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Sample records for juan fernandez microplate

  1. Botanical and Geological Significance of Potassium-Argon Dates from the Juan Fernandez Islands

    Science.gov (United States)

    Stuessy, Tod F.; Foland, K. A.; Sutter, John F.; Sanders, Roger W.; Silva O., Mario

    1984-07-01

    Potassium-argon dating of five basalts from the three main islands of the Juan Fernandez (or Robinson Crusoe) Islands of Chile in the southeastern Pacific gives ages of 1.01 ± 0.12 and 2.44 ± 0.14 million years for Masafuera 3.79 ± 0.20 and 4.23 ± 0.16 for Masatierra, and 5.8 ± 2.1 for Santa Clara. These ages are much younger than that of the underlying oceanic plate and are consistent with the origin of the island-seamount chain from a mantle hot spot beneath the eastward moving Nazca plate. The young age for the archipelago suggests that speciation within endemic genera has occurred within the past 4 to 5 million years. Endemic genera of apparently more ancient origins, such as Lactoris and Thyrsopteris, have apparently dispersed to the islands and survive refugially.

  2. Marine Biodiversity in Juan Fernandez and Desventuradas Islands, Chile: Global Endemism Hotspots.

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    Alan M Friedlander

    Full Text Available The Juan Fernández and Desventuradas islands are among the few oceanic islands belonging to Chile. They possess a unique mix of tropical, subtropical, and temperate marine species, and although close to continental South America, elements of the biota have greater affinities with the central and south Pacific owing to the Humboldt Current, which creates a strong biogeographic barrier between these islands and the continent. The Juan Fernández Archipelago has ~700 people, with the major industry being the fishery for the endemic lobster, Jasus frontalis. The Desventuradas Islands are uninhabited except for a small Chilean military garrison on San Félix Island. We compared the marine biodiversity of these islands across multiple taxonomic groups. At San Ambrosio Island (SA, in Desventuradas, the laminarian kelp (Eisenia cokeri, which is limited to Desventuradas in Chile, accounted for >50% of the benthic cover at wave exposed areas, while more sheltered sites were dominated by sea urchin barrens. The benthos at Robinson Crusoe Island (RC, in the Juan Fernández Archipelago, comprised a diverse mix of macroalgae and invertebrates, a number of which are endemic to the region. The biomass of commercially targeted fishes was >2 times higher in remote sites around RC compared to sheltered locations closest to port, and overall biomass was 35% higher around SA compared to RC, likely reflecting fishing effects around RC. The number of endemic fish species was extremely high at both islands, with 87.5% of the species surveyed at RC and 72% at SA consisting of regional endemics. Remarkably, endemics accounted for 99% of the numerical abundance of fishes surveyed at RC and 96% at SA, which is the highest assemblage-level endemism known for any individual marine ecosystem on earth. Our results highlight the uniqueness and global significance of these biodiversity hotspots exposed to very different fishing pressures.

  3. A double seismic zone in the subducting Juan Fernandez Ridge of the Nazca Plate (32°S), central Chile

    Science.gov (United States)

    Marot, M.; Monfret, T.; Pardo, M.; Ranalli, G.; Nolet, G.

    2013-07-01

    The region of central Chile offers a unique opportunity to study the links between the subducting Juan Fernandez Ridge, the flat slab, the double seismic zone (DSZ), and the absence of modern volcanism. Here we report the presence and characteristics of the first observed DSZ within the intermediate-depth Nazca slab using two temporary seismic catalogs (Ovalle 1999 and Chile Argentina Seismological Measurement Experiment). The lower plane of seismicity (LP) is located 20-25 km below the upper plane, begins at 50 km depth, and merges with the lower plane at 120 km depth, where the slab becomes horizontal. Focal mechanism analysis and stress tensor calculations indicate that the slab's state of stress is dominantly controlled by plate convergence and overriding crust thickness: Above 60-70 km depth, the slab is in horizontal compression, and below, it is in horizontal extension, parallel to plate convergence, which can be accounted for by vertical loading of the overriding lithosphere. Focal mechanisms below 60-70 km depth are strongly correlated with offshore outer rise bend faults, suggesting the reactivation of preexisting faults below this depth. The large interplane distances for all Nazca DSZs can be related to the slab's unusually cold thermal structure with respect to its age. Since LPs globally seem to mimic mantle mineral dehydration paths, we suggest that fluid migration and dehydration embrittlement provide the mechanism necessary to weaken the rock and that the stress field determines the direction of rupture.

  4. Is the Juan Fernandez Ridge (nazca Plate) a Deep-Mantle Hot SPOT Trail?

    Science.gov (United States)

    Lara, L. E.; Selles, D.; Díaz, A.; Piña-Gauthier, M.

    2011-12-01

    The Juan Fernández Ridge on the oceanic Nazca plate is thought to be a classic hot spot trail because of the apparent westward rejuvenation of the eruptive ages. Geochronological data is still scarce to prove this is the case, and other hypothesis should be taken into account. There are a few constrains, like the ca. 9 Ma Ar-Ar age of the O'Higgins seamount (115 km from the Chile-Perú trench), published K-Ar ages of ca. 3-4 Ma in Robinson Crusoe island (580 km from the trench) and ca. 1 Ma in Alejandro Selkirk (180 km further west). New reconnaissance K-Ar ages in Robinson Crusoe yield ca. 1-3 Ma, which partially overlap with the age of Alejandro Selkirk, breaking the expected age progression given that the Nazca plate moves eastwards at ca. 6-8 cm/yr. New geological mapping also shows a sharp unconformity between the older, strongly altered sequences and more recent, post-erosional volcanic piles, where only the vent facies have disappeared. A fixed deep-mantle plume origin for Pacific hot spots has been widely debated and concurrent phenomena arose as a possible explanation for non-linear age progressions and/or long-lived volcanic activity. In fact, intraplate regional tectonics, plume displacement, and mantle heterogeneities could be the main factor of the ridge architecture or the mask for a first-order linear trend. An ongoing mapping and dating effort is aimed to understand the evolution of the Juan Fernández Ridge, testing the main hypothesis. Fondecyt grant 110966 is acknowledged for financial support.

  5. The Teatro Guindalera Project. An Interview with Juan Pastor MilletFernandez

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    Mª-Yolanda Fernández-Suárez

    2011-03-01

    Full Text Available Juan Pastor Millet was born in Alicante in 1943. He has a degree in Drama and did his training with William Layton and Arnold Taraborrelli in Laboratorio T.E.I. Till 1980 he worked as a theatre, cinema and TV actor. As an independent director he has produced plays by both Spanish and foreign contemporary authors, and also by classic ones like Calderón de la Barca, Shakespeare, Cervantes, Molière, Ibsen, Strindberg and Brecht. From 1987 till 2006 he combined his job as a director with his job as a Drama teacher at RESAD (Real Escuela Superior de Arte Dramático. In November 2003 he opened his own Studio and Theatre House in Madrid – Guindalera. From then on he devotes all his time to the family project Guindalera Escena Abierta  together with his wife Teresa Valentín-Gamazo and their daughter, the actress María Pastor. In 2009 while they were celebrating Brian Friel’s 80th anniversary staging three of his plays – El Juego de Yalta, Molly Sweeney and Bailando en Lughnasa – they were awarded the prestigious “Premio Ojo Crítico de Teatro” for their commitment to new authors and their pedagogical character.

  6. Applying Fishers' ecological knowledge to construct past and future lobster stocks in the Juan Fernandez Archipelago, Chile.

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    Tyler D Eddy

    Full Text Available Over-exploited fisheries are a common feature of the modern world and a range of solutions including area closures (marine reserves; MRs, effort reduction, gear changes, ecosystem-based management, incentives and co-management have been suggested as techniques to rebuild over-fished populations. Historic accounts of lobster (Jasus frontalis on the Chilean Juan Fernández Archipelago indicate a high abundance at all depths (intertidal to approximately 165 m, but presently lobsters are found almost exclusively in deeper regions of their natural distribution. Fishers' ecological knowledge (FEK tells a story of serial depletion in lobster abundance at fishing grounds located closest to the fishing port with an associated decline in catch per unit effort (CPUE throughout recent history. We have re-constructed baselines of lobster biomass throughout human history on the archipelago using historic data, the fishery catch record and FEK to permit examination of the potential effects of MRs, effort reduction and co-management (stewardship of catch to restore stocks. We employed a bioeconomic model using FEK, fishery catch and effort data, underwater survey information, predicted population growth and response to MR protection (no-take to explore different management strategies and their trade-offs to restore stocks and improve catches. Our findings indicate that increased stewardship of catch coupled with 30% area closure (MR provides the best option to reconstruct historic baselines. Based on model predictions, continued exploitation under the current management scheme is highly influenced by annual fluctuations and unsustainable. We propose a community-based co-management program to implement a MR in order to rebuild the lobster population while also providing conservation protection for marine species endemic to the Archipelago.

  7. Aerobic and anaerobic enzymatic activity of orange roughy (Hoplostethus atlanticus) and alfonsino (Beryx splendens) from the Juan Fernandez seamounts area.

    Science.gov (United States)

    Saavedra, L M; Quiñones, R A; Gonzalez-Saldía, R R; Niklitschek, E J

    2016-06-01

    The aerobic and anaerobic enzymatic activity of two important commercial bathypelagic species living in the Juan Fernández seamounts was analyzed: alfonsino (Beryx splendens) and orange roughy (Hoplostethus atlanticus). These seamounts are influenced by the presence of an oxygen minimum zone (OMZ) located between 160 and 250 m depth. Both species have vertical segregation; alfonsino is able to stay in the OMZ, while orange roughy remains at greater depths. In this study, we compare the aerobic and anaerobic capacity of these species, measuring the activity of key metabolic enzymes in different body tissues (muscle, heart, brain and liver). Alfonsino has higher anaerobic potential in its white muscle due to greater lactate dehydrogenase (LDH) activity (190.2 μmol NADH min(-1) g ww(-1)), which is related to its smaller body size, but it is also a feature shared with species that migrate through OMZs. This potential and the higher muscle citrate synthase and electron transport system activities indicate that alfonsino has greater swimming activity level than orange roughy. This species has also a high MDH/LDH ratio in its heart, brain and liver, revealing a potential capacity to conduct aerobic metabolism in these organs under prolonged periods of environmental low oxygen conditions, preventing lactic acid accumulation. With these metabolic characteristics, alfonsino may have increased swimming activity to migrate and also could stay for a period of time in the OMZ. The observed differences between alfonsino and orange roughy with respect to their aerobic and anaerobic enzymatic activity are consistent with their characteristic vertical distributions and feeding behaviors.

  8. Seamounts in the southeastern Pacific Ocean and biodiversity on Juan Fernandez seamounts, Chile Montes submarinos en el océano Pacífico suroriental y biodiversidad en el cordón de Juan Fernández, Chile

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    Eleuterio Yáñez

    2009-01-01

    Full Text Available Seamounts are vulnerable marine ecosystems. In Chile, information on these ecosystems is quite scarce; thus, a compilation of information on their geographical distribution and biodiversity is presented herein. A total of 118 seamounts distributed in the Chilean EEZ are identified and characterized. Additionally, an in situ assessment was carried out on the Juan Fernandez seamounts 1 and 2 (JF1 and JF2, which were also oceanographically characterized. Phytoplankton, zooplankton, and marine invertebrate samples were collected and an exploratory fishing survey was executed using different gears. According to the bibliographical review, a total of 82 species have been collected on the JF1 and JF2 seamounts, highlighting fmdings of black coral species caught in lobster traps at the Juan Fernandez Archipelago. Submarine images of the marine substrate at JF1 and JF2 reveal characteristics attributable to the impact of bottom dredges, coinciding with the information obtained from the trawling fleet. The fishing activity was carried out primarily at JF2 (4,667 km of trawling. The monthly fishing effort increased considerably in 2002, 2003, and 2005, reaching values above 500 km of trawling and, thus, modifying the spatial structure of the resource aggregates on the JF2 seamount.Los montes submarinos constituyen ecosistemas marinos vulnerables. Chile presenta una escasa información acerca de estos ecosistemas, por lo que este trabajo recopila información sobre distribución geográfica y biodiversidad. Se identifican y caracterizan 118 montes en la ZEE de Chile. Adicionalmente, una evaluación in situ se desarrolló sobre los montes Juan Fernández 1 y 2 (JF1, JF2, caracterizándolos oceanógraficamente. Se recolectaron muestras de fitoplancton, zooplancton e invertebrados marinos, y se realizó pesca exploratoria con diversos artes. La revisión bibliográfica establece que en JF1 y JF2, se han capturado un total de 82 especies, destacándose la

  9. Ciclo anual de la clorofila-α satelital en el archipiélago de Juan Fernández (33°S, Chile Annual cycle of the satellite chlorophyll-a in the Juan Fernandez archipelago (33°S, Chile

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    Isabel Andrade

    2012-09-01

    Full Text Available La variabilidad espacial y temporal del ciclo anual de la clorofila-α en el archipiélago de Juan Fernández se analiza usando ocho anos de datos satelitales de clorofila-α, viento, corrientes y nivel del mar. Este archipiélago está conformado por tres islas: Robinson Crusoe-Santa Clara (RC-SC y Alejandro Selkirk (AS. Las islas RC-SC se encuentran en una región de alta energía cinética y mayores concentraciones de clorofila-α definida como Zona de Transición Costera, en cambio la isla AS se encuentra en una región de baja energía cinética y menores concentraciones de clorofila-α denominada Zona Oceánica. RC-SC posee un incremento de las concentraciones de clorofila-a en el lado suroeste, mientras que AS posee sus mayores concentraciones en el lado oeste, indicando la presencia de procesos forzantes diferentes. En ambas islas el ciclo anual de la clorofila es significativo y comienza a incrementar en abril, alcanzando valores relativamente altos entre junio y noviembre, disminuyendo hacia diciembre, hasta alcanzar un mínimo hacia fines de marzo. Este ciclo anual aparece desacoplado con el viento y acoplado con la energía cinética. Incrementos invernales de clorofila-a aparecen vinculados con remolinos de mesoescala provenientes de la zona continental, mientras que incrementos primaverales se podrían asociar a procesos locales vinculados al "efecto masa de isla". En ambas islas existen incrementos significativos de la clorofila-a en la banda anual y cuasi-bianual, pero sólo en AS se encontró una fluctuación significativa en la banda interanual vinculada con el Nino y la Oscilación del Sur.The spatial and temporal variability of the annual cycle of chlorophyll-a in the Juan Fernandez archipelago is analyzed through eight years of satellite data of chlorophyll-a, wind, currents and sea level. This archipelago consists of three islands: Robinson Crusoe-Santa Clara (RC-SC and Alexander Selkirk (AS. The RC-SC islands are in a

  10. Dynamics of Microplates

    Science.gov (United States)

    Bodenschatz, E.; Katz, R.

    2005-12-01

    Rotating, growing microplates are observed in a wax analog model of sea-floor spreading. We show that wax microplates are kinematically similar to sea-floor tectonic microplates in terms of spreading rate, growth rate and their characteristic spiral pseudofault geometry. We propose a theory for the formation of microplates.

  11. El teatro de Fernandez de Lizardi

    NARCIS (Netherlands)

    Raffi-Béroud, Catherine Marie Hélene

    1994-01-01

    Tot op heden hebben de specialisten op het gebied van de Latijsamerikaanse literatuur, en vooral de Mexicanen, in hoofdzaak interesse getoond voor de eerste roman van Jose Joaquin Fernandez de Lizardi (1776-1827), Periquillo Sarniento (1816). Zijn andere drie romans, evenals zijn poezie en zijn

  12. Rendimientos, estructuras de tallas y madurez sexual del alfonsino (Beryx splendens capturado en el cordón submarino de Juan Fernández, Chile Fishing yields, size structures, and sexual maturity of alfonsino (Beryx splendens caught on Juan Fernandez seamounts, Chile

    Directory of Open Access Journals (Sweden)

    Aurora Guerrero

    2009-01-01

    Full Text Available Se analiza la información recopilada en lances comerciales para la captura de alfonsino (Beryx splendens realizados en montes submarinos del archipiélago de Juan Fernández (Chile, entre los años 2001 y 2003, que corresponden a la etapa de desarrollo inicial de esta pesquería. En dicho período se efectuaron 187 lances, de los cuales en 121 se obtuvo la especie objetivo, capturándose 525,1 ton, que constituyeron el 99,2% de la captura total. Por la configuración irregular de los fondos, los lances de pesca se caracterizaron por su corta duración (0,26-0,50 h, a profundidad media de 469,7 m. En este periodo se analizaron 14.773 ejemplares, 6.064 machos (41% y 8.709 hembras (59%, evidenciando un predominio de hembras. La proporción sexual estructurada a la talla mostró un predominio de machos a tallas inferiores a 24 cm de longitud de horquilla (LH. En machos el tamaño medio fluctuó entre 34,6 y 36,8 cm de LH y en hembras entre 36,2 y 38,4 cm LH. Para determinar la madurez de las gónadas, se utilizó la escala macroscópica propuesta por Lehodey et al. (1997 y se estableció que los machos alcanzaron la primera madurez (TMS50% a 34,3 cm LH y las hembras a los 33,3 cm LH. Los rendimientos promedio en el período analizado correspondieron entre 0,2 y 6,6 ton lance-1 y entre 1,8 y 19,0 ton h.a.-1 , con medias de 4,3 ton lance-1 y 9,2 ton h.a.-1 .We analyzed information collected during commercial hauls targeting alfonsino (Beryx splendens on seamounts of the Juan Fernández Archipelago (Chile from the early stages of the fishery (2001-2003. Of the 187 hauls carried out in this period, 121 were successful; alfonsino catches reached 525.1 ton, constituting 99.2%> of the total catch. Due to the irregularity of the sea bottom, short (0.26-0.50 h fishing hauls were performed at mid-depth (469.7 m. We analyzed 14,773 specimens during the study period: 6,064 males (41% and 8,709 females (59%, the latter being predominan! The size structure was

  13. Microplate Heat Exchanger Project

    Data.gov (United States)

    National Aeronautics and Space Administration — We propose a microplate heat exchanger for cryogenic cooling systems used for continuous flow distributed cooling systems, large focal plane arrays, multiple cooling...

  14. Tectonic microplates: laying it down on wax

    Science.gov (United States)

    Katz, R. R.; Bodenschatz, E.

    2008-12-01

    We present a wax analogue model of sea-floor spreading that produces rotating, growing microplates. Wax microplates are kinematically similar to sea-floor tectonic microplates in terms of spreading rate and growth rate. Furthermore, their spiral pseudofault geometry is quantitatively consistent with Schouten's oceanic microplate model. These results suggest that Schouten's edge-driven microplate model captures the kinematics of tectonic microplate evolution on Earth. We propose a theory for the formation of microplates.

  15. 24 June 2014 - Latin America Ambassadors to Switzerland visiting the LHC tunnel at Point 1 with Beams Department R. A. Fernandez

    CERN Multimedia

    Egli, Laurent

    2014-01-01

    Visitors present in random order: Ambassador Antonio Gustavo Trombetta (Argentina) ; Ambassador Igor Kipman (Brasil) ; Ambassador Beatriz Londoño Soto (Colombia) ; Ambassador Isabel Montero de la Cámara (Costa Rica) ; Ambassador María del Pilar Fernandez Otero (Cuba) ; Ambassador Jorge Castro-Valle Kuehne (México) ; Researcher Luis Roberto Flores Castillo (México) ; Chargé d’affaires Esteban Paez Gonzalez (Paraguay) ; Ambassador Luis Juan Chuquihuara Chil (Perú) ; Ambassador Cesar Osvelio Méndez (Venezuela)

  16. Transparency microplates under impact.

    Science.gov (United States)

    Lau, Chun Yat; Roslan, Zulhanif; Cheong, Brandon Huey-Ping; Chua, Wei Seong; Liew, Oi Wah; Ng, Tuck Wah

    2014-07-15

    Transparency microplates enable biochemical analysis in resource-limited laboratories. During the process of transfer, the analytes tittered into the wells may undergo spillage from one well to another due to lateral impact. Sidelong impact tests conducted found the absence of non-linear effects (e.g., viscoelastic behavior) but high energy loss. Finite element simulations conducted showed that the rectangular plate holding the transparencies could undergo z-axis deflections when a normal component of the force was present despite constraints being used. High speed camera sequences confirmed this and also showed the asymmetrical z-axis deflection to cause the contact line closer to impact to displace first when the advancing condition was exceeded. Capillary waves were found to travel toward the contact line at the opposite end, where if the advancing contact angle condition was exceeded, also resulted in spreading. The presence of surface scribing was found to limit contact line movement better. With water drops dispensed on scribed transparencies, immunity from momentum change of up to 9.07 kgm/s on impact was possible for volumes of 40 μL. In the case of glycerol drops immunity from momentum change of up to 9.07 kgm/s on impact extended to volumes of 90 μL. The improved immunity of glycerol was attributed to its heightened dampening characteristics and its higher attenuation of capillary waves. Overall, scribed transparency microplates were able to better withstand spillage from accidental impact. Accidental impact was also found not to cause any detrimental effects on the fluorescence properties of enhanced green fluorescent protein samples tested.

  17. Edge-driven microplate kinematics

    Science.gov (United States)

    Schouten, Hans; Klitgord, Kim D.; Gallo, David G.

    1993-01-01

    It is known from plate tectonic reconstructions that oceanic microplates undergo rapid rotation about a vertical axis and that the instantaneous rotation axes describing the microplate's motion relative to the bounding major plates are frequently located close to its margins with those plates, close to the tips of propagating rifts. We propose a class of edge-driven block models to illustrate how slip across the microplate margins, block rotation, and propagation of rifting may be related to the relative motion of the plates on either side. An important feature of these edge-driven models is that the instantaneous rotation axes are always located on the margins between block and two bounding plates. According to those models the pseudofaults or traces of disrupted seafloor resulting from the propagation of rifting between microplate and major plates may be used independently to approximately trace the continuous kinematic evolution of the microplate back in time. Pseudofault geometries and matching rotations of the Easter microplate show that for most of its 5 m.y. history, block rotation could be driven by the drag of the Nazca and Pacific plates on the microplate's edges rather than by a shear flow of mantle underneath.

  18. Aphoristic Compendium. A tribute to Juan Magariños. Óscar Fernandez Galindez

    OpenAIRE

    Álvarez, Martha Elena; Universidad de Medellín

    2013-01-01

    Óscar José Fernández, biólogo, poeta y pensador de la complejidad, en su libro Compendio aforístico, trata de explicar desde una mirada realmente integradora y metacompleja –la biología filosófica2– asuntos tan distantes, para algunos, -como ciencia, cultura, ética, sociedad, biología, ecología, lógica, tecnología, filosofía, arte, teología-, pero, a su vez, tan entrelazados en la existencia humana de una manera sucinta, poética y trandisciplinar. Esta obra ofrece, en un lenguaje aforístico, ...

  19. Kinematics of the Danakil microplate

    OpenAIRE

    2002-01-01

    A refinement and extrapolation of recent motion estimates for the Danakilmicroplate, based on ancient kinematic indicators in the Afar region, describesthe evolution of a microplate in the continental realm. The Danakil horst is anelevated part of this microplate, exposing Precambrian basement within theAfar depression, site of the Nubia-Somalia-Arabia triple junction. We compareevidence for strike- or oblique-slip faults in data from the Afar depression andsouthern Red Sea to small circles a...

  20. Variation in the mitochondrial control region in the Juan Fernández fur seal (Arctocephalus philippii).

    Science.gov (United States)

    Goldsworthy, S; Francis, J; Boness, D; Fleischer, R

    2000-01-01

    The Juan Fernandez fur seal (Arctocephalus philippii was allegedly extremely abundant, numbering as many as 4 million prior to sealing which continued from the late 17th to the late 19th century. By the end of the sealing era the species was thought to be extinct until they were rediscovered at Alejandro Selkirk Island in 1965. Historic records would suggest that the species underwent a substantial population bottleneck as a result of commercial sealing, and from population genetic theory we predicted that the genetic variability in the species would be low. We compared the mtDNA control region sequence from 28 Juan Fernandez fur seals from two islands in the Juan Fernandez Archipelago (Chile). Contrary to expectation, we found that variation in the Juan Fernandez fur seals is not greatly reduced in comparison to other pinniped taxa, especially given the apparent severity of the bottleneck they underwent. We also determined minor, but significantly different haplotype frequencies among the populations on the two islands (Alejandro Selkirk and Robinson Crusoe Islands), but no difference in their levels of variability. Such differences may have arisen stochastically via a recent founder event from Alejandro Selkirk to Robinson Crusoe Island or subsequent genetic drift.

  1. Kinematics of the Danakil microplate

    Science.gov (United States)

    Eagles, Graeme; Gloaguen, Richard; Ebinger, Cynthia

    2002-10-01

    A refinement and extrapolation of recent motion estimates for the Danakil microplate, based on ancient kinematic indicators in the Afar region, describes the evolution of a microplate in the continental realm. The Danakil horst is an elevated part of this microplate, exposing a Precambrian basement within the Afar depression, the site of the Nubia-Somalia-Arabia triple junction. We compare evidence for strike- or oblique-slip faults in data from the Afar depression and southern Red Sea to small circles about published poles of rotation for the Danakil microplate with respect to Nubia. A reconstruction about the preferred pole reunites lengths of a Precambrian shear zone on the Nubia and Danakil sides and preserves a uniform basement fabric strike through Nubia, Danakil and Yemen. Since at least magnetic chron C5 (˜11 Ma) Danakil rotated about a different pole with respect to Nubia than either Somalia or Arabia, but between chrons C5 and C2A Nubia-Danakil motion was a close approximation to Nubia-Somalia motion. Since C2A relative motions of the Danakil microplate have been independent of movements on any of the neighbouring plate boundaries. We relate this to the onset of oceanic-type accretion within Afar. The resulting eastwards acceleration of Danakil was accommodated by westwards propagation of the Gulf of Aden rift that became the new, discrete, plate boundary between the Danakil microplate and the Somalia plate. Present-day activity suggests that the Red Sea and Aden rifts will link through Afar, thereby isolating the Danakil horst as a microcontinent on the Arabian margin.

  2. Cristina Fernandez de Kirchner. Argentina esileedi sihib abikaasa kohta / Evelyn Kaldoja

    Index Scriptorium Estoniae

    Kaldoja, Evelyn, 1980-

    2007-01-01

    Ilmunud ka: Postimees : na russkom jazõke 30. okt. lk. 11. Argentina presidendivalimiste kindlaks võitjaks on ajakirjanduse arvates praeguse presidendi abikaasa Cristina Fernandez de Kirchner. Vt. samas: Presidendiproua lähim konkurent on endine iluduskuninganna

  3. Cristina Fernandez de Kirchner. Argentina esileedi sihib abikaasa kohta / Evelyn Kaldoja

    Index Scriptorium Estoniae

    Kaldoja, Evelyn, 1980-

    2007-01-01

    Ilmunud ka: Postimees : na russkom jazõke 30. okt. lk. 11. Argentina presidendivalimiste kindlaks võitjaks on ajakirjanduse arvates praeguse presidendi abikaasa Cristina Fernandez de Kirchner. Vt. samas: Presidendiproua lähim konkurent on endine iluduskuninganna

  4. Real Plates and Dubious Microplates

    Science.gov (United States)

    Kogan, M. G.; Steblov, G. M.

    2008-12-01

    From the onset of plate tectonics, the existence of most of the plates was never put in doubt, although the boundaries of some plates, like Africa, were later revised. There are however, two microplates in northeast Asia, the Amurian and Okhotsk, whose existence and the sense of rotation was revised several times. The rms value of plate-residual GPS velocities is 0.5-0.9 mm/a for sets of stations representing the motion of the following plates: Antarctic, Australian, Eurasian, North American, Nubian, Pacific, and South American. This value can be regarded as an upper bound on deviation of real plates from infinite stiffness. The rms value of plate-residual GPS velocities is 1.2-1.8 mm/a for the Indian, Nazca, and Somalian plates. Higher rms values for India and Nazca are attributed to the noisier data. The higher rms value for Somalia appears to arise from the distributed deformation to the east of the East African Rift; whether this statement is true can only be decided from observations of denser network in the future. From the analysis of plate-residual GPS velocities, the Canadian Arctic and northeastern Siberia belong to the North American plate. The detailed GPS survey on Sakhalin Island shows that the Sea of Okhotsk region also belongs to the North American plate while the region to the west of it belongs to the Eurasian plate. These results provide a constraint on the geometry of the North American plate and put in doubt the existence of smaller plates in northeast Asia.

  5. Versatile wetting measurement of microplate wells

    Science.gov (United States)

    Ng, Enoch Ming Wei; Cheong, Brandon Huey-Ping; Yu, Yang; Liew, Oi Wah; Ng, Tuck Wah

    2016-11-01

    A method to measure the contact angle, which is indicative of wetting, using small liquid volumes dispensed directly on microplate wells is described and demonstrated. Experiments with enhanced green protein samples of volumes 4.4-6 μl showed no measured variance in the contact angle. Experiments with phosphate buffer solution with varied concentrations of a non-ionic detergent (Tween 20) dissolved, however, revealed smaller contact angles with increased detergent concentration. It is experimentally shown that drops can be located up to 7° from the lowest position of the well without affecting the accuracy of contact angle measurements. Numerical simulations confirm the ability of the drops to manifest the correct contact angle despite the lack of axis-symmetry in their shape while residing on a circular surface. This method offers a convenient means to determine the wetting characteristics of different liquid samples in different microplates.

  6. Entrevista: Juan Pablo Gramsch

    Directory of Open Access Journals (Sweden)

    Juan Pablo Gramsch

    2015-04-01

    A cargo de este Programa, se desempeña prácticamente desde sus inicios como Coordinador General el Arquitecto señor Juan Pablo Gramsch Labra, titulado en la Pontificia Universidad Católica de Chile y con estudios de Post Grado como Diplomado en Ciencias Sociales en la Academia de Humanismo Cristiano y Magíster en Desarrollo Urbano de la Universidad de Lovaina, Bélgica. A continuación presentamos la entrevista concedida por Juan Pablo Gramsch a nuestra revista.

  7. Evolution of the western segment of Juan Fernández Ridge (Nazca Plate): plume vs. plate tectonic processes

    Science.gov (United States)

    Lara, Luis E.; Rodrigo, Cristián; Reyes, Javier; Orozco, Gabriel

    2014-05-01

    The Juan Fernandez Ridge (Eastern Pacific, Nazca Plate) is thought to be a classic hot spot trail because of the apparent age progression observed in 40Ar-39Ar data. However, geological evidence and some thermochronological data suggest a more complex pattern with a rejuvenation stage in Robinson Crusoe Island, the most eroded of the Juan Fernandez Archipelago. In fact, a postshield stage at 900-700 ka separates the underlying shield-related pile from the post-erosional alkaline succession (Ba/Yb=38.15; La/Yb=15.66; Ba/Y=20.27; Ba/Zr=2.31). Shield volcanoes grew at high effusion rate at ca. 5-4 Ma erupting mostly tholeiitic to transitional magmas (Ba/Yb=18.07-8.32; La/Yb=4.59-9.84; Ba/Y=4.24-8.18; Ba/Zr=0.73-1.09). Taken together, shield volcanoes form a continuous plateau with a base at ca. 3900 mbsl. However, a more complex structural pattern can be inferred from geophysical data, which suggest some intracrustal magma storage and a more extended area of magma ascent. A role for the Challenger Fracture Zone is hypothesized fueling the controversy between pristine plume origin and the effect of plate tectonic processes in the origin of intraplate volcanism. This research is supported by FONDECYT Project 1110966.

  8. India-Eurasia collision triggers formation of an oceanic microplate

    Science.gov (United States)

    Matthews, Kara; Müller, Dietmar; Sandwell, David

    2016-04-01

    Detailed mapping of seafloor tectonic fabric in the Indian Ocean, using high-resolution satellite-derived vertical gravity gradient data, reveals an extinct Pacific-style oceanic microplate - the Mammerickx Microplate - west of the Ninetyeast Ridge. It is one of the first Pacific-style microplates to be mapped outside the Pacific basin, suggesting that geophysical conditions during formation probably resembled those that have dominated at eastern Pacific ridges. The microplate formed at the Indian-Antarctic ridge and is bordered by an extinct ridge in the north and pseudofault in the south, whose conjugate is located north of the Kerguelen Plateau. Independent microplate rotation is indicated by asymmetric pseudofaults and rotated abyssal hill fabric, also identified in multibeam data. Magnetic anomaly picks and age estimates calculated from published spreading rates suggest formation during chron 21o (~47.3 Ma). Plate reorganizations can trigger ridge propagation and microplate development, and we propose that formation of the Mammerickx Microplate is linked with the initial 'soft' stage of the India-Eurasia collision. The collision altered the stress regime at the Indian-Antarctic ridge, leading to a change in segmentation and ridge propagation from an establishing transform fault. Fast Indian-Antarctic spreading that preceded microplate formation, and Kerguelen Plume activity may have facilitated ridge propagation via the production of thin and weak lithosphere. However, both factors had been present for tens of millions of years and are therefore unlikely to have triggered the event. Prior to the collision, this combination of fast spreading and plume activity was responsible for the production of a wide region of undulate seafloor to the north of the extinct ridge and 'W' shaped lineations that record back and forth ridge propagation. Microplate formation provides a means of dating the onset of the India-Eurasia collision, and is completely independent of and

  9. Tectonic microplates in a wax model of sea-floor spreading

    Science.gov (United States)

    Katz, Richard F.; Ragnarsson, Rolf; Bodenschatz, Eberhard

    2005-01-01

    Rotating, growing microplates are observed in a wax analogue model of sea-floor spreading. Wax microplates are kinematically similar to sea-floor tectonic microplates in terms of spreading rate and growth rate. Furthermore, their spiral pseudofault geometry is quantitatively consistent with Schouten's oceanic microplate model. These results suggest that Schouten's edge-driven microplate model captures the kinematics of tectonic microplate evolution on Earth. Based on the wax observations, a theory for the nucleation of overlapping spreading centres, the precursors of tectonic microplates, is developed.

  10. Initially Imperfect MEMS Microplates Under Electrostatic Actuation: Theory and Experiment

    KAUST Repository

    Saghir, Shahid

    2016-12-05

    Microplates are building blocks of many Micro-Electro-Mechanical Systems (MEMS). It is common for them to undergo imperfections due to residual stresses caused by the micro fabrication process. Such plates are essentially different from perfectly flat plates and cannot be modeled using the governing equations of flat plates. In this article, we adopt the governing equations of imperfect plates employing the modified von-Karman strains. These equations then are used to develop a Reduced Order Model based on the Galerkin procedure to simulate the static and dynamic behavior of an electrostatically actuated microplate. Also, microplates made of silicon nitride are fabricated and tested. First, the static behaviour of the microplate is investigated when applying a static voltage Vdc. To study the dynamic behaviour we apply a harmonic voltage, Vac, superimposed to Vdc. Simulation results show good agreement with the experimentally measured responses.

  11. Remembering Juan Navia.

    Science.gov (United States)

    Dasanayake, A P; Li, Y; Maetz, H M; Vermund, S H

    2013-10-01

    Juan Navia died on September 4, 2010. Those who knew him as the director of the University of Alabama's John J. Sparkman Center for International Public Health Education and later the dean of UAB School of Public Health watched him train and shape the next generation of global public health leaders with a kind heart and a firm, but gentle, hand. On this third anniversary of Professor Navia's passing, in response to an invitation from the Journal of Dental Research to write an essay on an educator who influenced the professional trajectories of many people, we have put together an account of some of his contributions and attributes to highlight this remarkable leader's accomplishments in and impact on dental public health and global nutrition.

  12. Conversation with Juan Carlos Negrete.

    Science.gov (United States)

    Negrete, Juan Carlos

    2013-08-01

    Juan Carlos Negrete is Emeritus Professor of Psychiatry, McGill University; Founding Director, Addictions Unit, Montreal General Hospital; former President, Canadian Society of Addiction Medicine; and former WHO/PAHO Consultant on Alcoholism, Drug Addiction and Mental Health.

  13. Nonlinear Mechanics of MEMS Rectangular Microplates under Electrostatic Actuation

    KAUST Repository

    Saghir, Shahid

    2016-12-01

    The first objective of the dissertation is to develop a suitable reduced order model capable of investigating the nonlinear mechanical behavior of von-Karman plates under electrostatic actuation. The second objective is to investigate the nonlinear static and dynamic behavior of rectangular microplates under small and large actuating forces. In the first part, we present and compare various approaches to develop reduced order models for the nonlinear von-Karman rectangular microplates actuated by nonlinear electrostatic forces. The reduced-order models aim to investigate the static and dynamic behavior of the plate under small and large actuation forces. A fully clamped microplate is considered. Different types of basis functions are used in conjunction with the Galerkin method to discretize the governing equations. First we investigate the convergence with the number of modes retained in the model. Then for validation purpose, a comparison of the static results is made with the results calculated by a nonlinear finite element model. The linear eigenvalue problem for the plate under the electrostatic force is solved for a wide range of voltages up to pull-in. In the second part, we present an investigation of the static and dynamic behavior of a fully clamped microplate. We investigate the effect of different non-dimensional design parameters on the static response. The forced-vibration response of the plate is then investigated when the plate is excited by a harmonic AC load superimposed to a DC load. The dynamic behavior is examined near the primary and secondary (superharmonic and subharmonic) resonances. The microplate shows a strong hardening behavior due to the cubic nonlinearity of midplane stretching. However, the behavior switches to softening as the DC load is increased. Next, near-square plates are studied to understand the effect of geometric imperfections of microplates. In the final part of the dissertation, we investigate the mechanical behavior of

  14. Initially curved microplates under electrostatic actuation: theory and experiment

    KAUST Repository

    Saghir, S.

    2016-07-01

    Microplates are the building blocks of many micro-electro-mechanical systems. It is common for them to experience initial curvature imperfection due to residual stresses caused by the micro fabrication process. Such plates are essentially different from perfectly flat ones and cannot be modeled using flat plate models. In this paper, we adopt a dynamic analog of the von Karman governing equations of imperfect plates. These equations are then used to develop a reduced order model based on the Galerkin procedure, to simulate the static and dynamic behavior of the microplate under electrostatic actuation. To validate the simulation results, an initially curved imperfect microplate made of silicon nitride is fabricated and tested. The static behaviour of the microplate is investigated when applying a DC voltage Vdc. Then, the dynamic behaviour of the microplate is examined under the application of a harmonic AC voltage, Vac, superimposed to Vdc. The simulation results show good agreement with the experimentally measured responses. © 2016 IOP Publishing Ltd.

  15. Entrevista con Juan Marichal.

    Directory of Open Access Journals (Sweden)

    - Consejo de Redacción

    1997-01-01

    Full Text Available Juan Marichal nació en Santa Cruz de Tenerife, en 1922, en el seno de una familia ligada al partido republicano insular. Muy joven, en 1935, se trasladó a Madrid, ciudad en la que vive el estallido de la guerra civil. En 1937, pasa a Valencia y luego a Barcelona; tras su exilio en 1938, prosigue sus estudios secundarios en un liceo de París, concluyéndolos en Casablanca. En 1941 emigra a México, formándose en la UNAM: fue alumno de los exiliados José Gaos y Joaquín Xirau así como del mexicano Edmundo O 'Gorman. Luego, becado en Princeton desde 1946, lo fue de América Castro, donde preparó una tesis sobre Feijoo. Apoyado en las vastas perspectivas de sus maestros, fue orientándose hada nuestra historia intelectual, desde el siglo XV hasta hoy. Su carrera profesional se ha desarrollado en los Estados Unidos (coincidiendo con Amado Alonso y con Ferrater Mora: ha sido profesor de estudios hispánicos en la Universidad de Harvard, desde 1948 hasta 1988, año en que se jubiló voluntariamente como numerario (aunque había permanecido en el Bryn Mawr College, entre 1953 y 1957. A este trabajo se suman, con todo, sus conferencias en América Latina y en España. Ha colaborado en las revistas más importantes, en este campo, de México, Nueva York, Puerto Rico, La Habana, Buenos Aires o París así como de las españolas, desde los sesenta. Juan Marichal -hoy, miembro de la Junta Directiva de los Amigos de la Residencia de Estudiantes, director del Boletín de la Institución Libre de Enseñanza y asociado al Instituto Universitario Ortega y Gasset-, reside en España desde otoño de 1989: se considera a sí mismo «voluntario en Madrid», como había dicho Alfonso Reyes en su estancia madrileña (1914-1924.

  16. Microplate-based method for high-throughput screening of microalgae growth potential

    DEFF Research Database (Denmark)

    Van Wagenen, Jonathan; Holdt, Susan Løvstad; De Francisci, Davide

    2014-01-01

    Microalgae cultivation conditions in microplates will differ from large-scale photobioreactors in crucial parameters such as light profile, mixing and gas transfer. Hence volumetric productivity (Pv) measurements made in microplates cannot be directly scaled up. Here we demonstrate that it is pos......Microalgae cultivation conditions in microplates will differ from large-scale photobioreactors in crucial parameters such as light profile, mixing and gas transfer. Hence volumetric productivity (Pv) measurements made in microplates cannot be directly scaled up. Here we demonstrate...

  17. Microplate Bioassay for Determining Substrate Selectivity of "Candida rugosa" Lipase

    Science.gov (United States)

    Wang, Shi-zhen; Fang, Bai-shan

    2012-01-01

    Substrate selectivity of "Candida rugosa" lipase was tested using "p"-nitrophenyl esters of increasing chain length (C[subscript 1], C[subscript 7], C[subscript 15]) using the high-throughput screening method. A fast and easy 96-well microplate bioassay was developed to help students learn and practice biotechnological specificity screen. The…

  18. Microplate Bioassay for Determining Substrate Selectivity of "Candida rugosa" Lipase

    Science.gov (United States)

    Wang, Shi-zhen; Fang, Bai-shan

    2012-01-01

    Substrate selectivity of "Candida rugosa" lipase was tested using "p"-nitrophenyl esters of increasing chain length (C[subscript 1], C[subscript 7], C[subscript 15]) using the high-throughput screening method. A fast and easy 96-well microplate bioassay was developed to help students learn and practice biotechnological specificity screen. The…

  19. Antibiotic microbial assay using kinetic-reading microplate system

    OpenAIRE

    Felipe Rebello Lourenço; Terezinha de Jesus Andreoli Pinto

    2011-01-01

    The aim of this study was to determine the optimal experimental conditions to develop a methodology for microbiological assay of apramycin employing microplate and kinetic reading mode, and to validate the developed method, through evaluation of parameters of selectivity, linearity, linear range, limits of detection and quantification, accuracy and precision. The turbidimetric assay principle is simple: the test solution is added to a suspension of test microorganism in culture media, the mix...

  20. GPS estimates of microplate motions, northern Caribbean: evidence for a Hispaniola microplate and implications for earthquake hazard

    Science.gov (United States)

    Benford, B.; DeMets, C.; Calais, E.

    2012-09-01

    We use elastic block modelling of 126 GPS site velocities from Jamaica, Hispaniola, Puerto Rico and other islands in the northern Caribbean to test for the existence of a Hispaniola microplate and estimate angular velocities for the Gônave, Hispaniola, Puerto Rico-Virgin Islands and two smaller microplates relative to each other and the Caribbean and North America plates. A model in which the Gônave microplate spans the whole plate boundary between the Cayman spreading centre and Mona Passage west of Puerto Rico is rejected at a high confidence level. The data instead require an independently moving Hispaniola microplate between the Mona Passage and a likely diffuse boundary within or offshore from western Hispaniola. Our updated angular velocities predict 6.8 ± 1.0 mm yr-1 of left-lateral slip along the seismically hazardous Enriquillo-Plantain Garden fault zone of southwest Hispaniola, 9.8 ± 2.0 mm yr-1 of slip along the Septentrional fault of northern Hispaniola and ˜14-15 mm yr-1 of left-lateral slip along the Oriente fault south of Cuba. They also predict 5.7 ± 1 mm yr-1 of fault-normal motion in the vicinity of the Enriquillo-Plantain Garden fault zone, faster than previously estimated and possibly accommodated by folds and faults in the Enriquillo-Plantain Garden fault zone borderlands. Our new and a previous estimate of Gônave-Caribbean plate motion suggest that enough elastic strain accumulates to generate one to two Mw˜ 7 earthquakes per century along the Enriquillo-Plantain Garden and nearby faults of southwest Hispaniola. That the 2010 M= 7.0 Haiti earthquake ended a 240-yr-long period of seismic quiescence in this region raises concerns that it could mark the onset of a new earthquake sequence that will relieve elastic strain that has accumulated since the late 18th century.

  1. Enhancement of Fluorescence-Based Sandwich Immunoassay Using Multilayered Microplates Modified with Plasma-Polymerized Films

    Directory of Open Access Journals (Sweden)

    Kazuyoshi Yano

    2016-12-01

    Full Text Available A functional modification of the surface of a 96-well microplate coupled with a thin layer deposition technique is demonstrated for enhanced fluorescence-based sandwich immunoassays. The plasma polymerization technique enabling the deposition of organic thin films was employed for the modification of the well surface of a microplate. A silver layer and a plasma-polymerized film were consecutively deposited on the microplate as a metal mirror and the optical interference layer, respectively. When Cy3-labeled antibody was applied to the wells of the resulting multilayered microplate without any immobilization step, greatly enhanced fluorescence was observed compared with that obtained with the unmodified one. The same effect could be also exhibited for an immunoassay targeting antigen directly adsorbed on the multilayered microplate. Furthermore, a sandwich immunoassay for the detection of interleukin 2 (IL-2 was performed with the multilayered microplates, resulting in specific and 88-fold–enhanced fluorescence detection.

  2. Juan Villoro: miradas del ensayo

    OpenAIRE

    Walker, Carlos

    2009-01-01

    El presente trabajo analiza los dos libros que el escritor mexicano Juan Villoro inscribe bajo la rúbrica del ensayo -Efectos personales (2001) y De eso se trata (2007). Dicho análisis dará pie a un desarrollo sobre el lugar del ensayo como terreno privilegiado para la reflexión crítica. A partir de los célebres textos que György Lukács y Theodor Adorno dedicaran a la forma del ensayo, se establece una divergencia en los métodos que uno y otro proponen para el quehacer del ensayista. Lukács a...

  3. a Model of the Kinematics of the Danakil Microplate

    Science.gov (United States)

    Gloaguen, R.; Eagles, G.

    2001-12-01

    We present a regional approach to the tectonics of the Afar depression, in which we attempt to relate reconnaissance-scale data to overall movement of a Danakil microplate. Topographic, satellite, airphoto, magnetic and gravity data from the flanks and interior of the Afar depression, NE Africa, are used to assess differing models of the dispersal of the Danakil microplate from its western to its eastern margin. The data are compared to small circles about published instantaneous poles to find evidence of strike-slip and transverse features. A good fit to such features is made using a statistically-significant modern pole to the NNE of the Afar depression. A possible reconstruction about this pole, which reunites branches of an inherited shear zone, is presented. This reconstruction is not consistent with a `crank-arm' or `saloon-doors' mode of opening for the Afar depression. Instead we suggest that only recently has the Danakil microplate come into existence, after the westwards propagation of the Gulf of Aden rift into the Afar depression. Prior to this time movement of Danakil occurred very slowly about the same pole, but as part of the Somalian plate; as shown by the continuity of ancient flowline features onto the Nubian margin of the Main Ethiopian Rift. At 7 Ma, possibly due to the onset of oceanic-type accretion in Afar where the lithosphere was hot and thin already from the presence of a hotspot, Danakil accelerated eastwards about this pole. In response the Gulf of Aden rift propagated westwards to form a new plate boundary between Danakil and Somalia. The newly-formed Danakil microplate is thus an intermediate stage in the transfer of all or part of the Danakil horst to the Arabian plate from the Somalian plate. Further south, at the Main Ethiopian Rift, continental lithosphere did not rupture but the presence of focussed accretion at Afar in a hitherto-diffuse plate boundary precipitated the southward migration of the Somalia-Nubia pole to its present

  4. Ejection of small droplet from microplate using focused ultrasound

    Science.gov (United States)

    Tanaka, Hiroki; Mizuno, Yosuke; Nakamura, Kentaro

    2017-08-01

    We discussed an ultrasonic system for single-droplet ejection from a microplate, which is one of the basic and important procedures in the noncontact handling of droplets in air. In this system, a 1.5 MHz concave transducer located below the microplate is used for chasing the liquid surface through a pulse echo method, and also for the ejection of a 1 µL single droplet by the burst of focused ultrasound. We investigated the relationship between the droplet ejection characteristics, the distance from the transducer to the surface of liquid, the material property, and the excitation condition of the focused ultrasonic transducer. It was verified that the optimal position of the transducer was off the focal point of sound pressure by ±1 mm, because the sound intensity had to be controlled to eject a single droplet. Subsequently, we confirmed experimentally that the ejected droplet volume linearly depended on the surface tension of the liquid, and that the droplet volume and ejection velocity were determined by the Webber number, Reynolds number, and Ohnesolge number. In addition, by optimizing the duration of the burst ultrasound, the droplet volume and ejection velocity were controlled.

  5. Trois biographies de Juan Rulfo

    OpenAIRE

    2014-01-01

    L’article s’intéresse à la fortune éditoriale des biographies de Rulfo à l’occasion de la célébration des cinquante ans de la parution de El llano en llamas (1953) et de Pedro Páramo (1955). L’étude de trois d’entre elles (écrites par Nuria Amat, Reina Roffé et Juan Ascensio) permet de voir le processus de légitimation de la biographie et les positions qu’adoptent les différents auteurs (tout spécialement en ce qui concerne leur relation avec l’auteur “biographié”) Les auteurs justifient leur...

  6. Juan de Mairena en Srebrenica

    Directory of Open Access Journals (Sweden)

    Sánchez, J. Jorge

    1997-09-01

    Full Text Available Not available.Dos hilos impulsan estas líneas: la reflexión sobre uno de los acontecimientos cruciales de este fin de siglo en Europa, la masacre de Srebrenica, y la necesidad de contribuir a la tarea colectiva de reconstruir una tradición filosófica en lengua castellana más allá de las clásicas distinciones de géneros. Ambos propósitos confluirían, metonímica y metafóricamente, en un nombre, Machado, y una obra, el Juan de Mairena. Los textos del apócrifo del poeta, tan próximo en algunos momentos al pensamiento de Lévinas o Heidegger, pueden contribuir a articular un examen crítico, insertado en una tradición, sobre ese símbolo de nuestra época.

  7. Graafikatriennaalil osalev Juan Manuel Echavarria saabub Tallinna

    Index Scriptorium Estoniae

    2011-01-01

    12.-15. aprillini 2011 külastab Tallinna Columbia kunstnik Juan Manuel Echavarria. Tema holograafiliste trükiste sarjast "Reekviem NN" (2008-2010), mida eksponeeritakse Tallinna XV graafikatriennaalil Kumu Kunstimuuseumis

  8. Graafikatriennaalil osalev Juan Manuel Echavarria saabub Tallinna

    Index Scriptorium Estoniae

    2011-01-01

    12.-15. aprillini 2011 külastab Tallinna Columbia kunstnik Juan Manuel Echavarria. Tema holograafiliste trükiste sarjast "Reekviem NN" (2008-2010), mida eksponeeritakse Tallinna XV graafikatriennaalil Kumu Kunstimuuseumis

  9. Photosensitizer Adhered to Cell Culture Microplates Induces Phototoxicity in Carcinoma Cells

    Directory of Open Access Journals (Sweden)

    Verena Ziegler

    2013-01-01

    Full Text Available In vitro experiments in plastic receptacles are the basis of characterization of new photosensitizers (PSs for the photodynamic therapy. We recently reported that lipophilic PSs adhere to cell culture microplates in a kinetic-like manner (Engelhardt et al., 2011. In the current study, we examined the interaction and phototoxic effects of the microplate-adhered PS in cancer cells. Therefore, we preloaded microplates with hypericin, Foscan, PVP-hypericin, or aluminum (III phthalocyanine tetrasulfonate chloride (AlPCS4 for 24 hours and measured the PS distribution after addition of A431 human carcinoma cells: following another 24 hours up to 68% of hypericin were detected in the cell fraction. The hydrophilic PVP-hypericin and AlPCS4 also diffused into the cells, but the quantities of PS adherence were considerably lower. Microplate-adhered Foscan appeared not to be redistributed. In contrast to the hydrophilic PSs, the cellular phototoxicity of microplate-adhered lipophilic PS was high, independent of whether the PS (i was pre-loaded onto microplates or (ii added simultaneously with the cells or (iii one day after cell seeding. Based on these results, we suggest testing lipophilic PS dyes for their adherence to microplates. Furthermore, the ability of plastic materials to (reversibly store PSs might represent a new approach for the PS delivery or the development of antimicrobial coatings.

  10. Oceanic microplate formation records the onset of India-Eurasia collision

    Science.gov (United States)

    Matthews, Kara J.; Dietmar Müller, R.; Sandwell, David T.

    2016-01-01

    Mapping of seafloor tectonic fabric in the Indian Ocean, using high-resolution satellite-derived vertical gravity gradient data, reveals an extinct Pacific-style oceanic microplate ('Mammerickx Microplate') west of the Ninetyeast Ridge. It is one of the first Pacific-style microplates to be mapped outside the Pacific basin, suggesting that geophysical conditions during formation probably resembled those that have dominated at eastern Pacific ridges. The microplate formed at the Indian-Antarctic ridge and is bordered by an extinct ridge in the north and pseudofault in the south, whose conjugate is located north of the Kerguelen Plateau. Independent microplate rotation is indicated by asymmetric pseudofaults and rotated abyssal hill fabric, also seen in multibeam data. Magnetic anomaly picks and age estimates calculated from published spreading rates suggest formation during chron 21o (∼47.3 Ma). Plate reorganizations can trigger ridge propagation and microplate development, and we propose that Mammerickx Microplate formation is linked with the India-Eurasia collision (initial 'soft' collision). The collision altered the stress regime at the Indian-Antarctic ridge, leading to a change in segmentation and ridge propagation from an establishing transform. Fast Indian-Antarctic spreading that preceded microplate formation, and Kerguelen Plume activity, may have facilitated ridge propagation via the production of thin and weak lithosphere; however both factors had been present for tens of millions of years and are therefore unlikely to have triggered the event. Prior to the collision, the combination of fast spreading and plume activity was responsible for the production of a wide region of undulate seafloor to the north of the extinct ridge and 'W' shaped lineations that record back and forth ridge propagation. Microplate formation provides a precise means of dating the onset of the India-Eurasia collision, and is completely independent of and complementary to timing

  11. 33 CFR 165.754 - Safety Zone: San Juan Harbor, San Juan, PR.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Safety Zone: San Juan Harbor, San Juan, PR. 165.754 Section 165.754 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY REGULATED NAVIGATION AREAS AND LIMITED ACCESS AREAS Specific Regulated Navigation Areas and...

  12. Fray Juan Rizi en Italia

    Directory of Open Access Journals (Sweden)

    Salort Pons, Salvador

    1999-03-01

    Full Text Available Since Tormo, Gusi and Lafuente wrote fray Juan Rizi's monograph in 1930, and Ángulo & Pérez Sánchez revised years later this study in their book Pintura madrileña del segundo tercio del siglo XVII, very little has been published about the life and work of the Benedictine painter. Furthermore, the Italian journey of Rizi, that involved the last 20 years of his life, has always been an enigma, resolved only in certain aspects, thanks to few news supplied by his biographers and the manuscripts the monk wrote during his staying in the Montecassino abbey. Our recent discovery of 8 new canvases painted by Rizi to decorate the Saints Cosme and Damian's chapel in the main church of Trevi nel Lazio (Frosinone, Italy, as well as the location of several new drawings in the Montecassino and Vatican libraries, will allow us to study his activity as painter in Italy and also to profile, with more accuracy, the history of the last years of his life.No disponible

  13. Validation of spectrophotometric microplate methods for polyphenol oxidase and peroxidase activities analysis in fruits and vegetables

    Directory of Open Access Journals (Sweden)

    Érica Sayuri SIGUEMOTO

    Full Text Available Abstract Enzymes polyphenol oxidase (PPO and peroxidase (POD play important roles in the processing of fruits and vegetables, since they can produce undesirable changes in color, texture and flavor. Classical methods of activity assessment are based on cuvette spectrophotometric readings. This work aims to propose, to validate and to test microplate spectrophotometric methods. Samples of apple juice and lyophilized enzymes from mushroom and horseradish were analyzed by the cuvette and microplate methods and it was possible to validate the microplate assays with satisfactory results regarding linearity, repeatability, accuracy along with quantitation and detection limits. The proposed microplate methods proved to be reliable and reproducible as the classical methods besides having the advantages of allowing simultaneous analysis and requiring a reduced amount of samples and reactants, which can beneficial to the study of enzyme inactivation in the processing of fruits and vegetables.

  14. Media selection in ion-exchange chromatography in a single microplate.

    Science.gov (United States)

    Cabanne, Charlotte; Santarelli, Xavier

    2014-01-01

    High-throughput process development is more and more used in chromatography. Limitations are the tools provided by the manufacturers. Here, we describe a method to select chromatographic media for ion-exchange chromatography using a 96-well filter microplate.

  15. 76 FR 12692 - San Juan National Forest Resource Advisory Committee

    Science.gov (United States)

    2011-03-08

    ... Forest Service San Juan National Forest Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The San Juan National Forest Resource Advisory Council (RAC) will meet in... comments should be sent to Attn: San Juan National Forest RAC, 15 Burnett Court, Durango, CO 81301...

  16. 76 FR 40876 - San Juan National Forest Resource Advisory Committee

    Science.gov (United States)

    2011-07-12

    ... Forest Service San Juan National Forest Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The San Juan National Forest Resource Advisory Council (RAC) will meet in... Sonoran Meeting Rooms. Written comments should be sent to Attn: San Juan National Forest RAC, 15 Burnett...

  17. 75 FR 48306 - San Juan National Forest Resource Advisory Committee

    Science.gov (United States)

    2010-08-10

    ... Forest Service San Juan National Forest Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The San Juan National Forest Resource Advisory Council (RAC) will meet in... comments should be sent to Attn: San Juan National Forest RAC, 15 Burnett Court, Durango, CO 81301...

  18. Juan Eduardo Cirlot on Skryabin's Synesthetic Art

    OpenAIRE

    2012-01-01

    En este trabajo mostramos cómo el poeta y pensador español Juan Eduardo Cirlot (Barcelona, 1916–1973) y el compositor ruso Aleksandr Skriabin compartieron un interés artístico poro el fenómeno de la sinestesia, a pesar de la distancia cronológica entre ambos y la falta de relaciones diplomáticas y (al menos, oficialmente) culturales entre sus respectivos países, durante la vida creativa de Juan Eduardo Cirlot.

  19. San Juan Islands National Wildlife Refuge : San Juan Wilderness : Wilderness management plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This document is a plan regarding management of the San Juan Wilderness. After introducing the area, it analyzes current management practices against current public...

  20. Saga y fugas de Don Juan

    OpenAIRE

    Pérez-Bustamante Mourier, Ana Sofía

    1998-01-01

    Ensayo acerca de las diversas versiones e interpretaciones que a lo largo de la historia ha suscitado el mito de Don Juan Tenorio. Lectura original del donjuanismo a partir de las teorías del filósofo José Antonio Marina en torno al ingenio y sus productos.

  1. On Byronic Heroism in Don Juan

    Institute of Scientific and Technical Information of China (English)

    李曼琦

    2011-01-01

    Byron is the chief exponent and the most important figure of the "Romantic Movement".And Byronic heroism is one of his most important representatives of his literary contribution.In his masterpiece Don Juan,Byron exploits the whole range of his feelings a

  2. Juan Carlos D'Olivo: A portrait

    Science.gov (United States)

    Aguilar-Arévalo, Alexis A.

    2013-06-01

    This report attempts to give a brief bibliographical sketch of the academic life of Juan Carlos D'Olivo, researcher and teacher at the Instituto de Ciencias Nucleares of UNAM, devoted to advancing the fields of High Energy Physics and Astroparticle Physics in Mexico and Latin America.

  3. Antibiotic microbial assay using kinetic-reading microplate system

    Directory of Open Access Journals (Sweden)

    Felipe Rebello Lourenço

    2011-09-01

    Full Text Available The aim of this study was to determine the optimal experimental conditions to develop a methodology for microbiological assay of apramycin employing microplate and kinetic reading mode, and to validate the developed method, through evaluation of parameters of selectivity, linearity, linear range, limits of detection and quantification, accuracy and precision. The turbidimetric assay principle is simple: the test solution is added to a suspension of test microorganism in culture media, the mixture is incubated under appropriate conditions and the microbial growth is measured by photometric reading. Microplate with kinetic reading mode employed in antibiotic assay is of considerable interest since it allows reduction of material and analysis time and enables a large number of samples to be analyzed simultaneously, with automated reading and calculating. Established conditions considered the standard-curve of apramycin at concentrations from 5.0 to 35.0 μg mL-1, and tryptic soy broth inoculated with 5% Escherichia coli (ATCC 8739 suspension. Satisfactory results were obtained with 2 hours of incubation. The developed method showed appropriate selectivity, linearity in the range from 5.0 to 35.0 μg mL-1, limits of detection and quantification of 0.1 and 0.4 μg mL-1, respectively, as well as satisfactory accuracy (recuperation = 98.5% and precision (RSD = 6.0%. Microplate assay combined the characteristics of microbiological (evaluation of antibiotic activity against sensitive test microorganism and physico-chemical (operationally straightforward and faster results assays.O objetivo deste trabalho é determinar as condições experimentais ideais para o desenvolvimento de metodologia para a dosagem microbiológica de apramicina empregando microplacas e modo de leitura cinético e validar o método desenvolvido, através da avaliação dos parâmetros de especificidade e seletividade, linearidade, faixa ou intervalo linear, limite de detecção e

  4. Novel versatile smart phone based Microplate readers for on-site diagnoses.

    Science.gov (United States)

    Fu, Qiangqiang; Wu, Ze; Li, Xiuqing; Yao, Cuize; Yu, Shiting; Xiao, Wei; Tang, Yong

    2016-07-15

    Microplate readers are important diagnostic instruments, used intensively for various readout test kits (biochemical analysis kits and ELISA kits). However, due to their expensive and non-portability, commercial microplate readers are unavailable for home testing, community and rural hospitals, especially in developing countries. In this study, to provide a field-portable, cost-effective and versatile diagnostic tool, we reported a novel smart phone based microplate reader. The basic principle of this devise relies on a smart phone's optical sensor that measures transmitted light intensities of liquid samples. To prove the validity of these devises, developed smart phone based microplate readers were applied to readout results of various analytical targets. These targets included analanine aminotransferase (ALT; limit of detection (LOD) was 17.54 U/L), alkaline phosphatase (AKP; LOD was 15.56 U/L), creatinine (LOD was 1.35μM), bovine serum albumin (BSA; LOD was 0.0041mg/mL), prostate specific antigen (PSA; LOD was 0.76pg/mL), and ractopamine (Rac; LOD was 0.31ng/mL). The developed smart phone based microplate readers are versatile, portable, and inexpensive; they are unique because of their ability to perform under circumstances where resources and expertize are limited. Copyright © 2016 Elsevier B.V. All rights reserved.

  5. Work function engineering of SnO single crystal microplates with thermal annealing.

    Science.gov (United States)

    Doh, Won Hui; Jeong, Wooseok; Lee, Hyunsoo; Park, Jonghyurk; Park, Jeong Young

    2016-08-19

    We synthesized black SnO single-crystal microplates via a sonochemical process and engineered the work function of the SnO microplates using thermal treatments. The as-synthesized SnO microplates have a wide (001) plane, as is clearly evident from TEM images and diffraction patterns. Surface potential measurements on the SnO microplates show that the work function changes as the annealing temperature increases. The TEM and XAS results after thermal treatments imply that the micro-sized SnO(001) single-crystals are stable up to about 400 °C in air, after which the surface starts to become locally oxidized. Consequently, the long-range ordering and lattice parameter of the SnO(001) single crystals started to change to make polycrystalline SnO2 at about 600 °C. These results demonstrate the ability to tune the work function of the microplates and suggest an intriguing way to engineer the electrical properties of nanostructures.

  6. Approaches for Reduced Order Modeling of Electrically Actuated von Karman Microplates

    KAUST Repository

    Saghir, Shahid

    2016-07-25

    This article presents and compares different approaches to develop reduced order models for the nonlinear von Karman rectangular microplates actuated by nonlinear electrostatic forces. The reduced-order models aim to investigate the static and dynamic behavior of the plate under small and large actuation forces. A fully clamped microplate is considered. Different types of basis functions are used in conjunction with the Galerkin method to discretize the governing equations. First we investigate the convergence with the number of modes retained in the model. Then for validation purpose, a comparison of the static results is made with the results calculated by a nonlinear finite element model. The linear eigenvalue problem for the plate under the electrostatic force is solved for a wide range of voltages up to pull-in. Results among the various reduced-order modes are compared and are also validated by comparing to results of the finite-element model. Further, the reduced order models are employed to capture the forced dynamic response of the microplate under small and large vibration amplitudes. Comparison of the different approaches are made for this case. Keywords: electrically actuated microplates, static analysis, dynamics of microplates, diaphragm vibration, large amplitude vibrations, nonlinear dynamics

  7. San Juan Uchucuanicu: évolution historique

    Directory of Open Access Journals (Sweden)

    1975-01-01

    Full Text Available La communauté de San Juan est reconnue depuis 1939. Une première partie concerne l’organisation de la reducción de San Juan vers le milieu du XVIe siècle. Le poids fiscal s’exerce durement sur le village et la crise est générale dans toute la vallée du Chancay au XVIIe. siècle. La christianisation des habitants est définitive au milieu de ce même siècle. C’est vers la fin du XVIIe siècle et durant tout le XVIIIe que se multiplient les conflits entre San Juan et les villages voisins liés aux terrains de pâture et à la possession de l’eau. La deuxième partie du travail concerne les rapports de la communauté de San Juan avec le Pérou contemporain : contrainte fiscale toujours très lourde durant la fin de l’époque coloniale, exactions des militaires juste avant l’indépendance. La période républicaine voit toujours les conflits avec les villages voisins mais aussi la naissance de familles qui cherchent à retirer le maximum de la communauté. Les terres sont divisées et attribuées : la détérioration de l’organisation communale traditionnelle est manifeste. L4es conflits se multiplient entre petits propriétaires, mais aussi avec les haciendas voisines : c’est l’apparition d’une véritable lutte de classes. La situation actuelle est incertaine, le poids de l’économie marchande se développe avec l’exode des jeunes. Que sera la communauté San Juan à la fin de ce siècle? La comunidad de San Juan está reconocida desde 1939. La primera parte concierne a la organización de la 'reducción' de San Juan hacia mediados del siglo XVI. El peso fiscal se ejerce duramente sobre el pueblo y en el siglo XVII la crisis es general en todo el valle de Chancay. Hacia mediados del mismo siglo la cristianización de los habitantes es definitiva. Es hacia fines del siglo XVII y durante todo el siglo XVIII que se multiplican los conflictos entre San Juan y los pueblos vecinos, los que están relacionados con los terrenos de

  8. A direct heating model to overcome the edge effect in microplates.

    Science.gov (United States)

    Lau, Chun Yat; Zahidi, Alifa Afiah Ahmad; Liew, Oi Wah; Ng, Tuck Wah

    2015-01-01

    Array-based tests in a microplate format are complicated by the regional variation in results of the outer against the inner wells of the plate. Analysis of the evaporation mechanics of sessile drops showed that evaporation rate increase with temperature was due to changes in the heat of vaporization, density and diffusion coefficient. In simulations of direct bottom heating of standard microplates, considerable heat transfer via conduction from the side walls was found to be responsible for lower temperatures in the liquid in wells close to the edge. Applying a two temperature heating mode, 304 K at the side compared to 310 K at the bottom, allowed for a more uniform temperature distribution. Transparency microplates were found to inherently possess immunity to the edge effect problem due to the presence of air between the liquid and solid wall.

  9. Metabolic response induced by endophytic fungi and bacteria in H. marrubioides Epling in vitro microplants

    Directory of Open Access Journals (Sweden)

    Luciana Cristina Vitorino

    2013-01-01

    Full Text Available Hyptis marrubioides Epling is a native plant from Brazilian Cerrado. In this paper, the response of in vitro microplants of this species to inoculation with bacterial and fungal endophytic isolates is evaluated. HPLC-DAD analysis showed the presence of 3,4-O-(Z-dicaffeoylquinic acid and quercetin-7-O-glucoside as the main components. GC/MS analysis demonstrated that the sesquiterpenes τ-cadinol and caryophyllene oxide were only produced in microplants inoculated with endophytic bacteria, while methyl hexadecanoate, methyl heptadecanoate and methyl (Z,Z,Z 9,12,15-octadecatrienoate and the triterpene methyl 3β-hydroxy-urs-12-en-28-oate were overexpressed only when the microplant was treated with endophytic fungi.

  10. Direct monitoring of molecular recognition processes using fluorescence enhancement at colloid-coated microplates.

    Science.gov (United States)

    Lobmaier, C; Hawa, G; Götzinger, M; Wirth, M; Pittner, F; Gabor, F

    2001-01-01

    Direct monitoring of recognition processes at the molecular level is a valuable tool for studying reaction kinetics to assess affinity constants (e.g. drugs to receptors) and for designing rapid single step immunoassays. Methods currently used to gain information about binding processes predominantly depend on surface plasmon resonance. These systems use excitation with coherent light in attenuated total reflection geometry to obtain discrimination between surface-bound and free molecules in solution. Therefore labeling of the compounds is not necessary, but due to the complexity of the measuring setup the method is rather costly. In this contribution we present a simple method for performing kinetic single step biorecognition assays with fluorophore labeled compounds using the fluorescence enhancement properties of surface bound silver colloids. Silver colloids are bound to standard microplates via silanization of the plastic surface. Fluorophores close to this colloid coated surface show a significant gain in fluorescence compared to fluorophores farther away in the bulk solution. Therefore discrimination between surface bound and free fluorophores is possible and the binding of, for example, fluorophore labeled antibodies to antigens immobilized on the colloid surface results in increasing fluorescence intensity. Utilization of standard microplates makes this method fully compatible with conventional microplate processing and reading devices. Neither excitation with coherent laser light nor ATR geometry is required, the measurement is performed in a standard fluorescence microplate reader in front face geometry with a xenon flash lamp as excitation source. Methods for the preparation of colloid-coated microplates and fluorescence-enhanced biorecognition assays are presented. Additionally the dependence of the system performance on the structure and properties of the metal colloid coated surface is described. A two-component biorecognition model system shows a

  11. Application of a fluorometric microplate algal toxicity assay for riverine periphytic algal species.

    Science.gov (United States)

    Nagai, Takashi; Taya, Kiyoshi; Annoh, Hirochica; Ishihara, Satoru

    2013-08-01

    Although riverine periphytic algae attached to riverbed gravel are dominant species in flowing rivers, there is limited toxicity data on them because of the difficulty in cell culture and assays. Moreover, it is well known that sensitivity to pesticides differ markedly among species, and therefore the toxicity data for multiple species need to be efficiently obtained. In this study, we investigated the use of fluorometric microplate toxicity assay for testing periphytic algal species. We selected five candidate test algal species Desmodesmus subspicatus, Achnanthidium minutissimum, Navicula pelliculosa, Nitzschia palea, and Pseudanabaena galeata. The selected species are dominant in the river, include a wide range of taxon, and represent actual species composition. Other additional species were also used to compare the sensitivity and suitability of the microplate assay. A 96-well microplate was used as a test chamber and algal growth was measured by in-vivo fluorescence. Assay conditions using microplate and fluorometric measurement were established, and sensitivities of 3,5-dichlorophenol as a reference substance were assayed. The 50 percent effect concentrations (EC50s) obtained by fluorometric microplate assay and those obtained by conventional Erlenmeyer flask assay conducted in this study were consistent. Moreover, the EC50 values of 3,5-dichlorophenol were within the reported confidence intervals in literature. These results supported the validity of our microplate assay. Species sensitivity distribution (SSD) analysis was conducted using the EC50s of five species. The SSD was found to be similar to the SSD obtained using additional tested species, suggesting that SSD using the five species largely represents algal sensitivity. Our results provide a useful and efficient method for high-tier probabilistic ecological risk assessment of pesticides.

  12. Microplate-compatible total internal reflection fluorescence microscopy for receptor pharmacology

    Science.gov (United States)

    Chen, Minghan; Zaytseva, Natalya V.; Wu, Qi; Li, Min; Fang, Ye

    2013-05-01

    We report the use of total internal reflection fluorescence (TIRF) microscopy for analyzing receptor pharmacology and the development of a microplate-compatible TIRF imaging system. Using stably expressed green fluorescence protein tagged β2-adrenergic receptor as the reporter, we found that the activation of different receptors results in distinct kinetic signatures of the TIRF intensity of cells. These TIRF signatures closely resemble the characteristics of their respective label-free dynamic mass redistribution signals in the same cells. This suggests that TIRF in microplate can be used for profiling and screening drugs.

  13. Sobre Juan Bautista Maíno

    Directory of Open Access Journals (Sweden)

    Pérez Sánchez, Alfonso E.

    1997-06-01

    Full Text Available This article published new documents about the activity of the Domenican painter Juan Bautista Maíno specially concerning his concept of the practice of Painting in relation to the other arts. Works hitherto unknown are published here for the first time. Also illustrated are portraits of the Conde de Añover de Tajo and his wife, whose collections were, in addition given valuation by the artista.No disponible

  14. Sobre Juan Bautista Maíno

    OpenAIRE

    Pérez Sánchez, Alfonso E.

    1997-01-01

    This article published new documents about the activity of the Domenican painter Juan Bautista Maíno specially concerning his concept of the practice of Painting in relation to the other arts. Works hitherto unknown are published here for the first time. Also illustrated are portraits of the Conde de Añover de Tajo and his wife, whose collections were, in addition given valuation by the artista.No disponible

  15. Sobre el teatro del absurdo (de Juan del Encina a Juan Mateu

    Directory of Open Access Journals (Sweden)

    Serralta, Frédéric

    2005-06-01

    Full Text Available From the analysis of a few comic devices of the absurd in Don Juan Tenorio «El refugiao» —a parodical play by exiled playwright Juan Mateu, first performed in Toulouse in 1958—, this article aims at showing that a tradition is still alive —a tradition that can, non only be traced back to La venganza de don Mendo by Muñoz Seca (who did not, as has been claimed, invent the theatre of the absurd, but which is also part of an old trend stemming from Juan del Encina's famous Disparates, which has been exemplified by the burlesque plays of the seventeenth century, which was obvious in the nineteenth and early twentieth century popular liking for parodical drama, and which is still today one of the sources of what could be called humour the Spanish way.A partir del análisis de algunos mecanismos cómicos del absurdo en Don Juan Tenorio «El refugiao» —obra paródica del dramaturgo exiliado Juan Mateu estrenada en Toulouse en 1958—, el artículo pretende demostrar la persistencia de una tradición que no se remonta sólo a La venganza de don Mendo de Muñoz Seca (el cual no fue ni mucho menos, como demasiadas veces se ha dicho, el inventor del teatro del absurdo, sino que se inscribe en una vieja trayectoria arraigada en los famosos Disparates de Juan del Encina, ilustrada luego por la comedia burlesca del siglo XVII, patente en la afición popular del XIX y principios del XX al teatro paródico y que todavía hoy es una de las fuentes de lo que se podría llamar un humor «a la española».

  16. Gustatory response and appetitive learning in Microplitis croceipes in relation to sugar type and concentration

    NARCIS (Netherlands)

    Wäckers, F.L.; Bonifay, C.; Vet, L.E.M.; Lewis, W.J.

    2006-01-01

    Insects can be conditioned to respond to odours through associative learning. Various learning parameters, such as the rate of odour acquisition, are known to depend on the type of conditioned stimulus. Here we investigate to what extent appetitive conditioning in the parasitoid Microplitis croceipe

  17. Customizable PCR-microplate array for differential identification of multiple pathogens

    Science.gov (United States)

    Woubit, Abdela; Yehualaeshet, Teshome; Roberts, Sherrelle; Graham, Martha; Kim, Moonil; Samuel, Temesgen

    2014-01-01

    Customizable PCR-microplate arrays were developed for the rapid identification of Francisella tularensis subsp. tularensis, Salmonella Typhi, Shigella dysenteriae, Yersinia pestis, Vibrio cholerae Escherichia coli O157:H7, Salmonella Typhimurium, Salmonella Saintpaul, Francisella tularensis subsp. novicida, Vibrio parahaemolyticus, and Yersinia pseudotuberculosis. Previously, we identified highly specific primers targeting each of the pathogens above. Here, we report the development of customizable PCR-microplate arrays for simultaneous identification of the pathogens using the primers. A mixed aliquot of genomic DNA from 38 different strains was used to validate three PCR-microplate array formats. Identical PCR conditions were used to run all the samples on the three formats. Results show specific amplifications on all the three custom plates. In a preliminary test to evaluate the sensitivity of these assays in laboratory-inoculated samples, detection limits as low as 9 cfu/g/ml S. Typhimurium were obtained from beef hot dog, and 78 cfu/ml from milk. Such microplate arrays could serve as valuable tools for initial identification or secondary confirmation of these pathogens. PMID:24215700

  18. Assessment of a microplate method for detection of staphylococcal secretory inhibitor of platelet microbicidal protein.

    Science.gov (United States)

    Ivanov, Iuri B; Gritsenko, Viktor A

    2009-01-01

    We developed a novel microplate spectrophotometric assay for the detection of staphylococcal secretory inhibitor of platelet microbicidal protein (SIPMP) in

  19. Coupling the Torpedo microplate-receptor binding assay with mass spectrometry to detect cyclic imine neurotoxins.

    Science.gov (United States)

    Aráoz, Rómulo; Ramos, Suzanne; Pelissier, Franck; Guérineau, Vincent; Benoit, Evelyne; Vilariño, Natalia; Botana, Luis M; Zakarian, Armen; Molgó, Jordi

    2012-12-04

    Cyclic imine neurotoxins constitute an emergent family of neurotoxins of dinoflagellate origin that are potent antagonists of nicotinic acetylcholine receptors. We developed a target-directed functional method based on the mechanism of action of competitive agonists/antagonists of nicotinic acetylcholine receptors for the detection of marine cyclic imine neurotoxins. The key step for method development was the immobilization of Torpedo electrocyte membranes rich in nicotinic acetylcholine receptors on the surface of microplate wells and the use of biotinylated-α-bungarotoxin as tracer. Cyclic imine neurotoxins competitively inhibit biotinylated-α-bungarotoxin binding to Torpedo-nicotinic acetylcholine receptors in a concentration-dependent manner. The microplate-receptor binding assay allowed rapid detection of nanomolar concentrations of cyclic imine neurotoxins directly in shellfish samples. Although highly sensitive and specific for the detection of neurotoxins targeting nicotinic acetylcholine receptors as a class, the receptor binding assay cannot identify a given analyte. To address the low selectivity of the microplate-receptor binding assay, the cyclic imine neurotoxins tightly bound to the coated Torpedo nicotinic receptor were eluted with methanol, and the chemical nature of the eluted ligands was identified by mass spectrometry. The immobilization of Torpedo electrocyte membranes on the surface of microplate wells proved to be a high-throughput format for the survey of neurotoxins targeting nicotinic acetylcholine receptors directly in shellfish matrixes with high sensitivity and reproducibility.

  20. Una Visita al Viejo San Juan (A Visit to Old San Juan).

    Science.gov (United States)

    Cabello, Victor; And Others

    Written in Spanish, this black and white illustrated booklet provides a tour of Old San Juan, Puerto Rico's oldest and most historic city. Brief historical information is provided on the Perro de San Jeronimo, a statue of a barking dog found in front of the Castillo; Plaza de Colon, a small plaza dedicated to Christopher Columbus; the Catedral de…

  1. Una Visita al Viejo San Juan (A Visit to Old San Juan).

    Science.gov (United States)

    Cabello, Victor; And Others

    Written in Spanish, this black and white illustrated booklet provides a tour of Old San Juan, Puerto Rico's oldest and most historic city. Brief historical information is provided on the Perro de San Jeronimo, a statue of a barking dog found in front of the Castillo; Plaza de Colon, a small plaza dedicated to Christopher Columbus; the Catedral de…

  2. Chinanteco de San Juan Lealao, Oaxaca (Chinantec of San Juan Lealao, Oaxaca).

    Science.gov (United States)

    Mexico Coll. (Mexico City)

    This document is one of 17 volumes on indigenous Mexican languages and is the result of a project undertaken by the Archivo de Lenguas Indigenas de Mexico. This volume contains information on Chinantec, an indigenous language of Mexico spoken in San Juan Lealao, in the state of Oaxaca. The objective of collecting such a representative sampling of…

  3. Trique de San Juan Copala, Oaxaca (Trique of San Juan Copala, Oaxaca).

    Science.gov (United States)

    Mexico Coll. (Mexico City)

    This document is one of 17 volumes on indigenous Mexican languages and is the result of a project undertaken by the Archivo de Lenguas Indigenas de Mexico. This volume contains information on Trique, an indigenous language of Mexico spoken in San Juan Copala, in the state of Oaxaca. The objective of collecting such a representative sampling of the…

  4. Juan's Dilemma: A New Twist on the Old Lemon Battery

    Science.gov (United States)

    Hunt, Vanessa; Sorey, Timothy; Balandova, Evguenia; Palmquist, Bruce

    2010-01-01

    When life hands you lemons, make a battery! In this article, the authors describe an activity they refer to as "Juan's Dilemma," an extension of the familiar lemon-battery activity (Goodisman 2001). Juan's Dilemma integrates oxidation and reduction chemistry with circuit theory in a fun, real-world exercise. The authors designed this activity for…

  5. 33 CFR 80.1385 - Strait of Juan de Fuca.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Strait of Juan de Fuca. 80.1385 Section 80.1385 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Thirteenth District § 80.1385 Strait of Juan de Fuca. The 72 COLREGS shall apply on all waters of...

  6. 77 FR 47358 - San Juan National Forest Resource Advisory Committee

    Science.gov (United States)

    2012-08-08

    ... Forest Service San Juan National Forest Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The San Juan National Forest Resource Advisory Council (RAC) will meet in... recommendations to the Forest Service concerning projects and funding consistent with Title II of the Act. The...

  7. Juan's Dilemma: A New Twist on the Old Lemon Battery

    Science.gov (United States)

    Hunt, Vanessa; Sorey, Timothy; Balandova, Evguenia; Palmquist, Bruce

    2010-01-01

    When life hands you lemons, make a battery! In this article, the authors describe an activity they refer to as "Juan's Dilemma," an extension of the familiar lemon-battery activity (Goodisman 2001). Juan's Dilemma integrates oxidation and reduction chemistry with circuit theory in a fun, real-world exercise. The authors designed this activity for…

  8. An Inquiry into Juan Ramon's Interest in Walter Pater

    Directory of Open Access Journals (Sweden)

    John C. Wilcox

    1983-01-01

    Full Text Available The evidence for Juan Ramon's interest in Pater, which began around 1920 and was still active twenty years later, is discussed in this paper. Pater's view of death and dying and his attitude toward the decadent persona are described in so far as they indicate the spiritual affinity that exists between him and Juan Ramón. Pater's aesthetic idealism, and the presence of similar ideals in Juan Ramon's own work are then examined. The second part of the paper concentrates on the great interest Juan Ramón took in Pater's evocation of the Mona Lisa. The potential impact of the aesthetic idealism inherent in this passage, its Platonism in particular, receives special analysis in the light of Espacio , and in consideration of Juan Ramon's 1943 remark: "I am, have been, and always will be a Platonist."

  9. JUAN SOLDADO, PROTECTOR SOBRENATURAL DE LOS MIGRANTES

    OpenAIRE

    González Pérez, Cándido; Reynoso Rábago, Alfonso; Universidad de Guadalajara

    2013-01-01

    Los trabajadores mexicanos han emigrado a Estados Unidos de América desde mediados del siglo XIX y como producto de la profunda tradición religiosa heredada de España, han buscado apoyo sobrenatural para lograr sus objetivos: cruzar la frontera de manera ilegal y obtener un puesto de trabajo que haga posible enviar recursos económicos a sus familiares. En este tenor se creó un “protector sobrenatural inédito”:Juan Soldado quien en vida hab...

  10. Programa educativo del Infante D. Juan Manuel

    OpenAIRE

    Sánchez, Marciano

    1986-01-01

    RESUMEN: Si hemos de creer al Prólogo-Dedicatoria, el infante D. JUAN MANUEL parece que se sentía muy preocupado por los problemas de su tiempo, hasta el punto de que le hacían «perder el dormir». Como terapia contra este insomnio utilizaba el leer y escribir. En consecuencia, si el origen de este Libro del cauallero et del escudero, calificado por Díaz Plaja como «curiosa enciclopedia didáctica de su tiempo», hay que buscarlo en las noches de insomnio que padecía en Sevilla, es lícito conclu...

  11. 33 CFR 165.776 - Security Zone; Coast Guard Base San Juan, San Juan Harbor, Puerto Rico

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Security Zone; Coast Guard Base San Juan, San Juan Harbor, Puerto Rico 165.776 Section 165.776 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY REGULATED NAVIGATION AREAS AND LIMITED ACCESS AREAS Specific...

  12. Development of a microplate coagulation assay for Factor V in human plasma

    Directory of Open Access Journals (Sweden)

    Samis John A

    2011-06-01

    Full Text Available Abstract Background Factor V (FV in its activated form, FVa, is a critical regulator of thrombin generation during fibrin clot formation. There is a need of a simple, fast, and inexpensive microplate-based coagulation assay to measure the functional activity of FV in human plasma. The objective of this study was to develop a microplate-based assay that measures FV coagulation activity during clot formation in human plasma, which is currently not available. Methods The FV assay requires a kinetic microplate reader to measure the change in absorbance at 405nm during fibrin formation in human plasma. The FV assay accurately measures the time, initial rate, and extent of fibrin clot formation in human plasma. Results The FV microplate assay is simple, fast, economical, sensitive to approx 24-80pM, and multiple samples may be analyzed simultaneously. All the required materials are commercially available. Standard curves of time or initial rate of fibrin clot formation vs FV activity in the 1-stage assay (Without activation by thrombin may be used to measure FV activity in samples of human plasma. The assay was used to demonstrate that in nine patients with disseminated intravascular coagulation (DIC, the FV 1-stage, 2-stage (With activation by thrombin, and total (2-stage activity - 1-stage activity activities were decreased, on average, by approximately 54%, 44%, and 42%, respectively, from prolonged clot times when compared to normal pooled human reference plasma (NHP. The results indicate that the FV in the DIC patient plasmas supported both a delayed and slower rate of fibrin clot formation compared with NHP; however, the extent of fibrin clot formation in the DIC patients remained largely unchanged from that observed with NHP. Conclusions The FV microplate assay may be easily adapted to measure the activity of any coagulation factor using the appropriate factor-deficient plasma and clot initiating reagent. The microplate assay will find use in

  13. Measurement of factor v activity in human plasma using a microplate coagulation assay.

    Science.gov (United States)

    Tilley, Derek; Levit, Irina; Samis, John A

    2012-09-09

    In response to injury, blood coagulation is activated and results in generation of the clotting protease, thrombin. Thrombin cleaves fibrinogen to fibrin which forms an insoluble clot that stops hemorrhage. Factor V (FV) in its activated form, FVa, is a critical cofactor for the protease FXa and accelerator of thrombin generation during fibrin clot formation as part of prothrombinase (1, 2). Manual FV assays have been described (3, 4), but they are time consuming and subjective. Automated FV assays have been reported (5-7), but the analyzer and reagents are expensive and generally provide only the clot time, not the rate and extent of fibrin formation. The microplate platform is preferred for measuring enzyme-catalyzed events because of convenience, time, cost, small volume, continuous monitoring, and high-throughput (8, 9). Microplate assays have been reported for clot lysis (10), platelet aggregation (11), and coagulation Factors (12), but not for FV activity in human plasma. The goal of the method was to develop a microplate assay that measures FV activity during fibrin formation in human plasma. This novel microplate method outlines a simple, inexpensive, and rapid assay of FV activity in human plasma. The assay utilizes a kinetic microplate reader to monitor the absorbance change at 405 nm during fibrin formation in human plasma (Figure 1) (13). The assay accurately measures the time, initial rate, and extent of fibrin clot formation. It requires only μl quantities of plasma, is complete in 6 min, has high-throughput, is sensitive to 24-80 pM FV, and measures the amount of unintentionally activated (1-stage activity) and thrombin-activated FV (2-stage activity) to obtain a complete assessment of its total functional activity (2-stage activity - 1-stage activity). Disseminated intravascular coagulation (DIC) is an acquired coagulopathy that most often develops from pre-existing infections (14). DIC is associated with a poor prognosis and increases mortality

  14. Juan Antonio Rubio Rodriguez (1944 – 2010)

    CERN Multimedia

    His colleagues and friends

    2010-01-01

    It was with deep sorrow and great sadness that we learnt that Juan Antonio Rubio Rodriguez had passed away on 16th January 2010. Juan Antonio was born in Madrid on 4th June 1944, and received his Ph. D. in Physics in 1971 from“Universidad Complutense de Madrid”. He was a CERN Fellow (1968 – 1971) and subsequently worked at JEN (currently CIEMAT) as a researcher (1971 – 1976). He was leader of the HEP group (1977 – 1981), leader of the Nuclear and Particle Physics Division (1981 – 1983), Director for Basic Research (1983 – 1987) and Scientific Director (1984 – 1987). He was instrumental in the Spanish accession to CERN approved by the Spanish Government at the end of 1982 and ratified by the Spanish Parliament in June 1983. He served at CERN (1987 – 2004) as Group Leader (1987 – 1990), Scientific Advisor to the Director-General (1990 – 2000) and as Division Leader of the Education and Technology T...

  15. Programa educativo del Infante D. Juan Manuel

    Directory of Open Access Journals (Sweden)

    Marciano SÁNCHEZ

    2010-03-01

    Full Text Available RESUMEN: Si hemos de creer al Prólogo-Dedicatoria, el infante D. JUAN MANUEL parece que se sentía muy preocupado por los problemas de su tiempo, hasta el punto de que le hacían «perder el dormir». Como terapia contra este insomnio utilizaba el leer y escribir. En consecuencia, si el origen de este Libro del cauallero et del escudero, calificado por Díaz Plaja como «curiosa enciclopedia didáctica de su tiempo», hay que buscarlo en las noches de insomnio que padecía en Sevilla, es lícito concluir que los temas educativos constituían una preocupación para el Infante. Si bien no es tanto un tratado sistemático de educación cuanto una «fabliella» —género literario de la época que funde lo novelesco y lo didáctico— da pautas suficientes para, de una parte, deducir cuál sería el método de educación imperante entre los nobles junto con sus principales contenidos educativos, y, de otra, inferir el sistema ideal para don Juan Manuel.

  16. Investigation of the nonlinear static and dynamic behaviour of rectangular microplates under electrostatic actuation

    KAUST Repository

    Saghir, S.

    2016-11-16

    We present an investigation of the static and dynamic behavior of the nonlinear von-Karman plates when actuated by the nonlinear electrostatic forces. The investigation is based on a reduced order model developed using the Galerkin method, which rely on modeshapes and in-plane shape functions extracted using a finite element method. In this study, a fully clamped microplate is considered. We investigate the static behavior and the results are validated by comparison with the results calculated by a finite element model. The forced-vibration response of the plate is then investigated when the plate is excited by a harmonic AC load superimposed to a DC load. The dynamic behavior is examined near the primary resonance. The microplate shows a strong hardening behavior due to the cubic nonlinearity of mid-plane stretching. However, the behavior switches to softening as the DC load is increased.

  17. Microplate Ames MPF™ test use in assessment of mutagenic properties of dust pollution

    Directory of Open Access Journals (Sweden)

    Agnieszka Kozłowska

    2012-09-01

    Full Text Available Background: Highly industrialized Upper Silesia Region is particularly polluted by both anthropogenic and natural airborne particulate matters, which may lead to negative health effects in human. Materials and methods: The aim of the study was to assess the mutagenic properties of dust extracts which were collected in six cities in the Silesian Voivodeship. Dust samples were collected on glass fiber filters by the aspirator with air flow ca. 1 m3/min. Extraction of pollution was carried out using dichlorometane. The extracted samples were dissolved in dimethylsulfoxide (DMSO. The mutagenic properties were assessed using microplate Ames assay MPFTM with the use of bacteria Salmonella typhimurium strain TA98 and TA100. Results: In microplate Ames assay MPFTM there was observed a linear dose-response relationship in both metabolic variants of TA98 strain. Similar relationship was observed for TA100 strain with metabolic activation (S9. Mutagenic activity (AM of 100% extracts for TA98 strain in both metabolic variants (S9 exceeded 2, what indicate highly mutagenic effects of dust extracts. There was no mutagenic activity observed in the assay with TA100 (S9, AM 1. In the variant with exogenous metabolic activation (S9 in TA100 strain AM values ranged from AM1,160,15 to AM9,671,02. Mutagenic activity varied between different cities. Conclusions: The study demonstrated that microplate Ames assay MPFTM is fast and complex method of assessing the mutagenic properties of dust pollution, which exert toxic effect on organisms. The use of microplate Ames assay MPFTM together with chemical analyses of air dust pollution may evaluate the level of exposure in the environment and enable to perform health risk assessment in populations exposed to mutagenic, toxic and cytotoxic substances.

  18. Brunn: An open source laboratory information system for microplates with a graphical plate layout design process

    OpenAIRE

    Larsson Rolf; Spjuth Ola; Andersson Claes; Alvarsson Jonathan; Wikberg Jarl ES

    2011-01-01

    Abstract Background Compound profiling and drug screening generates large amounts of data and is generally based on microplate assays. Current information systems used for handling this are mainly commercial, closed source, expensive, and heavyweight and there is a need for a flexible lightweight open system for handling plate design, and validation and preparation of data. Results A Bioclipse plugin consisting of a client part and a relational database was constructed. A multiple-step plate ...

  19. Presencia de microplásticos en cuatro playas arenosas de Perú

    Directory of Open Access Journals (Sweden)

    Sara Purca

    2017-04-01

    Full Text Available Se presenta el número y peso por metro cuadrado de fragmentos de microplásticos presentes en cuatro playas arenosas de la costa peruana. Las muestras fueron colectadas entre junio de 2014 y mayo 2015. Fragmentos de plástico duro mayores a 1 mm fueron encontrados en más del 80% de las muestras de las cuatro playas. La playa Costa Azul (~ 12°S presentó 522 fragmentos por metro cuadrado (items/m2 de microplásticos, de los cuales, 463.33 items/m2 y 2.6 g/m2 fueron plásticos duros. La playa Albúfera de Medio Mundo (~ 11°S presentó el menor número y peso de microplásticos (4.67 items/m2 y 0.50 g/m2. Una muestra aleatoria de la playa Costa Azul fue analizada por el espectroscopio FT-IR. Se encontró 5 fragmentos con poliuretano (PE, dos fragmentos con polipropileno (PP y un fragmento con estireno (EPS. Pocos estudios mencionan al plástico duro como la mayor fracción de los microplásticos, se sugiere nuevas fuentes de intrusión y vías de microbasura en los hábitas, los cuales podrían estar afectando desde la base de trama trófica marina en el Perú.

  20. Micro-plate magnetic chemiluminescence immunoassay and its applications in carcinoembryonic antigen analysis

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    A micro-plate magnetic chemiluminescence immunoassay was developed for rapid and high throughput detection of carcinoembryonic antigens (CEA) in human sera. This method was based on a sandwich immunoreaction of fluorescein isothiocyanate (FITC)-labeled anti-CEA antibodies, CEA antigens, and horseradish peroxidase (HRP)-conjugated anti-CEA antibodies in mi- cro-plate. The immunomagnetic particles coated with anti-FITC antibodies were used as the solid phase for the immunoassay. The separation procedure was carried out by a magnetic plate adaptor and the luminol-hydrogen peroxide (H2O2)-HRP system was employed for the chemiluminescence detection. The proposed method combined the advantages of the micro-plate reactor and magnetic particle separation technology with the linear range of 5-250 ng mL·1. The detection limit of CEA was 0.61 ng mL·1. The coefficient of the variation was less than 7% and 13% for intra-assay and inter-assay precision, respectively. Compared with the commercial micro-plate chemiluminescent kit, the proposed method showed a good correlation.

  1. An investigation of the static and dynamic behavior of electrically actuated rectangular microplates

    KAUST Repository

    Saghir, S.

    2016-06-16

    We present an investigation of the static and dynamic behavior of the nonlinear von-Karman plates when actuated by the nonlinear electrostatic forces. The investigation is based on a reduced order model developed using the Galerkin method, which rely on modeshapes and in-plane shape functions extracted using a finite element method. In this study, a fully clamped microplate is considered. We investigate the static behavior and the effect of different non-dimensional design parameters. The static results are validated by comparison with the results calculated by a finite element model. The forced-vibration response of the plate is then investigated when the plate is excited by a harmonic AC load superimposed to a DC load. The dynamic behavior is examined near the primary and secondary (superharmonic and subharmonic) resonances. The microplate shows a strong hardening behavior due to the cubic nonlinearity of mid-plane stretching. However, the behavior switches to softening as the DC load is increased. Finally, near-square plates are studied to understand the effect of geometric imperfections of microplates.

  2. Application of a microplate scale fluorochrome staining assay for the assessment of viability of probiotic preparations.

    Science.gov (United States)

    Alakomi, H-L; Mättö, J; Virkajärvi, I; Saarela, M

    2005-07-01

    Cell viability in probiotic preparations is traditionally assessed by the plate count technique. Additionally, fluorescent staining combined with epifluorescence microscopy or flow cytometry has been developed for the viability assessment, but the currently available assays are either laborious or require highly sophisticated equipment. The aim of this study was to investigate the applicability of a microplate scale fluorochrome assay for predicting the cell state of freeze-dried Lactobacillus rhamnosus and Bifidobacterium animalis subsp. lactis preparations. In addition to viability assessment with LIVE/DEAD BacLight Bacterial Viability Kit, DiBAC(4)3 stain was used for the kinetic measurement of changes in bifidobacterial cell membrane functions during exposure to low pH. The microplate scale fluorochrome assay results on the viability and cell numbers of probiotic preparations correlated well with the results obtained with the culture-based technique and (with few exceptions) with epifluorescence microscopy. The assay was applicable also for the viability assessment of stressed (acid-treated) cells provided that the cell density in treatments was adjusted to the optimal measurement level of the fluorometer. The microplate scale fluorochrome assay offers a rapid and robust tool for the viability assessment of probiotic preparations, and enables also kinetic measurements.

  3. Nova Scotia Power response to Hurricane Juan

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2003-10-01

    Hurricane Juan hit the Halifax Regional Municipality on September 28, 2003, creating the largest outage in Nova Scotia Power's history. This detailed report documents the extensive damage that Hurricane Juan caused to the power transmission and distribution system in Nova Scotia. It also reviews the massive power restoration effort, with reference to numerous interviews, computer records and data logs which offer a wide range of observations, statistics and insights into the preparation and performance of Nova Scotia Power Inc. (NSPI) and the efforts of other key organizations following the storm. NSPI organized a recovery effort that matched the intensity of the hurricane. A fire in the Scotia Square Office Tower caused the evacuation of the company's call centre. The Tufts Cove station in Dartmouth, which generates 400 megawatts of power, was forced to shut down. Excess electricity was moved into New Brunswick and other jurisdictions to maintain system stability. The main priority was to restore customers back to service. Within 5 days of the hurricane, 95 per cent of those who lost power had service restored. Hurricane Juan caused the most damage to the transmission and distribution system in NSPI's history. Three out of five high capacity transmission lines were put out of service. Three 120-foot high transmission towers fell, and 17 main transmission lines were damaged and put out of service. Forty-five major substations were affected and 145 distribution feeders were damaged or tripped off, including 106 in the Halifax Regional Municipality. Large portions of 4,500 kilometres of local distribution lines in the Halifax Regional Municipality were damaged, including thousands of kilometers across the Northeast. The power crew, consisting of 2,000 individuals from the region and neighbouring utilities in New Brunswick and Maine, worked for 15 consecutive days to replace 275 transformers, 760 power poles, and 125,000 metres of conductor wire. NSPI

  4. Un San Juan Bautista firmado por Juan Simón Gutiérrez

    Directory of Open Access Journals (Sweden)

    Requena Bravo de Laguna, José Luis

    2003-12-01

    Full Text Available El conocimiento de la pintura sevillana de la segunda mitad del siglo XVII supone uno de los grandes retos de nuestra historiografía artística. Efectivamente, el amplio abanico de discípulos e imitadores del arte de Murillo necesita una profunda revisión metodológica que sepa clarificar la nada fácil tarea de aislar las distintas personalidades que constituyen este importante grupo de artistas. En este sentido la personalidad de Juan Simón Gutiérrez (h. 1634-1724 continua siendo bastante confusa si bien con algunos interrogantes se le van atribuyendo ciertas obras que puedan responder al todavía escaso conocimiento de su estilo frente al amplio grupo de pintores pertenecientes a la escuela de Murillo. Este inédito San Juan Bautista predicando en el desierto (fig. 1, firmado por Juan Simón Gutiérrez (figs. 2-3 procedente de una colección cordobesa…

  5. Virgilio según Juan Pablo II

    Directory of Open Access Journals (Sweden)

    Antonio Arbea

    2003-09-01

    Full Text Available El año 1981, con motivo de la conmemoración de los dos mil años de lamuerte de Virgilio, el Papa Juan Pablo II pronunció un ilustrado discurso, en latín, en torno a la figura y la obra del poeta latino. En este artículo se traducen y comentan algunos pasajes destacados de esta singular pieza oratoria. (In 1981, Pope John Paul II delivered an enlightened speech in Latin about poet Virgil’s work and figure, to commemorate the 2000th anniversary of his death. This article translates and comments on some selected passages of this uncommon piece of oratory.

  6. Juan Ruiz político

    OpenAIRE

    Georges Martin

    2007-01-01

    Más bien que en la vida amorosa del Arcipreste de Hita, de la que no sabemos nada, quizá tengamos que ver en el importantísimo conflicto con incidencias dinásticas virtualmente devastadoras entre María de Portugal, la mujer legítima de Alfonso XI, y Leonor de Guzmán, su concubina oficial, o, si se quiere, en el “loco amor” del rey y en el partido (el de María) que –sugiero- escogió Juan Ruiz, la causa del encarcelamiento del autor del Libro de buen amor. También se comprendería que dicho cont...

  7. Multi-mode conversion imaging of the subducted Gorda and Juan de Fuca plates below the North American continent

    Science.gov (United States)

    Tauzin, Benoit; Bodin, Thomas; Debayle, Eric; Perrillat, Jean-Philippe; Reynard, Bruno

    2016-04-01

    Receiver function analysis and seismic tomography show tectonic structures dipping eastward in the mantle below the Cascadia volcanic arc (western US) that have been related to the subduction of the Gorda and Juan de Fuca oceanic micro-plates. Inconsistencies in the dip angle and depth extent of the slab between the two methods undermine the interpretation of the structure and processes at work. Receiver function imaging is biased by multiple reflection phases that interfere with converted phases, and produce spurious discontinuities in images. Here, we correct the interference using a multiple mode conversion imaging technique that efficiently removes artifacts under dipping structures. The method has the advantage of being applicable to large aperture arrays, and can image large-scale structures down to the transition zone. With this approach, the interfaces between the subducting and overriding plates and the oceanic Moho are imaged at shallow depths (<120 km) with a dip angle of ∼20°, consistently with former studies. In addition, several important features are imaged with the present method. Faint converters located between 100 and 400 km depth in the mantle wedge, and strong sub-horizontal seismic scatterers near 160 km depth, may highlight dehydration and metasomatism processes in the Cascadia subduction zone. A discontinuity located at ∼15 km depth in the lithospheric mantle of the subducted plates and associated with a negative impedance contrast is interpreted as the fossil fabric of the plates acquired at the spreading ridges.

  8. San Juan, Puerto Rico Tsunami Forecast Grids for MOST Model

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The San Juan, Puerto Rico Forecast Model Grids provides bathymetric data strictly for tsunami inundation modeling with the Method of Splitting Tsunami (MOST) model....

  9. Strait of Juan de Fuca 1 arc-second DEM

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The 1-second Strait of Juan de Fuca Washington Elevation Grid provides bathymetric data in ASCII raster format of 1-second resolution in geographic coordinates. This...

  10. Strait of Juan de Fuca 6 arc-second DEM

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The 6-second Strait of Juan de Fuca Washington Elevation Grid provides bathymetric data in ASCII raster format of 6-second resolution in geographic coordinates. This...

  11. Strait of Juan de Fuca 36 arc-second DEM

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The 36-second Strait of Juan de Fuca Washington Elevation Grid provides bathymetric data in ASCII raster format of 36-second resolution in geographic coordinates....

  12. Un nuevo retrato de Juan Pantoja de la Cruz

    Directory of Open Access Journals (Sweden)

    de la Torre Fazio, Julia

    2010-06-01

    Full Text Available The author attributes an early 17th century Spanish portrait of an unknown young lady in a French private collection to Juan Pantoja de la Cruz.

    Atribución de un retrato español de joven dama desconocida de principios del siglo XVII, conservado en una colección particular francesa, a Juan Pantoja de la Cruz.

  13. Multiwell Ojo Alamo development advancing in San Juan basin

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-04-27

    Commercial production from a new formation is rare in a basin as mature as the San Juan. Such a development can be economically attractive because with so many existing wellbores, behind pipe formations can be placed on production quickly and inexpensively. That is happening on the east side of the San Juan, where what appears to be the first significant commercial gas production from Tertiary Ojo Alamo sandstone started last year. The deposit is briefly described.

  14. Juan-Ron fever: A rare case report.

    Science.gov (United States)

    Acharya, Sourya; Shukla, Samarth

    2015-01-01

    Juan-Ron fever named after Juan Rosai and Ronald Dorfman is the fever associated with Rosai-Dorfman disease also known as sinus histiocytosis with massive lymphadenopathy (SHML). It is a rare disorder of unknown etiology that is characterized by abundant macrophages in the lymph nodes throughout the body. Usually patient presents with painless lymphadenopathy. We present a case of a 45-year-old male who presented to us with bilateral cervical lymphadenopathy and fever, later on diagnosed to have SHML.

  15. An experimental and theoretical investigation of the mechanical behavior of multilayer initially curved microplates under electrostatic actuation

    KAUST Repository

    Saghir, Shahid

    2017-04-07

    We investigate the static and dynamic behavior of a multilayer clamped-free-clamped-free (CFCF) microplate, which is made of polyimide, gold, chromium, and nickel. The microplate is slightly curved away from a stationary electrode and is electrostatically actuated. The free and forced vibrations of the microplate are examined. First, we experimentally investigate the variation of the first natural frequency under the electrostatic DC load. Then, the forced dynamic behavior is investigated by applying a harmonic AC voltage superimposed to a DC voltage. Results are shown demonstrating the transition of the dynamic response of the microplate from hardening to softening as the DC voltage is changed as well the dynamic pull-in phenomenon. For theoretical model, we adopt a dynamic analog of the von-Karman governing equations accounting for initial curvature imperfection. These equations are then used to develop a reduced order model based on the Galerkin procedure to simulate the mechanical behavior of the microplate. We compare the theoretical results with experimental data and show excellent agreement among the results. We also examine the effect of the initial rise on the natural frequencies of first three symmetric-symmetric modes of the plate.

  16. The August 2002 earthquake sequence in north Afar: Insights into the neotectonics of the Danakil microplate

    Science.gov (United States)

    Ayele, Atalay; Stuart, Graham; Bastow, Ian; Keir, Derek

    2007-06-01

    In August 2002, there was high seismic activity in Afar concentrated at the plateau margin of the northern Ethiopian rift east of Mekele, near the western part of the Danakil microplate. The spatial and temporal distributions of this seismic activity over four weeks indicate the NNW propagation of the Gulf of Aden rift across the Afar Depression towards the western Ethiopian plateau. Fault plane solutions for six larger earthquakes from the August 2002 sequence are estimated from moment tensor inversion of local broadband waveform data. The results show only normal faulting on NNW trending and NE dipping faults, which agree with tectonics of the area and distribution of aftershocks. No strike-slip component is observed in any of our fault plane solutions or those of other workers including Harvard CMT solutions in the region. Such motion would be indicative of oblique-slip deformation between the Nubian plate and the Danakil microplate consistent with counter-clockwise rotation of the microplate. Hypocentral depths of well-constrained events are 5-7 km, which is the approximate elastic plate thickness in the Main Ethiopian rift, possibly indicating the depth to the brittle-ductile transition zone in this part of the Afar Depression. The shallowness of the depth estimates agree with the macroseismic reports available from a wide area in northern Ethiopia. Potential future shallow crustal deformation may cause significant loss of human life and damage to property in the densely populated highland region around Mekele unless measures are taken in improving building standards. The b-value for this sequence is estimated to be 0.66 using a least squares fit, while it is 0.67 ± 0.16 from a maximum-likelihood approach. This estimated b-value is low or the frequency of occurrence of relatively larger magnitude events is high indicating that it is a highly stressed region as evidenced by the recent increase of the seismicity in the area.

  17. Characterization of growth inhibition of oral bacteria by sophorolipid using a microplate-format assay.

    Science.gov (United States)

    Solaiman, Daniel K Y; Ashby, Richard D; Uknalis, Joseph

    2017-05-01

    Sophorolipid (SL) is a class of glycolipid biosurfactant produced by yeast and has potent antimicrobial activity against many microorganisms. In this paper, a microplate-based method was developed to characterize the growth inhibition by SL on five representative species of caries-causing oral bacteria. Bacterial growth on microplate in the absence and presence of varying concentrations of SL was continuously monitored by recording the absorbance at 600nm of the cultures using a microplate reader. The results showed that SL completely inhibited the growth of the Lactobacilli at ≥1mg/ml and the Streptococci at much lower concentrations of ≥50μg/ml. More importantly, we further defined the mechanism of antimicrobial activity of SL by analyzing the pattern of the cell growth curves. SL at sublethal concentrations (<1mg/ml) is bactericidal towards the Lactobacilli; it lengthens the apparent cell-doubling time (Td) and decreases the final cell density (as indicated by A600nm) in a concentration-dependent manner. Against the oral Streptococci, on the other hand, SL at sublethal concentrations (<50μg/ml) is bacteriostatic; it delays the onset of cell growth in a concentration-dependent fashion, but once the cell growth is commenced there is no noticeable adverse effect on Td and the final A600nm. Scanning electron microscopic (SEM) study of L. acidophilus grown in sublethal concentration of SL reveals extensive structural damage to the cells. S. mutans grown in sublethal level of SL did not show morphological damage to the cells, but numerous protruding structures could be seen on the cell surface. At the respective lethal levels of SL, L. acidophilus cells were lysed (at 1mg/ml SL) and the cell surface structure of S. mutans (at 130μg/ml SL) was extensively deformed. In summary, this paper presents the first report on a detailed analysis of the effects of SL on Lactobacilli and Streptococci important to oral health and hygiene.

  18. Juan Ruiz político

    Directory of Open Access Journals (Sweden)

    Georges Martin

    2007-01-01

    Full Text Available Más bien que en la vida amorosa del Arcipreste de Hita, de la que no sabemos nada, quizá tengamos que ver en el importantísimo conflicto con incidencias dinásticas virtualmente devastadoras entre María de Portugal, la mujer legítima de Alfonso XI, y Leonor de Guzmán, su concubina oficial, o, si se quiere, en el “loco amor” del rey y en el partido (el de María que –sugiero- escogió Juan Ruiz, la causa del encarcelamiento del autor del Libro de buen amor. También se comprendería que dicho contexto haya dado lugar a una obra que, tan ortodoxa en su sententia como genialmente paradójica en su littera, no tergiversa sobre los fundamentos a la vez sociales y espirituales del “buen amor” como tampoco se muestra muy propicia a la realeza tal y como la encarnaba entonces Alfonso XI de Castilla.Plutôt que dans la vie amoureuse de l’Archiprêtre de Hita, dont nous ne savons rien, peut-être faudrait-il voir dans le grave conflit aux incidences dynastiques virtuellement dévastatrices qui opposa Marie de Portugal, femme légitime d’Alphonse XI, et Aliénor de Guzmán, sa concubine officielle, ou, si l’on veut, dans le « fol amour » du roi et dans le parti (celui de Marie que choisit –je le suggère– Juan Ruiz, la cause de l’emprisonnement de l’auteur du Libro de buen amor. L’on comprendrait aussi que ce contexte ait donné lieu à une œuvre qui, aussi orthodoxe dans sa sententia que génialement paradoxale dans sa littera, ne tergiverse nullement sur les fondements sociaux et spirituels du « bon amour » mais ne se montre pas non plus favorable à une royauté telle que l’incarne alors Alphonse XI de Castille.

  19. Las Silvas de Juan Lorenzo Palmireno

    Directory of Open Access Journals (Sweden)

    Lee, Lilith

    2006-12-01

    Full Text Available This paper examines the works titled Silva by Juan Lorenzo Palmireno. The analysis of these texts sheds light on Palmireno’s understanding of the word and its possible implications in the sixteenth century. The study identifies an interpretation of silva as a way of writing different from his predecessor, Pedro Mexía, who first used it to title his work Silva de varia lección twenty years ago. The lack of any intention to arrange its structure gives these silvas the characteristic of a literary draft, a feature derived from one of the meanings of silva inherited from the Classical Age. Moreover, Palmireno’s works reveal an encyclopedic project of which the miscellany (represented by silva forms a part. Furthermore, the silvas of Palmireno act as a transition to the later development of silva as a genre, which finds its educational objective being taken over gradually by the anxiety to provide entertainment.El artículo examina las obras tituladas Silva de Juan Lorenzo Palmireno. El análisis de estos textos deja constancia de cómo el humanista entiende la palabra y sus implicaciones en el siglo XVI. El estudio identifica una interpretación de silva como una manera de escribir distinta de su predecesor, Pedro Mexía, quien veinte años antes la utilizó para nombrar a su obra Silva de varia lección. La falta de la intención en ordenar los textos les otorga a las silvas palmirenianas la característica de un borrador literario, uno de los significados de la silva desde la Edad Clásica. Además, las obras del humanista aragonés muestra su proyecto enciclopédico del autor que integra la miscelánea (representada por la silva como una parte. Estas silvas también actúan como la transición para el desarrollo de la silva como género que encuentra su objetivo educacional sustituido gradualmente por la finalidad de ofrecer entretenimiento.

  20. Effect of Residual Stress on Divergence Instability of Rectangular Microplate Subjected to Nonlinear Electrostatic Pressure

    Directory of Open Access Journals (Sweden)

    Ghader Rezazadeh

    2007-07-01

    Full Text Available In this paper, the effect of residual stress on divergence instability of a rectangular microplate subjected to a nonlinear electrostatic pressure for different geometrical properties has been presented. After deriving the governing equation and using of Step-by-Step Linearization Method (SSLM, the governing nonlinear equation has been linearized. By applying the finite difference method (FDM to a rectangular mesh, the linearized equation has been discretized. The results show, residual stresses have considerable effects on Pull-in phenomena. Tensile residual stresses increase pull-in voltage and compressive decrease it. The effect of different geometrical properties on divergence instability has also been studied.

  1. Juan Bautista Perolli. Obras genovesas. II

    Directory of Open Access Journals (Sweden)

    López Torrijos, Rosa

    2002-06-01

    Full Text Available In this article the study of Perolli's work in Genoa is continued, demonstrating his varied artistic activities and his relationship with families under Spanish influence. His last work in Genoa (the Spinola Chapel in the church of San Francisco had to be completed by other artists because Perolli was engaged by Don Alvaro de Bazán to work in his Spanish palace of El Viso. In December 1574, Bazán paid Perolli's debts and shortly afterwards Juan Bautista left for Spain.

    Continúa el estudio de la obra de Perolli en Génova, mostrando su participación en trabajos de arquitectura, escultura y pintura para varias familias genovesas, todas ellas relacionadas con España. Se estudia también su última obra realizada en Génova, la capilla Spinola de la iglesia de San Francisco, desaparecida y totalmente desconocida hasta ahora. Este último trabajo queda interrumpido en diciembre de 1574 cuando don Álvaro de Bazán paga las deudas de Perolli para que éste venga a España a trabajar en el palacio del Viso.

  2. JUAN SIN MIEDO VA A LA ESCUELA

    Directory of Open Access Journals (Sweden)

    Silvia Méndez-Anchía

    2009-01-01

    Full Text Available Tomando como base la lectura del cuento "Juan Sin Miedo" realizada por tres sujetos adolescentes, pretendo responder a la pregunta sobre los principales temores que enfrenta la población costarricense adolescente en la actualidad. Las respuestas giran en torno a tres núcleos significativos: el miedo a la exclusión económica y social, materializada en el trato discriminatorio hacia quienes no se adecuan a los criterios impuestos por la sociedad de consumo; el miedo a la escuela, en tanto excluye a quienes no se ajustan a la norma esperada de desempeño académico; y el miedo al otro, en particular a la calle y la escuela como espacios de violencia. Concluyo señalando que, detrás de dichos temores, es posible leer un anhelo de pertenencia; de aprecio sincero y gratuito, en que no medie el rendimiento académico de la persona; así como un anhelo de seguridad en el entorno cercano.

  3. Microplate Agglutination Test for Canine Brucellosis Using Recombinant Antigen-Coated Beads.

    Science.gov (United States)

    Castillo, Yussaira; Tachibana, Masato; Kimura, Yui; Kim, Suk; Ichikawa, Yasuaki; Endo, Yasuyuki; Watanabe, Kenta; Shimizu, Takashi; Watarai, Masahisa

    2014-01-01

    Brucella canis, a facultative intracellular pathogen, is the causative agent of canine brucellosis. The diagnosis of canine brucellosis is based on bacteriological examination and serological methods, including agglutination and gel diffusion tests. In this study, four recombinant antigens, heat shock protein 60, rhizopine-binding protein, Cu-Zn superoxide dismutase, and hypothetical protein (Ag 4), were constructed. These antigens were coated on latex beads and their usefulness in the serological diagnosis of canine brucellosis was examined. All recombinant antigens showed specific reaction with sera from B. canis-infected dogs in Western blotting. In a microplate agglutination test, mixing sera from B. canis-infected dogs, but not sera from B. canis-free dogs, with single or multiple antigens-coated latex beads produced clear agglutination. Moreover, the antigen-coated latex beads did not show nonspecific agglutination in hemolyzed serum samples. A survey of canine serum samples conducted by the microplate agglutination test using single antigen-coated latex beads showed that this method would be useful in the serological diagnosis of canine brucellosis. Further investigations using more serum samples are required to confirm the usefulness of our method.

  4. Antimicrobial properties of cyclodextrin-antiseptics-complexes determined by microplate laser nephelometry and ATP bioluminescence assay.

    Science.gov (United States)

    Finger, Susanne; Wiegand, Cornelia; Buschmann, Hans-Jürgen; Hipler, Uta-Christina

    2012-10-15

    Antimicrobial effects of substances can be determined with different methods that measure distinct parameters. Thus, a comparison of the results obtained can be difficult. In this study, two in vitro methods were employed to determine concentration and time dependent effects of cyclodextrin (CD)-complexes with the antiseptics chlorhexidine diacetate (CHX), iodine (IOD) and polihexanide (PHMB) on Candida albicans and Malassezia pachydermatis. Using both, microplate laser nephelometry and the ATP bioluminescence assay, it could be shown that CD-antiseptics-complexes tested exhibited significant antifungal effects with the exception of γ-CD-CHX in the case of C. albicans. Microplate laser nephelometry (MLN) is an optical method and enables a quantitative determination of particle concentrations in solution. By means of this method, microbial growth under influence of potential antimicrobial substances can be monitored over a prolonged time period. In addition, the antimicrobial activity was analyzed by measurement of the microbial adenosine triphosphate (ATP) content with a bioluminescent assay. The luminescent signal is directly proportional to the amount of ATP, and thus, a linear function of the number of living microbial cells present. Both methods were compared according to the half maximal inhibitory concentration (IC(50)) calculated and the statistical evaluation of Pearson's correlation coefficient (r). In summary, it could be demonstrated that both methods yield similar results although they differ in the parameter.

  5. Microplate-reader method for the rapid analysis of copper in natural waters with chemiluminescence detection

    Directory of Open Access Journals (Sweden)

    Axel eDurand

    2013-01-01

    Full Text Available We have developed a method for the determination of copper in natural waters at nanomolar levels. The use of a microplate-reader minimises sample processing time (~ 25 sec per sample, reagent consumption (~ 120 μL per sample and sample volume (~ 700 μL. Copper is detected by chemiluminescence. This technique is based on the formation of a complex between copper and 1,10-phenanthroline and the subsequent emission of light during the oxidation of the complex by hydrogen peroxide. Samples are acidified to pH 1.7 and then introduced directly into a 24-well plate. Reagents are added during data acquisition via two reagent injectors. When trace metal clean protocols are employed, the reproducibility is generally less then 7% on blanks and the detection limit is 0.7 nM for seawater and 0.4 nM for freshwater. More than 100 samples per hour can be analyzed with this technique, which is simple, robust, and amenable to at-sea analysis. Seawater samples from Storm Bay in Tasmania illustrate the utility of the method for environmental science. Indeed other trace metals for which optical detection methods exist (e.g. chemiluminescence, fluorescence and absorbance could be adapted to the microplate-reader.

  6. [Metabolic properties of the microbial community in the biofilters using biolog microplates].

    Science.gov (United States)

    Xi, Jin-Ying; Hu, Hong-Ying; Jiang, Jian; Qian, Yi

    2005-07-01

    It is very important to know the structure and metabolic function of the microbial community in a bioreactor in order to improve its performance. In this study, two biofilters, packed with wood chips and granular activated carbons respectively, were operated for 160 days to treat toluene gas. The metabolic profiles of the microbial communities in the biofilters were monitored using Biolog microplates periodically during the experiments. The metabolic activities of the microorganisms in both biofilters were observed to decrease during long-term operation. According to the results of principle components analysis, the metabolic profiles of the microbial communities did not change much in the former period of the operation, but they changed in the inlet layers on day 103 and changed throughout the filter beds on day 160. The variation of the metabolic profiles in both biofilters showed little difference, which suggested that the packing media had little effect on them during long-term operation. Among the 95 carbon sources in Biolog microplate, carboxylic acids and amino acids were much easier to be utilized by the microorganisms in the biofilters than the other carbon

  7. Metabolic activities of five botryticides against Botrytis cinerea examined using the Biolog FF MicroPlate.

    Science.gov (United States)

    Wang, Hancheng; Wang, Jin; Li, Licui; Hsiang, Tom; Wang, Maosheng; Shang, Shenghua; Yu, Zhihe

    2016-08-05

    Tobacco grey mold caused by Botrytis cinerea is an important fungal disease worldwide. Boscalid, carbendazim, iprodione, pyrimethanil and propiconazole are representative botryticides for grey mold management. This research investigated the sensitivities of B. cinerea from tobacco to these chemicals using the Biolog FF Microplate. All five chemicals showed inhibitory activity, with average EC50 values of 0.94, 0.05, 0.50, 0.61 and 0.31 μg ml(-1), respectively. B. cinerea metabolized 96.8% of tested carbon sources, including 29 effectively and 33 moderately, but the metabolic fingerprints differed under pressures imposed by these botryticides. For boscalid, B. cinerea was unable to metabolize many substrates related to tricarboxylic acid cycle. For carbendazim, carbon sources related to glycolysis were not metabolized. For iprodione, use of most carbon substrates was weakly inhibited, and the metabolic profile was similar to that of the control. For propiconazole, no carbon substrates were metabolized and the physiological and biochemical functions of the pathogen were totally inhibited. These findings provide useful information on metabolic activities of these botryticides, and may lead to future applications of the Biolog FF Microplate for examining metabolic effects of other fungicides on other fungi, as well as providing a metabolic fingerprint of B. cinerea that could be useful for identification.

  8. Rapid solid-phase immunoassay for 6-keto prostaglandin F1 alpha on microplates

    Energy Technology Data Exchange (ETDEWEB)

    Schramm, W.; Smith, R.H.; Jackson, T.M.; Craig, P.A.; Grates, H.E.; Minton, L.L. (BioQuant of Ann Arbor, Inc., MI (USA))

    1990-03-01

    We describe, for the measurement of 6-keto prostaglandin F1 alpha in biological media, a solid-phase immunoassay with immobilized antibodies that requires a total processing time of less than 2 h with hands-on time less than 30 min for 40 samples. The method combines the convenience of the microplate format with the sensitivity of radiolabeled prostaglandin derivatives as tracers in a competitive immunoassay. The intra- and interassay variations at 50% displacement of the radiolabeled prostaglandin derivative as tracer were 9.0% and 11.8%, respectively. At 50% displacement of the radiolabeled tracer, the sensitivity is about 20 pg per well. Optimal incubation time is between 60 and 90 min. Nonspecific binding was less than 1% if about 8 pg of tracer (approximately 25,000 counts/min per well) was used. Inhibition curves of samples in different dilutions were parallel to standard curves. The variation of bound radiolabeled prostaglandin derivative within the wells of one microplate (n = 96) was less than 3%. Human plasma samples and medium from tissue culture assayed for 6-keto prostaglandin F1 alpha correlated well with results obtained with a solid-phase assay based on use of magnetic particles (r = 0.99, n = 24) for culture-medium samples; r = 0.99; n = 26 for plasma samples.

  9. A paper/polymer hybrid microfluidic microplate for rapid quantitative detection of multiple disease biomarkers

    Science.gov (United States)

    Sanjay, Sharma T.; Dou, Maowei; Sun, Jianjun; Li, Xiujun

    2016-07-01

    Enzyme linked immunosorbent assay (ELISA) is one of the most widely used laboratory disease diagnosis methods. However, performing ELISA in low-resource settings is limited by long incubation time, large volumes of precious reagents, and well-equipped laboratories. Herein, we developed a simple, miniaturized paper/PMMA (poly(methyl methacrylate)) hybrid microfluidic microplate for low-cost, high throughput, and point-of-care (POC) infectious disease diagnosis. The novel use of porous paper in flow-through microwells facilitates rapid antibody/antigen immobilization and efficient washing, avoiding complicated surface modifications. The top reagent delivery channels can simply transfer reagents to multiple microwells thus avoiding repeated manual pipetting and costly robots. Results of colorimetric ELISA can be observed within an hour by the naked eye. Quantitative analysis was achieved by calculating the brightness of images scanned by an office scanner. Immunoglobulin G (IgG) and Hepatitis B surface Antigen (HBsAg) were quantitatively analyzed with good reliability in human serum samples. Without using any specialized equipment, the limits of detection of 1.6 ng/mL for IgG and 1.3 ng/mL for HBsAg were achieved, which were comparable to commercial ELISA kits using specialized equipment. We envisage that this simple POC hybrid microplate can have broad applications in various bioassays, especially in resource-limited settings.

  10. Revision of the genera Microplitis and Snellenius (Hymenoptera, Braconidae, Microgastrinae) from Area de Conservacion Guanacaste, Costa Rica, with a key to all species previously described from Mesoamerica

    Science.gov (United States)

    The genera Microplitis and Snellenius (Hymenoptera: Braconidae, Microgastrinae) from Area de Conservacion Guanacaste (ACG), Costa Rica, are revised. A total of 28 new species are described: 23 of Snellenius (the first record for Mesoamerica) and five of Microplitis. A key is provided to all new spec...

  11. Revised volcanic history of the San Juan, Uncompahgre, Silverton, and Lake City calderas in the western San Juan Mountains, Colorado

    Science.gov (United States)

    Lipman, Peter W.; Steven, Thomas A.; Luedke, Robert G.; Burbank, Wilbur

    1973-01-01

    The sequence of mid-Cenozoic volcanic events in the western San Juan Mountains is closely analogous to that elsewhere in the San Juan volcanic field. The Lake Fork, Picayune, and San Juan Formations were erupted from a cluster of central volcanoes from 35 to 30 m.y. ago, when dominant activity shifted to more silicic ash-flow eruptions with accompanying caldera collapses. The Uncompahgre and San Juan calderas, each about 20 km across, formed mainly from eruption of the 28-m.y.-old Sapinero Mesa Tuff. Collapse occurred concurrently with eruption, and intracaldera tuffs accumulated to a thickness of more than 700 m. Both calderas were resurgently domed together; the northeast-trending Eureka graben formed along the distended crest of that dome. The Uncompahgre caldera was then flooded by several 27- to 28-m.y.-old ash-flow sheets from easterly sources, and also by one apparently erupted from the Silverton caldera nested within the older San Juan caldera. The Lake City caldera, located within the older Uncompahgre caldera, formed about 22.5 m.y. ago in response to eruption of the Sunshine Peak Tuff.

  12. SMCRA bond release - the initial steps at San Juan mine

    Energy Technology Data Exchange (ETDEWEB)

    Estrada, O.; Clark, D. [BHP San Juan Coal Company, Waterflow, NM (United States)

    2001-07-01

    San Juan Coal Company has submitted to the State of New Mexico's Mining and Minerals Division the final phase III bond release application for the approval and release of 241 acres of permitted lands at its San Juan Mine located in San Juan County, New Mexico. The SMCRA (Surface Mining Control and Reclamation Act) and New Mexico Surface Coal Mining Regulations mandate a minimum 10-year liability period following final reclamation, during which no additional treatments other than approved postmining land uses and approved interseedings may be applied to an area for which bond release is sought. Site-specific revegetation success standards must be met for two of the last four years of liability, beginning no sooner than year eight of the bonding period. Successful reclamation of the site was demonstrated by exceeding cover, production, shrub density, and diversity standards while supporting the primary postmining land use of livestock grazing. 11 refs., 1 fig., 8 tabs.

  13. LOD First Estimates In 7406 SLR San Juan Argentina Station

    Science.gov (United States)

    Pacheco, A.; Podestá, R.; Yin, Z.; Adarvez, S.; Liu, W.; Zhao, L.; Alvis Rojas, H.; Actis, E.; Quinteros, J.; Alacoria, J.

    2015-10-01

    In this paper we show results derived from satellite observations at the San Juan SLR station of Felix Aguilar Astronomical Observatory (OAFA). The Satellite Laser Ranging (SLR) telescope was installed in early 2006, in accordance with an international cooperation agreement between the San Juan National University (UNSJ) and the Chinese Academy of Sciences (CAS). The SLR has been in successful operation since 2011 using NAOC SLR software for the data processing. This program was designed to calculate satellite orbits and station coordinates, however it was used in this work for the determination of LOD (Length Of Day) time series and Earth Rotation speed.

  14. Microplate-based platform for combined chromatin and DNA methylation immunoprecipitation assays

    Directory of Open Access Journals (Sweden)

    Yu Jingjing

    2011-11-01

    Full Text Available Abstract Background The processes that compose expression of a given gene are far more complex than previously thought presenting unprecedented conceptual and mechanistic challenges that require development of new tools. Chromatin structure, which is regulated by DNA methylation and histone modification, is at the center of gene regulation. Immunoprecipitations of chromatin (ChIP and methylated DNA (MeDIP represent a major achievement in this area that allow researchers to probe chromatin modifications as well as specific protein-DNA interactions in vivo and to estimate the density of proteins at specific sites genome-wide. Although a critical component of chromatin structure, DNA methylation has often been studied independently of other chromatin events and transcription. Results To allow simultaneous measurements of DNA methylation with other genomic processes, we developed and validated a simple and easy-to-use high throughput microplate-based platform for analysis of DNA methylation. Compared to the traditional beads-based MeDIP the microplate MeDIP was more sensitive and had lower non-specific binding. We integrated the MeDIP method with a microplate ChIP assay which allows measurements of both DNA methylation and histone marks at the same time, Matrix ChIP-MeDIP platform. We illustrated several applications of this platform to relate DNA methylation, with chromatin and transcription events at selected genes in cultured cells, human cancer and in a model of diabetic kidney disease. Conclusion The high throughput capacity of Matrix ChIP-MeDIP to profile tens and potentially hundreds of different genomic events at the same time as DNA methylation represents a powerful platform to explore complex genomic mechanism at selected genes in cultured cells and in whole tissues. In this regard, Matrix ChIP-MeDIP should be useful to complement genome-wide studies where the rich chromatin and transcription database resources provide fruitful foundation

  15. From a collage of microplates to stable continental crust - an example from Precambrian Europe

    Science.gov (United States)

    Korja, Annakaisa

    2013-04-01

    Svecofennian orogen (2.0-1.7 Ga) comprises the oldest undispersed orogenic belt on Baltica and Eurasian plate. Svecofennian orogenic belt evolved from a series of short-lived terrane accretions around Baltica's Archean nucleus during the formation of the Precambrian Nuna supercontinent. Geological and geophysical datasets indicate W-SW growth of Baltica with NE-ward dipping subduction zones. The data suggest a long-lived retreating subduction system in the southwestern parts whereas in the northern and central parts the northeasterly transport of continental fragments or microplates towards the continental nucleus is also documented. The geotectonic environment resembles that of the early stages of the Alpine-Himalayan or Indonesian orogenic system, in which dispersed continental fragments, arcs and microplates have been attached to the Eurasian plate margin. Thus the Svecofennian orogeny can be viewed as proxy for the initial stages of an internal orogenic system. Svecofennian orogeny is a Paleoproterozoic analogue of an evolved orogenic system where terrane accretion is followed by lateral spreading or collapse induced by change in the plate architecture. The exposed parts are composed of granitoid intrusions as well as highly deformed supracrustal units. Supracrustal rocks have been metamorphosed in LP-HT conditions in either paleo-lower-upper crust or paleo-upper-middle crust. Large scale seismic reflection profiles (BABEL and FIRE) across Baltica image the crust as a collage of terranes suggesting that the bedrock has been formed and thickened in sequential accretions. The profiles also image three fold layering of the thickened crust (>55 km) to transect old terrane boundaries, suggesting that the over-thickened bedrock structures have been rearranged in post-collisional spreading and/or collapse processes. The middle crust displays typical large scale flow structures: herringbone and anticlinal ramps, rooted onto large scale listric surfaces also suggestive

  16. Localized deformation zones in the offshore leading edge of the Yakutat microplate, Gulf of Alaska

    Science.gov (United States)

    Lowe, L. A.; Gulick, S. P.; Pavlis, T.; Bruhn, R. L.; Mann, P.

    2006-12-01

    The Gulf of Alaska margin is dominated by the collision and subduction of the Yakutat microplate as it travels northwest with respect to North America at near Pacific Plate velocities (\\~45 mm/yr). The oblique Yakutat block collision with North America is in transition between convergence to the west and translation along the Queen Charlotte-Fairweather-Denali Fault system to the east and north. Industry seismic reflection and high- resolution seismic reflection data collected by the R/V Maurice Ewing (2004) provides insight into how the Yakutat-North America collision is accommodated by active offshore structures near the leading edge of the Yakutat microplate. A \\~200 km wide area bounded by the Ten Fathom Fault, the offshore N. America-Yakutat contact, to the west and the eastern edge of the Pamplona Zone (PZ) to the east has previously been mapped as a continuous deformation zone consisting of NE-SW trending imbricate thrusts and folds. Though this mapping corroborates onshore measurements of active deformation west of the Bering Glacier in the Yakutat block, the relationship between current onshore deformation and the observed offshore structures remains unclear. Our observations indicate that neotectonic deformation is accommodated offshore by highly localized, asynchronous thrusts that, when analyzed in an accretionary context, may be connected by a sub-horizontal decollement. Data from the eastern edge of the PZ, the proposed deformation front, shows surface deformation caused by east-verging thrust faults. Seismic reflection profiles in the western PZ and the Bering Trough show no evidence of active tectonic deformation and up to \\~200 m of undisturbed sediments indicating that faulting in this part of the Yakutat block has been inactive since the Last Glacial Maximum or earlier. Farther west, above the Kayak Island fault zone, directly east of the Ten Fathom Fault, the presence of up to \\~50 m of undeformed sediments suggests a recent (ca. 14 ka

  17. Antimicrobial susceptibility of standard strains of nontuberculous mycobacteria by microplate Alamar Blue assay.

    Directory of Open Access Journals (Sweden)

    Guilian Li

    Full Text Available In this study, 24 standard nontuberculous mycobacteria (NTM species strains including 12 slowly growing mycobacteria strains and 12 rapidly growing mycobacteria strains were subjected to drug susceptibility testing using microplate Alamar Blue assay-based 7H9 broth. The most active antimicrobial agents against the 24 NTM strains were streptomycin, amikacin, the fluoroquinolones, and the tetracyclines. Mycobacterium chelonae, Mycobacterium abscessus, Mycobacterium bolletii, and Mycobacterium simiae are resistant to most antimicrobial agents. The susceptibility results of this study from 24 NTM standard strains can be referenced by clinicians before susceptibility testing for clinical isolates is performed or when conditions do not allow for susceptibility testing. The application of broth-based methods is recommended by the Clinical and Laboratory Standards Institute, and the documentation of the susceptibility patterns of standard strains of mycobacteria can improve the international standardization of susceptibility testing methods.

  18. Rapid microplate, green method for high-throughput evaluation of vinegar acidity using thermal infrared enthalpimetry.

    Science.gov (United States)

    Tischer, Bruna; Oliveira, Alessandra Stangherlin; Ferreira, Daniele de Freitas; Menezes, Cristiano Ragagnin; Duarte, Fábio Andrei; Wagner, Roger; Barin, Juliano Smanioto

    2017-01-15

    Infrared thermal imaging was combined with disposable microplates to perform enthalpimetric analysis using an infrared camera to monitor temperature without contact. The proposed thermal infrared enthalpimetry (TIE) method was used to determine the total, fixed and volatile acidities of vinegars. Sample preparation and analysis were performed in the same vessel, avoiding excessive sample handling and reducing energy expenditure by more than ten times. The results agreed with those of the conventional method for different kinds of vinegars, with values of 1.7%, and 2.3% for repeatability and intermediate precision, respectively. A linear calibration curve was obtained from 0.040 to 1.30molL(-1). The proposed method provided rapid results (within 10s) for four samples simultaneously, a sample throughput of up to 480 samples per hour. In addition, the method complies with at least eight of twelve recommendations for green analytical chemistry, making TIE a promising tool for routine vinegar analysis.

  19. Modal interactions in primary and subharmonic resonant dynamics of imperfect microplates with geometric nonlinearities

    Institute of Scientific and Technical Information of China (English)

    Hamed Farokhi; Mergen H Ghayesh

    2016-01-01

    This paper analyses the modal interactions in the nonlinear, size-dependent dynamics of geometrically imper-fect microplates. Based on the modified couple stress theory, the equations of motion for the in-plane and out-of-plane motions are obtained employing the von Kármán plate theory as well as Kirchhoff ’s hypotheses by means of the Lagrange equations. The equations of motions are solved using the pseudo-arclength continuation technique and direct time-integration method. The system parameters are tuned to the values associated with modal interactions, and then non-linear resonant responses and energy transfer are analysed. Nonlinear motion characteristics are shown in the form of frequency-response and force-response curves, time histo-ries, phase-plane portraits, and fast Fourier transforms.

  20. Typing dinucleotide repeat loci using microplate array diagonal gel electrophoresis: proof of principle.

    Science.gov (United States)

    Rodríguez, Santiago; Chen, Xiao-He; Day, Ian N M

    2004-04-01

    Polymorphic dinucleotide repeat loci ('microsatellite markers') are found in varying abundance throughout the genomes of most organisms. They have been extensively used for genetic studies, but conventional techniques used for their genotyping require sophisticated equipment. Microplate array diagonal gel electrophoresis (MADGE) has previously been extended to economical high-throughput genotyping of trinucleotide and tetranucleotide microsatellite amplicons. However, the capability of this technique to resolve the alleles of dinucleotide repeat loci has not been explored previously. Here we show that a modified microsatellite-MADGE approach can provide sufficient resolution for dinucleotide repeat typing. This enables economical and convenient set up for analysis of single markers in many samples in parallel, suitable, for example, for population association studies.

  1. Crustal Structure and Deformation of the Yakutat Microplate: New Insights From STEEP Marine Seismic Reflection Data

    Science.gov (United States)

    Lowe, L. A.; Gulick, S. P.; Christeson, G.; van Avendonk, H.; Reece, R.; Elmore, R.; Pavlis, T.

    2008-12-01

    In fall 2008, we will conduct an active source marine seismic experiment of the offshore Yakutat microplate in the northern Gulf of Alaska. The survey will be conducted aboard the academic research vessel, R/V Marcus Langseth, collecting deep-penetrating multi-channel seismic reflection survey using an 8-km, 640 channel hydrophone streamer and a 6600 cu. in., 36 airgun array. The survey is the concluding data acquisition phase for the ST. Elias Erosion and tectonics Project (STEEP), a multi-institution NSF-Continental Dynamics project investigating the interplay of climate and tectonics in the Chugach-St. Elias Mountains in southern Alaska. The experiment will also provide important site survey information for possible future Integrated Ocean Drilling Program investigations. Two profiles coincident with wide-angle refraction data (see Christeson, et al., this session) will image structural changes across the Dangerous River Zone from east to west and the Transition Fault from south to north. We will also image the western portion of the Transition Fault to determine the nature of faulting along this boundary including whether or not the Pacific Plate is underthrusting beneath the Yakutat microplate as part of this collision. Our westernmost profile will image the Kayak Island Zone, typically described as the northern extension of the Aleutian megathrust but which may be a forming suture acting as a deformation backstop for the converging Yakutat and North American plates. Profiles across the Pamplona Zone, the current Yakutat-North America deformation front, will further constrain relative timing of structural development and the depth of deformation on the broad folds and thrust faults that comprise the area. This new dataset will allow further insight into regional tectonics of the St. Elias region as well as provide more detail regarding the development of the south Alaskan margin during major Plio-Pleistocene glacial- interglacial periods.

  2. Entrevista a Juan Alberto Puyana, autor de El Preticante.

    OpenAIRE

    Carrión Jiménez, Andoni

    2012-01-01

    Juan Alberto Puyana es un tipo particular. Por su negra melena rizada y su perilla, propia del seguidor de Metallica que es, nadie diría que, además de enfermero, Alberto es un escritor con dos libros publicados.

  3. [Psychiatric Hospital San Juan de Dios. One hundred years later].

    Science.gov (United States)

    Cocula-León, Horacio

    2014-01-01

    Mental health and psychiatric diseases have always attracted people's and health authorities' attention due to its magical approach, the lack of knowledge that surrounds them, and, at the same time, the religious fear they provoke. Both have played an important role in the history of humanity, of public health politics, and of physicians. The places where psychiatric patients were treated are of historical interest, because through the historical knowledge we can identify an approach from the science and the health policies that prevailed in each age. At the beginning of the 20th century, it was developed in México a new model of hospital care attention to psychiatric patients. La Casa de Salud San Juan de Dios para Pacientes Alienados is an example; the concept "alienated patients" suggests a social and cultural perspective. This paper presents a chronological type description of one of the major institutions involved in mental health care in México. Similarly, it shows a review of the events that affected the religious order San Juan de Dios from 1901 to 2012, when the hospitaller order was reinstated in México and established the Casa de Salud San Juan de Dios para Pacientes Alienados in the town of Zapopan, Jalisco, institution that exists up to the present day and keeps participating in the mental health care in the state of Jalisco, with the current name of Servicios de Salud San Juan de Dios.

  4. Don Juan Huarte de San Juan: El doctor que anticipó la melancolía de Don Quijote Don Juan Huarte de San Juan: The doctor who antocipated Don Quixote´s Melancholy

    Directory of Open Access Journals (Sweden)

    Pedro García Martín

    2009-03-01

    Full Text Available El estado de enajenación del ingenioso hidalgo don Quijote lo había aprendido Cervantes a través del doctor Juan Huarte San Juan, autor del famoso ensayo Examen de ingenios, donde formula la teoría de los humores para explicar el cuerpo y el carácter del hombre. En este artículo, pues, repasaremos la biografía de este personaje, analizaremos la formación de los médicos en las universidades de la España del Siglo de Oro, la influencia del Examen en la Europa de su tiempo y en la obra cervantina a través de la melancolía de don Quijote y de la locura del Licenciado Vidriera. Para terminar con la aplicación literaria de las tesis sobre ingenios que definiera nuestro dilecto médico de resonancia mundial.Cervantes learned about Don Quixote's derangement through Juan Huarte de San Juan's famous treatise Examen de ingenios, where he deployed the humoral theory to explain human body and character. In this article Huarte's biography will be summarized, analyzing the academic background of golden age Spanish doctors, and the impact of Examen in Early Modern Europe and in Cervante's work by examining Don Quixote's melancholy and Licenciado Vidriera's madness. Finally, I will show how the internationally famous Spanish doctor's thesis was used in literature.

  5. Ladrillo and Tales of Juan Bobo: Puerto Rican Folk Tales.

    Science.gov (United States)

    Matos, Reinaldo; Matos, Ana

    These two illustrated elementary readers contain the Spanish and English versions of the Puerto Rican folk tales, "Ladrillo" and "Cuentos de Juan Bobo." They are part of a series of reading materials for elementary-level migrant children. These materials are intended to help the child relate to his culture, develop interest in knowing about it and…

  6. Ladrillo and Tales of Juan Bobo: Puerto Rican Folk Tales.

    Science.gov (United States)

    Matos, Reinaldo; Matos, Ana

    These two illustrated elementary readers contain the Spanish and English versions of the Puerto Rican folk tales, "Ladrillo" and "Cuentos de Juan Bobo." They are part of a series of reading materials for elementary-level migrant children. These materials are intended to help the child relate to his culture, develop interest in…

  7. El silencio como estrategia en la obra de Juan Rulfo

    OpenAIRE

    Gómez López-Quiñones, Loreto

    2013-01-01

    Juan Rulfo utiliza el silencio como un espacio para oír y decir mejor en el contexto del México postrevolucionario. Inspirándonos en la terminología de Spivak, pretendemos mostrar el silencio de Rulfo como una estrategia de resistencia ante la representación occidental del Otro.

  8. San Juan Basin, USA; coalbed methane development and production

    Energy Technology Data Exchange (ETDEWEB)

    Gluskoter, H. [US Geological Survey, Reston, VA (USA)

    2002-07-01

    Twenty one slides/overheads outline the talk on production of coalbed methane from sedimentary basins in the USA. Figures are given for production and reserves for the year 2000. The San Juan basin's geologic structure, containing resources of the Cretaceous coal age, primarily in the Fruitland Formation, is described. 4 refs.

  9. Offshore finfish mariculture in the Strait of Juan de Fuca

    Energy Technology Data Exchange (ETDEWEB)

    Rensel, Jack; Kiefer, Dale; Forster, John R.; Woodruff, Dana L.; Evans, Nathan R.

    2007-10-07

    Finfish mariculture has existed in the U.S. Pacific Northwest for over thirty years, but for the past 15 years most effort has focused on culture of Atlantic salmon in protected, inshore cage sites. The Strait of Juan de Fuca (the "Strait") is a large area with relatively sparce shoreline development and several apparent advantages for mariculture using offshore technology.

  10. Putting Educational Attainment First: An Interview with Juan Sepulveda

    Science.gov (United States)

    Carreon, Joe

    2011-01-01

    This article presents an interview with Juan Sepulveda. Sepulveda was appointed by U.S. Secretary of Education Arne Duncan to the position of Executive Director of the White House Initiative on Educational Excellence for Hispanics on May 19, 2009. In this capacity, Sepulveda is responsible for directing the efforts of the White House Initiative in…

  11. Ladrillo and Tales of Juan Bobo: Puerto Rican Folk Tales.

    Science.gov (United States)

    Matos, Reinaldo; Matos, Ana

    These two illustrated elementary readers contain the Spanish and English versions of the Puerto Rican folk tales, "Ladrillo" and "Cuentos de Juan Bobo." They are part of a series of reading materials for elementary-level migrant children. These materials are intended to help the child relate to his culture, develop interest in…

  12. O narrador solidário de Juan Coytisolo

    Directory of Open Access Journals (Sweden)

    Annete de Almeida Faria

    2011-11-01

    Full Text Available Resumo: Este artigo se propõe a analisar a construção do narrador de Cuaderno de Sarajevo: anotaciones de un viaje a la barbarie (1993, do escritor espanhol Juan Goytisolo, como expressão estética do seu compromisso ético. Tomamos como referência O narrador de Walter Benjamin para entender o narrador goytisolano como aquele que através das viagens busca o “outro”, elabora um arquivo memorial polifônico, misturando diferentes tipos de linguagem para dar voz às minorias.Palavras-chave: Literatura espanhola; Juan Goytisolo; Cuaderno de Sarajevo: anotaciones de un viaje a la barbarie; Walter Benjamin; O narrador; polifonia.Resumen: El propósito de este artículo consiste en analizar la construcción del narrador de Cuaderno de Sarajevo: anotaciones de un viaje a la barbarie (1993, del escritor español Juan Goytisolo, como expresión estética de su compromisso ético. Tomamos como base el ensayo El narrador, de Walter Benjamin, para comprender al narrador goytisolano como el que viaja en busca del “oro” (otredad para componer un archivo memorial polifónico, mezclando diferentes tipos de lenguaje para dar voz a las minorías.Palabras-clave: Literatura española; Juan Goytisolo; Cuaderno de Sarajevo: anotaciones de un viaje a la barbarie; Walter Benjamin; El narrador; polifonía.Keywords: Spanish literature; Juan Goytisolo; Cuaderno de Sarajevo: anotaciones de un viaje a la barbarie; Walter Benjamin; El narrador; poliphony.

  13. Surface oxide effect on optical sensing and photoelectric conversion of α-In2Se3 hexagonal microplates.

    Science.gov (United States)

    Ho, Ching-Hwa; Lin, Chien-Hao; Wang, Yi-Ping; Chen, Ying-Cen; Chen, Shin-Hong; Huang, Ying-Sheng

    2013-03-01

    The surface formation oxide assists of visible to ultraviolet photoelectric conversion in α-In2Se3 hexagonal microplates has been explored. Hexagonal α-In2Se3 microplates with the sizes of 10s to 100s of micrometers were synthesized and prepared by the chemical vapor transport method using ICl3 as a transport agent. Many vacancies and surface imperfection states have been found in the bulk and on the surface of the microplate because of the intrinsic defect nature of α-In2Se3. To discover physical and chemical properties and finding technological uses of α-In2Se3, several experiments including transmission electron miscopy (TEM), X-ray photoelectron spectroscopy (XPS), surface photovoltage (SPV), photoluminescence (PL), surface photoresponse (SPR), photoconductivity (PC), and thermoreflectance (TR) measurements have been carried out. Experimental results of TEM, XPS, SPV, PL, and SPR measurements show that a surface oxidation layer α-In2Se3-3xO3x (0 ≤ x ≤ 1) has formed on the crystal face of α-In2Se3 in environmental air with the inner layer content close to In2Se3 but the outermost layer content approaching In2O3. The near band edge transitions of α-In2Se3 microplates have been probed experimentally by TR and PC measurements. The direct band gap of α-In2Se3 has been determined to be 1.453 eV. The SPV result shows a maximum quantum efficiency of the surface oxide α-In2Se3-3xO3x (0 ≤ x ≤ 1) that presents a peak photoresponse near 2.18 eV. The analyses of SPV, SPR, PL, TR, and PC measurements revealed that the surface oxide layer facilitates the conversion of the ultraviolet to the visible range while the native defects (Se and In vacancies) sustain photoconductivity in the near-infrared region. On the basis of the experimental results a wide-energy-range photodetector that combines PC- and SPR-mode operations for α-In2Se3 microplate has been made. The testing results show a well-behaved function of photoelectric conversion in the near-infrared to

  14. PANEGÍRICO A JUAN ANTONIO SAMARANCH: Juan Antonio Samaranch Torelló: Una obra inabarcable

    Directory of Open Access Journals (Sweden)

    Alejandro Blanco Bravo

    2010-06-01

    Full Text Available Me gustaría abstraerme de mis sentimientos personales para poder describir en estas pocas líneas la figura del tristemente desaparecido Juan Antonio Samaranch Torrelló. Pero mi amistad y, sobre todo, mi admiración por él me lo impide. Necesitaría más espacio, más tiempo y más palabras para expresar mis sentimientos. Toda su vida la dedicó al deporte y a su gestión. Ha muerto con 89 años y lo hizo sin concederse un respiro, sin renunciar un instante a vivir el deporte desde todas sus aristas. Seguro que en sus últimos instantes pensó que la vida se le había quedado corta, que le faltó tiempo para seguir trabajando. Su obra es inabarcable. Fue un hombre de una inteligencia superior, que tuvo la paciencia y el deseo ferviente de llevar de la mano a todo un universo deportivo. El deporte era su pasión y el trabajo su devoción.

  15. Un contrato inédito de Juan de Juanes. El retablo de la Cofradía de la Sangre de Cristo de Valencia (1539

    Directory of Open Access Journals (Sweden)

    Gómez-Ferrer, Mercedes

    2012-03-01

    Full Text Available This article is based on an unpublished contract signed in 1539 by Vicente and Juan Macip for an altarpiece for the Valencian Brotherhood of the Blood of Christ. The agreement stipulates that Juan, later known as Juan de Juanes, had to paint the scenes. Thus the document reinforces the hypothesis that Juan Macip was the most important painter in the Macip workshop during the 1530’s. It also fills a documental void in between the completion of the Silversmith’s Guild altarpiece (1534-1539 and the contract for the Fuente la Higuera altarpiece (1548. This study also verifies the popularity of the iconography of the seven sheddings of the Blood of Christ and processions of disciplinants.

    Se presenta un contrato inédito suscrito por la Cofradía de la Sangre de Cristo de la ciudad de Valencia con Vicente y Juan Macip en 1539. En él se especifica que sea pintado por Juan, más conocido con posterioridad como Juan de Juanes. Se refuerza la hipótesis que permite concretar a Juan Macip como el pintor más importante del taller de los Macip durante la década de los 30, al tiempo que se llena un vacío documental entre la entrega del retablo del gremio de plateros (1534-1539 y la contratación del retablo de Fuente la Higuera (1548. Se constata igualmente la popularidad de la iconografía de los siete derramamientos de la Sangre de Cristo y la procesión de los disciplinantes.

  16. Juan Gabriel and audience interpretation. cultural impressions of effeminacy and sexuality in Mexico.

    Science.gov (United States)

    Sowards, S K

    2000-01-01

    Juan Gabriel's purported effeminacy and sexuality have made him a controversial subject in Mexico, but still loved by fans. Juan Gabriel, by trying to gain acceptance into Mexican society, has become part of a hybrid culture, between the feminine/masculine and homosexual/bisexual/heterosexual groups. This study focuses on interviews with 20 participants who discuss Juan Gabriel's popularity and sexuality. The findings of the study indicate that Juan Gabriel may be considered by his fans to be effeminate, and consequently homosexual. Even though homophobia is widespread in Mexico, Juan Gabriel's fans tend to ignore or exoticize his sexuality, thus affording his success. It is also possible that Juan Gabriel, consciously or not, uses his controversial sexuality as a way to generate popular interest.

  17. Entrevista a Juan José Aquerreta (Arte y Parte)

    OpenAIRE

    Latorre-Izquierdo, J. (Jorge)

    2009-01-01

    Esta entrevista de Jorge Latorre al artista Juan José Aquerreta fue publicada en Arte y Parte con ocasión de una exposición de obra reciente del artista que tuvo lugar en la galería Marlborough de Madrid (Orfila, 1) desde el 25 de marzo de 2009. Un año antes había visto la luz el catálogo de toda su obra, publicado por el Gobierno de Navarra, y que incluye textos de Francisco Calvo Serraller, Enrique Andrés Ruíz o el propio Aquerreta, además de una larga entrevista que le hizo Juan Manuel Bon...

  18. Il mestiere di tradurre 5: Juan Carlos Reche

    Directory of Open Access Journals (Sweden)

    Juan Carlos Reche

    2015-07-01

    Full Text Available En la última década el traductor y poeta Juan Carlos Reche (Córdoba, 1976 ha dado a conocer algunos de los textos poéticos más relevantes y complejos de la literatura italiana de los últimos tiempos. Galardonado en 2013 con el Premio Nazionale per la Traduzione concedido por el Ministerio Italiano de Cultura, es además autor de poemarios propios como El dolor y la velocidad (Ed. Renacimiento, Sevilla, 1999 o Carrera del fruto (Ed. Pre-Textos, Valencia, 2006. Su doble condicion de poeta y traductor, el riesgo de enfrentarse a autores como Maurizio Cucchi, Giorgio Caproni o Giovanni Raboni, y la reciente aparición de la revista Años diez, que dirije junto al poeta Abraham Gragera, nos ha llevado a plantearle algunas preguntas sobre su labor en ambos campos. Entrevista de Juan Pérez Andrés.

  19. Oral anatomy in the sixteenth century: Juan Valverde de Amusco.

    Science.gov (United States)

    López-Valverde, A; Gómez de Diego, R; De Vicente, J

    2013-08-01

    In 1554 Juan Valverde de Amusco, a Spanish anatomist, wrote the History of the composition of the human body, a complete anatomical treatise that took as its model the Vesalius school of thought (La fábrica of Vesalius). Considered one of the most complete anatomical treatises of the Renaissance and one of the most widely read books of the sixteenth century, it was translated into four languages in its day. The first chapter, devoted to bones, provides a meticulously detailed analysis of the bones of the facial structures and of the teeth, their supporting structures, vascularisation and innervation. Juan Valverde de Amusco even describes techniques for reducing mandibular luxations. Even with the imprecise observations typical of the time the treatise must be considered an exceptional document.

  20. San Juan implements one-man survey system

    Energy Technology Data Exchange (ETDEWEB)

    Andrae, S. (San Juan Coal Co., Waterflow, NM (United States))

    1994-07-01

    Describes the one-man survey system which has been implemented at the San Juan surface mine in northwestern New Mexico. The Geodimeter System 4000, produced by Geotronics of Sweden, consists of a tripod-mounted electronic total station and a range rod-mounted remote positioning unit (RPU). A radio link between the tripod-mounted total station and the RPU enables one person to control the instrument and collect data. At San Juan the system has been used to survey overburden removal and mining. Only in cases where the pits become very long, and control cannot be set in the pit, is a two-person crew used. The system is useful for surveys of compliance projects and lends itself well to regrading work. 3 photos.

  1. The Effect of Thermal Annealing on Charge Transport in Organolead Halide Perovskite Microplate Field-Effect Transistors.

    Science.gov (United States)

    Li, Dehui; Cheng, Hung-Chieh; Wang, Yiliu; Zhao, Zipeng; Wang, Gongming; Wu, Hao; He, Qiyuan; Huang, Yu; Duan, Xiangfeng

    2017-01-01

    Transformation of unipolar n-type semiconductor behavior to ambipolar and finally to unipolar p-type behavior in CH3 NH3 PbI3 microplate field-effect transistors by thermal annealing is reported. The photoluminescence spectra essentially maintain the same features before and after the thermal annealing process, demonstrating that the charge transport measurement provides a sensitive way to probe low-concentration defects in perovskite materials.

  2. Cellphone-based hand-held microplate reader for point-of-care ELISA testing (Conference Presentation)

    Science.gov (United States)

    Berg, Brandon; Cortazar, Bingen; Tseng, Derek; Ozkan, Haydar; Feng, Steve; Wei, Qingshan; Chan, Raymond Y.; Burbano, Jordi; Farooqui, Qamar; Lewinski, Michael; Di Carlo, Dino; Garner, Omai B.; Ozcan, Aydogan

    2016-03-01

    Enzyme-linked immunosorbent assay (ELISA) in a microplate format has been a gold standard first-line clinical test for diagnosis of various diseases including infectious diseases. However, this technology requires a relatively large and expensive multi-well scanning spectrophotometer to read and quantify the signal from each well, hindering its implementation in resource-limited-settings. Here, we demonstrate a cost-effective and handheld smartphone-based colorimetric microplate reader for rapid digitization and quantification of immunoserology-related ELISA tests in a conventional 96-well plate format at the point of care (POC). This device consists of a bundle of 96 optical fibers to collect the transmitted light from each well of the microplate and direct all the transmission signals from the wells onto the camera of the mobile-phone. Captured images are then transmitted to a remote server through a custom-designed app, and both quantitative and qualitative diagnostic results are returned back to the user within ~1 minute per 96-well plate by using a machine learning algorithm. We tested this mobile-phone based micro-plate reader in a clinical microbiology lab using FDA-approved mumps IgG, measles IgG, and herpes simplex virus IgG (HSV-1 and HSV-2) ELISA tests on 1138 remnant patient samples (roughly 50% training and 50% testing), and achieved an overall accuracy of ~99% or higher for each ELISA test. This handheld and cost-effective platform could be immediately useful for large-scale vaccination monitoring in low-infrastructure settings, and also for other high-throughput disease screening applications at POC.

  3. 33 CFR 110.74c - Bahia de San Juan, PR.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Bahia de San Juan, PR. 110.74c Section 110.74c Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas § 110.74c Bahia de San Juan, PR. The waters of San Antonio Channel, Bahia de San Juan, eastward...

  4. Revision of the genera Microplitis and Snellenius (Hymenoptera, Braconidae, Microgastrinae from Area de Conservacion Guanacaste, Costa Rica, with a key to all species previously described from Mesoamerica

    Directory of Open Access Journals (Sweden)

    Jose L. Fernandez-Triana

    2015-07-01

    Full Text Available The genera Microplitis and Snellenius (Hymenoptera: Braconidae, Microgastrinae from Area de Conservacion Guanacaste (ACG, Costa Rica, are revised. A total of 28 new species are described: 23 of Snellenius (the first record for Mesoamerica and five of Microplitis. A key is provided to all new species and five species of Microplitis previously described from Mesoamerica. In ACG, all Microplitis were reared exclusively from Sphingidae, while all Snellenius were reared from Noctuoidea (Noctuidae and Erebidae. All of the wasp species with known host records are unambiguously specialists, parasitizing one or a few related hosts. Biological information (wasp cocoon and caterpillar hosts in the Neotropical region seems to differ from similar data reported in previous works for the Oriental region -but more studies on the world fauna are needed. Although the distinction between these two genera has been controversial, we consider that the available evidence, although not conclusive, suggests that these two genera are best kept as separate (based on the presence of at least a partial epicnemial carina in Snellenius, which is absent in Microplitis. The following 28 species, all authored by Fernández-Triana & Whitfield, are described as species nova: Microplitis adrianguadamuzi, M. alexanderrojasi, M. francopupulini, M. hebertbakeri, M. jorgehernandezi, Snellenius billburgeri, S. bobdressleri, S. donstonei, S. felipechavarriai, S. gerardoherrerai, S. irenebakerae, S. isidrochaconi, S. johnkressi, S. jorgecampabadali, S. jorgegomezlauritoi, S. josesarukhani, S. kerrydresslerae, S. lucindamcdadeae, S. luisdiegogomezi, S. mariakuzminae, S. mariamartachavarriae, S. phildevriesi, S. quiricojimenezi, S. robertoespinozai, S. sandyknappae, S. velvaruddae, S. vickifunkae, S. warrenwagneri.

  5. Azorín y Don Juan (1922): vidas paralelas

    OpenAIRE

    Pérez-Bustamante Mourier, Ana Sofía

    1998-01-01

    Análisis de la novela "Don Juan" (1922), de José Martínez Ruiz (a) Azorín, dentro de la tradición del Tenorio arrepentido y redimido. En este caso la ejemplaridad cristiana se une a la perspectiva regeneracionista, y el tema del arrepentimiento se desarrolla a través de una estética impresionista y simbolista.

  6. Teaching and Communicating Astronomy at Rey Juan Carlos University

    Science.gov (United States)

    Hernán-Obispo, M.; Serrano, A.; Aguirre, J.; Martín, P. San

    We present our activities of popularization of Astronomy at Rey Juan Carlos University in Madrid, especially our 30-h workshop for people older than 55 (University for the Elderly) held since the academic year 2002/2003. Our course aims to introduce the basic topics on Astronomy to a group of motivated students who, in most cases, were not able to complete their education in their youth due to the historical environment of Spain in the middle of the twentieth century.

  7. Development, optimization and validation of a rapid colorimetric microplate bioassay for neomycin sulfate in pharmaceutical drug products.

    Science.gov (United States)

    Francisco, Fabiane Lacerda; Saviano, Alessandro Morais; Pinto, Terezinha de Jesus Andreoli; Lourenço, Felipe Rebello

    2014-08-01

    Microbiological assays have been used to evaluate antimicrobial activity since the discovery of the first antibiotics. Despite their limitations, microbiological assays are widely employed to determine antibiotic potency of pharmaceutical dosage forms, since they provide a measure of biological activity. The aim of this work is to develop, optimize and validate a rapid colorimetric microplate bioassay for the potency of neomycin in pharmaceutical drug products. Factorial and response surface methodologies were used in the development and optimization of the choice of microorganism, culture medium composition, amount of inoculum, triphenyltetrazolium chloride (TTC) concentration and neomycin concentration. The optimized bioassay method was validated by the assessment of linearity (range 3.0 to 5.0μg/mL, r=0.998 and 0.994 for standard and sample curves, respectively), precision (relative standard deviation (RSD) of 2.8% and 4.0 for repeatability and intermediate precision, respectively), accuracy (mean recovery=100.2%) and robustness. Statistical analysis showed equivalency between agar diffusion microbiological assay and rapid colorimetric microplate bioassay. In addition, microplate bioassay had advantages concerning the sensitivity of response, time of incubation, and amount of culture medium and solutions required.

  8. A new cadmium reduction device for the microplate determination of nitrate in water, soil, plant tissue, and physiological fluids.

    Science.gov (United States)

    Crutchfield, James D; Grove, John H

    2011-01-01

    A reusable catalytic reductor consisting of 96 copperized-cadmium pins attached to a microplate lid was developed to simultaneously reduce nitrate (NO3-) to nitrite (NO2-) in all wells of a standard microplate. The resulting NO2- is analyzed colorimetrically by the Griess reaction using a microplate reader. Nitrate data from groundwater samples analyzed using the new device correlated well with data obtained by ion chromatography (r2 = 0.9959). Soil and plant tissue samples previously analyzed for NO3- in an interlaboratory validation study sponsored by the Soil Science Society of America were also analyzed using the new technique. For the soil sample set, the data are shown to correlate well with the other methods used (r2 = 0.9976). Plant data correlated less well, especially for samples containing low concentrations of NO3-. Reasons for these discrepancies are discussed, and new techniques to increase the accuracy of the analysis are explored. In addition, a method is presented for analyzing NO3- in physiological fluids (blood serum and urine) after matrix modification with Somogyi's reagent. A protocol for statistical validation of data when analyzing samples with complex matrixes is also established. The simplicity, adaptability, and low cost of the device indicate its potential for widespread application.

  9. HISTORICAL ECOTOXICOLOGICALASSESSMENT OF SAN JUAN ECOSYSTEM, SANTIAGO DE CUBA, CUBA

    Directory of Open Access Journals (Sweden)

    Argota, George

    2013-07-01

    Full Text Available Currently, the environmental decision-making related to watershed preservation is oriented under the precepts of an historical environmental assessment. The objective of this research was to conduct a historical ecotoxicological assessment of the San Juan de Santiago de Cuba ecosystem. This was considered a systematic environmental monitoring which ran from 2010 to 2013, made q?uarterly. Physicochemical parameters such as electrical conductivity, total alkalinity, total hardness, pH, total solids, dissolved oxygen, biochemical oxygen demand and chemical oxygen demand were determined. All parameters of environmental risk prediction were performed using the GECOTOX program. The concentrations of Cu, Zn, Pb and Cd on three target organs (brain, gills and liver in the biomonitor adult Gambusia punctata (Poecilidae were analyzed. In this species, the effect of total protein and enzymatic activity of acetylcholinesterase as biomarkers were evaluated. In general, the parameters were outside the values set used by the Cuban quality standards. The GECOTOX program said that the waters of the San Juan ecosystem presented a high rate of risk. The highest concentrations of metals were determined in the gills, liver and brain, respectively. Biomarker values w? ere high, being higher for females. Finally, it was concluded that from one year to another all responses were ascending; thus, the San Juan ecosystem presented ecotoxicological effects in time.

  10. Antibacterial properties of cyclodextrin-antiseptics-complexes determined by microplate laser nephelometry and ATP bioluminescence assay.

    Science.gov (United States)

    Finger, Susanne; Wiegand, Cornelia; Buschmann, Hans-Jürgen; Hipler, Uta-Christina

    2013-08-16

    Cyclodextrins (CDs) are able to form inclusion complexes with other molecules, thereby, protecting these guest molecules from degradation, enhancing their biocompatibility or influencing their physiological distribution while retaining their activity. Here, antibacterial effects of CD-complexes with the antiseptics chlorhexidine diacetate (CHX), iodine (IOD) and polihexanide (PHMB) were determined using two different in vitro methods, microplate laser nephelometry and an ATP bioluminescence assay. Laser nephelometry is a direct method for monitoring and evaluating growth of micro-organisms by measurement of the turbidity of the solution. In contrast, the ATP bioluminescence assay determines specifically the amount of metabolic active bacterial cells. The antibacterial effects of CD-antiseptics-complexes were examined for Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis and the results of both methods were compared in respect of calculated means of half maximal inhibitory concentrations (IC50) and statistical evaluated Pearson's correlation coefficients (r). It could be demonstrated that both methods showed a high comparability although they differ in the parameters tested. This study revealed that CD-complexes with CHX and PHMB were most effective against E. coli and the tested staphylococci. While CD-IOD-complexes obtained high activity against K. pneumoniae, P. aeruginosa was distinctly more resistant compared to the other bacteria.

  11. A rapid turbidometric microplate bioassay for accurate quantification of lactic acid bacteria bacteriocins.

    Science.gov (United States)

    Turcotte, Carl; Lacroix, Christophe; Kheadr, Ehab; Grignon, Louis; Fliss, Ismaïl

    2004-02-01

    A 1 day turbidometric microplate bioassay (TMB) was developed for the rapid, accurate and precise quantification of lactic acid bacteria (LAB) bacteriocins (nisin Z and pediocin PA-1). Parameters such as the concentration of the indicator strains and the incubation time were optimized for each bacteriocin. A high correlation coefficient (r(2)=0.992+/-0.004) was obtained for the exponential regression in the nisin Z concentration range of 20-120 ng/ml with 1 x 10(7) CFU indicator strain (Pediococcus acidilactici UL5) and an incubation time of 3 h. Using these parameters, the detection limit was estimated at 80 ng/ml (3.2 IU/ml), compared to 300 ng/ml for the agar diffusion assay (ADA). High precision (<7%) and accuracy (10%) were obtained for all nisin Z concentrations tested. Similar results were obtained with pediocin PA-1 with r(2)=0.993+/-0.005, a precision (8.2%) and an accuracy lower than 15%.

  12. Enzyme activity assay of glycoprotein enzymes based on a boronate affinity molecularly imprinted 96-well microplate.

    Science.gov (United States)

    Bi, Xiaodong; Liu, Zhen

    2014-12-16

    Enzyme activity assay is an important method in clinical diagnostics. However, conventional enzyme activity assay suffers from apparent interference from the sample matrix. Herein, we present a new format of enzyme activity assay that can effectively eliminate the effects of the sample matrix. The key is a 96-well microplate modified with molecularly imprinted polymer (MIP) prepared according to a newly proposed method called boronate affinity-based oriented surface imprinting. Alkaline phosphatase (ALP), a glycoprotein enzyme that has been routinely used as an indicator for several diseases in clinical tests, was taken as a representative target enzyme. The prepared MIP exhibited strong affinity toward the template enzyme (with a dissociation constant of 10(-10) M) as well as superb tolerance for interference. Thus, the enzyme molecules in a complicated sample matrix could be specifically captured and cleaned up for enzyme activity assay, which eliminated the interference from the sample matrix. On the other hand, because the boronate affinity MIP could well retain the enzymatic activity of glycoprotein enzymes, the enzyme captured by the MIP was directly used for activity assay. Thus, additional assay time and possible enzyme or activity loss due to an enzyme release step required by other methods were avoided. Assay of ALP in human serum was successfully demonstrated, suggesting a promising prospect of the proposed method in real-world applications.

  13. PCR verification of microplate phenotypic system identification for LAB from traditional Western Balkan raw milk cheeses

    Directory of Open Access Journals (Sweden)

    Diana Paveljšek

    2014-11-01

    Full Text Available Fermentation and ripening specificity of traditional cheeses are predominantly directed by the natural microbial community present in milk selected by the cheese-making environment and technology. Therefore the traditional cheeses are unique products with specific microbiota biodiversity. There are several approaches for the identification of microbial population, however all of them have certain advantages and disadvantages. In this study the eligibility and performance of the Biolog phenotypic identification system (Biolog, Inc. with GEN III microplates was tested. Parallel to this method, polymerase chain reaction with genus- and species-specific primers was performed. One hundred sixty-five isolates from nine types of artisan cheeses were isolated and analysed. Cheeses were produced from raw ewe’s milk in Slovenia, Bosnia and Herzegovina, Croatia and Serbia. The Biolog phenotypic identification system identified 90 isolates, but only 55 identifications acquired by the Biolog system were supported by polymerase chain reaction at a genus level and 28 at a species level. The obtained results showed that the reliability of commercial phenotypic identification systems was inadequate when analysing lactic acid bacteria isolates from natural, spontaneous fermentations and needs to be additionally corroborated by genotypic identification methods.

  14. Evidence for Moho-lower crustal transition depth diking and rifting of the Sierra Nevada microplate

    Science.gov (United States)

    Smith, Kenneth D.; Kent, Graham M.; Seggern, David P.; Driscoll, Neal W.; Eisses, Amy

    2016-10-01

    Lithospheric rifting most often initiates in continental extensional settings where "breaking of a plate" may or may not progress to sea floor spreading. Generally, the strength of the lithosphere is greater than the tectonic forces required for rupture (i.e., the "tectonic force paradox"), and it has been proposed that rifting requires basaltic magmatism (e.g., dike emplacement) to reduce the strength and cause failure, except for the case of a thin lithosphere (structure, each striking N45°W and dipping 50°NE. A single event at 30 km depth that locates on the implied dipping feature between the two swarms is further evidence for a single Moho-transition depth structure. We propose that basaltic or fluid emplacement at or near Moho depths weakens the upper mantle lid, facilitating lithospheric rupture of the Sierra Microplate. Similar to the LT sequence, the SV event is also associated with increased upper crustal seismicity. An 27 October 2011, Mw 4.7 earthquake occurred directly above the deep SV sequence at the base of the upper crustal seismogenic zone ( 15 km depth).

  15. High-Throughput Microplate-Based Assay to Monitor Plasma Membrane Wounding and Repair

    Directory of Open Access Journals (Sweden)

    Sarika Pathak-Sharma

    2017-07-01

    Full Text Available The plasma membrane of mammalian cells is susceptible to disruption by mechanical and biochemical damages that frequently occur within tissues. Therefore, efficient and rapid repair of the plasma membrane is essential for maintaining cellular homeostasis and survival. Excessive damage of the plasma membrane and defects in its repair are associated with pathological conditions such as infections, muscular dystrophy, heart failure, diabetes, and lung and neurodegenerative diseases. The molecular events that remodel the plasma membrane during its repair remain poorly understood. In the present work, we report the development of a quantitative high-throughput assay that monitors the efficiency of the plasma membrane repair in real time using a sensitive microplate reader. In this assay, the plasma membrane of living cells is perforated by the bacterial pore-forming toxin listeriolysin O and the integrity and recovery of the membrane are monitored at 37°C by measuring the fluorescence intensity of the membrane impermeant dye propidium iodide. We demonstrate that listeriolysin O causes dose-dependent plasma membrane wounding and activation of the cell repair machinery. This assay was successfully applied to cell types from different origins including epithelial and muscle cells. In conclusion, this high-throughput assay provides a novel opportunity for the discovery of membrane repair effectors and the development of new therapeutic compounds that could target membrane repair in various pathological processes, from degenerative to infectious diseases.

  16. Microplate assay for screening the antibacterial activity of Schiff bases derived from substituted benzopyran-4-one.

    Science.gov (United States)

    Amin, Rehab M; Abdel-Kader, Nora S; El-Ansary, Aida L

    2012-09-01

    Schiff bases (SB(1)-SB(3)) were synthesized from the condensation of 6-formyl-7-hydroxy-5-methoxy-2-methylbenzopyran-4-one with 2-aminopyridine (SB(1)), p-phenylenediamine (SB(2)) and o-phenylenediamine (SB(3)), while Schiff bases (SB(4)-SB(6)) were synthesized by condensation of 5,7-dihydroxy-6-formyl-2-methylbenzopyran-4-one with 2-aminopyridine (SB(4)), p-phenylenediamine (SB(5)) and o-phenylenediamine (SB(6)). Schiff bases were characterized using elemental analysis, IR, UV-Vis, (1)H NMR, (13)C NMR and mass spectroscopy. These compounds were screened for antibacterial activities by micro-plate assay technique. Escherichia coli and Staphylococcus capitis were exposed to different concentrations of the Schiff bases. Results showed that the antibacterial effect of these Schiff bases on Gram-negative bacteria were higher than that on Gram-positive bacteria moreover, the Schiff bases containing substituent OCH(3) on position five have higher antibacterial activity than that containing hydroxy group on the same position.

  17. Development and evaluation of a fluorescence microplate assay for quantification of heparins and other sulfated carbohydrates.

    Science.gov (United States)

    Lühn, Susanne; Schrader, Thomas; Sun, Wei; Alban, Susanne

    2010-05-01

    Due to their complex composition, quantification of heparins is difficult. On the one hand there are many biological tests, which only indirectly detect effects of the antithrombin-binding material. On the other hand direct quantitative methods are available but they are often insensitive, challenging, time-consuming or expensive. The aim of this study was to develop a sensitive, rapid, simple as well as inexpensive direct quantification assay suitable for routine analysis. Based on Polymer-H, a novel heparin complexing, fluorescent labeled synthetic polymer (lambda((ex)) 320nm, lambda((em)) 510nm), a microplate assay was developed and optimized. The specificity of the assay was evaluated by structure-assay response relationships studies using structurally defined glucan sulfates, heparins, and other natural and synthetic sulfated carbohydrates. The fluorescence intensity of Polymer-H (7.5microg/ml) showed to be concentration-dependently amplified by heparins as well as by other sulfated carbohydrates. The best sensitivity, accuracy and linearity were observed in a range from 0.63 to 5.0microg/ml heparins. No differences in the fluorescence between various heparins were observed, so that only one calibration curve is needed. In addition, all types of carbohydrates with a degree of sulfation (DS)> approximately 1.2 and a M(r)>3000 can be quantified as well. By own calibration curves also other sulfated carbohydrates like fondaparinux or other glycosaminoglycans (DS>0.4) can be determined.

  18. Rapid quantitative analysis of lipids using a colorimetric method in a microplate format.

    Science.gov (United States)

    Cheng, Yu-Shen; Zheng, Yi; VanderGheynst, Jean S

    2011-01-01

    A colorimetric sulfo-phospho-vanillin (SPV) method was developed for high throughput analysis of total lipids. The developed method uses a reaction mixture that is maintained in a 96-well microplate throughout the entire assay. The new assay provides the following advantages over other methods of lipid measurement: (1) background absorbance can be easily corrected for each well, (2) there is less risk of handling and transferring sulfuric acid contained in reaction mixtures, (3) color develops more consistently providing more accurate measurement of absorbance, and (4) the assay can be used for quantitative measurement of lipids extracted from a wide variety of sources. Unlike other spectrophotometric approaches that use fluorescent dyes, the optimal spectra and reaction conditions for the developed assay do not vary with the sample source. The developed method was used to measure lipids in extracts from four strains of microalgae. No significant difference was found in lipid determination when lipid content was measured using the new method and compared to results obtained using a macro-gravimetric method.

  19. Kinetic microplate bioassays for relative potency of antibiotics improved by partial Least Square (PLS) regression.

    Science.gov (United States)

    Francisco, Fabiane Lacerda; Saviano, Alessandro Morais; Almeida, Túlia de Souza Botelho; Lourenço, Felipe Rebello

    2016-05-01

    Microbiological assays are widely used to estimate the relative potencies of antibiotics in order to guarantee the efficacy, safety, and quality of drug products. Despite of the advantages of turbidimetric bioassays when compared to other methods, it has limitations concerning the linearity and range of the dose-response curve determination. Here, we proposed to use partial least squares (PLS) regression to solve these limitations and to improve the prediction of relative potencies of antibiotics. Kinetic-reading microplate turbidimetric bioassays for apramacyin and vancomycin were performed using Escherichia coli (ATCC 8739) and Bacillus subtilis (ATCC 6633), respectively. Microbial growths were measured as absorbance up to 180 and 300min for apramycin and vancomycin turbidimetric bioassays, respectively. Conventional dose-response curves (absorbances or area under the microbial growth curve vs. log of antibiotic concentration) showed significant regression, however there were significant deviation of linearity. Thus, they could not be used for relative potency estimations. PLS regression allowed us to construct a predictive model for estimating the relative potencies of apramycin and vancomycin without over-fitting and it improved the linear range of turbidimetric bioassay. In addition, PLS regression provided predictions of relative potencies equivalent to those obtained from agar diffusion official methods. Therefore, we conclude that PLS regression may be used to estimate the relative potencies of antibiotics with significant advantages when compared to conventional dose-response curve determination.

  20. Investigation of mitochondrial dysfunction by sequential microplate-based respiration measurements from intact and permeabilized neurons.

    Directory of Open Access Journals (Sweden)

    Pascaline Clerc

    Full Text Available Mitochondrial dysfunction is a component of many neurodegenerative conditions. Measurement of oxygen consumption from intact neurons enables evaluation of mitochondrial bioenergetics under conditions that are more physiologically realistic compared to isolated mitochondria. However, mechanistic analysis of mitochondrial function in cells is complicated by changing energy demands and lack of substrate control. Here we describe a technique for sequentially measuring respiration from intact and saponin-permeabilized cortical neurons on single microplates. This technique allows control of substrates to individual electron transport chain complexes following permeabilization, as well as side-by-side comparisons to intact cells. To illustrate the utility of the technique, we demonstrate that inhibition of respiration by the drug KB-R7943 in intact neurons is relieved by delivery of the complex II substrate succinate, but not by complex I substrates, via acute saponin permeabilization. In contrast, methyl succinate, a putative cell permeable complex II substrate, failed to rescue respiration in intact neurons and was a poor complex II substrate in permeabilized cells. Sequential measurements of intact and permeabilized cell respiration should be particularly useful for evaluating indirect mitochondrial toxicity due to drugs or cellular signaling events which cannot be readily studied using isolated mitochondria.

  1. Investigation of mitochondrial dysfunction by sequential microplate-based respiration measurements from intact and permeabilized neurons.

    Science.gov (United States)

    Clerc, Pascaline; Polster, Brian M

    2012-01-01

    Mitochondrial dysfunction is a component of many neurodegenerative conditions. Measurement of oxygen consumption from intact neurons enables evaluation of mitochondrial bioenergetics under conditions that are more physiologically realistic compared to isolated mitochondria. However, mechanistic analysis of mitochondrial function in cells is complicated by changing energy demands and lack of substrate control. Here we describe a technique for sequentially measuring respiration from intact and saponin-permeabilized cortical neurons on single microplates. This technique allows control of substrates to individual electron transport chain complexes following permeabilization, as well as side-by-side comparisons to intact cells. To illustrate the utility of the technique, we demonstrate that inhibition of respiration by the drug KB-R7943 in intact neurons is relieved by delivery of the complex II substrate succinate, but not by complex I substrates, via acute saponin permeabilization. In contrast, methyl succinate, a putative cell permeable complex II substrate, failed to rescue respiration in intact neurons and was a poor complex II substrate in permeabilized cells. Sequential measurements of intact and permeabilized cell respiration should be particularly useful for evaluating indirect mitochondrial toxicity due to drugs or cellular signaling events which cannot be readily studied using isolated mitochondria.

  2. Geosmin induces genomic instability in the mammalian cell microplate-based comet assay.

    Science.gov (United States)

    Silva, Aline Flor; Lehmann, Mauricio; Dihl, Rafael Rodrigues

    2015-11-01

    Geosmin (GEO) (trans-1,10-dimethyl-trans-9-decalol) is a metabolite that renders earthy and musty taste and odor to water. Data of GEO genotoxicity on mammalian cells are scarce in the literature. Thus, the present study assessed the genotoxicity of GEO on Chinese hamster ovary (CHO) cells in the microplate-based comet assay. The percent of tail DNA (tail intensity (TI)), tail moment (TM), and tail length (TL) were used as parameters for DNA damage assessment. The results demonstrated that concentrations of GEO of 30 and 60 μg/mL were genotoxic to CHO cells after 4- and 24-h exposure periods, in all parameters evaluated, such as TI, TM, and TL. Additionally, GEO 15 μg/mL was genotoxic in the three parameters only in the 24-h exposure time. The same was observed for GEO 7.5 μg/mL, which induced significant DNA damage observed as TI in the 24-h treatment. The results present evidence that exposure to GEO may be associated with genomic instability in mammalian cells.

  3. The Suckling Hills Fault, Kayak Island Zone, and accretion of the Yakutat microplate, Alaska

    Science.gov (United States)

    Chapman, James B.; Worthington, Lindsay L.; Pavlis, Terry L.; Bruhn, Ronald L.; Gulick, Sean P.

    2011-12-01

    The Suckling Hills and Kayak Island are isolated mountain blocks located along strike from each other within the foreland of the St. Elias orogen in southern Alaska. These blocks preserve an erosional surface that was deformed by slip on northwest-dipping reverse faults in the Pleistocene. We suggest that the Suckling Hills Fault and Kayak Island Zone form a segmented fault network that links with the Bering Glacier structure to the north. This fault network separates the central Yakataga fold and thrust belt from complex, multiply deformed structures in the western syntaxis. Ongoing accretion of the Yakutat microplate to North America results in translation of structures of the fold and thrust belt into the western syntaxis. The composite Suckling Hills Fault, Kayak Island Zone, and Bering Glacier structure may have formed because the older structures of the fold and thrust belt were unfavorably oriented within the western syntaxis region. This pattern of deformation provides a template for understanding the complex deformation within the core of the western syntaxis and predicts refolding and straightening of the western syntaxis margin with continued accretion. This study provides an analog for structural overprinting and changing deformation patterns through time in orogenic corners.

  4. Microplate array diagonal gel electrophoresis for cohort studies of microsatellite loci.

    Science.gov (United States)

    Chen, Xiao-he; O'Dell, Sandra D; Day, Ian N M

    2002-05-01

    After PCR amplification, we have achieved precise sizing of trinucleotide and tetranucleotide microsatellite alleles on 96-well open-faced polyacrylamide microplate array diagonal gel electrophoresis (MADGE) gels: two tetranucleotide repeats, HUMTHOI (five alleles 248-263 bp) and DYS390 (eight alleles 200-228 bp), and DYS392, a trinucleotide repeat (eight alleles 210-231 bp). A gel matrix of Duracryl, a high mechanical strength polyacrylamide derivative, and appropriate ionic conditions provide the 1.3%-1.5% band resolution required. No end-labeling of primers is needed, as the sensitive Vistra Green intercalating dye is used for the visualization of bands. Co-run markers bracketing the PCR fragments ensure accurate sizing without inter-lane variability. Electrophoresis of multiple gels in a thermostatically controlled tank allows up to 1000 samples to be run in 90 min. Gel images were analyzed using a Fluorlmager 595 fluorescent scanning system, and alleles were identified using Phoretix software for band migration measurement and Microsoft Excel to compute fragment sizes. Estimated sizes were interpolated precisely to achieve accurate binning. Microsatellite-MADGE represents a utilitarian methodfor high-throughput genotyping in cohort studies, using standard laboratory equipment.

  5. Microplate assay for quantitation of neutral lipids in extracts from microalgae.

    Science.gov (United States)

    Higgins, Brendan T; Thornton-Dunwoody, Alexander; Labavitch, John M; VanderGheynst, Jean S

    2014-11-15

    Lipid quantitation is widespread in the algae literature, but popular methods such as gravimetry, gas chromatography and mass spectrometry (GC-MS), and Nile red cell staining suffer drawbacks, including poor quantitation of neutral lipids, expensive equipment, and variable results among algae species, respectively. A high-throughput microplate assay was developed that uses Nile red dye to quantify neutral lipids that have been extracted from algae cells. Because the algal extracts contained pigments that quenched Nile red fluorescence, a mild bleach solution was used to destroy pigments, resulting in a nearly linear response for lipid quantities in the range of 0.75 to 40 μg. Corn oil was used as a standard for quantitation, although other vegetable oils displayed a similar response. The assay was tested on lipids extracted from three species of Chlorella and resulted in close agreement with triacylglycerol (TAG) levels determined by thin layer chromatography. The assay was found to more accurately measure algal lipids conducive to biodiesel production and nutrition applications than the widely used gravimetric assay. Assay response was also consistent among different species, in contrast to Nile red cell staining procedures. Copyright © 2014 Elsevier Inc. All rights reserved.

  6. Antimycobacterial screening of traditional medicinal plants using the microplate resazurin assay.

    Science.gov (United States)

    Webster, Duncan; Lee, Timothy D G; Moore, Jill; Manning, Tracy; Kunimoto, Dennis; LeBlanc, Darren; Johnson, John A; Gray, Christopher A

    2010-06-01

    Multidrug-resistant Mycobacterium tuberculosis strains have rapidly become a global health concern. North American First Nations communities have used traditional medicines for generations to treat many pulmonary infections. In this study, we evaluated the antimycobacterial activity of 5 medicinal plants traditionally used as general therapeutics for pulmonary illnesses and specifically as treatments for tuberculosis. Aqueous extracts of Aralia nudicaulis, Symplocarpus foetidus, Heracleum maximum, Juniperus communis, and Acorus calamus were screened for antimycobacterial activity against Bacillus Calmette-Guérin, Mycobacterium avium, and M. tuberculosis H37Ra using the colorimetric microplate resazurin assay. Extracts of Acorus calamus and H. maximum root demonstrated significant antimycobacterial activity comparable to that of the rifampin control (2 microg/mL). Evaluation of the cytotoxicity of these 2 extracts using the MTT assay also showed that the extracts were less toxic to 3 human cell lines than was the DMSO positive control. This study demonstrates that aqueous extracts of the roots of H. maximum and Acorus calamus possess strong in vitro antimycobacterial activity, validates traditional knowledge, and provides potential for the development of urgently needed novel antituberculous therapeutics.

  7. La encrucijada en Pedro Páramo de Juan Rulfo / The Crossroads in Pedro Páramo by Juan Rulfo

    Directory of Open Access Journals (Sweden)

    Lilia Leticia García-Peña

    2015-08-01

    Full Text Available La obra de Juan Rulfo sintetiza la realidad del campo mexicano, captura el contorno de las identidades individuales y de la estructura de la interacción social del mundo que tanto le preocupó y del que es intérprete indiscutible, pero también supo convertir en materia narrativa aquellas atmósferas que rebasan el contexto mexicano y social. En este trabajo abordaré el tema de la encrucijada en el imaginario simbólico de Pedro Páramo (1955 como expresión de una reelaboración del mito de Hermes, analizaré cómo la novela de Juan Rulfo muestra estos ecos herméticos y qué sentido estético y sociocultural tiene esta resonancia mítica.

  8. Aprendizajes del Accidente de San Juan Ixhuatepec-México Learning from the Accident in San Juan Ixhuatepec-Mexico

    Directory of Open Access Journals (Sweden)

    Antioco López-Molina

    2012-01-01

    Full Text Available El índice Dow de fuego y explosión y la metodología del análisis cuantitativo del riesgo son aplicados para analizar uno de los accidentes más desafortunados en la historia mexicana: la explosión de tanques de almacenamiento de gas en la planta de San Juan Ixhuatepec en México. Estimaciones adicionales de la sobrepresión y radiación térmica producida durante el siniestro son llevadas a cabo para explicar el efecto dominó producido como consecuencia del incidente. Estas estimaciones dan evidencia de que las instalaciones en la planta de San Juan Ixhuatepec tenían un nivel de riesgo moderado lo cual se contrapone a la percepción de la sociedad en relación al riesgo de la compañía Pemex, propietaria de la planta. Este trabajo concluye que el accidente fue principalmente debido a errores humanos, así como a la pésima ubicación de la planta.The Dow fire and explosion index and the quantitative risk assessment methodology are applied to analyze one of the most unfortunate accidents in Mexican history: the explosion of gas storage tanks at the San Juan Ixhuatepec plant in Mexico. Additional calculations of over-pressure and thermal radiation are carried out to further explain the domino effect produced as a consequence of the accident. The results give evidence that facilities in San Juan Ixhuatepec had a moderate risk level which is in disagreement with people’s perception of the Pemex company, proprietary of the plant. This study concludes that the accident was mainly due to human error, as well as the inappropriate location of the plant.

  9. La construcción de un mito : fortuna crítica de Juan de Juanes en los siglos XVI y XVII

    Directory of Open Access Journals (Sweden)

    Miguel Falomir Faus

    1999-01-01

    Full Text Available Este artículo pretende analizar los inicios de la fortuna crítica de Juan de Juanes en los siglos XVI y XVII. La «leyenda» de Juanes se asentó en su triple condición de gloria local, pintor ejemplar y artista piadoso, y quienes la forjaron, de Escolano a Vicente Vitoria pasando por Pacheco o Jusepe Martínez, no dudaron en potenciar, e incluso distorsionar, aquellas facetas de la personalidad de Juanes afines a sus intereses particulares.This article seeks to analyze the origins of the historiographical treatment of Juan de Juanes in the sixteenth and seventeenth centuries. The «myth» of Juanes was posited on three aspects: as exemplary and illustrious citizen, model forpainters, and pious artist. Those who forged this image of the artist, from Escolano to Vicente Vitoria and passing by Pacheco or Jusepe Martínez, did not doubt in emphasizing and even distorting those aspects of his personality and life that affirmed their individual interests.

  10. Hydrologic data from wells at or in the vicinity of the San Juan coal mine, San Juan County, New Mexico

    Science.gov (United States)

    Stewart, Anne M.; Thomas, Nicole

    2015-01-01

    In 2010, in cooperation with the Mining and Minerals Division (MMD) of the State of New Mexico Energy, Minerals and Natural Resources Department, the U.S. Geological Survey (USGS) initiated a 4-year assessment of hydrologic conditions at the San Juan coal mine (SJCM), located about 14 miles west-northwest of the city of Farmington, San Juan County, New Mexico. The mine produces coal for power generation at the adjacent San Juan Generating Station (SJGS) and stores coal-combustion byproducts from the SJGS in mined-out surface-mining pits. The purpose of the hydrologic assessment is to identify groundwater flow paths away from SJCM coal-combustion-byproduct storage sites that might allow metals that may be leached from coal-combustion byproducts to eventually reach wells or streams after regional dewatering ceases and groundwater recovers to predevelopment levels. The hydrologic assessment, undertaken between 2010 and 2013, included compilation of existing data. The purpose of this report is to present data that were acquired and compiled by the USGS for the SJCM hydrologic assessment.

  11. Long-term GNSS measurements from the northern Adria microplate reveal fault-induced fluid mobilization

    Science.gov (United States)

    Rossi, Giuliana; Zuliani, David; Fabris, Paolo

    2016-10-01

    Due to the development of denser permanent Global Navigation Satellite System (GNSS) networks over the last decade, the observation of transient deformations has significantly increased, mainly in high strain-rate zones. We analyzed the data from a group of permanent GNSS sites on the N-Adria microplate, where anomalous southward tilting and low-frequency tremors preceded the 1976 Mw = 6.4 earthquake. We present records from different stations of a transient signal with an approximately 2-year period that propagated through the northern edge of Adria, in a region 150 km wide. This represents the first time a transient deformation event has been observed in a continental collision area. We exclude surface and groundwater hydrological load effects because we corrected the data for such effects at seasonal, annual, and multiyear scales. The movement is initially upward, except in one location, with slight tilting parallel to the direction of the main tectonic structures. Later, the opposite behavior is observed. The novel methods used include earthquake location techniques and tomographic inversion of the arrival times. These methods demonstrated that the transient source was located spatially and temporally close to the 2004 Mw = 5.2 event in Bovec (Slovenia), attributed to the activity of the Ravne fault. We interpret the transient rises as the expression of a porosity wave, possibly produced by fault valve behavior of the NW tip of the Ravne fault. The propagation velocity is consistent with this hypothesis. As a further test, we invert the arrival times of the transient through hydraulic tomography to obtain hydraulic diffusivity: the values are compatible with the lithotypes present in the region and the literature. By substituting the tomographic velocity and diffusivity in the solitary/porosity wave equation, we infer an initial effective stress of approximately 0.23 bar, sufficient to alter the equilibrium of some fault segments and influence the subsequent

  12. Electrophysiological and behavioral responses of Microplitis mediator (Hymenoptera: Braconidae) to caterpillar-induced volatiles from cotton.

    Science.gov (United States)

    Yu, Huilin; Zhang, Yongjun; Wyckhuys, Kris A G; Wu, Kongming; Gao, Xiwu; Guo, Yuyuan

    2010-04-01

    Microplitis mediator Haliday (Hymenoptera: Braconidae) is an important larval endoparasitoid of various lepidopteran pests, including Helicoverpa armigera (Hübner). In China, H. armigera is a key pest of cotton and is currently the focus of several biological control efforts that use M. mediator as principal natural enemy of this pest. To improve the success of biological control efforts, behavioral studies are needed that shed light on the interaction between M. mediator and H. armigera. In this study, we determined M. mediator response to volatile compounds from undamaged, mechanically injured, or H. armigera--damaged plants and identified attractive volatiles. In Y-tube olfactometer assays, we found that mechanically damaged plants and/or plants treated with H. armigera oral secretions did not attract wasps. However, volatiles from H. armigera-damaged plants elicited a strong attraction of both M. mediator sexes. Headspace extracts from H. armigera-damaged cotton were analyzed by coupled gas chromatography-electroantennographic detection (GC-EAD), and a total of seven different compounds were found to elicit electroantennogram (EAG) responses, including an unknown compound. Six different EAD-active volatiles were identified from caterpillar-damaged cotton plants, of which 3, 7-dimethyl-1, 3, 6-octatriene and (Z)-3-hexenyl acetate were the principal compounds. Olfactometer assays indicated that individual synthetic compounds of 3, 7-dimethyl-1, 3, 6-octatriene, (Z)-3-hexenyl acetate, and nonanal were attractive to M. mediator. Field cage studies showed that parasitism of H. armigera larvae by M. mediator was higher on cotton plants to which 3,7-dimethyl-1,3, 6-octatriene was applied. Our results show that the combination of terpenoids and green leaf volatiles may not only facilitate host, mate, or food location but may also increase H. armigera parasitism by M. mediator.

  13. Automated panning and screening procedure on microplates for antibody generation from phage display libraries.

    Science.gov (United States)

    Turunen, Laura; Takkinen, Kristiina; Söderlund, Hans; Pulli, Timo

    2009-03-01

    Antibody phage display technology is well established and widely used for selecting specific antibodies against desired targets. Using conventional manual methods, it is laborious to perform multiple selections with different antigens simultaneously. Furthermore, manual screening of the positive clones requires much effort. The authors describe optimized and automated procedures of these processes using a magnetic bead processor for the selection and a robotic station for the screening step. Both steps are performed in a 96-well microplate format. In addition, adopting the antibody phage display technology to automated platform polyethylene glycol precipitation of the enriched phage pool was unnecessary. For screening, an enzyme-linked immunosorbent assay protocol suitable for a robotic station was developed. This system was set up using human gamma-globulin as a model antigen to select antibodies from a VTT naive human single-chain antibody (scFv) library. In total, 161 gamma-globulin-selected clones were screened, and according to fingerprinting analysis, 9 of the 13 analyzed clones were different. The system was further tested using testosterone bovine serum albumin (BSA) and beta-estradiol-BSA as antigens with the same library. In total, 1536 clones were screened from 4 rounds of selection with both antigens, and 29 different testosterone-BSA and 23 beta-estradiol-BSA binding clones were found and verified by sequencing. This automated antibody phage display procedure increases the throughput of generating wide panels of target-binding antibody candidates and allows the selection and screening of antibodies against several different targets in parallel with high efficiency.

  14. Transdermal film-loaded finasteride microplates to enhance drug skin permeation: Two-step optimization study.

    Science.gov (United States)

    Ahmed, Tarek A; El-Say, Khalid M

    2016-06-10

    The goal was to develop an optimized transdermal finasteride (FNS) film loaded with drug microplates (MIC), utilizing two-step optimization, to decrease the dosing schedule and inconsistency in gastrointestinal absorption. First; 3-level factorial design was implemented to prepare optimized FNS-MIC of minimum particle size. Second; Box-Behnken design matrix was used to develop optimized transdermal FNS-MIC film. Interaction among MIC components was studied using physicochemical characterization tools. Film components namely; hydroxypropyl methyl cellulose (X1), dimethyl sulfoxide (X2) and propylene glycol (X3) were optimized for their effects on the film thickness (Y1) and elongation percent (Y2), and for FNS steady state flux (Y3), permeability coefficient (Y4), and diffusion coefficient (Y5) following ex-vivo permeation through the rat skin. Morphological study of the optimized MIC and transdermal film was also investigated. Results revealed that stabilizer concentration and anti-solvent percent were significantly affecting MIC formulation. Optimized FNS-MIC of particle size 0.93μm was successfully prepared in which there was no interaction observed among their components. An enhancement in the aqueous solubility of FNS-MIC by more than 23% was achieved. All the studied variables, most of their interaction and quadratic effects were significantly affecting the studied variables (Y1-Y5). Morphological observation illustrated non-spherical, short rods, flakes like small plates that were homogeneously distributed in the optimized transdermal film. Ex-vivo study showed enhanced FNS permeation from film loaded MIC when compared to that contains pure drug. So, MIC is a successful technique to enhance aqueous solubility and skin permeation of poor water soluble drug especially when loaded into transdermal films.

  15. Don Juan, de Gonzalo Torrente Ballester: el monstruo en su laberinto

    OpenAIRE

    1995-01-01

    Estudio de la novela "Don Juan" (1963), de Gonzalo Torrente Ballester, dentro de la tradición del mito de Don Juan y como relato de técnica impecable que consigue el reto de hacer una novela fantástica larga.

  16. 77 FR 61632 - Final Environmental Impact Statement for Cattle Point Road Relocation, San Juan Island National...

    Science.gov (United States)

    2012-10-10

    ... National Park Service Final Environmental Impact Statement for Cattle Point Road Relocation, San Juan.... ACTION: Notice of Availability of the Final Environmental Impact Statement for Cattle Point Road... bluff erosion that threatens a segment of the Cattle Point Road located in San Juan Island...

  17. CHARACTERIZING THE ORGANIC MATTER IN SURFACE SEDIMENTS FROM THE SAN JUAN BAY ESTUARY

    Science.gov (United States)

    The San Juan Bay Estuary (SJBE) is located on the north coast of Puerto Rico and includes the San Juan Bay, San José Lagoon, La Torrecilla Lagoon and Piñones Lagoon, as well as the Martín Peña and the Suárez Canals. The SJBE watershed has the highest...

  18. 33 CFR 110.240 - San Juan Harbor, P.R.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false San Juan Harbor, P.R. 110.240 Section 110.240 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Anchorage Grounds § 110.240 San Juan Harbor, P.R. (a) The anchorage...

  19. CHARACTERIZING THE ORGANIC MATTER IN SURFACE SEDIMENTS FROM THE SAN JUAN BAY ESTUARY.

    Science.gov (United States)

    The San Juan Bay Estuary (SJBE) is located on the north coast of Puerto Rico and includes the San Juan Bay, San José Lagoon, La Torrecilla Lagoon and Piñones Lagoon, as well as the Martín Peña and the Suárez Canals. The SJBE watershed has the highest...

  20. Eficiência dos testes COPAN (Microplate e Single na detecção de resíduos de antimicrobianos no leite Efficacy of COPAN (Microplate and Single kits in detection of antimicrobials residues in milk

    Directory of Open Access Journals (Sweden)

    C.G.M.S.C. Tenório

    2009-04-01

    Full Text Available Avaliou-se a eficiência dos testes microbiológicos COPAN (Microplate e Single para detecção de resíduos de 13 antimicrobianos inoculados em leite isento de resíduos. Foram utilizadas quatro concentrações, sendo a primeira equivalente à metade do limite de detecção declarado pelo fabricante (C1; a segunda equivalente ao limite máximo de resíduos (LMR estabelecido pela legislação brasileira (C2; a terceira equivalente ao limite de detecção declarado pelo fabricante (C3; e a quarta equivalente ao dobro do limite de detecção declarado pelo fabricante (C4. Os testes apresentaram 100% de sensibilidade na detecção de amoxicilina, ampicilina, cloxacilina, penicilina, tilosina, sulfadiazina, sulfametoxazol, e 96,7% na detecção de oxacilina para as concentrações informadas pelo fabricante como limite de detecção. Em relação às concentrações estabelecidas pela legislação brasileira, verificou-se que os testes foram capazes de detectá-las para a maioria dos antimicrobianos avaliados. Os testes não se mostraram eficientes na detecção das concentrações estabelecidas pela legislação para eritromicina, oxitetraciclina, tetraciclina, trimetoprim e gentamicina (Microplate.The efficacy of the microbiological kits COPAN (Microplate and Single for detection of antimicrobials residues was tested. Thirteen drugs were inoculated into milk without residues at four concentration levels: C1 - half of the detection threshold declared by the manufacturer, C2 - the maximum residues threshold established by the Brazilian legislation, C3 - the detection threshold declared by the manufacturer, and C4 - two fold the detection threshold declared by the manufacturer. The tests showed 100% efficient in detection of amoxicillin, cloxacillin, penicillin, tilosin, sulfadiazine, and sulfa methoxazol; and 96.7% for oxacillin considering the concentration stated by the manufacturer as threshold level. Regarding the concentrations established by

  1. La lengua salvada: Acerca de dibaxu de Juan Gelman

    Directory of Open Access Journals (Sweden)

    Enrique Abel Foffani

    2000-11-01

    Full Text Available Se intenta leer el libro dibaxu de Juan Gelman escrito en sefardí desde el horizonte de la tradición poética latinoamericana. A través de la metáfora acerca de "la lengua salvada" de Elías Canetti (una imagen-aconteciªmiento de su propia autobiografía es posible reflexionar sobre la condición de judío y también sobre la relación de la lengua materna y la infancia. En la estela de Rubén Darío y de los poetas posteriores que se vieron en la situación de cambiar de lengua por diversos motivos, Juan Gelman elige el ladino o español sefardí como un doble extrañamiento de la lengua poética: por un lado pertenece a la línea azkenazí y no sefardita y por el otro recupera el español del siglo XV en consonancia con otras propuestas poéticas en las que había ya ensayado el rescate de la voz de poetas españoles como San Juan de la Cruz , Santa Teresa y los poetas místicos árabes. Desde esta perspectiva, el artículo analiza desde la extraterritorialización de la lengua poética los temas de dibaxu y otros libros anteriores a fin de señalar ciertas constantes y sus modos diferentes de articulación en el texto poético.

  2. La lengua salvada: Acerca de dibaxu de Juan Gelman

    Directory of Open Access Journals (Sweden)

    Enrique Abel Foffani

    2001-11-01

    Full Text Available Se intenta leer el libro dibaxu de Juan Gelman escrito en sefardí desde el horizonte de la tradición poética latinoamericana. A través de la metáfora acerca de "la lengua salvada" de Elías Canetti (una imagen-aconteciªmiento de su propia autobiografía es posible reflexionar sobre la condición de judío y también sobre la relación de la lengua materna y la infancia. En la estela de Rubén Darío y de los poetas posteriores que se vieron en la situación de cambiar de lengua por diversos motivos, Juan Gelman elige el ladino o español sefardí como un doble extrañamiento de la lengua poética: por un lado pertenece a la línea azkenazí y no sefardita y por el otro recupera el español del siglo XV en consonancia con otras propuestas poéticas en las que había ya ensayado el rescate de la voz de poetas españoles como San Juan de la Cruz , Santa Teresa y los poetas místicos árabes. Desde esta perspectiva, el artículo analiza desde la extraterritorialización de la lengua poética los temas de dibaxu y otros libros anteriores a fin de señalar ciertas constantes y sus modos diferentes de articulación en el texto poético.

  3. Animales en la obra de Juan José Arreola

    OpenAIRE

    Samperio, Guillermo

    2004-01-01

    Los bestiarios medievales son una tentativa, aunque sea parcial, por explicar la creación. En la letras latinoamericanas, Jorge Luis Borges, Julio Cortázar y Juan José Arreola renuevan los bestiarios de la edad Media. En este artículo se analizará la obra de Arreola, pues en ella, la imagen del animal trasciende de la forma simbólica del bestiario medieval y toma la forma nominalista del signo para la formulación de metáforas que transforman, no sólo a los hombres, sino también a los objetos,...

  4. Il mestiere di tradurre 1: entrevista a Juan Antonio Vivanco

    Directory of Open Access Journals (Sweden)

    Juan Antonio Vivanco Gefaell

    2013-01-01

    Full Text Available Hijo del poeta Luis Felipe Vivanco y de la escritora María Luisa Gefaell, Juan Antonio Vivanco Gefaell lleva más de tres décadas desarrollando una prestigiosa labor como traductor. Por sus manos han pasado autores italianos tan dispares como Roberto Saviano (La belleza y el infierno, Debolsillo, 2010, Paolo Maurensig (Canon inverso, Mondadori, 1997, Pier Paolo Pasolini (Escritos corsarios, Ed. Del Oriente, 2009 o Paolo Cottino (La ciudad imprevista, Bellaterra, 2005, por citar unos pocos.Traductor minucioso y preciso no sólo de textos narrativos, también se deben a él textos técnicos que abarcan media docena de especialidades humanísticas: desde  libros de antropología como el interesante Genes, pueblos y lengua de Luca Cavalli Sforza (Crítica, 1997, a textos de etnografía como La tierra del remordimiento de Ernesto de Martino (Bellaterra, 1999 en cuya traducción, nos comenta, disfrutó de forma especial, hasta llegar a densos manuales como la Historia de España de Joseph Pérez (Crítica, 1999 o Crisis e inflación entre la Antigüedad y la Edad Media de Georges Depeyrot (Crítica, 1996. Intentar abarcar el trabajo de Juan Antonio Vivanco implicaría, sin duda, citar las mejores editoriales de nuestro país y una amplia variedad temática hasta abarcar disciplinas tan diversas como la micología, el alpinismo, la historia del Arte y, especialmente, sus múltiples traducciones de estudios sobre Islam y Oriente Medio.Zibaldone. Estudios italianos le agradece que se haya prestado a inaugurar este espacio, Il mestiere di tradurre, contestando a unas preguntas sobre su trayectoria y su  profesión. Entrevista de Juan José Tejero y Juan Pérez Andrés. 

  5. Mito, peregrinación y modernidad en Juan Rulfo.

    OpenAIRE

    2014-01-01

    Aunque confiesa a Luis Harss (1) que se considera de San Gabriel, donde pasó su infancia, Juan Rulfo nació en Sayula, Jalisco, en 1918. Autor de un libro de cuentos, una novela y unos guiones cinematográficos, así como de expresivas fotografías que revelan la hondura de su alma,, de su tierra, y de su pueblo, se desempeño durante largo tiempo como empleado en el Instituto Indigenista de México.

  6. Juan Valdez in the coffe market of France

    OpenAIRE

    Rodriguez Maldonado, Edna Katherine

    2010-01-01

    El objetivo de este trabajo es presentar un marco organizacional en profundidad de las operaciones de Juan Valdez y sus posibilidades de incursionar en el mercado Francés. El Análisis se presenta en base a una investigación de mercados que tiene como referencia las tendencias de consumo, más exactamente hacía bebidas con alto contenido de cafeína, (Bebidas energizantes, deportivas, café etc.). Igualmente se analizan los socios estratégicos con más fuerza de realizar alianzas con la empresa c...

  7. Mediastinal pathology and the contributions of Dr. Juan Rosai.

    Science.gov (United States)

    Wick, Mark R

    2016-09-01

    Dr. Juan Rosai is one of the most prolific contributors to the literature on mediastinal pathology, and he has added steadily to that body of work over a 50-year period. Rosai has written several landmark articles in this topical area, including articles on thymic epithelial lesions, mediastinal neuroendocrine tumors, mediastinal lymphoma and other hematopoietic lesions, thymolipoma, thymoliposarcoma, mediastinal solitary fibrous tumor, intrathymic langerhans-cell histiocytosis, mediastinal germ cell neoplasms, and multilocular thymic cyst. This review recounts his role as one of the principal figures in the surgical pathology of mediastinal diseases.

  8. Juan Rodríguez Freile (cronista colonial

    Directory of Open Access Journals (Sweden)

    Julio Enrique Cuervo Escobar

    1963-06-01

    Full Text Available Juan Rodríguez Freile -1566-1638-. Aspirante al sacerdocio, soldado en los albores de la Conquista y más tarde hombre pacífico, que cambió las armas por instrumentos de labranza y en la vejez por la pluma, escribiendo sobre la "Conquista y Descubrimiento del Nuevo Reino. de Granada . .. " (El título completo equivale al índice 1, crónica santafereña que ha pasado a la posteridad con el título familiar de '·'EI Carnero".

  9. Iglesia de San Juan de Mayorga : un espacio recuperado

    OpenAIRE

    Valle González, Roberto

    2014-01-01

    El Museo del Pan ha sido diseñado a partir del edificio existente de la Iglesia de San Juan, en la localidad de Mayorga. La iglesia es de estilo mudéjar y estaba abandonada desde hace mucho tiempo. Siendo insuficiente su superficie para albergar los contenidos del museo, se hizo necesario construir un nuevo edificio en el solar disponible, adosado a la iglesia por el lado opuesto a la cabecera. El nuevo edificio queda así determinado por la forma del solar, el edificio adosado medianero y ...

  10. LA POÉTICA DE LA CONCIENCIA JUAN CARLOS MESTRE

    OpenAIRE

    CARLOS EDUARDO ORDÓÑEZ ORDÓÑEZ

    2015-01-01

    A trajetória poética de Juan Carlos Mestre (León, España, 1957) se urde em diversas geografias cujos contextos influem de forma determinante no seu pensamento poético: a visão melancólica e trágica de Villafranca del Bierzo, sua cidade natal; a agitada Barcelona dos movimentos de oposição ao franquismo, onde o poeta realizou estudos universitários; o âmbito chileno, entre utopia e desgraça, durante os últimos anos da ditadura de Augusto Pinochet; a Madri de finais dos anos oite...

  11. A morte e as mortes na obra de Juan Rulfo

    OpenAIRE

    Euzébio, Vilmar Machado

    2008-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Comunicação e Expressão. Programa de Pós-Graduação em Literatura A presente dissertação realiza uma trajetória pela narrativa de el Llano en Llamas (1953) e Pedro Páramo (1955) de Juan Rulfo. Nessas obras, a morte - grande conflito humano - aparece como tema recorrente. Os personagens de el Llano en Llamas "vivem"ensimesmados na solidão do campo, enquanto os personagens de Pedro Páramo sofrem coletivamente sob o dom...

  12. Rabia canina en san Juan de Lurigancho y en Jicamarca

    OpenAIRE

    2004-01-01

    Hasta la tercera semana de noviembre se han confirmado cuatro casos de rabia canina en la DISA Lima Norte, el último caso se presentó el 20 de noviembre en la granja porcina «Pradera del Paraíso» en el distrito de San Juan de Lurigancho (SJL), perteneciente a la referida Dirección de Salud. Dicho caso sucedió en momentos que un trabajador de la granja trató de separar la pelea entre los perros guardianes de la granja y un perro de origen desconocido, siendo finalmente mordido en una de...

  13. El realismo mágico en "Juan Girador"

    OpenAIRE

    Arguedas, María Eugenia

    2015-01-01

    El realismo mágico es un elemento vital en el "Juan girador" una de las leyendas en "El espejo de Lida Sal". Este análisis comprende el barroco y el mito y la leyenda, todos los cuales aparecen en diferentes niveles textuales, dando como resultado una creación literaria altamente inusual. La leyenda se analiza como una narración corta que se utiliza para presentar una realidad mítica. Se da especial importancia al uso de Asturias de un elemento puramente latinoamericano, el indio, como una co...

  14. Free vibration and biaxial buckling analysis of magneto-electro-elastic microplate resting on visco-Pasternak substrate via modified strain gradient theory

    Science.gov (United States)

    Jamalpoor, A.; Ahmadi-Savadkoohi, A.; Hosseini-Hashemi, Sh

    2016-10-01

    This paper deals with the theoretical analysis of free vibration and biaxial buckling of magneto-electro-elastic (MEE) microplate resting on Kelvin-Voigt visco-Pasternak foundation and subjected to initial external electric and magnetic potentials, using modified strain gradient theory (MSGT). Kirchhoff plate model and Hamilton’s principle are employed to extract the governing equations of motion. Governing equations were analytically solved to obtain clear closed-form expression for complex natural frequencies and buckling loads using Navier’s approach. Numerical results are presented to reveal variations of natural frequency and buckling load ratio of MEE microplate against different amounts of the length scale parameter, initial external electric and magnetic potentials, aspect ratio, damping and transverse and shear stiffness parameters of the visco-Pasternak foundation, length to thickness ratio, microplate thickness and higher modes. Numerical results of this study illustrate that by increasing thickness-to-material length scale parameter ratio, both natural frequency and buckling load ratio predicted by MSGT and modified couple stress theory are reduced because the non-dimensional length scale parameter tends to decrease the stiffness of structures and make them more flexible. In addition, results show that initial external electric and initial external magnetic potentials have no considerable influence on the buckling load ratio and frequency of MEE microplate as the microplate thickness increases.

  15. Long-term response on growth, antioxidant enzymes, and secondary metabolites in salicylic acid pre-treated Uncaria tomentosa microplants.

    Science.gov (United States)

    Sánchez-Rojo, Silvia; Cerda-García-Rojas, Carlos M; Esparza-García, Fernando; Plasencia, Javier; Poggi-Varaldo, Héctor M; Ponce-Noyola, Teresa; Ramos-Valdivia, Ana C

    2015-12-01

    To obtain micro propagated Uncaria tomentosa plantlets with enhanced secondary metabolites production, long-term responses to salicylic acid (SA) pre-treatments at 1 and 100 µM were evaluated after propagation of the plantlets in a SA-free medium. SA pre-treatments of single node cuttings OF U. tomentosa produced long-term responses in microplants grown for 75 days in a SA-free medium. Reduction in survival rate, root formation, and stem elongation were observed only with 100 µM SA pre-treatments with respect to the control (0 + DMSO).Both pre-treatments enhanced H2O2 and inhibited superoxide dismutase and catalase activities, while guaiacol peroxidase was increased only with 1 µM SA. Also, both pre-treatments increased total monoterpenoid oxindole alkaloids by ca. 55 % (16.5 mg g(-1) DW), including isopteropodine, speciophylline, mitraphylline, isomitraphylline, rhynchopylline, and isorhynchopylline; and flavonoids by ca. 21 % (914 μg g(-1) DW), whereas phenolic compounds were increased 80 % (599 μg g(-1) DW) at 1 µM and 8.2 % (359 μg g(-1) DW) at 100 µM SA. Pre-treatment with 1 µM SA of U.tomentosa microplants preserved the survival rate and increased oxindole alkaloids, flavonoids, and phenolic compounds in correlation with H2O2 and peroxidase activity enhancements, offering biotechnological advantages over non-treated microplants.

  16. A Microplate Growth Inhibition Assay for Screening Bacteriocins against Listeria monocytogenes to Differentiate Their Mode-of-Action.

    Science.gov (United States)

    Vijayakumar, Paul Priyesh; Muriana, Peter M

    2015-06-11

    Lactic acid bacteria (LAB) have historically been used in food fermentations to preserve foods and are generally-recognized-as-safe (GRAS) by the FDA for use as food ingredients. In addition to lactic acid; some strains also produce bacteriocins that have been proposed for use as food preservatives. In this study we examined the inhibition of Listeria monocytogenes 39-2 by neutralized and non-neutralized bacteriocin preparations (Bac+ preps) produced by Lactobacillus curvatus FS47; Lb. curvatus Beef3; Pediococcus acidilactici Bac3; Lactococcus lactis FLS1; Enterococcus faecium FS56-1; and Enterococcus thailandicus FS92. Activity differences between non-neutralized and neutralized Bac+ preps in agar spot assays could not readily be attributed to acid because a bacteriocin-negative control strain was not inhibitory to Listeria in these assays. When neutralized and non-neutralized Bac+ preps were used in microplate growth inhibition assays against L. monocytogenes 39-2 we observed some differences attributed to acid inhibition. A microplate growth inhibition assay was used to compare inhibitory reactions of wild-type and bacteriocin-resistant variants of L. monocytogenes to differentiate bacteriocins with different modes-of-action (MOA) whereby curvaticins FS47 and Beef3, and pediocin Bac3 were categorized to be in MOA1; enterocins FS92 and FS56-1 in MOA2; and lacticin FLS1 in MOA3. The microplate bacteriocin MOA assay establishes a platform to evaluate the best combination of bacteriocin preparations for use in food applications as biopreservatives against L. monocytogenes.

  17. Efeitos de microplástico na fisiologia do mexilhão Perna perna (Bivalvia: Mytilidae)

    OpenAIRE

    Liv Goldstein Ascer

    2015-01-01

    Dentre as diferentes poluições presentes nos oceanos, o plástico é provavelmente, aquele que leva aos maiores impactos ambientais, sendo encontrado de praias e manguezais à giros no meio dos oceanos. Os macroplásticos, cujo tamanho é superior a 5mm, são maiores em volume e provocam um impacto socioambiental importante, porém os microplásticos, fragmentos menores que 5mm, estão em maior quantidade e podem provocar danos em organismos marinhos filtradores como mexilhões e ostras. Apesar da sua ...

  18. Paralelismos y divergencias entre Juan Tenorio de Tirso de Molina y Miguel Páramo, personaje de Juan Rulfo.

    Directory of Open Access Journals (Sweden)

    Herlinda Ramírez-Barradas.

    2009-01-01

    Full Text Available Resumen: En este breve artículo se presentan algunas características de Miguel Páramo que, primero, permiten incluirlo en la larga lista de personajes donjuanescos derivados de la obra de Tirso de Molina y que, además, hacen posible entender su función trágica.Summary: This article presents some of Miguel Páramo's characteristics that, first, allow including him in the long list of prominent Don Juan figures derived from Tirso de Molina's work and, in addition, make it possible to understand his tragic function.

  19. Aprendizajes del Accidente de San Juan Ixhuatepec-México Learning from the Accident in San Juan Ixhuatepec-Mexico

    OpenAIRE

    Antioco López-Molina; Richart Vázquez-Román; Christian Díaz-Ovalle

    2012-01-01

    El índice Dow de fuego y explosión y la metodología del análisis cuantitativo del riesgo son aplicados para analizar uno de los accidentes más desafortunados en la historia mexicana: la explosión de tanques de almacenamiento de gas en la planta de San Juan Ixhuatepec en México. Estimaciones adicionales de la sobrepresión y radiación térmica producida durante el siniestro son llevadas a cabo para explicar el efecto dominó producido como consecuencia del incidente. Estas estimaciones dan eviden...

  20. La encrucijada en Pedro Páramo de Juan Rulfo / The Crossroads in Pedro Páramo by Juan Rulfo

    OpenAIRE

    Lilia Leticia García-Peña

    2015-01-01

    La obra de Juan Rulfo sintetiza la realidad del campo mexicano, captura el contorno de las identidades individuales y de la estructura de la interacción social del mundo que tanto le preocupó y del que es intérprete indiscutible, pero también supo convertir en materia narrativa aquellas atmósferas que rebasan el contexto mexicano y social. En este trabajo abordaré el tema de la encrucijada en el imaginario simbólico de Pedro Páramo (1955) como expresión de una reelaboración del mito de Hermes...

  1. Cuisine Preference of Local Tourists in San Juan, Batangas, Philippines

    Directory of Open Access Journals (Sweden)

    RYENE SELLINE B. KALALO

    2014-08-01

    Full Text Available This study aimed to determine the cuisine preference of the local tourist in San Juan, Batangas. More specifically, it aimed to describe the demographic profile of local tourist; to identify the preferred cuisine by different restaurants; to determine the significant difference when group according to demographic profile; and to determine the cuisine preference of local tourists in San Juan, Batangas. The research design used the descriptive method because it is the most appropriate method. It was found that the over-all assessment was frequent. Hamburger received the highest weighted mean followed by Sandwiches interpreted as frequent. Doughnut and Roasted Turkey got the lowest. Chinese Cuisine is frequently served. Lumpiang Shanghai has the highest weighted mean that is frequently offered and Siomai being the second highest. Siopao and Dumpling got the lowest weighted mean that makes it sometimes offered in every restaurant. Japanese cuisine has an over-all assessment of frequent. Tempura has the highest weighted mean followed by Teriyaki. Ramen has the second to the lowest weighted mean and Tonkatsu got the lowest. French Cuisine has a composite mean with an over-all assessment of sometimes. Mediterranean salad has the highest weighted followed by French Macaroons. Lamb and Ratatouille has the lowest weighted mean

  2. Juan O’Gorman. Formas de no ser arquitecto

    Directory of Open Access Journals (Sweden)

    Javier Jerez González

    2015-12-01

    Full Text Available La obra proyectada y construida por Juan O’Gorman a lo largo de su vida traza un recorrido largo y sincopado entre formas de entender la arquitectura totalmente antagónicas. Su primera etapa de radical racionalismo desarrolla, según procedimientos estrictamente técnicos, un lenguaje revolucionario basado en la austeridad y la precisión. A pesar de ello muchas de sus obras destacan por la plasticidad de sus composiciones volumétricas, la riqueza espacial o, incluso, por la creación de atmósferas oníricas. Los primeros años de frenética actividad dan paso a un periodo de voluntario alejamiento de la práctica profesional de la arquitectura: ni una sola obra, ni un solo proyecto durante cerca de 15 años. Tan solo su actividad docente le mantiene en contacto con el mundo de la arquitectura. Cuando Juan O’Gorman vuelve a ejercer como arquitecto, lo hace construyendo algunos de los iconos del movimiento de integración plástica de México: la biblioteca central de la UNAM y su casa en San Jerónimo. A pesar de las enormes diferencias, o más bien oposiciones, entre unos momentos y otros, puede identificarse una invariante clara como señal de identidad personal: una velada voluntad de no ser arquitecto

  3. Juan de Arfe Villafañe y Sebastiano Serlio

    Directory of Open Access Journals (Sweden)

    Heredia Moreno, Mª del Carmen

    2003-12-01

    Full Text Available Juan de Arfe's awareness and utilization of Italian art treatises, particularly that of Sebastiano Serlio, are analyzed in this study. The author demonstrates that Serlio's influence is perceptible in much of this artisan's production, from the Avila monstrance to that of Valladolid. It is especially manifest in Arfe's creations carried out in Seville, particularly in Books I and IV of his Varia Commensuracion and in the monstrance for the cathedral.

    En este artículo se analiza el conocimiento y la utilización de los tratadistas italianos, particularmente de Sebastiano Serlio, por parte de Juan de Arfe. También se tratará de demostrar que la influencia de Serlio se percibe en buena parte de la producción del artífice, desde la custodia de Ávila hasta la de Valladolid, aunque se manifiesta de manera especial en los trabajos que Arfe realizó en Sevilla, sobre todo en los Libros I y IV de la Varia Commensuracion y en la custodia de la catedral.

  4. SCAP optimization of the Pasajes de San Juan power station

    Energy Technology Data Exchange (ETDEWEB)

    Perez, L.; Perez, J.M.; Cerezo, J.; Catediano, J.; Martin-Sanchez, J.M. [Iberdrola, Bilbao (Spain)

    1995-08-01

    In July 1990 the company Iberduero, now Iberdrola, decided to carry out a development project using the Spanish technology SCAP in the Pasajes de San Juan power plant. This methodology, featured by the utilization of adaptive predictive control methodology, had been successfully applied in order to achieve the optimization of other industrial processes. The ultimate aim of this project was the replacement of the power station conventional control system, with a SCAP control system with a distributed structure able to optimize the electric energy generation process by optimizing power station operation, maximizing efficiency and working life, reducing maintenance costs, and increasing safety and availability. The project was divided into two stages to evaluate the quality of SCAP technology in the control and optimization of a power station. In the present report, the results obtained in the control of the most critical variables during the power station operation are gathered together. Beforehand, and in order to place these results in a suitable context, the project proceedings summarized in the following phases are considered: the SCAP technology; problems in a thermal power plant; and the Pasajes de San Juan power plant and its control system. The results are presented through comparative graphs that show the performance of the SCAP system as compared to the power station conventional control. 17 figs., 4 tabs., 11 refs.

  5. TRAFFIC SAFETY AND DRIVER EDUCATION IN SAN JUAN ARGENTINA

    Directory of Open Access Journals (Sweden)

    Arístides Osvaldo Fernández DE CIEZA

    2000-01-01

    Full Text Available With over 8,100 traffic fatalities in 1997 and an accident rate per 100 million vehicle kilometres travelled, approximately five times that of United States, Argentinean road authorities are now beginning to focus attention on traffic safety and driver education. One of the main problems in the search of causes for car accidents in Argentina is the lack of a reliable and updated data base. The results and conclusions presented in this paper are based on a thorough analysis of car accidents in the Province of San Juan, Argentina. A seven-year data base of car accidents has been compiled from police reports, including the results of traffic counts at intersections and other collision locations. In addition, topographic and filmed reports of such places and their surroundings bring about parameters such as stop lines, visibility triangles, road size, traffic light performance, etc., which allow to carrying out of a traffic flow analysis for proposing measures aiming to minimize accidents. For San Juan province, in general, the main causes are: high absolute car speeds, speed differences between vehicles, lack of good lighting, poor driving habits, lack of traffic control devices such as signs, signals, and an absence of road markings.

  6. HNO₃-assisted polyol synthesis of ultralarge single-crystalline Ag microplates and their far propagation length of surface plasmon polariton.

    Science.gov (United States)

    Chang, Cheng-Wei; Lin, Fan-Cheng; Chiu, Chun-Ya; Su, Chung-Yi; Huang, Jer-Shing; Perng, Tsong-Pyng; Yen, Ta-Jen

    2014-07-23

    We developed a HNO3-assisted polyol reduction method to synthesize ultralarge single-crystalline Ag microplates routinely. The edge length of the synthesized Ag microplates reaches 50 μm, and their top facets are (111). The mechanism for dramatically enlarging single-crystalline Ag structure stems from a series of competitive anisotropic growths, primarily governed by carefully tuning the adsorption of Ag(0) by ethylene glycol and the desorption of Ag(0) by a cyanide ion on Ag(100). Finally, we measured the propagation length of surface plasmon polaritons along the air/Ag interface under 534 nm laser excitation. Our single-crystalline Ag microplate exhibited a propagation length (11.22 μm) considerably greater than that of the conventional E-gun deposited Ag thin film (5.27 μm).

  7. Facile preparation of glycoprotein-imprinted 96-well microplates for enzyme-linked immunosorbent assay by boronate affinity-based oriented surface imprinting.

    Science.gov (United States)

    Bi, Xiaodong; Liu, Zhen

    2014-01-01

    Molecularly imprinted polymers (MIPs), as inexpensive and stable substitutes of antibodies, have shown great promise in immunoassays. Glycoproteins are of significant diagnostic value. To facilitate the application of MIPs in clinical diagnostics, a general and facile imprinting method toward glycoproteins oriented for an enzyme-linked immunosorbent assay (ELISA) in the form of a 96-well microplate is essential but has not been fully explored yet. In this study, a new method called boronate affinity-based oriented surface imprinting was proposed for facile preparation of glycoprotein-imprinted microplates. A template glycoprotein was first immobilized by a boronic acid-modified microplate through boronate affinity binding, and then, a thin layer of polyaniline was formed to cover the microplate surface via in-water self-copolymerization. After the template was removed by an acidic solution, 3D cavities that can rebind the template were fabricated on the microplate surface. Using horseradish peroxidase (HRP) as a model target, the effects of imprinting conditions as well as the properties and performance of the prepared MIPs were investigated. α-Fetoprotein (AFP)-imprinted microplate was then prepared, and thereby, a MIP-based ELISA method was established. The prepared MIPs exhibited several highly favorable features, including excellent specificity, widely applicable binding pH, superb tolerance for interference, high binding strength, fast equilibrium kinetics, and reusability. The MIP-based ELISA method was finally applied to the analysis of AFP in human serum. The result was in good agreement with that by radioimmunoassay, showing a promising prospect of the proposed method in clinical diagnostics.

  8. Paleogene and Neogene magmatism in the Valle del Cura region: New perspective on the evolution of the Pampean flat slab, San Juan province, Argentina

    Science.gov (United States)

    Litvak, Vanesa D.; Poma, Stella; Kay, Suzanne Mahlburg

    2007-09-01

    The Valle del Cura region is characterized by a thick volcanic and volcaniclastic sequence that records the Tertiary arc and backarc magmatic evolution of the Argentine Main Cordillera over the modern Pampean flatslab at 29.5-30°S. During the Eocene, a retroarc basin developed, represented by the Valle del Cura Formation synorogenic volcanosedimentary sequence, which includes rhyolites and dacitic tuffs. These silicic volcanic rocks have weak arc chemical signatures and high lithophile element concentrations and are isotopically enriched relative to the late Oligocene-early Miocene volcanic rocks that followed them. Their chemical characteristics fit with eruption through a thin crust. The Valle de Cura Formation was followed by the Oligocene-early Miocene Doña Ana Group volcanic sequence, which erupted at and near the arc front west of the border with Chile. The Doña Ana Group volcanic rocks have calc-alkaline chemical characteristics consistent with parental magmas forming in a mantle wedge and erupting through a normal thickness crust (35 km). Subsequent shallowing of the downgoing Nazca plate caused the volcanic front to migrate eastward. The volcanic sequences of the middle Miocene Cerro de las Tórtolas Formation erupted at this new arc front, essentially at the Argentine border. Two stages are recognized: an older one (16-14 Ma) in which magmas appear to have erupted through a normal thickness crust (30-35 km) and a younger one (13-10 Ma) in which the steeper REE pattern suggests the magmas last equilibrated with higher pressure residual mineral assemblages in a thicker crust. Isotopic ratios in the younger group are consistent with an increase in original crustal components and crust introduced into the mantle source by forearc subduction erosion. A peak in forearc subduction erosion near 12-10 Ma is consistent with when the main part of the Juan Fernandez Ridge began to subduct beneath the region. In addition to late Miocene Tambo Formation dacitic

  9. Space-time model for migration of weak earthquakes along the northern boundary of the Amurian microplate

    Science.gov (United States)

    Trofimenko, S. V.; Bykov, V. G.; Merkulova, T. V.

    2016-07-01

    In this paper, we aimed to investigate the statistical distributions of shallow earthquakes with 2 ≤ M ≤ 4, located in 13 rectangular areas (clusters) bounded by 120°E and 144°E along the northern boundary of the Amurian microplate. As a result of our study, the displacement of seismicity maxima has been determined and three recurrent spatial cycles have been observed. The clusters with similar distribution of earthquakes are suggested to alternate being equally spaced at 7.26° (360-420 km). A comparison of investigation results on the structure of seismicity in various segments of the Amurian microplate reveals the identity between the alternation pattern observed for meridional zones of large earthquakes and a distinguished spatial period. The displacement vector for seismicity in the annual cycles is determined, and the correspondence between its E-W direction and the displacement of the fronts of large earthquakes is established. The elaborated model of seismic and deformation processes is considered, in which subsequent activation of clusters of weak earthquakes (2 ≤ M ≤ 4), tending to extend from the Japanese-Sakhalin island arc to the eastern closure of the Baikal rift zone, is initiated by the displacement of the strain wave front.

  10. A Colorimetric Microplate Assay for DNA-Binding Activity of His-Tagged MutS Protein.

    Science.gov (United States)

    Banasik, Michał; Sachadyn, Paweł

    2016-09-01

    A simple microplate method was designed for rapid testing DNA-binding activity of proteins. The principle of the assay involves binding of tested DNA by his-tagged protein immobilized on a nickel-coated ELISA plate, following colorimetric detection of biotinylated DNA with avidin conjugated to horseradish peroxidase. The method was used to compare DNA mismatch binding activities of MutS proteins from three bacterial species. The assay required relatively low amounts of tested protein (approximately 0.5-10 pmol) and DNA (0.1-10 pmol) and a relatively short time of analysis (up to 60 min). The method is very simple to apply and convenient to test different buffer conditions of DNA-protein binding. Sensitive colorimetric detection enables naked eye observations and quantitation with an ELISA reader. The performance of the assay, which we believe is a distinguishing trait of the method, is based on two strong and specific molecular interactions: binding of a his-tagged protein to a nickel-coated microplate and binding of biotinylated DNA to avidin. In the reported experiments, the solution was used to optimize the conditions for DNA mismatch binding by MutS protein; however, the approach could be implemented to test nucleic acids interactions with any protein of interest.

  11. An optimized microplate assay system for quantitative evaluation of plant cell wall-degrading enzyme activity of fungal culture extracts.

    Science.gov (United States)

    King, Brian C; Donnelly, Marie K; Bergstrom, Gary C; Walker, Larry P; Gibson, Donna M

    2009-03-01

    Developing enzyme cocktails for cellulosic biomass hydrolysis complementary to current cellulase systems is a critical step needed for economically viable biofuels production. Recent genomic analysis indicates that some plant pathogenic fungi are likely a largely untapped resource in which to prospect for novel hydrolytic enzymes for biomass conversion. In order to develop high throughput screening assays for enzyme bioprospecting, a standardized microplate assay was developed for rapid analysis of polysaccharide hydrolysis by fungal extracts, incorporating biomass substrates. Fungi were grown for 10 days on cellulose- or switchgrass-containing media to produce enzyme extracts for analysis. Reducing sugar released from filter paper, Avicel, corn stalk, switchgrass, carboxymethylcellulose, and arabinoxylan was quantified using a miniaturized colorimetric assay based on 3,5-dinitrosalicylic acid. Significant interactions were identified among fungal species, growth media composition, assay substrate, and temperature. Within a small sampling of plant pathogenic fungi, some extracts had crude activities comparable to or greater than T. reesei, particularly when assayed at lower temperatures and on biomass substrates. This microplate assay system should prove useful for high-throughput bioprospecting for new sources of novel enzymes for biofuel production.

  12. Development of a simple fluorescence-based microplate method for the high-throughput analysis of proline in wine samples.

    Science.gov (United States)

    Robert-Peillard, Fabien; Boudenne, Jean-Luc; Coulomb, Bruno

    2014-05-01

    This paper presents a simple, accurate and multi-sample method for the determination of proline in wines thanks to a 96-well microplate technique. Proline is the most abundant amino acid in wine and is an important parameter related to wine characteristics or maturation processes of grape. In the current study, an improved application of the general method based on sodium hypochlorite oxidation and o-phthaldialdehyde (OPA)-thiol spectrofluorometric detection is described. The main interfering compounds for specific proline detection in wines are strongly reduced by selective reaction with OPA in a preliminary step under well-defined pH conditions. Application of the protocol after a 500-fold dilution of wine samples provides a working range between 0.02 and 2.90gL(-1), with a limit of detection of 7.50mgL(-1). Comparison and validation on real wine samples by ion-exchange chromatography prove that this procedure yields accurate results. Simplicity of the protocol used, with no need for centrifugation or filtration, organic solvents or high temperature enables its full implementation in plastic microplates and efficient application for routine analysis of proline in wines. Copyright © 2013 Elsevier Ltd. All rights reserved.

  13. A microplate reader-based method to quantify NADH-cytochrome b5 reductase activity for diagnosis of recessive congenital methaemoglobinemia.

    Science.gov (United States)

    Kedar, Prabhakar; Desai, Anand; Warang, Prashant; Colah, Roshan

    2017-05-01

    Congenital methemoglobinemia due to NADH-cytochrome b5 reductase 3 (CYB5R3) deficiencies is an autosomal recessive disorder that occurs sporadically worldwide, A sensitive, accurate, and rapid analysis of NADH-CYB5R enzyme concentrations is necessary for the diagnosis of RCM. Here we present an alternative microplate method that is based on a standard 96-well microplate format and microplate reader that simplify the quantification of NADH-CYB5R activity. TECAN (Infinite 200 PRO series) microplate reader with Tecan's proven Magellan™ software measured the NADH-CYB5R enzyme activity in 250 normal controls and previously diagnosed 25 cases of RCM due to NADH-CYB5R deficiency in the Indian population using 96-well microplates using 200 μl of total reaction mixture and also compared with standard spectrophotometric assay. We have also studied stability of the hemolysate stored at 4 and -20°C temperature. Enzyme activity in all 25 samples ranged from 6.09 to 10.07 IU/g Hb (mean ± SD: 8.08 ± 1.99 IU/g Hb) where as normal control ranged (n = 250) between 13.42 and 21.58 IU/g Hb) (mean ± SD: 17.5 ± 4.08 IU/g of Hb). Data obtained from the microplate reader were compared with standard spectrophotometer method and found 100% concordance using both methods. Microplate method allows differentiating between normal, deficient and intermediate enzyme activity. It was observed that samples had significant loss of activity when stored at 4°C and retained stable activity at -20°C for 1 week time. Our new method, incorporating a whole process of enzyme assay into a microplate format is readily applicable and allows rapid monitoring of enzyme assay. It is readily applicable to quantitative assay on pediatric sample as well as large number of samples for population screening.

  14. Bedrock aquifers of eastern San Juan County, Utah

    Science.gov (United States)

    Avery, Charles

    1986-01-01

    This study is one of a series of studies appraising the waterbearing properties of the Navajo Sandstone and associated formations in southern Utah.  The study area is about 4,600 square miles, extending from the Utah-Arizona State line northward to the San Juan-Grand County line and westward from the Utah-Colorado State line to the longitude of about 109°50'.Some of the water-yielding formations are grouped into aquifer systems. The C aquifer is comprised of the DeChelly Sandstone Member of the Cutler Formation.  The P aquifer is comprised of the Cedar Mesa Member of the Cutler Formation and the undifferentiated Cutler Formation. The N aquifer is comprised of the sedimentary section that includes the Wingate Sandstone, Kayenta Formation, Navajo Sandstone, Carmel Formation, and Entrada sandstone.  The M aquifer is comprised of the Bluff Sandstone Member and other sandstone units of the Morrison Formation.  The D aquifer is comprised of the Burro Canyon Formation and Dakota Sandstone.  Discharge from the ground-water reservoir to the San Juan River between gaging stations at Four Corners and Mexican Hat is about 66 cubic feet per second.The N aquifer is the main aquifer in the study area. Recharge by infiltration of precipitation is estimated to be 25,000 acre-feet per year.  A major ground-water divide exists under the broad area east of Monticello.  The thickness of the N aquifer, where the sedimentary section is fully preserved and saturated, generally is 750 to 1,250 feet.   Hydraulic conductivity values obtained from aquifer tests range from 0.02 to 0.34 foot per day.  The total volume of water in transient storage is about 11 million acre-feet. Well discharge somewhat exceeded 2,340 acre-feet during 1981.  Discharge to the San Juan River from the N aquifer is estimated to be 6.9 cubic feet per second. Water quality ranges from a calcium bicarbonate to sodium chloride type water

  15. Modelación de la conectividad de las subpoblaciones de la langosta de Juan Fernández (Jasus frontalis, a través de un modelo biofísico Modeling the connectivity of Juan Fernández rock lobster (Jasus frontalis, subpopulations through a biophysical model

    Directory of Open Access Journals (Sweden)

    Javier Porobic

    2012-09-01

    of Juan Fernandez and a considerable amount of larvae in the Desventuradas islands is proposed. These fluxes are determined mainly by the coupling between prevailing biological and oceanographic factors.

  16. Norma y uso: las ideas educativas en Juan de Robles / Etymology and use: The educational ideas of Juan de Robles

    Directory of Open Access Journals (Sweden)

    Alejandro Gómez Camacho

    2014-10-01

    Full Text Available Resumen: Juan de Robles es uno de los más brillantes humanistas de nuestro Siglo de Oro, entre cuyas obras destaca El culto sevillano (1631. En este trabajo se analizan sus ideas educativas, centradas en la relación entre la ortografía y el aprendizaje de la escritura. Entre las ideas pedagógicas de Juan de Robles es muy relevante el rechazo vehemente de la ortografía fonética, entre otros motivos, porque no favorecía el aprendizaje de la lectura y de la escritura; en consecuencia, censura la creación de nuevas letras y de un nuevo orden para el alfabeto español. Los ortógrafos defensores de la ortografía fonética en el siglo XVII argumentaban que la nueva escritura permitiría a los niños aprender a leer y a escribir con más rapidez y facilidad; así como que la creación de nuevas letras y el establecimiento de un nuevo orden del alfabeto facilitarían su enseñanza. Frente a estas pretensiones, Juan de Robles defendió el alfabeto tradicional y la ortografía etimológica de origen latino porque eran los instrumentos adecuados para la alfabetización en nuestra lengua. También critica los colegios jesuitas y la Ratio Studiorum; las principales discrepancias entre la pedagogía de la Compañía de Jesús y las ideas educativas de Juan de Robles podrían concretarse en la primacía del español sobre el latín, y en el uso de cuentos y refranes populares para la educación en El culto sevillano. En conclusión, con la obra de Juan de Robles se incorpora definitivamente el argumento pedagógico que relaciona la ortografía y la gramática españolas con el aprendizaje de la lectura y de la escritura a la polémica sobre la ortografía española del Siglo de Oro, en defensa de la ortografía etimológica frente a las pretensiones de imponer una ortografía fonética en el primer tercio del siglo XVII. Abstract: Juan de Robles is considered one of the main humanists in the Spanish Golden Century. The most important of his works is

  17. Urban ecology of Triatoma infestans in San Juan, Argentina

    Directory of Open Access Journals (Sweden)

    Silvana L Vallvé

    1996-08-01

    Full Text Available This study was performed in an urban neighborhood of the capital city of the province of San Juan, Argentina. Erected as a housing complex, the place consists of 768 flats distributed in buildings of three and seven floors each. A survey was carried out in 33% of the dwellings, enquiring about the number of Triatoma infestans found indoors, stage of the bug development - nymph or adult - and how these insects had entered their homes. Adult T.infestans were found on all floors; 163 people (64% had found them at least once, and 130 (51% several times. Dispersal flight seems to have been the main mechanism of infestation by adult bugs in this area, and a total of 51% of the surveyed inhabitants reported that the insects had flown into their flats.

  18. Central San Juan caldera cluster: regional volcanic framework

    Science.gov (United States)

    Lipman, Peter W.

    2000-01-01

    Eruption of at least 8800 km3 of dacitic-rhyolitic magma as 9 major ash-slow sheets (individually 150-5000 km3) was accompanied by recurrent caldera subsidence between 28.3 and about 26.5 Ma in the central San Juan Mountains, Colorado. Voluminous andesitic-decitic lavas and breccias were erupted from central volcanoes prior to the ash-flow eruptions, and similar lava eruptions continued within and adjacent to the calderas during the period of explosive volcanism, making the central San Juan caldera cluster an exceptional site for study of caldera-related volcanic processes. Exposed calderas vary in size from 10 to 75 km in maximum diameter, the largest calderas being associated with the most voluminous eruptions. After collapse of the giant La Garita caldera during eruption if the Fish Canyon Tuff at 17.6 Ma, seven additional explosive eruptions and calderas formed inside the La Garita depression within about 1 m.y. Because of the nested geometry, maximum loci of recurrently overlapping collapse events are inferred to have subsided as much as 10-17 km, far deeper than the roof of the composite subvolcanic batholith defined by gravity data, which represents solidified caldera-related magma bodies. Erosional dissection to depths of as much as 1.5 km, although insufficient to reach the subvolcanic batholith, has exposed diverse features of intracaldera ash-flow tuff and interleaved caldera-collapse landslide deposits that accumulated to multikilometer thickness within concurrently subsiding caldera structures. The calderas display a variety of postcollapse resurgent uplift structures, and caldera-forming events produced complex fault geometries that localized late mineralization, including the epithermal base- and precious-metal veins of the well-known Creede mining district. Most of the central San Juan calderas have been deeply eroded, and their identification is dependent on detailed geologic mapping. In contrast, the primary volcanic morphology of the

  19. Lengua y conocimiento en Juan de Valdés

    OpenAIRE

    2011-01-01

    Na presente pesquisa, é abordada a concepção de língua e conhecimento presente na atividade do biblicista espanhol Juan de Valdés (Cuenca, ca. 1500- Nápoles, 1541). Com tal fim, são analisados os critérios estilísticos propostos pelo autor no Diálogo de la Lengua, composto em torno de 1535 e dedicado ao uso e aprendizado da língua vernácula. Adotando como fundamentação teórica a hermenêutica do filósofo alemão Hans-George Gadamer e realizando uma revisão dos principais estudos sobre o autor e...

  20. Juan Manuel Roca: desde la patria de la infancia

    Directory of Open Access Journals (Sweden)

    Guillermo Linero Montes

    2003-05-01

    Full Text Available Desde que publicó Memoria del agua, su primer libro de poesía, en 1973, Juan Manuel Roca (Medellín, 1946 ha sido uno de los poetas colombianos más representativos de su época. Su reputación, alentada como ninguna otra por el interés de los jóvenes, tiene la singularidad de provenir de un hecho afortunado: de la exacta simbiosis entre los contenidos, la presentación y expresión de su poesía, y entre las necesidades espirituales de una sociedad en crisis. Un maridaje tanto acorazado como maleable, pues le ha permitido resistir, asimilándolas, las cardiacas transformaciones recientes.

  1. Diagnostic electron microscopy and the influence of Dr. Juan Rosai.

    Science.gov (United States)

    Wick, Mark R

    2016-09-01

    Transmission electron microscopy (TEM) was introduced by Ruska and Knoll as a laboratory technique in 1933. Thereafter, several decades passed before the methods required for its optimal implementation were fully developed. Early uses of TEM were in Botany, rather than in Medicine; however, isolated publications did catalog the ultrastructural characteristics of several individual human tumor types. Finally, in 1968, Rosai and Rodriguez authored an important article, introducing the concept that TEM could be used for the differential diagnosis of histologically similar neoplasms. This publication heralded the steadily increasing application of TEM in anatomic pathology over the following decade, including continuing contributions by Dr. Juan Rosai. This brief review summarizes his influence on clinical electron microscopy, and lists some of the lesions for which that procedure is still a useful means of analysis.

  2. Thermal performance of neighbourhood prototypes built in San Juan, Argentina

    Energy Technology Data Exchange (ETDEWEB)

    Blasco Lucas, I.; Albarracin, O.; Carestia, C. [Universidad Nacional de San Juan (Argentina)

    2000-07-01

    The research work produces a contrasted quantification of the thermal performance of inhabited dwelling houses built in different neighborhoods of San Juan, Argentina. They correspond to five prototypes aimed at families with low and medium income levels, massive spread in their versions with two or three bedrooms. From all of them it can be obtained yearly energy consumption indexes per unit area, under homogeneous conditions respect to minimal thermal comfort levels and inhabitant behaviors. The evolution of their inner temperatures and relative humidity with and without auxiliary thermal conditioning is analyzed. Conclusions assess the deficit level of the studied prototypes contrasted to international standards and the global valuation of the impact on the sectorial annual energy consumption caused by them. (author)

  3. Juan Antonio Rubio appointed as Director-General of CIEMAT

    CERN Multimedia

    2004-01-01

    Juan Antonio Rubio, Head of CERN's ETT unit (Education and Technology Transfer) has been appointed by the Spanish Ministry of Education and Science as the Director General of the Research Centre for Energy, Environment and Technology, CIEMAT. Dr Rubio's career began at the Spanish Nuclear Energy Commission where he held the posts of Investigator, Head of the High Energy Group and Head of Nuclear Physics and High Energy Division. Later, he was named Director of the Department of Basic Investigation and Scientific Director of the CIEMAT. In 1987 he joined CERN as Scientific Adviser to the Director General and Group Leader of the Scientific Assessment Group. Up to now, Dr Rubio has been the Head of the ETT unit, as well as Coordinator for Latin America and Commissioner for the 50th Anniversary of the Organization. He was born on 4 June 1944 in Madrid, and holds a Doctorate in Physical Sciences from the Universidad Complutense de Madrid.

  4. Sea level Variability and Juan de Fuca Bathymetry

    Science.gov (United States)

    Huybers, P. J.; Boulahanis, B.; Proistosescu, C.; Langmuir, C. H.; Carbotte, S. M.; Katz, R. F.

    2015-12-01

    That deglaciation influences mid-ocean ridge volcanism is well established for Iceland, where depressurization associated with melting a ~2 km ice cap led to order of magnitude increases in volcanism during the last deglaciation. The case was also made that the more subtle ~100 m changes in sea level that accompany glacial cycles have identifiable implications for undersea mid-ocean ridge systems using both models and data from the Australian-Antarctic Ridge (Crowley et al., 2015). Sea level rising at ~1 cm/year during deglaciation leads to an expectation of ~10% decreases in melt production at ridges, given mantle upwelling rates of ˜3 cm/yr at intermediate spreading ridges and mantle density being ~3 times that of seawater. The implications of variations in melt production for bathymetry, however, involve numerous considerations, including whether melt signals are cancelled within the melt column, appreciably alter accretionary or fault processes, and have identifiable surface expressions. Further empirical assessment of bathymetry is thus useful for purposes of confirming patterns and constraining processes. Here we report on spectral analyses of bathymetry recently acquired from the Juan de Fuca ridge between 44°30'N and 45°15'N during the SeaVOICE expedition. Multibeam swath sonar data were acquired with an EM122 sonar insonfiying seafloor to crustal ages of ˜2 ma with 35 m spatial resolution. We examine (1.) the statistical significance of concentrations of bathymetric variability at the 100 ky, 41 ky, and 23 ky periods characteristic of late-Pleistocene sea level variability; (2.) whether sea level responses are primarily at 41 ky periods in crust accreted during the early Pleistocene, when global sea level variations were primarily at this period; and (3.) if sea level responses are superimposed on bathymetry variations or, instead, align with fault features. We also note that Juan de Fuca's proximity to the Cordilleran Ice Sheet implies that regional

  5. La visión exiliar de Juan Gelman

    Directory of Open Access Journals (Sweden)

    María Ángeles PÉREZ LÓPEZ

    2009-11-01

    Full Text Available RESUMEN: En el poeta argentino Juan Gelman (1930, la traumática experiencia del exilio no viene a conformar sólo una parte importante de su producción, la comprendida entre 1975 y 1988, sino que además, y fundamentalmente, establece el desplazamiento hacia zonas de sentido en las que el lenguaje es también «el expulsado», el «vacío-pasión», «la marca de una ausencia que no cesa de no escribirse», en sus propios términos. De ahí que resulte tan relevante su encuentro con la poesía mística española, en la medida en que el místico es un exiliado de Dios (Citas y Comentarios, 1982, y con la poesía sefardí (Dibaxu, 1994, «como si la soledad extrema del exilio me empujara a buscar raíces en la lengua, las más profundas y exiliadas de la lengua».ABSTRACT: In the life of the Argentine poet Juan Gelman (1930, the traumatic experience of exile informs not only an important part of his production, the one that falls between 1975 and 1988, but also, and fundamentaly, it establishes the displacement to areas of significance in which language is also «the rejected», the «empty-passion», «the sign of an absence that does not finish writing itself», in his own words. From this, one discovers the importance of his encounter with Spanish mystical poetry, to the extent that the mystic is estranged from God (Citations and Comentaries, 1982 and with Sephardic poetry (Dibaxu, 1994, «as if the extreme solitude of exile compelled me to search for roots in language, the deepest and most estranged from language».

  6. San Juan Basin, CO and NM coal resources calculation area (sjbbndg)

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This shapefile and polygon coverage outline the area underlain by the Fruitland Formation in the San Juan Basin, Colorado and New Mexico. Also, it delimits the area...

  7. Final unioned polygon coverage used in coal resource calculations, San Juan Basin, CO and NM (sjbfing)

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This is a shapefile and the final unioned polygon coverage used to calculate coal resources of the Fruitland Formation, San Juan Basin coal assessment area, Colorado...

  8. Letter to Silverton and San Juan County Regarding Potential Superfund Listing

    Science.gov (United States)

    Feb. 12, 2016 Update: EPA added a letter to the Town of Silverton and San Juan County regarding the agency’s commitment to the Town and County’s involvement during a potential Superfund listing process.

  9. 33 CFR 165.758 - Security Zone; San Juan, Puerto Rico.

    Science.gov (United States)

    2010-07-01

    ... yards around all cruise ships entering, departing, moored or anchored in the Port of San Juan, Puerto... granted, all persons and vessels must comply with the instructions of the Captain of the Port or...

  10. La Reforma Universitaria como kulturkampf. La lectura gramsciana de Juan Carlos Portantiero

    National Research Council Canada - National Science Library

    Bustelo, Natalia

    2013-01-01

    .... Compartiendo ese interes, el presente trabajo se propone analizar las novedades introducidas por la lectura gramsciana de la Reforma Universitaria que propone Juan Carlos Portantiero en la decada del setenta...

  11. Sobre el nacimiento y procedencia de San Juan de Dios y su obra

    Directory of Open Access Journals (Sweden)

    Martínez Gil, José Luis

    2006-06-01

    Full Text Available This article studies the figure of St. Juan de Dios inside the historical context of Granada during the middle of XVIth century. The article tackles, from a chronological perspective, the main subjects of the biography of St. Juan and this assitencial work with poors and ills. The author goes more deeply into the situation of the hospitals in Granada in St. Juan de Dios day.Estudio de la figura de san Juan de Dios enmarcado en el contexto histórico de la Granada de mediados del siglo XVI. Se abordan, desde una perspectiva cronológica, los principales aspectos de la biografía del santo, así como su obra asistencial respecto a pobres y enfermos, profundizando en la situación de los hospitales en Granada en este período.

  12. Don Juan Basin, Wright Valley, Antarctica: Model for Surface Processes on Mars

    Science.gov (United States)

    Englert, P.; Bishop, J. L.; Gibson, E. K.; Patel, S.; Koeberl, C.

    2014-09-01

    Mineral, chemical, and soluble salt composition of drill core samples from Don Juan Basin, Wright Valley, Antarctica, indicate that the formation of the most saline terrestrial pond may include groundwater discharge and near surface flow processes.

  13. Metabolic effects of azoxystrobin and kresoxim-methyl against Fusarium kyushuense examined using the Biolog FF MicroPlate.

    Science.gov (United States)

    Wang, Hancheng; Wang, Jin; Chen, Qingyuan; Wang, Maosheng; Hsiang, Tom; Shang, Shenghua; Yu, Zhihe

    2016-06-01

    Azoxystrobin and kresoxim-methyl are strobilurin fungicides, and are effective in controlling many plant diseases, including Fusarium wilt. The mode of action of this kind of chemical is inhibition of respiration. This research investigated the sensitivities of Fusarium kyushuense to azoxystrobin and kresoxim-methyl, and to the alternative oxidase inhibitor salicylhydroxamic acid (SHAM). The Biolog FF MicroPlate is designed to examine substrate utilization and metabolic profiling of micro-organisms, and was used here to study the activity of azoxystrobin, kresoxim-methyl and SHAM against F. kyushuense. Results presented that azoxystrobin and kresoxim-methyl strongly inhibited conidial germination and mycelial growth of F. kyushuense, with EC50 values of 1.60 and 1.79μgml(-1), and 6.25 and 11.43μgml(-1), respectively; while not for SHAM. In the absence of fungicide, F. kyushuense was able to metabolize 91.6% of the tested carbon substrates, including 69 effectively and 18 moderately. SHAM did not inhibit carbon substrate utilization. Under the selective pressure of azoxystrobin and kresoxim-methyl during mycelial growth (up to 100μgml(-1)) and conidial germination (up to 10μgml(-1)), F. kyushuense was unable to metabolize many substrates in the Biolog FF MicroPlate; while especially for carbon substrates in glycolysis and tricarboxylic acid cycle, with notable exceptions such as β-hydroxybutyric acid, y-hydroxybutyric acid, α-ketoglutaric acid, α-d-glucose-1-phosphate, d-saccharic acid and succinic acid in the mycelial growth stage, and β-hydroxybutyric acid, y-hydroxybutyric acid, α-ketoglutaric acid, tween-80, arbutin, dextrin, glycerol and glycogen in the conidial germination stage. This is a new finding for some effect of azoxystrobin and kresoxim-methyl on carbon substrate utilization related to glycolysis and tricarboxylic acid cycle and other carbons, and may lead to future applications of Biolog FF MicroPlate for metabolic effects of other

  14. Late Quaternary Activity and Seismogenic Potential of the Gonave Microplate: Plantain Garden Strike-Slip Fault Zone of Eastern Jamaica

    Science.gov (United States)

    Mann, P.; Prentice, C.; King, W.; Demets, C.; Wiggins-Grandison, M.; Benford, B.

    2008-12-01

    At the longitude of Jamaica, Caribbean (Carib)-North America (Noam) plate motion of 19 ± 2 mm/a is carried by two parallel, left-lateral strike-slip faults, the Oriente fault zone, immediately south of Cuba, and the Enriquillo-Plantain Garden fault zone (EPGFZ), which lies 100-150 km further south. It has been postulated that the lithosphere between these faults constitutes an independent Gonave microplate that has formed in response to the ongoing collision between the leading edge of Carib in Hispaniola and the Bahama carbonate platform. GPS measurements in Jamaica and Hispanola is supportive of the microplate hypothesis and indicates that roughly half of Carib-Noam plate motion (8-14 mm/a) is carried by the EPGFZ of southern Hispaniola and eastern Jamaica. This study applies geomorphic and paleoseismic methods as a direct test of the activity and amount of microplate motion carried on the Plantain Garden fault segment of eastern Hispaniola and how this motion is distributed across a large restraining bend that has formed the island of Jamaica since the late Miocene. The EPFZ curves gently to the northeast and forming a steep mountain front to the Blue Mountains restraining bend with elevations up to 2200 m. Geomorphic fault-related features along the mountain front fault zone include left-laterally deflected rivers and streams, but no small scale features indicative of Holocene activity. River and stream deflections range from 0.1 to 0.5 km. We identified and trenched the most active trace of the mountain front fault at the Morant River where the fault is characterized by a 1.5-m-wide sub-vertical fault zone juxtaposing sheared alluvium and fault Cretaceous basement rocks This section is overlain by a 6-m-thick fluvial terrace. Trenching in the unfaulted terrace immediately overlying the fault trace revealed radiocarbon and OSL ages ranging from 20 to 21 ka that are consistent with a prominent unfaulted alluvial fan along the projection of this fault 1.5 km to

  15. Las parodias dramáticas de Don Juan Tenorio en el siglo XIX

    OpenAIRE

    Noh Kang, Sook-Hwa

    2016-01-01

    Zorrilla was, undoubtedly, the one who approached the myth of Don Juan the closest. Tirso has paternity. Madariaga thinks the Don Juan of Moliere lacks spontaneity and displays a conscious rebellion against the laws. That of Byron is a reflective character. The Don Giovanni created by Da Ponte for Mozart's opera is very civilised and lacks spontaneity, as well. Madariaga does not forgive Pushkin for the appearance of Laura on a balcony in Madrid and not in Seville. Obviously Madariaga is righ...

  16. Estrategias de producción teatral : "Don Juan Tenorio" en 1950

    OpenAIRE

    Cornejo Ibares, Mª Paz

    2007-01-01

    Este artículo supone un análisis de las estrategias de producción escénica de los distintos montajes de Don Juan Tenorio de José Zorrilla representados en Madrid durante 1950. This article is an analysis of the scenic production’s strategies in the different staging of Don Juan Tenorio by José Zorrilla performed in Madrid during 1950.

  17. Stenocercus doellojuradoi (Iguanidae, Tropidurinae: una nueva especie para la provincia de San Juan, Argentina

    Directory of Open Access Journals (Sweden)

    Laspiur, Alejandro

    2006-05-01

    Full Text Available República Argentina, Provincia de San Juan, Depto. Valle Fértil, 3 km al norte de la localidad de Las Tumanas sobre la Ruta Provincial 510 (30°52’ S, 67°20’ W. COLECTOR: Alejandro Laspiur. FECHA: 25 /02/ 2006. MATERIAL DE REFERENCIA: Instituto y Museo de Ciencias Naturales, Universidad Nacional de San Juan: IMCNUNSJ 3000. Un ejemplar macho (LHC: 54 mm..

  18. Juan L. Ortíz: Primeros diálogos

    Directory of Open Access Journals (Sweden)

    Roxana Haydée Páez

    1996-11-01

    Full Text Available Emergencia, contextualización y diferencia de la poética de Juan L. Ortiz. El intertexto de lo que Julia Kristeva considera la primera vanguardia o vanguardia de fin de siglo, de los poetas argentinos posmodernistas, de Juan Ramón Jiménez. Vinculaciones del impresionismo de Ortiz, a través del simbolismo, el modernismo y el sencillismo

  19. Datos biográficos del sr. dr. juan de dios carrasquilla l. 1833-1908

    OpenAIRE

    Revista de la, Facultad de Medicina

    2012-01-01

    Nació el Dr. Juan de Dios Carrasquilla Lema en la ciudad deBogotá, el día 19 de Marzo de 1833, en la casa marcada hoy con el número 210 de la carrera 8, antigua Calle de Florián.  Fueron sus padres D. Juan Manuel Carrasquilla y D. Candelaria Lema, naturales de Medellín.

  20. Juan L. Ortíz: Primeros diálogos

    Directory of Open Access Journals (Sweden)

    Roxana Haydée Páez

    1996-11-01

    Full Text Available Emergencia, contextualización y diferencia de la poética de Juan L. Ortiz. El intertexto de lo que Julia Kristeva considera la primera vanguardia o vanguardia de fin de siglo, de los poetas argentinos posmodernistas, de Juan Ramón Jiménez. Vinculaciones del impresionismo de Ortiz, a través del simbolismo, el modernismo y el sencillismo

  1. THE SONG OF SONGS IN THE WORK OF LUIS DE LEON, SAN JUAN DE LA CRUZ Y JUAN GELMAN LANGUAGE, CHILDREN AND SPIRITUAL EXPERIENCE

    Directory of Open Access Journals (Sweden)

    Geneviève Fabry

    2015-04-01

    Full Text Available This paper aims to study the intertextual traces dell Song of Songs in the work of Luis de León, San Juan de la Cruz and Juan Gelman from a particular point of view: the resonance in the vernacular of small biblical book as founder dimension of an experience spiritual, emotional and literary time. Choosing our corpus may surprise but, for one, would like to emphasize the importance of two pivotal moments in the reception of the Song: the Spanish Renaissance, whose approach to the Bible is enriched by new currents philological and exegetical of which Luis de León is a sublime representative; the late twentieth century in which an insistent intertextuality observed with sanjuanista work in one direction often foreign to Christian spirituality, as in the case of the Argentine Juan Gelman.

  2. Hydrologic data for the San Juan and Animas River valleys in the Farmington, Aztec, Bloomfield, and Cedar Hill areas, San Juan County, New Mexico

    Science.gov (United States)

    McAda, D.P.; Shelton, S.G.

    1987-01-01

    In July 1985, the U.S. Geological Survey initiated a three-year study in San Juan County, New Mexico, to determine the concentrations of chemical constituents in the groundwater in the San Juan and Animas River valleys and to determine the direction and rate of groundwater flow and its relation to river stage. The study was conducted in cooperation with the San Juan County Commission and the New Mexico Oil Conservation Division. The data that was collected during the first 1-1/2 yr of the study is completed. The report includes well records for 51 wells and water levels from 23 wells, hydrographs from four observation wells and one river stage site, and available chemical analyses from 50 wells and 14 surface water sites. Water samples from six wells and one surface-water site were analyzed for purgeable organic chemicals; none were detected. (Lantz-PTT)

  3. A new record for American Bullfrog (Lithobates catesbeianus in San Juan, Argentina Nuevo registro de rana toro americana (Lithobates catesbeianus en San Juan, Argentina

    Directory of Open Access Journals (Sweden)

    Eduardo Sanabria

    2011-03-01

    Full Text Available We report a new record of Lithobates catesbeianus (American bullfrog from Argentina. L. catesbeianus was first introduced to San Juan Province 11 years ago in Calingasta Department, where the habitat is pre-cordilleran. The new record is for Zonda Department, San Juan Province, in the Monte desert region. Here, L. catesbeianus uses artificial ponds for reproduction and tadpole development. These ponds receive water from an irrigation system that connects the whole agriculture land in the region. The tadpoles use the irrigation canals to move among ponds. We suggest that legislation should be established to prevent future invasions and to achieve sustainable management of the wild American bullfrog populations in San Juan. Prevention of future invasion and management of established populations of this species requires the cooperation of numerous stake holders.Se presenta un nuevo registro de Lithobates catesbeianus (rana toro americana en Argentina. L. catesbeianus fue introducida por primera vez a la provincia de San Juan hace 11 años en el Departamento Calingasta, donde el hábitat es pre-cordillerano. El nuevo registro es para el Departamento Zonda en la provincia de San Juan, en el desierto del Monte. En este sitio, L. catesbeianus usa estanques artificiales para la reproducción y desarrollo del renacuajo. Los estanques reciben agua de un sistema de riego que conecta todas las tierras de la agricultura en la región. Los renacuajos utilizan los canales de riego para moverse entre los estanques. Sugerimos que se establezcan leyes para prevenir invasiones futuras y para lograr un manejo integrado de las poblaciones silvestres de rana toro que se encuentran en San Juan. La prevención de futuras invasiones y el manejo de las poblaciones establecidas de esta especie requieren la cooperación de numerosas entidades tanto gubernamentales como privadas.

  4. Juan Gelman: Fábulas y revolución poética = Juan Gelman: Fables and poetic revolution

    Directory of Open Access Journals (Sweden)

    Lin, Ching-Yu

    2016-09-01

    Full Text Available Resumen: El presente estudio se centra en Fábulas de Juan Gelman para analizar la vinculación entre el género fabulístico y la revolución poética subyacente en su obra. Se trata de un nuevo modo de contar la realidad y las circunstancias sociales. Los poemas mezclan la historia oficial y la ficción para proponer miradas alternativas y subvertir convencionalismos y formalismos. Además, se crean personajes anónimos a fin de relatar visiones fabulosas en relación con la identidad colectiva. Esta técnica causa un efecto del extrañamiento y distanciamiento que desemboca en una reflexión profunda sobre la vida real. Finalmente, se reescriben leyendas exóticas, inventando episodios, con la finalidad de llamar la atención sobre ciertos fenómenos de la sociedad actual e inspirar solidaridad. = Abstract: This study focuses on Fábulas of Juan Gelman. The aim is to analyze the relationship between fable genre and poetic revolution in this work, in which Gelman provides a new way of describing reality and social circumstances. His poems combine official history with fiction to propose alternative views and subvert conventionalism and formalism. In addition, this poet creates anonymous characters to relate fabulous visions regarding the collective identity. This technique results in estrangement and distancing, which lead to profound reflection on real life. Finally, exotic legends are rewritten by means of invented episodes to draw attention to certain phenomena of modern society and inspire solidarity.

  5. Demistifikasi Tokoh Don Juan dalam La Nuit De Valognes Karya Eric-Emmanuel Schmitt

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    Talha Bachmid

    2006-10-01

    Full Text Available Don Juan is a myth about a seducer, a man with many love adventures, a women’s heat conqueror, and many other predicates. Don Juan as a myth is well-known world wide over, appearing in about 50 writings such as novels, plays, operas. But a French writer Eric-Emannuel Schmitt proposes a new figure of Don Juan in his play La Nuit de Valognes (Night in Valognes: the seducer is judged by five victims and convicted to marry one of his victims in order to stop his adventures. Surprisingly, Don Juan accepts this verdict without conditions. This sudden change becomes the theme of the play: the demythification of Don Juan. A flash back scene shows the beginning of this change and the interpretations that might arise from here. One possible interpretation is proposed and discussed in this paper: Don Juan found the way to a purer love, which is the union with God, and decides to begin his journey. This play can thus be categorised as a sufi text.

  6. Measuring intracellular calcium dynamics of HeLa cells exposed to nitric oxide by microplate fluorescence reader

    Science.gov (United States)

    Huang, Yimei; Chen, Jiangxu; Yang, Hongqin; Zheng, Liqin; Wang, Yuhua; Li, Hui; Xie, Shusen

    2012-12-01

    Nitric oxide (NO) has been reported to have the ability to promote or inhibit the proliferation and metastasis of cancer cells. It appears to have an effect on inducing calcium transient, which participates in essential cellular signaling in the physiological and pathological processes. Our work was intended to study the effects of exogenous NO on intracellular calcium dynamics of HeLa cells with Fluo-3, a calcium fluorescent indicator by microplate fluorescence reader. The results showed that after NO donor was injected into the wells, intracellular Ca2+ fluorescence intensity increased significantly compared with that of control group. Furthermore, the calcium transient activated by NO was mainly due to the calcium release from intracellular calcium stores. These would be helpful to further recognize the role of NO involved in cancer cell proliferation and metastasis.

  7. Neutral red cytotoxicity assays for assessing in vivo carbon nanotube ecotoxicity in mussels--Comparing microscope and microplate methods.

    Science.gov (United States)

    Miller, M A; Bankier, C; Al-Shaeri, M A M; Hartl, M G J

    2015-12-30

    The purpose of the present study was to compare two neutral red retention methods, the more established but very labour-intensive microscope method (NRR) against the more recently developed microplate method (NRU). The intention was to explore whether the sample volume throughput could be increased and potential operator bias avoided. Mussels Mytilus sp. were exposed in vivo to 50, 250 and 500 μg L(-1) single (SWCNTs) or multi-walled carbon nanotubes (MWCNTs). Using the NRR method, SWCNTs and MWCNTs caused concentration dependent decreases in neutral red retention time. However, a concentration dependent decrease in optical density was not observed using the NRU method. We conclude that the NRU method is not sensitive enough to assess carbon nanotube ecotoxicity in vivo in environmentally relevant media, and recommend using the NRR method.

  8. A Sensitive Microplate Assay for Lipase Activity Measurement Using Olive Oil Emulsion Substrate: Modification of the Copper Soap Colorimetric Method.

    Science.gov (United States)

    Mustafa, Ahmad; Karmali, Amin; Abdelmoez, Wael

    2016-01-01

    The present work involves a sensitive high-throughput microtiter plate based colorimetric assay for estimating lipase activity using cupric acetate pyridine reagent (CAPR). In the first approach, three factors two levels factorial design methodology was used to evaluate the interactive effect of different parameters on the sensitivity of the assay method. The optimization study revealed that the optimum CAPR concentration was 7.5% w/v, the optimum solvent was heptane and the optimum CAPR pH was 6. In the second approach, the optimized colorimetric microplate assay was used to measure lipase activity based on enzymatic hydrolysis of olive oil emulsion substrate at 37°C and 150 rpm. The emulsion substrates were formulated by using olive oil, triton X-100 (10% v/v in pH 8) and sodium phosphate buffer of pH 8 in ratio of 1:1:1 in the case of Candida sp. lipase. While in the case of immobilized lipozyme RMIM, The emulsion substrates were formulated by using olive oil, triton X-100 (1% v/v in pH 8) and sodium phosphate buffer of pH 8 in ratio of 2:1:1. Absorbance was measured at 655 nm. The stability of this assay (in terms of colored heptane phase absorbance readings) retained more than 92.5% after 24 h at 4°C compared to the absorbance readings measured at zero time. In comparison with other lipase assay methods, beside the developed sensitivity, the reproducibility and the lower limit of detection (LOD) of the proposed method, it permits analyzing of 96 samples at one time in a 96-well microplate. Furthermore, it consumes small quantities of chemicals and unit operations.

  9. Cellphone-Based Hand-Held Microplate Reader for Point-of-Care Testing of Enzyme-Linked Immunosorbent Assays.

    Science.gov (United States)

    Berg, Brandon; Cortazar, Bingen; Tseng, Derek; Ozkan, Haydar; Feng, Steve; Wei, Qingshan; Chan, Raymond Yan-Lok; Burbano, Jordi; Farooqui, Qamar; Lewinski, Michael; Di Carlo, Dino; Garner, Omai B; Ozcan, Aydogan

    2015-08-25

    Standard microplate based enzyme-linked immunosorbent assays (ELISA) are widely utilized for various nanomedicine, molecular sensing, and disease screening applications, and this multiwell plate batched analysis dramatically reduces diagnosis costs per patient compared to nonbatched or nonstandard tests. However, their use in resource-limited and field-settings is inhibited by the necessity for relatively large and expensive readout instruments. To mitigate this problem, we created a hand-held and cost-effective cellphone-based colorimetric microplate reader, which uses a 3D-printed opto-mechanical attachment to hold and illuminate a 96-well plate using a light-emitting-diode (LED) array. This LED light is transmitted through each well, and is then collected via 96 individual optical fibers. Captured images of this fiber-bundle are transmitted to our servers through a custom-designed app for processing using a machine learning algorithm, yielding diagnostic results, which are delivered to the user within ∼1 min per 96-well plate, and are visualized using the same app. We successfully tested this mobile platform in a clinical microbiology laboratory using FDA-approved mumps IgG, measles IgG, and herpes simplex virus IgG (HSV-1 and HSV-2) ELISA tests using a total of 567 and 571 patient samples for training and blind testing, respectively, and achieved an accuracy of 99.6%, 98.6%, 99.4%, and 99.4% for mumps, measles, HSV-1, and HSV-2 tests, respectively. This cost-effective and hand-held platform could assist health-care professionals to perform high-throughput disease screening or tracking of vaccination campaigns at the point-of-care, even in resource-poor and field-settings. Also, its intrinsic wireless connectivity can serve epidemiological studies, generating spatiotemporal maps of disease prevalence and immunity.

  10. A universally calibrated microplate ferric reducing antioxidant power (FRAP) assay for foods and applications to Manuka honey.

    Science.gov (United States)

    Bolanos de la Torre, Amparo Angelica S; Henderson, Terence; Nigam, Poonam Singh; Owusu-Apenten, Richard K

    2015-05-01

    The ferric reducing antioxidant power (FRAP) assay was recently adapted to a microplate format. However, microplate-based FRAP (mFRAP) assays are affected by sample volume and composition. This work describes a calibration process for mFRAP assays which yields data free of volume effects. From the results, the molar absorptivity (ε) for the mFRAP assay was 141,698 M(-1) cm(-1) for gallic acid, 49,328 M(-1) cm(-1) for ascorbic acid, and 21,606 M(-1) cm(-1) for ammonium ferrous sulphate. The significance of ε (M(-1) cm(-1)) is discussed in relation to mFRAP assay sensitivity, minimum detectable concentration, and the dimensionless FRAP-value. Gallic acid showed 6.6 mol of Fe(2+) equivalents compared to 2.3 mol of Fe(+2) equivalents for ascorbic acid. Application of the mFRAP assay to Manuka honey samples (rated 5+, 10+, 15+, and 18+ Unique Manuka Factor; UMF) showed that FRAP values (0.54-0.76 mmol Fe(2+) per 100g honey) were strongly correlated with UMF ratings (R(2)=0.977) and total phenols content (R(2) = 0.982)whilst the UMF rating was correlated with the total phenols (R(2) = 0.999). In conclusion, mFRAP assay results were successfully standardised to yield data corresponding to 1-cm spectrophotometer which is useful for quality assurance purposes. The antioxidant capacity of Manuka honey was found to be directly related to the UMF rating.

  11. Täppispalkamine / Claudio Fernandez-Araoz

    Index Scriptorium Estoniae

    Fernandez-Araoz, Claudio

    2005-01-01

    10 peamist viga, mis tehakse uute töötajate tööle värbamisel ning vigade võimalikest lahendustest. Kommenteerivad Agu Vaher, Terje Tiiman ja Ille Einmann. Vt. samas: Kas teie firma vajaks uue inimese töölevärbamisel välist abi?; Midagi enamat kui vestlus - struktureeritud intervjuu; Kumba valida - armastusväärne tossike või kurjamist proff?

  12. Täppispalkamine / Claudio Fernandez-Araoz

    Index Scriptorium Estoniae

    Fernandez-Araoz, Claudio

    2005-01-01

    10 peamist viga, mis tehakse uute töötajate tööle värbamisel ning vigade võimalikest lahendustest. Kommenteerivad Agu Vaher, Terje Tiiman ja Ille Einmann. Vt. samas: Kas teie firma vajaks uue inimese töölevärbamisel välist abi?; Midagi enamat kui vestlus - struktureeritud intervjuu; Kumba valida - armastusväärne tossike või kurjamist proff?

  13. Reseña de: Rosario Moreno Soldevila, Juan Martos (eds.. Amor y sexo en la literatura latina

    Directory of Open Access Journals (Sweden)

    Gema Molina Mellado

    2017-02-01

    Full Text Available Rosario Moreno Soldevila, Juan Martos (eds.. Amor y sexo en la literatura latina. Review of: Rosario Moreno Soldevila, Juan Martos (eds.. Amor y sexo en la literatura latina. Anexo II de Exemplaria Classica. Huelva: Servicio de Publicaciones, 2015. 267 págs. ISSN 2173-6839. // Rosario Moreno Soldevila, Juan Martos (eds.. Amor y sexo en la literatura latina // Reseña de: Rosario Moreno Soldevila, Juan Martos (eds.. Amor y sexo en la literatura latina. Anexo II de Exemplaria Classica. Huelva: Servicio de Publicaciones, 2015. 267 págs. ISSN 2173-6839.

  14. Hasta siempre Profesor Manuel Fernández: Palabras en homenaje al ex Decano de la Facultad de Arquitectura y Urbanismo Dr. Arqto. Manuel Fernández Hechenleitner (1946- 2003. / Farewell, Professor Manuel Fernandez: Words in tribute to the former - Dean of the Faculty of Architecture and Planning Dr. Arqto. Manuel Fernández Hechenleitner (1946 - 2003.

    Directory of Open Access Journals (Sweden)

    Javier de Cárdenas

    2003-06-01

    Full Text Available Académicos, ex alumnos y amigos de la Facultad de Arquitectura y Urbanismo de la Universidad de Chile, recuerdan la personalidad del Dr. Arquitecto Manuel Fernández Hechenleitner (1946-2003, ex Decano de la F.A.U., con motivo de su fallecimiento./Academics, ex - students and friends of the School of Architecture and Urbanism of the University of Chile, remember the personality of Dr. Manuel Fernandez Hechenleitner Architect (1946-2003, ex - Dean of the FAU, on the occasion of his death.

  15. 梯形微板法在ABO反定型中的应用%Application of Trapezoidal Microplate Method to Reverse ABO Grouping

    Institute of Scientific and Technical Information of China (English)

    杜雪宁; 刘芳兰; 王振卿; 张来玉

    2011-01-01

    Objective To evaluate the application of trapezoidal microplate method to reverse ABO grouping. Methods Im-munological reaction of blood group antigen and antibody were carried out on trapezoidal microplate, and the blood group was determined by judging the agglutination by using high-tech camera digital analysis. The method was evaluated for accuracy, reliability, reproducibility and resistance to interference of blood lipid, using U-shaped microplate method as control. Results The accuracy and reproducibility of trapezoidal microplate method were 99. 97% and 100% respectively, and the interference of blood lipid was effectively avoided. All the indexed of the method were superior to those of U-shaped microplate method. Conclusion Trapezoidal microplate method is a high accurate and stable method for reverse ABO grouping, which is more suitable for the treatment of a large quantity of samples in blood station system.%目的 评价梯形微板法在ABO反定型中的应用.方法 采用梯形微板进行血型抗原抗体免疫学反应,利用高科技摄像数码分析技术进行凝集判别,确定血型,分析该方法的准确性、可靠性、重复性和脂血抗干扰性,同时以U型微板法作平行对照.结果 梯形微板法的准确性为99.97%,可靠性较高,重复性为100%,且能有效避免脂血的干扰,各项指标均优于U型微板法.结论 梯形微板法在ABO反定型鉴定中是一项准确性高、稳定性好的检测手段,更适合于血站系统的大批量标本的结果处理.

  16. Umbrales: La escritura cinemática de Juan Emar

    Directory of Open Access Journals (Sweden)

    Wolfgang Bongers

    2014-07-01

    Full Text Available Este trabajo desarrollará la idea de que la escritura crítica, metaficcional y autorreferente de Juan Emar -desde las "Notas de Arte" (1924-1927 hasta el proyecto monumental de Umbral (1940-1964- puede comprenderse, por un lado, como espacio de resonancia de una estructura cinemática de percepción que se perfila como fundamental experiencia de vida durante la primera mitad del siglo XX. Por otro lado, sus textos, en diferentes niveles enunciativos, son parte de la conceptualización de esta misma experiencia. En este sentido, lo cinemático es un imaginario multiforme de la percepción del movimiento que culmina en el cine de vanguardia de los años veinte; mientras que lo cinematográfico se relaciona con el fenómeno del cine propiamente tal, sus posibilidades tecnológicas y los elementos que lo convierten en espectáculo. Estos polos tensionan la escritura de Emar en el nivel referencial, reflexivo, y estético-formal.

  17. The Yale/San Juan Southern Proper Motion Program

    Science.gov (United States)

    van Altena, W. F.; Girard, T. M.; Platais, I.; Kozhurina-Platais, V.; Ostheimer, J.; Lopez, C. E.; Mendez, R. A.

    1999-09-01

    The SPM is based on photographic plates taken at our observatory at El Leoncito, Argentina and will yield absolute proper motions and positions to magnitude B 19 for approximately 1 million stars south of declination -20 degrees. The SPM is a joint program between the Yale Southern Observatory and the Universidad Nacional de San Juan, Argentina. The SPM Catalog 2.0 provides positions, absolute proper motions, and photographic BV photometry for over 320,000 stars and galaxies. All objects contained in the SPM 1.0 Catalog (the South Galactic Pole region) are also included in this version. Note that SPM 1.0 has been replaced by SPM 1.1 which has slightly different astrometry (mostly proper motions) due to refinement of the magnitude equation correction in the SPM 2.0 Catalog. The Catalog covers an area of 3700 square degrees in an irregularly bounded band between declinations of -43 and -22 degrees, but excluding fields in the plane of the Milky Way. Stars cover the magnitude range 5 astrom/. Our web-site contains several useful plots showing the sky coverage, error distribution, a quick comparison with the Hipparcos proper motions, etc.

  18. Juan Rulfo y el ensueño del tiempo

    Directory of Open Access Journals (Sweden)

    Juan Manuel Rodríguez

    2003-01-01

    Full Text Available Este ensayo discute la temporalidad en la narrativa de Juan Rulfo a través de los recursos que utiliza para exponer las tramas de sus personajes. La temporalidad del fantasma Rulfiano es determinante en los enigmas que devienen del campo simbólico en el que se desarrolla la obra. Estos personajes plantean fronteras móviles en su actuación pues la muerte les da un carácter ilimitado de acción perdiendo la secuencia histórica del presente-pasado-futuro. Asimismo se plantean algunas relaciones hipotéticas entre el autor y su obra señalando la polisemia resultante del uso de la metáfora. La estructura de la obra y la suspensión de las tramas hacen del lector un co-autor. A través del ensueño Rulfo inventa una manera de leer y escribir la muerte a través de su fantasmagoría.

  19. Juan Carandell Pericay (1893-1937 y Sierra Nevada

    Directory of Open Access Journals (Sweden)

    Antonio López Ontiveros

    2000-01-01

    Full Text Available Juan Carandell Pericay (1893-1937, geólogo y geógrafo ligado a la Institución Libre de Enseñanza, escribió unos trescientos títulos, con una actividad científica e intelectual muy polifacética: geología, geografía física, humana y regional, libros de viaje y excursiones pedagógicas, semiología gráfica, divulgación científica y otras actividades intelectuales y artísticas. Una parte significativa de dichas obras estuvo dedicada a las Béticas, en general, y a Sierra Nevada en particular. Aspectos considerados por Carandell sobre Sierra Nevada son los relativos a su geología, geomorfología y tectónica, la erosión y sus causas físicas y humanas, la geografía humana –con especial importancia para el tema del hábitat– y otra variada gama de temas entre los que merece mención la faceta de las representaciones del paisaje, en todas las cuales demostró no sólo sus sólidos conocimientos geológicos y geográficos, sino incluso una calidad artística más que apreciable.

  20. Juan Valentin: un gelogo que supo resumir la geologa argentina Juan Valentin: a geologist that compiled the geology of Argentina

    Directory of Open Access Journals (Sweden)

    Florencio Gilberto Aceolaza

    2011-09-01

    Full Text Available Juan Valentin fue un destacado gelogo alemn que arrib a nuestra patria en 1894 para integrarse a los equipos que entonces desarrollaban investigaciones desde el Museo de La Plata y el Museo Nacional de Buenos Aires. Su trabajo lo llev, inicialmente, a estudiar las sierras de Buenos Aires y luego tambin lo hizo en Crdoba, San Luis, Salta y Jujuy. Fue grande su actividad, motivo por el cual, se lo incorpor a la Sociedad Cientfica Argentina asumiendo la revisin y compaginacin de los Anales que en ese tiempo editaba la mencionada institucin. Esta actividad ms el conocimiento de campo logrado, lo llev a confeccionar un extenso artculo donde describi la geologa de la Argentina, el cual fue agregado a la edicin del Segundo Censo Nacional. A este trabajo se lo considera una importante sntesis sobre la constitucin geolgica del pas. Entusiasmado con el apoyo que vena logrando planific un programa de investigacin para desarrollar en el norte de la Patagonia e incrementar el conocimiento de la estratigrafa regional. As fue que, en octubre de en 1897, se dirigi a Puerto Madryn donde llev adelante sus primeros trabajos en el valle del ro Chubut y la zona de Cabo Raso. Revisando afloramientos en las cercanas de Aguada de Reyes muri en un fatal accidente, como dice la crnica, con sus bolsillos repletos de los fsiles que haba coleccionado. Haca poco ms de dos aos que se desempeaba en el pas y contaba con 30 aos recin cumplidos. La mencin histrica narra que su cuerpo fue sepultado en la ciudad de Rawson.Juan Valentin was a German geologist that arrived to Argentina in 1894 to join the staff members of the Museum of La Plata and the National Museum of Buenos Aires. His work initially took him to explore the Buenos Aires ranges, and then those of Crdoba, San Luis, Salta and Jujuy. He joined the Argentine Scientific Society, assuming the edition of the Annals. His activities and the achieved knowledge in the field led him to prepare a lengthy article describing the

  1. The observed analysis on the wave spectra of Hurricane Juan (2003)

    Institute of Scientific and Technical Information of China (English)

    XU Fumin; BUI THI Thuy Duyen; PERRIE Will

    2014-01-01

    Hurricane Juan provides an excellent opportunity to probe into the detailed wave spectral patterns and spectral parameters of a hurricane system, with enough wave spectral observations around Juan’s track in the deep ocean and shallow coastal water. In this study, Hurricane Juan and wave observation stations around Juan’s track are introduced. Variations of wave composition are discussed and analyzed based on time series of one-dimensional frequency spectra, as well as wave steepness around Juan’s track:before, dur-ing, and after Juan’s passing. Wave spectral involvement is studied based on the observed one-dimensional spectra and two-dimensional spectra during the hurricane. The standardization method of the observed wave spectra during Hurricane Juan is discussed, and the standardized spectra show relatively conservative behavior, in spite of the huge variation in wave spectral energy, spectral peak, and peak frequency during this hurricane. Spectral widths’ variation during Hurricane Juan are calculated and analyzed. A two-layer nesting WW3 model simulation is applied to simulate the one-dimensional and two-dimensional wave spectra, in order to examine WW3’s ability in simulating detailed wave structure during Hurricane Juan.

  2. Cartas de Juan Carlos Onetti para Julio Payró =Letters from Juan Carlos Onetti to Julio Payró

    Directory of Open Access Journals (Sweden)

    Lucena, Karina de Castilhos

    2014-01-01

    Full Text Available Este artigo analisa as cartas enviadas pelo escritor uruguaio Juan Carlos Onetti (1909- 1994 ao crítico de arte argentino Julio Payró (1899-1971 entre 1937 e 1955. A análise desses documentos contradiz uma faceta generalizante presente na fortuna crítica do autor, que o descreve como homem solitário e frio. Nos textos aqui estudados, Onetti manifesta seu carinho e admiração por Payró. Da mesma forma, as cartas oferecem dados que contestam a imagem do Onetti descompromissado, já que ele demonstra preocupação com os rumos da política de seu país. A correspondência também revela inseguranças e vaidades que a figura pública fazia questão de manter escondidas. Nesse sentido, este trabalho sugere que investigações a partir de documentos pessoais de escritores podem ampliar fortemente a interpretação de sua obra e às vezes se opor a juízos estabelecidos pelos críticos que não tiveram acesso a esse material.

  3. Precambrian ophiolites of Arabia; a summary of geologic settings, U-Pb geochronology, lead isotope characteristics, and implications for microplate accretion, Kingdom of Saudi Arabia

    Science.gov (United States)

    Pallister, John S.; Stacey, J.S.; Fischer, L.B.; Premo, W.R.

    1988-01-01

    Disrupted ophiolites occur in linear belts as much as 900 km long between micro plates that collided during the late Proterozoic to form the Arabian Shield. The U-Pb zircon ages and lead-isotope data from these ophiolitic rocks help constrain the history of accretion of the Arabian Shield and thereby contribute to the definition of its microplates and terranes. Microplates of the central and western Arabian Shield are generally thought to represent intra-oceanic island arcs that range in age from about 900 Ma to 640 Ma; however, a region of the eastern Arabian Shield contains rocks of early Proterozoic age and may represent an exotic continental fragment entrained between the arc complexes.

  4. 33 CFR 334.1180 - Strait of Juan de Fuca, Wash.; air-to-surface weapon range, restricted area.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Strait of Juan de Fuca, Wash.; air-to-surface weapon range, restricted area. 334.1180 Section 334.1180 Navigation and Navigable... REGULATIONS § 334.1180 Strait of Juan de Fuca, Wash.; air-to-surface weapon range, restricted area. (a) The...

  5. 33 CFR 162.260 - Channel leading to San Juan Harbor, P.R.; use, administration, and navigation.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Channel leading to San Juan Harbor, P.R.; use, administration, and navigation. 162.260 Section 162.260 Navigation and Navigable... WATERWAYS NAVIGATION REGULATIONS § 162.260 Channel leading to San Juan Harbor, P.R.; use, administration...

  6. Friday Harbor Marina Expansion Study--San Juan Island, Washington: Final Detailed Project Report and Environmental Assessment.

    Science.gov (United States)

    1981-04-01

    Juan Marina Inc. Don F. Brown Self Steve F. Brown San Juan Marina Inc. Thoms Chittenden Self Alex D. Crichton Self Alan C,-mings KGMI-Bellingham Lois...Self Susan E. Risser Self Noble W. Starr Self Thomas C. Starr Self Corinne R. Towne Se"I Michael P. Vouri Bellingham Marine Industries June M. Vynne Self

  7. Micro-Photoluminescence (micro-PL) Study of Core Shell GaAs/GaAsSb Nanowires Grown by Self-Assisted Molecular Beam Epitaxy

    Science.gov (United States)

    2015-06-18

    transistor (FET) and light emitting diode (LED) applications. In this thesis, room temperature and low temperature (4K) micro- photoluminescence (micro...public release; distribution is unlimited. Micro- Photoluminescence (micro-PL) Study of Core-Shell GaAs/GaAsSb Nanowires grown by Self-Assisted Molecular...U.S. Army Research Office P.O. Box 12211 Research Triangle Park, NC 27709-2211 GaAsSb, Core Shell Nanowires, Micro Photoluminescence

  8. Development of a pneumatically driven active cover lid for multi-well microplates for use in perfusion three-dimensional cell culture

    OpenAIRE

    Song-Bin Huang; Dean Chou; Yu-Han Chang; Ke-Cing Li; Tzu-Keng Chiu; Yiannis Ventikos; Min-Hsien Wu

    2015-01-01

    Before microfluidic-based cell culture models can be practically utilized for bioassays, there is a need for a transitional cell culture technique that can improve conventional cell culture models. To address this, a hybrid cell culture system integrating an active cover lid and a multi-well microplate was proposed to achieve perfusion 3-D cell culture. In this system, a microfluidic-based pneumatically-driven liquid transport mechanism was integrated into the active cover lid to realize 6-un...

  9. Use of a high-throughput umu-microplate test system for rapid detection of genotoxicity produced by mutagenic carcinogens and airborne particulate matter.

    Science.gov (United States)

    Oda, Yoshimitsu; Funasaka, Kunihiro; Kitano, Masaaki; Nakama, Akihiko; Yoshikura, Taro

    2004-01-01

    In the present study, we developed a rapid umu-microplate test system that uses the nitroreductase- and O-acetyltransferase-overproducing Salmonella typhimurium strain NM3009 and the O-acetyltransferase-overproducing S. typhimurium strain NM2009 to detect genotoxic activity in small volume samples. The assay was used to test the genotoxicity of several standard mutagens and environmental samples. Exponentially growing cultures of NM3009, NM2009, and the parental strain TA1535/pSK1002 were incubated in 96-well microplates with test chemicals both in the presence and in the absence of rat liver S9. The relative beta-galactosidase activities were then determined colorimetrically using either chlorophenol red-beta-D-galactopyranoside (CPRG) or O-nitrophenyl-beta-D-galactopyranoside (ONPG) as a measure of umuC gene induction activity. The sensitivities of NM3009 without S9 mix and NM2009 with S9 mix to nitroarenes and aromatic amines were up to 24- to 75-fold higher than those of the parent strain. Induction of umuC gene expression was detected more readily with CPRG than ONPG. The umu-microplate assay also detected genotoxicity in organic extracts of particulate matter from air samples collected in Osaka City, Japan. The pattern of the responses suggested that the genotoxic activity of the particulate extract was due primarily to nitrated polycyclic aromatic hydrocarbons. Our results indicate that the umu-microplate assay may be a useful way of carrying out rapid screens for genotoxicity in small-volume environmental samples. Copyright 2004 Wiley-Liss, Inc.

  10. 酶标仪自检方法的建立和应用评价%Establishment and Application of a Self-calibrating Approach for Microplate Readers

    Institute of Scientific and Technical Information of China (English)

    余久如; 黎增文; 潘桂红; 鞠萍

    2011-01-01

    Objective To establish a self-calibration method that monitors and corrects multiple performance indexes for microplate readers in a clinical setting. Methods According to the People's Republic of China national metrological verification regulations for ELISA analyzer,the performance status of a microplate reader Multiskan MK3 was checked by a spectrophotometer. Results Fore Multiskan MK3 microplate reader , readout stability (0.001A) ,wavelength error ( - 1. 0 nm ), wavelength repeatability ( 0nm ), absorbance error ( 0. 006A), Absorbance repeatability ( 0. 17%), sensitivity (0. 018L/mg) ,channel difference (0. 005A) and linear (1. 88%). These results were in line with those required by the regulation. Conclusion Clinical laboratories that bad advanced spectrophotometers may be able to perform self-calibration of microplate readers by following the method described above.%目的 建立酶标仪的自检方法,检定酶标仪的各项性能指标.方法 参照并利用分光光度计,对Multiskan MK3酶标仪各项性能指标进行检定.结果 Multiskan MK3酶标仪示值稳定性(0.001A)、波长示值误差(-1.0 nm)、波长重复性(0 nm)、吸光度示值误差(0.006 A)、吸光度重复性(0.17%)、灵敏度(0.018 L/mg)、通道差异(0.005A)及线性(1.88%)均检定合格.结论 具有性能优良分光光度计的临床实验室可进行酶标仪的自检.

  11. The Juan non-LTR retrotransposon in mosquitoes: genomic impact, vertical transmission and indications of recent and widespread activity

    Directory of Open Access Journals (Sweden)

    Tu Zhijian

    2007-07-01

    Full Text Available Abstract Background In contrast to DNA-mediated transposable elements (TEs, retrotransposons, particularly non-long terminal repeat retrotransposons (non-LTRs, are generally considered to have a much lower propensity towards horizontal transfer. Detailed studies on site-specific non-LTR families have demonstrated strict vertical transmission. More studies are needed with non-site-specific non-LTR families to determine whether strict vertical transmission is a phenomenon related to site specificity or a more general characteristic of all non-LTRs. Juan is a Jockey clade non-LTR retrotransposon first discovered in mosquitoes that is widely distributed in the mosquito family Culicidae. Being a non-site specific non-LTR, Juan offers an opportunity to further investigate the hypothesis that non-LTRs are genomic elements that are primarily vertically transmitted. Results Systematic analysis of the ~1.3 Gbp Aedes aegypti (Ae. aegypti genome sequence suggests that Juan-A is the only Juan-type non-LTR in Aedes aegypti. Juan-A is highly reiterated and comprises approximately 3% of the genome. Using minimum cutoffs of 90% length and 70% nucleotide (nt identity, 663 copies were found by BLAST using the published Juan-A sequence as the query. All 663 copies are at least 95% identical to Juan-A, while 378 of these copies are 99% identical to Juan-A, indicating that the Juan-A family has been transposing recently in evolutionary history. Using the 0.34 Kb 5' UTR as the query, over 2000 copies were identified that may contain internal promoters, leading to questions on the genomic impact of Juan-A. Juan sequences were obtained by PCR, library screening, and database searches for 18 mosquito species of six genera including Aedes, Ochlerotatus, Psorophora, Culex, Deinocerites, and Wyeomyia. Comparison of host and Juan phylogenies shows overall congruence with few exceptions. Conclusion Juan-A is a major genomic component in Ae. aegypti and it has been

  12. Successful operation of a cooperative SLR station of China and Argentina in San Juan

    Institute of Scientific and Technical Information of China (English)

    HAN YanBen; E ALONSO; XIANG QingGe; HUANG DongPing; YIN ZhiQiang; LIU WeiDong; E ACTIS; R PODESTA; WANG TanQiang; GUO TangYong; QU Feng; A M PACHECO; AA GONZALEZ

    2008-01-01

    We introduced the observations and researches using a Satellite Laser Ranging (SLR) system with high precision,which was designed and made in China and installed in the Observatory of San Juan Uni-versity,Argentina,the capability of the system and the achievement of the cooperative procedure be-tween China and Argentina.The SLR station in San Juan,set up by China and Argentina,is quite sig-nificant for improving the distribution of SLR stations and enhancing the orbital coverage of the whole earth.Since the SLR system started to work in the Observatory of San Juan University in the beginning of 2006,the operation is rather good,and rich data with high precision have been obtained.Further plan of the cooperation for the near future is also presented.

  13. A jewel in the desert: BHP Billiton's San Juan underground mine

    Energy Technology Data Exchange (ETDEWEB)

    Buchsbaum, L.

    2007-12-15

    The Navajo Nation is America's largest native American tribe by population and acreage, and is blessed with large tracks of good coal deposits. BHP Billiton's New Mexico Coal Co. is the largest in the Navajo regeneration area. The holdings comprise the San Juan underground mine, the La Plata surface mine, now in reclamation, and the expanding Navajo surface mine. The article recounts the recent history of the mines. It stresses the emphasis on sensitivity to and helping to sustain tribal culture, and also on safety. San Juan's longwall system is unique to the nation. It started up as an automated system from the outset. Problems caused by hydrogen sulfide are being tackled. San Juan has a bleederless ventilation system to minimise the risk of spontaneous combustion of methane and the atmospheric conditions in the mine are heavily monitored, especially within the gob areas. 3 photos.

  14. DOCTOR JUAN CLIMACO HERNANDEZ (Tunja,1881-1961

    Directory of Open Access Journals (Sweden)

    Alfonso Vargas Rubiano

    1984-07-01

    Full Text Available

    Hace un tiempo vengo estudiando con fervorosa dedicación la obra pedagógica, sociopolítica, etnológica y cultural de uno de los grandes boyacenses de este siglo, el médico Juan Clímaco Hernández y he encontrado datos muy valiosos de la historia de nuestra educación médica en las postrimerías del pasado siglo y comienzos del actual, que bien valen la pena ser conocidos puesto que las Memorias de Ibáñez terminan en 1884 y el estudio hecho por el Dr. Juan N. Corpas si bien comprende hasta 1922, es muy poco lo que refiere al período que en nuestra historia nacional se conoce como de la Guerra de los 1000 días.


    EL NACIMIENTO Y BACHILLERATO EN TUNJA (1881-1897

    El 22 de noviembre de 1879, en la solemne sesión de clausura de estudios de la recientemente fundada Facultad de Medicina de la Universidad Nacional de los Estados Unidos de Colombia, recibían el grado de médicos los jóvenes Rodolfo Rueda, Manuel J
    Urruchurtu, Rafael Baquero, Eugenio de la Hoz y Miguel Hernández. Este último, nacido en Santa Marta, de 34 años de edad, había sido maestro de escuela superior en el Departamento del Magdalena, y recién graduado se trasladó a la ciudad de Tunja, como médico del Batallón Bolívar y allí ejerció su profesión hasta su muerte en 1911, alternándola con el periodismo y la política; fue elegido Diputado a la Asamblea de Boyacá en el período 1880-82 por el liberalismo de la provincia. Dicen las crónicas de la época que "a la Botica del Negro Hernández acudían todas las clases sociales, sin distinción alguna, a solicitar sus servicios médicos; los pobres sabían muy bien que en él tenían un defensor de sus derechos, listo siempre a aliviar sus dolores y miserias; y algunos contertulios iban en busca de distracción, charla agradable, tresillo tras los frascos y también, en noches especiales una mesa redonda con carpeta verde dejaba que los dados corriesen libremente

  15. JUAN PABLO LLINÁS CUENTAS (1924-2011

    Directory of Open Access Journals (Sweden)

    Alfredo Jácome Roca

    2011-04-01

    Full Text Available

    Guardo en mi biblioteca tres libros de tipo biográfico con dedicatoria del autor, el académico Juan Pablo Llinás Cuentas (1924-2011. Investigó este culto colega en los años ochenta sobre la vida de algunos de nuestros prohombres, como Atanasio Girardot, José Hilario López y José Celestino Mutis, siendo este último libro comentado en mi columna semanal de otrora, la del diario barranquillense El Heraldo. Como todos los que llevan los genes Llinás, sus raíces estaban en Sabanalarga, de donde  era también el ilustre profesor y académico César Augusto Pantoja. Me honró Llinás con su amistad (al igual que su hijo Adolfo, el ortopedista. Edgardo Coronado –escritor e historiador de la segunda ciudad del Atlántico (por tamaño, pero la primera en antigüedad y en abolengos– dijo de él que “su experiencia de 30 años como profesor le dio paciencia, entendimiento y sabiduría, lo que le permitió escribir diez libros sobre temas de historia, ética, medicina y filosofía, que fueron su satisfacción y el camino para encontrarse y encontrar a los demás”. 

    Mi contacto personal con Llinás fue precisamente en la década de los ochenta, cuando en su condición de Coordinador del Programa de Ciencias Básicas de la recién fundada Universidad del Norte, me llamó en varias ocasiones para que dictara mis cursos de fisiología endocrina en este centro académico de excelencia, faro de ciencia y de cultura. Su fuerte eran –por supuesto– las humanidades. De vocablo educado y gentiles maneras, Juan Pablo sabía de medicina, de historia, de filosofía, de artes plásticas y de poesía. En un grupo de amigos de las letras y de las artes en Barranquilla, organizaba reuniones con amigos personales como el poeta Eduardo Carranza, o divagaba sobre el significado imaginario de esculturas enigmáticas del maestro Edgar Negret.

     Fue Fundador, Decano y Profesor de la Facultad de Medicina

  16. Meteorologically Driven Simulations of Dengue Epidemics in San Juan, PR.

    Directory of Open Access Journals (Sweden)

    Cory W Morin

    2015-08-01

    Full Text Available Meteorological factors influence dengue virus ecology by modulating vector mosquito population dynamics, viral replication, and transmission. Dynamic modeling techniques can be used to examine how interactions among meteorological variables, vectors and the dengue virus influence transmission. We developed a dengue fever simulation model by coupling a dynamic simulation model for Aedes aegypti, the primary mosquito vector for dengue, with a basic epidemiological Susceptible-Exposed-Infectious-Recovered (SEIR model. Employing a Monte Carlo approach, we simulated dengue transmission during the period of 2010-2013 in San Juan, PR, where dengue fever is endemic. The results of 9600 simulations using varied model parameters were evaluated by statistical comparison (r2 with surveillance data of dengue cases reported to the Centers for Disease Control and Prevention. To identify the most influential parameters associated with dengue virus transmission for each period the top 1% of best-fit model simulations were retained and compared. Using the top simulations, dengue cases were simulated well for 2010 (r2 = 0.90, p = 0.03, 2011 (r2 = 0.83, p = 0.05, and 2012 (r2 = 0.94, p = 0.01; however, simulations were weaker for 2013 (r2 = 0.25, p = 0.25 and the entire four-year period (r2 = 0.44, p = 0.002. Analysis of parameter values from retained simulations revealed that rain dependent container habitats were more prevalent in best-fitting simulations during the wetter 2010 and 2011 years, while human managed (i.e. manually filled container habitats were more prevalent in best-fitting simulations during the drier 2012 and 2013 years. The simulations further indicate that rainfall strongly modulates the timing of dengue (e.g., epidemics occurred earlier during rainy years while temperature modulates the annual number of dengue fever cases. Our results suggest that meteorological factors have a time-variable influence on dengue transmission relative to other

  17. Prevalence of the Metabolic Syndrome in San Juan, Puerto Rico

    Science.gov (United States)

    Pérez, Cynthia M.; Guzmán, Manuel; Ortiz, Ana P.; Estrella, Mayra; Valle, Yari; Pérez, Naydi; Haddock, Lillian; Suárez, Erick

    2009-01-01

    Objective The metabolic syndrome is associated with a high risk of diabetes and cardiovascular disease, and Hispanics in the United States have higher rates than do other ethnic groups. We assessed the prevalence of the metabolic syndrome and its individual components in Puerto Rican adults. Methods We conducted a cross-sectional study that used a probability cluster design to select a sample of households of the San Juan metropolitan area from 2005 through 2007. A total of 859 persons aged 21–79 years completed a face-to-face interview, blood pressure and waist circumference measurements, and blood sampling. Our primary outcome measure was metabolic syndrome as defined by the updated NCEP-ATP criteria. Results Prevalence of the metabolic syndrome was 43.3%; 45.3% for men and 42.2% for women (P>.05). Prevalence significantly rose with age, from 12.8% among participants aged 21–29 years to 58.2% for participants aged 70–79 years (P<.001). Corresponding increases in the prevalence of the metabolic syndrome in both men and women were also observed; the prevalence peaked in men aged 50–59 years (62.6%) and in women aged 70–79 years (65.2%). Elevated glucose (49.8%) and abdominal obesity (49.0%) were the most common components of the metabolic syndrome, followed by elevated blood pressure (46.1%), reduced high-density lipoprotein cholesterol (46.0%), and elevated triglycerides (31.3%). Substantial variations were found between men and women in the prevalence of individual components. Conclusions Puerto Ricans have a high prevalence of the metabolic syndrome. This health disparity has implications for diabetes and cardiovascular prevention programs. PMID:19157247

  18. Juan Valentin: un gelogo que supo resumir la geologa argentina

    Directory of Open Access Journals (Sweden)

    Florencio Gilberto Aceolaza

    2011-09-01

    Full Text Available Juan Valentin fue un destacado gelogo alemn que arrib a nuestra patria en 1894 para integrarse a los equipos que entonces desarrollaban investigaciones desde el Museo de La Plata y el Museo Nacional de Buenos Aires. Su trabajo lo llev, inicialmente, a estudiar las sierras de Buenos Aires y luego tambin lo hizo en Crdoba, San Luis, Salta y Jujuy. Fue grande su actividad, motivo por el cual, se lo incorpor a la Sociedad Cientfica Argentina asumiendo la revisin y compaginacin de los Anales que en ese tiempo editaba la mencionada institucin. Esta actividad ms el conocimiento de campo logrado, lo llev a confeccionar un extenso artculo donde describi la geologa de la Argentina, el cual fue agregado a la edicin del Segundo Censo Nacional. A este trabajo se lo considera una importante sntesis sobre la constitucin geolgica del pas. Entusiasmado con el apoyo que vena logrando planific un programa de investigacin para desarrollar en el norte de la Patagonia e incrementar el conocimiento de la estratigrafa regional. As fue que, en octubre de en 1897, se dirigi a Puerto Madryn donde llev adelante sus primeros trabajos en el valle del ro Chubut y la zona de Cabo Raso. Revisando afloramientos en las cercanas de Aguada de Reyes muri en un fatal accidente, como dice la crnica, con sus bolsillos repletos de los fsiles que haba coleccionado. Haca poco ms de dos aos que se desempeaba en el pas y contaba con 30 aos recin cumplidos. La mencin histrica narra que su cuerpo fue sepultado en la ciudad de Rawson.

  19. Late Pleistocene and Holocene uplift history of Cyprus: implications for active tectonics along the southern margin of the Anatolian microplate

    Science.gov (United States)

    Harrison, R.W.; Tsiolakis, E.; Stone, B.D.; Lord, A.; McGeehin, J.P.; Mahan, S.A.; Chirico, P.

    2013-01-01

    The nature of the southern margin of the Anatolian microplate during the Neogene is complex, controversial and fundamental in understanding active plate-margin tectonics and natural hazards in the Eastern Mediterranean region. Our investigation provides new insights into the Late Pleistocene uplift history of Cyprus and the Troodos Ophiolite. We provide isotopic (14C) and radiogenic (luminescence) dates of outcropping marine sediments in eastern Cyprus that identify periods of deposition during marine isotope stages (MIS) 3, 4, 5 and 6. Past sea-levels indicated by these deposits are c. 95±25 m higher in elevation than estimates of worldwide eustatic sea-level. An uplift rate of c. 1.8 mm/year and possibly as much as c. 4.1 mm/year in the past c. 26–40 ka is indicated. Holocene marine deposits also occur at elevations higher than those expected for past SL and suggest uplift rates of c. 1.2–2.1 mm/year. MIS-3 marine deposits that crop out in southern and western Cyprus indicate uniform island-wide uplift. We propose a model of tectonic wedging at a plate-bounding restraining bend as a mechanism for Late Pleistocene to Holocene uplift of Cyprus; uplift is accommodated by deformation and seismicity along the margins of the Troodos Ophiolite and re-activation of its low-angle, basal shear zone.

  20. Enhanced Microwave Absorption Properties of Flexible Polymer Composite Based on Hexagonal NiCo2O4 Microplates and PVDF

    Science.gov (United States)

    Luo, Hui; Wang, Xian; Song, Kai; Yang, Jing; Gong, Rongzhou

    2016-08-01

    Hexagonal NiCo2O4 microplates were synthesized via a facile one-pot hydrothermal method and followed by a subsequent annealing process. The complex permittivity and permeability of a NiCo2O4 and polyvinylidene fluoride (PVDF) composite were investigated over 2-18 GHz. The experiment indicated that the good microwave absorption performance of NiCo2O4@PVDF depends on dielectric loss and quarter-wavelength cancellation. Our results show that the absorption frequency bandwidth of reflection loss (RL) less than -20 dB for the NiCo2O4@PVDF composite can be measured over the frequency range of 3-15.5 GHz with an absorbing thickness that varies in the range of 1.25-5 mm. Furthermore, an optimal RL of -44.8 dB was observed at 10.7 GHz with a thickness of 1.75 mm. The loss mechanism is also discussed.

  1. Detection of c-reactive protein based on a magnetic immunoassay by using functional magnetic and fluorescent nanoparticles in microplates.

    Science.gov (United States)

    Yang, S F; Gao, B Z; Tsai, H Y; Fuh, C Bor

    2014-11-07

    We report the preparation and application of biofunctional nanoparticles to detect C-reactive protein (CRP) in magnetic microplates. A CRP model biomarker was used to test the proposed detection method. Biofunctional magnetic nanoparticles, CRP, and biofunctional fluorescent nanoparticles were used in a sandwich nanoparticle immunoassay. The CRP concentrations in the samples were deduced from the reference plot, using the fluorescence intensity of the sandwich nanoparticle immunoassay. When biofunctional nanoparticles were used to detect CRP, the detection limit was 1.0 ng ml(-1) and the linear range was between 1.18 ng ml(-1) and 11.8 μg ml(-1). The results revealed that the method involving biofunctional nanoparticles exhibited a lower detection limit and a wider linear range than those of the enzyme-linked immunosorbent assay (ELISA) and most other methods. For CRP measurements of serum samples, the differences between this method and ELISA in CRP measurements of serum samples were less than 13%. The proposed method can reduce the analysis time to one-third that of ELISA. This method demonstrates the potential to replace ELISA for rapidly detecting biomarkers with a low detection limit and a wide dynamic range.

  2. A rapid and high-throughput microplate spectrophotometric method for field measurement of nitrate in seawater and freshwater.

    Science.gov (United States)

    Wu, Jiapeng; Hong, Yiguo; Guan, Fengjie; Wang, Yan; Tan, Yehui; Yue, Weizhong; Wu, Meilin; Bin, Liying; Wang, Jiaping; Wen, Jiali

    2016-02-01

    The well-known zinc-cadmium reduction method is frequently used for determination of nitrate. However, this method is seldom to be applied on field research of nitrate due to the long time consuming and large sample volume demand. Here, we reported a modified zinc-cadmium reduction method (MZCRM) for measurement of nitrate at natural-abundance level in both seawater and freshwater. The main improvements of MZCRM include using small volume disposable tubes for reaction, a vortex apparatus for shaking to increase reduction rate, and a microplate reader for high-throughput spectrophotometric measurements. Considering salt effect, two salinity sections (5~10 psu and 20~35 psu) were set up for more accurate determination of nitrate in low and high salinity condition respectively. Under optimized experimental conditions, the reduction rates were stabilized on 72% and 63% on the salinity of 5 and 20 psu respectively. The lowest detection limit for nitrate was 0.5 μM and was linear up to 100 μM (RSDs was 4.8%). Environmental samples assay demonstrated that MZCRM was well consistent with conventional zinc-cadmium reduction method. In total, this modified method improved accuracy and efficiency of operations greatly, and would be realized a rapid and high-throughput determination of nitrate in field analysis of nitrate with low cost.

  3. Susceptibility profiles of Nocardia spp. to antimicrobial and antituberculotic agents detected by a microplate Alamar Blue assay

    Science.gov (United States)

    Zhao, Pan; Zhang, Xiujuan; Du, Pengcheng; Li, Guilian; Li, Luxi; Li, Zhenjun

    2017-01-01

    Nocardia species are ubiquitous in natural environments and can cause nocardiosis. Trimethoprim-sulfamethoxazole has long been the monotherapy treatment of choice, but resistance to this treatment has recently emerged. In this study, we used microplate Alamar Blue assays to determine the antimicrobial susceptibility patterns of 65 standard Nocardia isolates, including 28 type strains and 20 clinical Nocardia isolates, to 32 antimicrobial agents, including 13 little studied drugs. Susceptibility to the most commonly used drug, trimethoprim-sulfamethoxazole, was observed in 98% of the isolates. Linezolid, meropenem, and amikacin were also highly effective, with 98%, 95%, and 90% susceptibility, respectively, among the isolates. The isolates showed a high percentage of resistance or nonsusceptibility to isoniazid, rifampicin, and ethambutol. For the remaining antimicrobials, resistance was species-specific among isolates and was observed in traditional drug pattern types. In addition, the antimicrobial susceptibility profiles of a variety of rarely encountered standard Nocardia species are reported, as are the results for rarely reported clinical antibiotics. We also provide a timely update of antimicrobial susceptibility patterns that includes three new drug pattern types. The data from this study provide information on antimicrobial activity against specific Nocardia species and yield important clues for the optimization of species-specific Nocardia therapies. PMID:28252662

  4. A combination turbidity and supernatant microplate assay to rank-order the supersaturation limits of early drug candidates.

    Science.gov (United States)

    Morrison, John S; Nophsker, Michelle J; Haskell, Roy J

    2014-10-01

    A unique opportunity exists at the drug discovery stage to overcome inherently poor solubility by selecting drug candidates with superior supersaturation propensity. Existing supersaturation assays compare either precipitation-resistant or precipitation-inhibiting excipients, or higher-energy polymorphic forms, but not multiple compounds or multiple concentrations. Furthermore, these assays lack sufficient throughput and compound conservation necessary for implementation in the discovery environment. A microplate-based combination turbidity and supernatant concentration assay was therefore developed to determine the extent to which different compounds remain in solution as a function of applied concentration in biorelevant media over a specific period of time. Dimethyl sulfoxide stock solutions at multiple concentrations of four poorly soluble, weak base compounds (Dipyridamole, Ketoconazole, Albendazole, and Cinnarizine) were diluted with pH 6.5 buffer as well as FaSSIF. All samples were monitored for precipitation by turbidity at 600 nm over 1 h and the final supernatant concentrations were measured. The maximum supersaturation ratio was calculated from the supersaturation limit and the equilibrium solubility in each media. Compounds were rank-ordered by supersaturation ratio: Ketoconazole > Dipyridamole > Cinnarizine ∼ Albendazole. These in vitro results correlated well with oral AUC ratios from published in vivo pH effect studies, thereby confirming the validity of this approach. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association.

  5. El anti-don Juan de María de Zayas

    Directory of Open Access Journals (Sweden)

    Trambaioli, Marcella

    2014-12-01

    Full Text Available All critics agree on considering that María de Zayas writes inspired by the anger that she feels for the women condition. Both in her prose and in the only play we know, La traición en la amistad, Zayas censures with irony and wit the don-Juan type who deceives women leaving them without honour. She does it elaborating an intertextual relationship with El burlador de Sevilla. Specifically, in the play she uses three characters: Liseo, who deceives three women, Fenisa, who is a don-Juan female even more exaggerated than Liseo, and don Juan, the less donjuanesco of all. As a matter of fact, Zayas creates an anti-don Juan similar to the one that Ventura de la Vega will realize in the XIX century with his play El hombre de mundo, a parody of Zorrilla’s Don Juan Tenorio; nevertheless it has a clear ethical purpose: to warn women of his age in order to avoid men’s abuses.Los críticos concuerdan en que María de Zayas escribe inspirada por la cólera que le provoca la condición femenina coetánea. Tanto en sus novelas como en la única comedia conservada, La traición en la amistad, Zayas censura con sutil ironía al tipo donjuanesco que se burla de las mujeres dejándolas sin honor, y lo hace entablando un complejo e ingenioso diálogo intertextual con El burlador de Sevilla. De forma especial, en la pieza lo hace a través de tres figuras: Liseo, que engaña a tres mujeres, Fenisa, un don Juan mujer aún más hiperbólico que Liseo, y don Juan, el menos donjuanesco de todos. En definitiva, crea un anti-don Juan parecido al que Ventura de la Vega realizará en el siglo XIX con El hombre de mundo, parodia del Tenorio de Zorrilla, pero con una clara intencionalidad ética: la de aleccionar a las mujeres de su época para que aprendan a esquivar a los hombres que abusan de ellas.

  6. [The medical world of Juan Gil de Zamora's Historia Naturalis (ca. 1275-1296)].

    Science.gov (United States)

    García Ballester, L; Domínguez, A

    1994-01-01

    The article describes the authors and works which were quoted by the Franciscan Juan Gil de Zamora in his Historia naturalis, a scientific encyclopaedia written between c. 1275 and before 1296, probably in the Franciscan monastery of Zamora (Kingdom of Castille). Juan Gil made wide use of the Avicenna's Canon, Gilbertus de Aquila (Anglicus)'s Compendium medicine, and Salernitan medical literature. His work contributed to the diffusion of these medical authors and works throughout the Christian intellectual milieu of late medieval Castille. This diffusion was not without problems.

  7. Juan Mayorga's adaptations of Lope: La dama boba in the 21st century

    Directory of Open Access Journals (Sweden)

    Mónica Molanes

    2012-12-01

    Full Text Available The adaptations made by one of today’s most eminent playwrights, Juan Mayorga, on the texts by Lope de Vega which were represented during the first decade of the 21st century allow us to analyse some of the most relevant aspects in the reception, canonization and theatrical interpretation of 17th century theatre. This study will thus focus on Lope’s La Dama Boba and the adaptation offered by Juan Mayorga for the Compañía Nacional de Teatro Clásico, directed by Helena Pimenta in 2002.

  8. O estudante endiabrado: representação do mito de Dom Juan em espronceda

    OpenAIRE

    Pandolfi, Maira Angélica [UNESP

    2011-01-01

    The present study is a reflection on the representation of the literary myth of Dom Juan in the work El estudiante de Salamanca (1836-1840), by Joseph de Espronceda. It highlights the most significant aspects of their recreation donjuanesca, since the classic elements taken from the founding work of Tirso de Molina to Romantic Titan which characterizes Dom Félix de Montemar. O presente estudo faz uma reflexão sobre a representação do mito literário de Dom Juan na obra El estudiante de Sala...

  9. Late Miocene to Recent formation of the Aure-Moresby fold-thrust belt and foreland basin as a consequence of Woodlark microplate rotation, Papua New Guinea

    Science.gov (United States)

    Ott, Bryan; Mann, Paul

    2015-06-01

    The Aure-Moresby fold-thrust belt and Aure-Moresby foreland basin are located in the eastern Gulf of Papua (GOP), Papua New Guinea (PNG), and formed during late Miocene-Recent as the result of large-scale, counterclockwise rotation of the 355,000 km2 Woodlark microplate. To document the structure, stratigraphy, and age of convergent deformation along the poorly studied, western edge of the rotating Woodlark microplate, we integrate results of 2,538 km of previously unpublished 2-D seismic reflection data with onshore geologic and GPS studies from previous workers. The late Miocene Aure-Moresby fold-thrust belt is a 400 km long, northwest-trending fold-belt exposed onshore in Papua New Guinea that plunges to the southeast, where continuous folds and northeast-dipping thrusts can be imaged in the subsurface for more than 250 km. The arcuate trend of the Aure-Moresby fold-thrust belt along the southwestern coast and offshore areas of the Papuan peninsula parallels the shape of the adjacent, offshore Aure-Moresby foreland basin and the strike of the transpressional segment of the left-lateral Owen-Stanley fault zone (OSFZ) passing along the center of the Papuan peninsula. As the OSFZ becomes more transtensional east of 148°E, folds of the Aure-Moresby fold-thrust belt along southern coast of the peninsula become less prominent, and the adjacent Aure-Moresby foreland basin transitions into an undeformed Cenozoic passive margin setting. These observations of convergent an left-lateral deformation along the Aure-Moresby fold-thrust belt are consistent with: (1) counterclockwise rotation of the Woodlark microplate known from regional GPS studies; (2) coeval opening of the Woodlark basin along its southern edge in the late Miocene; and (3) rapid subduction at the New Britain trench along its northern edge. The kinematics of the rotating Woodlark microplate are driven by slab pull forces acting on the actively subducting northern edge of the microplate.

  10. [Dietary prescriptions for the elites of the kingdom of Navarre in the 16th century: the cases of Juan Rena and Juan de Alarcón].

    Science.gov (United States)

    Serrano Larráyoz, Fernando

    2014-01-01

    The aim of this article is to present and analyse four dietary prescriptions from the 16th century prepared for Juan Rena, a cleric of Venetian origin, and his servant Juan de Alarcón, which are kept at the Archivo General de Navarra. These documents demonstrate the interest of the patients and physicians in dietetics, understood as a group of health and hygienic measures based on the Galenic res naturales and res non naturales. These four prescriptions are closely related to the ad personam or consilia health regimens, which represent a genre of medical literature whose significance in Renaissance Spain has received little attention. The cases studied reveal the high esteem in which the elites held the possession of therapeutic resources adapted to their individual needs, which were compiled and copied for personal use.

  11. Tectonics of the Nazca-Antarctic plate boundary

    Science.gov (United States)

    Anderson-Fontana, Sandra; Larson, Roger L.; Engeln, Joseph F.; Lundgren, Paul; Stein, Seth

    1987-01-01

    A new bathymetric chart of part of the Chile transform system is constructed, based mainly on an R/V Endeavor survey from 100 deg W to its intersection with the East Ridge of the Juan Fernandez microplate. A generally continuous lineated trend can be followed through the entire region, with the transform valley being relatively narrow and well-defined from 109 deg W to approximately 104 deg 30 min W. The fracture zone then widens to the east, with at least two probable en echelon offsets to the south at 104 deg and 102 deg W. Six new strike-slip mechanisms along the Chile Transform and one normal fault mechanism near the northern end of the Chile Rise, inverted together with other plate-motion data from the eastern portion of the boundary, produce a new best-fit Euler pole for the Nazca-Antarctic plate pair, providing tighter constraints on the relative plate motions.

  12. Tectonics of the Nazca-Antarctic plate boundary

    Science.gov (United States)

    Anderson-Fontana, Sandra; Larson, Roger L.; Engeln, Joseph F.; Lundgren, Paul; Stein, Seth

    1987-01-01

    A new bathymetric chart of part of the Chile transform system is constructed, based mainly on an R/V Endeavor survey from 100 deg W to its intersection with the East Ridge of the Juan Fernandez microplate. A generally continuous lineated trend can be followed through the entire region, with the transform valley being relatively narrow and well-defined from 109 deg W to approximately 104 deg 30 min W. The fracture zone then widens to the east, with at least two probable en echelon offsets to the south at 104 deg and 102 deg W. Six new strike-slip mechanisms along the Chile Transform and one normal fault mechanism near the northern end of the Chile Rise, inverted together with other plate-motion data from the eastern portion of the boundary, produce a new best-fit Euler pole for the Nazca-Antarctic plate pair, providing tighter constraints on the relative plate motions.

  13. La "Paráfrasis a Juan" de Nono de Panópolis. Consideraciones literarias

    OpenAIRE

    Villarrubia Medina, Antonio

    2007-01-01

    Este artículo ofrece algunas observaciones generales sobre la Paráfrasis a Juan de Nono de Panópolis, el autor de las Dionisíacas, basadas en el análisis detallado de algunos aspectos literarios de este extenso poema cristiano hexamétrico.

  14. Mediated Debate, Historical Framing, and Public Art: The Juan de Onate Controversy in El Paso

    Science.gov (United States)

    Perez, Frank G.; Ortega, Carlos F.

    2008-01-01

    In 1988, the El Paso, Texas, city council accepted a proposal to build twelve statues of historically important individuals as part of a downtown revitalization initiative. Juan de Onate was selected as the centerpiece statue of the XII Travelers Memorial of the Southwest. The decision to honor the Spanish colonizer triggered a local controversy…

  15. Juan Rulfo and Machado de Assis: Toward an Integrated New Narrative

    Science.gov (United States)

    Wiseman, David P.; Krause, James R.

    2016-01-01

    Despite apparent connections between Brazilian and Spanish American narratives, comparative scholarship has only recently begun to investigate actively these important points of contact. In this study, we introduce a vital crossover between the two traditions, involving Juan Rulfo and Joaquim Maria Machado de Assis. Rulfo's outspoken affinity for…

  16. Juan Linz ja eestlane Aleksander Kesküla / Kaido Jaanson

    Index Scriptorium Estoniae

    Jaanson, Kaido, 1940-

    2002-01-01

    Eestlasest revolutsionääri Aleksander Kesküla elust Hispaanias aastail 1934-1963. Artikli aluseks on A. Kesküla Hispaania-perioodi väheste sõprade, eelkõige tänaseks rahvusvaheliselt tunnustatud sotsioloogi Juan Linz'i meenutused

  17. El cuento de Juan José Arreola: entre el arte y el horror

    OpenAIRE

    Evangelista Ávila, Iram Isaí

    2016-01-01

    La investigación aborda dos temas en la cuentística de Juan José Arreola: el arte y el horror. Arte y horror aparecen simbolizados dentro de sus cuentos como vehículos miméticos que, al interpretarlos, nos dan la posibilidad de acercarnos a una conce

  18. Isopachs of net coal thickness, Fruitland Formation, San Juan Basin, NM and CO (sjbthkg)

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This as a shapefile and coverage showing the isopachs of total net coal in beds greater than 1.2' thick for the Fruitland Formation, San Juan Basin, Colorado and New...

  19. Los derechos humanos a la luz del pensamiento de Juan Duns Scoto

    OpenAIRE

    2012-01-01

    Con este aporte, quiero presentar a usted querido lector y lectora una aproximación a un problema tan cercano a nosotros en Colombia, como es el caso de los Derechos Humanos, iluminando dicha realidad desde un autor franciscano: Juan Duns Scoto, quien es recordado y celebrado este año (2008) en sus 700 años de muerte.

  20. 78 FR 72060 - Chimney Rock National Monument Management Plan; San Juan National Forest; Colorado

    Science.gov (United States)

    2013-12-02

    ...; ] DEPARTMENT OF AGRICULTURE Forest Service Chimney Rock National Monument Management Plan; San Juan National...) to establish management direction for the land and resources within Chimney Rock National Monument... establishing Chimney Rock National Monument (the Monument) requires preparation of a management plan....

  1. Colombia kunstnik Juan Manuel Echavarría Tallinnas / Kadri Karro

    Index Scriptorium Estoniae

    Karro, Kadri

    2011-01-01

    Juan Manuel Echavarría külastab Tallinna 12.-15. aprillini 2011, 13. apr.-l toimub Eesti Kunstiakadeemia Rüütelkonna hoones tema artist talk. Kunstniku holograafiliste trükiste sarjast "Reekviem NN" (2008-2010), mida eksponeeritakse Tallinna 15. graafikatriennaalil Kumu Kunstimuuseumis

  2. Hurricane recovery at Cabezas de San Juan, Puerto Rico, and research opportunities at Conservation Trust Reserves

    Science.gov (United States)

    Peter L. Weaver; Elizabeth Padilla Rodriguez

    2009-01-01

    The Cabezas de San Juan Natural Reserve (El Faro), an exposed peninsular area located in the Subtropical dry forest of northeastern Puerto Rico, was impacted by hurricanes Hugo (1989) and Georges (1998). From 1998 to 2008, a 0.10 ha plot was used to assess forest structure, species composition, and stem growth. During post-hurricane recovery, stem density, tree height...

  3. JUAN JOSÉ CABALLERO RODRÍGUEZ [Material gráfico

    OpenAIRE

    1980-01-01

    JUAN JOSÉ CABALLERO, TALLISTA DE MADERA DE SANTA MARÍA DE GUÍA, DURANTE LA CELEBRACIÓN DE UNA FERIA REGIONAL. DESTACA COMO UN ARTISTA DE LA TALLA, DESARROLLANDO UNA LABOR EXTRAORDINARIA E INCLUSO EN MEDIOS DOCENTES Copia digital. Madrid: Ministerio de Educación, Cultura y Deporte. Subdirección General de Coordinación Bibliotecaria, 2015

  4. Juan Linz ja eestlane Aleksander Kesküla / Kaido Jaanson

    Index Scriptorium Estoniae

    Jaanson, Kaido, 1940-

    2002-01-01

    Eestlasest revolutsionääri Aleksander Kesküla elust Hispaanias aastail 1934-1963. Artikli aluseks on A. Kesküla Hispaania-perioodi väheste sõprade, eelkõige tänaseks rahvusvaheliselt tunnustatud sotsioloogi Juan Linz'i meenutused

  5. Primer registro de Aplectana hylambatis (Nematoda, Cosmocercidae para anfibios de la provincia de San Juan

    Directory of Open Access Journals (Sweden)

    Quiroga, Lorena B.

    2012-11-01

    Full Text Available La presente nota representa los primeros registros de parásitos (nematodos para anfibios de la provincia de San Juan. Además, se discute la variación morfológica de los nematodos encontrados con otras poblaciones de Argentina.

  6. Los hombres. Juan Ramón Jiménez y Jaime Robledo Uribe

    Directory of Open Access Journals (Sweden)

    Boletín Cultural y Bibliográfico Banco de la República

    1958-05-01

    Full Text Available En el documento se realiza sendas notas de conmemoración  del fallecimiento de don Juan Ramón Jiménez  y don Jaime Robledo Uribe. Esta pequeña nota tiene como fin realizar un homenaje a tan reputados miembros del círculo cultural del país.

  7. Alegoría de la retórica en un biombo de Juan Correa

    Directory of Open Access Journals (Sweden)

    Arnulfo Herrera

    2014-03-01

    Full Text Available A partir de un análisis iconográfico se decodifica una alegoría de la retórica incluida en el Biombo de los cuatro elementos y las artes liberales, pintado en México hacia el año 1700 por Juan Correa.

  8. Reef Fish Inventory of Juan De Nova's Natural Park (Western Indian ...

    African Journals Online (AJOL)

    This paper constitutes the first study on reef fish communities at Juan de Nova, one of ... military base, represent sites which experience minimal direct human influence. ... the resilience of coral reef communities to environmental disturbances.. Keywords: reef fishes, diversity, Eparses Islands, natural reserve, western Indian ...

  9. Juan Rulfo and Machado de Assis: Toward an Integrated New Narrative

    Science.gov (United States)

    Wiseman, David P.; Krause, James R.

    2016-01-01

    Despite apparent connections between Brazilian and Spanish American narratives, comparative scholarship has only recently begun to investigate actively these important points of contact. In this study, we introduce a vital crossover between the two traditions, involving Juan Rulfo and Joaquim Maria Machado de Assis. Rulfo's outspoken affinity for…

  10. Colombia kunstnik Juan Manuel Echavarría Tallinnas / Kadri Karro

    Index Scriptorium Estoniae

    Karro, Kadri

    2011-01-01

    Juan Manuel Echavarría külastab Tallinna 12.-15. aprillini 2011, 13. apr.-l toimub Eesti Kunstiakadeemia Rüütelkonna hoones tema artist talk. Kunstniku holograafiliste trükiste sarjast "Reekviem NN" (2008-2010), mida eksponeeritakse Tallinna 15. graafikatriennaalil Kumu Kunstimuuseumis

  11. Hazard assessment of inorganics to endangered fish in the San Juan River, New Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Hamilton, S.J.; Buhl, K.J. [National Biological Service, Yankton, SD (United States)

    1995-12-31

    Acute toxicity tests were conducted with larval Colorado squawfish (Ptychocheilus lucius) and razorback sucker (Xyrauchen texanus) in a constituted water quality simulating the San Juan River near Shiprock, New Mexico. Tests were conducted with arsenate, copper, selenate, selenite, zinc, and five mixtures of seven to nine inorganics simulating environmental mixtures reported for sites along the San Juan River (Ojo Amarillo Canyon, Gallegos Canyon, Hogback East Drain, Mancos River, and McElmo Creek). Razorback suckers were significantly more sensitive to arsenate, selenate, selenite, Hogback East Drain mixture, and Ojo Amarillo Canyon mixture than were Colorado squawfish. The Gallegos Canyon mixture had greater than additive, i.e., synergistic, toxicity to both species, the Ojo Amarillo Canyon mixture had less than additive, i.e., antagonistic, toxicity to both species, and the Mancos River and McElmo Creek mixtures had additive toxicity to both species. The Hogback East Drain mixture had additive toxicity to Colorado squawfish, but synergistic toxicity to razorback suckers. The major toxic component in the five mixtures was copper. Comparison of 96-hour LC50 values with a limited number of environmental water concentrations from the San Juan River revealed high hazard ratios for copper and all five environmental mixtures. The high hazard ratios suggest inorganic contaminants could adversely affect larval Colorado squawfish and razorback suckers in the San Juan River at sites receiving elevated inorganics.

  12. Comments on the Hurricane Juan report to the Nova Scotia Utility and Review Board

    Energy Technology Data Exchange (ETDEWEB)

    Tedesco, R.R.

    2004-05-14

    Nova Scotia Power Inc. (NSPI) has responded to several recommendations made by an independent consultant regarding its emergency response to Hurricane Juan which hit the Halifax Regional Municipality on September 28, 2003. NSPI is in agreement with most of the recommendations and is enhancing its preparedness for use in disaster scenarios similar to Hurricane Juan. Several recommendations have already been implemented by the power company including, improvements to call centre technology and processes, and better coordination with the electricity market operator and municipal responders. Lessons learned from Hurricane Juan were applied during a snow storm in February 2004, particularly in terms of communications. NSPI strongly disagrees with the consultant's recommendation to bring in hundreds of line crews from outside the region in advance of a major storm. NSPI argues that doing so, would have tripled or quadrupled Hurricane Juan costs to $40 or $50 million with little or no improvement on restoration times. Nova Scotia Power Inc. is a wholly-owned subsidiary of Emera (EMA-TSX). The utility provides more than 97 per cent of electric generation, transmission, and distribution to more than 460,000 customers across Nova Scotia. NSPI is committed to supporting Nova Scotian communities by providing safe, reliable power.

  13. 78 FR 35801 - Safety and Security Zones, San Juan Captain of the Port Zone

    Science.gov (United States)

    2013-06-14

    ... departing from Port of San Juan, Puerto Rico. The safety zone remains in effect until the cruise ship is... arrivals and departures of vessels via a broadcast notice to mariners. (c) Definitions. (1) Cruise ship. For the purposes of this section, Cruise ship means a passenger vessel greater than 100 feet in...

  14. Microplate chemiluminescence enzyme immunoassay for the quantitative evaluation of carbohydrate antigen 72-4 in human serum

    Institute of Scientific and Technical Information of China (English)

    JIN Hui; WANG Xu; XIN TianBing; GAO Peng; LIN JinMing; LIANG ShuXuan

    2008-01-01

    A highly sensitive and specific microplate chemiluminescence enzyme immunoassay (CLEIA) was de-veloped for the quantitative evaluation of carbohydrate antigen 72-4 (CA72-4) in human serum, using luminol-H2O2 catalyzed by horseradish peroxidase (HRP) as the chemiluminescence system. The sim-ple and quick determination was accomplished through a sandwich reaction mode. Several physico-chemical parameters of the immunoreaction, including incubation conditions, antibody coating condi-tions, dilution ratio of anti-CA72-4-HRP conjugate, and chemiluminescence reaction time, were studied and optimized. The proposed method exhibited a linear range of 0-200 U/mL with correlation coeffi-cient and detection limit of 0.9995 and 0.18 U/mL, respectively. The inter-assay and intra-assay coeffi-cients of variation (CV) were both less than 10%. The average recovery of two clinical sera with low and high concentration CA72-4 was 99.3% and 98.7%, respectively. Normal tumor markers, including AFP, CEA, CA2.4-2, CA19-9 and CA15-3, did not cross-react with each other. The method's stability was evaluated by assessing its analytical performance after storing the immunoreagents at 4℃ and 37℃ for 7 days. Little difference was found, indicating satisfactory stability of the method. The present method has been successfully applied to the detection of CA72-4 human serum, and showed a good correlation with the commercially available ELISA kit (r2=0.9383). This method showed great potential in the fabrication of diagnostic kit for CA72-4, and could be well used in diagnosis of cancer in clinical practice.

  15. A fluorescent microplate assay quantifies bacterial efflux and demonstrates two distinct compound binding sites in AcrB.

    Science.gov (United States)

    Iyer, Ramkumar; Ferrari, Annette; Rijnbrand, R; Erwin, Alice L

    2015-04-01

    A direct assay of efflux by Escherichia coli AcrAB-TolC and related multidrug pumps would have great value in discovery of new Gram-negative antibiotics. The current understanding of how efflux is affected by the chemical structure and physical properties of molecules is extremely limited, derived from antibacterial data for compounds that inhibit growth of wild-type E. coli. We adapted a previously described fluorescent efflux assay to a 96-well microplate format that measured the ability of test compounds to compete for efflux with Nile Red (an environment-sensitive fluor), independent of antibacterial activity. We show that Nile Red and the lipid-sensitive probe DiBAC4-(3) [bis-(1,3-dibutylbarbituric acid)-trimethine oxonol] can quantify efflux competition in E. coli. We extend the previous findings that the tetracyclines compete with Nile Red and show that DiBAC4-(3) competes with macrolides. The extent of the competition shows a modest correlation with the effect of the acrB deletion on MICs within the compound sets for both dyes. Crystallographic studies identified at least two substrate binding sites in AcrB, the proximal and distal pockets. High-molecular-mass substrates bound the proximal pocket, while low-mass substrates occupied the distal pocket. As DiBAC4-(3) competes with macrolides but not with Nile Red, we propose that DiBAC4-(3) binds the proximal pocket and Nile Red likely binds the distal site. In conclusion, competition with fluorescent probes can be used to study the efflux process for diverse chemical structures and may provide information as to the site of binding and, in some cases, enable rank-ordering a series of related compounds by efflux.

  16. Evaluation of an automated microplate technique in the Galileo system for ABO and Rh(D) blood grouping.

    Science.gov (United States)

    Xu, Weiyi; Wan, Feng; Lou, Yufeng; Jin, Jiali; Mao, Weilin

    2014-01-01

    A number of automated devices for pretransfusion testing have recently become available. This study evaluated the Immucor Galileo System, a fully automated device based on the microplate hemagglutination technique for ABO/Rh (D) determinations. Routine ABO/Rh typing tests were performed on 13,045 samples using the Immucor automated instruments. Manual tube method was used to resolve ABO forward and reverse grouping discrepancies. D-negative test results were investigated and confirmed manually by the indirect antiglobulin test (IAT). The system rejected 70 tests for sample inadequacy. 87 samples were read as "No-type-determined" due to forward and reverse grouping discrepancies. 25 tests gave these results because of sample hemolysis. After further tests, we found 34 tests were caused by weakened RBC antibodies, 5 tests were attributable to weak A and/or B antigens, 4 tests were due to mixed-field reactions, and 8 tests had high titer cold agglutinin with blood qualifications which react only at temperatures below 34 degrees C. In the remaining 11 cases, irregular RBC antibodies were identified in 9 samples (seven anti-M and two anti-P) and two subgroups were identified in 2 samples (one A1 and one A2) by a reference laboratory. As for D typing, 2 weak D+ samples missed by automated systems gave negative results, but weak-positive reactions were observed in the IAT. The Immucor Galileo System is reliable and suited for ABO and D blood groups, some reasons may cause a discrepancy in ABO/D typing using a fully automated system. It is suggested that standardization of sample collection may improve the performance of the fully automated system.

  17. Geochemistry of two contrasting deep fluids in the Sardinia microplate (western Mediterranean): Relationships with tectonics and heat sources

    Science.gov (United States)

    Paternoster, M.; Oggiano, G.; Sinisi, R.; Caracausi, A.; Mongelli, G.

    2017-04-01

    The Sardinia microplate in the western Mediterranean represents an ideal example for examining the relationship between fluid geochemistry, tectonic and heat sources in hydrothermal circuits. It consists of a portion of Variscan basement partly covered by sedimentary (mainly carbonate) and volcanic successions that record significant Permian to Pliocene geodynamic events within the southern European margin. The regional structure of the northern Sardinia is dominated by Tertiary ENE-WSW trending strike-slip and NNW-SSE trending normal faults, both capable of controlling deep and shallow fluid circulation. In this paper, results of a detailed geochemical investigation of waters and gases coming from a W-E trending section of central-north Sardinia are presented in order to explain the contrasting thermal and geochemical features of two - already known groups - of fluids. The Volcanic Logudoro Waters (VLW) is a group of cold to hypothermal Na-HCO3 waters characterised by high CO2 contents and mantle-derived He, that are localized in the volcanic-dominated Tertiary grabens. The He mantle signature within the VLW waters is associated with Plio-Pleistocene Quaternary volcanism where the outgassing of mantle-derived fluids is reasonably due recently active magma sources at depth. The currently active emission of mantle-derived gas linked to cold and hypothermal waters, provides evidence that the heat diffusion associated with the Plio-Pleistocene volcanism has already ended. In contrast, the Granite Variscan Basement Waters (GBW) group is characterised by hot-NaCl-rich waters, containing high concentrations of both dissolved N2 and radiogenic 4He. The high contribution of 4He produced by radiogenic decay of U and Th in the crust indicates a supply of radiogenic heat to the hydrothermal system localized within the granitic basement or in the tectonic contact between granite and Tertiary covers.

  18. Juan Moctezuma y Cortés: cacique, cura e insurgente (1754/57-1816)1/Juan Moctezuma and Cortés: Cacique, Priest and Insurgent (1754/57-1816)

    National Research Council Canada - National Science Library

    Patricia Cruz Pazos

    2011-01-01

    This paper analyses Juan Moctezuma and Cortés' figure, one of the four hundred insurgent priests that lived in New Spain, in order to tackle his origin and vital experience during the last years of colonial period...

  19. Cultura y espiritualidad en la edad moderna valenciana: Juan de Juanes y el ostensorio bifaz de la iglesia parroquial de San Andrés de l’Alcúdia

    Directory of Open Access Journals (Sweden)

    Franco Llopis, Borja

    2009-09-01

    Full Text Available Taking as a starting point the paintings of Juan de Juanes for the church of l’Alcúdia, the author analyses the social questions that might have had a bearing on his artistic production, iconography and diffusion in the Valencian sphere. The possible influence of Erasmus, intimist philosophy, the Christology of Eiximenis and the indoctrination of the Moriscos are some of the aspects discussed.

    En este artículo, partiendo de las pinturas de Juan de Juanes en l’Alcúdia, analizamos las cuestiones sociales que pudieron incidir en su producción artística, iconografía y difusión dentro del ámbito valenciano. La posible influencia del erasmismo, la filosofía intimista, la cristología de Eiximenis o el adoctrinamiento a los moriscos son algunos aspectos sobre los que centramos nuestra reflexión.

  20. Comparative cytotoxic and genotoxic potential of 13 drinking water disinfection by-products using a microplate-based cytotoxicity assay and a developed SOS/umu assay.

    Science.gov (United States)

    Zhang, Shao-Hui; Miao, Dong-Yue; Tan, Li; Liu, Ai-Lin; Lu, Wen-Qing

    2016-01-01

    The implications of disinfection by-products (DBPs) present in drinking water are of public health concern because of their potential mutagenic, carcinogenic and other toxic effects on humans. In this study, we selected 13 main DBPs found in drinking water to quantitatively analyse their cytotoxicity and genotoxicity using a microplate-based cytotoxicity assay and a developed SOS/umu assay in Salmonella typhimurium TA1535/pSK1002. With the developed SOS/umu test, eight DBPs: 3-chloro-4-(dichloromethyl)-5-hydroxy-2[5H]-fura3-chloro-4-(dichloromethyl)-5-hydroxy-2-[5H]-furanone (MX), dibromoacetonitrile (DBN), iodoacetic acid (IA), bromochloroacetonitrile (BCN), bromoacetic acid (BA), trichloroacetonitrile (TCN), dibromoacetic acid (DBA) and dichloroacetic acid (DCA) were significantly genotoxic to S. typhimurium. Three DBPs: chloroacetic acid (CA), trichloroacetic acid (TCA) and dichloroacetonitrile (DCN) were weakly genotoxic, whereas the remaining DBPs: chloroacetonitrile (CN) and chloral hydrate (CH) were negative. The rank order in decreasing genotoxicity was as follows: MX > DBN > IA > BCN > BA > TCN > DBA > DCA > CA, TCA, DCN > CN, CH. MX was approximately 370 000 times more genotoxic than DCA. In the microplate-based cytotoxicity assay, cytotoxic potencies of the 13 DBPs were compared and ranked in decreasing order as follows: MX > IA > DBN > BCN > BA > TCN > DCN > CA > DCA > DBA > CN > TCA > CH. MX was approximately 19 200 times more cytotoxic than CH. A statistically significant correlation was found between cytotoxicity and genotoxicity of the 13 DBPs in S. typhimurium. Results suggest that microplate-based cytotoxicity assay and the developed SOS/umu assay are feasible tools for analysing the cytotoxicity and genotoxicity of DBPs, particularly for comparing their toxic intensities quantitatively.

  1. Development, Optimization, and Validation of a Microplate Bioassay for Relative Potency Determination of Linezolid Using a Design Space Concept, and its Measurement Uncertainty.

    Science.gov (United States)

    Saviano, Alessandro Morais; Francisco, Fabiane Lacerda; Ostronoff, Celina Silva; Lourenço, Felipe Rebello

    2015-01-01

    The aim of this study was to develop, optimize, and validate a microplate bioassay for relative potency determination of linezolid in pharmaceutical samples using quality-by-design and design space approaches. In addition, a procedure is described for estimating relative potency uncertainty based on microbiological response variability. The influence of culture media composition was studied using a factorial design and a central composite design was adopted to study the influence of inoculum proportion and triphenyltetrazolium chloride in microbial growth. The microplate bioassay was optimized regarding the responses of low, medium, and high doses of linezolid, negative and positive controls, and the slope, intercept, and correlation coefficient of dose-response curves. According to optimization results, design space ranges were established using: (a) low (1.0 μg/mL), medium (2.0 μg/mL), and high (4.0 μg/mL) doses of pharmaceutical samples and linezolid chemical reference substance; (b) Staphylococcus aureus ATCC 653 in an inoculum proportion of 10%; (c) antibiotic No. 3 culture medium pH 7.0±0.1; (d) 6 h incubation at 37.0±0.1ºC; and (e) addition of 50 μL of 0.5% (w/v) triphenyltetrazolium chloride solution. The microplate bioassay was linear (r2=0.992), specific, precise (repeatability RSD=2.3% and intermediate precision RSD=4.3%), accurate (mean recovery=101.4%), and robust. The overall measurement uncertainty was reasonable considering the increased variability inherent in microbiological response. Final uncertainty was comparable with those obtained with other microbiological assays, as well as chemical methods.

  2. Validation of a fluorescence polarization assay (FPA) performed in microplates and comparison with other tests used for diagnosing B. melitensis infection in sheep and goats.

    Science.gov (United States)

    Minas, A; Stournara, A; Minas, M; Stack, J; Petridou, E; Christodoulopoulos, G; Krikelis, V

    2007-03-30

    Fluorescence polarization assay (FPA) is a relatively new test for the serological diagnosis of Brucella spp. infection in animals. FPA, carried out in 96-well microplate format, was validated here for diagnosing B. melitensis infection in sheep and goats. This study included sera from 1933 sheep and goats, from animals reared in naturally infected flocks (verified by culture) and showing a positive reaction to two different tests conducted in parallel. In addition, 2154 sera originating from healthy sheep and goats, reared in areas where B. melitensis had never been isolated, were assayed. The optimum cut-off value offering the highest diagnostic sensitivity (DSn) and diagnostic specificity (DSp) was determined at 15 mP over the mean value of the buffer control used in each microplate as determined by receiver operating characteristic analysis. The DSn and DSp of the FPA for small ruminants carried out in microplates at this cut-off value were calculated to be 95.9% and 97.9% with 95% confidence intervals (95% CI) of 94.9-97.7% and 97.2-98.4%, respectively. The accuracy of the FPA, as expressed by determination of the area under the curve, was 0.991. Indirect ELISA and FPA tests offered the highest DSn when compared with the Rose Bengal test, the complement fixation test, the modified Rose Bengal test and competitive ELISA. The parallel or serial combination of FPA with indirect ELISA offers the highest DSn and DSp. As temperature can affect the results of the FPA, all reagents must be at the same temperature and the standard for comparison must always be read under the same conditions as the sera under test. FPA performed in microplates is a promising assay; the DSn and accuracy are better than those of the tests currently approved for diagnosing B. melitensis in small ruminants. Because of its simplicity, speed, and accuracy, this test can improve capacity for laboratory testing and the efficacy of an eradication program based on a test-and-slaughter policy.

  3. Data points (drill locations) used to assess coal resources in the San Juan Basin, CO and NM (sjbptsg)

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This is a shapefile and coverage of data points used in the assessment of coal resources of the Fruitland Formation in the San Juan Basin, Colorado and New Mexico....

  4. Digital Geologic Map of San Juan Island National Historical Park and vicinity, Washington (NPS, GRD, GRE, SAJH, SAJH digital map)

    Data.gov (United States)

    National Park Service, Department of the Interior — The Digital Geologic Map of San Juan Island National Historical Park and vicinity, Washington is composed of GIS data layers complete with ArcMap 9.2 layer (.LYR)...

  5. Influence of host age and host deprivation on longevity,egg load dynamics and oviposition rate in Microplitis rufiventris

    Institute of Scientific and Technical Information of China (English)

    ESMAT MOHAMED HEGAZI; GEHAN MOHAMED ABD EL-AZIZ; AHMED YOUSEF EL-SHAZLY; WEDAD EMAM KHAFAGI

    2007-01-01

    Adult size, longevity, egg load dynamics and oviposition of Microplitis rufiventris Kok. which began their development in the first, second, third (preferred hosts) or fourth (non-preferred hosts) instar larvae of Spodoptera littoralis (Boisd.) were studied. The parasitoid size was largely determined by the initial host size at parasitism. Non-ovipositing females derived from older hosts lived for longer periods than those derived from younger ones. However, the ovipositing females, irrespective of their size, lived for almost the same periods. At emergence, the oviducts of adult females contain a significant amount of mature eggs available for oviposition for a few hours on eclosion day. Egg load increases during the early phase of adult life. The amount of additional mature eggs and rate of egg maturation per hour was greater for wasps derived from preferred hosts compared with those in females derived from non-preferred hosts. The pattern of egg production in M. rufiventris females depended on the availability of hosts for parasitization. Host-deprived females depleted the egg complement with aging; the longer the host deprivation, the lower the oviduct egg load.Marked reduction in both realized or potential fecundity of host-deprived females was observed following host availability. Host privation for more than 3 days induced a marked deficit fecundity pattern through the female's life. The realized fecundity was determined by the interaction among host availability, the number of eggs that are matured over the female's life span, oviposition rate and host size from which the female was derived. These results suggest that: (i) M. rufiventris wasp is a weak synovigenic species; (ii) the maturation of additional eggs is inhibited once the maximum oviduct egg load is reached; (iii) the egg load of the newly emerged female is significantly less than the realized fecundity; and (iv)because M. rufiventris females oviposit fewer eggs when they begin depleting their

  6. On the motion and geometry of the Sierra Nevada Great Valley micro-plate: Implications for Walker Lane tectonics

    Science.gov (United States)

    Kreemer, C.; Hammond, W. C.; Blewitt, G.

    2006-12-01

    The Sierra Nevada Great Valley (SNGV) micro-plate, a.k.a. the Fresno block, has long been recognized as a tectonically stable entity within the Pacific North America plate boundary zone. Some early geodetic studies have confirmed and defined its rigid behavior. However, those studies were based on a very limited amount of geodetic station velocities, and were unable to assess the extent of rigidity towards the edges of the block. The San Andreas and Garlock fault systems define the western and southern edges of the block, but no such features are readily recognizable to the north and east, along the Walker Lane belt. A better assessment of the location of the boundary or transition between the stable SNGV block and the Walker Lane is important for three reasons. It will provide a better understanding of what controls Walker Lane development and evolution, it will provide important boundary conditions in understanding the present-day kinematics of the Walker Lane, and it is contributes to the assessment of seismic hazard levels for the Reno-Carson City area. We analyze data from all the available GPS sites in the greater SNGV region, including data from the SCIGN, BARD and BARGEN networks, semi-continuous data from our own MAGNET network, and campaign-style data (e.g., USGS, SCEC). Also we have started to analyze regional PBO sites, however time-series for most of those sites are at present too short to infer reliable velocity estimates. We use the GIPSY OASIS II software which employs precise point positioning using dual-frequency carrier phase and pseudorange data, and the precise orbit, clock, and reference frame transformation products publicly available from JPL. The analysis includes carrier phase ambiguity resolution and regional filtering. Using these velocities we perform a kinematic analysis of the station velocity solution, solving for an angular velocity that best describes the motion of the SNGV. We analyze the residuals to investigate where the SNGV

  7. Rapid detection of an ABT-737-sensitive primed for death state in cells using microplate-based respirometry.

    Directory of Open Access Journals (Sweden)

    Pascaline Clerc

    Full Text Available Cells that exhibit an absolute dependence on the anti-apoptotic BCL-2 protein for survival are termed "primed for death" and are killed by the BCL-2 antagonist ABT-737. Many cancers exhibit a primed phenotype, including some that are resistant to conventional chemotherapy due to high BCL-2 expression. We show here that 1 stable BCL-2 overexpression alone can induce a primed for death state and 2 that an ABT-737-induced loss of functional cytochrome c from the electron transport chain causes a reduction in maximal respiration that is readily detectable by microplate-based respirometry. Stable BCL-2 overexpression sensitized non-tumorigenic MCF10A mammary epithelial cells to ABT-737-induced caspase-dependent apoptosis. Mitochondria within permeabilized BCL-2 overexpressing cells were selectively vulnerable to ABT-737-induced cytochrome c release compared to those from control-transfected cells, consistent with a primed state. ABT-737 treatment caused a dose-dependent impairment of maximal O(2 consumption in MCF10A BCL-2 overexpressing cells but not in control-transfected cells or in immortalized mouse embryonic fibroblasts lacking both BAX and BAK. This impairment was rescued by delivering exogenous cytochrome c to mitochondria via saponin-mediated plasma membrane permeabilization. An ABT-737-induced reduction in maximal O(2 consumption was also detectable in SP53, JeKo-1, and WEHI-231 B-cell lymphoma cell lines, with sensitivity correlating with BCL-2:MCL-1 ratio and with susceptibility (SP53 and JeKo-1 or resistance (WEHI-231 to ABT-737-induced apoptosis. Multiplexing respirometry assays to ELISA-based determination of cytochrome c redistribution confirmed that respiratory inhibition was associated with cytochrome c release. In summary, cell-based respiration assays were able to rapidly identify a primed for death state in cells with either artificially overexpressed or high endogenous BCL-2. Rapid detection of a primed for death state in

  8. EL CAMINO DEL INCA ENTRE TOCOTA Y VILLA NUEVA (VALLE DE IGLESIA, SAN JUAN / The Inka road between Tocota and Villa Nueva (Iglesia Valley, San Juan

    Directory of Open Access Journals (Sweden)

    Alejandro García

    2011-12-01

    Full Text Available La vialidad estatal incaica en los valles longitudinales de la provincia de San Juan, en el extremo SE del Tawantinsuyu, ha sido objeto de diversas consideraciones durante el último siglo. Sin embargo, no se han realizado estudios específicos destinados a comprobar su existencia, la cual incluso ha sido negada recientemente. A fin de aclarar el tema se realizó el relevamiento de un sector del Valle de Iglesia. En este artículo se presentan los resultados de ese estudio, junto con una revisión y discusión de los antecedentes y de algunas ideas vinculadas con el tema. Fundamentalmente, se verifica la presencia del Qhapaq Ñan en la parte baja del sector analizado, se refuta la propuesta de que el trazado longitudinal principal se habría extendido por la parte alta del sector precordillerano y se sostiene que el tramo relevado fue recorrido por Debenedetti a principios del siglo XX, aunque sin advertir que se trataba del camino incaico.   Palabras clave: Inca; Camino del Inca; Dominación incaica; Collasuyo; San Juan   Abstract The Inka road system in the longitudinal valleys of San Juan province, in the southeastern end of Tawantinsuyu, has been subject of several considerations during the last century. However, no specific studies have been undertaken to verify its existence, which recently has been even denied. To clarify the issue we surveyed a section of Iglesia Valley. This article presents the results of that study, along with a review and discussion of the background and of some ideas related to the topic. Basically, the presence of the Qhapaq Ñan at the bottom of the surveyed sector is verified, and the suggestion that the main inka longitudinal route would have extended over the top of the precordilleran mountain region is refused. Also, it is argued that the analyzed stretch was traveled by Debenedetti in the beginnings of the twentieth century, but without realizing that it was the Inka road.   Keywords: Inka; Inka Road; Inka

  9. Visit of His Excellency Mr Juan Martabit, Ambassador, Permanent Representative of Chile to the United Nations Office in Geneva and other international organisations in Switzerland.

    CERN Multimedia

    Maximilien Brice

    2005-01-01

    Secretary-General; Mrs Juan Martabit. 0502017_07.jpg His Excellency Mr Juan Martabit, Ambassador,Permanent Representative of Chile to the United Nations Office in Geneva and other international organisations in Switzerland visiting the ATLAS building site. From left to right: Prof. Giora Mikenberg, ATLAS Collaboration; Mr Maximilian Metzger, Secretary-General and His Excellency Mr Juan Martabit. 0502017_08.jpg His Excellency Mr Juan Martabit, Ambassador,Permanent Representative of Chile to the United Nations Office in Geneva and other international organisations in Switzerland visiting the ATLAS cavern. From left to right: From left to right: Prof. Giora Mikenberg, ATLAS Collaboration; Mr Maximilian Metzger, Secretary-General; His Excellency Mr Juan Martabit and Mrs Juan Martabit.

  10. Combined microplate-ABTS and HPLC-ABTS analysis of tomato and pepper extracts reveals synergetic and antagonist effects of their lipophilic antioxidative components.

    Science.gov (United States)

    Le Grandois, Julie; Guffond, Delphine; Hamon, Erwann; Marchioni, Eric; Werner, Dalal

    2017-05-15

    The antioxidant capacity of 9 pure lipophilic compounds was examined by microplate-ABTS and HPLC-ABTS, using similar experimental conditions. Results obtained showed that HPLC-ABTS method can be used for a rapid determination of individual antioxidant capacity of compounds in standard solutions or complex mixtures. The application of both methods to real lipophilic extracts from tomato (Solanum lycopersicum L.), green and red peppers (Capsicum annuum) reveals possible interactions between antioxidants. Thus, synthetic mixtures of two compounds identified in tomato and peppers were measured using microplate-ABTS and HPLC-ABTS. Synergistic effects were observed between (β-carotene-capsanthin) (1:9) and (1:1), (α-tocopherol-capsanthin) (1:9), (lutein-lycopene) (9:1) and (capsanthin-δ-tocopherol) (9:1). On the contrary, antagonistic effects were observed for (lutein-δ-tocopherol) and (α-tocopherol-δ-tocopherol). The interactions observed with two-compound mixtures are not systematically observed in the natural lipophilic extracts from tomato, green and red peppers, probably since extracts are more complex and are susceptible to cause interferences.

  11. Size-dependent dynamic pull-in analysis of geometric non-linear micro-plates based on the modified couple stress theory

    Science.gov (United States)

    Askari, Amir R.; Tahani, Masoud

    2017-02-01

    This paper focuses on the size-dependent dynamic pull-in instability in rectangular micro-plates actuated by step-input DC voltage. The present model accounts for the effects of in-plane displacements and their non-classical higher-order boundary conditions, von Kármán geometric non-linearity, non-classical couple stress components and the inherent non-linearity of distributed electrostatic pressure on the micro-plate motion. The governing equations of motion, which are clearly derived using Hamilton's principle, are solved through a novel computationally very efficient Galerkin-based reduced order model (ROM) in which all higher-order non-classical boundary conditions are completely satisfied. The present findings are compared and successfully validated by available results in the literature as well as those obtained by three-dimensional finite element simulations carried out using COMSOL Multyphysics. A detailed parametric study is also conducted to illustrate the effects of in-plane displacements, plate aspect ratio, couple stress components and geometric non-linearity on the dynamic instability threshold of the system.

  12. Development of a cloud point extraction and spectrophotometry-based microplate method for the determination of nitrite in human urine and blood

    Science.gov (United States)

    Zhao, Jiao; Lu, Yunhui; Fan, Chongyang; Wang, Jun; Yang, Yaling

    2015-02-01

    A novel and simple method for the sensitive determination of trace amounts of nitrite in human urine and blood has been developed by combination of cloud point extraction (CPE) and microplate assay. The method is based on the Griess reaction and the reaction product is extracted into nonionic surfactant Triton-X114 using CPE technique. In this study, decolorization treatment of urine and blood was applied to overcome the interference of matrix and enhance the sensitivity of nitrite detection. Multi-sample can be simultaneously detected thanks to a 96-well microplate technique. The effects of different operating parameters such as type of decolorizing agent, concentration of surfactant (Triton X-114), addition of (NH4)2SO4, extraction temperature and time, interfering elements were studied and optimum conditions were obtained. Under the optimum conditions, a linear calibration graph was obtained in the range of 10-400 ng mL-1 of nitrite with limit of detection (LOD) of 2.5 ng mL-1. The relative standard deviation (RSD) for determination of 100 ng mL-1 of nitrite was 2.80%. The proposed method was successfully applied for the determination of nitrite in the urine and blood samples with recoveries of 92.6-101.2%.

  13. Development of a pneumatically driven active cover lid for multi-well microplates for use in perfusion three-dimensional cell culture

    Science.gov (United States)

    Huang, Song-Bin; Chou, Dean; Chang, Yu-Han; Li, Ke-Cing; Chiu, Tzu-Keng; Ventikos, Yiannis; Wu, Min-Hsien

    2015-12-01

    Before microfluidic-based cell culture models can be practically utilized for bioassays, there is a need for a transitional cell culture technique that can improve conventional cell culture models. To address this, a hybrid cell culture system integrating an active cover lid and a multi-well microplate was proposed to achieve perfusion 3-D cell culture. In this system, a microfluidic-based pneumatically-driven liquid transport mechanism was integrated into the active cover lid to realize 6-unit culture medium perfusion. Experimental results revealed that the flow of culture medium could be pneumatically driven in a flow-rate uniform manner. We used the system to successfully perform a perfusion 3-D cell culture of mesenchymal stem cells (MSCs) for up to 16 days. Moreover, we investigated the effects of various cell culture models on the physiology of MSCs. The physiological nature of MSCs can vary with respect to the cell culture model used. Using the perfusion 3-D cell culture format might affect the proliferation and osteogenic differentiation of MSCs. Overall, we have developed a cell culture system that can achieve multi-well microplate-based perfusion 3-D cell culture in an efficient, cost-effective, and user-friendly manner. These features could facilitate the widespread application of perfusion cell culture models for cell-based assays.

  14. Development of a cloud point extraction and spectrophotometry-based microplate method for the determination of nitrite in human urine and blood.

    Science.gov (United States)

    Zhao, Jiao; Lu, Yunhui; Fan, Chongyang; Wang, Jun; Yang, Yaling

    2015-02-05

    A novel and simple method for the sensitive determination of trace amounts of nitrite in human urine and blood has been developed by combination of cloud point extraction (CPE) and microplate assay. The method is based on the Griess reaction and the reaction product is extracted into nonionic surfactant Triton-X114 using CPE technique. In this study, decolorization treatment of urine and blood was applied to overcome the interference of matrix and enhance the sensitivity of nitrite detection. Multi-sample can be simultaneously detected thanks to a 96-well microplate technique. The effects of different operating parameters such as type of decolorizing agent, concentration of surfactant (Triton X-114), addition of (NH4)2SO4, extraction temperature and time, interfering elements were studied and optimum conditions were obtained. Under the optimum conditions, a linear calibration graph was obtained in the range of 10-400 ng mL(-1) of nitrite with limit of detection (LOD) of 2.5 ng mL(-1). The relative standard deviation (RSD) for determination of 100 ng mL(-1) of nitrite was 2.80%. The proposed method was successfully applied for the determination of nitrite in the urine and blood samples with recoveries of 92.6-101.2%.

  15. Review of palaeozygopleurid gastropods (Palaeozygopleuridae, Gastropoda) from Devonian strata of the Perunica microplate (Bohemia), with a re-evaluation of their stratigraphic distribution, notes on their ontogeny, and descriptions of new taxa.

    Science.gov (United States)

    Frýda, Jiři; Ferrová, Lenka; Frýdová, Barbora

    2013-01-01

    Review of all species of the family Palaeozygopleuridae Horný, 1955 (Gastropoda) known from the Perunica microplate (Bohemia) is presented with a description of three new species, Palaeozygopleura lukesi sp. nov., Cimrmaniela sveraki gen. et sp. nov. and Cimrmaniela smoljaki gen. et sp. nov. The stratigraphic distributions of the most of Bohemian palaeozygopleurid species are either corrected or refined, based on new records or modern stratigraphic studies. A complete list of the geographic occurrences of all known palaeozygopleurid gastropods from the Perunica microplate is also given together with notes on their ontogeny.

  16. Juan Puma, el hijo del oso. Cuento quechua de La Jalca, Chachapoyas

    Directory of Open Access Journals (Sweden)

    1997-01-01

    Full Text Available JUAN PUMA, LE FILS DE L'OURS . CONTE QUECHUA DE LA JALCA, CHACHAPOYAS. Une des fêtes les plus importantes de la communauté de La Jalca, Province de Chachapoyas, Amazonas, est celle de la Saint-Pierre où l’on exécute une danse de l’ours très semblable à celle des ukukus du sud péruvien. Le narrateur du récit que nous publions ici assimile ce rite à l’histoire de Juan Oso (Jean de l’Ours très connue dans le monde andin. L’importance de l’ours dans cette culture des hautes terres amazoniennes est fondamentale. En quechua local, l’ours s’appelle “puma” et partage avec le puma de la forêt les caractéristiques de puissance effrayante et de pouvoirs surnaturels. Le fils de l’ours, Juan, évoque les relations ambiguës liant les “chrétiens” des hautes terres d’Amazonas et les “sauvages” de la forêt. Una de las fiestas más importantes de la comunidad de La Jalca, Provincia de Chachapoyas, Amazonas, es la de San Pedro en la que se representa un baile del oso muy semejante al baile de los ukukus del sur peruano. El narrador del cuento que publicamos aquí asimila este rito a la historia de Juan Oso, muy conocido en el mundo andino. La importancia del oso en esta cultura de la ceja de selva alta es fundamental. En quechua local, el oso se llama “puma” y comparte con el puma de la selva las características de fuerza temible y poderes sobrenaturales. El hijo del oso, Juan, evoca las relaciones ambiguas existentes entre los “cristianos” de la serranía de Amazonas y los “chunchos” de la selva. JUAN PUMA, THE BEAR'S SON. A QUECHUA TALE FROM LA JALCA, CHACHAPOYAS. One of the most important feasts of the community of La Jalca, Province of Amazonas, is that of Saint Peter in which a bear dance similar to the southern Peruvian dance of the ukukus is performed. In the story published here, the narrator assimilates this rite with the tale of Juan Oso, another story which is well known throughout the Andes. The importance of bears

  17. ["Is it an animal inside? "Melanie Klein's unpublished Don Juan Paper (1939)].

    Science.gov (United States)

    Frank, Claudia

    2008-01-01

    Klein had been asked to contribute an article to the birthday number of the International Journal for Jones. The author outlines how she hurriedly wrote a text about Don Juan which, however, was rejected by the editor. Essential parts of it are presented in German translation. The manuscript is discussed in the context of Klein's published work as well as of the relevant contemporary literature. In Klein's view, Don Juan's genitality is determined by oral impulses and fears. By his manic acting out he attempts to ward off a depressive break-down. The paper ends with some reflections about why Klein--ontrary to her intention--failed to revise her manuscript for later publication.

  18. An unpublished Roman inscription from Cortinal de San Juan (Salvatierra de Tormes, Salamanca

    Directory of Open Access Journals (Sweden)

    Verónica PÉREZ DE DIOS

    2015-11-01

    Full Text Available In this paper is presented a slate with a Roman inscription found in archaeological survey work conducted in September 2014 in Cortinal de San Juan (Salvatierra de Tormes. This site has been, since its discovery and cataloging, a place of reference for the late Roman and Visigothic settlement Southeast Salamanca. Currently, this archaeological site is partially flooded by the Santa Teresa swamp. The main feature of this Roman inscription is that the slate support isn’t usual in the chronology proposed for such inscription. Despite the fact that other archaeological materials of Cortinal de San Juan are associated with late Roman or Visigothic chronocultural context, the type of capital letter, the large size of the letter, and the parallels established with other finds of similar chronology suggest this inscription could be part of a Roman anthroponym as the most successful interpretive hypothesis.

  19. Juan Ramón Jiménez y el mercado editorial

    OpenAIRE

    Sánchez García, Raquel

    2003-01-01

    Este trabajo pretende analizar las vinculaciones de Juan Ramón Jiménez con el mundo de la edición. El objetivo es ver el camino que siguió para dar a conocer sus versos: con qué empresas editoriales contrató la publicación de sus libros, cómo logró los medios para llevarlo a cabo y de qué canales se sirvió para distribuirlos. Se trata de analizar un fenómeno muy frecuente entre los autores del primer tercio del siglo XX: la autoedición. También se abordará la participación de Juan Ramón en al...

  20. Traces of the Sacred in the Poetry of Juan Eduardo Cirlot

    OpenAIRE

    2012-01-01

    Juan Eduardo Cirlot (1916-1973), a peculiar and iconoclastic poet, creates with Bronwyn, a collection of poems, one of the most suggestive and attractive imaginary universes in 20th century Spanish literature. Interested in musical serialism, cabbala, simbolism and almost all ancient and modern literary traditions, Cirlot chooses topics from all these sources to combine them with his own spiritual search. Our critical method is based in symbolic analysis the study of the nature and meaning of...

  1. Juan Yacht Design:ITesting LES turbulence models in race boat sails

    OpenAIRE

    Herbert Owen

    2014-01-01

    The objective of this project is to implement LES turbulence models outside the academic world to simulate flow around sailsIto replace RANS models that are the standard in the industry. The implementation and testing in the finite code ALYA is done by the Barcelona Supercomputing Center so that Juan Yatch Design SL (JYD) can appreciate the advantages of using a LES formulation for their problem.The obtained results show that LES can provide significant advantages over RANS simula...

  2. Geologic map of the Cochetopa Park and North Pass Calderas, northeastern San Juan Mountains, Colorado

    Science.gov (United States)

    Lipman, Peter W.

    2012-01-01

    The San Juan Mountains in southwestern Colorado have long been known as a site of exceptionally voluminous mid-Tertiary volcanism, including at least 22 major ignimbrite sheets (each 150-5,000 km3) and associated caldera structures active at 33-23 Ma. Recent volcanologic and petrologic studies in the San Juan region have focused mainly on several ignimbrite-caldera systems: the southeastern area (Platoro complex), western calderas (Uncompahgre-Silverton-Lake City), and the central cluster (La Garita-Creede calderas). Far less studied has been the northeastern San Juan region, which occupies a transition between earlier volcanism in central Colorado and large-volume younger ignimbrite-caldera foci farther south and west. The present map is based on new field coverage of volcanic rocks in seventeen 7.5' quadrangles in northeastern parts of the volcanic field, high-resolution age determinations for 120 new sites, and petrologic studies involving several hundred new chemical analyses. This mapping and the accompanying lab results (1) document volcanic evolution of the previously unrecognized North Pass caldera and the morphologically beautifully preserved but enigmatic Cochetopa basin, including unique features not previously described from ignimbrite calderas elsewhere; (2) provide evidence for a more rapid recurrence of large ignimbrite eruptions than previously known elsewhere; (3) quantify the regional time-space-volume progression from the earlier Sawatch magmatic trend southward into the San Juan region; and (4) permit more rigorous comparison between the broad mid-Tertiary magmatic belt in the western U.S. Cordillera and the type continental-margin arc volcanism in the central Andes.

  3. Juan García de Miranda : pinturas religiosas en conjuntos madrileños (II

    Directory of Open Access Journals (Sweden)

    María Teresa Jiménez Priego

    1995-01-01

    Full Text Available Juan de Miranda, en sus prolongados contactos en Madrid y Alcalá de Henares con la orden franciscana, se familiarizó con los temas favoritos de la Orden y su época: la vida de los santos más insignes (San Diego, San Pascual Bailón.... Fruto de esta influencia parecen ser dos cuadros sobre la vida de San Pascual Bailón que habitualmente se le han atribuido.

  4. The paradigm of paraglacial megafans of the San Juan river basin, Central Andes, Argentina

    Science.gov (United States)

    Suvires, Graciela M.

    2014-11-01

    The spatial distribution and several morphometric characteristics of the Quaternary alluvial fans of the San Juan River, in the province of San Juan, at the Central and Western part of Argentina, have been studied to classify them as paraglacial megafans, as well to ratify its depositional environmental conditions. The high sedimentary load exported by San Juan river from the Central Andes to the foreland depressions is estimated about 3,682,200 hm3. The large alluvial fans of Ullum-Zonda and Tulum valleys were deposited into deep tectonic depressions, during the Upper Pleistocene deglaciation stages. The outcome of collecting remotely sensed data, map and DEM data, geophysical data and much fieldwork gave access to morphometric, morphographic and morphogenetic data of these alluvial fans. The main drainage network was mapped on processed images using QGis (vers.2.0.1). Several fan morphometric parameters were measured, such as the size, the shape, the thickness, the surface areas and the sedimentary volume of exported load. The analyzed fans were accumulated in deep tectonic depressions, where the alluvium fill reaches 700 to 1200 m thick. Such fans do not reach the large size that other world megafans have, and this is due to tectonic obstacles, although the sedimentary fill average volume surpasses 514,000 hm3. The author proposes to consider Ullum-Zonda and Tulum alluvial fans as paraglacial megafans. According to the stratigraphic relationships of the tropical South American Rivers, the author considers that the San Juan paraglacial megafans would have occurred in the period before 24 ka BP , possibly corresponding to Middle Pleniglacial (ca 65-24ka BP). They record colder and more humid conditions compared with the present arid and dry conditions.

  5. El paisaje eólico de la llanura aluvial de San Juan (Llanura Manchega Central)

    OpenAIRE

    Pérez-González, Alfredo; Aleixandre, T.; Pinilla, A.; Gallardo, J.

    1983-01-01

    El campo dunar de la Llanura aluvial de San Juan, esta caracterizado por la presencia de dunas arcillosas, limosas o limo-arcillosas. Morfológicamente, son dunas de tipo parabólico y otras con aspecto "cónico", "transverso" o "longitudinal", construidas por paleovientos efectivos que provenían del W y SI-J.. Finalmente, se discute la edad y origen de estos complejos dunares.

  6. Paleoenvironmental interpretation using fossil record: San Juan Raya Formation, Zapotitlán basin, Puebla, Mexico

    OpenAIRE

    2014-01-01

    The San Juan Raya Formation is world-wide recognized because of the high diversity and abundance of fossils. In this study nine biofacies, three ichnofacies and ten lithofacies were recognized and interpreted on the basis of the influence of several environmental factors such as water depth change, sedimentation rate, water salinity and substrate consistency. Among these factors, salinity variations were apparently crucial for developing and replacement of the different biofacies. Most of bio...

  7. Juan César García: social medicine as project and endeavor.

    Science.gov (United States)

    Nunes, Everardo Duarte

    2015-01-01

    This paper analyses some aspects of the trajectory of the Argentinian physician and sociologist Juan César García (1932-1984) in the field of Latin American Social Medicine. Three dimensions constituting his basic orientations are highlighted: the elaboration of systematic and reflective social thought; a critical attitude in questioning teaching and professional practices; a commitment to the institutionalization and dissemination of health knowledge.

  8. Alluvial fan flooding in the Department of Pocito, Province of San Juan, Argentina

    Institute of Scientific and Technical Information of China (English)

    LauraPatriciaPerucca; JuanParedes

    2004-01-01

    The study consists of the identification of landforms subject to alluvial fan flooding in active sectors of the Zonda range piedmont. In the Department of Pocito, located about 5 km southwest of San Juan City, a series of alluvial fans have been identified. These alluvial fans are located downstream of the natural drainage basins covering an area of approximately 130 km2 towards the eastern slopes of the Zonda range at a median elevation of 2,000 m a.s.l.

  9. Reflections on Don Juan and on the utility of the unhappy love affair.

    Science.gov (United States)

    Bergel, Ernest

    2011-12-01

    Based on an unusual clinical experience of a teenage boy in child psychotherapy, two conclusions are proposed: (1) that the extremely unhappy, early love affairs that occur in most men's lives serve a valuable function in helping them separate from their mothers sufficiently to be able to realistically relate to appropriate marriage partners, and (2) that some Don Juans start new relationships in order to break them off, rather than the reverse.

  10. La fauna simbólica en los cuentos de Juan Rulfo

    OpenAIRE

    Jara Murillo, Carla Victoria

    2015-01-01

    El objetivo principal de este artículo es el análisis de la simbología animal en los cuentos de El llano en llamas, de Juan Rulfo. Se hace un recorrido por los dieciséis relatos que componen el volumen para determinar el funcionamiento de una serie de imágenes, metáforas y símbolos de referentes animales que establecen una isotopía.

  11. Juan Caramuel y la probable arquitectura. Jorge Fernández-Santos

    OpenAIRE

    Gil Saura, Yolanda

    2016-01-01

    Reseña del libro de Jorge Fernández-Santos Ortiz-Iribas, Juan Caramuel y la probable arquitectura. Centro de Estudios Europa Hispánica, Madrid, 2014, 592 págs., 114 ilustraciones en color. Analiza la aportación del libro en relación a la historiografía de la arquitectura barroca española.

  12. Geologic and hydrologic characterization of coalbed-methane reservoirs in the San Juan basin

    Energy Technology Data Exchange (ETDEWEB)

    Kaiser, W.R. (Univ. of Texas, Austin, TX (United States)); Ayers, W.B. Jr.

    1994-09-01

    Fruitland coals are best developed in the north-central part of the San Juan basin. Coal distribution is controlled by shoreline and fluvial depositional settings. Hydraulic gradient, pressure regime, and hydrochemistry reflect regional permeability contrasts. The most productive (>1 MMcf/D) coalbed wells occur along a structural hinge line in association with a regional permeability barrier (no-flow boundary) at the basin center.

  13. Temporal Geochemistry Data from Five Springs in the Cement Creek Watershed, San Juan County, Colorado

    Science.gov (United States)

    Johnson, Raymond H.; Wirt, Laurie; Leib, Kenneth J.

    2008-01-01

    Temporal data from five springs in the Cement Creek watershed, San Juan County, Colorado provide seasonal geochemical data for further research in the formation of ferricretes. In addition, these data can be used to help understand the ground-water flow system. The resulting data demonstrate the difficulty in gathering reliable seasonal data from springs, show the unique geochemistry of each spring due to local geology, and provide seasonal trends in geochemistry for Tiger Iron Spring.

  14. Tradición y modernidad: la Virgen de San Juan de los Lagos

    Directory of Open Access Journals (Sweden)

    Anna M. Fernández Poncela

    2008-11-01

    Full Text Available El texto muestra los aspectos modernos y tradicionales de la Virgen de San Juan de los Lagos, imagen y santuario religioso popular en el estado de Jalisco, México. Es una devoción con origen tradicional que va en aumento, que aglutina la identidad regional, la religión y el comercio, y se expande entre los migrantes a Estados Unidos.

  15. Juan Soreda y las tablas del antiguo retablo de Luzón (Guadalajara

    Directory of Open Access Journals (Sweden)

    Ramos Gómez, F. Javier

    2002-09-01

    Full Text Available En el presente artículo se da noticia del hallazgo en la iglesia de Torremocha del Pinar (Guadalajara, de la obra documentada más antigua de Juan Soreda: el retablo mayor de Luzón. Todo lo referente al modo en que las cinco tablas llegaron desde Luzón hasta la citada localidad de Torremocha del Pinar se tratará más adelante; por el momento he de señalar que tras este descubrimiento es posible construir con mayor solidez la etapa temprana de Juan Soreda. Las cinco escenas que se dan a conocer son inéditas y en ellas se aprecia que Soreda es, ya en la primera década del siglo XVI, un pintor con marcada personalidad que conoce y utiliza el lenguaje que imponían en Castilla Pedro Berruguete, Juan de Borgoña o Santa Cruz.…

  16. Assessing Climate Variability Effects on Dengue Incidence in San Juan, Puerto Rico

    Directory of Open Access Journals (Sweden)

    Pablo Méndez-Lázaro

    2014-09-01

    Full Text Available We test the hypothesis that climate and environmental conditions are becoming favorable for dengue transmission in San Juan, Puerto Rico. Sea Level Pressure (SLP, Mean Sea Level (MSL, Wind, Sea Surface Temperature (SST, Air Surface Temperature (AST, Rainfall, and confirmed dengue cases were analyzed. We evaluated the dengue incidence and environmental data with Principal Component Analysis, Pearson correlation coefficient, Mann-Kendall trend test and logistic regressions. Results indicated that dry days are increasing and wet days are decreasing. MSL is increasing, posing higher risk of dengue as the perimeter of the San Juan Bay estuary expands and shorelines move inland. Warming is evident with both SST and AST. Maximum and minimum air surface temperature extremes have increased. Between 1992 and 2011, dengue transmission increased by a factor of 3.4 (95% CI: 1.9–6.1 for each 1 °C increase in SST. For the period 2007–2011 alone, dengue incidence reached a factor of 5.2 (95% CI: 1.9–13.9 for each 1 °C increase in SST. Teenagers are consistently the age group that suffers the most infections in San Juan. Results help understand possible impacts of different climate change scenarios in planning for social adaptation and public health interventions.

  17. Assessing climate variability effects on dengue incidence in San Juan, Puerto Rico.

    Science.gov (United States)

    Méndez-Lázaro, Pablo; Muller-Karger, Frank E; Otis, Daniel; McCarthy, Matthew J; Peña-Orellana, Marisol

    2014-09-11

    We test the hypothesis that climate and environmental conditions are becoming favorable for dengue transmission in San Juan, Puerto Rico. Sea Level Pressure (SLP), Mean Sea Level (MSL), Wind, Sea Surface Temperature (SST), Air Surface Temperature (AST), Rainfall, and confirmed dengue cases were analyzed. We evaluated the dengue incidence and environmental data with Principal Component Analysis, Pearson correlation coefficient, Mann-Kendall trend test and logistic regressions. Results indicated that dry days are increasing and wet days are decreasing. MSL is increasing, posing higher risk of dengue as the perimeter of the San Juan Bay estuary expands and shorelines move inland. Warming is evident with both SST and AST. Maximum and minimum air surface temperature extremes have increased. Between 1992 and 2011, dengue transmission increased by a factor of 3.4 (95% CI: 1.9-6.1) for each 1 °C increase in SST. For the period 2007-2011 alone, dengue incidence reached a factor of 5.2 (95% CI: 1.9-13.9) for each 1 °C increase in SST. Teenagers are consistently the age group that suffers the most infections in San Juan. Results help understand possible impacts of different climate change scenarios in planning for social adaptation and public health interventions.

  18. Juan de Fuca slab geometry and its relation to Wadati-Benioff zone seismicity

    Science.gov (United States)

    McCrory, Patricia A.; Blair, J. Luke; Waldhause, Felix; Oppenheimer, David H.

    2012-01-01

    A new model of the subducted Juan de Fuca plate beneath western North America allows first-order correlations between the occurrence of Wadati-Benioff zone earthquakes and slab geometry, temperature, and hydration state. The geo-referenced 3D model, constructed from weighted control points, integrates depth information from earthquake locations and regional seismic velocity studies. We use the model to separate earthquakes that occur in the Cascadia forearc from those that occur within the underlying Juan de Fuca plate and thereby reveal previously obscured details regarding the spatial distribution of earthquakes. Seismicity within the slab is most prevalent where the slab is warped beneath northwestern California and western Washington suggesting that slab flexure, in addition to expected metamorphic dehydration processes, promotes earthquake occurrence within the subducted oceanic plate. Earthquake patterns beneath western Vancouver Island are consistent with slab dehydration processes. Conversely, the lack of slab earthquakes beneath western Oregon is consistent with an anhydrous slab. Double-differenced relocated seismicity resolves a double seismic zone within the slab beneath northwestern California that strongly constrains the location of the plate interface and delineates a cluster of seismicity 10 km above the surface that includes the 1992 M7.1 Mendocino earthquake. We infer that this earthquake ruptured a surface within the Cascadia accretionary margin above the Juan de Fuca plate. We further speculate that this earthquake is associated with a detached fragment of former Farallon plate. Other subsurface tectonic elements within the forearc may have the potential to generate similar damaging earthquakes.

  19. Geologic Map of the Central San Juan Caldera Cluster, Southwestern Colorado

    Science.gov (United States)

    Lipman, Peter W.

    2006-01-01

    The San Juan Mountains are the largest erosional remnant of a composite volcanic field that covered much of the southern Rocky Mountains in middle Tertiary time. The San Juan field consists mainly of intermediate-composition lavas and breccias, erupted about 35-30 Ma from scattered central volcanoes (Conejos Formation) and overlain by voluminous ash-flow sheets erupted from caldera sources. In the central San Juan Mountains, eruption of at least 8,800 km3 of dacitic-rhyolitic magma as nine major ash flow sheets (individually 150-5,000 km3) was accompanied by recurrent caldera subsidence between 28.3 Ma and about 26.5 Ma. Voluminous andesitic-dacitic lavas and breccias erupted from central volcanoes prior to the ash-flow eruptions, and similar lava eruptions continued within and adjacent to the calderas during the period of more silicic explosive volcanism. Exposed calderas vary in size from 10 to 75 km in maximum dimension; the largest calderas are associated with the most voluminous eruptions.

  20. Sediment-bound trace metals in Golfe-Juan Bay, northwestern Mediterranean: Distribution, availability and toxicity.

    Science.gov (United States)

    Tiquio, Ma Gregoria Joanne; Hurel, Charlotte; Marmier, Nicolas; Taneez, Mehwish; Andral, Bruno; Jordan, Norbert; Francour, Patrice

    2017-05-15

    The concentration, potential mobility, cation exchange capacity and toxicity of eight sediment-bound metals in Golfe-Juan Bay, France were examined. Results revealed significant spatial gradient of metal contamination along Golfe-Juan coast. The distribution and concentration of the metals appear to be influenced by the geochemical properties of the sediment, proximity to anthropogenic sources and general water circulation in the bay. The portion of trace metals found in the exchangeable, carbonate, oxidizable and reducible fractions of the sediment constitute 31%-58% of the total sediment-bound trace metal content, suggesting significant potential for remobilization of metals into the water column. Pb and Ni content of the sediment exceed the limits of the French marine sediment quality. Whole sediment extracts showed acute toxicity to marine rotifers. This study concludes that monitoring and management of sediment-bound trace metals in Golfe-Juan Bay are important so as not to underestimate their availability and risk to the marine ecosystems. Copyright © 2017 Elsevier Ltd. All rights reserved.

  1. Geologic map of the central San Juan caldera cluster, southwestern Colorado

    Science.gov (United States)

    Lipman, Peter W.

    2006-01-01

    The San Juan Mountains are the largest erosional remnant of a composite volcanic field that covered much of the southern Rocky Mountains in middle Tertiary time. The San Juan field consists mainly of intermediate-composition lavas and breccias, erupted about 35-30 Ma from scattered central volcanoes (Conejos Formation) and overlain by voluminous ash-flow sheets erupted from caldera sources. In the central San Juan Mountains, eruption of at least 8,800 km3 of dacitic-rhyolitic magma as nine major ash flow sheets (individually 150-5,000 km3) was accompanied by recurrent caldera subsidence between 28.3 Ma and about 26.5 Ma. Voluminous andesitic-dacitic lavas and breccias erupted from central volcanoes prior to the ash-flow eruptions, and similar lava eruptions continued within and adjacent to the calderas during the period of more silicic explosive volcanism. Exposed calderas vary in size from 10 to 75 km in maximum dimension; the largest calderas are associated with the most voluminous eruptions.

  2. Mantle flow geometry from ridge to trench beneath the Gorda-Juan de Fuca plate system

    Science.gov (United States)

    Martin-Short, Robert; Allen, Richard M.; Bastow, Ian D.; Totten, Eoghan; Richards, Mark A.

    2015-12-01

    Tectonic plates are underlain by a low-viscosity mantle layer, the asthenosphere. Asthenospheric flow may be induced by the overriding plate or by deeper mantle convection. Shear strain due to this flow can be inferred using the directional dependence of seismic wave speeds--seismic anisotropy. However, isolation of asthenospheric signals is challenging; most seismometers are located on continents, whose complex structure influences the seismic waves en route to the surface. The Cascadia Initiative, an offshore seismometer deployment in the US Pacific Northwest, offers the opportunity to analyse seismic data recorded on simpler oceanic lithosphere. Here we use measurements of seismic anisotropy across the Juan de Fuca and Gorda plates to reconstruct patterns of asthenospheric mantle shear flow from the Juan de Fuca mid-ocean ridge to the Cascadia subduction zone trench. We find that the direction of fastest seismic wave motion rotates with increasing distance from the mid-ocean ridge to become aligned with the direction of motion of the Juan de Fuca Plate, implying that this plate influences mantle flow. In contrast, asthenospheric mantle flow beneath the Gorda Plate does not align with Gorda Plate motion and instead aligns with the neighbouring Pacific Plate motion. These results show that asthenospheric flow beneath the small, slow-moving Gorda Plate is controlled largely by advection due to the much larger, faster-moving Pacific Plate.

  3. Navegación costarricense por el río San Juan

    Directory of Open Access Journals (Sweden)

    Ricardo Wheelock Román

    2002-08-01

    Full Text Available El Estado de Nicaragua afirma que el Laudo Cleveland zanjó todas las cuestiones relativas a los límites fronterizos terrestres con Costa Rica, a la vez que no se reconoce la sentencia de la CJC de 1916 ya que se extralimitó en sus funciones. Por el Tratado Jerez-Cañas, Costa Rica sólo puede servirse del Río San Juan para "objetos de comercio" y entrando por San Juan del Norte, para que lleguen al interior de Costa Rica, el Tratado no especifica la viceversa, por lo que no puede ser usado para fines turísticos, vía de navegación o de abastecimiento 0 que el derecho de navegar "con objetos de comercio" se pueda , como extensión de soberanía y jurisdicción costarricense a los buques y personas de ese país que navegan por el Río San Juan, 10 que resultaría en negación de la soberanía de Nicaragua

  4. Fray Francisco de San Juan, a spanish missionary in Bahia in 1624

    Directory of Open Access Journals (Sweden)

    Iglesias Magalhães, Pablo Antonio

    2011-06-01

    Full Text Available The present article discloses the existence of an unpublished manuscript on the netherlandish invasion the Bahia in 1624. The author of the manuscript is a Spanish missionary called Fray Francisco de San Juan, who presents the best relation of the first months of the war, disclosing unknown details of the resistance against the invaders until the death of the Bishop D. Marcos Teixeira.

    El presente artículo divulga la existencia de un manuscrito inédito sobre la invasión neerlandesa en la Bahia en 1624. Su autor es el misionario español Fray Francisco de San Juan, que presenta la mejor relación de los primeros meses de la guerra, divulgando los detalles desconocidos de la resistencia contra los invasores hasta la muerte del Obispo D. Marcos Teixeira. [pt] O presente artigo divulga a existência de um manuscrito inédito sobre a invasão neerlandesa na Bahia em 1624. Seu autor é o missionário espanhol Frei Francisco de San Juan, que apresenta a melhor relação dos primeros meses da guerra, divulgando os detalhes desconhecidos da resistência contra os invasores até a morte do bispo D. Marcos Teixeira.

  5. Oil-bearing sediments beneath San Juan volcanics - Colorado's newest frontier

    Energy Technology Data Exchange (ETDEWEB)

    Gries, R.R.

    1985-05-01

    During the Tertiary, the western part of the northern Sange de Cristo Range dropped 16,000 ft (4877 m) to become what is now known as the San Luis basin. The foreland basin formerly adjacent to and west of the range remained intact but was subsequently concealed by 10,000 ft (3048 m) of volcanic deposits. The existence of this concealed basin, a northeastern arm of the San Juan basin, was first suggested by Vincent Kelly who named it the San Juan sag. Oil, which was generated in the underlying Mancos Shale, migrated upward into vesicles and fractures in volcanic rocks. In at least two places, oil is currently seeping onto the volcanic surface or into overlying soil. These oil occurrences encouraged geologic and geophysical exploration and have led to confirmation by drilling that the basin exists. Porous reservoirs in both tertiary sedimentary rocks and volcanic rocks overlie a 2000 ft (610 m) Cretaceous Mancos Shale source rock. Within the Mancos Shale are fractured reservoirs, volcanic sills that have reservoir potential where fractured or porous, and stray sandstones. The Dakota Formation underlies the Mancos Shale and is about 200 ft (61 m) thick in this area. In addition, the Jurassic section has potential for source rocks in the Todilto Formation and reservoir rocks in the Entrada and Junction Creek Sandstones. The San Juan sag, a newly discovered basin of 2600 miS (6734 kmS) is a frontier for Colorado oil and gas exploration.

  6. Juan Gonzalo Restrepo Londoño. Semblanzas de un empresario Juan Gonzalo Restrepo Londoño. Semblanzas de un empresario

    Directory of Open Access Journals (Sweden)

    Jairo Andrés Campuzano Hoyos

    2008-06-01

    Full Text Available Es este un breve recorrido por la vida de Juan Gonzalo Restrepo Londoño,quien además de tener la fortuna de crecer en el seno de una familia tradicionalantioqueña, fue haciéndose portador de invaluables conocimientos que lepermitirían engranarse exitosamente en la maquinaria empresarial colombianadurante la segunda mitad del siglo xx. La institución de la familia, el conocimientodel entorno natural y social, y una marcada propensión al servicio de los interesesregionales, serán, entre otros, los elementos que caractericen y hagan comprensiblelas ejecutorias de este empresario multifacético e innovador.This is a brief journey through the life of Juan Gonzalo Restrepo Londoño. Besides having the fortune of growing up within the heart of a traditional family of Antioquia, he became bearer of invaluable knowledge since his youth, which allowed him to engage successfully in the entrepreneurial machinery of the region during the second half of the XXth century.The institution of the family, the knowledge of the natural and social context and a marked tendency to serve the interests of a region would be, among others, the elements to characterize and make intelligible the actions of this versatile and innovative businessman.

  7. La Librería de Juan Polo en Murcia a mediados del siglo XVIII Juan Polo's library in Murcia in the middle of the XVIII Century

    Directory of Open Access Journals (Sweden)

    Amparo García Cuadrado

    2009-12-01

    Full Text Available A través del análisis del inventario de los bienes dejados a su muerte por el librero Juan Polo, junto a la utilización de otras fuentes archivísticas, se retrata el perfil vital y las actividades desarrolladas por este profesional del libro en la Murcia de mediados del siglo XVIII. La trascripción e identificación de los libros y otros impresos menores reseñados en el inventario y, sobre todo, la cuantía de ejemplares por título ha permitido abordar el tipo de clientela de la librería y la aceptación o el éxito de determinados productos editoriales, siempre en consonancia con unas necesidades prácticas, formativas o recreativas y con unos hábitos lectores fácilmente reconocibles.The profile and activities carried out by the bookseller Juan Polo during the XVIII century are analyzed through the inventory of assets and other archival sources which remained after his death. The identification and transcription of books and other minor works recorded in the inventory, and above all the quantity of existing copies of each title, allow us to also depict the profile of regular clients, and to assess the degree of success attained by certain publishing products. Practical, recreational or educational needs were also kept in mind in order to acknowledge easily recognizable reader's habits.

  8. Parásitos en perros de San Juan Bautista, Isla Robinson Crusoe, Chile Parasites in dogs from San Juan Bautista, Robinson Crusoe Island, Chile

    Directory of Open Access Journals (Sweden)

    D González-Acuña

    2008-01-01

    Full Text Available Se estudió la fauna parasitaria de 40 perros en el poblado de San Juan Bautista, Isla Robinson Crusoe, Chile. El 50% (n = 20 de los perros presentó huevos de tipo Ancylostomideos, Strongyloideos y/o de especies Isospora sp. y Cystoisospora canis. No se encontraron muestras positivas a cestodos. El 100% de los perros presentó alguno de los siphonapteros Ctenocephalides canis, C. felis y/o Pulex irritans. En un perro se aisló un ejemplar de la garrapata café del perro Rhipicephalus sanguineus (2,5%. Se discute la importancia de los presentes resultados.The parasitological fauna of 40 dogs was studied in San Juan Bautista, Robinson Crusoe Island, Chile. 50% (n = 20 of the dogs had eggs of the Ancylostomid, Strongylid type and/or the oocysts of Isospora sp. and Cystoisospora canis. No positive samples of cestodes were found. 100% of the dogs were parasited by the fleas Ctenocephalides canis, C. felis and/or Pulex irritans. One brown dog tick, Rhipicephalus sanguineus, was found in one dog (2.5%. The importance of these findings is discussed.

  9. The Literary Criticism and Memoirs of Juan Ramón Jiménez

    Directory of Open Access Journals (Sweden)

    Allen W. Phillips

    1983-01-01

    Full Text Available Equally as demanding of others as he was of himself, Juan Ramón Jiménez conceived of literary criticism as a serious and exacting task. The critic and the poet, standing side by side, are devoted to complementary activities of mutual enrichment. However fragmentary and partial the critical opinions of Juan Ramón may be (also outspoken and polemical in nature, they are invaluable as a personal historical and aesthetic guide to about fifty or sixty years of Hispanic literary development (1900-1960. Not to take them into account is to fail to recognize a highly important aspect of his total artistic personality. These varied critical texts are a product of a first rate intelligence and the sensibility of a writer of consummate discrimination who was endowed not only with an excellent memory but also a very special talent for appreciating the authentic. Juan Ramón as a critic is quick to praise (San Juan, Bécquer, Dario, Unamuno and Machado and at the same time strong in his censure of certain contemporaries. Several recently collected volumes of miscellaneous critical materials have resolved the bibliographical muddle for the initial study of this fundamental aspect of the poet, but still particularly important are the expressionistic portraits of Españoles de tres mundos , longer tributes to Valle, Ortega and Villaespesa as well as the extensive lectures delivered in his latter years. Examination of these pages gives us an historical and creative overview of the period in which he lived and worked in addition to original considerations about the evolution of Hispanic poetry. Of course, one of the constant focal points of his literary criticism was the modernist epoch of his early days, a movement or attitude which he considered to be a modern twentieth century renaissance. Together with detailed study of these two areas of historical and aesthetic nature, in the ensuing pages some conclusions are pointed out as to the essence of poetry

  10. Divergent Ridge Features on the Juan de Fuca and Gorda Ridges

    Science.gov (United States)

    Eaton, M. E.; Sautter, L.; Steele, M.

    2014-12-01

    Multibeam data collected using a Kongsberg EM122 sonar system on the NOAA ship R/V Marcus G. Langseth led by chief scientist Douglas Toomey (University of Oregon) in 2009 and with a Simrad EM302 sonar system on two NOAA ship Okeanos Explorer cruises led by chief scientists James Gardner (University of New Hampshire) and Catalina Martinez (University of Rhode Island) in 2009 show the morphology of the Juan de Fuca and Gorda Ridges, as well as the Blanco and Mendocino Fracture Zones. These ridges and fracture zones comprise the divergent plate boundary of the eastern edge of the Pacific Plate and the western edges of the Juan de Fuca and Gorda Plates. Both plates are being subducted beneath the western edge of the North American Plate. CARIS HIPS 8.1 software was used to process the multibeam data and create bathymetric images. The ridge axes, located off the coast of Washington and Oregon (USA) adjacent to the Cascadia Basin, indicate obvious signs of spreading, due to the series of faults and rocky ridges aligned parallel to the plate boundaries. Fault and ridge orientations are used to compare the direction of seafloor spreading, and indicate that both the Juan de Fuca Plate and Gorda Plate are spreading in a southeastern direction. Younger ridges from the Gorda Ridge system mapped in the study run parallel to the boundary, however older ridges do not show the same orientation, indicating a change in spreading direction. The presence of hydrothermal vents along the Juan de Fuca Ridge is also evidence of the active boundary, as the vent chimneys are composed of minerals and metals precipitated from the hot water heated by magma from beneath the spreading seafloor. In this study, the data are used to compare and contrast earthquake seismicity and ridge morphologies at a depth range of approximately 762 to 2134 meters. The diverging Pacific, Juan de Fuca, and Gorda Plates along with the San Andreas Fault have potential to increase seismic and volcanic activity around

  11. 侧沟茧蜂触角感觉器的扫描电镜观察%SCANNING ELECTRON MICROSCOPY OF ANTENNAL SENSILLA OF MICROPLITIS SP.

    Institute of Scientific and Technical Information of China (English)

    陈新芳; 高燕; 章潜才

    2004-01-01

    扫描电镜观察表明:侧沟茧蜂Microplitis sp.的触角上存在5种感觉器,分别为毛状感觉器A型、B型、C型,槽状感觉器,锥形乳头状感觉器,刺形感觉器和钟形感觉器.对触角感觉器的形态和分布进行了描述,并对两性间的差别进行了讨论,为研究该蜂寻找生境和寄生行为提供依据.

  12. Toxical effect of Peganum harmala L. leaves on the cotton leaf worm, Spodoptera littoralis Boisd and its parasitoids Microplitis rufiventris Kok.

    Science.gov (United States)

    Shonouda, Mourad; Osman, Salah; Salama, Osama; Ayoub, Amal

    2008-02-15

    The leaf extract and its fractions of Peganum harmala L. have shown pronounced mortal effect, decreased percent pupation and adult emergence of the cotton leaf worm, Spodoptera littoralis Boisd. The third instar larvae fed for two days on treated leaves were more susceptible to plant extract and its ethyl acetate and chloroform fractions. The active lowest concentration (5%) of the leaf fractions of P. harmala showed significant effect on the percentage of emerged adult parasitoids, Microplitis rufiventris Kok. GC/MS analysis showed the major constituent in ethyl acetate fraction was (23S) ethylcholest-5-en-3 beta-ol (28.04%) while those of chloroform fraction were hydroxyfuranocoumarin (Bergaptol) (15.68%), piperidinone (12.08%), thymol (11.82%), phosphoric acid, tributyl ester (9.80%) and trimethyl-nonenol (9.66%). The medicinal plant P. harmala could be carefully applied in integrated pest management due to its strong effect on cotton leaf worm pest.

  13. Contaminants of antropic origin in groundwater San Juan (Argentina). Vulnerability and hydro chemical indicators; Contaminantes de origen antropico en aguas subterraneas San Juan (Argentina)

    Energy Technology Data Exchange (ETDEWEB)

    Fiore, J. M.; Castro, A.; Medici, M. E.; Gimenez, M.; Suero, E.; Turcuman, M. H.

    2002-07-01

    The Tulum Valley aquifer vulnerability and its correlation with the behavior of hydro chemical parameters related to new urban neighborhoods and uncontrolled waste disposal are discussed. A high vulnerability to contamination by substances solved in the recharge water at the San Juan river shore is estimated. A fast transport rate due to the high permeability is foreseen, as well as the existence of contamination sources related to waste disposals and populations without a sewer system. The results show that the mentioned populations and waste disposal sites are located at vulnerable zones of the area. A higher concentration of nitrates and nitrites is observed at the Southeast, due to the short permanency period of the water in the system, and to the reconcentration in zones with less permeable levels. (Author) 19 refs.

  14. Trihalometanes in San Juan city (Argentina) drinking water; Trihalometanos en agua para consumo humano de la ciudad de San Juan (Argentina)

    Energy Technology Data Exchange (ETDEWEB)

    Baudina, O.; Suero, E.; Augusto, M.; Gimenez, M.; Flores, N.

    2001-07-01

    Twenty-four sampling points in the drinking water network of the City of San Juan, Argentina, were chosen in order to investigate the presence of four of the most representative trihalomethanes (THM). The collected data were statistically correlated for evaluating the seasonal variations of these substances, as well as the variations derived from their locations and distance from the drinking water treatment plant. Not any significative difference was observed Between each od the four seasons, neither between the various sampling points located at different distances from the treatment plant. The low concentrations of TOC dissolved in raw water limited the concentrations of THM whose means value did not go beyond the maximum values recommended by WHO. (Author) 22 refs.

  15. Biochemical characterization and immobilization of Erwinia carotovoral-asparaginase in a microplate for high-throughput biosensing of l-asparagine.

    Science.gov (United States)

    Labrou, Nikolaos E; Muharram, Magdy Mohamed

    2016-10-01

    l-Asparaginases (l-ASNase, E.C. 3.5.1.1) catalyze the conversion of l-asparagine to l-aspartic acid and ammonia. In the present work, a new form of l-ASNase from a strain of Erwinia carotovora (EcaL-ASNase) was cloned, expressed in Escherichia coli as a soluble protein and characterized. The enzyme was purified to homogeneity by a single-step procedure comprising ion-exchange chromatography. The properties of the recombinant enzyme were investigated employing kinetic analysis and molecular modelling and the kinetic parameters (Km, kcat) were determined for a number of substrates. The enzyme was used to assemble a microplate-based biosensor that was used for the development of a simple assay for the determination of l-asparagine in biological samples. In this sensor, the enzyme was immobilized by crosslinking with glutaraldehyde and deposited into the well of a microplate in 96-well format. The sensing scheme was based on the colorimetric measurement of ammonia formation using the Nessler's reagent. This format is ideal for micro-volume applications and allows the use of the proposed biosensor in high-throughput applications for monitoring l-asparagine levels in serum and foods samples. Calibration curve was obtained for l-asparagine, with useful concentration range 10-200μΜ. The biosensor had a detection limit of 10μM for l-asparagine. The method's reproducibility was in the order of ±3-6% and l-asparagine mean recoveries were 101.5%.

  16. Ästhetisches Bild und Christliche Mystik im Cántico espiritual des San Juans de la Cruz

    DEFF Research Database (Denmark)

    Kluge, Sofie

    2003-01-01

      In diesem Zusammenhang werde ich, von dem Cántico espiritual des San Juans de la Cruz (1577) ausgehend und zentrale rezeptionsgeschichtliche Probleme einbeziehend, den mystischen Bildbegriff untersuchen. Auf der einen Seite bildet San Juans selbstständige Interpretation des biblischen Hoheliede...

  17. La Obra de Juan Ramón Jiménez como elaboración vivida del concepto del absoluto

    DEFF Research Database (Denmark)

    Jensen, Hans Julio Casado

    2002-01-01

    Juan Ramón Jiménez, spansk litteratur, 20. århundredes litteratur, forhold litteratur og filosofi, forfatterbiografi.......Juan Ramón Jiménez, spansk litteratur, 20. århundredes litteratur, forhold litteratur og filosofi, forfatterbiografi....

  18. 78 FR 37788 - In the Matter of: Juan Ricardo Puente-Paez, Inmate Number #05086-379, FCI McDowell, Federal...

    Science.gov (United States)

    2013-06-24

    ... Bureau of Industry and Security In the Matter of: Juan Ricardo Puente-Paez, Inmate Number 05086-379, FCI..., Juan Ricardo Puente-Paez (``Puente-Paez'') was convicted of violating Section 38 of the Arms Export Control Act (22 U.S.C. 2778 (2006 & Supp. IV 2010)) (``AECA''). Specifically, Puente-Paez was convicted...

  19. Angustia y fe teologal en Kierkegaard y san Juan de la Cruz Anguish and Theological faith in Kierkegaard and San Juan de la Cruz

    Directory of Open Access Journals (Sweden)

    Lucero González Suárez

    2013-03-01

    Full Text Available Se parte del planteamiento general de la perspectiva moderna que aún impera en la interpretación regular de la fe, para señalar sus insuficiencias. Posteriormente, se aborda la oposición entre universalidad ética y particularidad teológica, con base en las observaciones hechas por Kant y Kierkegaard, respectivamente, a fin de determinar el ámbito de la fe y su independencia respecto de la moral. Hecho el deslinde entre el Dios de la fe y el Dios de las filósofos, se describen los rasgos fundamentales de la fe teologal, con base en el testimonio neotestamentario. Finalmente, se expone la concepción místico-religiosa de San Juan de la Cruz acerca de la fe como conocimiento oscuro amoroso, para luego aclarar en qué sentido la fe está íntimamente relacionada con el amor-ágape y la esperanza. Unidad que constituye la esencia de la actitud teologal.Be part of the overall approach of the modern perspective that still prevails in the interpretation regular of faith, to point out its shortcomings. Subsequently, deals with the opposition between ethical universality and particularity theological, based on observations made by Kant and Kierkegaard, respectively, to determine the field of faith and independence from morality. Made the boundary between the God of faith and the God of the philosophers, describes the basic features of theological faith, based on the New Testament witness. Finally, we illustrate the mystical-religious conception of San Juan de la Cruz about faith as loving dark knowledge, then clarify in what sense faith is intimately linked to the love-agape and hope. Unit which is the essence of the theological attitude.

  20. Las Cortes durante la minoría de Juan II de Castilla = The Cortes during the Minority of Juan II of Castile

    Directory of Open Access Journals (Sweden)

    Santiago González Sánchez

    2017-05-01

    Full Text Available El presente artículo estudia la evolución de las Cortes castellanas durante la minoría de Juan II. Nos fijamos en la situación por la que atravesaban esas asambleas en los demás reinos cristianos peninsulares, así como en los problemas que plantea su estudio en Castilla, como el número de reuniones o la diferente denominación que reciben. Nos centramos en las fases, funcionamiento y lugares de reunión. Ampliamos los motivos por los que se convocan y estudiamos los distintos aspectos de los que se ocuparon, destacando las reivindicaciones ciudadanas, entre otras las peticiones de carácter particular y las relaciones de las Cortes con otras instituciones de gobierno de la monarquía. El último apartado se dedica a estudiar la importancia que las Cortes tuvieron para la monarquía y para los estamentos en ellas representados.The present study analyses the evolution of the Castilian Cortes during the minority of Juan II. We will examine the situation of parliamentary assemblies in the other Iberian Christian kingdoms, as well as the problems involved with its study in Castile, such as the number of meetings held or the different names given to each of them. We will focus on the phases, its operation and the places of assembly. We will explore the reasons why the Cortes were convened and study the various issues dealt in them, particularly the demands of the citizens, such as specific requests and the relations between the parliament and other government institutions of the Crown. The last section is dedicated to studying the importance the Cortes had for the monarchy and the estates represented in them.

  1. [The examination of men's wits by Juan Huarte de San Juan, and the dawn of the neurobiology of intelligence in the Spanish renaissance].

    Science.gov (United States)

    Martín-Araguz, A; Bustamante-Martínez, C

    The Spanish renaissance doctor Juan Huarte de San Juan (1529-1588) was the author of a unique and immortal work, The Examination of Men's Wits, the edition princeps of which was printed in Baeza in 1575. Since then it has been reprinted at least 80 times and translated into seven languages, which makes it the most influential Spanish contribution to medicine ever published. In this paper we review the unjustly little-known figure of Huarte as the founder of Neuropsychology, and we also analyse his works from a historical and neuroscientific point of view. Huarte's writings deal with the problem of the organic relations between the brain and understanding, and accept the possible influences exerted by temper on the will within the field of the Neurobiology of Intelligence. Thus, over four centuries ago Huarte became the founder of Differential or Physiological Psychology, Neuropsychology, Eugenics and Career Guidance. Huarte's work not only played a fundamental role in the history and development of the body of neuroscientific knowledge, but has also been a clear (although not always cited) influence on scientists, philosophers and men of letters such as Alarcón, Bacon, Cabanis, Cervantes (whose Don Quixote was inspired by him), Charron, Chomsky, Gall, Goethe, Hume, Kant, Kretchmer, Lessing, Lope de Vega, Montaigne, Montesquieu, Nietzsche, Quevedo, Rousseau, Schopenhauer, Thomasius, Tirso de Molina and Ziegler. In the middle of the 16th century, Huarte and his Examination of Men's Wits, together with the works of the naturalist philosophers Gómez Pereira (Antoniana Margarita, 1554) and Miguel Sabuco (New Philosophy, 1587), constituted the prestigious triumvirate of Spanish Renaissance scholars who, for the first time in history, contemplated the workings of the brain from a point of view that had more to do with science than the supernatural.

  2. Microplacas de titânio em fraturas de tibiotarso em pombos domésticos Titanium microplates for treatment of tibiotarsus fractures in pigeons

    Directory of Open Access Journals (Sweden)

    Aline Silva Gouvêa

    2011-03-01

    grupo 3, houve maior precocidade no apoio do membro e menor grau de envergamento em relação aos outros grupos. Histologicamente, foi verificada a formação de calo ósseo em todos os animais e presença de tecido ósseo ao redor da microplaca e dos microparafusos. Apesar das complicações observadas, as microplacas de titânio são uma opção para osteossíntese de tibiotarso em aves de médio porte.Among the surgical problems in birds, the fractures are the most prevalent. Due to variations in size, weight, peculiar bone anatomy, high incidence of complicated fractures and functional demands of different species and individuals, it is still not possible to establish an ideal method for osteosynthesis of long bones in birds. Titanium microplates commonly used in human maxillofacial surgery have been recently employed in osteosynthesis of birds, but with few results available. The aim of this study was to evaluate the use of titanium microplates in the treatment of tibiotarsus fractures in 30 pigeons (Columba livia , adult males and females, with 7 months old, weighing from 400 to 500. The animals were divided into 3 groups with ten animals each. In group 1, a titanium microplate with 6 holes and a central spacer was used; in group 2, a titanium microplate with 8 holes without a spacer in the center was used; in group 3, a titanium microplate with 8 holes with central spacer was used. After clinical evaluation, the birds were submitted to mid-diaphyseal osteotomy in the right bone for placement of different configurations of the titanium plate, and fixed with titanium microscrews 7mm long. The animals were evaluated clinically and radiographically until 90 days postoperatively. After this period, euthanasia was performed in 2 animals from each group. In all animals was observed bone healing and in group 1 the mean and standard deviation were 32.9±9.9 days, in group 2, 30.8±6.7 days in group 3, 26.6±6.4 days. There was no statistical difference in healing time

  3. El uso de productos del reino mineral en la terapéutica del siglo XVI. El libro de los "Medicamentos simples" de Juan Fragoso (1581) y el "Antidotario" de Juan Calvo (1580)

    OpenAIRE

    Fresquet Febrer, José Luis

    1999-01-01

    This work aims to approach us the use of “mineral products” in medicine during 16th and 17th centuries. To have an idea about the use of minerals in daily practice, we have studied Juan Fragoso’s “Libro de los medicamentos simples” (1581), and Juan Calvo’s “Antidotario” (1580). We have also checked the presence of these substances in Dioscorides “Materia medica”, version of Andrés Laguna, and the “Diccionario de Autoridades”. In addition, but not exhaustively, we have followed the use of thes...

  4. Foundations study using seismic refraction techniques to the Punta Negra Dike, San Juan Province, Argentina; Estudio de fundaciones aplicando el metodo sismico de refraccion en el Dique Punta Negra, Provincia de San Juan, Argentina

    Energy Technology Data Exchange (ETDEWEB)

    Cuneo, Ricardo Horacio; Gonzalez, Juan Miguel; Ponti, Norberto A. [Instituto Sismologico Fernando Volponi (Argentina)

    1995-12-31

    The aim of this work is to determine the thickness and morphology of the deposit that lies over the Graywackes Complex and that will serve as foundations of the prospect Punta Negra Dam, to be built at Punta Negra site, on the right band of the San Juan river, in San Juan, Argentina. Besides, this paper will serve as a complement to studies of geoelectric prospection of the area and will confirm the presence of a paleo river-bed. The depths of the foundation rock and its physical properties were calculated through the refraction seismic method and the stacking method. (author). 5 refs., 4 figs., 1 tab

  5. El uso de productos del reino mineral en la terapéutica del siglo XVI. El libro de los Medicamentos simples de Juan Fragoso (1581) y el Antidotario de Juan Calvo (1580)

    OpenAIRE

    Fresquet Febrer, José Luis

    1999-01-01

    This work aims to approach us the use of "mineral products" in medicine during 16th and 17th centuries. To have an idea about the use of minerals in daily practice, we have studied Juan Fragoso's Libro de los medicamentos simples (1581), and Juan Calvo's Antidotario (1580). We have also checked the presence of these substances in Dioscorides Materia medica, version of Andres Laguna, and the ...

  6. Dengue Fever Trends and Climate Change in San Juan, Puerto Rico

    Science.gov (United States)

    Muller-Karger, F. E.; Mendez-Lazaro, P.; Otis, D. B.; McCarthy, M.; Pena-Orellana, M.

    2014-12-01

    Climate change has important implications for public health. We developed and tested the hypothesis that conditions for dengue fever transmission in San Juan (Puerto Rico, USA) are becoming favorable as a result of meteorological drivers being modified with climate change. Sea level pressure, mean sea level (MSL), wind, sea surface temperature (SST), air surface temperature (AST), rainfall, and confirmed dengue cases were variables examined over the past 30 years, or longer for some variables. Statistical tools used included Principal Component Analysis, Pearson correlation coefficient, Mann-Kendall trend tests, and logistic regressions. Results show that dry days are increasing and that wet days are decreasing. MSL is steadily increasing, which increases the risk of dengue cases along the coast, as the perimeter of the San Juan Bay estuary expands and the shoreline moves inland. Warming is evident in both SST and AST. Maximum and minimum air surface temperature extremes have also increased. Incidence of dengue is accelerating along with environmental change. For example, between 2000-2011, dengue transmission increased by a factor of 3.4 (95% CI: 1.9-6.1) for each 1ºC increase in SST. Between 2007 and 2011, this risk factor increased to 5.2 (95% CI: 1.9-13.9) for every 1ºC increase in SST. An important but difficult to examine problem is how social and economic factors affect such dengue fever transmission rates in light of environmental change. A concern is that the patterns observed in San Juan are representative of potential incidence of dengue virus in other parts of the island of Puerto Rico and in other Caribbean nations. These results help understand patterns of disease spreading, and allow public health officials to evaluate scenarios and interventions intended to mitigate the impacts of climate change.

  7. A heat vulnerability index to improve urban public health management in San Juan, Puerto Rico

    Science.gov (United States)

    Méndez-Lázaro, Pablo; Muller-Karger, Frank E.; Otis, Daniel; McCarthy, Matthew J.; Rodríguez, Ernesto

    2017-02-01

    Increased frequency and length of high heat episodes are leading to more cardiovascular issues and asthmatic responses among the population of San Juan, the capital of the island of Puerto Rico, USA. An urban heat island effect, which leads to foci of higher temperatures in some urban areas, can raise heat-related mortality. The objective of this research is to map the risk of high temperature in particular locations by creating heat maps of the city of San Juan. The heat vulnerability index (HVI) maps were developed using images collected by satellite-based remote sensing combined with census data. Land surface temperature was assessed using images from the Thermal Infrared Sensor flown on Landsat 8. Social determinants (e.g., age, unemployment, education and social isolation, and health insurance coverage) were analyzed by census tract. The data were examined in the context of land cover maps generated using products from the Puerto Rico Terrestrial Gap Analysis Project (USDA Forest Service). All variables were set in order to transform the indicators expressed in different units into indices between 0 and 1, and the HVI was calculated as sum of score. The tract with highest index was considered to be the most vulnerable and the lowest to be the least vulnerable. Five vulnerability classes were mapped (very high, high, moderate, low, and very low). The hottest and the most vulnerable tracts corresponded to highly built areas, including the Luis Munoz International Airport, seaports, parking lots, and high-density residential areas. Several variables contributed to increased vulnerability, including higher rates of the population living alone, disabilities, advanced age, and lack of health insurance coverage. Coolest areas corresponded to vegetated landscapes and urban water bodies. The urban HVI map will be useful to health officers, emergency preparedness personnel, the National Weather Service, and San Juan residents, as it helps to prepare for and to mitigate

  8. Marine Biodiversity in Juan Fernández and Desventuradas Islands, Chile: Global Endemism Hotspots.

    Science.gov (United States)

    Friedlander, Alan M; Ballesteros, Enric; Caselle, Jennifer E; Gaymer, Carlos F; Palma, Alvaro T; Petit, Ignacio; Varas, Eduardo; Muñoz Wilson, Alex; Sala, Enric

    2016-01-01

    The Juan Fernández and Desventuradas islands are among the few oceanic islands belonging to Chile. They possess a unique mix of tropical, subtropical, and temperate marine species, and although close to continental South America, elements of the biota have greater affinities with the central and south Pacific owing to the Humboldt Current, which creates a strong biogeographic barrier between these islands and the continent. The Juan Fernández Archipelago has ~700 people, with the major industry being the fishery for the endemic lobster, Jasus frontalis. The Desventuradas Islands are uninhabited except for a small Chilean military garrison on San Félix Island. We compared the marine biodiversity of these islands across multiple taxonomic groups. At San Ambrosio Island (SA), in Desventuradas, the laminarian kelp (Eisenia cokeri), which is limited to Desventuradas in Chile, accounted for >50% of the benthic cover at wave exposed areas, while more sheltered sites were dominated by sea urchin barrens. The benthos at Robinson Crusoe Island (RC), in the Juan Fernández Archipelago, comprised a diverse mix of macroalgae and invertebrates, a number of which are endemic to the region. The biomass of commercially targeted fishes was >2 times higher in remote sites around RC compared to sheltered locations closest to port, and overall biomass was 35% higher around SA compared to RC, likely reflecting fishing effects around RC. The number of endemic fish species was extremely high at both islands, with 87.5% of the species surveyed at RC and 72% at SA consisting of regional endemics. Remarkably, endemics accounted for 99% of the numerical abundance of fishes surveyed at RC and 96% at SA, which is the highest assemblage-level endemism known for any individual marine ecosystem on earth. Our results highlight the uniqueness and global significance of these biodiversity hotspots exposed to very different fishing pressures.

  9. Community-based enterprises and the commons: The case of San Juan Nuevo Parangaricutiro, Mexico

    Directory of Open Access Journals (Sweden)

    Alejandra Orozco-Quintero

    2009-09-01

    Full Text Available Commons scholarship has tended to focus on the administration and use of commons by individuals and households and less so on collective enterprises that extract, transform and market what they harvest from the commons. In this paper, we consider Nuevo San Juan, a Mexican case that is well known in the community forestry and commons literature. In San Juan, indigenous community members who hold the rights for the commons are also the members of the enterprise that transforms and markets goods from the commons. We argue that such a strategy is one way to confront internal and external pressures on a commons. We draw upon the transcripts of 40 interviews undertaken during 2006 which are analyzed using a framework developed from the social, community-based and indigenous enterprise literature. Our goal was to utilize this framework to analyze the San Juan Forest Enterprise and understand its emergence and formation as a long-standing community-based enterprise that intersects with a commons, and thereby identify factors that increase chances of success for community enterprises. We found that by starting from the community-based and indigenous enterprise literature and using that literature to engage with thinking on commons, it was possible to consider the enterprise from the perspective of a regulatory framework rather than from the poles of dependency and modernization theories in which much commons work has been based. Enterprise and commons intersect when both are guided by core cultural values and the enterprise can become a new site for the creation of social and cultural cohesion. We also found that there were a number of necessary conditions for commons-based community-enterprises to retain internal and external legitimacy, namely: (1 leadership representative of the broad social mission rooted in the customary institutions, values and norms of the community; (2 accountability of enterprise leaders to the memberships they represent; and (3

  10. Las correcciones de Juan Rejano. En torno a Canciones de la paz (1955

    Directory of Open Access Journals (Sweden)

    Luján Atienza, Ángel Luis

    2016-06-01

    Full Text Available The aim of this paper is to explain the reasons for Juan Rejano to have removed his book Canciones de la paz (Songs for the Peace, 1955 from his first almost complete poems collection, Alas de tierra (Wings of Earth, 1975, and to modify it in his last «collected poems», La mirada del hombre (The Man’s Sight, 1978. In this paper, the circumstances under which the book was written will be analysed, these being linked to the World Movement for Peace and being deeply influenced by the P.C.E. (Spanish Communist Party exhortations. The study of textual variants, deleted poems and new assignments in finished poems reveals that Juan Rejano feels uncomfortable with the combative voice of this part of his work as the instrument to reveal the ugliest side in contemporary life. In addition, this book, intended to be written for children, does not fit in young readers literature.Este artículo trata de dar cuenta de las razones que llevaron a Juan Rejano a eliminar el libro Canciones de la paz (1955 de su primera gran recopilación, Alas de tierra (1975, y a modificarlo sustancialmente en su última suma poética, La mirada del hombre (1978. Con el análisis de las circunstancias en que nació el libro, vinculado al Movimiento Mundial por la Paz e influenciado por las consignas del PCE, y con el estudio de variantes, poemas eliminados y poemas reubicados, podemos aventurar que Rejano no se sentía cómodo con la parte más combativa del libro y con la que presentaba la faceta más sombría de la realidad contemporánea, además de que no cabía el adoctrinamiento en una escritura explícitamente dirigida a los niños.

  11. VITICULTURA Y POLÍTICA INTERNACIONAL: EL INTENTO DE REINCORPORAR A MENDOZA Y SAN JUAN A CHILE (1820-1835

    Directory of Open Access Journals (Sweden)

    PABLO LACOSTE

    2006-06-01

    Full Text Available Entre 1830 y 1835 algunos dirigentes de Mendoza y San Juan, provincias vitivinícolas, trataron de separarse de la República Argentina y regresar a Chile, tal como en la época colonial. Durante más de dos siglos, Mendoza y San Juan fueron parte del Corregimiento de Cuyo del Reino de Chile. Esta situación cambió desde 1776 con la creación del Virreinato del Río de la Plata. Después de la Revolución de 1810, Mendoza y San Juan formaron parte de la República Argentina. Pero algunos años después de la independencia, un grupo descontento con la acción y decisiones del gobierno de Buenos Aires, inició acciones orientadas a la secesión. Este artículo investiga las razones y motivaciones socioeconómicas de este grupoBetween 1830 and 1835 some leaders of Mendoza and San Juan, wine industry oriented provinces, attempted to withdrawal from Argentina Republic, and come back to Chile such as in colonial times. During more than two centuries, Mendoza and San Juan were members of Cuyo Province in Chile's Kingdom. This situation changed since 1776 because the creation of the River Plate Viceroyalty. After Revolution of 1810, Mendoza and San Juan took part of Argentina Republic. But some years after the independence, a group discontented with de action and decision of Buenos Aires government begun the action oriented to secession. This paper explores the socioeconomic reasons and motivation of this group

  12. Seguimiento de la construcción de edificio 43 viviendas Av/ Juan XXIII Valencia

    OpenAIRE

    BURGOS ROMERO, LUIS

    2011-01-01

    Convenios con empresa. La obra prevé la construcción de un edificio de 41 viviendas VP + 2 viviendas libres, constando de 2 plantas sótano para uso de aparcamiento y trasteros, planta baja con local comercial, 8 plantas con 5 viviendas por planta y una planta ático con 3 viviendas. La obra está en su fase de estructura, habiéndose realizado las plantas de sótano. Burgos Romero, L. (2011). Seguimiento de la construcción de edificio 43 viviendas Av/ Juan XXIII Valencia. http://hdl.handle.net...

  13. Tierra, mano de obra y violencia en el altiplano Guatemalteco: San Juan IXCOY

    OpenAIRE

    Mc Creery, David

    2012-01-01

    Introducción El monocultivo cafetalero había amenazado  la integridad de la agricultura campesina indígena como ningún otro cultivo anterior. A pesar de que el blanco inmediato de la ira indígena era el desventurado grupo de  habilitadores, el problema subyacente mas serio de la población indígena era el acceso y control de la tierra. Esta investigación se centrara en el  problema de la tierra, sobre todo en  San Juan Ixcoy como un ejemplo de esta lucha.

  14. Cuentos terroríficos del antiguo Juan Viñas: El Sitio.

    OpenAIRE

    Quesada Vargas, María

    2012-01-01

    Cuentos: Los compadres, Redención, Regreso iluminado, Auxilio divino, Alaridos inciertos, Comida mortífera, Terror sonoro, Enemigo feroz, La casona, A la tercera, Uno más, Contratiempo, Paseante del infierno, Doble identidad, Cuidado materno, Diablo honrado, Espantos espantados, Perdidas en el tiempo, Muchacho impío, Pelea fraternal, Negro sonriente, Persecución implacable, San Lucas, Ojo modernísimo, Pronóstico acertado Horror Stories from Old Juan Viñas: El Sitio

  15. Plan de mercadeo para el ramo de riesgos laborales en JuanSa Seguros

    OpenAIRE

    Jiménez Pizarro, Andrés Felipe; Tamayo Sandoval, Juan Carlos

    2016-01-01

    En este plan de mercadeo se realiza un análisis de la situación actual de la empresa JuanSa Seguros con alcance específico en el producto de riesgos laborales en el mercado corporativo del Valle del Cauca. Este análisis incorpora variables como los clientes, las capacidades actuales de la empresa, la competencia, los colaboradores y el contexto; a continuación se estudia el mercado con la información disponible por la empresa llegando a un segmento objetivo de 170 empresas.

  16. Violência e sociedade em Festa no Covil, de Juan Pablo Villalobos

    OpenAIRE

    Benatti, Andre Rezende; Universidade Federal do Rio de Janeiro - UFRJ

    2015-01-01

    O presente artigo tem como objetivo uma leitura da violência no romance Festa no Covil, do escritor mexicano Juan Pablo Villalobos. Abordaremos dentre outras questões como a violência se torna parte da rotina do pequeno narrador do romance, Tochtli, tornando-se não mais uma surpresa ou estranheza ou repulsa, mas algo corriqueiro que o acompanha diariamente. No romance, Tochtli é o herdeiro de um cartel de narcotráfico e é criado em um meio no qual a violência está tão presente que o menino nã...

  17. Cruzando fronteras: los fenómenos transpositivos de Juan Moreira. Novela, teatro y cine

    Directory of Open Access Journals (Sweden)

    Lía Sabrina Noguera

    2015-12-01

    Full Text Available En este artículo nos proponemos analizar la transposición de Juan Moreira en la literatura, el drama y, especialmente, en el cine argentino a partir de la versión que Leonardo Favio realizó en 1972. Nuestro objetivo es dar cuenta de las variantes de significación que se producen de un texto a otro, no solo en lo referente a lo propio del fenómeno transpositivo sino, y en especial, al modo en el que el contexto de producción resignifica los materiales discursivos y propone nuevos mitos sobre este héroe popular.

  18. El pan del misterio: interpretación socio-retórica de Juan 6

    Directory of Open Access Journals (Sweden)

    Juan Esteban Londoño

    2017-01-01

    Full Text Available Este artículo estudia el capítulo 6 del Evangelio de Juan desde una perspectiva socio-retórica. Enfatiza las estrategias de persuasión que aparecen en el texto para convencer a la audiencia de una alta cristología, y destaca los sentidos profundos de las palabras simbólicas, como también los valores y significados de un mundo social y cultural en conflicto con la sinagoga y las iglesias judeo-cristianas.

  19. The symbolic surrealistic image in the poetry of Juan Eduardo Cirlot

    OpenAIRE

    2010-01-01

    Este artigo procura estudar as imagens surrealistas e a sua polivalência na poesia de Juan Eduardo Cirlot, um dos membros do grupo catalão Dau al Set e um dos mais interessantes críticos de arte em Espanha na segunda metade do século XX. Considerando que as imagens criam a sua própria realidade e exprimem uma necessidade espiritual de viver nessa realidade criada, o nosso propósito é o de compreender, através da profunda afinidade com o surrealismo onírico, a obsessão recorrent...

  20. La estela de Orfeo : poética de la trascendencia en Juan Eduardo Cirlot

    OpenAIRE

    2007-01-01

    El presente trabajo explora la figura de Orfeo y la corriente del orfismo con la finalidad de establecer una nueva interpretación del mito a nivel psicológico, histórico y filosófico. El resultado pretende servir de base para el estudio de la poesía en poetas contemporáneos que aluden a dicho mito, que lo reescriben para crear su propia versión o bien que lo utilizan de herramienta para elaborar su poética personal. En este caso se toma como ejemplo la obra del poeta español Juan Eduardo Cirl...

  1. La huella de lo sagrado en la poesía de Juan Eduardo Cirlot

    OpenAIRE

    2012-01-01

    Poeta singular e iconoclasta, Juan Eduardo Cirlot (1916-1973) erigió con su poemario Bronwyn uno de los universos imaginarios más vastos, sugerentes y atractivos realizados en castellano a lo largo del siglo dejado atrás. Interesado por el serialismo musical, la cábala, el simbolismo y la práctica totalidad de corrientes artísticas de la antigüedad y modernas, Cirlot tomaría motivos de todos estos ámbitos para hacerlos confluir con sus propias búsquedas espirituales en su última etapa creativ...

  2. [Dr. Juan Ramon Beltran and his contribution to the School of Dentistry].

    Science.gov (United States)

    Zarranz, A

    1999-01-01

    He was in charge of the course of Legal Dentistry at the School of Dentistry from 1929 through 1932. He prepared the study program for this subject, basing it on the experience he had gained as professor in Legal Medicine at the Faculty of Medical Sciences in Buenos Aires. He published the book "Medicina Legal para la ensenanza de la Odontologia Legal y Social" (1932), and its second edition included an important contribution made by Dr. Juan Ubaldo Carrea, Main Professor of Orthodontics with Legal Dentistry at this school

  3. Aspectos do real maravilhoso no romance Pedro Páramo, de Juan Rulfo

    OpenAIRE

    2015-01-01

    La novela elegida para el presente análisis es Pedro Páramo, del escritor mexicano Juan Rulfo, publicada en 1955. La obra de Rulfo es capaz de suscitar diversas lecturas, tanto de los aspectos de la vida social como de la individual, concretizándose como el espacio de un nuevo lenguaje. En ese sentido, lo que se propone es un abordaje de la relación entre la realidad y el mundo ficcional, la memoria y el elemento sobrenatural en el desarrollo de la narrativa. De este modo, el objetivo de este...

  4. Rasgos significativos de la oralidad en la narrativa breve hispanoamericana: Juan Rulfo

    OpenAIRE

    2015-01-01

    El presente trabajo se inscribe dentro de un amplio campo de estudio dedicado al análisis de la oralidad fingida en la literatura y su principal objetivo es ofrecer una nueva perspectiva posible acerca del estudio de las técnicas narrativas utilizadas en la redacción de los cuentos del volumen de Juan Rulfo El Llano en llamas. La propuesta central intentará mostrar que la oralidad del lenguaje empleado en los relatos no reside exclusivamente en el uso de las múltiples estrategias que permiten...

  5. Diego Velázquez y la pintura oblicua de Juan Caramuel

    OpenAIRE

    Marías, Fernando

    2008-01-01

    La recuperación de un texto olvidado de Juan Caramuel de Lobkowitz, sobre el arte de la pintura y el retrato de Inocencio X de Diego Velázquez, permite el análisis de algunas de sus ideas sobre la pintura antigua y moderna, en relación con el debate contemporáneo entre rubensistas y poussinistas, y sobre el arte de Velázquez, en relación con sus propias teorías sobre la arquitectura oblicua y la necesaria adecuación entre la forma y la estructura significante de lo representado ...

  6. Cuentos terroríficos del antiguo Juan Viñas: El Sitio.

    OpenAIRE

    Quesada Vargas, María

    2012-01-01

    Horror Stories from Old Juan Viñas: El Sitio Cuentos: Los compadres, Redención, Regreso iluminado, Auxilio divino, Alaridos inciertos, Comida mortífera, Terror sonoro, Enemigo feroz, La casona, A la tercera, Uno más, Contratiempo, Paseante del infierno, Doble identidad, Cuidado materno, Diablo honrado, Espantos espantados, Perdidas en el tiempo, Muchacho impío, Pelea fraternal, Negro sonriente, Persecución implacable, San Lucas, Ojo modernísimo, Pronóstico acertado

  7. A EMERGÊNCIA DA ENTIDADE DON JUAN NA OBRA DE MILAN KUNDERA

    Directory of Open Access Journals (Sweden)

    Wilton Barroso*

    2010-12-01

    Full Text Available Este estudo tem por objetivo uma análise acerca do comportamento libertino1 dos don Juans2 kunderianos. De posse da compreensão do percurso dessa personagem na historiografia literária, objetiva-se uma comparação analítica dessa figura com as composições de Milan Kundera, tendo como centro de observação os seus universos objetivos e subjetivos de onde se acredita, emanam grandes temas vividos pela humanidade nos tempos atuais. PALAVRAS-CHAVE: Donjuanismo; Pós-modernidade; Subjetividade.

  8. El De Clausura Monialium de Galarza en el Don Juan de Azorín

    Directory of Open Access Journals (Sweden)

    Mañas Núñez, Manuel

    2013-06-01

    Full Text Available Este artículo estudia la influencia de la obra De clausura monialium controversia de Pedro García de Galarza en la novela Don Juan de Azorín. Se concluye que el Obispo Don García de Illán es el Obispo Don García de Galarza y que la ciudad donde ocurre el suceso de las monjas rebeldes es Cáceres (Diócesis de Coria-Cáceres.

  9. Entre arrumacos y arañazos: La gata revolcada de Juan José Barrientos

    OpenAIRE

    Andreu, Jean

    2015-01-01

    En materia de crítica literariael enfoque biográfico puede contribuira la comprensión de un texto difícil.QUI XIALONG, Red Mandarin Dress, 2006 Juan José Barrientos es un crítico literario extraordinario. Tal como suena. O por lo menos insólito. La desprocupación teórica con que lleva su argumentación, la erudición de sus muchas y raras lecturas y referencias, su manera de escribir tan agradable, a la vez sencilla e incisiva, hacen que no veo a otro semejante en nuestro prolijo gremio de los ...

  10. La Section d´Or y Juan Gris: Un camino hacia un cubismo espiritual

    OpenAIRE

    2016-01-01

    [ES] El artista de origen madrileño Juan Gris tuvo la oportunidad de ser testigo directo de la gestación del cubismo a raíz de su estancia en 1906 en la capital francesa. A partir de aquí, comienza a relacionarse con distintos pintores y críticos vanguardistas, que le ofertarán nuevas posibilidades aperturistas para su posterior involucración cubista. El año 1912 marca su definitiva integración bajo la estela pictórica del cubismo sintético, destacando igualmente su relación con el g...

  11. Una relectura de De insulis oceanis de Juan López de Palacios Rubios

    OpenAIRE

    Díaz Ceballos, Jorge

    2012-01-01

    RESUMEN. El tratado De insulis oceanis, escrito por Juan López de Palacios Rubios tras la Junta de Burgos de 1511, contribuyó a reforzar con argumentos jurídico-teológicos los títulos justificativos de la presencia castellana en las Indias. Palacios Rubios escribe su tratado en un momento decisivo de la colonización del Nuevo Mundo y su trabajo contribuiría a definir de qué manera se llevaron a cabo las conquistas inmediatamente posteriores. En el presente texto se plantea el anál...

  12. Regional Studies Program. Biological aspects of surface coal mine reclamation, Black Mesa and San Juan Basin

    Energy Technology Data Exchange (ETDEWEB)

    Green, B.B.

    1977-08-01

    This report discusses case study data on surface mine reclamation accumulated at selected mines in the Southwest, specifically the Black Mesa Mine in Arizona and the Navajo Mine located south of Fruitland, New Mexico, in the San Juan Basin. Experimental results have made it apparent that reclamation of disturbed lands cannot begin and end with planting seed. The report therefore considers hydrologic, edaphic, and biotic factors, as well as land use and management, as important aspects of land reclamation. Recommendations are made to initiate, broaden, and intensify research studies in plant succession, productivity, species selection, and attendant soil characteristics, along with grazing practices and land use potential.

  13. Juan de Pitano y Bolívar, platero vitoriano del siglo XVI

    OpenAIRE

    Martín Vaquero, Rosa

    1997-01-01

    Juan de Pitano y Bolívar is one of the most important silversmiths of the second half of the 16th century in Vitoria. He was the successor to one of Vitoria's oldest silversmiths which had its origins in the previous century. Here we provide a biography of the man and present ten pieces of his work in which we are able to appreciated their quality together with the artistic techniques employed. All of the pieces bear his mark: PI/TANO. He was a master of his art and participated in all three ...

  14. The constitution of marginalized identity in Signs of identity by Juan Goytisolo

    OpenAIRE

    Jelica Veljović

    2014-01-01

    The main objective of the article is to investigate the modes of constitution of a marginalized and exiled identity in the novel Signs of identity by Juan Goytisolo. It is categorised as a “testimony and postwar novel”, and the subjects’ constant search of themselves, of their Self and all the elements that constitute their identity is central to the work. The novel is set in the postwar socio-historical period and the protagonist – Alvaro Mendiola – searches for his identity in his childhood...

  15. Cuentos terroríficos del antiguo Juan Viñas: Infiernillo (Santa Marta)

    OpenAIRE

    Quesada Vargas, María

    2012-01-01

    Horror Stories from Old Juan Viñas: Little Hell (Santa Marta) Cuentos: Mirada perdida, Caricia yerta, Llanto angelical, Lamentos, Resplandor mañanero, Sonrisa helada, La luz azul, Nuevas amistades, Castigo divino, Ave sorprendente, Extraña fortuna, Oración milagrosa, Pequeña compañía , El deshaucio, En carne viva, Gratitud constante, De luto, Lucecita falsa, Malestar repentino, Sitio encantado, Pan incomible, ¿La vecina?, Conducta dudosa, Enmontañado, Uñas celosas, Risas malévolas, Ruta an...

  16. Cerebro y metáfora en la novela "Pedro Páramo", de Juan Rulfo

    Directory of Open Access Journals (Sweden)

    CECILIA EDILMA DÍAZ DÍAZ

    2010-01-01

    Full Text Available Se trata de un avance de investigación que aplica la teoría neurolingüística de Omar Parra Rozo para leer y comprender la metáfora en un texto literario. Se toma como objeto de estudio la novela "Pedro Páramo" de Juan Rulfo, específicamente los fragmentos que hablan acerca de la imagen compleja (religión-poder y muerte. El ejercicio de investigación alcanzó a establecer la relación que existe entre imagen- cerebro y metáfora, en el discurso de esta novela.

  17. Del pueblo elegido y el maná escondido. La minera en San Juan (Argentina)

    OpenAIRE

    Mirta Alejandra Antonelli

    2016-01-01

    Este artículo aborda el desastre ambiental provocado en la mina Veladero, que explota la canadiense Barrick Gold, como implosión del núcleo duro del modelo de la megaminería metalífera en Argentina, que ha tenido como «religión y culto» a la cordillerana provincia de San Juan. Se analiza aquí la narrativa promesante que sostienen los discursos dominantes, aquellos que conforman un dispositivo de control de mundos posibles para la generación de creencias, ideas, valores. Desde una perspectiva ...

  18. San Juan single-well seismic data analysis and modeling study

    Energy Technology Data Exchange (ETDEWEB)

    Daley, Tom; Wu, C.; Harris, J.M.; Daley, T.M.; Majer, E.L.

    2004-02-26

    The authors analyze single-well seismic data from the San Juan basin in Northwest New Mexico. The consistently observable events are tube-waves: direct, reflected and multiple tube-waves can be explained by the formation properties and survey geometry except for an anomalous zone with low velocity, high amplitude and horizontal polarization. To aid the data analysis, forward modeling using a variable-grid finite-difference parallel code is performed. The numerical result confirms the identified events in the field observations.

  19. Pyroclastic eruptions from Axial caldera, Juan de Fuca Ridge, NE Pacific Ocean

    Energy Technology Data Exchange (ETDEWEB)

    Helo, Christoph; Stix, John [Department of Earth and Planetary Sciences, McGill University, 3450 University Street, Montreal, Quebec H3A 2A7 (Canada); Clague, Dave A [Monterey Bay Aquarium Research Institute 7700 Sandholdt Road, Moss Landing, CA 95039-9644 (United States)

    2008-10-01

    Unconsolidated volcaniclastic glass deposits on the flanks of Axial Seamount, a caldera system situated on the Juan de Fuca Ridge in the NE Pacific Ocean, demonstrate the occurrence of explosive events, in addition to effusive activity. The variety of produced glass fragments ranges from various angular forms to thin deep-sea limu o Pele, with dominantly moderately fractionated to occasionally primitive MOR basalt composition. A model involving the collapse of a magmatic foam layer may account for the observed spectrum of glass fragments.

  20. Hydraulic fracturing of tight CBM reservoirs in the San Juan Basin, NM

    Energy Technology Data Exchange (ETDEWEB)

    Goodwin, R. [Burlington Resources, Calgary, AB (Canada)

    2001-07-01

    Stimulation techniques, reservoir size and effects of stimulation, and regulatory compliance are described. The gas content, thickness, and pressure characteristics of San Juan Fruitland coal are considered. Cavitation, no proppant injection, sand-laden fluids, foamed fluids, sand control additives, coiled tubing fracturing, and breakdown have been tried, and better stimulation techniques are being pursued. Reduced pad size and minimal new road development to prevent surface damage, disposal of stimulation fluids in disposal wells, and surface casing and zonal isolation to protect surface waters has minimized damage to the environment during fracturing and disposal. 3 figs.

  1. UN VIAJE DE IDA Y VUELTA A MÉXICO: EL TESTIGO DE JUAN VILLORO.

    OpenAIRE

    Alejandro, Hermosilla Sánchez

    2010-01-01

    En el presente artículo nos proponemos llevar a cabo un análisis de la novela del mexicano Juan Villoro, El testigo. Este análisis nos permitirá no sólo conocer mejor cómo y de qué manera aparece México en esta y otras novelas de Villoro, sino, además y más importante aún, la importancia de López Velarde y la ironía en su obra así como su significado final.

  2. “Ensayar: leer en compañía”: la biblioteca argentina de Juan Villoro

    OpenAIRE

    Remón-Raillard, Margarita

    2015-01-01

    El escritor mexicano Juan Villoro, en su faceta de crítico literario, ha teorizado sobre géneros o prácticas liminares como el ensayo, la crónica o la traducción. Una parte importante de sus ensayos se hallan reunidos en los volúmenes Efectos personales y De eso se trata, en los que nos presenta su lectura de una serie de obras de la literatura hispanoamericana, anglosajona y europea.A partir de la reflexión de Pierre Bayard sobre la noción de biblioteca y sus distintas acepciones (la bibliot...

  3. Sismicidad superficial entre 1995 y 2010 en la zona norte de la Precordillera de San Juan

    OpenAIRE

    Rivas, C.; Bilbao,I.; Sánchez, G.; M. Araujo; Aguil,B.; Alvarado, P.

    2017-01-01

    Los avances en el campo de la Sismología, tanto en las técnicas de procesamiento y análisis de datos como en la parte instrumental y en sistemas informáticos, han permitido extraer mayor información de los registros sísmicos y refinar los parámetros que caracterizan la fuente sísmica y la estructura del subsuelo. Para estudiar la sismicidad cortical más cercana a la represa Cuesta del Viento situada en la zona norte de la Precordillera de San Juan, se seleccionaron sismos registrados por l...

  4. Santana Pérez, Juan Manuel, La Historia contraataca, Fundación Buria

    OpenAIRE

    Castelao, Ofelia Rey

    2015-01-01

    El nuevo libro de Juan Manuel Santana retoma algunos de los temas abordados por él en publicaciones anteriores, pero apuesta por una renovación de sus propios planteamientos a la luz de la rápida evolución de la disciplina histórica y de la multiplicación de sus practicantes, un proceso acelerado en el que a simple vista se detecta un empobrecimiento de la reflexión, de la crítica y, en general, de las bases teóricas en las que la historia debe apoyarse. El libro no se presenta como un manual...

  5. ¿Una leyenda ? Juan March (1880-1962)

    OpenAIRE

    Mercedes Cabrera

    2012-01-01

    La primera vez que escribí sobre Juan March Ordinas (1880-1962) fue para intervenir en un seminario sobre “Escándalos políticos y responsabilidad pública en la España contemporánea”, en abril del 2000. Conté el proceso que siguió contra March la Comisión de Responsabilidades de las Cortes Constituyentes de la República. March era diputado y el suplicatorio correspondiente se tramitó en medio de denuncias de corrupción y soborno contra varios diputados radicales. La Comisión le encarceló y le ...

  6. Un acercamiento a la literatura de horror en "El guardagujas" de Juan José Arreola

    OpenAIRE

    Alvaro A. Rodríguez S.

    2000-01-01

    P retender relacionar "El guardagujas" con la literatura de horror resulta dificultoso si se repasan algunos de los estudios yue se han elaborado sobre este cuento. Por ejemplo, el de Thomas O. Bcntc," 'El guardagujas' de Juan José Arreola: ¿Sátira política o explicación metafísica?", se aleja radicalmente no sólo de las explicaciones metafísicas a la obra del mexicano, sino también de sus supuestos nexos con lo real- maravilloso, y afirma que "El guardagujas" es una directa y mordaz crícica ...

  7. PRELIMINARY GEOID MODEL IN SAN JUAN PROVINCE: A CASE STUDY IN THE ANDES

    DEFF Research Database (Denmark)

    Tocho, Claudia; Miranda, Silvia; Pacino, Maria Cristina

    2008-01-01

    A high-resolution and high-precision detailed gravimetric geoid has been computed for San Juan province in Argentina, ranging from 27 degrees S to 34 degrees S in latitude and 72 degrees W to 65 degrees W in longitude. The gravimetric geoid was calculated using the RTM method, a multiband spherical...... Stokes Fast Fourier Transformation, and the removerestore technique for the spherical harmonic reference field and the terrain. As an external evaluation, the gravimetric quasigeoid/geoid was compared to the geoid heights obtained from 90 GPS/levelling points available for the province. Finally, a GPS......-tailored local geoid, which fits the GPS observations, was computed....

  8. SOCIAL MANAGEMENT OF IRRIGATION WATER IN THE SAN JUAN EJIDO, URIREO, SALVATIERRA, GUANAJUATO

    Directory of Open Access Journals (Sweden)

    Julieta Aidee Díaz-Rosillo

    2011-09-01

    Full Text Available In irrigated agriculture, producers are responsible for the management and administration of multiple common resources, among which include land and water. These common resources are used jointly by the whole community and in the same way are removed, depending on the needs of each individual. In the case of well 15 in the Ejido San Juan, has been maintained to be administered only by users without needing them, so far, the involvement of people outside the community or any government body for best results.

  9. Reacciones adversas a medicamentos como causa de consulta en el servicio de emergencias del Hospital San Juan de Dios

    OpenAIRE

    Unfried Segura, Erika

    2008-01-01

    Objetivo: Estimar el número de reacciones adversas que son causa de consulta en el Servicio de Emergencias en el Hospital San Juan de Dios, San José, Costa Rica por un periodo de estudio de 31 días. Diseño: Estudio observacional prospectivo. Lugar: Servicio de Emergencias del Hospital San Juan de Dios. San José, Costa Rica. Pacientes: Pacientes = 12 años de edad que fueron diagnosticados y/o consultaron debido a una reacción adversa a medicamentos en el Servicio de Emergencias durante el peri...

  10. Juan Rejano en México: Los criterios del crítico literario

    Directory of Open Access Journals (Sweden)

    Olmedo Muñoz, Iliana

    2015-12-01

    Full Text Available Making use of the articles published by Juan Rejano in El Nacional, this paper defines Rejano’s criteria in order to make known the critical perspective of Spanish exiles about Mexican literature and to insist in the importance of a dialogue between Mexican and Exile intellectuals. Despite the registry of his column is wide, Rejano’s critique about Mexican narrative explains his tastes, his ideas of tradition and literary quality. Moreover, the specific case of Rejano demonstrates how Spanish exiles participated and exerted influence in Mexico’s cultural space.Este trabajo analiza la columna que Juan Rejano mantuvo durante décadas en el diario El Nacional como ejemplo de la crítica literaria que los exiliados españoles realizaron en México, un capítulo importante y poco estudiado del diálogo cultural entre intelectuales exiliados y mexicanos. Aunque el registro de la columna es muy amplio, examinar la labor de Rejano como crítico de narrativa mexicana revela sus filiaciones, su idea de tradición y de calidad literaria. Además descubre, desde el caso concreto de Rejano, cómo los exiliados participaron e influyeron en la conformación del medio cultural mexicano.

  11. Leonardo da Vinci’s anatomical drawings and Juan de Arfe y Villafañe

    Directory of Open Access Journals (Sweden)

    Röhrl, Boris

    2014-06-01

    Full Text Available The four volumes of the Varia commensuración para la escultura y architectura (1585–87 of the Spanish silversmith and sculptor Juan de Arfe y Villafañe represent an extensive compilation of Italian art techniques of the Renaissance. The sources for the Libro segundo, which includes systematic explanations on the theory of proportions, the skeleton and musculature have yet to be discovered. Its didactic and illustrative structure is unique in the context of sixteenth-century literature. The general layout and some details of the illustrations of the first three chapters seem to be derived from the Windsor convolute of anatomical drawings by Leonardo da Vinci.Los cuatros libros de Varia commensuración para la escultura y architectura (1585-1587 del platero y escultor Juan de Arfe y Villafañe representan un extenso compendio de las teorías y técnicas del Renacimiento italiano. Las fuentes del Libro segundo, que contiene explicaciones sistemáticas sobre la proporción de la figura humana, el esqueleto y la musculatura, aún no han sido descubiertas. La estructura didáctica y las ilustraciones son únicas en el contexto de la literatura del siglo XVI. El formato general y algunos detalles de las ilustraciones de los primeros tres capítulos parecen tener su origen en los dibujos anatómicos de Leonardo da Vinci conservados en el castillo de Windsor.

  12. El naturalista Alejando de Humboldt, Cavanilles y Juan Andrés

    Directory of Open Access Journals (Sweden)

    María-Rosario MARTÍ MARCO

    2009-11-01

    Full Text Available RESUMEN: Tras una breve introducción en la que se traza un paralelismo entre el erudito Juan Andrés y el naturalista Alejandro de Humboldt, se presenta una síntesis de la relevancia del viaje científico de Humboldt iniciado en España. Las referencias sobre la figura y trabajo de Humboldt y, en menor medida, sobre Cavanilles en la gran obra total de Andrés demuestran el vigor intelectual de éste, quien subraya muchos de los elementos fundamentales del legado humboldtiano.ABSTRACT: This paper begins with a brief introduction to the similarities between the scholar Juan Andrés and the naturalist Alexander von Humboldt and continues with a summary of the relevance of Humboldt’s scientific expedition which began in Spain. The references to Humboldt’s persona and endeavours, and to a lesser extent to Cavanilles, in all of Andrés’ great works demonstrate the intellectual ability of the author who pinpoints many of the fundamental aspects of Humboldt’s legacy.

  13. Imaging the Juan de Fuca plate beneath southern Oregon using teleseismic P wave residuals

    Science.gov (United States)

    Harris, R.A.; Iyer, H.M.; Dawson, P.B.

    1991-01-01

    Images the Juan de Fuca plate in southern Oregon using seismic tomography. P wave travel time residuals from a 366-km-long seismic array operated in southern Oregon in 1982 are inverted. The southeast striking array extended from the Coast ranges to the Modoc Plateau and crossed the High Cascades at Crater Lake, Oregon. Three features under the array were imaged: one high-velocity zone and two low-velocity zones. The high-velocity zone is 3-4% faster than the surrounding upper mantle. It dips steeply at 65?? to the east beneath the Cascade Range and extends down to at least 200 km. It is proposed that this high-velocity feature is subducted Juan de Fuca plate. Two low-velocity zones were also imaged, both of which are 3-4% slower than the surrounding earth structure. The southeastern low-velocity zone may be caused by partially molten crust underlying the Crater Lake volcano region. -from Authors

  14. Anastas o el origen de La Constitución y el compromiso de Juan Benet

    Directory of Open Access Journals (Sweden)

    Carrera Garrido, Miguel

    2009-12-01

    Full Text Available During the Spanish post-war period, there were many anti-Francoist playwrights who decided to disguise their works, so the censors would not notice their criticism of the system. Juan Benet, whose dramatic corpus is quite short, could be one of them, mainly because of his play Anastas or the Origins of the Constitution (1958. Our purpose is to find out if it actually follows the steps of the possibilist theatre carried out by Buero Vallejo and others, or if on the contrary, it avoids political commitment and like most of the works written by Benet, answers to strictly personal criteria, opposite to militancy and favourable to technical innovation.Durante la posguerra española, muchos dramaturgos antifranquistas optaron por disfrazar sus obras, de tal modo que sus críticas al sistema pasaran desapercibidas a la censura. Juan Benet, autor de un reducido corpus dramático, podría ser uno de ellos, sobre todo por su drama Anastas o el origen de la Constitución (1958. Nuestra intención consiste en dilucidar si, en efecto, se encuentra en la línea del teatro posibilista escrito por Buero Vallejo y otros o si, por el contrario, rehúye el compromiso político y, como la mayor parte de la literatura benetiana, responde a unos criterios estrictamente personales, opuestos a la militancia y favorables a la innovación técnica.

  15. El destierro infinito de Blanco White en la mirada de Juan Goytisolo

    Directory of Open Access Journals (Sweden)

    Durán López, Fernando

    2010-06-01

    Full Text Available In the years 1970-1972, the novelist Juan Goytisolo published several studies and translations on the work of José María Blanco White, which represent a key moment in the recovery of that forgotten writer for the modern Spanish culture. The argument made in this essay is that the consciously identifying strategy applied by Goytisolo on Blanco White, in order to define a Spanish intellectual tradition throughout the centuries, marked by exile and dissent, is in fact a deformation of the figure and thought of the writer from Seville. Blanco White’s reception in Spain, however, has been conditioned by that reading.En torno a los años 1970-1972 el novelista Juan Goytisolo publica varios estudios y traducciones sobre la obra de José María Blanco White, que suponen un momento clave en la recuperación de ese olvidado escritor para la moderna cultura española. La tesis formulada en el presente artículo es que la estrategia conscientemente identificadora que aplica Goytisolo sobre Blanco White, a fin de definir una tradición intelectual española a lo largo de los siglos marcada por el exilio y la disidencia, supone de hecho una deformación de la figura y el pensamiento del escritor sevillano. La recepción de Blanco White en España ha quedado, sin embargo, condicionada por esa lectura.

  16. Application of an area of review variance methodology to the San Juan Basin, New Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Dunn-Norman, S.; Warner, D.L.; Koederitz, L.F.; Laudon, R.C.

    1995-12-01

    When the Underground Injection Control (UIC) Regulations were promulgated in 1980, existing Class II Injection wells operating at the time were excluded from Area of Review (AOR) requirements. EPA has expressed its intent to revise the regulations to include the requirement for AOR`s for such wells, but it is expected that oil and gas producing states will be allowed to adopt a variance strategy for these wells. An AOR variance methodology has been developed under sponsorship of the American Petroleum Institute. The general concept of the variance methodology is a systematic evaluation of basic variance criteria that were agreed to by a Federal Advisory Committee. These criteria include absence of USDWs, lack of positive flow potential from the petroleum reservoir into the overlying USDWs, mitigating geological factors, and other evidence. The AOR variance methodology has been applied to oilfields in the San Juan Basin, New Mexico. This paper details results of these analyses, particularly with respect to the opportunity for variance for injection fields in the San Juan Basin.

  17. Geologic controls on open-hole cavity completions in the San Juan basin

    Energy Technology Data Exchange (ETDEWEB)

    Kelso, B.S. (Advanced Resources International, Inc., Lakewood, CO (United States))

    1994-04-01

    The success of open-cavity-completed wells is measured (1) by how well mechanically the cavity is created, which results in a higher degree of completion efficiency; and (2) by production success, whereby production rates are improved relative to other completion techniques. Open-hole cavitation is highly successful in the San Juan basin fairway, but the technique has not been successful in other areas. Outside the fairway, particularly to the south, operators have not achieved mechanical success with cavity completions. To explain the change in permeability across the southern fairway boundary, an hypothesis is proposed that involves the presence of a northwest-trending basement structure, such as a fault or fault zone. Episodic post-Fruitland movement on the northeastern limb of the structure could have enhanced the fracturing of the coal seams, resulting in higher permeabilities and the higher productivity levels of both cavity and fracture-stimulated wells. Based on the San Juan basin data reviewed, it appears that a combination of adequate permeability, overpressuring, and coal rank of high-volatile A bituminous are required for mechanical success of cavity completions. Although the level of permeability is not quantified, it is the author's opinion that at least 5 md is required. High production rates from cavity-completed wells are a function of better completion efficiency, whereby the wellbore is linked to favorable permeability settings. 5 refs., 10 figs.

  18. Sub-Seafloor Carbon Dioxide Storage Potential on the Juan de Fuca Plate, Western North America

    Energy Technology Data Exchange (ETDEWEB)

    Jerry Fairley; Robert Podgorney

    2012-11-01

    The Juan de Fuca plate, off the western coast of North America, has been suggested as a site for geological sequestration of waste carbon dioxide because of its many attractive characteristics (high permeability, large storage capacity, reactive rock types). Here we model CO2 injection into fractured basalts comprising the upper several hundred meters of the sub-seafloor basalt reservoir, overlain with low-permeability sediments and a large saline water column, to examine the feasibility of this reservoir for CO2 storage. Our simulations indicate that the sub-seafloor basalts of the Juan de Fuca plate may be an excellent CO2 storage candidate, as multiple trapping mechanisms (hydrodynamic, density inversions, and mineralization) act to keep the CO2 isolated from terrestrial environments. Questions remain about the lateral extent and connectivity of the high permeability basalts; however, the lack of wells or boreholes and thick sediment cover maximize storage potential while minimizing potential leakage pathways. Although promising, more study is needed to determine the economic viability of this option.

  19. Increasing coal absolute permeability in the San Juan basin Fruitland formation

    Energy Technology Data Exchange (ETDEWEB)

    Mavor, M.J.; Vaughn, J.E. [Society of Petroleum Engineers, Richardson, TX (United States)

    1998-10-01

    Recently measured data show that the absolute permeability of coal natural fracture is increasing significantly with continued gas production in the San Juan basin Fruitland formation. This phenomenon caused gas-production rates to be many times greater than expected from early production history. The phenomenon also caused producing bottomhole pressures to increase when gas rates were constant, opposite from that expected from conventional applications of Darcy`s law. The increase in absolute permeability caused by gas desorption has been measured on cores but, until recently, there was no verification that this phenomenon occurs in situ. Palmer and Mansoori (P & M) presented a new theory and showed how this theory matched gas- and water-production rates and estimated bottomhole-pressure data for a high-deliverability San Juan basin Fruitland formation coal-gas well. However, Palmer and Mansoor had no transient pressure data to support in-situ permeability changes. This paper documents data from drillstem tests and shut-in tests with analyses thereof and additional production-rate and pressure behaviors that support the P & M theory.

  20. CO{sub 2} sequestration in an unmineable coalbed - San Juan Basin, Colorado, USA

    Energy Technology Data Exchange (ETDEWEB)

    Erickson, D.; Jensen, J.R. [BP Amoco, Sunbury-on-Thames (United Kingdom)

    2001-07-01

    BP Amoco (BPA) is investigating the sequestration of approximately 464 tonnes per day of vented CO{sub 2} from gas processing plant by injecting the CO{sub 2} into a non-mineable natural gas producing coal in the Colorado portion of the San Juan Basin. Removed CO{sub 2} from this production will be re-injected into the coalbed for sequestration. BPA has concentrated efforts on utilizing nitrogen to enhance the recovery of methane from coals and currently operates the largest and most significant project of its kind. On a pilot basis, CO{sub 2} sequestration, as an enhanced recovery process for Fruitland coals, has also been undertaken in the San Juan Basin (SJB). The two gases behave differently in coals. CO{sub 2} is readily adsorbed on the coal and therefore displaces the methane whereas the nitrogen strips the methane from the coals by reducing the partial pressure of the methane. Injection, production and reservoir data will be used to evaluate the success of the CO{sub 2} sequestration. BPA will use its proprietary GCOMP Reservoir Model to investigate the flow and adsorption of CO{sub 2}, simulate the process, compare field results with model predictions, and adjust the model to better match actual field performance. Preliminary simulations indicate that some incremental methane production will be recovered, while sequestering nearly all of the injected CO{sub 2} in the coals seams. 3 figs.

  1. Progressive migration and anagenesis in Drimys confertifolia of the Juan Fernández Archipelago, Chile.

    Science.gov (United States)

    López-Sepúlveda, Patricio; Takayama, Koji; Greimler, Josef; Crawford, Daniel J; Peñailillo, Patricio; Baeza, Marcelo; Ruiz, Eduardo; Kohl, Gudrun; Tremetsberger, Karin; Gatica, Alejandro; Letelier, Luis; Novoa, Patricio; Novak, Johannes; Stuessy, Tod F

    2015-01-01

    A common mode of speciation in oceanic islands is by anagenesis, wherein an immigrant arrives and through time transforms by mutation, recombination, and drift into a morphologically and genetically distinct species, with the new species accumulating a high level of genetic diversity. We investigate speciation in Drimys confertifolia, endemic to the two major islands of the Juan Fernández Archipelago, Chile, to determine genetic consequences of anagenesis, to examine relationships among populations of D. confertifolia and the continental species D. winteri and D. andina, and to test probable migration routes between the major islands. Population genetic analyses were conducted using AFLPs and nuclear microsatellites of 421 individuals from 42 populations from the Juan Fernández islands and the continent. Drimys confertifolia shows a wide genetic variation within populations on both islands, and values of genetic diversity within populations are similar to those found within populations of the continental progenitor. The genetic results are compatible with the hypothesis of high levels of genetic variation accumulating within anagenetically derived species in oceanic islands, and with the concept of little or no geographical partitioning of this variation over the landscape. Analysis of the probability of migration within the archipelago confirms colonization from the older island, Robinson Crusoe, to the younger island Alejandro Selkirk.

  2. Dinosaur Footprints in Lower Cretaceous Beds in San Juan Raya, Southern Mexico and the Paleoenvironmental Implications

    Science.gov (United States)

    Aceves, D.

    2008-12-01

    Dinosaur footprints were traced at San Juan Raya, an important site in Mexico, a world fossil site. This site is found at South-west of the State of Puebla, within the Biosphere Reserve of Tehuacan-Cuitcatlán, to the southwest of the Tehuacan valley. These footprints were recorded by tracing them on transparent paper at Barranca Agua Nueva, at point 18°18.56´N 97°37´W. Using Jacob´s staff a stratigraphic register was generated from 50 m ascending and descending in stratigraphically direction from the bed where footprints were founded. Bivalbes, nerineas, shell fragments, and trigonias were founded in this sequence as well as cross bedding of clays and fine grain sand, some which display ripples. Fifty two footprints were recorded and five different tracks identified, observing two different sizes. The tracks of dinosaur footprints present the common Teropode ichnites. The succession where dinosaur footprints have been found, are interpreted as a peritidal environment. This investigation contributes to an eco-tourism project of San Juan Raya.

  3. La junta luz di Juan Gelman: un oratorio contro il silenzio

    Directory of Open Access Journals (Sweden)

    Arianna Fiore

    2014-12-01

    Full Text Available Juan Gelman is generally considered by critics to be one of the most important poets of modern Argentina. Born in Buenos Aires in 1930 from Russian-Jewish parents, he published his first collection of poems (Violín y otras cuestiones in 1956. He is a member of the generación del 60, made up of intellectuals influenced in their critical realism by the charisma of Raúl González Tuñón, forerunner of Argentina’s social and political poetry. This group was politically very active, as demonstrated by their experimental language and iconoclastic lyricism. Gelman’s later life was marked by his exile in the wake of the 1976 dictatorship, and by the desaparición of his son Marcelo Ariel, his daughter-in-law Claudia and his grand-daughter Andrea (born in prison. Since then, it was impossible for Juan Gelman to separate his personal plight from Argentina’s historical-political situation, and his poetry became more and more intimate and lyrical, marked by human drama. La junta luz, the author’s only theatre play, was written and published in this second phase.

  4. Juan Luis Villanueva仿真软件在S7-200 PLC教学中的应用%Application of Juan Luis Villanueva in the S7-200 PLC simulation software in Teaching

    Institute of Scientific and Technical Information of China (English)

    崔瀚

    2015-01-01

    In order to improve the classroom teaching effect of PLC,Juan Luis Villanueva simulation software used in daily teaching of S7-200PLC course.First of all Juan Luis Villanueva simulation software to do a simple introduction,then through the realization of four motors start and stop of the sequence of examples of the use of simulation software in the teaching process,and finally a brief description of the simulation software in the PLC teaching advantage.%为了改善PLC课堂的教学效果,将Juan Luis Villanueva仿真软件用于S7-200PLC课程的日常教学。首先对Juan Luis Villanueva仿真软件做了简单介绍,之后通过实现四台电机顺序启动同时停止的例子介绍了使用仿真软件的教学过程,最后简要说明了采用仿真软件在PLC教学中的优势。

  5. Arsenic contamination of natural waters in San Juan and La Pampa, Argentina.

    Science.gov (United States)

    O'Reilly, J; Watts, M J; Shaw, R A; Marcilla, A L; Ward, N I

    2010-12-01

    Arsenic (As) speciation in surface and groundwater from two provinces in Argentina (San Juan and La Pampa) was investigated using solid phase extraction (SPE) cartridge methodology with comparison to total arsenic concentrations. A third province, Río Negro, was used as a control to the study. Strong cation exchange (SCX) and strong anion exchange (SAX) cartridges were utilised in series for the separation and preservation of arsenite (As(III)), arsenate (As(V)), monomethylarsonic acid (MA(V)) and dimethylarsinic acid (DMA(V)). Samples were collected from a range of water outlets (rivers/streams, wells, untreated domestic taps, well water treatment works) to assess the relationship between total arsenic and arsenic species, water type and water parameters (pH, conductivity and total dissolved solids, TDS). Analysis of the waters for arsenic (total and species) was performed by inductively coupled plasma mass spectrometry (ICP-MS) in collision cell mode. Total arsenic concentrations in the surface and groundwater from Encon and the San José de Jáchal region of San Juan (north-west Argentina within the Cuyo region) ranged from 9 to 357 μg l(-1) As. Groundwater from Eduardo Castex (EC) and Ingeniero Luiggi (LU) in La Pampa (central Argentina within the Chaco-Pampean Plain) ranged from 3 to 1326 μg l(-1) As. The pH range for the provinces of San Juan (7.2-9.7) and La Pampa (7.0-9.9) are in agreement with other published literature. The highest total arsenic concentrations were found in La Pampa well waters (both rural farms and pre-treated urban sources), particularly where there was high pH (typically > 8.2), conductivity (>2,600 μS cm(-1)) and TDS (>1,400 mg l(-1)). Reverse osmosis (RO) treatment of well waters in La Pampa for domestic drinking water in EC and LU significantly reduced total arsenic concentrations from a range of 216-224 μg l(-1) As to 0.3-0.8 μg l(-1) As. Arsenic species for both provinces were predominantly As(III) and As(V). As

  6. Sequence Stratigraphy of the Dakota Sandstone, Eastern San Juan Basin, New Mexico, and its Relationship to Reservoir Compartmentalization

    Energy Technology Data Exchange (ETDEWEB)

    Varney, Peter J.

    2002-04-23

    This research established the Dakota-outcrop sequence stratigraphy in part of the eastern San Juan Basin, New Mexico, and relates reservoir quality lithologies in depositional sequences to structure and reservoir compartmentalization in the South Lindrith Field area. The result was a predictive tool that will help guide further exploration and development.

  7. El mayor laboratorio en física del mundo Juan Antonio Rubio, candidato a dirigir el CERN

    CERN Multimedia

    2007-01-01

    The spanish Government presented last Friday at the CERN Council Juan Antonio Rubio, as candidate for the post of Director General of CERN. It's the first time that the spanish Government proposes a candidate for the Direction of this international research center. (2/3 page)

  8. AgRISTARS: Renewable resources inventory. Land information support system implementation plan and schedule. [San Juan National Forest pilot test

    Science.gov (United States)

    Yao, S. S. (Principal Investigator)

    1981-01-01

    The planning and scheduling of the use of remote sensing and computer technology to support the land management planning effort at the national forests level are outlined. The task planning and system capability development were reviewed. A user evaluation is presented along with technological transfer methodology. A land management planning pilot test of the San Juan National Forest is discussed.

  9. Kant e o problema da coisa em si no Idealismo Alemão, de Juan Adolfo Bonaccini

    Directory of Open Access Journals (Sweden)

    Glenn W. Erickson

    2005-12-01

    Full Text Available Resenha do livro "Kant e o problema da coisa em si no Idealismo Alemão: sua atualidade e relevância para a compreensão do problema da Filosofia", de Juan Adolfo Bonaccini. Rio de Janeiro: Relume Dumará, 2003. 442 páginas [Coleção Metafísica 3].

  10. 78 FR 38359 - Approval of Record of Decision for Relocation of Cattle Point Road, San Juan Island National...

    Science.gov (United States)

    2013-06-26

    ... National Park Service Approval of Record of Decision for Relocation of Cattle Point Road, San Juan Island... approved a Record of Decision for the Final Environmental Impact Statement for the relocation of the Cattle... Historical Park will begin to implement design and initiate construction of the Cattle Point Road...

  11. Incorporating Ecosystem Services into Community-level Decision-Making: A San Juan, Puerto Rico Case Study

    Science.gov (United States)

    EPA’s Office of Research and Development’s Sustainable and Healthy Communities Research Program is developing tools and approaches to incorporate ecosystem goods and services concepts into community-level decision-making. The San Juan Community Study is one of a serie...

  12. Paradiplomacia en las relaciones Chileno-Argentinas : la integración desde Coquimbo y San Juan

    NARCIS (Netherlands)

    Alvarez, M.

    2016-01-01

    The dissertation studies how non-central governments inserted themselves in the integration process between Argentina and Chile. As a case study, the link between the Argentinean province of San Juan and the Chilean region of Coquimbo was addressed through an extensive literature review and more

  13. Drácula vs Don Juan: la seducción y la condena en el cine

    OpenAIRE

    2013-01-01

    La investigación se centra en el estudio comparativo de paralelismos y puntos en común en la psicología, iconografía y puesta en escena de los mitos de Drácula y Don Juan en el ámbito cinematográfico.

  14. 75 FR 70818 - Traffic Separation Schemes: In the Strait of Juan de Fuca and Its Approaches; in Puget Sound and...

    Science.gov (United States)

    2010-11-19

    ... sight-obscured, right-angle turn in the presence of strong currents and numerous small craft. 7... Fuca and Its Approaches; in Puget Sound and Its Approaches; and in Haro Strait, Boundary Pass, and the... Strait of Juan de Fuca and its approaches, in Puget Sound and its approaches, and in Haro Strait...

  15. Writing Virtue and Indigenous Rights: Juan Bautista De Pomar and the "Relación de Texcoco"

    Science.gov (United States)

    Espericueta, José

    2015-01-01

    In his "Relación de Texcoco," Juan Bautista de Pomar (c. 1535-90) takes a political and moral stance against Spanish colonialism in Texcoco and the entire viceroyalty of New Spain. Responding to the "Instrucción y memoria's" (1577) request for information about the history and cultural practices of local populations, Pomar…

  16. Geology, thermal maturation, and source rock geochemistry in a volcanic covered basin: San Juan sag, south-central Colorado

    Energy Technology Data Exchange (ETDEWEB)

    Gries, R.R. [Priority Oil & Gas, Denver, CO (United States); Clayton, J.L. [Geological Survey, Denver, CO (United States); Leonard, C. [Platte River Associates, Denver, CO (United States)

    1997-07-01

    The San Juan sag, concealed by the vast San Juan volcanic field of south-central Colorado, has only recently benefited from oil and gas wildcat drilling and evaluations. Sound geochemical analyses and maturation modeling are essential elements for successful exploration and development. Oil has been produced in minor quantities from an Oligocene sill in the Mancos Shale within the sag, and major oil and gas production occurs from stratigraphically equivalent rocks in the San Juan basin to the southwest and in the Denver basin to the northeast. The objectives of this study were to identify potential source rocks, assess thermal maturity, and determine hydrocarbon-source bed relationships. Source rocks are present in the San Juan sag in the upper and lower Mancos Shale (including the Niobrara Member), which consists of about 666 m (2184 ft) of marine shale with from 0.5 to 3.1 wt. % organic carbon. Pyrolysis yields (S{sub 1} + S{sub 2} = 2000-6000 ppm) and solvent extraction yields (1000-4000 ppm) indicate that some intervals within the Mancos Shale are good potential source rocks for oil, containing type II organic matter, according to Rock-Eval pyrolysis assay.

  17. 78 FR 34128 - Notice of Inventory Completion: U.S. Department of Agriculture, U.S. Forest Service, San Juan...

    Science.gov (United States)

    2013-06-06

    ...The U.S. Department of Agriculture (USDA), Forest Service, San Juan National Forest has completed an inventory of human remains, in consultation with the appropriate Indian tribes or Native Hawaiian organizations, and has determined that there is a cultural affiliation between the human remains and present-day Indian tribes or Native Hawaiian organizations. Lineal descendants or......

  18. Diversity and Abundance of Ammonia-Oxidizing Archaea in Hydrothermal Vent Chimneys of the Juan de Fuca Ridge▿ †

    Science.gov (United States)

    Wang, Shufang; Xiao, Xiang; Jiang, Lijing; Peng, Xiaotong; Zhou, Huaiyang; Meng, Jun; Wang, Fengping

    2009-01-01

    The abundance and diversity of archaeal ammonia monooxygenase subunit A (amoA) genes from hydrothermal vent chimneys at the Juan de Fuca Ridge were investigated. The majority of the retrieved archaeal amoA sequences exhibited identities of less than 95% to those in the GenBank database. Novel ammonia-oxidizing archaea may exist in the hydrothermal vent environments. PMID:19395559

  19. CBM in 3-D: coalbed methane multicomponent 3-D reservoir characterisation study, Cedar Hill Field, San Juan Basin, New Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Davis, T.; Shuck, E.; Benson, R. [Colorado School of Mines, Golden, CO (United States). Dept. of Geophysics

    1995-10-01

    The article explains how 3-D multicomponent seismic surveys could substantially improve the production and development of fractured coalbed methane reservoirs. The technique has been used by Northern Geophysical for the detection of geological faults and zones of enhanced fracture permeability proximal to the fault in the western side of the Cedar Hill field in San Juan Basin, NM, USA. 3 figs.

  20. 33 CFR 161.55 - Vessel Traffic Service Puget Sound and the Cooperative Vessel Traffic Service for the Juan de...

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Vessel Traffic Service Puget Sound and the Cooperative Vessel Traffic Service for the Juan de Fuca Region. 161.55 Section 161.55 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY VESSEL TRAFFIC MANAGEMENT...

  1. 78 FR 34125 - Notice of Inventory Completion: U.S. Department of Agriculture, Forest Service, San Juan National...

    Science.gov (United States)

    2013-06-06

    ...The U.S. Department of Agriculture (USDA), Forest Service, San Juan National Forest has completed an inventory of human remains and associated funerary objects, in consultation with the appropriate Indian tribes or Native Hawaiian organizations, and has determined that there is a cultural affiliation between the human remains and associated funerary objects and present-day Indian tribes or......

  2. Letizia Gaeta, Juan de Borgoña e gli altri. Relazioni artistiche tra Italia e Spagna nel ‘400

    Directory of Open Access Journals (Sweden)

    Leonardo D'Agostino

    2014-07-01

    Full Text Available Recensione / Book review Gaeta L. (2012, Juan de Borgoña e gli altri. Relazioni artistiche tra Italia e Spagna nel ‘400, Galatina: Mario Congedo Editore («Saggi e testi. Collana diretta da Lucio Galante», 174 pp.

  3. Paradiplomacia en las relaciones Chileno-Argentinas : la integración desde Coquimbo y San Juan

    NARCIS (Netherlands)

    Alvarez, M.

    2016-01-01

    The dissertation studies how non-central governments inserted themselves in the integration process between Argentina and Chile. As a case study, the link between the Argentinean province of San Juan and the Chilean region of Coquimbo was addressed through an extensive literature review and more tha

  4. Tectonic geomorphology and paleoseismology of strike-slip faults in Jamaica: Implications for distribution of strain and seismic hazard along the southern edge of the Gonave microplate

    Science.gov (United States)

    Koehler, R. D.; Mann, P.; Brown, L. A.

    2009-12-01

    The east-west, left lateral strike-slip fault system forming the southern edge of the Gonave microplate crosses the110-km-long and 70-km-wide island of Jamaica. GPS measurements in the northeastern Caribbean are supportive of the microplate interpretation and indicate that ~ half of the Caribbean-North America left-lateral plate motion (8-14 mm/yr) is carried by the Plantain Garden (PGFZ) and associated faults in Jamaica. We performed Neotectonic mapping of the Plantain Garden fault along the southern rangefront of the Blue Mountains and conducted a paleoseismic study of the fault at Morant River. Between Holland Bay and Morant River, the fault is characterized by a steep, faceted, linear mountain front, prominent linear valleys and depressions, shutter ridges, and springs. At the eastern end of the island, the PGFZ is characterized by a left-stepping fault geometry that includes a major, active hot spring. The river cut exposure at Morant River exposes a 1.5-m-wide, sub-vertical fault zone juxtaposing sheared alluvium and faulted Cretaceous basement rocks. This section is overlain by an, unfaulted 3-m-thick fluvial terrace inset into a late Pleistocene terrace that is culturally modified. Upward fault terminations indicate the occurrence of three paleoearthquakes that occurred prior to deposition of the flat lying inset terrace around 341-628 cal yr BP. At this time, our radiocarbon results suggest that we can rule out the PGFZ as the source of the 1907 Kingston earthquake 102 years ago, as well as, the 1692 event that destroyed Port Royal 317 years ago and produced a major landslide at Yallahs. Pending OSL ages will constrain the age of the penultimate and most recent ruptures. Gently to steeply dipping rocks as young as Pliocene exposed in roadcuts within the low coastal hills south of and parallel to the Plantain Garden fault may indicate active folding and blind thrust faulting. These structures are poorly characterized and may accommodate an unknown amount of

  5. Juan Ruiz, Arcipreste de Hita, y Don Juan Manuel, Infante de Castilla : dos miradas para un mundo en crisis o la literatura como herramienta

    Directory of Open Access Journals (Sweden)

    Matías Escalera Cordero

    2007-12-01

    Full Text Available Hace unos años -en el cuarto número de Verba Hispanica-, en un trabajo titulado Juan Ruiz, un hombre de nuestro tiempo -fundamentado en lo sustancial por las lecturas que del Libro de buen amor, y de la figura de su autor, habían hecho Américo Castro y Jullio Rodríguez Puértolas -, comprobamos cómo la premeditada -y, en absoluto, casualcomplejidad interna del Libro se cimienta no sólo en la diversidad de los materiales que contiene y lo construyen, sino, sobre todo, en la unidad de sentido, que podríamos nombrar proto/novelesca, del conjunto entero: articulada, primero, en tomo de la "ficción realista" -psicológica- del yo literario que los informa; en la lógica –intensamente dialéctica- que los organiza; y, finalmente, en la radical ironía mediante la que el autor desentraña y desactiva todos y cada uno de los -engañosos- discursos artísticos, religiosos e ideológicos dominantes en su tiempo; especialmente, el cortesano, el clerical y el monetario.

  6. Estudio de las conductas prosociales en niños de San Juan de Pasto/ Prosocial Behaviors Study in Children San Juan de Pasto

    Directory of Open Access Journals (Sweden)

    Érika Alexandra Vásquez Arteaga

    2017-06-01

    Full Text Available Objetivo: El presente artículo es el resultado de una investigación finalizada desarrollada en tres escuelas públicas de San Juan de Pasto. El objetivo general se dirigió a develar las manifestaciones de las conductas prosociales de los niños para el diseño e implementación de una estrategia psicopedagógica. Método: La metodología se abordó desde el paradigma cualitativo, con un enfoque crítico social, de tipo investigación-acción. El presente artículo corresponde a la fase hermenéutica de la investigación en donde se emplearon como técnicas de recolección de información: narrativas, entrevistas focalizadas, observaciones participantes y sociodramas. Resultados: Los resultados indicaron que la empatía puede ser vista como una señal de debilidad o como una constante necesaria de interacción; en las conductas de ayuda hay manifestaciones principalmente no altruistas y la cooperación se presenta de manera intermitente según los estímulos ambientales.

  7. Juan C

    African Journals Online (AJOL)

    Information Technology

    John Locke, Gottfried Wilhelm Leibniz, George Berkeley, Immanuel Kant, John. Stuart Mill ... for ages (e.g. the nature of knowledge and knowledge acquisition). ... essence in the empirical sciences; and nominal and real definitions — essential.

  8. Juan Cruz

    OpenAIRE

    Cruz, Juan

    2013-01-01

    Prof. Cruz’s practice-based research has regularly concerned itself with text and translation as a metaphor for visual representation, and as an embodied and performative process. He has worked in a range of media including performance, video and installation, and exhibited his work widely since 1998 across Europe, Australia and Japan in institutions such as the Witte de With, Rotterdam, The Melbourne Festival, Serralves Foundation Porto, Camden Arts Centre and the South London Gallery. H...

  9. A simple high-throughput method for determination of antiepileptic analogues of γ-aminobutyric acid in pharmaceutical dosage forms using microplate fluorescence reader.

    Science.gov (United States)

    Martinc, Boštjan; Vovk, Tomaž

    2013-01-01

    Pregabalin (PGB), gabapentin (GBP), and vigabatrin (VGB) are structural analogues of γ-aminobutyric acid used for the treatment of different forms of epilepsy. Their analytical determination is challenging since these molecules have no significant UV or visible absorption. Several derivatization methods have been developed and used for their determination in bulk or pharmaceutical dosage forms. We aimed to develop a high- throughput method using a microplate reader with fluorescence detection and simple derivatization with fluorescamine. Obtained method involves derivatization step of only 5 min at room temperature and simultaneous measurements of 96 samples (λex 395, λem 476 nm) thus rendering excellent high-throughput analysis. The method was found to be linear with r²>0.998 across investigated analytical ranges of 0.75 to 30.0 µg/mL for PGB, 2.00 to 80.0 µg/mL for GBP, and 1.50 to 60.0 µg/mL for VGB. Intraday and interday precision values did not exceed 4.93%. The accuracy was ranging between 96.6 to 103.5%. The method was also found to be specific since used excipients did not interfere with the method. The robustness study showed that derivatization procedure is more robust than spectrofluorimetric conditions. The developed high-throughput method was successfully applied for determination of drug content and dissolution profiles in pharmaceutical dosage forms of studied antiepileptic drugs.

  10. Duration of plant damage by host larvae affects attraction of two parasitoid species (Microplitis croceipes and Cotesia marginiventris) to cotton: implications for interspecific competition.

    Science.gov (United States)

    Morawo, Tolulope; Fadamiro, Henry

    2014-12-01

    Volatile organic compounds (VOCs) released by herbivore-damaged plants can guide parasitoids to their hosts. The quantity and quality of VOC blends emitted by plants may be affected by the duration of plant damage by herbivores, which could have potential ramifications on the recruitment of competing parasitoids. We used two parasitoid species, Microplitis croceipes and Cotesia marginiventris (Hymenoptera: Braconidae), to address the question of whether duration of plant damage affects parasitoid use of plant VOCs for host location. Both wasp species are larval endoparasitoids of Heliothis virescens (Lepidoptera: Noctuidae), an important pest of cotton. Attraction of the two parasitoid species to odors emitted by undamaged (UD), fresh (6 h infestation) damage (FD), and old (24 h infestation) damage (OD) cotton plants infested by H. virescens larvae was investigated using a headspace volatile collection system coupled with four-choice olfactometer bioassay. Both sexes of M. croceipes showed a preference for FD- and OD-plant odors over UD-plants. On the other hand, more C. marginiventris females were attracted to UD- and FD-plants than to OD-plants. GC/MS analyses showed qualitative and quantitative differences in the VOC profiles of UD, FD, and OD-plants, which may explain the observed preferences of the parasitoids. These results suggest a temporal partitioning in the recruitment of M. croceipes and C. marginiventris to H. virescens-damaged cotton, and may have potential implications for interspecific competition between the two parasitoid species.

  11. Insecticidal Effect of Chrysanthemum coronarium L. Flowers on the Pest Spodoptera littoralis Boisd and its Parasitoid Microplitis rufiventris Kok. with Identifying the Chemical Composition

    Science.gov (United States)

    Shonouda, Mourad L.; Osman, Salah; Salama, Osama; Ayoub, Amal

    The flower extract of Chrysanthemum coronarium L. and their fractions have shown insecticidal effect on the cotton leaf worm Spodoptera littoralis. The third instar larvae fed for two days on treated leaves were more susceptible to plant extracts and to their ethyl acetate and chloroform fractions. The active lowest concentration (5%) of the flower fractions showed no significant effect on the percent reduction of emerged adult parasitoids, Microplitis rufiventris Kok. GC/MS analysis revealed that the major constituents in ethyl acetate fraction were 3-dihydro-methylene-2- (3H) furanone (17.8%), jasmolin I (15.6%), carveol 1 (13.6%), phosphoric acid, tributyl ester (11.4%) and cinerin II (11.1%), while those of chloroform fraction were 5-hydroxy-3 methyl-1H-pyrazole (42.7%) and carveol 1(24.8%). The medicinal plant C. coronarium seems to be a promising plant for application in integrated pest management due to its safety to the surrounding environment.

  12. Combination of 768-well microplate array diagonal gel electrophoresis with duplex PCR of X and Y chromosome markers for quality control of epidemiological DNA banks.

    Science.gov (United States)

    Huang, Shuwen; Chen, Xiao-he; Day, Ian N M

    2006-08-01

    Large DNA banks for human epidemiological studies have become an increasingly important research tool. The power of genotype-phenotype studies is dependent both on the quality of phenotyping and of genotyping and of correct linking of phenotypes to genotypes. Samples must be tracked through numerous steps between subject or patient and post-genotypic data. Only one phenotype, sex, has a perfect and binary correlation with genotype. In mixed sex studies, it may be advantageous for purposes of quality control to keep sexes mixed during the steps from acquisition to DNA bank, in order to be able to check later for sample swaps. We have designed a duplex PCR combining an amplicon from MAOA marking the X chromosome and an amplicon from DDX3Y marking the Y chromosome. We combined this with a simple economical palmtop sized 768-well microplate compatible electrophoresis system developed in-house for examination of duplex PCR products. We applied this quality control test in the validation of two DNA banks.

  13. Juan de Pitano y Bolívar, platero vitoriano del siglo XVI

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    Martín Vaquero, Rosa

    1997-03-01

    Full Text Available Juan de Pitano y Bolívar is one of the most important silversmiths of the second half of the 16th century in Vitoria. He was the successor to one of Vitoria's oldest silversmiths which had its origins in the previous century. Here we provide a biography of the man and present ten pieces of his work in which we are able to appreciated their quality together with the artistic techniques employed. All of the pieces bear his mark: PI/TANO. He was a master of his art and participated in all three of the styles which dominated the art of the silversmith in the 16th century: Gothic, Renaissance and Mannerism.No disponible

  14. San Juan National Forest Land Management Planning Support System (LMPSS) requirements definition

    Science.gov (United States)

    Werth, L. F. (Principal Investigator)

    1981-01-01

    The role of remote sensing data as it relates to a three-component land management planning system (geographic information, data base management, and planning model) can be understood only when user requirements are known. Personnel at the San Juan National Forest in southwestern Colorado were interviewed to determine data needs for managing and monitoring timber, rangelands, wildlife, fisheries, soils, water, geology and recreation facilities. While all the information required for land management planning cannot be obtained using remote sensing techniques, valuable information can be provided for the geographic information system. A wide range of sensors such as small and large format cameras, synthetic aperture radar, and LANDSAT data should be utilized. Because of the detail and accuracy required, high altitude color infrared photography should serve as the baseline data base and be supplemented and updated with data from the other sensors.

  15. Mago, coleccionista, aguafiestas: oficios de la poesía en Juan Manuel Roca

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    Patricia Trujillo Montón

    2012-01-01

    Full Text Available Este ensayo rastrea algunas metáforas del poeta y la poesía en la obra de Juan Manuel Roca de 1990 al día de hoy. Estas metáforas son el medio de una reflexión acerca de la función de la poesía, y de los problemas que enfrenta su escritura hoy día. Cada poema plantea una solución diferente, y el conjunto crea una constelación de posibilidades, a veces complementarias, a veces contrastantes, que pone de relieve el carácter precario y problemático, pero también necesario, de la poesía a finales del siglo veinte y comienzos del veintiuno.

  16. La Reforma Universitaria como Kulturkampf. La lectura gramsciana de Juan Carlos Portantiero

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    Natalia Bustelo

    2013-04-01

    Full Text Available En las últimas décadas hemos asistido a un renovado interés por la temprana recepción argentina de las categorías analíticas de Antonio Gramsci. Compartiendo ese interés, el presente trabajo se propone analizar las novedades introducidas por la lectura gramsciana de la Reforma Universitaria que propone Juan Carlos Portantiero en la década del setenta. Para ello, el trabajo comienza por ubicar las tesis de Estudiantes y política en América Latina. El proceso de la reforma universitaria (1918-1938 en el marco de las interpretaciones sobre la Reforma, para luego explicitar el proyecto político-intelectual en el que participa Portantiero en el momento de elaboración de su lectura, y concentrarse en las principales tesis del autor.

  17. Monitoreo de la calidad de datos GPS continuo: la estacion UNSJ (San Juan, Argentina

    Directory of Open Access Journals (Sweden)

    Alfredo Herrada

    2010-06-01

    Full Text Available Como parte de la red de referencia de operación continua de Argentina, la estación GPS (Global Positioning System denominada UNSJ (Universidad Nacional de San Juan fue establecida en la ciudad de San Juan el 6 de Marzo de 2007. Los datos registrados de UNSJ son ampliamente utilizados en aplicaciones catastrales, y sirven como base para la definición de los marcos de referencia geodésicos nacional y regional. Como una componente fundamental de la infraestructura geodésica, resulta conveniente un eficiente control de calidad de los datos crudos y el monitoreo de la estabilidad de una estación GPS de referencia. En este trabajo se presentan los resultados del control de calidad de las observaciones UNSJ luego de dos anos de operación. Para contro l ar y caracterizar el desempeno del receptor GPS y además el medio ambiente de la estación, se eligieron cuatro índices. Ellos son el número de observaciones, multicamino en L1, multicamino en L2 y ocurrencia de saltos de ciclos. También, se evaluó la estabilidad de largo término de la estación UNSJ a través del análisis de las series temporales de las coordenadas semanales provistas por los centros de cálculo SIRGAS (Sistema de Referencia Geocéntrico para las Américas. Completa este estudio el análisis de las coordenadas calculadas por distintos servicios de procesamiento disponibles en Internet. Nuestros resultados indican que durante el período analizado, el funcionamiento de la estación UNSJ fue satisfactorio, produciendo índices de calidad que son aceptables para estándares internacionales.As a part of the Argentine continuously operating reference station network, a GPS (Global Positioning System station named UNSJ (Universidad Nacional de San Juan was established in San Juan city on 6th March 2007. The recorded data of UNSJ are widely applied to cadastral surveys and serve as the basis for defining national and regional geodetic reference frames. As a key component of the

  18. Modern Monarchs and Democracy: Thailand’s Bhumibol Adulyadej and Juan Carlos of Spain

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    Serhat Ünaldi

    2012-01-01

    Full Text Available The history of democracy is typically a history of struggle against monarchs and other such autocrats. The elevation of one person over others by virtue of blood and birth has come to be seen as anachronistic; yet some monarchies have managed to survive to this day. This paper analyses two examples of the uneasy coalition between popular sovereignty and royal leadership that is constitutional monarchy. Whereas Juan Carlos of Spain has been described as having steered Spain away from dictatorship, Bhumibol of Thailand has come under scrutiny for allegedly lacking a principled approach to democracy. I argue that structural as much as personal factors influenced the ways in which the two monarchies were legitimised – one by positively responding to the modern aspirations of the king’s subjects, giving him a “forward legitimacy,” the other by revitalising the king’s traditional charisma and opting for “backward legitimacy.”

  19. DINÁMICA ESPACIO-TEMPORAL DEL HUMEDAL JUAN AMARILLO ENTRE 1950 - 2005

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    BEJARANO MORA PATRICIA ANDREA

    2009-04-01

    Full Text Available

    RESUMEN

    Se analizó la dinámica del Humedal Juan Amarillo durante el periodo comprendido entre los años 1950-2005. Los resultados muestran que la configuración paisajística del ecosistema ha cambiado notoriamente con una tendencia al incremento en el número, tamaño y tipo de parches. Igualmente se presentan diferencias estructurales importantes entre los diversos periodos de tiempo analizados, ocasionados principalmente por la disminución y desaparición de espejos de agua y el aumento de pasto kikuyo (Pennisetum clandestinum. En cuanto al arreglo espacial de los elementos paisajísticos, no sucedieron cambios importantes en los patrones de distribución espacial de los elementos paisajísticos del humedal. De acuerdo con la dimensión fractal, los elementos del paisaje presentan dos tendencias en cuanto a su complejidad: los elementos introducidos poseen las formas más complejas y los elementos propios del ecosistema tienen formas más regulares. Finalmente, la dinámica del humedal fue modelada con el fin de interpretar cómo las múltiples interacciones entre los elementos paisajísticos han influido en la configuración del humedal a lo largo del tiempo.

    Palabras clave: Humedal Juan Amarillo, estructura, composición, dimensión fractal.


    ABSTRACT

    The dynamics of Juan Amarillos’ wetland were analyzed during 55 year between 1950 and 2005. The results showed that the landscape configuration of the ecosystems has dramatically changed within a trend to increase in number, size, and patch type. Likewise, important structural differences came up between the various periods in question; such differences appear to be related to the reduction and disappearance of the pond and the significant increase of the non native kikuyo grass (Pennisetum clandestinum. Regarding the spatial

  20. Un acercamiento a la narración fotográfica de Juan Rulfo

    OpenAIRE

    2016-01-01

    El artículo trata de describir las diferentes facetas de la obra fotográfica de Juan Rulfo: su aspecto técnico, su lugar en el contexto de la fotografía mexicana y su aportación antropológica. El análisis se concentrará ante todo en la fotografía antropológica y arquitectónica para mostrar la visión de decadencia que predomina en la producción artística de Rulfo. Se tratará también el tema de la narración fotográfica, las fotografías sin foco como un punto de connotación entre el arte visual ...