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Sample records for hydrilla verticillata lf

  1. Phytochelatins and antioxidant systems respond differentially during arsenite and arsenate stress in Hydrilla verticillata (L.f.) Royle.

    Science.gov (United States)

    Srivastava, S; Mishra, S; Tripathi, R D; Dwivedi, S; Trivedi, P K; Tandon, P K

    2007-04-15

    Serious contamination of aquatic systems by arsenic (As) in different parts of the world calls for the development of an in situ cost-effective phytoremediation technology. In the present investigation, plants of Hydrilla verticillata (L.f.) Royle were exposed to various concentrations of arsenate (As(V)) (0-250 microM) and arsenite (AsIII) (0-25 microM) and analyzed for accumulation responses vis-à-vis biochemical changes. Total As accumulation was found to be higher in plants exposed to AsIII (315 microg g(-1) dw at 25 microM) compared to As(V) (205 microg g(-1) dw at 250 microM) after 7 d of treatment. Plants tolerated low concentrations of As(III) and As(V) by detoxifying the metalloid through augmented synthesis of thiols such as phytochelatins and through increased activity of antioxidant enzymes. While As(V) predominantly stimulated antioxidant enzyme activity, As(III) primarily caused enhanced levels of thiols. The maximum amount of As chelated by PCs was found to be about 39% in plants exposed to As(III) (at 10 microM) and 35% in As(V) exposed plants (at 50 microM) after 4 d. Only the respective highest concentrations of As(III) (25 microM) and As(V) (250 microM) proved toxic for normal plant growth after prolonged treatment. Thus, H. verticillata forms a promising candidate for the phytoremediation of As contaminated water.

  2. Copper-induced oxidative stress and responses of antioxidants and phytochelatins in Hydrilla verticillata (L.f.) Royle.

    Science.gov (United States)

    Srivastava, Sudhakar; Mishra, Seema; Tripathi, Rudra D; Dwivedi, Sanjay; Gupta, Dharmendra K

    2006-12-30

    Copper, though essential, is potentially toxic heavy metal at supraoptimal level and has widespread contamination. The present investigation was carried out to study the responses induced by lower as well as higher doses of copper (0.1-25 microM) in an aquatic macrophyte, Hydrilla verticillata (L.f.) Royle for a period of 1-7 days. The plants accumulated copper in high amount with a maximum of 770 microg g(-1) dw on day 7 at 25 microM. Biomass and photosynthetic pigments showed less alteration up to 1 microM while at higher concentrations, significant decline occurred. Malondialdehyde (MDA) content and electrical conductivity (EC) also showed sharp increase at higher concentrations indicating oxidative stress. In response to copper exposure, plants showed significant induction of proteins and enzymes like superoxide dismutase (SOD), ascorbate peroxidase (APX), guaiacol peroxidase (GPX), catalase (CAT) and glutathione reductase (GR), however, only up to moderate exposures. Total non-protein thiols (NP-SH) and cysteine levels increased significantly up to 5 microM copper exposure while at 25 microM, their level declined drastically. Reduced glutathione (GSH) showed decrease at all concentrations while oxidized glutathione (GSSG) simultaneously increased. Phytochelatins (PCs) were also induced significantly at studied concentrations of 1 and 5 microM on day 4 in comparison to control. However, copper chelation depicted by PC-SH to copper ratio was found to be low (6.5% at 1 microM and 2.4% at 5 microM) suggesting that PCs play only a part in integrated mechanisms of copper homeostasis and detoxification. Tolerant response of plants to moderate copper exposures and high accumulation potential warrants their suitability for remediation of moderately copper polluted water bodies.

  3. Induction of phytochelatins in hydrilla verticillata (l.f.) Royle under cadmium stress

    Energy Technology Data Exchange (ETDEWEB)

    Tripathi, R.D.; Rai, U.N.; Gupta, M. [National Botanical Research Institute, Lucknow (India)] [and others

    1996-03-01

    Plants tolerate Cd by sequestering them through synthesizing phytochelatins with the general structure (t-Glu-cys)n-gly where n= 2-11 depending upon the species from which these peptides are isolated. Recent biochemical evidence suggests that these peptides are synthesized via posttranslationally activated, metal-dependent enzymatic pathways from the precursor glutathione. However, most of these studies are confined to terrestrial species and only a few studies have been made on higher aquatic plants. Recently H. verticillata and other aquatic higher plants have been reported to be hyperaccumulators of Cd and have demonstrated the ability to remove many toxic metals, including Cd, from wastewater. It is hypothesized that cadmium hyperaccumulating ability of the macrophyte is associated with induction of the metal chelating peptides, the phytochelatins (PCs), to copeup with high cellular Cd levels. In view of this, it was considered worthwhile to examine the induction of phytochelatins and changes in levels of glutathione and related metabolites in H. verticillata under Cd stress.

  4. Evaluation of uranium removal by Hydrilla verticillata (L.f.) Royle from low level nuclear waste under laboratory conditions.

    Science.gov (United States)

    Srivastava, Sudhakar; Bhainsa, K C

    2016-02-01

    The present study evaluated uranium (U) removal ability and tolerance to low level nuclear waste (LLNW) of an aquatic weed Hydrilla verticillata. Plants were screened for growth in 10%-50% waste treatments up to 3 d. Treatments of 20% and 50% waste imposed increasing toxicity with duration assessed in terms of change in fresh weight and in the levels of photosynthetic pigments and thiobarbituric acid-reactive substances. U concentration, however, did not show a progressive increase and was about 42 μg g(-1) dw from 20% to 50% waste at 3 d. This suggested that a saturation stage was reached with respect to U removal due to increasing toxicity. However, in another experiment with 10% waste and 10% waste+10 ppm U treatments, plants showed an increase in U concentration with the maximum level approaching 426 μg g(-1) dw at 3 d without showing any toxicity as compared to that at 20% and 50% waste treatments. Hence, plants possessed significant potential to take up U and toxicity of LLNW limited their U removal ability. This implies that the use of Hydrilla plants for U removal from LLNW is feasible at low concentrations and would require repeated harvesting at short intervals.

  5. Integrated role of ROS and Ca(+2) in blue light-induced chloroplast avoidance movement in leaves of Hydrilla verticillata (L.f.) Royle.

    Science.gov (United States)

    Majumdar, Arkajo; Kar, Rup Kumar

    2016-11-01

    Directional chloroplast photorelocation is a major physio-biochemical mechanism that allows these organelles to realign themselves intracellularly in response to the intensity of the incident light as an adaptive response. Signaling processes involved in blue light (BL)-dependent chloroplast movements were investigated in Hydrilla verticillata (L.f.) Royle leaves. Treatments with antagonists of actin filaments [2,3,5-triiodobenzoic acid (TIBA)] and microtubules (oryzalin) revealed that actin filaments, but not microtubules, play a pivotal role in chloroplast movement. Involvement of reactive oxygen species (ROS) in controlling chloroplast avoidance movement has been demonstrated, as exogenous H2O2 not only accelerated chloroplast avoidance but also could induce chloroplast avoidance even in weak blue light (WBL). Further support came from experiments with different ROS scavengers, i.e., dimethylthiourea (DMTU), KI, and CuCl2, which inhibited chloroplast avoidance, and from ROS localization using specific stains. Such avoidance was also partially inhibited by ZnCl2, an inhibitor of NADPH oxidase (NOX) as well as 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), a photosynthetic electron transport chain (ETC) inhibitor at PS II. However, methyl viologen (MV), a PS I ETC inhibitor, rather accelerated avoidance response. Exogenous calcium (Ca(+2)) induced avoidance even in WBL while inhibited chloroplast accumulation partially. On the other hand, chloroplast movements (both accumulation and avoidance) were blocked by Ca(+2) antagonists, La(3+) (inhibitor of plasma membrane Ca(+2) channel) and ethylene glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA, Ca(+2) chelator) while LiCl that affects Ca(+2) release from endosomal compartments did not show any effect. A model on integrated role of ROS and Ca(+2) (influx from apolastic space) in actin-mediated chloroplast avoidance has been proposed.

  6. Captive bubble and sessile drop surface characterization of a submerged aquatic plant, Hydrilla verticillata

    Science.gov (United States)

    The surface energy parameters of the invasive aquatic weed, Hydrilla verticillata, were determined using contact angle measurements using two different methods. The abaxial and adaxial surfaces of the leaves and stem were characterized for the weed while submerged in water using captive air and octa...

  7. Life cycle assessment of nutrient remediation and bioenergy production potential from the harvest of hydrilla (Hydrilla verticillata).

    Science.gov (United States)

    Evans, Jason M; Wilkie, Ann C

    2010-12-01

    Hydrilla (Hydrilla verticillata) is one of the world's most problematic invasive aquatic plants. Although management of hydrilla overgrowth has often been based on use of chemical herbicides, issues such as the emergence of herbicide-resistant hydrilla biotypes and the need for in situ nutrient remediation strategies have together raised interest in the use of harvester machines as an alternative management approach. Using a life cycle assessment (LCA) approach, we calculated a range of net energy and economic benefits associated with hydrilla harvests and the utilization of biomass for biogas and compost production. Base case scenarios that used moderate data assumptions showed net energy benefit ratios (NEBRs) of 1.54 for biogas production and 1.32 for compost production pathways. NEBRs for these respective pathways rose to 2.11 and 2.68 when labor was excluded as a fossil fuel input. Base case biogas and compost production scenarios respectively showed a monetary benefit cost ratio (BCR) of 1.79 and 1.83. Moreover, very high NEBRs (3.94 for biogas; 6.37 for compost) and BCRs (>11 for both biogas and compost) were found for optimistic scenarios in which waterways were assumed to have high hydrilla biomass density, high nutrient content in biomass, and high priority for nutrient remediation. Energy and economic returns were largely decoupled, with biogas and fertilizer providing the bulk of output energy, while nutrient remediation and herbicide avoidance dominated the economic output calculations. Based on these results, we conclude that hydrilla harvest is likely a suitable and cost-effective management program for many nutrient-impaired waters. Additional research is needed to determine how hydrilla harvesting programs may be most effectively implemented in conjunction with fish and wildlife enhancement objectives.

  8. An extract of Hydrilla verticillata and associated epiphytes induces avian vacuolar myelinopathy in laboratory mallards.

    Science.gov (United States)

    Wiley, Faith E; Twiner, Michael J; Leighfield, Tod A; Wilde, Susan B; Van Dolah, Frances M; Fischer, John R; Bowerman, William W

    2009-08-01

    Avian vacuolar myelinopathy (AVM) is a neurological disease affecting bald eagles (Haliaeetus leucocephalus), American coots (Fulica americana), waterfowl, and other birds in the southeastern United States. The cause of the disease is unknown, but is thought to be a naturally produced toxin. AVM is associated with aquatic macrophytes, most frequently hydrilla (Hydrilla verticillata), and researchers have linked the disease to an epiphytic cyanobacterial species associated with the macrophytes. The goal of this study was to develop an extraction protocol for separating the putative toxin from a hydrilla-cyanobacterial matrix. Hydrilla samples were collected from an AVM-affected reservoir (J. Strom Thurmond Lake, SC) and confirmed to contain the etiologic agent by mallard (Anas platyrhynchos) bioassay. These samples were then extracted using a solvent series of increasing polarity: hexanes, acetone, and methanol. Control hydrilla samples from a reference reservoir with no history of AVM (Lake Marion, SC) were extracted in parallel. Resulting extracts were administered to mallards by oral gavage. Our findings indicate that the methanol extracts of hydrilla collected from the AVM-affected site induced the disease in laboratory mallards. This study provides the first data documenting for an "extractable" AVM-inducing agent.

  9. La descontaminación de las aguas del lago Izabal en Guatemala a través de la extracción de la planta Hydrilla verticillata (L.F Royle y su uso como sustrato alternativo para la producción de plántulas de chile pimiento en invernadero

    Directory of Open Access Journals (Sweden)

    Iván Dimitri Santos Castillo

    2010-01-01

    Full Text Available Se caracterizaron las principales propiedades físicas, químicas y biológicas de sustratos elaborados a base de residuos de hydrilla verticillata y su efecto sobre la germinación y desarrollo durante la fase de plántula de semillas de chile pimiento (variedad tropical irazú mejorado. Al analizar los resultados obtenidos tales como agua fácilmente aprovechable, capacidad de aereación, PH, porciento de germinación, germinación acumulada ( 24 DDS, altura de la planta (cm, diámetro del tallo (cm, peso fresco de la parte aérea y sistema radical (g, materia seca de la parte aérea y radical ( % , todo ello 50 días después de siembra (50 DDS, se obtiene que el mejor tratamiento bajo las condiciones y metodologías utilizadas es el N0 12 (H30-85-15, o sea, 85% de hydrilla con 30 días de compostaje + 15 % de perlita y se recomienda como sustituto alternativo del sustrato comercial (turba de sphagnum para la producción de plántulas de chile pimiento en pilones y con ello dar uso productivo a esta planta contaminante de los lagos de Guatemala.

  10. Interspecific competition effects on phosphorus accumulation by Hydrilla verticillata and Vallisneria natans

    Institute of Scientific and Technical Information of China (English)

    Xiufeng Zhang; Zhengwen Liu

    2011-01-01

    The competition between submersed plants has been recognized as an important factor influencing the structure of plant communities in shallow lakes.The ability of different species to take up and store nutrients from the surrounding ambience varies,and hence plant community structure might be expected to affect the cycling of nutrients in lake ecosystems.In this study,the uptake of phosphorus by Hydrilla verticillata and Vallisneria natans was studied and compared in monoculture and competitive mixed-culture plantings.Results showed that for both studied species the phosphorus concentrations of different tissues and of whole plants was unaffected by competition.However,the quantity of phosphorus accumulated by whole plants of H.verticillata was significantly higher in mixture culture than in monoculture,while that of V.natans was lower in the mixed culture.The results indicated that H.verticillata has a competitive advantage over V.natant,when the two species are grown in competition,and is able to accumulate a greater quantity of phosphorus.

  11. Experimental feeding of Hydrilla verticillata colonized by stigonematales cyanobacteria induces vacuolar myelinopathy in painted turtles (Chrysemys picta.

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    Albert D Mercurio

    Full Text Available Vacuolar myelinopathy (VM is a neurologic disease primarily found in birds that occurs when wildlife ingest submerged aquatic vegetation colonized by an uncharacterized toxin-producing cyanobacterium (hereafter "UCB" for "uncharacterized cyanobacterium". Turtles are among the closest extant relatives of birds and many species directly and/or indirectly consume aquatic vegetation. However, it is unknown whether turtles can develop VM. We conducted a feeding trial to determine whether painted turtles (Chrysemys picta would develop VM after feeding on Hydrilla (Hydrilla verticillata, colonized by the UCB (Hydrilla is the most common "host" of UCB. We hypothesized turtles fed Hydrilla colonized by the UCB would exhibit neurologic impairment and vacuolation of nervous tissues, whereas turtles fed Hydrilla free of the UCB would not. The ability of Hydrilla colonized by the UCB to cause VM (hereafter, "toxicity" was verified by feeding it to domestic chickens (Gallus gallus domesticus or necropsy of field collected American coots (Fulica americana captured at the site of Hydrilla collections. We randomly assigned ten wild-caught turtles into toxic or non-toxic Hydrilla feeding groups and delivered the diets for up to 97 days. Between days 82 and 89, all turtles fed toxic Hydrilla displayed physical and/or neurologic impairment. Histologic examination of the brain and spinal cord revealed vacuolations in all treatment turtles. None of the control turtles exhibited neurologic impairment or had detectable brain or spinal cord vacuolations. This is the first evidence that freshwater turtles can become neurologically impaired and develop vacuolations after consuming toxic Hydrilla colonized with the UCB. The southeastern United States, where outbreaks of VM occur regularly and where vegetation colonized by the UCB is common, is also a global hotspot of freshwater turtle diversity. Our results suggest that further investigations into the effect of the

  12. Experimental feeding of Hydrilla verticillata colonized by stigonematales cyanobacteria induces vacuolar myelinopathy in painted turtles (Chrysemys picta).

    Science.gov (United States)

    Mercurio, Albert D; Hernandez, Sonia M; Maerz, John C; Yabsley, Michael J; Ellis, Angela E; Coleman, Amanda L; Shelnutt, Leslie M; Fischer, John R; Wilde, Susan B

    2014-01-01

    Vacuolar myelinopathy (VM) is a neurologic disease primarily found in birds that occurs when wildlife ingest submerged aquatic vegetation colonized by an uncharacterized toxin-producing cyanobacterium (hereafter "UCB" for "uncharacterized cyanobacterium"). Turtles are among the closest extant relatives of birds and many species directly and/or indirectly consume aquatic vegetation. However, it is unknown whether turtles can develop VM. We conducted a feeding trial to determine whether painted turtles (Chrysemys picta) would develop VM after feeding on Hydrilla (Hydrilla verticillata), colonized by the UCB (Hydrilla is the most common "host" of UCB). We hypothesized turtles fed Hydrilla colonized by the UCB would exhibit neurologic impairment and vacuolation of nervous tissues, whereas turtles fed Hydrilla free of the UCB would not. The ability of Hydrilla colonized by the UCB to cause VM (hereafter, "toxicity") was verified by feeding it to domestic chickens (Gallus gallus domesticus) or necropsy of field collected American coots (Fulica americana) captured at the site of Hydrilla collections. We randomly assigned ten wild-caught turtles into toxic or non-toxic Hydrilla feeding groups and delivered the diets for up to 97 days. Between days 82 and 89, all turtles fed toxic Hydrilla displayed physical and/or neurologic impairment. Histologic examination of the brain and spinal cord revealed vacuolations in all treatment turtles. None of the control turtles exhibited neurologic impairment or had detectable brain or spinal cord vacuolations. This is the first evidence that freshwater turtles can become neurologically impaired and develop vacuolations after consuming toxic Hydrilla colonized with the UCB. The southeastern United States, where outbreaks of VM occur regularly and where vegetation colonized by the UCB is common, is also a global hotspot of freshwater turtle diversity. Our results suggest that further investigations into the effect of the putative UCB toxin

  13. A CO2-flux mechanism operating via pH-polarity in Hydrilla verticillata leaves with C-3 and C-4 photosynthesis

    NARCIS (Netherlands)

    van Ginkel, LC; Bowes, G; Reiskind, JB; Prins, HBA

    2001-01-01

    The aquatic angiosperm Hydrilla verticillata lacks Kranz anatomy, but has an inducible, C-4-based, CO2 concentrating mechanism (CCM) that concentrates CO2 in the chloroplasts. Both C-3 and C-4 Hydrilla leaves showed light-dependent pH polarity that was suppressed by high dissolved inorganic carbon (

  14. A social analysis of the bioinvasions of Dreissena polymorpha in Spain and Hydrilla verticillata in Guatemala.

    Science.gov (United States)

    Binimelis, Rosa; Monterroso, Iliana; Rodríguez-Labajos, Beatriz

    2007-10-01

    Human agency plays a key role in the processes of biological invasions. This comprises not only the human role in the configuration of driving forces or in the perception of the impacts, but also the conceptualization of alien species themselves as an environmental problem. This paper examines different stakeholders' positions in bioinvasion processes at different scales, and it looks at their relevance for the management of invasive species. It compares two cases: the invasion process of Dreissena polymorpha in the Ebro River in Spain and the case of Hydrilla verticillata in Lake Izabal, Guatemala. Our results are structured according to impacts and to management options. The discussion focuses on the relevance of incorporating the different stakeholders' interests and values in the analysis and management of biological invasions. Although social analysis of stakeholders' positions is necessary in order to foster management actions, it also reveals conflicts on the relevant criteria and on the very definition of invasive species.

  15. A Social Analysis of the Bioinvasions of Dreissena polymorpha in Spain and Hydrilla verticillata in Guatemala

    Science.gov (United States)

    Binimelis, Rosa; Monterroso, Iliana; Rodríguez-Labajos, Beatriz

    2007-10-01

    Human agency plays a key role in the processes of biological invasions. This comprises not only the human role in the configuration of driving forces or in the perception of the impacts, but also the conceptualization of alien species themselves as an environmental problem. This paper examines different stakeholders’ positions in bioinvasion processes at different scales, and it looks at their relevance for the management of invasive species. It compares two cases: the invasion process of Dreissena polymorpha in the Ebro River in Spain and the case of Hydrilla verticillata in Lake Izabal, Guatemala. Our results are structured according to impacts and to management options. The discussion focuses on the relevance of incorporating the different stakeholders’ interests and values in the analysis and management of biological invasions. Although social analysis of stakeholders’ positions is necessary in order to foster management actions, it also reveals conflicts on the relevant criteria and on the very definition of invasive species.

  16. Effects of chitosan on growth of an aquatic plant (Hydrilla verticillata) in polluted waters with different chemical oxygen demands

    Institute of Scientific and Technical Information of China (English)

    XU Qiu-jin; NIAN Yue-gang; JIN Xiang-can; YAN Chang-zhou; LIU Jin; Jiang Gao-ming

    2007-01-01

    Effects of chitosan on a submersed plant, Hydrilla verticillata, were investigated. Results indicated that H. verticillata could prevent ultrastructure phytotoxicities and oxidativereaction from polluted water with high chemical oxygen demand (COD). Superoxide dismutase (SOD) activity and malondialdehyde (MDA) contents in H. verticillata treated with 0.1% chitosan in wastewater increased with high COD (980 mg/L) and decreased with low COD (63 mg/L), respectively. Ultrastructural analysis showed that the stroma and grana of chloroplast basically remained normal. However, plant cells from the control experiment (untreated with chitosan) were vacuolated and the cell interval increased. The relict of protoplast moved to the center, with cells tending to disjoint. Our findings indicate that wastewater with high COD concentration can cause a substantial damage to submersed plant, nevertheless, chitosan probably could alleviate the membrane lipid peroxidization and ultrastructure phytotoxicities, and protect plant cells from stress of high COD concentration polluted water.

  17. Redox state and energetic equilibrium determine the magnitude of stress in Hydrilla verticillata upon exposure to arsenate.

    Science.gov (United States)

    Srivastava, Sudhakar; Suprasanna, Penna; D'Souza, Stanislaus Francis

    2011-10-01

    Arsenic (As) is a potential hazard to plants' health, however the mechanisms of its toxicity are yet to be properly understood. To determine the impact of redox state and energetic in stress imposition, plants of Hydrilla verticillata (L.f.) Royle, which are known to be potential accumulator of As, were exposed to 100 and 500 μM arsenate (AsV) for 4 to 96 h. Plants demonstrated significant As accumulation with the maximum being at 500 μM after 96 h (568 μg g(-1) dry weight, dw). The accumulation of As led to a significant increase in the level of reactive oxygen species, nitric oxide, carbonyl, malondialdehyde, and percentage of DNA degradation. In addition, the activity of pro-oxidant enzymes like NADPH oxidase and ascorbate oxidase also showed significant increases. These parameters collectively indicated oxidative stress, which in turn caused an increase in percentage of cell death. These negative effects were seemingly linked to an altered energetic and redox equilibrium [analyzed in terms of ATP/ADP, NADH/NAD, NADPH/NADP, reduced glutathione/oxidized glutathione, and ascorbate/dehydroascobate ratios]. Although there was significant increase in the levels of phytochelatins, the As chelating ligands, a large amount of As was presumably present as free ion particularly at 500 μM AsV, which supposedly produced toxic responses. In conclusion, the study demonstrated that the magnitude of disturbance to redox and energetic equilibrium of plants upon AsV exposure determines the extent of toxicity to plants.

  18. Application of ADM1 for modeling of biogas production from anaerobic digestion of Hydrilla verticillata.

    Science.gov (United States)

    Chen, Xiaojuan; Chen, Zhihua; Wang, Xun; Huo, Chan; Hu, Zhiquan; Xiao, Bo; Hu, Mian

    2016-07-01

    The present study focused on the application of anaerobic digestion model no. 1 (ADM1) to simulate biogas production from Hydrilla verticillata. Model simulation was carried out by implementing ADM1 in AQUASIM 2.0 software. Sensitivity analysis was used to select the most sensitive parameters for estimation using the absolute-relative sensitivity function. Among all the kinetic parameters, disintegration constant (kdis), hydrolysis constant of protein (khyd_pr), Monod maximum specific substrate uptake rate (km_aa, km_ac, km_h2) and half-saturation constants (Ks_aa, Ks_ac) affect biogas production significantly, which were optimized by fitting of the model equations to the data obtained from batch experiments. The ADM1 model after parameter estimation was able to well predict the experimental results of daily biogas production and biogas composition. The simulation results of evolution of organic acids, bacteria concentrations and inhibition effects also helped to get insight into the reaction mechanisms. Copyright © 2016. Published by Elsevier Ltd.

  19. New methods for the analysis of invasion processes: multi-criteria evaluation of the invasion of Hydrilla verticillata in Guatemala.

    Science.gov (United States)

    Monterroso, I; Binimelis, R; Rodríguez-Labajos, B

    2011-03-01

    The study described in this article incorporates stakeholders' views on aquatic invasion processes and combines expert analysis with information from field work into an evaluation exercise. Management scenarios are designed based on available technical data and stakeholders' perceptions. These scenarios are evaluated using the Social Multi-Criteria Evaluation framework employing the NAIADE model. Two evaluations are carried out, technical and social. Social acceptance of different management scenarios, distribution of costs and benefits, and attribution of responsibility are discussed. The case study was carried out on Lake Izabal, a body of water connected to the Caribbean Sea in Northeastern Guatemala. In 2000, local fishermen reported the presence of an alien species in the lake, the macrophyte Hydrilla verticillata. Two years later, this alien species was established around the entire lakeshore, damaging the ecosystem, endangering native species and the subsistence of local inhabitants through impacts on transportation, fishing practices, and tourism.

  20. The Ontogeny of Hydrilla verticillata(L.f.)Royle%黑藻早期个体发育的研究

    Institute of Scientific and Technical Information of China (English)

    刘玫; 王臣; 刘鸣远

    2007-01-01

    对黑藻(Hydrilla verticillata)个体发育中的胚胎发育(从合子到种子胚)和种苗发育(从种子到种苗)进行了研究.发现沉水植物黑藻与挺水植物泽泻(Alisma orientale)在胚柄只有1~2个细胞、种子胚苗端发达根端未分化、萌发后根端始分化、分生区之上产生根环与下胚轴毛、初生根短命等特点上基本相同,并对这些特点进行了讨论.

  1. Evaluation of peroxidase as a biochemical indicator of toxic chemical exposure in the aquatic plant Hydrilla verticillata, Royle

    Science.gov (United States)

    Byl, T.D.; Sutton, H.D.; Klaine, S.J.

    1994-01-01

    Laboratory bioassays were conducted to determine the utility of peroxidase (POD) activity as a biochemical indicator of contaminant exposure in the aquatic plant Hydrilla verticillata, Royle. The plants were exposed to anthracene, sulfometuron methyl (Oust??), Cd2+, Cr6+, Cu2+, Mn2+, and Se4+ in concentration factors of 10. POD was extracted and measured by spectrophotometric assay. There was a significant increase in POD activity after a 5-d exposure to each of the chemicals at 1 mg/L. The optimum pH for POD activity after exposure to the chemicals was 5.5 to 6.0. The increase in POD was found to be dose dependent for each of the chemicals. The lowest concentration of chemical to induce a significant POD increase was 0.01 mg/L for anthracene, Oust, Cd, Cr, and Cu; 0.1 mg/L for Se; and 1.0 mg/L for Mn.Laboratory bioassays were conducted to determine the utility of peroxidase (POD) activity as a biochemical indicator of contaminant exposure in the aquatic plant Hydrilla verticillata, Royle. The plants were exposed to anthracene, sulfometuron methyl (Oust), Cd2+, Cr6+, Cu2+, Mn2+, and Se4+ in concentration factors of 10. POD was extracted and measured by spectrophotometric assay. There was a significant increase in POD activity after a 5-d exposure to each of the chemicals at 1 mg/L. The optimum pH for POD activity after exposure to the chemicals was 5.5 to 6.0. The increase in POD was found to be dose dependent for each of the chemicals. The lowest concentration of chemical to induce a significant POD increase was 0.01 mg/L for anthracene, Oust, Cd, Cr, and Cu: 0.1 mg/L for Se; and 1.0 mg/L for Mn.

  2. Mass-Rearing Hydrellia Pakistanae Deonier and H. balciunasi Bock for the Management of Hydrilla verticillata

    Science.gov (United States)

    2009-06-01

    provided on rearing methods, releases, and associated costs. BACKGROUND: Hydrilla, family Hydrocharitaceae, is an exotic submersed macrophyte that...amount of topped-out, contiguous hydrilla was noted, as was the location within the reservoir (cove, boat lane, etc.). Sites containing large...as Berlese extraction with the same methods previously mentioned to monitor rearing pond numbers. Generally, two or three sites per reservoir were

  3. The phylogeographic structure of Hydrilla verticillata (Hydrocharitaceae) in China and its implications for the biogeographic history of this worldwide-distributed submerged macrophyte.

    Science.gov (United States)

    Zhu, Jinning; Yu, Dan; Xu, Xinwei

    2015-05-24

    Aquatic vascular plants are a distinctive group, differing from terrestrial plants in their growth forms and habitats. Among the various aquatic plant life forms, the evolutionary processes of freshwater submerged species are most likely distinct due to their exclusive occurrence in the discrete and patchy aquatic habitats. Using the chloroplast trnL-F region sequence data, we investigated the phylogeographic structure of a submerged macrophyte, Hydrilla verticillata, the single species in the genus Hydrilla, throughout China, in addition to combined sample data from other countries to reveal the colonisation and diversification processes of this species throughout the world. We sequenced 681 individuals from 123 sampling locations throughout China and identified a significant phylogeographic structure (NST > GST, p China indicates that China is most likely the centre of Hydrilla genetic diversity. The worldwide distribution of Hydrilla is due to recent vicariance and dispersal events that occurred in different clades during the Pleistocene. Our findings also provide useful information for the management of invasive Hydrilla in North America.

  4. Biological Control Agents of Hydrilla Verticillata; Final Report on Surveys in East Africa, 1981-1984.

    Science.gov (United States)

    1986-07-01

    eastern arm of the Rift Valley are mainly "soda" lakes (Magadi, Elmenteita, Nakuru, and Bogoria ), which are too saline to support aquatic macrophytes...J.A Lakes Magadi, Elmenteita, Nakuru, Bogoria , Turkana - no hydrilla Soda lakes of eastern Rift. First four too saline to support any submerged...inued). distribution in Uganda ( Lake Kioga), Rwanda ( Lake Bulera and Mukungwa River), and Burundi ( Lake Tanganyika). In Kenya, despite an intensive

  5. Field Surveys to Identify Biocontrol Agents of Hydrilla verticillata from June - September 2012

    Science.gov (United States)

    2015-07-01

    recreational uses, out-competes native vegetation, acts as a breeding ground for mosquitoes , and destroys fish and wildlife habitats. Hydrilla is...1 Invasion Biology and Biocontrol Lab, Wuhan Botanical Institute, CAS ERDC/TN APCRP-BC-36 July 2015 3 named previously as Bagous sp. 3 and...Continued exploration will be conducted in Guangxi Province to find new agents. ERDC/TN APCRP-BC-36 July 2015 9 Rearing, biology , and host

  6. Packed-bed column biosorption of chromium(VI) and nickel(II) onto Fenton modified Hydrilla verticillata dried biomass.

    Science.gov (United States)

    Mishra, Ashutosh; Tripathi, Brahma Dutt; Rai, Ashwani Kumar

    2016-10-01

    The present study represents the first attempt to investigate the biosorption potential of Fenton modified Hydrilla verticillata dried biomass (FMB) in removing chromium(VI) and nickel(II) ions from wastewater using up-flow packed-bed column reactor. Effects of different packed-bed column parameters such as bed height, flow rate, influent metal ion concentration and particle size were examined. The outcome of the column experiments illustrated that highest bed height (25cm); lowest flow rate (10mLmin(-1)), lowest influent metal concentration (5mgL(-1)) and smallest particle size range (0.25-0.50mm) are favourable for biosorption. The maximum biosorption capacity of FMB for chromium(VI) and nickel(II) removal were estimated to be 89.32 and 87.18mgg(-1) respectively. The breakthrough curves were analyzed using Bed Depth Service Time (BDST) and Thomas models. The experimental results obtained agree to both the models. Column regeneration experiments were also carried out using 0.1M HNO3. Results revealed good reusability of FMB during ten cycles of sorption and desorption. Performance of FMB-packed column in treating secondary effluent was also tested under identical experimental conditions. Results demonstrated significant reduction in chromium(VI) and nickel(II) ions concentration after the biosorption process.

  7. Removal of Organochlorine Pesticides by Hydrilla verticillata%黑藻对有机氯农药去除特性研究

    Institute of Scientific and Technical Information of China (English)

    迟杰; 张雷; 吴万秀

    2011-01-01

    The potential function of hydrophytes for field bioremediation has been more and more concerned. In this work, a common hydrophyte, Hydrilla verticillata was selected to investigate its ability to remove α-HCH, γ-HCH and p,p'-DDT. The results showed that the biodegradation of α-HCH, γ-HCH and p,p'-DDT by Hydrilla verticillata followed first-order kinetics, and biodegradation rate constants were 0.086, 0.103 d-1 and 0.077 d-1, respectively. The bioconcentration factors of α-HCH, γ-HCH and p,p'-DDT by Hydrilla verticillata were significantly higher than 1, and in the order of p,p'-DDT>γ-HCH>α-HCH, α-HCH, γ-HCH and p,p'-DDT were mainly enriched in leaf. p,p'-DDD, the anaerobic product of p,p'-DDT, was detected in the experiment samples.%水生植物在水体污染修复中所起的作用越来越受到关注.采用室内培养方法,选取常见水生植物黑藻对α-HCH、γ-HCH和p,p′-DDT的去除特性进行了研究.结果表明,黑藻对α-HCH、γ-HCH和p,p′-DDT的降解符合一级反应动力学,降解速率常数分别为0.086、0.103 d-1和0.077 d-1;α-HCH、γ-HCH和p,p′-DDT在黑藻中的富集系数均显著大于1,顺序为p,p′-DDT>γ-HCH>α-HCH,主要富集在叶中;实验样品中均检出有p,p′-DDT的厌氧降解产物p,p′-DDD生成.

  8. Genetic Relationships among Invasive Hydrilla (Hydrilla verticillata L.f. Royle) Biotypes in the US and Their Implications for Management

    Science.gov (United States)

    2014-02-01

    1 US Army Engineer Research and Development Center, Vicksburg, MS. 2 Department of Biology , Texas Christian University, Fort Worth, TX. Report...2011). Experimental crosses have revealed that female dioecious plants in Florida are potentially fertile and can produce viable seed when pollinated by...Ecology Letters 13:145-153. Harms, N. E., Grodowitz, M. J. 2011. Overwintering biology of Hydrellia pakistanae, a biological control agent of

  9. Arsenic, Zinc, and Aluminium Removal from Gold Mine Wastewater Effluents and Accumulation by Submerged Aquatic Plants (Cabomba piauhyensis, Egeria densa, and Hydrilla verticillata

    Directory of Open Access Journals (Sweden)

    Ahmad Farid Abu Bakar

    2013-01-01

    Full Text Available The potential of three submerged aquatic plant species (Cabomba piauhyensis, Egeria densa, and Hydrilla verticillata to be used for As, Al, and Zn phytoremediation was tested. The plants were exposed for 14 days under hydroponic conditions to mine waste water effluents in order to assess the suitability of the aquatic plants to remediate elevated multi-metals concentrations in mine waste water. The results show that the E. densa and H. verticillata are able to accumulate high amount of arsenic (95.2% and zinc (93.7% and resulted in a decrease of arsenic and zinc in the ambient water. On the other hand, C. piauhyensis shows remarkable aluminium accumulation in plant biomass (83.8% compared to the other tested plants. The ability of these plants to accumulate the studied metals and survive throughout the experiment demonstrates the potential of these plants to remediate metal enriched water especially for mine drainage effluent. Among the three tested aquatic plants, H. verticillata was found to be the most applicable (84.5% and suitable plant species to phytoremediate elevated metals and metalloid in mine related waste water.

  10. Phytotoxicity and accumulation of zinc oxide nanoparticles on the aquatic plants Hydrilla verticillata and Phragmites Australis: leaf-type-dependent responses.

    Science.gov (United States)

    Song, Uhram; Lee, Sunryung

    2016-05-01

    The phytotoxicity and accumulation of zinc oxide nanoparticles (ZnO NPs) on aquatic plant Hydrilla verticillata and Phragmites australis were investigated using mesocosms. The percentage of dissolved Zn in the ZnO NP treatment solutions was measured along with plant shoot growth, antioxidant enzyme activity, chlorophyll content, and Zn content. The dissolution rate of ZnO NPs in Hoagland solution was inversely related to the concentration. The submerged aquatic plant H. verticillata, growth was reduced during the early stages of the experiment when exposed to the highest ZnO NP concentration (1000 mg/L), whereas the emerged aquatic plant P. australis began to show significantly reduced growth after a few weeks. The measurements of chlorophyll content, antioxidant enzyme activity, and Zn accumulation showed that P. australis was adversely affected by NPs and absorbed more Zn than H. verticillata. The results indicated that physiological differences among aquatic plants, such as whether they use leaves or roots for nutrient and water uptake, led to differences in nanoparticle toxicity. Overall, High ZnO NP concentrations caused significant phytotoxicity on aquatic plants, and low concentrations caused unpredictable phytotoxicity. Therefore, the use and disposal of zinc oxide nanoparticles should be carefully monitored.

  11. Arsenic, zinc, and aluminium removal from gold mine wastewater effluents and accumulation by submerged aquatic plants (Cabomba piauhyensis, Egeria densa, and Hydrilla verticillata).

    Science.gov (United States)

    Abu Bakar, Ahmad Farid; Yusoff, Ismail; Fatt, Ng Tham; Othman, Faridah; Ashraf, Muhammad Aqeel

    2013-01-01

    The potential of three submerged aquatic plant species (Cabomba piauhyensis, Egeria densa, and Hydrilla verticillata) to be used for As, Al, and Zn phytoremediation was tested. The plants were exposed for 14 days under hydroponic conditions to mine waste water effluents in order to assess the suitability of the aquatic plants to remediate elevated multi-metals concentrations in mine waste water. The results show that the E. densa and H. verticillata are able to accumulate high amount of arsenic (95.2%) and zinc (93.7%) and resulted in a decrease of arsenic and zinc in the ambient water. On the other hand, C. piauhyensis shows remarkable aluminium accumulation in plant biomass (83.8%) compared to the other tested plants. The ability of these plants to accumulate the studied metals and survive throughout the experiment demonstrates the potential of these plants to remediate metal enriched water especially for mine drainage effluent. Among the three tested aquatic plants, H. verticillata was found to be the most applicable (84.5%) and suitable plant species to phytoremediate elevated metals and metalloid in mine related waste water.

  12. Surveys for Biological Control Agents of Hydrilla verticillata in the People’s Republic of China in 2013

    Science.gov (United States)

    2017-03-01

    monoecious hydrilla is widely recognized as a relatively recent introduction. It was first discovered in Delaware in 1976 (Steward et al. 1984) and has...UNCLASSIFIED c. THIS PAGE UNCLASSIFIED 54 19b. TELEPHONE NUMBER (include area code) Standard Form 298 (Rev. 8-98) Prescribed by ANSI Std . 239.18

  13. Elaboración de abono orgánico a partir de plantas acuáticas: Elodea (Hydrilla verticillata) y Jacinto o Lirio de agua (Eichhornia crassipes), procedentes del Lago de Coatepeque y Lago de Güija

    OpenAIRE

    Reyes de Cabrales, Cecilia Elizabeth

    2009-01-01

    El presente estudio tiene por finalidad elaborar un abono orgánico bajo la técnica de compostaje, conocida como proceso de descomposición aeróbica. Se realizaron tres formulaciones de abono a partir de las plantas acuáticas Elodea (Hydrilla verticillata) y Jacinto de agua (Eichhornia crassipes) en las siguientes proporciones: Elodea 100%, Jacinto de agua 100% y mezcla de ambas en una proporción de 50:50. A los abonos obtenidos se les realizaron análisis químico con la finalidad de cuantificar...

  14. The Comparative Study on Photosynthesis of Hydrilla verticillata and Vallisneria natans in the Different Depth%黑藻与苦草在不同水深下光合作用的比较研究

    Institute of Scientific and Technical Information of China (English)

    经博翰; 袁龙义

    2014-01-01

    Chosen the Wagouzi site with abundant submerged population in the Honghu Lake as a sampling region, the difference of photosynthesis of submerged macrophytes Hydrilla verticillata( L. f. )Royle and vallisneria natans ( Lour. )Hara which are high resistance and obsorption to the dirty water in Honghu Lake was designed to research their response in the 100 cm and 50 cm water depth by Diving-PAM apparatus. The results showed that the value of External Transfer Rate(ETR)of Hydrilla verticillata and vallisneria natans separately approached to 25. 1 μmol· m-2 · s-1 and 10 . 1 μmol · m-2 · s-1 when the value of Photosynthetic Active Radiation( PAR )was stable for 342 μmol·m-2 · s-1 under the condition of 100 cm water depth. With the PAr intensity gradually becoming strong,ETr of Hydrilla verticillata reached its peak 21. 76 μmol·m-2 ·s-1 when the PAr value was 219 μmol· m-2 ·s-1 ,as well as ETr of vallisneria natans reached its peak 12. 65 μmol·m-2 ·s-1 when the PAr value was 515 μmol·m-2 ·s-1 . On the other hand,the value of ETr of Hydrilla verticillata and vallisneria natans separately approached to 26. 2 μmol·m-2 ·s-1 and 11. 9 μmol·m-2 ·s-1 when the value of PAr was stable for 515 μmol· m-2 ·s-1 under the condition of 50 cm water depth. With the PAr intensity gradually becoming strong,ETr of Hydrilla verticillata reached its peak 25. 27 μmol·m-2 ·s-1 when the PAr value was 219 μmol·m-2 ·s-1 ,as well as ETr of vallisneria natans reached its peak 4. 80 μmol·m-2 ·s-1 when the PAr value was 1 042 μmol· m-2 ·s-1 . In summary,no matter in 100 cm or 50 cm water depth condition,photosynthesis intensity of Hydrilla verticillata is stronger than that of vallisneria natans.%以沉水植物较为丰富的洪湖凹沟子作为采样区域,利用水下调制荧光仪研究洪湖常见的2种高耐污、高吸污水鳖科沉水植物黑藻( Hydrilla verticillata( L. f.)Royle)与苦草( vallisneria natans( Lour

  15. EFFECT OF LIGHT INTENSITY, TEMPERATURE, TOTAL NITROGEN CONCENTRATION AND THEIR INTERACTION ON HYDRILLA VERTICILLATA%光强、温度、总氮浓度对黑藻生长的影响

    Institute of Scientific and Technical Information of China (English)

    朱丹婷; 乔宁宁; 李铭红; 陈攀

    2011-01-01

    为寻求沉水植物生长的主效环境因子,探求富营养化水体沉水植物的衰亡机理并选择治理富营养化的先锋植物,实验选择长江中下游常见沉水植物种黑藻(Hydrilla verticillata),利用正交试验设计,通过室内静态模拟实验研究三种主要环境因子光照强度、温度、总氮浓度及其互作对黑藻断枝生长的影响.结果表明:光照强度和温度为影响黑藻生长的主效环境因子,并且光强与温度的交互作用对黑藻生长有较为显著的影响,具体表现为黑藻的生长指标(株高、生物量、分枝数)、光合指标(叶绿素a+b浓度、叶绿素a/b、叶绿体总色素含量)和生理活性指标(根活力、可溶性糖含量、MDA含量)的变化均与这两个环境因子及其互作呈显著相关;总氮浓度的变化对黑藻生长影响不大,在2-8 mg/L的总氮浓度下,黑藻均可以正常生长.根据本实验黑藻生长指标、光合色素含量以及生理活性在不同环境因子组合的变化结果可知,黑藻在5320-12000 lx光照强度、20℃-30℃、4-8 mg/L水体总氮浓度的条件下生长良好,故推测黑藻可作为春夏季富营养化水体中恢复和重建沉水植被的先锋工具种.%With the strengthening of freshwater eutrophication, the original aquatic vegetation gradually reduced and even disappeared, resulting in the well-functional grass type water degraded to algae-based water. Submerged plant is a key factor for the construction of stable and well-functioning freshwater ecosystems. Therefore, restoration and reconstruction of submerged plants is very important for prevention and control of eutrophication. The growth and development, decline and extinction of submerged plants are closely related with environmental factors due to their aquatic characteristics. In this study, we aim to explore the effects of light intensity, temperature, total nitrogen concentration and their interactions on the growth and development

  16. 两种沉水植物黑藻和伊乐藻的种间竞争%INTER-SPECIFIC COMPETITION BETWEEN TWO SUBMERGED MACROPHYTES, ELODEA NUTTALLII AND HYDRILLA VERTICILLATA

    Institute of Scientific and Technical Information of China (English)

    许经伟; 李伟; 刘贵华; 张利静; 刘文治

    2007-01-01

    采用取代系列实验方法,主要从竞争期的长短出发,研究了黑藻(Hydrilla verticillata)和伊乐藻(Elodea nuttallii)的种间竞争关系,并考查了在不同底质(土壤)肥力下两者种间竞争能力的变化情况.实验发现,伊乐藻由于具有较强的耐寒能力,在冬春时空竞争方面占有明显的优势,从而在周年实验中表现出较强的竞争优势,取代黑藻生长.而在短期实验中,黑藻由于可在水面生长形成较上位的冠层的特性,与伊乐藻相比在水体上层空间占领和阳光获取方面具有一定的优势,因此造成两种间竞争的不平衡,竞争偏利于黑藻,且这种优势随底质(土壤)肥力的增加而有所增强,但并没有明显取代现象的发生,两物种可以在混合种群中共存.

  17. Combining Plant Pathogenic Fungi and the Leaf-Mining Fly, Hydrellia pakistanae, Increases Damage to Hydrilla

    OpenAIRE

    2003-01-01

    Four fungal species, F71PJ Acremonium sp., F531 Cylindrocarpon sp., F542, Botrytis sp., and F964 Fusarium culmorum [Wm. G. Sm.] Sacc. were recovered from hydrilla [ Hydrilla verticillata (L. f.) Royle] shoots or from soil and water surrounding hydrilla growing in ponds and lakes in Florida and shown to be capable of killing hydrilla in a bioassay. The isolates were tested singly and in combination with the leaf-mining fly, Hydrellia pakistanae ...

  18. 沉水植物轮叶黑藻附生细菌对双酚A的降解能力研究%Degradation of bisphenol A by the epiphytic bacteria of submerged macrophytes Hydrilla verticillata (L. f.) Royle

    Institute of Scientific and Technical Information of China (English)

    张国森; 王玉; 庄晓瑾; 杨劭; 蒋金辉

    2016-01-01

    Epiphytic bacteria of submerged macrophytes may have the capability in biodegradation and/or biotransformation of bisphenol A (BPA) in water column, therefore affect the fate of such environmental pollutant. In this research,Hydrilla verticillata(L. f.) Royle was selected and their attached BPA degrading epiphytic bacteria attached were isolated. Among the 22bacteria strains, the BPA removal rates were from 11.46% to 25.06% with the inoculum density at 1×10-8cell/mL and culture at 37℃ for 72h. The most effective bacteria strains, B12, B14and B23 were identified asLysinibacillus sp.,Brevibacterium sp. andOchrobactrum sp., respectively, according to the results of 16S rDNA sequencing and morphological, physiological and biochemical tests. But aseptic seedlings ofH. verticillata significantly decreased their BPA removal rates after the addition with B12, B14and B23 (P<0.05). Natural seedlings of such species surprisingly increased about 5% in BPA removal after partially removing their epiphyte with physical methods. All the results indicated that epiphytic bacteria of submerged plant can remove BPA, although their contributions (about 23%) are less than the host plants in the submerged macrophytes-epiphyte associations.%沉水植物附生细菌可能具有降解转化水体中双酚 A(BPA)能力从而影响该污染物在环境中的归趋.以轮叶黑藻为代表,分离筛选其BPA降解附生菌,结果共获得22株,在接种量为1×108个/mL,37℃下72h对BPA的去除率为11.46%~25.06%.选择降解率最高的3株细菌B12、B14和B23,采用16S rDNA鉴定,结合生理生化反应和形态观察,3株细菌分别为属于Lysinibacillus sp.(杆菌属),Brevibacterium sp.(短杆菌属)和Ochrobactrum sp.(苍白杆菌属).将3株菌株添加至轮叶黑藻无菌苗体系中,发现BPA去除率显著下降(P<0.05).物理去除部分野生轮叶黑藻表面部分附生细菌后,BPA去除率反而上升(约5%).综合本研究结果,沉水植物附生细菌具有降

  19. Eficácia do diquat no controle de Hydrilla verticillata, Egeria densa e Egeria najas e toxicidade aguda para o Guaru (Phallocerus caudimaculatus, em condições de laboratório Efficacy of diquat in the control of Hydrylla verticillata, Egeria densa and Egeria najas and its acute toxicity to Phallocerus caudimaculatus, under laboratory conditions

    Directory of Open Access Journals (Sweden)

    M.N.P. Henares

    2011-06-01

    Full Text Available No Brasil, as macrófitas aquáticas submersas, Egeria densa e Egeria najas, têm causado prejuízos aos usos múltiplos da água. Hydrilla verticillata foi recentemente introduzida, mas tem histórico como planta problemática nos EUA, no México e na Austrália. O objetivo deste trabalho foi avaliar as suscetibilidades relativas dessas três macrófitas aquáticas ao diquat e os riscos da utilização desse herbicida para o guaru (Phallocerus caudimaculatus. Para isso, foram instalados ensaios em condições de laboratório, a fim de avaliar a suscetibilidade relativa das três macrófitas por meio da manutenção de ponteiros dessas plantas em soluções contendo 0,0; 0,2; 0,4; 0,8; e 1,6 mg L-1 de diquat (Reward® por 14 dias. A avaliação foi realizada pela variação do acúmulo de matéria fresca e do comprimento dos ponteiros no período de exposição ao herbicida. H. verticillata mostrou maior sensibilidade ao diquat em comparação com as duas macrófitas do gênero Egeria, mesmo em baixas concentrações do herbicida. Nas maiores concentrações, E. densa mostrou maior sensibilidade que E. najas. O risco da aplicação do diquat para P. caudimaculatus foi estimado pela toxicidade aguda. Alevinos de P. caudimaculatus de 0,4 ± 0,2 mg foram expostos a soluções de 0,0; 1,0; 5,0; 10,0; 15,0; 20,0; 25,0; e 30,0 mg L-1 de diquat. A concentração letal de 50% (CL(I (50;96h do diquat estimada para P. caudimaculatus foi de 7,17 mg L-1. Para P. caudimaculatus, a toxicidade aguda foi superior à concentração recomendada para o controle de macrófitas aquáticas submersas, indicando risco muito baixo para esse peixe.In Brazil, the submerged plants Egeria densa and Egeria najas have caused damage to multiple uses of water. Hydrilla verticillata has been recently introduced, but it has a history as a problem plant in the U.S., Mexico and Australia. The objectives of this work were to assess the relative susceptibilities of these three

  20. Effects of arsenic speciation on the phytochelatins (PCs) synthesis by Hydrilla verticillata%不同形态砷对黑藻植物络合素合成的影响

    Institute of Scientific and Technical Information of China (English)

    李红梅; 王宏镔; 王海娟; 宋雁辉; 钟正燕

    2012-01-01

    采用反相高效液相色谱(RP-HPLC)法,研究了水培条件下3种形态砷[三价砷As(Ⅲ)、五价砷As(Ⅴ)和二甲基砷(DMA)]的5个处理浓度(0、0.3、1.0、3.0和5.0 mg·L-1)对黑藻(Hydrilla venicillata(L.f.)Royle)砷吸收和植物络合素(PCs)合成的影响.结果表明:黑藻对As表现出明显的吸收和富集效应,低浓度As能促进黑藻生长,对As (Ⅲ)的吸收显著高于As(Ⅴ)和DMA;As(Ⅲ)和As(Ⅴ)处理显著诱导了谷胱甘肽(GSH)、PC2和PC4的合成,且与黑藻体内As含量呈显著正相关(P<0.05);PCs对DMA处理不敏感,只有在0.3 mg·L-1下能合成PCs;在As( Ⅲ)处理下,GSH和PC2的合成随As(Ⅲ)浓度的增加呈先增加后减少的趋势,而PC4的合成却随As(Ⅲ)浓度的增加而增加;在As(Ⅴ)处理下,GSH的合成随As(Ⅴ)浓度的增加呈先增加后减少的趋势,而PG2和PC4的合成则随As(Ⅴ)浓度的增加而增加;在3种形态As的不同浓度处理中PC3的合成很少,只在0.3 mg·L-1下有少量合成.研究结果表明,PCs对As( Ⅲ)和As(Ⅴ)的胁迫较敏感,可选择性地作为这2种形态砷胁迫下黑藻的生物标记物.%A hydroponie experiment was conducted to study the effects of different arsenic species [As(III), As(V), and DMA] at five concentrations (0, 0.3, 1.0, 3.0, and 5.0 mg· L-1) on the arsenic uptake and phytochelatins (PCs) synthesis by Hydrilla verticillaia. The PCs contents were identified by reversed phase-high performance liquid chromatography ( RP-HPLC). H. vertkillata had an obvious arsenic uptake and accumulation. Low concentrations arsenic promoted the growth of H. veniciilata, and the uptake of As( III) by H. vertkillata was significantly higher than that of As(V) or DMA. As(III) and As(V) promoted the synthesis of GSH, PC2, and PC4 significantly, and the synthesis was significantly positively correlated with the arsenic concentration in H. venkillala (P<0.05). DMA had little effects on the synthesis of PCs, with a detectable synthesis

  1. Exploration for Natural Enemies of Hydrilla verticillata in Eastern Africa.

    Science.gov (United States)

    1980-05-01

    to Lake Baringo fol- lowed by Lake Naivasha bef6rb returning to Nairobi. Habitats Examined 25. The types of habitats surveyed were (a) rivers, (b... Lake Baringo , Baringo Silted and mud Typha sp. Province, Kenya brown from erosion Lake Naivasha, Photozone 1.5 m, mud Cyperus papyrus, Nakuru...consisted of two phasesi (a)a-vide survey through Tanzania and Kenya, followed by +*r’an intense survey of Lake Tanganyika, including a preliminary study

  2. Competition Between Hydrilla verticillata and Vallisneria americana Under Different Environmental Conditions

    Science.gov (United States)

    1994-03-01

    interesting that this occurred only under the low fertility condition, since it has been suggested that foliar uptake and translocation of solution K to...ill. ; 28 cm. - (Technical report ; A-94-1 ) Includes bibliographic references. 1. Plant biomass. 2. Plant competition. 3. Fertilization of plants. I...of canopy-forming submersed aquatic plants, provide a habitat that is generally detrimental to fish and other desirable aquatic organisms (Newroth

  3. Surveys for Pathogens of Monoecious Hydrilla

    Science.gov (United States)

    2014-01-01

    purpose of the study presented herein was to survey some known populations of monoecious hydrilla and isolate potential fungal pathogens. MATERIALS ...sporulating species herein noted as dematiaceous (dark mycelium ) or moniliaceous (hyaline mycelium ) Ascomycetes (Table 1). The majority of the species

  4. Preliminary Testing of Mycoleptodiscus terrestris Formulations

    Science.gov (United States)

    2009-03-01

    submersed macrophyte , Hydrilla verticillata (L.f.) Royle (hydrilla). BACKGROUND: As herbicide resistance becomes an increasing problem worldwide...Mycoleptodiscus terrestris, as a biocontrol agent for the management of Myriophyllum spicatum in Lake Guntersville Reservoir . Technical Report A-96-4

  5. Using Remote Sensing and Spatial Information Technologies to Detect and Map Two Aquatic Macrophytes

    OpenAIRE

    Everitt, J.H.; Yang, C.; Escobar, D.E.; Webster, C.F.; Lonard, R.I.; Davis, M.R.

    1999-01-01

    This paper describes the light reflectance characteristics ofwaterhyacinth [Eichhornia crassipes (Mort.) Solms] and hydrilla [Hydrilla verticillata (L.F.) Royle] and the application of airborned videography with global positioning system (GPS) and geographic information system (GIS) technologies for distinguishing and mapping the distribution of these two aquatic weeds in waterways of southern Texas. Field reflectance measurements made at several locations showed that waterhyacinth generall...

  6. Surveys for Pathogens of Monoecious Hydrilla 2014

    Science.gov (United States)

    2016-06-01

    hypothesized that the growth and reproductive habits were adaptations to northern climates, which suggests a temperate origin of the plant that was...it was only mildly pathogenic; but when it was inoculated onto plants that were endophyte-infected, the stressed plants usually died. These...Netherland, and Zack Banks for collecting monoecious hydrilla samples. Thanks also go to Michael Grodowitz and Lynde Dodd for reviewing the manuscript

  7. Aquatic Plant Control Research Program. Effects of Organic Amendments to Sediment on Freshwater Macrophyte Growth.

    Science.gov (United States)

    1983-10-01

    The growth of three submersed macrophyte species (Myriophyllum spicatum, Hyjdri11a verticillata, and Elodea canadensis) and three partially emergent... Elodea canadensis Rich. in Michx; and Hydrilla verticillata (L.f.) Caspary. Nomenclature follows Godfrey and Wooten (1979, 1981). The first two species

  8. Surveys for Pathogens of Monoecious Hydrilla in 2013

    Science.gov (United States)

    2014-10-01

    2013, monoecious hydrilla was collected in the field from Strom Thurmond Reservoir in South Carolina/ Georgia , Lake Guntersville in Alabama, and Lake...Gainesville, Florida where monoecious hydrilla plants collected in Missouri, Kansas, South Carolina/ Georgia (Strom Thurmond Reservoir), and North...species” was plated onto Potato Carrot Agar (PCA; Dhingra and Sinclair 1995) and PDA and incubated at 25 oC under a grow light (plant aquarium wide

  9. Mechanizing the Metatheory of LF

    CERN Document Server

    Urban, Christian; Berghofer, Stefan

    2008-01-01

    LF is a dependent type theory in which many other formal systems can be conveniently embedded. However, correct use of LF relies on nontrivial metatheoretic developments such as proofs of correctness of decision procedures for LF's judgments. Although detailed informal proofs of these properties have been published, they have not been formally verified in a theorem prover. We have formalized these properties within Isabelle/HOL using the Nominal Datatype Package, closely following a recent article by Harper and Pfenning. In the process, we identified and resolved a gap in one of the proofs and a small number of minor lacunae in others. Besides its intrinsic interest, our formalization provides a foundation for studying the adequacy of LF encodings, the correctness of Twelf-style metatheoretic reasoning, and the metatheory of extensions to LF.

  10. Spectral signatures of hydrilla from a tank and field setting

    Institute of Scientific and Technical Information of China (English)

    Alfonso BLANCO; John J.QU; William E.ROPER

    2012-01-01

    The invasion of hydrilla in many waterways has caused significant problems resulting in high maintenance costs for eradicating this invasive aquatic weed.Present identification methods employed for detecting hydrilla invasions such as aerial photography and videos are difficult,costly,and time consuming.Remote sensing has been used for assessing wetlands and other aquatic vegetation,but very little information is available for detecting hydrilla invasions in coastal estuaries and other water bodies.The objective of this study is to construct a library of spectral signatures for identifying and classifying hydrilla invasions.Spectral signatures of hydrilla were collected from an experimental tank and field locations in a coastal estuary in the upper Chesapeake Bay.These measurements collected from the experimental tank,resulted in spectral signatures with an average peak surface reflectance in the near-infrared (NIR) region of 16% at a wavelength of 818 nm.However,the spectral measurements,collected in the estuary,resulted in a very different spectral signature with two surface reflectance peaks of 6% at wavelengths of 725 nm and 818 nm.The difference in spectral signatures between sites are a result of the components in the water column in the estuary because of increased turbidity (e.g.,nutrients,dissolved matter and suspended matter),and canopy being lower (submerged) in the water column.Spectral signatures of hydrilla observed in the tank and the field had similar characteristics with low reflectance in visible region of the spectrum from 400 to 700 nm,but high in the NIR region from 700 to 900 nm.

  11. Overseas Surveys (1981-1983) for Insects to Control Hydrilla.

    Science.gov (United States)

    1985-12-01

    several treatments usually required during the growing season, only high priority waters can be effectively managed. Taxonomy Identification difficulties...11. Hydrilla has been recognized as a separate species of plant since the early days of taxonomy . According to Cook and Luond’s (1982) synonomy...Order Tanaidacea Tanaids Philippines 5 LUZ32Z1, LUZ832Z2 Total 105 Glass Mollusca Order Gastropoda (Snails) Ampullariidae Burma 3 BUR82204 Malaysia I

  12. Optimal treatment increased the seed germination of Salvia verticillata L.

    Directory of Open Access Journals (Sweden)

    ALALEH KHAKPOOR

    2015-12-01

    Full Text Available Most seeds of the medicinal species are variable regarding their ecological compatibility with environmental conditions. Therefore, identifying the ecophysiological factors that affect dormancy and create optimal conditions for seed germination of medicinal plants is necessary for their culture and production. To evaluate the effect of different treatments on seed germination of medicinal species of Salvia verticillata, collected in the summer of 2010 in Eastern Azarbaijan, we have performed completely randomized experimental tests with 4 replications. The experimental design of treatment prior to growth included: scrape the skin with sandpaper, treatment with 500 ppm gibberellic acid for 24 and 48 h, treatment with citric acid for 10, 20 and 30 minutes, chilling for 2 and 4 weeks, treatment with warm water at 70°C and control treatment. Results showed that the effect of different treatments was significant on seed germination percent of the medicinal plant Salvia verticillata. Scrape the skin with sandpaper, citric acid treatment for 10, 20 and 30 minutes, and gibberellic acid treatment for 24 hours, increased the germination percentage compared to the control treatment. The most positive impact was observed on the dormancy breaking and germination of medicinal species Salvia verticillata.

  13. Nystatin LF (Aronex/Abbott).

    Science.gov (United States)

    Arikan, S; Rex, J H

    2001-04-01

    Nystatin LF (Nyotran) is a liposomal, intravenous nystatin formulation under development by Aronex, under license from the MD Anderson Cancer Research Center, as a systemic antifungal agent against strains including Aspergillus and Candida. Like amphotericin, nystatin is a polyene derivative that binds to ergosterol, a fungal cell membrane component, creating a pore in the membrane and thus killing the cell. Nystatin is an established antifungal agent, but is restricted to topical use as it is ineffective orally and severely toxic when administered iv [187583], [187589]. It has demonstrated good, broad in vitro antifungal activity against clinically relevant filamentous fungi [319465], including fungi resistant to fluconazole and amphotericin B products [264505], [2869821, [287790], 1289522]. The company is also conducting a phase III trial to evaluate its efficacy against cryptococcal meningitis [305531], [334156]. Aronex filed for approval of nystatin LF in Spain in December 1997 [272986] and expected to file an NDA in the US by the end of 1999 1311208], [342003]. However, in an effort to ensure that its US and European filings contained data from the phase III cryptococcal meningitis trial in its entirety, Aronex's marketing partner requested that all the 70-day data be gathered prior to unblinding this study. The filing had initially been based on interim data at the 14- and 21-day endpoint 1344887]. In September 2000, the company anticipated an NDA filing in the US in the fourth quarter of 2001 1382861], 1387947]. In December 1997, Aronex, together with Grupo Ferrer Internacional, filed an MAA in Spain seeking approval for Nyotran for the treatment of systemic fungal infections. Aronex intended to follow the filing with additional filings in other European countries 1272986]. In 1997, a commercialization agreement was signed with Ferrer for Spain and Portugal, with Aronex intending to form other such partnerships throughout Europe and Asia 1248346]. In

  14. A Practical Module System for LF

    DEFF Research Database (Denmark)

    Schürmann, Carsten; Rabe, Florian

    2009-01-01

    morphisms, which provide a semantically transparent module level and permit to represent logic translations as homomorphisms. Modular LF is a conser- vative extension over LF, and integrates an elaboration of modular into core LF signatures. We have implemented our design in the Twelf system and used......Module Systems for proof assistants provide administrative support for large developments when mechanizing the meta-theory of programming languages and logics. In this paper we describe a module system for the logical framework LF. It is based on two main primitives: signatures and signature...... it to modularize large parts of the Twelf example library....

  15. Phytofabrication of silver nanoparticles by using aquatic plant Hydrilla verticilata

    Directory of Open Access Journals (Sweden)

    MAHENDRA RAI

    2012-07-01

    Full Text Available Sable N, Gaikwad S, Bonde S, Gade A, Rai M. 2012. Phytofabrication of silver nanoparticles by using aquatic plant Hydrilla verticilata. Nusantara Bioscience 4: 45-49. In the context of current drive to developed new green technology in nanomaterials, synthesis of nanoparticles is of considerable importance. There has been considerable work done in the field of nanoscience and nanotechnology during the last decade due to the introduction of various protocols for the synthesis of nanoparticles by using plants and microorganisms. Here we firstly report the extracellular phytosynthesis of silver nanoparticles (Ag-NPs using aquatic plants Hydrilla verticilata. The characterization of the phytosynthesized Ag-NPs was done with the help of UV-Vis spectroscopy, FTIR, Nanoparticle Tracking Analysis (NTA, Zeta potential and SEM. The SEM micrograph revealed the synthesis of polydispersed spherical nanoparticles, with the average size of 65.55 nm. The phytofabricated Ag-NPs can be used in the field of medicine and agriculture, due to their antimicrobial potential.

  16. Cytotoxic activity of the essential oil of Salvia verticillata L.

    Directory of Open Access Journals (Sweden)

    N. Khosravi Dehaghi

    2014-09-01

    Full Text Available Salvia is one of the largest genera of  Lamiaceae family. Several species of this genus are perfumed and wealthy in essential oils. Some of them are used in industry, pharmacy and aromatherapy. They have shown different biological effects such as antibacterial and antioxidant activity. For the present study, Salvia verticillata L. was collected from Shahrestanak, Mazandaran, Iran. Hydrodistilled essential oil from the aerial parts of this plant was obtained with a Clevenger type  apparatus  and was analyzed by GC and GC/MS. Moreover, the cytotoxic activity of the essential oil was investigated against HT-29 (colon adenocarcinoma, Caco-2 (colorectal adenocarcinoma, T-47D (breast ductal carcinoma and NIH-3T3 (Swiss mouse embryo fibroblast cell lines by MTT test. 59 components were characterized from the oil with trans-caryophyllene (24.40%, β-phellandrene (9.08%, α-humulene (8.61%, bicyclogermacrene (6.32%, spathulenol (5.89% and β-pinene (5.00% as the major constituents. These compounds represented 97.67% of the essential oil and included monoterpenes (34.83% and sesquiterpens (61.84%. The results of the cytotoxicity assay demonstrated that the essential oil of S. verticillata showed higher cytotoxic effect on Caco-2 cell line.

  17. Final Critical Habitat for Schiedea verticillata from the Northwestern Hawaiian Islands, Hawaii

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — To provide the user with a general idea of areas where final critical habitat for Schiedea verticillata known historically from the Northwestern Hawaiian Islands...

  18. DNA analysis of the genes encoding acidocin LF221 A and acidocin LF221 B, two bacteriocins produced by Lactobacillus gasseri LF221

    NARCIS (Netherlands)

    Majhenič, A.Č.; Venema, K.; Allison, G.E.; Matijašić, B.B.; Rogelj, I.; Klaenhammer, T.R.

    2004-01-01

    Lactobacillus gasseri LF221, an isolate from the feces of a child, produces two bacteriocins. Standard procedures for molecular techniques were used to locate, clone and sequence the fragments of LF221 chromosomal DNA carrying the acidocin LF221 A and B structural genes, respectively. Sequencing ana

  19. DNA analysis of the genes encoding acidocin LF221 A and acidocin LF221 B, two bacteriocins produced by Lactobacillus gasseri LF221

    NARCIS (Netherlands)

    Majhenič, A.Č.; Venema, K.; Allison, G.E.; Matijašić, B.B.; Rogelj, I.; Klaenhammer, T.R.

    2004-01-01

    Lactobacillus gasseri LF221, an isolate from the feces of a child, produces two bacteriocins. Standard procedures for molecular techniques were used to locate, clone and sequence the fragments of LF221 chromosomal DNA carrying the acidocin LF221 A and B structural genes, respectively. Sequencing ana

  20. Antibacterial activity of lichen Usnea rubrotincta, Ramalina dumeticola, and Cladonia verticillata

    Science.gov (United States)

    Gunasekaran, Saranyapiriya; Rajan, Vinoshene Pillai; Samsudin, Mohd. Wahid; Din, Laily; Ramanathan, Surash; Murugaiyah, Vikneswaran

    2015-09-01

    The present study was carried out to evaluate the antibacterial activity of extract and chemical constituents of Usnea rubrotincta, Ramalina dumeticola and Cladonia verticillata. Extracts of U. rubrotincta and R. dumeticola showed promising antibacterial activity against gram positive bacteria Staphylococcus aureus and Bacillus subtilis. The lowest value of MIC (15.63 μg/mL) was observed for the acetone extract of U. rubrotincta against B. subtilis. While extract of C. verticillata was neither active against gram positive nor gram negative bacteria at the highest tested concentration of 500 μg/m. This is the first evaluation of antibacterial activity of lichens found in Malaysia and to our knowledge, this is the first report of antibacterial

  1. Verticillosides A-M: Polyoxygenated pregnane glycosides from Asclepias verticillata L.

    Science.gov (United States)

    Araya, Juan J; Binns, Franklin; Kindscher, Kelly; Timmermann, Barbara N

    2012-06-01

    As part of our ongoing effort to explore the chemical diversity of plants of the United States Midwest region, the isolation and identification of 13 pregnane glycosides named verticillosides A-M from Asclepias verticillata L. are reported. The structures of these compounds were elucidated by various spectroscopic techniques, including 1D and 2D NMR, IR, UV, and HRMS. The cytotoxicity of the isolates was evaluated against paired breast cell lines Hs578T (cancer) and Hs578Bst (normal), however, no significant growth inhibition was observed.

  2. Correlation of Biomicroscopic Findings with Confocal Microscopy in Eyes with Amiodarone-Induced Cornea Verticillata

    Directory of Open Access Journals (Sweden)

    Emine Kaya

    2014-01-01

    Full Text Available Objectives: To investigate the correlation between biomicroscopic and confocal microscopic findings in eyes with amiodarone-induced cornea verticillata. Materials and Methods: Sixteen eyes of 8 patients with amiodarone-induced cornea verticillata were evaluated. Eyes with keratopathy were staged according to Orlando slit-lamp microscopy classification. Confocal laser-scanning microscopy was performed by Rostock cornea modulated to HRT II (Heidelberg Engineering GmbH, Heidelberg, Germany, and staging was done according to Falke’s classification that is based on the degree of epithelial basal cell deposit accumulation. The relation between biomicroscopic staging and corneal involvement detected on confocal microscopy was assessed by Spearman correlation analysis. Results: The mean age of the 8 patients (5 male, 3 female was 63.1±7.2 (50 to 69 years. The mean duration of drug treatment was 12.1±11.8 (3 to 36 months, and the mean drug treatment dose was 312.5±223.2 (100 to 800 mg/day. At the time of examination, 50% of the patients had already given up the treatment at a mean of 29.5±15.8 (6 to 40 months ago, whereas the other 50% were still on amiodarone therapy. Hyper-reflecting deposits were observed in the basal epithelium, anterior-, mid-and deep-stroma, and in the endothelium on confocal microscopic examination. Correlation was detected between biomicroscopic and confocal microscopic stages (r=0.770, p<0.001. Frequency of detecting deposits in the stroma and endothelium was found to be increasing as the biomicroscopic stage increased (r=0.844; p<0.001 and r=0.551; p<0.01, respectively. Conclusion: In amiodarone-induced cornea verticillata, correlated results were detected between biomicroscopic and confocal microscopic staging. Therefore, in clinics where confocal microscopy is not available, biomicroscopic staging can be used as a guiding parameter in eyes with amiodarone-induced cornea verticillata. (Turk J Ophthalmol 2014; 44: 63-67

  3. Low-Fiend Vector Magnetometer (V-400-LF) Project

    Data.gov (United States)

    National Aeronautics and Space Administration — This 2010 NASA SBIR Phase 1 proposal for an innovative Low-Field Vector Magnetometer (V-400-LF) is a response to subtopic S1.06 Particles and Field Sensors and...

  4. A trnI_CAU triplication event in the complete chloroplast genome of Paris verticillata M.Bieb. (Melanthiaceae, Liliales).

    Science.gov (United States)

    Do, Hoang Dang Khoa; Kim, Jung Sung; Kim, Joo-Hwan

    2014-06-19

    The chloroplast is an essential plant organelle responsible for photosynthesis. Gene duplication, relocation, and loss in the chloroplast genome (cpDNA) are useful for exploring the evolution and phylogeny of plant species. In this study, the complete chloroplast genome of Paris verticillata was sequenced using the 454 sequencing system and Sanger sequencing method to trace the evolutionary pattern in the tribe Parideae of the family Melanthiaceae (Liliales). The circular double-stranded cpDNA of P. verticillata (157,379 bp) consists of two inverted repeat regions each of 28,373 bp, a large single copy of 82,726 bp, and a small single copy of 17,907 bp. Gene content and order are generally similar to the previously reported cpDNA sequences within the order Liliales. However, we found that trnI_CAU was triplicated in P. verticillata. In addition, cemA is suspected to be a pseudogene due to the presence of internal stop codons created by poly(A) insertion and single small CA repeats. Such changes were not found in previously examined cpDNAs of the Melanthiaceae or other families of the Liliales, suggesting that such features are unique to the tribe Parideae of Melanthiaceae. The characteristics of P. verticillata cpDNA will provide useful information for uncovering the evolution within Paris and for further research of plastid genome evolution and phylogenetic studies in Liliales.

  5. The European VLF/LF radio network: current status

    Science.gov (United States)

    Biagi, Pier Francesco; Maggipinto, Tommaso; Ermini, A.

    2014-11-01

    For several years researches about correlation between seismicity and disturbances in radio broadcasting are being carried out: in particular, the Japanese Pacific VLF radio network and the European VLF-LF radio network have been developed during the last years. The European network has been developed starting from two LF receivers located in central Italy in 1996. Up to now, 11 receivers of a new type, able to sample the VLF and LF intensity of ten radio signals, are being into operation in different European countries. The daily updating of data is effective and the data bank is located at the Department of Physics of the University of Bari (Italy) which is the central node of the network. In order to discover anomalies, the software able to carry out automatically a daily data analysis by the Wavelet spectra method has been planned and realized. At the moment, the software operates on four signals (two LF and two VLF) collected by one of the receiver located in Italy. If the anomaly is particularly strong a warning system gives an advise on the work station into operation in the central node of the Network. In any case, before assuming an anomaly as a seismic anomaly, geomagnetic and meteorological data must be checked as well as any possible instrumental malfunction. At present these controls are carried out only discontinuously by the researchers of the Bari Team.

  6. Novel GLA Deletion in a Cypriot Female Presenting with Cornea Verticillata

    Directory of Open Access Journals (Sweden)

    Theodoros Georgiou

    2016-01-01

    Full Text Available Fabry disease is an X-linked lysosomal storage disorder resulting from a deficiency of the hydrolytic enzyme α-galactosidase A (α-Gal-A. It is characterized by progressive lysosomal accumulation of globotriaosylceramide (Gb3 and multisystem pathology, affecting the skin, nervous and cerebrovascular systems, kidneys, and heart. Heterozygous females typically exhibit milder symptoms and a later age of onset than males. Rarely, they may be relatively asymptomatic throughout a normal life span or may have symptoms as severe as those observed in males with the classic phenotype. We report on a 17-year-old female in whom cornea verticillata was found during a routine ophthalmological examination but with no other clinical symptoms. Leucocyte α-galactosidase activity was within the overlap range between Fabry heterozygotes and normal controls. Sanger sequencing of the GLA gene failed to reveal any pathogenic variants. Multiplex Ligation-dependent Probe Amplification (MLPA analysis revealed a deletion of exon 7. Using a long-range PCR walking approach, we managed to identify the deletion breakpoints. The deletion spans 1182 bp, with its 5′ end located within exon 6 of the GLA gene and its 3′ end located 612 bp downstream of exon 7. This finding represents a novel deletion identified in the first reported Cypriot female carrier of Fabry disease.

  7. Experimental Study on Deep Desulfurizer in LF Process

    Institute of Scientific and Technical Information of China (English)

    WU Long; PEI Fen; CHEN Yut; LI Shi-qi

    2012-01-01

    CaO-Al2O3-SiO2-CaF2-MgO was selected as the slag system for desulfurization in LF process.The reaction between steel and slag during desulfurization has been simulated by using Factsage software to study the influence of component on the sulfur distribution ratio.In order to research the influence of CaO content,aluminum powder content and its granularity on desulfurization,laboratory experiments have been carried out in a 200 kg inductive furnace.Results showed that the optimal composition of deep desulfurizer is wCaO=64% and aluminium powder 10% with a granularity of 30 μm.Industrial trials showed that the main composition range of final slag in LF process is wCaO=53.0%-57.0%,wAl2O3=23.4%-25.1%,wSiO2=8.1%-10.0%,and wCaF2=3.2%-4.7%.The sulfur mass percent in steel is lower than 0.0008% with a desulfurization rate above 89%.According to the result of industrial production,this desulfurizer could meet the production requirement for ultra-low sulfur steel,of which sulfur mass percent is under 0.0015%

  8. Anatomy and histochemistry of the vegetative organs of Cissus verticillata: a native medicinal plant of the Brazilian Amazon

    Directory of Open Access Journals (Sweden)

    Andréia B. de Oliveira

    2012-12-01

    Full Text Available The purpose of this paper was to carry out an anatomical and histochemical analysis of the vegetative organs of Cissus verticillata (L. Nicolson & C.E. Jarvis, Vitaceae, to contribute for the attest the taxonomic identity of the medicinal plant. Samples from root, stem, leaf and tendril were cleared, dissociated and processed according to the usual methodology for observation under light and scanning electron microscopes. Histochemical tests were performed in order to identify polysaccharides, phenolic and lipid compounds. The C. verticillata root is typically protostelic, and the stem is eustelic with collateral bundles. The tendril presents structural organization similar to the stem, suggesting a common origin for both. The petiole has an epidermis with ornamented cuticle; the cortex is composed of collenchyma and parenchyma, and the vascular tissues are arranged in collateral bundles. The leaf blade is amphistomatic with non-glandular and glandular trichomes, and the mesophyll is dorsiventral. The identification of the idioblasts as secretion site of the phenolic compounds, mucilage and terpenoids as being responsible for the potential activity of the plant is of fundamental importance for future bioprospecting research on this species.

  9. Cloning and CharaCterization of theStrictosidine-β-D-glucosidase(SGD)Gene from Rauvolfia verticillata

    Institute of Scientific and Technical Information of China (English)

    Hongxuan XU; Kai CHANG; Lili MA; Yue ZHENG; Xiaoqiang LIU

    2012-01-01

    [Objective] This study aimed to clone Scrictosidine-~-D-glucosidase (SGD) gene from Rauvolfia verticillata and analyze its characteristics. [Method] The full- length cDNA of SGD was cloned from R. verticillata with RACE technique. Then the expression levels in different tissues were analyzed with quantitative RT-PCR and the bioinformatic characteristics were also predicted. [Result] The full-length cDNA of RvSGD was 1 856 bp, containing a 1 608 bp CDS that encoded 536 amino acids with a calculated molecular mass of 61.0 kDa and an isoelectric point of 6.16. Bioinformatic analysis revealed that RvSGD shared high similarity with SGDs from Cantharanthus roseus and Rauvolfia serpentina at the amino acids; three conserved catalytic sites His-161, Glu-207 and Glu-419 were also presented in RvSGD. Quan- titative RT-PCR showed that expression level of RvSGD was the highest in barks, followed by old leaves, roots, tender leaves and tender stems. [Conclusion] The pre- sent study helps to understand more about the functions of the SGD gene at the level of molecular genetics, and provides new targets for molecular regulation of TIAs biosynthesis.

  10. Study on Roots of Firmiana plantanifolia (L.f.) Marsili

    Institute of Scientific and Technical Information of China (English)

    BAI,Hai-Yun; LI,Shuo; HU,Li-Hong

    2004-01-01

    @@ Firmiana plantanifolia (L.f.) Marsili is a Chinese medicine used in the treatment of numerous disorders such as rheumatism, asthma, fracture, and tumor etc.[1] In the course of a search for biological active compounds from medicinal plants,[2] we have examined the water extract of the root of this plant and succeeded in isolating three novel constituents named firmianones A, B and C (Scheme 1), along with 9 known compounds. Their UV, HRMS and NMR spectral data indicated that they are isoprenylated naphthoquinone dimmers. Firmianones A and B have a novel hexacyclo [14.8.0.02,7.06.15.099.14.018,23]tetracosa-1(16),3,9(14),10,12,7,19,21,23-nonaen-8-one skeleton and their absolute stereochemistry was determined by CD exciton-coupling experiments.

  11. Disturbances in LF radio-signals as seismic precursors

    Directory of Open Access Journals (Sweden)

    S. P. Kingsley

    2001-06-01

    Full Text Available Low Frequency (LF radio signals lie in the band 30-300 kHz. Monitoring equipment able to measure the electric strength of such signals, at field sites with very low noise levels, were designed and assembled in Italy. From 1993 onwards, the electric field strength of the MCO (216 kHz, France broadcasting station has been measured at two sites in Central Italy. At the end of 1996, radio signals from the CLT (189 kHz, Italy and the CZE (270 kHz, Czech Republic broadcasting stations were included in the measurements. During this monitoring period, evident attenuation of the electric field strength in some of the radio signals was observed at some of the receivers. The duration of the attenuation observed was several days and so it could have been related to particular meteorological conditions. On the other hand, this phenomenon could also represent precursors of moderate (3.0 =M =3.5 earthquakes that occurred near the receivers (within 50 km along the transmitter-receiver path. In this case it is possible that some local troposphere defocusing of the radio signals, produced by the pre-seismic processes, might have occurred. These observations were related only to moderate earthquakes and in these cases it may be that suitable meteorological conditions are needed to observe the effect. During February-March 1998 at one measuring site, we observed a significant increase in the CZE electric field strength. Unfortunately, the data of the other receiver could not be used in this case because of frequent interruptions in the recordings. The increase might have been a precursor of a strong earthquake (M = 5.3 that occurred on March 26, 1998 in the Umbria-Marche zone at a location over 100 km from the receiver, but which lay along the transmitter-receiver path. In this case, it is possible that an ionospheric disturbance, produced by the pre-seismic processes, might have occurred. If this pre-seismic behaviour of the LF signals could be confirmed then this

  12. Ex vivo intestinal adhesion of Escherichia coli LF82 in Crohn’s disease

    DEFF Research Database (Denmark)

    Jensen, Stina Rikke; Fink, Lisbeth Nielsen; Nielsen, Ole Haagen

    2011-01-01

    Adherent-invasive Escherichia coli (AIEC) are reported to inhabit the gut mucosa in Crohn’s disease (CD), however, little is known about the importance of host factors for the interplay between AIEC and the human gut.To examine if differences in bacterial adhesion patterns are disease associated......, the AIEC-prototype strain LF82 was evaluated for its ability to adhere to ileal and colonic biopsies from CD and healthy controls (HC). Moreover, the efficacy of the non-pathogenic E. coli Nissle 1917 (ECN) in averting LF82 adhesion to ileal mucosa was assessed.Similar numbers of LF82 adhered to biopsies...... from CD and HC. A significantly greater LF82 attachment to ileal versus colonic mucosa was found in HC (P adhesion of LF82 to ileal specimens in CD or HC.These results show that enhanced bacterial adhesion ability is unlikely to play any significant...

  13. The European VLF/LF Radio Network: the current status

    Science.gov (United States)

    Biagi, Pier Francesco; Maggipinto, Tommaso; Schiavulli, Luigi; Ligonzo, Teresa; Colella, Roberto; Ermini, Anita; Martinelli, Giovanni; Palangio, Paolo; Moldovan, Iren; Silva, Hugo; Contadakis, Michael; Frantzis, Xenophon; Katzis, Konstantinos; Buyuksarac, Aydin; D'Amico, Sebastiano

    2014-05-01

    Since 2009 a network of VLF (20-60 kHz) and LF (150-300 kHz) radio receivers has been put into operation in Europe in order to study earthquakes precursors. At the moment the network consists of eleven receivers four of which are located in Italy, two in Greece and one in Portugal, Romania, Malta, Cyprus and Turkey. The data (sampling rate of 1min) are downloaded automatically at the end of each day and they are stored in the server located at the Department of Physics of the University of Bari (Italy), that is the central node of the network. Still, in some case, problems of connection exist. The different trends are open and visible on the web site: http://beta.fisica.uniba.it/infrep/Hom.aspx. The data files can be downloaded by the same web site but they are protected by username and password. Among the different methods of data analysis the Wavelet spectra appear to be the most sensitive ones. The software able to apply this technique on the radio data automatically at the end of each day has been planned and realized. At the moment it operates on four signals collected by one of the Italian receivers; if an anomaly stands up and it is over a fixed threshold a warning advise appears. In the web site, this activity is protected by a specific username and password.

  14. Highly predictive support vector machine (SVM) models for anthrax toxin lethal factor (LF) inhibitors.

    Science.gov (United States)

    Zhang, Xia; Amin, Elizabeth Ambrose

    2016-01-01

    Anthrax is a highly lethal, acute infectious disease caused by the rod-shaped, Gram-positive bacterium Bacillus anthracis. The anthrax toxin lethal factor (LF), a zinc metalloprotease secreted by the bacilli, plays a key role in anthrax pathogenesis and is chiefly responsible for anthrax-related toxemia and host death, partly via inactivation of mitogen-activated protein kinase kinase (MAPKK) enzymes and consequent disruption of key cellular signaling pathways. Antibiotics such as fluoroquinolones are capable of clearing the bacilli but have no effect on LF-mediated toxemia; LF itself therefore remains the preferred target for toxin inactivation. However, currently no LF inhibitor is available on the market as a therapeutic, partly due to the insufficiency of existing LF inhibitor scaffolds in terms of efficacy, selectivity, and toxicity. In the current work, we present novel support vector machine (SVM) models with high prediction accuracy that are designed to rapidly identify potential novel, structurally diverse LF inhibitor chemical matter from compound libraries. These SVM models were trained and validated using 508 compounds with published LF biological activity data and 847 inactive compounds deposited in the Pub Chem BioAssay database. One model, M1, demonstrated particularly favorable selectivity toward highly active compounds by correctly predicting 39 (95.12%) out of 41 nanomolar-level LF inhibitors, 46 (93.88%) out of 49 inactives, and 844 (99.65%) out of 847 Pub Chem inactives in external, unbiased test sets. These models are expected to facilitate the prediction of LF inhibitory activity for existing molecules, as well as identification of novel potential LF inhibitors from large datasets.

  15. Synergistic Antimycobacterial Actions of Knowltonia vesicatoria (L.f Sims

    Directory of Open Access Journals (Sweden)

    Antoinette Labuschagné

    2012-01-01

    Full Text Available Euclea natalensis A.DC., Knowltonia vesicatoria (L.f Sims, and Pelargonium sidoides DC. are South African plants traditionally used to treat tuberculosis. Extracts from these plants were used in combination with isoniazid (INH to investigate the possibility of synergy with respect to antimycobacterial activity. The ethanol extract of K. vesicatoria was subjected to fractionation to identify the active compounds. The activity of the Knowltonia extract remained superior to the fractions with a minimum inhibitory concentration (MIC of 625.0 μg/mL against Mycobacterium smegmatis and an MIC of 50.00 μg/mL against M. tuberculosis. The K. vesicatoria extract was tested against two different drug-resistant strains of M. tuberculosis, which resulted in an MIC of 50.00 μg/mL on both strains. The combination of K. vesicatoria with INH exhibited the best synergistic antimycobacterial activity with a fractional inhibitory concentration index of 0.25 (a combined concentration of 6.28 μg/mL. A fifty percent inhibitory concentration of this combination against U937 cells was 121.0 μg/mL. Two compounds, stigmasta-5,23-dien-3-ol (1 and 5-(hydroxymethylfuran-2(5H-one (2, were isolated from K. vesicatoria as the first report of isolation for both compounds from this plant and the first report of antimycobacterial activity. Compound (1 was active against drug-sensitive M. tuberculosis with an MIC of 50.00 μg/mL.

  16. Karakteristik Bubuk Instan Cemcem ( spondiaz pinnata lf kurz

    Directory of Open Access Journals (Sweden)

    Luh Putu Wrasiati

    2015-04-01

    Full Text Available One of tropical plant used as a traditional beverage in Bali is Kecemcem or Cemcem (Spondias pinnata (Lf Kurz or in Indonesian called Kedondong Hutan. Empirically, the beverage is mentioned for treating coughs, increasing appetite, and body refreshment. Besides used as a beverage, Cemcem leafs are also used as a flavor enhancer of fish products. The study was carried out to determine the characteristics of Cemcem instant powder produced by encapsulation using maltodextrin and dried by the thin layer drying method. Cemcem instant powder was then analyzed its content of vitamin C, total phenols, tannins, antioxidant capacity, water cntent, ash content, and solubility. The flavor compounds in Cemcem instant powder obtained in Bukit Jimbaran area are identified by GC-MS devices. The result showed that cemcem instant powder which was produced by 24 hours of maceration time has the best characteristics and potential to be developed as a natural antioxidant products. It has the highest of total phenol, tannin, vitamin C, and antioxidant capacity. The content of vitamin C, total phenol, and tannin were 135.06 mg/100g, 38.95 mg GAE/g, and 11.01% respectively. The antioxidant activity of cemcem instant powder which analyzed by DPPH method was about 43.80%. There are 17 compounds detected by GC-MS, these compounds belonged alkenesalcoholsphenols compounds and esters of fatty acidsThe whole compounds were the building blocks of flavor cemcem leaves.Keywords : Characteristic of cemcem instant powder, antioxidant capacity, andflavor compounds of cemcem leaves

  17. Secoiridoids and other chemotaxonomically relevant compounds in Pedicularis: phytochemical analysis and comparison of Pedicularis rostratocapitata Crantz and Pedicularis verticillata L. from Dolomites.

    Science.gov (United States)

    Venditti, Alessandro; Frezza, Claudio; Sciubba, Fabio; Foddai, Sebastiano; Serafini, Mauro; Nicoletti, Marcello; Bianco, Armandodoriano

    2016-08-01

    We compared the respective metabolite patterns of two Pedicularis species from Dolomites. Seven phenylethanoid glycosides, i.e., verbascoside (1), echinacoside (2), angoroside A (3), cistantubuloside B1 (4), wiedemannioside C (5), campneoside II (11) and cistantubuloside C1 (12), together with several iridoid glucosides as aucubin (6), euphroside (7), monomelittoside (8), mussaenosidic acid (9) and 8-epiloganic acid (13) were identified. Pedicularis verticillata showed also the presence of greatly unexpected secoiridoids, ligustroside (14) and excelside B (15), very rare compounds in Lamiales. Both PhGs and iridoids are considered of taxonomical relevance in the Asteridae and their occurrence in Pedicularis was discussed. In particular, the exclusive presence of several compounds such as 8-epiloganic acid (13), campneoside II (11), cistantubuloside C1 (12), ligustroside (14) and excelside B (15) in Pedicularis rostratocapitata, and angoroside A (3), cistantubuloside B1 (4) and wiedemannioside C (5) in P. verticillata could be considered specific markers for the two botanical entities.

  18. Determinação de massa fresca, massa seca, água e cinzas totais de folhas de Cissus verticillata (L. Nicolson & C. E. Jarvis subsp. verticillata e avaliação do processo de secagem em estufa com ventilação forçada

    Directory of Open Access Journals (Sweden)

    T. V. BRAGA

    2009-01-01

    Full Text Available

    Cissus verticillata (L. Nicolson & C. E. Jarvis subsp. verticillata (Vitaceae é conhecida popularmente como insulina vegetal, cortina japonesa, uva-brava, anil trepador e cipó-pucá e utilizada na medicina popular na forma de chá das folhas no tratamento da diabetes, como antiinflamatório, antiepilético, antihipertensivo, antitérmico, antireumático, antigripal e contra infecções respiratórias. O objetivo deste estudo foi avaliar os teores de massa fresca, massa seca, percentual de água e percentual de cinzas totais, visando melhoria das condições de secagem, armazenamento, dispensação e uso pela população. As folhas da espécie foram coletadas no bairro Antônio Dias, Ouro Preto, Minas Gerais, Brasil. Foram selecionadas, lavadas e secas em estufa com ventilação forçada à 45ºC e determinou-se as cinzas totais e perda por dessecação (através do método gravimétrico, ambos de acordo com a Farmacopéia Brasileira. Os resultados mostraram que as folhas de C. verticillata subsp. verticillata possuem alto teor de água. O processo de secagem em estufa de ventilação forçada, a temperatura de 45ºC, foi eficaz, proporcionando folhas com 11,47% de umidade e 17,99% de cinzas totais. Palavras-chave: Cissus verticillata; insulina vegetal; qualidade; gravimetria.

  19. Possible earthquake precursors revealed by LF radio signals

    Directory of Open Access Journals (Sweden)

    P. F. Biagi

    2001-01-01

    Full Text Available Among radio signals, low frequency (LF radio signals lie in the band between 30–300 kHz. Monitoring equipment with the ability to measure the electric strength of such signals at field sites, were designed and assembled in Italy. From 1993 onwards, the electric field strength of the MCO (216 kHz, France broadcasting station has been collecting measurements at two sites in central Italy that were chosen according to very low noise levels. At the end of 1996, radio signals from the CLT (189 kHz, Italy and CZE (270 kHz, Czech Republic broadcasting stations were included in the measurements. Meteorological data from central Italy were also collected over the same time period in order to study the influence of weather conditions on the experimental measurements. During the monitoring period, we observed some evident attenuation of the electric field strength in some of the radio signals at some of the receivers. The duration of the attenuation observed was several days, so it could possibly be related to particular meteorological conditions. On the other hand, this phenomenon might represent precursors of moderate (3.0 M 3.5 earthquakes that occurred near the receivers (within 50 km along the transmitter-receiver path. In this case, it is possible that the pre-seismic processes could have produced irregularities in the troposphere, such as ducts, reflecting layers and scattering zones, so that some local troposphere defocusing of the radio signals might have occurred. These observations were related only to moderate earthquakes and in these cases, suitable meteorological conditions were probably needed to observe the effect. Between February – March 1998, we observed at one measuring site, a significant increase in the CZE electric field strength. Unfortunately, we could not use the data of the other receiver in this case, due to frequent interruptions in the data set. The increase might have been a precursor of the strong seismic sequence (M = 5

  20. Evaluation of the antimicrobial efficacy of Minthostachys verticillata essential oil and limonene against Streptococcus uberis strains isolated from bovine mastitis.

    Science.gov (United States)

    Montironi, Ivana D; Cariddi, Laura N; Reinoso, Elina B

    Bovine mastitis is a disease that causes great economic losses per year, being Streptococcus uberis the main environmental pathogen involved. The aim of the present study was to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of Minthostachys verticillata essential oil and limonene for S. uberis strains isolated from bovine mastitis. In addition, the effect of MIC on biofilm formation was analyzed. MIC values for the essential oil ranged from 14.3 to 114.5mg/ml (1.56-12.5%v/v) and MBC between 114.5 and 229mg/ml (12.5-25%v/v). MICs for limonene ranged from 3.3 to 52.5mg/ml (0.39-6.25%v/v) and MBC was 210mg/ml (25%v/v). Both compounds showed antibacterial activity and affected the biofilm formation of most of the strains tested. In conclusion, these compounds could be used as an alternative and/or complementary therapy for bovine mastitis caused by S. uberis. Copyright © 2016 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.

  1. PLC-δ1-Lf, a novel N-terminal extended phospholipase C-δ1.

    Science.gov (United States)

    Kim, Na Young; Ahn, Sang Jung; Kim, Moo-Sang; Seo, Jung Soo; Kim, Bo Seong; Bak, Hye Jin; Lee, Jin Young; Park, Myoung-Ae; Park, Ju Hyeon; Lee, Hyung Ho; Chung, Joon Ki

    2013-10-10

    Phospholipase C-δ (PLC-δ), a key enzyme in phosphoinositide turnover, is involved in a variety of physiological functions. The widely expressed PLC-δ1 isoform is the best characterized and the most well understood phospholipase family member. However, the functional and molecular mechanisms of PLC-δ1 remain obscure. Here, we identified that the N-terminal region of mouse PLC-δ1 gene has two variants, a novel alternative splicing form, named as long form (mPLC-δ1-Lf) and the previously reported short form (mPLC-δ1-Sf), having exon 2 and exon 1, respectively, while both the gene variants share exons 3-16 for RNA transcription. Furthermore, the expression, identification and enzymatic characterization of the two types of PLC-δ1 genes were compared. Expression of mPLC-δ1-Lf was found to be tissue specific, whereas mPLC-δ1-Sf was widely distributed. The recombinant mPLC-δ1-Sf protein exhibited higher activity than recombinant mPLC-δ1-Lf protein. Although, the general catalytic and regulatory properties of mPLC-δ1-Lf are similar to those of PLC-δ1-Sf isozyme, the mPLC-δ1-Lf showed some distinct regulatory properties, such as tissue-specific expression and lipid binding specificity, particularly for phosphatidylserine.

  2. Brukarens roll i välfärdsforskning och utvecklingsarbete

    OpenAIRE

    Ander, Birgitta; Bennich, Maria; Bolling, Jamie; Bülow, Pia; Cedersund, Elisabet; Eriksson, Bengt.G.; Felizia, Inga-Lill; Granerud, Arild; Hanson, Elizabeth; Hummelvoll, Jan Kåre; Jonsson, Oskar; Karlsson, Per-Åke; Kogstad, Ragnfrid Eline; Käcker, Pia; Magnusson, Lennart

    2011-01-01

    Fra omslag: På 1980-talet blev ”brukare” ett modeord i offentlig förvaltning och förvaltningsforskning. Termen betecknar den som använder sig av välfärdsservice (jfr. engelskans service user), eller ”slutmottagare” av offentlig nyttighet eller åtgärd. Brukare av välfärdstjänster vet hur hjälp och service fungerar i praktiken och kan därför ge synnerligen viktig återkoppling enligt devisen: ”Den som har skorna på fötterna vet var de skaver”. Välfärdsorganisationer har all anledn...

  3. Ileal adhesion of virulent E. coli LF82 is not enhanced in Crohn’s disease

    DEFF Research Database (Denmark)

    Jensen, Rikke S.; Fink, Lisbeth Nielsen; Pedersen, Susanne Brix

    2011-01-01

    of CD has been further strengthened from the evidence that the ileum in CD harbors an abnormally high number of E. coli species. S16 2010 IBD Abstracts The aim of this study was to examine the adhesion of the AIEC reference strain, LF82, to tissue samples from ileum and colon in CD and healthy controls....... A second purpose was to assess the probiotic efficacy of E. coli Nissle 1917 (ECN) in averting LF82 adhesion to ileal mucosa. Ileal and colonic specimens were obtained from patients with CD ileitis and controls (n¼10). A model was developed to investigate bacterial adhesion to intestinal biopsies...... and employed for investigation of the bacterial adherence to human intestinal specimens. LF82 adhered to intestinal biopsies in both CD and controls. Enhanced adhesion was, however, not observed in the ileum as compared to the colon in CD, which was in contradiction to controls that had a significantly higher...

  4. Ileal adhesion of virulent E. coli LF82 is not enhanced in Crohn’s disease

    DEFF Research Database (Denmark)

    Jensen, Rikke S.; Fink, Lisbeth Nielsen; Pedersen, Susanne Brix

    2011-01-01

    of CD has been further strengthened from the evidence that the ileum in CD harbors an abnormally high number of E. coli species. S16 2010 IBD Abstracts The aim of this study was to examine the adhesion of the AIEC reference strain, LF82, to tissue samples from ileum and colon in CD and healthy controls...... and employed for investigation of the bacterial adherence to human intestinal specimens. LF82 adhered to intestinal biopsies in both CD and controls. Enhanced adhesion was, however, not observed in the ileum as compared to the colon in CD, which was in contradiction to controls that had a significantly higher...

  5. Anthrax lethal factor (LF) mediated block of the anthrax protective antigen (PA) ion channel: effect of ionic strength and voltage.

    Science.gov (United States)

    Neumeyer, Tobias; Tonello, Fiorella; Dal Molin, Federica; Schiffler, Bettina; Orlik, Frank; Benz, Roland

    2006-03-07

    The anthrax toxin complex consists of three different molecules, protective antigen (PA), lethal factor (LF), and edema factor (EF). The activated form of PA, PA(63), forms heptamers that insert at low pH in biological membranes forming ion channels and that are necessary to translocate EF and LF in the cell cytosol. LF and EF are intracellular active enzymes that inhibit the host immune system promoting bacterial outgrowth. Here, PA(63) was reconstituted into artificial lipid bilayer membranes and formed ion-permeable channels. The heptameric PA(63) channel contains a binding site for LF on the cis side of the channel. Full-size LF was found to block the PA(63) channel in a dose- and ionic-strength-dependent way with half-saturation constants in the nanomolar concentration range. The binding curves suggest a 1:1 relationship between (PA(63))(7) and bound LF that blocks the channel. The presence of a His(6) tag at the N-terminal end of LF strongly increases the affinity of LF toward the PA(63) channel, indicating that the interaction between LF and the PA(63) channel occurs at the N terminus of the enzyme. The LF-mediated block of the PA(63)-induced membrane conductance is highly asymmetric with respect to the sign of the applied transmembrane potential. The result suggested that the PA(63) heptamers contain a high-affinity binding site for LF inside domain 1 or the channel vestibule and that the binding is ionic-strength-dependent.

  6. Characteristics of VLF/LF Sferics from Elve-producing Lightning Discharges

    Science.gov (United States)

    Blaes, P.; Zoghzoghy, F. G.; Marshall, R. A.

    2013-12-01

    Lightning return strokes radiate an electromagnetic pulse (EMP) which interacts with the D-region ionosphere; the largest EMPs produce new ionization, heating, and optical emissions known as elves. Elves are at least six times more common than sprites and other transient luminous events. Though the probability that a lightning return stroke will produce an elve is correlated with the return stroke peak current, many large peak current strokes do not produce visible elves. Apart from the lightning peak current, elve production may depend on the return stroke speed, lightning altitude, and ionospheric conditions. In this work we investigate the detailed structure of lightning that gives rise to elves by analyzing the characteristics of VLF/LF lightning sferics in conjunction with optical elve observations. Lightning sferics were observed using an array of six VLF/LF receivers (1 MHz sample-rate) in Oklahoma, and elves were observed using two high-speed photometers pointed over the Oklahoma region: one located at Langmuir Laboratory, NM and the other at McDonald Observatory, TX. Hundreds of elves with coincident LF sferics were observed during the summer months of 2013. We present data comparing the characteristics of elve-producing and non-elve producing lightning as measured by LF sferics. In addition, we compare these sferic and elve observations with FDTD simulations to determine key properties of elve-producing lightning.

  7. Studies on the Constituents of Ficus microcarpa L.f. 1. Triterpenoids from the Leaves

    OpenAIRE

    Higa, Matsutake; Yogi, Seiichi; Hokama, Kozo; 比嘉, 松武; 与儀, 誠一; 外間, 宏三

    1987-01-01

    The following triterpenoids were isolated from the ethanol extracts of the leaves of Ficus microcarpa L.f., together with aliphatic compounds and steroids: lupenyl acetate, friedelin, glutinol, epifriedelinol, taraxerol and oleanolic acid. Two unknown pentacyclic triterpenoids were also isolated and their structures were discussed from spectroscopic evidences.

  8. 1047-nm all-solid-state laser based on Nd: LuLF

    Institute of Scientific and Technical Information of China (English)

    Rui Li; Ting Yu; Lianhan Zhang; Weibiao Chen; Yin Hang

    2011-01-01

    @@ A compact all-solid-state continuous-wave (CW) laser at 1047 nm is developed based on Nd:LuLF, which is grown through the Czochralski technique. From the laser system, 1.3-W laser can be obtained, which corresponds to the slope efficiencies of 20.1% and 49.5% with respect to the incident and absorbed pump powers, respectively. To the best of our knowledge, this is the highest power level achieved at 1047 nm based on the Nd:LuLF crystal.%A compact all-solid-state continuous-wave (CW) laser at 1047 nm is developed based on Nd:LuLF, which is grown through the Czochralski technique. From the laser system, 1.3-W laser can be obtained, which corresponds to the slope efficiencies of 20.1% and 49.5% with respect to the incident and absorbed pump powers, respectively. To the best of our knowledge, this is the highest power level achieved at 1047 nm based on the Nd:LuLF crystal.

  9. The LF of TP-AGB stars in the LMC/SMC

    Science.gov (United States)

    Bruzual, Gustavo; Charlot, Stephane; GonzalezLopezlira, Rosa; Srinivasan, Sundar; Boyer, Martha L.

    2013-01-01

    We show that Monte Carlo simulations of the TP-AGB stellar population in the LMC and SMC galaxies using the CB. models produce LF and color distributions that are in closer agreement with observations than those obtained with the BC03 and CB07 models. This is a progress report of work that will be published elsewhere.

  10. Demonstration of a hybrid Ho:YLF Ho:LuLF slab laser

    CSIR Research Space (South Africa)

    Esser, MJD

    2010-10-01

    Full Text Available The authors present a continuous-wave slab laser utilising both Ho:YLF and Ho:LuLF as laser gain media. 30 W of output power at 2 µm was demonstrated in a stable concave-plane resonator while 13 W was achieved in a hybrid stable...

  11. LFTOP: An LF-Based Approach to Domain-Specific Reasoning

    Institute of Scientific and Technical Information of China (English)

    Jian-Min Pang; Paul Callaghan; Zhao-Hui Luo

    2005-01-01

    A new approach to domain-specific reasoning is presented that is based on a type-theoretic logical framework (LF) but does not require the user to be an expert in type theory. The concepts of the domain and its related reasoning systems are formalized in LF, but the user works with the system through a syntax and interface appropriate to his/her work. A middle layer provides translation between the user syntax and LF, and allows additional support for reasoning (e.g., model checking). Thus, the complexity of the logical framework is hidden but the benefits of using type theory and its related tools are retained, such as precision and machine-checkable proofs. This approach is investigated through a number of case studies: here, the authors consider the verification of properties of concurrency. The authors have formalized a specification language (CCS) and logic (μ-calculus) in LF, together with useful lemmas, and a user-oriented syntax has been designed. The authors demonstrate the approach with simple examples. However, applying lemmas to objects introduced by the user may result in framework-level objects which cannot be translated back to the user level. The authors discuss this problem, define a notion of adequacy, and prove that in this case study, translation can always be reversed.

  12. 萝芙木中化学成分的研究%Chemical constituents of Rauvolfia verticillata

    Institute of Scientific and Technical Information of China (English)

    洪博; 李文静; 赵春杰

    2012-01-01

    为研究夹竹桃科植物萝芙术的化学成分及药理活性,通过硅胶、凝胶LH-20、反相开口柱等色谱方法进行分离纯化.根据化合物的理化性质和波谱数据鉴定化学结构,从氯仿层提取物中分离得到3个吲哚类生物碱和1个吖啶酮类生物碱,分别鉴定为萝芙碱B(1)、山德维辛碱(2)、萝尼生(3)和7—羟基—吖啶酮(4).化合物1为新化合物,属于吲哚类生物碱.化合物4是吖啶酮类生物碱,为首次从萝芙木属植物中分离得到的化合物类型.本文对化合物4这一新类型的生物碱做了生物活性研究,以发掘萝芙木除降压以外其他的药理活性.%The study on the Rauvolfia verticillata (Lour.) Baill., which belongs to Apocynaeeae, was carried out to look for its chemical constituents and pharmacological activity. The isolation and purification were performed by chromatography on silica gel, Sephadex LH-20 and ODS (octadecyl silane) open column. The structures of obtained compounds were elucidated on the basis of physicochemical properties and spectral analysis. Three indole alkaloids and one acridone alkaloid were isolated from chloroform layer extract and identified as ajmalicine B (1), sandwicine (2), raunescine (3) and 7-hydroxynoracronycine (4) separately. Ajmalicine B (1) is a new compound belonging to indole alkaloid. Compound 4 as an acridone alkaloid was a new type compound isolated from Rauvolfia genus for the first time. We also did some biological activity research on the new type compound (4) to explore other pharmacological activities in addition to antihypertensive activity.

  13. Prediction Capabilities of VLF/LF Emission as the Main Precursor of Earthquake

    CERN Document Server

    Kachakhidze, Manana

    2013-01-01

    Recent satellite and ground-based observations proved that in earthquake preparation period in the seismogenic area we have VLF/LF and ULF electromagnetic emissions. According to the opinion of the authors of the present paper this phenomenon is more universal and reliable than other earthquake indicators. Hypothetically, in case of availability of adequate methodological grounds, in the nearest future, earth VLF/LF electromagnetic emission might be declared as the main precursor of earthquake. In particular, permanent monitoring of frequency spectrum of earth electromagnetic emission generated in the earthquake preparation period might turn out very useful with the view of prediction of large (M 5) inland earthquakes. The present paper offers a scheme of the methodology according to which the reality of the above given hypothesis can be checked up. To prove the prediction capabilities of earth electromagnetic emission we have used avalanche-like unstable model of fault formation and an analogous model of ele...

  14. Dimensional instability of LF21 aluminum alloy weldments at room temperature and after thermal cycles

    Institute of Scientific and Technical Information of China (English)

    刘雪松; 田锡唐; 徐文立

    2002-01-01

    The unstable dimensional distortion of LF21 aluminum alloy weldments at room temperature and after thermal cycles was studied by use of light interference and CMM. At the same time, distortion mechanism was analyzed from the viewpoint of mechanics and microstructure. Experimental results show that there exists obvious difference of unstable dimensional distortion between LF21 welded specimens under two conditions mentioned above. Under room temperature, dimensional variation of welded specimens will decrease gradually and finally tends to be stable during 130 h after welding. The relative elongation of welded specimen is 4.2×10-5. After thermal cycles, distortion of welded specimen is much larger than that at room temperature. After 11 thermal cycles, the dimension will tend to be stable. Dimensional unstable distortion of weldments mainly results from temperature condition, microstructure variation and relaxation of welding residual stress.

  15. Reception conditions of low frequency (LF) transmitter signals onboard DEMETER micro-satellite

    Science.gov (United States)

    Boudjada, Mohammed Y.; Biagi, Pier F.; Sawas, Sami; Galopeau, Patrick H. M.; Besser, Bruno; Wolbang, Daniel; Prattes, Gustav; Eichelberger, Hans; Stangl, Günter; Parrot, Michel; Schwingenschuh, Konrad

    2014-05-01

    We analyze the flux density variation associated to low frequency (LF) broadcasting transmitters observed by the ICE electric field experiment onboard DEMETER micro-satellite. We select five stations localised around the Mediterranean and the black seas: Tipaza (252 kHz, 02°28'E, 36°33'N, Algeria), Roumoules (216 kHz, 06°08'E, 43°47'N, Monte Carlo), Polatli (180 kHz, 32°25'E, 39°45'N, Turkey), Nador (171 kHz, 02°55'W, 35°02'N, Morocco) and Brasov (153 kHz, 25°36'E,45°40', Romania). The detection of the LF transmitter signals by DEMETER micro-satellite is found to depend on the radiated power, the emitted frequency, and the orbit paths with regard to the location of the stations. This leads us to characterise the reception condition of the LF signals and to define time intervals where the detection probability is high. We firstly discuss the dependence of the reception conditions on the ionospheric disturbances due to the geomagnetic and solar activities, and we secondly attempt to estimate the global electric environment above the Mediterranean and the black seas.

  16. Hybrid Modeling for Soft Sensing of Molten Steel Temperature in LF

    Institute of Scientific and Technical Information of China (English)

    TIAN Hui-xin; MAO Zhi-zhong; WANG An-na

    2009-01-01

    Aiming at the limitations of traditional thermal model and intelligent model, a new hybrid model is established for soft sensing of the molten steel temperature in LF. Firstly, a thermal model based on energy conservation is described; and then, an improved intelligent model based on process data is presented by ensemble ELM (extreme learning machine) for predicting the molten steel temperature in LF. Secondly, the self-adaptive data fusion is proposed as a hybrid modeling method to combine the thermal model with the intelligent model. The new hybrid model could complement mutual advantage of two models by combination. It can overcome the shortcoming of parameters obtained on-line hardly in a thermal model and the disadvantage of lacking the analysis of ladle furnace metallurgical process in an intelligent model. The new hybrid model is applied to a 300 t LF in Baoshan Iron and Steel Co Ltd for predicting the molten steel temperature. The experiments demonstrate that the hybrid model has good generalization performance and high accuracy.

  17. Pollen dispersal in fragmented populations of the dioecious wind-pollinated tree, Allocasuarina verticillata (drooping sheoak, drooping she-oak; Allocasuarinaceae.

    Directory of Open Access Journals (Sweden)

    Linda Broadhurst

    Full Text Available Vegetation clearing, land modification and agricultural intensification have impacted on many ecological communities around the world. Understanding how species respond to fragmentation and the scales over which functionality is retained, can be critical for managing biodiversity in agricultural landscapes. Allocasuarina verticillata (drooping sheoak, drooping she-oak is a dioecious, wind-pollinated and -dispersed species with key conservation values across southeastern Australia. But vegetation clearing associated with agricultural expansion has reduced the abundance and spatial distribution of this species in many regions. Spatial genetic structure, relatedness among trees, pollen dispersal and mating patterns were examined in fragmented A. verticillata populations selected to represent the types of remnants that now characterise this species. Short scale spatial genetic structure (5-25 m and relatedness among trees were observed in most populations. Unexpectedly, the two male trees closest to each female did not have a reproductive advantage accounting for only 4-15% of the seed produced in larger populations. Biparental inbreeding was also generally low (<4% with limited evidence of seed crop domination by some male trees. More male trees contributed to seed crops in linear remnants (mean 17 compared to those from patch remnants (mean 11.3 which may reflect differences in pollen dispersal within the two remnant types. On average, pollen travels ~100 m irrespective of remnant type but was also detected to have dispersed as far as 1 km in open landscapes. Low biparental inbreeding, limited reproductive assurance for near-neighbour and probably related males and variability in the distances over which females sample pollen pools suggest that some mechanism to prevent matings between relatives exists in this species.

  18. Adherent-invasive Escherichia coli, strain LF82 disrupts apical junctional complexes in polarized epithelia

    Directory of Open Access Journals (Sweden)

    Ossa Juan C

    2009-08-01

    Full Text Available Abstract Background Although bacteria are implicated in the pathogenesis of chronic inflammatory bowel diseases (IBD, mechanisms of intestinal injury and immune activation remain unclear. Identification of adherent-invasive Escherichia coli (AIEC strains in IBD patients offers an opportunity to characterize the pathogenesis of microbial-induced intestinal inflammation in IBD. Previous studies have focused on the invasive phenotype of AIEC and the ability to replicate and survive in phagocytes. However, the precise mechanisms by which these newly identified microbes penetrate the epithelial lining remain to be clarified. Therefore, the aim of this study was to delineate the effects of AIEC, strain LF82 (serotype O83:H1 on model polarized epithelial monolayers as a contributor to intestinal injury in IBD. Results Infection of T84 and Madin-Darby Canine Kidney-I polarized epithelial cell monolayers with AIEC, strain LF82 led to a reduction in transepithelial electrical resistance and increased macromolecular (10 kilodalton dextran flux. Basolateral AIEC infection resulted in more severe disruption of the epithelial barrier. Increased permeability was accompanied by a redistribution of the tight junction adaptor protein, zonula occludens-1, demonstrated by confocal microscopy and formation of gaps between cells, as shown by transmission electron microscopy. After 4 h of infection of intestine 407 cells, bacteria replicated in the cell cytoplasm and were enclosed in membrane-bound vesicles positive for the late endosomal marker, LAMP1. Conclusion These findings indicate that AIEC, strain LF82 disrupts the integrity of the polarized epithelial cell barrier. This disruption enables bacteria to penetrate into the epithelium and replicate in the host cell cytoplasm. These findings provide important links between microbes related to IBD, the intestinal epithelial cell barrier and disease pathogenesis.

  19. Micropropagación de teca (Tectona grandis L.f)

    OpenAIRE

    Abdelnour-Esquivel, Ana; Instituto Tecnológico de Costa Rica, Escuela de Ingeniería Forestal; Cartago, Costa Rica; Muñoz, Anaballe; Instituto Tecnológico de Costa Rica, Escuela de Ingeniería Forestal; Cartago, Costa Rica

    2012-01-01

    La teca (Tectona grandis L.f.) es una especie considerada valiosa por la calidad de su madera. La alta demanda a nivel mundial ha impulsado su cultivo en plantaciones comerciales. Sin embargo, la calidad de muchas de estas plantaciones justificó el inicio de programas de mejoramiento de la especie. La introducción de técnicas de micropropagación o propagación clonal in vitro (en condiciones de laboratorio) en los programas de mejoramiento genético y para el establecimiento de plantaciones con...

  20. Electron density profiles in the background of LF absorption during Forbush-decrease and PSE

    Science.gov (United States)

    Satori, G.

    1989-01-01

    Based on the simulation of different Forbush decrease and particle precipitation effects in the D region, electron density profiles in the mid-latitudes the ionospheric absorption of low frequency (LF) radio waves was determined. The absorption variations at different frequenceis are strongly affected by the shape of the electron density profile. A structure appears which sometimes resembles the letter S (in a sloping form). Both the height (around 70 to 72 km) and the depth of the local minimum in the electron density contribute to the computed absorption changes of various degree at different frequencies. In this way several observed special absorption events can be interpreted.

  1. Digital control of pulsed gas metal arc welding inverter using TMS320LF2407A

    Institute of Scientific and Technical Information of China (English)

    Wu Kaiyuan; Huang Shisheng; Li Xinglin; Wu Shuifeng

    2008-01-01

    A digital control of pulsed gas metal arc welding inverter was proposed. A control system consisting of analogue parts was replaced with a new digital control implemented in a TMS320LF2407A DSP chip. The design and constructional features of the whole digital control were presented. The resources of the DSP chip were efficiently utilized and the circuits are very concise, which can enhance the stability and reliability of welding inverter. Experimental results demonstrate that the developed digital control has the ability to accomplish the excellent pulsed gas metal arc welding process and the merits of the developed digital control are stable welding process, little spatter and perfect weld appearance.

  2. A novel homogenization procedure to model the skin layers in LF numerical dosimetry

    Science.gov (United States)

    De Santis, Valerio; Chen, Xi Lin; Cruciani, Silvano; Campi, Tommaso; Feliziani, Mauro

    2016-06-01

    In this study we focus on the validity of the skin layer currently implemented in up-to-date human-body anatomical models employed in low frequency (LF) numerical dosimetry. Indeed, the several layers of the skin structure, i.e. the stratum corneum (SC), dermis, and epidermis are in these models embedded into a unique fairly-thick (2-3 mm) layer encompassing all of them. While a previous work from the authors showed that for normal-standing (or limb-non-touching) postures a single-layer skin model could conservatively estimate the peak electric field induced in the skin, at least a two-layer skin model comprising of the SC and the remaining skin layers should be used for limb-touching exposure scenarios. This implies notable efforts to discretize the tiny SC layer questioning the validity of current anatomical models. A novel strategy based on the homogenization of the several skin layers has been therefore proposed in order to eliminate the SC from the computational domain opening the doors to future LF magnetic applications even for limb-touching scenarios.

  3. The Lectin Frontier Database (LfDB, and Data Generation Based on Frontal Affinity Chromatography

    Directory of Open Access Journals (Sweden)

    Jun Hirabayashi

    2015-01-01

    Full Text Available Lectins are a large group of carbohydrate-binding proteins, having been shown to comprise at least 48 protein scaffolds or protein family entries. They occur ubiquitously in living organisms—from humans to microorganisms, including viruses—and while their functions are yet to be fully elucidated, their main underlying actions are thought to mediate cell-cell and cell-glycoconjugate interactions, which play important roles in an extensive range of biological processes. The basic feature of each lectin’s function resides in its specific sugar-binding properties. In this regard, it is beneficial for researchers to have access to fundamental information about the detailed oligosaccharide specificities of diverse lectins. In this review, the authors describe a publicly available lectin database named “Lectin frontier DataBase (LfDB”, which undertakes the continuous publication and updating of comprehensive data for lectin-standard oligosaccharide interactions in terms of dissociation constants (Kd’s. For Kd determination, an advanced system of frontal affinity chromatography (FAC is used, with which quantitative datasets of interactions between immobilized lectins and >100 fluorescently labeled standard glycans have been generated. The FAC system is unique in its clear principle, simple procedure and high sensitivity, with an increasing number (>67 of associated publications that attest to its reliability. Thus, LfDB, is expected to play an essential role in lectin research, not only in basic but also in applied fields of glycoscience.

  4. A comparative study of measured amplitude and phase perturbations of VLF and LF radio signals induced by solar flares

    Directory of Open Access Journals (Sweden)

    Šulić D.M.

    2014-01-01

    Full Text Available Very Low Frequency (VLF and Low Frequency (LF signal perturbations were examined to study ionospheric disturbances induced by solar X-ray flares in order to understand processes involved in propagation of VLF/LF radio signals over short paths and to estimate specific characteristics of each short path. The receiver at the Belgrade station is constantly monitoring the amplitude and phase of a coherent and subionospherically propagating LF signal operated in Sicily NSC at 45.90 kHz, and a VLF signal operated in Isola di Tavolara ICV at 20.27 kHz, with the great circle distances of 953 km and 976 km, respectively. A significant number of similarities between these short paths is a direct result of both transmitters and the receiver’s geographic location. The main difference is in transmitter frequencies. From July 2008 to February 2014 there were about 200 events that were chosen for further examination. All selected examples showed that the amplitude and phase of VLF and LF signals were perturbed by solar X-ray flares occurrence. This six-year period covers both minimum and maximum of solar activity. Simultaneous measurement of amplitude and phase of the VLF/LF signals during a solar flare occurrence was applied to evaluate the electron density profile versus altitude, to carry out the function of time over the middle Europe. [Projekat Ministarstva nauke Republike Srbije, br. 176002 i br. III4402

  5. Biochemical fingerprint and pharmacological applications of Barleria noctiflora L.f. leaves.

    Science.gov (United States)

    Alagar Yadav, Sangilimuthu; Ramalingam, Sathishkumar; Jebamalairaj, Anitha; Subban, Ravi; Sundaram, Karpagavalli Mennakshi

    2016-12-01

    Background Antioxidant and antihistamine agents from Barleria noctiflora L.f. as natural source due to the existing modern medicine give various adverse effects to overcome these problems with natural products. MethodsB. noctiflora leaves extract was fractionated with column chromatography; the homogenized fractions were monitored with thin layer chromatography (TLC) and characterized by using UV-visible, FT-IR, 1H NMR, 13C NMR and mass spectrometry spectral studies. The volatile phytoconstituents of B. noctiflora extract were analysed by gas chromatography-mass spectrometry. Phytoconstituents from B. noctiflora leaves extract were screened for their antioxidant and antihistamine potential in vitro (2,2-diphenyl-1-picrylhydrazyl radical scavenging activity, 2,2'-azinobis-3-ethylbenzothiozoline-6-sulfonic acid radical decolouration assay, nitric oxide radical scavenging activity, superoxide radical scavenging activity and hydrogen peroxide radical scavenging activity) and in silico (molecular docking), respectively. Results Antioxidant and antihistamine barlerinoside has been isolated and characterized from the leaves of B. noctiflora L.f. Barlerinoside revealed their free-radical scavenging ability on OH-, OH•, NO-, O2- and H2O2 radicals and found high percentage inhibition against OH- radical at the IC50 value of 50.45±2.52  µg. The methanol (MeOH) extract of B. noctiflora leaves contains cyclotene; N,N-dimethylglycine; tetrahydrocyclopenta [1,3] dioxin-4-one; phenol, 2-methoxy-; benzofuran, 2-methyl-; 1,4:3,6-dianhydro-α-d-glucopyranose; 2-methoxy-4-vinylphenol; 1,3;2,5-dimethylene-l-rhamnitol; levoglucosan and bicyclo[2.2.2]oct-7-ene-2,5-dione as being the major compounds. Among phytoconstituents present in the extract, the hexestrol; 1,2-benzenedicarboxylic acid, bis(2-methylpropyl) ester; 1-(3,6,6-trimethyl-1,6,7,7a-tetrahydrocyclopenta[c]pyran-1-yl) ethanone; megastigmatrienone; furan interacted with histamine H1 receptor and bind at GLU-177 and ASP

  6. Amelioration of Colitis in Mouse Model by Exploring Antioxidative Potentials of an Indigenous Probiotic Strain of Lactobacillus fermentum Lf1

    Directory of Open Access Journals (Sweden)

    Ritu Chauhan

    2014-01-01

    Full Text Available Based on the preliminary screening of eight indigenous putative probiotic Lactobacilli, Lactobacillus fermentum Lf1 was selected for assessing its antioxidative efficacy in DSS colitis mouse model based on its ability to enhance the expression of “Nrf2” by 6.43-fold and malondialdehyde (MDA inhibition by 78.1  ±  0.24% in HT-29 cells under H2O2 stress. The Disease Activity Index and histological scores of Lf1-treated mice were lower than the control group. However, expression of “Nrf2” was not observed in Lf1-treated mice. A significant increase in the expression of antioxidative enzymes such as SOD2 and TrxR-1 was recorded in both of the groups. The expression of SOD2 was significantly downregulated in colitis-induced mice by −100.00-fold relative to control group, and the downregulation was considerably reduced to −37.04-fold in colitis Lf1 treatment group. Almost, a similar trend was recorded in case of “thioredoxin” expression, though “CAT” was refractile to expression. The Lf1-treated group had decreased malondialdehyde level as compared to colitis control (37.92  ±  6.31 versus 91.13  ±  5.76 μM/g. These results point towards Lf1-induced activation of the antioxidant enzyme system in the mouse model and its prospects to be explored as a new strategy for IBD management.

  7. Influence of Pressure on SiNx:H Film by LF-PECVD

    Institute of Scientific and Technical Information of China (English)

    Zhen-li Wen; Xiao-ning Cao; Chun-lan Zhou; Wen-jing Wang

    2012-01-01

    Hydrogenated silicon nitride films as an effective antireflection and passivation coating of silicon solar cell were prepared on p-type polished silicon substrate (1.0 Ωcm) by direct LF-PECVD (low frequency plasma enhanced chemical vapor deposition) of Centrotherm.The preferable passivation effect was obtained and the refractive index was in the range of 2.017-2.082.The refractive index of the hydrogenated silicon nitride films became larger with the increase of the pressure. Fourier transform infrared spectroscopy was used to study the pressure influence on the film structural properties.The results highlighted high hydrogen bond and high Si-N bonds density in the film,which were greatly influenced by the pressure.The passivation effect of the films was influenced by the Si dangling bonds density.Finally the effective minority liftetime degradation with time was shown and discussed by considering the relationship between the structural properties and passivation.

  8. Hjólför í íslensku malbiki. Slit og deigar formbreytingar

    OpenAIRE

    Birkir Hrafn Jóakimsson 1981

    2014-01-01

    Hjólfaramyndun er umtalsvert vandamál vegum og götum hér á landi. Hjólför myndast vegna slits annars vegar og deigra formbreytinga hins vegar. Slit má rekja til umferðar bifreiða búnum negldum hjólbörðum og formbreytingar, skrið aðallega, má tengja við umferð þungra ökutækja. Þetta verkefni fjallar um hvernig hægt er að spá fyrir um hjólfaramyndun í malbiki bæði vegna slits og deigra formbreytinga. Á Íslandi hefur hingað til ekki verið hægt að spá fyrir um hjólfaramyndun vegna nagldekkja. ...

  9. Exopolysaccharide from Lactobacillus fermentum Lf2 and its functional characterization as a yogurt additive.

    Science.gov (United States)

    Ale, Elisa C; Perezlindo, Marcos J; Burns, Patricia; Tabacman, Eduardo; Reinheimer, Jorge A; Binetti, Ana G

    2016-11-01

    Lactobacillus fermentum Lf2 is a strain which is able to produce high levels (approximately 1 g/l) of crude exopolysaccharide (EPS) when it is grown in optimised conditions. The aim of this work was to characterize the functional aspects of this EPS extract, focusing on its application as a dairy food additive. Our findings are consistent with an EPS extract that acts as moderate immunomodulator, modifying s-IgA and IL-6 levels in the small intestine when added to yogurt and milk, respectively. Furthermore, this EPS extract, in a dose feasible to use as a food additive, provides protection against Salmonella infection in a murine model, thus representing a mode of action to elicit positive health benefits. Besides, it contributes to the rheological characteristics of yogurt, and could function as a food additive with both technological and functional roles, making possible the production of a new functional yogurt with improved texture.

  10. Effect of the total solar eclipse of March 20, 2015, on VLF/LF propagation

    Science.gov (United States)

    Solovieva, M. S.; Rozhnoi, A. A.; Fedun, V.; Schwingenschuh, K.

    2016-05-01

    The analyzed amplitude and phase variations in electromagnetic VLF and LF signals at 20-45 kHz, received in Moscow, Graz (Austria), and Sheffield (UK) during the total solar eclipse of March 20, 2015, are considered. The 22 analyzed paths have lengths of 200—6100 km, are differently oriented, and cross 40-100% occultation regions. Fifteen paths crossed the region where the occultation varied from 40 to 90%. Solar eclipse effects were found only on one of these paths in the signal phase (-50°). Four long paths crossed the 90-100% occultation region, and signal amplitude and phase anomalies were detected for all four paths. Negative phase anomalies varied from-75° to-90°, and the amplitude anomalies were both positive and negative and were not larger than 5 dB. It was shown that the effective height of the ionosphere varied from 6.5 to 11 km during the eclipse.

  11. Mauritic acid: a new dammarane triterpene from the roots of Mauritia flexuosa L.f. (Arecaceae).

    Science.gov (United States)

    Koolen, Hector H F; Soares, Elzalina R; da Silva, Felipe M A; de Oliveira, Aimêe A; de Souza, Antonia Q L; de Medeiros, Lívia S; Rodrigues-Filho, Edson; Cavalcanti, Bruno C; Pessoa, Cláudia O; Moraes, Manoel O; Salvador, Marcos J; de Souza, Afonso D L

    2013-01-01

    A new dammarane triterpene named mauritic acid (1) was isolated from the roots of Mauritia flexuosa L.f. The complete structural assignment of this new compound was elucidated from spectroscopic methods. Moreover, this compound was evaluated for its cytotoxicity against human cancer cell lines (OVCAR-8, PCM3, NCIH358M and different leukaemia cell strains). The mauritic acid presented significant cytotoxicity against OVCAR-8, PCM3 and NCIH358M cell lines with IC50 3.02, 2.39 and 6.19 μM, respectively. The triterpenes 1 and 2 were also tested for their antimicrobial activity against 15 strains of microorganisms, including fungi and bacteria, with the best minimal inhibitory concentration values ranging from 50.8 to 203.5 μM.

  12. Gluing together Proof Environments: Canonical extensions of LF Type Theories featuring Locks

    Directory of Open Access Journals (Sweden)

    Furio Honsell

    2015-07-01

    Full Text Available We present two extensions of the LF Constructive Type Theory featuring monadic locks. A lock is a monadic type construct that captures the effect of an external call to an oracle. Such calls are the basic tool for gluing together diverse Type Theories and proof development environments. The oracle can be invoked either to check that a constraint holds or to provide a suitable witness. The systems are presented in the canonical style developed by the CMU School. The first system, CLLFP, is the canonical version of the system LLFP, presented earlier by the authors. The second system, CLLFP?, features the possibility of invoking the oracle to obtain a witness satisfying a given constraint. We discuss encodings of Fitch-Prawitz Set theory, call-by-value lambda-calculi, and systems of Light Linear Logic. Finally, we show how to use Fitch-Prawitz Set Theory to define a type system that types precisely the strongly normalizing terms.

  13. Cognitive LF-Ant: A Novel Protocol for Healthcare Wireless Sensor Networks

    Directory of Open Access Journals (Sweden)

    Marcelo Alencar

    2012-08-01

    Full Text Available In this paper, the authors present the Cognitive LF-Ant protocol for emergency reporting in healthcare wireless sensor networks. The protocol is inspired by the natural behaviour of ants and a cognitive component provides the capabilities to dynamically allocate resources, in accordance with the emergency degree of each patient. The intra-cluster emergency reporting is inspired by the different capabilities of leg-manipulated ants. The inter-cluster reporting is aided by the cooperative modulation diversity with spectrum sensing, which can detect new emergency reporting requests and forward them. Simulations results show the decrease of average delay time as the probability of opportunistic access increases, which privileges the emergency reporting related to the patients with higher priority of resources’ usage. Furthermore, the packet loss rate is decreased by the use of cooperative modulation diversity with spectrum sensing.

  14. Cone structure and focusing of VLF and LF electromagnetic waves at high altitudes in the ionosphere

    Science.gov (United States)

    Alpert, Ya. L.; Green, J. L.

    1994-01-01

    The frequency and angle dependencies of the electric field radiated by an electric dipole E = E(sub 0) cos omega(t) are studied through numerical calculations of absolute value of E in the VLF and LF frequency bands where F is less than or equal 0.02 to 0.05 f(sub b) in a model ionosphere over an altitude region of 800-6000 km where the wave frequency and electron gyrofrequency varies between F approximately 4-500 kHz and f(sub b) is approximately equal (1.1 to 0.2) MHz respectively. It is found that the amplitudes of the electric field have large maxima in four regions: close to the direction of the Earth magnetic field line B(sub 0) (it is called the axis field E(sub 0), in the Storey E(sub St), reversed Storey E(sub RevSt), and resonance E(sub Res) cones. The maximal values of E(sub 0), E(sub Res), and E(sub RevSt) are the most pronounced close to the lower hybrid frequency, F approximately F(sub L). The flux of the electric field is concentrated in very narrow regions, with the apex angles of the cones Delta-B is approximately (0.1-1) deg. The enhancement and focusing of the electric field increases with altitude starting at Z greater than 800 km. At Z greater than or equal to 1000 up to 6000 km, the relative value of absolute value of E, in comparison with its value at Z = 800 km is about (10(exp 2) to 10(exp 4)) times larger. Thus the flux of VLF and LF electromagnetic waves generated at high altitudes in the Earth's ionosphere are trapped into very narrow conical beams similar to laser beams.

  15. Cone structure and focusing of VLF and LF electromagnetic waves at high altitudes in the ionosphere

    Science.gov (United States)

    Alpert, Ya. L.; Green, J. L.

    1994-01-01

    The frequency and angle dependencies of the electric field radiated by an electric dipole E = E(sub 0) cos omega(t) are studied through numerical calculations of absolute value of E in the VLF and LF frequency bands where F is less than or equal 0.02 to 0.05 f(sub b) in a model ionosphere over an altitude region of 800-6000 km where the wave frequency and electron gyrofrequency varies between F approximately 4-500 kHz and f(sub b) is approximately equal (1.1 to 0.2) MHz respectively. It is found that the amplitudes of the electric field have large maxima in four regions: close to the direction of the Earth magnetic field line B(sub 0) (it is called the axis field E(sub 0), in the Storey E(sub St), reversed Storey E(sub RevSt), and resonance E(sub Res) cones. The maximal values of E(sub 0), E(sub Res), and E(sub RevSt) are the most pronounced close to the lower hybrid frequency, F approximately F(sub L). The flux of the electric field is concentrated in very narrow regions, with the apex angles of the cones Delta-B is approximately (0.1-1) deg. The enhancement and focusing of the electric field increases with altitude starting at Z greater than 800 km. At Z greater than or equal to 1000 up to 6000 km, the relative value of absolute value of E, in comparison with its value at Z = 800 km is about (10(exp 2) to 10(exp 4)) times larger. Thus the flux of VLF and LF electromagnetic waves generated at high altitudes in the Earth's ionosphere are trapped into very narrow conical beams similar to laser beams.

  16. Structural characterization and molecular identification of arbuscular mycorrhiza morphotypes of Alzatea verticillata (Alzateaceae), a prominent tree in the tropical mountain rain forest of South Ecuador.

    Science.gov (United States)

    Beck, Adela; Haug, Ingeborg; Oberwinkler, Franz; Kottke, Ingrid

    2007-10-01

    The vast majority of the highly diverse trees in the tropical mountain rain forest of South Ecuador form arbuscular mycorrhizas, and previous molecular investigations revealed a high diversity of fungi. In this study, we present a first trial to link fungal DNA-sequences with defined morphotypes characterized on the basis of partly new mycelial features obtained from field material of one tree species, Alzatea verticillata. Fine roots were halved lengthwise to study the mycelium anatomy on one half and to obtain fungal nuclear rDNA coding for the small subunit rRNA of Glomeromycota from the other half. Light microscopy revealed conspicuously large amounts of mycelium attaching to the surface of the rootlets. The mycelium formed fine- or large-branched appressoria-like plates, vesicles of regular or irregular shape, and very fine, multibranched structures ensheathed by septate hyphae. These previously undescribed features of the supraradical mycelia combined with intraradical mycelium structures were used for distinguishing of four main morphogroups and subordinate 14 morphotypes. DNA sequences of Glomus group A, Acaulospora and Gigaspora, were obtained and linked to three morphogroups. Two sequence types within Glomus group A could be tentatively associated to subordinate morphotypes.

  17. Inductive effect of the leaf mixture extract of Aloe buettneri, Justicia insularis, Dicliptera verticillata and Hibiscus macranthus on in vitro production of estradiol.

    Science.gov (United States)

    Telefo, P B; Moundipa, P F; Tchouanguep, F M

    2004-04-01

    In the course of a preliminary clarification of the mechanisms of the leaf mixture extract of Aloe buettneri, Justicia insularis, Dicliptera verticillata and Hibiscus macranthus, locally used to regulate the menstrual cycle and to treat dysmenorrhea or cases of infertility in women, pieces of proestrus rat ovary were incubated in the presence of increasing concentration of the plant extract and/or human Chorionic Gonadotropin (hCG). The in vitro production of estradiol and progesterone by ovarian cells of proestrus rat was significantly increased in the presence of various concentration of hCG (P < 0.05). The different concentration of the plant extract increased the production of estradiol by twofold. In addition, the in vitro production of estradiol by ovarian cells increased by 13-fold when they were incubated with hCG (0.1 IU/ml) and a concentration of 130 microg/ml of the plant extract. These results clearly attest the direct effects of some chemical components of the leaf mixture of the plants on ovarian steroidogenesis.

  18. LF-interpretability and language development: a study of verbal and nominal features in Greek normally developing and SLI children.

    Science.gov (United States)

    Tsimpli, I M

    2001-06-01

    This paper includes (i) a comparison of the developmental pattern of certain morphosyntactic features in normally developing (ND) Greek children with similar data from a group of children with specific language impairment and (ii) a new analysis of the differences found. The analysis is based on a minimalist notion of LF interpretability. Depending on the feature-specification of lexical items (i.e., [+/- interpretable] at LF), a different route of development follows. In addition, phonological salience of both interpretable and noninterpretable features is argued to play an important role in an account of crosslinguistic differences in both normal and exceptional development.

  19. Characteristics of Amorphous Silicon Nitride Films Deposited by LF-PECVD from SiH4/N2

    Institute of Scientific and Technical Information of China (English)

    ZHONG Zhi-qin; ZHANG Yi; YU Zhi-wei; DAI Li-ping; ZHANG Guo-jun; WANG Yu-mei; WANG Gang; WANG Shu-ya

    2009-01-01

    Amorphous silicon nitride films were deposited by low-frequency plasma-enhanced chemical vapor deposition(LF-PECVD) using silane and nitrogen as precursors. Characteristics such as deposition rate,surface morphology,and chemical composition were measured by spectroscopic ellipsometry(SE),atomic force microscope(AFM) and x-ray photoelectron spectroscopy(XPS).It was shown that amorphous silicon nitride film could be prepared by LF-PECVD with good uniformity and even surface.The XPS result indicated that a small quantity of oxygen was involved in the sample,which was discussed in this paper.

  20. DNA barcoding discriminates the noxious invasive species, floating pennywort (Hydrocotyle ranunculoides L.f.), from non-invasive relatives

    NARCIS (Netherlands)

    Wiel, van de C.C.M.; Schoot, van der J.; Valkenburg, van J.L.C.H.; Duistermaat, H.; Smulders, M.J.M.

    2009-01-01

    Floating pennywort (Hydrocotyle ranunculoides L.f.), a member of the plant family Araliaceae originating from North America, is an example of an invasive aquatic species posing serious problems to the management of waterways outside of its original distribution area in Australia and Western Europe.

  1. Safety and tolerability of the antimicrobial peptide human lactoferrin 1-11 (hLF1-11

    Directory of Open Access Journals (Sweden)

    Blijlevens Nicole MA

    2009-09-01

    Full Text Available Abstract Background The treatment of patients with haematological malignancies by means of haematopoietic stem cell transplantation (HSCT is often accompanied by life threatening infections. With emerging antimicrobial resistance there is an increased need for new agents, with a beneficial safety profile. Therefore we evaluated the safety of the promising new antimicrobial peptide human lactoferrrin 1-11 (hLF1-11 in healthy volunteers and patients. Methods We undertook a sequential, randomised, double-blind, placebo-controlled study using ascending single (0.005, 0.05, 0.5, 5 mg and multiple intravenous doses (0.5, 5 mg in healthy volunteers, and open-label, single intravenous 5 mg doses in autologous HSCT recipients. Results Single and multiple doses of hLF1-11 were tolerable up to 5 mg intravenously in healthy volunteers, while 5 mg single dose was tolerable in patients. Elevations in transaminases possibly related to treatment were reversible and not serious. Conclusion The new antimicrobial hLF1-11 is well tolerated in healthy volunteers with repeated daily doses up to 5 mg. The side-effect profile is very favourable for an antimicrobial, the only undesirable effect being a possible elevation of transaminases, which may be related to hLF1-11 although the current data do not allow conclusive interpretation of treatment relationship. A lower dose is recommended for the forthcoming multiple dosing studies in HSCT patients. Trial registration ClinicalTrials.gov: nct00509938.

  2. The Proteome of Biologically Active Membrane Vesicles from Piscirickettsia salmonis LF-89 Type Strain Identifies Plasmid-Encoded Putative Toxins

    Directory of Open Access Journals (Sweden)

    Cristian Oliver

    2017-09-01

    Full Text Available Piscirickettsia salmonis is the predominant bacterial pathogen affecting the Chilean salmonid industry. This bacterium is the etiological agent of piscirickettsiosis, a significant fish disease. Membrane vesicles (MVs released by P. salmonis deliver several virulence factors to host cells. To improve on existing knowledge for the pathogenicity-associated functions of P. salmonis MVs, we studied the proteome of purified MVs from the P. salmonis LF-89 type strain using multidimensional protein identification technology. Initially, the cytotoxicity of different MV concentration purified from P. salmonis LF-89 was confirmed in an in vivo adult zebrafish infection model. The cumulative mortality of zebrafish injected with MVs showed a dose-dependent pattern. Analyses identified 452 proteins of different subcellular origins; most of them were associated with the cytoplasmic compartment and were mainly related to key functions for pathogen survival. Interestingly, previously unidentified putative virulence-related proteins were identified in P. salmonis MVs, such as outer membrane porin F and hemolysin. Additionally, five amino acid sequences corresponding to the Bordetella pertussis toxin subunit 1 and two amino acid sequences corresponding to the heat-labile enterotoxin alpha chain of Escherichia coli were located in the P. salmonis MV proteome. Curiously, these putative toxins were located in a plasmid region of P. salmonis LF-89. Based on the identified proteins, we propose that the protein composition of P. salmonis LF-89 MVs could reflect total protein characteristics of this P. salmonis type strain.

  3. Establishment of 9L/F344 rat intracerebral glioma model of brain tumor stem cells

    Directory of Open Access Journals (Sweden)

    Zong-yu XIAO

    2015-04-01

    Full Text Available Objective To establish the 9L/F344 rat intracerebral glioma model of brain tumor stem cells.  Methods Rat 9L gliosarcoma stem-like cells were cultured in serum-free suspension. The expression of CD133 and nestin were tested by immunohistochemistry. A total of 48 inbredline male F344 rats were randomly divided into 2 groups, and 9L tumor sphere cells and 9L monolayer cells were respectively implanted into the right caudate nucleus of F344 rats in 2 groups. Survival time was observed and determined using the method of Kaplan-Meier survival analysis. Fourteen days after implantation or when the rats were dying, their brains were perfused and sectioned for HE staining, and CD133 and nestin were detected by immunohistochemistry.  Results Rat 9L tumor spheres were formed with suspension culture in serum-free medium. The gliomas formed in both groups were invasive without obvious capsule. More new vessels, bleeding and necrosis could be detected in 9L tumor spheres group. The tumor cells in both groups were positive for CD133 and nestin. There was no significant difference in the expression of CD133 and nestin between 2 groups (P > 0.05, for all. According to the expression of nestin, the tumors formed by 9L tumor sphere cells were more invasive. The median survival time of the rats bearing 9L tumor sphere cells was 15 d (95%CI: 15.219-15.781, and the median survival time of the rats bearing 9L monolayer cells was 21 d (95%CI: 20.395-21.605. There was significant difference between 2 groups (χ2 = 12.800, P = 0.000.  Conclusions 9L/F344 rat intracerebral glioma model of brain tumor stem cells is successfully established, which provides a glioma model for the future research. DOI: 10.3969/j.issn.1672-6731.2015.04.012

  4. Effect of friction stir welding on microstructure and corrosion behavior of LF6 aluminum alloy

    Science.gov (United States)

    Ghauri, Faizan Ali; Farooq, A.; Ahmad, A.; Deen, K. M.

    2017-03-01

    The LF6 aluminum alloy plates were joined by friction stir welding method. The tool rotational (1180 rpm) and transverse speed (0.56 mm s‑1) were kept constant during welding of 4 mm thick plates. The microstructural features, hardness and tensile properties of the welded samples were determined to evaluate the structural integrity in comparison with the base metal. The electrochemical behavior of base metal (BM), thermo-mechanically affected zone (TMAZ) and weld nugget zone (WNZ) was also investigated by potentiodynamic polarization and electrochemical impedance spectroscopy in 3.5% NaCl solution. The microstructural study revealed significant grain refinement and agglomeration of β (Mg2Al3) intermetallic precipitates in the WNZ. The relatively higher hardness and a decrease in the ductility (3%) also assured the formation of precipitates β precipitates in the WNZ welded samples. The fracture surface of welded sample also revealed the existence of β precipitates within the elongated dimples which may be considered as the crack initiation sites. The relatively lower corrosion rate (23.68 mpy) and higher charge transfer resistance (403 Ω cm2) of BM compared to WNZ could be associated with the galvanic dissolution of Al-matrix through competitive charge transfer and relaxation (adsorption/desorption of intermediate species) processes specifically at the vicinity of the β precipitates.

  5. Evaluation of four bioestimulantes and three sustrates in the propagation of Vallea stipularis L.f.

    Directory of Open Access Journals (Sweden)

    Sonia Rosero

    2017-06-01

    Full Text Available The research consisted in evaluating four biostimulants and three substrates for the propagation of Vallea stipularis L.f. in greenhouse conditions. The biostimulants corresponded to: plant hormone rooting (B1, agrohormones 10-10-10 (B2, ESPOCH biostimulant (B3 and Gron Gibb (B4. The substrates consisted of: 50% black soil + 25% peat + 25% sand (S1; 50% black soil + 25% peat + 25% humus (S2 and 50% peat + 50% humus (S3. For the study, it was used a randomized complete design (RCD with factorial arrangement, it was determined the coefficient of variation and the Tukey test at 5% for the separation of means. The application of the biostimulants in the substrates was done at the time of planting. According to the results obtained, the best biostimulants corresponded to B1 (plant hormone rooting, applied in the seeds for 15 minutes, with which the highest emergency percentage was obtained (70.96%; with biostimulant B2 (agrohormones 10-10-10, applied for five minutes it was obtained 70.67% and with B3 (Gron Gibb 70.37%. The best substrate for emergence of plants was: 50% peat + 50% humus with 73.67% of emergence, where the highest plant height and root length were achieved, instead, the ESPOCH biostimulant (microoganisms reported a 67, 11% emergency.

  6. INITIAL GROWTH OF Tectona Grandis L.f., IN DIFFERENT SPACINGS IN PARACATU, MG, BRAZIL

    Directory of Open Access Journals (Sweden)

    Renato Luiz Grisi Macedo

    2005-03-01

    Full Text Available This work evaluated the initial growth of Tectona grandis L.f (Teca at different spacings in the northwesternregion of the Minas Gerais State – Brazil. A randomized block experimental design was used. The treatments consisted of fivedifferent planting spacings (3 x 2 m; 6 x 2 m; 6 x 4 m and 12 x 2,5 m with five repetitions each. The permanent experimentalconsisted of an approximate area of 300 m². The survival rate, plant height, diameter at breast height, volume/plant, volume/ha,basal area/plant and basal area/ha, were determined 24 and 36 months after planting, the current increment between the twoevaluation periods was determined for all the characteristics previously cited. The analysis of the results allows concluding thatteca presents potential for plantation in the region. Teca presented the higher growth rate at the 3 x 2 m spacing, whereno intra-specific competitive effects were observed. The main factors that limited initial growth were the long period ofrain insufficiency associated to a high soil compacting level.

  7. Modelling concentrations of decamethylcyclopentasiloxane in two UK rivers using LF2000-WQX

    Energy Technology Data Exchange (ETDEWEB)

    Price, Oliver R., E-mail: oliver.price@unilever.co [Safety and Environmental Assurance Centre (SEAC), Unilever, Colworth Science Park, Sharnbrook, Bedfordshire MK44 1LQ (United Kingdom); Williams, Richard J.; Zhang, Zhong [Centre for Ecology and Hydrology, Crowmarsh Gifford, Wallingford, Oxfordshire OX10 8BB (United Kingdom); Egmond, Roger van [Safety and Environmental Assurance Centre (SEAC), Unilever, Colworth Science Park, Sharnbrook, Bedfordshire MK44 1LQ (United Kingdom)

    2010-02-15

    Current regulatory environmental exposure assessments for decamethylcyclopentasiloxane (D{sub 5}), used in a range of personal care products, are based on a number of erroneous assumptions. Using an estimated D{sub 5} flux to waste water of 11.6 mg cap{sup -1} d{sup -1}, a 95.2% removal rate in Sewage Treatment Plants (STP) and a dilution factor of 10 results in modelled surface water concentrations that are up to an order of magnitude higher than concentrations observed downstream of STPs in two UK rivers. A GIS-based water quality model (LF2000-WQX) was used to predict concentrations of D{sub 5} in two UK rivers. Assuming the STP removal rate is reasonable, a waste water flux of 2.4 mg cap{sup -1} d{sup -1} is needed in order to obtain a reasonable match between predicted and observed in-river concentrations. This flux is consistent with measured effluent concentrations. The results highlight major uncertainties in estimating chemical emission rates for volatile chemicals used in personal care products and suggest that measured concentrations in waste water are needed to refine exposure assessments. - Surface water modelling of decamethylcyclopentasiloxane.

  8. Exploiting LF/MF signals of opportunity for lower ionospheric remote sensing

    Science.gov (United States)

    Higginson-Rollins, Marc A.; Cohen, Morris B.

    2017-08-01

    We introduce a method to diagnose and track the D region ionosphere (60-100 km). This region is important for long-distance terrestrial communication and is impacted by a variety of geophysical phenomena, but it is traditionally very difficult to detect. Modern remote sensing methods used to study the D region are predominately near the very low frequency (VLF, 3-30 kHz) band, with some work also done in the high-frequency and very high frequency bands (HF/VHF, 3-300 MHz). However, the frequency band between VLF and HF has been largely ignored as a diagnostic tool for the ionosphere. In this paper, we evaluate the use of 300 kHz radio reflections as a diagnostic tool for characterizing the D region of the ionosphere. We present radio receiver data, analyze diurnal trends in the signal from these transmitters, and identify ionospheric disturbances impacting LF/MF propagation. We find that 300 kHz remote sensing may allow a unique method for D region diagnostics compared to both the VLF and HF/VHF frequency bands, due to a more direct ionospheric reflection coefficient calculation method with high temporal resolution without the use of forward modeling.

  9. Management of Mauritia flexuosa L.f. for the handicraft production in Orinoco high plains, Colombia

    Directory of Open Access Journals (Sweden)

    Laura Isabel Mesa Castellanos

    2017-01-01

    Full Text Available We present information about the spear leaves harvest, fiber processing and trade of Mauritia flexuosa L.f. for the handicrafts production by the Sikuani people in Wacoyo indigenous Village, in Puerto Gaitán, Meta, Colombia. We made  a semi-structured interviews and workshops with harvesters, artisans and traders. During these sessions we inquired about their harvesting techniques, characteristics of harvestable individuals, distribution area of the palms, market aspects  and other uses of the species. Mauritia flexuosa, locally known as moriche, is one of the economic livelihoods for the artisans who inhabit the Wacoyo reservation. The harvest of spear leaves is carried out  in juvenile and subadult palms. The fibers are extracted from the spear leaves to manufacture handicrafts like bags, baskets, hats and hammocks. Artisans sell the products to tourists visiting the reservation, and intermediary sellers who commercialize them in the municipality center of Puerto Gaitán and Puerto Lopez, Meta. The use of M. flexuosa has the potential to become a sustainable activity, since the leaf harvest does not involve the death of the individuals.

  10. Wound healing activity of the leaves of Wattakaka volubilis (L.f. Stapf (Asclepiadaceae

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    V L Ashoka Babu

    2012-08-01

    Full Text Available Summary: The aqueous and alcohol extracts of the leaves of Wattakaka volubilis (L.f. Stapf were investigated for wound healing potential by excision, incision and dead space wound models in Wistar rats. Significant wound contraction and skin breaking strength, decrease in epithelization period, increased granulation tissue formation with high breaking strength were observed in animals treated with the extracts. Histopathological sections from the alcohol extract 400 µg/ml treated animals showed predominant collagen formation with scanty macrophages whereas samples from other extract treated groups showed moderate number of macrophages, collagen formation and fibroblast. HPTLC fingerprinting studies indicated the presence of flavonoids in alcohol and aqueous extracts of the leaves of W. volubilis. Industrial relevance: The leaves of Wattakaka volubilis is traditionally used in the treatment of various types of wounds. However there is no scientific data available to support this. Hence we conducted this study to evaluate the wound healing potential of this plant in three different wound models. The phytoconstituents responsible for the wound healing property could be isolated and developed into suitable formulations which would be an effective option as a wound healing drug, on a commercial scale. Keywords: Dead space wound; Excision wound; Incision wound; Wattakaka volubilis.

  11. 葵花籽油贮藏过程理化性质与LF-NMR弛豫特性的相关性%Relationship between Analytical Indicators of Sunflower Oil and the LF-NMR Relaxation Characteristics During Storage Process

    Institute of Scientific and Technical Information of China (English)

    卢海燕; 王欣; 赵婷婷; 刘宝林

    2014-01-01

    该文主要对葵花籽油分别在25、65℃避光贮藏过程的理化性质及其低场核磁共振(LF-NMR)弛豫特性的变化规律进行了研究,建立并验证了理化指标与LF-NMR检测结果的相关性模型.结果表明:65℃贮藏时,油样的过氧化值(PV)、共轭二烯值(K232)、共轭三烯值(K270)、p-茴香胺值(p-AV)、总极性化合物(TPC)含量及峰面积比例(S21、S22)随时间延长呈指数关系增加(r2 >0.93),而S23峰及单组分弛豫时间(T2W)则随时间延长呈指数减小趋势(r2 >0.98),通过多元回归分析可建立理化指标与弛豫特性间的相关性模型(r2> 0.94).25℃贮藏过程中理化性质及其LF-NMR弛豫特性的变化程度均相对较小,但PV、K232及TPC含量仍与贮藏时间呈良好的线性增大关系(r2 >0.95),其余理化指标及LF-NMR弛豫特性则无明显的规律性变化,相关性模型亦较差.

  12. Fatigue properties improvement of welded joints for LF21 aluminum alloy by ultrasonic peening method%超声冲击法改善LF21铝合金焊接接头的疲劳性能

    Institute of Scientific and Technical Information of China (English)

    王东坡; 霍立兴; 张玉凤; 陈俊梅; 刘允辉

    2001-01-01

    利用自行研制的超声冲击实验装置, 对提高LF21 铝合金焊接接头的疲劳强度进行了研究, 进行了TIG焊LF21铝合金非承载十字与T型接头的焊态及冲击处理态对比疲劳试验. 结果表明: 使用TIG焊获得的LF21铝合金原始焊接接头的疲劳强度与其它焊接方法得到的同样接头的疲劳强度基本相当,试图采用更高强度级别的S-N曲线对其进行设计时应持慎重态度. 在应力比R=0.5时, 超声冲击处理使LF21铝合金非承载十字焊接接头(拉伸载荷 )的疲劳强度提高了26%, 接头寿命延长了2~4倍. 在应力比R=0.1时, 超声冲击处理使T型接头(三点弯曲载荷)的疲劳强度提高了37%,接头寿命延长了4~8倍. 在应力比R=-1时, 超声冲击处理使T型接头(三点弯曲载荷)疲劳强度提高了44%, 接头寿命延长了5~10倍.

  13. Reports from the Bavarian State Agency for Environmental Protection (6). Technical reports. Information from LfU; Berichte aus dem Bayerischen Landesamt fuer Umweltschutz (6). Fachbeitraege. Informationen aus dem LfU

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1994-12-31

    In 1993 again, the Bavarian State Agency for Environmental protection had a broad range of tasks to fulfill. They form the subject of the 29 contributions. Described is not only the monitoring of radioactive and non-radioactive environmental pollutants but also the extension of the nuisance measuring grid. The cooperation with Russia in the southern Ural regarding the in-vivo determination of strontium 90 and caesium 137 in exposed persons is dealt with. The section `reports from LfU` covers also projects in the areas of nature conservation and care of the landscape. (DG) [Deutsch] Das bayerische LfU hatte auch im Jahr 1993 umfangreiche Aufgaben zu erfuellen. Die 29 Berichte geben hierueber einen Ueberblick, wobei neben der Erfahrung radioaktiver und nichtradioaktiver Umweltschadstoffe auch der Ausbau des Immissionsmessnetzes beschrieben wird. Auf die Zusammenarbeit mit Russland im Suedural in Bezug der In-vivo-Bestimmung von Strontium 90 und Caesium 137 an exponierten Personen wird eingegangen. Die Mitteilungen aus dem LfU umfassen auch Projekte auf dem Gebiet des Naturschutzes und der Landschaftspflege. (DG)

  14. Development and evaluation of teak (Tectona grandis L.f. taper equations in northern Thailand

    Directory of Open Access Journals (Sweden)

    Andrew J. Warner

    2016-09-01

    Full Text Available Taper refers to the general decrease in the regular outline of a solid body from its base to its tip. Taper models are used to estimate the volume and value of wood products from harvesting trees. Teak (Tectona grandis L.f. is highly valued as one of the world's most preferred timbers and a teak taper equation is required to inform optimal harvesting strategies given the limited plantation resource available in Thailand. Teak taper equations were developed and evaluated based on 331 sample trees collected in 2014 from eight plantations in northern Thailand aged from 10 to 46 yr using two taper model formulations—the Kozak variable-exponent taper model and the Goodwin cubic polynomial model comprising hyperbolic and parabolic terms. Variants based on both model types were fitted using nonlinear regression analysis with diameter at breast height, total tree height and height of girth measurement as the independent variables to estimate diameter underbark at the nominated height. Goodness-of-fit and leave-one-out cross validation with lack-of-fit statistical testing combined with extensive graphical analysis of residuals were used to select the best model. A Goodwin model variant (named FIO-teak1 as the first plantation teak taper model known to be published in Thailand provided the best estimates of volume and diameter underbark. A simple case study confirmed that FIO-teak1 in combination with the Farm Forestry Toolbox software package could assist teak plantation managers in decision making associated with optimizing log grade value based on standing tree inventory data.

  15. In vitro Free Radical Scavenging Potential of Indian Habitant Anisochilus carnosus (L.F.) Wall

    Institute of Scientific and Technical Information of China (English)

    Jaykumar Bhagat; Richard Lobo; Vinit Parmar; Mamatha Ballal

    2011-01-01

    AIM:To evaluate in vitro anti-oxidant potential of methanolic and aqueous extract of Indian habitants Anisochilus carnosus (L.F.) wall.,which is an annual shrub belonging to the family Lamiaceae commonly known as mint family.METHODS:The present study describes the effects by DPPH,alkaline DMSO,scavenging of ferric ion,NO,H2O2.ABTS models.Both extracts showed good free radical scavenging property which was calculated as IC50.RESULTS:IC50 of aqueous and methanolic extracts were found to be 7.09 and 5.77 μg.mL-1 for DPPH,8.14 and 13.29 μg·mL-1 for alkaline DMSO,27.16 and 31.25 μg·mL-1 for scavenging of ferric ion,13.82 and 26.94 μg.mL-1 for NO,13.82 and 26.94 μg.mL-1 for H2O2,113.84 and 128.03 μg.mL-1 for ABTS,whic1h were compared with Ascorbic acid (DPPH,scavenging of ferric ion,NO,ABTS) and rutin (alkaline DMSO,H2O2).Total antioxidant capacity of the extract was found to be 213 and 172 μg.mL-1 ascorbic acid for methanolic and aqueous extracts respectively.CONCLUSION:A.carnosus leaf extracts exhibited potent free radical scavenging activity.The overall antioxidant activity is attributed to its polyphenolic and other phytochemical constituents.The findings suggest that A.carnosus leaves could be a potential source of natural antioxidant in preventing or slowing the progression of aging and age-associated oxidative stress-related degenerative diseases.

  16. Nutrient concentration age dynamics of teak (Tectona grandis L.f.) plantations in Central America

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez-Moya, J.; Murillo, R.; Portuguez, E.; Fallas, J. L.; Rios, V.; Kottman, F.; Verjans, J. M.; Mata, R.; Alvarado, A.

    2013-05-01

    Aim of study. Appropriate knowledge regarding teak (Tectona grandis L.f.) nutrition is required for a better management of the plantations to attain high productivity and sustainability. This study aims to answer the following questions: How can it be determined if a teak tree suffers a nutrient deficiency before it shows symptoms? Are nutrient concentration decreases in older trees associated with age-related declines in forest productivity? Area of study. Costa Rica and Panama. Material and Methods. Nutrient concentration in different tree tissues (bole, bark, branches and foliage) were measured at different ages using false-time-series in 28 teak plantations Research highlights. Foliar N concentration decreases from 2.28 in year 1 to 1.76% in year 19. Foliar Mg concentration increases from 0.23 in year 1 to 0.34% in year 19. The foliar concentrations of the other nutrients are assumed to be constant with tree age: 1.33% Ca, 0.88% K, 0.16% P, 0.12% S, 130 mg kg{sup -}1 Fe, 43 mg kg{sup -}1 Mn, 11 mg kg{sup -}1 Cu, 32 mg kg{sup -}1 Zn and 20 mg kg{sup -}1 B. The nutrient concentration values showed can be taken as a reference to evaluate the nutritional status of similar teak plantations in the region. The concentrations of K, Mg and N could be associated with declines in teak plantation productivity as the plantation becomes older. Whether age-related changes in nutrient concentrations are a cause or a consequence of age-related declines in productivity is an issue for future research with the aim of achieving higher growth rates throughout the rotation period. (Author) 35 refs.

  17. Effect of low frequency (LF) electric fields on gene expression of a bone human cell line.

    Science.gov (United States)

    Caputo, Mariella; Zirpoli, Hylde; De Rosa, Maria Caterina; Rescigno, Tania; Chiadini, Francesco; Scaglione, Antonio; Stellato, Claudia; Giurato, Giorgio; Weisz, Alessandro; Tecce, Mario Felice; Bisceglia, Bruno

    2014-12-01

    We evaluated the effects, on cultured human SaOS-2 cells, of exposures to the low frequency (LF) electric signal (60 kHz sinusoidal wave, 24.5 V peak-to-peak voltage, amplitude modulated by a 12.5 Hz square wave, 50% duty cycle) from an apparatus of current clinical use in bone diseases requiring regenerating processes. Cells in flasks were exposed to a capacitively coupled electric field giving electric current density in the sample of 4 µA/cm(2). The whole expressed cellular mRNAs were systematically analyzed by "DNA microchips" technology to identify all individual species quantitatively affected by field exposure. Comparisons were made between RNA samples from exposed and control sham-exposed cells. Results indicated that immediately and 4 h after exposure there were almost no differentially modulated mRNA species. However, samples obtained at 24 h after exposure showed a small number of limitedly differential signals (7 down-regulated and 3 up-regulated with a cut-off value of ±1.5; 38 and 11, respectively, with a cut-off value of ±1.3), which included mostly mRNA encoding transcription factors and DNA binding proteins. Nevertheless, in identical experimental conditions, we previously demonstrated enzymatic changes of alkaline phosphatase occurring immediately after exposure and declining in a few hours. Therefore, since enzymatic changes occur before those observed at gene regulation level, it is conceivable that only earlier effects are directly due the treatment and then these effects are later able to affect gene expression only indirectly.

  18. Statistical study of seismo-ionospheric perturbations around Japan by using VLF/LF transmitters with a focal mechanism

    Science.gov (United States)

    Kawano, Tomoki; Hobara, Yasuhide; Tatsuta, Kenshin

    2016-04-01

    In this paper we perform the statistical analysis to study the response of the lower ionosphere prior to major seismic activities focusing on different earthquake types. The lower ionospheric condition is represented by daily averaged nighttime electric amplitude from various VLF/LF transmitter signals received in Japan by UEC team. Six-year record of ionospheric conditions are used for our data analysis. Over 200 earthquakes occurred around the VLF/LF transmitter - receiver paths during the time period of analysis. They are characterized into three different groups based on the Centroid-Moment-Tensor (CMT) solution such as reverse fault type, normal fault type and stress slip type. The ionospheric anomaly is identified by a large change (2 sigma criteria) in the VLF/LF daily nighttime amplitude. As a result, the highest occurrence rate of ionospheric anomaly is obtained for reverse type fault for both sea and ground earthquakes. The occurrence rate for these earthquakes are statistically significant because they are significantly large in comparison to those calculated from random test. The difference of occurrence rate of the ionospheric perturbations may indicate the coupling efficiency of seismic activity into the overlaying ionosphere originated from the pre-seismic condition of earth's crust. We also perform the trend-based earthquake prediction. Alarm threshold in nighttime VLF amplitude with -3.5 sigma is found to be most effective and significant for the earthquake prediction by using lower ionospheric perturbations.

  19. L-F001, a novel multifunctional ROCK inhibitor, suppresses neuroinflammation in vitro and in vivo: Involvement of NF-κB inhibition and Nrf2 pathway activation.

    Science.gov (United States)

    Chen, Jingkao; Yin, Wei; Tu, Yalin; Wang, Shengnan; Yang, Xiaohong; Chen, Qiuhe; Zhang, Xiao; Han, Yifan; Pi, Rongbiao

    2017-03-16

    Microglia and astrocytes are largely responsible for inflammatory injury in the brain of Alzheimer's disease (AD). Increasing evidence has indicated that Rho kinase (ROCK) plays an important role in the regulation of neuroinflammation. Previously, we synthesized a new chemical entity L-F001 and proved its potential inhibitory effects on ROCK and oxidative stress. Here, we investigated the anti-inflammatory effects and the molecular mechanisms of L-F001 in vitro and in vivo. L-F001 remarkably suppressed lipopolysaccharides (LPS)-elevated expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) as well as LPS-induced production of nitric oxide (NO), reactive oxygen species, interleukin-6 (IL-6) and tumor necreactive oxygen speciesis factor-α (TNF-α) in microglial BV-2 cells and in cultured astrocytes. Furthermore, L-F001 inhibited the degradation of IκB and nuclear translocation of nuclear factor kappa B (NF-κB) p65 subunit. Moreover, L-F001 induced the upregulation of heme-oxygenase-1 (HO-1) and glutamate cysteine ligase modifier subunit (GCLM) expression, two downstream effectors of nuclear factor (erythroid-derived 2)-like 2 (Nrf2). It was interesting that L-F001 also activated phosphatidylinositol 3-kinase (PI3K) pathway and induced M1 (CD16/32, M1 marker)/ M2 (CD206, M2 maker) transition in BV-2 cells which was significantly blocked by a PI3K inhibitor, wortmannin. Finally, L-F001 markedly attenuated the level of pro-inflammatory mediators in a murine model of systemic acute brain inflammation induced by LPS. Taken together, these results indicate that the novel multifunctional ROCK inhibitor L-F001 suppresses neuroinflammation in vitro and in vivo via NF-κB inhibition and Nrf2 activation, suggesting that L-F001 may be a promising drug candidate for treating neuroinflammation-associated CNS diseases, including AD.

  20. Final Environmental Assessment: For the M270 Multiple Launch Rocket System (MLRS) Expanded Training Use Areas at Avon Park Air Force Range, Florida

    Science.gov (United States)

    2005-11-10

    panther Puma concolor coryi E E Florida black bear Ursus americanus floridans N T Reptiles and Amphibians Eastern indigo snake Drymarchon corais...hyacinth Eugenia uniflora Surinam cherry Hydrilla verticillata Hydrilla Hymenachne amplexicaulis West Indian marsh grass Imperata cylindrica Cogon grass...garnoti) are some amphibian and reptile species that occur within the built up areas of APAFR (U.S. Navy, 2004). Sub-tropical bird species noted to

  1. IN VITRO AND IN VIVO ANTIOXIDANT ACTIVITY OF BURITI FRUIT (MAURITIA FLEXUOSA L.F.).

    Science.gov (United States)

    da Rocha Romero, Amanda Batista; de Carvalho e Martins, Maria do Carmo; Moreira Nunes, Paulo Humberto; Ravenna Trindade Ferreira, Nayara; da Silva Brito, Ana Karolinne; Marinho da Cunha, Polliana Farias; de Lima, Alessandro; de Assis, Regina Célia; Moreira Araújo, Eva

    2015-11-01

    Introducción: estudios previos han demostrado que la fruta Burití (Mauritia flexuosa L.f). posee una alta concentración de ácidos grasos monoinsaturados, carotenoides, polifenoles y ácido ascórbico. Este estudio evaluó la actividad antioxidante in vitro e in vivo del Burití. Métodos: fueron determinadas la composición química, el contenido de compuestos fenólicos y de carotenoides tanto de la pulpa del Burití como de las raciones de alimento. La actividad antioxidante in vitro fue analizada utilizando el ensayo del radical 2,2-difenil-1-picrilhidrazil (DPPH). Ratas Wistar (21 días de edad) fueron asignadas al azar (n = 10) en grupos controles y grupos experimentales (alimentación enriquecida con pulpa de Burití). Después de 60 días, la actividad antioxidante in vivo se evaluó mediante la actividad enzimática de la catalasa y grupos sulfhidrílo no proteico (NPSH) en el hígado, y se cuantificó el malondialdehído (MDA) en plasma y tejidos. Resultados: la pulpa del Burití presentó alto contenido de ácido graso oleico (73,3%), compuestos fenólicos (192 ± 0,3 mg/100 g) y carotenoides (23,9 ± 0,5 mg/100 g), así como una elevada actividad antioxidante in vitro. La dieta enriquecida tenía mayor contenido de fenoles y carotenoides, y una mayor actividad antioxidante en comparación con la alimentación estándar (p < 0,05). No se observaron diferencias entre los grupos con respecto a la actividad de la catalasa en el hígado y las concentraciones de MDA en plasma, hígado y riñones. Las ratas macho del grupo experimental tuvieron concentraciones hepáticas más altas de NPSH (p < 0,05). Conclusión: estos resultados pueden corroborar la hipótesis de que la fruta Burití es un alimento funcional antioxidante y su consumo es conveniente en una dieta nutricionalmente equilibrada.

  2. LF拓扑空间中的r-Lindel(o)ff可数性质%The r-Lindel(o)ff Countable Property in LF Topological Spaces

    Institute of Scientific and Technical Information of China (English)

    王延军

    2008-01-01

    在LF拓扑空间中借助LF-r闭集定义了r远域族与r-远域族,进一步引入r-Lindel(o)ff可数性和弱r-Lindel(o)ff可数性的概念,证明了r-Lindel可数性和弱r-Lindel可数性对于LF-r闭子集是遗传的,是r拓扑性质.

  3. Design of double CAN port syn-communication board based on TMS320LF2407A%基于TMS320LF2407A的双CAN口同步通讯板设计

    Institute of Scientific and Technical Information of China (English)

    王峰; 周希鹏; 荣溪超; 王锋

    2009-01-01

    为将高可靠性、模块化的车栽PLC控制器应用到防空武器火力控制分系统,提高其战场条件下的可靠性和可维修性,针对某型防空武器内部TTCAN网络的时间触发特性.提出一种基于TMS320LF2407和CAN控制器MCP2515的双CAN口同步通讯板的新方案,解决了PLC与TTCAN网络的接口问题.

  4. Functional receptor molecules CD300lf and CD300ld within the CD300 family enable murine noroviruses to infect cells

    Science.gov (United States)

    Haga, Kei; Fujimoto, Akira; Takai-Todaka, Reiko; Miki, Motohiro; Doan, Yen Hai; Murakami, Kosuke; Yokoyama, Masaru; Murata, Kazuyoshi; Nakanishi, Akira; Katayama, Kazuhiko

    2016-01-01

    Norovirus is the leading cause of acute gastroenteritis worldwide. Since the discovery of human norovirus (HuNoV), an efficient and reproducible norovirus replication system has not been established in cultured cells. Although limited amounts of virus particles can be produced when the HuNoV genome is directly transfected into cells, the HuNoV cycle of infection has not been successfully reproduced in any currently available cell-culture system. Those results imply that the identification of a functional cell-surface receptor for norovirus might be the key to establishing a norovirus culture system. Using a genome-wide CRISPR/Cas9 guide RNA library, we identified murine CD300lf and CD300ld as functional receptors for murine norovirus (MNV). The treatment of susceptible cells with polyclonal antibody against CD300lf significantly reduced the production of viral progeny. Additionally, ectopic CD300lf expression in nonsusceptible cell lines derived from other animal species enabled MNV infection and progeny production, suggesting that CD300lf has potential for dictating MNV host tropism. Furthermore, CD300ld, which has an amino acid sequence in the N-terminal region of its extracellular domain that is highly homologous to that of CD300lf, also functions as a receptor for MNV. Our results indicate that direct interaction of MNV with two cell-surface molecules, CD300lf and CD300ld, dictates permissive noroviral infection. PMID:27681626

  5. The effect of dielectric top lids on materials processing in a low frequency inductively coupled plasma (LF-ICP) reactor

    Science.gov (United States)

    Lim, J. W. M.; Chan, C. S.; Xu, L.; Xu, S.

    2014-08-01

    The advent of the plasma revolution began in the 1970's with the exploitation of plasma sources for anisotropic etching and processing of materials. In recent years, plasma processing has gained popularity, with research institutions adopting projects in the field and industries implementing dry processing in their production lines. The advantages of utilizing plasma sources would be uniform processing over a large exposed surface area, and the reduction of toxic emissions. This leads to reduced costs borne by manufacturers which could be passed down as consumer savings, and a reduction in negative environmental impacts. Yet, one constraint that plagues the industry would be the control of contaminants in a plasma reactor which becomes evident when reactions are conducted in a clean vacuum environment. In this work, amorphous silicon (a-Si) thin films were grown on glass substrates in a low frequency inductively coupled plasma (LF-ICP) reactor with a top lid made of quartz. Even though the chamber was kept at high vacuum ( 10-4 Pa), it was evident through secondary ion mass spectroscopy (SIMS) and Fourier-transform infra-red spectroscopy (FTIR) that oxygen contaminants were present. With the aid of optical emission spectroscopy (OES) the contaminant species were identified. The design of the LF-ICP reactor was then modified to incorporate an Alumina (Al2O3) lid. Results indicate that there were reduced amounts of contaminants present in the reactor, and that an added benefit of increased power transfer to the plasma, improving deposition rate of thin films was realized. The results of this study is conclusive in showing that Al2O3 is a good alternative as a top-lid of an LF-ICP reactor, and offers industries a solution in improving quality and rate of growth of thin films.

  6. LC2 and OsVIL2 Promote Rice Flowering by Photoperoid-Induced Epigenetic Silencing of OsLF

    Institute of Scientific and Technical Information of China (English)

    Jun Wang; Jiang Hu; Qian Qian; Hong-Wei Xue

    2013-01-01

    Proper flowering time is essential for plant reproduction.Winter annual Arabidopsis thaliana needs vernalization before flowering,during which AtVlLs (VlN3 and VRN5,components of PRC2 complex) mediate the H3K27 trimethylation at the FLC locus (a floral repressor) to repress the FLC expression and hence to induce flowering.However,how VlLs (VlL,VERNALIZATION INSENSITIVE 3-LIKE) function in rice is unknown.Here we demonstrated that rice LC2 (OsVlL3) and OsVll.2 (two OsVlLs,possible components of PRC2 complex) promote rice flowering.Our results showed that expressions of LC2 and OsVlL2 are induced by SD (short-day) conditions and both Ic2 mutant and OsVlL2-RNAi lines display delayed heading date,consistent with the reduced expression levels of Hdl and Hd3a.Interestingly,LC2 binds to the promoter region of a floral repressor OsLF and represses the OsLF expression via H3K27 tri-methylation modification.In addition,OsLF directly regulates the Hdl expression through binding to Hdl promoter.These results first demonstrated that the putative PRC2 in rice is involved in photoperiod flowering regulation,which is different from that of Arabidopsis,and revealed that LC2 binds the promoter region of target gene,presenting a possible mechanism of the recruitment process of PRC2 complex to its target genes.The studies provide informative clues on the epigenetic control of rice flowering.

  7. Characterization and applications of VLF/LF source locations from lightning using the Huntsville Alabama Marx Meter Array

    Science.gov (United States)

    Bitzer, Phillip M.; Christian, Hugh J.; Stewart, Mike; Burchfield, Jeff; Podgorny, Scott; Corredor, David; Hall, John; Kuznetsov, Evgeny; Franklin, Veronica

    2013-04-01

    Arrays that detect and locate the four-dimensional spacetime positions of radiation sources from lightning have largely utilized sensors sensitive to the very high frequency (VHF) regime with ˜ 15 km baselines or very low frequency/low frequency (VLF/LF) regime with ˜ 100 km baselines. This paper details initial results from the newly developed Huntsville Alabama Marx Meter Array (HAMMA), consisting of Marx meters (electric field change meters) sensitive to a frequency band ˜ 1 Hz to 400 kHz. The arrival time of HAMMA waveforms due to radiation sources from lightning are used to determine the spacetime position of these sources. The locations are compared with two well-documented and operational arrays, the National Lightning Detection Network (NLDN) and the North Alabama Lightning Mapping Array (NALMA). The standard deviation of the difference between HAMMA and NLDN locations of return strokes is 305 and 266 m in x and y, respectively, while the standard deviation of the difference between HAMMA and NALMA sources is 237, 226, and 688 m in x, y and z, respectively. We further show that NLDN intracloud locations differ in horizontal distance from the corresponding HAMMA locations by a median value of 479 m. In addition, we use HAMMA source locations to map several lightning flashes in the VLF/LF and show HAMMA sources largely map out the same electrical extent as VHF sources and provide unique insights to the properties of the discharges occurring. Finally, we show that VLF/LF sources can determine the leader polarity in several example flashes but not necessarily whether a flash comes to ground. Copyright 2013 American Geophysical Union. All rights reserved.

  8. Software design of serial communication between TMS320LF2407A and PC based on modbus%基于MODBUS协议的TMS320LF2407A与PC机串口通信软件设计

    Institute of Scientific and Technical Information of China (English)

    王欣; 文小玲; 刘义亭; 孙谋

    2011-01-01

    This paper introduces the software design method of slave serial communications between the master PC and slave TMS320LF2407A DSP based on Modbus.The interrupt mode is used for the data receiving and between the master and the slave in order to guarantee the reliability of the communication.The DSP communication software is programmed using C language and can be easily transplanted to other microprocessors.The experimental results show that phase A input current of nonlinear load diode rectifier circuit can be displayed by the Labview, realizing the simple, reliable and stable data transmission between DSP and PC.%主要阐述了基于MODBUS协议的DSP从机通信软件的设计方法.以PC机作为上位机,TMS320LF2407A DSP作为下位机,采用中断方式实现数据的接收和发送,保证了数据传输的可靠性.利用C语言编写DSP从站通信程序,以便于程序的移植.经实验验证,非线性负载二极管整流电路的A相输入电流波形能通过Labview显示,实现了DSP与PC机之间简单、可靠、稳定的数据传输.

  9. Biological Studies of Bagous Hydrillae

    Science.gov (United States)

    1994-09-01

    garis L. cultivated beans Raphanus sativus L. cultivated radish Sedum sp. introduced, ornamental Sesuvium portulacastrum (L.)L. native Appencix A...watermelon Matsum. & Nakai var. lanatus Citrus sp. cultivated citrus Crassula argentea Thunb. introduced, ornamental Cucumis sativus L. cultivated cucumber

  10. Leptospermum flavescens Constituent-LF1 Causes Cell Death through the Induction of Cell Cycle Arrest and Apoptosis in Human Lung Carcinoma Cells.

    Directory of Open Access Journals (Sweden)

    Suerialoasan Navanesan

    Full Text Available Leptospermum flavescens Sm. (Myrtaceae, locally known as 'Senna makki' is a smallish tree that is widespread and recorded to naturally occur in the montane regions above 900 m a.s.l from Burma to Australia. Although the species is recorded to be used traditionally to treat various ailments, there is limited data on biological and chemical investigations of L. flavescens. The aim of the present study was to investigate and understand the ability of L. flavescens in inducing cell death in lung cancer cells. The cytotoxic potentials of the extraction yields (methanol, hexane, ethyl acetate and water extracts as wells as a semi pure fraction, LF1 were evaluated against two human non-small cell lung carcinoma cell lines (A549 and NCI-H1299 using the MTT assay. LF1 showed the greatest cytotoxic effect against both cell lines with IC50 values of 7.12 ± 0.07 and 9.62 ± 0.50 μg/ml respectively. LF1 treated cells showed a sub-G1 region in the cell cycle analysis and also caused the presence of apoptotic morphologies in cells stained with acridine orange and ethidium bromide. Treatment with LF1 manifested an apoptotic population in cells that were evaluated using the Annexin V/ propidium iodide assay. Increasing dosage of LF1 caused a rise in the presence of activated caspase-3 enzymes in treated cells. Blockage of cell cycle progression was also observed in LF1-treated cells. These findings suggest that LF1 induces apoptosis and cell cycle arrest in treated lung cancer cells. Further studies are being conducted to isolate and identify the active compound as well to better understand the mechanism involved in inducing cell death.

  11. 污水处理中温度对黑藻生长的影响(英文)

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    [Objective] The research aimed to study the influence of temperature on the growth of Hydrilla verticillata in the wastewater treatment process.[Method] By using the single factor experiment design,four temperatures which were 5,15,25 and 35 ℃ were set.The growth situation of H.verticillata under the different temperatures was observed in the treatment process of domestic sewerage.[Result] H.verticillata grew luxuriantly at 35 and 25 ℃.Via the cultivation of 15 days,the plant length,branch number,root numbe...

  12. Inclusions in GCr15 bearing steel produced by 120 t LD-LF-VD-CC process%120t转炉-LF-VD-CC流程生产GCr15轴承钢的夹杂物

    Institute of Scientific and Technical Information of China (English)

    范植金; 罗国华; 冯文圣; 朱玉秀

    2011-01-01

    Inclusions in GCr15 bearing steel produced by 120 t LD-LF-VD-CC process were investigated using metallogaphic microscope analysis,electron probe microanalysis and inclusion electroanalysis.The results indicate that the A inclusion grade is 0.5-1.5,B inclusion grade is 0.5-1.5,C inclusion grade is 0,D inclusion grade is not more than 0.5.The results of inclusion grading satisfy requirements of the GB/T 18254—2002 standard.Average total amount of oxide inclusions in GCr15 bearing steel produced by LD-LF-VD-CC process is 0.0037% in mass percent,which is reduced in evidence compared with Al-killed GCr15 steel produced by electric furnace.The inclusions mainly are strip manganese sulfide,chain alumina,fusiform manganese sulfide enwrapping granular alumina,granular calcium aluminate,granular calcium sulfide enwrapping calcium aluminate,granular magnesium aluminum spinel,granular calcium sulfide enwrapping magnesium aluminum spinel,quadrate titanium(carbide)nitride,etc.%对120 t转炉-LF-VD-CC流程生产的GCr15轴承钢夹杂物进行了金相显微镜观察评级、电子探针观察分析和电解夹杂分析。结果显示:A类夹杂0.5~1.5级,B类夹杂0.5~1.5级,C类夹杂0级,D类夹杂不大于0.5级,可充分满足GB/T 18254—2002标准的规定;其氧化夹杂物总质量分数平均值为0.00370%,相比采用Al脱氧电炉冶炼的GCr15轴承钢明显降低;夹杂物类型主要有条状硫化锰、链状氧化铝、纺锤状硫化锰包覆粒状氧化铝、粒状铝酸钙、粒状硫化钙包覆铝酸钙、粒状镁铝尖晶石、粒状硫化钙包覆镁铝尖晶石、方块状氮(碳)化钛等。

  13. Cleanliness study of SWRH82 B hard wire steel produced by BOF--LF--CC processes%BOF--LF--CC生产SWRH82 B硬线钢的洁净度研究

    Institute of Scientific and Technical Information of China (English)

    张游游; 刘建华; 苏晓峰; 崔衡; 张杰; 季益龙

    2016-01-01

    The cleanliness evolution of SWRH82B steel produced by the BOF-LF-CC process was studied by systematic sampling and lab comprehensive analysis. The results show that the content of oxygen and the number of micro-inclusions significantly decrease after calcium treatment, while the content of nitrogen in molten steel slightly increases. Both the contents of oxygen and nitrogen sharp-ly increase during the casting process. 97% of the micro-inclusions in casting billets are smaller than 5μm. They mainly include trenchant angularity single Al2 O3 inclusions, spherical complex inclusions of oxides with CaS and MnS, and complex inclusions of sim-ple oxides or nitrides with MnS. Calcium treatment after LF refining removes oxygen from molten steel efficiently, but increase the amount of Al2 O3 inclusions in casting billets, which is harmful to the quality of SWRH82B steel. Due to serious lag entrapment, re-fractory erosion and reoxidation caused by unreasonable tundish structure, the cleanliness of liquid steel decreases.%采用系统取样与实验室综合分析,对BOF-LF-CC工艺生产的SWRH82B钢洁净度衍变规律进行研究。结果表明,钙处理后钢中T[ O]和显微夹杂物数量显著下降,轻微增氮;浇铸过程增氧增氮严重;铸坯中<5μm显微夹杂占97%,主要是棱角分明的纯Al2 O3夹杂,球状复合氧化物与CaS和MnS的复合夹杂,简单氧化物或氮化物与MnS的复合夹杂;LF精炼后钙处理,脱氧显著,但使得铸坯中出现很多Al2 O3硬性夹杂,对SWRH82B盘条质量造成严重的危害;中间包结构不合理,卷渣、内衬侵蚀和二次氧化严重,连铸过程钢液洁净度下降。

  14. Occurrence of the Microcystins MC-LW and MC-LF in Dutch Surface Waters and Their Contribution to Total Microcystin Toxicity

    Directory of Open Access Journals (Sweden)

    Elisabeth J. Faassen

    2013-07-01

    Full Text Available Microcystins (MCs are the most frequently found cyanobacterial toxins in freshwater systems. Many MC variants have been identified and variants differ in their toxicity. Recent studies showed that the variants MC-LW and MC-LF might be more toxic than MC-LR, the variant that is most abundant and mostly used for risk assessments. As little is known about the presence of these two variants in The Netherlands, we determined their occurrence by analyzing 88 water samples and 10 scum samples for eight MC variants ((dm-7-MC-RR, MC-YR, (dm-7-MC-LR, MC-LY, MC-LW and MC-LF by liquid chromatography with tandem mass spectrometry detection. All analyzed MC variants were detected, and MC-LW and/or MC-LF were present in 32% of the MC containing water samples. When MC-LW and MC-LF were present, they contributed to nearly 10% of the total MC concentrations, but due to their suspected high toxicity, their average contribution to the total MC toxicity was estimated to be at least 45%. Given the frequent occurrence and possible high toxicity of MC-LW and MC-LF, it seems better to base health risk assessments on the toxicity contributions of different MC variants than on MC-LR concentrations alone.

  15. Development of X60 Pipeline Steel by 150 t EAF-LF-CSP Process at Zhujiang Steel%珠钢150 t EAF-LF-CSP工艺X60管线钢的开发

    Institute of Scientific and Technical Information of China (English)

    毛新平; 庄汉洲; 李春艳; 刘清友; 李烈军; 陈学文; 高吉祥; 陈麒琳

    2006-01-01

    珠钢用150 t EAF-LF-50~60 mm板坯连铸机-热连轧工艺和Nb-Ti复合微合金化技术开发了9.50 mm带X60管线钢(%:0.03~0.07C,1.2~1.6Mn,Nb+V+Ti≤0.15).检验结果表明,钢带组织为铁素体+少量珠光体,晶粒度11.5~12.0级,带状组织≤1.5级,屈服强度440~505 MPa,0 ℃夏比冲击功为140~270 J,其综合性能满足API-5L技术标准和工程使用要求.

  16. Development of Tencel-LF Raised Floral Fabric%Tencel—LF起花织物的开发

    Institute of Scientific and Technical Information of China (English)

    万惠群

    2012-01-01

    介绍了Tencel—LF起花织物的风格、外观及组织设计特点,详述了其织造生产中的浆料配方、浆纱工艺及织造工艺等关键技术条件;该织物织造生产效率达到93%以上,入库一等品率96%以上,取得了良好的经济效益。%The style of Tencel-LF raised floral fabric, appearance and characteristics of organizational design were introduced. The key technology of size formula, sizing and weaving process in weaving production were detailed. The production practice indicated that the weaving efficiency reached above 93%, the first grade percentage put in storage reached above 96% and achieved good economic benefit.

  17. A Review of Nonlinear Low Frequency (LF) Wave Observations in Space Plasmas: On the Development of Plasma Turbulence

    Science.gov (United States)

    Tsurutani, Bruce T.

    1995-01-01

    As the lead-off presentation for the topic of nonlinear waves and their evolution, we will illustrate some prominent examples of waves in space plasmas. We will describe recent observations detected within planetary foreshocks, near comets and in interplanetary space. It is believed that the nonlinear LF plasma wave features discussed here are part of and may be basic to the development of plasma turbulence. In this sense, this is one area of space plasma physics that is fundamental, with applications to fusion physics and astrophysics as well. It is hoped that the reader(s) will be stimulated to study nonlinear wave development themselves, if he/she is not already involved.

  18. Helicusin E, Isochromophilone X and Isochromophilone XI: New Chloroazaphilones Produced by the Fungus Bartalinia robillardoides Strain LF550

    Directory of Open Access Journals (Sweden)

    Johannes F. Imhoff

    2013-03-01

    Full Text Available Microbial studies of the Mediterranean sponge Tethya aurantium led to the isolation of the fungus Bartalinia robillardoides strain LF550. The strain produced a number of secondary metabolites belonging to the chloroazaphilones. This is the first report on the isolation of chloroazaphilones of a fungal strain belonging to the genus Bartalinia. Besides some known compounds (helicusin A (1 and deacetylsclerotiorin (2, three new chloroazaphilones (helicusin E (3; isochromophilone X (4 and isochromophilone XI (5 and one new pentaketide (bartanolide (6 were isolated. The structure elucidations were based on spectroscopic analyses. All isolated compounds revealed different biological activity spectra against a test panel of four bacteria: three fungi; two tumor cell lines and two enzymes.

  19. Elodea canadensis under N and CO2 limitation : Adaptive changes in Rubisco and PEPCase activity in a bicarbonate user

    NARCIS (Netherlands)

    Van Ginkel, LC; Schutz, [No Value; Prins, HBA

    2000-01-01

    Diffusion of CO2 in water is 10,000 times slower than in air. Because of this photosynthesis in submerged aquatic macrophytes is often limited by CO2 availability. Elodea canadensis shows HCO3- utilization under conditions of CO2 limitation. A closely related species, Hydrilla verticillata, which al

  20. Ionospheric perturbations related to the earthquake in Vrancea area on November 22, 2014, as detected by electromagnetic VLF/LF frequency signals

    Directory of Open Access Journals (Sweden)

    Maria Solovieva

    2015-11-01

    Full Text Available Data from the European network of very low/ low frequency (VLF/LF receivers has been used to study the response of the lower ionosphere to the earthquake of magnitude 5.5 in Vrancea area on November 22, 2014. Negative amplitude anomalies have been observed during 3 days before the earthquake and two days after, on the LF (45.9 kHz signal passed above the seismic area. No perturbations have been found for the same signal in control paths during this period. Other possible influences both from above and below which can produce perturbations in the ionosphere have been taken into consideration.

  1. Frequency Measurement of FDR Based on Soil Dielectric Spectrum in LF-UHF%基于土壤LF-UHF频段介电特性的FDR测量频率研究

    Institute of Scientific and Technical Information of China (English)

    许景辉; 马孝义; Sally D Logsdon; Robert Horton

    2013-01-01

    土壤介电特性是影响FDR传感器测量土壤含水量精度和适应性的重要物理参数.为了确定28 mm长、10 mm间距的7探针FDR传感器理想频率,通过矢量网络分析仪对4种不同土壤的LF-UHF频段(1 MHz ~3 GHz)散射参数进行了测量,通过Logsdon和Laird模型变换,使用Matlab进行了4种土壤表观介电常数的计算和绘制.通过土壤介电频谱分析,研究了土壤极化机理,分析了土壤介电特性与频率的关系,确定了FDR传感器的工作频段为44 ~ 398 MHz,考虑到温度对极化的影响,其最佳工作频段为62 ~ 110 MHz,其中75 MHz是其工作的最佳频率.此频率下土壤介电值仅对土壤含水量敏感,是消除温度等因素对FDR传感器影响的理想频率.%Soil dielectric property is an important physical parameter affecting the measuring accuracy and adaptation of moisture content by using FDR sensor.In order to obtain the best frequency of 7-probes FDR with length of 28 mm and needle-to-needle spacing of 10 mm,scattering parameters of LF-UHF wave bands (1 MHz ~ 3 GHz) in four kinds of soil were measured by using VNA.Though transformation based on Logsdon model and Laird model,four soil apparent dielectric permittivities were calculated and figured in Matlab.The mechanism of soil polarization was analyzed by soil dielectric spectrun analysis.The relationship between soil dielectric property and frequency was discussed.The working frequency of 44 ~ 398 MHz for FDR sensor was confirmed.Taking temperature into consideration,the ideal working frequency band was 62 ~ 110 MHz in which 75 MHz was the best.The soil dielectric value was only sensitive to soil moisture content under the best frequency.It was the ideal frequency for eliminating the effect of temperature and other factors on FDR sensor.

  2. Phospho-MEK1/2 and uPAR Expression Determine Sensitivity of AML Blasts to a Urokinase-Activated Anthrax Lethal Toxin (PrAgU2/LF

    Directory of Open Access Journals (Sweden)

    Amira Bekdash

    2015-10-01

    Full Text Available In this study, we attempt to target both the urokinase plasminogen activator and the mitogen-activated protein kinase pathway in acute myeloid leukemia (AML cell lines and primary AML blasts using PrAgU2/LF, a urokinase-activated anthrax lethal toxin. PrAgU2/LF was cytotoxic to five out of nine AML cell lines. Cytotoxicity of PrAgU2/LF appeared to be nonapoptotic and was associated with MAPK activation and urokinase activity because all the PrAgU2/LF-sensitive cell lines showed both uPAR expression and high levels of MEK1/2 phosphorylation. Inhibition of uPAR or desensitization of cells to MEK1/2 inhibition blocked toxicity of PrAgU2/LF, indicating requirement for both uPAR expression and MAPK activation for activity. PrAgU2/LF was also cytotoxic to primary blasts from AML patients, with blasts from four out of five patients showing a cytotoxic response to PrAgU2/LF. Cytotoxicity of primary AML blasts was also dependent on uPAR expression and phos-MEK1/2 levels. CD34+ bone marrow blasts and peripheral blood mononuclear cells lacked uPAR expression and were resistant to PrAgU2/LF, demonstrating the lack of toxicity to normal hematological cells and, therefore, the tumor selectivity of this approach. Dose escalation in mice revealed that the maximal tolerated dose of PrAgU2/LF is at least 5.7-fold higher than that of the wild-type anthrax lethal toxin, PrAg/LF, further demonstrating the increased safety of this molecule. We have shown, in this study, that PrAgU2/LF is a novel, dual-specific molecule for the selective targeting of AML.

  3. Analysis on the Nitrogen Control of Pipeline Steel X70 in LF Process%X70管线钢LF控氮工艺分析

    Institute of Scientific and Technical Information of China (English)

    胡国旭

    2012-01-01

    The production process for X70 pipeline steel and the control nitrogen factors in LF process were comprehensively analyzed. The results show that the nitrogen pick-up in liquid steel is mainly attributed to the liquid steel exposed to atmosphere at the desulfurization stage under the white slag conditions in LF processs. Accordingly, six key technologies of nitrogen control for pipeline steel X70 from BOF tap to LF end-point were proposed. According to LF practice, constant perfecting and optimizing its technical parameters can lay a theoretical and technological foundation for producing high grade steel containing lower nitrogen.%对X70管线钢生产工艺及LF过程控氮因素进行了综合分析.结果表明,LF工况条件下造白渣脱硫阶段的“钢液裸露”是造成钢液增氮最为主要的原因.在此基础上提出了从BOF出钢到LF处理结束等6项X70管线钢LF控氮工艺要点.根据LF生产实践不断完善与优化其各环节的技术参数,为生产更低含氮量要求的高级别钢种奠定理论与工艺基础.

  4. 75 FR 77880 - Proposed Collection; Comment Request; GuLF Worker Study: Gulf Long-Term Follow-Up Study for Oil...

    Science.gov (United States)

    2010-12-14

    ... excessive heat, and possibly to stress due to widespread economic and lifestyle disruption. Exposures range... HUMAN SERVICES National Institutes of Health Proposed Collection; Comment Request; GuLF Worker Study: Gulf Long-Term Follow-Up Study for Oil Spill Clean-Up Workers and Volunteers SUMMARY: Under...

  5. Nonlinear Conte-Zbilut-Federici (CZF Method of Computing LF/HF Ratio: A More Reliable Index of Changes in Heart Rate Variability

    Directory of Open Access Journals (Sweden)

    Vernon Bond Jr

    2016-09-01

    Full Text Available Objectives: Acupuncture treatments are safe and effective for a wide variety of diseases involving autonomic dysregulation. Heart rate variability (HRV is a noninvasive method for assessing sympathovagal balance. The low frequency/high frequency (LF/HF spectral power ratio is an index of sympathovagal influence on heart rate and of cardi

  6. Ho:YLF & Ho:LuLF slab amplifier system delivering 200 mJ, 2 µm single-frequency pulses

    CSIR Research Space (South Africa)

    Strauss, HJ

    2011-07-01

    Full Text Available A single-frequency single-pass amplifier based on Ho:YLF and Ho:LuLF in a scalable slab architecture delivering up to 210 mJ at 2064 nm is demonstrated. The amplifier was end-pumped by a 1890 nm Tm:YLF slab laser and was seeded with a 69 mJ single...

  7. 75 FR 62132 - Proposed Collection; Comment Request; GuLF Worker Study: Gulf Long-Term Follow-Up Study for Oil...

    Science.gov (United States)

    2010-10-07

    ... HUMAN SERVICES National Institutes of Health Proposed Collection; Comment Request; GuLF Worker Study... proposed projects to be submitted to the Office of Management and Budget (OMB) for review and approval... to create a resource for additional collaborative research on focused hypotheses or subgroups. Over...

  8. LF-15 & T7, synthetic peptides derived from tumstatin, attenuate aspects of airway remodelling in a murine model of chronic OVA-induced allergic airway disease.

    Directory of Open Access Journals (Sweden)

    Karryn T Grafton

    Full Text Available BACKGROUND: Tumstatin is a segment of the collagen-IV protein that is markedly reduced in the airways of asthmatics. Tumstatin can play an important role in the development of airway remodelling associated with asthma due to its anti-angiogenic properties. This study assessed the anti-angiogenic properties of smaller peptides derived from tumstatin, which contain the interface tumstatin uses to interact with the αVβ3 integrin. METHODS: Primary human lung endothelial cells were exposed to the LF-15, T3 and T7 tumstatin-derived peptides and assessed for cell viability and tube formation in vitro. The impact of the anti-angiogenic properties on airways hyperresponsiveness (AHR was then examined using a murine model of chronic OVA-induced allergic airways disease. RESULTS: The LF-15 and T7 peptides significantly reduced endothelial cell viability and attenuated tube formation in vitro. Mice exposed to OVA+ LF-15 or OVA+T7 also had reduced total lung vascularity and AHR was attenuated compared to mice exposed to OVA alone. T3 peptides reduced cell viability but had no effect on any other parameters. CONCLUSION: The LF-15 and T7 peptides may be appropriate candidates for use as novel pharmacotherapies due to their small size and anti-angiogenic properties observed in vitro and in vivo.

  9. The Adherent/Invasive Escherichia coli Strain LF82 Invades and Persists in Human Prostate Cell Line RWPE-1, Activating a Strong Inflammatory Response

    Science.gov (United States)

    Aleandri, Marta; Marazzato, Massimiliano; Conte, Antonietta L.; Ambrosi, Cecilia; Nicoletti, Mauro; Zagaglia, Carlo; Gambara, Guido; Palombi, Fioretta; De Cesaris, Paola; Ziparo, Elio; Palamara, Anna T.; Riccioli, Anna

    2016-01-01

    Adherent/invasive Escherichia coli (AIEC) strains have recently been receiving increased attention because they are more prevalent and persistent in the intestine of Crohn's disease (CD) patients than in healthy subjects. Since AIEC strains show a high percentage of similarity to extraintestinal pathogenic E. coli (ExPEC), neonatal meningitis-associated E. coli (NMEC), and uropathogenic E. coli (UPEC) strains, here we compared AIEC strain LF82 with a UPEC isolate (strain EC73) to assess whether LF82 would be able to infect prostate cells as an extraintestinal target. The virulence phenotypes of both strains were determined by using the RWPE-1 prostate cell line. The results obtained indicated that LF82 and EC73 are able to adhere to, invade, and survive within prostate epithelial cells. Invasion was confirmed by immunofluorescence and electron microscopy. Moreover, cytochalasin D and colchicine strongly inhibited bacterial uptake of both strains, indicating the involvement of actin microfilaments and microtubules in host cell invasion. Moreover, both strains belong to phylogenetic group B2 and are strong biofilm producers. In silico analysis reveals that LF82 shares with UPEC strains several virulence factors: namely, type 1 pili, the group II capsule, the vacuolating autotransporter toxin, four iron uptake systems, and the pathogenic island (PAI). Furthermore, compared to EC73, LF82 induces in RWPE-1 cells a marked increase of phosphorylation of mitogen-activated protein kinases (MAPKs) and of NF-κB already by 5 min postinfection, thus inducing a strong inflammatory response. Our in vitro data support the hypothesis that AIEC strains might play a role in prostatitis, and, by exploiting host-cell signaling pathways controlling the innate immune response, likely facilitate bacterial multiplication and dissemination within the male genitourinary tract. PMID:27600504

  10. Detection of ionospheric perturbations associated with Japanese earthquakes on the basis of reception of LF transmitter signals on the satellite DEMETER

    Directory of Open Access Journals (Sweden)

    F. Muto

    2008-02-01

    Full Text Available There have been recently reported a lot of electromagnetic phenomena associated with earthquakes (EQs. Among these, the ground-based reception of subionospheric waves from VLF/LF transmitters, is recognized as a promising tool to investigate the ionospheric perturbations associated with EQs. This paper deals with the corresponding whistler-mode signals in the upper ionosphere from those VLF/LF transmitters, which is the counterpart of subionospheric signals. The whistler-mode VLF/LF transmitter signals are detected on board the French satellite, DEMETER launched on 29 June 2004. We have chosen several large Japanese EQs including the Miyagi-oki EQ (16 August 2005; M=7.2, depth=36 km, and the target transmitter is a Japanese LF transmitter (JJY whose transmitter frequency is 40 kHz. Due to large longitudinal separation of each satellite orbit (2500 km, we have to adopt a statistical analysis over a rather long period (such as 3 weeks or one month to have reliable data set. By analyzing the spatial distribution of JJY signal intensity (in the form of signal to noise ratio SNR during a period of 4 months including the Miyagi-oki EQ, we have found significant changes in the intensity; generally the SNR is significantly depleted before the EQ, which is considered to be a precursory ionospheric signature of the EQ. This abnormal effect is reasonably explained in terms of either (1 enhanced absorption of whistler-mode LF signals in the lower ionosphere due to the lowering of the lower ionosphere, or (2 nonlinear wave-wave scattering. Finally, this analysis suggests an important role of satellite observation in the study of lithosphere-atmosphere-ionosphere coupling.

  11. EAF-LF/VD-HCC工艺生产管线钢洁净度的分析%Analysis of Cleanliness of Pipeline Steel Produced by EAF-LF/VD-HCC Process

    Institute of Scientific and Technical Information of China (English)

    彭其春; 李源源; 左著; 汤敬华; 吕士忠

    2006-01-01

    针对衡阳钢管集团公司"EAF-LF/VD-HCC"管线钢生产工艺,采用示踪剂示踪、系统取样、综合分析的方法,对LF处理前后、VD处理后、中间包、铸坯和轧管中wT[O],w[N],显微夹杂物和大型夹杂物的变化及分布进行了系统研究.研究结果表明,精炼过程脱氧幅度为28.0×10-6,精炼处理后到轧管过程钢中wT[O]大幅度增加;VD后到中间包过程钢水吸氮最严重,为12.3×10-6.精炼过程去除夹杂的效果比较明显,处理前后显微夹杂物分别为10.85个/mm2和8.93个/mm2,大型夹杂物分别为65.74 mg/10 kg和24.50 mg/10kg,精炼过程夹杂物改性效果不明显.

  12. Chemical composition and antibacterial activity of the essential oil of Drimys granadensis L.f. leaves from Colombia.

    Science.gov (United States)

    Gaviria, Mauricio; Quijano, Clara; Pino, Jorge; Madriñán, Santiago

    2011-03-01

    Drimys granadensis L.f., a native plant from Colombia, has been included in the Vademecum de Plantas Medicinales de Colombia by the Instituto de Investigación de Recursos Biológicos Alexander von Humboldt, due to its widespread use in the folk medicine for the treatment of gastrointestinal ailments. The chemical composition of the essential oil of Drimys granadensis obtained by hydrodistillation of the leaves was analyzed by GC and GC/MS analyses. A total of 85 components were identified, with the major compounds germacrene D (1, 14.7%), sclarene (9.5%), a-cadinol (7.3%), longiborneol acetate (2, 6.3%), drimenol (4.2%), (Z)-β-ocimene (3, 4.2%), a-pinene (4, 3.2%), and β-elemene (5, 2.7%). The essential oil was also tested against eight bacteria using the Kirby-Bauer disk-diffusion method. Most of the Gram-positive bacteria tested were susceptible to the D. granadensis essential oil, while the Gram-negative bacteria tested were not.

  13. ISSR markers for analysis of molecular diversity and genetic structure of Indian teak (Tectona grandis L.f. populations

    Directory of Open Access Journals (Sweden)

    S.A. Ansari

    2012-05-01

    Full Text Available Inter simple sequence repeats (ISSR constitute a powerful dominantDNA molecular marker system used for diversity analysis, which isindispensable for making estimates of genetic base and demarcation of populations for undertaking conservation and improvement program of forest tree species. Twenty nine populations of teak (Tectona grandis L.f. were collected from central and peninsular India for analysis of genetic diversity and structure. Genomic DNA from ten randomly selected individuals of each population was extracted and amplified using five ISSR primers(UBC-801, 834, 880, 899 and 900. The primers showed 100% polymorphism. UBC-900 recorded the highest Nei’s genetic diversity (0.32 to 0.40and UBC-899 had the highest Shannon’s Information Index (0.49 to 0.59. AMOVA revealed a very high intra-population genetic diversity (91%, in comparison to inter-population genetic diversity among states (6.17% and within states (2.77% which were also indirectly confirmed by large standard deviations associated with genetic diversity estimates for individual population, as well as poor bootstrapping values for most of the cluster nodes. However, UPGMA dendrogram revealed several clusters, with populationsfrom central India being present almost in each cluster, makinggroups with populations of adjoining states and distant states. Nevertheless,the cluster analysis distinguished the drier teak populations of central India from the moist teak populations of south India, which was also confirmed by Principle Coordinate Analysis. The findings advocates the need not only for enhancing selection intensity for large number of plus trees, but also for laying out more number of in situ conservation plots within natural populations of each cluster for germplasm conservation of teak aimed at improving the teak productivity and quality in future.

  14. VLF/LF EM emissions as main precursor of earthquakes and their searching possibilities for Georgian s/a region

    Science.gov (United States)

    Kachakhidze, Manana; Kachakhidze, Nino

    2016-04-01

    Authors of abstract have created work which offers model of earth electromagnetic emissions generation detected in the process of earthquake preparation on the basis of electrodynamics. The model gives qualitative explanation of a mechanism of generation of electromagnetic waves emitted in the earthquake preparation period. Besides, scheme of the methodology of earthquake forecasting is created based on avalanche-like unstable model of fault formation and an analogous model of electromagnetic contour, synthesis of which, is rather harmonious. According to the authors of the work electromagnetic emissions in radiodiapason is more universal and reliable than other anomalous variations of various geophysical phenomena in earthquake preparation period; Besides, VLF/LF electromagnetic emissions might be declared as the main precursor of earthquake because it might turn out very useful with the view of prediction of large (M ≥5) inland earthquakes and to govern processes going on in lithosphere-atmosphere - ionosphere coupling (LAIC) system. Since the other geophysical phenomena, which may accompany earthquake preparation process and expose themselves several months, weeks or days prior to earthquakes are less informative with the view of earthquake forecasting, it is admissible to consider them as earthquake indicators. Physical mechanisms of mentioned phenomena are explained on the basis of the model of generation of electromagnetic emissions detected before earthquake, where a process of earthquake preparation and its realization are considered taking into account distributed and conservative systems properties. Up to these days electromagnetic emissions detection network did not exist in Georgia. European colleagues helped us (Prof. Dr. PF Biagi, Prof. Dr. Aydın BÜYÜKSARAÇ) and made possible the installation of a receiver. We are going to develop network and put our share in searching of earthquakes problem. Participation in conference is supported by financial

  15. Further study on the role of atmospheric gravity waves on the seismo-ionospheric perturbations as detected by subionospheric VLF/LF propagation

    Directory of Open Access Journals (Sweden)

    F. Muto

    2009-07-01

    Full Text Available As the target earthquake we have taken a huge earthquake (EQ named Miyagi-oki earthquake on 16 August 2005 (with magnitude of 7.2 and we have analyzed the 4 month period including the date of this EQ. In addition to our previous analysis on the nighttime average amplitude (trend and nighttime fluctuation, we have proposed the use of fluctuation power spectra in the frequency rage of atmospheric gravity waves (period=10 min to 100 min as a third parameter of subionospheric VLF/LF propagation characteristics. Then it is found that this third parameter would be of additional importance in confirming the presence of seismo-ionospheric perturbations. Finally, we have discovered an important role of lunar tidal effect in the VLF/LF data, which appears one and two months before this large EQ.

  16. 酒钢SPCC钢的LF精炼脱硫%Desulfurization of LF Refining Process for SPCC in JISCO

    Institute of Scientific and Technical Information of China (English)

    程子建; 郭靖; 程树森; 成东全

    2012-01-01

    通过热力学分析,建立了硫分配比与硫容量的关系,用热力学软件FactSage计算渣中Al2O3活度,用KTH模型计算渣的硫容量,对SPCC(一般用冷轧碳素钢薄板坯钢带)两个浇次10炉钢水在LF进站和出站时取钢、渣样以及测氧和温度,通过分析钢样和渣样成分以及生产检测数据,分析了温度、炉渣成分和钢水成分对LF精炼脱硫的影响规律.定义了硫分配比对钢液中溶解氧活度的急剧变化区(a[O]<4×10 6),在该区内硫分配比对钢液中溶解氧活度十分敏感,钢液中氧活度的增大导致硫分配比的迅速减小,温度升高,a[O]升高,不仅抵消了升温对脱硫反应轻微的促进作用,反而使硫分配比随温度升高而减小.LF精炼过程Al-O反应未达渣-钢平衡,实际[O]活度介于平衡计算值与Al2O3活度为1的计算值之间,故渣钢硫分配比也介于二者之间.精炼渣二元碱度升高则硫分配比增加,wCaO/wAl2O3在1.6~2.0时脱硫效果较好,硫分配比并不随[Al]s含量的增加而增大,所以用增加W[Al]s来脱硫效果并不明显.钢中夹杂铝(W[Al]1-W[Al]s)降低到10×10-6以下,硫分配比明显升高.%The relationship between sulfide partition ratio and sulfur capacity was established based on thermody-namic analysis, and the activity of Al2O3 in refining slag was calculated by applying thermodynamic software Fact-Sage and sulfide capacity was estimated by KTH model. Furthermore, ten steel and slag samples were taken at the start and end of ladle furnace (LF) refining for Steel Plate Cold Commercial (SPCC) in Jiuquan Iron and Steel Corporation (JISCO) , and at the same time the oxygen activity and temperature were measured using a oxygen probe. The influences of temperature and composition of refining slag and liquid steel on desulfurization at LF for SPCC were analyzed by both experimental data and thermodynamic calculated results. A rapid change region for sulfur partition ratio to dissolved

  17. Anaerobic decomposition of a native and an exotic submersed macrophyte in two tropical reservoirs

    Directory of Open Access Journals (Sweden)

    WA Chiba de Castro

    Full Text Available Some aquatic plants have fast metabolism and growth, even at sub-optimal conditions, and become dominant in lentic environments such as large reservoirs, altering the nutrient cycle and impairing their environmental quality. There is great need in the knowledge impact processes of invasive species in aquatic environments, among the major, those related to the decomposition. This study evaluated the anaerobic decomposition of invasive submerged macrophytes Egeria densa Planch, native, and Hydrilla verticillata (L.f. Royle, exotic in Porto Primavera and Jupiá reservoirs, Paraná basin. We evaluated the decay of organic matter, humification degree of the leached material, electrical conductivity and pH of the decomposition process. Mathematical models were utilised to describe the decomposition patterns over time. Both species showed the same heterogeneous pattern of decay of organic matter and carbon mineralisation. The models of carbon mineralisation, compared with the experimentally obtained data presented were adequate. Both species show no significant differences in the decomposition processes. Incubations of both species presented rapid t ½ for POC mineralisation and low DOC mineralisation.

  18. Heavy metals in water, sediments and wetland plants in an aquatic ecosystem of tropical industrial region, India.

    Science.gov (United States)

    Rai, Prabhat Kumar

    2009-11-01

    Concentrations of heavy metals (Cu, Cr, Fe, Pb, Zn, Hg, Ni, and Cd) and macronutrients (Mn) were measured in industrial effluents, water, bottom sediments, and wetland plants from a reservoir, Govind Ballabh (G.B.) Pant Sagar, in Singrauli Industrial region, India. The discharge point of a thermal power plant, a coal mine, and chlor-alkali effluent into the G.B. Pant Sagar were selected as sampling sites with one reference site in order to compare the findings. The concentrations of heavy metals in filtered water, sieved sediment samples (0.4-63 microm), and wetland plants were determined with particle-induced X-ray emission. The collected plants were Aponogeton natans, L. Engl. & Krause, Cyperus rotundus, L., Hydrilla verticillata, (L.f.) Royle, Ipomoea aquatica, Forssk., Marsilea quadrifolia, L., Potamogeton pectinatus, L., Eichhornia crassipes, (Mart.) Solms Monogr., Lemna minor, L., Spirodela polyrhiza (L.) Schleid. Linnaea, Azolla pinnata, R.Br., Vallisneria spiralis, L., and Polygonum amphibium, L. In general, metal concentration showed a significant positive correlation between industrial effluent, lake water, and lake sediment (p macrophytes for pollution monitoring.

  19. Opportunities for Phytoremediation and Bioindication of Arsenic Contaminated Water Using a Submerged Aquatic Plant:Vallisneria natans (lour.) Hara.

    Science.gov (United States)

    Chen, Guoliang; Liu, Xingmei; Brookes, Philip C; Xu, Jianming

    2015-01-01

    The identification of plants with high arsenic hyperaccumulating efficiency from water is required to ensure the successful application of phytoremediation technology. Five dominant submerged plant species (Vallisneria natans (Lour.) Hara., Potamageton crispus L., Myriophyllum spicatum L., Ceratophyllum demersum L. and Hydrilla verticillata (L.f.) Royle) in China were used to determine their potential to remove As from contaminated water. V. natans had the highest accumulation of As among them. The characteristics of As accumulation, transformation and the effect of phosphate on As accumulation in V. natans were then further studied. The growth of V. natans was not inhibited even when the As concentration reached 2.0 mg L(-1). After 21 d of As treatment, the bioconcentration factor (BCF) reached 1300. The As concentration in the environment and exposure time are major factors controlling the As concentration in V. natans. After being absorbed, As(V) is efficiently reduced to As(III) in plants. The synthesis of non-enzymic antioxidants may play an important role under As stress and increase As detoxication. In addition, As(V) uptake by V. natans was negatively correlated with phosphate (P) uptake when P was sufficiently supplied. As(V) is probably taken up via P transporters in V. natans.

  20. Assessment of TD-DFT and LF-DFT for study of d - d transitions in first row transition metal hexaaqua complexes.

    Science.gov (United States)

    Vlahović, Filip; Perić, Marko; Gruden-Pavlović, Maja; Zlatar, Matija

    2015-06-07

    Herein, we present the systematic, comparative computational study of the d - d transitions in a series of first row transition metal hexaaqua complexes, [M(H2O)6](n+) (M(2+/3+) = V (2+/3+), Cr(2+/3+), Mn(2+/3+), Fe(2+/3+), Co(2+/3+), Ni(2+)) by the means of Time-dependent Density Functional Theory (TD-DFT) and Ligand Field Density Functional Theory (LF-DFT). Influence of various exchange-correlation (XC) approximations have been studied, and results have been compared to the experimental transition energies, as well as, to the previous high-level ab initio calculations. TD-DFT gives satisfactory results in the cases of d(2), d(4), and low-spin d(6) complexes, but fails in the cases when transitions depend only on the ligand field splitting, and for states with strong character of double excitation. LF-DFT, as a non-empirical approach to the ligand field theory, takes into account in a balanced way both dynamic and non-dynamic correlation effects and hence accurately describes the multiplets of transition metal complexes, even in difficult cases such as sextet-quartet splitting in d(5) complexes. Use of the XC functionals designed for the accurate description of the spin-state splitting, e.g., OPBE, OPBE0, or SSB-D, is found to be crucial for proper prediction of the spin-forbidden excitations by LF-DFT. It is shown that LF-DFT is a valuable alternative to both TD-DFT and ab initio methods.

  1. Poliakrilonitril Nanolifler İçerisinde Altıgen Kobalt Sülfür Nanoplaka Sentezi

    Directory of Open Access Journals (Sweden)

    Yakup AYKUT

    2013-12-01

    Full Text Available Son yıllardaki yeni nano üretim ve karakterizasyon tekniklerindeki gelişmeler mevcut birçok malzemenin nano boyutta üretilmesine imkân sağlamış ve üretilen nanomalzemelerin cihazlarda kullanımıyla cihazların verimliliklerinde artışlara imkân sağlamıştır. İstenen birden fazla özelliğin eldesi ise nano hetero yapıların üretilmesiyle mümkündür. Yapılan bu çalışmamızda elektro çekim yöntemiyle kobalt klorür/poliakrilonitril (CoCl2/PAN kompozit nanolifler üretilmiş ve üretilen liflerin hidrojen sülfüre (H2S karşı olan tepkisi araştırılmıştır. CoCl2/PAN nanoliflerin H2S ile muamelesinde kobalt tuzunun yapısındaki kobalt iyonları çökelerek nanolifler içerisinde altıgen şekilde kobalt bileşenli nanoplakaların oluştuğu gözlemlenmiştir. Üretilen kompozit nanoliflerin morfolojik özellikleri taramalı elektron mikroskobuyla (SEM ile elementel analizleri ise X-ray fotoelektron spektra (XPS tekniğiyle incelenmiştir. Elde edilen bulgularla CoCl2/PAN nanoliflerle toksik bir kimyasal olan H2S in tespiti ve uzaklaştırılmasının mümkün olmasının yanı sıra nanolif içerisinde fonksiyonel başka bir nano yapının üretilebileceği sonucuna varılmıştır.

  2. Investigation of Diospyros Kaki L.f husk extracts as corrosion inhibitors and bactericide in oil field.

    Science.gov (United States)

    Zhang, Jie; Song, Yingpan; Su, Huijun; Zhang, Li; Chen, Gang; Zhao, Jingrui

    2013-07-01

    Hydrochloric acid is used in oil-well acidizing commonly for improving the crude oil production of the low-permeable reservoirs, while it is a great challenge for the metal instruments involved in the acidification. Developing natural products as oilfield chemicals is a straight way to find less expensive, green and eco-friendly materials. The great plant resources in Qin-ling and Ba-shan Mountain Area of Shannxi Province enable the investigating of new green oil field chemicals. Diospyros Kaki L.f (persimmon), a famous fruit tree is widely planted in Qin-ling and Ba-shan Mountain Area of Shaanxi Province. It has been found that the crude persimmon extracts are complex mixtures containing vitamins, p-coumaric acid, gallic acid, catechin, flavonoids, carotenoids and condensed tannin and so on, which indicates the extracts of persimmon husk suitable to be used as green and eco-friendly corrosion inhibitors. Extracts of persimmon husk were investigated, by using weight loss and potentiodynamic polarisation techniques, as green and eco-friendly corrosion inhibitors of Q235A steel in 1M HCl. The inhibition efficiency of the extracts varied with extract concentration from 10 to 1,000 mg/L. There are some synergistic effects between the extracts and KI, KSCN and HMTA. Potentiodynamic polarization studies indicate that extracts are mixed-type inhibitors. Besides, the extracts were screened for antibacterial activity against oil field microorganisms, and they showed good to moderate activity against SRB, IB and TGB. The inhibition efficiency of the extracts varied with extract concentration from 10 to 1,000 mg/L, and the highest reaches to 65.1% with the con concentration of 1,000 mg/L WE. KI, KSCN and HMTA they can enhance the IE of WE effectively to 97.3% at most, but not effective for KI and KSCN to AE. Tafel polarisation measurements indicate the extracts behave as mixed type inhibitor. Investigation of the antibacterial activity against oil field microorganism showed

  3. Development and Validation of a Fast and Optimized Screening Method for Enhanced Production of Secondary Metabolites Using the Marine Scopulariopsis brevicaulis Strain LF580 Producing Anti-Cancer Active Scopularide A and B

    OpenAIRE

    Kramer, Annemarie; Paun, Linda; Imhoff, Johannes F.; Kempken, Frank; Labes, Antje

    2014-01-01

    Natural compounds from marine fungi are an excellent source for the discovery and development of new drug leads. The distinct activity profiles of the two cyclodepsipeptides scopularide A and B against cancer cell lines set their marine producer strain Scopulariopsis brevicaulis LF580 into the focus of the EU project MARINE FUNGI. One of the main goals was the development of a sustainable biotechnological production process for these compounds. The secondary metabolite production of strain LF...

  4. Improving Coverage and Compliance in Mass Drug Administration for the Elimination of LF in Two 'Endgame' Districts in Indonesia Using Micronarrative Surveys.

    Science.gov (United States)

    Krentel, Alison; Damayanti, Rita; Titaley, Christiana Rialine; Suharno, Nugroho; Bradley, Mark; Lynam, Timothy

    2016-11-01

    As the Global Programme to Eliminate Lymphatic Filariasis (LF) approaches its 2020 goal, an increasing number of districts will enter the endgame phase where drug coverage rates from mass drug administration (MDA) are used to assess whether MDA can be stopped. As reported, the gap between reported and actual drug coverage in some contexts has overestimated the true rates, thus causing premature administration of transmission assessment surveys (TAS) that detect ongoing LF transmission. In these cases, districts must continue with additional rounds of MDA. Two districts in Indonesia (Agam District, Depok City) fit this criteria-one had not met the pre-TAS criteria and the other, had not passed the TAS criteria. In both cases, the district health teams needed insight into their drug delivery programs in order to improve drug coverage in the subsequent MDA rounds. To inform the subsequent MDA round, a micronarrative survey tool was developed to capture community members' experience with MDA and the social realm where drug delivery and compliance occur. A baseline survey was implemented after the 2013 MDA in endemic communities in both districts using the EPI sampling criteria (n = 806). Compliance in the last MDA was associated with perceived importance of the LF drugs for health (psurvey was performed after the 2014 MDA using the same sampling criteria (n = 811). Reported compliance in the last MDA improved in both districts from 57% to 77% (psurveys were shown to be a valid and effective tool to detect operational issues within MDA programs. District health staff felt ownership of the results, implementing feasible changes to their programs that resulted in significant improvements to coverage and compliance in the subsequent MDA. This kind of implementation research using a micronarrative survey tool could benefit underperforming MDA programs as well as other disease control programs where a deeper understanding is needed to improve healthcare delivery.

  5. PHENOTYPIC CHARACTERIZATION OF FRUIT FROM MAURITIA FLEXUOSA L.F. (ARECACEAE “AGUAJE” OF NINE COMMUNITIES OF DATEM DEL MARAÑON - LORETO REGION, PERUVIAN AMAZON.

    Directory of Open Access Journals (Sweden)

    Sissy Alvarez

    2012-12-01

    Full Text Available The aim of this study was to characterize phenotypically, through morphological and physicochemical variables, fruits of nine natural populations of Mauritia flexuosa L.f. (Arecaceae of DATEM del Marañón located in the Loreto region of Peru. To achieve the goal twelve variables were analyzed. Three principal components explained 91.22 % of the total variability. In the dendrogram with an Euclidean distance of 0.95, three population groups were visualized. Group I expressed the highest concentration of â-carotene (p0.10. This research will be useful for the selection of aguaje germplasm in Peru.

  6. The combined study of the middle atmosphere meteorological parameters and lower ionosphere dynamics over Europe by means of remote VLF-LF measurements

    Science.gov (United States)

    Zetzer, J. I.; Lyakhov, A.; Yakymenko, K.

    2012-12-01

    The results of comprehensive analysis of long-term records of VLF-LF European transmitters by means of Mikhnevo geophysical observatory (Institute of Geospheres Dynamics), SID station A118 (France) and a number of AWESOM receivers are presented. In the previous study (A.A.Egoshin et al., Izvestiya, Physics of the Solid Earth, 2012, Vol. 48, No. 3, pp. 275-286) the results obtained under the minimum solar activity were presented that have shown the link between the lower ionosphere parameters and meteorological fields of the middle atmosphere. The current study expands the evidence to the increased solar activity level as well as the number of receivers, thus allowing more dense coverage of the Europe by the radio paths. Middle atmosphere data under study were provided by the EOS Aura Microwave Limb Sounder. These asynoptic data, in turn, were processed by the space-time spectral analysis on various pressure levels for various window lengths. The results are presented for the spatial structure of wave-like perturbations in the VLF-LF signal strength, which result from the lower ionosphere disturbances on various radio paths. Special short-windowed space-time study was evaluated for the periods of anomalous temporal behavior of the VLF-LF signals and the results of the altitude-latitude mode structures of the geopotential height, neutral temperature, water and ozone constituents are presented. It is shown that the spatial properties of the lower ionosphere can vary significantly at relatively small scale. Moreover, the altitude-latitude mode structures do not coincide in space as well as in time, thus, complicating the lower ionosphere response to the meteorological variations of the middle atmosphere. The analysis of all assembled data proves two main points. At first, it is possible to evaluate synoptic long-term monitoring of the middle atmosphere via the lower ionosphere perturbations as seen by VLF-LF receivers. At second, the theoretical models of the lower

  7. Antibacterial properties of hLf1-11 peptide onto titanium surfaces: a comparison study between silanization and surface initiated polymerization.

    Science.gov (United States)

    Godoy-Gallardo, Maria; Mas-Moruno, Carlos; Yu, Kai; Manero, José M; Gil, Francisco J; Kizhakkedathu, Jayachandran N; Rodriguez, Daniel

    2015-02-09

    Dental implant failure can be associated with infections that develop into peri-implantitis. In order to reduce biofilm formation, several strategies focusing on the use of antimicrobial peptides (AMPs) have been studied. To covalently immobilize these molecules onto metallic substrates, several techniques have been developed, including silanization and polymer brush prepared by surface-initiated atom transfer radical polymerization (ATRP), with varied peptide binding yield and antibacterial performance. The aim of the present study was to compare the efficiency of these methods to immobilize the lactoferrin-derived hLf1-11 antibacterial peptide onto titanium, and evaluate their antibacterial activity in vitro. Smooth titanium samples were coated with hLf1-11 peptide under three different conditions: silanization with 3-aminopropyltriethoxysilane (APTES), and polymer brush based coatings with two different silanes. Peptide presence was determined by X-ray photoelectron spectroscopy, and the mechanical stability of the coatings was studied under ultrasonication. The LDH assays confirmed that HFFs viability and proliferation were no affected by the treatments. The in vitro antibacterial properties of the modified surfaces were tested with two oral strains (Streptococcus sanguinis and Lactobacillus salivarius) showing an outstanding reduction. A higher decrease in bacterial attachment was noticed when samples were modified by ATRP methods compared to silanization. This effect is likely due to the capacity to immobilize more peptide on the surfaces using polymer brushes and the nonfouling nature of polymer PDMA segment.

  8. High concentration of human lactoferrin in milk of rhLf-transgenic cows relieves signs of bovine experimental Staphylococcus chromogenes intramammary infection.

    Science.gov (United States)

    Simojoki, Heli; Hyvönen, Paula; Orro, Toomas; Pyörälä, Satu

    2010-08-15

    Six transgenic cows producing recombinant human lactoferrin (rhLf) in their milk and five normal cows at the same lactation stage were experimentally infected with Staphylococcus chromogenes to study the effect of a high concentration of lactoferrin in milk. Coagulase-negative staphylococci such as S. chromogenes have become very common as agents causing mild or subclinical mastitis. All transgenic cows became infected but showed no clinical signs, unlike the control cows, which developed mild clinical mastitis. Transgenic cows eliminated bacteria faster from the quarters than did the controls. Local clinical signs were milder, and the inflammatory reaction assessed by NAGase activity in the milk and by the concentration of milk amyloid A was lower in the transgenic cows. The mild response probably reflected the rapid elimination of bacteria. The milk concentration of rhLf remained constant throughout the study period, but the total concentration of bovine lactoferrin in the milk peaked in both groups at 46h post-challenge. Three cows, all in the control group, exhibited systemic acute phase response as increased concentrations of serum amyloid A in the blood circulation. Transgenic cows with a high concentration of human lactoferrin in their milk seemed to be protected from clinical disease and from prolonged inflammatory reaction, but not from experimental intramammary infection induced by S. chromogenes.

  9. A Proof of Concept to Bridge the Gap between Mass Spectrometry Imaging, Protein Identification and Relative Quantitation: MSI~LC-MS/MS-LF

    Science.gov (United States)

    Théron, Laëtitia; Centeno, Delphine; Coudy-Gandilhon, Cécile; Pujos-Guillot, Estelle; Astruc, Thierry; Rémond, Didier; Barthelemy, Jean-Claude; Roche, Frédéric; Feasson, Léonard; Hébraud, Michel; Béchet, Daniel; Chambon, Christophe

    2016-01-01

    Mass spectrometry imaging (MSI) is a powerful tool to visualize the spatial distribution of molecules on a tissue section. The main limitation of MALDI-MSI of proteins is the lack of direct identification. Therefore, this study focuses on a MSI~LC-MS/MS-LF workflow to link the results from MALDI-MSI with potential peak identification and label-free quantitation, using only one tissue section. At first, we studied the impact of matrix deposition and laser ablation on protein extraction from the tissue section. Then, we did a back-correlation of the m/z of the proteins detected by MALDI-MSI to those identified by label-free quantitation. This allowed us to compare the label-free quantitation of proteins obtained in LC-MS/MS with the peak intensities observed in MALDI-MSI. We managed to link identification to nine peaks observed by MALDI-MSI. The results showed that the MSI~LC-MS/MS-LF workflow (i) allowed us to study a representative muscle proteome compared to a classical bottom-up workflow; and (ii) was sparsely impacted by matrix deposition and laser ablation. This workflow, performed as a proof-of-concept, suggests that a single tissue section can be used to perform MALDI-MSI and protein extraction, identification, and relative quantitation. PMID:28248242

  10. A Proof of Concept to Bridge the Gap between Mass Spectrometry Imaging, Protein Identification and Relative Quantitation: MSI~LC-MS/MS-LF.

    Science.gov (United States)

    Théron, Laëtitia; Centeno, Delphine; Coudy-Gandilhon, Cécile; Pujos-Guillot, Estelle; Astruc, Thierry; Rémond, Didier; Barthelemy, Jean-Claude; Roche, Frédéric; Feasson, Léonard; Hébraud, Michel; Béchet, Daniel; Chambon, Christophe

    2016-10-26

    Mass spectrometry imaging (MSI) is a powerful tool to visualize the spatial distribution of molecules on a tissue section. The main limitation of MALDI-MSI of proteins is the lack of direct identification. Therefore, this study focuses on a MSI~LC-MS/MS-LF workflow to link the results from MALDI-MSI with potential peak identification and label-free quantitation, using only one tissue section. At first, we studied the impact of matrix deposition and laser ablation on protein extraction from the tissue section. Then, we did a back-correlation of the m/z of the proteins detected by MALDI-MSI to those identified by label-free quantitation. This allowed us to compare the label-free quantitation of proteins obtained in LC-MS/MS with the peak intensities observed in MALDI-MSI. We managed to link identification to nine peaks observed by MALDI-MSI. The results showed that the MSI~LC-MS/MS-LF workflow (i) allowed us to study a representative muscle proteome compared to a classical bottom-up workflow; and (ii) was sparsely impacted by matrix deposition and laser ablation. This workflow, performed as a proof-of-concept, suggests that a single tissue section can be used to perform MALDI-MSI and protein extraction, identification, and relative quantitation.

  11. A Proof of Concept to Bridge the Gap between Mass Spectrometry Imaging, Protein Identification and Relative Quantitation: MSI~LC-MS/MS-LF

    Directory of Open Access Journals (Sweden)

    Laëtitia Théron

    2016-10-01

    Full Text Available Mass spectrometry imaging (MSI is a powerful tool to visualize the spatial distribution of molecules on a tissue section. The main limitation of MALDI-MSI of proteins is the lack of direct identification. Therefore, this study focuses on a MSI~LC-MS/MS-LF workflow to link the results from MALDI-MSI with potential peak identification and label-free quantitation, using only one tissue section. At first, we studied the impact of matrix deposition and laser ablation on protein extraction from the tissue section. Then, we did a back-correlation of the m/z of the proteins detected by MALDI-MSI to those identified by label-free quantitation. This allowed us to compare the label-free quantitation of proteins obtained in LC-MS/MS with the peak intensities observed in MALDI-MSI. We managed to link identification to nine peaks observed by MALDI-MSI. The results showed that the MSI~LC-MS/MS-LF workflow (i allowed us to study a representative muscle proteome compared to a classical bottom-up workflow; and (ii was sparsely impacted by matrix deposition and laser ablation. This workflow, performed as a proof-of-concept, suggests that a single tissue section can be used to perform MALDI-MSI and protein extraction, identification, and relative quantitation.

  12. Temporal trends and effects of diversity on occurrence of exotic macrophytes in a large reservoir

    Science.gov (United States)

    Thomaz, Sidinei Magela; Carvalho, Priscilla; Mormul, Roger Paulo; Ferreira, Fernando Alves; Silveira, Márcio José; Michelan, Thaísa Sala

    2009-09-01

    Two exotic invasive macrophyte species (the emergent Urochloa subquadripara - tenner-grass - and the submersed Hydrilla verticillata - hydrilla) were investigated in a large sub-tropical reservoir. We analyzed their occurrences over an extended period and tested the hypothesis that macrophyte richness decreases their invasibility. The alternative hypothesis that the occurrence of these exotics is affected by fetch and underwater radiation (important determinants of macrophyte assemblage composition in this reservoir) was also tested. Incidence data (presence/absence) was obtained over 9.5 years at 235 stations. Logistic regression was applied to test whether the likelihood of occurrence of these two species was affected by macrophyte richness, fetch or underwater radiation. Tenner-grass was recorded at a high frequency and quickly recovered from disturbances caused by water drawdown. In contrast, H. verticillata was first recorded in 3 sites in January 2007, but it spread quickly, reaching 30.5% of the sites 19 months later. The main channel of the Paraná River was the main source of propagules for this species. The likelihood of occurrence of tenner-grass was positively affected by macrophyte richness but negatively affected by fetch. Thus, wave disturbance is probably more important than diversity in preventing invasion by this species. Hydrilla, by contrast, was negatively affected by macrophyte richness and positively affected by fetch and underwater radiation. Although this result might indicate that macrophyte diversity prevents hydrilla invasion, this is probably not true because hydrilla colonized deeper sites where few species of plant exist. Resistance to disturbances caused by water drawdown (tenner-grass) and waves (hydrilla) as well as persistency of tenner-grass and fast spread of hydrilla make these exotic species a cause for concern because of their potential impacts on water uses and maintenance of diversity.

  13. Crowdsourcing a Spatial Temporal Study of Low Frequency (LF) Propagation Effects Due to a Total Solar Eclipse: Engaging Students and Citizens in STEM

    Science.gov (United States)

    Lumsden, N. A.; Lukes, L.; Nelson, J.; Liles, W. C.; Kerby, K. C.; Crowov, F.; Rockway, J.

    2015-12-01

    The first experiments to study the effects of a solar eclipse on radio wave propagation were done in 1912 utilizing Low Frequency (LF; 30 - 300 kHz) radio waves at a handful of sites across Europe before any theory of the ionosphere had been confirmed and even before the word "ionosphere" existed. In the 1920s, a large cooperative experiment was promoted in the U.S. by Scientific American magazine. They collected over 2000 reports of AM broadcast stations from throughout the U.S. Unfortunately, many of the submissions were unusable because they lacked critical information such as date, time or location. We propose to use the 2017 solar eclipse over the continental U.S. to conduct the first wide-area LF propagation study. To perform this study, we plan to crowdsource the collection of the data by engaging student groups, citizens, and the scientific community. The tools for the different collection stations will consist of a simple homemade antenna, a simple receiver to convert the radio frequency (RF) signals to audio frequencies and a smart phone app. By using the time, date and location features of the smart phone, the problems experienced in the Scientific American experiment will be minimized. By crowdsourcing the observation sites, a number of different short, medium and long-paths studies can be obtained as the total eclipse crosses the continental U.S. The transmitter for this experiment will be WWVB located near Fort Collins, Colorado on 60.000 kHz. This is a U.S. frequency standard that is operated by NIST and transmits time codes. A second frequency, 55.500 kHz transmitted by a LF station in Dixon, CA is also being considered for this experiment. We will present an overall strategy for recruiting participants/crowdsourcing the RF collections during the 2017 total solar eclipse. Preliminary coverage calculations will be presented for WWVB and Dixon, as well as path loss calculations that can be expected during the solar eclipse condition. We will also

  14. Ionospheric turbulence from ground-based and satellite VLF/LF transmitter signal observations for the Simushir earthquake (November 15, 2006

    Directory of Open Access Journals (Sweden)

    Pier Francesco Biagi

    2012-04-01

    Full Text Available

    Signals from very low frequency (VLF/ low frequency (LF transmitters recorded on the ground station at Petropavlovsk-Kamchatsky and on board the French DEMETER satellite were analyzed for the Simushir earthquake (M 8.3; November 15, 2006. The period of analysis was from October 1, 2006, to January 31, 2007. The ground and satellite data were processed by a method based on the difference between the real signal at night-time and the model signal. The model for the ground observations was the monthly averaged signal amplitudes and phases, as calculated for the quiet days of every month. For the satellite data, a two-dimensional model of the signal distribution over the selected area was constructed. Preseismic effects were found several days before the earthquake, in both the ground and satellite observations.

     

  15. 气候变化和种子萌发特性对轮叶马先蒿种群扩张的影响%Impacts of climatic changes as well as seed germination characteristics on the population expansion of Pedicularis verticillata

    Institute of Scientific and Technical Information of China (English)

    隋晓琳; 李爱荣; 管开云

    2013-01-01

    轮叶马先蒿(Pedicularis verticillata)是一种根部半寄生性杂草,在新疆巴音布鲁克草原迅速蔓延,严重危害了当地畜牧业的利用和发展。通过分析巴音布鲁克草原近30年的气候变化并结合轮叶马先蒿种子的萌发特性和传播特点,探讨气候因素对近些年来轮叶马先蒿种群在巴音布鲁克草原迅速扩张的影响。结果显示:1)1980-2010年新疆巴音布鲁克草原轮叶马先蒿生长季内的日平均温,最高温,最低温均表现出增高趋势,且气候倾向率在0.3℃(10 a)左右;此外马先蒿生长季内的有效积温和年积温也有显著的增长,增幅分别为48.755℃(10 a)和61.469℃(10 a);生长季内的平均降雨量和年均降水量也出现了明显的增多,每10年的增幅在20 mm以上;说明巴音布鲁克草原的气候朝着“暖湿化”的方向发展。2)轮叶马先蒿种子具有循环休眠的现象,属条件休眠状态。而这个状态主要受温度条件的变化所影响,萌发率随温度升高而显著提高。室温储藏和赤霉素处理下轮叶马先蒿种子在3/16℃和5/20℃这2个变温条件下萌发率最高,可达50%以上;而湿冷层积处理中种子的萌发率虽然也表现为随温度的升高而提高,但5/20℃时的最大萌发率仅为(44.44±2.94)%。3)巴音布鲁克草原气候的“暖湿化”变化有利于轮叶马先蒿种子的萌发和扩散,这可能是导致轮叶马先蒿在巴音布鲁克草原迅速蔓延的主要原因之一。%Pedicularis verticillata is a kind of root hemiparasite, which has recently been expanding in Bayanbulak Grassland of Xinjiang, and reducing productivity of grassland. Hence, threatening the utilization and development of local animal husbandry industry. In this research, we focused on the patterns of climatic change in past 30 years and the effect of temperature on seed germination. We then analyzed the reasons for population

  16. A Sulfide Capacity Prediction Model of CaO-SiO2-MgO-FeO-MnO-Al2O3 Slags during the LF Refining Process Based on the Ion and Molecule Coexistence Theory

    Science.gov (United States)

    Yang, Xue-Min; Zhang, Meng; Shi, Cheng-Bin; Chai, Guo-Ming; Zhang, Jian

    2012-04-01

    A sulfide capacity prediction model of CaO-SiO2-MgO-FeO-MnO-Al2O3 ladle furnace (LF) refining slags has been developed based on the ion and molecule coexistence theory (IMCT). The predicted sulfide capacity of the LF refining slags has better accuracy than the measured sulfide capacity of the slags at the middle and final stages during the LF refining process. Increasing slag binary basicity, optical basicity, and the Mannesmann index can lead to an increase of the predicted sulfide capacity for the LF refining slags as well as to an increase of the sulfur distribution ratio between the slags and molten steel at the middle and final stages during the LF refining process. The calculated equilibrium mole numbers, mass action concentrations of structural units or ion couples, rather than mass percentages of components, are recommended to represent the slag composition for correlating with the sulfide capacity of the slags. The developed sulfide capacity IMCT model can calculate not only the total sulfide capacity of the slags but also the respective sulfide capacity of free CaO, MgO, FeO, and MnO in the slags. The comprehensive contribution of the combined ion couples (Ca2+ + O2-) and (Mn2+ + O2-) on the desulfurization reactions accounts for 96.23 pct; meanwhile, the average contribution of the ion couple (Fe2+ + O2-) and (Mg2+ + O2-) only has a negligible contribution as 3.13 pct and 0.25 pct during the LF refining process, respectively. The oxygen activity of bulk molten steel in LF is controlled by the [Al]-[O] equilibrium, and the oxygen activity of molten steel at the slag-metal interface is controlled by the (FeO)-[O] equilibrium. The ratio of the oxygen activity of molten steel at the slag-metal interface to the oxygen activity of bulk molten steel will decrease from 37 to 5 at the initial stage, and further decrease from 28 to 4 at the middle stage, but will maintain at a reliable constant as 5 to 14 at the final stage during the LF refining process. The

  17. Xylitol Processing from Corn Cobs Hydrolysates by Isolated and Encapsulated Candida tropicalis LF04 from Soil%驯化和胶囊包裹的假丝酵母LF04发酵玉米芯水解液生产木糖醇

    Institute of Scientific and Technical Information of China (English)

    马美湖; 杨涛; 周慧; 杨素芳

    2007-01-01

    玉米芯的酸水解液是木糖醇生产的重要原料,但是该水解液中含有糠醛、酚类等对后续微生物发酵有毒害作用的化合物.本研究从土壤中分离了一株似假丝酵母LF01,通过驯化和微胶囊包裹来提高其对水解液的抗性.结果表明通过多次驯化并进行包裹的假丝酵母LF04能在玉米芯水解液中不经任何脱毒处理发酵木糖生产木糖醇.在pH5.5溶氧为0.15vvm的条件下发酵88h,木糖转化率为76%,木糖醇浓度达61.768g/L.远高于其出发菌株.该结果表明采用该方法有望用于木糖醇的工业化生产.%The sulfuric acid hydrolysates of corn cob fiber is an important raw material for xylitol production. But the hydrolysates contain furfural and phenolic compounds and others, which are toxic to microorginasims during subsequent fermentation. In this study, new microorganisms Candida tropicalis LF01 were isolated from soil. Adaptation and encapsulation were carried out to enhance the inhibitors resistance in corn cob hydrolysate. The results indicated that the isolated and encapsulated strains Candida tropicalis LF04 could ferment xylose to xylitol in condensed corn cob hydrolysate without any detoxification. 76% xylose was fermented after 88 h at pH5.5 with the aeration 0.15vvm, and xylitol produced more than 61.768 g/L. The xylitol yield rate is up to 0.83 g/g, much higher than that of both the parent strains and the only isolated strain. The results also demonstrated that yeast treated with this method has the potential to be applied to xylitol industrial production.

  18. Species and biogeochemical cycles of organic phosphorus in sediments from a river with different aquatic plants located in Huaihe River Watershed, China.

    Science.gov (United States)

    Yuan, He Zhong; Pan, Wei; Ren, Li Jun; Liu, Eeng Feng; Shen, Ji; Geng, Qi Fang; An, Shu Qing

    2015-01-01

    The results of phosphorus fractionation in the sediments from a contaminated river containing different aquatic plants, analyzed by solution 31P-NMR for Organic Phosphorus, showed that the concentration of Inorganic Phosphorus dominated in all species and Organic Phosphorus accounted for over 20% of Total Phosphorus. In general, orthophosphate was dominant in all the sampling sites. The proportion of Organic Phosphorus accounting for the Total Phosphorus in the sediments with different plant decreased in the following order: Paspalum distichum>Typha orientalis>Hydrilla verticillata. Phosphorus-accumulation ability of Paspalum distichum was obviously stronger than Typha orientalis and Hydrilla verticillata. The Organic Phosphorus was in aquatic plants dominated by humic-associated P (Hu-P), which converted to Inorganic Ohosphorus more significantly in submerged plants than in emerged plants. The sediment dominated by Paspalum distichum abundantly accumulated Organic Phosphorus in the orthophosphate monoester fraction. The degradation and mineralization of orthophosphate monoester was the important source of high Inorganic Phosphorus concentration and net primary productivity in Suoxu River. The Organic Phosphorus derived from Typha orientalis and Hydrilla verticillata was dramatically converted to Inorganic Phosphorus when the environmental factors varied.

  19. Ionospheric turbulence from TEC variations and VLF/LF transmitter signal observations before and during the destructive seismic activity of August and October 2016 in Central Italy

    Science.gov (United States)

    Contadakis, Michael E.; Arabelos, Demetrios N.; Vergos, George; Spatala, Spyrous; Skeberis, Christos; Xenos, Tomas D.; Biagi, Pierfrancesco; Scordilis, Emmanuel M.

    2017-04-01

    In this paper we investigate the ionospheric turbulence from TEC variations and VLF/LF transmitter signal observations before and during the disastrous seismic activity of August and October 2016 in Central Italy . The Total Electron Content (TEC) data of 8 Global Positioning System (GPS) stations of the EUREF network, which are being provided by IONOLAB (Turkey), were analysed using Discrete Fourier Analysis in order to investigate the TEC variations (Contadakis et al. 2009, Contadakis et al. 2012, Contadakis et al. 2015). The data acquired for VLF/LF signal observations are from the receiver of Thessaloniki(40.59N, 22,78E), Greece (Skeberis et al. 2015) which monitor the VLF/LF transmitters of the International Network for Frontier Research on Earthquake Precursors (INFREP). A method of normalization according to the distance between the receiver and the transmitter is applied on the above data and then they are processed by the Hilbert Huang Transform (HHT) to produce the corresponding spectra for visual analysis. The results of this investigation indicate that the High- Frequency limit fo, of the ionospheric turbulence content, increases as the site and the moment of the earthquake occurrence is approaching, pointing to the earthquake locus. In accordence ,the analyzed data from the receiver of INFREP network in Thessaloniki, Greece show that the signals from the two VLF European transmitters, Tavolara ( Italy) and Le Blanc (France), for wich the transmission path crosses the epicentral zones, indicate enhanced high frequency variations, the last ten days before the moment of the earthquake occurrence. We conclude that the LAIC mechanism through acoustic or gravity wave could explain this phenomenology. Reference Contadakis, M.E., Arabelos, D.N., Asteriadis, G., Spatalas, S.D., Pikridas, C. TEC variations over the Mediterranean during the seismic activity period of the last quarter of 2005 in the area of Greece, Nat. Hazards and Earth Syst. Sci., 8, 1267

  20. Evaluation of butachlor for control of some submerged macrophytes along with its impact on biotic components of freshwater system

    Directory of Open Access Journals (Sweden)

    A. Chattopadhyay, S. Adhikari, S. P. Adhikary, S. Ayyappan

    2006-04-01

    Full Text Available In this investigation, the efficacy of the herbicide butachlor, (N-butoxymethyl-2 chloro-21, 61 diethyl acetanilide was tested against few common submerged macrophytes namely Hydrilla (Hydrilla verticillata (L. Royale, Najas (Najas minor All., Nechamandra (Nechamandra alternifolia (Roxb. Thwaites and Ottelia (Ottelia alismoides (L. Pers. of freshwater fish ponds. Almost complete decay of Hydrilla, Nechamandra and Ottelia was achieved at 7.5 L of active ingredient/ha/m butachlor within 15 days while the herbicide showed no negative effect on Najas. However at the same concentration of butachlor, total mortality of zooplankton and water fern Azolla (Azolla caroliniana Lamarck occurred within seven days. In case of few freshwater fish species like Rohu (Labeo rohita, Channa (Channa punctatus, Anabas (Anabas testitudineus and Heteropneustes (Heteropneustes fossilis, total mortality occurred upto 90 days after application of the same dose of butachlor but fish survived beyond 120 days of herbicide application indicating degradation of the herbicides.

  1. 水生植物的生态敏感度研究%Ecological Sensitivity of Aquatic Plants

    Institute of Scientific and Technical Information of China (English)

    李宏文; Paul; K.Chien

    2001-01-01

    Throughy the study on the ecological effects of Cd and Zn onseveral aquatic plants,the ecological sensitivity of 5 species is determined.The results show that according to critical time when plants are injured by Cd and Zn,the order for ecological sensitivity of 5 species is:Nymphoides peltatum>Hydrilla verticillata>Potamogeton malaianus>Spirodela polyrhiza>Alternanthera philoxeroides.The results also show that on basis of catalase activity of aquatic plants,the order for ecological sensitivity of 5 species is:Alternanthera philoxeroides>Spirodela polyrhiza≈Potamogeton malaianus>Hydrilla verticillata>Nymphoides peltatum.It is evident that the pollution-durablty Potamogeton malaianus is higher than for Nymphoides peltatum and Hydrilla verticillata,pollution-sensible species

  2. Dendrophthoe falcata (L.f) Ettingsh (Neem mistletoe): A potent bioresource to fabricate silver nanoparticles for anticancer effect against human breast cancer cells (MCF-7)

    Science.gov (United States)

    Sathishkumar, Gnanasekar; Gobinath, Chandrakasan; Wilson, Arockiyasamy; Sivaramakrishnan, Sivaperumal

    2014-07-01

    Fabrication of metal nano scale particles through environmentally acceptable greener route has been focused with much interest in the present scenario. In this study aqueous leaf extract of mistletoe Dendrophthoe falcata (L.f) Ettingsh was successfully employed as a reducing and stabilizing agent to fabricate nanosilver particles (AgNPs) for biomedical applications. Various reactions conditions such as temperature, pH, concentration of metal ion, incubation time and stoichiometric proportion of the reaction mixture were optimized to attain narrow size range particles with maximum synthesis rate. Fabricated crystalline AgNPs with spherical structure (5-45 nm) were characterized with UV-Visible spectroscopy, Field emission scanning electron microscope (FESEM), High resolution transmission electron microscope (HRTEM) and Selected area diffraction pattern (SEAD). Further the fabricated AgNPs were studied for their stability and surface chemistry through Fourier transform infrared spectroscopy (FTIR), Energy dispersive X-ray spectroscopy (EDAX) and inductively coupled plasma optical emission spectroscopy (ICP-OES). Moreover, fabricated AgNPs and aqueous leaf extract were assessed for their cytotoxicity effect against human breast carcinoma cell line (MCF-7). It is concluded that colloidal AgNPs can be developed as an imminent candidature for cancer therapy.

  3. Variations of VLF/LF signals observed on the ground and satellite during a seismic activity in Japan region in May–June 2008

    Directory of Open Access Journals (Sweden)

    A. Rozhnoi

    2010-03-01

    Full Text Available Signals of two Japanese transmitters (22.2 kHz and 40 kHz recorded on the ground VLF/LF station in Petropavlovsk-Kamchatsky and on board the DEMETER French satellite have been analyzed during a seismic activity in Japan in May–June 2008. The period of analysis was from 18 April to 27 June. During this time two rather large earthquakes occurred in the north part of Honshu Island – 7 May (M=6.8 and 13 June (M=6.9. The ground and satellite data were processed by a method based on the difference between the real signal in nighttime and the model one. For ground observations a clear decrease in both signals has been found several days before the first earthquake. For the second earthquake anomalies were detected only in JJI signal. The epicenters of earthquakes were in reliable reception zone of 40 kHz signal on board the DEMETER. Signal enhancement above the seismic active region and significant signal intensity depletion in the magnetically conjugate area has been found for satellite observation before the first earthquake. Anomalies in satellite data coincide in time with those in the ground-based observation.

  4. Evaluation of the Toxic Effect from Eupatorium Microphyllum L.F. (Asteraceae Extracts on Aedes Aegypti Larvae (Diptera: Culicidae in Laboratory Conditions

    Directory of Open Access Journals (Sweden)

    Álvaro Rozo

    2008-07-01

    Full Text Available In the present work the toxic activity ofextracts of Eupatorium microphyllum L.F. wasevaluated on 4th instar larvae of the mosquitoAedes aegypti (Linneaus, under laboratoryconditions. Aqueous extracts were utilized inconcentrations of 500 mg L-1, 1,500 mg L-1 and2,500 mg L-1 and acetone in concentrations of10 mg L-1, 20 mg L-1, 30 mg L-1, 40 mg L-1and50 mg L-1. The bioassays were carried out fortriplicate each one with 20 larvae, exposedfor 24 hours to 150 mL of solution. In all thebioassays were employed control groups. Inthe evaluation of the acetone extracts, a negativecontrol was employed to avoid that themortality of the larvae to occur on account ofthe solvent. The Aqueous extracts showed lowmoderate action in the mortality of larvae, lessthan 20%. On the contrary, the action of theacetone extracts was observed to 10 and 20 mgL-1with 15% of mortality, while to 30 and 40mg L-1 were registered 22 to 38% of mortality.However, to 50 mg L-1 the mortality wasof 95.4% with highly significant statisticalresults. The concentrations of the acetone extractsshowed to be the most efficient for thecontrol of the mosquitoes selected. Both typesof extracts showed toxic effect in larvae of A.aegypti, nevertheless, greater effect in theacetone extracts was observed relating to theaqueous extracts of E. microphyllum, whichconstitutes a viable alternative in the search ofnew larvicides from composed natural.

  5. De Novo Assembly and Genome Analyses of the Marine-Derived Scopulariopsis brevicaulis Strain LF580 Unravels Life-Style Traits and Anticancerous Scopularide Biosynthetic Gene Cluster

    Science.gov (United States)

    Kumar, Abhishek; Henrissat, Bernard; Arvas, Mikko; Syed, Muhammad Fahad; Thieme, Nils; Benz, J. Philipp; Sørensen, Jens Laurids; Record, Eric; Pöggeler, Stefanie; Kempken, Frank

    2015-01-01

    The marine-derived Scopulariopsis brevicaulis strain LF580 produces scopularides A and B, which have anticancerous properties. We carried out genome sequencing using three next-generation DNA sequencing methods. De novo hybrid assembly yielded 621 scaffolds with a total size of 32.2 Mb and 16298 putative gene models. We identified a large non-ribosomal peptide synthetase gene (nrps1) and supporting pks2 gene in the same biosynthetic gene cluster. This cluster and the genes within the cluster are functionally active as confirmed by RNA-Seq. Characterization of carbohydrate-active enzymes and major facilitator superfamily (MFS)-type transporters lead to postulate S. brevicaulis originated from a soil fungus, which came into contact with the marine sponge Tethya aurantium. This marine sponge seems to provide shelter to this fungus and micro-environment suitable for its survival in the ocean. This study also builds the platform for further investigations of the role of life-style and secondary metabolites from S. brevicaulis. PMID:26505484

  6. De Novo Assembly and Genome Analyses of the Marine-Derived Scopulariopsis brevicaulis Strain LF580 Unravels Life-Style Traits and Anticancerous Scopularide Biosynthetic Gene Cluster.

    Science.gov (United States)

    Kumar, Abhishek; Henrissat, Bernard; Arvas, Mikko; Syed, Muhammad Fahad; Thieme, Nils; Benz, J Philipp; Sørensen, Jens Laurids; Record, Eric; Pöggeler, Stefanie; Kempken, Frank

    2015-01-01

    The marine-derived Scopulariopsis brevicaulis strain LF580 produces scopularides A and B, which have anticancerous properties. We carried out genome sequencing using three next-generation DNA sequencing methods. De novo hybrid assembly yielded 621 scaffolds with a total size of 32.2 Mb and 16298 putative gene models. We identified a large non-ribosomal peptide synthetase gene (nrps1) and supporting pks2 gene in the same biosynthetic gene cluster. This cluster and the genes within the cluster are functionally active as confirmed by RNA-Seq. Characterization of carbohydrate-active enzymes and major facilitator superfamily (MFS)-type transporters lead to postulate S. brevicaulis originated from a soil fungus, which came into contact with the marine sponge Tethya aurantium. This marine sponge seems to provide shelter to this fungus and micro-environment suitable for its survival in the ocean. This study also builds the platform for further investigations of the role of life-style and secondary metabolites from S. brevicaulis.

  7. Morphological parameters and genetic diversity of progenies from seed production areas and unimproved stands of teak (Tectona grandis L.f.) in India

    Institute of Scientific and Technical Information of China (English)

    N. Lyngdoh; Geeta Joshi; G. Ravikanth; R. Vasudeva; R. Uma Shaanker

    2013-01-01

    We compared morphological and genetic attributes of prog-eny obtained from three seed production areas (SPAs) and three corre-sponding unimproved stands (UIS) of teak (Tectona grandis L.f) at three broad teak growing regions in the state of Karnataka, India. In general, seed morphological parameters such as seed weight, seed size and seed emptiness were significantly superior in SPAs compared to UISs. Seed germination percentage was also higher in seeds from SPAs. Seedling performance measured at two monthly intervals for six months was ob-served to be superior in SPAs at two of the three regions compared. Although the genetic diversity of progeny of SPAs was lower than UIS at all regions, the values were moderately high. Germination percent was positively correlated with fruit weight and kernel weight per seed, while diversity indices were negatively correlated with fruit weight, fruit di-ameter and kernel weight per seed. SPAs proved important as a source of moderately improved planting material with no severe threat to the ge-netic diversity of future plantations.

  8. On the solar cycle dependence of winds and planetary waves as seen from mid-latitude D1 LF mesopause region wind measurements

    Directory of Open Access Journals (Sweden)

    C. Jacobi

    Full Text Available At the Collm Observatory of the University of Leipzig LF D1 low-frequency total reflection night-time wind measurements have been carried out continuously for more than two decades. Using a multiple regression analysis to derive prevailing winds, tides and the quasi-2-day wave from the half-hourly mean values of the horizontal wind components, monthly mean values of mesopause wind parameters are obtained that can be analysed with respect to long-term trends and influences of solar variability. The response of the prevailing wind to the 11-year solar cycle differs throughout the year. While in winter no significant correlation between the zonal prevailing wind and solar activity is found, in spring and summer a negative correlation between the TWC can be seen from the measurements. This is connected with stronger vertical gradients of the zonal prevailing wind during solar maximum than during solar minimum. Since the amplitude of the quasi-2-day wave is dependent on the zonal mean wind vertical gradient, this is connected with a positive correlation between solar activity and quasi-two-day wave activity.

    Key words. Meteorology and atmospheric dynamics · Middle atmosphere dynamics Multiple regression analysis Quasi-2-day wave

  9. Finite Element Simulation of Bulge Forming Process of the LF2M Anti-rust Aluminum Pipe Coupling Based on Damage Development Model%基于损伤理论的LF2M管接头胀形工艺模拟

    Institute of Scientific and Technical Information of China (English)

    王志刚; 李付国; 何敏

    2009-01-01

    通过对LF2M进行拉伸试验,应用弹性模量下降法测定了Lemaitre损伤模型中的三个材料参数εD,εR,Dc.在损伤演化模型的基础上,基于ABAQUS/Explicit平台开发了用户材料子程序VUMAT,以此为基础模拟了航空高压管接头胀形工艺过程,模拟结果表明,胀形导管的损伤部位与实际情况相符,并且最大损伤变量远远小于临界损伤值,证明高压管接头胀形工艺设计可靠.

  10. Crescimento e desenvolvimento da planta daninha capim-camalote Growth and development of the weed itchgrass (Rottboelia exaltata L.f.

    Directory of Open Access Journals (Sweden)

    Saul Jorge Pinto de Carvalho

    2005-01-01

    Full Text Available O capim-camalote (Rottboelia exaltata L.f. é uma planta daninha com rápida disseminação nas áreas canavieiras do Brasil, causando significativas perdas de produtividade na cultura. Assim, esta pesquisa teve por objetivo avaliar o crescimento, o desenvolvimento vegetativo e a capacidade reprodutiva do capim-camalote, como mecanismo de melhoria do manejo a ser adotado. O experimento foi desenvolvido em casa de vegetação da ESALQ/USP, entre julho e outubro de 2004, quando foram realizadas 14 avaliações periódicas de crescimento, determinando-se: massa fresca e seca (total, parte aérea e raízes, área foliar e fenologia das plantas. Realizou-se a contagem do número de rácemos florais de 16 plantas e do número de sementes de 100 rácemos após florescimento. Pôde-se observar um rápido crescimento inicial das plantas, de tal forma que o início do florescimento ocorreu aos 49 dias após a semeadura. No fim do ciclo, verificaram-se nas plantas valores próximos a 120 g, 25 g e 1.600 cm² de massa fresca total, massa seca total e área foliar, respectivamente. Na ocasião das contagens, as plantas haviam emitido, em média, o total de 163 rácemos com 12 sementes cada uma, o que corresponde a mais de 2.000 sementes por planta. Pelos resultados alcançados verifica-se elevada capacidade de crescimento e habilidade reprodutiva do capim-camalote, explicando as razões que fazem dessa planta daninha um novo problema em potencial para a agricultura brasileira.Itchgrass (Rottboelia exaltata L.f. is a weed that has presented fast dissemination in sugar-cane fields in Brazil, causing significant yield losses to the crop. Therefore, this research had the objective of evaluating the growth, vegetative development and reproductive capacity of the species, as a mechanism of improving the management to be adopted. The experiment was conducted in the greenhouse of ESALQ/USP, Piracicaba, State of São Paulo, Brazil, during the months of July to

  11. AFM Morphology Study of Si1-Y GeY:H Films Deposited by LF PE CVD from Silane-Germane with Different

    Energy Technology Data Exchange (ETDEWEB)

    Sanchez, L; Kosarev, A

    2005-03-28

    The morphology of Si{sub 1-Y} Ge{sub Y}:H films in the range of Y=0.23 to 0.9 has been studied by AFM. The films were deposited by Low Frequency (LF) PE CVD at substrate temperature T{sub s}=300 C and discharge frequency f=110 kHz from silane+germane mixture with and without, Ar and H{sub 2} dilution. The films were deposited on silicon and glass substrates. AFM images were taken and analyzed for 2 x 2 mm{sup 2} area. All the images demonstrated ''grain'' like structure, which was characterized by the height distribution function F(H) average roughness , standard height deviation Rq, lateral correlation length L{sub c} area distribution function F(s), mean grain area , diameter distribution function F(d), and mean grain diameter . The roughness of the films monotonically increases with Y for all dilutions, but more significantly in the films deposited without dilution. L{sub c} continuously grows with Y in the films deposited without dilution, while more complex behavior L{sub c}(Y) is observed in the films deposited with H- or Ar dilution. The sharpness of F(H) characterized by curtosis {gamma} depends on dilution and the sharpest F(H) are for the films deposited with Ar ({gamma}=5.30,Y=0.23) and without dilution ({gamma}=4.3, Y=0.45). Isothermal annealing caused increase of , L{sub c} in the films deposited with H- and Ar dilutions, while in the films prepared without dilution the behavior was more complex, depending on the substrates. Significant narrowing of the height distribution was observed in the films deposited with H dilution or without dilution.

  12. Identity-based High-performance thin Layer Chromatography Fingerprinting Profile and Tumor Inhibitory Potential of Anisochilus carnosus (L.f.) wall Against Ehrlich Ascites Carcinoma

    Science.gov (United States)

    Gupta, Nilesh; Lobo, Richard; Kumar, Nimmy; Bhagat, Jay Kumar; Mathew, Jessy Elizabeth

    2015-01-01

    Context: Anisochilus carnosus (L.f.) wall belonging to the family Lamiaceae is a plant that is widely used in folk medicine for treating eczema, cold, cough, and fever. Objective: In the present study, we explored the anticancer potential of A. carnosus leaves against Ehrlich ascites carcinoma (EAC) and estimated the quantity of luteolin present in various extracts and fractions of A. carnosus by high-performance thin layer chromatography (HPTLC) fingerprinting. Materials and Methods: Various factors such as tumor volume, tumor cell viability, tumor weight, prolongation of lifespan, and hematological parameters were assessed. Result: We observed a significant lowering in tumor volume, tumor weight, and cell viability in EAC-induced mice following intervention with A. carnosus extracts. Also, there was a considerable prolongation of host lifespan and restoration of hematological parameters to almost normal levels with A. carnosus treatment. HPTLC fingerprinting of various extracts and fractions of A. carnosus along with luteolin as the reference standard revealed the occurrence of luteolin in all tested extracts and fractions of A. carnosus with the highest concentration being reported in the ethanol fraction. Conclusion: A. carnosus exhibits potent anti-tumor potential which can most likely be attributed to the occurrence of different phytochemicals such as phytosterols, terpenoids, and flavonoids in the plant. Further studies to isolate compounds from A. carnosus and understand the mechanism of anti-tumor activity would be worthwhile. SUMMARY EAC induced mice that received A. carnosus treatment exhibited significant reduction in tumor volume, tumor weight and tumor cell viability. Their life span was considerably prolonged. We detected luteolin in A. carnosus aqueous and ethanol extract using HPTLC. Hence, anticancer activity of A. carnosus can be partly attributed to the presence of luteolin. PMID:26929584

  13. Renealmia L.f.: aspectos botânicos, ecológicos, farmacológicos e agronômicos

    Directory of Open Access Journals (Sweden)

    R.R.B NEGRELLE

    2015-06-01

    Full Text Available RESUMO:Renealmia L.f. é um gênero de Zingiberaceae com inúmeros usos, entre eles: ornamental, medicinal e alimentício. Visando ressaltar a importância deste gênero como potencial fonte de recursos agroeconômicos apresenta-se esta revisão sobre aspectos botânicos, ecológicos, farmacológicos, e agronômicos. De um universo de 87 espécies formalmente inseridas em Renealmia, registrou-se indicação de uso popular para 18 destas. Em sua maior parte as indicações de uso estiveram associadas a R. alpinia, R. exaltata e R. guianensis englobando, principalmente, os usos ornamental, alimentício e medicinal. Dentre as 14 espécies com indicação de uso popular medicinal, registram-se pesquisas farmacológicas para apenas quatro: R. alpinia, R. exaltata, R. nicolaioides e R. thyrsoidea. Entretanto, estas pesquisas evidenciam um amplo espectro de bioatividade, com ênfase na ação anticancerígena e antiofídica, especialmente de R. alpinia. Apenas seis espécies foram avaliadas quanto a composição química (R. floribunda, R. guianensis, R. alpinia, R. chrysotricha, R. exaltata e R. nicolaioides, identificando-se perfil químico que corrobora a potencialidade anticancerígena e antiofídica para os representantes deste gênero. Evidenciou-se grande lacuna no que se refere ao conhecimento científico ou técnico para o cultivo de espécies de Renealmia. Isto pode se configurar num impedimento importante na utilização destas espécies como recurso econômico.

  14. The development of the International Network for Frontier Research on Earthquake Precursors (INFREP) by designing new analysing software and by setting up new recording locations of radio VLF/LF signals in Romania

    Science.gov (United States)

    Moldovan, Iren-Adelina; Petruta Constantin, Angela; Emilian Toader, Victorin; Toma-Danila, Dragos; Biagi, Pier Francesco; Maggipinto, Tommaso; Dolea, Paul; Septimiu Moldovan, Adrian

    2014-05-01

    Based on scientific evidences supporting the causality between earthquake preparatory stages, space weather and solar activity and different types of electromagnetic (EM) disturbances together with the benefit of having full access at ground and space based EM data, INFREP proposes a complex and cross correlated investigation of phenomena that occur in the coupled system Lithosphere-Atmosphere-Ionsophere in order to identify possible causes responsible for anomalous effects observed in the propagation characteristics of radio waves, especially at low (LF) and very low frequency (VLF). INFREP, a network of VLF (20-60 kHz) and LF (150-300 kHz) radio receivers, was put into operation in Europe in 2009, having as principal goal, the study of disturbances produced by the earthquakes on the propagation properties of these signals. The Romanian NIEP VLF / LF monitoring system consisting in a radio receiver -made by Elettronika S.R.L. (Italy) and provided by the Bari University- and the infrastructure that is necessary to record and transmit the collected data, is a part of the international initiative INFREP. The NIEP VLF / LF receiver installed in Romania was put into operation in February 2009 in Bucharest and relocated to the Black-Sea shore (Dobruja Seismologic Observatory) in December 2009. The first development of the Romanian EM monitoring system was needed because after changing the receiving site from Bucharest to Eforie we obtained unsatisfactory monitoring data, characterized by large fluctuations of the received signals' intensities. Trying to understand this behavior has led to the conclusion that the electric component of the electromagnetic field was possibly influenced by the local conditions. Starting from this observation we have run some tests and changed the vertical antenna with a loop-type antenna that is more appropriate in highly electric-field polluted environments. Since the amount of recorded data is huge, for streamlining the research process

  15. L-F001, a Multifunction ROCK Inhibitor Prevents 6-OHDA Induced Cell Death Through Activating Akt/GSK-3beta and Nrf2/HO-1 Signaling Pathway in PC12 Cells and Attenuates MPTP-Induced Dopamine Neuron Toxicity in Mice.

    Science.gov (United States)

    Luo, Liting; Chen, Jingkao; Su, Dan; Chen, Meihui; Luo, Bingling; Pi, Rongbiao; Wang, Lan; Shen, Wei; Wang, Rikang

    2017-02-01

    Amounting evidences demonstrated that Rho/Rho-associated kinase (ROCK) might be a novel target for the therapy of Parkinson's disease (PD). Recently, we synthesized L-F001 and revealed it was a potent ROCK inhibitor with multifunctional effects. Here we investigated the effects of L-F001 in PD models. We found that L-F001 potently attenuated 6-OHDA-induced cytotoxicity in PC12 cells and significantly decreased intracellular reactive oxygen species (ROS), prevented the 6-OHDA-induced decline of mitochondrial membrane potential and intracellular GSH levels. In addition, L-F001 increased Akt and GSK-3beta phosphorylation and induced the nuclear Nrf2 and HO-1 expression in a time- and concentration-dependent manner. Moreover, L-F001 restored the levels of p-Akt and p-GSK-3beta (Ser9) as well as HO-1 expression reduced by 6-OHDA. Those effects were blocked by the specific PI3K inhibitor, LY294002, indicating the involvement of Akt/GSK-3beta pathway in the neuroprotective effect of L-F001. In addition, L-F001 significantly attenuated the tyrosinehydroxylase immunoreactive cell loss in 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine (MPTP)-induced mice PD model. Together, our findings suggest that L-F001 prevents 6-OHDA-induced cell death through activating Akt/GSK-3beta and Nrf2/HO-1 signaling pathway and attenuates MPTP-induced dopaminergic neuron toxicity in mice. L-F001 might be a promising drug candidate for PD.

  16. Optimization of PRD drilling and completion fluid system for ZJ10 limestone reservoir of LF15-1 oilfield%LF15-1油田ZJ10灰岩储层PRD钻完井液体系优化研究

    Institute of Scientific and Technical Information of China (English)

    王晋玲; 赵峰; 唐洪明; 徐诗雨; 邢希金; 王珊

    2016-01-01

    LF15-1油田ZJ10灰岩储层为孔隙型储层,以中、低孔低渗为主,储层孔吼较小,裂缝不发育,钻完井液对储层存在不同程度的伤害.从储层基本特征、储层敏感性评价及目前所用钻井液性能评价着手,分析了储层潜在的损害因素,开展了储层保护钻完井液体系优选研究.基于选择性暂堵技术,得到了PRD钻完井液体系具体配方,建议在钻井液体系中加入0.7% PF-VIS和2.0% PF-FLO;在完井液体系中加入5% PF-JPC和2% CA101-4.动态损害实验评价表明,优选的PRD钻完井液体系对储层保护效果显著,液相侵入深度及表皮系数下降明显.

  17. Development and validation of a fast and optimized screening method for enhanced production of secondary metabolites using the marine Scopulariopsis brevicaulis strain LF580 producing anti-cancer active scopularide A and B.

    Directory of Open Access Journals (Sweden)

    Annemarie Kramer

    Full Text Available Natural compounds from marine fungi are an excellent source for the discovery and development of new drug leads. The distinct activity profiles of the two cyclodepsipeptides scopularide A and B against cancer cell lines set their marine producer strain Scopulariopsis brevicaulis LF580 into the focus of the EU project MARINE FUNGI. One of the main goals was the development of a sustainable biotechnological production process for these compounds. The secondary metabolite production of strain LF580 was optimized by random mutagenesis employing UV radiation. For a fast and reliable detection of the intracellular secondary metabolite production level, a miniaturized bioactivity-independent screening method was developed, as the random mutagenesis yielded a large number of mutants to be analysed quantitatively and none of the existing hyphenated bioassay-dependent screening systems could be applied. The method includes decreased cultivation volume, a fast extraction procedure as well as an optimized LC-MS analysis. We show that deviation could be specifically reduced at each step of the process: The measuring deviation during the analysis could be minimized to 5% and technical deviation occurring in the downstream part to 10-15%. Biological variation during the cultivation process still has the major influence on the overall variation. However, the approach led to a 10-fold reduction of time and similar effects on costs and effort compared to standard reference screening methods. The method was applied to screen the UV-mutants library of Scopulariopsis brevicaulis LF580. For validation purposes, the occurring variations in the miniaturized scale were compared to those in the classical Erlenmeyer flask scale. This proof of concept was performed using the wild type strain and 23 randomly selected mutant strains. One specific mutant strain with an enhanced production behavior could be obtained.

  18. Development and validation of a fast and optimized screening method for enhanced production of secondary metabolites using the marine Scopulariopsis brevicaulis strain LF580 producing anti-cancer active scopularide A and B.

    Science.gov (United States)

    Kramer, Annemarie; Paun, Linda; Imhoff, Johannes F; Kempken, Frank; Labes, Antje

    2014-01-01

    Natural compounds from marine fungi are an excellent source for the discovery and development of new drug leads. The distinct activity profiles of the two cyclodepsipeptides scopularide A and B against cancer cell lines set their marine producer strain Scopulariopsis brevicaulis LF580 into the focus of the EU project MARINE FUNGI. One of the main goals was the development of a sustainable biotechnological production process for these compounds. The secondary metabolite production of strain LF580 was optimized by random mutagenesis employing UV radiation. For a fast and reliable detection of the intracellular secondary metabolite production level, a miniaturized bioactivity-independent screening method was developed, as the random mutagenesis yielded a large number of mutants to be analysed quantitatively and none of the existing hyphenated bioassay-dependent screening systems could be applied. The method includes decreased cultivation volume, a fast extraction procedure as well as an optimized LC-MS analysis. We show that deviation could be specifically reduced at each step of the process: The measuring deviation during the analysis could be minimized to 5% and technical deviation occurring in the downstream part to 10-15%. Biological variation during the cultivation process still has the major influence on the overall variation. However, the approach led to a 10-fold reduction of time and similar effects on costs and effort compared to standard reference screening methods. The method was applied to screen the UV-mutants library of Scopulariopsis brevicaulis LF580. For validation purposes, the occurring variations in the miniaturized scale were compared to those in the classical Erlenmeyer flask scale. This proof of concept was performed using the wild type strain and 23 randomly selected mutant strains. One specific mutant strain with an enhanced production behavior could be obtained.

  19. Variación en la resistencia de clones de teca (Tectona grandis L.f.) ante la infección de la roya (Olivea tectonae Rac.) en Costa Rica

    OpenAIRE

    Arguedas-Gamboa, Marcela; Instituto Tecnológico de Costa Rica, Escuela de Ingeniería Forestal; Cartago, Costa Rica; Murillo-Gamboa, Olman; Instituto Tecnológico de Costa Rica, Escuela de Ingeniería Forestal; Cartago, Costa Rica; Ayuso, Federico; Expomaderas S.A.; Madrigal, Oscar; Expomaderas S.A.

    2012-01-01

    Se realizó una evaluación de resistencia de diferentes clones de teca (Tectona grandis L.f.) ante la infección de la roya (Olivea tectonae Rac.), en el jardín clonal de la empresa Expomaderas S.A., San Carlos, Costa Rica. Se evaluaron la incidencia clonal y la incidencia y severidad de la infección de 83 clones seleccionados de árboles plus en tres regiones geográficas de la zona norte del país: a) Los Chiles (San Emilio), b) Upala (Canalete), y c) El Concho (Pocosol, San Carlos) y Altamira d...

  20. 基于大小场景整合的遥感小目标检测算法%Small target detection algorithm of remote sensing data based on LF-SF integration

    Institute of Scientific and Technical Information of China (English)

    李敏; 范新南; 张学武; 张卓

    2013-01-01

    针对目前遥感图像小目标检测算法遇到的复杂背景建模困难、先验信息严重匮乏等问题,考虑到昆虫视觉系统在图像处理方面的优势,提出基于蝇视觉系统大小场景(LF-SF)整合的信息处理模式解决遥感图像存在复杂背景的目标检测.蝇视觉的LF-SF整合机理无需考虑图像背景的复杂度以及目标先验信息,在抑制复杂背景纹理特征的同时对稀有目标特征进行增强,最后通过加权融合实现目标检测.实验结果表明,本文算法在算法设计、处理速度以及检测精度均优于现有算法.%Aiming at the present problems of small target detection algorithm for remote sensing data,such as hard to model background feature and lack of prior information seriously,considering the advantages of insect vision on image processing,this paper proposes a parallel processing model to solve the problem of target detection of remote sensing under clutter background,which is inspired by the large field and small field (LF-SF) integration theory in fly vision system.Without background model and prior information,the theory of LF-SF integration can reduce the influence of backgrotmd feature,enhance the seldom target feature at the same time,and at last it uses weighted fusion to complete target detection.Experiment results show that the proposed method is better than the present algorithms in computation quantity,speed and accuracy of detection result.

  1. Ensayos de trabajabilidad de Tectona grandis L.f. de 11 años de edad proveniente de dos sitios de crecimiento en plantaciones del Atlántico y el Pacífico de Costa Rica

    OpenAIRE

    2012-01-01

    En este artículo se presentan los resultados de ensayos comparativos de trabajabilidad de la madera de teca (Tectona grandis L.f.) procedente de plantaciones forestales de 11 años de edad de dos regiones geográficas de Costa Rica (Atlántica y Pacífica). Tanto en la región Atlántica (Pococí) como en la región Pacífica (Parrita), se seleccionaron dos sitios, uno de mayor crecimiento (Sitio 1) y otro de menor crecimiento (Sito 2). Los ensayos incluyeron cepillado, lijado, taladrado y torneado. S...

  2. Gestión de la fertilidad de suelos y la nutrición de plantaciones de teca (Tectona grandis L.f.) en América Central

    OpenAIRE

    Fernández Moya, Jesús

    2014-01-01

    La teca (Tectona grandis L.f.) ha sido tradicionalmente considerada como una madera preciosa en los países del SE Asiático, de donde es originaria, pero durante las últimas décadas ha alcanzado especial relevancia en el sector internacional de las maderas tropicales duras de buena calidad. La especie ha sido ampliamente establecida en América Central, donde tiene una gran importancia socioeconómica, tanto por el impacto de las grandes empresas multinacionales que gestionan grandes plantacione...

  3. Improvements in the use of aquatic herbicides and establishment of future research directions

    Science.gov (United States)

    Getsinger, K.D.; Netherland, M.D.; Grue, C.E.; Koschnick, T.J.

    2008-01-01

    Peer-reviewed literature over the past 20 years identifies significant changes and improvements in chemical control strategies used to manage nuisance submersed vegetation. The invasive exotic plants hydrilla (Hydrilla verticillata L.f. Royle) and Eurasian watermilfoil (Myriophyllum spicatum L.) continue to spread and remain the plant species of greatest concern for aquatic resource managers at the national scale. Emerging exotic weeds of regional concern such as egeria (Egeria densa Planch.), curlyleaf pondweed (Potamogeton crispus L.), and hygrophila (Hygrophila polysperma (Roxb.) T. Anders), as well as native plants such as variable watermilfoil (Myriophyllum heterophyllum Michx), and cabomba (Cabomba caroliniana Gray) are invasive outside their home ranges. In addition, there is always the threat of new plant introductions such as African elodea (Lagarosiphon major (Ridley) Moss) or narrow-leaf anacharis (Egeria najas Planchon). The registration of the bleaching herbicide fluridone in the mid 1980s for whole-lake and large-scale management stimulated numerous lines of research involving reduction of use rates, plant selectivity, residue monitoring, and impacts on fisheries. In addition to numerous advances, the specificity of fluridone for a single plant enzyme led to the first documented case of herbicide resistance in aquatic plant management. The resistance of hydrilla to fluridone has stimulated a renewed interest by industry and others in the registration of alternative modes of action for aquatic use. These newer chemistries tend to be enzyme-specific compounds with favorable non-target toxicity profiles. Registration efforts have been facilitated by increased cooperation between key federal government agencies that have aquatic weed control and research responsibilities, and regulators within the U.S. Environmental Protection Agency (USEPA). We reviewed past and current research efforts to identify areas in need of further investigation and to establish

  4. LF-NMR study of effect the octadecylamine addition in the copolymerization process between acrylic acid and styrene monomers; Estudo por RMN de baixo campo do efeito da adicao de octadecilamina na copolimerizacao dos monomeros de acido acrilico e estireno

    Energy Technology Data Exchange (ETDEWEB)

    Pedroza, Oscar J.O.; Tavares, Maria I.B. [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil). Inst. de Macromoleculas Professora Eloisa Mano]. E-mail: oscarjop@ima.ufrj.br, mibt@ima.ufrj.br

    2005-07-01

    The copolymer content at least two monomer units that are the repetitive unities in the polymeric chains. The use of Low Field Nuclear Magnetic Resonance (LF-NMR), MARAN ultra 23, was applied to measure the proton spin-lattice relaxation time values. The process of copolymerization between the acrylic acid (A) and the styrene (S) monomers was studied with the addition of the octadecylamine (D) in the acrylic acid monomer. These materials were submitted at reflux by 24 hours. After this process the polymerization was carried out at room temperature. The values of the relaxation parameter are showed in Table 1. The co polymerizations between acrylic acid and styrene monomers were influenced by the octadecylamine addition. The results showed that an increase in the amine concentration promotes flexibility in the final material. This can be explained in terms of chains size after amine addition, which promotes an increasing in the free space among the polymer chains. (author)

  5. Association of the -33C/G OSF-2 and the 140A/G LF gene polymorphisms with the risk of chronic rhinosinusitis with nasal polyps in a Polish population.

    Science.gov (United States)

    Zielinska-Blizniewska, Hanna; Sitarek, Przemyslaw; Milonski, Jaroslaw; Dziki, Lukasz; Przybylowska, Karolina; Olszewski, Jurek; Majsterek, Ireneusz

    2012-05-01

    Nasal polyps are strongly associated with a risk of chronic rhinosinusitis development as well as other obstruction including asthma and allergy. The following study tested the association of the 140A/G polymorphism of lactoferine (LF) encoding gene and the -33C/G polymorphism of osteoblast-specific factor-2 (OSF-2) encoding gene with a risk of chronic rhinosinusitis with nasal polyps in a Polish population. One hundred ninety five patients of chronic rhinosinusitis with nasal polyps as well as 200 sex, age and ethnicity matched control subjects without chronic sinusitis and nasal polyps were enrolled in this study. Among the group of patients 63 subjects were diagnosed with allergy and 65 subjects with asthma, respectively. DNA was isolated from peripheral blood lymphocytes of patients as well as controls and gene polymorphisms were analyzed by restriction fragments length polymorphism polymerase chain reaction (RFLP-PCR). We reported that the 140A/G LF (OR 4.78; 95% CI 3.07-7.24), the -33C/G OSF-2 OR 3.48; 95% CI 2.19-5.52) and the -33G/G OSF-2 (OR 16.45; 95% CI 6.71-40.30) genotypes were associated with an increased risk of chronic rhinosinusitis with nasal polyps among analyzed group of patients. Moreover, the group of patients without allergy or asthma indicated the association of the -33C/G (OR 3.72; 95% CI 2.24-6.19 and OR 15.11; 95% CI 5.91-38.6) and -33G/G (OR 3.73; 95% CI 2.24-6.19 and OR 14.07; 95% CI 5.47-36.16) genotypes of the OSF-2 as wells as 140A/G (OR 3.89; 95% CI 2.40-6.31 and OR 3.62; 95% CI 2.45-5.34) genotype of OSF-2 with an increased risk of chronic rhinosinusitis with nasal polyps. Finally, it was also found that the selected group of patients with allergy or asthma indicated a very strong association of the -33C/G (OR 2.40; 95% CI 1.23-4.69 and OR 2.40; 95% CI 1.23-4.69, respectively) and -33G/G (OR 16.01; 95% CI 5.77-44.41 and OR 17.90; 95% CI 6.53-49.05, respectively) genotypes of the OSF-2 as wells as 140A/G (OR 3.22; 95% CI 1

  6. İstanbul-Beylerbeyi Sarayı’nda Coleoptera Türlerine Karşı Sülfüril Florit Kullanılarak Yapılan Fumigasyon Uygulaması

    OpenAIRE

    Yıldırım, Neşe; Taşkın, Hatice; KARAMAN, Rıfat

    2012-01-01

    ÖzetBeylerbeyi Sarayı’nın ahşap olan taşıyıcı sistem elemanlarında ve tarihi ahşap mobilyalarında, Coleoptera takımına ait, yaygın olarak Anobium punctatum (De Geer, 1774) (Coleoptera: Anobiidae), az miktarda Lyctus brunneus (Stephens, 1830) (Coleoptera: Lyctidae) ve Hylotrupes bajulus (Linnaeus, 1758) (Coleoptera: Cerambycidae)’un neden olduğu yoğun bir enfeksiyon tespit edilmiştir. Beylerbeyi Sarayı’ndaki ahşaplarda oluşan böcek enfeksiyonunu yok etmek için Türkiye’de ilk kez sülfüril flori...

  7. Multiplex PCR Detection of Exogenous Gene in hLF Transgenic Cow%转人乳铁蛋白基因奶牛多重PCR检测方法的研究

    Institute of Scientific and Technical Information of China (English)

    朱振营; 张书霞; 李风翠; 林祥梅; 吴绍强; 仇松寅; 李宁; 王建武; 薛振华; 刘建

    2011-01-01

    本研究旨在建立转基因奶牛多重PCR快速检测方法,为转基因动物及产品出入境检测技术平台的建立提供技术支持,并为转基因动物及产品检测技术标准的制定提供参考.根据牛物种特异性基因(mtDNA)设计奶牛内源基因引物,根据外源基因人乳铁蛋白基因(human lactoferrin,hLF)和新霉素磷酸转移酶基因(NPTⅡ)设计特异性引物,优化反应条件,建立转基因奶牛多重PCR检测方法.本方法敏感、快速、特异,一个反应可以检测多个基因片段,可有效用于转基因奶牛外源基因的检测.%For developing the typical testing standard of transgenic mammals and its derived food and the technical support for entry & exit animal quarantine, detection of the transgenic component in transgenic cow by multiplex PCR was developed in this study. The endogenous mtDNA gene specific for cow identification, exotic hLF gene,marker NPTⅡ gene were amplified according to different primers. The process of the multiplex PCR reaction was optimized. The system described herein represent simple, accurate, and sensitive detection methods in which only one reaction was necessary to detect multiple target sequences that can be reliably used for the identification of specific lines of transgenic cows.

  8. Rapid discrimination method of varieties of rice by using LF-NMR technology%基于低场核磁共振技术的大米品种快速鉴别

    Institute of Scientific and Technical Information of China (English)

    姜潮; 韩剑众

    2012-01-01

    Based on the Low Field-Nuclear Magnetic Resonance (LF-NMR)technology, a new method to discriminate varieties of rice was proposed.Rice samples of different producing areas were tested by the CPMG sequence of LF-NMR.Principal Component Analysis(PCA) method was used to analyze and process the sample data.The results showed that.Rice samples of different producing areas were distinguished effectively on the plot of PCA score.The new method proposed was capable to discriminate the varieties of rice,which provided a new method to discriminate rice varieties.%为探索大米无损检测技术,提出了一种基于低场核磁共振技术的快速、无损鉴别大米品种的新方法。以不同地域的大米为低场核磁共振检测对象,利用主成分分析法(PCA)分析处理Carr-Purcell-Meiboom-Gill(CPMG)序列的检测数据。实验结果表明,不同地域的大米在主成分得分图上可以得到很好的区分;说明所提出的方法具有很好的分类和鉴别作用,为大米的品种鉴别提供了一种新方法。

  9. Effets de lisière sur la productivité du teck (Tectona grandis L.f.: étude de cas des teckeraies privées du Sud-Bénin

    Directory of Open Access Journals (Sweden)

    Toyi, MS.

    2013-01-01

    Full Text Available Edge Effects on the Productivity of Teak (Tectona grandis L.f.: a Case Study of Private Teak Plantations in Southern Benin. The present study aims to improve the production of teak wood (Tectona grandis L.f. in private plantations in southern Benin through the application of a central concept in landscape ecology: the edge effect. As teak is an heliophilous species, the hypothesis of a higher wood production in edges was tested on the basis of the basal area. Sixty-two private teak plantations were investigated and 10,667 trees were measured. The stratified sampling scheme in three distinct parts for each plantation (the centre, the edge and the summits permitted to highlight the edge effect on wood production. For each part, a plot was installed and the diameter at breast height (dbh was measured for all trees. The leaf area between the edge and the centre of plantations was measured. Finally, the influence of the spatial configuration of plantations and the direction of each side of these plantations on the production of wood was tested. Results show that the edge effect on the production of teak wood affects four planting lines, the first presenting a production of 150% relative to the centre. We noticed a significant influence of the edge on the leaf area of about 218% relative to the centre. No influence of the direction of the sides of the plantation was observed. The shape of the plantations presents a significant influence on the wood production. These results permitted to propose a planting model included in an agroforestry system that optimizes the production of wood per area and having a succession of two planting lines interrupted by fields.

  10. 不同水生植物对水体中氮磷吸收去除效果的试验%Experiment of Absorption and Removal Efficiency for Nitrogen and Phosphorus with Several Different Hydrophytes

    Institute of Scientific and Technical Information of China (English)

    苏小东; 李艳; 原金海; 贾云

    2014-01-01

    研究了狐尾藻、水蕴草、轮叶黑藻、长叶久冠和铜线草等水生植物吸收水体中N、P的能力。结果表明狐尾藻、水蕴草、轮叶黑藻和长叶久冠对水体中的氮磷有较强的去除能力,且植物对水体中氮磷的去除率与植物质量之间呈相关关系;同时狐尾藻、水蕴草、轮叶黑藻和长叶久冠均有较好的环境适应性,而铜线草的环境适应性较差。由此推断可以利用狐尾藻、水蕴草、轮叶黑藻和长叶久冠水生植物在生态塘污水处理系统中构建水生植物系统来处理生活污水。%Several hydrophytes named Myriophyllum Verticillatum,Elodea densa (Planch. )Casp. ,Hydrilla Verticillata,Echinodorus Uruguayensis and Paspalum pospaloides(Michx. )Scribn were used to investigate the ability of removal of nitrogen and phosphor in the sewage. The results show that Myriophyllum Verticillatum,Elodea densa (Planch. )Casp. ,Hydrilla Verticillata and Echinodorus Uruguayensis have a remarkable effect on removal of N and P,and the removal rates of hydrophytes on N and P have correlation to weight of hydrophytes. Myriophyllum Verticillatum, Elodea densa (Planch. ) Casp. , Hydrilla Verticillata and Echinodorus Uruguayensis have good environment adaptability,by which hydrophytes-based treatment system for ecological pond sewage treatment system can be constructed.

  11. Does mechanical disturbance affect the performance and species composition of submerged macrophyte communities?

    Science.gov (United States)

    Zhang, Qian; Xu, Ying-Shou; Huang, Lin; Xue, Wei; Sun, Gong-Qi; Zhang, Ming-Xiang; Yu, Fei-Hai

    2014-01-01

    Submerged macrophyte communities are frequently subjected to disturbance of various frequency and strength. However, there is still little experimental evidence on how mechanical disturbance affects the performance and species composition of such plant communities. In a greenhouse experiment, we constructed wetland communities consisting of five co-occurring clonal submerged macrophyte species (Hydrilla verticillata, Elodea canadensis, Ceratophyllum demersum, Chara fragilis, and Myriophyllum spicatum) and subjected these communities to three mechanical disturbance regimes (no, moderate and strong disturbance). Strong mechanical disturbance greatly decreased overall biomass, number of shoot nodes and total shoot length, and increased species diversity (evenness) of the total community. It also substantially decreased the growth of the most abundant species (H. verticillata), but did not affect growth of the other four species. Our data reveal that strong disturbance can have different effects on different submerged macrophyte species and thus alters the performance and species composition of submerged macrophyte communities. PMID:24811826

  12. STUDY OF AQUATIC ANGIOSPERMIC PLANTS OF ANAND CITY, GUJARAT, INDIA

    Directory of Open Access Journals (Sweden)

    K. R. PATEL1 AND N. K. PATEL2

    2014-06-01

    Full Text Available The present study deals with the taxonomic study of Aquatic Angiosperms growing throughout the Anand city. The plants are listed along with their brief taxonomic account of each species with current nomenclature, vernacular name, family and uses. The  collected plants are systematically observed during present work, During my study I observed various aquatic angiospermic plants such as   Ceratophyllum demersum, Colocasia esculenta, Eichhornia crassipes, Ipomoea aquatica, Nymphoides indicum, Ludwigia repens, Polygonum orientale, Typha elephantina, Lemna perpusilla, Spirodella polyrrhiza, Xanthium indicum, Phyllanthus reticulatus, Cynodon dactylon, Hydrilla verticillata were very common. Whereas Nymphaea nouchali, Polygonum barbatum, Scirpus articulatus were very rare in the study area.

  13. Chromium accumulation in submerged aquatic plants treated with tannery effluent at Kanpur, India.

    Science.gov (United States)

    Gupta, Kiran; Gaumat, Sumati; Mishra, Kumkum

    2011-09-01

    Aquatic macrophytes have been widely studied because of their capability of absorbing contaminants from water and their subsequent use in biomonitoring. This study presents a comparison of Cr accumulating potential of submerged aquatic plants viz Vallisneria spiralis and Hydrilla verticillata. These plants were treated with various concentrations of treated tannery effluent collected from UASB, Jajmau, Kanpur under repeated exposure in controlled laboratory conditions in order to assess their maximum bioaccumulation potential. The maximum accumulation of 385.6 and 201.6 microg g(-1) dry weight was found in roots of V. spiralis and the whole plants of H. verticillata, respectively at 100% concentration after 9th day of effluent exposure. The chlorophyll and protein content of both species decreased with increase in effluent concentration and duration. At highest concentration and duration a maximum reduction of 67.4 and 62.66% in total chlorophyll content, 9.97 and 4.66% in carotenoid content and 62.66 and 59.36% in protein content was found in V. spiralis and H. verticillata respectively. Anatomical studies in both V. spiralis and H. verticillata was carried out to assess the effects of metal accumulation within the plants. Changes in the anatomical structures of both plants exhibits the capacity of these species to act as indicator of effluent toxicity. The high accumulation potential of Cr by both plants revealed their capability to remove pollutants from effluent.

  14. Establishing Research and Management Priorities for Monoecious Hydrilla

    Science.gov (United States)

    2014-01-01

    between the biotypes were successful and variations of these early tests are in use today (Ryan et al. 1991; Madeira et al. 2004; Rybicki et al. 2013...Management Society awarded a graduate student stipend to Dr. Rob Richardson at North Carolina State University (NCSU) to evaluate the phenology and...optimal treatment timing require additional information on tuber sprouting dynamics (e.g. plant phenology information described above would provide

  15. 无氟预熔LF精炼渣的开发与应用研究%Development and Application of LF Refining Pre-Melted Slag Without CaF2

    Institute of Scientific and Technical Information of China (English)

    潘贻芳; 凌遵峰; 王宝明; 李树庆; 王振峰

    2006-01-01

    为了避免有氟渣的氟污染问题,结合炉渣基础理论,设计开发出了CaO-Al2O3-MgO-SiO2系无氟精炼渣.精炼渣的工业应用表明,该渣能满足BOF-LF-CC工艺生产石油套管钢(37Mn5、34Mn5)的要求,钢管的实物质量达到美国石油行业API.5CT标准,并可使LF精炼操作时间由42 min缩短到35 min,为高效生产和快节奏奠定了基础.与常规有氟渣相比,避免了氟污染问题,且达到了脱氧、脱硫效果好,精炼初期成渣快、发泡埋弧效果好及包衬侵蚀较轻的目的.

  16. VARIAÇÃO BIOMÉTRICA DE DIÁSPOROS DE BURITI (Mauritia flexuosa L.f. – ARECACEAE EM VEREDAS EM DIFERENTES ESTÁGIOS DE CONSERVAÇÃO

    Directory of Open Access Journals (Sweden)

    Fabiano dos Santos Matos

    2014-01-01

    Full Text Available ‘Veredas’ (palm swamps are phytophysiognomies of the ‘Cerrado’ biome, where the palm tree Mauritia flexuosa Lf. - buriti predominates on the tree layer; this species is considered the key resource for many animals, that use it as shelter and source of food, and also by the man who uses their leaves and fruits as raw material and food. Although, they occur in environments protected by the environmental legislation, human actions have affected the populations of ‘buritis’, by means of the decrease of individuals caused by deforestation, siltation and drainage of these areas. The objective of this study was to evaluate the morphometric parameters of fruits and seeds of Mauritia flexuosa from six different ‘veredas’ at different conservation stages, located in the Environmental Protection Area of ‘Pandeiros’ River (north of Minas Gerais state. In total, 1,800 fruits and seeds were sampled, they were measured in length, width and thickness and, after that, they were weighed. To detect differences in morphometric measurements of fruits and seeds among the sampling areas, the analysis of variance was done. There were differences in morphometric measurements between the studied areas, in which 87.5% of the highest averages occurred in areas with the slightest anthropic interventions. This fact is possibly related to the impacts occurred, besides the genetic variations that may fall upon the individuals.

  17. 大气噪声对甚低频通信系统干扰仿真分析%Simulation and Analysis of Almospheric Noise Interference on VLF/LF Communication

    Institute of Scientific and Technical Information of China (English)

    施意; 张爽; 张昕

    2013-01-01

    甚低频通信又称为长波通信,具有传播距离远,水下传播,传播信号稳定、衰减小等特点,大气噪声会对甚低频通信系统产生严重影响,结合大气噪声经典模型和CCIR 322报告中多年的测量结果,对大气噪声干扰下的甚低频通信系统进行仿真,得出在不同强度的大气噪声干扰下,甚低频通信系统的误码率,验证了大气噪声中的主要为脉冲噪声对甚低频通信系统产生严重影响。%The very low frequency/low frequency communication systems althrough greatly influenced by atmospheric noise generally has the features of long propagation distance, stable propagation signal, low attenuation and underwater propagation. In combination of classical model of atmospheric noise and observational data from CCIR Report 322, the simulation on VLF/LF communication with atmospheric noise is done, and this simulation indicates that the impulse noise in the atmospheric noise has a serious impact on VLF communication system.

  18. Effectiveness of the two microorganisms Lactobacillus fermentum LF15 and Lactobacillus plantarum LP01, formulated in slow-release vaginal tablets, in women affected by bacterial vaginosis: a pilot study.

    Science.gov (United States)

    Vicariotto, Franco; Mogna, Luca; Del Piano, Mario

    2014-01-01

    Bacterial vaginosis (BV) is the most common reason for abnormal vaginal discharge in reproductive-age women and one of its most important causative agents is the gram-variable bacterium Gardnerella vaginalis. BV is not accompanied by significant local inflammation, whereas the "fishy odor" test is always positive. In contrast, aerobic vaginitis (AV) is predominantly associated with Escherichia coli, but Streptococcus agalactiae and Staphylococcus aureus are also involved. Standard treatment of BV consists of oral or intravaginal antibiotics, although these are unable to spontaneously restore normal flora characterized by a high concentration of lactobacilli. The main limitation is the inability to offer a long-term defensive barrier, thus facilitating relapses and recurrences. This study was undertaken firstly to assess the ability of selected lactobacilli to in vitro antagonize G. vaginalis to determine an association with a strain able to inhibit E. coli, thus identifying a possible use in AV. The second step of the study was to conduct a human pilot trial in women affected by BV using an association of the most promising and active bacteria. For this purpose, neutralized supernatants of individual lactobacilli were tested at percentages ranging from 0.5% to 4% to determine their ability to hinder the growth of G. vaginalis American Type Culture Collection 10231. The bacterium that was able to exert the strongest inhibition was subsequently tested with Lactobacillus plantarum LP01 in a human intervention, placebo-controlled, pilot trial involving 34 female subjects (aged between 18 and 50, mean 34.7±8.9, no menopausal women) diagnosed with BV. The 2 microorganisms Lactobacillus fermentum LF15 (DSM 26955) and L. plantarum LP01 (LMG P-21021) were delivered to the vagina by means of slow-release vaginal tablets, also containing 50 mg of tara gum. The amount of each strain was 400 million live cells per dose. The women were instructed to apply a vaginal tablet once

  19. Micropropagation of Clerodendrum phlomidis L.F.

    Directory of Open Access Journals (Sweden)

    Kher Mafatlal M.

    2016-06-01

    Full Text Available Clerodendrum phlomidis L. f. is an important medicinal plant of the Lamiaceae family, particularly its roots, which are used for various therapeutic purposes in a pulverized form. The objective of this study was to develop a standard protocol for axillary shoot proliferation and rooting of C. phlomidis for its propagation and conservation. Nodal explants were inoculated on Murashige and Skoog (MS medium that was supplemented with one of six cytokinins: 6-benzyladenine, kinetin, thidiazuron, N6-(2-isopentenyl adenine (2iP, trans-zeatin (Zea and meta-topolin. Callus induction, which was prolific at all concentrations, formed at the base of nodal explants and hindered shoot multiplication and elongation. To avoid or reduce callus formation with the objective of increasing shoot formation, the same six cytokinins were combined with 4 μM 2,3,5-tri-iodobenzoic acid (TIBA alone or in combination with 270 μM adenine sulphate (AdS. Nodal explants that were cultured on the medium supplemented with 9.12 μM Zea, 4 μM TIBA and 270 μM AdS produced significantly more and longer shoots than on medium without TIBA and AdS. Half-strength MS medium supplemented with 8.05 μM α-naphthaleneacetic acid was the best medium for root formation. Most (75% in vitro rooted plantlets were successfully acclimatized under natural conditions.

  20. LF渣和ρ-Al2 O3结合铝镁质浇注料的相互作用%Interaction of LF slag and ρ-Al2O3 bonded alumina-magnesia castables

    Institute of Scientific and Technical Information of China (English)

    李艳华; 韩兵强; 贺恒星; 刘延山; 李楠

    2014-01-01

    以5~3 mm棕刚玉和3~1、≤1 mm板状刚玉为骨料,以0.074 mm电熔白刚玉、0.088 mm电熔镁砂和0.061 mm尖晶石为细粉,以ρ-Al2O3微粉为结合剂,研究了加入质量分数8%的90尖晶石和加入质量分数7%的97电熔镁砂细粉的铝镁质浇注料常温物理性能、抗LF渣的侵蚀和渗透性能。采用SEM对侵蚀后试样进行了显微结构分析,并利用热力学软件Factsage 6.2对侵蚀过程进行了模拟。结果表明:加入质量分数8%的90尖晶石的铝镁浇注料比加入质量分数7%的97电熔镁砂的铝镁浇注料在各温度处理后的常温强度高,显气孔率低,体积密度大,抗LF渣渗透性能优异。铝镁浇注料中引入较多MgO在高温下大量形成原位尖晶石,易造成较大的体积膨胀,对抗渣渗透性能不利,但有利于吸收渣中FeO、MnO,提高渣的黏度,对抗渣侵蚀性能有利。试验结果与热力学软件模拟结果吻合。%Two batches of alumina-magnesia castables were prepared by using brown corundum (5 -3 mm)and tabular corundum (3-1 and ≤1 mm)as aggregates,fused white alumina (0.074 mm),fused magnesia (0.088 mm)and spinel powder (0.061 mm)as matrix,ρ-Al2O3 as binder,and adding 8 mass%90 spinel and 7 mass%97 fused magnesia,respectively.Cold physical properties,corrosion and penetra-tion resistance to LF slag of the two castables were compared.Microstructures of the two kinds of corroded specimens were investigated with SEM and the corrosion process was thermodynamically simulated by software Factsage 6.2.The results show that:compared with the castable with 7 mass%97 fused magne-sia,the alumina-magnesia castable containing 8 mass%90 spinel has a higher cold strength,a higher bulk density,a lower apparent porosity,and a better penetration resistance to LF slag at all temperatures;high MgO content in alumina-magnesia castables results in more in-situ formed spinel during firing,expanding the volume,which is

  1. VARIAÇÃO BIOMÉTRICA DE DIÁSPOROS DE BURITI (Mauritia flexuosa L.f. – ARECACEAE EM VEREDAS EM DIFERENTES ESTÁGIOS DE CONSERVAÇÃO

    Directory of Open Access Journals (Sweden)

    Fabiano dos Santos Matos

    2014-12-01

    Full Text Available As veredas são fitofisionomias do bioma Cerrado, onde, no estrato arbóreo, predomina a palmeira arbórea Mauritia flexuosa L.f. - buriti, espécie considerada recurso-chave para muitos animais, que a utilizam como moradia e fonte alimentar, e também pelo próprio homem, que usa suas folhas e frutos como matéria-prima e alimento. Apesar de ocorrerem em ambientes protegidos pela legislação ambiental, ações antrópicas nas veredas têm afetado as populações do buriti, por meio da diminuição do número de indivíduos ocasionada por desmatamento, assoreamento e drenagem dessas áreas. O objetivo desse trabalho foi avaliar a morfometria de frutos e sementes de buriti oriundos de seis veredas em diferentes estágios de conservação, situadas na Área de Proteção Ambiental do Rio Pandeiros (norte de Minas Gerais. Foram amostrados, no total, 1800 frutos e sementes, que foram mensurados em comprimento, largura, espessura e peso. Para detectar diferenças das medidas morfométricas dos frutos e das sementes entre as áreas de coleta foi feita a análise de variância. Foram observadas variações nas medidas morfométricas de frutos e sementes entre as áreas de estudo, sendo que 87,5% das maiores médias ocorreram em áreas com menores intervenções antrópicas. Esse fato, possivelmente, está relacionado aos impactos ocorridos nas veredas, além das variações genéticas que podem incidir sobre os indivíduos.

  2. Partial replacement of dried Leucaena leucocephala (Lam.) de Wit leaves for noug (Guizotia abyssinica) (L.f.) Cass. seed cake in the diet of highland sheep fed on wheat straw.

    Science.gov (United States)

    Tesfay, Temesgen; Tesfay, Yayneshet

    2013-02-01

    This study investigated the effect of replacing noug (Guizotia abyssinica) (L.f.) Cass. seed cake by dried Leucaena leucocephala (Lam.) de Wit leaves on feed intake, live weight gain, nutrient digestibility, and nitrogen balance of highland sheep in Tigray Region in northern Ethiopia. Twenty intact yearling male highland sheep weighing 16.9 ± 1.62 kg were used in a randomized complete block design and included the following four treatments: T1 (control, wheat straw ad libitum + 200 g noug seed cake (NSC) + 150 g wheat bran (WB)); T2 (wheat straw ad libitum + 170 g NSC + 44.3 g dried L. leucocephala (DLL) + 150 g WB); T3 (wheat straw ad libitum + 140 g NSC + 87.3 g DLL + 150 g WB); and T4 (wheat straw ad libitum + 110 g NSC + 130.2 g DLL + 150 g WB). Sheep fed on T4 diet consumed higher total dry matter (658 g/head/day) and recorded the highest average daily weight gain (59 g/head/day). Sheep fed on T4 diet had higher dry matter (61 %), organic matter (63 %), and crude protein (75 %) digestibility values than the other treatments. Sheep fed on T3 diet demonstrated higher feed conversion ratio (11.93) than sheep kept on the other treatments. All sheep exhibited positive nitrogen balance, with the highest nitrogen retention being measured in T4 (12 g/head/day). It is concluded that partially replacing NSC by DLL can improve total dry matter intake, digestibility of nutrients, and body weight gain in highland sheep fed on wheat straw as the basal diet.

  3. Estudio de las propiedades físicas y mecánicas, trabajabilidad y secado de la teca (Tectona grandis L.f de Puerto libertador (Córdoba.

    Directory of Open Access Journals (Sweden)

    Betancur Salgado Carlos A.

    2000-06-01

    Full Text Available La presente investigación se realizó en el Laboratorio de Productos Forestales " Héctor Anaya López" de la Universidad Nacional de Colombia, sede Medellín. Dado el interés de la Empresa Reforestadora del Caribe, por conocer las propiedades físicas y mecánicas, trabajabilidad y secado de la madera de teca (Tectona grandis L.f.. ; de cuya especie la Empresa estableció en Noviembre de 1987, una plantación en el municipio de Puerto libertador (Córdoba. Las propiedades físicas que se evaluaron fueron; peso especifico (verde, seco al aire, básico, anhidro y ajustado al 12 % de contenido de humedad, contracción y contenido de humedad. En lo relacionado a las propiedades mecánicas, se realizaron ensayos de flexión estática, compresión paralela a las fibras, compresión perpendicular al las fibras, cizallamiento, dureza, extracción de clavos e impacto; los ensayos se llevaron a cabo tanto en estado verde (CH>30% como en estado seco al aire (CH<15%. La madera presentó peso especifico anhidro (0.57 g/ cm3 , ubicándose dentro del grupo de maderas medianamente pesadas, las propiedades mecánicas de la especie se colocaron en el rango de algo medianas a medianas. Al comparar estos resultados con los estudios reportados para la especie, no se observo diferencia alguna. Los ensayos de trabajabilidad se realizaron en estado seco al aire (CH<15%, evaluando la respuesta de la madera al cepillado, moldurado y taladrado. Los defectos que se presentaron en cepillado fueron grano arrancado, grano velloso y en relieve, siendo de mayor importancia el último, debido a que los demás, se pueden eliminar fácilmente mediante lijado.

  4. Suitability of aquatic biomass from Lake Toba (North Sumatra, Indonesia) for energy generation by combustion process

    Science.gov (United States)

    Brunerová, A.; Roubík, H.; Herák, D.

    2017-09-01

    Several aquatic plant species were identified as aquatic pollution of Lake Toba, North Sumatra (Indonesia); specifically, water hyacinth Eichhornia crassipes and aquatic weeds Hydrilla verticillata and Myriophyllum spicatum due to their high biomass yield which causes impenetrable mats at the bottom and surface of the lake. That complicates other vegetation growth and utilization of water areas for fishing or recreation. In attempt to clean the lake and prevent plants expansion, great amount of plants populations are removed from water but subsequent efficient utilization of such aquatic biomass is not ensured. Present research investigated energy potential of aquatic biomass originated from mentioned aquatic plants from Lake Toba and its possible utilization for energy production by direct combustion. Performed chemical analysis contained from determination of moisture, ash and volatile matter contents and calorific values. Evaluation of results proved highest suitability and energy potential of Eichhornia crassipes with gross calorific value (GCV) 16.31 MJ·kg–1, followed by Hydrilla verticillata with GCV 15.24 MJ·kg–1. Samples of Myriophyllum spicatum exhibited unsatisfactory results due to its low GCV (11.27 MJ·kg–1) in combination with high ash content (36.99%) which indicates complications during combustion, thus, low energy production efficiency and overall unsuitability for combustion purposes.

  5. Influencia de la lateralidad dominante de la jugadora en puesto específico de base en el juego de ataque en Liga Femenina 2 [Laterality dominance of female point guard influences in the offensive patterns in LF2

    Directory of Open Access Journals (Sweden)

    Javier García Rubio

    2010-03-01

    Full Text Available Resumen La lateralidad de la jugadora con el rol de base puede ser sólo una característica individual, o bien un factor influyente en el juego de su equipo, y por tanto, una herramienta a considerar por  parte del equipo rival. El trabajo de investigación se centra en la necesidad de reflejar si la lateralidad de una jugadora con rol de base en baloncesto, al iniciar la acción de juego (bote o pase influye de manera determinante en la toma de decisiones. El contexto de trabajo utilizado es la segunda categoría de baloncesto femenino nacional, Liga Femenina 2. En la observación han participado varios equipos de dicha categoría, con total de 10 partidos analizados y un total de 170 situaciones de juego analizadas (N=170. Para establecer las relaciones entre las variables se utilizó el Coeficiente de Contingencia. Los resultados muestran una relación estadísticamente significativa de la lateralidad de la base y el lado del campo en el que se empieza a jugar (c2(1, N=170 = 4.789, pPalabras clave: Baloncesto, Lateralidad, Base, Análisis de juego.   AbstractLaterality of the point-guard can be an individual characteristic, or in the other hand, an important factor in the offensive patterns of the team. In this way, it will be a useful information for the other team.  This research has focused in the laterality of the point guard, and it influences in the decision making. The League studied was the second female Spanish basketball league, LF2. 10 matches were observed and 170 game situations analyzed (N=170. Crosstabs Command and the Contingency Coefficient were used to establish the relationship between variables. The results shows a significant relationship between the laterality of the point guard and the side of the field where the attack begin (c2(1, N=170 = 4.789, pKey words:  Basketball, laterality, point guard, game analysis.

  6. Influencia de la lateralidad dominante de la jugadora en puesto específico de base en el juego de ataque en Liga Femenina 2 [Laterality dominance of female point guard influences in the offensive patterns in LF2

    Directory of Open Access Journals (Sweden)

    Alejandra Manzano Rivera

    2010-03-01

    Full Text Available Resumen La lateralidad de la jugadora con el rol de base puede ser sólo una característica individual, o bien un factor influyente en el juego de su equipo, y por tanto, una herramienta a considerar por  parte del equipo rival. El trabajo de investigación se centra en la necesidad de reflejar si la lateralidad de una jugadora con rol de base en baloncesto, al iniciar la acción de juego (bote o pase influye de manera determinante en la toma de decisiones. El contexto de trabajo utilizado es la segunda categoría de baloncesto femenino nacional, Liga Femenina 2. En la observación han participado varios equipos de dicha categoría, con total de 10 partidos analizados y un total de 170 situaciones de juego analizadas (N=170. Para establecer las relaciones entre las variables se utilizó el Coeficiente de Contingencia. Los resultados muestran una relación estadísticamente significativa de la lateralidad de la base y el lado del campo en el que se empieza a jugar (c2(1, N=170 = 4.789, pPalabras clave: Baloncesto, Lateralidad, Base, Análisis de juego.   AbstractLaterality of the point-guard can be an individual characteristic, or in the other hand, an important factor in the offensive patterns of the team. In this way, it will be a useful information for the other team.  This research has focused in the laterality of the point guard, and it influences in the decision making. The League studied was the second female Spanish basketball league, LF2. 10 matches were observed and 170 game situations analyzed (N=170. Crosstabs Command and the Contingency Coefficient were used to establish the relationship between variables. The results shows a significant relationship between the laterality of the point guard and the side of the field where the attack begin (c2(1, N=170 = 4.789, pKey words:  Basketball, laterality, point guard, game analysis.

  7. 基于ITS与 trn L-F序列探讨槭树科的系统发育%Phylogeny of Aceraceae Based on ITS and trn L-F Data Sets

    Institute of Scientific and Technical Information of China (English)

    田欣; 郭振华; 李德铢

    2002-01-01

    The nuclear encoded internal transcribed spacer (ITS) region and the plastid encoded trn L-F region were sequenced for 41 species of the Aceraceae,representing both genera Acer and Dipteronia,to reconstruct phylogeny of this family,especially within Acer.The analyses were performed in separate and combined sequence data sets,with the Sapindaceae and Hippocastanaceae being selected as outgroups.It was indicated that the Aceraceae was monophyletic and D.sinensis was basal to the rest of the family but the two genera of it might be not monophyletic because Dipteronia dyerana was nested within Acer.The result inferred from the combined data showed greater resolution within Acer than that from the two separate data sets.The monophyly of most sections in Xu's system (1996) were supported with high bootstrap values,and some relationships between (or among) sections were also inferred,such as sect.Palmata and sect.Microcarpa ; sect.Platanoidea ,sect.Lithocarpa and sect.Macrophylla ; sect.Integrifolia ,sect.Trifoliata and sect.Pentaphylla ; and sect.Acer ,sect.Goniocarpa and sect.Saccharina (sensu Ogata).However,the sectional status and circumscriptions of some of the above-mentioned sections should be further adjusted.It seemed that the Xu's delimitations of sect.Rubra and sect.Saccharodendran should be revaluated.%报道了槭树科41种(其中槭属39种)植物的 trn L-F和ITS序列(其中部分种的ITS序列为重新测定),以期通过分子手段对槭树科内部尤其是复杂的槭属的系统发育关系进行重建.以无患子科和七叶树科为外类群,基于对57个种单独的ITS序列(包括从GenBank下载的16种的序列)、41种 trn L-F序列及41种两者序列的联合数据,分别采用最大简约法(Maximum Parsimony Method)和邻接法(Neighbor-Joining Method)对槭树科的系统发育进行了分析.结果显示,整个槭树科为一单系类群;金钱槭位于槭树科的基部;但由于云南金钱槭( Dipteronia dyerana )聚在了槭属

  8. 葵花籽油热氧化过程的1H-NMR指纹图谱与LF-NMR弛豫特性研究%1H Nuclear Magnetic Resonance (NMR) Fingerprints and Low-Field-NMR Relaxation Properties of Sunflower Oil during Thermal Oxidation

    Institute of Scientific and Technical Information of China (English)

    卢海燕; 王欣; 赵婷婷; 刘宝林

    2014-01-01

    论文应用1H-NMR研究了葵花籽油65℃贮藏过程中氧化产物(共轭烯、醛类、氧化物、芳香烃、游离酸相对含量)的变化规律,并与低场核磁共振(LF-NMR)弛豫特性中的S21的变化进行了相关性分析.1H-NMR结果表明,在65℃贮藏过程中,油样的初级氧化产物随贮藏时间的延长呈先增加后减小的趋势,与氢过氧化物相比,氢过氧化物-共轭二烯优先降解;次级氧化产物及水解产物如醛、环氧化物、游离脂肪酸等随贮藏时间呈良好指数关系(R2>0.96),芳香烃则随贮藏时间呈先增加后减小的趋势.而LF-NMR检测所得到的油样的S21随贮藏时间延长呈良好的指数增加趋势(R2=0.99).相关性分析表明,氧化物、游离脂肪酸与S21的相关性最高,模型验证合理可靠.该结果可为研究S21特征峰的表征物质、提高LF-NMR油脂品质判别率提供研究参考及有益思路.

  9. Habitat selection by three littoral zone fishes: effects of predation pressure, plant density and macrophyte type

    Science.gov (United States)

    Chick, J.H.; McIvor, C.C.

    1997-01-01

    We conducted laboratory experiments which demonstrated that three littoral zone fishes differentially selected among three macrophytes when seeking refuge from predation. In the presence of a predator (a juvenile Micropterus salmoides), mosquitofish (Gambusia holbrooki), sailfin mollies (Poecilia latipinna). and dollar sunfish (Lepomis marginatus) displayed ferential use of four tank areas containing patches of either Hydrilla verticillata, Potamogeton illinoensis, Panicum hemitomon, or no plants. Patterns habitat selection, and the consistency of these patterns among replicates, differed among the three fishes and among three plant-density treatments - natural (each macrophyte presented at its mean field density), equal (all three macrophytes at the same density), and control (no plants). Selection for H. verticillata by mosquitofish was significant for both the equal and natural treatments, and thus was not caused by differences in plant density alone. Sailfin mollies displayed significant selection for H. verticillata only in the natural plant-density treatments. Dollar sunfish showed less consistent habitat selection than either mosquitofish or sailfin mollies. Significant habitat selection was not found in the absence of a predator, and there was no evidence for lection among the tank areas in control treatments. Patterns of habitat selection by the three fishes in our laboratory study corresponded to observed habitat use in Lake Okeechobee.

  10. Effects of competitive interactions of different life forms submersed plants on biomass allocation in shallow lakes

    Institute of Scientific and Technical Information of China (English)

    ZHANG Xiu-feng

    2010-01-01

    Plant competition has been recognized as one of the most important factors influencing the structure and function of lake ecosystems.Competition from plants of dissimilar growth form may have profound effects on shallow lakes.An experiment was conducted to investigate the effects of competitive interactions of submersed plants with dissimilar growth forms on the biomass allocations.Hydrilla verticillata and Vallisneria natans were selected and were planted in a single-species monoculture and a mixed-species pattern.Results showed that the growth of Ⅴ.natans was significantly affected by the H.verticillata and caused a sharp reduction of biomass,but the root:shoot ratio of Ⅴ.natans was not affected significantly and there was a minimal increase in mixture: while for H.verticillata,the biomass and the root:shoot ratio were not significantly changed by the competitive interactions of Ⅴ.natans,there was minimal increase qf biomass and minimal decrease of the root:shoot ratio.These results may indicate that the phant which candevelop a dense mat or canopy at the water surface would be a stronger competitor relative to the plant that dependsmore on light availability near the sediment.

  11. R. Bohlin, De Osynliga. Det Europas fattiga arbetarklass; M. Linton, De hatade. Om radikalhögerns måltavlor; B. Elmbrant, Europas stålbad. Krisen som slukar välfärden och skakar euron (All titles by Atlas, Stockholm, 2012

    Directory of Open Access Journals (Sweden)

    Monica Quirico

    2014-03-01

    Full Text Available A review of the following volumes: Bohlin, Rebecka (2012, De Osynliga. Det Europas fattiga arbetarklass (The invisibles. Europe’s poor working class, Stockholm: Atlas; Linton, Magnus (2012, De hatade. Om radikalhögerns måltavlor (The hated. About the targets of the radical right, Stockholm: Atlas; Elmbrant, Björn (2012, Europas stålbad. Krisen som slukar välfärden och skakar euron (Europe’s baptism of fire. The crisis swallowing Welfare State and shaking euro, Stockholm: Atlas.

  12. BLG gene knockout and hLF gene knock-in at BLG locus in goat by TALENs%转录激活因子样效应物核酸酶介导的山羊β-乳球蛋白基因敲除和人乳铁蛋白基因定点整合

    Institute of Scientific and Technical Information of China (English)

    宋绍征; 朱孟敏; 袁玉国; 荣耀; 徐晟; 陈思; 梅珺琰; 成勇

    2016-01-01

    为了敲除山羊乳中致敏源β-乳球蛋白(BLG)基因,同时在BLG基因座定点整合人乳铁蛋白(hLF)基因.首先针对山羊BLG第3外显子识别位点设计了1对特异性TALEN-3-L/R质粒对;同时,构建了含有1个HSV-TK负筛选基因的hLF基因打靶载体BLC14-TK. TALENs质粒对转染山羊胎儿成纤维细胞,2 μg/mL嘌呤霉素筛选3d,PCR扩增产物测序来验证其切割DNA活性.打靶载体BLC14-TK与TALEN-3-L/R质粒对共转染山羊胎儿成纤维细胞,经700 μg/mL G418和2 μg/mL GCV共筛选药物抗性细胞株;通过整合检测和同源重组检测来筛选hLF基因打靶细胞株;BLG-/hLF+打靶细胞株作为供核细胞进行山羊体细胞核移植.结果为:TALEN-3-L/R致突变率为25%-30%;获得BLG-/hLF+打靶细胞6株;共制作重构胚胎335枚,移植受体山羊23只,B超检测到30-35 d的妊娠受体9只(妊娠率39.1%),其中1只50日龄克隆胎儿验证为BLG-/hLF+基因型.以上结果表明获得BLG基因座定点整合hLF基因的基因打靶山羊是可行的,为培育羊乳中含低致敏原和害含hLF的山羊新品系奠定了基础.

  13. Field and laboratory evaluation of the influence of copper-diquat on apple snails in southern Florida

    Science.gov (United States)

    Winger, P.V.; Imlay, M.J.; McMillan, W.E.; Martin, T.W.; Takekawa, Jean E.; Johnson, W.W.

    1984-01-01

    The recent decline of apple snail (Pomacea paludosa) populations in canals surrounding Loxahatchee National Wildlife Refuge in southern Florida coincided with the use of copper-diquat for the control of the aquatic weed hydrilla (Hydrilla verticillata). Field and laboratory studies were designed to assess the effects of copper-diquat on apple snails, which are the primary food of the endangered snail kite Rostrhamus sociabilis (formerly known as the Everglade kite). Acute toxicities (96-h LC50 values) of Cutrine-Plus and Komeen (chelated formulations of copper) to immature apple snails were 22 and 24 μg/L, respectively. Diquat was toxic at a concentration of 1,800 μg/L and did not increase the toxicity of copper when the chemicals were used in combination. Evaluation of field samples indicated that copper concentrations were higher in detritus than in water, plants and mud, and that there was a gradient of copper concentration from the canal to the interior, the highest residues being in samples from the canal. Copper associated with detritus (up to 150 μg/g) had no effect on growth or survival of apple snails in field cage and tank studies. Also, field applications of copper-diquat to hydrilla had no effect on survival of caged adult and immature snails. Copper from field applications was rapidly taken out of solution by plants and organic material in the water and subsequently incorporated into the bottom detritus. Although the effects of repeated applications of copper-diquat and high body burdens of copper (accumulated during exposure to herbicidal treatment) on survival and reproduction of apple snails are not known, the information available indicates that treatment of hydrilla with copper-diquat was probably not responsible for the decline in the apple snail population. Application at recommended rates should pose no threat to these snails in the organically rich waters of southern Florida.

  14. Ecology of rare water plant communities in lakes of north-eastern Poland

    Directory of Open Access Journals (Sweden)

    Ewa Jabłońska

    2012-03-01

    Full Text Available Habitat studies were conducted on three rare plant communities dominated by Nuphar pumila, Nymphaea candida and Hydrilla verticillata in lakes of north-eastern Poland. The comparison of habitat properties of these three types of phytocoenoses with those of Nuphar lutea common in the area under study was also performed. It was demonstrated that the plant communities studied were ecologically distinct. The habitats of the phytocoenoses of N. pumila differed most significantly from those of the other phytocoenoses. They often inhabited softer waters poor in Mg2+, dissolved SiO2, but rich in total Fe, PO43−, NO3−, and were associated with acidic substrates containing lower levels of Ca2+ and Na+, but greater amounts of total Fe and NO3−. The differences in the habitats of H. verticillata and N. candida phytocoenoses were most pronounced in the case of four properties of water: Na+, K+, Cl−, and Mg+. Their values were lower in waters of the H. verticillata phytocoenoses. The habitats of all the three types of rare phytocoenoses differed considerably from those of N. lutea. The most significant differences were found between the N. lutea and N. pumila phytocoenoses and the smallest differences were between the patches of N. lutea and N. candida. The properties of water were more important in differentiating the habitats of the phytocoenoses studied than the substrate properties. Due to alkalization and increase in water hardness in the lakes studied the stands of N. pumila are among the most threatened. The patches of N. candida and H. verticillata, which occur in waters with a wider range of hardness and tolerating a slight increase in trophy, can still continue to persist in the lakes for a long time.

  15. Do Amplitudes of Water Level Fluctuations Affect the Growth and Community Structure of Submerged Macrophytes?

    Science.gov (United States)

    Wang, Mo-Zhu; Liu, Zheng-Yuan; Luo, Fang-Li; Lei, Guang-Chun; Li, Hong-Li

    2016-01-01

    Submerged macrophytes are subjected to potential mechanical stresses associated with fluctuating water levels in natural conditions. However, few experimental studies have been conducted to further understand the effects of water level fluctuating amplitude on submerged macrophyte species and their assemblages or communities. We designed a controlled experiment to investigate the responses of three submerged macrophyte species (Hydrilla verticillata, Ceratophyllum demersum and Elodea nuttallii) and their combinations in communities to three amplitudes (static, ± 30 cm, ± 60 cm) of water level fluctuations. Results showed that water level fluctuating amplitude had little effects on the community performance and the three tested species responded differently. H. verticillata exhibited more growth in static water and it was negatively affected by either of the water level fluctuations amplitude, however, growth parameters of H. verticillata in two fluctuating water level treatments (i.e., ± 30 cm, ± 60 cm) were not significantly different. On the other hand, the growth of C. demersum was not significantly correlated with different amplitude treatments. However, it became more abundant when water levels fluctuated. E. nuttallii was inhibited by the two fluctuating water level treatments, and was less in growth parameters compared to the other species especially in water level fluctuating conditions. The inherent differences in the adaptive capabilities of the tested species indicate that C. demersum or other species with similar responses may be dominant species to restore submerged macrophyte communities with great fluctuating water levels. Otherwise, H. verticillata, E. nuttallii or other species with similar responses could be considered for constructing the community in static water conditions.

  16. Mechanism of freeze-thaw treatment influencing quality of puffing product for sweet potato using low-field nuclear magnetic resonance%利用LF-NMR探讨冻融处理影响甘薯膨化产品品质的机理

    Institute of Scientific and Technical Information of China (English)

    郭婷; 何新益; 邓放明; 陈益能

    2013-01-01

      为探究冻融处理对甘薯变温压差膨化干燥产品品质影响的机理,应用低场核磁共振技术(low-field nuclear magnetic resonance,LF-NMR)研究了冻融后甘薯中水分存在形式和各组分含量,比较了冻融次数对甘薯膨化干燥产品硬度、色泽和多孔性等品质指标的影响。结果表明:冻融后甘薯LF-NMR自旋-自旋弛豫时间T2谱中出现4个水分峰,其横向弛豫时间分别为T21(0.25~0.55 ms)、T22(1~2.5 ms)、T23(5~12 ms)、T24(40~200 ms)。随着冻融次数增加,自由水含量(mT24)先增加后减少,结合最紧密的水含量(mT21)先减少后增加;干燥产品多孔性和复水性逐渐增大,ΔE逐渐变小,L*和硬度先减小后有稍许增大。相关性分析表明,多孔性与T24、mT23呈现显著正相关(P<0.05),相关系数分别为0.995、0.989;mT22与ΔE的相关系数为0.984。该研究为阐明冻融处理对果蔬变温压差膨化干燥品质变化机理分析提供了理论依据。%The water content is generally 70%to 90%in fresh fruits and vegetable, which usually includes free water, immobilized water, and closely combined water. The water content and its distribution in the raw materials of fruit and vegetable may affect the drying process, physicochemical, and sensory characteristics of dry products dried by different drying technologies. There are a lot of drying methods reported, such as natural sun drying, vacuum drying, heat pump drying, forced-air drying, microwave drying, convective drying, freeze-drying, and explosion puffing drying. Nuclear magnetic resonance (NMR) is a physical phenomenon generated from the interaction between alternating magnetic field and substance. A pulse NMR system has been widely used in the investigation of bound water in a biological system in recent years. Among them, low-field nuclear magnetic resonance (LF-NMR) is a rapid, non-destructive analysis technique, in

  17. [Bioremediation efficiency of applying Daphnia magna and submerged plants: a case study in Dishui Lake of Shanghai, China].

    Science.gov (United States)

    Huo, Yuan-Zi; He, Wen-Hui; Luo, Kun; Wang, Yang-Yang; Zhang, Yin-Jiang; Tian, Qian-Tao; He, Pei-Min

    2010-02-01

    From April 2007 to January 2008, a bioremediation experiment was conducted in a diversion channel of D-port pilot area of Dishui Lake (the channel length is 950 m, and its water volume is 10000 m3). Daphnia magna was first introduced to filter the high biomass of phytoplankton and other particulate organic matter, and then, five submerged plant species Elodea canadensis, Vallisneria spiralis, Hydrilla verticillata, Potamogeton lucens, and Potamogeton crispus were transplanted. Water samples were collected monthly to monitor the water quality and to investigate the bioremediation efficiency. Ten months monitoring data showed that in the remediation area, the water body's total nitrogen (TN), ammonium nitrogen (NH4(+)-N), nitrate nitrogen (NO3(-)-N), nitrite nitrogen (NO2(-)-N), total phosphorus (TP), and reactive phosphate (PO4(3-)-P) concentrations and chemical oxygen demand (COD) were significantly lower (P study demonstrated the effectiveness of introducing D. magna and transplanting submerged plants in improving the water quality of Dishui Lake.

  18. Non-contact intracellular binding of chloroplasts in vivo

    Science.gov (United States)

    Li, Yuchao; Xin, Hongbao; Liu, Xiaoshuai; Li, Baojun

    2015-06-01

    Non-contact intracellular binding and controllable manipulation of chloroplasts in vivo was demonstrated using an optical fiber probe. Launching a 980-nm laser beam into a fiber, which was placed about 3 μm above the surface of a living plant (Hydrilla verticillata) leaf, enabled stable binding of different numbers of chloroplasts, as well as their arrangement into one-dimensional chains and two-dimensional arrays inside the leaf without damaging the chloroplasts. Additionally, the formed chloroplast chains were controllably transported inside the living cells. The optical force exerted on the chloroplasts was calculated to explain the experimental results. This method provides a flexible method for studying intracellular organelle interaction with highly organized organelle-organelle contact in vivo in a non-contact manner.

  19. Bioenergy potential of eight common aquatic weeds

    Energy Technology Data Exchange (ETDEWEB)

    Abbasi, S.A.; Nipaney, P.C.; Schaumberg, G.D. (Pondicherry (Central) Univ. (IN). Salim Ali School of Ecology)

    1990-01-01

    Eight common aquatic weeds Salvinia molesta, Hydrilla verticillata, Nymphaea stellata, Azolla pinnata, Ceratopteris sp. Scirpus sp. Cyperus sp, and Utricularia reticulata were digested anaerobically to produce methane. The carbon to nitrogen (C/N) ratio, carbon to phosphorus (C/P) ratio, and the volatile solids (VS) content of the weeds varied widely. No trend between these factors and the methane yield was discernable; the possible reasons are discussed. The energy potential of the weeds per unit area of the weed crop was worked out. Natural stands of salvinia, such as the one employed in the present investigation, would yield energy (methane) of the order of 10{sup 8} Kcal/ha/yr. (author).

  20. Heavy metal induced DNA changes in aquatic macrophytes: Random amplified polymorphic DNA analysis and identification of sequence characterized amplified region marker

    Institute of Scientific and Technical Information of China (English)

    Meetu Gupta; Neera Bhalla Sarin

    2009-01-01

    Plants have been used as good bio-indicators and genetic toxicity of environmental pollution in recent years. In this study, aquatic plants Hydrilla verticillata and Ceratophyllum demersum treated with 10 mol/L Cd, 5 mol/L Hg, and 20 mol/L Cu for 96 h, showed changes in chlorophyll, protein content, and in DNA profiles. The changes in DNA profiles included variation in band intensity, presence or absence of certain bands and even appearance of new bands. Genomic template stability test performed for the qualitative measurement of changes in randomly amplified polymorphic DNA (RAPD) profiles, showed significant effect at the given concentration of metals. Cloning and sequencing of bands suggested that these markers although may not be homologous to any known gene but its conversion as a sequence characterized amplified region (SCAR) marker is useful in detecting the effects of genotoxin agents.

  1. Change of LF-NMR relaxation properties of sunflower seed oil at different temperatures and its correlation with physicochemical indexes%不同温度下葵花籽油低场核磁弛豫特性的变化及与理化指标的关系

    Institute of Scientific and Technical Information of China (English)

    2016-01-01

    借助于低场核磁共振(LF-NMR)技术快速监测葵花籽油理化品质的变化,有利于油脂品质及安全保障.对葵花籽油在105℃和180℃氧化过程中的LF-NMR弛豫特性变化规律进行了研究,并应用逐步回归分析了其与多个理化指标间的相关性.结果表明:在105℃氧化过程中,S21和T22变化规律较为明显,分别呈二项式上升和下降趋势,其他弛豫特性变化规律不明显;相关性分析表明,除酸值和碘值外,过氧化值、共轭二烯、共轭三烯和茴香胺值均与S21和T22呈显著相关(P≤0.05)或极显著相关(P≤0.01),极性化合物与T21 、S21 、S22和T23均呈显著或极显著相关,预测模型经验证后发现,仅过氧化值、共轭二烯和极性化合物含量模型可靠(R2 >0.78);在180℃氧化过程中,S21和S22呈二项式上升,T21呈线性下降,T22和S23呈二项式下降趋势,且S21、T21、T22和S23均与除过氧化值外的其他理化指标呈显著相关或极显著相关,T23变化规律不明显,只有S22与过氧化值相关性显著;除过氧化值外,所有理化指标预测模型均建立准确(R2 >0.89),验证可靠(R2 >0.85).

  2. Association Analysis of Polymorphism in the Bovine Lactoferrin Gene Exon11 with the Susceptibility to Mastitis and Part Milk Trait in Simmental Cattles%西门塔尔牛bLF-exon11基因多态性及其与乳房炎易感性和部分乳性状的相关分析

    Institute of Scientific and Technical Information of China (English)

    王国富; 吴慧光; 孙国权; 李俊雅; 高雪; 王景山; 王玉泉; 高树新

    2012-01-01

    To explore the association of polymorphisms of bovine lactoferrin(bLF) gene with the susceptibility to mastitis and part milk trait,PCR-SSCP and DNA sequencing techniques were used to detect the polymorphisms of exonll of 38 case cattle and 118 control cattle. The results analysis showed that, the extremely significant differences in genotypic frequencies and allelic frequencies of exonll were present in case group and control group(P < 0. 01 ) , AA genotypic frequencies and A allelic frequencies of case group were extremely significant higher than the control group(P <0. 01 ). Moreover, the SCS of AA genotype individuals were significant higher than AB and BB genotype (P <0.05) ,the milk protein of A A genotype individuals were extremely significant higher than AB genotype(P<0.01) ,and significant higher than BB genotype(P <0. 05 ) ,the BB genotype individuals were significant higher than AB genotype (P <0.05) . These findings indicated a prospect, the A allelic of bLF-exonll may have relevant to the susceptibility of mastitis in Simmental cattle.%为了探讨西门塔尔牛乳铁蛋白基因第11外显子多态性,及其与乳房炎和部分乳性状的关系,利用PCR-SSCP和DNA测序技术,对118头健康牛和38头感染乳房炎牛个体,进行了第11外显子的基因多态性分析.结果发现,健康牛和感染乳房炎牛个体的第11外显子基因型和基因频率分布差异均极显著(P<0.01),感染牛中AA基因型和A基因频率均极显著高于健康牛(P<0.01).而且,AA基因型个体SCS显著高于AB和BB型个体(P<0.05),AA型个体的乳蛋白极显著高于AB型(P<0.01)、显著高于BB型(P<0.05),而BB型显著高于AB型(P<0.05).这表明乳铁蛋白基因第11外显子的A基因,可能是一个西门塔尔牛乳房炎的易感基因.

  3. Effect of packaging and storage temperature on water holding capacity of catfish fillets during storage based on low field NMR%基于 LF-NMR 研究包装方式和温度对鲶鱼片保水性的影响

    Institute of Scientific and Technical Information of China (English)

    朱迎春; 马俪珍; 党晓燕; 王洋

    2016-01-01

    The effect of packaging and storage temperature on the water holding capacity (WHC) of catfish fillet (Clarias gariepinus) was investigated using the low-field nuclear magnetic resonance (LF-NMR). The catfish fillets were stored at 4°C or -0.7°C with air-packaging (AP), vacuum-packaging (VP) or modified atmosphere-packaging (MAP, 60% CO2 and 40% N2). The fillets were sampled on the 0, 1, 4, 7, 10, 15, 20, 30 day since storage to analyze the pH value, cooking loss and water loss after centrifugation. The WHC changes of catfish fillets were illustrated by relaxation time, relaxation area and proton density in LF-NMR, and muscle fiber structure changes were visualized by the scanning electronic microscopy. The present study aimed to give a comprehensive judgment on the process of WHC changes of the catfish fillet during the storage at different temperatures and with different packaging styles, and aimed to reveal the underlying mechanism. The results demonstrated that the WHC of the catfish fillet decreased during the storage period. The pH value significantly declined in each treatment group during the first 4 days; later, the pH value of the AP and VP groups stored at both 4 ℃ and -0.7 ℃ rose significantly (P<0.05), while that of MAP groups (4 ℃ and -0.7 ℃) was maintained at low levels of 6.51-6.88. The highest cooking loss occurred in the 4 ℃ VP group which increased from 11.54% (the initial day) to 23.70% (the 4th day). Water loss after centrifugation significantly rose from 15.98% (the initial day) to 31.35% (the 15th day) in the 4 ℃ VP group. Therefore it can be concluded that the VP is not an effective package style to maintain the WHC. The relaxation time showed a downward tendency from 63.67 (the initial day) to 54.82 (4 ℃ AP group, the 10th day) and 57.10 ms (0.7 ℃ AP group, the 15th day), which was because the binding force of water increased, and the degree of freedom and the mobility of water decreased. The relaxation area increased from

  4. The distribution of submersed aquatic vegetation and water lettuce in the fresh and oligohaline tidal Potomac River, 2007

    Science.gov (United States)

    Campbell, Sarah Hunter; Rybicki, Nancy B.; Schenk, Edward R.

    2015-01-01

    Surveys documenting the composition of species of submersed aquatic vegetation (SAV) have been conducted in the Potomac River for decades. These surveys can help managers assess the proportion of native and exotic plants in the river or can be used to determine relationships between native and exotic plants, environmental conditions, and wildlife. SAV coverage increased from 2005 to 2007 throughout the fresh and oligohaline study area. The 2007 survey documented here determined that eleven species of SAV were present. The abundance of the exotic species Hydrilla verticillata (hydrilla) was relatively low, and species diversity was relatively high compared to previous years. The survey also revealed a new population of the invasive, floating aquatic plant Pistia stratiotes (water lettuce). In 2007, water lettuce, the latest exotic aquatic plant to be found in the fresh to oligohaline portion of the Potomac River, was most abundant in Mattawoman Creek, Charles County, Maryland. However, it was not observed in the fresh to oligohaline portion of the Potomac River in the summer of 2008. An understanding of the distribution of SAV species and factors governing the abundance of native and invasive aquatic species is enhanced by long-term surveys.

  5. Bacterial community variation and microbial mechanism of triclosan (TCS) removal by constructed wetlands with different types of plants.

    Science.gov (United States)

    Zhao, Congcong; Xie, HuiJun; Xu, Jingtao; Xu, Xiaoli; Zhang, Jian; Hu, Zhen; Liu, Cui; Liang, Shuang; Wang, Qian; Wang, Jingmin

    2015-02-01

    Triclosan (TCS) is a broad-spectrum synthetic antimicrobial agent that is toxic to microbes and other aquatic organisms. Constructed wetlands (CWs) are now popular in TCS removal. However, knowledge on the effects of TCS on the bacterial community and microbial removal mechanism in CWs is lacking. The effects of TCS (60 μg L(-1)) on bacterial communities in batch-loaded CWs with emergent (Typha angustifolia), submerged (Hydrilla verticillata), and floating plant (Salvinia natans) were analyzed by 454 pyrosequencing technology. After six periods of experiment, the TCS removal efficiencies were over 90% in CWs, and negative effects of TCS on bacterial community richness and diversity were observed. Moreover, plant species effect existed. Bacterial strains that contributed to TCS biodegradation in CWs were successfully identified. In TCS-treated T. angustifolia and H. verticillata CWs, beta-Proteobacteria increased by 16.63% and 18.20%, respectively. In TCS-treated S. natans CWs, delta- and gamma-Proteobacteria and Sphingobacteria increased by 9.36%, 19.49%, and 31.37%, respectively, and could relate to TCS biodegradation. TCS affected the development of certain bacteria, and eventually, the bacterial community structures in CWs. This research provided ecologically relevant information on bacterial community and microbial removal mechanism in CWs under TCS treatment. Copyright © 2014 Elsevier B.V. All rights reserved.

  6. Biosorption and growth inhibition of wetland plants in water contaminated with a mixture of arsenic and heavy metals

    Energy Technology Data Exchange (ETDEWEB)

    Park, Soyoung [Department of Ocean Engineering, Pukyong National University, Busan (Korea, Republic of); Kang, Daeseok; Kim, Youngyun; Lee, Suk Mo; Chung, Yonghyun; Sung, Kijune [Department of Ecological Engineering, Pukyong National University, Busan (Korea, Republic of)

    2011-02-15

    The potential of wetland plants as an onsite biosorbent and a biomonitor for combined pollution of arsenic and four heavy metals from non-point sources was investigated in this study. Ceratophyllum demersum, Hydrilla verticillata, Hydrocharis dubia, and Salvinia natans were exposed to a water containing mixture of As, Cr, Cu, Pb, and Zn. Growth inhibition and biosorption potential of the wetland plants in artificially contaminated conditions were studied. These contaminants significantly reduced the growth of the plants. The tested wetland plants accumulated appreciable amounts of the contaminants in the following order: Pb>Cr>Cu>Zn>As. H. verticillata showed distinct visual change and a high biosorption factor (BSF) rank for As and heavy metals among the plants used in the study. As an unspecific collector of contaminants, it might be useful as a biomonitor and biosorbent in the As and heavy metal-contaminated aquatic system. (Copyright copyright 2011 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  7. Evaluation of the Environmental DNA Method for Estimating Distribution and Biomass of Submerged Aquatic Plants.

    Science.gov (United States)

    Matsuhashi, Saeko; Doi, Hideyuki; Fujiwara, Ayaka; Watanabe, Sonoko; Minamoto, Toshifumi

    2016-01-01

    The environmental DNA (eDNA) method has increasingly been recognized as a powerful tool for monitoring aquatic animal species; however, its application for monitoring aquatic plants is limited. To evaluate eDNA analysis for estimating the distribution of aquatic plants, we compared its estimated distributions with eDNA analysis, visual observation, and past distribution records for the submerged species Hydrilla verticillata. Moreover, we conducted aquarium experiments using H. verticillata and Egeria densa and analyzed the relationships between eDNA concentrations and plant biomass to investigate the potential for biomass estimation. The occurrences estimated by eDNA analysis closely corresponded to past distribution records, and eDNA detections were more frequent than visual observations, indicating that the method is potentially more sensitive. The results of the aquarium experiments showed a positive relationship between plant biomass and eDNA concentration; however, the relationship was not always significant. The eDNA concentration peaked within three days of the start of the experiment in most cases, suggesting that plants do not release constant amounts of DNA. These results showed that eDNA analysis can be used for distribution surveys, and has the potential to estimate the biomass of aquatic plants.

  8. Accumulation of microcystin congeners in different aquatic plants and crops--a case study from lake Amatitlán, Guatemala.

    Science.gov (United States)

    Romero-Oliva, Claudia Suseth; Contardo-Jara, Valeska; Block, Tobias; Pflugmacher, Stephan

    2014-04-01

    Microcystins (MCs) fate in natural environments can lead to its transfer into aquatic organisms, e.g. aquatic plants. Moreover, lakes in several countries sustain agriculture activities posing a serious health threat for the public. The case of Lake Amatitlán in Guatemala, was addressed to better understand MCs accumulation of four aquatic plants (Polygonum portoricensis, Eichhornia crassipes, Typha sp. and Hydrilla verticillata) coexisting with Microcystis aeruginosa blooms. These findings were further corroborated with an uptake/accumulation laboratory study. Finally crop products (Solanum lycopersicum and Capsicum annuum) irrigated with lake water were also evaluated for MCs. The obtained results suggest that Lake Amatitlán is highly contaminated with MCs (intra- and extracellular 1931 and 90 µg/L, respectively). In fruits of S. lycopersicum and C. annuum a concentration of 1.16 and 1.03 µg/kg dry weight (DW), respectively could be detected. All four aquatic plants showed a high MCs uptake capacity based on obtained bioconcentration factors (BCF) 165, 18, 16 and 11, respectively. These results were further corroborated in a laboratory study with 30 percent of total MCs taken up by H. verticillata within just 7 days. Additionally it was evidenced that all plants accumulated more MC-LR than other MCs congeners. Monitoring of crop products irrigated with lake water needs further consideration.

  9. Vegetative Propagule Pressure and Water Depth Affect Biomass and Evenness of Submerged Macrophyte Communities.

    Science.gov (United States)

    Li, Hong-Li; Wang, Yong-Yang; Zhang, Qian; Wang, Pu; Zhang, Ming-Xiang; Yu, Fei-Hai

    2015-01-01

    Vegetative propagule pressure may affect the establishment and structure of aquatic plant communities that are commonly dominated by plants capable of clonal growth. We experimentally constructed aquatic communities consisting of four submerged macrophytes (Hydrilla verticillata, Ceratophyllum demersum, Elodea nuttallii and Myriophyllum spicatum) with three levels of vegetative propagule pressure (4, 8 and 16 shoot fragments for communities in each pot) and two levels of water depth (30 cm and 70 cm). Increasing vegetative propagule pressure and decreasing water level significantly increased the growth of the submerged macrophyte communities, suggesting that propagule pressure and water depth should be considered when utilizing vegetative propagules to re-establish submerged macrophyte communities in degraded aquatic ecosystems. However, increasing vegetative propagule pressure and decreasing water level significantly decreased evenness of the submerged macrophyte communities because they markedly increased the dominance of H. verticillata and E. nuttallii, but had little impact on that of C. demersum and M. spicatum. Thus, effects of vegetative propagule pressure and water depth are species-specific and increasing vegetative propagule pressure under lower water level can facilitate the establishment success of submerged macrophyte communities.

  10. Epiphytic bacterial communities on two common submerged macrophytes in Taihu Lake: diversity and host-specificity

    Institute of Scientific and Technical Information of China (English)

    HE Dan; REN Lijuan; WU Qinglong

    2012-01-01

    Leaves of terrestrial and aquatic plants are home to a wide diversity of bacterial species.However,the diversity and variability of epiphytic bacteria on their submerged plant hosts remains poorly understood.We investigated the diversity and composition of epiphytic bacteria from two common submerged macrophytes:Vallisneria natans and Hydrilla verticillata in Taihu Lake,Jiangsu,China,using methods of terminal restriction fragment length polymorphisms (T-RFLP) and clone library analyses targeted at bacterial 16S rRNA genes.The results show that:(1) the libraries of the two waterweeds contain wide phylogenetic distribution of bacteria,and that the sequences of the two libraries can be separated into 93 OTUs (at 97% similar value); (2) Betaproteobacteria,including Burkholderiales,was the most abundant bacterial group on both plants.Cyanobacteria and Gammaproteobacteria were the second largest groups on V.natans and H.verticillata,respectively.Both clone libraries included some sequences related to those of methanotrophs and nitrogen-fixing bacteria; (3) Cluster analysis of the T-RFLP profiles showed two distinct clusters corresponding to the two plant populations.Both ANOSIM of the T-RFLPdata and Libshuff analysis of the two clone libraries indicated a significant difference in epiphytic bacterial communities between the two plants.Therefore,the epiphytic bacterial communities on submerged macrophytes appear to be diverse and host-specific,which may aid in understanding the ecological functions of submerged macrophytes in general.

  11. VLF/LF High-Voltage Design and Testing

    Science.gov (United States)

    2003-09-01

    This equation is plotted in Figure 3-3 and accurately fits the measured vapor pressure data (Sears & Zemansky , 1955). A psychrometric chart is a...determined as follows. Relative humidity (RH) is defined by the following equation (Sears & Zemansky , 1955). %100×= V w P P RH (3-6) where Pw...Branch, Code 824, San Diego, CA Technical Report (pages 6-9, 10, 40) Sears, F. W. and M. W. Zemansky . 1955. University Physics. Second Edition, Addison

  12. Treatment of LF slag to prevent powdering during cooling

    Directory of Open Access Journals (Sweden)

    Ghorai S.

    2017-01-01

    Full Text Available The polymorphic transformation of the monoclinic β-polymorph to the orthorhombic γ-polymorph of di-calcium silicate at around 500°C during cooling results in disintegration of slag. The slag generated, during the production of thermo mechanically treated steel in ladle furnace at M/s Tata Steel Limited, Jamshedpur, India, behaves in similar manner. An attempt has been made to prevent the crumbling of ladle furnace slag. The experiments were conducted in 10 kg air induction furnace. Various types of silica source were used to prevent the disintegration of ladle furnace slag by reducing the basicity and optimizing the additives amount. Apart from silica sources, other additives like borax and barium carbonate were also used to stabilize the β phase. Present investigation reveals that disintegration of ladle furnace slag can be prevented either by addition of 0.2% boarx or 2% barium carbonate. Dust formation can also be prevented by decreasing the ladle furnace slag basicity to about 1.7. Toxicity Characteristic Leaching Procedure test, of the borax and barium carbonate treated slag samples, indicates that barium carbonate treated slag cannot be used for the dusting prevention as it contains high level of barium.

  13. VLF/LF Reflectivity of the Polar Ionosphere.

    Science.gov (United States)

    1980-06-01

    APPROVED: dk EDW1AD A. LEWIS Propagation Branch Electromagnetic Sciences Division APPROVED: ALLAN ~ C. SCHELL, Chief Electromagnetic Sciences Division...o0o o.s D ’ o~oo, 0l0 C’~3JI~0J .OIC U3 ’. - C,~jJ~jO~ .0t~]~3~ (0,cc 0 0 C? * v ocl )JI Cno f -oi i on i idw0 P Pt pop Poe e P-p. opt d73 S * A K A

  14. Phytochemical Observation on Leaf of Justicia Tranquebariesis. L.F.

    Science.gov (United States)

    Akilandeswari, S.; Mainmaran, S.; Valarmathi, R.; Kumara, S. Karpagam; Loganathan, Sundari V.

    2001-01-01

    Photochemical studies of leaf of the herbs Justicia tranquebariensis. (Acanthaceae) carried out in the presence of phytosterols, flavonoids, Glycosides and absence of triterpenoids, alkaloids, saponins tannins have been reported in this herb for the first time. PMID:22557013

  15. LF airport ground noise mitigation using scattering sections

    NARCIS (Netherlands)

    Bosschaart, C.; Eisses A.R.; Eerden, F.J.M. van der

    2012-01-01

    Ground noise due to aircraft starting from the Amsterdam airport runway ‘Polderbaan’ has been an issue since its construction in 2003. Take off operation in headwind conditions causes downwind sound propagation towards a residential area situated 2,5 km Southwest of the runway. Due to the large

  16. ELF/VLF/LF Propagation and System Design

    Science.gov (United States)

    1987-06-23

    values [681 to data of Rhoads and Garner 1691 for the path from Haiku to Washington, DC, that occurred in May and June 1965. They found a good fit to...Figs. 106 and 107. Experimental e/amples of the diurnal variation of received signal from Haiku to Washington, DC [691, are shown in Figs. 108 and 109

  17. LF airport ground noise mitigation using scattering sections

    NARCIS (Netherlands)

    Bosschaart, C.; Eisses A.R.; Eerden, F.J.M. van der

    2012-01-01

    Ground noise due to aircraft starting from the Amsterdam airport runway ‘Polderbaan’ has been an issue since its construction in 2003. Take off operation in headwind conditions causes downwind sound propagation towards a residential area situated 2,5 km Southwest of the runway. Due to the large prop

  18. Seleção de modelos polinomiais para representar o perfil e volume do fuste de Tectona grandis L.f. Selection of polynomial models to represent the profile and volume of the bole of Tectona grandisL.f.

    Directory of Open Access Journals (Sweden)

    Evandro Orfanó Figueiredo

    2006-12-01

    Full Text Available A precisão do volume de um povoamento florestal torna-se importante à medida que as empresas florestais integram verticalmente suas atividades e o resíduo da elaboração de um produto torna-se matéria-prima para outros. Os estudos realizados objetivaram avaliar a acurácia dos modelos polinomiais propostos por Schöepfer (1966, Hradetzky (1976 e Goulding & Murray (1976, na estimativa dos diâmetros e volumes ao longo do fuste de Tectona grandis L.f. de quatro povoamentos localizados na microrregião do Baixo Rio Acre e, ainda, testar a identidade do melhor modelo polinomial, avaliando-se a adequação de manter as áreas agrupadas ou segregá-las em grupos menores ou individualmente. A base de dados foi constituída de 159 árvores cubadas rigorosamente. Na avaliação da acurácia dos modelos foram empregadas estatísticas de desvio médio, desvio padrão das diferenças, soma dos quadrados dos resíduos relativos e resíduos percentuais. O modelo Goulding & Murray (1976 gerou as melhores estimativas de diâmetros e volumes ao longo do fuste, seguido pelos modelos Hradetzky (1976 e Schöepfer (1966. O teste de identidade de modelo mostrou ser mais adequado realizar ajustes independentes para as áreas 1 e 4 e para o subgrupo 2 e 3.The precise estimate of the volume of a forest stand become important as forest enterprises integrate vertically their activities and the residue of the manufacture of a product becomes the raw material for the manufacture of other. The accomplished studies aimed to evaluate the accuracy of the polynomial models proposed by Schöepfer (1966, Hradetzky (1976 and Goulding & Murray (1976 in the estimate of the diameters and volumes along the bole of Tectona grandis L.f from four stands situated in the Baixo Rio Acre micro region and further, to test the identity of the best polynomial model by evaluating the adequacy of maintaining the grouped areas or segregate them into smaller groups or singly. The data base was

  19. STUDY ON THE PHYSIOLOGY OF ENVIRONMENT POLLUTION AND THE EFFECT OF HEATING ON THE MALATHION RESIDUE ANALYZED USING THE RADIOTRACER METHOD

    Directory of Open Access Journals (Sweden)

    Razak Achmad Hamzah

    2011-04-01

    Full Text Available The aim of this study is to determine, the comparison of absorption by aquatic plants Hydrilla verticillata and Waterhyacinth (Eichhomia crassipes of malathion insecticide residues in water and comparison of malathion concentrationin tissues of fish fed with of contaminated water plants (Water hyacinth with tissues of fish, which was not fedcontaminated water plant. The effect of heating the contaminated fish tissue, on its level in tissues of rats that consumeit. For the first experiment (aquarium filled with 3 litre of water + H. verticillata 100 gr + Water hyacinth 100 gr + 20uci 14C-labeled malathion; for the second experiment (the first aquarium filled with 3 litre of water + 30 tails ofgoldfish + 20 uci 14C-labeled malathion; second aquarium filled with 3 litre of water + Water hyacinth 100 gr + 30 tailof goldfist + 20 uci 14C –labeled malathion. For the third experiment (most of contaminated fish tissue in the secondexperiment was dried at room temperature and then given to 30 mice and partly heated and then given to another 30mice. Malathion levels were then analyzed by using a liquid scintillation counter LSC-753 (Aloka. The results of alltreatments were compared using the Student t-test. It can be concluded, H. verticillata was more efficient compared tothe enceng gondok in absorbing the insecticide malathion residues in water; malathion concentration in the tissues offish fed Water hyacinth was higher than those of fish not fed Water hyacinth; contaminated fish tissue residues ofmalathion, although be heated, can not be lowered significantly, levels in the tissue.

  20. Combining ALS-Inhibiting Herbicides with the Fungal Pathogen Mycoleptodiscus terrestris for Control of Hydrilla

    Science.gov (United States)

    2009-07-01

    response to adverse environmental conditions. In fermentation , microsclerotia that develop in the broth medium as nutrients, particularly carbon and...these cases, recovery rates may have been delayed. Thirdly, Mt can produce secondary inoculum in the form of spores. The exact timing of this event

  1. Aquatic Plant Control Research Program. Moneoecious hydrilla in the Potomac River.

    Science.gov (United States)

    1985-08-01

    omy 24:29-96. Pulich, W. M., Jr. 1982. Edaphic factors related to shoalgrass ( Halodule Wrightii Aschers.) production. Botanica Marina 25:467-475...dwelling organisms. The best available data concerning the benthic macroinvertebrate fauna of the freshwater tidal portion of the Potomac were...oxygenators in rivers. Water Research 2:243-248. Egglishaw, H. J. 1964. The distributional relationship between the bottom fauna and plant detritus in

  2. 不同磷营养水平对2种沉水植物在Cd·Zn复合污染下的影响%Effects of Different Phosphorus Concentrations on Two Submerged Plants under Combined Pollution of Cd, Zn

    Institute of Scientific and Technical Information of China (English)

    张慧; 王超; 王沛芳; 薛艳

    2011-01-01

    [目的]揭示富营养化和重金属污染共同存在时对沉水植物的影响,为水生生态系统的健康评价和治理提供理论依据和手段.[方法]比较研究了不同磷营养水平对Cd、Zn复合污染下伊乐藻(Elodea nuttallii)、黑藻(Hydrilla verticillata)2种沉水植物叶绿素含量和叶绿素a/b比值、MDA含量、GSH含量、POD活性、CAT活性等生理生化指标的影响.[结果]随着培养时间延长,2种植物的叶绿素含量基本呈下降趋势,MDA含量呈先上升后下降趋势,POD活性及可溶性蛋白含量呈先下降后上升趋势,GSH呈缓慢上升趋势,当不存在磷水平时CAT活性无明显变化;但是黑藻比伊乐藻对胁迫更敏感,并且耐性优于伊乐藻.[结论]不同磷营养水平对受重金属胁迫植物的影响不一样,其中低磷水平(0.05 mg/L)可在一定程度上级解重金属的毒害,与重金属产生拮抗作用;而高磷水平(5 mg/L)则加重了重金属对植物的伤害,产生协同作用.%[ Objective ] The aim was to reveal effects of eutrophication on two kinds of submerged plant under heavy metal contamination, thus to provide theoretical basis for health evaluation and treatment measures of aquatic ecosystems. [ Method ] The effects of different phosphorus levels on the chlorophyll contents and a/b values of Elodea nuttallii and Hydrilla verticillata were compared and investigated. [ Result ] With the culture time prolonging, the chlorophyll contents of the two kinds of plant showed a general decreasing tendency ; MDA content was first increased and then decreased; POD activity and soluble protein content were furst decreased and then increased; GSH showed a slow upward trend; there was no significant change in CAT activity when no phosphorus existed; in addition, Elodea nuttallii had better sensitivity and tolerance to the stress than Hydrilla verticillata. [ Conclusion ] Effects of different phosphorus levels on the plants under heavy metal stress differed

  3. The Distribution of Submersed Aquatic Vegetation in the Fresh and Oligohaline Tidal Potomac River, 2005

    Science.gov (United States)

    Rybicki, Nancy B.; Justiniano-Velez, Erika M.; Schenk, Edward R.; Baldizar, Julie M.; Hunter, Sarah E.

    2008-01-01

    Submersed aquatic vegetation (SAV) is a critical component of the Potomac River ecosystem. Though SAV provides important habitat for fauna and stabilizes bottom sediment, very dense beds may restrict recreational and commercial navigation. Exotic species of SAV are managed by the Metropolitan Washington Council of Governments Potomac Aquatic Plant Management Program (PAPMP). Selected beds of primarily exotic SAV species that limit navigation are harvested mechanically. The program began in 1986 when approximately 40 acres of plants were harvested from 18 sites (Metropolitan Washington Council of Governments 1987). Monitoring efforts are an effective means of quantifying the distribution and abundance of the exotic species, Hydrilla verticillata (hydrilla) and other SAV species. These annual surveys provide a basis for identifying large-scale changes and trends throughout the ecosystem and allow managers to evaluate the effectiveness of resource management policies based on a reliable scientific foundation (Rybicki and Landwehr, 2007). The U.S. Geological Survey (USGS) has monitored the distribution and composition of SAV beds in the fresh and oligohaline (salinity 0.5 to 5) tidal Potomac River since 1978 using transect sampling (1978 to 1981, 1985 to 1987, and 2002) and shoreline surveys (1983 to 2005). The Government of the District of Columbia has monitored the portion of the Potomac and Anacostia Rivers within Washington DC since 1998 (Rottman, 1999; Ryan, 2005, 2006). The species of SAV observed in beds in the tidal Potomac River are incorporated into the Virginia Institute of Marine Science (VIMS) annual report on SAV distribution in Chesapeake Bay. The VIMS report and methods are available at http://www.vims.edu/bio/sav (Orth et al., 2006). Additional publications concerning SAV distribution in the Potomac River can be found at http://water.usgs.gov/nrp/proj.bib/sav/wethome.htm.

  4. 上海后滩湿地沉水植物群落系统对底泥的生态修复效应%On the effects of the submerged macrophyte community systems on the sedimentary restoration of Shanghai Houtan wetland

    Institute of Scientific and Technical Information of China (English)

    董悦; 霍姮翠; 谢文博; 杜佳沐; 罗思亭; 王聪; 张饮江

    2013-01-01

    利用5种不同沉水植物群落系统对中国2010上海世博区后滩湿地底泥进行生态修复,于2009年8月-2010年8月对沉水植物覆盖度与生物量,以及底泥有机质(0M)、总氮(TN)、总磷(TP)的分布与变化进行动态监测.结果表明:沉水植物群落覆盖度、生物量有明显的季节变化,总体呈升高趋势,且与底泥OM、TN、TP呈正相关;底泥经修复后,OM质量分数、TN和TP质量比分别比背景值降低61.9%~79.7%、78.7%~ 83.9%、32.3%~42.7%;底泥有机指数从Ⅳ级(有机污染)降至Ⅱ级(较清洁),降低了水体富营养化的风险;2010年8月各净化区沉水植物群落数量特征均高于2009年8月,l区的伊乐藻(Elodea nuttallii)、狐尾藻(Myriophyllumverticillatum)、轮叶黑藻(Hydrilla verticillata)、金鱼藻(Ceratophyllum demersum L.)群落以及2区的苦草(Vallisneria natans(Lour.) Hara)、金鱼藻(Ceratophyllum demersum L.)、轮叶黑藻(Hydrilla verticillata)对营养物的去除效果较其他净化区显著;底泥OM质量分数、TN质量比在垂直的0~30 cm内分布较一致,TP质量比在0~15 cm内降低,在15 ~ 30 cm内升高.研究表明,后滩湿地沉水植物群落系统对底泥营养盐修复效果明显,沉水植物群落逐渐趋于稳定,具有一定可持续性.

  5. Studies on sustainability of simulated constructed wetland system for treatment of urban waste: Design and operation.

    Science.gov (United States)

    Upadhyay, A K; Bankoti, N S; Rai, U N

    2016-03-15

    New system configurations and wide range of treatability make constructed wetland (CW) as an eco-sustainable on-site approach of waste management. Keeping this view into consideration, a novel configured three-stage simulated CW was designed to study its performance efficiency and relative importance of plants and substrate in purification processes. Two species of submerged plant i.e., Potamogeton crispus and Hydrilla verticillata were selected for this study. After 6 months of establishment, operation and maintenance of simulated wetland, enhanced reduction in physicochemical parameters was observed, which was maximum in the planted CW. The percentage removal (%) of the pollutants in three-stage mesocosms was; conductivity (60.42%), TDS (67.27%), TSS (86.10%), BOD (87.81%), NO3-N (81.28%) and PO4-P (83.54%) at 72 h of retention time. Submerged macrophyte used in simulated wetlands showed a significant time dependent accumulation of toxic metals (p ≤ 0.05). P. crispus accumulated the highest Mn (86.36 μg g(-1) dw) in its tissue followed by Cr (54.16 μg g(-1) dw), Pb (31.56 μg g(-1) dw), Zn (28.06 μg g(-1) dw) and Cu (25.76 μg g(-1) dw), respectively. In the case of H. verticillata, it was Zn (45.29), Mn (42.64), Pb (22.62), Cu (18.09) and Cr (16.31 μg g(-1) dw). Thus, results suggest that the application of simulated CW tackles the water pollution problem more efficiently and could be exploited in small community level as alternative and cost effective tools of phytoremediation.

  6. The growth of three submerged plants in different polluted water and its impact on water quality%3种沉水植物在不同污染水体中的生长及其对水质的影响

    Institute of Scientific and Technical Information of China (English)

    吴旻; 赵群芬

    2015-01-01

    In this paper, three submerged plants (goldfish algae(Ceratophyllum demersum L.), bitter grass(Hydrilla verticillata)and hydrilla(Vallisneria natans (Lour.) Hara)) were chosen as the research object and placed in different polluted water to explore different submerged plant’s purification effect on the water quality by monitoring water’s total nitrogen, total phosphorus. chlorophyll a content, and pH, etc. At the same time, based on the total nitrogen and total phosphorus content in plant and superoxide enzyme (SOD), malondialdehyde (MDA), the growth of the plants in different polluted water was researched. The results showed that the purification effects of different submerged plants have a significant difference on different polluted water, and the stress of different polluted water on the physical growth of submerged plant also has different effects.%以金鱼藻(Ceratophyllum demersum L.)、苦草(Hydrilla verticillata)、黑藻(Vallisneria natans (Lour.) Hara)3种沉水植物作为研究对象,分别将其放置于不同污染水体中培养,通过监测水体总氮、总磷、叶绿素a含量和pH值等,探究不同沉水植物对水质的净化效果;同时通过对植物体内总氮、总磷含量以及超氧化物酶(SOD)、丙二醛(MDA)的变化,研究植物在不同污染水体中的生长情况。结果表明,不同沉水植物对不同污染水体的净水效果有着显著差异,不同污染水体的胁迫作用对沉水植物本身的生理生长也有着不同的影响。综合所研究的沉水植物的净水优势以及在不同污染水体中的适应情况,筛选出在各种污染水体中的优势种,归纳总结出生态修复组合的方案。

  7. Biological and Host Range Studies with Bagous affinis, An Indian Weevil that Destroys Hydrilla Tubers. Aquatic Plant Control Research Program.

    Science.gov (United States)

    1994-12-01

    of Entomology and Nematology Institute of Food and Agriculture Services University of Florida Gainesville, FL 32606 Final report Approved for public...FL 32614-7100 Department of Entomology and Nematology , Institute of Food and Agriculture Services, University of Florida, Gainesville, FL 32606 9

  8. ELF/VLF/LF Radio Propagation and Systems Aspects (La Propagation des Ondes Radio ELF/VLF/LF et les Aspects Systemes)

    Science.gov (United States)

    1993-05-01

    Umime 11uld sb’ngft R11111M fgM 7-1 INVESTIGATIONS OF EQUATORIAL IONOSPHERE NIGHTTIME MODE CONVERSION AT VLF* Verne Hildebrand IWG Corporation, 1940 Fifth...uniform, Symposium), supposing that has an length equal to Ŗa" and it is MIT, Cambridge oriented on the zz direction (fig.8). USA, Julio 1991 Let us...about 10 to 14 years. My from the floor, would someone like to start off? own experience as a systems engineer is that Verne Hildebrand the systems have

  9. FENOLOGÍA FLORAL Y VISITANTES FLORALES EN Drimys granadensis L.f. (WINTERACEAE Floral Phenology And Flower Visitors In Drimys granadensis L.f. (Winteraceae

    Directory of Open Access Journals (Sweden)

    XAVIER MARQUINEZ

    Full Text Available El propósito de este trabajo fue caracterizar las fases fenológicas florales y determinar los visitantes florales en una población natural de Drimys granadensis (nombre común: canelo; Winteraceae ubicada en Altos de Yerbabuena ( 2.850 m , cerros orientales de la Sabana de Bogotá, (Colombia. El desarrollo fenológico floral duró 9,5 días cuando la floración ocurrió en época soleada, 12,5 días en época lluviosa y 16 días cuando se excluyeron a los visitantes florales mediante embolsado. Se realizaron observaciones del recurso utilizado, fase fenólogica de la flor visitada y cargas polínicas en los visitante florales, los cuales correspondieron a seis órdenes, 21 familias y 29 morfoespecies de insectos. Cuatro especies de coleópteros y dos de dípteros fueron considerados posibles polinizadores por su abundancia y carga de polen. Los resultados obtenidos se discuten en relación con aquellos reportados en otras especies del género Drimys y de la familia Winteraceae.The purpose of this research was to characterize the flowering phenological phases and to determine the flower visitors in a natural population of Drimys granadensis (common name: canelo; Winteraceae in Altos de Yerbabuena, eastern mountains of Sabana de Bogotá ( Colombia . Floral phenology development lasted 9.5 days when flowering occurred under sunny conditions, 12.5 days under rainy conditions and 16 days when flower visitors were excluded using cloth bags. It was done observations related with the resources used, the phenological phases of visited flowers and the pollen load on the flower visitors. Visitors corresponded with 29 morphospecies, 6 orders y 21 families of insects. Four species of coleoptera and two species of diptera were considered as possible pollinators taking in account abundance and pollen load. The results are analyzed in relation with those reported for other species in the genus Drimys and in the family Winteraceae.

  10. Water quality effects of two aquatic macrophytes on eutrophic water from Lake Dianchi Caohai%两种水生植物对滇池草海富营养化水体水质的影响

    Institute of Scientific and Technical Information of China (English)

    王智; 张志勇; 张君倩; 闻学政; 王岩; 刘海琴; 严少华

    2013-01-01

    The water quality effects of two typical aquatic macrophytes, Eichhornia crassipes and Hydrilla verticillata, on the eutrophic water from Lake Dianchi Caohai were investigated by a series of microcosm experiments. The assimilation of nitrogen and phosphorus from microcosm by E. crassipes were 109% and 17% higher than that by H. verticillata, respectively. The leves of dissovle oxygen and pH in the E. crassipes treatments were significantly reduced, and electric conduction and redox potential were significantly increased comparing with the control. However, the results of H. verticillata treatments were opposive to E. crassipes treatments. During the experiment, the concentrations of total nitrogen (TN) and total phosphorus (TP) in treatments were significantly lower than that in control. With the same biomass of two aquatic plants used at the beginning of the experiment, the concentrations of TN, TP and chlorophyll-a (Chl-a) in H. verticillata treatments were significantly lower than that in E. crassipes treatments in the early stage of the experiment, but the results were reversed in the late stage of the experiment. After aquatic macrophytes harvest, concentrations of TN and TP could maintain the levels of which before harvest; Chl-a concentrations in E. crassipes treatments could also maintain the level of which before harvest, but in H. verticillata treatments, Chl-a concentrations showed a significant increase.%通过模拟实验,比较了2种典型水生植物水葫芦和轮叶黑藻对滇池草海富营养化水体水质的影响.结果表明,在实验过程中,水葫芦同化吸收的氮、磷量分别比轮叶黑藻所同化吸收的量高109%和17%.在水生植物采收前,水葫芦处理组水体DO和pH值显著性低于对照组,电导率(EC)和氧化还原电位(Eh)显著性高于对照组,与轮叶黑藻处理组结果相反.水葫芦和轮叶黑藻处理组水体TN和TP浓度均显著低于对照组;相同初始种养量的情况下,试验初

  11. Eutrophication increases methane emission to the atmosphere in tropical lagoons: insights from two Ivory Coast sites

    Science.gov (United States)

    José-mathieu Koné, Yéfanlan; Vieira Borges, Alberto

    2017-04-01

    Eutrophication increases methane emission to the atmosphere in tropical lagoons: insights from two Ivory Coast sites. Y J M Koné (1) & A.V. Borges (2) (1) Centre de recherches océanologiques (CRO) d'Abidjan, (Ivory Coast) (2) University of Liège, Chemical Oceanography Unit, Liège, Belgium (Belgium) Eutrophication is a worldwide environmental problem and a definitive solution is far from being achieved, despite the large number of studies documenting its causes. In small aquatic ecosystems, excessive growth of macrophytes is a well known undesirable consequence of eutrophication. When these plants die and sink to the bottom the decomposing biomass depletes oxygen content in the water column thus leading to anoxia promoting methane (CH4) production. Here, we reported the CH4 data obtained during six campaigns covering the annual cycle in two small lagoons of Ivory Coast (Ono, Kodjoboué) that are contrasted in the degree of eutrophication and the corresponding coverage of macrophytes (e.g. Echinochloa pyramidalis, Eichhornia crassipes, Hydrilla verticillata). Our data showed a high spatio-temporal variability of CH4 within the lagoons and between the two systems, with CH4 concentrations in surface waters ranging between 80 to 74,604 nmol L-1. The highest CH4 concentration values were observed in the eutrophic Ono lagoon that is covered by 80% of macrophytes, suggesting that lagoons dominated by macrophytes are significant sources of CH4 toward the atmosphere.

  12. Physico-chemical assessment of paper mill effluent and its heavy metal remediation using aquatic macrophytes--a case study at JK Paper mill, Rayagada, India.

    Science.gov (United States)

    Mishra, Swayamprabha; Mohanty, Monalisa; Pradhan, Chinmay; Patra, Hemanta Kumar; Das, Ritarani; Sahoo, Santilata

    2013-05-01

    The present investigation aims to assess the phytoremediation potential of six aquatic macrophytes, viz. Eichhornia crassipes, Hydrilla verticillata, Jussiaea repens, Lemna minor, Pistia stratiotes and Trapa natans grown in paper mill effluent of JK Paper mill of Rayagada, Orissa, for remediation of heavy metals. The experiment was designed in pot culture experiments. Assessment of physico-chemical parameters of paper mill effluent showed significant decrease in pH, conductivity, total dissolved solids, total suspended solids, chlorine, sulphur, biological and chemical oxygen demand after growth of macrophytes for 20 days. Phytoremediation ability of these aquatic macrophytic species for copper (Cu) and mercury (Hg) was indicated by assessing the decrease in the levels of heavy metals from effluent water. Maximum reduction (66.5 %) in Hg content of untreated paper mill effluent was observed using L. minor followed by T. natans (64.8 %). L. minor showed highest reduction (71.4 %) of Cu content from effluent water followed by E. crassipes (63.6 %). Phytoextraction potential of L. minor was remarkable for Hg and Cu, and bioaccumulation was evident from bioconcentration factor values, i.e. 0.59 and 0.70, respectively. The present phytoremediation approach was considered more effective than conventional chemical treatment method for removing toxic contaminants from paper mill effluent.

  13. Concentrations of heavy metals and aquatic macrophytes of Govind Ballabh Pant Sagar an anthropogenic lake affected by coal mining effluent.

    Science.gov (United States)

    Mishra, Virendra Kumar; Upadhyay, Alka Rani; Pandey, Sudhir Kumar; Tripathi, B D

    2008-06-01

    Five heavy metals Cu, Cd, Mn, Pb and Hg were found in high concentration from three sampling sites located in Asia's largest anthropogenic lake Govind Ballabh Pant GBP Sagar. Concentrations of these heavy metals were measured in Water, bottom sediment and in different parts of the aquatic macrophytes collected from the reservoir. Plants collected from the lake were Eichhornia crassipes, Azolla pinnata, Lemna minor, Spirodela polyrrhiza, Potamogeton pectinatus, Marsilea quadrifolia, Pistia stratiotes, Ipomea aquqtica, Potamogeton crispus, Hydrilla verticillata and Aponogeton natans. These plants have shown the high concentrations of Cu, Cd, Mn, Pb and Hg in their different parts due to bioaccumulation. In general plant roots exhibited higher concentrations of heavy metals than corresponding sediments. A comparison between different morphological tissues of the sampled plants revealed the metal concentration in following order roots > leaves. Analyses of bottom sediment indicated the higher concentrations of Cd, Mn, Cu and Pb. Strong positive correlations were obtained between the metals in water and in plants as well as between metal in sediment and in plants. Indicating the potential of these plants for pollution monitoring of these metals.

  14. Applicability of drinking water treatment residue for lake restoration in relation to metal/metalloid risk assessment

    Science.gov (United States)

    Yuan, Nannan; Wang, Changhui; Pei, Yuansheng; Jiang, Helong

    2016-12-01

    Drinking water treatment residue (DWTR), a byproduct generated during potable water production, exhibits a high potential for recycling to control eutrophication. However, this beneficial recycling is hampered by unclear metal/metalloid pollution risks related to DWTR. In this study, the pollution risks of Al, As, Ba, Be, Cd, Co, Cr, Cu, Fe, Mn, Mo, Ni, Pb, and Zn due to DWTR application were first evaluated for lake water based on human health risk assessment models and comparison of regulatory standards. The risks of DWTR were also evaluated for sediments on the basis of toxicity characteristics leaching procedure and fractionation in relation to risk assessment code. Variations in the biological behaviors of metal/metalloid in sediments caused by DWTR were assessed using Chironomus plumosus larvae and Hydrilla verticillata. Kinetic luminescent bacteria test (using Aliivibrio fischeri) was conducted to analyze the possibility of acute and chronic detrimental effects of sediment with DWTR application. According to the obtained results, we identify a potential undesirable effect of DWTR related to Fe and Mn (typically under anaerobic conditions); roughly present a dosage threshold calculation model; and recommend a procedure for DWTR prescreening to ensure safe application. Overall, managed DWTR application is necessary for successful eutrophication control.

  15. Enhancement of nitrate removal at the sediment-water interface by carbon addition plus vertical mixing.

    Science.gov (United States)

    Chen, Xuechu; He, Shengbing; Zhang, Yueping; Huang, Xiaobo; Huang, Yingying; Chen, Danyue; Huang, Xiaochen; Tang, Jianwu

    2015-10-01

    Wetlands and ponds are frequently used to remove nitrate from effluents or runoffs. However, the efficiency of this approach is limited. Based on the assumption that introducing vertical mixing to water column plus carbon addition would benefit the diffusion across the sediment-water interface, we conducted simulation experiments to identify a method for enhancing nitrate removal. The results suggested that the sediment-water interface has a great potential for nitrate removal, and the potential can be activated after several days of acclimation. Adding additional carbon plus mixing significantly increases the nitrate removal capacity, and the removal of total nitrogen (TN) and nitrate-nitrogen (NO3(-)-N) is well fitted to a first-order reaction model. Adding Hydrilla verticillata debris as a carbon source increased nitrate removal, whereas adding Eichhornia crassipe decreased it. Adding ethanol plus mixing greatly improved the removal performance, with the removal rate of NO3(-)-N and TN reaching 15.0-16.5 g m(-2) d(-1). The feasibility of this enhancement method was further confirmed with a wetland microcosm, and the NO3(-)-N removal rate maintained at 10.0-12.0 g m(-2) d(-1) at a hydraulic loading rate of 0.5 m d(-1).

  16. Permanent Genetic Resources added to Molecular Ecology Resources Database 1 May 2009-31 July 2009.

    Science.gov (United States)

    Almany, Glenn R; DE Arruda, Maurício P; Arthofer, Wolfgang; Atallah, Z K; Beissinger, Steven R; Berumen, Michael L; Bogdanowicz, S M; Brown, S D; Bruford, Michael W; Burdine, C; Busch, Jeremiah W; Campbell, Nathan R; Carey, D; Carstens, Bryan C; Chu, K H; Cubeta, Marc A; Cuda, J P; Cui, Zhaoxia; Datnoff, L E; Dávila, J A; Davis, Emily S; Davis, R M; Diekmann, Onno E; Eizirik, Eduardo; Fargallo, J A; Fernandes, Fabiano; Fukuda, Hideo; Gale, L R; Gallagher, Elizabeth; Gao, Yongqiang; Girard, Philippe; Godhe, Anna; Gonçalves, Evonnildo C; Gouveia, Licinia; Grajczyk, Amber M; Grose, M J; Gu, Zhifeng; Halldén, Christer; Härnström, Karolina; Hemmingsen, Amanda H; Holmes, Gerald; Huang, C H; Huang, Chuan-Chin; Hudman, S P; Jones, Geoffrey P; Kanetis, Loukas; Karunasagar, Iddya; Karunasagar, Indrani; Keyghobadi, Nusha; Klosterman, S J; Klug, Page E; Koch, J; Koopman, Margaret M; Köppler, Kirsten; Koshimizu, Eriko; Krumböck, Susanne; Kubisiak, T; Landis, J B; Lasta, Mario L; Lee, Chow-Yang; Li, Qianqian; Li, Shou-Hsien; Lin, Rong-Chien; Liu, M; Liu, Na; Liu, W C; Liu, Yuan; Loiseau, A; Luan, Weisha; Maruthachalam, K K; McCormick, Helen M; Mellick, Rohan; Monnahan, P J; Morielle-Versute, Eliana; Murray, Tomás E; Narum, Shawn R; Neufeld, Katie; De Nova, P J G; Ojiambo, Peter S; Okamoto, Nobuaki; Othman, Ahmad Sofiman; Overholt, W A; Pardini, Renata; Paterson, Ian G; Patty, Olivia A; Paxton, Robert J; Planes, Serge; Porter, Carolyn; Pratchett, Morgan S; Püttker, Thomas; Rasic, Gordana; Rasool, Bilal; Rey, O; Riegler, Markus; Riehl, C; Roberts, John M K; Roberts, P D; Rochel, Elisabeth; Roe, Kevin J; Rossetto, Maurizio; Ruzzante, Daniel E; Sakamoto, Takashi; Saravanan, V; Sarturi, Cladinara Roberts; Schmidt, Anke; Schneider, Maria Paula Cruz; Schuler, Hannes; Serb, Jeanne M; Serrão, Ester T A; Shi, Yaohua; Silva, Artur; Sin, Y W; Sommer, Simone; Stauffer, Christian; Strüssmann, Carlos Augusto; Subbarao, K V; Syms, Craig; Tan, Feng; Tejedor, Eugenio Daniel; Thorrold, Simon R; Trigiano, Robert N; Trucco, María I; Tsuchiya-Jerep, Mirian Tieko Nunes; Vergara, P; Van De Vliet, Mirjam S; Wadl, Phillip A; Wang, Aimin; Wang, Hongxia; Wang, R X; Wang, Xinwang; Wang, Yan; Weeks, Andrew R; Wei, Fuwen; Werner, William J; Wiley, E O; Williams, D A; Wilkins, Richard J; Wisely, Samantha M; With, Kimberly A; Wu, Danhua; Yao, Cheng-Te; Yau, Cynthia; Yeap, Beng-Keok; Zhai, Bao-Ping; Zhan, Xiangjiang; Zhang, Guo-Yan; Zhang, S Y; Zhao, Ru; Zhu, Lifeng

    2009-11-01

    This article documents the addition of 512 microsatellite marker loci and nine pairs of Single Nucleotide Polymorphism (SNP) sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Alcippe morrisonia morrisonia, Bashania fangiana, Bashania fargesii, Chaetodon vagabundus, Colletes floralis, Coluber constrictor flaviventris, Coptotermes gestroi, Crotophaga major, Cyprinella lutrensis, Danaus plexippus, Fagus grandifolia, Falco tinnunculus, Fletcherimyia fletcheri, Hydrilla verticillata, Laterallus jamaicensis coturniculus, Leavenworthia alabamica, Marmosops incanus, Miichthys miiuy, Nasua nasua, Noturus exilis, Odontesthes bonariensis, Quadrula fragosa, Pinctada maxima, Pseudaletia separata, Pseudoperonospora cubensis, Podocarpus elatus, Portunus trituberculatus, Rhagoletis cerasi, Rhinella schneideri, Sarracenia alata, Skeletonema marinoi, Sminthurus viridis, Syngnathus abaster, Uroteuthis (Photololigo) chinensis, Verticillium dahliae, Wasmannia auropunctata, and Zygochlamys patagonica. These loci were cross-tested on the following species: Chaetodon baronessa, Falco columbarius, Falco eleonorae, Falco naumanni, Falco peregrinus, Falco subbuteo, Didelphis aurita, Gracilinanus microtarsus, Marmosops paulensis, Monodelphis Americana, Odontesthes hatcheri, Podocarpus grayi, Podocarpus lawrencei, Podocarpus smithii, Portunus pelagicus, Syngnathus acus, Syngnathus typhle,Uroteuthis (Photololigo) edulis, Uroteuthis (Photololigo) duvauceli and Verticillium albo-atrum. This article also documents the addition of nine sequencing primer pairs and sixteen allele specific primers or probes for Oncorhynchus mykiss and Oncorhynchus tshawytscha; these primers and assays were cross-tested in both species.

  17. Mesohaline submerged aquatic vegetation survey along the U.S. gulf of Mexico coast, 2001 and 2002: A salinity gradient approach

    Science.gov (United States)

    Merino, J.H.; Carter, J.; Merino, S.L.

    2009-01-01

    Distribution of marine submerged aquatic vegetation (SAV; i.e., seagrass) in the northern Gulf of Mexico coast has been documented, but there are nonmarine submersed or SAV species occurring in estuarine salinities that have not been extensively reported. We sampled 276 SAV beds along the gulf coast in Florida, Alabama, Mississippi, Louisiana, and Texas in 2001 and 2002 in oligohaline to polyhaline (0 to 36 parts per thousand) waters to determine estuarine SAV species distribution and identify mesohaline SAV communities. A total of 20 SAV and algal species was identified and habitat characteristics such as salinity, water depth, pH, conductivity, turbidity, dissolved oxygen, and sediment composition were collected. Fourteen SAV species occurred two or more times in our samples. The most frequently occurring species was Ruppia maritima L. (n = 148), occurring in over half of SAV beds sampled. Eleocharis sp. (n = 47), characterized with an emergent rather than submerged growth form, was a common genus in the SAV beds sampled. A common marine species was Halodule wrightii Asch. (n = 36). Nonindigenous species Myriophyllum spicatum L. (n = 31) and Hydrilla verticillata (L. f.) Royle (n = 6) were present only in oligohaline water. Analyzing species occurrence and environmental characteristics using canonical correspondence and two-way indicator species analysis, we identify five species assemblages distinguished primarily by salinity and depth. Our survey increases awareness of nonmarine SAV as a natural resource in the gulf, and provides baseline data for future research. ?? 2009 by the Marine Environmental Sciences Consortium of Alabama.

  18. Dark/light modulation of ribulose bisphosphate carboxylase activity in plants from different photosynthetic categories

    Energy Technology Data Exchange (ETDEWEB)

    Vu, J.C.V.; Allen, L.H. Jr.; Bowes, G.

    1984-11-01

    Ribulose bisphosphate carboxylase/oxygenase (RuBPCase) from several plants had substantially greater activity in extracts from light-exposed leaves than dark leaves, even when the extracts were incubated in vitro with saturating HCO/sub 3//sup -/ and Mg/sup 2 +/ concentrations. This occurred in Glycine max, Lycopersicon esculentum, Nicotiana tabacum, Panicum bisulcatum, and P. hylaeicum (C/sub 3/); P. maximum (C/sub 4/ phosphoenolpyruvate carboxykinase); P. milioides (C/sub 3//C/sub 4/); and Bromelia pinguin and Ananas comosus (Crassulacean acid metabolism). Little or no difference between light and dark leaf extracts of RuBPCase was observed in Triticum aestivum (C/sub 3/); P. miliaceum (C/sub 4/ NAD malic enzyme); Zea mays and Sorghum bicolor (C/sub 4/ NADP malic enzyme); Moricandia arvensis (C/sub 3//C/sub 4/); and Hydrilla verticillata (submersed aquatic macrophyte). It is concluded that, in many plants, especially Crassulacean acid metabolism and C/sub 3/ species, a large fraction of ribulose-1,5-bisphosphate carboxylase/oxygenase in the dark is in an inactivatable state that cannot respond to CO/sub 2/ and Mg/sup 2 +/ activation, but which can be converted to an activatable state upon exposure of the leaf to light. 16 references, 2 tables.

  19. Dark/Light Modulation of Ribulose Bisphosphate Carboxylase Activity in Plants from Different Photosynthetic Categories 1

    Science.gov (United States)

    Vu, J. Cu V.; Allen, Leon H.; Bowes, George

    1984-01-01

    Ribulose bisphosphate carboxylase/oxygenase (RuBPCase) from several plants had substantially greater activity in extracts from lightexposed leaves than dark leaves, even when the extracts were incubated in vitro with saturating HCO3− and Mg2+ concentrations. This occurred in Glycine max, Lycopersicon esculentum, Nicotiana tabacum, Panicum bisulcatum, and P. hylaeicum (C3); P. maximum (C4 phosphoenolpyruvate carboxykinase); P. milioides (C3/C4); and Bromelia pinguin and Ananas comosus (Crassulacean acid metabolism). Little or no difference between light and dark leaf extracts of RuBPCase was observed in Triticum aestivum (C3); P. miliaceum (C4 NAD malic enzyme); Zea mays and Sorghum bicolor (C4 NADP malic enzyme); Moricandia arvensis (C3/C4); and Hydrilla verticillata (submersed aquatic macrophyte). It is concluded that, in many plants, especially Crassulacean acid metabolism and C3 species, a large fraction of ribulose-1,5-bisphosphate carboxylase/oxygenase in the dark is in an inactivatable state that cannot respond to CO2 and Mg2+ activation, but which can be converted to an activatable state upon exposure of the leaf to light. PMID:16663937

  20. Dark/Light modulation of ribulose bisphosphate carboxylase activity in plants from different photosynthetic categories.

    Science.gov (United States)

    Vu, J C; Allen, L H; Bowes, G

    1984-11-01

    Ribulose bisphosphate carboxylase/oxygenase (RuBPCase) from several plants had substantially greater activity in extracts from lightexposed leaves than dark leaves, even when the extracts were incubated in vitro with saturating HCO(3) (-) and Mg(2+) concentrations. This occurred in Glycine max, Lycopersicon esculentum, Nicotiana tabacum, Panicum bisulcatum, and P. hylaeicum (C(3)); P. maximum (C(4) phosphoenolpyruvate carboxykinase); P. milioides (C(3)/C(4)); and Bromelia pinguin and Ananas comosus (Crassulacean acid metabolism). Little or no difference between light and dark leaf extracts of RuBPCase was observed in Triticum aestivum (C(3)); P. miliaceum (C(4) NAD malic enzyme); Zea mays and Sorghum bicolor (C(4) NADP malic enzyme); Moricandia arvensis (C(3)/C(4)); and Hydrilla verticillata (submersed aquatic macrophyte). It is concluded that, in many plants, especially Crassulacean acid metabolism and C(3) species, a large fraction of ribulose-1,5-bisphosphate carboxylase/oxygenase in the dark is in an inactivatable state that cannot respond to CO(2) and Mg(2+) activation, but which can be converted to an activatable state upon exposure of the leaf to light.

  1. Screening of native plants and algae growing on fly-ash affected areas near National Thermal Power Corporation, Tanda, Uttar Pradesh, India for accumulation of toxic heavy metals.

    Science.gov (United States)

    Dwivedi, S; Srivastava, S; Mishra, S; Dixit, B; Kumar, A; Tripathi, R D

    2008-10-30

    The present investigation was carried out to screen native plants growing in fly-ash (FA) contaminated areas near National Thermal Power Corporation, Tanda, Uttar Pradesh, India with a view to using them for the eco-restoration of the area. A total number of 17 plants (9 aquatic, 6 terrestrial and 2 algal species) were collected and screened for heavy metal (Fe, Zn, Cu, Mo, B, Si, Al, Cr, Pb, Cd, Hg and As) accumulation. Differential accumulation of various heavy metals by different species of plants was observed. Hydrilla verticillata was found to be the most efficient metal accumulator among 9 aquatic plants, Eclipta alba among 6 terrestrial plants and Phormedium papyraceum between 2 algal species. In general, the maximum levels of most metals were found in terrestrial plants while the lowest in algal species. However, translocation of the metals from root to shoot was found to be higher in aquatic plants than terrestrial ones. These results suggest that various aquatic, terrestrial and algal species of plants may be used in a synergistic way to remediate and restore the FA contaminated areas.

  2. Purification and Influence of Submerged Plants on Water Quality of Landscape Water Body Supplied by Reclaimed Water%沉水植物对再生水景观水体水质变化的影响和净化效果

    Institute of Scientific and Technical Information of China (English)

    徐志嫱; 刘维; 赵洁; 刘玉玲

    2012-01-01

    This research is to investigate the eutrophication change and water quality improvement by submerged plants in a landscape pond supplied by reclaimed water.An artificial lake of Xi'an was chosen to monitor the water quality at different locations and periods.An outdoor study was carried out to research the effects of nutrient removal by Elodea canadensis and Hydrilla verticillata in reclaimed water.The results showed that concentrations of ammonia nitrogen(NH+4-N) and total nitrogen(TN) were clearly lower than the open water area,and the water was in low eutrophication state in the area with submerged plants,mainly located at the edge of the lake.Elodea canadensis and Hydrilla verticillata showed a perfect effect for reclaimed water quality.The removal rates of Elodea canadensis of CODCr,TN and TP were 38.55%~57.54%,43.13%~51.81% and 61.76%~77.45% respectively,that were superior to Hydrilla verticillata's.Elodea canadensis had inhibitory effect on algae,and chlorophyll a kept in 20 μg/L level during long time.It was suggested that Elodea canadensis should be used as the pioneer plants for restoration of landscape water body supplied by reclaimed water in the northwest area.%为揭示沉水植物对以再生水作为水源的景观水体富营养化变化趋势的影响和对水体水质的维持和改善作用,对西安市一个再生水景观水体开展了不同区域和不同季节的水质监测试验,并采用室外玻璃缸装置研究伊乐藻和轮叶黑藻对再生水中氮、磷等营养盐的去除效果。结果表明:有沉水植物生长的区域氨氮、总氮浓度明显低于主湖区,且水质能够维持在低富营养化状态。伊乐藻和轮叶黑藻对再生水水质具有良好的改善作用,且伊乐藻优于轮叶黑藻。伊乐藻对再生水中CODCr、氮、磷的去除率分别为38.55%~57.54%,43.13%~51.81%和61.76%~77.45%,可使水体中叶绿素a含量维持在20μg/L左右,对藻类具有较好的抑制作

  3. Ex vivo intestinal adhesion of Escherichia coli LF82 in Crohn's disease

    DEFF Research Database (Denmark)

    Jensen, Stina Rikke; Fink, Lisbeth Nielsen; Nielsen, Ole Haagen

    2011-01-01

    Adherent-invasive Escherichia coli (AIEC) are reported to inhabit the gut mucosa in Crohn's disease (CD), however, little is known about the importance of host factors for the interplay between AIEC and the human gut. To examine if differences in bacterial adhesion patterns are disease associated...

  4. Stored Radioactive Material Landfill Site no. 7 (LF-7), Massachusetts Military Reservation, Cape Cod, MA

    Science.gov (United States)

    2013-03-07

    site. The surface area was characterized utilizing two 2x2 sodium iodide (NaI) detectors using a gamma walkover technique, one RS-700 for both in...calibrated with natural thorium and a (10-μCi) Cs-137 source on the first day of sampling. According to the manufacturer, gain stabilization is...accomplished using natural uranium, thorium, and potassium when necessary. The team also performed operational checks utilizing Co-60 prior to field work and

  5. l/f Noise in the Superconducting Transition of a MgB2 Thin Film

    Science.gov (United States)

    Lakew, B.; Aslam, S.; Jones, H.; Stevenson, T.; Cao, N.

    2010-01-01

    The noise voltage spectral density in the superconducting transition of a MgB2 thin film on a SiN-coated Si thick substrate was measured over the frequency range 1 Hz-to-1 KHz. Using established bolometer noise theory the theoretical noise components due to Johnson, 1/f(excess) and phonon noise are modeled to the measured data. It is shown that for the case of a MgB2 thin film in the vicinity of the mid-point of transition, coupled to a heat sink via a fairly high thermal conductance (approximately equal to 10(sup -1) W/K)) that the measured noise voltage spectrum is 1/f limited and exhibits lit dependence with a varying between 0.3 and 0.5 in the measured frequency range. At a video frame rate frequency of 30 Hz the measured noise voltage density in the film is approximately equal to 61 nV /the square root of HZ, using this value an upper limit of electrical NEP approximately equal to 0.67pW / the square root of Hz is implied for a practical MgB2 bolometer operating at 36.1 K.

  6. High-latitude propagation studies using a meridional chain of LF/MF/HF receivers

    Directory of Open Access Journals (Sweden)

    J. LaBelle

    2004-04-01

    Full Text Available For over a decade, Dartmouth College has operated programmable radio receivers at multiple high-latitude sites covering the frequency range 100-5000kHz with about a 1-s resolution. Besides detecting radio emissions of auroral origin, these receivers record characteristics of the ionospheric propagation of natural and man-made signals, documenting well-known effects, such as the diurnal variation in the propagation characteristics of short and long waves, and also revealing more subtle effects. For example, at auroral zone sites in equinoctial conditions, the amplitudes of distant transmissions on MF/HF frequencies are often enhanced by a few dB just before they fade away at dawn. The polarization and/or direction of the arrival of ionospherically propagating signals in the lower HF range (3-5MHz show a consistent variation between pre-midnight, post-midnight, and pre-dawn conditions. As is well known, magnetic storms and substorms dramatically affect ionospheric propagation; data from multiple stations spanning the invariant latitude range 67-79° reveal spatial patterns of propagation characteristics associated with magnetic storms and substorms. For example, in the hours preceding many isolated substorms, favorable propagation conditions occur at progressively lower latitudes as a function of time preceding the substorm onset. For some of these effects, explanations follow readily from elementary ionospheric physics, but understanding others requires further investigation.

    Key words. Magnetospheric physics (annual phenomena – Radio science (ionosphere propagation; radio-wave propagation6

  7. RPW: Very Low Electric Field Measurements for LF Bandwidth (F<20MHZ)

    Science.gov (United States)

    Panh, J.; Fiachetti, C.; Berge, R.; Guilhem, E.; Lourme, E.; Sampl, M.; Kapper, M.

    2016-05-01

    Embedding RPW (Radio Plasma Wave) instrument on Solar Orbiter implies to be able to measure very low level of the electric field from DC to 20MHz. And this low level is required either for RPW antenna calibration or for radiated emission qualification of the other units and instruments of the spacecraft. This document presents the steps in order to define an applicable setup. The first part of this document presents the context of the study and the levels to work with. The second part concerns the characterisations of the performances of the antenna. The last part presents the setups that allow achieving low level and the approach to do an estimation of the field expected in flight.

  8. Investigation of the Effect of Low Level Maritime Haze on DMSP VHR and LF Imagery

    Science.gov (United States)

    1980-12-01

    P.O. BOX 1 736 WASHINGTON, DC 20013 SERVICIO METEOROLOGICO DE LA ARMADA EDIFICIO LIBERTAD , PISO 15 COMODORO PY Y CORBETA URUGUAY (1104) BUENOS...AIRES REPUBLICA ARGENTINA LIBRARIAN METEOROLOGY DEPT. UNIVERSITY OF MELBOURNE PARKVILLE, VICTORIA 3052 AUSTRALIA BUREAU OF METEOROLOGY ATTN

  9. Genetic diversity of teak (Tectona grandis L.F. from different provenances using microsatellite markers

    Directory of Open Access Journals (Sweden)

    Berenice Kussumoto Alcântara

    2013-08-01

    Full Text Available Teak (Tectona grandis is one of the main timber species in the world with high economic value, famous for its beauty, strength and durability. The objective of this work was to characterize the genetic diversity of teak genotypes used in Brazilian plantations. Nine microsatellite primers were used to assess 60 teak genotypes, including 33 genotypes from seeds of plantations and 14 clones from Cáceres municipality, Mato Grosso State, Brazil, and 13 clones from Honduras, Malaysia, India, Indonesia, Ivory Coast and Solomon Islands. Two groups of genotypes were detected using the Bayesian Structure analysis: 80% were placed in group 1, represented by genotypes from Cáceres and one from Malaysia, and 20% allocated in group 2, composed of clones from India, Solomon Islands, Malaysia and Honduras and the clones from the Ivory Coast. Most of the genetic variability (73% was concentrated within groups according to AMOVA analysis. Genetic parameters were estimated for the two groups obtained in the analysis of Structure. Moderate genetic diversity was found, with 4.1 alleles per locus, on average, and an average heterozygosity of 0.329, which was lower than the expected heterozygosity (He = 0.492. Group 1 showed the lowest values for these parameters. Suggestions were made concerning the identification of contrasting genotypes to be used as parents in breeding programs.

  10. Denk je zèlf! Developing a personalised virtual coach for emotional eaters using personas

    NARCIS (Netherlands)

    Dol, A.; Kulyk, O.; Velthuijsen, H.; Gemert-Pijnen, L. van; Strien, T. van

    2016-01-01

    Obesity is a fast growing societal threat, causing chronic conditions, physical and psychological health problems, as well as absenteeism and large healthcare costs. Despite numerous attempts to promote physical activity and healthy diet, existing interventions do not focus on often occurring emotio

  11. FENOLOGÍA FLORAL Y VISITANTES FLORALES EN Drimys granadensis L.f. (WINTERACEAE

    Directory of Open Access Journals (Sweden)

    XAVIER MARQUINEZ

    2009-01-01

    Full Text Available El propósito de este trabajo fue caracterizar las fases fenológicas florales y determinar los visitantes florales en una población natural de Drimys granadensis (nombre común: canelo; Winteraceae ubicada en Altos de Yerbabuena (2.850 m, cerros orientales de la Sabana de Bogotá, (Colombia. El desarrollo fenológico floral duró 9,5 días cuando la floración ocurrió en época soleada, 12,5 días en época lluviosa y 16 días cuando se excluyeron a los visitantes florales mediante embolsado. Se realizaron observaciones del recurso utilizado, fase fenólogica de la flor visitada y cargas polínicas en los visitante florales, los cuales correspondieron a seis órdenes, 21 familias y 29 morfoespecies de insectos. Cuatro especies de coleópteros y dos de dípteros fueron considerados posibles polinizadores por su abundancia y carga de polen. Los resultados obtenidos se discuten en relación con aquellos reportados en otras especies del género Drimys y de la familia Winteraceae.

  12. VLF/LF (Very Low Frequency/Low Frequency) Reflection Properties of the Low Latitude Ionosphere

    Science.gov (United States)

    1988-02-04

    pleasure for the U.S. personnel. Although it is virtually impossible to mention all the individuals who contributed, we wish to especially ... Educacional da Regiao de Blumenau (FURB), who planned the day-to-day experimental efforts and who provided valuable technical Insight and guidance to all...or more grazing incidence angle, with the trend being especially pronounced in the daytime. Figure 23 shows 20 kHz reflectiont coefficients derived

  13. An Overview Discussion of Propagation Effects of Nuclear Environments on VLF-LF Communication Systems

    Science.gov (United States)

    1975-08-31

    ATTN: YSEV, Lt Col David C. Sparks ATTN: John E. DonCarlos ATTN: XRE, Lt Michaels:- Lt Col. J. Morin , CDEF XRC Commander Naval Electronics...ATITN:. Code 7750, Timothy P. Coffey ATTN, MNX ATTN: Code 7720, Edgar A. McClean S cy ATTN: Code 7701, Jack D. Brown SAMSO/SZ ATTN: Code 5460, Radio...Sen ATTN: D. Pledger System Development Corporation ATTN: S. A. Morin ATTN.. E. G. Meyer ATTN: G. Harding Technology International Corporation The Mitre

  14. An overview on preseismic anomalies in LF radio signals revealed in Italy by wavelet analysis

    Directory of Open Access Journals (Sweden)

    A. Ermini

    2008-06-01

    Full Text Available Since 1996, the electric field strength of the two broadcasting stations MCO (f=216 kHz, southeast France and CZE (f=270 kHz, Czech Republic has been sampled every ten minutes by a receiver (AS located in central Italy. Here, we review the results obtained by a detailed analysis applied to the data recorded from February 1996 up to December 2004. At first, the daytime and nighttime data were extracted and then, in the daytime data, the data collected in winter were separated from those collected in summer. On the second step the wavelet transform was applied. The results of this analysis are radio anomalies detected as earthquake precursors both for MCO and CZE data. In particular, regarding the MCO data, the main result was the appearance of a very clear anomaly during May-August 1998, at daytime and at nighttime. Such an anomaly can be considered as a precursor of a seismic sequence started on August 15, 1998 with 17 earthquakes (M=2.2-4.6 on the Reatini mountains, a seismogenic zone located 30 km far from the AS receiver along the path MCO-AS. As concerns with the CZE data, the first result was obtained from the summer daytime data and it was the appearance of a very clear anomaly during August-September 1997, that can be considered a precursor of the two earthquakes with magnitude M=5.6 and M=5.9 that occurred on September 26 in the Umbria-Marche region (Central Italy. The second result was the appearance of an anomaly during February-March 1998, at daytime and at nighttime, that can be related to the preparatory phase of the strong (M=5.1-6.0 Slovenia seismic sequence that occurred in a zone lying in the middle of the CZE-AS path.

  15. A PHARMACOGNOSTIC EVALUATION ON MOORVA BHEDA (DREGEA VOLUBILIS (L.F. BENTH. EX HOOK.F.

    Directory of Open Access Journals (Sweden)

    Karthika K S

    2012-09-01

    Full Text Available Herbal medicines are traditionally used for treatment of various illnesses. Hence, medicinal plants have been receiving great attention worldwide by the researchers because of their safe utility. The plant Dregea volubilis (syn. Wattakaka volubilis, Marsdenia volubilis is considered as a source plant of the Ayurvedic drug Moorva, is a climbing shrub of the family Apocynaceae. Traditionally, the plant is useful in different conditions such as pain, cold, boils, abscesses etc. In different states of India it is used by folks as a successful remedy in eye diseases. The study comprises includes macroscopy and microscopic of leaf, petiole, stem and root of D. volubilis and powder microscopy of leaf powder. This helps in correct botanical identification and characterization of the drug Dregea volubilis. Study reveals, in leaf numerous prismatic, rosettes and cluster crystals, lower epidermis had two types of stomata (paracytic and anomocytic, but stomata was absent in upper epidermis. Stem contain patches of non lignified fibers in cortex and presence of rosettes and cluster crystals, where as root had bands of stones cells in the cortex.

  16. GuLF Study: The Gulf Long-Term Follow-Up Study

    Science.gov (United States)

    ... Studies Study Publications Mexico Childhood Asthma Studies Mexico Childhood Asthma Study Publications MOBAND Study of Cerebral Palsy Studies MOBAND Study of Cerebral Palsy Publications North Carolina Infant Feeding Study Studies North Carolina Infant Feeding Study Study ...

  17. A singing voices synthesis system to characterize vocal registers using ARX-LF model

    OpenAIRE

    Motoda, Hiroki; Akagi, Masato

    2013-01-01

    This paper proposes a singing voices synthesis system to synthesize singing voices having characteristics of vocal registers, such as vocal fly, modal and falsetto. Human can sing songs naturally in wide range of frequency by training how to use vocal fold vibrations to represent vocal registers. However, even state-of-the-art singing voices synthesis systems cannot produce vocal registers appropriately. Naturalness of the synthesized singing voices using these systems is reduced in low and h...

  18. Active implantable medical device EMI assessment for wireless power transfer operating in LF and HF bands

    Science.gov (United States)

    Hikage, Takashi; Nojima, Toshio; Fujimoto, Hiroshi

    2016-06-01

    The electromagnetic interference (EMI) imposed on active implantable medical devices by wireless power transfer systems (WPTSs) is discussed based upon results of in vitro experiments. The purpose of this study is to present comprehensive EMI test results gathered from implantable-cardiac pacemakers and implantable cardioverter defibrillators exposed to the electromagnetic field generated by several WPTSs operating in low-frequency (70 kHz-460 kHz) and high-frequency (6.78 MHz) bands. The constructed in vitro experimental test system based upon an Irnich’s flat torso phantom was applied. EMI test experiments are conducted on 14 types of WPTSs including Qi-compliant system and EV-charging WPT system mounted on current production EVs. In addition, a numerical simulation model for active implantable medical device (AIMD) EMI estimation based on the experimental test system is newly proposed. The experimental results demonstrate the risk of WPTSs emitting intermittent signal to affect the correct behavior of AIMDs when operating at very short distances. The proposed numerical simulation model is applicable to obtain basically the EMI characteristics of various types of WPTSs.

  19. Effects of a Solar Eclipse on the Propagation of VLF-LF Signals: Observations and Results

    Directory of Open Access Journals (Sweden)

    Barin Kumar De

    2011-01-01

    Full Text Available The results from the measurements of some of the fundamental parameters (amplitude of sferics and transmitted signal, conductivity of lower ionosphere of the ionospheric responses to the 22 July 2009 solar eclipse (partial: 91.7% are shown. This study summarizes our results from sferics signals at 81 kHz and subionospheric transmitted signals at 19.8 and 40 kHz recorded at Agartala, Tripura (latitude: 23¢XN, longitude: 91.4¢XE. We observed significant absorption in amplitude of these signals during the eclipse period compared to their ambient values for the same period during the adjacent 7 days. The signal strength along their propagation paths was controlled by the eclipse associated decrease in ionization in the D-region of the ionosphere. Waveguide mode theory calculations show that the elevation of the height of lower ionosphere boundary of the Earth-ionosphere waveguide to a value where the conductivity parameter was 106 unit. The absorption in 81 kHz sferics amplitude is high compared to the absorption in the amplitude of 40 kHz signal transmitted from Japan. The simultaneous changes in the amplitudes of sferics and in the amplitude of transmitted signals assert some sort of coupling between the upper atmosphere and the Earth¡¦s near-surface atmosphere prevailing clouds during solar eclipse.

  20. End-pumped all solid-state high repetition rate Tm, Ho:LuLF laser

    Institute of Scientific and Technical Information of China (English)

    Shijiang Shu; Ting Yu; Junyan Hou; Rongtao Liu; Minjie Huang; Weibiao Chen

    2011-01-01

    @@ The characteristics of diode end-pumped Tm,Ho:LuLiF for continuous wave (CW) running and high pulse repetition frequency (PRF) Q-switched operation are illustrated. In the CW mode, 950-mW output power with a slope efficiency of 24% is obtained. In the Q-switched mode, output energy of 78 μJ under 10 kHz with a slope efficiency of 23% is achieved. The pulse stability, pulse width as a function of pump intensity, and spectral characteristics are also analyzed.%The characteristics of diode end-pumped Tm,Ho:LuLiF for continuous wave (CW) running and high pulse repetition frequency (PRF) Q-switched operation are illustrated. In the CW mode, 950-mW output power with a slope efficiency of 24% is obtained. In the Q-switched mode, output energy of 78μJ under 10 kHz with a slope efficiency of 23% is achieved. The pulse stability, pulse width as a function of pump intensity, and spectral characteristics are also analyzed.

  1. lfärdsbilder : svensk film utanför biografen

    OpenAIRE

    2008-01-01

    In this anthology, twenty-three scholars from seven academic disciplines analyze film in Sweden never distributed to the cinema. Encompassing more than a centurys, the book gives a broad overview of the role of moving images in relation to the the Swedish model welfare state. The material includes amateur film, education film, industrial film and avant-garde film. The book studies these and other film types frpm five interrelated perspectives: early, institutional, art, private, and contempor...

  2. Missile Plume Temperature Sensor and ARCAS Rocket VLF/LF Payload

    Science.gov (United States)

    1975-08-01

    would be met. W,- A test run of the system was made using internal battery power and a dummy load in place of the antenna. The system operated for...also discovered that standard solid state transmitters for the " sonde " class of experiments were not readily available as off-the-shelf items. Most of...of the RCA models or the VIZ sonde transmitter. The VIZ unit is reputed to be quite reliable and provides more than ade- quate output power (300 mw

  3. Optimization of DNA extraction for ISSR studies in Tectona grandis Lf

    African Journals Online (AJOL)

    GRACE

    2006-07-03

    Jul 3, 2006 ... INTRODUCTION. Molecular marker studies require large amount of quality ... and high-density genome mapping (Godwin et al., 1997). MATERIAL .... high metabolic turnover requiring de novo synthesis of transcripts (RNA).

  4. Preliminary phytochemical screening and evaluation of antibacterial activity of Dichrocepala integrifolia (L.f O. kuntze

    Directory of Open Access Journals (Sweden)

    Tesfaye Mohammed

    2012-02-01

    Conclusion and Recommendation: In general the antimicrobial activity of the plant observed here support the tradition therapeutic claim of the society. Further study should be conducted in further evaluating its antimicrobial effectiveness and also purification of the active chemical constituents that could be responsible for its biological activity. [J Intercult Ethnopharmacol 2012; 1(1.000: 30-34

  5. On the Projective Description of Weighted (LF-Spaces of Continuous Functions

    Directory of Open Access Journals (Sweden)

    Catherine V. Komarchuk

    2014-01-01

    Full Text Available We solve the problem of the topological or algebraic description of countable inductive limits of weighted Fréchet spaces of continuous functions on a cone. This problem is investigated for two families of weights defined by positively homogeneous functions. Weights of this form play the important role in Fourier analysis.

  6. U.S. Department of Agriculture/Corps of Engineers Cooperative Aquatic Plant Control Research. Annual Report for FY 1981. Biological and Chemical Control Technologies.

    Science.gov (United States)

    1983-10-01

    aed" the Melt s lraefed voraciously on alligatorweed 16 Mandalay , Burma (14-15 July) In the Mandalay -Sagaing area I searched for hydrilla and...hydrilla is not present in flooded areas. There are patches of hydrilla in the moat surrounding Mandalay Fort. This hydrilla also shows little...pressure. The most common aquatic plant in Mandalay Moat is coontail, Ceratophyllum sp. Bangkok, Thailand (16-22 July) In Bangkok I discussed project goals

  7. Effects of submerged plants on growth performance and non-specific immunity of Chinese mitten crab (Eriocheir sinensis)%沉水植物对中华绒螯蟹生长和非特异性免疫力的影响

    Institute of Scientific and Technical Information of China (English)

    孙丽萍; 宋学宏; 朱金荣; 孟祥雨; 张磊磊

    2012-01-01

    从东太湖采集伊乐藻(Elodea canadensis)、金鱼藻(Ceratophyllum demersum)、轮叶黑藻(Hydrilla verticillata)、苦草(Vallisneria natans)、马来眼子菜(Potamogeton malaianus)5种沉水植物分别制成干粉.在基础饲料中添加15%的干粉形成5种不同的配合饲料,分别饲喂体重为(9.37±0.15)g的中华绒螯蟹(Eriocheir sinensis)60 d,分析沉水植物对中华绒螯蟹生长和非特异性免疫力的影响.结果显示:马来眼子菜组的中华绒螯蟹平均增重率显著低于对照组(P<0.05),饲料系数显著高于对照组(P<0.05),其余4种沉水植物组的平均增重率和饲料系数与对照组无显著差异(P>0.05);各组成活率、肥满度及肝体比差异不显著(P>0.05).沉水植物各组的蟹血清超氧化物歧化酶活力显著高于对照组(P<0.05);与对照组相比,轮叶黑藻组、伊乐藻组和苦草组的中华绒螯蟹血清溶菌酶活性显著升高(P<0.05),金鱼藻组和马来眼子菜组则差异不显著(P>0.05);除马来眼子菜组降低外,其余4种沉水植物组中华绒螯蟹对嗜水气单胞菌的抵抗力强于对照组.因此,除马来眼子菜以外的4种沉水植物对中华绒螯蟹的生长性能无明显的促进作用,但可在一定程度上提高其非特异性免疫力.%The five submerged plants, Elodea canadensis, Ceratophyllum demersum, Hydrilla verticillata, Vallisneria na-tans and Potamogeton malaianus, were collected in East Lake Taihu, and made into dry powders. The dry powders were then incorporated into a basal diet by 15% , respectively, to obtain 5 different mixed diets, and were used to feed Chinese mitten crab ( Eriocheir sinensis ) weighing ( 9. 37 ± 0. 15 ) g for 60 days. The effects of these submerged plants on growth performance and non-specific immunity of Chinese mitten crab were examined. The results showed that the average weight gain rate ( WGR) of Chinese mitten crab in P. Malaianus fed group was significantly lower than

  8. Expanded Simulation Models "Version 3.0" for Growth of the Submerged Aquatic Plants American Wildcelery, Sago Pondweed, Hydrilla, and Eurasian Watermilfoil

    Science.gov (United States)

    2007-11-01

    descriptions of the vegetation responses to daily changes in current velocity and epiphyte shading, and accommodation of daily changes in water level...in current velocity, and epiphyte shading, or to combinations of factors. Once the vegetation is lost from a given locale, increased sediment...responses to changes in current velocity and light attenuation by epiphytes and allow only annual changes in water level. These versions are available

  9. Biomonitoring of selected freshwater macrophytes to assess lake trace element contamination: a case study of Nal Sarovar Bird Sanctuary, Gujarat, India

    Directory of Open Access Journals (Sweden)

    Rita N. KUMAR

    2006-02-01

    Full Text Available A biomonitoring study was carried out at Nal Sarovar Bird Sanctuary, a proposed Ramsar site, Gujarat State, India, to ascertain the degree of trace element contamination. The study focused on assessment of trace element contamination in certain aquatic macrophytes to be used as biomonitors, in comparison with the sediments (abiotic monitor for heavy metal pollution. Good information was provided by analyzing roots, stems and leaves of native aquatic plants (biomonitors represented by eight species: Bergia odorata, Hydrilla verticillata, Ipomoea aquatica, Najas graminea, Nelumbo nucifera, Phragmites karka, Typha angustata and Vellisnaria spiralis, alongwith surface sediments and water, were analyzed for Cd, Co, Cu, Cu, Ni, Pb and Zn contamination. The highest concentrations of the trace elements were measured in Ipomoea aquatica and the lowest in Bergia odorata. Based on the concentration and toxicity status observed in the lake's vegetation, the six metals are arranged in the following decreasing order: Zn > Cu > Ni > Co > Pb > Cd. Compared with the standard, normal and critical toxicity range in plants, the detected values of Cd and Pb falls within normal range, while that of Co, Ni and Cu were within the critical range. However, Zn showed the highest concentration and alarming toxicity levels, which is considered as one of the most hazardous pollutants in Nal Sarovar Bird Sanctuary. Certain aquatic macrophytes species are also proposed as biomonitors for the investigated heavy metal pollutants. Such result was significant in the plant species such as Ipomoea aquatica and Phragmites karka, which are the two most useful species in biomonitoring studies due to their ability to accumulate elements in high concentration in the roots and their availability throughout the year. The results showed the significant difference in accumulation rate of some metals like Zn, Cu and Ni in different plant organs, which showed more accumulation in root than

  10. Bioavailable phosphorus (P) reduction is less than mobile P immobilization in lake sediment for eutrophication control by inactivating agents.

    Science.gov (United States)

    Wang, Changhui; He, Rui; Wu, Yu; Lürling, Miquel; Cai, Haiyuan; Jiang, He-Long; Liu, Xin

    2017-02-01

    Phosphorus (P) immobilization by inactivating agents in the sediment of eutrophic lakes to reduce immediately available P in lake water is often crucial for mitigating nuisance eutrophication symptoms, such as cyanobacterial blooms. Macrophytes and phytoplankton, however, can directly utilize P from the sediment for growth. Accordingly, a comprehensive analysis of the P bioavailability in lake sediment amended with two promising P-inactivation agents, namely Phoslock(®) and drinking water treatment residue (DWTR), was investigated in both short- and long-term studies (20 and 180 d). Phosphorus-availability was assessed using six chemical extraction methods and Hydrilla verticillata and Microcystis aeruginosa growth tests. The results showed that Phoslock(®) and DWTR significantly reduced mobile P (NH4Cl and Na2S2O4/NaHCO3 extractable P) in lake sediment, while P bioavailability that was assessed by different methods showed considerable deviations. Interestingly, appropriate bioavailable P chemical extraction methods were determined based on linear correlation analysis, and further comparison indicated that reduction of bioavailable P by DWTR (P) and Phoslock(®) (P) were clearly less than the mobile P immobilization (>75%) at recommended dosages, which was probably caused by the capability of macrophyte and cyanobacteria to utilize various fractions of P (except the residual P) in amended sediment under proper illumination. Therefore, DWTR and Phoslock(®) can effectively reduce P release from lake sediment, but the potential bioavailable P may pose uncertainties for eutrophication control in lakes that typically have regular sediment re-suspension. Overall, an evaluation of the bioavailable P pool in the lake ecosystem should be essential for successful lake geo-engineering.

  11. Cultivation of macroscopic marine algae and freshwater aquatic weeds. Progress report, May 1-December 31, 1978

    Energy Technology Data Exchange (ETDEWEB)

    Ryther, J. H.

    1979-01-01

    Studies were continued during 1977 to 1978 on the growth and yields in culture of the red seaweed Gracilaria tikvahiae. Partial control of epiphytes was achieved by nutrient removal, shading, and/or biological agents. For the first time, a single clone of the alga was grown continuously throughout the year without replacement. Yields in large (2600 1) aluminum tanks averaged 21.4 g dry weight/m/sup 2/.day, equivalent to 31 tons/acre.year (15.5 ash-free dry wt tons/acre.year). Growth of Gracilaria and other seaweeds in Vexar-mesh baskets in natural habitats and in the oceanic waters of a power plant cooling water intake canal were unsuccessful. Productivity of the freshwater macrophytes Lemna minor (common duckweed), Eichhornia crassipes (water hyacinth), and Hydrilla verticillata have now been measured throughout the year with mean yields of 3.7, 24.2 and 4.2 g dry weight/m/sup 2/.day (5.4, 35.3, and 6.1 dry tons/acre.year) respectively. Yields of duckweed and water hyacinths in the Harbor Branch Foundation culture units have averaged roughly three times those of the same species growing in highly-eutrophic natural environments. The yields of several other species of freshwater plants were investigated. Only the pennywort (Hydrocotyle umbellata) appears to approach the productivity of water hyacinth on the basis of preliminary measurements. Chopped water hyacinths and unprocessed Gracilaria have both been successfully fermented to methane in anaerobic digesters and the liquid digester residues recycled to produce more of the same plants.

  12. Assessment of Wetland Water Quality and Avian Diversity of a Human-Modified Floodplain Wetland on River Yamuna

    Directory of Open Access Journals (Sweden)

    Upma MANRAL

    2013-02-01

    Full Text Available Okhla Bird Sanctuary (OBS is an Important Bird Area, which comes under the protected area network of Uttar Pradesh with one-third area lying in the state of Delhi (India. OBS has widest flood plains along the Delhi stretch of river Yamuna and is important in conserving the ecological wealth of floodplains of the river. Rapid urbanization and industrialization and discharge of untreated wastewater into the river have resulted in deteriorated water quality. The present study focused on assessment of water quality, aquatic flora and avifaunal diversity in the OBS. Water quality was analyzed following methods of APHA. For vegetation analysis, sub-merged and free-floating plants were scooped up from five randomly selected sites. Total bird counts were conducted for water birds and species richness, evenness and Shannon-Weaver species diversity indices were calculated. Results indicate that the organic load is very high in the wetland as evident from low levels of dissolved oxygen (2.26 ± 1.62 mg/l and high Biological and Chemical Oxygen Demands (15.20 ± 3.75 mg/l, 44.60 ± 12.07 mg/l. Nine species of free-floating and submerged plants were recorded; Hydrilla verticillata, Vallisneria spiralis, Azolla pinnata and Ceratophyllum demersum dominated both deep and shallow water areas. 52 species of waterbirds including four near-threatened species viz., Anhinga melanogaster, Mycteria leucocephala, Threskiornis melanocephalus and Aythya nyroca were recorded. OBS provides opportunities for conservation in a metropolitan area, thus, appropriate measures should be taken to maintain its ecological integrity.

  13. Influences of chemical components of water extracts from submerged macrophyte leaves on the growth of three common epiphytic algae%沉水植物浸提液组分对三种常见附植藻类生长的影响

    Institute of Scientific and Technical Information of China (English)

    纪海婷; 谢冬; 周恒杰; 安树青

    2015-01-01

    采用长江中下游湖泊典型沉水植物乡土种轮叶黑藻(Hydrilla verticillata)、穗状狐尾藻(Myriophyllum spicatum)和外来种伊乐藻(Elodea nuttallii)、水盾草(Cabomba caroliniana)叶片浸提液的组分:N-苯基-2-萘胺(4种沉水植物均有)、苯丙酮(伊乐藻特有)和苯并呋喃(水盾草特有),研究不同物质种类及浓度对3种附植藻类:普通小球藻(Chlorella vulgaris)、斜生栅藻(Scenedesmus obliqnus)和四尾栅藻(S.quadricanda)的相对生长率、藻细胞浓度、叶绿素含量等生长指标的影响.结果表明:3种物质均显著降低斜生栅藻和普通小球藻的细胞浓度以及叶绿素含量,对四尾栅藻生长的抑制较弱.3种附植藻类生长指标对不同物质浓度及处理时间的响应存在显著差异,呈现低浓度促进、高浓度抑制现象,随时间推移藻类显示出明显的生长趋势.物质作用效果:苯丙酮>苯并呋喃>N-苯基-2-萘胺,表明外来种沉水植物叶片浸提液中特有物质具有较强的抑藻效应.

  14. Effects of leaf extracts from two kinds of submerged macrophytes on the growth and community composition of epiphytic algae living on native macrophyte species%两类沉水植物浸提液对乡土种叶附植藻类生长与群落组成的影响

    Institute of Scientific and Technical Information of China (English)

    纪海婷; 谢冬; 周恒杰; 安树青

    2013-01-01

    将外来种沉水植物伊乐藻(Elodea nuttallii)、聚叶狐尾藻(Myriophyllum aquaticum)分别与其同科(或同属)的乡土种沉水植物轮叶黑藻(Hydrilla verticillata)、穗状狐尾藻(Myriophyllum spicatum)进行对比,分析了两种乡土沉水植物叶表面附植藻类的群落组成与结构特征,采用植物浸提液法研究不同浓度(0.5和2 mg·mL-1)的叶片浸提液对乡土种叶附植藻类生长和群落组成的影响.结果表明:两种外来沉水植物较乡土种具有更强的抑藻效应,且在高浓度下更显著;4种沉水植物浸提液均降低乡土种叶附植藻类的细胞总数,对群落物种丰富度和多样性的影响总体呈“低浓度促进、高浓度抑制”,且外来种的抑制更显著,其浸提液使得附植藻类群落组成结构趋于简单化;植物鲜样制备的浸提液较植物干样制备的浸提液具有更强的化感抑藻作用.

  15. The effects of Bellamya sp. on the growth of two submerged macrophytes in Lake Taihu%太湖环棱螺对两种常见沉水植物生长的影响

    Institute of Scientific and Technical Information of China (English)

    白秀玲; 谷孝鸿; 张钰

    2007-01-01

    环棱螺(Bellamya sp.)是太湖常见的一类软体动物.本研究通过室内实验,探讨环棱螺对轮叶黑藻(Hydrilla verticillata Royle)和伊乐藻(Elodea nuttalli ST John)生长的影响及水体营养盐含量的变化.结果表明,三种处理情况下,单位质量伊乐藻增加的数量分别为:H组0.475 g,L组0.106 g,C组0.021 g,单位质量轮叶黑藻增加的数量分别为:H组0.704 g,L组0.663 g,C组0.478 g.从实验前后两种沉水植物的长度和分蘖数变化来看,H组最高,L组与C组分别次之,所以不论生物量、长度还是分蘖数的变化量,与环棱螺共存的伊乐藻和轮叶黑藻的变化都高于对照组中两种沉水植物的变化.环棱螺新陈代谢促进水体中溶解态氮磷含量增加,三种情况下水生植物的初级生产力都相当,由此可推测环棱螺通过新陈代谢,一定程度上促进了两种沉水植物的生长.

  16. Influence of temperature on the growth and nutrient removal of five submerged macrophytes%温度对五种沉水植物生长和营养去除效果的影响

    Institute of Scientific and Technical Information of China (English)

    闫志强; 刘黾; 吴小业; 刘燕; 潘美婷; 王嘉华; 蔡丽桃; 李秋宇; 陈章和

    2014-01-01

    研究温度对黑藻(Hydrilla verticillata)、伊乐藻(Elodea canadensis)、狐尾藻(Myriophyllum spicatum)、苦草(Vallisneria spiralis)和竹叶眼子菜(Potamogeton malaianus)在霍格兰氏水培条件下的生长和营养去除效果的影响.结果表明:温度对五种沉水植物的生长有显著影响.除苦草的最适生长温度为30℃外,其余四种植物的最适生长温度为20℃.五种植物的生长速度有显著性差异,以伊乐藻、竹叶眼子菜、黑藻生长较快.对TP和CODcr的去除率随温度的升高而升高,对NH4-N的去除率除竹叶眼子菜外,呈单峰曲线,去除率最适温度为25℃.五种沉水植物对TP的去除率通常差异不显著;竹叶眼子菜对NH4-N去除率显著低于其他植物(p<0.05),而对CODcr的去除率显著高于其他植物(p<0.05).

  17. Cultivation of macroscopic marine algae and freshwater aquatic weeds. Progress report, May 1, 1978-September 30, 1979

    Energy Technology Data Exchange (ETDEWEB)

    Ryther, J.H.

    1980-04-01

    Studies were continued during 1977-1978 on the growth and yields in culture of the red seaweed Gracilaria tikvahiae. Partial control of epiphytes was achieved by nutrient removal, shading, and/or biological agents. For the first time, a single clone of the alga was grown continuously throughout the year without replacement. Yields in large (2600 1) aluminum tanks averaged 21.4 g dry weight/m/sup 2/.day, equivalent to 31 tons/acre.year (15.5 ash-free dry wt tons/acre.year). Growth of gracilaria and other seaweeds in Vexar-mesh baskets in natural habitats and in the oceanic waters of a power plant cooling water intake canal were unsuccessful. Productivity of the freshwater macrophytes Lemna minor (common duckweed), Eichhornia crassipes (water hyacinth), and Hydrilla verticillata have now been measured throughout the year with mean yields of 3.7, 24.2 and 4.2 g dry weight/m/sup 2/.day (5.4, 35.3, and 6.1 dry tons/acre.year) respectively. Yields of duckweed and water hyacinths in the Harbor Branch Foundation culture units have averaged roughly three times those of the same species growing in highly-eutrophic natural environments. Chopped water hyacinths and unprocessed Gracilaria have both been successfully fermented to methane in anaerobic digesters and the liquid digester residues recycled to produce more of the same plants. A preliminary budget for recycled nitrogen has been determined for water hyacinths. Productivity of both water hyacinths and Gracilaria has been calculated from nitrate-nitrogen assimilation and good agreement with measured yields was obtained.

  18. Attempts to reproduce vacuolar myelinopathy in domestic swine and chickens.

    Science.gov (United States)

    Lewis-Weis, Lynn A; Gerhold, Richard W; Fischer, John R

    2004-07-01

    Avian vacuolar myelinopathy (AVM) was first recognized as a cause of bald eagle (Haliaeetus leucocephalus) mortality in 1994 in Arkansas (USA) and has since caused over 90 bald eagle and numerous American coot (Fulica americana) mortalities in five southeastern states. The cause of AVM remains undetermined but is suspected to be a biotoxin. Naturally occurring AVM has been limited to wild waterbirds, raptors, and one species of shorebird, and has been reproduced experimentally in red-tailed hawks (Buteo jamaicensis). In this study, chickens and swine were evaluated for susceptibility to vacuolar myelinopathy with the intent of developing animal models for research and to identify specific tissues in affected coots that contain the causative agent. Additionally, submerged, aquatic vegetation, primarily hydrilla (Hydrilla verticillata), and associated material collected from a reservoir during an AVM outbreak was fed to chickens in an effort to reproduce the disease. In two separate experiments, six 4-wk-old leghorn chickens and ten 5-wk-old leghorn chickens were fed coot tissues. In a third experiment, five 3-mo-old domestic swine and one red-tailed hawk, serving as a positive control, were fed coot tissues. In these experiments, treatment animals received tissues (brain, fat, intestinal tract, kidney, liver, and/or muscle) from coots with AVM lesions collected at a lake during an AVM outbreak. Negative control chickens and one pig received tissues from coots without AVM lesions that had been collected at a lake where AVM has never been documented. In a fourth experiment, eight 3-wk-old leghorn chickens were fed aquatic vegetation material. Four chickens received material from the same lake from which coots with AVM lesions were collected for the previous experiments, and four control chickens were fed material from the lake where AVM has never been documented. Blood was collected and physical and neurologic exams were conducted on animals before and once per week

  19. Distribution and mass loss of volatile organic compounds in the surficial aquifer at sites FT03, LF13, and WP14/LF15, Dover Air Force Base, Delaware, November 2000-February 2001

    Science.gov (United States)

    Barbaro, Jeffrey R.; Neupane, Pradumna P.

    2002-01-01

    Ground-water and surface-water sampling was conducted in the natural attenuation study area in the East Management Unit of Dover Air Force Base, Delaware to determine the distributions of volatile organic compounds in the vicinity of four sites?Fire Training Area Three, the Rubble Area Landfill, the Receiver Station Landfill, and the Liquid Waste Disposal Landfill. This work was done by the U.S. Geological Survey, in cooperation with the U.S. Air Force, as part of an ongoing assessment of the effectiveness of natural attenuation at these sites. The specific objectives of the study were to (1) determine the areal and vertical extent of the contaminant plumes and source areas, (2) measure volatile organic compound concentrations in ground-water discharge areas and in surface water under base-flow conditions, (3) evaluate the potential for off-site migration of the mapped plumes, and (4) estimate the amount of mass loss downgradient of the Liquid Waste Disposal and Receiver Station Landfills. A direct-push drill rig and previously installed multi-level piezometers were used to determine the three-dimensional distributions of volatile organic compounds in the 30?60-foot-thick surficial aquifer underlying the natural attenuation study area. A hand -driven mini-piezometer was used to collect ground-water samples in ground-water discharge areas. A total of 319 ground-water and 4 surface-water samples were collected from November 2000 to February 2001 and analyzed for chlorinated solvents and fuel hydrocarbons. The contaminant plumes migrating from Fire Training Area Three and the Rubble Area Landfill are approximately 500 feet and 800 feet, respectively, in length. These plumes consist predominantly of cis-1,2-dichloroethene, a daughter product, indicating that extensive dechlorination of tetrachloroethene and trichloroethene has occurred at these sites. With an approximate length of 2,200 feet, the plume migrating from the Receiver Station and Liquid Waste Disposal Landfills is the largest of the three plumes in the East Management Unit. In this plume, the parent compounds, tetrachloroethene and trichloroethene, as well as cis-1,2-dichloroethene, are present downgradient of the source. Vinyl chloride was not detected in the natural attenuation study area. Vertical water-quality profiles indicate that volatile organic compounds are present mainly in the upper part of the surficial aquifer. Plumes of fuel hydrocarbon constituents were not detected in the natural attenuation study area. Volatile organic compounds were present at concentrations above detection limits in 6 of 14 samples collected from the aquifer underlying the bed of Pipe Elm Branch and the drainage ditch adjacent to Fire Training Area Three, indicating that the plumes migrating from Fire Training Area Three and the Receiver Station and Liquid Waste Disposal Landfills are reaching these ground-water discharge areas. In contrast, sampling results indicated that the plume from the Rubble Area Landfill does not reach these ground-water discharge areas. Trichloroethene was present above detection limits in one of four surface-water samples collected from Pipe Elm Branch and the drainage ditch adjacent to Fire Training Area Three. The trichloroethene concentration is below applicable Delaware Department of Natural Resources and Environmental Control surface-water-quality standards for human health. An assessment of chlorinated-solvent mass loss in the plume migrating from the Receiver Station and Liquid Waste Disposal Landfills indicates that tetrachloroethene and trichloroethene mass loss downgradient of the source is negligible. Cis-1,2-dichloroethene, however, appears to biodegrade by an unidentified reaction in the plume. Plan-view maps of the plume migrating from the Receiver Station and Liquid Waste Disposal Landfills indicate that tetrachloroethene, trichloroethene, and cis-1,2-dichloroethene may migrate off Dover Air Force Base property approximately 1,500 f

  20. On-line programming technology of on-chip Flash in TMS320LF240x DSP based on CH376%基于 CH376实现 TMS320 LF240 x片内Flash在线编程

    Institute of Scientific and Technical Information of China (English)

    熊金

    2014-01-01

    This paper particular introduced the software and hardware design that apply with on-line programming technology.The flows of search files and read or write data through CH376, also the con-version method of bin files and on-line programming were provided.This technology applied to practical engineering and brought more convenience and reduced the product recall because of the system update and maintenance.%详细介绍利用CH376芯片实现在线编程的软硬件设计,给出通过CH376实现文件查找和文件数据块读取的详细流程,bin文件的转换方法以及在线编程的流程。该设计成功应用于系统开发中,给系统升级维护提供了诸多便利,减少因系统升级维护导致的产品召回。

  1. 3种沉水植物对夏季高温强光照环境的生理响应%Physiological Response of Three Submerged Macrophytes to the High Temperature and Light Intensity of Summer

    Institute of Scientific and Technical Information of China (English)

    王亚林; 高园园; 于丹; 刘春花

    2015-01-01

    Invasion by exotic species has become a global problem,adversely affecting the environment,economy and even human health in the areas invaded.Elodea nuttallii and Egeria densa,belonging to Hydrocharitaceae,are two exotic submerged macrophytes species in China that display a strong invasive tendency.It has been reported that E.nuttallii and E.densa are both adapted to low temperature and light intensity.Thus,we investigated the physiological response of three Hydrocharitaceae species to the high temperature and light intensity of summer,in-cluding the two exotic species (E.nuttallii and E.densa)and one native species (Hydrilla verticillata).The stud-y was designed to explore the effects of high temperature and light intensity on the invasiveness of the two exotic macrophytes.In August 2014,all test plants were placed in the natural summer condition of high temperature and light intensity,which provided the experimental treatment.The physiological response was measured by determining photosynthetic (total chlorophyll content and the maximum quantum yield of PSII (Fv/Fm))and antioxidant (ma-londialdehyde and proline content)parameters.The physiological traits were measured on day 0,3,6,9,12,15 of the treatment.Results show that high temperature and light intensity significantly affected the physiology of all three species,especially photosynthesis.Furthermore,long term high temperature and light intensity increased the impact.From day 1 to day 3,when the temperature rose to 31℃,the total chlorophyll content of E.nuttallii and the Fv/Fm of the three macrophytes were significantly lower than their initial levels (P <0.05).From day 4 to day 6,the temperature remained at 32℃ and the total chlorophyll content and the Fv/Fm of the three macrophytes were significantly lower than their initial levels (P <0.05).Moreover,the MDA content of E.nuttallii and E.densa was significantly higher than the initial level (P <0.05).During the entire experiment,the total chlorophyll con

  2. An antimicrobial alkaloid and other metabolites produced by Penicillium sp. An endophytic fungus isolated from Mauritia flexuosa L.f

    Energy Technology Data Exchange (ETDEWEB)

    Koolen, Hector Henrique Ferreira; Soares, Elzalina Ribeiro; Silva, Felipe Moura Araujo da; Almeida, Richardson Alves de; Souza, Afonso Duarte Leao de, E-mail: hectorkoolen@gmail.com [Departamento de Quimica, Universidade Federal do Amazonas, Manaus - AM (Brazil); Medeiros, Livia Soman de; Rodrigues Filho, Edson [Departamento de Quimica, Universidade Federal de Sao Carlos, Sao Carlos - SP (Brazil); Souza, Antonia Queiroz Lima de [Escola Superior de Ciencias da Saude, Universidade do Estado do Amazonas, Manaus - AM (Brazil)

    2012-07-01

    The alkaloid glandicoline B (1) and six other compounds: ergosterol (2), brassicasterol (3), ergosterol peroxide (4), cerevisterol (5), mannitol (6) and 1-O-{alpha}-D-glucopyranoside (7) were isolated from Penicillium sp. strain PBR.2.2.2, a fungus from Mauritia flexuosa roots. The structures of the isolated metabolites were established by spectral analysis. MeOH extract of the fungal mycelium at 500 {mu}g mL{sup -1} exhibited antimicrobial activity against Staphylococcus aureus and the compound 1 at 100 {mu}g mL{sup -1} was active against S. aureus, Micrococcus luteus and Escherichia coli. The relationship between the bioactive properties of the fungus PBR.2.2.2 and those achieved for glandicoline B, as well the potential of this substance as bacteriide is discussed. (author)

  3. Investigation of correlation of LF power modulation of light in natural and artificial illumination situations and acoustic emission

    Science.gov (United States)

    Kleeberg, Florian P.; Gutzmann, Holger L.; Weyer, Cornelia; Weiß, Jürgen; Dörfler, Joachim; Hahlweg, Cornelius F.

    2014-09-01

    The present paper is a follow up of a paper presented in 2013 at the Novel Optical Systems conference in the session on Optics and Music. It is derived from an ongoing study on the human perception of combined optical and acoustical periodical stimuli. Originating from problems concerning artificial illumination and certain machinery with coherent optical and acoustical emissions there are effects to be observed which are interesting in the context of occupational medicine. It seems, that acoustic stimuli in the frequency range of the flicker fusion and below might lead to unexpected perceptible effects beyond those of the single stimuli. The effect of infrasound stimuli as a whole body perception seems to be boosted. Because of the difficulties in evaluation of physical and psychological effects of such coherent stimuli in a first step we question if such coherence is perceivable at all. Further, the problem of modulation of optical signals by acoustical signal is concerned. A catalogue of scenarios and 'effects to look for' including measurement concepts is presented and discussed.

  4. Alva Myrdal och svenskt familjeplaneringsbistånd : Affinitet mellan välfärdsideologi och principerna bakom befolkningskontroll

    OpenAIRE

    Lundberg, Simon

    2015-01-01

    Around the 1950s an explosive population growth started in the Third World. Parallel with the population growth a global network grew up advocating population control and limited population growth. Sweden had a unique place in that network through a pioneering role promoting the issue in the UN organization. During the 20th century Sweden was a pioneer country in terms of initiatives for population control. Ceylon family planing pilot project was something new in a time when other donors cons...

  5. ISSR markers for analysis of molecular diversity and genetic structure of Indian teak (Tectona grandis L.f. populations

    Directory of Open Access Journals (Sweden)

    Shamin Akhtar Ansari

    2012-06-01

    Full Text Available Inter simple sequence repeats (ISSR constitute a powerful dominant DNA molecular marker system used for diversity analysis, which is indispensable for making estimates of genetic base and demarcation of populations for undertaking conservation and improvement program offorest tree species. Twenty nine populations of teak (Tectona grandis L.f. were collected from central and peninsular India for analysis of genetic diversity and structure. Genomic DNA from ten randomly selected individuals of each population was extracted and amplified using five ISSR primers (UBC-801, 834, 880, 899 and 900. The primers showed 100% polymorphism. UBC-900 recorded the highest Nei's genetic diversity (0.32 to 0.40 and UBC-899 had the highest Shannon's Information Index (0.49 to 0.59. AMOVA revealed a very high intra-population genetic diversity (91%, in comparison to inter-population genetic diversity among states (6.17% and within states (2.77%, were also indirectly confirmed by large standard deviations associated with genetic diversity estimates for individual population, as well as poor bootstrapping values for most of the cluster nodes. However, UPGMA dendrogram revealed several clusters, with populations from central India being present almost in each cluster, making groups with populations of adjoining states and distant states. Nevertheless, the cluster analysis distinguished the drier teak populations of central India from the moist teak populations of south India, which was also confirmed by Principle Coordinate Analysis. The findings advocates the need not only for enhancing selection intensity for large number of plus trees, but also for laying out more number of in situ conservation plots within natural populations of each cluster for germplasm conservation of teak aimed at improving the teak productivity and quality in future. 

  6. Teak (Tectona grandis L.f.): A preferred timber for shipbuilding in India as evidenced from shipwrecks

    Digital Repository Service at National Institute of Oceanography (India)

    Tripati, S.; Shukla, S.R.; Shashikala, S.; Sardar, A.A.

    anatomy. INDIA has contributed significantly to world culture in the field of science, technology, culture and philosophy. One of the greatest achievements of man was the invention of watercraft made of reeds, logs and skins, which were used from...

  7. Micropropagation ofTigridia pavonia (L.f) DC-a potential floricultural plant from twin scale explants

    Institute of Scientific and Technical Information of China (English)

    Lekha Kumar; Sincy Joseph; Narmatha Bai

    2012-01-01

    Objective:The present study was performed to standardize an effective protocol for micropropagation ofTigridia pavonia using tissue culture.Methods: The explants were cultured on Murashige and Skoog (MS) medium supplemented with cytokinins like thidiazuron (TDZ), zeatin, kinetin and auxins such as indole-3-acetic acid (IAA), 1-naphthalene acetic acid (NAA) and indole-3-butyric acid (IBA), individually at different concentrations.Results:Multiple shoots were obtained on MS medium containing either 2.0 mg/L TDZ or 2.0 mg/L IAA or 0.5 mg/L IBA and in the same medium for a long period (120 d) produced tiny bulbs at the base of the senescent leaves. TDZ favored only multiple shoots without roots, whereas IAA or IBA individually or in combination with TDZ produced rooted shoots. Shoots developed on MS medium supplemented with TDZ were rooted on MS medium containing either IBA or NAA at 0.5 mg/L. The plantlets were acclimatized in pots containing garden soil. Regenerated plantlets developed into normal plants. The plants showed 99% survival.Conclusions:The highest number of bulblets obtained in the present study represents an effective alternative to the conventional method.

  8. Zwölf Grundformen des Lehrens eine allgemeine Didaktik auf psychologischer Grundlage, Medien und Inhalte didaktischer Kommunikation, der Lernzyklus

    CERN Document Server

    Aebli, Hans

    2011-01-01

    Hans Aebli verbindet psychologische Erkenntnisse aus der neueren Zeit mit der Erkenntnis seiner Vorbilder: Jean Piaget, John Dewey, Johann Heinrich Pestalozzi; Psychologen und Pädagogen, deren Einsichten allgemein neu diskutiert werden. Das Buch beweist unmittelbar den Wert einer fundierten didaktischen Theorie und es zeigt, wie sie sich in der Praxis des Unterrichtens einsetzen läßt.

  9. 20 years of Tm:Ho:YLF and LuLF Laser Development for Global Winds Measurements

    Science.gov (United States)

    Singh, Upendra N.; Walsh, Brian M.; Yu, Jirong; Petros, Mulugeta; Kavaya, Michael J.; Barnes, Norman P.

    2014-01-01

    NASA Langley Research Center has a long history of developing 2 micron lasers. From fundamental spectroscopy research, theoretical prediction of new materials, laser demonstration and engineering of lidar systems, it has been a very successful program spanning around two decades. This article covers the program development from the early research to the present instrumentation. A brief historical perspective of Tm:Ho work by early researchers is also given.

  10. Aquatic macrophytes in the large, sub-tropical Itaipu Reservoir, Brazil.

    Science.gov (United States)

    Mormul, Roger Paulo; Ferreira, Fernando Alves; Michelan, Thaisa Sala; Carvalho, Priscilla; Silveira, Marcio José; Thomaz, Sidinei Magela

    2010-12-01

    In the last three decades, rapid assessment surveys have become an important approach for measuring aquatic ecosystem biodiversity. These methods can be used to detect anthropogenic impacts and recognize local or global species extinctions. We present a floristic survey of the aquatic macrophytes along the Brazilian margin of the Itaipu Reservoir conducted in 2008 and compare this with a floristic survey conducted ten years earlier. We used ordination analysis to determine whether assemblage composition differed among reservoir arms. Macrophyte species were sampled in each of the 235 sampling stations using a boat, which was positioned inside three places of each macrophyte stand to record species and search for small plants. We also collected submerged plants using a rake with the boat moving at constant velocity for ten minutes. We assigned individual macrophyte species to life form and identified representative species for each life form. A total of 87 macrophyte taxa were identified. The "emergent" life forms contained the highest number of species, followed by "rooted submerged" life forms. The extensive survey of macrophytes undertaken in September 2008 recorded more species than a survey conducted between 1995 and 1998. This could be due to changes in water physico-chemistry, disturbances due to water drawdown and the long period between surveys, which may have allowed natural colonization by other species. Additionally, differences in the classification systems and taxonomic resolution used in the surveys may account for differences in the number of species recorded. Assemblage composition varied among the arms and was affected by underwater radiation (as measured using a Secchi disk) and fetch. Five non-native species were found. Two of these non-native species (Urochloa subquadripara and Hydrilla verticillata) are of special concern because they have a high frequency of occurrence and occupy large marginal areas of the reservoir. Future surveys should be

  11. Removal and tolerance of 4 submerged plants to nitrogen and phosphorus in reclaimed water%4种沉水植物对再生水中氮磷的去除速率和耐受范围

    Institute of Scientific and Technical Information of China (English)

    徐志嫱; 刘维; 苏振铎; 高杨

    2015-01-01

    [目的]研究4种沉水植物对再生水中氮、磷的去除速率和耐受范围,为以再生水作为补水的景观水体沉水植物的选择提供依据.[方法]以野外选取的伊乐藻(Elodea canadensis)、罗氏轮叶黑藻(Hydrilla verticillata)、菹草(Potamogeton crispus)和金鱼藻(Ceratophyllum demersum)4种沉水植物作为供试材料,设置含不同质量浓度TN和TP的再生水,测定有这4种沉水植物的再生水体中TN和TP质量浓度的变化,构建TN和TP质量浓度与培养时间的回归方程,并在回归方程的基础上,研究4种沉水植物对再生水中的氮、磷的去除规律.[结果]在有4种沉水植物的再生水体中,TN和TP质量浓度均随着培养时间的延长呈负指数衰减变化,沉水植物的净化能力不仅与其种类有关,而且与TN和TP初始质量浓度相关.罗氏轮叶黑藻对TN的去除能力最强,金鱼藻最低;伊乐藻对TP的去除能力最强,金鱼藻最小.菹草对氮素的耐受范围较宽,金鱼藻最窄;伊乐藻对磷素的耐受范围最宽,金鱼藻较窄.[结论]当再生水体中TN初始质量浓度为5~15 mg/L、TP初始质量浓度为0.5~1.5 mg/L时,罗氏轮叶黑藻和伊乐藻对氮磷营养盐的去除速率较高,可作为维持和改善再生水景观水体水质的先锋植物.

  12. 5种沉水植物对斜生栅藻的化感作用%Allelopathic effect of five submerged macrophytes on Senedesmus obliquus

    Institute of Scientific and Technical Information of China (English)

    闫志强; 宋本如; 刘黾; 刘燕; 吴小业; 李洁珊; 林燕珍; 冯翊果; 陈章和

    2015-01-01

    为了解不同湿地植物和不同处理方式下化感作用的差异,在实验室条件下,研究5种常见的沉水植物黑藻(Hydrilla verticillata)、伊乐藻(Elodea nuttallii)、狐尾藻(Myriophyllum spicatum)、苦草(Vallisneria natans)和皇冠草(Echinodorus amazonicus)与斜生栅藻(Scenedesmus obliquus)共培养以及用这5种植物的种植水、浸提液培养斜生栅藻时藻细胞数量及叶绿素a浓度的变化.结果显示,5种植物与斜生栅藻共培养时抑藻效果最明显(1.6×108 cellsL-1),其斜生栅藻细胞数量显著低于种植水(6.2×108 cells L-1)和浸提液组(4.5×108 cells L-1) (P< 0.05),但5种植物间的差异不显著;除黑藻外,其他4种植物的浸提液培养斜生栅藻时均表现出对斜生栅藻明显的抑制效果,斜生栅藻的相对生长率显著低于对照组和黑藻组(P<0.05);5种植物的种植水培养斜生栅藻时则表现出相对较弱的抑藻效果,且植物间差异不显著.本研究表明,沉水植物对藻类的化感作用因不同植物和不同处理而有着显著不同.

  13. Ecological Effects of Exotic and Native Aquatic Vegetation

    Science.gov (United States)

    2009-08-01

    ponds (α=0.05). ..............................................72 Figure 58. Odonata (dragonflies and damselflies) counts were highest in hydrilla ponds...Anax Odonata Dragonflies & damselflies Baetidae Callibaetis Ephemeroptera Mayflies Caenidae Caenis Notonectidae Buenoa Belastomatidae...nificantly smaller and in poorer condition in the latter. Numbers of ERDC/EL TR-09-10 74 bluegills were low in hydrilla ponds. Odonata November Pond

  14. Efeito do volume de tubetes e tipos de substratos na qualidade de mudas de cryptomeria japonica (l.f. d. don.

    Directory of Open Access Journals (Sweden)

    Constâncio Bernardo dos Santos

    2000-01-01

    Full Text Available Cryptomeria japonica (L. F. D. Don. is a species of great potential for planting in elevated and cold regions of Brazil. It is remarkable for its fast growth, good adaptation to the climate and soil of Southern Brazil, and it also shows a good response to silvicultural technologies. In addition, it is notable for the quality and diversity of its raw material usage for the industries of pulp and paper, plywood, construction of houses, furniture, boats, ships, etc. The experimental plan was bifactorial in the entirely randomized design with 8 treatments, 5 repetitions with 40 seedlings per treatment. The results indicated that for the production of seedlings of this species, the substrate soil + vermiculite showed the best development of the seedlings in 120 cm3 containers.

  15. Astrocytes require insulin-like growth factor I to protect neurons against oxidative injury [v1; ref status: indexed, http://f1000r.es/2lf

    Directory of Open Access Journals (Sweden)

    Laura Genis

    2014-01-01

    Full Text Available Oxidative stress is a proposed mechanism in brain aging, making the study of its regulatory processes an important aspect of current neurobiological research. In this regard, the role of the aging regulator insulin-like growth factor I (IGF-I in brain responses to oxidative stress remains elusive as both beneficial and detrimental actions have been ascribed to this growth factor. Because astrocytes protect neurons against oxidative injury, we explored whether IGF-I participates in astrocyte neuroprotection and found that blockade of the IGF-I receptor in astrocytes abrogated their rescuing effect on neurons. The protection mediated by IGF-I against oxidative stress (H2O2 in astrocytes is probably needed for these cells to provide adequate neuroprotection. Indeed, in astrocytes but not in neurons, IGF-I helps decrease the pro-oxidant protein thioredoxin-interacting protein 1 and normalizes the levels of reactive oxygen species. Furthermore, IGF-I cooperates with trophic signals produced by astrocytes in response to H2O2 such as stem cell factor (SCF to protect neurons against oxidative insult. After stroke, a condition associated with brain aging where oxidative injury affects peri-infarcted regions, a simultaneous increase in SCF and IGF-I expression was found in the cortex, suggesting that a similar cooperative response takes place in vivo. Cell-specific modulation by IGF-I of brain responses to oxidative stress may contribute in clarifying the role of IGF-I in brain aging.

  16. Tree-ring analysis of teak (Tectona grandis L.F.) in central India and its relationship with rainfall and moisture index

    Indian Academy of Sciences (India)

    Somaru Ram; H P Borgaonkar; A B Sikder

    2008-10-01

    Tree-ring-width index chronologies of teak (Tectona grandis L.F.)from three sites in central India have been studied for their dendroclimatic potential.The existence of good correlation among the three site chronologies indicates the influence of common forcing factor to the tree growth of the region.Tree growth and climate relationship based on correlation analysis revealed the important contribution of moisture index and rainfall rather than the direct in fluence of the temperature on tree growth during different seasons.Significant positive relationship of moisture index and rainfall during the monsoon months as well as on the annual scale with tree-ring width variations over the region indicates the important role of moisture availability at the root zone.The results suggest that the teak tree-ring chronologies can be used as high resolution proxy for past precipitation and moisture level in the environment.

  17. Chemical characterization and oxidative stability of the oils from three morphotypes of Mauritia flexuosa L.f, from the Peruvian Amazon

    Directory of Open Access Journals (Sweden)

    Mancini-Filho, Jorge

    2010-12-01

    Full Text Available Three morphotypes of aguaje Mauritia flexuosa were tested, classified by the color of their mesocarpium: “color”, “shambo” and “amarillo”, collected from different areas near the city of Iquitos, Peru. Also, physical-chemical analyses of the mesocarpium were performed, such as the characterization of fatty acids by gas chromatography, determination of β- carotene y α – tocopherol by high efficiency liquid chromatography system in normal and reverse phase and the determination of oxidation induction time in the Rancimat apparatus. Proximate, mineral and fatty acid analyses were done on the seeds. The aguaje mesocarpium is rich in oleic oil (“amarillo”: 75.63% ± 0.31, β-carotene (“amarillo”: 342.42ug/g ± 0.71 and α- tocopherol (“color”: 685.81mg/L ± 1.04, plus the morphotype “color” has a superior oxidation induction time compared to other morphotypes with 6.91 ± 0.01. The aguaje seed contains significant amounts of ω6 (linoleic oil in “shambo” with 36.04 ± 0.09%. The results indicate that these oils, regardless their classification, contain important chemical compounds that give them a special nutritive value.Se estudiaron tres morfotipos de aguaje Mauritia flexuosa L. f., clasificados por el color del mesocarpio: “color”, “shambo” y “amarillo”, recogidos de localidades cercanas a la ciudad de Iquitos- Perú. Del mismo modo a partir del mesocarpio se realizaron los análisis fisicoquímicos, la caracterización de los ácidos grasos por cromatografía gaseosa, la determinación de β-caroteno y α– tocoferol por cromatografía líquida de alta eficiencia (HPLC fase reversa y normal y la determinación del tiempo de inducción de la oxidación en el equipo de Rancimat. A partir de la semilla se realizaron los análisis centesimales, de minerales y ácidos grasos. Los mesocarpios de aguaje son ricos en ácido oleico (amarillo: 75.63% ± 0.31, β-caroteno (amarillo: 342.42 ug/g ± 0.71 y α- tocoferol (color: 685.81mg/L ± 1.04, y además el morfotipo “color” tiene un tiempo de inducción superior a los otros morfotipos con 6.91 ± 0.01, asimismo en la semilla de aguaje se encuentran cantidades considerables de ω6 (ácido linoleico en “shambo” con 36.04 ± 0.09%. Los resultados obtenidos nos indican que estos aceites independientemente de la clasificación que se le asigne contienen importantes compuestos químicos que le brindan un altor valor nutricional.

  18. A contingent valuation study of buriti ( Mauritia flexuosa L.f. in the main region of production in Brazil: is environmental conservation a collective responsibility?

    Directory of Open Access Journals (Sweden)

    Irlaine R. Vieira

    Full Text Available ABSTRACT The immature leaves of the buriti palm (Mauritia flexuosa are widely harvested in the municipality of Barreirinhas, Maranhão, for the production of handicrafts, which are sold to locals and tourists. The increasing demand for these artisanal goods is stimulating the emergence of an informal market for immature buriti leaves, leading to an intensification of their extraction and resulting in negative effects on local buriti palm populations and the ecosystem. Thus, the objective of the present study was to assess the environmental value of the buriti palm tree based on the maximum willingness to pay (WTP for its conservation, using the contingent valuation method. Among the respondents, 99.74% reported that the palm species should be protected and the majority of them (65.75% agreed to pay for its conservation (annual WTP R$ 179.49 ± 222.05. Multivariate analysis revealed that the WTP was not influenced by the socio-economic profile of the respondents. The main reasons for non-WTP are related to dissatisfaction with the government and the belief that financial contributions would not solve problems of environmental damage. Overall, the evaluated population believes that environmental conservation is not an obligation of the government or that of the population, but is a collective responsibility.

  19. Diversidad genética y filogenia molecular de poblaciones de Mauritia flexuosa L.f. “aguaje” de la Amazonía Peruana

    Directory of Open Access Journals (Sweden)

    Jorge Angulo-Quintanilla

    2014-07-01

    Full Text Available Mauritia flexuosa es una especie vegetal amazónica que forma extensas poblaciones denominadas “aguajales”. Como los pobladores amazónicos emplean varios órganos de M. flexuosa para suplir sus necesidades y con fines comerciales, se está ejerciendo un gran impacto negativo sobre esta especie. A pesar de ello, a la fecha no se conoce la diversidad genética de esta especie en la Amazonía peruana. Consecuentemente, los planes de manejo para la especie serían limitados sin este tipo de información. Por tanto, el objetivo de esta investigación fue determinar la diversidad genética y filogenia molecular de poblaciones de M. flexuosa aledañas a la carretera Iquitos-Nauta. Las hojas se colectaron en seis zonas contiguas a la carretera Iquitos–Nauta. El ADN purificado con protocolos estándares fue amplificado mediante la técnica de ADN Polimórfico Amplificado al Azar (RAPD con dos cebadores aleatorios.  En total se generaron 28 amplicones RAPD (26 polimórficos y 2 monomórficos. Dentro de los aguajales la diversidad genética fue tres veces mayor (75±19 % que la diversidad genética entre las seis poblaciones de M. flexuosa (25±19 %. La diferenciación genética entre las poblaciones varió de 0,0 a 0,6. Los aguajales que se agruparon en clados en el dendrograma por su mayor similitud genética tuvieron proximidad geográfica. La similitud genética entre las poblaciones de M. flexuosa depende de la distancia geográfica, de tal manera que las poblaciones con más similitud genética están más próximas entre sí que las que tienen menos similitud genética.

  20. Diversidad genética y filogenia molecular de poblaciones de Mauritia flexuosa L.f. “aguaje” de la Amazonía Peruana

    Directory of Open Access Journals (Sweden)

    Jorge Angulo Quintanilla

    2014-06-01

    Full Text Available Mauritia flexuosa es una especie vegetal amazónica que forma extensas poblaciones denominadas “aguajales”. Como los pobladores amazónicos emplean varios órganos de M. flexuosa para suplir sus necesidades y con fines comerciales, se está ejerciendo un gran impacto negativo sobre esta especie. A pesar de ello, a la fecha no se conoce la diversidad genética de esta especie en la Amazonía peruana. Consecuentemente, los planes de manejo para la especie serían limitados sin este tipo de información. Por tanto, el objetivo de esta investigación fue determinar la diversidad genética y filogenia molecular de poblaciones de M. flexuosa aledañas a la carretera Iquitos-Nauta. Las hojas se colectaron en seis zonas contiguas a la carretera Iquitos–Nauta. El ADN purificado con protocolos estándares fue amplificado mediante la técnica de ADN Polimórfico Amplificado al Azar (RAPD con dos cebadores aleatorios.  En total se generaron 28 amplicones RAPD (26 polimórficos y 2 monomórficos. Dentro de los aguajales la diversidad genética fue tres veces mayor (75±19 % que la diversidad genética entre las seis poblaciones de M. flexuosa (25±19 %. La diferenciación genética entre las poblaciones varió de 0,0 a 0,6. Los aguajales que se agruparon en clados en el dendrograma por su mayor similitud genética tuvieron proximidad geográfica. La similitud genética entre las poblaciones de M. flexuosa depende de la distancia geográfica, de tal manera que las poblaciones con más similitud genética están más próximas entre sí que las que tienen menos similitud genética.

  1. Productos del mercado artesanal en la ciudad de Leticia (Amazonia colombiana elaborados con especies de bosques de Mauritia flexuosa L.f.

    Directory of Open Access Journals (Sweden)

    Sandra Lorena Franco Arango

    2015-06-01

    Full Text Available Los productos forestales no maderables (PFNMs han tomado fuerza como alternativa para la conservación de ecosistemas y la generación de ingresos económicos en las poblaciones asociadas. Se presenta un mayor interés sobre los productos que provienen de bosques oligárquicos (poco diversos y con alta densidad relativa, especialmente los dominados por palmas, como por ejemplo los bosques de Mauritia flexuosa (aguajales, cananguchales o buritizales. En el mercado artesanal de Leticia (Amazonia colombiana se identificaron 68 productos provenientes de nueve especies de plantas del canaguchal, teniendo las palmas (Astrocaryum chambira, Euterpe precatoria, M. flexuosa, Oenocarpus bataua y Socratea exorrhiza el mayor potencial comercial. Los productos más frecuentes fueron las mochilas de fibras de A. chambira (chambira y los precios de venta más altos correspondieron a las esculturas de Brosimum rubescens (palo de sangre. No existen estadísticas oficiales de este mercado, ni entes del Estado que regulen y orienten la producción y comercialización de los productos que se extraen del bosque. No obstante, los PFNMs representan una alternativa para diversificar las oportunidades productivas de los pobladores de la región.

  2. DEVELOPING CONCEPTUAL FRAMEWORK FOR REVISING LF-LEARNING MATERIALS (SLMs OF THE OPEN SCHOOL (OS OF BANGLADESH OPEN UNIVERSITY (BOU AT A DIGITAL ENVIRONMENT

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    Sabina YEASMIN

    2011-10-01

    Full Text Available uses the first generation self-learning materials (SLMs written, before an era, following an in-house style and template. The concerned faculty member corrects, every year, texts before the reprint; but this is limited to spelling mistakes, factual errors and page make-ups only. The University has taken policy and steps to revise the texts as a whole which is still limited to the previous process. But; the current government is implementing the agenda of digital Bangladesh which definitely will influence the texts vis-à-vis template, learner’s instructions, gender-sensitiveness, context and content. In addition, education theory has been shifted from instructivism to constructivism which is being experimented and implemented by the Ministerial project entitled Teaching Quality Improvement (TQI partnering with the BOU School of Education with new texts. Time changes, new things are being adopted. Open School also requires revising its texts in relation to the government’s current agenda of implementing the digital Bangladesh. This study collects data from tutors, distance educators, writers and reviewers and finally develops a framework for revising the OS SLMs at a digital environment.

  3. A New Polyoxypregnane Glycoside from the Roots of Dregea volubilis (L.f Benth. ex Hook. f and its Chondroprotective Effect

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    Saksri Sanyacharernkul

    2009-01-01

    Full Text Available Problem statement: Dregea genus (Asclepiadaceae is well known for the rich of steroid pregnane contents and these plants are famous for the potential to be applied as alternative biological activities. Dregea volubilis is the only species of Dregea genus in Thailand. The chemical and biological investigations of this plant are interesting to bioassay-guided fractionation, particularly chondroprotective effect. Approach: The research was carried out to extract, isolate, purify and elucidate structure of the active compound from the roots Dregea volubilis. Both of the solvent extracts and isolated compound were evaluated with kinds of chondroprotection. i.e., S-GAG, HA, UA and production of matrix metalloproteinase-2 (MMP-2. Results: Polyoxypregnane glycoside (PGG or 12-0-benzoyl-8, 11-ditigloyl-3β, 8β, 11β, 12β, 14β-pentahydroxy-pregn-14-ol, 20-one,-3-0-methyl-β-D-allopyranosyl (1→4-β-D-thevetopyranoside was isolated from the active ethyl acetate extract of the roots Dregea volubilis. The spectroscopic techniques were provided for success in structure determination. In addition, a new compound was the most powerful to biological activities. Chondroprotective effect of PPG on the degradation of sulfated glycosaminoglycan (S-GAG, hyaluronan (HA, uronic acid (UA and production of matrix metalloproteinase-2 (MMP-2 in interleukin-1β (IL-1β-stimulated porcine articular cartilage were also assessed. PGG was interestingly effective in reducing IL-1β induced S-GAG, HA release from cartilage explant and MMP-2 activity. Furthermore, PPG can reverse effect of IL-1β-reduced the levels of uronic acid remaining in cartilage tissue. Conclusion: The PGG was possessed a potent chondroprotective activity using the IL-1β stimulated cartilage explant model. Therefore, it is possible to use this compound as a new pharmacological agent for the management of degenerative joint diseases.

  4. Effects of increasing inclusion rates of a low-fat distillers dried grains with solubles (LF-DDGS) in finishing broiler diets

    Science.gov (United States)

    The biodiesel industry is now removing corn oil from distillers dried grains with solubles (DDGS) in order to meet increasing demand. The objectives of this study were to determine the maximum inclusion rates in broiler diets fed from 28 to 42 d of age in the finishing phases of production and the ...

  5. Productos del mercado artesanal en la ciudad de Leticia (Amazonia colombiana) elaborados con especies de bosques de Mauritia flexuosa L.f.

    OpenAIRE

    Sandra Lorena Franco Arango; Mauricio Sánchez Sáenz; Ligia Estela Urrego Giraldo; Andrea Galeano González; María Cristina Peñuela-Mora

    2015-01-01

    Los productos forestales no maderables (PFNMs) han tomado fuerza como alternativa para la conservación de ecosistemas y la generación de ingresos económicos en las poblaciones asociadas. Se presenta un mayor interés sobre los productos que provienen de bosques oligárquicos (poco diversos y con alta densidad relativa), especialmente los dominados por palmas, como por ejemplo los bosques de Mauritia flexuosa (aguajales, cananguchales o buritizales). En el mercado artesanal de Leticia (Amazonia ...

  6. The clinical research of elemene emulsion combined with F0LF0X4 regimen in the treatment of advanced gastric carcinoma

    Institute of Scientific and Technical Information of China (English)

    Yanzhi Bi; Dongxiang Zeng; Yang Ling

    2012-01-01

    Objective: The aim of this study was to observe the effects and adverse reactions of elemene emulsion added to the chemotherapy in the treatment of advanced gastric carcinoma (AGC). Methods: Forty-nine patients were divided randomly into two groups, elemene emulsion group (25 cases, treated with chemotherapy and elemene emulsion) and chemotherapy group (24 cases, treated with chemotherapy only). All patients received chemotherapy. The clinical effects and adverse reactions were evaluated after four cycles. Results: The response rate (RR) were 60% in elemene emulsion group and 41.7% in chemotherapy group respectively (P < 0.05). The median time to progression and overall survival in elemene emulsion group and in chemotherapy group were 7.1 months and 11.0 months vs 5.2 months and 9.3 months (P < 0.05). A lower rate of neutropenia, nausea, vomiting and diarrhea occurred in elemene emulsion group compared with chemotherapy group (P < 0.05), and there was significant difference in the elevation of life quality as well (48% vs 25%; P < 0.05). Conclusion: Elemene emulsion in combination with FOLFOX4 regimen can improve the efficacy, decrease the incidence of side effects of chemotherapy and elevate the life quality and prolong the survival time in AGC.

  7. Engaging plant anatomy and local knowledge on the buriti palm (Mauritia flexuosa L.f.: Arecaceae): the microscopic world meets the golden grass artisan's perspective

    Science.gov (United States)

    Viana, Rebeca V. R.; Scatena, Vera L.; Eichemberg, Mayra T.; Sano, Paulo T.

    2016-11-01

    Considering that both Western Science and Local Knowledge Systems share a common ground—observations of the natural world—the dialogue between them should not only be possible, but fruitful. Local communities whose livelihoods depend on traditional uses of the local biodiversity not only develop knowledge about nature, making several uses of such knowledge, but, with that process, several inquiries about nature can be raised. Here we present our experience with the engagement of Western Science with golden grass artisan's knowledge about the buriti palm (M. flexuosa). We applied 25 semi-directive interviews, combined with field diary and participative observation, in two quilombola communities from Jalapão region (Central-Brazil). One of the inquiries that emerged from the artisan's perspectives was about the differences between male and female buriti palms' fiber. We then engaged both local and scientific perspectives regarding this issue using plant anatomy as a dialogue instrument. Here we describe this experience and resort to Paulo Freire's ideas on dialogue to argue that, to integrate Western Science and Local Knowledge Systems in a collaborative and contextualized perspective, the research should be faced as a mutual learning practice.

  8. An Experimental Investigation of the Improvement in the Reception of TM (Transverse Magnetic)-Polarized LF (Low-Frequency) Waves with a Two-Element Spaced Array.

    Science.gov (United States)

    1984-05-01

    7 AD-A142 478 AN EXPERIENTAL INVESTIATION OF THE PROVENT IN THE 1/lRECEPT IO OF71 II I lii EAPULSE INC KFORD MASS F U TINGLEY ET AL. MAT 64 RADC-11...the distance, and the intrusion of a hill well into the first Fresnel zone rendered the telemetry link marginal. Intersite communication using citizen

  9. Genetic resources of the functional food, teramnus labialis (L.f.) spreng for improving seed number, flavonol content, oil %, and fatty acid compositions

    Science.gov (United States)

    Teramnus labialis is used as food in India and has potential to be used as a functional food vegetable in the U.S.A. Photoperiod-sensitive T. labialis accessions were grown in the greenhouse from 2010 to 2011 and evaluated for flavonol content, oil %, and fatty acid compositions. Significant variati...

  10. Prediction of Starch, Soluble Sugars and Amino Acids in Potatoes (Solanum tuberosum L.) Using Hyperspectral Imaging, Dielectric and LF-NMR Methodologies

    DEFF Research Database (Denmark)

    Kjær, Anders; Nielsen, Glenn; Stærke, Søren

    2016-01-01

    was designed to evaluate five different scanning methods for their potential use in potato assessment and sorting. Two methods were based on hyperspectral imaging, two were based on dielectric/bio-impedance and one was based on low-field nuclear magnetic resonance. A set of 60 potatoes of 10 different......, conductivity of pressed cell sap and cell sizes. Results showed that most types of spectral analyses had relatively high potential for predicting the starch-related parameters and medium potential for predicting the concentration of the reducing sugars fructose and glucose. Most methods showed medium potential...... for prediction of several amino acids, including asparagine, which showed particularly promising predictions in the hyperspectral analyses of intact potatoes. The presented screening study enabled us to perform robust choices for the further development and optimization of the methods and instruments...

  11. Klofið land, klofið sjálf. Leonard Cohen og leitin að kanadískri bókmenntahefð

    OpenAIRE

    Valur Snær Gunnarsson 1976

    2012-01-01

    Ritgerðin fjallar um höfundarverk Leonard Cohen, jafnt skáldsögur, ljóðabækur og hljómplötur, í samhengi við þann klofning í kanadískum bókmenntum sem fræðimenn telja að hafi einkennt þær allt frá seinni heimsstyrjöld. Í fyrri hlutanum er sjónum beint að uppvexti Cohen í Montreal, þar sem enskum og frönskum áhrifum, gyðingdómi og kaþólsku ægir saman. Sérstaklega verður skoðað samband hans við tvo menn, föðurinn sem kynnti hann fyrir enskum bókmenntum og hefðum, og móðurafann sem lagði áhe...

  12. 78 FR 66945 - Proposed Collection; 60-Day Comment request: Gulf Long-Term Follow-Up Study (GuLF STUDY)

    Science.gov (United States)

    2013-11-07

    ... HUMAN SERVICES National Institutes of Health Proposed Collection; 60-Day Comment request: Gulf Long-Term...), will publish periodic summaries of proposed projects to be submitted to the Office of Management and... create a resource for additional collaborative research on focused hypotheses or subgroups. Exposures...

  13. Aquatic macrophytes in the large, sub-tropical Itaipu Reservoir, Brazil

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    Roger Paulo Mormul

    2010-12-01

    Full Text Available In the last three decades, rapid assessment surveys have become an important approach for measuring aquatic ecosystem biodiversity. These methods can be used to detect anthropogenic impacts and recognize local or global species extinctions. We present a floristic survey of the aquatic macrophytes along the Brazilian margin of the Itaipu Reservoir conducted in 2008 and compare this with a floristic survey conducted ten years earlier. We used ordination analysis to determine whether assemblage composition differed among reservoir arms. Macrophyte species were sampled in each of the 235 sampling stations using a boat, which was positioned inside three places of each macrophyte stand to record species and search for small plants. We also collected submerged plants using a rake with the boat moving at constant velocity for ten minutes. We assigned individual macrophyte species to life form and identified representative species for each life form. A total of 87 macrophyte taxa were identified. The "emergent" life forms contained the highest number of species, followed by "rooted submerged" life forms. The extensive survey of macrophytes undertaken in September 2008 recorded more species than a survey conducted between 1995 and 1998. This could be due to changes in water physico-chemistry, disturbances due to water drawdown and the long period between surveys, which may have allowed natural colonization by other species. Additionally, differences in the classification systems and taxonomic resolution used in the surveys may account for differences in the number of species recorded. Assemblage composition varied among the arms and was affected by underwater radiation (as measured using a Secchi disk and fetch. Five non-native species were found. Two of these non-native species (Urochloa subquadripara and Hydrilla verticillata are of special concern because they have a high frequency of occurrence and occupy large marginal areas of the reservoir. Future

  14. NCBI nr-aa BLAST: CBRC-DDIS-10-0000 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DDIS-10-0000 ref|YP_203357.1| cytochrome c oxidase subunit 3 [Mortierella vert...icillata] gb|AAW51694.1| cytochrome c oxidase subunit 3 [Mortierella verticillata] YP_203357.1 1e-25 43% ...

  15. 150t EAF-LF/VD-CC流程生产石油套管钢39Mn2V的工艺实践%Technological practice of oil casing steel 39Mn2V with 150 t EAF-LF/VD-CC route

    Institute of Scientific and Technical Information of China (English)

    唐海燕; 李京社; 孙开明; 温德松; 张露; 王振祥

    2007-01-01

    天津钢管集团有限公司炼钢厂生产39Mn2V石油套管钢是采用EAF全程泡沫渣埋弧操作,EBT出钢合金化、LF复合精炼渣精炼,VD处理时间不小于12 min,连铸全程保护浇铸.连铸坯的平均w(O)为9.O×10-6,低倍组织均匀细致,表面质量良好,轧管各项技术指标均达到API SPEC 5CT要求.

  16. Anatomical and ultrastructural aspects of leaf galls in Ficus microcarpa L.f. (Moraceae induced by Gynaikothrips ficorum Marchal (Thysanoptera Aspectos anatômicos e ultra-estruturais de galhas foliares em Ficus microcarpa L.f. (Moraceae causadas por Gynaikothrips ficorum Marchal (Thysanoptera

    Directory of Open Access Journals (Sweden)

    Silvana Cristina P. M. de Souza

    2000-04-01

    Full Text Available Several species of Ficus present leaf galls and the goal of this research is to study the structural alterations involved in the formation of leaf galls caused by Gynaikothrips ficorum on F. microcarpa, an ornamental plant. The galls of young and mature leaves were separated into two developmental stages based on the presence of lesions on leaf lamina and the degree of leaf folding. Swellings of the lamina were observed in young and mature leaves during gall development which coincided with the areas of cellular hypertrophy and tissue hyperplasia. Swellings were detected in a greater amount and more precociously on young leaves when compared to mature ones. In young leaves, the cecidogenetic responses were quicker and led to further structural differences because younger cells are not completely differentiated. Cell hypertrophy and tissue hyperplasia were striking processes involved in the ontogenesis of the studied gall, similar to other galls induced by thrips. Nevertheless, in spite of the numerous sites of feeding and the wide area of attack, F microcarpa galls can be considered rudimentary, since no new tissue differentiation was observed.Galhas foliares são comuns em diversas espécies de Ficus. O objetivo deste trabalho é estudar as alterações estruturais envolvidas na formação das galhas foliares induzidas por Gynaikothripsficorum (Thysanoptera em Ficus microcarpa, uma planta ornamental. As galhas de folhas jovens e maduras foram separadas em dois estádios de desenvolvimento, com base na presença de lesões e no grau de dobramento da lâmina. Durante o desenvolvimento da galha formam-se intumescimentos na lâmina tanto nas folhas jovens quanto nas maduras, os quais coincidem com as áreas de hipertrofia celular e hiperplasia dos tecidos. Estes intumescimentos foram detectados em maior quantidade e mais precocemente em folhas jovens, que, por possuirem células menos diferenciadas, mostraram respostas cecidogenéticas mais rápidas. A hipertrofia e, a hiperplasia foram os processos marcantes na ontogênese desta galha, assim como ocorre em outras induzidas por tripes. Entretanto, apesar dos numerosos sítios de alimentação e a grande área de ataque, as galhas foliares de F. microcarpa podem ser consideradas rudimentares, pois não se observa a diferenciação de novos tecidos.

  17. Turboatomizador e repasse com pistola manual na cobertura de pulverização de agrotóxicos em caquizeiro (Diospyros kaki L.f. Air-jet sprayer and spray gun in the pesticide spray coverage of persimmon (Diospyros kaki L.f. trees

    Directory of Open Access Journals (Sweden)

    Leandro Riyuiti Higashibara

    2013-05-01

    Full Text Available A cultura do caquizeiro vem crescendo em importância no Brasil e, com o aumento do cultivo, tem havido também incremento dos problemas relacionados a doenças e pragas. A pulverização de agrotóxicos é o método mais utilizado na prevenção e no controle desses problemas e a principal forma de aplicação é a pulverização hidráulica com auxílio de fluxo de ar. O objetivo deste trabalho foi avaliar o efeito da variação da taxa de aplicação e do repasse na parte interna das plantas em pulverização com pistola manual, na cobertura de calda das folhas. A pulverização foi realizada em plantas de caquizeiro com 17 anos, utilizando-se equipamento composto de trator e pulverizador turboatomizador. A aplicação foi realizada com duas velocidades de avanço do equipamento, o que promoveu diferentes taxas de aplicação. Para a realização do repasse, utilizou-se pistola manual acoplada ao mesmo equipamento. Para a avaliação da cobertura, utilizou-se calda preparada com corante fluorescente dissolvido em água. Foram coletadas folhas nas posições externa e interna das plantas. Posteriormente, em sala escura e com iluminação ultravioleta para destacar a área atingida pelo corante fluorescente, as superfícies abaxial e adaxial de cada folha foram fotografadas com câmera digital. Cada imagem gerada foi submetida à análise pelo software SIARCS 3.0, resultando em porcentagem de cobertura. O delineamento experimental utilizado foi de blocos ao acaso em parcelas subsubdivididas com quatro repetições, sendo os tratamentos arranjados em esquema fatorial (taxas alta e baixa de aplicação, com e sem repasse manual, localizações interna e externa da folha na planta. Foi avaliada a cobertura das superfícies abaxial e adaxial das folhas. Houve interação significativa entre tratamentos (taxa de aplicação e repasse manual, posição e superfície das folhas. A maior taxa de aplicação promoveu aumento significativo da cobertura na superfície abaxial das folhas, independentemente da posição destas na planta (interna ou externa. Houve efeito do repasse com pistola na cobertura da superfície abaxial das folhas situadas na posição interna, mas não na externa à planta. Considerando que a maioria dos agentes causadores de danos fitossanitários inicia a ocorrência na superfície abaxial das folhas, pode-se concluir que o repasse manual foi mais eficiente do que o aumento da taxa de aplicação, visando à cobertura nas folhas posicionadas no interior da planta.Persimmon crop is growing in importance in Brazil, and with the increase of the production area there also has been an increase in the problems related to diseases and pests. For the control and prevention of these problems, most often it is used pesticides spray, and one of the main techniques is the spray with the aid of air flow. The objective of this study was to evaluate the effects of changes in the application rate and transfer with a spray gun on the leaves mixture coverage. The spraying was performed using tractor and air flow sprayer. The application was performed with two forward speeds of the equipment, leading to different application rates. The transfer was made with a spray gun attached to the same equipment. For the assessment of the coverage it was used a mixture prepared with fluorescent tracer dissolved in water. Leaves were collected in the inside and outside of the tree. Later, in dark room with ultraviolet lighting to highlight the area affected by the fluorescent tracer, the upper and lower surfaces of each leaf were photographed with a digital camera. Each image was subjected to analysis generated by software SIARCS 3.0, resulting in percentage of coverage. It was used a randomized block design with subdivided parcels with four replications, being the treatments in a 2x2x2 factorial scheme (high or low application rate, with or without transfer, inside or outside the tree. The evaluations were made in the upper and lower leaf surfaces. There was significant interaction between treatments (application rate and spray gun transfer, leaf position and surface. High application rate promoted a significant increase in the coverage of the lower leaves, regardless the site in the plant (inside or outside. The inside leaves had a better coverage with the spray gun transfer. As most of the agents of plant damage begin to occur in the lower surface of leaves, it can conclude that the spray gun transfer was more effective to cover the leaves inside the plant than increasing the application rate.

  18. 废钢+铁水-50 t EAF-LF-VD流程GCr15轴承钢中的非金属夹杂物行为%Behavior of Non-Metallic Inclusions in GCr15 Bearing Steel Steelmaking by Scrap + Hot Metal-50 t EAF-LF-VD Flow Sheet

    Institute of Scientific and Technical Information of China (English)

    杨密平; 吴兵; 林腾昌; 安杰; 马传庆

    2014-01-01

    通过50 t EAF配加30 ~40 t铁水和12~16 t优质废钢,EBT无渣出钢,加150 ~ 200 kg钢芯铝预脱氧,LF用SiC扩散脱氧,控制精炼渣碱度4.0~5.9,VD前后软吹氩、连铸保护浇铸和电磁搅拌等工艺措施,GCr15轴承钢轧材中的氧含量为8×10-6 ~9×10-6.分析结果表明,LF前至VD后钢中夹杂物尺寸一般≤10 μm,最大尺寸40μm,大部分夹杂物尺寸为3~6 μm;LF前主要夹杂物为Al2O3,镁铝尖晶石,硫化物,Cr2O3,TiO2;VD前后为镁铝尖晶石,CaS和MgO.

  19. Biological Control of Aquatic Plants with Pathogenic Fungi

    Science.gov (United States)

    1981-01-01

    been found thus far in nature or under arti- ficial treatments of uredia- bearing leaves of waterhyacinth in labora- tory. In the absence of knowledge...of these bacteria on healthy hydrilla. D5 Table DI Description of Seven Isolated Bacteria Grown on NA Plates Isolate 1 White, gummy , some slime...6 White, very slow growth, myceloid 7 Pink, some slime, gummy , slow growth, myceloid Table D2 Visual Comparison of Hydrilla Sprigs Three Weeks After

  20. 采煤沉陷区苦草的生物学特性及其竞争机制%Biological traits and competition mechanism of Vallisneria natans in the mining subsidence area

    Institute of Scientific and Technical Information of China (English)

    张玉云; 万阳; 田焕新; 刘靓靓; 周忠泽

    2015-01-01

    采煤活动所致的动态沉陷湿地具有动态性、高潜水位和生态脆弱性等特点,探明此类人工重构湿地的水生植物群落的生物学特性及其竞争机制,有助于为采煤沉陷区湿地水生植物群落修复提供科学依据.本研究以淮南潘集区人工构建的苦草群落(Ass.Vallisneria natans)为倒,采用样方调查法,研究苦草的生物学特性及其与其他水生植物的竞争机制.结果表明,1)沉陷区苦草的生物学特性与浅水湖泊较一致.主要表现在苦草的繁殖方式、传粉方式、生活史方面,但研究区苦草以有性繁殖为主.2)沉陷区苦草能与以种子萌发形成的水烛群丛(Ass.Typha angustifolia)共生,但生物量低;在与形成两年以上的水烛群丛竞争中处于劣势,在水烛群丛盖度达到90%时苦草逐渐消失.3)沉陷区苦草在与浮叶植物莕菜群丛(Ass.Nymphoides peltatum)、苹群丛(Ass.Marsilea quadrifolia)竞争中处于弱势,其盖度、株高、生物量等指标都明显下降.4)沉陷区苦草在与沉水植物竹叶眼子菜群丛(Ass.Potamogeton malaianus)竞争中处于劣势,苦草与沉水植物黑藻群丛(Ass.Hydrilla verticillata)、狐尾藻群丛(Ass.Myrioph yllum verticillatum)、大茨藻群丛(Ass.Najas marina)、金鱼藻群丛(Ass.Ceratophyllum demersum)占据不同水层空间,种间竞争不强烈,能较好地共存.总之,苦草较之于相似生长型的植物具有较明显的竞争优势,对于具有不同生活型,尤其是挺水植物和浮叶植物,处于竞争劣势.

  1. 连续可调式沉水植物网床对河道水质的修复%Bioremediation of river water quality by consecutively adjustable submerged vegetation net

    Institute of Scientific and Technical Information of China (English)

    吴海龙; 霍元子; 邵留; 王阳阳; 于克锋; 何培民; 温文科

    2012-01-01

    A series of consecutively adjustable submerged vegetation nets were constructed in a polluted shallow river with a length of about 200 m and nearby the water resource protection area of Taihu Lake in East China, forming an aquatic vegetation consisted of submerged plant species Ca-bomba caroliniana, Vallisneria natans, Elodea nuttallii, Hydrilla verticillata, and Potamogeton cris-pus. The water quality indices including total nitrogen (TN), ammonium nitrogen (NH4+-N) , nitrite nitrogen (NO2--N) , nitrate nitrogen (NO3--N) , total phosphorus (TP) , and phosphate (PO43-P) were monitored, and the bioremediation effect of the vegetation nets was evaluated. After setting up the vegetation nets, the Secchi depth (SD) of the river changed from 0.5 m to 1.7-1.8 m, and the TN and TP concentrations 15 and 20 days after the nets constructed decreased by 35. 6% and 66.3% , and 29.4% and 63. 2% , respectively. After five months, the concentrations of NH4+ -N, NO2--N, NO3--N, TN, TP, and PO43-P decreased by 92. 4% , 76. 8% , 72. 7% , 73.9% , 90. 5% , and 92. 0% , respectively. This study showed that consecutively adjustable submerged vegetation net could be a potential approach for treating polluted river waters, particularly for the bioremediation of polluted small landscape shallow water bodies.%在太湖贡湖水源保护区陆域的一条长约200 m的污染河道内构建了一系列连续可调式沉水植物网床,形成了以菊花草、苦草、伊乐藻、轮叶黑藻和菹草等沉水植物构成的水生植物群落;跟踪监测了总氮(TN)、铵态氮(NH4 +-N)、亚硝态氮(NO2--N)、硝态氮(NO3--N)、总磷(TP)和磷酸盐(PO43--P)等水质指标,分析沉水植物网床引导沉水植被恢复对污染河道的水质修复效果.结果表明:沉水植被网床构建后,水体透明度显著升高,由修复前的0.5m提高到1.7 ~1.8 m;在沉水植被网床构建后的第5天和第20天,TN和TP的削减率分别为35.6%、66.3%和29.4%、63.2%;5

  2. Study on Application of Daphnia magna and Submerged Vegetation to Remediation of Landscape Water%食藻虫引导的沉水植被修复景观水体的应用研究

    Institute of Scientific and Technical Information of China (English)

    夏哲韬; 史惠祥; 李遥

    2011-01-01

    A field test of bioremediation of eutrophic water by Daphnia magna and submerged vegetation was conducted in Xixi wetland. Domesticated Daphnia magna was introduced into the water body of restoration area to ingest cyanobacteria and other phytoplanktons, and subsequently, submerged plants, such as Vallisneria, Hydrilla verticillata, Elodea canadensis and Potamogeton pectinatus were transplanted. From September 2010 to February 2011, water samples were monitored to investigate bioremediation efficiency and stability. The experiment data show that compared with the control area, Chl-a, TN, N03- -N, NH4+ -N, TP, PO43- -P and CODMn in the water body of restoration area are decreased by 45.6% , 68.8% , 97.6% , 71.5% , 65.7% , 68.5% and 67.4% respectively, DO is increased by 88.3% , and Secchi depth (SD) reaches over 2.0 m. Overall, the water quality meets the II to HI criteria specified in the national surface water quality standard. In March 2011, the inferior V water was introduced into the restoration area to test the effectiveness of the established experiment system. The results show that after one month, all the water-quality indices are significantly lower compared with the control area (P <0.01), TN, TP and CODMn are reduced by 78.2% , 50% and 43.7% , the DO is increased by 22.4% , and the SD reaches 1.5 m.%在西溪湿地进行了食藻虫引导沉水植被生态修复富营养化水体的现场试验.在修复区水体中引入经过驯化的食藻虫来滤食蓝藻等浮游藻类,然后移植苦草、轮叶黑藻、伊乐藻、龙须眼子菜等沉水植物.于2010年9月-2011年2月,对水体的水质指标进行了逐月监测,分析对西溪湿地水体的净化效果及其稳定性.结果表明:修复区水体的叶绿素a、TN、NO3- -N、NH4+ -N、TP、PO43- -P和CODMn浓度相比于对照区分别低45.6%、68.8%、97.6%、71.5%、65.7%、68.5%和67.4%,DO增加了88.3%,水体透明度达到2.0m以上,水质达到国家地

  3. Virginia Coast Reserve 2007 Remote Sensing Experiment

    Science.gov (United States)

    2012-03-09

    Chenopodium ambrosiode Helianthemum canadense Allium canadense Crataegus viridis Hibiscus moscheutos Ambrosia artemisiifoli Cynodon dactylon... laevigata Galium pilosum Mitchella Repens Cenchrus tribuloides Galium tinctorium Mollugo Verticillata 13 Genus Species Genus Species Genus

  4. Occurrence of green alga Ernodesmisverticillata (Kuetzing) Boergesen at Malvan (Maharashtra coast)

    Digital Repository Service at National Institute of Oceanography (India)

    Untawale, A.G.; Agadi, V.V.

    The occurrence of Ernodesmis verticillata belonging to Chlorophyta has been recorded for the first time from Malvan, along the Maharashtra Coast. The general distribution, habit and habitat of this species are given. During the recent survey...

  5. Fumigant toxicity of five essential oils rich in ketones against Sitophilus zeamais (Motschulsky)

    OpenAIRE

    Herrera, J. M.; M.P Zunino; Y Massuh; R.P Pizzollito; J.S Dambolena; N.A Gañan; Zygadlo, J. A.

    2014-01-01

    Essential oils (EOs) and individual compounds act as fumigants against insects found in stored products. In fumigant assays, Sitophilus zeamais Motschulsky adults were treated with essential oils derived from Aphyllocladus decussatus Hieron, Aloysia polystachya Griseb, Minthostachys verticillata Griseb Epling and Tagetes minuta L , which are rich in ketones and their major components: a- thujone, R-carvone, S-carvone, (-) menthone, R (+) pulegone and E-Z- ocimenone. M. verticillata oil was th...

  6. LF-NMR Studies of Variations of Different Water States during Controlled Freezing Point Storage of Beef%低场核磁共振分析冰温牛肉中不同状态水分变化

    Institute of Scientific and Technical Information of China (English)

    许倩; 朱秋劲; 叶春; 王文秀

    2013-01-01

    利用低场核磁共振技术研究冰温贮藏条件下无包装与真空包装牛肉在贮藏过程中水分弛豫参数的变化规律.通过驰豫测定,结果显示新鲜牛肉中有3种不同活动状态的水分,即结合水、不易流动水和自由水,其对应的横向弛豫时间分别是T21、T22、T23.对弛豫参数与常见肉品质指标进行皮尔逊相关系数分析,揭示了两者间的相关性.结果表明:无包装牛肉自由水弛豫参数与其品质指标均有较强的相关性,而真空包装牛肉的弛豫参数与其品质指标的相关性均不强.对冰温贮藏牛肉的品质影响最大的水分是自由水,自由水的弛豫参数能够很好的用于无包装牛肉常规品质指标的研究.%The aim of this study to apply low field NMR to investigate regular variations of different relaxation parameters of water in vacuum packaged beef and non-vacuum packaged beef during controlled freezing point storage.NMR relaxation data indicated that there were three different types of water in fresh beef,namely bound water,free water and immobilized water with relaxation times of T21,T22 and T23 (x-axis),respectively.Correlation anaysis of three relaxation parameters and meat quality indices was conducted by measuring pearson correlation coefficients.Our results showed that the correlations between the studied relaxation parameters and meat quality indices were strong in non-vacuum packaged beef but not strong in acuum packaged beef.Free water had the biggest influce on meat quality during controlled freezing point storage of beef.The relaxation parameter of free water may hold great promise for potential applications to study quality indices of nonvacuum packaged beef.

  7. Türk Fermente Sucuğundan İzole Edilen Laktobasillerin Hidrojen Peroksit ve Hidrojen Sülfür Oluşturma Özelliklerinin Saptanması

    OpenAIRE

    Özdemir, Haydar

    2002-01-01

    This study was made with aim to determine H2O2 and H2S forming properties of some lactobacilli (L. sake, L curvatus, L. plantarum)isolated from Turkish fermented sausages. H2O2 forming properties of lactobacilli were determined in MRS-Pyrolusit agar and MRS-ABTS- Peroxidase agar while, H2S forming properties were determined in Lead Acetate agar, Lysine Iron agar and Triple Sugar Iron agar. The 49 strains (77.7%) in MRS-Pyrolusit agar, 52 strains (82.5%) in MRS-ABTS-Peroxidase agar were reacte...

  8. Research on the Inverter Air-plasma Cutting Machine of LF Contact Pilot Arc%逆变式空气等离子切割机低频引弧技术的研究

    Institute of Scientific and Technical Information of China (English)

    孙强; 刘延明

    2011-01-01

    研制了一种全数字化控制的新型逆变式空气等离子切割机.该等离子机采用非高频的引弧技术,有效地解决了传统技术中存在的高电磁干扰、引弧电路复杂、引弧成功率低等问题.设计以高性能数字信号处理器DSP2812作为主控制芯片,通过理论分析、计算与仿真、控制算法的改进等措施,解决了低频引弧技术中的问题.实验结果表明,该机具有可靠性高、电磁干扰小、一次引弧成功率高等特点.%The digital control system for air-plasma cutting inverter power supply has developed.This cutting machine based on low frequency contact pilot arc and has successfully solved the plenty of short-comings in conventional contact pilot arc technique, such as high electronic magnetic interference (EMI), complex circuit and lower successful ratio of pilot arc and so on.The high performance DSP2812 is utilized as the mainly control chip.By theory analyse, calculate and simulation, the difficulty in low frequency contact pilot arc has been solved.The experimental results verify that the power supply system has higher stability, lower EMI and higher successful ratio of pilot arc.

  9. l(52?\\si1ll:lfI?l:anST1:Tl;; F((,)ul;nal.lS7?ER[AM:JNOCl"cf3G2'NES ...

    African Journals Online (AJOL)

    Conclusion: This study shows that indigenous chickens in Kenya are carriers of Listeria ... that have been inadequately cooked and heat treated or those thafare consumed .... farmed perhaps due to stress of transportation to the market and ...

  10. The usage of activated carbon from teak sawdust (tectona grandis l.f.) and zeolite for the adsorption of Cr(VI) and its analysis using solid-phase spectrophotometry (sps)

    Science.gov (United States)

    Saputro, S.; Masykuri, M.; Mahardiani, L.; Mulyani, B.; Qorina, I.; Yoshimura, K.; Takehara, K.; Matsuoka, S.

    2017-02-01

    This study aims to evaluate the usage of teak sawdust and zeolite as an adsorbent of Cr(VI) ion; optimal composition ratio of the composite adsorbent; and the sensitivity of solid-phase spectrophotometry (SPS) as a method to determine the levels of Cr(VI) ion as an adsorption results of adsorbents. The adsorbent used were teak sawdust activated carbon and zeolite as a single and composite adsorbents. The teak sawdust carbonization using muffle furnace and then activated with H3PO4 10% while the zeolite with H2SO4 10%. The contacting process of the adsorbents with Cr(VI) was done by varying the compositions. Analysis of Cr(VI) level was done using SPS method. Characterization of adsorbent before and after being activated is done using a FTIR. The results showed that teak sawdust and zeolite can be used as adsorbents to adsorb Cr(VI) in the simulated liquid waste with the adsorption capacity 1.19 µg/g the optimum composition ratio of teak sawdust activated carbon and zeolite was 75%:25% with the percentage of adsorption was 62.72%. Solid-phase spectrophotometry is a sensitive method to analyze the decreased levels of Cr(VI) as an adsorption results in µg/L level with the limit of detection (LOD) was 0.03 µg/L.

  11. Efecto del alcohol y el hidróxido de sodio sobre la humectabilidad de la superficie de la madera de Tectona grandis L.F. de plantaciones de rápido crecimiento

    OpenAIRE

    Garro-Chavarría, Michael; Estudiante Instituto Tecnológico de Costa Rica, Escuela de Ingeniería Forestal; Cartago, Costa Rica

    2012-01-01

    Se determinó el efecto de la aplicación de dos soluciones químicas polares sobre la humectabilidad de la madera de Tectona grandis (teca). Las soluciones aplicadas fueron: solución concentrada de hidróxido de sodio y alcohol 90%. Se midió el ángulo de contacto entre la gota de agua y la superficie de la madera, en dos tipos de corte: tangencial y radial. Los resultados mostraron que el tratamiento de la superficie con hidróxido de sodio y alcohol aumenta significativamente la humectabilidad e...

  12. Effects of LF bracket on human dental pulp during orthodontic tooth movement%LF托槽在活髓牙排齐过程中对牙髓组织的影响

    Institute of Scientific and Technical Information of China (English)

    苏士文

    2010-01-01

    目的 通过观察LF托槽在最小摩擦力结扎时诱导型热休克蛋白70(HSP70)在正畸牙牙髓组织中的动态表达特点,研究LF托槽对牙髓组织的影响.方法 采用免疫组织化学方法观察LF托槽最小摩擦力结扎时对活髓牙髓组织正畸前、正畸后4、15d内诱导型HSP70在牙髓组织中的动态表达.结果 HSPT0的表达在牙髓组织受力早期呈强阳性,随后表达逐渐减弱,至14d为弱阳性.结论 LF托槽在早期排齐过程中对牙髓组织是安全有效的.

  13. Comparação de métodos para quebra de dormência das sementes de carvoeiro – Tachigali vulgaris L.F. Gomes da Silva e H.C. Lima (Família: Fabaceae – Caesalpinioideae(Nota Científica. Comparison of methods for breaking seed dormancy of carvoeiro –Tachigali vulgaris L.F. Gomes da Silva and H.C. Lima (Family: Fabaceae – Caesalpinioideae(Scientific Note.

    Directory of Open Access Journals (Sweden)

    Natashi Aparecida Lima PILON

    2012-06-01

    Full Text Available O cultivo de plantas nativas seja para a exploração econômica, para restauração ecológica ou qualquer outra finalidade, depende, antes de tudo, da possibilidade de propagação das espécies. A produção de mudas é o meio usual de propagação de plantas, considerando-se fundamental a germinação das sementes. Tachigali vulgaris é uma espécie arbórea de crescimento rápido e grande porte, com potencial madeireiro, cujas sementes apresentam dormência tegumentar, que dificulta a produção de mudas. Buscando encontrar a técnica mais eficaz para quebra de dormência de suas sementes, foram comparados cinco tratamentos com cinco repetições: controle, escarificação mecânica, imersão em água em temperatura ambiente (24 horas, escarificação mecânica + imersão em água em temperatura ambiente e escarificação ácida por imersão em ácido sulfúrico concentrado durante duas horas. Quase todas as sementes germinaram dentro de sete dias após a instalação do teste. As sementes sem tratamento ou imersas em água em temperatura ambiente durante 24 horas apresentaram porcentagem de germinação muito baixa, inferior a 25%. A germinação obtida para os tratamentos escarificação mecânica com lixa no 80 (84%, escarificação mecânica + imersão em água (72% e escarificação ácida (71% não diferiram significativamente entre si. Para a produção de mudas da espécie, portanto, recomenda-se a escarificação mecânica das sementes com lixa, pelo bom resultado de germinação e por dispensar o tratamento químico.The cultivation of native plants, either for economic exploitation, for ecological restoration or any other purpose, depends, above all, on the possibility of propagation of the species. The production of nursery-raised seedlings is the usual technique for propagation of tree species and seed germination is a critical step for that. The carvoeiro, Tachigali vulgaris, is a woody species of rapid growth and large size, with potential for timber, whose seeds have tegumentary dormancy, impairing the production of seedlings. Searching for the most effective technique to break dormancy of seeds, we compared five treatments with five replications each: control,mechanical scarification (sandpaper 80, immersion in water at room temperature (24 hours, mechanical scarification with sandpaper + immersion in water at room temperature, and immersion during two hours in concentrated sulfuric acid. Most seeds germinated in the first seven days after treatment application, ceasing the emergence of seedlings from this time. The germination rates of untreated seeds or simple immersion in water at room temperature were very low, less than 25%. The germination rates after mechanical scarification with sandpaper (84%, mechanical scarification with sandpaper + immersion in water (72% and acid scarification (71% did not differ significantly. Therefore, for the production of seedlings of the species, mechanical scarification of the seeds with sandpaper is recommend, which provides the tegumentary dormancy breaking and does not require chemical treatment.

  14. Comparação de métodos para quebra de dormência das sementes de carvoeiro – Tachigali vulgaris L.F. Gomes da Silva e H.C. Lima (Família: Fabaceae – Caesalpinioideae(Nota Científica). Comparison of methods for breaking seed dormancy of carvoeiro –Tachigali vulgaris L.F. Gomes da Silva and H.C. Lima (Family: Fabaceae – Caesalpinioideae)(Scientific Note).

    OpenAIRE

    Pilon, Natashi Aparecida de Lima [UNESP; Melo,Antônio Carlos Galvão de; Durigan, Giselda

    2012-01-01

    O cultivo de plantas nativas seja para a exploração econômica, para restauração ecológica ou qualquer outra finalidade, depende, antes de tudo, da possibilidade de propagação das espécies. A produção de mudas é o meio usual de propagação de plantas, considerando-se fundamental a germinação das sementes. Tachigali vulgaris é uma espécie arbórea de crescimento rápido e grande porte, com potencial madeireiro, cujas sementes apresentam dormência tegumentar, que dificulta a produção de mudas. Busc...

  15. A Taxonomy for Radio Location Fingerprinting

    DEFF Research Database (Denmark)

    Kjærgaard, Mikkel Baun

    2007-01-01

    Location Fingerprinting (LF) is a promising location technique for many awareness applications in pervasive computing. However, as research on LF systems goes beyond basic methods there is an increasing need for better comparison of proposed LF systems. Developers of LF systems are also lacking g...

  16. Environmental Assessment for Water Well Development at Buckley Air Force Base

    Science.gov (United States)

    2010-06-01

    Storage Tank (AST) 250,000-gallon capacity Water Pipelines * Well #1 to AST 1,633 linear feet (LF) Well #2 to AST intake pipeline 838 LF Well #3 to...capacity Water Pipelines * Well #1 to AST 2,940 LF Well #2 to AST intake pipeline 1,911 LF Well #3 to AST intake pipeline 2,888 LF AST to...Aboveground Storage Tank (AST) 250,000-gallon capacity Water Pipelines * Well #1 to AST 2,921 LF Well #2 to AST intake pipeline 1,698 LF Well #3 to

  17. Bioassay of Plant Growth Regulator Activity on Aquatic Plants

    Science.gov (United States)

    1990-07-01

    found at 7.5 pg 2 (data not shown). Presumed toxic effects such as brittleness and an increased red pigmentation kpresumably anthocyanins ) £-I were...more than 50 percent over untreated controls (Table 1). As described above, these hydrilla segments were red in color , and it was apparent that they

  18. Introduced aquatic plants and algae

    Science.gov (United States)

    Non-native aquatic plants such as waterhyacinth and hydrilla severely impair the uses of aquatic resources including recreational faculties (lakes, reservoirs, rivers) as well as timely delivery of irrigation water for agriculture. Costs associated with impacts and management of all types of aquatic...

  19. Final Environmental Assessment for the Installation of a Range Safety Lighting System at Avon Park Air Force Range, Florida

    Science.gov (United States)

    2010-08-01

    Sterna antillarum N T Mammals Florida panther Puma concolor coryi E E Florida black bear Ursus americanus floridans N T Reptiles and Amphibians ...Casuarina glauca Australian pine Dioscorea bulbifera Air potato Eichhornia crassipes Water hyacinth Eugenia uniflora Surinam cherry Hydrilla...Cuban treefrog (Osteophilus septentrionalis), Cuban brown anole (Anole sagrei) and the Indo-Pacific gecko (Hemidactylus garnoti) are some amphibian and

  20. In trans complementation of lethal factor reveal roles in colonization and dissemination in a murine mouse model.

    Directory of Open Access Journals (Sweden)

    David E Lowe

    Full Text Available Lethal factor (LF is a component of the B. anthracis exotoxin and critical for pathogenesis. The roles of LF in early anthrax pathogenesis, such as colonization and dissemination from the initial site of infection, are poorly understood. In mice models of infection, LF-deficient strains either have altered dissemination patterns or do not colonize, precluding analysis of the role of LF in colonization and dissemination from the portal of entry. Previous reports indicate rabbit and guinea pig models infected with LF-deficient strains have decreased virulence, yet the inability to use bioluminescent imaging techniques to track B. anthracis growth and dissemination in these hosts makes analysis of early pathogenesis challenging. In this study, the roles of LF early in infection were analyzed using bioluminescent signature tagged libraries of B. anthracis with varying ratios of LF-producing and LF-deficient clones. Populations where all clones produced LF and populations where only 40% of clones produce LF were equally virulent. The 40% LF-producing clones trans complimented the LF mutants and permitted them to colonize and disseminate. Decreases of the LF producing strains to 10% or 0.3% of the population led to increased host survival and decreased trans complementation of the LF mutants. A library with 10% LF producing clones could replicate and disseminate, but fewer clones disseminated and the mutant clones were less competitive than wild type. The inoculum with 0.3% LF producing clones could not colonize the host. This strongly suggests that between 10% and 0.3% of the population must produce LF in order to colonize. In total, these findings suggest that a threshold of LF must be produced in order for colonization and dissemination to occur in vivo. These observations suggest that LF has a major role in the early stages of colonization and dissemination.

  1. Anti-proliferative role of recombinant lethal toxin of Bacillus anthracis on primary mammary ductal carcinoma cells revealing its therapeutic potential.

    Science.gov (United States)

    Khandia, Rekha; Pattnaik, Bramhadev; Rajukumar, Katherukamem; Pateriya, Atul; Bhatia, Sandeep; Murugkar, Harshad; Prakash, Anil; Pradhan, Hare Krishna; Dhama, Kuldeep; Munjal, Ashok; Joshi, Sunil K

    2017-05-30

    Bacillus anthracis secretes three secretary proteins; lethal factor (LF), protective antigen (PA) and edema factor (EF). The LF has ability to check proliferation of mammary tumors, chiefly depending on mitogen activated protein kinase (MAPK) signaling pathway. Evaluation of therapeutic potential of recombinant LF (rLF), recombinant PA (rPA) and lethal toxin (rLF + rPA = LeTx) on the primary mammary ductal carcinoma cells revealed significant (p role of receptor for LF revealed c-Met receptor showing strongest affinity for LF (H bond = 19; Free energy = -773.96), followed by nerve growth factor receptor (NGFR) and human epidermal growth factor receptor (HER)-1. The study summarizes the use of rLF or LeTx as therapeutic molecule against primary mammary ductal carcinoma cells and also the c-Met as potential alternative receptor for LF to mediate and modulate PA independent signal transduction.

  2. 斧头湖水生植物多样性及群落演替研究%DIVERSITY VARIATION AND COMMUNITY SUCCESSION OF AQUATIC MACROPHYTES IN LAKE FUTOU

    Institute of Scientific and Technical Information of China (English)

    李中强; 任慧; 郝孟曦; 张萌; 徐军

    2012-01-01

    Due to extensive human activities, habitat loss and segmentation induce destruction and disappearance of aquatic plants in the shallow lakes. These activities cause secondary extinctions of aquatic animals, leading to loss of biodiversity in lakes and animal reproduction. Lake Futou, locates in southeastern Hubei Province, China, has the same functions, such as irrigation and aquaculture, as other shallow lakes in the middle and lower reaches of the Yangtze River. However, in recent 50 years, human disturbances, including reclamation, damming and over-fishing, have caused accelerated loss of diversity of aquatic plants. This worsened the ecosystem faction and seriously affected the productivity of aquatic resources and the quality of species. Thus, the aims of the this study were to understand the succession of aquatic plants in Lake Futou in recent decades and to comprehend the reasons associated with exploitation and utilization of resources in this lake. In order to investigate the aquatic plant diversity in Lake Futou, the species and community diversity were studied using field investigation in 2009, and the topology were analysed by geographical information system technique with ARC/INFO. Based on the results obtained from field investigation and previous studies, species diversity variations and succession of aquatic macrophytes community in Lake Futou from 1988 to 2009 were investigated and the reasons between the changes and some controlling factors were analyzed. The results showed that there were some variations on the number of species and dominant species of aquatic plants. Some species that were sensitive to disturbances such as Nymphaea stellata disappeared and some dominant species such as Potamogeton maackianus, Vallisneria denseserrulata and Hydrilla verticillata became sub-dominant species or accompanying species from 1988 to 2009. In addition, the introduced species such as Elodea nuttalli occurred in Lake Futou and became dominant species

  3. Substitution of live feed by formulated diet: effect on the growth and survival of Betta splendens (Regan) fry

    OpenAIRE

    Mandal, S.C.; Kholi, M.P.S.; N.P. Sahu; Prakash, C; Chadha, N.K.; S. Munilkumar; Das, P.

    2009-01-01

    The present work evaluates the effectiveness of partial or total replacement of live feed (LF) (Tubifex) together with formulated diet (FD) for Betta splendens. Three hundred Betta splendens fry of uniform size (mean weight 0.19±0.01g) were equally distributed in five treatment groups with three replicates in glass aquaria of 351itre capacity. Fishes were given diets at different ratio of LF and FD viz. T1(C) 100% LF; T2 75% LF, 25% FD; T3 50% LF, 50% FD; T4 25% LF, 75% FD and T5 100% FD and ...

  4. Genetic variability of lactoferrin content estimated by mid-infrared spectrometry in bovine milk.

    Science.gov (United States)

    Soyeurt, H; Colinet, F G; Arnould, V M-R; Dardenne, P; Bertozzi, C; Renaville, R; Portetelle, D; Gengler, N

    2007-09-01

    The effects of lactoferrin (LF) on the immune system have already been shown by many studies. Unfortunately, the current methods used to measure LF levels in milk do not permit the study of the genetic variability of lactoferrin or the performance of routine genetic evaluations. The first aim of this research was to derive a calibration equation permitting the prediction of LF in milk by mid-infrared spectrometry (MIR). The calibration with partial least squares on 69 samples showed a ratio of standard error of cross-validation to standard deviation equal to 1.98. Based on this value, the calibration equation was used to establish an LF indicator trait (predicted LF; pLF) on a large number of milk samples (n = 7,690). A subsequent study of its variability was conducted, which confirmed that stage of lactation and lactation number influence the overall pLF level. Small differences in mean pLF among 7 dairy breeds were also observed. The pLF content of Jersey milk was significantly higher than that in Holstein milk. Therefore, the choice of breed could change the expected LF level. Heritability estimated for pLF was 19.7%. The genetic and phenotypic correlations between somatic cell score and pLF were 0.04 and 0.26, respectively. As somatic cell score increases in presence of mastitis, this observation seems to indicate that pLF, or a function of observed pLF, compared with expected LF might have potential as an indicator of mastitis. The negative genetic correlation (-0.36) between milk yield and pLF could indicate an undesirable effect of selection for high milk production on the overall LF level.

  5. Ability of laser fluorescence device associated with fluorescent dyes in detecting and quantifying early smooth surface caries lesions

    Science.gov (United States)

    Mendes, Fausto M.; de Oliveira, Elisabeth; de Faria, Dalva L. A.; Nicolau, José

    2006-03-01

    A laser fluorescence (LF) device is a portable tool, but it does not measure minor mineral changes. Our in vitro study aim is to propose the association of an LF with two fluorescent dyes and to evaluate the performance in detecting and quantifying early demineralization. Artificial caries lesions are created in 40 primary canine teeth using a demineralizing solution (pH=4.8) for 12, 24, 48, and 96 h. LF measurements are performed with DIAGNOdent after demineralization in these samples and in 20 sound primary teeth. Measurements with LF with 0.2-mM tetrakis(N-methylpyridyl)porphyrin (LF TMPyP) and with 4-mM protoporphyrin IX (LF PPIX) are made. The amount of calcium loss is determined by atomic emission spectrometry. A correlation between LF and LF with dyes and mineral loss and receiver operating characteristics analysis are performed, as well as comparisons of sensitivity, specificity, and accuracy values. Significant correlation is obtained with LF TMPyP and mineral loss of lesions demineralized for 24, 48, and 96 h. Better performance is achieved with LF TMPyP for all parameters than with LF alone. LF PPIX does not present good results. In conclusion, LF TMPyP provides good performance in detecting and quantifying very early enamel caries lesions.

  6. Spherical harmonic decomposition on a cubic grid[04.25.Dm Numerical relativity; 02.60.Lj Ordinary and partial differential equations; boundary value problems; 02.70.Bf Finite-difference methods; 95.85.Sz Gravitational radiation, magnetic fields, and other observations; 97.60.Jd Neutron stars; 97.60.Lf Black holes;

    Energy Technology Data Exchange (ETDEWEB)

    Misner, Charles W [Department of Physics, University of Maryland, College Park, MD 20742-4111 (United States)

    2004-02-07

    A method is described by which a function defined on a cubic grid (as from a finite difference solution of a partial differential equation) can be resolved into spherical harmonic components at some fixed radius. This has applications to the treatment of boundary conditions imposed at radii larger than the size of the grid, following Abrahams et al (1998 Phys. Rev. Lett. 80 1812-5). In the method described here, the interpolation of the grid data to the integration 2-sphere is combined in the same step as the integration to extract the spherical harmonic amplitudes, which become sums over grid points. Coordinates adapted to the integration sphere are not needed.

  7. 基于trn L-F序列数据利用贝叶斯法推测罗汉松科的系统发育%Bayesian Inference of the Phylogeny of Podocarpaceae Based on trn L-F Sequence Date

    Institute of Scientific and Technical Information of China (English)

    苏应娟; 王艇; 陈国培; 安宇; 左武麟; 孙宇飞; 邓锋; 孙旭

    2004-01-01

    以黑松和Agathis australis为外类群,基于罗汉松科42种植物的叶绿体trnL-F序列数据通过贝叶斯法对该科的系统发育进行了推测.结果显示:①Phyllocladus为单系分支,位于罗汉松科的基部,是罗汉松科所有其余成员的姊妹群;②Nageia嵌套在罗汉松科内,同罗汉松属、Afrocarpus及Retrophyllum的关系较为密切;③Dacrycarpus为单系群且处于姊妹分支Falcatifolium-陆均松属的基部;④Lagarostrobs franklinii和Manoao colensoi应置于同一属Lagarostrobs内;⑤支持成立Halocarpus和Lepidothamnus属;⑥赞同Microstrobos和Microcachrys两属亲缘密切的观点.

  8. Comparative Study on the Treatment Efficiency Between LF Bracket and Conventional Pre-adjusted Bracket to the Treating Angle Class Ⅰ Non-extraction Cases%LF托槽与传统直丝弓托槽对非拔牙安氏1类病例的临床对比

    Institute of Scientific and Technical Information of China (English)

    孙洁; 张在德; 廖颖晖

    2011-01-01

    目的 比较LF托槽与传统直丝弓托槽对非拔牙安氏1类病例矫治的治疗时间、复诊次数、托槽脱落率及对牙周健康的影响.方法 随机选择40例12~16岁安氏1类非拔牙病例,分为LF托槽组(s组)与传统直丝弓托槽组(d组),分别记录矫治的总疗程时间、总复诊次数、托槽的总脱落率、及治疗1、3、6个月下切牙的牙龈指数(GI).结果 LF托槽组在矫治的总疗程、复诊次数、托槽总脱落率及牙龈指数明显低于传统直丝弓托槽组,经统计学分析其差异具有统计学意义(P<0.05).结论 LF托槽比传统直丝弓托槽对安氏1类非拔牙病例能更快地完成矫治,并更有利于牙周组织健康.

  9. Dicty_cDB: Contig-U12059-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available tkdkyylfffffilkwii iiinivldn*d*fqilikfkkylklf*kimlvikvpriiii*ef*ilvvhmvkiq*lf*n h*lnklein...kimlvikvpriiii*ef*ilvvhmvkiq*lf*n h*lnkleingsqmkk*lkyfiqiyl*mifqnylmkfii*ihiliksiiflhmvlvmdmki n*yqiiqlilyfh

  10. Dicty_cDB: SLC254 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available wgtt*lf**tnfi*ftsdnwatwn**sitlifiiiisiriiiiiiir i*w*ttrwnlntavtrtswtfirnhcaiq*TXINIMTSFTMTWMGF--- ---LNGAFPQ...tt*lf**tnfi*ftsdnwatwn**sitlifiiiisiriiiiiiir i*w*ttrwnlntavtrtswtfirnhcaiq*TXINIMTSFTMTWMGF--- ---t*wsistti

  11. Dicty_cDB: VSC148 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available Amino Acid sequence ---kkkkkkkkkkkkkkkkksiisq*lf*WFIYFNLKKKTNYTLKNHPNSIQKYSVIYTY I***kynnikiikkikknqllfklk ...nqlya*ksp*lnpkilcniyi yiiiki**yknnkknkkkpiii*ik Frame C: ---kkkkkkkkkkkkkkkkksiisq*lf*WFIYFNLKKKTNYTLKNHPNSIQKYSVIYTY I***kynniki

  12. Dicty_cDB: SLG109 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available HKIEEELRKMSKENPSFVTSFCLRSILYAKF*nflct ktycqrshsrshicighaies*nvaigygry**yrfnrccndersn*ilw*nhsilr*lf tw*kdr*ny...HFDLKHKIEEELRKMSKENPSFVTSFCLRSILYAKF*nflct ktycqrshsrshicighaies*nvaigygry**yrfnrccndersn*ilw*nhsilr*lf tw*k

  13. Dicty_cDB: SFD878 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ftinefnsmwwwlwk writrwswwsrwwsscwywcncwfcftrsrfhfnwrfkqlwl*lklknykyilnklkink v*lf...vnvkkk--- ---ytkqnknktkqnk*ndnlrfnlfnw*c*infkik*fiiiikfiifftinefnsmwww lwkwritrwswwsrwwsscwywcncwfcftrsrfhfn

  14. Bioactive recombinant human lactoferrin, derived from rice, stimulates mammalian cell growth.

    Science.gov (United States)

    Huang, N; Bethell, D; Card, C; Cornish, J; Marchbank, T; Wyatt, D; Mabery, K; Playford, R

    2008-01-01

    Today there is a concern about the use of animal source proteins and peptides in cell culture applications due to potential contamination by adventitious infectious pathogens. Recombinant production of these proteins using a plant host provides a safe and cost effective alternative. In this paper, we tested the effect of rice-derived recombinant human lactoferrin (rhLF) on mammalian cell growth. The purified rhLF was partially (about 50%) iron-saturated (pis-rhLF). Chemical modification of pis-rhLF generated apo-rhLF (90% iron saturation). All three forms of rhLF (pis, apo, holo) promoted growth of intestinal cells (HT-29) measured as [(3)H]-thymidine incorporation or viable cell count, but holo-rhLF was most effective. Holo-rhLF was further tested on hybridoma, osteoblast, and human embryonic kidney cells. Results showed that holo-rhLF promoted cell growth and reduced cell doubling time. The concentration of holo-rhLF in media was critical in promoting cell growth and each cell line had different concentration dependence with the most effective range from 5 to 200 mg/L. The effect of rhLF on antibody production was determined using a hybridoma cell line. Significantly, more antibodies were produced by cells grown with holo-rhLF than cells grown without holo-rhLF. We also compared the effect of holo-rhLF to that of human transferrin, a component commonly used in cell culture media as an iron source. Holo-rhLF was as effective as human transferrin in promoting cell growth and antibody production. Considering all the data obtained, we conclude that rhLF from rice is effective in promoting mammalian cell growth and increasing cell productivity.

  15. Dicty_cDB: SSI159 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available |AW682908.2 NF001G03LF1F1023 Developing leaf Medicago truncatula cDNA clone NF001... 6e-16 1 AW683430 |AW683430.2 NF011H04LF1F1043 Developing leaf Medicago truncatula cDNA clone NF011H04LF 5',

  16. Dicty_cDB: CHF675 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available complete sequence. 48 0.15 1 BG453581 |BG453581.1 NF092A07LF1F1050 Developing leaf Medicago truncatula cDNA...15 1 BG453480 |BG453480.1 NF092C08LF1F1055 Developing leaf Medicago truncatula cDNA clone NF092C08LF 5', mRN

  17. Dicty_cDB: CHA105 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 73-367476 strain AX4, complete sequence. 38 0.39 6 BE317387 |BE317387.2 NF068C10LF1F1070 Developing...49770.2 NF022C10LF1F1081 Developing leaf Medicago truncatula cDNA clone NF022C10LF 5', mRNA sequence. 36 0.5

  18. Dicty_cDB: SHF346 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 453581 |BG453581.1 NF092A07LF1F1050 Developing leaf Medicago truncatula cDNA clone NF092A07LF 5', mRNA seque...nce. 48 0.26 1 BG453480 |BG453480.1 NF092C08LF1F1055 Developing leaf Medicago tru

  19. Dicty_cDB: SHK887 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available BG453581 |BG453581.1 NF092A07LF1F1050 Developing leaf Medicago truncatula cDNA cl...one NF092A07LF 5', mRNA sequence. 48 0.26 1 BG453480 |BG453480.1 NF092C08LF1F1055 Developing leaf Medicago t

  20. Dicty_cDB: SFF194 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 124-6045772 strain AX4, complete sequence. 34 0.50 7 BE317387 |BE317387.2 NF068C10LF1F1070 Developing leaf M...249770 |BE249770.2 NF022C10LF1F1081 Developing leaf Medicago truncatula cDNA clone NF022C10LF 5', mRNA seque

  1. Large Fragment of DNA Polymerase I from Geobacillus sp. 777: Cloning and Comparison with DNA Polymerases I in Practical Applications.

    Science.gov (United States)

    Oscorbin, Igor P; Boyarskikh, Ulyana A; Filipenko, Maksim L

    2015-10-01

    A truncated gene of DNA polymerase I from the thermophilic bacteria Geobacillus sp. 777 encoding a large fragment of enzyme (LF Gss pol) was cloned and sequenced. The resulting sequence is 1776-bp long and encodes a 592 aa protein with a predicted molecular mass of 69.8 kDa. Enzyme was overexpressed in E. coli, purified by metal-chelate chromatography, and biochemically characterized. The specific activity of LF Gss pol is 104,000 U/mg (one unit of enzyme was defined as the amount of enzyme that incorporated 10 nmol of dNTP into acid insoluble material in 30 min at 65 °C). The properties of LF Gss pol were compared to commercially available large fragments of DNA polymerase I from G. stearothermophilus (LF Bst pol) and Bacillus smithii (LF Bsm pol). Studied enzymes showed maximum activity at similar pH and concentrations of monovalent/divalent ions, whereas LF Gss pol and LF Bst pol were more thermostable than LF Bsm pol. LF Gss pol is more resistant to enzyme inhibitors (SYBR Green I, heparin, ethanol, urea, blood plasma) in comparison with LF Bst pol and LF Bsm pol. LF Gss pol is also suitable for loop-mediated isothermal amplification and whole genome amplification of human genomic DNA.

  2. Quantification of specific E. coli in gut mucosa from Crohn's disease patients

    DEFF Research Database (Denmark)

    Jensen, Stina Rikke; Fink, Lisbeth Nielsen; Struve, Carsten

    2011-01-01

    We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli. The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal...

  3. Laser fluorescence detection of demineralization in artificial occlusal fissures.

    Science.gov (United States)

    Ferreira Zandoná, A G; Analoui, M; Schemehorn, B R; Eckert, G J; Stookey, G K

    1998-01-01

    Laser fluorescence (LF) has been used previously to detect early smooth-surface lesions. Although its use for detection of occlusal demineralization has been implicated, it has not been demonstrated. The aim of this study was to determine whether LF could detect demineralization in the base of artificial fissures. To employ LF for detection of occlusal demineralization an apparatus was devised to direct laser light into fissures and simultaneously detect fluorescence from the base of the fissures. Three groups (n = 40/group) of differing fissure types were prepared (straight wall, converging and diverging wall) with either a sound or lesioned base. One half of each group was examined with LF and dye-enhanced LF (DELF); the other half was examined with LF, exposed to plaque, examined with LF and DELF, air-polished and examined with DELF. All images were scored twice as either (1) carious; (2) sound, or (3) undetermined, by a group of 3 examiners. For fissures without plaque, the average sensitivity was higher for DELF (0.76) than for LF (0.54) (p DELF (0.64) than for LF (0.29) (p DELF (0.91) compared to LF (0.43); however, specificity was lower for DELF (0.05) compared to LF (0.55). When the fissures were air-polished and then examined with DELF, sensitivity averaged 0.82 (p DELF was a better diagnostic tool than LF for detection of demineralization in artificial fissures.

  4. Quantification of specific E. coli in gut mucosa from Crohn's disease patients

    DEFF Research Database (Denmark)

    Jensen, Stina Rikke; Fink, Lisbeth Nielsen; Struve, Carsten;

    2011-01-01

    We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli. The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal...

  5. Synbiotic administration of canine-derived strain Lactobacillus fermentum CCM 7421 and inulin to healthy dogs.

    Science.gov (United States)

    Strompfová, Viola; Lauková, Andrea; Cilik, Dušan

    2013-05-01

    The canine-derived strain Lactobacillus fermentum CCM 7421 has been demonstrated to exert certain health benefits as a probiotic in dogs. Synbiotic combinations are widely used but are rarely studied in dogs. In this study the prebiotic inulin in combination with L. fermentum CCM 7421 was tested for its effects on faecal microbial populations, faecal characteristics, and blood biochemistry in canine experiments. Healthy adult dogs (n = 36) were randomly assigned to 1 of 3 treatment groups (12 dogs/treatment): (i) the control group (C), (ii) the probiotic group (LF group: L. fermentum CCM 7421, 10(8) CFU/mL of Ringer buffer, 0.1 mL/kg of body mass), and (iii) the synbiotic group (LF+I group: L. fermentum CCM 7421 + inulin (I; Raftifeed IPS) added as 1% of diet). The experiment lasted for 7 weeks with a 2-week treatment period. We detected a significant increase of lactic acid bacteria (LF versus C, day 7; LF versus C and LF versus LF+I, days 28 and 49), a decrease of clostridia (LF versus C, day 14), a lower pH value (LF versus LF+I, day 28), and a higher ammonia concentration (LF versus LF+I, days 14 and 49) in faecal samples. The synbiotic LF+I combination did not intensify the probiotic L. fermentum CCM 7421 efficacy, but its slight laxative effect can be useful to prevent constipation, e.g., in senior dogs.

  6. Lactoferrin Directly Scavenges Hydroxyl Radicals and Undergoes Oxidative Self-Degradation: A Possible Role in Protection against Oxidative DNA Damage

    Directory of Open Access Journals (Sweden)

    Yuki Ogasawara

    2014-01-01

    Full Text Available In this study, we examined the protective effect of lactoferrin against DNA damage induced by various hydroxyl radical generation systems. Lactoferrin (LF was examined with regard to its potential role as a scavenger against radical oxygen species using bovine milk LF. Native LF, iron-saturated LF (holo-LF, and apolactoferrin (apo-LF effectively suppressed strand breaks in plasmid DNA due to hydroxyl radicals produced by the Fenton reaction. In addition, both native LF and holo-LF clearly protected calf thymus DNA from fragmentation due to ultraviolet irradiation in the presence of H2O2. We also demonstrated a protective effect of all three LF molecules against 8-hydroxydeoxyguanosine (8-OHdG formation in calf thymus DNA following ultraviolet (UV irradiation with H2O2. Our results clearly indicate that native LF has reactive oxygen species-scavenging ability, independent of its nature as a masking component for transient metals. We also demonstrated that the protective effect of LF against oxidative DNA damage is due to degradation of LF itself, which is more susceptible to degradation than other bovine milk proteins.

  7. Monitoring Invasive Aquatic Vegetation in Lake Okeechobee, Florida, Using NDVI Derived from Modis Data

    Science.gov (United States)

    Woods, Kate; Brozen, Madeline; Malik, Sadaf; Maki, Angela

    2009-01-01

    Lake Okeechobee, located in southern Florida, encompasses approximately 1,700 sq km and is a vital part of the Lake Okeechobee and Everglades ecosystem. Major cyanobacterial blooms have been documented in Lake Okeechobee since the 1970s and have continued to plague the ecosystem. Similarly, hydrilla, water hyacinth, and water lettuce have been documented in the lake and continue to threaten the ecosystem by their rapid growth. This study examines invasive aquatic vegetation occurrence through the use of the Normalized Difference Vegetation Index (NDVI) calculated on MOD09 surface reflectance imagery. Occurrence during 2008 was analyzed using the Time Series Product Tool (TSPT), a MATLAB-based program developed at John C. Stennis Space Center. This project tracked spatial and temporal variability of cyanobacterial blooms, and overgrowth of water lettuce, water hyacinth, and hydrilla. In addition, this study presents an application of Moderate Resolution Imaging Spectroradiometer (MODIS) data to assist in water quality management.

  8. Comparative analysis of virulence traits between a Legionella feeleii strain implicated in Pontiac fever and a strain that caused Legionnaires' disease.

    Science.gov (United States)

    Wang, Changle; Saito, Mitsumasa; Tanaka, Tamami; Amako, Kazunobu; Yoshida, Shin-ichi

    2015-12-01

    Legionella strains of the same species and serogroup are known to cause Legionnaires' disease (a potentially fatal atypical pneumonia) or Pontiac fever (a mild, flu-like disease), but the bacterial factors that define these dramatic differences in pathology have not been elucidated. To gain a better understanding of these factors, we compared the characteristics of Legionella feeleii strains that were isolated from either a sample of freshwater implicated in an outbreak of Pontiac fever (ATCC 35072, serogroup 1, LfPF), or a patient with Legionnaires' disease (ATCC 38549, serogroup 2, LfLD). Growth of LfPF and LfLD in BYE broth was slower than the positive control, Legionella pneumophila strain JR32. However, LfLD grew faster than LfPF at 42 °C. After in vitro infection to J774 murine or U937 human macrophage cell lines and A549 human lung epithelial cell line, LfLD showed a higher cell infection rate, stronger internalization by host cells, and greater cytotoxicity than that of LfPF. Large amounts of IL-6 and IL-8 were secreted by human host cells after infection with LfLD, but not with LfPF. LfLD possessed mono-polar flagellum while LfPF was unflagellated. When LfLD was cultured at 25, 30 and 37 °C, the bacteria had higher motility rate at lower temperatures. Based on our results, this is the first study that showed distinct characteristics between LfPF and LfLD, which may give important leads in elucidating differences in their virulence.

  9. Environmental Factors Influencing Blooms of a Neurotoxic Stigonematalan Cyanobacterium Responsible for Avian Vacuolar Myelinopathy

    Science.gov (United States)

    2013-01-01

    killing waterbirds and raptors , to an epiphytic cyanobacterium which grows primarily on nonindigenous submerged aquatic vegetation (SAV). Water- birds...intramyelinic edema, in the organisms’ CNS tissue, most commonly the brain (Thomas et al. 1998). AVM-afflicted birds display erratic behavior ; clinical symptoms... feeding trials. Gavage trials with a methanol extract of the hydrilla-UCB demonstrated that extract would cause clinical symptoms and AVM lesions in

  10. Point Intercept and Surface Observation GPS (SOG): A Comparison of Survey Methods - Lake Gaston, NC/VA

    Science.gov (United States)

    2008-11-01

    coverage by hydrilla was greater than 50% (littoral zone approximately 5,000 acres, Madsen 2007). Scientific name Common name Exotic/native Whole lake...vegetation in the lake. Scientific name Common name Monospecific acres Total acres Native emergent Echinodorus cordifolius creeping burhead...0.001 0.001 Eleocharis macrostachya flatstem spikerush 0.002 0.002 E. quadrangulata squarestem spikerush 2.66 7.21 Hibiscus spp. mallow 0.06 0.27

  11. Apparent Herbivory and Indigenous Pathogens of Invasive Flowering Rush (Butomus umbellatus L.) in the Pacific Northwest

    Science.gov (United States)

    2015-06-01

    hand or with a garden trowel. Though flowering rush spreads vegetatively and produces extensive underground rhizome systems, for the purposes of this...the Great Smoky Mountains National Park in 2004 (Baird et al. 2007). Both F. oxysporum and Phoma have broad host ranges, which may limit their...is dubious, considering that the majority of them have been tested on other plant species (hydrilla and Eurasian watermilfoil) with little success

  12. Incidence and Management Costs of Freshwater Aquatic Nuisance Species at Projects Operated by the U.S. Army Corps of Engineers

    Science.gov (United States)

    2010-07-01

    the occurrence of ANS impacts (Yes or No) from freshwater algae, large aquatic plants, fish , zebra mussels, Asiatic clams, water fleas, crayfish...investment in research and development, especially for nuisance aquatic plants, fresh- water clams, and certain invasive fish species. Even so, aggregate...water hyacinths, hydrilla, common reed, sea lamprey, and the zebra mussel are especially notorious for their costly impacts on freshwater services

  13. 伊乐藻的利用价值

    Institute of Scientific and Technical Information of China (English)

    杨清心

    1988-01-01

    伊乐藻(Elodea nuttallii)原产美洲,是一种速生高产的沉水高等植物,与我国淡水水域中分布的黑藻(Hydrilla verticilata)苦草(Vallisneria spiralis)同属水鳖科(Hydrocharitaceae),是草食性鱼类的优良饲料,又有净化水质的作用。

  14. La familia Valoniaceae (Chlorophyta en el estado de Bahía, Brasil: aspectos morfológicos y de distribución The family Valoniaceae (Chlorophyta in the state of Bahia, Brazil: morphological aspects and geographical distribution

    Directory of Open Access Journals (Sweden)

    Aigara Miranda Alves

    2010-08-01

    Full Text Available El trabajo contempla el estudio morfológico y de distribución de las especies de Valoniaceae presentes en la costa del Estado de Bahía, Brasil. Se analizaron en el estudio especímenes de herbario y muestras recolectadas entre 1994 y 2010 en un total de 29 localidades. Se recolectaron cuatro especies: Valonia aegagropila, V. macrophysa, V. ventricosay Ernodesmis verticillata, siendo esta última una nueva adición a la flora de macroalgas del Estado. Para la costa brasileña se documenta por la primera vez talos fértiles de E. verticillata y la presencia de cristales de sílice y carbonato de calcio en las células de los taxones estudiados. Se presentan descripciones, ilustraciones y datos de distribución geográfica para cada especie a lo largo de la costa brasileña, asi como comentarios de otras especies relacionadas. Valonia aegagropila presentó una amplia distribución a lo largo de la costa, V. macrophysa y V. ventricosa, se registraron desde la Bahía de Todos los Santos hasta las Islas Abrolhos, mientras que E. verticillata ocurrió aisladamente en las Islas de Itaparica y Madre de Deus, ambas en la Bahía de Todos los Santos.The present paper included morphological and geographical distribution aspects of the Valoniaceae species occurring in the littoral of Bahía , Brazil. Specimens from vouchers of herbaria and material collected at 29 sites between 1994 and 2010 were analyzed. Four species were identified: Valonia aegagropila, V. macrophysa, V. ventricosa and Ernodesmis verticillata, the latter is a new record for the marine algae of Bahía state. Fertile thalli of E. verticillata and the presence of silica and calcium carbonate crystals into their cells were documented for the first time for the Brazilian coast. Detailed descriptions, illustrations and geographical distribution data for each species through the brazilian coast, as well as, discussion with related taxa are presented. Valonia aegagropila showed a wide

  15. Identification of pneumococcal surface protein A as a lactoferrin-binding protein of Streptococcus pneumoniae.

    Science.gov (United States)

    Hammerschmidt, S; Bethe, G; Remane, P H; Chhatwal, G S

    1999-04-01

    Lactoferrin (Lf), an iron-sequestering glycoprotein, predominates in mucosal secretions, where the level of free extracellular iron (10(-18) M) is not sufficient for bacterial growth. This represents a mechanism of resistance to bacterial infections by prevention of colonization of the host by pathogens. In this study we were able to show that Streptococcus pneumoniae specifically recognizes and binds the iron carrier protein human Lf (hLf). Pretreatment of pneumococci with proteases reduced hLf binding significantly, indicating that the hLf receptor is proteinaceous. Binding assays performed with 63 clinical isolates belonging to different serotypes showed that 88% of the tested isolates interacted with hLf. Scatchard analysis showed the existence of two hLf-binding proteins with dissociation constants of 5.7 x 10(-8) and 2.74 x 10(-7) M. The receptors were purified by affinity chromatography, and internal sequence analysis revealed that one of the S. pneumoniae proteins was homologous to pneumococcal surface protein A (PspA). The function of PspA as an hLf-binding protein was confirmed by the ability of purified PspA to bind hLf and to competitively inhibit hLf binding to pneumococci. S. pneumoniae may use the hLf-PspA interaction to overcome the iron limitation at mucosal surfaces, and this might represent a potential virulence mechanism.

  16. Radioprotection of mice by lactoferrin against irradiation with sublethal X-rays.

    Science.gov (United States)

    Nishimura, Yoshikazu; Homma-Takeda, Shino; Kim, Hee-Sun; Kakuta, Izuru

    2014-03-01

    The influence of a host defense protein, lactoferrin (LF), contained in exocrine secretions such as milk, on radiation disorder was investigated. A total of 25 C3H/He mice in each of two groups were maintained with 0.1% LF-added and LF-free diets, respectively, for one month. The mice were then treated with single whole-body X-ray irradiation at a sublethal dose (6.8 Gy), and the survival rate after irradiation was investigated. The survival rate at 30 d after irradiation was relatively higher in the LF group than in the control group (LF-free), (85 and 62%, respectively). The body weight 15 d after X-ray irradiation was also significantly greater in the LF group than in the control group. The hemoglobin level and hematocrit value were higher in the LF group at 5 d before X-ray irradiation. Another 52 mice underwent whole-body X-ray irradiation at the sublethal dose (6.8 Gy), and then LF was intraperitoneally injected once at 4 mg/animal to half of them. The survival rate in LF-treated mice 30 d after irradiation was 92%, significantly higher than in mice treated with saline (50%) (P = 0.0012). In addition, LF showed hydroxyl radical scavenger activity in vitro. These findings suggest that LF may inhibit radiation damage.

  17. MiR-21 promotes fibrosis and hypertrophy of ligamentum flavum in lumbar spinal canal stenosis by activating IL-6 expression.

    Science.gov (United States)

    Sun, Chao; Tian, Jiwei; Liu, Xinhui; Guan, Guoping

    2017-08-26

    The molecular mechanism underlying the fibrosis of ligamentum flavum(LF) in patients with lumbar spinal canal stenosis(LSCS) remains unknown. MicroRNAs are reported to play important roles in regulating fibrosis in different organs. The present study aimed to identify fibrosis related miR-21 expression profile and investigate the pathological process of miR-21 in the fibrosis of LF hypertrophy and associated regulatory mechanisms. 15 patients with LSCS underwent surgical treatment were enrolled in this study. For the control group, 11 patients with lumbar disc herniation(LDH) was included. The LF thickness was measured on MRI. LF samples were obtained during the surgery. Fibrosis score was assessed by Masson's trichrome staining. The expression of miR-21 in LF tissues were determined by RT-PCR. Correlation among LF thickness, fibrosis score, and miR-21 expression was analyzed. In addition, Lentiviral vectors for miR-21 mimic were constructed and transfected into LF cells to examine the role of miR-21 in LF fibrosis. Types I and III collagen were used as indicators of fibrosis. IL-6 expression in LF cells after transfection was investigated by RT-PCR and ELISA. Patients in two groups showed similar outcomes regarding age, gender, level of LF tissue. The thickness and fibrosis score of LF in the LSCS group were significantly greater than those in LDH group (all P hypertrophy. Copyright © 2017 Elsevier Inc. All rights reserved.

  18. Lactoferrin interacts with bile acids and increases fecal cholesterol excretion in rats.

    Science.gov (United States)

    Nakamura, Kanae; Morishita, Satoru; Ono, Tomoji; Murakoshi, Michiaki; Sugiyama, Keikichi; Kato, Hisanori; Ikeda, Ikuo; Nishino, Hoyoku

    2017-02-01

    Lactoferrin (LF) is a multifunctional cationic protein (pI 8.2-8.9) in mammalian milk. We previously reported that enteric-LF prevented hypercholesterolemia and atherosclerosis in a diet-induced atherosclerosis model using Microminipig, although the underlying mechanisms remain unclear. Because LF is assumed to electrostatically interact with bile acids to inhibit intestinal cholesterol absorption, LF could promote cholesterol excretion. In this study, we assessed the interaction between LF and taurocholate in vitro, and the effect of LF on cholesterol excretion in rats. The binding rate of taurocholate to LF was significantly higher than that to transferrin (pI 5.2-6.3). When rats were administered a high-cholesterol diet (HCD) containing 5% LF, LF was detected using ELISA in the upper small intestine from 7.5 to 60 min after the administration. Rats were fed one of the following diets: control, HCD, or HCD + 5% LF for 21 days. Fecal neutral steroids and hepatic cholesterol levels in the HCD group were significantly higher than those in the control group. The addition of LF to a HCD significantly increased fecal neutral steroids levels (22% increase, p cholesterol levels (17% decrease, p cholesterol excretion via interactions with bile acids.

  19. Binding and Endocytosis of Bovine Hololactoferrin by the Parasite Entamoeba histolytica

    Directory of Open Access Journals (Sweden)

    Guillermo Ortíz-Estrada

    2015-01-01

    Full Text Available Entamoeba histolytica is a human parasite that requires iron (Fe for its metabolic function and virulence. Bovine lactoferrin (B-Lf and its peptides can be found in the digestive tract after dairy products are ingested. The aim of this study was to compare virulent trophozoites recently isolated from hamster liver abscesses with nonvirulent trophozoites maintained for more than 30 years in cultures in vitro regarding their interaction with iron-charged B-Lf (B-holo-Lf. We performed growth kinetics analyses of trophozoites in B-holo-Lf and throughout several consecutive transfers. The virulent parasites showed higher growth and tolerance to iron than nonvirulent parasites. Both amoeba variants specifically bound B-holo-Lf with a similar Kd. However, averages of 9.45 × 105 and 6.65 × 106 binding sites/cell were found for B-holo-Lf in nonvirulent and virulent amoebae, respectively. Virulent amoebae bound more efficiently to human and bovine holo-Lf, human holo-transferrin, and human and bovine hemoglobin than nonvirulent amoebae. Virulent amoebae showed two types of B-holo-Lf binding proteins. Although both amoebae endocytosed this glycoprotein through clathrin-coated vesicles, the virulent amoebae also endocytosed B-holo-Lf through a cholesterol-dependent mechanism. Both amoeba variants secreted cysteine proteases cleaving B-holo-Lf. These data demonstrate that the B-Lf endocytosis is more efficient in virulent amoebae.

  20. Immunomodulatory effects of recombinant lactoferrin during MRSA infection.

    Science.gov (United States)

    Hwang, Shen-An; Kruzel, Marian L; Actor, Jeffrey K

    2014-05-01

    Methicillin-resistant Staphylococcus aureus (MRSA) infection remains a serious hazard to global health. The use of immune modulatory therapy to combat infection is gaining an interest as a novel treatment alternative. Lactoferrin (LF), an iron binding protein with immune modulating properties, has the potential to modify the course of systemic MRSA infection. Specifically, LF is capable of limiting deleterious inflammatory responses while promoting the development of antigen specific T-cell activity. The efficacy of a novel recombinant mouse LF (rmLF) to protect against MRSA infection was examined in a mouse peritonitis model. BALB/c mice were infected with a lethal dose of MRSA and treated at 2h post-infection with rmLF. Effects of rmLF on MRSA-infected primary monocytes and granulocytes were analyzed for inflammatory mediators. The rmLF treated mice demonstrated a modest increase in survival of more than 24h, albeit with reduced bacteremia. Serum cytokines, IL-17 and IL-6, were significantly reduced post-challenge post-rmLF treatment. The rmLF led to a minor decrease in IL-1b, and a slight increase in TNF-a production. Preliminary investigation towards human clinical relevance was accomplished using human blood derived monocytes and granulocytes infected with MRSA and treated with homologous recombinant human LF (rhLF). Treatment with (rhLF) led to increased production of IFN-g and IL-2. The human cell studies also showed a concurrent decrease in TNF-a, IL-6, IL-1b, IL-12p40, and IL-10. These results indicate that the rmLF and rhLF have a high degree of overlap to modify inflammatory responses, although differences in activities were observed between the two heterologous recombinant molecules.

  1. Occurrence of fig wasps (Hymenoptera: Chalcidoidea) in Ficus caria and F. microcarpa in Hatay, Turkey

    OpenAIRE

    Doğanlar, Mikdat

    2012-01-01

    This study focuses on fig wasps (Hymenoptera: Chalcidoidea), considering that only 2 fig wasp species, Blastophaga psenes (L.) and Philotrypesis caricae (L.) (new record) are associated with Ficus carica in Turkey. Five fig wasps species, namely Eupristina verticillata Waterston, Walkerella microcarpae Boucek, Odontofroggatia ishii Wiebes, Philotrypesis taiwanensis Chen, and Philotrypesis emeryi Grandi were found on Ficus microcarpa (L.), which is an Asian fig tree, and has been ornamentally ...

  2. Comparisons of host mitochondrial, nuclear and endosymbiont bacterial genes reveal cryptic fig wasp species and the effects of Wolbachia on host mtDNA evolution and diversity

    Directory of Open Access Journals (Sweden)

    Feng Gui

    2011-04-01

    Full Text Available Abstract Background Figs and fig-pollinating wasp species usually display a highly specific one-to-one association. However, more and more studies have revealed that the "one-to-one" rule has been broken. Co-pollinators have been reported, but we do not yet know how they evolve. They may evolve from insect speciation induced or facilitated by Wolbachia which can manipulate host reproduction and induce reproductive isolation. In addition, Wolbachia can affect host mitochondrial DNA evolution, because of the linkage between Wolbachia and associated mitochondrial haplotypes, and thus confound host phylogeny based on mtDNA. Previous research has shown that fig wasps have the highest incidence of Wolbachia infection in all insect taxa, and Wolbachia may have great influence on fig wasp biology. Therefore, we look forward to understanding the influence of Wolbachia on mitochondrial DNA evolution and speciation in fig wasps. Results We surveyed 76 pollinator wasp specimens from nine Ficus microcarpa trees each growing at a different location in Hainan and Fujian Provinces, China. We found that all wasps were morphologically identified as Eupristina verticillata, but diverged into three clades with 4.22-5.28% mtDNA divergence and 2.29-20.72% nuclear gene divergence. We also found very strong concordance between E. verticillata clades and Wolbachia infection status, and the predicted effects of Wolbachia on both mtDNA diversity and evolution by decreasing mitochondrial haplotypes. Conclusions Our study reveals that the pollinating wasp E. verticillata on F. microcarpa has diverged into three cryptic species, and Wolbachia may have a role in this divergence. The results also indicate that Wolbachia strains infecting E. verticillata have likely resulted in selective sweeps on host mitochondrial DNA.

  3. Occurrence of fig wasps (Hymenoptera: Chalcidoidea) in Ficus caria and F. microcarpa in Hatay, Turkey

    OpenAIRE

    Doğanlar, Mikdat

    2014-01-01

    This study focuses on fig wasps (Hymenoptera: Chalcidoidea), considering that only 2 fig wasp species, Blastophaga psenes (L.) and Philotrypesis caricae (L.) (new record) are associated with Ficus carica in Turkey. Five fig wasps species, namely Eupristina verticillata Waterston, Walkerella microcarpae Boucek, Odontofroggatia ishii Wiebes, Philotrypesis taiwanensis Chen, and Philotrypesis emeryi Grandi were found on Ficus microcarpa (L.), which is an Asian fig tree, and has been ornamentally ...

  4. New occurrence of non-pollinating fig wasps (Hymenoptera: Chalcidoidea) in Ficus microcarpa in Brazil

    OpenAIRE

    Farache, Fernando H.A.; Ó,Vanessa T. do; Rodrigo A. S. Pereira

    2009-01-01

    Ficus microcarpa is an Asian fig tree that is ornamentally cultivated. The specific pollinator, Eupristina verticillata Waterston, and the non-pollinators Walkerella microcarpae Bouček and Philotrypesis emeryi Grandi, have been reported associated to F. microcarpa in Brazil. In here we report for the first time the occurrence of Odontofroggatia ishii Wiebes and Philotrypesis taiwanensis Chen et al in F. microcarpa in Brazil. Our results suggest that P. taiwanensis and O. ishii represent ...

  5. Environ: E00144 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available E00144 Fritillaria bulb (JP16) Crude drug Peimine [CPD:C10830], Peiminine [CPD:C108...08], Zhebeinine [CPD:C17881], Fritillarine [CPD:C10808], Peiminoside [CPD:C17882], (Fritilline | Verticillin...e), Apoverticine, Peimiphine, Peimidine, Peimitidine, Peiminoside, Fritimine Fritillaria verticillata [TAX:5...9070] Same as: D06774 Liliaceae (lily family) Fritillaria bulb Major component: P

  6. Caulerpa (Chlorophyta, Caulerpales) from the Kenyan coast

    OpenAIRE

    Coppejans, E.; Beeckman, T.

    1990-01-01

    In view of preparing a Flora of the Seaweeds from Kenya, material is collected yearly by the authors since 1985. Twelve species of Caulerpa have been collected so far, some including several varieties: C. brachypus, C. cupressoides, C. elongata, C. fastigiata, C. lentillifera, C. mexicana, C. racemosa (with several var.), C. scalpelliformis, C. serrulata, C. sertularioides, C. taxifolia, C. verticillata. An identification key is provided, the different species are described and illustrated (e...

  7. Marine algae of Papua New Guinea (Madang Prov.) 2. A revised and completed list of Caulerpa (Chlorophyta—Caulerpales)

    OpenAIRE

    Coppejans, E.

    1992-01-01

    The study of marine macroalgae collected in N Papua New Guinea in 1980, 1986, 1988 and 1990 resulted in 14 species (29 entities) of Caulerpa: C. biserrulata, C. cupressoides (5 ecads), c. elongata (2 ecads), C. filicoides var. andamanensis, C. lentillifera, C. manorensis, C. microphysa, C. opposita, C. racemosa (8 ecads), C. serrulata (3 ecads), C. sertularioides, C. taxifolia (2 ecads), C. verticillata, and C. webbiana ecad disticha. An identification key is added.

  8. Ash characteristics and plant nutrients in some aquatic biomasses

    Science.gov (United States)

    Masto, Reginald; Pandit, Ankita; George, Joshy; Mukhopadhyay, Sangeeta; Selvi, Vetrivel; Ram, Lal

    2016-04-01

    Aquatic biomasses are explored as potential fuel source for direct combustion because of their faster growth and no land requirement. The energy density and the ash characteristics of the aquatic biomasses are to be evaluated for their suitability for energy extraction. In the study, four aquatic plant samples namely Eichornia crassipes, Hydrilla verticilleta, Lemna minor, Spirogyra spp were collected from a pond in Digwadih Campus of Central Institute of Mining and Fuel Research, Dhanbad. The biomasses were air dried, powdered and ashed at different temperatures. Volatile C was relatively lower in Spirogyra and Hydrilla (53 %) than Eichornia (62.6 %) or Lemna (59.7 %), whereas fixed C was higher for Eichornia and Lemna (about 10 %) and lower for Hydrilla (1 %). Ultimate analysis showed that the carbon content was in the order Eichornia > Lemna > Spirogyra > Hydrilla. The IR spectra of each raw biomass is compared to their respective ashes obtained at different temperatures (500-900°C). With increase in ashing temperature from 500-900°C there is gradual breakdown of the cellulosic structure hence, peaks around 2900-2800cm-1 caused by aliphatic C-H vibration tends to disappear slowly in ash. More number of peaks appears at lower wavenumbers in ashes of all the biomass samples indicating towards increased percentage of inorganic ion species. Considerable enrichment of SiO2 is validated with prominent peaks at 1100-900 cm-1 in all the ashes. Lemna and Spirogyra has a similar ash composition (Si > Al > Ca > K), whereas, Ca was higher in Hydrilla (Si > Ca > K > Al). Eichornia (Si > K > Ca > Al) has higher K and Ca than Al. SiO2 and Al2O3 were higher in Spirogyra, while SiO2 and CaO in Eichornia and Hydrilla. K first increased from 500-700/800⁰C, and then decreased from 800-900⁰C. Cl is lost slowly in ash from 500-700/800⁰C and then by a drastic reduction from 800-900⁰C. S is enhanced in ash at all temperatures although the change is quite small. Most of the Cl

  9. Lactoferrin, myeloperoxidase, and ceruloplasmin: complementary gearwheels cranking physiological and pathological processes.

    Science.gov (United States)

    Sokolov, Alexey V; Zakharova, Elena T; Zakahrova, Elena T; Kostevich, Valeria A; Samygina, Valeria R; Vasilyev, Vadim B

    2014-10-01

    Copper-containing plasma protein ceruloplasmin (Cp) forms a complex with lactoferrin (Lf), an iron-binding protein, and with the heme-containing myeloperoxidase (Mpo). In case of inflammation, Lf and Mpo are secreted from neutrophil granules. Among the plasma proteins, Cp seems to be the preferential partner of Lf and Mpo. After an intraperitoneal injection of Lf to rodents, the "Cp-Lf" complex has been shown to appear in their bloodstream. Cp prevents the interaction of Lf with protoplasts of Micrococcus luteus. Upon immunoprecipitation of Cp, the blood plasma becomes depleted of Lf and in a dose-dependent manner loses the capacity to inhibit the peroxidase activity of Mpo, but not the Mpo-catalyzed oxidation of thiocyanate in the (pseudo)halogenating cycle. Antimicrobial effect against E. coli displayed by a synergistic system that includes Lf and Mpo-H2O2-chloride, but not thiocyanate, as the substrate for Mpo is abrogated when Cp is added. Hence, Cp can be regarded as an anti-inflammatory factor that restrains the halogenating cycle and redirects the synergistic system Mpo-H2O2-chloride/thiocyanate to production of hypothiocyanate, which is relatively harmless for the human organism. Structure and functions of the "2Cp-2Lf-Mpo" complex and binary complexes Cp-Lf and 2Cp-Mpo in inflammation are discussed.

  10. Bovine lactoferrin decreases cholera-toxin-induced intestinal fluid accumulation in mice by ganglioside interaction.

    Directory of Open Access Journals (Sweden)

    Fulton P Rivera

    Full Text Available Secretory diarrhea caused by cholera toxin (CT is initiated by binding of CT's B subunit (CTB to GM1-ganglioside on the surface of intestinal cells. Lactoferrin, a breast milk glycoprotein, has shown protective effect against several enteropathogens. The aims of this study were to determine the effect of bovine-lactoferrin (bLF on CT-induced intestinal fluid accumulation in mice, and the interaction between bLF and CT/CTB with the GM1-ganglioside receptor. Fluid accumulation induced by CT was evaluated in the mouse ileal loop model using 56 BALB/c mice, with and without bLF added before, after or at the same time of CT administration. The effect of bLF in the interaction of CT and CTB with GM1-ganglioside was evaluated by a GM1-enzyme-linked immunosorbent assay. bLF decreased CT-induced fluid accumulation in the ileal loop of mice. The greatest effect was when bLF was added before CT (median, 0.066 vs. 0.166 g/cm, with and without bLF respectively, p<0.01. We conclude that bLF decreases binding of CT and CTB to GM1-ganglioside, suggesting that bLF suppresses CT-induced fluid accumulation by blocking the binding of CTB to GM1-ganglioside. bLF may be effective as adjunctive therapy for treatment of cholera diarrhea.

  11. Bovine Lactoferrin Decreases Cholera-Toxin-Induced Intestinal Fluid Accumulation in Mice by Ganglioside Interaction

    Science.gov (United States)

    Rivera, Fulton P.; Medina, Anicia M.; Bezada, Sandra; Valencia, Roberto; Bernal, María; Meza, Rina; Maves, Ryan C.; Ochoa, Theresa J.

    2013-01-01

    Secretory diarrhea caused by cholera toxin (CT) is initiated by binding of CT’s B subunit (CTB) to GM1-ganglioside on the surface of intestinal cells. Lactoferrin, a breast milk glycoprotein, has shown protective effect against several enteropathogens. The aims of this study were to determine the effect of bovine-lactoferrin (bLF) on CT-induced intestinal fluid accumulation in mice, and the interaction between bLF and CT/CTB with the GM1-ganglioside receptor. Fluid accumulation induced by CT was evaluated in the mouse ileal loop model using 56 BALB/c mice, with and without bLF added before, after or at the same time of CT administration. The effect of bLF in the interaction of CT and CTB with GM1-ganglioside was evaluated by a GM1-enzyme-linked immunosorbent assay. bLF decreased CT-induced fluid accumulation in the ileal loop of mice. The greatest effect was when bLF was added before CT (median, 0.066 vs. 0.166 g/cm, with and without bLF respectively, p<0.01). We conclude that bLF decreases binding of CT and CTB to GM1-ganglioside, suggesting that bLF suppresses CT-induced fluid accumulation by blocking the binding of CTB to GM1-ganglioside. bLF may be effective as adjunctive therapy for treatment of cholera diarrhea. PMID:23580005

  12. In silico investigation of lactoferrin protein characterizations for the prediction of anti-microbial properties

    Science.gov (United States)

    Sohrabi, Seyyed Mohsen; Niazi, Ali; Chahardoli, Mahmood; Hortamani, Ali; Setoodeh, Payam

    2014-01-01

    Lactoferrin (Lf) is an iron-binding multi-functional glycoprotein which has numerous physiological functions such as iron transportation, anti-microbial activity and immune response. In this study, different in silico approaches were exploited to investigate Lf protein properties in a number of mammalian species. Results showed that the iron-binding site, DNA and RNA-binding sites, signal peptides and transferrin motifs in the Lf structure were highly conserved. Examined sequences showed three conserved motifs which were repeated twice in the Lf structure, demonstrating ancient duplication events in its gene. Also, results suggest that the functional domains in mammalian Lf proteins are Zinc finger, Tubulin/FtsZ, GTPase, α/β hydrolase and Zinc knuckle. The potential site for nucleic acid binding and the major DNA and RNA- binding sites in this protein were found in the lactoferricin (Lfc) fragment. Due to its high positive charge, Lf is able to bind a large number of compounds. Our analysis also revealed that the interactions between Lf and ITLN1, LYZ, CSN2, and CD14 proteins played an important role in the protective activities of Lf. Analysis for the prediction of secondary structures indicated that high amounts of α-helix, β-strand and β-sheet were present in Lf. The high degree of conservation among mammalian Lf proteins indicates that there is a close relationship between these proteins, reflecting their important role. PMID:27843978

  13. Immunolocalization of lactoferrin in surgically resected pigmented skin lesions

    Directory of Open Access Journals (Sweden)

    G Tuccari

    2009-06-01

    Full Text Available Lactoferrin (Lf expression was determined immunohistochemically in 57 formalin-fixed paraffin-embedded bioptic samples obtained from an equal number of patients treated by surgery to remove pigmented skin lesions (nevi = 23; melanoma = 12; vulgaris and seborrhoeic warts = 12; basal cell carcinoma = 10; in addition, 10 specimens of normal skin were studied as control. On 3 ?m thick sections, depigmentation and antigen retrieval procedures were performed. The Lf immunoreactivity was revealed by a rabbit anti-human Lf. Quantification of Lf immunoreactivity was performed using an intensity-distribution (ID score. Melanocytic cells, regardless of their benign or malignant nature, were consistently stained, with no significant differences in the Lf IDscore between melanomas or nevi. A different intensity of Lf immunoreactivity was encountered in superficial portions of warts, exclusively inside squamous epithelial cells arranged in sheets or whorls of keratin. On the contrary, basal cell carcinomas were always unstained, while a slight Lf positivity was found in focal keratinized areas present in two tumours showing baso-squamous differentiation. The Lf immunoreactivity was localized in the cytoplasm and only occasionally in the nucleus. The biological meaning of Lf in these cases of human skin specimens remains unexplained, although it cannot be ruled out that Lf might be involved in the defense system against tumours, or alternatively, may be used by cells requiring iron availability for their turnover. Moreover, the immunohistochemical expression of Lf in melanocytic lesions might be also related to a Lf-melanin interaction. Finally, the involvement of Lf in skin squamous non-neoplastic elements could be related to its role as one of the molecules modulating an unspecific inflammatory or anti-oxidant response.

  14. Experimental Mouse Model of Lumbar Ligamentum Flavum Hypertrophy.

    Science.gov (United States)

    Saito, Takeyuki; Yokota, Kazuya; Kobayakawa, Kazu; Hara, Masamitsu; Kubota, Kensuke; Harimaya, Katsumi; Kawaguchi, Kenichi; Hayashida, Mitsumasa; Matsumoto, Yoshihiro; Doi, Toshio; Shiba, Keiichiro; Nakashima, Yasuharu; Okada, Seiji

    2017-01-01

    Lumbar spinal canal stenosis (LSCS) is one of the most common spinal disorders in elderly people, with the number of LSCS patients increasing due to the aging of the population. The ligamentum flavum (LF) is a spinal ligament located in the interior of the vertebral canal, and hypertrophy of the LF, which causes the direct compression of the nerve roots and/or cauda equine, is a major cause of LSCS. Although there have been previous studies on LF hypertrophy, its pathomechanism remains unclear. The purpose of this study is to establish a relevant mouse model of LF hypertrophy and to examine disease-related factors. First, we focused on mechanical stress and developed a loading device for applying consecutive mechanical flexion-extension stress to the mouse LF. After 12 weeks of mechanical stress loading, we found that the LF thickness in the stress group was significantly increased in comparison to the control group. In addition, there were significant increases in the area of collagen fibers, the number of LF cells, and the gene expression of several fibrosis-related factors. However, in this mecnanical stress model, there was no macrophage infiltration, angiogenesis, or increase in the expression of transforming growth factor-β1 (TGF-β1), which are characteristic features of LF hypertrophy in LSCS patients. We therefore examined the influence of infiltrating macrophages on LF hypertrophy. After inducing macrophage infiltration by micro-injury to the mouse LF, we found excessive collagen synthesis in the injured site with the increased TGF-β1 expression at 2 weeks after injury, and further confirmed LF hypertrophy at 6 weeks after injury. Our findings demonstrate that mechanical stress is a causative factor for LF hypertrophy and strongly suggest the importance of macrophage infiltration in the progression of LF hypertrophy via the stimulation of collagen production.

  15. Biological screening of rain forest plot trees from Palawan Island (Philippines).

    Science.gov (United States)

    Horgen, F D; Edrada, R A; de los Reyes, G; Agcaoili, F; Madulid, D A; Wongpanich, V; Angerhofer, C K; Pezzuto, J M; Soejarto, D D; Farnsworth, N R

    2001-01-01

    Study plots totaling 0.2 Ha were established in primary forest in the highlands of central Palawan Island, Philippines. Samples of various anatomical parts [typically leaf + twig (If/tw), stem bark (sb), and root (rt)] were collected from all tree species represented within the plots by individuals having a diameter at breast height > or = 10 cm. In all, 211 distinct samples were obtained from 68 tree species, representing 35 families (not including samples from 4 indeterminate species). Methanol extracts of these samples were tested in in vitro antiplasmodial, brine shrimp toxicity, and cytotoxicity assays. The following samples showed an IC50 Meliosma pinnata ssp. macrophylla (lf/tw, rt), Myristica guatteriifolia (lf/tw), Ochrosia glomerata (rt, sb), Swintonia foxworthyi (lf/tw), Syzygium sp. 1 (rt), Turpinia pomifera (rt), and Xanthophyllum flavescens (sb). Secondly, those samples which displayed > or = 50% immobilization of brine shrimp at 100 microg/mL were: Acronychia laurifolia (lf/tw/fruit, rt, sb), Agathis celebica (lf/tw, sb), Aglaia sp. 1 (lf/tw), Alphonsea sp. 1 (rt), Ardisia iwahigensis (lf/tw), Arthrophyllum ahernianum (lf/tw, rt, sb), Castanopsis cf. evansii (rt), Cinnamomum griffithii (lf/tw, rt), Croton argyratus (lf/tw), C. leiophyllus (lf/tw, rt), Dysoxylum cauliflorum (fruit, lf/tw, rt), Euonymus javanicus (rt), Glochidion sp. 1 (rt), Polyosma sp. 1 (rt), Symplocos polyandra (rt), Timonius gammillii (sb), and Xanthophyllum flavescens (rt). Lastly, samples which exhibited an IC50 < or = 20 microg/mL against one or more of the cancer cell lines employed (LU1, KB, KB-V1, P-388, LNCaP, or ZR-75-1) include: Acronychia laurifolia (lf/tw/fruit, rt, sb), Aglaia sp. 1 (sb), Aglaia sp. 2 (rt), Alphonsea sp. 1 (rt), Ardisia iwahigensis (lf/tw, rt, sb), Astronia cumingiana (sb), Croton argyratus (lf/tw, rt, sb), C. leiophyllus (lf/tw, rt), Dimorphocalyx murina (lf/tw, rt, sb), Lithocarpus caudatifolius (rt, sb), Litsea cf. sibuyanensis (rt), Syzygium cf

  16. Low-Frequency Electromagnetic Field Exposure Enhances Extracellular Trap Formation by Human Neutrophils through the NADPH Pathway.

    Science.gov (United States)

    Golbach, Lieke A; Scheer, Marleen H; Cuppen, Jan J M; Savelkoul, Huub; Verburg-van Kemenade, B M Lidy

    2015-01-01

    Low-frequency (LF) electromagnetic fields (EMFs) are abundantly present in modern society, and the potential biological consequences of exposure to these fields are under intense debate. Immune cells are suggested as possible target cells, though a clear mechanism is lacking. Considering their crucial role in innate immune activation, we selected an ex vivo exposure set-up with human neutrophils to investigate a possible correlation between neutrophil extracellular trap (NET) formation and LF EMF exposure. Our study shows that formation of NETs is enhanced by LF EMF exposure. Enhanced NET formation leads to increased antimicrobial properties as well as damage to surrounding cells. We found that LF-EMF-induced NET formation is dependent on the NADPH oxidase pathway and production of reactive oxygen species. Additionally, LF EMF exposure does not influence autophagy and PAD4 activity. Our study provides a mechanism by which exposure to LF EMFs could influence the innate immune system.

  17. Light-Front quantization of field theory

    CERN Document Server

    Srivastava, P P

    1996-01-01

    Some basic topics in Light-Front (LF) quantized field theory are reviewed. Poincarè algebra and the LF Spin operator are discussed. The local scalar field theory of the conventional framework is shown to correspond to a non-local Hamiltonian theory on the LF in view of the constraint equations on the phase space, which relate the bosonic condensates to the non-zero modes. This new ingredient is useful to describe the spontaneous symmetry breaking on the LF. The instability of the symmetric phase in two dimensional scalar theory when the coupling constant grows is shown in the LF theory renormalized to one loop order. Chern-Simons gauge theory regarded to describe excitations with fractional statistics, is quantized in the light-cone gauge and a simple LF Hamiltonian obtained which may allow us to construct renormalized theory of anyons.

  18. High-Level Expression of Recombinant Bovine Lactoferrin in Pichia pastoris with Antimicrobial Activity

    Science.gov (United States)

    Iglesias-Figueroa, Blanca; Valdiviezo-Godina, Norberto; Siqueiros-Cendón, Tania; Sinagawa-García, Sugey; Arévalo-Gallegos, Sigifredo; Rascón-Cruz, Quintín

    2016-01-01

    In this study, bovine lactoferrin (bLf), an iron-binding glycoprotein considered an important nutraceutical protein because of its several properties, was expressed in Pichia pastoris KM71-H under AOX1 promoter control, using pJ902 as the recombinant plasmid. Dot blotting analysis revealed the expression of recombinant bovine lactoferrin (rbLf) in Pichia pastoris. After Bach fermentation and purification by molecular exclusion, we obtained an expression yield of 3.5 g/L of rbLf. rbLf and predominantly pepsin-digested rbLf (rbLfcin) demonstrated antibacterial activity against Escherichia coli (E. coli) BL21DE3, Staphylococcus aureus (S. aureus) FRI137, and, in a smaller percentage, Pseudomonas aeruginosa (Ps. Aeruginosa) ATCC 27833. The successful expression and characterization of functional rbLf expressed in Pichia pastoris opens a prospect for the development of natural antimicrobial agents produced recombinantly. PMID:27294912

  19. High-Level Expression of Recombinant Bovine Lactoferrin in Pichia pastoris with Antimicrobial Activity

    Directory of Open Access Journals (Sweden)

    Blanca Iglesias-Figueroa

    2016-06-01

    Full Text Available In this study, bovine lactoferrin (bLf, an iron-binding glycoprotein considered an important nutraceutical protein because of its several properties, was expressed in Pichia pastoris KM71-H under AOX1 promoter control, using pJ902 as the recombinant plasmid. Dot blotting analysis revealed the expression of recombinant bovine lactoferrin (rbLf in Pichia pastoris. After Bach fermentation and purification by molecular exclusion, we obtained an expression yield of 3.5 g/L of rbLf. rbLf and predominantly pepsin-digested rbLf (rbLfcin demonstrated antibacterial activity against Escherichia coli (E. coli BL21DE3, Staphylococcus aureus (S. aureus FRI137, and, in a smaller percentage, Pseudomonas aeruginosa (Ps. Aeruginosa ATCC 27833. The successful expression and characterization of functional rbLf expressed in Pichia pastoris opens a prospect for the development of natural antimicrobial agents produced recombinantly.

  20. Mapping the epitopes of a neutralizing antibody fragment directed against the lethal factor of Bacillus anthracis and cross-reacting with the homologous edema factor.

    Directory of Open Access Journals (Sweden)

    Philippe Thullier

    Full Text Available The lethal toxin (LT of Bacillus anthracis, composed of the protective antigen (PA and the lethal factor (LF, plays an essential role in anthrax pathogenesis. PA also interacts with the edema factor (EF, 20% identity with LF to form the edema toxin (ET, which has a lesser role in anthrax pathogenesis. The first recombinant antibody fragment directed against LF was scFv 2LF; it neutralizes LT by blocking the interaction between PA and LF. Here, we report that scFv 2LF cross-reacts with EF and cross-neutralizes ET, and we present an in silico method taking advantage of this cross-reactivity to map the epitope of scFv 2LF on both LF and EF. This method identified five epitope candidates on LF, constituted of a total of 32 residues, which were tested experimentally by mutating the residues to alanine. This combined approach precisely identified the epitope of scFv 2LF on LF as five residues (H229, R230, Q234, L235 and Y236, of which three were missed by the consensus epitope candidate identified by pre-existing in silico methods. The homolog of this epitope on EF (H253, R254, E258, L259 and Y260 was experimentally confirmed to constitute the epitope of scFv 2LF on EF. Other inhibitors, including synthetic molecules, could be used to target these epitopes for therapeutic purposes. The in silico method presented here may be of more general interest.

  1. Lymphatic filariasis in Nigeria; micro-stratification overlap mapping (MOM as a prerequisite for cost-effective resource utilization in control and surveillance.

    Directory of Open Access Journals (Sweden)

    Patricia N Okorie

    Full Text Available BACKGROUND: Nigeria has a significant burden of lymphatic filariasis (LF caused by the parasite Wuchereria bancrofti. A major concern to the expansion of the LF elimination programme is the risk of serious adverse events (SAEs associated with the use of ivermectin in areas co-endemic with Loa filariasis. To better understand this, as well as other factors that may impact on LF elimination, we used Micro-stratification Overlap Mapping (MOM to highlight the distribution and potential impact of multiple disease interventions that geographically coincide in LF endemic areas and which will impact on LF and vice versa. METHODOLOGY/PRINCIPAL FINDINGS: LF data from the literature and Federal Ministry of Health (FMoH were collated into a database. LF prevalence distributions; predicted prevalence of loiasis; ongoing onchocerciasis community-directed treatment with ivermectin (CDTi; and long-lasting insecticidal mosquito net (LLIN distributions for malaria were incorporated into overlay maps using geographical information system (GIS software. LF was prevalent across most regions of the country. The mean prevalence determined by circulating filarial antigen (CFA was 14.0% (n = 134 locations, and by microfilaria (Mf was 8.2% (n = 162 locations. Overall, LF endemic areas geographically coincided with CDTi priority areas, however, LLIN coverage was generally low (<50% in areas where LF prevalence was high or co-endemic with L. loa. CONCLUSIONS/SIGNIFICANCE: The extensive database and series of maps produced in this study provide an important overview for the LF Programme and will assist to maximize existing interventions, ensuring cost effective use of resources as the programme scales up. Such information is a prerequisite for the LF programme, and will allow for other factors to be included into planning, as well as monitoring and evaluation activities given the broad spectrum impact of the drugs used.

  2. Dicty_cDB: VSB776 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 1085 Drought Medicago truncatula cDNA clone NF064C11DT 5', mRNA sequence. 90 5e-14 1 AW682908 |AW682908.2 NF001G03LF1F1023 Developing...430 |AW683430.2 NF011H04LF1F1043 Developing leaf Medicago truncatula cDNA clone NF011H04LF 5', mRNA sequence

  3. Calculating sympathovagal balance from heart rate variability: are there alternatives in adolescents?

    Science.gov (United States)

    Dalla Pozza, Robert; Kleinmann, Arne; Bechtold, Susanne; Kozlik-Feldmann, R; Daebritz, S; Netz, Heinrich

    2006-06-01

    Assessing sympathovagal balance by calculating LF/HF-ratio from power spectral analysis (PSA) of heart rate variability (HRV) may be difficult in adolescents as chaotic breathing leads to methodical bias and metronomic breathing is not easy to perform. Diastolic blood pressure variability (dBPV) is less influenced and may therefore offer more stable values for calculations. The present study was performed on 72 paediatric subjects to investigate possible alternative LF/HF-calculations from PSA of HRV and dBPV. Seventy-two paediatric individuals in three groups: 12 controls, 17 heart- and heart-lung-transplanted children (TX) and 43 adolescents born small for gestational age (SGA). Short-term beat-to-beat HRV and BP-recordings were made supine and during active standing. Ratios calculated: LF/HF from HRV, LF/HF from dBPV, LF-dBPV/HF-HRV and LF-HRV/HF-dBPV. LF/HF from dBPV as well as LF-HRV/HF-dBPV did not correlate with LF/HF-HRV. Correlation of LF/HF from HRV and LF-dBPV/HF-HRV was high especially in TX and in patients with resting heart rate of above 90 beats per minute. In adolescents, the ratio of LF-dBPV/HF-HRV may be an alternative method for calculating sympathicovagal balance being less influenced by breathing patterns. In younger patients with elevated resting heart rate, but also in patients with very low HRV such as TX-patients this method could be a supplemental diagnostic tool whenever autonomic nervous control on the cardiocirculatory system has to be assessed.

  4. Blocking anthrax lethal toxin at the protective antigen channel by using structure-inspired drug design

    OpenAIRE

    Karginov, Vladimir A.; Nestorovich, Ekaterina M.; Moayeri, Mahtab; Leppla, Stephen H.; Bezrukov, Sergey M.

    2005-01-01

    Bacillus anthracis secretes three polypeptides: protective antigen (PA), lethal factor (LF), and edema factor (EF), which interact at the surface of mammalian cells to form toxic complexes. LF and EF are enzymes that target substrates within the cytosol; PA provides a heptameric pore to facilitate LF and EF transport into the cytosol. Other than administration of antibiotics shortly after exposure, there is currently no approved effective treatment for inhalational anthrax. Here we demonstrat...

  5. Genetic variability of lactoferrin content estimated by mid-infrared spectrometry in bovine milk

    OpenAIRE

    Soyeurt, Hélène; Colinet, Frédéric; Arnould, Valérie; Dardenne, Pierre; Bertozzi, Carlo; Renaville, Robert; Portetelle, Daniel; Gengler, Nicolas

    2007-01-01

    The effects of lactoferrin (LF) on the immune system have already been shown by many studies. Unfortunately, the current methods used to measure LF levels in milk do not permit the study of the genetic variability of lactoferrin or the performance of routine genetic evaluations. The first aim of this research was to derive a calibration equation permitting the prediction of LF in milk by mid-infrared spectrometry (MIR). The calibration with partial least squares on 69 ...

  6. Bacterial cellulose-lactoferrin as an antimicrobial edible packaging

    OpenAIRE

    Padrão, Jorge; S. Gonçalves; Silva, João P.; Sencadas, Vítor João Gomes Silva; Lanceros-Méndez, S.; A. C. Pinheiro; Vicente, A.A.; RODRIGUES, L. R.; Dourado, Fernando

    2016-01-01

    Bacterial cellulose (BC) films from two distinct sources (obtained by static culture with Gluconacetobacter xylinus ATCC 53582 (BC1) and from a commercial source (BC2)) were modified by bovine lactoferrin (bLF) adsorption. The functionalized films (BC+bLF) were assessed as edible antimicrobial packaging, for use in direct contact with highly perishable foods, specifically fresh sausage as a model of meat products. BC+bLF films and sausage casings were characterized regarding their water vapou...

  7. Design and evaluation of Lumefantrine – Oleic Acid Self Nanoemulsifying Ionic Complex for Enhanced Dissolution

    Directory of Open Access Journals (Sweden)

    Pradeep Vavia

    2013-03-01

    Full Text Available Background:Lumefantrine, an antimalarial molecule has very low and variable bioavailability owing to its extremely poor solubility in water. It is recommended to be taken with milk to enhance its solubility and bioavailability. The aim of present study was to develop a Self Nanoemulsifying Delivery system (SNEDs of lumefantrine (LF to achieve rapid and complete dissolution independent of food-fat and surfactant in dissolution media.Methods:Solubility of LF in oil, co-solvent/co-surfactant and surfactant solution and emulsification efficiency of surfactant were analyzed to optimize the LF loaded self nanoemulsifying preconcentrate. Effect of LF-oleic acid complexation on emulsification, droplet size, zeta potential and dissolution were investigated. Effect of milk concentration and fat content on saturation solubility and dissolution of LF was investigated. Dissolution of marketed formulation and LF-SNEDs was carried out in pH 1.2 and pH 6.8 phosphate buffer.Results:LF exhibited very high solubility in oleic acid owing to complexation between tertiary amine of LF and carboxyl group of oleic acid (OA. Cremophore EL and medium chain monoglyceride were selected surfactant and co-surfactant, respectively. Significantly smaller droplet size (37 nm, shift in zeta potential from negative to positive value, very high drug loading in lipid based system (> 10%, no precipitation after dissolution are the major distinguish characteristics contributed by LF-OA complex in the SNED system. Saturation solubility and dissolution study in milk containing media pointed the significant increment in solubility of LF in the presence of milk-food fat. LF-SNEDs showed > 90% LF release within 30 min in pH 1.2 while marketed tablet showed almost 0% drug release.Conclusion:Self nanoemulsification promoting ionic complexation between basic drug and oleic acid hold great promise in enhancing solubility of hydrophobic drugs.

  8. Long-term effects of psychosocial factors of home and work on biomarkers of stress

    DEFF Research Database (Denmark)

    Eller, Nanna Hurwitz; Kristiansen, Jesper; Hansen, Åse Marie

    2011-01-01

    LF/HF. In work, lack of control was associated with low LnTP, and lack of support was associated with an increased LnLF/HF ratio. For men, high social status was associated with low LnTP, low LnHF and high LnCortisol. Greater number of hours spent doing housework was associated with both low LnLF/HF and low Ln...

  9. Lactoferrin-modified PEGylated liposomes loaded with doxorubicin for targeting delivery to hepatocellular carcinoma.

    Science.gov (United States)

    Wei, Minyan; Guo, Xiucai; Tu, Liuxiao; Zou, Qi; Li, Qi; Tang, Chenyi; Chen, Bao; Xu, Yuehong; Wu, Chuanbin

    2015-01-01

    Lactoferrin (Lf) is a potential-targeting ligand for hepatocellular carcinoma (HCC) cells because of its specific binding with asialoglycoprotein receptor (ASGPR). In this present work, a doxorubicin (DOX)-loaded, Lf-modified, polyethylene glycol (PEG)ylated liposome (Lf-PLS) system was developed, and its targeting effect and antitumor efficacy to HCC was also explored. The DOX-loaded Lf-PLS system had spherical or oval vesicles, with mean particle size approximately 100 nm, and had an encapsulation efficiency of 97%. The confocal microscopy and flow cytometry indicated that the cellular uptake of Lf-PLS was significantly higher than that of PEGylated liposome (PLS) in ASGPR-positive cells (PASGPR-negative cells (P>0.05). Cytotoxicity assay by MTT demonstrated that DOX-loaded Lf-PLS showed significantly stronger antiproliferative effects on ASGPR-positive HCC cells than did PLS without the Lf modification (P<0.05). The in vivo antitumor studies on male BALB/c nude mice bearing HepG2 xenografts demonstrated that DOX-loaded Lf-PLS had significantly stronger antitumor efficacy compared with PLS (P<0.05) and free DOX (P<0.05). All these results demonstrated that a DOX-loaded Lf-PLS might have great potential application for HCC-targeting therapy.

  10. A near infrared test for two recent luminosity functions for galaxies

    CERN Document Server

    Zaninetti, L

    2014-01-01

    Two recent luminosity function (LF) for galaxies are reviewed and the parameters which characterize the near infrared are fixed. A first LF is a modified Schechter LF with four parameters. The second LF is derived from the generalized gamma and has four parameters. The formulas which give the number of galaxies as function of the redshift are reviewed and a special attention is given to the position of the photometric maximum which is expressed as function of a critical parameter or the flux of radiation or the apparent magnitude. A simulation of the 2MASS Redshift Survey is given in the framework of the non Poissonian Voronoi Tessellation.

  11. Lactoferrin targets T cells in the small intestine

    DEFF Research Database (Denmark)

    Nielsen, Sanne Mie; Hansen, Gert Helge; Danielsen, E Michael

    2010-01-01

    pathogens, and Lf receptors have been identified at the surfaces of a number of different cells. In the small intestine Lf binds to the luminal surface, but its further interaction with the epithelial cells is controversial. METHODS: In the present work, we studied the uptake of Lf in cultured mucosal...... explants of pig small intestine by immunofluorescence and immunogold microscopy. RESULTS: Lf rapidly bound to the brush border and subsequently appeared in punctae in the apical cytoplasm, indicating internalization into an endosomal compartment. Essentially, no labeling was detected elsewhere...... defense of the small intestinal mucosa by targeting the population of T cells in the lamina propria....

  12. Dicty_cDB: SSK673 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available KACGISLDSFMNTNGLNLNSCNYIQIGHDYCCN*tminkf**ylflilffs Translated Amino Acid sequence (All Frames) Frame A: kky...lf Frame B: KNTSFKIFFKKKKKKMNKLLNLLGFAVFFVLCLFSVGSNAEENGCSSWVQAREGYTCWAM SKACGISLDSFMNTNGLNLNSCNYIQIGHDYCCN*tmink

  13. Dicty_cDB: SHC556 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 2002.10.25 Translated Amino Acid sequence vkccstinptfmink*KRREKKMNVKEMDFNDLSNKSNQIINRISSFSSPIGLSSFNIER NLQEI...IVKQYHLLNNFDEFTNRSALFAGRKQTIQDLQSRTQCIALFAGKIDFPMXW*yltsfkn lf Translated Amino Acid sequence (All Frames) Frame A: vkccstinptfmink

  14. A Kinetic Ladle Furnace Process Simulation Model: Effective Equilibrium Reaction Zone Model Using FactSage Macro Processing

    Science.gov (United States)

    Van Ende, Marie-Aline; Jung, In-Ho

    2017-02-01

    The ladle furnace (LF) is widely used in the secondary steelmaking process in particular for the de-sulfurization, alloying, and reheating of liquid steel prior to the casting process. The Effective Equilibrium Reaction Zone model using the FactSage macro processing code was applied to develop a kinetic LF process model. The slag/metal interactions, flux additions to slag, various metallic additions to steel, and arcing in the LF process were taken into account to describe the variations of chemistry and temperature of steel and slag. The LF operation data for several steel grades from different plants were accurately described using the present kinetic model.

  15. Dicty_cDB: VSB543 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available LEIEGGAGANDYEEIAKVEHLTGIEIH*ed*miesikler nkvikkvvksfsvilakvqmlvlcggqsfn*lf*f*lvfgk*nilnhfsrprnlykhiil ikfk T...LSKTMPSNGRFAFSTQVGGEHK ICFSTNTSKWFGPSVKTRLHLEIEGGAGANDYEEIAKVEHLTGIEIH*ed*miesikler nkvikkvvksfsvilakvqmlvlc

  16. The Luminosity Function of M3

    CERN Document Server

    Rood, R T; Paltrinieri, B; Ferraro, F R; Pecci, F F; Dorman, B; Chieffi, A; Straniero, O; Buonanno, R

    1999-01-01

    We present a high precision, large sample luminosity function (LF) for the Galactic globular cluster M3. With a combination of ground based and Hubble Space Telescope data we cover the entire radial extent of the cluster. The observed LF is well fit by canonical standard stellar models from the red giant branch (RGB) tip to below the main sequence turnoff point. Specifically, neither the RGB LF-bump nor subgiant branch LF indicate any breakdown in the standard models. On the main sequence we find evidence for a flat initial mass function and for mass segregation due to the dynamical evolution of the cluster.

  17. Formulation for Oral Delivery of Lactoferrin Based on Bovine Serum Albumin and Tannic Acid Multilayer Microcapsules

    Science.gov (United States)

    Kilic, Ece; Novoselova, Marina V.; Lim, Su Hui; Pyataev, Nikolay A.; Pinyaev, Sergey I.; Kulikov, Oleg A.; Sindeeva, Olga A.; Mayorova, Oksana A.; Murney, Regan; Antipina, Maria N.; Haigh, Brendan; Sukhorukov, Gleb B.; Kiryukhin, Maxim V.

    2017-03-01

    Lactoferrin (Lf) has considerable potential as a functional ingredient in food, cosmetic and pharmaceutical applications. However, the bioavailability of Lf is limited as it is susceptible to digestive enzymes in gastrointestinal tract. The shells comprising alternate layers of bovine serum albumin (BSA) and tannic acid (TA) were tested as Lf encapsulation system for oral administration. Lf absorption by freshly prepared porous 3 μm CaCO3 particles followed by Layer-by-Layer assembly of the BSA-TA shells and dissolution of the CaCO3 cores was suggested as the most efficient and harmless Lf loading method. The microcapsules showed high stability in gastric conditions and effectively protected encapsulated proteins from digestion. Protective efficiency was found to be 76 ± 6% and 85 ± 2%, for (BSA-TA)4 and (BSA-TA)8 shells, respectively. The transit of Lf along the gastrointestinal tract (GIT) of mice was followed in vivo and ex vivo using NIR luminescence. We have demonstrated that microcapsules released Lf in small intestine allowing 6.5 times higher concentration than in control group dosed with the same amount of free Lf. Significant amounts of Lf released from microcapsules were then absorbed into bloodstream and accumulated in liver. Suggested encapsulation system has a great potential for functional foods providing lactoferrin.

  18. Wuchereria bancrofti infection in Haitian immigrants and the risk of re-emergence of lymphatic filariasis in the Brazilian Amazon

    Directory of Open Access Journals (Sweden)

    Edson Fidelis da Silva Junior

    Full Text Available Abstract INTRODUCTION: Lymphatic filariasis (LF is a public health problem in Haiti. Thus, the emigration of Haitians to Brazil is worrisome because of the risk for LF re-emergence. METHODS: Blood samples of Haitian immigrants, aged ≥18 years, who emigrated to Manaus (Brazilian Amazon, were examined using thick blood smears, membrane blood filtration, and immunochromatography. RESULTS: Of the 244 immigrants evaluated, 1 (0.4% tested positive for W. bancrofti; 11.5% reported as having received LF treatment in Haiti. CONCLUSIONS: The re-emergence of LF in Manaus is unlikely, due to its low prevalence and low density of microfilaremia among the assessed Haitian immigrants.

  19. Recombinant Functional Human Lactoferrin Expressed in Baculovirus System

    Institute of Scientific and Technical Information of China (English)

    Tao LIU; Yao-Zhou ZHANG; Xiang-Fu WU

    2006-01-01

    Human lactoferrin (hLf) is a multifunctional iron-binding glycoprotein. In this study, we amplified hLfcDNA by reverse transcription-polymerase chain reaction from normal human mammary gland.The nucleotide sequence of the hLf was identical to the known hLf. We constructed a recombinant virus,vBm-hLf, harboring the hLfgene and exploited the BmN cells as host to produce recombinant human lactoferrin(rhLf). It was found that a recombinant protein with a molecular mass of approximately 78 kDa was expressed.Approximately 13.5 μg rhLf was purified from 1-2× 105 BmN cells infected by vBm-hLf and the rhLf proved to be biologically active. This method established in our study will pave the way for efficient production of rhLf for further application of this protein in the future.

  20. Clinical characteristics of patients with labyrinthine fistulae caused by middle ear cholesteatoma

    Institute of Scientific and Technical Information of China (English)

    YANG Juan-mei; CHI Fang-lu; HAN Zhao; HUANG Yi-bo; LI Yi-ke

    2013-01-01

    Background Labyrinthine fistula (LF) is a very common clinical complication mainly caused by middle ear cholesteatoma.Whether the presence of different degree LF caused by middle ear cholesteatoma aggravates neurosensory hearing loss (NSHL) and what is the degree of the hearing loss caused by LF were still under controversial.This study aimed to investigate whether the LF degree is correlative with the age distribution,disease duration and hearing loss degree for cholesteatomatous patients.Methods The files of 143 patients with middle ear cholesteatoma were selected and reviewed in a retrospective study.Seventy-eight patients with LF were divided into three types according to the degree of destruction of labyrinth.Sixty-five patients without LF were randomly chosen for control.Then,we compared the clinical characteristics of patients with or without labyrinthine fistulae caused by middle ear cholesteatoma.Results According to the study,cholesteatomatous patients with LF were older and suffered longer disease duration than those without LF.Hearing loss is severe with high frequencies both in patients with and without LF.Moreover,inner ear impairment is correlative with the degree of destruction in labyrinth,and more severe destruction in labyrinth follow the more severe symptoms correlative with inner ear impairment.Conclusion Surgical intervention should be performed as eady as possible for these cholesteatomatous patients.

  1. Untersuchungen an Eisen- und Lanthanoidhaltigen Koordinationsclustern mit Triazolat- sowie Semiquinonato-Liganden

    OpenAIRE

    Kriese, Thomas

    2016-01-01

    Magnetische Untersuchungen an Lanthanoiddimeren mit Semiquinonato-Liganden. Gemischtvalente Eisen-Koordinationsclustern. Lanthanoid-Koordinationscluster mit zwölf Lanthanoiden aber mit verschiedenen Clusterladungen.

  2. LIBERACIÓN DE PÉPTIDOS BIOACTIVOS POR BACTERIAS LÁCTICAS EN LECHES FERMENTADAS COMERCIALES

    OpenAIRE

    L.G. González-Olivares; J. Jiménez-Guzmán; A. Cruz-Guerrero; G. Rodríguez-Serrano; L. Gómez-Ruiz; M. García-Garibay

    2011-01-01

    El objetivo de este estudio fue determinar la concentración y el perfil de pesos moleculares de los péptidos bioactivos encriptados en las proteínas de la leche y liberados por la actividad proteolítica de bacterias lácticas en leches fermentadas comerciales, durante su almacenamiento refrigerado. La concentración de péptidos varió de 0.05 mg/ml en LF-3 (Lactobacillus casei) y LF-4 (Lactobacillus acidophilus), a 0.227 mg/ml en LF-5 (Lactobacillus casei y S. thermophilus) y LF-1 (Lactobacillus...

  3. [Development and comparative evaluation of up-converting phosphor technology based lateral flow assay for rapid detection of Yersinia pestis, Bacillus anthracis spore and Brucella spp].

    Science.gov (United States)

    Li, Chunfeng; Zhang, Pingping; Wang, Xiaoying; Liu, Xiao; Zhao, Yong; Sun, Chongyun; Wang, Chengbin; Yang, Ruifu; Zhou, Lei

    2015-01-01

    To develop an up-converting phosphor technology based lateral flow (UPT-LF) assay for rapid and quantitative detection of Yersinia pestis, Bacillus anthracis spore and Brucella spp.and make the comparison with BioThreat Alert (BTA) test strips (Tetracore Inc., USA). Using up-converting phosphor nano-particles (UCP-NPs) as the bio-marker, three double-antibody-sandwich model based UPT-LF strips including Plague-UPT-LF, Anthrax-UPT-LF, Brucella-UPT-LF were prepared and its sensitivity, accuracy, linearity and specificity were determined by detecting 10(10), 10(9), 10(8), 10(7), 10(6), 10(5) and 0 CFU/ml series of concentrations of Y.pestis, B.anthracis, Brucella standards and other 27 kinds of 10(9) CFU/ml series of contrations of bacteria strains.Furthermore, the speed, sensitivity and accuracy of bacteria standards and simulated sample detection were compared between UPT-LF and BTA system. The detection limit of Plague-UPT-LF, Anthrax-UPT-LF and Brucella-LF was 10(5) CFU/ml. The CV of series of bacteria concentrations was ≤ 15%, and the r between lg (T/C-cut-off) and lg (concentration) was 0.996,0.998 and 0.999 (F values were 1 647.57, 743.51 and 1 822.17. All the P values were Brucella-LF were excellent, while that of Anthrax-UPT-LF was a little bit regretful because of non-specific reaction with two isolates of B. subtilis and one B.cereus. On-site evaluation showed the detection time of UPT-LF for all Y.pestis, B.anthracis spore and Brucella spp.was 33, 36 and 37 min, while BTA was 115, 115 and 111 min, which revealed the higher detection speed and sensitivity of UPT-LF comparing with BTA. The negative rate of two methods for blank standard was both 5/5, the sensitivity of UPT-LF for Y.pestis,B.anthracis spore and Brucella spp. was all 10(5) CFU/ml, then BTA was 10(6), 10(6) and 10(5) CFU/ml, respectively. The detection rate of UPT-LF for all three bacteria analog positive samples was 16/16, while BTA for B.anthracis was 7/16 only. The good performance

  4. Dicty_cDB: SSJ679 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available pkkpnyfslnffyyffyyfffffklfhqfy lf*fvvnn*ftdfiqtnnenlltnfgkkkr*k Frame B: flfksykikqc**qksnmksirn*lynknnkar...stfiy*fiy*fiylfiflf--- ---kkkk*kkkkklaqi*qfkllfns*ttnnclklvfllf*lnklxlxxxfkkkilvnfy

  5. Dicty_cDB: AFM182 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available AATTATAAAAAAAAAAXXXXXXXXXX sequence update 2009. 4. 3 Translated Amino Acid sequence kfcsisfniifstiyitltilyk...inskrknknkkk* stnknk*lkipnsfkfeffifififisi*knisi*lf*nylinnykkk--- Frame B: kfcsisfniifstiyitltilyk*elk*nkkks

  6. Compressive myelopathy in fluorosis: MRI

    Energy Technology Data Exchange (ETDEWEB)

    Gupta, R.K. [MR Section, Department of Radiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow-226014 (India); Agarwal, P. [MR Section, Department of Radiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow-226014 (India); Kumar, S. [MR Section, Department of Radiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow-226014 (India); Surana, P.K. [Department of Neurology, SGPGIMS, Lucknow-226014 (India); Lal, J.H. [MR Section, Department of Radiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow-226014 (India); Misra, U.K. [Department of Neurology, SGPGIMS, Lucknow-226014 (India)

    1996-05-01

    We examined four patients with fluorosis, presenting with compressive myelopathy, by MRI, using spin-echo and fast low-angle shot sequences. Cord compression due to ossification of the posterior longitudinal ligament (PLL) and ligamentum flavum (LF) was demonstrated in one and ossification of only the LF in one. Marrow signal was observed in the PLL and LF in all the patients on all pulse sequences. In patients with compressive myelopathy secondary to ossification of PLL and/or LF, fluorosis should be considered as a possible cause, especially in endemic regions. (orig.). With 2 figs., 1 tab.

  7. Dicty_cDB: Contig-U14513-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available KIEEELRKMSKENPSFVTSFCLRSILYAKF*nflct ktycqrshsrshicighaies*nvaigygry**yrfnrccndersn*ilw*nhsilr*lf tw*kdr*nyv...GSDSVFCVTNFWAYFGKELEYGLNIAHAALAAGVKHFV FSGLAPCNKISGGRYNVPHFDLKHKIEEELRKMSKENPSFVTSFCLRSILYAKF*nflct ktycqrshsrshicighaies

  8. Retinoic acid enhances lactoferrin-induced IgA responses by increasing betaglycan expression.

    Science.gov (United States)

    Lee, Jeong-Min; Jang, Young-Saeng; Jin, Bo-Ra; Kim, Sun-Jin; Kim, Hyeon-Jin; Kwon, Bo-Eun; Ko, Hyun-Jeong; Yoon, Sung-Il; Lee, Geun-Shik; Kim, Woan-Sub; Seo, Goo-Young; Kim, Pyeung-Hyeun

    2016-11-01

    Lactoferrin (LF) and retinoic acid (RA) are enriched in colostrum, milk, and mucosal tissues. We recently showed that LF-induced IgA class switching through binding to betaglycan (transforming growth factor-beta receptor III, TβRIII) and activation of canonical TGF-β signaling. We investigated the combined effect of LF and RA on the overall IgA response. An increase in IgA production by LF was further augmented by RA. This combination effect was also evident in Ig germ-line α (GLα) transcription and GLα promoter activity, indicating that LF in cooperation with RA increased IgA isotype switching. We subsequently found that RA enhanced TβRIII expression and that this increase contributed to LF-stimulated IgA production. In addition to the IgA response, LF and RA in combination also enhanced the expression of the gut-homing molecules C-C chemokine receptor 9 (CCR9) and α4β7 on B cells. Finally, peroral administration of LF and RA enhanced the frequency of CCR9(+)IgA(+) plasma cells in the lamina propria. Taken together, these results suggest that LF in cooperation with RA can contribute to the establishment of gut IgA responses.

  9. [In vitro targeting effect of lactoferrin modified PEGylated liposomes for hepatoma cells].

    Science.gov (United States)

    Wei, Min-yan; Zou, Qi; Wu, Chuan-bin; Xu, Yue-hong

    2015-10-01

    A lactoferrin-containing PEGylated liposome system (Lf-PLS) was developed and tested in vitro as a hepatoma-targeting drug delivery system. PEGylated liposomes (PLS) were successfully prepared using the thin film hydration method with peglipid post insertion. Lf was covalently conjugated onto the carboxyl terminal of DSPE-PEG2000-COOH on liposomes. Coumarin-6 was used to trace Lf-PLS with fluorescence. The cellular uptake of this system was carried out in asialoglycoprotein receptor (ASGPR) positive HepG2 cells via confocal microscopy and flow cytometry. The Lf-PLS liposome was observed as spherical or oval vesicles with the particle size around 130 nm, zeta potential about -30 mV and encapsulation efficiency more than 80%. The confocal microscopy images and flow cytometry data demonstrated that Lf-PLS resulted in significantly higher cell association by ASGPR positive HepG2 cells compared to PLS. The association between Lf-PLS and cells were dependent on the concentration, time and temperature, which was inhibited by pre-incubation with excessive free Lf. The results suggest that Lf-PLS has a good targeting effect on HepG2 cells in vitro. The targeting mechanism may be related to the specific binding of Lf and ASGPR on HepG2 cells, which guides Lf-PLS to the cell surface to induce an active endocytosis process. All these results demonstrated that Lf-PLS might be a potential drug delivery system in targeting hepatocellular carcinoma, which deserves more research on its targeting ability, antitumor efficiency, and metabolism in vivo for treatment of hepatomacellular carcinoma.

  10. Soluble Fermentable Dietary Fibre (Pectin Decreases Caloric Intake, Adiposity and Lipidaemia in High-Fat Diet-Induced Obese Rats.

    Directory of Open Access Journals (Sweden)

    Clare L Adam

    Full Text Available Consumption of a high fat diet promotes obesity and poor metabolic health, both of which may be improved by decreasing caloric intake. Satiety-inducing ingredients such as dietary fibre may be beneficial and this study investigates in diet-induced obese (DIO rats the effects of high or low fat diet with or without soluble fermentable fibre (pectin. In two independently replicated experiments, young adult male DIO rats that had been reared on high fat diet (HF; 45% energy from fat were given HF, low fat diet (LF; 10% energy from fat, HF with 10% w/w pectin (HF+P, or LF with 10% w/w pectin (LF+P ad libitum for 4 weeks (n = 8/group/experiment. Food intake, body weight, body composition (by magnetic resonance imaging, plasma hormones, and plasma and liver lipid concentrations were measured. Caloric intake and body weight gain were greatest in HF, lower in LF and HF+P, and lowest in the LF+P group. Body fat mass increased in HF, was maintained in LF, but decreased significantly in LF+P and HF+P groups. Final plasma leptin, insulin, total cholesterol and triglycerides were lower, and plasma satiety hormone PYY concentrations were higher, in LF+P and HF+P than in LF and HF groups, respectively. Total fat and triglyceride concentrations in liver were greatest in HF, lower in LF and HF+P, and lowest in the LF+P group. Therefore, the inclusion of soluble fibre in a high fat (or low fat diet promoted increased satiety and decreased caloric intake, weight gain, adiposity, lipidaemia, leptinaemia and insulinaemia. These data support the potential of fermentable dietary fibre for weight loss and improving metabolic health in obesity.

  11. Lassa Fever in Post-Conflict Sierra Leone

    Science.gov (United States)

    Hartnett, Jessica N.; Levy, Danielle C.; Yenni, Rachael E.; Moses, Lina M.; Fullah, Mohammed; Momoh, Mambo; Fonnie, Mbalu; Fonnie, Richard; Kanneh, Lansana; Koroma, Veronica J.; Kargbo, Kandeh; Ottomassathien, Darin; Muncy, Ivana J.; Jones, Abigail B.; Illick, Megan M.; Kulakosky, Peter C.; Haislip, Allyson M.; Bishop, Christopher M.; Elliot, Deborah H.; Brown, Bethany L.; Zhu, Hu; Hastie, Kathryn M.; Andersen, Kristian G.; Gire, Stephen K.; Tabrizi, Shervin; Tariyal, Ridhi; Stremlau, Mathew; Matschiner, Alex; Sampey, Darryl B.; Spence, Jennifer S.; Cross, Robert W.; Geisbert, Joan B.; Folarin, Onikepe A.; Happi, Christian T.; Pitts, Kelly R.; Geske, F. Jon; Geisbert, Thomas W.; Saphire, Erica Ollmann; Robinson, James E.; Wilson, Russell B.; Sabeti, Pardis C.; Henderson, Lee A.; Khan, S. Humarr; Bausch, Daniel G.; Branco, Luis M.; Garry, Robert F.

    2014-01-01

    Background Lassa fever (LF), an often-fatal hemorrhagic disease caused by Lassa virus (LASV), is a major public health threat in West Africa. When the violent civil conflict in Sierra Leone (1991 to 2002) ended, an international consortium assisted in restoration of the LF program at Kenema Government Hospital (KGH) in an area with the world's highest incidence of the disease. Methodology/Principal Findings Clinical and laboratory records of patients presenting to the KGH Lassa Ward in the post-conflict period were organized electronically. Recombinant antigen-based LF immunoassays were used to assess LASV antigenemia and LASV-specific antibodies in patients who met criteria for suspected LF. KGH has been reestablished as a center for LF treatment and research, with over 500 suspected cases now presenting yearly. Higher case fatality rates (CFRs) in LF patients were observed compared to studies conducted prior to the civil conflict. Different criteria for defining LF stages and differences in sensitivity of assays likely account for these differences. The highest incidence of LF in Sierra Leone was observed during the dry season. LF cases were observed in ten of Sierra Leone's thirteen districts, with numerous cases from outside the traditional endemic zone. Deaths in patients presenting with LASV antigenemia were skewed towards individuals less than 29 years of age. Women self-reporting as pregnant were significantly overrepresented among LASV antigenemic patients. The CFR of ribavirin-treated patients presenting early in acute infection was lower than in untreated subjects. Conclusions/Significance Lassa fever remains a major public health threat in Sierra Leone. Outreach activities should expand because LF may be more widespread in Sierra Leone than previously recognized. Enhanced case finding to ensure rapid diagnosis and treatment is imperative to reduce mortality. Even with ribavirin treatment, there was a high rate of fatalities underscoring the need to

  12. Lassa fever in post-conflict sierra leone.

    Directory of Open Access Journals (Sweden)

    Jeffrey G Shaffer

    2014-03-01

    Full Text Available Lassa fever (LF, an often-fatal hemorrhagic disease caused by Lassa virus (LASV, is a major public health threat in West Africa. When the violent civil conflict in Sierra Leone (1991 to 2002 ended, an international consortium assisted in restoration of the LF program at Kenema Government Hospital (KGH in an area with the world's highest incidence of the disease.Clinical and laboratory records of patients presenting to the KGH Lassa Ward in the post-conflict period were organized electronically. Recombinant antigen-based LF immunoassays were used to assess LASV antigenemia and LASV-specific antibodies in patients who met criteria for suspected LF. KGH has been reestablished as a center for LF treatment and research, with over 500 suspected cases now presenting yearly. Higher case fatality rates (CFRs in LF patients were observed compared to studies conducted prior to the civil conflict. Different criteria for defining LF stages and differences in sensitivity of assays likely account for these differences. The highest incidence of LF in Sierra Leone was observed during the dry season. LF cases were observed in ten of Sierra Leone's thirteen districts, with numerous cases from outside the traditional endemic zone. Deaths in patients presenting with LASV antigenemia were skewed towards individuals less than 29 years of age. Women self-reporting as pregnant were significantly overrepresented among LASV antigenemic patients. The CFR of ribavirin-treated patients presenting early in acute infection was lower than in untreated subjects.Lassa fever remains a major public health threat in Sierra Leone. Outreach activities should expand because LF may be more widespread in Sierra Leone than previously recognized. Enhanced case finding to ensure rapid diagnosis and treatment is imperative to reduce mortality. Even with ribavirin treatment, there was a high rate of fatalities underscoring the need to develop more effective and/or supplemental treatments for

  13. Liver fat has a stronger association with risk factors for type 2 diabetes in African-American compared with Hispanic adolescents.

    Science.gov (United States)

    Alderete, Tanya L; Toledo-Corral, Claudia M; Desai, Preeya; Weigensberg, Marc J; Goran, Michael I

    2013-09-01

    Although overweight and obese African-Americans (AAs) have less visceral adipose tissue (VAT) and liver fat (LF) than Hispanics, they have a similar risk for type 2 diabetes. We examined ethnic differences in the association between VAT and LF with risk factors for type 2 diabetes to help explain this paradox. We conducted a cross-sectional study in an academic pediatric care facility. Subjects were overweight and obese AA (n = 131; 15.5 ± 3.3 years old) and Hispanic adolescents (n = 227; 14.7 ± 3.0 years old). Outcome measures included insulin sensitivity (SI), acute insulin response (AIR), and disposition index (DI) by frequently sampled i.v. glucose tolerance test and minimal modeling. LF, not VAT, was inversely associated with SI, and the effect of high LF compared with low was more pronounced in AAs (P(interaction) < .05). In Hispanics, high LF was associated with a 24% lower SI (P < .01) and a 31% increase in AIR (P < .01) and was not associated with DI (P = .35). In AAs, high LF was associated with a 49% lower SI (P < .001), was not associated with an increase in AIR (P = .25), and was associated with a 42% lower DI (P < .01), indicating failure of compensatory insulin secretion/clearance in response to insulin resistance. Prediabetes changed the relationship between high/low LF and DI in Hispanics (P(interaction) = .002) but not AAs such that prediabetic Hispanics with high LF had a 43% lower DI (P = .03) with no difference in those without prediabetes (P = .06). LF has a stronger effect on SI compared with VAT. Our results suggest that the impact of high LF on poor β-cell compensation is more pronounced in AAs. In Hispanics, the combination of high LF and prediabetes contributes to poor β-cell compensation.

  14. Cleaved inflammatory lactoferrin peptides in parotid saliva of periodontitis patients.

    Science.gov (United States)

    Komine, Ken-Ichi; Kuroishi, Toshinobu; Ozawa, Akiko; Komine, Yumiko; Minami, Takumi; Shimauchi, Hidetoshi; Sugawara, Shunji

    2007-03-01

    Lactoferrin (Lf) is a member of the transferrin family of iron-binding anti-bacterial proteins, present in most exocrine secretions, such as saliva, and plays an important role in mucosal defense. In this study, we identified small Lf peptides with Con A low-affinity in the parotid saliva of chronic periodontitis patients by Con A two-dimensional immunoelectrophoresis, Con A affinity chromatography and Western blotting using anti-human Lf polyclonal Ab. N-terminal amino acid sequencing of the four Con A low-affinity Lf peptides confirmed them to be fragments of intact Lf. The detection ratio of the proteinase 3 (PR3)-like activity was elevated in the parotid saliva of periodontitis patients and was associated with the severity of clinical symptoms. PR3 protein was also detected in the parotid saliva of periodontitis patients, and PR3, but not human leukocyte elastase and cathepsin G, degraded intact Lf. Con A low-affinity saliva Lf peptides showed no anti-bacterial activity against Escherichia coli, and had a reduced iron-chelating capacity. Con A low-affinity saliva Lf peptides, PR3-treated Lf preparation and two of four synthetic polypeptides induced the production of interleukin IL-6, monocyte chemoattractant protein-1 and IL-8, and the activation of NF-kappaB in human oral epithelial HSC-2 cells. Furthermore, concentrations of the Lf peptides in the parotid saliva of periodontitis patients were increased with a correlation to the severity of clinical symptoms. These results suggest that Lf in the parotid saliva of periodontitis patients was degraded into small peptides by the PR3-like activity with the capability to induce inflammatory mediators.

  15. Soluble Fermentable Dietary Fibre (Pectin) Decreases Caloric Intake, Adiposity and Lipidaemia in High-Fat Diet-Induced Obese Rats

    Science.gov (United States)

    Adam, Clare L.; Thomson, Lynn M.; Williams, Patricia A.; Ross, Alexander W.

    2015-01-01

    Consumption of a high fat diet promotes obesity and poor metabolic health, both of which may be improved by decreasing caloric intake. Satiety-inducing ingredients such as dietary fibre may be beneficial and this study investigates in diet-induced obese (DIO) rats the effects of high or low fat diet with or without soluble fermentable fibre (pectin). In two independently replicated experiments, young adult male DIO rats that had been reared on high fat diet (HF; 45% energy from fat) were given HF, low fat diet (LF; 10% energy from fat), HF with 10% w/w pectin (HF+P), or LF with 10% w/w pectin (LF+P) ad libitum for 4 weeks (n = 8/group/experiment). Food intake, body weight, body composition (by magnetic resonance imaging), plasma hormones, and plasma and liver lipid concentrations were measured. Caloric intake and body weight gain were greatest in HF, lower in LF and HF+P, and lowest in the LF+P group. Body fat mass increased in HF, was maintained in LF, but decreased significantly in LF+P and HF+P groups. Final plasma leptin, insulin, total cholesterol and triglycerides were lower, and plasma satiety hormone PYY concentrations were higher, in LF+P and HF+P than in LF and HF groups, respectively. Total fat and triglyceride concentrations in liver were greatest in HF, lower in LF and HF+P, and lowest in the LF+P group. Therefore, the inclusion of soluble fibre in a high fat (or low fat) diet promoted increased satiety and decreased caloric intake, weight gain, adiposity, lipidaemia, leptinaemia and insulinaemia. These data support the potential of fermentable dietary fibre for weight loss and improving metabolic health in obesity. PMID:26447990

  16. Soluble Fermentable Dietary Fibre (Pectin) Decreases Caloric Intake, Adiposity and Lipidaemia in High-Fat Diet-Induced Obese Rats.

    Science.gov (United States)

    Adam, Clare L; Thomson, Lynn M; Williams, Patricia A; Ross, Alexander W

    2015-01-01

    Consumption of a high fat diet promotes obesity and poor metabolic health, both of which may be improved by decreasing caloric intake. Satiety-inducing ingredients such as dietary fibre may be beneficial and this study investigates in diet-induced obese (DIO) rats the effects of high or low fat diet with or without soluble fermentable fibre (pectin). In two independently replicated experiments, young adult male DIO rats that had been reared on high fat diet (HF; 45% energy from fat) were given HF, low fat diet (LF; 10% energy from fat), HF with 10% w/w pectin (HF+P), or LF with 10% w/w pectin (LF+P) ad libitum for 4 weeks (n = 8/group/experiment). Food intake, body weight, body composition (by magnetic resonance imaging), plasma hormones, and plasma and liver lipid concentrations were measured. Caloric intake and body weight gain were greatest in HF, lower in LF and HF+P, and lowest in the LF+P group. Body fat mass increased in HF, was maintained in LF, but decreased significantly in LF+P and HF+P groups. Final plasma leptin, insulin, total cholesterol and triglycerides were lower, and plasma satiety hormone PYY concentrations were higher, in LF+P and HF+P than in LF and HF groups, respectively. Total fat and triglyceride concentrations in liver were greatest in HF, lower in LF and HF+P, and lowest in the LF+P group. Therefore, the inclusion of soluble fibre in a high fat (or low fat) diet promoted increased satiety and decreased caloric intake, weight gain, adiposity, lipidaemia, leptinaemia and insulinaemia. These data support the potential of fermentable dietary fibre for weight loss and improving metabolic health in obesity.

  17. Reload Purified Melittin and Lactoferrin on Perfluorooctyl Bromide Nanoparticles (PFOB-Nps and Examine the Distribution of Particle Size, Zeta Potential and Confirmation of Their Accession on the Nanoparticles Via Tryptophan Fluorescence and Circular Dichroism (CD and its Anti-Cancer Effects on Human Breast Cancer Cell Line MCF7

    Directory of Open Access Journals (Sweden)

    Hadis Rahmani Incheh Keykanlu

    2016-12-01

    Full Text Available Acording to the prevalence of cancer in today's societies, it is too important to find new drugs for that treatment. Cationic antimicrobial peptides that are able to eliminate a wide range of bacteria, fungi, parasites, viruses and unicellular, are natural anti-cancer agents. In the present study, the effect of cationic peptides of bee venom Melittin (ML and Lactoferrin (LF of camel milk which was loaded on the Perfluorooctyl Bromide (PFOB nanoparticles, were examined on MCF7 cells. Nanoparticles using oil in water emulsion was prepared by ultra-thoracic and ultrasonic. Then ML and LF were separately added to the nanoparticles then incorporation of them were confirmed by tryptophan fluorescence and circular dichroism (CD spectra. The mean diameter of the nanoparticles did not change after addition of ML and LF (~100 nm, but as expected, the zeta potential of the nanoparticles with increasing of ML and LF concentrations to the preformed nanoparticles increased from -18.43(without ML and LF to +21.61(LF 110.66µg/ml and +20.93(ML 0.1µg/ml and +23.75(LF 110.66µg/ml and ML 0.1µg/ml. By using of scanning electron microscopy (SEM, the morphology and structure of nanoparticles was investigated, homogeneity and uniformity during processing and loading of ML and LF was observed. Effectiveness of nanoparticles loaded on cancer cells was performed with MTT test. The results showed that ML and LF had a positive effect on cancer cell death. Percent of cell death was higher when ML and LF was loaded on nanoparticles. With increase of ML and LF concentration loading on nanoparticles, increases cancer cell death. Minitab software was used to analyze some data.

  18. Bovine lactoferrin counteracts Toll-like receptor mediated activation signals in antigen presenting cells.

    Directory of Open Access Journals (Sweden)

    Patrizia Puddu

    Full Text Available Lactoferrin (LF, a key element in mammalian immune system, plays pivotal roles in host defence against infection and excessive inflammation. Its protective effects range from direct antimicrobial activities against a large panel of microbes, including bacteria, viruses, fungi and parasites, to antinflammatory and anticancer activities. In this study, we show that monocyte-derived dendritic cells (MD-DCs generated in the presence of bovine LF (bLF fail to undergo activation by up-modulating CD83, co-stimulatory and major histocompatibility complex molecules, and cytokine/chemokine secretion. Moreover, these cells are weak activators of T cell proliferation and retain antigen uptake activity. Consistent with an impaired maturation, bLF-MD-DC primed T lymphocytes exhibit a functional unresponsiveness characterized by reduced expression of CD154 and impaired expression of IFN-γ and IL-2. The observed imunosuppressive effects correlate with an increased expression of molecules with negative regulatory functions (i.e. immunoglobulin-like transcript 3 and programmed death ligand 1, indoleamine 2,3-dioxygenase, and suppressor of cytokine signaling-3. Interestingly, bLF-MD-DCs produce IL-6 and exhibit constitutive signal transducer and activator of transcription 3 activation. Conversely, bLF exposure of already differentiated MD-DCs completely fails to induce IL-6, and partially inhibits Toll-like receptor (TLR agonist-induced activation. Cell-specific differences in bLF internalization likely account for the distinct response elicited by bLF in monocytes versus immature DCs, providing a mechanistic base for its multiple effects. These results indicate that bLF exerts a potent anti-inflammatory activity by skewing monocyte differentiation into DCs with impaired capacity to undergo activation and to promote Th1 responses. Overall, these bLF-mediated effects may represent a strategy to block excessive DC activation upon TLR-induced inflammation, adding

  19. Radial U-band Coma galaxy luminosity functions

    NARCIS (Netherlands)

    Beijersbergen, M; Hoekstra, H; van Dokkum, PG; van der Hulst, JM; Hibbard, JE; Rupen, MP; VanGorkom, JH

    2001-01-01

    We have mapped 1.3 degree(2) of the Coma cluster from the core to beyond the NGC4839 group. Here, we present radial U-band Coma galaxy luminosity functions (LF). The central LF can be represented by a Schechter function, but the radial LFs have very different shapes. We speculate that the derived st

  20. Dicty_cDB: SHB434 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available iscoideum chromosome 2 map 4354771-4414991 strain AX4, complete sequence. 50 2e-08 2 BG453581 |BG453581.1 NF092A07LF1F1050 Developing... sequence. 48 0.26 1 BG453480 |BG453480.1 NF092C08LF1F1055 Developing leaf Medicago truncatula cDNA clone NF