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Sample records for interrogans mediante rcp

  1. Identificación de antígenos inmunorreactivos de Leptospira interrogans Identification of immunoreactive antigens of Leptospira interrogans

    Directory of Open Access Journals (Sweden)

    A. Carrizo

    2009-09-01

    Full Text Available Se estudió un lote de 28 sueros de llama (Lama gama de la provincia de Jujuy, Argentina, a fin de identificar antígenos inmunorreactivos contra Leptospira interrogans. Se utilizaron distintas preparaciones antigénicas de la bacteria para estudiar la inmunorreactividad mediante microaglutinación (MAT, ELISA y Western inmunoblot. Un pool de sueros bovinos positivos a la MAT fue empleado como control. Todos los sueros de llama fueron negativos mediante MAT e igual resultado se observó mediante ELISA. Dos de los 28 sueros de llama y el pool de sueros bovinos positivos, al ser evaluados por Western inmunoblot, arrojaron resultados positivos y permitieron identificar proteínas inmunorreactivas. Por MALDI-TOF se logró establecer que la proteína asociada a los dos sueros de llama inmunorreactivos era una flagelina periplásmica de Leptospira interrogans serovar Lai STR, mientras que la asociada al pool de sueros bovinos positivos a Leptospira sp. se trataba de una lipoproteína de la membrana externa de Leptospira interrogans serovar Ballum, LipL21. Estas proteínas podrían ser utilizadas en el diseño de un nuevo ELISA aplicado al diagnóstico temprano de leptospirosis, ya sea en distintos tipos de ganado como así también en reservorios silvestres.A batch of 28 llama (Lama gama sera from Jujuy province in Argentina was studied in order to identify immune reactive antigens to Leptospira interrogans. Different antigenic preparations from the bacterium were used to study the immune reactivity by the microagglutinattion (MAT, ELISA and Western immunoblot tests. A control pool of positive bovine sera was used. All the llama sera were negative to MAT as well as to ELISA. Two of the llama sera and the positive bovine sera pool rendered positive results when evaluated by Western immunoblot, allowing the identification of immune reactive proteins. These proteins were identified by MALDI-TOF. A periplasmic flagellin of Leptospira interrogans serovar

  2. NEW WILDLIFE HOSTS OF Leptospira interrogans IN CAMPECHE, MEXICO

    OpenAIRE

    ESPINOSA-MARTÍNEZ,Deborah V.; SÁNCHEZ-MONTES,Daniel Sokani; León-Paniagua,Livia; Ríos-Muñoz, César A.; BERZUNZA-CRUZ,Miriam; BECKER,Ingeborg

    2015-01-01

    Leptospira interrogans has been identified to cause leptospirosis, a widespread zoonotic disease that has been identified in domestic and wild animals. This work analyzed kidneys from two species of wild rodents from the state of Campeche, Mexico. Analyses were made by PCR using specific primers for detection of Leptospira interrogans DNA. The rodent species that tested positive were Heteromys gaumeri and Ototylomys phyllotis, both of which are new hosts for the bacteria in Southeastern Mexic...

  3. RCP 劲性复合桩的应用%The application of RCP composite pile

    Institute of Scientific and Technical Information of China (English)

    宋阳

    2016-01-01

    介绍了 RCP 劲性复合桩的技术原理,结合工程案例,从定点放线、搅拌钻进、水泥浆制作、桩芯植入等方面,阐述了 RCP 劲性复合桩的施工工艺流程及要点,并介绍了该技术的优点,可为其他项目桩基工程提供参考。%This paper introduced the technology principle of RCP composite piles,combining with the engineering case,starting from aspects of fixed-point sampling,mixing drilling,cement slurry prefabrication and inside pile planting,describes the construction process and points of RCP composite pile,and introduces the advantages of the technology,can provide a reference for other projects pile works.

  4. Biochemical characterization of riboflavin carrier protein (RCP) in prostate cancer.

    Science.gov (United States)

    Johnson, Tanya; Ouhtit, Allal; Gaur, Rajiv; Fernando, Augusta; Schwarzenberger, Paul; Su, Joseph; Ismail, Mohamed F; El-Sayyad, Hassan I; Karande, Anjali; Elmageed, Zakaria Abd; Rao, Prakash; Raj, Madhwa

    2009-01-01

    Riboflavin carrier protein (RCP) is a growth- and development-specific protein. Here, we characterized the expression of this protein in prostate cancer by polyclonal and monoclonal antibodies against chicken RCP. RCP was localized to both androgen-dependent and independent prostate cancer cell lines. Compared to controls, RCP was over-expressed in all 45 prostate adenocarcinomas, irrespective of the Gleason's score or the stage of the disease. The identified RCP had a molecular weight of 38 kDa, similar to RCP purified from chicken. Presence of this protein was also confirmed by siRNA inhibition analysis. Antibodies to chicken RCP inhibited incorporation of tritiated thymidine into DNA and prevented riboflavin uptake in PC3 prostate cancer cells, suggesting a critical function of this protein in prostate cancer cell growth. These data suggest that RCP can be used as a tumor biomarker in prostate cancer.

  5. Medio EMJH modificado para el cultivo de Leptospira interrogans serogrupo Ballum Modified EMJH medium for cultivation of Leptospira interrogans serogroup Ballum

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    A. González

    2006-04-01

    Full Text Available El serogrupo Ballum agrupa cepas de crecimiento fastidioso, con requerimientos nutricionales más exigentes que otras cepas patógenas de Leptospira. Fue evaluada la influencia de 37 compuestos nutricionales sobre el crecimiento de Leptospira interrogans serogrupo Ballum, tomando como base para el estudio al medio sintético EMJH. El crecimiento microbiano fue estimado espectrofotométricamente y por conteo directo en cámara de Petroff-Hausser. La estabilidad de la virulencia fue evaluada en hamsters mediante el cálculo de la dosis letal media. La estabilidad de la antigenicidad fue evaluada mediante Western blotting con antisuero policlonal específico. Bajo condiciones de cultivo controladas se logró triplicar los rendimientos de biomasa comúnmente obtenidos en el medio EMJH sin afectación de la virulencia y antigenicidad tras el incremento de la concentración de Tween 80 y la incorporación de acetato de sodio y extracto de carne. El incremento de la concentración de al menos 6 componentes del EMJH o la incorporación de una variedad de nuevos nutrientes no estimularon apreciablemente los rendimientos de biomasa o la velocidad específica de crecimiento del microorganismo. Los resultados obtenidos permiten disponer de un medio de cultivo enriquecido capaz de sustentar elevados rendimientos de biomasa de este serogrupo exigente de mayor circulación en humanos en Cuba.Strains within the Ballum serogroup of spirochete Leptospira show fastidious growth with more exigent nutritional requirements than those of other Leptospira pathogenic strains. The influence of 37 nutritional compounds on the growth of Leptospira interrogans serogroup Ballum was investigated employing the synthetic EMJH medium as the base for the study. Microbial growth was estimated spectrophotometrically and direct counts were performed with a Petroff-Hausser counting chamber. Virulence stability was evaluated by calculating the mean lethal dose in hamsters

  6. Genome features of Leptospira interrogans serovar Copenhageni

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    A.L.T.O. Nascimento

    2004-04-01

    Full Text Available We report novel features of the genome sequence of Leptospira interrogans serovar Copenhageni, a highly invasive spirochete. Leptospira species colonize a significant proportion of rodent populations worldwide and produce life-threatening infections in mammals. Genomic sequence analysis reveals the presence of a competent transport system with 13 families of genes encoding for major transporters including a three-member component efflux system compatible with the long-term survival of this organism. The leptospiral genome contains a broad array of genes encoding regulatory system, signal transduction and methyl-accepting chemotaxis proteins, reflecting the organism's ability to respond to diverse environmental stimuli. The identification of a complete set of genes encoding the enzymes for the cobalamin biosynthetic pathway and the novel coding genes related to lipopolysaccharide biosynthesis should bring new light to the study of Leptospira physiology. Genes related to toxins, lipoproteins and several surface-exposed proteins may facilitate a better understanding of the Leptospira pathogenesis and may serve as potential candidates for vaccine.

  7. Leptospira interrogans serotype hardjo in dairy cows

    Directory of Open Access Journals (Sweden)

    Vidić Branka M.

    2003-01-01

    Full Text Available Data on L. hardjo infection of dairy cows in the world pint out its important role in the occurrence of health and economic problem. L. interrogans serotype hardjo has been described as the cause of miscarriages, stillbirts, or the birhs of poorly vital calves, agalactia, mastitis, and low fertility in cows. Two L. hardjo genotypes have been identified in cows, namely, hardjopraitno and hardjobovis. Serological investigations have established a drastic increase in this leptospiral infection in cows. L. hardjo has become adapted to cattle as the primary host, so that an infection is maintained in herds and becomes deeply rooted because of the permanent presence of the source of infection. It was believed that sheep were accidental hosts, but the latest research suggest that they are yet another, transitory, host for maintining this leptospira serotype. L. hardjo is also important from the aspect of human health, especially of persons who are professionally exposed to this infection. L. hardjo infection is detected using serological tests and by proving the presence of leptospira. The medicine of choice in the therapy of leptospiral infections is streptomycin (DSM. Therapy using oxytetracyclines for clinical mastitis was also proven effective. Treatment is most successful in the early stage of the disease. A single dose of streptomycin administered in infected herds reduces the duration period of leptospira excretion through urine, thus preventing the spread of infection thorugh contaminated urine. The basic components of the plan to contain leptospira are the following: serological investigations, sanitary-higiene measures, the elimination of animals which excrete leptospira through urine, therapy, vaccination, quarantine.

  8. Protección inducida por nanococleatos derivados de proteoliposomas de Leptospira interrogans serovar Canicola

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    Beatriz Tamargo

    2012-04-01

    Full Text Available Desde los años 20 del pasado siglo, hasta el presente, en el mundo se han desarrollado y empleado vacunas de células enteras contra la leptospirosis que confieren una corta inmunidad; la mayoría no adyuvadas y dirigidas, fundamentalmente, contra los diferentes serogrupos de la especie Leptospira interrogans, contenidos en las preparaciones. Numerosos han sido los intentos realizados para lograr una formulación vacunal más pura, efectiva, de amplio espectro y duración de la protección que las bacterinas de células enteras inactivadas. Sin embargo, hasta el momento no se ha registrado ninguna vacuna con tales características. En el presente trabajo se obtuvieron antígenos de membrana externa a partir de una cepa cubana autóctona (Cepa 87, L. interrogans serovar Canicola, mediante una modificación de la tecnología para la producción de vesículas de membrana, patentada por investigadores del Instituto Finlay. Estos antígenos con estructura nanoproteoliposómica fueron formulados/adyuvados mediante diferentes estrategias, logrando cinco preparaciones con estructura coclear, que constituyen nanopartículas de aproximadamente 100 a 150 nm de largo y entre 15 a 30 nm de diámetro. Los inmunógenos se inocularon en el biomodelo Mesocrisetus aureatus, con dos dosis e intervalo de seis semanas. El reto fue realizado con 100.000 DL 50 . Los resultados demuestran que las nuevas formulaciones vacunales confieren protección frente al reto homólogo y fueron capaces de eliminar el estado de portador, lo que unido a la robustez del método de preparación, el mayor nivel de pureza, en comparación con las bacterinas, y la no necesidad del hidróxido de aluminio, las convierten en una alternativa de interés para continuar su desarrollo.

  9. The concept of rural community practice (RCP).

    Science.gov (United States)

    Nielsen, N Ole; Evans, Brian; King, Lonnie J

    2006-01-01

    The need to devote more human resources to veterinary public practice to cope with escalating threats to biological security, public health, and economic prosperity, while also addressing societal value changes, has been widely recognized and supported. Most envisage increasing the numbers of veterinarians in government employment. Why not at least combine this initiative, wherever possible, with far greater involvement of rural practitioners to deliver contractual public-practice services and provide an enhanced community interface? This could make the difference between having a local practice in a community or none at all, as well as promising to be more cost effective. The concept of rural community practice (RCP) envisages combining traditional services provided in a "mixed-animal" veterinary practice with an expanded portfolio of public-practice and communication services that meet the emerging animal, public, and ecosystem health needs of the collective community, not just those of animal owners. These services could include those involving active sentinel surveillance programs for both domestic animal and wildlife diseases; on-farm food safety; bio-security; traceability and export certification and audit programs; disease investigation, including foreign animal diseases; surge capacity emergency response; managing for ecosystem health; and client and community education. An expanded practice team of animal-health professionals and technologists, led by veterinarians, would deliver these services. This RCP approach should have the potential to make rural practice more attractive from economic, lifestyle, and job-satisfaction perspectives; to enhance the visibility and recognition of the profession; and to respond to changing and new societal needs. It also promises to maintain a stable network of veterinary practices in rural communities. In addition, the recognition of veterinary medicine as a public good should provide for consideration of increased

  10. New wildlife hosts of Leptospira interrogans in Campeche, Mexico.

    Science.gov (United States)

    Espinosa-Martínez, Deborah V; Sánchez-Montes, Daniel Sokani; León-Paniagua, Livia; Ríos-Muñoz, César A; Berzunza-Cruz, Miriam; Becker, Ingeborg

    2015-01-01

    Leptospira interrogans has been identified to cause leptospirosis, a widespread zoonotic disease that has been identified in domestic and wild animals. This work analyzed kidneys from two species of wild rodents from the state of Campeche, Mexico. Analyses were made by PCR using specific primers for detection of Leptospira interrogans DNA. The rodent species that tested positive were Heteromys gaumeri and Ototylomys phyllotis, both of which are new hosts for the bacteria in Southeastern Mexico. These records provide new insights into the disease's transmission that should be studied carefully in order to identify other potential host species, including humans, which are at risk of becoming infected if they are in contact with infected wildlife.

  11. NEW WILDLIFE HOSTS OF Leptospira interrogans IN CAMPECHE, MEXICO

    Directory of Open Access Journals (Sweden)

    Deborah V. ESPINOSA-MARTÍNEZ

    2015-04-01

    Full Text Available Leptospira interrogans has been identified to cause leptospirosis, a widespread zoonotic disease that has been identified in domestic and wild animals. This work analyzed kidneys from two species of wild rodents from the state of Campeche, Mexico. Analyses were made by PCR using specific primers for detection of Leptospira interrogans DNA. The rodent species that tested positive were Heteromys gaumeri and Ototylomys phyllotis, both of which are new hosts for the bacteria in Southeastern Mexico. These records provide new insights into the disease’s transmission that should be studied carefully in order to identify other potential host species, including humans, which are at risk of becoming infected if they are in contact with infected wildlife.

  12. Caracterization of the SOS response in Leptospira interrogans sorovar Copenhageni

    OpenAIRE

    Luciane Schons da Fonseca

    2015-01-01

    Leptospira is a basal genus in an ancient group of bacteria, the spirochetes. The pathogenic species are responsible for leptospirosis, a disease with worldwide distribution and of public health importance in developed tropical countries. L. interrogans serovar Copenhageni is the agent for the majority of human leptospirosis in Brazil. In this work, we used a great variety of experimental approaches to characterize the SOS system in this serovar, to identify its impact in general DNA damage r...

  13. Multilocus Sequence Analysis for Typing Leptospira interrogans and Leptospira kirschneri▿ †

    OpenAIRE

    2009-01-01

    Fifty-three strains belonging to the pathogenic species Leptospira interrogans and Leptospira kirschneri were analyzed by multilocus sequence analysis. The species formed two distinct branches. In the L. interrogans branch, the phylogenetic tree clustered the strains into three subgroups. Genogroups and serogroups were superimposed but not strictly.

  14. The RCP-Rab11 complex regulates endocytic protein sorting.

    Science.gov (United States)

    Peden, Andrew A; Schonteich, Eric; Chun, John; Junutula, Jagath R; Scheller, Richard H; Prekeris, Rytis

    2004-08-01

    Rab 11 GTPase is an important regulator of endocytic membrane traffic. Recently, we and others have identified a novel family of Rab11 binding proteins, known as Rab11-family interacting proteins (FIPs). One of the family members, Rab coupling protein (RCP), was identified as a protein binding to both Rab4 and Rab11 GTPases. RCP was therefore suggested to serve a dual function as Rab4 and Rab11 binding protein. In this study, we characterized the cellular functions of RCP and mapped its interactions with Rab4 and Rab11. Our data show that RCP interacts only weakly with Rab4 in vitro and does not play the role of coupling Rab11 and Rab4 in vivo. Furthermore, our data indicate that the RCP-Rab11 complex regulates the sorting of transferrin receptors from the degradative to the recycling pathway. We therefore propose that RCP functions primarily as a Rab11 binding protein that regulates protein sorting in tubular endosomes.

  15. Synthesis, properties and formation of (RCp)Co- and (RCp)Rh-stabilized[4.8]3cyclacene derivatives.

    Science.gov (United States)

    Kornmayer, Stefan C; Hellbach, Björn; Rominger, Frank; Gleiter, Rolf

    2009-01-01

    Metal-stabilized belts: A torus, 3, consisting of three four- and three eight-membered conjugated rings and stabilized by (RCp)Co- and (RCp)Rh- units, was generated by irradiation of [(RCp)Co(CO)(2)] and [(RCp)Rh(C(2)H(4))(2)], respectively, and 1.Eleven metal stabilized [4.8](3)cyclacene derivatives were synthesized. The substances were prepared in one-pot reactions by irradiation of a solution of 5,6,11,12-tetradehydro-dibenzo[a,e]cyclooctatetraene and the corresponding cobalt reagents or rhodium compounds. The resulting cyclacene derivatives reveal D(3h) symmetry in solution. In the solid state the hoop shaped systems crystallize in layers, which are intercalated with solvent layers. To unravel the mechanism of the one-pot reaction we isolated an intermediate, which shows almost planar cyclooctatetraene rings.

  16. 互联网络RCP(n)的路由算法%Routing algorithms on interconnection networks RCP(n)

    Institute of Scientific and Technical Information of China (English)

    刘宏英; 高太平

    2008-01-01

    在对互联网络RCP(Ringed Crossed cube Petersen)拓扑结构研究的基础上,利用RCP(n)网络具有正则性,良好的可扩展性,以及具有比Qn、HP(n)、RHP(n)网络更短的直径和更小的构造开销这些特性,给出了RCP(n)的单播路由算法和广播路由算法,并证明了RCP(n)比Qn、HP(n)、RHP(n)网络具有更好的路由性能.

  17. Von Roll RCP method - first experiences; Von Roll RCP - Verfahren. Erste Erfahrungen

    Energy Technology Data Exchange (ETDEWEB)

    Capitaine, P.; Engweiler, J. [Roll Umwelttechnik AG, Zuerich (Switzerland)

    1998-09-01

    The RCP method was designed as a residue-optimised alternative to the thermally optimised grate firing of residual wastes. Its technical realisation and development to market maturity took no more than 5 years. In the first process stage the waste is converted to high-carbon pyrolysis charcoal and high-rank gas in the absence of oxygen. In the second stage these substances are oxidised by addition of oxygen. The resulting temperature causes the non-combustible constituents of the slag to melt. In a third, optional, stage this molten slag can be liberated of (heavy) metals to such an extent that it can subsequently be used directly as additive for grinding. Further exhaust gas treatment is facilitated by the use of a circulatory fluidised-bed secondary combustion chamber. Despite the reduced flue gas volume and resultant higher pollutant concentrations in the crude gas, overall emissions are lower than in conventional plants. [Deutsch] Das reststoffoptimierte RCP Verfahren ist als Alternative zur thermisch optimierten Rostverbrennung von Restabfaellen konzipiert. In nur fuenf Jahren wurde das Verfahren technisch umgesetzt und zur Marktreife entwickelt. In einer ersten Stufe des Verfahrens wird der Abfall unter Luftabschluss in einen kohlenstoffreichen Pyrolysekoks und eine heizwertreiches Gas umgesetzt. Im zweiten Schritt werden diese Stoffe unter Zugabe von Sauerstoff oxidiert. Dabei treten Temperaturen auf, bei denen die nichtbrennbaren Bestandteile der Schlacke schmelzen. Optional wird diese Schmelzschlacke in einem dritten Schritt derart von (Schwer-) Metallen befreit, dass sie anschliessend direkt als Zement-Zumahlstoff eingesetzt werden kann. Die Nutzung der zirkulierenden Wirbelschicht - Nachbrennkammer zur Abgasbehandlung vereinfacht die weitergehende Abgasbehandlung. Trotz verringertem Rauchgasvolumen und damit hoeheren Schadstoffkonzentrationen im Rohgas werden die Gesamtemissionen gegenueber konventionellen Anlagen verringert. (orig./SR)

  18. Crystallization and preliminary X-ray diffraction studies of ferredoxin reductase from Leptospira interrogans

    Energy Technology Data Exchange (ETDEWEB)

    Nascimento, Alessandro S.; Ferrarezi, Thiago [Instituto de Física de São Carlos, Universidade de São Paulo, Av. Trabalhador Saocarlense 400, São Carlos, SP, 13560-970 (Brazil); Catalano-Dupuy, Daniela L.; Ceccarelli, Eduardo A. [Facultad de Ciencias Bioquímicas y Farmacéuticas, Molecular Biology Division, Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario (Argentina); Polikarpov, Igor, E-mail: ipolikarpov@if.sc.usp.br [Instituto de Física de São Carlos, Universidade de São Paulo, Av. Trabalhador Saocarlense 400, São Carlos, SP, 13560-970 (Brazil)

    2006-07-01

    Crystals adequate for X-ray diffraction analysis have been prepared from L. interrogans ferredoxin-NADP{sup +} reductase. Ferredoxin-NADP{sup +} reductase (FNR) is an FAD-containing enzyme that catalyzes electron transfer between NADP(H) and ferredoxin. Here, results are reported of the recombinant expression, purification and crystallization of FNR from Leptospira interrogans, a parasitic bacterium of animals and humans. The L. interrogans FNR crystals belong to a primitive monoclinic space group and diffract to 2.4 Å resolution at a synchrotron source.

  19. Role of CGRP-Receptor Component Protein (RCP) in CLR/RAMP Function

    OpenAIRE

    Dickerson, Ian M.

    2013-01-01

    The receptor for calcitonin gene-related peptide (CGRP) and adrenomedullin (AM) requires an intracellular peripheral membrane protein named CGRP-receptor component protein (RCP) for signaling. RCP is required for CGRP and AM receptor signaling, and it has recently been discovered that RCP enables signaling by binding directly to the receptor. RCP is present in most immortalized cell lines, but in vivo RCP expression is limited to specific subsets of cells, usually co-localizing with CGRP-cont...

  20. Leptospira interrogans induces uterine inflammatory responses and abnormal expression of extracellular matrix proteins in dogs.

    Science.gov (United States)

    Wang, Wei; Gao, Xuejiao; Guo, Mengyao; Zhang, Wenlong; Song, Xiaojing; Wang, Tiancheng; Zhang, Zecai; Jiang, Haichao; Cao, Yongguo; Zhang, Naisheng

    2014-10-01

    Leptospira interrogans (L. interrogans), a worldwide zoonosis, infect humans and animals. In dogs, four syndromes caused by leptospirosis have been identified: icteric, hemorrhagic, uremic (Stuttgart disease) and reproductive (abortion and premature or weak pups), and also it caused inflammation. Extracellular matrix (ECM) is a complex mixture of matrix molecules that is crucial to the reproduction. Both inflammatory response and ECM are closed relative to reproductive. The aim of this study was to clarify how L. interrogans affected the uterus of dogs, by focusing on the inflammatory responses, and ECM expression in dogs uterine tissue infected by L. interrogans. In the present study, 27 dogs were divided into 3 groups, intrauterine infusion with L. interrogans, to make uterine infection, sterile EMJH, and normal saline as a control, respectively. The uteruses were removed by surgical operation in 10, 20, and 30 days, respectively. The methods of histopathological analysis, ELISA, Western blot and qPCR were used. The results showed that L. interrogans induced significantly inflammatory responses, which were characterized by inflammatory cellular infiltration and high expression levels of tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) in uterine tissue of these dogs. Furthermore, L. interrogans strongly down-regulated the expression of ECM (collagens (CL) IV, fibronectins (FN) and laminins (LN)) in mRNA and protein levels. These data indicated that strongly inflammatory responses, and abnormal regulation of ECM might contribute to the proliferation of dogs infected by L. interrogans.

  1. An Experience of Thermowell Design in RCP Test Facility

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Y. S.; Kim, B. D.; Youn, Y. J.; Jeon, W. J.; Kim, S.; Bae, B. U.; Cho, Y. J.; Choi, H. S.; Park, J. K; Cho, S. [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2015-10-15

    Flow rates for the test should vary in the range of 90% to 130% of rated flowrate under prototypic operational conditions, as shown in Table 1. Generally for the flow control, a combination of a control valve and an orifice was used in previous RCP test facilities. From the commissioning startup of the RCP test facility, it was found the combination of valve and orifice induced quite a large vibration for the RCP. As a solution to minimize the vibration and to facilitate the flowrate control, one of KAERI's staff suggested a variable restriction orifice (VRO), which controls most of the required flowrates except highest flowrates, as shown in Fig. 2. For the highest flowrates, e.g., around run-out flowrate (130%), control valves in bypass lines were also used to achieve required flowrates. From a performance test, it was found the VRO is very effective measures to control flowrates in the RCP test facility. During the commissioning startup operation, one of thermowells located at the upstream of the RCP was cracked due to high speed coolant velocity, which was - fortunately - found under a leakage test before running the RCP test loop. The cracked thermowell, whose tapered-shank was detached from the weld collar after uninstalling, is shown in Fig. 3. As can be seen the figure, most of the cross-section at the root of the thermowell shank was cracked. In this paper, an investigation of the integrity of thermowells in the RCP test facility was performed according to the current code and overall aspects on the thermowell designs were also discussed. An RCP test facility has been constructed in KAERI. During the commissioning startup operation, one of thermowells was cracked due to high speed coolant velocity. To complete the startup operation, a modified design of thermowells was proposed and all the original thermowells were replaced by the modified ones. From evaluation of the original and modified designs of thermowells according to the recent PTC code, the

  2. RCP 钢筋混凝土管检测技术%On testing technique of RCP reinforced concrete pipes

    Institute of Scientific and Technical Information of China (English)

    焦利敏

    2016-01-01

    The paper introduces the testing programs of RCP reinforced concrete pipes,illustrates its testing points from the outline quality,geo-metric dimensioning,compressive strength,and internal water pressure,and points out the test evaluation for the reinforced concrete pipes,so as to be meaningful for the improvement of the drainage pipe quality.%介绍了 RCP 钢筋混凝土管的检测项目,从外观质量、几何尺寸、抗压强度、内水压力等方面,阐述了 RCP 钢筋混凝土管的检测要点,指出钢筋混凝土管的检测评定,对于提高排水管的质量有重要意义。

  3. The Role of Cgrp-Receptor Component Protein (Rcp) in Cgrp-Mediated Signal Transduction

    OpenAIRE

    Prado, M.A.; B. Evans-Bain; Santi, S. L.; Dickerson, I M

    2001-01-01

    The calcitonin gene-related peptide (CGRP)-receptor component protein (RCP) is a 17-kDa intracellular peripheral membrane protein required for signal transduction at CGRP receptors. To determine the role of RCP in CGRP-mediated signal transduction, RCP was depleted from NIH3T3 cells using antisense strategy. Loss of RCP protein correlated with loss of cAMP production by CGRP in the antisense cells. In contrast, loss of RCP had no effect on CGRP-mediated binding; therefore RCP is not acting as...

  4. OPR1000 RCP Flow Coastdown Analysis using SPACE Code

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Dong-Hyuk; Kim, Seyun [KHNP CRI, Daejeon (Korea, Republic of)

    2016-10-15

    The Korean nuclear industry developed a thermal-hydraulic analysis code for the safety analysis of PWRs, named SPACE(Safety and Performance Analysis Code for Nuclear Power Plant). Current loss of flow transient analysis of OPR1000 uses COAST code to calculate transient RCS(Reactor Coolant System) flow. The COAST code calculates RCS loop flow using pump performance curves and RCP(Reactor Coolant Pump) inertia. In this paper, SPACE code is used to reproduce RCS flowrates calculated by COAST code. The loss of flow transient is transient initiated by reduction of forced reactor coolant circulation. Typical loss of flow transients are complete loss of flow(CLOF) and locked rotor(LR). OPR1000 RCP flow coastdown analysis was performed using SPACE using simplified nodalization. Complete loss of flow(4 RCP trip) was analyzed. The results show good agreement with those from COAST code, which is CE code for calculating RCS flow during loss of flow transients. Through this study, we confirmed that SPACE code can be used instead of COAST code for RCP flow coastdown analysis.

  5. Future ozone and oxidants change under the RCP scenarios

    Science.gov (United States)

    Kim, Minjoong J.; Park, Rokjin J.; Ho, Chang-Hoi; Woo, Jung-Hun; Choi, Ki-Chul; Song, Chang-Keun; Lee, Jae-Bum

    2015-01-01

    We investigate ozone air quality changes in 2050 caused by global changes in climate and anthropogenic emissions of ozone precursors by using a global chemical transport model driven by meteorological fields from a general circulation model. We use projected emissions based on the Representative Concentration Pathway (RCP) scenarios and conduct model simulations to quantify the effects of climate and emission changes on future air quality, focusing on ozone in surface air. Our model results show that annual mean concentrations of surface ozone will be lower in 2050 relative to 2000 by -3.3, -3.7, and -4.2 ppbv under RCP6.0, RCP4.5, and RCP2.6, respectively. In contrast, the RCP8.5 projection results in a slight increase of 2.1 ppbv caused by a methane increase. The ozone reductions are driven primarily by decreases in NOx emission, which dominate the climate penalty on ozone driven by temperature increases. We also estimate the effect of 21st century climate change on ozone air quality, assuming no changes in anthropogenic emissions of ozone precursors in the future. We further use a statistical method to analyze the results in order to quantify the effect of each meteorological variable change on ozone concentration in summer. Temperature increase is found to result in ozone increases of up to 2.2 ppbv over land. Ozone over the oceans, however, is largely reduced with specific humidity increase, particularly in the Northern Hemisphere, where the ozone concentration decreases by 0.8 ppbv. We find that future increases in natural NOx emissions from lightning and soil make an important contribution to the formation of nitric acid and might seriously offset future decreases in nitrogen deposition caused by anthropogenic NOx emission reduction.

  6. The Role of Cgrp-Receptor Component Protein (Rcp in Cgrp-Mediated Signal Transduction

    Directory of Open Access Journals (Sweden)

    M. A. Prado

    2001-01-01

    Full Text Available The calcitonin gene-related peptide (CGRP-receptor component protein (RCP is a 17-kDa intracellular peripheral membrane protein required for signal transduction at CGRP receptors. To determine the role of RCP in CGRP-mediated signal transduction, RCP was depleted from NIH3T3 cells using antisense strategy. Loss of RCP protein correlated with loss of cAMP production by CGRP in the antisense cells. In contrast, loss of RCP had no effect on CGRP-mediated binding; therefore RCP is not acting as a chaperone for the CGRP receptor. Instead, RCP is a novel signal transduction molecule that couples the CGRP receptor to the cellular signal transduction machinery. RCP thus represents a prototype for a new class of signal transduction proteins that are required for regulation of G protein-coupled receptors.

  7. Ecology of Leptospira interrogans in Norway rats (Rattus norvegicus in an inner-city neighborhood of Vancouver, Canada.

    Directory of Open Access Journals (Sweden)

    Chelsea G Himsworth

    Full Text Available BACKGROUND: Leptospira interrogans is a bacterial zoonosis with a worldwide distribution for which rats (Rattus spp. are the primary reservoir in urban settings. In order to assess, monitor, and mitigate the risk to humans, it is important to understand the ecology of this pathogen in rats. The objective of this study was to characterize the ecology of L. interrogans in Norway rats (Rattus norvegicus in an impoverished inner-city neighborhood of Vancouver, Canada. METHODOLOGY/PRINCIPAL FINDINGS: Trapping was performed in 43 city blocks, and one location within the adjacent port, over a 12 month period. Kidney samples were tested for the presence of L. interrogans using PCR and sequencing. A multivariable model was built to predict L. interrogans infection status in individual rats using season and morphometric data (e.g., weight, sex, maturity, condition, etc. as independent variables. Spatial analysis was undertaken to identify clusters of high and low L. interrogans prevalence. The prevalence of L. interrogans varied remarkably among blocks (0-66.7%, and spatial clusters of both high and low L. interrogans prevalence were identified. In the final cluster-controlled model, characteristics associated with L. interrogans-infection in rats included weight (OR = 1.14, 95% CI = 1.07-1.20, increased internal fat (OR = 2.12, 95% CI = 1.06-4.25, and number of bite wounds (OR = 1.20, 95% CI = 0.96-1.49. CONCLUSIONS/SIGNIFICANCE: Because L. interrogans prevalence varied with weight, body fat, and bite wounds, this study suggests that social structure and interactions among rats may influence transmission. The prevalence and distribution of L. interrogans in rats was also highly variable even over a short geographic distance. These factors should be considered in future risk management efforts.

  8. Pathogenomic inference of virulence-associated genes in Leptospira interrogans.

    Directory of Open Access Journals (Sweden)

    Jason S Lehmann

    Full Text Available Leptospirosis is a globally important, neglected zoonotic infection caused by spirochetes of the genus Leptospira. Since genetic transformation remains technically limited for pathogenic Leptospira, a systems biology pathogenomic approach was used to infer leptospiral virulence genes by whole genome comparison of culture-attenuated Leptospira interrogans serovar Lai with its virulent, isogenic parent. Among the 11 pathogen-specific protein-coding genes in which non-synonymous mutations were found, a putative soluble adenylate cyclase with host cell cAMP-elevating activity, and two members of a previously unstudied ∼15 member paralogous gene family of unknown function were identified. This gene family was also uniquely found in the alpha-proteobacteria Bartonella bacilliformis and Bartonella australis that are geographically restricted to the Andes and Australia, respectively. How the pathogenic Leptospira and these two Bartonella species came to share this expanded gene family remains an evolutionary mystery. In vivo expression analyses demonstrated up-regulation of 10/11 Leptospira genes identified in the attenuation screen, and profound in vivo, tissue-specific up-regulation by members of the paralogous gene family, suggesting a direct role in virulence and host-pathogen interactions. The pathogenomic experimental design here is generalizable as a functional systems biology approach to studying bacterial pathogenesis and virulence and should encourage similar experimental studies of other pathogens.

  9. An Dynamic Realization for Eclipse RCP WizrdPage%Eclipse RCP WizrdPage动态实现方法

    Institute of Scientific and Technical Information of China (English)

    王舜燕; 康磊; 黄莎莉

    2007-01-01

    针对Eclipse RCP WizardPage的静态实现难以解决各输入页间数据关联的功能局限性问题,分析WizardPage的运行机理和动态实现的技术难点,提出一种基于观察者模式的Eclipse RCP WizardPage动态实现方法,使业务对象低耦合,逻辑清晰,为代码维护以及业务扩展奠定良好的基础,最后给出有代表性的需求模型及其实现编码.

  10. Heterogenic colonization patterns by Leptospira interrogans in Rattus norvegicus from urban slums

    Science.gov (United States)

    Santos, Ana Amélia Nunes; Figueira, Cláudio Pereira; dos Reis, Mitermayer Galvão; Costa, Federico; Ristow, Paula

    2015-01-01

    Abstract We evaluated the renal colonization by Leptospira interrogans in Rattus norvegicus (rats), as it is the major natural reservoir of urban leptospirosis. We caught 72 R. norvegicus, out of which 32 were found to be positive for L. interrogans by immunofluorescence assay. From these rats, we selected 17 and divided them into six groups based on the mass-age/sex. We performed the immunohistochemistry test against L. interrogans in the kidney sections of the rats and systematically counted the colonized tubules (CTs) in 20 fields. The proportion of positive fields varied from 5% to 95%. The number of CTs in 20 fields varied from 0.5 to 85.5. These differences were not related to age or sex of the animals. The characterization of leptospiral colonization patterns in the natural reservoirs is important to better understand the host-pathogen interactions in leptospirosis. PMID:26691476

  11. Prediction and systematic study of protein-protein interaction networks of Leptospira interrogans

    Institute of Scientific and Technical Information of China (English)

    SUN Jingchun; XU Jinlin; CAO Jianping; LIU Qi; GUO Xiaokui; SHI Tieliu; LI Yixue

    2006-01-01

    Leptospira interrogans serovar Lai is a pathogenic bacterium that causes a spirochetal zoonosis in humans and some animals. With its complete genome sequence available, it is possible to analyze protein-protein interactions from a whole- genome standpoint. Here we combine four recently developed computational approaches (gene fusion method, gene neighbor method, phylogenetic profiles method, and operon method) to predict protein-pro- tein interaction networks of Leptospira interrogans strain Lai. Through comprehensive analysis on in- teractions among proteins of motility and chemotaxis system, signal transduction, lipopolysaccaride bio- synthesis and a series of proteins related to adhesion and invasion, we provided information for further studying on its pathogenic mechanism. In addition, we also assigned 203 previously uncharacterized proteins with possible functions based on the known functions of its interacting partners. This work is helpful for further investigating L. interrogans strain Lai.

  12. Role of CGRP-receptor component protein (RCP) in CLR/RAMP function.

    Science.gov (United States)

    Dickerson, Ian M

    2013-08-01

    The receptor for calcitonin gene-related peptide (CGRP) and adrenomedullin (AM) requires an intracellular peripheral membrane protein named CGRP-receptor component protein (RCP) for signaling. RCP is required for CGRP and AM receptor signaling, and it has recently been discovered that RCP enables signaling by binding directly to the receptor. RCP is present in most immortalized cell lines, but in vivo RCP expression is limited to specific subsets of cells, usually co-localizing with CGRP-containing neurons. RCP protein expression correlates with CGRP efficacy in vivo, suggesting that RCP regulates CGRP signaling in vivo as it does in cell culture. RCP is usually identified in cytoplasm or membranes of cells, but recently has been observed in nucleus of neurons, suggesting an additional transcriptional role for RCP in cell function. Together, these data support an essential role for RCP in CGRP and AM receptor function, in which RCP expression enhances signaling of the CGRP or AM receptor, and therefore increases the efficacy of CGRP and AM in vivo.

  13. Genetic diversity among major endemic strains of Leptospira interrogans in China

    Directory of Open Access Journals (Sweden)

    Zhang Zhi-Ming

    2007-07-01

    Full Text Available Abstract Background Leptospirosis is a world-widely distributed zoonosis. Humans become infected via exposure to pathogenic Leptospira spp. from contaminated water or soil. The availability of genomic sequences of Leptospira interrogans serovar Lai and serovar Copenhageni opened up opportunities to identify genetic diversity among different pathogenic strains of L. interrogans representing various kinds of serotypes (serogroups and serovars. Results Comparative genomic hybridization (CGH analysis was used to compare the gene content of L. interrogans serovar Lai strain Lai with that of other 10 L. interrogans strains prevailed in China and one identified from Brazil using a microarray spotted with 3,528 protein coding sequences (CDSs of strain Lai. The cutoff ratio of sample/reference (S/R hybridization for detecting the absence of genes from one tested strain was set by comparing the ratio of S/R hybridization and the in silico sequence similarities of strain Lai and serovar Copenhageni strain Fiocruz L1-130. Among the 11 strains tested, 275 CDSs were found absent from at least one strain. The common backbone of the L. interrogans genome was estimated to contain about 2,917 CDSs. The genes encoding fundamental cellular functions such as translation, energy production and conversion were conserved. While strain-specific genes include those that encode proteins related to either cell surface structures or carbohydrate transport and metabolism. We also found two genomic islands (GIs in strain Lai containing genes divergently absent in other strains. Because genes encoding proteins with potential pathogenic functions are located within GIs, these elements might contribute to the variations in disease manifestation. Differences in genes involved in O-antigen biosynthesis were also identified for strains belonging to different serogroups, which offers an opportunity for future development of genomic typing tools for serological classification

  14. Transcriptional response of Leptospira interrogans to iron limitation and characterization of a PerR homolog

    Science.gov (United States)

    Leptospira interrogans is the causative agent of leptospirosis, a zoonosis of global significance. Iron is essential for growth of most bacterial species. Since availability of iron is low in the host, pathogens have evolved complex iron acquisition mechanisms to survive and establish infection. In ...

  15. Whole Genome Sequencing Allows Better Understanding of the Evolutionary History of Leptospira interrogans Serovar Hardjo

    Science.gov (United States)

    Llanes, Alejandro; Restrepo, Carlos Mario; Rajeev, Sreekumari

    2016-01-01

    The genome of a laboratory-adapted strain of Leptospira interrogans serovar Hardjo was sequenced and analyzed. Comparison of the sequenced genome with that recently published for a field isolate of the same serovar revealed relatively high sequence conservation at the nucleotide level, despite the different biological background of both samples. Conversely, comparison of both serovar Hardjo genomes with those of L. borgpetersenii serovar Hardjo showed extensive differences between the corresponding chromosomes, except for the region occupied by their rfb loci. Additionally, comparison of the serovar Hardjo genomes with those of different L. interrogans serovars allowed us to detect several genomic features that may confer an adaptive advantage to L. interrogans serovar Hardjo, including a possible integrated plasmid and an additional copy of a cluster encoding a membrane transport system known to be involved in drug resistance. A phylogenomic strategy was used to better understand the evolutionary position of the Hardjo serovar among L. interrogans serovars and other Leptospira species. The proposed phylogeny supports the hypothesis that the presence of similar rfb loci in two different species may be the result of a lateral gene transfer event. PMID:27442015

  16. Leptospira interrogans serovar hardjo in the kidneys and genital tracts of naturally infected sheep.

    Science.gov (United States)

    Cerri, D; Nuvoloni, R; Ebani, V; Pedrini, A; Mani, P; Andreani, E; Farina, R

    1996-04-01

    A bacteriological study was carried out to identify possible renal and/or genital carriers of Leptospira interrogans serovar hardjo. L. hardjo was found at slaughter in the kidneys of three seropositive ewes, but not in uterus or salpinges of these animals.

  17. Characterisation of the Rab binding properties of Rab coupling protein (RCP) by site-directed mutagenesis.

    Science.gov (United States)

    Lindsay, Andrew J; McCaffrey, Mary W

    2004-07-30

    Rab coupling protein (RCP) is a member of the Rab11-family of interacting proteins (Rab11-FIPs). Family members are characterised by their ability to interact with Rab11. This property is mediated by a conserved Rab binding domain (RBD) located at their carboxy-termini. Several Rab11-FIPs can also interact with other small GTPases. RCP interacts with Rab4 in addition to Rab11. To dissect out the individual properties of the Rab4 and Rab11 interactions with RCP, conserved amino acids within the RBD of RCP were mutated by site-directed mutagenesis. The effect of these mutations on Rab4 and Rab11 binding, and the intracellular localisation of RCP, was examined. Our results indicate that Rab11, rather than Rab4, mediates the intracellular localisation of RCP, and that the class I Rab11-FIPs compete for binding to Rab11.

  18. PENGGUNAAN KRITERIA rcP PADA PEMILIHAN PEUBAH BEBAS TERBAIK JIKA TERDAPAT MULTIKOLINEARITAS

    Directory of Open Access Journals (Sweden)

    Harmi Sugiarti

    2016-09-01

    Full Text Available Some procedures can be used for selecting independent variables, one of them is the procedure of all possible regression with robust Cp (RCp criterion. This statistic is not sensitive with multicollinearity in model and outlier residuals. The aim of this article is to investigate the use of RCp criterion in selecting independent variables. The result of the simulation experimental data shows that the RCp criterion fits enough to select independent variables.

  19. PENGGUNAAN KRITERIA rcP PADA PEMILIHAN PEUBAH BEBAS TERBAIK JIKA TERDAPAT MULTIKOLINEARITAS

    OpenAIRE

    Harmi Sugiarti

    2016-01-01

    Some procedures can be used for selecting independent variables, one of them is the procedure of all possible regression with robust Cp (RCp) criterion. This statistic is not sensitive with multicollinearity in model and outlier residuals. The aim of this article is to investigate the use of RCp criterion in selecting independent variables. The result of the simulation experimental data shows that the RCp criterion fits enough to select independent variables.

  20. Retrograde cerebral perfusion (RCP) in aortic arch surgery: efficacy and possible mechanisms of brain protection.

    Science.gov (United States)

    Bavaria, J E; Pochettino, A

    1997-07-01

    Retrograde cerebral perfusion (RCP) was first introduced to treat air embolism during cardiopulmonary bypass (CPB). Its use was reintroduced to extend the safety of hypothermic circulatory arrest (HCA) during operations involving an open aortic arch. RCP seems to prevent cerebral rewarming during HCA. Both clinical and animal data suggest that RCP provides between 10% and 30% of baseline cerebral blood flow when administered through the superior vena cava (SVC) at jugular pressures of 20 to 25 mm Hg. RCP flows producing jugular venous pressures higher than 30 mm Hg may cause cerebral edema. Cerebral blood flow generated by RCP is able to sustain some cerebral metabolic activity, yet is not able to fully meet cerebral energy demands even at temperatures of 12 degrees to 18 degrees C. RCP may further prevent embolic events during aortic arch surgery when administered at moderate jugular vein pressures (RCP, when applied during aortic arch reconstruction, may extend the safe HCA period and improve morbidity and mortality, especially when HCA times are more than 60 minutes. RCP applied in patients and severe carotid and brachiocephalic occlusive disease may be ineffective, and caution is in order when RCP times are greater than 90 minutes.

  1. 互联网络RCP(n)的组播路由算法%Study on Multicast Routing Algorithm of RCP(n) Interconnection Networks

    Institute of Scientific and Technical Information of China (English)

    刘宏英; 高太平

    2007-01-01

    互联网络RCP(Ringed Crossed cube Petersen)拓扑结构被提出后并没有给出组播算法.本文利用RCP(n)网络的正则性、良好的可扩展性,以及比Qn,HP(n),RHP(n)网络直径更短和构造开销更小这些特性,按照构造组播树的方法,给出了RCP(n)网络的组播路由算法,并对该算法作了性能分析.

  2. Identification of neotropical felid faeces using RCP-PCR.

    Science.gov (United States)

    Roques, S; Adrados, B; Chavez, C; Keller, C; Magnusson, W E; Palomares, F; Godoy, J A

    2011-01-01

    Faeces similarity among sympatric felid species has generally hampered their use in distributional, demographic and dietary studies. Here, we present a new and simple approach based on a set of species-specific primers, for the unambiguous identification of faeces from sympatric neotropical felids (i.e. puma, jaguar, jaguarundi and ocelot/ margay). This method, referred to as rapid classificatory protocol-PCR (RCP-PCR), consists of a single-tube multiplex PCR yielding species-specific banding patterns on agarose gel. The method was optimized with samples of known origin (14 blood and 15 fresh faeces) and validated in faecal samples of unknown origin (n = 138), for some of which (n = 40) we also obtained species identification based on mtDNA sequencing. This approach proved reliable and provides high identification success rates from faeces. Its simplicity and cost effectiveness should facilitate its application for routine surveys of presence and abundance of these species. © 2010 Blackwell Publishing Ltd.

  3. Ensemble simulations for the RCP8.5-Scenario

    Directory of Open Access Journals (Sweden)

    Friedrich-Wilhelm Gerstengarbe

    2015-04-01

    Full Text Available The mean climatic development for Germany was investigated within the period 2031/60 in comparison to the situation in the observational period 1981/2010. The RCP8.5-Scenario of the IPCC was used because it reflects the actual CO2-emissions very well. On this basis the temperature trend for Germany was estimated using 21 GCM runs up to the year 2100. This temperature trend was the driving force for the statistical regional climate model STARS. 100 ensemble runs of the model STARS were compared with the scenario period and with the observational period. Temperature, precipitation, climatic water balance and some additional parameters were analyzed. One important result is the change in the distribution of precipitation in Germany during the year – decrease in summer, increase in winter. Finally the future climate development leads to a negative climatic water balance over the whole year.

  4. In silico and microarray-based genomic approaches to identifying potential vaccine candidates against Leptospira interrogans

    Directory of Open Access Journals (Sweden)

    Jiang Xu-Cheng

    2006-11-01

    Full Text Available Abstract Background Currently available vaccines against leptospirosis are of low efficacy, have an unacceptable side-effect profile, do not induce long-term protection, and provide no cross-protection against the different serovars of pathogenic leptospira. The current major focus in leptospirosis research is to discover conserved protective antigens that may elicit longer-term protection against a broad range of Leptospira. There is a need to screen vaccine candidate genes in the genome of Leptospira interrogans. Results Bioinformatics, comparative genomic hybridization (CGH analysis and transcriptional analysis were used to identify vaccine candidates in the genome of L. interrogans serovar Lai strain #56601. Of a total of 4727 open reading frames (ORFs, 616 genes were predicted to encode surface-exposed proteins by P-CLASSIFIER combined with signal peptide prediction, α-helix transmembrane topology prediction, integral β-barrel outer membrane protein and lipoprotein prediction, as well as by retaining the genes shared by the two sequenced L. interrogans genomes and by subtracting genes with human homologues. A DNA microarray of L. interrogans strain #56601 was constructed for CGH analysis and transcriptome analysis in vitro. Three hundred and seven differential genes were identified in ten pathogenic serovars by CGH; 1427 genes had high transcriptional levels (Cy3 signal ≥ 342 and Cy5 signal ≥ 363.5, respectively. There were 565 genes in the intersection between the set encoding surface-exposed proteins and the set of 307 differential genes. The number of genes in the intersection between this set of 565 and the set of 1427 highly transcriptionally active genes was 226. These 226 genes were thus identified as putative vaccine candidates. The proteins encoded by these genes are not only potentially surface-exposed in the bacterium, but also conserved in two sequenced L. interrogans. Moreover, these genes are conserved among ten epidemic

  5. Characterization of the complement inhibitory function of rhesus rhadinovirus complement control protein (RCP).

    Science.gov (United States)

    Okroj, Marcin; Mark, Linda; Stokowska, Anna; Wong, Scott W; Rose, Nicola; Blackbourn, David J; Villoutreix, Bruno O; Spiller, O Brad; Blom, Anna M

    2009-01-02

    Rhesus rhadinovirus (RRV) is currently the closest known, fully sequenced homolog of human Kaposi sarcoma-associated herpesvirus. Both these viruses encode complement inhibitors as follows: Kaposi sarcoma-associated herpesvirus-complement control protein (KCP) and RRV-complement control protein (RCP). Previously we characterized in detail the functional properties of KCP as a complement inhibitor. Here, we performed comparative analyses for two variants of RCP protein, encoded by RRV strains H26-95 and 17577. Both RCP variants and KCP inhibited human and rhesus complement when tested in hemolytic assays measuring all steps of activation via the classical and the alternative pathway. RCP variants from both RRV strains supported C3b and C4b degradation by factor I and decay acceleration of the classical C3 convertase, similar to KCP. Additionally, the 17577 RCP variant accelerated decay of the alternative C3 convertase, which was not seen for KCP. In contrast to KCP, RCP showed no affinity to heparin and is the first described complement inhibitor in which the binding site for C3b/C4b does not interact with heparin. Molecular modeling shows a structural disruption in the region of RCP that corresponds to the KCP-heparin-binding site. This makes RRV a superior model for future in vivo investigations of complement evasion, as RCP does not play a supportive role in viral attachment as KCP does.

  6. European School of Internal Medicine: a window of opportunity for RCP activities in Europe.

    Science.gov (United States)

    Davidson, Chris; Higgens, Clare

    2009-04-01

    The Royal College of Physicians (RCP) is to host the European School of Internal Medicine for two years from 2009-10. This affords a unique opportunity for specialist registrars to exchange ideas about professional development and training and to make contacts with young internists from across Europe. Such links should prove useful for future RCP initiatives in European medicine.

  7. Risk Analyses of Charging Pump Control Improvements for Alternative RCP Seal Cooling

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Eun-Chan [Korea Hydro and Nuclear Power Co. Ltd. Daejeon (Korea, Republic of)

    2015-10-15

    There are two events that significantly affect the plant risk during a TLOCCW event. One is an event in which the seal assembly of a reactor coolant pump (RCP) fails due to heating stress from the loss of cooling water; the other is an event in which the operators fail to conduct alternative cooling for the RCP seal during the accident. KHNP reviewed the replacement of the RCP seal with a qualified shutdown seal in order to remove the risk due to RCP seal failure during a TLOCCW. As an optional measure, a design improvement in the alternative cooling method for the RCP seal is being considered. This analysis presents the alternative RCP seal cooling improvement and its safety effect. K2 is a nuclear power plant with a Westinghouse design, and it has a relatively high CDF during TLOCCW events because it has a different CCW system design and difficulty in preparing alternative cooling water sources. This analysis confirmed that an operator action providing cold water to the RWST as RCP seal injection water during a TLOCCW event is very important in K2. The control circuit improvement plan for the auxiliary charging pump was established in order to reduce the failure probability of this operator action. This analysis modeled the improvement as a fault tree and evaluated the resulting CDF change. The consequence demonstrated that the RCP seal injection failure probability was reduced by 89%, and the CDF decreased by 28%.

  8. The role of the CGRP-receptor component protein (RCP) in adrenomedullin receptor signal transduction.

    Science.gov (United States)

    Prado, M A; Evans-Bain, B; Oliver, K R; Dickerson, I M

    2001-11-01

    G protein-coupled receptors are usually thought to act as monomer receptors that bind ligand and then interact with G proteins to initiate signal transduction. In this study we report an intracellular peripheral membrane protein named the calcitonin gene-related peptide (CGRP)-receptor component protein (RCP) required for signal transduction at the G protein-coupled receptor for adrenomedullin. Cell lines were made that expressed an antisense construct of the RCP cDNA, and in these cells diminished RCP expression correlated with loss of adrenomedullin signal transduction. In contrast, loss of RCP did not diminish receptor density or affinity, therefore RCP does not appear to act as a chaperone protein. Instead, RCP represents a novel class of protein required to couple the adrenomedullin receptor to the cellular signal transduction pathway. A candidate adrenomedullin receptor named the calcitonin receptor-like receptor (CRLR) has been described, which forms high affinity adrenomedullin receptors when co-expressed with the accessory protein receptor-activity modifying protein 2 (RAMP2). RCP co-immunoprecipitated with CRLR and RAMP2, indicating that a functional adrenomedullin receptor is composed of at least three proteins: the ligand binding protein (CRLR), an accessory protein (RAMP2), and a coupling protein for signal transduction (RCP).

  9. Data on Leptospira interrogans sv Pomona infection in Meat Workers in New Zealand.

    Science.gov (United States)

    Pittavino, M; Dreyfus, A; Heuer, C; Benschop, J; Wilson, P; Collins-Emerson, J; Torgerson, P R; Furrer, R

    2017-08-01

    The data presented in this article are related to the research article entitled "Comparison between Generalized Linear Modelling and Additive Bayesian Network; Identification of Factors associated with the Incidence of Antibodies against Leptospira interrogans sv Pomona in Meat Workers in New Zealand" (Pittavino et al., 2017) [5]. A prospective cohort study was conducted in four sheep slaughtering abattoirs in New Zealand (NZ) (Dreyfus et al., 2015) [1]. Sera were collected twice a year from 384 meat workers and tested by Microscopic Agglutination for Leptospira interrogans sv Pomona (Pomona) infection, one of the most common Leptospira serovars in humans in NZ. This article provides an extended analysis of the data, illustrating the different steps of a multivariable (i.e. generalized linear model) and especially a multivariate tool based on additive Bayesian networks (ABN) modelling.

  10. Leptospira interrogans serovar Copenhageni Harbors Two lexA Genes Involved in SOS Response

    Science.gov (United States)

    Fonseca, Luciane S.; da Silva, Josefa B.; Milanez, Juliana S.; Monteiro-Vitorello, Claudia B.; Momo, Leonardo; de Morais, Zenaide M.; Vasconcellos, Silvio A.; Marques, Marilis V.; Ho, Paulo L.; da Costa, Renata M. A.

    2013-01-01

    Bacteria activate a regulatory network in response to the challenges imposed by DNA damage to genetic material, known as the SOS response. This system is regulated by the RecA recombinase and by the transcriptional repressor lexA. Leptospira interrogans is a pathogen capable of surviving in the environment for weeks, being exposed to a great variety of stress agents and yet retaining its ability to infect the host. This study aims to investigate the behavior of L. interrogans serovar Copenhageni after the stress induced by DNA damage. We show that L. interrogans serovar Copenhageni genome contains two genes encoding putative LexA proteins (lexA1 and lexA2) one of them being potentially acquired by lateral gene transfer. Both genes are induced after DNA damage, but the steady state levels of both LexA proteins drop, probably due to auto-proteolytic activity triggered in this condition. In addition, seven other genes were up-regulated following UV-C irradiation, recA, recN, dinP, and four genes encoding hypothetical proteins. This set of genes is potentially regulated by LexA1, as it showed binding to their promoter regions. All these regions contain degenerated sequences in relation to the previously described SOS box, TTTGN 5CAAA. On the other hand, LexA2 was able to bind to the palindrome TTGTAN 10TACAA, found in its own promoter region, but not in the others. Therefore, the L. interrogans serovar Copenhageni SOS regulon may be even more complex, as a result of LexA1 and LexA2 binding to divergent motifs. New possibilities for DNA damage response in Leptospira are expected, with potential influence in other biological responses such as virulence. PMID:24098496

  11. Leptospira interrogans serovar copenhageni harbors two lexA genes involved in SOS response.

    Science.gov (United States)

    Fonseca, Luciane S; da Silva, Josefa B; Milanez, Juliana S; Monteiro-Vitorello, Claudia B; Momo, Leonardo; de Morais, Zenaide M; Vasconcellos, Silvio A; Marques, Marilis V; Ho, Paulo L; da Costa, Renata M A

    2013-01-01

    Bacteria activate a regulatory network in response to the challenges imposed by DNA damage to genetic material, known as the SOS response. This system is regulated by the RecA recombinase and by the transcriptional repressor lexA. Leptospira interrogans is a pathogen capable of surviving in the environment for weeks, being exposed to a great variety of stress agents and yet retaining its ability to infect the host. This study aims to investigate the behavior of L. interrogans serovar Copenhageni after the stress induced by DNA damage. We show that L. interrogans serovar Copenhageni genome contains two genes encoding putative LexA proteins (lexA1 and lexA2) one of them being potentially acquired by lateral gene transfer. Both genes are induced after DNA damage, but the steady state levels of both LexA proteins drop, probably due to auto-proteolytic activity triggered in this condition. In addition, seven other genes were up-regulated following UV-C irradiation, recA, recN, dinP, and four genes encoding hypothetical proteins. This set of genes is potentially regulated by LexA1, as it showed binding to their promoter regions. All these regions contain degenerated sequences in relation to the previously described SOS box, TTTGN 5CAAA. On the other hand, LexA2 was able to bind to the palindrome TTGTAN10TACAA, found in its own promoter region, but not in the others. Therefore, the L. interrogans serovar Copenhageni SOS regulon may be even more complex, as a result of LexA1 and LexA2 binding to divergent motifs. New possibilities for DNA damage response in Leptospira are expected, with potential influence in other biological responses such as virulence.

  12. Leptospira interrogans serovar copenhageni harbors two lexA genes involved in SOS response.

    Directory of Open Access Journals (Sweden)

    Luciane S Fonseca

    Full Text Available Bacteria activate a regulatory network in response to the challenges imposed by DNA damage to genetic material, known as the SOS response. This system is regulated by the RecA recombinase and by the transcriptional repressor lexA. Leptospira interrogans is a pathogen capable of surviving in the environment for weeks, being exposed to a great variety of stress agents and yet retaining its ability to infect the host. This study aims to investigate the behavior of L. interrogans serovar Copenhageni after the stress induced by DNA damage. We show that L. interrogans serovar Copenhageni genome contains two genes encoding putative LexA proteins (lexA1 and lexA2 one of them being potentially acquired by lateral gene transfer. Both genes are induced after DNA damage, but the steady state levels of both LexA proteins drop, probably due to auto-proteolytic activity triggered in this condition. In addition, seven other genes were up-regulated following UV-C irradiation, recA, recN, dinP, and four genes encoding hypothetical proteins. This set of genes is potentially regulated by LexA1, as it showed binding to their promoter regions. All these regions contain degenerated sequences in relation to the previously described SOS box, TTTGN 5CAAA. On the other hand, LexA2 was able to bind to the palindrome TTGTAN10TACAA, found in its own promoter region, but not in the others. Therefore, the L. interrogans serovar Copenhageni SOS regulon may be even more complex, as a result of LexA1 and LexA2 binding to divergent motifs. New possibilities for DNA damage response in Leptospira are expected, with potential influence in other biological responses such as virulence.

  13. Leptospira interrogans endostatin-like outer membrane proteins bind host fibronectin, laminin and regulators of complement.

    Directory of Open Access Journals (Sweden)

    Brian Stevenson

    Full Text Available The pathogenic spirochete Leptospira interrogans disseminates throughout its hosts via the bloodstream, then invades and colonizes a variety of host tissues. Infectious leptospires are resistant to killing by their hosts' alternative pathway of complement-mediated killing, and interact with various host extracellular matrix (ECM components. The LenA outer surface protein (formerly called LfhA and Lsa24 was previously shown to bind the host ECM component laminin and the complement regulators factor H and factor H-related protein-1. We now demonstrate that infectious L. interrogans contain five additional paralogs of lenA, which we designated lenB, lenC, lenD, lenE and lenF. All six genes encode domains predicted to bear structural and functional similarities with mammalian endostatins. Sequence analyses of genes from seven infectious L. interrogans serovars indicated development of sequence diversity through recombination and intragenic duplication. LenB was found to bind human factor H, and all of the newly-described Len proteins bound laminin. In addition, LenB, LenC, LenD, LenE and LenF all exhibited affinities for fibronectin, a distinct host extracellular matrix protein. These characteristics suggest that Len proteins together facilitate invasion and colonization of host tissues, and protect against host immune responses during mammalian infection.

  14. A dominant clone of Leptospira interrogans associated with an outbreak of human leptospirosis in Thailand.

    Science.gov (United States)

    Thaipadungpanit, Janjira; Wuthiekanun, Vanaporn; Chierakul, Wirongrong; Smythe, Lee D; Petkanchanapong, Wimol; Limpaiboon, Roongrueng; Apiwatanaporn, Apichat; Slack, Andrew T; Suputtamongkol, Yupin; White, Nicholas J; Feil, Edward J; Day, Nicholas P J; Peacock, Sharon J

    2007-10-31

    A sustained outbreak of leptospirosis occurred in northeast Thailand between 1999 and 2003, the basis for which was unknown. A prospective study was conducted between 2000 and 2005 to identify patients with leptospirosis presenting to Udon Thani Hospital in northeast Thailand, and to isolate the causative organisms from blood. A multilocus sequence typing scheme was developed to genotype these pathogenic Leptospira. Additional typing was performed for Leptospira isolated from human cases in other Thai provinces over the same period, and from rodents captured in the northeast during 2004. Sequence types (STs) were compared with those of Leptospira drawn from a reference collection. Twelve STs were identified among 101 isolates from patients in Udon Thani. One of these (ST34) accounted for 77 (76%) of isolates. ST34 was Leptospira interrogans, serovar Autumnalis. 86% of human Leptospira isolates from Udon Thani corresponded to ST34 in 2000/2001, but this figure fell to 56% by 2005 as the outbreak waned (p = 0.01). ST34 represented 17/24 (71%) of human isolates from other Thai provinces, and 7/8 (88%) rodent isolates. By contrast, 59 STs were found among 76 reference strains, indicating a much more diverse population genetic structure; ST34 was not identified in this collection. Development of an MLST scheme for Leptospira interrogans revealed that a single ecologically successful pathogenic clone of L. interrogans predominated in the rodent population, and was associated with a sustained outbreak of human leptospirosis in Thailand.

  15. A dominant clone of Leptospira interrogans associated with an outbreak of human leptospirosis in Thailand.

    Directory of Open Access Journals (Sweden)

    Janjira Thaipadungpanit

    2007-10-01

    Full Text Available A sustained outbreak of leptospirosis occurred in northeast Thailand between 1999 and 2003, the basis for which was unknown.A prospective study was conducted between 2000 and 2005 to identify patients with leptospirosis presenting to Udon Thani Hospital in northeast Thailand, and to isolate the causative organisms from blood. A multilocus sequence typing scheme was developed to genotype these pathogenic Leptospira. Additional typing was performed for Leptospira isolated from human cases in other Thai provinces over the same period, and from rodents captured in the northeast during 2004. Sequence types (STs were compared with those of Leptospira drawn from a reference collection. Twelve STs were identified among 101 isolates from patients in Udon Thani. One of these (ST34 accounted for 77 (76% of isolates. ST34 was Leptospira interrogans, serovar Autumnalis. 86% of human Leptospira isolates from Udon Thani corresponded to ST34 in 2000/2001, but this figure fell to 56% by 2005 as the outbreak waned (p = 0.01. ST34 represented 17/24 (71% of human isolates from other Thai provinces, and 7/8 (88% rodent isolates. By contrast, 59 STs were found among 76 reference strains, indicating a much more diverse population genetic structure; ST34 was not identified in this collection.Development of an MLST scheme for Leptospira interrogans revealed that a single ecologically successful pathogenic clone of L. interrogans predominated in the rodent population, and was associated with a sustained outbreak of human leptospirosis in Thailand.

  16. RCP induces Slug expression and cancer cell invasion by stabilizing β1 integrin.

    Science.gov (United States)

    Hwang, M H; Cho, K H; Jeong, K J; Park, Y-Y; Kim, J M; Yu, S-L; Park, C G; Mills, G B; Lee, H Y

    2017-02-23

    Rab coupling protein (RCP)-induced tumor cell migration has been implicated in tumor pathophysiology and patient outcomes. In the present study, we demonstrate that RCP stabilizes β1 integrin leading to increased β1 integrin levels and activation of a signaling cascade culminating in Slug induction, epithelial-to-mesenchymal transition and increased invasion. Ectopic expression of RCP induced Slug expression. Silencing β1 integrin efficiently inhibited RCP-induced Slug expression and subsequent cancer cell invasion. Conversely, ectopic expression of β1 integrin was sufficient to induce Slug expression. Pharmacological inhibition of integrin linked kinase (ILK), EGFR and NF-κB, as well as transfection of a dominant-negative mutant of Ras (RasN17), significantly inhibited RCP-induced Slug expression and cancer cell invasion. Strikingly, ectopic expression of RCP was sufficient to enhance metastasis of ovarian cancer cells to the lung. Collectively, we demonstrate a mechanism by which RCP promotes cancer cell aggressiveness through sequential β1 integrin stabilization, activation of an ILK/EGFR/Ras/NF-κB signaling cascade and subsequent Slug expression.

  17. Prospects of riboflavin carrier protein (RCP) as an antifertility vaccine in male and female mammals.

    Science.gov (United States)

    Adiga, P R; Subramanian, S; Rao, J; Kumar, M

    1997-01-01

    Riboflavin carrier protein (RCP) is obligatorily involved in yolk deposition of the vitamin, riboflavin, in the developing oocyte of the hen. The production of this protein is inducible by oestrogen. It is evolutionarily conserved in terms of its physicochemical, immunological and functional characteristics. It is the prime mediator of vitamin supply to the developing fetus in mammals, including primates. Passive immunoneutralization of the protein terminates pregnancy in rats. Active immunization of rats and bonnet monkeys with avian RCP prevents pregnancy without causing any adverse physiological effects of the mother in terms of her vitamin status, reproductive cycles or reproductive-endocrine profile. Denatured, linearized RCP is more effective in eliciting neutralizing antibodies capable of interfering with embryonic viability either before or during peri-implantation stages. Two defined stretches of sequential epitopes, one located at the N-terminus and the other at the C-terminus of the protein have been identified. Active immunization with either of these epitopes conjugated with diphtheria toxoid curtails pregnancy in rats and monkeys. Immunohistochemical localization of RCP on ovulated oocytes and early embryos shows that the antibodies cause degeneration only of early embryos. RCP is produced intra-testicularly and becomes localized on acrosomal surface of mammalian spermatozoa. Active immunization of male rats and monkeys with denatured RCP markedly reduces fertility by impairing the fertilizing potential of spermatozoa. These findings suggest that RCP, or its defined fragments, could be a novel, first generation vaccine for regulating fertility in both the sexes.

  18. A new fluorescent imaging of renal inflammation with RCP.

    Science.gov (United States)

    Nakamura, Kentaro; Tabata, Yasuhiko

    2010-12-20

    The objective of this study is to design a fluorescent imaging agent with R-Gel, one of the recombinant polymers (RCP), for renal inflammation. The R-Gel based on human type I collagen has multiple Arg-Gly-Asp (RGD) motifs which are ligands for some types of integrin receptors on the cell surface. After intravenous administration of R-Gel labeled by Cy7 of a fluorescent dye to three animal models of nephritis mousse, interstitial nephritis (by using UUO model mice), glomerulonephritis (HIGA mice), and ischemia-reperfusion injured kidney (I/R mice), the extent of fluorescent imaging at the renal inflammation was assessed. The Cy7-labeled R-Gel was accumulated in the inflammation site to a significantly greater extent than in the normal one at 24h after administration. The renal pattern of fluorescent imaging was similar to that of administration anti-Mac1 antibody. Taken together, it is conceivable that the R-Gel was targeted to macrophages infiltrated into the inflammation site of kidney. Copyright © 2010 Elsevier B.V. All rights reserved.

  19. Optimization Design of Topological Structure on Interconnection Networks——RCP(n)%互联网络RCP(n)的拓扑结构优化设计

    Institute of Scientific and Technical Information of China (English)

    刘三满

    2008-01-01

    提出一种新的互联网络拓扑结构--基于交叉立方体环连接的Petersen图互联网络RCP(n).研究互联网络RCP(n)的通信特性.通过RCP(n)的单播路由算法、广播路由算法、可分组性算法,证明RCP(n)不仅具有环、彼特森图和交叉立方体本身所具有的性质,同时又具有自身独特的拓扑性质.研究结果表明,RCP(n)是一种具有良好拓扑结构和通信特性的互联网络.在通信效率上的花费只有由超立方体构成的互联网络的1/2,而通信效率却是由超立方体构成的互联网络的一倍.

  20. Is Rab25 a tumor promoter or suppressor--context dependency on RCP status?

    Science.gov (United States)

    Tang, Bor Luen

    2010-08-01

    Conflicting reports in the literature suggest that Rab25 could either be a context dependent promoter or suppressor of tumorigenesis. We hypothesized that whether Rab25 acts as a promoter or suppressor in tumor progression depends on the expression status of its effector, the Rab coupling protein (RCP). An elevated expression of RCP resulting from genomic amplification may enhance Rab25's tumor progression activity. Elevation of Rab25 alone may sequester endogenous RCP, and attenuates its activating effect on other oncogenic products, such as mutant Ras.

  1. Guías clinicas de RCP y SRI enfermería

    OpenAIRE

    Donis Mulero, Elena

    2015-01-01

    La reanimación cardiopulmonar (RCP) es un procedimiento de emergencia para salvar vidas que se lleva a cabo cuando una persona se encuentra en parada cardiorrespiratoria (PCR). Tanto la técnica de RCP como la de intubación, son aspectos olvidados por los profesionales de la salud. Recopilar las últimas recomendaciones sobre RCP para poder elaborar un programa sencillo enfocado a la enfermería. Elaborar una guía sencilla de secuencia rápida de intubación (SRI). Revisión bibliográfica en la que...

  2. 互联网络RCP(n)的组播路由算法

    Institute of Scientific and Technical Information of China (English)

    刘宏英; 高太平

    2007-01-01

    互联网络RCP(Ringed Crossed cube Petersen)拓扑结构被提出后并没有给出组播算法.本文利用RCP(n)网络的正则性、良好的可扩展性,以及比Qn,HP(n),RHP(n)网络直径更短和构造开销更小这些特性,按照构造组播树的方法,给出了RCP(n)网络的组播路由算法,并对该算法作了性能分析.

  3. Identification and classification of all potential hemolysin encoding genes and their products from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai

    Institute of Scientific and Technical Information of China (English)

    Yi-xuan ZHANG; Yan GENG; Bo BI; Jian-yong HE; Chun-fu WU; Xiao-kui GUO; Guo-ping ZHAO

    2005-01-01

    Aim: To identify and classify all potential hemolysin candidates of Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai. Methods: All of thepotential hemolysin encoding genes were characterized in silico. These genes were cloned and expressed in Escherichia coli. The hemolytic activities of the expressed proteins were assayed observing the hemolysis on sheep blood agar plates. Sphingomyelinase activities of the hemolysin candidates were measured by thin-layer chromatography (TLC) and HPLC for sphingomyelin-hydrolysis. Expression and secretion of the hemolysins in L interrogans were studied by reverse transcription polymerase chain reaction, Western blot, and enzyme-linked immunosorbent assays. Results and Conclusion: The hemolytic activities of hemolysin candidates (LA0327, LA0378, LA1027, LA1029, LA1650, LA3050,LA3937, LA4004) from L interrogans strain Lai were confirmed. They were further divided into two groups, sphingomyelinase hemolysins and non-sphingomyelinase hemolysins, based on their ability to hydrolyze sphingomyelin. Most of these hemolysins were actually expressed in living L interrogans and some of them were secreted into the environment. This study establishes an essential and complete basis for further studying the contribution of hemolysins to the pathogenesis of L interrogans.

  4. Seroprevalence and risk factors associated with within-flock transmission of Leptospira interrogans in transhumant farming systems in Mexico.

    Science.gov (United States)

    Arteaga-Troncoso, G; Jiménez-Estrada, J M; Montes De Oca-Jimenez, R; López-Hurtado, M; Luna-Alvarez, M; Hernandez-Andrade, L; Moreno-Alfaro, A; Galan-Herrera, J F; Guerra-Infante, F M

    2015-10-01

    A number of recent reports emphasize the risk of zoonotic diseases and the high degree of prevalence of asymptomatic animals infected with Leptospira interrogans. This report sought to assess the prevalence of antibodies to certain serovars of L. interrogans, and to describe the association between seropositivity and risk factors associated with within-flock transmission in a mountainous region of Mexico. Overall seroprevalence to L. interrogans was 54·5% (95% confidence interval 48·3-60·7); the most frequent serovar was Icterohaemorrhagiae. The accumulation of placentas and fetuses at a site close to lambing paddocks can play a significant role as a risk factor for within-flock transmission of L. interrogans in transhumant farming systems in the municipality of Xalatlaco. The high prevalence of L. interrogans antibodies supports the hypothesis that natural foci of this zoonosis are present in sheep flocks in this area. These findings emphasize the need for planning and implementation of control programmes for ovine leptospirosis in Mexico and elsewhere.

  5. Analysing the climatic extremes of future projections for the MedCORDEX domain using RCP4.5 and RCP8.5 scenario

    Science.gov (United States)

    Bartholy, Judit; Pongracz, Rita; Pieczka, Ildiko; Szabone Andre, Karolina

    2017-04-01

    In this study HadGEM2 global climate model outputs were downscaled with RegCM4.3 for the entire MED-44 CORDEX area for the period 1950-2099 using RCP4.5 and RCP8.5 scenario. The 50-km resolution RegCM-outputs served as input for further downscaling using 10 km as a horizontal resolution for a smaller domain covering Central Europe with special focus on the Carpathian Region. RCP4.5 is a stabilization scenario while RCP8.5 is a rising radiative forcing pathway, therefore, the difference in the simulation outputs helps to quantify the inertia of the climate system, the importance of anthropogenic influence on climate, and shows the evidence for the need of mitigation and adaptation measures. Evidently, higher temperature change corresponds to RCP8.5 compared to RCP4.5. The difference of global and/or regional warming between the two scenario can reach (or even exceed) 2 °C from the second part of the century. Differences in precipitation projections are less straightforward to explain as no direct link exists with warming and radiative forcing, however, the annual distribution of precipitation is projected to change, which may lead to important consequences on society. Our analysis compares the estimated temperature and precipitation changes with special focus on extreme climatic conditions for the following 10 subregions of the MED-44 CORDEX area: Iberian Peninsula, Apennine Peninsula, Balkan Region, Asia Minor, East European Plain, Middle European Plain, Carpathian Basin, Carpathian Mountains, Alps, Western Europe.

  6. Detección de proteínas de adhesión a fibronectina y colágeno presentes en Leptospira interrogans serovar Canicola

    Directory of Open Access Journals (Sweden)

    Gisele Reyes

    2007-12-01

    Full Text Available Como parte de los estudios encaminados a la obtención de una formulación vacunal por subunidades contra la leptospirosis humana, se describe la purificación y caracterización de las proteínas de unión a fibronectina y a colágeno en Leptospira interrogans. Las proteínas de la membrana externa fueron extraídas mediante la solubilización con Tritón X-114 y se aplicaron en una columna de afinidad de IgG AntiBSA-Sepharose 2B CL, para eliminar la BSA, contaminante principal del medio de cultivo en que crece el microorganismo. La muestra libre de BSA (no fijado se aplicó a una columna de afinidad de fibronectina Sepharose 4B-CNBr, que permitió la separación y detección de una fracción que contenía una proteína de unión a fibronectina presente en la cepa 87 de Leptospira interrogans serovar Canicola, cuyo peso molecular fue estimado en 40 kDa. La proteína aislada demostró ser antigénica y conservada en los serovares Canicola, Copenhageni y Mozdok, en el ensayo de inmunodetección utilizado en este estudio (Dot blot. Para ello se utilizaron sueros específicos obtenidos en ratas infectadas experimentalmente con cada serovar y una mezcla de sueros de humanos convalecientes de leptospirosis. Las proteínas de membrana externa solubilizadas con Tritón X-114, libres de BSA, fueron aplicadas también a una columna de afinidad colágeno-Sepharosa 4B-CNBr, que permitió la purificación de una proteína de unión a colágeno con un peso molecular de aproximadamente 25 kDa, la cual resultó ser antigénica frente a sueros de humanos convalecientes de la enfermedad. Ambas proteínas seleccionadas (40 kD y 25 kD podrían ser evaluadas como posibles inmunógenos en futuros estudios encaminados a la obtención de nuevos antígenos vacunales.

  7. Diacylglycerol kinase α controls RCP-dependent integrin trafficking to promote invasive migration.

    Science.gov (United States)

    Rainero, Elena; Caswell, Patrick T; Muller, Patricia A J; Grindlay, Joan; McCaffrey, Mary W; Zhang, Qifeng; Wakelam, Michael J O; Vousden, Karen H; Graziani, Andrea; Norman, Jim C

    2012-01-23

    Inhibition of αvβ3 integrin or expression of oncogenic mutants of p53 promote invasive cell migration by enhancing endosomal recycling of α5β1 integrin under control of the Rab11 effector Rab-coupling protein (RCP). In this paper, we show that diacylglycerol kinase α (DGK-α), which phosphorylates diacylglycerol to phosphatidic acid (PA), was required for RCP to be mobilized to and tethered at the tips of invasive pseudopods and to allow RCP-dependent α5β1 recycling and the resulting invasiveness of tumor cells. Expression of a constitutive-active mutant of DGK-α drove RCP-dependent invasion in the absence of mutant p53 expression or αvβ3 inhibition, and conversely, an RCP mutant lacking the PA-binding C2 domain was not capable of being tethered at pseudopod tips. These data demonstrate that generation of PA downstream of DGK-α is essential to connect expression of mutant p53s or inhibition of αvβ3 to RCP and for this Rab11 effector to drive the trafficking of α5β1 that is required for tumor cell invasion through three-dimensional matrices.

  8. Shortest path algorithm of RCP(n) networks%互联网络RCP(n)的最短路算法

    Institute of Scientific and Technical Information of China (English)

    王敏; 高太平; 刘宏英; 闫宇琦

    2009-01-01

    RCP(n)是最近提出的一种新型互联网络拓扑结构,是由环、Petersen图和交叉立方体所组成的,具有短直径、良好的可扩展性和正则性以及较小的构造开销的性质,是一种具有良好拓扑性质的互联网络.针对RCP(n)上节点编码的特点,采用逐步分解编码,依次寻找路径的方法给出了寻找RCP(n)上任意两点间最短路的一个多项式算法,为RCP(n)上作进一步的路由算法、最优分组等通讯性能的研究提供了理论支持,因此具有一定的理论意义和应用价值.

  9. Data on Leptospira interrogans sv Pomona infection in Meat Workers in New Zealand

    Directory of Open Access Journals (Sweden)

    M. Pittavino

    2017-08-01

    A prospective cohort study was conducted in four sheep slaughtering abattoirs in New Zealand (NZ (Dreyfus et al., 2015 [1]. Sera were collected twice a year from 384 meat workers and tested by Microscopic Agglutination for Leptospira interrogans sv Pomona (Pomona infection, one of the most common Leptospira serovars in humans in NZ. This article provides an extended analysis of the data, illustrating the different steps of a multivariable (i.e. generalized linear model and especially a multivariate tool based on additive Bayesian networks (ABN modelling.

  10. Characterization and localization of the rabbit ocular calcitonin gene-related peptide (CGRP)-receptor component protein (RCP).

    Science.gov (United States)

    Rosenblatt, M I; Dahl, G P; Dickerson, I M

    2000-04-01

    To determine whether the calcitonin gene-related peptide (CGRP) receptor component protein (RCP), a novel signal transduction molecule, is required for CGRP signaling in the eye and to determine potential ocular sites of CGRP action. The cDNA for the rabbit ocular RCP homologue was cloned using a combination of reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). Function of the rabbit ocular RCP was assessed using a sensitive oocyte-based assay, which utilizes the protein kinase A (PKA)-sensitive cystic fibrosis transmembrane conductance regulator (CFTR) as a sensor of cAMP formation. RCP expression in the rabbit eye was localized using immunohistochemistry. A 2063-bp cDNA for the rabbit ocular RCP was cloned and sequenced. Expression of the rabbit RCP cDNA confers CGRP responsiveness in a sensitive oocyte-based assay. Antisense oligonucleotides made to the ocular RCP abolishes CGRP responsiveness of ciliary body and iris mRNA in the oocyte-CFTR assay. Localization of RCP protein in the rabbit eye using immunohistochemistry demonstrated RCP immunoreactivity in the ciliary body and iris blood vessels, as well as in layers of the ciliary epithelium. The rabbit ocular RCP appears to be required for signal transduction at ocular CGRP receptors and is localized to sites previously reported to bind CGRP, which affect intraocular pressure and neurogenic inflammation.

  11. Sensitivity Analysis on LOCCW of Westinghouse typed Reactors Considering WOG2000 RCP Seal Leakage Model

    Energy Technology Data Exchange (ETDEWEB)

    Na, Jang-Hwan; Jeon, Ho-Jun; Hwang, Seok-Won [KHNP Central Research Institute, Daejeon (Korea, Republic of)

    2015-10-15

    In this paper, we focus on risk insights of Westinghouse typed reactors. We identified that Reactor Coolant Pump (RCP) seal integrity is the most important contributor to Core Damage Frequency (CDF). As we reflected the latest technical report; WCAP-15603(Rev. 1-A), 'WOG2000 RCP Seal Leakage Model for Westinghouse PWRs' instead of the old version, RCP seal integrity became more important to Westinghouse typed reactors. After Fukushima accidents, Korea Hydro and Nuclear Power (KHNP) decided to develop Low Power and Shutdown (LPSD) Probabilistic Safety Assessment (PSA) models and upgrade full power PSA models of all operating Nuclear Power Plants (NPPs). As for upgrading full power PSA models, we have tried to standardize the methodology of CCF (Common Cause Failure) and HRA (Human Reliability Analysis), which are the most influential factors to risk measures of NPPs. Also, we have reviewed and reflected the latest operating experiences, reliability data sources and technical methods to improve the quality of PSA models. KHNP has operating various types of reactors; Optimized Pressurized Reactor (OPR) 1000, CANDU, Framatome and Westinghouse. So, one of the most challengeable missions is to keep the balance of risk contributors of all types of reactors. This paper presents the method of new RCP seal leakage model and the sensitivity analysis results from applying the detailed method to PSA models of Westinghouse typed reference reactors. To perform the sensitivity analysis on LOCCW of the reference Westinghouse typed reactors, we reviewed WOG2000 RCP seal leakage model and developed the detailed event tree of LOCCW considering all scenarios of RCP seal failures. Also, we performed HRA based on the T/H analysis by using the leakage rates for each scenario. We could recognize that HRA was the sensitive contributor to CDF, and the RCP seal failure scenario of 182gpm leakage rate was estimated as the most important scenario.

  12. Evaluation of Pathogenic Serovars of Leptospira Interrogans in Dairy Cattle Herds of Shahrekord by PCR

    Directory of Open Access Journals (Sweden)

    HR Shahbazkia

    2011-12-01

    Full Text Available Background and objectives: Leptospirosis is an important zoonotic disease caused by Leptospira interrogans. Leptospirosis leads to economical losses in dairy farm industry. The objective of this study was to evaluate the pathogenic serovars of Leptospira interrogans in dairy cattle herds of Shahrekord by PCR.Materials and Methods: Two hundred samples (100 urine and 100 blood were collected from 100 cows randomly and delivered to the laboratory. Samples were stored at -20 °C. DNA was extracted and purified from the plasma and urine samples and concentrated on diatoms in the presence of guanidine thiocyanate (GuSCN. PCR products were detected and identified as Leptospira by ilumination of the expected size of DNA bands after staining of the agarose gel with ethidium bromide gels. PCR products were purified and sequenced.Results: The results showed that 28% of urine samples and 23% of plasma samples were contaminated. The major serotypes were Icterohaemorrhagiae (50% and Pomona (37.5%. The urine samples of 17 cows were positive for Leptospira without positive plasma samples. This indicated that these cows are reservoirs in dairy herds of Shahrekord and dangerous for human health. The plasma samples of twelve cows were positive for Leptospira without positive urine samples.Conclusions: Leptospira serotypes can be maintained in relatively dry regions and must be considered when dealing with leptospirosis in dairy farms of Shahrekord and human health.

  13. A LigA three-domain region protects hamsters from lethal infection by Leptospira interrogans.

    Directory of Open Access Journals (Sweden)

    Mariana L Coutinho

    2011-12-01

    Full Text Available The leptospiral LigA protein consists of 13 bacterial immunoglobulin-like (Big domains and is the only purified recombinant subunit vaccine that has been demonstrated to protect against lethal challenge by a clinical isolate of Leptospira interrogans in the hamster model of leptospirosis. We determined the minimum number and location of LigA domains required for immunoprotection. Immunization with domains 11 and 12 was found to be required but insufficient for protection. Inclusion of a third domain, either 10 or 13, was required for 100% survival after intraperitoneal challenge with Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130. As in previous studies, survivors had renal colonization; here, we quantitated the leptospiral burden by qPCR to be 1.2×10(3 to 8×10(5 copies of leptospiral DNA per microgram of kidney DNA. Although renal histopathology in survivors revealed tubulointerstitial changes indicating an inflammatory response to the infection, blood chemistry analysis indicated that renal function was normal. These studies define the Big domains of LigA that account for its vaccine efficacy and highlight the need for additional strategies to achieve sterilizing immunity to protect the mammalian host from leptospiral infection and its consequences.

  14. Characterization of a bifunctional enzyme with (p)ppGpp-hydrolase/synthase activity in Leptospira interrogans.

    Science.gov (United States)

    He, Ping; Deng, Cong; Liu, Boyu; Zeng, LingBing; Zhao, Wei; Zhang, Yan; Jiang, XuCheng; Guo, XiaoKui; Qin, JinHong

    2013-11-01

    Alarmone Guanosine 5'-diphosphate (or 5'-triphosphate) 3'-diphosphate [(p)ppGpp] is the key component that globally regulates stringent control in bacteria. There are two homologous enzymes, RelA and SpoT in Escherichia coli, which are responsible for fluctuations in (p)ppGpp concentration inside the cell, whereas there exists only a single RelA/SpoT enzyme in Gram-positive bacteria. We have identified a bifunctional enzyme with (p)ppGpp-hydrolase/synthase activity in Leptospira interrogans. We show that the relLin gene (LA_3085) encodes a protein that fully complements the relA/spoT double mutants in E. coli. The protein functions as a (p)ppGpp degradase as well as a (p)ppGpp synthase when the cells encounter amino acid stress and deprivation of carbon sources. N-terminus HD and RSD domains of relLin (relLinN ) were observed to restore growth of double mutants of E. coli. Finally, We demonstrate that purified RelLin and RelLinN show high (p)ppGpp synthesis activity in vitro. Taken together, our results suggest that L. interrogans contain a single Rel-like bifunctional protein, RelLin , which plays an important role in maintaining the basal level of (p)ppGpp in the cell potentially contributing to the regulation of bacterial stress response.

  15. RCP is a human breast cancer-promoting gene with Ras-activating function.

    Science.gov (United States)

    Zhang, Jinqiu; Liu, Xuejing; Datta, Arpita; Govindarajan, Kunde; Tam, Wai Leong; Han, Jianyong; George, Joshy; Wong, Christopher; Ramnarayanan, Kalpana; Phua, Tze Yoong; Leong, Wan Yee; Chan, Yang Sun; Palanisamy, Nallasivam; Liu, Edison Tak-Bun; Karuturi, Krishna Murthy; Lim, Bing; Miller, Lance David

    2009-08-01

    Aggressive forms of cancer are often defined by recurrent chromosomal alterations, yet in most cases, the causal or contributing genetic components remain poorly understood. Here, we utilized microarray informatics to identify candidate oncogenes potentially contributing to aggressive breast cancer behavior. We identified the Rab-coupling protein RCP (also known as RAB11FIP1), which is located at a chromosomal region frequently amplified in breast cancer (8p11-12) as a potential candidate. Overexpression of RCP in MCF10A normal human mammary epithelial cells resulted in acquisition of tumorigenic properties such as loss of contact inhibition, growth-factor independence, and anchorage-independent growth. Conversely, knockdown of RCP in human breast cancer cell lines inhibited colony formation, invasion, and migration in vitro and markedly reduced tumor formation and metastasis in mouse xenograft models. Overexpression of RCP enhanced ERK phosphorylation and increased Ras activation in vitro. As these results indicate that RCP is a multifunctional gene frequently amplified in breast cancer that encodes a protein with Ras-activating function, we suggest it has potential importance as a therapeutic target. Furthermore, these studies provide new insight into the emerging role of the Rab family of small G proteins and their interacting partners in carcinogenesis.

  16. RCP is a human breast cancer–promoting gene with Ras-activating function

    Science.gov (United States)

    Zhang, Jinqiu; Liu, Xuejing; Datta, Arpita; Govindarajan, Kunde; Tam, Wai Leong; Han, Jianyong; George, Joshy; Wong, Christopher; Ramnarayanan, Kalpana; Phua, Tze Yoong; Leong, Wan Yee; Chan, Yang Sun; Palanisamy, Nallasivam; Liu, Edison Tak-Bun; Karuturi, Krishna Murthy; Lim, Bing; Miller, Lance David

    2009-01-01

    Aggressive forms of cancer are often defined by recurrent chromosomal alterations, yet in most cases, the causal or contributing genetic components remain poorly understood. Here, we utilized microarray informatics to identify candidate oncogenes potentially contributing to aggressive breast cancer behavior. We identified the Rab-coupling protein RCP (also known as RAB11FIP1), which is located at a chromosomal region frequently amplified in breast cancer (8p11–12) as a potential candidate. Overexpression of RCP in MCF10A normal human mammary epithelial cells resulted in acquisition of tumorigenic properties such as loss of contact inhibition, growth-factor independence, and anchorage-independent growth. Conversely, knockdown of RCP in human breast cancer cell lines inhibited colony formation, invasion, and migration in vitro and markedly reduced tumor formation and metastasis in mouse xenograft models. Overexpression of RCP enhanced ERK phosphorylation and increased Ras activation in vitro. As these results indicate that RCP is a multifunctional gene frequently amplified in breast cancer that encodes a protein with Ras-activating function, we suggest it has potential importance as a therapeutic target. Furthermore, these studies provide new insight into the emerging role of the Rab family of small G proteins and their interacting partners in carcinogenesis. PMID:19620787

  17. Domestic activity for technical development of the APR1400's RCP performance test

    Energy Technology Data Exchange (ETDEWEB)

    Cho, Seok; Kim, Seok; Bae, Byung-Uhn; Cho, Yun-Jae; Kim, Yeon-Sik; Jeon, Woo-Jin; Yun, Young-Jung [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2015-10-15

    The thermal hydraulic and electric capability of the RCP test facility (RCPTF) covers up to 18.5 MPa, 343 .deg. C, 11.7 m{sup 3}/s, and 14.0 MW in the design pressure, temperature, flow rate, and the maximum electric power, respectively. In 2013, commissioning test had been performed to verify its designed capability, followed by several modifications in the RCPTF including signal processing and control logic to enhance verification and evaluation capability of the RCP performance. After finishing the commissioning and modification of the RCPTF, type test for the new-type RCP had been performed successfully. In this paper, several technical issues developed in the 2013 and the type test's method and results will be described. In the present paper, the technical activities for the development of the verification test of APR1400's RCP are described. KAERI has completed the full set of technology development, prerequisite for the RCP verification test, and now on the way to perform a test for the sealing capacity of the seal assembly during the Station Block Out (SBO) condition of APR1400.

  18. Generation of mammalian host-adapted Leptospira interrogans by cultivation in peritoneal dialysis membrane chamber implantation in rats

    Science.gov (United States)

    Leptospira interrogans can infect a myriad of mammalian hosts, including humans (Bharti, Nally et al. 2003, Ko, Goarant et al. 2009). Following acquisition by a suitable host, leptospires disseminate via the bloodstream to multiple tissues, including the kidneys, where they adhere to and colonize th...

  19. Leptospira Interrogans induces fibrosis in the mouse kidney through Inos-dependent, TLR- and NLR-independent signaling pathways.

    Directory of Open Access Journals (Sweden)

    Martine Fanton d'Andon

    Full Text Available BACKGROUND: Leptospira (L. interrogans are bacteria responsible for a worldwide reemerging zoonosis. Rodents carry L. interrogans asymptomatically in their kidneys and excrete bacteria in the urine, contaminating the environment. Humans get infected through skin contact and develop a mild or severe leptospirosis that may lead to renal failure and fibrosis. L. interrogans provoke an interstitial nephritis, but the induction of fibrosis caused by L. interrogans has not been studied in murine models. Innate immune receptors from the TLR and NLR families have recently been shown to play a role in the development and progression of tissue fibrosis in the lung, liver and kidneys under different pathophysiological situations. We recently showed that TLR2, TLR4, and NLRP3 receptors were crucial in the defense against leptospirosis. Moreover, infection of a human cell line with L. interrogans was shown to induce TLR2-dependent production of fibronectin, a component of the extracellular matrix. Therefore, we thought to assess the presence of renal fibrosis in L. interrogans infected mice and to analyze the contribution of some innate immune pathways in this process. METHODOLOGY/PRINCIPAL FINDINGS: Here, we characterized by immunohistochemical studies and quantitative real-time PCR, a model of Leptospira-infected C57BL/6J mice, with chronic carriage of L. interrogans inducing mild renal fibrosis. Using various strains of transgenic mice, we determined that the renal infiltrates of T cells and, unexpectedly, TLR and NLR receptors, are not required to generate Leptospira-induced renal fibrosis. We also show that the iNOS enzyme, known to play a role in Leptospira-induced interstitial nephritis, also plays a role in the induction of renal fibrosis. CONCLUSION/SIGNIFICANCE: To our knowledge, this work provides the first experimental murine model of sustained renal fibrosis induced by a chronic bacterial infection that may be peculiar, since it does not rely on

  20. Implications of RCP emissions on future PM2.5 air quality and direct radiative forcing over China

    Science.gov (United States)

    Li, Ke; Liao, Hong; Zhu, Jia; Moch, Jonathan M.

    2016-11-01

    Severe PM2.5 air pollution in China and the First Grand National Standard (FGNS), implemented in 2016 (annual PM2.5 concentration target of less than 35 µg m-3), necessitate urgent reduction strategies. This study applied the nested-grid version of the Goddard Earth Observing System (GEOS) chemical transport model (GEOS-Chem) to quantify 2000-2050 changes in PM2.5 air quality and related direct radiative forcing (DRF) in China, based on future emission changes under the representative concentration pathway (RCP) scenarios of RCP2.6, RCP4.5, RCP6.0, and RCP8.5. In the near term (2000-2030), a projected maximum increase in PM2.5 concentrations of 10-15 µg m-3 is found over east China under RCP6.0 and RCP8.5 and less than 5 µg m-3 under RCP2.6 and RCP4.5. In the long term (2000-2050), PM2.5 pollution clearly improves, and the largest decrease in PM2.5 concentrations of 15-30 µg m-3 is over east China under all RCPs except RCP6.0. Focusing particularly on highly polluted regions, we find that Beijing-Tianjin-Hebei (BTH) wintertime PM2.5 concentrations meeting the FGNS occur after 2040 under RCP2.6, RCP4.5, and RCP8.5, and summertime PM2.5 concentrations reach this goal by 2030 under RCP2.6 and RCP4.5. In Sichuan Basin (SCB), wintertime PM2.5 concentrations below the FGNS occur only in 2050 under RCP2.6 and RCP4.5, although future summertime PM2.5 will be well controlled. The difficulty in controlling future PM2.5 concentrations relates to unmitigated high levels of nitrate, although NOx and SO2 emissions show substantial reductions during 2020-2040. The changes in aerosol concentrations lead to positive aerosol DRF over east China (20°-45°N, 100°-125°E) by 1.22, 1.88, and 0.66 W m-2 in 2050 relative to 2000 under RCP2.6, RCP4.5, and RCP8.5, respectively. When considering both health and climate effects of PM2.5 over China, for example, PM2.5 concentrations averaged over east China under RCP4.5 (RCP2.6) decrease by 54% (43%) in 2050 relative to 2000, but at the

  1. Efecto booster de una inmunización activa con Leptospira interrogans serogrupo Ballum en hámsters vacunados con vax-SPIRAL®

    Directory of Open Access Journals (Sweden)

    Andrés González

    2003-06-01

    Full Text Available Se evaluó en hámsters la inmunidad cruzada y capacidad protectora conferidas por la vacuna antileptospirósica trivalente vax-SPIRAL® frente a Leptospira interrogans serogrupo Ballum, así como el efecto de una inmunización activa con preparación vacunal monovalente de Ballum en animales previamente inmunizados con la vacuna trivalente. La respuesta IgG específica a Ballum, Canicola, Icterohaemorrhagiae y Pomona inducida tras cada inmunización se determinó mediante un sistema ELISA cuantitativo de células enteras. La capacidad de protección heteróloga de vax-SPIRAL® se evaluó frente al reto con 100 y 10 000 DL50 de dos cepas clínicas de Ballum altamente virulentas. Tras 14 días de culminado el esquema de inmunización la vacuna trivalente indujo un 100% de seroconversión de IgG específica a Ballum en los animales inmunizados y una total protección cruzada frente a la infección letal y la prevalencia de leptospiras en hígado y riñón. La aplicación de una única dosis de preparación monovalente de Ballum tras 6 semanas de culminado el esquema de inmunización de vax-SPIRAL® reforzó la inmunidad frente a los cuatro serogrupos de Leptospira de mayor circulación en humanos en Cuba.

  2. [Assessment of cancer RCP meetings in Rhône-Alpes: a survey on the ground].

    Science.gov (United States)

    Descotes, J-L; Guillem, P; Bondil, P; Colombel, M; Chabloz, C

    2010-10-01

    The results of a local survey sent to urologists, oncologists and radiotherapeutists working in Rhône-Alpes have been reported to assess the value of multidisciplinary oncological meetings (RCP) in Urology. The results of this short study have been analyzed and compared to the national results published by the Inspection Générale des Affaires Sociales report. Meanwhile, we have created a professional electronic directory collecting all RCP of Rhône-Alpes, which will be accessible soon. Copyright © 2010. Published by Elsevier Masson SAS.

  3. Rab coupling protein (RCP): a novel target of progesterone action in primate endometrium.

    Science.gov (United States)

    Patil, V S; Sachdeva, G; Modi, D N; Katkam, R R; Manjramkar, D D; Hinduja, I; Puri, C P

    2005-10-01

    Acquisition of functional receptivity by the endometrium is assumed to be effected by progesterone-dependent expression and repression of several genes during the implantation window in a menstrual cycle. In the present study, we employed differential display (DD) reverse transcription-polymerase chain reaction (RT-PCR) to identify progesterone-dependent gene/gene fragments that are differentially expressed during the peri-implantation phase in receptive and nonreceptive endometria, obtained from fertile and infertile bonnet monkeys respectively. Receptive endometria were obtained from regularly cycling (n=5) fertile female bonnet monkeys. Endometrial nonreceptivity was induced by treating bonnet monkeys with either 2.5 mg (n=5) or 5.0 mg (n=5) onapristone (ZK 98.299), an antiprogestin, on every third day for one cycle. Ovulation, levels of circulatory hormones (estradiol and progesterone) and menstrual cycle length did not change in treated animals; however, endometrial growth was retarded. DD2, one of the differentially expressed cDNA fragments, showed higher representation in nonreceptive endometria than in receptive endometria. The DD2 sequence was found to be homologous to the sequence of the carboxyl terminal region of Rab coupling protein (RCP), a recently discovered protein involved in intracellular vesicular trafficking. To confirm the identity of DD2 as RCP, RT-PCR studies were carried out with a forward primer deduced from the RCP sequence and a reverse primer from the DD2 sequence. The product (DDRCP) obtained, when sequenced, revealed 95% homology with the nucleotide number 1196-1757 of human RCP cDNA. Furthermore, the pattern of DDRCP expression at transcript level was found to be similar to that shown by DD2; that is, it was higher in nonreceptive endometrium. Northern analysis using labeled DD2 or DDRCP cDNA fragments identified two transcripts of 6.0 and 4.0 kb in human endometrium. In situ hybridization studies using digoxigenin-labeled DD2

  4. Prevalence of antibodies against Borrelia burgdorferi, Anaplasma phagocytophilum, and Leptospira interrogans serovars in Bernese Mountain Dogs.

    Science.gov (United States)

    Preyß-Jägeler, C; Müller, E; Straubinger, R K; Hartmann, K

    2016-01-01

    Bernese Mountain Dogs (BMD) have a higher prevalence of Borrelia burgdorferi sensu lato (Bbsl) antibodies than other breeds, but it is not known whether this is the case for other pathogens. Therefore, the aim of the study was to determine the frequency and level of specific antibodies against members of the Bbsl group, Anaplasma phagocytophilum (Ap), and Leptospira (L.) interrogans serovars in BMD and compare the results with those found in dogs of other breeds. A total of 171 healthy BMD and 57 healthy control dogs of other breeds were included in the study. Controls were large dogs (> 30 kg) with long, dark hair coats. A two-tiered testing method consisting of computerized kinetic enzyme-linked immunosorbent assay (KELA) and Western blotting was used for detection of antibodies against Bbsl, an immunofluorescence assay (IFA) was used for detection of antibodies against Ap, and microscopic agglutination test (MAT) for antibodies to 18 different serovars of L. interrogans. The prevalence of anti-Bbsl antibodies was significantly higher in BMD (43.3%) than in controls (17.5%) (p < 0.001). Antibodies to Bbsl attributable to vaccination were excluded from the calculation of prevalence. Antibodies to Ap were found in 50.3% of BMD, whereas only 24.6% of the controls dogs were tested positive for Ap (p < 0.001). Antibody titers of the 18 different serovars of L. interrogans antibodies did not differ significantly between BMD and control dogs except for L. copenhageni antibody titers which were higher in BMD. Significantly higher antibody titers to L. canicola (p = 0.003), L. copenhageni (p = 0.005), L. grippothyphosa (p = 0.029) and L. vanderhoedoni (p = 0.035) were seen in BMD compared to control dogs. BMD had a higher prevalence of anti-Bbsl, anti-L. copenhageni and anti-Ap antibodies than control dogs. Significantly higher antibody titers against L. canicola (p = 0.003), L. copenhageni (p = 0.005), L. grippothyphosa (p = 0.029) and L

  5. Overexpression and Purification of Human Calcitonin Gene Related Peptide - Receptor Component Protein (CGRP-RCP) in Escherichia coli

    Science.gov (United States)

    Tolun, Adviye A.; Dickerson, Ian M.; Malhotra, Arun

    2007-01-01

    Calcitonin gene-related peptide (CGRP) is a neuropeptide secreted by the central and peripheral nervous system nerves that has important physiological functions such as vasodilation, cardiotonic actions, metabolic and pro-inflammatory effects. The CGRP receptor is unique among G-protein coupled receptors in that a functional CGRP receptor consists of at least three proteins: Calcitonin Like Receptor (CLR), Receptor Activity Modifying Protein (RAMP1) and Receptor Component Protein (RCP). RCP is a required factor in CGRP-mediated signal transduction and it couples the CGRP receptor to the signal transduction pathway. Here we describe methods to overexpress and purify RCP for structure-function studies. Human RCP was cloned and overexpressed with a poly-histidine tag and as a Maltose Binding Protein (MBP) fusion in Escherichia coli using commercially available expression vectors. While his-tagged RCP is prone to aggregation, solubility is improved when RCP is expressed as a MBP fusion. Expression and purification procedures for these constructs are described. Results from these studies will facilitate structural analysis of human RCP, and allow further understanding of RCP function. PMID:17067815

  6. Leptospira interrogans at the human-wildlife interface in northern Botswana: a newly identified public health threat.

    Science.gov (United States)

    Jobbins, S E; Sanderson, C E; Alexander, K A

    2014-03-01

    Leptospirosis is the most widespread zoonosis in the world. In northern Botswana, humans live in close proximity to a diversity of wildlife and peridomestic rodents and may be exposed to a variety of zoonotic pathogens. Little is known regarding the occurrence and epidemiology of L. interrogans in Africa despite the recognized global importance of this zoonotic disease and the threat it poses to public health. In Botswana, banded mongooses (Mungos mungo) live in close proximity to humans across protected and unprotected landscapes and may be a useful sentinel species for assessing the occurrence of zoonotic organisms, such as L. interrogans. We utilized PCR to screen banded mongoose kidneys for leptospiral DNA and identified 41.5% prevalence of renal carriage of L. interrogans (exact binomial 95% CI 27.7-56.7%, n = 41). Renal carriage was also detected in one Selous' mongoose (Paracynictis selousi). This is the first published confirmation of carriage of L. interrogans in either species. This is also the first report of L. interrogans occurrence in northern Botswana and the only report of this organism in a wildlife host in the country. Pathogenic Leptospira are usually transmitted indirectly to humans through soil or water contaminated with infected urine. Other avenues, such as direct contact between humans and wildlife, as well as consumption of mongooses and other wildlife as bushmeat, may pose additional exposure risk and must be considered in public health management of this newly identified zoonotic disease threat. There is a critical need to characterize host species involvement and pathogen transmission dynamics, including human-wildlife interactions that may increase human exposure potential and infection risk. We recommend that public health strategy be modified to include sensitization of medical practitioners to the presence of L. interrogans in the region, the potential for human infection, and implementation of clinical screening. This study

  7. Rab coupling protein (RCP), a novel Rab4 and Rab11 effector protein.

    Science.gov (United States)

    Lindsay, Andrew J; Hendrick, Alan G; Cantalupo, Giuseppina; Senic-Matuglia, Francesca; Goud, Bruno; Bucci, Cecilia; McCaffrey, Mary W

    2002-04-05

    Rab4 and Rab11 are small GTPases belonging to the Ras superfamily. They both function as regulators along the receptor recycling pathway. We have identified a novel 80-kDa protein that interacts specifically with the GTP-bound conformation of Rab4, and subsequent work has shown that it also interacts strongly with Rab11. We name this protein Rab coupling protein (RCP). RCP is predominantly membrane-bound and is expressed in all cell lines and tissues tested. It colocalizes with early endosomal markers including Rab4 and Rab11 as well as with the transferrin receptor. Overexpression of the carboxyl-terminal region of RCP, which contains the Rab4- and Rab11-interacting domain, results in a dramatic tubulation of the transferrin compartment. Furthermore, expression of this mutant causes a significant reduction in endosomal recycling without affecting ligand uptake or degradation in quantitative assays. RCP is a homologue of Rip11 and therefore belongs to the recently described Rab11-FIP family.

  8. Immunocontraceptive potential of recombinantly expressed minimized chicken riboflavin carrier protein (mini-RCP) in rodents.

    Science.gov (United States)

    Subramanian, Sarada; Karandeb, Anjali A; Adiga, P Radhakantha

    2004-12-01

    Chicken riboflavin carrier protein (RCP; 219 AA) harbours four linear epitopes, constituted by the peptide residues 3-23, 64-83, 130-147 and 200-219. Antibodies to these sequences bioneutralize maternal RCP and provide protection from pregnancy in rodents. In order to overcome the major histocompatibility complex-dependent variability in immune response often encountered with use of single peptides for vaccination in genetically outbred populations, we have assembled a novel synthetic gene, incorporating in tandem the nucleotide sequences coding for all the four neutralizing epitopes of chicken RCP and expressed in Escherichia coli. The gene product, mini-RCP has been characterized for its immunogenic properties and contraceptive potential in rodents. Immunization of rabbits and rats led to generation of antibodies against individual peptide components, as determined by enzyme-linked-immunosorbent assay (ELISA). However, immunized rats carried pregnancy to term and delivered healthy offsprings. Antisera from these rats exhibited decreased affinity of binding to the native protein. These findings suggest that the prospects of covalently-linked epitope peptides need to be cautiously evaluated during the design and development of peptide-based vaccines.

  9. Leptospira interrogans serovar hardjo vaccination of pregnant beef cows and subsequent growth rate of progeny.

    Science.gov (United States)

    Holroyd, R G

    1980-10-01

    Five experiments with Leptospira interrogans serovar hardjo vaccine were carried out over a 6-year period in pregnant Brahman-cross and Sahiwal-cross cows in the dry tropics on northern Queensland. The numbers ranged from 127 breeders aged 2 to 5 years in 1972 to 344 breeders aged 2 to 9 years in 1977. Half of the cows were vaccinated twice in mid-pregnancy except for 1977, when they were vaccinated once. In 1975-1977 inclusive, half of the heifers were given an additional dose of vaccine at commencement of mating. Vaccination caused a significant (P less than 0.01) reduction of prenatal loss but not of perinatal or postnatal losses. Growth rates of calves from vaccinated and unvaccinated dams were similar.

  10. Production and characterization of monoclonal antibodies to the edta extract of Leptospira interrogans, serovar icterohaemorrhagiae

    Directory of Open Access Journals (Sweden)

    Lilian Terezinha de Queiroz Leite

    1996-10-01

    Full Text Available Monoclonal antibodies (MABs ivere produced against an etbylenediaminetetraacetate (EDTA extract of Leptospira interrogans serovar icterohaemorrhagiae being characterized by gel precipitation as IgM and IgG (IgGl and IgG2b. The EDTA extract was detected as several bands by silver staining in SDS-PAGE. In the Western blot the bands around 20 KDa reacted with a monoclonal antibody, 47B4D6, and was oxidized by periodate and was not digested by pronase, suggesting that the determinant is of carbohydrate nature, lmmunocytochemistry, using colloidal gold labeling, showed that an EDTA extract determinant recognized by monoclonal antibody 47B4D6, is localized under the outer envelope of serovar icterohaemorrhagiae. Hoe AIAB raised against the EDTA extract was not able to protect hamsters from lethal challenge with virulent homologous leptospires.

  11. Experimental infection of calves and sheep with Leptospira interrogans serovar balcanica.

    Science.gov (United States)

    Durfee, P T; Presidente, P J

    1979-10-01

    Two of four calves inoculated with Leptospira interrogans serovar balcanica developed low microscopic agglutinating (MA) titres to serovar hardjo. A third calf had an MA titre of 1:1024 by day 19 post-inoculation (PI). Transient leptospiruria was recorded in one calf on days 12 and 13 PI. An in-contact calf did not seroconvert. None of the calves had fever or other clinical signs of disease. Four ewes inoculated with balcanica developed MA titres to hardjo by day 13 PI, and a transient leptospiruria between days 14 and 25 PI. None of the ewes showed any evidence of clinical disease and three of them delivered healthy lambs 22 to 64 days PI. One ewe had mild lesions of focal interstitial nephritis.

  12. Kinetics of Leptospira interrogans infection in hamsters after intradermal and subcutaneous challenge.

    Directory of Open Access Journals (Sweden)

    Mariana L Coutinho

    2014-11-01

    Full Text Available Leptospirosis is a zoonosis caused by highly motile, helically shaped bacteria that penetrate the skin and mucous membranes through lesions or abrasions, and rapidly disseminate throughout the body. Although the intraperitoneal route of infection is widely used to experimentally inoculate hamsters, this challenge route does not represent a natural route of infection.Here we describe the kinetics of disease and infection in hamster model of leptospirosis after subcutaneous and intradermal inoculation of Leptospira interrogans serovar Copenhageni, strain Fiocruz L1-130. Histopathologic changes in and around the kidney, including glomerular and tubular damage and interstitial inflammatory changes, began on day 5, and preceded deterioration in renal function as measured by serum creatinine. Weight loss, hemoconcentration, increased absolute neutrophil counts (ANC in the blood and hepatic dysfunction were first noted on day 6. Vascular endothelial growth factor, a serum marker of sepsis severity, became elevated during the later stages of infection. The burden of infection, as measured by quantitative PCR, was highest in the kidney and peaked on day 5 after intradermal challenge and on day 6 after subcutaneous challenge. Compared to subcutaneous challenge, intradermal challenge resulted in a lower burden of infection in both the kidney and liver on day 6, lower ANC and less weight loss on day 7.The intradermal and subcutaneous challenge routes result in significant differences in the kinetics of dissemination and disease after challenge with L. interrogans serovar Copenhageni strain Fiocruz L1-130 at an experimental dose of 2×106 leptospires. These results provide new information regarding infection kinetics in the hamster model of leptospirosis.

  13. Production and characterization of monoclonal antibodies to the edta extract of Leptospira interrogans, serovar icterohaemorrhagiae

    Directory of Open Access Journals (Sweden)

    Lilian Terezinha de Queiroz Leite

    1996-10-01

    Full Text Available Monoclonal antibodies (MABs ivere produced against an etbylenediaminetetraacetate (EDTA extract of Leptospira interrogans serovar icterohaemorrhagiae being characterized by gel precipitation as IgM and IgG (IgGl and IgG2b. The EDTA extract was detected as several bands by silver staining in SDS-PAGE. In the Western blot the bands around 20 KDa reacted with a monoclonal antibody, 47B4D6, and was oxidized by periodate and was not digested by pronase, suggesting that the determinant is of carbohydrate nature, lmmunocytochemistry, using colloidal gold labeling, showed that an EDTA extract determinant recognized by monoclonal antibody 47B4D6, is localized under the outer envelope of serovar icterohaemorrhagiae. Hoe AIAB raised against the EDTA extract was not able to protect hamsters from lethal challenge with virulent homologous leptospires.Anticorpos monoclonais (AcM foram produzidos contra o extrato EDTA obtido de Leptospira interrogans, sorovar icterohaemorrhagiae. Pelo teste de precipitação foram caracterizados como IgM e IgG (IgGl e IgG2. A eletroforese em gel de poliacrilamida do extrato EDTA revelou diversas bandas quando corada pela prata. No "Western blot", as bandas em torno de 20 kDa reagiram com o AcM 47B4D6, foram oxidadas pelo periodato e não digeridas pela pronase, sugerindo que o determinante é de natureza carboidrato. O determinante reconhecido pelo AcM 47B4D6 estã localizado sob o envelope externo como revelado pela imunocitoquímica usando marcação com ouro coloidal. O AcM contra extrato EDTA do sorovar icterohaemorrahagiae não protegeu hamsters quando inoculados com lepstopira homóloga virulenta.

  14. Global transcriptomic response of Leptospira interrogans serovar Copenhageni upon exposure to serum

    Directory of Open Access Journals (Sweden)

    Lo Miranda

    2010-01-01

    Full Text Available Abstract Background Leptospirosis is a zoonosis of worldwide distribution caused by infection with pathogenic serovars of Leptospira spp. The most common species, L. interrogans, can survive in the environment for lengthy periods of time in between infection of mammalian hosts. Transmission of pathogenic Leptospira to humans mostly occurs through abraded skin or mucosal surfaces after direct or indirect contact with infected animals or contaminated soil or water. The spirochete then spreads hematogenously, resulting in multi-organ failure and death in severe cases. Previous DNA microarray studies have identified differentially expressed genes required for adaptation to temperature and osmolarity conditions inside the host compared to those of the environment. Results In order to identify genes involved in survival in the early spirochetemic phase of infection, we performed a transcriptional analysis of L. interrogans serovar Copenhageni upon exposure to serum in comparison with EMJH medium. One hundred and sixty-eight genes were found to be differentially expressed, of which 55 were up-regulated and 113 were down-regulated. Genes of known or predicted function accounted for 54.5 and 45.1% of up- and down-regulated genes, respectively. Most of the differentially expressed genes were predicted to be involved in transcriptional regulation, translational process, two-component signal transduction systems, cell or membrane biogenesis, and metabolic pathways. Conclusions Our study showed global transcriptional changes of pathogenic Leptospira upon exposure to serum, representing a specific host environmental cue present in the bloodstream. The presence of serum led to a distinct pattern of gene expression in comparison to those of previous single-stimulus microarray studies on the effect of temperature and osmolarity upshift. The results provide insights into the pathogenesis of leptospirosis during the early bacteremic phase of infection.

  15. Adhesins of Leptospira interrogans Mediate the Interaction to Fibrinogen and Inhibit Fibrin Clot Formation In Vitro

    Science.gov (United States)

    Oliveira, Rosane; Domingos, Renan F.; Siqueira, Gabriela H.; Fernandes, Luis G.; Souza, Natalie M.; Vieira, Monica L.; de Morais, Zenaide M.; Vasconcellos, Silvio A.; Nascimento, Ana L. T. O.

    2013-01-01

    We report in this work that Leptospira strains, virulent L. interrogans serovar Copenhageni, attenuated L. interrogans serovar Copenhageni and saprophytic L. biflexa serovar Patoc are capable of binding fibrinogen (Fg). The interaction of leptospires with Fg inhibits thrombin- induced fibrin clot formation that may affect the haemostatic equilibrium. Additionally, we show that plasminogen (PLG)/plasmin (PLA) generation on the surface of Leptospira causes degradation of human Fg. The data suggest that PLA-coated leptospires were capable to employ their proteolytic activity to decrease one substrate of the coagulation cascade. We also present six leptospiral adhesins and PLG- interacting proteins, rLIC12238, Lsa33, Lsa30, OmpL1, rLIC11360 and rLIC11975, as novel Fg-binding proteins. The recombinant proteins interact with Fg in a dose-dependent and saturable fashion when increasing protein concentration was set to react to a fix human Fg concentration. The calculated dissociation equilibrium constants (KD) of these reactions ranged from 733.3±276.8 to 128±89.9 nM for rLIC12238 and Lsa33, respectively. The interaction of recombinant proteins with human Fg resulted in inhibition of fibrin clot by thrombin-catalyzed reaction, suggesting that these versatile proteins could mediate Fg interaction in Leptospira. Our data reveal for the first time the inhibition of fibrin clot by Leptospira spp. and presents adhesins that could mediate these interactions. Decreasing fibrin clot would cause an imbalance of the coagulation cascade that may facilitate bleeding and help bacteria dissemination PMID:24009788

  16. Receptor component protein (RCP): a member of a multi-protein complex required for G-protein-coupled signal transduction.

    Science.gov (United States)

    Prado, M A; Evans-Bain, B; Dickerson, I M

    2002-08-01

    The calcitonin-gene-related peptide (CGRP) receptor component protein (RCP) is a 148-amino-acid intracellular protein that is required for G-protein-coupled signal transduction at receptors for the neuropeptide CGRP. RCP works in conjunction with two other proteins to constitute a functional CGRP receptor: calcitonin-receptor-like receptor (CRLR) and receptor-activity-modifying protein 1 (RAMP1). CRLR has the stereotypical seven-transmembrane topology of a G-protein-coupled receptor; it requires RAMP1 for trafficking to the cell surface and for ligand specificity, and requires RCP for coupling to the cellular signal transduction pathway. We have made cell lines that expressed an antisense construct of RCP and determined that CGRP-mediated signal transduction was reduced, while CGRP binding was unaffected. Furthermore, signalling at two other endogenous G-protein-coupled receptors was unaffected, suggesting that RCP was specific for a limited subset of receptors.

  17. Future changes in precipitation of the baiu season under RCP scenarios

    Science.gov (United States)

    Okada, Y.; Takemi, T.; Ishikawa, H.

    2014-12-01

    Recently, the relationship between global warming and rainfall during the rainy season, which called the baiu in Japan, has been attracting attention in association with heavy rainfall in this period. In the Innovative Program of Climate Change Projection for the 21st Century, many studies show a delay in the northward march of the baiu front, and significant increase of daily precipitation amounts around western Japan during the late baiu season (e.g., Kusunoki et al. 2011, Kanada et al. 2012). The future climate experiment in these studies was performed under the IPCC SRES A1B scenarios for global warming conditions. In this study, we discuss the future changes in precipitation using calculated 60km-mesh model (MRI-AGCM3.2H) under Representative Concentration Pathways (RCP) scenarios. Support of this dataset is provided by the Meteorological Research Institute (MRI). These dataset are calculated by setting the Yoshimura (YS) scheme mainly.Seasonal progression of future precipitation generally indicates the northward in RCP2.6 and 4.5 scenarios, around western Japan. In RCP6.0 scenario, precipitation intensity is weak compared to the other scenarios. RCP8.5 scenario is calculated by setting three different cumulus schemes (YS, Arakawa-Schubert (AS), and Kain-Fritsch (KF) schemes). RCP8.5 configured in YS scheme showed that the rainband associated with the baiu front is not clear. Moreover, peak is remarkable during late June. In AS scheme, the precipitation area stagnates around 30 N until August. And it in KF scheme shows gradual northward migration.This work was conducted under the Program for Risk Information on Climate Change supported by the Ministry of Education, Culture, Sports, Science, and Technology-Japan (MEXT).

  18. Direct interactions between calcitonin-like receptor (CLR) and CGRP-receptor component protein (RCP) regulate CGRP receptor signaling.

    Science.gov (United States)

    Egea, Sophie C; Dickerson, Ian M

    2012-04-01

    Calcitonin gene-related peptide (CGRP) is a neuropeptide with multiple neuroendocrine roles, including vasodilation, migraine, and pain. The receptor for CGRP is a G protein-coupled receptor (GPCR) that requires three proteins for function. CGRP binds to a heterodimer composed of the GPCR calcitonin-like receptor (CLR) and receptor activity-modifying protein (RAMP1), a single transmembrane protein required for pharmacological specificity and trafficking of the CLR/RAMP1 complex to the cell surface. In addition, the CLR/RAMP1 complex requires a third protein named CGRP-receptor component protein (RCP) for signaling. Previous studies have demonstrated that depletion of RCP from cells inhibits CLR signaling, and in vivo studies have demonstrated that expression of RCP correlates with CLR signaling and CGRP efficacy. It is not known whether RCP interacts directly with CLR to exert its effect. The current studies identified a direct interaction between RCP and an intracellular domain of CLR using yeast two-hybrid analysis and coimmunoprecipitation. When this interacting domain of CLR was expressed as a soluble fusion protein, it coimmunoprecipitated with RCP and inhibited signaling from endogenous CLR. Expression of this dominant-negative domain of CLR did not significantly inhibit trafficking of CLR to the cell surface, and thus RCP may not have a chaperone function for CLR. Instead, RCP may regulate CLR signaling in the cell membrane, and direct interaction between RCP and CLR is required for CLR activation. To date, RCP has been found to interact only with CLR and represents a novel neuroendocrine regulatory step in GPCR signaling.

  19. Dual nuclease activity of a Cas2 protein in CRISPR-Cas subtype I-B of Leptospira interrogans.

    Science.gov (United States)

    Dixit, Bhuvan; Ghosh, Karukriti Kaushik; Fernandes, Gary; Kumar, Pankaj; Gogoi, Prerana; Kumar, Manish

    2016-04-01

    Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130 carries a set of cas genes associated with CRISPR-Cas subtype I-B. Herein, we report for the first time active transcription of a set of cas genes (cas1 to cas8) of L. interrogans where cas4, cas1, cas2 and cas6, cas3, cas8, cas7, cas5 are clustered together in two independent operons. As an initial step toward comprehensive understanding of CRISPR-Cas system in spirochete, the biochemical study of one of the core Leptospira Cas2 proteins (Lep_Cas2) showed nuclease activity on both DNA and RNA in a nonspecific manner. Additionally, unlike other known Cas2 proteins, Lep_Cas2 showed metal-independent RNase activity and preferential activity on RNA over DNA. These results provide insight for understanding Cas2 diversity existing in the prokaryotic adaptive immune system.

  20. Proteína LIC10494 de Leptospira interrogans serovar Copenhageni: modelo estructural y regiones funcionales asociadas

    OpenAIRE

    Orlando Emilio Acevedo; George Emílio Barreto; Janneth González-Santos

    2012-01-01

    Protein LIC10494 of Leptospira interrogans serovar Copenhageni: structural model and associated functional regions. Objective.Predict by computational means the 3D structure of the antigenic protein LIC10494 and report associated important functional regionsfor its pathogenicity and immunogenicity. Materials and methods. We performed a computational analysis of the primary structure ofLIC10494 using the servers BLAST, PROTPARAM, PROTSCALE, DAS, SOSUI, TOPPRED, TMAP, TMpred, SPLIT4, PHDHTM,TMH...

  1. Mathematizing Process of Junior High School Students to Improve Mathematics Literacy Refers PISA on RCP Learning

    Science.gov (United States)

    Wardono; Mariani, S.; Hendikawati, P.; Ikayani

    2017-04-01

    Mathematizing process (MP) is the process of modeling a phenomenon mathematically or establish the concept of a phenomenon. There are two mathematizing that is Mathematizing Horizontal (MH) and Mathematizing Vertical (MV). MH as events changes contextual problems into mathematical problems, while MV is the process of formulation of the problem into a variety of settlement mathematics by using some appropriate rules. Mathematics Literacy (ML) is the ability to formulate, implement and interpret mathematics in various contexts, including the capacity to perform reasoning mathematically and using the concepts, procedures, and facts to describe, explain or predict phenomena incident. If junior high school students are conditioned continuously to conduct mathematizing activities on RCP (RME-Card Problem) learning, it will be able to improve ML that refers PISA. The purpose of this research is to know the capability of the MP grade VIII on ML content shape and space with the matter of the cube and beams with RCP learning better than the scientific learning, upgrade MP grade VIII in the issue of the cube and beams with RCP learning better than the scientific learning in terms of cognitive styles reflective and impulsive the MP grade VIII with the approach of the RCP learning in terms of cognitive styles reflective and impulsive This research is the mixed methods model concurrent embedded. The population in this study, i.e., class VIII SMPN 1 Batang with sample two class. Data were taken with the observation, interviews, and tests and analyzed with a different test average of one party the right qualitative and descriptive. The results of this study demonstrate the capability of the MP student with RCP learning better than the scientific learning, upgrade MP with RCP learning better compare with scientific learning in term cognitive style of reflective and impulsive. The subject of the reflective group top, middle, and bottom can meet all the process of MH indicators are

  2. Generation of Mammalian Host-adapted Leptospira interrogans by Cultivation in Peritoneal Dialysis Membrane Chamber Implantation in Rats.

    Science.gov (United States)

    Grassmann, André Alex; McBride, Alan John Alexander; Nally, Jarlath E; Caimano, Melissa J

    2015-07-20

    Leptospira interrogans can infect a myriad of mammalian hosts, including humans (Bharti et al., 2003; Ko et al., 2009). Following acquisition by a suitable host, leptospires disseminate via the bloodstream to multiple tissues, including the kidneys, where they adhere to and colonize the proximal convoluted renal tubules (Athanazio et al., 2008). Infected hosts shed large number of spirochetes in their urine and the leptospires can survive in different environmental conditions before transmission to another host. Differential gene expression by Leptospira spp. permits adaption to these new conditions. Here we describe a protocol for the cultivation of Leptospira interrogans within Dialysis Membrane Chambers (DMCs) implanted into the peritoneal cavities of Sprague-Dawley rats (Caimano et al., 2014). This technique was originally developed to study mammalian adaption by the Lyme disease spirochete, Borrelia burgdorferi (Akins et al., 1998; Caimano, 2005). The small pore size (8,000 MWCO) of the dialysis membrane tubing used for this procedure permits access to host nutrients but excludes host antibodies and immune effector cells. Given the physiological and environmental similarities between DMCs and the proximal convoluted renal tubule, we reasoned that the DMC model would be suitable for studying in vivo gene expression by L. interrogans. In a 20 to 30 min procedure, DMCs containing virulent leptospires are surgically-implanted into the rat peritoneal cavity. Nine to 11 days post-implantation, DMCs are explanted and organisms recovered. Typically, a single DMC yields ~10(9) mammalian host-adapted leptospires (Caimano et al., 2014). In addition to providing a facile system for studying the transcriptional and physiologic changes pathogenic L. interrogans undergo within the mammal, the DMC model also provides a rationale basis for selecting new targets for mutagenesis and the identification of novel virulence determinants. Caution: Leptospira interrogans is a BSL-2

  3. 一种改进的RCP算法%Modified RCP Algorithm

    Institute of Scientific and Technical Information of China (English)

    陈栩杉; 武欣嵘; 周伟; 周斌; 吴凯

    2011-01-01

    RCP is a rate-based explicit congestion control algorithm.It performs well on the efficiency convergence and fairness convergence and has a good performance under the multiplebottleneck network topology environment.But some researches show that when a new flow joins in the network,there exists in RCP algorithm severe instantaneous overloading,resulting in packet loss.The source control was adjusted to the algorithm and S-RCP algorithm proposed. Theoretic analysis and simulation results confirm that S-RCP algorithm solves the instantaneous overloading to a certain extent and also eliminates the packet loss.%RCP算法是一种基于速率的显式拥塞控制算法,具有较好的效率和公平收敛性,在多瓶颈网络拓扑环境下也有很好的性能。但是研究表明,当一个新流加入网络时,RCP算法存在严重的瞬态过载现象,从而导致丢包的发生。文中通过在原有RCP算法基础上调整了源端控制的方式,提出了S-RCP算法。理论分析和仿真结果表明,S-RCP算法一定程度上解决了瞬态过载现象,基本消除了丢包现象。

  4. Post-translational modification of LipL32 during Leptospira interrogans infection.

    Directory of Open Access Journals (Sweden)

    Timothy D Witchell

    2014-10-01

    Full Text Available BACKGROUND: Leptospirosis, a re-emerging disease of global importance caused by pathogenic Leptospira spp., is considered the world's most widespread zoonotic disease. Rats serve as asymptomatic carriers of pathogenic Leptospira and are critical for disease spread. In such reservoir hosts, leptospires colonize the kidney, are shed in the urine, persist in fresh water and gain access to a new mammalian host through breaches in the skin. METHODOLOGY/PRINCIPAL FINDINGS: Previous studies have provided evidence for post-translational modification (PTM of leptospiral proteins. In the current study, we used proteomic analyses to determine the presence of PTMs on the highly abundant leptospiral protein, LipL32, from rat urine-isolated L. interrogans serovar Copenhageni compared to in vitro-grown organisms. We observed either acetylation or tri-methylation of lysine residues within multiple LipL32 peptides, including peptides corresponding to regions of LipL32 previously identified as epitopes. Intriguingly, the PTMs were unique to the LipL32 peptides originating from in vivo relative to in vitro grown leptospires. The identity of each modified lysine residue was confirmed by fragmentation pattern analysis of the peptide mass spectra. A synthetic peptide containing an identified tri-methylated lysine, which corresponds to a previously identified LipL32 epitope, demonstrated significantly reduced immunoreactivity with serum collected from leptospirosis patients compared to the peptide version lacking the tri-methylation. Further, a subset of the identified PTMs are in close proximity to the established calcium-binding and putative collagen-binding sites that have been identified within LipL32. CONCLUSIONS/SIGNIFICANCE: The exclusive detection of PTMs on lysine residues within LipL32 from in vivo-isolated L. interrogans implies that infection-generated modification of leptospiral proteins may have a biologically relevant function during the course of

  5. Predicting the responses of forest distribution and aboveground biomass to climate change under RCP scenarios in southern China.

    Science.gov (United States)

    Dai, Erfu; Wu, Zhuo; Ge, Quansheng; Xi, Weimin; Wang, Xiaofan

    2016-11-01

    In the past three decades, our global climate has been experiencing unprecedented warming. This warming has and will continue to significantly influence the structure and function of forest ecosystems. While studies have been conducted to explore the possible responses of forest landscapes to future climate change, the representative concentration pathways (RCPs) scenarios under the framework of the Coupled Model Intercomparison Project Phase 5 (CMIP5) have not been widely used in quantitative modeling research of forest landscapes. We used LANDIS-II, a forest dynamic landscape model, coupled with a forest ecosystem process model (PnET-II), to simulate spatial interactions and ecological succession processes under RCP scenarios, RCP2.6, RCP4.5 and RCP8.5, respectively. We also modeled a control scenario of extrapolating current climate conditions to examine changes in distribution and aboveground biomass (AGB) among five different forest types for the period of 2010-2100 in Taihe County in southern China, where subtropical coniferous plantations dominate. The results of the simulation show that climate change will significantly influence forest distribution and AGB. (i) Evergreen broad-leaved forests will expand into Chinese fir and Chinese weeping cypress forests. The area percentages of evergreen broad-leaved forests under RCP2.6, RCP4.5, RCP8.5 and the control scenarios account for 18.25%, 18.71%, 18.85% and 17.46% of total forest area, respectively. (ii) The total AGB under RCP4.5 will reach its highest level by the year 2100. Compared with the control scenarios, the total AGB under RCP2.6, RCP4.5 and RCP8.5 increases by 24.1%, 64.2% and 29.8%, respectively. (iii) The forest total AGB increases rapidly at first and then decreases slowly on the temporal dimension. (iv) Even though the fluctuation patterns of total AGB will remain consistent under various future climatic scenarios, there will be certain responsive differences among various forest types. © 2016

  6. CGRP-RCP, a novel protein required for signal transduction at calcitonin gene-related peptide and adrenomedullin receptors.

    Science.gov (United States)

    Evans, B N; Rosenblatt, M I; Mnayer, L O; Oliver, K R; Dickerson, I M

    2000-10-06

    It is becoming clear that receptors that initiate signal transduction by interacting with G-proteins do not function as monomers, but often require accessory proteins for function. Some of these accessory proteins are chaperones, required for correct transport of the receptor to the cell surface, but the function of many accessory proteins remains unknown. We determined the role of an accessory protein for the receptor for calcitonin gene-related peptide (CGRP), a potent vasodilator neuropeptide. We have previously shown that this accessory protein, the CGRP-receptor component protein (RCP), is expressed in CGRP responsive tissues and that RCP protein expression correlates with the biological efficacy of CGRP in vivo. However, the function of RCP has remained elusive. In this study stable cell lines were made that express antisense RCP RNA, and CGRP- and adrenomedullin-mediated signal transduction were greatly reduced. However, the loss of RCP did not effect CGRP binding or receptor density, indicating that RCP did not behave as a chaperone but was instead coupling the CGRP receptor to downstream effectors. A candidate CGRP receptor named calcitonin receptor-like receptor (CRLR) has been identified, and in this study RCP co-immunoprecipitated with CRLR indicating that these two proteins interact directly. Since CGRP and adrenomedullin can both signal through CRLR, which has been previously shown to require a chaperone protein for function, we now propose that a functional CGRP or adrenomedullin receptor consists of at least three proteins: the receptor (CRLR), the chaperone protein (RAMP), and RCP that couples the receptor to the cellular signal transduction pathway.

  7. Grout Impregnation of Pre-Placed Recycled Concrete Pavement (RCP) for Rapid Repair of Deteriorated Portland Cement Concrete Airfield Pavement

    Science.gov (United States)

    2007-04-01

    Hammitt (1985) also reports a high failure rate when using cold mix asphalt for crater repairs and recommended that it be abandoned as a technique...cement-treated base, embankment base material, and aggregate for asphalt paving mixtures . RCP has been used successfully for all of these types of...that recycled asphalt pavement ( RAP ) and RCP have a substantial history of usage in pavement construction. He reports that cost savings of 20 to

  8. Leptospira interrogans stably infects zebrafish embryos, altering phagocyte behavior and homing to specific tissues.

    Directory of Open Access Journals (Sweden)

    J Muse Davis

    Full Text Available Leptospirosis is an extremely widespread zoonotic infection with outcomes ranging from subclinical infection to fatal Weil's syndrome. Despite the global impact of the disease, key aspects of its pathogenesis remain unclear. To examine in detail the earliest steps in the host response to leptospires, we used fluorescently labelled Leptospira interrogans serovar Copenhageni to infect 30 hour post fertilization zebrafish embryos by either the caudal vein or hindbrain ventricle. These embryos have functional innate immunity but have not yet developed an adaptive immune system. Furthermore, they are optically transparent, allowing direct visualization of host-pathogen interactions from the moment of infection. We observed rapid uptake of leptospires by phagocytes, followed by persistent, intracellular infection over the first 48 hours. Phagocytosis of leptospires occasionally resulted in formation of large cellular vesicles consistent with apoptotic bodies. By 24 hours, clusters of infected phagocytes were accumulating lateral to the dorsal artery, presumably in early hematopoietic tissue. Our observations suggest that phagocytosis may be a key defense mechanism in the early stages of leptospirosis, and that phagocytic cells play roles in immunopathogenesis and likely in the dissemination of leptospires to specific target tissues.

  9. Characterization of a novel toxin-antitoxin module, VapBC, encoded by Leptospira interrogans chromosome

    Institute of Scientific and Technical Information of China (English)

    Yi Xuan ZHANG; Xiao Kui GUO; Chuan WU; Bo BI; Shuang Xi REN; Chun Fu WU; Guo Ping ZHAO

    2004-01-01

    Comparative genomic analysis of the coding sequences (CDSs) of Leptospira interrogans revealed a pair of closely linked genes homologous to the vapBC loci of many other bacteria with respect to both deduced amino acid sequences and operon organizations. Expression of single vapC gene in Escherichia coli resulted in inhibition of bacterial growth,whereas co-expression of vapBC restored the growth effectively. This phenotype is typical for three other characterized toxin-antitoxin systems of bacteria, i.e., mazEF[1], relBE[2] and chpIK[3]. The VapC proteins of bacteria and a thermophilic archeae, Solfolobus tokodaii, form a structurally distinguished group of toxin different from the other known toxins of bacteria. Phylogenetic analysis of both toxins and antitoxins of all categories indicated that although toxins were evolved from divergent sources and may or may not follow their speciation paths (as indicated by their 16s RNA sequences), co-evolution with their antitoxins was obvious.

  10. Three case studies involving Leptospira interrogans serovar pomona infection in mixed farming units.

    Science.gov (United States)

    Gummow, B; Myburgh, J G; Thompson, P N; van der Lugt, J J; Spencer, B T

    1999-03-01

    Three case studies involving Leptospira interrogans serovar pomona outbreaks within mixed farming systems in South Africa are described. On 2 farms, pigs constituted the main enterprise with cattle and sheep of secondary importance. On each of these 2 farms, abortion due to L. pomona in sows was confirmed by culture, and antibody titres to pomona were detected in cattle, sheep, horses and dogs. On the 3rd farm, a piggery was of secondary importance to cattle farming. Abortion and death in cows occurred on this farm and serology showed titres to various serovars, including pomona. L. pomona was also isolated from bovine urine, an aborted bovine foetus and kidneys from slaughtered pigs. This particular case study was regarded as clinically atypical in that adult Jersey cattle died of acute leptospirosis in a semiarid region of South Africa. In all 3 case studies, the poor management of pig effluent and of the drinking water and its sources played a pivotal role in the transmission of the disease. Inadequate vaccination of animals against Leptospira and poor record-keeping within the secondary farming enterprises were also contributing factors to the spread of leptospirosis.

  11. Sheep as maintenance host for Leptospira interrogans serovar hardjo subtype hardjobovis.

    Science.gov (United States)

    Gerritsen, M J; Koopmans, M J; Peterse, D; Olyhoek, T

    1994-09-01

    Transmission of Leptospira interrogans serovar hardjo subtype hardjobovis from naturally infected sheep to uninfected sheep and calves was studied. A microscopic agglutination test and ELISA were used to determine specific antibody responses in serum. Polymerase chain reaction was used to detect bacterial shedding in urine. Six sheep were derived from a dairy farm where cows were infected with L hardjobovis. Three of these sheep were seropositive for L hardjobovis, and 1 also shed leptospires in the urine. The other 2 sheep shed leptospires in the urine 7 days after the first observation date. The 6 sheep were placed on an isolated pasture together with a second group of 6 noninfected sheep. During the observation period of 140 days, 1 sheep of the second group became infected with L hardjobovis. On 5 consecutive days, a urine mixture from the 4 infected sheep was sprayed on the heads of 4 noninfected calves. Within 56 days, all calves that had been sprayed with urine shed L hardjobovis in the urine and became seropositive for L hardjobovis.

  12. Three case studies involving Leptospira interrogans serovar pomona infection in mixed farming units : case report

    Directory of Open Access Journals (Sweden)

    B. Gummow

    1999-07-01

    Full Text Available Three case studies involving Leptospira interrogans serovar pomona outbreaks within mixed farming systems in South Africa are described. On 2 farms, pigs constituted the main enterprise with cattle and sheep of secondary importance. On each of these 2 farms, abortion due to L. pomona in sows was confirmed by culture, and antibody titres to pomona were detected in cattle, sheep, horses and dogs. On the 3rd farm, a piggery was ofsecondary importance to cattle farming. Abortion and death in cows occurred on this farmand serology showed titres to various serovars, including pomona. L. pomona was also isolated from bovine urine, an aborted bovine foetus and kidneys from slaughtered pigs. This particular case study was regarded as clinically atypical in that adult Jersey cattle died of acute leptospirosis in a semiarid region of South Africa. In all 3 case studies, the poor management of pig effluent and of the drinking water and its sources played a pivotal role in the transmission of the disease. Inadequate vaccination of animals against Leptospira and poor record-keeping within the secondary farming enterprises were also contributing factors to the spread of leptospirosis.

  13. The Projection of Temperature and Precipitation over China under RCP Scenarios using a CMIP5 Multi-Model Ensemble

    Institute of Scientific and Technical Information of China (English)

    XU Chong-Hai; XU Ying

    2012-01-01

    Climate changes in 21st century China are described based on the projections of 11 climate models under Representative Concentration Pathway (RCP) scenarios. The results show that warming is expected in all regions of China under the RCP scenarios, with the northern regions showing greater warming than the southern regions. The warming tendency from 2011 to 2100 is 0.06°C/10 a for RCP2.6, 0.24°C/10 a for RCP4.5, and 0.63°C/10 a for RCP8.5. The projected time series of annual temperature have similar variation tendencies as the new greenhouse gas (GHG) emission scenario pathways, and the warming under the lower emission scenarios is less than under the higher emission scenarios. The regional averaged precipitation will increase, and the increasing precipitation in the northern regions is significant and greater than in the southern regions in China. It is noted that precipitation will tend to decrease in the southern parts of China during the period of 2011–2040, especially under RCP8.5. Compared with the changes over the globe and some previous projections, the increased warming and precipitation over China is more remarkable under the higher emission scenarios. The uncertainties in the projection are unavoidable, and further analyses are necessary to develop a better understanding of the future changes over the region.

  14. Management practices as risk factors for the presence of bulk milk antibodies to Salmonella, Neospora caninum and Leptospira interrogans serovar hardjo in Irish dairy herds.

    Science.gov (United States)

    O' Doherty, E; Berry, D P; O' Grady, L; Sayers, R

    2014-06-01

    A survey of management practices in 309 Irish dairy herds was used to identify risk factors for the presence of antibodies to Salmonella, Neospora caninum and Leptospira interrogans serovar hardjo in extensively managed unvaccinated dairy herds. A previous study documented a herd-level seroprevalence in bulk milk of 49%, 19% and 86% for Salmonella, Neospora caninum and leptospira interrogans serovar hardjo, respectively in the unvaccinated proportion of these 309 herds in 2009. Association analyses in the present study were carried out using multiple logistic regression models. Herds where cattle were purchased or introduced had a greater likelihood of being positive to leptospira interrogans serovar hardjo (PNeospora caninum (PNeospora caninum possibly due to limited access of dogs to infected materials including afterbirths. The information from this study will assist in the design of suitable control programmes for the diseases under investigation in pasture-based livestock systems.

  15. Genome-Wide Transcriptional Start Site Mapping and sRNA Identification in the Pathogen Leptospira interrogans

    Science.gov (United States)

    Zhukova, Anna; Fernandes, Luis Guilherme; Hugon, Perrine; Pappas, Christopher J.; Sismeiro, Odile; Coppée, Jean-Yves; Becavin, Christophe; Malabat, Christophe; Eshghi, Azad; Zhang, Jun-Jie; Yang, Frank X.; Picardeau, Mathieu

    2017-01-01

    Leptospira are emerging zoonotic pathogens transmitted from animals to humans typically through contaminated environmental sources of water and soil. Regulatory pathways of pathogenic Leptospira spp. underlying the adaptive response to different hosts and environmental conditions remains elusive. In this study, we provide the first global Transcriptional Start Site (TSS) map of a Leptospira species. RNA was obtained from the pathogen Leptospira interrogans grown at 30°C (optimal in vitro temperature) and 37°C (host temperature) and selectively enriched for 5′ ends of native transcripts. A total of 2865 and 2866 primary TSS (pTSS) were predicted in the genome of L. interrogans at 30 and 37°C, respectively. The majority of the pTSSs were located between 0 and 10 nucleotides from the translational start site, suggesting that leaderless transcripts are a common feature of the leptospiral translational landscape. Comparative differential RNA-sequencing (dRNA-seq) analysis revealed conservation of most pTSS at 30 and 37°C. Promoter prediction algorithms allow the identification of the binding sites of the alternative sigma factor sigma 54. However, other motifs were not identified indicating that Leptospira consensus promoter sequences are inherently different from the Escherichia coli model. RNA sequencing also identified 277 and 226 putative small regulatory RNAs (sRNAs) at 30 and 37°C, respectively, including eight validated sRNAs by Northern blots. These results provide the first global view of TSS and the repertoire of sRNAs in L. interrogans. These data will establish a foundation for future experimental work on gene regulation under various environmental conditions including those in the host. PMID:28154810

  16. Role of 72 kDa protein of Leptospira interrogans as a diagnostic marker in acute leptospirosis

    Directory of Open Access Journals (Sweden)

    M Riazi

    2014-01-01

    Full Text Available Background & objectives: Leptospirosis is a widespread zoonotic disease and a public health problem, particularly in tropical and subtropical countries. Varied clinical manifestations of the disease frequently lead to misdiagnosis resulting in life-threatening multi-organ complications. Therefore, early laboratory investigation using an appropriate diagnostic approach is crucial. In the present study, a potential protein marker was identified and evaluated for its usefulness in the serodiagnosis of acute leptospirosis. Methods: Leptospira interrogans serovar Icterohaemorrhagiae (L44, which represents a commonly prevalent serovar in Malaysia, was cultivated for preparation of sequential protein extract (SEQ. SDS-PAGE and immunoblotting were performed with a serum panel comprising confirmed cases of leptospirosis and controls (n=42 each. Identification and characterization of the highest scoring protein from the antigenic band was performed. Subsequently based on the nucleotide coding sequence of the protein, the corresponding recombinant protein was custom-produced. It was then evaluated for sensitivity and specificity by testing against 20 serum samples from leptospirosis patients and 32 from controls. Results: Among the antigenic components, a 72kDa protein band demonstrated significant sensitivity (83.3% and specificity (95.2% for the detection of specific anti-leptospiral IgM antibodies. The protein was identified by mass-spectrometry analysis as heat shock protein DnaK of L. interrogans. Recombinant form of the protein (r72SEQ showed 85 per cent sensitivity and 81 per cent specificity for the detection of specific anti-leptospiral IgM antibodies. Interpretation & conclusions: The findings of our study indicate that a protein (72kDa of L. interrogans has the potential utility of being used for the diagnosis of acute leptospirosis. Further studies need to be done to confirm these findings.

  17. Seasonal prevalence of antibodies to Leptospira interrogans in Antillean manatees from a landlocked lake in Tabasco, Mexico.

    Science.gov (United States)

    Aragón-Martínez, Arianna; Olivera-Gómez, León D; Jiménez-Domínguez, Darwin

    2014-07-01

    Factors that alter the dynamics of ecologic systems can influence transmission of infectious diseases and may lead to decreases in natural populations. Leptospirosis is a cosmopolitan disease of zoonotic importance that affects most mammals. At the southern Gulf of Mexico, Antillean manatees (Trichechus manatus manatus) inhabit highly variable environments, with extended floods during the rainy season and drought conditions during the dry season that affect food availability and the thermal environment for manatees. We tested for changes in prevalence and titers of antibodies to 12 serovars of Leptospira interrogans in manatees between dry and rainy seasons. We determined titers for L. interrogans through microscopic agglutination tests (MAT) from 10 manatees, six during the dry season (DS), and six during the rainy season (RS) in Laguna de las Ilusiones, a landlocked lake hosting a population of about 20 manatees. All individuals were antibody positive (titers ≥ 100) to at least one serovar. The serovars bataviae, bratislava, canicola, and icterohaemorrhagiae had overall prevalences ≥ 50%; bataviae, bratislava, and canicola had prevalences ≥ 50% during both seasons. Serovars icterohaemorrhagiae and pyrogenes had prevalences ≥ 50% during DS and pomona, tarassovi, wolfii, and autumnalis during RS. Significant differences in prevalence between seasons were found for pomona, tarassovi, and autumnalis. Titers of tarassovi, wolfii, autumnalis, and bataviae were significantly higher during RS. There was a high prevalence of L. interrogans during the RS independent of high availability of plant foods, coinciding with the epizootiology of the bacteria that are endemic to tropical regions. Another factor possibly influencing prevalence is high anthropogenic pressure at the lake, causing an increase in potential sources of infection. Because of possible cross-reaction in MAT, further research is needed on the molecular discrimination of serovars in animals in the

  18. Crystal structures of Leptospira interrogans FAD-containing ferredoxin-NADP+ reductase and its complex with NADP+.

    Science.gov (United States)

    Nascimento, Alessandro S; Catalano-Dupuy, Daniela L; Bernardes, Amanda; Neto, Mario de Oliveira; Santos, Maria Auxiliadora M; Ceccarelli, Eduardo A; Polikarpov, Igor

    2007-10-24

    Ferredoxin-NADP(H) reductases (FNRs) are flavoenzymes that catalyze the electron transfer between NADP(H) and the proteins ferredoxin or flavodoxin. A number of structural features distinguish plant and bacterial FNRs, one of which is the mode of the cofactor FAD binding. Leptospira interrogans is a spirochaete parasitic bacterium capable of infecting humans and mammals in general. Leptospira interrogans FNR (LepFNR) displays low sequence identity with plant (34% with Zea mays) and bacterial (31% with Escherichia coli) FNRs. However, LepFNR contains all consensus sequences that define the plastidic class FNRs. The crystal structures of the FAD-containing LepFNR and the complex of the enzyme with NADP+, were solved and compared to known FNRs. The comparison reveals significant structural similarities of the enzyme with the plastidic type FNRs and differences with the bacterial enzymes. Our small angle X-ray scattering experiments show that LepFNR is a monomeric enzyme. Moreover, our biochemical data demonstrate that the LepFNR has an enzymatic activity similar to those reported for the plastidic enzymes and that is significantly different from bacterial flavoenzymes, which display lower turnover rates. LepFNR is the first plastidic type FNR found in bacteria and, despite of its low sequence similarity with plastidic FNRs still displays high catalytic turnover rates. The typical structural and biochemical characteristics of plant FNRs unveiled for LepFNR support a notion of a putative lateral gene transfer which presumably offers Leptospira interrogans evolutionary advantages. The wealth of structural information about LepFNR provides a molecular basis for advanced drugs developments against leptospirosis.

  19. A highly stable plastidic-type ferredoxin-NADP(H reductase in the pathogenic bacterium Leptospira interrogans.

    Directory of Open Access Journals (Sweden)

    Daniela L Catalano-Dupuy

    Full Text Available Leptospira interrogans is a bacterium that is capable of infecting animals and humans, and its infection causes leptospirosis with a range of symptoms from flu-like to severe illness and death. Despite being a bacteria, Leptospira interrogans contains a plastidic class ferredoxin-NADP(H reductase (FNR with high catalytic efficiency, at difference from the bacterial class FNRs. These flavoenzymes catalyze the electron transfer between NADP(H and ferredoxins or flavodoxins. The inclusion of a plastidic FNR in Leptospira metabolism and in its parasitic life cycle is not currently understood. Bioinformatic analyses of the available genomic and proteins sequences showed that the presence of this enzyme in nonphotosynthetic bacteria is restricted to the Leptospira genus and that a [4Fe-4S] ferredoxin (LB107 encoded by the Leptospira genome may be the natural substrate of the enzyme. Leptospira FNR (LepFNR displayed high diaphorase activity using artificial acceptors and functioned as a ferric reductase. LepFNR displayed cytochrome c reductase activity with the Leptospira LB107 ferredoxin with an optimum at pH 6.5. Structural stability analysis demonstrates that LepFNR is one of the most stable FNRs analyzed to date. The persistence of a native folded LepFNR structure was detected in up to 6 M urea, a condition in which the enzyme retains 38% activity. In silico analysis indicates that the high LepFNR stability might be due to robust interactions between the FAD and the NADP(+ domains of the protein. The limited bacterial distribution of plastidic class FNRs and the biochemical and structural properties of LepFNR emphasize the uniqueness of this enzyme in the Leptospira metabolism. Our studies show that in L. interrogans a plastidic-type FNR exchanges electrons with a bacterial-type ferredoxin, process which has not been previously observed in nature.

  20. A highly stable plastidic-type ferredoxin-NADP(H) reductase in the pathogenic bacterium Leptospira interrogans.

    Science.gov (United States)

    Catalano-Dupuy, Daniela L; Musumeci, Matías A; López-Rivero, Arleth; Ceccarelli, Eduardo A

    2011-01-01

    Leptospira interrogans is a bacterium that is capable of infecting animals and humans, and its infection causes leptospirosis with a range of symptoms from flu-like to severe illness and death. Despite being a bacteria, Leptospira interrogans contains a plastidic class ferredoxin-NADP(H) reductase (FNR) with high catalytic efficiency, at difference from the bacterial class FNRs. These flavoenzymes catalyze the electron transfer between NADP(H) and ferredoxins or flavodoxins. The inclusion of a plastidic FNR in Leptospira metabolism and in its parasitic life cycle is not currently understood. Bioinformatic analyses of the available genomic and proteins sequences showed that the presence of this enzyme in nonphotosynthetic bacteria is restricted to the Leptospira genus and that a [4Fe-4S] ferredoxin (LB107) encoded by the Leptospira genome may be the natural substrate of the enzyme. Leptospira FNR (LepFNR) displayed high diaphorase activity using artificial acceptors and functioned as a ferric reductase. LepFNR displayed cytochrome c reductase activity with the Leptospira LB107 ferredoxin with an optimum at pH 6.5. Structural stability analysis demonstrates that LepFNR is one of the most stable FNRs analyzed to date. The persistence of a native folded LepFNR structure was detected in up to 6 M urea, a condition in which the enzyme retains 38% activity. In silico analysis indicates that the high LepFNR stability might be due to robust interactions between the FAD and the NADP(+) domains of the protein. The limited bacterial distribution of plastidic class FNRs and the biochemical and structural properties of LepFNR emphasize the uniqueness of this enzyme in the Leptospira metabolism. Our studies show that in L. interrogans a plastidic-type FNR exchanges electrons with a bacterial-type ferredoxin, process which has not been previously observed in nature.

  1. Crystal structures of Leptospira interrogans FAD-containing ferredoxin-NADP+ reductase and its complex with NADP+

    Directory of Open Access Journals (Sweden)

    Ceccarelli Eduardo A

    2007-10-01

    Full Text Available Abstract Background Ferredoxin-NADP(H reductases (FNRs are flavoenzymes that catalyze the electron transfer between NADP(H and the proteins ferredoxin or flavodoxin. A number of structural features distinguish plant and bacterial FNRs, one of which is the mode of the cofactor FAD binding. Leptospira interrogans is a spirochaete parasitic bacterium capable of infecting humans and mammals in general. Leptospira interrogans FNR (LepFNR displays low sequence identity with plant (34% with Zea mays and bacterial (31% with Escherichia coli FNRs. However, LepFNR contains all consensus sequences that define the plastidic class FNRs. Results The crystal structures of the FAD-containing LepFNR and the complex of the enzyme with NADP+, were solved and compared to known FNRs. The comparison reveals significant structural similarities of the enzyme with the plastidic type FNRs and differences with the bacterial enzymes. Our small angle X-ray scattering experiments show that LepFNR is a monomeric enzyme. Moreover, our biochemical data demonstrate that the LepFNR has an enzymatic activity similar to those reported for the plastidic enzymes and that is significantly different from bacterial flavoenzymes, which display lower turnover rates. Conclusion LepFNR is the first plastidic type FNR found in bacteria and, despite of its low sequence similarity with plastidic FNRs still displays high catalytic turnover rates. The typical structural and biochemical characteristics of plant FNRs unveiled for LepFNR support a notion of a putative lateral gene transfer which presumably offers Leptospira interrogans evolutionary advantages. The wealth of structural information about LepFNR provides a molecular basis for advanced drugs developments against leptospirosis.

  2. Effects of Static Magnetic Field on Growth of Leptospire, Leptospira interrogans serovar canicola: Immunoreactivity and Cell Division

    CERN Document Server

    Triampo, W; Triampo, D; Wong-Ekkabut, J; Tang, I M; Triampo, Wannapong; Doungchawee, Galayanee; Triampo, Darapond; Wong-Ekkabut, Jirasak

    2004-01-01

    The effects of the exposure of the bacterium, Leptospira interrogans serovar canicola to a constant magnetic field with magnetic flux density from a permanent ferrite magnet = 140 mT were studied. Changes in Leptospira cells after their exposure to the field were determined on the basis of changes in their growth behavior and agglutination immunoreactivity with a homologous antiserum using darkfield microscopy together with visual imaging. The data showed that the exposed Leptospira cells have lower densities and lower agglutination immunoreactivity than the unexposed control group. Interestingly, some of the exposed Leptospira cells showed abnormal morphologies such as large lengths. We discussed some of the possible reasons for these observations.

  3. Minilaparotomy radical cystoprostatectomy (Minilap RCP) in the surgical management of urinary bladder carcinoma: early experience.

    Science.gov (United States)

    Rawal, Sudhir; Raghunath, S K; Khanna, Samir; Jain, Deepak; Kaul, Rakesh; Kumar, Prem; Chhabra, Rakesh; Bhushan, Kirti

    2008-09-01

    To assess the feasibility of minilaparotomy for radical cystoprostatectomy (RCP) and urinary diversion in the management of urinary bladder carcinoma. A total of 45 consecutive patients with muscle invasive urinary bladder cancer underwent RCP and urinary diversion [32, 12 and 1 patients with pitchers pot orthotopic neobladder (NB), ileal conduit (IC) and sigma rectal pouch respectively], between May 2006 and June 2007, using 8-12 cm infraumbilical midline vertical incision from pubic symphysis, were prospectively analyzed for technical feasibility, operative time, blood loss, intraoperative and postoperative complications and return of bowel function. All the patients were males with average age of 59.65 years (44-79 years) and average body mass index of 23.97(17.7-29.5). The length of the incision was 8, 10 and 12 cm in 4, 39 and 2 patients, respectively. The average number of lymph nodes removed on the right and left side was 14 and 16, respectively. The average blood loss was 1046 ml (595-2100 ml). Return of bowel sounds was observed on an average by second postoperative day (1-5 days). Average postoperative stay was 14 days (range 10-24 days) for NB and 7 days (6-8 days) for IC patients. One (2.22%) patient died on the 18th postoperative day due to septicemia and acute renal failure. Minilaparotomy RCP is technically feasible without compromising the oncological principles. Complete removal of urachus and adequate clearance of pelvic lymph nodes is not difficult with the small incision. Early restoration of bowel function, early postoperative recovery and good cosmesis seem to be the main advantages.

  4. NASA uses Eclipse RCP Applications for Experiments on the International Space Station

    Science.gov (United States)

    Cohen, Tamar

    2013-01-01

    Eclipse is going to space for the first time in 2013! The International Space Station (ISS) is used as a site for experiments any software developed as part of these experiments has to comply with extensive and strict user interface guidelines. NASA Ames Research Center's Intelligent Robotics Group is doing 2 sets of experiments, both with astronauts using Eclipse RCP applications to remotely control robots. One experiment will control SPHERES with an Android Smartphone on the ISS the other experiment will control a K10 rover on Earth.

  5. Adipose tissue is the first colonization site of Leptospira interrogans in subcutaneously infected hamsters

    Science.gov (United States)

    Ozuru, Ryo; Saito, Mitsumasa; Kanemaru, Takaaki; Miyahara, Satoshi; Villanueva, Sharon Y. A. M.; Murray, Gerald L.; Adler, Ben; Fujii, Jun; Yoshida, Shin-ichi

    2017-01-01

    Leptospirosis is one of the most widespread zoonoses in the world, and its most severe form in humans, “Weil’s disease,” may lead to jaundice, hemorrhage, renal failure, pulmonary hemorrhage syndrome, and sometimes,fatal multiple organ failure. Although the mechanisms underlying jaundice in leptospirosis have been gradually unraveled, the pathophysiology and distribution of leptospires during the early stage of infection are not well understood. Therefore, we investigated the hamster leptospirosis model, which is the accepted animal model of human Weil’s disease, by using an in vivo imaging system to observe the whole bodies of animals infected with Leptospira interrogans and to identify the colonization and growth sites of the leptospires during the early phase of infection. Hamsters, infected subcutaneously with 104 bioluminescent leptospires, were analyzed by in vivo imaging, organ culture, and microscopy. The results showed that the luminescence from the leptospires spread through each hamster’s body sequentially. The luminescence was first detected at the injection site only, and finally spread to the central abdomen, in the liver area. Additionally, the luminescence observed in the adipose tissue was the earliest detectable compared with the other organs, indicating that the leptospires colonized the adipose tissue at the early stage of leptospirosis. Adipose tissue cultures of the leptospires became positive earlier than the blood cultures. Microscopic analysis revealed that the leptospires colonized the inner walls of the blood vessels in the adipose tissue. In conclusion, this is the first study to report that adipose tissue is an important colonization site for leptospires, as demonstrated by microscopy and culture analyses of adipose tissue in the hamster model of Weil’s disease. PMID:28245231

  6. Protection of Guinea Pigs against Leptospira interrogans Serovar Lai by LipL21 DNA Vaccine

    Institute of Scientific and Technical Information of China (English)

    Hanjiang He; Wenyu Wang; Zhongdao Wu; Zhiyue Lv; Jun Li; Lizhi Tan

    2008-01-01

    In this study,the full lipL21 gene fragment encoding outer membrane protein LipL21 was cloned from L. Interrogans serovar Lai and inserted into eukaryotic expression vector pcDNA3.1(+).The guinea pigs were immunized with pcDNA3.1(+)-lipL21,pcDNA3.1(+) or PBS.Six weeks after the second immunization,the splenocytes were isolated to detect their proliferative ability by lymphocyte transformation experiments.In addition,microscopic agglutination test was used for quantitative detection of specific antibodies.The rest guinea pigs were challenged intraperitoneally with L.interogans sorevar Lai.Then,protective effect was evaluated on the basis of survival and histopathological lesions in the kidneys,lungs,and liver.The lipL21 gene Was successfully expressed in COS-7 cells through recombinant pcDNA3.1(+)-lipL21.The titer of specific antibodies substantially increased,and the stimulation index of splenocytes increased significantly.Hence,the pcDNA3.1(+)-lipL21 could protect the immunized guinea pigs from homotypic Leptospira infection.Furthermore,no obvious pathologic changes were observed in the pcDNA3.1(+)-lipL21 immunized guinea pigs.The results showed that the protective effect with pathogenic strains of Leptospira was shared by LipL21 mediated through a plasmid vector. Consequently,these results indicated that the lipL21 DNA vaccine Was a promising candidate for the prevention of leptospirosis.

  7. Identification and partial characterization of a novel hemolysin from Leptospira interrogans serovar lai.

    Science.gov (United States)

    Lee, S H; Kim, K A; Park, Y G; Seong, I W; Kim, M J; Lee, Y J

    2000-08-22

    It has been suggested that leptospiral hemolysins are important in the virulence and pathogenesis of leptospirosis. We have isolated an Escherichia coli clone carrying the 7.8kb DNA insert from a genomic library of Leptospira interrogans serovar lai by plaque hybridization using a sequence derived from the sphingomyelinase C gene (sphA) of L. borgpetersenii. The clone showed a clear beta-hemolytic zone on sheep blood agar and high hemolytic activities on both human and sheep erythrocytes in liquid assays. The clone carried at least two genes responsible for the hemolytic activities, encoded by two open reading frames of 1662 and 816 nucleotides, which are named sphH and hap-1 (hemolysis associated protein-1), respectively. The SphH showed 75% homology to the SphA at the amino acid level, and the Hap-1 showed no significant homology in major databases. Interestingly, however, E. coli cells harboring sphH did not show sphingomyelinase or phospholipase activities. Moreover, SphH-mediated hemolysis was osmotically protected by polyethylene glycol 5000, suggesting that the hemolysis is likely to be caused by pore formation on the membrane. The SphH was successfully expressed in E. coli as a histidine (His)-SphH fusion protein. Both sphH and hap-1 were highly conserved among the Leptospira species, except for the absence of sphH in non-pathogenic L. biflexa serovar patoc. We concluded that the SphH is a novel hemolysin of a pathogenic Leptospira species, which may be a putative pore-forming protein.

  8. Expression of calcitonin gene-related peptide-receptor component protein (CGRP-RCP) in human myometrium in differing physiological states and following misoprostol administration.

    Science.gov (United States)

    Goharkhay, Nina; Lu, Jing; Felix, Juan C; Wing, Deborah A

    2007-09-01

    Our objective was to assess relative expression levels of mRNA for calcitonin gene-related peptide-receptor component protein (CGRP-RCP) in human myometrium in various physiological states. Using semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR), we analyzed myometrial samples from 46 women (10 menopausal, 10 nongravid premenopausal, 19 gravidae, and 7 premenopausal misoprostol-treated nongravid women) for the specific expression of CGRP-RCP mRNA. The expression of CGRP-RCP was significantly increased in gravid compared with nongravid myometrium ( P RCP expression were found among the other study groups. We concluded that the increased mRNA expression CGRP-RCP in gravid myometrium supports the possibility of involvement of CGRP in the control of myometrial contractility. Additional studies are necessary to evaluate the exact mechanism of action of CGRP and CGRP-RCP in human myometrium.

  9. FIP1/RCP binding to Golgin-97 regulates retrograde transport from recycling endosomes to the trans-Golgi network.

    Science.gov (United States)

    Jing, Jian; Junutula, Jagath R; Wu, Christine; Burden, Jemima; Matern, Hugo; Peden, Andrew A; Prekeris, Rytis

    2010-09-01

    Many proteins are retrieved to the trans-Golgi Network (TGN) from the endosomal system through several retrograde transport pathways to maintain the composition and function of the TGN. However, the molecular mechanisms involved in these distinct retrograde pathways remain to be fully understood. Here we have used fluorescence and electron microscopy as well as various functional transport assays to show that Rab11a/b and its binding protein FIP1/RCP are both required for the retrograde delivery of TGN38 and Shiga toxin from early/recycling endosomes to the TGN, but not for the retrieval of mannose-6-phosphate receptor from late endosomes. Furthermore, by proteomic analysis we identified Golgin-97 as a FIP1/RCP-binding protein. The FIP1/RCP-binding domain maps to the C-terminus of Golgin-97, adjacent to its GRIP domain. Binding of FIP1/RCP to Golgin-97 does not affect Golgin-97 recruitment to the TGN, but appears to regulate the targeting of retrograde transport vesicles to the TGN. Thus, we propose that FIP1/RCP binding to Golgin-97 is required for tethering and fusion of recycling endosome-derived retrograde transport vesicles to the TGN.

  10. Analysis of the change in temperature trends in Subansiri River basin for RCP scenarios using CMIP5 datasets

    Science.gov (United States)

    Shivam; Goyal, Manish Kumar; Sarma, Arup Kumar

    2017-08-01

    This study focuses on changes in the maximum and minimum temperature over the Subansiri River basin for different climate change scenarios. For the study, dataset from Intergovernmental Panel on Climate Change (IPCC) fifth assessment report (AR5) (i.e., coupled model intercomparison project phase five (CMIP5) dataset with representative concentration pathway (RCP) scenarios) were utilized. Long-term (2011-2100) maximum temperature ( T max) and minimum temperature (Tmin) time series were generated using the statistical downscaling technique for low emission scenario (RCP2.6), moderate emission scenario (RCP6.0), and extreme emission scenario (RCP8.5). Trends and change of magnitude in T max, T min, and diurnal temperature range (DTR) were analyzed for different interdecadal time scales (2011-2100, 2011-2040, 2041-2070, 2070-2100) using Mann-Kendall non-parametric test and Sen's slope estimator, respectively. The temperature data series for the observed duration (1981-2000) has been found to show increasing trends in T max and T min at both annual and monthly scale. Trend analysis of downscaled temperature for the period 2011-2100 shows increase in annual maximum temperature and annual minimum temperature for all the selected RCP scenarios; however, on the monthly scale, T max and T min have been seen to have decreasing trends in some months.

  11. Analysis of the change in temperature trends in Subansiri River basin for RCP scenarios using CMIP5 datasets

    Science.gov (United States)

    Shivam; Goyal, Manish Kumar; Sarma, Arup Kumar

    2016-06-01

    This study focuses on changes in the maximum and minimum temperature over the Subansiri River basin for different climate change scenarios. For the study, dataset from Intergovernmental Panel on Climate Change (IPCC) fifth assessment report (AR5) (i.e., coupled model intercomparison project phase five (CMIP5) dataset with representative concentration pathway (RCP) scenarios) were utilized. Long-term (2011-2100) maximum temperature (T max) and minimum temperature (Tmin) time series were generated using the statistical downscaling technique for low emission scenario (RCP2.6), moderate emission scenario (RCP6.0), and extreme emission scenario (RCP8.5). Trends and change of magnitude in T max, T min, and diurnal temperature range (DTR) were analyzed for different interdecadal time scales (2011-2100, 2011-2040, 2041-2070, 2070-2100) using Mann-Kendall non-parametric test and Sen's slope estimator, respectively. The temperature data series for the observed duration (1981-2000) has been found to show increasing trends in T max and T min at both annual and monthly scale. Trend analysis of downscaled temperature for the period 2011-2100 shows increase in annual maximum temperature and annual minimum temperature for all the selected RCP scenarios; however, on the monthly scale, T max and T min have been seen to have decreasing trends in some months.

  12. RCP: a novel probe design bias correction method for Illumina Methylation BeadChip.

    Science.gov (United States)

    Niu, Liang; Xu, Zongli; Taylor, Jack A

    2016-09-01

    The Illumina HumanMethylation450 BeadChip has been extensively utilized in epigenome-wide association studies. This array and its successor, the MethylationEPIC array, use two types of probes-Infinium I (type I) and Infinium II (type II)-in order to increase genome coverage but differences in probe chemistries result in different type I and II distributions of methylation values. Ignoring the difference in distributions between the two probe types may bias downstream analysis. Here, we developed a novel method, called Regression on Correlated Probes (RCP), which uses the existing correlation between pairs of nearby type I and II probes to adjust the beta values of all type II probes. We evaluate the effect of this adjustment on reducing probe design type bias, reducing technical variation in duplicate samples, improving accuracy of measurements against known standards, and retention of biological signal. We find that RCP is statistically significantly better than unadjusted data or adjustment with alternative methods including SWAN and BMIQ. We incorporated the method into the R package ENmix, which is freely available from the Bioconductor website (https://www.bioconductor.org/packages/release/bioc/html/ENmix.html). niulg@ucmail.uc.edu Supplementary data are available at Bioinformatics online. Published by Oxford University Press 2016. This work is written by US Government employees and is in the public domain in the US.

  13. Characterization of a balanced reciprocal translocation, rcp(9;11)(q27;q11) in cattle.

    Science.gov (United States)

    De Lorenzi, L; De Giovanni, A; Molteni, L; Denis, C; Eggen, A; Parma, P

    2007-01-01

    Cytogenetic analysis of a phenotypically normal young bull from Marchigiana breed revealed the presence of an abnormal karyotype. The observation of longer and smaller chromosomes than BTA1 and BTA29, respectively in all metaphases suggested the presence of a reciprocal translocation. RBG-banding confirmed this hypothesis revealing the involvement of BTA9 and BTA11. FISH analyses using cattle-specific BAC clones (474A12 and 293G09 for BTA9; 035D03 for BTA11) identified rcp(9;11)(q27;q11) in the two regions affected. Moreover analyses performed on both parents established the 'de novo' origin of the anomaly. Comparison with human homologue sequences (HSA6q24.3-->q25.3 for BTA9q27 and HSA2q11.1-->q12.1 for BTA11q11) revealed that both breakpoint regions are gene rich as up to date at least 200 genes have been localized in these regions. Thus, further analyses are required to identify the sequences disrupted by the breakpoints and to verify their consequences on rcp carrier phenotype. Copyright (c) 2008 S. Karger AG, Basel.

  14. Predictable Technique to Register Retruded Contact Position (RCP) Using a Disposable Jaw Relation Recording Device.

    Science.gov (United States)

    Daher, Tony; Lobel, William A; Massad, Joseph; Ahuja, Swati; Danilov, Zarko Jack

    2015-05-01

    The dental literature presents various definitions and techniques to describe and register centric relation (CR) or centric occlusion (CO). Briefly reviewing the literature in relation to CR, this article proposes the use of the term retruded contact position (RCP), clinically defined as retruded, unstrained, repeatable position and where the mandibular movements start when a Gothic arch tracing is used. With this clinical definition, a technique can be easily selected that meets all the requirements of such position. The article discusses the use of a jaw recorder that is an intraorally graphic recording device that results in a tracing of mandibular movements in one plane, with the apex of the tracing indicating the retruded, unstrained, and repeatable relationship. The intersection of the arcs produced by the right and left working movement form the apex of the Gothic arch tracing. Several clinical situations using the jaw recorder are described. Clinicians can now quickly and accurately record RCP, balance complete, partial, or implant dentures, and orthopedically reposition the mandible. The technique achieves highly reliable and reproducible results.

  15. Pre-test analysis of ATLAS SBO with RCP seal leakage scenario using MARS code

    Energy Technology Data Exchange (ETDEWEB)

    Pham, Quang Huy; Lee, Sang Young; Oh, Seung Jong [KEPCO International Nuclear Graduate School, Ulsan (Korea, Republic of)

    2015-10-15

    This study presents a pre-test calculation for the Advanced Thermal-hydraulic Test Loop for Accident Simulation (ATLAS) SBO experiment with RCP seal leakage scenario. Initially, turbine-driven auxfeed water pumps are used. Then, outside cooling water injection method is used for long term cooling. The analysis results would be useful for conducting the experiment to verify the APR 1400 extended SBO optimum mitigation strategy using outside cooling water injection in future. The pre-test calculation for ATLAS extended SBO with RCP seal leakage and outside cooling water injection scenario is performed. After Fukushima nuclear accident, the capability of coping with the extended station blackout (SBO) becomes important. Many NPPs are applying FLEX approach as main coping strategies for extended SBO scenarios. In FLEX strategies, outside cooling water injection to reactor cooling system (RCS) and steam generators (SGs) is considered as an effective method to remove residual heat and maintain the inventory of the systems during the accident. It is worthwhile to examine the soundness of outside cooling water injection method for extended SBO mitigation by both calculation and experimental demonstration. From the calculation results, outside cooling water injection into RCS and SGs is verified as an effective method during extended SBO when RCS and SGs depressurization is sufficiently performed.

  16. PROTECTION AGAINST LEPTOSPIROSIS BY IMMUNIZATION WITH PLASMID DNA ENCODING 33 kDa ENDOFLAGELLIN OF L.INTERROGANS SEROVAR LAI

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Objective. To evaluate how the efficacy of DNA inocutation affects the ability to raise protective immunity against Leptospira.Methods. A pair of oligonucleotide primers were designed to amplify the endoflagellar gene of L. interrogans sensu stricto serovar lai. An approximately 840bp fragment was generated with PCR and inserted into VR1012, a plasmid DNA expression vector, after the fragment and VR1012 were digested respectively with EcoRV and Sal I. A recombinant plasmid designated as VR1012+flaB2 was obtained. The vector, VR1012 consits of a pUC18 backbone with the cytomegalovirus(CMV) IE1 enhancer, promoter, and intron A, transcription regulatory elements and the BGH polyadenylation sequences driving the expressing of leptospiral endoflagellar gene of L. interrogans sensu stricto serovar lai. Plasmid encoding leptospiral endoflagellin gene was injected into quadriceps of NZW rabbits.Results.This resulted in the generation of specific leptospiral antibody with high ELISA titer (1:32768) in the rabbits. Immuno/protection was performed in guinea pigs without adjuvant. The group"VR1012+flaB2" showed higher survival rate(90%,9/10 animals),compared with the group "VR1012 lack flaB2" and the group "normal saline".Conclusion.The technique of DNA vaccine has potential advantages over certain other vaccine preparation technologies. However whether DNA vaccine will be useful for vaccine development remains to be tested.

  17. Draft genome of the Leptospira interrogans strains, Acegua, RCA, Prea, and Capivara, obtained from wildlife maintenance hosts and infected domestic animals

    Science.gov (United States)

    Kremer, Frederico S; Eslabão, Marcus R; Jorge, Sérgio; Oliveira, Natasha R; Labonde, Julia; Santos, Monize NP; Monte, Leonardo G; Grassmann, André A; Cunha, Carlos EP; Forster, Karine M; Moreno, Luísa Z; Moreno, Andrea M; Campos, Vinicius F; McBride, Alan JA; Pinto, Luciano S; Dellagostin, Odir A

    2016-01-01

    In the present paper, we announce new draft genomes of four Leptospira interrogans strains named Acegua, RCA, Prea, and Capivara. These strains were isolated in the state of Rio Grande do Sul, Brazil, from cattle, dog, Brazilian guinea pig, and capybara, respectively. PMID:27074260

  18. Draft genome of the Leptospira interrogans strains, Acegua, RCA, Prea, and Capivara, obtained from wildlife maintenance hosts and infected domestic animals

    Directory of Open Access Journals (Sweden)

    Frederico S Kremer

    2016-04-01

    Full Text Available In the present paper, we announce new draft genomes of four Leptospira interrogans strains named Acegua, RCA, Prea, and Capivara. These strains were isolated in the state of Rio Grande do Sul, Brazil, from cattle, dog, Brazilian guinea pig, and capybara, respectively.

  19. Whole-Genome Sequence of Leptospira interrogans Serovar Hardjo Subtype Hardjoprajitno Strain Norma, Isolated from Cattle in a Leptospirosis Outbreak in Brazil.

    Science.gov (United States)

    Cosate, M R V; Soares, S C; Mendes, T A; Raittz, R T; Moreira, E C; Leite, R; Fernandes, G R; Haddad, J P A; Ortega, J Miguel

    2015-11-05

    Leptospirosis is caused by pathogenic bacteria of the genus Leptospira spp. This neglected re-emergent disease has global distribution and relevance in veterinary production. Here, we report the whole-genome sequence and annotation of Leptospira interrogans serovar Hardjo subtype Hardjoprajitno strain Norma, isolated from cattle in a livestock leptospirosis outbreak in Brazil.

  20. Draft genome of the Leptospira interrogans strains, Acegua, RCA, Prea, and Capivara, obtained from wildlife maintenance hosts and infected domestic animals

    OpenAIRE

    Kremer,Frederico S; Eslabão,Marcus R; Sérgio Jorge; Oliveira, Natasha R; Julia Labonde; Monize NP Santos; Monte, Leonardo G.; André A Grassmann; Carlos EP Cunha; Karine M. Forster; Moreno, Luísa Z; Moreno,Andrea M.; Campos, Vinicius F; Alan JA McBride; Pinto, Luciano S.

    2016-01-01

    In the present paper, we announce new draft genomes of four Leptospira interrogans strains named Acegua, RCA, Prea, and Capivara. These strains were isolated in the state of Rio Grande do Sul, Brazil, from cattle, dog, Brazilian guinea pig, and capybara, respectively.

  1. Prevalence of Leptospira interrogans antibodies in free-ranging Tayassu pecari of the Southern Pantanal, Brazil, an ecosystem where wildlife and cattle interact.

    Science.gov (United States)

    de Freitas, Tatiana P Tavares; Keuroghlian, Alexine; Eaton, Donald P; de Freitas, Emanuel Barbosa; Figueiredo, Aline; Nakazato, Luciano; de Oliveira, Jacqueline M; Miranda, Flávia; Paes, Rita Cassia S; Monteiro, Leticia A R Carneiro; Lima, José Vergílio B; da C Neto, Aparecida A; Dutra, Valéria; de Freitas, Julio Cesar

    2010-12-01

    We surveyed a wild population of white-lipped peccaries (Tayassu pecari) in the Brazilian Pantanal for evidence of Leptospira interrogans. Serum samples from 71 free-ranging T. pecari were obtained between 2003 and 2005 in the southern Pantanal of Mato Grosso do Sul state. We used microscopic microagglutination to test for antibodies against 14 L. interrogans serovars (antibody titers ≥ 1:100 were considered seropositive). Seventy percent of captured animals tested positive for leptospirosis antibodies. Antibodies against icterohaemorrhagiae and autumnalis serovars were the most prevalent. We used log-linear analyses to test for associations among seropositivity, age class, and sex of captured animals. Seropositivity was strongly associated with animal age class, but independent of sex. Forty-six percent of animals less than 2 years old, 63% of adults during peak reproductive years, and 100% of the oldest age class were seropositive. A nonparametric multivariate procedure (MRPP) showed that the composition of serovar antibody types changed with age, and ANOVA models demonstrated that antibody titers increased with age, suggesting long-term exposure to a greater number and variety (i.e., serovar types) of L. interrogans infections. This study presents the first quantitative survey of antibodies against L. interrogans serovars in a T. pecari population of the Pantanal. The high prevalence of leptospirosis antibodies in free-ranging white-lipped peccaries and the potential impacts on reproduction and population dynamics emphasize the need for further studies investigating the roles of Pantanal wildlife and livestock in the transmission and maintenance of L. interrogans in the environment.

  2. An Rpb4/Rpb7-like complex in yeast RNA polymerase III contains the orthologue of mammalian CGRP-RCP.

    Science.gov (United States)

    Siaut, Magali; Zaros, Cécile; Levivier, Emilie; Ferri, Maria-Laura; Court, Magali; Werner, Michel; Callebaut, Isabelle; Thuriaux, Pierre; Sentenac, André; Conesa, Christine

    2003-01-01

    The essential C17 subunit of yeast RNA polymerase (Pol) III interacts with Brf1, a component of TFIIIB, suggesting a role for C17 in the initiation step of transcription. The protein sequence of C17 (encoded by RPC17) is conserved from yeasts to humans. However, mammalian homologues of C17 (named CGRP-RCP) are known to be involved in a signal transduction pathway related to G protein-coupled receptors, not in transcription. In the present work, we first establish that human CGRP-RCP is the genuine orthologue of C17. CGRP-RCP was found to functionally replace C17 in Deltarpc17 yeast cells; the purified mutant Pol III contained CGRP-RCP and had a decreased specific activity but initiated faithfully. Furthermore, CGRP-RCP was identified by mass spectrometry in a highly purified human Pol III preparation. These results suggest that CGRP-RCP has a dual function in mammals. Next, we demonstrate by genetic and biochemical approaches that C17 forms with C25 (encoded by RPC25) a heterodimer akin to Rpb4/Rpb7 in Pol II. C17 and C25 were found to interact genetically in suppression screens and physically in coimmunopurification and two-hybrid experiments. Sequence analysis and molecular modeling indicated that the C17/C25 heterodimer likely adopts a structure similar to that of the archaeal RpoE/RpoF counterpart of the Rpb4/Rpb7 complex. These RNA polymerase subunits appear to have evolved to meet the distinct requirements of the multiple forms of RNA polymerases.

  3. Impacts of climate change on streamflows under RCP scenarios: A case study in Xin River Basin, China

    Science.gov (United States)

    Zhang, Yuqing; You, Qinglong; Chen, Changchun; Ge, Jing

    2016-09-01

    Researchers often examine hydro-climatological processes via Global Circulation Model (GCM) and hydrological model, which have been shown to benefit water resources management and prediction, especially at the basin scale. In this study, the Soil and Water Assessment Tool (SWAT) and Statistical Downscaling Method (SDSM) were integrated and applied to estimate streamflows in the Xin River Basin, China, based on climate change scenarios downscaled from different GCMs (BCC-CSM1.1, CanESM2, and NorESM1-M) under three Representative Concentration Pathways (RCPs). Results confirmed that the calibrated SWAT model accurately depicts hydrological processes features at daily, monthly, and yearly scales. Three GCMs based on the calibrated SDSM showed that temperature is continually increasing in the region, however, future precipitation is highly complex and uncertain; there were significant differences among various GCM RCP scenarios. The average of the precipitation in three models showed slight and steady increase trends under RCP2.6 and RCP4.5, but a significant increase under the RCP8.5 scenario. The ensemble average of streamflow in GCMs demonstrated that many RCPs significantly decrease from May to June but increase from August to September relative to the baseline period. The ensemble mean of the multi-GCM indicated that future streamflows under RCP2.6 and RCP4.5 scenarios will be closer to the current streamflow volume. Many RCPs also revealed a significant increase in monthly streamflow dispersion coefficient in October, reflecting a tendency for drought and flood events in that month. The BCC-CSM1.1 and NorESM1-M models showed that streamflows are higher than the baseline with median probability in the future. The low monthly streamflow (10th percentile) processes for each GCM were altogether similar to the baseline, whereas the high monthly streamflows (90th percentile) showed various levels of disparity compared to the baseline.

  4. Winter climate changes over East Asian region under RCP scenarios using East Asian winter monsoon indices

    Science.gov (United States)

    Hong, Ja-Young; Ahn, Joong-Bae; Jhun, Jong-Ghap

    2017-01-01

    The changes in the winter climatology and variability of the East Asian winter monsoon (EAWM) for the late 21st century (2070-2099) under the Representative Concentration Pathway (RCP) 4.5 and 8.5 scenarios are projected in terms of EAWM indices (EAWMIs). Firstly, the capability of the climate models participating in the Coupled Model Intercomparison Project phase 5 (CMIP5) in simulating the boreal winter climatology and the interannual variability of the EAWM for the late 20th century (1971-2000) is examined. Nine of twenty-three climate models are selected based on the pattern correlations with observation and a multi-model ensemble is applied to the nine model data. Three of twelve EAWMIs that show the most significant temporal correlations between the observation and CMIP5 surface air temperatures are utilized. The ensemble CMIP5 is capable of reproducing the overall features of the EAWM in spite of some biases in the region. The negative correlations between the EAWMIs and boreal winter temperature are well reproduced and 3-5 years of the major interannual variation observed in this region are also well simulated according to power spectral analyses of the simulated indices. The fields regressed onto the indices that resemble the composite strong winter monsoon pattern are simulated more or less weakly in CMIP5 compared to the observation. However, the regressed fields of sea level pressure, surface air temperature, 500-hPa geopotential height, and 300-hPa zonal wind are well established with pattern correlations above 0.83 between CMIP5 and observation data. The differences between RCPs and Historical indicate strong warming, which increases with latitude, ranging from 1 to 5 °C under RCP4.5 and from 3 to 7 °C under RCP8.5 in the East Asian region. The anomalous southerly winds generally become stronger, implying weaker EAWMs in both scenarios. These features are also identified with fields regressed onto the indices in RCPs. The future projections reveal

  5. Winter climate changes over East Asian region under RCP scenarios using East Asian winter monsoon indices

    Science.gov (United States)

    Hong, Ja-Young; Ahn, Joong-Bae; Jhun, Jong-Ghap

    2016-03-01

    The changes in the winter climatology and variability of the East Asian winter monsoon (EAWM) for the late 21st century (2070-2099) under the Representative Concentration Pathway (RCP) 4.5 and 8.5 scenarios are projected in terms of EAWM indices (EAWMIs). Firstly, the capability of the climate models participating in the Coupled Model Intercomparison Project phase 5 (CMIP5) in simulating the boreal winter climatology and the interannual variability of the EAWM for the late 20th century (1971-2000) is examined. Nine of twenty-three climate models are selected based on the pattern correlations with observation and a multi-model ensemble is applied to the nine model data. Three of twelve EAWMIs that show the most significant temporal correlations between the observation and CMIP5 surface air temperatures are utilized. The ensemble CMIP5 is capable of reproducing the overall features of the EAWM in spite of some biases in the region. The negative correlations between the EAWMIs and boreal winter temperature are well reproduced and 3-5 years of the major interannual variation observed in this region are also well simulated according to power spectral analyses of the simulated indices. The fields regressed onto the indices that resemble the composite strong winter monsoon pattern are simulated more or less weakly in CMIP5 compared to the observation. However, the regressed fields of sea level pressure, surface air temperature, 500-hPa geopotential height, and 300-hPa zonal wind are well established with pattern correlations above 0.83 between CMIP5 and observation data. The differences between RCPs and Historical indicate strong warming, which increases with latitude, ranging from 1 to 5 °C under RCP4.5 and from 3 to 7 °C under RCP8.5 in the East Asian region. The anomalous southerly winds generally become stronger, implying weaker EAWMs in both scenarios. These features are also identified with fields regressed onto the indices in RCPs. The future projections reveal

  6. Preliminary identification of secreted proteins by Leptospira interrogans serovar Kennewicki strain Pomona Fromm

    Energy Technology Data Exchange (ETDEWEB)

    Ricardi, L.M.P.; Portaro, F.C.; Abreu, P.A.E.; Barbosa, A.S. [Instituto Butantan, Sao Paulo, SP (Brazil); Morais, Z.M.; Vasconcellos, S.A. [Universidade de Sao Paulo (USP), SP (Brazil)

    2012-07-01

    Full text: This project aimed to identify secreted proteins by pathogenic Leptospira interrogans serovar Kennewicki strain Pomona Fromm (LPF) by proteomic analyses. The strain LPF, whose virulence was maintained by passages in hamsters, were cultured in EMJH medium. The supernatants were centrifuged, dialyzed and subjected to lyophilization. Protein samples were resolved first by IEF at pH 3 to 10, immobilized pH gradient 13-cm strips. Strips were then processed for the second-dimension separation on SDS-polyacrylamide gels. Proteins from gel spots were subjected to reduction, cysteine-alkylation, and in-gel tryptic digestion, and analyzed by LC/MS/MS spectrometry. Liquid chromatography-based separation followed by automated tandem mass spectrometry was also used to identify secreted proteins. In silico analyses were performed using the PSORTbV.3.0 program and SignalP server. One major obstacle to secretome studies is the difficulty to obtain extracts of secreted proteins without citoplasmatic contamination. In addition, the extraction of low concentration proteins from large volumes of culture media, which are rich in salts, BSA and other compounds, frequently interfere with most proteomics techniques. For these reasons, several experimental approaches were used to optimize the protocol applied. In spite of this fact, our analysis resulted in the identification of 200 proteins with high confidence. Only 5 of 63 secreted proteins predicted by in silico analysis were found. Other classes identified included proteins that possess signal peptide but whose cellular localization prediction is unknown or may have multiple localization sites, and proteins that lack signal peptide and are thus thought to be secreted via non conventional mechanisms or resulting from cytoplasmic contamination by cell lysis. Many of these are hypothetical proteins with no putative conserved domains detected. To our knowledge, this is the first study to identify secreted proteins by

  7. The RCP Information Laboratory (iLab): breaking the cycle of poor data quality.

    Science.gov (United States)

    Croft, Giles P; Williams, John G

    2005-01-01

    A review of data quality in the NHS by the Audit Commission cited a lack of clinician involvement in the validation and use of centrally held activity data as one of the key issues to resolve. The perception that hospital episode statistics cannot support the needs of the individual clinician results in mistrust and disinterest. This in turn leads to under-development of such data from a clinical perspective, and the cycle continues. The RCP Information Laboratory (iLab) aims to address this problem by accessing, analysing and presenting information from these central repositories concerning the activity of visiting individual consultant physicians. With support from iLab staff--an information analyst and a clinician--local data quality issues are highlighted and local solutions sought. The information obtained can be used as an objective measure of activity to support the processes of appraisal and revalidation.

  8. Future climate data from RCP 4.5 and occurrence of malaria in Korea.

    Science.gov (United States)

    Kwak, Jaewon; Noh, Huiseong; Kim, Soojun; Singh, Vijay P; Hong, Seung Jin; Kim, Duckgil; Lee, Keonhaeng; Kang, Narae; Kim, Hung Soo

    2014-10-15

    Since its reappearance at the Military Demarcation Line in 1993, malaria has been occurring annually in Korea. Malaria is regarded as a third grade nationally notifiable disease susceptible to climate change. The objective of this study is to quantify the effect of climatic factors on the occurrence of malaria in Korea and construct a malaria occurrence model for predicting the future trend of malaria under the influence of climate change. Using data from 2001-2011, the effect of time lag between malaria occurrence and mean temperature, relative humidity and total precipitation was investigated using spectral analysis. Also, a principal component regression model was constructed, considering multicollinearity. Future climate data, generated from RCP 4.5 climate change scenario and CNCM3 climate model, was applied to the constructed regression model to simulate future malaria occurrence and analyze the trend of occurrence. Results show an increase in the occurrence of malaria and the shortening of annual time of occurrence in the future.

  9. Hydrological response to climate change: The Pearl River, China under different RCP scenarios

    Directory of Open Access Journals (Sweden)

    Dan Yan

    2015-09-01

    New hydrological insights for the region: Previous studies focussed on annual discharge and extreme flood events in the basin. However it is also important to assess variations in low flow across the basin, because it is suffering from water shortage and salt water intrusion in the dry season. Results indicate a reduction in average low flow under the five climate models. The reduction varies across the basin and is between 6 and 48% for RCP4.5. River discharge in the dry season is projected to decrease throughout the basin. In the wet season, river discharge tends to increase in the middle and lower reaches and decrease in the upper reach of the Pearl River basin. The variation of river discharge is likely to aggravate water stress. Especially the reduction of low flow is problematic as already now the basin experiences temporary water shortages in the delta.

  10. The Performance Test for Reactor Coolant Pump (RCP) adopting Variable Restriction Orifice Type Control Valve

    Energy Technology Data Exchange (ETDEWEB)

    Kim, S.; Bae, B. U.; Cho, Y. J. and others

    2014-05-15

    The design values of the RCPTF are 17.2 MPa, 343 .deg. C, 11.7 m{sup 3}/s, and 13 MW in the maximum pressure, temperature, flow rate, and electrical power, respectively. In the RCPTF, various types of tests can be performed including a hydraulic performance test to acquire a H-Q curve as well seal transient tests, thrust bearing transient test, cost down test, NPSHR verification test, and so on. After a commissioning startup test was successfully perfomed, mechanical structures are improved including a flow stabilizer and variable restriction orifice. Two- branch pipe (Y-branch) was installed to regulate the flow rate in the range of performance tests. In the main pipe, a flow restrictor (RO: Restriction Orifice) for limiting the maximum flow rate was installed. In the branch pipe line, a globe valve and a butterfly valves for regulating the flow rate was located on the each branch line. When the pressure loss of the valve side is smaller than that of the RO side, the flow rate of valve side was increasing and the flow disturbance was occurred in the lower pipe line. Due to flow disturbnace, it is to cause an error when measuring RCP head and flow measurement of the venturi flow meter installed in the lower main pipe line, and thus leading to a decrease in measurement accuracy as a result. To increase the efficiency of the flow control availability of the test facility, the variable restriction orifice (VRO) type flow control valve was designed and manufactured. In the RCPTF in KAERI, the performance tests and various kinds of transient tests of the RCP were successfully performed. In this study, H-Q curve of the pump using the VRO revealed a similar trend to the result from two ROs. The VRO was confirmed to effectively cover the full test range of the flow rate.

  11. Asymmetry of Surface Climate Change under RCP2.6 Projections from the CMIP5 Models

    Institute of Scientific and Technical Information of China (English)

    XIN Xiaoge; CHENG Yanjie; WANG Fang; WU Tongwen; ZHANG Jie

    2013-01-01

    The multi-model ensemble (MME) of 20 models from the Coupled Model Intercomparison Project Phase Five (CMIP5) was used to analyze surface climate change in the 21st century under the representative concentration pathway RCP2.6,to reflect emission mitigation efforts.The maximum increase of surface air temperature (SAT) is 1.86℃ relative to the pre-industrial level,achieving the target to limit the global warming to 2℃.Associated with the "increase-peak-decline" greenhouse gases (GHGs) concentration pathway of RCP2.6,the global mean SAT of MME shows opposite trends during two time periods:warming during 2006-55 and cooling during 2056-2100.Our results indicate that spatial distribution of the linear trend of SAT during the warming period exhibited asymmetrical features compared to that during the cooling period.The warming during 2006-55 is distributed globally,while the cooling during 2056-2100 mainly occurred in the NH,the South Indian Ocean,and the tropical South Atlantic Ocean.Different dominant roles of heat flux in the two time periods partly explain the asymmetry.During the warming period,the latent heat flux and shortwave radiation both play major roles in heating the surface air.During the cooiing period,the increase of net longwave radiation partly explains the cooling in the tropics and subtropics,which is associated with the decrease of total cloud amount.The decrease of the shortwave radiation accounts for the prominent cooling in the high latitudes of the NH.The surface sensible heat flux,latent heat flux,and shortwave radiation collectively contribute to the especial warming phenomenon in the high-latitude of the SH during the cooling period.

  12. Changes in Temperature Extremes in High Resolution Simulations of RegCM driven by MPI-ESM2 under RCP8.5 and RCP4.5 Scenarios over Turkey

    Science.gov (United States)

    Acar, M.; Ünal, Y. S.

    2015-12-01

    In this study, the effect of climate change on temperature extremes over Turkey is investigated in high resolution climate model simulations driven by a global model outputs. MPI-ESM2 earth system model simulations are downscaled to first 50 km coarse resolution over Med-CORDEX domain and then 10 km high resolution over Turkey by regional climate model, RegCM4.3. The simulations cover the periods of 1970-2000 for the reference and 2015-2100 for the future with proposed changes under Representative Concentration Pathways 4.5 (RCP4.5) and 8.5 (RCP8.5). High resolution gridded surface climate dataset is of great value for the validation phase of the high resolution climate models and the daily temperature observations are interpolated to regular grids which coincide with the simulation's grids by using PRISM (Parameter-elevation Relationships on Independent Slopes Model) approach. High resolution regional climate model performance is evaluated for the reference period by using gridded observations for both averages and extremes of temperature. The RegCM coupled with MPI-ESM2 shows negative biases over most of Turkey. Hence monthly mean value bias correction is applied to temperature simulations of reference and 2015-2100 periods. Extreme temperature climate indices such as FD0, SU25, TX10p, TN10p, TX90p, TN90p, TX35, WSDI, CSDI and DTR are calculated and compared to reference period. Bias corrected high resolution simulations show good agreement with the observations. The climate indices are calculated for the intervals of 2015-2040,2041-2070,2071-2100, for RCP4.5 and RCP8.5 scenarios. FD0, TN10p, TX10p and CSDI indices decrease through the end of century for both scenarios and the most dramatic changes occur on the eastern and central part of Turkey. The rate of decrease is more pronounced in RCP8.5 scenario. On the other hand, the indices of SU25, TX90p, TN90p, TX35 and WSDI increase till 2100. 30 year averages of SU25 and SU35 over all Turkey increase from 64 days

  13. The implications of carbon dioxide and methane exchange for the heavy mitigation RCP2.6 scenario under two metrics

    NARCIS (Netherlands)

    Huntingford, Chris; Lowe, Jason A.; Howarth, Nicholas; Bowerman, Niel H.A.; Gohar, Laila K.; Otto, Alexander; Lee, David S.; Smith, Stephen M.; den Elzen, Michel G.J.; van Vuuren, Detlef P.; Millar, Richard J.; Allen, Myles R.

    2015-01-01

    Greenhouse gas emissions associated with Representative Concentration Pathway RCP2.6 could limit global warming to around or below a 2°C increase since pre-industrial times. However this scenario implies very large and rapid reductions in both carbon dioxide (CO2) and non-CO2 emissions, and suggests

  14. Can the new RCP R0/R1 classification predict the clinical outcome in ductal adenocarcinoma of the pancreatic head?

    Science.gov (United States)

    Janot, M S; Kersting, S; Belyaev, O; Matuschek, A; Chromik, A M; Suelberg, D; Uhl, W; Tannapfel, A; Bergmann, U

    2012-08-01

    According to the International Union Against Cancer (UICC), R1 is defined as the microscopic presence of tumor cells at the surface of the resection margin (RM). In contrast, the Royal College of Pathologists (RCP) suggested to declare R1 already when tumor cells are found within 1 mm of the RM. The aim of this study was to determine the significance of the RM concerning the prognosis of pancreatic ductal adenocarcinoma (PDAC). From 2007 to 2009, 62 patients underwent a curative operation for PDAC of the pancreatic head. The relevance of R status on cumulative overall survival (OS) was assessed on univariate and multivariate analysis for both the classic R classification (UICC) and the suggestion of the RCP. Following the UICC criteria, a positive RM was detected in 8 %. Along with grading and lymph node ratio, R status revealed a significant impact on OS on univariate and multivariate analysis. Applying the suggestion of the RCP, R1 rate rose to 26 % resulting in no significant impact on OS in univariate analysis. Our study has shown that the RCP suggestion for R status has no impact on the prognosis of PDAC. In contrast, our data confirmed the UICC R classification of RM as well as N category, grading, and lymph node ratio as significant prognostic factors.

  15. The implications of carbon dioxide and methane exchange for the heavy mitigation RCP2.6 scenario under two metrics

    NARCIS (Netherlands)

    Huntingford, Chris; Lowe, Jason A.; Howarth, Nicholas; Bowerman, Niel H.A.; Gohar, Laila K.; Otto, Alexander; Lee, David S.; Smith, Stephen M.; den Elzen, Michel G.J.; van Vuuren, Detlef P.; Millar, Richard J.; Allen, Myles R.

    2015-01-01

    Greenhouse gas emissions associated with Representative Concentration Pathway RCP2.6 could limit global warming to around or below a 2°C increase since pre-industrial times. However this scenario implies very large and rapid reductions in both carbon dioxide (CO2) and non-CO2 emissions, and suggests

  16. Isolation of Leptospira interrogans Hardjoprajitno from vaginal fluid of a clinically healthy ewe suggests potential for venereal transmission.

    Science.gov (United States)

    Director, A; Penna, B; Hamond, C; Loureiro, A P; Martins, G; Medeiros, M A; Lilenbaum, W

    2014-09-01

    A total of 15 adult ewes from one flock known to be seroreactive for leptospirosis was studied. Urine and vaginal fluid were collected from each animal to test for the presence of leptospires using bacterial culture and conventional PCR methods. One pure culture of Leptospira sp. was obtained from the vaginal fluid sample of a non-pregnant ewe. The isolate was characterized by DNA sequencing of the rrs and secY genes, variable-number of tandem-repeats (VNTR) analysis and serogrouping, and the isolate was typed as Leptospira interrogans serogroup Sejroe serovar Hardjo type Hardjoprajitno. This report indicates the presence of viable Leptospira in the vaginal fluid of a ewe, suggesting the potential for venereal transmission of leptospires in sheep.

  17. Comparative proteogenomic analysis of the Leptospira interrogans virulence-attenuated strain IPAV against the pathogenic strain 56601

    Institute of Scientific and Technical Information of China (English)

    Yi Zhong; Yuan-Yuan Li; Xiu-Gao Jiang; Guo-Ping Zhao; Shengyue Wang; Yixue Li; Rong Zeng; Xuan Li; Xiao-Kui Guo; Xiao Chang; Xing-Jun Cao; Yan Zhang; Huajun Zheng; Yongzhang Zhu; Chengsong Cai; Zelin Cui; Yunyi Zhang

    2011-01-01

    The virulence-attenuated Leptospira interrogans serovar Lai strain IPAV was derived by prolonged laboratory passage from a highly virulent ancestral strain isolated in China. We studied the genetic variations of IPAV that render it avirulent via comparative analysis against the pathogenic L. interrogans serovar Lai strain 56601. The complete genome sequence of the IPAV strain was determined and used to compare with, and then rectify and reannotate the genome sequence of strain 56601. Aside from their highly similar genomic structure and gene order, a total of 33 insertions, 53 deletions and 301 single-nucleotide variations (SNVs) were detected throughout the genome of IPAV directly affecting 101 genes, either in their 5' upstream region or within their coding region. Among them, the majority of the [44]functional genes are involved in signal transduction, stress response, transmembrane transport and nitrogen metabolism. Comparative proteomic analysis based on quantitative liquid chromatography (LC)-MS/MS data revealed that among 1 627 selected pairs of orthologs, 174 genes in the IPAV strain were upregulated, with enrichment mainly in classes of energy production and lipid metabolism. In contrast, 228 genes in strain 56601 were upregulated, with the majority enriched in the categories of protein translation and DNA replication/repair. The combination of genomic and proteomic approaches illustrated that altered expression or mutations in critical genes, such as those encoding a Ser/Thr kinase, carbon-starvation protein CstA, glutamine synthetase, GTP-binding protein BipA, ribonucleotidediphosphate reductase and phosphate transporter, and alterations in the translational profile of lipoproteins or outer membrane proteins are likely to account for the virulence attenuation in strain IPAV.

  18. The enzyme-linked immunosorbent assay (ELISA) as a serological test for detecting antibodies against Leptospira interrogans serovar hardjo in sheep.

    Science.gov (United States)

    Adler, B; Faine, S; Gordon, L M

    1981-09-01

    The enzyme-liked immunosorbent assay (ELISA) was compared with the standard microscopic agglutination test (MAT) as a method for detecting antibodies against Leptospira interrogans serovar hardjo in sheep. Peak antibody levels detected by the 2 tests occurred at different times following experimental infection of sheep. In serums from flocks of sheep with naturally acquired infection there was a 95% correlation between MAT and ELISA with respect to the presence or absence of antibody to serovar hardjo, although the levels of correlation of the titres of the 2 tests was low. The 2 tests appeared to measure different antigen-antibody systems. The ELISA would be a useful test for screening large numbers of serums for antibodies to L. interrogans serovar hardjo.

  19. Tratamiento del hombro doloroso mediante terapia manual

    OpenAIRE

    Gabucio López, Pedro

    2008-01-01

    Introducción: Las disfunciones de hombro son un problema de salud común en las sociedades occidentales. Algunos protocolos de tratamiento han sido desarrollados mediante ensayos clínicos con pacientes que presentan dolor de hombro. Sin embargo, no hay evidencias que sustenten que un protocolo es mejor que otros. El principal objetivo de este trabajo es presentar un caso clínico en el que mediante terapia manual y la prescripción de ejercicios físicos se consigue la resolución del ...

  20. Tratamiento del hombro doloroso mediante terapia manual

    OpenAIRE

    Gabucio López, Pedro

    2008-01-01

    Introducción: Las disfunciones de hombro son un problema de salud común en las sociedades occidentales. Algunos protocolos de tratamiento han sido desarrollados mediante ensayos clínicos con pacientes que presentan dolor de hombro. Sin embargo, no hay evidencias que sustenten que un protocolo es mejor que otros. El principal objetivo de este trabajo es presentar un caso clínico en el que mediante terapia manual y la prescripción de ejercicios físicos se consigue la resolución del ...

  1. Live imaging of bioluminescent leptospira interrogans in mice reveals renal colonization as a stealth escape from the blood defenses and antibiotics.

    Directory of Open Access Journals (Sweden)

    Gwenn Ratet

    2014-12-01

    Full Text Available Leptospira (L. interrogans are bacteria responsible for a worldwide reemerging zoonosis. Some animals asymptomatically carry L. interrogans in their kidneys and excrete bacteria in their urine, which contaminates the environment. Humans are infected through skin contact with leptospires and develop mild to severe leptospirosis. Previous attempts to construct fluorescent or bioluminescent leptospires, which would permit in vivo visualization and investigation of host defense mechanisms during infection, have been unsuccessful. Using a firefly luciferase cassette and random transposition tools, we constructed bioluminescent chromosomal transformants in saprophytic and pathogenic leptospires. The kinetics of leptospiral dissemination in mice, after intraperitoneal inoculation with a pathogenic transformant, was tracked by bioluminescence using live imaging. For infective doses of 106 to 107 bacteria, we observed dissemination and exponential growth of leptospires in the blood, followed by apparent clearance of bacteria. However, with 2×108 bacteria, the septicemia led to the death of mice within 3 days post-infection. In surviving mice, one week after infection, pathogenic leptospires reemerged only in the kidneys, where they multiplied and reached a steady state, leading to a sustained chronic renal infection. These experiments reveal that a fraction of the leptospiral population escapes the potent blood defense, and colonizes a defined number of niches in the kidneys, proportional to the infective dose. Antibiotic treatments failed to eradicate leptospires that colonized the kidneys, although they were effective against L. interrogans if administered before or early after infection. To conclude, mice infected with bioluminescent L. interrogans proved to be a novel model to study both acute and chronic leptospirosis, and revealed that, in the kidneys, leptospires are protected from antibiotics. These bioluminescent leptospires represent a

  2. [Construction of genomic library of L. interrogans serovar lai using lambda gt11 as the vector and a study of recombiant plasmid pDL121].

    Science.gov (United States)

    Liu, H; Dai, B; Jing, B; Wu, W; Li, S; Fang, Z; Zhao, H; Ye, D; Yan, R; Liu, J; Song, S; Yang, Y; Zhang, Y; Liu, F; Tu, Y; Yang, H; Huang, Z; Liang, L; Hu, L; Zhao, M

    1997-03-01

    A genomic library of L. interrogans serovar lai strain 017 has been constructed using lambda gt11 as the vector. DNA was partially digested by two blunt-end restriction enzymes, then methylated with EcoR I methylase; after EcoR I linker was added to the DNA, the linker-ended DNA was ligated to the dephosphorylated EcoR I digested lambda gt11 arms. The recombined DNA was packaged in vitro, and used to transduct E. coli Y1090 for amplification. There were 2.1 x 10(6) recombinant bacteriophages as recognized by their ability to form white plaques plated on Lac host in the presence of both IPTG and X-Ga1. A positive clone, designated lambda DL12, was screened with a rabbit anti-serum against L. interrogans serovar lai from the genomic library. The DNA from lambda DL12 was subcloned into plasmid pUC18. A recombinant (designated as pDL121) was obtained. SDS-PAGE analysis indicated that a 23 kd was expressed in E. coli JM 103 harboring pDL121. Western blotting analysis showed that a specific protein band molecular weight of 23 kd could be recognized by the rabbit antiserum against L. interrogans serovar lai strain 017.

  3. Future Climate Data from RCP 4.5 and Occurrence of Malaria in Korea

    Directory of Open Access Journals (Sweden)

    Jaewon Kwak

    2014-10-01

    Full Text Available Since its reappearance at the Military Demarcation Line in 1993, malaria has been occurring annually in Korea. Malaria is regarded as a third grade nationally notifiable disease susceptible to climate change. The objective of this study is to quantify the effect of climatic factors on the occurrence of malaria in Korea and construct a malaria occurrence model for predicting the future trend of malaria under the influence of climate change. Using data from 2001–2011, the effect of time lag between malaria occurrence and mean temperature, relative humidity and total precipitation was investigated using spectral analysis. Also, a principal component regression model was constructed, considering multicollinearity. Future climate data, generated from RCP 4.5 climate change scenario and CNCM3 climate model, was applied to the constructed regression model to simulate future malaria occurrence and analyze the trend of occurrence. Results show an increase in the occurrence of malaria and the shortening of annual time of occurrence in the future.

  4. Projected and Observed Aridity and Climate Change in the East Coast of South India under RCP 4.5.

    Science.gov (United States)

    Ramachandran, A; Praveen, Dhanya; Jaganathan, R; Palanivelu, K

    2015-01-01

    In the purview of global warming, the present study attempts to project changes in climate and quantify the changes in aridity of two coastal districts in south India under the RCP 4.5 trajectory. Projected climate change output generated by RegCM 4.4 model, pertaining to 14 grid points located within the study area, was analyzed and processed for this purpose. The meteorological parameters temperature and precipitations were used to create De Martonne Aridity Index, to assess the spatial distribution of aridity. The original index values ranged from 13.7 to 16.4 mm/°C, characterizing this area as a semidry climate. The outcome from the changed scenario analysis under RCP 4.5 showed that, during the end of the 21st century, the aridity may be increased more as the index values tend to reduce. The increasing trend in the drying phenomenon may be attributed to the rising of mean annual temperatures.

  5. ICT based Sustainable Rural Business Opportunities in Developing Countries: A Wireless-Networked RCP-RAP Approach

    Directory of Open Access Journals (Sweden)

    Md. A. Rahman

    2005-01-01

    Full Text Available Information and Communication Technology (ICT is gaining much research attention in present times especially for its ability towards poverty alleviation. In this study the prospect of rural business opportunities in developing countries more specifically in the target country, Bangladesh, has been investigated from the viewpoint of wirelessly networked Rural Communication Point (RCP approach. It is proposed that if only one RCP is set up in every village and there is one Regional Access Point (RAP responsible for several wirelessly networked RCPs, it is recommended that a significant development is possible in rural areas of the target country with the assistance from existing ICT infrastructure. In this study, the target country is chosen as Bangladesh in South Asia but the similar ICT model can be implemented with success in other countries in the developing world.

  6. Stability analysis of a max-min fair Rate Control Protocol (RCP) in a small buffer regime

    CERN Document Server

    Voice, Thomas

    2007-01-01

    In this note we analyse various stability properties of the max-min fair Rate Control Protocol (RCP) operating with small buffers. We first tackle the issue of stability for networks with arbitrary topologies. We prove that the max-min fair RCP fluid model is globally stable in the absence of propagation delays, and also derive a set of conditions for local stability when arbitrary heterogeneous propagation delays are present. The network delay stability result assumes that, at equilibrium, there is only one bottleneck link along each route. Lastly, in the simpler setting of a single link, single delay model, we investigate the impact of the loss of local stability via a Hopf bifurcation.

  7. A probabilistic approach to 21st century regional sea-level projections using RCP and High-end scenarios

    Science.gov (United States)

    Jackson, Luke P.; Jevrejeva, Svetlana

    2016-11-01

    Sea-level change is an integrated climate system response due to changes in radiative forcing, anthropogenic land-water use and land-motion. Projecting sea-level at a global and regional scale requires a subset of projections - one for each sea-level component given a particular climate-change scenario. We construct relative sea-level projections through the 21st century for RCP 4.5, RCP 8.5 and High-end (RCP 8.5 with increased ice-sheet contribution) scenarios by aggregating spatial projections of individual sea-level components in a probabilistic manner. Most of the global oceans adhere to the projected global average sea level change within 5 cm throughout the century for all scenarios; however coastal regions experience localised effects due to the non-uniform spatial patterns of individual components. This can result in local projections that are 10‧s of centimetres different from the global average by 2100. Early in the century, RSL projections are consistent across all scenarios, however from the middle of the century the patterns of RSL for RCP scenarios deviate from the High-end where the contribution from Antarctica dominates. Similarly, the uncertainty in projected sea-level is dominated by an uncertain Antarctic fate. We also explore the effect upon projections of, treating CMIP5 model ensembles as normally distributed when they might not be, correcting CMIP5 model output for internal variability using different polynomials and using different unloading patterns of ice for the Greenland and Antarctic ice sheets.

  8. Changes to extreme wave climates of islands within the Western Tropical Pacific throughout the 21st century under RCP 4.5 and RCP 8.5, with implications for island vulnerability and sustainability

    Science.gov (United States)

    Shope, James B.; Storlazzi, Curt D.; Erikson, Li H.; Hegermiller, Christie A.

    2016-06-01

    Waves are the dominant influence on coastal morphology and ecosystem structure of tropical Pacific islands. Wave heights, periods, and directions for the 21st century were projected using near-surface wind fields from four atmosphere-ocean coupled global climate models (GCM) under representative concentration pathways (RCP) 4.5 and 8.5. GCM-derived wind fields forced the global WAVEWATCH-III wave model to generate hourly time series of bulk wave parameters around 25 islands in the mid to western tropical Pacific Ocean for historical (1976-2005), mid-century, and end-century time periods for the December-February and June-August seasons. The December-February regional wave climate is dominated by strong winds and large swell from extratropical cyclones in the north Pacific while the June-August season brings smaller waves generated by the trade winds and swell from Southern Hemisphere extratropical storms. Extreme significant wave heights decreased ( 10.0%) throughout the 21st century under both climate scenarios compared to historical wave conditions and the higher radiative forcing RCP 8.5 scenario displayed a greater and more widespread decrease in extreme significant wave heights compared to the lower forcing RCP 4.5 scenario. An exception was for the end-century June-August season. Offshore of islands in the central equatorial Pacific, extreme significant wave heights displayed the largest changes from historical values. The frequency of extreme events during December-February decreased under RCP 8.5, whereas the frequency increased under RCP 4.5. Mean wave directions rotated more than 30° clockwise at several locations during June-August, which could indicate a weakening of the trade winds' influence on extreme wave directions and increasing dominance of Southern Ocean swell. The results of this study underscore that December-February large wave events will become smaller and less frequent in most regions, reducing the likelihood and magnitude of wave

  9. Future changes in atmospheric condition for the baiu under RCP scenarios

    Science.gov (United States)

    Okada, Y.; Takemi, T.; Ishikawa, H.

    2015-12-01

    This study focuses on atmospheric circulation fields during the baiu in Japan with global warming projection experimental data conducted using a 20-km mesh global atmospheric model (MRI-AGCM3.2) under Representative Concentration Pathways (RCP) scenarios. This model also used 4 different sea surface temperature (SST) initial conditions. Support of this dataset is provided by the Meteorological Research Institute (MRI). The baiu front indicated by the north-south gradient of moist static energy moves northward in present-day climate, whereas this northward shift in future climate simulations is very slow during May and June. In future late baiu season, the baiu front stays in the northern part of Japan even in August. As a result, the rich water vapor is transported around western Japan and the daily precipitation amount will increase in August. This northward shift of baiu front is associated with the westward expansion of the enhanced the North Pacific subtropical high (NPSH) into Japan region. However, the convective activity around northwest Pacific Ocean is inactive and is unlikely to occur convective jump (CJ). These models show that the weak trough exists in upper troposphere around Japan. Therefore, the cold advection stays in the northern part of Japan during June. In July, the front due to the strengthening of the NPSH moves northward, and then it stays until August. This feature is often found between the clustered SSTs, Cluster 2 and 3. The mean field of future August also show the inflow of rich water vapor content to Japan islands. In this model, the extreme rainfall suggested tends to almost increase over the Japan islands during future summer. This work was conducted under the Program for Risk Information on Climate Change supported by the Ministry of Education, Culture, Sports, Science, and Technology-Japan (MEXT).

  10. Changes of precipitation extremes over South Korea projected by the 5 RCMs under RCP scenarios

    Science.gov (United States)

    Ahn, Joong-Bae; Jo, Sera; Suh, Myoung-Seok; Cha, Dong-Hyun; Lee, Dong-Kyou; Hong, Song-You; Min, Seung-Ki; Park, Seong-Chan; Kang, Hyun-Suk; Shim, Kyo-Moon

    2016-05-01

    The change of extreme precipitation is assessed with the HadGEM2-AO - 5 Regional Climate Models (RCMs) chain, which is a national downscaling project undertaken cooperatively by several South Korean institutes aimed at producing regional climate change projection with fine resolution (12.5 km) around the Korean Peninsula. The downscaling domain, resolution and lateral boundary conditions are held the same among the 5 RCMs to minimize the uncertainties from model configuration. Climatological changes reveal a statistically significant increase in the mid-21st century (2046- 2070; Fut1) and the late-21st century (2076-2100; Fut2) precipitation properties related to extreme precipitation, such as precipitation intensity and average of upper 5 percentile daily precipitation, with respect to the reference period (1981-2005). Changes depending on the intensity categories also present a clear trend of decreasing light rain and increasing heavy rain. In accordance with these results, the change of 1-in-50 year maximum precipitation intensity over South Korea is estimated by the GEV method. The result suggests that the 50-year return value (RV50) will change from -32.69% to 72.7% and from -31.6% to 96.32% in Fut1 and from -31.97% to 86.25% and from -19.45% to 134.88% in Fut2 under representative concentration pathway (RCP) 4.5 and 8.5 scenarios, respectively, at the 90% confidence level. This study suggests that multi-RCMs can be used to reduce uncertainties and assess the future change of extreme precipitation more reliably. Moreover, future projection of the regional climate change contains uncertainties evoked from not only driving GCM but also RCM. Therefore, multi-GCM and multi-RCM studies are expected to provide more robust projection.

  11. Features of Two New Proteins with OmpA-Like Domains Identified in the Genome Sequences of Leptospira interrogans

    Science.gov (United States)

    Teixeira, Aline F.; de Morais, Zenaide M.; Kirchgatter, Karin; Romero, Eliete C.; Vasconcellos, Silvio A.; Nascimento, Ana Lucia T. O.

    2015-01-01

    Leptospirosis is an acute febrile disease caused by pathogenic spirochetes of the genus Leptospira. It is considered an important re-emerging infectious disease that affects humans worldwide. The knowledge about the mechanisms by which pathogenic leptospires invade and colonize the host remains limited since very few virulence factors contributing to the pathogenesis of the disease have been identified. Here, we report the identification and characterization of two new leptospiral proteins with OmpA-like domains. The recombinant proteins, which exhibit extracellular matrix-binding properties, are called Lsa46 - LIC13479 and Lsa77 - LIC10050 (Leptospiral surface adhesins of 46 and 77 kDa, respectively). Attachment of Lsa46 and Lsa77 to laminin was specific, dose dependent and saturable, with KD values of 24.3 ± 17.0 and 53.0 ± 17.5 nM, respectively. Lsa46 and Lsa77 also bind plasma fibronectin, and both adhesins are plasminogen (PLG)-interacting proteins, capable of generating plasmin (PLA) and as such, increase the proteolytic ability of leptospires. The proteins corresponding to Lsa46 and Lsa77 are present in virulent L. interrogans L1-130 and in saprophyte L. biflexa Patoc 1 strains, as detected by immunofluorescence. The adhesins are recognized by human leptospirosis serum samples at the onset and convalescent phases of the disease, suggesting that they are expressed during infection. Taken together, our data could offer valuable information to the understanding of leptospiral pathogenesis. PMID:25849456

  12. Features of two new proteins with OmpA-like domains identified in the genome sequences of Leptospira interrogans.

    Directory of Open Access Journals (Sweden)

    Aline F Teixeira

    Full Text Available Leptospirosis is an acute febrile disease caused by pathogenic spirochetes of the genus Leptospira. It is considered an important re-emerging infectious disease that affects humans worldwide. The knowledge about the mechanisms by which pathogenic leptospires invade and colonize the host remains limited since very few virulence factors contributing to the pathogenesis of the disease have been identified. Here, we report the identification and characterization of two new leptospiral proteins with OmpA-like domains. The recombinant proteins, which exhibit extracellular matrix-binding properties, are called Lsa46 - LIC13479 and Lsa77 - LIC10050 (Leptospiral surface adhesins of 46 and 77 kDa, respectively. Attachment of Lsa46 and Lsa77 to laminin was specific, dose dependent and saturable, with KD values of 24.3 ± 17.0 and 53.0 ± 17.5 nM, respectively. Lsa46 and Lsa77 also bind plasma fibronectin, and both adhesins are plasminogen (PLG-interacting proteins, capable of generating plasmin (PLA and as such, increase the proteolytic ability of leptospires. The proteins corresponding to Lsa46 and Lsa77 are present in virulent L. interrogans L1-130 and in saprophyte L. biflexa Patoc 1 strains, as detected by immunofluorescence. The adhesins are recognized by human leptospirosis serum samples at the onset and convalescent phases of the disease, suggesting that they are expressed during infection. Taken together, our data could offer valuable information to the understanding of leptospiral pathogenesis.

  13. Occurrence of antibodies anti -Toxoplasma gondii, Neospora caninum and Leptospira interrogans in a captive deer herd in Southern Brazil

    Directory of Open Access Journals (Sweden)

    Cristina Kraemer Zimpel

    Full Text Available Abstract A large number of Brazilian zoos keep many endangered species of deer, however, very few disease surveillance studies have been conducted among captive cervids. Blood samples from 32 Brazilian deer (Blastocerus dichotomus, Mazama nana and Mazama americana kept in captivity at Bela Vista Biological Sanctuary (Foz do Iguaçu, Brazil were investigated for 10 ruminant pathogens, with the aims of monitoring deer health status and evaluating any potential zoonotic risk. Deer serum samples were tested for Brucella abortus, Leptospira (23 serovars, Toxoplasma gondii, Neospora caninum, bovine viral diarrhea virus, infectious bovine rhinotracheitis virus, foot-and-mouth disease virus, western equine encephalitis virus, eastern equine encephalitis virus and Venezuelan equine encephalitis virus. Antibodies against T. gondii (15.6%, N. caninum (6.2% and L. interrogans serogroup Serjoe (3.1% were detected. The serological results for all other infectious agents were negative. The deer were considered to be clinically healthy and asymptomatic regarding any disease. Compared with studies on free-ranging deer, the prevalences of the same agents tested among the captive deer kept at the Sanctuary were lower, thus indicating good sanitary conditions and high-quality management practices at the zoo.

  14. Occurrence of antibodies anti -Toxoplasma gondii, Neospora caninum and Leptospira interrogans in a captive deer herd in Southern Brazil.

    Science.gov (United States)

    Zimpel, Cristina Kraemer; Grazziotin, Ana Laura; de Barros Filho, Ivan Roque; Guimaraes, Ana Marcia de Sa; dos Santos, Leonilda Correia; de Moraes, Wanderlei; Cubas, Zalmir Silvino; de Oliveira, Marcos Jose; Pituco, Edviges Maristela; Lara, Maria do Carmo Custódio de Souza Hunold; Villalobos, Eliana Monteforte Cassaro; Silva, Lília Marcia Paulin; Cunha, Elenice Maria Sequetin; Castro, Vanessa; Biondo, Alexander Welker

    2015-01-01

    A large number of Brazilian zoos keep many endangered species of deer, however, very few disease surveillance studies have been conducted among captive cervids. Blood samples from 32 Brazilian deer (Blastocerus dichotomus, Mazama nana and Mazama americana) kept in captivity at Bela Vista Biological Sanctuary (Foz do Iguaçu, Brazil) were investigated for 10 ruminant pathogens, with the aims of monitoring deer health status and evaluating any potential zoonotic risk. Deer serum samples were tested for Brucella abortus, Leptospira (23 serovars), Toxoplasma gondii, Neospora caninum, bovine viral diarrhea virus, infectious bovine rhinotracheitis virus, foot-and-mouth disease virus, western equine encephalitis virus, eastern equine encephalitis virus and Venezuelan equine encephalitis virus. Antibodies against T. gondii (15.6%), N. caninum (6.2%) and L. interrogans serogroup Serjoe (3.1%) were detected. The serological results for all other infectious agents were negative. The deer were considered to be clinically healthy and asymptomatic regarding any disease. Compared with studies on free-ranging deer, the prevalences of the same agents tested among the captive deer kept at the Sanctuary were lower, thus indicating good sanitary conditions and high-quality management practices at the zoo.

  15. Cytotoxic activity and probable apoptotic effect of Sph2, a sphigomyelinase hemolysin from Leptospira interrogans strain Lai.

    Science.gov (United States)

    Zhang, Yi-Xuan; Geng, Yan; Yang, Jun-Wei; Guo, Xiao-Kui; Zhao, Guo-Ping

    2008-02-29

    Our previous work confirmed that Sph2/LA1029 was a sphigomyelinase-like hemolyisn of Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai. Characteristics of both hemolytic and cytotoxic activities of Sph2 were reported in this paper. Sph2 was a heat-labile neutral hemolysin and had similar hemolytic behavior as the typical sphingomyelinase C of Staphylococcus aureus upon sheep erythrocytes. The cytotoxic activity of Sph2 was shown in mammalian cells such as BALB/C mouse lymphocytes and macrophages, as well as human L-02 liver cells. Transmission electron microscopic observation showed that the Sph2 treated BALB/C mouse lymphocytes were swollen and ruptured with membrane breakage. They also demonstrated condensed chromatin as a high-density area. Cytoskeleton changes were observed via fluorescence confocal microscope in Sph2 treated BALB/C mouse lymphocytes and macrophages, where both cytokine IL-1beta and IL-6 were induced. In addition, typical apoptotic morphological features were observed in Sph2 treated L-02 cells via transmission electron microscope and the percentage of apoptotic cells did increase after the Sph2 treatment detected by flow cytometry. Therefore, Sph2 was likely an apoptosis-inducing factor of human L-02 liver cells.

  16. Changes to extreme wave climates of islands within the Western Tropical Pacific throughout the 21st century under RCP 4.5 and RCP 8.5, with implications for island vulnerability and sustainability

    Science.gov (United States)

    Shope, James B.; Storlazzi, Curt; Erikson, Li; Hegermiller, Christie

    2016-01-01

    Waves are the dominant influence on coastal morphology and ecosystem structure of tropical Pacific islands. Wave heights, periods, and directions for the 21st century were projected using near-surface wind fields from four atmosphere-ocean coupled global climate models (GCM) under representative concentration pathways (RCP) 4.5 and 8.5. GCM-derived wind fields forced the global WAVEWATCH-III wave model to generate hourly time-series of bulk wave parameters around 25 islands in the mid to western tropical Pacific Ocean for historical (1976–2005), mid-, and end-of-century time periods. Extreme significant wave heights decreased (~10.0%) throughout the 21st century under both climate scenarios compared to historical wave conditions and the higher radiative forcing 8.5 scenario displayed a greater and more widespread decrease in extreme significant wave heights compared to the lower forcing 4.5 scenario. An exception was for the end-of-century June–August season. Offshore of islands in the central equatorial Pacific, extreme significant wave heights displayed the largest changes from historical values. The frequency of extreme events during December–February decreased under RCP 8.5, whereas the frequency increased under RCP 4.5. Mean wave directions often rotated more than 30° clockwise at several locations during June–August, which could indicate a weakening of the trade winds’ influence on extreme wave directions and increasing dominance of Southern Ocean swell or eastern shift of storm tracks. The projected changes in extreme wave heights, directions of extreme events, and frequencies at which extreme events occur will likely result in changes to the morphology and sustainability of island nations.

  17. [Nucleotide sequence analysis of a species specific probe by an inserted fragment from recombinant plasmid pCX7 of L. interrogans sensu stricto serovar lai].

    Science.gov (United States)

    Dai, B; Xiao, J; Yan, Z; Shen, C; Li, S; Fang, Z

    1998-12-01

    The etiological agents of leptospirosis are the pathogenic leptospires (L. interrogans sensu lato) which can be divided into 223 serovars organized into 23 serogroups. The serovar remains the basic taxon, but serotyping may now be accomplished and recognized by acceptable methods. Complementary molecular approaches are being used extensively to assess genetic relatedness amongst leptospires with restriction endonuclese analysis (REA), pulse field gel electrophoresis (PFGE) and DNA-DNA hybridization as well established tools. However, the method is cumbersome and unsuitable for routine application. To develop a sensitive and specific method for identification of pathogenic leptospires, a genomic library of L. interrogans sensu stricto serovar lai was constructed with the plasmid vector pUC9. A recombinant plasmid, designated pCX7 which has homologous fragment of pathogenic leptospires was screened from the bank. pCX7 could recognize pathogenic leptospiral DNA fragment 1.7 kb of strain 017 without cross hybridization to nonpathogenic leptospiral DNA. Inserted fragment of pCX7 DNA sequencing was performed by Dr. Yan Zhengxin (Max-Plank-Institut fur Biology, Tubingen, Germany). Insert fragment was cloned into pBluescript and sequenced by using ABI(Applied Bio. Systems, Model 373A). Nucleotide sequences were analyzed by Dr. Xiao Jianguo (Texas University Medical School and School of Public Health, Center for Infectious Diseases) using a suit of computer program (NIH). One open reading frame of 306 nucleotids were identified. There were identifiable initiation codons, terminators, pribnow box and sextama box within the sequenced regions. These results further confirmed that the little homology between L. interrogans sensu strito and L. borgpeterseni serovar javanica, L. inadai serovar ranarun and serovar manhao (L. genomospecies 2), L. biflexa serovar patoc, L. illini. pCX7 DNA probe could provide a base for identification and classification of leptospires.

  18. Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil Isolamento e caracterização de Leptospira interrogans de suínos abatidos no Estado de São Paulo, Brasil

    Directory of Open Access Journals (Sweden)

    Fabiana Miraglia

    2008-09-01

    Full Text Available With the aim of isolating Leptospira spp., blood serum, kidney, liver and genital tract of 137 female swine (40 sows and 97 gilts and also urine samples from 22 sows were collected in a slaughterhouse in the State of São Paulo, from April 2003 to August 2004. Four isolates were obtained from animals that presented microagglutination test (MAT titers > 100 for the serovar Pomona and one was obtained from an animal negative by MAT in which Leptospira was isolated from the liver and reproductive tract. The presence of leptospiral DNA was investigated by PCR, and positive results were found in kidneys of 11 females, liver of two, genital tract of two and urine of one of them. Nephrosis, interstitial multifocal nephritis, moderate to severe changing, hyalines cylinders and hemorrhagic focuses, hepatic and uterine horns congestion were histological lesions observed in higher frequency in animals positive for leptospira. The silver impregnation (Warthin Starry confirmed the presence of spirochetes in renal tubules of four females with positive leptospira cultures from kidneys. The serogroup of the five isolates was identified as Pomona by cross agglutination with reference polyclonal antibodies. Molecular characterization of the isolates was carried out by variable-number tandem-repeats analysis. All the isolates revealed a pattern distinct from the L. interrogans Pomona type strain, but identical to a previously identified pattern from strains isolated in Argentina belonging to serovar Pomona.Amostras de soro sanguíneo, rim, fígado e trato genital de 137 fêmeas suínas (40 matrizes e 97 marrãs e de urina de 22 matrizes foram colhidas em abatedouro no Estado de São Paulo, no período de abril de 2003 a agosto de 2004 tendo como objetivo o isolamento de Leptospira spp. Quatro estirpes foram isoladas de animais que apresentaram títulos, no teste de soroaglutinação microscópica (SAM > 100, para o sorovar Pomona e de um animal, não reagente na

  19. The Rab11-FIP1/RCP gene codes for multiple protein transcripts related to the plasma membrane recycling system.

    Science.gov (United States)

    Jin, Min; Goldenring, James R

    2006-06-01

    Rab11a is a member of the Rab11 small GTPase family, and plays an important role in plasma membrane recycling. Rab11-Family Interacting Protein 1 (Rab11-FIP1) binds to Rab11 through a carboxyl-terminal amphipathic alpha helix. We have identified eight alternatively spliced Rab11-FIP1 gene transcripts from human chromosome 8. Among them, Rab11-FIP1A-D have carboxyl terminal Rab11 binding domains, while Rab11-FIP1E-H do not contain the Rab11 binding domain. While Rab11-FIP1B and F gene transcripts are ubiquitous, other Rab11-FIP1 transcripts demonstrate more limited patterns of expression in human tissue cDNAs. EGFP-Rab11-FIP1A-D proteins over-expressed in HeLa cells targeted to Rab11a-containing membranes, while EGFP-Rab11-FIP1E/F and H proteins did not localize with recycling system membranes. However, transferrin trafficking was not significantly altered in HeLa cells over-expressing expressing any of the EGFP-Rab11-FIP1 proteins. Rabbit polyclonal antibodies specific for Rab11-FIP1B and Rab11-FIP1C/RCP demonstrated that Rab11-FIP1B and Rab11-FIP1C/RCP are expressed endogenously. Strikingly, endogenous staining for Rab11-FIP1C/RCP only partially co-localized with EGFP-Rab11-FIP1A, EGFP-Rab11-FIP1B, and EGFP-Rab11a in the perinuclear region, indicating that Rab11-FIP1C/RCP resides in a differentiable subcellular compartment within the plasma membrane recycling system compared with Rab11-FIP1A and Rab11-FIP1B. These data suggest that Rab11-FIP1 proteins may play coordinated roles in regulating plasma membrane recycling with regional specificity within the Rab11a-containing recycling system.

  20. Identification of Legionella pneumophila rcp, a pagP-like gene that confers resistance to cationic antimicrobial peptides and promotes intracellular infection.

    Science.gov (United States)

    Robey, M; O'Connell, W; Cianciotto, N P

    2001-07-01

    In the course of characterizing a locus involved in heme utilization, we identified a Legionella pneumophila gene predicted to encode a protein with homology to the product of the Salmonella enterica serovar Typhimurium pagP gene. In Salmonella, pagP increases resistance to the bactericidal effects of cationic antimicrobial peptides (CAMPs). Mutants with insertions in the L. pneumophila pagP-like gene were generated and showed decreased resistance to different structural classes of CAMPs compared to the wild type; hence, this gene was designated rcp for resistance to cationic antimicrobial peptides. Furthermore, Legionella CAMP resistance was induced by growth in low-magnesium medium. To determine whether rcp had any role in intracellular survival, mutants were tested in the two most relevant host cells for Legionnaires' disease, i.e., amoebae and macrophages. These mutants exhibited a 1,000-fold-decreased recovery during a Hartmannella vermiformis coculture. Complementation of the infectivity defect could be achieved by introduction of a plasmid containing the intact rcp gene. Mutations in rcp consistently reduced both the numbers of bacteria recovered during intracellular infection and their cytopathic capacity for U937 macrophages. The rcp mutant was also more defective for lung colonization of A/J mice. Growth of rcp mutants in buffered yeast extract broth was identical to that of the wild type, indicating that the observed differences in numbers of bacteria recovered from host cells were not due to a generalized growth defect. However, in low-Mg(2+) medium, the rcp mutant was impaired in stationary-phase survival. This is the first demonstration of a pagP-like gene, involved in resistance to CAMPs, being required for intracellular infection and virulence.

  1. Applying IPCC Representative Concentration Pathway (RCP) land-use projections in a regional assessment of land-use change in the conterminous United States.

    Science.gov (United States)

    Sherba, J.; Sleeter, B. M.

    2015-12-01

    The Intergovernmental Panel on Climate Change (IPCC) Representative Concentration Pathways (RCPs) include global land-use change projections for four global emissions scenarios. These projections are potentially useful for driving regional-scale models needed for informing land-use and management interactions. Here, we applied global gridded RCP land-use projections within a regional-scale state-and-transition simulation model (STSM) projecting land-use change in the conterminous United States. First, we cross-walked RCP land-use transition classes to land-use classes more relevant for modeling at the regional scale. Coarse grid RCP land-use transition values were then downscaled to EPA Level III ecoregion boundaries using historical land-use transition data from the USGS Land Cover Trends (LCT) dataset. Downscaled transitions were aggregated to the ecoregion level. Ecoregions were chosen because they represent areas with consistent land-use patterns that have proven useful for studying land-use and management interactions. Ecoregion-level RCP projections were applied in a state-and-transition simulation model (STSM) projecting land-use change between 2005 and 2100 at the 1-km scale. Resulting RCP-based STSM projections were compared to STSM projections created using scenario projections from the Special Report on Emissions Scenarios (SRES) and the USGS LCT dataset. While most land-use trajectories appear plausible, some transitions such as forest harvest are unreasonable in the context of historical land-use patterns and the socio-economic drivers of change outlined for each scenario. This effort provides a method for using the RCP land-use projections in a wide range of regional scale models. However, further investigation is needed into the performance of RCP land-use projections at the regional scale.

  2. Prozone effects in microscopic agglutination tests for leptospirosis in the sera of mice infected with the pathogenic Leptospira interrogans serovar Canicola

    Directory of Open Access Journals (Sweden)

    Fabio Hiroto Shimabukuro

    2013-08-01

    Full Text Available Mice experimentally infected with a pathogenic strain of Leptospira interrogans serovar Canicola produced false negative results (prozone effect in a microscopic agglutination test (MAT. This prozone effect occurred in several serum samples collected at different post-infection times, but it was more prominent in samples collected from seven-42 days post-infection and for 1:50 and 1:100 sample dilutions. This phenomenon was correlated with increased antibody titres in the early post-infection phase. While prozone effects are often observed in serological agglutination assays for the diagnosis of animal brucellosis and human syphilis, they are not widely reported in leptospirosis MATs.

  3. Isolation of Salmonella enterica and serologic reactivity to Leptospira interrogans in opossums (Didelphis virginiana from Yucatán, México Aislamiento de Salmonella enterica y reactividad serológica a Leptospira interrogans en tlacuaches (Didelphis virginiana de Yucatán, México

    Directory of Open Access Journals (Sweden)

    Hugo Antonio RUIZ-PIÑA

    2002-07-01

    Full Text Available The presence of Salmonella enterica and serologic evidence of infection by Leptospira interrogans, were detected in the opossum Didelphis virginiana in a semi-urban locality of the Yucatán State, México. Ninety-one opossums were captured during the period April 1996 and May 1998. From a total of 17 feces samples, four Salmonella enterica subsp. enterica serotypes (Sandiego, Newport, Anatum, and Minnesota, and one Salmonella enterica subsp. arizonae serovar O44:Z4,Z23:- were isolated. Some opossums presented mixed infections. From 81 sera samples, four (4.9% were positive to antibodies to Leptospira serovars pomona and wolfii. Both animals infected with Salmonella enterica and those serologically positive to Leptospira interrogans were captured in peridomestic habitat. Opossums infected with Salmonella enterica, were captured in dry season, and those seropositive to Leptospira interrogans during the rainy season. The implications of infection and reactivity of these zoonotic pathogens in D. virginiana in the Yucatan state are briefly discussed.La presencia de Salmonella enterica y evidencia serológica de infección por Leptospira interrogans fueron detectadas en tlacuaches de la especie Didelphis virginiana capturados en una localidad semi-urbana del estado de Yucatán, México. Se capturaron 91 marsupiales durante el período de abril de 1996 a mayo de 1998. De un total de 17 muestras de heces, se aislaron cuatro serotipos de Salmonella enterica subsp. enterica (Sandiego, Newport, Anatum y Minnesota y una Salmonella enterica subsp. arizonae serovar O44:Z4,Z23:-. En algunos tlacuaches se registraron infecciones mixtas. De 81 muestras de suero, cuatro (4,9% presentaron reacciones positivas con los serovares pomona y wolffi, ambos pertenecientes al género Leptospira. Los tlacuaches con serología positiva fueron capturados en el hábitat peridomiciliar. Los animales infectados con Salmonella enterica fueron capturados en los períodos de seca y

  4. Leptospira interrogans activation of peripheral blood monocyte glycolipoprotein demonstrated in whole blood by the release of IL-6

    Directory of Open Access Journals (Sweden)

    F. Dorigatti

    2005-06-01

    Full Text Available Glycolipoprotein (GLP from pathogenic serovars of Leptospira has been implicated in the pathogenesis of leptospirosis by its presence in tissues of experimental animals with leptospirosis, the inhibition of the Na,K-ATPase pump activity, and induced production of cytokines. The aims of the present study were to investigate the induction of IL-6 by GLP in peripheral blood mononuclear cells (PBMC and to demonstrate monocyte stimulation at the cellular level in whole blood from healthy volunteers. PBMC were stimulated with increasing concentrations (5 to 2500 ng/ml of GLP extracted from the pathogenic L. interrogans serovar Copenhageni, lipopolysaccharide (positive control or medium (negative control, and supernatants were collected after 6, 20/24, and 48 h, and kept at -80ºC until use. Whole blood was diluted 1:1 in RPMI medium and cultivated for 6 h, with medium, GLP and lipopolysaccharide as described above. Monensin was added after the first hour of culture. Supernatant cytokine levels from PBMC were measured by ELISA and intracellular IL-6 was detected in monocytes in whole blood cultures by flow-cytometry. Monocytes were identified in whole blood on the basis of forward versus side scatter parameters and positive reactions with CD45 and CD14 antibodies. GLP ( > or = 50 ng/ml-induced IL-6 levels in supernatants were detected after 6-h incubation, reaching a peak after 20/24 h. The percentage of monocytes staining for IL-6 increased with increasing GLP concentration. Thus, our findings show a GLP-induced cellular activation by demonstrating the ability of GLP to induce IL-6 and the occurrence of monocyte activation in whole blood at the cellular level.

  5. A comprehensive approach to identification of surface-exposed, outer membrane-spanning proteins of Leptospira interrogans.

    Directory of Open Access Journals (Sweden)

    Marija Pinne

    Full Text Available Leptospirosis is a zoonosis with worldwide distribution caused by pathogenic spirochetes belonging to the genus Leptospira. The leptospiral life cycle involves transmission via fresh water and colonization of the renal tubules of their reservoir hosts or infection of accidental hosts, including humans. Bacterial outer membrane proteins (OMPs, particularly those with surface-exposed regions, play crucial roles in virulence mechanisms of pathogens and the adaptation to various environmental conditions, including those of the mammalian host. Little is known about the surface-exposed OMPs in Leptospira, particularly those with outer membrane-spanning domains. Herein, we describe a comprehensive strategy for identification and characterization of leptospiral transmembrane OMPs. The genomic sequence of L. interrogans serovar Copenhageni strain Fiocruz L1-130 allowed us to employ the beta-barrel prediction programs, PRED-TMBB and TMBETA-NET, to identify potential transmembrane OMPs. Several complementary methods were used to characterize four novel OMPs, designated OmpL36, OmpL37, OmpL47 and OmpL54. In addition to surface immunofluorescence and surface biotinylation, we describe surface proteolysis of intact leptospires as an improved method for determining the surface exposure of leptospiral proteins. Membrane integration was confirmed using techniques for removal of peripheral membrane proteins. We also demonstrate deficiencies in the Triton X-114 fractionation method for assessing the outer membrane localization of transmembrane OMPs. Our results establish a broadly applicable strategy for the elucidation of novel surface-exposed outer membrane-spanning proteins of Leptospira, an essential step in the discovery of potential virulence factors, diagnostic antigens and vaccine candidates.

  6. Cytotoxic activities of Leptospira interrogans hemolysin SphH as a pore-forming protein on mammalian cells.

    Science.gov (United States)

    Lee, Seoung Hoon; Kim, Sangduk; Park, Seung Chul; Kim, Min Ja

    2002-01-01

    Leptospirosis is a spirochetal zoonosis that causes an acute febrile systemic illness in humans. Leptospira sp. hemolysins have been shown to be virulence factors for the pathogenesis of leptospirosis. Previously, we cloned a hemolysin SphH of Leptospira interrogans serovar lai, a homologue of L. borgpetersenii sphingomyelinase (SphA), from a genomic library (S. H. Lee, K. A. Kim, Y. K. Kim, I. W. Seong, M. J. Kim, and Y. J. Lee, Gene 254:19-28, 2000). Escherichia coli lysate harboring the sphH showed high hemolytic activities on sheep erythrocytes. However, it neither showed sphingomyelinase nor phospholipase activities, in contrast to SphA which was known to have sphingomyelinase activity. Interestingly, the SphH-mediated hemolysis on erythrocytes was osmotically protected by PEG 5000, suggesting that the SphH might have caused pore formation on the erythrocyte membrane. In the present study, we have prepared the Leptospira hemolysin SphH and investigated its hemolytic and cytotoxic activities on mammalian cells. SphH was shown to be a pore-forming protein on several mammalian cells: When treated with the SphH, the sheep erythrocyte membranes formed pores, which were morphologically confirmed by transmission electron microscopy. Furthermore, the SphH-mediated cytotoxicities on mammalian cells were demonstrated by the release of LDH and by inverted microscopic examinations. Finally, the immune serum against the full-length hemolysin could effectively neutralize the SphH-mediated hemolytic and cytotoxic activities. In conclusion, these results suggest that the virulence of Leptospira SphH was due to the pore formation on mammalian cell membranes.

  7. Identification of seroreactive proteins of Leptospira interrogans serovar copenhageni using a high-density protein microarray approach.

    Science.gov (United States)

    Lessa-Aquino, Carolina; Borges Rodrigues, Camila; Pablo, Jozelyn; Sasaki, Rie; Jasinskas, Algis; Liang, Li; Wunder, Elsio A; Ribeiro, Guilherme S; Vigil, Adam; Galler, Ricardo; Molina, Douglas; Liang, Xiaowu; Reis, Mitermayer G; Ko, Albert I; Medeiros, Marco Alberto; Felgner, Philip L

    2013-01-01

    Leptospirosis is a widespread zoonotic disease worldwide. The lack of an adequate laboratory test is a major barrier for diagnosis, especially during the early stages of illness, when antibiotic therapy is most effective. Therefore, there is a critical need for an efficient diagnostic test for this life threatening disease. In order to identify new targets that could be used as diagnostic makers for leptopirosis, we constructed a protein microarray chip comprising 61% of Leptospira interrogans proteome and investigated the IgG response from 274 individuals, including 80 acute-phase, 80 convalescent-phase patients and 114 healthy control subjects from regions with endemic, high endemic, and no endemic transmission of leptospirosis. A nitrocellulose line blot assay was performed to validate the accuracy of the protein microarray results. We found 16 antigens that can discriminate between acute cases and healthy individuals from a region with high endemic transmission of leptospirosis, and 18 antigens that distinguish convalescent cases. Some of the antigens identified in this study, such as LipL32, the non-identical domains of the Lig proteins, GroEL, and Loa22 are already known to be recognized by sera from human patients, thus serving as proof-of-concept for the serodiagnostic antigen discovery approach. Several novel antigens were identified, including the hypothetical protein LIC10215 which showed good sensitivity and specificity rates for both acute- and convalescent-phase patients. Our study is the first large-scale evaluation of immunodominant antigens associated with naturally acquired leptospiral infection, and novel as well as known serodiagnostic leptospiral antigens that are recognized by antibodies in the sera of leptospirosis cases were identified. The novel antigens identified here may have potential use in both the development of new tests and the improvement of currently available assays for diagnosing this neglected tropical disease. Further

  8. Identification of seroreactive proteins of Leptospira interrogans serovar copenhageni using a high-density protein microarray approach.

    Directory of Open Access Journals (Sweden)

    Carolina Lessa-Aquino

    Full Text Available Leptospirosis is a widespread zoonotic disease worldwide. The lack of an adequate laboratory test is a major barrier for diagnosis, especially during the early stages of illness, when antibiotic therapy is most effective. Therefore, there is a critical need for an efficient diagnostic test for this life threatening disease.In order to identify new targets that could be used as diagnostic makers for leptopirosis, we constructed a protein microarray chip comprising 61% of Leptospira interrogans proteome and investigated the IgG response from 274 individuals, including 80 acute-phase, 80 convalescent-phase patients and 114 healthy control subjects from regions with endemic, high endemic, and no endemic transmission of leptospirosis. A nitrocellulose line blot assay was performed to validate the accuracy of the protein microarray results.We found 16 antigens that can discriminate between acute cases and healthy individuals from a region with high endemic transmission of leptospirosis, and 18 antigens that distinguish convalescent cases. Some of the antigens identified in this study, such as LipL32, the non-identical domains of the Lig proteins, GroEL, and Loa22 are already known to be recognized by sera from human patients, thus serving as proof-of-concept for the serodiagnostic antigen discovery approach. Several novel antigens were identified, including the hypothetical protein LIC10215 which showed good sensitivity and specificity rates for both acute- and convalescent-phase patients.Our study is the first large-scale evaluation of immunodominant antigens associated with naturally acquired leptospiral infection, and novel as well as known serodiagnostic leptospiral antigens that are recognized by antibodies in the sera of leptospirosis cases were identified. The novel antigens identified here may have potential use in both the development of new tests and the improvement of currently available assays for diagnosing this neglected tropical disease

  9. Investigation on the presence of leptospires in ovaries of hamsters experimentally infected whith Leptospiras interrogans serovar pomona

    Directory of Open Access Journals (Sweden)

    Claudio Roberto de Almeida Camargo

    1993-12-01

    Full Text Available After inoculating L. interrogans serovar pomona in 75 primiparous hamsters (Mesocricetus auratus, the invasiveness of leptospires into lhe ovaries and lhe ability in causing ovary morphologic alterations were investigated by means of microscopic examination and bacterial isolation. For this purpose, 75 hamsters were inoculated with 0.5 ml of virulent strain containing 30-40 leptospires by the microscopic field and the other 15 hamsters were held as the uninfected controls. Signs and symptoms (prostration, tachypnea, rufled hair, jaundice, and nasal, bucal and perineal hemorrage were detected in all inoculated animals. The animals were killed in the agonic state of the illness, which were done through 4th and 7th day post inoculation. The ovaries were taken asseptically during the necropsies, thoroughly washed using the sterile phosphate buffered saline, in order to eliminate the possible external contamination. The fresh ovary samples were submitted to the dark field direct microscopic examination. After the formalin fixation, the specimens were stained by means of histopathologic techniques using the Levaditi and Hematoxylin Eosin stains. The ovary smears were also examined by the direct fluorescent antibody technique andlhe bacterial isolation was carried out in the Fletcher’s medium. The dark field direct microscopic examination was found tobe less sensitive in demonstrating the presence of leptospiresin the ovaries. In those specimens stained by the Lcvadititechnique, leptospires were visualized in different ovaryinternal structures, involving the interspace, pellucid zone andin the inner ovules. Through the histopathologic examination,typical morphologic alterations resembling acute infiamatoryprocess were found in 57% of ovaries examined.

  10. The multifunctional LigB adhesin binds homeostatic proteins with potential roles in cutaneous infection by pathogenic Leptospira interrogans.

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    Henry A Choy

    Full Text Available Leptospirosis is a potentially fatal zoonotic disease in humans and animals caused by pathogenic spirochetes, such as Leptospira interrogans. The mode of transmission is commonly limited to the exposure of mucous membrane or damaged skin to water contaminated by leptospires shed in the urine of carriers, such as rats. Infection occurs during seasonal flooding of impoverished tropical urban habitats with large rat populations, but also during recreational activity in open water, suggesting it is very efficient. LigA and LigB are surface localized proteins in pathogenic Leptospira strains with properties that could facilitate the infection of damaged skin. Their expression is rapidly induced by the increase in osmolarity encountered by leptospires upon transition from water to host. In addition, the immunoglobulin-like repeats of the Lig proteins bind proteins that mediate attachment to host tissue, such as fibronectin, fibrinogen, collagens, laminin, and elastin, some of which are important in cutaneous wound healing and repair. Hemostasis is critical in a fresh injury, where fibrinogen from damaged vasculature mediates coagulation. We show that fibrinogen binding by recombinant LigB inhibits fibrin formation, which could aid leptospiral entry into the circulation, dissemination, and further infection by impairing healing. LigB also binds fibroblast fibronectin and type III collagen, two proteins prevalent in wound repair, thus potentially enhancing leptospiral adhesion to skin openings. LigA or LigB expression by transformation of a nonpathogenic saprophyte, L. biflexa, enhances bacterial adhesion to fibrinogen. Our results suggest that by binding homeostatic proteins found in cutaneous wounds, LigB could facilitate leptospirosis transmission. Both fibronectin and fibrinogen binding have been mapped to an overlapping domain in LigB comprising repeats 9-11, with repeat 11 possibly enhancing binding by a conformational effect. Leptospirosis

  11. Proteína LIC10494 de Leptospira interrogans serovar Copenhageni: modelo estructural y regiones funcionales asociadas

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    Orlando Emilio Acevedo

    2012-04-01

    Full Text Available Protein LIC10494 of Leptospira interrogans serovar Copenhageni: structural model and associated functional regions. Objective.Predict by computational means the 3D structure of the antigenic protein LIC10494 and report associated important functional regionsfor its pathogenicity and immunogenicity. Materials and methods. We performed a computational analysis of the primary structure ofLIC10494 using the servers BLAST, PROTPARAM, PROTSCALE, DAS, SOSUI, TOPPRED, TMAP, TMpred, SPLIT4, PHDHTM,TMHMM2, HMMTOP2, GLOBPLOT and PROSITE. The secondary structure was obtained by consensus of the algorithms SOPM,PREDATOR GOR4, DPM and DSC. The approach to the tertiary structure was obtained using the algorithm MUSTER. The energyminimization was done using the AMBER94 force field of the Schrodinger suite of molecular analysis, and the stereochemistry andenergy model validation was performed by the RAMPAGE server. The final model was visualized using PyMol V.0,98. Results. Thisstudy proposes a computational model that describes the 3D structure of the hypothetical lipoprotein LIC10494 and agrees with previousexperimental reports; thus, our study demonstrates the existence of patterns that could play an important role in the pathogenicity andprotection of the bacteria against the host immune system; the presence of a disorganized region between amino acids 80 and 140, andof a transmembrane segment between amino acids 8 and 22. Conclusion. The coincidence between structural and functional segments suggests that our model can be used to predict certain aspects of the biological behaviour of the protein according to the pathogenic andimmunogenic characteristics of the bacteria.

  12. Structure-Function Analyses of the Interactions between Rab11 and Rab14 Small GTPases with Their Shared Effector Rab Coupling Protein (RCP)*

    Science.gov (United States)

    Lall, Patrick; Lindsay, Andrew J.; Hanscom, Sara; Kecman, Tea; Taglauer, Elizabeth S.; McVeigh, Una M.; Franklin, Edward; McCaffrey, Mary W.; Khan, Amir R.

    2015-01-01

    Rab GTPases recruit effector proteins, via their GTP-dependent switch regions, to distinct subcellular compartments. Rab11 and Rab25 are closely related small GTPases that bind to common effectors termed the Rab11 family of interacting proteins (FIPs). The FIPs are organized into two subclasses (class I and class II) based on sequence and domain organization, and both subclasses contain a highly conserved Rab-binding domain at their C termini. Yeast two-hybrid and biochemical studies have revealed that the more distantly related Rab14 also interacts with class I FIPs. Here, we perform detailed structural, thermodynamic, and cellular analyses of the interactions between Rab14 and one of the class I FIPs, the Rab-coupling protein (RCP), to clarify the molecular aspects of the interaction. We find that Rab14 indeed binds to RCP, albeit with reduced affinity relative to conventional Rab11-FIP and Rab25-FIP complexes. However, in vivo, Rab11 recruits RCP onto biological membranes. Furthermore, biophysical analyses reveal a noncanonical 1:2 stoichiometry between Rab14-RCP in dilute solutions, in contrast to Rab11/25 complexes. The structure of Rab14-RCP reveals that Rab14 interacts with the canonical Rab-binding domain and also provides insight into the unusual properties of the complex. Finally, we show that both the Rab coupling protein and Rab14 function in neuritogenesis. PMID:26032412

  13. Structure-Function Analyses of the Interactions between Rab11 and Rab14 Small GTPases with Their Shared Effector Rab Coupling Protein (RCP).

    Science.gov (United States)

    Lall, Patrick; Lindsay, Andrew J; Hanscom, Sara; Kecman, Tea; Taglauer, Elizabeth S; McVeigh, Una M; Franklin, Edward; McCaffrey, Mary W; Khan, Amir R

    2015-07-24

    Rab GTPases recruit effector proteins, via their GTP-dependent switch regions, to distinct subcellular compartments. Rab11 and Rab25 are closely related small GTPases that bind to common effectors termed the Rab11 family of interacting proteins (FIPs). The FIPs are organized into two subclasses (class I and class II) based on sequence and domain organization, and both subclasses contain a highly conserved Rab-binding domain at their C termini. Yeast two-hybrid and biochemical studies have revealed that the more distantly related Rab14 also interacts with class I FIPs. Here, we perform detailed structural, thermodynamic, and cellular analyses of the interactions between Rab14 and one of the class I FIPs, the Rab-coupling protein (RCP), to clarify the molecular aspects of the interaction. We find that Rab14 indeed binds to RCP, albeit with reduced affinity relative to conventional Rab11-FIP and Rab25-FIP complexes. However, in vivo, Rab11 recruits RCP onto biological membranes. Furthermore, biophysical analyses reveal a noncanonical 1:2 stoichiometry between Rab14-RCP in dilute solutions, in contrast to Rab11/25 complexes. The structure of Rab14-RCP reveals that Rab14 interacts with the canonical Rab-binding domain and also provides insight into the unusual properties of the complex. Finally, we show that both the Rab coupling protein and Rab14 function in neuritogenesis.

  14. The novel Rab11-FIP/Rip/RCP family of proteins displays extensive homo- and hetero-interacting abilities.

    Science.gov (United States)

    Wallace, Deborah M; Lindsay, Andrew J; Hendrick, Alan G; McCaffrey, Mary W

    2002-04-12

    The Rab11-FIP/Rip/RCP proteins are a recently described novel protein family, whose members interact with Rab GTPases that function in endosomal recycling. To date, five such proteins have been described in humans, all of which interact with Rab11, and one (RCP) also interacts with Rab4. Here, we characterise several of these proteins with respect to their ability to interact with Rab4, as well as their ability to self-interact, and to interact with each other. We now demonstrate that two of the family members-pp75/Rip11 and Rab11-FIP3 do not bind Rab4 and show that several members of the family can self-interact and interact with each other. These interactions primarily involve their C-terminal end which includes the Rab binding domain (RBD) that is contained within a predicted coiled-coil, or ERM motif. We identify a new (sixth) member of the protein family, which we propose to name Rab11-FIP4, and report the family evolutionary complexity and chromosomal distribution. Furthermore, we propose that the ability of these proteins to bind each other will be important in effecting membrane trafficking events by forming protein 'platforms,' regulated by Rab11 and/or Rab4 activity.

  15. 基于 RCP 的可扩展性应用平台研究与实现

    Institute of Scientific and Technical Information of China (English)

    黄德瑞; 王秋海

    2012-01-01

      RCP (Rich Client Platform)是基于 Eclipse 开发平台而推出的一套用来开发富客户端程序的应用框架。传统 B/S架构虽然在其程序部署,版本控制等方面有很大的优势,但是 C/S 也具备其独特的优势比如离线处理能力,低服务器负载等等。本文回顾了 Eclipse 以及 RCP 程序的特点,同时对比了基于 B/S 应用的特点。本研究结合 Eclipse 平台的特点提出一种应用于快速开发应用的可拓展性平台。

  16. Esterificacion quimioselectiva de fitosteroles de madera mediante lipasas

    National Research Council Canada - National Science Library

    Illanes, Andres; Alvarez, Lorena; Alvaro, Gregorio

    2008-01-01

    .... Ambas sustancias son muy similares, lo que impide su separacion por metodos fisicos, siendo la esterificacion selectiva de esta mezcla con esteres de acidos grasos mediante lipasas quimioselectivas...

  17. Production of reactive oxygen species and expression of inducible nitric oxide synthase in rat isolated Kupffer cells stimulated by Leptospira interrogans and Borrelia burgdorferi

    Institute of Scientific and Technical Information of China (English)

    Antonella Marangoni; Silvia Accardo; Rita Aldini; Massimo Guardigli; Francesca Cavrini; Vittorio Sambri; Marco Montagnani; Aldo Roda; Roberto Cevenini

    2006-01-01

    AIM: To evaluate the production of reactive oxygen species (ROS) and the expression of indudble nitric oxide synthase (iNOS) in rat isolated Kupffer cells (KCs) stimulated by Leptospira interrogans and Borrelia burgdorferi.METHODS: Rat Kupffer cells were separated by perfusion of the liver with 0.05% collagenase, and purified by Percoll gradients. Purified Kupffer cells were tested in vitro with alive L.interogans and B. burgdorferi preparations. The production of ROS was determined by chemiluminescence, whereas iNOS protein expression was evaluated by Western blot assay using anti-iNOS antibodies.RESULTS: B. burgdorferi and to a less extent L. interrogans induced ROS production with a peak 35 min after infection. The chemiluminescence signal progressively diminished and was undetectable by 180 min of incubation. Leptospirae and borreliae induced an increased iNOS expression in Kupffer cells that peaked at 6 hours and was still evident 22 h after infection.CONCLUSION: Both genera of spirochetes induced ROS and iNOS production in rat Kupffer cells. Since the cause of liver damage both in leptospiral as well as in borrelial infections are still unknown, we suggest that leptospira and borrelia damage of the liver can be initially mediated by oxygen radicals, and is then maintained at least in part by nitric oxide.

  18. Development of the leptospirosis by experimental infection in hamsters (Mesocricetus auratus with Leptospira interrogans serovar Canicola, strain LO4, by intact and scratched skin exposures

    Directory of Open Access Journals (Sweden)

    Carolina de Sousa Américo Batista

    2010-10-01

    Full Text Available The establishment and evolution of leptospirosis in hamster (Mesocricetus auratus by experimental infection with Leptospira interrogans serovar Canicola, LO4 strain, by intact and scratched skin exposures, having as control the intraperitoneal route, were evaluated. Hundred-twenty female hamsters distributed in two groups according to inoculation route (intact and scratched skin were used. Infectious inoculum was constituted by a pure culture of L. interrogans serovar Canicola (strain LO4, isolated from liver from a slaughtered swine in Londrina, Paraná state and typified by agglutinins adsortion technique with monoclonal antibody kit at the Royal Tropical Institute, Amsterdam, the Netherlands. The animals were observed twice a day during 21 days. Animals that died were necropsied and kidneys, liver, genital tract (uterus and ovaries and brain were aseptically collected. On the 21st post-inoculation day, surviving animals were euthanized. In these animals, serum samples were also collected by cardiac puncture to antileptospires agglutinins research using microscopic agglutination test (MAT. Fresh direct microscopy and microbiological culture were used for the detection of leptospires. Scratched skin route induced larger lethality when compared to intact skin route, with establishment and evolution of leptospirosis. On the other hand, intact skin route induced renal and/or genital carrier state more frequently. LO4 strain presented low immunogenic power, characterized by soroconversion at the MAT in only one inoculated animal.

  19. SEROPREVALENCE OF NINE LEPTOSPIRA INTERROGANS SEROVARS IN WILD CARNIVORES, UNGULATES, AND PRIMATES FROM A ZOO POPULATION IN A METROPOLITAN REGION OF CHILE.

    Science.gov (United States)

    Moreno-Beas, Eduardo; Abalos, Pedro; Hidalgo-Hermoso, Ezequiel

    2015-12-01

    Serum samples from 130 individuals representing 42 species of carnivores, ungulates, and primates from a population of captive mammals in Metropolitan Region in Chile were tested for antibodies against nine serovars of Leptospira interrogans using the microscopic agglutination test. Ten percent of the animals were seropositive to one or more serovars. Seroprevalence was significantly higher in ungulates (20.4%) compared to carnivores (3.8%) and primates (3.4%). There were no significant differences in seroprevalence among sex and age ranges. The most frequent serovar detected was Autumnalis, present in 53.4% of antibody-positive animals. Most positive animals had titers of ≤1 : 200, except for a maned wolf ( Chrysocyon brachyurus ) with titers of 1 : 400 against serovar Hardjo. To the authors' knowledge, this is the first report of Leptospira exposure detected in native endangered pudu ( Pudu puda ) and the first confirmation of exposure to L. interrogans in captive wild mammals in Chile. Leptospirosis should be considered as a differential diagnosis in future disease presentation for hepatitis or abortions in captive mammals in Chile.

  20. Research of Rapid Crack Propagation (RCP) Performance of Polyehylene Pressure Pipes%聚乙烯压力管材快速裂纹扩展(RCP)性能的研究

    Institute of Scientific and Technical Information of China (English)

    华晔; 武志军

    2014-01-01

    目前评价聚乙烯压力管材抗快速裂纹扩展(RCP)性能的测试方法有2种,全比例试验(FS)和小比例稳态试验(S4方法).相比FS方法,S4方法更简便和可控.研究了S4方法的影响因素,包括管材尺寸、样品安装时间、冲击速度等因素对RCP试验结果的影响.同时也研究了RCP性能和简支梁冲击强度及其他物理机械性能的关系.

  1. Projections of high resolution climate changes for South Korea using multiple-regional climate models based on four RCP scenarios. Part 1: surface air temperature

    Science.gov (United States)

    Suh, Myoung-Seok; Oh, Seok-Geun; Lee, Young-Suk; Ahn, Joong-Bae; Cha, Dong-Hyun; Lee, Dong-Kyou; Hong, Song-You; Min, Seung-Ki; Park, Seong-Chan; Kang, Hyun-Suk

    2016-05-01

    We projected surface air temperature changes over South Korea during the mid (2026-2050) and late (2076-2100) 21st century against the current climate (1981-2005) using the simulation results from five regional climate models (RCMs) driven by Hadley Centre Global Environmental Model, version 2, coupled with the Atmosphere- Ocean (HadGEM2-AO), and two ensemble methods (equal weighted averaging, weighted averaging based on Taylor's skill score) under four Representative Concentration Pathways (RCP) scenarios. In general, the five RCM ensembles captured the spatial and seasonal variations, and probability distribution of temperature over South Korea reasonably compared to observation. They particularly showed a good performance in simulating annual temperature range compared to HadGEM2-AO. In future simulation, the temperature over South Korea will increase significantly for all scenarios and seasons. Stronger warming trends are projected in the late 21st century than in the mid-21st century, in particular under RCP8.5. The five RCM ensembles projected that temperature changes for the mid/late 21st century relative to the current climate are +1.54°C/+1.92°C for RCP2.6, +1.68°C/+2.91°C for RCP4.5, +1.17°C/+3.11°C for RCP6.0, and +1.75°C/+4.73°C for RCP8.5. Compared to the temperature projection of HadGEM2-AO, the five RCM ensembles projected smaller increases in temperature for all RCP scenarios and seasons. The inter-RCM spread is proportional to the simulation period (i.e., larger in the late-21st than mid-21st century) and significantly greater (about four times) in winter than summer for all RCP scenarios. Therefore, the modeled predictions of temperature increases during the late 21st century, particularly for winter temperatures, should be used with caution.

  2. Future Climate Prediction of Urban Atmosphere in A Tropical Megacity: Utilization of RCP/SSP Scenarios with an Urban Growth Model

    Science.gov (United States)

    Darmanto, N. S.; Varquez, A. C. G.; Kanda, M.; Takakuwa, S.

    2016-12-01

    Economic development in Southeast Asia megacities leads to rapid transformation into more complicated urban configurations. These configurations, including building geometry, enhance aerodynamic drag thus reducing near-surface wind speeds. Roughness parameters representing building geometry, along with anthropogenic heat emissions, contribute to the formation of urban heat islands (UHI). All these have been reproduced successfully in the Weather Research and Forecasting (WRF) Model coupled with an improved single-layer urban canopy model incorporating a realistic distribution of urban parameters and anthropogenic heat emission in the Jakarta Greater Area. We apply this technology to climate change studies by introducing future urbanization defined by urban sprawl, vertical rise in buildings, and increase anthropogenic heat emission (AHE) due to population changes, into futuristic climate modelling. To simulate 2050s future climate, pseudo-global warming method was used which relied on current and ensembles of 5 CMIP5 GCMs for 2 representative concentration pathways (RCP), 2.6 and 8.5. To determine future urbanization level, 2050 population growth and energy consumption were estimated from shared socioeconomic pathways (SSP). This allows the estimation of future urban sprawl, building geometry, and AHE using the SLEUTH urban growth model and spatial growth assumptions. Two cases representing combinations of RCP and SSP were simulated in WRF: RCP2.6-SSP1 and RCP8.5-SSP3. Each case corresponds to best and worst-case scenarios of implementing adaptation and mitigation strategies, respectively. It was found that 2-m temperature of Jakarta will increase by 0.62°C (RCP2.6) and 1.44°C (RCP8.5) solely from background climate change; almost on the same magnitude as the background temperature increase of RCP2.6 (0.5°C) and RCP8.5 (1.2°C). Compared with previous studies, the result indicates that the effect of climate change on UHI in tropical cities may be lesser than

  3. Investigation of the Impact of a Heinrich-Event-like Abrupt Event Superimposed Onto the RCP 8.5 Scenario

    Science.gov (United States)

    Defrance, D.; Ramstein, G.; Dumas, C.; Charbit, S.

    2014-12-01

    The CMIP5/IPCC (AR5) projections of climate change showed the temperatures should increase by between 1.3 °C and 4.4 °C by 2100 and the sea level rise between 26 cm and 82 cm on average. Superimposed on these climatic and hydrologic trends associated with the RCP 8.5 most pessimistic scenario, it is important to investigate the possible effect of an abrupt event like an ice-sheet surge. This is first justified by the recent collapse of the Larsen B ice shelf which illustrates that a non-linear response of the cryosphere may occur in a warming world. Furthermore, in glacial periods, ice-sheets have been unstable and huge surges of icebergs did occur and deeply modified the climate. The aim of this presentation is to show the hypothetical climatic consequences of such a Heinrich-type event on future climate change. To achieve this goal, 3 scenarios of rapid ice-sheet deglaciation have been designed, corresponding to an additional sea level rise of around 3 m : - S1 corresponds to a contribution from Greenland only; - S2 from West-Antarctica only; - S3 from both ice-sheets. We use the global atmosphere-ocean general circulation model (OAGCM) IPSL-CM5A-LR. The freshwater perturbation is applied near the ice-sheet(s) during 20 years from 2050 to 2070 during an RCP8.5 perturbation. The RCP8.5 scenario is then continued until 2100 (without freshwater). For these types of scenarios, previous experiments for paleoclimatic cases help us diagnose the most vulnerable areas. The North Atlantic and the collapse of thermohaline circulation is one key issue, but far field teleconnections with the Asian monsoon will also be explored. We will investigate the timing and amplitude of the climate impacts due to the perturbations, which may be quite different in our 3 scenarios. These abrupt events consequences can be important for the populations and the consideration of rapid changes should improve the reliability of IPCC predictions.

  4. A new case of an inherited reciprocal translocation in cattle: rcp(13;26) (q24;q11).

    Science.gov (United States)

    Biltueva, Larisa; Kulemzina, Anastasia; Vorobieva, Nadezhda; Perelman, Polina; Kochneva, Marina; Zhidenova, Alexandra; Graphodatsky, Alexander

    2014-01-01

    This study reports on a unique balanced reciprocal chromosome translocation detected in a phenotypically normal cattle dam and her calf. CBG-, GTG-banding and FISH using bovine whole-chromosome and telomere probes were applied. The analyses showed that the breakpoints were located near to the centromere in chromosome 26 (q11) and exceptionally close to the telomere in chromosome 13 (q24). The whole euchromatin segment of chromosome 26 was translocated onto chromosome 13. The distal end of chromosome 13 was translocated to the subcentromeric region of chromosome 26. We describe this aberration as a balanced reciprocal translocation rcp(13;26) (q24;q11). It appears that this aberration was maternally derived and may have originated de novo in the dam. © 2014 S. Karger AG, Basel.

  5. Offshore wind energy climate projection using UPSCALE climate data under the RCP8.5 emission scenario

    CERN Document Server

    Gross, Markus

    2015-01-01

    Recently it was demonstrated how climate data can be utilized to estimate regional wind power densities. In particular it was shown that the quality of the global scale estimate compared well with regional high resolution studies and a link between surface temperature and moist density in the estimate was presented. In the present paper the methodology is tested further, to ensure that the results using one climate data set are reliable. This is achieved by extending the study to include four ensemble members. With the confidence that one instantiation is sufficient a climate change data set, which was also a result of the UPSCALE experiment, is analyzed. This, for the first time, provides a projection of future changes in wind power resources using this data set. This climate change data set is based on the Representative Concentration Pathways (RCP) 8.5 climate change scenario. This provides guidance for developers and policy makers to mitigate and adapt.

  6. Projection in Future Drought Hazard of South Korea Based on RCP Climate Change Scenario 8.5 Using SPEI

    Directory of Open Access Journals (Sweden)

    Byung Sik Kim

    2016-01-01

    Full Text Available The Standardized Precipitation Evapotranspiration Index (SPEI analysis was conducted using monthly precipitation data and temperature data on a 12.5 km × 12.5 km resolution based on a Representative Concentration Pathways (RCP 8.5 climate change scenario, and the characteristics of drought were identified by the threshold. In addition, the changes in drought severity and intensity were projected using the threshold based on the run-length concept and frequency analysis. As a result of the analysis, the probability density function of the total drought and maximum drought intensity moved the upper tail for the upcoming years, and the average drought intensity was also projected to become stronger in the future than in the present to the right side. Through this, it could be projected that the drought scale and frequency and the drought intensity will become severer over South Korea because of future climate change.

  7. Comparasion research of PFI and RCP financing mode%PFI和RCP融资模式的比较研究

    Institute of Scientific and Technical Information of China (English)

    杨涛; 徐霞; 于艳霞

    2011-01-01

    把当今比较流行的PFI融资模式和正处于理论研究阶段RCP模式在历史背景、特点、优缺点方面进行了详细的比较分析,以期给政府在非经营性或准经营性基础设施项目融资模式选择时提供一些参考意见。%According to analyzing and comparing the history background,merits and shortcomings etc.of PFI popular financing mode and RCP mode in the stage of theoretical research,the paper provides development direction and reference for government department choosi

  8. Projections of 2.0°C Warming over the Globe and China under RCP4.5

    Institute of Scientific and Technical Information of China (English)

    Zhang Ying

    2012-01-01

    The outputs of 17 models in the Coupled Model Intercomparison Project Phase 5 (CMIP5) are employed to investigate the temporal and spatial features of 2.0°C warming of the surface temperature over the globe and China under the Representative Concentration Pathways (RCP) 4.5 scenario. The simulations of the period 1860–1899 in the "historical" experiment are chosen as the baseline. The simulations for the 21st century in the RCP4.5 experiment are chosen as the future project. The multi-model ensemble mean (MME) shows that the global mean temperature would cross the 2.0°C warming threshold in 2047. Warming in most of the models would cross the threshold during 2030–2060. For local warming, high-latitude areas in the Northern Hemisphere show the fastest warming over the globe. Land areas warm substantially faster than the oceans. Most of the southern oceans would not exceed the 2.0°C warming threshold within the 21st century. Over China, surface warming is substantially faster than the global mean. The area-averaged warming would cross the 2.0°C threshold in 2034. Locally, Northwest China shows the fastest warming trend, followed by Central North China and Northeast China. Central China, East China, and South China are the last to cross the 2.0°C warming threshold. The diversity of the models is also estimated in this study. Generally, the spread among the models increases with time, and there is smaller spread among the models for the areas with the faster warming.

  9. RCP 技术在 Eclipse 与 NetBeans 上的分析比较

    Institute of Scientific and Technical Information of China (English)

    王锋

    2008-01-01

    RCP(Rich Client Platform)技术是通过定义微内核和相应的扩展机制,实现了基础表现层与业务层的近乎完全隔离,有效的改善了软件开发中的模块紧耦合,进一步提高了软件的可维护性、可复用性和可扩展性.但是目前富客户技术尚处于发展时期,在技术和实践方面还存在很多局限性,主要有微内核实现协议不够通用、插件机制各有不同、界面开发不能兼容等亟待解决的问题.本文以此为背景,研究分析了现有的几种富客户开发方法,并重点分析比较了其中的Eclipse RCP和NetBeans RCP,探索它们之间的异构性和相似性,深入研究了它们的体系结构和特点.分析表明,借助于新的技术和模式,目前对富客户开发技术的研究进展,为各领域软件构造提供了强有力的支持.

  10. Identification of Leptospira interrogans phospholipase C as a novel virulence factor responsible for intracellular free calcium ion elevation during macrophage death.

    Directory of Open Access Journals (Sweden)

    Jing-Fang Zhao

    Full Text Available BACKGROUND: Leptospira-induced macrophage death has been confirmed to play a crucial role in pathogenesis of leptospirosis, a worldwide zoonotic infectious disease. Intracellular free Ca(2+ concentration ([Ca(2+]i elevation induced by infection can cause cell death, but [Ca(2+]i changes and high [Ca(2+]i-induced death of macrophages due to infection of Leptospira have not been previously reported. METHODOLOGY/PRINCIPAL FINDINGS: We first used a Ca(2+-specific fluorescence probe to confirm that the infection of L. interrogans strain Lai triggered a significant increase of [Ca(2+]i in mouse J774A.1 or human THP-1 macrophages. Laser confocal microscopic examination showed that the [Ca(2+]i elevation was caused by both extracellular Ca(2+ influx through the purinergic receptor, P2X7, and Ca(2+ release from the endoplasmic reticulum, as seen by suppression of [Ca(2+]i elevation when receptor-gated calcium channels were blocked or P2X7 was depleted. The LB361 gene product of the spirochete exhibited phosphatidylinositol phospholipase C (L-PI-PLC activity to hydrolyze phosphatidylinositol-4,5-bisphosphate (PIP2 into inositol-1,4,5-trisphosphate (IP3, which in turn induces intracellular Ca(2+ release from endoplasmic reticulum, with the Km of 199 µM and Kcat of 8.566E-5 S(-1. Secretion of L-PI-PLC from the spirochete into supernatants of leptospire-macrophage co-cultures and cytosol of infected macrophages was also observed by Western Blot assay. Lower [Ca(2+]i elevation was induced by infection with a LB361-deficient leptospiral mutant, whereas transfection of the LB361 gene caused a mild increase in [Ca(2+]i. Moreover, PI-PLCs (PI-PLC-β3 and PI-PLC-γ1 of the two macrophages were activated by phosphorylation during infection. Flow cytometric detection demonstrated that high [Ca(2+]i increases induced apoptosis and necrosis of macrophages, while mild [Ca(2+]i elevation only caused apoptosis. CONCLUSIONS/SIGNIFICANCE: This study demonstrated that L

  11. FMN-coated fluorescent iron oxide nanoparticles for RCP-mediated targeting and labeling of metabolically active cancer and endothelial cells.

    Science.gov (United States)

    Jayapaul, Jabadurai; Hodenius, Michael; Arns, Susanne; Lederle, Wiltrud; Lammers, Twan; Comba, Peter; Kiessling, Fabian; Gaetjens, Jessica

    2011-09-01

    Riboflavin is an essential vitamin for cellular metabolism and is highly upregulated in metabolically active cells. Consequently, targeting the riboflavin carrier protein (RCP) may be a promising strategy for labeling cancer and activated endothelial cells. Therefore, Ultrasmall SuperParamagnetic Iron Oxide nanoparticles (USPIO) were adsorptively coated with the endogenous RCP ligand flavin mononucleotide (FMN), which renders them target-specific and fluorescent. The core diameter, surface morphology and surface coverage of the resulting FMN-coated USPIO (FLUSPIO) were evaluated using a variety of physico-chemical characterization techniques (TEM, DLS, MRI and fluorescence spectroscopy). The biocompatibility of FLUSPIO was confirmed using three different cell viability assays (Trypan blue staining, 7-AAD staining and TUNEL). In vitro evaluation of FLUSPIO using MRI and fluorescence microscopy demonstrated high labeling efficiency of cancer cells (PC-3, DU-145, LnCap) and activated endothelial cells (HUVEC). Competition experiments (using MRI and ICP-MS) with a 10- and 100-fold excess of free FMN confirmed RCP-specific uptake of the FLUSPIO by PC-3 cells and HUVEC. Hence, RCP-targeting via FMN may be an elegant way to render nanoparticles fluorescent and to increase the labeling efficacy of cancer and activated endothelial cells. This was shown for FLUSPIO, which due to their high T(2)-relaxivity, are favorably suited for MR cell tracking experiments and cancer detection in vivo. Copyright © 2011 Elsevier Ltd. All rights reserved.

  12. Changes in atmospheric blocking characteristics within Euro-Atlantic region and Northern Hemisphere as a whole in the 21st century from model simulations using RCP anthropogenic scenarios

    Science.gov (United States)

    Mokhov, Igor I.; Timazhev, Alexander V.; Lupo, Anthony R.

    2014-11-01

    An analysis of simulations using the IPSL-CM5 climate model of general circulation shows the ability of this model to reproduce the current climate of the main blocking characteristics obtained from reanalysis data, including number of blocking events and their duration, intensity and frequency. Possible changes of blocking characteristics in the Euro-Atlantic region (EA) and for the Northern Hemisphere (NH) as a whole are estimated from model simulations with the RCP2.6 and RCP8.5 scenarios for the 21st century. Results of the model simulations show a general increase in the blocking frequency for the EA in winter, summer and for the entire year during the 21st century for both analyzed RCP scenarios, while changes of the opposite sign are characteristic for NH as a whole. It is also noted that there is a tendency for an increase in the blocking intensity in the EA during the winter from model simulations with both analyzed RCP scenarios for the 21st century. A significant increase is obtained in the EA for the likelihood of extreme winter with the total blocking duration longer than seven weeks. Also, a similar tendency is characteristic for the EA summer.

  13. From Land Use to Land Cover: Restoring the Afforestation Signal in a Coupled Integrated Assessment - Earth System Model and the Implications for CMIP5 RCP Simulations

    Science.gov (United States)

    Di Vittorio, A. V.; Chini, L. P.; Bond-Lamberty, B. P.; Mao, J.; Shi, X.; Truesdale, J. E.; Craig, A.; Calvin, K. V.; Jones, A. D.; Collins, W.; Edmonds, J.; Hurtt, G. C.; Thornton, P. E.; Thomson, A. M.

    2014-12-01

    Climate projections depend on scenarios of fossil fuel emissions and land use change, and the IPCC AR5 parallel process assumes consistent climate scenarios across Integrated Assessment and Earth System Models (IAMs and ESMs). The CMIP5 project used a novel "land use harmonization" based on the Global Land use Model (GLM) to provide ESMs with consistent 1500-2100 land use trajectories generated by historical data and four IAM projections. A direct coupling of the Global Change Assessment Model (GCAM), GLM, and the Community ESM (CESM) has allowed us to characterize and partially address a major gap in the CMIP5 land coupling design: the lack of a corresponding land cover harmonization. The CMIP5 CESM global afforestation is only 22% of GCAM's 2005 to 2100 RCP4.5 afforestation. Likewise, only 17% of GCAM's 2040 RCP4.5 afforestation, and zero pasture loss, were transmitted to CESM within the directly coupled model. This is a problem because afforestation was relied upon to achieve RCP4.5 climate stabilization. GLM modifications within the directly coupled model did not increase CESM afforestation. Modifying the CESM land use translator in addition to GLM, however, enabled CESM to simulate 66% of GCAM's afforestation in 2040, and 94% of GCAM's pasture loss as grassland and shrubland losses. This additional afforestation increases vegetation carbon gain by 19 PgC and decreases atmospheric CO2 gain by 8 ppmv from 2005 to 2040, implying different RCP4.5 climate scenarios between CMIP5 GCAM and CESM. Although the IAMs and ESMs were not expected to have exactly the same climate forcing, due in part to different terrestrial carbon cycles and atmospheric radiation algorithms, the ESMs were expected to project climates representative of the RCP scenarios. Similar land cover inconsistencies exist in other CMIP5 model results, primarily because land cover information is not shared between IAM and ESM models. High RCP4.5 afforestation might also contribute to inconsistencies as

  14. From land use to land cover: restoring the afforestation signal in a coupled integrated assessment – earth system model and the implications for CMIP5 RCP simulations

    Directory of Open Access Journals (Sweden)

    A. V. Di Vittorio

    2014-05-01

    Full Text Available Climate projections depend on scenarios of fossil fuel emissions and land use change, and the IPCC AR5 parallel process assumes consistent climate scenarios across Integrated Assessment and Earth System Models (IAMs and ESMs. The CMIP5 project used a novel "land use harmonization" based on the Global Land use Model (GLM to provide ESMs with consistent 1500–2100 land use trajectories generated by historical data and four IAM projections. A direct coupling of the Global Change Assessment Model (GCAM, GLM, and the Community ESM (CESM has allowed us to characterize and partially address a major gap in the CMIP5 land coupling design: the lack of a corresponding land cover harmonization. The CMIP5 CESM global afforestation is only 22% of GCAM's 2005 to 2100 RCP4.5 afforestation. Likewise, only 17% of GCAM's 2040 RCP4.5 afforestation, and zero pasture loss, were transmitted to CESM within the directly coupled model. This is a problem because afforestation was relied upon to achieve RCP4.5 climate stabilization. GLM modifications within the directly coupled model did not increase CESM afforestation. Modifying the land use translator in addition to GLM, however, enabled CESM to simulate 66% of GCAM's afforestation in 2040, and 94% of GCAM's pasture loss as grassland and shrubland losses. This additional afforestation increases vegetation carbon gain by 19 PgC and decreases atmospheric CO2 gain by 8 ppmv from 2005 to 2040, implying different RCP4.5 climate scenarios between CMIP5 GCAM and CESM. Although the IAMs and ESMs were not expected to have exactly the same climate forcing, due in part to different terrestrial carbon cycles and atmospheric radiation algorithms, the ESMs were expected to project climates representative of the RCP scenarios. Similar land cover inconsistencies exist in other CMIP5 model results, primarily because land cover information is not shared between models. High RCP4.5 afforestation might also contribute to inconsistencies as

  15. Determination of the genus-specific antigens in outer membrane proteins from the strains of Leptospira interrogans and Leptospira biflexa with different virulence

    Institute of Scientific and Technical Information of China (English)

    罗依惠; 严杰; 毛亚飞; 李淑萍

    2004-01-01

    Objective:To determine the existence of genus-specific antigens in outer membrane proteins (OMPs) of leptospira with different virulence. Methods: Microscope agglutination test (MAT) was applied to detect the agglutination between commercial rabbit antiserum against leptospiral genus-specific TR/Patoc I antigen and 17 strains of Leptospira interrongans belonging to 15 serogroups and 2 strains of Leptospira biflexa belonging to 2 serogroups.The outer envelopes (OEs) of L.interrogans serogroup Icterohaemorrhagiae serovar lai strain lai (56601) with strong virulence and serogroup Pomona serovar pomona strain Luo (56608) with low virulence,and L.biflexa serogroup Semaranga serovar patoc strain Patoc I without virulence were prepared by using the method reported in Auran et al.(1972).OMPs in the OEs were obtained by treatment with sodium deoxycholate. SDS-PAGE and western blot were used for analyzing the features of the OMPs on electrophoretic pattern and the immunoreactivity to the antiserum against TR/Patoc I antigen, respectively. Results:All the tested strains belonging to different leptospiral serogroups agglutinated to the antiserum against leptospiral genus-specific TR/Patoc I antigen with agglutination titers ranging from 1:256-1:512. A similar SDS-PAGE pattern of the OMPs from the three strains of leptospira with different virulence was shown and the molecular weight of a major protein fragment in the OMPs was found to be approximately 60 KDa.A positive protein fragment with approximately 32 KDa confirmed by Western blot,was able to react with the antiserum against leptospiral genus-specific TR/Patoc I antigen, and was found in each the OMPs of the three stains of leptospira.Conclusion: There are genus-specific antigens on the surface of L.interrogans and L.biflexa. The OMP with molecular weight of 32 KDa may be one of the genus-specific protein antigens of leptospira.

  16. A model system for studying the transcriptomic and physiological changes associated with mammalian host-adaptation by Leptospira interrogans serovar Copenhageni.

    Directory of Open Access Journals (Sweden)

    Melissa J Caimano

    2014-03-01

    Full Text Available Leptospirosis, an emerging zoonotic disease with worldwide distribution, is caused by spirochetes belonging to the genus Leptospira. More than 500,000 cases of severe leptospirosis are reported annually, with >10% of these being fatal. Leptospires can survive for weeks in suitably moist conditions before encountering a new host. Reservoir hosts, typically rodents, exhibit little to no signs of disease but shed large numbers of organisms in their urine. Transmission occurs when mucosal surfaces or abraded skin come into contact with infected urine or urine-contaminated water or soil. In humans, leptospires can cause a variety of clinical manifestations, ranging from asymptomatic or mild fever to severe icteric (Weil's disease and pulmonary haemorrhage. Currently, little is known about how Leptospira persist within a reservoir host. Prior in vitro studies have suggested that leptospires alter their transcriptomic and proteomic profiles in response to environmental signals encountered during mammalian infection. However, no study has examined gene expression by leptospires within a mammalian host-adapted state. To obtain a more faithful representation of how leptospires respond to host-derived signals, we used RNA-Seq to compare the transcriptome of L. interrogans cultivated within dialysis membrane chambers (DMCs implanted into the peritoneal cavities of rats with that of organisms grown in vitro. In addition to determining the relative expression levels of "core" housekeeping genes under both growth conditions, we identified 166 genes that are differentially-expressed by L. interrogans in vivo. Our analyses highlight physiological aspects of host adaptation by leptospires relating to heme uptake and utilization. We also identified 11 novel non-coding transcripts that are candidate small regulatory RNAs. The DMC model provides a facile system for studying the transcriptional and antigenic changes associated with mammalian host

  17. Determination of the genus-specific antigens in outer membrane proteins from the strains of Leptospira interrogans and Leptospira biflexa with different virulence

    Institute of Scientific and Technical Information of China (English)

    罗依惠; 严杰; 毛亚飞; 李淑萍

    2004-01-01

    Objective: To determine the existence of genus-specific antigens in outer membrane proteins (OMPs) of leptospira with different virulence. Methods: Microscope agglutination test (MAT) was applied to detect the agglutination between commercial rabbit antiserum against leptospiral genus-specific TR/Patoc I antigen and 17 strains of Leptospira interrongans belonging to 15 serogroups and 2 strains of Leptospira biflexa belonging to 2 serogroups. The outer envelopes (OEs) of L.interrogans serogroup Icterohaemorrhagiae serovar lai strain lai (56601) with strong virulence and serogroup Pomona serovar pomona strain Luo (56608) with low virulence, and L.biflexa serogroup Semaranga serovar patoc strain Patoc I without virulence were prepared by using the method reported in Auran et al.(1972). OMPs in the OEs were obtained by treatment with sodium deoxycholate. SDS-PAGE and western blot were used for analyzing the features of the OMPs on electrophoretic pattern and the immunoreactivity to the antiserum against TR/Patoc I antigen, respectively. Results: All the tested strains belonging to different leptospiral serogroups agglutinated to the antiserum against leptospiral genus-specific TR/Patoc I antigen with agglutination titers ranging from 1:256-1:512. A similar SDS-PAGE pattern of the OMPs from the three strains of leptospira with different virulence was shown and the molecular weight of a major protein fragment in the OMPs was found to be approximately 60 KDa. A positive protein fragment with approximately 32 KDa confirmed by Western blot, was able to react with the antiserum against leptospiral genus-specific TR/Patoc I antigen, and was found in each the OMPs of the three stains of leptospira. Conclusion: There are genus-specific antigens on the surface of L.interrogans and L.biflexa. The OMP with molecular weight of 32 KDa may be one of the genus-specific protein antigens of leptospira.

  18. Seropositivity to Leptospira interrogans serovar Bratislava associated to reproductive problems without significant biochemical or hematological alterations in horses Soropositividade para Leptospira interrogans serovar Bratislava associada a falhas reprodutivas sem alterações hematológicas e bioquímicas significativas em cavalos

    Directory of Open Access Journals (Sweden)

    Melissa Pinna

    2010-10-01

    Full Text Available The objective was to study haematological and biochemical alterations associated to seropositivity to Leptospira interrogans serovar Bratislava infection in horses with reproductive alterations, such as neonatal deaths, embryonic deaths and abortions. A flock of mares with poor reproductive performance was studied. Eighty-two (58.6% were seropositive (titre 200; 72 of those (87.8% for Bratislava. Slight haematological and biochemical alterations were observed, being more frequent (PO objetivo deste trabalho foi estudar alterações hematológicas e bioquímicas associadas à soropositividade para Leptospira interrogans sorovar Bratislava em cavalos com alterações reprodutivas, tais como mortes neonatais, absorção embrionária e abortamentos. Um rebanho de éguas com baixos índices reprodutivos foi estudado. Oitenta e duas (58,6% foram soropositivas (títulos 200, sendo 72 destas (87,8% para Bratislava. Foram observadas poucas alterações hematológicas e bioquímicas, mais frequentes (P<0,05 em éguas soropositivas do que soronegativas. Cavalos soropositivos para Bratislava não tinham alterações graves nos valores hematológicos e bioquímicos. Esses achados reforçam que esse sorovar seja adaptado de cavalos e cause apenas sintomas brandos, associados a falhas reprodutivas.

  19. Effect of exposure to Neospora caninum, Salmonella, and Leptospira interrogans serovar Hardjo on the economic performance of Irish dairy herds.

    Science.gov (United States)

    O' Doherty, E; Sayers, R; O' Grady, L; Shalloo, L

    2015-04-01

    The objective of the current study was to quantify the effects of exposure to Salmonella, Neospora caninum, and Leptospira interrogans serovar Hardjo (L. hardjo) on dairy farm profitability and to simulate the effect of vaccination for Salmonella and L. hardjo on dairy farm profitability. The production effects associated with exposure to each of these pathogens in study herds were defined under 3 categories: (1) milk production effects, (2) reproduction effects (including culling), and (3) mortality effects. The production effects associated with exposure to Salmonella, N. caninum, and L. hardjo were incorporated into the Moorepark Dairy Systems Model. In the analysis, herds negative for exposure to Salmonella, N. caninum, and L. hardjo were assumed baseline herds, with all results presented relative to this base. In simulations examining the effect of vaccination for Salmonella and L. hardjo on farm profitability, vaccinated herds (vaccination costs included) were considered as baseline herds and results were presented relative to this base. Total annual profits in unvaccinated herds were reduced by €77.31, €94.71, and €112.11 per cow at milk prices of €0.24, €0.29, and €0.34/L, respectively, as a result of exposure to Salmonella. In the current study, herds positive for exposure to Salmonella recorded a 316-kg reduction in milk yield, whereas no association was detected between exposure to N. caninum or L. hardjo and milk production. Exposure to both N. caninum and L. hardjo was associated with compromised reproductive performance. Herds positive for exposure to N. caninum and Salmonella had greater rates of adult cow mortality and calf mortality, respectively. Vaccination for both Salmonella and L. hardjo was associated with improved performance in study herds. Exposure to N. caninum resulted in a reduction in annual farm profits of €11.55, €12, and €12.44 per cow at each milk price, whereas exposure to L. hardjo resulted in a reduction in

  20. Projecting potential distribution of Eucryptorrhynchus scrobiculatus Motschulsky and E. brandti (Harold) under historical climate and RCP 8.5 scenario.

    Science.gov (United States)

    Ji, Yingchao; Luo, Wen; Zhang, Ganyu; Wen, Junbao

    2017-08-22

    Ailanthus altissima (Mill.) Swingle and its variant A. altissima var. Qiantouchun are notorious invasive weeds. Two weevils, Eucryptorrhynchus scrobiculatus (ESC) and E. brandti (EBR) are considered as candidates for biological control of A. altissima. The aim of this study was to model the potential distributions of ESC and EBR using CLIMEX 4.0. The projected potential distributions of ESC and EBR included almost all current distribution areas of A. altissima, except Southeast Asia. Under historical climate, potential distribution area of EBR is larger than that of ESC, 46.67 × 10(6) km(2) and 35.65 × 10(6) km(2), respectively. For both ESC and EBR, climate change expanded the northern boundary of potential distributions northward approximately 600 km by the middle of 21st century, and 1000 km by the end of 21st century under RCP 8.5. However, the suitable range decreased to the south in the Southern Hemisphere because of heat stress. The modelled potential distributions of ESC and EBR in the United States demonstrated that the climate was suitable for both weevils. Therefore, considering only climate suitability, both ESC and EBR can be considered as potential biological control agents against A. altissima with some confidence that climatic conditions are likely suitable.

  1. A new case of reciprocal translocation in a young bull: rcp(11;21)(q28;q12).

    Science.gov (United States)

    Molteni, L; Perucatti, A; Iannuzzi, A; Di Meo, G P; De Lorenzi, L; De Giovanni, A; Incarnato, D; Succi, G; Cribiu, E; Eggen, A; Iannuzzi, L

    2007-01-01

    Routine cytogenetic investigations of the Chianina cattle (BTA) breed revealed the presence of longer and smaller chromosomes than the largest (BTA1) and smallest (BTA29) chromosomes in the cells of a young, normal-looking bull used for reproduction. Application of both RBA-banding and Ag-NOR techniques, as well as the use of the FISH technique and specific molecular markers of both BTA11 (IL1B, ASS and LGB) and BTA21 (SERPINA and D21S45) established that these two abnormal chromosomes were the product of a reciprocal translocation between BTA11 and BTA21. Both der(11) and der(21) were C-band positive and the chromosome regions affected were rcp(11;21)(q28;q12). The young bull had a normal body conformation, including external genitalia, normal levels of testosterone (as in the control) and non-detectable levels of both 17 beta-estradiol and progesterone (as in the control). The animal never showed libido in the presence of both males and females in oestrus. After slaughter at 18 months, histological evaluation revealed normal organized testes, seminiferous tubules and epididymis but with poor proliferative germ cells consisting mainly of spermatogonia, middle pachytene spermatocytes and early spermatids with late spermatids and spermatozoa being very rare. Copyright 2007 S. Karger AG, Basel.

  2. Future PMP Estimation in Korea under AR5 RCP 8.5 climate change scenarios and its Changes Cause Analysis

    Science.gov (United States)

    Kim, S.; Lee, J.; Okjeong, L.; Bogyeong, C.; Park, M. W.

    2015-12-01

    In this presentation, Korea's probable maximum precipitations (PMPs) which reflects all of the storm data until recently are calculated, and are compared to the existing PMPs which were calculated at 2000. In Korea, abnormal weather phenomena such as typhoon Rusa and Maemi, and the extreme rainfall event occurred on the east coast of the northern region, that can have a significant impact on the PMP estimation, have frequently happened since 2000. After selecting 240 major storm events from 1973 to 2012, new PMPs are proposed with respect to storm areas (25, 100, 225, 400, 900, 2025, 4900, 10000 and 19600 km2) and storm durations (1, 2, 4, 6, 8, 12, 18, 24, 48 and 72 hours) using the Korea hydro-meteorological method. After estimating future PMPs using future rainfall and dew point temperature information under the Korea Meteorological Administration AR5 RCP 8.5, changes in the PMPs under climate change will be investigated by comparison with present and future PMPs. By separating the changes in PMPs under climate change into the changes caused by rainfall and dew point temperature, the relative impact of future rainfall and dew point temperature information under climate change on future PMPs is quantified. This research was supported by a grant 'Development of the Evaluation Technology for Complex Causes of Inundation Vulnerability and the Response Plans in Coastal Urban Areas for Adaptation to Climate Change' [MPSS-NH-2015-77] from the Natural Hazard Mitigation Research Group, Ministry of Public Safety and Security of Korea.

  3. Coupled wave-atmosphere-ocean modeling under RCP8.5 emissions during the 21st century

    Science.gov (United States)

    de Vries, Hylke; Semedo, Alvaro; Sterl, Andreas

    2016-04-01

    Most climate models have no explicit treatment of surface ocean waves. As a result, wave climatologies are usually obtained off-line, using atmospheric forcing as input. In recent years attempts have been made to incorporate surface ocean wave models into GCMs, giving the possibility of interactions between components. In the presence of waves the atmospheric winds are modified due to different drag-coefficient and breaking waves will have also influence vertical ocean mixing. As such the waves may play a role in solving long-standing ocean and atmospheric biases. Recently we have coupled the global wave model WAM into EC-Earth GCM (v3.0.1). EC-Earth is based on the IFS atmospheric model and the NEMO/LIM ocean/sea ice model. First results are presented from a 140-year (1961-2100) continuous transient, coupled simulation under RCP8.5 emission scenario. As the modelled climate warms during the transient period, (arctic) sea-ice gradually diminishes, allowing surface waves to penetrate to higher and higher latitudes. Eventually Arctic sea-ice completely disappears in the simulation in late summer. We will discuss swell and wind-wave climatologies, Stokes drift patterns as well as other relevant wave parameters.

  4. Three dimensional calculations of the primary coolant flow in a 900 MW PWR vessel. Numerical simulation of the accurate RCP start-up flow rate

    Energy Technology Data Exchange (ETDEWEB)

    Martin, A.; Alvarez, D.; Cases, F.; Stelletta, S. [Electricite de France (EDF), 78 - Chatou (France). Lab. National d`Hydraulique

    1997-06-01

    This report explains the last results about the mixing in the 900 MW PWR vessels. The accurate fluid flow transient, induced by the RCP starting-up, is represented. In a first time, we present the Thermalhydraulic Finite Element Code N3S used for the 3D numerical computations. After that, results obtained for one reactor operation case are given. This case is dealing with the transient mixing of a clear plug in the vessel when one primary pump starts-up. A comparison made between two injection modes; a steady state fluid flow conditions or the accurate RCP transient fluid flow conditions. The results giving the local minimum of concentration and the time response of the mean concentration at the core inlet are compared. The results show the real importance of the unsteadiness characteristics of the fluid flow transport of the clear water plug. (author) 12 refs.

  5. Prediction of abundance of arthropods according to climate change scenario RCP 4.5 and 8.5 in South Korea

    Directory of Open Access Journals (Sweden)

    Cheol Min Lee

    2016-06-01

    Full Text Available Abundance and diversity of arthropods were projected according to climate warming in South Korea. The taxa highly linked with temperature were selected for the projection. The values of abundance and richness were estimated using the mean values of abundance and richness in each temperature range. Temperature changes were based on the RCP (Representative Concentration Pathway 4.5 and RCP 8.5, and the abundance and richness during two periods (2011 -2015, 2056 -2065 were projected. From these projected results, change of other common taxa (> 1% occurrence were qualitatively predicted (i.e., decrease or increase. The projections showed that 45 of a total of 73 taxa will increase, 6 will change a little and 24 will decrease: the number of taxa that were expected to increase was two times more than the number of taxa that were expected to decrease. However, the overall abundance and diversity of arthropods were expected to decline as the temperature rises.

  6. PREPARATION OF POLYMER—SUPPORTED RCpCpTiCl2 AND APPLICATION IN CATALYTIC ISOMERIZATION OF 1,5—HEXADIENE

    Institute of Scientific and Technical Information of China (English)

    QIANYanlong; HONGKunlun; 等

    1993-01-01

    Polymer-supported RCpCpTiCl2(Cp=η5-C5H4;RCp=η5-RC5H4)have been prepared and reduced by i-C3H7MgBr in situ,then used in catalytic isomerization of 1,5-hexadiene.The isomerization of 1,5-hexadiene leads to a mixture of cyclic and linear products.The ratio of cyclic to linear product depends on the nature of the supporter and the substituent on the cyclopentadienyl ring.The former contributes the most,for example,silica supported Cp2 TiCl2 gave cyclic-linear ratio of 99/1,while for polymer-supported Cp2TiCl2 species,linear compounds are the major products and the ratio of cyclic to linear product changes with different R groups on the cyclopentadienyl ring.

  7. Measuring Progress on the Control of Porcine Reproductive and Respiratory Syndrome (PRRS) at a Regional Level: The Minnesota N212 Regional Control Project (Rcp) as a Working Example.

    Science.gov (United States)

    Valdes-Donoso, Pablo; Jarvis, Lovell S; Wright, Dave; Alvarez, Julio; Perez, Andres M

    2016-01-01

    Due to the highly transmissible nature of porcine reproductive and respiratory syndrome (PRRS), implementation of regional programs to control the disease may be critical. Because PRRS is not reported in the US, numerous voluntary regional control projects (RCPs) have been established. However, the effect of RCPs on PRRS control has not been assessed yet. This study aims to quantify the extent to which RCPs contribute to PRRS control by proposing a methodological framework to evaluate the progress of RCPs. Information collected between July 2012 and June 2015 from the Minnesota Voluntary Regional PRRS Elimination Project (RCP-N212) was used. Demography of premises (e.g. composition of farms with sows = SS and without sows = NSS) was assessed by a repeated analysis of variance. By using general linear mixed-effects models, active participation of farms enrolled in the RCP-N212, defined as the decision to share (or not to share) PRRS status, was evaluated and used as a predictor, along with other variables, to assess the PRRS trend over time. Additionally, spatial and temporal patterns of farmers' participation and the disease dynamics were investigated. The number of farms enrolled in RCP-N212 and its geographical coverage increased, but the proportion of SS and NSS did not vary significantly over time. A significant increasing (pRCP-N212, active participation is not ensured. By evaluating the effect of participation on the occurrence of PRRS, the value of sharing information among producers may be demonstrated, in turn justifying the existence of RCPs.

  8. Projections of high resolution climate changes for South Korea using multiple-regional climate models based on four RCP scenarios. Part 2: precipitation

    Science.gov (United States)

    Oh, Seok-Geun; Suh, Myoung-Seok; Lee, Young-Suk; Ahn, Joong-Bae; Cha, Dong-Hyun; Lee, Dong-Kyou; Hong, Song-You; Min, Seung-Ki; Park, Seong-Chan; Kang, Hyun-Suk

    2016-05-01

    Precipitation changes over South Korea were projected using five regional climate models (RCMs) with a horizontal resolution of 12.5 km for the mid and late 21st century (2026-2050, 2076- 2100) under four Representative Concentration Pathways (RCP) scenarios against present precipitation (1981-2005). The simulation data of the Hadley Centre Global Environmental Model version 2 coupled with the Atmosphere-Ocean (HadGEM2-AO) was used as boundary data of RCMs. In general, the RCMs well simulated the spatial and seasonal variations of present precipitation compared with observation and HadGEM2-AO. Equal Weighted Averaging without Bias Correction (EWA_NBC) significantly reduced the model biases to some extent, but systematic biases in results still remained. However, the Weighted Averaging based on Taylor's skill score (WEA_Tay) showed a good statistical correction in terms of the spatial and seasonal variations, the magnitude of precipitation amount, and the probability density. In the mid-21st century, the spatial and interannual variabilities of precipitation over South Korea are projected to increase regardless of the RCP scenarios and seasons. However, the changes in area-averaged seasonal precipitation are not significant due to mixed changing patterns depending on locations. Whereas, in the late 21st century, the precipitation is projected to increase proportionally to the changes of net radiative forcing. Under RCP8.5, WEA_Tay projects the precipitation to be increased by about +19.1, +20.5, +33.3% for annual, summer and winter precipitation at 1-5% significance levels, respectively. In addition, the probability of strong precipitation (≥ 15 mm d-1) is also projected to increase significantly, particularly in WEA_Tay under RCP8.5.

  9. RCP-driven α5β1 recycling suppresses Rac and promotes RhoA activity via the RacGAP1–IQGAP1 complex

    Science.gov (United States)

    Jacquemet, Guillaume; Green, David M.; Bridgewater, Rebecca E.; von Kriegsheim, Alexander; Humphries, Martin J.; Norman, Jim C.

    2013-01-01

    Inhibition of αvβ3 or expression of mutant p53 promotes invasion into fibronectin (FN)-containing extracellular matrix (ECM) by enhancing Rab-coupling protein (RCP)–dependent recycling of α5β1 integrin. RCP and α5β1 cooperatively recruit receptor tyrosine kinases, including EGFR1, to regulate their trafficking and downstream signaling via protein kinase B (PKB)/Akt, which, in turn, promotes invasive migration. In this paper, we identify a novel PKB/Akt substrate, RacGAP1, which is phosphorylated as a consequence of RCP-dependent α5β1 trafficking. Phosphorylation of RacGAP1 promotes its recruitment to IQGAP1 at the tips of invasive pseudopods, and RacGAP1 then locally suppresses the activity of the cytoskeletal regulator Rac and promotes the activity of RhoA in this subcellular region. This Rac to RhoA switch promotes the extension of pseudopodial processes and invasive migration into FN-containing matrices, in a RhoA-dependent manner. Thus, the localized endocytic trafficking of α5β1 within the tips of invasive pseudopods elicits signals that promote the reorganization of the actin cytoskeleton, protrusion, and invasion into FN-rich ECM. PMID:24019536

  10. RCP-driven α5β1 recycling suppresses Rac and promotes RhoA activity via the RacGAP1-IQGAP1 complex.

    Science.gov (United States)

    Jacquemet, Guillaume; Green, David M; Bridgewater, Rebecca E; von Kriegsheim, Alexander; Humphries, Martin J; Norman, Jim C; Caswell, Patrick T

    2013-09-16

    Inhibition of αvβ3 or expression of mutant p53 promotes invasion into fibronectin (FN)-containing extracellular matrix (ECM) by enhancing Rab-coupling protein (RCP)-dependent recycling of α5β1 integrin. RCP and α5β1 cooperatively recruit receptor tyrosine kinases, including EGFR1, to regulate their trafficking and downstream signaling via protein kinase B (PKB)/Akt, which, in turn, promotes invasive migration. In this paper, we identify a novel PKB/Akt substrate, RacGAP1, which is phosphorylated as a consequence of RCP-dependent α5β1 trafficking. Phosphorylation of RacGAP1 promotes its recruitment to IQGAP1 at the tips of invasive pseudopods, and RacGAP1 then locally suppresses the activity of the cytoskeletal regulator Rac and promotes the activity of RhoA in this subcellular region. This Rac to RhoA switch promotes the extension of pseudopodial processes and invasive migration into FN-containing matrices, in a RhoA-dependent manner. Thus, the localized endocytic trafficking of α5β1 within the tips of invasive pseudopods elicits signals that promote the reorganization of the actin cytoskeleton, protrusion, and invasion into FN-rich ECM.

  11. Installation and Commissioning of RCP Cartridge for Nuclear Power Stations%核电厂主泵密封系统的安装与调试

    Institute of Scientific and Technical Information of China (English)

    霍亚邦; 王玉旭

    2011-01-01

    The installation process and method for the cooling pump sealing system of CPR1000 nuclear power plant reactor is studied, and various cases occurred during the installation and commisioning of RCP sealing systems are analyzed. The scheme for flushing of the nuclear loop in the various cases is developed to provide a reference for the installation and commissioning of RCP sealing system in the coming CPR1000 project.%研究CPR1000堆型核电厂反应堆冷却剂泵(RCP)密封安装工艺流程及方法,对RCP密封系统安装和调试过程中的各种情况进行分析,制定各种情况下核回路冲洗采取的特殊处理方案,为后续CPR1000项目RCP密封系统安装调试提供参考.

  12. Desarrollo comunicación Alfa Arduino mediante Scada

    OpenAIRE

    Trallero Calvo, Jorge

    2015-01-01

    El siguiente proyecto pretende crear un sistema IoT (Internet of Things) girando en torno a Arduino, demostrando de este modo la facilidad de creación de proyectos y fiabilidad que nos aporta Arduino. Mediante una comunicación TCP/IP se procede a crear un sistema el cual es capaz de registrar valores a una cierta distancia mediante protocolos de procesos y comunicación (Telemedida) y de este modo monitorizar la temperatura en un punto determinado, sin estar limitado por la t...

  13. Desarrollo de un sistema de videoconferencia mediante WebRTC

    OpenAIRE

    Ibarrola Lerga, Josu

    2016-01-01

    El objetivo de este proyecto fin de carrera consiste en el desarrollo de un sistema de videoconferencia mediante WebRTC. Para realizar dicho desarrollo crearemos una página mediante el lenguaje de programación HTML5 y la tecnología que nos brinda WebRTC, todo ello dentro del marco de trabajo de Bootstrap, consiguiendo así una página web apta para cualquier dispositivo de visualización que se utilice. Debido a que se trata de una tecnología nueva que el autor desconoce, para pod...

  14. Fine Mapping of the Interaction between C4b-Binding Protein and Outer Membrane Proteins LigA and LigB of Pathogenic Leptospira interrogans.

    Science.gov (United States)

    Breda, Leandro C D; Hsieh, Ching-Lin; Castiblanco Valencia, Mónica M; da Silva, Ludmila B; Barbosa, Angela S; Blom, Anna M; Chang, Yung-Fu; Yung-Fu, Chang; Isaac, Lourdes

    2015-01-01

    The complement system consists of more than 40 proteins that participate in the inflammatory response and in pathogen killing. Complement inhibitors are necessary to avoid the excessive consumption and activation of this system on host cells. Leptospirosis is a worldwide zoonosis caused by spirochetes from the genus Leptospira. Pathogenic leptospires are able to escape from complement activation by binding to host complement inhibitors Factor H [FH] and C4b-binding protein (C4BP) while non-pathogenic leptospires are rapidly killed in the presence of fresh serum. In this study, we demonstrate that complement control protein domains (CCP) 7 and 8 of C4BP α-chain interact with the outer membrane proteins LcpA, LigA and LigB from the pathogenic leptospire L. interrogans. The interaction between C4BP and LcpA, LigA and LigB is sensitive to ionic strength and inhibited by heparin. We fine mapped the LigA and LigB domains involved in its binding to C4BP and heparin and found that both interactions are mediated through the bacterial immunoglobulin-like (Big) domains 7 and 8 (LigA7-8 and LigB7-8) of both LigA and LigB and also through LigB9-10. Therefore, C4BP and heparin may share the same binding sites on Lig proteins.

  15. Offshore Wind Energy Climate Projection Using UPSCALE Climate Data under the RCP8.5 Emission Scenario.

    Science.gov (United States)

    Gross, Markus; Magar, Vanesa

    2016-01-01

    In previous work, the authors demonstrated how data from climate simulations can be utilized to estimate regional wind power densities. In particular, it was shown that the quality of wind power densities, estimated from the UPSCALE global dataset in offshore regions of Mexico, compared well with regional high resolution studies. Additionally, a link between surface temperature and moist air density in the estimates was presented. UPSCALE is an acronym for UK on PRACE (the Partnership for Advanced Computing in Europe)-weather-resolving Simulations of Climate for globAL Environmental risk. The UPSCALE experiment was performed in 2012 by NCAS (National Centre for Atmospheric Science)-Climate, at the University of Reading and the UK Met Office Hadley Centre. The study included a 25.6-year, five-member ensemble simulation of the HadGEM3 global atmosphere, at 25km resolution for present climate conditions. The initial conditions for the ensemble runs were taken from consecutive days of a test configuration. In the present paper, the emphasis is placed on the single climate run for a potential future climate scenario in the UPSCALE experiment dataset, using the Representation Concentrations Pathways (RCP) 8.5 climate change scenario. Firstly, some tests were performed to ensure that the results using only one instantiation of the current climate dataset are as robust as possible within the constraints of the available data. In order to achieve this, an artificial time series over a longer sampling period was created. Then, it was shown that these longer time series provided almost the same results than the short ones, thus leading to the argument that the short time series is sufficient to capture the climate. Finally, with the confidence that one instantiation is sufficient, the future climate dataset was analysed to provide, for the first time, a projection of future changes in wind power resources using the UPSCALE dataset. It is hoped that this, in turn, will provide

  16. Regional climate change over South Korea projected by the HadGEM2-AO and WRF model chain under RCP emission scenarios

    Science.gov (United States)

    Ahn, Joong-Bae; Im, Eun-Soon; Jo, Sera

    2017-04-01

    This study assesses the regional climate projection newly projected within the framework of the national downscaling project in South Korea. The fine-scale climate information (12.5 km) is produced by dynamical downscaling of the HadGEM2-AO global projections forced by the representative concentration pathway (RCP4.5 and 8.5) scenarios using the Weather Research and Forecasting (WRF) modeling system. Changes in temperature and precipitation in terms of long-term trends, daily characteristics and extremes are presented by comparing two 30 yr periods (2041-2070 vs. 2071-2100). The temperature increase presents a relevant trend, but the degree of warming varies in different periods and emission scenarios. While the temperature distribution from the RCP8.5 projection is continuously shifted toward warmer conditions by the end of the 21st century, the RCP4.5 projection appears to stabilize warming in accordance with emission forcing. This shift in distribution directly affects the magnitude of extremes, which enhances extreme hot days but reduces extreme cold days. Precipitation changes, however, do not respond monotonically to emission forcing, as they exhibit less sensitivity to different emission scenarios. An enhancement of high intensity precipitation and a reduction of weak intensity precipitation are discernible, implying an intensified hydrologic cycle. Changes in return levels of annual maximum precipitation suggest an increased probability of extreme precipitation with 20 yr and 50 yr return periods. Acknowledgement : This work was funded by the Korea Meteorological Administration Research and Development Program under grant KMIPA 2015-2081

  17. Impacts of changes in climate and land use/land cover under IPCC RCP scenarios on streamflow in the Hoeya River Basin, Korea.

    Science.gov (United States)

    Kim, Jinsoo; Choi, Jisun; Choi, Chuluong; Park, Soyoung

    2013-05-01

    This study examined the separate and combined impacts of future changes in climate and land use/land cover (LULC) on streamflow in the Hoeya River Basin, South Korea, using the representative concentration pathway (RCP) 4.5 and 8.5 scenarios of the Fifth Assessment Report (AR5) of the Intergovernmental Panel on Climate Change (IPCC). First, a LULC change model was developed using RCP 4.5 and RCP 8.5 storylines and logistic regression. Three scenarios (climate change only, LULC change only, and climate and LULC change combined) were established, and the streamflow in future periods under these scenarios was simulated by the Soil and Water Assessment Tool (SWAT) model. Each scenario showed distinct seasonal variations in streamflow. Under climate change only, streamflow increased in spring and winter but decreased in summer and autumn, whereas LULC change increased high flow during wet periods but decreased low flow in dry periods. Although the LULC change had less effect than climate change on the changes in streamflow, the effect of LULC change on streamflow was significant. The result for the combined scenario was similar to that of the climate change only scenario, but with larger seasonal changes in streamflow. Although the effects of LULC change were smaller than those caused by climate change, LULC changes may heighten the problems of increased seasonal variability in streamflow caused by climate change. The results obtained in this study provide further insight into the availability of future streamflow and can aid in water resource management planning in the study area. Copyright © 2013 Elsevier B.V. All rights reserved.

  18. Analysis of occlusal contacts in different types of prosthodontic appliances, Eichner classifications, presence RCP-ICP slide and the type of occlusion.

    Science.gov (United States)

    Stipetić, J; Celebić, A; Baucić, I; Lazić, B; Komar, D; Bratolić, V; Catić, A; Stefancić, S

    2001-06-01

    The aim of the study was to determine the number and the type of the occlusal contacts (strong, weak) with respect to the type of the prosthodontic appliance (fixed, removable, combined fixed-removable) and with respect to the Eichner classification in patients with their appliances being in a good function for a long time. The aim of the study was also to determine the number and the type of the occlusal contacts (strong, weak) with respect to the type of occlusion (canine guided, group function or balanced) and the presence of the RCP-ICP slide. A total of 440 patients with different types of prosthodontic appliances were examined for the antagonistic occlusal contacts using occlusal strips of 11 microm and 50 microm. The average number of occlusal contacts was 10.5 for the upper and 10.46 for the lower posterior teeth, approximately 5 on each side of the tooth arch. The results of the study suggest that the biggest number of occlusal contacts were recorded for the small span fixed appliances (2 on average), the greater span fixed and fixed-removable prosthodontic appliances exhibited 1.6 occlusal contacts, and the removable complete denture exhibited 1.2 contact per the tooth in the posterior region. The number of the hard occlusal contacts was significantly greater in fixed and fixed-removable prosthodontic appliances in comparison with the complete dentures (p 0.05). The overall number of the occlusal contacts, as well as the number of the hard occlusal contacts was significantly greater in the Eichner class I cases (p 0.05). There was no significant difference in the number of occlusal contacts between the appliances with RCP-ICP slide and where ICP and RCP corresponded (p > 0.05).

  19. From land use to land cover: Restoring the afforestation signal in a coupled integrated assessment - earth system model and the implications for CMIP5 RCP simulations

    Energy Technology Data Exchange (ETDEWEB)

    Di Vittorio, Alan V. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Chini, Louise M. [Univ. of Maryland, College Park, MD (United States); Bond-Lamberty, Benjamin [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Mao, Jiafu [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Shi, Xiaoying [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Truesdale, John E. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Craig, Anthony P. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Calvin, Katherine V. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Jones, Andrew D. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Collins, William D. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Edmonds, James A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Hurtt, George [Univ. of Maryland, College Park, MD (United States); Thornton, Peter E. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Thomson, Allison M. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2014-11-27

    Climate projections depend on scenarios of fossil fuel emissions and land use change, and the IPCC AR5 parallel process assumes consistent climate scenarios across Integrated Assessment and Earth System Models (IAMs and ESMs). To facilitate consistency, CMIP5 used a novel land use harmonization to provide ESMs with seamless, 1500-2100 land use trajectories generated by historical data and four IAMs. However, we have identified and partially addressed a major gap in the CMIP5 land coupling design. The CMIP5 Community ESM (CESM) global afforestation is only 22% of RCP4.5 afforestation from 2005 to 2100. Likewise, only 17% of the Global Change Assessment Model’s (GCAM’s) 2040 RCP4.5 afforestation signal, and none of the pasture loss, were transmitted to CESM within a newly integrated model. This is a critical problem because afforestation is necessary for achieving the RCP4.5 climate stabilization. We attempted to rectify this problem by modifying only the ESM component of the integrated model, enabling CESM to simulate 66% of GCAM’s afforestation in 2040, and 94% of GCAM’s pasture loss as grassland and shrubland losses. This additional afforestation increases vegetation carbon gain by 19 PgC and decreases atmospheric CO2 gain by 8 ppmv from 2005 to 2040, implying different climate scenarios between CMIP5 GCAM and CESM. Similar inconsistencies likely exist in other CMIP5 model results, primarily because land cover information is not shared between models, with possible contributions from afforestation exceeding model-specific, potentially viable forest area. Further work to harmonize land cover among models will be required to adequately rectify this problem.

  20. Steric Sea Level Change in Twentieth Century Historical Climate Simulation and IPCC-RCP8.5 Scenario Projection: A Comparison of Two Versions of FGOALS Model

    Institute of Scientific and Technical Information of China (English)

    DONG Lu; ZHOU Tianjun

    2013-01-01

    To reveal the steric sea level change in 20th century historical climate simulations and future climate change projections under the IPCC's Representative Concentration Pathway 8.5 (RCP8.5) scenario,the results of two versions of LASG/IAP's Flexible Global Ocean-Atmosphere-Land System model (FGOALS) are analyzed.Both models reasonably reproduce the mean dynamic sea level features,with a spatial pattern correlation coefficient of 0.97 with the observation.Characteristics of steric sea level changes in the 20th century historical climate simulations and RCP8.5 scenario projections are investigated.The results show that,in the 20th century,negative trends covered most parts of the global ocean.Under the RCP8.5 scenario,global-averaged steric sea level exhibits a pronounced rising trend throughout the 21st century and the general rising trend appears in most parts of the global ocean.The magnitude of the changes in the 21st century is much larger than that in the 20th century.By the year 2100,the global-averaged steric sea level anomaly is 18 cm and 10 cm relative to the year 1850 in the second spectral version of FGOALS (FGOALS-s2) and the second grid-point version of FGOALS (FGOALS-g2),respectively.The separate contribution of the thermosteric and halosteric components from various ocean layers is further evaluated.In the 20th century,the steric sea level changes in FGOALS-s2 (FGOALS-g2) are largely attributed to the thermosteric (halosteric) component relative to the pre-industrial control run.In contrast,in the 21st century,the thermosteric component,mainly from the upper 1000 m,dominates the steric sea level change in both models under the RCP8.5 scenario.In addition,the steric sea level change in the marginal sea of China is attributed to the thermosteric component.

  1. Measuring Progress on the Control of Porcine Reproductive and Respiratory Syndrome (PRRS at a Regional Level: The Minnesota N212 Regional Control Project (Rcp as a Working Example.

    Directory of Open Access Journals (Sweden)

    Pablo Valdes-Donoso

    Full Text Available Due to the highly transmissible nature of porcine reproductive and respiratory syndrome (PRRS, implementation of regional programs to control the disease may be critical. Because PRRS is not reported in the US, numerous voluntary regional control projects (RCPs have been established. However, the effect of RCPs on PRRS control has not been assessed yet. This study aims to quantify the extent to which RCPs contribute to PRRS control by proposing a methodological framework to evaluate the progress of RCPs. Information collected between July 2012 and June 2015 from the Minnesota Voluntary Regional PRRS Elimination Project (RCP-N212 was used. Demography of premises (e.g. composition of farms with sows = SS and without sows = NSS was assessed by a repeated analysis of variance. By using general linear mixed-effects models, active participation of farms enrolled in the RCP-N212, defined as the decision to share (or not to share PRRS status, was evaluated and used as a predictor, along with other variables, to assess the PRRS trend over time. Additionally, spatial and temporal patterns of farmers' participation and the disease dynamics were investigated. The number of farms enrolled in RCP-N212 and its geographical coverage increased, but the proportion of SS and NSS did not vary significantly over time. A significant increasing (p<0.001 trend in farmers' decision to share PRRS status was observed, but with NSS producers less willing to report and a large variability between counties. The incidence of PRRS significantly (p<0.001 decreased, showing a negative correlation between degree of participation and occurrence of PRRS (p<0.001 and a positive correlation with farm density at the county level (p = 0.02. Despite a noted decrease in PRRS, significant spatio-temporal patterns of incidence of the disease over 3-weeks and 3-kms during the entire study period were identified. This study established a systematic approach to quantify the effect of RCPs on

  2. MP-RCP:ACHIEVING FAST RECOVERY OF iBGP USING ROUTE CONTROL PLATFORM%MP-RCP:基于RCP的快速恢复iBGP协议

    Institute of Scientific and Technical Information of China (English)

    程柏林; 胡乔林; 陈新; 吕树君

    2014-01-01

    Mechanism of iBGP single best path propagation may prevent the router from acquiring diverse paths,which results in connection disruption and inter-domain churn in the scene of transient link.MP-RCP optimises the route control platform (RCP)to reduce the session numbers and configuration complexity,makes use of global visible routes to compute and disseminate multiple paths for each router.When a router detects the failure in best path,the router encapsulates the packets and forwards them to the backup path to avoid the connection disruption.MP-RCP also separates the failure detection and processing in order to prevent the frequent convergence triggered by transient failures,which reduces the inter-domain disturbance.It is proved through Abilene topology and the experiment of topology generation that the MP-RCP structure reduces the forwarding disruption and inter-domain churn effectively.%iBGP 传播单条最佳路径机制不能保证路由器获得多样性路径,造成链路瞬时故障场景下连接中断及域间扰动。MP-RCP(MultiPath Route Control Platform)优化路由控制平台RCP以降低会话数量及配置复杂性,利用全局可视路由对每台路由器计算、分发多路径。当路由器检测到最佳路径失效后,将报文封装转发到备份路径中避免连接中断;MP-RCP中将失效检测、处理进行分离以避免瞬时故障引起的频繁收敛,从而降低了域间扰动。通过使用Abilene拓扑以及生成拓扑的实验证明了MP-RCP结构能有效避免转发中断和域间扰动。

  3. Monte Carlo modelling projects the loss of most land-terminating glaciers on Svalbard in the 21st century under RCP 8.5 forcing

    Science.gov (United States)

    Möller, Marco; Navarro, Francisco; Martín-Español, Alba

    2016-09-01

    The high Arctic archipelagos around the globe are among the most strongly glacierized landscapes on Earth apart from the Greenland and Antarctic ice sheets. Over the past decades, the mass losses from land ice in the high Arctic regions have contributed substantially to global sea level rise. Among these regions, the archipelago of Svalbard showed the smallest mass losses. However, this could change in the coming decades, as Svalbard is expected to be exposed to strong climate warming over the 21st century. Here we present extensive Monte Carlo simulations of the future ice-mass evolution of 29 individual land-terminating glaciers on the Svalbard archipelago under an RCP 8.5 climate forcing. An extrapolation of the 29 sample glaciers to all land-terminating glaciers of the archipelago suggests an almost complete deglaciation of the region by 2100. Under RCP 8.5, 98% of the land-terminating glaciers will have declined to less than one tenth of their initial size, resulting in a loss of 7392 ± 2481 km2 of ice coverage.

  4. Imunodiagnóstico da leptospirose humana através do teste ELISA-IgM, empregando-se diferentes preparações antigênicas a partir de sorotipos prevalentes de Leptospira interrogans Immunodiagnostic of human leptospirosis by ELISA-IgM, employing: different antigenic preparations as from prevalent serovars of Leptospira interrogans

    Directory of Open Access Journals (Sweden)

    Marcos Vinicius da Silva

    1990-08-01

    Full Text Available Realizou-se estudo comparativo de diferentes sorotipos de Leptospira interrogans utilizados na preparação de antígenos empregados no teste ELISA, para a detecção de anticorpos da classe IgM, em amostras de soro na fase precoce e tardia da leptospirose humana. Foram utilizados dez sorotipos, escolhidos entre os que apresentaram maior reatividade na soroaglutinação microscópica (SAM, na cidade de São Paulo. Os cinco sorotipos que apresentaram melhores resultados individualmente no teste ELISA-IgM (canicola, hebdomadis, icterohaemorrhagiae, cynopteri e brasiliensis, foram também estudados em mistura antigênica. Os antígenos não tratados apresentaram maior reatividade do que os antígenos tratados com Triton X - 100 (4% à temperatura de 50ºC, durante 4 horas. O teste ELISA-IgM empregando os sorotipos não tratados, isoladamente, e em mistura antigênica, mostrou-se altamente sensível, podendo ser empregado como teste de triagem para o diagnóstico precoce da leptospirose humana. Outra aplicação do teste é permitir a detecção do início de situações epidêmicas ou de surtos, possibilitando acionar medidas de vigilância epidemiológica.A comparative study among different serovars of Leptospira interrogans was performed in order to prepare antigens to detect IgM antibodies by ELISA in early and late phase of human leptospirosis. Ten serovars were chosen among the most prevalent detected by microscopic seroagglutination (SAM in São Paulo city. Using ELISA-IgM five of them showed better results (canicola, hebdomadis, icterohaemorrhagiae, cynopteri and brasiliensis. These ones were also studied in a pool. The non-treated antigens showed higher reactivity than the Triton X-100 (4%/50ºC/4h. ELISA-IgM using individually or pool of non treated antigens proved to be reliable with high sensitivity and should be used for an earlier diagnosis of leptospirosis, as a trial test. Faster diagnostic elucidation can be useful to detect

  5. SOROPREVALÊNCIA DE ANTICORPOS ANTI-Neospora caninum E ANTI-Leptospira interrogans EM DUAS PROPRIEDADES DE VACAS LEITEIRAS COM RELATOS DE PREJUÍZOS REPRODUTIVOS NOMUNICÍPIO DE GOIANDIRA, GOIÁS

    Directory of Open Access Journals (Sweden)

    Rafael Quirino Moreira

    2010-06-01

    Full Text Available The aim of this study was to determine the seroprevalence ofantibodies anti-Neospora caninum and anti-Leptospira interrogansin dairy cows from Goiandira, Goiás State, Brazil. Serum samplesof 53 dairy cows from two herds were assayed for anti-N. caninumantibodies using an indirect immunofluorescent antibody test (IFATand microscopic-agglutination technique (MAT for L. interrogansagglutinins. Sera reacting at dilutions ≥ 1:50 and ≥ 1:100 wereconsidered positive for N. caninum and L. interrogans antibodiesrespectively. It was found that 35.9% (14 cows of the samplesfrom Farm 1 and 64.3% (9 cows from Farm 2 were positive forN. caninum. For L. interrogans, 87.2% (34 cows from Farm 1 and64.3% (9 cows from Farm 2 were positive. The serovars foundedwere: wolffi (21.74%; hardjo (18.11%; bratislava (14.49%;australis (12.32%; tarassovi (12.32%; icterohaemorrhagiae(7.25%; sentot (5.80%, grippotyphosa (5.07%; patoc (2.17%;cynoptero (0.72%. According to the results, it was possible toconclude that neosporosis and leptospirosis are endemic in dairycows and may also be a significant animal and human healthproblem in Goiandira, GO, Brazil.

  6. RECURSOS NO CONVENCIONALES SUSCEPTIBLES DE SER EXPLOTADOS MEDIANTE FRACKING

    OpenAIRE

    Jódar Abellán, Antonio

    2014-01-01

    En el presente trabajo se analizan en detalle los recursos de origen no convencional existentes en el contexto internacional, europeo, nacional y regional, que pueden ser explotados mediante fracking. Así mismo, se mencionará someramente la legislación aplicable a la fracturación hidráulica y los impactos, tanto beneficiosos como perjudiciales, que ésta puede acarrear. El estudio finaliza con una propuesta de posibles lugares en la Península Ibérica donde dicha técnica reporte mayores benefic...

  7. Research on application system based on J2EE and Eclipse RCP%基于J2EE与Eclipse RCP的应用系统研究

    Institute of Scientific and Technical Information of China (English)

    郭盈; 耿莉

    2008-01-01

    Eclipse RCP (Rich Client Platform,富客户端平台)是基于Eclipse项目的一个开发富客户端应用程序的框架(如图1所示),它提供了一个通用的Eclipse工作台,允许开发人员扩建和构造强大的、跨平台的应用程序。开发人员可以定义应用程序外观、商标和其他Eclipse基础组件等,实现自己的桌面应用程序。目前,使用RCP开发的产品很多,

  8. en sistemas modelo de gelatina mediante SPME-DED

    Directory of Open Access Journals (Sweden)

    J. Ruiz Carrascal

    2005-01-01

    Full Text Available Se llevó a cabo la determinación de 16 hidrocarburos aromáticos policíclicos y 9 nitrosaminas en sistemas modelo de gelatina (20% w/v mediante microextración en fase sólida acoplada a un dispositivo de extracción directa y posterior análisis mediante cromatografía-gaseosa-espectrometría de masas. La extracción se llevo a cabo a 25ºC. Se extrajeron la totalidad de las nitrosaminas y 9 de los 16 hidrocarburos aromáticos policíclicos. Se evaluaron la reproducibilidad, linealidad de respuesta y límite de detección de 3 tipos de fibras estacionarias para cada tipo de compuesto. Se seleccionaron las fibras de polidimetilsiloxano 100μm y carboxen/polidimetilsiloxano 85μm, para los hidrocarburos aromáticos policiclicos y nitrosaminas, respectivamente. La microextración en fase sólida acoplada a un dispositivo de extracción directa surge como una técnica interesante para la monitorización preliminar de la presencia de estos compuestos tóxicos en alimentos sólidos, sin necesidad de toma de muestras y sin deteriorar el producto

  9. Infecção experimental em suínos jovens com Leptospira interrogans sorovar wolffi: determinação de parâmetros bioquímicos Experimental infection by Leptospira interrogans serovar wolffi in young pigs: determination of biochemical parameters

    Directory of Open Access Journals (Sweden)

    José Carlos Rende

    2007-04-01

    Full Text Available Um estudo sobre infecção experimental foi realizado em oito suínos, com idade média de 90 dias, machos castrados, da raça Wessex, e distribuídos em dois grupos de quatro suínos cada. Durante 36 dias, foram analisadas as alterações bioquímicas nos soros dos suínos dos dois grupos. O Grupo I foi mantido como testemunho e recebeu 5,0mL de solução fisiológica estéril por via intravenosa (veia cava craniana e, no Grupo II, os suínos foram inoculados pela mesma via com 5,0mL de cultura de Leptospira interrogans sorovar wolffi , amostra L-10 selvagem isolada de tatu (Dasypus novemcinctus, contendo 1,0 x 10(8 leptospiras/mL. A partir do terceiro dia após a inoculação e em intervalos de 72 horas até o décimo oitavo dia, foram feitas coletas de sangue, sem anticoagulante, dos animais inoculados e testemunhas. Os parâmetros bioquímicos analisados foram: bilirrubina total, direta e indireta, ácidos graxos, glicose e proteínas plasmáticas. Foi detectado um aumento da bilirrubina direta no terceiro dia e um aumento no sexto dia da bilirrubina total e indireta após a inoculação. As dosagens de glicose, ácidos graxos e proteínas plasmáticas apresentaram uma diminuição a partir do terceiro dia da inoculação. Com os resultados obtidos, pode-se concluir que o aumento das taxas de bilirrubinas levam a uma definição de um diagnóstico de hemólise aguda, e que a hipoglicemia, a hipolipidemia e a hipoproteinemia podem estar relacionadas com lesões hepáticas e a uma septcemia.Todas as dosagens em todos os animais retornaram aos seus valores normais a partir do décimo quinto dia.Eight, 90 days old pigs, of the Wessex lineage all castrated male were used in experiment, divided into two groups of four animals. Biochemical alterations in the serum of the animals were analyzed in both groups during 36 days. Control (Group I received 5.0mL of a 0.9% sterile sodium chloride solution by intracranial vein injection; Group II animals

  10. Characterization of acetyl-CoA and propionyl-CoA carboxylases encoded by Leptospira interrogans serovar Lai: an initial biochemical study for leptospiral gluconeogenesis via anaplerotic CO2 assimilation

    Institute of Scientific and Technical Information of China (English)

    Nanqiu Peng; Yi Zhong; Qing Zhang; Mingyue Zheng; Wei Zhao; Hualiang Jiang; Chen Yang; Xiaokui Guo; Guoping Zhao

    2012-01-01

    Leptospira interrogans is the causative agent of leptospirosis.The in vitro growth of L.interrogans requires CO2 and a partial 3-hydroxypropionate pathway involving two acyl-CoA carboxylases was suggested by genomic analysis to assimilate CO2.Either set of the candidate genes heterologously co-expressed in Escherichia coli was able to demonstrate both acetyl-CoA carboxylase (ACC)and propionyl-CoA carboxylase (PCC) activities.The trisubunit holoenzyme (LA_2736-LA_2735 and LA_3803),although failed to be purified,was designated ACC based on its substrate preference toward acetyl-CoA.The partially purified bi-subunit holoenzyme (LA_2432-LA_2433) has a considerably higher activity against propionyi-CoA as the substrate than that of acetyl-CoA,and thus,designated PCC.Native polyacrylamide gel electrophoresis indicated that this PCC has a molecular mass of around 669 kDa,suggesting an α4β4 quaternary structure and both structural homology modeling and site-directed mutagenesis analysis of its carboxyltransferase subunit (LA_2433) indicated that the A431 residue located at the bottom of the putative substrate binding pocket may play an important role in substrate specificity determination.Both transcriptomic and proteomic data indicated that enzymes involved in the suggested partial 3-hydroxypropionate pathway were expressed in vivo in addition to ACC/PCC and the homologous genes in genomes of other Leptospira species were re-annotated accordingly.However,as the in vitro detected specific activity of ACC in the crude cell extract was too low to account for the growth of the bacterium in Ellinghausen-McCulloughJohnson-Harris minimal medium,further systematic analysis is required to unveil the mechanism of gluconeogenesis via anaplerotic CO2 assimilation in Leptospira species.

  11. MEDIANTE LA APLICACIÓN DEL ENSAYO FÉNIX.

    Directory of Open Access Journals (Sweden)

    Linna Marcela Neme Ardila

    2013-01-01

    Full Text Available Las mezclas asfálticas son el material más utilizado en la fabricación de pavimentos y los ensayos que permiten caracterizarlas son costosos y demorados. Por esta razón, mediante esta investigación se planteó establecer la viabilidad del uso del ensayo Fénix en mezclas asfálticas colombianas con granulometrías del Instituto de Desarrollo Urbano (IDU y del Instituto Nacional de Vías (INVIAS con diferentes características. El estudio inició con la fabricación de probetas Fénix con diferentes materiales (Agregados, asfaltos, asfaltita, pavimento asfalto reciclado (RAP, cal, cemento y su ejecución a 15 °C, una velocidad de 1 mm/min y la medición de los parámetros del ensayo. De los resultados obtenidos de resistencia a tracción, índice de rigidez a tracción e índice de energía, área elástica y área de fluencia, se estableció que el ensayo Fénix es un procedimiento eficaz, eficiente, económico, rápido y sencillo para determinar las propiedades mecánicas y dinámicas de las mezclas asfálticas estudiadas, especialmente mediante el análisis de los parámetros de las curvas carga-desplazamiento, irrelevantemente de la mezcla asfáltica fabricada.

  12. Future Projection of Storm Surge at Tokyo Bay under RCP 8.5 Scenario by Meteorological-Ocean-Tide Coupled Model

    Science.gov (United States)

    Iwamoto, T.; Nakamura, R.; Takagawa, T.; Shibayama, T.

    2016-12-01

    It is clearly valuable to accomplish well-reproduced storm surge model and conduct future projection for disaster prevention. In this study, the reproducibility of Meteorological-Ocean-Tide coupled model was validated by simulating typhoon Roke (2011) storm surge, which was recorded as the highest anomaly (119cm) at Tokyo tide station (JMA) in Tokyo Bay over the last 10 years. Furthermore, the future projection (2050) under global warming scenario (RCP8.5) was conducted. The coupled model was composed of 3 models; ARW-WRFV3 (Skamarock et al., 2008), FVCOM (Chen et al., 2011) and WXTide32. WRF firstly calculated downscaled meteorological field by using FiNal anaLysis (FNL) as initial/boundary (I/B) condition. In this calculation, single layer urban canopy model (Kusaka et al., 2001) and topography data from SRTM3 (90m mesh) and GSI (50m mesh) were applied. Then the output was used as I/B condition to FVCOM, which calculated storm surge. Finally tide level was calculated by adding storm surge to astronomical tide calculated by WXTide32. For 2050 case, sea surface temperature (SST) from 26 GCM under RCP8.5 was used for constructing pseudo global warming meteorological fields. In details, ensemble average of SST variation between 2006-2015 and 2041-2060 was added to FNL's SST by following Oya et al (2016). In this case, calculating astronomical tide is omitted due to the limitation of WXTide32. The reproduced result of typhoon Roke shows that the difference of maximum tide level (first peak) to the observation is less than 10cm, the difference of second peak is about 50cm. The future projection result shows that the increase of storm surge at Tokyo tide station is about 20cm and that at Funabashi is about 30cm. This intensification is mainly caused by wind speed increment, since the variation of low pressure due to higher SST is relatively small. Moreover, Funabashi is located in front of the open space at inner part of Tokyo Bay, Tokyo tide station is similar however

  13. Drivers of the tropospheric ozone budget throughout the 21st century under the medium-high climate scenario RCP 6.0

    Directory of Open Access Journals (Sweden)

    L. E. Revell

    2015-01-01

    Full Text Available Because tropospheric ozone is both a~greenhouse gas and harmful air pollutant, it is important to understand how anthropogenic activities may influence its abundance and distribution through the 21st century. Here, we present model simulations performed with the chemistry-climate model SOCOL, in which spatially disaggregated chemistry and transport tracers have been implemented in order to better understand the distribution and projected changes in tropospheric ozone. We examine the influences of ozone precursor emissions (nitrogen oxides (NOx, carbon monoxide (CO and volatile organic compounds (VOCs, climate change and stratospheric ozone recovery on the tropospheric ozone budget, in a~simulation following the climate scenario Representative Concentration Pathway (RCP 6.0. Changes in ozone precursor emissions have the largest effect, leading to a global-mean increase in tropospheric ozone which maximises in the early 21st century at 23%. The increase is most pronounced at northern midlatitudes, due to regional emission patterns: between 1990 and 2060, northern midlatitude tropospheric ozone remains at constantly large abundances: 31% larger than in 1960. Over this 70 year period, attempts to reduce emissions in Europe and North America do not have an effect on zonally-averaged northern midlatitude ozone because of increasing emissions from Asia, together with the longevity of ozone in the troposphere. A~simulation with fixed anthropogenic ozone precursor emissions of NOx, CO and non-methane VOCs at 1960 conditions shows a 6 % increase in global-mean tropospheric ozone, and an 11% increase at northern midlatitudes. This increase maximises in the 2080s, and is mostly caused by methane, which maximises in the 2080s following RCP 6.0, and plays an important role in controlling ozone directly, and indirectly through its influence on other VOCs and CO. Enhanced flux of ozone from the stratosphere to the troposphere as well as climate change

  14. AP1000核电站主泵的电气系统分析%Analysis of the RCP Electrical System of AP1000 Nuclear Power Plant

    Institute of Scientific and Technical Information of China (English)

    王宪坤

    2012-01-01

    AP1000 is the 3rd generation nuclear power plant imported from Westinghouse.It takes full advantage of passive techniques on the basis of safety coefficient enhancement,and it cancels the nuclear-grade active devices such as emergency diesel generator,decreases the operations by operators,and therefore decreases the human factor influences.It uses the minimum systems and equipment.and therefore simplifies the configuration.It adopts the advanced equipment that with long maintenance cycle or maintenance free,it decreases the interfaces of systems and equipment,enhances the safety response ability of the power plant to serious accidents or natural disasters.The RCP system is a key item for importing this technology due to its importance and particularity.It's to understand the configuration and special structure of the electric equipment by analyzing the function of RCP and the principle of electric system.%AP1000核电站在提高安全系数的基础上,充分利用大量的非能动技术,取消了应急柴油发电机等核级能动设备,减少了操作员的干预动作,降低了人因因素的影响。使用尽可能少的系统和设备,使得布置简化;采用了维修周期更长甚至免检修的先进设备,减少系统及设备之间的接口,提高了核电厂自身应对各种严重事故或自然灾害的安全响应能力。其中主泵系统由于它的重要性和特殊性,也是技术引进的重点项目;文章通过对主泵的作用和电气系统原理分析,阐述了电气设备的配置及特殊结构。

  15. Projections of Rainfall and Surface Temperature from CMIP5 Models under RCP4.5 and 8.5 over BIMSTEC Countries

    Science.gov (United States)

    Charan Pattnayak, Kanhu; Kar, Sarat Chandra; Kumari Pattnayak, Rashmita

    2015-04-01

    Rainfall and surface temperature are the most important climatic variables in the context of climate change. Thus, these variables simulated from fifth phase of the Climate Model Inter-comparison Project (CMIP5) models have been compared against Climatic Research Unit (CRU) observed data and projected for the twenty first century under the Representative Concentration Pathways (RCPs) 4.5 and 8.5 emission scenarios. Results for the seven countries under Bay of Bengal Initiative for Multi-Sectoral Technical and Economic Cooperation (BIMSTEC) such as Bangladesh, Bhutan, India, Myanmar, Nepal, Sri Lanka and Thailand have been examined. Six CMIP5 models namely GFDL-CM3, GFDL-ESM2M, GFDL-ESM2G, HadGEM2-AO, HadGEM2-CC and HadGEM2-ES have been chosen for this study. The study period has been considered is from 1861 to 2100. From this period, initial 145 years i.e. 1861 to 2005 is reference or historical period and the later 95 years i.e. 2005 to 2100 is projected period. The climate change in the projected period has been examined with respect to the reference period. In order to validate the models, the mean annual rainfall and temperature has been compared with CRU over the reference period 1901 to 2005. Comparison reveals that most of the models are able to capture the spatial distribution of rainfall and temperature over most of the regions of BIMSTEC countries. Therefore these model data can be used to study the future changes in the 21st Century. Four out six models shows that the rainfall over Central and North India, Thailand and eastern part of Myanmar shows decreasing trend and Bangladesh, Bhutan, Nepal and Sri Lanka shows an increasing trend in both RCP 4.5 and 8.5 scenarios. In case of temperature, all of the models show an increasing trend over all the BIMSTEC countries in both scenarios, however, the rate of increase is relatively less over Sri Lanka than the other countries. Annual cycles of rainfall and temperature over Bangladesh, Myanmar and Thailand

  16. Identificação da Leptospira interrogans sorovar pomona em camundongos geneticamente selecionados, para a alta e baixa produção de anticorpos, através da técnica de imunoperoxidase em tecido renal e isolamento bacteriano em meio de Fletcher Identification of Leptospira interrogans serovar pomona in mice genetically selected for high and low antibody production, by means of the immunoperoxidase technique in renal tissue and bacterial isolation in Fletcher medium

    Directory of Open Access Journals (Sweden)

    Maria Cristina Santos Haanwinckel

    2007-12-01

    Full Text Available O presente trabalho teve por objetivo identificar a presença da Leptospira interrogans sorovar pomona em camundongos geneticamente selecionados para a alta e baixa resposta a anticorpos. Todos os animais foram submetidos ao isolamento bacteriano, imunohistoquímica (imunoperoxidase em cortes de tecido renal e coloração através da hematoxilina-eosina. A técnica de imunoperoxidase apresentou-se pouco mais sensível em relação ao cultivo, entretanto, ambas foram bons parâmetros de identificação do agente. Presença de lesões renais mais intensas ocorreram em períodos em que houve maior número de bactérias isoladas em meio de cultivo. Camundongos da linhagem HIV-A conseguiram eliminar as leptospiras com maior eficiência e rapidez em relação as linhagem LIV-A, entretanto o estudo demonstrou que ambas linhagens da seleção IV-A foram eficientes em controlar o processo infeccioso.The present work had the objective of identifying the presence of Leptospira interrogans serovar pomona in mice that had been genetically selected for high and low response to antibodies. All the animals were subjected to bacterial isolation, immunohistochemical analysis (immunoperoxidase in renal tissue sections and hematoxylin-eosin staining. The immunoperoxidase technique was little more sensitive than culturing, but both were good parameters for agent identification. More severe renal lesions were present at times when there were greater numbers of bacteria isolated in culture medium. Mice of the lineage HIV-A were able to eliminate the Leptospira more efficiently and faster than the lineage LIV-A could. However, the study demonstrated that both lineages of the IV-A selection were efficient in controlling the infectious process.

  17. Structure of recombinant capsids formed by the beta-annulus deletion mutant -- rCP (Delta48-59) of Sesbania mosaic virus.

    Science.gov (United States)

    Pappachan, Anju; Subashchandrabose, Chinnathambi; Satheshkumar, P S; Savithri, H S; Murthy, M R N

    2008-05-25

    A unique feature of several T=3 icosahedral viruses is the presence of a structure called the beta-annulus formed by extensive hydrogen bonding between protein subunits related by icosahedral three-fold axis of symmetry. This unique structure has been suggested as a molecular switch that determines the T=3 capsid assembly. In order to examine the importance of the beta-annulus, a deletion mutant of Sesbania mosaic virus coat protein in which residues 48-59 involved in the formation of the beta-annulus were deleted retaining the rest of the residues in the amino terminal segment (rCP (Delta48-59)) was constructed. When expressed in Escherichia coli, the mutant protein assembled into virus like particles of sizes close to that of the wild type virus particles. The purified capsids were crystallized and their three dimensional structure was determined at 3.6 A resolution by X-ray crystallography. The mutant capsid structure closely resembled that of the native virus particles. However, surprisingly, the structure revealed that the assembly of the particles has proceeded without the formation of the beta-annulus. Therefore, the beta-annulus is not essential for T=3 capsid assembly as speculated earlier and may be formed as a consequence of the particle assembly. This is the first structural demonstration that the virus particle morphology with and without the beta-annulus could be closely similar.

  18. Extensive nonhomologous meiotic synapsis between normal chromosome axes of an rcp(3;6)(p14;q21) translocation in a hairless Mexican boar.

    Science.gov (United States)

    Villagómez, D A F; Ayala-Valdovinos, M A; Galindo-García, J; Sánchez-Chipres, D R; Mora-Galindo, J; Taylor-Preciado, J J

    2008-01-01

    Due to its low fertility, expressed as small litter size, a Mexican hairless boar was subjected to cytogenetic investigation. Analysis of G-banded mitotic chromosomes revealed a reciprocal chromosome translocation, rcp(3;6) (p14;q21). Synaptonemal complex analysis showed a regular pairing behavior of the translocation chromosome axes, always resulting in a quadrivalent configuration. However, due to extensive nonhomologous pairing between the axes of nonderivative chromosomes 3 and 6, the quadrivalent mostly had an asymmetrical cross-shaped morphology. The nonhomologous pairing occurred not only at mid and late pachytene, but also at the earliest stage of pachytene. It seems that early pachytene heterosynapsis is a common phenomenon in the pairing behavior of pig reciprocal translocations. Therefore, heterosynapsis may reduce apoptosis of germ cells due to partial absence of homologous synapsis during the pairing phase of meiosis. The frequency of spermatocytes showing quadrivalent configurations with unpaired axial segments apparently did not affect germ cell progression in the boar, since fairly normal testicular histology was noticed. Copyright 2008 S. Karger AG, Basel.

  19. Thermal simulation for the chip model based on RCP package%基于RCP封装的芯片模型电热模拟

    Institute of Scientific and Technical Information of China (English)

    张路

    2015-01-01

    基于集成电路重分布封装技术(redistributed chip packaging,RCP)的发展,通过提取芯片封装体的具体参数,建立并优化了RCP芯片的热学模型.采用有限元的方法计算了该模型在一定热耗散功率下,施加不同风速条件时的温度分布情况,结果表明:强制对流条件的施加显著增强了RCP芯片封装体的散热能力,4m/s的风速可使其系统热阻降低58%,但是随着风速的增大,其影响不断减弱.所得出的具体风速与芯片结温的关系,可为RCP封装技术的散热设计提供有价值的参考.

  20. Future PMPs Estimation in Korea under AR5 RCP 8.5 Climate Change Scenario: Focus on Dew Point Temperature Change

    Science.gov (United States)

    Okjeong, Lee; Sangdan, Kim

    2016-04-01

    According to future climate change scenarios, future temperature is expected to increase gradually. Therefore, it is necessary to reflect the effects of these climate changes to predict Probable Maximum Precipitations (PMPs). In this presentation, PMPs will be estimated with future dew point temperature change. After selecting 174 major storm events from 1981 to 2005, new PMPs will be proposed with respect to storm areas (25, 100, 225, 400, 900, 2,025, 4,900, 10,000 and 19,600 km2) and storm durations (1, 2, 4, 6, 8, 12, 18, 24, 48 and 72 hours) using the Korea hydro-meteorological method. Also, orographic transposition factor will be applied in place of the conventional terrain impact factor which has been used in previous Korean PMPs estimation reports. After estimating dew point temperature using future temperature and representative humidity information under the Korea Meteorological Administration AR5 RCP 8.5, changes in the PMPs under dew point temperature change will be investigated by comparison with present and future PMPs. This research was supported by a grant(14AWMP-B082564-01) from Advanced Water Management Research Program funded by Ministry of Land, Infrastructure and Transport of Korean government.

  1. Research on the Geological Monitoring Data Visualization Application Based on Eclipse RCP framework%基于Eclipse RCP框架的地质监测数据可视化应用研究

    Institute of Scientific and Technical Information of China (English)

    杨攀

    2015-01-01

    基于Eclilpse RCP的可视化技术和面向服务的SOA架构,分析了传统地质灾害监测数据的特点,针对地质灾害监测数据可视化的需求,结合Eclipse RCP的优点,构建了一种地质灾害监测数据的集成框架,实现了地质灾害监测数据的可视化显示。%Eclipse RCP technology and service-oriented architecture (SOA) software mode are used to analyze characteristics of the geological disaster data. Based on geological disaster monitoring data visualization need, combing with the advantages of the Eclipse RCP, this paper constructs an integrated framework of the geological disaster monitoring data and realizes the visualization of geological disaster monitoring data.

  2. Muestras y representatividad en vigilancia epidemiologica mediante sitios centinelas

    Directory of Open Access Journals (Sweden)

    Samaja Juan

    1996-01-01

    Full Text Available El artículo sostiene que las exigencias técnicas del muestreo para la vigilancia epidemiológica, exigen una revisión profunda de importantes conceptos de la Teoría de la Salud. En particular, es necesario hacer énfasis en las condiciones de vida, y, más específicamente, en los ambientes o contextos en que se desarrollan los procesos reproductivos de la vida social. Pero ambos campos temáticos exigen potenciar el acceso a datos más ricos que los que aportan las fuentes tradicionales. Este enfoque de la "vigilancia epidemiológica" exige una revisión de los tipos de muestras, y esto implica revisar las interpretaciones dominantes sobre los fundamentos lógicos de las inferencias a partir de muestras. Se torna necesario dejar atrás las muestras estadísticas (aún las estratificadas y promover procedimientos del tipo de los "sitios centinelas". Esta técnica, aplicada originariamente en sociedades con sistemas estadísticos deficitarios, puede desarrollarse para constituirse en un complemento substancial del monitoreo de condiciones de vida incluso en sociedades con buenos sistemas de información. El artículo propone transformar el concepto de "sitio centinela" incorporandole el requisito de la "representatividad cualitativa" mediante muestreos finalísticos sustentados en tipologías previas de las unidades espacio-poblacionales.

  3. Muestras y representatividad en vigilancia epidemiologica mediante sitios centinelas

    Directory of Open Access Journals (Sweden)

    Juan Samaja

    Full Text Available El artículo sostiene que las exigencias técnicas del muestreo para la vigilancia epidemiológica, exigen una revisión profunda de importantes conceptos de la Teoría de la Salud. En particular, es necesario hacer énfasis en las condiciones de vida, y, más específicamente, en los ambientes o contextos en que se desarrollan los procesos reproductivos de la vida social. Pero ambos campos temáticos exigen potenciar el acceso a datos más ricos que los que aportan las fuentes tradicionales. Este enfoque de la "vigilancia epidemiológica" exige una revisión de los tipos de muestras, y esto implica revisar las interpretaciones dominantes sobre los fundamentos lógicos de las inferencias a partir de muestras. Se torna necesario dejar atrás las muestras estadísticas (aún las estratificadas y promover procedimientos del tipo de los "sitios centinelas". Esta técnica, aplicada originariamente en sociedades con sistemas estadísticos deficitarios, puede desarrollarse para constituirse en un complemento substancial del monitoreo de condiciones de vida incluso en sociedades con buenos sistemas de información. El artículo propone transformar el concepto de "sitio centinela" incorporandole el requisito de la "representatividad cualitativa" mediante muestreos finalísticos sustentados en tipologías previas de las unidades espacio-poblacionales.

  4. CAPTURA DE CO2 MEDIANTE TRANSPORTADORES SÓLIDOS

    Directory of Open Access Journals (Sweden)

    Carmen Forero

    2011-01-01

    Full Text Available La evaluación de transportadores de oxígeno (TO, basados en CuO y NiO sobre Al2O3 y preparados por impregnación, se llevó a cabo en una planta piloto de dos lechos fluidizados interconectados de 500 Wte, donde se utilizaron tanto metano como gas de síntesis como gas combustible. Además, se estudió el efecto de diferentes impurezas presentes en el gas combustible como azufre o hidrocarburos ligeros en la eficacia de combustión del proceso y en el comportamiento de los TO. Los resultados obtenidos mostraron que ambos TO son adecuados para la captura de CO2 mediante transportadores sólidos de oxígeno en el proceso de combustión de metano, gas de síntesis o metano con impurezas como hidrocarburos ligeros o azufre en el gas.

  5. Análisis de varistores mediante STM y STS

    Directory of Open Access Journals (Sweden)

    Castro, M. S.

    2002-02-01

    Full Text Available ZnO-Varistors have been studied by scanning tunnelling microscopy and spectroscopy. Tunnelling current-voltage characteristics indicate that grains present a rectifying behaviour. Since the prebreakdown zone is controlled by Schottky barriers formed by intergranular states, current density vs. temperature curves were fitted considering the tunnelling current through these barriers. Then, the Schottky barrier heights determined using the model are compared with the barrier heights obtained from the scanning tunnelling spectroscopy analysis in the grain boundary.

    Varistores de ZnO fueron estudiados mediante el empleo de microscopía y espectroscopía de efecto túnel. Las curvas de corriente túnel vs. tensión indican que las muestras presentan una conducta rectificante. Dado que la conducción en la zona de pre-ruptura es controlada por la formación de barreras de Schottky en los bordes de grano debidas a la presencia de estados intergranulares, las curvas de densidad de corriente vs. temperatura fueron ajustadas considerando la corriente túnel que atraviesa dichas barreras. La altura de las barreras de Schottky determinada con el modelo es comparada con las alturas obtenidas a través del análisis de barrido por espectroscopía túnel (STS en la región del borde de grano.

  6. Detection of leptospires in clinically healthy piglets born from sows experimentally infected with Leptospira interrogans serovar Canicola Detecção de leptospiras em leitões clinicamente saudáveis nascidos de matrizes infectadas experimentalmente com Leptospira interrogans sorovar Canicola

    Directory of Open Access Journals (Sweden)

    Francisco Rafael M. Soto

    2006-12-01

    Full Text Available Leptospirosis is an important zoonosis that causes reproductive disorders in swine. The isolation of leptospires from aborted fetuses, stillbirths and weak piglets was obtained in several occasions, however, the bacteria was never isolated from apparently healthy piglets born from apparently healthy infected dams. Six sows of the Landrace breed with a known date of service and pregnancy confirmed by ultrasonography were infected intravenously with 5ml of Leptospira interrogans serovar Canicola inoculum between 76 and 90 days of gestation, and one week after farrowing, at least one piglet per sow was euthanized and samples of liver, kidneys, lungs, heart, spleen and gastric content were taken and examined by PCR. Reproductive disorders or any clinical sign of infection were not observed in the inoculated sows. The piglets born from these animals presented no clinical signs or macroscopic lesions that could be attributed to leptospirosis. All inoculated sows presented anti-leptospires antibodies by microscopic serum-agglutination test (MAT in the postinoculation serum samples and leptospires were not found in the urine as well as were not detected by PCR applied in this material, however, PCR accomplished in kidneys and liver from a euthanized sow presented positive results. Of the total of 12 euthanized piglets, 10 (83.3% presented positive results by PCR in at least one of the kidney, liver, heart, spleen, lung and gastric content samples. The present study reports the vertical transmission of the infection and the detection of leptospires in clinically healthy piglets born from experimentally infected sows, which is important of the epidemiological point of view as the maintenance of clinically healthy infected animals may allow the persistence of the bacteria in the herd, exposing other animals to the risk of infection.A Leptospirose é uma importante zoonose que causa transtornos reprodutivos em suínos. O isolamento de leptospiras de fetos

  7. Modelado de sistemas complejos mediante simulación basada en agentes y mediante dinámica de sistemas

    Directory of Open Access Journals (Sweden)

    LUIS R. IZQUIERDO

    2008-01-01

    Full Text Available Este trabajo compara dos técnicas de modelado de sistemas complejos: la simulación basada en agentes y la dinámica de sistemas. Esta comparación se lleva a cabo dentro del marco general del proceso de modelado científico. Los autores concluyen que la principal diferencia entre las dos metodologías se encuentra en el proceso de abstracción que cada una de ellas realiza para construir el modelo formal a partir del sistema complejo observado. Esta diferencia inicial se extiende a las restantes etapas del proceso de modelado científico. Finalmente, se indican los principales factores y las propiedades generales de un sistema complejo que hacen que una u otra técnica sea más relevante, aunque los autores destacan que, en la mayoría de los casos, modelizar un mismo sistema mediante las dos técnicas es la solución idónea.

  8. Changes in the boreal summer intraseasonal oscillation projected by the CNRM-CM5 model under the RCP 8.5 scenario

    Science.gov (United States)

    Li, Jianying; Mao, Jiangyu

    2016-12-01

    The 30-60-day boreal summer intraseasonal oscillation (BSISO) is the predominant intraseasonal variability in the Asian summer monsoon (ASM) region, representing the canonical northward and northwestward propagating convective components over South Asia (SA) and East Asia/western North Pacific (EA/WNP) sectors in conjunction with eastward propagating convective anomalies. The objective of this study is to assess possible changes of the 30-60-day BSISO in future global warming condition by comparing the twentieth century simulation with the twenty-first century projection produced by the CNRM-CM5 model under the representative concentration pathway 8.5 (RCP 8.5) scenario. In response to the increase of sea surface temperature in the tropical and subtropical Indian and Pacific Oceans, the saturation specific humidity in the planetary boundary layer (PBL) increases by about 16 %, providing more moisture and moist static energy for tropical convection. Thus, the BSISO will be intensified, with large-amplitude events prevailing in a broader range of the Indo-Pacific region. The convective signal will initiate over more westward parts of the Indian Ocean and decay over the more eastward tropical Pacific. As the strengthening of northward propagations over the SA and EA/WNP sectors is intimately related to equatorial enhanced convective anomalies, the enhanced convective anomalies are accompanied by stronger ascents on the top of the PBL, together with the wetter seasonal-mean PBL background, resulting in stronger northward propagations through moisture mechanisms. Moreover, due to the increased moisture-holding capacity of the low-level atmosphere, the phase speeds of SASM and EA/WNP northward propagation will decrease.

  9. 反应堆冷却剂泵转速测量关键部件辐照试验设计%Design of Irradiation Experiment for Key Component of RCP Speed Measuring

    Institute of Scientific and Technical Information of China (English)

    吴茜; 邱建文

    2016-01-01

    核电厂RCP系统是压水堆核电厂的关键设备之一。主泵转速监测设备的主要作用是监测反应堆冷却剂泵的转速。主泵转速频率电流转换器作为主泵转速监测设备的关键部件,属于K2类鉴定要求范畴,需要完成辐照试验。本文根据核电站现场辐照条件及用户使用寿命的需求,对辐照试验的试验方案和试验环境进行了设计。试验结论表明,试验方案可行有效,经验证,该设备在安装现场辐照剂量率不大于15mSv/h的环境下,能够满足5年正常工作的可靠性要求。%RCP system of nuclear power plants is one of the key equipment of PWR nuclear power plant. the main role of the RCP speed monitoring device is to monitor RCP speed. The key component of the RCP speed frequency current converter as a RCP speed monitoring equipment, belonging to the K2 class of identification category, need to complete the irradiation test. Based on the irradiation conditions on site and the user needs, test programs and test environments of irradiation tests were designed. Conclusion of the test showed that the test is feasible and effective, it proves the equipment in the installation site can meet the reliability requirements of 5 years work properly under the environment is not greater than the radiation dose rate 15mSv/h.

  10. Climático: evaluación mediante modelos

    Directory of Open Access Journals (Sweden)

    P. Ruiz-Benito

    2013-01-01

    Full Text Available Los bosques son ecosistemas fundamentales en la generación de servicios ecosistémicos y, por tanto, para el bienestar humano. El cambio global (incluyendo cambio climático y cambios en el uso del suelo puede, sin embargo, alterar la dinámica y el funcionamiento de los ecosistemas, afectando al futuro suministro de servicios ecosistémicos. La vulnerabilidad frente al cambio global depende de la exposición (magnitud del cambio, la sensibilidad (susceptibilidad al cambio, y la capacidad de adaptación (habilidad para ajustarse al cambio de las especies. En el presente trabajo presentamos diversas aproximaciones de modelización que permiten analizar los diferentes componentes de la vulnerabilidad, e incluimos ejemplos desarrollados para bosques de la península Ibérica. A pesar de estos avances, la evidencia empírica y teórica para integrar los impactos potenciales (i.e. incluyendo la exposición y la sensibilidad y la capacidad de adaptación de las especies, es escasa. Por ello, para una adecuada evaluación sería necesario me-jorar el conocimiento existente sobre la sensibilidad y capacidad de adaptación de las especies y su respuesta frente a cambios ambientales extremos (por ejemplo, mediante redes de seguimiento a largo plazo, integrando adecuadamente la información obtenida en modelos que incluyan procesos basados en diferentes niveles de organización biológica, desde procesos fisiológicos a modelos agregados de distribución de especies.

  11. Chirurgia della valvulopatia mitralica mediante Port Access. Esperienza di due centri.

    OpenAIRE

    Iorio, Francesco

    2014-01-01

    La chirurgia mininvasiva della mitrale mediante Port Access, risulta una delle maggiori innovazioni in cardiochirurgia in grado di migliorare gli outcome a breve medio e lungo termine se paragonata alla chirurgia convenzionale. In questo studio sono sono prese in esame le esperienze di due centri di eccellenza che hanno condotto tali interventi mediante approcci tecnici intraoperatori diversi per il clampaggio aortico: l'Endoclamp ed il clampaggio aortico transtoracico. Vengono altresì discus...

  12. Desarrollo de la lectura mediante estratégias integradoras

    Directory of Open Access Journals (Sweden)

    Solé, Maira

    2005-06-01

    Full Text Available La lectura y la escritura son procesos que cada día ameritan nuevos cambios y transformaciones. La propuesta de un Proyecto Pedagógico Integrador, (Fraca 2003 desarrollado con éxito en algunas instituciones venezolanas, se perfila como una alternativa significativa para el desarrollo de estos elementos. La idea o núcleo central es la integración de las diferentes asignaturas curriculares y lograr una globalización partiendo de sus objetivos y contenidos programáticos. El eje pedagógico integrador le permite al docente, evidenciar con mayor prontitud los resultados mediante actividades prácticas de lectura y escritura. Así mismo combina elementos claves del aprendizaje ausbeliano: información previa, información nueva y construcción de la información definitiva o integrada. La puesta en ejecución de las estrategias integradoras, en esta ocasión por maestros en formación (UNEG, a diferentes niños de escuelas del Estado Bolívar (Venezuela, certificando cómo la lectura y la escritura pueden tener un espacio ideal y significativo en la instrucción actual. Solo se necesita la intención, creatividad, dinamismo e ingenio. The reading and the writing plows processes that every day they require new changes and transformations. The proposal of an Integrative Pedagogic Project, (Fraca 2003 developed with success in some Venezuelan institutions; it is profiled like a significant alternative for the development of these elements. The idea or central nucleus is the integration of the different curricular subjects and to achieve a globalization leaving of its objectives and programmatic contents. The integrative pedagogic axis allows to the educational one, to evidence with more readiness the results by means of practical activities of reading and it notarizes. Likewise it combines key elements of the learning ausbeliano: previous information, new information and construction of the definitive or integrated information. The operation of

  13. de teorías mediante un estudio de caso

    Directory of Open Access Journals (Sweden)

    Verónica Alonso Jiménez

    2008-01-01

    Full Text Available La democracia representativa como forma de gobierno, implica que el poder se ejerce por personas, que elegidas por el pueblo, actúan en su nombre y representación. El modo de participación en la elección y la manera como éstas se convierten en cargos públicos, requiere del dise- ño de instituciones que sistematicen dicha participación. La república de tipo presidencial, es una modalidad del gobierno electivo y popular, cuyo titular es el jefe del ejecutivo, electo por el pueblo o sus representantes, en donde el ejercicio del poder es limitado y mantiene un régimen de responsabilidades políticas. El diseño institucional que le corresponde a esta forma de gobierno es la parcelación del poder pú- blico en tres: poder ejecutivo, poder legislativo y poder judicial. Las ventajas políticas de este esquema es que la división de poderes, neutraliza el riesgo de caer en el autoritarismo, al impedir que el poder se concentre. La división de poderes es un dispositivo de restricción de facultades de los órganos estatales, por lo que no existe superioridad jerárquica entre los poderes, al contrario, cada órgano tiene bien delimitadas sus funciones y atribuciones, las que están reguladas por un marco jurídico común llamado Constitución. Y la división de poderes contribuye a mantener el equilibrio entre estos, mediante el llamado sistema de “pesos y contrapesos”. El presente trabajo se centra en el estudio de la Cámara de Diputados, considerada como una de las parcelas en las que está divida la autoridad del Estado, cuyo objetivo es la validación de los modelos teóricos en la tipificación del Congreso y la comprensión de las variables internas y externas que influyen en el comportamiento legislativo.

  14. MASTITE E SÍNDROME DA QUEDA DO LEITE / INFECÇÃO POR Leptospira interrogans EM OVELHAS DA RAÇA SANTA INÊS NO DISTRITO FEDERAL

    Directory of Open Access Journals (Sweden)

    Adriana Helena Rosa

    2012-06-01

    Full Text Available The aim of the present work was to determine the relationship between clinical mastitis and Leptospira interrogans / Milk Drop Syndrome in Santa Inês ewes. One thousand sheep were examined on 12 farms in the Distrito Federal, Brazil, as to their clinical condition, in order to identify animals with clinical mastitis. The animals were divided into two groups: animals which presented clinical mastitis symptoms (G1 and animals which did not have clinical mastitis symptoms (G2. Blood samples were collected from all ewes of the two groups. The microscopic agglutination test was performed in order to identify the seropositive animals to Leptospira spp. Four (4.08% animals of the first group were seropositives to Leptospira spp., of which three were positives to Hardjoprajitno (Norma and Hardjoprajitno (OMS sorovars and one to both Australis and Autumnalis sorovars. In the second group, two (5.26% animals were seropositives to both Hardjoprajitno (Norma and Hardjoprajitno (OMS sorovars. A relationship between Leptospira spp. infection / milk drop syndrome and the presence of clinical mastitis in ewes (p > 0.05 was not observed.

  15. Changes of High Temperature Events During Rice Growth Period in MLRYR Under RCP Scenarios%RCP情景下长江中下游地区水稻生育期内高温事件的变化特征

    Institute of Scientific and Technical Information of China (English)

    冯灵芝; 熊伟; 居辉; 曹阳; 杨笛

    2015-01-01

    基于1981-2009年历史气象数据和全球气候模式HadGEM2-ES输出的未来2021-2050年RCP气候情景数据,采用日最高温度大于35℃的高温日数(HSD)、高温最长持续日数(MCD)和高温有效积温(HDD),分析过去和未来长江中下游地区水稻生育期高温事件频率、持续时间和强度的变化特征。结果表明:1981-2009年,长江中下游地区水稻生育期内温度升高,各高温指标均一致显著增加,Tmax(平均日最高气温)、HSD、MCD、HDD的气候倾向率分别为0.51℃·10a−1,3.9d·10a−1,0.6d·10a−1和8.2℃·d·10a−1,空间上表现为由北向南递增。除MCD外,Tmax、HSD和HDD均在2001-2002年发生由少到多的突变。2021-2050年两种RCP情景下,长江中下游大部分地区水稻生育期间日最高气温持续升高,高温日数增多,持续时间延长,高温强度增强。RCP2.6情景下,水稻生育期内Tmax、HSD、MCD、HDD较基准时段(1981-2009年)分别增加1.5℃、11.3d、5.6d和45.3℃·d,RCP8.5情景下分别增加1.7℃、15.4d、6.2d和61.1℃·d,且各高温事件在高值区的概率进一步加大。各指标的空间变化特征具有差异性,Tmax、HSD和MCD的增加幅度由东南向西北递增,湖南西部和江苏北部等基准期温度相对较低的地区增幅更大,而 HDD 的增幅以中部地区较大。湖北、安徽、湖南和江西中北部是未来高温事件频率、持续时间和强度均大幅增加的地区,防灾减灾工作严峻,需采取调整水稻播期,更替耐高温品种等措施减轻高温对水稻的危害。%Based on historical weather data from 1981 to 2009 and future climate data generated by HadGEM2-ES simulation with RCP2.6 and RCP8.5 emission scenarios from 2021 to 2050, frequency, duration and intensity of high temperature events during rice growth period in middle and lower reaches of Yangtze River (MLRYR) were analyzed by heat stress days (HSD

  16. 绵阳市1963~1997年钩端螺旋体病疫情及流行菌株变化分析%THE CHANGE OF LEPTOSPIROSIS AND THO STRAIN OF LEPTOSPIRA INTERROGANS IN MLANYANG IN 1963~1997

    Institute of Scientific and Technical Information of China (English)

    刘汉军; 刘昌弟; 周荣

    2000-01-01

    The article analyzed the change of Leptospirosis and strain of Leptospira interrogans in Mianyang in 1963-1997. The annual average incidencd rate is 4. 73 and the mortality rate is 0. 079 per one hundred thousand persons. In this area,the distribution of Leptospirosis agreed on others internal reports in sex ,age,profession and seasonlity. 5 outbreaks have been taken place in 35 years. Four of which were major Icterohaemorrhagiae serogroup Icterohaemorrhagiae sertoype and one was Hebdomadis Serogroup Hebdomadis Serotype. Leptospira 7 Serogroup and 10serotype have been found in 49 Leptospira interrogans which have been cultivated from blood of patients. It supports that rodent is the principal infections source of Leptospirosis that 174 Leptospira interrogans have been isolated from rodent.%本文报告了四川绵阳市35年钩端螺旋体病(下称钩体)疫情及流行菌株情况,年平均发病率4.73/10万,年平均死亡率0.079/10万,钩体病的性别、年龄、职业及季节分布与国内报道相一致。35年中发生5次局部暴发流行,4次以黄疽出血型为主,一次以七日热型为主。从病人血分离到49株钩体菌,分属七群10个血清型,从动物体内分离到菌株174株,证实啮齿类动物为主要传染源。

  17. Analysis of Leptospira interrogans ompA gene and immunological identification of its recombinant expression product%问号钩端螺旋体ompA基因分析及其重组表达产物的免疫学鉴定

    Institute of Scientific and Technical Information of China (English)

    丁威; 董海艳; 薛峰; 严杰; 楼永良

    2009-01-01

    Objective To investigate the distribution of ompA gnne in 15 Chinese reference standard strains belonging to 15 serogroups of Leptospira interrogate, and to express recombinant OmpA ( rOmpA ) and to identify immunogenicity and immunoprotection of rOmpA. Methods Genomic DNAs from different leptospiral strains were extracted by phenol-chloroform method. Entire ompA gene fragments from the strains were amplified by PCR and then sequenced after T-A cloning. A prokaryotic expression system of ompA gene from L. interrogans strain 56601 was constructed, and the expression and yield of rOmpA were determined by SOS-PAGE plus Bio-Rad Agarose Image Analyser. Rabbits were immunized with rOmpA for obtaining antiserum, and immunodiffusion test was used to measure the antiserum's titer. Western blot assay was performed to determine the immunoreaetivity of rOmpA with the antiserum against rOmpA and antiserum against whole cell of L. interrogans strain 56601, while mi-croscopic agglutination test (MAT) was applied to detect the cross agglutination to the 15 L. interrogans strains. A leptospire adhering cell model and a leptospire infecting guinea pigs model were used to determine the adhesion-bloc-king effect of rOmpA antiserum and immunoprotection of rOmpA. Results All the 15 L. interrogans strains, but not L. biflexa strain Patoe Ⅰ , had sequence conserved ompA genes. The yield of rOmpA was approximate 20% of the total bacterial proteins, rOmpA could induce rabbits to produce antibody and immunodiffusion titer of the anti-serum was 1:4. Both antisera against rOmpA and against whole cell of L. interrogans strain 56601 were able to pro-duce positive Western blot signs to rOmpA, and the former offered 1 : 20-1 : 320 MAT titers to the 15 L. interrogans strains. 1: 10-1:160 dilutions of rOmpA antiserum could efficiently block L. interrogans strain 56601 adhering to J774A. 1 cells, and 100 μg and 200 μg rOmpA displayed 50.0% and 75.0% immunoprotective rates in the infee-ted guinea

  18. Control de un péndulo invertido mediante nodos CAN

    OpenAIRE

    2004-01-01

    En líneas generales, el propósito de este proyecto, es realizar el control de la maqueta de un péndulo invertido del departamento de ESAII. Esta maqueta está compuesta por una estructura de aluminio, sobre la cual un carro se desliza sobre una superficie plana enganchado a un motor mediante una correa. En este motor se encuentra un encoder óptico, en el extremo de su eje mediante un acoplamiento. En el carro se encuentra el péndulo, el cual es doble, es decir el péndulo cuelga por delante...

  19. Identificación en lazo cerrado mediante algoritmos genéticos

    OpenAIRE

    Alfredo Gómez Infante; Abelardo del Pozo Quintero; Alberto Aguado Behar

    2010-01-01

    La identificación  en lazo cerrado de sistemas continuos es un problema de optimización no lineal que resulta de difícil solución mediante métodos convencionales.  En este trabajo se propone el uso de algoritmos genéticos (AG) para esta tarea y se muestra mediante simulaciones que los modelos obtenidos pueden reproducir fielmente el comportamiento de la planta, aun en el caso de sistemas inestables o que presentan algún tipo de no-linealidad. Estos modelos pueden utilizarse para el ajuste ...

  20. Tratamiento de tumores óseos benignos mediante legrado y relleno con metilmetacrilato

    OpenAIRE

    Orduña Serra, Modesto; Fernández Sabaté, Alfons; Amorós, P.

    1989-01-01

    Se expone la experiencia en ocho lesiones óseas tumorales y paratumorales benignas tratadas mediante legrado de la cavidad y posterior relleno mediante metilmetacrilato con contraste baritado. El seguimiento de los casos entre año y medio y once años permite que nos afirmemos en la bondad de los resultados en cuanto a función articular resistencia de la extremidad y control del tumor. Comentamos las estadísticas publicadas en las que destacan los casos de tumor de células gigantes (66 por 100...

  1. Tratamiento del choque femoroacetabular mediante miniabordaje anterior. Resultados a corto plazo

    OpenAIRE

    Diranzo García, J.; Estrems Díaz, V.; Hernández Ferrando, L.; Castillo Ruiperez, L.; Brú Pomer, Antonio

    2015-01-01

    El tratamiento quirúrgico del choque femoroacetabular (CFA) es un práctica clínica cada vez más frecuente en nuestra especialidad. Objetivo. Analizar los resultados clínicos y radiológicos de una primera serie de pacientes diagnosticados de CFA intervenidos mediante miniabordaje anterior. Material y métodos. Estudio prospectivo de 30 pacientes con una edad media de 36,2 años y un seguimiento mínimo de 12 meses. La valoración clínica se ha realizado mediante las escalas SF-36, WOMAC y NAHS. Ev...

  2. Modulación del crecimiento vertebral mediante electrocoagulación hemicircunferencial vertebral asistida

    OpenAIRE

    2009-01-01

    Nuestro trabajo está basado en la posibilidad de controlar el desarrollo asimétrico de los cartílagos de crecimiento vertebral, mediante la realización de una fisiodesis hemivertebral, con electrocoagulación, videoasistida por toracoscópica. Se realizará en cinco niveles torácicos, con un abordaje anterior mínimamente invasivo. Por lo tanto, planteamos como hipótesis de trabajo que La destrucción de las fisis de crecimiento vertebral mediante electrocoagulación, videoasistida por vía toracosc...

  3. Recuperación de boro mediante extracción y procesos de membrana

    OpenAIRE

    Gallego Latorre, Maria Irene

    2008-01-01

    En este proyecto se ha estudiado la posibilidad de extraer boro en forma de ácido bórico mediante extractantes neutros. En primer lugar se han realizado los experimentos de extracción líquido-líquido y después se han realizado pruebas de extracción mediante membranas planas soportadas. Las pruebas de extracción líquido-líquido han permitido estudiar el comportamiento del boro ante diferentes extractantes neutros (2,2,4-Trimethyl-1,3-pentanodiol , 2- Butyl-2-ethyl-1,3-p...

  4. Control PID de un secador mediante autómatas programables conectados por ethernet

    OpenAIRE

    Carrillo Valencia, Víctor

    2012-01-01

    Este proyecto final de carrera se centra en el control PID de temperatura de un secador convencional mediante autómatas programables conectados por Ethernet. Se busca con ello la configuración de un red de comunicaciones entre dos PLC’s industriales (Programmable Logic Controller en sus siglas en inglés) y el control de temperatura mediante un controlador PID. Los controladores lógicos programables o PLC’s son dispositivos electrónicos muy usados en automatización industrial...

  5. Control PID de un secador mediante autómatas programables conectados por ethernet

    OpenAIRE

    Carrillo Valencia, Víctor

    2012-01-01

    Este proyecto final de carrera se centra en el control PID de temperatura de un secador convencional mediante autómatas programables conectados por Ethernet. Se busca con ello la configuración de un red de comunicaciones entre dos PLC’s industriales (Programmable Logic Controller en sus siglas en inglés) y el control de temperatura mediante un controlador PID. Los controladores lógicos programables o PLC’s son dispositivos electrónicos muy usados en automatización industrial...

  6. Daño muscular inducido mediante ejercicio y sus efectos en el rendimiento

    OpenAIRE

    Valenzuela Tallón, Pedro Luis

    2014-01-01

    El presente trabajo pretende introducir un tema de gran controversia: el daño muscular inducido mediante ejercicio (DMIE). Se ha realizado una revisión bibliográfica de la literatura científica referente al DMIE para exponer la teoría más actual en cuanto al mecanismo que lo provoca y los cambios biológicos y funcionales producidos tras éste. El DMIE comienza como un proceso mecánico mediante el cual se dañan las estructuras de las fibras musculares, lo cual da lugar a un proceso inflamatorio...

  7. Tratamiento de aguas residuales industriales mediante electrocoagulación y coagulación convencional

    OpenAIRE

    Martínez Navarro, Fabiola

    2011-01-01

    Existen numerosos tipos de aguas y aguas residuales (las aguas de abastecimiento, efluentes industriales consistentes en suspensiones coloidales, aguas residuales contaminadas con macromoléculas orgánicas disueltas o las emulsiones de aceite en agua (o/w) susceptibles de ser tratadas mediante coagulación, una operación dirigida a la consecución de la desestabilización de los contaminantes mediante su interacción con un reactivo (generalmente, sales de fe(III) y de al(III)). Esta operación pue...

  8. Serología positiva a Leptospira interrogans, serovar cynopteri en caninos de la Ciudad de Buenos Aires, Argentina Cynopteri as a new serovar of Leptospira interrogansin canines of Buenos Aires, Argentina

    Directory of Open Access Journals (Sweden)

    M.S Tealdo

    2007-12-01

    Full Text Available La Leptospirosis es una enfermedad infectocontagiosa, producida por una bacteria del género Leptospira, que ataca indistintamente al hombre y a animales domésticos y silvestres. El agente puede vivir y reproducirse en aguas de ríos, arroyos, lagos, aguas estancadas, etc. Es una patología polimorfa. Los caninos pueden actuar como fuente de infección para el hombre debido al estrecho contacto que se desarrolla entre ambos. Tradicionalmente al género Leptospira se lo considera integrado por dos especies: L. interrogans (formas patógenas y L. biflexa (abarca formas saprófitas. Estas especies a su vez, se dividen en serogrupos y serovares. Entre junio y septiembre de 2003 se recolectaron 110 muestras de suero de caninos que concurrieron al Instituto de Zoonosis Luis Pasteur (IZLP. Los sueros fueron procesados en los laboratorios de Diagnóstico de Leptospirosis del IZLP y del Instituto de Patobiología del INTA, Castelar (Provincia de Buenos Aires, con la técnica de microaglutinación en tubo (Técnica de Martín y Petit. Las muestras se enfrentaron a cepas de 23 serovares, resultando en una seroprevalencia del 33%. Un dato inédito fue el alto índice (59% de seropositividad (en su mayoría reaccionante a títulos 1/100 al serovar cynopteri. De los animales positivos (51% machos y 49% hembras, 30% provenían de barrios de zonas marginales y villas de emergencia y un 70% del resto de la ciudad. Un número de factores pudieron haber cambiado en los últimos años debido a la crisis económica (presencia de cartoneros, el uso de caballos para tracción en el ámbito de la ciudad y la cría clandestina de cerdos. Dado que estos valores muestran un incremento de la seroprevalencia respecto de los valores históricos para la ciudad y la detección del serovar cynopteri en un alto porcentaje de muestras, se recomienda considerar la inclusión de más serovares en los testeos serológicos en caninos.Leptospirosis is an infectious illness caused by

  9. Comparison between generalized linear modelling and additive Bayesian network; identification of factors associated with the incidence of antibodies against Leptospira interrogans sv Pomona in meat workers in New Zealand.

    Science.gov (United States)

    Pittavino, M; Dreyfus, A; Heuer, C; Benschop, J; Wilson, P; Collins-Emerson, J; Torgerson, P R; Furrer, R

    2017-09-01

    Additive Bayesian Network (ABN) is a graphical model which extends Generalized Linear Modelling (GLM) to multiple dependent variables. The present study compares results from GLM with those from ABN analysis used to identify factors associated with Leptospira interrogans sv Pomona (Pomona) infection by exploring the advantages and disadvantages of these two methodologies, to corroborate inferences informing health and safety measures at abattoirs in New Zealand (NZ). In a cohort study in four sheep slaughtering abattoirs in NZ, sera were collected twice a year from 384 meat workers and tested by Microscopic Agglutination with a 91% sensitivity and 94% specificity for Pomona. The study primarily addressed the effect of work position, personal protective equipment (PPE) and non-work related exposures such as hunting on a new infection with Pomona. Significantly associated with Pomona were "Work position" and two "Abattoirs" (GLM), and "Work position" (ABN). The odds of Pomona infection (OR, [95% CI]) was highest at stunning and hide removal (ABN 41.0, [6.9-1044.2]; GLM 57.0, [6.9-473.3]), followed by removal of intestines, bladder, and kidneys (ABN 30.7, [4.9-788.4]; GLM 33.8, [4.2-271.1]). Wearing a facemask, glasses or gloves (PPE) did not result as a protective factor in GLM or ABN. The odds of Pomona infection was highest at stunning and hide removal. PPE did not show any indication of being protective in GLM or ABN. In ABN all relationships between variables are modelled; hence it has an advantage over GLM due to its capacity to capture the natural complexity of data more effectively. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

  10. Influência da via de inoculação sobre o estabelecimento e a evolução da leptospirose em hamsters (Mesocricetus auratus experimentalmente infectados com Leptospira interrogans sorovar pomona

    Directory of Open Access Journals (Sweden)

    Nicodemos Alves de Macedo

    2004-06-01

    Full Text Available Foi investigada a influência da via de inoculação sobre o estabelecimento e a evolução da leptospirose em hamsters (Mesocricetus auratus experimentalmente infectados com Leptospira interrogans sorovar pomona. As vias de inoculação ensaiadas foram: intraperitoneal, subcutânea, oral, conjuntival e escarificação cutânea. O inóculo infeccioso foi constituído por uma cultura em meio de Fletcher, com 20 a 30 leptospiras por campo microscópio no aumento de 200 vezes. Os animais controle foram inoculados apenas com meio de Fletcher. Foram colhidas amostras de soro sanguíneo e fragmentos de rins na fase agônica da doença ou no 21º dia pós-infecção, quando todos os animais foram sacrificados. Para a pesquisa de leptospiras, foi feito o exame direto com microscopia óptica em campo escuro e cultivo em meio de Fletcher, pela técnica das diluições seriadas. A detecção de aglutininas anti-leptospiras foi realizada pela técnica de soroaglutinação microscópica. A instalação e evolução da leptospirose foram afetadas pela via de inoculação. A via oral foi a menos efetiva em estabelecer a infecção. Não foi observada associação estatística entre a freqüência de portadores e a via de inoculação.

  11. Método de eliminación de trihalometanos y/o contaminantes emergentes mediante plasma

    OpenAIRE

    Molina, Ricardo; Erra Serrabasa, Pilar; Bertrán, Enric; Jover Comas, Eric; Reyes Contreras, Carolina

    2009-01-01

    Método de eliminación de trihalometanos y/o contaminantes emergentes mediante plasma. Se describe un método de eliminación de trihalometanos y contaminantes refractarios en medios acuosos mediante la aplicación directa de plasma para conseguir la degradación de los compuestos contaminantes presentes en el agua.

  12. Procesamiento de muestras fecales en el estudio de Cryptosporidium sp. mediante PCR

    Directory of Open Access Journals (Sweden)

    Gregorio Pérez-Cordón

    2013-05-01

    Full Text Available El presente trabajo describe un protocolo aplicado a muestras de heces de ganado bovino para el aislamiento y purificación de ooquistes de Cryptosporidium sp. y el eventual uso de los mismos para estudios genéticos mediante PCR.

  13. Derrame pleural tuberculoso: diagnóstico mediante videotoracoscopia Derrame pleural tuberculoso: diagnóstico mediante videotoracoscopia Tuberculous pleural effusion: diagnosis by videothoracoscopy

    OpenAIRE

    Edelberto Fuentes Valdés; Miguel A Martín González

    2006-01-01

    El diagnóstico de la tuberculosis pleural es difícil por la presentación inespecífica y la pobre eficiencia de los métodos diagnósticos tradicionales cuando de esta se trata. Se realizó un estudio retrospectivo de 6 pacientes a quienes se realizó el diagnóstico de tuberculosis pleural mediante videotoracoscopia, ante el fracaso de los métodos tradicionales. El objetivo fue conocer el valor de la videotoracoscopia en el diagnóstico de la tuberculosis pleural. Cinco de los 6 pacientes eran del ...

  14. Purification and Initial Studies of the Water-Soluble Polysaccharide RCP From the Roots of Rubus crataegiflolius Bge%托盘根水溶性多糖的分离纯化及初步研究

    Institute of Scientific and Technical Information of China (English)

    张旭; 房金波; 梁忠岩

    2005-01-01

    托盘根经热水煮提、醇析、脱淀粉、冻融离心、蛋白酶解、Sevagc法脱蛋白、超滤、凝胶柱层析方法纯化得水溶性多糖RCP,经比旋光度测定、高效液相色谱(HPLC)分析证明RCP为均一组分,分子量7000左右;气相色谱(GC)分析其单糖组成主要为葡萄糖和迹量的鼠李糖.说明RCP是一种中性杂多糖.

  15. The Analysis of the RCP Financing Mode in West Water Conservancy Construction Project%西部公益型水利建设项目应用RCP融资模式的探析

    Institute of Scientific and Technical Information of China (English)

    郭峰; 舒灵智; 廖博

    2009-01-01

    为了解决西部地区公益型水利建设项目融资困难的问题,引入RCP(资源-补偿-项目)融资模式,分析了我国现行水利项目融资基本模式和存在问题,对RCP融资模式进行了概述,并运用SWOT分析法论证了西部公益性水利建设项目中应用RCP融资模式的可行性.这种融资模式能够吸引更多民间资本参与水利项目的建设.提高社会资金的使用效率,为解决水利建设中的融资难题提供了途径和可能.

  16. RCP01: a Monte Carlo program for solving neutron and photon transport problems in three-dimensional geometry with detailed energy description (LWBR development program). [For CDC-6600 and -7600, in FORTRAN

    Energy Technology Data Exchange (ETDEWEB)

    Candelore, N R; Gast, R C; Ondis, II, L A

    1978-08-01

    The RCP01 Monte Carlo program for the CDC-7600 and CDC-6600 performs fixed source or eigenfunction neutron reaction rate calculations, or photon reaction rate calculations, for complex geometries. The photon calculations may be linked to the neutron reaction rate calculations. For neutron calculations, the full energy range is treated as required for neutron birth by the fission process and the subsequent neutron slowing down and thermalization, i.e., 10 MeV to 0 eV; for photon calculations the same energy range is treated. The detailed cross sections required for the neutron or photon collision processes are provided by RCPL1. This report provides details of the various types of neutron and photon starts and collisions, the common geometry tracking, and the input required. 37 figures, 1 table.

  17. Identification and characterization of intermediates in the formation of the cyclobutadiene linked dimeric cobalt sandwich compound [(5-RCp)Co(4-C4Ph3)]2 [R = H, CH3C(O)O

    Indian Academy of Sciences (India)

    Nem Singh; Anil J Elias

    2011-11-01

    The synthesis of novel cyclobutadiene linked dimers of [5-RCp]Co(4-C4Ph4) was reported recently from a three-component reaction of 1,4-diphenylbutadiyne, diphenylacetylene and [5-RCp]Co(PPh3)2 [R = H or MeOC(O)]. The chiral bis oxazoline derivative of this dimer was found to form cobalt oxazolinyl palladacycles which were excellent chiral catalysts for the Overman-Claisen rearrangement of trichloroacetimidates, similar to the well-known COP catalysts. In this paper, we report reactions carried out for identifying the intermediates in the formation of the dimeric sandwich compound {[5-MeOC(O)Cp]Co(4-C4Ph3)}2 (1). On varying the stoichiometric ratio of the reactants of the three component reaction, two new cobalt sandwich compounds [5-MeOC(O)Cp]Co[4-C4Ph3(C≡CPh)] (2) and [5-MeOC(O)Cp]Co[4-C4-Ph2(C≡CPh)2] (3) were found to form as viscous oily liquids. The identity of 2 was ascertained by oxidizing its alkyne moiety to yield crystalline [5-MeOC(O)Cp]Co{4-C4Ph3[C(O)-C(O)Ph]} (4) which was structurally characterized. Further, the role of phenylethynyl derived monomeric sandwich compound 2 as a precursor to the dimeric compound 1 was established by reacting 2 with diphenylacetylene and CpCo(COD) which yielded the first example of an unsymmetrical cyclobutadiene linked dimeric cobalt sandwich compound {5-[MeOC(O)Cp]Co(4-C4Ph3)}[(5-Cp)Co(4-C4Ph3)] (5) which was also structurally characterized.

  18. ANATOMÍA DE LA PRIMERA PREMOLAR MANDIBULAR OBSERVADA MEDIANTE TOMOGRAFÍA CONE BEAM. ESTUDIO IN VITRO

    OpenAIRE

    Falla Coronel, Maricarmen; Universidad Señor de Sipán; Ibáñez Sevilla, Carmen Teresa; Universidad Nacional de Trujillo

    2016-01-01

    Objetivo: Determinar la anatomía de la primera premolar mandibular observada mediante tomografía Cone Beam mediante el estudio in vitro Material y métodos: Investigación cuantitativa, diseño transversal, se empleó 62 primeras premolares mandibulares extraídos por indicación de ortodoncia, enfermedad periodontal y caries dental. Se determinó la morfología externa de la raíz del primer premolar mandibular, el número de conductos de la primera premolar mandibular observadas mediante la tomografí...

  19. Programación por metas Energía alternativa mediante biomasa.

    Directory of Open Access Journals (Sweden)

    Guerrero Casas, Flor María

    2003-01-01

    Full Text Available En este trabajo se presenta un modelo multicriterio de localización de centrales de generación de energía eléctrica mediante biomasa. Los objetivos considerados son: (1 minimizar el coste total de la operación, (2 maximizar la producción de electricidad obtenida, (3 maximizar la distancia entre plantas, (4 maximizar la aceptación social y (5 establecer las plantas o ampliaciones en aquellos lugares donde exista una mayor predisposición por parte de las administraciones locales. Finalmente, se concluye con una aplicación práctica mediante programación por metas ponderadas para la región andaluza, considerando los residuos procedentes del olivar como fuente de energía.

  20. Materiales conductores mediante funcionalización de polímeros con nanomateriales conductores

    OpenAIRE

    Gutierrez, Manuel; Llobera, Andreu; Fernández Sánchez, César; Jiménez Jorquera, Cecilia; Mendoza Gómez, Ernest

    2010-01-01

    La presente invención se refiere un material conductor que comprende un polímero funcionalizado y a un nanomaterial conductor unidos mediante enlace covalente. La invención también se refiere a un procedimiento de obtención del material conductor sin modificar las propiedades ópticas del polímero y a su uso para la fabricación de sensores o electrodos.

  1. Materiales conductores mediante funcionalización de polímeros con nanomateriales conductores

    OpenAIRE

    Gutierrez, Manuel; Llobera, Andreu; Fernández Sánchez, César; Jiménez Jorquera, Cecilia; Mendoza Gómez, Ernest

    2010-01-01

    La presente invención se refiere un material conductor que comprende un polímero funcionalizado y a un nanomaterial conductor unidos mediante enlace covalente. La invención también se refiere a un procedimiento de obtención del material conductor sin modificar las propiedades ópticas del polímero y a su uso para la fabricación de sensores o electrodos.

  2. Miglioramento del profilo biofarmaceutico dell'albendazolo mediante co-cristallizzazione

    OpenAIRE

    Pranzo, Marco Beniamino

    2010-01-01

    Lo scopo del presente lavoro di tesi è stato quello di studiare il possibile miglioramento del profilo biofarmaceutico dell’ albendazolo attraverso una modificazione delle proprietà chimico-fisiche del farmaco stesso. Tale obiettivo è stato perseguito mediante: - ricristallizzazione del farmaco in solventi differenti al fine di isolare eventuali polimorfi; - studio delle interazioni allo stato solido tra il farmaco e vari CCFs al fine di ottenere un co-cristallo con caratteristiche ...

  3. A study of simulation and prediction of extratropical cyclones over the Northern Hemisphere partⅡ:Future changes under RCP4.5 projected by the 6 CMIP5 coupled models%北半球温带气旋的模拟和预估研究Ⅱ∶6个 CMIP5耦合模式预估的未来 RCP4.5情景下的变化

    Institute of Scientific and Technical Information of China (English)

    张颖娴; 丁一汇

    2014-01-01

    The simulation results of the 6 climate coupled models of CMIP5 under the secondary emission scenarion RCP4.5 (Representative Concentration Pathway 4.5)are used to estimate the changes of the extratropical cyclone (EC)numbers and EC intensity,as well as the storm track and its strength in the future.The results indicate that:(1)although each model dif¯ fers in the simulations of the regional cyclogenesis during 2053—2100 under RCP4.5 relative to the second half of the 20th cen¯ tury,it is similarly found that ECs would decrease integrally in the Northern Hemisphere,especially in the lower latitudes.(2) The central pressure of ECs over the Northern Hemisphere would depress and the vorticity intensity would weaken in the future reproduced by models consistently.The simulated Atlantic storm track would move polewards sequentially but with weakened strength as are shown by most of the models in the future;the simulated Pacific storm track would also migrate polewards by more than half the models,but with intensity changes differing in the different seasons.And,(3)a poleward movement of the future baroclinic zone in the middle¯upper troposphere is detected in all the 6 models,and this change in the Southern Hemi¯ sphere would be more significant.The changes of the baroclinic zone reflect the similar changes of storm track to some extent, which supports for the conclusion of possible poleward shift of the two storm tracks.That could be a significant reduction in EC activities over the Northern Hemisphere under RCP4.5,while the poleward movements of baroclinic zone and storm path would cause more remarkable reduction of EC activities over the lower latitudes in the future.%利用 CMIP5(Coupled Model Intercomparison Project Phase 5)的6个气候耦合模式中等排放情景———RCP4•5(典型浓度路径4•5)下的模拟结果对北半球温带气旋数目、风暴路径和强度的未来变化进行了研究分析。结果表明:(1)相对于20

  4. Determinación de la estructura poblacional de litopenaeus vannamei mediante issrs a lo largo de la costa ecuatoriana

    OpenAIRE

    Fresneda Rodríguez, Adriana

    2003-01-01

    Determinación de la estructura poblacional de Litopenaeus vannamei mediante ISSRs a lo largo de la costa ecuatoriana Muestras de Litopenaeus vannamei fueron colectadas en siete localidades a lo largo de la costa ecuatoriana desde Esmeraldas hasta Machala.

  5. SIMULACIÓN DEL COLAPSO EN TÚNELES DOVELADOS MEDIANTE LA FORMACIÓN DE ARTICULACIONES EN VIGAS

    OpenAIRE

    Enrique Tenorio Montero; Gelacio Juárez Luna

    2013-01-01

    Se estudia el mecanismo de colapso en túneles construidos con dovelas mediante el método los de elementos finitos. Los túneles se discretizaron mediante elementos finitos viga, resortes longitudinales y rotacionales, estos últimos tienen la capacidad de simular el desarrollo de articulaciones y el comportamiento de las juntas longitudinales, considerando un comportamiento constitutivo momento-rotación de las dovelas. En los apoyos se colocaron resortes axiales elásticos que representan la rig...

  6. Resposta humoral, recuperação bacteriana e lesões histológicas em camundongos geneticamente selecionados para bons e maus produtores de anticorpos e Balb/c, frente à infecção por Leptospira interrogans sorovar icterohaemorrhagiae Humoral immune response, bacterial recovery and time lesions in mice geneticaly selected for high and low antibody production and in outbreed Balb/c mice face to Leptospira interrogans sorovar icterohaemorrhagiae

    Directory of Open Access Journals (Sweden)

    Márcia Marinho

    2003-01-01

    Full Text Available O presente trabalho teve por finalidade associar a cinética da resposta humoral à recuperação de leptospiras viáveis e à intensidade das lesões teciduais em camundongos geneticamente selecionados para bons (High e maus (Low produtores de anticorpos (seleção IV-A, além de camundongos outbreed, Balb/c, inoculados com amostra patogênica de Leptospira interrogans sorovar icterohaemorrhagiae. As linhagens High e Low (seleção IV-A apresentam modificações em alguns compartimentos da resposta imune, principalmente em relação à atividade macrofágica, representando fenótipos extremos encontrados em populações naturais heterogêneas. Os camundongos foram sacrificados em oito momentos após a infecção. A análise dos resultados revelou que a partir do 7º dia após a infecção, os camundongos da linhagem High apresentaram elevação nos títulos de anticorpos estatisticamente significantes quando comparados aos camundongos da linhagem Low, mantendo assim o efeito multiespecífico. Os camundongos Balb/c apresentaram resultados intermediários entre as duas linhagens. A produção de anticorpos colaborou como fator limitante à infecção, pois quando obteve-se maior recuperação de leptospiras, na fase inicial da infecção, os títulos de anticorpos encontravam-se em elevação. As lesões observadas nos órgãos de camundongos infectados consistiram basicamente nos mesmos processos inflamatórios e degenerativos, que não se alteraram, variando apenas o grau de comprometimento tecidual, de acordo com a linhagem. A linhagem high apresentou lesões mais extensas que as apresentadas pelas linhagens low e Balb/c, sendo que nesta última as lesões foram moderadas. De forma geral a linhagem High e Balb/c apresentaram um perfil de resposta Th2, com o maior índice de produção de anticorpos e gravidade das lesões, enquanto a linhagem Low apresentou um perfil de resposta Th1.The aim of the present work was to evaluate the association

  7. Near Future (2016-40) Summer Precipitation Changes over China as Projected by a Regional Climate Model (RCM) under the RCP8.5 Emissions Scenario: Comparison between RCM Downscaling and the Driving GCM

    Institute of Scientific and Technical Information of China (English)

    ZOU Liwei; ZHOU Tianjun

    2013-01-01

    Multi-decadal high resolution simulations over the CORDEX East Asia domain were performed with the regional climate model RegCM3 nested within the Flexible Global Ocean-Atmosphere-Land System model,Grid-point Version 2 (FGOALS-g2).Two sets of simulations were conducted at the resolution of 50 km,one for present day (1980-2005) and another for near-future climate (2015-40) under the Representative Concentration Pathways 8.5 (RCP8.5) scenario.Results show that RegCM3 adds value with respect to FGOALS-g2 in simulating the spatial patterns of summer total and extreme precipitation over China for present day climate.The major deficiency is that RegCM3 underestimates both total and extreme precipitation over the Yangtze River valley.The potential changes in total and extreme precipitation over China in summer under the RCP8.5 scenario were analyzed.Both RegCM3 and FGOALS-g2 results show that total and extreme precipitation tend to increase over northeastern China and the Tibetan Plateau,but tend to decrease over southeastern China.In both RegCM3 and FGOALS-g2,the change in extreme precipitation is weaker than that for total precipitation.RegCM3 projects much stronger amplitude of total and extreme precipitation changes and provides more regional-scale features than FGOALS-g2.A large uncertainty is found over the Yangtze River valley,where RegCM3 and FGOALS-g2 project opposite signs in terms of precipitation changes.The projected change of vertically integrated water vapor flux convergence generally follows the changes in total and extreme precipitation in both RegCM3 and FGOALS-g2,while the amplitude of change is stronger in RegCM3.Results suggest that the spatial pattern of projected precipitation changes may be more affected by the changes in water vapor flux convergence,rather than moisture content itself.

  8. Functional analysis of Leptospira interrogans sphingomyelinase hemolysin genes and their transcriptional level alterations in the infected host cells%问号钩端螺旋体鞘磷脂酶类溶血素基因功能分析及其感染细胞后转录水平变化

    Institute of Scientific and Technical Information of China (English)

    赵金方; 林旭嫒; 严杰

    2009-01-01

    Objective To determine the hemolytic activity of products of sphingomyelinase hemolysin encoding genes of Leptospira interrogaas, and the transcriptional level alterations in the infected host cells. Methods By using genomic DNAs of pathogenic L. interrogans serovar serogroup Icterohaemorrhagiae serovar Lai strain Lai and serogroup Pomona serovar Pomona strain Luo, and non-pathogenic L. biflexa serogroup Sama-ranga serovar Patoc strain Patoc Ⅰ as templates, PCRs were performed to amplify entire sph1-sph4 genes. The amplified products were sequenced after T-A cloning. Prokaryotic expression systems of sph1-sph4 genes were re-spectively constructed, and the expressions of target recombinant proteins rSph1-rSph4 were examined by SDS-PAGE. Ni-NTA affinity chromatographic column was used to extract the expressed rSph1-rSph4. Hemolytic ac-tivities of rSph1-rSph4 on sheep blood agar plate were identified. Transcription alterations of sphl-sph4 genes in L. interrogans strain Lai after infected J774A. 1 cells were measured by real-time fluorescence quantitative RT-PCR. Results From genomic DNAs of both L. interrogans strain Lai and Luo, but not from that of L. biflexa strain Patoc Ⅰ , the target fragments of sph1-sph4 genes could be amplified. All the cloned sph1-sph4 genes had 100% nucleotide sequence identities compared to the corresponding reported sequences. The constructed pro-karyotic expression systems were able to efficiently express the target recombinant proteins rSph1-rSph4, respec-tively. All the rSph1-rSph4 had hemolytic activities, and among the four products rSph2 displayed the strongest hemolytic activity. After L. interrogaas strain Lai infecting J774A. 1 cells, the transcriptional levels of sph1-sph4 genes were remarkably up-regulated, especially for mRNA levels of sph2 and sph4 genes. Conclusion sph1- sph4 genes exist only in pathogenic L. interrogans species, and their products have hemolytic activity. The up-regulation of sph1-sph4 gene

  9. Resolución de equivalencias financieras mediante ecuaciones con coeficientes borrosos

    Directory of Open Access Journals (Sweden)

    Maria Silvia Moriñigo

    2007-01-01

    Full Text Available A menudo sólo se conocen estimaciones de las variables financieras. Es usual que con objeto de utilizar modelos clásicos, apreciaciones como “una tasa de entre el 5% y el 7%”, se conviertan en cantidades exactas, como puede ser el promedio entre los valores extremos. En este trabajo se propone un enfoque más flexible que permite captar la incertidumbre mediante la utilización de algunos elementos de la teoría de los conjuntos borrosos. La imprecisión presente en el capital, interés y/o cantidad de períodos se modela mediante números borrosos triangulares. Al apelar a los enfoques clásicos para evaluar expresiones algebraicas con coeficientes borrosos que hacen uso del principio de extensión y aritmética de -cortes, se definen las extensiones fuzzy de las relaciones financieras elementales. Se obtienen las versiones fuzzy del valor actual y del valor final de un capital borroso y el VAN mediante la resolución de ecuaciones con coeficientes borrosos por el método de α - cortes. Estos desarrollos se aplican a distintos casos de estudio. Por último, se muestra que no siempre es posible hallar un análogo borroso de la TIR utilizando los métodos clásicos de resolución de ecuaciones con coeficientes borrosos. Se calcula valiéndose de un nuevo concepto de solución

  10. Gluteoplastia tridimensional mediante distribución volumétrica precisa

    Directory of Open Access Journals (Sweden)

    R. Alfonso Vallarta-Rodríguez

    Full Text Available Introducción y objetivo. La gluteoplastia mediante lipoinyección debe ser una cirugía segura que partiendo de una planificación adecuada, permita un aumento moderado enfatizando contornos y mejorando la forma natural de la región glútea. Debe permitir obtener resultados predecibles, duraderos y reproducibles, además de ser aplicable en una amplia variedad de pacientes. Presentamos un método de gluteoplastia de aumento sistematizada con lipoinyección que además de ser reproducible, permite obtener resultados consistentes, naturales y permanentes, distribuyendo estratégicamente volúmenes en cuadrantes. Pacientes y Método. Con mínima manipulación del lipoaspirado, infiltramos cantidades controladas en 9 cuadrantes en cada nalga. El cuadrante central representa la zona de máxima proyección y recibe la mitad del volumen. Denominamos zonas primarias a los 4 cuadrantes en los ejes X-Y, zonas que reciben el 40% del volumen infiltrado. Las zonas secundarias o menores corresponden a los cuadrantes situados entre los cuadrantes principales, y reciben el 10% del volumen total. Resultados. Entre 2008 y 2013 intervenimos a 75 pacientes para aumento y remodelación de glúteos con la técnica descrita, todas mujeres de 24 a 52 años. Las pacientes presentaron una convalecencia favorable y una satisfacción del 93%. Nueve pacientes presentaron seromas que se resolvieron mediante aspiración en consultorio. No se presentaron complicaciones mayores. Conclusiones. Presentamos un método de remodelación glútea mediante lipoinyección que, además de ofrecer excelentes resultados, predecibles, consistentes, naturales y permanentes, es lógico y reproducible.

  11. Clasificación automática mediante la CDU con el procedimiento en cadena

    OpenAIRE

    San Segundo Manuel, Rosa

    2002-01-01

    Actas de las I Jornadas de Tratamiento y Recuperación de Información (JOTRI), Valencia, España, 4-5 julio 2002 Se entiende por clasificación automática el proceso de agrupar según el contenido las referencias de los documentos o bien los propios documentos electróneos. Este proceso se realiza mediante programas capaces de comparar términos empleados utilizados en el documento. E incluso hay otras formas automáticas de clasificación que emplean procedimientos auto...

  12. Aprendizaje de procesos analíticos mediante clases prácticas con laboratorios virtuales

    OpenAIRE

    Sierra Fernández, J.; J. Bosque Sendra; García Campaña, A.; Blanc García, R.; Del Iruela, M.; Gámiz Gracia, L.; Alés Barrero, F.

    2009-01-01

    Este trabajo forma parte de un proyecto de innovación docente financiado por la Universidad de Granada durante el curso 2008-09 y pretende que el alumnado del Departamento de Química Analítica de los primeros cursos de Química, Ingeniería Química, Ciencias Ambientales y Farmacia sea capaz de interrelacionar los aspectos teóricos y prácticos de diversos procesos analíticos mediante el uso de laboratorios virtuales en las sesiones prácticas. Con este fin, las clases prácticas son planificadas y...

  13. Clasificación automática mediante la CDU con el procedimiento en cadena

    OpenAIRE

    San Segundo Manuel, Rosa

    2002-01-01

    Actas de las I Jornadas de Tratamiento y Recuperación de Información (JOTRI), Valencia, España, 4-5 julio 2002 Se entiende por clasificación automática el proceso de agrupar según el contenido las referencias de los documentos o bien los propios documentos electróneos. Este proceso se realiza mediante programas capaces de comparar términos empleados utilizados en el documento. E incluso hay otras formas automáticas de clasificación que emplean procedimientos auto...

  14. DESULFURACIÓN DE RELAVE MEDIANTE LA FLOTACIÓN DE SULFUROS DE HIERRO

    OpenAIRE

    Luis Valderrama Campusano; B. Zazzali; J. Chamorro; Santander, M.

    2015-01-01

    En Chile la minería se desarrolla principalmente en  la concentración de cobre, molibdeno, oro y plata, mediante el proceso de flotación. Este genera grandes tonelajes de relaves que contienen diversas minerales, principalmente pirita. Dado que estos minerales se depositan en los tranques, estos pueden generar aguas ácidas; se propone como alternativa la desulfuración de los súlfuros por flotación. En una primera parte se estudió la flotabilidad de la pirita en un tubo Hallimond, utilizando c...

  15. PREDICCION DE PROPIEDADES DE MEZCLAS GASOLINA-ETANOL MEDIANTE ESPECTROSCOPIA DE INFRARROJO

    OpenAIRE

    Felipe Sánchez-Minero; Edgar Ramirez-Jimenez; Guadalupe Silva-Oliver; Berenice Hernandez-Castillejos

    2013-01-01

    El efecto del etanol en la reformulación de una gasolina fue estudiado mediante espectroscopia de infrarrojo (IR). Para ello, se prepararon seis mezclas gasolina-etanol con diferente contenido de alcohol (0, 20, 40, 60, 80 y 100% volumen). Los espectros IR de estas mezclas fueron obtenidos en la región de 4000 a 850 cm -1. A continuación, se determinó un factor característico del etanol (F CO), el cual relaciona el área bajo la curva de la banda a 1050 cm-1 y el área bajo la curva total del e...

  16. Diseño de una banda transportadora mediante guide de Matlab

    OpenAIRE

    Salinero Gervaso, Mario

    2013-01-01

    El objetivo principal del presente proyecto, es la elaboración de un programa para diseñar una banda transportadora mediante GUIDE de MatLab, conforme a las normas: DIN 22101, ISO 5048 y UNE 58204. Otro objetivo (secundario), por el que se ha desarrollado este software, es ayudar a los estudiantes de Ingeniería Industrial en el cálculo y diseño de una cinta transportador, para así facilitarles el entendimiento de parte del temario de la asignatura de "Transportes en la Ingen...

  17. Caracterización de bacterias aisladas en larvas de p.v. mediante rapds

    OpenAIRE

    Cedeño, Ricardo

    2003-01-01

    Caracterización de bacterias aisladas en larvas de P.V. mediante RAPDs La “Vibriosis luminiscente” reportada por varios laboratorios de larvas del Ecuador es asociada principalmente a la bacteria Vibrio harveyi. Los métodos de identificación bacteriana tradicionales se basan en la observación de características morfológicas y pruebas bioquímicas, sin embargo la identificación de especies bacterianas y en especial de Vibrios ambientales ha presentado ciertas dificultades debido al tiempo qu...

  18. PROMOCIÓN DE LA LECTURA E IDENTIDAD DEPORTIVA MEDIANTE TEXTOS DE HISTORIA DEL DEPORTE

    OpenAIRE

    2016-01-01

    En el artículo se expone una experiencia de promoción de la lectura desarrollada mediante un proyecto conjunto de la Facultad de Cultura Física y la biblioteca de la Universidad de Las Tunas. Se fundamenta en aspectos teóricos de la promoción de la lectura y de la identidad deportiva, ambas variables tienen salida práctica en actividades donde confluyen diversas manifestaciones del arte y la literatura con la actividad física y el deporte. El objetivo del trabajo consiste en reflexionar en to...

  19. Terapia de exposición mediante Realidad Virtual en los trastornos alimentarios: presente y futuro

    OpenAIRE

    Ferran Vilalta Abella; Jana Pla Sanjuanelo; Marta Ferrer García; José Gutiérrez Maldonado

    2015-01-01

    El objetivo de este trabajo fue revisar el uso de la realidad virtual (RV) como técnica de exposición para el tratamiento de los trastornos de la conducta alimentaria (TCA). Para ello se analizó la literatura existente con el fin de responder a dos cuestiones: primero, ¿existe evidencia sobre la capacidad de los entornos y estímulos virtuales para provocar respuestas de ansiedad y craving en pacientes con TCA?; y, segundo, ¿la aplicación de técnicas de exposición mediante RV permite la dismin...

  20. Ensayo no destructivo de soldaduras en pernos conectores mediante inspección acústica

    Directory of Open Access Journals (Sweden)

    Aznar, A.

    2012-09-01

    Full Text Available Headed studs are nowadays the standard steel-concrete connectors because of their competitive advantages. Firstly, they provide a high degree of safety thanks to semiautomatic electric arc welding. These welds are not suitable for typical non-destructive tests. The analytical study comprises several models. The first vibration modes have been obtained. The experimental research has developed first the measurement of the natural frequencies of 28 headed-studs in the sonic range. Then they have been tested by non-destructive and destructive tests. Finally theirs tests have been compared with their respective frequency measurements. A clear relationship between the measured frequencies and the lack of penetration of the welds has been established, that confirms the analytical prediction of this effect of the internal weld imperfections. Therefore, the feasibility of simple and absolutely non-destructive tests of welded studs by in site measurement of natural frequencies in the sonic range has been clearly established in this work.

    Los pernos conectores aportan múltiples ventajas de uso, entre las que se encuentra el elevado margen de seguridad que ofrecen sus soldaduras ejecutadas mediante arco eléctrico. Estas soldaduras, aunque ampliamente fiables, son difícilmente comprobadas mediante ensayos no destructivos. El presente estudio plantea la inspección de soldaduras de pernos conectores mediante su espectro acústico. Analíticamente, la investigación se ha centrado en el cálculo de los primeros modos propios de vibración. Experimentalmente se han medido las frecuencias propias de resonancia de 28 pernos, en los que posteriormente se han llevado a cabo ensayos tanto no destructivos como destructivos. Se ha obtenido, tanto teórica como experimentalmente, una relación entre la frecuencia de vibración de los pernos conectores y la calidad de la soldadura. Por ello se verifica la posibilidad de inspección de estas

  1. Tratamiento del tortícolis congénito mediante control estimular y fisioterapia

    OpenAIRE

    MARÍA ASUNCIÓN VÁZQUEZ VILÀ; SUSANA COLLADO VÁZQUEZ

    2004-01-01

    Presentamos un caso clínico de tortícolis congénito y su tratamiento mediante control estimular y fisioterapia, por la alta frecuencia del tortícolis en la práctica clínica y por los buenos resultados que en general presenta el tratamiento conservador en estos casos, principalmente cuando éste se inicia de forma precoz. El caso presentado es de una niña con tortícolis congénito izquierdo, sin patologías asociadas, en tratamiento desde los 15 días hasta los nueve meses de edad, con una buena e...

  2. Determinacion mediante termografía de la reflectancia infrarroja de materiales especulares

    OpenAIRE

    Hongn, Marcos Ezequiel; Flores Larsen, Silvana Elinor

    2017-01-01

    En este trabajo se presenta una metodología experimental destinada a la medición de la reflectancia especular en el rango del IR lejano, mediante la utilización de una cámara termográfica. Las medidas se realizaron para muestras especulares de dos tipos diferentes: con soporte de vidrio y reflectores metálicos. Se estudiaron espejos de distintos espesores y calidad de vidrio, vidrios claros, acrílico y una placa de aluminio. Se determinó la reflectancia infrarroja de estos materiales para dis...

  3. Análisis de corrientes en una red neuronal conectada mediante Gap junctions

    OpenAIRE

    Yustres Díaz, Miguel

    2015-01-01

    La conexión de neuronas mediante sinapsis eléctricas proporciona una mayor velocidad de transmisión sináptica y la capacidad de sincronizar grupos de células para coordinar su actividad. Este tipo de sinapsis permite el intercambio de información continua entre neuronas, no solo cuando se producen potenciales de acción. Dependiendo de la magnitud del acoplamiento, esta sincronización afecta a la actividad sub-umbral y a los potenciales de acción. Un exceso en la sincronización de las neuronas...

  4. Materiales de carbono para desalinización de aguas mediante desionización capacitiva

    OpenAIRE

    Díaz Baizán, Patricia

    2012-01-01

    El presente trabajo muestra los resultados obtenidos al emplear materiales de carbono de elevada área superficial en el proceso de desalinización de aguas mediante desionización capacitiva (CDI). Esta técnica electroquímica, basada en la formación de la doble capa eléctrica en la interfase electrodo/electrolito para retener las sales presentes en el agua tratada, es una alternativa energéticamente menos costosa a las tecnologías comúnmente empleadas como son la ósmosis inversa y la destilació...

  5. Inversion Analytics : Análisis sobre rentabilidad de inversiones mediante VAN y TIR

    OpenAIRE

    Bande Firvida, David

    2010-01-01

    Inversion Analytics es una herramienta para facilitar el análisis en inversiones que se planteen en pequeñas, medianas y grandes empresas, en el que se plasmará toda la información necesaria para determinar los valores que entran en juego en la inversión. La aplicación realiza esta función mediante el cálculo de VAN (Valor Actual Neto) y TIR (Tasa Interna de Rentabilidad) de una inversión determinada, a partir de la información introducida por el usuario y teniendo en cuenta los distintos mét...

  6. Regeneración ósea mediante estímulos mecánicos

    OpenAIRE

    Cocera Castillo, Daniel

    2013-01-01

    RESUMEN: Recientes investigaciones sugieren que la estimulación mecánica del tejido óseo puede provocar la regeneración y el refuerzo del mismo. A partir de esta hipótesis, se han analizado las distintas posibilidades de tratamiento o entrenamiento del hueso mediante técnicas manuales. Entre los resultados más destacables se encuentran como las cargas mecánicas son las responsables de una mejor salud del hueso, siendo capaces de ayudar en la regeneración de fracturas y aumentar la densidad en...

  7. Equipos de trabajo en Contabilidad Financiera para el registro de operaciones mediante documentos reales

    OpenAIRE

    Albelda Pérez, Esther; Florez-Lopez, Raquel

    2012-01-01

    Este trabajo presenta una experiencia de innovación docente mediante la creación de equipos de trabajo para el desarrollo del registro contable de las operaciones más habituales en la práctica empresarial, a través del análisis e interpretación de los documentos reales que justifican dichas operaciones. Esta intervención educativa se puso en práctica durante el segundo cuatrimestre del curso 2008/2009 en la asignatura de Contabilidad Financiera y Analítica del primer curso de la Licenciatura ...

  8. Superación de la dislexia mediante técnicas operantes

    OpenAIRE

    Bernardo Gutiérrez, Inmaculada; Pérez Álvarez, Marino

    1993-01-01

    Numerosos trabajos e investigaciones han tratado de establecer una relación casi causal entre los déficits psicomotrices – lateralidad, orientación, ritmo, etc. – y la dislexia. El presente estudio se dirige a analizar mediante una intervención experimental esta supuesta relación, sobre ta que se sustenta, por otra parte, el tratamiento psicoeducativo tradicional. Se ha aplicado, así, una intervención psicomotriz (paquete terapéutico estándar) y otra conductual (específicamente lectora) a una...

  9. Aprendizaje de procesos analíticos mediante clases prácticas con laboratorios virtuales

    OpenAIRE

    Sierra Fernández, J.

    2009-01-01

    Este trabajo forma parte de un proyecto de innovación docente financiado por la Universidad de Granada durante el curso 2008-09 y pretende que el alumnado del Departamento de Química Analítica de los primeros cursos de Química, Ingeniería Química, Ciencias Ambientales y Farmacia sea capaz de interrelacionar los aspectos teóricos y prácticos de diversos procesos analíticos mediante el uso de laboratorios virtuales en las sesiones prácticas. Con este fin, las clases prácticas son planificadas y...

  10. Conocer el valor de la expresión corporal mediante la danza

    OpenAIRE

    Ochoa Ullate, Garbiñe

    2014-01-01

    Mediante este trabajo se pretende trabajar una serie de actividades y ejercicios para conocer la expresión corporal y la salud a través de de diferentes danzas africanas. Para la realización del trabajo se analizan las principales aportaciones pedagógicas de varios autores relacionados con la enseñanza de estos conceptos. Se trata de un trabajo empírico, con el cual se pretende analizar y estudiar los resultados de la propuesta didáctica para llegar a unas conclusiones acerca de si el ámbi...

  11. Diagnóstico de hiperreactividad bronquial en deportistas mediante el valor del PC20

    OpenAIRE

    Martín-Fernández, M. C.; Melero-Romero, C.; J.R. Alvero-Cruz

    2014-01-01

    En este trabajo se exponen los criterios de positividad del Comité Olímpico Internacional versus a la Sociedad Española de Neumología y Cirugía Torácica. Participaron en este estudio ochenta deportistas de alto rendimiento, realizando una historia clínica, una espirometría de reposo y un test de metacolina. Se analiza la sensibilidad y especificidad del test de metacolina mediante curvas ROC. El comité Internacional antidopaje (WADA) requiere que la disminución sea con un PC20 < a 4mg/ml, ...

  12. Future climate and surface mass balance of Svalbard glaciers in an RCP8.5 climate scenario: a study with the regional climate model MAR forced by MIROC5

    Science.gov (United States)

    Lang, C.; Fettweis, X.; Erpicum, M.

    2015-05-01

    We have performed a future projection of the climate and surface mass balance (SMB) of Svalbard with the MAR (Modèle Atmosphérique Régional) regional climate model forced by MIROC5 (Model for Interdisciplinary Research on Climate), following the RCP8.5 scenario at a spatial resolution of 10 km. MAR predicts a similar evolution of increasing surface melt everywhere in Svalbard followed by a sudden acceleration of melt around 2050, with a larger melt increase in the south compared to the north of the archipelago. This melt acceleration around 2050 is mainly driven by the albedo-melt feedback associated with the expansion of the ablation/bare ice zone. This effect is dampened in part as the solar radiation itself is projected to decrease due to a cloudiness increase. The near-surface temperature is projected to increase more in winter than in summer as the temperature is already close to 0 °C in summer. The model also projects a stronger winter west-to-east temperature gradient, related to the large decrease of sea ice cover around Svalbard. By 2085, SMB is projected to become negative over all of Svalbard's glaciated regions, leading to the rapid degradation of the firn layer.

  13. Ahorro energético mediante estrategias de iluminación natural optimizadas

    Directory of Open Access Journals (Sweden)

    Puigdomènech Franquesa, Joan

    1986-04-01

    Full Text Available Electrical charges in buildings and specially in those of commercial use, can be diminished by means of natural lighting strategies. Taking the climate features of our country into consideration, it is necessary to prevent the inconveniences caused by an en erg y excess in summer, so solar Controls are needed. The only practical way to achieve the suitable balance between thermal and light needs, so as to get a monthly or annual energetic balance optimization, is to operate with the computer. A programme with such characteristics is described here. Its application gives important sarings in non renouvable energy savings.Mediante estrategias de iluminación natural es posible disminuir las cargas eléctricas de los edificios y en especial los de uso comercial. Dadas las características climáticas de nuestro país es necesario prever los inconvenientes de un exceso de energía en verano, para lo cual es preciso disponer de controles solares. Encontrar el correcto equilibrio entre las necesidades térmicas y lumínicas en base a la optimización del balance energético mensual o anual es únicamente factible mediante el uso del ordenador. Un programa que responde a estas características es descrito en el presente trabajo, obteniéndose con su aplicación importantes ahorros en el consumo de energías no renovables.

  14. Pronósticos de inflación mediante técnicas bayesianas

    Directory of Open Access Journals (Sweden)

    Juan Diego Chavarría

    2015-11-01

    Full Text Available La efectividad de la política monetaria bajo un esquema de metas de inflación como el propuesto por el Banco Central de Costa Rica se basa en buena medida en el correcto y oportuno pronóstico de la inflación a corto y mediano plazo con el fin de diseñar de mejor forma las acciones de política monetaria. Así, el propósito de este trabajo es desarrollar una herramienta complementaria para elaborar pronósticos de inflación mediante un enfoque bayesiano. Para lo anterior se propone la utilización de la metodología Bayesian Model Averaging y de Weighted Average Least Squares. Los modelos de proyección especificados permitirían ampliar y complementar el análisis que se realiza actualmente con el Modelo Macroeconómico de Proyección Trimestral (MMPT del Banco Central de Costa Rica. Como resultado esta investigación muestra que, para datos de periodicidad mensual y a horizontes de pronóstico de 1 a 12 meses, es posible encontrar proyecciones mediante un proceso bayesiano que poseen una mayor capacidad predictiva en relación con aquellas producidas por un modelo autorregresivo.

  15. Principios básicos y aplicación del aprendizaje mediante tareas

    Directory of Open Access Journals (Sweden)

    Estaire, Sheila

    2011-04-01

    Full Text Available En los últimos años el aprendizaje mediante tareas ha ido consolidándose como una nueva forma de enseñar y aprender lenguas extranjeras. Sin embargo existen una serie de aspectos prácticos relacionados con su aplicación en los que aún se puede profundizar. En este artículo, después de una introducción breve de algunos principios básicos, se discuten posibles procedimientos para determinar las tareas que constituyen el eje de un programa, así como para organizar el proceso de enseñanza / aprendizaje. A continuación se presentan diferentes modalidades de trabajo sobre los aspectos formales de la lengua, aspectos que es esencial tratar de forma rigurosa, minuciosa y sistemática. Este punto crucial se discute junto con una propuesta de estructura de curso que consta de dos componentes diferenciados. Por otra parte, los elementos innovadores del aprendizaje mediante tareas hacen imprescindible una gestión del aprendizaje también innovadora, aspecto que se trata en el último apartado a través de pautas metodológicas que potencian la eficacia de las tareas como instrumento de aprendizaje.

  16. Graduación mediante funciones Gompertz-Makeham dinámicas.

    Directory of Open Access Journals (Sweden)

    Montes Suay, Francisco

    2004-01-01

    Full Text Available El objetivo de este trabajo es modelar la influencia del tiempo de calendario y la edad, en las probabilidades de muerte, qx. Para ello, se gradúa los datos de mortalidad de la Comunidad Valenciana correspondientes a los años 1980 a 1999. En primer lugar obtenemos el ajuste de las funciones Gompertz-Makeham con términos correspondientes a las variables edad y tiempo mediante modelos lineales generalizados. La elección del modelo que mejor se ajusta a los datos de cada periodo se lleva a cabo atendiendo a criterios de bondad de ajuste y de complejidad del mismo. Se trata de buscar un equilibrio entre ambos aspectos, para lo cual utilizamos medidas y contrastes implementados en S-plus. Del modelo obtenido para cada sexo hacemos un análisis más detallado mediante los tests no paramétricos habituales y análisis gráfico, para concluir la adecuación de éstos a la experiencia de mortalidad.

  17. PROPUESTA DE CONEXIÓN DE ENTORNOS IPv6 MEDIANTE UN BACKBONE MPLS/IPv4

    Directory of Open Access Journals (Sweden)

    Nancy Yaneth Gelvez García

    2013-09-01

    Full Text Available Las redes actuales MPLS/IPv4 presentan las ventajas de poder implementar ingeniería de tráfico, así como realizar diferenciación de flujos mediante clases de servicio (CoS frente a las redes con enrutamiento IP tradicional. En aras de aprovechar cualidades estratégicas durante la etapa de coexistencia entre IPv4 e IPv6 existen 4 métodos para proveer conectividad a islas IPv6 [1] remotas a través de una infraestructura de core MPLS con IPv4 nativo [2], sin embargo una de las formas que permite un rápida y fácil provisión de la misma dados los mínimos requisitos de configuración y de equipos es la de disponer túneles IPv6 en los enrutadores de acceso (CE de la red. No obstante, sus cuatro variantes (manual, GRE, 6to4 e IPv6 compatible IPv4 [3] resultan adecuadas o no según las características inherentes de la red a interconectar; por tanto este artículo presenta las ventajas y desventajas propias de la utilización de cada técnica de entunelamiento como resultado de la interconexión con los cuatro tipos de túneles de una red emulada mediante GNS3+Dynamips.

  18. Introducción de momentos internos de torsión mediante pretensado

    Directory of Open Access Journals (Sweden)

    Johannson, J.

    1975-02-01

    Full Text Available In this article the possibility is being studied of transferring bending moments to the ground by means of torsional strength of a beam. This avoids the need to subject columns to high torsional stresses. This system, by prestressing, induces internal moments which compensate the torsional moment. This reduces, or eliminates, under certain loading cases, the angular deflection due to torsion couples. Primarily, a study is carried out on this type torsion — with the example of a simple hollow beam, in corbel,En este artículo se estudia la posibilidad de transferir momentos al firme mediante la resistencia torsional de una viga, evitándose así soluciones de pilas o pilares solicitados fuertemente por torsión y creando, mediante pretensado, momentos internos que contrarresten directamente al momento torsor actuante, con lo cual se reduce e incluso se elimina, para ciertos casos de carga, el giro de la sección debido al par torsor. En primer lugar se lleva a cabo un estudio de este tipo de torsión con el ejemplo sencillo de una viga hueca, en voladizo, solicitada en un extremo por un par torsor.

  19. Obtención de electricidad y agua potable mediante energía solar para un centro de salud mozambiqueño

    OpenAIRE

    Antona Díaz, Ana María

    2009-01-01

    El proyecto consiste en cubrir las necesidades de energía eléctrica y agua potable del centro de salud de Minehuene (Mozambique), mediante energía solar fotovoltaica y térmica. La cobertura de la demanda de electricidad del centro se realiza mediante un sistema de energía fotovoltaico. Para la obtención de agua potable se evalúan diferentes alternativas: pasteurización del agua de lluvia mediante paneles solares térmicos, purificación de esta agua mediante lámparas ultravioletas u obtenci...

  20. Selección de estaciones termométricas mediante un filtro de comparación de curvas.

    OpenAIRE

    José Antonio Sosa Cardo

    2001-01-01

    A la hora de realizar cualquier estudio climatológico o interpolación espacial es fundamental contar con un conjunto de estaciones fiables, y desechar aquellas que lo único que nos van a aportar es ruido. Se han desarrollado dos métodos para seleccionar estaciones de forma objetiva: 1) Mediante análisis de las formas de las curvas de las series, siendo necesaria otra estación con una curva similar para que se de por válida una estación. 2) Mediante análisis del valor diario. Cuando existe una...

  1. Detección proviral de HTLV-1 mediante reacción en cadena de la polimerasa (PCR)

    OpenAIRE

    Sevilla, Carlos; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Alarcón, Jorge; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Huiza, Alina; D. A. Microbiología Médica, Facultad de Medicina, UNMSM; Centro de Biotecnología, Universidad Iberoamericana de Ciencias y Tecnología; Laboratorio Entomología Médica, Sección Parasitología, Instituto de Salud Pública; Laboratorio de Entomología Médica, Laboratorio de Referencia Regional, Gerencia Regional de Salud La Libertad; Dirección Ejecutiva de Salud Ambiental, Sub Región de Salud “Luciano Castillo Colonna”, DIRESA Piura.; Gutiérrez, César; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Ñavincopa, Marcos; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Cornejo, William; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Cáceres, Abraham; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Tejada, Abelardo; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Romaní, Franco; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Mori, Nicanor; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Wong, Paolo; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Huamán, Víctor; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Vásquez, Rubén; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Tejada, Romina; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Flores, Alitza; D.A. Microbiología Médica, Facultad de Medicina, UNMSM

    2013-01-01

    Objetivos: Detectar el genoma proviral de HTLV-1 mediante el desarrollo de reacción en cadena de la polimerasa (PCR). Diseño: Descriptivo. Institución: D.A. Microbiología Médica, Facultad de Medicina, UNMSM. Participantes: Personas con y sin sospecha de HTLV-I. Principales medidas de resultados: detección de HTLV-1 mediante PCR. Resultados: El 71,4% de los pacientes con sospecha clínica de HTLV-I fue reactivo por métodos Inmunológico. Elisa HTLV I-II Biokit detectó 5 casos reactivos (X=2,359 ...

  2. Mapas de Entornos Mediante Navegacion Difusa y Sistema de Teleoperacion de una Plataforma Pioneer P3-DX

    Directory of Open Access Journals (Sweden)

    Daniel Granda

    2013-11-01

    Full Text Available El presente proyecto describe el diseno e implementacion de aplicaciones de Teleoperacion, Adquisicion de Datos, Control Difuso de Velocidad y Mapeo de Entornos en 2D, para la plataforma movil Pioneer P3-DX mediante el uso de sonares, odometrıa y software libre GNU/Linux. El proyecto brinda una guıa para utilizar los conceptos de programacion en Python, que permite crear aplicaciones de manera versatil mediante el uso de librerıas como: GTK para el desarrollo del entorno grafico, PYFUZZY para el desarrollo del controlador difuso de velocidad y OPENCV para mostrar los mapas del entorno.

  3. Remoción de Contaminantes Atmosféricos Sox y Nox mediante Nanotubos de Carbono

    OpenAIRE

    Saavedra Labastida, Erika

    2013-01-01

    En el presente proyecto de investigación se realizó la evaluación de la remoción de los contaminantes atmosféricos provenientes de fuentes móviles, mediante nanotubos de carbono (NTC) comerciales. Para conocer las propiedades de los NTC empleados, fueron caracterizados fisicoquímicamente mediante diferentes análisis tales como MEB, MET, Raman, DRX y BET, los resultados que se obtuvieron mostraron óptimas propiedades fisicoquímicas las cuales permiten que sean empleados como ...

  4. Detección proviral de HTLV-1 mediante reacción en cadena de la polimerasa (PCR)

    OpenAIRE

    Sevilla, Carlos; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Alarcón, Jorge; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Huiza, Alina; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Gutiérrez, César; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Ñavincopa, Marcos; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Cornejo, William; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Cáceres, Abraham; D. A. Microbiología Médica, Facultad de Medicina, UNMSM; Centro de Biotecnología, Universidad Iberoamericana de Ciencias y Tecnología; Laboratorio Entomología Médica, Sección Parasitología, Instituto de Salud Pública; Laboratorio de Entomología Médica, Laboratorio de Referencia Regional, Gerencia Regional de Salud La Libertad; Dirección Ejecutiva de Salud Ambiental, Sub Región de Salud “Luciano Castillo Colonna”, DIRESA Piura.; Tejada, Abelardo; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Romaní, Franco; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Mori, Nicanor; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Wong, Paolo; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Huamán, Víctor; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Vásquez, Rubén; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Tejada, Romina; D.A. Microbiología Médica, Facultad de Medicina, UNMSM; Flores, Alitza; D.A. Microbiología Médica, Facultad de Medicina, UNMSM

    2013-01-01

    Objetivos: Detectar el genoma proviral de HTLV-1 mediante el desarrollo de reacción en cadena de la polimerasa (PCR). Diseño: Descriptivo. Institución: D.A. Microbiología Médica, Facultad de Medicina, UNMSM. Participantes: Personas con y sin sospecha de HTLV-I. Principales medidas de resultados: detección de HTLV-1 mediante PCR. Resultados: El 71,4% de los pacientes con sospecha clínica de HTLV-I fue reactivo por métodos Inmunológico. Elisa HTLV I-II Biokit detectó 5 casos reactivos (X=2,359 ...

  5. Prokaryotic expression of Leptospira interrogans FliH/Ⅰ/Y/N genes and determination of the protein locations in leptospiral envelope%问号钩端螺旋体鞭毛蛋白相关蛋白FliH/Ⅰ/Y/N基因原核表达和膜定位

    Institute of Scientific and Technical Information of China (English)

    徐韩飞; 阮萍; 廖苏梅; 杨平; 毛亚飞; 李立伟; 严杰

    2008-01-01

    目的 克隆问号钩端螺旋体(简称钩体)鞭毛相关蛋白编码jliH、fliⅠfliH、和fliN并构建其原核表达系统,制备表达产物抗血清并了解其蛋白的定位.方法 以苯酚-氯仿法提取的问号钩体黄疸出血群赖型赖株基凶组DNA为模板,用PCR扩增全长fliH,fliⅠ,firY和flfliN基因片段,T-A克隆后测序,继而构建上述目的 基因克隆的原核表达系统.采用SDS-PAGE和BioRad凝胶图像分析系统检查重组蛋白rFliH、rFliI、rFliY和rHiN的表达情况,Ni-NTA亲和层析法提纯目的 表达产物.皮下免疫家兔获得4种目的 重组蛋白抗血清,用ELISA和Western blot分别检测抗血清效价并了解抗血清与相应抗原结合的能力.采用免疫电镜技术对FliH、FliⅠ、FliY和FliN进行定位.结果 PCR扩增获得大小分别为924、1365、1065和318 bp的全长fliH,fliⅠ,fliY和fliN基因片段,与报道的序列比较.其核苷酸和氨基酸序列相似性均为100%.所构建的原核表达系统均能有效地表达目的 重组蛋白,其产量均约为20%.rFliH、rFliⅠ、rFliY和rHiN蛋白免疫家兔后能产生抗体,其兔抗血清ELISA效价达到1:100 000以上,并分别识别钩体相应重组蛋白和膜蛋白提取物而出现明显的Westem杂交条带.FiH、FliⅠ、FliY和FliN蛋白分布于问号钩体内膜、外膜或内外膜之间.结论 本研究成功地构建了能高效表达问号钩体鞭毛相关蛋白FliH、FliⅠ、FIiY和HiN的原核表达系统,并获得了能有效识别上述蛋白抗原的高效价抗血清.鞭毛相关蛋白HiH、Flil、FliY和FIiN是问号钩体内膜或外膜蛋白成分.%Objective To clone fliH, fliⅠ, fliY and fliN genes that encoding flagellum-associated proteins of L. interrogans for construction of their prokaryotic expression systems, and to determine the loca- tions of Flirt, FliⅠ, FIiY and FIiN. Methods The fliH, fliⅠ, fliY andfliN genes were amplified by PCR and sequenced after T-A cloning. Prokaryotie

  6. Expression pattern and apoptosis-inducing activity to murine macrophages and hepatocytes of Leptospira interrogans Sph2 hemolysin%钩端螺旋体溶血素Sph2表达模式及诱导巨噬细胞和肝细胞凋亡活性

    Institute of Scientific and Technical Information of China (English)

    丁士标; 林旭瑷; 王欢; 严杰

    2010-01-01

    目的 了解问号钩端螺旋体(简称钩体)感染细胞前后sph2基因表达水平变化,确定鞘磷脂酶类溶血素Sph2及诱导细胞凋亡的活性.方法 采用PCR从黄疸出血群赖型赖株钩体基因组DNA中扩增全长sph2基因片段,T-A克隆后测序.构建sph2基因原核表达系统,采用SDS-PAGE检查重组Sph2(rSph2)的表达情况,Ni-NTA亲和层析法提纯rSph2.采用绵羊血平板溶血试验及血红蛋白分光光度法测定rSph2的溶血活性.采用流式细胞术检测rSph2诱导小鼠单核-巨噬样细胞株J774A.1和肝细胞株IAR20凋亡的活性,实时荧光定量PCR检测赖株钩体感染J774A.1和IAR20细胞前后sph2基因mRNA水平变化.结果 与GenBank中sph2基因比较,所克隆的sph2基因序列相似性为100%.所构建的原核表达系统能高效表达rSph2.rSph2以浓度依赖方式溶解绵羊红细胞.10 μg/ml rSph2可诱导J774A.1和IAR20细胞凋亡,凋亡率峰值分别为23.96%和32.92%.赖株钩体感染J774A.1和IAR20细胞后0.5~2 h内sph2基因mRNA水平显著升高,2 h后mRNA水平迅速下降.结论 钩体sph2基因呈宿主细胞接触式瞬时表达.rSph2有溶解绵羊红细胞及诱导巨噬细胞和肝细胞凋亡的活性,因而Sph2是钩体致病过程中重要的毒力因子.%Objective To determine the change of expression level of Leptospira interrogans sph2 gene, and hemolytic and cell apoptosis-inducing activities of sphingomyelinase hemolysin Sph2. Methods Entire sph2 gene fragment was amplified by PCR from genomic DNA of L. Interrogans serovar serogroup Icterohaemorrhagiae serovar Lai strain Lai, and sequenced after T-A cloning. Subsequently, a prokaryotic expression system of sph2 gene was constructed. The expression of target recombinant Sph2( rSph2 ) was examined by SDS-PAGE and the expressed rSph2 was extracted by Ni-NTA affinity chromatogaphy. The hemolytic activity of rSph2 was measured by hemolytic test in sheep blood agar plate and spectrophotometry-based hemoglobin

  7. Impact of climate change on Precipitation and temperature under the RCP 8.5 and A1B scenarios in an Alpine Cathment (Alto-Genil Basin,southeast Spain). A comparison of statistical downscaling methods

    Science.gov (United States)

    Pulido-Velazquez, David; Juan Collados-Lara, Antonio; Pardo-Iguzquiza, Eulogio; Jimeno-Saez, Patricia; Fernandez-Chacon, Francisca

    2016-04-01

    In order to design adaptive strategies to global change we need to assess the future impact of climate change on water resources, which depends on precipitation and temperature series in the systems. The objective of this work is to generate future climate series in the "Alto Genil" Basin (southeast Spain) for the period 2071-2100 by perturbing the historical series using different statistical methods. For this targeted we use information coming from regionals climate model simulations (RCMs) available in two European projects, CORDEX (2013), with a spatial resolution of 12.5 km, and ENSEMBLES (2009), with a spatial resolution of 25 km. The historical climate series used for the period 1971-2000 have been obtained from Spain02 project (2012) which has the same spatial resolution that CORDEX project (both use the EURO-CORDEX grid). Two emission scenarios have been considered: the Representative Concentration Pathways (RCP) 8.5 emissions scenario, which is the most unfavorable scenario considered in the fifth Assessment Report (AR5) by the Intergovernmental Panel on Climate Change (IPCC), and the A1B emission scenario of fourth Assessment Report (AR4). We use the RCM simulations to create an ensemble of predictions weighting their information according to their ability to reproduce the main statistic of the historical climatology. A multi-objective analysis has been performed to identify which models are better in terms of goodness of fit to the cited statistic of the historical series. The ensemble of the CORDEX and the ENSEMBLES projects has been finally created with nine and four models respectively. These ensemble series have been used to assess the anomalies in mean and standard deviation (differences between the control and future RCM series). A "delta-change" method (Pulido-Velazquez et al., 2011) has been applied to define future series by modifying the historical climate series in accordance with the cited anomalies in mean and standard deviation. A

  8. Representación del Conocimiento en curriculo mediante esquemas preconceptuales

    Directory of Open Access Journals (Sweden)

    Carlos Mario Zapata

    2011-09-01

    Full Text Available El concepto de currículo se torna más y más complejo en tanto aparecen nuevos estudios que lo complementan. Como consecuencia, los modelos que, gráfica o formalmente, tratan de representar el conocimiento alrededor del currículo se ocupan cada vez más de aspectos locales, de este modo le restan generalidad de comprensión. Por ello, en este artículo de investigación se realiza una revisión acerca de los diferentes enfoques del currículo a lo largo del siglo XX y de los modelos que representan este concepto. Finalmente, se propone una representación integradora de las diferentes visiones de currículo mediante los denominados esquemas preconceptuales, que consisten en diagramas para la representación del conocimiento cercanos al lenguaje natural

  9. Predictor de Smith modificado mediante un modelo interno, robusto a perturbaciones externas no medibles.

    Directory of Open Access Journals (Sweden)

    Fernando Castillo García

    2010-11-01

    Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4 Se propone una modificación de la estructura del predictor de Smith mediante un modelo interno que posibilita aumentar su rechazo al efecto de las per-turbaciones externas no medibles en comparación con la estructura clásica del predictor de Smith. Los resultados obtenidos se aplican en el diseño de un sistema de control del proceso de variación de la temperatura del jugo en los calentadores de una fábrica de azúcar. Los resultados de la simulación del sistema diseñado mostraron su efectividad y robustez en cuanto al rechazo de perturbaciones externas no medibles. Palabras claves: Predictor de Smith, predictor de Smith modificado, rechazo a perturbaciones externas no medibles, robustez de los sistemas de control.

  10. Evaluando impactos externos mediante un modelo de equilibrio general computable con competencia imperfecta: El caso colombiano

    Directory of Open Access Journals (Sweden)

    Diana Catalina Gutiérrez

    2008-10-01

    Full Text Available Mediante un modelo de equilibrio general computable, en el que se discrimina un sector informal en la economía, se modela el sector formal como un mercado de competencia monopolística. Además de incluir mercados de trabajo discriminados, este modelo también evalúa el impacto del creciente flujo de inversión extranjera, del aumento de precio de los bienes básicos, y del aumento del volumen de las exportaciones sobre la economía colombiana. Se concluye que algo más de la mitad del crecimiento observado puede explicarse por el efecto de los factores mencionados. No obstante, se señala la importancia de las políticas internas para aprovechar las coyunturas favorables, y para minimizar el efecto de circunstancias negativas sobre el desempeño económico.

  11. Variabilidad genética de Hartón del Valle mediante RAM

    Directory of Open Access Journals (Sweden)

    Ana M Piedrahíta

    2008-01-01

    Full Text Available El Hartón del Valle (HV hace parte de las siete razas criollas de ganado bovino colombiano y de acuerdo con los criterios de la FAO está considerado como vulnerable. Para estudiar su variabilidad genética fueron muestreados 33 individuos HV y tres animales de la raza Holstein, como control externo. Se extrajo ADN utilizando el método de Salting Out y las muestras fueron analizadas mediante la técnica molecular RAM (Randon amplified microsatellites. Se utilizaron los cebadores CGA, CCA, TG, y CT. El valor promedio de heterocigosidad esperada fue de 0.26 y el F ST fue 0.39 ± 0.03. Con el índice de Dice-Nei Li y agrupando con el método UPGMA se distinguieron dos grupos: uno integrado por dos hatos de conservación y el otro por cuatro fincas que han compartido reproductores.

  12. PROMOCIÓN DE LA LECTURA E IDENTIDAD DEPORTIVA MEDIANTE TEXTOS DE HISTORIA DEL DEPORTE

    Directory of Open Access Journals (Sweden)

    José Guillermo Montero Quesada

    2016-04-01

    Full Text Available En el artículo se expone una experiencia de promoción de la lectura desarrollada mediante un proyecto conjunto de la Facultad de Cultura Física y la biblioteca de la Universidad de Las Tunas. Se fundamenta en aspectos teóricos de la promoción de la lectura y de la identidad deportiva, ambas variables tienen salida práctica en actividades donde confluyen diversas manifestaciones del arte y la literatura con la actividad física y el deporte. El objetivo del trabajo consiste en reflexionar en torno a la necesidad de asumir alternativas de cómo influir en la formación integral de los estudiantes universitarios de la carrera de Cultura Física, con énfasis en los conocimientos históricos y culturales.

  13. Introducción al cálculo mediante resolución de problemas

    OpenAIRE

    Espinoza, Johan; Zumbado, Marianela

    2010-01-01

    Esta investigación presenta la puesta en práctica de una propuesta pedagógica para apoyar la enseñanza del Cálculo mediante la resolución de problemas a nivel preuniversitarioen Costa Rica. El proyecto tiene su origen en las dificultades que presentan los estudiantes en la comprensión de conceptos básicos de Cálculo, específicamente el de límite y derivada. Esta experiencia se fundamentó en la elaboración de una “situación problema” que provocó un conflicto intelectual en los estud...

  14. Herramienta e-learning para la programación de robots mediante entorno web

    Directory of Open Access Journals (Sweden)

    Alfredo Rosado Muñoz

    2008-12-01

    Full Text Available Este artículo describe los trabajos realizados para permitir a los estudiantes de diversas titulaciones de ingeniería la realización de prácticas a distancia en un laboratorio remoto desde un navegador web. Este hecho posibilita utilizar aparatos y maquinaria a distancia (inicialmente un robot industrial, pudiendo programarlos y visualizar el resultado de la programación realizada en tiempo real así como interactuar con la máquina. Adicionalmente, se propone al estudiante la realización de un curso de programación mediante diversas prácticas que debe realizar y el sistema web autoevalúa. Este trabajo ha requerido la integración de diversos elementos hardware y software

  15. Comparación del gasto energético en reposo determinado mediante calorimetría indirecta y estimado mediante fórmulas predictivas en mujeres con grados de obesidad I a III

    Directory of Open Access Journals (Sweden)

    Alicia Parra-Carriedo

    2013-04-01

    Full Text Available Introducción: La determinación del gasto energético en reposo (GER se calcula cotidianamente a partir de fórmulas predictivas aunque el resultado varía dependiendo de la población. Objetivo: Comparar la determinación del GER mediante calorimetría indirecta y mediante las ecuaciones Harris-Benedict (HB, Mifflin (MF, Organización Mundial de la Salud (OMS, "Institute of Medicine" (IOM, Fórmula Rápida (FR y Valencia (VA en mujeres con grados de obesidad I a III. Métodos: Mujeres adultas mestizas mexicanas se incluyeron en el estudio y formaron 3 grupos a partir del índice de masa corporal (IMC. Obesidad grado I (IMC 30,034,9; n = 42, grado II (IMC 35,0-39,9; n = 38 y grado III (IMC > 40; n = 41. Se determinó el GER mediante calorimetría indirecta y mediante las ecuaciones antes señaladas. ANOVA de Kruskal-Wallis y la prueba de Dunn's se emplearon para el análisis estadístico (p 30. En el grado III VA y FR muestran el mejor desempeño.

  16. Uso della chemiluminescenza per la diagnosi della brucellosi bovina e ovina mediante ELISA indiretta e competitiva

    Directory of Open Access Journals (Sweden)

    Manuela Tittarelli

    2008-06-01

    Full Text Available I metodi ufficiali previsti dal Piano nazionale di eradicazione della brucellosi bovina ed ovi-caprina sono la sieroagglutinazione rapida (SAR con l’antigene acidificato al Rosa Bengala e la fissazione del complemento (FDC. Nella attuale fase del piano di eradicazione non è infrequente imbattersi in risultati di difficile interpretazione ottenuti con i test ufficiali, pertanto è necessario poter disporre di test aggiuntivi che presentino livelli di specificità e di sensibilità più elevati. A questo scopo sono stati validati due metodi ELISA, indiretto (i-ELISA CL e competitivo (c-ELISA CL, mediante l’utilizzo di un substrato chemiluminescente per la determinazione di anticorpi anti-Brucella in siero bovino ed ovino. I metodi si basano sulla rivelazione degli anticorpi anti-Brucella, contenuti nel siero, mediante la catalisi di un substrato enzimatico chemiluminescente (sistema luminolo/H2O2/ enhancer, da parte della perossidasi coniugata ad anticorpi secondari anti-IgG, nella i-ELISA CL, o al monoclonale anti-LPS, nella c-ELISA CL. Sulla base dei risultati ottenuti, per l'i-ELISA CL è stato stabilito un valore di cut-off, espresso come percentuale di positività (PP, del 60% per i sieri bovini e del 37,5% per quelli ovini; con questo valore di cut-off si ottiene una sensibilità ed una specificità del test del 100% per i sieri bovini, e una sensibilità del 100% e una specificità del 99,8% per quelli ovini. Per la c-ELISA CL è stato scelto un cut-off, espresso come percentuale di inibizione (PI, del 30% per i sieri bovini e del 40% per quelli ovini, che assicura valori di sensibilità e specificità del 100% in entrambi i casi.

  17. Identificación en lazo cerrado mediante algoritmos genéticos

    Directory of Open Access Journals (Sweden)

    Alfredo Gómez Infante

    2010-11-01

    Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4 st1:*{behavior:url(#ieooui } /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;} La identificación  en lazo cerrado de sistemas continuos es un problema de optimización no lineal que resulta de difícil solución mediante métodos convencionales.  En este trabajo se propone el uso de algoritmos genéticos (AG para esta tarea y se muestra mediante simulaciones que los modelos obtenidos pueden reproducir fielmente el comportamiento de la planta, aun en el caso de sistemas inestables o que presentan algún tipo de no-linealidad. Estos modelos pueden utilizarse para el ajuste de los parámetros de reguladores PID utilizando un método también basado en AG, como se muestra en dos ejemplos: un sistema inestable en lazo cerrado y otro de alto orden con retardo.  La metodología propuesta se aplica también  a la identificación del modelo AC5A de la IEEE, que incluye una no-linealidad del tipo saturación,  para unidades de generación eléctrica.

  18. Estabilización de Suelos mediante el empleo de Sales Cuaternarias

    Directory of Open Access Journals (Sweden)

    Juan M. Junco del Pino

    2010-01-01

    Full Text Available El Mundo se dirige hacia el aprovechamiento de los Suelos mediante el desarrollo de nuevas técnicas y adaptarse a las condiciones del entorno resulta importante para la Ingeniería. El mejoramiento de los suelos abre nuevas posibilidades de ahorro que pueden llegar de 20 a 45 % respecto a los costos de construcción convencional. La Estabilización Química de Suelos consiste en el empleo de sustancias químicas con el objetivo de modificar las propiedades del suelo para hacerlo más denso o incrementar la vinculación de las partículas del mismo para aumentar su capacidad de soporte sin deformación. Las formas más empleadas en la Estabilización Química de Suelos son con cemento, sales y cal. A partir de estas necesidades se desarrolló una Investigación en Cuba que culminó con la creación de un Sistema de Estabilización e Impermeabilización de Suelos a partir del empleo de Sales Cuaternarias, el cual tiene como efectos : Economía, Resistencia, Simplicidad y beneficios ecológicos. Las Aplicaciones fundamentales de esta Técnica de Estabilización e Impermeabilización son en Subrasantes estabilizadas para pavimentos, Mantenimiento y Construcción de Vías, Terraplenes para ferrocarril y Basureros, entre otros. Nuestro trabajo abarca parte de los Resultados logrados mediante la aplicación de este Sistema de Estabilización e Impermeabilización, mostrando como su efecto sobre las Propiedades Mecánicas y de Impermeabilidad en los Suelos aumentan con el tiempo.

  19. 360. Implante de la Prótesis Aórtica Perceval s Mediante Minitoracotomía Derecha

    Directory of Open Access Journals (Sweden)

    2012-04-01

    Conclusiones: en nuestra experiencia la Perceval S® es una prótesis sin sutura que por su hemodinámica y facilidad de implante la convierten en una prótesis indicada para su utilización en raíces aórticas pequeñas mediante acceso miniinvasivo.

  20. Análisis competitivo mediante grupos estratégicos: aplicación al sector hotelero alicantino

    OpenAIRE

    Pereira-Moliner, Jorge

    2004-01-01

    Este documento de trabajo pretende reflejar, de forma resumida, los resultados obtenidos en el proyecto de investigación titulado “Análisis competitivo mediante grupos estratégicos: aplicación al sector hotelero alicantino” que fue realizado en el Departamento de Organización de Empresas de la Universidad de Alicante en 2004.

  1. Superficie específica de una bentonita mediante la adsorción de azul de metileno

    OpenAIRE

    2010-01-01

    Se estudió la determinación de la superficie específica de una bentonita colombiana, procedente del Valle del Cauca, mediante la adsorción de azul de metileno, a 298 K. Este método se comparó con el de la adsorción de nitrógeno a 77 K.

  2. RCP Local School Projects in Alabama.

    Science.gov (United States)

    Regional Curriculum Project, Atlanta, GA.

    One of 6 state reports generated by the Regional Curriculum Project (funded under the Elementary and Secondary Education Act), the document describes 4 specific projects implemented through the Alabama State Superintendent's Office beginning in 1966. All 4 projects were designed to improve instructional leadership by defining the role(s) of the…

  3. RCP Local School Projects in North Carolina.

    Science.gov (United States)

    Regional Curriculum Project, Atlanta, GA.

    One of 6 state reports prepared in cooperation with the Regional Curriculum Project, the document discusses 4 major educational programs conducted in North Carolina since 1965. "The Story of Merger and Educational Change in Moore County" is a report relating to school redistricting; "The Mathematics Project in Greensboro"…

  4. RCP Local School Projects in South Carolina.

    Science.gov (United States)

    Regional Curriculum Project, Atlanta, GA.

    One of 6 state reports of projects and programs operating in cooperation with the Regional Curriculum Project, the document highlights major curriculum-change programs in South Carolina which were initiated in 1966. The 4 projects reported are "Curriculum Study in Berkeley County," which had as its purpose the identification and…

  5. El desarrollo de competencias transversales en dirección de operaciones mediante proyectos de emprendimiento

    Directory of Open Access Journals (Sweden)

    Eva Martínez-Caro

    2012-11-01

    Full Text Available Propósito: En este trabajo se propone el desarrollo de proyectos de emprendimiento con el triple objetivo de acercar al alumno a la Dirección de Operaciones (DO mediante un aprendizaje activo, facilitar la adquisición de competencias transversales, y atender a la creciente demanda de la sociedad de fomentar el espíritu emprendedor.Trabajo relacionado: Tradicionalmente, las asignaturas se han orientado al desarrollo y evaluación de las competencias específicas, dejando en un segundo plano las competencias transversales. Sin embargo, en el contexto del Espacio Europeo de Educación Superior (EEES, ambas competencias deben ser desarrolladas. En particular, en el campo de la Dirección de Operaciones es manifiesta la necesidad de emplear nuevos métodos docentes que complementen las presentaciones teóricas, aproximen a los estudiantes a la compleja realidad de la DO de manera más práctica y faciliten la adquisición de competencias transversales por parte de los alumnos. Los proyectos de emprendimiento pueden ser una herramienta para alcanzar esos objetivos a la vez que promueven una cultura emprendedora que es fundamental para el desarrollo y la competitividad de un país.Diseño/Metodología: Se realizó una experiencia piloto que tenía como objetivo integrar en una asignatura de Ingeniería Industrial dedicada tradicionalmente al desarrollo de competencias específicas del campo de DO, competencias transversales mediante el desarrollo de proyectos de emprendimiento empresarial. Los resultados se midieron mediante una encuesta suministrada a los 60 alumnos de la asignatura.Resultados: Los resultados señalan que la elaboración de este tipo de proyectos ha ayudado a los alumnos a desarrollar y a adquirir de manera óptima la mayoría de las competencias transversales. El grado de satisfacción de los alumnos con la experiencia ha sido muy alto.Implicaciones: Este trabajo contribuye a la literatura sobre docencia en DO aportando un m

  6. Recubrimientos de TiN depositados mediante ACPVD sobre aleaciones de magnesio AM60

    Directory of Open Access Journals (Sweden)

    Pichel, M.

    2013-06-01

    Full Text Available Magnesium alloys are reaching special interest due to their good specific properties, low cost and good manufacturing properties. However, their low hardness, wear and corrosion resistance limit their applications in certain sectors of industry. These drawbacks can be solved by applying hard ceramic coatings, such as nitrides or metal carbides. TiN is one of the most used coatings due to its high adhesion, hardness, low coefficient of friction and chemical stability. Physical vapor deposition by cathodic arc CAPVD, is a versatile technique, which uses low temperatures and high ionization energies, generating homogeneous coatings. To achieve coatings with high quality, a careful control of the manufacturing parameters is required, such as bias voltage, gas flow or intensity. This paper focuses on magnesium alloys, AM60, coated with TiN using physical vapor deposition cathodic arc technique (CAPVD at different intensity values (40A and 100A and surface preparation (grinding up to 4000 grit and polished to 3μm. It was included a final condition with an intermediate Al film. The samples were characterized by X-ray diffraction, roughness, optical microscopy and scanning electron.Las aleaciones de magnesio están alcanzando especial interés gracias a sus buenas propiedades específicas, bajo coste y buenas propiedades de moldeabilidad. No obstante su baja dureza, resistencia a desgaste y corrosión, limita sus aplicaciones en ciertos campos de la industria. Estos inconvenientes se pueden solucionar aplicando recubrimientos duros cerámicos, como nitruros o carburos metálicos. El TiN es uno de los más utilizados debido a su alta adherencia, dureza, bajo coeficiente de fricción y estabilidad química. La deposición física en fase vapor mediante arco catódico, ACPVD, es una técnica muy versátil, que emplea bajas temperaturas y altas energías de ionización, generando recubrimientos de bajo espesor, homogéneos y de calidad. Para alcanzar

  7. Obtención de sales biliares de magnesio mediante secado por aspersión

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    Jorge E Rodríguez Chanfrau

    Full Text Available Introducción: la industria farmacéutica cubana, desde la década del 60 del siglo XX, tiene entre sus líneas básicas de producción la obtención de ácidos biliares y sus derivados salinos a partir de la bilis bovina. Este proceso consiste en la saponificación de la bilis y la posterior precipitación del ácido cólico, el cual es transformado en la sal de magnesio mediante un proceso de reacción con hidróxido de magnesio. La materia prima activa obtenida es utilizada como ingrediente farmacéutico activo en la elaboración de tabletas para el tratamiento de desordenes gastrointestinales, así como en los síntomas producidos por un mal funcionamiento de la vesícula biliar. Objetivo: modificar el proceso de secado empleado en la actualidad mediante una variante de secado por aspersión. Métodos: se estableció un procedimiento para el secado; se realizaron los estudios a escala de banco. Se compararon los resultados con el proceso tradicional; se realizaron evaluaciones química, física y tecnológicas; entre ellas por microscopia electrónica y espectroscopia infrarroja. Resultados: al aplicar el procedimiento de secado por aspersión se obtuvo una materia prima similar en su composición a la obtenida en el procedimiento tradicional, pero con mejores características físico-químicas, lo que favoreció el proceso posterior de elaboración de la forma terminada. Conclusiones: partiendo de los resultados podemos afirmar que el proceso tecnológico de secado por aspersión desarrollado para la elaboración de las sales biliares de magnesio garantiza la calidad del producto final y mejora las condiciones de trabajo y condiciones ambientales.

  8. Tratamiento paliativo de los derrames pleurales malignos mediante abrasión química

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    Juan Antonio Castellanos González

    Full Text Available Introducción: el tratamiento del derrame pleural maligno constituye un reto para los cirujanos. Su erradicación permanente ha constituido un largo bregar, y para ello se ha utilizado un numeroso grupo de sustancias. Objetivo: mostrar los resultados del uso de diferentes sustancias químicas para conseguir la fusión de las pleuras en el derrame pleural maligno. Métodos: fueron estudiados 116 pacientes con derrames malignos de pleura tratados mediante toracocentesis y escleroterapia química en el Hospital Universitario "Comandante Manuel Fajardo" entre enero de 2001 y el 2013. El diagnóstico del 100 % de los pacientes fue clínico e imaginológico, con estudio del líquido pleural. Después de la toma de muestra se evacuó todo el contenido mediante un catéter colector y la instilación de la solución esclerosante. Como agentes irritantes se utilizó bleomicina, tetraciclina, amoxicilina y povidona yodada. Resultados: la causa más frecuente de derrame pleural resultó ser el cáncer de mama, seguido por el de pulmón y el ovario. En el hombre fue más frecuente el cáncer de pulmón y en la mujer el cáncer de mama. Predominó el sexo femenino y, específicamente, la sexta década de la vida. El 52,4 % de los pacientes necesitó dos sesiones terapéuticas y el 15,6 % no presentó recidiva después de la primera sesión. Conclusiones: los resultados fueron similares con todas las sustancias utilizadas. La intervención paliativa logró mejorar la calidad de vida al aliviar los síntomas de los pacientes y disminuir la hospitalización.

  9. Tratamiento del polvo de aluminio mediante disolución acuosa

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    López, F. A.

    2004-10-01

    Full Text Available Aluminium dust from aluminium remelting industry is a hazardous residue because of its high reactivity in the presence of water. In order to apply the new European Directive about landfill of waste, a study of its hydrolysis was carried out. The influence of temperature, time and pH on the hydrolysis of the aluminium dust was studied. The hydrolysed solids were characterized by XRD and AAS; in the aqueous solutions the pH and the ionic conductivity were determined. The evolved gases were analysed by mass spectrometry. The reactivity of the dust, before and after hydrolysis, was investigated by analysing the ammonia, hydrogen sulphide and metallic aluminium. By hydrolysis at 60 °C and 48 h a much lower reactive material was obtained which could be disposed with minimal environmental impact.

    El polvo de aluminio es un residuo generado en la metalurgia secundaria del aluminio y considerado peligroso como consecuencia de su elevada reactividad en presencia de humedad. Con objetivo de buscar un procedimiento de pretratamiento de dicho residuo, de acuerdo con la Directiva Europea sobre vertederos, se ha realizado el estudio del comportamiento del polvo de aluminio en medio acuoso. Para ello, se han analizado la influencia de la temperatura, el tiempo y el pH de reacción en su hidrólisis. Los sólidos hidrolizados se caracterizaron mediante EAA y DRX, mientras que en las soluciones acuosas resultantes se determinaron el pH y la conductividad iónica. Los gases liberados durante el proceso de hidrólisis se analizaron mediante espectrometría de masas. Asimismo, se ha determinado la reactividad del residuo antes y después de la hidrólisis, analizando amoniaco, sulfuro de hidrógeno y aluminio metálico. La hidrólisis, a 60 °C y después de 48 h, permite obtener material de muy baja reactividad que podría ser almacenado en vertedero.

  10. Estudio mediante afm de estructuras de silicalita para la separación de gases

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    Prádanos, P.

    2004-02-01

    Full Text Available In this work, we study films of silicalite crystals used in gas separation processes. These crystals were obtained by hydrothermal synthesis during different times and using different gel composition. They were deposited on an alumina support growing in two preferential directions. Finally, the material was placed in a stove at 480ºC during 8 h in order to remove the structurant agent with heating and cooling rates of 0.5 y 2 ºC/min respectively. The resulting surfaces were analysed by atomic force microscopy (AFM in tapping mode in order to notice the deposition of the silicalite crystals in the indicated directions, and also to distinguish the evolution of the nuclei growth. At the same time, the porous structure of silicalite has been determined, leading to results in good agreement with those obtained by other techniques.

    En este trabajo se han estudiado láminas de silicalita con aplicación en los procesos de separación de gases. Dichas láminas se han depositado mediante síntesis hidrotermal durante distintos tiempos y usando varias composiciones en el gel precursor. La deposición se hizo sobre un soporte de alúmina con crecimiento preferencial en dos direcciones. Finalmente las láminas se calcinaron a 753 K durante 8 h para eliminar el agente estructurante, usando velocidades de calentamiento y enfriamiento de 0.5 y 1 K/min respectivamente. Las superficies resultantes se han analizado mediante microscopía de fuerza atómica en modo de contacto-intermitente (tapping con el fin de ver si efectivamente se ha conseguido depositar cristales de silicalita en las direcciones indicadas y seguir la evolución de crecimiento de los núcleos. Por otro lado, se ha determinado la estructura porosa de la silicalita comprobando que los resultados concuerdan con los obtenidos por otras técnicas.

  11. Microestructura y propiedades de capas de tribaloy T-800 depositadas mediante plaqueado láser

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    Navas, C.

    2004-04-01

    Full Text Available The present work is based on obtaining Co based coatings (Tribaloy T-800 on plates of 18/8 stainless steel (AISI 304 by laser cladding technique. After the treatment, samples were characterized by optical and scanning electron microscopy with EDS analysis. The elemental composition of the coating was determined with a glow discharge lamp spectrometer (GDL. The study of the interface revealed a good adherence between the substrate and coating without substantial defects. For the laser cladded coatings, the microstructure was dendritic with a high degree of refinement and chemical homogeneity close to the original powder. The grain coarsening was observed in the overlapping zones due to the second heat treatment. Microhardness of the coatings reached 750 HV, a considerably higher value than the substrate hardness (200 HV. Also, the coating corrosion resistance in saline solutions had a great improvement.

    El presente trabajo se centra en la obtención de capas de aleación base Cobalto (Tribaloy T-800 sobre un sustrato de acero inoxidable 18/8 (AISI 304 mediante la técnica de plaqueado láser. Tras los tratamientos, se caracterizaron las probetas mediante microscopía óptica y electrónica de barrido con microanálisis (EDS. La composición elemental del recubrimiento se determinó en un espectrómetro de emisión óptica con fuente de excitación (GDL. El estudio de la interfase reveló una perfecta adherencia entre el recubrimiento y el material base, sin defectos apreciables. La microestructura de las capas depositadas es dendrítica con un alto grado de refinamiento y una homogeneidad química a lo largo del cordón y con valores muy próximos a los del polvo de partida. En las zonas de solape entre cordones, se observó un crecimiento del grano debido al segundo tratamiento térmico recibido. La microdureza de las capas alcanza los 750 HV, valor considerablemente superior al del sustrato (200 HV. Asimismo, se obtuvo una mejora

  12. Leptospira interrogans inducing apoptosis of macrophages through mitochondria-associated signaling pathway%问号钩端螺旋体经线粒体相关信号通路诱导巨噬细胞凋亡的研究

    Institute of Scientific and Technical Information of China (English)

    范兴丽; 董海艳; 严杰

    2009-01-01

    Objective To determine mitochondria-associated signaling pathway on cell apoptosis in Leptospira interrogans infected murine mononuclear-macrophages. Methods A cell apoptotic model of L. interrogan serogroup Icterohaemorrhagiae strain Lai inducing apoptosis of murine mononuclear-macrophage line (J774A. 1) was established. Pathological changes of mitochondria in the infected cells were observed under transmission electron microscope. Mitochondrial potential and reactive oxygen species(ROS) levels in the infected cells were detected using JC-1 staining method and DCFH-DA fluorescent probe, respectively,and caspase-8/-9 activities in the infected cells were measured using commercial kits. and the apoptosis block effects of caspase inhibitors were determined by flow cytometry. Apoptosis in the infected cells and ap-optosis-blocking effect by caspase inhibitors were detected by flow cytometry. Western blot assay was adopted to examine the levels of cytochrome C (CytC) , AIF, EndoG and Smae in the mitochondria and cytoplasm.The transposition of either AIF or EndoG from the cytoplasm into cell nucleus was determined by immunoflu-orescence staining test. Results L. interrogans strain Lai could induce J774A. 1 cell apoptoais. In the in-fected cells, visible mitochondrial injury, declined mitochondrial membrane potential and elevated ROS level were presented. The activity of caspase-8 but not of caspase-9 was significantly increased. The caspase in-hihitors could not completely block the cell apoptosis. Both the AIF and EndoG was released from the mito-chondria and subsequently transferred from the cytoplasm into the nucleus in the infected cells. However,elevation of CytC level and Smac release in cytoplasm of the infected cells could not be found. Conclusion L.interrogans can induce apoptosis in the infected mononuclear-macrophage via AIF and EndoG belonging to caspase-independent pathway.%目的 确定线粒体相关信号转导途径在问号钩端螺旋体(简称钩

  13. Detección de información oculta mediante potenciales relacionados con eventos

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    Odette Terol

    2014-01-01

    Full Text Available Existe un interés considerable en hallar métodos que nos ayuden a saber cuándo una persona miente y cuándo dice la verdad desde un punto de vista forense. Actualmente, una de las líneas de investigación se inclina hacia el uso de potenciales relacionados con eventos. Se pretende hacer una revisión de los artículos que estudian estos procedimientos mediante distintos métodos: propiedades, fiabilidad, validez y limitaciones. Los resultados indican tasas de acierto en la discriminación de culpables en un rango de 7 al 100 por ciento, y en la de inocentes de 31 a 100 por ciento. La gran variabilidad y la posibilidad de “falsear” las respuestas llevan a cuestionar la inexactitud utilizada en algunos círculos mediáticos respecto a las cualidades y finalidades de dicha prueba. Se concluye la necesidad de profundizar más la posibilidad de que esta prueba sea utilizada con fines forenses.

  14. Medida de similaridad entre imágenes de marcas de ganado mediante distribuciones de forma

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    Germán Sánchez Torres

    2014-12-01

    Full Text Available Este documento reporta los resultados de una investigación orientada hacia el diseño de un método de tratamiento de imágenes digitales para la automatización de los procesos de registro y control de marcas de ganado requeridas por las regulaciones del sector ganadero en Colombia. El método permite automatizar los procesos de búsqueda y de comparación necesarios para garantizar la unicidad de las marcas dentro de un sistema asistido por computadora. Se inicia con la generación de un histograma estimado de la geometría de la marca, lo que permite comparar y detectar similitudes entre las figuras previamente almacenadas, mediante una métrica de similitud basada en la distancia de Minkowski. Los resultados obtenidos indican que el método es adecuado para realizar un proceso de discriminación de dichas imágenes, reducir las ambigüedades y garantizar la unicidad de los registros. Los resultados obtenidos y un análisis de su aplicación son reportados.

  15. DESULFURACIÓN DE RELAVE MEDIANTE LA FLOTACIÓN DE SULFUROS DE HIERRO

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    Luis Valderrama Campusano

    2015-12-01

    Full Text Available En Chile la minería se desarrolla principalmente en  la concentración de cobre, molibdeno, oro y plata, mediante el proceso de flotación. Este genera grandes tonelajes de relaves que contienen diversas minerales, principalmente pirita. Dado que estos minerales se depositan en los tranques, estos pueden generar aguas ácidas; se propone como alternativa la desulfuración de los súlfuros por flotación. En una primera parte se estudió la flotabilidad de la pirita en un tubo Hallimond, utilizando como colector AP 404. Luego se realizaron pruebas de flotación a nivel de laboratorio en celda Denver D-12, utilizando pulpa de relave fresco de la etapa rougher de un circuito de flotación de cobre,  determinando el pH óptimo y dosificación de colector y espumantes. Pruebas de cinética de flotación permitieron determinar el tiempo óptimo de flotación tanto para la etapa rougher como  para la etapa scavenger. Se concluye que la flotación de los relaves sea una técnicamente factible su desulfuración,  obteniendo relaves que contienen  0,08% de pirita.

  16. Tratamiento del tortícolis congénito mediante control estimular y fisioterapia

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    MARÍA ASUNCIÓN VÁZQUEZ VILÀ

    2004-01-01

    Full Text Available Presentamos un caso clínico de tortícolis congénito y su tratamiento mediante control estimular y fisioterapia, por la alta frecuencia del tortícolis en la práctica clínica y por los buenos resultados que en general presenta el tratamiento conservador en estos casos, principalmente cuando éste se inicia de forma precoz. El caso presentado es de una niña con tortícolis congénito izquierdo, sin patologías asociadas, en tratamiento desde los 15 días hasta los nueve meses de edad, con una buena evolución. Mostramos los antecedentes, exploración y pruebas complementarias realizadas, el tratamiento, que se ha dividido en varios períodos (15 días- 2 meses; 2-4 meses; 4-9 meses, la orientación a los padres y la evolución de la niña. Como conclusión fundamental destacar la importancia del diagnóstico y tratamiento precoces, y la orientación a los padres para que estimulen al niño adecuadamente y colaboren en el tratamiento.

  17. Fenilcetonúria: estudo clínico e mediante biópsia cerebral

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    Aron J. Diament

    1967-03-01

    Full Text Available Foram relatados 6 casos de fenilcetonúria (oligofrenia fenilpirúvica, sendo os pacientes, irmãos dois a dois, pertencentes a três famílias distintas. Foram feitos estudos genealógico, clínico e laboratorial, incluindo a dosagem de fenilalanina sérica. O interesse maior foi o de mostrar a normalidade do estudo histopatológico realizado por meio de biópsias cerebrais em 4 casos, apesar de não ter sido feito estudo histoquímico, atualmente considerado como necessário. Esta normalidade das biópsias não teve qualquer relação com o nível mental, com as alterações eletrencefalográficas cu com atrofias cerebrais assinaladas em alguns casos mediante encefalografia gasosa. Todos os casos foram tratados com dieta especial (tipo Lofenalac complementada com alimentos de baixo teor em fenilalanina. Dosagens de fenilalanina sérica estão sendo feitas antes e depois do uso de tais dietas, sendo ainda muito cedo para qualquer conclusão.

  18. Prácticas educativas. Desarrollo de la lectura mediante estrategias integradoras

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    Maira Solé

    2005-01-01

    Full Text Available La lectura y la escritura son procesos que cada día ameritan nuevos cambios y transformaciones. La propuesta de un Proyecto Pedagógico Integrador, (Fraca 2003 desarrollado con éxito en algunas instituciones venezolanas, se perfila como una alternativa significativa para el desarrollo de estos elementos. La idea o núcleo central es la integración de las diferentes asignaturas curriculares y lograr una globalización partiendo de sus objetivos y contenidos programáticos. El eje pedagógico integrador le permite al docente, evidenciar con mayor prontitud los resultados mediante actividades prácticas de lectura y escritura. Así mismo combina elementos claves del aprendizaje ausbeliano: información previa, información nueva y construcción de la información definitiva o integrada. La puesta en ejecución de las estrategias integradoras, en esta ocasión por maestros en formación (UNEG, a diferentes niños de escuelas del Estado Bolívar (Venezuela, certificando cómo la lectura y la escritura pueden tener un espacio ideal y significativo en la instrucción actual. Solo se necesita la intención, creatividad, dinamismo e ingenio.

  19. Operación de un PLC Mediante un PDA Vía ZIGBEE

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    Juan Zuluaga-Botero

    2015-07-01

    Full Text Available En este artículo se muestra la parte preliminar de interconexión de un módulo ZigBee con un Asistente Digital Personal (PDA a través de la red del Sistema de telefonía Móvil Global (GSM, para aplicación futura de comunicación con un Controlador Lógico Programable (PLC. Esta aplicación permite realizar monitoreo y control del sistema, de manera remota y con dispositivos móviles siendo el alcance del proyecto de investigación. Para este proyecto inicialmente se realizan comunicaciones que permiten empalmar las tecnologías de las redes de telefonía móvil celular con la red ZigBee, haciendo una interfaz transparente para el usuario, presentando dichos resultados en este artículo. Para esto se trabaja con tramas de datos básicas a través de la red de telefonía móvil celular, mediante mensajería corta recibida por el puerto serial del Zigbee remoto, utilizando microcontroladores para el control de los módulos que conforman esta parte del sistema de comunicación, estableciendo la interfaz con el dispositivo móvil o Smartphone vía Zigbee.

  20. Análisis de una columna desbutanizadora mediante técnica de perfilaje gamma

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    Óscar Chaverri

    2007-05-01

    Full Text Available En este trabajo se reportan los resultados de un estudio, realizado mediante la técnica no destructiva de perfilaje por rayos gamma, de una columna desbutanizadora en una refinería de petróleo. Dicho estudio tuvo entre sus objetivos el de resaltar los beneficios que se derivan de la aplicación pacifica de la energía nuclear, en la determinación de problemas que afectan la eficiencia y seguridad de procesos industriales. En este caso particular, se inspeccionó parte de una torre para la destilación de petróleo, y se determinó la presencia de algunas pequeñas anomalías, aunque el funcionamiento de la columna era correcto y seguro. Cabe recalcar que,por tratarse de una técnica no destructiva y con la posibilidad de medir a través de la pared de la columna, el ensayo fue realizado sin necesidad de abrir la torre misma o de introducir personal técnico en su interior.

  1. Galactosemia: Diagnóstico precoz mediante estudio enzimático

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    Úrsula Carrillo Estrada

    2003-09-01

    Full Text Available Se presentan los resultados obtenidos del estudio realizado a un paciente masculino de 45 días de nacido, cuyo motivo de ingreso fue pérdida de peso, decaimiento, retraso psicomotor y crisis de hipoglucemia. Los síntomas comenzaron en el período neonatal y coincidieron con la introducción de la lactancia materna. En estudios realizados se constató en la orina la presencia de lactosa y galactosa. Se confirma el diagnóstico por estudio enzimático. La evolución clínica ha sido satisfactoria. El tratamiento dietético que excluía a los alimentos que contienen galactosa y lactosa fue de mucha importancia. Es el primer caso diagnosticado en Cuba mediante estudio enzimático.This paper presents the results of a study performed on a 45-day old male patient who was admitted to the hospital for weight loss, tiredness, psychomotor retardation and hypoglicemic crisis. The symptoms had begun in the neonatal period and had coincided with the introduction of breast feeding. The studies detected lactose and galactose in urine. The enzymatic study confirmed the diagnosis. The clinical recovery was satisfactory. The dietary treatment that excluded foods containing galactose and lactose was important and successful. He is the first case diagnosed on enzymatic study in Cuba.

  2. BIORREMEDIACIÓN DE UN SUELO CON DIESEL MEDIANTE EL USO DE MICROORGANISMOS AUTÓCTONOS

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    ARRIETA RAMÍREZ OLGA MARIA

    2012-07-01

    Full Text Available En este estudio, se aisló y caracterizó bioquímica y molecularmente un consorcio bacteriano capaz de degradar los diferentes hidrocarburos presentes en un combustible diesel,conformado por los siguientes géneros: Enterobacter sp, Bacillus sp, Staphylococcus aureus, Sanguibacter soli, Arthrobacter sp y Flavobacterium sp, a partir de un suelo contaminado con diesel a escala de laboratorio, y tratado mediante 2 tecnologías de biorremediación: atenuación natural y bioestimulación. Se definió como parámetro de control la concentración de Hidrocarburos Totales del Petróleo (HTP y para el cual, se obtuvo una reducción en la concentración en un periodo de 4 meses de 36,86% para atenuación natural y 50,99% para bioestimulación. La medición de la eficiencia de remoción de hidrocarburos se cuantificó por cromatografía de gases acoplada a masas (GC-MS.

  3. LA EXPERIENCIA DE UNA CLASE INTEGRADA MEDIANTE RESOLUCIÓN DE PROBLEMAS

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    Fernanda Ortiz Rivera

    2014-05-01

    Full Text Available Es una experiencia en la Institución educativa Tomas Cipriano de Mosquera con estudiantes del ciclo noveno en una clase de ciencias naturales sobre el sentido de la audición, teniendo en cuenta integrar la biología  y la física  mediante el tema del órgano de la audición con el tema del sonido, en el marco de un enfoque didáctico por resolución de problemas, en el cual la pregunta problema central que se generó a los estudiantes, producto de consultar sus intereses, fue: ¿por qué se produce la sordera? Esta pregunta se realizó al comienzo y al final de la clase, mostrando de esta manera al final buenos resultados de aprendizaje en los estudiantes, ya que fueron construyendo  nociones, ideas y conceptos necesarios sobre el sonido con sus propiedades y cualidades; y la audición con las funciones y partes del oído, para poder resolver la pregunta central gracias a una secuencia didáctica de clase que construimos cuidadosamente en el que incluía experimentos, lecturas, explicaciones, materiales didácticos, entre otros, siendo así una clase muy activa y participativa, ya que el estudiante siempre fue el que llevo el papel principal de la clase.

  4. Diseño mediante elementos finitos de componentes estructurales de un cuadricóptero para impresión 3D

    OpenAIRE

    PARDO APARISI, IVÁN

    2016-01-01

    El trabajo tiene como objetivo diseñar componentes estructurales, mediante el método de elementos finitos, que serán utilizados en un dron de cuatro rotores (cuadricóptero). Una característica particular de este proyecto es que los componentes estructurales a diseñar serán fabricados mediante impresión 3D. Pardo Aparisi, I. (2016). Diseño mediante elementos finitos de componentes estructurales de un cuadricóptero para impresión 3D. http://hdl.handle.net/10251/75994. TFGM

  5. Grafenos procesables en fase líquida con características optimizadas mediante el control microscópico de su estructura

    OpenAIRE

    Rozada Rodríguez, Rubén

    2014-01-01

    El objetivo principal de la tesis doctoral consiste en desarrollar estrategias que permitan mejorar las características de grafeno producido en fase líquida mediante la ruta del óxido de grafito, de modo que se pueda compatibilizar la relativa simplicidad y alto rendimiento de dicha ruta con la perfección estructural de las láminas de grafeno generadas mediante otros métodos. El desarrollo de dichas estrategias se ha apoyado en el estudio detallado de las láminas mediante diferentes técnicas ...

  6. Prokaryotic expression of tlyA gene of Leptospira interrogans strain and the significance of the ex-pressed protein in hemolysis and inflammation%问号钩端螺旋体 tlyA 基因原核表达及其表达产物溶血和致炎作用

    Institute of Scientific and Technical Information of China (English)

    王欢; 林旭瑷; 周永列; 邱莲女; 严杰

    2015-01-01

    目的:构建问号钩端螺旋体(简称钩体) tlyA基因原核表达系统,确定目的重组表达产物rTlyA溶解红细胞及诱导人THP-1和小鼠J774A.1巨噬细胞分泌致炎细胞因子的作用。方法采用PCR扩增问号钩体黄疸出血群赖型赖株tlyA基因,T-A克隆后测序。采用原核表达载体pET-42a及表达宿主菌E.coli BL21DE3构建tlyA基因原核表达系统。采用SDS-PAGE检测rTlyA表达情况, Ni-NTA亲和层析法提纯rTlyA。采用平板法和分光光度法测定rTlyA对绵羊红细胞的溶血活性。采用RT-PCR和Western blot分别检测问号钩体赖株感染THP-1和J774A.1细胞后tlyA基因mRNA水平变化及TlyA外分泌情况。采用ELISA检测rTlyA诱导THP-1和J774A.1细胞分泌致炎细胞因子IL-1β、IL-6和TNF-α的作用。结果所克隆的tlyA基因与GenBank中公布的相应基因核苷酸和氨基酸序列相似性分别为99.2%和99.8%。所构建的tlyA基因原核表达系统能有效表达rTlyA。10μg/ml rTlyA能显示较强的溶解绵羊红细胞活性。问号钩体赖株感染THP-1和J774A.1细胞1~8 h后,tlyA-mRNA水平显著升高(P<0.05)且出现TlyA外分泌。0.1、1和10μg/ml rTlyA均能使THP-1和J774A.1细胞分泌致炎细胞因子IL-1β、IL-6和TNF-α的水平显著升高(P<0.05)。结论问号钩体tlyA基因产物TlyA是溶血素并能诱导巨噬细胞分泌IL-1β、IL-6和TNF-α,可在钩体病炎症反应中发挥重要作用。%Objective To construct a prokaryotic expression system for tlyA gene of Leptospira in-terrogans ( L.interrogans) strain and to investigate the effects of the expressed rTlyA protein on the hemolysis of sheep erythrocytes and the secretion of pro-inflammatory cytokines by human THP-1 cells and murine J774A.1 macrophages.Methods The fragment of tlyA gene of L.inetrrogans serogroup Icterohaemorrhagiae serovar Lai strain Lai was amplified by PCR.The PCR product was sequenced after T

  7. DEMANDA TRIMESTRAL POR EMISIÓN MONETARIA: ESTIMACIÓN MEDIANTE TRES TÉCNICAS ESTADÍSTICAS

    Directory of Open Access Journals (Sweden)

    Carlos Torres G.

    2011-09-01

    Full Text Available Este documento presenta la estimación de la demanda trimestral por emisión monetaria mediante tres técnicas estadísticas, a saber: mínimos cuadrados ordinarios, corrección de errores y vectores autorregresivos. La estimación de la demanda se realizó para el periodo 1987–1997. En general las ecuaciones estimadas presentaron resultados satisfactorios en términos económicos y estadísticos. La capacidad de predicción se verificó con los bajos errores de pronóstico, inferiores al 3% para 1997. Se comprobó la estabilidad de la demanda de corto y largo plazo mediante los estadísticos Cusum y Cusum-Cuadrado, así como con la prueba de cointegración de Johansen.

  8. Extracción de ADN de Trypanosoma cruzi mediante tratamiento con bromuro de hexadecil-trimetil-amonio

    Directory of Open Access Journals (Sweden)

    Marcela Escalante

    1997-06-01

    Full Text Available En el presente trabajo se describe un método rápido, sencillo y eficaz para la obtención de ADN genómico de Trypanosoma cruzi, libre de impurezas y fácil de manipular. Dicho procedimiento se basa en la lisis del parásito con SDS y remoción de proteínas mediante la digestión con proteinasa K, seguida de la precipitación selectiva de carbohidratos y proteínas residuales con bromuro de hexadecil-trimetil-amonio (CTAB. Finalmente, el ADN se extrae con cloroformo: alcohol isoamílico y se recupera de la fase acuosa mediante precipitación con isopropanol.

  9. Producción de hidrógeno mediante fotocatálisis heterogénea

    OpenAIRE

    Rodríguez López, Cristina

    2015-01-01

    Programa de doctorado: Ingeniería ambiental y desalinización [ES]Se comienza con una revisión bibliográfica y una introducción a las técnicas y metodologías utilizadas en la parte experimental. A continuación se presentan los capítulos donde se exponen los resultados obtenidos en los experimentos realizados mediante fotocatálisis heterogénea.

  10. Superficie específica de una bentonita mediante la adsorción de azul de metileno

    Directory of Open Access Journals (Sweden)

    Jorge Alejo Pinzón Bello

    2010-07-01

    Full Text Available Se estudió la determinación de la superficie específica de una bentonita colombiana, procedente del Valle del Cauca, mediante la adsorción de azul de metileno, a 298 K. Este método se comparó con el de la adsorción de nitrógeno a 77 K.

  11. Método para el Modelado y Prueba de Diagramas de Actividades Mediante Redes de Petri

    Directory of Open Access Journals (Sweden)

    Lionel Baquero Hernández

    2015-11-01

    Full Text Available Las redes de Petri son ideales para describir y estudiar sistemas que procesan información y con características concurrentes, asíncronas, distribuidas, paralelas, no determinísticas y/o estocásticas. . Sin embargo, la teoría clásica de estas redes se enfrenta al desafío de adecuarse a las nuevas necesidades de los sistemas informáticos que los ingenieros diseñan en la actualidad. Un diagrama de actividades representa una secuencia de actividades. Podría aplicarse a cualquier propósito, pero se considera especialmente útil para visualizar los flujos de trabajo y los procesos del negocio, o casos de uso. El presente trabajo se ha desarrollado con el objetivo de diseñar un método para el modelado y prueba de diagramas de actividades mediante redes de Petri. El método propuesto brinda una serie de pasos necesarios para modelar un diagrama de actividades con redes de Petri y a partir de dicha red obtener una representación matemática de la misma. Esta representación matemática se utiliza para con ella realizar una serie de operaciones basadas en el marcaje de la red que permiten realizar pruebas al diagrama. Este método es capaz de identificar errores comunes que se cometen en el modelado de los diagramas de actividades, lo cual se demostró a través del método de experimentación.

  12. Selección de cartera mediante escenarios con dotación inicial indeterminada

    Directory of Open Access Journals (Sweden)

    Ventura Marco, M.

    1999-01-01

    Full Text Available Los modelos media-varianza de selección de cartera, y en general los basados en el binomio rentabilidad-riesgo, parten de un capital inicial cierto. La consideración de la aversión al riesgo en los mismos es indirecta y siempre referida al valor final de la cartera, así que por lo general lo que se determina en los mismos es la proporción de la inversión total o riqueza inicial que se destina a cada activo considerado. Sin embargo, en muchas situaciones reales la aversión al riesgo debe considerarse ya en el momento de invertir, por lo que la determinación de la cuantía a invertir, coste o dotación inicial forma parte del problema, considerando que será mayor, dentro de unas cotas, si existe la probabilidad de obtener mayor rentabilidad aún soportando mayor riesgo. El problema se presenta mediante un modelo simple, pero general en su planteamiento, en el que además de la composición de la cartera queda determinada la cantidad a invertir. La idea es que el inversor establece dos capitales, uno como mínimo y otro como máximo, que está dispuesto a invertir y se utiliza la optimización por escenarios para decidir qué cantidad, entre las dos anteriores, le conviene invertir según sus expectativas.

  13. Cleaning of contaminated soils with hydrocarbons by biocell; Saneamiento de suelos contaminados con hidrocarburos mediante biopilas

    Energy Technology Data Exchange (ETDEWEB)

    Iturbe-Arguelles, R.; Flores-Torres, C; Chavez-Lopez, C.; Roldan-Martin, A [Universidad Nacional Autonoma de Mexico, Mexico, D.F. (Mexico)

    2002-03-01

    In 1990 the Instituto de Ingenieria de la UNAM, initiated an evaluation through the soil and groundwater sampling and a risk health assessment in a Mexican refinery. An extended area was found contaminated with hydrocarbons. This area requires a soil remediation, taking into account that some zones present more than 30 000 mg/kg of Total Petroleum Hydrocarbons (TPH). Biopile system was recommended as the best remediation method to diminish TPG and some poliaromathic hydrocarbons (PAH). Therefore an experimental biopile of 30 m3 was constructed with contaminated soil. After 22 weeks, results show more than 80 % of TPH and PAH remotion. [Spanish] El grupo de saneamiento de suelos y acuiferos del Instituto de Ingenieria de la UNAM, inicio en 1999 la evaluacion de la contaminacion del subsuelo de una refineria en una zona costera del pais, mediante el muestreo de 425 puntos a 1.5 m de profundidad y con el analisis de los siguientes parametros: hidrocarburos totales del petroleo (HTP), hidrocarburo poliaromaticos (HAP), diesel, gasolina, metilterbutileter (MTBE) y los metales hierro, vanadio, zinc, cadmio, cromo y plomo. Asimismo, se lleva a cabo una evaluacion de riesgo a la salud a fin de determinar los niveles de limpieza de las areas contaminadas. Una vez realizado el estudio se propuso probar a nivel piloto dos tecnicas de saneamiento para las areas contaminadas con valores superiores a 30 000 mg/Kg de http, o bien, para las zonas en donde la evaluacion de riesgo a la salud indica la existencia de riesgo para uno o mas compuestos. Las tecnicas propuesta son biopilas y lavado de suelos con surfactantes. En este trabajo se presenta la prueba piloto con biopilas, de la cual se obtuvo una eficiencia de remocion de http del 80 porciento con cinco meses de operacion. Se muestra las partes de una biopila y se dan los resultados de la biopila experimental en la refineria Francisco I. Madero.

  14. Estudio del crecimiento de Prioria copaifera (Caesalpinaceae mediante técnicas dendrocronológicas

    Directory of Open Access Journals (Sweden)

    Jorge Andrés Giraldo Jiménez

    2011-12-01

    Full Text Available El cativo (Prioria copaifera G. forma bosques dominados por la especie llamados cativales. Durante más de 70 años el cativo ha sido la base principal de la industria maderera en el Darién colombiano, siendo, por su alta productividad y dominancia uno de los bosques tropicales más fáciles de ordenar sosteniblemente. El propósito de esta investigación es modelar el crecimiento del diámetro y el volumen del cativo en función de la edad así como las tasas instantáneas, medias y relativas, empleando anillos de crecimiento. La anualidad de los anillos se demostró usando el efecto de las bombas nucleares y mediante cofechado. Con base en los anillos de crecimiento radiales se modelo el crecimiento del diámetro y del volumen. Esta especie alcanza 40cm de diámetro en 90 años y presenta crecimiento medio de 0.31cm/año. El lapso vital de la especie es de aproximadamente 614 años. El incremento corriente anual máximo del volumen a los 90 años es 0.033m3/año, e iguala al incremento medio a los 145 años con 0.018m3/año. La tasa media de crecimiento absoluta es 0.021m3/año. Los resultados aquí obtenidos sirven como base técnica para el manejo sostenible de los cativales.

  15. PoV-GAME: PUNTOS DE VISTA MEDIANTE JUEGOS PoV-GAME: VIEWPOINTS THROUGH GAMES

    Directory of Open Access Journals (Sweden)

    Carlos Zapata-Jaramillo

    2012-06-01

    Full Text Available El desarrollo de proyectos de software considera importante la consistencia entre la información entregada por todos los interesados de una aplicación. En otras palabras, se procura llegar a acuerdos entre los diferentes puntos de vista de cada actor y llevarlos a cabo durante todo el proyecto. Con los métodos de enseñanza tradicionales se procura formar habilidades en este campo, pero no se afianzan mediante la práctica que, generalmente, se emplea para el aprendizaje en este campo. Por ello, en este artículo se propone ''PoV-GAME'', una nueva forma de ''jugar aprendiendo'', la cual busca fortalecer algunos conceptos básicos en la ingeniería de software como la consistencia en la información que se maneja a lo largo de un proyecto y afianzar la importancia de los puntos de vista en el desarrollo de un producto de software. Posteriormente, se analizan los resultados obtenidos luego de realizar el juego en diferentes grupos de estudiantes.Information for software applications is delivered by the stakeholders. Software development projects need consistency as an important aspect of such information. In other words, agreement among the different viewpoints must be reached in order to be implemented in the project. Well-known teaching methods try to create capabilities in this field, but they fail in reinforcing them in practice, which is commonly used for teaching in this field. Therefore, in this paper we propose ''PoV GAME'' a new way of ''play-to-learn''. We aim the reinforcement of some basic concepts about software engineering such as consistency in the information handled throughout the project, and the importance of viewpoints in the software product development. Subsequently, we analyze the results obtained after holding the game in several groups of students.

  16. Diagnóstico de Fallos en Sistemas Híbridos Mediante Anidamiento Latente de Fallos

    Directory of Open Access Journals (Sweden)

    Orestes Llanes Santiago

    2010-09-01

    Full Text Available Normal 0 21 false false false ES-TRAD X-NONE X-NONE MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} Este artículo presenta un nuevo enfoque para la detección y diagnóstico de fallos (SDDF en sistemas híbridos denominado de Anidamiento Latente de Fallos. La misma está basada en el uso de Redes de Petri Coloreadas (RdPCs que aportan su capacidad intrínseca de síntesis y de implementación en modelos realizables. Esta técnica se contrapone al clásico fenómeno de explosión combinacional que se produce mediante la utilización de metodologías basadas en Máquinas de Estados Finitos (MEFs.

  17. Cuantificación de inmunoglobulinas mediante el espectrofotómetro Shimadzu 160-A

    Directory of Open Access Journals (Sweden)

    Martha Leonor Paradoa Pérez

    1997-08-01

    Full Text Available Se realizó la adaptación al espectrofotómetro Shimadzu 160-A para cuantificar inmunoglobulinas (IgG, IgA e IgM por el método turbidimétrico Fixed time para sistemas Hitachi. El control de calidad se realizó con productos de referencia nacionales e internacionales para precisión y exactitud. Los resultados de las medias (IgG = 12,31, IgA = 2,13 e IgM = 1,38 y desviaciones estándar (IgG = 0,3885, IgA = 0,155 e IgM = 0,129 muestran una dispersión pequeña, y aseguran la validez de nuestro trabajo. Con este estudio se abre la posibilidad de realizar dicha técnica mediante un método rápido y eficaz en un equipo mucho menos costoso que el Hitachi y ampliamente difundido en Cuba.The fixed time turbidimetric procedure for Hitachi system was adapted to the spectrophotometer Shimadzu 160-A. The quality control was carried out with products of national and international reference to check precision and accuracy. The results of the means (IgG=12.31, IgA=2.13 and IgM = 1.38 and standard deviations (IgG=0.3885, IgA=0.155 and IgM=0.129 show a little dispersion and ensure the validity of our method. With this study, it is possible to put into practice this technique by a fast and efficient method in an equipment that is widely used in Cuba and much cheaper than the Hitachi.

  18. Veneno de abeja: Vigilancia tecnológica mediante el estudio de las citas de patentes

    Directory of Open Access Journals (Sweden)

    Beatriz García Delgado

    2008-01-01

    Full Text Available Los productos apícolas, particularmente el veneno de abeja, se encuentran entre los productos que poseen importantes propiedades farmacológicas y que en la actualmente su uso se diversifica, de aquí, la necesidad de realizar de forma sistemática estudios de vigilancia tecnológica relacionadas con este tema. En este trabajo, se realiza un estudio a partir de las 28 patentes que reivindican el "veneno de abeja" (concedidas por la Oficina de Patentes y Marcas de Estados Unidos y las citas correspondientes a estas invenciones. Esta información fue procesada con un sistema automatizado desarrollado para el procesamiento de patentes y sus citaciones, el cual genera un fichero que contiene la información procesada y que posibilita su posterior visualización en forma de red, mediante la utilización del sistema NetDraw, lo que permite, de forma eficaz y con inmediatez, identificar aquellas agrupaciones de interés que están relacionadas directamente con el veneno de abeja. Se determinó que existe una tendencia positiva en el desarrollo tecnológico relacionado con equipos y métodos para la recolección del veneno de abeja, y se comprobó que se continúan patentando procesos y productos relacionados fundamentalmente con el aislamiento, purificación y preparación de proteínas, anticuerpos y juegos de reactivos de diagnóstico que se emplean en la identificación y tratamiento de enfermedades inmunológicas.

  19. Combate del moho gris (Botrytis cinerea de la fresa mediante Gliocladium roseum

    Directory of Open Access Journals (Sweden)

    N\\u00E9stor Chaves

    2004-01-01

    Full Text Available En la zona de Poasito de Alajuela, se evaluó la acción del antagonista Gliocladium roseum, en forma individual y en conjunto con los fungicidas empleados en la finca, para el combate de Botrytis cinerea en fresa; comparándose los resultados contra los obtenidos con el manejo comercial. Se empleó un diseño de bloques completos al azar con 4 repeticiones y se hicieron aplicaciones semanales del antagonista (a una concentración ³ 107 conidios ml-1 durante un período aproximado de 4 meses (julio-octubre del 2000. Se evaluó la incidencia de moho gris en condiciones de campo y poscosecha, así como el efecto de los fungicidas aplicados sobre la germinación de los conidios del antagonista, mediante una prueba in vitro. Se obtuvo un combate más efectivo de la enfermedad en condiciones de campo al emplear el biocontrolador sólo o en conjunto con los fungicidas, con respecto al manejo comercial que se hace de la misma. En poscosecha, el desempeño del antagonista fue estadísticamente igual al del combate químico. Estos resultados muestran que los fungicidas aplicados no afectan considerablemente al antagonista, lo que se corroboró con la prueba in vitro. Al emplear G. roseum para el combate de B. cinerea no sólo se logra combatir efectivamente a este, sino también el resto de los patógenos (Colletotrichum, Phytophthora, Rhizoctonia, Rhizopus,Alternaria, Fusarium, Verticillium y Penicillium, ya que el porcentaje de frutas sanas es mayor al integrar la acción del antagonista al manejo de enfermedades de la finca. Sin embargo, estas diferencias no son estadísticamente significativas. Por lo anterior se concluye que G. roseum constituye una posible alternativa de manejo integrado del moho gris en fresa.

  20. Caracterización microestructural de aleaciones base cobre obtenidas mediante molienda reactiva

    Directory of Open Access Journals (Sweden)

    Palma, R.

    2010-06-01

    Full Text Available The micro and nanostructure of Cu-Al, Cu-V and Cu-Ti alloys produced by reactive milling were analyzed using X-ray diffraction (XRD and transmission electron microscopy (TEM. Samples with different milling times (t= 0, 10, 20 and 30 h were considered. The grain size, dislocation density and residual microstrain were evaluated form the XRD data using the Williamson-Hall and Klug-Alexander methods. The evolution of texture as a function of milling time was also studied using XRD. It was found, using TEM, that the grain size and dispersoid size were nanometric in all three alloys considered.

    Se analizó la micro y nano estructura de aleaciones Cu-Al, Cu-V y Cu-Ti obtenidas por molienda reactiva, mediante difracción de rayos X (XRD y microscopía electrónica de transmisión (TEM. Se consideraron muestras con distintos tiempos de molienda (t= 0, 10, 20 y 30 h. A partir de los datos XRD, usando los métodos de Williamson- Hall y Klug-Alexander, se evaluaron el tamaño de grano, la densidad de dislocaciones y la microdeformación residual; también se estudió la evolución de la textura de la matriz de cobre en función del tiempo de molienda. En los polvos molidos durante 30 h, de las tres aleaciones consideradas, se encontró, por TEM, que los tamaños de grano y de los dispersoides desarrollados son nanométricos.

  1. Instalación eléctrica de una vivienda unifamiliar aislada mediante suministro de energías renovables

    OpenAIRE

    LOZANO VALLADOLID, FERNANDO

    2015-01-01

    [ES] Instalación eléctrica con grado de electrificación elevada de una vivienda unifamiliar aislada mediante suministro de energías renovables (solar, eólica, geotérmicas). Lozano Valladolid, F. (2015). Instalación eléctrica de una vivienda unifamiliar aislada mediante suministro de energías renovables. http://hdl.handle.net/10251/58751. TFGM

  2. Instalación eléctrica de una vivienda unifamiliar aislada mediante suministro de energías renovables

    OpenAIRE

    LOZANO VALLADOLID, FERNANDO

    2015-01-01

    [ES] Instalación eléctrica con grado de electrificación elevada de una vivienda unifamiliar aislada mediante suministro de energías renovables (solar, eólica, geotérmicas). Lozano Valladolid, F. (2015). Instalación eléctrica de una vivienda unifamiliar aislada mediante suministro de energías renovables. http://hdl.handle.net/10251/58751. TFGM

  3. Efecto del argon en películas CNxHy depositadas mediante ECR-CVD

    Directory of Open Access Journals (Sweden)

    Albella, J. M.

    2004-04-01

    Full Text Available Carbon nitride films have been deposited by ECR-CVD, from Ar/CH4/N2 gas mixtures with different methane concentrations. Infrared Spectroscopy (IRS and Elastic Recoil Detection Analysis (ERDA have been used for films characterisation and Optical Emission Spectroscopy (OES for plasma analysis. Argon concentration in the gas mixture controls the growth rate as well as the composition of the film. In the proposed model, argon plays a key role in the activation of methane molecules. Also, during the growth of the film, two processes may be considered: i Film formation and ii Etching of the growing surface. Changing the gas mixture composition affects both processes, which results in films with different composition and structure as well as different deposition rates.Se ha estudiado el efecto del argon durante el proceso de CVD asistido por un plasma ECR para la síntesis de películas de nitruro de carbono (CNxHy a partir de mezclas gaseosas Ar/CH4/N2 con diferente contenido de metano. Las películas depositadas han sido analizadas mediante espectroscopía infrarroja (IRS y ERDA (Elastic Recoil Detection Analysis, y el análisis del plasma ha sido realizado utilizando la técnica de espectroscopía de emisión óptica (OES. La velocidad de deposición y la composición de las películas depositadas se encuentran determinadas por la concentración de argon en la mezcla gaseosa. Se propone un modelo, según el cual el argon juega un papel fundamental como activador de las moléculas de metano. El modelo propuesto incluye dos procesos simultáneos durante el crecimiento de las capas : i formación de la capa y ii ataque de la superficie de crecimiento. Según la composición de la mezcla gaseosa se favorece uno u otro proceso, lo que conduce a velocidades de deposición diferentes así como a depósitos con diferente composición y estructura atómica.

  4. Obtención de Si3N4 mediante SHS

    Directory of Open Access Journals (Sweden)

    Rodríguez, M. A.

    2003-04-01

    parámetros que definen la propia reacción. Con la información obtenida se propone el mecanismo predominante de la síntesis del Nitruro de Silicio mediante SHS.

  5. Terapia de exposición mediante Realidad Virtual en los trastornos alimentarios: presente y futuro

    Directory of Open Access Journals (Sweden)

    Ferran Vilalta Abella

    2015-01-01

    Full Text Available El objetivo de este trabajo fue revisar el uso de la realidad virtual (RV como técnica de exposición para el tratamiento de los trastornos de la conducta alimentaria (TCA. Para ello se analizó la literatura existente con el fin de responder a dos cuestiones: primero, ¿existe evidencia sobre la capacidad de los entornos y estímulos virtuales para provocar respuestas de ansiedad y craving en pacientes con TCA?; y, segundo, ¿la aplicación de técnicas de exposición mediante RV permite la disminución de esas respuestas? Se seleccionaron 13 estudios teniendo en cuenta los siguientes criterios de inclusión: muestras de pacientes con TCA, población subclínica y/o población sin TCA; exposición a entornos y estí- mulos de RV; evaluación de la ansiedad y/o craving; estudios empíricos y meta-análisis. Los resultados muestran evidencia sobre la capacidad de la RV para provocar respuestas de ansiedad en los pacientes con TCA y, por tanto, su eficacia potencial para la implementación de la terapia de exposición en el tratamiento de los trastornos alimentarios. En cambio, la evidencia disponible sobre la capacidad de la RV para generar respuestas de craving es limitada. Se detectan dos líneas de investigación emergentes. En primer lugar, el uso de la RV para la exposición con prevención de respuesta de la conducta de atracón en pacientes con bulimia y trastorno de atracón. En segundo lugar, el uso de esta tecnología para la exposición a la comida en pacientes con anorexia que muestran elevados niveles de ansiedad y evitación ante determinados alimentos y situaciones.

  6. Tribología de nuevas capas autolubricantes producidas mediante PVD

    Directory of Open Access Journals (Sweden)

    Oñate, J. I.

    2005-12-01

    Full Text Available MoS2 coatings, deposited by PVD show a low friction under vacuum conditions, but degrade easily under atmospheric conditions. This work describes the development of a new type of MoSx, coating, modified with WC. These coatings presented a very low and stable friction coefficient lower than 0.04 when tested under vacuum at 0.75 GPa in a ball on disc tribometer. Durability was higher than 1 million wear cycles, showing a wear rate of 1.3 10-16 m3/Nm. When tested under atmospheric conditions and up to 70 % RH, friction increased to 0.15 and durability reached a lower value of up to 3 x 105 cycles. Surface analyses in these coatings showed S/Mo ratios higher than 1.2 and with oxygen levels lower than 2 %, demonstrating the lubricant character of the coating.

    Las capas de MoS2 depositadas mediante PVD tienen baja fricción en vacío pero se degradan con facilidad al aire, especialmente en presencia de humedad. Este trabajo describe el desarrollo de nuevas capas de MoSx modificadas con WC. En ensayos de "bola sobre disco" a 0,75 GPa, las capas presentaron una fricción muy baja y estable (< 0,04 en vacío. Se obtuvo una durabilidad superior a 1 millón de ciclos, mostrando un desgaste de 1,3 x 10-16 m3/Nm, mejorado respecto a otras capas de MoSx. El coeficiente de fricción aumentó a 0,15 y la durabilidad disminuyó hasta 1 a 3 x 105 ciclos en aire hasta 70 % HR. El análisis superficial mostró ratios de S/Mo superiores a 1,2, con menos del 2 % de oxígeno, demostrando carácter lubricante.

  7. EDUCACIÓN MEDIANTE ENCICLOPEDIAS VISUALES TEMÁTICAS: INTYPEDIA UN CASO DE ÉXITO

    Directory of Open Access Journals (Sweden)

    ALFONSO MUÑOZ MUÑOZ

    2013-01-01

    Full Text Available La presente investigación recoge una experiencia de innovación educativa que demuestra la utilidad de las enciclopedias visuales temáticas para la formación académica a través de Internet. Los objetivos de esta experiencia recaen en verificar si es posible crear enciclopedias visuales temáticas que mediante recursos audiovisuales permitan el aprendizaje de aspectos complejos de una manera asequible. Con el interés de obtener datos que permitan comprobar la idoneidad de este tipo de recursos formativos, se construye una enciclopedia visual temática centrada en aspectos de la seguridad de la información y protección de las comunicaciones digitales. Para el diseño de esta enciclopedia se considera una metodología de diseño basada en la definición de una temática de interés, tipo de aprendizaje, formato y calidad de las unidades temáticas, difusión y coste, etc. Esta enciclopedia visual, el proyecto Intypedia, novedoso a escala mundial, está compuesta por lecciones en vídeo de unos 12 minutos y material docente complementario. El lenguaje utilizado permite el correcto seguimiento de las lecciones tanto a expertos en seguridad como internautas en general. Por tanto, la calidad y claridad de estos contenidos se convierten en un valor añadido del proyecto. Los resultados derivados reflejan la utilidad de Intypedia. En 2 años de vida la enciclopedia recibe miles de visitas al mes y se ha convertido en una enciclopedia de referencia en Iberoamérica y en las redes sociales en las que está presente. Algunos de los resultados más importantes son: 232.786 reproducciones únicas de las lecciones, siendo los consumidores principalmente internautas, profesionales y técnicos de España, Iberoamérica y EE.UU.; más de 176.000 documentos descargados, en torno a los 2.100 seguidores uniendo las redes sociales y una visibilidad en Google con más de 40.000 entradas que enlazan al proyecto.

  8. Standardizzazione isogravitá di un case-mix ospedaliero mediante Charlson index

    Directory of Open Access Journals (Sweden)

    G. Messina

    2003-05-01

    Full Text Available

    Introduzione: la comorbidità è un importante fattore confondente negli studi epidemiologici valutativi dell’assistenza ospedaliera. Diversi strumenti di risk adjustment misurano la complessità della malattia, consentendo di correlarla al consumo di risorse assistenziali, agli esiti, nonchè di confrontare studi eseguiti in tempi e realtà diversi.

    Obiettivi: - Standardizzare per complessità casistica la mortalità dei pazienti assistiti da un grande ospedale; - Identificare le variabili in grado di migliorare la capacità predittiva di mortalità intraospedaliera (IM. Materiali e

    Metodi: sono state analizzate 40.801 schede di dimissione prodotte dal Policlinico Senese nel 2001. Sono stati studiati i tassi di IM specifici per Charlson Index Score (CSI: quest’ultimo considera 19 categorie di patologia e si basa sull’ ICD-IX-CM. Le variabili studiate mediante analisi bivariate e regressione logistica, sono state: CSI (codificato in 5 livelli 0, 1, 2, 3, 4, lunghezza del ricovero (LR, sesso ed età. Risultati: CSI è risultato associato con IM (p‹0.001. Sesso, età e LR sono risultati associati sia con CSI (p‹0.001 che IM (p‹0.001. Confrontando i 4 livelli di CSI con quello di riferimento (0, le seguenti Odds Ratio (OR di IM sono state trovate: Livello 1 verso livello 0 OR: 6.79 (p‹0.001, Livello 2 verso livello 0 OR: 15.8 (p‹0.001, Livello 3 verso livello 0 OR: 9.36 (p‹0.001, Livello 4 verso livello 0 OR: 7.4 (p‹0.001. La variabile sesso non è risultata aver un effetto confondente tra CSI e IM al contrario delle variabili LR ed età.

    Conclusioni: il CSI aiuta a valutare (predire il rischio di mortalità intraospedaliera, sebbene in modo non lineare. Abbiamo sempre rilevato valori più alti di mortalità confrontando i livelli 1, 2, 3, e 4 con quello di riferimento (0. In particolare il valore più alto lo ha raggiunto il secondo livello

  9. RECUBRIMIENTOS DE CARBUROS DE Nb-V-Cr DEPOSITADOS MEDIANTE EL PROCESO DE DIFUSIÓN TERMO-REACTIVA (TRD

    Directory of Open Access Journals (Sweden)

    Anyerson Cuervo

    2015-01-01

    Full Text Available En este trabajo se presentan los resultados del proceso de depósito de recubrimientos de carburos de niobio-vanadio-cromo sobre acero AISI/SAE 1045 y AISI/SAE H13, utilizando la técnica de deposición por difusión termorreactiva (TRD. Los recubrimientos fueron obtenidos usando un baño de sales compuesto por bórax fundido, ferro-niobio, ferro-vanadio, ferro-cromo y aluminio, variando el contenido de cromo (8 y 32 % en peso, a una temperatura de 1.020°C durante 4 horas. Mediante la técnica de Difracción de Rayos X se evidenció la presencia de fases binarias de VC, NbC, CrC. Las propiedades químicas superficiales y morfológicas de los recubrimientos se estudiaron mediante la técnica de microscopia electrónica de barrido con acople de una sonda de energía dispersiva (MEB-EDS. La dureza de los recubrimientos se determinó utilizando un microindentador LECO M-400-G2 con una carga máxima de 300 g. El comportamiento electroquímico fue estudiado a través de curvas de polarización potencio- dinámicas para determinar la densidad de corriente de corrosión. El comportamiento tribológico se analizó mediante el ensayo “ Ball-on-disc ”, encontrando un coeficiente de fricción menor en los materiales recubiertos con respecto al sustrato sin recubrir.

  10. Sulla stima di macro, micro e nano-durezza di materiali metallici mediante analisi elasto-plastiche agli elementi finiti

    Directory of Open Access Journals (Sweden)

    Luca Susmel

    2012-01-01

    Full Text Available Il lavoro sintetizzato nel presente articolo si pone come obiettivo primario quello di investigare la possibilità di stimare, mediante un approccio elasto-plastico agli elementi finiti, la durezza dei materiali metallici convenzionali, e questo sia a livello macroscopico, che a livello microscopico, che, infine, a livello nanoscopico. Per verificare validità e accuratezza della metodologia FEM sviluppata, sono state condotte una serie di analisi sperimentali su tre materiali metallici aventi caratteristiche metallurgiche estremamente diverse: una lega d’alluminio (Al 7075-T6, un acciaio a basso tenore di carbonio (BS970-En3B e, infine, un acciaio austenitico (AISI 316L. L’indentazione Vickers è stata simulata con analisi elasto–plastiche agli elementi finiti considerando carichi di prova nell’intervallo tra 490 N e 490 µN e calibrando le simulazioni numeriche mediante curve monotone tensione–deformazione ottenute da prove di trazione eseguite utilizzando provini sia di dimensione convenzionale che aventi larghezza della zona calibrata dell’ordine dei 100 µm.La sistematica comparazione tra risultati sperimentali e simulazioni numeriche ha posto in evidenza come l’aumentare del valore della durezza misurata al diminuire della dimensione dell’impronta possa essere imputata al ruolo giocato dalla reale morfologia del materiale, ruolo che diventa predominante sulla plasticità convenzionale quando le dimensioni della superficie indentata diventano comparabili con le dimensioni medie della grana cristallina delle leghe esaminate. Tali fenomeni, pertanto, non hanno consentito di estendere l’utilizzo della meccanica del continuo fino ad un livello nanoscopico per determinare correttamente i valori della durezza. Alla luce di questi risultati è stata, però, proposta una semplice metodologia di correzione delle stime eseguite mediante gli elementi finiti che si è dimostrata un valido strumento da utilizzarsi in situazioni di

  11. Modificación de las propiedades superficiales de aceros inoxidables Dúplex mediante recubrimientos por láser

    Directory of Open Access Journals (Sweden)

    Amigo, V.

    2004-12-01

    Full Text Available Laser cladding is one of the most promissing techniques to restore damaged surfaces and achieve properties similar to those of the base metal. In this work, duplex stainless steels have been cladded by a nickel alloy under different processing conditions. The influence of the beam speed and defocusing variables has been evaluated in the microstructure both of the cladding and heat affected zone, HAZ. These results have been correlated to mechanical properties by means of microhardness measurements from cladding area to base metal through the interface. This technique has shown to be very appropriate to obtain controlled mechanical properties as they are determined by the solidification microstructure, originated by the transfer of mass and heat in the system.

    La posibilidad de restaurar superficies y, con ello, las propiedades de las mismas mediante el recubrimiento por láser de polvos constituye uno de los mayores intereses en las investigaciones actuales. En este trabajo se ha obtenido un recubrimiento de una aleación base níquel, mediante el tratamiento láser de polvos elementales para diferentes condiciones de procesado. Se ha evaluado la influencia de las variables de proceso, velocidad del láser y desenfoque del haz láser en la microestructura de los recubrimientos y con ello en las propiedades finales de los mismos, así como en la transición de estas a través de la interfase y la zona afectada por el calor, ZAC, evaluada mediante perfiles de microdureza. La obtención de recubrimientos a partir de polvos resulta muy adecuada, en este caso, al obtener unas propiedades mecánicas que están determinadas por la microestructura de solidificación, originada por la transferencia de masa y calor en el sistema.

  12. Síntesis de nitruro de titanio mediante láser y energía solar concentrada

    Directory of Open Access Journals (Sweden)

    García, I.

    1998-04-01

    Full Text Available The possibility of the employment of solar energy concentrated by Fresnel lens is investigated in order to synthesize materials by gas-solid reaction. These first results are compared by two similar techniques as high power laser and xenon are lamp. The TiN coatings obtained with xenon are lamp and Fresnel lens are homogenous, without pores or defects, with a uniform thickness of about 6 μm for treatments of 2 min. The good quality of the TiN coating for all the testing conditions was confirmed by the x-ray diffraction measurements.

    Se presenta la utilización de la energía solar concentrada mediante lentes de Fresnel para la síntesis de materiales por reacción gas-sólido. Estos primeros resultados sobre nitruración superficial de titanio y aleaciones de titanio se comparan con los obtenidos con técnicas similares como el láser de alta potencia y la lámpara de descarga de xenón. Las capas de nitruro de titanio obtenidas mediante energía solar concentrada por lentes de Fresnel y lámpara de xenón son homogéneas, sin grietas ni defectos, y con un espesor uniforme de 6 μm en tiempos de sólo 2 min. La buena calidad de estas capas se confirma mediante difracción de rayos X.

  13. Desarrollo de un sistema alternativo para la implementación de telemedicina mediante internet inalámbrico

    OpenAIRE

    2009-01-01

    En la actualidad las telecomunicaciones desempeñan un papel muy importante en el área médica; los avances tecnológicos y la Internet resultan a menudo herramientas imprescindibles en este campo. Debido a que la accesibilidad a este medio y su uso van en aumento, esta propuesta consiste en desarrollar un sistema que mantenga en constante comunicación al médicoy paciente, mediante la Internet. Al mismo tiempo se brinda un sistema de información médico que contiene datos útiles tanto para...

  14. Optimización de un cuestionario mediante un método Delphi y una prueba piloto

    OpenAIRE

    David Jiménez Hernández; María Tornel Abellán; Juan José González Ortiz

    2015-01-01

    Por medio del presente artículo describimos un Método Delphi y una prueba piloto para la mejora de un cuestionario sobre métodos activos de enseñanza. En el primer instrumento se utilizan las opinión de ocho expertos de diferentes temáticas que a través de cinco rondas fueron presentando propuestas de mejora para el cuestionario. Mediante la prueba piloto, además de comprobar el comportamiento del cuestionario en una parte proporcional de la muestra, también ayudó a la resolución de algunas c...

  15. Propuesta de mejora de habilidades sociales en niños con discapacidad intelectual mediante la terapia asistida por animales

    OpenAIRE

    Bermejo Lorenzo, Julia

    2015-01-01

    Este Trabajo de Fin de Grado se centra en la aplicación práctica de un programa de mejora de la Educación Socioemocional, utilizando como herramienta innovadora la Terapia Asistida con Animales de Compañía, aplicándose a un caso real de un alumno con Parálisis Cerebral y Discapacidad Intelectual asociada. Los objetivos de las actividades están centrados en el desarrollo de la autoconciencia, el autocontrol, la conciencia social y la toma de decisiones responsables. Mediante este proyecto se p...

  16. Interaccion de promastigotes de Leishmania donovani con celulas de exudado peritonial de ratones mediante la influencia de la quimotripsina

    OpenAIRE

    Arcay,Lucila; Bruzual,Elizabeth

    1986-01-01

    Se hace un estudio de la interacción de promastigotes de Leishmania donovani con células de exudado peritoneal de ratón (c e p) mediante la influencia de la quimotripsina. La adhesión de los promastigotes a las c e p fue terminal y marginal, y en observaciones hechas a partir de los 10 minutos de enfrentamiento, esta adhesión fue nula hasta los 30 minutos en el grupo tratado, y sólo a las das horas hubo un pequeño incremento (2.4%) con respecto al control. Se observa marcada disminución en to...

  17. Análisis genotípico en Vochysia guatemalensis Donn Smith (Vochysiaceae) mediante microsatélites

    OpenAIRE

    Rojas, Fabiana; Instituto Tecnológico de Costa Rica, Escuela de Ingeniería Forestal; Cartago, Costa Rica; Murillo-Gamboa, Olman; Instituto Tecnológico de Costa Rica, Escuela de Ingeniería Forestal; Cartago, Costa Rica; Aguilar, Gabriel; Universidad de Costa Rica; Rocha, Oscar; Kent State University, Ohio, USA; Araya-Valverde, Emanuel; Instituto Tecnológico de Costa Rica, Escuela de Ingeniería Forestal; Cartago, Costa Rica

    2012-01-01

    En el presente estudio se muestran los resultados del análisis de una colección de 62 clones de Vochysia guatemalensis ubicados en la zona norte y caribe de Costa Rica mediante el uso microsatélites (SSR), adaptados y validados de un grupo de marcadores existentes para Vochysia ferruginea. El material fueseleccionado de árboles plus procedente de varias poblaciones naturales y de pequeñas plantaciones. Se trabajó en el establecimiento de la huella genética de cada clon y en el agrupamiento de...

  18. Electrohilado de fibras coaxiales constituidas por polibutilenosuccinato (PBS, Bionolle) y polietilenglicol (PEG). Funcionalización mediante carga de drogas

    OpenAIRE

    2013-01-01

    En este proyecto se ha realizado un estudio de la preparación de micro y nanofibras de dos biopolímeros, Bionolle® (PBS) y polietilenglicol (PEG) de hidrofilidad diferente. Las fibras se obtuvieron mediante la técnica de electrospinning coaxial, en el que se han producido fibras posicionando cada uno de los polímeros en el shell y en el core y viceversa, así como la mezcla de ambos. Posteriormente se procedió a la caracterización estructural de las diferentes mezclas preparadas y al análisis ...

  19. Estudio y determinación de la población de bacterias en vino mediante epifluorescencia

    OpenAIRE

    Curiel Llanos, Laura

    2014-01-01

    Se ha procedido a la determinación de la población de bacterias mediante técnicas de epifluorescencia para tener un conocimiento más amplio y preciso de las bacterias viables y no viables en los procesos de elaboración de vinos. Dichas determinaciones son fundamentales a efectos de la calidad en la elaboración del vino. Se realiza la comparación con otras técnicas microbiológicas actualmente utilizadas. Máster en Técnicas Avanzadas en Química

  20. Diseño de un sistema de almacenaje de puros habanos controlando temperatura y humedad mediante termoelectricidad

    OpenAIRE

    2012-01-01

    El objetivo del proyecto es diseñar una cava para puros, controlando tanto temperatura como humedad. La temperatura se controla mediante células termoeléctricas (Peltier) en vez de sistemas por compresión que utilizan gases contaminantes. Para la humedad se utilizará un sistema exterior al habitáculo de la cava. En la actualidad se suele utilizar un humidificador dentro de cava. Incluso se desarrolla un sistema para regular la humedad en el caso de que suba demasiado en el habitáculo cosa ...

  1. Refrigeración de vacunas mediante una máquina frigorífica por efecto Peltier

    OpenAIRE

    2011-01-01

    El proyecto “Refrigeración de vacunas mediante una máquina frigorífica por efecto Peltier” estudia el control de la temperatura dentro de un volumen fijo de aire a fin de conservar vacunas. En este proyecto se describen las nuevas tecnologías refrigeración más demandadas y estudiadas en la actualidad en concreto de la tecnología de la termoelectricidad. La tecnología termoeléctrica en el campo de la refrigeración está basada en el principio del efecto Peltier que permite el bombeo de calor de...

  2. Caracterización estructural mediante elipsometría espectral de multicapas basadas en SiO2

    OpenAIRE

    Alonso, M. I.; Garriga, M.

    2000-01-01

    [ES] Se han caracterizado capas de óxidos subestequiométricos de silicio (SiOx) y estructuras gruesas de guías de onda mediante elipsometría espectral. Para ello ha sido necesario desarrollar un método de ajuste que permita analizar en detalle espectros complicados de elipsometría, como los obtenidos en estructuras multicapa gruesas (~10 μm) típicas de dispositivos modernos de óptica integrada. La elipsometría es la técnica más adecuada para la caracterización detallada de esas estru...

  3. Materiales poliméricos biodegradables preparados mediante procesado termomecánico a partir de mezclas gluten/plastificante

    OpenAIRE

    Zárate Ramírez, Lidia Soledad

    2011-01-01

    El objetivo del presente trabajo consiste en estudiar el efecto de la adición de diferentes agentes en la formulación de bioplásticos obtenidos a partir de gluten mediante mezclado y moldeo por compresión o extrusión, sobre las propiedades viscoelásticas, mecánicas y de absorción de agua de tales bioplásticos. Los resultados aquí obtenidos podrían contribuir a sentar las bases para futuras aplicaciones de estos materiales biodegradables. Para alcanzar este objetivo la investigación abordará l...

  4. Monitorización de la calidad del aire en tiempo real mediante el procesamiento de eventos complejos

    OpenAIRE

    Fernández de la Rosa, Iván

    2015-01-01

    El pilar fundamental de este TFG girará entorno a la monitorización de la calidad del aire en tiempo real, en el que se apoyará el objetivo principal, el cual consiste en la constante información y adecuación de dicha calidad del aire a determinados grupos de riesgo mediante el uso de tecnología aplicada al hogar. Para poder llevar acabo dicho propósito, se ideará una arquitectura que integre tanto Service-Oriented Architecture (SOA) como Complex Event Processing (CEP) cuyas principales fu...

  5. La representación del conocimiento especializado mediante las marcas de especialidad: del DRAE-1992 al DRAE-2001

    OpenAIRE

    Antoni Nomdedeu Rull

    2007-01-01

    En los últimos tiempos se han sucedido los análisis relacionados con la inclusión y la representación de los distintos campos del saber en la lexicografía general. En tal contexto, este estudio analiza la representación de los diferentes campos del saber en las dos últimas ediciones del «Diccionario de la lengua española» (DRAE), de la Real Academia Española, mediante la observación de las acepciones que incorporan marcas de especialidad y el estudio de las informaciones proporcionadas por la...

  6. Innovador tratamiento de heridas abdominales complejas mediante terapia de presión negativa con instilación intermitente

    Directory of Open Access Journals (Sweden)

    A. García-Ruano

    2013-09-01

    Full Text Available Describimos nuestra experiencia en el tratamiento de heridas abdominales postquirúrgicas dehiscentes y contaminadas con exposición de material protésico, mediante terapia de presión negativa con instilación intermitente de soluciones tópicas. Este dispositivo nos permitió el rescate de pacientes pluripatológicos evitando una reintervención compleja de alta morbi-mortalidad y facilitando el cierre de la herida, además con conservación de la malla en la mayor parte de los casos.

  7. La exploración del reflejo vestíbulo-ocular mediante el test de lectura

    OpenAIRE

    1999-01-01

    El test de lectura consiste en pedir al paciente que lea un texto mientras se le mueve la cabeza a uno y otro lado a una frecuencia de al menos 2 Hz. Si el reflejo vestíbulo-ocular (RVO) no tiene fallo alguno, será capaz de estabilizar la mirada y el paciente podrá leer correctamente. Si el RVO es defectuoso, la lectura se volverá imposible. Para demostrarlo, hemos valorado el RVO a una serie de 30 pacientes mediante el VAT, se han clasificado según el test de lectura y comparado los resultad...

  8. Un ambiente para evaluación de sistemas de software mediante métricas clásicas

    OpenAIRE

    Pesado, Patricia Mabel; De Giusti, Armando Eduardo; Zorzano, A.C. J.; Borroni, A. C. C.

    1996-01-01

    Se presenta un ambiente para evaluación de sistemas de software mediante recolección de métricas y estimaciones. El objetivo principal es conformar una línea base a partir de mediciones efectuadas sobre desarrollos ya implementados que sirva de información para futuras estimaciones. Sin duda las mejores estimaciones surgen del análisis de proyectos similares efectuados en la organización. Las experiencias recogidas de desarrollos análogos realizados por otras organizaciones pueden servi...

  9. Nivel de infección de helicobacter pylori mediante la técnica de ELISA

    OpenAIRE

    Moromi Nakata, Hilda; Prof. Asociado a D.E. instituto de Investigación Estomatológica Fac. Odontología UNMSM.; Calle Espinoza, Sonia; Prof. Asociado a D.E. Lab Miciobiología. Fac. Med Vet UNMSM.; Martínez Cadillo, Elba; Prof. Auxiliat a TP D~to. Ciencias Básicas. Lab. Microbiología Fac. Odontol. UNMSM.; Villavícencío Gastelú, Jorge; Prof, Asociado a TC Dpto. Médico Quirúrgico. Fac. Odontol UNMSM.; Zambrano de la Pena, Sonia; Prof. Asociado a TP Dpto. Médico-Quirúrgico. Sccción de Periodoncia. Fac. Odontol. UNMSM.

    2014-01-01

    En base a los resultados obtenidos sobre el hallazgo de Helicobacter pylori en los estudios precedentes, en Placa Dental, y en pacientes con gi. ngivitis y enfermedad periodontal, (Moronn, 1999 y 2000, respectivamente), se considero necesario implementar este proyecto, mediante la determinación de los niveles de infección a través de la presencia de anticuerpos específicos. En este caso el estudio abarcará una población de estudio más amplia, constituida por una muestra poblacional de estudia...

  10. Evaluación de la Escritura Mediante Rúbrica en la Educación Primaria en México

    OpenAIRE

    Luis Ángel Contreras Niño; Manuel González Montesinos; Erick Urías Luzanilla

    2009-01-01

    Se presentan resultados de un estudio evaluativo cuyo objetivo fue caracterizar mediante una rúbrica los escritos producidos por 3,151 niños y niñas mexicanos al egresar de la educación primaria en el estado de Baja California/MX. Se describe la rúbrica utilizada y los procedimientos para capacitar en su uso a 31 jueces especialistas en lenguaje, así como para estimar la confiabilidad de sus juicios sobre los escritos. Se describen también las puntuaciones, por nivel de logro, en cada uno de ...

  11. Desarrollo de un refuerzo Montante/Pilar A para automoción mediante nuevos procesos de conformado en caliente

    OpenAIRE

    Berto, Sébastien

    2009-01-01

    El proyecto tiene como objetivo el estudio de un refuerzo montante A, pieza estructural, fabricado mediante un nuevo proceso de conformado por soplado en caliente, y su posible integración en un coche. Esta pieza debería integrarse al nuevo modelo de Seat Leon, por eso se tendrá que definir conjuntamente con el área de Desarrollo Carrocería del Centro Técnico de SEAT y el proveedor Linde Wiemann una geometría tanto encajable, como fabricable. La elaboración y la evolución de...

  12. Generación de experiencias visuales en ciegos mediante estimulación táctil repetitiva

    OpenAIRE

    Tomás Ortiz; Santos, Juan M.

    2012-01-01

    Estudios recientes de nuestro laboratorio han establecido que se puede generar una activación estable del córtex visual en ciegos mediante entrenamiento táctil pasivo prolongado. Esta neuroplasticidad cortical se acompaña en un 40% de los participantes invidentes de sensaciones visuales, así como de una mayor rapidez en el reconocimiento táctil de información espacial tales como líneas, iconos o imágenes, y es crucial para su correcta interpretación. Estos hallazgos pueden ser útiles para el ...

  13. Optimización de la calidad de mallas de elementos finitos mediante cambios localizados de topología

    OpenAIRE

    2009-01-01

    Se presenta un método que mediante cambios localizados en la topología de una dada malla tridimensional de elementos finitos, logra mejorar la calidad de sus elementos, y en especial la de aquellos con volumen nulo o negativo. Se describirá el algoritmo general y se mencionarán algunos puntos importantes a tener en cuenta cuando la malla presenta estos elementos de volumen negativo. Por último se muestra el comportamiento del método al utilizarlo como optimizador de mallas ya generadas y s...

  14. Valorización de Biomasa de origen vegetal mediante procesos térmicos y termoquímicos.

    OpenAIRE

    López González, Diego

    2013-01-01

    En particular, esta Tesis Doctoral tiene como objetivo la evaluación de los principales procesos de conversión termoquímica de biomasa, principalmente pirólisis, combustión y gasificación, mediante el sistema experimental de termobalanza acoplada a un espectrómetro de masas. Adicionalmente, se estudió la degradación de fluidos de intercambio de calor en su aplicación en plantas termosolares de concentración. Los procesos de conversión termoquímica de biomasa son los procesos más interesan...

  15. Generación de experiencias visuales en ciegos mediante estimulación táctil repetitiva

    OpenAIRE

    Tomás Ortiz; Santos, Juan M

    2012-01-01

    Estudios recientes de nuestro laboratorio han establecido que se puede generar una activación estable del córtex visual en ciegos mediante entrenamiento táctil pasivo prolongado. Esta neuroplasticidad cortical se acompaña en un 40% de los participantes invidentes de sensaciones visuales, así como de una mayor rapidez en el reconocimiento táctil de información espacial tales como líneas, iconos o imágenes, y es crucial para su correcta interpretación. Estos hallazgos pueden ser útiles para el ...

  16. Fractura-luxación posterior de hombro tratada mediante la técnica de McLaughlin

    OpenAIRE

    Cubillo Martin, A.; Vicente Herrera, E.; Riquelme Arias, G.; Álvarez, C.; Rodriguez Álvarez, J.

    1996-01-01

    Se presenta un caso de luxación posterior de hombro, con fractura por impactación de la cabeza humeral, ocasionada por crisis convulsivas. Estas lesiones no son fáciles de reconocer en los primeros momentos, y en nuestro caso se precisó de un estudio radiográfico en distintas proyecciones y una TAC. Se realizó tratamiento quirúrgico mediante la técnica original de McLaughlin. A los 7 meses de la intervención el paciente ha recuperado casi totalmente la movilidad en dicho hombro...

  17. Producción de joyería fabricada mediante impresión 3D

    OpenAIRE

    GUILLÉN FERNÁNDEZ, ANA

    2015-01-01

    [ES] PRODUCCIÓN DE JOYERÍA DISEÑADA PARA SU IMPRESIÓN EN 3D [Es] Este proyecto trata sobre la fabricación directa de joyería mediante la impresión 3D con los materiales plásticos ABS y PLA. Se ha buscado una forma de utilizar el prototipo creado por la impresora como pieza definitiva, y para ello se han explorado diferentes posibilidades en colgantes, collares, pendientes, anillos, pulseras y broches. Las diferentes piezas se han diseñado con métodos digitales, utilizando el modelado 3D, e...

  18. Caracterización térmica del edificio 1C (UPV) mediante termografía infrarroja

    OpenAIRE

    GARCÍA MORATALLA, IRENE

    2014-01-01

    [es] Este Proyecto Fin de Grado pretende impulsar la utilización de la tecnología termográfica y evaluar la capacidad que posee esta técnica, no destructiva, para detectar y reconocer anomalías, defectos y lesiones en edificios. Se ha tratado de caracterizar un edificio dentro la Universidad Politécnica de Valencia mediante unas fichas que recogen distintos datos. Ante unos resultados positivos se ha llegado a la realización de un breve estudio analítico del modo en que varias empresas actual...

  19. Estudio numérico del problema geotérmico de Elder mediante FAHET : influencia del Rayleigh

    OpenAIRE

    Martínez Martínez, Valentín

    2014-01-01

    Los objetivos del presente Trabajo de investigación son los siguientes: 1. Describir los fundamentos teóricos que rigen los procesos de flujo de fluidos con transporte de calor. 2. Describir el estado del arte del problema de Elder. 3. Conocer y diseñar mediante FAHET los modelos de red con los que abordar el problema de Elder. 4. Experimentar las posibilidades del programa FAHET, así como el resto de programas en los que se apoya tanto para hallar la solución numérica del m...

  20. Un estudio acerca del desarrollo de videojuegos mediante el motor gráfico Unity 3D

    OpenAIRE

    Gil Romero, Eva

    2014-01-01

    El objetivo principal de este proyecto se centra en el estudio del desarrollo de videojuegos mediante el motor gráfico Unity 3D. Para ello, el proyecto aborda : i) la descripción de las funcionalidades y capacidades de cada una de las herramientas incluidas en el motor, ii)la descripción del papel que juega cada herramienta en el proceso de desarrollo de un videojuego y iii) el desarrollo de un pequeño prototipo de videojuego con este motor para ilustrar su utilización en este contexto.

  1. Eficacia del tratamiento de la cefalea tensional mediante técnicas neurodinámicas y de tejido blando

    OpenAIRE

    Ferragut Garcías, Alejandro

    2016-01-01

    Contexto. La cefalea tensional (CT) es una patología muy frecuente, cuya sintomatología causa un gran impacto social y económico. Sin embargo, la escasez de estudios de alta calidad hace que el desarrollo y eficacia de tratamientos no sean claros. Objetivos. Analizar la eficacia de un protocolo de tratamiento mediante técnicas de tejido blando y/o de técnicas neurodinámicas en pacientes con cefalea tensional. Material y métodos. Noventa y siete participantes de entre 19 y 60 años, co...

  2. Valorización de Biomasa de origen vegetal mediante procesos térmicos y termoquímicos.

    OpenAIRE

    López González, Diego

    2013-01-01

    En particular, esta Tesis Doctoral tiene como objetivo la evaluación de los principales procesos de conversión termoquímica de biomasa, principalmente pirólisis, combustión y gasificación, mediante el sistema experimental de termobalanza acoplada a un espectrómetro de masas. Adicionalmente, se estudió la degradación de fluidos de intercambio de calor en su aplicación en plantas termosolares de concentración. Los procesos de conversión termoquímica de biomasa son los procesos más interesan...

  3. OXIDACIÓN DE MATERIA ORGÁNICA PERSISTENTE EN AGUAS RESIDUALES INDUSTRIALES MEDIANTE TRATAMIENTOS ELECTROQUÍMICOS

    OpenAIRE

    Ivonne Linares-Hernández; Verónica Martínez-Miranda; Carlos Barrera-Díaz; Sergio Pavón-Romero; Lina Bernal-Martínez; Violeta Lugo-Lugo

    2011-01-01

    El objetivo de esta investigación fue implementar un sistema electroquímico, mediante tratamientos de electrocoagulación, empleando electrodos de hierro y oxidación anódica directa (OAD), empleando electrodos de diamante dopados con boro (DDB), para tratar aguas residuales provenientes de una planta de tratamiento que recibe las descargas de 144 empresas de diferentes giros, de la zona industrial Toluca-Lerma, México. Los resultados de estos tratamientos, indicaron una remoción del 99% de la ...

  4. Modificación superficial de materiales poliméricos mediante fuentes de plasma frío

    OpenAIRE

    Encinas García, Noemí

    2012-01-01

    Esta tesis contiene artículos de investigación en anexo. En la presente Tesis Doctoral se ha desarrollado un procedimiento adecuado para la mejora en las propiedades adhesivas de superficies poliméricas a través del incremento en capacidad de mojado de las mismas, mediante tratamiento con antorcha de plasma atmosférico trabajando con aire. Para ello, se evalúan las condiciones óptimas de tratamiento de las muestras en función de los parámetros de tiempo de exposición y distancia antorcha-m...

  5. Modelización automatica de problemas de redes de distribución mediante tecnología JAVA

    OpenAIRE

    Patricia Gómez García; Angel Cobo Ortega

    2002-01-01

    Esta comunicación presenta un programa informático desarrollado para el tratamiento gráfico de problemas de redes de distribución y su modelización de forma automática en los lenguajes de algunos de los programas de investigación operativa más conocidos y utilizados. El programa ha sido desarrollado utilizando el lenguaje de programación Java, por lo que puede ser utilizado a través de Internet. Mediante una interfase gráfica muy intuitiva, el usuario puede diseñar la red de transporte, ...

  6. Estudio del subsuelo mediante técnicas de prospección geofísica

    OpenAIRE

    PLAMADEALA, ARTIOM

    2016-01-01

    [ES] El presente trabajo consiste en la realización de una prospección geofísica en la Plaza de las Fuentes en el municipio Torrente. Se pretende buscar restos arqueológicos de la antigua muralla. Se requiere una técnica no invasiva, y por lo tanto se ha empleado el georradar. Plamadeala, A. (2016). Estudio del subsuelo mediante técnicas de prospección geofísica. http://hdl.handle.net/10251/70856. TFGM

  7. VGEAR : Sistema de adquisión de datos mediante OBD-II y bus ISO 14230

    OpenAIRE

    Zayas García, Daniel Javier

    2015-01-01

    Este proyecto consiste en la conexión al bus de diagnóstico ODB de una motocicleta Suzuki Intruder M800 mediante los estándares ISO 14230 e ISO 15031 obteniendo diferentes parámetros: velocidad, marcha y RPM del motor. Aquest projecte consisteix en la connexió al bus de diagnòstic ODB d'una motocicleta Suzuki Intruder M800 mitjançant els estàndards ISO 14230 i ISO 15031 obtenint diferents paràmetres: velocitat, marxa i RPM del motor. Bachelor thesis for the Telecommunication Technologie...

  8. Adquisición de una discriminación operante mediante observación en palomas

    OpenAIRE

    Javier Nieto; Rosalba Cabrera

    1993-01-01

    Se reportan los resultados de dos experimentos que fueron diseñados para evaluar si las palomas aprenden respuestas discriminadas mediante la observación de un modelo. En el primer experimento se utilizaron tres grupos de palomas observadoras. El grupo de Discriminación Sin Errores observó a un modelo responder ante el color positivo y recibir reforzamiento, y nunca responder ante el color negativo. El grupo Discriminación Con Errores observó que picar el color positivo era seguido d...

  9. Evaluación de la hipótesis de conectoma mediante pruebas neuropsicológicas

    OpenAIRE

    López Hernández, Beatriz

    2016-01-01

    Objetivos: Evaluar la sensibilidad de las pruebas neuropsicológicas para diferenciar patrones entre pacientes con epilepsia del lóbulo frontal (ELF) y pacientes con epilepsia del lóbulo temporal con esclerosis mesial (ELTM). Metodología: 60 pacientes epilépticos lesionales (30 con ELF y 30 con ELTM) fueron valorados mediante una completa batería neuropsicológica incluyendo pruebas habitualmente relacionadas con la memoria (subtest de la Escala de Memoria Wechsler- Revisada, Test de denominaci...

  10. Mejora de la biodisponibilidad nutricional mediante el proceso biotecnológico de germinación en Vigna unguiculata

    OpenAIRE

    Aguilera, Yolanda; Benitez, Vanesa; Jiménez, Tania; Calvo, Sara; Díaz, María Felicia; Martín-Cabrejas, María A.

    2011-01-01

    Existe gran parte de la población humana y animal que se encuentran en países en vía de desarrollo y sufren malnutrición proteica. La búsqueda de alimentos e ingredientes alternativos tanto para el hombre como para el ganado resulta esencial. El objetivo de este estudio fue evaluar la mejora de la biodisponibilidad nutricional mediante el proceso biotecnológico de germinación en Vigna unguiculata. Los resultados muestran que esta legumbre exhibe importantes niveles de proteínas (>27mg/100g) y...

  11. Tratamiento quirúrgico de las fracturas vertebrales mediante instrumentación segmentaria Cotrel-Dubousset

    OpenAIRE

    Martín Benlloch, Juan Antonio; Segura, F. (Félix); Soler Heredia, A.; Laguia, M.

    1993-01-01

    Los autores presentan su experiencia personal en el tratamiento quirúrgico de las fracturas vertebrales torácicas y lumbares. Han sido incluidos en el estudio 27 pacientes operados entre 1988 y 1991, todos ellos estabilizados mediante la instrumentación Cotrel Dubousset. Excepto tres pacientes, todos fueron intervenidos por vía posterior. Se realiza un estudio de los resultados obtenidos desde el punto de vista radiológico (índice sagital, porcentaje de ocupación de canal), fun...

  12. Estimación de la vulnerabilidad de acuíferos mediante un modelo cuantitativo ­ hydrus 1d

    OpenAIRE

    Ríos Rojas, Liliana; Vélez Otálvaro, María Victoria

    2009-01-01

    En el presente trabajo se efectúa una estimación y zonificación de la vulnerabilidad a la contaminación del agua subterránea del sector sur del acuífero del Valle del Cauca, mediante el modelo HYDRUS­1D que representa los fenómenos de flujo y transporte en la zona no saturada (ZNS). Se comparan los resultados con los obtenidos usando diferentes métodos de índice y superposición. También se simula un evento de contaminación usando el modelo Visual MODFLOW para la zona satura...

  13. Evaluación anatómica del piso de boca mediante resonancia magnética

    OpenAIRE

    Quispe Huarcaya, Magaly; Sovero Gaspar, Abell; Gutiérrez Ventura, Fredy

    2016-01-01

    El presente artículo de revisión fue realizado para reconocer las estructuras anatómicas del piso de boca mediante imágenes de resonancia magnética; esta es una de las regiones más delicadas de la cavidad oral, constituidasolamente por tejidos blandos, muchos de ellos vasculares, glandulares y nerviosos, carece de reparos anatómicos óseos siendo susceptible a patologías que deben ser diagnosticadas por el estomatólogo. Se considera a la resonancia magnética el estudio de elección para los tej...

  14. Catalizadores jerárquicamente organizados mediante nanodispersión por vía seca

    OpenAIRE

    Fernández Lozano, José Francisco; Rubio Marcos, Fernando; Calvino Casilda, Vanesa; Bañares González, Miguel Ángel

    2010-01-01

    La presente invención se refiere a un procedimiento para la obtención de catalizadores mediante nanodispersión y anclaje de nanopartículas o cluster nanoparticulados de tamaño inferior a 100 nm sobre soportes submicrónicos o micrométricos empleando un proceso de dispersión en vía seca de baja energía. Los catalizadores preparados a temperatura ambiente están caracterizados por presentar nuevas intercalas soporte-nanopartícula capaces de generar nuevas superficies react...

  15. Readaptación física en futbolistas mediante vibraciones mecánicas e hipoxia

    OpenAIRE

    Urdampilleta, A.; Álvarez-Herms, J.; Martínez-Sanz, J. M.; Corbi, F.; Roche, E

    2014-01-01

    Las acciones explosivas en el fútbol se triplican respecto a los años 60, llegando a las 200-215 acciones explosivas/partido. Esto supone que la potencia muscular y la capacidad de recuperación sean factores limitantes, pudiendo ser frecuentes las lesiones musculares. Durante la lesión se pierden las cualidades condicionales, menos cuanto más corto sea este periodo. Existen diversos métodos para la mejora de la fuerza y capacidad de recuperación mediante las plataformas vibratorias y la hipox...

  16. Representación de datos en metereología mediante gráficos animados: episodios de lluvia

    OpenAIRE

    Navarro, Jorge Luis

    1987-01-01

    Los campos escalares, bidimensionales y dependientes del tiempo, se representan generalmente en Meteorología mediante una sucesión de imágenes o análisis separados con una interdistancia temporal adecuada. Esta técnica alcanza su máximo desarrollo en lo que se conoce como gráfico animado. En el presente artículo se describen brevemente los procesos que, a partir de los datos, culminan con la generación de un análisis, imagen o fotograma, particularizando al caso de episodios de lluvia. Tra...

  17. Determinación de la conductividad térmica en nanohilos mediante efecto fotoacústico

    OpenAIRE

    Solís Martín, David

    2010-01-01

    El presente proyecto, tiene como objetivo el diseño e implementación de un sistema de instrumentación electrónico, capaz de determinar la conductividad térmica de nuevos materiales, mediante el efecto fotoacústico. La medición fotoacústica se enmarca dentro de los análisis no destructivos y presenta una clara ventaja en la medición de matrices nanoestructuradas dado que no precisa de contacto mecánico con la muestra. La celda fotoacústica desarrollada se ha optimizado acorde al objetivo de ha...

  18. Análisis de la demanda de transporte interinsular mediante modelos avanzados de elección discreta

    OpenAIRE

    Grisolía Santos, José María

    2006-01-01

    Programa de doctorado: Economía aplicada [ES]En esta tesis se analiza el transporte interurbano de pasajeros entre Gran Canaria y Tenerife mediante el diseño y aplicación de una encuesta de preferencias reveladas que permitió la obtención de 1011 observaciones. A partir de estos datos se estimaron diversos modelos de elección discreta que han permitido calcular el valor subjetivo del tiempo de los viajeros y de los diferentes tipos de viaje, así c...

  19. DISTRIBUCIÓN ESPACIAL DE LA TUBERCULOSIS EN ESPAÑA MEDIANTE MÉTODOS GEOESTADÍSTICOS

    OpenAIRE

    2009-01-01

    Fundamentos: La incidencia de tuberculosis (TB) se ha asociado a factores, epidemiológicos y sociales. En España, la TB es una enfermedad de declaración obligatoria e individualizada. Las tasas de TB respiratoria experimentan un descenso constante en los últimos años. El objetivo es valorar la asociación entre la morbilidad por TB respiratoria y variables socioeconómicas y epidemiológicas así como su distribución espacial mediante métodos geoestadísticos. Método: Las tasas de incidencia se...

  20. Simulación de sistemas Multi-Agente mediante un esquema Monte Carlo basado en ecuaciones diferenciales ordinarias

    OpenAIRE

    Zulueta Guerrero, Ekaitz; González González, Asier; Lopez Guede, José Manuel; Calvo Gordillo, Isidro

    2011-01-01

    [ES] En este trabajo se expone una metodología para modelar un sistema Multi-Agente (SMA), para que sea equivalente a un sistema de Ecuaciones Diferenciales Ordinarias (EDO), mediante un esquema basado en el método de Monte Carlo. Se muestra que el SMA puede describir con mayor riqueza modelos de sistemas dinámicos con variables cuantificadas discretas. Estos sistemas son muy acordes con los sistemas biológicos y fisiológicos, como el modelado de poblaciones o el modelado de enfermedades epid...

  1. Localización mediante SIG de zonas potencialmente truferas en la provincia de León

    OpenAIRE

    2008-01-01

    La trufa negra (Tuber melanosporum) es un hongo que forma una asociación mutualista con determinadas especies hospedadoras que determina beneficios para ambos, disponibilizando carbohidratos para el hongo y mejorando la nutrición del hospedador mediante un aumento en la absorción de elementos químicos del suelo. La aptitud del terreno para la producción trufera viene determinada por sus condiciones geológicas, edafológicas (textura y composición química), climáticas (precipitación y...

  2. Estudio nutricional de universitarios cordobeses mediante valoración antropométrica, bioquímica y alimentaria

    OpenAIRE

    León Fernández, María José

    2011-01-01

    Se ha valorado la situación nutricional de 85 universitarios de ambos sexos entre 18 y 35 años mediante el estudio de la ingesta, las medidas antropométricas y los indicadores hematológicos y de bioquímica sanguínea. Con este estudio se pretende conocer la situación del colectivo universitario para diseñar actuaciones en nutrición con la finalidad de mejorar el estado de salud y prevenir futuros problemas. Respecto a la valoración antropométrica destaca que en los varones hay mayores cifra...

  3. Refuerzo de puentes por cambio de esquema estructural: optimización mediante algoritmo genético

    OpenAIRE

    Valenzuela, Matías A.; Casas Rius, Joan Ramon

    2011-01-01

    La presente comunicación entrega un estudio detallado del proceso constructivo y de tesado para el refuerzo de puentes con tipología longitudinal de vigas continuas convirtiéndolos en puentes en arco atirantado (tipo network). Se presentan las etapas básicas propuestas en aspectos de construcción, se establecen las hipótesis del estudio de tesado e implementa el método de tesado a partir de la optimización automatizada mediante algoritmos genéticos, definiendo criterios como: variables de ...

  4. Evaluación del perfil de ácidos grasos de carne de vacuno mediante espectroscopia MIR

    OpenAIRE

    2015-01-01

    El objetivo de este trabajo de fin de carrera es la evaluación del perfil de ácidos grasos de la carne de vacuno, con especial atención a los de interés nutricional (ácido linoleico conjugado, CLA por sus siglas en inglés, y lino) en carne y grasa intramuscular de ternero frisón mediante espectroscopia de infrarrojo medio (MIR por sus siglas en inglés). Para la realización de este trabajo se han empleado muestras de 24 terneros frisones. Estas muestras se encuentras dividida...

  5. Medición de contaminación mediante UAV (Vehículo Aéreo no Tripulado)

    OpenAIRE

    Edwin José Vera-Rozo

    2016-01-01

    Este artículo presenta un procedimiento experimental cuyo objetivo es obtener la medición de contaminación en un relleno sanitario (basurero) mediante un Vehículo Aéreo no Tripulado (UAV); La metodología utilizada consistió en realizar un procedimiento detallado para la instrumentación de UAV, el cual fue equipamiento con un sistema para la captura y almacenamiento de datos referente a las variables medidas en tiempo real, el cual posteriormente se puso en vuelo y luego se realizo el procesam...

  6. Tratamiento de las osteitis diafisarias de tibia mediante sinostosis tibioperonea y resección ósea

    OpenAIRE

    Fernández Sabaté, Alfons; Riu Labrador, R.; Moreta Munujos, D.; Cáceres Palou, Enrique

    1984-01-01

    El tratamiento de las osteítis crónicas de la diáfisis tibial obliga a menudo a practicar una amplia resección para alcanzar el tejido óseo sano y esta exigencia operatoria debilita la diáfisis. Para obviar la fractura operatoria o posterior por fatiga y para facilitar la resección sin temor se ha utilizado una táctica quirúrgica consistente en una solidarización tibioperonea que construye un residente eje en la pierna ya sea mediante injerto intertibioperoneo ya con peroné protibia de Zanoli...

  7. Tratamiento de las osteitis diafisarias de tibia mediante sinostosis tibioperonea y resección ósea

    OpenAIRE

    Fernández Sabaté, Alfons; Riu Labrador, R.; Moreta Munujos, D.; Cáceres Palou, Enrique

    1984-01-01

    El tratamiento de las osteítis crónicas de la diáfisis tibial obliga a menudo a practicar una amplia resección para alcanzar el tejido óseo sano y esta exigencia operatoria debilita la diáfisis. Para obviar la fractura operatoria o posterior por fatiga y para facilitar la resección sin temor se ha utilizado una táctica quirúrgica consistente en una solidarización tibioperonea que construye un residente eje en la pierna ya sea mediante injerto intertibioperoneo ya con peroné protibia de Zanoli...

  8. Reconstrucción dentoalveolar con implantes dentales mediante la técnica de levantamiento de seno maxilar

    OpenAIRE

    Granados Páucar, Luis; Ikeda Artacho, María Cristina; Sueldo Gálvez, Luis

    2015-01-01

    La colocación de implantes en el sector posterosuperior con frecuencia debe enfrentar casos de atrofi a de rebordemaxilar y neumatización del seno maxilar, para los cuales una de las técnicas hoy en día empleada es la elevacióndel piso del seno maxilar mediante el aumento vertical del piso antral. Se reporta el caso de un paciente femeninode 60 años con exodoncias previas, diagnóstico de edentulismo parcial bimaxilar y neumatización del senomaxilar derecho. Se realizó como tratamiento el leva...

  9. Estudio del crecimiento de Prioria copaifera (Caesalpinaceae mediante técnicas dendrocronológicas

    Directory of Open Access Journals (Sweden)

    Jorge Andrés Giraldo Jiménez

    2011-12-01

    Full Text Available El cativo (Prioria copaifera G. forma bosques dominados por la especie llamados cativales. Durante más de 70 años el cativo ha sido la base principal de la industria maderera en el Darién colombiano, siendo, por su alta productividad y dominancia uno de los bosques tropicales más fáciles de ordenar sosteniblemente. El propósito de esta investigación es modelar el crecimiento del diámetro y el volumen del cativo en función de la edad así como las tasas instantáneas, medias y relativas, empleando anillos de crecimiento. La anualidad de los anillos se demostró usando el efecto de las bombas nucleares y mediante cofechado. Con base en los anillos de crecimiento radiales se modelo el crecimiento del diámetro y del volumen. Esta especie alcanza 40cm de diámetro en 90 años y presenta crecimiento medio de 0.31cm/año. El lapso vital de la especie es de aproximadamente 614 años. El incremento corriente anual máximo del volumen a los 90 años es 0.033m3/año, e iguala al incremento medio a los 145 años con 0.018m3/año. La tasa media de crecimiento absoluta es 0.021m3/año. Los resultados aquí obtenidos sirven como base técnica para el manejo sostenible de los cativales.A growth study of Prioria copaifera (Caesalpinaceae using dendrochronological techniques. The Cativo (Prioria copaifera forms very homogeneous forests called cativales in the flooded plains of some rivers from Costa Rica to Colombia. For over 70 years Cativo has been the main base of the timber industry in the Colombian Darien area. Because of high productivity and high-dominance of Cativo trees, they represent one of the most prone tropical forests for sustainable forest management. The objective of this research is to model diameter and timber volume growth and growth rates (absolute, mean and relative of Cativo as a function of age, using tree ring data derived from dendrochronologycal techniques. We evaluated the annual nature of the tree rings by radiocarbon

  10. Corte mediante láser de elementos estructurales de mullita-alúmina

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    Quintero, F.

    2006-04-01

    Full Text Available The application of high power lasers for cutting of structural ceramics is a process under development exhibiting great advantages in comparison with conventional machining techniques. However, an in depth analysis of the process is necessary before its industrial implementation in order to avoid eventual drawbacks and to optimize the results. The most relevant results from the study of laser cutting of structural elements composed of a mullite matrix with a dispersed phase of alumina are presented in this work. The results obtained employing a CO2 laser operated in CW mode are compared with those obtained using a free-running Nd:YAG in pulsed mode. In both cases, the effect of the operation parameters with stronger influence on the cut quality is analyzed. At the same time, the application of a conventional cutting head is compared with a new one specifically designed to optimize the cut quality. Several quantifiable parameters were employed to characterize the quality of the cuts with the aim of performing a systematic assessment of the results which allows the optimization of the process. The results obtained show a clear improvement of the cut quality as the new cutting head is employed.

    La aplicación del láser de alta potencia para el corte de cerámicas estructurales es un proceso en desarrollo que ofrece excepcionales ventajas en comparación con las técnicas convencionales de mecanizado. Sin embargo, como paso previo a su implementación industrial se hace necesario realizar un análisis profundo del proceso que permita evitar los eventuales inconvenientes y optimizar los resultados. En este trabajo se presentan los resultados más relevantes del estudio del corte mediante láser de elementos estructurales compuestos de una matriz de mullita con una fase dispersa de alúmina. Se comparan los resultados obtenidos al emplear un láser de CO2 operado en modo continuo con los correspondientes a la utilización de un láser de Nd

  11. Falácia naturalista e naturalismo moral: do é ao deve mediante o quero

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    Adriano Naves de Brito

    2010-06-01

    Full Text Available O objetivo deste texto é discutir alguns argumentos contra a aceitação da falácia naturalista. Pretende-se mostrar aspectos que parecem corretos na argumentação dos que recusam aquela falácia, e, a despeito de seus acertos, demonstrar que estão fundamentalmente errados. O eixo para este desfecho é a reformulação da falácia em termos da recusa da implicação entre o ser e o querer. Espera-se, assim, tornar mais claras as relações - e a ausência delas - entre verdade e moral, bem como abrir espaço para a defesa de um naturalismo moral não comprometido com a existência de fatos morais. Este texto está dividido em cinco partes. Na primeira, apresenta-se a falácia naturalista de acordo com Moore; na segunda, distingue-se essa entre as formulações de Moore e de Hume; na terceira, apresentam-se objeções à interdição da passagem do "é" ao "deve" e se formulam respostas a essas objeções; na quarta, reapresenta-se o problema da interdição entre "é" e "deve" e se focaliza uma solução alternativa a ele mediante o querer; e, finalmente, na quinta parte, elaboram-se algumas conclusões acerca do naturalismo e do realismo na moral.This article aims to discuss some arguments against the acceptance of the naturalistic fallacy. It intends to demonstrate that some aspects, which seem to be correct in the argumentation of those who refuse that fallacy, are, despite their pertinence in some points, fundamentally imprecise. In order to reach this outcome, the fallacy is reformulated in terms of the refuse of the implication between "being" and "the will". Therefore, it pretends to clarify the relations - and their absence - between truth and morality as well as enable a defense of a moral naturalism disengaged from the existence of moral facts. This text is divided in five parts. In the first one, I show the naturalistic fallacy according to Moore; in the second part, a distinction between Moore's and Hume's formulations of the

  12. Tribología de recubrimientos Cermet/NiCrBSi depositados mediante HVOF

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    Guilemany, J. M.

    2004-04-01

    Full Text Available This work consists on a deep tribological study of the WX system composed by a mechanical blend in different compositions of NiCrBSi and WC-12Co powders: 20% NiCrBSi (W2, 40% NiCrBSi (W4 y 60% NiCrBSi (W6. The coatings have been obtained by high velocity oxy-fuel process (HVOF. The measurements made by Ball-On-Disk test are: the friction coefficient is lower than 0.5 and the exchanged energy between the counterparts is under 10 KJ. To quantify the friction wear rate, the volume loss and the track depth, Scanning White Light Interferometry and SEM have been used. The track depth is proportional to the amount of NiCrBSi. A higher percentage of WC-12Co increases the friction wear resistance and decreases the abrasion wear rate (Rubber Wheel test. In all the coatings studied, no diffusion processes are found between the mixed phases, the adhesion between the coatings and the substrate is excellent, the porosity level is below 2% and an increase of microhardness of the coating due to a strengthening of the matrix produced by impacts of solid particles, takes place.

    El trabajo consta de un completo estudio tribológico del sistema WX que consiste en la mezcla mecánica de WC-12Co y NiCrBSi en diferentes proporciones: 20% NiCrBSi (W2, 40% NiCrBSi (W4 y 60% NiCrBSi (W6. Los recubrimientos se han obtenido por proyección térmica de alta velocidad (HVOF. Para todos los sistemas, el coeficiente de fricción es menor que 0.5 y la energía intercambiada entre el par friccionante y el recubrimiento es inferior a 10 KJ. Para cuantificar el desgaste por fricción se han utilizado la profundidad de la huella y el volumen perdido durante el ensayo, obtenidos mediante Interferometría de Barrido de Luz Blanca (SLWI y SEM. La profundidad de la huella sigue una evolución directamente proporcional al contenido en NiCrBSi. Un mayor contenido en WC-12Co aumenta la resistencia al desgaste por fricción y disminuye la velocidad de desgaste por abrasión (ensayo

  13. Modificación de la personalidad mediante una terapia cognitivo-conductual de afrontamiento al estrés

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    Julia Linares-Ortiz

    2014-01-01

    Full Text Available El objetivo de este estudio ha sido comprobar la posibilidad de modulación de variables de personalidad (tales como algunos de los Cinco Grandes Factores, o la personalidad resistente a través de la aplicación de un programa de afrontamiento del estrés. Para ello, han participado 26 personas del ámbito universitario con alto estrés percibido, distribuidas en dos grupos (grupo de tratamiento y grupo de control. Los instrumentos de evaluación seleccionados se clasificaron en dos grupos: variables psicológicas y emocionales y variables de personalidad. Los resultados encontrados mediantes un ANOVA de medidas repetidas mostraron que existía interacción momento x grupo en las variables optimismo, extraversión y responsabilidad, correspondientes al Modelo de los Cinco Grandes Factores, mostrando un incremento de las puntuaciones en estas variables en el grupo terapia y no encontrándose dicha interacción en el grupo control. Las puntuaciones en los componentes de neuroticismo, amabilidad, apertura a la experiencia y personalidad resistente no se modificaron en ninguno de los dos grupos. Este estudio apoya la idea de que modificando determinados parámetros emocionales relacionados con el estrés mediante terapia cognitivo-conductual se pueden ver modulados algunos factores de personalidad.

  14. Evaluación de la Escritura Mediante Rúbrica en la Educación Primaria en México

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    Luis Ángel Contreras Niño

    2009-01-01

    Full Text Available Se presentan resultados de un estudio evaluativo cuyo objetivo fue caracterizar mediante una rúbrica los escritos producidos por 3,151 niños y niñas mexicanos al egresar de la educación primaria en el estado de Baja California/MX. Se describe la rúbrica utilizada y los procedimientos para capacitar en su uso a 31 jueces especialistas en lenguaje, así como para estimar la confiabilidad de sus juicios sobre los escritos. Se describen también las puntuaciones, por nivel de logro, en cada uno de los rasgos de la escritura evaluados mediante la rúbrica. Además, se aportan evidencias relacionadas con la validez del examen que incluyó el ítem de ejecución que capturó las muestras de la escritura. Finalmente, se sugieren estudios cualitativos y cuantitativos que amplíen y profundicen los hallazgos encontrados en esta evaluación a gran escala.

  15. Producción de biodiesel de aceite crudo de palma mediante catálisis heterogénea

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    Fernando Cardeño

    2010-01-01

    Full Text Available Se estudió la producción de biodiesel de aceite crudo de palma mediante el uso de catalizadores heterogéneos ácidos y básicos para las etapas de preesterificación y transesterificación respectivamente. La pre-esterificación es necesaria para aceites con una acidez superior al 4% de ácidos grasos libres, ya que se evitan los problemas asociados con la formación de jabones. En ambas reacciones las variables analizadas fueron el tipo de catalizador, la temperatura y el tiempo. Se analizó la conversión del aceite a metilésteres usando cromatografía gaseosa y la estabilidad de los catalizadores mediante su reutilización. En la etapa de pre-esterificación se encontró que catalizadores ácidos del tipo resinas de poliestireno sulfonadas presentan conversiones superiores al 87% estabilidad para la esterificación de los ácidos grasos libres. Para la transesterificación se estudiaron como catalizadores heterogéneos, el carbonato de potasio libre y soportado en una matriz polimérica, obteniéndose con ambos conversiones superiores a 95 % de biodiesel; con el uso del soporte polimérico se disminuyó la velocidad de disolución de carbonato de potasio, permitiendo su reutilización hasta 10 reutilizaciones.

  16. Resistencia a la corrosión de recubrimientos electroquímicos de cromo y zinc mediante EIE

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    Magda Marcela Torres Luque

    2011-01-01

    Full Text Available Se ha estudiado el comportamiento frente a la corrosión de recubrimientos electrolíticos industriales de cromo y zinc mediante la técnica de espectroscopia de impedancia electroquímica en electrólito de NaCl al 3% en peso. Con el objetivo de determinar los mecanismos de protección y de falla de estos recubrimientos se ha correlacionado la resistencia a la polarización en el tiempo, con la morfología y la composición química. Para ello, los recubrimientos fueron caracterizados mediante microscopia electrónica de barrido, difracción de rayos X, espectroscopia de fotoelectrones de rayos X y espectroscopia de electrones Auger. Ambos recubrimientos muestran degradación en el tiempo de ensayo debido a los defectos presentes en su microestructura, sin embargo, el mecanismo de protección y, por tanto, de falla es diferente para cada sistema. Estos detalles son discutidos en esta investigación.

  17. Detección Molecular de Toxinas Termoestable y Termolabil de Escherichia coli mediante Hibridación

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    Isabel Arias B

    2002-10-01

    Full Text Available Objetivos: Identificar mediante el método de hibridización por colony blot las toxinas de Escherichia coli enterotoxigénica y relacionar los resultados con los serotipos encontrados. Materiales y métodos: se evaluaron todas las cepas de E. Coli recolectadas en el Hospital de Emergencias Pediátricas de Lima durante los meses de diciembre de 1998 - abril 1999. Se usaron dos sendas de ADN que identificaban el gen de la toxina termolábil (LT y el de la toxina termoestable (ST Para la detección de los serotipos se usaron 22 antisueros de diferentes categorías de E. coli. Resultados: se encontraron 233 cepas de E. coli, 27,9% de E. coli poseían el gen LT, 3,0% el gen ST y 1,3% tenían ambos. Conclusiones: los serotipos y la presencia de los genes LT y ST no necesariamente tienen relación, demostrándose que la identificación serológica es importante en el estudio epidemiológico de diarreas causadas por E. coli debiéndose confirmar la identificación de las categorías patogénicas mediante la detección de factores de virulencia.

  18. Manejo sostenible y sustentable de fincas productoras mediante procesos participativos en Sáchica, Boyacá

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    Ángel Eduardo Ramírez-Amaya

    2013-07-01

    Full Text Available Objetivo. Elaborar un proyecto de desarrollo sostenible y sustentable de fincas productoras mediante procesos participativos en el municipio de Sáchica, Boyacá. Materiales y métodos. La investigación se realizó con familias campesinas de la vereda Arrayán Alto, del municipio de Sáchica, Boyacá, mediante la metodología Investigación Acción Participativa (IAP, que se centra en la participación de las comunidades para elaborar propuestas concertadas con ellas. El trabajo se desarrolló en varias fases, que incluyeron un diagnóstico socioeconómico de las familias, capacitaciones y concientización en temas relacionados con la agricultura ecológica y de granjas integrales. Resultados. Se elaboró un plan de trabajo que permitió la construcción de un documento final que ha servido para el apoyo logístico o económico de las entidades gubernamentales locales para la instalación y plantación técnica del cultivo de gulupa con familias de la vereda Arrayán Alto.

  19. Control estadístico de la calidad de un servicio mediante Gráficas X y R

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    Alberto Isaac Pierdant Rodríguez

    2009-01-01

    Full Text Available Las empresas y organismos públicos que proporcionan servicios en México no utilizan frecuentemente técnicas cuantitativas para el control de calidad de dicho servicio, por lo que este trabajo representa una propuesta de control de calidad mediante herramientas simples de control estadístico de calidad. Existen diversas técnicas cualitativas y pocas técnicas cuantitativas como las gráficas, que nos permiten determinar si la prestación de un servicio se encuentra bajo control; es decir, verificar si la calidad está dentro de los estándares establecidos por la empresa o institución, o fuera de ellos. Este documento tiene como objetivo mostrar la mecánica del control de calidad de un servicio mediante dos tipos de gráficas de control para variables: la gráfica de control para medias de un proceso y la gráfica de control para variabilidad de procesos. Su aplicación permitiría identificar áreas de oportunidad e implementar mejoras en la calidad del servicio. El material ha sido elaborado empleando la hoja electrónica de cálculo Excel con la finalidad de proporcionar una herramienta práctica en esta materia.

  20. Sistema de Control mediante LabVIEW para analizar la Contaminación Lumínica Automotriz

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    Tatiana Carvajal

    2014-11-01

    Full Text Available Se ha desarrollado un sistema de control mediante LabView para analizar la contaminación lumínica automotriz en el medio ambiente. Algunos de los fenómenos analizados son: la irradiancia, espectros de emisión y potencia óptica. Los focos analizados fueron de tipo halógeno y LED, el estudio se llevó a cabo mediante un circuito electrónico similar al control de luces que poseen los carros. Los resultados obtenidos a través de la plataforma LabView indicaron que los focos halógenos de marca alemana, que son los más vendidos en el territorio ecuatoriano, no poseen una irradiación nociva como es en el caso de los LEDs, incluso así, tienen potencias exageradamente altas ocasionando deslumbramientos en la noche. Por su parte, los LEDs han demostrado tener una potencia baja, pero una alta intensidad de luz en las longitudes de onda de 430nm o 450nm, en los LEDs blanco y azul, respectivamente; siendo estas longitudes de onda las causantes de varios daños al sistema ecológico y a la salud cuando el sujeto es expuesto a largos periodos de tiempo

  1. Coloración Psicodélica de Imágenes Digitales mediante Lógica Difusa

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    Linda Sheriyn Rodríguez Castro

    2013-06-01

    Full Text Available En el presente artículo se propone un sistema de inferencia difusa para la manipulación del color en imágenes digitales con el propósito de modificar su estilo artístico. Se genera una combinación de colores diferente a la original de tal forma que produzca una nueva sensación visual. El sistema de lógica difusa propuesto, modela mediante conjuntos y reglas de inferencia difusas, la modificación de color considerando la modalidad artística denominada arte psicodélico, generando como resultado imágenes cuyos colores producen este efecto. En la segunda parte se afina el sistema de inferencia propuesto según datos empíricos determinados junto con expertos en el campo del arte, con el fin de obtener un mejor ajuste a la escala de colores del estilo artístico estudiado. Mediante la presentación de diferentes casos de prueba se evidencian los resultados obtenidos por el sistema antes y después de la optimización.

  2. Convertidor buck-boost controlado digitalmente con histeresis cero mediante un dsp; Back-Booost Converter digitally controlled by zero hysteresis method, using DSP

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    Fredy Edimer Hoyos Velasco

    2011-02-01

    Full Text Available En este trabajo se estudia el comportamiento de un convertidor electrónico en configuración buck-boost. Elobjetivo del controlador es hacer el rastreo de señales sinusoidales usando un controlador con histéresiscero, es decir que el usuario pueda tener a la salida cualquier señal a un nivel más alto de potencia y conla característica que esta señal deseada es regulada variable en frecuencia, en amplitud y forma de onda.El controlador es rápido pues se comprobó en el laboratorio que ante perturbaciones en la carga y en laseñal de referencia, se comporta adecuadamente. Se implementó el convertidor usando un inversormonofásico en configuración de medio puente y se controla el sistema con un prototipo rápido de control(RCP DS1104 en tiempo real. In this work, we analysed the behaviour of a buck-boost electronic converter. The objective of the zerohysteresis controller is to track user defined sinusoidal signals, usually featuring high power level andvariable frequency, amplitude and wave form. The controller was implemented in the laboratory, whereload and reference signal disturbances were introduced. The controller achieved a fast and adequatebehaviour. The converter was implemented using a half bridge monophasic inverter, and a rapid controlprototyping (RCP DS1104 in real time for the control task.

  3. Diseño óptimo de un disipador de calor para luminaria LED mediante moderación modelación computacional

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    Daniel Cahue Díaz

    2014-01-01

    Full Text Available En el presente trabajo se desarrolla una selección de materiales y simulación térmica en el diseño de disipadores de calor para sistemas de iluminación de estado sólido (SSL mejor conocidos como luminarias LEDs. Se desarrolló un modelo matemático con la capacidad de predecir el comportamiento térmico de la luminaria cuando se encuentra en operación. El modelo matemático fue resuelto mediante un software de distribución libre el cual permite resolver ecuaciones diferenciales mediante el método de elemento finito. Los resultados obtenidos en el modelo matemático planteado fueron validados con los resultados obtenidos mediante experimentación usando imágenes termográficas.

  4. Orbitotomía lateral mediante abordaje temporal Lateral orbitotomy using a temporal approach

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    H. Herencia Nieto

    2005-12-01

    Full Text Available La orbitotomía lateral sigue siendo en el momento actual la técnica quirúrgica de elección para la biopsia o extirpación de lesiones intraorbitarias laterales al nervio óptico, la biopsia del propio nervio óptico y la extirpación de la glándula lacrimal. Se han descrito múltiples incisiones cutáneas para llevar a cabo esta intervención; la más frecuentemente empleada por el momento es la incisión palpebral, que discurre a nivel de alguna arruga del párpado superior. Aunque los resultados obtenidos con esta incisión suelen ser aceptables, no está exenta de complicaciones, ni estéticas ni funcionales. Por esto, en los últimos años han ido apareciendo nuevas incisiones que intentan evitar estas complicaciones. Entre estas nuevas vías de abordaje a la pared lateral de la órbita se encuentra la incisión temporal. En los últimos 3 años, se ha intervenido en nuestro servicio a cuatro pacientes para llevar a cabo biopsia o extirpación de masas intraorbitarias mediante orbitotomía lateral, siempre a través de una incisión temporal. En todos los casos esta incisión permitió una amplia exposición del campo quirúrgico y la cómoda realización de la intervención, obteniéndose resultados estéticos y funcionales excelentes, y sin ninguna secuela ni complicación permanente. La complicación específica más frecuente de esta incisión es la paresia de la rama frontal del nervio facial. Una técnica de disección cuidadosa suele ser suficiente para evitarla. Todo esto hace que esta incisión, tal y como la describimos aquí, sea de elección para llevar a cabo la orbitotomía lateral, constituyendo en nuestro criterio una alternativa perfectamente válida y a tener en cuenta frente a las incisiones «clásicas», a las que llega a superar en muchos aspectos.The lateral orbitotomy it still the surgical technique of choice for biopsies or the removal of intraorbital lesions that are lateral to the optic nerve, for biopsies of

  5. Proyecto de instalación eléctrica para el abastecimiento de una vivienda mediante energías renovables

    OpenAIRE

    Zabaleta Miguel, Jabier

    2015-01-01

    El proyecto se centra en abastecer la demanda eléctrica del Centro Canino Berquier (Añorbe – Zona Media de Navarra), que cuenta una residencia canina, una consulta veterinaria y una vivienda, mediante un sistema aislado de la red y el uso de energías renovables. El abastecimiento de energía eléctrica mediante energías renovables, contará con un sistema de generación, almacenamiento y distribución, para garantizar un suministro en cantidad y calidad suficiente, del Centro Canino Berquier, ...

  6. Creación de una librería de elementos mecánicos rotacionales mediante EcosimPro

    OpenAIRE

    Nieva Hernando, Sergio

    2014-01-01

    El objetivo del presente proyecto es la simulación de sistemas mecánicos dinámicos rotacionales mediante la herramienta de simulación EcosimPro. Para ello, se va a crear una librería con elementos mecánicos rotacionales, la cual va a contener los componentes necesarios para formar los sistemas dinámicos a simular. Una vez creados estos elementos de forma individual, se podrán unir mediante diferentes nexos para formar sistemas más complejos de manera que el estudio se pueda tra...

  7. Diseño y construcción de un vehículo de inspección remota mediante marcación por tonos

    OpenAIRE

    Ruiz Díez, Pablo

    2009-01-01

    El principal objetivo de este trabajo consiste en diseñar y construir un vehiculo de inspección mediante telefonía móvil que permita visualizar la zona de interés en tiempo real y de forma remota poder controlar su desplazamiento utilizando las teclas de un teléfono. En primer lugar, se realizará el diseño y construcción de un vehiculo triciclo capaz de desplazarse en cualquier dirección mediante motores de continua, se tratara de un sistema de dos ruedas motrices cada una con su propio motor...

  8. Estudio comparativo del comportamiento de losas de concreto reforzado mediante los análisis elástico y límite

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    Julio Vergara García

    2011-06-01

    Full Text Available Presenta un resumen de la tesis de grado "Estudio comparativo de losas de concreto reforzado mediante los análisis elástico y límite". Este trabajo proporciona fórmulas, tablas y gráficas prácticas para determinar los momentos flectores y el volumen de refuerzo de los tipos de losas estudiados, sometidas a diferentes tipos de carga y analizados mediante la Teoría de la Elasticidad y el Análisis Límite.

  9. Cálculo del tratamiento óptimo económico de insumos agrícolas mediante un procedimiento numérico discreto

    OpenAIRE

    Ernesto Aceves-Ruiz; Antonio Turrent-Fernández; José I. Cortés-Flores; Víctor Volke-Haller

    2005-01-01

    Con el propósito de reducir los costos de la generación de recomendaciones para el manejo de los cultivos, se propone un método en el que se realiza el modelaje de los niveles del óptimo económico de N, P, K y densidad de población (Dp) en el cultivo de maíz, mediante una aproximación binomial. Por cada factor de estudio, se seleccionaron cinco factores de diagnóstico, que minimizaran la discrepancia entre los niveles de los óptimos observados y los calculados mediante un modelo aditivo. El e...

  10. Evaluación funcional de la vía visual en adultos ambliopes mediante los potenciales evocados visuales multifocales

    OpenAIRE

    García Romo, María Esperanza

    2016-01-01

    La ambliopía es una patología que asocia diversas anomalías funcionales, con una prevalencia del 1 - 3,5% que afecta a la población pediátrica y adulta. OBJETIVOS Y METODOLOGÍA: En una primera parte de la tesis, mediante un estudio clínico observacional, longitudinal y prospectivo con 24 sujetos ambliopes y 19 sujetos controles sanos, se hizo una evaluación funcional de la vía visual mediante los potenciales evocados visuales multifocales (PEVmf) y su correlación con la evaluación clínica, pr...

  11. Observador de estado de un sistema de generación de energía de tipo celda de combustible PEM mediante un modelo lineal variante

    OpenAIRE

    Lira, S. de; Puig, Vicenç; Quevedo, Joseba; Husar, Attila

    2010-01-01

    [ES]: En este trabajo se presenta el modelado de un sistema generador de energía basado en pilas de combustible PEM mediante un modelo variante con el punto de operación (modelo LPV). Dicho modelo ha sido obtenido mediante la linealización del modelo dinámico no lineal que ha sido previamente calibrado con datos reales de laboratorio. El modelo LPV obtenido está pensado para ser utilizado en el diseño de controladores y algoritmos de diagnóstico de fallos basados en modelos.

  12. Ensayo no destructivo de soldaduras en pernos conectores mediante inspección acústica = Non-destructive test of welding headed studs through acoustic emission

    OpenAIRE

    Aznar López, Antonio; Cervera Bravo, Jaime; Ortiz Herrera, Jesus Maria; Hernando Garcia, Jose Ignacio

    2012-01-01

    Los pernos conectores aportan múltiples ventajas de uso, entre las que se encuentra el elevado margen de seguridad que ofrecen sus soldaduras ejecutadas mediante arco eléctrico. Estas soldaduras, aunque ampliamente fiables, son difícilmente comprobadas mediante ensayos no destructivos. Aparte de la inspección visual, que aporta gran información sobre la calidad de ejecución de la soldadura, el resto de ensayos no destructivos (líquidos penetrantes, partículas magnéticas, ultrasonidos, radiogr...

  13. Morfología comparada de la escápula de primates humanos y no humanos mediante morfometría geométrica. Estudio preliminar.

    OpenAIRE

    2013-01-01

    Este trabajo estudia la morfología comparada de la escápula de primates humanos y no humanos mediante morfometría geométrica. Se han utilizado 62 omóplatos de individuos adultos de sexo desconocido (22 humanos actuales, 1 Neandertal y 39 primates no humanos), procedentes de las colecciones de las Universidades UAB, UB y del Museo de Ciencias Naturales de Barcelona. Cada escápula se fotografió ortogonalmente y se cuantificó su morfología mediante el uso de puntos anatómicos de referencia (12 p...

  14. Ciberacoso mediante teléfono móvil e Internet en las relaciones de noviazgo entre jóvenes

    OpenAIRE

    Roberto Martínez Pecino; Mercedes Durán Segura

    2015-01-01

    El ciberacoso es un fenómeno ampliamente analizado entre adolescentes, sin embargo en España ha sido poco estudiado entre jóvenes y particularmente en sus relaciones de noviazgo. Empleando una metodología cuantitativa este estudio analiza el ciberacoso mediante el teléfono móvil e Internet en las relaciones de noviazgo en una muestra compuesta por 336 estudiantes universitarios. El análisis de resultados indica que un 57,2% declara haber sido victimizado por su pareja mediante el teléfono móv...

  15. VALORACIÓN SOCIOECONÓMICA DE LA EXTRACCIÓN DE GAS MEDIANTE FRACTURACIÓN HIDRÁULICA EN LA REGIÓN DE MURCIA

    OpenAIRE

    José M. Martínez-Paz; Francisco Pellicer-Martínez; José Ramón Fernández; Luca Lamonaca

    2015-01-01

    La extracción de gas no convencional mediante fracturación hidráulica (fracking) es una técnica controvertida, ya que a sus beneficios sociales y económicos se contraponen sus potenciales riesgos ambientales y sobre la salud humana. En el norte de la Región de Murcia, al igual que en otras zonas de España, se han concedido recientemente permisos de investigación y prospección para la posible explotación de yacimientos de gas mediante fracking, lo que ha originado la aparición de movimientos c...

  16. Promoción del trabajo colaborativo mediante la integración de las tecnologías digitales en el contexto escolar

    OpenAIRE

    Baltodano Enríquez, Manuel

    2017-01-01

    Este ensayo presenta algunas estrategias para el desarrollo de proyectos pedagógicos mediante la promoción del trabajo colaborativo con el apoyo de las tecnologías digitales. Durante varios años, el Departamento de Investigación, Desarrollo e Implementación de la Dirección de Recursos Tecnológicos en Educación del Ministerio de Educación Pública de Costa Rica, ha promovido diversas iniciativas para potenciar ambientes de aprendizaje colaborativo entre educadores y estudiantes, mediante el apr...

  17. Diferencias en la calciuria, estimada mediante el índice Ca/Cr en función del tipo de lactancia

    OpenAIRE

    Trigo López, Javier

    2015-01-01

    Se ha realizado un estudio descriptivo transversal sobre el comportamiento de la calciuria, estimada mediante el índice calcio/creatinina (ICC), en lactantes menores de 6 meses, analizando su posible relación con el tipo de alimentación (leche de fórmula o leche materna). Los resultados se obtuvieron a partir de muestras de orina de 44 lactantes sanos en los que se recoge el tipo de lactancia. El grupo alimentado mediante leche de fórmula presentó un ICC medio expresado en mg/mg de 0,59, mien...

  18. Automatización mediante PC de los procesos de calibración de bloques patrón longitudinales según la norma ISO 3650

    OpenAIRE

    2009-01-01

    Este proyecto nace a través de la necesidad del laboratorio de metrología del Centro Tecnológico de Vilanova y la Geltrú de automatizar el proceso de calibración por comparación mecánica de bloques patrones longitudinales (BPL) el cual se realiza totalmente de forma manual. El objetivo es implementar una aplicación, mediante el software de programación gráfica Labview, que se comunique con la máquina de calibración TESA upc mediante comunicación serie RS232y conseguir ...

  19. Vida a la fatiga de juntas soldadas del acero inoxidable AISI 316L obtenidas mediante el proceso GMAW

    Directory of Open Access Journals (Sweden)

    Puchi-Cabrera, E. S.

    2007-06-01

    Full Text Available An investigation has been conducted in order to determine the effect of both the metallic transfer mode (pulsed arc or short circuit and the O2 content in the Ar/O2 gas mixture, of the gas-metal arc welding process (GMAW, on the fatigue life under uniaxial conditions of welded joints of 316L stainless steel. It has been concluded that the mixture of the protective gases employed in the process could have an important influence on the fatigue life of the welded joints of such steel in two different ways. Firstly, through the modification of the radius of curvature at the joint between the welding toe and the base metal and, secondly, through a more pronounced degree of oxidation of the alloying elements induced by a higher O2 content in the mixture. As far as the metallic transfer mode is concerned, it has been determined that the welded joints obtained under a pulsed arc mode show a greater fatigue life in comparison with the joints obtained under short circuit for both gas mixtures.

    Se ha llevado a cabo una investigación con la finalidad de determinar el efecto, tanto del modo de transferencia metálica (arco pulsado o cortocircuito como del contenido de O2 en la mezcla de gases protectores Ar/O2, del proceso de soldadura a tope mediante arco metálico con protección gaseosa (GMAW, sobre la vida a la fatiga en condiciones uniaxiales de juntas soldadas del acero inoxidable AISI 316L. Dicho trabajo ha permitido concluir que la composición de la mezcla de gases protectores del proceso GMAW pudiera tener una influencia importante en la vida a la fatiga de las juntas soldadas de dicho material, a través de dos formas distintas: primero, mediante la modificación del radio de curvatura entre la raíz del cordón de soldadura y el metal base y, en segundo lugar, a través del mayor grado de oxidación de los elementos de aleación. En cuanto al modo de transferencia metálica, se determinó que las juntas soldadas mediante arco pulsado

  20. Obtención de extractos de membrana externa de Vibrio cholerae O1, mediante el uso de diferentes detergentes

    Directory of Open Access Journals (Sweden)

    José Luis Pérez

    2006-04-01

    Full Text Available En la actualidad existen dos variantes principales de vacunas orales contra el cólera: una basada en células inactivadas de diferentes biotipos y serotipos y otra basada en la administración de cepas vivas genéticamente atenuadas. Una vacuna por subunidades pudiera ser una variante muy atractiva. Este trabajo describe la purificación parcial y caracterización preliminar de extractos de proteínas de membrana externa-lipopolisacárido (PME-LPS, obtenidos a partir de Vibrio cholerae O1, con el interés de seleccionar un proteoliposoma que posteriormente será estructurado en forma de cocleatos para su uso por vía oral en humanos. Las preparaciones fueron obtenidas a través del uso de diferentes detergentes. La cantidad de LPS en cada preparación fue estimada mediante la determinación de las unidades endotóxicas en el ensayo del Limulus (LAL. La composición de cada muestra fue evaluada mediante SDS-PAGE y Dot Blot. La inoculación intranasal (IN en ratones Balb/c se utilizó para la evaluación de la inmunogenicidad de las preparaciones, y la respuesta inmune fue determinada por ELISA y el título de anticuerpos vibriocidas. El tamaño molecular de la preparación con mejores resultados en inmunogenicidad se estimó mediante la cromatografía en Sephacryl S-1000. Se obtuvieron diferentes perfiles electroforéticos de acuerdo con el tipo de detergente utilizado. El LPS fue identificado en todas las preparaciones y aquella obtenida con el SDS al 15% mostró la más baja relación proteínas/LPS y los mejores resultados en los ensayos de inmunogenicidad. Adicionalmente se comprobó que su tamaño molecular es similar al observado en el proteoliposoma de VAMENGOC- BC. La preparación obtenida con el SDS al 15% constituye un proteoliposoma, con capacidad para estimular altos niveles de anticuerpos IgG anti-LPS y altos títulos de anticuerpos vibriocidas, luego de su administración por vía intranasal en ratones. Estos resultados constituyen