WorldWideScience

Sample records for k-9 sts content

  1. The impact of a dedicated Science-Technology-Society (STS) course on student knowledge of STS content

    Science.gov (United States)

    Barron, Paul E.

    In the last half century, public awareness of issues such as population growth, environmental pollution and the threat of nuclear war has pressured science education to reform to increase student social responsibility. The emerging Science-Technology-Society (STS) movement addressed these concerns by developing numerous strategies and curricula. Considerable diagnostic research has been conducted on student knowledge of the nature of science, but not on the wider scope of STS content (e.g., the nature of science and technology and their interactions with society). However, researchers have not widely studied the impact of comprehensive STS curricula on students' knowledge of STS content nor the nature of STS teaching practice that influenced this knowledge gain. This study examined student success and teacher performance in a special STS course in Ontario, Canada. Research questions focused on the STS content knowledge gain by students completing this course and the impact of the STS teachers' teaching practices on this knowledge gain. Student data were collected using pre-course and post-course assessments of students' STS content knowledge. Teacher data were obtained using semi-structured interviews, classroom observations and videotapes. Statistical analysis indicated that, after completing the STS course, students significantly increased their STS content knowledge as measured by the Views on Science Technology Society instrument. Gender and academic achievement had no significant impact on this knowledge gain, implying that this course, as taught by these teachers, could appeal to a wide range of students as a general education course. The second part of the study indicated that detailed research is needed on the relationship between STS teaching practice and student STS content knowledge gain. The small sample size prevents generalizations but initial indications show that factors such constructivist teaching practices and strong teacher STS content knowledge

  2. K9传奇

    Institute of Scientific and Technical Information of China (English)

    周文峰

    2004-01-01

    @@ 源远流长的家世 K9这个说法我们中国人不大熟悉,对其意思也不甚明了,但是,在美国、俄罗斯等国,K9却是一个非常大众化的用词.实际上,"K9"就是指警犬和军犬.

  3. K9的由来

    Institute of Scientific and Technical Information of China (English)

    张四峰

    2015-01-01

    <正>K9在美国、俄罗斯等国,是一个非常大众化的用词。实际上,"K9"就是指警犬和军犬。这个称呼还有一段来历。第二次世界大战时,美国看到第一次世界大战期间军犬在战场上发挥的巨大作用,于是也开始组建自己的拥有2万只军犬的特种部队,用于执行侦察、放哨、探测地雷、警卫等军事任务。但是,如何给这支部队编号呢?于是就有人想到犬的英文单词Canine的谐音K9。这个编号深受官方和公众的欢迎,很快就风靡开来。在美

  4. K9巡逻队

    Institute of Scientific and Technical Information of China (English)

    朱英

    2015-01-01

    某警察局以其精锐的警犬部队(即K9巡逻队)而闻名,但在最近的一次行动中却遭遇了尴尬。一个金发女子下班回到家中,发现自己的家被洗劫一空,感到非常震惊。她立即打电话向警察局报案。

  5. Characterization of Volume F Trash from Four Recent STS Missions: Weights, Categorization, Water Content

    Science.gov (United States)

    Strayer, Richard F.; Hummerick, Mary E.; Richards, Jeffrey T.; McCoy, LaShelle E.; Roberts, Michael S.; Wheeler, Raymond M.

    2011-01-01

    The fate of space-generated solid wastes, including trash, for future missions is under consideration by NASA. Several potential treatment options are under consideration and active technology development. Potential fates for space-generated solid wastes are: Storage without treatment; storage after treatment(s) including volume reduction, water recovery, sterilization, and recovery plus recycling of waste materials. Recycling might be important for partial or full closure scenarios because of the prohibitive costs associated with resupply of consumable materials. For this study, we determined the composition of trash returned from four recent STS missions. The trash material was 'Volume F' trash and other trash, in large zip-lock bags, that accompanied the Volume F trash. This is the first of two submitted papers on these wastes. This one will cover trash content, weight and water content. The other will report on the microbial Characterization of this trash. STS trash was usually made available within 2 days of landing at KSC. The Volume F bag was weighed, opened and the contents were catalogued and placed into one of the following categories: food waste (and containers), drink containers, personal hygiene items - including EVA maximum absorbent garments (MAGs)and Elbow packs (daily toilet wipes, etc), paper, and packaging materials - plastic firm and duct tape. Trash generation rates for the four STS missions: Total wet trash was 0.602 plus or minus 0.089 kg(sub wet) crew(sup -1) d(sup -1) containing about 25% water at 0.154 plus or minus 0.030 kg(sub water) crew(sup -1) d(sup -1) (avg plus or minus stdev). Cataloguing by category: personal hygiene wastes accounted for 50% of the total trash and 69% of the total water for the four missions; drink items were 16% of total weight and 16% water; food wastes were 22% of total weight and 15% of the water; office waste and plastic film were 2% and 11% of the total waste and did not contain any water. The results can be

  6. Use of K9 units in healthcare: a history.

    Science.gov (United States)

    Sarnese, Paul M

    2016-01-01

    The use of K9 units within healthcare facilities will continue to increase as the violence within healthcare increases. K9s are a wise investment and are cost-effective. K9 units deter and prevent crime and violence at facilities. The typical K9 will be utilized for eight to ten years. The research demonstrates that facilities that have deployed K9 units have seen a reduction in crime and violence. A well trained K9 can not only patrol the exterior of a healthcare facility but also the patient care and service areas. As more hospitals and healthcare facilities look at non-lethal weapons to protect their officers, staff and guests, the use of K9 within healthcare will continue to rise.

  7. K9初级课堂 关于K9 sports的基础问题

    Institute of Scientific and Technical Information of China (English)

    sunbird

    2006-01-01

    K9训练营已经开办近一年,小编是越来越了解这个K9 sports,进而越来越喜欢,于是想做得更专业,却不小心进入一个误区,很多新读者看着小编每次报道的K9比赛难免一头雾水,心中有个大大的问号,到底什么是K9 sports呀?本期K9训练营就来解答新读者们的疑惑,也为以后咱K9 sports更好地发展开个好头!

  8. Refractive index of K9 Glass under Shock Loading

    Science.gov (United States)

    Hu, Changming; Wang, Xiang; Cai, Lingcang; Liu, Cangli

    2013-06-01

    We study K9 glass refraction index under shock loading conducted on powder gun,all experimental tests are plate impact loading. The impact veceloty range from 300m/s to 1200m/s, and the measure method is laser interferometer Photon Doppler Velocimetry(PDV) to measure the particle velocity both at the impact interface and free surface, The shock pressure from 2 GPa to 8 GPa, values for refraction are found from velocity corrections that must be made to account for refraction-index changes in the K9 glass due to shock wave motion. Experiment results show that refraction-index of K9 glass changes with the shock pressure in line relations, it can be as measure window to study the interesting materials under 10 GPa during the shock loading.

  9. 起亚全新车型K9

    Institute of Scientific and Technical Information of China (English)

    肖昕

    2012-01-01

    车型平台源自雅科仕。外观造型出自大师手笔一如K2与瑞纳、K5与索纳塔的兄弟关系,K9也自然而然地继承了雅科仕的车型平台。不过出自设计大师彼得·希瑞尔手笔的K9显然不像保守的雅科仕那么死气沉沉,

  10. K9与空警协同作战

    Institute of Scientific and Technical Information of China (English)

    李钢; 游海霞

    2011-01-01

    在美国、俄罗斯等国,用"K9"指代警犬和军犬.这个称呼始于第二次世界大战.当时,美国看到第一次世界大战期间军犬在战场上发挥的巨大作用,于是也开始组建自己的拥有2万只军犬的特种部队,用于执行侦察、放哨、探测地雷、警卫等军事任务.但是,如何给这支部队编号呢?于是就有人想到犬的英文单词Canine的谐音K9.这个编号深受官方和公众的欢迎,很快就风靡开来.

  11. The K9 On-Board Rover Architecture

    Science.gov (United States)

    Bresina, John L.; Bualat, Maria; Fair, Michael; Washington, Richard; Wright, Anne

    2006-01-01

    This paper describes the software architecture of NASA Ames Research Center s K9 rover. The goal of the onboard software architecture team was to develop a modular, flexible framework that would allow both high- and low-level control of the K9 hardware. Examples of low-level control are the simple drive or pan/tilt commands which are handled by the resource managers, and examples of high-level control are the command sequences which are handled by the conditional executive. In between these two control levels are complex behavioral commands which are handled by the pilot, such as drive to goal with obstacle avoidance or visually servo to a target. This paper presents the design of the architecture as of Fall 2000. We describe the state of the architecture implementation as well as its current evolution. An early version of the architecture was used for K9 operations during a dual-rover field experiment conducted by NASA Ames Research Center (ARC) and the Jet Propulsion Laboratory (JPL) from May 14 to May 16, 2000.

  12. H3 trimethyl K9 and H3 acetyl K9 chromatin modifications are associated with class switch recombination.

    Science.gov (United States)

    Kuang, Fei Li; Luo, Zhonghui; Scharff, Matthew D

    2009-03-31

    Class switch recombination (CSR) involves a DNA rearrangement in the Ig heavy chain (IgH) gene that allows the same variable (V) region to be expressed with any one of the downstream constant region (C) genes to encode antibodies with many different effector functions. One hypothesis for how CSR is targeted to different C region genes is that histone modifications increase accessibility and/or recruit activation-induced cytosine deaminase (AID) and its associated processes to particular donor and recipient switch regions. In this work, we identified H3 acetyl K9 and H3 trimethyl K9 as histone modifications that correlate with the recombining pair of donor and recipient switch regions. The appearance of H3 trimethyl K9 is surprising because usually it is thought to mark silent genes and heterochromatin. Nevertheless, the time course of appearance of these histone modifications, the regions in IgH they associate with, and their appearance independent of AID damage suggest that both modifications play a role in targeting CSR.

  13. Main: 1K9B [RPSD[Archive

    Lifescience Database Archive (English)

    Full Text Available 1K9B 大豆 Soybean Glycine max (L.) Merrill Bowman-Birk Type Proteinase Inhibitor Precursor Glycine Ma...x Molecule: Bowman-Birk Type Proteinase Inhibitor; Chain: A; Synonym: Bbi; Other_details: Fr....O.Essen, G.Wenzl, Y.M.Kim, P.Flecker Crystal Structure Of The Bifunctional Soybean Bowma...n-Birk Inhibitor At 0.28 Nm Resolution. Structural Peculiarities In A Folded Protein Conformation Eu...877; Prot_inh_BBI.|Pfam; PF00228; Bowman-Birk_leg; 2.|SMART; SM00269; BowB; 1.|PROSITE; PS00281; BOWMAN_BIRK

  14. Structural biology of human H3K9 methyltransferases.

    Directory of Open Access Journals (Sweden)

    Hong Wu

    Full Text Available UNLABELLED: SET domain methyltransferases deposit methyl marks on specific histone tail lysine residues and play a major role in epigenetic regulation of gene transcription. We solved the structures of the catalytic domains of GLP, G9a, Suv39H2 and PRDM2, four of the eight known human H3K9 methyltransferases in their apo conformation or in complex with the methyl donating cofactor, and peptide substrates. We analyzed the structural determinants for methylation state specificity, and designed a G9a mutant able to tri-methylate H3K9. We show that the I-SET domain acts as a rigid docking platform, while induced-fit of the Post-SET domain is necessary to achieve a catalytically competent conformation. We also propose a model where long-range electrostatics bring enzyme and histone substrate together, while the presence of an arginine upstream of the target lysine is critical for binding and specificity. ENHANCED VERSION: This article can also be viewed as an enhanced version in which the text of the article is integrated with interactive 3D representations and animated transitions. Please note that a web plugin is required to access this enhanced functionality. Instructions for the installation and use of the web plugin are available in Text S1.

  15. Data of evolutionary structure change: 1K9PA-1CNPA [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1K9PA-1CNPA 1K9P 1CNP A A -ACPLDQAIGLLVAIFHKYSGREGDKHTLSKKELKELIQ...D>1K9P A 1K9PA IGSKL-QDAEI 1CNP A 1CNPA VAL CA 413 -13.432999610900879 15.65699958801269...5 50.41999816894531 rallel> 0.11400000005960464 0.9580000042915344 <

  16. 俄罗斯进口 K9焦煤在本钢的应用研究%Application research of Russian imported K9 coking coal in Bensteel

    Institute of Scientific and Technical Information of China (English)

    邢毅; 王文光; 翟向军; 韩颖; 李利凯

    2015-01-01

    通过质量检测、煤的岩相分析、40 kg试验焦炉炼焦试验,对俄罗斯进口K9焦煤的煤质特性和结焦特性进行了研究。结果表明K9煤低灰、低硫,为单一煤层煤,煤质稳定,配煤炼焦时有利于焦炭质量稳定,提高焦炭平均粒度和抗碎强度。在使用K9焦煤时,还可以配入高硫肥煤。%Russian imported K9 coking coal was investigated on coal quality and coking property by means of quality detection, petrographic analysis of coal and 40kg testing coke oven test.The result in-dicated that K9 coal was featured with low ash, low sulfur and stable quality, belonging to single coal seam.So it is beneficial to stabilize coke quality and improve coke average particle size and crushing strength in coal blending and coking.Meanwhile, fat coal with high sulfur content can be used in co-king.

  17. Architecture for Control of the K9 Rover

    Science.gov (United States)

    Bresina, John L.; Bualat, maria; Fair, Michael; Wright, Anne; Washington, Richard

    2006-01-01

    Software featuring a multilevel architecture is used to control the hardware on the K9 Rover, which is a mobile robot used in research on robots for scientific exploration and autonomous operation in general. The software consists of five types of modules: Device Drivers - These modules, at the lowest level of the architecture, directly control motors, cameras, data buses, and other hardware devices. Resource Managers - Each of these modules controls several device drivers. Resource managers can be commanded by either a remote operator or the pilot or conditional-executive modules described below. Behaviors and Data Processors - These modules perform computations for such functions as planning paths, avoiding obstacles, visual tracking, and stereoscopy. These modules can be commanded only by the pilot. Pilot - The pilot receives a possibly complex command from the remote operator or the conditional executive, then decomposes the command into (1) more-specific commands to the resource managers and (2) requests for information from the behaviors and data processors. Conditional Executive - This highest-level module interprets a command plan sent by the remote operator, determines whether resources required for execution of the plan are available, monitors execution, and, if necessary, selects an alternate branch of the plan.

  18. Recognition of H3K9 methylation by GLP is required for efficient establishment of H3K9 methylation, rapid target gene repression, and mouse viability

    Science.gov (United States)

    Liu, Nan; Zhang, Zhuqiang; Jiang, Yonghua; Meng, Lingjun; Xiong, Jun; Zhao, Zuodong; Zhou, Xiaohua; Li, Jia; Li, Hong; Zheng, Yong; Chen, She; Cai, Tao; Gao, Shaorong

    2015-01-01

    GLP and G9a are major H3K9 dimethylases and are essential for mouse early embryonic development. GLP and G9a both harbor ankyrin repeat domains that are capable of binding H3K9 methylation. However, the functional significance of their recognition of H3K9 methylation is unknown. Here, we report that the histone methyltransferase activities of GLP and G9a are stimulated by neighboring nucleosomes that are premethylated at H3K9. These stimulation events function in cis and are dependent on the H3K9 methylation binding activities of ankyrin repeat domains of GLP and G9a. Disruption of the H3K9 methylation-binding activity of GLP in mice causes growth retardation of embryos, ossification defects of calvaria, and postnatal lethality due to starvation of the pups. In mouse embryonic stem cells (ESCs) harboring a mutant GLP that lacks H3K9me1-binding activity, critical pluripotent genes, including Oct4 and Nanog, display inefficient establishment of H3K9me2 and delayed gene silencing during differentiation. Collectively, our study reveals a new activation mechanism for GLP and G9a that plays an important role in ESC differentiation and mouse viability. PMID:25637356

  19. The SUVR4 histone lysine methyltransferase binds ubiquitin and converts H3K9me1 to H3K9me3 on transposon chromatin in Arabidopsis.

    Directory of Open Access Journals (Sweden)

    Silje V Veiseth

    2011-03-01

    Full Text Available Chromatin structure and gene expression are regulated by posttranslational modifications (PTMs on the N-terminal tails of histones. Mono-, di-, or trimethylation of lysine residues by histone lysine methyltransferases (HKMTases can have activating or repressive functions depending on the position and context of the modified lysine. In Arabidopsis, trimethylation of lysine 9 on histone H3 (H3K9me3 is mainly associated with euchromatin and transcribed genes, although low levels of this mark are also detected at transposons and repeat sequences. Besides the evolutionarily conserved SET domain which is responsible for enzyme activity, most HKMTases also contain additional domains which enable them to respond to other PTMs or cellular signals. Here we show that the N-terminal WIYLD domain of the Arabidopsis SUVR4 HKMTase binds ubiquitin and that the SUVR4 product specificity shifts from di- to trimethylation in the presence of free ubiquitin, enabling conversion of H3K9me1 to H3K9me3 in vitro. Chromatin immunoprecipitation and immunocytological analysis showed that SUVR4 in vivo specifically converts H3K9me1 to H3K9me3 at transposons and pseudogenes and has a locus-specific repressive effect on the expression of such elements. Bisulfite sequencing indicates that this repression involves both DNA methylation-dependent and -independent mechanisms. Transcribed genes with high endogenous levels of H3K4me3, H3K9me3, and H2Bub1, but low H3K9me1, are generally unaffected by SUVR4 activity. Our results imply that SUVR4 is involved in the epigenetic defense mechanism by trimethylating H3K9 to suppress potentially harmful transposon activity.

  20. Dangerous R loops form in the absence of H3K9 methylation

    DEFF Research Database (Denmark)

    Salcini, Anna Elisabetta

    2016-01-01

    Methylation of histone H3 on lysine 9 (H3K9) is a hallmark of transcriptionally inactive heterochromatin that is deregulated in pathological conditions. A new study shows that complete loss of H3K9 methylation in Caenorhabditis elegans leads to derepression of repetitive elements and formation...

  1. Application of Geologic Mapping Based on MapGIS K9%基于MapGIS K9的地质制图的应用

    Institute of Scientific and Technical Information of China (English)

    陈立

    2011-01-01

    Geologic mapping is an important component in geographic information system. The MapGIS K9 in the geologic mapping was described briefly and the process of geologic mapping was also expounded in this article.%地质制图是地理信息系统中的一项重要的组成部分,文章简要介绍了地理信息地质制图中的MapGIS K9,并对地质制图过程进行叙述.

  2. The application of MAPGIS K9 in geological mapping%MAPGIS K9在地质制图中的应用

    Institute of Scientific and Technical Information of China (English)

    冯娟; 翟伟芳; 赵伟娜

    2016-01-01

    The product name of MAPGIS is released digital group, is China's geographic information system with full independent intellectual copyright software platform. MAPGIS K9 help drawings, graphics editing and graphics processing and other functions, is the first selection of digital industry of our country geological mapping tool. Combined with practical work, this paper introduces the MAPGIS K9 at Grid correction, point parameters editor, line editor, the pologon parameters editor, map layout design of digital mapping method and the skill.%MAPGIS是中地数码集团的产品名称,是我国具有完全自主知识版权的地理信息系统软件平台。MAPGIS K9具有机助制图、图形编辑和图形处理等多种功能,是我国地质行业数字化制图的首选工具。本文结合工作实例,介绍了MAPGIS K9在栅格数据校正、点参数编辑、线参数编辑、面参数编辑、地图版面设计等方面的数字制图方法和技巧。

  3. Effect of amorphous C films deposited by RF magnetron sputtering on smoothing K9 glass substrate

    Science.gov (United States)

    Deng, Songwen; Qi, Hongji; Wei, Chaoyang; Yi, Kui; Fan, Zhengxiu; Shao, Jianda

    2009-12-01

    Soft X-ray multilayer reflectors must be deposited on super-smooth surface such as super-polished silicon wafers or glasses, which are complicate, time-consuming and expensive to produce. To overcome this shortage, C films deposited by RF magnetron sputtering were considered to smooth the K9 glass substrates' surface in the present paper. The structure of C films was systematically studied by XRD and Raman spectrum. The surface morphology and rms-roughness were obtained by AFM. Then, we calculated the impact of the C layers on the reflectivity curve of Mo/Si soft X-ray multilayer reflector around 13.5 nm. The C films exhibit typical amorphous state. With the increasing of power and thickness, the content of sp3 hybrid bonding decreases while the amount or size of well-organized graphite clusters increases. The surface rms-roughness decreases from 2.4 nm to 0.62 nm after smoothed by an 80 nm thick C layer deposited in 500 W, which is the smoothest C layer surface we have obtained. The calculation results show that the theoretical normal incidence reflectivity of Mo/Si multilayer at 13.5 nm increases from 7% to 63%.

  4. Study on damage of K9 glass under 248nm ultraviolet pulsed laser irradiation

    Science.gov (United States)

    Wang, Xi; Fang, Xiaodong

    2015-04-01

    The damage of K9 glass under 248nm ultraviolet pulsed laser irradiation was studied. The laser pulse energy was kept within the range of 60mJ to 160mJ, and the repetition rate was adjusted within the range of 1Hz to 40Hz. The damage morphologies of single-pulse and multi-pulse laser irradiation were characterized by optical microscope, and the damage mechanism was discussed. The experimental results indicated that the damage of K9 glass irradiated by 248nm ultraviolet laser mainly followed the thermal-mechanical coupling mechanism and the damage threshold of K9 glass was 2.8J/cm2. The intensity of damage area increased gradually with the increase of the laser pulse number. It was shown that accumulation effect of laser induced damage to K9 glass was obvious.

  5. 比亚迪K9登陆武汉和曼谷

    Institute of Scientific and Technical Information of China (English)

    张艳

    2015-01-01

    3月1日,比亚迪绿色公交正式登陆武汉,2辆K9纯电动大巴走上武汉街头。作为全球首款全通道低地板纯电动公交,K9为武汉市民的绿色出行增添新动力。武汉K9纯电动大巴采用白绿色车身,简洁大方。首批2辆K9投放在武汉295路公交线路,从汉口火车站开往黄陂客运站,单车载客往返一次60个站点、约80公里,运营时间约3小时。

  6. TALE-directed local modulation of H3K9 methylation shapes exon recognition.

    Science.gov (United States)

    Bieberstein, Nicole I; Kozáková, Eva; Huranová, Martina; Thakur, Prasoon K; Krchňáková, Zuzana; Krausová, Michaela; Carrillo Oesterreich, Fernando; Staněk, David

    2016-07-21

    In search for the function of local chromatin environment on pre-mRNA processing we established a new tool, which allows for the modification of chromatin using a targeted approach. Using Transcription Activator-Like Effector domains fused to histone modifying enzymes (TALE-HME), we show locally restricted alteration of histone methylation modulates the splicing of target exons. We provide evidence that a local increase in H3K9 di- and trimethylation promotes inclusion of the target alternative exon, while demethylation by JMJD2D leads to exon skipping. We further demonstrate that H3K9me3 is localized on internal exons genome-wide suggesting a general role in splicing. Consistently, targeting of the H3K9 demethylase to a weak constitutive exon reduced co-transcriptional splicing. Together our data show H3K9 methylation within the gene body is a factor influencing recognition of both constitutive and alternative exons.

  7. Histone H3K9 methyltransferase G9a represses PPARγ expression and adipogenesis

    OpenAIRE

    Wang, Lifeng; Xu, Shiliyang; Lee, Ji-Eun; Baldridge, Anne; Grullon, Sean; Peng, Weiqun; Ge, Kai

    2012-01-01

    PPARγ promotes adipogenesis while Wnt proteins inhibit adipogenesis. However, the mechanisms that control expression of these positive and negative master regulators of adipogenesis remain incompletely understood. By genome-wide histone methylation profiling in preadipocytes, we find that among gene loci encoding adipogenesis regulators, histone methyltransferase (HMT) G9a-mediated repressive epigenetic mark H3K9me2 is selectively enriched on the entire PPARγ locus. H3K9me2 and G9a levels dec...

  8. Numerical simulation of temperature field in K9 glass irradiated by ultraviolet pulse laser

    Science.gov (United States)

    Wang, Xi; Fang, Xiaodong

    2015-10-01

    The optical component of photoelectric system was easy to be damaged by irradiation of high power pulse laser, so the effect of high power pulse laser irradiation on K9 glass was researched. A thermodynamic model of K9 glass irradiated by ultraviolet pulse laser was established using the finite element software ANSYS. The article analyzed some key problems in simulation process of ultraviolet pulse laser damage of K9 glass based on ANSYS from the finite element models foundation, meshing, loading of pulse laser, setting initial conditions and boundary conditions and setting the thermal physical parameters of material. The finite element method (FEM) model was established and a numerical analysis was performed to calculate temperature field in K9 glass irradiated by ultraviolet pulse laser. The simulation results showed that the temperature of irradiation area exceeded the melting point of K9 glass, while the incident laser energy was low. The thermal damage dominated in the damage mechanism of K9 glass, the melting phenomenon should be much more distinct.

  9. Data Conversion Between AutoCAD and MapGIS K9%AutoCAD与MapGIS K9数据转换

    Institute of Scientific and Technical Information of China (English)

    郑贵洲; 姚昳昕; 彭俊芳

    2013-01-01

    介绍了两种软件的数据特点和数据格式,阐述了AutoCAD和MapGIS K9块与子图、线型、颜色、图案、图层之间对照关系表的建立方法,详细概括了实现两种格式间数据转换的过程.

  10. Inhibition of H3K9 methyltransferase G9a ameliorates methylglyoxal-induced peritoneal fibrosis

    Science.gov (United States)

    Maeda, Kazuya; Doi, Shigehiro; Nakashima, Ayumu; Nagai, Takuo; Irifuku, Taisuke; Ueno, Toshinori; Masaki, Takao

    2017-01-01

    Activity of H3K9 histone methyltransferase G9a is reportedly induced by transforming growth factor-β1 (TGF-β1) and plays an important role in the progression of cancer and fibrosis. In this study, we investigated whether inhibition of G9a-mediated H3K9 methylation attenuates peritoneal fibrosis in mice and human peritoneal mesothelial cells (HPMCs). Nonadherent cells of peritoneal dialysis (PD) patients were isolated from PD effluent to examine expression of G9a. Peritoneal fibrosis was induced by peritoneal injection of methylglyoxal (MGO) in male C57/B6 mice for 3 weeks. BIX01294, a G9a inhibitor, was administered by subcutaneous injection. Effects of BIX01294 on MGO-induced pathological and functional changes in mice were evaluated by immunohistochemistry and a peritoneal equilibration test. HPMCs were isolated from human omentum, and the inhibitory effect of BIX01294 on TGF-β1-induced fibrotic changes was investigated in the HPMCs by western blotting. G9a was upregulated in nonadherent cells of human PD effluent, the peritoneum of MGO-injected mice, and TGF-β1-stimulated HPMCs. BIX01294 significantly reduced the submesothelial zone thickness and cell density in MGO-injected mice. Immunohistochemical staining revealed that BIX01294 treatment decreased not only mono-methylation of H3K9 (H3K9me1), but also the number of mesenchymal cells, accumulation of collagen, and infiltration of monocytes. In addition to the pathological changes, BIX01294 reduced the level of TGF-β1 in peritoneal fluid and improved peritoneal functions. Furthermore, BIX01294 inhibited TGF-β1-induced fibrotic changes along with suppression of H3K9me1 in HPMCs. Therefore, inhibition of H3K9 methyltransferase G9a suppresses peritoneal fibrosis through a reduction of H3K9me1. PMID:28278257

  11. Influence of ion beam bombardment on surface roughness of K9 glass substrate

    Science.gov (United States)

    Pan, Yongqiang; Huang, Guojun; Hang, Lingxia

    2010-10-01

    Ion beam bombardment optical substrate surface has become an important part of process of optical thin films deposition. In this work, the K9 optical glass is bombarded by the broad beam cold cathode ion source. The dependence of the K9 glass surface roughness on the ion beam bombardment time, the ion energy, the distance and incident angle are all investigated, respectively. Surface roughness of K9 glass is measured using Talysurf CCI. The experimental results show that when the ion energy is 800ev, the bombardment distance of 20cm, with the ion beam bombardment time increased, the K9 substrate surface roughness first increase and then decrease. When the ion beam bombardment distance is 20cm, bombardment time is 10min, with the bombardment energy increases, substrate surface roughness increase first and then decrease, especially in the ion energy greater than 1200ev, the optical substrate surface roughness rapidly increases. When the ion energy is 800 eV, bombardment time is 10min, with the bombardment distance increase, substrate surface roughness decrease gradually. Furthermore, the incident angle of ion beam plays an important role in improving the K9 glass surface roughness.

  12. 起亚旗舰K9正式发布

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    起亚目前发布了全新研发的旗舰豪华轿车——K9,新车将于2012年上半年在韩国上市销售。起亚表示K9也将在2012年年底销往韩国以外的市场,但尚未透露海外市场的车名和准确发布时间。全新K9是起亚旗下首款后驱轿车,将被作为起亚的旗舰车系替代2010年退市的欧菲莱斯(Opirus,又称为Amanti),但定位将有所提升。起亚表示K9拥有出类拔萃的设计和操控乐趣,并拥有大量先进技术,这表示我们可以将K9视为起亚最高研发和制造水平的展现。

  13. 浅析MapGIS K9的应用技术

    Institute of Scientific and Technical Information of China (English)

    李莉英

    2013-01-01

    MapGIS K9是采用新一代GIs架构技术和开发模式,面向网络超大型分布式地理信息系统基础软件平台.MapGIS K9中地质制图是一项重要的组成部分,文章简述MapGIS K9在地质制图中的应用技术,以便为地质制图人员提供参考.地质制图是一项较为复杂的综合性工作,它需要制图人员在大量地质调查的基础上,运用先进技术完成制图工作.

  14. 起亚旗舰车型K9韩国上市

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    日前,现代起亚汽车全新旗舰车型K9正式在韩国上市销售。全新起亚K9豪华轿车与现代雅科仕共享技术平台,它出自前奥迪设计师彼得·希瑞尔之手,外形更加优雅、时尚。全新起亚K9装备了3.8L排量V6汽油发动机,最高输出功率210kW。

  15. File list: His.PSC.50.H3K9K14ac.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.50.H3K9K14ac.AllCell mm9 Histone H3K9K14ac Pluripotent stem cell ERX245530,...1 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.50.H3K9K14ac.AllCell.bed ...

  16. File list: His.PSC.20.H3K9ac.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.20.H3K9ac.AllCell hg19 Histone H3K9ac Pluripotent stem cell SRX067524,SRX03...8537,SRX951984,SRX951983,SRX038538,SRX095381 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.PSC.20.H3K9ac.AllCell.bed ...

  17. Study on fine annealing process of the large-aperture K9 glasses

    Science.gov (United States)

    Gang, Wang; Bin, Liu Yi; Zheng, Li Li; Hui, Zhang; Lei, Xie; Min, Qiu Fu; Ping, Ma; Yao, Yan Ding

    2016-10-01

    Study on fine annealing process of the large-aperture K9 glasses was carried out in the report. The process parameters of glass placed way, fan speed and design of the cavity for keeping temperature uniformity were attained. By the fine annealing experiment, the stress distribution was improved evidently. The stress changed from Irregular distribution to consistency symmetric distribution and the stress max was reduced. The surface profile accuracy of the large-aperture K9 glasses was controlled steadily during CNC polishing.

  18. 鲁科版高中化学教科书STS教育内容分析%Analysis on the STS Education Contents of High School Chemistry Textbooks of Shandong Version

    Institute of Scientific and Technical Information of China (English)

    王建芬; 陈彦芬

    2012-01-01

    STS education reflects the educational philosophy of the new high school chemistry curriculum reform which aims to improve students' scientific literacy.It lays emphasis on the relations between science,technology and society,and can help students analyze and solve chemical problems in a correct way.The high school chemistry textbooks of Shandong version focus on the construction of chemical knowledge in the context of human life,and select rich contents of STS education.The characteristics of organization and presentation are presentation by chapter headings and column system and integration of STS contents knowledge with element compounds.%STS教育体现了高中化学新课程改革全面提升学生科学素养的教育理念,重视科学、技术与社会相互联系,有助于学生正确地分析、解决化学问题.鲁科版高中化学教科书注重在人类生活背景下建构化学知识,选取了丰富的STS教育内容,其组织和呈现方式特点为:利用教材的章节标题呈现;利用教材栏目体系呈现;STS内容与元素化合物知识相互融合渗透.

  19. K9 Buddies: A Program of Guide Dogs for the Blind

    Science.gov (United States)

    Ritter, Joanne

    2007-01-01

    Today, exceptional dogs that have been specially bred and socialized are paired with children who are blind or visually impaired. These dogs, called "K9 Buddies," are from Guide Dogs for the Blind, a national nonprofit organization with a mission to offer skilled mobility dogs and training free-of-charge to adults with visual impairments…

  20. The H3K9 dimethyltransferases EHMT1/2 protect against pathological cardiac hypertrophy

    Science.gov (United States)

    Aronsen, Jan Magnus; Ferrini, Arianna; Brien, Patrick; Alkass, Kanar; Tomasso, Antonio; Agrawal, Asmita; Bergmann, Olaf; Reik, Wolf; Roderick, Hywel Llewelyn

    2016-01-01

    Cardiac hypertrophic growth in response to pathological cues is associated with reexpression of fetal genes and decreased cardiac function and is often a precursor to heart failure. In contrast, physiologically induced hypertrophy is adaptive, resulting in improved cardiac function. The processes that selectively induce these hypertrophic states are poorly understood. Here, we have profiled 2 repressive epigenetic marks, H3K9me2 and H3K27me3, which are involved in stable cellular differentiation, specifically in cardiomyocytes from physiologically and pathologically hypertrophied rat hearts, and correlated these marks with their associated transcriptomes. This analysis revealed the pervasive loss of euchromatic H3K9me2 as a conserved feature of pathological hypertrophy that was associated with reexpression of fetal genes. In hypertrophy, H3K9me2 was reduced following a miR-217–mediated decrease in expression of the H3K9 dimethyltransferases EHMT1 and EHMT2 (EHMT1/2). miR-217–mediated, genetic, or pharmacological inactivation of EHMT1/2 was sufficient to promote pathological hypertrophy and fetal gene reexpression, while suppression of this pathway protected against pathological hypertrophy both in vitro and in mice. Thus, we have established a conserved mechanism involving a departure of the cardiomyocyte epigenome from its adult cellular identity to a reprogrammed state that is accompanied by reexpression of fetal genes and pathological hypertrophy. These results suggest that targeting miR-217 and EHMT1/2 to prevent H3K9 methylation loss is a viable therapeutic approach for the treatment of heart disease. PMID:27893464

  1. Differential localization and independent acquisition of the H3K9me2 and H3K9me3 chromatin modifications in the Caenorhabditis elegans adult germ line.

    Directory of Open Access Journals (Sweden)

    Jessica B Bessler

    2010-01-01

    Full Text Available Histone methylation is a prominent feature of eukaryotic chromatin that modulates multiple aspects of chromosome function. Methyl modification can occur on several different amino acid residues and in distinct mono-, di-, and tri-methyl states. However, the interplay among these distinct modification states is not well understood. Here we investigate the relationships between dimethyl and trimethyl modifications on lysine 9 of histone H3 (H3K9me2 and H3K9me3 in the adult Caenorhabditis elegans germ line. Simultaneous immunofluorescence reveals very different temporal/spatial localization patterns for H3K9me2 and H3K9me3. While H3K9me2 is enriched on unpaired sex chromosomes and undergoes dynamic changes as germ cells progress through meiotic prophase, we demonstrate here that H3K9me3 is not enriched on unpaired sex chromosomes and localizes to all chromosomes in all germ cells in adult hermaphrodites and until the primary spermatocyte stage in males. Moreover, high-copy transgene arrays carrying somatic-cell specific promoters are highly enriched for H3K9me3 (but not H3K9me2 and correlate with DAPI-faint chromatin domains. We further demonstrate that the H3K9me2 and H3K9me3 marks are acquired independently. MET-2, a member of the SETDB histone methyltransferase (HMTase family, is required for all detectable germline H3K9me2 but is dispensable for H3K9me3 in adult germ cells. Conversely, we show that the HMTase MES-2, an E(z homolog responsible for H3K27 methylation in adult germ cells, is required for much of the germline H3K9me3 but is dispensable for H3K9me2. Phenotypic analysis of met-2 mutants indicates that MET-2 is nonessential for fertility but inhibits ectopic germ cell proliferation and contributes to the fidelity of chromosome inheritance. Our demonstration of the differential localization and independent acquisition of H3K9me2 and H3K9me3 implies that the trimethyl modification of H3K9 is not built upon the dimethyl modification in

  2. Cardiotoxicity associated with the synthetic cannabinoid, K9, with laboratory confirmation.

    Science.gov (United States)

    Young, Amy C; Schwarz, Evan; Medina, Genevieve; Obafemi, Adebisi; Feng, Sing-Yi; Kane, Colin; Kleinschmidt, Kurt

    2012-09-01

    Synthetic cannabinoids have been popular recreational drugs of abuse for their psychoactive properties. Five of the many synthetic cannabinoids have been recently banned in the United States because of their unknown and potentially harmful adverse effects. Little is known about these substances. They are thought to have natural cannabinoid-like effects but have different chemical structures. Adverse effects related to synthetic cannabinoids are not well known. We provide clinical effects and patient outcome following K9 use. In addition, we briefly review synthetic cannabinoids. We present a 17-year-old adolescent boy with chest pain, tachycardia, and then bradycardia associated with smoking K9. Two synthetic cannabinoids, JWH-018 and JWH-073, were confirmed on laboratory analysis. In addition to the limited current data, we demonstrate harmful adverse effects related to toxicity of 2 synthetic cannabinoids. Further studies are needed.

  3. 商务大屏 双网双待 中恒K9

    Institute of Scientific and Technical Information of China (English)

    GWY

    2012-01-01

    一直主推商务手机的中恒,在功能上采用逐步升级的策略,以此满足不同层次用户的需要。K9从型号上来看是K8的延续,两者均强调双网双待,后者支持GSM/WCDMA,后者则支持GSM/CDMA2000。因此从功能上来看,K9也可以看作是SX30+的大屏升级版。

  4. Heterochromatic genome stability requires regulators of histone H3 K9 methylation.

    Directory of Open Access Journals (Sweden)

    Jamy C Peng

    2009-03-01

    Full Text Available Heterochromatin contains many repetitive DNA elements and few protein-encoding genes, yet it is essential for chromosome organization and inheritance. Here, we show that Drosophila that lack the Su(var3-9 H3K9 methyltransferase display significantly elevated frequencies of spontaneous DNA damage in heterochromatin, in both somatic and germ-line cells. Accumulated DNA damage in these mutants correlates with chromosomal defects, such as translocations and loss of heterozygosity. DNA repair and mitotic checkpoints are also activated in mutant animals and are required for their viability. Similar effects of lower magnitude were observed in animals that lack the RNA interference pathway component Dcr2. These results suggest that the H3K9 methylation and RNAi pathways ensure heterochromatin stability.

  5. Heterochromatic genome stability requires regulators of histone H3 K9 methylation.

    Science.gov (United States)

    Peng, Jamy C; Karpen, Gary H

    2009-03-01

    Heterochromatin contains many repetitive DNA elements and few protein-encoding genes, yet it is essential for chromosome organization and inheritance. Here, we show that Drosophila that lack the Su(var)3-9 H3K9 methyltransferase display significantly elevated frequencies of spontaneous DNA damage in heterochromatin, in both somatic and germ-line cells. Accumulated DNA damage in these mutants correlates with chromosomal defects, such as translocations and loss of heterozygosity. DNA repair and mitotic checkpoints are also activated in mutant animals and are required for their viability. Similar effects of lower magnitude were observed in animals that lack the RNA interference pathway component Dcr2. These results suggest that the H3K9 methylation and RNAi pathways ensure heterochromatin stability.

  6. Acupuncture promotes angiogenesis after myocardial ischemia through H3K9 acetylation regulation at VEGF gene.

    Directory of Open Access Journals (Sweden)

    Shu-Ping Fu

    Full Text Available BACKGROUND: Acupuncture exerts cardioprotective effects on several types of cardiac injuries, especially myocardial ischemia (MI, but the mechanisms have not yet been well elucidated. Angiogenesis mediated by VEGF gene expression and its modification through histone acetylation has been considered a target in treating myocardial ischemia. This study aims to exam whether modulation of angiogenesis through H3K9 acetylation regulation at VEGF gene is one possible cardioprotective mechanism of acupuncture. RESULTS: We generated rat MI models by ligating the left anterior descending coronary artery and applied electroacupuncture (EA treatment at the Neiguan (PC6 acupoint. Our results showed that acupuncture reversed the S-T segment change, reduced Q-wave area, decreased CK, CK-MB, LDH levels, mitigated myocardial remodeling, and promoted microvessel formation in the MI heart. RNA-seq analysis showed that VEGF-induced angiogenesis signaling was involved in the modulation of EA. Western blot results verified that the protein expressions of VEGF, Ras, phospho-p44/42 MAPK, phospho-p38 MAPK, phospho-SAPK/JNK and Akt, were all elevated significantly by EA treatment in the MI heart. Furthermore, increased H3K9 acetylation was also observed according with the VEGF. ChIP assay confirmed that EA treatment could notably stimulate the recruitment of H3K9ace at the VEGF promoter. CONCLUSIONS: Our study demonstrates for the first time that acupuncture can effectively up-regulate VEGF expression through H3K9 acetylation modification directly at the VEGF promoter and hence activate VEGF-induced angiogenesis in rat MI models. We employed high throughput sequencing in this study and, for the first time, generated genome-wide gene expression profiles both in the rat MI model and in acupuncture treatment.

  7. Acupuncture promotes angiogenesis after myocardial ischemia through H3K9 acetylation regulation at VEGF gene.

    Science.gov (United States)

    Fu, Shu-Ping; He, Su-Yun; Xu, Bin; Hu, Chen-Jun; Lu, Sheng-Feng; Shen, Wei-Xing; Huang, Yan; Hong, Hao; Li, Qian; Wang, Ning; Liu, Xuan-Liang; Liang, Fanrong; Zhu, Bing-Mei

    2014-01-01

    Acupuncture exerts cardioprotective effects on several types of cardiac injuries, especially myocardial ischemia (MI), but the mechanisms have not yet been well elucidated. Angiogenesis mediated by VEGF gene expression and its modification through histone acetylation has been considered a target in treating myocardial ischemia. This study aims to exam whether modulation of angiogenesis through H3K9 acetylation regulation at VEGF gene is one possible cardioprotective mechanism of acupuncture. We generated rat MI models by ligating the left anterior descending coronary artery and applied electroacupuncture (EA) treatment at the Neiguan (PC6) acupoint. Our results showed that acupuncture reversed the S-T segment change, reduced Q-wave area, decreased CK, CK-MB, LDH levels, mitigated myocardial remodeling, and promoted microvessel formation in the MI heart. RNA-seq analysis showed that VEGF-induced angiogenesis signaling was involved in the modulation of EA. Western blot results verified that the protein expressions of VEGF, Ras, phospho-p44/42 MAPK, phospho-p38 MAPK, phospho-SAPK/JNK and Akt, were all elevated significantly by EA treatment in the MI heart. Furthermore, increased H3K9 acetylation was also observed according with the VEGF. ChIP assay confirmed that EA treatment could notably stimulate the recruitment of H3K9ace at the VEGF promoter. Our study demonstrates for the first time that acupuncture can effectively up-regulate VEGF expression through H3K9 acetylation modification directly at the VEGF promoter and hence activate VEGF-induced angiogenesis in rat MI models. We employed high throughput sequencing in this study and, for the first time, generated genome-wide gene expression profiles both in the rat MI model and in acupuncture treatment.

  8. Validation and Development of a Certification Program for Using K9s to Survey Desert Tortoises

    Science.gov (United States)

    2013-08-01

    Esque, and K. Nussear. Humans vs. K9s: Is fear warranted in the race to save the desert tortoise? 91st Annual Ecological Society of America , August 6-11...desert tortoises, other reptiles (Schwartz et al., 1984; Engeman et al., 1998), or even to 10 distinguish cancer in human subjects (McCulloch et...has yet to be validated in mammals. Therefore, it is not possible to explain precisely how dogs use scent to find desert tortoises, other reptiles

  9. Repeat Composition of CenH3-chromatin and H3K9me2-marked heterochromatin in Sugar Beet (Beta vulgaris).

    Science.gov (United States)

    Kowar, Teresa; Zakrzewski, Falk; Macas, Jiří; Kobližková, Andrea; Viehoever, Prisca; Weisshaar, Bernd; Schmidt, Thomas

    2016-05-26

    Sugar beet (Beta vulgaris) is an important crop of temperate climate zones, which provides nearly 30 % of the world's annual sugar needs. From the total genome size of 758 Mb, only 567 Mb were incorporated in the recently published genome sequence, due to the fact that regions with high repetitive DNA contents (e.g. satellite DNAs) are only partially included. Therefore, to fill these gaps and to gain information about the repeat composition of centromeres and heterochromatic regions, we performed chromatin immunoprecipitation followed by sequencing (ChIP-Seq) using antibodies against the centromere-specific histone H3 variant of sugar beet (CenH3) and the heterochromatic mark of dimethylated lysine 9 of histone H3 (H3K9me2). ChIP-Seq analysis revealed that active centromeres containing CenH3 consist of the satellite pBV and the Ty3-gypsy retrotransposon Beetle7, while heterochromatin marked by H3K9me2 exhibits heterogeneity in repeat composition. H3K9me2 was mainly associated with the satellite family pEV, the Ty1-copia retrotransposon family Cotzilla and the DNA transposon superfamily of the En/Spm type. In members of the section Beta within the genus Beta, immunostaining using the CenH3 antibody was successful, indicating that orthologous CenH3 proteins are present in closely related species within this section. The identification of repetitive genome portions by ChIP-Seq experiments complemented the sugar beet reference sequence by providing insights into the repeat composition of poorly characterized CenH3-chromatin and H3K9me2-heterochromatin. Therefore, our work provides the basis for future research and application concerning the sugar beet centromere and repeat-rich heterochromatic regions characterized by the presence of H3K9me2.

  10. Study on Dynamic Compression Properties of K9 Glass with Doppler Pins Array Measurements

    Science.gov (United States)

    Changming, Hu; Xiang, Wang; Lingcang, Cai; Cangli, Liu

    2009-06-01

    K9 glass is one of archetypal brittle materials for studies of dynamic fracture, failure wave, and so on. This paper presented the dynamic compression properties of K9 glass under uniaxial strain condition. Experimental sample is K9 glass with internal pre-existed defects, and the shape of pre-existed defects is disc with less than 0.5 mm diameter. All tests were conducted by power gun with 37 mm diameter chamber. Doppler Pins array with high space-time resolutions, which consists of sixteen pins in range of 2 mm line length, were applied to measure the particle velocity histories in different positions at the sample rear surface, and the space-resolution is 127 μm, Experimental results show failure waves initiate at internal micro-surfaces of the sample under shock loading, and the dynamic stress concentration is likely attributed to be a physical mechanism of the initiation of the failure wave. These defects that by the controlled laser irradiation in advance are some internal micro-surfaces. Meanwhile, the experimental results show that internal micro-surfaces of the sample have influence on the elastic precursor wave decay.

  11. ESET/SETDB1 gene expression and histone H3 (K9) trimethylation in Huntington's disease.

    Science.gov (United States)

    Ryu, Hoon; Lee, Junghee; Hagerty, Sean W; Soh, Byoung Yul; McAlpin, Sara E; Cormier, Kerry A; Smith, Karen M; Ferrante, Robert J

    2006-12-12

    Chromatin remodeling and transcription regulation are tightly controlled under physiological conditions. It has been suggested that altered chromatin modulation and transcription dysfunction may play a role in the pathogenesis of Huntington's disease (HD). Increased histone methylation, a well established mechanism of gene silencing, results in transcriptional repression. ERG-associated protein with SET domain (ESET), a histone H3 (K9) methyltransferase, mediates histone methylation. We show that ESET expression is markedly increased in HD patients and in transgenic R6/2 HD mice. Similarly, the protein level of trimethylated histone H3 (K9) was also elevated in HD patients and in R6/2 mice. We further demonstrate that both specificity protein 1 (Sp1) and specificity protein 3 (Sp3) act as transcriptional activators of the ESET promoter in neurons and that mithramycin, a clinically approved guanosine-cytosine-rich DNA binding antitumor antibiotic, interferes with the DNA binding of these Sp family transcription factors, suppressing basal ESET promoter activity in a dose dependent manner. The combined pharmacological treatment with mithramycin and cystamine down-regulates ESET gene expression and reduces hypertrimethylation of histone H3 (K9). This polytherapy significantly ameliorated the behavioral and neuropathological phenotype in the R6/2 mice and extended survival over 40%, well beyond any existing reported treatment in HD mice. Our data suggest that modulation of gene silencing mechanisms, through regulation of the ESET gene is important to neuronal survival and, as such, may be a promising treatment in HD patients.

  12. Optical properties of K9 glass waveguides fabricated by using carbon-ion implantation

    Science.gov (United States)

    Liu, Chun-Xiao; Wei, Wei; Fu, Li-Li; Zhu, Xu-Feng; Guo, Hai-Tao; Li, Wei-Nan; Lin, She-Bao

    2016-07-01

    K9 glass is a material with promising properties that make it attractive for optical devices. Ion implantation is a powerful technique to form waveguides with controllable depth and refractive index profile. In this work, optical planar waveguide structures were fabricated in K9 glasses by using 6.0-MeV C3+-ion implantation with a fluence of 1.0 × 1015 ions/cm2. The effective refractive indices of the guided modes were measured by using a prism-coupling system. The refractive index change in the ion-irradiated region was simulated by using the intensity calculation method. The modal intensity profile of the waveguide was calculated and measured by using the finite difference beam propagation method and the end-face coupling technique, respectively. The transmission spectra before and after the implantation showed that the main absorption band was not influenced by the low fluence dopants. The optical properties of the carbon-implanted K9 glass waveguides show promise for use as integrated photonic devices.

  13. File list: His.PSC.20.H3K9ac.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.20.H3K9ac.AllCell mm9 Histone H3K9ac Pluripotent stem cell SRX185844,SRX185...848,SRX873351,SRX873349,SRX873345,SRX097641,SRX130021,SRX873347,SRX187620 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.20.H3K9ac.AllCell.bed ...

  14. Histone H3-K9 methyltransferase ESET is essential for early development.

    Science.gov (United States)

    Dodge, Jonathan E; Kang, Yong-Kook; Beppu, Hideyuki; Lei, Hong; Li, En

    2004-03-01

    Methylation of histone H3 at lysine 9 (H3-K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing gene expression at euchromatic sites. ESET, G9a, SUV39-h1, SUV39-h2, and Eu-HMTase are histone methyltransferases that catalyze H3-K9 methylation in mammalian cells. Previous studies demonstrate that the SUV39-h proteins are preferentially targeted to the pericentric heterochromatin, and mice lacking both Suv39-h genes show cytogenetic abnormalities and an increased incidence of lymphoma. G9a methylates H3-K9 in euchromatin, and G9a null embryos die at 8.5 days postcoitum (dpc). G9a null embryo stem (ES) cells show altered DNA methylation in the Prader-Willi imprinted region and ectopic expression of the Mage genes. So far, an Eu-HMTase mouse knockout has not been reported. ESET catalyzes methylation of H3-K9 and localizes mainly in euchromatin. To investigate the in vivo function of Eset, we have generated an allele that lacks the entire pre- and post-SET domains and that expresses lacZ under the endogenous regulation of the Eset gene. We found that zygotic Eset expression begins at the blastocyst stage and is ubiquitous during postimplantation mouse development, while the maternal Eset transcripts are present in oocytes and persist throughout preimplantation development. The homozygous mutations of Eset resulted in peri-implantation lethality between 3.5 and 5.5 dpc. Blastocysts null for Eset were recovered but in less than Mendelian ratios. Upon culturing, 18 of 24 Eset(-/-) blastocysts showed defective growth of the inner cell mass and, in contrast to the approximately 65% recovery of wild-type and Eset(+/-) ES cells, no Eset(-/-) ES cell lines were obtained. Global H3-K9 trimethylation and DNA methylation at IAP repeats in Eset(-/-) blastocyst outgrowths were not dramatically altered. Together, these results suggest that Eset is required for peri-implantation development and the survival of ES cells.

  15. DNA methylation and histone H3-K9 modifications contribute to MUC17 expression.

    Science.gov (United States)

    Kitamoto, Sho; Yamada, Norishige; Yokoyama, Seiya; Houjou, Izumi; Higashi, Michiyo; Goto, Masamichi; Batra, Surinder K; Yonezawa, Suguru

    2011-02-01

    MUC17 glycoprotein is a membrane-associated mucin that is mainly expressed in the digestive tract. It has been suggested that MUC17 expression is correlated with the malignancy potential of pancreatic ductal adenocarcinomas (PDACs). In the present study, we provided the first report of the MUC17 gene expression through epigenetic regulation such as promoter methylation, histone modification and microRNA (miRNA) expression. Near the transcriptional start site, the DNA methylation level of MUC17-negative cancer cell lines (e.g. PANC1) was high, whereas that of MUC17-positive cells (e.g. AsPC-1) was low. Histone H3-K9 (H3-K9) modification status was also closely related to MUC17 expression. Our results indicate that DNA methylation and histone H3-K9 modification in the 5' flanking region play a critical role in MUC17 expression. Furthermore, the hypomethylation status was observed in patients with PDAC. This indicates that the hypomethylation status in the MUC17 promoter could be a novel epigenetic marker for the diagnosis of PDAC. In addition, the result of miRNA microarray analysis showed that five potential miRNA candidates existed. It is also possible that the MUC17 might be post-transcriptionally regulated by miRNA targeting to the 3'-untranslated region of its mRNA. These understandings of the epigenetic changes of MUC17 may be of importance for the diagnosis of carcinogenic risk and the prediction of outcomes for cancer patients.

  16. Study on dynamic compression performance of K9 glass with prefabricated defects

    Science.gov (United States)

    Hu, Changming; Wang, Xiang; Cai, Lingcang; Liu, Cangli

    2012-03-01

    We conducted several planar impact experiments to study dynamic compression properties of K9 glass on powder gun using Photon Doppler Velocimetry (PDV) measure system. Samples are prefabricated some internal meso-defects by laser three-dimensional erosion technique before shock loading. Free surface velocities recorded by high temporal-spatial resolution PDV array or multi-point PDV. All these experimental results show some different properties influenced by evolutions of pre-existed internal defects face on free surface velocity profiles. The critical compression strength and dynamic evolution information of pre-existed internal defects can be derived from the experimental results tentatively.

  17. Fast Predicting Statistical Subsurface Damage Parameters of the K9 Sample

    Science.gov (United States)

    Wang, Hairong; Chen, Hongfeng; Xiao, Lihui; Zhang, Bike; Jiang, Zhuangde

    2015-07-01

    Based on the subsurface damage model and the material removal rate of K9 glass in HF acid solution, a fast method is proposed to calculate the parameters of characterizing the subsurface damage of a polished sample. When micro cracks of the etched sample's subsurface can be clearly observed, lengths, widths, angles, densities of the micro cracks can be calculated by using the image processing algorithm, and depths of the micro cracks may be predicted by the load-crack model. Eventually a set of the parameters are proposed as a complete description about subsurface damage of the sample.

  18. Evaluación cardiovascular en perros de atención inmediata (k9)

    OpenAIRE

    Julio Adrián Benítez Tezoquipa; Roberto Villa Ocaranza; Alejandro Rodolfo Reynoso Palomar

    2012-01-01

    El estudio de las respuestas al ejercicio y entrenamiento tipo K9 en perros, ha sido poco evaluado a diferencia de otros tipos de entrenamiento en deportes o utilidades en canidos. El ejercicio continuo y el entrenamiento exhaustivo conducen al desgaste físico y emocional de los perros, por lo que realizar la valoración del sistema cardiovascular permitió determinar el tipo, características y posibles factores involucrados en el desarrollo de cardiopatías en este tipo de animales. Se efectuar...

  19. A histone H3K9M mutation traps histone methyltransferase Clr4 to prevent heterochromatin spreading

    Energy Technology Data Exchange (ETDEWEB)

    Shan, Chun-Min; Wang, Jiyong; Xu, Ke; Chen, Huijie; Yue, Jia-Xing; Andrews, Stuart; Moresco, James J.; Yates, John R.; Nagy, Peter L.; Tong, Liang; Jia, Songtao

    2016-09-20

    Histone lysine-to-methionine (K-to-M) mutations are associated with multiple cancers, and they function in a dominant fashion to block the methylation of corresponding lysines on wild type histones. However, their mechanisms of function are controversial. Here we show that in fission yeast, introducing the K9M mutation into one of the three histone H3 genes dominantly blocks H3K9 methylation on wild type H3 across the genome. In addition, H3K9M enhances the interaction of histone H3 tail with the H3K9 methyltransferase Clr4 in a SAM (S-adenosyl-methionine)-dependent manner, and Clr4 is trapped at nucleation sites to prevent its spreading and the formation of large heterochromatin domains. We further determined the crystal structure of an H3K9M peptide in complex with human H3K9 methyltransferase G9a and SAM, which reveales that the methionine side chain had enhanced van der Waals interactions with G9a. Therefore, our results provide a detailed mechanism by which H3K9M regulates H3K9 methylation.

  20. 固结磨料化学机械研抛K9玻璃的材料去除机理%Material Removal Mechanism of K9 Glass During Chemical Mechanical Polishing by Fixed Abrasive Pad

    Institute of Scientific and Technical Information of China (English)

    樊吉龙; 朱永伟; 李军; 叶剑锋

    2012-01-01

    采用失重法对固结磨料研抛K9玻璃材料去除过程中的机械与化学作用进行了分离,采用显微硬度方法分析了研抛液对K9玻璃工件表层硬度的影响.研究结果表明:研抛液能与K9玻璃发生化学反应并在其表面形成一层较基质材料软的变质层,变质层厚度随浸泡时间的延长而增加;单纯研抛液的化学作用对K9玻璃的材料去除作用有限,固结磨料研抛垫对K9玻璃的机械去除作用主要以脆性去除为主,化学与机械的交互作用是K9玻璃影响材料去除的主要方式:当研抛盘转速为200r/min时,化学作用与机械作用达到平衡,交互作用最为强烈,材料去除率达到最大值.%The mechanical and chemical functions during the material removal process are separated by adopting weight loss measurements when fixed abrasive pad works on K9 glass. The influence of polishing slurry on the surface hardness of K9 glass is also analyzed by the micro-hardness method. The research results show that the chemical reaction between polishing slurry and K9 glass will take place and form a denatured layer on the surface of K9 glass, which is softer than host material and whose thickness increases with soak time. The material removal rate of K9 glass by chemical function is very limited and the mechanical function of K9 glass is mainly by brittle removal, while the interaction of chemical and mechanical functions is the main factor in influencing the material removal rate of K9 glass. When the rotation speed of the polishing pad is 200 r/min, the chemical and mechanical functions come to a balance with their interaction being the strongest, and the material removal rate reaches its maximum.

  1. The tumor suppressor, parafibromin, mediates histone H3 K9 methylation for cyclin D1 repression.

    Science.gov (United States)

    Yang, Yong-Jin; Han, Jeung-Whan; Youn, Hong-Duk; Cho, Eun-Jung

    2010-01-01

    Parafibromin, a component of the RNA polymerase II-associated PAF1 complex, is a tumor suppressor linked to hyperparathyroidism-jaw tumor syndrome and sporadic parathyroid carcinoma. Parafibromin induces cell cycle arrest by repressing cyclin D1 via an unknown mechanism. Here, we show that parafibromin interacts with the histone methyltransferase, SUV39H1, and functions as a transcriptional repressor. The central region (128-227 amino acids) of parafibromin is important for both the interaction with SUV39H1 and transcriptional repression. Parafibromin associated with the promoter and coding regions of cyclin D1 and was required for the recruitment of SUV39H1 and the induction of H3 K9 methylation but not H3 K4 methylation. RNA interference analysis showed that SUV39H1 was critical for cyclin D1 repression. These data suggest that parafibromin plays an unexpected role as a repressor in addition to its widely known activity associated with transcriptional activation. Parafibromin as a part of the PAF1 complex might downregulate cyclin D1 expression by integrating repressive H3 K9 methylation during transcription.

  2. K9玻璃的离子束抛光

    Institute of Scientific and Technical Information of China (English)

    苏志伟; 陈庆川

    2005-01-01

    K9玻璃是常用的光学和微系统材料。随着光学和微系统技术的迅速发展,由于具有较低廉的价格、良好的微加工性能,在K9玻璃上制作微米、纳米尺度的微结构和微器件的技术,已应用于光电子、微波技术、衍射光学元件等众多领域。但是,由于光学和微波等器件的表面粗糙可引起光散射和信号损失,从而降低器件性能。因此材料表面的超光滑处理对提高光学和微波等器件的性能具有十分重要的意义。

  3. MapGIS K9软件在数字地质图中的应用%DISCUSSION ON THE APPLICATION OF MAPGIS K9 SOFTWARE IN DIGITAL GEOLOGICAL MAPPING

    Institute of Scientific and Technical Information of China (English)

    代丽霞; 胡红霞; 朱忠梅

    2013-01-01

    从制图的角度,结合工作实践阐述了MapGIS K9在地形地质图的绘制、图形数据库的建立等方面的应用.使制图专业人员对MapGIS K9软件更加了解,并对其基本功能与应用由感性上升到理性认识,从而更好地掌握MapGIS K9,提高制图质量及精度要求.

  4. Dissolution behavior of bioactive glass ceramics with different CaO/MgO ratios in SBF-K9 and r-SBF$

    Institute of Scientific and Technical Information of China (English)

    M.U. Hashmi; Saqlain A. Shah

    2014-01-01

    In the present work, we studied dissolution behavior of three glass ceramics samples each having 34 SiO2-14.5 P2O5-1 CaF2-0.5 MgF2 (%wt) and ratio of CaO/MgO varying from 11.5:1 to 1:11.5 in conventional SBF (SBF-K9) and revised SBF (r-SBF) that has ionic concentration exactly equal to that of human blood plasma. For that purpose, samples were immersed in fluids for different time periods upto 25 days. Thin film XRD analysis revealed the diffusive nature of the phases on the surfaces of samples after soaking for different time periods in r-SBF. It showed the poor precipitation and small thickness of the HCAp layer on the samples as compared to that in SBF-K9, thus indicating the fitness and sensitivity of r-SBF for in-vitro characterization of samples. AAS, FTIR and EDS revealed slow bonding rate on the surfaces of the samples in r-SBF than that in SBF-K9 that showed the dependence of bond formation on the composition of the materials as well as on the physiological fluid used for in-vitro characterization. The rate of HCAp formation was slower in r-SBF due to more competitive adsorption of CO3- ions to Ca and Mg ions owing to greater amount of CO3- in r-SBF than that in SBF-K9. It shows the importance of CO3- content in the physiological fluids for the in-vitro assessment of samples. So, r-SBF is recommended to be used for assessment of samples to clearly understand their behavior in-vivo.

  5. Dissolution behavior of bioactive glass ceramics with different CaO/MgO ratios in SBF-K9 and r-SBF

    Directory of Open Access Journals (Sweden)

    M.U. Hashmi

    2014-08-01

    Full Text Available In the present work, we studied dissolution behavior of three glass ceramics samples each having 34 SiO2–14.5 P2O5–1 CaF2–0.5 MgF2 (%wt and ratio of CaO/MgO varying from 11.5:1 to 1:11.5 in conventional SBF (SBF-K9 and revised SBF (r-SBF that has ionic concentration exactly equal to that of human blood plasma. For that purpose, samples were immersed in fluids for different time periods upto 25 days. Thin film XRD analysis revealed the diffusive nature of the phases on the surfaces of samples after soaking for different time periods in r-SBF. It showed the poor precipitation and small thickness of the HCAp layer on the samples as compared to that in SBF-K9, thus indicating the fitness and sensitivity of r-SBF for in-vitro characterization of samples. AAS, FTIR and EDS revealed slow bonding rate on the surfaces of the samples in r-SBF than that in SBF-K9 that showed the dependence of bond formation on the composition of the materials as well as on the physiological fluid used for in-vitro characterization. The rate of HCAp formation was slower in r-SBF due to more competitive adsorption of CO3− ions to Ca and Mg ions owing to greater amount of CO3− in r-SBF than that in SBF-K9. It shows the importance of CO3− content in the physiological fluids for the in-vitro assessment of samples. So, r-SBF is recommended to be used for assessment of samples to clearly understand their behavior in-vivo.

  6. HP1a recruitment to promoters is independent of H3K9 methylation in Drosophila melanogaster.

    Directory of Open Access Journals (Sweden)

    Margarida L A Figueiredo

    Full Text Available Heterochromatin protein 1 (HP1 proteins, recognized readers of the heterochromatin mark methylation of histone H3 lysine 9 (H3K9me, are important regulators of heterochromatin-mediated gene silencing and chromosome structure. In Drosophila melanogaster three histone lysine methyl transferases (HKMTs are associated with the methylation of H3K9: Su(var3-9, Setdb1, and G9a. To probe the dependence of HP1a binding on H3K9me, its dependence on these three HKMTs, and the division of labor between the HKMTs, we have examined correlations between HP1a binding and H3K9me patterns in wild type and null mutants of these HKMTs. We show here that Su(var3-9 controls H3K9me-dependent binding of HP1a in pericentromeric regions, while Setdb1 controls it in cytological region 2L:31 and (together with POF in chromosome 4. HP1a binds to the promoters and within bodies of active genes in these three regions. More importantly, however, HP1a binding at promoters of active genes is independent of H3K9me and POF. Rather, it is associated with heterochromatin protein 2 (HP2 and open chromatin. Our results support a hypothesis in which HP1a nucleates with high affinity independently of H3K9me in promoters of active genes and then spreads via H3K9 methylation and transient looping contacts with those H3K9me target sites.

  7. The plasma spectrum research on K9 glass under nanosecond pulse laser%ns脉冲激光作用下K9玻璃的等离子体谱图研究

    Institute of Scientific and Technical Information of China (English)

    刘丽炜; 蔡红星; 李昌立; 何双力; 张喜和; 冯锴

    2008-01-01

    建立一套激光等离子体光谱测量实验装置,以Nd:YAG调Q倍频固体激光器为激发光源,作用K9玻璃.通过对K9的等离子体谱图的分析发现,K9玻璃等离子体谱强度的变化与激光器输出能量呈线性关系.同时利用显微镜观察K9玻璃的表面损伤形貌,并进行理论分析.

  8. Research and Application of Flex Development Mode Based on MapGIS K9 IMS%MapGIS K9 IMS的Flex开发模式研究及应用

    Institute of Scientific and Technical Information of China (English)

    秦超; 姜良君; 薛逸飞; 蔡永香

    2012-01-01

    对MapGIS K9 IMS的Flex开发框架展开研究,重点介绍了Flex客户端的结构及常用的控件,然后结合一个应用实例说明了MapGIS K9 IMS的Flex开发WebGIS的主要过程和关键问题,最后总结了基于MapGIS K9IMS的Flex开发模式设计与开发WebGIS的优势.%This paper introduces the Flex development framework of MapGIS K9 IMS, and mainly discusses the structure of client on Flex and the widgets used frequently. Then, by combining a specific application instance, the paper explains the main process and key problems when developing WebGIS based on this model. At last, the advantages of design and development of WebGIS based on this development mode are summarized

  9. Alignment of palatally impacted canine with open window technique and modified K-9 spring

    Directory of Open Access Journals (Sweden)

    Dipti Shastri

    2014-01-01

    Full Text Available The patient was an 18-year-old female who had an Angle Class I malocclusion with a left palatally impacted maxillary canine. The orthodontic treatment of a palatally impacted canine is aimed at bringing the tooth into its correct position in the dental arch without causing any periodontal damage. To achieve this goal, a variety of surgical and orthodontic techniques have been proposed in relation to the position of the impacted tooth and there are various treatment methods used for traction. The duration of the traction was 3 months and alignment duration was 12 months the total treatment time was 15 months. In the following case, we presented that maxillary palatally impacted canine was brought into the arch with open window method for canine exposure and modified K-9 spring for traction, that is simple spring for orthodontic traction of the palatally impacted canines.

  10. Mapgis k9地籍管理系统测试

    Institute of Scientific and Technical Information of China (English)

    颜循英

    2015-01-01

    由于第二次全国土地调查涉及面广,数据类型多,调查的结果缺乏标准和规范,给政府对全国土地管理带来极大不便,所以全国各省市的土地主管部门对地籍管理系统提出了新的要求。Mapgis k9地籍管理系统软件是按国家规定的制度开发,保证了地籍资料的连贯性、系统性、可靠性、精确性和完整性。

  11. KSHV ORF K9 (vIRF) is an oncogene which inhibits the interferon signaling pathway.

    Science.gov (United States)

    Gao, S J; Boshoff, C; Jayachandra, S; Weiss, R A; Chang, Y; Moore, P S

    1997-10-16

    Kaposi's sarcoma-associated herpesvirus (KSHV) is a gammaherpesvirus linked to the development of Kaposi's sarcoma and a rare B cell lymphoma, primary effusion lymphoma. The KSHV gene ORF K9 encodes vIRF which is a protein with low but significant homology to members of the interferon (IFN) regulatory factor (IRF) family responsible for regulating intracellular interferon signal transduction (Moore PS, Boshoff C, Weiss RA and Chang Y. (1996). Science, 274, 1739-1744). vIRF inhibits IFN-beta signal transduction as measured using an IFN-responsive ISG54 reporter construct co-transfected with ORF K9 into HeLa and 293 cells. vIRF also suppresses genes under IFN regulatory control as shown by inhibition of the IFN-beta inducibility of p21WAF1/CIP1, however, no direct DNA-binding or protein-protein interactions characteristic for IRF repressor proteins were identified. Stable transfectant NIH3T3 clones expressing vIRF grew in soft agar and at low serum concentrations, lost contact inhibition and formed tumors after injection into nude mice indicating that vIRF has the properties of a viral oncogene. Since vIRF is primarily expressed in KSHV-infected B cells, not KS spindle cells, this study suggests that vIRF is a transforming oncogene active in B cell neoplasias that may provide a unique immune escape mechanism for infected cells. This data is consistent with tumor suppressor pathways serving a dual function as host cell antiviral pathways.

  12. Study on ultra-precision machining of K9 glass by fixed abrasive lapping and polishing%固结磨料研磨抛光K9玻璃的超精密加工工艺研究

    Institute of Scientific and Technical Information of China (English)

    李标; 李军; 朱永伟; 夏磊; 李鹏鹏; 孙玉利; 左敦稳

    2012-01-01

    PHL-350 High-speed lapping & polishing system was employed in ultra-precision machining experiment of K9 glass by fixed abrasive lapping and polishing. Influence of fixed abrasive pad ( FAP ) with different particle size and different abrasive on material removal rate and surface quality of the K9 glass was studied. An ultra-precision machining process of K9 glass with high efficiency, low cost and high quality was obtained: first M20/30 diamond FAP was used for lapping, then 3 μm CeO2 FAP was used for polishing. A good surface of K9 glass with surface roughness RMS less than 0. 6 nm and very few micro scratches or damages was achieved.%采用PHL-350型平面高速研磨抛光系统,通过固结磨料研磨抛光方法进行了超精密加工K9玻璃试验研究.探讨了不同粒径和不同磨粒研磨抛光垫在加工中对K9玻璃材料去除率和表面质量的影响.获得了高效率、低成本、高质量的K9玻璃的超精密加工工艺:首先使用M20/30金刚石研磨垫研磨,然后使用3μm CeO2抛光垫抛光.加工后K9玻璃的表面粗糙度优于RMS 0.6 nm,微观损伤少.

  13. Analysis of gene selection in reassortant formation between canine rotavirus K9 and human rotaviruses with different antigenic specificities.

    Science.gov (United States)

    Kobayashi, N; Taniguchi, K; Urasawa, T; Urasawa, S

    1993-01-01

    A number of antigenic mosaic reassortants which have neutralization proteins VP4 and VP7 derived from different parental strains were analysed in order to study gene selection in reassortant formation between animal and human rotaviruses (HRV). These reassortants were isolated from mixed infection of MA-104 cells with canine rotavirus strain K9 (subgroup I and G serotype 3) and HRV strains (with subgroup I or II antigen and G serotype 1-4, 9 or 12 antigen), through repeated selections with anti-VP4 and anti-VP7 neutralizing monoclonal antibodies directed specifically at HRV and K9, respectively. By serological and genomic analyses, all the isolated clones were found to be antigenic mosaic reassortants possessing VP4 of K9 and VP7 of HRV. In the reassortants between strain K9 and one of the six strains of subgroup II HRV, a single or a few genotypes with particular constellations of RNA segments were predominant, with only a few RNA segments including gene 4 (encoding VP4) being derived from K9. In contrast, in the reassortants between strain K9 and any one of the subgroup I HRV, more than nine different genotypes were identified and various RNA segments, except for segments 8 and 10, were derived from K9. These findings indicated that the RNA segments of K9 might be reassorted more readily with those of subgroup I HRV than with those of subgroup II HRV, suggesting the possible existence of functional mechanisms which determine the extent of diversity of genome selection depending on the pairs of parent strains in the reassortant formation.

  14. Content

    DEFF Research Database (Denmark)

    Keiding, Tina Bering

    Aim, content and methods are fundamental categories of both theoretical and practical general didactics. A quick glance in recent pedagogical literature on higher education, however, reveals a strong preoccupation with methods, i.e. how teaching should be organized socially (Biggs & Tang, 2007......; Race, 2001; Ramsden, 2003). This trend appears closely related to the ‘from-teaching-to-learning’ movement, which has had a strong influence on pedagogy since the early nineties (Keiding, 2007; Terhart, 2003). Another interpretation of the current interest in methodology can be derived from...... for selection of content (Klafki, 1985, 2000; Myhre, 1961; Nielsen, 2006). These attempts all share one feature, which is that criteria for selection of content appear very general and often, more or less explicitly, deal with teaching at the first Bologna-cycle; i.e. schooling at the primary and lower...

  15. Effect of focus position of ns pulse laser on damage characteristics of K9 glass

    Science.gov (United States)

    Pan, Yunxiang; Zhang, Hongchao; Li, Mengmeng; Shen, Zhonghua; Lu, Jian; Ni, Xiaowu

    2015-05-01

    Laser-induced damage of optical glasses has been investigated for more than fifty years. Due to the residual scratches, inclusions and other forms of defects at surfaces of optical glasses after the processes of grinding and polishing, it is well known that the sample surface can be damaged more easily than bulk. In order to get the relationship between the damage threshold and the location of the laser spot, we carried out damage experiments on K9 glasses with a 7ns pulse laser. Since ns pulse laser-induced damage of optical glasses always accompanies with the generation of the plasma, a optical microscope connected with a CCD camera was used to observe the plasma flash, which can provide a real time detection of damage sites. The laser pulse was first focused into the bulk, then the spot was moved toward the direction of incident laser beam step by step until the beam was completely focused in ambient air. Damage threshold curves were measured for each focus position, and low thresholds and high thresholds were extracted from those curves. Finally, the relationship between damage thresholds and focus position was analyzed.

  16. Mutations in keratin K9 in kindreds with epidermolytic palmoplantar keratoderma and epidemiology in Northern Ireland.

    Science.gov (United States)

    Covello, S P; Irvine, A D; McKenna, K E; Munro, C S; Nevin, N C; Smith, F J; Uitto, J; McLean, W H

    1998-12-01

    Epidermolytic palmoplantar keratoderma (EPPK, MIM #144200) is an autosomal dominant disorder in which hyperkeratosis confined to the palms and soles is characterized histologically by cytolysis of suprabasal keratinocytes. Mutations in the keratin 9 gene (KRT9), a type 1 keratin expressed exclusively in the suprabasal keratinocytes of palmoplantar epidermis, have previously been demonstrated in this disorder. Here, we have studied four Northern Irish kindreds presenting with EPPK. By direct sequencing of polymerase chain reaction products, heterozygous missense mutations in exon 1 of KRT9 were detected in all the families. These included a novel mutation M156T; as well as M156V in two kindreds; and R162Q in the remaining family. All mutations were confirmed by reverse strand sequencing and restriction enzyme analysis. The point prevalence of EPPK in Northern Ireland was found to be 4.4 per 100,000. To date, all reported EPPK mutations occur in the helix initiation motif at the start of the central coiled-coil rod domain of K9.

  17. Purification and partial characterization of a tripeptidase from Pediococcus pentosaceus K9.2.

    Science.gov (United States)

    Simitsopoulou, M; Vafopoulou, A; Choli-Papadopoulou, T; Alichanidis, E

    1997-12-01

    A tripeptidase was purified from the cytoplasm of Pediococcus pentosaceus K9.2 by anion-exchange chromatography, gel filtration chromatography, and high-performance liquid chromatography. The molecular mass of the enzyme was estimated by gel filtration at 100,000 Da. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified peptidase showed one protein band of 45,000 Da. Optimal enzyme activity was obtained at pH 7.0 and at 50 degrees C. The peptidase hydrolyzed all tripeptides tested. Cleavage was not observed with dipeptides, oligopeptides, or amino acid-p-nitroanilide derivatives. Strong inhibition of activity was caused by EDTA, 1,10-phenanthroline, dithiothreitol, and beta-mercaptoethanol, whereas phenylmethylsulfonyl fluoride and sulfur-reactive reagents had no effect on peptidase activity. Mg2+, Mn2+, and Ca2+ stimulated the hydrolyzing activity of the enzyme. The 20 N-terminal amino acids of the tripeptidase from P. pentosaceus had 84% identity with those from the corresponding N-terminal region of the tripeptidase from Lactococcus lactis subsp. cremoris Wg2.

  18. Molecular Targets of Chromatin Repressive Mark H3K9me3 in Primate Progenitor Cells within Adult Neurogenic Niches

    Directory of Open Access Journals (Sweden)

    Michael R Foret

    2014-07-01

    Full Text Available Histone 3 Lysine 9 (H3K9 methylation is known to be associated with pericentric heterochromatin and important in genomic stability. In this study, we show that trimethylation at H3K9 (H3K9me3 is enriched in an adult neural stem cell niche- the subventricular zone (SVZ on the walls of the lateral ventricle in both rodent and non-human primate baboon brain. Previous studies have shown that there is significant correlation between baboon and human regarding genomic similarity and brain structure, suggesting that findings in baboon are relevant to human. To understand the function of H3K9me3 in this adult neurogenic niche, we performed genome-wide analyses using ChIP-Seq (chromatin immunoprecipitation and deep-sequencing and RNA-Seq for in vivo SVZ cells purified from baboon brain. Through integrated analyses of ChIP-Seq and RNA-Seq, we found that H3K9me3-enriched genes associated with cellular maintenance, post-transcriptional and translational modifications, signaling pathways, and DNA replication are expressed, while genes involved in axon/neuron, hepatic stellate cell, or immune-response activation are not expressed. As neurogenesis progresses in the adult SVZ, cell fate restriction is essential to direct proper lineage commitment. Our findings highlight that H3K9me3 repression in undifferentiated SVZ cells is engaged in the maintenance of cell type integrity, implicating a role for H3K9me3 as an epigenetic mechanism to control cell fate transition within this adult germinal niche.

  19. Research on Coal Quality and Coking Property of Russian Imported K9 Coking Coal%俄罗斯进口K9焦煤煤质特性与结焦特性研究

    Institute of Scientific and Technical Information of China (English)

    王文光; 李立凯; 李春那; 王军; 丁娜; 顾雪霞; 隋月诗

    2012-01-01

    通过质量检测、煤的岩相分析、40kg试验焦炉炼焦试验,对俄罗斯进口K9焦煤的煤质特性和结焦特性进行了研究,K9煤低灰、低硫,为单一煤层煤,煤质稳定,配煤炼焦有利于焦炭质量稳定.K9焦煤的结焦性能略好于我厂使用的国内中钢2号焦煤的结焦性能.

  20. 固结磨料研磨K9玻璃表面粗糙度模型%Model of Surface Roughness in Fixed Abrasive Lapping of K9 Glass

    Institute of Scientific and Technical Information of China (English)

    李军; 王慧敏; 王文泽; 黄建东; 朱永伟; 左敦稳

    2015-01-01

    Surface roughness model is an important basis for lapping process design and process parameter selection. K9 glass is one of the most widely applied optical materials. The model of surface roughness in lapping of K9 glass is established to improve processing efficiency and save production cost. Based on surface microstructure of the lapping pad, fixed abrasive lapping process is simplified, and the number of effective abrasives and the single abrasive depth of cutting into the workpiece are calculated. Combing the force equilibrium in lapping process, surface roughness model of fixed abrasive lapping of K9 glass is established. The model is verified by lapping of K9 glass with different abrasive particle sizes and concentrations, as well as different pressures. The results show that surface roughness of K9 glass is proportional to particle size and one-third power of lapping pressure, and inversely proportional to two-ninth power of the pad concentration. The changing trend of theoretical surface roughness values over abrasive particle sizes and pad concentrations as well as lapping pressures matches experimental trend. Surface roughness of K9 glass can be successfully predicted by the model in fixed abrasive lapping process, which can be used to guide the lapping process design and process parameters selection with high reliability.%表面粗糙度模型是研磨过程设计和工艺参数选择的重要依据,K9 玻璃是应用最广泛的光学材料之一.建立研磨 K9玻璃表面粗糙度模型有利于提高加工效率、节约生产成本.简化固结磨料研磨过程,基于研磨垫表面微结构,计算研磨过程中参与研磨的有效磨粒数和单颗磨粒切入工件深度,利用研磨过程中受力平衡,建立固结磨料研磨K9玻璃表面粗糙度模型.采用不同磨粒粒径和不同磨料浓度的固结磨料研磨垫以及不同压力研磨K9玻璃验证表面粗糙度模型.结果表明:固结磨料研磨K9玻璃

  1. Measurement for bulk and surface absorptances of K9 substrates with laser calorimeter%激光量热法测量K9基片的表面吸收和体吸收

    Institute of Scientific and Technical Information of China (English)

    刘浩; 潘峰; 陈松林; 王震; 马平; 欧阳升; 卫耀伟

    2014-01-01

    表面抛光可能给K9基片带来额外的杂质和吸收,分离K9基片的表面吸收率与体吸收率有助于改进基片的加工质量和抛光工艺,对抗损伤能力研究具有重要意义.分析了激光量热法测量弱吸收的原理,采用符合ISO 11551要求的激光量热计测量K9基片的弱吸收.对相同工艺抛光的不同厚度K9基片进行了弱吸收表征,实验发现K9基片的弱吸收随着厚度增加近似线性增大.推导了表面吸收率和体吸收率的计算式,实验得出本样品的表面吸收率为1.21×10-5,体吸收率远大于表面吸收率,体吸收系数为1.72×10-3/cm.实验结果显示所用K9样品的吸收主要来自于材料本身,改善抛光工艺对降低其吸收率作用不大.

  2. 脉冲CO2激光损伤K9玻璃的实验与仿真%Simulation and Experimental Research on Damage in K9 Glass due to Pulsed CO2 Laser Radiation

    Institute of Scientific and Technical Information of China (English)

    王玺; 李化; 聂劲松

    2008-01-01

    对脉冲CO2激光损伤K9玻璃进行了实验与仿真研究.采用输出脉宽为10μs的脉冲CO2激光器对K9玻璃样品进行激光损伤实验,并且建立了脉冲CO2激光损伤K9玻璃的理论模型,利用有限元法对K9玻璃样品中的温度和应力分布进行数值分析.研究表明,K9玻璃的损伤阈值为6.533 J/cm2;入射激光能量密度越高,样品的损伤程度就越大,并且多脉冲对样品的损伤程度明显大于单脉冲;在激光能量较强的情况下,K9玻璃表面在光斑区域内形成熔融损伤和由压缩应力造成的应力损伤,在光斑区域外围则形成由环向拉伸应力造成的应力损伤,仿真分析结果与实验结果吻合良好.

  3. Analysis of damage threshold of K9 glass irradiated by 248-nm KrF excimer laser

    Science.gov (United States)

    Wang, Xi; Shao, Jingzhen; Li, Hua; Nie, Jinsong; Fang, Xiaodong

    2016-02-01

    The theoretical model of K9 glass irradiated by a 248-nm KrF excimer laser was established, and a numerical simulation was performed to calculate temperature and thermal stress fields in the K9 glass sample using the finite element method. The laser-induced damage thresholds were defined and calculated, and the effect of repetition frequency and the number of pulses on the damage threshold were also studied. Furthermore, the experiment research was carried out to confirm the numerical simulation. The damage threshold and damage morphology were analyzed by means of a metallurgical microscope and scanning electron microscopy. The simulation and experimental results indicated that the damage mechanism of K9 glass irradiated by a KrF excimer laser was melting damage and stress damage, and the stress damage first appeared inside the K9 glass sample. The tensile stress damage threshold, the compressive stress damage threshold, and the melting damage threshold were 0.64, 0.76, and 1.05 J/cm2, respectively. The damage threshold decreased with increasing repetition frequency and number of laser pulses. The experimental results indicated that the damage threshold of K9 glass was 2.8 J/cm2.

  4. A jumonji (Jarid2) protein complex represses cyclin D1 expression by methylation of histone H3-K9.

    Science.gov (United States)

    Shirato, Haruki; Ogawa, Satoko; Nakajima, Kuniko; Inagawa, Masayo; Kojima, Mizuyo; Tachibana, Makoto; Shinkai, Yoichi; Takeuchi, Takashi

    2009-01-09

    Covalent modifications of histone tails have critical roles in regulating gene expression. Previously, we identified the jumonji (jmj, Jarid2) gene, the jmjC domain, and a Jmj family. Recently, many Jmj family proteins have been shown to be histone demethylases, and jmjC is the catalytic domain. However, Jmj does not have histone demethylase activity because the jmjC domain lacks conserved residues for binding to cofactors. Independently of these studies, we previously showed that Jmj binds to the cyclin D1 promoter and represses the transcription of cyclin D1. Here, we show the mechanisms by which Jmj represses the transcription of cyclin D1. We found that a protein complex of Jmj had histone methyltransferase activity toward histone H3 lysine 9 (H3-K9). We also found that Jmj bound to the H3-K9 methyltransferases G9a and GLP. Expression of Jmj recruited G9a and GLP to the cyclin D1 promoter and increased H3-K9 methylation. Inactivation of both G9a and GLP, but not of only G9a, inhibited the methylation of H3-K9 in the cyclin D1 promoter and repression of cyclin D1 expression by Jmj. These results suggest that Jmj methylates H3-K9 and represses cyclin D1 expression through G9a and GLP, and that Jmj family proteins can regulate gene expression by not only histone demethylation but also other histone modification.

  5. Analysis of temperature and thermal stress fields of K9 glass damaged by 1064nm nanosecond pulse laser

    Science.gov (United States)

    Pan, Yunxiang; Shen, Zhonghua; Lu, Jian; Ni, Xiaowu

    2013-02-01

    There are residual scratches, inclusions and other forms of defects at surfaces of optical materials after the processes of grinding and polishing, which could either enhance the local electric field or increase the absorption rate of the material. As a result, the laser-induced damage threshold at the surface of the material is reduced greatly. In order to study underlying mechanisms and process of short pulsed laser-induced damage to K9 glass, a spatial axisymmetric model where the K9 glass was irradiated by a laser whose wavelength and pulse width are respectively 1064nm and 10ns was established. Taking into account the fact that the surface of the K9 glass is more likely to be damaged, 2μm-thick layers whose absorption coefficients are larger than bulk were set at both the input and output surfaces in the model. In addition, the model assumed that once the calculated tensile/compressive stress was greater than the tensile/compressive strength of K9 glass, the local absorption coefficient increased. The finite element method(FEM) was applied to calculate the temperature and thermal stress fields in the K9 glass. Results show that only the temperature of a small part of interacted region exceeds the melting point, while most of the damage pit is generated by thermal stress. The simulated damage morphology and the size of the damage region are consistent with those reported in literatures, which indicates that the model built in our work is reasonable.

  6. Study on Traffic Thematic Maps Based on MapGIS K9 and Image Fusion%基于影像融合的MapGIS K9交通专题图制作研究

    Institute of Scientific and Technical Information of China (English)

    袁媛; 向怀坤; 张文绩

    2014-01-01

    研究将全色影像和多光谱影像在MapGIS K9遥感影像处理平台进行融合的方法,使融合后的影像既有正确的投影信息又有丰富的色彩和较高的空间分辨率.以融合后的影像为底图,研究利用MapGIS K9制作交通专题图的方法.

  7. Experiment on damage in K9 glass due to repetition rate pulsed CO2 laser radiation%重频脉冲CO2激光损伤K9玻璃的实验

    Institute of Scientific and Technical Information of China (English)

    王玺; 卞进田; 李华; 聂劲松; 孙晓泉; 尹学忠; 雷鹏

    2013-01-01

      对脉冲CO2激光在不同重频模式下损伤K9玻璃进行了实验研究。采用输出能量为10 J,脉宽为90 ns,重复频率在100 Hz至300 Hz之间连续可调的脉冲CO2激光器,对K9玻璃样品进行了激光损伤实验,观察到两次不同重频条件下样品的损伤形貌。实验结果表明,重频越高,对样品的损伤程度就越严重;应力损伤成为K9玻璃激光损伤的最主要的原因,在重频强激光的辐照下,K9玻璃表面出现强烈的等离子体闪光,伴随明显的熔融气化破坏,并形成等离子体爆轰波。爆轰波对玻璃材料产生了严重的力学冲击作用,这种应力作用足以对K9玻璃造成毁灭性破坏。运用有限元分析对激光辐照K9玻璃的温度与应力分布进行仿真,其计算结果与实验基本吻合。%  In this paper, the experiment on damage in K9 glass induced by pulsed CO2 laser under different repetition rates was carried out, which had a pulse width of 90 ns. The laser pulse energy was 10 J and the repetition rate was kept within the range of 100 Hz to 300 Hz. The damage morphologies of two kind repetition rates after laser irradiation were characterized. The experimental results indicate that the effect of laser irradiation on samples can be affected considerably by the change of laser repetition rate, and the intensity of damage morphology on the sample increases with the laser repetition rate, and the damage in K9 glass induced by pulsed CO2 laser is dominated by stress. As a result, the plasma detonation wave induced by laser occured, the material was broken result from the melting and evaporation of K9 glass. It is shown that the plasma detonation wave affected stress damage considerably, and this mechanical effect almost destroyed K9 glass sample. A numerical simulation was performed to calculate temperature and stress distributions in K9 glass sample irradiated by pulsed CO2 laser using finite element method. The model

  8. 优良K9苹果优质丰产配套栽培技术

    Institute of Scientific and Technical Information of China (English)

    李占印; 任宝君

    2014-01-01

    K9苹果,又名七月鲜,是辽宁省果树科学研究所以佚名大苹果为母本、铃铛果为父本杂交选育而成的.近年来在朝阳市的建平县、凌源市栽培表现果色艳丽、酸甜适口、果型圆正等诸多优良特性,根据K9在辽西北地区的生长特性,总结了苹果K9的园地选择、栽植、整形修剪、肥水管理、病虫害防治等系列关键技术.

  9. ICBP90, a novel methyl K9 H3 binding protein linking protein ubiquitination with heterochromatin formation.

    Science.gov (United States)

    Karagianni, Panagiota; Amazit, Larbi; Qin, Jun; Wong, Jiemin

    2008-01-01

    Methylation of histone H3 on lysine 9 is critical for diverse biological processes including transcriptional repression, heterochromatin formation, and X inactivation. The biological effects of histone methylation are thought to be mediated by effector proteins that recognize and bind to specific patterns of methylation. Using an unbiased in vitro biochemical approach, we have identified ICBP90, a transcription and cell cycle regulator, as a novel methyl K9 H3-specific binding protein. ICBP90 and its murine homologue Np95 are enriched in pericentric heterochromatin of interphase nuclei, and this localization is dependent on H3K9 methylation. Specific binding of ICBP90 to methyl K9 H3 depends on two functional domains, a PHD (plant homeodomain) finger that defines the binding specificity and an SRA (SET- and RING-associated) domain that promotes binding activity. Furthermore, we present evidence that ICBP90 is required for proper heterochromatin formation in mammalian cells.

  10. CONTENTS

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    The Development and Evolution of the Idea of the Mandate of Heaven in the Zhou Dynasty The changes in the idea of Mandate of Heaven during the Shang and Zhou dynasties are of great significance in the course of the development of traditional Chinese culture. The quickening and awakening of the humanistic spirit was not the entire content of the Zhou idea of Mandate of Heaven. In the process of annihilating the Shang dynasty and setting up their state, the Zhou propagated the idea of the Mandate of Heaven out of practical needs. Their idea of the Mandate of Heaven was not very different from that of the Shang. From the Western Zhou on, the Zhou idea of Mandate of Heaven by no means developed in a linear way along a rational track. The intermingling of rationality and irrationality and of awakening and non-awakening remained the overall state of the Zhou intellectual superstructure after their "spiritual awakening".

  11. 准分子激光辐照K9玻璃的热力效应分析%Thermal and mechanical damage in K9 glass irradiated by KrF excimer laser

    Institute of Scientific and Technical Information of China (English)

    王玺; 方晓东

    2016-01-01

    A numerical simulation is performed to calculate temperature and stress distributions in K9 glass sample irradia-ted by KrF excimer laser using finite element method,and the relationship between laser parameters and damage effect is also studied.The simulation results indicate that the damage of K9 glass irradiated by KrF excimer laser is thermal stress damage when the incident laser energy is low.The stress damage in laser spot area is dominated by compressive stress,and the damage near the edge of the spot or inside the K9 glass is dominated by tensile stress.The thermal stress as shock wave oscillates obvi-ously inside the K9 glass sample.It is shown that repetition frequency and the number of pulses affect damage considerably,and the accumulation of multi-shot laser induced damage in K9 glass is obvious.%基于传热学理论,利用有限元法对KrF准分子激光辐照K9 玻璃样品中的热力效应进行了数值分析,并比较了脉冲数目和重频对损伤效果的影响.研究表明,较低的准分子激光能量就能够使 K9 玻璃在表面和体内产生热应力损伤,热应力损伤在光斑区域内主要由压缩热应力控制,在光斑边缘和材料内部则主要由拉伸热应力控制.在激光脉冲结束时刻,产生的温度和热应力最大,且热应力以热冲击波的形式在材料内传播,随时间变化而来回振荡,逐渐减弱.这种热应力的反复冲击会对材料产生持续的损伤增长效应,增加了材料的损伤时间,并使材料更容易断裂.脉冲数目和重复频率对损伤效果有着较大影响,在高重复频率下,损伤累积效应明显.

  12. 固结磨料研磨掺铈玻璃与K9玻璃的对比研究%Research on fixed abrasive lapping of cerium doped glass and K9 glass

    Institute of Scientific and Technical Information of China (English)

    夏磊; 李军; 李鹏鹏; 朱永伟; 王文泽; 左敦稳

    2013-01-01

    在相同研磨条件下,采用固结磨料研磨垫研磨掺铈玻璃和K9玻璃,比较掺铈玻璃和K9玻璃的研磨材料去除率,研磨摩擦系数值和加工后表面质量.研究结果表明:金刚石固结磨料研磨垫对掺铈玻璃的材料去除率和研磨摩擦系数小于K9玻璃,表面质量相对较优.掺铈玻璃残屑中的Ce与Si在金刚石颗粒表面及磨粒之间形成包裹层和填覆层,改变材料去除机理,导致材料去除率和摩擦系数下降,获得更优的表面质量.%Fixed abrasive pad was employed to lap cerium doped glass and K9 glass in the same condition. Material removal rate(MRR) , lapping friction coefficient and surface quality were investigated. Results showed that MRR and lapping friction coefficient of cerium doped glass were less than those of K9 glass, but surface quality was better. The Ce and Si from the chips of cerium doped glass formed a covering layer on diamond particle surface and a filling layer in the gap between abrasives, thus changing the material removal mechanism. MRR and friction coefficient declined, and surface quality got better.

  13. Cloning, characterization and serological evaluation of K9 and K26: two related hydrophilic antigens of Leishmania chagasi.

    Science.gov (United States)

    Bhatia, A; Daifalla, N S; Jen, S; Badaro, R; Reed, S G; Skeiky, Y A

    1999-08-20

    We report here the molecular cloning and characterization of two related hydrophilic antigens of Leishmania chagasi. These two antigens have predicted molecular weights of approximately 9 and 26 kDa and detect antibodies in sera of patients with kala-azar (k). Thus, to maintain consistency with nomenclature of the previously described 39 kDa diagnostic antigen of L. chagasi (k39 [1]), these antigens are being referred to as k9 and k26. A significant difference between k9 and k26 is the presence of 11 copies of a 14 amino acid repeat in the open reading frame of k26. The region flanking the repeats of k26 shares a 69% identity with the open reading frame of k9. The recombinant proteins encoded by both antigens are very hydrophilic and show aberrant migration on SDS PAGE. Results of Southern blot analysis reveal that k9 and k26 are conserved to varying degrees among various Leishmania species. Interestingly, the repeat region of k26 is specific to L. chagasi and L. donovani while the flanking region is conserved among several other species. Transcript levels of k26 are significantly upregulated in the amastigote stage of the parasite. Our results show that recombinant K26 is specific in detecting antibodies in infection sera from visceral leishmaniasis (VL) patients. Thus rK26 may complement rK39 in a more accurate diagnosis of VL in the old and the new world.

  14. Transient absorption and luminescence spectra of K9 glass at sub-damage site by ultraviolet laser irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Z., E-mail: namezhangzhen@126.com [Science and Technology on Plasma Physics Laboratory, Research Center of Laser Fusion (China); Research Center of Laser Fusion, China Academy of Engineering Physics, Mianyang 621900 (China); Institute of Atomic and Molecular Physics, Sichuan University, Chengdu 610000 (China); Huang, J.; Geng, F.; Zhou, X.Y. [Research Center of Laser Fusion, China Academy of Engineering Physics, Mianyang 621900 (China); Feng, S.Q. [Institute of Atomic and Molecular Physics, Sichuan University, Chengdu 610000 (China); Cheng, X.L., E-mail: chengxl@scu.edu.cn [Institute of Atomic and Molecular Physics, Sichuan University, Chengdu 610000 (China); Jiang, X.D. [Research Center of Laser Fusion, China Academy of Engineering Physics, Mianyang 621900 (China); Wu, W.D. [Science and Technology on Plasma Physics Laboratory, Research Center of Laser Fusion (China); Research Center of Laser Fusion, China Academy of Engineering Physics, Mianyang 621900 (China); Institute of Atomic and Molecular Physics, Sichuan University, Chengdu 610000 (China); Zheng, W.G.; Tang, Y.J. [Research Center of Laser Fusion, China Academy of Engineering Physics, Mianyang 621900 (China)

    2014-01-01

    Highlights: • Transient absorption and luminescence spectra at sub-damage site of K9 glass by laser irradiation at 355 nm are presented. • As the energy density increases to 2.54 J/cm{sup 2}, the absorption intensity reaches to about 0.2. • The mechanism of two-photon ionization mainly plays a critical role at sub-damage site. • Intensity of Raman spectra is very high at low energy density and decreased with respect to high energy density. -- Abstract: Transient absorption and luminescence spectra at sub-damage site of K9 glass by laser irradiation at 355 nm are presented. The dependence of transient absorption on laser energy and number of pulses was investigated. As the energy density increases to 2.54 and 3.18 J/cm{sup 2}, the transient absorption intensity reaches to about 0.20 range from 400 to 480 nm. With the increase of number of pulses the process of residual absorption appears, which can be used to explain the fatigue effect of K9 glass. The defects in K9 glass were investigated by fluorescence and Raman spectra. The fluorescence band centered at about 410 nm is attributed to oxygen deficiency centers. The mechanism of two-photon ionization plays a critical role at sub-damage site. Compared to the Raman spectra of pristine site, intensity of Raman spectra is very high at a lower energy density, while it decreased at a higher energy density.

  15. A silencing pathway to induce H3-K9 and H4-K20 trimethylation at constitutive heterochromatin.

    Science.gov (United States)

    Schotta, Gunnar; Lachner, Monika; Sarma, Kavitha; Ebert, Anja; Sengupta, Roopsha; Reuter, Gunter; Reinberg, Danny; Jenuwein, Thomas

    2004-06-01

    Histone lysine methylation is a central modification to mark functionally distinct chromatin regions. In particular, H3-K9 trimethylation has emerged as a hallmark of pericentric heterochromatin in mammals. Here we show that H4-K20 trimethylation is also focally enriched at pericentric heterochromatin. Intriguingly, H3-K9 trimethylation by the Suv39h HMTases is required for the induction of H4-K20 trimethylation, although the H4 Lys 20 position is not an intrinsic substrate for these enzymes. By using a candidate approach, we identified Suv4-20h1 and Suv4-20h2 as two novel SET domain HMTases that localize to pericentric heterochromatin and specifically act as nucleosomal H4-K20 trimethylating enzymes. Interaction of the Suv4-20h enzymes with HP1 isoforms suggests a sequential mechanism to establish H3-K9 and H4-K20 trimethylation at pericentric heterochromatin. Heterochromatic H4-K20 trimethylation is evolutionarily conserved, and in Drosophila, the Suv4-20 homolog is a novel PEV modifier to regulate position-effect variegation. Together, our data indicate a function for H4-K20 trimethylation in gene silencing and further suggest H3-K9 and H4-K20 trimethylation as important components of a repressive pathway that can index pericentric heterochromatin.

  16. K4, K9 and K18 in human histone H3 are targets for biotinylation by biotinidase.

    Science.gov (United States)

    Kobza, Keyna; Camporeale, Gabriela; Rueckert, Brian; Kueh, Alice; Griffin, Jacob B; Sarath, Gautam; Zempleni, Janos

    2005-08-01

    Histones are modified post-translationally, e.g. by methylation of lysine and arginine residues, and by phosphorylation of serine residues. These modifications regulate processes such as gene expression, DNA repair, and mitosis and meiosis. Recently, evidence has been provided that histones are also modified by covalent binding of the vitamin biotin. The aims of this study were to identify biotinylation sites in histone H3, and to investigate the crosstalk among histone biotinylation, methylation and phosphorylation. Synthetic peptides based on the sequence of human histone H3 were used as substrates for enzymatic biotinylation by biotinidase; biotin in peptides was probed using streptavidin peroxidase. These studies provided evidence that K4, K9 and K18 in histone H3 are good targets for biotinylation; K14 and K23 are relatively poor targets. Antibodies were generated to histone H3, biotinylated either at K4, K9 or K18. These antibodies localized to nuclei in human placental cells in immunocytochemistry and immunoblotting experiments, suggesting that lysines in histone H3 are biotinylated in vivo. Dimethylation of R2, R8 and R17 increased biotinylation of K4, K9 and K18, respectively, by biotinidase; phosphorylation of S10 abolished biotinylation of K9. These observations are consistent with crosstalk between biotinylation of histones and other known modifications of histones. We speculate that this crosstalk provides a link to known roles for biotin in gene expression and cell proliferation.

  17. H3K9me-independent gene silencing in fission yeast heterochromatin by Clr5 and histone deacetylases

    DEFF Research Database (Denmark)

    Hansen, Klavs R; Hazan, Idit; Shanker, Sreenath;

    2011-01-01

    Nucleosomes in heterochromatic regions bear histone modifications that distinguish them from euchromatic nucleosomes. Among those, histone H3 lysine 9 methylation (H3K9me) and hypoacetylation have been evolutionarily conserved and are found in both multicellular eukaryotes and single-cell model...

  18. Contents

    Directory of Open Access Journals (Sweden)

    Editor IJRED

    2012-11-01

    Full Text Available International Journal of Renewable Energy Development www.ijred.com Volume 1             Number 3            October 2012                ISSN 2252- 4940   CONTENTS OF ARTICLES page Design and Economic Analysis of a Photovoltaic System: A Case Study 65-73 C.O.C. Oko , E.O. Diemuodeke, N.F. Omunakwe, and E. Nnamdi     Development of Formaldehyde Adsorption using Modified Activated Carbon – A Review 75-80 W.D.P Rengga , M. Sudibandriyo and M. Nasikin     Process Optimization for Ethyl Ester Production in Fixed Bed Reactor Using Calcium Oxide Impregnated Palm Shell Activated Carbon (CaO/PSAC 81-86 A. Buasri , B. Ksapabutr, M. Panapoy and N. Chaiyut     Wind Resource Assessment in Abadan Airport in Iran 87-97 Mojtaba Nedaei       The Energy Processing by Power Electronics and its Impact on Power Quality 99-105 J. E. Rocha and B. W. D. C. Sanchez       First Aspect of Conventional Power System Assessment for High Wind Power Plants Penetration 107-113 A. Merzic , M. Music, and M. Rascic   Experimental Study on the Production of Karanja Oil Methyl Ester and Its Effect on Diesel Engine 115-122 N. Shrivastava,  , S.N. Varma and M. Pandey  

  19. H3K9 Trimethylation Silences Fas Expression To Confer Colon Carcinoma Immune Escape and 5-Fluorouracil Chemoresistance.

    Science.gov (United States)

    Paschall, Amy V; Yang, Dafeng; Lu, Chunwan; Choi, Jeong-Hyeon; Li, Xia; Liu, Feiyan; Figueroa, Mario; Oberlies, Nicholas H; Pearce, Cedric; Bollag, Wendy B; Nayak-Kapoor, Asha; Liu, Kebin

    2015-08-15

    The Fas-FasL effector mechanism plays a key role in cancer immune surveillance by host T cells, but metastatic human colon carcinoma often uses silencing Fas expression as a mechanism of immune evasion. The molecular mechanism under FAS transcriptional silencing in human colon carcinoma is unknown. We performed genome-wide chromatin immunoprecipitation sequencing analysis and identified that the FAS promoter is enriched with H3K9me3 in metastatic human colon carcinoma cells. The H3K9me3 level in the FAS promoter region is significantly higher in metastatic than in primary cancer cells, and it is inversely correlated with Fas expression level. We discovered that verticillin A is a selective inhibitor of histone methyltransferases SUV39H1, SUV39H2, and G9a/GLP that exhibit redundant functions in H3K9 trimethylation and FAS transcriptional silencing. Genome-wide gene expression analysis identified FAS as one of the verticillin A target genes. Verticillin A treatment decreased H3K9me3 levels in the FAS promoter and restored Fas expression. Furthermore, verticillin A exhibited greater efficacy than decitabine and vorinostat in overcoming colon carcinoma resistance to FasL-induced apoptosis. Verticillin A also increased DR5 expression and overcame colon carcinoma resistance to DR5 agonist drozitumab-induced apoptosis. Interestingly, verticillin A overcame metastatic colon carcinoma resistance to 5-fluorouracil in vitro and in vivo. Using an orthotopic colon cancer mouse model, we demonstrated that tumor-infiltrating cytotoxic T lymphocytes are FasL(+) and that FasL-mediated cancer immune surveillance is essential for colon carcinoma growth control in vivo. Our findings determine that H3K9me3 of the FAS promoter is a dominant mechanism underlying FAS silencing and resultant colon carcinoma immune evasion and progression.

  20. Introduktion til STS

    DEFF Research Database (Denmark)

    Introduktion til STS er den første danske grundbog om dette nye felt. Bogen giver en pædagogisk introduktion til STS-studier og den forskelligartede og ofte vanskelige primærlitteratur, der kendetegner feltet. Forfatterne præsenterer blandt andet socialkonstruktivistiske tilgange til videnskab og...

  1. 青兰高速二灰土(水泥/石灰/土)试验段(k9+412-k9+740)施工方法

    Institute of Scientific and Technical Information of China (English)

    刘庆滨

    2008-01-01

    路基综合稳定土简称二灰土,它是水泥、石灰、土的混合料。现广泛应用在高速、一级路的路基底基层的施工上,因他的施工工艺技术含量要求较高,所以在正式大面积施工前,一定要做好施工试验段的全部技术指标的确定工作。以下就是青兰高速的试验段(K9+412-k9+740)的施工过程。

  2. K9玻璃磁性研磨抛光表面粗糙度试验研究%Research on surface roughness of K9 glass by magnetic abrasive finishing

    Institute of Scientific and Technical Information of China (English)

    丘永亮; 邱腾雄; 阎秋生

    2015-01-01

    针对 K9光学玻璃研磨抛光过程中存在的问题,将磁性研磨加工方法应用在光学玻璃的研磨抛光上。试验表明,K9玻璃的表面粗糙度值Ra由原来的90 nm 左右下降到40 nm 左右。为进一步研究磁性研磨试验的4个因素,设计了正交试验,最终得出各个因素对于工件表面粗糙度影响的主次顺序,并确定其最优组合为:磁极转速2300 r/min,加工间隙1.5 mm,磁感应强度0.4 T,进给速度200 mm/min。%To study the existing problems of polishing K9 optical glass,magnetic abrasive finishing was put forward in polishing optical glass•Results showed that surface roughness R a of K9 glass decreased from 90 nm to 40 nm•Based on the feasibility study,the magnetic abrasive finishing experiments were performed by orthogonal experiment with 4 parameters•Then various factors of magnetic abrasive finishing were listed in sequence•The optimum combination was that:pole speed of 2300 r∕min,working clearance of 1•5 mm,magnetic flux density of 0•4 T and feed rate of 200 mm∕min.

  3. H3K9me3 Inhibition Improves Memory, Promotes Spine Formation, and Increases BDNF Levels in the Aged Hippocampus.

    Science.gov (United States)

    Snigdha, Shikha; Prieto, G Aleph; Petrosyan, Arpine; Loertscher, Brad M; Dieskau, André P; Overman, Larry E; Cotman, Carl W

    2016-03-23

    An increasing number of studies show that an altered epigenetic landscape may cause impairments in regulation of learning and memory-related genes within the aged hippocampus, eventually resulting in cognitive deficits in the aged brain. One such epigenetic repressive mark is trimethylation of H3K9 (H3K9me3), which is typically implicated in gene silencing. Here, we identify, for the first time, an essential role for H3K9me3 and its histone methyl transferase (SUV39H1) in mediating hippocampal memory functions. Pharmacological inhibition of SUV39H1 using a novel and selective inhibitor decreased levels of H3K9me3 in the hippocampus of aged mice, and improved performance in the objection location memory and fear conditioning tasks and in a complex spatial environment learning task. The inhibition of SUV39H1 induced an increase in spine density of thin and stubby but not mushroom spines in the hippocampus of aged animals and increased surface GluR1 levels in hippocampal synaptosomes, a key index of spine plasticity. Furthermore, there were changes at BDNF exon I gene promoter, in concert with overall BDNF levels in the hippocampus of drug-treated animals compared with control animals. Together, these data demonstrate that SUV39H1 inhibition and the concomitant H3K9me3 downregulation mediate gene transcription in the hippocampus and reverse age-dependent deficits in hippocampal memory. Cognitive decline is a debilitating condition associated with not only neurodegenerative diseases but also aging in general. However, effective treatments have been slow to emerge so far. In this study, we demonstrate that epigenetic regulation of key synaptic proteins may be an underlying, yet reversible, cause of this decline. Our findings suggest that histone 3 trimethylation is a probable target for pharmacological intervention that can counteract cognitive decline in the aging brain. Finally, we provide support to the hypothesis that, by manipulating the enzyme that regulates H3K9

  4. Comparative investigation of damage performance on K9 and SiO2 under 1064-nm nanosecond laser irradiation

    Science.gov (United States)

    Liu, Hongjie; Wang, Fengrui; Zhang, Zhen; Huang, Jin; Zhou, Xinda; Jiang, Xiaodong; Wu, Weidong; Zheng, Wanguo

    2012-01-01

    Laser damage performance of K9 glass and fused silica glass were tested respectively at same experimental condition with 1064 nm nanosecond laser. The initial damage threshold (IDT), the damage growth threshold (DGT) and the damage growth laws of the two optics glass were investigated comparatively. The results show that the damage growth behavior of the two glasses are quite different, for example, the lower damage growth threshold and the higher damage growth coefficient for K9 glass, which can attribute to the difference of the material's damage morphology, optical absorption, residual stress near damage site between the two optics glass. The research is very important to choose transparent optical material applied in high power laser.

  5. H3K9me-independent gene silencing in fission yeast heterochromatin by Clr5 and histone deacetylases.

    Science.gov (United States)

    Hansen, Klavs R; Hazan, Idit; Shanker, Sreenath; Watt, Stephen; Verhein-Hansen, Janne; Bähler, Jürg; Martienssen, Robert A; Partridge, Janet F; Cohen, Amikam; Thon, Geneviève

    2011-01-06

    Nucleosomes in heterochromatic regions bear histone modifications that distinguish them from euchromatic nucleosomes. Among those, histone H3 lysine 9 methylation (H3K9me) and hypoacetylation have been evolutionarily conserved and are found in both multicellular eukaryotes and single-cell model organisms such as fission yeast. In spite of numerous studies, the relative contributions of the various heterochromatic histone marks to the properties of heterochromatin remain largely undefined. Here, we report that silencing of the fission yeast mating-type cassettes, which are located in a well-characterized heterochromatic region, is hardly affected in cells lacking the H3K9 methyltransferase Clr4. We document the existence of a pathway parallel to H3K9me ensuring gene repression in the absence of Clr4 and identify a silencing factor central to this pathway, Clr5. We find that Clr5 controls gene expression at multiple chromosomal locations in addition to affecting the mating-type region. The histone deacetylase Clr6 acts in the same pathway as Clr5, at least for its effects in the mating-type region, and on a subset of other targets, notably a region recently found to be prone to neo-centromere formation. The genomic targets of Clr5 also include Ste11, a master regulator of sexual differentiation. Hence Clr5, like the multi-functional Atf1 transcription factor which also modulates chromatin structure in the mating-type region, controls sexual differentiation and genome integrity at several levels. Globally, our results point to histone deacetylases as prominent repressors of gene expression in fission yeast heterochromatin. These deacetylases can act in concert with, or independently of, the widely studied H3K9me mark to influence gene silencing at heterochromatic loci.

  6. H3K9me-independent gene silencing in fission yeast heterochromatin by Clr5 and histone deacetylases.

    Directory of Open Access Journals (Sweden)

    Klavs R Hansen

    Full Text Available Nucleosomes in heterochromatic regions bear histone modifications that distinguish them from euchromatic nucleosomes. Among those, histone H3 lysine 9 methylation (H3K9me and hypoacetylation have been evolutionarily conserved and are found in both multicellular eukaryotes and single-cell model organisms such as fission yeast. In spite of numerous studies, the relative contributions of the various heterochromatic histone marks to the properties of heterochromatin remain largely undefined. Here, we report that silencing of the fission yeast mating-type cassettes, which are located in a well-characterized heterochromatic region, is hardly affected in cells lacking the H3K9 methyltransferase Clr4. We document the existence of a pathway parallel to H3K9me ensuring gene repression in the absence of Clr4 and identify a silencing factor central to this pathway, Clr5. We find that Clr5 controls gene expression at multiple chromosomal locations in addition to affecting the mating-type region. The histone deacetylase Clr6 acts in the same pathway as Clr5, at least for its effects in the mating-type region, and on a subset of other targets, notably a region recently found to be prone to neo-centromere formation. The genomic targets of Clr5 also include Ste11, a master regulator of sexual differentiation. Hence Clr5, like the multi-functional Atf1 transcription factor which also modulates chromatin structure in the mating-type region, controls sexual differentiation and genome integrity at several levels. Globally, our results point to histone deacetylases as prominent repressors of gene expression in fission yeast heterochromatin. These deacetylases can act in concert with, or independently of, the widely studied H3K9me mark to influence gene silencing at heterochromatic loci.

  7. The effect of laser pulse width on laser-induced damage at K9 and UBK7 components surface

    Science.gov (United States)

    Zhou, Xinda; Ba, Rongsheng; Zheng, Yinbo; Yuan, Jing; Li, Wenhong; Chen, Bo

    2015-07-01

    In this paper, we investigated the effects of laser pulse width on laser-induced damage. We measured the damage threshold of K9 glass and UBK7 glass optical components at different pulse width, then analysis pulse-width dependence of damage threshold. It is shown that damage threshold at different pulse width conforms to thermal restriction mechanism, Because of cm size laser beam, defect on the optical component surface leads to laser-induced threshold decreased.

  8. Map Gis-K9 在油田发展建设中的应用

    Institute of Scientific and Technical Information of China (English)

    李金仁

    2016-01-01

    在信息技术时代,油田发展建设过程中也必须提升其决策和业务的信息技术水平。Map GIS-K9 不仅技术成熟,而且二次开发等售后服务能够基于用户实际情况做出科学调整,因此,它在油田发展建设中得到广发应用,也受到用户的广泛认可。从石油地质图编绘系统、油气管道管理系统等方面讨论了Map Gis-K9 在油田发展建设中的应用,希望对促进Map Gis-K9 在油田工程领域中更好地发挥价值,从而促进中国石油领域的进步有积极意义。

  9. Fluorescence and Raman spectra on surface of K9 glass by high fluence ultraviolet laser irradiation at 355 nm

    Science.gov (United States)

    Zhang, Zhen; Huang, Jin; Geng, Feng; Zhou, Xiaoyan; Feng, Shiquan; Ren, Dahua; Cheng, Xinlu; Jiang, Xiaodong; Wu, Weidong; Zheng, Wanguo; Tang, Yongjian

    2013-11-01

    In order to explore the damage mechanisms of K9 glass irradiated by high energy density ultraviolet laser, laser-induced fluorescence and Raman spectra were investigated. Compared the fluorescence spectra of damaged area, undamaged area and sub-damaged area, it can be conclude that the fluorescence spectrum of sub-damaged area is different from the structure of the other two areas. Especially, the main peak of the spectra at 415 nm reveals the unique characteristics of K9 glass. The structure at the sub-damaged area enhances intensity of the Raman scattering spectra. Three peaks of the spectra at about 500 nm and two characteristic peaks at about 550 nm exhibit the characterization of damaged area. A peak of the Raman scattering spectra at 350 nm which related to water can be observed. The relationship between intensity of Raman scattering and laser intensity at 355 nm is investigated by confocal Raman microscopy. At sub-damage area, signal of Raman scattering is rather high and decreased dramatically with respect to energy density. The major band at about 1470 cm-1 sharpened and moved to higher frequency with densification. These phenomena demonstrate that the structure of sub-damaged area has some characterization compared with the damaged area. The investigation of defect induced fluorescence and Raman spectra on surface of K9 glass is important to explore the damage mechanisms of optical materials irradiated by ultraviolet laser irradiation at 355 nm.

  10. Monoallele deletion of CBP leads to pericentromeric heterochromatin condensation through ESET expression and histone H3 (K9) methylation.

    Science.gov (United States)

    Lee, Junghee; Hagerty, Sean; Cormier, Kerry A; Kim, Jinho; Kung, Andrew L; Ferrante, Robert J; Ryu, Hoon

    2008-06-15

    Chromatin remodeling is tightly controlled under physiological conditions. Alterations in chromatin structure are involved in the pathogenesis of neuronal systems. We found that the monoallelic deletion of CREB binding protein (CBP) results in the induction of ERG-associated protein with SET domain (ESET) and increases trimethylation of histone H3 (K9) and condensation of pericentromeric heterochromatin structure in neurons. Nested deletion and mutational analysis of the ESET promoter further demonstrated that the Ets-2 transcription factor regulates transcriptional activity of the ESET gene. In CBP+/- mice, Ets-2 occupancy in the ESET promoter DNA was markedly elevated. Our results suggest that CBP is a transcriptional repressor of ESET gene expression by limiting Ets-2 transcriptional activity, while CBP siRNA enhances basal and Ets-2-dependent ESET transcriptional activity. Altered expression of the ESET gene and hypertrimethylation of H3 (K9) correlate with striatal neuron atrophy and dysfunction in CBP+/- mice. These results establish an alternative pathway that loss of CBP leads to the pericentric heterochromatin condensation through ESET expression and trimethylation of H3 (K9).

  11. KEY COMPARISON Final report on APMP international comparison APMP.EM-K9: High voltage AC-DC transfer standards

    Science.gov (United States)

    Wei, Yih-Cheng; Yeh, Hsin-Da

    2010-01-01

    The international key comparison APMP.EM-K9 of AC-DC high voltage transfer standards with 12 participants was carried out from June 2000 to January 2004. This comparison offers the same range and frequencies as BIPM key comparison CCEM-K9, voltages at 500 V and 1000 V, frequency from 1 kHz to 100 kHz. This comparison provides the national metrology institutes (NMIs) of the APMP member economies with an opportunity to link the values of their standards for AC-DC transfer difference to the international reference values. The results of the majority of the participating NMIs show an agreement with the reference value within the associated expanded uncertainty given by the individual NMI. The agreement of the results and the tables of the degree of equivalence of the participants are included. The results have been linked to the key comparison CCEM-K9. Main text. To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCEM, according to the provisions of the CIPM Mutual Recognition Arrangement (MRA).

  12. Differential dynamics of histone H3 methylation at positions K4 and K9 in the mouse zygote

    Directory of Open Access Journals (Sweden)

    Walter Jörn

    2004-09-01

    Full Text Available Abstract Background In the mouse zygote the paternal genome undergoes dramatic structural and epigenetic changes. Chromosomes are decondensed, protamines replaced by histones and DNA is rapidly and actively demethylated. The epigenetic asymmetry between parental genomes remains at least until the 2-cell stage suggesting functional differences between paternal and maternal genomes during early cleavage stages. Results Here we analyzed the timing of histone deposition on the paternal pronucleus and the dynamics of histone H3 methylation (H3/K4mono-, H3/K4tri- and H3/K9di-methylation immediately after fertilization. Whereas maternal chromatin maintains all types of histone H3 methylation throughout the zygotic development, paternal chromosomes acquire new and unmodified histones shortly after fertilization. In the following hours we observe a gradual increase in H3/K4mono-methylation whereas H3/K4tri-methylation is not present before latest pronuclear stages. Histone H3/K9di-methylation is completely absent from the paternal pronucleus, including metaphase chromosomes of the first mitotic stage. Conclusion Parallel to the epigenetic asymmetry in DNA methylation, chromatin modifications are also different between both parental genomes in the very first hours post fertilization. Whereas methylation at H3/K4 gradually becomes similar between both genomes, H3/K9 methylation remains asymmetric.

  13. Shock induced damage and damage threshold of optical K9 glass investigated by laser-driven shock wave

    Science.gov (United States)

    Song, Yunfei; Yu, Guoyang; Jiang, Lilin; Zheng, Xianxu; Liu, Yuqiang; Yang, Yanqiang

    2011-04-01

    The shock wave driven by short laser pulse is used to study the damage of brittle material K9 glass. The damage morphology of K9 glass surface indicates that the material has experienced different loading modes, respectively, at the central area and the surrounding area of the shock wave. At the central area of shock wave, the wavefront is plane and has a uniform pressure distribution, the material mainly suffers a longitudinal shock pressure; but on the edge the shock wave, the wavefront is approximately spherical, besides longitudinal pressure, transverse tensile stress will emerge inside the material. In the latter case, the damage threshold of the material is much smaller than that in the case of compressing by longitudinal pressure only. According to the relationship between damage area and shock pressure, an experimental method is proposed to measure the damage threshold of materials under shock loading. The damage threshold of K9 glass under spherical shock wave is measured to be about 1.12 GPa; and the damage threshold under plane shock wave is estimated to be between 1.82 and 1.98 GPa. They are much bigger than the damage threshold under static pressure. This method could also be used to measure the damage threshold of other materials when loaded by dynamic pressure.

  14. Transient absorption and luminescence spectra of K9 glass at sub-damage site by ultraviolet laser irradiation

    Science.gov (United States)

    Zhang, Z.; Huang, J.; Geng, F.; Zhou, X. Y.; Feng, S. Q.; Cheng, X. L.; Jiang, X. D.; Wu, W. D.; Zheng, W. G.; Tang, Y. J.

    2014-01-01

    Transient absorption and luminescence spectra at sub-damage site of K9 glass by laser irradiation at 355 nm are presented. The dependence of transient absorption on laser energy and number of pulses was investigated. As the energy density increases to 2.54 and 3.18 J/cm2, the transient absorption intensity reaches to about 0.20 range from 400 to 480 nm. With the increase of number of pulses the process of residual absorption appears, which can be used to explain the fatigue effect of K9 glass. The defects in K9 glass were investigated by fluorescence and Raman spectra. The fluorescence band centered at about 410 nm is attributed to oxygen deficiency centers. The mechanism of two-photon ionization plays a critical role at sub-damage site. Compared to the Raman spectra of pristine site, intensity of Raman spectra is very high at a lower energy density, while it decreased at a higher energy density.

  15. Involvement of histone methyltransferase GLP in HIV-1 latency through catalysis of H3K9 dimethylation

    Energy Technology Data Exchange (ETDEWEB)

    Ding, Donglin; Qu, Xiying; Li, Lin; Zhou, Xin; Liu, Sijie; Lin, Shiguan; Wang, Pengfei; Liu, Shaohui; Kong, Chuijin; Wang, Xiaohui; Liu, Lin; Zhu, Huanzhang, E-mail: hzzhu@fudan.edu.cn

    2013-06-05

    Understanding the mechanism of HIV-1 latency is crucial to eradication of the viral reservoir in HIV-1-infected individuals. However, the role of histone methyltransferase (HMT) G9a-like protein (GLP) in HIV-1 latency is still unclear. In the present work, we established four clonal cell lines containing HIV-1 vector. We found that the integration sites of most clonal cell lines favored active gene regions. However, we also observed hypomethylation of CpG of HIV 5′LTR in all four clonal cell lines. Additionally, 5′-deoxy-5′-methylthioadenosine (MTA), a broad-spectrum histone methyltransferase inhibitor, was used to examine the role of histone methylation in HIV-1 latency. MTA was found to decrease the level of H3K9 dimethylation, causing reactivation of latent HIV-1 in C11 cells. GLP knockdown by small interfering RNA clearly induced HIV-1 LTR expression. Results suggest that GLP may play a significant role in the maintenance of HIV-1 latency by catalyzing dimethylation of H3K9. - Highlights: ► We have established an in vitro model of HIV-1 latency. ► The integration sites of most clonal cell lines favor in active gene regions. ► Hypomethylation occurs in CpG islands of HIV 5′LTR in all four clonal cell lines. ► MTA can reactivate latent HIV-1 by decreasing the level of H3K9 me2 in C11 cells. ► HMT GLP may play a significant role in the maintenance of HIV-1 latency.

  16. Pre-analytical variables of circulating cell-free nucleosomes containing 5-methylcytosine DNA or histone modification H3K9Me3

    DEFF Research Database (Denmark)

    Rasmussen, Louise; Herzog, Marielle; Rømer, Eva

    2016-01-01

    Aim: To evaluate pre-analytical variables of circulating cell-free nucleosomes containing 5-methylcytosine DNA (5mC) or histone modification H3K9Me3 (H3K9Me3). Materials and methods: Six studies were designed to assess the possible influence of pre-analytical variables. Study 1: influence of stasis...

  17. Gradual development of a genome-wide H3-K9 trimethylation pattern in paternally derived pig pronucleus.

    Science.gov (United States)

    Jeong, Young Sun; Yeo, Seungeun; Park, Jung Sun; Lee, Kyung-Kwang; Kang, Yong-Kook

    2007-06-01

    The cytoplasm of a mature oocyte contains many protein complexes that are programmed to restructure incoming sperm chromatins on fertilization. Of the complicated biochemical events that these functional machineries control, the most impressive and important is epigenetic reprogramming. Despite its importance in epigenetic resetting, or "de-differentiation," of gamete genomes back to an incipient status, the mechanisms of epigenetic reprogramming do not seem to be conserved among mammals. Here, we report that, unlike in the mouse, the pig sperm-derived pronucleus is markedly trimethylated at lysine 9 of histone H3 (H3-m(3)K9), which might be associated with preservation of paternally derived cytosine methylation in pig zygotes. The male H3-m(3)K9 pattern is gradually established during pronucleus development, and this process occurs independently of DNA replication. Considering these unique epigenetic features, the pig zygote is, we believe, suited to serve as another model of epigenetic reprogramming that is antithetical to the well-characterized mouse model.

  18. K9BB METAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — METAR is a routine scheduled observation and is the primary observation code used in the United States to satisfy requirements for reporting surface meteorological...

  19. K9MN METAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — METAR is a routine scheduled observation and is the primary observation code used in the United States to satisfy requirements for reporting surface meteorological...

  20. STS-121 Insignia

    Science.gov (United States)

    2005-01-01

    The STS-121 patch depicts the Space Shuttle docked with the International Space Station (ISS) in the foreground, overlaying the astronaut symbol with three gold columns and a gold star. The ISS is shown in the configuration that it was during the STS-121 mission. The background shows the nighttime Earth with a dawn breaking over the horizon. STS-121, ISS mission ULF1.1, was the final Shuttle Return to Flight test mission. This utilization and logistics flight delivered a multipurpose logistics module (MPLM) to the ISS with several thousand pounds of new supplies and experiments. In addition, some new orbital replacement units (ORUs) were delivered and stowed externally on the ISS on a special pallet. These ORUs are spares for critical machinery located on the outside of the ISS. During this mission the crew also carried out testing of Shuttle inspection and repair hardware, as well as evaluated operational techniques and concepts for conducting on-orbit inspection and repair.

  1. A role for repressive complexes and H3K9 di-methylation in PRDM5-associated brittle cornea syndrome

    DEFF Research Database (Denmark)

    Porter, Louise F; Galli, Giorgio G; Williamson, Sally

    2015-01-01

    skin fibroblast expression microarrays from patients with PRDM5 mutations p.Arg590* and Δ exons 9-14, as well as from a PRDM5 ChIP-sequencing experiment. Gene ontology analysis of dysregulated PRDM5 target genes reveals enrichment for extracellular matrix (ECM) genes supporting vascular integrity...... and development. Q-PCR, and ChIP-qPCR confirm upregulation of critical mediators of ECM stability in vascular structures (COL13A1, COL15A1, NTN1, CDH5) in patient fibroblasts. We identify H3K9 di-methylation at these PRDM5 target genes in fibroblasts, and demonstrate that the BCS2 mutation p.Arg83Cys diminishes...

  2. H3K9me-independent gene silencing in fission yeast heterochromatin by Clr5 and histone deacetylases

    DEFF Research Database (Denmark)

    Hansen, Klavs R; Hazan, Idit; Shanker, Sreenath

    2011-01-01

    Nucleosomes in heterochromatic regions bear histone modifications that distinguish them from euchromatic nucleosomes. Among those, histone H3 lysine 9 methylation (H3K9me) and hypoacetylation have been evolutionarily conserved and are found in both multicellular eukaryotes and single-cell model...... organisms such as fission yeast. In spite of numerous studies, the relative contributions of the various heterochromatic histone marks to the properties of heterochromatin remain largely undefined. Here, we report that silencing of the fission yeast mating-type cassettes, which are located in a well...... at multiple chromosomal locations in addition to affecting the mating-type region. The histone deacetylase Clr6 acts in the same pathway as Clr5, at least for its effects in the mating-type region, and on a subset of other targets, notably a region recently found to be prone to neo-centromere formation...

  3. Polarization-dependent characteristics of a racetrack waveguide resonator fabricated by ion exchange in K9 glass

    Institute of Scientific and Technical Information of China (English)

    Xiuyou Han; Feng Liu; Fufei Pang; Fenghong Chu; Haiwen Cai; Ronghui Qu; Zujie Fang

    2006-01-01

    @@ A racetrack waveguide resonator filter was fabricated by ion exchange technology in K9 optical glass.The filter responses of the waveguide resonator were measured with two polarized input lightwaves. The polarization-dependent characteristics of the waveguide resonator were analyzed, and the results of effective indices of Nte = 1.5721 and Ntm = 1.5548, coupling ratios of Rte = 0.731 and Rtm = 0.761, and losses of Αte = 4.35 Db/cm and Αtm = 6.05 Db/cm were obtained for transverse electric (TE) and transverse magnetic (TM) modes, respectively. The causes of large loss and effective index differences between TE and TM modes were discussed.

  4. Progressive Chromatin Condensation and H3K9 Methylation Regulate the Differentiation of Embryonic and Hematopoietic Stem Cells

    Science.gov (United States)

    Ugarte, Fernando; Sousae, Rebekah; Cinquin, Bertrand; Martin, Eric W.; Krietsch, Jana; Sanchez, Gabriela; Inman, Margaux; Tsang, Herman; Warr, Matthew; Passegué, Emmanuelle; Larabell, Carolyn A.; Forsberg, E. Camilla

    2015-01-01

    Summary Epigenetic regulation serves as the basis for stem cell differentiation into distinct cell types, but it is unclear how global epigenetic changes are regulated during this process. Here, we tested the hypothesis that global chromatin organization affects the lineage potential of stem cells and that manipulation of chromatin dynamics influences stem cell function. Using nuclease sensitivity assays, we found a progressive decrease in chromatin digestion among pluripotent embryonic stem cells (ESCs), multipotent hematopoietic stem cells (HSCs), and mature hematopoietic cells. Quantitative high-resolution microscopy revealed that ESCs contain significantly more euchromatin than HSCs, with a further reduction in mature cells. Increased cellular maturation also led to heterochromatin localization to the nuclear periphery. Functionally, prevention of heterochromatin formation by inhibition of the histone methyltransferase G9A resulted in delayed HSC differentiation. Our results demonstrate global chromatin rearrangements during stem cell differentiation and that heterochromatin formation by H3K9 methylation regulates HSC differentiation. PMID:26489895

  5. DOG1 (clone K9) is seldom expressed and not useful in the evaluation of pancreatic neoplasms.

    Science.gov (United States)

    Hemminger, Jessica; Marsh, William L; Iwenofu, Obiajulu Hans; Frankel, Wendy L

    2012-07-01

    DOG1, a transmembrane calcium-regulated chloride channel protein, is a sensitive and specific marker for gastrointestinal stromal tumors compared with other spindle cell and epithelioid neoplasms. Overexpression has also been described in a variety of both benign and malignant epithelial neoplasms. Recently, DOG1 immunoreactivity has been reported in pancreatic solid pseudopapillary tumors (SPT), suggesting a role as a marker for SPT. Utilizing immunohistochemistry, we evaluated DOG1 expression in pancreatic neoplasms to determine the prevalence of staining and establish diagnostic utility. Multiple tissue microarrays (TMA) were created from cores of formalin-fixed paraffin-embedded blocks containing pancreatic adenocarcinomas (n=112), neuroendocrine tumors (n=99), serous cystadenomas (n=28), and SPT (n=14) as well as normal pancreas (n=12). Immunoreactivity for DOG1 (clone K9) was assessed for intensity (1 to 3+), percentage of tumor positivity and location. Of the 99 cases of neuroendocrine tumors, only 2 (2%) were focally positive. Patchy staining was identified in 8 cases (7%) of adenocarcinoma of 1 to 2+ intensity, involving 15% to 80% of the tumor cells and primarily seen in a membranous and luminal distribution. In contrast to a previous report, no DOG1 positivity was observed in SPT, evaluated by both TMA and full sections. The TMAs of serous cystadenomas and normal pancreas were negative for DOG1. Rarely, pancreatic islets displayed granular, cytoplasmic staining. DOG1 antibody clone K9 is not a useful marker for SPT or other primary pancreatic neoplasms. Additional studies may be helpful to evaluate differences between clones of DOG1.

  6. Protein complex interactor analysis and differential activity of KDM3 subfamily members towards H3K9 methylation.

    Directory of Open Access Journals (Sweden)

    Michael Brauchle

    Full Text Available Histone modifications play an important role in chromatin organization and gene regulation, and their interpretation is referred to as epigenetic control. The methylation levels of several lysine residues in histone tails are tightly controlled, and JmjC domain-containing proteins are one class of broadly expressed enzymes catalyzing methyl group removal. However, several JmjC proteins remain uncharacterized, gaps persist in understanding substrate recognition, and the integration of JmjC proteins into signaling pathways is just emerging. The KDM3 subfamily is an evolutionarily conserved group of histone demethylase proteins, thought to share lysine substrate specificity. Here we use a systematic approach to compare KDM3 subfamily members. We show that full-length KDM3A and KDM3B are H3K9me1/2 histone demethylases whereas we fail to observe histone demethylase activity for JMJD1C using immunocytochemical and biochemical approaches. Structure-function analyses revealed the importance of a single amino acid in KDM3A implicated in the catalytic activity towards H3K9me1/2 that is not conserved in JMJD1C. Moreover, we use quantitative proteomic analyses to identify subsets of the interactomes of the 3 proteins. Specific interactor candidates were identified for each of the three KDM3 subfamily members. Importantly, we find that SCAI, a known transcriptional repressor, interacts specifically with KDM3B. Taken together, we identify substantial differences in the biology of KDM3 histone demethylases, namely enzymatic activity and protein-protein interactions. Such comparative approaches pave the way to a better understanding of histone demethylase specificity and protein function at a systems level and are instrumental in identifying the more subtle differences between closely related proteins.

  7. STS-47 Crew Briefing

    Science.gov (United States)

    1992-01-01

    The crew of STS-47, Commander Robert L. Gibson, Pilot Curtis L. Brown, Payload Commander Mark C. Lee, Mission Specialists N. Jan Davis, Jay Apt, and Mae C. Jemison, and Payload Specialist Mamoru Mohri answer questions from the press about the upcoming Endeavour mission and the crew's personal views of the mission.

  8. STS-8 Mission Insignia

    Science.gov (United States)

    1983-01-01

    The eighth flight of the United States Space Transportation System (STS) is represented by eight stars of the constellation Aquila, The Eagle. The Space Shuttle Challenger is pictured affixed the external tank while leaving Earth's atmosphere. Surrounding the outside boder are the names of the crew members.

  9. ns脉冲激光作用下K9玻璃的等离子体谱图研究

    Institute of Scientific and Technical Information of China (English)

    刘丽炜; 蔡红星; 李昌立; 何双力; 张喜和; 冯锴

    2008-01-01

    建立一套激光等离子体光谱测量实验装置,以Nd:YAG调Q倍频固体激光器为激发光源,作用K9玻璃。通过对K9的等离子体谱图的分析发现,K9玻璃等离子体谱强度的变化与激光器输出能量呈线性关系。同时利用显微镜观察K9玻璃的表面损伤形貌,并进行理论分析。

  10. Effect of inclusion matrix model on temperature and thermal stress fields of K9-glass damaged by long-pulse laser

    Science.gov (United States)

    Pan, Yunxiang; Wang, Bin; Shen, Zhonghua; Lu, Jian; Ni, Xiaowu

    2013-04-01

    A model containing an inclusion matrix heated by a millisecond laser is proposed to calculate temperature and thermal stress fields of K9-glass using a finite element method. First, the evolution of temperature and thermal stress fields is analyzed. Results show that both the upper and lower surfaces are damaged. K9-glass is primarily damaged by the combination of radial and axial stresses. Calculated damage morphology is mainly determined by radial stress. Then damage morphology evolution with the increase of the incident laser energy is investigated, which shows that damage area spreads inward from both the front and rear surfaces. Finally, experimental results of long-pulse laser damage of K9-glass are analyzed. The comparison of numerical results with experimental observations shows a good correlation in damage morphology, which indicates that the built inclusion matrix model is applicable to long-pulse laser damage in K9-glass.

  11. Excel与MapGis K9相结合在山东县级林地保护利用规划中的应用

    Institute of Scientific and Technical Information of China (English)

    张晓红; 袁孟妍; 刘国群

    2016-01-01

    MapGis K9在山东省县级林地保护利用规划小班勾绘过程中操作简单,但在小班属性输入时却费时费力。本文通过excel与MapGis K9相结合,批量导入小班属性,大大提高了小班制图的效率。

  12. HP1 recruits activity-dependent neuroprotective protein to H3K9me3 marked pericentromeric heterochromatin for silencing of major satellite repeats.

    Directory of Open Access Journals (Sweden)

    Kerstin Mosch

    Full Text Available H3 lysine 9 trimethylation (H3K9me3 is a histone posttranslational modification (PTM that has emerged as hallmark of pericentromeric heterochromatin. This constitutive chromatin domain is composed of repetitive DNA elements, whose transcription is differentially regulated. Mammalian cells contain three HP1 proteins, HP1α, HP1β and HP1γ These have been shown to bind to H3K9me3 and are thought to mediate the effects of this histone PTM. However, the mechanisms of HP1 chromatin regulation and the exact functional role at pericentromeric heterochromatin are still unclear. Here, we identify activity-dependent neuroprotective protein (ADNP as an H3K9me3 associated factor. We show that ADNP does not bind H3K9me3 directly, but that interaction is mediated by all three HP1 isoforms in vitro. However, in cells ADNP localization to areas of pericentromeric heterochromatin is only dependent on HP1α and HP1β. Besides a PGVLL sequence patch we uncovered an ARKS motif within the ADNP homeodomain involved in HP1 dependent H3K9me3 association and localization to pericentromeric heterochromatin. While knockdown of ADNP had no effect on HP1 distribution and heterochromatic histone and DNA modifications, we found ADNP silencing major satellite repeats. Our results identify a novel factor in the translation of H3K9me3 at pericentromeric heterochromatin that regulates transcription.

  13. An H3K9/S10 methyl-phospho switch modulates Polycomb and Pol II binding at repressed genes during differentiation.

    Science.gov (United States)

    Sabbattini, Pierangela; Sjoberg, Marcela; Nikic, Svetlana; Frangini, Alberto; Holmqvist, Per-Henrik; Kunowska, Natalia; Carroll, Tom; Brookes, Emily; Arthur, Simon J; Pombo, Ana; Dillon, Niall

    2014-03-01

    Methylated histones H3K9 and H3K27 are canonical epigenetic silencing modifications in metazoan organisms, but the relationship between the two modifications has not been well characterized. H3K9me3 coexists with H3K27me3 in pluripotent and differentiated cells. However, we find that the functioning of H3K9me3 is altered by H3S10 phosphorylation in differentiated postmitotic osteoblasts and cycling B cells. Deposition of H3K9me3/S10ph at silent genes is partially mediated by the mitogen- and stress-activated kinases (MSK1/2) and the Aurora B kinase. Acquisition of H3K9me3/S10ph during differentiation correlates with loss of paused S5 phosphorylated RNA polymerase II, which is present on Polycomb-regulated genes in embryonic stem cells. Reduction of the levels of H3K9me3/S10ph by kinase inhibition results in increased binding of RNAPIIS5ph and the H3K27 methyltransferase Ezh1 at silent promoters. Our results provide evidence of a novel developmentally regulated methyl-phospho switch that modulates Polycomb regulation in differentiated cells and stabilizes repressed states.

  14. The Daxx/Atrx Complex Protects Tandem Repetitive Elements during DNA Hypomethylation by Promoting H3K9 Trimethylation.

    Science.gov (United States)

    He, Quanyuan; Kim, Hyeung; Huang, Rui; Lu, Weisi; Tang, Mengfan; Shi, Fengtao; Yang, Dong; Zhang, Xiya; Huang, Junjiu; Liu, Dan; Songyang, Zhou

    2015-09-03

    In mammals, DNA methylation is essential for protecting repetitive sequences from aberrant transcription and recombination. In some developmental contexts (e.g., preimplantation embryos) DNA is hypomethylated but repetitive elements are not dysregulated, suggesting that alternative protection mechanisms exist. Here we explore the processes involved by investigating the role of the chromatin factors Daxx and Atrx. Using genome-wide binding and transcriptome analysis, we found that Daxx and Atrx have distinct chromatin-binding profiles and are co-enriched at tandem repetitive elements in wild-type mouse ESCs. Global DNA hypomethylation further promoted recruitment of the Daxx/Atrx complex to tandem repeat sequences, including retrotransposons and telomeres. Knockdown of Daxx/Atrx in cells with hypomethylated genomes exacerbated aberrant transcriptional de-repression of repeat elements and telomere dysfunction. Mechanistically, Daxx/Atrx-mediated repression seems to involve Suv39h recruitment and H3K9 trimethylation. Our data therefore suggest that Daxx and Atrx safeguard the genome by silencing repetitive elements when DNA methylation levels are low.

  15. The Floating Ampersand: STS Past and STS to Come

    Directory of Open Access Journals (Sweden)

    Sheila Jasanoff

    2016-07-01

    Full Text Available STS has become a discipline in the sense that it offers new ways to read and make sense of the world. It remains an amalgam, however, of two linked yet separate lines of inquiry, both abbreviated as STS. Science and technology studies refers to the investigation of S&T as social institutions; science, technology and society, by contrast, analyzes the external relations of S&T with other institutions, such as law or politics. This essay reflects on the implications of this ambiguity for institutionalizing STS as a field of its own, drawing on the author’s experiences in building STS at two universities.

  16. STS-83 Mission Insignia

    Science.gov (United States)

    1997-01-01

    The crew patch for NASA's STS-83 mission depicts the Space Shuttle Columbia launching into space for the first Microgravity Sciences Laboratory 1 (MSL-1) mission. MSL-1 investigated materials science, fluid dynamics, biotechnology, and combustion science in the microgravity environment of space, experiments that were conducted in the Spacelab Module in the Space Shuttle Columbia's cargo bay. The center circle symbolizes a free liquid under microgravity conditions representing various fluid and materials science experiments. Symbolic of the combustion experiments is the surrounding starburst of a blue flame burning in space. The 3-lobed shape of the outermost starburst ring traces the dot pattern of a transmission Laue photograph typical of biotechnology experiments. The numerical designation for the mission is shown at bottom center. As a forerunner to missions involving International Space Station (ISS), STS-83 represented the hope that scientific results and knowledge gained during the flight will be applied to solving problems on Earth for the benefit and advancement of humankind.

  17. A science teacher's reflections and knowledge growth about STS instruction after actual implementation

    Science.gov (United States)

    Tsai, Chin-Chung

    2002-01-01

    The major purpose of this study was to describe a science teacher's views of STS (Science-Technology-Society) instruction and what she acquired after implementing a two-semester STS-oriented science course in a high school of Taiwan. Upon analysis of the teacher's journals, interview data, concept maps, and relevant student questionnaire responses, this study revealed the following findings. The teacher believed that STS instruction was a potential way of practicing so-called constructivist teaching and her pedagogical knowledge about STS showed a considerable growth. As a result of STS instruction, her epistemological views of science seemed to progress toward more constructivist-oriented views of science. The heavy content load of Taiwan's national curriculum, regular cross-class standard tests, the lack of peers' or administrative support, the resource limitations in Chinese language, and the cultural impacts were identified as major factors that inhibited her implementation of STS instruction.

  18. Situating STS and Thinking Ahead

    Directory of Open Access Journals (Sweden)

    Adele E. Clarke

    2016-07-01

    Full Text Available This essay sketches some of the broader sociopolitical and academic conditions of possibility at the time of the founding of the SKAT Section of the American sociological Association, noting the diversity of concerned groups and the affordances of epistemological diversity. I situate my own development as an early STS scholar, and discuss the growing salience of (postcolonial STS and concerns regarding current STS training and publishing.

  19. Rotenone affects p53 transcriptional activity and apoptosis via targeting SIRT1 and H3K9 acetylation in SH-SY5Y cells.

    Science.gov (United States)

    Feng, Ya; Liu, Te; Dong, Su-Yan; Guo, Yan-Jie; Jankovic, Joseph; Xu, Huaxi; Wu, Yun-Cheng

    2015-08-01

    The protein deacetylase SIRT1 has been recognized to exert its protective effect by directly deacetylasing histone and many other transcriptional factors including p53. However, the effect of SIRT1 on p53 expression at the transcriptional level still remains to be elucidated. In this study, we found that rotenone treatment decreased cell viability, induced apoptosis, reduced SIRT1 level, and promoted p53 expression. Pre-treatment with resveratrol, a SIRT1 activator, could attenuate rotenone-induced cell injury and p53 expression, whereas down-regulation of SIRT1 directly increased p53 expression. Moreover, chromatin immunoprecipitation experiments showed that SIRT1 bound to H3K9 within the p53 promoter region, and this binding resulted in decreased H3K9 acetylation and increased H3K9 tri-methylation, thereby inhibiting p53 gene transcription. In conclusion, our data indicate that rotenone promotes p53 transcription and apoptosis through targeting SIRT1 and H3K9. This leads to nigrostriatal degeneration, the main pathogenic mechanism of motor features of Parkinson's disease. SIRT1, a deacetylase enzyme, has neuroprotective effects for Parkinson's disease via targeting various factors. Resveratrol activated SIRT1 can target H3K9 and regulate p53 gene expression at the transcriptional level, thus inhibiting p53 transcription to enhance neuroprotection, alleviating rotenone induced dopaminergic neurodegeneration. We think these findings should provide a new strategy for the treatment of Parkinson's disease.

  20. The histone H3K9 methylation and RNAi pathways regulate normalnucleolar and repeated DNA organization by inhibiting formation ofextrachromosomal DNAs

    Energy Technology Data Exchange (ETDEWEB)

    Peng, Jamy C.; Karpen, Gary H.

    2006-06-15

    In order to identify regulators of nuclear organization, Drosophila mutants in the Su(var)3-9 histone H3K9 methyltransferase, RNAi pathway components, and other regulators of heterochromatin-mediated gene silencing were examined for altered nucleoli and positioning of repeated DNAs. Animals lacking components of the H3K9 methylation and RNAi pathways contained disorganized nucleoli, ribosomal DNA (rDNA) and satellite DNAs. The levels of H3K9 dimethylation (H3K9me2) in chromatin associated with repeated DNAs decreased dramatically in Su(var)3-9 and dcr-2 (dicer-2) mutant tissues compared to wild type. We also observed a substantial increase in extrachromosomal repeated DNAs in mutant tissues. The disorganized nucleolus phenotype depends on the presence of Ligase 4 (Lig4), and ecc DNA formation is not induced by removal of cohesin. We conclude that H3K9 methylation of rDNA and satellites, maintained by Su(var)3-9, HP1, and the RNAi pathway, is necessary for the structural stability of repeated DNAs, which is mediated through suppression of non-homologous end joining (NHEJ). These results suggest a mechanism for how local chromatin structure can regulate genome stability, and the organization of chromosomal elements and nuclear organelles.

  1. Ubiquitination of Lysine 867 of the Human SETDB1 Protein Upregulates Its Histone H3 Lysine 9 (H3K9) Methyltransferase Activity

    Science.gov (United States)

    Ishimoto, Kenji; Kawamata, Natsuko; Uchihara, Yoshie; Okubo, Moeka; Fujimoto, Reiko; Gotoh, Eiko; Kakinouchi, Keisuke; Mizohata, Eiichi; Hino, Nobumasa; Okada, Yoshiaki; Mochizuki, Yasuhiro; Tanaka, Toshiya; Hamakubo, Takao; Sakai, Juro; Kodama, Tatsuhiko; Inoue, Tsuyoshi; Tachibana, Keisuke; Doi, Takefumi

    2016-01-01

    Posttranslational modifications (PTMs) of proteins play a crucial role in regulating protein-protein interactions, enzyme activity, subcellular localization, and stability of the protein. SET domain, bifurcated 1 (SETDB1) is a histone methyltransferase that regulates the methylation of histone H3 on lysine 9 (H3K9), gene silencing, and transcriptional repression. The C-terminal region of SETDB1 is a key site for PTMs, and is essential for its enzyme activity in mammalian and insect cells. In this study, we aimed to evaluate more precisely the effect of PTMs on the H3K9 methyltransferase activity of SETDB1. Using mass spectrometry analysis, we show that the C-terminal region of human SETDB1 purified from insect cells is ubiquitinated. We also demonstrate that the ubiquitination of lysine 867 of the human SETDB1 is necessary for full H3K9 methyltransferase activity in mammalian cells. Finally, we show that SETDB1 ubiquitination regulates the expression of its target gene, serpin peptidase inhibitor, clade E, member 1 (SERPINE1) by methylating H3K9. These results suggest that the ubiquitination of SETDB1 at lysine 867 controls the expression of its target gene by activating its H3K9 methyltransferase activity. PMID:27798683

  2. Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3-K9.

    Science.gov (United States)

    Tachibana, Makoto; Ueda, Jun; Fukuda, Mikiko; Takeda, Naoki; Ohta, Tsutomu; Iwanari, Hiroko; Sakihama, Toshiko; Kodama, Tatsuhiko; Hamakubo, Takao; Shinkai, Yoichi

    2005-04-01

    Histone H3 Lys 9 (H3-K9) methylation is a crucial epigenetic mark for transcriptional silencing. G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regions and is essential for murine embryogenesis. There is a single G9a-related methyltransferase in mammals, called GLP/Eu-HMTase1. Here we show that GLP is also important for H3-K9 methylation of mouse euchromatin. GLP-deficiency led to embryonic lethality, a severe reduction of H3-K9 mono- and dimethylation, the induction of Mage-a gene expression, and HP1 relocalization in embryonic stem cells, all of which were phenotypes of G9a-deficiency. Furthermore, we show that G9a and GLP formed a stoichiometric heteromeric complex in a wide variety of cell types. Biochemical analyses revealed that formation of the G9a/GLP complex was dependent on their enzymatic SET domains. Taken together, our new findings revealed that G9a and GLP cooperatively exert H3-K9 methyltransferase function in vivo, likely through the formation of higher-order heteromeric complexes.

  3. Inhibition of H3K9 methyltransferase G9a induces autophagy and apoptosis in oral squamous cell carcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Ren, Aishu; Qiu, Yu [Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing Medical University, Chongqing, 401147 (China); Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing, 401147 (China); Cui, Hongjuan [State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716 (China); Fu, Gang, E-mail: fg.ras@hotmail.com [Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing Medical University, Chongqing, 401147 (China); Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing, 401147 (China)

    2015-03-27

    Objective: To explore whether inhibition of H3K9 Methyltransferase G9a could exert an antitumoral effect in oral squamous cell carcinoma (OSCC). Materials and methods: First we checked G9a expression in two OSCC cell lines Tca8113 and KB. Next we used a special G9a inhibitor BIX01294 (BIX) to explore the effect of inhibition of G9a on OSCC in vitro. Cell growth was tested by typlan blue staining, MTT assay and Brdu immunofluorescence staining. Cell autophagy was examined by monodansylcadaverine (MDC) staining, LC3-II immunofluorescence staining and LC3-II western blot assay. Cell apoptosis was checked by FITC Annexin-V and PI labeling, tunnel staining and caspase 3 western blot assay. Finally, the effect of inhibition of G9a on clonogenesis and tumorigenesis capacity of OSCC was analyzed by soft agar growth and xenograft model. Results: Here we showed that G9a was expressed in both Tca8113 and KB cells. Inhibition of G9a using BIX significantly reduced cell growth and proliferation in Tca8113 and KB. Inhibition of G9a induced cell autophagy with conversion of LC3-I to LC3-II and cell apoptosis with the expression of cleaved caspase 3. We also found that inhibition of G9a reduced colony formation in soft agar and repressed tumor growth in mouse xenograph model. Conclusion: Our results suggested that G9a might be a potential epigenetic target for OSCC treatment. - Highlights: • Inhibition of G9a reduced cell growth and proliferation in OSCC cells. • Inhibition of G9a induces autophagy and apoptosis in OSCC cells. • Inhibition of G9a repressed tumor growth in mouse xenograph model.

  4. Dose- and time- effect responses of DNA methylation and histone H3K9 acetylation changes induced by traffic-related air pollution

    Science.gov (United States)

    Ding, Rui; Jin, Yongtang; Liu, Xinneng; Ye, Huaizhuang; Zhu, Ziyi; Zhang, Yuan; Wang, Ting; Xu, Yinchun

    2017-01-01

    As an important risk factor of respiratory disorders, traffic-related air pollution (TRAP) has caused extensive concerns. Epigenetic change has been considered a link between TRAP and respiratory diseases. However, the exact effects of TRAP on epigenetic changes are still unclear. Here we investigated the dose- and time- effect responses of TRAP on DNA methylations and H3K9 acetylation (H3K9ac) in both blood and lung tissues of rats. The findings showed that every 1 μg/m3 increase of TRAP components were associated with changes in %5 mC (95% CI) in LINE-1, iNOS, p16CDKN2A, and APC ranging from −0.088% (−0.150, −0.026) to 0.102 (0.049, 0.154), as well as 0.276 (0.053, 0.498) to 0.475 (0.103, 0.848) ng/mg increase of H3K9ac. In addition, every 1 more day exposure at high level of TRAP (in tunnel) also significantly changed the levels of DNA methylation (ranging from −0.842% to 0.248%) and H3K9ac (16.033 and 15.718 ng/mg pro in PBMC and lung tissue, respectively) changes. Season and/or sex could interact with air pollutants in affecting DNA methylation and H3K9ac. The findings showed that TRAP exposure is dose- and time- dependently associated with the changes of DNA methylation and H3K9ac. PMID:28256616

  5. ATRX binds to atypical chromatin domains at the 3' exons of zinc finger genes to preserve H3K9me3 enrichment.

    Science.gov (United States)

    Valle-García, David; Qadeer, Zulekha A; McHugh, Domhnall S; Ghiraldini, Flávia G; Chowdhury, Asif H; Hasson, Dan; Dyer, Michael A; Recillas-Targa, Félix; Bernstein, Emily

    2016-06-02

    ATRX is a SWI/SNF chromatin remodeler proposed to govern genomic stability through the regulation of repetitive sequences, such as rDNA, retrotransposons, and pericentromeric and telomeric repeats. However, few direct ATRX target genes have been identified and high-throughput genomic approaches are currently lacking for ATRX. Here we present a comprehensive ChIP-sequencing study of ATRX in multiple human cell lines, in which we identify the 3' exons of zinc finger genes (ZNFs) as a new class of ATRX targets. These 3' exonic regions encode the zinc finger motifs, which can range from 1-40 copies per ZNF gene and share large stretches of sequence similarity. These regions often contain an atypical chromatin signature: they are transcriptionally active, contain high levels of H3K36me3, and are paradoxically enriched in H3K9me3. We find that these ZNF 3' exons are co-occupied by SETDB1, TRIM28, and ZNF274, which form a complex with ATRX. CRISPR/Cas9-mediated loss-of-function studies demonstrate (i) a reduction of H3K9me3 at the ZNF 3' exons in the absence of ATRX and ZNF274 and, (ii) H3K9me3 levels at atypical chromatin regions are particularly sensitive to ATRX loss compared to other H3K9me3-occupied regions. As a consequence of ATRX or ZNF274 depletion, cells with reduced levels of H3K9me3 show increased levels of DNA damage, suggesting that ATRX binds to the 3' exons of ZNFs to maintain their genomic stability through preservation of H3K9me3.

  6. STS-3 medical report

    Science.gov (United States)

    Pool, S. L. (Editor); Johnson, P. C., Jr. (Editor); Mason, J. A. (Editor)

    1982-01-01

    The medical operations report for STS-3, which includes a review of the health of the crew before, during, and immediately after the third Shuttle orbital flight is presented. Areas reviewed include: health evaluation, medical debriefing of crewmembers, health stabilization program, medical training, medical 'kit' carried in flight, tests and countermeasures for space motion sickness, cardiovascular profile, biochemistry and endocrinology results, hematology and immunology analyses, medical microbiology, food and nutrition, potable water, shuttle toxicology, radiological health, and cabin acoustic noise. Environmental effects of shuttle launch and landing medical information management, and management, planning, and implementation of the medical program are also dicussed.

  7. STS-65 Mission Insignia

    Science.gov (United States)

    1994-01-01

    Designed by the mission crew members, the STS-65 insignia features the International Microgravity Lab (IML)-2 mission and its Spacelab module which flew aboard the Space Shuttle Columbia. IML-2 is reflected in the emblem by two gold stars shooting toward the heavens behind the IML lettering. The Space Shuttle Columbia is depicted orbiting the logo and reaching off into space, with Spacelab on an international quest for a better understanding of the effects of space flight on materials processing and life sciences.

  8. STS as science or politics?

    Science.gov (United States)

    Collins, Harry; Evans, Robert; Weinel, Martin

    2017-08-01

    In a recent editorial for this journal, Sergio Sismondo makes two claims. First, he states that STS bears no responsibility for the emergence of post-truth politics. Second, he claims that debates about the nature of expertise that take place within STS are irrelevant in this context. In contrast, we argue that, whether or not STS had a causal influence on the emergence of post-truth politics, there is a clear resonance between the two positions and that the current political climate makes the empirically informed and scientific analysis of expertise and the form of life of science more important than ever. We argue that treating the contribution of STS to these matters as essentially political rather than scientific surrenders any special role we have as experts on the organization and values of science and leaves STS as just one political actor among others.

  9. MapGIS K9投影变换在国土资源“一张图”中的应用研究%Research on the Apptication of MapGIS k9 Projection Transformation in Lamd Resources A Map

    Institute of Scientific and Technical Information of China (English)

    钟世彬; 胡水平; 闫喜凤

    2011-01-01

    This paper firmly introduced the I luir*ctrri*lic? Of MapOIS K9 projection tranaformalion. "Own il foruiwd on the application c the coordinate import,coordinate transformation,and dynamic projection in I.mil Resource* A Map. It was proved tli.it MapGIS K9 can improve efficiency and quality of Land Resourcr* A Mup.%介绍了MapGIS K9投影变换特点,重点阐述了坐标导入、坐标特换和动态投影在国土资源“一张图”中的应用实例.通过试验验证,MapGIS K9有利于提高国土资源“一张国”工程建设效率与质量.

  10. ANSYS在脉冲CO2激光对K9玻璃损伤过程模拟中的应用研究%Application of ANSYS in Simulation Process of Pulse CO2 Laser Damage of K9 Glass

    Institute of Scientific and Technical Information of China (English)

    王玺; 李化; 聂劲松

    2008-01-01

    从脉冲CO2激光损伤K9玻璃有限元模型的建立和网格划分、脉冲激光的加载、设置初始条件和边界条件等几个方面,分析了基于ANSYS的脉冲CO2激光损伤K9玻璃模拟过程中的一些关键问题,建立了脉冲CO2激光损伤K9玻璃的有限元模型,对K9玻璃受到脉冲CO2激光辐照时的温度和应力分布进行了数值分析.

  11. KMT1E mediated H3K9 methylation is required for the maintenance of embryonic stem cells by repressing trophectoderm differentiation.

    Science.gov (United States)

    Lohmann, Felix; Loureiro, Joseph; Su, Hui; Fang, Qing; Lei, Hong; Lewis, Tanya; Yang, Yi; Labow, Mark; Li, En; Chen, Taiping; Kadam, Shilpa

    2010-02-01

    Dynamic regulation of histone methylation by methyltransferases and demethylases plays a central role in regulating the fate of embryonic stem (ES) cells. The histone H3K9 methyltransferase KMT1E, formerly known as ESET or Setdb1, is essential to embryonic development as the ablation of the Setdb1 gene results in peri-implantation lethality and prevents the propagation of ES cells. However, Setdb1-null blastocysts do not display global changes in H3K9 methylation or DNA methylation, arguing against a genome-wide defect. Here we show that conditional deletion of the Setdb1 gene in ES cells results in the upregulation of lineage differentiation markers, especially trophectoderm-specific factors, similar to effects observed upon loss of Oct3/4 expression in ES cells. We demonstrate that KMT1E deficiency in ES cells leads to a decrease in histone H3K9 methylation at and derepression of trophoblast-associated genes such as Cdx2. Furthermore, we find genes that are derepressed upon Setdb1 deletion to overlap with known targets of polycomb mediated repression, suggesting that KMT1E mediated H3K9 methylation acts in concert with polycomb controlled H3K27 methylation. Our studies thus demonstrate an essential role for KMT1E in the control of developmentally regulated gene expression programs in ES cells.

  12. Continual removal of H3K9 promoter methylation by Jmjd2 demethylases is vital for ESC self-renewal and early development

    DEFF Research Database (Denmark)

    Pedersen, Marianne Terndrup; Kooistra, Susanne Marije; Radzisheuskaya, Aliaksandra

    2016-01-01

    Chromatin-associated proteins are essential for the specification and maintenance of cell identity. They exert these functions through modulating and maintaining transcriptional patterns. To elucidate the functions of the Jmjd2 family of H3K9/H3K36 histone demethylases, we generated conditional J...... demethylases represents a novel mechanism ensuring transcriptional competence and stability of the pluripotent cell identity....

  13. The ATRX-ADD domain binds to H3 tail peptides and reads the combined methylation state of K4 and K9

    Science.gov (United States)

    Dhayalan, Arunkumar; Tamas, Raluca; Bock, Ina; Tattermusch, Anna; Dimitrova, Emilia; Kudithipudi, Srikanth; Ragozin, Sergey; Jeltsch, Albert

    2011-01-01

    Mutations in the ATRX protein are associated with the alpha-thalassemia and mental retardation X-linked syndrome (ATR-X). Almost half of the disease-causing mutations occur in its ATRX-Dnmt3-Dnmt3L (ADD) domain. By employing peptide arrays, chromatin pull-down and peptide binding assays, we show specific binding of the ADD domain to H3 histone tail peptides containing H3K9me3. Peptide binding was disrupted by the presence of the H3K4me3 and H3K4me2 modification marks indicating that the ATRX-ADD domain has a combined readout of these two important marks (absence of H3K4me2 and H3K4me3 and presence of H3K9me3). Disease-causing mutations reduced ATRX-ADD binding to H3 tail peptides. ATRX variants, which fail in the H3K9me3 interaction, show a loss of heterochromatic localization in cells, which indicates the chromatin targeting function of the ADD domain of ATRX. Disruption of H3K9me3 binding may be a general pathogenicity pathway of ATRX mutations in the ADD domain which may explain the clustering of disease mutations in this part of the ATRX protein. PMID:21421568

  14. 固结磨料化学机械研抛K9玻璃的材料去除机理

    Institute of Scientific and Technical Information of China (English)

    刘大伟; 巨金艳

    2015-01-01

    固结磨料化学机械磨料研抛K9材料取出的时候,其机械性能和化学作用是相互分离的,同时在处理的过程中对工件表面的硬度也会产生非常重大的影响.相关的研究已经显示研抛液能够和K9玻璃产生非常行明显的化学反应,同时能够起到保护的作用,在去除K9材料的时候是随着浸泡时间的增长而增长的,其去除的主要方式是脆性去除.主要分析了固结磨料化学机械研抛K9玻璃的材料去除机理,以供参考和借鉴.

  15. Biodegradation of malachite green by strain Pseudomonas sp. K9 and cloning of the tmr2 gene associated with an ISPpu12.

    Science.gov (United States)

    Huan-Mei; Lian-Tai, Li; Cai-Fang, Yan; Jin-Jin, Sun; Yuan-Gao; Hong, Qing; Shun-Peng, Li

    2011-06-01

    A bacterial strain K9 capable of degrading malachite green was isolated from the sludge of the wastewater treatment system of a chemical plant. It was identified preliminarily as Pseudomonas sp. Strain K9 was also able to degrade other triphenylmethane dyes, such as Crystal Violet and Basic Fuchsin. The gene tmr2, encoding the triphenylmethane reductase, was cloned from strain K9, and functionally expressed in E. coli. A 5946-bp DNA fragment including the tmr2 gene was cloned from the genomic DNA of strain K9 by chromosome walking. Its sequence analysis showed that tmr2 was associated with a typical mobile element ISPpu12 consisting of tnpA (encoding a transposase), lspA (encoding a lipoprotein signal peptidase) and orf1 (encoding a putative MerR family regulator), orf2 (encoding a CDF family heavy metal/H(+) antiporter). This association was also found in another malachite green-degrading strain Pseudomonas sp. MDB-1, which indicated that the tmr2 gene might be a horizontally transferable gene.

  16. 高丽神话——韩国K9“雷声”自行式榴弹炮

    Institute of Scientific and Technical Information of China (English)

    国武

    2007-01-01

    1999年,韩国陆军装备了自行研制的K9“雷声”(Thunder52倍口径155毫米自行式榴弹炮,成为亚洲第一个、世界第二个正式装备52倍口径155毫米自行榴弹炮的国家。“墙内开花,墙外也香”,除了在2001年8月与土耳其签订的价值10亿美元的K9火炮出口合同外,K9连同法国的“恺撒”及瑞典的FH77已赫然出现在亚太地区国家陆军优先考虑采购的新型重型火炮系统清单中。韩国K9自行式榴弹炮已经冲出亚洲,逐步走向世界了。

  17. 高丽神话——记韩国K9“雷声”自行式榴弹炮

    Institute of Scientific and Technical Information of China (English)

    肖咏捷

    2006-01-01

    1999年,韩国陆军装备了自行研制的K9“雷声”(Thunder)52倍口径155毫米自行式榴弹炮,成为亚洲第一个、世界第二个正式装备52倍口径155毫米自行榴弹炮的国家。墙内开花墙外也香,除了在2001年8月与土耳其签订的价值10亿美元的K9火炮出口合同外,K9连同法国的“凯撒”及瑞典的FH-77已赫然出现在亚太地区国家陆军优先考虑采购的新型重型火炮系统清单中。韩国K9自行式榴弹炮忆经冲出亚洲,逐渐走向世界了。

  18. The ATRX-ADD domain binds to H3 tail peptides and reads the combined methylation state of K4 and K9.

    Science.gov (United States)

    Dhayalan, Arunkumar; Tamas, Raluca; Bock, Ina; Tattermusch, Anna; Dimitrova, Emilia; Kudithipudi, Srikanth; Ragozin, Sergey; Jeltsch, Albert

    2011-06-01

    Mutations in the ATRX protein are associated with the alpha-thalassemia and mental retardation X-linked syndrome (ATR-X). Almost half of the disease-causing mutations occur in its ATRX-Dnmt3-Dnmt3L (ADD) domain. By employing peptide arrays, chromatin pull-down and peptide binding assays, we show specific binding of the ADD domain to H3 histone tail peptides containing H3K9me3. Peptide binding was disrupted by the presence of the H3K4me3 and H3K4me2 modification marks indicating that the ATRX-ADD domain has a combined readout of these two important marks (absence of H3K4me2 and H3K4me3 and presence of H3K9me3). Disease-causing mutations reduced ATRX-ADD binding to H3 tail peptides. ATRX variants, which fail in the H3K9me3 interaction, show a loss of heterochromatic localization in cells, which indicates the chromatin targeting function of the ADD domain of ATRX. Disruption of H3K9me3 binding may be a general pathogenicity pathway of ATRX mutations in the ADD domain which may explain the clustering of disease mutations in this part of the ATRX protein.

  19. Ornithine decarboxylase antizyme induces hypomethylation of genome DNA and histone H3 lysine 9 dimethylation (H3K9me2 in human oral cancer cell line.

    Directory of Open Access Journals (Sweden)

    Daisuke Yamamoto

    Full Text Available BACKGROUND: Methylation of CpG islands of genome DNA and lysine residues of histone H3 and H4 tails regulates gene transcription. Inhibition of polyamine synthesis by ornithine decarboxylase antizyme-1 (OAZ in human oral cancer cell line resulted in accumulation of decarboxylated S-adenosylmethionine (dcSAM, which acts as a competitive inhibitor of methylation reactions. We anticipated that accumulation of dcSAM impaired methylation reactions and resulted in hypomethylation of genome DNA and histone tails. METHODOLOGY/PRINCIPAL FINDINGS: Global methylation state of genome DNA and lysine residues of histone H3 and H4 tails were assayed by Methylation by Isoschizomers (MIAMI method and western blotting, respectively, in the presence or absence of OAZ expression. Ectopic expression of OAZ mediated hypomethylation of CpG islands of genome DNA and histone H3 lysine 9 dimethylation (H3K9me2. Protein level of DNA methyltransferase 3B (DNMT3B and histone H3K9me specific methyltransferase G9a were down-regulated in OAZ transfectant. CONCLUSIONS/SIGNIFICANCE: OAZ induced hypomethylation of CpG islands of global genome DNA and H3K9me2 by down-regulating DNMT3B and G9a protein level. Hypomethylation of CpG islands of genome DNA and histone H3K9me2 is a potent mechanism of induction of the genes related to tumor suppression and DNA double strand break repair.

  20. Suv39h-dependent H3K9me3 marks intact retrotransposons and silences LINE elements in mouse embryonic stem cells.

    Science.gov (United States)

    Bulut-Karslioglu, Aydan; De La Rosa-Velázquez, Inti A; Ramirez, Fidel; Barenboim, Maxim; Onishi-Seebacher, Megumi; Arand, Julia; Galán, Carmen; Winter, Georg E; Engist, Bettina; Gerle, Borbala; O'Sullivan, Roderick J; Martens, Joost H A; Walter, Jörn; Manke, Thomas; Lachner, Monika; Jenuwein, Thomas

    2014-07-17

    Heterochromatin is required to restrict aberrant expression of retrotransposons, but it remains poorly defined due to the underlying repeat-rich sequences. We dissected Suv39h-dependent histone H3 lysine 9 trimethylation (H3K9me3) by genome-wide ChIP sequencing in mouse embryonic stem cells (ESCs). Refined bioinformatic analyses of repeat subfamilies indicated selective accumulation of Suv39h-dependent H3K9me3 at interspersed repetitive elements that cover ∼5% of the ESC epigenome. The majority of the ∼8,150 intact long interspersed nuclear elements (LINEs) and endogenous retroviruses (ERVs), but only a minor fraction of the >1.8 million degenerate and truncated LINEs/ERVs, are enriched for Suv39h-dependent H3K9me3. Transcriptional repression of intact LINEs and ERVs is differentially regulated by Suv39h and other chromatin modifiers in ESCs but governed by DNA methylation in committed cells. These data provide a function for Suv39h-dependent H3K9me3 chromatin to specifically repress intact LINE elements in the ESC epigenome.

  1. Loss of H3K9me3 Correlates with ATM Activation and Histone H2AX Phosphorylation Deficiencies in Hutchinson-Gilford Progeria Syndrome

    Science.gov (United States)

    Zhang, Haoyue; Sun, Linlin; Wang, Kun; Wu, Di; Trappio, Mason; Witting, Celeste; Cao, Kan

    2016-01-01

    Compelling evidence suggests that defective DNA damage response (DDR) plays a key role in the premature aging phenotypes in Hutchinson-Gilford progeria syndrome (HGPS). Studies document widespread alterations in histone modifications in HGPS cells, especially, the global loss of histone H3 trimethylated on lysine 9 (H3K9me3). In this study, we explore the potential connection(s) between H3K9me3 loss and the impaired DDR in HGPS. When cells are exposed to a DNA-damaging agent Doxorubicin (Dox), double strand breaks (DSBs) are generated that result in the phosphorylation of histone H2A variant H2AX (gammaH2AX) within an hour. We find that the intensities of gammaH2AX foci appear significantly weaker in the G0/G1 phase HGPS cells compared to control cells. This reduction is associated with a delay in the recruitment of essential DDR factors. We further demonstrate that ataxia-telangiectasia mutated (ATM) is responsible for the amplification of gammaH2AX signals at DSBs during G0/G1 phase, and its activation is inhibited in the HGPS cells that display significant loss of H3K9me3. Moreover, methylene (MB) blue treatment, which is known to save heterochromatin loss in HGPS, restores H3K9me3, stimulates ATM activity, increases gammaH2AX signals and rescues deficient DDR. In summary, this study demonstrates an early DDR defect of attenuated gammaH2AX signals in G0/G1 phase HGPS cells and provides a plausible connection between H3K9me3 loss and DDR deficiency. PMID:27907109

  2. STS 128 - Christer Fuglesang

    CERN Multimedia

    2009-01-01

    STS-128 (ISS assembly flight 17A) is the next space shuttle mission to the International Space Station (ISS), planned for August 25, 2009. It is expected to use Space Shuttle Discovery. The primary payload will be the Multi-Purpose Logistics Module Leonardo. The mission is scheduled to include three spacewalks to remove and replace a materials processing experiment outside ESA's Columbus module and return an empty ammonia tank assembly As a graduate student, Fuglesang worked at CERN (the European Research Center on Particle Physics) in Geneva on the UA5 experiment, which studied proton-antiproton collisions. In 1988 he became a Fellow of CERN, where he worked on the CPLEAR experiment studying the subtle CP-violation of kaon particles. After a year he became a Senior Fellow and head of the particle identification subdetector........

  3. STS-121 Launch

    Science.gov (United States)

    2006-01-01

    Space Shuttle Discovery and its seven-member crew launched at 2:38 p.m. (EDT) to begin the two-day journey to the International Space Station (ISS) on the historic Return to Flight STS-121 mission. The shuttle made history as it was the first human-occupying spacecraft to launch on Independence Day. During its 12-day mission, this utilization and logistics flight delivered a multipurpose logistics module (MPLM) to the ISS with several thousand pounds of new supplies and experiments. In addition, some new orbital replacement units (ORUs) were delivered and stowed externally on the ISS on a special pallet. These ORUs are spares for critical machinery located on the outside of the ISS. During this mission the crew also carried out testing of Shuttle inspection and repair hardware, as well as evaluated operational techniques and concepts for conducting on-orbit inspection and repair.

  4. STS-40 Mission Insignia

    Science.gov (United States)

    1990-01-01

    The STS-40 patch makes a contemporary statement focusing on human beings living and working in space. Against a background of the universe, seven silver stars, interspersed about the orbital path of Columbia, represent the seven crew members. The orbiter's flight path forms a double-helix, designed to represent the DNA molecule common to all living creatures. In the words of a crew spokesman, ...(the helix) affirms the ceaseless expansion of human life and American involvement in space while simultaneously emphasizing the medical and biological studies to which this flight is dedicated. Above Columbia, the phrase Spacelab Life Sciences 1 defines both the Shuttle mission and its payload. Leonardo Da Vinci's Vitruvian man, silhouetted against the blue darkness of the heavens, is in the upper center portion of the patch. With one foot on Earth and arms extended to touch Shuttle's orbit, the crew feels, he serves as a powerful embodiment of the extension of human inquiry from the boundaries of Earth to the limitless laboratory of space. Sturdily poised amid the stars, he serves to link scentists on Earth to the scientists in space asserting the harmony of efforts which produce meaningful scientific spaceflight missions. A brilliant red and yellow Earth limb (center) links Earth to space as it radiates from a native American symbol for the sun. At the frontier of space, the traditional symbol for the sun vividly links America's past to America's future, the crew states. Beneath the orbiting Shuttle, darkness of night rests peacefully over the United States. Drawn by artist Sean Collins, the STS 40 Space Shuttle patch was designed by the crewmembers for the flight.

  5. STS-107 Master Experiment List

    Science.gov (United States)

    2002-12-01

    A master list of the various experiments conducted aboard the STS-107 Space Mission is presented. The topics include: 1) Biology; 2) Earth and Space Sciences; 3) Physical Sciences; 4) Space Product Development; and 6) Technology Development.

  6. STS-31: APU Controller Removal

    Science.gov (United States)

    1990-01-01

    The launch April 10 of the STS-31 was scrubbed at T-4 minutes due to a faulty valve in auxiliary power unit (APU) number one. The auxiliary power unit is a hydrazine-fueled, turbine-driven power unit that generates mechanical shaft power to drive a hydraulic pump that produces pressure for the orbiter's hydraulic system. This video shows the removal of the STS-31's auxiliary power unit (APU).

  7. Optimization of K9 Glass Film as Protection for Erbium-Doped Phosphate Glass Surface during Ion-Exchange Processing%K9玻璃薄膜对离子交换磷酸盐玻璃波导表面保护的优化研究

    Institute of Scientific and Technical Information of China (English)

    韩秀友; 初凤红; 蔡海文; 方祖捷; 赵明山

    2008-01-01

    采用镀K9玻璃薄膜方法来解决离子交换掺铒磷酸盐玻璃波导表面的侵蚀问题,对K9玻璃薄膜的厚度进行了优化研究.测量分析了样品的荧光光谱和荧光寿命,采用光学显微镜和棱镜耦合技术对不同K9玻璃薄膜厚度下制备波导的表面形貌和导光特性进行了表征和测试.结果表明,与掺铒磷酸盐玻璃原材料相比,镀K9玻璃薄膜后荧光光谱保持不变,荧光寿命稍有下降(约0.2 ms);K9玻璃薄膜的厚度在60~80 nm的范围内保护效果最佳.为下一步制备掺铒有源玻璃光波导器件奠定了良好的实验基础.

  8. The role of Ser54 in the antinociceptive activity of BmK9, a neurotoxin from the scorpion Buthus martensii Karsch.

    Science.gov (United States)

    Wang, Yueqiu; Hao, Zhihui; Shao, Jianhua; Song, Yongbo; Li, Chunli; Li, Chuan; Zhao, Yongshan; Liu, Yanfeng; Wei, Tingting; Wu, Chunfu; Zhang, Jinghai

    2011-11-01

    Residue 54 has been shown to be important for bioactivity in several toxins. However, its role in the antinociceptive activity of toxins has not been evaluated yet. In this study, site-directed mutagenesis and mouse acetic acid writhing test were used to investigate the role of Ser54 in the antinociceptive activity of BmK9 neurotoxin from the Buthus martensii Karsch scorpion. Detailed mutagenesis analysis revealed that substitution of Ser54 by various polar amino acids produced no significant change in the antinociceptive activity, while all substitutions of nonpolar amino acid for Ser54 led to a significant loss of antinociceptive activity. Following the conformational analysis, it was suggested that Ser54 in BmK9 plays a functional role in the antinociceptive activity, the residue exerts its effect by means of a side-chain hydrogen bond.

  9. Catalytic roles of lysines (K9, K27, K31) in the N-terminal domain in human adenylate kinase by random site-directed mutagenesis.

    Science.gov (United States)

    Ayabe, T; Park, S K; Takenaka, H; Sumida, M; Uesugi, S; Takenaka, O; Hamada, M

    1996-11-01

    To elucidate lysine residues in the N-terminal domain of human cytosolic adenylate kinase (hAK1, EC 2.7.4.3), random site-directed mutagenesis of K9, K27, and K31 residues was performed, and six mutants were analyzed by steady-state kinetics. K9 residue may play an important role in catalysis by interacting with AMP2-. K27 and K31 residues appear to play a functional role in catalysis by interacting with MgATP2-. In human AK, the epsilon-amino group in the side chain of these lysine residues would be essential for phosphoryl transfer between MgATP2- and AMP2- during transition state.

  10. Study of methyl transferase (G9aMT) and methylated histone (H3-K9) expressions in unexplained recurrent spontaneous abortion (URSA) and normal early pregnancy.

    Science.gov (United States)

    Fatima, Nishat; Ahmed, S H; Salhan, Sudha; Rehman, S M F; Kaur, Jatinder; Owais, M; Chauhan, Shyam S

    2011-11-01

    We investigated the expression of methyl transferase G9a and methylated histone H3-K9 in fresh human decidual/endometrial tissue of 12 normal early pregnancies and 15 unexplained recurrent spontaneous abortions (URSA). The samples were obtained through dilatation and curettage and collected as per strict inclusion-exclusion criteria. The tissue was subjected to immunohistochemical analysis (IHC), western blotting (WB) and RT-PCR analysis. The results demonstrated methyl transferase G9a to have a lower expression in abortions when compared with that in normal pregnancy (P K9 was significantly lower (P < 0.0001) in URSA tissues than in controls. This study suggests that methylation may cause URSA and indicates the need for further work to explore the role of methylation in URSA and its possible prevention through locally acting methylating/demethylating agents.

  11. High-affinity binding of Chp1 chromodomain to K9 methylated histone H3 is required to establish centromeric heterochromatin.

    Science.gov (United States)

    Schalch, Thomas; Job, Godwin; Noffsinger, Victoria J; Shanker, Sreenath; Kuscu, Canan; Joshua-Tor, Leemor; Partridge, Janet F

    2009-04-10

    In fission yeast, assembly of centromeric heterochromatin requires the RITS complex, which consists of Ago1, Tas3, Chp1, and siRNAs derived from centromeric repeats. Recruitment of RITS to centromeres has been proposed to depend on siRNA-dependent targeting of Ago1 to centromeric sequences. Previously, we demonstrated that methylated lysine 9 of histone H3 (H3K9me) acts upstream of siRNAs during heterochromatin establishment. Our crystal structure of Chp1's chromodomain in complex with a trimethylated lysine 9 H3 peptide reveals extensive sites of contact that contribute to Chp1's high-affinity binding. We found that this high-affinity binding is critical for the efficient establishment of centromeric heterochromatin, but preassembled heterochromatin can be maintained when Chp1's affinity for H3K9me is greatly reduced.

  12. ChIP-seq analysis of histone H3K9 trimethylation in peripheral blood mononuclear cells of membranous nephropathy patients

    Energy Technology Data Exchange (ETDEWEB)

    Sui, W.G. [Guangxi Key Laboratory of Metabolic Diseases Research, Nephrology Department, 181st Hospital, Guilin, Guangxi (China); He, H.Y. [The Life Science College, Guangxi Normal University, Guilin, Guangxi (China); Yan, Q.; Chen, J.J. [Guangxi Key Laboratory of Metabolic Diseases Research, Nephrology Department, 181st Hospital, Guilin, Guangxi (China); Zhang, R.H. [The Life Science College, Guangxi Normal University, Guilin, Guangxi (China); Dai, Y. [Clinical Medical Research Center, The Second Clinical Medical College, Shenzhen People’s Hospital, Jinan University, Shenzhen, Guangdong (China)

    2013-12-12

    Membranous nephropathy (MN), characterized by the presence of diffuse thickening of the glomerular basement membrane and subepithelial in situ immune complex disposition, is the most common cause of idiopathic nephrotic syndrome in adults, with an incidence of 5-10 per million per year. A number of studies have confirmed the relevance of several experimental insights to the pathogenesis of human MN, but the specific biomarkers of MN have not been fully elucidated. As a result, our knowledge of the alterations in histone methylation in MN is unclear. We used chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) to analyze the variations in a methylated histone (H3K9me3) in peripheral blood mononuclear cells from 10 MN patients and 10 healthy subjects. There were 108 genes with significantly different expression in the MN patients compared with the normal controls. In MN patients, significantly increased activity was seen in 75 H3K9me3 genes, and decreased activity was seen in 33, compared with healthy subjects. Five positive genes, DiGeorge syndrome critical region gene 6 (DGCR6), sorting nexin 16 (SNX16), contactin 4 (CNTN4), baculoviral IAP repeat containing 3 (BIRC3), and baculoviral IAP repeat containing 2 (BIRC2), were selected and quantified. There were alterations of H3K9me3 in MN patients. These may be candidates to help explain pathogenesis in MN patients. Such novel findings show that H3K9me3 may be a potential biomarker or promising target for epigenetic-based MN therapies.

  13. 基于MapGIS K9的农村集体土地所有权建库研究

    Institute of Scientific and Technical Information of China (English)

    李扬帆

    2014-01-01

    在农村集体土地所有权确权登记发证项目中,需要进行数据库建设。介绍了MapGIS K9平台的功能及数据库建设流程,分析了数据要求及数据检查应该注意的问题。

  14. Final report of the key comparison APMP.QM-K9: APMP comparison on pH measurement of phosphate buffer

    Science.gov (United States)

    Hioki, Akiharu; Ohata, Masaki; Cherdchu, Chainarong; Tangpaisarnkul, Nongluck

    2011-01-01

    The APMP.QM-K9 was organised by TCQM of APMP to test the abilities of the national metrology institutes in the APMP region to measure a pH value of a phosphate buffer. This APMP comparison on pH measurement was proposed by the National Metrology Institute of Japan, NMIJ, and the National Institute of Metrology of Thailand, NIMT, in August 2009. After approval by TCQM, the comparison has been conducted by NMIJ and NIMT. The comparison is a key comparison following CCQM-K9, CCQM-K9.1 and CCQM-K9.2. The comparison material was a phosphate buffer of pH around 6.86 and the measurement temperatures were 15 °C, 25 °C and 37 °C. This is the first APMP key comparison on pH measurement and the third APMP comparison on pH measurement following APMP.QM-P06 (two phosphate buffers) in 2004 and APMP.QM-P09 (a phthalate buffer) in 2006. The results can be used further by any participant to support its CMC claim for a phosphate buffer. That claim will concern the pH method employed by the participant during this comparison and will cover the temperature(s) used or the full temperature range between 15 °C and 37 °C for the participant which measured pH values at the three temperatures. Main text. To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCQM, according to the provisions of the CIPM Mutual Recognition Arrangement (MRA).

  15. Growth of bultfonteinite and hydrogarnet in metasomatized basalt xenoliths in the B/K9 kimberlite, Damtshaa, Botswana: insights into hydrothermal metamorphism in kimberlite pipes

    Science.gov (United States)

    Buse, Ben; Schumacher, John C.; Sparks, R. Stephen J.; Field, Matthew

    2010-10-01

    Metamorphic assemblages within Karoo basalt xenoliths, found within volcaniclastic kimberlite of the B/K9 pipe, Damtshaa, Botswana, constrain conditions of kimberlite alteration. Bultfonteinite and chlorite partially replace the original augite-plagioclase assemblage, driven by the serpentinisation of the kimberlite creating strong chemical potential gradients for Si and Mg. Hydrogarnet and serpentine replace these earlier metamorphic assemblages as the deposits cool. The bultfonteinite (ideally Ca2SiO2[OH,F]4) and hydrogarnet assemblages require a water-rich fluid containing F-, and imply hydrothermal alteration dominated by external fluids rather than autometamorphism from deuteric fluids. Bultfonteinite and hydrogarnet are estimated to form at temperatures of ca. 350-250°C, which are similar to those for serpentinisation. Alteration within the B/K9 kimberlite predominantly occurs between 250 and 400°C. We attribute these conditions to increased efficiency of mass transfer and chemical reactions below the critical point of water and a consequence of volume-increasing serpentinisation and metasomatic reactions that take place over this temperature range. A comparison of the B/K9 kimberlite with kimberlites from Venetia, South Africa suggests that the composition and mineralogy of included xenoliths affects the alteration assemblages within kimberlite deposits.

  16. The SET-domain protein SUVR5 mediates H3K9me2 deposition and silencing at stimulus response genes in a DNA methylation-independent manner.

    Directory of Open Access Journals (Sweden)

    Elena Caro

    Full Text Available In eukaryotic cells, environmental and developmental signals alter chromatin structure and modulate gene expression. Heterochromatin constitutes the transcriptionally inactive state of the genome and in plants and mammals is generally characterized by DNA methylation and histone modifications such as histone H3 lysine 9 (H3K9 methylation. In Arabidopsis thaliana, DNA methylation and H3K9 methylation are usually colocated and set up a mutually self-reinforcing and stable state. Here, in contrast, we found that SUVR5, a plant Su(var3-9 homolog with a SET histone methyltransferase domain, mediates H3K9me2 deposition and regulates gene expression in a DNA methylation-independent manner. SUVR5 binds DNA through its zinc fingers and represses the expression of a subset of stimulus response genes. This represents a novel mechanism for plants to regulate their chromatin and transcriptional state, which may allow for the adaptability and modulation necessary to rapidly respond to extracellular cues.

  17. 喉癌细胞系中抑癌基因MGMT表达与DNA甲基化及组蛋白H3-K9甲基化的关系%Correlation between histone H3-K9 methylation, DNA methylation and expression of gene MGMT in Hep-2 cell line

    Institute of Scientific and Technical Information of China (English)

    杨静; 何丽霞; 季文樾; 金明珠; 赵旭东

    2012-01-01

    To explore the correlation between histone H3-K9 methylation, DNA methylation and expression of carcinoma suppressor gene MGMT in laryngeal carcinoma Hep-2 cell line. Method: 5-Aza-dC was used to deal with Hep-2 cell cultured in vitro. ChIP, MSP and Realtime- PCR were used to detect H3-K9 methylation, DNA methylation, of MGMT gene promoter region and MGMT gene expression before and after treatment with drugs. Result: In Hep-2 cell line,gene MGMT was characterized by DNA methylation and histone H3-K9 hypermethylation. 5-Aza-dC was able to reduce H3-K9 methylation of MGMT gene histone in Hep-2 cell line, 5-Aza-dC was able to reverse DNA methylation of MGMT gene histone in Hep-2 cell line,5-Aza-dC was able to up-regulate the down-regulated gene expression of tumor suppressor genes MGMT. Conclusion: Promoter methylation of cancer suppressor gene MGMT may induce the gene inactivity. DNA methylation may increase H3-K9 methylation. 5-Aza-dC can reduce H3-K9 methylation of tumor suppressor gene MGMT histone by reversing DNA methylation of tumor suppressor gene MGMT, and then the expression of tumor suppressor genes is increased and tumor development is inhibited.%目的:探讨喉癌Hep-2细胞中组蛋白H3-K9甲基化与DNA甲基化及抑癌基因MGMT表达的关系.方法:应用去甲基化制剂5-氮杂-2'-脱氧胞苷(5-Aza-dC)处理体外培养的Hep-2细胞.应用染色质免疫沉淀技术、甲基化特异性聚合酶链反应和实时定量逆转录聚合酶链反应分析药物作用前后MGMT基因启动子区组蛋白H3-K9甲基化、DNA甲基化和MGMT表达情况.结果:①在Hep-2细胞未经药物干预前,MGMT表现为DNA甲基化,组蛋白H3-K9高甲基化,MGMT低表达.②在5-Aza-dC的作用下,Hep-2细胞中MGMT的组蛋白H3-K9甲基化状态被降低;MGMT的DNA甲基化状态得到了逆转;原来低表达的MGMT表达上调.结论:喉癌细胞系中MGMT启动子区甲基化可能是导致其基因失活的主要原因.DNA甲

  18. STS-99 Crew Insignia

    Science.gov (United States)

    1999-01-01

    The STS-99 crew members designed the flight insignia for the Shuttle Radar Topography Mission (SRTM), the most ambitious Earth mapping mission to date. Two radar anternas, one located in the Shuttle bay and the other located on the end of a 60-meter deployable mast, was used during the mission to map Earth's features. The goal was to provide a 3-dimensional topographic map of the world's surface up to the Arctic and Antarctic Circles. In the patch, the clear portion of Earth illustrates the radar beams penetrating its cloudy atmosphere and the unique understanding of the home planet that is provided by space travel. The grid on Earth reflects the mapping character of the SRTM mission. The patch depicts the Space Shuttle Endeavour orbiting Earth in a star spangled universe. The rainbow along Earth's horizon resembles an orbital sunrise. The crew deems the bright colors of the rainbow as symbolic of the bright future ahead because of human beings' venturing into space. The crew of six launched aboard the Space Shuttle Endeavor on February 11, 2000 and completed 222 hours of around the clock radar mapping gathering enough information to fill more than 20,000 CDs.

  19. STS-78 Mission Insignia

    Science.gov (United States)

    1996-01-01

    The STS-78 patch links past with present to tell the story of its mission and science through a design imbued with the strength and vitality of the 2-dimensional art of North America's northwest coast Indians. Central to the design is the space Shuttle whose bold lines and curves evoke the Indian image for the eagle, a native American symbol of power and prestige as well as the national symbol of the United States. The wings of the Shuttle suggest the wings of the eagle whose feathers, indicative of peace and friendship in Indian tradition, are captured by the U forms, a characteristic feature of Northwest coast Indian art. The nose of the Shuttle is the strong downward curve of the eagle's beak, and the Shuttle's forward windows, the eagle's eyes, represented through the tapered S forms again typical of this Indian art form. The basic black and red atoms orbiting the mission number recall the original NASA emblem while beneath, utilizing Indian ovoid forms, the major mission scientific experiment package LMS (Life and Materials Sciences) housed in the Shuttle's cargo bay is depicted in a manner reminiscent of totem-pole art. This image of a bird poised for flight, so common to Indian art, is counterpointed by an equally familiar Tsimshian Indian symbol, a pulsating sun with long hyperbolic rays, the symbol of life. Within each of these rays are now encased crystals, the products of this mission's 3 major, high-temperature materials processing furnaces. And as the sky in Indian lore is a lovely open country, home of the Sun Chief and accessible to travelers through a hole in the western horizon, so too, space is a vast and beckoning landscape for explorers launched beyond the horizon. Beneath the Tsimshian sun, the colors of the earth limb are appropriately enclosed by a red border representing life to the Northwest coast Indians. The Indian colors of red, navy blue, white, and black pervade the STS-78 path. To the right of the Shuttle-eagle, the constellation

  20. Positioning the Field: STS Futures (INTRODUCTION

    Directory of Open Access Journals (Sweden)

    Steven Epstein

    2016-07-01

    Full Text Available The thematic collection entitled “Positioning the Field: STS Futures” consists of seven short articles that had their origins in a conference organized by the Science, Knowledge, and Technology Section of the American Sociological Association. These position papers blend autobiography, history, and STS analysis to advocate for specific future directions in STS scholarship.

  1. Histone H3 Acetyl K9 and Histone H3 Tri Methyl K4 as Prognostic Markers for Patients with Cervical Cancer

    Directory of Open Access Journals (Sweden)

    Susanne Beyer

    2017-02-01

    Full Text Available Chromatin remodeling alters gene expression in carcinoma tissue. Although cervical cancer is the fourth most common cancer in women worldwide, a systematic study about the prognostic value of specific changes in the chromatin structure, such as histone acetylation or histone methylation, is missing. In this study, the expression of histone H3 acetyl K9, which is known to denote active regions at enhancers and promoters, and histone H3 tri methyl K4, which preferentially identifies active gene promoters, were examined as both show high metastatic potential. A panel of patients with cervical cancer was selected and the importance of the histone modifications concerning survival-time (overall survival and relapse-free survival was analyzed in 250 cases. Histone H3 acetyl K9 staining was correlated with low grading, low FIGO (TNM classification and the International Federation of Gynecology and Obstetrics status, negative N-status and low T-status in cervical cancer, showing a higher expression in adenocarcinoma than in squamous cell carcinoma. Cytoplasmic expression of histone H3 tri methyl K4 in a cervical cancer specimen was correlated with advanced T-status and poor prognosis. While cytoplasmic H3K4me3 expression seemed to be a marker of relapse-free survival, nuclear expression showed a correlation to poor prognosis in overall survival. Within this study, we analyzed the chemical modification of two histone proteins that are connected to active gene expression. Histone H3 acetyl K9 was found to be an independent marker of overall survival. Histone H3 tri methyl K4 was correlated with poor prognosis and it was found to be an independent marker of relapse-free survival. Therefore, we could show that chromatin remodeling plays an important role in cervical cancer biology.

  2. Histone H3 Acetyl K9 and Histone H3 Tri Methyl K4 as Prognostic Markers for Patients with Cervical Cancer.

    Science.gov (United States)

    Beyer, Susanne; Zhu, Junyan; Mayr, Doris; Kuhn, Christina; Schulze, Sandra; Hofmann, Simone; Dannecker, Christian; Jeschke, Udo; Kost, Bernd P

    2017-02-23

    Chromatin remodeling alters gene expression in carcinoma tissue. Although cervical cancer is the fourth most common cancer in women worldwide, a systematic study about the prognostic value of specific changes in the chromatin structure, such as histone acetylation or histone methylation, is missing. In this study, the expression of histone H3 acetyl K9, which is known to denote active regions at enhancers and promoters, and histone H3 tri methyl K4, which preferentially identifies active gene promoters, were examined as both show high metastatic potential. A panel of patients with cervical cancer was selected and the importance of the histone modifications concerning survival-time (overall survival and relapse-free survival) was analyzed in 250 cases. Histone H3 acetyl K9 staining was correlated with low grading, low FIGO (TNM classification and the International Federation of Gynecology and Obstetrics) status, negative N-status and low T-status in cervical cancer, showing a higher expression in adenocarcinoma than in squamous cell carcinoma. Cytoplasmic expression of histone H3 tri methyl K4 in a cervical cancer specimen was correlated with advanced T-status and poor prognosis. While cytoplasmic H3K4me3 expression seemed to be a marker of relapse-free survival, nuclear expression showed a correlation to poor prognosis in overall survival. Within this study, we analyzed the chemical modification of two histone proteins that are connected to active gene expression. Histone H3 acetyl K9 was found to be an independent marker of overall survival. Histone H3 tri methyl K4 was correlated with poor prognosis and it was found to be an independent marker of relapse-free survival. Therefore, we could show that chromatin remodeling plays an important role in cervical cancer biology.

  3. Relationship between gene body DNA methylation and intragenic H3K9me3 and H3K36me3 chromatin marks.

    Directory of Open Access Journals (Sweden)

    Maria A Hahn

    Full Text Available To elucidate the relationship between intragenic DNA methylation and chromatin marks, we performed epigenetic profiling of chromosome 19 in human bronchial epithelial cells (HBEC and in the colorectal cancer cell line HCT116 as well as its counterpart with double knockout of DNMT1 and DNMT3B (HCT116-DKO. Analysis of H3K36me3 profiles indicated that this intragenic mark of active genes is associated with two categories of genes: (i genes with low CpG density and H3K9me3 in the gene body or (ii genes with high CpG density and DNA methylation in the gene body. We observed that a combination of low CpG density in gene bodies together with H3K9me3 and H3K36me3 occupancy is a specific epigenetic feature of zinc finger (ZNF genes, which comprise 90% of all genes carrying both histone marks on chromosome 19. For genes with high intragenic CpG density, transcription and H3K36me3 occupancy were not changed in conditions of partial or intensive loss of DNA methylation in gene bodies. siRNA knockdown of SETD2, the major histone methyltransferase responsible for production of H3K36me3, did not reduce DNA methylation in gene bodies. Our study suggests that the H3K36me3 and DNA methylation marks in gene bodies are established largely independently of each other and points to similar functional roles of intragenic DNA methylation and intragenic H3K9me3 for CpG-rich and CpG-poor genes, respectively.

  4. STS and Researcher Intervention Strategies

    Directory of Open Access Journals (Sweden)

    Brian Martin

    2016-06-01

    Full Text Available When I learned about a concerted campaign against Australian vaccination critics, I decided to intervene in the debate. As a result, some proponents of vaccination turned on me, making abusive comments and complaining to university officials. At several points in this experience, I had to make choices about how to intervene or respond. STS perspectives offered valuable insights for understanding the dynamics of the controversy but provided little guidance for making decisions. Some reasons are offered for why STS lacks tools for guiding practical action in such situations.

  5. STS Interventions: Preparing, Defending, Learning

    Directory of Open Access Journals (Sweden)

    Brian Martin

    2016-06-01

    Full Text Available Intervening in the Australian vaccination debate, I found that STS perspectives helped me understand the controversy but gave little guidance on how to defend against attacks. Max Liboiron comments that preparation, including support networks, should precede interventions. I was well supported in my involvement in the vaccination debate, but those who openly advocate a stigmatized view may have difficulty gaining support. Teun Zuiderent-Jerak asks what is being learned when interventions reinforce existing categories. Controversy participants can learn by being exposed to STS perspectives; controversy researchers, when attacked, can gain new information and develop new research ideas.

  6. 基于MapGIS K9的基本农田监管系统的建设研究

    Institute of Scientific and Technical Information of China (English)

    覃艳琼; 覃豪杰

    2013-01-01

    基本农田数据库建设是保护耕地、实现基本农田规范亿管理的重要举措.本文以MapGIS K9地理信息套件为基础框架,介绍基本农田监管系统搭建方案,实现基本农田信息化动态管理,以全面提升基本农田保护水平.

  7. Holocarboxylase synthetase is a chromatin protein and interacts directly with histone H3 to mediate biotinylation of K9 and K18.

    Science.gov (United States)

    Bao, Baolong; Pestinger, Valerie; Hassan, Yousef I; Borgstahl, Gloria E O; Kolar, Carol; Zempleni, Janos

    2011-05-01

    Holocarboxylase synthetase (HCS) mediates the binding of biotin to lysine (K) residues in histones H2A, H3 and H4; HCS knockdown disturbs gene regulation and decreases stress resistance and lifespan in eukaryotes. We tested the hypothesis that HCS interacts physically with histone H3 for subsequent biotinylation. Co-immunoprecipitation experiments were conducted and provided evidence that HCS co-localizes with histone H3 in human cells; physical interactions between HCS and H3 were confirmed using limited proteolysis assays. Yeast two-hybrid (Y2H) studies revealed that the N-terminal and C-terminal domains in HCS participate in H3 binding. Recombinant human HCS was produced and exhibited biological activity, as evidenced by biotinylation of its known substrate, recombinant p67. Recombinant histone H3.2 and synthetic H3-based peptides were also good targets for biotinylation by recombinant HCS (rHCS) in vitro, based on tracing histone-bound biotin with [(3)H]biotin, streptavidin and anti-biotin antibody. Biotinylation site-specific antibodies were generated and revealed that both K9 and K18 in H3 were biotinylated by HCS. Collectively, these studies provide conclusive evidence that HCS interacts directly with histone H3, causing biotinylation of K9 and K18. We speculate that the targeting of HCS to distinct regions in human chromatin is mediated by DNA sequence, biotin, RNA, epigenetic marks or chromatin proteins.

  8. Signalling couples hair follicle stem cell quiescence with reduced histone H3 K4/K9/K27me3 for proper tissue homeostasis.

    Science.gov (United States)

    Lee, Jayhun; Kang, Sangjo; Lilja, Karin C; Colletier, Keegan J; Scheitz, Cornelia Johanna Franziska; Zhang, Ying V; Tumbar, Tudorita

    2016-04-15

    Mechanisms of plasticity to acquire different cell fates are critical for adult stem cell (SC) potential, yet are poorly understood. Reduced global histone methylation is an epigenetic state known to mediate plasticity in cultured embryonic SCs and T-cell progenitors. Here we find histone H3 K4/K9/K27me3 levels actively reduced in adult mouse skin and hair follicle stem cells (HFSCs) during G0 quiescence. The level of marks over specific gene promoters did not correlate to mRNA level changes in quiescent HFSCs. Skin hypomethylation during quiescence was necessary for subsequent progression of hair homeostasis (cycle). Inhibiting BMP signal, a known HFSC anti-proliferative factor, elevated HFSC methylation in vivo during quiescence prior to proliferation onset. Furthermore, removal of proliferation factors and addition of BMP4 reduced histone methylases and increased demethylases mRNAs in cultured skin epithelial cells. We conclude that signalling couples hair follicle stem cell quiescence with reduced H3 K4/K9/K27me3 levels for proper tissue homeostasis.

  9. STS-54 Physics of Toys

    Science.gov (United States)

    1993-01-01

    Greg Vogt, NASA Headquarters Education Specialist, and Carolyn Sumners, Houston Museum of Natural Science, give an overview of the spaceborne experiments that will take place on the STS-54 Endeavour mission. Mr. Vogt discusses the objectives and procedures of the experiments, which are structured around using toys to show the effects of microgravity. Mr. Vogt and Ms. Sumners then answer questions from the press.

  10. Game Design as STS Research

    Directory of Open Access Journals (Sweden)

    Joseph Dumit

    2017-09-01

    Full Text Available Game design offers a powerful pedagogical paradigm for engaging students in thinking and researching sociotechnical systems. Using the example of designing a game around fracking, this paper describes how game design grapples with emergent dynamic processes, and how students are drawn into becoming STS researchers.

  11. Stress relaxation damage in K9 glass plate irradiated by 1.06μm CW laser%1.06μm连续激光照射下K9玻璃板的应力松驰破坏

    Institute of Scientific and Technical Information of China (English)

    罗福; 孙承纬; 杜祥琬

    2001-01-01

    Based on the stress relaxation model in 1D planar geometry andthe visco-elastic constitutive equation, the temperature and stress histories in the K9 glass samples irradiated by CW laser beams (λ=1.06μm) have been calculated. The results indicate that the residual tensile stress due to the stress relaxation effect during cooling after the laser radiation may be greater than the tensile fracture strength of samples, while the maximum compression stress during the laser heating is less than the requirement for compression damage. For a K9 glass window of 3mm thickness, its damage due to the stress relaxation may be induced by a laser radiation of 0.946MW/cm2 for 0.2s. Therefore, the stress relaxation should be regarded as the main mechanism of damage in K9 glass windows while a CW laser beam (λ=1.06μm) irradiates it with large spot.%基于一维应力松弛模型和粘弹性本构关系,对1.06μm连续激光照射下K9玻璃板中的温升和应力作了计算,结果表明,由于应力松弛效应,激光停照后的冷却过程中产生的残余拉伸应力可达到玻璃的拉伸断裂强度阈值,而激光加热期间的最大压缩应力却小于压缩断裂强度要求。对3mm厚的K9玻璃板,由应力松弛引起破坏所要求的激光参数为946kWcm2作用0.2s,因而应力松弛可能为1.06μm连续激光大光斑照射下K9玻璃窗口损伤的主要模式。

  12. K9A1 METAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — METAR is a routine scheduled observation and is the primary observation code used in the United States to satisfy requirements for reporting surface meteorological...

  13. K9D7 METAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — METAR is a routine scheduled observation and is the primary observation code used in the United States to satisfy requirements for reporting surface meteorological...

  14. K9L2 METAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — METAR is a routine scheduled observation and is the primary observation code used in the United States to satisfy requirements for reporting surface meteorological...

  15. State Estimation for K9

    Science.gov (United States)

    Xu, Ru-Gang; Koga, Dennis (Technical Monitor)

    2001-01-01

    The goal of 'Estimate' is to take advantage of attitude information to produce better pose while staying flexible and robust. Currently there are several instruments that are used for attitude: gyros, inclinometers, and compasses. However, precise and useful attitude information cannot come from one instrument. Integration of rotational rates, from gyro data for example, would result in drift. Therefore, although gyros are accurate in the short-term, accuracy in the long term is unlikely. Using absolute instruments such as compasses and inclinometers can result in an accurate measurement of attitude in the long term. However, in the short term, the physical nature of compasses and inclinometers, and the dynamic nature of a mobile platform result in highly volatile and therefore useless data. The solution then is to use both absolute and relative data. Kalman Filtering is known to be able to combine gyro and compass/inclinometer data to produce stable and accurate attitude information. Since the model of motion is linear and the data comes in as discrete samples, a Discrete Kalman Filter was selected as the core of the new estimator. Therefore, 'Estimate' can be divided into two parts: the Discrete Kalman Filter and the code framework.

  16. K9S2 METAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — METAR is a routine scheduled observation and is the primary observation code used in the United States to satisfy requirements for reporting surface meteorological...

  17. K9V6 METAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — METAR is a routine scheduled observation and is the primary observation code used in the United States to satisfy requirements for reporting surface meteorological...

  18. HYPERFINE STRUCTURE SPECTRA FOR 4f45d6K\\-9/2→(23229.991)o\\-9/2 TRANSITION IN 143,145Nd+%143,145Nd+ 4f45d6K\\-9/2→(23229.991)o\\-9/2 跃迁超精细结构光谱

    Institute of Scientific and Technical Information of China (English)

    马洪良; 陆福全

    2001-01-01

    利用共线快离子束-激光光谱学方法测量了143,145Nd+ 6K\\-9/2→(23229.991)o\\-9/2跃迁的超精细结构光谱,得到了上、下能级的超精细相互作用常数.%Hyperfine structure spectra for 4f45d 6K\\-9/2→(23229.991)o\\-9/2 transition in 143,145Nd+ were measured by using collinear fast-ion-beam laser spectroscopy, and the magnetic dipole and electric quadrupole coupling constants of the metastable levels and excited levels were determined.

  19. MicroRNA-148b enhances proliferation and apoptosis in human renal cancer cells via directly targeting MAP3K9.

    Science.gov (United States)

    Nie, Fang; Liu, Tianming; Zhong, Liang; Yang, Xianggui; Liu, Yunhong; Xia, Hongwei; Liu, Xiaoqiang; Wang, Xiaoyan; Liu, Zhicheng; Zhou, Li; Mao, Zhaomin; Zhou, Qin; Chen, Tingmei

    2016-01-01

    Increasing evidence revealed that miRNAs, the vital regulators of gene expression, are involved in various cellular processes, including cell growth, differentiation, apoptosis and progression. In addition, miRNAs act as oncogenes and/or tumor suppressors. The present study aimed to verify the potential roles of miR148b in human renal cancer cells. miR‑148b was found to be downregulated in human renal cancel tissues and human renal cancer cell lines. Functional studies demonstrated that plasmid‑mediated overexpression of miR‑148b promoted cell proliferation, increased the S‑phase population of the cell cycle and enhanced apoptosis in the 786‑O and OS‑RC‑2 renal cancer cell lines, while it did not appear to affect the total number of viable cells according to a Cell Counting Kit‑8 assay. Subsequently, a luciferase reporter assay verified that miR148b directly targeted mitogen‑activated protein kinase (MAPK) kinase kinase 9 (MAP3K9), an upstream activator of MAPK kinase/c‑Jun N‑terminal kinase (JNK) signaling, suppressing the protein but not the mRNA levels. Furthermore, western blot analysis indicated that overexpression of miR148b in renal cancer cells inhibited MAPK/JNK signaling by decreasing the expression of phosphorylated (p)JNK. In addition, overexpression of MAP3K9 and pJNK was detected in clinical renal cell carcinoma specimens compared with that in their normal adjacent tissues. The present study therefore suggested that miR‑148b exerts an oncogenic function by enhancing the proliferation and apoptosis of renal cancer cells by inhibiting the MAPK/JNK pathway.

  20. Chromosomal distribution of H3K4me2, H3K9me2 and 5-methylcytosine: variations associated with polyploidy and hybridization in Brachiaria (Poaceae).

    Science.gov (United States)

    de Paula, Cristina Maria Pinto; Souza Sobrinho, Fausto; Techio, Vânia Helena

    2016-06-01

    Assessment of chromosomal distribution of modified histones and 5-methylcytosine shown that there are diversification of chromosomal types among species of Brachiaria and its interspecific hybrids. Histone post-translational modifications and DNA methylation are epigenetic processes that are involved in structural and functional organization of the genome. This study compared the chromosomal distribution of modified histones and 5-methylcytosine (5-mCyt) in species and interspecific hybrids of Brachiaria with different ploidy levels and reproduction modes. The relation between H3K9me2 and 5-mCyt was observed in the nucleolus organizer region, centromeric central domain and pericentromeric region. H3K4me2 was detected in euchromatic domains, mainly in the terminal chromosomal regions. Comparison of chromosomal distribution among species and hybrids showed greater variation of chromosomal types for the H3K9me2 in B. decumbens (tetraploid and apomictic species) and the 963 hybrid, while, for the H3K4me2, the variation was higher in B. brizantha and B. decumbens (tetraploid and apomictic species) and 963 hybrid. The chromosome distribution of 5-mCyt was similar between B. brizantha and B. decumbens, which differ from the distribution observed in B. ruziziensis (diploid and sexual species). Significant alterations in DNA methylation were observed in the artificially tetraploidized B. ruziziensis and in the interspecific hybrids, possibly as result of hybridization and polyploidization processes. The monitoring of histone modifications and DNA methylation allowed categorizing nuclear and chromosomal distribution of these epigenetic marks, thus contributing to the knowledge of composition and structure of the genome/epigenome of Brachiaria species and hybrids. These data can be useful for speciation and genome evolution studies in genus Brachiaria, and represent important markers to explore relationships between genomes.

  1. Central role of Drosophila SU(VAR)3-9 in histone H3-K9 methylation and heterochromatic gene silencing.

    Science.gov (United States)

    Schotta, Gunnar; Ebert, Anja; Krauss, Veiko; Fischer, Andreas; Hoffmann, Jan; Rea, Stephen; Jenuwein, Thomas; Dorn, Rainer; Reuter, Gunter

    2002-03-01

    Su(var)3-9 is a dominant modifier of heterochromatin-induced gene silencing. Like its mammalian and Schizosaccharomyces pombe homologues, Su(var) 3-9 encodes a histone methyltransferase (HMTase), which selectively methylates histone H3 at lysine 9 (H3-K9). In Su(var)3-9 null mutants, H3-K9 methylation at chromocentre heterochromatin is strongly reduced, indicating that SU(VAR)3-9 is the major heterochromatin-specific HMTase in Drosophila. SU (VAR)3-9 interacts with the heterochromatin-associated HP1 protein and with another silencing factor, SU(VAR)3-7. Notably, SU(VAR)3-9-HP1 interaction is interdependent and governs distinct localization patterns of both proteins. In Su(var)3-9 null mutants, concentration of HP1 at the chromocentre is nearly lost without affecting HP1 accumulation at the fourth chromosome. By contrast, in HP1 null mutants SU(VAR)3-9 is no longer restricted at heterochromatin but broadly dispersed across the chromosomes. Despite this interdependence, Su(var)3-9 dominates the PEV modifier effects of HP1 and Su(var)3-7 and is also epistatic to the Y chromosome effect on PEV. Finally, the human SUV39H1 gene is able to partially rescue Su(var)3-9 silencing defects. Together, these data indicate a central role for the SU(VAR)3-9 HMTase in heterochromatin-induced gene silencing in Drosophila.

  2. Encountering social work through STS

    DEFF Research Database (Denmark)

    Birk, Rasmus

    Encountering social work through STS: Marginalization, materials and knowledge In this presentation, I attempt to produce an encounter between STS and social work. Concretely, I focus on the subset of social work called “local community work”, which in Denmark is used to intervene on marginalized...... and their inhabitants. Local community work derives from this assemblage of policy and knowledge as the “social” intervention commonly deployed. Based on an ethnographic field work, I examine how local community practices attempt to interpellate specific futures for individuals and their local environments. I do...... this by examining the materials and types of knowledge that participate in shaping local community work practices and encounters between local community workers and residents in marginalized housing areas. Through this analysis, I argue that social work research can benefit from orienting itself more concretely...

  3. STS-53 Launch and Landing

    Science.gov (United States)

    1992-01-01

    Footage of various stages of the STS-53 Discovery launch is shown, including shots of the crew at breakfast, getting suited up, and departing to board the Orbiter. The launch is seen from many vantage points, as is the landing. On-orbit activities show the crew performing several medical experiments, such as taking a picture of the retina and measuring the pressure on the eyeball. One crewmember demonstrates how to use the rowing machine in an antigravity environment.

  4. Effect of sleep deprivation on H3K9 and H3K4 methylation in the different brain region of rats%REM期睡眠剥夺对大鼠不同脑区组蛋白H3K9和H3K4甲基化水平的影响

    Institute of Scientific and Technical Information of China (English)

    马安东; 马妮; 史明; 田丰仓; 吴中亮

    2016-01-01

    目的:研究REM期睡眠剥夺对成年大鼠不同脑区包括海马、腹内侧前额叶皮质、下丘脑和中缝核群的组蛋白H3K9和H3K4三甲基化水平的影响.方法:将成年SD大鼠随机分为正常对照组(con)、睡眠剥夺1d组(SD1 d)、睡眠剥夺3d组(SD3 d)、睡眠剥夺6d组(SD6 d).采用改良式多平台水环境法建立REM期睡眠剥夺模型,分别于睡眠剥夺后1、3、6d断头取脑,用免疫荧光染色与Western Blot检测大鼠海马、腹内侧前额叶皮质、下丘脑和中缝核群H3K9和H3K4三甲基化水平的变化,最终结果进行统计学分析.结果:Western Blot结果显示:(1)海马、腹内侧前额叶皮质三个SD组H3K9三甲基化水平均低于control组(P<0.05),H3K4三甲基化水平均高于control组(P<0.05);(2)下丘脑三个SD组H3K9三甲基化水平均低于control组(P<0.01),但SD1 d组H3K4三甲基化水平高于control组(P<0.05),SD3 d、SD6 d组H3K4三甲基化水平低于control组(P<0.05);(3)中缝核群三个SD组H3K9和H3K4三甲基化水平均高于control组(P<0.05).对海马与下丘脑免疫荧光验证H3K9三甲基化结果与Western Blot结果趋势一致,且说明细胞无减少.结论:睡眠剥夺可能与海马、腹内侧前额叶皮质、下丘脑和中缝核群的组蛋白H3K9和H3K4三甲基化水平密切相关.

  5. STS-69 Clears the tower

    Science.gov (United States)

    1995-01-01

    A pack of astronauts that run under the banner Dog Crew II heads for the stars. Liftoff of the Space Shuttle Endeavour from Launch Pad 39A occurred at 11:09:00.052 a.m. EDT, Sept. 7, 1995. 'Every dog has its day and today is your day,' KSC Orbiter Test Conductor Roger Gillette pledged to STS-69 Mission Commander David M. Walker, Pilot Kenneth D. Cockrell, Payload Commander James S. Voss and Mission Specialists Michael L. Gernhardt and James H. Newman prior to launch. The STS-69 astronaut crew developed a strong sense of comaraderie as they went through their flight training, and dubbed themselves the Dog Crew II to carry on a tradition that arose during an earlier Shuttle flight -- STS-53 -- to which both Voss and Walker were assigned. Each crew member adopted a dog-theme name: Walker is Red Dog; Cockrell, Cujo; Voss, Dogface; Newman, Pluto; and Gernhardt, the only space rookie, Underdog. A special patch, featuring a bulldog in a doghouse shaped like the Space Shuttle, was designed for the astronauts and other flight team members to wear. The Dog Crew II is embarking on an 11-day multifaceted mission featuring two free-flying scientific research spacecraft as well as a host of experiments in both the payload bay and the middeck. Also scheduled is an extravehicular activity, or spacewalk.

  6. Inhibition of H3K9me2 Reduces Hair Cell Regeneration after Hair Cell Loss in the Zebrafish Lateral Line by Down-Regulating the Wnt and Fgf Signaling Pathways

    Directory of Open Access Journals (Sweden)

    Dongmei eTang

    2016-05-01

    Full Text Available The activation of neuromast supporting cell (SC proliferation leads to hair cell (HC regeneration in the zebrafish lateral line. Epigenetic mechanisms have been reported that regulate HC regeneration in the zebrafish lateral line, but the role of H3K9me2 in HC regeneration after HC loss remains poorly understood. In this study, we focused on the role of H3K9me2 in HC regeneration following neomycin-induced HC loss. To investigate the effects of H3K9me2 in HC regeneration, we took advantage of the G9a/GLP-specific inhibitor BIX01294 that significantly reduces the dimethylation of H3K9. We found that BIX01294 significantly reduced HC regeneration after neomycin-induced HC loss in the zebrafish lateral line. BIX01294 also significantly reduced the proliferation of neuromast cells and led to fewer SCs in the lateral line. In situ hybridization showed that BIX01294 significantly down-regulated the Wnt and Fgf signaling pathways, which resulted in reduced SC proliferation and HC regeneration in the neuromasts of the lateral line. Altogether, our results suggest that down-regulation of H3K9me2 significantly decreases HC regeneration after neomycin-induced HC loss through inactivation of the Wnt/β-catenin and Fgf signaling pathways. Thus H3K9me2 plays a critical role in HC regeneration.

  7. Inhibition of H3K9me2 Reduces Hair Cell Regeneration after Hair Cell Loss in the Zebrafish Lateral Line by Down-Regulating the Wnt and Fgf Signaling Pathways

    Science.gov (United States)

    Tang, Dongmei; Lin, Qin; He, Yingzi; Chai, Renjie; Li, Huawei

    2016-01-01

    The activation of neuromast (NM) supporting cell (SC) proliferation leads to hair cell (HC) regeneration in the zebrafish lateral line. Epigenetic mechanisms have been reported that regulate HC regeneration in the zebrafish lateral line, but the role of H3K9me2 in HC regeneration after HC loss remains poorly understood. In this study, we focused on the role of H3K9me2 in HC regeneration following neomycin-induced HC loss. To investigate the effects of H3K9me2 in HC regeneration, we took advantage of the G9a/GLP-specific inhibitor BIX01294 that significantly reduces the dimethylation of H3K9. We found that BIX01294 significantly reduced HC regeneration after neomycin-induced HC loss in the zebrafish lateral line. BIX01294 also significantly reduced the proliferation of NM cells and led to fewer SCs in the lateral line. In situ hybridization showed that BIX01294 significantly down-regulated the Wnt and Fgf signaling pathways, which resulted in reduced SC proliferation and HC regeneration in the NMs of the lateral line. Altogether, our results suggest that down-regulation of H3K9me2 significantly decreases HC regeneration after neomycin-induced HC loss through inactivation of the Wnt/β-catenin and Fgf signaling pathways. Thus H3K9me2 plays a critical role in HC regeneration. PMID:27303264

  8. Alcohol-induced histone H3K9 hyperacetylation and cardiac hypertrophy are reversed by a histone acetylases inhibitor anacardic acid in developing murine hearts.

    Science.gov (United States)

    Peng, Chang; Zhang, Weihua; Zhao, Weian; Zhu, Jing; Huang, Xupei; Tian, Jie

    2015-06-01

    The expression of cardiac genes is precisely regulated, and any perturbation may cause developmental defects. In a previous study, we demonstrated that alcohol consumption during pregnancy could lead to uncontrolled expressions of cardiac genes and eventually result in cardiac dysplasia. However, the underlying mechanisms remain unclear. In the present study, we have investigated the alcohol-induced cardiac hypertrophy and its potential mechanisms. Furthermore, the protective effect of anacardic acid against the alcohol-induced cardiac hypertrophy has been explored in experimental mice. C57BL/6 pregnant mice were gavaged with 56% ethanol or saline and the hearts of their fetus were collected for analysis. Binding of p300, CBP, PCAF, SRC1, except GCN5, were increased to the NKX2.5 promoter in fetal mouse hearts exposed to alcohol. Increased acetylation of H3K9 and increased mRNA expression of NKX2.5, β-MHC and Cx43 were observed in the same samples. Treatment with a pan-acetylase inhibitor, anacardic acid, reduced the binding affinity of p300 and PCAF to the NKX2.5, β-MHC, Cx43 promoters and attenuated H3K9 hyperacetylation. Interestingly, anacardic acid down-regulated over-expression of these cardiac genes induced by alcohol and ultimately attenuated ethanol-induced cardiac hypertrophy in fetal mice. Our results indicate that alcohol exposure during pregnancy could lead to fetal cardiac hypertrophy. The over-expression of NKX2.5, β-MHC, Cx43 mediated by p300 and PCAF may be critical mechanisms of alcohol-induced cardiac hypertrophy. Anacardic acid can down-regulate the over-expression of cardiac genes and reverse cardiac hypertrophy caused by alcohol treatment in pregnant mice, suggesting it could be a potential therapeutic agent for the treatment of cardiac hypertrophy. Copyright © 2015 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.

  9. MeCP2 binds to nucleosome free (linker DNA) regions and to H3K9/H3K27 methylated nucleosomes in the brain

    Science.gov (United States)

    Thambirajah, Anita A.; Ng, Marlee K.; Frehlick, Lindsay J.; Li, Andra; Serpa, Jason J.; Petrotchenko, Evgeniy V.; Silva-Moreno, Begonia; Missiaen, Kristal K.; Borchers, Christoph H.; Adam Hall, J.; Mackie, Ryan; Lutz, Frank; Gowen, Brent E.; Hendzel, Michael; Georgel, Philippe T.; Ausió, Juan

    2012-01-01

    Methyl-CpG-binding protein 2 (MeCP2) is a chromatin-binding protein that mediates transcriptional regulation, and is highly abundant in brain. The nature of its binding to reconstituted templates has been well characterized in vitro. However, its interactions with native chromatin are less understood. Here we show that MeCP2 displays a distinct distribution within fractionated chromatin from various tissues and cell types. Artificially induced global changes in DNA methylation by 3-aminobenzamide or 5-aza-2′-deoxycytidine, do not significantly affect the distribution or amount of MeCP2 in HeLa S3 or 3T3 cells. Most MeCP2 in brain is chromatin-bound and localized within highly nuclease-accessible regions. We also show that, while in most tissues and cell lines, MeCP2 forms stable complexes with nucleosome, in brain, a fraction of it is loosely bound to chromatin, likely to nucleosome-depleted regions. Finally, we provide evidence for novel associations of MeCP2 with mononucleosomes containing histone H2A.X, H3K9me2 and H3K27me3 in different chromatin fractions from brain cortex and in vitro. We postulate that the functional compartmentalization and tissue-specific distribution of MeCP2 within different chromatin types may be directed by its association with nucleosomes containing specific histone variants, and post-translational modifications. PMID:22144686

  10. Targeted genetic dependency screen facilitates identification of actionable mutations in FGFR4, MAP3K9, and PAK5 in lung cancer.

    Science.gov (United States)

    Fawdar, Shameem; Trotter, Eleanor W; Li, Yaoyong; Stephenson, Natalie L; Hanke, Franziska; Marusiak, Anna A; Edwards, Zoe C; Ientile, Sara; Waszkowycz, Bohdan; Miller, Crispin J; Brognard, John

    2013-07-23

    Approximately 70% of patients with non-small-cell lung cancer present with late-stage disease and have limited treatment options, so there is a pressing need to develop efficacious targeted therapies for these patients. This remains a major challenge as the underlying genetic causes of ~50% of non-small-cell lung cancers remain unknown. Here we demonstrate that a targeted genetic dependency screen is an efficient approach to identify somatic cancer alterations that are functionally important. By using this approach, we have identified three kinases with gain-of-function mutations in lung cancer, namely FGFR4, MAP3K9, and PAK5. Mutations in these kinases are activating toward the ERK pathway, and targeted depletion of the mutated kinases inhibits proliferation, suppresses constitutive activation of downstream signaling pathways, and results in specific killing of the lung cancer cells. Genomic profiling of patients with lung cancer is ushering in an era of personalized medicine; however, lack of actionable mutations presents a significant hurdle. Our study indicates that targeted genetic dependency screens will be an effective strategy to elucidate somatic variants that are essential for lung cancer cell viability.

  11. GATA-1 Inhibits PU.1 Gene via DNA and Histone H3K9 Methylation of Its Distal Enhancer in Erythroleukemia.

    Directory of Open Access Journals (Sweden)

    Pavel Burda

    Full Text Available GATA-1 and PU.1 are two important hematopoietic transcription factors that mutually inhibit each other in progenitor cells to guide entrance into the erythroid or myeloid lineage, respectively. PU.1 controls its own expression during myelopoiesis by binding to the distal URE enhancer, whose deletion leads to acute myeloid leukemia (AML. We herein present evidence that GATA-1 binds to the PU.1 gene and inhibits its expression in human AML-erythroleukemias (EL. Furthermore, GATA-1 together with DNA methyl Transferase I (DNMT1 mediate repression of the PU.1 gene through the URE. Repression of the PU.1 gene involves both DNA methylation at the URE and its histone H3 lysine-K9 methylation and deacetylation as well as the H3K27 methylation at additional DNA elements and the promoter. The GATA-1-mediated inhibition of PU.1 gene transcription in human AML-EL mediated through the URE represents important mechanism that contributes to PU.1 downregulation and leukemogenesis that is sensitive to DNA demethylation therapy.

  12. GATA-1 Inhibits PU.1 Gene via DNA and Histone H3K9 Methylation of Its Distal Enhancer in Erythroleukemia.

    Science.gov (United States)

    Burda, Pavel; Vargova, Jarmila; Curik, Nikola; Salek, Cyril; Papadopoulos, Giorgio Lucio; Strouboulis, John; Stopka, Tomas

    2016-01-01

    GATA-1 and PU.1 are two important hematopoietic transcription factors that mutually inhibit each other in progenitor cells to guide entrance into the erythroid or myeloid lineage, respectively. PU.1 controls its own expression during myelopoiesis by binding to the distal URE enhancer, whose deletion leads to acute myeloid leukemia (AML). We herein present evidence that GATA-1 binds to the PU.1 gene and inhibits its expression in human AML-erythroleukemias (EL). Furthermore, GATA-1 together with DNA methyl Transferase I (DNMT1) mediate repression of the PU.1 gene through the URE. Repression of the PU.1 gene involves both DNA methylation at the URE and its histone H3 lysine-K9 methylation and deacetylation as well as the H3K27 methylation at additional DNA elements and the promoter. The GATA-1-mediated inhibition of PU.1 gene transcription in human AML-EL mediated through the URE represents important mechanism that contributes to PU.1 downregulation and leukemogenesis that is sensitive to DNA demethylation therapy.

  13. piRNA pathway targets active LINE1 elements to establish the repressive H3K9me3 mark in germ cells.

    Science.gov (United States)

    Pezic, Dubravka; Manakov, Sergei A; Sachidanandam, Ravi; Aravin, Alexei A

    2014-07-01

    Transposable elements (TEs) occupy a large fraction of metazoan genomes and pose a constant threat to genomic integrity. This threat is particularly critical in germ cells, as changes in the genome that are induced by TEs will be transmitted to the next generation. Small noncoding piwi-interacting RNAs (piRNAs) recognize and silence a diverse set of TEs in germ cells. In mice, piRNA-guided transposon repression correlates with establishment of CpG DNA methylation on their sequences, yet the mechanism and the spectrum of genomic targets of piRNA silencing are unknown. Here we show that in addition to DNA methylation, the piRNA pathway is required to maintain a high level of the repressive H3K9me3 histone modification on long interspersed nuclear elements (LINEs) in germ cells. piRNA-dependent chromatin repression targets exclusively full-length elements of actively transposing LINE families, demonstrating the remarkable ability of the piRNA pathway to recognize active elements among the large number of genomic transposon fragments.

  14. STS-111 Crew Training Clip

    Science.gov (United States)

    2002-05-01

    The STS-111 Crew is in training for space flight. The crew consists of Commander Ken Cockrell, Pilot Paul Lockhart, Mission Specialists Franklin Chang-Diaz and Philippe Perrin. The crew training begins with Post Insertion Operations with the Full Fuselage Trainer (FFT). Franklin Chang-Diaz, Philippe Perrin and Paul Lockhart are shown in training for airlock and Neutral Buoyancy Lab (NBL) activities. Bailout in Crew Compartment Training (CCT) with Expedition Five is also shown. The crew also gets experience with photography, television, and habitation equipment.

  15. STS-109 Crew Interviews - Carey

    Science.gov (United States)

    2002-01-01

    STS-109 pilot Duane G. Carey is seen during a prelaunch interview. He answers questions about his inspiration to become an astronaut and his career path. He gives details on the mission's goals and significance, as well as an extended description of his role in the Orbiter's return landing. As its primary objective, this mission has the maintenance of the Hubble Space Telescope (HST). Following the Columbia Orbiter's rendezvous with the telescope, extravehicular activities (EVA) will focus on repairs to and augmentation of the HST.

  16. STS-109 Crew Interviews - Carey

    Science.gov (United States)

    2002-01-01

    STS-109 pilot Duane G. Carey is seen during a prelaunch interview. He answers questions about his inspiration to become an astronaut and his career path. He gives details on the mission's goals and significance, as well as an extended description of his role in the Orbiter's return landing. As its primary objective, this mission has the maintenance of the Hubble Space Telescope (HST). Following the Columbia Orbiter's rendezvous with the telescope, extravehicular activities (EVA) will focus on repairs to and augmentation of the HST.

  17. Loss of the DNA methyltransferase MET1 Induces H3K9 hypermethylation at PcG target genes and redistribution of H3K27 trimethylation to transposons in Arabidopsis thaliana.

    Directory of Open Access Journals (Sweden)

    Angelique Deleris

    Full Text Available Dimethylation of histone H3 lysine 9 (H3K9m2 and trimethylation of histone H3 lysine 27 (H3K27m3 are two hallmarks of transcriptional repression in many organisms. In Arabidopsis thaliana, H3K27m3 is targeted by Polycomb Group (PcG proteins and is associated with silent protein-coding genes, while H3K9m2 is correlated with DNA methylation and is associated with transposons and repetitive sequences. Recently, ectopic genic DNA methylation in the CHG context (where H is any base except G has been observed in globally DNA hypomethylated mutants such as met1, but neither the nature of the hypermethylated loci nor the biological significance of this epigenetic phenomenon have been investigated. Here, we generated high-resolution, genome-wide maps of both H3K9m2 and H3K27m3 in wild-type and met1 plants, which we integrated with transcriptional data, to explore the relationships between these two marks. We found that ectopic H3K9m2 observed in met1 can be due to defects in IBM1-mediated H3K9m2 demethylation at some sites, but most importantly targets H3K27m3-marked genes, suggesting an interplay between these two silencing marks. Furthermore, H3K9m2/DNA-hypermethylation at these PcG targets in met1 is coupled with a decrease in H3K27m3 marks, whereas CG/H3K9m2 hypomethylated transposons become ectopically H3K27m3 hypermethylated. Our results bear interesting similarities with cancer cells, which show global losses of DNA methylation but ectopic hypermethylation of genes previously marked by H3K27m3.

  18. Identification and characterization of the Spodoptera Su(var 3-9 histone H3K9 trimethyltransferase and its effect in AcMNPV infection.

    Directory of Open Access Journals (Sweden)

    Binbin Li

    Full Text Available Histone H3-lysine(9 (H3K9 trimethyltransferase gene Su(var 3-9 was cloned and identified in three Spodoptera insects, Spodopterafrugiperda (S. frugiperda, S. exigua and S. litura. Sequence analysis showed that Spodoptera Su(var 3-9 is highly conserved evolutionarily. Su(var 3-9 protein was found to be localized in the nucleus in Sf9 cells, and interact with histone H3, and the heterochromatin protein 1a (HP1a and HP1b. A dose-dependent enzymatic activity was found at both 27 °C and 37 °C in vitro, with higher activity at 27 °C. Addition of specific inhibitor chaetocin resulted in decreased histone methylation level and host chromatin relaxation. In contrast, overexpression of Su(var 3-9 caused increased histone methylation level and cellular genome compaction. In AcMNV-infected Sf9 cells, the transcription of Su(var 3-9 increased at late time of infection, although the mRNA levels of most cellular genes decreased. Pre-treatment of Sf9 cells with chaetocin speeded up viral DNA replication, and increased the transcription level of a variety of virus genes, whereas in Sf9 cells pre-transformed with Su(var 3-9 expression vector, viral DNA replication slow down slightly. These findings suggest that Su(var 3-9 might participate in the viral genes expression an genome replication repression during AcMNPV infection. It provided a new insight for the understanding virus-host interaction mechanism.

  19. SIM.T-K9.1: Canada/Peru bilateral comparison of standard platinum resistance thermometers from 0.01 °C to 419.527 °C

    Science.gov (United States)

    Hill, K. D.; Barrientos, A.; Guillen, E.

    2015-01-01

    The Consultative Committee for Thermometry has organized Key Comparison 9 (CCT-K9) as an updated replacement for CCT-K3. A comparison of the fixed-point temperature standards of Peru with those of Canada was carried out following a protocol that closely resembles CCT-K9. The comparison was limited to the fixed points of tin and zinc, with NRC the pilot laboratory and the eventual link to CCT-K9 (currently, CCT-K9 remains a work in progress). The comparison protocol required INDECOPI to select, characterize, and transport (i.e. hand carry) the SPRTs to NRC. Following the measurements at NRC, the SPRTs were returned and re-measured at INDECOPI to close the loop and assess any changes that occurred in the SPRTs over the course of the measurements. The following degrees of equivalence, D, and expanded uncertainties, U (k = 2), were obtained via this comparison. T / °C DINDECOPI / mK U / mK 419.527 1.58 4.6 231.928 -2.12 3.9 SIM.T-K9.1 has demonstrated the compatibility of the fixed points of INDECOPI with those maintained by NRC within the combined uncertainties, and therefore serves to verify the intended calibration and measurement capabilities envisioned for INDECOPI, Peru. The results can be linked to NMIs globally following CCT-K9, with NRC as the linking participant. Main text. To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCT, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

  20. The PR-Set7 binding domain of Riz1 is required for the H4K20me1-H3K9me1 trans-tail 'histone code' and Riz1 tumor suppressor function.

    Science.gov (United States)

    Congdon, Lauren M; Sims, Jennifer K; Tuzon, Creighton T; Rice, Judd C

    2014-04-01

    PR-Set7/Set8/KMT5a is the sole histone H4 lysine 20 monomethyltransferase (H4K20me1) in metazoans and is essential for proper cell division and genomic stability. We unexpectedly discovered that normal cellular levels of monomethylated histone H3 lysine 9 (H3K9me1) were also dependent on PR-Set7, but independent of its catalytic activity. This observation suggested that PR-Set7 interacts with an H3K9 monomethyltransferase to establish the previously reported H4K20me1-H3K9me1 trans-tail 'histone code'. Here we show that PR-Set7 specifically and directly binds the C-terminus of the Riz1/PRDM2/KMT8 tumor suppressor and demonstrate that the N-terminal PR/SET domain of Riz1 preferentially monomethylates H3K9. The PR-Set7 binding domain was required for Riz1 nuclear localization and maintenance of the H4K20me1-H3K9me1 trans-tail 'histone code'. Although Riz1 can function as a repressor, Riz1/H3K9me1 was dispensable for the repression of genes regulated by PR-Set7/H4K20me1. Frameshift mutations resulting in a truncated Riz1 incapable of binding PR-Set7 occur frequently in various aggressive cancers. In these cancer cells, expression of wild-type Riz1 restored tumor suppression by decreasing proliferation and increasing apoptosis. These phenotypes were not observed in cells expressing either the Riz1 PR/SET domain or PR-Set7 binding domain indicating that Riz1 methyltransferase activity and PR-Set7 binding domain are both essential for Riz1 tumor suppressor function.

  1. The PR-Set7 binding domain of Riz1 is required for the H4K20me1-H3K9me1 trans-tail ‘histone code’ and Riz1 tumor suppressor function

    Science.gov (United States)

    Congdon, Lauren M.; Sims, Jennifer K.; Tuzon, Creighton T.; Rice, Judd C.

    2014-01-01

    PR-Set7/Set8/KMT5a is the sole histone H4 lysine 20 monomethyltransferase (H4K20me1) in metazoans and is essential for proper cell division and genomic stability. We unexpectedly discovered that normal cellular levels of monomethylated histone H3 lysine 9 (H3K9me1) were also dependent on PR-Set7, but independent of its catalytic activity. This observation suggested that PR-Set7 interacts with an H3K9 monomethyltransferase to establish the previously reported H4K20me1-H3K9me1 trans-tail ‘histone code’. Here we show that PR-Set7 specifically and directly binds the C-terminus of the Riz1/PRDM2/KMT8 tumor suppressor and demonstrate that the N-terminal PR/SET domain of Riz1 preferentially monomethylates H3K9. The PR-Set7 binding domain was required for Riz1 nuclear localization and maintenance of the H4K20me1-H3K9me1 trans-tail ‘histone code’. Although Riz1 can function as a repressor, Riz1/H3K9me1 was dispensable for the repression of genes regulated by PR-Set7/H4K20me1. Frameshift mutations resulting in a truncated Riz1 incapable of binding PR-Set7 occur frequently in various aggressive cancers. In these cancer cells, expression of wild-type Riz1 restored tumor suppression by decreasing proliferation and increasing apoptosis. These phenotypes were not observed in cells expressing either the Riz1 PR/SET domain or PR-Set7 binding domain indicating that Riz1 methyltransferase activity and PR-Set7 binding domain are both essential for Riz1 tumor suppressor function. PMID:24423864

  2. 基于MapGIS K9的林业地图符号库构建及符号化实施%Construction of Symbol Database and Symboltzation Execution of Forestry Map Based on MapGIS K9

    Institute of Scientific and Technical Information of China (English)

    司瑶; 武红敢

    2011-01-01

    为提高林业地图的制图效率和智能化水平,笔者提出了基于MapGIS K9平台的林业地图符号化方案.首先按照国家林业局2010年10月颁布的构建林业地图符号库,然后建立符号配置规则库以实现数字环境下符号配置规则的表达,最后在Map-GIS Objects组件开发技术支持下实现了符号化模块,按照符号配置规则对林业专题数据进行可视化表达.实践证明该方案能够基本满足包括森林资源规划设计调查成果图在内的常见林业地图制图要求.%In order to improve the cartography efficiency and the intelligent level of foreatry map,we put lorward the symbolization scheme of forestry map based on MapGIS K9 plarform.Firstly,the symbol database of forestry map wad constructed according to《The Graphic Representation of Foreatry Map》which was issued by The National Forestry Bureau in October2010.Then,the symbol configuration rule database was established to realize the expression of symbol configuration rule in the digital environment.Finally,the symbolization module was realized based on MapGIS objects component development technology.Sccording to the symbol configuration fule,the rhematic data of foreatry were done the visualization.The practice proved that the scheme could basically satisfy the cartography requirements of common forestry maps which included the foreat management inventory map.

  3. STS-112 Crew Interviews: Ashby

    Science.gov (United States)

    2002-01-01

    STS-112 Mission Commander Jeffrey Ashby is seen during this preflight interview, answering questions about his inspiration in becoming an astronaut and his career path and provides an overview of the mission. Ashby outlines his role in the mission in general, and specifically during the docking and extravehicular activities (EVAs). He describes the payload (S1 truss) and the importance that the S1 truss will have in the development of the International Space Station (ISS). Ashby discusses the delivery and installation of the S1 truss scheduled to be done in the planned EVAs in some detail. He touches on the use and operation of the Canadarm 2 robotic arm in this process and outlines what supplies will be exchanged with the resident crew of the ISS during transfer activities. He ends with his thoughts on the value of the ISS in fostering international cooperation.

  4. Caring for Participation in STS

    DEFF Research Database (Denmark)

    Nielsen, Karen Dam

    2015-01-01

    In this short report from the EASST Conference 2014, I sketch a handful of presentations that engaged with ‘participation’. Two tracks, in particular, offered interesting analyses and conceptual experiments. The first track contained primarily empirically driven studies of ‘technologies of partic......In this short report from the EASST Conference 2014, I sketch a handful of presentations that engaged with ‘participation’. Two tracks, in particular, offered interesting analyses and conceptual experiments. The first track contained primarily empirically driven studies of ‘technologies...... of participation’ in (health) care and provided illustrations of the conceptual ambiguities and empirical implications that continue to make participation a problematic, yet highly relevant and intriguing STS-topic. The second track featured presentations that more explicitly contributed new analytical tools...

  5. STS-112 Crew Interviews - Magnus

    Science.gov (United States)

    2002-01-01

    STS-112 Mission Specialist 2 Sandra H. Magnus is seen during a prelaunch interview. She answers questions about her inspiration to become an astronaut and her career path. She gives details on the mission's goals, the most significant of which will be the installation of the S-1 truss structure on the International Space Station (ISS). The installation, one in a series of truss extending missions, will be complicated and will require the use of the robotic arm as well as extravehicular activity (EVA) by astronauts. Magnus also describes her function in the performance of transfer operations. Brief descriptions are given of experiments on board the ISS as well as on board the Shuttle.

  6. STS-112 Crew Interviews: Yurchikhin

    Science.gov (United States)

    2002-01-01

    A preflight interview with mission specialist Fyodor Yurchikhin is presented. He worked for a long time in Energia in the Russian Mission Control Center (MCC). Yurchikhin discusses the main goal of the STS-112 flight, which is to install the Integrated Truss Assembly S1 (Starboard Side Thermal Radiator Truss) on the International Space Station. He also talks about the three space walks required to install the S1. After the installation of S1, work with the bolts and cameras are performed. Yurchikhin is involved in working with nitrogen and ammonia jumpers. He expresses the complexity of his work, but says that he and the other crew members are ready for the challenge.

  7. A quick solution structure determination of the fully oxidized double mutant K9-10A cytochrome c7 from Desulfuromonas acetoxidans and mechanistic implications.

    Science.gov (United States)

    Assfalg, Michael; Bertini, Ivano; Turano, Paola; Bruschi, Mireille; Durand, Marie-Claire; Giudici-Orticoni, Marie-Thérèse; Dolla, Alain

    2002-02-01

    Lysines 9 and 10 in Desulfuromonas acetoxidans cytochrome c7, which could be involved in the interaction mechanism with the redox partners, have been replaced by alanine residues using site-directed mutagenesis. The solution structure of the fully oxidized form of K9-10A cytochrome c7, which is paramagnetic with three paramagnetic centers, has been determined via 1H NMR. The assignment of the spectra has been performed through an automatic program whose algorithm and strategy are here described. The assignment of the NOESY spectra has been further extended by back calculating the NOESY maps. The final number of meaningful NOE-based upper distance limits was 1186. In the Restrained Energy Minimization calculations, 147 pseudocontact shift constraints were also included, which showed consistency with NOE-based constraints and therefore further contribute to validate the structure quality. A final family of 35 conformers was calculated with RMSD values with respect to the mean structure of 0.69 +/- 0.17 A and 1.05 +/- 0.14 A for the backbone and heavy atoms, respectively. The overall fold of the molecule is maintained with respect to the native protein. The loop present between heme III and heme IV results to be highly disordered also in the present structure although its overall shape mainly resembles that of the oxidized native protein, and the two strands which give rise to the short beta-sheet present at the N-terminus and connected by a turn containing the mutated residues, are less clearly defined. If this loop is neglected, the RMSD values are 0.52 +/- 0.07 A and 0.92 +/- 0.06 A for the backbone and heavy atoms, respectively, which represent a reasonable resolution. The relative distances and orientations of the three hemes are maintained, as well as the orientation of the imidazole rings of the axial histidine ligands, with the only exception of heme IV. Such difference probably reflects minor conformational changes due to the substitution of the vicinal Lys

  8. Involvement of right STS in audio-visual integration for affective speech demonstrated using MEG.

    Science.gov (United States)

    Hagan, Cindy C; Woods, Will; Johnson, Sam; Green, Gary G R; Young, Andrew W

    2013-01-01

    Speech and emotion perception are dynamic processes in which it may be optimal to integrate synchronous signals emitted from different sources. Studies of audio-visual (AV) perception of neutrally expressed speech demonstrate supra-additive (i.e., where AV>[unimodal auditory+unimodal visual]) responses in left STS to crossmodal speech stimuli. However, emotions are often conveyed simultaneously with speech; through the voice in the form of speech prosody and through the face in the form of facial expression. Previous studies of AV nonverbal emotion integration showed a role for right (rather than left) STS. The current study therefore examined whether the integration of facial and prosodic signals of emotional speech is associated with supra-additive responses in left (cf. results for speech integration) or right (due to emotional content) STS. As emotional displays are sometimes difficult to interpret, we also examined whether supra-additive responses were affected by emotional incongruence (i.e., ambiguity). Using magnetoencephalography, we continuously recorded eighteen participants as they viewed and heard AV congruent emotional and AV incongruent emotional speech stimuli. Significant supra-additive responses were observed in right STS within the first 250 ms for emotionally incongruent and emotionally congruent AV speech stimuli, which further underscores the role of right STS in processing crossmodal emotive signals.

  9. STS-97 Pilot Bloomfield DEPARTs for Houston

    Science.gov (United States)

    2000-01-01

    After a night's rest and a brief press conference at KSC, STS-97 Pilot Michael Bloomfield heads for the plane at the Shuttle Landing Facility. With him are his wife (right) and daughter (left). They and other crew members and their families are returning to Houston. Mission STS-97 ended on Dec. 11, 2000, with a landing at KSC at 6:04 p.m. EST.

  10. Genome-wide ChIP-seq mapping and analysis of butyrate-induced H3K9 and H3K27 acetylation and epigenomic landscape alteration in bovine cells

    Science.gov (United States)

    Utilizing next-generation sequencing technology, combined with ChIP (Chromatin Immunoprecipitation) technology, we analyzed histone modification (acetylation) induced by butyrate and the large-scale mapping of the epigenomic landscape of normal histone H3 and acetylated histone H3K9 and H3K27. To d...

  11. Turning to ontology in STS? Turning to STS through ‘ontology’?

    NARCIS (Netherlands)

    van Heur, B.; Leydesdorff, L.; Wyatt, S.

    2013-01-01

    We examine the evidence for the claim of an ‘ontological turn’ in science and technology studies (STS). Despite an increase in references to ‘ontology’ in STS since 1989, we show that there has not so much been an ontological turn as multiple discussions deploying the language of ontology, consistin

  12. Turning to Ontology in STS? Turning to STS through ‘Ontology’

    NARCIS (Netherlands)

    van Heur, B.; Leydesdorff, L.; Wyatt, S.

    2012-01-01

    We examine the evidence for the claim of an ‘ontological turn’ in science and technology studies (STS). Despite an increase in references to ‘ontology’ in STS since 1989, we show that there has not so much been an ontological turn as multiple discussions deploying the language of ontology, consistin

  13. STS-90 Day 14 Highlights

    Science.gov (United States)

    1998-01-01

    On this fourteenth day of the STS-90 mission, the flight crew, Cmdr. Richard A. Searfoss, Pilot Scott D. Altman, and Mission Specialists Richard M. Linnehan, Dafydd Rhys Williams and Kathryn P. Hire, and Payload Specialists Jay C. Buckey and James A. Pawelczyk focus on the efforts of Neurolab's Neuronal Plasticity Team to better understand how the adult nervous system adapts to the new environment of space. Columbia's science crew -- Mission Specialists Rick Linnehan and Dave Williams and Payload Specialists Jay Buckey and Jim Pawelczyk -- perform the second and final in-flight dissections of the adult male rats on board. The crew euthanizes and dissects nine rats and remove the vestibular or balance organs of the inner ear; the cerebellum, the part of the brain critical for maintaining balance and for processing information from the limbs so they can be moved smoothly; and the cerebrum, one part of which controls automatic functions such as body temperature regulation and the body's internal clock, and the cortical region that controls cognitive functions such as thinking. The first dissection, which was performed on the second day of the flight, went extremely well, according to Neurolab scientists.

  14. STS-90 Day 04 Highlights

    Science.gov (United States)

    1998-01-01

    On this forth day of the STS-90 mission, the flight crew, Cmdr. Richard A. Searfoss, Pilot Scott D. Altman, and Mission Specialists Richard M. Linnehan, Dafydd Rhys Williams and Kathryn P. Hire, and Payload Specialists Jay C. Buckey and James A. Pawelczyk continue work with the Escher Staircase Behavior Testing of Adult Rats experiment. This is the first of two behavior testing sessions with the adult rats being used for this experiment. The rats will have a 'hyper drive' unit placed on their head which has recording electrodes made of microscopic wires that are positioned in the brain to record activity in the hippocampus. The hippocampus is that portion of the brain used to develop spatial maps to help us navigate from one place to the other. With the 'hyper drive' units in place, the rats will then be put through a maze or on a track. While the rat is maneuvering on the maze or track, the cell activity of the hippocampus will be measured and recorded.

  15. STS-70 Post Flight Presentation

    Science.gov (United States)

    Peterson, Glen (Editor)

    1995-01-01

    In this post-flight overview, the flight crew of the STS-70 mission, Tom Henricks (Cmdr.), Kevin Kregel (Pilot), Major Nancy Currie (MS), Dr. Mary Ellen Weber (MS), and Dr. Don Thomas (MS), discuss their mission and accompanying experiments. Pre-flight, launch, and orbital footage is followed by the in-orbit deployment of the Tracking and Data Relay Satellite (TDRS) and a discussion of the following spaceborne experiments: a microgravity bioreactor experiment to grow 3D body-like tissue; pregnant rat muscular changes in microgravity; embryonic development in microgravity; Shuttle Amateur Radio Experiment (SAREX); terrain surface imagery using the HERCULES camera; and a range of other physiological tests, including an eye and vision test. Views of Earth include: tropical storm Chantal; the Nile River and Red Sea; lightning over Brazil. A three planet view (Earth, Mars, and Venus) was taken right before sunrise. The end footage shows shuttle pre-landing checkout, entry, and landing, along with a slide presentation of the flight.

  16. STS-112 Crew Interviews: Sellers

    Science.gov (United States)

    2002-01-01

    Piers Sellers is an Astronaut from Crowborough, UK. His Bachelor of Science degree is in Ecological science from Scotland's University of Edinburgh and his doctorate is in biometeorology from Leeds University in the UK. After two years of intense training, Sellers's first assignment as a Mission Specialist is on Flight 111 STS-112. The goal of this flight is to continue building the International Space Station. Sellers, accompanied by five astronauts, will install the S1 truss of the space station which will take three EVA's, or Extra Vehicular Activities to complete. In EVA 1, the highest priority, the S1 truss will be attached to the space station. EVA 2, the electrical work, will set up the radiator and cooling equipment for the station. EVA 3, the final process of the flight, will prepare the station for the next mission. The primary reason for installing the truss is to change the center of gravity of the station so when the next truss is installed, it will be at a symmetrical point.

  17. STS, symmetry and post-truth.

    Science.gov (United States)

    Lynch, Michael

    2017-08-01

    This essay takes up a series of questions about the connection between 'symmetry' in Science and Technology Studies (STS) and 'post-truth' in contemporary politics. A recent editorial in this journal by Sergio Sismondo argues that current discussions of 'post-truth' have little to do with conceptions of 'symmetry' or with concerns about 'epistemic democracy' in STS, while others, such as Steve Fuller and Harry Collins, insist that there are such connections. The present essay discusses a series of questions about the meaning of 'post-truth' and 'symmetry', and the connections of those concepts to each other and to 'epistemic democracy'. The essay ends with a series of other questions about STS and contemporary politics, and an invitation to further discussions.

  18. 基于Oracle和MapGIS K9平台的第二次土地调查(农村部分)市级数据库建设——以崇左市为例

    Institute of Scientific and Technical Information of China (English)

    陈兰康; 何钊

    2012-01-01

    市级数据库(农村部分)建设是第二次全国土地调查的一项重要内容,是国土资源信息化的基础,是实现国土资源管理方式根本转变和国土资源工作现代化的重要组成部分。本文以Oracle 11G、MapGIS K9平台为基础,数据库建库、管理系统平台采用MapGIS K9国土资源数据中心系统,完成崇左市第二次土地调查(农村部分)数据库建设工作。

  19. Effect of Foliage Top-dressing of Potassium on the Photosynthetic Rate of Apple Trees%根外施钾对K9苹果树光合速率的影响研究

    Institute of Scientific and Technical Information of China (English)

    徐立祥; 唐雪东; 刘晓嘉

    2010-01-01

    采用GPS-1便携式光合作用测定系统,研究K9苹果树生育期根外施K对其光合速率的影响.结果表明:K9苹果树光合速率日变化呈双峰曲线,且根外施K处理叶片净光合速率(Pn)明显高于对照,在一定程度可缓和"午休"现象,每次喷钾的作用可持续25 d左右.并且3次根外施K处理叶片光合速率明显高于根外1次施K和2次施K处理.

  20. Photoionization study of Ne-like K9+, Ca10+, Sc11+, Ti12+, V13+, Cr14+, Mn15+, and Fe16+ ions using the screening constant by unit nuclear charge method

    Science.gov (United States)

    Goyal, Arun; Khatri, Indu; Sow, Malick; Sakho, Ibrahima; Aggarwal, Sunny; Singh, A. K.; Mohan, Man

    2016-08-01

    Photoionization of the 2s22p6 (1S0) ground state of the Ne-like (Z=19-29) ions is presented in this paper. Resonance energies and total natural width of the 2s2p6np 1P series of the Ne-like K9+, Ca10+, Sc11+, Ti12+, V13+, Cr14+, Mn15+, and Fe16+are reported. All the calculations are made using the Screening constant by unit nuclear charge (SCUNC) formalism. New data for Ne-like K9+, Sc11+, Ti12+, V13+, Cr14+, and Mn15+ions are tabulated. Good agreements are found with available literature data.

  1. A CO32--containing, dimanganese-substituted silicotungstate trimer, K9[H14{SiW10MnIIMnIIIO38}3(CO3)]·39H2O.

    Science.gov (United States)

    Yang, Ling; Liu, Qisen; Ma, Pengtao; Niu, Jingyang; Wang, Jingping

    2015-08-14

    An unprecedented silicotungstate trimer K(9)[H(14){SiW(10)Mn(II)Mn(III)O(38)}(3)(CO(3))]·39H(2)O (1) has been successfully synthesized, in which the CO(3)(2-) resides inside the three Keggin {SiW(10)Mn(II)Mn(III)O(38)} units and the three O atoms serve as μ(2)-O atoms to connect with three Mn(III). Magnetic investigation indicates that 1 exhibits antiferromagnetic coupling.

  2. Is DOG1 really useful in the diagnosis of salivary gland acinic cell carcinoma? - A DOG1 (clone K9 analysis in fine needle aspiration cell blocks and the review of the literature

    Directory of Open Access Journals (Sweden)

    Sule Canberk

    2015-01-01

    Full Text Available Introduction: DOG1 is a transmembrane protein originally "discovered on gastrointestinal stromal tumors," works as a calcium-activated chloride channel protein. There is a limited number of studies on the potential usage of this antibody in the diagnosis of salivary gland tumors on routine practice in cell blocks. The aim of this study was to search for the usefulness of K9 clone in oncocytic type tumors and review of the literature. Materials and Methods: Sixty-nine fine needle aspiration (FNA cytologic materials of predominantly oncocytic morphology salivary gland tumors; acinic cell carcinoma (AciCC (n = 8, adenoid cystic carcinoma (n = 2, pleomorphic adenoma (PA (n = 22, Warthin tumor (WT (n = 20, myoepithelioma (ME (n = 5, benign oncocytoma (BeO (n = 3, mucoepidermoid carcinoma (MEC (n = 7, mammary analog salivary gland carcinoma (n = 2 were immunostained with DOG1 (clone K9 stain. Results: Of the 8 AciCCs, 7 were observed apical-luminal positive staining, demonstrating 1-3 + intensity, and involving 40-70% of the tumor cells. One MEC of 7 (14%, 1 ME of 5 (20%, and 4 PA of 22 (18% showed weak (1+ cytoplasmic granular staining in 5-10% of the tumor cells. Pure oncocytic neoplasms (WT, BeO showed no expression with DOG1-K9. Conclusions: FNA is a common tool in the diagnosis and management of salivary gland tumors. DOG1-K9 clone was very useful with a unique staining pattern of apical-luminal positivity in the differential diagnosis of AciCC from other oncocytic salivary gland tumors.

  3. STS-104 Crew Interview: Steve Lindsey

    Science.gov (United States)

    2001-01-01

    STS-104 Commander Steve Lindsey is seen being interviewed. He answers questions about his inspiration to become an astronaut and his career path. He gives details on the mission's goals and significance, its payload (the Joint Airlock and the external gas tanks), and the usefulness of the newly installed Canadian Robotic Arm (installed by STS-100 crew). Lindsey describes his role in the rendezvous, docking, undocking, and flyaround of the Atlantis Orbiter and the International Space Station (ISS) and discusses the mission's planned spacewalks.

  4. Live Worms Found Amid STS-107 Debris

    Science.gov (United States)

    2003-01-01

    NASA Project Manager Fred Ahmay holds a Biological Research in Canisters (BRIC) container in which C. elegans nemotodes (round worms) were found. The container was part of a middeck experiment that was among Columbia's debris recovered in East Texas. The worms were found alive after flying on Columbia's last mission, STS-107. The experiment was designed to verify a new synthetic nutrient solution for an International Space Station 'model' specimen planned to be used extensively for ISS gene expression studies and was sponsored by the NASA Ames Research Center. For more information on STS-107, please see GRIN Columbia General Explanation

  5. STS-110 Commander Bloomfield arrives for TCDT

    Science.gov (United States)

    2002-01-01

    KENNEDY SPACE CENTER, FLA. -- STS-110 Commander Michael Bloomfield waves as he gets ready to depart KSC for Houston. He and the rest of the crew were at KSC for Terminal Countdown Demonstration Test activities that included payload familiarization and a simulated launch countdown. Scheduled for launch April 4, the 11-day STS-110 mission will feature Space Shuttle Atlantis docking with the International Space Station (ISS) and delivering the S0 truss, the centerpiece-segment of the primary truss structure that will eventually extend over 300 feet.

  6. Early postnatal feed restriction reduces liver connective tissue levels and affects H3K9 acetylation state of regulated genes associated with protein metabolism in low birth weight pigs.

    Science.gov (United States)

    Nebendahl, Constance; Görs, Solvig; Albrecht, Elke; Krüger, Ricarda; Martens, Karen; Giller, Katrin; Hammon, Harald M; Rimbach, Gerald; Metges, Cornelia C

    2016-03-01

    Intrauterine growth retardation is associated with metabolic consequences in adulthood. Since our previous data indicate birth weight-dependent effects of feed restriction (R) on protein degradation processes in the liver, it should be investigated whether effects on connective tissue turnover are obvious and could be explained by global changes of histone H3K9me3 and H3K9ac states in regulated genes. For this purpose, female littermate pigs with low (U) or normal (N) birth weight were subjected to 3-week R (60% of ad libitum fed controls) with subsequent refeeding (REF) for further 5 weeks. The 3-week R-period induced a significant reduction of connective tissue area by 43% in the liver of U animals at 98 d of age, which was not found in age-matched N animals. Of note, after REF at 131 d of age, in previously feed-restricted U animals (UR), the percentage of mean connective tissue was only 53% of ad libitum fed controls (UK), indicating a persistent effect. In U animals, R induced H3K9 acetylation of regulated genes (e.g. XBP1, ERLEC1, GALNT2, PTRH2), which were inter alia associated with protein metabolism. In contrast, REF was mostly accompanied by deacetylation in U and N animals. Thus, our epigenetic data may give a first explanation for the observed birth weight-dependent differences in this connective tissue phenotype.

  7. Selective interactions between vertebrate polycomb homologs and the SUV39H1 histone lysine methyltransferase suggest that histone H3-K9 methylation contributes to chromosomal targeting of Polycomb group proteins.

    Science.gov (United States)

    Sewalt, Richard G A B; Lachner, Monika; Vargas, Mark; Hamer, Karien M; den Blaauwen, Jan L; Hendrix, Thijs; Melcher, Martin; Schweizer, Dieter; Jenuwein, Thomas; Otte, Arie P

    2002-08-01

    Polycomb group (PcG) proteins form multimeric chromatin-associated protein complexes that are involved in heritable repression of gene activity. Two distinct human PcG complexes have been characterized. The EED/EZH2 PcG complex utilizes histone deacetylation to repress gene activity. The HPC/HPH PcG complex contains the HPH, RING1, BMI1, and HPC proteins. Here we show that vertebrate Polycomb homologs HPC2 and XPc2, but not M33/MPc1, interact with the histone lysine methyltransferase (HMTase) SUV39H1 both in vitro and in vivo. We further find that overexpression of SUV39H1 induces selective nuclear relocalization of HPC/HPH PcG proteins but not of the EED/EZH2 PcG proteins. This SUV39H1-dependent relocalization concentrates the HPC/HPH PcG proteins to the large pericentromeric heterochromatin domains (1q12) on human chromosome 1. Within these PcG domains we observe increased H3-K9 methylation. Finally, we show that H3-K9 HMTase activity is associated with endogenous HPC2. Our findings suggest a role for the SUV39H1 HMTase and histone H3-K9 methylation in the targeting of human HPC/HPH PcG proteins to modified chromatin structures.

  8. STS-86 Pilot Mike Bloomfield speaks at TCDT

    Science.gov (United States)

    1997-01-01

    STS-86 Pilot Michael J. Bloomfield speaks to media representatives and other onlookers during Terminal Countdown Demonstration Test (TCDT) activities at Launch Pad 39A. This will be his first spaceflight. STS-86 will be the seventh docking of the Space Shuttle with the Russian Space Station Mir. During the docking, STS-86 Mission Specialist David A. Wolf will transfer to the orbiting Russian station and become a member of the Mir 24 crew, replacing U.S. astronaut C. Michael Foale, who has been on the Mir since the last docking mission, STS-84, in May. Launch of Mission STS-86 aboard the Space Shuttle Atlantis is targeted for Sept. 25.

  9. Pharmaceutical experiment aboard STS-67 mission

    Science.gov (United States)

    1995-01-01

    Astronaut William G. Gregory, pilot, works with a pharmaceutical experiment on the middeck of the Earth-orbiting Space Shuttle Endeavour during the STS-67 mission. Commercial Materials Dispersion Apparatus Instruments Technology Associates Experiments (CMIX-03) includes not only pharmaceutical, but also biotechnology, cell biology, fluids, and crystal growth investigation

  10. STS-107 Flight Day 5 Highlights

    Science.gov (United States)

    2003-01-01

    The fifth day of the STS-107 space mission begins with a presentation of The Six Space Technology and Research Students (STARS) program experiments aboard the Space Shuttle Columbia. Students from Australia, China, Israel, Japan, Lichtenstein and The United States send scientific experiments into space. The video includes the progress of experiments with various insects including silkworms, carpenter bees, ants, fish, and spiders.

  11. STS-98 Crew Interview: Bob Curbeam

    Science.gov (United States)

    2001-01-01

    The STS-98 Mission Specialist Bob Curbeam is seen being interviewed. He answers questions about his inspiration to become an astronaut, his career path, and his training. He gives details on the mission's goals and significance, and the payload and hardware it brings to the International Space Station (ISS). Mr. Curbeam discusses his role in the mission's spacewalks and activities.

  12. STS-98 Crew Interview: Tom Jones

    Science.gov (United States)

    2001-01-01

    The STS-98 Mission Specialist Tom Jones is seen being interviewed. He answers questions about his inspiration to become an astronaut, his career path, and his training. He gives details on the mission's goals and significance, and the payload and hardware it brings to the International Space Station (ISS). Mr. Jones discusses his role in the mission's spacewalks and activities.

  13. STS Motion Control Using Humanoid Robot

    Directory of Open Access Journals (Sweden)

    Mohd Bazli Bahar

    2014-07-01

    Full Text Available This study presents the development of Sit to Stand (STS motion control method. The main challenge in STS is in addressing the lift-off from chair problem. In solving the problem, the main components of the humanoid STS motion system involved are the (1 phase and trajectory planning and (2 motion control. These components should be designed so that the Zero Moment Point (ZMP, Centre of Pressure (CoP and Centre of Mass (CoM is always in the support polygon. Basically, in STS motion control there are two components, 1. Action selector and 2. Tracking controller. The STS motion control should able to operate in real time and continuously able to adapt any change in between the motion. In this way, the accuracy of the controller to rectify the motion error shall increase. The overall proposed method to perform the STS motion is designed to have two main phases. (1 CoM transferring that implements Alexander STS technique and (2 Stabilization Strategy that used IF-THEN rules and proportional velocity controller. This study focuses on the presentation of the development of second phase which are 1. The development of the IF-THEN rules as the action selector that operates in real time to assists the proportional controller in making the best decision and, 2. The development of Proportional Gain Identification for the proportional velocity controller that is capable to change the gain implementation by referring to the define region that represent the motion condition. The validation of the proposed method is done experimentally using NAO robot as the test platform. The coefficient of the gain identification for the proportional controller was tuned using NAO robot that was initially set at sitting position on a wooden chair. The inclination of the body from a frame perpendicular with the ground, angle y is observed. Coefficient that gives the lowest RMSE of angle y trajectory is taken as a constant. Results show the proposed control method has reduce

  14. STS Case Study Development Support

    Science.gov (United States)

    Rosa de Jesus, Dan A.; Johnson, Grace K.

    2013-01-01

    The Shuttle Case Study Collection (SCSC) has been developed using lessons learned documented by NASA engineers, analysts, and contractors. The SCSC provides educators with a new tool to teach real-world engineering processes with the goal of providing unique educational materials that enhance critical thinking, decision-making and problem-solving skills. During this third phase of the project, responsibilities included: the revision of the Hyper Text Markup Language (HTML) source code to ensure all pages follow World Wide Web Consortium (W3C) standards, and the addition and edition of website content, including text, documents, and images. Basic HTML knowledge was required, as was basic knowledge of photo editing software, and training to learn how to use NASA's Content Management System for website design. The outcome of this project was its release to the public.

  15. Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) replication and transcription factor activates the K9 (vIRF) gene through two distinct cis elements by a non-DNA-binding mechanism.

    Science.gov (United States)

    Ueda, Keiji; Ishikawa, Kayo; Nishimura, Ken; Sakakibara, Shuhei; Do, Eunju; Yamanishi, Koichi

    2002-12-01

    The replication and transcription activator (RTA) of Kaposi's sarcoma-associated herpesvirus (KSHV), or human herpesvirus 8, a homologue of Epstein-Barr virus BRLF1 or Rta, is a strong transactivator and inducer of lytic replication. RTA acting alone can induce lytic replication of KSHV in infected cell lines that originated from primary effusion lymphomas, leading to virus production. During the lytic replication process, RTA activates many kinds of genes, including polyadenylated nuclear RNA, K8, K9 (vIRF), ORF57, and so on. We focused here on the mechanism of how RTA upregulates the K9 (vIRF) promoter and identified two independent cis-acting elements in the K9 (vIRF) promoter that responded to RTA. These elements were finally confined to the sequence 5'-TCTGGGACAGTC-3' in responsive element (RE) I-2B and the sequence 5'-GTACTTAAAATA-3' in RE IIC-2, both of which did not share sequence homology. Multiple factors bound specifically with these elements, and their binding was correlated with the RTA-responsive activity. Electrophoretic mobility shift assay with nuclear extract from infected cells and the N-terminal part of RTA expressed in Escherichia coli, however, did not show that RTA interacted directly with these elements, in contrast to the RTA responsive elements in the PAN/K12 promoter region, the ORF57/K8 promoter region. Thus, it was likely that RTA could transactivate several kinds of unique cis elements without directly binding to the responsive elements, probably through cooperation with other DNA-binding factors.

  16. 5-Aza-2'-deoxycytidine-mediated reductions in G9A histone methyltransferase and histone H3 K9 di-methylation levels are linked to tumor suppressor gene reactivation.

    Science.gov (United States)

    Wozniak, R J; Klimecki, W T; Lau, S S; Feinstein, Y; Futscher, B W

    2007-01-04

    The epigenetic silencing of tumor suppressor genes is a common event during carcinogenesis, and often involves aberrant DNA methylation and histone modification of gene regulatory regions, resulting in the formation of a transcriptionally repressive chromatin state. Two examples include the antimetastatic, tumor suppressor genes, desmocollin 3 (DSC3) and MASPIN, which are frequently silenced in this manner in human breast cancer. Treatment of the breast tumor cell lines MDA-MB-231 and UACC 1179 with 5-aza-2'-deoxycytidine (5-aza-CdR) induced transcriptional reactivation of both genes in a dose-dependent manner. Importantly, DSC3 and MASPIN reactivation was closely and consistently linked with significant decreases in promoter H3 K9 di-methylation. Moreover, 5-aza-CdR treatment also resulted in global decreases in H3 K9 di-methylation, an effect that was linked to its ability to mediate dose-dependent, post-transcriptional decreases in the key enzyme responsible for this epigenetic modification, G9A. Finally, small interfering RNA (siRNA)-mediated knockdown of G9A and DNMT1 led to increased MASPIN expression in MDA-MB-231 cells, to levels that were supra-additive, verifying the importance of these enzymes in maintaining multiple layers of epigenetic repression in breast tumor cells. These results highlight an additional, complimentary mechanism of action for 5-aza-CdR in the reactivation of epigenetically silenced genes, in a manner that is independent of its effects on DNA methylation, further supporting an important role for H3 K9 methylation in the aberrant repression of tumor suppressor genes in human cancer.

  17. STS-110 Flight Day 8 Highlights

    Science.gov (United States)

    2002-01-01

    On Flight Day 8 of the STS-110 mission, the combined members of the Atlantis STS-110 crew and the International Space Station (ISS) Expedition 4 crew, Commanders Michael J. Bloomfield and Yury I. Onufrienko, respectively, answer questions posed to them by press members on the ground. Topics covered include: challenges of long term space habitation, adequacy of training for the on-orbit construction of the space station, details on the Mobile Transporter (MT) railcar system. Footage of the MT directly precedes and follows the extended question and answer portion of the video. The MT is seen from a variety of angles, as it slowly moves from Workstation 4 to Workstation 5 in order to test its ability to grapple to a fixed point along the track. The MT will eventually be used as a base for the robot arm in order to perform maintenance and assembly tasks on the ISS.

  18. STS flight operations - Concept versus reality

    Science.gov (United States)

    Kranz, E. F.

    1985-01-01

    The NASA Lyndon B. Johnson Space Center Mission Operations Directorate (MOD) has the responsibility for planning, training, and implementing of flight operations in support of the National Space Transportation System (STS) Program. In this role, MOD has responsibility for the operational interface with customers and the translation of customer requirements into operating plans to satisfy their objectives. The basic objectives of flight operations are to maximize mission success, to minimize risks to the Orbiter and the crew, to decrease operating costs, and to achieve an effective balance in the application of all operational resources. In the National STS Program, to date, 20 missions have been flown and the process for planning, training, and flight operations has been thoroughly exercised. In this paper, MOD's performance is assessed, and those areas in which our initial operating concepts have been successful as well as those in which significant additional work is required are identified.

  19. Former astronaut Armstrong witnesses STS-83 launch

    Science.gov (United States)

    1997-01-01

    Apollo l1 Commander Neil A. Armstrong and his wife, Carol, were among the many special NASA STS-83 launch guests who witnessed the liftoff of the Space Shuttle Columbia April 4 at the Banana Creek VIP Viewing Site at KSC. Columbia took off from Launch Pad 39A at 2:20:32 p.m. EST to begin the 16-day Microgravity Science Laboratory-1 (MSL-1) mission.

  20. STS-100 Crew Interview: Jeff Ashby

    Science.gov (United States)

    2001-01-01

    STS-100 Pilot Jeff Ashby is seen being interviewed. He answers questions about his inspiration to become an astronaut and his career path. He gives details on the mission's goals and significance, the rendezvous and docking of Endeavour with the International Space Station (ISS), the mission's spacewalks, and installation and capabilities of the Space Station robotic arm, UHF antenna, and Rafaello Logistics Module. Ashby then discusses his views about space exploration as it becomes an international collaboration.

  1. STS-64 SAFER Assembly development team members

    Science.gov (United States)

    1993-01-01

    The Simplified Aid for EVA Rescue (SAFER) system, to be tested on the STS-64 flight, is surrounded by the team members who have spent a number of recent man hours in preparation for the system's first test-flight. In front are (left to right) Russell L. Flack and Bob Lowe. In the back row are (left to right) Jack D. Humphreys, Chuck Deason, Bill Wood and James Brown.

  2. STS-47 Astronaut Crew Training Clip

    Science.gov (United States)

    1992-01-01

    The crew of STS-47, Commander Robert L. Gibson, Pilot Curtis L. Brown, Payload Commander Mark C. Lee, Mission Specialists N. Jan Davis, Jay Apt, and Mae C. Jemison, and Payload Specialist Mamoru Mohri, is seen during various parts of their training, including SAREX training in the Full Fuselage Trainer (FFT), firefighting training. A familiarization flight in the KC-135, a food tasting, photo training in the Crew Compartment Trainer, and bailout training in the Weightless Environment Training Facility (WETF) are also shown.

  3. STS-86 Pilot Michael Bloomfield suits up

    Science.gov (United States)

    1997-01-01

    STS-86 Pilot Michael J. Bloomfield relaxes for a moment while donning his launch and entry suit with the assistance of a suit technician in the Operations and Checkout Building. This will be Bloomfields first spaceflight. He and the six other crew members will depart shortly for Launch Pad 39A, where the Space Shuttle Atlantis awaits liftoff on a 10-day mission slated to be the seventh docking of the Space Shuttle with the Russian Space Station Mir.

  4. STS-96 Astronauts Adjust Unity Hatch

    Science.gov (United States)

    1999-01-01

    Aboard the International Space Station (ISS), astronauts Rick D. Husband and Tamara E. Jernigan adjust the hatch for the U.S. built Unity node. The task was part of an overall effort of seven crew members to prepare the existing portion of the International Space Station (ISS). Launched on May 27, 1999, aboard the Orbiter Discovery, the STS-96 mission was the second ISS assembly flight and the first shuttle mission to dock with the station.

  5. STS-100 Crew Interview: John Phillips

    Science.gov (United States)

    2001-01-01

    STS-100 Mission Specialist John Phillips is seen being interviewed. He answers questions about his inspiration to become an astronaut and his career path. He gives details on the mission's goals and significance, the rendezvous and docking of Endeavour with the International Space Station (ISS), the mission's spacewalks, and installation and capabilities of the Space Station robotic arm, UHF antenna, and Rafaello Logistics Module. Phillips then discusses his views about space exploration as it becomes an international collaboration.

  6. 农村集体土地所有权登记发证MAPGIS K9入库前的框架搭建方法步骤

    Institute of Scientific and Technical Information of China (English)

    连金玲; 郭秋云; 惠以珩

    2013-01-01

    农村集体土地所有权工作入库工作是必不可少的一项内容,而枣庄市农村集体土地确权外业完成后的内业成图都是用的南方CASS8.0软件就,本文主要针对枣庄市农村集体土地所有权,利用MAPGIS K9入库前,由CASS8.0到MAGIS的入库准备工作,包括界址点、界址线转换到MAPGIS下,以及界址点、界址线、宗地属性的录入和挂接,为入库奠定良好的基础。框架搭建好后直接导入MAPGIS K9中完善库的内容。

  7. STS-69 Clears the tower (side view)

    Science.gov (United States)

    1995-01-01

    A pack of astronauts that run under the banner Dog Crew II heads for the stars. Liftoff of the Space Shuttle Endeavour from Launch Pad 39A occurred at 11:09:00.052 a.m. EDT, Sept. 7, 1995. 'Every dog has its day and today is your day,' KSC Orbiter Test Conductor Roger Gillette pledged to STS-69 Mission Commander David M. Walker, Pilot Kenneth D. Cockrell, Payload Commander James S. Voss and Mission Specialists Michael L. Gernhardt and James H. Newman prior to launch. The STS-69 astronaut crew developed a strong sense of comaraderie as they went through their flight training, and dubbed themselves the Dog Crew II to carry on a tradition that arose during an earlier Shuttle flight -- STS-53 -- to which both Voss and Walker were assigned. Each crew member adopted a dog-theme name: Walker is Red Dog; Cockrell, Cujo; Voss, Dogface; Newman, Pluto; and Gernhardt, the only space rookie, Underdog. A special patch, featuring a bulldog in a doghouse shaped like the Space Shuttle, was designed for the astronauts and other flight team members to wear. The Dog Crew II is embarking on an 11-day multifaceted mission featuring two free-flying scientific research spacecraft as well as a host of experiments in both the payload bay and the middeck. Also scheduled is an extravehicular activity, or spacewalk.

  8. Astronaut Sellers Performs STS-112 EVA

    Science.gov (United States)

    2002-01-01

    Launched October 7, 2002 aboard the Space Shuttle Orbiter Atlantis, the STS-112 mission lasted 11 days and performed three sessions of Extra Vehicular Activity (EVA). Its primary mission was to install the Starboard Side Integrated Truss Structure (S1) and Equipment Translation Aid (CETA) Cart to the International Space Station (ISS). The S1 truss provides structural support for the orbiting research facility's radiator panels, which use ammonia to cool the Station's complex power system. The S1 truss, attached to the S0 (S Zero) truss installed by the previous STS-110 mission, flows 637 pounds of anhydrous ammonia through three heat rejection radiators. The truss is 45-feet long, 15-feet wide, 10-feet tall, and weighs approximately 32,000 pounds. The CETA is the first of two human-powered carts that will ride along the International Space Station's railway providing a mobile work platform for future extravehicular activities by astronauts. In this photograph, Astronaut Piers J. Sellers uses both a handrail on the Destiny Laboratory and a foot restraint on the Space Station Remote Manipulator System or Canadarm2 to remain stationary while performing work at the end of the STS-112 mission's second space walk. A cloud-covered Earth provides the backdrop for the scene.

  9. Computational Thinking in K-9 Education

    NARCIS (Netherlands)

    Mannila, Linda; Dagiene, Valentina; Demo, Barbara; Grgurina, Natasa; Mirolo, Claudio; Rolandsson, Lennart; Settle, Amber

    2014-01-01

    In this report we consider the current status of the coverage of computer science in education at the lowest levels of education in multiple countries. Our focus is on computational thinking (CT), a term meant to encompass a set of concepts and thought processes that aid in formulating problems and

  10. Transferability of STS markers in studying genetic relationships of marvel grass (Dichanthium annulatum).

    Science.gov (United States)

    Saxena, Raghvendra; Chandra, Amaresh

    2011-11-01

    Transferability of sequence-tagged-sites (STS) markers was assessed for genetic relationships study among accessions of marvel grass (Dichanthium annulatum Forsk.). In total, 17 STS primers of Stylosanthes origin were tested for their reactivity with thirty accessions of Dichanthium annulatum. Of these, 14 (82.4%) reacted and a total 106 (84 polymorphic) bands were scored. The number of bands generated by individual primer pairs ranged from 4 to 11 with an average of 7.57 bands, whereas polymorphic bands ranged from 4 to 9 with an average of 6.0 bands accounts to an average polymorphism of 80.1%. Polymorphic information content (PIC) ranged from 0.222 to 0.499 and marker index (MI) from 1.33 to 4.49. Utilizing Dice coefficient of genetic similarity dendrogram was generated through un-weighted pairgroup method with arithmetic mean (UPGMA) algorithm. Further, clustering through sequential agglomerative hierarchical and nested (SAHN) method resulted three main clusters constituted all accessions except IGBANG-D-2. Though there was intermixing of few accessions of one agro-climatic region to another, largely groupings of accessions were with their regions of collections. Bootstrap analysis at 1000 scale also showed large number of nodes (11 to 17) having strong clustering (> 50). Thus, results demonstrate the utility of STS markers of Stylosanthes in studying the genetic relationships among accessions of Dichanthium.

  11. H2A.Z demarcates intergenic regions of the plasmodium falciparum epigenome that are dynamically marked by H3K9ac and H3K4me3.

    Directory of Open Access Journals (Sweden)

    Richárd Bártfai

    Full Text Available Epigenetic regulatory mechanisms and their enzymes are promising targets for malaria therapeutic intervention; however, the epigenetic component of gene expression in P. falciparum is poorly understood. Dynamic or stable association of epigenetic marks with genomic features provides important clues about their function and helps to understand how histone variants/modifications are used for indexing the Plasmodium epigenome. We describe a novel, linear amplification method for next-generation sequencing (NGS that allows unbiased analysis of the extremely AT-rich Plasmodium genome. We used this method for high resolution, genome-wide analysis of a histone H2A variant, H2A.Z and two histone H3 marks throughout parasite intraerythrocytic development. Unlike in other organisms, H2A.Z is a constant, ubiquitous feature of euchromatic intergenic regions throughout the intraerythrocytic cycle. The almost perfect colocalisation of H2A.Z with H3K9ac and H3K4me3 suggests that these marks are preferentially deposited on H2A.Z-containing nucleosomes. By performing RNA-seq on 8 time-points, we show that acetylation of H3K9 at promoter regions correlates very well with the transcriptional status whereas H3K4me3 appears to have stage-specific regulation, being low at early stages, peaking at trophozoite stage, but does not closely follow changes in gene expression. Our improved NGS library preparation procedure provides a foundation to exploit the malaria epigenome in detail. Furthermore, our findings place H2A.Z at the cradle of P. falciparum epigenetic regulation by stably defining intergenic regions and providing a platform for dynamic assembly of epigenetic and other transcription related complexes.

  12. Interactions between beta D372 and gamma subunit N-terminus residues gamma K9 and gamma S12 are important to catalytic activity catalyzed by Escherichia coli F1F0-ATP synthase.

    Science.gov (United States)

    Lowry, David S; Frasch, Wayne D

    2005-05-17

    Substitution of Escherichia coli F(1)F(0) ATP synthase residues betaD372 or gammaS12 with groups that are unable to form a hydrogen bond at this location decreased ATP synthase-dependent cell growth by 2 orders of magnitude, eliminated the ability of F(1)F(0) to catalyze ATPase-dependent proton pumping in inverted E. coli membranes, caused a 15-20% decrease in the coupling efficiency of the membranes as measured by the extent of succinate-dependent acridine orange fluorescence quenching, but increased soluble F(1)-ATPase activity by about 10%. Substitution of gammaK9 to eliminate the ability to form a salt bridge with betaD372 decreased soluble F(1)-ATPase activity and ATPase-driven proton pumping by 2-fold but had no effect on the proton gradient induced by addition of succinate. Mutations to eliminate the potential to form intersubunit hydrogen bonds and salt bridges between other less highly conserved residues on the gamma subunit N-terminus and the beta subunits had little effect on ATPase or ATP synthase activities. These results suggest that the betaD372-gammaK9 salt bridge contributes significantly to the rate-limiting step in ATP hydrolysis of soluble F(1) while the betaD372-gammaS12 hydrogen bond may serve as a component of an escapement mechanism for ATP synthesis in which alphabetagamma intersubunit interactions provide a means to make substrate binding a prerequisite of proton gradient-driven gamma subunit rotation.

  13. A cullin E3 ubiquitin ligase complex associates with Rik1 and the Clr4 histone H3-K9 methyltransferase and is required for RNAi-mediated heterochromatin formation.

    Science.gov (United States)

    Hong, Eun-Jin Erica; Villén, Judit; Gerace, Erica L; Gygi, Steven P; Moazed, Danesh

    2005-01-01

    The assembly of heterochromatin in fission yeast and metazoans requires histone H3-lysine 9 (-K9) methylation by the conserved Clr4/Suv39h methyltransferase. In fission yeast, H3-K9 methylation requires components of the RNAi machinery and is initiated by the RNA-Induced Transcriptional Silencing (RITS) complex. Here we report the purification of a novel complex that associates with the Clr4 methyltransferase, termed the CLRC (CLr4-Rik1-Cul4) complex. By affinity purification of the Clr4-associated protein Rik1, we show that, in addition to Clr4, Rik1 is associated with the fission yeast E3 ubiquitin ligase Cullin4 (Cul4, encoded by cul4(+)), the ubiquitin-like protein, Ned8, and two previously uncharacterized proteins, designated Cmc1 and Cmc2. In addition, the complex contains substochiometric amounts of histones H2B and H4, and the 14-3-3 protein, Rad24. Deletion of cul4(+), cmc1(+), cmc2(+) and rad24(+) results in a complete loss of silencing of a ura4(+) reporter gene inserted within centromeric DNA repeats or the silent mating type locus. Each of the above deletions also results in accumulation of noncoding RNAs transcribed from centromeric repeats and telomeric DNA regions, and a corresponding loss of small RNAs that are homologous to centromeric repeats, suggesting a defect in the processing of noncoding RNA to small RNA. Based on these results, we propose that the components of the Clr4-Rik1-Cul4 complex act concertedly at an early step in heterochromatin formation.

  14. STS-61 Space Shuttle mission report

    Science.gov (United States)

    Fricke, Robert W., Jr.

    1994-02-01

    The STS-61 Space Shuttle Program Mission Report summarizes the Hubble Space Telescope (HST) servicing mission as well as the Orbiter, External Tank (ET), Solid Rocket Booster (SRB), Redesigned Solid Rocket Motor (RSRM), and the Space Shuttle main engine (SSME) systems performance during the fifty-ninth flight of the Space Shuttle Program and fifth flight of the Orbiter vehicle Endeavour (OV-105). In addition to the Orbiter, the flight vehicle consisted of an ET designated as ET-60; three SSME's which were designated as serial numbers 2019, 2033, and 2017 in positions 1, 2, and 3, respectively; and two SRB's which were designated BI-063. The RSRM's that were installed in each SRB were designated as 360L023A (lightweight) for the left SRB, and 360L023B (lightweight) for the right SRB. This STS-61 Space Shuttle Program Mission Report fulfills the Space Shuttle Program requirement as documented in NSTS 07700, Volume 8, Appendix E. That document requires that each major organizational element supporting the Program report the results of its hardware evaluation and mission performance plus identify all related in-flight anomalies. The primary objective of the STS-61 mission was to perform the first on-orbit servicing of the Hubble Space Telescope. The servicing tasks included the installation of new solar arrays, replacement of the Wide Field/Planetary Camera I (WF/PC I) with WF/PC II, replacement of the High Speed Photometer (HSP) with the Corrective Optics Space Telescope Axial Replacement (COSTAR), replacement of rate sensing units (RSU's) and electronic control units (ECU's), installation of new magnetic sensing systems and fuse plugs, and the repair of the Goddard High Resolution Spectrometer (GHRS). Secondary objectives were to perform the requirements of the IMAX Cargo Bay Camera (ICBC), the IMAX Camera, and the Air Force Maui Optical Site (AMOS) Calibration Test.

  15. STS-62 Space Shuttle mission report

    Science.gov (United States)

    Fricke, Robert W., Jr.

    1994-05-01

    The STS-62 Space Shuttle Program Mission Report summarizes the Payload activities as well as the Orbiter, External Tank (ET), Solid Rocket Booster (SRB), Redesigned Solid Rocket Motor (RSRM), and the Space Shuttle main engine (SSHE) systems performance during the sixty-first flight of the Space Shuttle Program and sixteenth flight of the Orbiter vehicle Columbia (OV-102). In addition to the Orbiter, the flight vehicle consisted of an ET designated as ET-62; three SSME's which were designated as serial numbers 2031, 2109, and 2029 in positions 1, 2, and 3, respectively; and two SRB's which were designated BI-064. The RSRM's that were installed in each SRB were designated as 360L036A (lightweight) for the left SRB, and 36OWO36B (welterweight) for the right SRB. This STS-62 Space Shuttle Program Mission Report fulfills the Space Shuttle Program requirement as documented in NSTS 07700, Volume 8, Appendix E. That document requires that each major organizational element supporting the Program report the results of its hardware evaluation and mission performance plus identify all related in-flight anomalies. The primary objectives of the STS-62 mission were to perform the operations of the United States Microgravity Payload-2 (USMP-2) and the Office of Aeronautics and Space Technology-2 (OAST-2) payload. The secondary objectives of this flight were to perform the operations of the Dexterous End Effector (DEE), the Shuttle Solar Backscatter Ultraviolet/A (SSBUV/A), the Limited Duration Space Environment Candidate Material Exposure (LDCE), the Advanced Protein Crystal Growth (APCG), the Physiological Systems Experiments (PSE), the Commercial Protein Crystal Growth (CPCG), the Commercial Generic Bioprocessing Apparatus (CGBA), the Middeck Zero-Gravity Dynamics Experiment (MODE), the Bioreactor Demonstration System (BDS), the Air Force Maui Optical Site Calibration Test (AMOS), and the Auroral Photography Experiment (APE-B).

  16. STS-112 Commander Ashby during post landing news conference

    Science.gov (United States)

    2002-01-01

    KENNEDY SPACE CENTER, FLA. - STS-112 Commander Jeffrey Ashby is shown during the crew's post-landing briefing for the media. Mission STS-112 was the 15th assembly flight to the International Space Station, installing the S1 truss. The landing was the 60th at KSC in the history of the Shuttle program.

  17. STS-129 shuttle crew visits Stennis

    Science.gov (United States)

    2010-01-01

    Members of the STS-129 space shuttle crew visited NASA's John C. Stennis Space Center on Jan. 19 to share details of their November visit to the International Space Station. During their 11-day mission aboard shuttle Atlantis, crew members delivered equipment, supplies and spare parts to the ISS. Following their mission report, astronauts visited with Stennis employees during a brief reception. Astronauts visiting Stennis were Pilot Barry Wilmore, Mission Specialist Randy Bresnik, Commander Charles Hobaugh and Mission Specialists Mike Foreman and Robert Satcher.

  18. Atmospheric Environment for Space Shuttle (STS-28) Launch

    Science.gov (United States)

    Jasper, G. L.; Batts, G. W.

    1990-01-01

    A summary is presented of selected atmospheric conditions observed near Space Shuttle STS-28 launch time on August 8, 1989. STS-28 carried a Department of Defense payload and the flight azimuth is denoted by a reference flight azimuth, since the actual flight azimuth is not known. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of prelaunch Jimsphere-measured vertical wind profiles is given. The final atmospheric tape, which consists of wind and thermodynamic parameters versus altitude, for STS-28 vehicle ascent was constructed and represents the best estimate of the launch environment to 400,000 ft altitude that was traversed by the STS-28 vehicle. The STS-28 ascent atmospheric data tape was constructed by Marshall Space Flight Center's Earth Science and Applications Division to provide an internally consistent data set for use in post-flight performance assessments.

  19. Atmospheric environment for space shuttle (STS-36) launch

    Science.gov (United States)

    Jasper, G. L.; Batts, G. W.

    1990-01-01

    A summary of selected atmospheric conditions observed near space shuttle STS-36 launch time on February 28, 1990, at Kennedy Space Center, Florida was presented. STS-36 carried a Department of Defense payload and the flight azimuth is not known. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of pre-launch Jimsphere-measured vertical wind profiles is given. The final atmospheric tape, which consists of wind and thermodynamic parameters versus altitude, for STS-36 vehicle ascent was constructed. The STS-36 ascent atmospheric data tape was constructed to provide an internally consistent data set for use in postflight performance assessments and represent the best estimate of the launch environment to the 400,000 feet altitude that was traversed by the STS-36 vehicle.

  20. Atmospheric environment for space shuttle (STS-33) launch

    Science.gov (United States)

    Jasper, G. L.; Batts, G. W.

    1990-01-01

    A summary is presented of selected atmospheric conditions observed near space shuttle STS-33 at launch time. STS-33 carried a DOD payload and the flight azimuth is denoted by a reference flight azimuth, since the actual flight azimuth is not known. Values of ambient pressure, temperature, moisture, ground winds, visual observations (clouds), and winds aloft are included. The sequence of pre-launch Jimsphere measured vertical wind profiles is given. The final atmospheric tape, which consists of wind and thermodynamic parameters versus altitude, for STS-33 vehicle ascent, was constructed. The STS-33 ascent atmospheric data tape was constructed by NASA-Marshall to provide an internally consistent data set for use in postflight performance assessments and represents the best estimates of the launch environment to the 400,000 ft altitude that was traversed by the STS-33 vehicle.

  1. Atmospheric environment for space shuttle (STS-38) launch

    Science.gov (United States)

    Jasper, G. L.; Batts, G. W.

    1991-01-01

    A summary of selected atmospheric conditions observed near space shuttle STS-38 launch time on November 15, 1990, at Kennedy Space Center is presented. STS-38 carried a Department of Defense payload and the flight azimuth in this report will be denoted by a reference flight azimuth, since the actual flight azimuth is not known. Values of ambient pressure, temperature, moisture, ground winds, visual observation (cloud), and winds aloft are included. The sequence of prelaunch Jimsphere-measured vertical wind profiles is presented. The final atmospheric tape, which consists of wind and thermodynamic parameters versus altitude, for STS-38 vehicle ascent was constructed. The STS-38 ascent atmospheric data tape was constructed by Marshall Space Flight Center's Earth Science and Applications Division to provide an internally consistent data set for use in postflight performance assessments and represents the best estimate of the launch environment to the 400,000-ft altitude that was traversed by the STS-38 vehicle.

  2. The impact of a STS/Constructivist learning approach on the beliefs and attitudes of preservice science teachers

    Science.gov (United States)

    Akcay, Hakan

    increased understanding of science processes and content. (4) An STS/Constructivist approach provides student--centered learning environments that are relevant, motivational, and meaningful for preservice science teachers. Further, it encourages them to interact and to participate more actively in science classrooms.

  3. STS-84 oxygen generator for Mir installation

    Science.gov (United States)

    1997-01-01

    McDonnell Douglas-SPACEHAB technicians strap in place a Russian- made oxygen generator on the floor of a SPACEHAB Double Module, being prepared for flight in the SPACEHAB Payload Processing Facility. From left, are Mark Halavin and Marc Tuttle. The oxygen generator, manufactured in Russia by RSC Energia, will be carried aboard the Space Shuttle Atlantis on Mission STS-84 for the Shuttles scheduled docking with the Russian Space Station Mir next month. The nearly 300-pound generator will replace one of two Mir units that have been malfunctioning recently. The generator functions by electrolysis, which separates water into its oxygen and hydrogen components. The hydrogen is vented and the oxygen is used for breathing by the Mir crew. The generator is 4.2 feet in length and 1.4 feet in diameter. STS-84, which is planned to include a Mir crew exchange of astronaut C. Michael Foale for Jerry M. Linenger, is targeted for a May 15 liftoff. It will be the sixth Shuttle-Mir docking.

  4. STS-41 Space Shuttle mission report

    Science.gov (United States)

    Camp, David W.; Germany, D. M.; Nicholson, Leonard S.

    1990-01-01

    The STS-41 Space Shuttle Program Mission Report contains a summary of the vehicle subsystem activities on this thirty-sixth flight of the Space Shuttle and the eleventh flight of the Orbiter vehicle, Discovery (OV-103). In addition to the Discovery vehicle, the flight vehicle consisted of an External Tank (ET) (designated as ET-39/LWT-32), three Space Shuttle main engines (SSME's) (serial numbers 2011, 2031, and 2107), and two Solid Rocket Boosters (SRB's), designated as BI-040. The primary objective of the STS-41 mission was to successfully deploy the Ulysses/inertial upper stage (IUS)/payload assist module (PAM-S) spacecraft. The secondary objectives were to perform all operations necessary to support the requirements of the Shuttle Backscatter Ultraviolet (SSBUV) Spectrometer, Solid Surface Combustion Experiment (SSCE), Space Life Sciences Training Program Chromosome and Plant Cell Division in Space (CHROMEX), Voice Command System (VCS), Physiological Systems Experiment (PSE), Radiation Monitoring Experiment - 3 (RME-3), Investigations into Polymer Membrane Processing (IPMP), Air Force Maui Optical Calibration Test (AMOS), and Intelsat Solar Array Coupon (ISAC) payloads. The sequence of events for this mission is shown in tabular form. Summarized are the significant problems that occurred in the Orbiter subsystems during the mission. The official problem tracking list is presented. In addition, each Orbiter problem is cited in the subsystem discussion.

  5. Atmospheric environment for space shuttle (STS-26) launch

    Science.gov (United States)

    Jasper, G. L.; Johnson, D. L.; Batts, G. W.

    1989-01-01

    A summary of selected atmospheric conditions observed near Space Shuttle STS-26 launch time on September 29, 1988, at Kennedy Space Center, Florida is given. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of pre-launch Jimsphere measured vertical wind profiles is given. The final atmospheric tape, which consists of wind and thermodynamic parameters versus altitude, for STS-26 vehicle ascent has been constructed. The STS-26 ascent atmospheric data tape has been constructed by Marshall Space Flight Center's Earth Science and Applications Division to provide an internally consistent data set for use in post-flight performance assessments.

  6. Atmospheric environment for space shuttle (STS-8) launch

    Science.gov (United States)

    Johnson, D. L.; Hill, C. K.; Turner, R. E.; Batts, G. W.

    1983-01-01

    Selected atmospheric conditions observed near Space Shuttle STS-8 launch time on August 30, 1983, at Kennedy Space Center, Florida are summarized. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of prelaunch Jimsphere measured vertical wind profiles is given. Also presented are wind and thermodynamic parameters representative of surface and aloft conditions in the SRB descent/impact ocean area. Final meteorological tapes, which consist of wind and thermodynamic parameters versus altitude, for STS-8 vehicle ascent and SRB descent/impact were constructed. The STS-8 ascent meteorological data tape was constructed.

  7. Atmospheric environment for Space Shuttle (STS-51A) launch

    Science.gov (United States)

    Johnson, D. L.; Jasper, G.; Hill, C. K.; Batts, G. W.

    1984-01-01

    Selected atmospheric conditions observed near Space Shuttle STS-51A launch time on November 8, 1984, are summarized. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of prelaunch Jimsphere measured vertical wind profiles is reported. The final atmospheric tape, which consists of wind and thermodynamic parameters versus altitude, for STS-51A vehicle ascent was constructed. The STS-51A ascent atmospheric data tape is constructed to provide an internally consistent data set for use in postflight performance assessments.

  8. Atmospheric environment for Space Shuttle (STS-51D)

    Science.gov (United States)

    Jasper, G. L.; Johnson, D. L.; Hill, C. K.; Batts, G. W.

    1985-01-01

    A summary of selected atmospheric conditions observed near the space shuttle STS-51D launch time on April 12, 1985, at Kennedy Space Center Florida is presented. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of prelaunch Jimsphere measured vertical wind profiles is given in this report. The final atmospheric tape, which consists of wind and thermodynamic parameters versus altitude, for STS-51D vehicle ascent is constructed. The STS-51D ascent atmospheric data tape is compiled by Marshall Space Flight Center's Atmospheric Sciences Division to provide an internally consistent data set for use in post-flight performance assessments.

  9. Atmospheric environment for space shuttle (STS-51L) launch

    Science.gov (United States)

    Jasper, G. L.; Johnson, D. L.; Alexander, M.; Fichtl, G. H.; Batts, G. W.

    1986-01-01

    A summary is given of selected atmospheric conditions observed near Space Shuttle STS-51L launch time on January 28, 1986, at Kennedy Space Center, Florida. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of pre-launch Jimsphere measured vertical wind profiles is given. The final atmospheric tape, which consists of wind and thermodynamic parameters versus altitude, for STS-51L vehicle ascent has been constructed. The STS-51L ascent atmospheric data tape has been constructed by Marshall Space Flight Center's Atmospheric Sciences Division to provide an internally consistent data set for use in post flight performance assessments.

  10. Atmospheric environment for space shuttle (STS-51C) launch

    Science.gov (United States)

    Jasper, G.; Johnson, D. L.; Hill, C. K.; Batts, G. W.

    1985-01-01

    Selected atmospheric conditions observed near space shuttle STS-51C launch time on January 24, 1985, at Kennedy Space Center, Florida are summarized. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of prelaunch Jimsphere measured vertical wind profiles are presented. The final atmospheric tape, which consists of wind and thermodynamic parameters versus altitude, for STS-51C vehicle ascent was constructed. The STS-51C ascent atmospheric data tape was constructed to provide an internally consistent data set for use in postflight performance assessments.

  11. Atmospheric environment for space shuttle (STS-51B) launch

    Science.gov (United States)

    Jasper, G. L.; Johnson, D. L.; Hill, C. K.; Batts, G. W.

    1985-01-01

    A summary of selected atmospheric conditions observed near space shuttle STS-51B launch time on April 29, 1985, at Kennedy Space Center Florida is presented. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of prelaunch Jimsphere measured vertical wind profiles is given. The final atmospheric tape, which consists of wind and thermodynamic parameters versus altitude, for STS-51B vehicle ascent was constructed. The STS-51B ascent atmospheric data tape was constructed by Marshall Space Flight Center's Atmospheric Sciences Division to provide an internally consistent data set for use in post flight performance assessments.

  12. Atmospheric environment for Space Shuttle (STS-9) launch

    Science.gov (United States)

    Johnson, D. L.; Hill, C. K.; Batts, G. W.

    1984-01-01

    This report presents a summary of selected atmospheric conditions observed near Space Shuttle STS-9 launch time on November 28, 1983, at Kennedy Space Center, Florida. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of prelaunch Jimsphere measured vertical wind profiles is given in this report. The final meteorological tape, which consists of wind and thermodynamic parameters versus altitude, for STS-9 vehicle ascent has been constructed. The STS-9 ascent meteorological data tape has been constructed by Marshall Space Flight Center in response to Shuttle task agreement No. 561-81-22-368 with Johnson Space Center.

  13. Atmospheric environment for Space Shuttle (STS-3) launch

    Science.gov (United States)

    Johnson, D. L.; Brown, S. C.; Batts, G. W.

    1982-01-01

    Selected atmospheric conditions observed near Space Shuttle STS-3 launch time on March 22, 1982, at Kennedy Space Center, Florida are summarized. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of prlaunch Jimsphere measured vertical wind profiles and the wind and thermodynamic parameters measured at the surface and aloft in the SRB descent/impact ocean area are presented. Final meteorological tapes, which consist of wind and thermodynamic parameters versus altitude, for STS-3 vehicle ascent and SRB descent were constructed. The STS-3 ascent meteorological data tape is constructed.

  14. Atmospheric environment for Space Shuttle (STS-2) launch

    Science.gov (United States)

    Johnson, D. L.; Brown, S. C.

    1981-01-01

    Selected atmospheric conditions observed near Space Shuttle STS-2 launch time on November 12, 1981, or Kennedy Space Center, Florida are summarized. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of prelaunch Jimsphere measured vertical wind profiles is given in this report. Wind and thermodynamic parameters measured at the surface and aloft in the SRB descent/impact ocean area are also presented. Final meteorological tapes, which consist of wind and thermodynamic parameters versus altitude, for STS-2 vehicle ascent and SRB descent have been constructed. The STS-2 ascent meteorological data tape was constructed.

  15. STS-69 flight day 6 highlights

    Science.gov (United States)

    1995-09-01

    After being awakened by the Beatles song, 'A Hard Days Night', the flightcrew of the STS-69 mission, Cmdr. Dave Walker, Pilot Ken Cockrell, and Mission Specialists Jim Voss, Jim Newman, and Mike Gernhardt, began their sixth day in orbit by monitoring the free orbiting Wake Shield Facility (WSF). Later Cmdr. Walker conducted an interview with television reporters from Atlanta and Boston, answering questions about the mission and general questions about NASA's space program. The crew filmed a video fo themselves performing daily routines (eating, shaving, exercising), as well as some of the physiological experiments, and shuttle equipment maintenance and checkout. One of the secondary experiments included the Commercial Generic Bioprocessing Apparatus-7 (CGBA-7), which served as an incubator and experiment station for a variety of tests (agricultural, pharmaceutical, biomedical, and environmental). Earth views included some cloud cover, the Gulf of Mexico, Texas, and the Atlantic Ocean.

  16. STS-110 Crew Interviews: Steve Smith

    Science.gov (United States)

    2002-01-01

    STS-110 Mission Specialist Steve Smith is seen during this preflight interview, where he gives a quick overview of the mission before answering questions about his inspiration to become an astronaut and his career path. Smith outlines his role in the mission in general, and specifically during the docking and extravehicular activities (EVAs). He describes the payload (S0 Truss and Mobile Transporter) and the dry run installation of the S0 truss that will take place the day before the EVA for the actual installation. Smith discusses the planned EVAs in detail and outlines what supplies will be left for the resident crew of the International Space Station (ISS). He ends with his thoughts on the most valuable aspect of the ISS.

  17. STS-110 Crew Interview: Jerry Ross

    Science.gov (United States)

    2002-01-01

    STS-110 Mission Specialist Jerry Ross is seen during this preflight interview, where he gives a quick overview of the mission before answering questions about his inspiration to become an astronaut and his career path. Ross outlines his role in the mission in general, and specifically during the docking and extravehicular activities (EVAs). He describes the payload (S0 Truss and Mobile Transporter) and the dry run installation of the S0 truss that will take place the day before the EVA for the actual installation. Ross discusses the planned EVAs in detail and outlines what supplies will be left for the resident crew of the International Space Station (ISS). He ends with his thoughts on the most valuable aspect of the ISS.

  18. STS-107 Flight Day 9 Highlights

    Science.gov (United States)

    2003-01-01

    This video shows the activities of the STS-107 crew (Rick Husband, Commander; William McCool, Pilot; Kalpana Chawla, David Brown, Michael Anderson, Laurel Clark, Mission Specialists; Ilan Ramon, Payload Specialist) during flight day 9 of the Columbia orbiter's final flight. The primary activities of flight day 9 are spaceborne experiments. The video shows a commercial experiment on roses, a partial view of Africa from Libya to the Horn of Africa through the MEIDEX (Mediterranean Israeli Dust Experiment), and the FAST (Facility for Absorption and Surface Tension) experiment. The STARS (Space Technology and Research Students) international student experiments are shown. The preliminary results of these experiments on the effects of microgravity on silkworms, spiders, crystal growth, fish embryos, carpenter bees, and ants are discussed. The video includes a view of southern Spain and the Mediterranean Sea.

  19. STS-69 flight day 9 highlights

    Science.gov (United States)

    1995-09-01

    The song, 'He's A Tramp', from the Walt Disney cartoon movie, 'Lady and the Tramp', awakened the astronauts, Cmdr. Dave Walker, Pilot Ken Cockrell, and Mission Specialists Jim Voss, Jim Newman, and Mike Gernhardt, on the ninth day of the STS-69 mission. The Wake Shield Facility (WSF) was again unberthed from the shuttle cargo bay and , using the shuttle's robot arm, held over the side of the shuttle for five hours where it collected data on the electrical field build-up around the spacecraft as part of the Charging Hazards and Wake Studies Experiment (CHAWS). Voss and Gernhardt rehearsed their Extravehicular Activity (EVA) spacewalk, which was planned for the next day. Earth views included cloud cover, a hurricane, and its eye.

  20. STS-97 Crew Interviews: Michael J. Bloomfield

    Science.gov (United States)

    2000-01-01

    Live footage of a preflight interview with Pilot Michael J. Bloomfield is shown. The interview addresses many different questions including why Bloomfield became interested in the space program, the events and people that influence him and ultimately led to his interest, and his vigorous training in the astronaut program. Other interesting information that this one-on-one interview discusses are the main goals of the STS-97 mission, its scheduled docking with the new International Space Station (ISS), and its delivery of the first set of U.S.-provided solar arrays, batteries, and radiators. Bloomfield briefly discusses his responsibilities during the much-anticipated docking as well as during the scheduled space-walks.

  1. STS-110 Crew Interview: Mike Bloomfield

    Science.gov (United States)

    2002-01-01

    STS-110 Commander Mike Bloomfield is seen during this preflight interview, where he gives a quick overview of the mission before answering questions about his inspiration to become an astronaut and his career path. Bloomfield outlines his role in the mission in general, and specifically during the docking and extravehicular activities (EVAs). He describes the payload (S0 Truss and Mobile Transporter) and the dry run installation of the S0 truss that will take place the day before the EVA for the actual installation. Bloomfield discusses the planned EVAs in detail and outlines what supplies will be left for the resident crew of the International Space Station (ISS). He ends with his thoughts on the most valuable aspect of the ISS.

  2. STS-114: Discovery Launch Readiness Press Conference

    Science.gov (United States)

    2005-01-01

    This press conference, attended by representatives from the national, Florida, and aerospace media, addresses launch, weather, and safety issues related to Space Shuttle Discovery prior to its launch on the STS-114 Return to Flight mission. The Master of Ceremonies is George Diller from NASA Public Affairs, and the panelists are: Space Shuttle Program Manager Bill Parsons, ISS Program Manager (JSC) Bill Gerstenmaier, Space Shuttle Deputy Program Manager Wayne Hale, Director of Shuttle Processing Mike Wetmore, ISS Program Manager (JAXA) Dr. Kuniaki Shiraki, and Launch Weather Officer (USAF) Mindy Chavez. Questions included the following topics: predicted weather conditions at launch, contingency rescue plans, countdown procedures, and risk management, as well as implications of the Return to Flight for the International Space Station (ISS).

  3. STS-114: Discovery Launch Postponement Press Briefing

    Science.gov (United States)

    2005-01-01

    This press briefing addresses the problem that occurred prior to the launch of the STS-114. Dean Acosta, Deputy Assistant Administrator of Public Affairs, introduces the panel which consists of Dr. Michael Griffin, NASA Administrator, William Readdy, Associate Administrator for Space Operations, Wayne Hale, Space Shuttle Program Deputy Manager, Steve Poulas, Orbiter Project Manager, Mike Leinbach, NASA Launch Director, and Bill Parsons, Space Shuttle Program Manager. Wayne Hale expresses that a problem occurred with one of the low level sensors in the hydrogen tank and that the cause of the problem must be identified and rectified. Steve Poulos talks about establishing a troubleshooting plan as a part of the scrub effort and Mike Leinbach describes the process of draining the external tank. Wayne Hale answers questions about the sensors and if the Space Shuttle Discovery is safe to fly and Steve Poulos answers questions about the possible suspects for this problem.

  4. STS-121 Extravehicular Activity (EVA) Imagery

    Science.gov (United States)

    2006-01-01

    Astronaut Michael E. Fossum, STS-121 mission specialist, used a digital still camera to expose a photo of his helmet visor during a session of extravehicular activity (EVA) while Space Shuttle Discovery was docked with the International Space Station (ISS). Also visible in the visor reflections are fellow space walker Piers J. Sellers, mission specialist, Earth's horizon, and a station solar array. During its 12-day mission, this utilization and logistics flight delivered a multipurpose logistics module (MPLM) to the ISS with several thousand pounds of new supplies and experiments. In addition, some new orbital replacement units (ORUs) were delivered and stowed externally on the ISS on a special pallet. These ORUs are spares for critical machinery located on the outside of the ISS. During this mission the crew also carried out testing of Shuttle inspection and repair hardware, as well as evaluated operational techniques and concepts for conducting on-orbit inspection and repair.

  5. Fluid Dynamic Verification Experiments on STS-70

    Science.gov (United States)

    Kleis, Stanley J.

    1996-01-01

    Fluid dynamic experiments were flown on STS-70 as phase two of the engineering evaluation of the first bioreactor Engineering Development Unit (EDU#1). The phase one experiments were comparative cell cultures in identical units on earth and onboard STS-70. In phase two, two types of fluid dynamic experiments were performed. Qualitative comparisons of the basic flow patterns were evaluated with the use of 'dye' streaklines formed from alternate injections of either a mild acid or base solution into the external flow loop that was then perfused into the vessel. The presence of Bromothymol Blue in the fluid then caused color changes from yellow to blue or vice versa, indicating the basic fluid motions. This reversible change could be repeated as desired. In the absence of significant density differences in the fluid, the flow patterns in space should be the same as on earth. Video tape records of the flow patterns for a wide range of operating conditions were obtained. The second type of fluid dynamic experiment was the quantitative evaluation of the trajectories of solid beads of various densities and sizes. The beads were introduced into the vessel and the paths recorded on video tape, with the vessel operated at various rotation rates and flow perfusion rates. Because of space limitations, the video camera was placed as close as possible to the vessel, resulting in significant optical distortion. This report describes the analysis methods to obtain comparisons between the in-flight fluid dynamics and numerical models of the flow field. The methods include optical corrections to the video images and calculation of the bead trajectories for given operating conditions and initial bead locations.

  6. Content Analysis

    National Research Council Canada - National Science Library

    Rossi, George Bedinelli; Serralvo, Francisco Antonio; João, Belmiro Nascimento

    2014-01-01

    This study introduces the various definitions and types of content analysis. This type of analysis historically presents itself as a quantitative approach to data analysis and currently shows up as a qualitative approach...

  7. Content Marketing

    OpenAIRE

    LE, DUC

    2013-01-01

    The purpose of this thesis was to introduce the new trend in today’s marketing world: content marketing. It has been employed by many companies and organizations in the world and has been proven success even when it is still a fairly new topic. Five carefully selected theories of content marketing proposed by experts in the field has been collected, compared and displayed as originally and scientifically as possible in this thesis. The chosen theories provide a diversified perspectives of...

  8. Atmospheric environment for space shuttle (STS-35) launch

    Science.gov (United States)

    Jasper, G. L.; Batts, G. W.

    1991-01-01

    A summary is given of selected atmospheric conditions observed near space shuttle STS-35 launch time on December 2, 1990, at Kennedy Space Center, Florida. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of prelaunch Jimsphere-measured vertical wind profiles is given in this report. The final atmospheric tape, which consists of wind and thermodynamic parameters versus altitude, for STS-35 vehicle ascent has been constructed. The STS-35 ascent atmospheric data tape has been constructed by Marshall Space Flight Center's Earth Science and Applications Division to provide an internally consistent data set for use in postflight performance assessments and represents the best estimate of the launch environment to the 400,000-ft altitude that was traversed by the STS-35 vehicle.

  9. Atmospheric environment for Space Shuttle (STS-37) launch

    Science.gov (United States)

    Jasper, G. L.; Batts, G. W.

    1991-01-01

    A summary of selected atmospheric conditions observed near Space Shuttle STS-37 launch time on 5 Apr. 1991 at KSC is presented. Values of ambient pressure, temperature, moisture, ground winds, visual observations (clouds), and winds aloft are included. The sequence of prelaunch Jimsphere-measured vertical wind profiles is given. The final atmospheric tape, which consists of wind and thermodynamic parameters versus altitude, for STS-37 vehicle ascent was constructed. The STS-37 ascent atmospheric data tape was constructed by Marshall Space Flight Center's Earth Science and Applications Division to provide an internally consistent data set for use in postflight performance assessments and represents the best estimate of the launch environment to the 400,000 ft. altitude that was traversed by the STS-37 vehicle.

  10. Atmospheric environment for Space Shuttle (STS-32) launch

    Science.gov (United States)

    Jasper, G. L.; Batts, G. W.

    1990-01-01

    A summary of selected atmospheric conditions observed near space shuttle STS-32 launch time on January 9, 1990, at Kennedy Space Center, Florida, are presented. Values of ambient pressure, temperature, moisture, ground winds, visual observations (clouds), and winds aloft are included. The sequence of pre-launch Jimsphere-measured vertical wind profiles is also presented. The final atmospheric tape, which consists of wind and thermodynamic parameters versus altitude, for STS-32 vehicle ascent was constructed. The STS-32 ascent atmospheric data tape was constructed to provide an internally consistent data set for use in postflight performance assessments and represents the best estimate of the launch environment that was traversed by the STS-32 vehicle.

  11. Atmospheric environment for Space Shuttle Atlantis (STS-43) launch

    Science.gov (United States)

    Jasper, G. L.; Batts, G. W.

    1992-01-01

    A summary of selected atmospheric conditions observed near Space Shuttle Atlantis (STS-43) launch time on August 2, 1991, at Kennedy Space Center, Florida is presented. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of prelaunch Jimsphere-measured vertical wind profiles is given. The final atmospheric profile, which consists of wind and thermodynamic parameters versus altitude, for STS-43 vehicle ascent was constructed. The STS-43 ascent atmospheric data profile was constructed by Marshall Space Flight Center's Earth Science and Applications Division to provide an internally consisted data set for use in postflight performance assessments and represents the best estimate of the launch environment to the 400,000-ft altitude that was traversed by the STS-43 vehicle.

  12. Atmospheric environment for Space Shuttle Atlantis (STS-39) launch

    Science.gov (United States)

    Jasper, G. L.; Batts, G. W.

    1992-01-01

    A summary is presented of selected atmospheric conditions observed near space shuttle Atlantis STS-39 launch time on 28 April 1991, at Kennedy Space Center, FL. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of prelaunch Jimsphere measured vertical wind profiles is given. The final atmospheric tape, which consists of wind and thermodynamic parameters versus altitude, for STS-39 vehicle ascent was constructed. The STS-39 ascent atmospheric data tape was constructed by NASA-Marshall to provide an internally consistent data set for use in postflight performance assessments and represents the best estimate of the launch environment to the 400,000 ft altitude that was traversed by the STS-39 vehicle.

  13. Atmospheric environment for Space Shuttle (STS-41G) launch

    Science.gov (United States)

    Johnson, D. L.; Hill, C. K.; Jasper, G.; Batts, G. W.

    1984-01-01

    Selected atmospheric conditions that were observed near Space Shuttle STS-41G launch time on October 5, 1984 at Kennedy Space Center in Florida are summarized. Values of ambient pressure, temperature, moisture, ground winds, visual obsrvations (cloud), and winds aloft are included. The sequence of prelaunch Jimsphere-measured vertical wind profiles is given. The final atmospheric tape consisting of wind and thermodynamic parameters versus altitude for STS-41G vehicle ascent was constructed. The STS-41G ascent atmospheric data tape was constructed. The STS-41G ascent atmospheric data tape was constructed by Marshall Space Flight Center's Atmospheric Sciences Division to provide an internally consistent data set for use in post flight performance assessments.

  14. Atmospheric environment for space shuttle (STS-41) launch

    Science.gov (United States)

    Jasper, G. L.; Batts, G. W.

    1990-01-01

    A summary of selected atmospheric conditions observed near space shuttle STS-41 launch time on October 6, 1990, at Kennedy Space Center, Florida are presented. Values of ambient pressure, temperature, moisture, ground winds, visual observations (clouds), and winds aloft are included. The sequence of prelaunch Jimsphere-measured vertical wind profiles is given. The final atmospheric tape, which consists of wind and thermodynamic parameters versus altitude, for STS-41 vehicle ascent was constructed. The STS-41 ascent atmospheric data tape was constructed by Marshall Space Flight Center's Earth Science and Applications Division to provide an internally consistent data set for use in postflight performance assessments and represents the best estimate of the launch environment to the 400,000 ft altitude that was traversed by the STS-41 vehicle.

  15. Atmospheric environment for Space Shuttle (STS-27) launch

    Science.gov (United States)

    Jasper, G. L.; Johnson, D. L.; Batts, G. W.

    1989-01-01

    Selected articles on atmospheric conditions observed near Space Shuttle STS-27 launch time on December 2, 1988, at Kennedy Space Center, Florida are summarized. STS-27 carried a Department of Defense payload and the flight azimuth in this report will be denoted by reference flight azimuth, since the actual flight azimuth is not known. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of pre-launch Jimsphere-measured vertical wind profiles is given. The final atmospheric tape, which consists of wind and thermodynamic parameters versus altitude, for STS-27 vehicle ascent was constructed. The STS-27 ascent atmospheric data tape was constructed by Marshall Space Flight Center's Earth Science and Applications Division to provide an internally consistent data set for use in post-flight performance assessments.

  16. Atmospheric environment for Space Shuttle Columbia (STS-40) launch

    Science.gov (United States)

    Jasper, G. L.; Batts, G. W.

    1992-01-01

    A summary of selected atmospheric conditions observed near the Space Shuttle Columbia (STS-40) launch time on 5 Jun. 1991, at KSC is presented. Values of ambient pressure, temperature, moisture, ground winds, visual observation (cloud), and winds aloft are included. The sequence of prelaunch Jimsphere-measured vertical wind profiles is given. The final atmospheric tape, which consists of wind and thermodynamic parameters versus altitude, for STS-40 vehicle ascent was constructed. The STS-40 ascent atmospheric data tape was constructed by MSFC's Earth Science and Applications Division to provide an internally consistent data set for use in postflight performance assessments and represents the best estimate of the launch environment to the 400,000-ft altitude that was traversed by the STS-40 vehicle.

  17. Atmospheric environment for Space Shuttle (STS-31) launch

    Science.gov (United States)

    Jasper, G. L.; Batts, G. W.

    1990-01-01

    A summary of selected atmospheric conditions observed near space shuttle STS-31 launch time on April 24, 1990, at Kennedy Space Center, Florida, are presented. Values of ambient pressure, temperature, moisture, ground winds, visual observations (clouds), and winds aloft are included. The sequence of pre-launch Jimsphere-measured vertical wind profiles is also presented. The final atmospheric tape, which consists if wind and thermodynamic parameters versus altitude, for STS-31 vehicle ascent was constructed. The STS-31 ascent atmospheric data tape was constructed to provide an internally consistent data set for use in postflight performance assessments and represent the best estimated of the launch environment to the 400,000 feet altitude that was traversed by the STS-31 vehicle.

  18. The development of STS payload environmental engineering standards

    Science.gov (United States)

    Bangs, W. F.

    1982-01-01

    The presently reported effort to provide a single set of standards for the design, analysis and testing of Space Transportation System (STS) payloads throughout the NASA organization must be viewed as essentially experimental, since the concept of incorporating the diverse opinions and experiences of several separate field research centers may in retrospect be judged too ambitious or perhaps even naive. While each STS payload may have unique characteristics, and the project should formulate its own criteria for environmental design, testing and evaluation, a reference source document providing coordinated standards is expected to minimize the duplication of effort and limit random divergence of practices among the various NASA payload programs. These standards would provide useful information to all potential STS users, and offer a degree of standardization to STS users outside the NASA organization.

  19. STS-65 Columbia, Orbiter Vehicle (OV) 102, crew insignia

    Science.gov (United States)

    1994-01-01

    STS-65 Columbia, Orbiter Vehicle (OV) 102, crew insignia (logo), the Official insignia of the NASA STS-65 International Microgravity Laboratory 2 (IML-2) mission. Designed by the crewmembers, the STS-65 insignia features the IML-2 mission and its Spacelab module which will fly aboard the Space Shuttle Columbia. IML-2 is reflected in the emblem by two gold stars shooting toward the heavens behind the IML lettering. The Space Shuttle Columbia is depicted orbiting the logo and reaching off into space, with Spacelab on an international quest for a better understanding of the effects of space flight on materials processing and life sciences. The STS-65 flight crewmembers are Commander Robert D. Cabana, Pilot James D. Halsell, Jr, Mission Specialist (MS) and Payload Commander (PLC) Richard J. Hieb, MS Carl E. Walz, MS Leroy Chiao, MS Donald A. Thomas, and Japanese Payload Specialist Chiaki Mukai.

  20. STS-9 commander John Young gets help adjusting headwear

    Science.gov (United States)

    1983-01-01

    Preparing for a training session is Astronaut John Young, commander of STS-9 Young gets help with his Snoopy cap or headwear housing communications gear, while one of his five fellow crewmembers, Dr. Ulf Merbold, looks on in the background.

  1. STS-107 Columbia sits inside an protective tent in VAB

    Science.gov (United States)

    2002-01-01

    KENNEDY SPACE CENTER, FLA. -- Columbia sits inside an protective tent used to keep out moisture. The orbiter is next scheduled to fly on mission STS-107 no earlier than Nov. 29. STS-107 is a research mission. The payload includes the Hitchhiker Bridge, a carrier for the Fast Reaction Experiments Enabling Science, Technology, Applications and Research (FREESTAR) that incorporates eight high priority secondary attached shuttle experiments, plus the SHI Research Double Module (SHI/RDM), also known as SPACEHAB.

  2. STS-73 Mission Specialist Catherine Coleman suits up

    Science.gov (United States)

    1995-01-01

    STS-73 Mission Specialist Catherine G. Coleman is assisted by a suit technician as she dons her launch/entry suit in the Operations and Checkout Building. STS-73 will be the first trip into space for Coleman, who will depart shortly for Launch Pad 39B, where the Space Shuttle Columbia awaits lift off during a window opening at 9:41 a.m. EDT, Oct. 7.

  3. Data on acute toxicity of the progestin STS 557.

    Science.gov (United States)

    Hillesheim, H G; Hoffmann, H

    1983-02-01

    In mice and rabbits of both sexes the acute toxicity of STS 557 (17 alpha-cyanomethyl-17 beta-hydroxy-estra-4, 9-dien-3-one) was determined after its oral or parenteral (i.p., s.c.) administration. In rabbits increasing lethality was observed following STS 557 suspended in tylose solution at the dose range of 1.0 to 3.0 g/kg p.o. or i.p. The approximate LD50-values were 1.0 to 1.5 g/kg for the i.p. route and 1.0 to 2.0 g/kg for the oral route. Levonorgestrel injected i.p. did not cause any lethality up to the dose of 3.0 g/kg. After oral or s.c. administration to mice, doses of 4.0 g/kg STS 557 were well tolerated. A dose-related toxicity occurred only after i.p. doses between 0.5 and 1.0 g/kg (STS 557), and between 2.0 and 4.0 g/kg (levonorgestrel), respectively. Using an oily vehicle for the oral route in mice, the lethal threshold dose for STS 557 was lowered to about 2.0 g/kg. In conclusion, a low oral acute toxicity was determined for STS 557 corresponding to that of other progestagens like levonorgestrel or norethisterone.

  4. Genome-Wide Profiling of Histone Modifications (H3K9me2 and H4K12ac) and Gene Expression in Rust (Uromyces appendiculatus) Inoculated Common Bean (Phaseolus vulgaris L.).

    Science.gov (United States)

    Ayyappan, Vasudevan; Kalavacharla, Venu; Thimmapuram, Jyothi; Bhide, Ketaki P; Sripathi, Venkateswara R; Smolinski, Tomasz G; Manoharan, Muthusamy; Thurston, Yaqoob; Todd, Antonette; Kingham, Bruce

    2015-01-01

    Histone modifications such as methylation and acetylation play a significant role in controlling gene expression in unstressed and stressed plants. Genome-wide analysis of such stress-responsive modifications and genes in non-model crops is limited. We report the genome-wide profiling of histone methylation (H3K9me2) and acetylation (H4K12ac) in common bean (Phaseolus vulgaris L.) under rust (Uromyces appendiculatus) stress using two high-throughput approaches, chromatin immunoprecipitation sequencing (ChIP-Seq) and RNA sequencing (RNA-Seq). ChIP-Seq analysis revealed 1,235 and 556 histone methylation and acetylation responsive genes from common bean leaves treated with the rust pathogen at 0, 12 and 84 hour-after-inoculation (hai), while RNA-Seq analysis identified 145 and 1,763 genes differentially expressed between mock-inoculated and inoculated plants. The combined ChIP-Seq and RNA-Seq analyses identified some key defense responsive genes (calmodulin, cytochrome p450, chitinase, DNA Pol II, and LRR) and transcription factors (WRKY, bZIP, MYB, HSFB3, GRAS, NAC, and NMRA) in bean-rust interaction. Differential methylation and acetylation affected a large proportion of stress-responsive genes including resistant (R) proteins, detoxifying enzymes, and genes involved in ion flux and cell death. The genes identified were functionally classified using Gene Ontology (GO) and EuKaryotic Orthologous Groups (KOGs). The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis identified a putative pathway with ten key genes involved in plant-pathogen interactions. This first report of an integrated analysis of histone modifications and gene expression involved in the bean-rust interaction as reported here provides a comprehensive resource for other epigenomic regulation studies in non-model species under stress.

  5. KDM4C, a H3K9me3 Histone Demethylase, is Involved in the Maintenance of Human ESCC-Initiating Cells by Epigenetically Enhancing SOX2 Expression

    Directory of Open Access Journals (Sweden)

    Xiang Yuan

    2016-10-01

    Full Text Available Our studies investigating the existence of tumor-initiating cell (TIC populations in human esophageal squamous cell carcinoma (ESCC had identified a subpopulation of cells isolated from ESCC patient-derived tumor specimens marked by an ALDHbri+ phenotype bear stem cell-like features. Importantly, KDM4C, a histone demethylase was enhanced in ALDHbri+ subpopulation, suggesting that strategies interfering with KDM4C may be able to target these putative TICs. In the present study, by genetic and chemical means, we demonstrated that, KDM4C blockade selectively decreased the ESCC ALDHbri+ TICs population in vitro and specifically targeted the TICs in ALDHbri+-derived xenograft, retarding engraftment. Subsequent studies of the KDM4C functional network identified a subset of pluripotency-associated genes (PAGs and aldehyde dehydrogenase family members to be preferentially down-regulated in KDM4C inhibited ALDHbri+ TICs. We further supported a model in which KDM4C maintains permissive histone modifications with a low level of H3K9 methylation at the promoters of several PAGs. Moreover, ectopic expression of SOX2 restored KDM4C inhibition-dependent ALDHbri+ TIC properties. We further confirmed these findings by showing that the cytoplasmic and nuclear KDM4C staining increased with adverse pathologic phenotypes and poor patient survival. Such staining pattern of intracellular KDM4C appeared to overlap with the expression of SOX2 and ALDH1. Collectively, our findings provided the insights into the development of novel therapeutic strategies based on the inhibition of KDM4C pathway for the eliminating of ESCC TIC compartment.

  6. STS-31 Space Shuttle mission report

    Science.gov (United States)

    Camp, David W.; Germany, D. M.; Nicholson, Leonard S.

    1990-01-01

    The STS-31 Space Shuttle Program Mission Report contains a summary of the vehicle subsystem activities on this thirty-fifth flight of the Space Shuttle and the tenth flight of the Orbiter Vehicle Discovery (OV-103). In addition to the Discovery vehicle, the flight vehicle consisted of an External Tank (ET) (designated as ET-34/LWT-27), three Space Shuttle main engines (SSME's) (serial numbers 2011, 2031, and 2107), and two Solid Rocket Booster (SRB) (designated as BI-037). The primary objective of the mission was to place the Hubble Space Telescope (HST) into a 330 nmi. circular orbit having an inclination of 28.45 degrees. The secondary objectives were to perform all operations necessary to support the requirements of the Protein Crystal Growth (PCG), Investigations into Polymer Membrane Processing (IPMP), Radiation Monitoring Equipment (RME), Ascent Particle Monitor (APM), IMAX Cargo Bay Camera (ICBC), Air Force Maui Optical Site Calibration Test (AMOS), IMAX Crew Compartment Camera, and Ion Arc payloads. In addition, 12 development test objectives (DTO's) and 10 detailed supplementary objectives (DSO's) were assigned to the flight. The sequence of events for this mission is shown. The significant problems that occurred in the Space Shuttle Orbiter subsystems during the mission are summarized, and the official problem tracking list is presented. In addition, each of the Space Shuttle Orbiter problems is cited in the subsystem discussion.

  7. STS-107 Flight Day 13 Highlights

    Science.gov (United States)

    2003-01-01

    This video shows the activities of the STS-107 crew on flight day 13 of the Columbia orbiter's final mission. The crew members include: Rick Husband, Commander; William McCool, Pilot; Kalpana Chawla, David Brown, Michael Anderson, Laurel Clark, Mission Specialists; Ilan Ramon, Payload Specialist. The primary activities of flight day 13 are spaceborne experiments, including troubleshooting undertaken by Mission Specialist Chawla on the Water Mist Fire Suppression (MIST) experiment. Chawla performs troubleshooting tasks relayed to her by Mission Control. She shows Mission Control the location of air and water in a transparent hose that is part of the atomizer on the exterior of the combustion module. She also changes the atomizer head. All six Space Technology and Research Students (STARS) experiments are profiled in the video. These experiments are on ants, crystal growth in a chemical garden, fish embryos, carpenter bees, spiders, and silkworms. The video also includes a view of the southeast Texas coast near Houston, and a view of Portugal, Spain, Gibraltar, Morocco, and the Sahara Desert. The video ends with an explanation of roses at Mission Control which commemorate astronauts who have died on missions.

  8. STS-52 Space Shuttle mission report

    Science.gov (United States)

    Fricke, Robert W., Jr.

    1992-12-01

    The STS-52 Space Shuttle Program Mission Report provides a summary of the Orbiter, External Tank (ET), Solid Rocket Booster/Redesigned Solid Rocket Motor (SRB/RSRM), and the Space Shuttle main engine (SSME) subsystem performance during the fifty-first flight of the Space Shuttle Program, and the thirteenth flight of the Orbiter vehicle Columbia (OV-102). In addition to the Orbiter, the flight vehicle consisted of the following: an ET (designated as ET-55/LWT-48); three SSME's, which were serial numbers 2030, 2015, and 2034 in positions 1, 2, and 3, respectively; and two SRB's, which were designated BI-054. The lightweight RSRM's that were installed in each SRB were designated 360L027A for the left SRB and 360Q027B for the right SRB. The primary objectives of this flight were to successfully deploy the Laser Geodynamic Satellite (LAGEOS-2) and to perform operations of the United States Microgravity Payload-1 (USMP-1). The secondary objectives of this flight were to perform the operations of the Attitude Sensor Package (ASP), the Canadian Experiments-2 (CANEX-2), the Crystals by Vapor Transport Experiment (CVTE), the Heat Pipe Performance Experiment (HPP), the Commercial Materials Dispersion Apparatus Instrumentation Technology Associates Experiments (CMIX), the Physiological System Experiment (PSE), the Commercial Protein Crystal Growth (CPCG-Block 2), the Shuttle Plume Impingement Experiment (SPIE), and the Tank Pressure Control Experiment (TPCE) payloads.

  9. STS-107 Flight Day 8 Highlights

    Science.gov (United States)

    2003-01-01

    This video shows the activities of the STS-107 crew (Rick Husband, Commander; William McCool, Pilot; Kalpana Chawla, David Brown, Michael Anderson, Laurel Clark, Mission Specialists, Ilan Ramon, Payload Specialist) during flight day 8 of the Columbia orbiter's final flight. The primary activities of flight day 8 are spaceborne experiments. Some background information is given on the SOFBALL (Structure of Flame Balls at Low Lewis-Number) microgravity experiment as footage of the flame balls is shown. The video also shows the MEIDEX (Mediterranean Israeli Dust Experiment) calibrating on the Moon. The six STARS (Space Technology and Research Students) international student experiments are profiled, including experiments on carpenter bees (Liechtenstein), spiders (Australia), silkworms (China), ants (United States), crystal growth (Israel), and fish embryos (Japan). A commercial experiment on roses is also profiled. Astronaut Clark gives a tour of the SpaceHab RDM (Research Double Module), in the space shuttle's payload bay. Astronauts McCool and Ramon take turns on an exercise machine. The video includes a partly cloudy view of the Pacific Ocean.

  10. Nonlinear Simulation Studies of Tokamaks and STs

    Energy Technology Data Exchange (ETDEWEB)

    W. Park; J. Breslau; J. Chen; G.Y. Fu; S.C. Jardin; S. Klasky; J. Menard; A. Pletzer; B.C. Stratton; D. Stutman; H.R. Strauss; L.E. Sugiyama

    2003-07-07

    The multilevel physics, massively parallel plasma simulation code, M3D, has been used to study spherical tori (STs) and tokamaks. The magnitude of outboard shift of density profiles relative to electron temperature profiles seen in NSTX [National Spherical Torus Experiment] under strong toroidal flow is explained. Internal reconnection events in ST discharges can be classified depending on the crash mechanism, just as in tokamak discharges; a sawtooth crash, disruption due to stochasticity, or high-beta disruption. Toroidal shear flow can reduce linear growth of internal kink. It has a strong stabilizing effect nonlinearly and causes mode saturation if its profile is maintained, e.g., through a fast momentum source. Normally, however, the flow profile itself flattens during the reconnection process, allowing a complete reconnection to occur. In some cases, the maximum density and pressure spontaneously occur inside the island and cause mode saturation. Gyrokinetic hot particle/MHD hybrid studies of NSTX show the effects of fluid compression on a fast-ion-driven n = 1 mode. MHD studies of recent tokamak experiments with a central current hole indicate that the current clamping is due to sawtooth-like crashes, but with n = 0.

  11. STS-47 Post Flight Press Conference

    Science.gov (United States)

    1992-01-01

    The flight crew of the STS-47 Space Shuttle Orbiter Endeavour Cmdr. Robert L. Gibson, Pilot Curtis L. Brown, Payload Cmdr. Mark C. Lee, Mission Specialists, N. Jan Davis, Jay Apt, Mae C. Jemison, and Payload Specialist, Mamoru Mohri, present an overview of their mission. This the 50th Shuttle flight marks the first NASA mission devoted primarily to Japan. Endeavour carries into Earth orbit Spacelab-J (SL-J), a 23-foot long pressurized laboratory built by the European Space Agency specifically for conducting experiments in a shirt-sleeve environment. SL-J contains 43 experiments, 34 provided by Japan, 7 from the United States and 2 joint experiments. Jemison becomes the first African American woman to fly in space and Mohri first Japanese to fly in space. Video footage includes the following: prelaunch and launch activities; various experiments including protein crystal growth, electronic materials, fluids, glasses and ceramics, metals and alloys, and the effects of microgravity on plants and animals; earth views of Japan, Tokyo Harbor, and Hurricane Bonnie; and the re-entry and landing of the orbiter.

  12. STS-68 Space Shuttle mission report

    Science.gov (United States)

    Fricke, Robert W., Jr.

    1995-01-01

    The STS-68 Space Shuttle Program Mission Report summarizes the Payload activities as well as the Orbiter, External Tank (ET), Solid Rocket Booster (SRB), Redesigned Solid Rocket Motor (RSRM), and the Space Shuttle main engine (SSME) systems performance during the sixty-fifth flight of the Space Shuttle Program and the seventh flight of the Orbiter vehicle Endeavour (OV-105). In addition to the Orbiter, the flight vehicle consisted of an ET that was designated ET-65; three SSMEs that were designated as serial numbers 2028, 2033, and 2026 in positions 1, 2, and 3, respectively; and two SRBs that were designated BI-067. The RSRMs that were installed in each SRB were designated as 360W040A for the left SRB and 360W040B for the right SRB. The primary objective of this flight was to successfully perform the operations of the Space Radar Laboratory-2 (SRL-2). The secondary objectives of the flight were to perform the operations of the Chromosome and Plant Cell Division in Space (CHROMEX), the Commercial Protein Crystal Growth (CPCG), the Biological Research in Canisters (BRIC), the Cosmic Radiation Effects and Activation Monitor (CREAM), the Military Application of Ship Tracks (MAST), and five Get-Away Special (GAS) payloads.

  13. STS-114 Flight Day 6 Highlights

    Science.gov (United States)

    2005-01-01

    Day 6 is a relatively quiet day for the STS-114 crew. The main responsibility for crew members of Space Shuttle Discovery (Commander Eileen Collins, Pilot James Kelly, Mission Specialists Soichi Noguchi, Stephen Robinson, Andrew Thomas, Wendy Lawrence, and Charles Camarda) and the Expedition 11 crew of the International Space Station (ISS) (Commander Sergei Krikalev and NASA ISS Science Officer and Flight Engineer John Phillips) is to unload supplies from the shuttle payload bay and from the Raffaello Multipurpose Logistics Module onto the ISS. Several of the astronauts answer interview questions from the news media, with an emphasis on the significance of their mission for the Return to Flight, shuttle damage and repair, and the future of the shuttle program. Thomas announces the winners of an essay contest for Australian students about the importance of science and mathematics education. The video includes the installation of a stowage rack for the Human Research Facility onboard the ISS, a brief description of the ISS modules, and an inverted view of the Nile Delta.

  14. STS-41-D Crew Enjoying Space

    Science.gov (United States)

    1984-01-01

    Crewmembers of NASA's 41-D mission take a group shot displaying their fun moments in space aboard the orbiter Discovery. Crewmembers are (counter-clockwise from center) crew commander Henry W. Harsfield Jr., pilot Michael L. Coats, mission specialist Steven A. Hawley, mission specialist Judith A. Resnik, payload specialist Charles D. Walker, and mission specialist Richard M. Mullane. Dr. Judith Resnik is shown enjoying the weightlessness of space during her first mission. Born on April 5, 1949 in Akron, Ohio, she received a Bachelor of Science degree in Electrical Engineering from Carnegie-Mellon University in 1970, and a Doctorate in Electrical Engineering from University of Maryland in 1977. Dr. Resnik joined NASA in 1978 as a senior systems engineer in product development with Xerox Corporation at El Segundo, California. NASA later selected her as an astronaut candidate in January 1978; she completed a 1-year training and evaluation period in August 1979. Dr. Resnik died on January 28, 1986 on her second mission, during the failed launch of Challenger STS-51 L.

  15. STS-114: Post Launch MMT Briefing

    Science.gov (United States)

    2005-01-01

    Bill Parsons, Space Shuttle Program Manager, and Wayne Hill, Deputy Manager of the Space Shuttle Program, and Chair of the Mission Management Team talks about the flight day 2 of the Discovery. Bill noted that flight operations are extremely going on well. He also explained an unexpected debris event on a power ramp little ways down where LH2 ramp begins. Before flight and based from technical data, slight modifications were done on the power ramp to ensure safety of return to flight. Bill also noted that STS-114 is a test flight; all data collected and brought back by the crew will be analyzed to ensure that all information needed is sufficient to work on faults and defects and to make appropriate repairs. Wayne discussed on flight safety. He emphasized the need to thoroughly inspect the thermal protection system of the Orbiter to ensure safe entry. Inspection of still photographs from the ISS and the boom sensor system scan are the primary means to understand engineering data in terms of the immediate flight safety. He also reported accomplishments for the day such as survey of the Orbiter boom system, all the wing leading edge, RCC panels and the nose cap, to make sure these are in good shape and working well, and re-emphasized that these are primary methods to clear the thermal protection system of the Orbiter to prove that it is safe to come home on this flight.

  16. Evaluation of Installation Methods for STS-2 Seismometers

    Science.gov (United States)

    Widmer-Schnidrig, R.; Kurrle, D.; Wielandt, E.

    2007-12-01

    In the course of the upgrade of the Gräfenberg Array (GRF) from Streckeisen STS-1 seismometers with 20 sec free period to STS-2 seismometers with 120 sec free period the question of how best to install the sensors was reconsidered. It was understood early on that the Streckeisen STS-1 seismometers need elaborate shielding in order to reach their full potential. Because some of the experience gained with the shielding of the STS-1 entered the design of the casing of the STS-2, it was not clear what kind of additional shielding was needed for getting best results with the STS-2. Since the first deployments of STS-2s starting with the German Regional Seismic Network (GRSN) in 1991, different types of insulations have been tried and it became apparent that the data quality at low frequencies can be markedly improved by extensive shielding of the sensors. In contrast to the STS-1 the STS-2 has a sealed casing such that variable air pressure should not lead to any buoyancy forces on the sensor masses. However, since the warp free design of the sensor does not completely remove pressure induced tilt, Wielandt proposed to install the STS-2 in a sealed container consisting of a thick gabbro base plate and an upside down stainless steel pot as cover (Stuttgart shielding). The German Regional Seismic Network (GRSN) and the Gräfenberg Array (GRF) are so far the only networks which have adopted this shielding. Its principal benefits are three fold: it reduces pressure fluctuations by approximately a factor of 30, reduces temperature fluctuations and keeps the sensor dry. We inspect two types of signals to make inferences about the STS-2 shielding used by different networks: Horizontal free mode spectra from the 2004 Sumatra earthquake and vertical component noise spectra tuned for the detection of the permanently excited background free oscillations (hum). The networks considered here are the GRSN, the GEOFON network of the GFZ Potsdam, the Swiss SDSnet and the Japanese F

  17. STS-74 Space Shuttle Mission Report

    Science.gov (United States)

    Fricke, Robert W., Jr.

    1996-01-01

    The STS-74 Space Shuttle Program Mission Report summarizes the Payload activities as well as the Orbiter, External Tank (ET), Solid Rocket Booster (SRB), Reusable Solid Rocket Motor (RSRM), and the Space Shuttle main engine (SSME) systems performance during the seventy-third flight of the Space Shuttle Program, the forty-eighth flight since the return-to-flight, and the fifteenth flight of the Orbiter Atlantis (OV-104). In addition to the Orbiter, the flight vehicle consisted of an ET that was designated ET-74; three Phase 11 SSME's that were designated as serial numbers 2012, 2026, and 2032 in positions 1, 2, and 3, respectively; and two SRB's that were designated BI-076. The RSRM's, designated RSRM-51, were installed in each SRB and the individual RSRM's were designated as 360TO51 A for the left SRB, and 360TO51 B for the right SRB. The primary objectives of this flight were to rendezvous and dock with the Mir Space Station and perform life sciences investigations. The Russian Docking Module (DM) was berthed onto the Orbiter Docking System (ODS) using the Remote Manipulator System (RMS), and the Orbiter docked to the Mir with the DM. When separating from the Mir, the Orbiter undocked, leaving the DM attached to the Mir. The two solar arrays, mounted on the DM, were delivered for future Russian installation to the Mir. The secondary objectives of the flight were to perform the operations necessary to fulfill the requirements of the GLO experiment (GLO-4)/Photogrammetric Appendage Structural Dynamics Experiment Payload (PASDE) (GPP), the IMAX Cargo Bay Camera (ICBC), and the Shuttle Amateur Radio Experiment-2 (SAREX-2). Appendix A lists the sources of data, both formal and informal, that were used to prepare this report. Appendix B provides the definition of acronyms and abbreviations used throughout the report. All times during the flight are given in Greenwich mean time (GMT)) and mission elapsed time (MET).

  18. STS-46 Space Shuttle mission report

    Science.gov (United States)

    Fricke, Robert W.

    1992-10-01

    The STS-46 Space Shuttle Program Mission Report contains a summary of the Orbiter, External Tank (ET), Solid Rocket Booster/Redesigned Solid Rocket Motor (SRB/RSRM), and the Space Shuttle main engine (SSME) subsystem performance during the forty-ninth flight of the Space Shuttle Program, and the twelfth flight of the Orbiter vehicle Atlantis (OV-104). In addition to the Atlantis vehicle, the flight vehicle consisted of the following: an ET, designated ET-48 (LWT-41); three SSME's, which were serial numbers 2032, 2033, and 2027 in positions 1, 2, and 3, respectively; and two SRB's which were designated BI-052. The lightweight/redesigned SRM's that were installed in each SRB were designated 360W025A for the left RSRM and 360L025B for the right RSRM. The primary objective of this flight was to successfully deploy the European Retrievable Carrier (EURECA) payload and perform the operations of the Tethered Satellite System-1 (TSS-1) and the Evaluation of Oxygen Interaction with Material 3/Thermal Energy Management Processes 2A-3 (EOIM-3/TEMP 2A-3). The secondary objectives of this flight were to perform the operations of the IMAX Cargo Bay Camera (ICBC), Consortium for Material Development in Space Complex Autonomous Payload-2 and 3 (CONCAP-2 and CONCAP-3), Limited Duration Space Environment Candidate Materials Exposure (LDCE), Pituitary Growth Hormone Cell Function (PHCF), and Ultraviolet Plume Instrumentation (UVPI). In addition to summarizing subsystem performance, this report also discusses each Orbiter, ET, SSME, SRB, and RSRM in-flight anomaly in the applicable section of the report. Also included in the discussion is a reference to the assigned tracking number as published on the Problem Tracking List. All times are given in Greenwich mean time (G.m.t.) as well as mission elapsed time (MET).

  19. STS-54 Space Shuttle mission report

    Science.gov (United States)

    Fricke, Robert W., Jr.

    1993-03-01

    The STS-54 Space Shuttle Program Mission Report is a summary of the Orbiter, External Tank (ET), Solid Rocket Booster/Redesigned Solid Rocket Motor (SRB/RSRM), and the Space Shuttle Main Engine (SSME) subsystems performance during this fifty-third flight of the Space Shuttle Program, and the third flight of the Orbiter vehicle Endeavour (OV-105). In addition to the Orbiter, the flight vehicle consisted of an ET, which was designated ET-51; three SSME's, which were serial numbers 2019, 2033, and 2018 in positions 1, 2, and 3, respectively; and two retrievable and reusable SRB's which were designated BI-056. The lightweight RSRM's that were installed in each SRB were designated 360L029A for the left SRB, and 360L029B for the right SRB. The primary objectives of this flight were to perform the operations to deploy the Tracking and Data Relay Satellite-F/Inertial Upper Stage payload and to fulfill the requirements of the Diffuse X-Ray Spectrometer (DXS) payload. The secondary objective was to fly the Chromosome and Plant Cell Division in Space (CHROMEX), Commercial Generic Bioprocessing Apparatus (CGBA), Physiological and Anatomical Rodent Experiment (PARE), and the Solid Surface Combustion Experiment (SSCE). In addition to presenting a summary of subsystem performance, this report also discusses each Orbiter, ET, SSME, SRB, and RSRM in-flight anomaly in the applicable section of the report. The official tracking number for each in-flight anomaly, assigned by the cognizant project, is also shown. All times are given in Greenwich mean time (G.m.t.) and mission elapsed time (MET).

  20. STS-112 Flight Day 4 Highlights

    Science.gov (United States)

    2002-10-01

    On the fourth day of STS-112, its crew (Jeffrey Ashby, Commander; Pamela Melroy, Pilot; David Wolf, Mission Specialist; Piers Sellers, Mission Specialist; Sandra Magnus, Mission Specialist; Fyodor Yurchikhin, Mission Specialist) onboard Atlantis and the Expedition 5 crew (Valery Korzun, Commander; Peggy Whitson, Flight Engineer; Sergei Treschev, Flight Engineer) onboard the International Space Station (ISS) are seen preparing for the installation of the S1 truss structure. Inside the Destiny Laboratory Module, Korzun and other crewmembers are seen as they busily prepare for the work of the day. Sellers dons an oxygen mask and uses an exercise machine in order to purge the nitrogen from his bloodstream, in preparation for an extravehicular activity (EVA). Whitson uses the ISS's Canadarm 2 robotic arm to grapple the S1 truss and remove it from Atlantis' payload bay, with the assistance of Magnus. Using the robotic arm, Whitson slowly maneuvers the 15 ton truss structure into alignment with its attachment point on the starboard side of the S0 truss structure, where the carefully orchestrated mating procedures take place. There is video footage of the entire truss being rotated and positioned by the arm, and ammonia tank assembly on the structure is visible, with Earth in the background. Following the completion of the second stage capture, the robotic arm is ungrappled from truss. Sellers and Wolf are shown exiting the the Quest airlock hatch to begin their EVA. They are shown performing a variety of tasks on the now attached S1 truss structure, including work on the Crew Equipment Translation Cart (CETA), the S-band Antenna Assembly, and umbilical cables that provide power and remote operation capability to cameras. During their EVA, they are shown using a foot platform on the robotic arm. Significant portions of their activities are shown from the vantage of helmet mounted video cameras. The video closes with a final shot of the ISS and its new S1 truss.

  1. MAIN CONTENTS

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Discussion on the Modern Agriculture Management Mode and its Innovation in China Mode of agricultural management is organic unify between form of agriculture management and way of agriculture management. Changes of agricultural management form are the content of system innovation;Transformation of agricultural management way belongs to the category of technical progress. We found that, precision agriculture with the moderate scale by professional farmers is the dominant mode of agricultural management. Coexisting of multiple modes is the pattern and evolution trend of modern agriculture management mode. The precision agriculture based on household business of moderate scale mode, we needs of rural economd socialization of

  2. Content Analysis

    Directory of Open Access Journals (Sweden)

    George Bedinelli Rossi

    2014-09-01

    Full Text Available This study introduces the various definitions and types of content analysis. This type of analysis historically presents itself as a quantitative approach to data analysis and currently shows up as a qualitative approach. The most common types are the conceptual and relational analysis. The latter receives influences of linguistic, cognitive and mental models and it is subdivided in affective extraction, analysis of proximity and cognitive mapping. Regarding the importance of this type of analysis, we have quantitative and qualitative character and the latter approach can be used to identify hypotheses, theoretical constructs or even models that can be tested by multivariate statistical techniques or even by experiments. 

  3. STS-92 - Landing at Edwards Air Force Base

    Science.gov (United States)

    2000-01-01

    The Space Shuttle Discovery glides in for landing at Edwards Air Force Base in Southern California at the conclusion of mission STS-92 on October 24, 2000. STS-92 was the 100th mission since the fleet of four Space Shuttles began flying in 1981. (Due to schedule changes, missions are not always launched in the order that was originally planned.) The almost 13-day mission was the last construction mission for the International Space Station prior to the first scientists taking up residency in the orbiting space laboratory the following month. The seven-member crew on STS-92 included mission specialists Koichi Wakata, Michael Lopez-Alegria, Jeff Wisoff, Bill McArthur and Leroy Chiao, pilot Pam Melroy and mission commander Brian Duffy.

  4. STS-93 Pilot Ashby suits up for launch

    Science.gov (United States)

    1999-01-01

    In the Operations and Checkout Building during final launch preparations for the third time, STS-93 Pilot Jeffrey S. Ashby pulls on his glove, part of his launch and entry suit. After Space Shuttle Columbia's July 20 and 22 launch attempts were scrubbed, the launch was again rescheduled for Friday, July 23, at 12:24 a.m. EDT. STS-93 is a five-day mission primarily to release the Chandra X-ray Observatory, which will allow scientists from around the world to study some of the most distant, powerful and dynamic objects in the universe. The STS-93 crew numbers five: Commander Eileen Collins, Ashby, and Mission Specialists Stephen A. Hawley (Ph.D.), Catherine G. Coleman (Ph.D.) and Michel Tognini of France, with the Centre National d'Etudes Spatiales (CNES). Collins is the first woman to serve as commander of a shuttle mission.

  5. STS-93 Pilot Ashby arrives at SLF for launch

    Science.gov (United States)

    1999-01-01

    STS-93 Pilot Jeffrey S. Ashby lands at Kennedy Space Center's Shuttle Landing Facility (SLF) aboard a T-38 jet aircraft. He and other crew members Commander Eileen Collins and Mission Specialists Steven A. Hawley (Ph.D.), Catherine G. 'Cady' Coleman (Ph.D.) and Michel Tognini of France, with the Centre National d'Etudes Spatiales (CNES), are arriving for pre-launch activities. STS-93 is Ashby's inaugural Shuttle flight. The primary mission of STS-93 is the release of the Chandra X-ray Observatory, which will allow scientists from around the world to study some of the most distant, powerful and dynamic objects in the universe. The new telescope is 20 to 50 times more sensitive than any previous X-ray telescope and is expected to unlock the secrets of supernovae, quasars and black holes.

  6. Atmospheric environment for Space Shuttle (STS-5) launch

    Science.gov (United States)

    Johnson, D. L.; Hill, C. K.; Batts, G. W.

    1983-01-01

    This report presents a summary of selected atmospheric conditions observed near Space Shuttle STS-5 launch time on November 11, 1982, at Kennedy Space Center, Florida. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of prelaunch Jimsphere measured vertical wind profiles is given in this report. Also presented are the wind and thermodynamic parameters measured at the surface and aloft in he SRB descent/impact ocean area. Final meteorological tapes, which consist of wind and thermodynamic parameters versus altitude, for STS-5 vehicle ascent and SRB descent have been constructed. The STS-5 ascent meteorological data tape has been constructed by Marshall Space Flight Center in response to Shuttle task agreement No. 936-53-22-368 with Johnson Space Center.

  7. Atmospheric environment for Space Shuttle (STS-41D) launch

    Science.gov (United States)

    Johnson, D. L.; Hill, C. K.; Jasper, G.; Batts, G. W.

    1984-01-01

    Selected atmospheric conditions observed near Space Shuttle STS-41D launch time on August 30, 1984, at Kennedy Space Center, Florida are summarized. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of prelaunch Jimsphere measured vertical wind profiles is given as well as wind and thermodynamic parameters representative of surface and aloft conditions in the SRB descent/impact ocean area. Final atmospheric tapes, which consist of wind and thermodynamic parameters versus altitude, for STS-41D vehicle ascent and SRB descent/impact were constructed. The STS-41D ascent meteorological data tape was constructed by Marshall Space Flight Center's Atmospheric Science Division to provide an internally consistent data set for use in post flight performance assessments.

  8. Atmospheric environment for space shuttle (STS-30) launch

    Science.gov (United States)

    Jasper, G. L.; Batts, G. W.

    1989-01-01

    This report presents a summary of selected atmospheric conditions observed near Space Shuttle STS-30 launch time on May 4, 1989, at Kennedy Space Center, Florida. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of pre-launch Jimsphere-measured vertical wind profiles is given in this report. The final atmospheric tape, which consists of wind and thermodynamic parameters versus altitude, for STS-30 vehicle ascent has been constructed. The STS-30 ascent atmospheric data tape has been constructed by Marshall Space Flight Center's Earth Science and Applications Division to provide an internally consistent data set for use in post-flight performance assessments.

  9. Atmospheric environment for Space Shuttle (STS-7) launch

    Science.gov (United States)

    Johnson, D. L.; Hill, C. K.; Batts, G. W.

    1983-01-01

    Selected atmospheric conditions observed near Space Shuttle STS-7 launch time on June 18, 1983, at Kennedy Space Center, Florida are summarized. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of prelaunch Jimsphere measured vertical wind profiles is given in this report. Also presented are wind and thermodynamic parameters representative of surface and aloft conditions in the SRB descent/impact ocean area. Final meteorological tapes, which consist of wind and thermodynamic parameters versus altitude, for STS-7 vehicle ascent and Acoustic/SRB descent have been constructed. The STS-7 ascent meteorological data tape has been constructed by Marshall Space Flight Center in response to Shuttle task agreement No. 936-53-22-368 with Johnson Space Center.

  10. Atmospheric environment for space shuttle (STS-34) launch

    Science.gov (United States)

    Jasper, G. L.; Batts, G. W.

    1989-01-01

    A summary of selected atmospheric conditions observed near space shuttle STS-34 launch time on October 18, 1989, at Kennedy Space Center, Florida is presented. Values of ambient pressure, temperature, moisture, ground winds, visual observations (clouds), and winds aloft are included. The sequence of pre-launch Jimsphere-measured vertical wind profiles is given. The final atmospheric tape, which consists of wind and thermodynamic parameters vs. altitude, for STS-34 vehicle ascent was constructed to provide an internally consistent data set for use in post-flight performance assessments. It represents the best estimates of the launch environment to the 400,000 feet altitude that was traversed by the STS-34 vehicle.

  11. Atmospheric environment for Space Shuttle (STS-6) launch

    Science.gov (United States)

    Johnson, D. L.; Hill, C. K.; Batts, G. W.

    1983-01-01

    Selected atmospheric conditions observed near Space Shuttle STS-6 launch time on April 4, 1983, at Kennedy Space Center, Florida are summarized. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of prelaunch Jimsphere measured vertical wind profiles is given. Also presented are the wind and thermodynamic parameters measured at the surface and aloft in the SRB descent/impact ocean area. Final meteorological tapes, which consist of wind and thermodynamic parameters versus altitude, for STS-6 veicle ascent and SRB descent were constructed. The STS-6 ascent meteorological data tape was constructed by Marshall Space Flight Center in response to Shuttle task agreement No. 936-53-22-368 with Johnson Space Center.

  12. Atmospheric environment for space shuttle (STS-29) launch

    Science.gov (United States)

    Jasper, G. L.; Batts, G. W.

    1989-01-01

    This report presents a summary of selected atmospheric conditions observed near Space Shuttle STS-29 launch time on March 13, 1989, at Kennedy Space Center, Florida. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of pre-launch Jimsphere-measured vertical wind profiles is given in this report. The final atmospheric tape, which consists of wind and thermodynamic parameters versus altitude, for STS-29 vehicle ascent has been constructed. The STS-29 ascent atmospheric data tape has been constructed by Marshall Space Flight Center's Earth Science and Applications Division to provide an internally consistent data set for use in post-flight performance assessments.

  13. STS-97 Pilot Bloomfield arrives at KSC for TCDT

    Science.gov (United States)

    2000-01-01

    STS-97 Pilot Mike Bloomfield arrives at the Shuttle Landing Facility aboard a T-38 jet aircraft. He and the rest of the crew are at KSC to take part in Terminal Countdown Demonstration Test activities that include emergency egress training, familiarization with the payload, and a simulated launch countdown. The other crew members are Commander Brent Jett, and Mission Specialists Joe Tanner, Carlos Noriega and Marc Garneau, who is with the Canadian Space Agency. Mission STS-97is the sixth construction flight to the International Space Station. Its payload includes the P6 Integrated Truss Structure and a photovoltaic (PV) module, with giant solar arrays that will provide power to the Station. The mission includes two spacewalks to complete the solar array connections. STS-97 is scheduled to launch Nov. 30 at 10:05 p.m. EST.

  14. Histone H3 lysine 9 methylation is associated with the expression of hMLH1 and DNA methylation in gastric cancer cells%胃癌细胞系中组蛋白H3-K9甲基化与错配修复基因hMLH1表达及DNA甲基化的关系

    Institute of Scientific and Technical Information of China (English)

    孟春风; 朱新江; 彭过; 戴冬秋

    2008-01-01

    Objective To identify the association of his tone H3 lysine 9 (H3-K9) methylafion with DNA methylation and the expression of the mismatch repair gene hMLH1 in human gastric cancer cells. Methods Gastric cancer cells of the lines BGC-823 and MGC-803 were cultured and treated with 5- Aza-2'-deoxycytidine (5-Aza-dC), a demethylation agent, for 72 hour. Chromatin immunoprecipitation (CHIP) assay was used to assess the status of histone H3 lysine 9 methylation in the promoter regions of hMLH1 gene. Methylation-specific PCR (MSP) was used to evaluate the effect of 5-Aza-dC on DNA methylation status. RT-PCR was used to examine the hMLH1 gene expression. Results In the MGC-803 cells, silenced hMLH1 gene was characterized by DNA methylation and histone H3-K9 hypermethylation; 5-Aza-dC demethylated the DNA and reduced the histone H3-K9 methylation at silenced loci and resulted in reactivation of hMLHI gene therein. Contrary to the MGC-803 ceils, BGC-823 cells expressed hMLH1 gene with DNA demethylation and histone H3-K9 hypomethylation; and 5-Aza-dC had no effects on the gene expression, DNA methylation, and histone H3-K9 methylation therein. Conclusion Hypermethylation of DNA in the promoter region is related to transcriptional silencing of hMLH1 gene. Histone H3-K9 methylation in different regions of the promoter studied correlates with DNA methylatian status of hMLH1 gene in gastric cancer cells. Alteration of DNA methylation affects histone H3-K9 methylation. 5-Aza-dC can control hMLHI expression, DNA methylation, and histone H3-K9 methylation in the promoter.%目的 探讨胃癌细胞系中组蛋白H3-K9甲基化与DNA甲基化及错配修复基因hMLHI表达的关系.方法 应用去甲基化制剂5-氮杂-2'-脱氧胞苷(5-Aza-dC)作用于2个胃癌细胞系,MGC-803和BGC-823.应用染色质免疫沉淀(CHIP)、甲基化特异性聚合酶链反应(MSP)和逆转录聚合酶链反应(RT-PCR)分析药物作用前后hMLH1基因启动子区域组蛋白H3-K9

  15. Characterization of Volume F Trash from the Three FY11 STS Missions: Trash Weights and Categorization and Microbial Characterization

    Science.gov (United States)

    Strayer, Richard F.; Hummerick, Mary E.; Richards, Jeffrey T.; McCoy, LaShelle E.; Roberts, Michael S.; Wheller, Raymond M.

    2011-01-01

    The project reported here provides microbial characterization support to the Waste Management Systems (WMS) element of NASA's Life Support and Habitation Systems (LSHS) program. Conventional microbiological methods were used to detect and enumerate microorganisms in STS Volume F Compartment trash for three shuttle missions: STS 133, 134, and 135. This trash was usually made available within 2 days of landing at KSC. The Volume F bag was weighed, opened and the contents were cataloged and placed into categories: personal hygiene items - inclUding EVA maximum absorbent garments (MAGs) and Elbow packs (daily toilet wipes, etc), drink containers, food waste (and containers), office waste (paper), and packaging materials - plastic film and duct tape. The average wet trash generation rate for the three STS missions was 0.362 % 0.157 kgwet crew 1 d-1 . This was considerably lower and more variable than the average rate for 4 STS missions reported for FY10. Trash subtotals by category: personal hygiene wastes, 56%; drink items, 11 %; food wastes, 18%; office waste, 3%; and plastic film, 12%. These wastes have an abundance of easily biodegraded compounds that can support the growth of microorganisms. Microbial characterization of trash showed that large numbers of bacteria and fungi have taken advantage of this readily available nutrient source to proliferate. Exterior and interior surfaces of plastic film bags containing trash were sampled and counts of cultivatable microbes were generally low and mostly occurred on trash bundles within the exterior trash bags. Personal hygiene wastes, drink containers, and food wastes and packaging all contained high levels of, mostly, aerobic heterotrophic bacteria and lower levels of yeasts and molds. Isolates from plate count media were obtained and identified .and were mostly aerobic heterotrophs with some facultative anaerobes. These are usually considered common environmental isolates on Earth. However, several pathogens were also

  16. STS-49 Lands at Edwards Air Force Base

    Science.gov (United States)

    1992-01-01

    STS-49 Orbiter Endeavour landed at Edwards Air Force Base on May 16, 1992 The drogue chute precedes the main chute in NASA's first exercise of its detailed test objective on the drag chute system. STS-49 ended its successful nine day mission dedicated to the retrieval, repair, and redeployment of the the INTELSAT VI (F-3) satellite. The communication satellite for the International Telecommunication Satellite organization had been stranded in an unusable orbit since its launch aboard the Titan rocket in March 1990. The mission marked the first time 3 astronauts worked simultaneously outside the space craft.

  17. STS-100 Pilot Ashby arrives at KSC before launch

    Science.gov (United States)

    2001-01-01

    KENNEDY SPACE CENTER, Fla. - STS-100 Pilot Jeffrey S. Ashby arrives at the KSC Shuttle Landing Facility aboard a T-38 jet aircraft to get ready for launch. The 11-day mission to the International Space Station will deliver and integrate the Spacelab Logistics Pallet/Launch Deployment Assembly, which includes the Space Station Remote Manipulator system and the UHF Antenna, and the Multi-Purpose Logistics Module Raffaello. Liftoff on mission STS-100 is scheduled at 2:41 p.m. EDT April 19.

  18. STS-112 Commander Ashby suits up for launch

    Science.gov (United States)

    2002-01-01

    KENNEDY SPACE CENTER, FLA. -- STS-112 Commander Jeffrey Ashby finishes suiting up for launch. STS-112 is the 15th assembly flight to the International Space Station, carrying the S1 Integrated Truss Structure and the Crew and Equipment Translation Aid (CETA) Cart A. The CETA is the first of two human-powered carts that will ride along the ISS railway, providing mobile work platforms for future spacewalking astronauts. On the 11-day mission, three spacewalks are planned to attach the S1 truss to the Station. Launch is scheduled for 3:46 p.m. EDT from Launch Pad 39B.

  19. STS-95 Pilot Steve Lindsey in white room

    Science.gov (United States)

    1998-01-01

    In the environmental chamber known as the white room, STS-95 Pilot Steven W. Lindsey is prepared by white room crew members Dave Law(left), Danny Wyatt and Travis Thompson (right) for entry into the Space Shuttle Discovery for his second flight into space. The STS-95 mission, targeted for launch at 2 p.m. EST on Oct. 29, is expected to last 8 days, 21 hours and 49 minutes, and return to KSC at 11:49 a.m. EST on Nov. 7.

  20. Atmospheric environment for Space Shuttle (STS-4) launch

    Science.gov (United States)

    Johnson, D. L.; Hill, C. K.; Batts, G. W.

    1982-01-01

    Selected atmospheric conditions observed near space shuttle STS-4 launch time on June 27, 1982, at Kennedy Space Center, Florida are summarized. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of prelaunch Jimsphere measured vertical wind profiles is given as well as the wind and thermodynamic parameters measured at the surface and aloft in the SRB descent/impact ocean area. Final meteorological tapes, which consist of wind descent were constructed. The STS-4 ascent meteorological data tape was constructed by Marshall Space Flight Center in response to shuttle task agreement No. 989-13-22-368 with Johnson Space Center.

  1. Atmospheric environment for Space Shuttle (STS-11) launch

    Science.gov (United States)

    Johnson, D. L.; Hill, C. K.; Batts, G. W.

    1984-01-01

    Atmospheric conditions observed near Space Shuttle STS-11 launch time on February 3, 1984, at Kennedy Space Center, Florida are summarized. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of prelaunch Jimsphere measured vertical wind profiles are reported. Wind and thermodynamic parameters representative of surface and aloft conditions in the SRB descent/impact ocean area are presented. Meteorological tapes, which consist of wind and thermodynamic parameters vesus altitude, for STS-11 vehicle ascent and SRB descent/impact were constructed.

  2. Atmospheric environment for space shuttle (STS-1) launch

    Science.gov (United States)

    Johnson, D. L.; Jasper, G.; Brown, S. C.

    1981-01-01

    Atmospheric conditions near space shuttle STS-1 launch time on April, 12, 1981, at Kennedy Space Center, Florida, are reported. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of prelaunch Jimsphere measured vertical wind profiles is presented. Also presented are the wind and thermodynamic parameters measured at the surface and aloft in the SRB descent/impact ocean area. Final meteorological data tapes for STS-1 vehicle ascent, and SRB descent have been constructed which consist of wind and thermodynamic parameters versus altitude.

  3. Atmospheric environment for space shuttle (STS-13) launch

    Science.gov (United States)

    Johnson, D. L.; Hill, C. K.; Jasper, G.; Batts, G. W.

    1984-01-01

    Selected atmospheric conditions observed near Space Shuttle STS-13 launch time on April 6, 1984, at Kennedy Space Center Florida are summarized. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), and winds aloft are included. The sequence of prelaunch Jimsphere measured vertical wind profiles is given. The final meteorological tape, which consists of wind and thermodynamic parameters versus altitude, for STS-13 vehicle ascent was constructed by Marshall Space Flight Center in response to shuttle task agreement No. 561-81-22-368 with Johnson Space Center.

  4. sY116, a human -linked polymorphic STS

    Indian Academy of Sciences (India)

    G. Mustafa Saifi; Reiner Veitia; Houssein Khodjet El Khil; Sandrine Barbaux; Preetha Tilak; I. Manorama Thomas; Marc Fellous

    2000-04-01

    During a study of deletions of Y-chromosomal DNA in infertile males, sY116, a Y-linked STS, showed different electrophoretic mobilities in three males, two infertile and one fertile. A study of this STS among 35 other normal males showed that this locus is polymorphic. sY116 has a poly A-rich stretch whose instability appears to be the most likely cause of this polymorphism. The possible usefulness of sY116 polymorphism in the detection of subtle genome-wide instabilities in some types of cancer is discussed.

  5. STS-based education in non-majors college biology

    Science.gov (United States)

    Henderson, Phyllis Lee

    The study explored the effect of the science-technology-society (STS) and traditional teaching methods in non-majors biology classes at a community college. It investigated the efficacy of the two methods in developing cognitive abilities at Bloom's first three levels of learning. It compared retention rates in classes taught in the two methods. Changes in student attitude relating to anxiety, fear, and interest in biology were explored. The effect of each method on grade attainment among men and women was investigated. The effect of each method on grade attainment among older and younger students was examined. Results of the study indicated that no significant differences, relating to retention or student attitude, existed in classes taught in the two methods. The study found no significant cognitive gains at Bloom's first three levels in classes taught in the traditional format. In the STS classes no significant gains were uncovered at Bloom's first level of cognition. Statistically significant gains were found in the STS classes at Bloom's second and third levels of cognition. In the classes taught in the traditional format no difference was identified in grade attainment between males and females. In the STS-based classes a small correlational difference between males and females was found with males receiving lower grades than expected. No difference in grade attainment was found between older and younger students taught in the traditional format. In the STS-based classes a small statistically significant difference in grade attainment was uncovered between older and younger students with older students receiving more A's and fewer C's than expected. This study found no difference in the grades of older, female students as compared to all other students in the traditionally taught classes. A weak statistically significant difference was discovered between grade attainment of older, female students and all other students in the STS classes with older, female

  6. STS-112 M.S. Magnus suits up before launch

    Science.gov (United States)

    2002-01-01

    KENNEDY SPACE CENTER, FLA. -- STS-112 Mission Specialist Sandra Magnus finishes suiting up before launch. STS-112 is the 15th assembly flight to the International Space Station, carrying the S1 Integrated Truss Structure and the Crew and Equipment Translation Aid (CETA) Cart A. The CETA is the first of two human-powered carts that will ride along the ISS railway, providing mobile work platforms for future spacewalking astronauts. On the 11-day mission, three spacewalks are planned to attach the S1 truss to the Station. Launch is scheduled for 3:46 p.m. EDT from Launch Pad 39B.

  7. STS-100 MS Phillips is fully suited up for launch

    Science.gov (United States)

    2001-01-01

    KENNEDY SPACE CENTER, Fla. - STS-100 Mission Specialist John L. Phillips is fully suited for launch. The 11-day mission to the International Space Station will deliver and integrate the Spacelab Logistics Pallet/Launch Deployment Assembly, which includes the Space Station Remote Manipulator system and the UHF Antenna, and the Multi-Purpose Logistics Module Raffaello. The mission includes two planned spacewalks for installation of the SSRMS. The mission is also the inaugural flight of Raffaello, carrying resupply stowage racks and resupply/return stowage platforms. Liftoff on mission STS-100 is scheduled at 2:41 p.m. EDT April 19.

  8. STS-100 MS Phillips arrives at KSC before launch

    Science.gov (United States)

    2001-01-01

    KENNEDY SPACE CENTER, Fla. - STS-100 Mission Specialist John L. Phillips arrives at the KSC Shuttle Landing Facility aboard a T- 38 jet aircraft to get ready for launch. The 11-day mission to the International Space Station will deliver and integrate the Spacelab Logistics Pallet/Launch Deployment Assembly, which includes the Space Station Remote Manipulator system and the UHF Antenna, and the Multi-Purpose Logistics Module Raffaello. Liftoff on mission STS-100 is scheduled at 2:41 p.m. EDT April 19.

  9. Post STS-135 Evaluation of Main Flame Deflector Witness Materials

    Science.gov (United States)

    Long, Victoria

    2011-01-01

    NASA and USA design engineers submitted witness materials from the solid rocket booster (SRB) main flame deflector for evaluation after the launch of STS-135. The following items were submitted for analysis: HY-80 steel witnes rods, 304 sta inles steel caps, and tungsten pistons. All of the items were photographed in order to document their condition after the launch of STS-135. The submitted samples were dimensionally measured in order to determine the amount of material lost during launch. Microstructural changes were observed in the HY-80 witness rod metallographic samples due to the heat of the launch.

  10. Post STS-134 Evaluation of Main Flame Deflector Witness Materials

    Science.gov (United States)

    Long, Victoria

    2011-01-01

    NASA and USA design engineers submitted witness materials from the solid rocket booster (SRB) main flame deflector for evaluation after the launch of STS-134. The following items were submitted for analysis: 1018 steel witness rods 304 stainless steel caps, tungsten pistons, and A-286 piston sleeves. All of the items were photographed in order to document their condition after the launch of STS-134. All of the items were dimensionally measured in order to determine the amount of material lost during launch. Microstructural changes were observed in the 1018 witness rod metallographic samples due to the heat of the launch

  11. STS-112 M.S. Sellers suits up for launch

    Science.gov (United States)

    2002-01-01

    KENNEDY SPACE CENTER, FLA. - During suitup for launch, STS-112 Mission Specialist Piers Sellers smiles in anticipation of his first Shuttle flight. STS-112 is the 15th assembly flight to the International Space Station, carrying the S1 Integrated Truss Structure and the Crew and Equipment Translation Aid (CETA) Cart A. The CETA is the first of two human-powered carts that will ride along the ISS railway, providing mobile work platforms for future spacewalking astronauts. On the 11-day mission, three spacewalks are planned to attach the S1 truss to the Station. Launch is scheduled for 3:46 p.m. EDT from Launch Pad 39B.

  12. STS-114: Discovery L-1 Countdown Status Briefing STS-114/Discovery L-2 Countdown Status Briefing

    Science.gov (United States)

    2005-01-01

    Bruce Buckingham from NASA Public Affairs introduces Jeff Spaulding, NASA Test Director and Kathy Winters, Shuttle Weather Officer in this L-2 countdown status briefing. Spaulding gives the Space Shuttle launch countdown status before lift-off on July 26th. He expresses that vehicle launch and ground systems are performing well and that there are no significant issues in preparation for the launch. The crew arrival time to the launching pad and the launch window for Discovery is also discussed. He ends his countdown status by expressing that the Discovery Orbiter is the safest Shuttle to date. Kathy Winters gives her weather forecast for the launch day. She presents a satellite picture of tropical storm Franklin and charts showing the STS-114 Tanking Forecast, Launch Forecast, Solid Rocket Booster (SRB) recovery, Continental United States (CONUS) launch, Transoceanic Abort Landing (TAL) launch, 24 and 48 Hour Delay, CONUS 24 and 48 hour delay, and TAL 24 and 48 hr delay. Questions from the news media about the mood of the test engineers as launch day is approached are answered. Jessica Rye from NASA Public Affairs introduces Pete Nikolento, NASA Test Director; Scott Higgenbotham, STS-114 Payload Mission Manager; and Kathy Winters, Space Shuttle Weather Officer in this L-1 Countdown Status Briefing. Nikolento expresses that the completion of the main engine system check-outs and servicing of on-board fuel-cell reactants have been completed. He also talks about pad closeouts and external cryogenic loads prior to launch. Scott Higgenbotham gives the payload status and Kathy Winters talks about her weather forecast for launch. Questions about the ecosensors, TAL sites, weather forecast and thoughts about return to flight are addressed.

  13. STS-29 Landing Approach at Edwards

    Science.gov (United States)

    1989-01-01

    The STS-29 Space Shuttle Discovery mission approaches for a landing at NASA's then Ames-Dryden Flight Research Facility, Edwards AFB, California, early Saturday morning, 18 March 1989. Touchdown was at 6:35:49 a.m. PST and wheel stop was at 6:36:40 a.m. on runway 22. Controllers chose the concrete runway for the landing in order to make tests of braking and nosewheel steering. The STS-29 mission was very successful, completing the launch a Tracking and Data Relay communications satellite, as well as a range of scientific experiments. Discovery's five man crew was led by Commander Michael L. Coats, and included pilot John E. Blaha and mission specialists James P. Bagian, Robert C. Springer, and James F. Buchli. Space Shuttles are the main element of America's Space Transportation System and are used for space research and other space applications. The shuttles are the first vehicles capable of being launched into space and returning to Earth on a routine basis. Space Shuttles are used as orbiting laboratories in which scientists and mission specialists conduct a wide variety of scientific experiments. Crews aboard shuttles place satellites in orbit, rendezvous with satellites to carry out repair missions and return them to space, and retrieve satellites and return them to Earth for refurbishment and reuse. Space Shuttles are true aerospace vehicles. They leave Earth and its atmosphere under rocket power provided by three liquid-propellant main engines with two solid-propellant boosters attached plus an external liquid-fuel tank. After their orbital missions, they streak back through the atmosphere and land like airplanes. The returning shuttles, however, land like gliders, without power and on runways. Other rockets can place heavy payloads into orbit, but, they can only be used once. Space Shuttles are designed to be continually reused. When Space Shuttles are used to transport complete scientific laboratories into space, the laboratories remain inside the payload

  14. Systematic Treatment Selection (STS): A Review and Future Directions

    Science.gov (United States)

    Nguyen, Tam T.; Bertoni, Matteo; Charvat, Mylea; Gheytanchi, Anahita; Beutler, Larry E.

    2007-01-01

    Systematic Treatment Selection (STS) is a form of technical eclectism that develops and plans treatments using empirically founded principles of psychotherapy. It is a model that provides systematic guidelines for the utilization of different psychotherapeutic strategies based on patient qualities and problem characteristics. Historically, it…

  15. STS-93 Pilot Ashby takes part in emergency egress training

    Science.gov (United States)

    1999-01-01

    STS-93 Pilot Jeffrey S. Ashby pauses for the photographer before climbing into an M-113 armored personnel carrier at the launch pad to take part in emergency egress training. In preparation for their mission, the STS-93 crew are participating in Terminal Countdown Demonstration Test activities that also include a launch-day dress rehearsal culminating with a simulated main engine cut-off. Others in the crew are Commander Eileen M. Collins and Mission Specialists Steven A. Hawley (Ph.D.), Catherine G. Coleman (Ph.D.), and Michel Tognini of France, who represents the Centre National d'Etudes Spatiales (CNES). Collins is the first woman to serve as a mission commander. The primary mission of STS-93 is the release of the Chandra X-ray Observatory, which will allow scientists from around the world to obtain unprecedented X-ray images of exotic environments in space to help understand the structure and evolution of the universe. Chandra is expected to provide unique and crucial information on the nature of objects ranging from comets in our solar system to quasars at the edge of the observable universe. Since X-rays are absorbed by the Earth's atmosphere, space-based observatories are necessary to study these phenomena and allow scientists to analyze some of the greatest mysteries of the universe. The targeted launch date for STS-93 is no earlier than July 20 at 12:36 a.m. EDT from Launch Pad 39B.

  16. Katz model prediction of Caenorhabditis elegans mutagenesis on STS-42

    Science.gov (United States)

    Cucinotta, Francis A.; Wilson, John W.; Katz, Robert; Badhwar, Gautam D.

    1992-01-01

    Response parameters that describe the production of recessive lethal mutations in C. elegans from ionizing radiation are obtained with the Katz track structure model. The authors used models of the space radiation environment and radiation transport to predict and discuss mutation rates for C. elegans on the IML-1 experiment aboard STS-42.

  17. Beyond the Triple Helix: Framing STS in the Developmental Context

    Science.gov (United States)

    Amir, Sulfikar; Nugroho, Yanuar

    2013-01-01

    For the past three decades or so, the field of Science and Technology Studies (STS) has shed light on the interrelationship between modern science and technology, on one side, and contemporary society, on the other. A majority of this knowledge and insights are situated in the context of Western societies, or more precisely, in economically and…

  18. STS-86 crew members Bloomfield and Chretien in white room

    Science.gov (United States)

    1997-01-01

    While a white room closeout crew member looks on, STS-86 Pilot Michael J. Bloomfield, at right, gets some assistance from fellow crew member, Mission Specialist Jean-Loup J.M. Chretien of the French Space Agency, CNES, before entering the Space Shuttle Atlantis at Launch Pad 39A.

  19. STS-112 M.S. Magnus suits up for launch

    Science.gov (United States)

    2002-01-01

    KENNEDY SPACE CENTER, FLA. --STS-112 Mission Specialist Sandra Magnus dons her space helmet for a final fit check in preparation for her launch to the International Space Station aboard Atlantis. Launch is scheduled for Oct. 2 between 2 and 6 p.m. EDT.

  20. STS-69 Crew members display 'Dog Crew' patches

    Science.gov (United States)

    1995-01-01

    Following their arrival at KSC's Shuttle Landing Facility, the five astronauts assigned to Space Shuttle Mission STS-69 display the unofficial crew patch for their upcoming spaceflight: the Dog Crew II patch. Mission Commander David M. Walker (center) and Payload Commander James S. Voss (second from right) previously flew together on Mission STS-53, the final dedicated Department of Defense flight on the Space Shuttle. A close comradery formed among Walker, Voss and the rest of the crew, and they dubbed themselves the 'dogs of war', with each of the STS-53 'Dog Crew' members assigned a 'dog tag' or nickname. When the STS-69 astronauts also became good buddies, they decided it was time for the Dog Crew II to be named. Walker's dog tag is Red Dog, Voss's is Dogface, Pilot Kenneth D. Cockrell (second from left) is Cujo, space rookie and Mission Specialist Michael L. Gernhardt (left) is Under Dog, and Mission Specialist James H. Newman (right) is Pluato. The Dog Crew II patch features a bulldog peering out from a doghouse shaped like the Space Shuttle and lists the five crew member's dog names. The five astronauts are scheduled to lift off on the fifth Shuttle flight of the year at 11:04 a.m. EDT, August 31, aboard the Space Shuttle Endeavour.

  1. STS-27 Atlantis, OV-104, solid rocket booster (SRB) inspection

    Science.gov (United States)

    1988-01-01

    Engineers, kneeling inside a hollow solid rocket booster (SRB), closely inspect the SRB segments and seams in the Kennedy Space Center (KSC) rotation and processing facility. The SRB will be used on STS-27 Atlantis, Orbiter Vehicle (OV) 104. The booster segments were transported via rail car from Morton Thiokol's Utah manufacturing plant. View provided by KSC with alternate number KSC-88PC-492.

  2. STS 41-D mission specialist Judith Resnik reivews headset interfac

    Science.gov (United States)

    1983-01-01

    STS 41-D mission specialist Judith Resnik and trainer review the headset interface units of the communication kit assemblies to be used during mission. Resnick is wearing the headset interface unit and headset and is examing the documentation attached to the equipment.

  3. STS-88 crew goes through Crew Equipment Interface Testing

    Science.gov (United States)

    1998-01-01

    -- In the Orbiter Processing Facility Bay 1, STS-88 Mission Specialists (left to right) Jerry L. Ross; Sergei Krikalev, a cosmonaut from Russia; and James H. Newman examine equipment that will be on the Space Shuttle Endeavour during their upcoming flight. Launch of Mission STS-88 is targeted for Dec. 3, 1998. The STS-88 crew members are participating in a Crew Equipment Interface Test (CEIT), familiarizing themselves with the orbiter's midbody and crew compartments. Other crew members are Commander Robert D. Cabana, Pilot Frederick W. 'Rick' Sturckow and Mission Specialist Nancy J. Currie. STS-88 will be the first Space Shuttle launch for assembly of the International Space Station (ISS). The primary payload is the Unity connecting module which will be mated to the Russian-built Zarya control module, expected to be already on orbit after a November launch from Russia. The first major U.S.-built component of ISS, Unity will serve as a connecting passageway to living and working areas of the space station. Unity has two attached pressurized mating adapters (PMAs) and one stowage rack installed inside. PMA-1 provides the permanent connection point between Unity and Zarya; PMA-2 will serve as a Space Shuttle docking port. Zarya is a self-supporting active vehicle, providing propulsive control capability and power during the early assembly stages. It also has fuel storage capability.

  4. Mission Operations Control Room Activities during STS-2 mission

    Science.gov (United States)

    1981-01-01

    Mission Operations Control Room (MOCR) activities during STS-2 mission. Overall view of the MOCR in the Johnson Space Center's Mission Control Center. At far right is Eugene F. Kranz, Deputy Director of Flight Operations. At the flight director console in front of Kranz's FOD console are Flight Directors M.P. Frank, Neil B. Hutchinson and Donald R. Puddy as well as others (39506); Wide-angle view of flight controllers in the MOCR. Clifford E. Charlesworth, JSC Deputy Director, huddles with several flight directors for STS-2 at the flight director console. Kranz, is at far right of frame (39507); Dr. Christopher C. Kraft, Jr., JSC Director, center, celebrates successful flight and landing of STS-2 with a cigar in the MOCR. He is flanked by Dr. Maxime A Faget, left, Director of Engineering and Development, and Thomas L. Moser, of the Structures and Mechanics Division (39508); Flight Director Donald R. Puddy, near right, holds replica of the STS-2 insignia. Insignias on the opposite wall

  5. L-Area STS MTR/NRU/NRX Grapple Assembly Closure Mechanics Review

    Energy Technology Data Exchange (ETDEWEB)

    Huizenga, D. J. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River Nuclear Solutions (SRNS)

    2016-06-08

    A review of the closure mechanics associated with the Shielded Transfer System (STS) MTR/NRU/NRX grapple assembly utilized at the Savannah River Site (SRS) was performed. This review was prompted by an operational event which occurred at the Canadian Nuclear Laboratories (CNL) utilizing a DTS-XL grapple assembly which is essentially identical to the STS MTR/NRU/NRX grapple assembly used at the SRS. The CNL operational event occurred when a NRU/NRX fuel basket containing spent nuclear fuel assemblies was inadvertently released by the DTS-XL grapple assembly during a transfer. The SM review of the STS MTR/NRU/NRX grapple assembly will examine the operational aspects of the STS and the engineered features of the STS which prevent such an event at the SRS. The design requirements for the STS NRU/NRX modifications and the overall layout of the STS are provided in other documents.

  6. Syntheses, structures, and spectroscopic properties of K9Nd[PS4]4, K3Nd[PS4]2, Cs3Nd[PS4]2, and K3Nd3[PS4]4.

    Science.gov (United States)

    Wu, Yuandong; Bensch, Wolfgang

    2008-09-01

    Four new quaternary alkali neodymium thiophosphates K 9Nd[PS 4] 4 ( 1), K 3Nd[PS 4] 2 ( 2), Cs 3Nd[PS 4] 2 ( 3), and K 3Nd 3[PS 4] 4 ( 4) were synthesized by reacting Nd with in situ formed fluxes of K 2S 3 or Cs 2S 3, P 2S 5 and S in appropriate molar ratios at 973 K. Their crystal structures are determined by single crystal X-ray diffraction. Crystal data: 1: space group C2/ c, a = 20.1894(16), b = 9.7679(5), c = 17.4930(15) A, beta = 115.66(1) degrees , and Z = 4; 2: space group P2 1/ c, a = 9.1799(7), b = 16.8797(12), c = 9.4828(7) A, beta = 90.20(1) degrees , and Z = 4; 3: space group P2 1/ n, a = 15.3641(13), b = 6.8865(4), c = 15.3902(13) A, beta = 99.19(1) degrees , and Z = 4; 4: space group C2/ c, a = 16.1496(14), b = 11.6357(7), c = 14.6784(11) A, beta = 90.40(1) degrees , and Z = 4. The structure of 1 is composed of one-dimensional (1) infinity{Nd[PS 4] 4} (9-) chains and charge balancing K (+) ions. Within the chains, eight-coordinated Nd (3+) ions, which are mixed with K (+) ions, are connected by [PS 4] (3-) tetrahedra. The crystal structures of 2 and 3 are characterized by anionic chains (1) infinity{Nd[PS 4] 2} (3-) being separated by K (+) or Cs (+) ions. Along each chain the Nd (3+) ions are bridged by [PS 4] (3-) anions. The difference between the structures of 2 and 3 is that in 2 the Nd (3+) ions are coordinated by four edge-sharing [PS 4] (3-) tetrahedra while in 3 each Nd (3+) ion is surrounded by one corner-sharing, one face-sharing, and two edge-sharing [PS 4] (3-) tetrahedra. The structure of 4 is a three-dimensional network with K (+) cations residing in tunnels running along [110] and [110]. The {Nd(1)S 8} polyhedra share common edges with four [PS 4] tetrahedra forming one-dimensional chains (1) infinity{Nd[PS 4] 2} (3-) running along [110] and [110]. The chains are linked by {Nd(2)S 8} polyhedra yielding the final three-dimensional network (3) infinity{Nd[PS 4] 2} (3-). The internal vibrations of both crystallographically independent

  7. STS-84 Mission Specialist Carlos I. Noriega in white room

    Science.gov (United States)

    1997-01-01

    KENNEDY SPACE CENTER, FLA. -- STS-84 Mission Specialist Carlos I. Noriega prepares to enter the Space Shuttle Atlantis at Launch Pad 39A with help from white room closeout crew members. The fourth Shuttle mission of 1997 will be the sixth docking of the Space Shuttle with the Russian Space Station Mir. The commander is Charles J. Precourt. The pilot is Eileen Marie Collins. The five mission specialists are C. Michael Foale, Carlos I. Noriega, Edward Tsang Lu, Jean-Francois Clervoy of the European Space Agency and Elena V. Kondakova of the Russian Space Agency. The planned nine-day mission will include the exchange of Foale for U.S. astronaut and Mir 23 crew member Jerry M. Linenger, who has been on Mir since Jan. 15. Linenger transferred to Mir during the last docking mission, STS-81; he will return to Earth on Atlantis. Foale is slated to remain on Mir for about four months until he is replaced in September by STS-86 Mission Specialist Wendy B. Lawrence. During the five days Atlantis is scheduled to be docked with the Mir, the STS-84 crew and the Mir 23 crew, including two Russian cosmonauts, Commander Vasily Tsibliev and Flight Engineer Alexander Lazutkin, will participate in joint experiments. The STS-84 mission also will involve the transfer of more than 7,300 pounds of water, logistics and science equipment to and from the Mir. Atlantis is carrying a nearly 300-pound oxygen generator to replace one of two Mir units which have experienced malfunctions. The oxygen it generates is used for breathing by the Mir crew.

  8. Regulation by retinoids of luteinizing hormone/chorionic gonadotropin receptor, cholesterol side-chain cleavage cytochrome P-450, 3 beta-hydroxysteroid dehydrogenase/delta (5-4)-isomerase and 17 alpha-hydroxylase/C17-20 lyase cytochrome P-450 messenger ribonucleic acid levels in the K9 mouse Leydig cell line.

    Science.gov (United States)

    Lefèvre, A; Rogier, E; Astraudo, C; Duquenne, C; Finaz, C

    1994-12-01

    Vitamin A is a potent regulator of testicular function. We have reported that retinol (R) and retinoic acid (RA) induced a down regulation of luteinizing hormone/human chorionic gonadotropin (LH/CG) binding sites in K9 Leydig cells. In the present study we evaluated the effect of R and RA on LH/CG receptors, cholesterol side-chain cleavage cytochrome P-450 (P-450 scc), 17 alpha-hydroxylase/C17-20 lyase (P-450 17 alpha) and 3 beta-hydroxysteroid dehydrogenase (3 beta HSD) mRNA levels in K9 mouse Leydig cells. To validate K9 cells as a model for studying Leydig cell steroidogenesis at the molecular level, we first investigated the effect of hCG on mRNA levels of the steroidogenic enzymes. P-450 scc, 3 beta HSD and P-450 17 alpha were expressed constitutively. The addition of 10 ng/ml hCG enhanced mRNA levels for the three genes within 2 h. Maximal accumulation of P-450 scc, P-450 17 alpha and 3 beta HSD mRNA in treated cells represents a 2.5-, 8.5- and 4-fold increase over control values, respectively. P-450 17 alpha expression reached a maximum by 4 h and then declined rapidly to return to control value by 24 h. The pattern of LH/CG receptor mRNAs in K9 cells was very similar to that of MA10 Leydig cells and showed six transcripts of 1.1, 1.6, 1.9, 2.6, 4.2 and 7.0 kb. Treatment of cells with R or RA resulted in a time- and dose-dependent decrease in all six species.(ABSTRACT TRUNCATED AT 250 WORDS)

  9. STS-93 Pilot Ashby signs autographs after mission presentation

    Science.gov (United States)

    1999-01-01

    STS-93 Pilot Jeffrey S. Ashby signs autographs after a mission presentation for KSC employees. The five-day mission primarily released the Chandra X-ray Observatory, allowing scientists from around the world to study some of the most distant, powerful and dynamic objects in the universe. Chandra is expected to provide unique and crucial information on the nature of objects ranging from comets in our solar system to quasars at the edge of the observable universe. Since X-rays are absorbed by the Earth's atmosphere, space-based observatories are necessary to study these phenomena and allow scientists to analyze some of the greatest mysteries of the universe. STS-93 was also the first mission to have a woman, Eileen M. Collins, serving as Shuttle commander.

  10. STS-100 Pilot Ashby suits up for launch

    Science.gov (United States)

    2001-01-01

    KENNEDY SPACE CENTER, Fla. - STS-100 Pilot Jeffrey S. Ashby gives a silent greeting to his wife, Paige, during suitup for launch in the Operations and Checkout Building. The 11-day mission to the International Space Station will deliver and integrate the Spacelab Logistics Pallet/Launch Deployment Assembly, which includes the Space Station Remote Manipulator system and the UHF Antenna. The mission includes two planned spacewalks for installation of the SSRMS, which will be performed by Mission Specialists Scott E. Parazynski and Chris A. Hadfield. The mission is also the inaugural flight of Multi-Purpose Logistics Module Raffaello, carrying resupply stowage racks and resupply/return stowage platforms. Liftoff on mission STS-100 is scheduled at 2:41 p.m. EDT April 19.

  11. STS-84 oxygen generator for Mir on display at SPACEHAB

    Science.gov (United States)

    1997-01-01

    An oxygen generator destined to replace a malfunctioning unit on the Russian Mir Space Station is the object of much curiosity during preflight preparations in the SPACEHAB Payload Processing Facility. A SPACEHAB Double Module on the Space Shuttle Atlantis will carry the oxygen generator to Mir during STS-84, the sixth Shuttle-Mir docking. The nearly 300-pound generator, manufactured by RSC Energia in Russia, will replace one of two Mir units that have been malfunctioning recently. The generator functions by electrolysis, which separates water into its oxygen and hydrogen components. The hydrogen is vented and the oxygen is used for breathing by the Mir crew. The generator is 4.2 feet in length and 1.4 feet in diameter. STS-84, which is planned to include a Mir crew exchange of astronaut C. Michael Foale for Jerry M. Linenger, is targeted for a May 15 liftoff.

  12. STS-95 Pilot Steve Lindsey suits up for launch

    Science.gov (United States)

    1998-01-01

    STS-95 Pilot Steven W. Lindsey tests his flight suit in the Operations and Checkout Building. The final fitting takes place prior to the crew walkout and transport to Launch Pad 39B. Targeted for launch at 2 p.m. EST on Oct. 29, the mission is expected to last 8 days, 21 hours and 49 minutes, and return to KSC at 11:49 a.m. EST on Nov. 7. The STS-95 mission includes research payloads such as the Spartan solar-observing deployable spacecraft, the Hubble Space Telescope Orbital Systems Test Platform, the International Extreme Ultraviolet Hitchhiker, as well as the SPACEHAB single module with experiments on space flight and the aging process.

  13. REPORT FROM THE STS NATIONAL DATABASE WORK FORCE

    Science.gov (United States)

    Overman, David M.; Jacobs, Jeffrey P.; Prager, Richard L.; Wright, Cameron D.; Clarke, David R.; Pasquali, Sara; O’Brien, Sean M.; Dokholyan, Rachel S.; Meehan, Paul; McDonald, Donna E.; Jacobs, Marshall L.; Mavroudis, Constantine; Shahian, David M.

    2013-01-01

    Several distinct definitions of postoperative death have been used in various quality reporting programs. Some have defined a postoperative mortality as a patient who expires while still in the hospital, while others have considered all deaths occurring within a predetermined, standardized time interval after surgery. While if continues to collect mortality data using both these individual definitions, the Society of Thoracic Surgeons (STS) believes that either alone may be inadequate. Accordingly, the STS prefers a more encompassing metric, Operative Mortality, which is defined as (1) all deaths occurring during the hospitalization in which the operation was performed, even if after 30 days; and (2) all deaths occurring after discharge from the hospital, but before the end of the thirtieth postoperative day. This manuscript provides clarification for some uncommon but important scenarios where the correct application of this definition may be problematic. PMID:23799748

  14. STS-47 Endeavour, OV-105, Official crew portrait

    Science.gov (United States)

    1992-01-01

    STS-47 Endeavour, Orbiter Vehicle (OV) 105, Official portrait includes the seven crewmembers wearing launch and entry suits (LESs). These seven crewmembers are currently in training for the STS-47 Spacelab J (SLJ) mission scheduled for later this year. Pictured are (left to right, front) Mission Specialist (MS) Jerome Apt and Pilot Curtis L. Brown, Jr (both holding launch and entry helmets (LEHs)); and (left to right, rear) MS N. Jan Davis, MS and Payload Commander (PLC) Mark C. Lee, Commander Robert L. Gibson, MS Mae C. Jemison, and Japanese Payload Specialist Mamoru Mohri. Mohri is representing the National Space Development Agency of Japan (NASDA). In the background are the flags of the United States (U.S.) and Japan. Portrait was made by NASA JSC contract photographer Robert G. Markowitz.

  15. Post STS-133 Evaluation of Main Flame Deflector Witness Materials

    Science.gov (United States)

    Long, Victoria

    2011-01-01

    NASA and USA Structures engineers submitted main flame deflector witness materials for evaluation after the launch of STS-133. The following items were submitted for analysis: HY-80 steel witness rods, 304 stainless steel caps, tungsten pistons, 17-4 precipitation hardened (PH) stainless steel and A-286 piston sleeves, Medtherm Corporation calorimeters, and Nanmac Corporation thermocouples. All of the items were photographed in order to document their condition after the launch of STS-133, and before they were reinstalled at the launch pad for future launches. The HY -80 witness rods, 304 stainless steel caps, and the piston sleeves were dimensionally measured in order to determine the amount of material lost during launch. Microstructural changes were observed in the HY-80 witness rod and 304 stainless steel cap metallographic samples due to the heat of the launch.

  16. STS-84 M.S. Carlos Noriega suits up

    Science.gov (United States)

    1997-01-01

    STS-84 Mission Specialist Carlos I. Noriega gets assistance from a suit technician as he dons his launch and entry suit during final prelaunch preparations in the Operations and Checkout Building. This will be Noriegas first space flight. Noriega and six other crew members will depart shortly for Launch Pad 39A, where the Space Shuttle Atlantis awaits liftoff on a mission to dock with the Russian Space Station Mir.

  17. STS-97 Crew Interview: Carlos Noriega, MS3

    Science.gov (United States)

    2000-01-01

    The STS-97 Mission Specialist Carlos Noriega is seen being interviewed. He answers questions about his inspiration to become an astronaut, his career path, and his training. He gives details on the mission's goals and significance, its payload, the rendez-vous with the International Space Station (ISS), and what it will be like to work knowing there is already a crew on board the ISS.

  18. STS-74 Mission Cmdr Kenneth D. Cameron suits up

    Science.gov (United States)

    1995-01-01

    STS-74 Commander Kenneth D. Cameron is donning his launch/entry suit in the Operations and Checkout Building as a suit technician lends a helping hand. Cameron and four fellow astronauts are scheduled to depart shortly for Launch Pad 39A, where the Space Shuttle Atlantis awaits a second liftoff attempt during a seven- minute window scheduled to open at approximately 7:30 a.m. EST, Nov. 12.

  19. Three STS 26 astronauts training in the Crew Compartment trainer

    Science.gov (United States)

    1986-01-01

    Three astronauts named in January 1987 as part of a five-member crew for NASA's first flight since the Challenger accident are shown in a photo session of July 1986. Left to right are Astronauts John M. (Mike) Lounge, Richard O. Covey and David C. Hilmers. Lounge and Hilmers will serve as Mission specialists for the STS 26 flight and Covey will serve as pilot. The three are on the middeck of JSC's one-G Crew Compartment Trainer (CCT).

  20. STS-47 crewmembers during KSC terminal countdown demonstration test (TCDT)

    Science.gov (United States)

    1992-01-01

    STS-47 crewmembers participate in terminal countdown demonstration test (TCDT) at Kennedy Space Center (KSC). On KSC Launch Complex (LC) Pad 39B tower, Mission Specialist (MS) Mark C. Lee (wearing sunglasses) points to a distant location as Japanese Payload Specialist Mamoru Mohri (center), and Mission Specialist Mae C. Jemison look on. The crewmembers are standing on the launch tower's slidewire emergency egress system platform.

  1. Unity Module in the Payload Bay for STS-88

    Science.gov (United States)

    1998-01-01

    The Unity module inside the shuttle payload bay of Space Shuttle Endeavour. Endeavour is scheduled to fly on mission STS-88, the first Space Shuttle flight for the assembly of the International Space Station, on December 3, 1998. The primary payload on the mission is the Unity connecting module, which will be mated to the Russian-built Zarya Control Module already in orbit at that time.

  2. Apollo 11 Cmdr Neil Armstrong watches STS-83 launch

    Science.gov (United States)

    1997-01-01

    Apollo 11 Commander Neil A. Armstrong and his wife, Carol, were among the many special NASA STS-83 launch guests who witnessed the liftoff of the Space Shuttle Columbia April 4 at the Banana Creek VIP Viewing Site at KSC. Columbia took off from Launch Pad 39A at 2:20:32 p.m. EST to begin the 16-day Microgravity Science Laboratory-1 (MSL-1) mission.

  3. STS-35 DTO 0634 EDO trash compactor demonstration at JSC

    Science.gov (United States)

    1990-01-01

    STS-35 Development Test Objective (DTO) 0634 Trash Compaction and Retention System Demostration extended duration orbiter (EDO) trash compactor is operated by Project Engineer Fred Abolfathi of Lockheed Engineering and Space Corporation (left) and JSC Man-Systems Division Subsystems Manager J.B. Thomas. The EDO trash compactor will occupy one middeck locker and consists of a geared mechanism that allows manual compaction of wet and dry trash.

  4. STS-103 crew practice emergency egress in the slidewire basket

    Science.gov (United States)

    1999-01-01

    In the slidewire basket on Launch Pad 39B, STS-103 Mission Specialist C. Michael Foale (Ph.D.) gets ready to pull the lever, which will release the basket. With Foale are fellow crew members Mission Specialists Claude Nicollier of Switzerland and John M. Grunsfeld (Ph.D.). The baskets are part of the emergency egress system for persons in the Shuttle vehicle or on the Rotating Service Structure. Seven slidewires extend from the orbiter access arm, with a netted, flatbottom basket suspended from each wire. The STS-103 crew are taking part in Terminal Countdown Demonstration Test (TCDT) activities in preparation for launch. The other crew members taking part are Commander Curtis L. Brown Jr., Pilot Scott J. Kelly, and Mission Specialists Steven L. Smith, and Jean-Frangois Clervoy of France. Clervoy and Nicollier are with the European Space Agency. The TCDT provides the crew with the emergency egress training, opportunities to inspect their mission payloads in the orbiter's payload bay, and simulated countdown exercises. STS-103 is a 'call-up' mission due to the need to replace and repair portions of the Hubble Space Telescope, including the gyroscopes that allow the telescope to point at stars, galaxies and planets. The STS-103 crew will be replacing a Fine Guidance Sensor, an older computer with a new enhanced model, an older data tape recorder with a solid-state digital recorder, a failed spare transmitter with a new one, and degraded insulation on the telescope with new thermal insulation. The crew will also install a Battery Voltage/Temperature Improvement Kit to protect the spacecraft batteries from overcharging and overheating when the telescope goes into a safe mode. Four EVA's are planned to make the necessary repairs and replacements on the telescope. The mission is targeted for launch Dec. 6 at 2:37 a.m. EST.

  5. MS Noriega and Tanner check out STS-97 payload

    Science.gov (United States)

    2000-01-01

    In the Space Station Processing Facility, STS-97 Mission Specialists Carlos Noriega (left) and Joe Tanner check out the mission payload, the P6 integrated truss segment. Mission STS-97 is the sixth construction flight to the International Space Station. The P6 comprises Solar Array Wing-3 and the Integrated Electronic Assembly, to be installed on the International Space Station. The Station's electrical power system will use eight photovoltaic solar arrays, each 112 feet long by 39 feet wide, to convert sunlight to electricity. The solar arrays are mounted on a '''blanket''' that can be folded like an accordion for delivery. Once in orbit, astronauts will deploy the blankets to their full size. Gimbals will be used to rotate the arrays so that they will face the Sun to provide maximum power to the Space Station. The mission includes two spacewalks by Noriega and Tanner to complete the solar array connections. STS-97 is scheduled to launch Nov. 30 at about 10:06 p.m. EST.

  6. STS-93 Pilot Ashby suits up before launch

    Science.gov (United States)

    1999-01-01

    In the Operations and Checkout Building during final launch preparations for the second time, STS-93 Pilot Jeffrey S. Ashby waves after donning his launch and entry suit while a suit tech adjusts his boot. After Space Shuttle Columbia's July 20 launch attempt was scrubbed at the T-7 second mark in the countdown, the launch was rescheduled for Thursday, July 22, at 12:28 a.m. EDT. The target landing date is July 26, 1999, at 11:24 p.m. EDT. STS- 93 is a five-day mission primarily to release the Chandra X-ray Observatory, which will allow scientists from around the world to study some of the most distant, powerful and dynamic objects in the universe. The new telescope is 20 to 50 times more sensitive than any previous X-ray telescope and is expected unlock the secrets of supernovae, quasars and black holes. The STS-93 crew numbers five: Commander Eileen M. Collins, Ashby, and Mission Specialists Stephen A. Hawley (Ph.D.), Catherine G. Coleman (Ph.D.) and Michel Tognini of France, with the Centre National d'Etudes Spatiales (CNES). Collins is the first woman to serve as commander of a shuttle mission.

  7. STS-121: Discovery L-1 Countdown Status Briefing

    Science.gov (United States)

    2006-01-01

    Bruce Buckingham, NASA Public Affairs, introduces Jeff Spaulding, NASA Test Director; Debbie Hahn, STS-121 Payload Manager; and Kathy Winters, Shuttle Weather Officer. Spaulding gives his opening statement on this one day prior to the launching of the Space Shuttle Discovery. He discusses the following topics: 1) Launch of the Space Shuttle Discovery; 2) Weather; 3) Load over of onboard reactants; 4) Hold time for liquid hydrogen; 5) Stowage of Mid-deck completion; 6) Check-out of onboard and ground network systems; 7) Launch windows; 8) Mission duration; 9) Extravehicular (EVA) plans; 10) Space Shuttle landing day; and 11) Scrub turn-around plans. Hahn presents and discusses a short video of the STS-121 payload flow. Kathy Winters gives her weather forecast for launch. She then presents a slide presentation on the following weather conditions for the Space Shuttle Discovery: 1) STS-121 Tanking Forecast; 2) Launch Forecast; 3) SRB Recovery; 4) CONUS Launch; 5) TAL Launch; 6) 24 Hour Delay; 7) CONUS 24 Hour; 8) TAL 24 Hour; 9) 48 Hour Launch; 10) CONUS 48 Hour; and 11) TAL 48 Hour. The briefing ends with a question and answer period from the media.

  8. STS Gene in a Pedigree with X-linked Ichthyosis

    Institute of Scientific and Technical Information of China (English)

    LIU An; XIAO Shengxiang; TAN Shengshun; LEI Xiaobing; ZHANG Jiangan; JIAO Ting; LIU Yan

    2005-01-01

    To investigate the gene mutation in a pedigree with X-linked ichthyosis (XLI) and to explore the relationship between the mutation and its clinical manifestations, genomic DNA of affected members, the normal member of the pedigree and 50 unrelated normal members was extracted with a whole blood genomic DNA extraction kit and the DNA was used as a template for the polymerase chain reaction (PCR)-mediated amplification of exon 1 and exon 10 of the STS gene. hHb6 (human hair basic keratin) gene was used as the internal control. Our results showed that the STS gene was deleted in affected members in the pedigree with X-linked ichthyosis. The normal member of the pedigree and 50 unrelated normal members had no such deletion. The proband and his mother had products in the internal control after PCR amplification. The blank control had no product. It is concluded that deletion of the STS gene existed in this pedigree with X-linked ichthyosis, and it is responsible for the unique skin lesions of X-linked ichthyosis.

  9. STS-103 crew wait inside Discovery for simulated countdown exercise

    Science.gov (United States)

    1999-01-01

    STS-103 Mission Commander Curtis L. Brown Jr. sits inside orbiter Discovery waiting for the start of a simulated countdown exercise. The simulation is part of Terminal Countdown Demonstration Test (TCDT) activities. The TCDT also provides the crew with emergency egress training and opportunities to inspect their mission payload in the orbiter's payload bay. Other crew members taking part in the TCDT are Pilot Scott J. Kelly, and Mission Specialists Steven L. Smith, C. Michael Foale (Ph.D.), John M. Grunsfeld (Ph.D.), Jean-Fran'''ois Clervoy of France, and Claude Nicollier of Switzerland. Clervoy and Nicollier are with the European Space Agency. STS-103 is a 'call-up' mission due to the need to replace and repair portions of the Hubble Space Telescope, including the gyroscopes that allow the telescope to point at stars, galaxies and planets. The STS-103 crew will be replacing a Fine Guidance Sensor, an older computer with a new enhanced model, an older data tape recorder with a solid-state digital recorder, a failed spare transmitter with a new one, and degraded insulation on the telescope with new thermal insulation. The crew will also install a Battery Voltage/Temperature Improvement Kit to protect the spacecraft batteries from overcharging and overheating when the telescope goes into a safe mode. Four EVA's are planned to make the necessary repairs and replacements on the telescope. The mission is targeted for launch Dec. 6 at 2:37 a.m. EST.

  10. STS-103 crew on Pad 39-B during TCDT activities

    Science.gov (United States)

    1999-01-01

    Taking a break during emergency egress training at Launch Pad 39B are (left to right) STS-103 Mission Specialists Jean-Frangois Clervoy of France, Claude Nicollier of Switzerland, Commander Curtis L. Brown Jr., Pilot Scott J. Kelly, and Mission Specialists John M. Grunsfeld (Ph.D.), C. Michael Foale (Ph.D.) and Steven L. Smith. Clervoy and Nicollier are with the European Space Agency. The training is part of Terminal Countdown Demonstration Test (TCDT) activities that also include opportunities to inspect the mission payloads in the orbiter's payload bay and simulated countdown exercises. STS-103 is a 'call-up' mission due to the need to replace and repair portions of the Hubble Space Telescope, including the gyroscopes that allow the telescope to point at stars, galaxies and planets. The STS-103 crew will be replacing a Fine Guidance Sensor, an older computer with a new enhanced model, an older data tape recorder with a solid-state digital recorder, a failed spare transmitter with a new one, and degraded insulation on the telescope with new thermal insulation. The crew will also install a Battery Voltage/Temperature Improvement Kit to protect the spacecraft batteries from overcharging and overheating when the telescope goes into a safe mode. Four EVA's are planned to make the necessary repairs and replacements on the telescope. The mission is targeted for launch Dec. 6 at 2:37 a.m. EST.

  11. STS-103 MS Clervoy tries on oxygen mask

    Science.gov (United States)

    1999-01-01

    In the bunker at Launch Pad 39B, STS-103 Mission Specialist Jean-Frangois Clervoy of France, who is with the European Space Agency (ESA), tries on an oxygen mask during Terminal Countdown Demonstration Test (TCDT) activities. The TCDT provides the crew with emergency egress training, opportunities to inspect their mission payloads in the orbiter's payload bay, and simulated countdown exercises. Other crew members taking part are Commander Curtis L. Brown Jr. and Mission Specialists Steven L. Smith, C. Michael Foale (Ph.D.), John M. Grunsfeld (Ph.D.), plus Claude Nicollier of Switzerland, who is also with ESA. STS-103 is a 'call-up' mission due to the need to replace and repair portions of the Hubble Space Telescope, including the gyroscopes that allow the telescope to point at stars, galaxies and planets. The STS-103 crew will be replacing a Fine Guidance Sensor, an older computer with a new enhanced model, an older data tape recorder with a solid-state digital recorder, a failed spare transmitter with a new one, and degraded insulation on the telescope with new thermal insulation. The crew will also install a Battery Voltage/Temperature Improvement Kit to protect the spacecraft batteries from overcharging and overheating when the telescope goes into a safe mode. Four EVA's are planned to make the necessary repairs and replacements on the telescope. The mission is targeted for launch Dec. 6 at 2:37 a.m. EST.

  12. Longevidade de inflorescências de lírio, de diferentes estádios de colheita, pré-tratadas com sacarose e tiossulfato de prata (STS Longevity of lilly inflorescences harvested at different stages of development and pulsed with sucrose and silver thiosulfate (STS

    Directory of Open Access Journals (Sweden)

    José Geraldo Barbosa

    2006-02-01

    flowers at different ages. They were evaluated at length and opening of the buds, relative water content, content of chlorophyll and longevity. The longevity of lilly flowers variety Ace is influenced by the stage of harvest, with longer longevity for the younger stage, A. Inflorescences harvested at stage A and pulsed with STS had bigger diameter, but the treatment reduced the bud opening and flower quality. The sucrose improved the opening of buds harvested at stage A and increased the flower vase life. Thus the use of sucrose at 5% allowed harvesting the inflorescences at earlier stage, increasing the flower vase life.

  13. Voice identity recognition: functional division of the right STS and its behavioral relevance.

    Science.gov (United States)

    Schall, Sonja; Kiebel, Stefan J; Maess, Burkhard; von Kriegstein, Katharina

    2015-02-01

    The human voice is the primary carrier of speech but also a fingerprint for person identity. Previous neuroimaging studies have revealed that speech and identity recognition is accomplished by partially different neural pathways, despite the perceptual unity of the vocal sound. Importantly, the right STS has been implicated in voice processing, with different contributions of its posterior and anterior parts. However, the time point at which vocal and speech processing diverge is currently unknown. Also, the exact role of the right STS during voice processing is so far unclear because its behavioral relevance has not yet been established. Here, we used the high temporal resolution of magnetoencephalography and a speech task control to pinpoint transient behavioral correlates: we found, at 200 msec after stimulus onset, that activity in right anterior STS predicted behavioral voice recognition performance. At the same time point, the posterior right STS showed increased activity during voice identity recognition in contrast to speech recognition whereas the left mid STS showed the reverse pattern. In contrast to the highly speech-sensitive left STS, the current results highlight the right STS as a key area for voice identity recognition and show that its anatomical-functional division emerges around 200 msec after stimulus onset. We suggest that this time point marks the speech-independent processing of vocal sounds in the posterior STS and their successful mapping to vocal identities in the anterior STS.

  14. An Evaluation of Installation Methods for STS-1 Seismometers

    Science.gov (United States)

    Holcomb, L. Gary; Hutt, Charles R.

    1992-01-01

    INTRODUCTION This report documents the results of a series of experiments conducted by the authors at the Albuquerque Seismological Laboratory (ASl) during the spring and summer of 1991; the object of these experiments was to obtain and document quantitative performance comparisons of three methods of installing STS-1 seismometers. Historically, ASL has installed STS-1 sensors by cementing their thick glass base plates to the concrete floor of the vault (see Peterson and Tilgner, 1985, p 44 and Figure 31, p 51 for the details of this installation technique). This installation technique proved to be fairly satisfactory for the China Digital Seismic Network and for several sets of STS-1 sensors installed in other locations since that time. However, the cementing operation is rather labor intensive and the concrete requires a lengthy (about 1 week) curing time during which the sensor installed on it is noisy. In addition it is difficult to assure that all air bubbles have been removed from the interface between the cement and the glass base plate. If air bubbles are present beneath the plate, horizontal sensors can be unacceptably noisy. Moving a sensor installed in this manner requires the purchase of a new glass base plate because the old plate normally can not be removed without breakage. Therefore, this study was undertaken with the aim of developing an improved method of installing STS-1's. The goals were to develop a method which requires less field site labor during the installation and assures a higher quality installation when finished. In addition, the improved installation technique should promote portability. Two alternate installation techniques were evaluated in this study. One method replaces the cement between the base plate and the vault floor with sand. This method has been used in the French Geoscope program and in several IRIS/IDA installations made by the University of California at San Diego (UCSD) and possibly others. It is easily implemented in

  15. Social contention in Denmark over alternative wind power development paths

    DEFF Research Database (Denmark)

    Nyborg, Sophie; Kirkegaard, Julia Kirch; Clausen, Laura Tolnov

    Through a case-study on the development of a contested wind farm project in the Northern part of Jutland in Denmark, this paper builds on an STS-approach to shed light on the contested acceptability of wind farm development, which has produced controversy and social contention over energy justice....... Wind energy projects on land in Denmark are increasingly subject to social contention. Research and policy are mostly directed towards understanding how 'public acceptance' of current market-driven ways of wind power development can be supported and less on exploring the potentiality of alternative...... paths or understanding processes of coalition formation and reasons for social contention that underlie socio-technical controversies over sustainable transitions. In this paper, we draw on case-study research, inquiring into the contested translation of a Danish wind farm site in the rural area...

  16. PARTICLE SIZE-DEPENDENT PULVERIZATION OF B4C AND GENERATION OF B4C/STS NANOPARTICLES USED FOR NEUTRON ABSORBING COMPOSITES

    Directory of Open Access Journals (Sweden)

    JAEWOO KIM

    2014-10-01

    Full Text Available Pulverization of two different sized micro-B4C particles (∼10 μm and ∼150 μm was investigated using a STS based high energy ball milling system. Shapes, generation of the impurities, and reduction of the particle size dependent on milling time and initial particle size were investigated using various analytic tools including SEM-EDX, XRD, and ICP-MS. Most of impurity was produced during the early stage of milling, and impurity content became independent on the milling time after the saturation. The degree of particle size reduction was also dependent on the initial B4C size. It was found that the STS nanoparticles produced from milling is strongly bounded with the B4C particles forming the B4C/STS composite particles that can be used as a neutron absorbing nanocomposite. Based on the morphological evolution of the milled particles, a schematic pulverization model for the B4C particles was constructed.

  17. STS-103 crew pose in front of Pad 39B

    Science.gov (United States)

    1999-01-01

    During Terminal Countdown Demonstration Test (TDCT) activities at Launch Pad 39B, the STS-103 crew pose in front of the flame trench, which is situated underneath the Mobile Launcher Platform holding Space Shuttle Discovery. Standing left to right are Mission Specialists Claude Nicollier of Switzerland, who is with the European Space Agency (ESA), C. Michael Foale (Ph.D.), John M. Grunsfeld (Ph.D.), Pilot Scott J. Kelly, Commander Curtis L. Brown Jr., and Mission Specialists Jean-Frangois Clervoy of France, also with ESA, and Steven L. Smith. One of the solid rocket boosters and the external tank that are attached to Discovery can be seen in the photo. The flame trench is made of concrete and refractory brick, and contains an orbiter flame deflector on one side and solid rocket booster flame deflector on the other. The deflectors protect the flame trench floor and pad surface from the intense heat of launch. The TCDT provides the crew with emergency egress training, opportunities to inspect their mission payloads in the orbiter's payload bay, and simulated countdown exercises. STS-103 is a 'call-up' mission due to the need to replace and repair portions of the Hubble Space Telescope, including the gyroscopes that allow the telescope to point at stars, galaxies and planets. The STS-103 crew will be replacing a Fine Guidance Sensor, an older computer with a new enhanced model, an older data tape recorder with a solid-state digital recorder, a failed spare transmitter with a new one, and degraded insulation on the telescope with new thermal insulation. The crew will also install a Battery Voltage/Temperature Improvement Kit to protect the spacecraft batteries from overcharging and overheating when the telescope goes into a safe mode. Four EVA's are planned to make the necessary repairs and replacements on the telescope. The mission is targeted for launch Dec. 6 at 2:37 a.m. EST.

  18. STS-103 crew look over payload inside Discovery

    Science.gov (United States)

    1999-01-01

    Members of the STS-103 crew, with representatives from Goddard Space Flight Center, look over the Hubble servicing cargo in the payload bay of Space Shuttle Discovery at Launch Pad 39B. From left are Mission Specialist Steven L. Smith and Claude Nicollier of Switzerland; Steve Pataki and Dave Southwick, with Goddard; and Mission Commander Curtis L. Brown Jr. Inspecting the payload is part of the Terminal Countdown Demonstration Test (TCDT), which also provides the crew with emergency egress training and a simulated countdown exercise. Other crew members taking part in the TCDT are Pilot Scott J. Kelly, and Mission Specialists C. Michael Foale (Ph.D.), John M. Grunsfeld (Ph.D.), and Jean- Fran'''ois Clervoy of France. Clervoy and Nicollier are with the European Space Agency. STS-103 is a 'call-up' mission due to the need to replace and repair portions of the Hubble Space Telescope, including the gyroscopes that allow the telescope to point at stars, galaxies and planets. The STS-103 crew will be replacing a Fine Guidance Sensor, an older computer with a new enhanced model, an older data tape recorder with a solid-state digital recorder, a failed spare transmitter with a new one, and degraded insulation on the telescope with new thermal insulation. The crew will also install a Battery Voltage/Temperature Improvement Kit to protect the spacecraft batteries from overcharging and overheating when the telescope goes into a safe mode. Four EVA's are planned to make the necessary repairs and replacements on the telescope. The mission is targeted for launch Dec. 6 at 2:37 a.m. EST.

  19. Science Education and the Science-Technology-Society (S-T-S) Theme.

    Science.gov (United States)

    Bybee, Rodger W.

    1987-01-01

    Aims to clarify the relationship between science education and the science-technology-society (STS) theme and to develop a justification for the inclusion of the STS theme. Examines the current debate over definitions in science education, and provides an historical perspective of science education's purpose as a social institution. (TW)

  20. On the relationship between wind profiles and the STS ascent structural loads

    Science.gov (United States)

    Smith, Orvel E.; Adelfang, Stanley I.; Whitehead, Douglas S.

    1989-01-01

    The response of STS ascent structural load indicators to the wind profile is analyzed. The load indicator values versus Mach numbers are calculated with algorithms using trajectory information. The ascent load minimum margin concept is used to show that the detailed wind profile structure measured by the Jimsphere wind system is not needed to assess the STS rigid body structural wind loads.

  1. STS-56 Commander Cameron, in LES, during JSC emergency egress training

    Science.gov (United States)

    1992-01-01

    STS-56 Discovery, Orbiter Vehicle (OV) 103, Commander Kenneth Cameron, wearing launch and entry suit (LES), poses at the Crew Compartment Trainer (CCT) side hatch before climbing into the mockup. Cameron, along with the other STS-56 crewmembers, is participating in an emergency egress simulation at JSC's Mockup and Integration Laboratory (MAIL) Bldg 9NE.

  2. STS-44 Atlantis, OV-104, MS Musgrave on FB-SMS middeck during JSC training

    Science.gov (United States)

    1991-01-01

    STS-44 Atlantis, Orbiter Vehicle (OV) 104, Mission Specialist (MS) F. Story Musgrave, wearing lightweight headset (HDST), adjusts controls on communications module mounted on a middeck overhead panel. Musgrave is on the middeck of the Fixed Base (FB) Shuttle Mission Simulator (SMS) located in JSC's Mission Simulation and Training Facility Bldg 5. The STS-44 crewmembers are participating in a flight simulation.

  3. STS-26 MS Hilmers on fixed based (FB) shuttle mission simulator (SMS) middeck

    Science.gov (United States)

    1988-01-01

    STS-26 Discovery, Orbiter Vehicle (OV) 103, Mission Specialist (MS) David C. Hilmers prepares to ascend a ladder representing the interdeck access hatch from the shuttle middeck to the flight deck. The STS-26 crew is training in the fixed base (FB) shuttle mission simulator (SMS) located in JSC Mission Simulation and Training Facility Bldg 5.

  4. STS-26 MS Lounge in fixed based (FB) shuttle mission simulator (SMS)

    Science.gov (United States)

    1988-01-01

    STS-26 Discovery, Orbiter Vehicle (OV) 103, Mission Specialist (MS) John M. Lounge, wearing comunications kit assembly headset and crouched on the aft flight deck, performs checklist inspection during training session. The STS-26 crew is training in the fixed base (FB) shuttle mission simulator (SMS) located in JSC Mission Simulation and Training Facility Bldg 5.

  5. STS-26 crew on fixed based (FB) shuttle mission simulator (SMS) flight deck

    Science.gov (United States)

    1988-01-01

    STS-26 Discovery, Orbiter Vehicle (OV) 103, Commander Frederick H. Hauck (left) and Pilot Richard O. Covey review checklists in their respective stations on the foward flight deck. The STS-26 crew is training in the fixed base (FB) shuttle mission simulator (SMS) located in JSC Mission Simulation and Training Facility Bldg 5.

  6. Official Portrait of STS-52 Canadian Payload Specialist Steve G. MacLean

    Science.gov (United States)

    1992-01-01

    STS-52 Canadian Payload Specialist Steven G. MacLean, wearing a launch and entry suit (LES), poses with a launch and entry helmet (LEH) and Canadian flag for his Official portrait. MacLean representing the Canadian Space Agency (CSA) will fly aboard Columbia, Orbiter Vehicle (OV) 102, for the STS-52 mission.

  7. STS-86 Atlantis rolls out to Pad 39A (full moon on horizon)

    Science.gov (United States)

    1997-01-01

    Shortly before dawn, a red-rimmed moon helps to light the way for the Space Shuttle Atlantis as it rolls out to Launch Pad 39A in preparation for launch of Mission STS-86. STS-86 will be the seventh docking of the Space Shuttle with the Russian Space Station Mir. Liftoff is targeted for no earlier than Sept. 22.

  8. Science-Technology-Society (STS): A New Paradigm in Science Education

    Science.gov (United States)

    Mansour, Nasser

    2009-01-01

    Changes in the past two decades of goals for science education in schools have induced new orientations in science education worldwide. One of the emerging complementary approaches was the science-technology-society (STS) movement. STS has been called the current megatrend in science education. Others have called it a paradigm shift for the field…

  9. STS-54 DSO 802, Educational activities 'Physics of Toys', equipment

    Science.gov (United States)

    1993-01-01

    Toys for STS-54 Detailed Supplementary Objective (DSO) 802, Educational activities 'Physics of Toys', are displayed on a table top. Part of the educational activities onboard Endeavour, Orbiter Vehicle (OV) 105, will include several experiments with these toys. DSO 802 will allow the crewmembers to experiment with the various types of toys in a microgravity environment while talking to pupils who will be able to monitor (via classroom television (TV) sets) the onboard activities at their schools. NOTE: Also labeled the Application Specific Preprogrammed Experiment Culture System Physic of Toys (ASPEC).

  10. STS-112 Commander Ashby in white room before launch

    Science.gov (United States)

    2002-01-01

    KENNEDY SPACE CENTER, FLA. - -- In the White Room at Launch Pad 39B, STS-112 Commander Jeffrey Ashby receives assistance with his spacesuit before boarding Space Shuttle Atlantis. Liftoff is schedued for 3:46 p.m. EDT. Along with a crew of six, Atlantis will carry the S1 Integrated Truss Structure and the Crew and Equipment Translation Aid (CETA) Cart A to the International Space Station (ISS). The CETA is the first of two human-powered carts that will ride along the ISS railway, providing mobile work platforms for future spacewalking astronauts. On the 11-day mission, three spacewalks are planned to attach the S1 truss.

  11. STS-74 Cmdr Kenneth D. Cameron in white room

    Science.gov (United States)

    1995-01-01

    At Launch Pad 39A, STS-74 Commander Kenneth D. Cameron finishes preparing his launch/entry suit for flight as a team of white room closeout crew personnel assist him. From left are KSC Lockheed closeout crew lead Mike Mangione; KSC NASA quality assurance technician Eartha Shoemaker; Johnson Space Center Lockheed suit technician Ray Villalobos; JSC NASA suit tech Jean Alexander (behind Cameron); and astronaut Steve Smith.The closeout crew will help Cameron into the cockpit of the orbiter Atlantis, scheduled for liftoff at about 7:30 a.m. EST, Nov. 12.

  12. STS-54 DSO 802, Educational activities 'Physics of Toys', equipment

    Science.gov (United States)

    1993-01-01

    Toys for STS-54 Detailed Supplementary Objective (DSO) 802, Educational activities 'Physics of Toys', are displayed on a table top. Part of the educational activities onboard Endeavour, Orbiter Vehicle (OV) 105, will include several experiments with these toys. DSO 802 will allow the crewmembers to experiment with the various types of toys in a microgravity environment while talking to pupils who will be able to monitor (via classroom television (TV) sets) the onboard activities at their schools. Among the toys seen here are a friction car and loop track, paper eagle, and a balloon helicopter. NOTE: also labeled the Application Specific Preprogrammed Experiment Culture System Physics of Toys (ASPEC).

  13. STS-47 crew extinquishes fire during JSC fire fighting exercises

    Science.gov (United States)

    1992-01-01

    STS-47 Endeavour, Orbiter Vehicle (OV) 105, crewmembers lined up along water hoses direct spray at fire blazing in JSC's Fire Training Pit. At the left are backup Payload Specialist Stan Koszelak, holding the hose nozzle, and Mission Specialist (MS) N. Jan Davis. Manning the hose on the right are backup Payload Specialist Takao Doi, holding the hose nozzle, followed by Commander Robert L. Gibson, Payload Specialist Mamoru Mohri, and MS Jerome Apt. Guiding the teams are MS Mae C. Jemison (front) and a veteran fire fighter and instructor (center). Doi and Mohri represent Japan's National Space Development Agency (NASDA). The Fire Training Pit is located across from the Gilruth Center Bldg 207.

  14. STS-110 Commander Bloomfield during TCDT in Atlantis

    Science.gov (United States)

    2002-01-01

    KENNEDY SPACE CENTER, FLA. -- STS-110 Commander Michael Bloomfield settles into his seat in Space Shuttle Atlantis as he prepares for a simulated launch countdown. The simulation is part of Terminal Countdown Demonstration Test activities. TCDT also includes emergency egress training and is held at KSC prior to each Space Shuttle flight. Scheduled for launch April 4, the 11-day mission will feature Shuttle Atlantis docking with the International Space Station (ISS) and delivering the S0 truss, the centerpiece-segment of the primary truss structure that will eventually extend over 300 feet.

  15. STS-110 Pilot Frick and Commander Bloomfield in slidewire basket

    Science.gov (United States)

    2002-01-01

    KENNEDY SPACE CENTER, FLA. -- STS-110 Pilot Stephen N. Frick and Commander Michael J. Bloomfield climb into the slidewire basket, part of emergency egress equipment on the launch pad.. The crew is taking part in Terminal Countdown Demonstration Test activities, which also include a simulated launch countdown, held at KSC prior to each Space Shuttle flight. Scheduled for launch April 4, the 11-day mission will feature Shuttle Atlantis docking with the International Space Station (ISS) and delivering the S0 truss, the centerpiece-segment of the primary truss structure that will eventually extend over 300 feet.

  16. STS-110 Pilot Frick and Commander Bloomfield during TCDT

    Science.gov (United States)

    2002-01-01

    KENNEDY SPACE CENTER, FLA. -- As part of emergency egress training, STS-110 Pilot Stephen N. Frick and Commander Michael J. Bloomfield head for the slidewire basket on the Fixed Service Structure. The crew is taking part in Terminal Countdown Demonstration Test activities, which also include a simulated launch countdown, held at KSC prior to each Space Shuttle flight. Scheduled for launch April 4, the 11-day mission will feature Shuttle Atlantis docking with the International Space Station (ISS) and delivering the S0 truss, the centerpiece-segment of the primary truss structure that will eventually extend over 300 feet.

  17. Impact of STS Issue Oriented Instruction on Pre-Service Elementary Teachers' Views and Perceptions of Science, Technology, and Society

    Science.gov (United States)

    Amirshokoohi, Aidin

    2016-01-01

    The purpose of the study was to investigate the impact of Science, Technology, Society (STS) issue oriented science methods course on pre-service teachers' views and perceptions toward STS issues and instruction as well as their levels of environmental literacy. The STS issue oriented curriculum was designed to help pre-service teachers improve…

  18. 77 FR 75628 - STS Hydropower, Ltd., Dan River, Inc., and City of Danville, VA; Notice of Application for...

    Science.gov (United States)

    2012-12-21

    ... Energy Regulatory Commission STS Hydropower, Ltd., Dan River, Inc., and City of Danville, VA; Notice of..., 2012, Jeoffrey L. Burtch, as Chapter 7 Bankruptcy Trustee for Dan River, Inc. and STS Hydropower, Ltd... the license for the Schoolfield Hydroelectric Project from Dan River, Inc. and STS Hydropower, Ltd. to...

  19. STS-31 Mission Onboard Photograph-Hubble Space Telescope

    Science.gov (United States)

    1990-01-01

    In this photograph, the Hubble Space Telescope (HST) was being deployed on April 25, 1990. The photograph was taken by the IMAX Cargo Bay Camera (ICBC) mounted in a container on the port side of the Space Shuttle orbiter Discovery (STS-31 mission). The purpose of the HST, the most complex and sensitive optical telescope ever made, is to study the cosmos from a low-Earth orbit for 15 years or more. The HST provides fine detail imaging, produces ultraviolet images and spectra, and detects very faint objects. Two months after its deployment in space, scientists detected a 2-micron spherical aberration in the primary mirror of the HST that affected the telescope's ability to focus faint light sources into a precise point. This imperfection was very slight, one-fiftieth of the width of a human hair. A scheduled Space Service servicing mission (STS-61) in 1993 permitted scientists to correct the problem. During four spacewalks, new instruments were installed into the HST that had optical corrections. The Marshall Space Flight Center had responsibility for design, development, and construction of the HST. The Perkin-Elmer Corporation, in Danbury, Cornecticut, developed the optical system and guidance sensors. Photo Credit: NASA/Smithsonian Institution/Lockheed Corporation.

  20. STS-97 MS Carlos Noriega suits up for launch

    Science.gov (United States)

    2000-01-01

    STS-97 Mission Specialist Carlos Noriega appears relaxed as he dons his launch and entry suit. This is his second Shuttle flight. Mission STS-97 is the sixth construction flight to the International Space Station. It is transporting the P6 Integrated Truss Structure that comprises Solar Array Wing-3 and the Integrated Electronic Assembly, to be installed on the Space Station. The solar arrays are mounted on a '''blanket''' that can be folded like an accordion for delivery. Once in orbit, astronauts will deploy the blankets to their full size. The 11-day mission includes two spacewalks to complete the solar array connections. The Station'''s electrical power system will use eight photovoltaic solar arrays, each 112 feet long by 39 feet wide, to convert sunlight to electricity. Gimbals will be used to rotate the arrays so that they will face the Sun to provide maximum power to the Space Station. Launch is scheduled for Nov. 30 at 10:06 p.m. EST.

  1. STS-103 Discovery crawls to Launch Pad 39B

    Science.gov (United States)

    1999-01-01

    With the American flag flapping in the morning breeze, Space Shuttle Discovery across the turn basin makes its 4.2-mile (6.8 kilometer) crawl to Launch Pad 39B (background, left) atop the mobile launcher platform and crawler transporter. Once at the pad, the orbiter, external tank and solid rocket boosters will undergo final preparations for the STS-103 launch targeted for Dec. 6, 1999, at 2:37 a.m. EST. The mission is a 'call-up' due to the need to replace and repair portions of the Hubble Space Telescope. Although Hubble is operating normally and conducting its scientific observations, only three of its six gyroscopes are working properly. Four EVA's are planned to make the necessary repairs and replacements on the telescope. The STS-103 crew members are Commander Curtis L. Brown Jr., Pilot Scott J. Kelly, Steven L. Smith, C. Michael Foale (Ph.D.), John M. Grunsfeld (Ph.D.), and Claude Nicollier of Switzerland and Jean-Frangois Clervoy of France, both with the European Space Agency.

  2. STS-100 Pilot Ashby arrives at KSC for TCDT activities

    Science.gov (United States)

    2001-01-01

    KENNEDY SPACE CENTER, Fla. - STS-100 Pilot Jeffrey S. Ashby arrives at KSC for Terminal Countdown Demonstration Test activities. He and the rest of the crew Commander Kent V. Rominger and Mission Specialists Chris Hadfield, Scott E. Parazynski, John L. Phillips, Umberto Guidoni and Yuri Lonchakov -- are going to be taking part in emergency escape training at the pad, equipment familiarization and a simulated launch countdown. An international crew, Hadfield is with the Canadian Space Agency, Guidoni the European Space Agency and Lonchakov the Russian Aviation and Space Agency. The mission is carrying the Multi-Purpose Logistics Module Raffaello and the Canadian robotic arm, SSRMS, to the International Space Station. Raffaello carries six system racks and two storage racks for the U.S. Lab. The SSRMS is crucial to the continued assembly of the orbiting complex and has a unique ability to switch ends as it works, '''inchworming''' along the Station'''s exterior. Launch of mission STS-100 is scheduled for April 19 at 2:41 p.m. EDT from Launch Pad 39A.

  3. ISS and STS Commercial Off-the-Shelf Router Testing

    Science.gov (United States)

    Ivancie, William D.; Bell, Terry L.; Shell, Dan

    2002-01-01

    This report documents the results of testing performed with commercial off-the-shelf (COTS) routers and Internet Protocols (IPs) to determine if COTS equipment and IP could be utilized to upgrade NASA's current Space Transportation System (STS), the Shuttle, and the International Space Station communication infrastructure. Testing was performed by NASA Glenn Research Center (GRC) personnel within the Electronic Systems Test Laboratory (ESTE) with cooperation from the Mission Operations Directorate (MOD) Qualification and Utilization of Electronic System Technology (QUEST) personnel. The ESTE testing occurred between November 1 and 9, 2000. Additional testing was performed at NASA Glenn Research Center in a laboratory environment with equipment configured to emulate the STS. This report documents those tests and includes detailed test procedures, equipment interface requirements, test configurations and test results. The tests showed that a COTS router and standard Transmission Control Protocols and Internet Protocols (TCP/IP) could be used for both the Shuttle and the Space Station if near-error-free radio links are provided.

  4. Subsidy of a transforming pedagogical practice: contribution of STS view

    Directory of Open Access Journals (Sweden)

    Marcia Regina Carletto

    2011-04-01

    Full Text Available This study aims to enrich the reflection concerning the insertion of the STS (Society, Technology and Science approach and its implications in Science teaching. The methodology used was qualitative research of interpretative nature and occurred during the development of the subject technological principles in cooperation with Science, Math, Portuguese, History and Sociology. The research had as target students from five groups of High School from a public teaching Institution in Ponta Grossa - PR. The problem question that guided the research and served as background for the presentation of this text, concerns the possibilities of using the STS focus for the implementation of a differentiated teaching. The methodological options were defined based on participative planning, a theoretical reflection and limitations which had been found. The results relate to qualitative contributions to the teaching practice and to the teaching-learning process on Science teaching, such as: curricular integration, high level of motivation and students participation, improvement of analysis capability, argumentation and intervention, deeper understanding of the Science nature, the technological scientific process and its social and environmental repercussion.

  5. STS-97 Pilot Mike Bloomfield suits up for launch

    Science.gov (United States)

    2000-01-01

    STS-97 Pilot Michael Bloomfield signals thumbs up for launch after donning his launch and entry suit. This is his second Shuttle flight. Mission STS-97 is the sixth construction flight to the International Space Station. It is transporting the P6 Integrated Truss Structure that comprises Solar Array Wing-3 and the Integrated Electronic Assembly, to be installed on the Space Station. The solar arrays are mounted on a '''blanket''' that can be folded like an accordion for delivery. Once in orbit, astronauts will deploy the blankets to their full size. The 11-day mission includes two spacewalks to complete the solar array connections. The Station'''s electrical power system will use eight photovoltaic solar arrays, each 112 feet long by 39 feet wide, to convert sunlight to electricity. Gimbals will be used to rotate the arrays so that they will face the Sun to provide maximum power to the Space Station. Launch is scheduled for Nov. 30 at 10:06 p.m. EST.

  6. STS-114 Crew Interviews: 1. Eileen Collins 2. Wendy Lawrence

    Science.gov (United States)

    2005-01-01

    1) STS-114 Commander Eileen Collins emphasized her love for teaching, respect for teachers, and her plan to go back to teaching again someday. Her solid background in Math and Science, focus on her interests, with great support from her family, and great training and support during her career with the Air Force gave her confidence in pursuing her dream to become an astronaut. Commander Collins shares her thoughts on the Columbia, details the various flight operations and crew tasks that will take place during the mission and the importance of Shuttle missions to the International Space Station and space exploration. 2) STS-114 Mission Specialist Wendy Lawrence first dreamed of becoming an astronaut when she watched Neil Armstrong walk on the moon from their black and white TV set. She majored in Engineering and became a Navy pilot. She shares her thoughts on the Columbia, details her major role as the crew in charge of all the transfer operations; getting the MPLM unpacked and repacked; and the importance of Shuttle missions to the International Space Station and space exploration.

  7. Digital Content Strategies

    OpenAIRE

    Daniel Halbheer; Florian Stahl; Oded Koenigsberg; Lehmann, Donald R

    2013-01-01

    This paper studies content strategies for online publishers of digital information goods. It examines sampling strategies and compares their performance to paid content and free content strategies. A sampling strategy, where some of the content is offered for free and consumers are charged for access to the rest, is known as a "metered model" in the newspaper industry. We analyze optimal decisions concerning the size of the sample and the price of the paid content when sampling serves the dua...

  8. K9 water searches: scent and scent transport considerations.

    Science.gov (United States)

    Osterkamp, Tom

    2011-07-01

    Increased use of water search dogs for detecting submerged bodies has created the need for a better understanding of scent emanating from the bodies and how it transits the water to the dog's nose. A review of recent literature identifies likely scent sources, potential scent transport processes, and research needs. Scent sources include gases in bubbles or dissolved in the water, liquids as buoyant plumes and droplets or dissolved in the water, and solids consisting of buoyant particulates with secretions, bacteria, and body fluids. Potential transport processes through the water include buoyancy, entrainment, and turbulence. Transport processes from the water surface into the air include volatilization and evaporation enhanced by bubble bursting, breaking waves, splashing, and wind spray. Implications for the use of water search dogs are examined. Observations of submerged, decomposing bodies are needed to quantify the physical and chemical characteristics of the scent and scent transport processes.

  9. Crystallization of proteinase K complexed with substrate analogue peptides on US space missions STS-91 and STS-95

    Science.gov (United States)

    Eschenburg, Susanne; Degenhardt, Michael; Moore, Karen; DeLucas, Lawrence J.; Peters, Klaus; Fittkau, Siegfried; Weber, Wolfgang; Betzel, Christian

    2000-01-01

    Crystals of proteinase K in complex with synthetic substrate analogues have been grown under microgravity on the US space shuttle missions STS-91 and STS-95 using the vapor diffusion apparatus (c-VDA) supplied by the Center for Macromolecular Crystallography at the University of Alabama at Birmingham. The crystals obtained under microgravity are compared with those grown simultanously on ground in identical c-VDA reactors and in conventional hanging-drop set-ups. The diffraction quality of space- and ground-grown crystals has been assessed by collecting complete data sets with a conventional X-ray source and with synchrotron radiation. Crystals grown in microgravity are clearly superior to those grown in the identical hardware on earth in terms of crystal habit and diffraction power. In comparison to best terrestrial crystals obtained in conventional hanging-drop set-ups the differences in crystal size and diffraction quality are less, but still confirm the benefit of microgravity for the crystallization of proteinase K-substrate analogue complexes.

  10. How Science/Technology/Society relations are approached in the contents of organic functions in high school chemistry

    Directory of Open Access Journals (Sweden)

    Carmem Lúcia Costa Amaral

    2009-03-01

    Full Text Available This paper aimed to verify how the relation between Science/Technology/Society (STS is present in Chemistry textbooks recommended by the Brazilian Ministry of Education. The interest in textbooks is due to the fact that they constitute important resources used by teachers to prepare their classes. Thus, researches in this area are necessary to indicate how the authors could improve the quality of their books. We believe that one way to do this is the introduction of the STS relation, which took place because of the necessity in establishing new ways of teaching, specially the teaching of science. One of the goals of STS education is to create conditions to develop abilities and competences that qualify the students for discussions concerning scientific and technologic questions of everyday life. The analysis of testbooks used the descriptors and indicators developed by Fracalanza and Megid-Neto (2006 as reference. In order to carry out the study, we chose the area of Organic Chemistry, more specifically contents referring to organic functions, with great use in society. In general, the results showed that STS relations are not totally observed in the investigated contents, contributing to the development of Chemistry classes through activities apart from the students social context.

  11. STS-97 Mission Specialist Noriega during pre-pack and fit check

    Science.gov (United States)

    2000-01-01

    STS-97 Mission Specialist Carlos Noriega gets help with his boots from suit technician Shelly Grick-Agrella during pre-pack and fit check. Mission STS-97 is the sixth construction flight to the International Space Station. Its payload includes the P6 Integrated Truss Structure and a photovoltaic (PV) module, with giant solar arrays that will provide power to the Station. The mission includes two spacewalks to complete the solar array connections. STS-97 is scheduled to launch Nov. 30 at about 10:06 p.m. EST.

  12. STS-97 Pilot Bloomfield talks to media after arrival for launch

    Science.gov (United States)

    2000-01-01

    After their arrival at the Shuttle Landing Facility, the STS-97 crew gather to address the media. At the microphone is Pilot Michael Bloomfield. Behind him stand Commander Brent Jett and Mission Specialists Joseph Tanner, Carolos Noriega and Marc Garneau, who is with the Canadian Space Agency. Mission STS-97is the sixth construction flight to the International Space Station. Its payload includes the P6 Integrated Truss Structure and a photovoltaic (PV) module, with giant solar arrays that will provide power to the Station. The mission includes two spacewalks to complete the solar array connections. STS-97 is scheduled to launch Nov. 30 at about 10:06 p.m. EST.

  13. STS-97 Pilot Bloomfield during pre-pack and fit check

    Science.gov (United States)

    2000-01-01

    STS-97 Pilot Michael Bloomfield gets help with his boots from suit technician Steve Clendenin during pre-pack and fit check. Mission STS-97 is the sixth construction flight to the International Space Station. Its payload includes the P6 Integrated Truss Structure and a photovoltaic (PV) module, with giant solar arrays that will provide power to the Station. The mission includes two spacewalks to complete the solar array connections. STS-97 is scheduled to launch Nov. 30 at about 10:06 p.m. EST.

  14. Free zone electrophoresis simulation of static column electrophoresis in microgravity on shuttle flight STS-3

    Science.gov (United States)

    Todd, P. W.; Hjerten, S.

    1985-01-01

    Experiments were designed to replicate, as closely as possible in 1-G, the conditions of the STS-3 red blood cell (RBC) experiments. Free zone electrophoresis was the method of choice, since it minimizes the role of gravity in cell migration. The physical conditions of the STS-3 experiments were used, and human and rabbit RBC's fixed by the same method were the test particles. The effects of cell concentration, electroosmotic mobility, and sample composition were tested in order to seek explanations for the STS-3 results and to provide data on cell concentration effects for future zero-G separation on the continuous-flow zero-G electrophoretics separator.

  15. Endeavour lands at Edwards AFB, ending mission STS-100

    Science.gov (United States)

    2001-01-01

    NASA/Edwards AFB, Calif. -- After landing at Edwards Air Force Base, Calif., the STS-100 crew poses for a photograph in front of orbiter Endeavour, which successfully launched them to the International Space Station and returned them to Earth. They are (left to right) Mission Specialists John Phillips, Umberto Guidoni and Chris Hadfield; Pilot Jeffrey Ashby; Commander Kent Rominger; and Mission Specialists Yuri Lonchakov and Scott Parazynski. Guidoni is with the European Space Agency, Hadfield with the Canadian Space Agency and Lonchakov with the Russian Aviation and Space Agency. The orbiter and crew logged about 4.9 million statute miles in 186 orbits. Due to unfavorable weather conditions, landing at KSC was waved off. The landing marked the third consecutive landing at EAFB.

  16. Development of baseline random vibration criteria for STS pallet payloads

    Science.gov (United States)

    On, F. J.

    1983-01-01

    This paper presents a statistical evaluation of measured random vibration response data obtained from the Office of Space Science-1 (OSS-1) pallet payload. The data were measured during the acoustic test simulation (September 1980) and the ascent phase of the flight of STS-3, Orbiter 102 (launched from the Kennedy Space Center on March 22, 1982). Acoustic test efficiency factors are evaluated based on the Dynamic, Acoustic and Thermal Environments (DATE) instrumentation as the source of the measured vibration data. Test efficiency correction to test data is applied in the extrapolation of non-DATE acoustic test data to increase sample population size for improved statistical evaluation. For baseline criteria evaluation and development, data are grouped in accordance with the payload 'zone' in which the component is mounted.

  17. STS-26 crew in JSC Shuttle Mockup and Integration Laboratory

    Science.gov (United States)

    1988-01-01

    STS-26 Discovery, Orbiter Vehicle (OV) 103, crewmembers have donned their new (navy blue) partial pressure suits (launch and entry suits (LESs)) for a training exercise in JSC's Shuttle Mockup and Integration Laboratory Bldg 9A. Commander Frederick H. Hauck is in the center foreground. Hauck is flanked by fellow crewmembers (left to right) Mission Specialist (MS) John M. Lounge, MS George D. Nelson, Pilot Richard O. Covey, and MS David C. Hilmers. Astronaut Steven R. Nagel, not assigned as crewmember but assisting in training, is at far right. During Crew Station Review (CSR) #3, the crew is scheduled to check out the new partial pressure suits and crew escape system (CES) configurations to evaluate crew equipment and procedures related to emergency egress methods and proposed crew escape options.

  18. STS-34 MS Chang-Diaz records onboard activity with 16mm camera

    Science.gov (United States)

    1989-01-01

    STS-34 Mission Specialist (MS) Franklin R. Chang-Diaz records forward flight deck activity with ARRIFLEX 16mm camera onboard Atlantis, Orbiter Vehicle (OV) 104. In the background, MS Shannon W. Lucid works at aft flight deck payload station.

  19. STS-75 Chang-Diaz and MS Jeff Hoffman in White Room

    Science.gov (United States)

    1996-01-01

    STS-75 Payload Commander Franklin R. Chang-Diaz (center) and Mission Specialist Jeffrey A. Hoffman (right) prepare to enter the Space Shuttle Columbia at Launch Pad 39B with assistance from the white room closeout crew.

  20. STS-35 Pilot Gardner in sleep station compartment on OV-102's middeck

    Science.gov (United States)

    1990-01-01

    STS-35 Pilot Guy S. Gardner demonstrates the bunk-style sleep compartments onboard Columbia's, Orbiter Vehicle (OV) 102's, middeck. Gardner uses bunk #2 of the sleep station located against the middeck starboard wall.

  1. Improving Indicators in a Brazilian Hospital Through Quality-Improvement Programs Based on STS Database Reports

    Directory of Open Access Journals (Sweden)

    Pedro Gabriel Melo de Barros e Silva

    2015-12-01

    Full Text Available ABSTRACT OBJECTIVE: To report the initial changes after quality-improvement programs based on STS-database in a Brazilian hospital. METHODS: Since 2011 a Brazilian hospital has joined STS-Database and in 2012 multifaceted actions based on STS reports were implemented aiming reductions in the time of mechanical ventilation and in the intensive care stay and also improvements in evidence-based perioperative therapies among patients who underwent coronary artery bypass graft surgeries. RESULTS: All the 947 patients submitted to coronary artery bypass graft surgeries from July 2011 to June 2014 were analyzed and there was an improvement in all the three target endpoints after the implementation of the quality-improvement program but the reduction in time on mechanical ventilation was not statistically significant after adjusting for prognostic characteristics. CONCLUSION: The initial experience with STS registry in a Brazilian hospital was associated with improvement in most of targeted quality-indicators.

  2. Improving epistemological beliefs and moral judgment through an STS-based science ethics education program.

    Science.gov (United States)

    Han, Hyemin; Jeong, Changwoo

    2014-03-01

    This study develops a Science-Technology-Society (STS)-based science ethics education program for high school students majoring in or planning to major in science and engineering. Our education program includes the fields of philosophy, history, sociology and ethics of science and technology, and other STS-related theories. We expected our STS-based science ethics education program to promote students' epistemological beliefs and moral judgment development. These psychological constructs are needed to properly solve complicated moral and social dilemmas in the fields of science and engineering. We applied this program to a group of Korean high school science students gifted in science and engineering. To measure the effects of this program, we used an essay-based qualitative measurement. The results indicate that there was significant development in both epistemological beliefs and moral judgment. In closing, we briefly discuss the need to develop epistemological beliefs and moral judgment using an STS-based science ethics education program.

  3. X-linked ichthyosis without STS deficiency: Clinical, genetical, and molecular studies

    Energy Technology Data Exchange (ETDEWEB)

    Robledo, R.; Melis, P.; Schillinger, E.; Siniscalco, M. [Istituto di Genetica Molecolare del, Trieste (Italy)] [and others

    1995-11-06

    We report on a Sardinian pedigree with congenital ichthyosis associated with normal levels of steroid sulfatase and a normal molecular pattern, as detectable with a cDNA probe for the steroid sulfatase (STS) gene. Though the pattern of transmission of the disease is consistent with X-linked recessive inheritance, this form of ichthyosis was found to segregate independently of genetic polymorphisms detected by probes of the region Xp22.3, where the STS locus has been mapped. The search for close genetic linkages with other polymorphic markers scattered along the entire X chromosome has so far been fruitless. For the time being, the main conclusion derived from these data is that STS deficiency is not a sine qua non for X-linked ichthyosis which may also result from a mutational event at an X-chromosomal site genetically unlinked to the STS locus. 16 refs., 4 figs.

  4. STS 41-D mission specialist Judith Resnik trains on the RMS

    Science.gov (United States)

    1983-01-01

    STS 41-D mission specialist Judith Resnik prepares for training on the remote manipulator system (RSM) on board the shuttle mission simulator (SMS). She is on the SMS aft deck facing the RMS translation hand control and overhead starboard window.

  5. STS-75 Chang-Diaz and MS Jeff Hoffman in White Room

    Science.gov (United States)

    1996-01-01

    STS-75 Payload Commander Franklin R. Chang-Diaz (center) and Mission Specialist Jeffrey A. Hoffman (right) prepare to enter the Space Shuttle Columbia at Launch Pad 39B with assistance from the white room closeout crew.

  6. STS-56 Commander Cameron, in LES, with sky genie during JSC egress training

    Science.gov (United States)

    1992-01-01

    STS-56 Discovery, Orbiter Vehicle (OV) 103, Commander Kenneth Cameron, wearing launch and entry suit (LES), gives the sky-genie escape device a tug as training instructor Kenneth D. Trujillo holds it in position and explains its operation. Cameron, along with the other STS-56 crewmembers, is briefed on emergency egress procedures at JSC's Mockup and Integration Laboratory (MAIL) Bldg 9NE prior to a training simulation. The sky-genie is carried on all Space Shuttle flights for emergency egress purposes.

  7. STS-26 Commander Hauck in fixed based (FB) shuttle mission simulator (SMS)

    Science.gov (United States)

    1988-01-01

    STS-26 Discovery, Orbiter Vehicle (OV) 103, Commander Frederick H. Hauck, wearing comunications kit assembly headset and seated in the commanders seat on forward flight deck, looks over his shoulder toward the aft flight deck. A flight data file (FDF) notebook rests on his lap. The STS-26 crew is training in the fixed base (FB) shuttle mission simulator (SMS) located in JSC Mission Simulation and Training Facility Bldg 5.

  8. STS-38 Mission Specialist (MS) Robert C. Springer dons EMU in JSC's WETF

    Science.gov (United States)

    1990-01-01

    STS-38 Mission Specialist (MS) Robert C. Springer, wearing extravehicular mobility unit (EMU), fastens the strap on his communications carrier assembly (CCA) cap during suit donning in JSC's Weightless Environment Training Facility (WETF) Bldg 29. Positioned on the WETF platform at pool side, Springer is preparing for an underwater extravehicular activity (EVA) simulation. During the training exercise, Springer will rehearse contingency EVA procedures for the STS-38 mission aboard Atlantis, Orbiter Vehicle (OV) 104.

  9. STS-38 Mission Specialist (MS) Robert C. Springer dons EMU in JSC's WETF

    Science.gov (United States)

    1990-01-01

    STS-38 Mission Specialist (MS) Robert C. Springer dons extravehicular mobility unit (EMU) upper torso with technicians' assistance in JSC's Weightless Environment Training Facility (WETF) Bldg 29. Positioned on the WETF platform at pool side, Springer is preparing for an underwater extravehicular activity (EVA) simulation. During the training session, Springer will rehearse contingency EVA procedures for the STS-38 mission aboard Atlantis, Orbiter Vehicle (OV) 104.

  10. STS-86 Pilot Bloomfield feels heat from a tire after landing

    Science.gov (United States)

    1997-01-01

    STS-86 Pilot Michael J. Bloomfield, the only space rookie on the crew, feels the heat from a tire on the orbiter Atlantis more than an hour after the landing on KSCs Runway 15. The nearly 11- day mission ended with main gear touchdown at 5:55:09 p.m. EDT, Oct. 6, 1997. STS-86 was the seventh docking of the Space Shuttle with the Russian Space Station Mir.

  11. PUMA promotes Bax translocation in FOXO3a-dependent pathway during STS-induced apoptosis

    Science.gov (United States)

    Zhang, Yingjie; Chen, Qun

    2009-08-01

    PUMA (p53 up-regulated modulator of apoptosis, also called Bbc3) was first identified as a BH3-only Bcl-2 family protein that is transcriptionally up-regulated by p53 and activated upon p53-dependent apoptotic stimuli, such as treatment with DNA-damaging drugs or UV irradiation. Recently studies have been shown that Puma is also up-regulated in response to certain p53-independent apoptotic stimuli, such as growth factor deprivation or treatment with glucocorticoids or STS (staurosporine). However, the molecular mechanisms of PUMA up-regulation and how PUMA functions in response to p53-independent apoptotic stimuli remain poorly understood. In this study, based on real-time single cell analysis, flow cytometry and western blotting technique, we investigated the function of PUMA in living human lung adenocarcinoma cells (ASTC-a-1) after STS treatment. Our results show that FOXO3a was activated by STS stimulation and then translocated from cytosol to nucleus. The expression of PUMA was up-regulated via a FOXO3a-dependent manner after STS treatment, while p53 had little function in this process. Moreover, cell apoptosis and Bax translocation induced by STS were not blocked by Pifithrin-α (p53 inhibitor), which suggested that p53 was not involved in this signaling pathway. Taken together, these results indicate that PUMA promoted Bax translocation in a FOXO3a-dependment pathway during STS-induced apoptosis, while p53 was dispensable in this process.

  12. Virtual reconstruction of the Australopithecus africanus pelvis Sts 65 with implications for obstetrics and locomotion.

    Science.gov (United States)

    Claxton, Alexander G; Hammond, Ashley S; Romano, Julia; Oleinik, Ekaterina; DeSilva, Jeremy M

    2016-10-01

    Characterizing australopith pelvic morphology has been difficult in part because of limited fossilized pelvic material. Here, we reassess the morphology of an under-studied adult right ilium and pubis (Sts 65) from Member 4 of Sterkfontein, South Africa, and provide a hypothetical digital reconstruction of its overall pelvic morphology. The small size of the pelvis, presence of a preauricular sulcus, and shape of the sciatic notch allow us to agree with past interpretations that Sts 65 likely belonged to a female. The morphology of the iliac pillar, while not as substantial as in Homo, is more robust than in A.L. 288-1 and Sts 14. We created a reconstruction of the pelvis by digitally articulating the Sts 65 right ilium and a mirrored copy of the left ilium with the Sts 14 sacrum in Autodesk Maya. Points along the arcuate line were used to orient the ilia to the sacrum. This reconstruction of the Sts 65 pelvis looks much like a "classic" australopith pelvis, with laterally flared ilia and an inferiorly deflected pubis. An analysis of the obstetric dimensions from our reconstruction shows similarity to other australopiths, a likely transverse or oblique entrance of the neonatal cranium into the pelvic inlet, and a cephalopelvic ratio similar to that found in humans today.

  13. Introduction to the STS National Database Series: Outcomes Analysis, Quality Improvement, and Patient Safety.

    Science.gov (United States)

    Jacobs, Jeffrey P; Shahian, David M; Prager, Richard L; Edwards, Fred H; McDonald, Donna; Han, Jane M; D'Agostino, Richard S; Jacobs, Marshall L; Kozower, Benjamin D; Badhwar, Vinay; Thourani, Vinod H; Gaissert, Henning A; Fernandez, Felix G; Wright, Cam; Fann, James I; Paone, Gaetano; Sanchez, Juan A; Cleveland, Joseph C; Brennan, J Matthew; Dokholyan, Rachel S; O'Brien, Sean M; Peterson, Eric D; Grover, Frederick L; Patterson, G Alexander

    2015-12-01

    The Society of Thoracic Surgeons (STS) National Database is the foundation for most of the Society's quality, research, and patient safety activities. Beginning in January 2016 and repeating each year, The Annals of Thoracic Surgery will publish a monthly Database series of scholarly articles on outcomes analysis, quality improvement, and patient safety. Six articles will be directly derived from the STS National Database and will be published every other month: three articles on outcomes and quality (one each from the STS Adult Cardiac Surgery Database, the STS Congenital Heart Surgery Database, and the STS General Thoracic Surgery Database), and three articles on research (one from each of these three specialty databases). These six articles will alternate with five additional articles on topics related to patient safety. The final article, to be published in December, will provide a summary of the prior 11 manuscripts. This series will allow STS and its Workforces on National Databases, Research Development, and Patient Safety to convey timely information aimed at improving the quality and safety of cardiothoracic surgery. Copyright © 2015 The Society of Thoracic Surgeons. Published by Elsevier Inc. All rights reserved.

  14. Characterization of Volume F Trash from Four Recent STS Missions: Microbial Occurrence, Numbers, and Identifications

    Science.gov (United States)

    Strayer, Richard F.; Hummerick, Mary E.; Richards, Jeffrey T.; McCoy, LaShelle E.; Roberts, Michael S.; Wheeler, Raymond M.

    2011-01-01

    The fate of space-generated solid wastes, including trash, for future missions is under consideration by NASA. Several potential treatment options are under active technology development. Potential fates for space-generated solid wastes: Storage without treatment; storage after treatment(s) including volume reduction, water recovery, sterilization, and recovery plus recycling of waste materials. For this study, a microbial characterization was made on trash returned from four recent STS missions. The material analyzed were 'Volume F' trash and other bags of accompanying trash. This is the second of two submitted papers on these wastes. This first one covered trash content, weight and water content. Upon receipt, usually within 2 days of landing, trash contents were catalogued and placed into categories: drink containers, food waste, personal hygiene items, and packaging materials, i.e., plastic film and duct tape. Microbial counts were obtained with cultivatable counts on agar media and direct counts using Acridine Orange fluorescent stain (AODC). Trash bag surfaces, 25 square cm , were also sampled. Direct counts were approximately 1 x 10(exp 6) microbes/square cm and cultivatable counts ranged from 1 x 10 to 1 X 10(exp 4) microbes/ square cm-2. Aerobic microbes, aerobic sporeformers, and yeasts plus molds were common for all four missions. Waste items from each category were placed into sterile ziplock bags and 1.5 L sterile DI water added. These were then dispersed by hand shaking for 2 min. prior to inoculation of count media or determining AODC. In general, cultivatable microbes were found in drinks, food wastes, and personal hygiene items. Direct counts were usually higher than cultivatable counts. Some pathogens were found: Staphylococcus auerus, Escherichia coli (fecal wastes). Count ranges: drink pouches - AODC 2 x 10(exp 6) to 1 X 10(exp 8) g(sub fw) (exp -1); cultivatable counts variable between missions; food wastes: Direct counts were close to aerobic

  15. Streaming Virtual Reality Content

    OpenAIRE

    El-Ganainy, Tarek; Hefeeda, Mohamed

    2016-01-01

    The recent rise of interest in Virtual Reality (VR) came with the availability of commodity commercial VR prod- ucts, such as the Head Mounted Displays (HMD) created by Oculus and other vendors. To accelerate the user adoption of VR headsets, content providers should focus on producing high quality immersive content for these devices. Similarly, multimedia streaming service providers should enable the means to stream 360 VR content on their platforms. In this study, we try to cover different ...

  16. Content management systems (CMS)

    OpenAIRE

    Oriahi, Harrison

    2014-01-01

    This thesis describes the three most common and widely used content management systems (CMS) used to power several millions of business websites on the internet. Since there are many other content managements systems online, this report provides some helpful guides on each of the three most used systems and the web design projects that each of them maybe most suitable. There are plenty of options when it comes to selecting a content management system for a development project and this the...

  17. Scalable Content Management System

    Directory of Open Access Journals (Sweden)

    Sandeep Krishna S, Jayant Dani

    2013-10-01

    Full Text Available Immense growth in the volume of contents every day demands more scalable system to handle and overcome difficulties in capture, storage, transform, search, sharing and visualization of data, where the data can be a structured or unstructured data of any type. A system to manage the growing contents and overcome the issues and complexity faced using appropriate technologies would advantage over measurable qualities like flexibility, interoperability, customizability, security, auditability, quality, community support, options and cost of licensing. So architecting aContent Management System in terms of enterprise needs and a scalable solution to manage the huge data growth necessitates a Scalable Content Management System.

  18. The role of the IFG and pSTS in syntactic prediction: Evidence from a parametric study of hierarchical structure in fMRI.

    Science.gov (United States)

    Matchin, William; Hammerly, Christopher; Lau, Ellen

    2017-03-01

    Sentences encode hierarchical structural relations among words. Several neuroimaging experiments aiming to localize combinatory operations responsible for creating this structure during sentence comprehension have contrasted short, simple phrases and sentences to unstructured controls. Some of these experiments have revealed activation in the left inferior frontal gyrus (IFG) and posterior superior temporal sulcus (pSTS), associating these regions with basic syntactic combination. However, the wide variability of these effects across studies raises questions about this interpretation. In an fMRI experiment, we provide support for an alternative hypothesis: these regions underlie top-down syntactic predictions that facilitate sentence processing but are not necessary for building syntactic structure. We presented stimuli with three levels of structure: unstructured lists, two-word phrases, and simple, short sentences; and two levels of content: natural stimuli with real words and stimuli with open-class items replaced with pseudowords (jabberwocky). While both the phrase and sentence conditions engaged syntactic combination, our experiment only encouraged syntactic prediction in the sentence condition. We found increased activity for both natural and jabberwocky sentences in the left IFG (pars triangularis and pars orbitalis) and pSTS relative to unstructured word lists and two-word phrases, but we did not find any such effects for two-word phrases relative to unstructured word lists in these areas. Our results are most consistent with the hypothesis that increased activity in IFG and pSTS for basic contrasts of structure reflects syntactic prediction. The pars opercularis of the IFG showed a response profile consistent with verbal working memory. We found incremental effects of structure in the anterior temporal lobe (ATL), and increased activation only for sentences in the angular gyrus (AG)/temporal-parietal junction (TPJ) - both regions showed these effects for

  19. STS-70 Launch - Nikon E-2 Digital Image

    Science.gov (United States)

    1995-01-01

    This test images was taken with a Nikon E-2 Digital Imaging System camera and are provided courtesy of Nikon (GIF 450x450 JPEG 1280x1000): The second Shuttle launch in 16 days hurtles off the pad into a sweltering summer sky. The unstable weather typical to Florida in the summertime didn't have a chance to coalesce and impact this morning's launch window, and the Space Shuttle Discovery began its planned seven-day, 22-hour flight on Mission STS-70 from Launch Pad 39B at 9:41:55.078 a.m. EDT, just seconds off schedule. On board for Discovery's 21st spaceflight are a crew of five: Commander Terence 'Tom' Henricks; Pilot Kevin R. Kregel; and Mission Specialists Nancy Jane Currie, Donald A. Thomas and Mary Ellen Weber. The crew's primary objective during the 70th Shuttle flight is to deploy the Tracking and Data Relay Satellite (TDRS-G), which will join a constellation of other TDRS spacecraft already on orbit. TDRS-G is destined to become an on- orbit, fully operational 'ready reserve' satellite, available along with one other ready reserve TDRS spacecraft to back up the two primary TDRS satellites positions, TDRS East over the Atlantic Ocean and TDRS West over the Pacific. Assured capability of the TDRS communications network is essential for linking Earth-orbiting spacecraft such as the Shuttle and the Hubble Space Telescope with the ground.

  20. A correlative STS: lessons from a Chinese medical practice.

    Science.gov (United States)

    Lin, Wen-Yuan; Law, John

    2014-12-01

    How might Science and Technology Studies learn more from the intersection between 'Western' and 'other' forms of knowledge? In this article, we use Eduardo Viveiros de Castro's writing on equivocal translation to explore a moment of encounter in a Chinese Medical consultation in Taiwan in which a practitioner hybridizes Chinese Medicine and biomedicine. Our description is symmetrical, but creates a descriptive equivocation in which 'Western' analytical terms are used to describe a 'Chinese' medical reality. Drawing on the history of Chinese Medicine, we argue that the latter is not analytical, but 'correlative' in a specifically 'Chinese' manner that explores patternings, flows, and propensities in local collections of things and symptoms. In particular, it both handles difference without seeking to unearth stable causal mechanisms and absorbs new elements including relevant features of biomedicine. We conclude by briefly considering the scope of a possible post-colonial and 'correlative' STS and show that a 'correlative' description of the same Chinese Medical consultation would differ markedly from one making use of 'Western' analytical assumptions.

  1. STS-114: Crew Interviews 1. Andy Thomas 2. Steve Robinson

    Science.gov (United States)

    2005-01-01

    STS-114 Mission Specialists, Andy Thomas and Steve Robinson, are seen in this pre-flight interview. Andy Thomas begins by talking about his interest in spaceflight as a young boy growing up in Australia. He expresses that the chances of an Australian boy studying to eventually become an astronaut was very remote. His Mechanical Engineering Degree in Australia and a Doctorate enabled him to acquire unique skills to come to the United States to work for Lockheed Martin. On the topic of return to flight, he reflects on experiences that he had working with the Michael Anderson and Kalpana Chawla of the ill-fated Space Shuttle Columbia. He also talks about the safety of the Space Shuttle Discovery and repairs to its Thermal Protection system. He explains in detail the Logistics Flight (LF) 1, spacewalks, Multipurpose Logistics Module (MPLM) and the External Stowage Platform (ESP)-2. Steve Robinson expresses that he had many interests as a child and becoming an astronaut was one of them. He was fascinated with things that fly and wanted to find out how they flew. He also designed hang gliders as a teenager. He expresses how his family feels about the risky business of spaceflight. He talks about how the space shuttle discovery crew will remember the Columbia crew by including seven stars on their patch so that they can bring them into orbit and then back home. Robinson also talks about his primary job, and the spacewalks that he and Soichi Noguchi will be performing.

  2. Electrophoretic separation of kidney and pituitary cells on STS-8

    Science.gov (United States)

    Morrison, D. R.; Nachtwey, D. S.; Barlow, G. H.; Cleveland, C.; Lanham, J. W.; Farrington, M. A.; Hatfield, J. M.; Hymer, W. C.; Todd, P.; Wilfinger, W.; Grindeland, R.; Lewis, M. L.

    A Continuous Flow Electrophoresis System (CFES) was used on Space Shuttle flight STS-8 to separate specific secretory cells from suspensions of cultured primary human embryonic kidney cells and rat pituitary cells. The objectives were to isolate the subfractions of kidney cells that produce the largest amounts of urokinase (plasminogen activator), and to isolate the subfractions of rat pituitary cells that secrete growth hormone, prolactin, and other hormones. Kidney cells were separated into more than 32 fractions in each of two electrophoretic runs. Electrophoretic mobility distributions in flight experiments were spread more than the ground controls. Multiple assay methods confirmed that all cultured kidney cell fractions produced some urokinase, and five to six fractions produced significantly more urokinase than the other fractions. Several fractions also produced tissue plasminogen activator. The pituitary cells were separated into 48 fractions in each of the two electrophoretic runs, and the amounts of growth hormone (GH) and prolactin (PRL) released into the medium for each cell fraction were determined. Cell fractions were grouped into eight mobility classes and immunocytochemically assayed for the presence of GH, PRL, ACTH, LH, TSH, and FSH. The patterns of hormone distribution indicate that the specialized cells producing GH and PRL are isolatable due to the differences in electrophoretic mobilities.

  3. STS-40 descent BET products: Development and results

    Science.gov (United States)

    Oakes, Kevin F.; Wood, James S.; Findlay, John T.

    1991-01-01

    Descent Best Estimate Trajectory (BET) Data were generated for the final Orbiter Experiments Flight, STS-40. This report discusses the actual development of these post-flight products: the inertial BET, the Extended BET, and the Aerodynamic BET. Summary results are also included. The inertial BET was determined based on processing Tracking and Data Relay Satellite (TDRSS) coherent Doppler data in conjunction with observations from eleven C-band stations, to include data from the Kwajalein Atoll and the usual California coastal radars, as well as data from five cinetheodolite cameras in the vicinity of the runways at EAFB. The anchor epoch utilized for the trajectory reconstruction was 53,904 Greenwich Mean Time (GMT) seconds which corresponds to an altitude at epoch of approximately 708 kft. Atmospheric data to enable development of an Extended BET for this mission were upsurped from the JSC operational post-flight BET. These data were evaluated based on Space Shuttle-derived considerations as well as model comparisons. The Aerodynamic BET includes configuration information, final mass properties, and both flight-determined and predicted aerodynamic performance estimates. The predicted data were based on the final pre-operational databook, updated to include flight determined incrementals based on an earlier ensemble of flights. Aerodynamic performance comparisons are presented and correlated versus statistical results based on twenty-two previous missions.

  4. STS-133/ET-137 Tanking Test Photogrammetry Assessment

    Science.gov (United States)

    Oliver, Stanley T.

    2012-01-01

    Following the launch scrub of Space Shuttle mission STS-133 on November 5, 2010, an anomalous condition of cracked and raised thermal protection system (TPS) foam was observed on the External Tank (ET). Subsequent dissection of the affected TPS region revealed cracks in the feet of two Intertank (IT) metallic stringers. An extensive investigation into the cause(s) and corrective action(s) for the cracked stringers was initiated, involving a wide array of material and structural tests and nondestructive evaluations, with the intent to culminate into the development of flight rational. One such structural test was the instrumented tanking test performed on December 17, 2010. The tanking test incorporated two three-dimensional optical displacement measurement systems to measure full-field outer surface displacements of the TPS surrounding the affected region that contained the stringer cracks. The results showed that the radial displacement and rotation of the liquid oxygen (LO2) tank flange changed significantly as the fluid level of the LO2 approached and passed the LO2 tank flange.

  5. Gravisensing in flax roots - results from STS-107

    Science.gov (United States)

    Hasenstein, K. H.; Scherp, P.; Ma, Z.

    The goal of the experiment "magnetophoretic induction of curvature in roots" (MICRO) on STS-107 was the induction of curvature in roots by high-gradient magnetic fields (HGMF) in microgravity. The scientific objectives included investigating the growth/curvature pattern in response to a HGMF, the determination of amyloplasts as gravisensing/curvature-inducing structures, and a study of the effects of HGMF and microgravity on the plant cytoskeleton. Flax seeds were germinated in orbit in specially designed seed cassettes. The seeds were oriented so that the emerging roots grew away from the cassette. The magnetic system consisted of ferro-magnetic wedges, magnetized by permanent NdFeB magnets (coercivity > 32k Oe). The HGMF that results from the transition from the high magnetic field density at the wedge tips to air repels diamagnetic amyloplasts. As a result of the previously demonstrated internal displacement of the amyloplasts, the roots were expected to curve as if gravistimulated. Despite successful germination (>90%), the growth rate of the seedlings was significantly lower than comparable controls. Despite the slower growth rate, root curvature was enhanced and initiated earlier than in ground controls. The results indicate that microgravity-grown roots exhibit higher sensitivity for the HGMF than ground controls. The enhanced sensitivity of root curvature in microgravity suggests that the root gravisensing system responds to the displacement of amyloplasts. In the absence of gravity, the higher sensitivity might result from intracellular motion, which in microgravity is likely to be stronger than on the ground.

  6. Unity hatch closed in preparation for launch on STS-88

    Science.gov (United States)

    1998-01-01

    Workers in the Space Station Processing Facility prepare the Unity connecting module for closure before its launch aboard Space Shuttle Endeavour on STS-88 in December. Unity will now undergo a series of leak checks before a final purge of clean, dry air inside the module to ready it for initial operations in space. Other testing includes the common berthing mechanism to which other space station elements will dock and the Pad Demonstration Test to verify the compatibility of the module with the Space Shuttle as well as the ability of the astronauts to send and receive commands to Unity from the flight deck of the orbiter. The next time the hatch will be opened it will be by astronauts on orbit. Unity is expected to be ready for installation into the payload canister on Oct. 25, and transported to Launch Pad 39-A on Oct. 27. The Unity will be mated to the Russian-built Zarya control module which should already be in orbit at that time.

  7. Análisis de varistores mediante STM y STS

    Directory of Open Access Journals (Sweden)

    Castro, M. S.

    2002-02-01

    Full Text Available ZnO-Varistors have been studied by scanning tunnelling microscopy and spectroscopy. Tunnelling current-voltage characteristics indicate that grains present a rectifying behaviour. Since the prebreakdown zone is controlled by Schottky barriers formed by intergranular states, current density vs. temperature curves were fitted considering the tunnelling current through these barriers. Then, the Schottky barrier heights determined using the model are compared with the barrier heights obtained from the scanning tunnelling spectroscopy analysis in the grain boundary.

    Varistores de ZnO fueron estudiados mediante el empleo de microscopía y espectroscopía de efecto túnel. Las curvas de corriente túnel vs. tensión indican que las muestras presentan una conducta rectificante. Dado que la conducción en la zona de pre-ruptura es controlada por la formación de barreras de Schottky en los bordes de grano debidas a la presencia de estados intergranulares, las curvas de densidad de corriente vs. temperatura fueron ajustadas considerando la corriente túnel que atraviesa dichas barreras. La altura de las barreras de Schottky determinada con el modelo es comparada con las alturas obtenidas a través del análisis de barrido por espectroscopía túnel (STS en la región del borde de grano.

  8. Unity hatch closed in preparation for launch on STS-88

    Science.gov (United States)

    1998-01-01

    Workers in the Space Station Processing Facility close the access hatch to the Unity connecting module, part of the International Space Station, before its launch aboard Space Shuttle Endeavour on STS-88 in December. Unity will now undergo a series of leak checks before a final purge of clean, dry air inside the module to ready it for initial operations in space. Other testing includes the common berthing mechanism to which other space station elements will dock and the Pad Demonstration Test to verify the compatibility of the module with the Space Shuttle as well as the ability of the astronauts to send and receive commands to Unity from the flight deck of the orbiter. The next time the hatch will be opened it will be by astronauts on orbit. Unity is expected to be ready for installation into the payload canister on Oct. 25, and transported to Launch Pad 39-A on Oct. 27. The Unity will be mated to the Russian-built Zarya control module which should already be in orbit at that time.

  9. Content-Based Instruction

    Science.gov (United States)

    DelliCarpini, M.; Alonso, O.

    2013-01-01

    DelliCarpini and Alonso's book "Content-Based Instruction" explores different approaches to teaching content-based instruction (CBI) in the English language classroom. They provide a comprehensive overview of how to teach CBI in an easy-to-follow guide that language teachers will find very practical for their own contexts. Topics…

  10. Branded content infantil

    Directory of Open Access Journals (Sweden)

    Raúl RODRÍGUEZ-FERRÁNDIZ

    2017-01-01

    Full Text Available Reseña del libro Bajo la influencia del branded content. Efectos de los contenidos de marca en niños y jóvenes. Review of the book Bajo la influencia del branded content. Efectos de los contenidos de marca en niños y jóvenes.

  11. Content Analysis: Television.

    Science.gov (United States)

    Williams, Tannis MacBeth; And Others

    Content analyses of the depiction of aggression and images of reality on Canadian television were performed on 109 program tapes of top-rated Toronto programs. Content was coded in terms of global messages communicated, character portrayals, context and setting of the program, amount and nature of conflict portrayed, and detailed information on…

  12. Print advertising : Vivid content

    NARCIS (Netherlands)

    Fennis, B.M.; Das, E.H.H.J.; Fransen, M.L.

    2012-01-01

    The present research examines the effects of vivid ad content in two types of appeal in print ads as a function of individual differences in chronically experienced vividness of visual imagery. For informational ads for a functional product, vivid ad content strongly affected individuals high in rep

  13. Print advertising: vivid content

    NARCIS (Netherlands)

    Fennis, B.M.; Das, E.; Fransen, M.L.

    2012-01-01

    The present research examines the effects of vivid ad content in two types of appeal in print ads as a function of individual differences in chronically experienced vividness of visual imagery. For informational ads for a functional product, vivid ad content strongly affected individuals high in rep

  14. Hydroponics: Content and Rationale

    Science.gov (United States)

    Ernst, Jeremy V.; Busby, Joe R.

    2009-01-01

    Technology education has the means of becoming the catalyst for integrated content and curricula, especially in core academic areas, such as science and mathematics, where it has been found difficult to incorporate other subject matter. Technology is diverse enough in nature that it can be addressed by a variety of content areas, serving as a true…

  15. Content-Based Instruction

    Science.gov (United States)

    DelliCarpini, M.; Alonso, O.

    2013-01-01

    DelliCarpini and Alonso's book "Content-Based Instruction" explores different approaches to teaching content-based instruction (CBI) in the English language classroom. They provide a comprehensive overview of how to teach CBI in an easy-to-follow guide that language teachers will find very practical for their own contexts. Topics…

  16. Hydroponics: Content and Rationale

    Science.gov (United States)

    Ernst, Jeremy V.; Busby, Joe R.

    2009-01-01

    Technology education has the means of becoming the catalyst for integrated content and curricula, especially in core academic areas, such as science and mathematics, where it has been found difficult to incorporate other subject matter. Technology is diverse enough in nature that it can be addressed by a variety of content areas, serving as a true…

  17. Social video content delivery

    CERN Document Server

    Wang, Zhi; Zhu, Wenwu

    2016-01-01

    This brief presents new architecture and strategies for distribution of social video content. A primary framework for socially-aware video delivery and a thorough overview of the possible approaches is provided. The book identifies the unique characteristics of socially-aware video access and social content propagation, revealing the design and integration of individual modules that are aimed at enhancing user experience in the social network context. The change in video content generation, propagation, and consumption for online social networks, has significantly challenged the traditional video delivery paradigm. Given the massive amount of user-generated content shared in online social networks, users are now engaged as active participants in the social ecosystem rather than as passive receivers of media content. This revolution is being driven further by the deep penetration of 3G/4G wireless networks and smart mobile devices that are seamlessly integrated with online social networking and media-sharing s...

  18. The STS-93 crew takes part in payload familiarization of the Chandra X-ray Observatory

    Science.gov (United States)

    1999-01-01

    A TRW technician joins STS-93 Commander Eileen Collins (center) and Pilot Jeffrey S. Ashby (right) as they observe the Chandra X- ray Observatory on its work stand inside the Vertical Processing Facility. Other members of the STS-93 crew who are at KSC for payload familiarization are Mission Specialists Catherine G. Coleman and Michel Tognini of France, who represents the Centre National d'Etudes Spatiales (CNES). Collins is the first woman to serve as a shuttle mission commander. She was the first woman pilot of a Space Shuttle, on mission STS-63, and also served as pilot on mission STS-84. The fifth member of the crew is Mission Specialist Steven A. Hawley. Chandra is scheduled for launch July 9 aboard Space Shuttle Columbia, on mission STS-93 . Formerly called the Advanced X-ray Astrophysics Facility, Chandra comprises three major elements: the spacecraft, the science instrument module (SIM), and the world's most powerful X-ray telescope. Chandra will allow scientists from around the world to see previously invisible black holes and high-temperature gas clouds, giving the observatory the potential to rewrite the books on the structure and evolution of our universe.

  19. A comparison of carbon depletion on STS-8 with atmospheric atomic oxygen flux

    Energy Technology Data Exchange (ETDEWEB)

    Triolo, J. [Swales and Associates Inc., 5050 Powder Mill Road, Beltsville, Maryland 20705 (United States); Kruger, R. [Technical Services, 4740 Connecticut Ave. N. W., Washington, District of Columbia 20008 (United States); Chen, P.; Straka, S. [NASA Goddard Space Flight Center, Greenbelt, Maryland 20771 (United States)

    1996-03-01

    In the early and mid 1980s, there were a number of experiments flown aboard the Space Transportation System (STS) Shuttle to measure contamination accumulation and atomic oxygen erosion effects on various materials. One of these experiments was the Contamination Monitoring Package (CMP), designed and built at the National Aeronautics and Space Administration (NASA) Goddard Space Flight Center (GSFC). The CMP was a small, easily integrated instrument which basically consisted of a box with four Temperature Controlled Quartz Crystal Microbalances (TQCMs), a tape recorder, and electronics. The CMP flew on several of the early Shuttle missions including STS-3, 8, and 11. The focus of this paper is to present the results of the CMP experiment flown on the STS-8 mission. This CMP mission was designed to measure atomic oxygen erosion of several different materials, including erosion of carbon from a TQCM. This paper presents the data and discusses the results from the STS-8 CMP experiment and seeks to establish a correlation model between predicted atomic oxygen densities and the carbon erosion rates observed during the STS-8 mission. {copyright} {ital 1996 American Institute of Physics.}

  20. STS-111 M.S. Chang-Diaz suits up for launch

    Science.gov (United States)

    2002-01-01

    KENNEDY SPACE CENTER, FLA. -- STS-111 Mission Specialist Franklin Chang-Diaz suits up again for the second launch attempt aboard Space Shuttle Endeavour on mission STS-111 to the International Space Station. This mission marks the 14th Shuttle flight to the Space Station and the third Shuttle mission this year. Mission STS-111 is the 18th flight of Endeavour and the 110th flight overall in NASA's Space Shuttle program. On mission STS-111, astronauts will deliver the Leonardo Multi-Purpose Logistics Module, the Mobile Base System (MBS), and the Expedition Five crew to the Space Station. During the seven days Endeavour will be docked to the Station, three spacewalks will be performed dedicated to installing MBS and the replacement wrist-roll joint on the Station's Canadarm2 robotic arm. Endeavour will also carry the Expedition 5 crew, who will replace Expedition 4 on board the Station. Expedition 4 crew members will return to Earth with the STS-111 crew. Liftoff is scheduled for 5:22 p.m. EDT from Launch Pad 39A.