized prostate epithelial, cancer cell lines. Among candidate hypermethylated genes in cancer-derived lines, there were eight (CD44, CDKN1A, ESR1, PLAU, RARB, SFN, TNFRSF6, TSPY previously observed in prostate cancer, 13 previously known methylation targets in other cancers (ARHI, bcl-2, BRCA1, CDKN2C, GADD45A, MTAP, PGR, SLC26A4, SPARC, SYK, TJP2, UCHL1, WIT-1. The majority of genes that appear to be both differentially methylated, differentially regulated between prostate epithelial, cancer cell lines are novel methylation targets, including PAK6, RAD50, TLX3, PIR51, MAP2K5, INSR, FBN1, GG2-1, representing a rich new source of candidate genes used to study the role of DNA methylation in prostate tumors.

  15. The pro-angiogenic properties of multi-functional bioactive glass composite scaffolds

    KAUST Repository

    Gerhardt, Lutz Christian; Widdows, Kate L.; Erol, Melek M.; Burch, Charles W.; Sanz-Herrera, José A.; Ochoa, Ignacio; Stä mpfli, Rolf; Roqan, Iman S.; Gabe, Simon M.; Ansari, Tahera I.; Boccaccini, Aldo R.


    The angiogenic properties of micron-sized (m-BG) and nano-sized (n-BG) bioactive glass (BG) filled poly(D,L lactide) (PDLLA) composites were investigated. On the basis of cell culture work investigating the secretion of vascular endothelial growth factor (VEGF) by human fibroblasts in contact with composite films (0, 5, 10, 20 wt %), porous 3D composite scaffolds, optimised with respect to the BG filler content capable of inducing angiogenic response, were produced. The in vivo vascularisation of the scaffolds was studied in a rat animal model and quantified using stereological analyses. The prepared scaffolds had high porosities (81-93%), permeability (k = 5.4-8.6 × 10-9 m2) and compressive strength values (0.4-1.6 MPa) all in the range of trabecular bone. On composite films containing 20 wt % m-BG or n-BG, human fibroblasts produced 5 times higher VEGF than on pure PDLLA films. After 8 weeks of implantation, m-BG and n-BG containing scaffolds were well-infiltrated with newly formed tissue and demonstrated higher vascularisation and percentage blood vessel to tissue (11.6-15.1%) than PDLLA scaffolds (8.5%). This work thus shows potential for the regeneration of hard-soft tissue defects and increased bone formation arising from enhanced vascularisation of the construct. © 2011 Elsevier Ltd.

  16. Differential patterns of histone acetylation in inflammatory bowel diseases

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    Adcock Ian M


    Full Text Available Abstract Post-translational modifications of histones, particularly acetylation, are associated with the regulation of inflammatory gene expression. We used two animal models of inflammation of the bowel and biopsy samples from patients with Crohn's disease (CD to study the expression of acetylated histones (H 3 and 4 in inflamed mucosa. Acetylation of histone H4 was significantly elevated in the inflamed mucosa in the trinitrobenzene sulfonic acid model of colitis particularly on lysine residues (K 8 and 12 in contrast to non-inflamed tissue. In addition, acetylated H4 was localised to inflamed tissue and to Peyer's patches (PP in dextran sulfate sodium (DSS-treated rat models. Within the PP, H3 acetylation was detected in the mantle zone whereas H4 acetylation was seen in both the periphery and the germinal centre. Finally, acetylation of H4 was significantly upregulated in inflamed biopsies and PP from patients with CD. Enhanced acetylation of H4K5 and K16 was seen in the PP. These results demonstrate that histone acetylation is associated with inflammation and may provide a novel therapeutic target for mucosal inflammation.

  17. Nitrogen-Rich Energetic Metal-Organic Framework: Synthesis, Structure, Properties, and Thermal Behaviors of Pb(II Complex Based on N,N-Bis(1H-tetrazole-5-yl-Amine

    Directory of Open Access Journals (Sweden)

    Qiangqiang Liu


    Full Text Available The focus of energetic materials is on searching for a high-energy, high-density, insensitive material. Previous investigations have shown that 3D energetic metal–organic frameworks (E-MOFs have great potential and advantages in this field. A nitrogen-rich E-MOF, Pb(bta·2H2O [N% = 31.98%, H2bta = N,N-Bis(1H-tetrazole-5-yl-amine], was prepared through a one-step hydrothermal reaction in this study. Its crystal structure was determined through single-crystal X-ray diffraction, Fourier transform infrared spectroscopy, and elemental analysis. The complex has high heat denotation (16.142 kJ·cm−3, high density (3.250 g·cm−3, and good thermostability (Tdec = 614.9 K, 5 K·min−1. The detonation pressure and velocity obtained through theoretical calculations were 43.47 GPa and 8.963 km·s−1, respectively. The sensitivity test showed that the complex is an impact-insensitive material (IS > 40 J. The thermal decomposition process and kinetic parameters of the complex were also investigated through thermogravimetry and differential scanning calorimetry. Non-isothermal kinetic parameters were calculated through the methods of Kissinger and Ozawa-Doyle. Results highlighted the nitrogen-rich MOF as a potential energetic material.

  18. Metallicity and ultraviolet excesses of late main sequence stars

    International Nuclear Information System (INIS)

    Suchkov, A.A.; Marsakov, V.A.; Shevelev, Yu.G.


    The comparison of the characteristics of ultraviolet (UV) excesses δ(U-B) and metallicity [Fe/H] distributions of F, G, and K dwarfs reveals a number of discrepancies. It is shown that they can be eliminated if we assume that UV excesses of K and late G dwarfs, and [Fe/H] values from detailed analysis for F dwarfs are underestimated. Such an assumption enables to account for low values of for F, K and late G dwarfs, and for the difference of the free terms in the metallicity - UV-excess relation for these stars as compared to early G dwarfs. In this case the F5-F9 dwarfs turn out to be more metal-rich (by 0.1 in [Fe/H]) than G and K dwarfs, and the metallicity of the Hyades cluster turns out to be larger than the solar one, [Fe/H] Hyades =+0.1. The ''conditional'' metallicity - UV-excess calibrations are obtained for four groups of main-sequence stars: F5-F9, G0-G4, G5-G9, K0-K5

  19. The production and activity test of cellulases using bagasse substrate on Aspergillus niger isolated from Clove field, Kare, Madiun (United States)

    Ardhi, Muh. Waskito; Sulistyarsi, Ani; Pujiati


    Aspergillus sp is a microorganism which has a high ability to produce cellulase enzymes. In producing Cellulase enzymes requires appropriate concentration and incubation time to obtain optimum enzyme activity. This study aimed to determine the effect of inoculum concentration and incubation time towards production and activity of cellulases from Aspergillus sp substrate bagasse. This research used experiments method; completely randomized design with 2 factorial repeated 2 times. The treatment study include differences inoculum (K) 5% (K1), 15% (K2) 25%, (K3) and incubation time (F) that is 3 days (F1), 6 days (F2), 9 days (F3), 12 days (F4). The data taken from the treatment are glucose reduction and protein levels of crude cellulase enzyme activity that use Nelson Somogyi and Biuret methods. Analysis of variance ANOVA data used two paths with significance level of 5% then continued with LSD test. The results showed that: Fhit>Ftab. Thus, there is effect of inoculum concentrations and incubation time toward activity of crude cellulases of Aspergillus sp. The highest glucose reduction of treatment is K3F4 (concentration of inoculum is 25% with 12 days incubation time) amount 12.834 g / ml and the highest protein content is K3F4 (concentration of inoculum is 25% with with 12 days incubation time) amount 0.740 g / ml.

  20. KüFA safety testing of HTR fuel pebbles irradiated in the High Flux Reactor in Petten

    Energy Technology Data Exchange (ETDEWEB)

    Seeger, O., E-mail: [European Commission, Joint Research Centre (JRC), Institute for Transuranium Elements (ITU), Safety of Irradiated Nuclear Materials Unit, Postfach 2340, 76125 Karlsruhe (Germany); Laurie, M., E-mail: [European Commission, Joint Research Centre (JRC), Institute for Transuranium Elements (ITU), Safety of Irradiated Nuclear Materials Unit, Postfach 2340, 76125 Karlsruhe (Germany); Abjani, A. El; Ejton, J.; Boudaud, D.; Freis, D.; Carbol, P.; Rondinella, V.V. [European Commission, Joint Research Centre (JRC), Institute for Transuranium Elements (ITU), Safety of Irradiated Nuclear Materials Unit, Postfach 2340, 76125 Karlsruhe (Germany); Fütterer, M. [European Commission, Joint Research Centre (JRC), Institute for Energy and Transport (IET), Nuclear Reactor Integrity Assessment and Knowledge Management Unit, PO Box 2, 1755 ZG Petten (Netherlands); Allelein, H.-J. [Lehrstuhl für Reaktorsicherheit und -technik an der RWTH Aachen, Kackertstraße 9, 52072 Aachen (Germany)


    The Cold Finger Apparatus (KühlFinger-Apparatur—KüFA) in operation at JRC-ITU is designed to experimentally scrutinize the effects of Depressurization LOss of Forced Circulation (D-LOFC) accident scenarios on irradiated High Temperature Reactor (HTR) fuel pebbles. Up to 1600 °C, the reference maximum temperature for these accidents, high-quality German HTR fuel pebbles have already demonstrated a small fission product release. This paper discusses and compares the releases obtained from KüFA-testing the pebbles HFR-K5/3 and HFR-EU1/3, which were both irradiated in the High Flux Reactor (HFR) in Petten. We present the time-dependent fractional release of the volatile fission product {sup 137}Cs as well as the fission gas {sup 85}Kr for both pebbles. For HFR-EU1/3 the isotopes {sup 134}Cs and {sup 154}Eu as well as the shorter-lived {sup 110m}Ag have also been measured. A detailed description of the experimental setup and its accuracy is given. The data for the recently tested pebbles is discussed in the context of previous results.

  1. Fitoremediasi Tanaman Akar Wangi (Vetiver zizanioides Terhadap Tanah Tercemar Logam Kadmium (Cd Pada Lahan TPA Tamangapa Antang Makassar

    Directory of Open Access Journals (Sweden)

    Alfia Patandungan


    Full Text Available One example of soil contamination on land landfill is (TPA Makassar. The method of prevention is phytoremediation of the contaminated land. This study examined the ability of the plant will vetiver (Vetiver zizanioides in reducing the levels of Cd in the soil. To increase the potential of these plants to remediate Cd, the soil where the plants grow is combined with compost in which bacteria within the compost might improve the absorption of Cd. Planting medium used is pure soil and compost from Tamangapa Makassar. Research carried out for 28 days with a variation of the study. The composition of the media that the contaminated soil (TT metal kadmium  (Cd  and compost (K with a ratio of 100% (TT: 0 K, 5 (TT: 1 (K, 4.5 (TT: 1.5 (K and 4 (TT: 2 (K. The results shwed that vetiver plants were able to absorb Cd of 0,298 mg/Kg so it can be concluded that the composition of the planting medium with a combination of compost less significant because the combination of the contaminated soil with compost are less precise in  helping vetiver plants accumulate or reduce metal pollution cadmium in  contaminated soil Tamangapa Antang Makassar.

  2. Results of candidemia treatment in children with hematologic malignancies: single center experience

    Directory of Open Access Journals (Sweden)

    I. I. Kalinina


    Full Text Available Candidemia is one of the most serious infectious complications in children with hematological malignancies and has a high morta lity rate.Seven-year experience of candidemia diagnosis and therapy in patients with various hematologic malignancies w as analyzed. Candidemia registered in 37 patients (AML and MDS — 14, ALL — 10, solid tumors — 5, histocytic syndromes — 4, AA — 3, other non-malignancy diseases— 2. C. non-albicans (36 isolates from 32 patients was common cause of, while C. albicans isolated in 5 patients (8 strains. Antifungal prophylactic therapy was applied to 31 patients. 22 patients at the time of candidemia have neutropenia (< 0.5 × 10 9/l. Main clinical manifestations were febrile fever (100 % cases and pneumonia (21.6 % cases. Less frequent multiorgan failure (8.1 %, septic shoc k (5.4 %, chronic disseminated candidiasis (5.4 % and meningitis (2.7 % were registered. All patients received antifungal therapy (monotherapy — 17, combination therapy — 20. Central venous catheter removed in 21 patients. In 14 patients hematopoietic recovery w as registered, none of these patients died, while from group of patients without hematopoietic recovery 6 patients died (p = 0.0001. Recurrent candidemia episodes were seen in 4 patients. Overall survival was 0.37 ± 0.09.

  3. A Binary System in the Hyades Cluster Hosting a Neptune-Sized Planet (United States)

    Feinstein, Adina; Ciardi, David; Crossfield, Ian; Schlieder, Joshua; Petigura, Erik; David, Trevor J.; Bristow, Makennah; Patel, Rahul; Arnold, Lauren; Benneke, Björn; Christiansen, Jessie; Dressing, Courtney; Fulton, Benjamin; Howard, Andrew; Isaacson, Howard; Sinukoff, Evan; Thackeray, Beverly


    We report the discovery of a Neptune-size planet (Rp = 3.0Rearth) in the Hyades Cluster. The host star is in a binary system, comprising a K5V star and M7/8V star with a projected separation of 40 AU. The planet orbits the primary star with an orbital period of 17.3 days and a transit duration of 3 hours. The host star is bright (V = 11.2, J = 9.1) and so may be a good target for precise radial velocity measurements. The planet is the first Neptune-sized planet to be found orbiting in a binary system within an open cluster. The Hyades is the nearest star cluster to the Sun, has an age of 625-750 Myr, and forms one of the fundamental rungs in the distance ladder; understanding the planet population in such a well-studied cluster can help us understand and set contraints on the formation and evolution of planetary systems.

  4. Analysis of Major Item Redistribution/Substitution Policies. Volume 2 (United States)


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  5. Efficiency of the SASS4 rapid bioassessment protocol in determining river health: A case study on the Mhlathuze River, KwaZulu-Natal, South Africa

    CSIR Research Space (South Africa)

    Vos, P


    Full Text Available the labour and time factors confronting freshwater ecologists, since the number of habitats examined and the replicated sample units taken per site are reduced (Chutter, 1994). Dallas (1995, 1997) addressed some of the uncertainties associated with SASS4... 3b 4l 4a 4b 5l 5a 5b 6l 6a 6b I II III VI V Number of taxa ASPT values 2c 2d 2e 3c 3d 3e 4c 4d 4e 5c5d 5e 6c 6d 6e I II III IV V VI VII 2f 2g 2h 3f 3g 3h 4f 4g 4h 5f5g 5h 6g 6h I II III IV V VI 2i 2j 2k 3i 3j 3k 4i4j 4k 5i 5j 5k 6i 6j6k I II III IV V...


    Directory of Open Access Journals (Sweden)

    Nur Aisyah


    Full Text Available Penelitian ini bertujuan untuk mengetahui perbedaan kandungan vitamin C dan pH dalam sayuran bunga kol, kubis dan buncis yang diawetkan dengan kitosan cangkang bekicot selama proses penyimpanan dan untuk mengetahui berapakah konsentrasi kitosan cangkang bekicot yang paling efektif sebagai bahan pengawet sayuran tersebut. Kegiatan penelitian dilakukan melalui True Experimental Research. Tempat dan waktu penelitian dilaksanakan di Laboratorium Kimia Universitas Muhammadiyah Malang yang berlangsung pada tanggal 23 Juni – 6 Juli 2014. Rancangan penelitian yang digunakan adalah Rancangan Acak Kelompok (RAK dengan 6 perlakuan dan 4 kali ulangan pada bunga kol, kubis dan buncis yaitu C0, K0 dan B0 (Kontrol, C1, K1 dan B1 (0,5%, C2, K2 dan B2 (1%, C3, K3 dan B3(1,5%l, C4, K4 dan B4 (2%, C5, K5 dan B5 (2,5%, Analisis data menggunakan analisis varians satu arah dan uji beda jarak nyata Duncan pada taraf signifikansi 0,05. Hasil penelitian menunjukkan ada pengaruh pemberian konsentrasi kitosan cangkang bekicot (Achantina fulica terhadap perbedaan kandungan vitamin C sayuran bunga kol, kubis dan buncis dari hari per hari selama penyimpanan. Perubahan kandungan vitamin C paling kecil terjadi pada perlakuan 1,5% dan paling besar pada perlakuan kontrol. Pemberian konsentrasi kitosan cangkang bekicot (Achantina fulica 1,5% adalah yang paling efektif mempengaruhi kandungan fitamin C sayuran bunga kol, kubis dan buncis. Hasil penelitian diaplikasikan pada perencanaan pembelajaran SMA kelas XII materi bioteknologi

  7. The use of moving bed bio-reactor to laundry wastewater treatment (United States)

    Bering, Sławomira; Mazur, Jacek; Tarnowski, Krzysztof; Janus, Magdalena; Mozia, Sylwia; Waldemar Morawski, Antoni


    Large laboratory scale biological treatment test of industrial real wastewater, generated in industrial big laundry, has been conducted in the period of May 2016-August 2016. The research aimed at selection of laundry wastewater treatment technology included tests of two-stage Moving Bed Bio Reactor (MBBR), with two reactors filled with carriers Kaldnes K5 (specific area - 800 m2/m3), have been realized in aerobic condition. Operating on site, in the laundry, reactors have been fed real wastewater from laundry retention tank. To the laundry wastewater, contained mainly surfactants and impurities originating from washed fabrics, a solution of urea to supplement nitrogen content and a solution of acid to correct pH have been added. Daily flow of raw wastewater Qd was equal to 0.6-0.8 m3/d. The values of determined wastewater quality indicators showed that substantial decrease of pollutants content have been reached: BOD5 by 94.7-98.1%, COD by 86.9-93.5%, the sum of anionic and nonionic surfactants by 98.7-99.8%. The quality of the purified wastewater, after star-up period, meets the legal requirements regarding the standards for wastewater discharged to the environment.

  8. Gravitational clustering of gaussian density fluctuations: The origin of hierarchy correlations, voids and QN

    International Nuclear Information System (INIS)

    Kashlinsky, A.


    We present here a theory of gravitational clustering based only on: i) The assumption that the primordial density fluctuations (PDF) were Gaussian; and ii) identifying galaxies, and groups and clusters of galaxies with regions of PDF whose turn-around time was less than the age of Universe. It is shown that 1) properties of the hierarchy thus produced depend uniquely on the power spectrum P(k) of PDF; 2) the resultant distribution of galaxies and groups and clusters is non-Gaussian; 3) two-point and higher order correlations depend in a complicated, but unique for a given P(k), way on the mass-scales of groups and clusters and, hence, can be used to constrain P(k) on scales from 1 to (50-100) h -1 Mpc on the basis of the available data; 4) expressions for the dimensionless correlation amplitudes Q N can then be computed for the hierarchy - again they depend uniquely on the mass and P(k); 5) statistics of voids in the distribution of galaxies and clusters of various richness/mass can then be used to constrain P(k). We show that the available data on correlation functions and voids are in better agreement with the n=-1 spectrum than with spectra having n>0 on large scales such as cold-dark-matter models. (orig.)


    Energy Technology Data Exchange (ETDEWEB)

    Lacy, Claud H. Sandberg [Physics Department, University of Arkansas, Fayetteville, AR 72701 (United States); Fekel, Francis C.; Muterspaugh, Matthew W. [Center of Excellence in Information Systems, Tennessee State University, Nashville, TN 37209 (United States); Pavlovski, Krešimir [Department of Physics, Faculty of Science, University of Zagreb, Bijenička cesta 32, 10000 Zagreb (Croatia); Torres, Guillermo, E-mail:, E-mail:, E-mail:, E-mail:, E-mail: [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States)


    NP Per is a well-detached, 2.2 day eclipsing binary whose components are both pre-main-sequence stars that are still contracting toward the main-sequence phase of evolution. We report extensive photometric and spectroscopic observations with which we have determined their properties accurately. Their surface temperatures are quite different: 6420 ± 90 K for the larger F5 primary star and 4540 ± 160 K for the smaller K5e star. Their masses and radii are 1.3207 ± 0.0087 solar masses and 1.372 ± 0.013 solar radii for the primary, and 1.0456 ± 0.0046 solar masses and 1.229 ± 0.013 solar radii for the secondary. The orbital period is variable over long periods of time. A comparison of the observations with current stellar evolution models from MESA indicates that the stars cannot be fit at a single age: the secondary appears significantly younger than the primary. If the stars are assumed to be coeval and to have the age of the primary (17 Myr), then the secondary is larger and cooler than predicted by current models. The H α spectral line of the secondary component is completely filled by, presumably, chromospheric emission due to a magnetic activity cycle.

  10. Hands-on-Science: Using Education Research to Construct Learner-Centered Classes (United States)

    Ludwig, R. R.; Chimonidou, A.; Kopp, S.


    Research into the process of learning, and learning astronomy, can be informative for the development of a course. Students are better able to incorporate and make sense of new ideas when they are aware of their own prior knowledge (Resnick et al. 1989; Confrey 1990), have the opportunity to develop explanations from their own experience in their own words (McDermott 1991; Prather et al. 2004), and benefit from peer instruction (Mazur 1997; Green 2003). Students in astronomy courses often have difficulty understanding many different concepts as a result of difficulties with spatial reasoning and a sense of scale. The Hands-on-Science program at UT Austin incorporates these research-based results into four guided-inquiry, integrated science courses (50 students each). They are aimed at pre-service K-5 teachers but are open to other majors as well. We find that Hands-on-Science students not only attain more favorable changes in attitude towards science, but they also outperform students in traditional lecture courses in content gains. Workshop Outcomes: Participants experienced a research-based, guided-inquiry lesson about the motion of objects in the sky and discussed the research methodology for assessing students in such a course.

  11. IR finiteness of the ghost dressing function from numerical resolution of the ghost SD equation

    International Nuclear Information System (INIS)

    Boucaud, Ph.; Leroy, J.P.; Yaouanc, A. Le; Micheli, J.; Pene, O.; RodrIguez-Quintero, J.


    We solve numerically the Schwinger-Dyson ghost equation in the Landau gauge for a given, finite at k = 0 gluon propagator (i.e. the infrared exponent of its dressing function, α gluon , is 1) and under the usual assumption of constancy of the ghost-gluon vertex ; we show that there exist two possible types of ghost dressing function solutions, as we have previously inferred from analytical considerations: one which is singular at zero momentum (the infrared exponent of its dressing function, α ghost , (We shall use α G and α F as shorthands for α gluon and α ghost respectively; let us recall that we denote the gluon by a G and the ghost by a F, for ''fantome''.) is gluon +2α ghost = 0 and has therefore α ghost = -1/2, and another one which is finite at the origin with α ghost = 0 and violates the relation. It is most important that the type of solution which is realized depends on the value of the coupling constant. There are regular ones - α F = 0 - for any coupling below some value, while there is only one singular solution - α F <0 -, obtained for a single critical value of the coupling. For all momenta k <.5 GeV where they can be trusted, our lattice data exclude neatly the singular one, and agree very well with the regular solution we obtain at a coupling constant compatible with the bare lattice value.

  12. Development of 2-(Substituted Benzylamino)-4-Methyl-1, 3-Thiazole-5-Carboxylic Acid Derivatives as Xanthine Oxidase Inhibitors and Free Radical Scavengers. (United States)

    Ali, Md Rahmat; Kumar, Suresh; Afzal, Obaid; Shalmali, Nishtha; Sharma, Manju; Bawa, Sandhya


    A series of 2-(substituted benzylamino)-4-methylthiazole-5-carboxylic acid was designed and synthesized as structural analogue of febuxostat. A methylene amine spacer was incorporated between the phenyl ring and thiazole ring in contrast to febuxostat in which the phenyl ring was directly linked with the thiazole moiety. The purpose of incorporating methylene amine was to provide a heteroatom which is expected to favour hydrogen bonding within the active site residues of the enzyme xanthine oxidase. The structure of all the compounds was established by the combined use of FT-IR, NMR and MS spectral data. All the compounds were screened in vitro for their ability to inhibit the enzyme xanthine oxidase as per the reported procedure along with DPPH free radical scavenging assay. Compounds 5j, 5k and 5l demonstrated satisfactory potent xanthine oxidase inhibitory activities with IC50 values, 3.6, 8.1 and 9.9 μm, respectively, whereas compounds 5k, 5n and 5p demonstrated moderate antioxidant activities having IC50 15.3, 17.6 and 19.6 μm, respectively, along with xanthine oxidase inhibitory activity. Compound 5k showed moderate xanthine oxidase inhibitory activity as compared with febuxostat along with antioxidant activity. All the compounds were also studied for their binding affinity in active site of enzyme (PDB ID-1N5X). © 2015 John Wiley & Sons A/S.

  13. Highly sensitive trivalent copper chelate–luminol chemiluminescence system for capillary electrophoresis chiral separation and determination of ofloxacin enantiomers in urine samples

    Directory of Open Access Journals (Sweden)

    Hao-Yue Xie


    Full Text Available A simple, fast and sensitive capillary electrophoresis (CE strategy combined with chemiluminescence (CL detection for analysis of ofloxacin (OF enantiomers was established in the present work. Sulfonated β-cyclodextrin (β-CD was used as the chiral additive being added into the running buffer of luminol–diperiodatocuprate (III (K5[Cu(HIO62], DPC chemiluminescence system. Under the optimum conditions, the proposed method was successfully applied to separation and analysis of OF enantiomers with the detection limits (S/N=3 of 8.0 nM and 7.0 nM for levofloxacin and dextrofloxacin, respectively. The linear ranges were both 0.010–100 μM. The method was utilized for analyzing OF in urine; the results obtained were satisfactory and recoveries were 89.5–110.8%, which demonstrated the reliability of this method. This approach can also be further extended to analyze different commercial OF medicines. Keywords: Ofloxacin, Chiral analysis, Sulfonated-β-CD, Capillary electrophoresis, Chemiluminescence

  14. Rate constant for the reaction SO + BrO yields SO2 + Br (United States)

    Brunning, J.; Stief, L.


    The rate of the radical-radical reaction SO + BrO yields SO2 + Br has been determined at 298 K in a discharge flow system near 1 torr pressure with detection of SO and BrO via collision-free sampling mass spectrometry. The rate constant was determined using two different methods: measuring the decay of SO radicals in the presence of an excess of BrO and measuring the decay of BrO radicals in excess SO. The results from the two methods are in reasonable agreement and the simple mean of the two values gives the recommended rate constant at 298 K, k = (5.7 + or - 2.0) x 10 to the -11th cu cm/s. This represents the first determination of this rate constant and it is consistent with a previously derived lower limit based on SO2 formation. Comparison is made with other radical-radical reactions involving SO or BrO. The reaction SO + BrO yields SO2 + Br is of interest for models of the upper atmosphere of the earth and provides a potential coupling between atmospheric sulfur and bromine chemistry.

  15. Potential of optimized NOνA for large θ13 and combined performance with a LArTPC and T2K

    International Nuclear Information System (INIS)

    Agarwalla, Sanjib Kumar; Prakash, Suprabh; Raut, Sushant K.; Sankar, S. Uma


    NOνA experiment has reoptimized its event selection criteria in light of the recently measured moderately large value of θ 13 . We study the improvement in the sensitivity to the neutrino mass hierarchy and to leptonic CP violation due to these new features. Addition of 5 years of neutrino data from T2K to NOνA more than doubles the range of δ CP for which the leptonic CP violation can be discovered, compared to stand alone NOνA. But for unfavorable values of δ CP , the combination of NOνA and T2K are not enough to provide even a 90% C.L. hint of hierarchy discovery. Therefore, we further explore the improvement in the hierarchy and CP violation sensitivities due to the addition of a 10 kt liquid argon detector placed close to NOνA site. The capabilities of such a detector are equivalent to those of NOνA in all respects. We find that combined data from 10 kt liquid argon detector (3 years of ν + 3 years of ν ¯ run), NOνA (6 years of ν + 6 years of ν ¯ run) and T2K (5 years of ν run) can give a close to 2σ hint of hierarchy discovery for all values of δ CP . With this combined data, we can achieve CP violation discovery at 95% C.L. for roughly 60% values of δ CP

  16. Atropisomeric property of 1-(2,6-difluorobenzyl)-3-[(2R)-amino-2-phenethyl]-5-(2-fluoro-3-methoxyphenyl)-6-methyluracil. (United States)

    Tucci, Fabio C; Hu, Tao; Mesleh, Michael F; Bokser, Allan; Allsopp, Eric; Gross, Timothy D; Guo, Zhiqiang; Zhu, Yun-Fei; Struthers, R Scott; Ling, Nicholas; Chen, Chen


    1-(2,6-Difluorobenzyl)-3-[(2R)-amino-2-phenethyl]-5-(2-fluoro-3-methoxyphenyl)-6-methyluracil (6), a potent and orally active antagonist of the human gonadotropin-releasing hormone receptor, exists as a pair of atropisomers in solution, which was detected by NMR spectroscopy, and separable by HPLC. In addition to a (R)-configured benzylamine, there is a second stereogenic element due to the presence of a chiral axis between the substituted 5-phenyl group and the uracil core. The rate constant of the interconversion (k = 5.07 x 10(-5) s(-1)) of these two atropisomers was determined by proton NMR analysis of a diastereoisomer-enriched sample in aqueous solution at 25 degrees C, and the corresponding Gibbs free energy DeltaG(#) of rotation barrier (97.4 kJ mol(-1)) was calculated using the Eyring equation. The diastereoisomer half-life at physiological temperature (37 degrees C) in aqueous media was estimated to be about 46 min. Copyright 2005 Wiley-Liss, Inc.

  17. Arikan and Alamouti matrices based on fast block-wise inverse Jacket transform (United States)

    Lee, Moon Ho; Khan, Md Hashem Ali; Kim, Kyeong Jin


    Recently, Lee and Hou (IEEE Signal Process Lett 13: 461-464, 2006) proposed one-dimensional and two-dimensional fast algorithms for block-wise inverse Jacket transforms (BIJTs). Their BIJTs are not real inverse Jacket transforms from mathematical point of view because their inverses do not satisfy the usual condition, i.e., the multiplication of a matrix with its inverse matrix is not equal to the identity matrix. Therefore, we mathematically propose a fast block-wise inverse Jacket transform of orders N = 2 k , 3 k , 5 k , and 6 k , where k is a positive integer. Based on the Kronecker product of the successive lower order Jacket matrices and the basis matrix, the fast algorithms for realizing these transforms are obtained. Due to the simple inverse and fast algorithms of Arikan polar binary and Alamouti multiple-input multiple-output (MIMO) non-binary matrices, which are obtained from BIJTs, they can be applied in areas such as 3GPP physical layer for ultra mobile broadband permutation matrices design, first-order q-ary Reed-Muller code design, diagonal channel design, diagonal subchannel decompose for interference alignment, and 4G MIMO long-term evolution Alamouti precoding design.

  18. Observation of the $\\Lambda_b^0 \\rightarrow J/\\psi p \\pi^-$ decay

    CERN Document Server

    Aaij, Roel; Adinolfi, Marco; Affolder, Anthony; Ajaltouni, Ziad; Akar, Simon; Albrecht, Johannes; Alessio, Federico; Alexander, Michael; Ali, Suvayu; Alkhazov, Georgy; Alvarez Cartelle, Paula; Alves Jr, Antonio; Amato, Sandra; Amerio, Silvia; Amhis, Yasmine; An, Liupan; Anderlini, Lucio; Anderson, Jonathan; Andreassen, Rolf; Andreotti, Mirco; Andrews, Jason; Appleby, Robert; Aquines Gutierrez, Osvaldo; Archilli, Flavio; Artamonov, Alexander; Artuso, Marina; Aslanides, Elie; Auriemma, Giulio; Baalouch, Marouen; Băbeanu, Alexandru-Ionut; Bachmann, Sebastian; Back, John; Badalov, Alexey; Balagura, Vladislav; Baldini, Wander; Barlow, Roger; Barschel, Colin; Barsuk, Sergey; Barter, William; Batozskaya, Varvara; Battista, Vincenzo; Bay, Aurelio; Beaucourt, Leo; Beddow, John; Bedeschi, Franco; Bediaga, Ignacio; Belogurov, Sergey; Belous, Konstantin; Belyaev, Ivan; Ben-Haim, Eli; Bencivenni, Giovanni; Benson, Sean; Benton, Jack; Berezhnoy, Alexander; Bernet, Roland; Bettler, Marc-Olivier; van Beuzekom, Martinus; Bien, Alexander; Bifani, Simone; Bird, Thomas; Bizzeti, Andrea; Bjørnstad, Pål Marius; Blake, Thomas; Blanc, Frédéric; Blouw, Johan; Blusk, Steven; Bocci, Valerio; Bondar, Alexander; Bondar, Nikolay; Bonivento, Walter; Borghi, Silvia; Borgia, Alessandra; Borsato, Martino; Bowcock, Themistocles; Bowen, Espen Eie; Bozzi, Concezio; Brambach, Tobias; van den Brand, Johannes; Bressieux, Joël; Brett, David; Britsch, Markward; Britton, Thomas; Brodzicka, Jolanta; Brook, Nicholas; Brown, Henry; Bursche, Albert; Busetto, Giovanni; Buytaert, Jan; Cadeddu, Sandro; Calabrese, Roberto; Calvi, Marta; Calvo Gomez, Miriam; Camboni, Alessandro; Campana, Pierluigi; Campora Perez, Daniel; Carbone, Angelo; Carboni, Giovanni; Cardinale, Roberta; Cardini, Alessandro; Carranza-Mejia, Hector; Carson, Laurence; Carvalho Akiba, Kazuyoshi; Casse, Gianluigi; Cassina, Lorenzo; Castillo Garcia, Lucia; Cattaneo, Marco; Cauet, Christophe; Cenci, Riccardo; Charles, Matthew; Charpentier, Philippe; Chen, Shanzhen; Cheung, Shu-Faye; Chiapolini, Nicola; Chrzaszcz, Marcin; Ciba, Krzystof; Cid Vidal, Xabier; Ciezarek, Gregory; Clarke, Peter; Clemencic, Marco; Cliff, Harry; Closier, Joel; Coco, Victor; Cogan, Julien; Cogneras, Eric; Collins, Paula; Comerma-Montells, Albert; Contu, Andrea; Cook, Andrew; Coombes, Matthew; Coquereau, Samuel; Corti, Gloria; Corvo, Marco; Counts, Ian; Couturier, Benjamin; Cowan, Greig; Craik, Daniel Charles; Cruz Torres, Melissa Maria; Cunliffe, Samuel; Currie, Robert; D'Ambrosio, Carmelo; Dalseno, Jeremy; David, Pascal; David, Pieter; Davis, Adam; De Bruyn, Kristof; De Capua, Stefano; De Cian, Michel; De Miranda, Jussara; De Paula, Leandro; De Silva, Weeraddana; De Simone, Patrizia; Decamp, Daniel; Deckenhoff, Mirko; Del Buono, Luigi; Déléage, Nicolas; Derkach, Denis; Deschamps, Olivier; Dettori, Francesco; Di Canto, Angelo; Dijkstra, Hans; Donleavy, Stephanie; Dordei, Francesca; Dorigo, Mirco; Dosil Suárez, Alvaro; Dossett, David; Dovbnya, Anatoliy; Dreimanis, Karlis; Dujany, Giulio; Dupertuis, Frederic; Durante, Paolo; Dzhelyadin, Rustem; Dziurda, Agnieszka; Dzyuba, Alexey; Easo, Sajan; Egede, Ulrik; Egorychev, Victor; Eidelman, Semen; Eisenhardt, Stephan; Eitschberger, Ulrich; Ekelhof, Robert; Eklund, Lars; El Rifai, Ibrahim; Elsasser, Christian; Ely, Scott; Esen, Sevda; Evans, Hannah Mary; Evans, Timothy; Falabella, Antonio; Färber, Christian; Farinelli, Chiara; Farley, Nathanael; Farry, Stephen; Fay, Robert; Ferguson, Dianne; Fernandez Albor, Victor; Ferreira Rodrigues, Fernando; Ferro-Luzzi, Massimiliano; Filippov, Sergey; Fiore, Marco; Fiorini, Massimiliano; Firlej, Miroslaw; Fitzpatrick, Conor; Fiutowski, Tomasz; Fontana, Marianna; Fontanelli, Flavio; Forty, Roger; Francisco, Oscar; Frank, Markus; Frei, Christoph; Frosini, Maddalena; Fu, Jinlin; Furfaro, Emiliano; Gallas Torreira, Abraham; Galli, Domenico; Gallorini, Stefano; Gambetta, Silvia; Gandelman, Miriam; Gandini, Paolo; Gao, Yuanning; Garofoli, Justin; Garra Tico, Jordi; Garrido, Lluis; Gaspar, Clara; Gauld, Rhorry; Gavardi, Laura; Gavrilov, Gennadii; Gersabeck, Evelina; Gersabeck, Marco; Gershon, Timothy; Ghez, Philippe; Gianelle, Alessio; Giani', Sebastiana; Gibson, Valerie; Giubega, Lavinia-Helena; Gligorov, V.V.; Göbel, Carla; Golubkov, Dmitry; Golutvin, Andrey; Gomes, Alvaro; Gordon, Hamish; Gotti, Claudio; Grabalosa Gándara, Marc; Graciani Diaz, Ricardo; Granado Cardoso, Luis Alberto; Graugés, Eugeni; Graziani, Giacomo; Grecu, Alexandru; Greening, Edward; Gregson, Sam; Griffith, Peter; Grillo, Lucia; Grünberg, Oliver; Gui, Bin; Gushchin, Evgeny; Guz, Yury; Gys, Thierry; Hadjivasiliou, Christos; Haefeli, Guido; Haen, Christophe; Haines, Susan; Hall, Samuel; Hamilton, Brian; Hampson, Thomas; Han, Xiaoxue; Hansmann-Menzemer, Stephanie; Harnew, Neville; Harnew, Samuel; Harrison, Jonathan; Hartmann, Thomas; He, Jibo; Head, Timothy; Heijne, Veerle; Hennessy, Karol; Henrard, Pierre; Henry, Louis; Hernando Morata, Jose Angel; van Herwijnen, Eric; Heß, Miriam; Hicheur, Adlène; Hill, Donal; Hoballah, Mostafa; Hombach, Christoph; Hulsbergen, Wouter; Hunt, Philip; Hussain, Nazim; Hutchcroft, David; Hynds, Daniel; Idzik, Marek; Ilten, Philip; Jacobsson, Richard; Jaeger, Andreas; Jalocha, Pawel; Jans, Eddy; Jaton, Pierre; Jawahery, Abolhassan; Jing, Fanfan; John, Malcolm; Johnson, Daniel; Jones, Christopher; Joram, Christian; Jost, Beat; Jurik, Nathan; Kaballo, Michael; Kandybei, Sergii; Kanso, Walaa; Karacson, Matthias; Karbach, Moritz; Karodia, Sarah; Kelsey, Matthew; Kenyon, Ian; Ketel, Tjeerd; Khanji, Basem; Khurewathanakul, Chitsanu; Klaver, Suzanne; Kochebina, Olga; Kolpin, Michael; Komarov, Ilya; Koopman, Rose; Koppenburg, Patrick; Korolev, Mikhail; Kozlinskiy, Alexandr; Kravchuk, Leonid; Kreplin, Katharina; Kreps, Michal; Krocker, Georg; Krokovny, Pavel; Kruse, Florian; Kucewicz, Wojciech; Kucharczyk, Marcin; Kudryavtsev, Vasily; Kurek, Krzysztof; Kvaratskheliya, Tengiz; La Thi, Viet Nga; Lacarrere, Daniel; Lafferty, George; Lai, Adriano; Lambert, Dean; Lambert, Robert W; Lanciotti, Elisa; Lanfranchi, Gaia; Langenbruch, Christoph; Langhans, Benedikt; Latham, Thomas; Lazzeroni, Cristina; Le Gac, Renaud; van Leerdam, Jeroen; Lees, Jean-Pierre; Lefèvre, Regis; Leflat, Alexander; Lefrançois, Jacques; Leo, Sabato; Leroy, Olivier; Lesiak, Tadeusz; Leverington, Blake; Li, Yiming; Liles, Myfanwy; Lindner, Rolf; Linn, Christian; Lionetto, Federica; Liu, Bo; Liu, Guoming; Lohn, Stefan; Longstaff, Iain; Lopes, Jose; Lopez-March, Neus; Lowdon, Peter; Lu, Haiting; Lucchesi, Donatella; Luo, Haofei; Lupato, Anna; Luppi, Eleonora; Lupton, Oliver; Machefert, Frederic; Machikhiliyan, Irina V; Maciuc, Florin; Maev, Oleg; Malde, Sneha; Manca, Giulia; Mancinelli, Giampiero; Maratas, Jan; Marchand, Jean François; Marconi, Umberto; Marin Benito, Carla; Marino, Pietro; Märki, Raphael; Marks, Jörg; Martellotti, Giuseppe; Martens, Aurelien; Martín Sánchez, Alexandra; Martinelli, Maurizio; Martinez Santos, Diego; Martinez Vidal, Fernando; Martins Tostes, Danielle; Massafferri, André; Matev, Rosen; Mathe, Zoltan; Matteuzzi, Clara; Mazurov, Alexander; McCann, Michael; McCarthy, James; McNab, Andrew; McNulty, Ronan; McSkelly, Ben; Meadows, Brian; Meier, Frank; Meissner, Marco; Merk, Marcel; Milanes, Diego Alejandro; Minard, Marie-Noelle; Moggi, Niccolò; Molina Rodriguez, Josue; Monteil, Stephane; Morandin, Mauro; Morawski, Piotr; Mordà, Alessandro; Morello, Michael Joseph; Moron, Jakub; Morris, Adam Benjamin; Mountain, Raymond; Muheim, Franz; Müller, Katharina; Muresan, Raluca; Mussini, Manuel; Muster, Bastien; Naik, Paras; Nakada, Tatsuya; Nandakumar, Raja; Nasteva, Irina; Needham, Matthew; Neri, Nicola; Neubert, Sebastian; Neufeld, Niko; Neuner, Max; Nguyen, Anh Duc; Nguyen, Thi-Dung; Nguyen-Mau, Chung; Nicol, Michelle; Niess, Valentin; Niet, Ramon; Nikitin, Nikolay; Nikodem, Thomas; Novoselov, Alexey; O'Hanlon, Daniel Patrick; Oblakowska-Mucha, Agnieszka; Obraztsov, Vladimir; Oggero, Serena; Ogilvy, Stephen; Okhrimenko, Oleksandr; Oldeman, Rudolf; Onderwater, Gerco; Orlandea, Marius; Otalora Goicochea, Juan Martin; Owen, Patrick; Oyanguren, Maria Arantza; Pal, Bilas Kanti; Palano, Antimo; Palombo, Fernando; Palutan, Matteo; Panman, Jacob; Papanestis, Antonios; Pappagallo, Marco; Parkes, Christopher; Parkinson, Christopher John; Passaleva, Giovanni; Patel, Girish; Patel, Mitesh; Patrignani, Claudia; Pazos Alvarez, Antonio; Pearce, Alex; Pellegrino, Antonio; Pepe Altarelli, Monica; Perazzini, Stefano; Perez Trigo, Eliseo; Perret, Pascal; Perrin-Terrin, Mathieu; Pescatore, Luca; Pesen, Erhan; Petridis, Konstantin; Petrolini, Alessandro; Picatoste Olloqui, Eduardo; Pietrzyk, Boleslaw; Pilař, Tomas; Pinci, Davide; Pistone, Alessandro; Playfer, Stephen; Plo Casasus, Maximo; Polci, Francesco; Poluektov, Anton; Polycarpo, Erica; Popov, Alexander; Popov, Dmitry; Popovici, Bogdan; Potterat, Cédric; Price, Eugenia; Prisciandaro, Jessica; Pritchard, Adrian; Prouve, Claire; Pugatch, Valery; Puig Navarro, Albert; Punzi, Giovanni; Qian, Wenbin; Rachwal, Bartolomiej; Rademacker, Jonas; Rakotomiaramanana, Barinjaka; Rama, Matteo; Rangel, Murilo; Raniuk, Iurii; Rauschmayr, Nathalie; Raven, Gerhard; Reichert, Stefanie; Reid, Matthew; dos Reis, Alberto; Ricciardi, Stefania; Richards, Sophie; Rihl, Mariana; Rinnert, Kurt; Rives Molina, Vincente; Roa Romero, Diego; Robbe, Patrick; Rodrigues, Ana Barbara; Rodrigues, Eduardo; Rodriguez Perez, Pablo; Roiser, Stefan; Romanovsky, Vladimir; Romero Vidal, Antonio; Rotondo, Marcello; Rouvinet, Julien; Ruf, Thomas; Ruffini, Fabrizio; Ruiz, Hugo; Ruiz Valls, Pablo; Sabatino, Giovanni; Saborido Silva, Juan Jose; Sagidova, Naylya; Sail, Paul; Saitta, Biagio; Salustino Guimaraes, Valdir; Sanchez Mayordomo, Carlos; Sanmartin Sedes, Brais; Santacesaria, Roberta; Santamarina Rios, Cibran; Santovetti, Emanuele; Sapunov, Matvey; Sarti, Alessio; Satriano, Celestina; Satta, Alessia; Saunders, Daniel Martin; Savrie, Mauro; Savrina, Darya; Schiller, Manuel; Schindler, Heinrich; Schlupp, Maximilian; Schmelling, Michael; Schmidt, Burkhard; Schneider, Olivier; Schopper, Andreas; Schune, Marie Helene; Schwemmer, Rainer; Sciascia, Barbara; Sciubba, Adalberto; Seco, Marcos; Semennikov, Alexander; Sepp, Indrek; Serra, Nicola; Serrano, Justine; Sestini, Lorenzo; Seyfert, Paul; Shapkin, Mikhail; Shapoval, Illya; Shcheglov, Yury; Shears, Tara; Shekhtman, Lev; Shevchenko, Vladimir; Shires, Alexander; Silva Coutinho, Rafael; Simi, Gabriele; Sirendi, Marek; Skidmore, Nicola; Skwarnicki, Tomasz; Smith, Anthony; Smith, Edmund; Smith, Eluned; Smith, Jackson; Smith, Mark; Snoek, Hella; Sokoloff, Michael; Soler, Paul; Soomro, Fatima; Souza, Daniel; Souza De Paula, Bruno; Spaan, Bernhard; Sparkes, Ailsa; Spradlin, Patrick; Stagni, Federico; Stahl, Marian; Stahl, Sascha; Steinkamp, Olaf; Stenyakin, Oleg; Stevenson, Scott; Stoica, Sabin; Stone, Sheldon; Storaci, Barbara; Stracka, Simone; Straticiuc, Mihai; Straumann, Ulrich; Stroili, Roberto; Subbiah, Vijay Kartik; Sun, Liang; Sutcliffe, William; Swientek, Krzysztof; Swientek, Stefan; Syropoulos, Vasileios; Szczekowski, Marek; Szczypka, Paul; Szilard, Daniela; Szumlak, Tomasz; T'Jampens, Stephane; Teklishyn, Maksym; Tellarini, Giulia; Teubert, Frederic; Thomas, Christopher; Thomas, Eric; van Tilburg, Jeroen; Tisserand, Vincent; Tobin, Mark; Tolk, Siim; Tomassetti, Luca; Tonelli, Diego; Topp-Joergensen, Stig; Torr, Nicholas; Tournefier, Edwige; Tourneur, Stephane; Tran, Minh Tâm; Tresch, Marco; Tsaregorodtsev, Andrei; Tsopelas, Panagiotis; Tuning, Niels; Ubeda Garcia, Mario; Ukleja, Artur; Ustyuzhanin, Andrey; Uwer, Ulrich; Vagnoni, Vincenzo; Valenti, Giovanni; Vallier, Alexis; Vazquez Gomez, Ricardo; Vazquez Regueiro, Pablo; Vázquez Sierra, Carlos; Vecchi, Stefania; Velthuis, Jaap; Veltri, Michele; Veneziano, Giovanni; Vesterinen, Mika; Viaud, Benoit; Vieira, Daniel; Vieites Diaz, Maria; Vilasis-Cardona, Xavier; Vollhardt, Achim; Volyanskyy, Dmytro; Voong, David; Vorobyev, Alexey; Vorobyev, Vitaly; Voß, Christian; Voss, Helge; de Vries, Jacco; Waldi, Roland; Wallace, Charlotte; Wallace, Ronan; Walsh, John; Wandernoth, Sebastian; Wang, Jianchun; Ward, David; Watson, Nigel; Websdale, David; Whitehead, Mark; Wicht, Jean; Wiedner, Dirk; Wilkinson, Guy; Williams, Matthew; Williams, Mike; Wilson, Fergus; Wimberley, Jack; Wishahi, Julian; Wislicki, Wojciech; Witek, Mariusz; Wormser, Guy; Wotton, Stephen; Wright, Simon; Wu, Suzhi; Wyllie, Kenneth; Xie, Yuehong; Xing, Zhou; Xu, Zhirui; Yang, Zhenwei; Yuan, Xuhao; Yushchenko, Oleg; Zangoli, Maria; Zavertyaev, Mikhail; Zhang, Liming; Zhang, Wen Chao; Zhang, Yanxi; Zhelezov, Alexey; Zhokhov, Anatoly; Zhong, Liang; Zvyagin, Alexander


    The first observation of the Cabibbo-suppressed decay $\\Lambda_b^0\\rightarrow J/\\psi p \\pi^-$ is reported using a data sample of proton-proton collisions at 7 and 8 TeV, corresponding to an integrated luminosity of 3 $\\rm fb^{-1}$. A prominent signal is observed and the branching fraction relative to the decay mode $\\Lambda_b^0\\rightarrow J/\\psi p K^-$ is determined to be $$ \\frac{{\\cal B}(\\Lambda_b^0\\rightarrow J/\\psi p \\pi^-)}{{\\cal B}(\\Lambda_b^0\\rightarrow J/\\psi p K^-)}=0.0824\\pm0.0025\\:(\\text{stat})\\pm0.0042\\:(\\text{syst}). $$ A search for direct CP violation is performed. The difference in the CP asymmetries between these two decays is found to be $$ {\\cal A}_{CP}(\\Lambda_b^0\\rightarrow J/\\psi p \\pi^-)-{\\cal A}_{CP}(\\Lambda_b^0\\rightarrow J/\\psi p K^-)=(+5.7\\pm 2.3\\:(\\text{stat})\\pm1.2\\:(\\text{syst}))\\%, $$ which is compatible with CP symmetry at the $2.2\\sigma$ level.

  19. Questioning Questions: Elementary Teachers' Adaptations of Investigation Questions Across the Inquiry Continuum (United States)

    Biggers, Mandy


    Questioning is a central practice in science classrooms. However, not every question translates into a "good" science investigation. Questions that drive science investigations can be provided by many sources including the teacher, the curriculum, or the student. The variations in the source of investigation questions were explored in this study. A dataset of 120 elementary science classroom videos and associated lesson plans from 40 elementary teachers (K-5) across 21 elementary school campuses were scored on an instrument measuring the amount of teacher-direction or student-direction of the lessons' investigation questions. Results indicated that the investigation questions were overwhelmingly teacher directed in nature, with no opportunities for students to develop their own questions for investigation. This study has implications for researchers and practitioners alike, calling attention to the teacher-directed nature of investigation questions in existing science curriculum materials, and the need for teacher training in instructional strategies to adapt their existing curriculum materials across the continuum of teacher-directed and student-directed investigation questions. Teachers need strategies for adapting the teacher-directed questions provided in their existing curriculum materials in order to allow students the opportunity to engage in this essential scientific practice.

  20. Mast cells are dispensable in a genetic mouse model of chronic dermatitis. (United States)

    Sulcova, Jitka; Meyer, Michael; Guiducci, Eva; Feyerabend, Thorsten B; Rodewald, Hans-Reimer; Werner, Sabine


    Chronic inflammatory skin diseases, such as atopic dermatitis, affect a large percentage of the population, but the role of different immune cells in the pathogenesis of these disorders is largely unknown. Recently, we found that mice lacking fibroblast growth factor receptor 1 (Fgfr1) and Fgfr2 (K5-R1/R2 mice) in the epidermis have a severe impairment in the epidermal barrier, which leads to the development of a chronic inflammatory skin disease that shares many features with human atopic dermatitis. Using Fgfr1-/Fgfr2-deficient mice, we analyzed the consequences of the loss of mast cells. Mast cells accumulated and degranulated in the skin of young Fgfr1-/Fgfr2-deficient mice, most likely as a consequence of increased expression of the mast cell chemokine Ccl2. The increase in mast cells occurred before the development of histological abnormalities, indicating a functional role of these cells in the inflammatory skin phenotype. To test this hypothesis, we mated the Fgfr1-/Fgfr2-deficient mice with mast cell-deficient CreMaster mice. Surprisingly, loss of mast cells did not or only mildly affect keratinocyte proliferation, epidermal thickness, epidermal barrier function, accumulation and activation of different immune cells, or expression of different proinflammatory cytokines in the skin. These results reveal that mast cells are dispensable for the development of chronic inflammation in response to a defect in the epidermal barrier. Copyright © 2015 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.


    Directory of Open Access Journals (Sweden)

    Surtinah Surtinah


    Full Text Available Research conducted experiments using a completely randomized design environment with three replications, and design of treatment that is the time of planting silk kale as secondary crops to the cultivation of sweet corn (K consisting of: k1 = planting at the same time; k2 = time of planting silk kale 1 week after planting sweet corn; k3 = time of planting silk kale 2 weeks after planting sweet corn; k4 = time of planting silk kale 3 weeks after planting sweet corn; K5 = single planting sweet corn planting time = k1; k6 = single silk kale planting time = k1. The results showed that silk kale can be planted together with sweet corn planting time, up to three weeks after planting sweet corn. Results of sweet corn is not influenced by the timing of planting different silk kale. Land values in the cropping system Equality sweet corn with silk kale is 1.83. Competition index between sweet corn and silk kale is 1.73.

  2. Effects of Iron-Oxide Nanoparticle Surface Chemistry on Uptake Kinetics and Cytotoxicity in CHO-K1 Cells

    Directory of Open Access Journals (Sweden)

    Camille C. Hanot


    Full Text Available Superparamagnetic iron-oxide nanoparticles (SPIONs show great promise for multiple applications in biomedicine. While a number of studies have examined their safety profile, the toxicity of these particles on reproductive organs remains uncertain. The goal of this study was to evaluate the cytotoxicity of starch-coated, aminated, and PEGylated SPIONs on a cell line derived from Chinese Hamster ovaries (CHO-K1 cells. We evaluated the effect of particle diameter (50 and 100 nm and polyethylene glycol (PEG chain length (2k, 5k and 20k Da on the cytotoxicity of SPIONs by investigating cell viability using the tetrazolium dye 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT and sulforhodamine B (SRB assays. The kinetics and extent of SPION uptake by CHO-K1 cells was also studied, as well as the resulting generation of intracellular reactive oxygen species (ROS. Cell toxicity profiles of SPIONs correlated strongly with their cellular uptake kinetics, which was strongly dependent on surface properties of the particles. PEGylation caused a decrease in both uptake and cytotoxicity compared to aminated SPIONs. Interestingly, 2k Da PEG-modifed SPIONs displayed the lowest cellular uptake and cytotoxicity among all studied particles. These results emphasize the importance of surface coatings when engineering nanoparticles for biomedical applications.

  3. Interaction of Citrinin with Human Serum Albumin

    Directory of Open Access Journals (Sweden)

    Miklós Poór


    Full Text Available Citrinin (CIT is a mycotoxin produced by several Aspergillus, Penicillium, and Monascus species. CIT occurs worldwide in different foods and drinks and causes health problems for humans and animals. Human serum albumin (HSA is the most abundant plasma protein in human circulation. Albumin forms stable complexes with many drugs and xenobiotics; therefore, HSA commonly plays important role in the pharmacokinetics or toxicokinetics of numerous compounds. However, the interaction of CIT with HSA is poorly characterized yet. In this study, the complex formation of CIT with HSA was investigated using fluorescence spectroscopy and ultrafiltration techniques. For the deeper understanding of the interaction, thermodynamic, and molecular modeling studies were performed as well. Our results suggest that CIT forms stable complex with HSA (logK ~ 5.3 and its primary binding site is located in subdomain IIA (Sudlow’s Site I. In vitro cell experiments also recommend that CIT-HSA interaction may have biological relevance. Finally, the complex formations of CIT with bovine, porcine, and rat serum albumin were investigated, in order to test the potential species differences of CIT-albumin interactions.

  4. On the magnetism of the C14 Nb0.975Fe2.025 Laves phase compound: Determination of the H-T phase diagram (United States)

    Bałanda, Maria; Dubiel, Stanisław M.


    A C14 Nb0.975Fe2.025 Laves phase compound was investigated aimed at determining the H-T magnetic phase diagram. Magnetization, M, and AC magnetic susceptibility measurement were performed. Concerning the former field-cooled and zero-field-cooled M-curves were recorded in the temperature range of 2-200 K and in applied magnetic field, H, up to 1000 Oe, isothermal M(H) curves at 2 K, 5 K, 50 K, 80 K and 110 K as well as hysteresis loops at several temperatures over the field range of ±10 kOe were measured. Regarding the AC susceptibility, χ, both real and imaginary components were registered as a function of increasing temperature in the interval of 2 K-150 K at the frequencies of the oscillating field, f, from 3 Hz up to 999 Hz. An influence of the external DC magnetic field on the temperature dependence of χ was investigated, too. The measurements clearly demonstrated that the magnetism of the studied sample is weak, itinerant and has a reentrant character. Based on the obtained results a magnetic phase diagram has been constructed in the H-T coordinates.

  5. Meta-analysis of the association of the SLC6A3 3'-UTR VNTR with cognition. (United States)

    Ettinger, Ulrich; Merten, Natascha; Kambeitz, Joseph


    The gene coding for the dopamine transporter (DAT), SLC6A3, contains a 40-base pair variable number of tandem repeats (VNTR) polymorphism (rs28363170) in its 3' untranslated region. This VNTR has been associated with attention deficit hyperactivity disorder (ADHD) and has been investigated in relation to cognition and brain function. Here, we report the results of a comprehensive meta-analysis with meta-regression examining the association of the VNTR with different domains of cognition in healthy adults. We extracted data from 28 independent studies and carried out meta-analyses for associations with working memory (k=10 samples, N=1193 subjects), inhibition (k=8 samples, N=829 subjects), executive functions including inhibition (k=10 samples, N=984 subjects), attention (k=6 samples, N=742 subjects) and declarative long-term memory (k=5 samples, N=251 subjects). None of the investigated dimensions showed significant associations with the VNTR (all p>0.26). Meta-regression including year of publication, gender, age, ethnicity and percentage of 10R-homozygotes similarly did not attain significance. We conclude that there is no evidence that rs28363170 may be a significant predictor of cognitive function in healthy adults. Copyright © 2015 Elsevier Ltd. All rights reserved.

  6. Design And Construction Of Digital Multi-Meter Using PIC Microcontroller

    Directory of Open Access Journals (Sweden)

    Khawn Nue


    Full Text Available Abstract This thesis describes the design and construction of digital multi-meter using PIC microcontroller. In this system a typical multi-meter may include features such as the ability to measure ACDC voltage DC current resistance temperature diodes frequency and connectivity. This design uses of the PIC microcontroller voltage rectifiers voltage divide potentiometer LCD and other instruments to complete the measure. When we used what we have learned of microprocessors and adjust the program to calculate and show the measures in the LCD keypad selected the modes. The software programming has been incorporated using MPLAB and PROTEUS. In this system the analogue input is taken directly to the analogue input pin of the microcontroller without any other processing. So the input range is from 0V to 5V the maximum source impedance is 2k5 for testing use a 1k pot. To improve the circuit adds an op-amp in front to present greater impedance to the circuit under test. The output impedance of the op-amp will be low which a requirement of the PIC analogue input is.

  7. Aplication Of PGR IAA And Kinetin On Rubber Clones To Accelarate Mature Tapping

    Directory of Open Access Journals (Sweden)

    Try Koryati


    Full Text Available This study aims to determine the most appropriate concentration of IAA hormones Kinetin on rubber clones to accelerate the mature tapping. Experiments have been carried out in Karang Inong Plantation PTP N I Langsa East Aceh. This study is arranged in two factors of Nested Design. Clones factor is consisted of 5 level and IAA hormon Kinetin factor has 7 levels. The results showed that the concentration of IAA hormones Kinetin is different to each clone to accelerate mature tapping. Application of IAA hormone Kinetin significantly affect the parameters of girth bark thickness number of latex vessels latex vessel diameter leaf area and the amount of chlorophyll. Application of IAA 600ppm Kinetin 60 ppm H6 show the largest increase on the girth. Clone treatment is also has significant effect on all parameters but the largest girth found in clones PB 330 and IRR 5. Combination of Clones with IAA hormone Kinetin significantly affects the bark thickness the number of latex vessels and latex vessel diameter. Application of IAA 400 ppm Kinetin 60 ppm has no significant effect on girth but able to accelerate the mature tapping particularly for clones PB 330 and IRR5 K2H4 and K5H4 as indicated by girth size namely 48.15 cm and 48.20 cm respectively at planting age 46 months.

  8. ASK1 regulates the survival of neuroblastoma cells by interacting with TLX and stabilizing HIF-1α. (United States)

    Sobhan, Praveen K; Zhai, Qiwei; Green, Lydia C; Hansford, Loen M; Funa, Keiko


    Elevated expression of TLX (also called as NR2E1) in neuroblastoma (NB) correlates with unfavorable prognosis, and TLX is required for self-renewal of NB cells. Knockdown of TLX has been shown to reduce the NB sphere-forming ability. ASK1 (MAP3K5) and TLX expression are both enhanced in SP (side population) NB and patient-derived primary NB sphere cell lines, but the majority of non-SP NB lines express lower ASK1 expression. We found that ASK1 phosphorylated and stabilized TLX, which led induction of HIF-1α, and its downstream VEGF-A in an Akt dependent manner. In depleting ASK1 upon hypoxia, TLX decreased and the apoptosis ratio of NB cells was enhanced, while low-ASK1-expressing NB cell lines were refractory in TUNEL assay by using flow cytometry. Interestingly, primary NB spheres cell lines express only high levels of active pASK1Thr-838 but the established cell lines expressed inhibitory pASK1Ser-966, and both could be targeted by ASK1 depletion. We report a novel pro-survival role of ASK1 in the tumorigenic NB cell populations, which may be applied as a therapeutic target, inducing apoptosis specifically in cancer stem cells. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

  9. Identification of nonlinear coupling in wave turbulence at the surface of water (United States)

    Campagne, Antoine; Hassaini, Roumaissa; Redor, Ivan; Aubourg, Quentin; Sommeria, Joël; Mordant, Nicolas


    The Weak Turbulence Theory is a theory, in the limit of vanishing nonlinearity, that derive analytically statistical features of wave turbulence. The stationary spectrum for the surface elevation in the case of gravity waves, is predicted to E(k) k - 5 / 2 . This spectral exponent -5/2 remains elusive in all experiments. in which the measured exponent is systematically lower than the prediction. Furthermore in the experiments the weaker the nonlinearity the further the spectral exponent is from the prediction. In order to investigate the reason for this observation we developed an experiment in the CORIOLIS facility in Grenoble. It is a 13m-diameter circular pool filled with water with a 70 cm depth. We generate wave turbulence by using two wedge wavemakers. Surface elevation measurements are performed by a stereoscopic optical technique and by capacitive probes. The nonlinear coupling at work in this system are analyzed by computing 3- and 4-wave correlations of the Fourier wave amplitudes in frequency. Theory predicts that coupling should occur through 4-wave resonant interaction. In our data, strong 3-wave correlations are observed in addition to the 4-wave correlation. Most our observations are consistent with field observation in the Black Sea (Leckler et al. 2015). This project has received funding from the European Research Council (ERC, Grant Agreement No 647018-WATU).

  10. Replication stress interferes with histone recycling and predeposition marking of new histones

    DEFF Research Database (Denmark)

    Jasencakova, Zuzana; Scharf, Annette N D; Ask, Katrine


    To restore chromatin on new DNA during replication, recycling of histones evicted ahead of the fork is combined with new histone deposition. The Asf1 histone chaperone, which buffers excess histones under stress, is a key player in this process. Yet how histones handled by human Asf1 are modified...... remains unclear. Here we identify marks on histones H3-H4 bound to Asf1 and changes induced upon replication stress. In S phase, distinct cytosolic and nuclear Asf1b complexes show ubiquitous H4K5K12diAc and heterogeneous H3 marks, including K9me1, K14ac, K18ac, and K56ac. Upon acute replication arrest......, the predeposition mark H3K9me1 and modifications typical of chromatin accumulate in Asf1 complexes. In parallel, ssDNA is generated at replication sites, consistent with evicted histones being trapped with Asf1. During recovery, histones stored with Asf1 are rapidly used as replication resumes. This shows...

  11. Analytical fits to the synchrotron functions (United States)

    Fouka, Mourad; Ouichaoui, Saad


    Accurate fitting formulae to the synchrotron function, F(x), and its complementary function, G(x), are performed and presented. The corresponding relative errors are less than 0.26% and 0.035% for F(x) and G(x), respectively. To this end we have, first, fitted the modified Bessel functions, K5/3(x) and K2/3(x). For all the fitted functions, the general fit expression is the same, and is based on the well known asymptotic forms for low and large values of x for each function. It consists of multiplying each asymptotic form by a function that tends to unity or zero for low and large values of x. Simple formulae are suggested in this paper, depending on adjustable parameters. The latter have been determined by adopting the Levenberg-Marquardt algorithm. The proposed formulae should be of great utility and simplicity for computing spectral powers and the degree of polarization for synchrotron radiation, both for laboratory and astrophysical applications.

  12. Phosphite radicals and their reactions. Examples of redox, substitution, and addition reactions

    International Nuclear Information System (INIS)

    Schaefer, K.; Asmus, K.D.


    Phosphite radicals HPO 3 - and PO 3 2 -, which exist in an acid-base equilibrium with pK = 5.75, are shown to take part in various types of reactions. In the absence of scavengers, they disappear mainly by second-order disproportionation and combination; a first-order contribution to the decay is also indicated. HPO 3 - and PO 3 2 - are good reductants toward electron acceptors such as tetranitromethane. In this reaction phosphate and C(NO 2 ) 3 - are formed. Phosphite radicals can, however, also act as good oxidants, e.g., toward thiols and thiolate ions. These reactions lead to the formation of RS. radicals which were identified either directly, as in the case of penicillamine, through the optical absorption of PenS. or more indirectly through equilibration of RS. with RS- to the optically absorbing RSSR-. disulfide radical anion. A homolytic substitution reaction (S/sub H/2) occurs in the reaction of the phosphite radicals with aliphatic disulfides, yielding RS. radicals and phosphate thioester RSPO 3 2 -. Lipoic acid, as an example of a cyclic disulfide, is reduced to the corresponding RSSR-. radical anion and also undergoes the S/sub H/2 reaction with about equal probability. An addition reaction is observed between phosphite radicals and molecular oxygen. The resulting peroxo phosphate radicals establish an acid-base equilibrium HPO 5 - . reversible PO 5 2- . + H+ with a pK = 3.4. Absolute rate constants were determined for all reactions discussed

  13. Free radical-mediated stimulation of tyrosine-specific protein kinase in rat liver plasma membrane

    International Nuclear Information System (INIS)

    Chan, T.M.; Tatoyan, A.; Cheng, E.; Shargill, N.S.; Pleta, M.


    Incorporation of 32 P from (γ- 32 P)-ATP into endogenous proteins of plasma membranes isolated from rat liver was significantly increased by several naphthoquinones including menadione. This apparent stimulation of membrane-associated protein kinase activity by these compounds was most striking (up to 6-7 fold) when the synthetic copolymers containing glutamate and tyrosine residues (4:1) was used as substrate. Since tyrosine residues are the only possible phosphate acceptor in the copolymers, the quinone-stimulated liver membrane protein kinase is most likely tyrosine specific. Although not required for protein kinase activity, dithiothreitol (DTT) was necessary for its stimulation by these quinonoid compounds. Hydrolysis of ATP was not significantly affected by quinones under the experimental conditions. Both menadione and vitamin k 5 increased phosphorylation of plasma membrane proteins of molecular weight 45 and 60 kd. The stimulatory effect of menadione on protein phosphorylation was prevented by the addition of superoxide dismutase. Dihydroxyfumerate, which spontaneously produces various radical species, and H 2 O 2 , also stimulated tyrosine-specific protein phosphorylation. DTT was also required for their full effect. It, therefore, appears that quinonone stimulation of tyrosine-specific protein phosphorylation is mediated by oxygen radicals

  14. Using insects for STEM outreach: Development and evaluation of the UA Insect Discovery Program (United States)

    Beal, Benjamin D.

    Science and technology impact most aspects of modern daily life. It is therefore important to create a scientifically literate society. Since the majority of Americans do not take college-level science courses, strong K-12 science education is essential. At the K-5 level, however, many teachers lack the time, resources and background for effective science teaching. Elementary teachers and students may benefit from scientist-led outreach programs created by Cooperative Extension or other institutions. One example is the University of Arizona Insect Discovery Program, which provides short-duration programing that uses insects to support science content learning, teach critical thinking and spark interest in science. We conducted evaluations of the Insect Discovery programming to determine whether the activities offered were accomplishing program goals. Pre-post tests, post program questionnaires for teachers, and novel assessments of children's drawings were used as assessment tools. Assessments were complicated by the short duration of the program interactions with the children as well as their limited literacy. In spite of these difficulties, results of the pre-post tests indicated a significant impact on content knowledge and critical thinking skills. Based on post-program teacher questionnaires, positive impacts on interest in science learning were noted as much as a month after the children participated in the program. New programming and resources developed to widen the potential for impact are also described.

  15. Steady-state oxygen-solubility in niobium

    International Nuclear Information System (INIS)

    Schulze, K.; Jehn, H.


    During annealing of niobium in oxygen in certain temperature and pressure ranges steady states are established between the absorption of molecular oxygen and the evaporation of volatile oxides. The oxygen concentration in the niobium-oxygen α-solid solution is a function of oxygen pressure and temperature and has been redetermined in the ranges 10 -5 - 10 -2 Pa O 2 and 2,070 - 2,470 K. It follows differing from former results the equation csub(o) = 9.1 x 10 -6 x sub(po2) x exp (502000/RT) with csub(o) in at.-ppm, sub(po2) in Pa, T in K, R = 8.31 J x mol -1 x K -1 . The existence of steady states is limited to a temperature range from 1870 to 2470 K and to oxygen concentrations below the solubility limit given by solidus and solvus lines in the T-c diagram. In the experiments high-purity niobium wires with a specific electrical ratio rho (273 K)/rho(4.2 K) > 5,000 have been gassed under isothermal-isobaric conditions until the steady state has been reached. The oxygen concentration has been determined analytically by vacuum fusion extraction with platinum-flux technique as well as by electrical residual resistivity measurements at 4.2 K. (orig.) [de

  16. Indoline Amide Glucosides from Portulaca oleracea: Isolation, Structure, and DPPH Radical Scavenging Activity. (United States)

    Jiao, Ze-Zhao; Yue, Su; Sun, Hong-Xiang; Jin, Tian-Yun; Wang, Hai-Na; Zhu, Rong-Xiu; Xiang, Lan


    A polyamide column chromatography method using an aqueous ammonia mobile phase was developed for large-scale accumulation of water-soluble indoline amide glucosides from a medicinal plant, Portulaca oleracea. Ten new [oleraceins H, I, K, L, N, O, P, Q, R, S (1-10)] and four known [oleraceins A-D (11-14)] indoline amide glucosides were further purified and structurally characterized by various chromatographic and spectroscopic methods. The DPPH radical scavenging activities of oleraceins K (5) and L (6), with EC50 values of 15.30 and 16.13 μM, respectively, were twice that of a natural antioxidant, vitamin C; the EC50 values of the 12 other indoline amides, which ranged from 29.05 to 43.52 μM, were similar to that of vitamin C. Structure-activity relationships indicated that the DPPH radical scavenging activities of these indoline amides correlate with the numbers and positions of the phenolic hydroxy groups.

  17. Electrocardiographic manifestations of hyperkalemia in hemodialysis patients

    Directory of Open Access Journals (Sweden)

    Nemati Eghlim


    Full Text Available This study was performed to evaluate whether any electrocardiogram (ECG para-meter can predict the presence of hyperkalemia in patients on maintenance hemodialysis (HD. In January 2006, we conducted a cross-sectional study of 80 stable patients with end-stage renal disease from four university-based HD units of Tehran, Iran, receiving conventional thrice-weekly HD. Pre-HD serum electrolyte values and conventional 12-lead ECG were obtained from each pa-tient. Bivariate linear regression was used for assessing relationship of the study variables with hyperkalemia (K + > 5.2 mg/dL. Multivariable logistic regression was used for evaluating inde-pendent relationship between decreased T wave duration (≤ 170 ms and other variables. Bivariate correlation analysis showed a significant inverse correlation between serum potassium concentration and T wave duration (P < 0.05. None of the patients with serum potassium of ≥ 5.6 mg/dL had T wave duration > 200 ms. Multivariate logistic analysis, after adjustment for other factors, also showed a significant relationship between decreased T wave duration (≤ 170 ms and hyper-kalemia. We conclude that although hyperkalemia does not induce the usual ECG changes in HD patients, decreased T wave duration was found to be a good indicator of this lethal condition.

  18. Fabrication and properties of multifilamentary MgB 2 wires by in-situ powder-in-tube process (United States)

    Wang, Q. Y.; Jiao, G. F.; Liu, G. Q.; Xiong, X. M.; Yan, S. C.; Zhang, P. X.; Sulpice, A.; Mossang, E.; Feng, Y.; Yan, G.


    We have fabricated the long TiC-doped MgB2 wires with 6 filaments by in-situ powder-in-tube method using Nb as the barrier and copper as the stabilizer. To improve the strength of wires, the Nb-core was used as the central filament. The transport engineering critical current density (Jce) of the samples sintered at different temperature were measured, which reaches 2.5 × 104 A/cm2 at 4.2 K, 5 T. 100 m MgB2 wires with different diameter were wound into coils and the transport critical current (Ic) of the coil were measured at 30 K in self-field. The Jce value 100 m coil achieves 1.1 × 104 A/cm2 in 1.2 mm wire. The reasons leading to the enhancement of high field Jce were discussed. The results show a good potential to fabricate high performance MgB2 wires and tapes at ambient pressure on an industrial scale.

  19. Use of gamma rays to induce mutants resistant to cocoa swollen shoot disease in Theobroma cacao L

    International Nuclear Information System (INIS)

    Adu Ampomah, Y.; Owusu, G.K.; Sackey, S.; Padi, B.; Abdul Karimu, A.


    Vegetative buds of three cocoa varieties, ‘Amelonado’ (P30), ‘Trinitario’ (K5) and ‘Upper Amazon’ (T85/799) were irradiated with 15, 20 and 25 Gy of γ-rays, respectively, and budded on to rootstocks to generate MV1 shoots. The terminal buds of the shoots were removed to induce the formation of MV2 shoots, from which MV3 shoots were similarly derived. The MV3 plants were screened for resistance to the Cocoa Swollen Shoot Virus (CSSV) by patch-graft inoculation of the root-stocks. Only a few plants from each of the three cocoa varieties were found to be symptomless after indexing. These plants were multiplied by budding to the MV4 and MV5 stages and screened at each stage for CSSV resistance by inoculation using virus-carrying mealybugs. At the MV5 stage, some plants still remained symptomless and this was confirmed with enzyme-linked immunosorbent assay (ELISA). The study reveals that γ-rays can be used to induce genetic variability for resistance to CSSV in cocoa, as well as for other traits such as chlorophyll deficiency. (author)

  20. First Observation of a Baryonic B_{s}^{0} Decay. (United States)

    Aaij, R; Adeva, B; Adinolfi, M; Ajaltouni, Z; Akar, S; Albrecht, J; Alessio, F; Alexander, M; Ali, S; Alkhazov, G; Alvarez Cartelle, P; Alves, A A; Amato, S; Amerio, S; Amhis, Y; An, L; Anderlini, L; Andreassi, G; Andreotti, M; Andrews, J E; Appleby, R B; Archilli, F; d'Argent, P; Arnau Romeu, J; Artamonov, A; Artuso, M; Aslanides, E; Auriemma, G; Baalouch, M; Babuschkin, I; Bachmann, S; Back, J J; Badalov, A; Baesso, C; Baker, S; Balagura, V; Baldini, W; Baranov, A; Barlow, R J; Barschel, C; Barsuk, S; Barter, W; Baryshnikov, F; Baszczyk, M; Batozskaya, V; Battista, V; Bay, A; Beaucourt, L; Beddow, J; Bedeschi, F; Bediaga, I; Beiter, A; Bel, L J; Bellee, V; Belloli, N; Belous, K; Belyaev, I; Ben-Haim, E; Bencivenni, G; Benson, S; Beranek, S; Berezhnoy, A; Bernet, R; Bertolin, A; Betancourt, C; Betti, F; Bettler, M-O; van Beuzekom, M; Bezshyiko, Ia; Bifani, S; Billoir, P; Birnkraut, A; Bitadze, A; Bizzeti, A; Blake, T; Blanc, F; Blouw, J; Blusk, S; Bocci, V; Boettcher, T; Bondar, A; Bondar, N; Bonivento, W; Bordyuzhin, I; Borgheresi, A; Borghi, S; Borisyak, M; Borsato, M; Bossu, F; Boubdir, M; Bowcock, T J V; Bowen, E; Bozzi, C; Braun, S; Britton, T; Brodzicka, J; Buchanan, E; Burr, C; Bursche, A; Buytaert, J; Cadeddu, S; Calabrese, R; Calvi, M; Calvo Gomez, M; Camboni, A; Campana, P; Campora Perez, D H; Capriotti, L; Carbone, A; Carboni, G; Cardinale, R; Cardini, A; Carniti, P; Carson, L; Carvalho Akiba, K; Casse, G; Cassina, L; Castillo Garcia, L; Cattaneo, M; Cavallero, G; Cenci, R; Chamont, D; Charles, M; Charpentier, Ph; Chatzikonstantinidis, G; Chefdeville, M; Chen, S; Cheung, S F; Chobanova, V; Chrzaszcz, M; Chubykin, A; Cid Vidal, X; Ciezarek, G; Clarke, P E L; Clemencic, M; Cliff, H V; Closier, J; Coco, V; Cogan, J; Cogneras, E; Cogoni, V; Cojocariu, L; Collins, P; Comerma-Montells, A; Contu, A; Cook, A; Coombs, G; Coquereau, S; Corti, G; Corvo, M; Costa Sobral, C M; Couturier, B; Cowan, G A; Craik, D C; Crocombe, A; Cruz Torres, M; Cunliffe, S; Currie, R; D'Ambrosio, C; Da Cunha Marinho, F; Dall'Occo, E; Dalseno, J; Davis, A; De Aguiar Francisco, O; De Bruyn, K; De Capua, S; De Cian, M; De Miranda, J M; De Paula, L; De Serio, M; De Simone, P; Dean, C T; Decamp, D; Deckenhoff, M; Del Buono, L; Dembinski, H-P; Demmer, M; Dendek, A; Derkach, D; Deschamps, O; Dettori, F; Dey, B; Di Canto, A; Di Nezza, P; Dijkstra, H; Dordei, F; Dorigo, M; Dosil Suárez, A; Dovbnya, A; Dreimanis, K; Dufour, L; Dujany, G; Dungs, K; Durante, P; Dzhelyadin, R; Dziewiecki, M; Dziurda, A; Dzyuba, A; Déléage, N; Easo, S; Ebert, M; Egede, U; Egorychev, V; Eidelman, S; Eisenhardt, S; Eitschberger, U; Ekelhof, R; Eklund, L; Ely, S; Esen, S; Evans, H M; Evans, T; Falabella, A; Farley, N; Farry, S; Fay, R; Fazzini, D; Ferguson, D; Fernandez, G; Fernandez Prieto, A; Ferrari, F; Ferreira Rodrigues, F; Ferro-Luzzi, M; Filippov, S; Fini, R A; Fiore, M; Fiorini, M; Firlej, M; Fitzpatrick, C; Fiutowski, T; Fleuret, F; Fohl, K; Fontana, M; Fontanelli, F; Forshaw, D C; Forty, R; Franco Lima, V; Frank, M; Frei, C; Fu, J; Funk, W; Furfaro, E; Färber, C; Gabriel, E; Gallas Torreira, A; Galli, D; Gallorini, S; Gambetta, S; Gandelman, M; Gandini, P; Gao, Y; Garcia Martin, L M; García Pardiñas, J; Garra Tico, J; Garrido, L; Garsed, P J; Gascon, D; Gaspar, C; Gavardi, L; Gazzoni, G; Gerick, D; Gersabeck, E; Gersabeck, M; Gershon, T; Ghez, Ph; Gianì, S; Gibson, V; Girard, O G; Giubega, L; Gizdov, K; Gligorov, V V; Golubkov, D; Golutvin, A; Gomes, A; Gorelov, I V; Gotti, C; Govorkova, E; Graciani Diaz, R; Granado Cardoso, L A; Graugés, E; Graverini, E; Graziani, G; Grecu, A; Greim, R; Griffith, P; Grillo, L; Gruber, L; Gruberg Cazon, B R; Grünberg, O; Gushchin, E; Guz, Yu; Gys, T; Göbel, C; Hadavizadeh, T; Hadjivasiliou, C; Haefeli, G; Haen, C; Haines, S C; Hamilton, B; Han, X; Hansmann-Menzemer, S; Harnew, N; Harnew, S T; Harrison, J; Hatch, M; He, J; Head, T; Heister, A; Hennessy, K; Henrard, P; Henry, L; van Herwijnen, E; Heß, M; Hicheur, A; Hill, D; Hombach, C; Hopchev, P H; Huard, Z-C; Hulsbergen, W; Humair, T; Hushchyn, M; Hutchcroft, D; Idzik, M; Ilten, P; Jacobsson, R; Jalocha, J; Jans, E; Jawahery, A; Jiang, F; John, M; Johnson, D; Jones, C R; Joram, C; Jost, B; Jurik, N; Kandybei, S; Karacson, M; Kariuki, J M; Karodia, S; Kecke, M; Kelsey, M; Kenzie, M; Ketel, T; Khairullin, E; Khanji, B; Khurewathanakul, C; Kirn, T; Klaver, S; Klimaszewski, K; Klimkovich, T; Koliiev, S; Kolpin, M; Komarov, I; Kopecna, R; Koppenburg, P; Kosmyntseva, A; Kotriakhova, S; Kozeiha, M; Kravchuk, L; Kreps, M; Krokovny, P; Kruse, F; Krzemien, W; Kucewicz, W; Kucharczyk, M; Kudryavtsev, V; Kuonen, A K; Kurek, K; Kvaratskheliya, T; Lacarrere, D; Lafferty, G; Lai, A; Lanfranchi, G; Langenbruch, C; Latham, T; Lazzeroni, C; Le Gac, R; van Leerdam, J; Leflat, A; Lefrançois, J; Lefèvre, R; Lemaitre, F; Lemos Cid, E; Leroy, O; Lesiak, T; Leverington, B; Li, T; Li, Y; Li, Z; Likhomanenko, T; Lindner, R; Lionetto, F; Liu, X; Loh, D; Longstaff, I; Lopes, J H; Lucchesi, D; Lucio Martinez, M; Luo, H; Lupato, A; Luppi, E; Lupton, O; Lusiani, A; Lyu, X; Machefert, F; Maciuc, F; Maddock, B; Maev, O; Maguire, K; Malde, S; Malinin, A; Maltsev, T; Manca, G; Mancinelli, G; Manning, P; Maratas, J; Marchand, J F; Marconi, U; Marin Benito, C; Marinangeli, M; Marino, P; Marks, J; Martellotti, G; Martin, M; Martinelli, M; Martinez Santos, D; Martinez Vidal, F; Martins Tostes, D; Massacrier, L M; Massafferri, A; Matev, R; Mathad, A; Mathe, Z; Matteuzzi, C; Mauri, A; Maurice, E; Maurin, B; Mazurov, A; McCann, M; McNab, A; McNulty, R; Meadows, B; Meier, F; Melnychuk, D; Merk, M; Merli, A; Michielin, E; Milanes, D A; Minard, M-N; Mitzel, D S; Mogini, A; Molina Rodriguez, J; Monroy, I A; Monteil, S; Morandin, M; Morello, M J; Morgunova, O; Moron, J; Morris, A B; Mountain, R; Muheim, F; Mulder, M; Mussini, M; Müller, D; Müller, J; Müller, K; Müller, V; Naik, P; Nakada, T; Nandakumar, R; Nandi, A; Nasteva, I; Needham, M; Neri, N; Neubert, S; Neufeld, N; Neuner, M; Nguyen, T D; Nguyen-Mau, C; Nieswand, S; Niet, R; Nikitin, N; Nikodem, T; Nogay, A; O'Hanlon, D P; Oblakowska-Mucha, A; Obraztsov, V; Ogilvy, S; Oldeman, R; Onderwater, C J G; Ossowska, A; Otalora Goicochea, J M; Owen, P; Oyanguren, A; Pais, P R; Palano, A; Palutan, M; Papanestis, A; Pappagallo, M; Pappalardo, L L; Pappenheimer, C; Parker, W; Parkes, C; Passaleva, G; Pastore, A; Patel, M; Patrignani, C; Pearce, A; Pellegrino, A; Penso, G; Pepe Altarelli, M; Perazzini, S; Perret, P; Pescatore, L; Petridis, K; Petrolini, A; Petrov, A; Petruzzo, M; Picatoste Olloqui, E; Pietrzyk, B; Pikies, M; Pinci, D; Pistone, A; Piucci, A; Placinta, V; Playfer, S; Plo Casasus, M; Poikela, T; Polci, F; Poli Lener, M; Poluektov, A; Polyakov, I; Polycarpo, E; Pomery, G J; Ponce, S; Popov, A; Popov, D; Popovici, B; Poslavskii, S; Potterat, C; Price, E; Prisciandaro, J; Prouve, C; Pugatch, V; Puig Navarro, A; Punzi, G; Qian, C; Qian, W; Quagliani, R; Rachwal, B; Rademacker, J H; Rama, M; Ramos Pernas, M; Rangel, M S; Raniuk, I; Ratnikov, F; Raven, G; Ravonel Salzgeber, M; Reboud, M; Redi, F; Reichert, S; Dos Reis, A C; Remon Alepuz, C; Renaudin, V; Ricciardi, S; Richards, S; Rihl, M; Rinnert, K; Rives Molina, V; Robbe, P; Rodrigues, A B; Rodrigues, E; Rodriguez Lopez, J A; Rodriguez Perez, P; Rogozhnikov, A; Roiser, S; Rollings, A; Romanovskiy, V; Romero Vidal, A; Ronayne, J W; Rotondo, M; Rudolph, M S; Ruf, T; Ruiz Valls, P; Saborido Silva, J J; Sadykhov, E; Sagidova, N; Saitta, B; Salustino Guimaraes, V; Sanchez Gonzalo, D; Sanchez Mayordomo, C; Sanmartin Sedes, B; Santacesaria, R; Santamarina Rios, C; Santimaria, M; Santovetti, E; Sarti, A; Satriano, C; Satta, A; Saunders, D M; Savrina, D; Schael, S; Schellenberg, M; Schiller, M; Schindler, H; Schlupp, M; Schmelling, M; Schmelzer, T; Schmidt, B; Schneider, O; Schopper, A; Schreiner, H F; Schubert, K; Schubiger, M; Schune, M-H; Schwemmer, R; Sciascia, B; Sciubba, A; Semennikov, A; Sergi, A; Serra, N; Serrano, J; Sestini, L; Seyfert, P; Shapkin, M; Shapoval, I; Shcheglov, Y; Shears, T; Shekhtman, L; Shevchenko, V; Siddi, B G; Silva Coutinho, R; Silva de Oliveira, L; Simi, G; Simone, S; Sirendi, M; Skidmore, N; Skwarnicki, T; Smith, E; Smith, I T; Smith, J; Smith, M; Soares Lavra, L; Sokoloff, M D; Soler, F J P; Souza De Paula, B; Spaan, B; Spradlin, P; Sridharan, S; Stagni, F; Stahl, M; Stahl, S; Stefko, P; Stefkova, S; Steinkamp, O; Stemmle, S; Stenyakin, O; Stevens, H; Stoica, S; Stone, S; Storaci, B; Stracka, S; Stramaglia, M E; Straticiuc, M; Straumann, U; Sun, L; Sutcliffe, W; Swientek, K; Syropoulos, V; Szczekowski, M; Szumlak, T; T'Jampens, S; Tayduganov, A; Tekampe, T; Tellarini, G; Teubert, F; Thomas, E; van Tilburg, J; Tilley, M J; Tisserand, V; Tobin, M; Tolk, S; Tomassetti, L; Tonelli, D; Topp-Joergensen, S; Toriello, F; Tourinho Jadallah Aoude, R; Tournefier, E; Tourneur, S; Trabelsi, K; Traill, M; Tran, M T; Tresch, M; Trisovic, A; Tsaregorodtsev, A; Tsopelas, P; Tully, A; Tuning, N; Ukleja, A; Ustyuzhanin, A; Uwer, U; Vacca, C; Vagner, A; Vagnoni, V; Valassi, A; Valat, S; Valenti, G; Vazquez Gomez, R; Vazquez Regueiro, P; Vecchi, S; van Veghel, M; Velthuis, J J; Veltri, M; Veneziano, G; Venkateswaran, A; Verlage, T A; Vernet, M; Vesterinen, M; Viana Barbosa, J V; Viaud, B; Vieira, D; Vieites Diaz, M; Viemann, H; Vilasis-Cardona, X; Vitti, M; Volkov, V; Vollhardt, A; Voneki, B; Vorobyev, A; Vorobyev, V; Voß, C; de Vries, J A; Vázquez Sierra, C; Waldi, R; Wallace, C; Wallace, R; Walsh, J; Wang, J; Ward, D R; Wark, H M; Watson, N K; Websdale, D; Weiden, A; Whitehead, M; Wicht, J; Wilkinson, G; Wilkinson, M; Williams, M; Williams, M P; Williams, M; Williams, T; Wilson, F F; Wimberley, J; Winn, M A; Wishahi, J; Wislicki, W; Witek, M; Wormser, G; Wotton, S A; Wraight, K; Wyllie, K; Xie, Y; Xu, Z; Yang, Z; Yang, Z; Yao, Y; Yin, H; Yu, J; Yuan, X; Yushchenko, O; Zarebski, K A; Zavertyaev, M; Zhang, L; Zhang, Y; Zhelezov, A; Zheng, Y; Zhu, X; Zhukov, V; Zonneveld, J B; Zucchelli, S


    We report the first observation of a baryonic B_{s}^{0} decay, B_{s}^{0}→pΛ[over ¯]K^{-}, using proton-proton collision data recorded by the LHCb experiment at center-of-mass energies of 7 and 8 TeV, corresponding to an integrated luminosity of 3.0  fb^{-1}. The branching fraction is measured to be B(B_{s}^{0}→pΛ[over ¯]K^{-})+B(B_{s}^{0}→p[over ¯]ΛK^{+})=[5.46±0.61±0.57±0.50(B)±0.32(f_{s}/f_{d})]×10^{-6}, where the first uncertainty is statistical and the second systematic, the third uncertainty accounts for the experimental uncertainty on the branching fraction of the B^{0}→pΛ[over ¯]π^{-} decay used for normalization, and the fourth uncertainty relates to the knowledge of the ratio of b-quark hadronization probabilities f_{s}/f_{d}.

  1. Development of a thinned back-illuminated CMOS active pixel sensor for extreme ultraviolet spectroscopy and imaging in space science

    International Nuclear Information System (INIS)

    Waltham, N.R.; Prydderch, M.; Mapson-Menard, H.; Pool, P.; Harris, A.


    We describe our programme to develop a large-format, science-grade, monolithic CMOS active pixel sensor for future space science missions, and in particular an extreme ultraviolet (EUV) spectrograph for solar physics studies on ESA's Solar Orbiter. Our route to EUV sensitivity relies on adapting the back-thinning and rear-illumination techniques first developed for CCD sensors. Our first large-format sensor consists of 4kx3k 5 μm pixels fabricated on a 0.25 μm CMOS imager process. Wafer samples of these sensors have been thinned by e2v technologies with the aim of obtaining good sensitivity at EUV wavelengths. We present results from both front- and back-illuminated versions of this sensor. We also present our plans to develop a new sensor of 2kx2k 10 μm pixels, which will be fabricated on a 0.35 μm CMOS process. In progress towards this goal, we have designed a test-structure consisting of six arrays of 512x512 10 μm pixels. Each of the arrays has been given a different pixel design to allow verification of our models, and our progress towards optimizing a design for minimal system readout noise and maximum dynamic range. These sensors will also be back-thinned for characterization at EUV wavelengths

  2. Complete Structure of an Epithelial Keratin Dimer: Implications for Intermediate Filament Assembly.

    Directory of Open Access Journals (Sweden)

    David J Bray

    Full Text Available Keratins are cytoskeletal proteins that hierarchically arrange into filaments, starting with the dimer sub-unit. They are integral to the structural support of cells, in skin, hair and nails. In skin, keratin is thought to play a critical role in conferring the barrier properties and elasticity of skin. In general, the keratin dimer is broadly described by a tri-domain structure: a head, a central rod and a tail. As yet, no atomistic-scale picture of the entire dimer structure exists; this information is pivotal for establishing molecular-level connections between structure and function in intermediate filament proteins. The roles of the head and tail domains in facilitating keratin filament assembly and function remain as open questions. To address these, we report results of molecular dynamics simulations of the entire epithelial human K1/K10 keratin dimer. Our findings comprise: (1 the first three-dimensional structural models of the complete dimer unit, comprising of the head, rod and tail domains; (2 new insights into the chirality of the rod-domain twist gained from analysis of the full domain structure; (3 evidence for tri-subdomain partitioning in the head and tail domains; and, (4 identification of the residue characteristics that mediate non-covalent contact between the chains in the dimer. Our findings are immediately applicable to other epithelial keratins, such as K8/K18 and K5/K14, and to intermediate filament proteins in general.

  3. A comparison of model-scale experimental measurements and computational predictions for a large transom-stern wave (United States)

    O'Shea, Thomas T.; Beale, Kristy L. C.; Brucker, Kyle A.; Wyatt, Donald C.; Drazen, David; Fullerton, Anne M.; Fu, Tom C.; Dommermuth, Douglas G.


    Numerical Flow Analysis (NFA) predictions of the flow around a transom-stern hull form are compared to laboratory measurements collected at NSWCCD. The simulations are two-phase, three-dimensional, and unsteady. Each required 1.15 billion grid cells and 200,000 CPU hours to accurately resolve the unsteady flow and obtain a sufficient statistical ensemble size. Two speeds, 7 and 8 knots, are compared. The 7 knots (Fr=Uo /√gLo=0.38) case is a partially wetted transom condition and the 8 knots (Fr=0.43) case is a dry transom condition. The results of a detailed comparison of the mean free surface elevation, surface roughness (RMS), and spectra of the breaking stern-waves, measured by Light Detection And Ranging (LiDAR) and Quantitative Visualization (QViz) sensors, are presented. All of the comparisons showed excellent agreement. The concept of height-function processing is introduced, and the application of this type of processing to the simulation data shows a k-5/3 power law behavior for both the 7 and 8 knot cases. The simulations also showed that a multiphase shear layer forms in the rooster-tail region and that its thickness depends on the Froude number.

  4. Spectroscopic studies of Eu(III) Keggin's and Dawson's polyoxotungstates substituted by acetato and oxalato ligands

    International Nuclear Information System (INIS)

    But, Slawomir; Lis, Stefan


    Eu(III) Keggin's and Dawson's type of polyoxometalates (POM) complexes were synthesized and spectroscopically characterized. This work presents results obtained for chosen hybrids of acetato K 12 [{Eu(SiMo x W 11-x O 39 )(H 2 O)} 2 (CH 3 COO) 2 ].nH 2 O, K 16 [{Eu(CH 3 COO)(H 2 O) 2 (P 2 W 17 O 61 )} 2 ].nH 2 O and oxalato (NH 4 ) 29 K 5 [{Eu(P 2 W 17 O 61 )} 4 (C 2 O 4 ) 3 (H 2 O) 4 ].nH 2 O, where x = 0, 1. The solid state compositions of the hybrids were characterized by using elemental and ICP-AES analysis, derivatography and FTIR spectroscopy. Luminescence characteristics (intensity, quantum yields, luminescence lifetimes and excitation spectra in the range of the 5 D 0 7 F 0 transition) of the synthesized Eu(III) acetato and oxalato complexes were determined in the solid phase and in solution, and they were compared to their parent Eu(III):POM complexes of 1:1 stoichiometry (ML)

  5. The Application Of Open-Source And Free Photogrammetric Software For The Purposes Of Cultural Heritage Documentation

    Directory of Open Access Journals (Sweden)

    Bartoš Karol


    Full Text Available The documentation of cultural heritage is an essential part of appropriate care of historical monuments, representing a part of our history. At present, it represents the current issue, for which considerable funds are being spent, as well as for the documentation of immovable historical monuments in a form of castle ruins, among the others. Non-contact surveying technologies - terrestrial laser scanning and digital photogrammetry belong to the most commonly used technologies, by which suitable documentation can be obtained, however their use may be very costly. In recent years, various types of software products and web services based on the SfM (or MVS method and developed as open-source software, or as a freely available and free service, relying on the basic principles of photogrammetry and computer vision, have started to get into the spotlight. By using the services and software, acquired digital images of a given object can be processed into a point cloud, serving directly as a final output or as a basis for further processing. The aim of this paper, based on images of various objects of the Slanec castle ruins obtained by the DSLR Pentax K5, is to assess the suitability of different types of open-source and free software and free web services and their reliability in terms of surface reconstruction and photo-texture quality for the purposes of castle ruins documentation.

  6. Turbulence in nearly incompressible fluids: density spectrum, flows, correlations and implication to the interstellar medium

    Directory of Open Access Journals (Sweden)

    S. Dastgeer


    Full Text Available Interstellar scintillation and angular radio wave broadening measurements show that interstellar and solar wind (electron density fluctuations exhibit a Kolmogorov-like k-5/3 power spectrum extending over many decades in wavenumber space. The ubiquity of the Kolmogorov-like interstellar medium (ISM density spectrum led to an explanation based on coupling incompressible magnetohydrodynamic (MHD fluctuations to density fluctuations through a 'pseudosound' relation within the context of 'nearly incompressible' (NI hydrodynamics (HD and MHD models. The NI theory provides a fundamentally different explanation for the observed ISM density spectrum in that the density fluctuations can be a consequence of passive scalar convection due to background incompressible fluctuations. The theory further predicts generation of long-scale structures and various correlations between the density, temperature and the (magneto acoustic as well as convective pressure fluctuations in the compressible ISM fluids in different thermal regimes that are determined purely by the thermal fluctuation level. In this paper, we present the results of our two dimensional nonlinear fluid simulations, exploring various nonlinear aspects that lead to inertial range ISM turbulence within the context of a NI hydrodymanics model. In qualitative agreement with the NI predictions and the in-situ observations, we find that i the density fluctuations exhibit a Kolmogorov-like spectrum via a passive convection in the field of the background incompressible fluctuations, ii the compressible ISM fluctuations form long scale flows and structures, and iii the density and the temperature fluctuations are anti-correlated.

  7. Vascular flora of Kenya, based on the Flora of Tropical East Africa

    Directory of Open Access Journals (Sweden)

    Yadong Zhou


    Full Text Available Kenya, an African country with major higher plant diversity, has a corresponding diversity of plant associations, because of the wide geographic distribution, diverse climatic conditions and soil types. In this article, all vascular plants of Kenya were counted based on the completed "Flora of Tropical East Africa (FTEA", and all families and genera were revised using recent molecular systematics research, forming a "Synoptic List of Families and Genera of Kenyan Vascular Plants (SLFGKVP". In total, there are 225 families, 1538 genera and 6293 indigenous species and and 62 families, 302 genera and 588 exotic species in Kenya. The Fabaceae with 98 genera and 576 Species is the largest family. Two of the seven plant distribution regions of Kenya, K4 and K7 are the most species-richest areas with regard to both total and endemic species, with 3375 and 3191 total species and 174 and 185 endemic species in K4 and K7 respectively. While, K3 and K5 have the highest density of both total and endemic species. K1 has the lowest density of total species, and K2 has the lowest density of endemic species.


    Energy Technology Data Exchange (ETDEWEB)

    Mann, Andrew W.; Hilton, Eric J. [Institute for Astronomy, University of Hawaii, Honolulu, HI 96822 (United States); Gaidos, Eric [Department of Geology and Geophysics, University of Hawaii, Honolulu, HI 96822 (United States); Lepine, Sebastien, E-mail: [Department of Astrophysics, American Museum of Natural History, New York, NY 10024 (United States)


    We estimate the stellar parameters of late K- and early M-type Kepler target stars. We obtain medium-resolution visible spectra of 382 stars with K{sub P} - J > 2 ({approx_equal}K5 and later spectral type). We determine luminosity class by comparing the strength of gravity-sensitive indices (CaH, K I, Ca II, and Na I) to their strength in a sample of stars of known luminosity class. We find that giants constitute 96% {+-} 1% of the bright (K{sub P} < 14) Kepler target stars, and 7% {+-} 3% of dim (K{sub P} > 14) stars, significantly higher than fractions based on the stellar parameters quoted in the Kepler Input Catalog (KIC). The KIC effective temperatures are systematically (110{sup +15}{sub -35} K) higher than temperatures we determine from fitting our spectra to PHOENIX stellar models. Through Monte Carlo simulations of the Kepler exoplanet candidate population, we find a planet occurrence of 0.36 {+-} 0.08 when giant stars are properly removed, somewhat higher than when a KIC log g > 4 criterion is used (0.27 {+-} 0.05). Last, we show that there is no significant difference in g - r color (a probe of metallicity) between late-type Kepler stars with transiting Earth-to-Neptune-size exoplanet candidates and dwarf stars with no detected transits. We show that a previous claimed offset between these two populations is most likely an artifact of including a large number of misidentified giants.

  9. Locus of control and peer relationships among Caucasian, Hispanic, Asian, and African American adolescents. (United States)

    Kang, Hannah Soo; Chang, Kyle Edward; Chen, Chuansheng; Greenberger, Ellen


    Past research has shown that locus of control plays an important role in a wide range of behaviors, such as academic achievement and positive social behaviors. However, little is known about whether locus of control plays the same role in minority adolescents' peer relationships. The current study examined ethnic differences in the associations between locus of control and peer relationships in early adolescence using samples from the Early Childhood Longitudinal Study (ECLS-K: 5,612 Caucasian, 1,562 Hispanic, 507 Asian, and 908 African-American adolescents) and the National Education Longitudinal Study (NELS: 8,484 Caucasian, 1,604 Hispanic, and 860 Asian, and 1,228 African American adolescents). Gender was approximately evenly split in both samples. The results from the two datasets were highly consistent. Significant interactions between ethnicity and locus of control indicated that having a more internal locus of control was particularly important for Caucasian students' peer relationships (ECLS-K) and social status (NELS), but less so for Asian, Hispanic, and African American students. Our findings suggest that the role of locus of control in peer relationship is contingent upon culture.

  10. Identifikasi Kualitas Beras dengan Citra Digital

    Directory of Open Access Journals (Sweden)

    Arissa Aprilia Nurcahyani


    Full Text Available Beras merupakan makanan pokok yang paling banyak di konsumsi oleh masyarakat Indonesia. Namun, harga beras di pasaran justru semakin melonjak, sehingga banyak beredar beras yang memiliki kualitas kurang baik. Oleh karena itu perlu adanya standar kualitas mutu dari pihak gudang beras saat mendistribusikan beras ke pasaran. Standar pengujian kualitas dari pihak Bulog terdapat dua tahap, yaitu uji laboratorium dan uji visual. Namun, pengujian secara visual selama ini masih dilakukan secara manual sehingga masih sering terjadi kesalahan karena terbatasnya penglihatan manusia dan subjektivitas penguji. Oleh karena itu, sistem pengujian secara visual dengan citra digital dapat menjadi solusi yang efektif untuk permasalahan tersebut. Proses pengujian dapat dilihat dari nilai putih, nilai bersih, dan nilai utuh beras yang diakuisisi melalui pengolahan citra digital. Proses akuisisi nilai bersih dan putih dilakukan dengan menganalisis nilai HSV (Hue, Saturation, Value, sedangkan nilai utuh dilakukan dengan menganalisis luas region area objek. Sebelumnya, dilakukan training terhadap 30 data untuk mendapatkan decision tree dengan model ID3 (Iterative Dichotomiser Tree. Data yang telah diakuisisi kemudian diklasifikasi ke dalam 3 kelas yaitu baik, kurang dan buruk dengan menggunankan aturan dari decision tree yang dihasilkan pada proses training. Hasil pengujian dengan metode k-fold cross validation dengan k=5 didapatkan akurasi sebesar 96.67%.

  11. Fractal dimension of turbulent black holes (United States)

    Westernacher-Schneider, John Ryan


    We present measurements of the fractal dimension of a turbulent asymptotically anti-de Sitter black brane reconstructed from simulated boundary fluid data at the perfect fluid order using the fluid-gravity duality. We argue that the boundary fluid energy spectrum scaling as E (k )˜k-2 is a more natural setting for the fluid-gravity duality than the Kraichnan-Kolmogorov scaling of E (k )˜k-5 /3, but we obtain fractal dimensions D for spatial sections of the horizon H ∩Σ in both cases: D =2.584 (1 ) and D =2.645 (4 ), respectively. These results are consistent with the upper bound of D =3 , thereby resolving the tension with the recent claim in Adams et al. [Phys. Rev. Lett. 112, 151602 (2014), 10.1103/PhysRevLett.112.151602] that D =3 +1 /3 . We offer a critical examination of the calculation which led to their result, and show that their proposed definition of the fractal dimension performs poorly as a fractal dimension estimator on one-dimensional curves with known fractal dimension. Finally, we describe how to define and in principle calculate the fractal dimension of spatial sections of the horizon H ∩Σ in a covariant manner, and we speculate on assigning a "bootstrapped" value of fractal dimension to the entire horizon H when it is in a statistically quasisteady turbulent state.

  12. Effect of formulation variables on the physical properties and stability of Dead Sea mud masks. (United States)

    Shahin, Sawsan; Hamed, Saja; Alkhatib, Hatim S


    The physical stability of Dead Sea mud mask formulations under different conditions and their rheological properties were evaluated as a function of the type and level of thickeners, level of the humectant, incorporation of ethanol, and mode of mud treatment. Formulations were evaluated in terms of visual appearance, pH, moisture content, spreadability, extrudability, separation, rate of drying at 32 degrees C, and rheological properties. Prepared mud formulations and over-the-shelf products showed viscoplastic shear thinning behavior; satisfactory rheological behavior was observed with formulations containing a total concentration of thickeners less than 10% (w/w). Casson and Herschel-Bulkley models were found the most suitable to describe the rheological data of the prepared formulations. Thickener incorporation decreased phase separation and improved formulation stability. Bentonite incorporation in the mud prevented color changes during stability studies while glycerin improved spreadability. Addition of 5% (w/w) ethanol improved mud extrudability, slightly increased percent separation, accelerated drying at 32 degrees C, and decreased viscosity and yield stress values. Different mud treatment techniques did not cause a clear behavioral change in the final mud preparation. B10G and K5B5G were labeled as "best formulas" based on having satisfactory physical and aesthetic criteria investigated in this study, while other formulations failed in one or more of the tests we have performed.

  13. Revamping of entisol soil physical characteristics with compost treatment (United States)

    Sumono; Loka, S. P.; Nasution, D. L. S.


    Physical characteristic of Entisol soil is an important factor for the growth of plant. The aim of this research was to know the effect of compost application on physical characteristics of Entisol soil. The research method used was experimental method with 6 (six) treatments and 3 replications of which K1 = 10 kg Entisol soil without compost, K2 = 9 Kg Entisol soil with 1 kg compost, K3 = 8 kg Entisol soil with 2 kg compost, K4 = 7 kg Entisol soilwith3 kg compost, K5 = 6 kg Entisol soil with 4 kg compost and K6 = 5 kg Entisol soil with 5 kg compost. The observed parameters were soil texture, soil organic matter, soil thickness, porosity, soil pore size, soil permeability and water availability. The results showed that the Entisol soil texture was loamy sand texture, the value of soil organic matter ranged from 0.74% to 4.69%, soil thickness ranged from 13.83 to 20.16 cm, porosity ranged from16% to 37%, soil pore size ranged from 2.859 to 5.493 µm, permeability ranged from 1.24 to 5.64 cm/hour and water availability ranged from 6.67% to 9.12% by each treatment.

  14. Variability of silver fir (Abies alba Mill. cones – variability of cone parameters

    Directory of Open Access Journals (Sweden)

    Aniszewska Monika


    Full Text Available This study aimed at determining the shape of closed silver fir cones from the Jawor Forest District (Wroclaw, based purely on measurements of their length and thickness. Using these two parameters, the most accurate estimations were achieved with a fourth-degree polynomial fitting function. We then calculated the cones’ surface area and volume in three different ways: 1 Using the fourth-degree polynomial shape estimation, 2 Introducing indicators of compliance (k1, k2, k3 to calculate the volume and then comparing it to its actual value as measured in a pitcher filled with water, 3 Comparing the surface area of the cones as calculated with the polynomial function to the value obtained from ratios of indicators of compliance (ratios k4 and k5. We found that the calculated surface area and volume were substantially higher than the corresponding measured values. Test values of cone volume and surface area as calculated by our model were 8% and 5% lower, respectively, compared to direct measurements. We also determined the fir cones apparent density to be 0.8 g·cm-3on average. The gathered data on cone surface area, volume and bulk density is a valuable tool for optimizing the thermal peeling process in mill cabinets to acquire high quality seeds.

  15. H + CH{sub 2}CO {yields} CH{sub 3} + CO at high temperature : a high pressure chemical activation reaction with positive barrier.

    Energy Technology Data Exchange (ETDEWEB)

    Hranisavljevic, J.; Kumaran, S. S.; Michael, J. V.


    The Laser Photolysis-Shock Tube (LP-ST) technique coupled with H-atom atomic resonance absorption spectrometry (ARAS) has been used to study reaction, H + CH{sub 2}CO {r_arrow} CH{sub 3} + CO, over the temperature range, 863-1400 K. The results can be represented by the Arrhenius expression, k = (4.85 {+-} 0.70) x 10{sup {minus}11} exp({minus}2328 {+-} 155 K/T) cm{sup 3} molecule{sup {minus}1} s{sup {minus}1}. The present data have been combined with the earlier low temperature flash photolysis-resonance fluorescence measurements to yield a joint three parameter expression, k = 5.44 x 10{sup {minus}14} T{sup 0.8513} exp({minus}1429 K/T) cm{sup 3} molecule{sup {minus}1} s{sup {minus}1}. This is a chemical activation process that proceeds through vibrationally excited acetyl radicals. However, due to the presence of a low lying forward dissociation channel to CH{sub 3} + CO, the present results refer to the high pressure limiting rate constants. Hence, transition state theory with Eckart tunneling is used to explain the data.

  16. Effects of the learning cycle upon student and classroom teacher performance (United States)

    Marek, Edmund A.; Methven, Suzanne B.

    A great deal of research has been done regarding science in-service education and the conclusion can be drawn that positive results were generally achieved in workshop-participant attitude and implementation of instructional approaches into the classroom. One of the most important effects of an in-service workshop is upon the students of the teachers participating in an in-service program, but rarely, if ever, is this parameter assessed in the in-service evaluation design. This study investigated the relationships among (1) teacher's attitudes and implementation of in-service workshop developed science materials (learning cycles) and (2) elementary school student's conservation reasoning and language used to describe properties of objects. Data were gathered from over 100 students from grades K-5 and 16 teachers who had participated in an in-service program. A representative comparison group of students and teachers was selected which generally matched the teachers participating in the in-service workshop except for one variable--the comparison group taught science traditionally, that is, by exposition. Data from the research indicated that the teachers involved in the science in-service workshop implemented the workshop-developed learning cycles into their science classes. Significantly greater gains in conservation reasoning and language usage occurred with the students of the teachers participating in the science in-service workshop as compared to students in the exposition classrooms.

  17. Ionothermal Synthesis and Magnetic Studies of Novel Two-Dimensional Metal-Formate Frameworks

    International Nuclear Information System (INIS)

    Calderone, P.; Feygenson, M.; Forster, P.M.; Borkowski, L.A.; Teat, S.J.; Aronson, M.C.; Parise, J.B.


    Five novel two-dimensional frameworks containing formate-bridged metal-centered octahedra are synthesized ionothermally from two ionic liquids previously unused as solvents in hybrid synthesis, 2-hydroxyethylammonium (HEA) formate, and 1-hydroxy-3-proplyammonium (HPA) formate. Templating effects of the cation from each ionic liquid drive the formation of different structures. [NH 3 C 2 H 4 OH] 2 [M(CHO 2 ) 4 ] (1: M = Co, 2: M = Ni) exhibit the same stoichiometry and connectivity as their manganese analogue (3: M = Mn), but the manganese form exhibits a different topology from 1 and 2. [NH 3 C 3 H6OH][M(CHO 2 ) 3 (H 2 O)] (4: M = Co, 5: M = Mn) were synthesized using the HPA formate ionic liquid with a metal-formate connectivity related to those of 1-3. Canted antiferromagnetic ordering occurs at low temperatures (1: T N = 7.0 K, 2: T N = 4.6 K, 3: T N = 8.0 K, 4: T N = 7.0 K, 5: T N = 9.2 K), similar to the magnetic properties previously reported for other metal-formate hybrid materials.

  18. Creating a peaceful school learning environment: the impact of an antibullying program on educational attainment in elementary schools. (United States)

    Fonagy, Peter; Twemlow, Stuart W; Vernberg, Eric; Sacco, Frank C; Little, Todd D


    The impact of a bullying and violence prevention program on education attainment was studied in five elementary schools (K-5), over a 5-year period. A multiple baseline design was used and academic attainment test scores of 1,106 students were monitored before and after the introduction of the program across the school district. This sample was contrasted with an equivalent control sample of 1,100 students from the school district who attended schools that did not join the program. Program participation was associated with pronounced improvements in the students' achievement test scores. Notable reductions in the scores of those students who left schools with active programs were also observed. This simple, low-cost anti-violence intervention, involves all those who work in schools, not just students. It appears to significantly benefit educational performance of children in the participating elementary schools. The program focuses attention on the interaction between the bully, victim and audience of bystanders who are seen as pivotal in either promoting or ameliorating violence. Buy in to the philosophy by teachers & administration is high, because the format allows each school to create materials with its own personal stamp, and since there is no classroom curriculum add on, the burden to teachers is vastly reduced. Psychiatrists who work with schools could easily assist a school to put the program in place as part of their consultation work.

  19. Neutron irradiation effect on the strength of jointed Ti-6Al-4V alloy

    International Nuclear Information System (INIS)

    Ishiyama, Shintaro; Miya, Naoyuki


    In order to investigate applicability of Ti alloy to large scaled structural material for fusion reactors, irradiation effect on the mechanical properties of Ti-6Al-4V alloy and its TIG welded material was investigated after neutron irradiation (temperature: 746-788K, fluence: 2.8 x 10 23 n/m 2 (>0.18 MeV). The following results were obtained. (1) Irradiated Ti alloy shows about 20-30% increase of its tensile strength and large degradation of fracture elongation, comparing with those of unirradiated Ti alloy. (2) TIG welded material behaves as Ti alloy in its tensile test, however, shows 30% increase of area reduction in 373-473K, whereas 1/2 degradation of area reduction over 600K. (3) Irradiated TIG welded material behaves heavier embrittlement than that of irradiated Ti alloy. (4) Charpy impact properties of un- and irradiated Ti alloys shift to ductile from brittle fracture and transition temperature shift, ΔT was estimated as about 100K. (5) Remarkable increase of hardness was found, especially in HAZ of TIG welded material after irradiation. (author)

  20. Chemical composition of wet precipitation in Ibadan, Nigeria

    Directory of Open Access Journals (Sweden)

    P.C. Onianwa


    Full Text Available Rainwater samples were collected for four two-weekly periods at nine sampling points in the city of Ibadan, Nigeria, during May-July, 1999, and analysed for pH, sulphate, nitrate, chloride, phosphate, sodium, potassium, calcium, and magnesium. The rainwater was predominantly neutral with pH values ranging between 5.8 and 7.0, and averaging 6.6 plus or minus 0.3. Average concentrations (μeq/L for all the sampling points and sampling periods were: SO42- (46 plus or minus 22, NO3- (1.6 plus or minus 0.3, Cl- (11 plus or minus 13, PO43- (3.2 plus or minus 1.8, Na (11.7 plus or minus 7.9, K (5.6 plus or minus 4.4, Ca (30 plus or minus 26, and Mg (1.2 plus or minus 8.8. Concentrations of the various ions in the rainwater decreased slightly with the progress of the sampling period. The deposition flux (kg km-2 month-1 was estimated as: SO42- (390, NO3- (16.8, Cl- (67.6, PO43- (17.5, Na (46.6, K (37.9, Ca (104, and Mg (21.5.

  1. Measurement of hydroxyl radical production in ultrasonic aqueous solutions by a novel chemiluminescence method. (United States)

    Hu, Yufei; Zhang, Zhujun; Yang, Chunyan


    Measurement methods for ultrasonic fields are important for reasons of safety. The investigation of an ultrasonic field can be performed by detecting the yield of hydroxyl radicals resulting from ultrasonic cavitations. In this paper, a novel method is introduced for detecting hydroxyl radicals by a chemiluminescence (CL) reaction of luminol-hydrogen peroxide (H2O2)-K5[Cu(HIO6)2](DPC). The yield of hydroxyl radicals is calculated directly by the relative CL intensity according to the corresponding concentration of H2O2. This proposed CL method makes it possible to perform an in-line and real-time assay of hydroxyl radicals in an ultrasonic aqueous solution. With flow injection (FI) technology, this novel CL reaction is sensitive enough to detect ultra trace amounts of H2O2 with a limit of detection (3sigma) of 4.1 x 10(-11) mol L(-1). The influences of ultrasonic output power and ultrasonic treatment time on the yield of hydroxyl radicals by an ultrasound generator were also studied. The results indicate that the amount of hydroxyl radicals increases with the increase of ultrasonic output power (< or = 15 W mL(-1)). There is a linear relationship between the time of ultrasonic treatment and the yield of H2O2. The ultrasonic field of an ultrasonic cleaning baths has been measured by calculating the yield of hydroxyl radicals.

  2. A pulse radiolysis study of the OH radical induced autoxidation of methanesulfinic acid

    DEFF Research Database (Denmark)

    Sehested, K.; Holcman, J.


    Methanesulfinic acid, CH3SO2H, reacts with OH radicals at pH 7 forming CH3SO2 radicals with a rate constant k = (6.0 +/- 1.0) x 10(9) M(-1) s(-1). The CH3SO2 radical absorbs at 325 nm with an extinction coefficient of 900 +/- 100 M(-1) cm(-1) and disappears in a second order self-reaction with k...... takes place. During the course of the chain oxidation a peroxyacid, presumably methaneperoxymonosulfonic acid, is formed and accumulated. This acid absorbs in the UV and eventually decays by reaction with excess methanesulfinic acid k = 5 x 10(3) M(-1) s(-1). The final product of the chain autoxidation...... = (1.0 +/- 0.2) x 10(9) M(-1) s(-1). This radical reacts with oxygen, k = (1.2 +/- 0.3) x 10(9) M(-1) s(-1), forming a peroxy radical which absorbs in the UV below 300 nm. The peroxy radical reacts in turn with methanesulfinic acid reforming the CH3SO2 radical whereby a chain oxidation of sulfinic acid...

  3. Production and qualification of 40 km of Al-stabilized NbTi cable for the ATLAS experiment at CERN

    CERN Document Server

    Baccaglioni, G; Cartegni, G C; Horváth, I L; Neuenschwander, J; Pedrini, D; Rossi, L; Volpini, G


    The production of the conductor for the superconducting toroids of the ATLAS experiment at LHC (CERN) is now in progress. The toroid system, composed of one barrel toroid (BT) and two end cap toroids (ECTs), exploits aluminum-clad Rutherford-type NbTi conductors of large size (57 * 12 mm for BT, 42 * 12 mm for ECTs) and high critical current (Ic) (58 kA for BT and 60 kA for ECTs @ 4.2 K, 5 T). Some 55 km of conductor are required for the BT and 26 km for the ECTs, respectively. An Italian-Swiss (ETH Zurich and INFN) consortium is in charge of the delivery of half of the whole amount. This paper describes the results of this production with particular emphasis to the quality control system developed to monitor the production with both on-line controls and the post-production quality assessment protocols. The main result is the confirmation that the technologies selected and the whole process are reliable and reproducible over large production quantities. The overall degradation due to the cabling and the co-ex...

  4. Structure-based nuclear import mechanism of histones H3 and H4 mediated by Kap123

    Energy Technology Data Exchange (ETDEWEB)

    An, Sojin [Department of Biological Chemistry, University of Michigan Medical School, Michigan, United States; Yoon, Jungmin [Structural Biology Laboratory of Epigenetics, Department of Biological Sciences, Graduate school of Nanoscience and Technology (World Class University), KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, South Korea; Kim, Hanseong [Department of Biological Chemistry, University of Michigan Medical School, Michigan, United States; Song, Ji-Joon [Structural Biology Laboratory of Epigenetics, Department of Biological Sciences, Graduate school of Nanoscience and Technology (World Class University), KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, South Korea; Cho, Uhn-soo [Department of Biological Chemistry, University of Michigan Medical School, Michigan, United States


    Kap123, a major karyopherin protein of budding yeast, recognizes the nuclear localization signals (NLSs) of cytoplasmic histones H3 and H4 and translocates them into the nucleus during DNA replication. Mechanistic questions include H3- and H4-NLS redundancy toward Kap123 and the role of the conserved diacetylation of cytoplasmic H4 (K5ac and K12ac) in Kap123-mediated histone nuclear translocation. Here, we report crystal structures of full-length Kluyveromyces lactis Kap123 alone and in complex with H3- and H4-NLSs. Structures reveal the unique feature of Kap123 that possesses two discrete lysine-binding pockets for NLS recognition. Structural comparison illustrates that H3- and H4-NLSs share at least one of two lysine-binding pockets, suggesting that H3- and H4-NLSs are mutually exclusive. Additionally, acetylation of key lysine residues at NLS, particularly H4-NLS diacetylation, weakens the interaction with Kap123. These data support that cytoplasmic histone H4 diacetylation weakens the Kap123-H4-NLS interaction thereby facilitating histone Kap123-H3-dependent H3:H4/Asf1 complex nuclear translocation.

  5. ToO Galactic Nova -- Michelle ``Quick Response'' (United States)

    Helton, L. Andrew; Woodward, Chick; Evans, Nye; Geballe, Tom; Spitzer Nova Team


    Stars are the engines of energy production and chemical evolution in our Universe, depositing radiative and mechanical energy into their environments and enriching the ambient ISM with elements synthesized in their interiors and dust grains condensed in their atmospheres. Classical novae (CN) contribute to this cycle of chemical enrichment through explosive nucleosynthesis and the violent ejection of material dredged from the white dwarf progenitor and mixed with the accreted surface layers. We propose to obtain mid-IR spectra of a new galactic CN in outburst to investigate aspects of the CN phenomenon including the in situ formation and mineralogy of nova dust and the elemental abundances resulting from thermonuclear runaway. Synoptic, high S/N Michelle spectra permit: 1) determination of the grain size distribution and mineral composition of nova dust; 2) estimation of chemical abundances of nova ejecta from coronal and other emission line spectroscopy; and 3) measurement of the density and masses of the ejecta. This Gemini `Target of Opportunity' initiative (trigger K=5- 8 mag, assuming adequate PWFS guide stars exist) complements our extensive Spitzer, Chandra, Swift, XMM-Newton CN DDT/ToO programs.

  6. Synoptic Mid-IR Spectra ToO Novae (United States)

    Helton, L. Andrew; Woodward, Chick; Evans, Nye; Geballe, Tom; Spitzer Nova Team


    Stars are the engines of energy production and chemical evolution in our Universe, depositing radiative and mechanical energy into their environments and enriching the ambient ISM with elements synthesized in their interiors and dust grains condensed in their atmospheres. Classical novae (CN) contribute to this cycle of chemical enrichment through explosive nucleosynthesis and the violent ejection of material dredged from the white dwarf progenitor and mixed with the accreted surface layers. We propose to obtain mid-IR spectra of a new galactic CN in outburst to investigate aspects of the CN phenomenon including the in situ formation and mineralogy of nova dust and the elemental abundances resulting from thermonuclear runaway. Synoptic, high S/N Michelle spectra permit: 1) determination of the grain size distribution and mineral composition of nova dust; 2) estimation of chemical abundances of nova ejecta from coronal and other emission line spectroscopy; and 3) measurement of the density and masses of the ejecta. This Gemini `Target of Opportunity' initiative (trigger K=5- 8 mag, assuming adequate PWFS guide stars exist) complements our extensive Spitzer, Chandra, Swift, XMM-Newton CN DDT/ToO programs.

  7. Collapsing vortex filaments and the spectrum of quantum turbulence (United States)

    Andryushchenko, V. A.; Nemirovskii, S. K.


    The method of correlation functions and the method of quantum vortex configurations are used to calculate the energy spectrum of a three-dimensional velocity field that is induced by collapsing (immediately before reconnection) vortex filaments. The formulation of this problem is motivated by the idea of modeling classical turbulence by a set of chaotic quantized vortex filaments. Among the various arguments that support the idea of quasi-classical behavior for quantum turbulence, the most persuasive is probably the resulting Kolmogorov energy spectrum resembling E ( k ) ∝ k - 5 / 3 that was obtained in a number of numerical studies. Another goal is associated with an important and intensely studied theme that relates to the role of hydrodynamic collapse in the formation of turbulence spectra. Calculations have demonstrated that vortex filaments create a velocity field at the moment of contact, which has a singularity. This configuration of vortex filaments generates the spectrum E(k), which bears the resemblance to the Kolmogorov law. A possible cause for this observation is discussed, as well as the likely reasons behind any deviations. The obtained results are discussed from the perspective of both classical and quantum turbulence.

  8. Effects of a mixture of tetracyclines to Lemna gibba and Myriophyllum sibiricum evaluated in aquatic microcosms

    Energy Technology Data Exchange (ETDEWEB)

    Brain, Richard A [Centre for Toxicology, Department of Environmental Biology, University of Guelph, Guelph, Ontario, N1G 2W1 (Canada); Wilson, Christian J [Centre for Toxicology, Department of Environmental Biology, University of Guelph, Guelph, Ontario, N1G 2W1 (Canada); Johnson, David J [Centre for Toxicology, Department of Environmental Biology, University of Guelph, Guelph, Ontario, N1G 2W1 (Canada); Sanderson, Hans [Centre for Toxicology, Department of Environmental Biology, University of Guelph, Guelph, Ontario, N1G 2W1 (Canada); Bestari, Ketut [Centre for Toxicology, Department of Environmental Biology, University of Guelph, Guelph, Ontario, N1G 2W1 (Canada); Hanson, Mark L [Centre for Toxicology, Department of Environmental Biology, University of Guelph, Guelph, Ontario, N1G 2W1 (Canada); Sibley, Paul K [Centre for Toxicology, Department of Environmental Biology, University of Guelph, Guelph, Ontario, N1G 2W1 (Canada); Solomon, Keith R [Centre for Toxicology, Department of Environmental Biology, University of Guelph, Guelph, Ontario, N1G 2W1 (Canada)


    The impact of a mixture of oxytetracycline, chlortetracycline, tetracycline and doxycycline on Myriophyllum sibiricum and Lemna gibba was investigated using fifteen 12,000-L microcosms (k = 5, n = 3). Significant concentration-response relationships were only found for M. sibiricum, where dry mass was 69, 47, 30, and 7% of controls at respective treatment concentrations of 0.080, 0.218, 0.668, and 2.289 {mu}mol/L. Somatic endpoints were strongly and negatively correlated with percent light transmission, except plant length, which was positively correlated. Treated microcosms experienced a reduction in the percent of surface irradiance penetrating the water column as high as 99.8% at a depth of 70 cm, relative to controls. Position relative to the water column was likely responsible for the differential effects observed between floating (L. gibba) and submerged (M. sibiricum) species of macrophytes. A hazard quotient assessment of the lowest EC{sub 10} value indicated significant risk, exceeding the critical HQ value, but not the lowest EC{sub 25} value. - Mixtures of tetracycline antibiotics pose a risk to submerged but not floating aquatic plants.

  9. Observation of B Meson Decays to omegapi+, omegaK+, and omegaK0

    International Nuclear Information System (INIS)

    Smith, James G


    The authors present preliminary measurements of B meson decays to B + → ωπ + , B + → ωK + , and B 0 → ωK 0 . The data were recorded with the BABAR detector and correspond to 88.9 x 10 6 B(bar B) pairs produced in e + e - annihilation at the Υ(4S) resonance. They find statistically significant signals for all three channels: Β(B + → ωπ + ) = (5.4 ± 1.0 ± 0.5) x 10 -6 , Β(B + → ωK + ) = (5.0 ± 1.0 ± 0.4) x 10 -6 , and Β(B 0 → ωK 0 ) = (5.3 -1.2 +1.4 ± 0.5) x 10 -6 . They also measure time-integrated charge asymmetries Α ch (B + → ωπ + ) = 0.04 ± 0.17 ± 0.01 and Α ch (B + → ωK + ) = -0.05 ± 0.16 ± 0.01

  10. Requirements within the Ebola Viral Glycoprotein for Tetherin Antagonism

    Directory of Open Access Journals (Sweden)

    Nathan H. Vande Burgt


    Full Text Available Tetherin is an interferon-induced, intrinsic cellular response factor that blocks release of numerous viruses, including Ebola virus, from infected cells. As with many viruses targeted by host factors, Ebola virus employs a tetherin antagonist, the viral glycoprotein (EboGP, to counteract restriction and promote virus release. Unlike other tetherin antagonists such as HIV-1 Vpu or KSHV K5, the features within EboGP needed to overcome tetherin are not well characterized. Here, we describe sequences within the EboGP ectodomain and membrane spanning domain (msd as necessary to relieve tetherin restriction of viral particle budding. Fusing the EboGP msd to a normally secreted form of the glycoprotein effectively promotes Ebola virus particle release. Cellular protein or lipid anchors could not substitute for the EboGP msd. The requirement for the EboGP msd was not specific for filovirus budding, as similar results were seen with HIV particles. Furthermore trafficking of chimeric proteins to budding sites did not correlate with an ability to counter tetherin. Additionally, we find that a glycoprotein construct, which mimics the cathepsin-activated species by proteolytic removal of the EboGP glycan cap and mucin domains, is unable to counteract tetherin. Combining these results suggests an important role for the EboGP glycan cap and msd in tetherin antagonism.

  11. A Novel Study of Methane-Rich Gas Reforming to Syngas and Its Kinetics over Semicoke Catalyst

    Directory of Open Access Journals (Sweden)

    Guojie Zhang


    Full Text Available A small-size gasification unit is improved through process optimization to simulate industrial United Gas Improvement Company gasification. It finds that the reaction temperature has important impacts on semicoke catalyzed methane gas mixture. The addition of water vapor can enhance the catalytic activity of reforming, which is due to the fact that addition of water vapor not only removes carbon deposit produced in the reforming and gasification reaction processes, but also participates in gasification reaction with semicoke to generate some active oxygen-containing functional groups. The active oxygen-containing functional groups provide active sites for carbon dioxide reforming of methane, promoting the reforming reaction. It also finds that the addition of different proportions of methane-rich gas can yield synthesis gas with different H2/CO ratio. The kinetics study shows that the semicoke can reduce the activation energy of the reforming reaction and promote the occurrence of the reforming reaction. The kinetics model of methane reforming under the conditions of steam gasification over semicoke is as follows: k-=5.02×103·pCH40.71·pH20.26·exp(−74200/RT.

  12. Chronic Proliferative Dermatitis in Mice: NFκB Activation Autoinflammatory Disease

    Directory of Open Access Journals (Sweden)

    Yanhua Liang


    Full Text Available Autoinflammatory diseases are a heterogeneous group of congenital diseases characterized by the presence of recurrent inflammation, in the absence of infectious agents, detectable autoantibodies or antigen-specific autoreactive T-cells. SHARPIN deficient mice presents multiorgan chronic inflammation without known autoantibodies or autoreactive T-cells, designated Sharpincpdm. Histological studies demonstrated epidermal hyperproliferation, Th-2 inflammation, and keratinocyte apoptosis in this mutant. The mutant mice have decreased behavioral mobility, slower growth, and loss of body weight. Epidermal thickness and mitotic epidermal cells increase along with disease development. K5/K14 expression is distributed through all layers of epidermis, along with K6 expression in interfollicular epidermis, suggesting epidermal hyperproliferation. K1/K10 is only detectable in outer layers of spinosum epidermis, reflecting accelerated keratinocyte migration. Alpha smooth muscle actin is overexpressed in skin blood vessels, which may release the elevated white blood cells to dermis. CD3+CD45+ cells and granulocytes, especially eosinophils and mast cells, aggregate in the mutant skin. TUNEL assay, together with Annexin-V/propidium iodide FACS analysis, confirmed the increase of apoptotic keratinocytes in skin. These data validate and provide new lines of evidence of the proliferation-inflammation-apoptosis triad in Sharpincpdm mice, an NFκB activation autoinflammatory disease.

  13. Improving adoption and acceptability of digital health interventions for HIV disease management: a qualitative study. (United States)

    Claborn, Kasey R; Meier, Ellen; Miller, Mary Beth; Leavens, Eleanor L; Brett, Emma I; Leffingwell, Thad


    Disease management remains a challenge for many people living with HIV (PLWH). Digital health interventions (DHIs) may assist with overcoming these challenges and reducing burdens on clinical staff; however, there is limited data regarding methods to improve uptake and acceptability of DHIs among PLWH. This qualitative study aimed to assess patient and provider perspectives on the use of DHIs and strategies to promote uptake among PLWH. Eight focus groups with patients (k = 5 groups; n = 24) and providers (k = 3 groups; n = 12) were conducted May through October of 2014. Focus groups (~90 min) followed a semi-structured interview guide. Data were analyzed using thematic analysis on three main themes: (a) perspectives towards the adoption and use of DHIs for HIV management; (b) perceptions of barriers and facilitators to patient usage; and (c) preferences regarding content, structure, and delivery. Analyses highlighted barriers and facilitators to DHI adoption. Patients and providers agreed that DHIs feel "impersonal" and "lack empathy," may be more effective for certain subpopulations, should be administered in the clinic setting, and should use multimodal delivery methods. Emergent themes among the providers included development of DHIs for providers as the target market and the need for culturally adapted DHIs for patient subpopulations. DHIs have potential to improve HIV management and health outcomes. DHIs should be developed in conjunction with anticipated consumers, including patients, providers, and other key stakeholders. DHIs tailored for specific HIV subpopulations are needed. Future studies should evaluate dissemination methods and marketing strategies to promote uptake.

  14. ABILITY OF BACTERIAL CONSORTIUM: Bacillus coagulans, Bacilus licheniformis, Bacillus pumilus, Bacillus subtilis, Nitrosomonas sp. and Pseudomonas putida IN BIOREMEDIATION OF WASTE WATER IN CISIRUNG WASTE WATER TREATMENT PLANT

    Directory of Open Access Journals (Sweden)

    Ratu SAFITRI


    Full Text Available This study was conducted in order to determine the ability of bacterial consortium: Bacillus coagulans, Bacilus licheniformis, Bacillus pumilus, Bacillus subtilis, Nitrosomonas sp., and Pseudomonas putida in bioremediation of wastewater origin Cisirung WWTP. This study uses an experimental method completely randomized design (CRD, which consists of two treatment factors (8x8 factorial design. The first factor is a consortium of bacteria (K, consisting of 8 level factors (k1, k2, k3, k4, k5, k6, k7, and k8. The second factor is the time (T, consisting of a 7 level factors (t0, t1, t2, t3, t4, t5, t6, and t7. Test parameters consist of BOD (Biochemical Oxygen Demand, COD (Chemical Oxygen Demand, TSS (Total Suspended Solid, Ammonia and Population of Microbes during bioremediation. Data were analyzed by ANOVA, followed by Duncan test. The results of this study showed that the consortium of Bacillus pumilus, Bacillus subtilis, Bacillus coagulans, Nitrosomonas sp., and Pseudomonas putida with inoculum concentration of 5% (k6 is a consortium of the most effective in reducing BOD 71.93%, 64.30% COD, TSS 94.85%, and 88.58% of ammonia.

  15. Selective Oxidation and Reactive Wetting during Galvanizing of a CMnAl TRIP-Assisted Steel (United States)

    Bellhouse, E. M.; McDermid, J. R.


    A transformation induced plasticity (TRIP)-assisted steel with 0.2 pct C, 1.5 pct Mn, and 1.5 pct Al was successfully galvanized using a thermal cycle previously shown to produce an excellent combination of strength and ductility. The steel surface chemistry and oxide morphology were determined as a function of process atmosphere oxygen partial pressure. For the 220 K (-53 °C) dew point (dp) + 20 pct H2 atmosphere, the oxide morphology was a mixture of films and nodules. For the 243 K (-30 °C) dp + 5 pct H2 atmosphere, nodules of MnO were found primarily at grain boundaries. For the 278 K (+5 °C) dp + 5 pct H2 atmosphere, nodules of metallic Fe were found on the surface as a result of alloy element internal oxidation. The steel surface chemistry and oxide morphology were then related to the reactive wetting behavior during continuous hot dip galvanizing. Good wetting was obtained using the two lower oxygen partial pressure process atmospheres [220 K dp and 243 K dp (-53 °C dp and -30 °C dp)]. An increase in the number of bare spots was observed when using the higher oxygen partial pressure process atmosphere (+5 °C dp) due to the increased thickness of localized oxide films.

  16. Calculations of flow oscillations during reflood using RELAP4/MOD6

    International Nuclear Information System (INIS)

    Chen, Y.S.; Fischer, S.R.; Sullivan, L.H.


    RELAP4/MOD6 is an analytical computer code which can be used for best-estimate analysis of LWR reactor system blowdown and reflood response to a postulated LOCA. In this study, flow oscillations in the PKL reflood test K5A were investigated using RELAP4/MOD6. Both calculated and measured oscillations exhibited transient characteristics of density-wave and pressure-drop oscillations. The calculated average core mixture level rising rate agrees closely with the test data. Several mechanisms which appear to be responsible for initiation and continuation of calculated or experimental reflood flow oscillations are (a) the coupling between the vapor generation in the core channel and the U-tube geometrical arrangement of a downcomer and a heated core; (b) the inherent low core inlet resistance and the high system outlet resistance; (c) the dependence of heat transfer rate on mass flow rate especially in the dispersed flow ially in the dispersed flow regime; (d) the amount of the liquid entrainment fraction of the heated core channel

  17. Photometric Survey to Search for Field sdO Pulsators (United States)

    Johnson, C.; Green, E.; Wallace, S.; O'Malley, C.; Amaya, H.; Biddle, L.; Fontaine, G.


    We present the results of a campaign to search for subdwarf O (sdO) star pulsators among bright field stars. The motivation for this project is the recent discovery by Randall et al. (2011) of four rapidly pulsating sdO stars in the globular cluster ω Cen, with Teff near 50,000 K, 5.4 -0.1 and similar temperatures and gravities. To date, we have found no detectable pulsations at amplitudes above 0.08% (4 times the mean noise level) in any of the 36 field sdO stars that we observed. The presence of pulsations in ω Cen sdO stars and their apparent absence in seemingly comparable field sdO stars is perplexing. While very suggestive, the significance of this result is difficult to assess more completely right now due to remaining uncertainties about the temperature width and purity of the ω Cen instability strip and the existence of any sdO pulsators with weaker amplitudes than the current detection limit in globular clusters.

  18. Inhibition of apoptosis signal-regulating kinase 1 alters the wound epidermis and enhances auricular cartilage regeneration.

    Directory of Open Access Journals (Sweden)

    Qian-Shi Zhang

    Full Text Available Why regeneration does not occur in mammals remains elusive. In lower vertebrates, epimorphic regeneration of the limb is directed by the wound epidermis, which controls blastema formation to promote regrowth of the appendage. Herein, we report that knockout (KO or inhibition of Apoptosis Signal-regulated Kinase-1 (ASK1, also known as mitogen-activated protein kinase kinase kinase 5 (MAP3K5, after full thickness ear punch in mice prolongs keratinocyte activation within the wound epidermis and promotes regeneration of auricular cartilage. Histological analysis showed the ASK1 KO ears displayed enhanced protein markers associated with blastema formation, hole closure and regeneration of auricular cartilage. At seven days after punch, the wound epidermis morphology was markedly different in the KO, showing a thickened stratum corneum with rounded cell morphology and a reduction of both the granular cell layer and decreased expression of filament aggregating protein. In addition, cytokeratin 6 was expressed in the stratum spinosum and granulosum. Topical application of inhibitors of ASK1 (NQDI-1, the upstream ASK1 activator, calcium activated mitogen kinase 2 (KN93, or the downstream target, c-Jun N-terminal kinase (SP600125 also resulted in enhanced regeneration; whereas inhibition of the other downstream target, the p38 α/β isoforms, (SB203580 had no effect. The results of this investigation indicate ASK1 inhibition prolongs keratinocyte and blastemal cell activation leading to ear regeneration.

  19. Inhibition of apoptosis signal-regulating kinase 1 alters the wound epidermis and enhances auricular cartilage regeneration (United States)

    Zhang, Qian-Shi; Kurpad, Deepa S.; Mahoney, My G.; Steinbeck, Marla J.


    Why regeneration does not occur in mammals remains elusive. In lower vertebrates, epimorphic regeneration of the limb is directed by the wound epidermis, which controls blastema formation to promote regrowth of the appendage. Herein, we report that knockout (KO) or inhibition of Apoptosis Signal-regulated Kinase-1 (ASK1), also known as mitogen-activated protein kinase kinase kinase 5 (MAP3K5), after full thickness ear punch in mice prolongs keratinocyte activation within the wound epidermis and promotes regeneration of auricular cartilage. Histological analysis showed the ASK1 KO ears displayed enhanced protein markers associated with blastema formation, hole closure and regeneration of auricular cartilage. At seven days after punch, the wound epidermis morphology was markedly different in the KO, showing a thickened stratum corneum with rounded cell morphology and a reduction of both the granular cell layer and decreased expression of filament aggregating protein. In addition, cytokeratin 6 was expressed in the stratum spinosum and granulosum. Topical application of inhibitors of ASK1 (NQDI-1), the upstream ASK1 activator, calcium activated mitogen kinase 2 (KN93), or the downstream target, c-Jun N-terminal kinase (SP600125) also resulted in enhanced regeneration; whereas inhibition of the other downstream target, the p38 α/β isoforms, (SB203580) had no effect. The results of this investigation indicate ASK1 inhibition prolongs keratinocyte and blastemal cell activation leading to ear regeneration. PMID:29045420


    International Nuclear Information System (INIS)

    Nowak, G.; Niedzielski, A.; Adamów, M.; Maciejewski, G.; Wolszczan, A.


    We report the discovery of planetary-mass companions to two red giants by the ongoing Penn State-Toruń Planet Search (PTPS) conducted with the 9.2 m Hobby-Eberly Telescope. The 1.1 M ☉ K0-giant, BD+15 2940, has a 1.1 M J minimum mass companion orbiting the star at a 137.5 day period in a 0.54 AU orbit what makes it the closest—in planet around a giant and possible subject of engulfment as the consequence of stellar evolution. HD 233604, a 1.5 M ☉ K5-giant, is orbited by a 6.6 M J minimum mass planet which has a period of 192 days and a semi-major axis of only 0.75 AU making it one of the least distant planets to a giant star. The chemical composition analysis of HD 233604 reveals a relatively high 7 Li abundance which may be a sign of its early evolutionary stage or recent engulfment of another planet in the system. We also present independent detections of planetary-mass companions to HD 209458 and HD 88133, and stellar activity-induced radial velocity variations in HD 166435, as part of the discussion of the observing and data analysis methods used in the PTPS project.

  1. Modulation of the unpaired spin localization in Pentavalent Uranyl Complexes

    Energy Technology Data Exchange (ETDEWEB)

    Vetere, V.; Maldivi, P.; Mazzanti, M. [CEA Grenoble, INAC, SCIB, laboratoire de reconnaissance ionique et chimie de coordination, 38 (France); Vetere, V. [UMR5626, laboratoire de chimie et physique quantique, universite de Toulouse, 31 - Toulouse (France)


    The electronic structure of various complexes of pentavalent uranyl species, namely UO{sub 2}{sup +}, is described, using DFT methods, with the aim of understanding how the structure of the ligands may influence the localisation of the unpaired 5f electron of uranium (V) and, finally, the stability of such complexes towards oxidation. Six complexes have been inspected: [UO{sub 2}py{sub 5}]{sup +} (1), [(UO{sub 2}py{sub 5})KI{sub 2}] (2), [UO{sub 2}(salan-{sup t}Bu{sub 2})(py)K] (3), [UO{sub 2}(salophen-{sup t}Bu{sub 2})(thf)K] (4), [UO{sub 2}(salen-{sup t}Bu{sub 2})(py)K] (5), [and UO{sub 2}-cyclo[6]pyrrole]{sup 1-} (6), chosen to explore various ligands. In the five first complexes, the UO{sub 2}{sup +} species is well identified with the unpaired electron localized on the 5f uranium orbital. Additionally, for the salan, salen and salophen ligands, some covalent interactions have been observed, resulting from the presence of both donor and acceptor binding sites. In contrast, the last complex is best described by a UO{sub 2}{sup 2+} uranyl (VI) coordinated by the anionic radical cyclo-pyrrole, the highly delocalized p orbitals set stabilizing the radical behaviour of this ligand. (authors)


    International Nuclear Information System (INIS)

    Hunana, P.; Zank, G. P.


    The nearly incompressible theory of magnetohydrodynamics (MHD) is formulated in the presence of a static large-scale inhomogeneous background. The theory is an inhomogeneous generalization of the homogeneous nearly incompressible MHD description of Zank and Matthaeus and a polytropic equation of state is assumed. The theory is primarily developed to describe solar wind turbulence where the assumption of a composition of two-dimensional (2D) and slab turbulence with the dominance of the 2D component has been used for some time. It was however unclear, if in the presence of a large-scale inhomogeneous background, the dominant component will also be mainly 2D and we consider three distinct MHD regimes for the plasma beta β > 1. For regimes appropriate to the solar wind (β 2 s δp is not valid for the leading-order O(M) density fluctuations, and therefore in observational studies, the density fluctuations should not be analyzed through the pressure fluctuations. The pseudosound relation is valid only for higher order O(M 2 ) density fluctuations, and then only for short-length scales and fast timescales. The spectrum of the leading-order density fluctuations should be modeled as k -5/3 in the inertial range, followed by a Bessel function solution K ν (k), where for stationary turbulence ν = 1, in the viscous-convective and diffusion range. Other implications for solar wind turbulence with an emphasis on the evolution of density fluctuations are also discussed.

  3. Potassium and calcium application ameliorates growth and oxidative homeostasis in salt-stressed indian mustard (brassica juncea) plants

    International Nuclear Information System (INIS)

    Yousuf, P. Y.; Ahmad, A.; Hemant, M.; Ganie, A. H.; Iqbal, M.; Aref, I. M.


    The effect of potassium (K) and calcium (Ca) on growth and antioxidant defence system of salt-stressed Indian mustard plants was studied. Twenty-day-old Indian mustard plants grown hydroponically in Hoagland growth medium were randomly divided into five groups. To served as control and did not receive any additional K or Ca (except that present in Hoagland solution), T1 received 150 mM NaCl, T2 was given an additional doze of 6 mM K, T3 was given 5.6 mM Ca as additional doze, while as T4 received a combination of 150 mM NaCl + 6 mM K + 5.6 mM Ca. The response of the plants was studied ten days after treatment. Salt stress inhibited growth parameters including biomass, chlorophyll content, protein content and NR activity. Membrane damage was induced by the salt treatment with a concurrent increase in antioxidant defence system and proline content. Individual application of K and Ca mitigated the negative influence of the stress with the maximum alleviating potential exhibited by the combined application of these nutrients. Results obtained on real time expression of genes encoding enzymatic antioxidants (SOD, APX, CAT and GR), NR and proline supported our findings with biochemical assays. We conclude from the study that maintaining high K and Ca levels may serve as an effective means for regulating the growth and productivity of Indian mustard plants under saline conditions. (author)


    International Nuclear Information System (INIS)

    Santerne, A.; Barros, S. C. C.; Mena, E. Delgado; Montalto, M.; Sousa, S. G.; Adibekyan, V.; Hébrard, G.; Lillo-Box, J.; Barrado, D.; Armstrong, D. J.; Pollacco, D.; Osborn, H. P.; Demangeon, O.; Deleuil, M.; Debackere, A.; Arlic, G.; Barthe, G.; Abe, L.; Almenara, J.-M.; André, P.


    In the present paper we report the discovery of a new hot Jupiter, K2-29 b, first detected by the Super-WASP observatory and then by the K2 space mission during its campaign 4. The planet has a period of 3.25 days, a mass of 0.73 ± 0.04 M ♃ , and a radius of 1.19 ± 0.02 R ♃ . The host star is a relatively bright ( V = 12.5) G7 dwarf with a nearby K5V companion. Based on stellar rotation and the abundance of lithium, we find that the system might be as young as ∼450 Myr. The observation of the Rossiter–McLaughlin effect shows that the planet is aligned with respect to the stellar spin. Given the deep transit (20 mmag), the magnitude of the star and the presence of a nearby stellar companion, the planet is a good target for both space- and ground-based transmission spectroscopy, in particular in the near-infrared where both stars are relatively bright.

  5. Energy spectrum of buoyancy-driven turbulence

    KAUST Repository

    Kumar, Abhishek


    Using high-resolution direct numerical simulation and arguments based on the kinetic energy flux Πu, we demonstrate that, for stably stratified flows, the kinetic energy spectrum Eu(k)∼k-11/5, the potential energy spectrum Eθ(k)∼k-7/5, and Πu(k)∼k-4/5 are consistent with the Bolgiano-Obukhov scaling. This scaling arises due to the conversion of kinetic energy to the potential energy by buoyancy. For weaker buoyancy, this conversion is weak, hence Eu(k) follows Kolmogorov\\'s spectrum with a constant energy flux. For Rayleigh-Bénard convection, we show that the energy supply rate by buoyancy is positive, which leads to an increasing Πu(k) with k, thus ruling out Bolgiano-Obukhov scaling for the convective turbulence. Our numerical results show that convective turbulence for unit Prandt number exhibits a constant Πu(k) and Eu(k)∼k-5/3 for a narrow band of wave numbers. © 2014 American Physical Society.

  6. Different phases of a system of hard rods on three dimensional cubic lattice (United States)

    Vigneshwar, N.; Dhar, Deepak; Rajesh, R.


    We study the different phases of a system of monodispersed hard rods of length k on a cubic lattice, using an efficient cluster algorithm able to simulate densities close to the fully-packed limit. For k≤slant 4 , the system is disordered at all densities. For k=5, 6 , we find a single density-driven transition, from a disordered phase to high density layered-disordered phase, in which the density of rods of one orientation is strongly suppressed, breaking the system into weakly coupled layers. Within a layer, the system is disordered. For k ≥slant 7 , three density-driven transitions are observed numerically: isotropic to nematic to layered-nematic to layered-disordered. In the layered-nematic phase, the system breaks up into layers, with nematic order in each layer, but very weak correlation between the ordering directions of different layers. We argue that the layered-nematic phase is a finite-size effect, and in the thermodynamic limit, the nematic phase will have higher entropy per site. We expect the systems of rods in four and higher dimensions will have a qualitatively similar phase diagram.


    International Nuclear Information System (INIS)

    Tang Sumin; Grindlay, Jonathan; Los, Edward; Servillat, Mathieu


    KU Cyg is an eclipsing binary consisting of an F-type star accreting through a large accretion disk from a K5III red giant. Here we present the discovery of a 5 year dip around 1900 found from its 100 year DASCH light curve. It showed a ∼0.5 mag slow fading from 1899 to 1903 and brightened back around 1904 on a relatively shorter timescale. The light curve shape of the 1899-1904 fading-brightening event differs from the dust production and dispersion process observed in R Coronae Borealis stars, which usually has a faster fading and slower recovery, and for KU Cyg is probably related to the accretion disk surrounding the F star. The slow fading in KU Cyg is probably caused by increases in dust extinction in the disk, and the subsequent quick brightening may be due to the evaporation of dust transported inward through the disk. The extinction excess which caused the fading may arise from increased mass transfer rate in the system or from dust clump ejections from the K giant.

  8. Reduction of Gas Bubbles and Improved Critical Current Density in Bi-2212 Round Wire by Swaging

    CERN Document Server

    Jiang, J; Huang, Y; Hong, S; Parrell, J; Scheuerlein, C; Di Michiel, M; Ghosh, A; Trociewitz, U; Hellstrom, E; Larbalestier, D


    Bi-2212 round wire is made by the powder-in-tube technique. An unavoidable property of powder-in-tube conductors is that there is about 30% void space in the as-drawn wire. We have recently shown that the gas present in the as-drawn Bi-2212 wire agglomerates into large bubbles and that they are presently the most deleterious current limiting mechanism. By densifying short 2212 wires before reaction through cold isostatic pressing (CIPping), the void space was almost removed and the gas bubble density was reduced significantly, resulting in a doubled engineering critical current density (JE) of 810 A/mm2 at 5 T, 4.2 K. Here we report on densifying Bi-2212 wire by swaging, which increased JE (4.2 K, 5 T) from 486 A/mm2 for as-drawn wire to 808 A/mm2 for swaged wire. This result further confirms that enhancing the filament packing density is of great importance for making major JE improvement in this round-wire magnet conductor.

  9. The CARMENES search for exoplanets around M dwarfs. HD147379 b: A nearby Neptune in the temperate zone of an early-M dwarf (United States)

    Reiners, A.; Ribas, I.; Zechmeister, M.; Caballero, J. A.; Trifonov, T.; Dreizler, S.; Morales, J. C.; Tal-Or, L.; Lafarga, M.; Quirrenbach, A.; Amado, P. J.; Kaminski, A.; Jeffers, S. V.; Aceituno, J.; Béjar, V. J. S.; Guàrdia, J.; Guenther, E. W.; Hagen, H.-J.; Montes, D.; Passegger, V. M.; Seifert, W.; Schweitzer, A.; Cortés-Contreras, M.; Abril, M.; Alonso-Floriano, F. J.; Eiff, M. Ammler-von; Antona, R.; Anglada-Escudé, G.; Anwand-Heerwart, H.; Arroyo-Torres, B.; Azzaro, M.; Baroch, D.; Barrado, D.; Bauer, F. F.; Becerril, S.; Benítez, D.; Berdiñas, Z. M.; Bergond, G.; Blümcke, M.; Brinkmöller, M.; del Burgo, C.; Cano, J.; Cárdenas Vázquez, M. C.; Casal, E.; Cifuentes, C.; Claret, A.; Colomé, J.; Czesla, S.; Díez-Alonso, E.; Feiz, C.; Fernández, M.; Ferro, I. M.; Fuhrmeister, B.; Galadí-Enríquez, D.; Garcia-Piquer, A.; García Vargas, M. L.; Gesa, L.; Gómez Galera, V.; González Hernández, J. I.; González-Peinado, R.; Grözinger, U.; Grohnert, S.; Guijarro, A.; de Guindos, E.; Gutiérrez-Soto, J.; Hatzes, A. P.; Hauschildt, P. H.; Hedrosa, R. P.; Helmling, J.; Henning, Th.; Hermelo, I.; Hernández Arabí, R.; Hernández Castaño, L.; Hernández Hernando, F.; Herrero, E.; Huber, A.; Huke, P.; Johnson, E. N.; de Juan, E.; Kim, M.; Klein, R.; Klüter, J.; Klutsch, A.; Kürster, M.; Labarga, F.; Lamert, A.; Lampón, M.; Lara, L. M.; Laun, W.; Lemke, U.; Lenzen, R.; Launhardt, R.; López del Fresno, M.; López-González, M. J.; López-Puertas, M.; López Salas, J. F.; López-Santiago, J.; Luque, R.; Magán Madinabeitia, H.; Mall, U.; Mancini, L.; Mandel, H.; Marfil, E.; Marín Molina, J. A.; Maroto Fernández, D.; Martín, E. L.; Martín-Ruiz, S.; Marvin, C. J.; Mathar, R. J.; Mirabet, E.; Moreno-Raya, M. E.; Moya, A.; Mundt, R.; Nagel, E.; Naranjo, V.; Nortmann, L.; Nowak, G.; Ofir, A.; Oreiro, R.; Pallé, E.; Panduro, J.; Pascual, J.; Pavlov, A.; Pedraz, S.; Pérez-Calpena, A.; Pérez Medialdea, D.; Perger, M.; Perryman, M. A. C.; Pluto, M.; Rabaza, O.; Ramón, A.; Rebolo, R.; Redondo, P.; Reffert, S.; Reinhart, S.; Rhode, P.; Rix, H.-W.; Rodler, F.; Rodríguez, E.; Rodríguez-López, C.; Rodríguez Trinidad, A.; Rohloff, R.-R.; Rosich, A.; Sadegi, S.; Sánchez-Blanco, E.; Sánchez Carrasco, M. A.; Sánchez-López, A.; Sanz-Forcada, J.; Sarkis, P.; Sarmiento, L. F.; Schäfer, S.; Schmitt, J. H. M. M.; Schiller, J.; Schöfer, P.; Solano, E.; Stahl, O.; Strachan, J. B. P.; Stürmer, J.; Suárez, J. C.; Tabernero, H. M.; Tala, M.; Tulloch, S. M.; Ulbrich, R.-G.; Veredas, G.; Vico Linares, J. I.; Vilardell, F.; Wagner, K.; Winkler, J.; Wolthoff, V.; Xu, W.; Yan, F.; Zapatero Osorio, M. R.


    We report on the first star discovered to host a planet detected by radial velocity (RV) observations obtained within the CARMENES survey for exoplanets around M dwarfs. HD 147379 (V = 8.9 mag, M = 0.58 ± 0.08 M⊙), a bright M0.0 V star at a distance of 10.7 pc, is found to undergo periodic RV variations with a semi-amplitude of K = 5.1 ± 0.4 m s-1 and a period of P = 86.54 ± 0.06 d. The RV signal is found in our CARMENES data, which were taken between 2016 and 2017, and is supported by HIRES/Keck observations that were obtained since 2000. The RV variations are interpreted as resulting from a planet of minimum mass mP sin i = 25 ± 2 M⊕, 1.5 times the mass of Neptune, with an orbital semi-major axis a = 0.32 au and low eccentricity (e or via


    Energy Technology Data Exchange (ETDEWEB)

    Qian, S. B.; Zhang, B.; He, J. J.; Liu, L.; Zhu, L. Y.; Zhao, E. G.; Zhou, X.; Thawicharat, S. [Yunnan Observatories, Chinese Academy of Sciences (CAS), P.O. Box 110, 650011 Kunming (China); Soonthornthum, B.; Rattanasoon, S.; Aukkaravittayapun, S., E-mail: [National Astronomical Research Institute of Thailand, 191 Siriphanich Bldg., Huay Kaew Road, Chiang Mai 50200 (Thailand)


    SuperWASP J015100.23-100524.2 (hereafter J015100) is an eclipsing binary with an orbital period of 0.d2145 that is below the short-period limit of contact binary stars. Complete light curves of J015100 in B, V, R, and I bands are presented and are analyzed with the Wilson–Devinney method. It has been discovered that J015100 is a shallow-contact binary (f = 14.6(±2.7)%) with a mass ratio of 3.128. It is a W-type contact binary where the less massive component is about 130 K hotter than the more massive one. The asymmetries of light curves are explained as one dark spot on the more massive component. The detection of J015100 as a contact binary below the period limit suggests that contact binaries below this limit are not rapidly destroyed. This shallow-contact system may be formed from a detached short-period binary similar to DV Psc (Sp. = K4/K5; P = 0.d30855) via orbital shrinkage due to angular momentum loss through magnetic stellar wind.

  11. Complex network analysis of state spaces for random Boolean networks

    Energy Technology Data Exchange (ETDEWEB)

    Shreim, Amer [Complexity Science Group, Department of Physics and Astronomy, University of Calgary, Calgary, AB, T2N 1N4 (Canada); Berdahl, Andrew [Complexity Science Group, Department of Physics and Astronomy, University of Calgary, Calgary, AB, T2N 1N4 (Canada); Sood, Vishal [Complexity Science Group, Department of Physics and Astronomy, University of Calgary, Calgary, AB, T2N 1N4 (Canada); Grassberger, Peter [Complexity Science Group, Department of Physics and Astronomy, University of Calgary, Calgary, AB, T2N 1N4 (Canada); Paczuski, Maya [Complexity Science Group, Department of Physics and Astronomy, University of Calgary, Calgary, AB, T2N 1N4 (Canada)


    We apply complex network analysis to the state spaces of random Boolean networks (RBNs). An RBN contains N Boolean elements each with K inputs. A directed state space network (SSN) is constructed by linking each dynamical state, represented as a node, to its temporal successor. We study the heterogeneity of these SSNs at both local and global scales, as well as sample to-sample fluctuations within an ensemble of SSNs. We use in-degrees of nodes as a local topological measure, and the path diversity (Shreim A et al 2007 Phys. Rev. Lett. 98 198701) of an SSN as a global topological measure. RBNs with 2 {<=} K {<=} 5 exhibit non-trivial fluctuations at both local and global scales, while K = 2 exhibits the largest sample-to-sample (possibly non-self-averaging) fluctuations. We interpret the observed 'multi scale' fluctuations in the SSNs as indicative of the criticality and complexity of K = 2 RBNs. 'Garden of Eden' (GoE) states are nodes on an SSN that have in-degree zero. While in-degrees of non-GoE nodes for K > 1 SSNs can assume any integer value between 0 and 2{sup N}, for K = 1 all the non-GoE nodes in a given SSN have the same in-degree which is always a power of two.

  12. Nascent chromatin capture proteomics determines chromatin dynamics during DNA replication and identifies unknown fork components

    DEFF Research Database (Denmark)

    Alabert, Constance; Bukowski-Wills, Jimi-Carlo; Lee, Sung-Po


    To maintain genome function and stability, DNA sequence and its organization into chromatin must be duplicated during cell division. Understanding how entire chromosomes are copied remains a major challenge. Here, we use nascent chromatin capture (NCC) to profile chromatin proteome dynamics during...... replication in human cells. NCC relies on biotin-dUTP labelling of replicating DNA, affinity purification and quantitative proteomics. Comparing nascent chromatin with mature post-replicative chromatin, we provide association dynamics for 3,995 proteins. The replication machinery and 485 chromatin factors...... such as CAF-1, DNMT1 and SUV39h1 are enriched in nascent chromatin, whereas 170 factors including histone H1, DNMT3, MBD1-3 and PRC1 show delayed association. This correlates with H4K5K12diAc removal and H3K9me1 accumulation, whereas H3K27me3 and H3K9me3 remain unchanged. Finally, we combine NCC enrichment...

  13. Thermal expansion of NZP-family alkali-metal (Na, K) zirconium phosphates

    International Nuclear Information System (INIS)

    Orlova, A.I.; Kemenov, D.V.; Pet'kov, V.I.; Samojlov, S.G.; Kazantsev, G.N.


    By means of high-temperature X-ray diffraction one investigated into thermal expansion of alkali-zirconium phosphates crystallizing in NaZr 2 (PO 4 ) 3 structure type within 20-700 deg C temperature range. One synthesized phosphates of A x Zr 2.25-0.25x (PO 4 ) 3 type two series where A-Na (x = 0.5; 1.0; 2.0; 3.0; 4.0; 5.0) and K (x = 1.0; 3.0; 5.0). One calculated for them a and c parameters of the elementary cells and α a and α c linear expansion temperature coefficients. Anisotropy of thermal expansion the maximum one for AZr 2 (PO 4 ) 3 and Na 5 Zr(PO 4 ) 3 phosphates was determined. K 5 Zr(PO 4 ) 3 compound was characterized by the minimum thermal expansion at the near-zero anisotropy of Na 5 Zr(PO 4 ) 3 [ru

  14. The discrete and localized nature of the variable emission from active regions (United States)

    Arndt, Martina Belz; Habbal, Shadia Rifai; Karovska, Margarita


    Using data from the Extreme Ultraviolet (EUV) Spectroheliometer on Skylab, we study the empirical characteristics of the variable emission in active regions. These simultaneous multi-wavelength observations clearly confirm that active regions consist of a complex of loops at different temperatures. The variable emission from this complex has very well-defined properties that can be quantitatively summarized as follows: (1) It is localized predominantly around the footpoints where it occurs at discrete locations. (2) The strongest variability does not necessarily coincide with the most intense emission. (3) The fraction of the area of the footpoints, (delta n)/N, that exhibits variable emission, varies by +/- 15% as a function of time, at any of the wavelengths measured. It also varies very little from footpoint to footpoint. (4) This fractional variation is temperature dependent with a maximum around 10(exp 5) K. (5) The ratio of the intensity of the variable to the average background emission, (delta I)/(bar-I), also changes with temperature. In addition, we find that these distinctive characteristics persist even when flares occur within the active region.

  15. Instrumental neutron activation analysis as a tool for assessing the solubility of soil mineral matter in strong acid

    International Nuclear Information System (INIS)

    Steinnes, E.; Naeumann, R.


    Fifty samples of natural surface soils with high but variable organic matter content were analyzed for 13 elements (Na, Al, K, Sc, V, Cr, Mn, Fe, Co, Zn, Sr, Ba, La) by INAA. The same samples were analyzed for the 'total-recoverable' fraction of these elements by ICP-OES after decomposition with 7M HNO 3 , and the results are compared. The data are discussed separately for two groups of samples with organic matter contents of respectively >80% and Mn (77)>La (60)>Fe = Zn (53)>V (33)>Cr (29)>Sc (25)>Al = Ba (17)>Sr (13)>K (5)>Na (2). The results are in good agreement with corresponding literature data for mineral soils in the case of Al, K, Sc, V, Cr, Fe, and La. In the case of Na, Mn, Co, Zn, Sr, and Ba the present surface soils showed significantly higher 'total-recoverable' fractions than the previously studied subsoils. Possible reasons for these differences are discussed. INAA remains a convenient reference technique for determination of total concentrations with the rapidly increasing use of strong mineral acids in environmental studies of elements. (author)

  16. Using the area under the curve to reduce measurement error in predicting young adult blood pressure from childhood measures. (United States)

    Cook, Nancy R; Rosner, Bernard A; Chen, Wei; Srinivasan, Sathanur R; Berenson, Gerald S


    Tracking correlations of blood pressure, particularly childhood measures, may be attenuated by within-person variability. Combining multiple measurements can reduce this error substantially. The area under the curve (AUC) computed from longitudinal growth curve models can be used to improve the prediction of young adult blood pressure from childhood measures. Quadratic random-effects models over unequally spaced repeated measures were used to compute the area under the curve separately within the age periods 5-14 and 20-34 years in the Bogalusa Heart Study. This method adjusts for the uneven age distribution and captures the underlying or average blood pressure, leading to improved estimates of correlation and risk prediction. Tracking correlations were computed by race and gender, and were approximately 0.6 for systolic, 0.5-0.6 for K4 diastolic, and 0.4-0.6 for K5 diastolic blood pressure. The AUC can also be used to regress young adult blood pressure on childhood blood pressure and childhood and young adult body mass index (BMI). In these data, while childhood blood pressure and young adult BMI were generally directly predictive of young adult blood pressure, childhood BMI was negatively correlated with young adult blood pressure when childhood blood pressure was in the model. In addition, racial differences in young adult blood pressure were reduced, but not eliminated, after controlling for childhood blood pressure, childhood BMI, and young adult BMI, suggesting that other genetic or lifestyle factors contribute to this difference. 2004 John Wiley & Sons, Ltd.

  17. A Traffic Prediction Algorithm for Street Lighting Control Efficiency

    Directory of Open Access Journals (Sweden)

    POPA Valentin


    Full Text Available This paper presents the development of a traffic prediction algorithm that can be integrated in a street lighting monitoring and control system. The prediction algorithm must enable the reduction of energy costs and improve energy efficiency by decreasing the light intensity depending on the traffic level. The algorithm analyses and processes the information received at the command center based on the traffic level at different moments. The data is collected by means of the Doppler vehicle detection sensors integrated within the system. Thus, two methods are used for the implementation of the algorithm: a neural network and a k-NN (k-Nearest Neighbor prediction algorithm. For 500 training cycles, the mean square error of the neural network is 9.766 and for 500.000 training cycles the error amounts to 0.877. In case of the k-NN algorithm the error increases from 8.24 for k=5 to 12.27 for a number of 50 neighbors. In terms of a root means square error parameter, the use of a neural network ensures the highest performance level and can be integrated in a street lighting control system.

  18. Levels of use of an elementary school inquiry-based instructional innovation among a selected group of teacher participants in the Delaware Elementary Science Initiative (United States)

    Bouchelle, Henry Ellsworth Wirt, III

    Science education in Delaware's public elementary and middle schools has experienced much change in recent years as a result of the adoption of state standards and, in particular, the adoption by school districts of the Smithsonian/National Science Resources Council-sponsored inquiry-based instruction modules as part of the "Elementary Science Initiative." As part of this adoption process, each participating elementary teacher and middle school science teacher receives extensive training in the use of several discrete science kits. The trainings include reinforcement and development of content knowledge, in addition to the modeling of and practice with complementary pedagogy. One measure of the effectiveness of the science kit training process (and perhaps the Initiative itself) is the teachers' levels of use of the Initiative. The purpose of this study was to determine the participating teachers' use of the science kit innovation through the use of the Concerns-based Adoption Model Levels of Use Questionnaire. Eight K--5 elementary classroom teachers who had completed at least three science kit trainings participated. The results of this study indicate that on the Overall Level of Use Rating Scale, teachers who had completed training in at least three science kits generally scored at the Routine (IVA) level. All of the teachers, regardless of the wide range in the number of years of experience, had achieved the Mechanical Use level in Overall (III) LoU, and 6 of the 8 participants (75%) were operating at no less than the Refinement (IVA) Overall LoU level.

  19. Baseline assessment of doses and risk due to natural radionuclides in edible biota of Domiasiat, Meghalaya, India

    International Nuclear Information System (INIS)

    Kumar, N.; Chaturvedi, S.S.; Jha, S.K.


    Radiation dose-risk assessment was carried out for cereal species Brassica compestris var. dichotoma, Oryza sativa var. Shalum1, Zea mays, Lactuca indica, Cumunis sativum, and Clocasia esculanta due to naturally available radionuclides 40 K, 238 U and 232 Th in Domiasiat area. The activity in biota and corresponding soil was measured by precipitation method using NaI(TI) detector. Transfer factor (TF) was for Oryza spp. (1.00E-01- 40 K, 8.76E-05- 232 Th, and 9.11E-05- 238 U), for Brassica spp. (5.39E-01- 40 K, 8.17E-04- 232 Th and 2.96E-04- 238 U) and for Zea spp. (3.41E-01- 40 K, 5.84E-05- 232 Th, 8.87E-05- 238 U) etc., respectively. A detailed physio-morphological study of the biota and extensive investigation of ecosystem was carried out for assessment. The data was modeled using FASSET for dose estimation and obtained total dose was 1.58E-04 μGy h -1 in Oryza spp., 2.87E-04 μGy h -1 Brassica spp. and 6.90E-03 μGy h -1 in Zea spp. etc. The dose was compared with the UNSCEAR dataset for screening level dose for biota. Zea spp. was more susceptible for the chronic radiation exposure. (author)

  20. ATAD2 is an epigenetic reader of newly synthesized histone marks during DNA replication. (United States)

    Koo, Seong Joo; Fernández-Montalván, Amaury E; Badock, Volker; Ott, Christopher J; Holton, Simon J; von Ahsen, Oliver; Toedling, Joern; Vittori, Sarah; Bradner, James E; Gorjánácz, Mátyás


    ATAD2 (ATPase family AAA domain-containing protein 2) is a chromatin regulator harboring an AAA+ ATPase domain and a bromodomain, previously proposed to function as an oncogenic transcription co-factor. Here we suggest that ATAD2 is also required for DNA replication. ATAD2 is co-expressed with genes involved in DNA replication in various cancer types and predominantly expressed in S phase cells where it localized on nascent chromatin (replication sites). Our extensive biochemical and cellular analyses revealed that ATAD2 is recruited to replication sites through a direct interaction with di-acetylated histone H4 at K5 and K12, indicative of newly synthesized histones during replication-coupled chromatin reassembly. Similar to ATAD2-depletion, ectopic expression of ATAD2 mutants that are deficient in binding to these di-acetylation marks resulted in reduced DNA replication and impaired loading of PCNA onto chromatin, suggesting relevance of ATAD2 in DNA replication. Taken together, our data show a novel function of ATAD2 in cancer and for the first time identify a reader of newly synthesized histone di-acetylation-marks during replication.

  1. The Properties of the local Interstellar Medium and the Interaction of the Stellar Winds of epsilon Indi and lambda Andromedae with the Interstellar Environment (United States)

    Wood, Brian E.; Alexander, William R.; Linsky, Jeffrey L.


    We present new observations of the Ly alpha lines of Epsilon Indi (K5 5) and A Andromedae (G8 4-3 + ?) These data were obtained by the Goddard High Resolution Spectrograph (GHRS) on the Hubble Space Telescope. Analysis of the interstellar H 1 and D 1 absorption lines reveals that the velocities and temperatures inferred from the H 1 lines are inconsistent with the parameters inferred from the D 1 lines, unless the H 1 absorption is assumed to be produced by two absorption components. One absorption component is produced by interstellar material. For both lines of sight observed, the velocity of this component is consistent with the velocity predicted by the local flow vector. For the Epsilon Indi data, the large velocity separation between the stellar emission and the interstellar absorption allows us to measure the H 1 column density independent of the shape of the intrinsic stellar Ly alpha profile. This approach permits us to quote an accurate column density and to assess its uncertainty with far more confidence than in previous analyses, for which the errors were dominated by uncertainties in the assumed stellar profiles.

  2. Both a PKS and a PPTase are involved in melanin biosynthesis and regulation of Aureobasidium melanogenum XJ5-1 isolated from the Taklimakan desert. (United States)

    Jiang, Hong; Liu, Guang-Lei; Chi, Zhe; Wang, Jian-Ming; Zhang, Ly-Ly; Chi, Zhen-Ming


    A PKS1 gene responsible for the melanin biosynthesis and a NPG1 gene in Aureobasidium melanogenum XJ5-1 were cloned and characterized. An ORF of the PKS1 gene encoding a protein with 2165 amino acids contained 6495bp while an ORF of the NPG1 gene encoding a protein with 340 amino acids had 1076bp. After analysis of their promoters, it was found that expression of both the PKS1 gene and the NPG1 gene was repressed by nitrogen sources and glucose, respectively. The PKS deduced from the cloned gene consisted of one ketosynthase, one acyl transferase, two acyl carrier proteins, one thioesterase and one cyclase while the PPTase belonged to the family Sfp-type. After disruption of the PKS1 gene and the NPG1 gene, expression of the PKS1 gene and the NPG1 gene and the melanin biosynthesis in the disruptants K5 and DP107 disappeared and expression of the PKS1 gene in the disruptant DP107 was also negatively influenced. However, after the NPG1 gene was complemented in the disruptant DP107, the melanin biosynthesis in the complementary strain BP17 was restored and expression of the PKS1 gene and the NPG1 gene was greatly enhanced, suggesting that the PKS was indeed activated and regulated by the PPTase and expression of the PKS1 gene and the NPG1 gene had a coordinate regulation. Copyright © 2016 Elsevier B.V. All rights reserved.

  3. Characterization of elasticity and hydration of composite hydrogel based on collagen-iota carrageenan as a corneal tissue engineering (United States)

    Rinawati, M.; Triastuti, J.; Pursetyo, K. T.


    The cornea is a refractive element of the eye that serves to continue the stimulation of light into the eye it has a clear, transparent, elastic and relatively thick tissue. Factors caused corneal blindness, are dystrophy, keratoconus, corneal scaring. Hydrogels can be made from polysaccharide derivatives that have gelation properties such as iota carrageenan. Therefore, it is a need to develop composite hydrogel based collagen-iota carragenan as an engineeried corneal tissue with high elasticity and hydration properties. Collagen hydrogel has a maximum water content an has equlibrium up to 40 %, less than the human cornea, 81 % and under normal hydration conditions, the human cornea can transmit 87 % of visible light. In addition, the refractive index on the surface of the cornea with air is 1.375-1.380. Based on this study, it is necessary to conduct research on the development and composition of hydrogel composite collagen-iota carrageen hydrogen based on. The best result was K5 (5:5) treatment, which has the equilibrium water content of 87.07 % and viscosity of 10.7346 Pa.s.

  4. High-throughput identification of chemical inhibitors of E. coli Group 2 capsule biogenesis as anti-virulence agents.

    Directory of Open Access Journals (Sweden)

    Carlos C Goller

    Full Text Available Rising antibiotic resistance among Escherichia coli, the leading cause of urinary tract infections (UTIs, has placed a new focus on molecular pathogenesis studies, aiming to identify new therapeutic targets. Anti-virulence agents are attractive as chemotherapeutics to attenuate an organism during disease but not necessarily during benign commensalism, thus decreasing the stress on beneficial microbial communities and lessening the emergence of resistance. We and others have demonstrated that the K antigen capsule of E. coli is a preeminent virulence determinant during UTI and more invasive diseases. Components of assembly and export are highly conserved among the major K antigen capsular types associated with UTI-causing E. coli and are distinct from the capsule biogenesis machinery of many commensal E. coli, making these attractive therapeutic targets. We conducted a screen for anti-capsular small molecules and identified an agent designated "C7" that blocks the production of K1 and K5 capsules, unrelated polysaccharide types among the Group 2-3 capsules. Herein lies proof-of-concept that this screen may be implemented with larger chemical libraries to identify second-generation small-molecule inhibitors of capsule biogenesis. These inhibitors will lead to a better understanding of capsule biogenesis and may represent a new class of therapeutics.

  5. Iris Recognition Using Feature Extraction of Box Counting Fractal Dimension (United States)

    Khotimah, C.; Juniati, D.


    Biometrics is a science that is now growing rapidly. Iris recognition is a biometric modality which captures a photo of the eye pattern. The markings of the iris are distinctive that it has been proposed to use as a means of identification, instead of fingerprints. Iris recognition was chosen for identification in this research because every human has a special feature that each individual is different and the iris is protected by the cornea so that it will have a fixed shape. This iris recognition consists of three step: pre-processing of data, feature extraction, and feature matching. Hough transformation is used in the process of pre-processing to locate the iris area and Daugman’s rubber sheet model to normalize the iris data set into rectangular blocks. To find the characteristics of the iris, it was used box counting method to get the fractal dimension value of the iris. Tests carried out by used k-fold cross method with k = 5. In each test used 10 different grade K of K-Nearest Neighbor (KNN). The result of iris recognition was obtained with the best accuracy was 92,63 % for K = 3 value on K-Nearest Neighbor (KNN) method.

  6. Kinetic mechanism and isotope effects of Pseudomonas cepacia 3-hydroxybenzoate-t-hydroxylase

    International Nuclear Information System (INIS)

    Wang, L.H.; Yu, Y.; Hamzah, R.Y.; Tu, S.C.


    The kinetic mechanism of Pseudomonas cepacia 3-hydroxybenzoate-6-hydroxylase has been delineated. Double reciprocal plots of initial rate versus m-hydroxybenzoate concentration at a constant level of oxygen and several fixed concentrations of NADH yielded a set of converging lines. Similar reciprocal plots of velocity versus NADH concentration at a constant oxygen level and several fixed m-hydroxybenzoate concentrations also showed converging lines. In contrast, double reciprocal plots of initial rate versus NADH concentration at a fixed m-hydroxybenzoate level and several oxygen concentrations showed a series of parallel lines. Parallel lines were also obtained from double reciprocal plots of initial rate versus m-hydroxybenzoate concentration at a fixed NADH level and varying oxygen concentrations. These results suggest a sequential binding of m-hydroxybenzoate and NADH by the hydroxylase. The enzyme-bound FAD is reduced and NAD is released. The reduced enzyme subsequently reacts with oxygen leading to the formation of other products. This hydroxylase exhibited a primary isotope effect of /sup D/V = 3.5 for (4R)-[4- 2 H] NADH but no isotope effect was observed with (4S)-[4- 2 H]NADH. An isotope effect of /sup T/V/K = 5.0 was also observed using (4R)-[4- 3 H]NADH. This tritium isotope effect was apparently independent of m-hydroxybenzoate concentration

  7. Effects of a mixture of tetracyclines to Lemna gibba and Myriophyllum sibiricum evaluated in aquatic microcosms

    International Nuclear Information System (INIS)

    Brain, Richard A.; Wilson, Christian J.; Johnson, David J.; Sanderson, Hans; Bestari, Ketut; Hanson, Mark L.; Sibley, Paul K.; Solomon, Keith R.


    The impact of a mixture of oxytetracycline, chlortetracycline, tetracycline and doxycycline on Myriophyllum sibiricum and Lemna gibba was investigated using fifteen 12,000-L microcosms (k = 5, n = 3). Significant concentration-response relationships were only found for M. sibiricum, where dry mass was 69, 47, 30, and 7% of controls at respective treatment concentrations of 0.080, 0.218, 0.668, and 2.289 μmol/L. Somatic endpoints were strongly and negatively correlated with percent light transmission, except plant length, which was positively correlated. Treated microcosms experienced a reduction in the percent of surface irradiance penetrating the water column as high as 99.8% at a depth of 70 cm, relative to controls. Position relative to the water column was likely responsible for the differential effects observed between floating (L. gibba) and submerged (M. sibiricum) species of macrophytes. A hazard quotient assessment of the lowest EC 10 value indicated significant risk, exceeding the critical HQ value, but not the lowest EC 25 value. - Mixtures of tetracycline antibiotics pose a risk to submerged but not floating aquatic plants


    International Nuclear Information System (INIS)

    Geißler, Kerstin; Metchev, Stanimir A.; Pham, Alfonse; Larkin, James E.; McElwain, Michael; Hillenbrand, Lynne A.


    We announce the identification of a proper-motion companion to the star H II 1348, a K5 V member of the Pleiades open cluster. The existence of a faint point source 1.''1 away from H II 1348 was previously known from adaptive optics imaging by Bouvier et al. However, because of a high likelihood of background star contamination and in the absence of follow-up astrometry, Bouvier et al. tentatively concluded that the candidate companion was not physically associated with H II 1348. We establish the proper-motion association of the pair from adaptive optics imaging with the Palomar 5 m telescope. Adaptive optics spectroscopy with the integral field spectrograph OSIRIS on the Keck 10 m telescope reveals that the companion has a spectral type of M8 ± 1. According to substellar evolution models, the M8 spectral type resides within the substellar mass regime at the age of the Pleiades. The primary itself is a known double-lined spectroscopic binary, which makes the resolved companion, H II 1348B, the least massive and widest component of this hierarchical triple system and the first substellar companion to a stellar primary in the Pleiades.

  9. Verification of the Sentinel-4 focal plane subsystem (United States)

    Williges, Christian; Uhlig, Mathias; Hilbert, Stefan; Rossmann, Hannes; Buchwinkler, Kevin; Babben, Steffen; Sebastian, Ilse; Hohn, Rüdiger; Reulke, Ralf


    The Sentinel-4 payload is a multi-spectral camera system, designed to monitor atmospheric conditions over Europe from a geostationary orbit. The German Aerospace Center, DLR Berlin, conducted the verification campaign of the Focal Plane Subsystem (FPS) during the second half of 2016. The FPS consists, of two Focal Plane Assemblies (FPAs), two Front End Electronics (FEEs), one Front End Support Electronic (FSE) and one Instrument Control Unit (ICU). The FPAs are designed for two spectral ranges: UV-VIS (305 nm - 500 nm) and NIR (750 nm - 775 nm). In this publication, we will present in detail the set-up of the verification campaign of the Sentinel-4 Qualification Model (QM). This set up will also be used for the upcoming Flight Model (FM) verification, planned for early 2018. The FPAs have to be operated at 215 K +/- 5 K, making it necessary to exploit a thermal vacuum chamber (TVC) for the test accomplishment. The test campaign consists mainly of radiometric tests. This publication focuses on the challenge to remotely illuminate both Sentinel-4 detectors as well as a reference detector homogeneously over a distance of approximately 1 m from outside the TVC. Selected test analyses and results will be presented.

  10. A SNAPshot of the FUV (1320 - 1460 A) Spectrum of Lambda Vel (K4Ib-II) (United States)

    Carpenter, Kenneth G.


    The FUV spectrum (l330-1460A) of the K4Ib-II supergiant Lambda Vel was observed with the Cosmic Origins Spectrograph (COS) on HST, as part of the Ayres and Redfield Cycle 17 SNAP program "SNAPing Coronal Iron". This spectrum covers a region not previously seen in Lambda Vel at high resolution and reveals a rich emission-line spectrum superposed on a bright continuum, with contributions from a variety of atomic and molecular sources. Evidence of the stellar wind is seen in the P Cygni profiles of selected lines and the results of fluorescence processes are visible throughout the region. The spectrum has remarkable similarities to that of Alpha Boo (K1.5 III), but significant differences as well, including substantial FUV continuum emission, reminiscent of the M2 lab supergiant Alpha Ori, but minus the CO fundamental absorption bands seen in the latter star. However, fluoresced CO emission is present and strong, as in the K-giant stars Alpha Boo and Alpha Tau (K5 III). We present the details of this spectrum, in comparison to stars of similar temperature or luminosity and discuss the implications for the structure of and the radiative processes active in, the outer atmospheres of these stars.

  11. Finding New Math Identities by Computer (United States)

    Bailey, David H.; Chancellor, Marisa K. (Technical Monitor)


    Recently a number of interesting new mathematical identities have been discovered by means of numerical searches on high performance computers, using some newly discovered algorithms. These include the following: pi = ((sup oo)(sub k=0))(Sigma) (1 / 16) (sup k) ((4 / 8k+1) - (2 / 8k+4) - (1 / 8k+5) - (1 / 8k+6)) and ((17 pi(exp 4)) / 360) = ((sup oo)(sub k=1))(Sigma) (1 + (1/2) + (1/3) + ... + (1/k))(exp 2) k(exp -2), zeta(3, 1, 3, 1, ..., 3, 1) = (2 pi(exp 4m) / (4m+2)! where m = number of (3,1) pairs. and where zeta(n1,n2,...,nr) = (sub k1 (is greater than) k2 (is greater than) ... (is greater than) kr)(Sigma) (1 / (k1 (sup n1) k2 (sup n2) ... kr (sup nr). The first identity is remarkable in that it permits one to compute the n-th binary or hexadecimal digit of pu directly, without computing any of the previous digits, and without using multiple precision arithmetic. Recently the ten billionth hexadecimal digit of pi was computed using this formula. The third identity has connections to quantum field theory. (The first and second of these been formally established; the third is affirmed by numerical evidence only.) The background and results of this work will be described, including an overview of the algorithms and computer techniques used in these studies.

  12. Link lengths and their growth powers

    International Nuclear Information System (INIS)

    Huh, Youngsik; No, Sungjong; Oh, Seungsang; Rawdon, Eric J


    For a certain infinite family F of knots or links, we study the growth power ratios of their stick number, lattice stick number, minimum lattice length and minimum ropelength compared with their minimum crossing number c(K) for every K∈F. It is known that the stick number and lattice stick number grow between the (1/2) and linear power of the crossing number, and minimum lattice length and minimum ropelength grow with at least the (3/4) power of crossing number (which is called the four-thirds power law). Furthermore, the minimal lattice length and minimum ropelength grow at most as O (c(K)[ln(c(K))] 5 ), but it is unknown whether any family exhibits superlinear growth. For any real number r between (1/2) and 1, we give an infinite family of non-splittable prime links in which the stick number and lattice stick number grow exactly as the rth power of crossing number. Furthermore for any real number r between (3/4) and 1, we give another infinite family of non-splittable prime links in which the minimum lattice length and minimum ropelength grow exactly as the rth power of crossing number. (paper)

  13. Sound Shell Model for Acoustic Gravitational Wave Production at a First-Order Phase Transition in the Early Universe (United States)

    Hindmarsh, Mark


    A model for the acoustic production of gravitational waves at a first-order phase transition is presented. The source of gravitational radiation is the sound waves generated by the explosive growth of bubbles of the stable phase. The model assumes that the sound waves are linear and that their power spectrum is determined by the characteristic form of the sound shell around the expanding bubble. The predicted power spectrum has two length scales, the average bubble separation and the sound shell width when the bubbles collide. The peak of the power spectrum is at wave numbers set by the sound shell width. For a higher wave number k , the power spectrum decreases to k-3. At wave numbers below the inverse bubble separation, the power spectrum goes to k5. For bubble wall speeds near the speed of sound where these two length scales are distinguished, there is an intermediate k1 power law. The detailed dependence of the power spectrum on the wall speed and the other parameters of the phase transition raises the possibility of their constraint or measurement at a future space-based gravitational wave observatory such as LISA.

  14. Combined Approach for the Structural Characterization of Alkali Fluoroscandates: Solid-State NMR, Powder X-ray Diffraction, and Density Functional Theory Calculations. (United States)

    Rakhmatullin, Aydar; Polovov, Ilya B; Maltsev, Dmitry; Allix, Mathieu; Volkovich, Vladimir; Chukin, Andrey V; Boča, Miroslav; Bessada, Catherine


    The structures of several fluoroscandate compounds are presented here using a characterization approach combining powder X-ray diffraction and solid-state NMR. The structure of K 5 Sc 3 F 14 was fully determined from Rietveld refinement performed on powder X-ray diffraction data. Moreover, the local structures of NaScF 4 , Li 3 ScF 6 , KSc 2 F 7 , and Na 3 ScF 6 compounds were studied in detail from solid-state 19 F and 45 Sc NMR experiments. The 45 Sc chemical shift ranges for six- and seven-coordinated scandium environments were defined. The 19 F chemical shift ranges for bridging and terminal fluorine atoms were also determined. First-principles calculations of the 19 F and 45 Sc NMR parameters were carried out using plane-wave basis sets and periodic boundary conditions (CASTEP), and the results were compared with the experimental data. A good agreement between the calculated shielding constants and experimental chemical shifts was obtained. This demonstrates the good potential of computational methods in spectroscopic assignments of solid-state 45 Sc NMR spectroscopy.

  15. Vapor-liquid critical surface of ternary difluoromethane + pentafluoroethane + 1,1,1,2-tetrafluoroethane (R-32/125/134a) mixtures

    Energy Technology Data Exchange (ETDEWEB)

    Higashi, Y.


    The plane of vapor-liquid criticality for ternary refrigerant mixtures of difluoromethane (R-32) + pentafluoroethane (R-125) + 1,1,1,2-tetrafluoroethane (R-134a) was determined from data on the vapor-liquid coexistence curve near the mixture critical points. The composition (mass percentage) of the mixtures studied were 23% R-32 + 25% R-125 + 52% R-134a (R-407C). 25% R-32 + 15% R-125 + 60% R-134a (R-407E), and 20% R-32 + 40% R-125 + 40% R-134a (R-407A). The critical temperature of each mixture was determined by observation of the disappearance of the meniscus. The critical density of each mixture was determined on the basis of meniscus disappearance level and the intensity of the critical opalescence. The uncertainties of the temperature, density, and composition measurements are estimated as {+-}10mK, {+-}5kg{center_dot}m{sup {minus}3}, and {+-}0.05%, respectively. In addition, predictive methods for the critical parameters of R-32/125/134a mixtures are discussed.

  16. Quantitative Literacy and the Common Core State Standards in Mathematics

    Directory of Open Access Journals (Sweden)

    Bernard L. Madison


    Full Text Available How supportive of quantitative literacy (QL are the Common Core State Standards in Mathematics (CCSSM? The answer is tentative and conditional. There are some QL-supportive features including a strong probability and statistics strand in grade 6 through high school; a measurements and data strand in K-5; ratio and proportional reasoning standards in grades 6 and 7; and a comprehensive and coherent approach to algebraic reasoning and logical argument. However, the standards are weak in supporting reasoning and interpretation, and there are indications that the applications in CCSSM – mostly unspecified – will not include many QL contextual situations. Early indicators of assessment items follow a similar path. Except for statistics, most of the high school standards are aimed at development of algebra and precalculus topics, and there will likely be little room for more sophisticated applications of the QL-friendly mathematics of grades 6-8. The experience with CCSSM is limited at this point, leaving several crucial results uncertain, including assessments, emphases on statistics, and kinds of modeling and other applications.

  17. Sound Shell Model for Acoustic Gravitational Wave Production at a First-Order Phase Transition in the Early Universe. (United States)

    Hindmarsh, Mark


    A model for the acoustic production of gravitational waves at a first-order phase transition is presented. The source of gravitational radiation is the sound waves generated by the explosive growth of bubbles of the stable phase. The model assumes that the sound waves are linear and that their power spectrum is determined by the characteristic form of the sound shell around the expanding bubble. The predicted power spectrum has two length scales, the average bubble separation and the sound shell width when the bubbles collide. The peak of the power spectrum is at wave numbers set by the sound shell width. For a higher wave number k, the power spectrum decreases to k^{-3}. At wave numbers below the inverse bubble separation, the power spectrum goes to k^{5}. For bubble wall speeds near the speed of sound where these two length scales are distinguished, there is an intermediate k^{1} power law. The detailed dependence of the power spectrum on the wall speed and the other parameters of the phase transition raises the possibility of their constraint or measurement at a future space-based gravitational wave observatory such as LISA.

  18. Advanced Spectral Library (ASTRAL): Atomic Fluorescence in Cool, Evolved Stars (United States)

    Carpenter, Ken G.; Nielsen, Krister E.; Kober, Gladys V.; Rau, Gioia


    The "Advanced Spectral Library (ASTRAL) Project: Cool Stars" (PI = T. Ayres) collected a definitive set of representative, high-resolution (R~46,000 in the FUV up to ~1700 Å, R~30,000 for 1700-2150 Å, and R~114,000 >2150 Å) and high signal/noise (S/N>100) UV spectra of eight F-M evolved cool stars. These extremely high-quality STIS UV echelle spectra are available from the HST archive and from the Univ. of Colorado ( and will enable investigations of a broad range of problems -- stellar, interstellar, and beyond -- for many years. In this paper, we extend our study of the very rich emission-line spectra of the four evolved K-M stars in the sample, Beta Gem (K0 IIIb), Gamma Dra (K5 III), Gamma Cru (M3.4 III), and Alpha Ori (M2 Iab), to study the atomic fluorescence processes operating in their outer atmospheres. We summarize the pumping transitions and fluorescent line products known on the basis of previous work (e.g. Carpenter 1988, etc.) and newly identified in our current, on-going analysis of these extraordinary ASTRAL STIS spectra.

  19. A new photometric metal abundance and luminosity calibration for field G and K giants

    International Nuclear Information System (INIS)

    Jennens, P.A.; Helfer, H.L.


    Photometry of 260 G and K giants, using a fast broad-intermediate band photometric system (UBViyz system) is used to calibrate chemical composition, Fe/H], luminosity, Mv and colour excess, E(B-V). A single S-20 surface photomultiplier is used. The UBVi photometry is transformed to be on the Johnson UBVRI system. Calibrations applicable to the ranges 0.40< R-I<0.65 (G2-K3), 0.65< R-I<0.90 (K3-K5) are given. A photometric luminosity index, Mv(yz), is derived for which rms errors are +-1 mag. Several indices are calibrated for chemical composition, [Fe/H], and typical rms errors of +-0.15 in [Fe/H] are obtained for stars of known colour excess, E(B-V). For stars of unknown colour excess, E(B-V) is determined with an rms error of +-0.06 and [Fe/H] with an rms error of approximately +-0.4. For stars with Mv-1, the errors are larger. (author)


    Energy Technology Data Exchange (ETDEWEB)

    Santerne, A.; Barros, S. C. C.; Mena, E. Delgado; Montalto, M.; Sousa, S. G.; Adibekyan, V. [Instituto de Astrofísica e Ciências do Espaço, Universidade do Porto, CAUP, Rua das Estrelas, 4150-762 Porto (Portugal); Hébrard, G. [Institut d’Astrophysique de Paris, UMR7095 CNRS, Université Pierre and Marie Curie, 98bis boulevard Arago, F-75014 Paris (France); Lillo-Box, J.; Barrado, D. [Departamento de Astrofsica, Centro de Astrobiologa (CSIC-INTA), ESAC campus E-28692 Villanueva de la Caada (Madrid) (Spain); Armstrong, D. J.; Pollacco, D.; Osborn, H. P. [Department of Physics, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL (United Kingdom); Demangeon, O.; Deleuil, M. [Aix Marseille Université, CNRS, Laboratoire d’Astrophysique de Marseille UMR 7326, F-13388, Marseille (France); Debackere, A.; Arlic, G.; Barthe, G. [European Pro/Am Network of Exoplanetary Transit Observers (France); Abe, L. [Laboratoire Lagrange, UMR7239, Université de Nice Sophia-Antipolis, CNRS, Observatoire de la Cote d’Azur, F-06300 Nice (France); Almenara, J.-M. [Université Grenoble Alpes, IPAG, F-38000 Grenoble (France); André, P., E-mail: [Observatoire de Belesta-en-Lauragais-Assoc. Astronomie Adagio 30 Route de Revel, F-31450 Varennes (France); and others


    In the present paper we report the discovery of a new hot Jupiter, K2-29 b, first detected by the Super-WASP observatory and then by the K2 space mission during its campaign 4. The planet has a period of 3.25 days, a mass of 0.73 ± 0.04 M {sub ♃}, and a radius of 1.19 ± 0.02 R {sub ♃}. The host star is a relatively bright ( V = 12.5) G7 dwarf with a nearby K5V companion. Based on stellar rotation and the abundance of lithium, we find that the system might be as young as ∼450 Myr. The observation of the Rossiter–McLaughlin effect shows that the planet is aligned with respect to the stellar spin. Given the deep transit (20 mmag), the magnitude of the star and the presence of a nearby stellar companion, the planet is a good target for both space- and ground-based transmission spectroscopy, in particular in the near-infrared where both stars are relatively bright.

  1. The pro-angiogenic properties of multi-functional bioactive glass composite scaffolds

    KAUST Repository

    Gerhardt, Lutz Christian


    The angiogenic properties of micron-sized (m-BG) and nano-sized (n-BG) bioactive glass (BG) filled poly(D,L lactide) (PDLLA) composites were investigated. On the basis of cell culture work investigating the secretion of vascular endothelial growth factor (VEGF) by human fibroblasts in contact with composite films (0, 5, 10, 20 wt %), porous 3D composite scaffolds, optimised with respect to the BG filler content capable of inducing angiogenic response, were produced. The in vivo vascularisation of the scaffolds was studied in a rat animal model and quantified using stereological analyses. The prepared scaffolds had high porosities (81-93%), permeability (k = 5.4-8.6 × 10-9 m2) and compressive strength values (0.4-1.6 MPa) all in the range of trabecular bone. On composite films containing 20 wt % m-BG or n-BG, human fibroblasts produced 5 times higher VEGF than on pure PDLLA films. After 8 weeks of implantation, m-BG and n-BG containing scaffolds were well-infiltrated with newly formed tissue and demonstrated higher vascularisation and percentage blood vessel to tissue (11.6-15.1%) than PDLLA scaffolds (8.5%). This work thus shows potential for the regeneration of hard-soft tissue defects and increased bone formation arising from enhanced vascularisation of the construct. © 2011 Elsevier Ltd.

  2. A new approach to very short term wind speed prediction using k-nearest neighbor classification

    International Nuclear Information System (INIS)

    Yesilbudak, Mehmet; Sagiroglu, Seref; Colak, Ilhami


    Highlights: ► Wind speed parameter was predicted in an n-tupled inputs using k-NN classification. ► The effects of input parameters, nearest neighbors and distance metrics were analyzed. ► Many useful and reasonable inferences were uncovered using the developed model. - Abstract: Wind energy is an inexhaustible energy source and wind power production has been growing rapidly in recent years. However, wind power has a non-schedulable nature due to wind speed variations. Hence, wind speed prediction is an indispensable requirement for power system operators. This paper predicts wind speed parameter in an n-tupled inputs using k-nearest neighbor (k-NN) classification and analyzes the effects of input parameters, nearest neighbors and distance metrics on wind speed prediction. The k-NN classification model was developed using the object oriented programming techniques and includes Manhattan and Minkowski distance metrics except from Euclidean distance metric on the contrary of literature. The k-NN classification model which uses wind direction, air temperature, atmospheric pressure and relative humidity parameters in a 4-tupled space achieved the best wind speed prediction for k = 5 in the Manhattan distance metric. Differently, the k-NN classification model which uses wind direction, air temperature and atmospheric pressure parameters in a 3-tupled inputs gave the worst wind speed prediction for k = 1 in the Minkowski distance metric

  3. The structures of bacteriophages K1E and K1-5 explain processive degradation of polysaccharide capsules and evolution of new host specificities. (United States)

    Leiman, Petr G; Battisti, Anthony J; Bowman, Valorie D; Stummeyer, Katharina; Mühlenhoff, Martina; Gerardy-Schahn, Rita; Scholl, Dean; Molineux, Ian J


    External polysaccharides of many pathogenic bacteria form capsules protecting the bacteria from the animal immune system and phage infection. However, some bacteriophages can digest these capsules using glycosidases displayed on the phage particle. We have utilized cryo-electron microscopy to determine the structures of phages K1E and K1-5 and thereby establish the mechanism by which these phages attain and switch their host specificity. Using a specific glycosidase, both phages penetrate the capsule and infect the neuroinvasive human pathogen Escherichia coli K1. In addition to the K1-specific glycosidase, each K1-5 particle carries a second enzyme that allows it to infect E. coli K5, whose capsule is chemically different from that of K1. The enzymes are organized into a multiprotein complex attached via an adapter protein to the virus portal vertex, through which the DNA is ejected during infection. The structure of the complex suggests a mechanism for the apparent processivity of degradation that occurs as the phage drills through the polysaccharide capsule. The enzymes recognize the adapter protein by a conserved N-terminal sequence, providing a mechanism for phages to acquire different enzymes and thus to evolve new host specificities.

  4. Influence of an Intensive, Field-Based Life Science Course on Preservice Teachers' Self-Efficacy for Environmental Science Teaching (United States)

    Trauth-Nare, Amy


    Personal and professional experiences influence teachers' perceptions of their ability to implement environmental science curricula and to positively impact students' learning. The purpose of this study was twofold: to determine what influence, if any, an intensive field-based life science course and service learning had on preservice teachers' self-efficacy for teaching about the environment and to determine which aspects of the combined field-based course/service learning preservice teachers perceived as effective for enhancing their self-efficacy. Data were collected from class documents and written teaching reflections of 38 middle-level preservice teachers. Some participants ( n = 18) also completed the Environmental Education Efficacy Belief Instrument at the beginning and end of the semester. Both qualitative and quantitative data analyses indicated a significant increase in PSTs' personal efficacies for environmental teaching, t(17) = 4.50, p = .000, d = 1.30, 95 % CI (.33, .90), but not outcome expectancy, t(17) = 1.15, p = .268, d = .220, 95 % CI (-.06, .20). Preservice teachers reported three aspects of the course as important for enhancing their self-efficacies: learning about ecological concepts through place-based issues, service learning with K-5 students and EE curriculum development. Data from this study extend prior work by indicating that practical experiences with students were not the sole factor in shaping PSTs' self-efficacy; learning ecological concepts and theories in field-based activities grounded in the local landscape also influenced PSTs' self-efficacy.

  5. Determination of Fatty Acid Metabolism with Dynamic [11C]Palmitate Positron Emission Tomography of Mouse Heart In Vivo

    Directory of Open Access Journals (Sweden)

    Yinlin Li


    Full Text Available The goal of this study was to establish a quantitative method for measuring fatty acid (FA metabolism with partial volume (PV and spill-over (SP corrections using dynamic [11C]palmitate positron emission tomographic (PET images of mouse heart in vivo. Twenty-minute dynamic [11C]palmitate PET scans of four 18- to 20-week-old male C57BL/6 mice under isoflurane anesthesia were performed using a Focus F-120 PET scanner. A model-corrected blood input function, by which the input function with SP and PV corrections and the metabolic rate constants (k1–k5 are simultaneously estimated from the dynamic [11C]palmitate PET images of mouse hearts in a four-compartment tracer kinetic model, was used to determine rates of myocardial fatty acid oxidation (MFAO, myocardial FA esterification, myocardial FA use, and myocardial FA uptake. The MFAO thus measured in C57BL/6 mice was 375.03 ± 43.83 nmol/min/g. This compares well to the MFAO measured in perfused working C57BL/6 mouse hearts ex vivo of about 350 nmol/g/min and 400 nmol/min/g. FA metabolism was measured for the first time in mouse heart in vivo using dynamic [11C]palmitate PET in a four-compartment tracer kinetic model. MFAO obtained with this model was validated by results previously obtained with mouse hearts ex vivo.

  6. The analyze of lung’s GSH number in rats exposed by cigarette smoke and inducted by rambutan peel extract (United States)



    The cigarette smoke is one of the pollutants in human and environment. It contains free radical compounds which cause oxidative stress. In the oxidative stress condition, the free radical causing peroxidation of cell membrane lipid as well as damages the cell membrane. One of the biomarkers of oxidative stress happens the number of GSH. The purpose of this study was to analyze the amount of rat's GSH which exposed by cigarette smoke as well as inducted by rambutan pell extract. This study applied to 25 male rats of Wistar which divided into five groups; K1 (control), K2 (negative), K3, K4, and K5 were the treatment groups of rambutan peel extract with various dosage; 3, 6, 12 mg/200 gramBB and cigarette smoke exposure along 30 days. The number of GSH measured by the DTNB of lung tissue. To know the difference of GSH number of each group did the data analysis with one way ANOVA test and LSD advance test. The result of statistic analysis showed that there was a significant difference between the control group and treatment group. The conclusion of this study was the rambutan peel extract with 3 mg/200 gramBB dosage could increase the number of lung's GSH of rats exposed to cigarette smoke.

  7. Taurocholate Deconjugation and Cholesterol Binding by Indigenous Dadih Lactic Acid Bacteria

    Directory of Open Access Journals (Sweden)



    Full Text Available High serum cholesterol levels have been associated with an increased risk for human coronary heart disease. Lowering of serum cholesterol has been suggested to prevent the heart disease. To reduce serum cholesterol levels one may consumed diet supplementat of fermented dairy product such as dadih. Lactic acid bacteria present in dadih may alter serum cholesterol by directly bind to dietary cholesterol and/or deconjugation of bile salts. Acid and bile tolerance, deconjugation of sodium taurocholate, and the cholesterol-binding ability of lactic acid bacteria from dadih were examined. Among ten dadih lactic acid bacteria tested, six strains namely I-11, I-2775, K-5, I-6257, IS-7257, and B-4 could bind cholesterol and deconjugate sodium taurocholate. However, the last four strains were very sensitive to bile. Therefore, Lactobacillus fermentum I-11 and Leuconostoc lactis subsp. lactis I-2775 those were tolerant to acid and oxgall (bile and deconjugated sodium taurocholate and bound cholesterol could be recommended as probiotic to prevent coronary heart disease.

  8. Enhanced Thermal Conductivity of Polyimide Composites Filled with Modified h-BN and Nanodiamond Hybrid Filler. (United States)

    Yang, Xi; Yu, Xiaoyan; Naito, Kimiyoshi; Ding, Huili; Qu, Xiongwei; Zhang, Qingxin


    A new thermally conductive and electrically insulative polyimide were prepared by filling different amounts of hexagonal boron nitride (h-BN) particles, and the thermal conductivity of Polyimide (PI) composites were improved with the increasing h-BN content. Based on this, two methods were applied to improve thermal conductivity furtherly at limited filler loading in this paper. One is modifying the h-BN to improve interface interaction, another is fabricating a nano-micro hybrid filler with 2-D h-BN and 0-D nano-scale nanodiamond (ND) to build more effective conductive network. Both surface modification and hybrid system have a positive effect on thermal conductivity. The composites introducing 40 wt% hybrid filler (the weight ratio of ND/modified BN was 1/10) showed the highest thermal conductivity, being up to 0.98 W/(m K) (5.2 times that of PI). In addition, the composites exhibits excellent electrical insulation, thermal stability properties etc.

  9. Phylogenetic and Diversity Analysis of Dactylis glomerata Subspecies Using SSR and IT-ISJ Markers. (United States)

    Yan, Defei; Zhao, Xinxin; Cheng, Yajuan; Ma, Xiao; Huang, Linkai; Zhang, Xinquan


    The genus Dactylis , an important forage crop, has a wide geographical distribution in temperate regions. While this genus is thought to include a single species, Dactylis glomerata , this species encompasses many subspecies whose relationships have not been fully characterized. In this study, the genetic diversity and phylogenetic relationships of nine representative Dactylis subspecies were examined using SSR and IT-ISJ markers. In total, 21 pairs of SSR primers and 15 pairs of IT-ISJ primers were used to amplify 295 polymorphic bands with polymorphic rates of 100%. The average polymorphic information contents (PICs) of SSR and IT-ISJ markers were 0.909 and 0.780, respectively. The combined data of the two markers indicated a high level of genetic diversity among the nine D. glomerata subspecies, with a Nei's gene diversity index value of 0.283 and Shannon's diversity of 0.448. Preliminarily phylogenetic analysis results revealed that the 20 accessions could be divided into three groups (A, B, C). Furthermore, they could be divided into five clusters, which is similar to the structure analysis with K = 5. Phylogenetic placement in these three groups may be related to the distribution ranges and the climate types of the subspecies in each group. Group A contained eight accessions of four subspecies, originating from the west Mediterranean, while Group B contained seven accessions of three subspecies, originating from the east Mediterranean.

  10. Crossover distribution and frequency are regulated by him-5 in Caenorhabditis elegans. (United States)

    Meneely, Philip M; McGovern, Olivia L; Heinis, Frazer I; Yanowitz, Judith L


    Mutations in the him-5 gene in Caenorhabditis elegans strongly reduce the frequency of crossovers on the X chromosome, with lesser effects on the autosomes. him-5 mutants also show a change in crossover distribution on both the X and autosomes. These phenotypes are accompanied by a delayed entry into pachytene and premature desynapsis of the X chromosome. The nondisjunction, progression defects and desynapsis can be rescued by an exogenous source of double strand breaks (DSBs), indicating that the role of HIM-5 is to promote the formation of meiotic DSBs. Molecular cloning of the gene shows that the inferred HIM-5 product is a highly basic protein of 252 amino acids with no clear orthologs in other species, including other Caenorhabditis species. Although him-5 mutants are defective in segregation of the X chromosome, HIM-5 protein localizes preferentially to the autosomes. The mutant phenotypes and localization of him-5 are similar but not identical to the results seen with xnd-1, although unlike xnd-1, him-5 has no apparent effect on the acetylation of histone H2A on lysine 5 (H2AacK5). The localization of HIM-5 to the autosomes depends on the activities of both xnd-1 and him-17 allowing us to begin to establish pathways for the control of crossover distribution and frequency.

  11. Atomic data and spectral line intensities for Ne III

    CERN Document Server

    Bhatia, A K; Landi, E


    Electron impact collision strengths, energy levels, oscillator strengths and spontaneous radiative decay rates are calculated for Ne III. The configurations used are 2s sup 2 2p sup 4 , 2s2p sup 5 , 2s sup 2 2p sup 3 3s, and 2s sup 2 2p sup 3 3d giving rise to 57 fine-structure levels in intermediate coupling. Collision strengths are calculated at five incident energies, 5, 15, 25, 35, and 45 Ry. Excitation rate coefficients are calculated by assuming a Maxwellian electron velocity distribution at an electron temperature of logT sub e (K)=5.0, corresponding to maximum abundance of Ne III. Using the excitation rate coefficients and the radiative transition rates, statistical equilibrium equations for level populations are solved at electron densities covering the range of 10 sup 8 -10 sup 1 sup 4 cm sup - sup 3. Relative spectral line intensities are calculated. Proton excitation rates between the lowest three levels have been included in the statistical equilibrium equations. The predicted Ne III line intensi...

  12. Acetylation within the N- and C-Terminal Domains of Src Regulates Distinct Roles of STAT3-Mediated Tumorigenesis. (United States)

    Huang, Chao; Zhang, Zhe; Chen, Lihan; Lee, Hank W; Ayrapetov, Marina K; Zhao, Ting C; Hao, Yimei; Gao, Jinsong; Yang, Chunzhang; Mehta, Gautam U; Zhuang, Zhengping; Zhang, Xiaoren; Hu, Guohong; Chin, Y Eugene


    Posttranslational modifications of mammalian c-Src N-terminal and C-terminal domains regulate distinct functions. Myristoylation of G 2 controls its cell membrane association and phosphorylation of Y419/Y527 controls its activation or inactivation, respectively. We provide evidence that Src-cell membrane association-dissociation and catalytic activation-inactivation are both regulated by acetylation. In EGF-treated cells, CREB binding protein (CBP) acetylates an N-terminal lysine cluster (K5, K7, and K9) of c-Src to promote dissociation from the cell membrane. CBP also acetylates the C-terminal K401, K423, and K427 of c-Src to activate intrinsic kinase activity for STAT3 recruitment and activation. N-terminal domain phosphorylation (Y14, Y45, and Y68) of STAT3 by c-Src activates transcriptionally active dimers of STAT3. Moreover, acetyl-Src translocates into nuclei, where it forms the Src-STAT3 enhanceosome for gene regulation and cancer cell proliferation. Thus, c-Src acetylation in the N-terminal and C-terminal domains play distinct roles in Src activity and regulation. Significance: CBP-mediated acetylation of lysine clusters in both the N-terminal and C-terminal regions of c-Src provides additional levels of control over STAT3 transcriptional activity. Cancer Res; 78(11); 2825-38. ©2018 AACR . ©2018 American Association for Cancer Research.

  13. Soil properties influencing phytoparasitic nematode population on Chilean vineyards Propiedades del suelo que influyen en la población de nematodos fitoparásitos en viñedos de Chile

    Directory of Open Access Journals (Sweden)

    Mario Fajardo P


    Full Text Available Lifecycle of phytoparasitic nematode takes place in the rhizosphere, therefore their breeding, parasitism and mobility dynamics are inevitably influenced by the soil-root interaction, A study was performed to evaluate the influence of Vitis rootstocks to some plant parasitic nematodes under different soil conditions. Nematode populations were assessed in Vitis vinifera L. var ‘Chardonnay’ plants grafted on two rootstocks (K5BB, SO4 and ungrafted ‘Chardonnay’ as a control in three diferent alluvial soils in the central zone of Chile. Soils were two Inceptisols of the Casablanca Valley (Valparaíso Region, the first one without soil structure and with a densification zone in depth (S1 and the second one with sandy textural class (S3. A third soil was a Mollisol (S2 more structured than the others, situated on a locality of Melipilla (Metropolitan Region. The soils were characterized physically and morphologically and nematode genera were identified and counted using a dissecting microscope. ‘Chardonnay’ presented the highest population of Meloidogyne spp. on the three soil conditions but only significant in S2 soil. The population of Xiphinema spp. and Mesocriconema xenoplax were not representative enough to relate them with either soil or the different rootstocks. The amount of Meloidogyne spp. was inversely related with the sand content but positively related with the more structured soil. The stepwise regressions resulted useful when relating nematode populations with multiple soil factors.El ciclo de vida de los nematodos fitoparásitos ocurre en la rizósfera, por lo tanto, sus dinámicas de alimentación, parasitismo y movilidad están inevitablemente influenciadas por la interacción suelo-raíz. Se llevó a cabo un estudio para evaluar la respuesta de diferentes portainjertos de Vitis frente a algunas poblaciones de nematodos fitoparásitos en diferentes tipos de suelos. Se determinaron las poblaciones de nematodos fitopar

  14. PKL/K9, Refill and Reflood Experiment in a Simulated PWR Primary System (PKL)

    International Nuclear Information System (INIS)


    reflood process within a pressure vessel considering the influence of the simulated primary loops of a pressurized water reactor (PWR). As to the number of rods and the cross-sections of vessel and components the PKL test-facility is scaled down to 1:134 of a typical West Germany PWR. The elevations and locations have been designed in order to reproduce substantially the original extension. Compared to other cold leg injection tests in the same facility (K5. 3a, K5. 4a) test K9 differs in a uniform radial power profile in the core, an increased temperature of injected Emergency Core Cooling (ECC) water (feed water, from 35 to 53 deg. C), an injection mass flow rate highly reduced to 1:3, and a history of it corresponding to that of a typical US-PWR, on the average somewhat higher maximum initial temperatures in the bundle, and a smaller bundle heating power during the initial phase

  15. Efeito da omissão de macronutrientes e boro no crescimento, nos sintomas de deficiências nutricionais e na composição mineral de plantas de camucamuzeiro Effect of omission of macronutrient and boron on growth, on symptoms of nutritional deficiency and mineral composition in camucamuzeiro plants (Myrciaria dubia

    Directory of Open Access Journals (Sweden)

    Ismael de Jesus Matos Viégas


    Full Text Available Com o objetivo de avaliar o efeito da omissão de macronutrientes e do micronutriente boro no crescimento, nos sintomas de deficiências nutricionais e na composição mineral em plantas de camucamuzeiro, conduziu-se experimento em casa de vegetação, mediante a técnica do elemento faltante. O delineamento experimental foi o inteiramente casualizado, com quatro repetições e oito tratamentos, sendo completo (N, P, K, Ca, Mg, S e micronutrientes e omissão individual de N, P, K, Ca, Mg, S e B. Os sintomas visuais de deficiências foram, de modo geral, de fácil caracterização para todos os nutrientes. Com exceção do fósforo, as omissões dos demais nutrientes afetaram a produção de matéria seca, quando comparados ao tratamento completo. Com base nos teores em g kg-1, dos macronutrientes, e em mg kg-1, do micronutriente boro nas folhas, infere-se em uma primeira aproximação dos valores adequados (completo, ou seja: 16,9 a 18,2 de N ; 1,2 a 1,9 de P; 5,2 a 6,0 de K; 9,9 a 11,7 de Ca; 1,4 a 3,6 de Mg; 2,4 a 2,8 de S ; 8,4 a 9,5 de B e do deficiente (omissão , 6,5 a 7,9 de N ; =0,9 de P; =1,7 de K ; 5,4 a 6,5 de Ca; =0,7 de Mg; 0,7 a 1,2 de S e 1,1 a 1,9 de BThe effects of omission of macronutrient and boron on growth, on symptoms of nutritional deficiency and mineral composition of plants of "camucamuzeiro" were evaluated. The experiment was carried out in a greenhouse, by means of the missing element technique. The experimental design was completely randomized, with eight treatments and four repetitions, including complete (N, P, K, Ca, Mg, S and micronutrients and individual omission of N, P, K, Ca, Mg, S and B. The visual symptoms of deficiency were easily characterized for all the nutrients. Excepting for P, dry mass was affected by the omission of all the other nutrients, when compared with the complete treatment. Based on the macronutrient (g kg-1 and on the micronutrient boron (mg kg-1 on leaves contents, a first approach of the

  16. Effect of cyanato, azido, carboxylato, and carbonato ligands on the formation of cobalt(II) polyoxometalates: characterization, magnetic, and electrochemical studies of multinuclear cobalt clusters. (United States)

    Lisnard, Laurent; Mialane, Pierre; Dolbecq, Anne; Marrot, Jérôme; Clemente-Juan, Juan Modesto; Coronado, Eugenio; Keita, Bineta; de Oliveira, Pedro; Nadjo, Louis; Sécheresse, Francis


    Five Co(II) silicotungstate complexes are reported. The centrosymmetric heptanuclear compound K(20)[{(B-beta-SiW(9)O(33)(OH))(beta-SiW(8)O(29)(OH)(2))Co(3)(H(2)O)}(2)Co(H(2)O)(2)]47 H(2)O (1) consists of two {(B-beta-SiW(9)O(33)(OH))(beta-SiW(8)O(29)(OH)(2))Co(3)(H(2)O)} units connected by a {CoO(4)(H(2)O)(2)} group. In the chiral species K(7)[Co(1.5)(H(2)O)(7))][(gamma-SiW(10)O(36))(beta-SiW(8)O(30)(OH))Co(4)(OH)(H(2)O)(7)]36 H(2)O (2), a {gamma-SiW(10)O(36)} and a {beta-SiW(8)O(30)(OH)} unit enclose a mononuclear {CoO(4)(H(2)O)(2)} group and a {Co(3)O(7)(OH)(H(2)O)(5)} fragment. The two trinuclear Co(II) clusters present in 1 enclose a mu(4)-O atom, while in 2 a mu(3)-OH bridging group connects the three paramagnetic centers of the trinuclear unit, inducing significantly larger Co-L-Co (L=mu(4)-O (1), mu(3)-OH (2)) bridging angles in 2 (theta(av(Co-L-Co))=99.1 degrees ) than in 1 (theta(av(Co-L-Co))=92.8 degrees ). Weaker ferromagnetic interactions were found in 2 than in 1, in agreement with larger Co-L-Co angles in 2. The electrochemistry of 1 was studied in detail. The two chemically reversible redox couples observed in the positive potential domain were attributed to the redox processes of Co(II) centers, and indicated that two types of Co(II) centers in the structure were oxidized in separate waves. Redox activity of the seventh Co(II) center was not detected. Preliminary experiments indicated that 1 catalyzes the reduction of nitrite and NO. Remarkably, a reversible interaction exists with NO or related species. The hybrid tetranuclear complexes K(5)Na(3)[(A-alpha-SiW(9)O(34))Co(4)(OH)(3)(CH(3)COO)(3)]18 H(2)O (3) and K(5)Na(3)[(A-alpha-SiW(9)O(34))Co(4)(OH)(N(3))(2)(CH(3)COO)(3)]18 H(2)O (4) were characterized: in both, a tetrahedral {Co(4)(L(1))(L(2))(2)(CH(3)COO)(3)} (3: L(1)=L(2)=OH; 4: L(1)=OH, L(2)=N(3)) unit capped the [A-alpha-SiW(9)O(34)](10-) trivacant polyanion. The octanuclear complex K(8)Na(8)[(A-alpha-SiW(9)O(34))(2)Co(8)(OH)(6)(H(2)O)(2)(CO(3


    Directory of Open Access Journals (Sweden)

    Humberto Actis Zaidan


    Full Text Available Nas abordagens biotecnológicas de propagação de plantas, os meios de cultura devem ter uma composição química adequada à essa finalidade permitindo a otimização da produção. Como a bananeira (Musa sp. é exigente em potássio, a busca do nível adequado desse macronutriente envolve não somente o comprometimento com o nível dos outros nutrientes (balanço iônico, mas também a relação entre eles. Para acompanhar os efeitos fisiológicos dos vários teores de K, explantes caulinares dos cvs. "Nanica" e "Prata Anã" foram cultivados em meio MS modificado, em presença de BAP (2,5 mg/L, sacarose, vitaminas e agar, em seis diferentes concentrações de K: 5, 10, 15, 20, 25 e 30 mM, sendo 20 mM a concentração de K no MS básico. Foram feitas análises de massa de matéria seca (MMS, K na parte aérea, raiz e plântulas inteiras, e determinado o número de plântulas, aos 90 dias de idade. Após início de cultivo todos os parâmetros foram analisados segundo um delineamento inteiramente casualizado. As plântulas que se desenvolveram em baixas concentrações de K apresentaram sintomas visuais de deficiência de K, como clorose e necrose nas folhas mais velhas. Os cultivares apresentaram diferenças quantitativas entre si, relacionados à concentração de K presente no meio de cultura, seja em relação aos valores de MMS, ou quanto ao número de plântulas formadas. Em ambos os cultivares, foi observada uma relação direta entre o desenvolvimento de plântulas e as concentrações de K com otimização ao redor de 15 a 20 mM de K.The chemical composition of culture growth media to micropropagate plants should be devised to allow the best quality and high production of plant material. One of the challenges is to optimize the ionic balance involving mineral nutrients. As banana (Musa sp. requires high levels of potassium, the optimum level was investigated. Shoot apexes of two banana cultivars 'Nanica' and 'Prata Anã' were

  18. Sintomas de deficiência nutricional em mudas de Acacia holosericea em resposta à omissão de macronutrientes Symptoms of nutritional deficiency in seedlings of Acacia holosericea submitted to absence of macronutrients

    Directory of Open Access Journals (Sweden)

    Tathiane Santi Sarcinelli


    Full Text Available A Acacia holosericea é uma espécie leguminosa arbórea bastante utilizada na recuperação de áreas degradadas. O conhecimento dos sintomas de deficiência nutricional apresentados por esta espécie possibilita a identificação e a correção de deficiências em exemplares plantados em substratos degradados. Os objetivos deste trabalho foram caracterizar a sintomatologia visual de carências de macronutrientes e avaliar a produção de biomassa e o acúmulo de nutrientes nas raízes e na parte aérea de mudas de Acacia holosericea, submetidas a diferentes soluções nutritivas com exclusão de macronutrientes. Os tratamentos constituíram-se de sete soluções nutritivas: 1 solução completa (SC; 2 SC -N; 3 SC -P; 4 SC -K; 5 SC -Ca; 6 SC -Mg; e 7 SC -S. Os tratamentos -N e Mg foram os que mais afetaram a produção total de biomassa. O decréscimo de produção manifestou-se na seguinte ordem: -N = -Mg > -K > -S > -Ca > SC > -P. As plantas do tratamento -N formaram nódulos no sistema radicular, exibindo teores foliares de N maiores que as plantas do tratamento SC. Com exceção dos tratamentos SC e -P, todos os outros apresentaram sintomas de deficiência. A ausência de S alterou a disposição natural dos filódios novos da A. holosericea.Acacia holosericea is a leguminous species that has been extensively used in land reclamation programs. The knowledge of its nutritional symptoms allows the identification and correction of deficiencies in individuals planted in degraded substrates. The aim of this work was to characterize the behavior of seedlings of A. holosericea submitted to different nutritional solutions with absence of macronutrients. The treatments consisted of seven nutritional solutions: 1 Complete solution (SC; 2 SC - N; 3 SC -P; 4 SC -K; 5 SC -Ca; 6 SC -Mg; 7 SC -S. Treatments -N and -Mg were the ones that most affected biomass production. Decrease in biomass production occurred in the following order: -N = -Mg > -K > -S

  19. Radiolysis of aqueous solutions of nucleosides halogenated at the sugar moiety

    Energy Technology Data Exchange (ETDEWEB)

    Hissung, A; Isildar, M; von Sonntag, C [Max-Planck-Institut fuer Kohlenforschung, Muelheim an der Ruhr (Germany, F.R.). Inst. fuer Strahlenforschung; Witzel, H [Biochemisches Institut der Westfaelischen Wilhelms-Universitaet, Muenster, West Germany


    The pulse radiolysis of aqueous solutions of nucleosides halogenated at the sugar moiety (2'-bromo-2'-deoxyuridine 4, 3'-deoxy-3'-iodothymidine 5, 5'-deoxy-5'-iodouridine 6) has been studied. G(Hal) were determined by conductometry varying the experimental conditions (pH, saturation with Ar, N/sub 2/O or air, addition of t-butanol). The results indicate that solvated electrons both add to the nucleobases and eliminate halogen ions from the halogenated sugar moiety. In the case of 4(and possibly of 5) the radical anion of the base transfers (k approximately 10/sup 5/s/sup -1/) an electron to the sugar-bound halogen atom thus cleaving the C-Hal bond. In competition with this reaction there is a protonation of the radical anion of the base by protons and by water. For the latter reaction constant of k = 5 x 10/sup 3/ M/sup -1/s/sup -1/ was estimated. Compound 4 has also been investigated by product analysis after 60-Co-..gamma..-irradiation. In aerated solutions erythrose is formed with a G-value of 0.12. Its precursor radical is the 2'-radical generated from 4 by dissociative electron capture which reacts with O/sub 2/ to the corresponding peroxyl radical. Erythrose is formed after a sequence of reactions, one of which involves the scission of the C-1'-C-2'bond. Under this condition G(HBr) as measured by pulse radiolysis is 0.8. Thus erythrose is formed in 15 per cent yield with respect to its precursor radical. This result is of importance in assessing the precursor radical of a similar product observed in irradiated DNA.

  20. Economic Feasibility of Staffing the Intensive Care Unit with a Communication Facilitator. (United States)

    Khandelwal, Nita; Benkeser, David; Coe, Norma B; Engelberg, Ruth A; Curtis, J Randall


    In the intensive care unit (ICU), complex decision making by clinicians and families requires good communication to ensure that care is consistent with the patients' values and goals. To assess the economic feasibility of staffing ICUs with a communication facilitator. Data were from a randomized trial of an "ICU communication facilitator" linked to hospital financial records; eligible patients (n = 135) were admitted to the ICU at a single hospital with predicted mortality ≥30% and a surrogate decision maker. Adjusted regression analyses assessed differences in ICU total and direct variable costs between intervention and control patients. A bootstrap-based simulation assessed the cost efficiency of a facilitator while varying the full-time equivalent of the facilitator and the ICU mortality risk. Total ICU costs (mean 22.8k; 95% CI, -42.0k to -3.6k; P = 0.02) and average daily ICU costs (mean, -0.38k; 95% CI, -0.65k to -0.11k; P = 0.006)] were reduced significantly with the intervention. Despite more contacts, families of survivors spent less time per encounter with facilitators than did families of decedents (mean, 25 [SD, 11] min vs. 36 [SD, 14] min). Simulation demonstrated maximal weekly savings with a 1.0 full-time equivalent facilitator and a predicted ICU mortality of 15% (total weekly ICU cost savings, $58.4k [95% CI, $57.7k-59.2k]; weekly direct variable savings, $5.7k [95% CI, $5.5k-5.8k]) after incorporating facilitator costs. Adding a full-time trained communication facilitator in the ICU may improve the quality of care while simultaneously reducing short-term (direct variable) and long-term (total) health care costs. This intervention is likely to be more cost effective in a lower-mortality population.