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Sample records for human granulosa-lutein gl

  1. Vasoactive intestinal polypeptide and peptide histidine methionine. Presence in human follicular fluid and effects on DNA synthesis and steroid secretion in cultured human granulosa/lutein cells

    DEFF Research Database (Denmark)

    Gräs, S; Ovesen, P; Andersen, A N

    1994-01-01

    Vasoactive intestinal polypeptide (VIP) and peptide histidine methionine (PHM) originate from the same precursor molecule, prepro VIP. In the present study we examined the concentrations of VIP and PHM in human follicular fluid and their effects on cultured human granulosa/lutein cells. Follicular...... fluid and cells were obtained from patients undergoing in-vitro fertilization for tubal infertility. The concentrations of VIP and PHM in pre-ovulatory human follicular fluid were measured radioimmunochemically. Granulosa/lutein cells isolated from follicular fluid were cultured under serum....... We conclude that VIP and PHM are present in human preovulatory follicular fluid and that VIP stimulates DNA synthesis and oestradiol secretion in cultured human granulosa/lutein cells. This indicates that VIP and perhaps PHM participate in the local nervous regulation of human ovarian function....

  2. Vasoactive intestinal polypeptide and peptide histidine methionine. Presence in human follicular fluid and effects on DNA synthesis and steroid secretion in cultured human granulosa/lutein cells

    DEFF Research Database (Denmark)

    Gräs, S; Ovesen, P; Andersen, A N

    1994-01-01

    fluid and cells were obtained from patients undergoing in-vitro fertilization for tubal infertility. The concentrations of VIP and PHM in pre-ovulatory human follicular fluid were measured radioimmunochemically. Granulosa/lutein cells isolated from follicular fluid were cultured under serum....../l, respectively. VIP at a concentration of 10 nmol/l caused a significant increase in [3H]thymidine incorporation, and at 1000 nmol/l a significant increase in oestradiol secretion was observed. VIP had no effect on progesterone secretion. PHM at the concentrations tested did not influence any of the activities...

  3. Expression and regulation of INTELECTIN1 in human granulosa-lutein cells: role in IGF-1-induced steroidogenesis through NAMPT.

    Science.gov (United States)

    Cloix, Lucie; Reverchon, Maxime; Cornuau, Marion; Froment, Pascal; Ramé, Christelle; Costa, Caroline; Froment, Gisèle; Lecomte, Pierre; Chen, Wenyong; Royère, Dominique; Guerif, Fabrice; Dupont, Joëlle

    2014-08-01

    INTELECTIN (ITLN) is an adipokine involved in the regulation of insulin sensitivity and inflammatory and immunity responses. Serum ITLN levels are lower in obese, diabetic, and polycystic ovary syndrome (PCOS) women than in control subjects. ITLN has never been studied in ovarian cells. Here, we identified ITLN1 in human ovarian follicles and investigated the molecular mechanisms involved in the regulation of its expression in response to the insulin sensitizers metformin and rosiglitazone, in human granulosa-lutein cells (hGLCs) and in a human ovarian granulosa-like tumor cell line (KGN). We also studied the effects of human recombinant ITLN1 (hRom1) on steroid production and on the activation of various signaling pathways. Using RT-PCR, immunoblotting, and immunohistochemistry, we found that INTL1 is present in human follicular cells. Using ELISA, we showed that INTL levels are similar in plasma and follicular fluid (FF) in control patients, whereas they are higher in FF than in plasma in PCOS patients. In KGN cells and hGLCs, insulin (10(-8) M), insulin-like growth factor-1 (IGF-1; 10(-8) M), and metformin (10(-2) M or 10(-3) M) increased INTL1 expression (mRNA and protein) after 12 and 24 h of stimulation. For metformin, this effect was mediated by adenosine monophosphate-activated kinase (PRKA). Furthermore, hRom1 increased nicotinamide phosphoribosyltransferase (NAMPT) expression in KGN and hGLCs. We also showed that hRom1 increased IGF-1-induced progesterone and estradiol secretion and this was associated with an increase in the STAR and CYP19A1 protein levels and an increase in IGF-1R signaling. Furthermore, all these data were abolished when NAMPT was knocked down in KGN cells, suggesting that INTL1 improves IGF-1-induced steroidogenesis through induction of NAMPT in hGLCs. © 2014 by the Society for the Study of Reproduction, Inc.

  4. Expression Levels of the Oxidative Stress Response Gene ALDH3A2 in Granulosa-Lutein Cells Are Related to Female Age and Infertility Diagnosis.

    Science.gov (United States)

    González-Fernández, Rebeca; Hernández, Jairo; Martín-Vasallo, Pablo; Puopolo, Maria; Palumbo, Angela; Ávila, Julio

    2016-05-01

    Oxidative stress (OS) plays an important role in all physiological processes. The effect of OS on cellular processes is modulated by the ability of the cell to express genes implicated in the reversal of lipid, protein, and DNA injury. Aldehyde dehydrogenase 3, member A2 (ALDH3A2) is a ubiquitous enzyme involved in lipid detoxification. The objective of this study was to investigate the expression ofALDH3A2in human granulosa-lutein (GL) cells of women undergoing in vitro fertilization (IVF) and its relationship with age, infertility diagnosis, and IVF outcome variables. Relative expression levels ofALDH3A2were determined by quantitative reverse transcription-polymerase chain reaction. To investigate the effect of age onALDH3A2expression, 72 women between 18 and 44 years of age with no ovarian factor (NOF) were analyzed. To evaluate the effect of infertility diagnosis onALDH3A2expression, the following groups were analyzed: 22 oocyte donors (ODs), 24 women >40 years old (yo) with tubal or male factor and no ovarian pathology, 18 poor responders (PRs), 19 cases with endometriosis (EM), and 18 patients with polycystic ovarian syndrome (PCOS). In NOF,ALDH3A2expression correlated positively with age and with the doses of follicle-stimulating hormone and luteinizing hormone administered and negatively with the number of total and mature oocytes. When different groups were analyzed,ALDH3A2expression levels were higher in patients >40 yo and in PR compared to OD. On the contrary, EM and PCOS levels were lower than expected for age. These data suggest that GL cellALDH3A2expression levels correlate with age, cause of infertility, and ovarian response to stimulation. © The Author(s) 2015.

  5. Metformin enhances the action of insulin on porcine granulosa-lutein cells in vitro.

    Science.gov (United States)

    Lee, Myeong Seop; Kim, Sang Hwan; Kim, Dae Seung; Min, Kwan Sik; Yoon, Jong Taek

    2012-12-01

    Metformin is an oral antidiabetic drug extensively used to treat the polycystic ovary syndrome in women. Metformin increases insulin-stimulated glucose uptake and has direct effects on ovarian steroidogenesis in humans. However, the molecular mechanisms of metformin' action on the ovary are not clear. To investigate the effects of this drug on the insulin-signaling pathway in porcine granulosa cells as an alternative model for human research, we examined the mRNA expressions of porcine insulin receptor (INSR), insulin-like growth factor-1 receptor (IGF-1R), insulin receptor substrate-1 (IRS-1), and the protein activity (activation and phosphorylation) of downstream targets including Raf, mitogen-activated protein kinase (MEK)1/2, extracellular signal regulated kinase (ERK), phosphoinositide-dependent 1 kinase (PDK1), mammalian target of rapamycin (TOR), p70, and nuclear factor-κB (NF-κB) in a primary culture system consisting of porcine granulosa-lutein cells (pGLs) incubated with 10(-5)M metformin and/or 100ng/ml insulin for 24h in a serum-free medium. We also investigated the luciferase activity of transcription factors activator protein-1 (AP-1) and NF-κB. Metformin with insulin significantly increased mRNA expressions of INSR, IGF-1R, and IRS-1, while metformin alone had no significant effect. And metformin with insulin had the significant effect on the protein activity (activation and phosphorylation) of downstream targets of INSR signaling pathway. Metformin with insulin significantly elicited an induction of luciferase activity in the transfection of AP-1 and NF-κBreporter, while metformin alone did not. In conclusion, we examined the activity of metformin and insulin on pGLS in vitro and metformin enhanced the action of insulin on the intracellular signaling pathways. These results suggest that metformin could change the function of ovarian granulosa cells. Copyright © 2012 Elsevier B.V. All rights reserved.

  6. Expression of miR-15a, miR-145, and miR-182 in granulosa-lutein cells, follicular fluid, and serum of women with polycystic ovary syndrome (PCOS).

    Science.gov (United States)

    Naji, Mohammad; Nekoonam, Saeid; Aleyasin, Ashraf; Arefian, Ehsan; Mahdian, Reza; Azizi, Elham; Shabani Nashtaei, Maryam; Amidi, Fardin

    2018-01-01

    Polycystic ovary syndrome (PCOS) is one of the most common endocrinopathies that affects women in reproductive age. MicroRNAs (miRNAs) play crucial roles in normal function of female reproductive system and folliculogenesis. Deregulated expression of miRNAs in PCOS condition may be significantly implicated in the pathogenesis of PCOS. We determined relative expression of miR-15a, miR-145, and miR-182 in granulosa-lutein cells (GLCs), follicular fluid (FF), and serum of PCOS patients. Human subjects were divided into PCOS (n = 20) and control (n = 21) groups. GLCs, FF, and serum were isolated and stored. RNA isolation was performed and cDNA was reversely transcribed using specific stem-loop RT primers. Relative expression of miRNAs was calculated after normalization against U6 expression. Correlation of miRNAs' expression level with basic clinical features and predictive value of miRNAs in FF and serum were appraised. Relative expression of miR-145 and miR-182 in GLCs was significantly decreased in PCOS, but miR-182 in FF of PCOS patients revealed up-regulated levels. Significant correlations between level of miRNAs in FF and serum and hormonal profile of subjects were observed. MiR-182 in FF showed a significant predictive value with AUC of 0.73, 76.4% sensitivity, and 70.5% specificity which was improved after combination of miR-182 and miR-145. A significant dysregulation of miR-145 and miR-182 in GLCs of PCOS may indicate their involvement in pathogenesis of PCOS. Differential up-regulation of miR-182 in FF of PCOS patients with its promising predictive values for discrimination of PCOS reinforced the importance of studying miRNAs' profile in FF.

  7. OpenGL Insights

    CERN Document Server

    Cozzi, Patrick

    2012-01-01

    Get Real-World Insight from Experienced Professionals in the OpenGL Community With OpenGL, OpenGL ES, and WebGL, real-time rendering is becoming available everywhere, from AAA games to mobile phones to web pages. Assembling contributions from experienced developers, vendors, researchers, and educators, OpenGL Insights presents real-world techniques for intermediate and advanced OpenGL, OpenGL ES, and WebGL developers. Go Beyond the Basics The book thoroughly covers a range of topics, including OpenGL 4.2 and recent extensions. It explains how to optimize for mobile devices, explores the design

  8. OpenGL development cookbook

    CERN Document Server

    Movania, Muhammad Mobeen

    2013-01-01

    The book is written in a Cookbook format with practical recipes aimed at helping you exploit OpenGL to its full potential.This book is targeted towards intermediate OpenGL programmers. However, those who are new to OpenGL and know an alternate API like DirectX might also find these recipes useful to create OpenGL animations.

  9. Coordinated Regulation Among Progesterone, Prostaglandins, and EGF-Like Factors in Human Ovulatory Follicles.

    Science.gov (United States)

    Choi, Yohan; Wilson, Kalin; Hannon, Patrick R; Rosewell, Katherine L; Brännström, Mats; Akin, James W; Curry, Thomas E; Jo, Misung

    2017-06-01

    In animal models, the luteinizing hormone surge increases progesterone (P4) and progesterone receptor (PGR), prostaglandins (PTGs), and epidermal growth factor (EGF)-like factors that play essential roles in ovulation. However, little is known about the expression, regulation, and function of these key ovulatory mediators in humans. To determine when and how these key ovulatory mediators are induced after the luteinizing hormone surge in human ovaries. Timed periovulatory follicles were obtained from cycling women. Granulosa/lutein cells were collected from in vitro fertilization patients. The in vivo and in vitro expression of PGR, PTG synthases and transporters, and EGF-like factors were examined at the level of messenger RNA and protein. PGR binding to specific genes was assessed. P4 and PTGs in conditioned media were measured. PGR, PTGS2, and AREG expressions dramatically increased in ovulatory follicles at 12 to 18 hours after human chorionic gonadotropin (hCG). In human granulosa/lutein cell cultures, hCG increased P4 and PTG production and the expression of PGR, specific PTG synthases and transporters, and EGF-like factors, mimicking in vivo expression patterns. Inhibitors for P4/PGR and EGF-signaling pathways reduced hCG-induced increases in PTG production and the expression of EGF-like factors. PGR bound to the PTGS2, PTGES, and SLCO2A1 genes. This report demonstrated the time-dependent induction of PGR, AREG, and PTGS2 in human periovulatory follicles. In vitro studies indicated that collaborative actions of P4/PGR and EGF signaling are required for hCG-induced increases in PTG production and potentiation of EGF signaling in human periovulatory granulosa cells.

  10. Oxalis deppei : Glücksklee (Oxalidaceae)

    OpenAIRE

    Buch, Corinne; Steinecke, Hilke

    2014-01-01

    Als Glück bringendes Mitbringsel erfreut sich der Glücksklee (Oxalis deppei = O. tetraphylla) vor allem zum Jahreswechsel großer Beliebtheit. Neben weiteren, dem (Aber-)Glauben nach Glück bringenden Symbolen wie Schornsteinfeger, Schwein, Hufeisen und Fliegenpilz sind viergeteilte Kleeblätter ein häufiges Motiv auf Silvesterartikeln. Botanisch gehört er allerdings nicht zur Gattung "Klee" (Trifolium, Fabaceae, Schmetterlingsblütler), sondern zur Gattung Sauerklee (Oxalis, Oxalidaceae, Sauerkl...

  11. Neoplasias das glândulas salivares

    OpenAIRE

    Achando, Rita Isabel Sanches Ambrósio

    2010-01-01

    Trabalho final do 5º ano com vista à atribuição do grau de mestre (área científica de patologia) no âmbito do ciclo de estudos de Mestrado Integrado em Medicina Dentária apresentado à Faculdade de Medicina da Universidade de Coimbra. cavidade oral recebe a secreção da saliva de numerosas glândulas que podem classificar-se, quanto ao seu tamanho, em duas categorias: glândulas salivares principais ou major (parótida, submandibular e sublingual) e glândulas salivares acessórias ou minor (labi...

  12. OpenGL 4 shading language cookbook

    CERN Document Server

    Wolff, David

    2013-01-01

    OpenGL Shading Language 4 Cookbook is a hands-on guide that gets straight to the point - actually creating graphics, instead of just theoretical learning. Each recipe is specifically tailored to satisfy your appetite for producing real-time 3-D graphics using the latest GLSL specification.This book is for OpenGL programmers looking to use the modern features of GLSL 4 to create real-time, three-dimensional graphics. Familiarity with OpenGL programming, along with the typical 3D coordinate systems, projections, and transformations is assumed. It can also be useful for experienced GLSL programme

  13. Greased Lightning (GL-10) Flight Testing Campaign

    Science.gov (United States)

    Fredericks, William J.; McSwain, Robert G.; Beaton, Brian F.; Klassman, David W.; Theodore, Colin R.

    2017-01-01

    Greased Lightning (GL-10) is an aircraft configuration that combines the characteristics of a cruise efficient airplane with the ability to perform vertical takeoff and landing (VTOL). This aircraft has been designed, fabricated and flight tested at the small unmanned aerial system (UAS) scale. This technical memorandum will document the procedures and findings of the flight test experiments. The GL-10 design utilized two key technologies to enable this unique aircraft design; namely, distributed electric propulsion (DEP) and inexpensive closed loop controllers. These technologies enabled the flight of this inherently unstable aircraft. Overall it has been determined thru flight test that a design that leverages these new technologies can yield a useful VTOL cruise efficient aircraft.

  14. Warum macht Fliegen glücklich?

    Directory of Open Access Journals (Sweden)

    Gertrud Pfister

    2005-07-01

    Full Text Available Berufliche Orientierungen von Pilotinnen weckt große Erwartungen, die leider nur teilweise eingelöst werden. Es ermöglicht Einsichten in die Berufsbiographien von Pilotinnen und in das „Gendering“ des Berufsfelds, gibt aber auf zentrale Fragen, u.a. nach den Barrieren, die Frauen den Weg an die Spitze versperren, keine befriedigenden Antworten. So bleibt auch offen, warum Fliegen glücklich macht.

  15. O Contrato De Gláucon

    Directory of Open Access Journals (Sweden)

    Luiz Maurício Bentim da Rocha Menezes

    Full Text Available Resumo: O Livro II da República de Platão se inicia com um desafio de Gláucon para Sócrates, onde este deve provar que o homem justo é, de toda maneira, melhor do que o injusto. Para isso, pedirá que Sócrates defenda a justiça por si mesma e censure a injustiça. O discurso de Gláucon pode ser dividido em três partes, sendo a primeira dedicada à origem e à natureza da justiça; a segunda irá indicar a justiça como algo necessário, mas não como um bem; e a terceira, na qual ele irá tentar provar que a vida do injusto é melhor do que a do justo. Neste trabalho, iremos nos centrar em seu primeiro argumento e de que maneira Gláucon defende a justiça através de um contrato.

  16. WebGL for Rosetta Science Planning

    Science.gov (United States)

    Schmidt, Albrecht; Völk, Stefan; Grieger, Björn

    2013-04-01

    Rosetta is a mission of the European Space Agency (ESA) to rendez-vous with comet Churyumov-Gerasimenko in 2014. The trajectory and operations of the mission are particularly complex, have many free parameters and are novel to the community. To support science planning, communicate operational ideas and disseminate operational scenarios to the scientific community, the science ground segment makes use of Web-based visualisation technologies. Using the recent standard WebGL, static pages of time-dependent three-dimensional views of the spacecraft and the field-of-views of the instruments are generated, directly from the operational files. These can then be viewed in modern Web browsers for understanding or verification, be analysed and correlated with other studies. Variable timesteps make it possible to provide both overviews and detailed animated scenes. The technical challenges that are particular to Web-based environments include: (1) In traditional OpenGL, is much easier to compute needed data on demand since the visualisation runs natively on a usually quite powerful computer. In WebGL application, since requests for additional data have to be passed through a Web server, they are more complex and also require a more complex infrastructure. (2) The volume of data that can be kept in a browser environment is limited and has to be transferred over often slow network links. Thus, careful design and reduction of data is required. (3) Although browser support for WebGL has improved since the authors started using it, it is often not well supported on mobile and small devices. (4) Web browsers often only support limited end user interactions with a mouse or keyboards. While some of the challenges can be expected to become less important as technological progress continues, others seem to be more inherent to the approach. On the positive side, the authors' experiences include: (1) low threshold in the community to using the visualisations, (2), thus, cooperative use

  17. Incorporating modern OpenGL into computer graphics education.

    Science.gov (United States)

    Reina, Guido; Muller, Thomas; Ertl, Thomas

    2014-01-01

    University of Stuttgart educators have updated three computer science courses to incorporate forward-compatible OpenGL. To help students, they developed an educational framework that abstracts some of modern OpenGL's difficult aspects.

  18. Antimicrobial effects of GL13K peptide coatings on S. mutans and L. casei

    Science.gov (United States)

    Schnitt, Rebecca Ann

    Background: Enamel breakdown around orthodontic brackets, so-called "white spot lesions", is the most common complication of orthodontic treatment. White spot lesions are caused by bacteria such as Streptococci and Lactobacilli, whose acidic byproducts cause demineralization of enamel crystals. Aims: The aim of this project was to develop an antimicrobial peptide coating for titanium alloy that is capable of killing acidogenic bacteria, specifically Streptococcus mutans and Lactobacillus casei. The long-term goal is to create an antimicrobial-coated orthodontic bracket with the ability to reduce or prevent the formation of white spot lesions in orthodontic patients thereby improving clinical outcomes. Methods: First, an alkaline etching method with NaOH was established to allow effective coating of titanium discs with GL13K, an antimicrobial peptide derived from human saliva. Coatings were verified by contact angle measures, and treated discs were characterized using scanning electron microscopy. Secondly, GL13K coatings were tested against hydrolytic, proteolytic and mechanical challenges to ensure robust coatings. Third, a series of qualitative and quantitative microbiology experiments were performed to determine the effects of GL13K--L and GL13K--D on S. mutans and L. casei, both in solution and coated on titanium. Results: GL13K-coated discs were stable after two weeks of challenges. GL13K--D was effective at killing S. mutans in vitro at low doses. GL13K--D also demonstrated a bactericidal effect on L. casei, however, in contrast to S. mutans, the effect on L. casei was not statistically significant. Conclusion: GL13K--D is a promising candidate for antimicrobial therapy with possible applications for prevention of white spot lesions in orthodontics.

  19. Chemokine Ligand 20: A Signal for Leukocyte Recruitment During Human Ovulation?

    Science.gov (United States)

    Al-Alem, Linah; Puttabyatappa, Muraly; Rosewell, Kathy; Brännström, Mats; Akin, James; Boldt, Jeffrey; Muse, Ken; Curry, Thomas E

    2015-09-01

    Ovulation is one of the cornerstones of female fertility. Disruption of the ovulatory process results in infertility, which affects approximately 10% of couples. Using a unique model in which the dominant follicle is collected across the periovulatory period in women, we have identified a leukocyte chemoattractant, chemokine ligand 20 (CCL20), in the human ovary. CCL20 mRNA is massively induced after an in vivo human chorionic gonadotropin (hCG) stimulus in granulosa (>10 000-fold) and theca (>4000-fold) cells collected during the early ovulatory (12-18 h) and late ovulatory (18-34 h) periods after hCG administration. Because the LH surge sets in motion an inflammatory reaction characterized by an influx of leukocytes and CCL20 is known to recruit leukocytes in other systems, the composition of ovarian leukocytes (CD45+) containing the CCL20 receptor CCR6 was determined immediately prior to ovulation. CD45+/CCR6+ cells were primarily natural killer cells (41%) along with B cells (12%), T cells (11%), neutrophils (10%), and monocytes (9%). Importantly, exogenous CCL20 stimulated ovarian leukocyte migration 59% within 90 minutes. Due to the difficulties in obtaining human follicles, an in vitro model was developed using granulosa-lutein cells to explore CCL20 regulation. CCL20 expression increased 40-fold within 6 hours after hCG, was regulated partially by the epithelial growth factor pathway, and was positively correlated with progesterone production. These results demonstrate that hCG dramatically increases CCL20 expression in the human ovary, that ovarian leukocytes contain the CCL20 receptor, and that CCL20 stimulates leukocyte migration. Our findings raise the prospect that CCL20 may aid in the final ovulatory events and contribute to fertility in women.

  20. A systems approach reveals regulatory circuitry for Arabidopsis trichome initiation by the GL3 and GL1 selectors.

    Directory of Open Access Journals (Sweden)

    Kengo Morohashi

    2009-02-01

    Full Text Available Position-dependent cell fate determination and pattern formation are unique aspects of the development of plant structures. The establishment of single-celled leaf hairs (trichomes from pluripotent epidermal (protodermal cells in Arabidopsis provides a powerful system to determine the gene regulatory networks involved in cell fate determination. To obtain a holistic view of the regulatory events associated with the differentiation of Arabidopsis epidermal cells into trichomes, we combined expression and genome-wide location analyses (ChIP-chip on the trichome developmental selectors GLABRA3 (GL3 and GLABRA1 (GL1, encoding basic helix-loop-helix (bHLH and MYB transcription factors, respectively. Meta-analysis was used to integrate genome-wide expression results contrasting wild type and gl3 or gl1 mutants with changes in gene expression over time using inducible versions of GL3 and GL1. This resulted in the identification of a minimal set of genes associated with the differentiation of epidermal cells into trichomes. ChIP-chip experiments, complemented by the targeted examination of factors known to participate in trichome initiation or patterning, identified about 20 novel GL3/GL1 direct targets. In addition to genes involved in the control of gene expression, such as the transcription factors SCL8 and MYC1, we identified SIM (SIAMESE, encoding a cyclin-dependent kinase inhibitor, and RBR1 (RETINOBLASTOMA RELATED1, corresponding to a negative regulator of the cell cycle transcription factor E2F, as GL3/GL1 immediate targets, directly implicating these trichome regulators in the control of the endocycle. The expression of many of the identified GL3/GL1 direct targets was specific to very early stages of trichome initiation, suggesting that they participate in some of the earliest known processes associated with protodermal cell differentiation. By combining this knowledge with the analysis of genes associated with trichome formation, our results

  1. Loss of Sh3gl2/Endophilin A1 Is a Common Event in Urothelial Carcinoma that Promotes Malignant Behavior

    Directory of Open Access Journals (Sweden)

    Shyama Majumdar

    2013-07-01

    Full Text Available Urothelial carcinoma (UC causes substantial morbidity and mortality worldwide. However, the molecular mechanisms underlying urothelial cancer development and tumor progression are still largely unknown. Using informatics analysis, we identified Sh3gl2 (endophilin A1 as a bladder urothelium-enriched transcript. The gene encoding Sh3gl2 is located on chromosome 9p, a region frequently altered in UC. Sh3gl2 is known to regulate endocytosis of receptor tyrosine kinases implicated in oncogenesis, such as the epidermal growth factor receptor (EGFR and c-Met. However, its role in UC pathogenesis is unknown. Informatics analysis of expression profiles as well as immunohistochemical staining of tissue microarrays revealed Sh3gl2 expression to be decreased in UC specimens compared to nontumor tissues. Loss of Sh3gl2 was associated with increasing tumor grade and with muscle invasion, which is a reliable predictor of metastatic disease and cancer-derived mortality. Sh3gl2 expression was undetectable in 19 of 20 human UC cell lines but preserved in the low-grade cell line RT4. Stable silencing of Sh3gl2 in RT4 cells by RNA interference 1 enhanced proliferation and colony formation in vitro, 2 inhibited EGF-induced EGFR internalization and increased EGFR activation, 3 stimulated phosphorylation of Src family kinases and STAT3, and 4 promoted growth of RT4 xenografts in subrenal capsule tissue recombination experiments. Conversely, forced re-expression of Sh3gl2 in T24 cells and silenced RT4 clones attenuated oncogenic behaviors, including growth and migration. Together, these findings identify loss of Sh3gl2 as a frequent event in UC development that promotes disease progression.

  2. Loss of Sh3gl2/endophilin A1 is a common event in urothelial carcinoma that promotes malignant behavior.

    Science.gov (United States)

    Majumdar, Shyama; Gong, Edward M; Di Vizio, Dolores; Dreyfuss, Jonathan; Degraff, David J; Hager, Martin H; Park, Peter J; Bellmunt, Joaquim; Matusik, Robert J; Rosenberg, Jonathan E; Adam, Rosalyn M

    2013-07-01

    Urothelial carcinoma (UC) causes substantial morbidity and mortality worldwide. However, the molecular mechanisms underlying urothelial cancer development and tumor progression are still largely unknown. Using informatics analysis, we identified Sh3gl2 (endophilin A1) as a bladder urothelium-enriched transcript. The gene encoding Sh3gl2 is located on chromosome 9p, a region frequently altered in UC. Sh3gl2 is known to regulate endocytosis of receptor tyrosine kinases implicated in oncogenesis, such as the epidermal growth factor receptor (EGFR) and c-Met. However, its role in UC pathogenesis is unknown. Informatics analysis of expression profiles as well as immunohistochemical staining of tissue microarrays revealed Sh3gl2 expression to be decreased in UC specimens compared to nontumor tissues. Loss of Sh3gl2 was associated with increasing tumor grade and with muscle invasion, which is a reliable predictor of metastatic disease and cancer-derived mortality. Sh3gl2 expression was undetectable in 19 of 20 human UC cell lines but preserved in the low-grade cell line RT4. Stable silencing of Sh3gl2 in RT4 cells by RNA interference 1) enhanced proliferation and colony formation in vitro, 2) inhibited EGF-induced EGFR internalization and increased EGFR activation, 3) stimulated phosphorylation of Src family kinases and STAT3, and 4) promoted growth of RT4 xenografts in subrenal capsule tissue recombination experiments. Conversely, forced re-expression of Sh3gl2 in T24 cells and silenced RT4 clones attenuated oncogenic behaviors, including growth and migration. Together, these findings identify loss of Sh3gl2 as a frequent event in UC development that promotes disease progression.

  3. The GL(1 vertical stroke 1)-symplectic fermion correspondence

    Energy Technology Data Exchange (ETDEWEB)

    Creutzig, Thomas; Roenne, Peter B.

    2008-12-15

    In this note we prove a correspondence between the Wess-Zumino-Novikov-Witten model of the Lie supergroup GL(1 vertical stroke 1) and a free model consisting of two scalars and a pair of symplectic fermions. This model was discussed earlier by LeClair. Vertex operators for the symplectic fermions include twist fields, and correlation functions of GL(1 vertical stroke 1) agree with the known results for the scalars and symplectic fermions. We perform a detailed study of boundary states for symplectic fermions and apply them to branes in GL(1 vertical stroke 1). This allows us to compute new amplitudes of strings stretching between branes of different types and confirming Cardy's condition. (orig.)

  4. Arterite temporal com comprometimento das glândulas salivares

    Directory of Open Access Journals (Sweden)

    Lineu C. Werneck

    1972-09-01

    Full Text Available E relatado um caso de arterite temporal em paciente com crises de cefaléia paroxística associadas a síndrome depressiva e, na última crise, a aumento de volume das glândulas salivares. Biopsia de artéria temporal superficial mostrou aspecto típico de arterite, com células gigantes mononucleadas. A cefalalgia e o aumento de volume das glândulas salivares regrediram mediante a administração de corticoesteróides.

  5. Branes in the GL(1|1) WZNW-model

    NARCIS (Netherlands)

    Creutzig, T.; Quella, T.; Schomerus, V.

    2008-01-01

    We initiate a systematic study of boundary conditions in conformal field theories with target space supersymmetry. The WZNW model on GL(1|1) is used as a prototypical example for which we find the complete set of maximally symmetric branes. This includes a unique brane of maximal super-dimension

  6. Astrocitoma de grado I en glándula pineal

    OpenAIRE

    Iglesias Rozas, José Rafael, 1942-

    1985-01-01

    Tres imágenes de un astrocitoma de grado I situado en la glándula pineal de un paciente de 10 años. Three pictures of a grade I astrocytoma located in the pineal gland of a 10-year-old male patient.

  7. OpenGL ES 3.0 cookbook

    CERN Document Server

    Singh, Parminder

    2015-01-01

    If you are new to OpenGL ES or have some experience in 3D graphics, then this book will be extremely helpful in raising your expertise level from a novice to professional. The book implements more than 90 recipes to solve everyday challenges, helping you transition from a beginner to a professional.

  8. Birgit Steinbügl: Deutsch-englische Kollokationen

    African Journals Online (AJOL)

    R.B. Ruthven

    Birgit Steinbügl. Deutsch–englische Kollokationen: Erfassung in zweispra- chigen Wörterbüchern und Grenzen der korpusbasierten Analyse. ..... Lexikographie und Grammatik: Akten des Essener Kolloquiums zur Grammatik im Wörterbuch 28–. 30.6.1984: 118-129. Lexicographica. Series Maior 3. Tübingen: Max Niemeyer.

  9. The Case for Teaching Computer Graphics with WebGL: A 25-Year Perspective.

    Science.gov (United States)

    Angel, Ed

    2017-01-01

    OpenGL has been the standard API for teaching computer graphics. There are now multiple versions of the standard, including WebGL. which is the JavaScript implementation of OpenGL ES 2.0. The author argues that WebGL is the version best suited for an introductory course in computer graphics.

  10. Employing WebGL to develop interactive stereoscopic 3D content for use in biomedical visualization

    Science.gov (United States)

    Johnston, Semay; Renambot, Luc; Sauter, Daniel

    2013-03-01

    Web Graphics Library (WebGL), the forthcoming web standard for rendering native 3D graphics in a browser, represents an important addition to the biomedical visualization toolset. It is projected to become a mainstream method of delivering 3D online content due to shrinking support for third-party plug-ins. Additionally, it provides a virtual reality (VR) experience to web users accommodated by the growing availability of stereoscopic displays (3D TV, desktop, and mobile). WebGL's value in biomedical visualization has been demonstrated by applications for interactive anatomical models, chemical and molecular visualization, and web-based volume rendering. However, a lack of instructional literature specific to the field prevents many from utilizing this technology. This project defines a WebGL design methodology for a target audience of biomedical artists with a basic understanding of web languages and 3D graphics. The methodology was informed by the development of an interactive web application depicting the anatomy and various pathologies of the human eye. The application supports several modes of stereoscopic displays for a better understanding of 3D anatomical structures.

  11. 3D Web-based HMI with WebGL Rendering Performance

    Directory of Open Access Journals (Sweden)

    Muennoi Atitayaporn

    2016-01-01

    Full Text Available An HMI, or Human-Machine Interface, is a software allowing users to communicate with a machine or automation system. It usually serves as a display section in SCADA (Supervisory Control and Data Acquisition system for device monitoring and control. In this papper, a 3D Web-based HMI with WebGL (Web-based Graphics Library rendering performance is presented. The main purpose of this work is to attempt to reduce the limitations of traditional 3D web HMI using the advantage of WebGL. To evaluate the performance, frame rate and frame time metrics were used. The results showed 3D Web-based HMI can maintain the frame rate 60FPS for #cube=0.5K/0.8K, 30FPS for #cube=1.1K/1.6K when it was run on Internet Explorer and Chrome respectively. Moreover, the study found that 3D Web-based HMI using WebGL contains similar frame time in each frame even though the numbers of cubes are up to 5K. This indicated stuttering incurred less in the proposed 3D Web-based HMI compared to the chosen commercial HMI product.

  12. Oxygenation by a superhydrophobic slip G/L contactor.

    Science.gov (United States)

    Karatay, Elif; Lammertink, Rob G H

    2012-08-21

    The compelling need for an efficient supply of gases into liquids or degassing of fluids within confined microchannels triggered our study on membrane assisted microchemical systems. Porous hydrophobic flat/micro-structured polyvinylidene fluoride (PVDF) membranes were fabricated and integrated in a glass G/L contacting microfluidic device with the aid of optical adhesives. The oxygen transport in microchannels, driven by convection and diffusion, was investigated both experimentally and numerically. The effects of intrinsic membrane morphology on the G/L contacting performance of the resultant membranes were studied. The experimental performance of the flat membranes are shown to obey the simulation results with the assumptions of negligible gas phase and membrane mass transfer limitations. Micro-structured membranes revealed apparent slippage and enhanced mass transport rates, and exceeded the experimental performance of the flat membranes.

  13. The physico-chemical characteristics of the phosphocholine-containing glycoglycerolipid MfGL-II govern the permeability properties of Mycoplasma fermentans.

    Science.gov (United States)

    Ben-Menachem, G; Byström, T; Rechnitzer, H; Rottem, S; Rilfors, L; Lindblom, G

    2001-07-01

    Mycoplasma fermentans seems to be involved in several pathogenic conditions in humans, and is among other things capable of fusing with T-cells and lymphocytes. The choline-containing phosphoglycolipid 6'-O-(3"-phosphocholine-2"-amino-1"-phospho-1",3"-propanediol)-alpha-D-glucopyranosyl-(1'-->3)-1,2-diacylglycerol (MfGL-II) in the membrane of M. fermentans has been suggested to enhance the fusion process, and the characteristics of MfGL-II were therefore investigated. When a cell culture ages the fraction of MfGL-II increases, and the fraction of the other major membrane lipid, phosphatidylglycerol (PtdGro), decreases concomitantly. Swelling experiments showed that the permeability and osmotic fragility are markedly reduced in aged cells. MfGL-II is selectively released into the surrounding medium when aged M. fermentans cells are incubated in buffer containing EDTA. The physico-chemical properties of MfGL-II were studied by NMR spectroscopy and differential scanning calorimetry, and they can explain the biochemical results. The temperature for the transition between gel and lamellar liquid crystalline (Lalpha) phases is 35-45 degrees C higher for MfGL-II than for PtdGro, which most probably gives rise to the reduced permeability in aged cells. At high water contents MfGL-II forms an Lalpha phase and isotropic aggregates which were interpreted to be vesicles with a radius of approximately 450 A. It is proposed that MfGL-II forms vesicles in the surrounding medium when it is released from the cell membrane. Neither EDTA nor Ca2+ ions have a significant influence on the aggregate structures formed by MfGL-II. Our results indicate that MfGL-II has no fusogenic properties. It is more probable that a recently identified lysolipid in the M. fermentans membrane acts as a fusogen.

  14. Millisecond accuracy video display using OpenGL under Linux.

    Science.gov (United States)

    Stewart, Neil

    2006-02-01

    To measure people's reaction times to the nearest millisecond, it is necessary to know exactly when a stimulus is displayed. This article describes how to display stimuli with millisecond accuracy on a normal CRT monitor, using a PC running Linux. A simple C program is presented to illustrate how this may be done within X Windows using the OpenGL rendering system. A test of this system is reported that demonstrates that stimuli may be consistently displayed with millisecond accuracy. An algorithm is presented that allows the exact time of stimulus presentation to be deduced, even if there are relatively large errors in measuring the display time.

  15. Tutorial web para el aprendizaje de WebGL

    OpenAIRE

    Sanz Asenjo, Alejandro

    2016-01-01

    Este proyecto abordará la tecnología WebGL, que permite la presentación de gráficos 3D en páginas web, y la dificultad que existe actualmente en su estudio y enseñanza. Es una tecnología nueva, con pocos ejemplos en la web o en los libros y que tiene una alta curva de aprendizaje. Debido a esto, el objetivo de este proyecto es obtener un sistema web de aprendizaje basado en tutoriales, el cual será utilizado como herramienta docente en la asignatura “Programación de Aplicaciones Grá...

  16. Evaluation of Gløshaugen wind conditions

    OpenAIRE

    Lopez Pareja, Alejandro

    2016-01-01

    It is very important to know the wind conditions when, for example, designing buildings. In this paper, the results focus on the wind conditions needed to decide if it is interesting and right to deploy some wind turbines at the roofs of Gløshaugen s buildings in order to the university becomes energy self-sufficient; or at least more independent by generating part of its own energy. To do that, a ZephIR 300 Lidar takes wind conditions data that is analyzed and present here. Moreover, an AIR-...

  17. Learning game physics with Bullet Physics and OpenGL

    CERN Document Server

    Dickinson, Chris

    2013-01-01

    A comprehensive set of straight-forward, easy-to-follow tutorials in OpenGL and Bullet Physics that will teach you how modern game physics and 3D graphics work.If you're a beginner or intermediate programmer with a basic understanding of 3D mathematics, and you want a stronger foundation in 3D graphics and physics, then this book is perfect for you! You'll even learn some of the fundamental concepts in 3D mathematics and software design that lies beneath them both, discovering some techniques and tricks in graphics and physics that you can use in any game development project.

  18. SM-ROM-GL (Strong Motion Romania Ground Level Database

    Directory of Open Access Journals (Sweden)

    Ioan Sorin BORCIA

    2015-07-01

    Full Text Available The SM-ROM-GL database includes data obtained by the processing of records performed at ground level by the Romanian seismic networks, namely INCERC, NIEP, NCSRR and ISPH-GEOTEC, during recent seismic events with moment magnitude Mw ≥ 5 and epicenters located in Romania. All the available seismic records were re-processed using the same basic software and the same procedures and options (filtering and baseline correction, in order to obtain a consistent dataset. The database stores computed parameters of seismic motions, i.e. peak values: PGA, PGV, PGD, effective peak values: EPA, EPV, EPD, control periods, spectral values of absolute acceleration, relative velocity and relative displacement, as well as of instrumental intensity (as defined bz Sandi and Borcia in 2011. The fields in the database include: coding of seismic events, stations and records, a number of associated fields (seismic event source parameters, geographical coordinates of seismic stations, links to the corresponding ground motion records, charts of the response spectra of absolute acceleration, relative velocity, relative displacement and instrumental intensity, as well as some other representative parameters of seismic motions. The conception of the SM-ROM-GL database allows for an easy maintenance; such that elementary knowledge of Microsoft Access 2000 is sufficient for its operation.

  19. El glóbulo rojo en cifras

    Directory of Open Access Journals (Sweden)

    Mauricio Boyacá

    2004-11-01

    Full Text Available El glóbulo rojo es una célula de 5 a 7 micras de diámetro, se encarga de transportar oxígeno desde los pulmones hacia las células de los tejidos, mediante su adhesión a la hemoglobina, cuyo componente principal es el hierro, igualmente, cataliza la formacion de bicarbonato a partir del agua, y del dióxido de carbono, para que este último retorne a los pulmones disuelto en la sangre y ser eliminado, manteniendo viables las células y tejidos. Estas células sanguineas tienen un pequeño tamaño y ocupan un volumen mínimo medido en femtolitos (FI, ellas trasnportan una cantidad diminuta de hemoglobina medida en picogramos (Pg. La porducción de eritrocitos ocurre en la médula ósea de las especies superiores, en una relación aproximada de 35 milones de células por segundo. Estos viven 100 días en promedio, y mueren en un número similar. Los glóbulos rojos son depositados en el brazo, para reciclar sus componentes, ensamblados luego por la médula ósea como células nuevas. En consecuencia, un animal con un peso de 450 kilogramos, tendrá cerca de 300 billones de células circulando en el sistema vascular.

  20. Loss of Sh3gl2/Endophilin A1 Is a Common Event in Urothelial Carcinoma that Promotes Malignant Behavior12

    Science.gov (United States)

    Majumdar, Shyama; Gong, Edward M; Di Vizio, Dolores; Dreyfuss, Jonathan; DeGraff, David J; Hager, Martin H; Park, Peter J; Bellmunt, Joaquim; Matusik, Robert J; Rosenberg, Jonathan E; Adam, Rosalyn M

    2013-01-01

    Urothelial carcinoma (UC) causes substantial morbidity and mortality worldwide. However, the molecular mechanisms underlying urothelial cancer development and tumor progression are still largely unknown. Using informatics analysis, we identified Sh3gl2 (endophilin A1) as a bladder urothelium-enriched transcript. The gene encoding Sh3gl2 is located on chromosome 9p, a region frequently altered in UC. Sh3gl2 is known to regulate endocytosis of receptor tyrosine kinases implicated in oncogenesis, such as the epidermal growth factor receptor (EGFR) and c-Met. However, its role in UC pathogenesis is unknown. Informatics analysis of expression profiles as well as immunohistochemical staining of tissue microarrays revealed Sh3gl2 expression to be decreased in UC specimens compared to nontumor tissues. Loss of Sh3gl2 was associated with increasing tumor grade and with muscle invasion, which is a reliable predictor of metastatic disease and cancer-derived mortality. Sh3gl2 expression was undetectable in 19 of 20 human UC cell lines but preserved in the low-grade cell line RT4. Stable silencing of Sh3gl2 in RT4 cells by RNA interference 1) enhanced proliferation and colony formation in vitro, 2) inhibited EGF-induced EGFR internalization and increased EGFR activation, 3) stimulated phosphorylation of Src family kinases and STAT3, and 4) promoted growth of RT4 xenografts in subrenal capsule tissue recombination experiments. Conversely, forced re-expression of Sh3gl2 in T24 cells and silenced RT4 clones attenuated oncogenic behaviors, including growth and migration. Together, these findings identify loss of Sh3gl2 as a frequent event in UC development that promotes disease progression. PMID:23814487

  1. Learn OpenGL ES for mobile game and graphics development

    CERN Document Server

    Mehta, Prateek

    2013-01-01

    One of the first OpenGL ES version 3 books for game app developers doing mobile. Covers Android code and may be useful to iOS game developers too. OpenGL ES 3 is a new significant tech update to OpenGL ES graphics API engine that fuels game apps in both iOS and Android.

  2. Building a Laboratory Information Management System Using Windows4GL

    Energy Technology Data Exchange (ETDEWEB)

    Pickens, M.A.; Shaieb, M.R.

    1996-05-01

    The system discussed is currently implemented at LLNL in the Environmental Services program which operates out of the Chemistry & Materials Science (C&MS) directorate. Responsibility is to provide the C&MS Environmental Services (CES) program with an enterprise-wide information system which will aid CES. The specific portion of the information system is the Sample Tracking, Analysis and Reporting System (STARS). Since CES was formed by merging two analytical laboratory organizations in May 1995, a new Laboratory Information Management System (LIMS) had to be developed. The development of a LIMS in Windows4GL was found to be satisfactory. The product STARS was well received by the user community, and it has improved business practices and efficiency in CES. The CES management staff has seen increased personnel productivity since STARS was release. We look forward to upgrading to CA-OpenROAD and taking advantage of its many improved and innovative features to further enhance STARS.

  3. Frog: The fast & realistic OpenGL event displayer

    Science.gov (United States)

    Quertenmont, Loïc

    2010-04-01

    FROG [1] [2] is a generic framework dedicated to visualisation of events in high energy physics experiment. It is suitable to any particular physics experiment or detector design. The code is light (cross-platform OpenGL[3] and Glut [4] libraries. Moreover, Frog does not require installation of heavy third party libraries for the visualisation. This documents describes the features and principles of Frog version 1.106, its working scheme and numerous functionalities such as: 3D and 2D visualisation, graphical user interface, mouse interface, configuration files, production of pictures of various format, integration of personal objects, etc. Finally the application of FROG for physic experiment/environement, such as Gastof, CMS, ILD, Delphes will be presented for illustration.

  4. Frog: The Fast & Realistic OpenGL Event Displayer

    CERN Document Server

    Quertenmont, Loic

    2009-01-01

    FROG [1] is a generic framework dedicated to visualisation of events in high energy experiment. It is suitable for any particular physics experiment or detector design. The code is light ($<3~\\textrm{MB}$) and fast (browsing time $\\sim20$ events per second for a large High Energy Physics experiment) and can run on various operating systems, as its object-oriented structure (C++) relies on the cross-platform OpenGL[2] and GLUT [3] libraries. Moreover, \\textsc{Frog} does not require installation of third party libraries for the visualisation. This documents describes the features and principles of FROG version 1.106, its working scheme and numerous functionalities such as: 3D and 2D visualisation, graphical user interface, mouse interface, configuration files, production of pictures of various format, integration of personal objects, etc. Finally, several examples of its current applications are presented for illustration.

  5. The GL service: Web service to exchange GL string encoded HLA & KIR genotypes with complete and accurate allele and genotype ambiguity.

    Science.gov (United States)

    Milius, Robert P; Heuer, Michael; George, Mike; Pollack, Jane; Hollenbach, Jill A; Mack, Steven J; Maiers, Martin

    2016-03-01

    Genotype list (GL) Strings use a set of hierarchical character delimiters to represent allele and genotype ambiguity in HLA and KIR genotypes in a complete and accurate fashion. A RESTful web service called genotype list service was created to allow users to register a GL string and receive a unique identifier for that string in the form of a URI. By exchanging URIs and dereferencing them through the GL service, users can easily transmit HLA genotypes in a variety of useful formats. The GL service was developed to be secure, scalable, and persistent. An instance of the GL service is configured with a nomenclature and can be run in strict or non-strict modes. Strict mode requires alleles used in the GL string to be present in the allele database using the fully qualified nomenclature. Non-strict mode allows any GL string to be registered as long as it is syntactically correct. The GL service source code is free and open source software, distributed under the GNU Lesser General Public License (LGPL) version 3 or later. Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

  6. Tumores de glándula mamaria en caninos

    Directory of Open Access Journals (Sweden)

    Giovanni Torres

    2005-10-01

    Full Text Available De los animales de compañía, los caninos son los que sufren con mayor frecuencia tumores de glándula mamaria y dentro de ellos las hembras son las que contribuyen con el mayor número de casos. Cuando estos tumores se presentan en machos por lo general tienen características histológicas malignas. Para el diagnóstico histopatológico la clasificación que ha tenido mayor aceptación es la propuesta por Hampe and Misdorp. A través de muchos años de investigación en este campo se han descrito varios factores considerados como predisponentes, entre los cuales están: raza, sexo, edad, dentro de estos la que presenta mayor disparidad de conceptos es la raza ya que, según algunos autores, se puede ver influenciada por factores externos como la localización geográfica y el gusto de los propietarios por ciertas razas como animales de compañía. Otro hecho importante es la descripción de agentes causales: el invalance hormonal (estrógenos, progesterona, mutaciones genéticas, (gen supresor del tumor, protooncogenes y consumo de dietas ricas en grasas. Finalmente, es importante hacer énfasis en el hecho que del diagnóstico adecuado y pautas de tratamiento llevados a cabo por el médico veterinario clínico, depende el pronóstico y las expectativas de vida del paciente canino.

  7. Professional WebGL Programming Developing 3D Graphics for the Web

    CERN Document Server

    Anyuru, Andreas

    2012-01-01

    Everything you need to know about developing hardware-accelerated 3D graphics with WebGL! As the newest technology for creating 3D graphics on the web, in both games, applications, and on regular websites, WebGL gives web developers the capability to produce eye-popping graphics. This book teaches you how to use WebGL to create stunning cross-platform apps. The book features several detailed examples that show you how to develop 3D graphics with WebGL, including explanations of code snippets that help you understand the why behind the how. You will also develop a stronger understanding of W

  8. SeqGL Identifies Context-Dependent Binding Signals in Genome-Wide Regulatory Element Maps.

    Directory of Open Access Journals (Sweden)

    Manu Setty

    2015-05-01

    Full Text Available Genome-wide maps of transcription factor (TF occupancy and regions of open chromatin implicitly contain DNA sequence signals for multiple factors. We present SeqGL, a novel de novo motif discovery algorithm to identify multiple TF sequence signals from ChIP-, DNase-, and ATAC-seq profiles. SeqGL trains a discriminative model using a k-mer feature representation together with group lasso regularization to extract a collection of sequence signals that distinguish peak sequences from flanking regions. Benchmarked on over 100 ChIP-seq experiments, SeqGL outperformed traditional motif discovery tools in discriminative accuracy. Furthermore, SeqGL can be naturally used with multitask learning to identify genomic and cell-type context determinants of TF binding. SeqGL successfully scales to the large multiplicity of sequence signals in DNase- or ATAC-seq maps. In particular, SeqGL was able to identify a number of ChIP-seq validated sequence signals that were not found by traditional motif discovery algorithms. Thus compared to widely used motif discovery algorithms, SeqGL demonstrates both greater discriminative accuracy and higher sensitivity for detecting the DNA sequence signals underlying regulatory element maps. SeqGL is available at http://cbio.mskcc.org/public/Leslie/SeqGL/.

  9. Antimicrobial GL13K peptide coatings killed and ruptured the wall of Streptococcus gordonii and prevented formation and growth of biofilms.

    Directory of Open Access Journals (Sweden)

    Xi Chen

    Full Text Available Infection is one of the most prevalent causes for dental implant failure. We have developed a novel antimicrobial peptide coating on titanium by immobilizing the antimicrobial peptide GL13K. GL13K was developed from the human salivary protein BPIFA2. The peptide exhibited MIC of 8 µg/ml against planktonic Pseudonomas aeruginosa and their biofilms were reduced by three orders of magnitude with 100 µg/ml GL13K. This peptide concentration also killed 100% of Streptococcus gordonii. At 1 mg/ml, GL13K caused less than 10% lysis of human red blood cells, suggesting low toxicity to mammalian cells. Our GL13K coating has also previously showed bactericidal effect and inhibition of biofilm growth against peri-implantitis related pathogens, such as Porphyromonas gingivalis. The GL13K coating was cytocompatible with human fibroblasts and osteoblasts. However, the bioactivity of antimicrobial coatings has been commonly tested under (quasistatic culture conditions that are far from simulating conditions for biofilm formation and growth in the oral cavity. Oral salivary flow over a coating is persistent, applies continuous shear forces, and supplies sustained nutrition to bacteria. This accelerates bacteria metabolism and biofilm growth. In this work, the antimicrobial effect of the coating was tested against Streptococcus gordonii, a primary colonizer that provides attachment for the biofilm accretion by P. gingivalis, using a drip-flow biofilm bioreactor with media flow rates simulating salivary flow. The GL13K peptide coatings killed bacteria and prevented formation and growth of S. gordonii biofilms in the drip-flow bioreactor and under regular mild-agitation conditions. Surprisingly the interaction of the bacteria with the GL13K peptide coatings ruptured the cell wall at their septum or polar areas leaving empty shell-like structures or exposed protoplasts. The cell wall rupture was not detected under regular culture conditions, suggesting that cell

  10. Bone morphogenetic protein 2 regulates cell-cell communication by down-regulating connexin43 expression in luteinized human granulosa cells.

    Science.gov (United States)

    Wu, Yan-Ting; Chang, Hsun-Ming; Huang, He-Feng; Sheng, Jian-Zhong; Leung, Peter C K

    2017-03-01

    Does bone morphogenetic protein 2 (BMP2) regulate connexin43 (Cx43) and modulate cell-cell communication in luteinized human granulosa cells? BMP2 decreases gap junction intercellular communication (GJIC) of luteinized human granulosa cells by down-regulating Cx43 expression through an activin receptor-like kinase (ALK)2/ALK3-mediated Sma- and Mad-related protein (SMAD)-dependent signaling pathway. BMP2 and its putative receptors are highly expressed in the human corpus luteum and are involved in the process of luteolysis. Cx43-coupled gap junctions play a critical role in the development and maintenance of corpus luteum. This is a laboratory study conducted over a 1-year period. At least three independent experiments with three replicates were conducted and the experimental samples were compared with the appropriate vehicle controls for all of the inhibition-approach, concentration-dependent or time-course studies. SVOG cell line (immortalized human granulosa-lutein cells derived from in vitro fertilization patients in an academic research center) was used as the study model. The changes of Cx43 expression and levels of phosphorylated SMAD1/5/8 protein were evaluated after exposure to recombinant human BMP2. Real-time quantitative PCR and Western blot analysis were used to examine the specific mRNA and protein levels, respectively. The BMP/TGF-β type I receptor inhibitors (Dorsomorphin, DMH-1 and SB431542) and target depletion small interfering RNAs (ALK2, ALK3, ALK6 and SMAD4) were used to investigate the underlying molecular mechanisms. A scrape loading and dye transfer assay was used to evaluate the GJIC between the SVOG cells. Treatment with BMP2 down-regulated the expression of Cx43 and decreased the GJIC activity, whereas it increased the phosphorylated SMAD1/5/8 protein in SVOG cells (P cell culture system, and may not reflect a realistic intra-ovarian environment. Our results suggested that BMP2 may be involved in the local modulation of cell

  11. [Construction of pGL3-SM22-SCAP (D443N) eukaryotic expression vector and its expression in CHO cells].

    Science.gov (United States)

    Wang, Yuanyuan; Hu, Jieli; Cui, Jing; Huang, Ailong; Ruan, Xiongzhong; Chen, Yaxi

    2010-01-01

    The experiment was designed to investigate the function of SREBP cleavage-activating protein (SCAP) mutant (D443N) by constructing an eukaryotic expressive vector using a smooth muscle specific promoter SM22 (pGL3-SM22-SCAP(D443N)). SM22 promoter (pSM22) was amplified from genome DNA of mice by nested PCR, and then cloned into pMD-T vector. The SM22 promoter fragment released from the vector by Kpn I and Hind III digestion was sub-cloned into pGL3-control-Luc vector, to form pGL3-SM22-Luc. The activity of pSM22 in human vascular smooth muscle cells (VSMCs) was tested using Dual-Luciferase Reporter System. SCAP(D443) mutant amplified from plasmid pTK-HSV-SCAP(D443N) and pSM22 from mice liver were cloned into pGL3-control vector to construct pGL3-SM22-SCAP(D443N) which was transfected into Chinese hamster ovary cells (CHO) to test SCAP(D443) expression by real-time PCR and Western blot. The sequence and construction of pGL3-SM22-SCAP(D443N) were correct. SM22 promoter activity initiated the expression of luciferase in VSMCs and also drove SCAP(D443) expression in transfected CHO cells. The pGL3-SM22-SCAP(D443N) eukaryotic expression vector was successfully constructed and the recombinant vector provides a powerful approach in investigating the function and regulation of SCAP and also in producing vascular smooth muscle specific SCAP transgenic mice.

  12. Enamiku sakslaste uriinis leidub glüfosaadijääke

    Index Scriptorium Estoniae

    2016-01-01

    Toidu ja joogiga satuvad glüfosaadid paratamatult inimeste organismi. Sellisele järeldusele jõuti Heinrich Böll Foundationi korraldatud 2009 sakslase uuringus, kus osalejate uriinist leiti glüfosaadijääke 99,6% juhtudel

  13. Bases anatómicas da cirurgia da glândula tiróide

    OpenAIRE

    Melo, Catarina José Monteiro Campos de

    2011-01-01

    Trabalho final de mestrado integrado em Medicina área cientifica de Anatomia, apresentado á Faculdade de Medicina da Universidade de Coimbra Introdução: É indispensável ter um conhecimento profundo da Morfologia da glândula tiróide, para minimizar a incidência de complicações suscitadas pela tiroidectomia, nomeadamente a lesão das glândulas paratiróides e dos nervos laríngeos. Objectivos: Revisão teórica sobre a Anatomia normal da glândula tiróide e das estruturas com ela relacionadas d...

  14. Tumor glômico endobrônquico com atelectasia de lobo superior direito

    OpenAIRE

    Azevedo-Pereira,Artur Eugênio de; Rigueiro,Moacyr Pezati; Abrão,Fernando Conrado

    2010-01-01

    Tumores glômicos são raras neoplasias benignas de tecidos moles. Relatamos o caso de um paciente de 32 anos de idade que apresentava atelectasia em lobo superior direito devido a um tumor endobrônquico. O paciente foi submetido a lobectomia superior direita e broncoplastia em cunha. O diagnóstico patológico foi de tumor glômico endobrônquico. Até onde sabemos, essa apresentação com atelectasia em lobo superior direito nunca foi relatada. O tumor glômico deve ser considerado no diagnóstico dif...

  15. Antibacterial activity and cytocompatibility of an implant coating consisting of TiO2 nanotubes combined with a GL13K antimicrobial peptide.

    Science.gov (United States)

    Li, Tao; Wang, Na; Chen, Su; Lu, Ran; Li, Hongyi; Zhang, Zhenting

    2017-01-01

    Prevention of implant-associated infections at an early stage of surgery is highly desirable for the long-term efficacy of implants in dentistry and orthopedics. Infection prophylaxis using conventional antibiotics is becoming less effective due to the development of bacteria resistant to multiple antibiotics. An ideal strategy to conquer bacterial infections is the local delivery of antibacterial agents. Therefore, antimicrobial peptide (AMP) eluting coatings on implant surfaces is a promising alternative. In this study, the feasibility of utilizing TiO2 nanotubes (TNTs), processed using anodization, as carriers to deliver a candidate AMP on titanium surfaces for the prevention of implant-associated infections is assessed. The broad-spectrum GL13K (GKIIKLKASLKLL-CONH2) AMP derived from human parotid secretory protein was selected and immobilized to TNTs using a simple soaking technique. Field emission scanning electron microscope, X-ray diffraction, Fourier transform infrared spectroscopy, and liquid chromatography-mass spectrometry analyses confirmed the successful immobilization of GL13K to anatase TNTs. The drug-loaded coatings demonstrated a sustained and slow drug release profile in vitro and eradicated the growth of Fusobacterium nucleatum and Porphyromonas gingivalis within 5 days of culture, as assessed by disk-diffusion assay. The GL13K-immobilized TNT (GL13K-TNT) coating demonstrated greater biocompatibility, compared with a coating produced by incubating TNTs with equimolar concentrations of metronidazole. GL13K-TNTs produced no observable cytotoxicity to preosteoblastic cells (MC3T3-E1). The coating may also have an immune regulatory effect, in support of rapid osseointegration around implants. Therefore, the combination of TNTs and AMP GL13K may achieve simultaneous antimicrobial and osteoconductive activities.

  16. Comparison of Physics Frameworks for WebGL-Based Game Engine

    Directory of Open Access Journals (Sweden)

    Yogya Resa

    2014-03-01

    Full Text Available Recently, a new technology called WebGL shows a lot of potentials for developing games. However since this technology is still new, there are still many potentials in the game development area that are not explored yet. This paper tries to uncover the potential of integrating physics frameworks with WebGL technology in a game engine for developing 2D or 3D games. Specifically we integrated three open source physics frameworks: Bullet, Cannon, and JigLib into a WebGL-based game engine. Using experiment, we assessed these frameworks in terms of their correctness or accuracy, performance, completeness and compatibility. The results show that it is possible to integrate open source physics frameworks into a WebGLbased game engine, and Bullet is the best physics framework to be integrated into the WebGL-based game engine.

  17. Comparison of Physics Frameworks for WebGL-Based Game Engine

    Science.gov (United States)

    Yogya, Resa; Kosala, Raymond

    2014-03-01

    Recently, a new technology called WebGL shows a lot of potentials for developing games. However since this technology is still new, there are still many potentials in the game development area that are not explored yet. This paper tries to uncover the potential of integrating physics frameworks with WebGL technology in a game engine for developing 2D or 3D games. Specifically we integrated three open source physics frameworks: Bullet, Cannon, and JigLib into a WebGL-based game engine. Using experiment, we assessed these frameworks in terms of their correctness or accuracy, performance, completeness and compatibility. The results show that it is possible to integrate open source physics frameworks into a WebGLbased game engine, and Bullet is the best physics framework to be integrated into the WebGL-based game engine.

  18. Cycloplegic effects of 0.5g/L tropicamide in various low myopia age groups

    National Research Council Canada - National Science Library

    Hai-Rong Zhang; Hong-Xing Diao; Xiang Chen

    2014-01-01

    AIM: To investigated the refraction and cycloplegic effects of 0.5g/L tropicamide eye drops in various low myopia age groups, and provide clinical guidelines for proper usage of cyclopegic agent in different population. METHODS...

  19. Prinsessen og det hele kongerige. Christian IX og det glücksborgske kongehus

    DEFF Research Database (Denmark)

    Olden-Jørgensen, Sebastian

    Det glücksborgske kongehus' begyndelse og udvikling analyseres med særlig vægt på begreberne nærhed og distance samt welfare monarchy.......Det glücksborgske kongehus' begyndelse og udvikling analyseres med særlig vægt på begreberne nærhed og distance samt welfare monarchy....

  20. Continuum limit of gl(M vertical stroke N) spin chains

    Energy Technology Data Exchange (ETDEWEB)

    Candu, Constantin [Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Gruppe Theorie

    2011-03-15

    We study the spectrum of an integrable antiferromagnetic Hamiltonian of the gl(M vertical stroke N) spin chain of alternating fundamental and dual representations. After extensive numerical analysis, we identify the vacuum and low lying excitations and with this knowledge perform the continuum limit, while keeping a finite gap. All antiferromagnetic gl(n+N vertical stroke N) spin chains with n>0 and N{ne}0 are shown to possess in the continuum limit 2n-2 multiplets of massive particles which scatter with gl(n) Gross-Neveu like S-matrices, namely their eigenvalues do not depend on N. We argue that the continuum theory is the gl(M vertical stroke N) Gross-Neveu model, that is the massive deformation of the gl(M vertical stroke N){sub 1} Wess-Zumino-Witten model. As we can see ion the example of gl(2m vertical stroke 1) spin chains, the full particle spectrum is much richer. Our analysis suggests that for a complete characterization of the latter it is not enough to restrict to large volume calculations, as we do in this work. (orig.)

  1. Aberrant cryptic responsiveness of the pCAT 3- and pGL3-promoter reporter vectors.

    Science.gov (United States)

    Giannakis, G; Edmondson, S R; Favaloro, J M; Zajac, J D; Greenland, K J

    2003-08-01

    Transfection analyses are an informative method to assess the activity of specific promoter or enhancer elements in mammalian cells. Commercially available reporter vectors can be extremely useful investigative tools for such studies. This study reports that the pCAT 3- and pGL3-promoter vectors display cryptic responsiveness to androgens when they contain a DNA insert, while the empty vector, a commonly used negative control, is nonresponsive. Our studies initially aimed to characterize novel androgen-responsive DNA sequences in human genomic DNA through transactivational analyses. An isolated DNA fragment, designated ARC-3, contained three putative androgen response element "half-sites" and was androgen-responsive when cloned into the pCAT3-promoter vector. While we originally believed this to be a novel enhancer element, subsequent analyses of this clone revealed that this vector displays cryptic activity in the presence of an androgen. This was confirmed by cloning several unrelated DNA fragments that did not contain any known classic response elements into the pCAT3-promoter vector, all of which were found to be responsive. The empty vector (negative control) was again nonresponsive. The ARC-3 DNA fragment was also weakly responsive to stimulation when cloned into the pGL3-promotor vector, which is identical to the pCAT3-promoter vector, with the exception of an intron located 5' of the chloramphenicol acetyltransferase gene, and the reporter genes. This work demonstrates that both the pCAT3- and pGL3-promoter vectors are inappropriate to assess androgen-responsive enhancers and emphasizes the importance of the careful selection of reporter vectors and controls when conducting transactivational analysis.

  2. Comparison of the responsiveness of the pGL3 and pGL4 luciferase reporter vectors to steroid hormones.

    Science.gov (United States)

    Dougherty, Dawne C; Sanders, Michel M

    2005-08-01

    The ovalbumin gene (Ov) gene is responsive to estrogen, glucocorticoid, androgen, and progesterone. In our efforts to characterize the regulation of the Ov gene by steroid hormones, we have repeatedly observed that many vector backbones and promoters are responsive to steroids. In order to determine which vectors are most suitable for these types of analyses, vectors from Promega's pGL3 and newly engineered pGL4 Dual-Luciferase Reporter Assay System were tested with both estrogen and/or corticosterone. The results confirmed that both series are induced by glucocorticoids in transient transfections of primary oviduct tubular gland cells, which contain glucocorticoid receptors, but not in MCF-7 cells, which do not. Modest effects that were dependent upon backbone and promoter context were observed with both series of vectors with estrogen. Thus, use of these vectors for experiments analyzing the effects of steroid hormones, especially glucocorticoids, should be done with caution. However the new pGL4 series does have some advantages over the older series, and a comparison of transcription factor binding sites is reported.

  3. Evolución y desarrollo de la glándula anular de Drosophila Melanogaster

    OpenAIRE

    Sánchez Higueras, Carlos

    2017-01-01

    Programa de Doctorado en Biotecnología, Ingeniería y Tecnología Química Durante el crecimiento de los artrópodos, la presencia de un exoesqueleto, obliga al animal a realizar varias mudas. En los insectos las mudas y la metamorfosis están reguladas por la hormona juvenil secretada por el corpora allata y la ecdisona secretada por la glándula protorácica. En los dípteros, ambas glándulas están asociadas en un órgano endocrino, la glándula anular. En las últimas décadas se ha ...

  4. Estudo morfológico da glândula pineal no cão

    OpenAIRE

    Lucas Alécio Gomes

    2003-01-01

    A glândula pineal animal por muito tempo foi uma estrutura “esquecida” ou considerada de pouca importância para muitos estudiosos ligados à anatomia. Após a descoberta da melatonina, hormônio por ela secretado novas pesquisas e perspectivas surgiram. Neste trabalho, efetuamos estudo morfológico desta glândula no cão, mediante análise macroscópica, mesoscópica e microscópica (microscopia de luz). Para este estudo, foram utilizados 20 cães. A glândula pineal revelou um formato variando de cônic...

  5. Game and Graphics Programming for iOS and Android with OpenGL ES 20

    CERN Document Server

    Marruchi-Foino, Romain; Semko, Roman

    2012-01-01

    Develop graphically sophisticated apps and games today! The smart phone app market is progressively growing, and there is new market gap to fill that requires more graphically sophisticated applications and games. Game and Graphics Programming for iOS and Android with OpenGL ES 2.0 quickly gets you up to speed on understanding how powerful OpenGL ES 2.0 technology is in creating apps and games for amusement and effectiveness. Leading you through the development of a real-world mobile app with live code, this text lets you work with all the best features and tools that Open GL ES 2.0 has to off

  6. WebGL and web audio software lightweight components for multimedia education

    Science.gov (United States)

    Chang, Xin; Yuksel, Kivanc; Skarbek, Władysław

    2017-08-01

    The paper presents the results of our recent work on development of contemporary computing platform DC2 for multimedia education usingWebGL andWeb Audio { the W3C standards. Using literate programming paradigm the WEBSA educational tools were developed. It offers for a user (student), the access to expandable collection of WEBGL Shaders and web Audio scripts. The unique feature of DC2 is the option of literate programming, offered for both, the author and the reader in order to improve interactivity to lightweightWebGL andWeb Audio components. For instance users can define: source audio nodes including synthetic sources, destination audio nodes, and nodes for audio processing such as: sound wave shaping, spectral band filtering, convolution based modification, etc. In case of WebGL beside of classic graphics effects based on mesh and fractal definitions, the novel image processing analysis by shaders is offered like nonlinear filtering, histogram of gradients, and Bayesian classifiers.

  7. 3D-kaupungin suunnittelu ja toteutus osana WebGL-multimediasovellusta

    OpenAIRE

    Sissala, Erik

    2015-01-01

    Insinöörityön tarkoituksena oli suunnitella ja toteuttaa 3D-kaupunki osana interaktiivista multimediasovellusta, joka hyödyntää WebGL-tekniikkaa. WebGL-tekniikan avulla verkkoselaimiin saadaan 3D-grafiikkaa ilman ylimääräisiä liitännäisiä. Insinöörityön toimeksiantaja oli suomalainen hissiyhtiö. Työ lähti liikkeelle toimeksiantajan tarpeesta saada esittelytilaansa enemmän interaktiivisuutta, mielenkiintoista sisältöä ja modernia teknologiaa. Entinen esittelytilan si...

  8. Efecto de la radioterapia en glándulas salivales accesorias.

    OpenAIRE

    Pérez Fernández, Carmen Aránzazu

    2005-01-01

    RESUMEN El objetivo de este trabajo es estudiar las alteraciones que las radiaciones ionizantes producen sobre las glándulas salivales accesorias labiales. En nuestro estudio se seleccionaron sujetos diagnosticados de un tumor maligno de cabeza y cuello que iban a recibir radioterapia dentro de su tratamiento, incluyendo los labios dentro del campo de irradiación. Se evaluó la secreción salival y las alteraciones morfológicas de las glándulas en el momento del diagnóstico...

  9. Tumores glômicos do osso temporal: relato de 6 casos

    Directory of Open Access Journals (Sweden)

    Walter Oleschko Arruda

    1989-03-01

    Full Text Available Os autores apresentam os achados clínicos, radiológicos e anátomo-patologicos de 6 casos de tumor glômico de osso temporal. Cinco pacientes eram do sexo feminino; a idade variava de 22 a 76 anos (média 48 anos. As manifestações clínicas predominantes foram hipoacusia, zumbido e achados otoscópicos sugestivos do diagnóstico. São revistos e discutidos vários aspectos relacionados à origem e fisiopatogênese dos tumores glômicos, assim como seu diagnóstico e tratamento.

  10. O darwinismo nas Conferências Populares da Glória Darwinism in the Popular Conferences in the Glória district of Rio de Janeiro

    Directory of Open Access Journals (Sweden)

    Karoline Carula

    2008-01-01

    Full Text Available Este artigo aborda o darwinismo exposto, entre 1875 e 1880, nas Conferências Populares da Glória - preleções públicas realizadas na escola da Freguesia da Glória, no Rio de Janeiro. As conferências que tiveram as idéias darwinistas como temática foram recebidas e repercutidas na imprensa de maneira polêmica. Apresenta-se a concepção sobre o darwinismo do médico Miranda Azevedo, o primeiro, mas não o único, a discursar sobre o tema. Com relação às conferências desse médico, analisa-se a aplicabilidade social do darwinismo por ele defendida, e as discussões na imprensa sobre a propagação do darwinismo no Brasil e sobre a possível origem símia do homem. Por fim, analisam-se as preleções de outros conferencistas que ou versaram sobre o tema mais diretamente ou perpassaram o assunto.In this paper I examine the Darwinism that transpired, between 1875 and 1880, during the Popular Conferences in the Gloria district - public conferences that took place at the Freguesia da Glória school, in Rio de Janeiro. The Conferences that had Darwinism as a subject were received and reverberated in the press polemically. I present the conception of Darwinism held by doctor Miranda Azevedo, the first, but not the only speaker, to discuss the subject. Concerning this doctor's conferences, I analyze the social application of Darwinism defended by him, and the discussions in the press about the spread of Darwinism in Brazil, and the possible simian origin of man. Finally, I talk about other lecturers' talks that either directly broached the subject or mentioned it in passing.

  11. The detection of a discrete outflow from the young stellar object GL 490

    Science.gov (United States)

    Mitchell, G. F.; Allen, M.; Beer, R.; Dekany, R.; Huntress, W.

    1988-01-01

    A high-resolution (0.059/cm) M-band spectrum has been obtained of the embedded young stellar object GL490. The spectrum shows interstellar absorption in the fundamental vibrational band, v = 1-0, of (C-12)O. Two strong and narrow (10 km/s) velocity components are present. One, at the velocity of GL490 (vLSR = -16 km/s), is likely gas in the molecular cloud within which GL490 is embedded. The other component is blueshifted by 13 km/s relative to GL490. An observation of emission from the J = 3-2 transition of HCO(+) using a 20-arcsec beam supports the view that the blueshifted gas is near the central object. The -29-km/s feature is interpreted as a recently ejected shell. It is conjectured that the extended outflows of cold molecular gas seen by millimeter CO emission observations are driven by sporadic outbursts rather than by continuous flows from the central object.

  12. Data of evolutionary structure change: 1SZOH-2J5GL [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available Chain>1SZOH RLERD-GGVLL e>EEEEE- EEEucture...HRDENGILE >EEEEE EEE> ATOM 22372...ryIDChain>1SZOH DFTSF-NLGTP cture...2J5GL DFPSLGDVTNP > H> EGGG - H> ATOM 14157 CA ASP H 78 60.734 94.793 25.324 1.00

  13. Learning Three.js the JavaScript 3D library for WebGL

    CERN Document Server

    Dirksen, Jos

    2015-01-01

    If you know JavaScript and want to start creating 3D graphics that run in any browser, this book is a great choice for you. You don't need to know anything about math or WebGL; all that you need is general knowledge of JavaScript and HTML.

  14. RIM+ "STYLE GUIDE" EN CODEER REGELS voor Ingres Windows4GL

    NARCIS (Netherlands)

    van der Maas CWM; Slootweg J; Knol OM; Laan WPM; Baart R; Bruinsma PH

    1993-01-01

    The LAE-department (Laboratory for Waste Materials and Emissions) is developing an Environmental Information and Planning Model (RIM+). This is a collection of applications based on an Ingres database. For the development of the applications Windows4GL is used in combination with embedded SQL for

  15. Morfologia da glândula pineal de gambás (Didelphis sp

    Directory of Open Access Journals (Sweden)

    Celina Almeida Furlanetto Mançanares

    2007-06-01

    Full Text Available A glândula pineal deve ser analisada e estudada em animais da fauna brasileira, para que dados da pesquisa básica possam ser aplicados em novas técnicas de manejo reprodutivo destes animais, inclusive em cativeiro, face à íntima relação deste órgão fotorreceptor com o ciclo reprodutivo. Para este estudo, foram utilizados 10 gambás (Didelphis sp, provenientes do Departamento de Anatomia da USP e da UNIFEOB, já mortos e fixados. Nenhum animal foi submetido a situações de dor/sofrimento e ao sacrifício de sua vida. A glândula pineal foi encontrada em todos animais estudados e apresentou-se com diminutas dimensões, não sendo possível, portanto descrever-lhe características macroscópicas. Através da análise microscópica pudemos localizar a glândula no espaço correspondente ao plano mediano, em relação ao encéfalo, rostral e dorsalmente aos colículos rostrais, ventralmente aos hemisférios cerebrais e caudalmente à comissura habenular. Consiste de uma evaginação do teto do diencéfalo e mostra-se em forma de "U" invertido. Comparativamente a características de glândulas pineais de outras espécies animais, a do Didelphis genus, que estudamos, revela peculiaridades tanto em relação ao seu tamanho, apenas perceptível microscopicamente, quanto ao fato de apresentar células semelhantes às secretoras, dispersas também em áreas vizinhas. Tais peculiaridades motivam reflexões sobre o papel funcional da glândula, na espécie considerada.

  16. Colgajos de perforantes de arteria glútea superior y arteria glútea inferior Gluteal artery perforator flaps: S-GAP and I-GAP

    Directory of Open Access Journals (Sweden)

    R. J. Allen

    2006-12-01

    Full Text Available En la actualidad la transferencia de tejido ha evolucionado hasta el nivel de los colgajos de perforantes, los cuales aportan tejido formado por piel y grasa, con la mínima morbilidad a nivel de la zona donante. Los colgajos de perforantes de la región glútea, de arteria glutea superior (S-GAP y de arteria glutea inferior (I-GAP, han ganado popularidad debido a su fiabilidad, preservación del músculo, versatilidad en el diseño sin comprometer otras alternativas reconstructivas y por su baja morbilidad. Se analizarán en este artículo de forma detallada la anatomía, técnica quirúrgica e indicaciones específicas de estos colgajos de perforantes de la región glútea.The evolution of free tissue transfer has progressed to the level of the perforator flaps. They allow the transfer of the patient’s own skin and fat in a reliable way with minimal donor-site morbidity. Nowadays the gluteal artery perforator flaps, superior (S-GAP and inferior (I-GAP, have gained popularity due to reliability, preservation of the muscle, versatility in flap design without restricting other flap options, and lower donor-site morbidity. Anatomy of the gluteal region, flap raising technique and specific indications will be well-described.

  17. Piglet nasal microbiota at weaning may influence the development of Glässer's disease during the rearing period.

    Science.gov (United States)

    Correa-Fiz, Florencia; Fraile, Lorenzo; Aragon, Virginia

    2016-05-26

    The microbiota, the ensemble of microorganisms on a particular body site, has been extensively studied during the last few years, and demonstrated to influence the development of many diseases. However, these studies focused mainly on the human digestive system, while the populations in the respiratory tract have been poorly assessed, especially in pigs. The nasal mucosa of piglets is colonized by an array of bacteria, many of which are unknown. Among the early colonizers, Haemophilus parasuis also has clinical importance, since it is also the etiological agent of Glässer's disease. This disease produces economical losses in all the countries with pig production, and the factors influencing its development are not totally understood. Hence, the purpose of this work was to characterize the nasal microbiota composition of piglets, and its possible role in Glässer's disease development. Seven farms from Spain (4 with Glässer's disease and 3 control farms without any respiratory disease) and three farms from UK (all control farms) were studied. Ten piglets from each farm were sampled at 3-4 weeks of age before weaning. The total DNA extracted from nasal swabs was used to amplify the 16S RNA gene for sequencing in Illumina MiSeq. Sequencing data was quality filtered and analyzed using QIIME software. The diversity of the nasal microbiota was low in comparison with other body sites, showing a maximum number of operational taxonomic units (OTUs) per pig of 1,603, clustered in five phyla. Significant differences were found at various taxonomical levels, when the microbiota was compared regarding the farm health status. Healthy status was associated to higher species richness and diversity, and UK farms demonstrated the highest diversity. The composition of the nasal microbiota of healthy piglets was uncovered and different phylotypes were shown to be significantly altered in animals depending on the clinical status of the farm of origin. Several OTUs at genus level were

  18. Targeting Protein Kinase CK2: Evaluating CX-4945 Potential for GL261 Glioblastoma Therapy in Immunocompetent Mice

    Science.gov (United States)

    Ferrer-Font, Laura; Villamañan, Lucia; Arias-Ramos, Nuria; Vilardell, Jordi; Plana, Maria; Ruzzene, Maria; Pinna, Lorenzo A.; Itarte, Emilio; Arús, Carles; Candiota, Ana Paula

    2017-01-01

    Glioblastoma (GBM) causes poor survival in patients even with aggressive treatment. Temozolomide (TMZ) is the standard chemotherapeutic choice for GBM treatment but resistance always ensues. Protein kinase CK2 (CK2) contributes to tumour development and proliferation in cancer, and it is overexpressed in human GBM. Accordingly, targeting CK2 in GBM may benefit patients. Our goal has been to evaluate whether CK2 inhibitors (iCK2s) could increase survival in an immunocompetent preclinical GBM model. Cultured GL261 cells were treated with different iCK2s including CX-4945, and target effects evaluated in vitro. CX-4945 was found to decrease CK2 activity and Akt(S129) phosphorylation in GL261 cells. Longitudinal in vivo studies with CX-4945 alone or in combination with TMZ were performed in tumour-bearing mice. Increase in survival (p < 0.05) was found with combined CX-4945 and TMZ metronomic treatment (54.7 ± 11.9 days, n = 6) when compared to individual metronomic treatments (CX-4945: 24.5 ± 2.0 and TMZ: 38.7 ± 2.7, n = 6) and controls (22.5 ± 1.2, n = 6). Despite this, CX-4945 did not improve mice outcome when administered on every/alternate days, either alone or in combination with 3-cycle TMZ. The highest survival rate was obtained with the metronomic combined TMZ+CX-4945 every 6 days, pointing to the participation of the immune system or other ancillary mechanism in therapy response. PMID:28208677

  19. Targeting Protein Kinase CK2: Evaluating CX-4945 Potential for GL261 Glioblastoma Therapy in Immunocompetent Mice

    Directory of Open Access Journals (Sweden)

    Laura Ferrer-Font

    2017-02-01

    Full Text Available Glioblastoma (GBM causes poor survival in patients even with aggressive treatment. Temozolomide (TMZ is the standard chemotherapeutic choice for GBM treatment but resistance always ensues. Protein kinase CK2 (CK2 contributes to tumour development and proliferation in cancer, and it is overexpressed in human GBM. Accordingly, targeting CK2 in GBM may benefit patients. Our goal has been to evaluate whether CK2 inhibitors (iCK2s could increase survival in an immunocompetent preclinical GBM model. Cultured GL261 cells were treated with different iCK2s including CX-4945, and target effects evaluated in vitro. CX-4945 was found to decrease CK2 activity and Akt(S129 phosphorylation in GL261 cells. Longitudinal in vivo studies with CX-4945 alone or in combination with TMZ were performed in tumour-bearing mice. Increase in survival (p < 0.05 was found with combined CX-4945 and TMZ metronomic treatment (54.7 ± 11.9 days, n = 6 when compared to individual metronomic treatments (CX-4945: 24.5 ± 2.0 and TMZ: 38.7 ± 2.7, n = 6 and controls (22.5 ± 1.2, n = 6. Despite this, CX-4945 did not improve mice outcome when administered on every/alternate days, either alone or in combination with 3-cycle TMZ. The highest survival rate was obtained with the metronomic combined TMZ+CX-4945 every 6 days, pointing to the participation of the immune system or other ancillary mechanism in therapy response.

  20. Glándula hipofisaria: morfología normal y patológica

    OpenAIRE

    Cónsole-Avegliano, Gloria Miriam

    2017-01-01

    La obra Glándula hipofisaria: morfología normal y patológica integra y actualiza las investigaciones morfológicas normales y patológicas del eje hipotálamo-hipofisario desarrolladas en el Laboratorio de Investigaciones Neuroendocrinas de la Cátedra B de Citología, Histología y Embriología de la Facultad de Ciencias Médicas de la Universidad Nacional de La Plata (período 1990-2016). Los estudios morfológicos de la glándula pituitaria aportan una revisión de la embriología molecular, la hist...

  1. iPhone 3D Programming Developing Graphical Applications with OpenGL ES

    CERN Document Server

    Rideout, Philip

    2010-01-01

    What does it take to build an iPhone app with stunning 3D graphics? This book will show you how to apply OpenGL graphics programming techniques to any device running the iPhone OS -- including the iPad and iPod Touch -- with no iPhone development or 3D graphics experience required. iPhone 3D Programming provides clear step-by-step instructions, as well as lots of practical advice, for using the iPhone SDK and OpenGL. You'll build several graphics programs -- progressing from simple to more complex examples -- that focus on lighting, textures, blending, augmented reality, optimization for pe

  2. Experimental immunotherapy for malignant glioma: lessons from two decades of research in the GL261 model.

    Science.gov (United States)

    Maes, Wim; Van Gool, Stefaan W

    2011-02-01

    Nearly twenty years of experimental immunotherapy for malignant glioma yielded important insights in the mechanisms governing glioma immunology. Still considered promising, it is clear that immunotherapy does not on its own represent the magic bullet in glioma therapy. In this review, we summarize the major immunotherapeutic achievements in the mouse GL261 glioma model, which has emerged as the gold standard syngeneic model for experimental glioma therapy. Gene therapy, monoclonal antibody treatment, cytokine therapy, cell transfer strategies and dendritic cell therapy were hereby considered. Apart from the considerable progress made in understanding glioma immunology in this model, we also addressed its most pertinent issues and shortcomings. Despite these, the GL261 model will remain indispensable in glioma research since it is a fast, highly reproducible and easy-to-establish model system.

  3. WebGL-enabled 3D visualization of a Solar Flare Simulation

    Science.gov (United States)

    Chen, A.; Cheung, C. M. M.; Chintzoglou, G.

    2016-12-01

    The visualization of magnetohydrodynamic (MHD) simulations of astrophysical systems such as solar flares often requires specialized software packages (e.g. Paraview and VAPOR). A shortcoming of using such software packages is the inability to share our findings with the public and scientific community in an interactive and engaging manner. By using the javascript-based WebGL application programming interface (API) and the three.js javascript package, we create an online in-browser experience for rendering solar flare simulations that will be interactive and accessible to the general public. The WebGL renderer displays objects such as vector flow fields, streamlines and textured isosurfaces. This allows the user to explore the spatial relation between the solar coronal magnetic field and the thermodynamic structure of the plasma in which the magnetic field is embedded. Plans for extending the features of the renderer will also be presented.

  4. Research and exploitation of CNC laser manufacturing online monitoring system based on OpenGL

    Science.gov (United States)

    Wang, Gang; Hu, Shengsun; Wang, Mingjian; Ding, Wei; Zhao, Jie

    2010-12-01

    CNC manufacturing online monitoring technology is a significant method to improve the processing quality and achieve an important part of intelligent processing. Based on OpenGL 3D graphics technology, a Computer Numerical Control (CNC ) Laser Manufacturing 3D model is established; based on the model and the powerful ability of OpenGL, a CNC manufacturing on-line monitoring system is developed so as to achieve the ability of CNC remote real-time online monitoring in different working locations. Remote real-time online monitoring of different position of the CNC is realized. The online monitoring scope of the CNC is enlarged, with more flexibility to meet the demands of practical application, meanwhile the cost of hardware investment is greatly reduced.

  5. Perfluorocarbon emulsions radiosensitise brain tumors in carbogen breathing mice with orthotopic GL261 gliomas

    Science.gov (United States)

    Feldman, Lisa A.; Fabre, Marie-Sophie; Grasso, Carole; Reid, Dana; Broaddus, William C.; Lanza, Gregory M.; Spiess, Bruce D.; Garbow, Joel R.; McConnell, Melanie J.

    2017-01-01

    Background Tumour hypoxia limits the effectiveness of radiation therapy. Delivering normobaric or hyperbaric oxygen therapy elevates pO2 in both tumour and normal brain tissue. However, pO2 levels return to baseline within 15 minutes of stopping therapy. Aim To investigate the effect of perfluorocarbon (PFC) emulsions on hypoxia in subcutaneous and intracranial mouse gliomas and their radiosensitising effect in orthotopic gliomas in mice breathing carbogen (95%O2 and 5%CO2). Results PFC emulsions completely abrogated hypoxia in both subcutaneous and intracranial GL261 models and conferred a significant survival advantage orthotopically (Mantel Cox: p = 0.048) in carbogen breathing mice injected intravenously (IV) with PFC emulsions before radiation versus mice receiving radiation alone. Carbogen alone decreased hypoxia levels substantially and conferred a smaller but not statistically significant survival advantage over and above radiation alone. Conclusion IV injections of PFC emulsions followed by 1h carbogen breathing, radiosensitises GL261 intracranial tumors. PMID:28873460

  6. C.W.W. BeecherEl and G.L. BogveC

    African Journals Online (AJOL)

    MOI.-LUSCICIDAL CONS'I'i'I'UENTS OF THE STEM BARK. OF TETRAPLEURA TETRAPTERA. 0.13. Ngamapaa, N.R. Farnsworth*a, T.O. Hendersonb,. C.W.W. BeecherEl and G.L. BogveC. 8'LE-'rograirn for Collaborative Research in the Pharmaceutical Sciences,. College of Pharmacy University of Illinois at Chicago, Box ...

  7. Development and implementation of a propeller test capability for GL-10 "Greased Lightning" propeller design

    Science.gov (United States)

    Duvall, Brian Edward

    Interest in small unmanned aerial vehicles has increased dramatically in recent years. Hybrid vehicles which allow forward flight as a fixed wing aircraft and a true vertical landing capability have always had applications. Management of the available energy and noise associated with electric propeller propulsion systems presents many challenges. NASA Langley has developed the Greased Lightning 10 (GL-10) vertical takeoff, unmanned aerial vehicle with ten individual motors and propellers. All are used for propulsion during takeoff and contribute to acoustic noise pollution which is an identified nuisance to the surrounding users. A propeller test capability was developed to gain an understanding of how the noise can be reduced while meeting minimum thrust requirements. The designed propeller test stand allowed for various commercially available propellers to be tested for potential direct replacement of the current GL-10 propellers and also supported testing of a newly designed propeller provided by the Georgia Institute of Technology. Results from the test program provided insight as to which factors affect the noise as well as performance characteristics. The outcome of the research effort showed that the current GL-10 propeller still represents the best choice of all the candidate propellers tested.

  8. Patología quirúrgica de la glándula tiroides

    Directory of Open Access Journals (Sweden)

    Juan Oré

    2008-09-01

    Full Text Available Introducción: Debido a la gran diversidad de patología quirúrgica de la glándula tiroides y su alta frecuencia en nuestro medio, creemos conveniente presentar nuestra experiencia en esta patología. Objetivos: Conocer la incidencia de la patología quirúrgica de la glándula tiroides, según diagnóstico anatomopatológico, en pacientes operados. Diseño: Estudio retrospectivo, observacional y descriptivo. Lugar: Servicio de cirugía de cabeza y cuello, Hospital Dos de Mayo, Lima, Perú, hospital de enseñanza médica. Participantes: Pacientes operados de la glándula tiroides. Intervenciones: Se revisó las historias clínicas de pacientes operados de la glándula tiroides, desde enero de 1997 hasta diciembre de 2006. Principales medidas de resultados: Resultados anatomopatológicos en los especímenes de glándula tiroides. Resultados: Hubo 274 casos operados, de los cuales 81,4% del total correspondió al sexo femenino; 56,9% de los casos provenía de fuera de Lima y Callao, con edades que fluctuaban desde los 30 y 59 años. El tumor fue el signo predominante en 97,8% de los casos. La operación realizada más frecuente fue la tiroidectomía total (39,8%. La patología benigna representó 58% de los casos, con el adenoma folicular como principal patología benigna (23%; dentro de las neoplasias malignas (42% del total, 23,7% era carcinoma papilar. Conclusiones: La patología tumoral tiroidea presentó un marcado predominio en el sexo femenino, principalmente en los grupos etáreos de la 4a, 5a y 6a décadas. La patología glandular tiroidea fue en su mayoría benigna, y de la maligna, el carcinoma papilar. Con alto valor de especificidad (97,7% y valor predictivo positivo (95,4%, la biopsia de aspiración por aguja fina de tiroides continúa siendo el examen auxiliar más importante en el estudio del paciente con patología tumoral tiroidea.

  9. [Construction of pGL3-hTERT-tk recombinant plasmid and effect of PEG-PEI/Fe(3)0(4) Nano-magnetic fluid-tk/GCV on proliferation of gastric cancer cells BGC823 in vitro].

    Science.gov (United States)

    Buduwaili, Wu-shouer; Li, Ling; Zhang, Bang-chang

    2012-07-01

    To construct an hTERT promoter-controlled recombinant plasmid HSV-tk, and to investigate its expression in human gastric cancer cells BGC823 and its effect on proliferation of gastric cancer cells BGC823 in vitro. Recombinant plasmid pGL3-hTERT-tk and the corresponding reporter plasmid pGL3-hTERT-tk-Luc(+) were constructed by gene engineering. The recombinant plasmids were then transfected into gastric cancer cells BGC823 via PEG-PEI/Fe(3)0(4) magnetic nano-particles. Fluorescence microscope was used to observe the changes of cell morphology and the transfection efficiency. The expression of the target gene in gastric cancer cells BGC823 was detected by immunohistochemistry. MTT assay was used to evaluate the effect of HSV-tk on the proliferation of BGC823 cells. Normal hepatic cells L02 were used as controls in all the experiments. Recombinant plasmid pGL3-hTERT-tk was successfully constructed, and the length was 1100 bp. pGL3-control-tk-Luc(+), pGL3-basic-tk-Luc(+) and pGL3-hTERT-tk-Luc(+) all could effectively transfect BGC823 cells with high telomerase activity, with the transfection rate being(28.1±2.3)%. Immunohistochemistry study showed significant expression of HSV-tk gene in the cytoplasm of BGC823 cells. MTT showed that 4 days after transfection of pGL3-hTERT-tk, the proliferation of BGC823 was inhibited, and the A570 value was(0.254±0.011), significantly lower than that of L02 cells(0.322±0.013) and that of BGC823 transfected by pGL3-basic-tk (0.357±0.014)(all P<0.05). Magnetic nano-particles can transfect pGL3-hTERT-tk into BGC823 cells and the expression is achieved. PEG-PEI/Fe(3)0(4) magnetic nano-particles can significantly inhibit the proliferation of BGC823 and may become a potential biological agent for gene therapy of gastric cancer.

  10. Lipidomics investigation of reversal effect of glycyrrhizin (GL) towards lithocholic acid (LCA)-induced alteration of phospholipid profiles.

    Science.gov (United States)

    Han, Jing-Chun; Yu, Jian; Gao, Ya-Jie

    2014-12-01

    Glycyrrhizin (GL), the major ingredient isolated from licorice, exerts multiple pharmacological activities. To elucidate the protective mechanism of GL towards lithocholic acid (LCA)-induced liver toxicity using lipidomics. GL (200 mg/kg) dissolved in corn oil was treated intraperitoneally for 7 d. On the 4th day, 200 mg/kg LCA was used to treat mice (i.p., twice daily) for another 4 d. The protective role of GL towards LCA-induced liver toxicity was investigated through evaluating the liver histology and the activity of alanine transaminase (ALT). The complete lipid profile was employed using UFLC-Triple TOF MS-based lipidomics. Intraperitoneal (i.p.) administration of 200 mg/kg GL can significantly protect LCA-induced liver damage, indicated by alleviated histology alteration and prevention of the ALT elevation. Lipidomics analysis can well separate the control group from LCA-treated group, and three lipid components were major contributors, including LPC 16:0, LPC 18:0, and LPC 18:2. GL treatment can significantly prevent LCA-induced reduction of these three lipid compounds, providing a new explanation for GL's protection mechanism towards LCA-induced liver toxicity. The recent study highlights the importance of lipidomics in elucidating the therapeutic mechanism of herbs.

  11. Glândulas intramandibulares de Formicidae (Ponerinae, Myrmicinae): desenvolvimento e seus componentes químicos

    OpenAIRE

    Martins, Luiza Carla Barbosa

    2012-01-01

    As formigas são especialmente ricas em glândulas exócrinas que variam na sua natureza estrutural, química e complexidade funcional, e esta riqueza em glândulas exócrinas talvez seja o grande ponto de seu sucesso biológico. Este estudo focalizou as glândulas intramandibulares de formigas primitivas Ponerini (Ponerinae) e derivadas Attini (Myrmicinae) quanto ao desenvolvimento de formas imaturas, diferenciação entre castas e componentes químicos, contribuindo para a compreensão dos mecanismos...

  12. glUCModel: a monitoring and modeling system for chronic diseases applied to diabetes.

    Science.gov (United States)

    Hidalgo, J Ignacio; Maqueda, Esther; Risco-Martín, José L; Cuesta-Infante, Alfredo; Colmenar, J Manuel; Nobel, Javier

    2014-04-01

    Chronic patients must carry out a rigorous control of diverse factors in their lives. Diet, sport activity, medical analysis or blood glucose levels are some of them. This is a hard task, because some of these controls are performed very often, for instance some diabetics measure their glucose levels several times every day, or patients with chronic renal disease, a progressive loss in renal function, should strictly control their blood pressure and diet. In order to facilitate this task to both the patient and the physician, we have developed a web application for chronic diseases control which we have particularized to diabetes. This system, called glUCModel, improves the communication and interaction between patients and doctors, and eventually the quality of life of the former. Through a web application, patients can upload their personal and medical data, which are stored in a centralized database. In this way, doctors can consult this information and have a better control over patient records. glUCModel also presents three novelties in the disease management: a recommender system, an e-learning course and a module for automatic generation of glucose levels model. The recommender system uses Case Based Reasoning. It provides automatic recommendations to the patient, based on the recorded data and physician preferences, to improve their habits and knowledge about the disease. The e-learning course provides patients a space to consult information about the illness, and also to assess their own knowledge about the disease. Blood glucose levels are modeled by means of evolutionary computation, allowing to predict glucose levels using particular features of each patient. glUCModel was developed as a system where a web layer allows the access of the users from any device connected to the Internet, like desktop computers, tablets or mobile phones. Copyright © 2014 Elsevier Inc. All rights reserved.

  13. Einsatz der Laserinduzierten Glühtechnik zur Charakterisierung von Nanopartikeln unter produktionsnahen Bedingungen

    OpenAIRE

    Dankers, Stefan

    2005-01-01

    Für die Bestimmung der Primärteilchengröße von Nanopartikeln wird im Rahmen dieser Arbeit die laserinduzierte Glühtechnik (Laser-Induced Incandescence - LII) eingesetzt. Diese ist ein optisches Messverfahren, das auf der Aufheizung der nanoskaligen Teilchen durch einen Laserpuls und der anschließenden Detektion der Wärmestrahlung beruht. Durch Untersuchung des Abkühlverhaltens der Teilchen kann deren Primärpartikelgröße ermittelt werden, da kleine Teilchen schneller abkühlen. Es wird die Anwe...

  14. Interactive WebGL-based 3D visualizations for EAST experiment

    Energy Technology Data Exchange (ETDEWEB)

    Xia, J.Y., E-mail: jyxia@ipp.ac.cn [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, Anhui (China); University of Science and Technology of China, Hefei, Anhui (China); Xiao, B.J. [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, Anhui (China); University of Science and Technology of China, Hefei, Anhui (China); Li, Dan [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, Anhui (China); Wang, K.R. [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, Anhui (China); University of Science and Technology of China, Hefei, Anhui (China)

    2016-11-15

    Highlights: • Developing a user-friendly interface to visualize the EAST experimental data and the device is important to scientists and engineers. • The Web3D visualization system is based on HTML5 and WebGL, which runs without the need for plug-ins or third party components. • The interactive WebGL-based 3D visualization system is a web-portal integrating EAST 3D models, experimental data and plasma videos. • The original CAD model was discretized into different layers with different simplification to enable realistic rendering and improve performance. - Abstract: In recent years EAST (Experimental Advanced Superconducting Tokamak) experimental data are being shared and analyzed by an increasing number of international collaborators. Developing a user-friendly interface to visualize the data, meta data and the relevant parts of the device is becoming more and more important to aid scientists and engineers. Compared with the previous virtual EAST system based on VRML/Java3D [1] (Li et al., 2014), a new technology is being adopted to create a 3D visualization system based on HTML5 and WebGL, which runs without the need for plug-ins or third party components. The interactive WebGL-based 3D visualization system is a web-portal integrating EAST 3D models, experimental data and plasma videos. It offers a highly interactive interface allowing scientists to roam inside EAST device and view the complex 3-D structure of the machine. It includes technical details of the device and various diagnostic components, and provides visualization of diagnostic metadata with a direct link to each signal name and its stored data. In order for the quick access to the device 3D model, the original CAD model was discretized into different layers with different simplification. It allows users to search for plasma videos in any experiment and analyze the video frame by frame. In this paper, we present the implementation details to enable realistic rendering and improve performance.

  15. Antike Gläser und versteinertes Elfenbein: Röntgenanalyse in der Kunst

    Science.gov (United States)

    Reiche, Ina; Radtke, Martin; Brouder, Christian

    2003-03-01

    Physikalische Methoden der Archäometrie liefern den Kunsthistorikern und Archäologen wichtige Informationen. Materialspezifische Größen geben Aufschluss über Alter, Authentizität, Herkunft und Herstellungstechniken sowie Alterserscheinungen von Gegenständen. Insbesondere die Röntgenanalyse hat in jüngster Zeit wertvolle Beiträge hierzu geliefert. Dabei spannt sich ein weiter Bogen von den Mysterien ägyptischer Augenschminke über die Herstellungstechnik römischer Gläser bis zur Authentizitätsuntersuchung mittelalterlicher Silberstiftzeichnungen.

  16. Ocorrência e morfometria de glândulas tegumentares abdominais em Apis mellifera L. (Hymenoptera, Apidae

    Directory of Open Access Journals (Sweden)

    Marcia Heloiza Vilas Boas Mota

    1988-07-01

    Full Text Available Apis mellifera L. apresenta três diferentes tipos de glândulas abdominais: (i glândulas tergais unicelulares, representadas por: a glândulas de Koschenikow, situadas no VIII tergo abdominal de operárias e rainhas: b glândulas de Nassanoff ou glândulas de cheiro, localizadas no VII tergo das operárias: c glândulas de Renner e Baumann, localizadas nos tergos III, IV e V das rainhas; (ii glândulas epiteliais, representadas pelas glândulas de cera das abelhas operárias; (iii glândulas associadas ao ferrão: a ácidas; b básicas, ocorrendo em ambas as castas. O estudo do ciclo de desenvolvimento das glândulas abdominais foi realizado por meio de técnicas morfométricas, que consistiram em medidas diretas das células e dos diâmetros de seus núcleos e cálculo posterior de suas respectivas áreas. A ocorrência e a morfometria mostraram correlações entre o ciclo de desenvolvimento e a função das glândulas e o comportamento das abelhas.Apis mellifera L. presents threww different types of abdominal glands: (i unicelular tergal glands, represented by: a the Koschenikow glands, situated in the VIII abdominal tergum of workers and queens; b the Nassanoff glands or scent glands located in the VII tergum of workers; c the Renner and Baumann glands located in the III, IV and V terga of the queens; (ii epithelial glands, represented by wax glands of worker bees; (iii glands associated to the sting: a acid; b basic glands, occurring in both castes. The study of the developmental cycle of the abdominal glands was done by means of morfometric tecniques which consisted in direct measurements of the cells and their nuclei diameters and further calculation of their respective areas: The occurrence and morphometric studies showed correlations between the developmental cycle and the glands function and bees behavior.

  17. bioWeb3D: an online webGL 3D data visualisation tool.

    Science.gov (United States)

    Pettit, Jean-Baptiste; Marioni, John C

    2013-06-07

    Data visualization is critical for interpreting biological data. However, in practice it can prove to be a bottleneck for non trained researchers; this is especially true for three dimensional (3D) data representation. Whilst existing software can provide all necessary functionalities to represent and manipulate biological 3D datasets, very few are easily accessible (browser based), cross platform and accessible to non-expert users. An online HTML5/WebGL based 3D visualisation tool has been developed to allow biologists to quickly and easily view interactive and customizable three dimensional representations of their data along with multiple layers of information. Using the WebGL library Three.js written in Javascript, bioWeb3D allows the simultaneous visualisation of multiple large datasets inputted via a simple JSON, XML or CSV file, which can be read and analysed locally thanks to HTML5 capabilities. Using basic 3D representation techniques in a technologically innovative context, we provide a program that is not intended to compete with professional 3D representation software, but that instead enables a quick and intuitive representation of reasonably large 3D datasets.

  18. Glässer's disease in piglets produced by intraperitoneal inoculation with Haemophilus parasuis.

    Science.gov (United States)

    Morozumi, T; Hiramune, T; Kobayashi, K

    1981-01-01

    Haemophilus parasuis was inoculated into the abdominal cavities of piglets. Its infective doses varied from 1.4 X 10(6) to 1.0 X 10(9) organisms. When inoculated with 1.0 X 10(9) or 1.6 X 10(8) organisms, piglets were affected with fibrinous polyserositis associated with arthritis and purulent meningitis. The three disorders were considered to be characteristic of Glässer's disease. Septicemia was also found in many of the infected piglets, some of which died within a few days after inoculation. No lesions were observed in piglets inoculated with 1.4 X 10(7) or 1.4 X 10(6) organisms. It was difficult to reisolate H.parasuis from lesions, especially those in the abdominal cavities of piglets killed on the 6th day after inoculation. Agglutinating and complement-fixing antibodies were present in the piglets with Glässer's syndrome examined on the 6th day after inoculation. Neither of them was detected in any piglet manifesting no clinical signs, except transient pyrexia.

  19. HTML5 games creating fun with HTML5, CSS3, and WebGL

    CERN Document Server

    Seidelin, Jacob

    2011-01-01

    Discover new opportunities for building 2D and 3D games with HTML5 The newest iteration of HTML, HTML5 can be used with JavaScript, CSS3, and WebGL to create beautiful, unique, engaging games that can be played on the web or mobile devices like the iPad or Android phones. Gone are the days where games were only possible with plugin technologies such as Flash and this cutting-edge book shows you how to utilize the latest open web technologies to create a game from scratch using Canvas, HTML5 Audio, WebGL, and WebSockets. You'll discover how to build a framework on which you will create your HTML5 game. Then each successive chapter covers a new aspect of the game, including user input, sound, multiplayer functionality, 2D and 3D graphics, and more. Aims directly at a new way to develop games for the web through the use of HTML5 Demonstrates how to make iOS and Android web apps Explains how to capture player input; create 3D graphics; and incorporate textures, lighting, and sound Guides you through the proce...

  20. Fast segmentation of satellite images using SLIC, WebGL and Google Earth Engine

    Science.gov (United States)

    Donchyts, Gennadii; Baart, Fedor; Gorelick, Noel; Eisemann, Elmar; van de Giesen, Nick

    2017-04-01

    Google Earth Engine (GEE) is a parallel geospatial processing platform, which harmonizes access to petabytes of freely available satellite images. It provides a very rich API, allowing development of dedicated algorithms to extract useful geospatial information from these images. At the same time, modern GPUs provide thousands of computing cores, which are mostly not utilized in this context. In the last years, WebGL became a popular and well-supported API, allowing fast image processing directly in web browsers. In this work, we will evaluate the applicability of WebGL to enable fast segmentation of satellite images. A new implementation of a Simple Linear Iterative Clustering (SLIC) algorithm using GPU shaders will be presented. SLIC is a simple and efficient method to decompose an image in visually homogeneous regions. It adapts a k-means clustering approach to generate superpixels efficiently. While this approach will be hard to scale, due to a significant amount of data to be transferred to the client, it should significantly improve exploratory possibilities and simplify development of dedicated algorithms for geoscience applications. Our prototype implementation will be used to improve surface water detection of the reservoirs using multispectral satellite imagery.

  1. A massive hypergiant star as the progenitor of the supernova SN 2005gl.

    Science.gov (United States)

    Gal-Yam, A; Leonard, D C

    2009-04-16

    Our understanding of the evolution of massive stars before their final explosions as supernovae is incomplete, from both an observational and a theoretical standpoint. A key missing piece in the supernova puzzle is the difficulty of identifying and studying progenitor stars. In only a single case-that of supernova SN 1987A in the Large Magellanic Cloud-has a star been detected at the supernova location before the explosion, and been subsequently shown to have vanished after the supernova event. The progenitor of SN 1987A was a blue supergiant, which required a rethink of stellar evolution models. The progenitor of supernova SN 2005gl was proposed to be an extremely luminous object, but the association was not robustly established (it was not even clear that the putative progenitor was a single luminous star). Here we report that the previously proposed object was indeed the progenitor star of SN 2005gl. This very massive star was likely a luminous blue variable that standard stellar evolution predicts should not have exploded in that state.

  2. plas.io: Open Source, Browser-based WebGL Point Cloud Visualization

    Science.gov (United States)

    Butler, H.; Finnegan, D. C.; Gadomski, P. J.; Verma, U. K.

    2014-12-01

    Point cloud data, in the form of Light Detection and Ranging (LiDAR), RADAR, or semi-global matching (SGM) image processing, are rapidly becoming a foundational data type to quantify and characterize geospatial processes. Visualization of these data, due to overall volume and irregular arrangement, is often difficult. Technological advancement in web browsers, in the form of WebGL and HTML5, have made interactivity and visualization capabilities ubiquitously available which once only existed in desktop software. plas.io is an open source JavaScript application that provides point cloud visualization, exploitation, and compression features in a web-browser platform, reducing the reliance for client-based desktop applications. The wide reach of WebGL and browser-based technologies mean plas.io's capabilities can be delivered to a diverse list of devices -- from phones and tablets to high-end workstations -- with very little custom software development. These properties make plas.io an ideal open platform for researchers and software developers to communicate visualizations of complex and rich point cloud data to devices to which everyone has easy access.

  3. Abscesso cervical gigante provocado por sialolitíase de glândula submandibular

    Directory of Open Access Journals (Sweden)

    Fernanda Fruet

    2016-10-01

    Full Text Available Introdução: As infecções cervicais profundas apresentam alta mortalidade, apesar da eficácia da antibioticoterapia atual. Uma das principais complicações é a obstrução das vias aéreas superiores. A principal causa dessas infecções é de origem odontogênica e, raramente, a infecção das glândulas salivares. Objetivo: Apresentar um caso de abscesso cervical profundo, cuja etiologia rara está associada à sialolitíase com origem em glândula submandibular. Metodologia: O caso foi acompanhado no serviço de Cabeça e Pescoço do Conjunto Hospitalar de Sorocaba (CHS e sua descrição embasada na literatura. Relato de Caso: Paciente iniciou quadro de dor localizada em região submandibular posterior direita, acompanhada de disfagia e dispneia contínua. Procurou atendimento médico na cidade de origem, onde recebeu antibioticoterapia. Evoluiu com insuficiência respiratória, necessitando de transferência para o serviço de emergência do CHS aos cuidados da equipe de Cabeça e Pescoço. Ao exame físico, apresentava edema, abaulamento e hiperemia da lateral direita do pescoço. A Tomografia Computadorizada evidenciou abscesso em espaço retro-faríngeo com compressão da parede posterior da orofaringe e hipofaringe, além de glândula submandibular direita aumentada e imagem de cálculo. Foi realizada a traqueostomia e a resolução cirúrgica por meio da drenagem do abscesso através da incisão transversa em colar. Conclusão: O caso apresentado mostra um abscesso cervical profundo causado por etiologia incomum, a sialolitíase, cujo diagnóstico precoce, seguido do tratamento adequado, poderiam ter evitado a evolução grave do paciente. Neste caso, a drenagem cirúrgica precoce mostra sua importância na prevenção de insuficiência respiratória.

  4. Morfologia da glândula mamária do quati (Nasua nasua

    Directory of Open Access Journals (Sweden)

    Juliana B. Casals

    2013-11-01

    Full Text Available Nasua nasua é um animal onívoro, encontrado em todo Brasil. A reprodução da espécie ocorre somente uma vez ao ano, na primavera. As fêmeas são matriarcas e amamentam suas crias até os 5 meses de idade, vivem com seus filhotes em bandos de até 30 indivíduos. Para descrição morfológica da glândula mamaria do Nasua nasua foram utilizados seis animais provenientes do Criatório Cientifico (Cecrimpas, Unifeob. Autorizado pelo Ibama (Proc.02027.002322/98-99. Para análise macroscópica um animal foi injetado com látex neoprene, sendo a artéria femoral injetada com látex de cor vermelha e a veia jugular de cor azul. Os demais animais foram fixados em solução aquosa a 10% de formaldeído. Para análise microscópica, fragmentos glandulares foram coletados e submetidos ao processo rotineiro, embebido em parafina e corados com Hematoxilina e Eosina, Picrossírius e Azul de Toluidina. Macroscopicamente foram evidenciados três pares de glândulas mamárias, sendo dois pares posicionados na região abdominal e um par na região inguinal. Microscopicamente, notou-se epitélio de revestimento externo das papilas mamárias, epitélio pavimentoso estratificado queratinizado, o qual seguia por toda glândula de forma irregular. Na entrada do óstio, o epitélio da epiderme era modificado ocorrendo uma transição de epitélio pavimentoso estratificado para um epitélio cúbico no ducto papilar. O parênquima glandular era caracteristicamente túbulo alveolar com células secretoras, evidenciado principalmente no animal lactente. Os resultados macroscópicos e microscópicos assemelham-se aos já descritos nas cadelas (Canis familiaris e os do Procyon cancrivorus pertencente à mesma família do quati, Família Procyonidae.

  5. Influência do consumo de etanol nas glândulas salivares

    Directory of Open Access Journals (Sweden)

    Carrard, Vinicius Coelho et al.

    2007-01-01

    Full Text Available Objetivos: Realizar uma revisão da literatura sobre os efeitos do consumo de etanol nas glândulas salivares. Fonte de dados: Foi realizada uma busca no banco de dados Medline, utilizando as palavras alcohol/ ethanol, salivary glands. Síntese dos dados: Tem sido observada na população uma elevação no consumo de bebidas alcoólicas pela população, ocasionando alterações em diversos sistemas. Aumento de volume das glândulas salivares e redução do fluxo salivar são freqüentes em indivíduos alcoolistas. Como a saliva tem propriedades protetoras, a redução do fluxo torna a boca mais suscetível a injúrias, como as relacionadas à cárie e à periodontite. Podem ser observadas alterações morfológicas, como acúmulo de tecido adiposo, hiperplasia dos ductos e redução da proporção de células acinares. O consumo de etanol é responsável ainda pela redução dos níveis de imunoglobulinas presentes na saliva. A degradação do etanol gera metabólitos tóxicos, como o acetaldeído, que podem modificar a função das glândulas salivares. Existe a possibilidade de a saliva redistribuir o acetaldeído na boca, potencializando o dano produzido pelo consumo de etanol. Além disso, outros mecanismos podem estar presentes, como redução das prostaglandinas, desnutrição, alterações do sistema imune e estresse oxidativo, este último envolvido na patogênese de uma série de doenças crônico-degenerativas, entre elas o câncer. Conclusões: Os pacientes alcoolistas devem receber uma atenção especial em relação aos cuidados bucais, pois suas condições locais e sistêmicas os tornam mais suscetíveis a doenças da boca, como cárie, periodontite e câncer.

  6. GPU acceleration for digitally reconstructed radiographs using bindless texture objects and CUDA/OpenGL interoperability.

    Science.gov (United States)

    Abdellah, Marwan; Eldeib, Ayman; Owis, Mohamed I

    2015-01-01

    This paper features an advanced implementation of the X-ray rendering algorithm that harnesses the giant computing power of the current commodity graphics processors to accelerate the generation of high resolution digitally reconstructed radiographs (DRRs). The presented pipeline exploits the latest features of NVIDIA Graphics Processing Unit (GPU) architectures, mainly bindless texture objects and dynamic parallelism. The rendering throughput is substantially improved by exploiting the interoperability mechanisms between CUDA and OpenGL. The benchmarks of our optimized rendering pipeline reflect its capability of generating DRRs with resolutions of 2048(2) and 4096(2) at interactive and semi interactive frame-rates using an NVIDIA GeForce 970 GTX device.

  7. Tratamiento quirúrgico de la glándula parótida

    OpenAIRE

    Alonso Castañeira, Ignacio; Escapa Garrachón, José María; Alonso Treceño, José Luis

    2015-01-01

    [ES]Introducción y objetivo: La cirugía de la glándula parótida es de especial interés entre las cirugías de cabeza y cuello debido a la desproporción entre el alto porcentaje de patología benigna y las graves consecuencias estéticas que se pueden derivar de una mala ejecución de la técnica, la prolongación del tiempo quirúrgico y la excesiva manipulación de las fibras nerviosas. En contraposición a esta alerta, el conocimiento anatómico de la celda parotídea así como de la base de cráneo nos...

  8. Non-diagonal boundary conditions for gl(1|1) super spin chains

    Energy Technology Data Exchange (ETDEWEB)

    Grabinski, Andre M; Frahm, Holger, E-mail: frahm@itp.uni-hannover.d [Institut fuer Theoretische Physik, Leibniz Universitaet Hannover, Appelstrasse 2, 30167 Hannover (Germany)

    2010-01-29

    We study a one-dimensional model of free fermions with gl(1|1) supersymmetry and demonstrate how non-diagonal boundary conditions can be incorporated into the framework of the graded quantum inverse scattering method (gQISM) by means of super matrices with entries from a superalgebra. For super Hermitian twists and open boundary conditions subject to a certain constraint, we solve the eigenvalue problem for the super transfermatrix by means of the graded algebraic Bethe ansatz technique (gABA) starting from a fermionic coherent state. For generic boundary conditions the algebraic Bethe ansatz cannot be applied. In this case the spectrum of the super transfermatrix is obtained from a functional relation.

  9. Tuberculosis primaria de la glándula suprarrenal: comunicación de dos casos

    Directory of Open Access Journals (Sweden)

    Natalia Coras

    2013-07-01

    Full Text Available En muchas áreas del mundo, la tuberculosis tiene gran prevalencia y sigue siendo la principal causa de insuficiencia suprarrenal. Clínicamente, esta enfermedad permanece sin sintomatología durante sus primeros estadios, debido a que la pérdida de la función adrenal suele ser progresiva; los síntomas tienen aparición gradual e insidiosa, en su mayoría inespecíficos. Presentamos el caso de dos mujeres con cuadro clínico de insuficiencia adrenal primaria crónica, con afección bilateral de ambas glándulas suprarrenales y resultado histopatológico de infección activa por tuberculosis.

  10. A browser-based event display for the CMS Experiment at the LHC using WebGL

    Science.gov (United States)

    McCauley, T.

    2017-10-01

    Modern web browsers are powerful and sophisticated applications that support an ever-wider range of uses. One such use is rendering high-quality, GPU-accelerated, interactive 2D and 3D graphics in an HTML canvas. This can be done via WebGL, a JavaScript API based on OpenGL ES. Applications delivered via the browser have several distinct benefits for the developer and user. For example, they can be implemented using well-known and well-developed technologies, while distribution and use via a browser allows for rapid prototyping and deployment and ease of installation. In addition, delivery of applications via the browser allows for easy use on mobile, touch-enabled devices such as phones and tablets. iSpy WebGL is an application for visualization of events detected and reconstructed by the CMS Experiment at the Large Hadron Collider at CERN. The first event display developed for an LHC experiment to use WebGL, iSpy WebGL is a client-side application written in JavaScript, HTML, and CSS and uses the WebGL API three.js. iSpy WebGL is used for monitoring of CMS detector performance, for production of images and animations of CMS collisions events for the public, as a virtual reality application using Google Cardboard, and asa tool available for public education and outreach such as in the CERN Open Data Portal and the CMS masterclasses. We describe here its design, development, and usage as well as future plans.

  11. Glándula pediosa de moluscos terrestres y sus implicancias evolutivas, con énfasis en Megalobulimus

    Directory of Open Access Journals (Sweden)

    Victor Borda

    2011-05-01

    Full Text Available Se describe la anatomía de la glándula pediosa de cinco especies de Megalobulimus (Megalobulimidae, y son comparadas con gastrópodos pulmonados Succineidae, Orthalicidae, Helicidae y Veronicellidae. Una sinapomorfía considerada para el clado Stylommatophora es la presencia de una membrana que aísla a la glándula pediosa de la cavidad visceral. Las especies estudiadas mostraron un grado variable de sujeción de la glándula al pie, desde apenas sujeta por escasa fibras (Megalobulimus hasta aquella totalmente aislada por una membrana (Cantareus pasando por grados intermedios (Succinea y Bostryx. La glándula pediosa en la seudobabosa Heterovaginia limayana (Systellommatophora no está adherida al piso de la cavidad visceral. En Megalobulimus, la parte glandular es voluminosa y pende del techo de la cápsula, a diferencia de las otras especies en que los acinos glandulares están uniformemente adosados a la pared interna de la cápsula. Se describen nuevos caracteres para la glándula pediosa que mejoran la diagnosis del género Megalobulimus y su evaluación filogenética.

  12. A dioxin response element in the multiple cloning site of the pGL3 luciferase reporter influences transcriptional activity.

    Science.gov (United States)

    Ochs, Sharon D; Liu, Jing; Fernando, Tharu M; Fecher, Roger A; Sulentic, Courtney E W

    2012-09-01

    Luciferase reporter plasmids (pGL3 backbone, Promega) have been utilized to characterize the transcriptional effects of the environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and other aryl hydrocarbon receptor (AhR) ligands. Following ligand activation, the AhR and its dimerization partner AhR nuclear translocator (ARNT) regulate transcription by binding dioxin response elements (DREs) in regulatory regions of dioxin-sensitive genes. Upon sequencing of our luciferase reporters, we unexpectedly identified a DRE core motif within the multiple cloning site (mcsDRE) of the pGL3 luciferase plasmid backbone in a subset of our reporters. Therefore, the objective of this study was to determine if the mcsDRE inadvertently influences reporter activity. Utilizing deletional analysis we determined that the mcsDRE did significantly alter the transcriptional effect induced by TCDD. Since many chemicals have been shown to interact with the AhR and influence transcription through the DRE, the presence of the mcsDRE in the pGL3 luciferase plasmid may inappropriately influence promoter and enhancer analysis. As such, insertion of regulatory elements into pGL3 reporters should be designed to avoid retaining the mcsDRE core motif (GCGTG) and currently utilized pGL3 reporters should be evaluated for the presence of the mcsDRE. Copyright © 2012 Elsevier Ltd. All rights reserved.

  13. Direcionadores de preferência e perfil sensorial de pães isentos de glúten e sacarose

    OpenAIRE

    Natália Manzatti Machado Alencar

    2014-01-01

    Resumo: Na atualidade a doença celíaca atinge aproximadamente 1% da população do mundo, e cerca de 3,1% dos pacientes com diabetes mellitus tipo I. A doença celíaca é caracterizada pela atrofia da mucosa intestinal na parte proximal e pela permanente intolerância ao glúten. Seu tratamento consiste em uma dieta isenta de glúten. Novas estratégias tem sido utilizadas para a fabricação de produtos de panificação isentos de glúten e sacarose, que se referem à utilização de pseudocereais, tais com...

  14. The Murid Herpesvirus-4 gL regulates an entry-associated conformation change in gH.

    Directory of Open Access Journals (Sweden)

    Laurent Gillet

    2008-07-01

    Full Text Available The glycoprotein H (gH/gL heterodimer is crucial for herpesvirus membrane fusion. Yet how it functions is not well understood. The Murid Herpesvirus-4 gH, like that of other herpesviruses, adopts its normal virion conformation by associating with gL. However, gH switched back to a gL-independent conformation after virion endocytosis. This switch coincided with a conformation switch in gB and with capsid release. Virions lacking gL constitutively expressed the down-stream form of gH, prematurely switched gB to its down-stream form, and showed premature capsid release with poor infectivity. These data argue that gL plays a key role in regulating a gH and gB functional switch from cell binding to membrane fusion.

  15. Estereologia das glândulas bulbouretrais do coelho (Oryctolagus cuniculus e da cobaia (Cavia porcellus

    Directory of Open Access Journals (Sweden)

    Bélgica Vásquez

    2014-12-01

    Full Text Available As glândulas bulbouretrais (GBU no coelho (Oryctolagus cuniculus e na cobaia (Cavia porcellus desempenham um papel importante na fisiologia reprodutiva. No entanto, seus aspectos histológico e estereológico são escassos. Assim, o objetivo desta pesquisa foi comparar características estereológicas entre as GBU do coelho e da cobaia como um primeiro passo para a compreensão das variáveis morfométricas que participam nos processos reprodutivos. Foram utilizados 5 coelhos e 5 cobaias adultos machos, saudáveis, obtidos do Biotério da Universidade de La Frontera, Temuco, Chile. A região pélvica foi dissecada isolando-se a GBU de cada animal. Determinou-se o peso e o volume de cada glândula. Estas foram fixadas em formalina tamponada durante 24 horas e incluídas em paraplast. Cortes seriados de 4 μm de espessura foram corados com HE para análise estereológica. A média de células glandulares na GBU do coelho foi 19,50 x 10(5mm³ (DP 2,35 e da cobaia 10,57 x 10(5mm³ (DP 2,07 e a porcentagem média de tecido glandular foi de 25,52% (DP 2,20 e 17,20% (DP 3,33, respectivamente. Todos os parâmetros estereológicos comparados tiveram uma diferença estatisticamente significativa (p<0,0001. Estas diferenças poderiam ser explicadas porque há maior proximidade celular do epitélio secretor, menor diâmetro do lúmen dos ácinos e da relação núcleo citoplasma na GBU do coelho. Assim, os ácinos da GBU apresentam maior quantidade de células por mm³ do que na GBU do coelho. Estes parâmetros podem ser influenciados por fatores hormonais, etários, sazonais e ambientais, entre outros. Considerar as características morfológicas da GBU nesses animais poderia condicionar o êxito da reprodução por parte do macho.

  16. Transplante de glândulas salivares menores no tratamento da ceratoconjuntivite seca em cães

    Directory of Open Access Journals (Sweden)

    G.T Angélico

    2011-10-01

    Full Text Available Relatou-se o efeito do transplante de glândulas salivares menores (TGSM em cães portadores de ceratoconjuntivite seca (CCS e estudaram-se os efeitos da secreção dessas glândulas usadas como alternativa de lubrificação ocular. A aplicação da técnica foi satisfatória, uma vez que resultou em melhora no quadro clínico oftalmológico sem que houvesse mínimas intercorrências pós-operatórias.

  17. Simulator Posisi Matahari dan Bulan Berbasis Web dengan WebGL

    Directory of Open Access Journals (Sweden)

    Kamshory

    2014-09-01

    Full Text Available Moon as a satellite of the earth has an important role for the life of the earth. Apart from being a source of illumination, moon also has an effect on the earth both on land and at sea. The influence of the moon to the earth relate to the its position. This research aims to create a simulator of the sun and moon position to the earth based on the time and location of the observation. This simulator is web-based and made in 3-dimensional (3D using webGL technology. The position of the sun and moon expressed by latitude and longitude which is a position that is passed by the line connecting the earth to the sun or the line connecting the earth to the moon. The position of the sun and moon obtained from calculations based on previous research. Once the position is known, the earth, the sun, and the moon then described as a 3-D model. The position of the camera can be moved by dragging a web page so that the sun, earth, and moon can be seen from various positions. The camera always leads to the center of the earth to avoid user errors flipping the camera which causes the object is not visible.

  18. Condroma de la glándula parótida

    Directory of Open Access Journals (Sweden)

    Yorgel Reyes Álvarez

    2012-12-01

    Full Text Available El condroma es una neoplasia benigna, de origen mesenquimatoso, constituida por tejido cartilaginoso, que en el área maxilofacial es rara. Su sitio más frecuente en el complejo craneofacial es la estructura ósea maxilar o mandibular, aunque también se han descrito condromas de los tejidos blandos. Clínicamente se presenta como una lesión de crecimiento lento, asintomático, no dolorosa, de consistencia duro elástica, con una gradual expansión. El tratamiento de elección es la resección quirúrgica. Se tuvo como objetivo publicar esta infrecuente ubicación de la neoplasia por la dificultad que se presenta al diagnóstico diferencial. Presentamos un caso clínico de un tumor localizado en el lóbulo profundo de la glándula parótida, cuyo diagnóstico inicial, tras la citología por aspiración con aguja fina, fue de adenoma pleomorfo, lo que se comprobó luego por examen histopatológico de la pieza quirúrgica que se trataba de un condroma. Se concluye que ante una tumoración de parótida, siempre debe tenerse en cuenta el condroma en el diagnóstico diferencial.

  19. Cycloplegic effects of 0.5g/L tropicamide in various low myopia age groups

    Directory of Open Access Journals (Sweden)

    Hai-Rong Zhang

    2014-12-01

    Full Text Available AIM: To investigated the refraction and cycloplegic effects of 0.5g/L tropicamide eye drops in various low myopia age groups, and provide clinical guidelines for proper usage of cyclopegic agent in different population. METHODS: Three low myopia age groups(n=45were selected, non-cycloplegic and cycloplegic auto-refraction using “Shin-Nippon” auto-refractor were performed at various time point for refractive error. The subjective accommodation amplitudes were also collected at each time point.RESULTS: The accommodation amplitude showed a similar but significant decline between 20~60min after the first cyclopegic drops in all three age groups. However, the decline of accommodation amplitudes were not statistically correlated with the results of “Shin-Nippon”auto-refraction results at different time points. CONCLUSION: The refraction time should be arranged according to the cycloplegic effects of tropicamide. Open-field auto-refractor could reduce the usage of cycloglegice drugs in low myopia groups, even for the youngest.

  20. Highly abundant HCN in the inner hot envelope of GL 2591 : probing the birth of a hot core?

    NARCIS (Netherlands)

    Boonman, A. M. S.; Stark, R.; Tak, F. F. S. van der; Dishoeck, E. F.; Schafer, F.; Lange, G de; Laauwen, W. M.; van der Wal, P.B.

    2004-01-01

    Published in: Astrophys. J. 553 (2001) L63-L68 citations recorded in [Science Citation Index] Abstract: We present observations of the v2=0 and vibrationally excited v2=1 J=9-8 rotational lines of HCN at 797 GHz toward the deeply embedded massive young stellar object GL 2591, which provide the

  1. Changes in keratin 8/18 expression in human granulosa cell lineage are associated to cell death/survival events: potential implications for the maintenance of the ovarian reserve.

    Science.gov (United States)

    Gaytan, F; Morales, C; Roa, J; Tena-Sempere, M

    2018-02-01

    Is keratin 8/18 (K8/K18) expression linked to cell death/survival events in the human granulosa cell lineage? A close association exists between changes in K8/K18 expression and cell death/survival events along the human granulosa cell lineage lifespan. In addition to their structural and mechanical functions, K8/K18 play essential roles regulating cell death, survival and differentiation in several non-gonadal epithelial tissues. Transfection of the granulosa-like tumor KGN cells with siRNA to interfere KRT8 and KRT18 expression increases FAS-mediated apoptosis, while an inverse association between K8/K18 expression and cell death has been found in the bovine antral follicles and corpus luteum. Yet, only fragmentary and inconclusive information exists regarding K8/K18 expression in the human ovary. Expression of K8/K18 was assessed by immunohistochemistry at different stages of the granulosa cell lineage, from flattened granulosa cells in primordial follicles to fully luteinized granulosa-lutein cells in the corpus luteum (including corpus luteum of pregnancy). Immunohistochemical detection of K8/K18 was conducted in 40 archival ovarian samples from women aged 17-39 years. K8/K18 expression was analyzed at the different stages of follicle development and corpus luteum lifespan. The proportions of primordial follicles showing all K8/K18-positive, all K8/K18 negative, or a mixture of K8/K18 negative and positive granulosa cells were quantified in 18 ovaries, divided into three age groups: ≤ 25 years (N = 6), 26-30 (N = 6) and 31-36 (N = 6) years. A total number of 1793 primordial, 750 transitional and 140 primary follicles were scored. A close association was found between changes in K8/K18 expression and cell death/cell survival events in the human granulosa cell lineage. Large secondary and early antral follicles (most of them undergoing atresia) and regressing corpora lutea displayed low/absent K8/K18 expression. Conversely, early growing and some large antral

  2. Classificação Morfológica da Glândula Prepucial do Nasua nasua (Quati.

    Directory of Open Access Journals (Sweden)

    Bruno Machado Bertassoli

    2011-05-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2011v24n2p83 A proposta desta pesquisa é descrever a glândula prepucial do Nasua nasua. Utilizaram-se oito quatis provenientes do Criatόrio Científico Cecrimpas (Unifeob; as glândulas prepuciais foram analisadas, dissecadas e descritas macro e microscopicamente, visando estabelecer sua classificação morfofuncional para, assim, poder compará-las com as de outras espécies já descritas na literatura. Amostras de tecido glandular foram coletadas, coradas (Hematoxilina e Eosina, Azul Toluidina, e Picrosirius e analisadas através da microscopia de luz. Todos os resultados foram compilados e fotodocumentados. Na espécie citada, essa glândula tem como principal função a demarcação territorial e está situada na porção prepucial do mesmo modo que nos javalis. Em outros animais, como primatas, cervídeos e roedores, essas glândulas demarcatόrias estão localizadas em diferentes regiões anatômicas, como entre os olhos, no metatarso, nas áreas perianais e esternais. Em nosso resultado das medidas biométricas, obtivemos como média 3,8 ± 1,41cm de largura, 3,15 ± 0,93cm de altura e 10,26 ± 1,89cm de circunferência, e essa glândula foi histologicamente classificada como do tipo tubuloalveolar, com secreção holόcrina.

  3. Transfusiones de glóbulos rojos en pacientes críticos

    Directory of Open Access Journals (Sweden)

    Carolina Ruiz Balart

    2017-11-01

    Full Text Available La anemia es muy frecuente en los pacientes críticos,  pudiendo estar presente en casi el 100% después una semana de hospitalización enla Unidadde Cuidados Intensivos (UCI. Se ha descrito que la anemia es un factor de riesgo de mayor mortalidad y morbilidad para los pacientes críticos. La etiología de la anemia en UCI es multifactorial, destacando la anemia de la inflamación, los déficits nutricionales, la hemodilución y el aumento de las pérdidas. Dentro del aumento de las pérdidas destaca la toma seriada de exámenes de sangre. A pesar de lo deletéreo de la anemia, no se ha demostrado que las transfusiones de glóbulos rojos (GR puedan mejorar el pronóstico de los pacientes con anemia. Por el contrario, hay estudios que han descrito un aumento de las complicaciones y mortalidad asociadas al aporte de GR. La evidencia actual sugiere que en pacientes críticos estables y que no presentan sangrado activo, las transfusiones deberían evitarse si la concentración de hemoglobina es mayor a 7 g/dL. Sin embargo, un umbral transfusional estricto puede no ser adecuado para todos los pacientes, por lo que también deberían considerarse las alteraciones de la perfusión y la condición cardíaca y respiratoria en la decisión de transfundir GR.

  4. The Endoplasmic Reticulum-Associated Maize GL8 Protein Is a Component of the Acyl-Coenzyme A Elongase Involved in the Production of Cuticular Waxes1

    Science.gov (United States)

    Xu, Xiaojie; Dietrich, Charles R.; Lessire, Rene; Nikolau, Basil J.; Schnable, Patrick S.

    2002-01-01

    The gl8 gene is required for the normal accumulation of cuticular waxes on maize (Zea mays) seedling leaves. The predicted GL8 protein exhibits significant sequence similarity to a class of enzymes that catalyze the reduction of a ketone group to a hydroxyl group. Polyclonal antibodies raised against the recombinant Escherichia coli-expressed GL8 protein were used to investigate the function of this protein in planta. Subcellular fractionation experiments indicate that the GL8 protein is associated with the endoplasmic reticulum membranes. Furthermore, polyclonal antibodies raised against the partially purified leek (Allium porrum) microsomal acyl-coenzyme A (CoA) elongase can react with the E. coli-expressed GL8 protein. In addition, anti-GL8 immunoglobulin G inhibited the in vitro elongation of stearoyl-CoA by leek and maize microsomal acyl-CoA elongase. In combination, these findings indicate that the GL8 protein is a component of the acyl-CoA elongase. In addition, the finding that anti-GL8 immunoglobulin G did not significantly inhibit the 3-ketoacyl-CoA synthase, 3-ketoacyl-CoA dehydrase, and (E) 2,3-enoyl-CoA reductase partial reactions of leek or maize acyl-CoA elongase lends further support to our previous hypothesis that the GL8 protein functions as a β-ketoacyl reductase during the elongation of very long-chain fatty acids required for the production of cuticular waxes. PMID:11891248

  5. Regionalización histológica de la glándula dorsal del pecarí de collar (Artiodactyla, Tayassuidae: Pecari tajacu)

    OpenAIRE

    Juan Carlos González Morales; Víctor Fajardo; Arturo Luna Blasio; Palestina Guevara-Fiore; Jorge Rodríguez-Antolín; Erendira Quintana

    2015-01-01

    Se describe histológicamente la glándula dorsal de machos y hembras del pecarí de collar en tres regiones: craneal, media y caudal. Con las tinciones Hematocilinaeosina y Tricrómica de Masson observamos una clara regionalización morfológica e histológica de esta glándula y se describe por primera vez la presencia de glándulas tubuloacinares en la región craneal de la glándula dorsal del macho de pecarí, cuya función es aún desconocida.

  6. Regionalización histológica de la glándula dorsal del pecarí de collar (Artiodactyla, Tayassuidae: Pecari tajacu

    Directory of Open Access Journals (Sweden)

    Juan Carlos González Morales

    2015-01-01

    Full Text Available Se describe histológicamente la glándula dorsal de machos y hembras del pecarí de collar en tres regiones: craneal, media y caudal. Con las tinciones Hematocilinaeosina y Tricrómica de Masson observamos una clara regionalización morfológica e histológica de esta glándula y se describe por primera vez la presencia de glándulas tubuloacinares en la región craneal de la glándula dorsal del macho de pecarí, cuya función es aún desconocida.

  7. Condroma de la glándula parótida Parotid gland chondroma

    Directory of Open Access Journals (Sweden)

    Yorgel Reyes Álvarez

    2012-12-01

    Full Text Available El condroma es una neoplasia benigna, de origen mesenquimatoso, constituida por tejido cartilaginoso, que en el área maxilofacial es rara. Su sitio más frecuente en el complejo craneofacial es la estructura ósea maxilar o mandibular, aunque también se han descrito condromas de los tejidos blandos. Clínicamente se presenta como una lesión de crecimiento lento, asintomático, no dolorosa, de consistencia duro elástica, con una gradual expansión. El tratamiento de elección es la resección quirúrgica. Se tuvo como objetivo publicar esta infrecuente ubicación de la neoplasia por la dificultad que se presenta al diagnóstico diferencial. Presentamos un caso clínico de un tumor localizado en el lóbulo profundo de la glándula parótida, cuyo diagnóstico inicial, tras la citología por aspiración con aguja fina, fue de adenoma pleomorfo, lo que se comprobó luego por examen histopatológico de la pieza quirúrgica que se trataba de un condroma. Se concluye que ante una tumoración de parótida, siempre debe tenerse en cuenta el condroma en el diagnóstico diferencial.The chondroma is a benign tumor of mesenchymal origin, consisted of a cartilaginous tissue, and occurs rarely in the maxillofacial area. Its most frequent site in the craniofacial complex is the maxillary bony structure, although chondromas of the soft tissues have also been described in the literature. From the clinical viewpoint, it appears as a painless, hard elastic lesion of slow asymptomatic growth, with gradual expansion. The treatment of choice is surgery. This paper presented a clinical case of a tumor located in the deep lobe of the parotid gland. The initial diagnosis, after the fine needle aspiration test, was pleomorphic adenoma, which was then subjected to histopathologic testing to obtain the final diagnosis of chondroma. Concluding that although uncommon in this localization, it should always be kept in mind as possible diagnosis. It was concluded that when

  8. Carcinoma da glândula supra-renal Adrenal gland carcinoma

    Directory of Open Access Journals (Sweden)

    Alexandre Coutinho Teixeira de Freitas

    2007-09-01

    Full Text Available RACIONAL: A neoplasia da glândula supra-renal pode ser oriunda de diversos tipos histológicos. O carcinoma é raro, correspondendo à 0,02% de todas as neoplasias. OBJETIVOS: Revisar os últimos avanços relacionados ao diagnóstico e ao tratamento do carcinoma da glândula supra-renal. MÉTODOS:Foi realizada revisão da literatura no Medline, livros texto de autores consagrados e referências obtidas de artigos considerados de maior relevância. CONCLUSÕES: Aproximadamente 79% dos carcinomas supra-renais são funcionantes. O hormônio mais comumente secretado é o cortisol causando síndrome de Cushing. Portadores de lesões não-funcionantes podem apresentar queixas relacionadas com o crescimento local da lesão. Ele está presente em uma série de síndromes neoplásicas de origem familiar. Ainda não está definido se o carcinoma esporádico tem origem em lesões benignas. De acordo com os sintomas, a investigação diagnóstica inicial envolve dosagens de cortisol urinário e nível sérico de aldosterona e renina. A tomografia abdominal ou a ressonância magnética são os exames de imagem de primeira linha. O PET scan com fluorodeoxiglicose apresenta potencial para diferenciação entre lesão benigna e maligna. A biópsia com agulha fina não está indicada devido à sua alta taxa de complicações. O tratamento de eleição é o cirúrgico com ressecção em bloco de órgãos adjacentes se necessário. Linfadenectomia aórtica e retroperitoneal deve ser realizada. Recorrência local ou metástases ocorrem em 80% dos casos. Procedimentos cirúrgicos citoredutores beneficiam os casos de doença avançada. Na presença de metástases, nos submetidos à cirurgia citoredutora e nos com recorrência local à quimioterapia com mitotano está indicada. Radioterapia é o tratamento de escolha no caso de metástases ósseas e tratamento adjuvante em alguns casos de risco elevado para recorrência. Em adultos a sobrevida média geral em 5 anos

  9. Acoustic emission analysis of crack resistance and fracture behavior of 20GL steel having the gradient microstructure and strength

    Science.gov (United States)

    Nikulin, S.; Nikitin, A.; Belov, V.; Rozhnov, A.; Turilina, V.; Anikeenko, V.; Khatkevich, V.

    2017-07-01

    The crack resistances as well as fracture behavior of 20GL steel quenched with a fast-moving water stream and having gradient microstructure and strength are analyzed. Crack resistance tests with quenched and normalized flat rectangular specimens having different cut lengths loaded by three-point bending with acoustic emission measurements have been performed. The critical J-integral has been used as the crack resistance parameter of the material. Quenching with a fast moving water stream leads to gradient (along a specimen wall thickness) strengthening of steel due to highly refined gradient microstructure formation of the troostomartensite type. Quenching with a fast-moving water stream increases crack resistance Jc , of 20GL steel by a factor of ∼ 1.5. The fracture accrues gradually with the load in the normalized specimens while the initiated crack is hindered in the variable ductility layer and further arrested in the more ductile core in the quenched specimens.

  10. Design and implementation of virtual fault diagnosis system for photoelectric tracking devices based on OpenGL

    Science.gov (United States)

    Hou, MingLiang; Li, Cunhua; Zhang, Yong; Su, Liyun

    2009-10-01

    In view of the crucial deficiency of the traditional diagnosis approaches for photoelectric tracking devices and the output of more sufficient diagnosis information, in this paper, an virtual fault diagnosis system based on open graphic library(OpenGL) is proposed. Firstly, some interrelated key principles and technology of virtual reality, visualization and intelligent fault diagnosis technology are put forward. Then, the demand analysis and architecture of the system are elaborated. Next, details of interrelated essential implementation issues are also discussed, including the the 3D modeling of the related diagnosis equipments, key development process and design via OpenGL. Practical applications and experiments illuminate that the proposed approach is feasible and effective.

  11. Implementing WebGL and HTML5 in Macromolecular Visualization and Modern Computer-Aided Drug Design.

    Science.gov (United States)

    Yuan, Shuguang; Chan, H C Stephen; Hu, Zhenquan

    2017-06-01

    Web browsers have long been recognized as potential platforms for remote macromolecule visualization. However, the difficulty in transferring large-scale data to clients and the lack of native support for hardware-accelerated applications in the local browser undermine the feasibility of such utilities. With the introduction of WebGL and HTML5 technologies in recent years, it is now possible to exploit the power of a graphics-processing unit (GPU) from a browser without any third-party plugin. Many new tools have been developed for biological molecule visualization and modern drug discovery. In contrast to traditional offline tools, real-time computing, interactive data analysis, and cross-platform analyses feature WebGL- and HTML5-based tools, facilitating biological research in a more efficient and user-friendly way. Copyright © 2017 Elsevier Ltd. All rights reserved.

  12. Cálculos em glândulas salivares: uma revisão crítica da literatura

    OpenAIRE

    Ferreira, José Lucas Soares; Souto, Ingrid Carneiro Cavalcante; Universidade Federal de Campina Grande; Alves, Emanuelle Ferreira; Oliveira, Heloísa Mara Batista Fernandes de; Alves, Maria Angélica Satyro Gomes; Oliveira Filho, Abrahão Alves de

    2016-01-01

    O presente estudo objetiva realizar uma revisão critica da literatura sobre sialólitos do ponto de vista de desenvolvimento, etiologia, sintomatologia, diagnóstico e tratamento. Os sialólitos são calcificações que obstruem o interior das glândulas salivares ou o interior de seus ductos, compostos por um núcleo de substância amorfa envolto de íons provenientes da saliva. A sialolitíase é a doença mais comum das glândulas salivares, possui uma predileção por homens de meia idade. Os sialólitos ...

  13. Plasticidade de glândulas tegumentares abdominais em Monoeca xanthopyga Harter-Marques, Cunha & Moure (Hymenoptera, Apidae, Tapinotaspidini

    Directory of Open Access Journals (Sweden)

    Camila Gonçalves dos Santos

    2004-06-01

    Full Text Available Glândulas tegumentares são amplamente conhecidas em abelhas sociais e responsáveis por diversas funções nestes insetos. Entretanto, pouco se conhece sobre estas glândulas em abelhas solitárias. Monoeca xanthopyga é uma espécie de abelha solitária que exibe comportamento peculiar nas estratégias de acasalamento e no processo de nidificação. Este estudo visa verificar a ocorrência de glândulas tegumentares abdominais e a natureza química de produtos secretados por machos e fêmeas de M. xanthopyga, em dois momentos do ciclo vital: recém-emergidas e em período de nidificação. O material foi estudado utilizando-se microscopia óptica e eletrônica de varredura e histoquímica. Machos e fêmeas recém-emergidas e em período de nidificação apresentam glândulas tegumentares nos tergos III ao VII, entretanto diferem no tipo e na localização. Nos esternos de fêmeas recém-emergidas, as glândulas estão ausentes, já nas fêmeas em período de nidificação estas glândulas estão presentes nos esternos IV ao VI. Nos machos, as glândulas tegumentares estão presentes nos esternos IV ao VI. A análise histoquímica das glândulas tegumentares de machos e fêmeas sugere a presença de produtos de natureza lipídica, possivelmente envolvidos na comunicação relacionada ao comportamento sexual.Plasticity of abdominal tegumentar glands in Monoeca xanthopyga Harter-Marques, Cunha & Moure (Hymenoptera, Apidae, Tapinotaspidini. Tegumentar glands are highly known in social bees and are responsible for a diversity of functions in these insects. However, little is known about these glands in solitary bees. Monoeca xanthopyga is a solitary bee species that shows a singular behavior of mating and nesting. This study is to verify the occurrence of abdominal tegumentar glands and the chemical nature of the products secreted by males and females of M. xanthopyga, at two moments of the vital cycle: just emerged and at the nesting period. The

  14. Vascularização das glândulas adrenais em galinhas da linhagem NPK (Gallus gallus domesticus

    Directory of Open Access Journals (Sweden)

    Vanessa Coutinho do Amaral

    2004-11-01

    Full Text Available Com o objetivo de contribuir com o conhecimento referente aos arranjos vasculares viscerais, que freqüentemente mostram suficientes variações para diferenciação entre famílias e ordens, utilizamos 34 galinhas da linhagem NPK, com idade aproximada de 10 semanas, eutanasiadas com dose de gás anestésico, tendo marcado seus contingentes arteriais com solução aquosa de Neoprene Látex ¨450¨ a 50 %, corada com corante específico e fixadas em solução aquosa de formol a 10 %, para estudar a vascularização das glândulas adrenais. Assim, os resultados indicam que as glândulas adrenais são órgãos pares, dispostos um em cada antímero, lateralmente à aorta descendente, caudalmente aos pulmões e médio-cranialmente aos rins. Nos dois antímeros, as glândulas adrenais receberam vasos oriundos das artérias adrenais homólogas provenientes da artéria renal cranial e da aorta descendente (ramos diretos. Independentemente da origem, o número de ramos destinados às glândulas adrenais variou de acordo com o antímero, sendo de 1 a 4 para o antímero esquerdo, e de 1 e 3 para o direito. Quanto maior o número de artérias totais e ramos emitidos pelas artérias adrenais contralaterais destinados a um antímero, maior será o número de ramos destinados pelas mesmas ao antímero oposto.

  15. Linfoma tipo MALT de la glándula parótida Lymphoma type MALT of the parotid gland

    Directory of Open Access Journals (Sweden)

    Carlos Frómeta Neira

    2010-09-01

    Full Text Available Los linfomas tipo MALT (tejido linfoideo asociado a mucosa, constituyen la variedad más recientemente descubierta de los linfomas no Hodgkin, tienen lugar fundamentalmente en la mucosa gástrica asociados a infección por Helycobacter pylori, y en la glándula tiroides en relación con la tiroiditis de Hashimoto. Sin embargo, internacionalmente se han descrito casos en glándulas salivales asociados a linfoadenitis. La naturaleza de la lesión a menudo no puede ser determinada solo por el estudio citológico; se hace necesario el análisis histopatológico para el diagnóstico definitivo en la mayoría de los casos. El presente estudio muestra un paciente masculino con aumento de volumen bilateral de ambas glándulas parótidas y diagnóstico citohistopatológico de un proceso linfoepitelial benigno, el cual desarrolló un linfoma tipo MALT en relación con la glándula parótida derecha.The lymphomas type MALT or the mucosa-associated lymphoid tissue, are the most recent variety of non-Hodgkin lymphomas present mainly in the gastric mucosa associated with Helycobacter pylori infection and in the thyroid gland in relation to Hashimoto's thyroiditis. Frequently the origin of this lesion can't be determined only by cytology study, thus it is necessary the histopathology analysis for a definitive diagnosis in most cases. Present paper includes the case of male patient with bilateral volume increase of both parotid glands and a diagnosis cytopathological of a benign lymphoepithelial process and the development of a type MALT lymphoma in relation to the right parotid gland.

  16. Clinical effect of 1g/L fluorometholone drops combined with soft corneal contact lens for filamentary keratitis

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    Zhi-Bin Shen

    2015-09-01

    Full Text Available AIM: To study the clinical effect of 1g/L fluorometholone drops combined with soft corneal contact lens in the treatment of patients with filamentary keratitis. METHODS: A total of 30 patients(45 eyeswere included in this study and were randomly divided into two groups: 16 patients(23 eyeswere in treatment group and 14 patients(22 eyesin control group. In the treatment group, after removed filaments, the patients wore soft corneal contact lens and received corticosteroid eye drops(1g/L fluorometholone dropsand artificial tears(sodium hyaluronate. In the control group, patients received only artificial tears(sodium hyaluronateafter removed filaments. Clinical effects of the two groups were record. The patients were followed up for 3mo.RESULTS: The total clinical effective rate of the patients in the treatment group was significantly higher than that in the control group(22 eyes vs 17 eyes, 96% vs 77%. The difference between two groups had statistical significance(U=1.992, P=0.046. CONCLUSION: The results suggest that 1g/L fluorometholone drops combined with soft corneal contact lens is effective to patients with filamentary keratitis.

  17. Sôbre a glândula de oviduto ímpar de Odozona obscura Schs. (Arctiidae, Lithosiinae

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    Rudolf Barth

    1960-09-01

    Full Text Available A glândula da fêmea de Odozona obscura está situada na parte terminal do oviduto ímpar (homólogo à vagina. Sua origem é ectodérmica. è composta por um epitélio simples, formando uma dobra dupla, que substitui parcialmente a luz do duto. São apresentadas várias fases da função celular. A célula possui uma zona basal ergastoplasmática e, na parte apical, um rabdório muito alto. As secreções atravessam a cutícula de modo criptócrino, isto é, em gotículas menores que 0,1 µ. A função da glândula é desconhecida, podendo ser uma glândula atraente, ou colágena, produzindo uma substância para colar os ovos sôbre o substrato no momento da postura.

  18. Analysis of the Lunar Gravity Field by Using GL0660B Model and Its Effect on Lunar Satellite Orbit

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    HUANG Kunxue

    2016-07-01

    Full Text Available The lunar gravity field provides a way to research moon's evolution and probes the interior structure of the moon. It is an important factor influencing the lunar satellite precise orbit determination as well. The new lunar gravity model GL0660B from GRAIL mission dramatically improves the gravity spectrum and spectral ranges. Using the model GL0660B, it can be computed that the corresponding degree-wise RMS and correlation of topography, with which the quality of model GL0660B can be analyzed. Then different characters of the lunar gravity field comparing with other lunar gravity fields are analyzed. Besides, gravity anomaly distribution figures at different height of the models are given, and the character and difference of the lunar gravity models at different height are compared. In addition, lunar satellite orbit revolutionary at different height are modeled by GEODYN. The result shows that the trend of lunar satellite eccentricity changes is a complex and long cycle of change trend. It is different affected by the perturbation of the mascons of different height, which causes different changes of apolune, perilune and eccentricity.

  19. Detección y localización inmunohistoquímica del receptor de leptina en la glándula paratiroides de ratas

    OpenAIRE

    Vidal Carrascosa, Ángela

    2018-01-01

    Premio extraordinario de Trabajo Fin de Máster curso 2015-2016. Medicina, Sanidad y Mejora Animal La glándula paratiroides es una glándula endocrina involucrada en la homeostasis del calcio mediante la producción de la parathormona (PTH). La relación existente entre los niveles de leptina y PTH en obesidad e hiperparatiroidismo sugiere la presencia de receptores de leptina en la glándula paratiroides. La leptina es una adipoquina cuya función principal es la regulación del metabolismo ener...

  20. Cistomatose de glândula apócrina em onça-pintada (Panthera onca

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    Leonardo Gruchouskei

    Full Text Available RESUMO: A cistomatose de glândula apócrina é uma condição rara e não-neoplásica, que tem sido relatada em cães e gatos, e caracterizada por vários grupos de glândulas sudoríparas dilatadas, cuja distribuição generalizada raramente é observada. Uma fêmea de onça-pintada melânica (Panthera onca, com idade estimada de 16 anos e mantida em cativeiro, apresentou aumento de volume abdominal. Ao exame físico foram observados vários nódulos de consistência macia e flutuante, não aderidos, com tamanho de 2 a 15cm de diâmetro, na região ventral abdominal. Outras massas de menor diâmetro (0,5-1,0cm estavam presentes na região cervical e no dorso do animal. Estas massas foram removidas em um procedimento cirúrgico. Dentro deste cisto havia conteúdo líquido, inodoro e translúcido. Microscopicamente, observou-se entre a derme média e profunda a formação de múltiplos cistos de tamanho variável. Esses cistos eram revestidos por uma única camada de células predominantemente cubóides. Em alguns focos eram observadas raras projeções papiliformes de epitélio glandular. O citoplasma estava levemente eosinofílico e com raras imagens de decapitação do ápice. O diagnóstico de cistomatose de glândulas apócrinas foi realizado através dos achados macroscópicos e microscópicos.

  1. Dynamics of prolonged salt movement in the Glückstadt Graben (NW Germany) driven by tectonic and sedimentary processes

    Science.gov (United States)

    Warsitzka, Michael; Kley, Jonas; Jähne-Klingberg, Fabian; Kukowski, Nina

    2017-01-01

    The formation of salt structures exerted a major influence on the evolution of subsidence and sedimentation patterns in the Glückstadt Graben, which is part of the Central European Basin System and comprises a post-Permian sediment thickness of up to 11 km. Driven by regional tectonics and differential loading, large salt diapirs, salt walls and salt pillows developed. The resulting salt flow significantly influenced sediment distribution in the peripheral sinks adjacent to the salt structures and overprinted the regional subsidence patterns. In this study, we investigate the geometric and temporal evolution of salt structures and subsidence patterns in the central Glückstadt Graben. Along a key geological cross section, the post-Permian strata were sequentially decompacted and restored in order to reconstruct the subsidence history of minibasins between the salt structures. The structural restoration reveals that subsidence of peripheral sinks and salt structure growth were initiated in Early to Middle Triassic time. From the Late Triassic to the Middle Jurassic, salt movement and salt structure growth never ceased, but were faster during periods of crustal extension. Following a phase from Late Jurassic to the end of the early Late Cretaceous, in which minor salt flow occurred, salt movement was renewed, particularly in the marginal parts of the Glückstadt Graben. Subsidence rates and tectonic subsidence derived from backstripping of 1D profiles reveal that especially the Early Triassic and Middle Keuper times were periods of regional extension. Three specific types of salt structures and adjacent peripheral sinks could be identified: (1) Graben centre salt walls possessing deep secondary peripheral sinks on the sides facing away from the basin centre, (2) platform salt walls, whose main peripheral sinks switched multiple times from one side of the salt wall to the other, and (3) Graben edge pillows, which show only one peripheral sink facing the basin centre.

  2. Sonda permeabilizadora en el tratamiento de la disfunción obstructiva de las glándulas de Meibomio

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    Taimí Cárdenas Díaz

    Full Text Available El ojo seco constituye una de las principales afecciones de la superficie ocular por la que acuden las personas a la consulta de Oftalmología. El 70 % de los pacientes con este diagnóstico presentan algún signo relacionado con la enfermedad de las glándulas de Meibomio y su control con los tratamientos habituales continúa siendo un problema. Las compresas calientes y los lavados con champú infantil darán alivio temporal, pero por lo general son abandonados por los pacientes. Otro enfoque es la expresión de las glándulas de Meibomio, la cual es molesta y por lo general necesita realizarse más de una vez; de ahí que el sondeo quirúrgico de esta sea una alternativa a utilizar. El sondaje mediante las sondas de Maskin introducidas desde el 2009 constituye una nueva modalidad terapéutica, la cual, de acuerdo con el conocimiento de los autores, no había sido introducida previamente en el país. La no disponibilidad de sondas con este fin en Cuba fue la motivación para diseñarla con el objetivo de permeabilizar los conductos excretores en la disfunción obstructiva de las glándulas de Meibomio (O- MGD, y así implementar esta técnica. Este dispositivo se construyó con medios propios en el taller de prototipos del laboratorio, de acuerdo con las características anatómicas del sistema glandular. Actualmente se usa de manera regular por doctores con resultados alentadores, como en los casos que se presentan.

  3. Conduta conservadora no carcinoma papilífero da glândula tireóide

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    Josias de Andrade Sobrinho

    Full Text Available OBJETIVO: Avaliar os resultados do tratamento do carcinoma papilífero limitado a um lobo da glândula tireóide, através de tireoidectomia parcial com seguimento superior a cinco anos. MÉTODO: Estudo retrospectivo de 105 prontuários de pacientes portadores de carcinoma diferenciado da glândula tireóidea operados no Departamento de Cirurgia de Cabeça e Pescoço e Otorrinolaringologia do Hospital Heliópolis, Hosphel, São Paulo de 1977 a 1997 e selecionados 31 casos que apresentavam nódulos limitados a um lobo da glândula com seguimento superior a cinco anos e submetidos à lobectomia total mais istmectomia. RESULTADOS: Observamos somente um caso (3% de disfonia transitória com paresia de prega vocal unilateral que regrediu espontaneamente. Não houve casos de hipoparatireoidismo transitório ou definitivo. O seguimento médio foi de 12,6 anos, sendo todos com seguimento superior a cinco anos; 10 casos (32% com seguimento entre 10 e 15 anos e 10 casos (32% com seguimento além de 15 anos. Não foram observados casos com recorrência loco-regional ou metástase à distância, estando todos eles assintomáticos e sem doença. CONCLUSÃO: Nesta série, a tireoidectomia parcial para carcinoma papilífero limitado a um lobo, mostrou-se eficaz.

  4. Avaliação pós-radioterápica de glândulas salivares maiores

    OpenAIRE

    Galli, Susane D'Arc Barcelos Britto

    2014-01-01

    Objetivo: Este estudo objetivou avaliar a xerostomia por meio de questionário e objetivamente analisou-se o volume das glândulas salivares maiores pela TC e a função e excreção por meio de cintilografia, antes e após a radioterapia. Materiais e Métodos: Neste estudo prospectivo foram selecionados 28 pacientes, 22 homens e 6 mulheres, com idade que variou entre 18 e 75 anos, com diagnóstico histopatológico de carcinoma espinocelular na região de cabeça e pescoço. Os pacientes foram exami...

  5. Real Time Position Detection by Using GPS+GSM+GPRS and Arduino Mega Based Telit GL865

    OpenAIRE

    Baki Koyuncu; ZeynepÖzdemir

    2016-01-01

    A web application on a mobile platform is developed by using GPS, GSMand GPRSon an Arduino Mega board to determine the object positions in real time.The system receives the GPS position data and sends it to a distant server through a GSM channel by using GPRS and an Arduino Mega board. Position data is displayed on a Google map on the server or a mobile cell phone. U BloxLea is deployed as the GPS device, Telit GL865 is used as the combined GSM and GPRS devices. GPS data is received every 1 s...

  6. A new anti-glioma therapy, AG119: pre-clinical assessment in a mouse GL261 glioma model.

    Science.gov (United States)

    Towner, Rheal A; Ihnat, Michael; Saunders, Debra; Bastian, Anja; Smith, Nataliya; Pavana, Roheeth Kumar; Gangjee, Aleem

    2015-07-17

    High grade gliomas (HGGs; grades III and IV) are the most common primary brain tumors in adults, and their malignant nature ranks them fourth in incidence of cancer death. Standard treatment for glioblastomas (GBM), involving surgical resection followed by radiation and chemotherapy with temozolomide (TMZ) and the anti-angiogenic therapy bevacizumab, have not substantially improved overall survival. New therapeutic agents are desperately needed for this devastating disease. Here we study the potential therapeutic agent AG119 in a pre-clinical model for gliomas. AG119 possesses both anti-angiogenic (RTK inhibition) and antimicrotubule cytotoxic activity in a single molecule. GL261 glioma-bearing mice were either treated with AG119, anti-VEGF (vascular endothelial growth factor) antibody, anti c-Met antibody or TMZ, and compared to untreated tumor-bearing mice. Animal survival was assessed, and tumor volumes and vascular alterations were monitored with morphological magnetic resonance imaging (MRI) and perfusion-weighted imaging, respectively. Percent survival of GL261 HGG-bearing mice treated with AG119 was significantly higher (p < 0.001) compared to untreated tumors. Tumor volumes (21-31 days following intracerebral implantation of GL261 cells) were found to be significantly lower for AG119 (p < 0.001), anti-VEGF (p < 0.05) and anti-c-Met (p < 0.001) antibody treatments, and TMZ-treated (p < 0.05) mice, compared to untreated controls. Perfusion data indicated that both AG119 and TMZ were able to reduce the effect of decreasing perfusion rates significantly (p < 0.05 for both), when compared to untreated tumors. It was also found that IC50 values for AG119 were much lower than those for TMZ in T98G and U251 cells. These data support further exploration of the anticancer activity AG119 in HGG, as this compound was able to increase animal survival and decrease tumor volumes in a mouse GL261 glioma model, and that AG119 is also not subject to methyl guanine

  7. Ultraestrutura e função das glândulas salivares de Podisus nigrispinus (Hemiptera: Pentatomidae)

    OpenAIRE

    Castrillon, Luis Carlos Martinez

    2014-01-01

    Podisus nigrispinus Dallas (Hemiptera: Pentatomidae) é um inseto zoofitófago com potencial para controle biológico, pois ninfas e adultos predam diversos insetos, inserindo o aparelho bucal e injetando o conteúdo das glândulas salivares no interior das presas, causando a morte das mesmas. Entretanto, os compostos tóxicos da saliva desse inseto, responsáveis pela morte das presas são ainda desconhecidos. Como primeira etapa para a identificação das possíveis substâncias presentes na saliva de ...

  8. Cystadenoma: a rare tumor originated in minor salivary gland Cistadenoma: um tumor raro em glândula salivar menor

    Directory of Open Access Journals (Sweden)

    Jean Nunes dos Santos

    2008-06-01

    Full Text Available Cystadenoma of salivary glands is an uncommon benign neoplasm that presents intraluminal papillary projections. The authors describe one case of cystadenoma located in the buccal mucosa and highlight its histomorphological features and differential diagnosis.O cistadenoma de glândula salivar é uma neoplasia benigna incomum, que exibe projeções papilíferas intraluminais. Os autores descrevem um caso de cistadenoma localizado na mucosa jugal, discutindo os aspectos histomorfológicos e o diagnóstico diferencial desse tumor.

  9. Alteraciones estructurales de la glándula tiroidea tras la radioterapia Structural alterations of thyroid gland after radiotherapy

    Directory of Open Access Journals (Sweden)

    Caridad Verdecia Cañizares

    2009-03-01

    Full Text Available INTRODUCCIÓN. El objetivo de la presente publicación fue comunicar los casos detectados en nuestro estudio de anomalías estructurales de la glándula tiroidea en pacientes que recibieron radioterapia para el tratamiento de tumores malignos de cabeza y cuello. MÉTODOS. Se realizó un estudio descriptivo de corte transversal para determinar la prevalencia de alteraciones estructurales de la glándula tiroides en 43 pacientes sobrevivientes de tumores malignos de cabeza y cuello, que habían recibido radioterapia y que fueron atendidos en los Hospitales Pediátricos Docentes «William Soler» y «Juan Manuel Márquez» en un período de 10 años (1990 a 2000. RESULTADOS. La alteración estructural de la glándula tiroides más frecuente fue la fibrosis glandular (18,5 %, seguida del adenoma tiroideo y de quistes simples de la glándula (11,1 % respectivamente. Hubo 2 casos de carcinoma diferenciado de la glándula tiroides (7,4 % y uno de lesión mixta adenofolicular (3,7 %. La citología aspirativa con aguja fina permitió la detección de la mayoría de estas lesiones y posibilitó, guiada por ecografía, la detección de lesiones intraparenquimatosas de la glándula. El tratamiento de las alteraciones encontradas fue quirúrgico y se practicó tiroidectomía total a los dos pacientes con carcinoma diferenciado de la glándula tiroides, tiroidectomía subtotal a los tres pacientes con quistes de la glándula y adenomas tiroideos. En los casos de fibrosis glandular se detectó hipofunción tiroidea concomitante, por tanto se administró tratamiento sustitutivo con levotiroxina. CONCLUSIONES. La citología aspirativa con aguja fina guiada mediante ecografía de la glándula tiroides permitió diagnosticar alteraciones estructurales no detectadas en el examen físico. La patología maligna más irradiada correspondió a los linfomas no Hodgkin. Cuanto mayor es la dosis de irradiación, mayor es la patología tiroidea.INTRODUCTION: The aim of

  10. Research for visualization of running state of long-distance water transmission pipeline based on OpenGL

    Science.gov (United States)

    Zhang, Xiaoping; Xu, Xuejun; Liu, Bing; Zhang, Zhendong

    2017-03-01

    The running condition of long distance water pipeline are complicated and changeable, and the lag of water flow is obvious, which is the key technical problem to be solved in the process of regulation and control. According to the present situation of the long-distance water conveyance project, the visualization simulation technology is used to study the operation and management of the long-distance water conveyance pipeline based on OpenGL technology. The system developed in this paper can combine pipeline information, working condition data and relevant data to provide a visualization platform for analysis and decision-making of project management and operation.

  11. Colgajo bilateral de perforantes lumbares para reconstrucción glútea tras infiltración por modelantes

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    J. Gordillo-Hernández

    2014-09-01

    Full Text Available La inyección de sustancias modelantes es un procedimiento usado desde el siglo XIX para mejorar el contorno corporal con fines estéticos. Durante la última década ha aumentado la demanda de técnicas quirúrgicas para la reconstrucción de los defectos originados tras la resección de unidades anatómicas afectadas por infiltración de estas sustancias modelantes, haciendo que en ocasiones tengamos que recurrir al empleo de expansores cutáneos, injertos, colgajos locales, colgajos regionales e incluso, a colgajos libres. Describimos el proceso de reconstrucción glútea en caso de infiltración de sustancias modelantes en el que tuvimos que emplear un colgajo de perforantes lumbares debido a su fiabilidad, a que preserva la musculatura, a su versatilidad de diseño sin comprometer otras posibles alternativas reconstructivas y por su baja morbilidad. Analizamos también la anatomía, diseño, técnica quirúrgica e indicaciones específicas de estos colgajos de perforantes de la región glútea.

  12. Morphologic study of the pineal gland of the dog/ Estudo morfológico da glândula pineal do cão

    Directory of Open Access Journals (Sweden)

    Francisco Xavier Hernandez Blazquez

    2008-08-01

    Full Text Available Following the discovery of the melatonin by Lerner et al. (1958, new research and perspectives were developed in order to improve the knowledge regarding the pineal gland. This hormone is not only related to the circadian control but also influences other behavioral functions such as the reproductive cycle and thermoregulation. In this study the morphology of the pineal gland (epiphysis cerebri from 20 dogs were analyzed by means of macroscopic and microscopic evaluation. The shape of the gland ranged from conic to “tongue-like shape” (in relation to human tongue. The gland color ranged from beige to gray-brownish and it had a gelatinous consistency. The width and length of the glands ranged from 1.38 to 2.39 mm and 1.53 to 2.96 mm, respectively. Capsule, septa, pinealocytes, glial cells and pigment granules were microscopically found in all glands. No calcareous concretions were observed.Após a descoberta da melatonina por Lerner et al. (1958, novas pesquisas e perspectivas surgiram tornando a pineal uma estrutura mais estudada. Além disso, sabe-se que este hormônio está relacionado com o controle circadiano e outras funções comportamentais fisiológicas como o ciclo reprodutivo e termorregulação. Neste trabalho, foram utilizados 20 cães e estudamos a morfologia das glândulas, empregando uma análise macroscópica e microscópica. O formato das pineais variou de cônico a glossóide (em relação à língua humana; cor entre bege e marrom-acinzentado e apresentaram consistência gelatinosa. A largura e o comprimento das glândulas variaram respectivamente de 1,38 a 2,39 mm e de 1,53 a 2,96 mm. Quanto à microscopia, utilizamos coloração de eosina-hematoxilina (HE, e em todas as pineais observamos a presença dos seguintes componentes: cápsula; septos ou trabéculas pinealócitos; células da glia; grânulos de pigmentos, e não foram notadas concreções calcáreas.

  13. Análise histoquímica das glândulas anexas do aparelho reprodutor do macho de paca (Cuniculus paca

    Directory of Open Access Journals (Sweden)

    Aretuza C. Capalbo

    Full Text Available RESUMO: A paca (Cuniculus paca é um roedor típico de regiões tropicais. Com a finalidade de estudar esta espécie selvagem para manejo adequado e sua preservação, objetivou-se neste trabalho caracterizar a histoquímica das glândulas anexas do trato reprodutor do macho da paca. Para este fim, cortes histológicos dessas glândulas foram submetidas às reações histoquímicas com Ácido Periódico de Schiff (PAS, Alcian blue (AB, PAS. + AB. e PAS + Amilase. Na glândula bulbouretral foi constatado que o epitélio produz secreção rica em glicoproteínas neutras e ácidas, glicosaminoglicanas, e em algumas regiões produz mais de um tipo de secreção. Não foi observada a presença de glicogênio no epitélio. Na glândula vesicular, seu epitélio em borda em escova corou-se por glicoproteínas neutras e também por substância de composição desconhecida, não contendo glicoproteínas ácidas, glicogênio ou glicosaminoglicanas. Verificou-se presença de pequena quantidade de glicoproteínas ácidas e neutras na próstata, em especial na mucosa, além de glicoproteínas ácidas carboxiladas e sulfatadas em pequena quantidade no tecido conjuntivo da lâmina própria dessa glândula. Por fim, a glândula coaguladora apresentou pequena quantidade de glicoproteínas neutras na borda em escova de seu epitélio e substância de composição desconhecida, sendo ausente o glicogênio. Conclui-se que as glândulas anexas do trato reprodutor da paca apresentam características histoquímicas que compartilham certa similaridade com outras espécies da ordem Rodentia, com a presença de glicoproteínas neutras e ácidas em algumas glândulas, principalmente no epitélio da glândula bulbouretal e na borda em escova do epitélio das demais glândulas.

  14. Clinical observation on 1g/L bromfenac sodium hydrate ophthalmic solution associated with glucocorticoid in high myopia after LASEK

    Directory of Open Access Journals (Sweden)

    Qing-He Li

    2017-06-01

    Full Text Available AIM:To observe the efficacy and safety of 1g/L bromfenac sodium hydrate ophthalmic solution in the partial substitution of glucocorticoid after laser subepithelial keratomileusis(LASEK. METHODS: Totally 104 cases(208 eyeswere received LASEK, which were selected and divided into study group and control group. The study group were adopted 1g/L bromfenac sodium hydrate ophthalmic solution combined with tobramycin dexamethasone eye drops and fluorometholone eye drops, the control group were adopted tobramycin dexamethasone eye drops and fluorometholone eye drops. The changes of visual acuity and intraocular pressure of two groups were recorded before and after surgery, and the score of painness and the occurrence of haze were observed after surgery. RESULTS: At postoperative 1, 2, 3d, respectively, the pain score of the study group were 1.70±0.35, 1.25±0.34, 0.82±0.32, the pain score of the control group were 2.30±0.43, 1.68±0.44, 1.12±0.33, the differences were significant(PP>0.05, and the differences were not significant(P>0.05. At preoperative and postoperative 1, 2wk, 1 and 3mo of surgery, respectively, the intraocular pressure of study group were 17.33±1.58, 7.54±1.28, 7.23±1.58, 7.26±1.47, and 7.30±1.36 mmHg; the intraocular pressure of control group were 17.53±1.43,7.57±1.32,7.73±1.55,7.80±1.38,7.86±1.43 mmHg,the differences were not significant between before and at 2wk after surgery(P>0.05, the differences were significant between the two groups at 2wk, 1mo and 3mo(PP>0.05. CONCLUSION: It is safe and effective that 1g/L bromfenac sodium hydrate ophthalmic solution in the partial substitution of glucocorticoid after laser subepithelial keratomileusis. The patient has a lower intraocular pressure, has similar therapeutic effect as glucocorticoid in vision and antiinflammatory.

  15. Transplante de glândulas salivares labiais no tratamento de olho seco em cães pela autoenxertia

    Directory of Open Access Journals (Sweden)

    Leticia Séra Castanho

    2013-12-01

    Full Text Available OBJETIVO: Avaliar os efeitos clínicos da secreção das glândulas salivares labiais como alternativa de lubrificação ocular para alívio do olho seco, em casos moderados, severos e refratários ao tratamento clínico, através da técnica de transposição de glândulas salivares labiais para o fórnice conjuntival pela autoenxertia. MÉTODOS: Foram selecionados 17 cães os quais apresentavam olho seco autoimune sem reposta satisfatória ao tratamento clínico. O teste lacrimal de Schirmer e o tempo de ruptura do filme lacrimal foram realizados no pré-operatório para avaliar a quantidade e a qualidade da lágrima produzida. Os pacientes foram submetidos aos exames oftálmicos completos no pré-operatório, a cada 15 dias por dois meses e a cada 30 dias por mais dois meses, totalizando seis retornos pós-operatórios. No pré-operatório e em todos os pós-operatórios fotografias digitais foram tiradas para o arquivo fotográfico. Utilizou-se o programa photoshop para avaliação e marcação dos neovasos corneanos em todos os retornos. RESULTADOS: Houve redução em todos os casos da secreção mucopurulenta, hiperemia conjuntival e blefarospasmo, bem como estabilização de lesões pré-existentes e redução importante do número de neovasos corneanos. A transposição resultou na melhora do tempo de ruptura do filme lacrimal, porém sem alterações significativas no teste de Schirmer. CONCLUSÃO: O transplante das glândulas salivares labiais para o fórnice conjuntival é um procedimento de fácil execução, rápido, eficaz, acessível a qualquer cirurgião veterinário oftalmologista e de grande valia para casos moderados e severos de ceratoconjuntivite seca não responsivos às medicações existentes.

  16. La glándula uropigia de aves de distintos ambientes: su estructura y función

    OpenAIRE

    Chiale, María Cecilia

    2016-01-01

    La glándula uropigia de las aves es un órgano sebáceo compacto ubicado dorsalmente sobre las últimas vértebras caudales. Es muy variable en cuanto a su forma y tamaño pero, generalmente, está formada por dos lóbulos y en su extremo caudal posee una papila; en ésta última región se encuentran los poros excretores que suelen ser dos en la mayoría de las aves, pero en algunos casos se observó que éste número puede ser mayor. También es posible encontrar un pincel de plumas rodeando los poros exc...

  17. Revisión bibliográfica de los tumores de la glándula lagrimal

    OpenAIRE

    Bañón Navarro, Raquel

    2015-01-01

    Analizar la patología tumoral de la Glándula Lagrimal en su extensión clínica, diagnóstica, terapéutica y vital. MATERIAL Y MÉTODOS La literatura científica en esta revisión bibliográfica procede de las bases de información médica LILACS, SCIENCEDIRECT, MEDLINE Y COCHRANE. Los Medical Subject Headings fueron “lacrimal gland” y “tumor lacrimal gland”. Los artículos científicos seleccionados fueron valorados por el cuestionario CASPe y la declaración de STROBE para valor...

  18. Alergia ao glúten: série de nove casos = Gluten allergy: series of nine cases

    Directory of Open Access Journals (Sweden)

    Maia, Estefânia Barrosa

    2014-01-01

    Conclusões: A alergia ao glúten é rara, explicando o pequeno número diagnosticado em 15 anos, em um hospital central de referência. Os nove casos tiveram início no primeiro ano de vida, sendo as manifestações cutâneas as mais frequentes. O eczema foi uma apresentação frequente e a sensibilização foi por via cutânea em alguns casos. A alergia alimentar múltipla também foi frequente nos casos descritos. Três das nove crianças resolveram a alergia até os seis anos, tendo em comum uma manifestação inicial cutânea. Nestes nove casos, o valor de IgE específica não foi preditivo da evolução clínica

  19. Efeito da substituição da farinha de trigo no desenvolvimento de biscoitos sem glúten

    Directory of Open Access Journals (Sweden)

    Tamires dos Santos VIEIRA

    2015-12-01

    Full Text Available Resumo O glúten pode determinar a qualidade da farinha de trigo, conferindo às massas características como elasticidade e capacidade de absorver água, o que torna a elaboração de produtos de panificação isentos de glúten um desafio. Desse modo, o presente estudo buscou avaliar as características físico-químicas e sensoriais de biscoitos elaborados com ingredientes sucedâneos ao trigo, como fécula de mandioca, farinha de soja, de quinoa e de amaranto. Para a caracterização física foram determinados: peso, diâmetro, espessura, fator de expansão e volume específico. Quanto à composição química foram determinados: umidade, cinzas, proteínas, lipídios, fibras alimentares, carboidratos totais, energia, além de pH e acidez. A aceitabilidade foi determinada por meio de uma escala hedônica de nove pontos. As formulações contendo farinhas sucedâneas ao trigo apresentaram bom rendimento, maior teor de cinzas, de proteínas, de lipídios e de fibras alimentares. Também, essas apresentaram percentuais de aceitabilidade superiores a 70%, exceto para a avaliação do sabor da amostra com maior teor de soja (40 g/100g. Conclui-se que as formulações contendo farinhas sucedâneas ao trigo podem ser consideradas promissoras, disponibilizando alternativas alimentares àqueles que almejam produtos com valor nutricional superior e posicionamento único no mercado, visto que os produtos disponíveis muitas vezes possuem baixo valor nutritivo ao serem elaborados com farinhas amiláceas.

  20. Salinivibrio costicola GL6, a Novel Isolated Strain for Biotransformation of Caffeine to Theobromine Under Hypersaline Conditions.

    Science.gov (United States)

    Ashengroph, Morahem

    2017-01-01

    The present study has been conducted towards isolation of moderately halophilic bacteria capable of transforming caffeine into theobromine. A total of 45 caffeine-degrading moderate halophiles were enriched from hypersaline lakes and examined for the biotransformation of caffeine to theobromine by thin-layer chromatography (TLC) and high-performance liquid chromatography analyses. Strain GL6, giving the highest yield of theobromine, was isolated from the Hoz Soltan Lake, 20 % w/v salinity, central Iran, and identified as Salinivibrio costicola based on morphological and biochemical features as well as its 16S rRNA gene sequence analysis (GeneBank Accession No. KT378066) and DNA-DNA relatedness. The biotransformation of caffeine with strain GL6 leads to the formation of two metabolites, identified as theobromine and paraxanthine, but the yield of paraxanthine was much lower. Further study on the production of theobromine from caffeine under resting cell experiment was carried out subsequently. The optimal yield of theobromine (56 %) was obtained after a 32-h incubation using 5 mM of caffeine and 15 g l-1 (wet weight) of biomass in 0.1 M saline phosphate buffer (pH 7.0 and 10 % w/v NaCl) under agitation 180 rpm at 30 °C. The biotransformed theobromine was purified by preparative TLC and subjected to FTIR and mass spectroscopy for chemical identification. This is the first evidence for biotransformation of caffeine into theobromine by strains of the genus Salinivibrio.

  1. Epstein-Barr Virus Fusion with Epithelial Cells Triggered by gB Is Restricted by a gL Glycosylation Site.

    Science.gov (United States)

    Möhl, Britta S; Chen, Jia; Park, Seo Jin; Jardetzky, Theodore S; Longnecker, Richard

    2017-12-01

    Epstein-Barr virus (EBV) entry into epithelial cells is mediated by the conserved core fusion machinery, composed of the fusogen gB and the receptor-binding complex gH/gL. The heterodimeric gH/gL complex binds to the EBV epithelial cell receptor or gp42, which binds to the B-cell receptor, triggering gB-mediated fusion of the virion envelope with cellular membranes. Our previous study found that the gL glycosylation mutant N69L/S71V had an epithelial cell-specific hyperfusogenic phenotype. To study the influence of this gL mutant on the initiation and kinetics of gB-driven epithelial cell fusion, we established a virus-free split-green fluorescent protein cell-cell fusion assay that enables real-time measurements of membrane fusion using live cells. The gL_N69L/S71V mutant had a large increase in epithelial cell fusion activity of up to 300% greater than that of wild-type gL starting at early time points. The hyperfusogenicity of the gL mutant was not a result of alterations in complex formation with gH or alterations in cellular localization. Moreover, the hyperfusogenic phenotype of the gL mutant correlated with the formation of enlarged syncytia. In summary, our present findings highlight an important role of gL in the kinetics of gB-mediated epithelial cell fusion, adding to previous findings indicating a direct interaction between gL and gB in EBV membrane fusion.IMPORTANCE EBV predominantly infects epithelial cells and B lymphocytes, which are the cells of origin for the EBV-associated malignancies Hodgkin and Burkitt lymphoma as well as nasopharyngeal carcinoma. Contrary to the other key players of the core fusion machinery, gL has the most elusive role during EBV-induced membrane fusion. We found that the glycosylation site N69/S71 of gL is involved in restricting epithelial cell fusion activity, strongly correlating with syncytium size. Interestingly, our data showed that the gL glycosylation mutant increases the fusion activity of the hyperfusogenic g

  2. A dioxin response element in the multiple cloning site of the pGL3 luciferase reporter influences transcriptional activity1

    Science.gov (United States)

    Ochs, Sharon; Liu, Jing; Fernando, Tharu; Fecher, Roger; Sulentic, Courtney E.W.

    2012-01-01

    Luciferase reporter plasmids (pGL3 backbone, Promega) have been utilized to characterize the transcriptional effects of the environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and other aryl hydrocarbon receptor (AhR) ligands. Following ligand activation, the AhR and its dimerization partner AhR nuclear translocator (ARNT) regulate transcription by binding dioxin response elements (DREs) in regulatory regions of dioxin-sensitive genes. Upon sequencing of our luciferase reporters, we unexpectedly identified a DRE core motif within the multiple cloning site (mcsDRE) of the pGL3 luciferase plasmid backbone in a subset of our reporters. Therefore, the objective of this study was to determine if the mcsDRE inadvertently influences reporter activity. Utilizing deletional analysis we determined that the mcsDRE did significantly alter the transcriptional effect induced by TCDD. Since many chemicals have been shown to interact with the AhR and influence transcription through the DRE, the presence of the mcsDRE in the pGL3 luciferase plasmid may inappropriately influence promoter and enhancer analysis. As such, insertion of regulatory elements into pGL3 reporters should be designed to avoid retaining the mcsDRE core motif (GCGTG) and currently utilized pGL3 reporters should be evaluated for the presence of the mcsDRE. PMID:22652426

  3. Análise histoquímica das glândulas anexas do aparelho reprodutor do macho de paca (Cuniculus paca)

    OpenAIRE

    Aretuza C. Capalbo; Claudinei Cruz; Fabrício S. Oliveira; Sasahara,Tais H.C.; Leonardo M. Leal; LUCIANA S. SIMÕES; Miglino, Maria A; Machado, Márcia R.F.

    2016-01-01

    RESUMO: A paca (Cuniculus paca) é um roedor típico de regiões tropicais. Com a finalidade de estudar esta espécie selvagem para manejo adequado e sua preservação, objetivou-se neste trabalho caracterizar a histoquímica das glândulas anexas do trato reprodutor do macho da paca. Para este fim, cortes histológicos dessas glândulas foram submetidas às reações histoquímicas com Ácido Periódico de Schiff (PAS), Alcian blue (AB), PAS. + AB. e PAS + Amilase. Na glândula bulbouretral foi constatado qu...

  4. The muscle-specific protein phosphatase PP1G/R(GL)(G(M))is essential for activation of glycogen synthase by exercise

    DEFF Research Database (Denmark)

    Aschenbach, W G; Suzuki, Y; Breeden, K

    2001-01-01

    that was originally postulated to mediate insulin control of glycogen metabolism. However, we recently showed (Suzuki, Y., Lanner, C., Kim, J.-H., Vilardo, P. G., Zhang, H., Jie Yang, J., Cooper, L. D., Steele, M., Kennedy, A., Bock, C., Scrimgeour, A., Lawrence, J. C. Jr., L., and DePaoli-Roach, A. A. (2001) Mol....... Cell. Biol. 21, 2683-2694) that insulin activates GS in muscle of R(GL)(G(M)) knockout (KO) mice similarly to the wild type (WT). To determine whether PP1G is involved in glycogen metabolism during muscle contractions, R(GL) KO and overexpressors (OE) were subjected to two models of contraction...... of glycogen metabolism under basal conditions and in response to contractile activity, and may explain the reduced muscle glycogen content in the R(GL) KO mice, despite the normal insulin activation of GS....

  5. Efeitos do som basal em fendas glóticas Effects of vocal fry incomplete glottal closure

    Directory of Open Access Journals (Sweden)

    Geovana de Paula Bolzan

    2008-01-01

    Full Text Available TEMA: som basal em fendas glóticas. PROCEDIMENTOS: participaram desta pesquisa dois sujeitos do sexo feminino, com idades entre 20 e 40 anos e diagnóstico otorrinolaringológico de fenda em ampulheta. Houve gravação da emissão sustentada da vogal /a/ e exame videolaringoestroboscópico, imediatamente a seguir, os sujeitos realizaram o som basal em três séries de 15 repetições, e foram submetidos a novo exame laríngeo e gravação da vogal. Os dados pré e pós-realização do som basal foram submetidos às analises acústica, perceptivo-auditiva e videolaringoestroboscópica, realizadas por juízes (três fonoaudiólogas e três otorrinolaringologistas, respectivamente. RESULTADOS: em ambos os sujeitos, houve melhora no fechamento glótico e amplitude de vibração da mucosa das pregas vocais; piora no tipo de voz; aumento das medidas de ruído e de Jitter. CONCLUSÃO: o som basal promoveu redução das fendas glóticas e aumento da amplitude de vibração da mucosa das pregas vocais; piora do tipo de voz, que ficou mais ruidoso; aumento das medidas de ruído e de Jitter, sugerindo irregularidade vibratória, provavelmente devido ao efeito do ajuste do som basal ao mobilizar intensamente a mucosa.BACKGROUND: vocal fry in incomplete glottal closure. PROCEDURE: two individuals aged between 20 and 40 years old presenting an otolaryngological diagnosis of ampoule chink were part of the study. A recording of the sustained emission of /a/ vowel took place, as well as a videostroboscopic examination. Right after, the individuals completed the vocal fry in three series of 15 repetitions, being submitted to a new laryngeal examination and to the vowel’s recording. Both pre-vocal and post vocal fry data were assessed through acoustic, perceptive-auditive and videostroboscopic analysis, carried out by judges (three speech and language pathologists and three otolaryngologists, respectively. RESULTS: for both individuals, there was an improvement

  6. Gene expression profiling in the lungs of pigs with different susceptibilities to Glässer's disease

    Directory of Open Access Journals (Sweden)

    Sargent Carole A

    2010-07-01

    Full Text Available Abstract Background Haemophilus parasuis is the causative agent of Glässer's disease in pigs. Currently, little is known about the molecular mechanisms that contribute to disease susceptibility. This study used a porcine oligonucleotide microarray to identify genes that were differentially expressed (DE in the lungs of colostrum-deprived animals previously characterized as being either 'Fully Resistant' (FR or 'Susceptible' to infection by H. parasuis in a bacterial challenge experiment. Results Gene expression profiles of 'FR' and 'Susceptible' animals were obtained by the identification of genes that were differentially expressed between each of these groups and mock-inoculated 'Control' animals. At 24 hours post-inoculation, a total of 21 and 58 DE genes were identified in 'FR' and 'Susceptible' animals respectively. At 72 hours, the numbers of genes were 20 and 347 respectively. 'FR' animals at 24 hours exhibited an increased expression of genes encoding extracellular matrix and TGF-β signalling components, possibly indicative of tissue repair following the successful early resolution of infection. The gene expression profile of 'FR' animals at 72 hours supported the hypothesis that higher levels of antibacterial activity were responsible for the 'FR' phenotype, possibly due to an increase in natural immunoglobulin A and decrease in signalling by the immunoregulatory transcription factor peroxisome proliferator-activated receptor gamma (PPAR-γ. The expression profile of 'Susceptible' animals at both time-points was characterized by an imbalance in signalling between pro and anti-inflammatory cytokines and an increased expression of genes involved in biological processes associated with inflammation. These include the pro-inflammatory cytokine genes resistin (RETN and interleukin 1-beta (IL1B. At 72 hours, a reduction in the expression of genes involved in antigen presentation by both MHC class I and II molecules was observed, which could

  7. Análisis ultraestructural de membranas de glóbulos grasos lácteos de calostro y leche humana

    OpenAIRE

    Hernández, Francisco; Akahori, Hiroshi

    2016-01-01

    Se describen ultraestructuralmente los glóbulos grasos lácteos y sus membranas, a partir de leche y calostro de origen humano. Al microscopio electrónico de barrido los glóbulos aparecen como estructuras esferoides con algunas irregularidades en su superficie. Al micriscopio electrónico de transmisión se ve que esas irregularidades están compuestas por islotes de un material morfológicamente similar al citoplasma, en el que aparecen estructuras similares a fragmentos de retículo endoplasmátic...

  8. Sialolitos en conductos y glándulas salivales: Revisión de literatura Sialoliths in ducts and salivary glands: Literature review

    OpenAIRE

    M. Rebolledo Cobos; Z. Carbonell Muñoz; A. Díaz Caballero

    2009-01-01

    La sialolitiasis es una afección que se produce por la obstrucción de una glándula salival o de su conducto excretor por la formación de concreciones calcáreas o sialolitos en el parénquima de los mismos. Existen teorías que afirman que los sialolitos en las glándulas y conductos salivales son originados por la mineralización de varios componentes como: cuerpos extraños, detritus celulares y microorganismos, depositándose inicialmente una matriz orgánica, probablemente de glucoproteínas, para...

  9. Reanimación con glóbulos rojos Rh positivo y sin prueba cruzada en emergencias médicas.

    OpenAIRE

    Armando Corté; Wilson Wedekin; Fabiola Bolaños

    2009-01-01

    INTRODUCCIÓN: Se presentan tres años de experiencia en el uso de concentrado de glóbulos rojos O Rh positivo sin cruzar para transfusión de emergencia en pacientes severamente lesionados e hipovolémicos en una clínica privada de Cali. MATERIALES Y MÉTODOS: La sangre se solicitó para pacientes con trauma, hemorragia intraoperatoria masiva inesperada, o ruptura de aneurisma. Los glóbulos rojos O Rh positivos es la primera elección para el soporte de pacientes que no pueden esperar sangre tipo ...

  10. The energy blockers bromopyruvate and lonidamine lead GL15 glioblastoma cells to death by different p53-dependent routes.

    Science.gov (United States)

    Davidescu, Magdalena; Macchioni, Lara; Scaramozzino, Gaetano; Cristina Marchetti, Maria; Migliorati, Graziella; Vitale, Rita; Corcelli, Angela; Roberti, Rita; Castigli, Emilia; Corazzi, Lanfranco

    2015-09-21

    The energy metabolism of tumor cells relies on aerobic glycolysis rather than mitochondrial oxidation. This difference between normal and cancer cells provides a biochemical basis for new therapeutic strategies aimed to block the energy power plants of cells. The effects produced by the energy blockers bromopyruvate (3BP) and lonidamine (LND) and the underlying biochemical mechanisms were investigated in GL15 glioblastoma cells. 3BP exerts early effects compared to LND, even though both drugs lead cells to death but by different routes. A dramatic decrease of ATP levels occurred after 1 hour treatment with 3BP, followed by cytochrome c and hexokinase II degradation, and by the decrease of both LC3I/LC3II ratio and p62, markers of an autophagic flux. In addition, Akt(Ser(473)) and p53(Ser(15)/Ser(315)) dephosphorylation occurred. In LND treatment, sustained ATP cellular levels were maintained up to 40 hours. The autophagic response of cells was overcome by apoptosis that was preceded by phosphatidylinositol disappearance and pAkt decrease. This last event favored p53 translocation to mitochondria triggering a p53-dependent apoptotic route, as observed at 48 and 72 hours. Adversely, in 3BP treatment, phospho-p53 dephosphorylation targeted p53 to MDM2-dependent proteolysis, thus channeling cells to irreversible autophagy.

  11. Tumores de glándulas salivales: Su comportamiento en 10 años de trabajo (1993-2002

    Directory of Open Access Journals (Sweden)

    Oscar García-Roco Pérez

    2003-12-01

    Full Text Available Se realizó un estudio descriptivo de 107 pacientes que fueron intervenidos quirúrgicamente debido a afecciones en glándulas salivales, durante un período de 10 años, en el Servicio de Cirugía Maxilofacial del Hospital Provincial Docente "Manuel Ascunce Domenech" de Camagüey, con el propósito de describir su frecuencia según edad y sexo, comportamiento biológico, localización anatómica y resultado histopatológico. Hubo predominio de las condiciones neoplásicas benignas en la parótida, mientras que en la submandibular se manifestaron con mayor frecuencia los procesos malignos. El adenoma pleomorfo fue el tumor más frecuente entre las neoplasias benignas y el carcinoma mucoepidermoide, el más común entre las malignas.A descriptive study of 107 patients, who had been surgically treated from salivary gland tumors in the Maxillofacial Surgery Department of "Manuel Ascunce Domenech" provincial teaching hospital located in Camagüey during 10 years, was performed to describe the sex and age distribution and frequency of these tumors, their biological behavior, anatomical location and histopathological outcome. It was observed that benign neoplasias predominated in the parotid glands whereas malignant processes developed more often in submandibular glands. Pleomorphic adenoma was the most found tumor among the benign neoplasias and mucoepidermoid carcinoma was the commonest among the malignant processes.

  12. Pollen grains in quaternary sediments from the Campos Basin, state of Rio de Janeiro, Brazil: Core BU-91-GL-05

    Directory of Open Access Journals (Sweden)

    Aline Gonçalves de Freitas

    2013-12-01

    Full Text Available Here, we describe pollen grains extracted from a Pleistocene-Holocene sediment core (BU-91-GL-05; 22°48'45"S; 41°54'13"W taken from the Albacora Slope (22°48'45"S; 41°54'13"W, located in the Campos Basin of the northern region of the state of Rio de Janeiro, Brazil. The analysis resulted in the identification and morphological description of 46 types of pollen: one of a gymnosperm genus (Podocarpus; and 45 of angiosperm taxa across 27 families-one family of monocotyledons (Poaceae and 26 families (30 types of dicotyledons. The most common angiosperm families were Amaranthaceae (Chenopodium, Amaranthus and Gomphrena; Fabaceae (Fabaceae type, Bauhinia, Inga and Canavalia; Malpighiaceae (Tetrapteris, Heteropteris and Peixotoa; Malvaceae (Sida, Abutilon, Hibiscus and Pseudobombax; Rubiaceae (Faramea, Borreria and Psychotria; Asteraceae (Eupatorium and tribe Vernonieae; Bignoniaceae (Bignoniaceae type, Adenocalymma and Tabebuia; and Onagraceae (Fuchsia and Ludwigia. The palynoflora in this study are associated with dense montane and submontane Atlantic Forest, semideciduous forest and restinga (coastal woodland, all of which are present in the state of Rio de Janeiro. Palynological analysis can provide important data about paleovegetation and paleoclimatic changes in the studied area during the Quaternary, specifically in the last 145,000 years.

  13. Effect of maternal antibodies and pig age on the antibody response after vaccination against Glässers disease.

    Science.gov (United States)

    Pomorska-Mól, Małgorzata; Markowska-Daniel, Iwona; Rachubik, Jarosław; Pejsak, Zygmunt

    2011-08-01

    The influence of age and maternal antibodies on the development and duration of postvaccinal antibody response against Glässer's disease were investigated. Pigs born to immune (MDA-positive) and non-immune (MDA-negative) sows were vaccinated with inactivated vaccine. Vaccination was done according to three different protocols: at 1 and 4, at 2 and 5 or at 4 and 7 weeks of age. There were also two control groups for MDA-negative and MDA-positive pigs. The level of Haemophilus parasuis (Hps) specific antibodies were determined using commercial ELISA test. No serological responses were seen in any of the groups after the first vaccination. Maternally derived antibodies (MDA) against Hps were above the positive level until approximately 3 weeks of life in MDA-positive pigs. In those pigs the strongest postvaccinal humoral response was observed in piglets vaccinated at 4 and 7 weeks of age. In the remaining MDA-positive piglets only slight seroconversion was noted but levels of antibodies never exceeded values considered as positive. All MDA-negative pigs produced Hps-specific antibodies after the second vaccination. The results of the present study indicated that MDA may alter the development and duration of active postvaccinal antibody response. Age of pigs at the moment of vaccination was not associated with the significant differences in the magnitude of antibody response, however influenced the kinetics of decline of Hps-specific antibodies.

  14. Achado incidental de glândula sebácea em colo uterino: provavelmente um processo metaplásico

    OpenAIRE

    Ogata,Daniel Cury; Pereira Neto,Elisiário; Perin, Daiana Paola; May, Bruna Miers

    2012-01-01

    As glândulas sebáceas ectópicas são achados muito infrequentes no trato genital feminino. Descrevemos um caso que ocorreu em paciente de 42 anos de idade, submetida à histerectomia total, devido à leiomiomatose e ao prolapso uterino.

  15. Efeito do isostretching na resistência muscular de abdominais, glúteo máximo e extensores de tronco, incapacidade e dor em pacientes com lombalgia

    Directory of Open Access Journals (Sweden)

    Christiane de Souza Guerino Macedo

    Full Text Available Objetivou-se determinar a influência do método Isostretching na resistência muscular do glúteo máximo, abdominais e extensores de tronco, incapacidade e dor de pacientes com lombalgia. MATERIAL E MÉTODOS: Este estudo caracterizou-se como um ensaio clínico aleatório, constituído de 18 mulheres, entre 18 e 25 anos, com dor cinco ou mais na escala visual análoga (EVA na região lombar, no mínimo três vezes por semana, randomizadas em grupo ativo e controle. Foram excluídas as submetidas a tratamentos fisioterápicos ou que tenham passado por cirurgia recente e com outras patologias. As pacientes foram avaliadas (avaliadores cegos no início, após 10 e 20 sessões, pelo questionário Roland-Morris, EVA e Teste de Repetição Máxima em um minuto para abdominais, extensores de tronco e glúteo máximo. Para a análise estatística, foi aplicado o teste de Shapiro Wilk, Teste t de Student e ANOVA. RESULTADOS: A comparação entre os valores iniciais e finais no grupo ativo para a análise da resistência dos músculos abdominais, glúteo máximo, extensores de tronco, incapacidade e dor apresentou p = 0,00 e no grupo controle p = 0,02 para o glúteo máximo. A análise dos três momentos de avaliação no grupo ativo evidenciou para a resistência dos músculos abdominais, glúteo máximo e dor p = 0,00, incapacidade p = 0,01 e para os extensores de tronco p = 0,80 e o grupo controle não evidenciou alteração. CONCLUSÃO: O Isostretching mostrou-se eficiente para diminuir a incapacidade e dor, bem como para o aumento da resistência muscular de abdominais, glúteo máximo e extensores de tronco de pacientes com lombalgia.

  16. Targeting beta-catenin signaling to induce apoptosis in human breast cancer cells by z-guggulsterone and Gugulipid extract of Ayurvedic medicine plant Commiphora mukul.

    Science.gov (United States)

    Jiang, Guoqin; Xiao, Xiao; Zeng, Yan; Nagabhushanam, Kalyanam; Majeed, Muhammed; Xiao, Dong

    2013-08-03

    z-Guggulsterone (z-Gug) and Gugulipid (GL) have been used to treat a variety of ailments. We now report their anti-cancer effect and mechanism against human breast cancer. Using the human estrogen receptor-positive (MCF-7) and triple-negative (MDA-MB-231) breast cancer cells as well as the normal human mammary epithelial cell line (HMEC), we evaluated the anti-breast-cancer efficacy and apoptosis inducing activity of GL. We determined the cellular and molecular mechanism of GL-inhibited breast cancer cell growth. GL significantly inhibited growth of MCF-7 and MDA-MB-231 cells with an IC50~2 μM at pharmacologically relevant concentrations standardized to its major active constituent z-Gug. The GL-induced growth inhibition correlated with apoptosis induction as evidenced by an increase in cytoplasmic histone-associated DNA fragmentation and caspase 3 activity. The GL-induced apoptosis was associated with down-regulation of the β-Catenin signaling pathway. The decreased expression of Wnt/β-Catenin targeting genes, such as cyclin D1, C-myc and survivin, and the inhibition of the activity of the transcription factor (T-cell factor 4, TCF-4) were observed in GL-treated breast cancer cells. The GL treatment resulted in a significant reduction of β-Catenin /TCF-4 complex in both of the cancer cells. The GL-induced apoptotic cell death was significantly enhanced by RNA Interference of β-Catenin and TCF-4. On the other hand, the normal human mammary epithelial cell HMEC, compared with the human breast cancer cells, is significantly more resistant to growth inhibition and apoptosis induction by GL. The present study indicates that the β-Catenin signaling pathway is the target for GL-induced growth inhibition and apoptosis in human breast cancer.

  17. Aspectos generales de la histología de la glándula salival de las garrapatas con énfasis en Boophilus microplus (Canestrini, 1887

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    César Mora Hernández.

    2000-06-01

    Full Text Available El artículo presenta elementos de histología de las glándulas salivales de los artrópodos plagas. Se plantea que si bien las glándulas salivales han sido estudiadas en detalle, aún se sabe poco sobre los mecanismos de transmisión de agentes patógenos, y acerca de las sustancias biológicamente activas que mantienen al "huésped" altamente sensible y posibilitan de esta forma la entrada de otros patógenos no relacionados con vectores. El conocimiento que aportan el estudio de estos procesos permite la elaboración de programas de control epidemiológicos eficientes.

  18. Meibografía. Nueva tecnología para la evaluación de las glándulas de Meibomio

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    Manuel Garza-Leon

    2017-07-01

    Full Text Available La meibografía es una tecnología que nos permite evaluar la morfología de las glándulas de Meibomio in vivo de una forma no invasiva y con pocas o nulas molestias. Nuevas tecnologías en el mercado nos permiten realizarla sin necesidad de tocar al paciente y con la ayuda de programas computacionales es posible evaluar de forma objetiva y cuantitativa la pérdida de las mismas. Otras tecnologías de imagenología como la microscopia confocal pueden además analizar el tejido celular adyacente a las glándulas de Meibomio para monitorizar la respuesta al tratamiento instituido. La presente revisión analiza la meibografía desde sus inicios y los avances más recientes en esta área.

  19. Carcinoma mioepitelial de glándula submaxilar. Caso clínico y revisión de la literatura

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    Alejandro Encinas Bascones

    2015-10-01

    Full Text Available El carcinoma mioepitelial es de aparición rara en la glándula submaxilar. Presentamos un caso clínico y una revisión de la literatura que nos permiten establecer un diagnóstico diferencial y un tratamiento óptimo de este tumor. La histología y la inmunohistoquímica desempeñan un papel importante en el diagnóstico definitivo.

  20. Tumores sincrónicos de glándula salivar: A propósito de dos casos Synchronous salivary gland tumors with regard to two cases

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    J. Azúa-Romeo

    2005-06-01

    Full Text Available Presentamos dos casos de pacientes con tumores sincrónicos de glándula salivar. El primero se refiere a dos tumores benignos en la misma glándula parótida, mientras que el segundo caso se trata de una lesión maligna y otra benigna en glándulas parótida y submaxilar ipsilaterales. Los dobles tumores sincrónicos homolaterales de glándula salivar son muy infrecuentes, suponiendo menos del 0,3% de los tumores de esta localización. La bibliografía revisada refiere 42 casos entre los que sólo 9 presentan un tumor maligno y otro benigno. El diagnóstico citológico preoperatorio, junto con una exhaustiva inspección macroscópica intraoperatoria son las claves para detectar los segundos tumores, habitualmente más pequeños y no detectados en estudios previos, y así adecuar el procedimiento terapéutico.We present two new cases of synchronous tumors of the salivary gland. The first one refers to two benign tumors in the same parotid gland, while second one shows a benign lesion and a malignant tumor in two ipsilateral salivary glands. The appearance of synchronous multiple unilateral parotid tumors of different histologic types is extremely rare, accounting for less than 0.3%; this is usually a Warthin´s Tumor associated to other tumors. The current literature has been reviewed and 42 cases have been found entailing two different parotid tumors coexisting. Of these 11 referred to adenolymphoma associated with pleomorphic adenoma. Preoperative cytological diagnosis, as well as an exhaustive intraoperative inspection are the clues to discover nonidentified second tumors.

  1. Estudo morfológico da glândula pineal de Procyon cancrivorus (Cuvier, 1798 (mão-pelada

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    Leandro de Oliveira Marques

    2010-04-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2010v23n2p163 O Procyon cancrivorus é um carnívoro silvestre amplamente distribuído e relativamente comum, mas ainda é pouco estudado, existindo poucos trabalhos relatando sobre a biologia dessa espécie. Este trabalho tem como objetivo, caracterizar morfologicamente a glândula pineal de Procyon cancrivorus, através de estudos macro, microscópicos, radiográficos e compará-los com outros animais já estudados. Foram utilizados quatro animais adultos de ambos os sexos, provenientes do Criatório Científico CECRIMPAS, IBAMA (Processo nº 02027.003731/04-76. Macroscopicamente, a glândula pineal de Procyon cancrivorus foi localizada entre os lobos occipitais dos hemisférios cerebrais e cranialmente ao vermis cerebelar, posicionava-se rostralmente aos colículos rostrais e caudal à comissura das habênulas. Microscopicamente, a glândula é revestida externamente por uma cápsula derivado da meninge pia-máter. Foi notada a presença de três tipos de células no parênquima glandular: pinealócitos, células gliais e mastócitos. Não foram encontradas concreções calcáreas na glândula pineal nos estudos radiográficos e microscópicos.

  2. Morfologia e histoquímica das glândulas sexuais acessórias de Metachirus nudicaudatus, Didelphidae-Marsupialia

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    Suely F. Costa

    Full Text Available Resumo: São descritas a morfologia e a distribuição de glicogênio e mucossubstâncias na próstata e nas glândulas bulbouretrais de Metachirus nudicaudatus (Geoffroy, 1803, única espécie do gênero. A próstata é envolvida pelas túnicas adventícia e muscular, e o estroma é formado pelo conjuntivo da mucosa uretral. O parênquima é constituído pelos túbulos secretores, disseminados na mucosa uretral, e que diferem histológica e histoquimicamente nos segmentos cranial, médio e caudal. Essas diferenças morfo-histoquímicas também são observadas nas partes externa, média e interna de cada túbulo. De um modo geral, os três segmentos prostáticos secretam mucossubstâncias neutras, porém o segmento caudal produz também glicogênio. Os três pares de glândulas bulbouretrais (laterais, intermédias e mediais do Metachirus são envolvidas por uma cápsula conjuntiva e músculo estriado esquelético. O maior par é a bulbouretral lateral que é constituída por longos túbulos secretores de mucossubstâncias neutras. As bulbouretrais intermédias são formadas por túbulos ramificados, que produzem mucossubstâncias neutras, ácidas carboxiladas e ácidas sulfatadas. Os túbulo-ácinos ramificados das bulbouretrais mediais secretam mucossubstâncias neutras. O Metachirus não possui glândulas ampulares, vesículas seminais nem glândulas de coagulação.

  3. Da-Cruz Triple Tool: cómo hacer simple y precisa la cirugía de implante glúteo intramuscular

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    Luis Da-Cruz

    Full Text Available El Da-Cruz Triple Tool es un instrumento multifuncional diseñado para la colocación de implantes glúteos intramusculares. Tiene 3 funciones; a saber: disector, separador y medidor. Consiste en 2 piezas para su uso articulado o por separado. Como disector logra la profundidad y el grosor deseables del músculo glúteo mayor a través de maniobras precisas que provocan menos trauma; por consiguiente, ayuda a obtener una mejor recuperación y resultados. Al mismo tiempo faculta para elevar los espacios abiertos y controlar adherencias o sangrados. La función de medidor permite calcular la dimensión cefalocaudal y la anchura del espacio para escoger el implante adecuado. Con el entrenamiento adecuado en el uso de esta herramienta y la implementación de medidas pre, trans y postoperatorias bien definidas, cualquier cirujano plástico puede obtener resultados consistentemente exitosos en la colocación de implantes glúteos intramusculares.

  4. Tumor glômico endobrônquico com atelectasia de lobo superior direito Bronchial glomus tumor with right upper lobe atelectasis

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    Artur Eugênio de Azevedo-Pereira

    2010-06-01

    Full Text Available Tumores glômicos são raras neoplasias benignas de tecidos moles. Relatamos o caso de um paciente de 32 anos de idade que apresentava atelectasia em lobo superior direito devido a um tumor endobrônquico. O paciente foi submetido a lobectomia superior direita e broncoplastia em cunha. O diagnóstico patológico foi de tumor glômico endobrônquico. Até onde sabemos, essa apresentação com atelectasia em lobo superior direito nunca foi relatada. O tumor glômico deve ser considerado no diagnóstico diferencial de lesões endobrônquicas que causam atelectasia lobar.Glomus tumors are uncommon benign soft tissue neoplasms. We report the case of a 32-year-old male who presented with right upper lobe atelectasis due to an endobronchial tumor. The patient underwent right upper lobectomy and wedge bronchoplasty. The pathological diagnosis was bronchial glomus tumor. To our knowledge, this presentation (with right upper lobe atelectasis has never before been reported. Glomus tumor should be considered in the differential diagnosis of endobronchial lesions causing lobar atelectasis.

  5. Developing a Tile-Based Rendering Method to Improve Rendering Speed of 3D Geospatial Data with HTML5 and WebGL

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    Seokchan Kang

    2017-01-01

    Full Text Available A dedicated plug-in has been installed to visualize three-dimensional (3D city modeling spatial data in web-based applications. However, plug-in methods are gradually becoming obsolete, owing to their limited performance with respect to installation errors, unsupported cross-browsers, and security vulnerability. Particularly, in 2015, the NPAPI service was terminated in most existing web browsers except Internet Explorer. To overcome these problems, the HTML5/WebGL (next-generation web standard, confirmed in October 2014 technology emerged. In particular, WebGL is able to display 3D spatial data without plug-ins in browsers. In this study, we attempted to identify the requirements and limitations of displaying 3D city modeling spatial data using HTML5/WebGL, and we propose alternative ways based on the bin-packing algorithm that aggregates individual 3D city modeling data including buildings in tile units. The proposed method reduces the operational complexity and the number and volume of transmissions required for rendering processing to improve the speed of 3D data rendering. The proposed method was validated on real data for evaluating its effectiveness in 3D visualization of city modeling data in web-based applications.

  6. Two basic-helix-loop-helix genes (MYC-146 and GL3) from Arabidopsis can activate anthocyanin biosynthesis in a white-flowered Matthiola incana mutant.

    Science.gov (United States)

    Ramsay, Nicola A; Walker, Amanda R; Mooney, Mark; Gray, John C

    2003-06-01

    Basic helix-loop-helix (bHLH) proteins, similar to mammalian Myc transcription factors, regulate the anthocyanin biosynthetic pathway in both monocots and dicots. Two Arabidopsis bHLH genes, GLABRA3 (GL3) and MYC-146, encode proteins that are similar throughout the predicted amino acid sequence to R and DELILA, which regulate anthocyanin production in maize and snapdragon, respectively. Northern blot analysis indicates that MYC-146 is most highly expressed in flower buds and flowers. Expression of a MYC-146 cDNA from the CaMV 35S promoter was unable to complement the anthocyanin deficiency in a ttg1 mutant of Arabidopsis and resulted in no obvious phenotypic change in Columbia plants. However, transient expression of GL3 and MYC-146 upon microprojectile bombardment of petals of a white-flowered mutant of Matthiola incana was able to complement anthocyanin deficiency. The lack of anthocyanin-deficient Arabidopsis mutants mapping to the locations of GL3 and MYC-146 suggests that the two bHLH proteins may be partially redundant and overlap in function.

  7. Inclusão de fibra alimentar em pães isentos de glúten

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    Andressa Luciane Ceccon Saueressig

    Full Text Available Resumo Foram desenvolvidas quatro formulações de pães, isentos de glúten e com a inclusão de fibra alimentar solúvel e insolúvel, avaliando suas propriedades físicas, composição química, aceitação e intenção de compra do consumidor. O padrão (1 teve a farinha de arroz, fécula de mandioca, ovo, leite em pó, óleo de girassol, sal, açúcar, fermento biológico seco e água como ingredientes. Nos demais, a fécula foi parcialmente substituída por inulina (2, farelo de arroz desengordurado e inulina (3, e farelo de arroz desengordurado (4. A cor do pão 2 diferiu apenas na casca, que ficou menos vermelha e amarela que o 1. A inclusão do farelo de arroz proporcionou coloração mais escura, vermelha e amarela à crosta e miolo dos pães 3 e 4. A perda de peso e rendimento variaram apenas em relação ao pão 2, que teve maior perda de peso e menor rendimento, provavelmente devido ao colapso da massa, que não foi capaz de manter a estrutura na fase de crescimento. O custo dos pães com inulina foi maior, pois este ingrediente é um reconhecido prebiótico, adquirido em estabelecimentos farmacêuticos; enquanto que o custo do pão apenas com farelo diminuiu, já que se trata de um subproduto industrial, obtido da extração do óleo de arroz. Na composição química, houve incremento nos teores de cinzas, proteínas e fibra alimentar, principalmente insolúvel, nos pães 3 e 4; enquanto que o pão 2 apresentou menor teor proteico e maior teor de fibra alimentar, principalmente solúvel. Nutricionalmente, admite-se incremento do valor nutricional em detrimento de carboidratos digeríveis e do valor calórico. Sensorialmente, os pães 3 e 4 tiveram menor aceitação nos atributos cor e sabor, mas não diferiram dos pães 1 e 2 nos atributos odor e textura, e na intenção de compra dos consumidores. Conclui-se que o consumo dos pães que tiveram substituição parcial da fécula é garantia de alimento com qualidade funcional

  8. Simultaneous Deletion of the 9GL and UK Genes from the African Swine Fever Virus Georgia 2007 Isolate Offers Increased Safety and Protection against Homologous Challenge.

    Science.gov (United States)

    O'Donnell, Vivian; Risatti, Guillermo R; Holinka, Lauren G; Krug, Peter W; Carlson, Jolene; Velazquez-Salinas, Lauro; Azzinaro, Paul A; Gladue, Douglas P; Borca, Manuel V

    2017-01-01

    African swine fever virus (ASFV) is the etiological agent of a contagious and often lethal viral disease of domestic pigs that has significant economic consequences for the swine industry. The control of African swine fever (ASF) has been hampered by the unavailability of vaccines. Successful experimental vaccines have been derived from naturally occurring, cell culture-adapted, or genetically modified live attenuated ASFV. Recombinant viruses harboring engineered deletions of specific virulence-associated genes induce solid protection against challenge with parental viruses. Deletion of the 9GL (B119L) gene in the highly virulent ASFV isolates Malawi Lil-20/1 (Mal) and Pretoriuskop/96/4 (Δ9GL viruses) resulted in complete protection when challenged with parental isolates. When similar deletions were created within the ASFV Georgia 2007 (ASFV-G) genome, attenuation was achieved but the protective and lethal doses were too similar. To enhance attenuation of ASFV-G, we deleted another gene, UK (DP96R), which was previously shown to be involved in attenuation of the ASFV E70 isolate. Here, we report the construction of a double-gene-deletion recombinant virus, ASFV-G-Δ9GL/ΔUK. When administered intramuscularly (i.m.) to swine, there was no induction of disease, even at high doses (10 6 HAD 50 ). Importantly, animals infected with 10 4 50% hemadsorbing doses (HAD 50 ) of ASFV-G-Δ9GL/ΔUK were protected as early as 14 days postinoculation when challenged with ASFV-G. The presence of protection correlates with the appearance of serum anti-ASFV antibodies, but not with virus-specific circulating ASFV-specific gamma interferon (IFN-γ)-producing cells. ASFV-G-Δ9GL/ΔUK is the first rationally designed experimental ASFV vaccine that protects against the highly virulent ASFV Georgia 2007 isolate as early as 2 weeks postvaccination. Currently, there is no commercially available vaccine against African swine fever. Outbreaks of the disease are devastating to the swine

  9. Dieta isenta de glúten e caseína no transtorno do espectro autista: uma revisão sistemática

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    Ebiene Chaves Dias

    2018-01-01

    Full Text Available Introdução: A dieta sem glúten e sem caseína é uma prática comum no Transtorno do Espectro Autista, mas sem consenso quanto ao seu benefício clínico ou cognitivo. Objetivo: Revisar sistematicamente a literatura que avalia a isenção de glúten e/ou caseína da dieta para indivíduos com Transtorno do Espectro Autista. Materiais e Métodos: Revisão sistemática de literatura analisando estudos originais disponíveis até dezembro/2016 nas bases de dados: PubMed, SciELO, LILACS e BDENF. Os termos usados para a pesquisa foram: autismo, espectro autista, autismo e sem glúten, autismo e dieta livre de caseína. Para melhor direcionamento da busca de dados foi utilizado o método PICO (população, intervenção, comparação e desfecho. Resultados: No total, foram incluídos 22 artigos, sendo 13 ensaios clínicos randomizados, 4 estudos de caso, 4 transversais e 1 coorte. Do total, 15 encontraram uma associação positiva de intervenção para os resultados avaliados e 7 não encontraram associação significativa. Discussão: Foi encontrada grande variabilidade do tamanho amostral, idade, tempo de intervenção, cegamento, controle ou análise dietética mais apurada. Conclusões: não há evidências científicas para apoiar o uso de uma dieta livre de glúten e caseína em pacientes com Transtorno do Espectro Autista. Há necessidade de novos estudos bem delineados, principalmente ensaios clínicos randomizados bem controlados, cegos, com cálculos amostrais que permitam um poder de observação apropriado, para maior segurança nessa prática. Como citar este artigo: Dias EC, Rocha JS, Ferreira GB, Pena GG. Dieta isenta de glúten e caseína no transtorno do espectro autista: uma revisão sistemática. Rev Cuid. 2018; 9(1: 2059-73. http://dx.doi.org/10.15649/cuidarte.v9i1.485

  10. Morfologia e vascularização arterial das glândulas tireóides em gansos domésticos

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    Cheston César Honorato Pereira

    2006-04-01

    Full Text Available Objetivou-se estudar a morfologia e o comportamento das artérias que se destinam às glândulas tireóides, abordando seu número, origem e ordenação em 30 gansos domésticos (Anser domestica, injetados com Neoprene látex 450 corado e fixados em solução aquosa de formalina a 10%. As glândulas tireóides apresentam-se pares, ovóides e localizam-se na extremidade cranial da cavidade tóraco-abdominal, relacionam-se com o nervo vago, veia jugular e artéria carótida comum, e possuem em média de 0,97; 0,69 e 0,43 cm no antímero direito e 1,04; 0,62 e 0,38 cm no antímero esquerdo, para comprimento, largura e espessura, respectivamente. As glândulas tireóides recebem colaterais das artérias: carótida comum, cervical cutânea ascendente, esofagotraqueobronquial, esofágica ascendente, comum do nervo vago ipsilateralmente e ramo esofágico, sendo estes dois últimos somente para a glândula direita. O número de vasos variou de 1 a 5 vasos, sendo 2 vasos (15 casos, 50% ± 10 para o antímero direito e 3 vasos (12 casos, 40% ± 9,8 para o antímero esquerdo, o padrão mais freqüentemente observado. As artérias tireóideas cranial, média cranial, média, média caudal e caudal estiveram presentes no antímero direito em 29, 8, 8, 14 e 29 gansos respectivamente, e no antímero esquerdo em 28, 5, 14, 5 e 28 gansos respectivamente. Um único ramo, a artéria tireóidea, destinou-se a glândula em um animal à direita e em dois animais a esquerda.

  11. Estudo da adição de vital glúten à farinha de arroz, farinha de aveia e amido de trigo na qualidade de pães

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    TEDRUS Guilherme de A. S.

    2001-01-01

    Full Text Available Farinhas de arroz e de aveia e amido de trigo foram adicionados de vital glúten e avaliados pelo Teste de Panificação. Usou-se como padrão uma amostra de farinha de trigo com características adequadas para produção de pão. Os níveis de adição de vital glúten foram de 10, 15, 17,5, 20 e 30% para a farinha de arroz e de 10, 20 e 30% para a farinha de aveia. Ao amido de trigo, extraído da farinha de trigo utilizada como padrão, foi adicionado vital glúten de forma a reconstituir o teor de glúten original da farinha. Os melhores resultados do teste de panificação foram obtidos com 17,5% de vital glúten na farinha de arroz e 20% de vital glúten na farinha de aveia. Foram obtidos pães com volumes específicos inferiores aos de trigo (2,67cm³/g para vital glúten + farinha de arroz, 3,07cm³/g para vital glúten + farinha de aveia e 4,14cm³/g para vital glúten + amido de trigo contra 5,15cm³/g para os de farinha de trigo. As melhores misturas foram analisadas quanto às suas características reológicas e comparadas com a farinha de trigo original. A mistura de amido de trigo e vital glúten apresentou características farinográficas extensográficas inferiores à farinha de trigo e deu origem a pães de menor volume e com estrutura compacta. No geral, a adição de vital glúten propiciou a obtenção de pães com características aceitáveis, embora inferiores às do pão de farinha de trigo. As características dos pães contendo vital glúten foram: pouca simetria e quebra praticamente inexistente. São necessários novos estudos sobre a interação do vital glúten com os demais componentes da farinha de trigo e sua influência na qualidade do produto final.

  12. Tumors of the Major Salivary Glands. A Case Series Study Tumores de las glándulas salivales mayores. Estudio de una serie de casos

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    Teresita Barrios

    2012-11-01

    Full Text Available Background: the study of salivary gland neoplasms is one of the most complex issues of cancer of the head and neck. Objective: to determine the behavior of tumors of the major salivary glands. Method: an observational-descriptive-retrospective case series in the Maxillofacial Surgery Department of the Hospital General Universitario Dr. Gustavo Lima Aldereguía of Cienfuegos, in the period from January 2000 through December 2009. Results: tumors of the major salivary glands occurred in the study population with a similarity between the sexes, with a slight predominance in males. The parotid gland was the most affected (93,55 %, pleomorphic adenoma was the most represented the benign tumor (58,06 %, while within the most frequent malignant adenocarcinoma (3,23 %. The most common surgical technique was subtotal parotidectomy. The (85,5 % of clinical diagnoses corresponded with histopathological and the type of tumor is found that of the 51 benign lesions (82,26 % are the largest percentage of agreement presented. In the present series were 61 patients (98,39 % alive without disease, only 1 patient (1,61 % had recurrence of their disease. We found no patient died during the study period. Conclusions: salivary gland tumors in the parotid gland predominate and are the most common benign tumors usually occur in adults.Fundamento: el estudio de las neoplasias de las glándulas salivales constituye uno de los problemas más complejos de la enfermedad tumoral de la cabeza y el cuello. Objetivo: determinar el comportamiento de los tumores de las glándulas salivales mayores. Método: se realizó un estudio observacional-descriptivo-retrospectivo de una serie de casos en el Servicio de Cirugía Maxilofacial del Hospital General Universitario Dr. Gustavo Aldereguía Lima de Cienfuegos, en el período comprendido entre enero de 2000 hasta diciembre del 2009. Resultados: los tumores de las glándulas salivales mayores se presentaron en la población estudiada

  13. Adaptação nutricional diante da doença celíaca desencadeada pela intolerância ao glúten

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    Francileuda Batista de Almeida

    2016-01-01

    Full Text Available A intolerância ao glúten que desencadeia a doença celíaca DC tem analises e estudo a mais de dois séculos, glúten proteína presente nas farinhas, o doente celíaco tem como sintomas distúrbios gastrointestinais ente outros problemas de saúde decorrente de dietas a parti de alimentos que contem glúten. A partir de tais pressupostos, pretendeu-se com o trabalho de revisão, analisar a necessidade de adaptações nutricionais mediante a doença celíaca desencadeada pela intolerância ao glúten. Neste sentido, tem-se que os sintomas apesentam de formas típicas e atípicas. Fatores intrínsecos também contribuem para o problema tais com os imunológicos, genéticos e ambientais. Estudos comprovam que para melhor convivência com o problema o paciente celíaco deve obter todas as informações tanto do corpo clínico que lhe assiste quanto dos exames realizados como e o caso da biopsia do intestino. O tratamento se baseia em ingestão de dieta sem glúten, antes era difícil encontrar no mercado mais hoje já existem formulações sem glúten bolos goma xantana e biscoito de farrinha de palmeia real. Este trabalho descreve em formato de revisão bibliográfica este assunto.Nutritional adaptation faced with celiac disease triggered by intolerance to glutenAbstract: The intolerance to gluten that triggers the DC celiac disease has analysis and study more than two centuries, gluten protein found in flour, the patient celiac has the symptoms gastrointestinal being disorders other health problems due to diet the left of foods containing gluten. Based on these assumptions, it was intended with the revision work, examine the need for nutritional adaptations by celiac disease triggered by intolerance to gluten-. In this sense, it has to be apesentam symptoms of typical and atypical forms. Intrinsic factors also contribute to the problem with such immunological, genetic and environmental. Studies show that for better interaction with the patient

  14. Tenascin and fibronectin in pleomorphic adenoma of the salivary gland Tenascina e fibronectina em adenoma pleomórfico de glândula salivar

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    Patrícia Meira Bento

    2006-06-01

    Full Text Available OBJECTIVES: To analyze the expression and distribution pattern of extracellular matrix components in pleomorphic adenomas of the major and minor salivary glands and to compare the morphological findings of these tumors with the immunohistochemical expression, considering the different types of stroma predominating in each case. METHODS AND RESULTS: The expression of tenascin (TN and fibronectin (FN was analyzed in 23 cases of pleomorphic adenomas, 11 major and 12 minor salivary gland tumors, by the streptavidin-biotin method using anti-tenascin and anti-fibronectin antibodies. In addition, the immunohistochemical results were correlated with the morphological findings of the lesions. All cases analyzed were immunoreactive for the antibodies used. Fibronectin showed strong labeling in fibrous and chondroid stroma, while labeling was weak in hyaline and myxoid stroma. Tenascin expression was more intense in fibrous and chondroid stroma and moderate in hyaline and myxoid stroma. CONCLUSIONS: No difference in the expression of these proteins was observed between major and minor salivary gland tumors.OBJETIVO: Analisar a expressão e o padrão de distribuição de componentes da matriz extracelular em adenomas pleomórficos de glândula salivar maior e menor e comparar os achados morfológicos destes tumores com a expressão imuno-histoquímica considerando os diferentes tipos de estromas presentes em cada caso. MÉTODOS E RESULTADOS: A expressão da tenascina (TN e fibronectina (FN foi analisada em 23 casos de adenomas pleomórficos, sendo 11 tumores em glândula salivar maior e 12 em glândula salivar menor, utilizando-se o método da estreptoavidina-biotina para os anticorpos anti-tenascina e anti-fibronectina. Os resultados imuno-histoquimicos foram correlacionados com os achados morfológicos das lesões. Todos os casos foram imunorreativos para a fibronectina mostrando forte expressão nos estromas fibrosos e condróides, embora fraca marca

  15. Generalized zeta function representation of groups and 2-dimensional topological Yang-Mills theory: The example of GL(2, 𝔽q) and PGL(2, 𝔽q)

    Science.gov (United States)

    Roche, Ph.

    2016-03-01

    We recall the relation between zeta function representation of groups and two-dimensional topological Yang-Mills theory through Mednikh formula. We prove various generalisations of Mednikh formulas and define generalization of zeta function representations of groups. We compute some of these functions in the case of the finite group GL(2, 𝔽q) and PGL(2, 𝔽q). We recall the table characters of these groups for any q, compute the Frobenius-Schur indicator of their irreducible representations, and give the explicit structure of their fusion rings.

  16. Tumor de glândula ceruminosa com invasão intracraniana: relato de caso Tumor of ceruminous gland with intracranial invasion: case report

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    MÍRIAM C. M. DE CASTRO

    2000-06-01

    Full Text Available As glândulas ceruminosas são glândulas apócrinas modificadas encontradas na região profunda da derme que reveste a porção cartilaginosa do meato acústico externo. Os tumores originados dessas glândulas são raros. Existem controvérsias em relação ao termo ceruminoma, que nos parece impróprio. Na verdade, esses tumores são atualmente classificados como adenoma, adenocarcinoma, carcinoma adenóide cístico e adenoma pleomórfico. Relatamos o caso de uma mulher de 39 anos, apresentado-se com cefaléia, hipoacusia e zumbidos à direita, além de vertigem e náuseas. A TC de crânio evidenciou tumoração do ouvido médio, invadindo os limites ósseos e cartilaginosos, com extensão intracraniana. Foi submetida a cirurgia, com exérese parcial do tumor, seguida de radioterapia (6000 cG. A histologia mostrou tratar-se de carcinoma adenóide cístico de glândulas ceruminosas. A paciente apresentou no pós-operatório paralisia facial periférica à direita, mantendo os déficits anteriores. Após um ano da cirurgia encontra-se estável clinicamente.Ceruminous glands are modified apocrine glands, confined to the skin lining of the cartilaginous part of the external auditory meatus. Tumors arising from these glands are rare. Controversy exists regarding the term "ceruminoma". Actually this neoplasia should be classified as adenoma, adenocarcinoma, adenoid cystic carcinoma and pleomorphic ceruminous adenoma. We report a 39-year-old woman first seen at Santa Casa of Belo Horizonte, in 1998, presenting with headache, nausea, vertigo, hearing loss and tinitus on the right for the past two years. CT scan showed a tumor eroding cartilaginous and bony limits with intracranial invasion. She was submitted to multidisciplinary treatment with surgery followed by radiotherapy (6000 cG. Histology showed a ceruminous adenoid cystic carcinoma. The patient manifested a right peripherical facial palsy and had no recovery of the previous deficits. After one

  17. Comportamiento del manejo de la provisión de unidades de glóbulos rojos en un hospital público Bogotá, dc. 2011

    OpenAIRE

    Robayo Montañez Ligia

    2015-01-01

    Introducción: Uno de los problemas que afrontan los hospitales públicos de Bogotá es la insuficiencia de unidades de glóbulos rojos- UGR “O”; este trabajo pretendió evaluar a través de indicadores, el comportamiento del manejo de inventarios de sangre en un Hospital público de tercer nivel. Método: Estudio descriptivo restrospectivo, a partir de registros de Red Distrital de Sangre, banco de sangre Hemocentro Distrital y del servicio de transfusión del Hospital. La evaluación de gestión del ...

  18. Analysis of the Effect of Cooling Intensity Under Volume-Surface Hardening on Formation of Hardened Structures in Steel 20GL

    Science.gov (United States)

    Evseev, D. G.; Savrukhin, A. V.; Neklyudov, A. N.

    2018-01-01

    Computer simulation of the kinetics of thermal processes and structural and phase transformations in the wall of a bogie side frame produced from steel 20GL is performed with allowance for the differences in the cooling intensity under volume-surface hardening. The simulation is based on the developed method employing the diagram of decomposition of austenite at different cooling rates. The data obtained are used to make conclusion on the effect of the cooling intensity on propagation of martensite structure over the wall section.

  19. Hiposialia por activación de receptores de cannabinoides en la glándula submaxilar. Revisión

    OpenAIRE

    Fernández Solari, Jose Javier; Ossola, Cesar Angel; Prestifilippo, Juan Pablo; Elverdin, Juan Carlos

    2015-01-01

    El presente trabajo aporta evidencia de la presencia de receptores de cannabinoides en la glándula submaxilar de la rata, cuya expresión se circunscribe a componentes acinares y ductales. A su vez, los resultados expuestos confirman la participación de los receptores de cannabinoides en el control de la secreción salival, y por ende aportan una explicación empírica a la hiposialia observada luego del consumo de marihuana. Fil: Fernández Solari, Jose Javier. Universidad de Buenos Aires. Fac...

  20. Colocación de implantes glúteos y de cadera: una alternativa para mejorar el contorno en hipoplasia glúteo-trocantérea Placement of buttock and hip implants: an alternative for contour improvement in buttock and hips hypoplasia

    Directory of Open Access Journals (Sweden)

    L. Cárdenas-Camarena

    2012-03-01

    Full Text Available La mejoría del contorno glúteo y de caderas en un paciente varón que desea un contorno femenino es difícil de conseguir cuando no es posible disponer de una cantidad de grasa suficiente para inyectar y lograr una mejoría integral. Presentamos la técnica quirúrgica utilizada en un paciente transexual masculino que había tenido dos intentos fallidos de feminizar su contorno de glúteos y caderas. Utilizamos implantes ovales de silicona cohesiva, tanto para la región glútea como para las caderas, inyectando grasa en las áreas de transición entre los dos implantes. Con esta combinación obtuvimos un contorno femenino integral, eliminando por completo el aspecto masculino de la región glúteo-trocantérea. En conclusión, pensamos que la combinación empleada supone una opción novedosa y no descrita hasta el momento para el tratamiento de pacientes transexuales que buscan un contorno glúteo más femenino de forma integral, con resultados predecibles y muy gratificantes, que mejora las deficiencias que los pacientes masculinos tienen en esta zona en comparación con el contorno de los glúteos femeninos.Improvement of the gluteal and hip contour in patients seeking a more feminine shape is difficult to achieve, specially for those who don't have enough fat to transfer from other areas of the body. We describe the surgical technique used to improve hips and buttocks contour in a genetic male transsexual. This patient previously underrwent two unsuccessful surgeries to feminize his hip and buttock contour. Our successful approach involved the use of an oval-shaped cohesive silicone gel implants placed in both, the hip and buttock areas, along with fat injection in the areas between the two implants. A natural, feminine contour was achieved using the combination of buttock and hip implants with lipofilling using patient's own fat. These procedures completely eliminated the male contour previously noted in the gluteal trochanteric region

  1. Epidemiology of vampire bat-transmitted rabies virus in Goiás, central Brazil: re-evaluation based on G-L intergenic region.

    Science.gov (United States)

    Hirano, Shinji; Itou, Takuya; Carvalho, Adolorata Ab; Ito, Fumio H; Sakai, Takeo

    2010-11-08

    Vampire bat related rabies harms both livestock industry and public health sector in central Brazil. The geographical distributions of vampire bat-transmitted rabies virus variants are delimited by mountain chains. These findings were elucidated by analyzing a high conserved nucleoprotein gene. This study aims to elucidate the detailed epidemiological characters of vampire bat-transmitted rabies virus by phylogenetic methods based on 619-nt sequence including unconserved G-L intergenic region. The vampire bat-transmitted rabies virus isolates divided into 8 phylogenetic lineages in the previous nucleoprotein gene analysis were divided into 10 phylogenetic lineages with significant bootstrap values. The distributions of most variants were reconfirmed to be delimited by mountain chains. Furthermore, variants in undulating areas have narrow distributions and are apparently separated by mountain ridges. This study demonstrates that the 619-nt sequence including G-L intergenic region is more useful for a state-level phylogenetic analysis of rabies virus than the partial nucleoprotein gene, and simultaneously that the distribution of vampire bat-transmitted RABV variants tends to be separated not only by mountain chains but also by mountain ridges, thus suggesting that the diversity of vampire bat-transmitted RABV variants was delimited by geographical undulations.

  2. Epidemiology of vampire bat-transmitted rabies virus in Goiás, central Brazil: re-evaluation based on G-L intergenic region

    Directory of Open Access Journals (Sweden)

    Ito Fumio H

    2010-11-01

    Full Text Available Abstract Background Vampire bat related rabies harms both livestock industry and public health sector in central Brazil. The geographical distributions of vampire bat-transmitted rabies virus variants are delimited by mountain chains. These findings were elucidated by analyzing a high conserved nucleoprotein gene. This study aims to elucidate the detailed epidemiological characters of vampire bat-transmitted rabies virus by phylogenetic methods based on 619-nt sequence including unconserved G-L intergenic region. Findings The vampire bat-transmitted rabies virus isolates divided into 8 phylogenetic lineages in the previous nucleoprotein gene analysis were divided into 10 phylogenetic lineages with significant bootstrap values. The distributions of most variants were reconfirmed to be delimited by mountain chains. Furthermore, variants in undulating areas have narrow distributions and are apparently separated by mountain ridges. Conclusions This study demonstrates that the 619-nt sequence including G-L intergenic region is more useful for a state-level phylogenetic analysis of rabies virus than the partial nucleoprotein gene, and simultaneously that the distribution of vampire bat-transmitted RABV variants tends to be separated not only by mountain chains but also by mountain ridges, thus suggesting that the diversity of vampire bat-transmitted RABV variants was delimited by geographical undulations.

  3. Normalization of transfection efficiency using the beta-lactamase gene of the pGL3 luciferase vector in primary anterior pituitary cells.

    Science.gov (United States)

    Kang, Seong W; You, Seungkwon; Wong, Eric A; El Halawani, Mohamed E

    2002-08-01

    The beta-galactosidase reporter gene is commonly used as a control for transfection efficiency in the promoter reporter assay system. While investigating vasoactive intestinal peptide response elements in the promoter of the prolactin gene, we found that primary pituitary cells from turkey hens highly expressed endogenous beta-galactosidase. Therefore, we developed a new protocol for determining transfection efficiency using the beta-lactamase gene, which is present on many expression vectors. Transcript levels of beta-lactamase were measured by RT-PCR after transfection of different amounts of the pGL3-basic and pGL3-control vectors. A high correlation was observed between the amount of plasmid transfected and beta-lactamase mRNA levels. Although no eukaryotic promoter was present, there was apparently leaky expression of the beta-lactamase gene. Expression of beta-lactamase was independent of expression from the simian virus 40 or turkey prolactin promoters cloned upstream of the luciferase gene.

  4. Novel DDR Processing of Corn Stover Achieves High Monomeric Sugar Concentrations from Enzymatic Hydrolysis (230 g/L) and High Ethanol Concentration (10% v/v) During Fermentation

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Xiaowen; Jennings, Ed; Shekiro, Joe; Kuhn, Erik M.; O' Brien, Marykate; Wang, Wei; Schell, Daniel J.; Himmel, Mike; Elander, Richard T.; Tucker, Melvin P.

    2015-04-03

    Distilling and purifying ethanol, butanol, and other products from second and later generation lignocellulosic biorefineries adds significant capital and operating cost for biofuels production. The energy costs associated with distillation affects plant gate and life cycle analysis costs. Lower titers in fermentation due to lower sugar concentrations from pretreatment increase both energy and production costs. In addition, higher titers decrease the volumes required for enzymatic hydrolysis and fermentation vessels. Therefore, increasing biofuels titers has been a research focus in renewable biofuels production for several decades. In this work, we achieved over 200 g/L of monomeric sugars after high solids enzymatic hydrolysis using the novel deacetylation and disc refining (DDR) process on corn stover. The high sugar concentrations and low chemical inhibitor concentrations from the DDR process allowed ethanol titers as high as 82 g/L in 22 hours, which translates into approximately 10 vol% ethanol. To our knowledge, this is the first time that 10 vol% ethanol in fermentation derived from corn stover without any sugar concentration or purification steps has been reported. Techno-economic analysis shows the higher titer ethanol achieved from the DDR process could significantly reduce the minimum ethanol selling price from cellulosic biomass.

  5. Adição de colágeno em pão sem glúten elaborado com farinha de arroz

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    Carolina da Silva Graça

    2017-02-01

    Full Text Available Resumo Os portadores da doença celíaca, uma enteropatia, apresentam intolerância ao glúten e a restrição de alimentos que o contenham mostra-se eficaz na normalização da função intestinal e das manifestações clínicas. O objetivo do presente estudo foi avaliar o efeito da adição de colágeno em pó e em fibra nas características estruturais, tecnológicas e sensoriais de pães sem glúten, elaborados com farinha de arroz. Primeiramente, foram realizadas adições de 2%, 4%, 6% e 8% de colágeno, sob as formas em pó e em fibra, em pães elaborados com farinha de arroz, sendo estes avaliados quanto às suas características tecnológicas: volume específico, firmeza do miolo e cor da crosta e do miolo. O pão que apresentou as melhores características tecnológicas foi caracterizado quanto à sua composição química (proteínas, lipídios, cinzas, umidade e carboidratos, avaliado quanto às perdas decorrentes da cocção e submetido à análise sensorial. As massas cruas dos pães adicionados com o colágeno em fibra, assim como a massa do pão padrão (sem adição de colágeno, foram avaliadas através de reologia e microscopia eletrônica de varredura. A adição de colágeno aos pães elaborados com farinha de arroz não colaborou para a formação de uma rede semelhante à formada pelo glúten. O pão da formulação com 4% de colágeno em fibra apresentou as características tecnológicas mais próximas ao esperado, não diferindo significativamente quanto à composição química, do pão padrão. Por meio da análise sensorial, constatou-se que os pães sem glúten (padrão e com 4% de colágeno em fibra obtiveram aceitação sensorial superior a 75%. Conclui-se, com o estudo, que é possível adicionar colágeno em pães sem glúten, sendo que o pão adicionado de 4% de colágeno em fibra apresentou características mais próximas às do pão padrão testado.

  6. Transplante de glândulas salivares labiais no tratamento do olho seco grave Transplantation of labial salivary glands for severe dry eye treatment

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    Eduardo Jorge Carneiro Soares

    2005-08-01

    Full Text Available OBJETIVO: Estudar os efeitos clínicos da secreção das glândulas labiais como alternativa de lubrificação ocular para alívio do olho seco, avaliar a durabilidade dos resultados e simplificar a técnica. MÉTODOS: Estudo prospectivo de 37 cirurgias de enxerto glândulo-mucoso, composto pela mucosa labial e glândulas salivares subjacentes, colocado no fórnice conjuntival de 21 pacientes. Todas as cirurgias foram realizadas em olhos com grave xeroftalmia, sendo a técnica do procedimento descrita em todos os detalhes. Os parâmetros que serviram para a análise comparativa dos resultados foram o quadro clínico, o brilho ocular, o quadro biomicroscópico, a visão e o uso de colírios lubrificantes. RESULTADOS: A integração do enxerto ocorreu em 97,2% das cirurgias que foram realizadas durante o período de julho/2000 a janeiro/2004. Foi observado que em 91,9 % dos casos houve melhora dos parâmetros avaliados e a evolução (acompanhamento médio de 19,7 meses mostrou persistência e estabilidade dos resultados. As complicações observadas foram: um olho com infecção hospitalar e três pálpebras com ptose. CONCLUSÃO: A melhora clínica do olho seco grave observada após o enxerto das glândulas salivares labiais foi estatisticamente significativa. A lubrificação da superfície ocular produzida pela secreção salivar mostrou ser eficiente, bem tolerada e constante. O transplante das glândulas salivares labiais para o fórnice conjuntival demonstrou ser procedimento de fácil execução, acessível a qualquer cirurgião oftalmologista.PURPOSE: To study the clinical effects of the secretion of transplanted labial glands used as ocular lubricant to treat severe dry-eye cases, to evaluate the duration of the results and to simplify the surgical technique. METHODS: Thirty-seven surgeries were performed in twenty-one patients during the period of July 2000 to January 2004. The graft, consisting of labial mucosa and underlying salivary

  7. Umbelliprenin is Potentially Toxic Against the HT29, CT26, MCF-7, 4T1, A172, and GL26 Cell Lines, Potentially Harmful Against Bone Marrow-Derived Stem Cells, and Non-Toxic Against Peripheral Blood Mononuclear Cells.

    Science.gov (United States)

    Rashidi, Mohsen; Ziai, Seyed Ali; Moini Zanjani, Taraneh; Khalilnezhad, Ahad; Jamshidi, Hamidreza; Amani, Davar

    2016-07-01

    Resistance to chemotherapy is a growing concern, thus natural anticancer agents are drawing the attention of many scientists and clinicians. One natural anticancer agent, umbelliprenin, is a coumarin produced by many species of Ferula. We aimed to examine the inhibitory effect of umbelliprenin on human and mouse bone marrow-derived stem cells (BMDSCs), peripheral blood mononuclear cells (PBMCs), and different cancer cell lines. In this in vitro experimental study, the HT29, CT26, MCF-7, 4T1, A172, and GL26 cancer cells and human and mouse BMDSCs and PBMCs were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), incubated at 37°C for 24 hours in a 5% CO2 atmosphere, and then were treated with different concentrations of umbelliprenin dissolved in dimethyl sulfoxide (DMSO) (3, 6, 12, 25, 50, 100, and 200 µg/mL) for 24, 48, and 72 hours at 37°C. Each experiment was performed in triplicate. Finally, the cell survival rate was assessed by MTT assay. The IC50 values were calculated based on the log values using GraphPad Prism version 5 software for windows (La Jolla CA, USA) and were expressed as mean ± SEM. Umbelliprenin inhibited the cancer cells in a concentration-dependent (P 0.05). The most sensitive and resistant cell lines at the 24-hour incubation time were 4T1 (IC50, 30.9 ± 3.1 µg/mL) and A172 (IC50, 51.9 ± 6.7 µg/mL); at the 48-hour incubation time: 4T1 (IC50, 30.6 ± 2.6 µg/mL) and CT26 (IC50, 53.2 ± 3.6 µg/mL); and at the 72-hour incubation time: HT29 (IC50, 37.1 ± 1.4 µg/mL) and 4T1 (IC50, 62.2 ± 4.8 µg/mL). Both human and mouse BMDSCs showed the highest resistance at the 24-hour incubation time (IC50s, 254.7 ± 21 and 204.4 ± 4.5 µg/mL, respectively) and the highest sensitivity at the 72-hour incubation time (IC50s, 120.4 ± 5 and 159.0 ± 7.3 µg/mL, respectively). The PBMCs of both human and mouse origin revealed very strong resistance to the studied concentrations of umbelliprenin (IC50s ranging from 713.5 ± 499

  8. A expressão da COX-2 em patologias da glândula mamária da gata

    OpenAIRE

    Sousa, Rui Pedro Meireles de

    2010-01-01

    Pelo menos 80-85% dos tumores da glândula mamária dos felídeos são malignos. Estes tumores ocorrem em média entre os 10-11 anos de idade e são o terceiro tumor mais frequente nesta espécie, precedidos dos tumores hematopoiéticos e cutâneos. A incidência destes tumores nos felídeos é inferior em cerca de metade aos dos canídeos e humanos. Contudo, as neoplasias mamárias correspondem a 17% de todos os tumores que ocorrem na gata, que metastizam com frequência, principalmente para o pulmão e gân...

  9. Hypoglycaemic and hypolipidaemic effects of low GI and medium GL Indian diets in type 2 diabetics for a period of 4 weeks: a prospective study.

    Science.gov (United States)

    Pande, Ashwini; Krishnamoorthy, Geetha; Moulick, N D

    2012-09-01

    This prospective study reports significant hypoglycaemic and hypolipidaemic effects in type 2 diabetic subjects who were provided the complete diet plan to be on low glycaemic index (GI) and low-medium glycaemic load (GL) Indian vegetarian snacks and mixed meals for 4 continuous weeks. Five millilitres of fasting blood sample drawn at weekly intervals for 4 weeks were analysed for blood glucose, HbA1c and lipid profile. Four weeks later mean blood glucose level of 173.6 mg% decreased to 137.8 mg%, HbA1c of 8% also decreased to 7.1% which reflected the blood glucose level during the study period and hence correlated well with the fall in blood glucose level. Triglyceride level of 244.5 mg% decreased to 164.7 mg% (p stress.

  10. Baxterization of GL{sub q}(2) and its application to the Liouville model and some other model on a lattice

    Energy Technology Data Exchange (ETDEWEB)

    Bytsko, A.G. [Steklov Mathematics Institute, St. Petersburg (Russian Federation)]|[Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)

    2007-12-15

    We develop the Baxterization approach to (an extension of) the quantum group GL{sub q}(2). We introduce two matrices which play the role of spectral parameter dependent L-matrices and observe that they are naturally related to two different comultiplications. Using these comultiplication structures, we find the related fundamental R-operators in terms of powers of coproducts and also give their equivalent forms in terms of quantum dilogarithms. The corresponding quantum local Hamiltonians are given in terms of logarithms of positive operators. An analogous construction is developed for the q-oscillator and Weyl algebras using that their algebraic and coalgebraic structures can be obtained as reductions of those for the quantum group. As an application, the lattice Liouville model, the q-DST model, the Volterra model, a lattice regularization of the free field, and the relativistic Toda model are considered. (orig.)

  11. Combined molecular MRI and immuno-spin-trapping for in vivo detection of free radicals in orthotopic mouse GL261 gliomas.

    Science.gov (United States)

    Towner, Rheal A; Smith, Nataliya; Saunders, Debra; De Souza, Patricia Coutinho; Henry, Leah; Lupu, Florea; Silasi-Mansat, Robert; Ehrenshaft, Marilyn; Mason, Ronald P; Gomez-Mejiba, Sandra E; Ramirez, Dario C

    2013-12-01

    Free radicals play a major role in gliomas. By combining immuno-spin-trapping (IST) and molecular magnetic resonance imaging (mMRI), in vivo levels of free radicals were detected within mice bearing orthotopic GL261 gliomas. The nitrone spin trap DMPO (5,5-dimethyl pyrroline N-oxide) was administered prior to injection of an anti-DMPO probe (anti-DMPO antibody covalently bound to a bovine serum albumin (BSA)-Gd (gadolinium)-DTPA (diethylene triamine penta acetic acid)-biotin MRI contrast agent) to trap tumor-associated free radicals. mMRI detected the presence of anti-DMPO adducts by either a significant sustained increase (pfree radicals from a glioma model. © 2013.

  12. Morphology of the male agouti accessory genital glands (Dasyprocta prymnolopha Wagler, 1831 Morfologia das glândulas genitais acessórias em cutias (Dasyprocta prymnolopha Wagler, 1831

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    Danilo J. Ayres de Menezes

    2010-09-01

    Full Text Available The morphology of the accessory genital glands of the male agouti was studied in twenty-three animals that were raised in captivity. Twenty animals had their genital glands dissected in situ for macroscopic description. The samples of each gland were recovered, embedded in paraffin, sliced and stained by Hematoxylin-Eosin technique. It was founded four pairs of glands: the vesicular glands, the coagulating glands, the prostate and the bulbourethral glands. Histological characteristics of the vesicular, coagulating and prostate glands showed similar morphology, within the pseudostratified columnar epithelium. The tubulo-alveolar type of the bulbourethral glands showed a lack of connective tissue among the tubules, a small amount of red stained presented it the cytoplasm, and the presence of vacuoles in the tissue. This study concluded that the agouti showed to have similar morphological aspect described in the others species of rodents.A morfologia das glândulas genitais acessórias de cutias foram estudados em 23 animais criados em cativeiros. Vinte animais tiveram suas glândulas genitais dissecadas in situ para as descrições macroscópicas. Para o estudo microscópico foram utilizados três animais. Os fragmentos de cada glândula foram embebidos em parafina, seccionados e corados em hematoxilina e eosina. Foram encontrados quatro pares de glândulas: vesiculares, coaguladoras, próstata e bulbouretrais. As características histológicas da glândula vesicular, coaguladora e próstata mostraram morfologia similar, com epitélio colunar pseudoestratificado. O tipo tuboalveolar da glândula bulbouretral mostrou uma deficiência de tecido conjuntivo, citoplasma pouco corado e presença de vacúolos. Este estudo concluiu que a cutia apresenta as mesmas características morfológicas das glândulas genitais acessórias encontradas em roedores.

  13. African swine fever virus Georgia isolate harboring deletions of 9GL and MGF360/505 genes is highly attenuated in swine but does not confer protection against parental virus challenge.

    Science.gov (United States)

    O'Donnell, Vivian; Holinka, Lauren G; Sanford, Brenton; Krug, Peter W; Carlson, Jolene; Pacheco, Juan M; Reese, Bo; Risatti, Guillermo R; Gladue, Douglas P; Borca, Manuel V

    2016-08-02

    African swine fever virus (ASFV) produces a contagious disease of domestic pigs that results in severe economic consequences to the swine industry. Control of the disease has been hampered by the unavailability of vaccines. We recently reported the development of two experimental vaccine strains (ASFV-G-Δ9GL and ASFV-G-ΔMGF) based on the attenuation of the highly virulent and epidemiologically relevant Georgia2007 isolate. Deletion of the 9GL gene or six genes of the MGF360/505 group produced two attenuated ASFV strains which were able to confer protection to animals when challenged with the virulent parental virus. Both viruses, although efficient in inducing protection, present concerns regarding their safety. In an attempt to solve this problem we developed a novel virus strain, ASFV-G-Δ9GL/ΔMGF, based on the deletion of all genes deleted in ASFV-G-Δ9GL and ASFV-G-ΔMGF. ASFV-G-Δ9GL/ΔMGF is the first derivative of a highly virulent ASFV field strain subjected to a double round of recombination events seeking to sequentially delete specific genes. ASFV-G-Δ9GL/ΔMGF showed a decreased ability to replicate in primary swine macrophage cultures relative to that of ASFV-G and ASFV-G-ΔMGF but similar to that of ASFV-G-Δ9GL. ASFV-G-Δ9GL/ΔMGF was attenuated when intramuscularly inoculated into swine, even at doses as high as 10(6) HAD50. Animals infected with doses ranging from 10(2) to 10(6) HAD50 did not present detectable levels of virus in blood at any time post-infection and they did not develop detectable levels of anti-ASFV antibodies. Importantly, ASFV-G-Δ9GL/ΔMGF does not induce protection against challenge with the virulent parental ASFV-G isolate. Results presented here suggest caution towards approaches involving genomic manipulations when developing rationally designed ASFV vaccine strains. Published by Elsevier B.V.

  14. Reactive Oxygen Species-Dependent Apoptosis by Gugulipid Extract of Ayurvedic Medicine Plant Commiphora mukul in Human Prostate Cancer Cells Is Regulated by c-Jun N-Terminal KinaseS⃞

    Science.gov (United States)

    Zeng, Yan; Prakash, Lakshmi; Badmaev, Vladmir; Majeed, Muhammed; Singh, Shivendra V.

    2011-01-01

    Gugulipid (GL), extract of Indian Ayurvedic medicinal plant Commiphora mukul, has been used to treat a variety of ailments. We report an anticancer effect and mechanism of GL against human prostate cancer cells. Treatment with GL significantly inhibited the viability of human prostate cancer cell line LNCaP (androgen-dependent) and its androgen-independent variant (C81) with an IC50 of ∼1 μM (24-h treatment), at pharmacologically relevant concentrations standardized to its major active constituent z-guggulsterone. The GL-induced growth inhibition correlated with apoptosis induction as evidenced by an increase in cytoplasmic histone-associated DNA fragmentation and sub-G0/G1-DNA fraction, and cleavage of poly(ADP-ribose) polymerase. The GL-induced apoptosis was associated with reactive oxygen species (ROS) production and c-Jun NH2-terminal kinase (JNK) activation. The induction of proapoptotic Bcl-2 family proteins Bax and Bak and a decrease of antiapoptotic Bcl-2 protein Bcl-2 were observed in GL-treated cells. SV40 immortalized mouse embryonic fibroblasts derived from Bax-Bak double-knockout mice were significantly more resistant to GL-induced cell killing compared with wild-type cells. It is interesting to note that a representative normal prostate epithelial cell line (PrEC) was relatively more resistant to GL-mediated cellular responses compared with prostate cancer cells. The GL treatment caused the activation of JNK that functioned upstream of Bax activation in apoptosis response. The GL-induced conformational change of Bax and apoptosis were significantly suppressed by genetic suppression of JNK activation. In conclusion, the present study indicates that ROS-dependent apoptosis by GL is regulated by JNK signaling axis. PMID:21115635

  15. Reactive oxygen species-dependent apoptosis by gugulipid extract of Ayurvedic medicine plant Commiphora mukul in human prostate cancer cells is regulated by c-Jun N-terminal kinase.

    Science.gov (United States)

    Xiao, Dong; Zeng, Yan; Prakash, Lakshmi; Badmaev, Vladmir; Majeed, Muhammed; Singh, Shivendra V

    2011-03-01

    Gugulipid (GL), extract of Indian Ayurvedic medicinal plant Commiphora mukul, has been used to treat a variety of ailments. We report an anticancer effect and mechanism of GL against human prostate cancer cells. Treatment with GL significantly inhibited the viability of human prostate cancer cell line LNCaP (androgen-dependent) and its androgen-independent variant (C81) with an IC(50) of ∼1 μM (24-h treatment), at pharmacologically relevant concentrations standardized to its major active constituent z-guggulsterone. The GL-induced growth inhibition correlated with apoptosis induction as evidenced by an increase in cytoplasmic histone-associated DNA fragmentation and sub-G(0)/G(1)-DNA fraction, and cleavage of poly(ADP-ribose) polymerase. The GL-induced apoptosis was associated with reactive oxygen species (ROS) production and c-Jun NH(2)-terminal kinase (JNK) activation. The induction of proapoptotic Bcl-2 family proteins Bax and Bak and a decrease of antiapoptotic Bcl-2 protein Bcl-2 were observed in GL-treated cells. SV40 immortalized mouse embryonic fibroblasts derived from Bax-Bak double-knockout mice were significantly more resistant to GL-induced cell killing compared with wild-type cells. It is interesting to note that a representative normal prostate epithelial cell line (PrEC) was relatively more resistant to GL-mediated cellular responses compared with prostate cancer cells. The GL treatment caused the activation of JNK that functioned upstream of Bax activation in apoptosis response. The GL-induced conformational change of Bax and apoptosis were significantly suppressed by genetic suppression of JNK activation. In conclusion, the present study indicates that ROS-dependent apoptosis by GL is regulated by JNK signaling axis.

  16. Há diferença na atividade do músculo glúteo médio em diferentes posições de quadril durante o exercício inclinação da pelve?

    OpenAIRE

    Renan Lima Monteiro

    2015-01-01

    Exercícios de inclinação da pelve (\\"pelvic drop\\") são frequentemente utilizados para o fortalecimento do músculo glúteo médio com o objetivo de aumentar ou priorizar o seu recrutamento. No entanto, o efeito da rotação do quadril no desempenho da ação do glúteo médio é desconhecido. O objetivo do estudo foi avaliar o efeito da rotação do quadril no recrutamento do músculo glúteo médio, tensor da fascia lata e quadrado lombar. Dezessete sujeitos saudáveis executaram o exercício de inclinação ...

  17. Addition of CaCO3 to culture media at the salinity of 3 g/L for freshwater tambaqui growth

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    Yuni Puji Hastuti

    2016-02-01

    Full Text Available ABSTRACT Increasing of freshwater tambaqui Colossoma macropomum demand makes the farmers increase the production of the consumption fish and seed. Acceleration of the production cycle can increase total production level, and reduce the level of osmotic work can be used to improve fish growth. This study aimed to analyze the effect of the addition of calcium carbonate (CaCO3 in the saline medium of 3 g/L on the growth of freshwater tambaqui juvenile. Tambaqui used has a body length of 1.93 ± 0.1 cm and weight of 0.26 ± 0.03 g. The experiment used 15 units of aquarium at size of 30×15×25 cm3 and filled with 9 L of saline water, then added lime CaCO3 according to treatment. The treatments were control (0 mg/L CaCO3, A (50 mg/L CaCO3, B (100 mg/L CaCO3, C (150 mg/L CaCO3, and D (200 mg/L CaCO3. The study was conducted for 30 days of maintenance. Fishes were fed on bloodworms ad libitum or provided three times a day. The results showed that survival, daily growth rate, and absolute length growth of the CaCO3 treatments significantly higher (P<0.05 that that of control.  Furthermore, survival, daily growth rate, and absolute length growth among the CaCO3 treatments were the same.  Thus, addition CaCO3 of 50 mg/L saline water of 3 g/L can be applied to increase culture performance of freshwater tambaqui. Keywords: freshwater tambaqui, CaCO3, salinity  ABSTRAK Permintaan terhadap ikan bawal air tawar Colossoma macropomum yang semakin meningkat membuat pembudidaya menambah produksi ikan konsumsi dan benih. Percepatan siklus produksi dapat meningkatkan total produksi budidaya, dan energi dari optimasi kerja osmotik dapat dialokasikan untuk pertumbuhan. Penelitian ini bertujuan untuk menganalisis pengaruh penambahan kalsium karbonat (CaCO3 pada media bersalinitas terhadap pertumbuhan benih ikan bawal air tawar. Benih ikan bawal yang digunakan memiliki panjang 1,93±0,1 cm dengan bobot 0,26±0,03 g. Akuarium yang digunakan berukuran 30×15×25 cm

  18. Resultados oncológicos da laringectomia parcial no carcinoma glótico inicial Oncologic results of the partial laryngectomy for early glottic carcinoma

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    Otávio Alberto Curioni

    2002-10-01

    Full Text Available Introdução: O carcinoma epidermóide da laringe ocupa o sexto lugar entre as neoplasias mais comuns, sendo uma das malignidades mais freqüentes na cabeça e pescoço, ocupando o segundo lugar imediatamente após o câncer da cavidade oral. Dependendo do estadiamento da lesão, ou seja, extensão do câncer ao diagnóstico, há um prognóstico a longo prazo bastante favorável, com taxas de sobrevida global para 5 anos de 65%-70%. Objetivo: Avaliação dos resultados após laringectomia parcial para carcinoma glótico inicial. Forma de estudo: Clínico retrospectivo. Material e Método: Foi realizada análise retrospectiva do prontuário de 59 pacientes submetidos à laringectomia parcial. Foram excluídos pacientes com tratamento prévio, extensão tumoral maior que 10 mm abaixo da glote, lesões com extensão macroscópica para a(s prega(s vestibular(s e lesões com diminuição grosseira da mobilidade da(s prega(a vocal(is à laringoscopia indireta e aqueles com seguimento inferior a 36 meses. Resultados: Doze (20,3% dos 59 pacientes apresentaram recidiva, sendo uma recidiva regional (1,7% e 11 recidivas locais (18,6%. Com o resgate cirúrgico (laringectomia total, controle oncológico foi alcançado em 75% desses casos. Complicações severas (estenose glótica e aspiração traqueobrônquica ocorreram em somente 2 pacientes (3,4%. Conclusões: As laringectomias parciais são procedimentos seguros e eficazes para o tratamento da maioria dos casos de câncer glótico inicial, além do que, pela sua versatilidade, conferem segurança na radicalidade oncológica e possibilitam, também, a readaptação funcional ao paciente.Introduction: The squamous cell carcinoma of the larynx is the sixth most common neoplasia, being the the second neoplasia of the head and neck, immediately after oral cancer. Depending of the stage of lesion, extension of the cancer at diagnosis, there is a quite favorable long term prognosis, with global survival rate

  19. Estudio morfométrico sobre el desarrollo y evolución de las glándulas tiroides durante la metamorfosis de Bufo arenarum

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    Miranda, Leandro Andrés

    1996-01-01

    Full Text Available Se ha estudiado el desarrollo y la evolución de las glándulas tiroideas durante la morfogénesis de Bufo arenarum. Usando diferentes parámetros morfométricos, hemos relacionado el crecimiento y desarrollo larval con el desarrollo de las glándulas tiroideas. Se observó que la longitud total, el peso de las larvas, el volumen glandular tiroideo y el diámetro folicular, aumentan hasta el estadio XV (fin de la prometamorfosis, mientras que el número de folículos y la altura de las células foliculares tiroideas se incrementan hasta el estadio XVII (mitad del clímax metamórfico. Todos estos parámetros disminuyeron considerablemente durante el clímax metamórfico mientras que en animales postmetamórficos se observó un incremento en los valores registrados. En base a los resultados obtenidos concluimos que hay un período de síntesis y almacenamiento de hormonas tiroideas durante el crecimiento larval y un segundo período caracterizado por la liberación de hormonas tiroideas hacia el final de la prometamorfosis y durante el clímax metamórfico. We studied the development and evolution of thyroid glands during Bufo arenarum morphogenesis. Using different morphometric parameters we related larval growth and metamorphosis with thyroid glands development We observed that total length, larval weight, thyroid gland volume and follicle diameter increased until stage XV (end of prometamorphosis, meanwhile the number of follicles and follicle cell height increased until stage XVII (midclimax. All these parameters decreased during metamorphic climax and an increase was observed in postmetamorphic animals. Our results lead us to conclude that during larval growth there is a period of synthesis and store of thyroid hormones. There is a second period characterized by thyroid hormones release during the end of prometamorphosis and metamorphic climax.

  20. African Swine Fever Virus Georgia 2007 with a Deletion of Virulence-Associated Gene 9GL (B119L), when Administered at Low Doses, Leads to Virus Attenuation in Swine and Induces an Effective Protection against Homologous Challenge.

    Science.gov (United States)

    O'Donnell, Vivian; Holinka, Lauren G; Krug, Peter W; Gladue, Douglas P; Carlson, Jolene; Sanford, Brenton; Alfano, Marialexia; Kramer, Edward; Lu, Zhiqiang; Arzt, Jonathan; Reese, Bo; Carrillo, Consuelo; Risatti, Guillermo R; Borca, Manuel V

    2015-08-01

    African swine fever virus (ASFV) is the etiological agent of an often lethal disease of domestic pigs. Disease control strategies have been hampered by the unavailability of vaccines against ASFV. Since its introduction in the Republic of Georgia, a highly virulent virus, ASFV Georgia 2007 (ASFV-G), has caused an epizootic that spread rapidly into Eastern European countries. Currently no vaccines are available or under development to control ASFV-G. In the past, genetically modified ASFVs harboring deletions of virulence-associated genes have proven attenuated in swine, inducing protective immunity against challenge with homologous parental viruses. Deletion of the gene 9GL (open reading frame [ORF] B119L) in highly virulent ASFV Malawi-Lil-20/1 produced an attenuated phenotype even when administered to pigs at 10(6) 50% hemadsorption doses (HAD50). Here we report the construction of a genetically modified ASFV-G strain (ASFV-G-Δ9GLv) harboring a deletion of the 9GL (B119L) gene. Like Malawi-Lil-20/1-Δ9GL, ASFV-G-Δ9GL showed limited replication in primary swine macrophages. However, intramuscular inoculation of swine with 10(4) HAD50 of ASFV-G-Δ9GL produced a virulent phenotype that, unlike Malawi-Lil-20/1-Δ9GL, induced a lethal disease in swine like parental ASFV-G. Interestingly, lower doses (10(2) to 10(3) HAD50) of ASFV-G-Δ9GL did not induce a virulent phenotype in swine and when challenged protected pigs against disease. A dose of 10(2) HAD50 of ASFV-G-Δ9GLv conferred partial protection when pigs were challenged at either 21 or 28 days postinfection (dpi). An ASFV-G-Δ9GL HAD50 of 10(3) conferred partial and complete protection at 21 and 28 dpi, respectively. The information provided here adds to our recent report on the first attempts toward experimental vaccines against ASFV-G. The main problem for controlling ASF is the lack of vaccines. Studies on ASFV virulence lead to the production of genetically modified attenuated viruses that induce protection

  1. Estudos histológicos sôbre as glândulas peçonhentas da "Viúva negra", Latrodectus mactans (Fabricius (Arachnida, Araneae, Theridiidae

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    Rudolf Barth

    1962-07-01

    Full Text Available A glândula peçonhenta de Latrodectus mactans compõe-se: 1. De um saco formado por uma membrana de tecido conjuntivo peritonial, que se projeta proximalmente no ducto de peçonha. 2. De um manto externo, formado por 40-45 feixes musculares, circundando 3/4 da glândula, helicoidalmente. Os feixes inserem-se nas extremidades apical e basal da membrana sarcoperitonial; sua contração impele a peçonha, através do ducto, para as garras. 3. Do epitélio glandular, composto: a de células da glândula principal, b de células da glândula auxiliar, c de célula da glândula situada à entrada do ducto. As células das glândulas principal e auxiliar, juntas, formam uma unidade glandular de natureza ragiócrina. A secreção das células da glândula auxiliar passa, através das membranas celulares, para o interior das células glandulares principais, deslocando-se, juntamente com a secreção destas, para o pólo apical, a que ambas confluem, formando uma substância viscosa, fortemente condensada em virtude da eliminação de certos líquidos. Após a formação de 8 a 10 destas porções de secreções, os dois tipos de células esgotam-se; não se dá a regeneração ou substituição por outras células. A quantidade de peçonha, uma vez produzida, é armazenada na cavidade da glândula, sendo suficiente para tôda a vida do animal. As células da glândula situada á entrada do ducto, ou glândula lipócrina, produzem uma substãncia lipóide. Esta substância dissolve uma porção de corpúsculos das secreções segregadas pelas células principais e auxiliares. O líquido resultante é inoculado no tecido da prêsa ou do inimigo, por contração do manto muscular. Ao mesmo tempo, novas porções da peçonha armazenada são transportadas no sentido proximal e dissolvidas no líquido lipóide, preparando-se, assim, nova peçonha para outra picada. As formas das células e os pormenores histológicos estão explicados nas figuras que

  2. First comparison of adjuvant for trivalent inactivated Haemophilus parasuis serovars 4, 5 and 12 vaccines against Glässer's disease.

    Science.gov (United States)

    Xue, Qiao; Zhao, Zhanqin; Liu, Huisheng; Chen, Kunpeng; Xue, Yun; Wang, Le

    2015-12-15

    Haemophilus parasuis has had a huge impact in the swine industry throughout the world. Inactivated bacterium for H. parasuis is a traditional vaccine that can elicit efficient protection against homologous challenges. The objective of this study was to screen for the adjuvant-enhanced immune effect of trivalent inactivated H. parasuis serovars 4, 5 and 12 (prevalent serovars in China) vaccines against Glässer's disease. The adjuvants of mineral oil, aluminum hydroxide, Montanide GEL 01 PR, Montanide IMS 1313N VG and Montanide ISA 760 VG were used to make emulsified inactivated H. parasuis serovars 4, 5 and 12, respectively. Safety, antibody titer and protective efficacy of these vaccines were examined separately in piglets, and the feasibility of microagglutination test for detecting antibody titer of H. parasuis was confirmed for the first time. Due to easy of injection, high safety, rapidly immune responses, high concentrations of antibody, and 100% of protective efficacy for piglets, Montanide GEL 01 PR adjuvant can provide more homologous serovar protection than other domestically developed inactivated vaccines and should be used as a candidate adjuvant. Copyright © 2015 Elsevier B.V. All rights reserved.

  3. [Hypertension with chronic kidney disease: anti-hypertensive therapy recommended for the management of hypertension with CKD in JSN-CKD GL 2013 and JSH2014].

    Science.gov (United States)

    Tamura, Kouichi; Yutoh, Jun; Matsushita, Kei; Sakai, Masashi; Oshikawa, Jin

    2015-11-01

    For clinical practice guidelines for the management of hypertension with CKD, the Japanese Society of Nephrology (JSN) and the Japanese Society of Hypertension (JSH) evaluated recently published evidence in corporation with each other. After considerable and careful discussion, both JSN and JSH revised their respective guidelines [the Evidence-based Clinical Practice Guideline for CKD 2013 (JSN-CKD GL 2013) and JSH2014]. This section will mainly introduce anti-hypertensive therapy recommended for the management of hypertension with CKD in both guidelines. Recommendation statements for the Management of Hypertension with CKD are as follows: 1) Anti-hypertensive therapy in CKD is strongly recommended to inhibit or prevent the progression of renal dysfunction and to prevent the occurrence or recurrence of CVD by reducing blood pressure (BP) (Grade A). 2) In all diabetic CKD, the target level of clinic BP is recommended as anti-hypertensive drugs(Grade C1). 6) In diabetic CKD with A2 and A3 categories, ARBs and ACE inhibitors are recommended as first-line anti-hypertensive drugs (Grade A). 7) In non-diabetic CKD with A1 category, ARBs, ACE inhibitors, calcium channel blockers (CCBs) and diuretics are recommended as first-line anti-hypertensive drugs (Grade B). 8) In non-diabetic CKD with A2 and A3 categories, ARBs and ACE inhibitors are recommended as first-line anti-hypertensive drugs (Grade B).

  4. Comparison of IgG IgGl lgG2 immune responses to pneumococcal polysaccharide in atopic nonatopic children

    Directory of Open Access Journals (Sweden)

    Emiko Noguchi

    1998-01-01

    Full Text Available The levels of naturally occurring IgG, IgGl and lgG2 antibodies against polyvalent pneumococcal capsular polysaccharide antigen (Pneumovax® were compared between atopic and nonatopic children with different ages, 6–12 months and 1, 2, 3, 4, 5–9 and 10–15 years, by enzyme-linked immunosorbent assay. Children with asthma, atopic dermatitis, food allergy or a combination of these, and those having serum IgE levels exceeding 50 IU/mL at 6–12 months old and 100 IU/mL at more than 1-year-old were included as atopic groups. Asymptomatic children whose serum IgE levels were less than these atopic standards and those not having detectable IgE antibodies to Dermatophagoides farinae comprised the nonatopic groups. Geometric mean levels of IgG and IgG1 antibodies against pneumococcal antigen increased steadily with age, and that of IgG2 antibodies was low until 3 years of age and then gradually increased age-dependently up to 15 years of age. The levels of IgG antibody as well as IgG1 and IgG2 antibodies were not significantly different between atopic and nonatopic children in any age group. This suggests that the immune response to the most common bacterial pathogen in the respiratory tract does not influence atopic status.

  5. Efeito da hiperprolactinemia induzida pela metoclopramida na glândula lacrimal: estudo experimental Effects of metoclopramide-induced hyperprolactinemia on the lacrimal gland: experimental study

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    Carina Verna

    2005-09-01

    Full Text Available OBJETIVO: avaliar as alterações morfológicas promovidas pela hiperprolactinemia induzida pela metoclopramida na glândula lacrimal de camundongas durante a fase de proestro e gestação. MÉTODOS: 40 camundongas adultas foram divididas em dois grupos: CTR1 (controle e MET1 (tratadas com metoclopramida. Após 50 dias, metade dos animais de cada grupo foram sacrificados por decapitação. Os restantes foram acasalados, constituindo os grupos controle prenhe (CTR2 e metoclopramida prenhe (MET2, que foram sacrificadas no 6º dia de gestação. O sangue foi coletado e submetido à dosagem hormonal dos níveis de estradiol e de progesterona por quimioluminescência. Em seguida as glândulas lacrimais foram removidas, fixadas em formol a 10% e processadas segundo metodologia histológica para inclusão em parafina, sendo as lâminas coradas pelo HE. A análise morfométrica foi realizada com o programa AxionVision (Carl Zeiss, medindo-se os volumes celulares e nucleares das células acinares. RESULTADOS: os volumes nuclear e celular das glândulas lacrimais dos grupos experimentais MET1 (152,2±8,7; 6,3±1,6 µm³ e MET2 (278,3±7,9; 27,5±0,9 µm³ mostraram-se reduzidos em relação aos grupos controles CTR1 (204,2±7,4; 21,9±1,3 µm³ e CTR2 (329,4±2,2; 35,5±2,0 µm³. Houve redução dos níveis hormonais de estrogênio e de progesterona nos animais que receberam metoclopramida em comparação com os respectivos controles (CTR1: estradiol = 156,6±42,2 pg/ml; progesterona = 39,4±5,1 ng/ml; MET1: estradiol = 108,0±33,1 pg/ml; progesterona = 28,0±6,4 ng/ml; CTR2: estradiol = 354,0±56,0 pg/ml; progesterona = 251,0±56,0 ng/ml; MET2: estradiol = 293,0±43,0 pg/ml, progesterona = 184,0±33,0 ng/ml. CONCLUSÃO: a hiperprolactinemia induzida pela metoclopramida produziu sinais de diminuição na atividade das células da glândula lacrimal tanto em camundongas prenhes quanto não prenhes. Este efeito está possivelmente relacionado com a redu

  6. Two-dimensional patterning by a trapping/depletion mechanism: the role of TTG1 and GL3 in Arabidopsis trichome formation.

    Directory of Open Access Journals (Sweden)

    Daniel Bouyer

    2008-06-01

    Full Text Available Trichome patterning in Arabidopsis serves as a model system to study how single cells are selected within a field of initially equivalent cells. Current models explain this pattern by an activator-inhibitor feedback loop. Here, we report that also a newly discovered mechanism is involved by which patterning is governed by the removal of the trichome-promoting factor TRANSPARENT TESTA GLABRA1 (TTG1 from non-trichome cells. We demonstrate by clonal analysis and misexpression studies that Arabidopsis TTG1 can act non-cell-autonomously and by microinjection experiments that TTG1 protein moves between cells. While TTG1 is expressed ubiquitously, TTG1-YFP protein accumulates in trichomes and is depleted in the surrounding cells. TTG1-YFP depletion depends on GLABRA3 (GL3, suggesting that the depletion is governed by a trapping mechanism. To study the potential of the observed trapping/depletion mechanism, we formulated a mathematical model enabling us to evaluate the relevance of each parameter and to identify parameters explaining the paradoxical genetic finding that strong ttg1 alleles are glabrous, while weak alleles exhibit trichome clusters.

  7. Arquitetura e moda - ina Bo Bardi e Glória Coelho: possíveis relações entre as áreas e as profissionais

    Directory of Open Access Journals (Sweden)

    Natana Aparecida de Oliveira

    2015-12-01

    Full Text Available O presente artigo tem como base a interdisciplinaridade e apresenta relações pertinentes entre design, moda e arquitetura, focando nas similaridades durante o processo projetual e de criação de produtos dentro dessas áreas. O objetivo é expor as proximidades e interferências que essas disciplinas possuem em seus processos de criação e como os profissionais atuam em cada segmento e como buscam elementos nas outras disciplinas para criar seus produtos. Também é relevante o esclarecimento de que os profissionais podem e devem buscar referências em outras áreas distintas, pois muitas vezes, elas são mais acessíveis e parecidas do que se imagina. Para o desenvolvimento deste estudo foram investigados e analisados os processos de criação de duas profissionais da área de projeto, a arquiteta italiana, naturalizada brasileira, Lina Bo Bardi e a estilista brasileira Glória Coelho. O intuito deste trabalho é empregar o conhecimento obtido durante os cursos de arquitetura e urbanismo e de design de moda para investigar os processos de criação nos dois campos e fazer uma relação entre os dois mostrando suas semelhanças e contribuições entre si.

  8. Ultrastructural analysis of glands located in the wall of the congenital fistulae of the lower lip of patients with Van der Woude syndrome Análise ultra-estrutural das glândulas localizadas na parede da fístula congênita de lábio inferior de pacientes com a síndrome de Van der Woude

    Directory of Open Access Journals (Sweden)

    Mirian Aparecida Onofre

    2003-09-01

    Full Text Available The objective of the present study was to evaluate the glands of wall of congenital fistulae of the lower lip with the transmission electron microscope in order to characterize their microstructural pattern. Thin section of Araldite resin embedded congenital fistulae of the lower lip of four patients with Van der Woude syndrome from the Hospital de Reabilitação de Anomalias Craniofaciais da Universidade de São Paulo, Bauru, SP, were analyzed with a transmission electron microscope. The results showed that the glands were mostly made by typical mucous acini exhibiting, with certain frequency, myoepithelial cells surrounding them. In some of lobules, a few acini smaller than the typical mucous, showed granules of moderate electron density or containing a dense core or exhibiting small dense spherule and predominance granular material. These granules resemble to described recently by others in various human minor salivary glands. We concluded that glands associated with congenital fistula of lower lip of patients with Van der Woude syndrome, in spite of being located in vermilion border of the lip, showed at the transmission electron microscope characteristics of labial minor salivary gland, i.e, are mostly mucous with a few seromucous units, while typical seromucous demilunes are not present.O objetivo do presente estudo foi avaliar a ultraestrutura de glândulas da parede de fístula congênita de lábio inferior ao microscópio eletrônico de transmissão para caracterizar seu padrão microestrutural. Deste modo, as fístulas congênitas de 4 pacientes com a síndrome de Van der Woude do Hospital de Reabilitação de Anomalias Craniofaciais da Universidade de Sâo Paulo, Bauru, SP, foram processadas para inclusão em resina Araldite e os cortes finos foram analisados no microscópio eletrônico de transmissão. Os resultados mostraram que as glândulas estavam constituídas por ácinos mucosos típicos exibindo com certa freqüência c

  9. Immunization with a Double-Mutant (R192G/L211A) of the Heat-Labile Enterotoxin of Escherichia coli Offers Partial Protection against Campylobacter jejuni in an Adult Mouse Intestinal Colonization Model.

    Science.gov (United States)

    Albert, M John; Haridas, Shilpa; Ebenezer, Mathew; Raghupathy, Raj; Khan, Islam

    2015-01-01

    We have previously shown that antibodies to cholera toxin (CT) reacted with the major outer membrane proteins (MOMPs) from Campylobacter jejuni strains on Western blot. Further, oral immunization with CT significantly protected against challenge with C. jejuni in an adult mouse colonization model of infection. CT and the heat-labile enterotoxin (LT) of enterotoxigenic Escherichia coli are structurally and functionally related. LT and its mutants including the double-mutant LT (R192G/L211A) (dmLT), are powerful mucosal adjuvants. Unlike LT which is reactogenic, dmLT has been shown to be safe for human use. In the current study, we determined whether rabbit anti-dmLT antibodies reacted with MOMPs from C. jejuni strains and whether immunization with dmLT would afford protection against C. jejuni. On Western blot, the MOMPs from C. jejuni 48 (Penner serotype O:19), C. jejuni 75 (O:3) and C. jejuni 111 (O:1,44) were probed with rabbit antibodies to dmLT or LT-E112K (a non-toxic LT mutant), which showed a lack of reaction. Adult BALB/c mice were orally immunized with dmLT and orally challenged with C. jejuni 48 or 111. Protection from colonization with the challenge bacteria was studied by enumerating Campylobacter colonies in feces daily for 9 days. Vaccination produced robust serum and stool antibody responses to dmLT and no antibody responses to C. jejuni MOMP. Vaccinated mice showed reduced colonization and excretion of both challenge strains compared to control mice. However, the differences were not statistically significant. The protective efficacy of the dmLT vaccine varied from 9.1% to 54.5%. The lack of cross-reaction between the MOMP and dmLT suggests that protection is not mediated by cross-reacting antibodies, but may be due to activation of innate immunity. As dmLT is safe for humans, it could be incorporated into a C. jejuni vaccine to enhance its efficacy.

  10. Immunization with a Double-Mutant (R192G/L211A of the Heat-Labile Enterotoxin of Escherichia coli Offers Partial Protection against Campylobacter jejuni in an Adult Mouse Intestinal Colonization Model.

    Directory of Open Access Journals (Sweden)

    M John Albert

    Full Text Available We have previously shown that antibodies to cholera toxin (CT reacted with the major outer membrane proteins (MOMPs from Campylobacter jejuni strains on Western blot. Further, oral immunization with CT significantly protected against challenge with C. jejuni in an adult mouse colonization model of infection. CT and the heat-labile enterotoxin (LT of enterotoxigenic Escherichia coli are structurally and functionally related. LT and its mutants including the double-mutant LT (R192G/L211A (dmLT, are powerful mucosal adjuvants. Unlike LT which is reactogenic, dmLT has been shown to be safe for human use. In the current study, we determined whether rabbit anti-dmLT antibodies reacted with MOMPs from C. jejuni strains and whether immunization with dmLT would afford protection against C. jejuni. On Western blot, the MOMPs from C. jejuni 48 (Penner serotype O:19, C. jejuni 75 (O:3 and C. jejuni 111 (O:1,44 were probed with rabbit antibodies to dmLT or LT-E112K (a non-toxic LT mutant, which showed a lack of reaction. Adult BALB/c mice were orally immunized with dmLT and orally challenged with C. jejuni 48 or 111. Protection from colonization with the challenge bacteria was studied by enumerating Campylobacter colonies in feces daily for 9 days. Vaccination produced robust serum and stool antibody responses to dmLT and no antibody responses to C. jejuni MOMP. Vaccinated mice showed reduced colonization and excretion of both challenge strains compared to control mice. However, the differences were not statistically significant. The protective efficacy of the dmLT vaccine varied from 9.1% to 54.5%. The lack of cross-reaction between the MOMP and dmLT suggests that protection is not mediated by cross-reacting antibodies, but may be due to activation of innate immunity. As dmLT is safe for humans, it could be incorporated into a C. jejuni vaccine to enhance its efficacy.

  11. Frecuencia de las mutaciones en los genes BRCA en mujeres con agregación familiar de cáncer de glándula mamaria/ovario

    National Research Council Canada - National Science Library

    Jennyfer Dahianna Benavides-Cerquera; Mabel Elena Bohórquez-Lozano; Carlos Fernando Prada-Quiroga; Luis Carvajal-Carmona; María Magdalena Echeverry de Polanco

    2016-01-01

    Objetivo: Reunir evidencias de la variación en la frecuencia de las mutaciones de BRCA1 y BRCA2 y la historia familiar en pacientes con cáncer de glándula mamaria (CGM) y cáncer de ovario (CO) de diferentes orígenes geográficos. Método...

  12. Adenoma pleomorfo gigante de glándula salivar menor: Extirpación a través de un abordaje transoral Giant pleomorphic adenoma of minor salivary gland: Transoral resection

    Directory of Open Access Journals (Sweden)

    C. de Paula Vernetta

    2008-06-01

    Full Text Available El adenoma pleomorfo es un tumor benigno de las glándulas salivares formado por la combinación de elementos epiteliales y mesenquimales. Generalmente constituyen el 60-70% de los tumores de la glándula parótida y el 40-60% de los de glándula submaxilar. Menos frecuentemente es su desarrollo a partir de una glándula salivar menor, presentándose como una masa intraoral dependiente de paladar o labio. Se expone el caso de un adenoma pleomorfo gigante de paladar duro y su exéresis por la vía transoral.Pleomorphic adenoma is a benign tumor of the salivary glands that consists of a combination of epithelial and mesenchymal elements. The tumor most commonly arises from the parotid (60- 70% or submandibular glands. It develops less frequently on a minor salivary gland, presenting as an intraoral mass dependent on the palate or lip. A case is reported of giant pleomorphic adenoma resected using a transoral approach.

  13. Técnica para extraer cantidad apreciable de hemolinfa y sacar fácilmente glándulas salivales en ninfas de quinto estadio y adultos de Rhodnius Prolixus

    Directory of Open Access Journals (Sweden)

    Ernesto Osorno Mesa

    1965-07-01

    Full Text Available Se describen minuciosamente los diferentes pasos para extracción de hemolinfa con micropipetas, en ninfas de 5º estadio y adultos de Rhodnius prolixus, lo mismo que para el aislamiento de las glándulas salivales. Se anotan las ventajas y aplicaciones de estas técnicas, en investigaciones parasitológicas, especialmente sobre Trypanosomiasis.

  14. Variation in macronutrients in human bank milk: influencing factors and implications for human milk banking

    DEFF Research Database (Denmark)

    Michaelsen, K F; Skafte, L; Badsberg, J H

    1990-01-01

    with a high P content, we have developed a "high-protein" milk with a P content of about 12 g/L (true protein) and an E content of about 725 kcal/L. Thus, by continuous monitoring of macronutrient content in human bank milk it is possible to develop a "high-protein" milk with sufficient P and E content......Protein (P), fat (F), and carbohydrate (C) concentration in expressed human bank milk was determined by infrared analysis of 2,554 samples from 224 mothers. The mean contents of P, F, C, and energy (E, calculated from P, F, and C) were 9.0 g/L, 39.0 g/L, 71.9 g/L, and 696 kcal/L, respectively....... The main results were as follows: the P and F contents increased slightly with increasing body mass index of the mother, the P content decreased with increasing amounts of milk delivered to the milk bank, and the F content was higher in mothers delivering large amounts of milk. By selecting incoming milk...

  15. Configuração glótica em tocadores de instrumento de sopro Glottic configuration in wind instrument players

    Directory of Open Access Journals (Sweden)

    Claudia Alessandra Eckley

    2006-02-01

    Full Text Available O conhecimento dos problemas de saúde relacionados à voz ocupacional torna-se cada vez mais importante à medida que mais indivíduos usam a voz como instrumento de trabalho. Os músicos tocadores de instrumento de sopro são um grupo bastante específico de indivíduos que usa o trato vocal intensamente no exercício de suas atividades profissionais. Curiosamente, pouco ou nada temos relatado sobre a atuação direta da laringe nesta modalidade profissional. OBJETIVO: O objetivo do atual estudo foi avaliar o comportamento da laringe e do trato vocal de músicos tocadores de instrumento de sopro. MATERIAL E MÉTODO: Foram estudados 10 indivíduos tocadores profissionais de instrumento de sopro através de videonasofibrolaringoscopia, sendo observados o comportamento da laringe, faringe e língua durante o tocar do instrumento. RESULTADOS: Em todos os participantes deste estudo observamos que os tons musicais foram produzidos durante a adução das pregas vocais. O relato de maior dificuldade técnica de tocar determinada peça musical estava relacionado a uma maior tensão glótica (constricção látero-lateral e supraglótica. CONCLUSÕES: A glote participa ativamente da produção sonora do instrumento de sopro e que alterações na configuração glótica podem interferir na produção sonora musical final. Estes conhecimentos sugerem a necessidade de incluir os músicos tocadores de instrumento de sopro no grupo dos chamados profissionais da voz.INTRODUCTION: Knowledge of occupational voice disorders has gained increased importance as more individuals rely on their voices for their work. Wind instrument players are a specific group of individuals that present intensive use of the vocal tract associated with blowing their instrument. Interestingly, only a small number of reports focus on the laryngeal function of such professionals. AIM: The current study evaluated the laryngeal and vocal tract movement of wind instrument players. MATERIAL

  16. Farelo de glúten de milho para vacas leiteiras em pastos de azevém anual Corn gluten meal to dairy cows grazing italian ryegrass

    Directory of Open Access Journals (Sweden)

    Henrique Mendonça Nunes Ribeiro Filho

    2009-07-01

    Full Text Available Neste trabalho, foi avaliado o efeito da suplementação com proteína de baixa degradabilidade ruminal (PNDR para vacas leiteiras em pastos de azevém anual (Lolium multiflorum Lam.. Foram utilizadas 12 vacas da raça Holandesa no terço médio de lactação. Os tratamentos avaliados foram zero e 4,0kg vaca-1 dia-1 de uma mistura 60:40 de grão de milho + farelo de glúten de milho, em três períodos de avaliação de 15 dias. Os animais foram distribuídos em quatro lotes uniformes (dois por tratamento e alocados em piquetes de azevém manejado no método rotativo, com uma oferta de 24kg de MS vaca-1 dia-1. A massa de forragem (2.325kg MS ha-1 e a altura (15,8cm antes da entrada dos animais foram semelhantes nos piquetes dos animais suplementados e não suplementados. Da mesma forma, os teores médios de PB e FDN da forragem ingerida foram semelhantes entre tratamentos (18,9% e 53,0%, respectivamente. O consumo de MS da forragem não variou com o uso da suplementação (média=8,5kg vaca-1 dia-1. O consumo de MS total aumentou 3,0kg vaca-1 dia-1 e o de energia metabolizável aumentou 42MJ vaca-1 dia-1. A produção de leite aumentou 4,0kg vaca-1 dia-1 com o uso da suplementação, mas os teores de gordura e de proteína não variaram entre tratamentos, sendo observados valores médios de 32,8g kg-1 e 29,2g kg-1, respectivamente. Em baixa oferta de forragem, a suplementação com alimento concentrado (22% PB contendo farelo de glúten de milho eleva a produção de leite na ordem de 1kg por kg de concentrado. A resposta a esse tipo de suplementação em condição de oferta de forragem não-limitante deve ser estudada.The aim of this research was to evaluate the effect of undegradable protein supplementation to dairy cows grazing Italian ryegrass. Twelve Holstein cows in the mid lactation were used and treatments were 0 (zero and a mixture (60:40 of ground corn + corn gluten meal (4.0kg cow day-1 evaluated along three periods of fifteen days

  17. Improved fatigue behavior of low-carbon steel 20GL by applying ultrasonic impact treatment combined with the electric discharge surface alloying

    Energy Technology Data Exchange (ETDEWEB)

    Mordyuk, B.N., E-mail: mordyuk@imp.kiev.ua [Kurdyumov Institute for Metal Physics, NAS of Ukraine, 36 Academician Vernadsky blvd., UA-03142, Kyiv (Ukraine); Prokopenko, G.I.; Volosevich, P.Yu. [Kurdyumov Institute for Metal Physics, NAS of Ukraine, 36 Academician Vernadsky blvd., UA-03142, Kyiv (Ukraine); Matokhnyuk, L.E.; Byalonovich, A.V. [Pisarenko Institute for Strength of Materials, NAS of Ukraine, 2 Timiryazevs’ka str., UA-01014, Kyiv (Ukraine); Popova, T.V. [Ukrainian Research Institute Rail carriage building, 33 Prikhodko str., UA-39621, Kremenchuk (Ukraine)

    2016-04-06

    The effects of severe plastic deformation induced by ultrasonic impact treatment (UIT) and the electric discharge surface alloying (EDSA) with chromium on the stress-controlled fatigue response of low-carbon steel 20GL are studied. The surface microrelief and integrity were analyzed using light microscopy and scanning electron microscopy (SEM). The structural formations in the sub-surface layers were characterized by means of X-ray diffraction analysis and transmission electron microscopy (TEM). The steel specimens underwent UIT, and complex UIT+EDSA and UIT+EDSA+UIT processes demonstrate the fatigue strength magnitudes increased respectively by ~15, ~5 and ~30% on the base of 10{sup 7} cycles in comparison with that for the pristine specimen. SEM analysis of fracture surfaces reveals the subsurface crack nucleation in the UIT-processed specimens instead of superficial crack initiation observed in the pristine and EDSA-processed ones. TEM studies demonstrate that a dislocation-cell structure forms in ferrite grains and partial dissolution of cementite occurs in pearlite grains both at the surface after UIT and in the layer at a depth of 15–25 µm after the UIT+EDSA+UIT process. The enhanced fatigue strength and prolonged lifetime of the low-carbon steel specimens after UIT and UIT+EDSA+UIT processes are concluded to be associated with the subsurface crack nucleation achieved by the following factors: (i) minimized surface roughness and improved integrity of the modified layer; (ii) compressive residual stresses; and (iii) surface hardening coupled with the alloying by chromium and with the formation of the dislocation-cell structure containing the cell walls impenetrable to moving dislocations at cyclic loading.

  18. Estudio del efecto de los esteroles aislados de la planta solanum nudum en la membrana del glóbulo rojo humano

    Directory of Open Access Journals (Sweden)

    Silvia Blair Trujillo

    2004-02-01

    Full Text Available

    En el mundo existen pocas alternativas terapéuticas para malaria. El Grupo Malaria ha aislado esteroides de la planta Solanum nudum, usada para el tratamiento de fiebres por la edicina tradicional colombiana, y ha encontrado que tienen ctividad antimalárica in vitro e in vivo contra P. falciparum, P. vivax y P. berghei. Los compuestos aislados de S. nudum son de tipo esteroidal y se conoce que los esteroles pueden afectar la elación colesterol/fosfolípidos en la membrana del eritrocito 1, igualmente, pueden cambiar su permeabilidad para Na+ y + (2. En los eritrocitos parasitados con Plasmodium la edistribución del colesterol en la membrana y la presencia de roteínas del parásito aumentan la permeabilidad. En la membrana el eritrocito se presentan microdominios: arreglos de ípidos y colesterol a los cuales se anclan las proteínas a través el glicosilfosfatidilinositol. Se ha observado como la extracción el colesterol de los microdominios del eritrocito interfiere en la invasión del glóbulo rojo por Plasmodium (3, sugiriendo que pueden ser aprovechados funcionalmente como blanco erapéutico. El objetivo de este trabajo es determinar el efecto de los esteroles de la planta Solanum nudum en la membrana el eritrocito humano normal e infectado con P. falciparum, mediante el análisis de permeabilidad y de microdominios.

     

     

  19. Alterações histopatológicas da glândula mamária e qualidade do leite de cabras naturalmente infectadas com o CAEV

    Directory of Open Access Journals (Sweden)

    R.Q. Bezerra Júnior

    2012-12-01

    Full Text Available Avaliou-se a influência do vírus da CAE nas características físico-químicas de amostras de leite de 54 cabras, sem predileção racial, distribuindo-as em dois grupos: cabras positivas e negativas para o teste de imunodifusão em gel de agarose. As amostras de leite foram submetidas à análise ultrassônica para obtenção de parâmetros físico-químicos - gordura, extrato seco, proteínas, lactose e densidade; realização de microbiologia - bactérias mesófilas (UCF/mL. Foram coletadas amostras de tecido mamário para exame histopatológico e imunohistoquímica. Não houve diferença significativa das características avaliadas entre os dois grupos; no microbiológico, não houve relação direta da presença de mesófilas associada à infecção pelo CAEV. Na histopatologia, observaram-se áreas com infiltração celular de monócitos, polimorfonucleares, plasmócitos, fibrose, ausência de morfologia normal do parênquima mamário, denotando processo inflamatório crônico; e foi confirmada a presença do vírus na glândula pela imunohistoquímica. Os resultados não mostraram relação direta da incidência da CAE como fator negativo no desenvolvimento do rebanho.

  20. Primary plasmacytoma of the thyroid gland: a case report of a rare neoplasia Plasmocitoma da glândula tireoide: relato de caso de uma rara neoplasia

    Directory of Open Access Journals (Sweden)

    Eduardo Cambruzzi

    2012-08-01

    Full Text Available Extramedullary plasmacytoma comprises 3%-5% of all plasma cell neoplasms, and approximately 80% of the cases occur in the upper respiratory tract. Primary thyroid plasmacytomas (PTP are rare tumors. The authors report a case of PTP in a male patient with dyspnea and dysphagia. Physical examination and computerized tomography (CT scan revealed a solid tumor affecting the thyroid gland, measuring 12 cm in its greatest dimension. Surgical biopsy was performed. Microscopy revealed a hypercellular malignant neoplasm composed of round plasmacytoid cells arranged in solid nests, which showed a positive immunoexpression for CD138, epithelial membrane antigen (AME, kappa light chains and multiple myeloma oncogene 1 (MUM1. The diagnosis of PTP was accordingly established.O plasmocitoma extramedular constitui 3%-5% de todas as neoplasias de plasmócitos e aproximadamente 80% dos casos ocorrem no trato respiratório superior. Plasmocitomas primários da tireoide (TPP são tumores raros. Os autores relatam um caso de TPP em um paciente masculino referindo dispneia e disfagia. O exame físico e a tomografia computadorizada revelaram tumor sólido comprometendo a glândula tireoide, medindo 12 cm na maior dimensão, o qual foi submetido à biópsia cirúrgica. À microscopia, foi identificada neoplasia maligna hipercelular constituída por células redondas/plasmocitoides dispostas em ninhos sólidos, as quais exibiam imunoexpressão positiva para CD138, antígeno da membrana epitelial (AME, cadeias leves kappa e oncogene mieloma múltiplo 1 (MUM1. O diagnóstico de TPP foi, então, estabelecido.

  1. "Hard-earned wisdom: Exploratory processing of difficult life experience is positively associated with wisdom": Correction to Weststrate and Glück (2017).

    Science.gov (United States)

    2017-06-01

    Reports an error in "Hard-earned wisdom: Exploratory processing of difficult life experience is positively associated with wisdom" by Nic M. Weststrate and Judith Glück (Developmental Psychology, 2017[Apr], Vol 53[4], 800-814). The affiliation of Nic M. Weststrate was inadvertently set as "Relationships and Life Experiences" in the article byline. It should have been "University of Toronto". The online version of this article has been corrected. (The following abstract of the original article appeared in record 2017-12497-007.) Laypersons and experts believe that wisdom is cultivated through a diverse range of positive and negative life experiences. Yet, not all individuals with life experience are wise. We propose that one possible determinant of growth in wisdom from life experience is self-reflection. In a life span sample of adults (N = 94) ranging from 26 to 92 years of age, we examined wisdom's relationship to self-reflection by investigating "why" people report reflecting on the past (i.e., reminiscence functions) and "how" they reflect within autobiographical memories of difficult life events (i.e., autobiographical reasoning). We assessed wisdom using self-report, performance, and nomination approaches. Results indicated that wisdom was unrelated to the frequency of self-reflection; however, wiser people differed from others in their (a) reasons for reminiscence and (b) mode of autobiographical reasoning. Across 3 methods for assessing wisdom, wisdom was positively associated with exploratory processing of difficult life experience (meaning-making, personal growth), whereas redemptive processing (positive emotional reframing, event resolution) was positively associated with adjustment. This study suggests that developmental pathways in the wake of adversity may be partially determined by how individuals self-reflectively process significant life experiences. (PsycINFO Database Record (c) 2017 APA, all rights reserved).

  2. Targeted mass spectrometry analysis of the proteins IGF1, IGF2, IBP2, IBP3 and A2GL by blood protein precipitation.

    Science.gov (United States)

    Such-Sanmartín, Gerard; Bache, Nicolai; Callesen, Anne K; Rogowska-Wrzesinska, Adelina; Jensen, Ole N

    2015-01-15

    Biomarker analysis of blood samples by liquid chromatography (LC) mass spectrometry (MS) is extremely challenging due to the high protein concentration range, characterised by abundant proteins that suppress and mask other proteins of lower abundance. This situation is further aggravated when using fast high-throughput methods, which are necessary for analysis of hundreds and thousands of samples in clinical laboratories. The blood proteins IGF1, IGF2, IBP2, IBP3 and A2GL have been proposed as indirect biomarkers for detection of GH administration and as putative biomarkers for breast cancer diagnosis. We describe a sensitive and scalable method to quantify these 5 proteins of medium and low abundance by selected reaction monitoring (SRM) LC-MS/MS analysis in blood samples. Our method requires 7μL of plasma and reaches a throughput of up to ca. 80 analyses per day. It includes an initial protein precipitation protocol optimised for extraction of low mass proteins from blood samples for reduced signal suppression and increased sensitivity in LC-MS/MS. We benchmarked this method for the analysis of 40 individual blood samples including 20 patients diagnosed with breast cancer. The interest for MS-based biomarker analysis in body fluids is steadily increasing as proteomics methodology translates into clinical laboratories. We describe a method for detection of 5 distinct proteins of low mass and medium to low abundance, which are of interest in anti-doping and clinical analysis. The analytical setup is simple and robust and is suitable for high-throughput instrument configurations. Copyright © 2014 Elsevier B.V. All rights reserved.

  3. Elaboração e avaliação da qualidade de macarrão isento de glúten

    Directory of Open Access Journals (Sweden)

    Letícia Tomicki

    2015-07-01

    Full Text Available Com a intenção de minimizar a carência de produtos isentos de glúten e tendo em vista o crescente aumento de celíacos, a elaboração de produtos diferenciais a base de farinha de arroz e milho torna-se uma alternativa de consumo e comercialização. Assim, este trabalho teve como objetivo avaliar os efeitos da adição de farinha de arroz e milho, e emulsificante nas características de qualidade (teste de cozimento, textura instrumental - firmeza e aceitabilidade e físico-químicas (proteínas, lipídios, carboidratos, minerais e fibra alimentar de formulações de macarrão, mediante o emprego de metodologia de planejamento de experimentos, fixando a concentração de ovos in natura (12%, m/m e água (20%, v/m. A formulação com 40g de farinha de milho, 60g de farinha de arroz e 0,4g de SSL por 100g de farinha mista foi a que apresentou melhor resistência ao cozimento, menores perdas de sólidos na água de cocção (2,12% m/m, textura (30,40g seg-1 e aceitabilidade superior a 85 %. Além disso, o uso de farinha de arroz, associado à farinha de milho, proporciona fonte de minerais (152mg de K, 1,55mg de Fe, 46mg de Mg, 20mg de Ca por 100g, proteínas (~7%, carboidratos (52% e fibras (~1%, importante suprimento nutricional para celíacos, por estes apresentarem perdas significativas destes nutrientes, devido à diarreia e à absorção restrita, característica da doença.

  4. In vivo visualization of GL261-luc2 mouse glioma cells by use of Alexa Fluor-labeled TRP-2 antibodies.

    Science.gov (United States)

    Fenton, Kathryn E; Martirosyan, Nikolay L; Abdelwahab, Mohammed G; Coons, Stephen W; Preul, Mark C; Scheck, Adrienne C

    2014-02-01

    For patients with glioblastoma multiforme, median survival time is approximately 14 months. Longer progression-free and overall survival times correlate with gross-total resection of tumor. The ability to identify tumor cells intraoperatively could result in an increased percentage of tumor resected and thus increased patient survival times. Available labeling methods rely on metabolic activity of tumor cells; thus, they are more robust in high-grade tumors, and their utility in low-grade tumors and metastatic tumors is not clear. The authors demonstrate intraoperative identification of tumor cells by using labeled tumor-specific antibodies. GL261 mouse glioma cells exhibit high expression of a membrane-bound protein called second tyrosinase-related protein (TRP-2). The authors used these cells to establish an intracranial, immunocompetent model of malignant glioma. Antibodies to TRP-2 were labeled by using Alexa Fluor 488 fluorescent dye and injected into the tail vein of albino C57BL/6 mice. After 24 hours, a craniotomy was performed and the tissue was examined in vivo by using an Optiscan 5.1 handheld portable confocal fiber-optic microscope. Tissue was examined ex vivo by using a Pascal 5 scanning confocal microscope. Labeled tumor cells were visible in vivo and ex vivo under the respective microscopes. Fluorescently labeled tumor-specific antibodies are capable of binding and identifying tumor cells in vivo, accurately and specifically. The development of labeled markers for the identification of brain tumors will facilitate the use of intraoperative fluorescence microscopy as a tool for increasing the extent of resection of a broad variety of intracranial tumors.

  5. Considerações gerais e específicas sobre as glândulas cutâneas sexuais dos Lepidopteras

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    Rudolf Barth

    1980-12-01

    Full Text Available No presente estudo podemos ressaltar alguns dados e observações mostrando que estes órgãos glandulares e suas secreções atraentes e excitantes têm um papel biológico essencial, sendo sua função regular e segura de importância decisiva para a sobrevivência de muitas espécies de Lepidoptera. São ressaltados os seguintes dados: a a variabilidade de formação dos órgãos e seus componentes; b a localização no corpo para obter o máximo de proteção e efeito; c a alta sensibilidade do sistema sensorial do sexo oposto; d a distribuição das sensilas sobre as antenas como expressão da função do olfato e da importância biológica da secreção. Além disso, mostramos os resultados que o senso olfatório dos Lepidoptera - e isto pode-se estender a respeito do plano funcional geral de todos os Insecta e Arthropoda - é inestimavelmente mais sensível do que aquele dos órgãos onde a molécula tem que se difundir via um líquido intermediário, de via direta e seca nos Insecta. A continuação desta interpretação está fora do nosso alcance como histólogos, sendo reservada para a parte filosófica da ciência. hipoderme e suas glândulas em geral (dos insecta.

  6. Tumor trabecular hialinizante de glándula tiroides: un reto diagnóstico, a propósito de un caso

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    Julio Cesar Díaz Acosta

    2014-08-01

    Full Text Available Introducción: El tumor trabecular hialinizante de tiroides es una condición clínica incidental, infrecuente, de comportamiento incierto, representa un reto diagnóstico y terapéutico debido a que es difícil diferenciarlo de otros tumores tiroideos como el carcinoma papilar y medular por sus similitudes histológicas. Reporte de caso: Paciente de 53 años quien acudió a consulta por crecimiento de la glándula tiroidea y disfagia. Se realizaron estudios diagnósticos previos los cuales orientaron hacia una condición benigna sin embargo por progresión de síntomas se decidió realizar tiroidectomía total. La histopatología de la masa reportó carcinoma medular y en la inmunohistoquímica como hallazgo incidental se realiza diagnóstico final de adenoma trabecular hialinizante de tiroides. Discusión: El tumor trabecular hialinizante de tiroides presenta un desafío continuo, por lo cual es necesario realizar pruebas inmunohistoquímicas que permitan realizar la diferenciación. El abordaje quirúrgico no es claro debido a su comportamiento incierto y a que las publicaciones que existen no son claras acerca del pronóstico y manejo. Conclusiones: El tumor trabecular hialinizante de tiroides es una enfermedad con bajas tasas de incidencia, frecuentemente mal diagnosticado y sobre el cual no existe información clara acerca de su manejo. El seguimiento clínico de estos pacientes y futuros estudios observacionales nos permitirá obtener conclusiones a futuro sobre su comportamiento y pronóstico.

  7. Rabdomiólise por síndrome compartimental glútea após cirurgia bariátrica: relato de caso Rabdomiolisis por síndrome compartimental glúteo después de cirugía bariátrica: relato de caso Rhabdomyolysis secondary to gluteal compartment syndrome after bariatric surgery: case report

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    Márcio Luiz Benevides

    2006-08-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: A cirurgia bariátrica tornou-se rotineira e muitas complicações têm sido relatadas. O objetivo deste relato foi apresentar um caso de síndrome compartimental glútea que evoluiu para insuficiência renal aguda após cirurgia bariátrica e discutir aspectos do diagnóstico e condutas profilática e terapêutica. RELATO DO CASO: Paciente do sexo masculino, 42 anos, branco, índice de massa corporal (IMC 43, estado físico ASA II, submetido à cirurgia bariátrica tipo duodenal switch, sob anestesia geral associada à anestesia peridural. O procedimento transcorreu sem intercorrências. O tempo anestésico-cirúrgico foi de 3 horas e 30 minutos. No primeiro dia do pós-operatório o paciente apresentou dor na região lombossacral e nas nádegas, além de parestesia nos membros inferiores na distribuição do nervo isquiático. Durante o exame, as nádegas apresentavam discreta palidez, tensas, edemaciadas, dolorosas à palpação e à movimentação. Foi diagnosticada síndrome compartimental glútea que evoluiu com rabdomiólise e insuficiência renal aguda. Houve recuperação da função renal e nenhuma seqüela motora ou sensitiva foi detectada. CONCLUSÕES: Os pacientes obesos mórbidos submetidos à cirurgia bariátrica podem apresentar síndrome compartimental glútea. Quando não diagnosticada e tratada precocemente, podem evoluir com rabdomiólise e insuficiência renal aguda que representam grave ameaça à vida.JUSTIFICATIVA Y OBJETIVOS: La cirugía bariátrica se convirtió en rutina y en algo muy complicado para ser relatada. El objetivo de este relato fue el de presentar un caso de síndrome compartimental glútea que evolucionó para la insuficiencia renal aguda después de la cirugía bariátrica y discutir aspectos del diagnóstico y conductas profiláctica y terapéutica. RELATO DEL CASO: Paciente del sexo masculino, 42 años, blanco, índice de masa corporal (IMC 43, estado físico ASA II, sometido

  8. Factores de riesgo cardiovasculares y su relación con las alteraciones del metabolismo de los glúcidos y lípidos

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    Lilian Leguen Gulgar

    2014-12-01

    Full Text Available Fundamento: las enfermedades cardiovasculares han constituido la primera causa de muerte en Cuba, donde son escasos los estudios realizados sobre los factores de riesgo cardiovasculares emergentes como predictores del riesgo cardiovascular. Objetivo: determinar la asociación entre los factores de riesgo cardiovasculares con las alteraciones en el metabolismo de los glúcidos y los lípidos Métodos: se realizó un estudio descriptivo y correlacional a partir de una muestra de 105 personas de ambos sexos del universo de 346 trabajadores de la Universidad de Ciencias Médicas de Cienfuegos. El estudio se realizó entre los meses de junio 2011 a julio de 2012. Las variables analizadas fueron: edad, sexo, presión arterial, cintura abdominal, hábito de fumar, glucemia en ayunas, triglicéridos, colesterol, HDL colesterol, apolipoproteínas A y B, cocientes CT/cHDL y cociente Apo B/Apo A-I. Resultados: los mayores de 45 años del sexo femenino presentaron mayor prevalencia de la circunferencia abdominal alterada (60,0 %, hipertensión arterial (46,7 % y diabetes mellitus tipo 2 (54,3 % con hipertrigliceridemia (43,3 %, valores bajos de HDLc (36,7 % y 2,8 mayor probabilidad de presentar circunferencia abdominal alterada; el 66,7 % de los diabéticos presentaron bajos valores de HDLc, el 33,3 % hipertrigliceridemia y el 66,7 % cociente colesterol total/HDLc elevado. Conclusiones: se observó asociación entre la edad mayor de 45 años, el sexo femenino, la obesidad, hipertensión arterial y diabetes mellitus tipo 2, mayor prevalencia de hipertrigliceridemia y bajos valores de HDLc en los obesos y en los diabéticos además de observarse mayor riesgo de bajos valores de HDLc y elevado cociente de colesterol total/HDL colesterol.

  9. Obtention and characterization of gluten-free baked products Obtenção e caraterização de produtos panificados livres de glúten

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    Cristiane Schamne

    2010-09-01

    Full Text Available This work had as objective the development of gluten-free breads and muffins using rice flour and maize and cassava starches. From seven samples resulting from a Simplex-Centroid design, the sensory and instrumental analyses of specific volume, elasticity, and firmness were performed. For the sensory analysis, the optimized formulation contained 50% of rice flour and 50% of cassava starch, and for the instrumental evaluation, the optimal simultaneous point for the three conducted analyses were 20% of rice flour, 30% of cassava starch, and 50% of maize starch. A comparative analysis of specific volume, elasticity, firmness, and triangular test was performed with pre-baked, baked, and frozen bread. Physicochemical, nutritional, and microbiological analyses were performed for both bread and muffin according to the Brazilian legislation.Este trabalho teve como objetivo o desenvolvimento de pães e bolos tipo muffin livres de glúten, usando farinha de arroz e amidos de milho e de mandioca. A partir de sete amostras resultantes de um delineamento Simplex-Centroide, foram realizadas análises sensoriais e instrumentais de volume específico, elasticidade e firmeza. Para a análise sensorial, a formulação ótima de mistura encontrada foi 50% de creme de arroz e 50% de amido de mandioca e, para a análise instrumental, o ponto ótimo simultâneo das três análises realizadas foi 20% de creme de arroz, 30% de amido de mandioca e 50% de amido de milho. Análises comparativas foram feitas para volume específico, elasticidade e firmeza, e teste triangular entre o pão assado e o pão pré-assado e congelado. Para ambos os produtos, foram realizadas análises físico-químicas e nutricionais, as quais estão de acordo com a legislação vigente.

  10. Gluten free cakes made with broken rice and cassava peelBolos sem glúten a base de arroz quebrado e casca de mandioca

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    Thaísa Anders Carvalho Souza

    2013-05-01

    poderia ser aproveitada melhor, em função do grande montante produzido e de seu valor nutricional. O objetivo deste trabalho foi verificar a viabilidade da aplicação de casca de mandioca desidratada na formulação de misturas de bolos sem glúten em substituição a farinha de arroz. Os bolos foram formulados com diferentes níveis de substituição da farinha de arroz (FA pela farinha da casca de mandioca (FCM (0%, 25%, 50%, 75% e 100%, com base em volume. Para obtenção da farinha, a casca de mandioca foi desidratada e moída. As amostras de farinhas e bolos foram submetidas a análises físicas, de composição centesimal, e microbiológicas (Bacilus cereus, coliformes totais e a 45 °C, Salmonella sp, bolores e leveduras e clostrídios sulfitos redutores e aceitação sensorial. Os bolos diferiram (p?0,05 no teor de umidade (29,6 a 41,8 g.100g-1, que foi maior com o aumento o aumento da proporção da FCM. A mesma tendência foi observada no coeficiente de índice de rendimento (0,72 a 0,84, e nos teores de cinzas (3,1 a 4,8 g.100g-1, lipídeos (8,6-16,7 g.100g-1 e fibra alimentar total (4,1 a 19,3 g.100g-1 e insolúvel (3,5 a 17,3 g.100g-1. Os bolos não apresentaram contaminação microbiológica. Em relação aos atributos sensoriais, os bolos tiveram a mesma aceitação (em torno de 6,8, e a maior intenção de compra (40% foi obtida pela amostra com 100% de substituição de FA por FCM. Os resultados permitem inferir que existe possibilidade da utilização da farinha de casca de mandioca como substituinte da farinha de arroz em formulações de bolos sem glúten em até 100% de substituição, uma vez que, as características nutricionais, microbiológicas e a aceitação sensorial foram satisfatórias.

  11. Tratamento radioterápico dos tumores glômicos do osso temporal Radiation therapy for glomus tumors of the temporal bone

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    Celso Dall'Igna

    2005-12-01

    Full Text Available O tratamento dos tumores glômicos tem sido motivo de controvérsia desde sua primeira publicação, podendo ser cirúrgico, radioterápico ou apenas expectante. OBJETIVO: O objetivo do estudo foi avaliar a efetividade e as complicações do tratamento radioterápico para esses tumores. FORMA DE ESTUDO: clínico com coorte transversal. MATERIAL E MÉTODO: Trata-se de uma coorte histórica de pacientes com tumor glômico jugular submetidos à radioterapia. Os critérios de controle da doença foram não haver progressão dos sintomas ou disfunção de nervos cranianos, sem aumento do tamanho da lesão ao exame físico ou controle radiológico. Avaliamos também a presença de seqüelas do tratamento. RESULTADOS: Foram incluídos 12 pacientes, sendo oito mulheres. O tempo de follow-up variou de 3 a 35 anos, com uma media de 11,6 anos. Os principais sintomas foram: hipoacusia, zumbido pulsátil e tontura ou vertigem. Os achados de exame físico mais freqüentes foram massa pulsátil retrotimpanica, paralisia facial e anacusia, sendo os tumores estadiados segundo a classificação proposta por Fisch. A radioterapia foi realizada com acelerador linear com doses variando de 4500-5500 Rads por 4-6 semanas. As seqüelas mais comuns foram a dermatite, estenose do conduto auditivo externo, anacusia e paralisia facial. DISCUSSÃO: Os sintomas e achados de exame físico e o método e dosagem da radioterapia não diferiram daqueles encontrados na literatura. Todos os pacientes tiveram melhora dos sintomas e apenas um não foi considerado como tendo controle da doença. As complicações do tratamento foram de pouca repercussão, com exceção da anacusia e da paralisia facial. CONCLUSÃO: A radioterapia é uma alternativa viável para o tratamento desses tumores pela boa resposta e baixo índice de complicações. Deve ser considerada especialmente em tumores mais avançados onde um procedimento cirúrgico pode trazer grande morbidade.The treatment of glomic

  12. Expression von Blutgruppenantigenen durch humane mesenchymale Stammzellen

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    Schüle, Michael Christian

    2013-01-01

    Die vorliegende experimentelle Arbeit befasst sich mit der Untersuchung der Expression von Blutgruppenantigenen durch humane mesenchymale Stammzellen (MSC) aus dem Knochenmark. Der Fokus lag hierbei auf den klinisch bedeutsamen Antigenen A, B, H, Rhesus-D, -C, -c, -E, -e, RhAG, K, k, Jka, Jkb und DARC. Die Expression wurde systematisch auf Genom-, Transkriptom- und Proteinebene evaluiert. Alle untersuchten MSC Populationen exprimierten FUT1, RHCE, KEL und Kidd (HUT11) mRNA. mRNA der AB0-Gl...

  13. Elastografia Acoustic Radiation Force Impulse (ARFI) e Doppler na avaliação das glândulas adrenais de cães hígidos

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    Fernandez, Stephanie [UNESP

    2016-01-01

    As glândulas adrenais exercem papel fundamental na manutenção da homeostasia tanto em humanos, como nos animais, logo a avaliação ultrassonográfica dessas glândulas deve ser rotina, uma vez que pode auxiliar no diagnóstico de uma dada afecção, principalmente o hiperadrenocorticismo, doença relativamente comum principalmente nos cães, cujo diagnóstico baseia-se na avaliação do eixo hormonal hipotálamo-hipófise-adrenal. Quanto às outras doenças, como neoplasias, cistos, hiperplasias, dentre out...

  14. Importância anatomocirúrgica das características macroscópicas, localização e suprimento vascular das glândulas paratireóides cervicais

    OpenAIRE

    Botelho, João Bosco Lopes; Cançado, Anderson R. S.; Sousa, Elane Araujo de

    2004-01-01

    OBJETIVOS: A importância anatômica e cirúrgica das glândulas paratireóides, notadamente, no curso das tireoidectomias continua viva e despertando interesse científico. Por outro lado, são raros os trabalhos científicos sobre a investigação da anatomia das paratireóides. Dessa forma, o objetivo deste trabalho é contribuir para estabelecer essa ponte anatomocirúrgica. MÉTODO: Estudo macro e microscópico das glândulas paratireóides dissecadas nas peças anatômicas de dezenove cadáveres, todos do ...

  15. Achado incidental de glândula sebácea em colo uterino: provavelmente um processo metaplásico An incidental finding of sebaceous glands in the uterine cervix: a probable metaplastic process

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    Daniel Cury Ogata

    2012-06-01

    Full Text Available As glândulas sebáceas ectópicas são achados muito infrequentes no trato genital feminino. Descrevemos um caso que ocorreu em paciente de 42 anos de idade, submetida à histerectomia total, devido à leiomiomatose e ao prolapso uterino.The ectopic sebaceous glands have been extremely rare findings in female genital system. We describe the case of 42 years-old patient with total hysterectomy due to leiomyomatosis and uterine prolapsed.

  16. Characterization of plasmids in a human clinical strain of Lactococcus garvieae.

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    Mónica Aguado-Urda

    Full Text Available The present work describes the molecular characterization of five circular plasmids found in the human clinical strain Lactococcus garvieae 21881. The plasmids were designated pGL1-pGL5, with molecular sizes of 4,536 bp, 4,572 bp, 12,948 bp, 14,006 bp and 68,798 bp, respectively. Based on detailed sequence analysis, some of these plasmids appear to be mosaics composed of DNA obtained by modular exchange between different species of lactic acid bacteria. Based on sequence data and the derived presence of certain genes and proteins, the plasmid pGL2 appears to replicate via a rolling-circle mechanism, while the other four plasmids appear to belong to the group of lactococcal theta-type replicons. The plasmids pGL1, pGL2 and pGL5 encode putative proteins related with bacteriocin synthesis and bacteriocin secretion and immunity. The plasmid pGL5 harbors genes (txn, orf5 and orf25 encoding proteins that could be considered putative virulence factors. The gene txn encodes a protein with an enzymatic domain corresponding to the family actin-ADP-ribosyltransferases toxins, which are known to play a key role in pathogenesis of a variety of bacterial pathogens. The genes orf5 and orf25 encode two putative surface proteins containing the cell wall-sorting motif LPXTG, with mucin-binding and collagen-binding protein domains, respectively. These proteins could be involved in the adherence of L. garvieae to mucus from the intestine, facilitating further interaction with intestinal epithelial cells and to collagenous tissues such as the collagen-rich heart valves. To our knowledge, this is the first report on the characterization of plasmids in a human clinical strain of this pathogen.

  17. Câmpus Glória: os impactos socioambientais da expansão da Universidade Federal de Uberlândia / Glória Campus: the social and environmental impacts of Federal University of Uberlândia expansion process

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    Marlene Teresinha de Muno Colesanti

    2012-12-01

    Full Text Available A implantação do câmpus Glória da Universidade Federal de Uberlândia é um projeto desenhado pela universidade desde a década de 1970 que somente agora se torna realidade, graças à política de expansão das universidades federais. O projeto do novo câmpus da UFU, discutido com a comunidade universitária e externa entre 2010 e 2011, foi aprovado e teve sua implantação iniciada com a construção dos primeiros prédios. Esse trabalho, fruto de discussões realizadas no Programa de Pós-Graduação em Geografia e desenvolvido no âmbito de um projeto institucional da própria universidade, avalia os efeitos e impactos urbanísticos, ambientais e socioeconômicos da implantação do novo câmpus, a partir dos atuais usos e predominâncias da área que soma quase 5% do perímetro urbano da cidade de Uberlândia e das propostas elaboradas durante os processos do plano diretor físico territorial, do projeto urbanístico e dos estudos de impacto ambiental e urbanístico, discutindo a necessidade e a viabilidade desse grande empreendimento para a cidade e região.

  18. Eletromiografia laríngea em pacientes com disfonia e fechamento glótico incompleto Laryngeal electromyography in dysphonic patients with incomplete glottic closure

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    Noemi Grigoletto De Biase

    2012-12-01

    Full Text Available O diagnóstico de comprometimento do nervo laríngeo superior e de ramos do laríngeo recorrente demanda eletromiografia, pois as alterações à laringoscopia são inespecíficas. OBJETIVO: Avaliar eletrofisiologicamente a função dos nervos laríngeo superior e inferior por meio da atividade elétrica dos músculos por eles inervados, em pacientes com disfonia com coaptação incompleta das pregas vocais à fonação. MÉTODO: Estudo prospectivo; 39 indivíduos com disfonia e fechamento glótico incompleto foram submetidos à eletromiografia dos músculos tireoaritenóideo, cricotireóideo e cricoaritenóideo lateral bilateralmente. Foram avaliadas atividade de inserção, no repouso (fibrilação, onda positiva e fasciculação e durante contração voluntária dos músculos (recrutamento, amplitude e duração do potencial e latência entre início da atividade elétrica e a sonorização. RESULTADOS: Não observamos alteração na atividade de inserção e no repouso. Nenhum paciente apresentou recrutamento alterado. A média da amplitude dos potenciais elétricos esteve compatível com a normalidade nos músculos testados, assim como a duração do potencial e o tempo de latência entre o início da atividade elétrica e a sonorização. CONCLUSÃO: Não observamos sinais de desnervação nos músculos tireoaritenóideo, cricotireóideo e cricoaritenóideo lateral bilateralmente nos pacientes estudados.The lack of specificity in laryngoscopical examination requires that the diagnosis of superior laryngeal and recurrent laryngeal nerve involvement be carried out with the aid of electromyography. OBJECTIVE: This study aims to assess the electrophysiological function of the superior and inferior laryngeal nerves by measuring the electrical activity of the muscles they innervate in dysphonic patients with incomplete closure of the vocal folds during phonation. METHOD: Thirty-nine patients with incomplete glottic closure were enrolled in a

  19. Incisão periauricular para operações da glândula parótida Periauricular incision for surgical procedures on the parotid gland

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    Andy Petroianu

    2010-12-01

    Full Text Available RACIONAL: As incisões mais comuns para parotidectomia consistem em abertura pré ou periauricular prolongadas para a região submandibular ou cervical. Elas podem acompanhar-se de cicatrizes imperfeitas, provocando deformidades cutâneas locais. OBJETIVO: Avaliar o tratamento de afecções cirúrgicas parotídeas através de incisão periauricular apenas. MÉTODO: Foram estudados 39 pacientes consecutivos com moléstias da parótida. Adenoma pleomórfico (20 casos foi a afecção mais encontrada, seguida por outros tumores benignos (9 casos, carcinomas (5 casos, cisto parotídeo (3 casos e parotidite crônica (2 casos. Todas as parotidectomias foram realizadas através de incisão periauricular. Em presença de carcinoma, a linfadenectomia cervical foi conduzida por meio de incisão cervical transversa supra-hióidea homolateral. RESULTADO: A remoção da afecção parotídea foi possível em todos os casos sem incisão cutânea complementar. Todas as cicatrizes tiveram bom resultado estético e, após seis meses, elas estavam quase imperceptíveis. Os pacientes revelaram satisfação com o resultado da operação. Fraqueza facial temporária ocorreu em 28 operações. Desconforto auricular transitório foi registrado em 22 pacientes. Todos tiveram hipoestesia da região operada, que perdurou por até seis meses. As complicações encontradas neste trabalho estão descritas na literatura como esperadas em parotidectomia, independentemente do tipo de incisão. CONCLUSÃO: A incisão periauricular é opção boa e estética para abordagem cirúrgica da glândula parótida.BACKGROUND: The most common incisions for parotidectomy consist of opening or pre periauricular extended to the submandibular or cervical region. They can accompany themselves ragged scars, causing local skin deformities. AIM: The purpose of this study was to evaluate the treatment of parotid surgical diseases using periauricular incision. METHOD: Thirty nine patients with

  20. Contorno de la región glútea: Conceptos actuales y propuesta de clasificación Contouring of gluteal region: Current concepts and proposal for classification

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    G. Avendaño-Valenzuela

    2010-09-01

    Full Text Available La mejora del contorno glúteo y de las regiones paraglúteas es un requerimiento frecuente por parte de las pacientes. Algunos autores han hecho contribuciones múltiples a este tema: cruroplastias, levantamiento glúteo, implantes de glúteo, flancoplastias, liposucción y lipoinyección, técnicas que logran mejoría del contorno glúteo, pero que generalmente requieren una combinación de procedimientos. Sugerimos una clasificación para facilitar la elección del tratamiento quirúrgico más adecuado para la remodelación de la región glútea. Esta clasificación se basa principalmente en la anatomía músculo-esquelética de la región glútea, la cadera y las zonas de deformidad o lipodistrofia. Analizamos un total de 800 mujeres entre 16 y 54 años de edad, a lo largo de un período de 7 años. A todas se les realizó estudio anatómico y fotográfico de la cadera. Analizamos las estructuras músculo-esqueléticas y las zonas con lipodistrofia o deformidad; éstas últimas fueron clasificadas según su frecuencia como zona I, II, III, IV y V. Las deformidades aparecieron con la frecuencia siguiente: tipo I: 5%. Tipo II: 30%. Tipo III: 40%. Tipo IV: 20%. Tipo V: 5%. El propósito de esta nueva clasificación de deformidades de la cadera y su tratamiento es actualizar los conceptos y evolucionar en el tratamiento estético de la cadera con un concepto global. Permite también obtener resultados estéticos más relevantes. La clasificación, aplicable a las mujeres latinas y anglosajonas, ofrece una guía para el estudio preoperatorio de los glúteos y evitar así secuelas tras la cirugía.An improvement in the contouring of gluteal and paragluteal regions has been frequently required by the patients. Some authors have made multiple contributions like cruroplasty, gluteal lift, gluteal implants, flancoplasty, liposuction and lipoinjection, because the improvement of the gluteal contouring requires a combination of procedures. We are

  1. Effects of the cafeteria diet on the salivary glands of trained and sedentary Wistar rats=Efeitos da dieta de cafeteria sobre as glândulas salivares de ratos Wistar sedentários e treinados

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    Sonia Lucy Molinari

    2012-01-01

    Full Text Available The objective of this work was to study the effect of the aerobic physical training and the cafeteria diet introduced after weaning of Wistar rats and on the morphology of the main salivary glands (parotid, submandibular, sublingual. Male rats after weaning were subjected to the cafeteria diet or the standard rodent chow, and either performed aerobic physical training in a treadmill for 100 days, or did not performed any physical activity. Analyses were done considering the response in body weight, adipose tissues and salivary glands, and the data were submitted to statistical treatment (p O objetivo deste trabalho foi avaliar as características morfológicas das glândulas salivares (parótida, submandibular, sublingual apresentadas por ratos submetidos a treinamento físico aeróbio e dieta de cafeteria após o período de lactação. Ratos machos após a lactação consumiram dieta de cafeteria ou ração-padrão para roedores e realizaram treinamento físico aeróbio em esteira rolante por um período de 100 dias, ou não realizaram nenhuma atividade física. Foram feitas análises sobre a resposta do peso corporal, dos tecidos adiposos e das glândulas salivares e os dados submetidos a tratamento estatístico (p < 0,05. A análise morfológica e morfométrica das glândulas salivares foi realizada a partir de cortes histológicos corados com Hematoxilina e Eosina. Apesar do comportamento normofágico, os roedores alimentados com dieta de cafeteria apresentaram maior quantidade de gordura corporal, com repercussão sobre o peso da glândula parótida. A análise morfométrica das glândulas submandibulares indicou redução na proporção dos ácinos serosos como efeito da dieta de cafeteria e do treinamento físico. No entanto, excesso de gordura corporal, dieta de cafeteria e/ou treinamento físico não influenciou a organização histológica das glândulas salivares. Concluiu-se que as glândulas parótidas e submandibulares são mais

  2. Abordagem simplificada do nervo ciático por via posterior, no ponto médio do sulco glúteo-femoral, com uso de neuroestimulador Abordaje simplificado del nervio ciático por vía posterior, en el punto medio del sulco glúteo-femoral, con uso de neuroestimulador Simplified sciatic nerve approach by the posterior route at the median gluteus-femoral sulcus region, with a neurostimulator

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    Neuber Martins Fonseca

    2002-11-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: O bloqueio do nervo ciático pode ser realizado por várias abordagens com vantagens e desvantagens. O nervo ciático é o maior nervo do corpo humano em diâmetro e comprimento. É a continuação do fascículo superior do plexo sacral (L4, L5, S1, S2 e S3. Sai da pelve através do forâmen isquiático maior, passando por baixo do músculo piriforme, desce entre o trocânter maior do fêmur e a tuberosidade isquiática, e ao longo do dorso da coxa, anterior aos músculos bíceps femoral e semitendinoso, até o terço inferior da coxa, onde se divide em dois grandes ramos denominados nervos tibial e fibular comum. Torna-se superficial na borda inferior do músculo glúteo máximo. Baseados nesta descrição anatômica, desenvolvemos uma abordagem posterior, tendo como vantagens a fácil identificação da anatomia de superfície, superficialidade do nervo nesta localização, provocando menor desconforto ao paciente que outras abordagens e podendo utilizar agulha de 5 cm. MÉTODO: Foram estudados 17 pacientes, estado físico ASA I, II ou III, com idades entre 21 e 79 anos, peso de 55 a 90 kg, submetidos a cirurgias em perna ou pé. Após monitorização, o paciente foi posicionado em decúbito ventral e realizado bloqueio no ponto médio do sulco glúteo-femoral (dobra da pele entre a nádega e região posterior da coxa, com auxílio de neuroestimulador, utilizando lidocaína a 1% sem adrenalina (300 mg. Avaliou-se latência, tempo de execução do bloqueio, anestesia dos nervos tibial, fibular comum e cutâneo posterior da coxa. Quando necessário, foi também realizado o bloqueio do nervo safeno com 5 ml de lidocaína a 1%. RESULTADOS: Obteve-se anestesia adequada em todos os casos com o volume e concentração usados. Em nenhum paciente ocorreu anestesia do nervo cutâneo posterior da coxa. O tempo de execução do bloqueio foi de 8,58 ± 5,71 min. A latência foi de 5,88 ± 1,6 min. A duração sensitiva e motora

  3. Conjuntivectomía periglandular: Una nueva alternativa para el tratamiento quirúrgico del prolapso de la glándula del tercer párpado en caninos Periglandular conjunctivectomy: A new alternative to surgical treatment of the prolapsed third eyelid gland in canines

    OpenAIRE

    López, C.; CE Giraldo; JU Carmona

    2011-01-01

    El prolapso de la glándula del tercer párpado se presenta con frecuencia en perros braquiocefálicos, aunque algunas razas meso o dolicocefálicas como Cocker Spaniel y Beagle, entre otras, pueden padecer esta patología. En este artículo se describe una nueva y sencilla técnica quirúrgica para reubicar la glándula del tercer párpado prolapsada mediante la creación de un bolsillo subconjutival acompañado de eliminación parcial del tejido conjuntival que recubre la glándula prolapsada. La ventaja...

  4. Carrot cake with and without gluten: development of formulas and acceptance of product = Bolo de cenoura com e sem glúten: desenvolvimento da formulação e aceitação do produto

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    Angélica Aparecida Maurício

    2012-12-01

    Full Text Available The changes in the processing and the consumer’s growing demand for foods that present, besides the high sensorial and nutritional quality, benefits associated to the health raise the necessity of new ingredients that can assist their needs and also the market’s demand. The current project had as its objective the comparison in theacceptance of different formulations of carrot cake without gluten with wheat germ oil and with gluten and wheat germ oil and palm for consumers; to identify the nutritional composition of these ones as well as the purchase intention. The obtained cakes with the formulations of BIG, BGOM and BGOP, were appraised in their chemicaland physical properties, preference and purchase intention. The cakes with the formula BIG MIX-1 and BGOP 80 g presented chemical properties, physics, preference and purchase intention comparable to the ones of the standard formula. Therefore, MIX-1 and the oil of Palm can be used as ingredients to formulate carrot cake, supplyingproducts with characteristics that assist the demands of the market.= As mudanças no processamento e a crescente exigência do consumidor por alimentos que apresentem, além da alta qualidade sensorial e nutricional, benefícios associados à saúde, fazem surgir à necessidade de novos ingredientes que possam atender a estas exigências do mercado. Objetivou-se com o presente trabalho comparar a aceitação de diferentes formulações de bolo de cenoura sem glúten com óleo de milho e, com glúten e óleo de milho e palma por consumidores; determinar a composição nutricional dos mesmos bem como a intenção decompra. As formulações de bolo de cenoura isentos de glúten (BIG foram compostas por dois mix isentos de glúten (MIX-1 e MIX+1, o bolo de cenoura com glúten e óleo de milho (BGOM considerado como padrão ebolo de cenoura com glúten e óleo de palma (BGOP que foi preparado com duas diferentes proporções de óleo de palma (80 e 100 g. Avaliou-se o

  5. Microalterations in the third eyelid gland of dogs with keratoconjunctivits sicca secondary to distemper Microalterações da glândula terceira pálpebra em cães com cinomose e acometido por ceratoconjuntivite seca

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    F.L.C. Brito

    2007-04-01

    Full Text Available Keratoconjunctivitis sicca is characterized by qualitative or quantitative abnormalities in the aqueous component of the tear film and is frequent in dogs with distemper. Twenty-eight third eyelid glands of end-stage dogs that died from distemper were analyzed by light microscopy. Keratoconjunctivitis sicca was diagnosed based on ophthalmic findings regarding alterations in the ocular surface and by the Schirmer tear test. The excised glands were fixed in 10% buffered formalin and processed for embedding in paraffin. The blocks were cut into 4µm sections and the sections were stained with hematoxylin-eosin and Masson's trichrome. Analysis revealed tubuloacinar gland atrophy, thickening of connective tissue, and mild to intense inflammatory exudation mainly characterized by the presence of plasma cells and histiocytes and by compensatory hypertrophy of some glands. The glandular lumen was hypertrophied and contained shedded epithelial cells, polymorphonuclear cells and red blood cells, in addition to mucus.Analisaram-se, à microscopia óptica, 28 glândulas da terceira pálpebra de cães, que morreram com cinomose. O diagnóstico de ceratoconjuntivite seca baseou-se nas alterações da superfície ocular e no teste da lágrima de Schirmer. À histologia, visibilizaram-se atrofia tubuloacina, espessamento do tecido conjuntivo e exudação inflamatória de leve a intensa com presença de plasmócitos, de histiócitos e por hipertrofia compensatória de algumas glândulas. O lume das glândulas encontrava-se hipertrofiado contendo células epiteliais descamadas, polimorfonucleares e hemácias, além de muco.

  6. Valorização do kiwi (A. deliciosa) de baixo calibre: extração de actinidina e sua aplicação na produção de hidrolisados de glúten

    OpenAIRE

    Henriques, Teresa

    2014-01-01

    O kiwi é o fruto de uma planta pertencente ao género botânico Actinidia, da família das Actinidiaceae. Apesar das suas propriedades bioativas, o desperdício anual associado à venda e processamento do kiwi é bastante significativo. A actinidina é uma cisteína protease que representa 50% da proteína solúvel do kiwi. O objetivo deste trabalho foi valorizar o kiwi e os seus subprodutos estudando o efeito da actinidina no glúten. A enzima foi extraída em quatro condições distintas e os extratos su...

  7. Aspectos clínicos-terapéuticos de la patología de las glándulas salivares en la infección producida por el VIH.

    OpenAIRE

    Malagón Gutiérrez, Sandra Ximena; Chimenos Küstner, Eduardo; López López, José; Jané Salas, Enric; Roselló Llabrés, Xavier; N. Mohammad

    1997-01-01

    La infiltracion de tejido linfocitario es el fenómeno que comparten todas las lesiones glandulares y las linfoadenopatías persistentes asociadas a la infección por el VIH. Aunque existen diferencias histológicas y ultraestructurales precisas entre los diferentes tipos de lesiones en la enfermedad de glándulas salivales asociada al VIH ( EG.S-VIH), todas comparten básicamente las mismas manifestaciones clínicas, con signos tales como xerostomía y adenomegalia. Por lo tanto la actitud del profe...

  8. Perfil diário e os mecanismos de produção de melatonina pela glândula pineal de ratos diabéticos por estreptozotocina.

    OpenAIRE

    Fernanda Gaspar do Amaral

    2009-01-01

    A pineal participa da sincronização do organismo pela síntese de melatonina. Diabetes é um distúrbio metabólico caracterizado por hiperglicemia. Diante da controvérsia sobre a síntese de melatonina em animais diabéticos, esse trabalho objetivou avaliar as alterações da glândula pineal mediante o diabetes induzido por STZ (60mg/kg, i.p.). Ratos wistar (250-280g, 12h/12h claro/escuro) foram utilizados em todos os procedimentos que envolveram técnicas de FACS, microdiálise, HPLC, radiometria da ...

  9. Drama sa muzikom Srećna ruka Arnolda Šenberga kao višemedijski projekt / Drama with music Die Glückliche Hand by Arnold Schoenberg as a multimedia project

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    Mirjana Veselinović-Hofman

    2012-12-01

    Full Text Available Drama with music “The Lucky Hand” (Die Glückliche Hand by Arnold Schoenberg is examined in this study from the perspective of the composer’s concept of specifically combining elements of different arts. The investigation is aimed at the interpretation of Schoenberg’s actual artistic realization of this concept, and, consequently, the type of the breakthrough in the traditional opera genre which was thus effectuated. It concerns a complex lyric stage conception which hints at multimedia forms of modern artistic expression.

  10. Estudo das glândulas mandibulares e ovários de operárias de Apis mellifera L. (Hymenoptera, Apidae mantidas em condiçoes artificiais

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    Claudia Regina de Salvo

    1988-01-01

    Full Text Available Foram estudados os ovários e as glândulas mandibulares de Apis mellifera mantidas em condições artificiais e foi observado que em pequenos grupos (menos de 20 abelhas os órgãos analisados não se desenvolveram.The ovaries and mandibular glands in workers of Apis mellifera under artificial conditions were studied and was observed that in small groups (less than 20 bees the analyzed organs did not develop.

  11. Descripción anatómica de los órganos genitales y glándulas secretoras abdominales del escarabajo Ulomoides dermestoides (fairmare 1893 (Coleoptera:Tenebrionidae

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    Randall E. Chacón Castro

    2009-11-01

    Full Text Available Ulomoides dermestoides es un coleóptero tenebriónido reportado como plaga de granos almacenados, el cual presenta potencial biotecnológico para la bioprospección, al atribuírsele propiedades farmacológicas. Esta investigación se realizó con el objetivo de describir la anatomía de los órganos genitales y las glándulas secretoras abdominales del insecto criado en condiciones de laboratorio (dieta a base de maní, 70% HR, 23°C a 1300 msnm, en el Centro de Investigación en Biotecnología del ITCR, Cartago, Costa Rica. Los órganos genitales y las glándulas abdominales en ambos sexos fueron procesados para la observación macroscópica y para microscopía electrónica de barrido en la Unidad de Investigación en Estructuras Microscópicas de la Universidad de Costa Rica, San José, Costa Rica. En las hembras se observó la mecánica de la estructura ovipositora y se describió sus funciones reproductivas y de defensa, al igual que se encontró dos cercos apicales con acumulaciones sensoriales mecano y quimiorreceptoras. Se reportó estructuras internas esclerotizadas con función en la oviposición, así como las guías que junto con el tejido elástico del oviducto conforman el segmento final del ovipositor. En los machos se identificó el adeago, sus extensiones internas y la forma particular que permite su introducción y acoplamiento durante la cópula, sirviendo también de superficie guía de secreciones defensivas. Las micrografías evidenciaron la presencia de glándulas corrugadas secretoras en ambos sexos (de 1.05 mm de longitud por 350 mm de diámetro, de fuerte adhesión a la pared abdominal por el tejido musculoso de convergencia de los conductos secretores, asociados a la comunicación del gonoporo con el receptáculo glandular. Se encontró células al parecer bacterianas en el interior de las glándulas, esto como posible infección patológica o bien como organismos simbiontes, lo cual no se pudo diferenciar claramente

  12. Carcinosarcoma of the parotid gland featuring foci of malignant giant cell tumor Carcinossarcoma da glândula parótida com áreas de tumor de células gigantes

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    José Castro Geraldes Filho

    2012-04-01

    Full Text Available Salivary gland carcinosarcoma is a rare neoplasm in which both stromal and epithelial components meet the microscopic criteria for malignancy. Seventy-four cases have been reported in English-language literature to date. In this study, we describe the case of an 81-year-old male patient with parotid gland carcinosarcoma featuring foci of undifferentiated carcinoma and sarcomatous components such as chondrosarcoma, high-grade undifferentiated sarcoma, and malignant giant cell tumor. We reviewed and discussed this rare occurrence of salivary gland carcinosarcoma with areas of malignant giant cell tumor, which had been reported only twice previously.O carcinossarcoma de glândulas salivares é uma neoplasia rara na qual os componentes epitelial e estromal apresentam critérios microscópicos de malignidade. Setenta e quatro casos já foram relatados até o momento. Neste relato, descrevemos o caso de um paciente do sexo masculino, 81 anos, com carcinossarcoma da parótida, exibindo áreas de carcinoma indiferenciado e componente sarcomatoso constituído por condrossarcoma, sarcoma de alto grau e tumor de células gigantes. Revisamos e discutimos essa rara observação de carcinossarcoma de glândula salivar exibindo focos de tumor de células gigantes, descrito apenas em dois casos prévios.

  13. Purification, crystallization and preliminary X-ray analysis of the galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) of the ABC transporter from Bifidobacterium longum JCM1217

    Energy Technology Data Exchange (ETDEWEB)

    Wada, Jun [Graduate School of Biostudies, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502 (Japan); Suzuki, Ryuichiro; Fushinobu, Shinya [Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 (Japan); Kitaoka, Motomitsu [National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642 (Japan); Wakagi, Takayoshi; Shoun, Hirofumi [Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 (Japan); Ashida, Hisashi [Graduate School of Biostudies, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502 (Japan); Kumagai, Hidehiko; Katayama, Takane, E-mail: takane@ishikawa-pu.ac.jp [Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, Nonoichi, Ishikawa 921-8836 (Japan); Yamamoto, Kenji [Graduate School of Biostudies, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502 (Japan)

    2007-09-01

    Preliminary X-ray analysis of the galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) of the ABC transporter from B. longum is described. A recombinant galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) from Bifidobacterium longum JCM1217 has been prepared and crystallized by the hanging-drop vapour-diffusion method using 10 mg ml{sup −1} purified enzyme, 0.01 M zinc sulfate, 0.1 M MES buffer pH 5.9–6.4 and 20–22%(v/v) PEG MME 550 in the presence of 5 mM disaccharide ligands. Suitable crystals grew after 10 d incubation at 293 K. The crystals belong to space group C222{sub 1}, with unit-cell parameters a = 106.3, b = 143.6, c = 114.6 Å for the lacto-N-biose I complex and a = 106.4, b = 143.4, c = 115.5 Å for the galacto-N-biose complex, and diffracted to 1.85 and 1.99 Å resolution, respectively.

  14. Enterotoxigenic Escherichia coli (ETEC) adhesin-toxoid MEFA (multiepitope fusion antigen) CFA/I/II/IV-3xSTaN12S-mnLTG192G/L211A derived antibodies inhibit adherence of seven adhesins (CFA/I, CFA/II & CFA/IV), neutralize enterotoxicity of both toxins (LT & STa), and protect piglets against diarrhea.

    Science.gov (United States)

    Nandre, Rahul; Ruan, Xiaosai; Lu, Ti; Duan, Qiangde; Sack, David; Zhang, Weiping

    2017-12-20

    Enterotoxigenic Escherichia coli (ETEC) are a leading cause of children's diarrhea and travelers' diarrhea. Vaccines inducing antibodies to broadly inhibit bacterial adherence and to neutralize toxin enterotoxicity are expected effective against ETEC-associated diarrhea. 6xHis-tagged adhesin-toxoid fusion proteins were shown to induce neutralizing antibodies to several adhesins and LT and STa toxins (X. Ruan, DA Sack, W. Zhang, PLoS ONE, 10:e0121623, 2015). However, antibodies derived from his-tagged CFA/I/II/IV-2xSTaA14Q-dmLT or CFA/I/II/IV-2xSTaN12S-dmLT protein were less effective in neutralizing STa enterotoxicity and were not evaluated in vivo for efficacy against ETEC diarrhea. Additionally, his-tagged proteins are considered less desirable for human vaccines. In this study, we produced a tag-less adhesin-toxoid MEFA (multiepitope fusion antigen) protein, enhanced anti-STa immunogenicity by including a third copy of STa toxoid STaN12S, and examined antigen immunogenicity in a murine model. Moreover, we immunized pregnant pigs with the tag-less adhesin-toxoid MEFA protein and evaluated passive antibody protection against STa+ or LT+ ETEC infection in a pig challenge model. Results showed that tag-less adhesin-toxoid MEFA CFA/I/II/IV-3xSTaN12S-mnLTR192G/L211A induced broad anti-adhesin and antitoxin antibody responses in the intraperitoneally immunized mice and the intramuscularly immunized pigs. Mouse and pig serum antibodies significantly inhibited adherence of seven CFA adhesins (CFA/I, CS1 to CS6) and effectively neutralized both toxins. More importantly, suckling piglets born to the immunized mothers acquired antibodies and were protected against STa+ ETEC and LT+ ETEC diarrhea. These results indicated tag-less CFA/I/II/IV-3xSTaN12S-mnLTR192G/L211A induced broadly protective anti-adhesin and antitoxin antibodies and suggested this adhesin-toxoid MEFA a potential antigen for developing broadly protective ETEC vaccines. Copyright © 2017 American Society for

  15. Gluteus maximus and semitendinosus activation during active prone hip extension exercises Ativação do glúteo máximo e semitendinoso durante exercícios de extensão do quadril em prono

    Directory of Open Access Journals (Sweden)

    ACL Sakamoto

    2009-08-01

    Full Text Available BACKGROUND: Gluteus maximus strengthening exercises are employed in clinical practice as options for the treatment of low back and sacroiliac disorders. However, no studies were found that investigated which were the best exercises to activate this muscle and justify its employment in physical therapy practice. OBJECTIVE: To quantify the electromyographic (EMG activity of the gluteus maximus and semitendinosus muscles during four modalities of therapeutic exercises. METHODS: Thirty-one participants (16 men, 15 women were selected. The EMG activities of the gluteus maximus and semitendinosus was recorded and quantified while the participants performed four modalities of therapeutic exercises, involving active prone hip extension in four positions: knee extension (KE, knee flexion (KF, lateral hip rotation and knee extension (LHRKE, and lateral hip rotation and knee flexion (LHR-KF. RESULTS: Repeated-measures ANOVAs showed that the addition of KF or LHR increased gluteus maximus activity, whereas, KE decreased the activation of this muscle. In contrast, the exercises performed with KE increased semitendinosus activity. CONCLUSIONS: Exercises performed with KF or LHR, or a combination of the two, may be effective choices for gluteus maximus strengthening, however both KF and LHR decreased semitendinosus activity. The exercises performed with KE appeared to be an acceptable choice for semitendinosus activation.CONTEXTUALIZAÇÃO: Exercícios para fortalecimento do glúteo máximo são empregados na prática clínica para tratamento de disfunções da coluna lombar e sacroilíaca. Entretanto, não foram encontrados estudos que investigaram os melhores exercícios para ativar esse músculo de forma a justificar a sua utilização na prática fisioterapêutica. OBJETIVO: Quantificar a atividade eletromiográgica (EMG dos músculos glúteo máximo e semitendinoso durante quatro modalidades de exercícios terapêuticos. MÉTODOS: Trinta e um

  16. Estudo morfofuncional das glândulas mamárias de Mão Pelada, Procyon cancrivorus Morphofunctional study of Crab-eating Raccoon (Procyon cancrivorus mammary gland

    Directory of Open Access Journals (Sweden)

    Tatiana Bellatine

    2010-08-01

    Full Text Available Para a descrição macro e microscópica das glândulas mamárias foram utilizadas três fêmeas de Mão Pelada (Procyon cancrivorus. As amostras das glândulas foram processadas conforme técnicas rotineiras para histologia. As fêmeas estudadas apresentaram 3 pares de glândulas mamárias, sendo um par de glândula mamária abdominal cranial, um par de abdominal caudal e um par de inguinal. As papilas mamárias apresentaram formato pendular, como os canídeos domésticos. Microscopicamente, a glândula mamária apresentou da porção externa para a interna: epiderme (epitélio estratificado pavimentoso queratinizado, derme (tecido conjuntivo frouxo e tecido conjuntivo denso não modelado, fibras musculares lisas e ductos papilíferos que abrem em vários ósteos papilares em formato de "chuveiro". A porção secretora glandular era caracteristicamente túbulo alveolar, com células cuboidais dispostas em camada simples. Os resultados indicam que o conjunto glandular estudado é semelhante ao da cadela (Cannis familiaris tanto em seu aspecto macroscópico quanto em seu aspecto microscópico, este fato sugere que podemos utilizar o Mão Pelada e o Cão como modelos similares de estudo, para identificação de patologias relacionadas a este sistema.Three Procyon cancrivorus females were studied with emphasis for gross and microscopical description of the mammary glands. Samples of the glands were processed with routine techniques for histology. The females studied presented three pairs of mammary glands: one pair of cranial abdominal mammary glands, a second pair of caudal abdominal and a third one, as inguinal mammary glands. Mammary papillae presented a pendulum shape, as in the domestic dogs. Microscopically, the mammary gland consisted from the external to the internal portion (1 of stratified squamous epithelium of the epidermis, (2 dense irregular connective tissue of the derma, and (3 smooth muscle fibers and papillary ducts that flowed

  17. Descrição morfológica e morfométrica da glândula vesicular de cobaias durante o desenvolvimento pós-natal

    Directory of Open Access Journals (Sweden)

    Adriana Gradela

    2013-07-01

    Full Text Available Glândulas vesiculares são essenciais para a reprodução, pois suas secreções afetam a função espermática. Cobaias (Cavia porcellus são um excelente modelo experimental para estudo destas glândulas, contudo não existem dados morfológicos e morfométricos durante seu desenvolvimento. Neste estudo a morfologia (projeções das pregas (PP e altura das células epiteliais (AE da túnica mucosa e a morfometria (massa (MG, volume (VG, comprimento (CG, largura das porções cranial (LCR, média (LM e caudal (LCA das glândulas vesiculares foram determinadas em cobaios (N= 25 com uma (S1, três (S3, cinco (S5, oito (S8 e onze (S11 semanas de idade (N=5/grupo de idade. Em adição massa (MC, comprimento (CC e altura (AC corporais e o índice organo-somático (IOS foram também determinados e o coeficiente de correlação (r estabelecido entre as variáveis. As glândulas restringiam-se a cavidade pélvica e tinham seu interior repleto de uma massa semi-sólida e, após a S8, ocuparam também a cavidade abdominal e tiveram o conteúdo aumentado significativamente. A túnica mucosa era pregueada e forrada de um epitélio simples colunar. MC, CC e AC aumentaram continuamente com a idade. MG, VG, CG e LCA não se alteraram da S1 a S5 e aumentaram na S8; enquanto que MF, VG e LCA também aumentaram na S11. IOS e PP aumentaram na S8 e S11 e AE na S5, S8 e S11. Houve (r significativo entre a idade, Bc e MFg,; IOS e MG na S8 e S11; idade e PP; idade e AE e entre PP e AE. Em conclusão, as glândulas vesiculares de cobaios seguiram o padrão morfológico observado em ratos e hamsters, mas diferiram em alguns aspectos de outros histricomorfos, podendo ser utilizadas como modelo experimental e seu desenvolvimento morfológico e morfométrico podem ser divididos em três fases: da S1 a S5, quando são discretos; da S6 a S8 de idade, quando são acentuados de maneira geral e após a S8, quando há incremento intenso da capacidade secretória.

  18. Actividad citotóxica in Vitro de la mezcla de Annona muricata y Krameria Lappacea sobre células cancerosas de glándula mamaria, pulmón y sistema nervioso central

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    Jorge Arroyo A

    2005-10-01

    Full Text Available Objetivos: Determinar la actividad citotóxica de las fracciones procedentes de la combinación 1:1 del extracto etanólico de hojas de Annona muricata L (guanábana y el extracto acuoso atomizado de la raíz de Krameria lappacea (ratania en cultivos de líneas celulares cancerosas de glándula mamaria (MCF-7, pulmón (H-460 y Sistema nervioso central (SF-268. Materiales y métodos: Para el fraccionamiento de la mezcla 1:1 de Annona mas Krameria se preparó una columna cromatográfica de 50 cm de longitud empleando diclorometano, diclorometano: acetato de etilo y CHCl 3:MeOH como sistemas de elusión de polaridad creciente, obteniéndose 186 fracciones. Se evaluaron las fracciones 2 a 83 en cultivo de células cancerosas de glándula mamaria (MCF-7, de pulmón (H-460 y del sistema Nervioso central (SF-268. Todas las fracciones fueron ensayadas en duplicado. Aquellas fracciones que presenta-ron un porcentaje de crecimiento de células cancerosas (%G <50% en alguna de las tres líneas celulares fueron ensayadas nuevamente a cinco concentraciones, para determinar finalmente la concentración a la cual se inhibe el 50% del crecimiento de las células cancerosas (GI 50. Se consideraron activas aquellas fracciones con una GI 50 <10 µg/mL. Resultados: Las fracciones 7 a 17 procedentes de la asociación de los dos productos naturales frente a los cultivos de las líneas celulares tumorales MCF-7, H-460 y SF-268 mostraron una GI 50 de 1,6, 1,4 y 1,4 µg/mL respectivamente. Conclusiones: Las fracciones 7 a 17 procedentes de la asociación de Annona más Krameria mostraron acción citotóxica in vitro frente al cultivo de células cancerosas de glándula mamaria, pulmón y del sistema nervioso central.

  19. Standing sagittal alignment of the whole axial skeleton with reference to the gravity line in humans.

    Science.gov (United States)

    Hasegawa, Kazuhiro; Okamoto, Masashi; Hatsushikano, Shun; Shimoda, Haruka; Ono, Masatoshi; Homma, Takao; Watanabe, Kei

    2017-05-01

    Human beings stand upright with the chain of balance beginning at the feet, progressing to the lower limbs (ankles, knees, hip joints, pelvis), each of the spinal segments, and then ending at the cranium to achieve horizontal gaze and balance using minimum muscle activity. The details of the alignment and balance of the chain, however, are not clearly understood, due to the lack of information regarding the three-dimensional (3D) orientation of all bony elements in relation to the gravity line (GL). We performed a clinical study to clarify the standing sagittal alignment of whole axial skeletons in reference to the GL using the EOS slot-scanning 3D X-ray imaging system with simultaneous force plate measurement in a healthy human population. The GL was defined as a vertical line drawn through the centre of vertical pressure measured by the force plate. The present study yielded a complete set of physiological alignment measurements of the standing axial skeleton from the database of 136 healthy subjects (a mean age of 39.7 years, 20-69 years; men: 40, women: 96). The mean offset of centre of the acoustic meati from the GL was 0.0 cm. The offset of the cervical and thoracic vertebrae was posterior to the GL with the apex of thoracic kyphosis at T7, 5.0 cm posterior to the GL. The sagittal alignment changed to lordosis at the level of L2. The apex of the lumbar lordosis was L4, 0.6 cm anterior to the GL, and the centre of the base of the sacrum (CBS) was just posterior to the GL. The hip axis (HA) was 1.4 cm anterior to the GL. The knee joint was 2.4 cm posterior and the ankle joint was 4.8 cm posterior to the GL. L4-, L5- and the CBS-offset in subjects in the age decades of 40s, 50s and 60s were significantly posterior to those of subjects in their 20s. The L5- and CBS-offset in subjects in their 50s and 60s were also significantly posterior to those in subjects in their 30s. HA was never posterior to the GL. In the global alignment, there was a positive correlation

  20. Bloqueio do nervo isquiático por abordagem posterior simplificada no ponto médio do sulco glúteo-femoral: estudo com diferentes volumes de lidocaína a 1%

    OpenAIRE

    Neuber Martins Fonseca; Beatriz Lemos Mandim; Roberto Araújo Ruzi; Fabiana Rosa Tavares

    2006-01-01

    JUSTIFICATIVA E OBJETIVOS: O bloqueio do nervo isquiático por via subglútea foi descrito com sucesso em estudo anterior, sendo mais uma opção entre as várias abordagens possíveis. O nervo isquiático torna-se superficial na borda inferior do músculo glúteo máximo, permitindo seu acesso com fácil localização, pouco desconforto e baixo risco de punção acidental de grandes vasos. O objetivo deste estudo foi avaliar o bloqueio do nervo isquiático por esta abordagem simplificada com diferentes volu...

  1. Análise videolaringoestroboscópica de pacientes submetidos à radioterapia para tratamento de câncer glótico Videolaryngostroboscopic analysis of patients submitted to radiation therapy for the treatment of glottic cancer

    Directory of Open Access Journals (Sweden)

    André Luís Quarteiro

    2010-02-01

    Full Text Available Sendo a radioterapia oncologicamente adequada no tratamento do câncer precoce da laringe, é importante estudar o padrão vibratório, que é componente crítico para os resultados vocais. OBJETIVO: Analisar os achados videolaringoestroboscópicos em um grupo de pacientes submetidos à radioterapia para o tratamento do câncer glótico precoce. MÉTODOS: Estudo retrospectivo realizado através da avaliação de 20 pacientes estadiados como T1a glótico de 1995 a 2005. Um protocolo videolaringoestroboscópico foi aplicado. RESULTADOS: O fechamento glótico foi completo em 17 pacientes. A amplitude foi normal em 14 pregas vocais tratadas e em 18 pregas contralaterais. O padrão da onda vibratória mucosa foi sempre totalmente presente, normal ou discretamente diminuído em ambas as pregas vocais, com melhores resultados para a prega vocal contralateral em comparação com aqueles verificados nas pregas vocais com o tumor. A periodicidade foi sempre regular em todos os casos. A simetria e os movimentos das pregas vestibulares e das aritenoides foram normais. Houve constrição supraglótica lateral ocasional em quatro casos. O aspecto da mucosa foi edematoso em seis pacientes. Formação de bola de muco foi observada em 12 pacientes. CONCLUSÕES: O padrão vibratório foi normal ou discretamente diminuído para ambas as pregas vocais após tratamento radioterápico para câncer glótico T1a.Radiation therapy is an adequate treatment for early laryngeal cancer, and it is important to study the vocal fold vibratory pattern, which is essential for a favorable voice outcome. AIM: To analyze laryngostroboscopic findings in a group of patients who underwent radiation therapy for the treatment of early glottic cancer. METHODS: A retrospective study was conducted in order to evaluate 20 patients staged as T1a glottic tumors in the period from 1995 to 2005. A laryngostroboscopic protocol was applied. RESULTS: Glottic closure was complete in 17 patients

  2. Imunidade inata da glândula mamária bovina: resposta à infecção Innate immunity of the bovine mammary gland: response to infection

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    Deolinda Maria Vieira Filha Carneiro

    2009-09-01

    Full Text Available A imunidade na glândula mamária pode ser classificada, assim como em outros sistemas, em inata ou inespecífica e adaptativa ou específica. A imunidade inata é a defesa predominante durante os estágios iniciais da infecção. As respostas inespecíficas estão presentes no local da infecção ou são ativadas rapidamente por numerosos estímulos e não aumentam pela exposição repetida ao mesmo agente etiológico. O primeiro obstáculo enfrentado por um patógeno para adentrar o úbere é composto pela barreira formada pelo esfíncter do teto e pelo tampão de queratina formado pelo epitélio queratinizado. Uma vez que o microrganismo tenha atravessado o canal do teto e alcançado a cisterna mamária, passam a atuar diversos fatores solúveis e celulares. Dentre os fatores solúveis, estão presentes: lactoperoxidase, sistema complemento, citocinas, lactoferrina, lisozima e NAGase. As defesas celulares inespecíficas na glândula mamária são representadas pelos neutrófilos, pelos macrófagos e pelas células natural killer. Na medida em que esses mecanismos funcionam adequadamente, a maioria dos patógenos será rapidamente eliminada antes que o sistema imune específico seja ativado, sem resultar em alterações na quantidade ou qualidade do leite produzido. Uma melhor compreensão sobre os mecanismos de defesa da glândula mamária e suas alterações durante os períodos críticos da infecção é imprescindível para o desenvolvimento de métodos mais eficazes de profilaxia e controle da mastite, a principal doença dos ruminantes leiteiros. O presente estudo revisou os principais aspectos responsáveis pelo desenvolvimento da imunidade inata na glândula mamária bovina.The immunity in the mammary gland can be, as in other systems, classified in innate or adaptive immunity. The innate immunity is the predominant defense during the initial periods of infection. The non-specifics answers are present or are quickly activated in the

  3. Actividad citotóxica in vitro de la mexcla de Annona muricata y Krameria lappacea sobre células cancerosas de glándula mamaria, pulmón y sistema nervioso central

    OpenAIRE

    Jorge Arroyo A; Mahabir Prashad G; Yelkaira Vásquez B; Elena Li P; Gloria Tomás C

    2005-01-01

    Objetivos: Determinar la actividad citotóxica de las fracciones procedentes de la combinación 1:1 del extracto etanólico de hojas de Annona muricata L (guanábana) y el extracto acuoso atomizado de la raíz de Krameria lappacea (ratania) en cultivos de líneas celulares cancerosas de glándula mamaria (MCF-7), pulmón (H-460) y sistema nervioso central (SF-268). Materiales y métodos: Para el fraccionamiento de la mezcla 1:1 de Annona mas Krameria se preparó una columna cromatográfica de 50 cm de l...

  4. Identificación y caracterización de centros de unión para purinas de membrana plasmática de células aisladas de glándulas gástricas

    OpenAIRE

    Asurabarrena Iraola, Carmen

    2014-01-01

    149 p. : il. Se ha diseñado un método para aislar y separar las fracciones apical y baso lateral de la membrana plasmática de las células parietales de las glándulas gástricas de conejo y se ha caracterizado su grado de purificación mediante el estudio de las actividades de conocidos enzimas marcadores de cada fracción. En los estudios de unión del radioligando [35S]dATP?S a dichas fracciones de membrana se ha comprobado que existe una alta densidad de sitios de unión esp...

  5. Regular consumption of a complementary food fortified with ascorbic acid and ferrous fumarate or ferric pyrophosphate is as useful as ferrous sulfate in maintaining hemoglobin concentrations >105 g/L in young Bangladeshi children.

    Science.gov (United States)

    Davidsson, Lena; Sarker, Shafiqual Alam; Jamil, Kazi Asif; Sultana, Shamima; Hurrell, Richard

    2009-06-01

    Non-water-soluble iron compounds have been reported to be less well absorbed than ferrous sulfate in young children, and concern has been raised about their usefulness as food fortificants. The objective was to evaluate the usefulness of ferrous fumarate and ferric pyrophosphate, compared with ferrous sulfate, in maintaining hemoglobin concentrations >105 g/L in Bangladeshi children. Two hundred thirty-five children aged 7-24 mo (hemoglobin >105 g/L) were randomly assigned in a double-blind study to receive an infant cereal fortified with ferrous fumarate, ferric pyrophosphate, or ferrous sulfate. One serving of cereal (9.3 mg Fe; molar ratio of ascorbic acid to iron of 3:1) was consumed per day, 6 d/wk, for 9 mo. Blood samples were drawn at 4.5 and 9 mo. Raw data were reformatted, and a "time to event" was calculated that corresponded to reaching the following thresholds: hemoglobin 10 mg/L at baseline, 4.5 mo, or 9 mo. Data were censored when children did not reach the threshold or were lost to follow-up. A Kaplan-Meier approach was used to compare the 3 groups. No statistically significant differences were observed for hemoglobin 10 mg/L (P = 0.508). Contrary to earlier concerns, these results do not indicate differences in usefulness between water-soluble and non-water-soluble iron compounds in maintaining hemoglobin concentrations and preventing iron deficiency. These data will be important in the development of food-fortification strategies to combat anemia and iron deficiency in highly vulnerable population groups.

  6. Type 1 Immune Mechanisms Driven by the Response to Infection with Attenuated Rabies Virus Result in Changes in the Immune Bias of the Tumor Microenvironment and Necrosis of Mouse GL261 Brain Tumors.

    Science.gov (United States)

    Bongiorno, Emily K; Garcia, Samantha A; Sauma, Sami; Hooper, D Craig

    2017-06-01

    Immunotherapeutic strategies for malignant glioma have to overcome the immunomodulatory activities of M2 monocytes that appear in the circulation and as tumor-associated macrophages (TAMs). M2 cell products contribute to the growth-promoting attributes of the tumor microenvironment (TME) and bias immunity toward type 2, away from the type 1 mechanisms with antitumor properties. To drive type 1 immunity in CNS tissues, we infected GL261 tumor-bearing mice with attenuated rabies virus (RABV). These neurotropic viruses spread to CNS tissues trans-axonally, where they induce a strong type 1 immune response that involves Th1, CD8, and B cell entry across the blood-brain barrier and virus clearance in the absence of overt sequelae. Intranasal infection with attenuated RABV prolonged the survival of mice bearing established GL261 brain tumors. Despite the failure of virus spread to the tumor, infection resulted in significantly enhanced tumor necrosis, extensive CD4 T cell accumulation, and high levels of the proinflammatory factors IFN-γ, TNF-α, and inducible NO synthase in the TME merely 4 d postinfection, before significant virus spread or the appearance of RABV-specific immune mechanisms in CNS tissues. Although the majority of infiltrating CD4 cells appeared functionally inactive, the proinflammatory changes in the TME later resulted in the loss of accumulating M2 and increased M1 TAMs. Mice deficient in the Th1 transcription factor T-bet did not gain any survival advantage from RABV infection, exhibiting only limited tumor necrosis and no change in TME cytokines or TAM phenotype and highlighting the importance of type 1 mechanisms in this process. Copyright © 2017 by The American Association of Immunologists, Inc.

  7. Bartholin's gland cysts: management with carbon-dioxide laser vaporization Cistos da glândula de Bartholin: tratamento com vaporização laser com CO2

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    Ana Cristina Neves Figueiredo

    2012-12-01

    Full Text Available PURPOSE: To evaluate the effectiveness, recurrence rate, and complications of carbon-dioxide laser vaporization in the treatment of Bartholin's gland cysts. METHODS: A retrospective study including 127 patients with symptomatic Bartholin' gland cysts submitted to carbon-dioxide laser vaporization at our institution from January 2005 to June 2011. Patients with Bartholin's gland abscesses and those suspected of having neoplasia were excluded. All procedures were performed in an outpatient setting under local anaesthesia. Clinical records were reviewed for demographic characteristics, anatomic parameters, intraoperative and postoperative complications, and follow-up data. Data were stored and analyzed in Microsoft Excel® 2007 software. A descriptive statistical analysis was performed, and its results were expressed as frequency (percentage or mean±standard deviation. Complication, recurrence, and cure rates were calculated. RESULTS: The mean age of the patients was 37.3±9.5 years-old (range from 18 to 61 years-old. Seventy percent (n=85 of them were multiparous. The most common symptom was pain and 47.2% (n=60 of patients had a history of previous medical and/or surgical treatment for Bartholin's gland abscesses. Mean cyst size was 2.7±0.9 cm. There were three (2.4% cases of minor intraoperative bleeding. Overall, there were 17 (13.4% recurrences within a mean of 14.6 months (range from 1 to 56 months: ten Bartholin's gland abscesses and seven recurrent cysts requiring reintervention. The cure rate after single laser treatment was 86.6%. Among the five patients with recurrent disease that had a second laser procedure, the cure rate was 100%. CONCLUSIONS: At this institution, carbon-dioxide laser vaporization seems to be a safe and effective procedure for the treatment of Bartholin's gland cysts.OBJETIVO: Avaliar a eficácia, a taxa de recorrência e as complicações da vaporização laser com CO2 no tratamento dos cistos da glândula de

  8. Using human urine as food for cyanobacteria in LSS

    Science.gov (United States)

    Kalacheva, Galina; Gribovskaya, Iliada; Kolmakova, Angela

    In biological LSS: human, higher plants, algae, united by common cycle of matter, native human urine is the most problematic substance for using in inter-link exchange. It contains urea, ammonium compounds and up to 10 g/l of NaCl. Each of the mentioned components is toxic for growing higher plants. As for inferior plants, experiments showed that cyanobacteria of genus Spirulina platensis and similar genus Oscillatoria deflexa can grow at NaCl concentrations up to 20 g/l and NH4Cl concentrations up to 800 mg/l. These cyanobacteria can be used in LSS as a photosynthesizing link. Besides, S. platensis is edible for humans and fish. To use urine as food for algae, it is necessary to remove urea and organics. All previously used methods for urine treatment aimed at urea destruction: heating to 300oC, ultraviolet exposure, freezing, oxidation on reactor with hydrogen peroxide, had no effect. We used the following method of urine treatment: urine evaporation till dry residue, subsequent combustion in muffle furnace at 450-500oC and creation of ash water extract of the same volume as the initial urine. Comparison of standard Zarrouk's solution for S. platensis and O. deflexa with the water extract of urine ash showed that the concentrations of K, Ca, Mg, P, S were similar. Successful experiments were made with O. deflexa that were grown on nutrient solution made of the water extract of urine ash with 10 g/l of NaHCO3 and 2 g/l of NaNO3. The sources of intersystem production of HCO3 and NO3 were shown, and the biochemical composition of the investigated algae species, including mineral composition, protein, carbohydrate, amino acid, lipid and vitamin content were studied.

  9. Variação no número de glândulas e produção de óleo em flores de Stigmaphyllon paralias A. Juss. (Malpighiaceae Variation in the number of glands and oil production in flowers of Stigmaphyllon paralias A. Juss. (Malpighiaceae

    Directory of Open Access Journals (Sweden)

    Paula Dib de Carvalho

    2005-06-01

    Full Text Available Foi estudada uma população de Stigmaphyllon paralias (Malpighiaceae em Feira de Santana, Bahia, Brasil, visando analisar a variação do número de glândulas e sua produção de óleo nas flores. Dos indivíduos analisados, 76% não variaram o número de glândulas entre suas flores, ocorrendo 41% de indivíduos com flores que possuem 10 glândulas, 31% com oito glândulas e 4% com nove glândulas. Nas flores dos morfos que apresentam oito e nove glândulas, as glândulas ausentes são sempre as da sépala inferior. Centris leprieuri e Epicharis sp. (Apidae: Centridini foram as únicas espécies que visitaram S. paralias, coletando óleo como recompensa. A sépala inferior localiza-se sob o abdome do polinizador quando pousado na flor, tornando-se inacessível à coleta de óleo. Sugerimos que a incapacidade de utilização de algumas glândulas pelos polinizadores possa ter possibilitado o aparecimento de morfos nas populações que não apresentam estas glândulas como caráter adaptativo por gerar economia de recursos na produção de recompensa não utilizada pelo polinizador. Todas as glândulas de todos os morfos são funcionais. As flores dos dois morfos principais com oito e 10 glândulas, produzem a mesma quantidade total de óleo. As glândulas das flores com oito glândulas aumentam a produção em cerca de 20%, compensando a produção total de óleo por flor.A population of Stigmaphyllon paralias (Malpighiaceae was studied in Feira de Santana, Bahia, Brazil. Variation of the number of oil glands and oil production in the flowers were analized. 76% of the individuals did not present variation on the number of glands among their flowers, 41% presented flowers with 10 glands, 31% with eight glands and 4% with nine glands. In the flowers of the morphs with eight or nine glands, the absent glands were always associated to the inferior sepal. Only Centris leprieuri and Epicharis sp. (Apidae: Centridini visited flowers of S. paralias

  10. Comparação da ativação mioelétrica do glúteo máximo e bíceps femoral entre os agachamentos paralelo e com passada à frente Comparación de la activación mioeléctrica de los glúteos y bíceps femoral entre las sentadillas con los pies paralelos y los pies uno frente al otro Comparison of myoelectric activity of gluteus maximus and biceps femoris between parallel and lunge squat

    Directory of Open Access Journals (Sweden)

    Gustavo Leporace

    2012-09-01

    Full Text Available O objetivo desse estudo foi comparar a atividade mioelétrica (EMG do glúteo máximo e bíceps femoral entre o agachamento com passada à frente e com pés paralelos. Sete indivíduos realizaram oito repetições do agachamento paralelo (AP e com passada à frente (AF deslocando sobrecarga relativa a 50% da massa corporal. O EMG do glúteo máximo e bíceps femoral foi capturado, filtrado por um filtro passa-banda Butterworth de 4ª ordem (20-400 Hz e obtido valores RMS. Utilizou-se o teste de Wilcoxon para comparar o EMG normalizado entre os dois exercícios. Tanto o bíceps femoral (p = 0,041 quanto o glúteo máximo (p = 0,0059 apresentaram maior ativação no AF (30,9% para glúteo; 42,6% para bíceps em comparação ao AP (21,2% para glúteo; 24,7% para bíceps. Apesar da ativação moderada durante a execução dos exercícios, girando em torno de 25% a 45%, a resposta foi maior, para os participantes do estudo, no AF.El objetivo de este estudio fue comparar la actividad mioeléctrica (EMG del músculo glúteo mayor y el bíceps femoral entre la sentadilla con los pies uno frente al otro y los pies paralelos. Siete sujetos realizaron ocho repeticiones de la sentadilla paralela (AP y con los pies uno frente al otro (FA desplazando una masa igual a 50% del peso corporal. El EMG del músculo glúteo mayor y el bíceps femoral fueron capturados, filtrada por un filtro de banda de 4 º orden Butterworth (20-400 Hz y los valores RMS obtenidos. Se utilizó el test de Wilcoxon para comparar la EMG normalizada entre los dos ejercicios. Tanto el bíceps femoral (p = 0,041 y el glúteo mayor (p = 0,0059 mostraron una mayor activación en la FA (30,9% para lo glúteo y 52% para lo bíceps en comparación con el AP (21,2% para lo glúteo y 24,7% para lo bíceps. Aunque la activación moderada durante la ejecución de los ejercicios fue moderado, situándose en torno a un 25% a 45%, la respuesta fue mayor en el FA para los participantes del estudio

  11. Influence of various bile acids on the metabolism of glycyrrhizin and glycyrrhetic acid by Ruminococcus sp. PO1-3 of human intestinal bacteria.

    Science.gov (United States)

    Akao, T

    1999-08-01

    Ruminococcus sp. PO1-3, an intestinal bacterium isolated from human feces, metabolized glycyrrhizin (GL) to glycyrrhetic acid (GA) and GA to 3-oxo-glycyrrhetic acid (3-oxo-GA) and possessed GL beta-D-glucuronidase and 3beta-hydroxysteroid dehydrogenase (3beta-HSD) involved in the metabolism of GL. This bacterial growth was enhanced by GL at a concentration of 0.4 mm and was suppressed by GA at concentration of 1.0 mM. Chenodeoxycholic acid, deoxycholic acid and lithocholic acid among the bile acids added to this bacterium suppressed the growth and GL beta-D-glucuronidase activity and 3beta-HSD activity incident to it at a concentration of 1.0 mM, while cholic acid, hyodeoxycholic acid and glycine and taurin conjugates of cholic acid, chenodeoxycholic acid, deoxycholic acid and lithocholic acid had almost no effect on this bacterium at a concentration of 0.2 to 1.0 mm. However, these enzyme activities of this sonicated bacteria were inhibited by all of these bile acids. Although each bile acid and GL added to bacteria at the same time suppressed the growth and the amount of metabolite GA by all bile acids used except cholic acid, taurocholic acid and taurodeoxycholic acid with GL, a combination of each bile acid and GA eased the growth inhibition caused by GA at a concentration of 0.2 mM and enhanced the amount of metabolite 3-oxo-GA by the glycine conjugate of bile acids with GA. GL or GA added after 6 h culture with each of these bile acids and bacteria was metabolized to a relatively large amount of GA by chenodeoxycholic acid and lithocholic acid and their glycine and taurine conjugates, glycocholic acid and taurodeoxycholic acid, or had almost no effect on the amount of metabolite 3-oxo-GA, respectively. These results showed that although GL added after the exposure to bile acid and GA and bile acid added at the same time as bacteria had different bile acid action, these conditions enhanced the amount of metabolite GA from GL and metabolite 3-oxo-GA from GA.

  12. A prospective randomized study comparing two commercially available types of human embryo culture media: G1-PLUS™/G2-PLUS™ sequential medium (Vitrolife) and the GL BLAST™ sole medium (Ingamed).

    Science.gov (United States)

    Ceschin, Ianae I; Ribas, Mariana H; Ceschin, Alvaro P; Nishikawa, Lucileine; Rocha, Claudia C; Pic-Taylor, Aline; Baroneza, José Eduardo

    2016-03-01

    To check the efficacy of two types of commercially available embryo culture medium: G1-PLUS™/G2-PLUS™ sequential (Vitrolife, Gothenburg, Sweden) and GV BLAST™ sole (Ingamed, Maringá, Brazil) with regards to fertilization, cleavage, blastocyst and pregnancy rates. Prospective and randomized study conducted from March to July 2015, using the medical records of 60 patients submitted to Intracytoplasmic Sperm Injection techniques (ICSI). Data regarding the age of patients, together with fertilization, cleavage, blastocyst and pregnancy rates, were collected and compared in relation to the: G1-PLUS™/G2-PLUS™ sequential and GV BLAST™ sole mediums. The data were tabulated and compared using the Pearson's Chi-Square test (95% CI). There was no significant difference when comparing patients divided into higher and lower fertility age. No significant statistical difference was noted between the fertilization rates (P=0.59), cleavage (P=0.91), evolution to blastocyst (P=0.33) and total pregnancy (P=0.83) when comparing the embryos cultured in the different media analysed. We conclude that the G1-PLUS™/G2-PLUS™ sequential and GV BLAST™ sole mediums are equally effective with regards to fertilization, cleavage, blastocyst development and total pregnancy rates.

  13. [Effects of union of albumin and egg yolk on human semen cryopreservation].

    Science.gov (United States)

    Huang, Dong-Hui; Zhao, Hu; Xiong, Cheng-Liang; Sheng, Ji-Yun; Zhou, Jie-Ling

    2006-02-01

    To provide a more effective cryoprotective medium (CPM), effect of union of albumin and egg yolk on human sperm cryopreservation was studied. Egg yolk-glycerol-sodium citrate was regarded as CPM of the control group and egg yolk-glycerol-sodium citrate with different concentrations of albumin (1, 2, 3, 4, 5 g/L) were regarded as CPMs of experiment groups. Before and after cryopreservation, sperm movement parameters were assessed by using computer-aided sperm analyzer (CASA) system, and then egg yolk-glycerol-sodium citrate group added 1 g/L albumin was selected, whose movement parameters were the best among the experimental groups, and egg yolk-glycerol-sodium citrateto group as the control to compare sperm survival rate, membrane integrity, function of mitochondrion and ultrastruction. Sperm in egg yolk-glycerol-sodium citrate added I g/L albumin showed significantly higher motility, viability than those in the control group and other experimental groups (P egg yolk-glycerol-sodium citrate group added 1 g/L albumin had significantly higher survival rate, head unpigmenting rate than those in control group (P egg yolk-glycerol-sodium citrate group added I g/L albumin manifested significantly higher succinate dehydrogenase (SDH) activity than that in control group (P albumin and egg yolk has better effects on human sperm cryopreservation than those of solitary use of egg yolk. The action of albumin is related to its concentration, and albumin combining with egg yolk may have plus and complementary effects on human sperm cryopreservation.

  14. Colgajo miocutáneo de glúteo mayor en isla con cierre V en Y para la cobertura de úlceras isquiáticas Island miocutaneous gluteus maximus flap for coverage of ischiatic ulcers

    Directory of Open Access Journals (Sweden)

    S. Llanos Olmedo

    2006-03-01

    Full Text Available Las úlceras en la región isquiáticas ocurren por la permanencia en posición de sentado durante períodos prolongados. Son las úlceras con la mayor recidiva y presencia de lechos ulcerosos múltiples y sinuosos. Esto ocasiona hospitalización prolongada, aseos quirúrgicos repetidos, antibioterapia múltiple y retraso de la rehabilitación. La cobertura se puede realizar con colgajos miocutáneos de glúteo mayor, bíceps femoris, gracilis, tensor de fascia lata, semimembranoso y semitendinoso, entre otros. El colgajo miocutáneo de glúteo mayor realizado para la cobertura de úlceras isquiáticas, es actualizado en este trabajo y se incorpora como variante la utilización de una isla cutánea irrigada por perforantes, que avanza al lecho con cierre V en Y sin tensión. Nuestro objetivo es demostrar que el colgajo de glúteo mayor sería de primera elección en la cobertura de úlceras isquiáticas. Proponemos como variante quirúrgica la liberación completa del músculo, proveyendo un amplio eje de rotación, y una isla de piel sobre él, produciendo un cierre sin tensión. Todo esto reduciría la morbilidad y recidiva. Se realizaron 13 colgajos miocutáneos de glúteo mayor de avance y rotación con cierre V en Y para la cobertura de úlceras isquiáticas de presión grado III y IV. Las patologías asociadas fueron tetraplejia y paraplejia por lesión medular y paraplejia espástica familiar. El seguimiento fue de 6 meses hasta 7 años. Hasta la fecha no se ha documentado morbilidad ni recidiva. En nuestra experiencia el colgajo miocutáneo de glúteo mayor seria de elección para cierre de úlceras isquiáticas por su volumen de relleno e importante irrigación. El cierre sin tensión estaría dado por la liberación completa del músculo produciendo un amplio eje de rotación, y por una isla de piel que se transporta sobre élThe ischiatic ulcers develop as a consecuense of long sitting position. This kind of ulcers relapse in a great

  15. Absolute Quantification of Human Milk Caseins and the Whey/Casein Ratio during the First Year of Lactation.

    Science.gov (United States)

    Liao, Yalin; Weber, Darren; Xu, Wei; Durbin-Johnson, Blythe P; Phinney, Brett S; Lönnerdal, Bo

    2017-10-09

    Whey proteins and caseins in breast milk provide bioactivities and also have different amino acid composition. Accurate determination of these two major protein classes provides a better understanding of human milk composition and function, and further aids in developing improved infant formulas based on bovine whey proteins and caseins. In this study, we implemented a LC-MS/MS quantitative analysis based on iBAQ label-free quantitation, to estimate absolute concentrations of α-casein, β-casein, and κ-casein in human milk samples (n = 88) collected between day 1 and day 360 postpartum. Total protein concentration ranged from 2.03 to 17.52 with a mean of 9.37 ± 3.65 g/L. Casein subunits ranged from 0.04 to 1.68 g/L (α-), 0.04 to 4.42 g/L (β-), and 0.10 to 1.72 g/L (α-), with β-casein having the highest average concentration among the three subunits. Calculated whey/casein ratio ranged from 45:55 to 97:3. Linear regression analyses show significant decreases in total protein, β-casein, κ-casein, total casein, and a significant increase of whey/casein ratio during the course of lactation. Our study presents a novel and accurate quantitative analysis of human milk casein content, demonstrating a lower casein content than earlier believed, which has implications for improved infants formulas.

  16. Avaliação dos rótulos de diferentes marcas de pães integrais versus pães sem glúten comercializados no Município de Vitória da Conquista – BA

    OpenAIRE

    Cabral Ferraz, Elisangela; FACULDADE INDEPENDENTE DO NORDESTE - FAINOR; Moreira Cruz, Maisa; Faculdade Independente do Nordeste; do Carmo Freitas, Erlania; Universidade Estadual do Sudoeste da Bahia - UESB

    2016-01-01

    Na busca constante por uma alimentação saudável, muitas pessoas preferem consumir pães sem glúten ao invés dos tradicionais, por influencias da mídia que os convence que esses contenham em sua preparação mais vitaminas, minerais e fibras e leva ao emagrecimento. Existe ainda um número de pessoas que são obrigadas a substituir esses pães por pães sem glúten, por serem portadores da doença celíaca que é uma doença autoimune desencadeada pela ingestão de cereais que contem glúten. O presente tra...

  17. Histometría de la glándula sublingual de ratones (Mus musculus machos y hembras infectados con la cepa RAL del parásito de Chagas, Trypanosoma cruzi

    Directory of Open Access Journals (Sweden)

    Sérgio de Albuquerque

    2008-06-01

    Full Text Available Analizamos morfológica e histométricamente la glándula sublingual de ratones infectados con la cepa RAL del Trypanosoma cruzi, en machos y hembras. Usamos ratones albinos (Mus musculus, variedad Swiss, inoculados con 2x104 tripomastigotes sanguíneos de la cepa RAL del T. cruzi.. Sacrificamos los animales en el pico de la parasitemia (12º día y fijamos las glándulas sublinguales en ALFAC. Los cortes histológicos teñidos con HE fueron evaluados histométricamente (cariometría y estereología. La parasitemia fue más elevada en las hembras. Histopatológicamente, los "ácinos" (acini de los animales infectados eran menores, con escasa secreción, y conductos estriados menores. Los núcleos de las "medialunas" eran menores y había nidos de amastigotes en el citoplasma. Cariométricamente, los núcleos de los ácinos, medialunas y conductos estriados eran menores en los ratones infectados. Estereológicamente, los volúmenes relativos ocupados por ácinos y conductos estriados fueron menores e, inversamente, fue mayor el volumen para el tejido conjuntivo de los machos infectados. Las densidades de superficie de ácinos y conductos fueron mayores, y el diámetro y el espesor de la pared menores, en este grupo. Por otro lado, la densidad de ácinos fue menor, y las de los conductos estriados y tejido conjuntivo, fueron mayores en las hembras infectadas. Las densidades de superficie de ácinos y conductos estriados fueron mayores, mientras que el diámetro y espesor de la pared de los ácinos fueron menores (y las de los conductos estriados mayores, en este grupo. La cepa RAL del T. cruzi causó un cuadro general de atrofia general en la glándula sublingual, con numerosos nidos de parásitos.Histometry of the sublingual gland in male and female mice (Mus musculus infected with the RAL strain of the Chagas parasite, Trypanosoma cruzi. The aim of this work was to analyze histologically and histometrically the sublingual gland of mice infected

  18. Efeito imediato da técnica de firmeza glótica nas laringectomias parciais horizontais supracricoides: estudo inicial Hands-over-mouth exercise in supracricoid horizontal partial laryngectomy: initial study

    Directory of Open Access Journals (Sweden)

    Marina Lang Fouquet

    2012-01-01

    Full Text Available A laringectomia parcial horizontal supracricoidea é indicada para o tratamento do câncer da laringe que não invade a subglote e preserva ao menos uma das aritenoides. Esta cirurgia possibilita a manutenção das funções respiratória e esfinctérica da laringe e permite a fonação com característica predominantemente rugosa. O trabalho fonoaudiológico visa promover a aproximação das estruturas remanescentes para reabilitar a deglutição e fonação do paciente. A técnica de firmeza glótica é um exercício indicado para melhorar a coaptação das pregas vocais, sendo realizado por meio da oclusão quase total da boca com a mão em concha, durante a emissão de fricativo sonoro sustentado. Para compreender como esta técnica atua nas estruturas supraglóticas, foi avaliada a configuração laríngea e a qualidade vocal de pacientes submetidos à laringectomia supracricoide, com voz supraglótica estável, após dois minutos de realização da técnica de firmeza glótica. Foram avaliados seis pacientes, com gravação das amostras de voz em programa computadorizado e nasolaringofibroscopia, antes e após dois minutos de realização da técnica. Para a avaliação perceptivo-auditiva das vozes foi utilizada a escala GRBASI. O tipo de constrição da laringe remanescente, grau de aproximação e amplitude de vibração das estruturas foram avaliados nos exames de nasolaringofibroscopia. Houve aumento da amplitude de vibração das estruturas remanescentes em cinco pacientes, redução da soprosidade em três e da tensão vocal em dois pacientes. Neste estudo inicial com seis indivíduos, observamos que a técnica de firmeza glótica pode ser utilizada como exercício complementar para melhorar a qualidade vocal supraglótica e ampliar a vibração das estruturas remanescentes.Supracricoid horizontal partial laryngectomy is a surgical technique used to treat laryngeal tumors restricted to glottic and supraglottic regions, preserving at

  19. Impact of environmental pollutants on the endocrine regulation of reproduction. Experimental report. Final report; Auswirkungen von Umweltschadstoffen auf die endokrine Regulation der Fortpflanzung. T. A. Sachlicher Bericht. Schlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1992-12-31

    The investigation had the aim to establish the impact of environmentally relevant biocides on different aspects of reproduction. The human granulosa lutein cells used stemmed from patients of an in-vitro fertilization programme. In 29 to 71 per cent of cases, in-vitro exposure of granulosa cells to the biocides PCB, DDT, DDD, DDE, HCB and HCE resulted in reduced progesterone secretions. The impact of biocide exposure on in-vitro secretion of estradiol was less unequivocal. Parallel testing of progesterone and estradiol secretion in the same cell populations showed that biocides do not affect the two steroids in the same degree. The conclusion to be drawn from the results is that steroid, and especially progesterone, production in women who are exposed to lipophilic biocides is impaired. (UG) [Deutsch] Das Ziel der Untersuchung war, inwieweit umweltrelevante Biozide fuer verschiedene Aspekte der Fortpflanzung von Bedeutung sind. Die untersuchten menschlichen Granulosa-Luteinzellen stammten von Patientinnen eines in vitro-Fertilisationsprogrammes. Die in vitro-Exposition von Granulosa-Zellen mit den Bioziden PCB, DDT, DDD, DDE, HCB und HCE fuehrten in 29%-71% zu verringerten Progesteron-Sekretionen. Die Auswirkungen der Biozidbelastung auf die in vitro-Estradiol-Sektretion war weniger eindeutig. Parallele Testung von Progesteron- und Estradiol-Sekretion an den gleichen Zellpopulationen zeigten, dass die Biozidwirkungen auf die beiden Steroide nicht gleichmaessig gerichtet waren. Aufgrund der Ergebnisse muss eine Beeintraechtigung der Steroid-, insbesondere der Progesteron-Produktion, bei der Frau durch Belastung mit lipophilen Bioziden angenommen werden. (UG)

  20. PHILIP GlASS: SCREEN MUSIC

    Directory of Open Access Journals (Sweden)

    Neretina Maina S.

    2012-12-01

    Full Text Available Research is devoted to the music of contemporary composer Philip Glass, which he wrote specifically for the movies including feature and documentary films by American, British and Australian directors (M.Scorsese, E.Morris, S.Daldry, P.Weir etc.. It makes a connection of the composition technologies between his filmmusic and his artmusic written in the aesthetics of minimalism (the repetition and additive processes. The works in collaboration with director G.Reggio highlights as top Glass’ creative at the film industry. It also covers the principles of compilation the composer’s music for a variety of screen opuses, movies and commercials.

  1. Resource Letter GL-1: Gravitational Lensing

    Science.gov (United States)

    Treu, Tommaso; Marshall, Philip J.; Clowe, Douglas

    2012-09-01

    This Resource Letter provides a guide to a selection of the literature on gravitational lensing and its applications. Journal articles, books, popular articles, and websites are cited for the following topics: foundations of gravitational lensing, foundations of cosmology, history of gravitational lensing, strong lensing, weak lensing, and microlensing.

  2. On ambiente de binárias de pequena massa em formação: o caso do glóbulo cometário CG30 e IRAS08076-3556

    Science.gov (United States)

    Hickel, G. R.; Vilas-Boas, J. W. S.

    2003-08-01

    Neste trabalho, combinamos observações de polarização linear no óptico (banda R), dados no infravermelho distante (IRAS) e observações de transições moleculares em radiofreqüências (CO e espécies isotópicas, HCN e HCO+) para analisar o glóbulo cometário (GC) CG30 (na região da IRAS Vela Shell), que apresenta objetos Herbig-Haro e ejeções de matéria, além de uma fonte pontual IRAS em seu interior. Os objetivos deste estudo são: determinar a eficiência de formação estelar nos glóbulos cometários, através da relação entre a massa total do GC e da massa das estrelas em formação; determinar como o campo magnético influencia na formação de estrelas no interior destes objetos; e analisar as modificações que ejeções de matéria de estrelas em formação causam no gás e no campo magnético dos GCs. Combinando nossos dados com trabalhos já publicados, mostramos que CG30 tem uma eficiência de formação estelar em torno de 3%; que o campo magnético é importante na manutenção da estrutura global do GC e demonstra sinais de torção e compressão; e que a ejeção bipolar de matéria das estrelas do par formam uma ejeção quadripolar, a qual influencia na densidade e temperatura do gás e no grau de polarização dos grãos de poeira associados ao gás do GC.

  3. Computational analysis and low-scale constitutive expression of laccases synthetic genes GlLCC1 from Ganoderma lucidum and POXA 1B from Pleurotus ostreatus in Pichia pastoris.

    Science.gov (United States)

    Rivera-Hoyos, Claudia M; Morales-Álvarez, Edwin David; Poveda-Cuevas, Sergio Alejandro; Reyes-Guzmán, Edwin Alfredo; Poutou-Piñales, Raúl A; Reyes-Montaño, Edgar Antonio; Pedroza-Rodríguez, Aura Marina; Rodríguez-Vázquez, Refugio; Cardozo-Bernal, Ángela M

    2015-01-01

    Lacasses are multicopper oxidases that can catalyze aromatic and non-aromatic compounds concomitantly with reduction of molecular oxygen to water. Fungal laccases have generated a growing interest due to their biotechnological potential applications, such as lignocellulosic material delignification, biopulping and biobleaching, wastewater treatment, and transformation of toxic organic pollutants. In this work we selected fungal genes encoding for laccase enzymes GlLCC1 in Ganoderma lucidum and POXA 1B in Pleurotus ostreatus. These genes were optimized for codon use, GC content, and regions generating secondary structures. Laccase proposed computational models, and their interaction with ABTS [2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)] substrate was evaluated by molecular docking. Synthetic genes were cloned under the control of Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase (GAP) constitutive promoter. P. pastoris X-33 was transformed with pGAPZαA-LaccGluc-Stop and pGAPZαA-LaccPost-Stop constructs. Optimization reduced GC content by 47 and 49% for LaccGluc-Stop and LaccPost-Stop genes, respectively. A codon adaptation index of 0.84 was obtained for both genes. 3D structure analysis using SuperPose revealed LaccGluc-Stop is similar to the laccase crystallographic structure 1GYC of Trametes versicolor. Interaction analysis of the 3D models validated through ABTS, demonstrated higher substrate affinity for LaccPost-Stop, in agreement with our experimental results with enzymatic activities of 451.08 ± 6.46 UL-1 compared to activities of 0.13 ± 0.028 UL-1 for LaccGluc-Stop. This study demonstrated that G. lucidum GlLCC1 and P. ostreatus POXA 1B gene optimization resulted in constitutive gene expression under GAP promoter and α-factor leader in P. pastoris. These are important findings in light of recombinant enzyme expression system utility for environmentally friendly designed expression systems, because of the wide range of substrates

  4. Bloqueio do nervo isquiático por abordagem posterior simplificada no ponto médio do sulco glúteo-femoral: estudo com diferentes volumes de lidocaína a 1%

    Directory of Open Access Journals (Sweden)

    Fonseca Neuber Martins

    2006-01-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: O bloqueio do nervo isquiático por via subglútea foi descrito com sucesso em estudo anterior, sendo mais uma opção entre as várias abordagens possíveis. O nervo isquiático torna-se superficial na borda inferior do músculo glúteo máximo, permitindo seu acesso com fácil localização, pouco desconforto e baixo risco de punção acidental de grandes vasos. O objetivo deste estudo foi avaliar o bloqueio do nervo isquiático por esta abordagem simplificada com diferentes volumes de lidocaína a 1%. MÉTODO: Foram estudados 40 pacientes com intervenções cirúrgicas na perna ou no pé distribuídos em dois grupos. Após monitorização, eles foram posicionados em decúbito ventral e realizado bloqueio no ponto médio do sulco glúteo-femoral, com auxílio de neuroestimulador e agulha de 5 cm eletricamente isolada, utilizando 300 mg (G1 ou 200 mg (G2 de lidocaína a 1% sem adrenalina. RESULTADOS: Obteve-se anestesia adequada em todos os casos com o volume e a concentração usados. O tempo de execução do bloqueio foi de 8,6 ? 5,7 min (G1 e 5,6 ? 5,7 min (G2. A latência foi de 5,98 ? 1,4 min (G1 e 6,7 ? 2,9 min (G2. A duração sensitiva e motora do bloqueio foi de 243 ? 37 min e 152 ? 30 min (G1 e 235 ? 39 min e 149 ? 59 min (G2, respectivamente. Não foram observadas diferenças estatísticas significativas entre os grupos estudados. CONCLUSÕES: Essa abordagem é eficaz e de fácil execução, podendo a dose total de anestésico ser reduzida sem comprometimento da qualidade.

  5. Computational Analysis and Low-Scale Constitutive Expression of Laccases Synthetic Genes GlLCC1 from Ganoderma lucidum and POXA 1B from Pleurotus ostreatus in Pichia pastoris

    Science.gov (United States)

    Reyes-Guzmán, Edwin Alfredo; Poutou-Piñales, Raúl A.; Reyes-Montaño, Edgar Antonio; Pedroza-Rodríguez, Aura Marina; Rodríguez-Vázquez, Refugio; Cardozo-Bernal, Ángela M.

    2015-01-01

    Lacasses are multicopper oxidases that can catalyze aromatic and non-aromatic compounds concomitantly with reduction of molecular oxygen to water. Fungal laccases have generated a growing interest due to their biotechnological potential applications, such as lignocellulosic material delignification, biopulping and biobleaching, wastewater treatment, and transformation of toxic organic pollutants. In this work we selected fungal genes encoding for laccase enzymes GlLCC1 in Ganoderma lucidum and POXA 1B in Pleurotus ostreatus. These genes were optimized for codon use, GC content, and regions generating secondary structures. Laccase proposed computational models, and their interaction with ABTS [2, 2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)] substrate was evaluated by molecular docking. Synthetic genes were cloned under the control of Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase (GAP) constitutive promoter. P. pastoris X-33 was transformed with pGAPZαA-LaccGluc-Stop and pGAPZαA-LaccPost-Stop constructs. Optimization reduced GC content by 47 and 49% for LaccGluc-Stop and LaccPost-Stop genes, respectively. A codon adaptation index of 0.84 was obtained for both genes. 3D structure analysis using SuperPose revealed LaccGluc-Stop is similar to the laccase crystallographic structure 1GYC of Trametes versicolor. Interaction analysis of the 3D models validated through ABTS, demonstrated higher substrate affinity for LaccPost-Stop, in agreement with our experimental results with enzymatic activities of 451.08 ± 6.46 UL-1 compared to activities of 0.13 ± 0.028 UL-1 for LaccGluc-Stop. This study demonstrated that G. lucidum GlLCC1 and P. ostreatus POXA 1B gene optimization resulted in constitutive gene expression under GAP promoter and α-factor leader in P. pastoris. These are important findings in light of recombinant enzyme expression system utility for environmentally friendly designed expression systems, because of the wide range of substrates

  6. Computational analysis and low-scale constitutive expression of laccases synthetic genes GlLCC1 from Ganoderma lucidum and POXA 1B from Pleurotus ostreatus in Pichia pastoris.

    Directory of Open Access Journals (Sweden)

    Claudia M Rivera-Hoyos

    Full Text Available Lacasses are multicopper oxidases that can catalyze aromatic and non-aromatic compounds concomitantly with reduction of molecular oxygen to water. Fungal laccases have generated a growing interest due to their biotechnological potential applications, such as lignocellulosic material delignification, biopulping and biobleaching, wastewater treatment, and transformation of toxic organic pollutants. In this work we selected fungal genes encoding for laccase enzymes GlLCC1 in Ganoderma lucidum and POXA 1B in Pleurotus ostreatus. These genes were optimized for codon use, GC content, and regions generating secondary structures. Laccase proposed computational models, and their interaction with ABTS [2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid] substrate was evaluated by molecular docking. Synthetic genes were cloned under the control of Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase (GAP constitutive promoter. P. pastoris X-33 was transformed with pGAPZαA-LaccGluc-Stop and pGAPZαA-LaccPost-Stop constructs. Optimization reduced GC content by 47 and 49% for LaccGluc-Stop and LaccPost-Stop genes, respectively. A codon adaptation index of 0.84 was obtained for both genes. 3D structure analysis using SuperPose revealed LaccGluc-Stop is similar to the laccase crystallographic structure 1GYC of Trametes versicolor. Interaction analysis of the 3D models validated through ABTS, demonstrated higher substrate affinity for LaccPost-Stop, in agreement with our experimental results with enzymatic activities of 451.08 ± 6.46 UL-1 compared to activities of 0.13 ± 0.028 UL-1 for LaccGluc-Stop. This study demonstrated that G. lucidum GlLCC1 and P. ostreatus POXA 1B gene optimization resulted in constitutive gene expression under GAP promoter and α-factor leader in P. pastoris. These are important findings in light of recombinant enzyme expression system utility for environmentally friendly designed expression systems, because of the wide range

  7. Avaliação da concordância da sialometria e cintilografia de glândulas salivares em pacientes com boca seca Evaluation of the concordance of sialometry and salivary glands scintigraphy in dry mouth patients

    Directory of Open Access Journals (Sweden)

    Bianca Maria Liquidato

    2006-02-01

    Full Text Available INTRODUÇÃO: São utilizados vários exames para avaliar os pacientes com queixa de boca seca e, especialmente, os pacientes com Síndrome de Sjögren, em que estes exames fazem parte de critérios de classificação para estudos científicos. OBJETIVO: Desta maneira, procurou-se avaliar se haveria concordância entre os resultados da sialometria e da cintilografia de glândulas salivares para, se esta concordância estiver presente, optar por apenas um dos dois exames. CASUÍSTICA E MÉTODO: Foram avaliados 72 pacientes com boca seca, divididos em grupos não-Síndrome de Sjögren, com Síndrome de Sjögren primária e com Síndrome de Sjögren secundária. Os resultados de sialometria e cintilografia de glândulas salivares foram estudados, procurando-se dimensionar a concordância existente entre eles, através do teste de Kappa. RESULTADOS: Observou-se concordância igual ou próxima de zero entre os dois testes. CONCLUSÃO: Pelos resultados observados, não é possível fazer a opção por um ou outro exame, devendo ambos serem realizados.INTRODUCTION: Many diagnostic tests are used to evaluate dry mouth patients, especially the ones with Sjögren’s Syndrome, to whom these tests are part of classification criteria for scientific studies. AIM: Thus, the concordance between results of sialometry and salivary glands scintigraphy was evaluated; if positive, it would enable the choice of one or the other for diagnosis. PATIENTS AND METHOD: Seventy-two dry mouth patients were divided into non-Sjögren’s Syndrome group, primary Sjögren’s Syndrome group and secondary Sjögren’s Syndrome group. The concordance among sialometry and scintigraphy results was evaluated by Kappa test. RESULTS: It was observed that their concordance was equal or near to zero. CONCLUSION: It is not possible to make a choice between these tests and both should be performed.

  8. Development and characterization of edible films based on gluten from semi-hard and soft Brazilian wheat flours (development of films based on gluten from wheat flours Desenvolvimento e caracterização de filmes comestíveis de glúten de farinhas fortes e fracas de trigos brasileiros

    Directory of Open Access Journals (Sweden)

    Patrícia Sayuri Tanada-Palmu

    2003-08-01

    Full Text Available Edible films based on gluten from four types of Brazilian wheat gluten (2 "semi-hard" and 2 "soft" were prepared and mechanical and barrier properties were compared with those of wheat gluten films with vital gluten. Water vapor, oxygen permeability, tensile strength and percent elongation at break, solubility in water and surface morphology were measured. The films from "semi-hard" wheat flours showed similar water vapor permeability and solubility in water to films from vital gluten and better tensile strength than the films from "soft" and vital gluten. The films from vital gluten had higher elongation at break and oxygen permeability and also lower solubility in water than the films from the Brazilian wheat "soft" flours. In spite of the vital gluten showed greater mechanical resistance, desirable for the bakery products, for the purpose of developing gluten films Brazilian "semi-hard" wheat flours can be used instead of vital gluten, since they showed similar barrier and mechanical properties.Filmes à base de glúten de quatro tipos de farinhas de trigo brasileiras (2 "semi-fortes " e 2 "fracas" foram preparados e suas propriedades mecânicas e de barreira foram comparadas com filmes com glúten vital (comercial. Permeabilidade ao vapor d'água e oxigênio, resistência à tração, porcentagem de elongação na ruptura, solubilidade em água e morfologia de superfície foram medidas. Filmes de glúten das farinhas "semi-fortes" mostraram similar permeabilidade ao vapor d'água e solubilidade em água em comparação aos filmes de glúten vital e melhor resistência à tração do que os filmes das farinhas "fracas" e glúten vital. O filme de glúten vital apresentou maior elongação na ruptura e permeabilidade ao oxigênio do que os filmes das farinhas brasileiras e ainda mais baixa solubilidade que as farinhas fracas. Apesar do glúten vital ter uma grande resistência mecânica, desejável para produtos de panificação, para o prop

  9. Comparison of the protein and fatty acid fraction of Balkan donkey and human milk

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    Jasmina Gubić

    2015-07-01

    Full Text Available The aim of this study was to compare the protein and fatty acid fractions of Balkan donkey and human milk in the early lactation stage (40 and 90 day. This study revealed that donkey milk contains αs1-casein (1.38-1.89 g/L and higher concentration of β-casein (0.1-0.55 g/L in comparison to human milk. The concentration of α-lactalbumin increased during the lactation phases from 40 to 90 days in both types of milk. Donkey milk contained β-lactoglobulin in low concentrations which decreased to 90th day of lactation. Donkey milk was particularly rich in two whey proteins, lactoferrin and lysozyme, which were found to have molecular weight of approximately 76 kDa and 14.9-15.4 kDa, respectively. The content of lysozyme in donkey milk ranged from 2.39 to 2.97 g/L, while human milk contained 30-50 times lower concentrations of lysozyme in comparison to donkey milk. Thus, donkey milk contained also a higher concentration of lactoferrin (0.012-0.25 g/L than it was found in the human milk. Lysozyme and lactoferrin content in donkey milk increased during the period from 40th to 90th day of lactation. The percentage of total SFA, MUFA and PUFA was similar in donkey and human milk. The content of essential fatty acids increased during 40-90 days of lactation and was approximately 2.5 times higher in comparison to human milk.

  10. Human Milk Oligosaccharides and Synthetic Galactosyloligosaccharides Contain 3′-, 4-, and 6′-Galactosyllactose and Attenuate Inflammation in Human T84, NCM-460, and H4 Cells and Intestinal Tissue Ex Vivo12

    Science.gov (United States)

    Ko, Jae Sung; Leone, Serena; Nanthakumar, N Nanda

    2016-01-01

    Background: The immature intestinal mucosa responds excessively to inflammatory insult, but human milk protects infants from intestinal inflammation. The ability of galactosyllactoses [galactosyloligosaccharides (GOS)], newly found in human milk oligosaccharides (HMOS), to suppress inflammation was not known. Objective: The objective was to test whether GOS can directly attenuate inflammation and to explore the components of immune signaling modulated by GOS. Methods: Galactosyllactose composition was measured in sequential human milk samples from days 1 through 21 of lactation and in random colostrum samples from 38 mothers. Immature [human normal fetal intestinal epithelial cell (H4)] and mature [human metastatic colonic epithelial cell (T84) and human normal colon mucosal epithelial cell (NCM-460)] enterocyte cell lines were treated with the pro-inflammatory molecules tumor necrosis factor-α (TNF-α) or interleukin-1β (IL-1β) or infected with Salmonella or Listeria. The inflammatory response was measured as induction of IL-8, monocyte chemoattractant protein 1 (MCP-1), or macrophage inflammatory protein-3α (MIP-3α) protein by ELISA and mRNA by quantitative reverse transcriptase-polymerase chain reaction. The ability of HMOS or synthetic GOS to attenuate this inflammation was tested in vitro and in immature human intestinal tissue ex vivo. Results: The 3 galactosyllactoses (3′-GL, 4-GL, and 6′-GL) expressed in colostrum rapidly declined over early lactation (P milk, contributing to innate immune modulation. The potential clinical utility of galactosyllactose warrants investigation. PMID:26701795

  11. Human Milk Oligosaccharides and Synthetic Galactosyloligosaccharides Contain 3'-, 4-, and 6'-Galactosyllactose and Attenuate Inflammation in Human T84, NCM-460, and H4 Cells and Intestinal Tissue Ex Vivo.

    Science.gov (United States)

    Newburg, David S; Ko, Jae Sung; Leone, Serena; Nanthakumar, N Nanda

    2016-02-01

    The immature intestinal mucosa responds excessively to inflammatory insult, but human milk protects infants from intestinal inflammation. The ability of galactosyllactoses [galactosyloligosaccharides (GOS)], newly found in human milk oligosaccharides (HMOS), to suppress inflammation was not known. The objective was to test whether GOS can directly attenuate inflammation and to explore the components of immune signaling modulated by GOS. Galactosyllactose composition was measured in sequential human milk samples from days 1 through 21 of lactation and in random colostrum samples from 38 mothers. Immature [human normal fetal intestinal epithelial cell (H4)] and mature [human metastatic colonic epithelial cell (T84) and human normal colon mucosal epithelial cell (NCM-460)] enterocyte cell lines were treated with the pro-inflammatory molecules tumor necrosis factor-α (TNF-α) or interleukin-1β (IL-1β) or infected with Salmonella or Listeria. The inflammatory response was measured as induction of IL-8, monocyte chemoattractant protein 1 (MCP-1), or macrophage inflammatory protein-3α (MIP-3α) protein by ELISA and mRNA by quantitative reverse transcriptase-polymerase chain reaction. The ability of HMOS or synthetic GOS to attenuate this inflammation was tested in vitro and in immature human intestinal tissue ex vivo. The 3 galactosyllactoses (3'-GL, 4-GL, and 6'-GL) expressed in colostrum rapidly declined over early lactation (P milk, contributing to innate immune modulation. The potential clinical utility of galactosyllactose warrants investigation. © 2016 American Society for Nutrition.

  12. Impact of light spectral composition on the length and weight of the gametophyte of Polytrichastrum formosum (Hedw. G.L. Smith, Plagiomnium cuspidatum (Hedw. T.J. Kop. and Pleurozium schreberi (Brid. Mitt.

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    Katarzyna Możdżeń

    2014-04-01

    Full Text Available The aim of the present study was to examine the influence of light spectral composition on the length and weight of mosses gametophytes: Polytrichastrum formosum (Hedw. G.L. Smith, Plagiomnium cuspidatum (Hedw. T.J. Kop. and Pleurozium schreberi (Brid. Mitt. Plants were grown 31 days in the chambers equipped with LEDs matrices of the same intensity of light (200 μmol m-2 · s-1, but different spectral composition: white (WL, white-blue (WBL and red-green-blue (RGBL. It was found that the WBL as compared to RGBL inhibited the growth of the whole gametophytes of P. cuspidatum and P. formosum. WBL inhibited also rhizoids length of P. cupsidatum, in comparison to plants growing on WL and RGBL as well as growth of leaves stalks P. schreberi and P. cuspidatum as compared to WL. For RGBL fresh weight of plants P. cuspidatum was significantly higher than the WBL, while in P. schreberi higher than that for WBL and WL. Impact of light quality on the dry matter production was observed only in P. schreberi. Effect of spectral composition of light on the length and weight of the gametophyte depends on the species of moss.

  13. A comparative examination of the adsorption mechanism of an anionic textile dye (RBY 3GL) onto the powdered activated carbon (PAC) using various the isotherm models and kinetics equations with linear and non-linear methods

    Science.gov (United States)

    Açıkyıldız, Metin; Gürses, Ahmet; Güneş, Kübra; Yalvaç, Duygu

    2015-11-01

    The present study was designed to compare the linear and non-linear methods used to check the compliance of the experimental data corresponding to the isotherm models (Langmuir, Freundlich, and Redlich-Peterson) and kinetics equations (pseudo-first order and pseudo-second order). In this context, adsorption experiments were carried out to remove an anionic dye, Remazol Brillant Yellow 3GL (RBY), from its aqueous solutions using a commercial activated carbon as a sorbent. The effects of contact time, initial RBY concentration, and temperature onto adsorbed amount were investigated. The amount of dye adsorbed increased with increased adsorption time and the adsorption equilibrium was attained after 240 min. The amount of dye adsorbed enhanced with increased temperature, suggesting that the adsorption process is endothermic. The experimental data was analyzed using the Langmuir, Freundlich, and Redlich-Peterson isotherm equations in order to predict adsorption isotherm. It was determined that the isotherm data were fitted to the Langmuir and Redlich-Peterson isotherms. The adsorption process was also found to follow a pseudo second-order kinetic model. According to the kinetic and isotherm data, it was found that the determination coefficients obtained from linear method were higher than those obtained from non-linear method.

  14. Cytotoxicity on Human Cancer Cells of Ophidiacerebrosides Isolated from the African Starfish Narcissia canariensis

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    Fereshteh Farokhi

    2010-12-01

    Full Text Available The starfish Narcissia canariensis harvested from the coasts off Dakar, Senegal, was investigated for glycolipids (GL. This report deals with the isolation, characterization and biological activity of a fraction F13-3 separated from the GL mixture and selected according to its ability to inhibit KB cell proliferation after 72 hours of treatment. Firstly, a GL mixture F13 was obtained that accounted for 1.36% of starfish biomass (dry weight and 0.36% of total lipids. The fraction F13-3 obtained from F13 contained three homologous GL identified as peracetylated derivatives on the basis of chemical and spectroscopic evidence. These contained a β-glucopyranoside as sugar head, a 9-methyl-branched 4,8,10-triunsaturated long-chain aminoalcohol as sphingoid base and amide-linked 2-hydroxy fatty acid chains. The majority (63% had an amide-linked 2‑hydroxydocosanoic acid chain and was identified as the ophidiacerebroside-C, firstly isolated from the starfish Ophidiaster ophidiamus. The minor components of F13-3 differed by one more or one less methylene group, and corresponded to ophidiacerebroside-B and -D. We found that F13-3 displayed an interesting cytotoxic activity over 24 hours on various adherent human cancerous cell lines (multiple myeloma, colorectal adenocarcinoma and glioblastoma multiforme with an IC50 of around 20 μM.

  15. Cytotoxicity on human cancer cells of ophidiacerebrosides isolated from the African starfish Narcissia canariensis.

    Science.gov (United States)

    Farokhi, Fereshteh; Wielgosz-Collin, Gaetane; Clement, Monique; Kornprobst, Jean-Michel; Barnathan, Gilles

    2010-12-22

    The starfish Narcissia canariensis harvested from the coasts off Dakar, Senegal, was investigated for glycolipids (GL). This report deals with the isolation, characterization and biological activity of a fraction F13-3 separated from the GL mixture and selected according to its ability to inhibit KB cell proliferation after 72 hours of treatment. Firstly, a GL mixture F13 was obtained that accounted for 1.36% of starfish biomass (dry weight) and 0.36% of total lipids. The fraction F13-3 obtained from F13 contained three homologous GL identified as peracetylated derivatives on the basis of chemical and spectroscopic evidence. These contained a β-glucopyranoside as sugar head, a 9-methyl-branched 4,8,10-triunsaturated long-chain aminoalcohol as sphingoid base and amide-linked 2-hydroxy fatty acid chains. The majority (63%) had an amide-linked 2-hydroxydocosanoic acid chain and was identified as the ophidiacerebroside-C, firstly isolated from the starfish Ophidiaster ophidiamus. The minor components of F13-3 differed by one more or one less methylene group, and corresponded to ophidiacerebroside-B and -D. We found that F13-3 displayed an interesting cytotoxic activity over 24 hours on various adherent human cancerous cell lines (multiple myeloma, colorectal adenocarcinoma and glioblastoma multiforme) with an IC(50) of around 20 μM.

  16. Hemorragia digestiva alta secundária a hamartoma de glândulas de Brunner: relato de caso e revisão da literatura = Upper gastrintestinal bleeding due to Brunner’s gland hamartoma: case report and literature review

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    Lul, Rodrigo Mayer

    2013-01-01

    Conclusões: Apesar do hamartoma de glândulas de Brunner ser uma lesão benigna e rara, de crescimento indolente e comumente assintomática, pode provocar quadros graves como o descrito neste relato, devendo ser sempre lembrado como diagnóstico diferencial em casos de hemorragia digestiva alta

  17. Avaliação ultrassonográfica e mensurações das glândulas adrenais em primatas não humanos neotropicais: mico-de-cheiro (Saimiri sciureus, macaco-da-noite (Aotus azarae infulatus e bugio-ruivo (Alouatta guariba clamitans

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    Andressa C. Souza

    2014-09-01

    Full Text Available As glândulas adrenais possuem funções endócrinas relacionadas a múltiplas funções vitais, estando intimamente relacionadas à capacidade do animal em se adaptar ao estresse. O exame ultrassonográfico é o método diagnóstico de escolha para avaliação das glândulas em diferentes espécies. Considerando a escassa literatura, questiona-se se as doenças adrenais em primatas não humanos são incomuns ou subdiagnosticadas, havendo a hipótese desse fato ser determinado pela falta de parâmetros. Objetivou-se descrever as características ultrassonográficas das glândulas adrenais para três espécies de primatas não humanos mantidas em cativeiro: Saimiri sciureus (mico-de-cheiro, Aotus azarae infulatus (macaco-da-noite e Alouatta guariba clamitans (bugio-ruivo. Conclui-se que é possível a identificação das glândulas adrenais por meio de exame ultrassonográfico, sendo que os padrões de referência foram estabelecidos com sucesso para as espécies em questão. Ressalta-se que a adequação de animais em ambientes estressantes é frequentemente acompanhada por uma hipertrofia das glândulas adrenais, portanto deve-se levar em consideração que as mensurações realizadas nesse estudo foram estabelecidas em animais de cativeiro.

  18. Frecuencia de las mutaciones en los genes BRCA en mujeres con agregación familiar de cáncer de glándula mamaria/ovario

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    Jennyfer Dahianna Benavides-Cerquera

    2016-07-01

    Full Text Available Objetivo: Reunir evidencias de la variación en la frecuencia de las mutaciones de BRCA1 y BRCA2 y la historia familiar en pacientes con cáncer de glándula mamaria (CGM y cáncer de ovario (CO de diferentes orígenes geográficos. Método: En este trabajo se realizó una revisión sistemática, siguiendo los parámetros del protocolo PRISMA, para estimar la prevalencia de mutaciones en los genes BRCA 1/2 en pacientes con CGM y CO, la incidencia de la historia familiar y la prevalencia observada en casos esporádicos en este tipo de cáncer. Resultados: Se observa una heterogeneidad en la frecuencia de las mutaciones de estos genes en los estudios de historia familiar, con una variación entre 0.0 y 0.48 en pacientes y familiares con CGM y CO similares a los previamente reportados. Discusión: Este amplio rango de la frecuencia se debe al origen de la población estudiada, el número de individuos analizados y la metodología de genotipificación utilizada. La revisión revela que el CGM y CO familiar es dos veces más frecuente, en comparación con los casos de esta misma patología con origen esporádico. Conclusiones: Este tipo de estudios moleculares les permite a las personas que presentan historia familiar con CGM y CO realizarse análisis precoces y chequeos para prevenir en un futuro el desarrollo de alguna de estas neoplasias.

  19. Frecuencia de las mutaciones en los genes BRCA en mujeres con agregación familiar de cáncer de glándula mamaria/ovario

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    Jennyfer Dahianna Benavides-Cerquera

    2016-01-01

    Full Text Available Objetivo: Reunir evidencias de la variación en la frecuencia de las mutaciones de BRCA1 y BRCA2 y la historia familiar en pacientes con cáncer de glándula mamaria (CGM y cáncer de ovario (CO de diferentes orígenes geográficos. Método: En este trabajo se realizó una revisión sistemática, siguiendo los parámetros del protocolo PRISMA, para estimar la prevalencia de mutaciones en los genes BRCA 1/2 en pacientes con CGM y CO, la incidencia de la historia familiar y la prevalencia observada en casos esporádicos en este tipo de cáncer. Resultados: Se observa una heterogeneidad en la frecuencia de las mutaciones de estos genes en los estudios de historia familiar, con una variación entre 0.0 y 0.48 en pacientes y familiares con CGM y CO similares a los previamente reportados. Discusión: Este amplio rango de la frecuencia se debe al origen de la población estudiada, el número de individuos analizados y la metodología de genotipificación utilizada. La revisión revela que el CGM y CO familiar es dos veces más frecuente, en comparación con los casos de esta misma patología con origen esporádico. Conclusiones: Este tipo de estudios moleculares les permite a las personas que presentan historia familiar con CGM y CO realizarse análisis precoces y chequeos para prevenir en un futuro el desarrollo de alguna de estas neoplasias.

  20. Estudo clinicopatológico de 106 adenomas pleomórficos de glândula salivar maior A clinicopathologic study of 106 pleomorphic adenomas of the major salivary glands

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    Jerlucia Cavalcanti das Neves

    2007-10-01

    Full Text Available INTRODUÇÃO: O adenoma pleomórfico (AP é o mais comum dos tumores das glândulas salivares. Transformação maligna pode ocorrer após recorrências ou em casos com longo tempo de evolução. OBJETIVO: Analisar os aspectos clinicoepidemiológicos e anatomopatológicos do AP de glândula salivar maior, principalmente os considerados indício de transformação maligna. MATERIAL E MÉTODO: Foram avaliados 106 casos de AP pela pesquisa retrospectiva nos prontuários clínicos e revisão das preparações histológicas. RESULTADOS: A média de idade dos pacientes foi de 39,51 anos, houve predomínio do sexo feminino (69,5%, a glândula parótida foi a mais acometida (86,8% e o tempo de evolução foi superior a um ano em 76,74% dos casos, com tamanho tumoral de 3,48 cm em média. A avaliação histopatológica demonstrou cápsula tumoral incompleta e delgada em 49% dos casos. Protrusões para a cápsula ou extensão extracapsular foram infreqüentes, 11,32% e 8,49%, respectivamente. A matriz extracelular variou entre mixocondróide (41,5%, mixóide (36,8%, condróide (3,8% e fibrótica (1,9%, tendo sido observadas associações entre os diversos tipos. O componente epitelial/mioepitelial constituiu 50% ou mais do tumor em 65,1% dos casos e estava disposto em arranjos cordonal (86,8%, ductal (81,1%, sólido (40,6%, cístico (20,7% e em "paliçada" (8,49%. Metaplasia escamosa ocorreu em 16,04% dos casos. Alterações histopatológicas relacionadas com transformação maligna foram incomuns: hialinização extensa (4,7% e necrose (0,9%. CONCLUSÕES: confirma-se a diversidade histopatológica do AP. Características associadas a transformação maligna não foram freqüentes na amostra.INTRODUCTION: Pleomorphic adenomas (PA are the most common histologic type of salivary gland tumors. Malignant transformation may occur in the setting of recurrent or longstanding disease. OBJECTIVE: Evaluate the clinicopathologic characteristics of PA of the major

  1. Substituição do farelo de soja pela farinha de glúten de milho na alimentação de cabras leiteiras Substitution of soybean meal protein by corn gluten meal protein in dairy goat feeding

    Directory of Open Access Journals (Sweden)

    Luiz Gonzaga Pego de Macedo

    2003-08-01

    Full Text Available Realizou-se um experimento com cabras Saanen, primíparas, pesando em média, 50 kg, alojadas em baias individuais de 3 m², objetivando avaliar a substituição da proteína do farelo de soja (FS pela proteína da farinha de glúten de milho (FGM, na produção e composição do leite, consumo voluntário e níveis de uréia plasmática. O delineamento experimental foi o triplo quadrado latino 4x4, com quatro períodos de 21 dias, sendo 14 dias de adaptação à dieta e sete dias para colheita das amostras. As cabras foram alimentadas e ordenhadas pela manhã e tarde. Os níveis de substituição estudados foram 0, 10, 30 e 50% de FGM (base na proteína bruta. A substituição do farelo de soja pela farinha de glúten de milho não afetou o consumo (kg/dia e %PV de matéria seca, proteína bruta e fibra em detergente ácido, mas houve efeito quadrático, para o consumo de fibra em detergente neutro (kg/dia e % PV. Houve efeito sobre os níveis de uréia plasmática (UP, que foram inferiores para os menores níveis de substituição, sendo os maiores valores de UP observados para o tratamento que possuía somente FS. A produção de leite decresceu linearmente com a inclusão da farinha de glúten de milho. Os níveis de substituição resultaram em decréscimos lineares na produção de gordura (kg/dia, no teor de gordura do leite (% e no teor de sólidos totais. Houve efeito quadrático para produção de lactose (kg/dia, observando-se o menor valor estimado para o nível de substituição de 31,6%. Não foi observado efeito sobre a produção de proteína bruta no leite, cujo valor médio foi de 0,083 kg/dia. Os teores de proteina bruta, lactose e sólidos totais não sofreram efeito dos níveis de substituição, sendo os valores médios percentuais de 2,98; 4,35 e 11,51%, respectivamente.It was carried out an experiment with goats Saanen, on the first lactation, with 50 kg of body weight, housed in individual stalls of 3 m², to evaluate

  2. Potent anti-cancer effects of citrus peel flavonoids in human prostate xenograft tumors.

    Science.gov (United States)

    Lai, Ching-Shu; Li, Shiming; Miyauchi, Yutaka; Suzawa, Michiko; Ho, Chi-Tang; Pan, Min-Hsiung

    2013-06-01

    Prostate cancer is one of the most prevalent malignancies and is the second leading cause of cancer-related deaths in men. Fruit and vegetable consumption is a novel, non-toxic therapeutic approach that can be used to prevent and treat prostate cancer. Citrus peels and their extracts have been reported to have potent pharmacological activities and health benefits due to the abundance of flavonoids in citrus fruits, particularly in the peels. Our previous studies demonstrated that oral administration of Gold Lotion (GL), an extract of multiple varieties of citrus peels containing abundant flavonoids, including a large percentage of polymethoxyflavones (PMFs), effectively suppressed azoxymethane (AOM)-induced colonic tumorigenesis. However, the efficacy of GL against prostate cancer has not yet been investigated. Here, we explored the anti-tumor effects of GL using a human prostate tumor xenograft mouse model. Our data demonstrated that treatment with GL by both intraperitoneal (i.p.) injection and oral administration dramatically reduced both the weights (57%-100% inhibition) and volumes (78%-94% inhibition) of the tumors without any observed toxicity. These inhibitory effects were accompanied by mechanistic down-regulation of the protein levels of inflammatory enzymes (inducible nitric oxide synthase, iNOS and cyclooxygenase-2, COX-2), metastasis (matrix metallopeptidase-2, MMP-2 and MMP-9), angiogenesis (vascular endothelial growth factor, VEGF), and proliferative molecules, as well as by the induction of apoptosis in prostate tumors. Our findings suggest that GL is an effective anti-cancer agent that may potentially serve as a novel therapeutic option for prostate cancer treatment.

  3. Effect of culture media on expansion properties of human umbilical cord matrix-derived mesenchymal cells.

    Science.gov (United States)

    Salehinejad, Parvin; Alitheen, Noorjahan Banu; Nematollahi-Mahani, Seyed Noureddin; Ali, Abdul Manaf; Omar, Abdul Rahman; Janzamin, Ehsan; Hajghani, Masoomeh

    2012-09-01

    Mesenchymal stromal cells (MSC) have been isolated from a number of different tissues, including umbilical cord. Because of the lack of a uniform approach to human umbilical cord matrix-derived mesenchymal (hUCM) cell expansion, we attempted to identify the optimum conditions for the production of a high quantity of hUCM cells by comparing two media. We compared the ability of Dulbecco's Modified Eagle's Medium/F12 (DMEM/F12) and Alpha Minimum Essential Medium (α-MEM) with Glutamax (GL) (α-MEM/GL) to expand hUCM cells. For this purpose, hUCM cells were cultured in plates containing different culture media supplemented with 10% fetal bovine serum (FBS). Culture dishes were left undisturbed for 10-14 days to allow propagation of the newly formed hUCM cells. The expansion properties, CD marker expression, differentiation potential, population doubling time (PDT) and cell activity were compared between the two groups. The hUCM cells harvested from each group were positive for MSC markers, including CD44, CD90 and CD105, while they were negative for the hematopoietic cell surface marker CD34. Differentiation into adipogenic and osteogenic lineages was confirmed for both treatments. Cell activity was higher in the α-MEM/GL group than the DMEM/F12 group. PDT was calculated to be 60 h for the DMEM/F12 group, while for the α-MEM/GL group it was 47 h. Our data reveal that α-MEM/GL with 10% FBS supports hUCM cell growth more strongly than DMEM/F12 with 10% FBS.

  4. Autotaxin inhibition with PF8380 enhances the radiosensitivity of human and murine glioblastoma cell lines

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    Sandeep R Bhave

    2013-09-01

    Full Text Available Purpose: Glioblastoma multiforme (GBM is an aggressive primary brain tumor that is radio-resistant and recurs despite aggressive surgery, chemo and radiotherapy. Autotaxin (ATX is over expressed in various cancers including GBM and is implicated in tumor progression, invasion, and angiogenesis. Using the ATX specific inhibitor, PF-8380, we studied ATX as a potential target to enhance radiosensitivity in GBM.Methods and Materials: Mouse GL-261 and Human U87MG cells were used as GBM cell models. Clonogenic survival assays and tumor transwell invasion assays were performed using PF-8380 to evaluate role of ATX in survival and invasion. Radiation dependent activation of Akt was analyzed by immunoblotting. Tumor induced angiogenesis was studied using the dorsal skin-fold model in Gl-261. Heterotopic mouse GL-261 tumors were used to evaluate the efficacy of PF-8380 as a radiosensitizer.Results: Pretreatment of GL-261 and U87-MG cells with 1µM PF-8380 followed by 4Gy irradiation resulted in decreased clonogenic survival, decreased migration (33% in GL-261;P = 0.002 and 17.9% in U87; P = 0.012 decreased invasion (35.6% in GL-261; P = 0.0037 and 31.8% in U87; P = 0.002, and attenuated radiation induced Akt phosphorylation. In the tumor window model inhibition of ATX abrogated radiation-induced tumor neovascularization (65%; P=0.011. In a heterotopic mouse GL-261 tumors untreated mice took 11.2 days to reach a tumor volume of 7000 mm3 , however combination of PF-8380 (10mg/kg with irradiation (5 fractions of 2Gy took more than 32 days to reach a tumor volume of 7000 mm3 .Conclusion: Inhibition of ATX by PF8380 led to decreased invasion and enhanced radiosensitization of glioma cells. Radiation induced activation of Akt was abrogated by inhibition of ATX. Furthermore, inhibition of ATX led to diminished tumor vascularity and delayed tumor growth. These results suggest that inhibition of ATX may ameliorate glioblastoma response to radiotherapy.

  5. Afican Health Sciences Vol 10 No 4.pmd

    African Journals Online (AJOL)

    Administrator

    SI, secondary interstitial cells; AF, antral follicle; PAF, preantral follicle; PF, primordial follicle; ZP, zona pellucida; O, oocyte. All pictures are photographed at a magnification of X100. The granulosa lutein cells of most of the corpora lutea in the ovaries of extract treated animas showed abnormal features such as appearance ...

  6. Specialised structural descriptions for human body parts: Evidence from autotopagnosia.

    Science.gov (United States)

    Buxbaum, L J; Coslett, H B

    2001-06-01

    Previous accounts of autotopagnosia (e.g., Ogden, 1985; Pick, 1908; Semenza, 1988) propose that the disorder is attributable to deficits in "mental images," visual body schema, or semantic representations. A recent account (Sirigu, Grafman, Bressler, & Sunderland, 1991b) posits deficits in visual structural descriptions of the human body and its parts, in the context of spared semantic and proprioceptivespatio-motor body representations, but provides no evidence bearing on the nature or format of the putatively damaged representation. We report data from a man with autotopagnosia consequent to lefthemisphere brain damage which bear directly on the nature of the representation impaired in the disorder. The subject, GL, is unable to localise body parts on himself or others, whether cued by verbal or visual input. In contrast, he uses body parts precisely in reaching and grasping tasks, correctly matches items of clothing to body parts, and localises the parts of animals and man-made objects without error. We also demonstrate that GL is unable to match pictured or real human body parts across shifts in orientation or changes in visual appearance, but can perform analogous matching tasks with animal body parts and man-made object parts. The data extend the account of Sirigu et al. (1991b) in suggesting that human body part localisation depends upon structural descriptions of human (but not animal) bodies that enable viewpoint-independent body part recognition and participate in the calculation of equivalence between the body parts of self and others across transformations in orientation.

  7. Criterios de médicos anestesiólogos de ciudad de La Habana sobre el hematócrito perioperatorio y la indicación de glóbulos

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    Rosa María Abad Hernández

    2001-03-01

    Full Text Available Se informa que la terapéutica transfusional sufrió importantes cambios en la última década. Uno de ellos lo constituye el valor de hemoglobina aceptado durante el perioperatorio. Con el objetivo de conocer los criterios de una muestra de médicos-anestesiólogos de Ciudad de La Habana se realizó una encuesta en diferentes hospitales y se llegó a los siguientes resultados: 1. El 68 % de los médicos exige una hemoglobina de 10 g % para pacientes operados electivamente. 2. El 25 % de los médicos no exige una hemoglobina de 10 g % para operaciones urgentes. 3. El 2 % de los médicos transfunde intraoperatoriamente, porque la hemoglobina preoperatoria sea inferior a 10 g % y el 10 % por sangramiento mayor de 500 mL. 4. Para transfundir intraoperatoriamente glóbulos rojos a los pacientes toman en consideración los siguientes aspectos: a compromiso del transporte de oxígeno; b inestabilidad hemodinámica; c hematócritos mínimos, siempre inferiores al 30 % y d diferentes tanto por cientos de volumen perdidoThe significant changes occurred in transfusional therapeutics in the last decade are reported in this paper. One of them is the haemoglobin value accepted during the perioperative period. A survey was done in different hospitals of Havana City in order to know the criteria of a sample of anesthesiologists. The results were as follows: l. 68% of the anesthesiologists demand a haemoglobin of 10g % for patients undergoing elective surgery. 2. 25% of the patients do not require a haemoglobin of 10 g % for urgent operations. 3. 2% of the anesthesiologists transfuse intraoperatively when preoperative haemoglobin is under 10 g % and 10% do it due to bleeding over 500 mL. 4. To transfuse red blood cells intraoperatively to patients anesthesiologists take into consideration the following aspects: a compromise of oxygen transport, b hemodyanmic inestability; c minimum haematocrits, always under 30% and d different percentages of lost volume

  8. Massa alimentícia sem glúten com elevado teor proteico obtida por processo convencional Gluten-free pasta with high protein content obtained by conventional processing

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    Marcio Schmiele

    2013-05-01

    Full Text Available Indivíduos celíacos necessitam de dieta sem glúten como, por exemplo, massas alimentícias de arroz. No entanto, esse cereal apresenta o menor teor de proteínas, em relação aos demais cereais, e deficiência em características tecnológicas de cozimento e textura para as massas alimentícias. O objetivo deste trabalho foi desenvolver massa de farinha de arroz com alto teor de proteína através de Delineamento Composto Central Rotacional de três variáveis independentes, sendo: X1 - isolado proteico de soja (IPS - 0-20%; X2 - albumina de ovo modificada e desidratada (ALB - 0-15%; e X3 - farinha de arroz pré-gelatinizada (FAG - 0-30%. As massas foram analisadas quanto a tempo de cozimento (TC, perda de sólidos na água de cozimento (PS, aumento de peso (AP, firmeza e adesividade. Verificou-se que a adição de IPS, ALB e FAG influenciou significativamente (PCeliac individuals require a gluten free diet, which includes products such as rice pasta. However, this cereal has lower protein content, when compared with other cereals, and poor cooking and texture technological characteristics for pasta. The aim of this research was the development of rice pasta with high protein content through a Central and Rotational Composite Design with three independent variables, as follows: X1 - isolated soy protein (ISP - 0-20 %; X2 - modified and dehydrated egg albumin (ALB - 0-15 %; and X3 - pre-gelatinized rice flour (GRF - 0-30%. The pasta samples were analyzed for cooking time (CT, solid loss in cooking water (SL, weight gain (WG, firmness and adhesiveness. It was verified that ISP, ALB and GRF significantly influenced (P<0.10 CT, SL and firmness, with values between 300 and 525s, 2.22 and 9.84% and 4.82 and 11.13N, respectively. The optimum point was obtained with the addition of 17% ISP, 15% ALB and 15% GRF, indicating a product with excellent cooking and texture properties to include in the diet of celiacs.

  9. Estudo dos fatores impregnação e secagem nas características de glóbulos utilizados em homeopatia Study of impregnation and drying factors in the characteristics of globules used in homeopathy

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    Eliana E. Diehl

    2008-03-01

    Full Text Available Glóbulos são uma das formas farmacêuticas mais dispensadas em farmácias homeopáticas no Brasil. Este trabalho teve como objetivo comparar técnicas de impregnação especificadas na Farmacopéia Homeopática Brasileira 2ª Edição e Manual de Normas Técnicas para Farmácia Homeopática 3ª Edição e na prática em farmácias homeopáticas do município de Florianópolis, Santa Catarina, Brasil. As variáveis de formulação percentual de insumo ativo (3 e 10% e tipo de impregnação (simples e tríplice e a variável de processo temperatura de secagem (20 e 50ºC foram analisadas através de planejamento fatorial 2³. As respostas estudadas foram o peso antes e após impregnação e o tempo de desagregação. Os resultados mostraram maior diferença de peso com impregnação a 10 % e secagem a 50ºC, independente do tipo de impregnação. O tempo de desagregação não foi influenciado pelas variáveis em estudo (p Globules are one of the most dosage forms dispensed in homeopathic pharmacies in Brazil. This work aimed at comparing different impregnation techniques specified in the Brazilian Homeopathic Pharmacopoeia 2 Edition and Manual of Technical Norms for Homeopathic Pharmacy 3 Edition and the practical in homeopathic pharmacies in the city of Florianópolis, state of Santa Catarina, Brazil. The formulations variables percent active raw material (3 and 10%, impregnation type (simple and triple and the process variable drying temperature (20 and 50ºC were analyzed through a factorial design 2³. The studied answers were the weight before and after the impregnation and time of disaggregation. The results show a larger weight difference with the 10% impregnation and the drying of 50ºC, regardless of the impregnation type. The time of disaggregation wasn't influenced by the studied variables (p < 0.05. The best homogeneity was verified for the formulation with triple impregnation at 10%.

  10. Study of morphological alterations of the adrenal glands in the neoplastic cachexia Estudo das alterações morfológicas da glândula adrenal na caquexia neoplásica

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    Tânia Longo Mazzuco

    2009-01-01

    parameters, cachexia index and adrenal glands weight, were evaluated. Animals with tumor presented cachexia index of 16,6 ± 4%. Adrenal glands average weight was significantly higher in the tumor group (40 mg ± 10 than in the control group (25 mg ± 3. Adrenal cortex of animals with cachexia showed hypertrophy of the zona fasciculata and reticular layer, with voluminous spongiocytes; vascular congestion and stasis were observed in the medullar region. Results were similar in the pair and ad libitum-fed groups. Animals with cancer cachexia showed compromised morphology of the adrenal glands which showed alterations related to stress response, suggesting increased cathecolamine secretion and activation of the hypothalamus-pituitary-adrenal axis.   O câncer em estágio avançado cursa com alterações nutricionais e metabólicas que caracterizam o estado de caquexia neoplásica. Em situações de comprometimento da homeostasia, a glândula adrenal tem papel fundamental na resposta neuroendócrina. Para estudar as alterações morfológicas da adrenal no desenvolvimento do câncer associado à caquexia, utilizamos o modelo experimental de caquexia induzida pelo tumor de Walker-256 em ratos Wistar. Os animais foram sacrificados 12 dias após inoculação tumoral e as adrenais removidas para análise histopatológica por meio da coloração por hematoxilina-eosina. Foram avaliados os parâmetros nutricionais, índice de caquexia e peso das adrenais. Os animais com tumor apresentaram índice de caquexia de 16,6 ± 4%. A média do peso das adrenais foi significativamente maior no grupo tumor (40 mg ± 10 do que no controle (25 mg ± 3. O córtex adrenal dos animais com caquexia apresentou hipertrofia das camadas fasciculada e reticular, exibindo espongiócitos volumosos; a região medular apresentou congestão e estase vascular. Os resultados foram semelhantes nos animais do grupo pair fed e do grupo alimentado ad libitum. Animais com caquexia devido ao câncer apresentam

  11. Expressão da proteína p53 em 106 adenomas pleomórficos de glândula salivar maior p53 protein expression in 106 pleomorphic adenomas of major salivary gland

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    Jerlucia Cavalcanti das Neves

    2009-08-01

    Full Text Available INTRODUÇÃO E OBJETIVO: O adenoma pleomórfico (AP é o tumor das glândulas salivares mais comum. A parótida é o sítio anatômico mais frequente. Características clínicas e microscópicas são bem conhecidas, entretanto sua patogênese ainda é incerta, como a expressão de oncogenes e fatores que influenciam a transformação maligna. O objetivo deste estudo foi analisar e correlacionar os aspectos histopatológicos indicativos de transformação maligna de AP de glândula salivar maior, com a expressão da proteína p53 nesses tumores. MATERIAL E MÉTODO: Foram avaliados 106 casos de AP por pesquisa retrospectiva nos prontuários clínicos, revisão das preparações histológicas em hematoxilina e eosina (HE e realização de técnica imuno-histoquímica, pelo método da estreptoavidina-biotina, utilizando o anticorpo primário anti-p53. RESULTADOS: Alterações histopatológicas relacionadas com a transformação maligna foram incomuns: hialinização extensa (5; 4,7% e necrose (1; 0,9%. Houve positividade para a proteína p53 em 25 dos 106 casos estudados (23,58%. O arranjo histológico das células dos tumores considerados positivos para p53 foi principalmente dos tipos ductal (92%; 23 e cordonal (88%; 22. Não houve associação estatisticamente significante entre a positividade para o p53 e as variáveis histopatológicas, em comparação com os casos negativos: quantidade e tipo de matriz extracelular; alterações teciduais sugestivas de transformação maligna e metaplasia. CONCLUSÃO: Poucos casos que exibiram expressão para p53 apresentaram algum tipo de alteração morfológica sugestiva de malignidade, e a correlação entre a imunoexpressão da proteína p53 e a presença de alteração sugestiva de transformação maligna não foi estatisticamente significante, sugerindo que a expressão dessa proteína nesses tumores independe dessas características.INTRODUCTION AND OBJECTIVE: The pleomorphic adenoma is the most common

  12. Human cytomegalovirus gH stability and trafficking are regulated by ER-associated degradation and transmembrane architecture.

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    Gardner, Thomas J; Hernandez, Rosmel E; Noriega, Vanessa M; Tortorella, Domenico

    2016-03-30

    The prototypic betaherpesvirus human cytomegalovirus (CMV) establishes life-long persistence within its human host. While benign in healthy individuals, CMV poses a significant threat to the immune compromised, including transplant recipients and neonates. The CMV glycoprotein complex gH/gL/gO mediates infection of fibroblasts, and together with the gH/gL/UL128/130/131 a pentameric complex permits infection of epithelial, endothethial, and myeloid cells. Given the central role of the gH/gL complex during infection, we were interested in studying cellular trafficking of the gH/gL complex through generation of human cells that stably express gH and gL. When expressed alone, CMV gH and gL were degraded through the ER-associated degradation (ERAD) pathway. However, co-expression of these proteins stabilized the polypeptides and enhanced their cell-surface expression. To further define regulatory factors involved in gH/gL trafficking, a CMV gH chimera in which the gH transmembrane and cytoplasmic tail were replaced with that of human CD4 protein permitted cell surface gH expression in absence of gL. We thus demonstrate the ability of distinct cellular processes to regulate the trafficking of viral glycoproteins. Collectively, the data provide insight into the processing and trafficking requirements of CMV envelope protein complexes and provide an example of the co-opting of cellular processes by CMV.

  13. Local anesthetic lidocaine induces apoptosis in human corneal stromal cells in vitro

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    Xin Zhou

    2013-12-01

    Full Text Available AIM: To demonstrate the apoptosis-inducing effect of lidocaine on human corneal stromal (HCS cells in vitro, and provide experimental basis for safety anesthetic usage in clinic of ophthalmology.METHODS: In vitro cultured HCS cells were treated with lidocaine at different doses and times, and their morphology was monitored successively with inverted phase contrast microscopy. The membrane permeability of them was detected by acridine orange/ethidium bromide (AO/EB double staining. The DNA fragmentation of them was examined by agarose gel electrophoresis, and their ultrastructure was observed by transmission electron microscopy (TEM, respectively.RESULTS: Exposure to lidocaine at doses from 0.3125g/L to 20g/L induced morphological changes of HCS cells such as cytoplasmic vacuolation, cellular shrinkage, and turning round, and elevated membrane permeability of these cells in AO/EB staining. The change of morphology and membrane permeability was dose- and time-dependent, while lidocaine at dose below 0.15625g/L could not induce these changes. Furthermore, lidocaine induced DNA fragmentation and ultrastructural changes such as cytoplasmic vacuolation, structural disorganization, chromatin condensation, and apoptotic body appearance of the cells.CONCLUSION: Lidocaine has significant cytotoxicity on human corneal stromal cells in vitro in a dose- and time-dependent manner by inducing apoptosis of these cells. The established experimental model and findings based on this model here help provide new insight into the apoptosis-inducing effect of local anesthetics in eye clinic.

  14. Carcinoma apócrino na glândula parótida e na região submandibular Apocrine carcinoma in the parotid gland and in the submandibular region

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    Jairo S. Francisco

    2005-04-01

    Full Text Available Os objetivos deste trabalho consistem na apresentação de um caso de carcinoma apócrino e na discussão de aspectos relacionados ao seu diagnóstico, tratamento e prognóstico. Os carcinomas com diferenciação apócrina que não correspondem aos casos de doença extramamária de Paget, de carcinoma ductal de mama, de adenocarcinoma das glândulas de Moll e de carcinoma ceruminal são tumores muito raros. Relatamos o caso de uma paciente do sexo feminino, negra, com 51 anos, na qual duas lesões de carcinoma apócrino acometeram a parótida esquerda (processo inicial e recidiva e uma lesão envolveu a pele da região submandibular do mesmo lado. O exame histopatológico destas lesões mostrou a presença de neoplasia epitelial glandular infiltrativa com pleomorfismo celular e nuclear moderados; apresentando células poligonais ou arredondadas, com núcleos grandes e citoplasma eosinofílico e granular. Destacou-se a presença de secreção por decapitação apical na maior parte das células tumorais voltadas para a luz das estruturas císticas neoplásicas. Adicionalmente, foi encontrada a presença de focos de comedo-necrose e de material corado pelo PAS com e sem diastase. Apesar de não podermos definir com certeza qual a sede do tumor primário, com base nos aspectos histopatológicos compatíveis com o carcinoma apócrino cutâneo, consideramos que tenha sido, provavelmente, a lesão retirada da pele da região submandibular. A paciente foi submetida a tratamentos cirúrgicos e não apresentou alterações após um ano de acompanhamento, depois da retirada do tumor recidivante na parótida.The objectives of this paper are to report a case of apocrine carcinoma and the discussion of aspects related to its diagnosis, treatment, and prognosis. Carcinomas with apocrine differentiation not related to extramammary Paget's disease, ductal breast carcinoma, Moll's glands adenocarcinoma and ceruminous glands carcinoma are very uncommon tumors. We

  15. Activity, distribution and regulation of phosphofructokinase in salivary gland of rats with streptozotocin-induced diabetes Atividade, distribuição e regulação da fosfofrutoquinase em glândulas salivares de ratos com diabetes induzido por estreptozotocina

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    José Nicolau

    2006-04-01

    Full Text Available Although the influence of diabetes on salivary glands is well studied, it still presents conflicting results. In this work, the regulation of the phosphofructokinase-1 enzyme (PFK-1 was studied utilizing the salivary glands of rats. Diabetes was induced by a single intraperitoneal injection of streptozotocin (60 mg/Kg of body weight in rats (180-200 g. The animals were killed 30 days after the induction of diabetes and the submandibular and parotid salivary glands were used. Hyperglycemia was evaluated by blood sugar determination. The distribution of PFK-1 between the soluble and cytoskeleton fractions, the phosphate content of PFK-1, the content of fructose-2,6-bisphosphate and the activity of the PFK-2 enzyme were determined. The calculated relative glandular weight showed a higher value for the parotid gland in comparison with the control, but not for the submandibular gland. The activity of PFK-1 expressed per gland showed no variation between diabetic and control animals. However, considering the specific activity, the soluble enzyme presented a value 50% higher than that of the control and the cytoskeleton bound form increased by 84% compared to the control. For the parotid gland, no difference in the specific activity between diabetic and control animals was observed. On the other hand, the activity per gland of the soluble enzyme increased in the diabetic animals. The phosphate content of PFK-1 increased in the submandibular and parotid glands of diabetic rats. Both the content of fructose-2,6-bisphosphate and the active form of PFK-2 were reduced in the diabetic glands. In conclusion, the increase in the activity of PFK-1 observed in the salivary glands of rats with streptozotocin-induced diabetes does not seem to be due to its modulator fructose-2,6-bisphosphate.Apesar de existirem muitos estudos sobre a influência do diabetes nas glândulas salivares, esses apresentam resultados conflitantes. Neste estudo, a regulação da enzima

  16. Estudo da glândula pineal de suíno por meio de microscopia de luz - DOI: 10.4025/actascibiolsci.v25i2.2038 Study of the swine pineal gland by means of light microscopy

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    Kleber Fernando Pereira

    2003-04-01

    Full Text Available Foram coletadas dez glândulas pineais de suínos da raça Landrace com 180 dias de idade, sendo cinco machos e cinco fêmeas. Após a coleta, o material foi fixado em solução de formalina a 10% por um período de 48 horas e, em seguida, submetido a tratamento de rotina para inclusão em parafina e realização de cortes histológicos, que foram corados por hematoxilina-eosina e Sírius-Red F3BA. As lâminas selecionadas foram fotografadas em fotomicroscópio do Departamento de Ciências Morfofisiológicas da Universidade Estadual de Maringá. O presente estudo teve por objetivo verificar a morfologia da glândula pineal de suínos por meio de microscopia de luz. Os resultados permitem verificar que a glândula pineal de suínos apresenta-se revestida pela pia-máter que emite projeções para o interior da glândula, constituindo septos de tecido conjuntivo. A distribuição dos elementos celulares no parênquima da glândula pineal apresenta-se de maneira heterogênea, na qual se observam regiões com escassez celular e predominância de pequenos feixes de fibras conjuntivas e concreções calcárias. Os resultados permitem concluir que a glândula pineal de suínos apresenta-se delimitada por septações de tecido conjuntivo proveniente da pia-máter, e que no interior da glândula é comum a presença de concreções calcáriasTen swine pineal glands from Landrace breed at the age of 180 days were collected, 5 males and 5 females. After the collection, the material was fastened in formaline solution at 10% for a period of 48 hours, and soon after it was undergone a routine treatment for inclusion in paraffin and accomplishment of histologic courts that have been red-faced for hematoxylin-eosin and Sírius-Red F3BA. The selected sheets were photographed in fotomicroscopy from the Department of Morphologic Sciences of Universidade Estadual de Maringá (State University of Maringá. The present study aimed to verify the morphology of swine

  17. More Human than Human.

    Science.gov (United States)

    Lawrence, David

    2017-07-01

    Within the literature surrounding nonhuman animals on the one hand and cognitively disabled humans on the other, there is much discussion of where beings that do not satisfy the criteria for personhood fit in our moral deliberations. In the future, we may face a different but related problem: that we might create (or cause the creation of) beings that not only satisfy but exceed these criteria. The question becomes whether these are minimal criteria, or hierarchical, such that those who fulfill them to greater degree should be afforded greater consideration. This article questions the validity and necessity of drawing divisions among beings that satisfy the minimum requirements for personhood; considering how future beings-intelligent androids, synthezoids, even alternate-substrate sentiences-might fit alongside the "baseline" human. I ask whether these alternate beings ought to be considered different to us, and why this may or may not matter in terms of a notion of "human community." The film Blade Runner, concerned in large part with humanity and its key synthezoid antagonist Roy Batty, forms a framing touchstone for my discussion. Batty is stronger, faster, more resilient, and more intelligent than Homo sapiens. His exploits, far beyond the capability of normal humans, are contrasted with his frailty and transient lifespan, his aesthetic appreciation of the sights he has seen, and his burgeoning empathy. Not for nothing does his creator within the mythos term him "more human than human."

  18. Nutritional and immunological characteristics of fresh and refrigerated stored human milk in Hong Kong: a pilot study.

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    Yuen, John W M; Loke, Alice Yuen; Gohel, Mayur-Danny I

    2012-10-09

    The nutritional and immunological qualities of human milk from Hong Kong mothers were profiled. A total of 25 colostrum (≤ 3 days postpartum) and 11 mature (30-45 days postpartum) milk specimens were collected from healthy Chinese women in Hong Kong. Parameters including total protein, whey, casein, triglycerides, lactose, lysozyme, secretory immunoglobulin A, lactoferrin and antibacterial activity of human milk were quantified. Breast milk of Hong Kong Chinese mothers is nutritionally comparable to that of western mothers for colostrum and mature milk, with protein (25.0±11.3 g/l vs 10.1±1.4 g/l) and lactose (44.2±7.5 g/l vs 66.6±5.5 g/l) for growth and energy supply in infants, respectively. The milk of Hong Kong mothers is however characterized by its exceptionally high levels of sIgA (806.3±792.6 mg/dl and 1545.9±334.6 mg/dl) and low levels of triglycerides (10.8±9.3 mmol/l and 11.1±7.7 mmol/l). The human milk in Hong Kong was also shown to be superior to bovine formula milk, particularly in terms of lower total protein level, the high whey to casein protein ratio, rich immunological active contents, and significant antibacterial activity against 2 common Escherichia coli strains. Preliminary data also indicated that mature milk could be refrigerated for at least 3 days in order to maintain the major nutritional constituents and antibacterial activity. Copyright © 2012 Elsevier B.V. All rights reserved.

  19. Mucoepidermoid carcinoma of the salivary glands in Brazil: clinicopathological outcomes and a brief review Carcinoma mucoepidermoide de las glándulas salivares en Brasil: resultados clinicopatológicos y una breve revisión

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    Lucinei Roberto Oliveira

    2012-06-01

    óstico de carcinoma mucoepidermoide primario de cabeza y cuello. Se realizó un estudio retrospectivo de 16 pacientes tratados entre 1990 y 2008 en el Hospital General de Ribeirao Preto, Escuela de Medicina USP, Brasil. Se estudiaron las variables: edad, sexo, localización anatómica, tamaño del tumor, estadio clínico, grado histológico, recidiva, metástasis, bordes quirúrgicos comprometidos y tratamiento, sobre los resultados clínico-patológicos. Las curvas de supervivencia fueron construidas utilizando el método de Kaplan-Meier y el análisis estadístico fue realizado mediante la prueba del log-rank. Se constató 68,7 % de pacientes del sexo masculino, todos los pacientes comprendidos en las edades entre 13 y 83 años. El 75 % de los tumores se localizó en las glándulas salivales mayores, 56,3 % en parótida, los carcinomas mucoepidermoides de bajo grado y estadio II con 37,5 %. La resección quirúrgica fue realizada en todos los pacientes. El período de seguimiento en este estudio varió entre 6 y 217 meses. La tasa general de supervivencia, tanto a los 5 como a los 10 años fue de 85,6 %, mientras que las tasas de supervivencia libre de enfermedad fueron de 81,8 % a los 5 años y de 68,2 % a los 10 años. Se demostró la existencia de influencias estadísticamente significativas del tamaño del tumor (p = 0,05, presencia de metástasis (p = 0,04, y de la localización anatómica primaria (p = 0,04 sobre las tasas de supervivencia libre de enfermedad. Los resultados obtenidos demuestran la importancia de la localización anatómica primaria del tumor, de su tamaño y de la presencia de metástasis, en la supervivencia de los carcinomas mucoepidermoides.

  20. Perfil metabólico e desenvolvimento da glândula mamária de novilhas leiteiras sob manejo para crescimento compensatório Metabolic profile and mammary gland development of dairy heifers under compensatory growth regime

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    Patrícia Barcellos Costa

    2012-02-01

    Full Text Available Objetivou-se avaliar o efeito do manejo para crescimento compensatório e da suplementação com ionóforo sobre o perfil metabólico e o crescimento da glândula mamária de novilhas leiteiras. Foram utilizadas 20 novilhas Pardo-Suíças puras, com peso inicial de 200 kg e 5 meses de idade. Os tratamentos foram arranjados em esquema fatorial 2 × 2 e os animais foram alocados aleatoriamente em cada uma das combinações. O fator 1 consistiu dos sistemas de alimentação (convencional e crescimento compensatório e o fator 2, da utilização ou não de ionóforo (200 mg de monensina/animal/dia. Os animais do sistema convencional receberam alimentação ad libitum, enquanto os do sistema para crescimento compensatório foram submetidos a um manejo nutricional com dois períodos distintos: período de restrição energética (90 dias, P1 e período de realimentação (60 dias, P2. Novilhas submetidas ao sistema crescimento compensatório apresentaram maior concentração de nitrogênio ureico (N-ureico plasmático e menor razão DNA:RNA no tecido mamário. Quando analisados dentro de período, os níveis de lipoproteínas de alta densidade (HDL aumentaram no período de realimentação, enquanto os de N-ureico diminuíram. Além da alteração nos níveis de N-ureico, o balanço energético ao qual as novilhas em crescimento compensatório foram submetidas ocasionou aumento no teor dos ácidos graxos não-esterificados. Houve redução nos níveis de glicose sanguínea dos animais em crescimento compensatório no período de realimentação. A suplementação com ionóforo proporciona menor concentração de ácidos graxos não-esterificados durante o período de realimentação.The objective of this trial was to compare the effect of compensatory growth regime on the metabolic profile and mammary gland development of dairy heifers. Twenty Brown-Swiss heifers averaging 200 kg of body weight and five months of age were used in a completely

  1. Allergenicity of milk of different animal species in relation to human milk.

    Science.gov (United States)

    Pastuszka, Robert; Barłowska, Joanna; Litwińczuk, Zygmunt

    2016-12-31

    Protein content in cow milk (with over 20 proteins, and peptides may also occur as a result of enzymatic hydrolysis) ranges from 2.5% to 4.2% and is about 1.5-2 times higher than in human milk. Its most important allergens are considered to be β-lactoglobulin (absent in human milk) and αs1-casein. The most similar in composition to human milk is horse and donkey milk. It contains considerably more whey proteins (35-50%) than cow milk (about 20%), and the concentration of the most allergenic casein fraction αs1 is 1.5-2.5 g/l. In comparison, the content of αs1-casein in cow milk is about 10 g/l. β-lactoglobulin present in donkey milk is a monomer, while in milk of ruminants it is a dimer. Like human milk, it contains a substantial amount of lactose (about 7%), which determines its flavour and facilitates calcium absorption. The high lysozyme content (about 1 g/l) gives it antibacterial properties (compared to trace amounts in ruminants). Camel milk is also more digestible and induces fewer allergic reactions, because it lacks β-lactoglobulin, and its β-casein has a different structure. It also contains (compared to cow milk) more antibacterial substances such as lysozyme, lactoferrin and immunoglobulins, and furthermore the number of immunoglobulins is compatible with human ones. Goat milk components have a higher degree of assimilability as compared to cow milk. Its main protein is β-casein, with total protein content depending on the αs1-casein genetic variant. Goats with the '0' variant do not synthesize this allergenic protein. Clinical and immunochemical studies indicate, however, that it cannot be a substitute for cow milk without the risk of an anaphylactic reaction.

  2. Allergenicity of milk of different animal species in relation to human milk

    Directory of Open Access Journals (Sweden)

    Robert Pastuszka

    2016-12-01

    Full Text Available Protein content in cow milk (with over 20 proteins, and peptides may also occur as a result of enzymatic hydrolysis ranges from 2.5% to 4.2% and is about 1.5-2 times higher than in human milk. Its most important allergens are considered to be β-lactoglobulin (absent in human milk and αs1-casein. The most similar in composition to human milk is horse and donkey milk. It contains considerably more whey proteins (35-50% than cow milk (about 20%, and the concentration of the most allergenic casein fraction αs1 is 1.5-2.5 g/l. In comparison, the content of αs1-casein in cow milk is about 10 g/l. β-lactoglobulin present in donkey milk is a monomer, while in milk of ruminants it is a dimer. Like human milk, it contains a substantial amount of lactose (about 7%, which determines its flavour and facilitates calcium absorption. The high lysozyme content (about 1 g/l gives it antibacterial properties (compared to trace amounts in ruminants. Camel milk is also more digestible and induces fewer allergic reactions, because it lacks β-lactoglobulin, and its β-casein has a different structure. It also contains (compared to cow milk more antibacterial substances such as lysozyme, lactoferrin and immunoglobulins, and furthermore the number of immunoglobulins is compatible with human ones. Goat milk components have a higher degree of assimilability as compared to cow milk. Its main protein is β-casein, with total protein content depending on the αs1-casein genetic variant. Goats with the ‘0’ variant do not synthesize this allergenic protein. Clinical and immunochemical studies indicate, however, that it cannot be a substitute for cow milk without the risk of an anaphylactic reaction.

  3. Concentración de anticuerpos contra proteínas de las glándulas salivales de Aedes aegypti e historia de la exposición al virus del dengue en residentes de una zona endémica colombiana

    Directory of Open Access Journals (Sweden)

    Berlin Londoño-Rentería

    2015-12-01

    Full Text Available Introducción. Las proteínas salivales de los mosquitos son capaces de inducir la producción de anticuerpos, lo que a su vez refleja el grado de contacto hombre-vector. Además, los anticuerpos IgG contra el virus del dengue son indicadores de una exposición previa a este virus. El riesgo de transmisión del virus del dengue está asociado no solo con factores relacionados con la biología del mosquito, o factores virales, sino también, con factores socioeconómicos, como la disponibilidad de agua en el hogar, que pueden desempeñar un papel importante durante la temporada epidémica. Objetivo. Determinar el efecto de la presencia de mosquitos Aedes aegypti en las casas y la exposición previa al virus del dengue, sobre los niveles de anticuerpos contra mosquitos en el contacto humano-vector en habitantes de un área endémica de Colombia. Materiales y métodos. Se hizo un estudio piloto de 58 casas y 55 participantes en Norte de Santander, Colombia. Se empleó un cuestionario para recopilar la información sobre los factores socioeconómicos y se examinaron las casas para detectar la presencia de sitios de cría de Ae. aegypti. Se recolectó una muestra de sangre humana total en papel de filtro y se estableció el nivel de anticuerpos IgG contra el virus del dengue, además del de los anticuerpos IgG e IgM anti-Ae. aegypti de extracto de glándula salival mediante ELISA. Resultados. Los resultados revelaron un mayor nivel de anticuerpos IgG de extracto de glándula salival en sujetos que vivían en casas con presencia de mosquitos Ae. aegypti en la fase acuática. Asimismo, se encontró una mayor concentración de anticuerpos IgG de extracto de glándula salival en personas previamente expuestas al virus del dengue. Los resultados evidenciaron una correlación positiva significativa entre los niveles de IgM de extracto de glándula salival y los de IgG anti-virus del dengue de extracto de glándula salival, y una correlación negativa con

  4. Canine lacrimal and third eyelid superficial glands’ macroscopic and morphometric characteristics Aspectos macroscópicos e morfométricos das glândulas lacrimal e superficial da terceira pálpebra de cães (Canis familiares; LINNAEUS, 1758

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    Vânia Pais Cabral

    2005-04-01

    Full Text Available The lacrimal and third eyelid superficial glands produce the aqueous component of the preocular tear film. In this research, morphologic and morphometric assessments of the parenchyma and stroma of both lacrimal glands of healthy adult mongrel dogs were performed. Both lacrimal and third eyelid glands of fourteen dogs were collected, summing fifty-six samples. The macroscopic and morphometric data were statistically analyzed, according to the glandular type (lacrimal and third eyelid superficial glands and sexual dimorphism (male or female. The lacrimal glands were significantly larger and longer than the superficial glands of the third eyelid. Expressive morphometric differences of interlobular duct, lymphocytic infiltration, interlobular vessels and secretory parenchyma between the two glandular types were encountered. The lacrimal glands from the male subjects were significantly larger than those from female ones, as well as the superficial glands of the third eyelid were thicker. The higher lymphocyte infiltration and poorer secretor parenchyma in female dogs may be one of the reasons for the higher incidence of keratoconjunctiviti sicca (KCS in such canine population.As glândulas lacrimal e superficial da terceira pálpebra atuam produzindo o componente aquoso do filme lacrimal. Nesta pesquisa, estudaram-se aspectos morfológicos e morfométricos do parênquima e do estroma de ambas as glândulas em cães mestiços, hígidos, adultos (machos ou fêmeas. As glândulas lacrimal e superficial da terceira pálpebra de 14 cães foram colhidas, totalizando 56 amostras. Foram estudadas, à estatística, as variáveis macroscópicas e morfométricas, comparando-as quanto ao tipo glandular (lacrimal e superficial da terceira pálpebra e quanto ao dimorfismo sexual (macho e fêmea. Às glândulas lacrimais foram significativamente maiores comparativamente as superficiais da terceira pálpebra. Foram evidenciados diferenças morfom

  5. A retrospective analysis of 25% human serum albumin supplementation in hypoalbuminemic dogs with septic peritonitis

    Science.gov (United States)

    Horowitz, Farrah B.; Read, Robyn L.; Powell, Lisa L.

    2015-01-01

    This study describes the influence of 25% human serum albumin (HSA) supplementation on serum albumin level, total protein (TP), colloid osmotic pressure (COP), hospital stay, and survival in dogs with septic peritonitis. Records of 39 dogs with septic peritonitis were evaluated. In the HSA group, initial and post-transfusion TP, albumin, COP, and HSA dose were recorded. In the non-supplemented group, repeated values of TP, albumin, and COP were recorded over their hospitalization. Eighteen dogs survived (53.8% mortality). Repeat albumin values were higher in survivors (mean 23.9 g/L) and elevated repeat albumin values were associated with HSA supplementation. Repeat albumin and TP were higher in the HSA supplemented group (mean 24 g/L and 51.9 g/L, respectively) and their COP increased by 5.8 mmHg. Length of hospitalization was not affected. Twenty-five percent HSA increases albumin, TP, and COP in canine patients with septic peritonitis. Higher postoperative albumin levels are associated with survival. PMID:26028681

  6. Absorção de água, solubilidade em água, propriedades mecânicas e morfológicas de compósitos de glúten de milho e poli(hidroxibutirato-co-valerato (PHBV reforçados com fibras de coco verde

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    Elisângela Corradini

    2013-01-01

    Full Text Available Neste estudo, fibras de coco verde foram incorporadas em uma matriz composta de glúten de milho (GM e poli(hidroxibutirato-co-valerato (PHBV. As composições foram processadas por extrusão, onde o teor máximo de glúten adicionado à matriz para obter misturas com viscosidade suficientemente baixa para o processamento foi de 50% (em peso em relação ao teor de PHBV. O teor de fibras nos compósitos variou de 5 a 20% por peso em relação ao peso total dos polímeros. Os plastificantes usados foram acetil tributil citrato (ATBC e glicerol. Os materiais extrudados foram moldados por compressão a quente para obtenção de corpos de prova para a caracterização das propriedades de absorção de água, solubilidade em água, resistência mecânica, além de propriedades termo-dinâmico-mecânicas (DMTA e morfológicas. Foi observado que as matrizes apresentaram duas transições vítreas (Tg's uma para cada componente, indicando que as mesmas são compostas de duas fases (GM e PHBV. A adição do PHBV ao glúten favoreceu ao aumento da rigidez e houve diminuições significativas na absorção de água e na solubilidade em água, enquanto a presença das fibras provocou, em algumas composições, um aumento no módulo de elasticidade e na resistência à tração.

  7. Estrutura macro e microscópica das glândulas salivares parótidas em duas espécies de procionídeos: mão-pelada (Procyon cancrivorus, G. Cuvier, 1798 e quati (Nasua nasua, Linnaeus, 1766

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    Amilton Cesar dos Santos

    2011-11-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2012v25n1p93 O quati (Nasua nasua, Linnaeus, 1766, de hábito diurno, e o mão-pelada (Procyon cancrivorus, G. Cuvier, 1798, de hábito crepuscular e noturno, são espécies de mamíferos terrestres, pertencentes à família dos procionídeos, que são amplamente encontrados no território brasileiro. Para este trabalho foram utilizados três quatis e dois mãos-peladas adultos provenientes do Criatório Científico-Cecrimpas do Centro Universitário da Fundação de Ensino Octávio Bastos (Ibama 02027.003731/04-76 dos quais foram obtidas amostras de tecido das glândulas salivares parótidas coradas em H.E. para microscopia de luz. Constatou-se que a glândula parótida dos quatis e mãos-peladas possui posicionamento semelhante à dos demais carnívoros domésticos, com seus ductos se abrindo no vestíbulo oral, próximo à altura do quarto dente pré-molar superior, e que histologicamente as glândulas parótidas dos quatis e mão-peladas são constituídas por ácinos serosos, assemelhando-se à maioria dos animais domésticos, homens e roedores e diferindo dos resultados encontrados nos cães jovens, cordeiros e outros carnívoros como o furão que possuem ácinos mistos, e de alguns carnívoros, que apresentam ácinos serosos e mucosos. Conclui-se que o fato da constituição serosa dos ácinos das glândulas parótidas dos quatis e mão-peladas se assemelhar pode ser devido ao hábito alimentar onívoro compartilhado pelas duas espécies.

  8. IT-säkerhet och människan : De har världens starkaste mur men porten står alltid på glänt

    OpenAIRE

    Wendel-Persson, Fredrik; Ronnhed, Anna

    2017-01-01

    In the ever-changing environment of the digital world one thing remains the same, the humans sitting in front of the screen. Today protecting company secrets and information is becoming more and more vital and companies invest massive amounts of money on technological defenses such as firewalls and antivirus programs, but the threats that the user and employees pose for companies go unnoticed by many. However, there are people that notice this weakness in companies’ security and try to take a...

  9. Quantification of lactose content in human and cow's milk using UPLC-tandem mass spectrometry.

    Science.gov (United States)

    Fusch, Gerhard; Choi, Arum; Rochow, Niels; Fusch, Christoph

    2011-12-01

    A sensitive, accurate, and specific quantitative UPLC-MS/MS method was developed for lactose measurement of cow's and human milk and validated with cow's milk samples certified by an external laboratory. The new method employs only a dilution of raw cow's and human milk for simple preparation with no need to remove protein and fat prior to analysis with UPLC-MS/MS. It was operated in negative mode to detect lactose molecules and labeled (13)C(12)-lactose with the highest sensitivity. The principle advantages of the new LC-MS/MS method were: completed lactose determination in 5 min, absolute recovery of 97-107%, lower limit of detection cow's and human milk. The mean lactose concentration of 51 human milk samples was measured as 56.8 ± 5.5 g/L ranging from 43 to 65 g/L. The described method represents validated lactose analysis with high accuracy and precision for a routine lactose determination in raw human milk. Crown Copyright © 2011. Published by Elsevier B.V. All rights reserved.

  10. Human-Friendly Light-Emitting Diode Source Stimulates Broiler Growth.

    Science.gov (United States)

    Pan, Jinming; Yang, Yefeng; Yang, Bo; Dai, Wenhua; Yu, Yonghua

    2015-01-01

    Previous study and our laboratory have reported that short-wavelength (blue and green) light and combination stimulate broiler growth. However, short-wavelength stimuli could have negative effects on poultry husbandry workers. The present study was conducted to evaluate the effects of human-friendly yellow LED light, which is acceptable to humans and close to green light, on broiler growth. We also aimed to investigate the potential quantitative relationship between the wavelengths of light used for artificial illumination and growth parameters in broilers. After hatching, 360 female chicks ("Meihuang" were evenly divided into six lighting treatment groups: white LED strips (400-700 nm, WL); red LED strips (620 nm, RL); yellow LED strips (580 nm, YL); green LED strips (514 nm, GL); blue LED strips (455 nm, BL); and fluorescent strips (400-700 nm, FL). From 30 to 72 days of age, broilers reared under YL and GL were heavier than broilers treated with FL (P 0.05). Moreover, YL significantly improved feeding efficiency when compared with GL and BL at 45 and 60 days of age (P light spectra on broiler growth and a quantitative relationship between LED light spectra (455 to 620 nm) and the live body weights of broilers. The wavelength of light (455 to 620 nm) was found to be negatively related (R2 = 0.876) to live body weight at an early stage of development, whereas the wavelength of light (455 to 620 nm) was found to be positively correlated with live body weight (R2 = 0.925) in older chickens. Our results demonstrated that human-friendly yellow LED light (YL), which is friendly to the human, can be applied to the broilers production.

  11. Coeficientes de Gini locacionais - GL: aplicação à indústria de calçados do Estado de São Paulo Locational Gini coefficients - LG: application to the shoe industry in the state of São Paulo

    Directory of Open Access Journals (Sweden)

    Wilson Suzigan

    2003-01-01

    Full Text Available This paper suggests a specific methodology to geographically locate and delimit local production systems, with an application to the leather and shoe industry in the state of São Paulo. The methodology consists on the elaboration of locational Gini coefficients (GL for four-digit industries at municipal or micro region level from data in RAIS/MTE and PIA/IBGE. These GL coefficients indicate the industrial branches which are spatially concentrated. For the latter, location quotients (QL by microregions in the state are used in order to identify local production systems within these industries. The QL data - combined with data on the share of the microregion in question in total industry employment and number of industrial plants - unequivocally show that there are three main local production systems in the leather and shoe industry in the state of São Paulo: Franca, Birigui, and Jaú. Based on a field research work, these three local systems are briefly characterized in regard to their location, territorial extension, production structure and integration, local institutions, and forms of interfirm cooperation.

  12. Isolation of rabies virus from the parotid salivary glands of foxes (Pseudalopex vetulus from Paraíba State, Northeastern Brazil Isolamento de vírus rábico de glândulas salivares parótidas de raposas (Pseudalopex vetulus do Estado da Paraíba, Nordeste do Brasil

    Directory of Open Access Journals (Sweden)

    Maria Luana Cristiny Rodrigues Silva

    2009-09-01

    Full Text Available To determine the presence of rabies virus in the parotid salivary glands, 12 road-killed rabies-positive hoary foxes (Pseudoalopex vetulus were tested by using the fluorescent antibody test (FAT and mouse inoculation test (MIT. All 12 parotid salivary glands were positive for both tests, although in some cases several passages were required. The findings of this study support the importance of the hoary fox as rabies reservoir in the semi-arid region of Paraíba State, Northeastern Brazil.Para determinar a presença de vírus rábico em glândulas salivares parótidas, 12 raposas (Pseudalopex vetulus atropeladas em rodovias e positivas para raiva foram testadas pelo teste de imunofluorescência direta (IFD e teste de inoculação em camundongos (IC. Todas as 12 glândulas salivares parótidas foram positivas em ambos os testes, embora, em alguns casos, várias passagens terem sido necessárias. Os achados do presente trabalho reforçam a importância das raposas como reservatórios de raiva no semi-árido do Estado da Paraíba, Nordeste do Brasil.

  13. The anti-Müllerian hormone (AMH) acts as a gatekeeper of ovarian steroidogenesis inhibiting the granulosa cell response to both FSH and LH.

    Science.gov (United States)

    Sacchi, Sandro; D'Ippolito, Giovanni; Sena, Paola; Marsella, Tiziana; Tagliasacchi, Daniela; Maggi, Elena; Argento, Cindy; Tirelli, Alessandra; Giulini, Simone; La Marca, Antonio

    2016-01-01

    Anti Müllerian Hormone (AMH) has a negative and inhibitory role in many functions of human granulosa-lutein cells (hGCs) including notoriously the reduction of the aromatase CYP19A1 expression induced by follicle-stimulating hormone (FSH). No data have been provided on the possible role of AMH in modulating the response to luteinizing hormone (LH) (alone or combined with FSH) as well as its effect on other enzymes involved in steroidogenesis including aromatase P450scc. The aim of this study was to investigate the role of AMH as regulator of the basal and stimulated steroids production by hGCs. Primary culture of hGCs were incubated with hormones AMH, LH, and FSH, alone or in combination. The CYP19A1 and P450scc messenger RNA (mRNA) expression, normalized by housekeeping ribosomal protein S7 (RpS7) gene, was evaluated by reverse transcriptase quantitative PCR (RT-qPCR). Each reaction was repeated in triplicate. Negative controls using corresponding amount of vehicle control for each hormone treatment were performed. AMH did not modulate the basal mRNA expression of both aromatase genes at any of the concentrations tested. Meanwhile, the strong mRNA induction of CYP19A1 and P450scc generated by a 24-h gonadotropin treatment (alone and combined) was suppressed by 20 ng/ml AMH added to culture medium. These findings contribute in clarifying the relationship between hormones regulating the early phase of steroidogenesis confirming that AMH is playing a suppressive role on CYP19A1 expression stimulated by gonadotropin in hGCs. Furthermore, a similar inhibitory effect for AMH was observed on P450scc gene expression when activated by gonadotropin treatment.

  14. Estudo histológico e histoquímico da glândula de Duvernoy de Clelia plumbea (Wied (Serpentes, Colubridae, Xenodontinae Histological and histochemical study of Duvernoy's gland from Clelia plumbea (Wied (Serpentes, Colubridae, Xenodontinae

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    Márcia Ferret Renner

    2000-09-01

    Full Text Available There are report two human envenenomations for species from Clelia Fitzinger, 1826 (opisthoglyph snake. The patients exhibited symptoms similar from bothropic accident. Then this work have with objective, the hystological and histochemical study of Duvernoy's gland from Clelia plumbea. For the hystological observation of the Duvernoy's gland were using, with paraffin's cutting edge, two methods: Hematoxylin + Eosin and Toluidine's Blue. With historesine's cutting edge were using three methods: Metilen's Blue, Toluidine's Blue + Floxine and Toluidine's Blue. For the histochemical detection were using six techniques, with paraffin's cutting edge: PAS, PAS + Alcian Blue pH 2,5, Alcian Blue pH 2,5, PAS + Salivar Amilase, Method of Lars Grimelius and Method of Lillie. It was evidenced the predominance of serous cells on Duvernoy's gland. In relation to the histochemical constituion of the duvernoy's gland, was verified the presence of glucoconjugates neuters, enzymes and cells with reduetive activity. By fact from this species to kill his preys by constriction, the predominance of serous cells in the duvernoy's gland, don' t must be direct related with the subjugation of his preys, but with the digestive processes. Histological and histochemical analysis showed cells of Duvernoy's gland are constituted for two populations: serous cells (prodution of enzymes and mucous cells (prodution of glucoconjugates.

  15. Effects of olsalazine and sulphasalazine on jejunal and ileal water and electrolyte absorption in normal human subjects.

    OpenAIRE

    Raimundo, A H; Patil, D H; Frost, P G; Silk, D B

    1991-01-01

    The effect of sulphasalazine and olsalazine on jejunal and ileal water and electrolyte absorption was investigated in normal subjects by a steady state intestinal perfusion of a physiological glucose bicarbonate electrolyte solution in the absence and presence of increasing concentrations of each drug. (Olsalazine 0.25 g/l, 1.0 g/l, jejunum; 0.5 g/l, 1.0 g/l, ileum; sulphasalazine 0.25 g/l, 0.5 g/l, 2.0 g/l jejunum; 1.0 g/l, 2.0 g/l, ileum.) In the jejunum olsalazine at 1.0 g/l significantly ...

  16. Human rights

    NARCIS (Netherlands)

    Gaay Fortman, B. de

    2006-01-01

    Human rights reflect a determined effort to protect the dignity of each and every human being against abuse of power. This endeavour is as old as human history. What is relatively new is the international venture for the protection of human dignity through internationally accepted legal standards

  17. [Variations in the concentration of total human milk proteins in the first month of lactation].

    Science.gov (United States)

    Mladenović, Marija; Radlović, Nedeljko; Leković, Zoran; Ristić, Dragana; Radlović, Petar; Gajić, Milan; Djurdjević, Jelena

    2007-01-01

    Human milk proteins are maximally adapted to physiological needs of a neonate. Thus, depending on the speed of the neonatal growth and development, the content of milk proteins changes, both in quantity and quality. The study was conducted in order to determine variations of total protein concentrations in milk in the first and third lactation week in lactating mothers of term and preterm neonates. Also, we analyzed the influence of the mode of delivery, neonatal Apgar score and parity on the concentration of human milk proteins in both lactation phases. The study aims were evaluated on the sample of 48 women, of whom 33 were mothers of term neonates and 15 of neonates born between the 34th to 37th gestational weeks. Total protein level of the lactation milk from the middle phase was determined using the standard laboratory method (Lowry et al., 1951), and the obtained differences were analyzed by t-test. Total protein concentration in term colostrum was 17.60-45.17 g/l (X = 24.71 +/- 5.19), while in preterm colostrum it was 28.39-73.30 g/l (X = 39.17 +/- 11.08). The total protein level of mature milk in women who had term delivery was 11.90-22.11 g/l (X = 16.39 +/- 2.96), while in women who had preterm delivery it was 14.50-44.19 g/l (X = 23.25 +/-8.96). The obtained results indicated that total protein concentration in women who had preterm delivery was significantly higher than that of women who had term delivery, both in the colostral and mature phase of lactation. (p protein concentration was statistically highly significant (p total protein level of human milk were not significant, depending on the prematurity stage, the mode and severity of delivery and parity, both in the first and third week of lactation. Our results show that total protein concentration in human milk was significantly higher in the first than the third week of lactation. In both lactation phases, milk protein content was higher in women who had preterm delivery than those having had term

  18. Variations in the concentration of total human milk proteins in the first month of lactation

    Directory of Open Access Journals (Sweden)

    Mladenović Marija

    2007-01-01

    Full Text Available Introduction. Human milk proteins are maximally adapted to physiological needs of a neonate. Thus, depending on the speed of the neonatal growth and development, the content of milk proteins changes, both in quantity and quality. Objective. The study was conducted in order to determine variations of total protein concentrations in milk in the first and third lactation week in lactating mothers of term and preterm neonates. Also, we analyzed the influence of the mode of delivery, neonatal Apgar score and parity on the concentration of human milk proteins in both lactation phases. Method. The study aims were evaluated on the sample of 48 women, of whom 33 were mothers of term neonates and 15 of neonates born between the 34th to 37th gestational weeks. Total protein level of the lactation milk from the middle phase was determined using the standard laboratory method (Lowry et al., 1951, and the obtained differences were analyzed by t-test. Results. Total protein concentration in term colostrum was 17.60-45.17 g/l (X=24.71±5.19, while in preterm colostrum it was 28.39-73.30 g/l (X=39.17±11.08. The total protein level of mature milk in women who had term delivery was 11.90-22.11 g/l (X=16.39±2.96, while in women who had preterm delivery it was 14.50-44.19 g/l (X=23.25±8.96. The obtained results indicated that total protein concentration in women who had preterm delivery was significantly higher than that of women who had term delivery, both in the colostral and mature phase of lactation. (p<0.01. Also, the difference in the protein concentration was statistically highly significant (p<0.01 in the colostral and mature phase of lactation, both in women who had term and preterm delivery. Variations in the total protein level of human milk were not significant, depending on the prematurity stage, the mode and severity of delivery and parity, both in the first and third week of lactation. Conclusion. Our results show that total protein concentration

  19. High-level secretion of native recombinant human calreticulin in yeast

    DEFF Research Database (Denmark)

    Čiplys, Evaldas; Žitkus, Eimantas; Gold, Leslie I.

    2015-01-01

    , Saccharomyces cerevisiae and Pichia pastoris. RESULTS: Expression of a full-length human CRT precursor including its native signal sequence resulted in high-level secretion of mature recombinant protein into the culture medium by both S. cerevisiae and P. pastoris. To ensure the structural and functional...... recombinant CRT protein with yields reaching 75 % of total secreted protein and with production levels of 60 and 200 mg/l from S. cerevisiae and P. pastoris, respectively. Finally, cultivation of P. pastoris in a bioreactor yielded CRT secretion titer to exceed 1.5 g/l of culture medium. CONCLUSIONS: Yeasts...

  20. Human Rights, Human Needs, Human Development, Human Security

    OpenAIRE

    Gasper, D.R.

    2007-01-01

    Human rights, human development and human security form increasingly important, partly interconnected, partly competitive and misunderstood ethical and policy discourses. Each tries to humanize a pre-existing and unavoidable major discourse of everyday life, policy and politics; each has emerged within the United Nations world; each relies implicitly on a conceptualisation of human need; each has specific strengths. Yet mutual communication, understanding and co-operation are deficient, espec...

  1. Role of Proteins and of Some Bioactive Peptides on the Nutritional Quality of Donkey Milk and Their Impact on Human Health

    Directory of Open Access Journals (Sweden)

    Silvia Vincenzetti

    2017-07-01

    Full Text Available Donkey milk could be considered a good and safer alternative, compared to other types of milk, for infants affected by cow’s milk protein allergy, when breastfeeding is not possible. Interestingly, donkey milk has low allergenicity, mainly due to the low total casein amount, and the content of some whey proteins that act as bioactive peptides. The amount of lysozyme, an antibacterial agent, is 1.0 g/L, similar to human milk. Lactoferrin content is 0.08 g/L, with this protein being involved in the regulation of iron homoeostasis, anti-microbial and anti-viral functions, and protection against cancer development. Lactoperoxidase, another protein with antibacterial function, is present in donkey milk, but in very low quantities (0.11 mg/L. β-lactoglobulin content in donkey milk is 3.75 g/L—this protein is able to bind and transport several hydrophobic molecules. Donkey milk’s α-lactalbumin concentration is 1.8 g/L, very close to that of human milk. α-lactalbumin shows antiviral, antitumor, and anti-stress properties. Therefore, donkey milk can be considered as a set of nutraceuticals properties and a beverage suitable, not only for the growing infants, but for all ages, especially for convalescents and for the elderly.

  2. Análise da voz e do comportamento do trato vocal suplaglótico por meio visual, perceptivo-auditivo e acústico em mulheres disfônicas com diferentes configurações glóticas

    OpenAIRE

    Nunes, Raquel Buzelin

    2005-01-01

    Objetivo: análise do comportamento do trato vocal supraglótico, por meio de avaliação visual da imagem do trato, perceptivo-auditiva e acústica da voz em mulheres disfônicas, com diferentes configurações glóticas. Método: O estudo constituiu-se de 31 mulheres, faixa etária entre 20 e 45 anos, com queixa e alteração vocal. Os indivíduos foram submetidos à avaliação resumida do sistema sensório-motor-oral, exames de videolaringoestroboscopia, nasofibrolaringoscopia e gravação da voz. Em um prim...

  3. Adaptações do músculo glúteo médio em eqüinos submetidos a treinamento de resistência e suplementados com diferentes concentrações de óleo de soja

    OpenAIRE

    Martins, Carla Braga [UNESP

    2007-01-01

    Objetivou-se avaliar o efeito da suplementação com diferentes concentrações de óleo de soja e do treinamento de resistência nas adaptações do músculo glúteo médio de 20 eqüinos da raça Puro Sangue Árabe. Os animais foram distribuídos em cinco grupos, cada grupo foi composto por quatro cavalos. O grupo controle não recebeu óleo e os demais foram suplementados com 6, 12, 18 e 24% de óleo. Os animais foram submetidos a sete semanas consecutivas de exercício em esteira rolante e trilha. Analisou-...

  4. ENVENENAMENTO DE CÃES POR BUFADIENOLÍDEOS (Substâncias encontradas na secreção das glândulas paratóides dos sapos do gênero Bufo POISONING OF DOGS BY BUFADIENOLIDES (SUBSTANCES FOUND IN THE PARATOID GLAND SECRETION OF FROGS OF THE GENUS Bufo

    Directory of Open Access Journals (Sweden)

    Lourival Pereira Nunes

    2007-09-01

    Full Text Available

    Os autores estudaram o efeito da aplicação na mucosa bucal de cães, do produto secretado pelas glândulas paratóides de sapos do gênero Bufo. Os cães apresentaram sinais de graves intoxicações, imediatamente após a aplicação do referido produto.

    In the present paper, the authors report the effects of toad’s poison in dogs. This is the first case describe in Goiás.

  5. ORIGIN OF THE FACIAL ARTERY FROM THE LINGUAL-FACIAL TRUNK AND ITS COURSE THROUGH THE SUBMANDIBULAR SALIVARY GLAND: A CASE REPORT. Origen de la arteria facial desde el tronco lingual-facial y su curso a través de la glándula salival submandibular: informe

    Directory of Open Access Journals (Sweden)

    Srinivasa Rao Sirasanagandla

    2016-03-01

    Full Text Available La disección cuidadosa del tercio posterior de la parte superficial de la glándula salival submandibular es uno de los pasos quirúrgicos esenciales en la extirpación endoscópica glandular, evitando daños en la arteria facial. Un buen conocimiento de la poco común relación entre la arteria facial y la glándula salival submandibular es de vital importancia para llevar a cabo de forma eficiente y segura la extirpación de la glándula submandibular. Las variaciones del patrón de ramificación de la arteria facial son bien conocidas y han sido expuestas en el pasado. Sin embargo, las variaciones en su origen y trayectoria son poco frecuentes. Durante una rutinaria disección de cabeza y cuello para los estudiantes universitarios de Medicina, observamos la inusual trayectoria de la arteria facial en el triángulo digástrico derecho en un cadáver de un varón de origen indio de aproximadamente 60 años. La arteria facial derecha se originó de la común lingual-facial del tronco por encima del nivel del asta mayor del hueso hioides, y luego atravesar a través de la sustancia de la parte superficial de la glándula submandibular, sin la formación de un bucle. Después la arteria entraba en la cara por el ángulo anteroinferior del masetero. A continuación, en su trayectoria intraglandular, esta arteria mostraba pequeñas ramificaciones glandulares.  Careful dissection of the posterior one third of the superficial part of the submandibular salivary gland is one of the essential surgical steps in endoscopic glandular excision, to avoid injury to the facial artery. A sound knowledge of unusual relationship of the facial artery with the submandibular salivary gland is essentially important to perform the safe and efficient submandibular gland excision. Different types of variations in the branching pattern of the facial artery have been reported in the past. However, variations in the origin and course of the facial artery are very rare

  6. Evolución de la incidencia de los cánceres de glándulas salivales mayores en España (1978-2002 Trends in the incidence of major salivary gland cancer in Spain (1978-2002

    Directory of Open Access Journals (Sweden)

    Dyego Leandro Bezerra de Souza

    2012-02-01

    Full Text Available Objetivo: Analizar la evolución de las tasas de incidencia de los cánceres de glándulas salivales mayores en los Registros de Cáncer de Base Poblacional españoles. Métodos: Se seleccionaron los registros con un mínimo de 10 años de seguimiento: Albacete, Asturias, Granada, Murcia, Navarra, Tarragona y Zaragoza. Se calcularon las tasas de incidencia ajustadas a la población española a mitad del periodo. La tendencia de las tasas ajustadas se analizó con el programa joinpoint para 1991-2001. Resultados: El análisis joinpoint reveló una tendencia de reducción estadísticamente significativa, con un porcentaje anual de cambio del -5,3% (intervalo de confianza del 95%: -8,7 a -1,8. Conclusión: La incidencia de los cánceres de glándulas salivales mayores en España ha sido baja y se ha identificado una tendencia a la reducción entre 1991 y 2001.Objective: To analyze the trend in the incidence rates of major salivary gland cancer in population-based cancer registries in Spain. Methods: The following Spanish cancer registries with a minimum follow-up period of 10 years were selected: Albacete, Asturias, Granada, Murcia, Navarre, Tarragona and Zaragoza. Adjusted incidence rates were calculated. Joinpoint software was utilized to calculate change estimations in incidence rates for the period 1991-2001. Results: Joinpoint analysis revealed a statistically significant decreasing trend with an annual percent change of -5.3% (95% CI: -8.7 to -1.8. Conclusion: The incidence of major salivary gland cancers in Spain was low and a decreasing trend was identified between 1991 and 2001.

  7. Utilização de farinha de soja (Glycine max e de quinoa (Chenopodium quinoa no preparo de macarrão caseiro sem glúten
    Using of soy bean (Glycine max and quinoa (Chenopodium quinoa fl our to make homemade pasta without gluten

    Directory of Open Access Journals (Sweden)

    P. KIRINUS

    2011-04-01

    Full Text Available

    Sabendo da reduzida disponibilidade de produtos específi cos e indispensáveis para a melhoria da qualidade de vida de pacientes celíacos, este trabalho teve como objetivo elaborar dois tipos de massa para macarrão caseiro como alternativa alimentar para indivíduos que apresentam restrição ao consumo de glúten. Os macarrões foram elaborados por meio das seguintes etapas: homogeneização parcial, hidratação, amassamento e moldagem da massa, seccionamento em macarrão tipo talharim e cozimento em água e sal. Para realização da análise sensorial, participaram 40 provadores não treinados e não portadores de doença celíaca, os quais receberam três amostras de macarrão de farinha de trigo, macarrão de soja e macarrão de quinoa. A massa elaborada com farinha de soja foi mais bem aceita do que a elaborada com farinha de quinoa, podendo então ser sugerida como alternativa para a não privação de alimentos à base de glúten. Com o presente estudo, pôde-se concluir que existe a viabilidade de elaboração de macarrão caseiro do tipo talharim com farinha de soja ou de quinoa. Contudo, a massa elaborada com farinha de soja sugere características sensoriais mais promissoras.

  8. Dry eye evaluation and immunohistochemical study of the conjunctiva and lacrimal gland of patients submitted to bone marrow transplantation Alterações oculares e imunohistoquímicas na conjuntiva e glândula lacrimal de pacientes submetidos ao transplante de medula óssea

    Directory of Open Access Journals (Sweden)

    Rosana Nogueira Pires da Cunha

    2003-01-01

    /c subpopulation in the conjunctiva and in the LG of patients with ocular GVHD after the transplantation. CONCLUSIONS: Patients submitted to allogeneic BMT may develop ocular GHVD characterized by KCS. The immunohistochemical study of the conjunctiva and lacrimal gland biopsies of these patients suggested that these tissues are the target of the T cell mediated immunological reaction.OBJETIVOS: A glândula lacrimal e a conjuntiva são os tecidos oculares primariamente afetados pela doença enxerto-versus-hospedeiro (DEVH crônica, e seu envolvimento clínico é caracterizado por ceratoconjuntivite seca (CCS. Os objetivos deste estudo foram os de avaliar a freqüência dos sintomas e sinais de CCS em pacientes submetidos ao transplante de medula óssea (TMO e identificar a população celular por análise imuno-histoquímica de biópsias da conjuntiva e glândula lacrimal desses pacientes, correlacionando esses achados com o quadro clínico ocular. MÉTODOS: Quarenta e sete pacientes foram classificados em dois grupos: Grupo I, pacientes com DEVH ocular, submetidos ao TMO alogênico e Grupo II, pacientes sem DEVH ocular, submetidos ao TMO alogênico e autólogo. Exame ocular completo, incluindo testes clínicos da função lacrimal, e biópsias de conjuntiva e glândula lacrimal foram realizadas nos períodos pré e pós-transplante. Estudou-se a população celular, por meio de análise imuno-histoquímica das biópsias, utilizando um painel de anticorpos monoclonais. RESULTADOS: Dos 28 (82,4% pacientes com DEVH crônica, 13 (46,4% apresentaram DEVH ocular. Dos seis pacientes sem DEVH, um (16,7% apresentou DEVH ocular. Nenhum paciente submetido a TMO autólogo apresentou DEVH ocular e 14 (41,2% dos 34 pacientes com TMO alogênico desenvolveram DEVH ocular. Todos os pacientes com DEVH ocular (Grupo I apresentaram sintomas e os mais freqüentes foram ardor, sensação de corpo estranho, visão borrada e secura ocular. O teste de rosa bengala foi um dos mais sensíveis e o exame

  9. Human Monkeypox

    National Research Council Canada - National Science Library

    Wilson, Mary E; Hughes, James M; McCollum, Andrea M; Damon, Inger K

    2014-01-01

    Human monkeypox is found primarily in forested areas of Central Africa. This article provides a basic review of the clinical, epidemiological, and biological factors that contribute to human disease...

  10. Human microbiomics

    OpenAIRE

    Rajendhran, J.; Gunasekaran, P.

    2010-01-01

    The sequencing of the human genome has driven the study of human biology in a significant way and enabled the genome-wide study to elucidate the molecular basis of complex human diseases. Recently, the role of microbiota on human physiology and health has received much attention. The influence of gut microbiome (the collective genomes of the gut microbiota) in obesity has been demonstrated, which may pave the way for new prophylactic and therapeutic strategies such as bacteriotherapy. The sig...

  11. Human Development

    NARCIS (Netherlands)

    D.R. Gasper (Des)

    2009-01-01

    textabstract‘Human development’ language spread gradually in circles of national and international development policy and planning from the 1970s and acquired a definitive form in the 1990s in the United Nations Development Programme’s Human Development Reports (HDRs). Human development was defined

  12. Human Smuggling

    NARCIS (Netherlands)

    Siegel - Rozenblit, Dina|info:eu-repo/dai/nl/152524096; Zaitch, Damian|info:eu-repo/dai/nl/183348486

    2014-01-01

    Human smuggling is based on a consensus between smuggler, smuggled, and his/her family (which usually guarantees or effectuates payment). However, unauthorized immigrants are violating immigration laws and human smugglers are profiting from enabling illegal immigration. Both human smuggling and its

  13. Human Technology and Human Affects

    DEFF Research Database (Denmark)

    Fausing, Bent

    2009-01-01

    Human Technology and Human Affects  This year Samsung introduced a mobile phone with "Soul". It was made with a human touch and included itself a magical touch. Which function does technology and affects get in everyday aesthetics like this, its images and interactions included this presentation...... will ask and try to answer. The mobile phone and its devices are depicted as being able to make a unique human presence, interaction, and affect. The medium, the technology is a necessary helper to get towards this very special and lost humanity. Without the technology, no special humanity - soul...... - is the prophecy. This personification or anthropomorphism is important for the branding of new technology. The technology is seen as creating a technotranscendens towards a more qualified humanity, which is in contact with the fundamental human values like intuition, vision, and sensing; all the qualities...

  14. Human Parvoviruses.

    Science.gov (United States)

    Qiu, Jianming; Söderlund-Venermo, Maria; Young, Neal S

    2017-01-01

    Parvovirus B19 (B19V) and human bocavirus 1 (HBoV1), members of the large Parvoviridae family, are human pathogens responsible for a variety of diseases. For B19V in particular, host features determine disease manifestations. These viruses are prevalent worldwide and are culturable in vitro, and serological and molecular assays are available but require careful interpretation of results. Additional human parvoviruses, including HBoV2 to -4, human parvovirus 4 (PARV4), and human bufavirus (BuV) are also reviewed. The full spectrum of parvovirus disease in humans has yet to be established. Candidate recombinant B19V vaccines have been developed but may not be commercially feasible. We review relevant features of the molecular and cellular biology of these viruses, and the human immune response that they elicit, which have allowed a deep understanding of pathophysiology. Copyright © 2016 American Society for Microbiology.

  15. Human Rights/Human Needs.

    Science.gov (United States)

    Canning, Cynthia

    1978-01-01

    The faculty of Holy Names High School developed an interdisciplinary human rights program with school-wide activities focusing on three selected themes: the United Nations Universal Declaration of Human Rights, in conjunction with Human Rights Week; Food; and Women. This article outlines major program activities. (SJL)

  16. Insulin-like growth factor-binding protein-3 promotes transforming growth factor-β1-mediated epithelial-to-mesenchymal transition and motility in transformed human esophageal cells

    Science.gov (United States)

    Ohashi, Shinya; Wong, Gabrielle S.; Ahmadi, Azal; Kalman, Ross A.; Budo, Daniela; Klein-Szanto, Andres J.; Herlyn, Meenhard; Diehl, J. Alan; Nakagawa, Hiroshi

    2010-01-01

    Insulin-like growth factor-binding protein (IGFBP)-3 is overexpressed frequently in esophageal squamous cell carcinoma. Yet, the role of IGFBP3 in esophageal tumor biology remains to be elucidated. We find that IGFBP3 facilitates transforming growth factor (TGF)-β1-mediated epithelial-to-mesenchymal transition (EMT) in transformed human esophageal epithelial cells, EPC2–hTERT–EGFR–p53R175H. In organotypic 3D culture, a form of human tissue engineering, laser-capture microdissection revealed concurrent upregulation of TGF-β target genes, IGFBP3 and EMT-related genes in the cells invading into the stromal compartment. IGFBP3 enhanced TGF-β1-mediated EMT as well as transcription factors essential in EMT by allowing persistent SMAD2 and SMAD3 phosphorylation. TGF-β1-mediated EMT and cell invasion were enhanced by ectopically expressed IGFBP3 and suppressed by RNA interference directed against IGFBP3. The IGFBP3 knockdown effect was rescued by IGFBP3I56G/L80G/L81G, a mutant IGFBP3 lacking an insulin-like growth factor (IGF)-binding capacity. Thus, IGFBP3 can regulate TGF-β1-mediated EMT and cell invasion in an IGF or insulin-like growth factor 1 receptor-independent manner. IGFBP3I56G/L80G/L81G also promoted EMT in vivo in a Ras-transformed human esophageal cell line T-TeRas upon xenograft transplantation in nude mice. In aggregate, IGFBP3 may have a novel IGF-binding independent biological function in regulation of TGF-β1-mediated EMT and cell invasion. PMID:20513670

  17. Insulin-like growth factor-binding protein-3 promotes transforming growth factor-{beta}1-mediated epithelial-to-mesenchymal transition and motility in transformed human esophageal cells.

    Science.gov (United States)

    Natsuizaka, Mitsuteru; Ohashi, Shinya; Wong, Gabrielle S; Ahmadi, Azal; Kalman, Ross A; Budo, Daniela; Klein-Szanto, Andres J; Herlyn, Meenhard; Diehl, J Alan; Nakagawa, Hiroshi

    2010-08-01

    Insulin-like growth factor-binding protein (IGFBP)-3 is overexpressed frequently in esophageal squamous cell carcinoma. Yet, the role of IGFBP3 in esophageal tumor biology remains to be elucidated. We find that IGFBP3 facilitates transforming growth factor (TGF)-beta1-mediated epithelial-to-mesenchymal transition (EMT) in transformed human esophageal epithelial cells, EPC2-hTERT-EGFR-p53(R175H). In organotypic 3D culture, a form of human tissue engineering, laser-capture microdissection revealed concurrent upregulation of TGF-beta target genes, IGFBP3 and EMT-related genes in the cells invading into the stromal compartment. IGFBP3 enhanced TGF-beta1-mediated EMT as well as transcription factors essential in EMT by allowing persistent SMAD2 and SMAD3 phosphorylation. TGF-beta1-mediated EMT and cell invasion were enhanced by ectopically expressed IGFBP3 and suppressed by RNA interference directed against IGFBP3. The IGFBP3 knockdown effect was rescued by IGFBP3(I56G/L80G/L81G), a mutant IGFBP3 lacking an insulin-like growth factor (IGF)-binding capacity. Thus, IGFBP3 can regulate TGF-beta1-mediated EMT and cell invasion in an IGF or insulin-like growth factor 1 receptor-independent manner. IGFBP3(I56G/L80G/L81G) also promoted EMT in vivo in a Ras-transformed human esophageal cell line T-TeRas upon xenograft transplantation in nude mice. In aggregate, IGFBP3 may have a novel IGF-binding independent biological function in regulation of TGF-beta1-mediated EMT and cell invasion.

  18. Valor prognóstico do Ki-67 no carcinoma indiferenciado de grandes células de glândula salivar maior: estudo de 11 casos Prognostic significance of Ki-67 in great cell undifferentiated carcinoma of the major salivary glands: study of 11 cases

    Directory of Open Access Journals (Sweden)

    Túlio V. Barbosa

    2003-10-01

    Full Text Available INTRODUÇÃO: Estabelecer a relação entre o comportamento biológico e o prognóstico nas neoplasias indiferenciadas das glândulas salivares maiores. OBJETIVO: Identificação de parâmetros clínicos, histopatológicos, imunohistoquímicos e de proliferação celular (Ki-67 no prognóstico destas neoplasias. FORMA DE ESTUDO: Análise de espécimes através de procedimentos histológicos e imunohistoquímicos e sua relação com o prognóstico destas neoplasias. MATERIAL E MÉTODO: Onze casos de carcinoma indiferenciado tipo grandes células das glândulas salivares maiores, diagnosticados e tratados no Departamento de Cirurgia de Cabeça e Pescoço e Otorrinolaringologia do Hospital Heliópolis, Hosphel, São Paulo, no período de 1977 a 2000. Foram revistos e subclassicados por perfis de positividade para citoqueratinas de alto e/ou baixos pesos moleculares. RESULTADOS: Sinalizou-se com um padrão imunohistoquímico de diferenciação histogênica bidirecional (tipo mucoepidermóide, e unidirecionais do tipo epidermóide ou ductal/glandular, visando sobretudo identificar a influência de parâmetros demográficos, clínicos, anátomo-patológicos (índice mitótico, e do índice de imunoproliferação celular (ki-67 clone MIB-1. Os resultados mostraram significativa ocorrência em indivíduos na 5ª década de vida, brancos, na glândula parótida, ao mesmo tempo que não demostrou diferença estatística em relação ao critério gênero. O índice mitótico e o índice de imunoproliferação celular (Ki-67, apresentaram-se estatisticamente significativos (pINTRODUCTION: To establish the relation between the biologic behaviour and the prognosis of the undifferentatiated carcinoma of major salivary glands. OBJECTIVE: Identification of clinical histological, imunohistochemical parameters and its influence on the prognosis. STUDY DESIGN: Specimens analysis through histological and imunoihistochemical procedures. MATERIAL AND METHOD: Eleven

  19. Importancia de la reconstrucción volumétrica y del pliegue glúteo en los parapléjicos con úlceras isquiáticas Enis Sarmiento IV Importance of volume and gluteal fold reconstruction in paraplegic patients with ischial ulcers type Enis Sarmiento IV

    Directory of Open Access Journals (Sweden)

    E. Revelo Jirón

    2011-06-01

    Full Text Available Los parapléjicos rehabilitados son propensos a sufrir úlceras isquiáticas como complicación más frecuente. Cuando estas úlceras tienen compromiso óseo, su tratamiento solo puede ser quirúrgico. Bajo estas condiciones los colgajos miocutáneos locales son parte de la solución. En el artículo presentamos una serie personal de 10 pacientes parapléjicos rehabilitados con úlceras isquiáticas reconstruidas utilizando un colgajo miocutáneo en isla de la porción inferior del glúteo mayor transferido a través de un túnel subcutáneo. Ninguno de los pacientes de nuestro grupo de estudio sufrió recidiva y todos han tenido una buena evolución a largo plazo. La aportación principal del presente trabajo es hacer hincapié en respetar en estos casos 3 principios utilizados en Cirugía Estética: las incisiones quirúrgicas deben efectuarse en los pliegues naturales para evitar secuelas estético-funcionales; debemos dejar mínimas cicatrices y obtener una restauración volumétrica corporal. En ese sentido pensamos que el diseño de los colgajos debe respetar rigurosamente la orientación del pliegue glúteo y aportar un buen almohadillado para reconstruir el capital volumétrico de la zona glútea; además es primordial dejar pocas cicatrices para no aumentar los riesgos locales debido a la falta de trofismo de la piel. De esta manera, creemos que se evitan las recidivas y las complicaciones.Paraplegic patients, during their rehabilitation period, usually develop ischial ulcers as the most common complication. When there is bone involvement only the surgical approach can be successful. Myocutaneous flaps are part of this approach. We present a sample of 10 paraplegic patients under rehabilitation suffering ischial ulcers that were handled with myocutaneous island flaps obtained from the lower bundles of gluteus maximus and transferred though a subcutaneous tunnel. All these patients have had a long term good evolution with no recurrences

  20. Digital Humanities

    DEFF Research Database (Denmark)

    Brügger, Niels

    2016-01-01

    Digital humanities is an umbrella term for theories, methodologies, and practices related to humanities scholarship that use the digital computer as an integrated and essential part of its research and teaching activities. The computer can be used for establishing, finding, collecting......, and preserving material to study, as an object of study in its own right, as an analytical tool, or for collaborating, and for disseminating results. The term "digital humanities" was coined around 2001, and gained currency within academia in the following years. However, computers had been used within...... the humanities for decades, starting with research fields such as humanities computing or computational linguistics in the 1950s, and later new media studies and internet studies. The historical development of digital humanities has been characterized by a focus on three successive, but co-existing types...

  1. Oxygenation by a superhydrophobic slip G/L contactor

    NARCIS (Netherlands)

    Karatay, Elif; Lammertink, Rob G.H.

    2012-01-01

    The compelling need for an efficient supply of gases into liquids or degassing of fluids within confined microchannels triggered our study on membrane assisted microchemical systems. Porous hydrophobic flat/micro-structured polyvinylidene fluoride (PVDF) membranes were fabricated and integrated in a

  2. Terca opacidad del glásnost mexicano

    OpenAIRE

    Francisco Prieto

    2015-01-01

    Los cambios del papel estatal en el manejo de la economía mexicana son de incuestionable trascendencia. Pero en relación a la reforma política y la autonomía informativa de los medios, las medidas son mucho más modestas. El autor recuerda los antecedentes del peculiar sistema político mexicano y su relación con los medios de comunicación. También señala los factores que propician los cambios en curso y es optimista respecto al ritmo de la modernización de los medios en México.

  3. Glædelig jul, du er fallit

    DEFF Research Database (Denmark)

    Sodemann, Morten

    2014-01-01

    En 37 årig mand, flygtning med fast opholdstilladelse i Danmark, kommer alvorligt fysisk til skade på sin arbejdsplads, sygemeldes og mister det danske sprog. Krigsoplevelserne, der ellers var kommet på afstand, kommer tilbage som mareridt og genoplevelser, der invaliderer hans arbejdsevne yderli...

  4. Terca opacidad del glásnost mexicano

    Directory of Open Access Journals (Sweden)

    Francisco Prieto

    2015-01-01

    Full Text Available Los cambios del papel estatal en el manejo de la economía mexicana son de incuestionable trascendencia. Pero en relación a la reforma política y la autonomía informativa de los medios, las medidas son mucho más modestas. El autor recuerda los antecedentes del peculiar sistema político mexicano y su relación con los medios de comunicación. También señala los factores que propician los cambios en curso y es optimista respecto al ritmo de la modernización de los medios en México.

  5. Von glücklichen Dänen lernen?

    DEFF Research Database (Denmark)

    Stöber, Birgit

    Die Kopenhagener Kulturgeografin Birgit Stöber führt in die dänische Debatte ein, berichtet von ungewöhnlichen dänischen Lösungsmodellen, wie dem Abrissfonds für verfallene Dorfhäuser, dem städtebaulichen Engagement der philanthropischen Vereinigung Realdania oder vom überaus populären politische...

  6. FROG: The Fast And Realistic OpenGL Event Displayer

    CERN Document Server

    Quertenmont, Loic

    2009-01-01

    FROG [1] [2] is a generic framework dedicated to visualisation of events in high energy experiment. It is suitable to any particular physics experiment or detector design. The code is light (< 3 MB) and fast (browsing time 20 events per second for a large High Energy Physics experiment) and can run on various operating systems, as its object-oriented structure (C++) relies on the cross-platform OPENGL [3] and GLUT [4] libraries. Moreover, FROG does not require installation of third party libraries for the visualisation. This documents describes the features and principles of FROG version 1.106, its working scheme and numerous functionalities such as: 3D and 2D visualisation, graphical user interface, mouse interface, configuration files, production of pictures of various format, integration of personal objects, etc. Finally the application of FROG for physic experiment/environement, such as Gastof, CMS, ILD, Delphes will be presented for illustration.

  7. Effects of platinic chloride on Tetrahymena pyrifromis GL

    DEFF Research Database (Denmark)

    Nilsson, Jytte R.

    1992-01-01

    Cellebiologi, platinum(IV)chloride, endocytosis, detoxification, cell proliferation, fine structure, cisplatin......Cellebiologi, platinum(IV)chloride, endocytosis, detoxification, cell proliferation, fine structure, cisplatin...

  8. Human evolution

    DEFF Research Database (Denmark)

    Llamas, Bastien; Willerslev, Eske; Orlando, Ludovic Antoine Alexandre

    2017-01-01

    The field of human ancient DNA (aDNA) has moved from mitochondrial sequencing that suffered from contamination and provided limited biological insights, to become a fully genomic discipline that is changing our conception of human history. Recent successes include the sequencing of extinct hominins......, and true population genomic studies of Bronze Age populations. Among the emerging areas of aDNA research, the analysis of past epigenomes is set to provide more new insights into human adaptation and disease susceptibility through time. Starting as a mere curiosity, ancient human genetics has become...

  9. Hidradenite supurativa crônica perianal e glútea: tratamento cirúrgico com ressecção ampla e rotação de retalho dermogorduroso Perianal and gluteal hidradenitis suppurative chronic: surgical treatment with a great resection and rotation of skin flaps

    Directory of Open Access Journals (Sweden)

    Pedro Roberto de Paula

    2010-09-01

    Full Text Available OBJETIVO: Avaliar a utilização de retalho dermogorduroso de vizinhança, numa única etapa, na reparação da área resultante da ressecção de lesões de hidradenite supurativa. PACIENTE E MÉTODOS: Estudo epidemiológico transversal e retrospectivo de prontuários de pacientes com hidradenite supurativa crônica extensa em regiões perianal e glútea submetidos à ressecção ampla e rotação de retalho cutâneo de vizinhança, no período de janeiro de 2000 a novembro de 2008. RESULTADOS: O retalho dermogorduroso permitiu, em única etapa, a cobertura total da área ressecada em oito pacientes. Não houve necrose ou infecção em nenhum dos casos. No seguimento, seis pacientes compareceram a todas as consultas ambulatoriais agendadas. Todos se mostraram satisfeitos com o resultado. CONCLUSÃO: O tratamento da hidroadenite supurtiva perianal e/ou glútea deve ser individualizado segundo a extensão, gravidade e grau de interferência na qualidade de vida. A ressecção cirúrgica com procedimentos plásticos como a rotação de retalho de vizinhança em única etapa é segura e traz resultados satisfatórios e deve ser sempre considerada nos casos crônicos, extensos e refratários ao tratamento clínico e com grandes áreas ressecadas.OBJECTIVE: To evaluate the use of skin flaps neighborhood in a single step in the repair of the area from the resection of lesions of Suppurative Hidradenitis. METHODS: Retrospective cross-sectional epidemiological study of patients with chronic extensive Suppurative Hidradenitis in perianal and gluteal regions underwent extensive resection and skin flap rotation of the neighborhood, from January 2000 to November 2008. RESULTS: The skin flaps allowed in single step, the total coverage of the area resected in eight patients. There was no necrosis or infection in any cases. Following, six patients attended all scheduled outpatient consultations. All were satisfied with the result. CONCLUSIONS: The treatment of

  10. Human Rights, Human Needs, Human Development, Human Security - Relationships between four international human discourses.

    NARCIS (Netherlands)

    D.R. Gasper (Des)

    2007-01-01

    markdownabstractAbstract: Human rights, human development and human security form increasingly important, partly interconnected, partly competitive and misunderstood ethical and policy discourses. Each tries to humanize a pre-existing and unavoidable major discourse of everyday life, policy and

  11. In Vivo Imaging of Human Malignant Mesothelioma Grown Orthotopically in the Peritoneal Cavity of Nude Mice

    Directory of Open Access Journals (Sweden)

    Mingqian Feng, Jingli Zhang, Miriam Anver, Raffit Hassan, Mitchell Ho

    2011-01-01

    Full Text Available Malignant mesothelioma (MM causes significant morbidity and mortality in patients. With increasing efforts devoted to developing therapeutics targeting mesothelioma, a xenograft mouse model with in vivo tumor imaging is especially desired for evaluating anti-tumor therapies. In the present study, we fluorescently labeled the NCI-H226 human mesothelioma cell line by a lentiviral vector harboring a luciferase-GFP (Luc/GFP fusion gene driven by the RNA polymerase II promoter. After single-cell cloning by flow cytometry, a clone (named LMB-H226-GL that stably expresses high levels of Luc/GFP was obtained. The in vivo tumorigenicity of Luc/GFP-labeled LMB-H226-GL was determined by using intraperitoneal injections of the cells in nude mice. LMB-H226-GL was found to be able to consistently form solid tumors in the peritoneum of mice. Tumor growth and aggressive progression could be quantitated via in vivo bioluminescence imaging. The model exhibited the pathological hallmarks consistent with the clinical progression of MM in terms of tumor growth and spread inside the peritoneal cavity. To evaluate the in vivo efficacy of drugs targeting mesothelioma, we treated mice with SS1P, a recombinant immunotoxin currently evaluated in Phase II clinical trials for treatment of mesothelioma. All the tumor-bearing mice had a significant response to SS1P treatment. Our results showed that this is a well-suited model for mesothelioma, and may be useful for evaluating other novel agents for mesothelioma treatment in vivo.

  12. Human Rights and Human Nature

    Directory of Open Access Journals (Sweden)

    Vittorio Possenti

    2013-11-01

    Full Text Available There seems to be two different versions of human rights in Western tradition: say Rationalistic and Christian; the former adopted in revolutionary France, the latter highly developed in Renaissance Spain. Current relativistic criticisms attempt to deny the universality of human rights alleging that this theory has been created in Western countries or it has no strong justification, and therefore cannot have universal approach; but this objection can be dismissed with an alternative justification of human rights.

  13. Think Human

    DEFF Research Database (Denmark)

    Nielsen, Charlotte Marie Bisgaard

    2013-01-01

    years' campaigns suggests that the theory of communication underlying the campaign has its basis in mechanical action rather than in human communication. The practice of 'Communication design' is investigated in relation to this metaphorical 'machine thinking' model of communication and contrasted...... with the human-centered theory of communication advocated by integrationism....

  14. Human trichuriasis

    DEFF Research Database (Denmark)

    Betson, Martha; Søe, Martin Jensen; Nejsum, Peter

    2015-01-01

    Human trichuriasis is a neglected tropical disease which affects hundreds of millions of people worldwide and is particularly prevalent among children living in areas where sanitation is poor. This review examines the current knowledge on the taxonomy, genetics and phylogeography of human Trichuris...

  15. Human kapital

    DEFF Research Database (Denmark)

    Grosen, Anders; Nielsen, Peder Harbjerg

    2007-01-01

    finansiel og human kapital. Den traditionelle rådgivnings snævre synsvinkel kan føre til forkerte investeringsråd. Der skal derfor opfordres til, at de finansielle virksomheder i tilrettelæggelsen af deres rådgivning af private kunder systematisk inddrager den humane kapitals størrelse og karakteristika i...

  16. Histologia e morfometria das glândulas das junções infundíbulo-magno e útero-vagina de codorna doméstica Histology and morphometry of the infundibulum-magnum and uterus-vaginal junctions of spotted quails

    Directory of Open Access Journals (Sweden)

    Carime Moraes

    2009-04-01

    Full Text Available A análise comparativa das glândulas armazenadoras de espermatozóides das junções infundíbulo-magno e útero-vagina do oviduto da codorna Nothura maculosa foi realizada durante a fase reprodutiva. As aves foram eutanasiadas por inalação com éter etílico, após a pesagem. Após a laparotomia e o deslocamento cranial do esterno, foram coletadas amostras de dois centímetros da junção infundíbulo-magno e útero-vagina. Os fragmentos foram fixados em solução de Bouin por 24 horas e, posteriormente, banhos sucessivos de álcool 70% foram aplicados sobre as amostras. Em seguida, as amostras foram desidratadas em uma série de concentração crescente de alcoóis (80, 90, 95 e 3x100%, diafanizadas em xilol e incluídas em parafina. Cortes histológicos de sete micrômetros de espessura foram obtidos e corados pela técnica da Hematoxilina-eosina (HE, que foram analisados e fotomicrografados em um fotomicroscópio Olympus BX-50. Para a análise morfométrica, foram capturadas imagens das glândulas armazenadoras de espermatozóides da junção infundíbulo-magno e útero-vagina mediante o programa computacional "Image Pro Plus 4.1" da Cibernetics do Brasil, tornando-se possível a quantificação das mesmas. A mucosa da junção infundíbulo-magno apresentou pregas estreitas no infundíbulo e largas no magno, enquanto na junção útero-vagina mostrou-se amplamente pregueada na vagina e mais lisa na porção uterina. O número médio de glândulas armazenadoras de espermatozóides na junção infundíbulo-magno foi maior (11,7 glândulas (PThe comparative analysis of sperm-host glands of infundibulum-magnum and uterus-vagina junctions of Nothura maculosa quail oviduct was done during the reproductive phase. The birds were euthanized with ethyl ether after weight data were taken. After abdominal laparotomy and cranial displacement of the breastbone, two centimeters samples of infundibulum-magnum and uterus-vagina junctions were collected

  17. O modo de coaptação glótica em crianças no diagnóstico diferencial de alteração estrutural mínima The glottal closure in diagnostic of minor structural alterations

    Directory of Open Access Journals (Sweden)

    Noemi De Biase

    2004-08-01

    Full Text Available A configuração glótica varia durante a fonação, mesmo em indivíduos sem queixas vocais e sem alterações ao exame, de acordo com a idade, o sexo, o registro vocal, a freqüência fundamental, a tensão e as lesões. Observa-se junção completa ou incompleta da borda livre da prega vocal; quando incompleta, formam-se fendas de formatos variados. OBJETIVO: Nosso objetivo é encontrar no modo de coaptação glótica, durante a fonação sustentada da vogal /épsilon/ em crianças com alteração estrutural mínima, elementos que permitam diferenciá-lo dos indivíduos com nódulo vocal ou de indivíduos sem queixas vocais. MATERIAL E MÉTODO: Estudo retrospectivo de dados de crianças atendidas no período de 1996 a 2001. A amostra constou de imagens de laringes de crianças que apresentaram diagnóstico de alteração estrutural mínima, nódulo vocal e também de crianças sem queixas vocais, estes denominados normais. Destas imagens foi analisada a configuração glótica durante a fonação da vogal /épsilon/ e realizada análise estatística para a comparação entre os três grupos. RESULTADOS: As fendas triangulares são encontradas nos três grupos, enquanto que a fenda fusiforme só ocorreu nas alterações estruturais mínimas. CONCLUSÕES: A utilização do modo de coaptação glótica em crianças, como critério diagnóstico para diferenciar alteração estrutural mínima de nódulo vocal e de laringe normal, é relevante quando se observa fenda fusiforme, condição encontrada somente nas alterações estruturais mínimas; as fendas triangulares não se mostraram significantes para diferenciar alteração estrutural mínima de nódulo vocal e laringe normal.The glottal closure varies during phonation, even in subjects who bears no vocal complaints and no alterations on medical examination, according to age, sex, vocal register, fundamental frequency, tension and lesions. There has been noticed complete or incomplete junction

  18. [Cerebral infarction in human immunodeficiency virus infection].

    Science.gov (United States)

    Blanche, P; Toulon, P; de La Blanchardière, A; Sicard, D

    1995-06-03

    Patients infected with the human immunodeficiency virus (HIV) appear to have a high risk of ischaemic cerebral events. We observed two cases of cerebral infarction in patients with acquired immune deficiency syndrome (AIDS). In the first case, a 38-year-old homosexual with no cardiovascular risk other than smoking presented with rapidly progressive hemiparesia. Brain CT-scan visualized two infarcts in the territory of the right sylvian artery and the arteriography an occlusion of the internal carotid artery. In the second, a 37-year-old homosexual, hospitalization was required for a left-sided pure sensitive epilepsy seizure. There was no cardiovascular risk other than smoking. Magnetic resonance imaging showed parietal ischaemia and thrombus in the left atrium without atrial hypertrophy was seen at transoesophageal echocardiography. In both cases, there was no evidence of endocarditis, dissection of the neck vessels or disseminated intravascular coagulation nor of associated viral or bacterial infectious complication of AIDS. Angiographic findings eliminated cerebral vascularitis. Among the perturbed haemostasis factors previously reported in HIV+ patients, we observed free proteins S deficiency (68 and 43%) and heparin cofactor II deficiency (54 and 40%). Serum albumin was 33 and 32 g/l respectively. Outcome was favourable in both cases with anticoagulant therapy. These coagulation anomalies would not appear sufficient to explain cerebral infarction. Other mechanisms including immune complexed deposition, direct HIV toxicity for endothelial cells or the effect of cytokines on smooth muscles fibres and fibroblasts are probably more important causal factors.

  19. Human Computation

    CERN Multimedia

    CERN. Geneva

    2008-01-01

    What if people could play computer games and accomplish work without even realizing it? What if billions of people collaborated to solve important problems for humanity or generate training data for computers? My work aims at a general paradigm for doing exactly that: utilizing human processing power to solve computational problems in a distributed manner. In particular, I focus on harnessing human time and energy for addressing problems that computers cannot yet solve. Although computers have advanced dramatically in many respects over the last 50 years, they still do not possess the basic conceptual intelligence or perceptual capabilities...

  20. Human phantom

    CERN Multimedia

    CERN PhotoLab

    1973-01-01

    This human phantom has been received by CERN on loan from the State Committee of the USSR for the Utilization of Atomic Energy. It is used by the Health Physics Group to study personel radiation doses near the accelerators.

  1. Human expunction

    Science.gov (United States)

    Klee, Robert

    2017-10-01

    Thomas Nagel in `The Absurd' (Nagel 1971) mentions the future expunction of the human species as a `metaphor' for our ability to see our lives from the outside, which he claims is one source of our sense of life's absurdity. I argue that the future expunction (not to be confused with extinction) of everything human - indeed of everything biological in a terran sense - is not a mere metaphor but a physical certainty under the laws of nature. The causal processes by which human expunction will take place are presented in some empirical detail, so that philosophers cannot dismiss it as merely speculative. I also argue that appeals to anthropic principles or to forms of mystical cosmology are of no plausible avail in the face of human expunction under the laws of physics.

  2. Human brucellosis

    NARCIS (Netherlands)

    Franco, María Pía; Mulder, Maximilian; Gilman, Robert H.; Smits, Henk L.

    2007-01-01

    Human brucellosis still presents scientists and clinicians with several challenges, such as the understanding of pathogenic mechanisms of Brucella spp, the identification of markers for disease severity, progression, and treatment response, and the development of improved treatment regimens.

  3. Human Toxicity

    DEFF Research Database (Denmark)

    Jolliet, Olivier; Fantke, Peter

    2015-01-01

    This chapter reviews the human toxicological impacts of chemicals and how to assess these impacts in life cycle impact assessment (LCIA), in order to identify key processes and pollutants. The complete cause-effect pathway – from emissions of toxic substances up to damages on human health...... on characterisation factors means that results should by default be reported and interpreted in log scales when comparing scenarios or substance contribution! We conclude by outlining future trends in human toxicity modelling for LCIA, with promising developments for (a) better estimates of degradation halflives, (b......) the inclusion of ionization of chemicals in human exposure including bioaccumulation, (c) metal speciation, (d) spatialised models to differentiate the variability associated with spatialisation from the uncertainty, and (e) the assessment of chemical exposure via consumer products and occupational settings...

  4. [Three dimensional bioprinting technology of human dental pulp cells mixtures].

    Science.gov (United States)

    Xue, Shi-hua; Lv, Pei-jun; Wang, Yong; Zhao, Yu; Zhang, Ting

    2013-02-18

    To explore the three dimensional(3D)bioprinting technology, using human dental pulp cells (hDPCs) mixture as bioink and to lay initial foundations for the application of the 3D bioprinting technology in tooth regeneration. Imageware 11.0 computer software was used to aid the design of the 3D biological printing blueprint. Sodium alginate-gelatin hydrosol was prepared and mixed with in vitro isolated hDPCs. The mixture contained 20 g/L sodium alginate and 80 g/L gelatin with cell density of 1×10(6)/mL. The bioprinting of hDPCs mixture was carried out according to certain parameters; the 3D constructs obtained by printing were examined; the viability of hDPCs after printing by staining the constructs with calcein-AM and propidium iodide dye and scanning of laser scanning confocal microscope was evaluated. The in vitro constructs obtained by the bioprinting were cultured, and the proliferation of hDPCs in the constructs detected. By using Imageware 11.0 software, the 3D constructs with the grid structure composed of the accumulation of staggered cylindrical microfilament layers were obtained. According to certain parameters, the hDPCs-sodium alginate-gelatin blends were printed by the 3D bioprinting technology. The self-defined shape and dimension of 3D constructs with the cell survival rate of 87%± 2% were constructed. The hDPCs could proliferate in 3D constructs after printing. In this study, the 3D bioprinting of hDPCs mixtures was realized, thus laying initial foundations for the application of the 3D bioprinting technology in tooth regeneration.

  5. Human ehrlichiosis

    Directory of Open Access Journals (Sweden)

    Đokić Milomir

    2006-01-01

    Full Text Available Background. Human ehrlichiosis is a newly recognized disease. It is a tick-borne disease caused by several bacterial species of the genhus Erlichia. These are small gram-negative pleomorphic cocci, that are obligatory intracellular bacteria. Tick Ixodes is the principle vector in Europe, and Amblyomma americanum in the United States. Bacterial organisms replicate in a tick, and are transmited from infected cells in a vector to the blood cells of animals or humans. Human ehrlichiosis is a name for a group of diseases caused by different species of Ehrlichia. One of them is the disease named human monocytic ehrlichiosis, caused by Ehrlichia chaffeensis, and the other is a human granulocytic ehrlichiosis caused by Anaplasma phagocytophilia. Case report. We reported a 23-year-old patient admitted for the clinical treatment with the symptoms of high febrility (above 40 °C, headache, vomiting, general weakness and exhaustion, but without data on a tick bite. The patient was treated with trimetoprim-sulfamethoxazole for a week when Ehrlichia chaffeensis was confirmed by the immunofluoroscence test, and the therapy contimed with doxacyclin. Conclusion. Human ehrlichiosis is also present in our country, so this disease should be considered everyday, especially in infectology practice.

  6. High Expression of Human Cathepsin S by Recombinant Pichia pastoris with Cod Skin as an Organic Co-Nitrogen Source.

    Science.gov (United States)

    Li, Guiying Y; Fu, Man; Qin, Mei; Xue, Liming M

    2018-02-06

    Human cathepsin S production by recombinant Pichia pastoris using cod skin as the co-nitrogen source was investigated in this study. The addition of carbon sources of glycerol in the fed-batch phase and of methanol in the induction stage was also investigated. A new approach to the highly expression of human cathepsin S was developed using 90 g/L of cod skin (wet weight). After 24 h of the initial fermentation, 4% glycerol (v/v, glycerol/culture) was added once to enhance the cell density (OD600) in the cultivation. Then, adding and maintaining methanol at 0.5% (v/v, methanol/cultivation) after about 48 h of fermentation achieved a high expression of human cathepsin S in a 5-L bioreactor. The results demonstrate that the maximum activity of human cathepsin S in the fermentation supernatant reached 7,152 U/L after 96 h of methanol induction. The methylotrophic yeast P. pastoris grown in the medium containing cod skin (90 g/L) as the co-nitrogen source provided a 21% higher cell density (OD600) and 18.3% higher human cathepsin S yield than P. pastoris grown in BMGY medium. For the first time, human cathepsin S was successfully expressed by P. pastoris with cod skin as the co-nitrogen source. The glycerol fed-batch controlling strategy and method of maintaining methanol at a constant concentration of 0.5% (v/v, methanol/cultivation) in the induction stage was efficient for P. pastoris growth and the expression of human cathepsin S. © 2018 S. Karger AG, Basel.

  7. Human serum albumin-based nanoparticle-mediated in vitro gene delivery.

    Directory of Open Access Journals (Sweden)

    Monica Langiu

    Full Text Available The genetic treatment of neurodegenerative diseases still remains a challenging task since many approaches fail to deliver the therapeutic material in relevant concentrations into the brain. As viral vectors comprise the risk of immune and inflammatory responses, human serum albumin (HSA nanoparticles were found to represent a safer and more convenient alternative. Their ability to cross the blood-brain barrier (BBB and deliver drugs into the brain in order to enhance gene-based therapy has been previously demonstrated. The present study deals with the development of pGL3-PEI-coated HSA nanoparticles and subsequent in vitro testing in cerebellar granular and HeLa cells. The luciferase control vector pGL3 was chosen as reporter plasmid encoding for the firefly luciferase protein, linear polyethylenimine (22 kDa as endosomolytic agent for enhancing the cells' transfection. Studies on particle characteristics, their cellular uptake into aforementioned cell lines and on subcellular localisation, and transfection efficiency in the cerebellar cells proved the feasibility of nanoparticle-based gene delivery.

  8. Associations between Maternal Body Composition and Appetite Hormones and Macronutrients in Human Milk.

    Science.gov (United States)

    Kugananthan, Sambavi; Gridneva, Zoya; Lai, Ching T; Hepworth, Anna R; Mark, Peter J; Kakulas, Foteini; Geddes, Donna T

    2017-03-09

    Human milk (HM) appetite hormones and macronutrients may mediate satiety in breastfed infants. This study investigated associations between maternal adiposity and concentrations of HM leptin, adiponectin, protein and lactose, and whether these concentrations and the relationship between body mass index and percentage fat mass (%FM) in a breastfeeding population change over the first year of lactation. Lactating women (n = 59) provided milk samples (n = 283) at the 2nd, 5th, 9th and/or 12th month of lactation. Concentrations of leptin, adiponectin, total protein and lactose were measured. Maternal %FM was measured using bioimpedance spectroscopy. Higher maternal %FM was associated with higher leptin concentrations in both whole (0.006 ± 0.002 ng/mL, p = 0.008) and skim HM (0.005 ± 0.002 ng/mL, p = 0.007), and protein (0.16 ± 0.07 g/L, p = 0.028) concentrations. Adiponectin and lactose concentrations were not associated with %FM (0.01 ± 0.06 ng/mL, p = 0.81; 0.08 ± 0.11 g/L, p = 0.48, respectively). Whole milk concentrations of adiponectin and leptin did not differ significantly over the first year of lactation. These findings suggest that the level of maternal adiposity during lactation may influence the early appetite programming of breastfed infants by modulating concentrations of HM components.

  9. Modes of rouleaux formation of human red blood cells in polyvinylpyrrolidone and dextran solutions.

    Science.gov (United States)

    Sewchand, L S; Canham, P B

    1979-11-01

    The mechanics by which normal human erythrocytes join on a plastic cover slip into two cell doublets and larger aggregates of rouleaux were studied microscopically. Polyvinylpyrrolidone (PVP-360) or dextran (DX-70 or DX-110) were used as the rouleau agents. The minimum concentration of the rouleau-inducing agents required to form doublets was 1 g/L for PVP-360 and 5 g/L for the DXs. Three modes of interaction were observed in Ringer's solution with PVP or DX, cresting and flipping (involving no intercellular sliding) and a sliding mode of doublet formation (involving less gravitational work and less cellular deformation). The sliding mechanism occurred in suspensions with the lower concentrations of the rouleau agent but was also observed when geometric constraints prevented the nonsliding interaction of larger groups of cells in the higher concentrations of the rouleau agent. The technique provides a sensitive index for studying the combined effect of cellular flexibility and intercellular adhesion. Significant changes were observed for reduced membrane surface charge or reduced ionic calcium.

  10. Associations between Maternal Body Composition and Appetite Hormones and Macronutrients in Human Milk

    Directory of Open Access Journals (Sweden)

    Sambavi Kugananthan

    2017-03-01

    Full Text Available Human milk (HM appetite hormones and macronutrients may mediate satiety in breastfed infants. This study investigated associations between maternal adiposity and concentrations of HM leptin, adiponectin, protein and lactose, and whether these concentrations and the relationship between body mass index and percentage fat mass (%FM in a breastfeeding population change over the first year of lactation. Lactating women (n = 59 provided milk samples (n = 283 at the 2nd, 5th, 9th and/or 12th month of lactation. Concentrations of leptin, adiponectin, total protein and lactose were measured. Maternal %FM was measured using bioimpedance spectroscopy. Higher maternal %FM was associated with higher leptin concentrations in both whole (0.006 ± 0.002 ng/mL, p = 0.008 and skim HM (0.005 ± 0.002 ng/mL, p = 0.007, and protein (0.16 ± 0.07 g/L, p = 0.028 concentrations. Adiponectin and lactose concentrations were not associated with %FM (0.01 ± 0.06 ng/mL, p = 0.81; 0.08 ± 0.11 g/L, p = 0.48, respectively. Whole milk concentrations of adiponectin and leptin did not differ significantly over the first year of lactation. These findings suggest that the level of maternal adiposity during lactation may influence the early appetite programming of breastfed infants by modulating concentrations of HM components.

  11. Cryptosporidium Infection of Human Intestinal Epithelial Cells Increases Expression of Osteoprotegerin: A Novel Mechanism for Evasion of Host Defenses

    Science.gov (United States)

    2008-02-20

    L D-glucose, 0.58 g/L L-glutamine, 3.7 g/L sodium bicarbonate , 0.2 g/L bovine bile, 0.004 g/L folic acid , 0.001 g/L 4-aminobenzoic acid , 0.004 g/L D...calcium pantothenate, 0.88 g/L ascorbic acid , 1% heat-inactivated fetal bovine serum [FBS], 2.4 g/L HEPES, 100,000 U/L penicillin, 100,000 g/L...washed with 0.15 mol/L PBS (pH 7.2), centrifuged (3000 g, 15 min, 4°C), and treated with acidic H2O (pH 2.5) for 20 min at 37°C, with vortexing every 5 min

  12. Mouth floor enlargements related to the sublingual glands in edentulous or partially edentulous patients: a microscopic study Tumefações do soalho bucal relacionadas às glândulas sublinguais em pacientes edêntulos ou parcialmente edêntulos: estudo microscópico

    Directory of Open Access Journals (Sweden)

    Liogi Iwaki Filho

    2006-08-01

    Full Text Available Mouth floor enlargements (MFE are observed in edentulous and partially edentulous patients, impairing denture fitting, and have recently been described in the literature as hyperplasias of the sublingual glands. OBJECTIVE: This study aims at describing the microscopic aspects of MFE that contribute to their final diagnosis. METHODS: Twenty-four specimens were surgically removed from the enlarged mouth floor of 19 patients (15 females and 4 males. Patient age ranged from 48 to 74 years, with a mean of 57 years. The main surgical indication was to permit or improve the fitting of dentures. Six patients were completely edentulous and 13 were partially edentulous. The material was processed for microscopic examination and stained with hematoxylin-eosin, Mallory's trichrome and periodic-acid Schiff (PAS. RESULTS AND CONCLUSIONS: The epithelium of the mouth floor was normal in 17 cases, hyperplastic in 4 and atrophic in 3. Six of the 24 sublingual glands removed were microscopically normal, while the other specimens presented acinar atrophy with hyperplasia of duct-like structures. Interstitial fibrosis was observed in 18 cases and was accompanied by adipose tissue infiltration in 15. Decreased lymphoid tissue was observed in 16 samples and oncocytosis was present in 5 cases. We suggest that MFE in edentulous or partially edentulous patients should be considered as an entity for the text books.Tumefações do soalho bucal (TSB são observadas em pacientes edêntulos ou parcialmente edêntulos, prejudicando a adaptação de próteses, e têm sido descritas recentemente na literatura como hiperplasias das glândulas sublinguais. OBJETIVO: O objetivo desse estudo é descrever os aspectos microscópicos das TSB a fim de contribuir para o seu diagnóstico final. MATERIAL E MÉTODOS: Foram removidos cirurgicamente 24 espécimes de 19 pacientes (15 mulheres e 4 homens que possuíam TSB. A idade variou de 48 a 74 anos, com média de 57 anos. A principal

  13. Proposta de um modelo de delimitação geométrica para a injeção ventro-glútea Propuesta de un modelo de delimitación geométrica para inyección ventroglútea A proposal for a geometrical delimitation model for ventro-gluteal injection

    Directory of Open Access Journals (Sweden)

    Abel Silva de Meneses

    2007-10-01

    Full Text Available Este estudo teve por objetivo apresentar e comparar a técnica da delimitação geométrica para aplicação de injeção intramuscular na região ventro-glútea com a tradicional. Trata-se de um estudo quasi-experimental do tipo grupo-controle não-equivalente, realizado em peças anatômicas de cadáveres verificando a precisão da punção, entre a delimitação tradicional, pela mão, e a geométrica, constituída ao traçar linhas imaginárias entre proeminências ósseas do quadril configurando um triângulo, cujo baricentro circunscreverá o sítio de punção. O estudo revelou que o sítio de punção delimitado pela técnica geométrica guardou proporção entre as estruturas envolvidas, coincidiu com o ventre muscular da região ventro-glútea em 100,0% das punções. Já para a tradicional, o sítio de punção se distanciou do ventre muscular em 39,9% das punções.Este estúdio tuvo por objectivo presentar y comparar la técnica de la delimitación geométrica para aplicación de injección intramuscular en la región ventroglútea. Tratase de un estúdio casi-experimental del tipo grupo-control no equivalente realizado con piezas anatómicas de cadaveres verificando la precisión de la punción entre la delimitación tradicional por la mano y la geométrica por la técnica del trazado de lineas imaginárias traves de las proeminencias oseas del quadril configurando un triangulo, cuyo centro es el sitio de punción. Lo estudio demonstró que el sitio de punción delimitado por la técnica geométrica guardó proporción entre las estructuras envolvidas y concidió con el centro muscular de la región ventroglútea en 100% de las punciones. Por otro lado, en la técnica tradicional, el sitio de punción se ha distanciado del centro muscular en 39,9% de las punciones.This study aimed at presenting and comparing geometrical and traditional technique for intramuscular injection in the ventrogluteal area. This is a quasi

  14. Ação da Betametasona em Ratas Prenhes: Impacto sobre os Níveis de Corticosterona e Glândulas Adrenais Maternas e Fetais Effect of Betamethasone on Pregnant Rats: Impact on Corticosterone Level and Maternal and Fetal Adrenal Glands

    Directory of Open Access Journals (Sweden)

    Eduardo de Souza

    2001-12-01

    Full Text Available Objetivo: a utilização repetitiva do corticóide antenatal objetivando acelerar a maturidade pulmonar fetal tem sido muito empregada no risco de parto prematuro, o que nos motivou a estudar a dosagem de corticosterona no termo e aspectos morfológicos das glândulas adrenais maternas e fetais de ratas albinas submetidas à ação da betametasona na segunda metade da prenhez, para verificar conseqüências dessa terapêutica. Métodos: utilizamos 30 ratas prenhes, distribuídas em 3 grupos numericamente iguais. As do Grupo I receberam betametasona nos dias 11, 12, 18 e 19 da prenhez. As do Grupo II receberam água destilada nesses dias (grupo controle, e as do Grupo III não receberam qualquer medicamento, constituindo grupo controle de estresse. Foram todas sacrificadas no 20º dia de prenhez, quando dosamos a corticosterona no sangue das matrizes e extirpamos as glândulas adrenais maternas e fetais para exame de microscopia óptica. Resultados: a dosagem de corticosterona plasmática foi significantemente menor no grupo tratado com betametasona (4,8 mg/dL, quando comparada aos grupos controles (17,7 e 26,8 mg/dL. À microscopia óptica observou-se intensa vacuolização citoplasmática na zona fasciculada das adrenais maternas e fetais no grupo que utilizou a betametasona, indicando intensa supressão adrenal secundária ao uso do medicamento. Conclusões: o uso repetitivo e prolongado de corticóides, em ratas prenhes, para acelerar a maturidade pulmonar fetal determina supressão adrenal materna e fetal.Purpose: the repetitive use of antenatal corticosteroid therapy for acceleration of fetal lung maturation has been common in cases at risk of preterm delivery. We studied the corticosterone levels at term and the morphologic aspects in the maternal and fetal adrenal glands submitted to the effect of betamethasone in the second half of rat pregnancy in order to verify its consequences. Methods: thirty female pregnant rats were divided into

  15. Estudo do estado de diferenciação da célula mioepitelial nas neoplasias de glândula salivar - DOI: 10.4025/actascihealthsci.v26i2.1587 Study of the myoepithelial cell differentiation state in salivary gland tumors - DOI: 10.4025/actascihealthsci.v26i2.1587

    Directory of Open Access Journals (Sweden)

    Ricardo Raitz

    2004-04-01

    Full Text Available A célula mioepitelial (CM nos tumores de glândula salivar apresenta-se em diferentes estágios de diferenciação. Sabe-se que em glândula normal ela expressa actina músculo específica (AME e Citoqueratina (CK 14. Por outro lado, é conhecida a participação dos componentes de matriz extracelular, dentre eles a laminina (LN, na morfogênese e citodiferenciação das estruturas glandulares. Em vista do exposto, nos propusemos a estudar os diferentes estágios de diferenciação da CM através da expressão da AME, da CK 14, bem como a participação da LN neste processo. Para tanto, utilizamos tumores onde se postulam a participação da CM: adenoma pleomórfico, mioepitelioma, adenoma de células basais e carcinoma adenóide cístico e submetemos os espécimes ao método imunohistoquímico da avidina-biotina. Nossos resultados mostraram que a presença da AME foi rara, assim como a CK 14 que só esteve presente em CM de estruturas ductiformes bem formadas. Já a LN esteve presente junto à CM, independentemente da expressão de CK 14 e de AME, e no estroma tanto de tumores diferenciados, como indiferenciados. Em conclusão, é possível identificar diferentes estágios de diferenciação mioepitelial através da expressão da CK 14 e da AME, mas parece não existir uma correlação da LN com a diferenciação da CM tumoral, pois ou essa ou sua precursora continua a secretar LN, mesmo que imperfeitamente após estímulo oncogênico.The myoepithelial cell (MC in salivary gland tumors is presented at different stages of differentiation. The cell presents specific muscles actin (SMA and Citokeratin (CK14 in normal gland. On the other hand, there is a participation of extracellular matrix components, as the laminin (LN, in the morphogenesis and cito differentiation of the glands structures. Considering that, we decided to study the different stages of the MC differentiation through SMA expression, the CK 14, as well as the LN participation in

  16. Doença celíaca: avaliação da obediência à dieta isenta de glúten e do conhecimento da doença pelos pacientes cadastrados na Associação dos Celíacos do Brasil (ACELBRA Celiac disease: evaluation of compliance to a gluten-free diet and knowledge of the disease in celiac patients registered at the Brazilian Celiac Association (BCA

    Directory of Open Access Journals (Sweden)

    Vera Lucia SDEPANIAN

    2001-10-01

    Full Text Available Racional — A obediência à dieta isenta de glúten previne a ocorrência de complicações malignas e não-malignas. Objetivo - Avaliar a obediência à dieta isenta de glúten e o conhecimento teórico acerca da doença celíaca e seu tratamento pelos pacientes cadastrados na Associação dos Celíacos do Brasil (ACELBRA. Métodos - Foi enviado por correio um questionário a respeito da obediência à dieta isenta de glúten e do conhecimento teórico da doença celíaca e seu tratamento a 584 membros cadastrados na ACELBRA. Resultados - Dos 534 (91,4% questionários recebidos, foram analisados 529 (90,6%. Quanto à obediência à dieta, 69,4% dos pacientes responderam que nunca ingerem glúten e 29,5% que não obedecem à dieta. A proporção de pacientes que ingerem glúten freqüentemente ou sem restrição alguma é maior entre aqueles com idade igual ou maior a 21 anos (17,7% do que os com idade menor (9,9%. A freqüência de obediência à dieta foi maior quando o intervalo de tempo em que foi estabelecido o diagnóstico da doença foi inferior a 5 anos. O intestino delgado foi assinalado como o principal órgão afetado na doença celíaca por 82% dos pacientes. Os principais sintomas assinalados foram diarréia (96,6%, emagrecimento (93,4%, barriga inchada (90,4%, anemia (68,1% e vômitos (59,6%. Apenas 59,0% concordaram com a existência de predisposição genética. Segundo 90,4% das respostas, a doença é permanente e 96,2% assinalaram que a dieta deve ser totalmente isenta de glúten; 67,1% responderam que o glúten é uma proteína que está presente, segundo 92,1% dos inquéritos, no trigo, centeio, cevada e aveia. Observou-se maior proporção de obediência à dieta quando há conhecimento da doença e dieta. A biopsia de intestino delgado foi considerada necessária por apenas 67,5% dos pacientes, observando-se maior freqüência de obediência à dieta nos pacientes que realizaram pelo menos uma biopsia de intestino delgado

  17. Estudo da morfo e citodiferenciação da glândula submandibular remanescente de ratos após excisão parcial de um de seus lobos Study of the morpho and cytodifferentiation of the submandibular gland of rats submitted to partial excision of one of its lobes

    Directory of Open Access Journals (Sweden)

    Anna C. M. Fossati

    2004-06-01

    Full Text Available Manter uma boa saúde depende, entre outros fatores, de um fluxo salivar adequado1-3. Os danos causados por obstrução, traumas ou remoção cirúrgica das glândulas salivares poderão acarretar alteração na produção salivar. Qualquer diminuição no fluxo salivar trará conseqüências danosas ao organismo. Desenvolveu-se este trabalho com o objetivo de aprofundar o conhecimento nos mecanismos que envolvem a regeneração da Glândula Submandibular (GSM de ratos submetida à excisão parcial de um de seus lobos. FORMA DE ESTUDO: Experimental. MATERIAL E MÉTODO: Foram utilizadas lâminas referentes ao desenvolvimento glandular de ratos aos 15, 16, 17, 18 e 19 dias de vida fetal, e 20 ratos machos de 30 ou 60 dias. Após conveniente anestesia foi removido o terço inferior do lobo esquerdo da GSM de cada animal. Após a remoção, foram deixados a se recuperar por 2, 3, 7 e 15 dias. Nestes tempos determinados foram eutanaziados, as glândulas removidas, fixadas em Methacarn, incluídas em parafina, e cortes de 5 ¼m efetuados. Foi realizada coloração ou com hematoxilina/eosina ou com ácido Periódico Reativo de Schiff (PAS. RESULTADOS: Observou-se que o processo regenerativo se instalou precocemente e em todos os espécimes estudados. Foi semelhante ao aspecto verificado no desenvolvimento glandular normal e mais acentuado no rato de 30 dias. A citodiferenciação representada pela marcação das mucinas neutras pelo PAS foi discreta inicialmente, logo após atingindo sua produção máxima, então finalmente reduzindo a expressão, sendo mudada a sua localização inicial. CONCLUSÃO: Baseados nos resultados obtidos, concluiu-se que o processo regenerativo da GSM de ratos excisada é estável, permanente e gradual, seguindo etapas definidas.Maintaining good health depends on, amongst other factors, of an adequate salivary flow1,2,3. Damage caused by obstruction, trauma or surgical removal of the salivary glands may lead to alterations

  18. Substituição da proteína do farelo de soja pela proteína do glúten de milho em rações para alevinos de tilápia do Nilo - DOI: 10.4025/actascianimsci.v25i2.1991 Replacement of soybean meal protein by corn gluten meal protein in diets for Nile tilapia fingerlings - DOI: 10.4025/actascianimsci.v25i2.1991

    Directory of Open Access Journals (Sweden)

    Jeisson Emerson Casimiro Ferrari

    2003-04-01

    Full Text Available O experimento foi conduzido com o objetivo de avaliar a substituição da proteína do farelo de soja pela proteína do glúten de milho em rações para alevinos de tilápia do Nilo, Oreochromis niloticus L. (Cichlidae. O delineamento experimental foi inteiramente casualizado com 5 tratamentos 0%; 25%; 50%; 75% e 100% de substituição da proteína do farelo de soja pela proteína do glúten de milho e, com 4 repetições. Os níveis adotados corresponderam a 11,75%; 23%; 35,78% e 47,28% de inclusão de glúten de milho nas rações, as quais foram formuladas para serem isoprotéicas em proteína digestível (PD, isocalóricas em energia digestível (ED e com a mesma quantidade de fibra bruta, lisina e metionina. Foram utilizados 100 alevinos com peso médio de 7,47±1,61g, distribuídos em 20 aquários (250L, em sistema de recirculação de água dotado de controle de temperatura. Foi observado efeito quadrático para o ganho de peso, conversão alimentar e taxa de eficiência protéica, sendo os respectivos valores ótimos estimados em 30,69%; 48% e 46,25% de substituição da proteína do farelo de soja pela proteína do glúten de milho e, para o consumo de ração, foi verificado efeito linear. Em função da média referente aos valores estimados para os diferentes parâmetros avaliados, pôde-se concluir que a proteína do glúten de milho pode substituir até 42% (19,82% de inclusão na ração da proteína do farelo de soja em rações para alevinos de tilápia do Nilo.The research was carried out aiming to evaluate the replacement of soybean meal protein by corn gluten meal protein in Nile tilapia, Oreochromis niloticus L. (Cichlidae diets. The experimental design was completely randomized with five treatments 0%, 25%, 50%, 75% and 100% of replacement of soybean protein by corn gluten meal protein and 4 replicates. The used levels corresponded to 11.75%, 23%, 35.78% and 42.28% of corn gluten meal inclusion in diets, formulated to be

  19. [Humanized childbirth].

    Science.gov (United States)

    Kuo, Su-Chen

    2005-06-01

    Childbirth is a major event in a family. The expectant parent's perception of the childbirth experience influences his or her development as a parent. Making childbirth a positive and satisfying experience for women is the responsibility of health care providers. Women want to have physical and emotional privacy during labor and delivery, and to experience both in a friendly, comfortable environment. For women expected to undergo normal deliveries, humanized childbirth is one accessible approach. This article explores the definition and evolution of humanized childbirth and the care practice that it involves. It also explores birth plans and birth experiences, and the improvements necessary to routine labor practices to enable women to participate in decision making about their childbirth experiences. The author emphasizes that when health-care providers recognize the value of humanized childbirth and make changes accordingly, the dignity of women's childbirth experiences will be enhanced.

  20. Human monkeypox.

    Science.gov (United States)

    McCollum, Andrea M; Damon, Inger K

    2014-01-01

    Human monkeypox is a zoonotic Orthopoxvirus with a presentation similar to smallpox. Clinical differentiation of the disease from smallpox and varicella is difficult. Laboratory diagnostics are principal components to identification and surveillance of disease, and new tests are needed for a more precise and rapid diagnosis. The majority of human infections occur in Central Africa, where surveillance in rural areas with poor infrastructure is difficult but can be accomplished with evidence-guided tools and educational materials to inform public health workers of important principles. Contemporary epidemiological studies are needed now that populations do not receive routine smallpox vaccination. New therapeutics and vaccines offer hope for the treatment and prevention of monkeypox; however, more research must be done before they are ready to be deployed in an endemic setting. There is a need for more research in the epidemiology, ecology, and biology of the virus in endemic areas to better understand and prevent human infections.

  1. Human mimicry

    NARCIS (Netherlands)

    Chartrand, T.L.; Baaren, R.B. van

    2009-01-01

    Human mimicry is ubiquitous, and often occurs without the awareness of the person mimicking or the person being mimicked. First, we briefly describe some of the major types of nonconscious mimicry—verbal, facial, emotional, and behavioral—and review the evidence for their automaticity. Next, we

  2. Practicing Humanities

    DEFF Research Database (Denmark)

    Gimmler, Antje

    2016-01-01

    and self-reflective democracy. Contemporary humanities have adopted a new orientation towards practices, and it is not clear how this fits with the ideals of ‘Bildung’ and ‘pure science’. A possible theoretical framework for this orientation towards practices could be found in John Dewey’s pragmatic...

  3. Human waste

    NARCIS (Netherlands)

    Amin, Md Nurul; Kroeze, Carolien; Strokal, Maryna

    2017-01-01

    Many people practice open defecation in south Asia. As a result, lot of human waste containing nutrients such as nitrogen (N) and phosphorus (P) enter rivers. Rivers transport these nutrients to coastal waters, resulting in marine pollution. This source of nutrient pollution is, however, ignored in

  4. Human Parasites

    OpenAIRE

    Davis, Ryan S.; Hodgson, Erin W.

    2008-01-01

    Entomologists often get “bug” samples for identification, including those that accidentally infest residences. In the United States, we are fortunate to have very few arthropods (e.g., insects, spiders, mites, ticks, etc.) that actually infest or feed on humans.

  5. Human steroidogenesis

    DEFF Research Database (Denmark)

    Andersen, Claus Y; Ezcurra, Diego

    2014-01-01

    reviews current knowledge of the regulation of progesterone in the human ovary during the follicular phase and highlights areas where knowledge remains limited. In this review, we provide in-depth information outlining the regulation and function of gonadotropins in the complicated area of steroidogenesis...

  6. Human Parechoviruses

    DEFF Research Database (Denmark)

    Fischer, Thea Kølsen; Harvala, Heli; Midgley, Sofie

    2017-01-01

    Infections with human parechoviruses (HPeV) are highly prevalent, particularly in neonates, where they may cause substantial morbidity and mortality. The clinical presentation of HPeV infection is often indistinguishable from that of enterovirus (EV) infection and may vary from mild disease...

  7. Nothing Human

    Science.gov (United States)

    Wharram, C. C.

    2014-01-01

    In this essay C. C. Wharram argues that Terence's concept of translation as a form of "contamination" anticipates recent developments in philosophy, ecology, and translation studies. Placing these divergent fields of inquiry into dialogue enables us read Terence's well-known statement "I am a human being--I deem nothing…

  8. Human Trafficking

    Science.gov (United States)

    Wilson, David McKay

    2011-01-01

    The shadowy, criminal nature of human trafficking makes evaluating its nature and scope difficult. The U.S. State Department and anti-trafficking groups estimate that worldwide some 27 million people are caught in a form of forced servitude today. Public awareness of modern-day slavery is gaining momentum thanks to new abolitionist efforts. Among…

  9. Human monkeypox.

    Science.gov (United States)

    Jezek, Z; Gromyko, A I; Szczeniowski, M V

    1983-01-01

    Human monkeypox, occurring in the tropical rainforest of west and central Africa, is regarded as the most important orthopoxvirus infection for epidemiological surveillance during the post-smallpox era. This disease, first recognized in Zaïre in 1970 resembles smallpox clinically but differs epidemiologically. Clinical features, their evolution and sequelae of monkeypox could be compared with discrete ordinary or modified type of smallpox. A case-fatality rate of 14% has been observed but some cases can be exceedingly mild or atypical and may easily remain undetected and unreported. Pronounced lymphadenopathy has been the only clinical feature found commonly in monkeypox but not in smallpox. Fifty-seven cases of human monkeypox have occurred since 1970, in the tropical rainforests in six west and central African countries, the majority of them (45) being reported from Zaïre. The disease appears to be more frequent in dry season. Children below ten years of age comprise 84% of the cases. Smallpox vaccination protects against monkeypox. Clusters of cases have been observed in certain areas within countries and within affected households. Human-to-human spread has possibly occurred seven times. No cases of possible tertiary spread were observed. The secondary attack rate among susceptible close household contacts was 10%, among all susceptible contacts 5%. This is much lower than that occurring with smallpox, which is between 25-40%. The limited avidity of monkeypox virus for human beings indicates that monkeypox is probably a zoonosis, although the animal reservoir(s) have not yet been identified. The low transmissibility, resulting in low frequency of disease in man indicates that monkeypox is not a public health problem. Human monkeypox has been a relatively newly recognized disease. Studies are in progress to identify the natural cycle of monkeypox virus and to define better its clinical and epidemiological characteristics. Special surveillance is maintained in

  10. Human Rights, Human Needs, Human Development, Human Security : Relationships between four international 'human' discourses

    NARCIS (Netherlands)

    D.R. Gasper (Des)

    2007-01-01

    textabstractHuman rights, human development and human security form increasingly important, partly interconnected, partly competitive and misunderstood ethical and policy discourses. Each tries to humanize a pre-existing and unavoidable major discourse of everyday life, policy and politics; each

  11. Human Development Report 2000: Human Rights and Human Development

    OpenAIRE

    United Nations Development Programme, UNDP

    2000-01-01

    The Human Development Report 2000 looks at human rights as an intrinsic part of development—and at development as a means to realizing human rights. It shows how human rights bring principles of accountability and social justice to the process of human development.

  12. Human Rights in the Humanities

    Science.gov (United States)

    Harpham, Geoffrey

    2012-01-01

    Human rights are rapidly entering the academic curriculum, with programs appearing all over the country--including at Duke, Harvard, Northeastern, and Stanford Universities; the Massachusetts Institute of Technology; the Universities of Chicago, of Connecticut, of California at Berkeley, and of Minnesota; and Trinity College. Most of these…

  13. Human Protothecosis

    Science.gov (United States)

    Lass-Flörl, Cornelia; Mayr, Astrid

    2007-01-01

    Human protothecosis is a rare infection caused by members of the genus Prototheca. Prototheca species are generally considered to be achlorophyllic algae and are ubiquitous in nature. The occurrence of protothecosis can be local or disseminated and acute or chronic, with the latter being more common. Diseases have been classified as (i) cutaneous lesions, (ii) olecranon bursitis, or (iii) disseminated or systemic manifestations. Infections can occur in both immunocompetent and immunosuppressed patients, although more severe and disseminated infections tend to occur in immunocompromised individuals. Prototheca wickerhamii and Prototheca zopfii have been associated with human disease. Usually, treatment involves medical and surgical approaches; treatment failure is not uncommon. Antifungals such as ketoconazole, itraconazole, fluconazole, and amphotericin B are the most commonly used drugs to date. Among them, amphotericin B displays the best activity against Prototheca spp. Diagnosis is largely made upon detection of characteristic structures observed on histopathologic examination of tissue. PMID:17428884

  14. Human paleoneurology

    CERN Document Server

    2015-01-01

    The book presents an integrative review of paleoneurology, the study of endocranial morphology in fossil species. The main focus is on showing how computed methods can be used to support advances in evolutionary neuroanatomy, paleoanthropology and archaeology and how they have contributed to creating a completely new perspective in cognitive neuroscience. Moreover, thanks to its multidisciplinary approach, the book addresses students and researchers approaching human paleoneurology from different angles and for different purposes, such as biologists, physicians, anthropologists, archaeologists

  15. Human Cloning

    Science.gov (United States)

    2006-07-20

    research group, headed by Douglas Melton and Kevin Eggan, submitted their proposal to a Harvard committee composed of ethicists, scientists and public...United States. Although the company offered no proof of its claim, Dr . Brigette Boisselier, Managing Director of Clonaid, stated that genetic tests would...a year of the Dolly announcement, concerns over human cloning were heightened when Dr . Richard Seed, a Chicago scientist, announced on January 7

  16. Human universe

    CERN Document Server

    Cox, Brian

    2014-01-01

    Human life is a staggeringly strange thing. On the surface of a ball of rock falling around a nuclear fireball in the blackness of a vacuum the laws of nature conspired to create a naked ape that can look up at the stars and wonder where it came from. What is a human being? Objectively, nothing of consequence. Particles of dust in an infinite arena, present for an instant in eternity. Clumps of atoms in a universe with more galaxies than people. And yet a human being is necessary for the question itself to exist, and the presence of a question in the universe - any question - is the most wonderful thing. Questions require minds, and minds bring meaning. What is meaning? I don't know, except that the universe and every pointless speck inside it means something to me. I am astonished by the existence of a single atom, and find my civilisation to be an outrageous imprint on reality. I don't understand it. Nobody does, but it makes me smile. This book asks questions about our origins, our destiny, and our place i...

  17. Human Being Human: Culture and the Soul

    OpenAIRE

    Hauke, Chris

    2005-01-01

    Human Being Human explores the classical question 'What is a human being?'\\ud \\ud In examining our human being, Christopher Hauke challenges the notion of human nature, questions the assumed superiority of human consciousness and rational thinking and pays close attention to the contradiction of living simultaneously as an autonomous individual and a member of the collective community. The main chapters include:\\ud \\ud who's in charge here?\\ud knowledge power and human being\\ud that thinking ...

  18. Substituição do milho por farelo de trigo ou farelo de glúten de milho na ração de bovinos de corte em terminação = Substitution of corn grain by wheat middlings or corn gluten feed in the finishing bulls diet

    Directory of Open Access Journals (Sweden)

    Eduardo Menegueli Pereira

    2007-01-01

    Full Text Available O objetivo do trabalho foi avaliar a substituição do milho moído fino (MM por farelo de trigo (FT ou farelo de glúten de milho (FGM na ração de 36 bovinos não castrados, em terminação (14 meses; peso inicial de 382 kg durante 87 dias. A ração continha 24% de silagem de capim e 76% de concentrado, constituído por polpa cítrica, farelo de soja, uréia, minerais, bicarbonato de sódio e monensina sódica, além de uma das fontes energéticas testadas. A substituição de MM por FT aumentou a ingestão de FDN (pThe objective of this work was to evaluate the substitution of finely ground (GC corn by wheat middlings (WM or dried corn gluten feed (CGF in finishing feedlot diets. Thirty-six yearling bulls (382 kg average initial live weight; 14 months old were fed during 87 days. The diets contained 24% grass silage and 76% concentrate, formulated with dried citrus pulp, soybean meal, urea, mineral-vitamin mix,sodium bicarbonate, sodium monensin, and one of the energy sources tested. The substitution of wheat middlings for corn increased NDF intake (p<0.05. However, no effect was observed on other measured parameters. Substitution of corn gluten feed for ground corn reduced dry matter intake (p<0.05 and average daily gain (p<0.10, increasedNDF intake (p<0.05, but had no effect on feed efficiency. These diets showed no differences in the observed values of net energy of maintenance or gain. The ratio observed/expected net energy for the diet differs from the other treatments. For this type of diet, GC may be completely replaced by WM or CGF with no depression on feed efficiency or on diet net energy. NRC (2001 underestimates TDN values and net energy estimates for WM and CGF.

  19. Comparison of green pit viper and Agkistrodon halys antivenom in inhibition of coagulopathy due to Trimeresurus albolabris venom: an in-vitro study using human plasma.

    Science.gov (United States)

    Lam, S K; Yip, S F; Crow, P; Fung, H T; Cheng, J Mh; Tan, K S; Wong, O F; Yeung, D Yt; Wong, Y K; Poon, K M; Ades, G

    2017-02-01

    There are two antivenoms that may be administered in Hong Kong following a bite by Trimeresurus albolabris: the green pit viper antivenom from the Thai Red Cross Society in Thailand and the Agkistrodon halys antivenom from the Shanghai Institute of Biological Products in China. Both are recommended by the Central Coordinating Committee of Accident and Emergency Services of the Hospital Authority for treating patients with a bite by Trimeresurus albolabris. The choice of which antivenom to use is based on physician preference. This study aimed to compare the relative efficacy of the two antivenoms. This in-vitro experimental study was carried out by a wildlife conservation organisation and a regional hospital in Hong Kong. Human plasma from 40 adult health care worker volunteers was collected. The Trimeresurus albolabris venom was added to human plasma and the mixture was assayed after incubation with each antivenom (green pit viper and Agkistrodon halys) using saline as a control. Fibrinogen level and clotting time in both antivenom groups were studied. The mean fibrinogen level was elevated from 0 g/L to 2.86 g/L and 1.11 g/L after the addition of green pit viper antivenom and Agkistrodon halys antivenom, respectively. When mean clotting time was measured, the value was 6.70 minutes in the control, prolonged to more than 360 minutes by green pit viper antivenom and to 19.06 minutes by Agkistrodon halys antivenom. Green pit viper antivenom was superior to Agkistrodon halys antivenom in neutralisation of the thrombin-like and hypofibrinogenaemic activities of Trimeresurus albolabris venom.

  20. Introduction: Digital Humanities, Public Humanities

    Directory of Open Access Journals (Sweden)

    Alex Christie

    2014-07-01

    Full Text Available NANO: New American Notes Online: An Interdisciplinary Academic Journal for Big Ideas in a Small World. This special issue shows how both public and digital humanities research can be rendered more persuasive through engagement with cultures beyond the academy. More specifically, the aim of this special issue is to demonstrate how investments in technologies and computation are not necessarily antithetical to investments in critical theory and social justice.

  1. Molecular epidemiology of human rabies viruses in Sri Lanka.

    Science.gov (United States)

    Matsumoto, Takashi; Ahmed, Kamruddin; Karunanayake, Dushantha; Wimalaratne, Omala; Nanayakkara, Susilakanthi; Perera, Devika; Kobayashi, Yuji; Nishizono, Akira

    2013-08-01

    Rabies is a lethal zoonotic disease caused by the rabies virus, which is transmitted by rabid animals to humans. Rabies is prevalent in all continents, with over 60% of human deaths occurring in Asia. Sri Lanka is a rabies-endemic country. This study shows that rabies afflicted more older individuals than children in Sri Lanka between 2008 and 2010. This novel finding indicates that older people in Sri Lanka should be more aware of the risk of rabies. Phylogenetic analyses of the rabies N and G genes showed that the Sri Lankan rabies viruses are distinct and probably originated from a single clone. The G-L noncoding region is highly diverse, and is suitable for the analysis of virus evolution within a country. A phylogenetic analysis of this region showed high diversity in the currently circulating Sri Lankan rabies viruses, which can be divided into seven clades. Some clades are unique to a specific geographic region, whereas others occur at multiple locations. This indicates that the movement of dogs, the main rabies-transmitting animal in Sri Lanka, is restricted in some areas but less limited in others. These data may help to formulate a more efficient rabies control program in Sri Lanka. Copyright © 2013 Elsevier B.V. All rights reserved.

  2. Human Computing, Virtual Humans and Artificial Imperfection

    NARCIS (Netherlands)

    Ruttkay, Z.M.; Reidsma, Dennis; Nijholt, Antinus; Quek, F.; Yang, Y.

    2006-01-01

    In this paper we raise the issue whether imperfections, characteristic of human-human communication, should be taken into account when developing virtual humans. We argue that endowing virtual humans with the imperfections of humans can help making them more ‘comfortable’ to interact with. That is,

  3. Human Capital, (Human) Capabilities and Higher Education

    Science.gov (United States)

    Le Grange, L.

    2011-01-01

    In this article I initiate a debate into the (de)merits of human capital theory and human capability theory and discuss implications of the debate for higher education. Human capital theory holds that economic growth depends on investment in education and that economic growth is the basis for improving the quality of human life. Human capable…

  4. Alterações funcionais e histológicas das glândulas paratireóides após derivação jejuno-cólica: estudo comparativo em ratos Functional and histologic alterations of the parathyroid glands after jejunocolic bypass: a compartive study in rats

    Directory of Open Access Journals (Sweden)

    Mario Castro Carreiro

    1997-06-01

    Full Text Available Com a finalidade de estudar as glândulas paratireóides durante a fase de redução ponderal, 48 ratos Wistar, machos, foram distribuídos em dois grupos. Os animais do Grupo1, no total de 28, foram submetidos à derivação jejuno-cólica. Os animais do Grupo2, no total de 20, foram submetidos à operação simulada. Estudaram-se, à microscopia óptica, os aspectos histológicos, e à microscopia eletrônica, os aspectos ultraestruturais. A função das glândulas paratireóides foi estudada mediante determinações séricas do Hormônio da Paratireóide - Parato-hormônio (PTH, Cálcio, Fosfato, Albumina e Proteínas Totais. Procedeu-se à avaliação da evolução ponderal e variação percentual, pesando-se os animais nos dias 7, 14, 21 e 28. A determinação sérica do PTH, Cálcio, Fosfato, Albumina e Proteínas Totais foi realizada antes dos sacrifícios e os estudados da microscopia, após os mesmos, nos 7o, 14o, 21o e 28o dias. Os valores do peso, da variação percentual e das dosagens bioquímicas e hormonais foram submetidos à análise estatística separadamente para cada grupo. Compararam-se também os Grupos 1 e 2 em relação aos valores das variáveis estudadas nos 7o, 14o, 21o e 28o dias do sacrifício. Os animais submetidos à derivação jejuno-cólica apresentaram progressiva perda ponderal demonstrada a partir do sétimo dia e os animais de controle, crescente ganho de peso a partir do 14o dia. A média dos valores das determinações séricas do PTH dos ratos submetidos à derivação jejuno-cólica foi maior do que dos animais-controle já a partir do 7o dia, embora estatisticamente significantes. Os valores séricos do fosfato só foram estatisticamente significantes no 21o dia, o da albumina no 14o, 21o e 28o dias e os das proteínas totais em todos os períodos. A média dos valores do cálcio foi menor nos animais-controle dos que nos submetidos à derivação jejuno-cólica, não sendo esse valores estatisticamente

  5. Humanizing Architecture

    DEFF Research Database (Denmark)

    Toft, Tanya Søndergaard

    2015-01-01

    The article proposes the urban digital gallery as an opportunity to explore the relationship between ‘human’ and ‘technology,’ through the programming of media architecture. It takes a curatorial perspective when proposing an ontological shift from considering media facades as visual spectacles...... agency and a sense of being by way of dematerializing architecture. This is achieved by way of programming the symbolic to provide new emotional realizations and situations of enlightenment in the public audience. This reflects a greater potential to humanize the digital in media architecture....

  6. Think Human

    DEFF Research Database (Denmark)

    Nielsen, Charlotte Marie Bisgaard

    2013-01-01

    The paper probes the background of the dire rhetoric of the Danish National Health Board’s 40 week anti-alcohol consumption campaign, in particular the model of communication implied by the campaign's strategy. Contrasting the campaign's strategy in 2011 with the results of evaluations of previous...... years' campaigns suggests that the theory of communication underlying the campaign has its basis in mechanical action rather than in human communication. The practice of 'Communication design' is investigated in relation to this metaphorical 'machine thinking' model of communication and contrasted...

  7. Cystic echinococcosis in Algeria: cattle act as reservoirs of a sheep strain and may contribute to human contamination.

    Science.gov (United States)

    Bardonnet, K; Benchikh-Elfegoun, M C; Bart, J M; Harraga, S; Hannache, N; Haddad, S; Dumon, H; Vuitton, D A; Piarroux, R

    2003-08-29

    In Algeria, cystic echinococcosis (CE) is a serious economic and public health problem. The common sheep/dog cycle is usually considered as the major source of human contamination. But to date the main strain of Echinococcus granulosus involved in the human contamination and the role of other hosts are still unknown. This paper reports an original work performed in northern Algeria combining field observations and molecular analysis. In a first step, examination of 6237 carcasses in slaughterhouses showed high infection and fertility rates in cattle and dromedaries. Then, in a second step, we used a molecular biology approach to identify the E. granulosus strain(s) involved. Forty-six samples from various origins were collected. They were analysed using comparison of PCR-amplified DNA sequences with one genomic (BG 1/3) and two mitochondrial (COI and NDI) targets. Results show the presence of a "sheep" strain of E. granulosus in North Algeria circulating between cattle and ovines and infectious to humans, whereas in South Algeria, a "camel" strain and a "sheep" strain were found to circulate in camels and in sheep, respectively. This study also reports an ambiguous genotype which resembled the "sheep" strain genotype (Gl) on the basis of the partial COI gene sequence, whereas on the basis of the partial NDI gene sequence, it was similar either to the "sheep" strain (Gl) or to the "camel" strain (G6). Besides its basic interest, our study confirms the role of other hosts (mainly cattle) in leading to transmission to humans and suggests that control measures should not only target sheep.

  8. Does low IgA in human milk predispose the infant to development of cow's milk allergy?

    Science.gov (United States)

    Järvinen, K M; Laine, S T; Järvenpää, A L; Suomalainen, H K

    2000-10-01

    We sought a relationship between total and cow's milk-specific IgA levels in colostrum and human milk and subsequent development of cow's milk allergy (CMA) in the breast-fed infant. The study included 87 nursing mothers and their infants (age, 2 d to 7 mo), followed prospectively up to 1 y. At 1 y, 48 mothers (69% with an atopic constitution) had an infant with CMA, verified by clinical cow's milk challenge, eight (38% with an atopic constitution) had a baby who had had protracted infantile colic but no CMA (disease control group), and 31 (23% with an atopic constitution) had a healthy infant. Total breast-milk IgA was measured by radial immunodiffusion, and IgA antibodies to cow's milk were measured by ELISA during the breast-feeding period. The levels of total and cow's milk-specific IgA antibodies in colostrum and human milk were significantly lower in the mothers whose baby later developed CMA [estimated third day value, 0.38 g/L (95% confidence interval, 0. 24-0.82)] than in the ones whose infant remained healthy or had had infantile colic but not CMA [0.82 g/L (95% confidence interval, 0. 99-1.51); p milk was cow's milk-specific IgA positively correlated with the levels of total IgA but not with the development of CMA in the infant. The levels of total or cow's milk-specific IgA did not correlate with maternal atopy. IgA antibodies in colostrum and human milk may prevent antigen entry at the intestinal surface of the breast-fed infant. A low IgA content in human milk may lead to defective exclusion of food antigens and thus predispose an offspring to develop food allergies.

  9. Ultraviolet-C Irradiation: A Novel Pasteurization Method for Donor Human Milk.

    Directory of Open Access Journals (Sweden)

    Lukas Christen

    Full Text Available Holder pasteurization (milk held at 62.5°C for 30 minutes is the standard treatment method for donor human milk. Although this method of pasteurization is able to inactivate most bacteria, it also inactivates important bioactive components. Therefore, the objective of this study was to investigate ultraviolet irradiation as an alternative treatment method for donor human milk.Human milk samples were inoculated with five species of bacteria and then UV-C irradiated. Untreated and treated samples were analysed for bacterial content, bile salt stimulated lipase (BSSL activity, alkaline phosphatase (ALP activity, and fatty acid profile.All five species of bacteria reacted similarly to UV-C irradiation, with higher dosages being required with increasing concentrations of total solids in the human milk sample. The decimal reduction dosage was 289±17 and 945±164 J/l for total solids of 107 and 146 g/l, respectively. No significant changes in the fatty acid profile, BSSL activity or ALP activity were observed up to the dosage required for a 5-log10 reduction of the five species of bacteria.UV-C irradiation is capable of reducing vegetative bacteria in human milk to the requirements of milk bank guidelines with no loss of BSSL and ALP activity and no change of FA.

  10. Ultraviolet-C Irradiation: A Novel Pasteurization Method for Donor Human Milk.

    Science.gov (United States)

    Christen, Lukas; Lai, Ching Tat; Hartmann, Ben; Hartmann, Peter E; Geddes, Donna T

    2013-01-01

    Holder pasteurization (milk held at 62.5°C for 30 minutes) is the standard treatment method for donor human milk. Although this method of pasteurization is able to inactivate most bacteria, it also inactivates important bioactive components. Therefore, the objective of this study was to investigate ultraviolet irradiation as an alternative treatment method for donor human milk. Human milk samples were inoculated with five species of bacteria and then UV-C irradiated. Untreated and treated samples were analysed for bacterial content, bile salt stimulated lipase (BSSL) activity, alkaline phosphatase (ALP) activity, and fatty acid profile. All five species of bacteria reacted similarly to UV-C irradiation, with higher dosages being required with increasing concentrations of total solids in the human milk sample. The decimal reduction dosage was 289±17 and 945±164 J/l for total solids of 107 and 146 g/l, respectively. No significant changes in the fatty acid profile, BSSL activity or ALP activity were observed up to the dosage required for a 5-log10 reduction of the five species of bacteria. UV-C irradiation is capable of reducing vegetative bacteria in human milk to the requirements of milk bank guidelines with no loss of BSSL and ALP activity and no change of FA.

  11. Ultraviolet-C Irradiation: A Novel Pasteurization Method for Donor Human Milk

    Science.gov (United States)

    Christen, Lukas; Lai, Ching Tat; Hartmann, Ben; Hartmann, Peter E.; Geddes, Donna T.

    2013-01-01

    Background Holder pasteurization (milk held at 62.5°C for 30 minutes) is the standard treatment method for donor human milk. Although this method of pasteurization is able to inactivate most bacteria, it also inactivates important bioactive components. Therefore, the objective of this study was to investigate ultraviolet irradiation as an alternative treatment method for donor human milk. Methods Human milk samples were inoculated with five species of bacteria and then UV-C irradiated. Untreated and treated samples were analysed for bacterial content, bile salt stimulated lipase (BSSL) activity, alkaline phosphatase (ALP) activity, and fatty acid profile. Results All five species of bacteria reacted similarly to UV-C irradiation, with higher dosages being required with increasing concentrations of total solids in the human milk sample. The decimal reduction dosage was 289±17 and 945±164 J/l for total solids of 107 and 146 g/l, respectively. No significant changes in the fatty acid profile, BSSL activity or ALP activity were observed up to the dosage required for a 5-log10 reduction of the five species of bacteria. Conclusion UV-C irradiation is capable of reducing vegetative bacteria in human milk to the requirements of milk bank guidelines with no loss of BSSL and ALP activity and no change of FA. PMID:23840820

  12. Modulation of bacterial translocation in mice mediated through lactose and human milk oligosaccharides.

    Science.gov (United States)

    Mielcarek, C; Romond, P C; Romond, M B; Bezirtzoglou, E

    2011-12-01

    Massive resection of the small intestine in infants is imposed to the regulation of several intestinal pathological situations, as intestinal adaptation cannot be relied upon. Many nutritional disturbances are occurring following surgery procedure. In this vein, long-term parenteral feeding is adopt to improve prognosis not always successfully. Clostridia and more specifically Clostridium perfringens, are suspected to participate in the physiopathology of the rising situation. In order to investigate the effect of lactose and human milk neutral oligosaccharides (HMNOs) on Clostridia, germfree mice were inoculated either with enterotoxigenic C.perfringens strain isolated from a patient with NEC, or with a human microbiota harboring C.clostridioforme group(HF). In this vein, different doses of lactose were administrated during 2 weeks in adult mice on an attempt to evaluate the lactase activity. Intake of lactose (70 g/L) and HMNOs (7 g/L) in C.perfringens monoassociated mice induced mortality within a week. In HF mice, no mortality was observed. An increase in Clostridia occurrence was observed in the median ileum after intake of 7 g lactose (p = 0.017). Higher clostridial numbers occurred in caecum following intake of 70 g lactose (p drinking lower lactose concentrations. Finally, bacteremia was more frequent in 70 g lactose/L mice (p milk oligosaccharides could favor clostridial population when reaching the lower intestine. The shortness of the small intestine in infants underwent massive intestinal resection seems to be associated to an incomplete breakdown of lactose. Enteral feeds formulas deprived in lactose would be more suitable in enteral feeding of infants. Copyright © 2011 Elsevier Ltd. All rights reserved.

  13. Análise acústica da voz captada na faringe próximo à fonte glótica através de microfone acoplado ao fibrolaringoscópio Acoustic analysis of voice captured in the pharynx above the glottic source through a microphone on a laryngo-fiberscope

    Directory of Open Access Journals (Sweden)

    Erica E. Fukuyama

    2001-01-01

    Full Text Available Objetivo: O objetivo deste trabalho foi estudar a voz próximo à sua fonte produtora, as pregas vocais, através de um microfone miniaturizado de aparelho auditivo que foi adaptado para ser acoplado à extremidade de um fibrolaringoscópio, permitindo a captação da voz durante a laringoscopia direta. Forma de estudo: Experimental. Material e Método: A voz foi estudada em um grupo de 50 indivíduos, 25 homens e 25 mulheres sem doenças, através de um programa de análise acústica MDVP (Multi-Dimensional Voice Program do laboratório de voz Computerized Speech Lab, Model 4300B, da Kay Elemetrics. Amostras de vogais sustentadas /a/, /i/ e /u/ foram captadas de três formas diferentes, primeiramente com um microfone comum externo a 15 cm da boca, em segundo lugar com o microfone especial na faringe a 1,5 cm acima das pregas vocais e por último com o microfone especial externamente a 2 cm da boca. Doze parâmetros acústicos relacionados a freqüência fundamental, amplitude e ruído de cada uma das vogais foram comparadas estatisticamente conforme à sua forma de captação. Resultados: Os resultados mostraram diferenças estatisticamente significativas entre a voz captada pelo microfone comum externo e o microfone especial, em relação à freqüência fundamental, aos parâmetros de variação de periodicidade de freqüência, amplitude e ruído. Conclusão: A diferença do som da fonte glótica do som da voz externa pode mostrar as modificações sofridas pela voz no decorrer da passagem pelo trato vocal.Aim: The aim of the present study is to examine the voice to its acoustic source - the vocal folds - with a miniature hearing-aid microphone coupled to the extremity of a laryngo-fiberscope allowing the voice to be captured during direct laryngoscopy. Study design: Experimental. Material and Method: The voice of 50 individuals - 25 males and 25 females bearing no pathologies - was collected by the Multi-Dimensional Voice Program (MDVP by

  14. Influência da glândula pineal sobre a ingestão de água e NaCl em ratas normais e ooforectomizadas Influence of oophorectomy and pinealectomy on water and NaCl ingestion by adult rats

    Directory of Open Access Journals (Sweden)

    José Maria Soares Jr.

    1999-07-01

    Full Text Available Objetivo: avaliar a influência da pineal sobre a ingestão de água e de NaCl em ratas normais e ooforectomizadas. Métodos: utilizaram-se 48 (n = 48 ratas adultas, divididas ao acaso em quatro grupos: GI - mantidas sem manipulação, servindo como controle (n = 20; GII - submetidas à ooforectomia bilateral (n = 8; GIII - submetidas à pinealectomia (n = 12; GIV - submetidas à ooforectomia bilateral e à pinealectomia (n = 8. A seguir, todos os animais foram mantidos em gaiolas individuais; após três semanas. Determinou-se diariamente a fase do ciclo por esfregaço vaginal e anotou-se diariamente o volume ingerido de água e NaCl a 0,25 M, por aproximadamente três meses. Resultados: 1 as ratas submetidas somente à pinealectomia apresentaram maior freqüência da fase de estro, sendo que algumas entraram em estado de estro permanente; 2 o consumo de líquidos (água e solução salina não se alterou durante as diferentes fases do ciclo estral; 3 as ratas do grupo submetido à ooforectomia tiveram maior consumo de água, sendo que a pinealectomia nesses animais restabeleceu o consumo normal de água; 4 os animais ooforectomizados e os ooforectomizados e pinealectomizados mostraram redução do consumo médio de solução salina. Conclusões: os dados obtidos sugerem que a glândula pineal poderia modular a ação dos esteróides ovarianos sobre a ingestão de sal e água em ratas adultas.Purpose: to evaluate the effects of oophorectomy and pinealectomy on the ingestion of water and NaCl solution by adults female rats. Methods: forty-eight adult virgin female rats (Wistar EPM 1 weighing 200 g were kept on routine laboratory care and fed water and Purina rat chow ad libitum. The animals were random by divided into four groups: GI - maintained without manipulation as a control group (n = 20; GII - submitted to bilateral oophorectomy (n = 8; GIII - submitted to pinealectomy (n = 12; GIV - submitted to bilateral oophorectomy and pinealectomy (n

  15. Bases fisiológicas para una interacción entre las células cromafines y las endoteliales de la glándula adrenal Physiological bases for an interaction between chromaffin and endothelial cells from the adrenal gland

    Directory of Open Access Journals (Sweden)

    MARIO LUXORO

    2001-03-01

    Full Text Available En este trabajo tratamos de investigar las posibles interacciones entre las células endoteliales de la glándula adrenal y aquellas sustancias relacionadas con la secreción de las células cromafines. Para lo anterior, estudiamos el efecto de acetilcolina (ACh, o de catecolaminas (CA tanto en el nivel de Ca2+ citoplasmático ([Ca2+]i, como en el potencial de membrana de las células endoteliales. Nuestros resultados muestran que tanto la ACh como la nicotina, pero no la muscarina, son capaces de inducir un aumento del [Ca2+]i y una despolarización de la membrana plasmática de las células endoteliales. El antagonista nicotínico, hexametonium, bloquea tanto el efecto de la ACh como de la nicotina lo que sugiere la presencia de receptores nicotínicos. Por otra parte, las CA (tanto adrenalina como noradrenalina o agonistas a1-adrenérgicos también producen un aumento del [Ca2+]i en las células endoteliales aunque no despolarización evidente. En este caso, el aumento es bifásico siendo la primera fase de un pico rápido e independiente del Ca2+ extracelular en tanto que la segunda se presenta con oscilaciones y depende tanto de que los canales de Ca2+ no estén bloqueados como de la presencia de ese ión en el medio externo. Dado que se ha demostrado que el aumento del [Ca2+]i en las células endoteliales desencadena la secreción de sustancias vasodilatadoras (prostaciclina y óxido nítrico, proponemos que éste sería un mecanismo compensatorio del sistema para contrarestar el enorme efecto vasoconstrictor de las CA secretadas por las células cromafinesIn this work we investigated the possible interactions between the endothelial cells from the adrenal gland and the substances related with the secretion from the chromaffin cells. In order to do so, we studied the effect of acetyl-choline (ACh or of catecholamines (CA on the level of the membrane potential and the cytoplasmic concentration of free calcium ([Ca2+]i in the endothelial

  16. Avaliação da superexpressão da proteína p53 e das mutações no éxon 8 do gene TP53 em carcinomas mamários caninos e glândulas normais

    Directory of Open Access Journals (Sweden)

    Maria Juliana D. Teixeira

    2011-06-01

    Full Text Available Este estudo foi realizado com o objetivo de avaliar a expressão da proteína p53, pela técnica de imuno-histoquímica, em neoplasmas mamários malignos em cadelas, além de investigar mutações no éxon 8 do gene supressor Tp53 por meio do padrão de bandas obtidas por PCR-RFLP. Dezenove mamas de cadelas saudáveis foram usadas como controle (Grupo 1. Amostras de 18 casos de tumores malignos (Grupo 2 e suas glândulas mamárias contralaterais (Grupo 3 foram obtidas na rotina do Hospital Veterinário da UFRPE. Os tumores foram identificados histologicamente e classificados em graus de malignidade. O método da estreptoavidina-biotina peroxidase foi utilizado para a análise da expressão de p53 por imuno-histoquímica, de acordo com a localização e intensidade da coloração. A expressão da proteína p53 não foi observada nas amostras do Grupo 1, mas foi encontrada em todas as amostras de tumores malignos (Grupo 2 seja só no núcleo, ou também no citoplasma. No Grupo 3, a expressão foi observada em quatro amostras normais e em duas que apresentavam tumor. Para a análise molecular, o DNA genômico foi extraído e submetido à PCR-RFLP com as seguintes endonucleases: AluI, BsoBI, DdeI e SmaI. O padrão de bandas foi polimórfico entre os grupos, mas não entre as variantes tumorais. Esse polimorfismo detectou mutações no fragmento estudado - éxon 8 do gene Tp53 - que podem resultar em alterações nos nucleotídeos, localizados nos sítios de restrição das enzimas. Esses achados levam a conclusão de que a imunoexpressão da p53 não tem relação com o subtipo histológico ou grau de malignidade do tumor, mas sim com a presença dos tumores no tecido mamário de cadelas. A PCR-RFLP pode ser usada como importante ferramenta para o estudo da carcinogênese mamária na cadela, possibilitando gerar diagnósticos precoces através do polimorfismo obtido com endonucleases de restrição pré-selecionadas.

  17. The high-level expression of human tissue plasminogen activator in the milk of transgenic mice with hybrid gene locus strategy.

    Science.gov (United States)

    Zhou, Yanrong; Lin, Yanli; Wu, Xiaojie; Xiong, Fuyin; Lv, Yuemeng; Zheng, Tao; Huang, Peitang; Chen, Hongxing

    2012-02-01

    Transgene expression for the mammary gland bioreactor aimed at producing recombinant proteins requires optimized expression vector construction. Previously we presented a hybrid gene locus strategy, which was originally tested with human lactoferrin (hLF) as target transgene, and an extremely high-level expression of rhLF ever been achieved as to 29.8 g/l in mice milk. Here to demonstrate the broad application of this strategy, another 38.4 kb mWAP-htPA hybrid gene locus was constructed, in which the 3-kb genomic coding sequence in the 24-kb mouse whey acidic protein (mWAP) gene locus was substituted by the 17.4-kb genomic coding sequence of human tissue plasminogen activator (htPA), exactly from the start codon to the end codon. Corresponding five transgenic mice lines were generated and the highest expression level of rhtPA in the milk attained as to 3.3 g/l. Our strategy will provide a universal way for the large-scale production of pharmaceutical proteins in the mammary gland of transgenic animals.

  18. Human Factors Planning Guidelines

    Science.gov (United States)

    1996-01-01

    To ensure human factors considerations are fully incorporated in the system : development, the Integrated Product Team (IPT) or Program Manager initiates a : Human Factors Program (HFP) that addresses the human performance and human : resource parame...

  19. Heterogeneous hCG and hMG commercial preparations result in different intracellular signalling but induce a similar long-term progesterone response in vitro.

    Science.gov (United States)

    Riccetti, Laura; Klett, Danièle; Ayoub, Mohammed Akli; Boulo, Thomas; Pignatti, Elisa; Tagliavini, Simonetta; Varani, Manuela; Trenti, Tommaso; Nicoli, Alessia; Capodanno, Francesco; La Sala, Giovanni Battista; Reiter, Eric; Simoni, Manuela; Casarini, Livio

    2017-10-01

    Are four urinary hCG/menotropin (hMG) and one recombinant preparation characterized by different molecular features and do they mediate specific intracellular signaling and steroidogenesis? hCG and hMG preparations have heterogeneous compositions and mediate preparation-specific cell signaling and early steroidogenesis, although similar progesterone plateau levels are achieved in 24 h-treated human primary granulosa cells in vitro. hCG is the pregnancy hormone marketed as a drug for ARTs to induce final oocyte maturation and ovulation, and to support FSH action. Several hCG formulations are commercially available, differing in source, purification methods and biochemical composition. Commercial hCG preparations for ART or research purposes were compared in vitro. The different preparations were quantified by immunoassay with calibration against the hCG standard (Fifth IS; NIBSC 07/364). Immunoreactivity patterns, isoelectric points and oligosaccharide contents of hCGs were evaluated using reducing and non-reducing Western blotting, capillary isoelectric-focusing immunoassay and lectin-ELISA, respectively. Functional studies were performed in order to evaluate intracellular and total cAMP, progesterone production and β-arrestin 2 recruitment by ELISA and BRET, in both human primary granulosa lutein cells (hGLC) and luteinizing hormone (LH)/hCG receptor (LHCGR)-transfected HEK293 cells, stimulated by increasing hormone concentrations. Statistical analysis was performed using two-way ANOVA and Bonferroni post-test or Mann-Whitney's U-test as appropriate. Heterogeneous profiles were found among preparations, revealing specific molecular weight patterns (20-75 KDa range), isoelectric points (4.0-9.0 pI range) and lectin binding (P hCG/hMG preparations is provided in International Units (IU) by in-vivo bioassay and calibration against an International Standard, although it is an unsuitable unit of measure for in-vitro studies. The re-calibration against recombinant h

  20. The Digital Humanities as a Humanities Project

    Science.gov (United States)

    Svensson, Patrik

    2012-01-01

    This article argues that the digital humanities can be seen as a humanities project in a time of significant change in the academy. The background is a number of scholarly, educational and technical challenges, the multiple epistemic traditions linked to the digital humanities, the potential reach of the field across and outside the humanities,…

  1. NATO Human View Architecture and Human Networks

    Science.gov (United States)

    Handley, Holly A. H.; Houston, Nancy P.

    2010-01-01

    The NATO Human View is a system architectural viewpoint that focuses on the human as part of a system. Its purpose is to capture the human requirements and to inform on how the human impacts the system design. The viewpoint contains seven static models that include different aspects of the human element, such as roles, tasks, constraints, training and metrics. It also includes a Human Dynamics component to perform simulations of the human system under design. One of the static models, termed Human Networks, focuses on the human-to-human communication patterns that occur as a result of ad hoc or deliberate team formation, especially teams distributed across space and time. Parameters of human teams that effect system performance can be captured in this model. Human centered aspects of networks, such as differences in operational tempo (sense of urgency), priorities (common goal), and team history (knowledge of the other team members), can be incorporated. The information captured in the Human Network static model can then be included in the Human Dynamics component so that the impact of distributed teams is represented in the simulation. As the NATO militaries transform to a more networked force, the Human View architecture is an important tool that can be used to make recommendations on the proper mix of technological innovations and human interactions.

  2. Digital Humanities

    DEFF Research Database (Denmark)

    Nielsen, Hans Jørn

    2015-01-01

    overgangen fra trykkekultur til digital kultur. For det første problemstillingen omkring digitalisering af litterær kulturarv med fokus på kodning og tagging af teksten samt organisering i hypertekststrukturer. For det andet reorganiseringen af det digitale dokument i dataelementer og database. For det......Artiklen præsenterer først nogle generelle problemstillinger omkring Digital Humanities (DH) med det formål at undersøge dem nærmere i relation til konkrete eksempler på forskellige digitaliseringsmåder og ændringer i dokumentproduktion. I en nærmere afgrænsning vælger artiklen den tendens i DH......, der betragter DH som forbundet med "making" og "building" af digitale objekter og former. Dette kan også karakteriseres som DH som praktisk-produktiv vending. Artiklen har valgt tre typer af digitalisering. De er valgt ud fra, at de skal repræsentere forskellige måder at håndtere digitaliseringen på...

  3. Human Rhinoviruses

    Science.gov (United States)

    Lamson, Daryl M.; St. George, Kirsten; Walsh, Thomas J.

    2013-01-01

    Human rhinoviruses (HRVs), first discovered in the 1950s, are responsible for more than one-half of cold-like illnesses and cost billions of dollars annually in medical visits and missed days of work. Advances in molecular methods have enhanced our understanding of the genomic structure of HRV and have led to the characterization of three genetically distinct HRV groups, designated groups A, B, and C, within the genus Enterovirus and the family Picornaviridae. HRVs are traditionally associated with upper respiratory tract infection, otitis media, and sinusitis. In recent years, the increasing implementation of PCR assays for respiratory virus detection in clinical laboratories has facilitated the recognition of HRV as a lower respiratory tract pathogen, particularly in patients with asthma, infants, elderly patients, and immunocompromised hosts. Cultured isolates of HRV remain important for studies of viral characteristics and disease pathogenesis. Indeed, whether the clinical manifestations of HRV are related directly to viral pathogenicity or secondary to the host immune response is the subject of ongoing research. There are currently no approved antiviral therapies for HRVs, and treatment remains primarily supportive. This review provides a comprehensive, up-to-date assessment of the basic virology, pathogenesis, clinical epidemiology, and laboratory features of and treatment and prevention strategies for HRVs. PMID:23297263

  4. A Validated RP-HPLC Method for the Determination of Recombinant Human Insulin in Bulk and Pharmaceutical Dosage Form

    Directory of Open Access Journals (Sweden)

    B. A. Moussa

    2010-01-01

    Full Text Available A modified RP-HPLC method was developed for the quantitative determination of recombinant human insulin in bulk and pharmaceutical dosage form with reduced retention time. Study of the effects of the column temperature, pH of the mobile phase and presence of vial additives (phenol and m-cresol, or impurities (A-21 Disamido on the accuracy of the assay were assessed. Separation was achieved using a Hypersil BDS C-18 column and the mobile phase was composed of solution A (aqueous solution of 28.3 anhydrous Na2SO4g/L, pH 2.3 and solution B (28.5 g anhydrous Na2SO4 g/L in 50:50 mixture of water and acetonitrile, pH 2.3 in a ratio 48:52 (v/v at 45–50 °C. The column temperature was 40 °C, the flow rate was 1 mL/min and detection was performed at 216 nm. The procedures were validated according to international conference on harmonization (ICH guidelines. Recovery study was done applying standard addition technique for further validation of the procedure. The retention time of recombinant human insulin was 19.7 min as compared to 29 min obtained by the reference method. Analytical conditions fluctuations or presence of vial additives or impurities did not show any significant effect on the accuracy of the method. The prepared standard insulin solution in 0.01 N HCl was found to be stable for 5 days. Statistical comparison showed no significant difference between the described method and reference method regarding the accuracy and precision. The modified method can be applied for routine quality control applications for determination of recombinant human insulin.

  5. Cattle mammary bioreactor generated by a novel procedure of transgenic cloning for large-scale production of functional human lactoferrin.

    Directory of Open Access Journals (Sweden)

    Penghua Yang

    Full Text Available Large-scale production of biopharmaceuticals by current bioreactor techniques is limited by low transgenic efficiency and low expression of foreign proteins. In general, a bacterial artificial chromosome (BAC harboring most regulatory elements is capable of overcoming the limitations, but transferring BAC into donor cells is difficult. We describe here the use of cattle mammary bioreactor to produce functional recombinant human lactoferrin (rhLF by a novel procedure of transgenic cloning, which employs microinjection to generate transgenic somatic cells as donor cells. Bovine fibroblast cells were co-microinjected for the first time with a 150-kb BAC carrying the human lactoferrin gene and a marker gene. The resulting transfection efficiency of up to 15.79 x 10(-2 percent was notably higher than that of electroporation and lipofection. Following somatic cell nuclear transfer, we obtained two transgenic cows that secreted rhLF at high levels, 2.5 g/l and 3.4 g/l, respectively. The rhLF had a similar pattern of glycosylation and proteolytic susceptibility as the natural human counterpart. Biochemical analysis revealed that the iron-binding and releasing properties of rhLF were identical to that of native hLF. Importantly, an antibacterial experiment further demonstrated that rhLF was functional. Our results indicate that co-microinjection with a BAC and a marker gene into donor cells for somatic cell cloning indeed improves transgenic efficiency. Moreover, the cattle mammary bioreactors generated with this novel procedure produce functional rhLF on an industrial scale.

  6. Prophylactic and postexposure efficacy of a potent human monoclonal antibody against MERS coronavirus

    Science.gov (United States)

    Corti, Davide; Zhao, Jincun; Pedotti, Mattia; Simonelli, Luca; Agnihothram, Sudhakar; Fett, Craig; Fernandez-Rodriguez, Blanca; Foglierini, Mathilde; Agatic, Gloria; Vanzetta, Fabrizia; Gopal, Robin; Langrish, Christopher J.; Barrett, Nicholas A; Sallusto, Federica; Baric, Ralph S.; Varani, Luca; Zambon, Maria; Perlman, Stanley; Lanzavecchia, Antonio

    2015-01-01

    Middle East Respiratory Syndrome (MERS) is a highly lethal pulmonary infection caused by a previously unidentified coronavirus (CoV), likely transmitted to humans by infected camels. There is no licensed vaccine or antiviral for MERS, therefore new prophylactic and therapeutic strategies to combat human infections are needed. In this study, we describe, for the first time, to our knowledge, the isolation of a potent MERS-CoV–neutralizing antibody from memory B cells of an infected individual. The antibody, named LCA60, binds to a novel site on the spike protein and potently neutralizes infection of multiple MERS-CoV isolates by interfering with the binding to the cellular receptor CD26. Importantly, using mice transduced with adenovirus expressing human CD26 and infected with MERS-CoV, we show that LCA60 can effectively protect in both prophylactic and postexposure settings. This antibody can be used for prophylaxis, for postexposure prophylaxis of individuals at risk, or for the treatment of human cases of MERS-CoV infection. The fact that it took only 4 mo from the initial screening of B cells derived from a convalescent patient for the development of a stable chinese hamster ovary (CHO) cell line producing neutralizing antibodies at more than 5 g/L provides an example of a rapid pathway toward the generation of effective antiviral therapies against emerging viruses. PMID:26216974

  7. Usage analysis of human serum albumin in patients with liver cancer and liver cirrhosis after hepatectomy

    Directory of Open Access Journals (Sweden)

    HUANG Donghai

    2015-06-01

    Full Text Available ObjectiveTo analyze the usage of human serum albumin in patients with liver cancer and liver cirrhosis after hepatectomy. MethodsA total of 121 patients with liver cancer and liver cirrhosis who received hepatectomy in our hospital from January 2012 to January 2014 were divided into control group (n=60 and observation group (n=61. Both groups received human serum albumin in addition to the routine treatment for liver protection. The observation group was given intravenous drip of 5% human serum albumin within 48 h after surgery. The plasma albumin concentrations of patients were measured at 48 h after surgery, and if the concentration was <35 g/L, the patients would be given 20% human serum albumin until the concentration was ≥35 g/L. The control group was given intravenous drip of 20% human serum albumin within 48 h after surgery until the plasma albumin concentration was ≥35 g/L. The amounts of used human serum albumin and plasma were recorded for both groups. The urine volume, abdominal drainage volume, central venous pressure (CVP, mean arterial pressure (MAP, and thromboelastogram (TEG R and K values were measured at 1, 3, 7, and 10 days after surgery. The liver function indices before and after surgery and the indocyanine green retention rate at 15 minutes (ICG R15 at 7 days after surgery were measured. Comparison of continuous data between the two groups was made by t test, while comparison of categorical data was made by chisquare test. Results(1 There were no significant differences in age, sex, Child-Pugh classification, surgical approach, intraoperative blood loss, occlusion time of the first porta hepatis, and operation time between the two groups (P>0.05. But there were significant differences in the amounts of used human serum albumin and plasma and the length of hospital stay between the two groups (P<0.05. (2 There were significant differences in daily urine volume, CVP, MAP, abdominal drainage volume, and interstitial

  8. Human Factors in Human-Systems Integration

    Science.gov (United States)

    Fitts, David J.; Sandor, Aniko; Litaker, Harry L., Jr.; Tillman, Barry

    2008-01-01

    Any large organization whose mission is to design and develop systems for humans, and train humans needs a well-developed integration and process plan to deal with the challenges that arise from managing multiple subsystems. Human capabilities, skills, and needs must be considered early in the design and development process, and must be continuously considered throughout the development lifecycle. This integration of human needs within system design is typically formalized through a Human-Systems Integration (HSI) program. By having an HSI program, an institution or organization can reduce lifecycle costs and increase the efficiency, usability, and quality of its products because human needs have been considered from the beginning.

  9. Analysis of the safety and pharmacodynamics of human fibrinogen concentrate in animals

    Energy Technology Data Exchange (ETDEWEB)

    Beyerle, Andrea, E-mail: andrea.beyerle@cslbehring.com [CSL Behring GmbH, Preclinical Research and Development, Marburg (Germany); Nolte, Marc W. [CSL Behring GmbH, Preclinical Research and Development, Marburg (Germany); Solomon, Cristina [CSL Behring GmbH, Medical Affairs, Marburg (Germany); Department of Anaesthesiology, Perioperative Medicine and General Intensive Care, Paracelsus Medical University, Salzburg (Austria); Herzog, Eva; Dickneite, Gerhard [CSL Behring GmbH, Preclinical Research and Development, Marburg (Germany)

    2014-10-01

    Fibrinogen, a soluble 340 kDa plasma glycoprotein, is critical in achieving and maintaining hemostasis. Reduced fibrinogen levels are associated with an increased risk of bleeding and recent research has investigated the efficacy of fibrinogen concentrate for controlling perioperative bleeding. European guidelines on the management of perioperative bleeding recommend the use of fibrinogen concentrate if significant bleeding is accompanied by plasma fibrinogen levels less than 1.5–2.0 g/l. Plasma-derived human fibrinogen concentrate has been available for therapeutic use since 1956. The overall aim of the comprehensive series of non-clinical investigations presented was to evaluate i) the pharmacodynamic and pharmacokinetic characteristics and ii) the safety and tolerability profile of human fibrinogen concentrate Haemocomplettan P® (RiaSTAP®). Pharmacodynamic characteristics were assessed in rabbits, pharmacokinetic parameters were determined in rabbits and rats and a safety pharmacology study was performed in beagle dogs. Additional toxicology tests included: single-dose toxicity tests in mice and rats; local tolerance tests in rabbits; and neoantigenicity tests in rabbits and guinea pigs following the introduction of pasteurization in the manufacturing process. Human fibrinogen concentrate was shown to be pharmacodynamically active in rabbits and dogs and well tolerated, with no adverse events and no influence on circulation, respiration or hematological parameters in rabbits, mice, rats and dogs. In these non-clinical investigations, human fibrinogen concentrate showed a good safety profile. This data adds to the safety information available to date, strengthening the current body of knowledge regarding this hemostatic agent. - Highlights: • A comprehensive series of pre-clinical investigations of human fibrinogen concentrate. • Human fibrinogen concentrate was shown to be pharmacodynamically active. • Human fibrinogen concentrate was well tolerated

  10. Expressão de galectina-3 e citoqueratina 19 nas neoplasias epiteliais da glândula tireóidea e correlação histopatológica Expression of galectin-3 and cytokeratin 19 in the epithelial neoplasm of the thyroid gland and histopathological correlation

    Directory of Open Access Journals (Sweden)

    Marilene Paladino Rosa

    2005-02-01

    Full Text Available INTRODUÇÃO: O câncer da glândula tireóidea é caracterizado pela sua variedade clínica e patológica. O difícil diagnóstico pré-operatório das lesões foliculares induz a mais procedimentos cirúrgicos de caráter diagnóstico do que propriamente terapêutico. A proposta deste estudo foi identificar a expressão imuno-histoquímica das neoplasias epiteliais tireóideas utilizando anticorpos monoclonais para galectina-3 e citoqueratina 19 e correlacioná-la com variáveis histopatológicas. MATERIAL E MÉTODO: A expressão da galectina-3 e da citoqueratina 19 foi estudada imuno-histoquimicamente em 84 casos com diagnóstico de tecido normal (n = 10, bócio adenomatoso (n = 8, adenoma folicular (n = 12, adenoma de célula de Hürthle (n = 3, carcinoma papilífero (n = 29, carcinoma folicular (n = 8, carcinoma insular (n = 4, carcinoma de células de Hürthle (n = 4 e carcinoma indiferenciado (n = 6, selecionados a partir de pacientes operados no Serviço de Cirurgia de Cabeça e Pescoço do Hospital Heliópolis (HOSPHEL, no período de 1984 a 1995. RESULTADOS: A expressão da galectina-3 foi observada em bócio adenomatoso (12,5%, adenoma folicular (16,7%, carcinoma papilífero (96,6%, carcinoma folicular (12,5%, carcinoma insular (50%, carcinoma de célula de Hürthle (100% e carcinoma indiferenciado (50%. Nossos resultados confirmaram a expressão significativa da galectina-3 no grupo das neoplasias malignas, principalmente no carcinoma papilífero. O padrão de expressão da citoqueratina 19 foi diferente entre os tipos de lesão: enquanto nos bócios e adenomas mostrou-se fraco, nos carcinomas papilíferos foi forte e difuso. CONCLUSÃO: Associadas, a galectina-3 e a citoqueratina 19 auxiliam o diagnóstico histopatológico, principalmente nas lesões de interpretação duvidosa, além de evidenciarem atipias e, com isso, determinarem uma lesão suspeita.BACKGROUND: The cancer of the thyroid gland is characterized by its clinical and

  11. Electromyographic preactivation pattern of the gluteus medius during weight-bearing functional tasks in women with and without anterior knee pain Padrão de pré-ativação eletromiográfica do glúteo médio durante atividades funcionais com descarga de peso em mulheres com e sem dor anterior do joelho

    Directory of Open Access Journals (Sweden)

    Theresa H. Nakagawa

    2011-02-01

    Full Text Available BACKGROUND: Proximal factors have been proposed to influence the biomechanics of the patellofemoral joint. A delayed or diminished gluteus medius (GM activation, before the foot contact on the ground during functional activities could lead to excessive femur adduction and internal rotation and be associated with anterior knee pain (AKP. There are few studies on this topic and the results were inconclusive, therefore, it is necessary to investigate the GM preactivation pattern during functional activities. OBJECTIVE: To compare the GM electromyographic (EMG preactivation pattern during walking, descending stairs and in single leg jump task in women with and without AKP. METHODS: Nine women clinically diagnosed with AKP and ten control subjects with no history of knee injury participated in this study. We evaluated GM EMG linear envelope before the foot contact on the ground during walking and GM onset time and EMG linear envelope during descending stairs as well as in a single leg vertical jump. Mann-Whitney U tests were used to determine the between-group differences in GM EMG preactivation pattern. RESULTS: No between-group differences were observed in GM linear envelope during walking (P=0.41, GM onset time and linear envelope during descending stairs (P=0.17 and P=0.15 and single leg jump (P=0.81 and P=0.33. CONCLUSIONS: Women with AKP did not demonstrated altered GM preactivation pattern during functional weight bearing activities. Our results did not support the hypothesis that poor GM preactivation pattern could be associated with AKP.CONTEXTUALIZAÇÃO: Tem sido proposto que fatores proximais influenciam a biomecânica da articulação fêmoro-patelar. Um atraso ou diminuição da ativação do glúteo médio (GM antes do contato do pé no solo, durante atividades funcionais, poderia levar a adução e rotação interna excessiva do fêmur e provocar a dor anterior do joelho (DAJ. Existem poucos estudos sobre o assunto, e os resultados n

  12. Expressão imuno-histoquímica de p53 na discriminação do comportamento biológico dos tumores de glândulas salivares Immunohistochemical expression of p53 on the biological behavior of salivary gland tumors

    Directory of Open Access Journals (Sweden)

    Kelen Christine do Nascimento Souza

    2005-06-01

    Full Text Available INTRODUÇÃO: Mutações no gene TP53 têm sido identificadas em várias neoplasias, incluindo as de glândulas salivares (NGS. Entretanto não existe consenso sobre o significado biológico da expressão de p53 nessas lesões. OBJETIVOS: Avaliar a marcação imuno-histoquímica do antígeno p53 em NGS, investigando possíveis diferenças entre lesões benignas e malignas, entre diferentes tipos histológicos e entre tumores malignos que: 1 não desenvolveram metástases; 2 emitiram metástases; e 3 metástases de NGS. MATERIAL E MÉTODO: Foram avaliados 16 casos de adenomas pleomórficos (AP, 17 lesões malignas que não apresentaram metástase durante a proservação (sete carcinomas adenóides císticos [CAC] e dez mucoepidermóides [CM], 13 tumores malignos que emitiram metástases (nove CAC e quatro CM e, ainda, 12 metástases regionais ou distantes (cinco CAC e sete CM. Por marcação imuno-histoquímica (estreptavidina-biotina-peroxidase, índices médios de localização do antígeno p53 foram estabelecidos para cada tumor. Para análise, utilizaram-se testes de Mann-Whitney e Kruskal-Wallis, com nível de significância de 5%. RESULTADOS: Foram observadas diferenças estatisticamente significantes nos índices médios de imunomarcação de p53 entre lesões benignas e malignas (p = 0,03 e entre os diferentes tipos histológicos, notadamente entre o AP e o CM (p = 0,02. Todavia houve marcada sobreposição dos intervalos de índices de expressão entre os grupos testados. Nenhuma diferença de expressão foi observada entre as neoplasias malignas quanto ao seu comportamento metastático. CONCLUSÃO: Embora a expressão de p53 tenha sido detectada em todos os subtipos de NGS, a marcação imuno-histoquímica deste antígeno não permitiu discriminar os tumores malignos quanto ao seu comportamento biológico.BACKGROUND: Mutations in the tumor suppressor gene TP53 have been found in many tumors, including salivary gland neoplasms (SGN

  13. Humane Education: An Overview.

    Science.gov (United States)

    Whitlock, Eileen S.; Westerlund, Stuart R.

    This booklet traces the historical development of human education as it has been instilled into the young people of America from colonial times to the present and provides a future prognosis of humaneness in the schools. Humane education promotes humane behavior and is an important part of the humane movement in the United States, although until…

  14. IVI human factors strategy

    Science.gov (United States)

    1999-11-01

    This document focuses on human factors research that supports the Intelligent Vehicle Initiative (IVI). The status of the problem areas within categories used often by the human factors community to organize human factors process is discussed. A simi...

  15. Human Factors Job Aid

    Science.gov (United States)

    1996-12-09

    The purpose of this Human Factors Job Aid is to serve as a desk reference for : human factors integration during system acquisition. The first chapter contains : an overview of the FAA human factors process in system acquisitions. The : remaining eig...

  16. The golden triangle of human dignity: human security, human development and human rights

    NARCIS (Netherlands)

    Gaay Fortman, B. de

    2004-01-01

    The success or failure of processes of democratization cannot be detached from processes of development related to the aspirations of people at the grassroots. Human rights, in a more theoretical terminology, require human development in order to enhance human security.

  17. Insulin-increased L-arginine transport requires A(2A adenosine receptors activation in human umbilical vein endothelium.

    Directory of Open Access Journals (Sweden)

    Enrique Guzmán-Gutiérrez

    Full Text Available Adenosine causes vasodilation of human placenta vasculature by increasing the transport of arginine via cationic amino acid transporters 1 (hCAT-1. This process involves the activation of A(2A adenosine receptors (A(2AAR in human umbilical vein endothelial cells (HUVECs. Insulin increases hCAT-1 activity and expression in HUVECs, and A(2AAR stimulation increases insulin sensitivity in subjects with insulin resistance. However, whether A(2AAR plays a role in insulin-mediated increase in L-arginine transport in HUVECs is unknown. To determine this, we first assayed the kinetics of saturable L-arginine transport (1 minute, 37°C in the absence or presence of nitrobenzylthioinosine (NBTI, 10 µmol/L, adenosine transport inhibitor and/or adenosine receptors agonist/antagonists. We also determined hCAT-1 protein and mRNA expression levels (Western blots and quantitative PCR, and SLC7A1 (for hCAT-1 reporter promoter activity. Insulin and NBTI increased the extracellular adenosine concentration, the maximal velocity for L-arginine transport without altering the apparent K(m for L-arginine transport, hCAT-1 protein and mRNA expression levels, and SLC7A1 transcriptional activity. An A2AAR antagonist ZM-241385 blocked these effects. ZM241385 inhibited SLC7A1 reporter transcriptional activity to the same extent in cells transfected with pGL3-hCAT-1(-1606 or pGL3-hCAT-1(-650 constructs in the presence of NBTI + insulin. However, SLC7A1 reporter activity was increased by NBTI only in cells transfected with pGL3-hCAT-1(-1606, and the ZM-241385 sensitive fraction of the NBTI response was similar in the absence or in the presence of insulin. Thus, insulin modulation of hCAT-1 expression and activity requires functional A(2AAR in HUVECs, a mechanism that may be applicable to diseases associated with fetal insulin resistance, such as gestational diabetes.

  18. Human-machine interactions

    Science.gov (United States)

    Forsythe, J Chris [Sandia Park, NM; Xavier, Patrick G [Albuquerque, NM; Abbott, Robert G [Albuquerque, NM; Brannon, Nathan G [Albuquerque, NM; Bernard, Michael L [Tijeras, NM; Speed, Ann E [Albuquerque, NM

    2009-04-28

    Digital technology utilizing a cognitive model based on human naturalistic decision-making processes, including pattern recognition and episodic memory, can reduce the dependency of human-machine interactions on the abilities of a human user and can enable a machine to more closely emulate human-like responses. Such a cognitive model can enable digital technology to use cognitive capacities fundamental to human-like communication and cooperation to interact with humans.

  19. y Human herpesvirus 6 envelope components enriched in lipid rafts: evidence for virion-associated lipid rafts

    Directory of Open Access Journals (Sweden)

    Yamanishi Koichi

    2009-08-01

    Full Text Available Abstract In general, enveloped viruses are highly dependent on their lipid envelope for entry into host cells. Here, we demonstrated that during the course of virus maturation, a significant proportion of human herpesvirus 6 (HHV-6 envelope proteins were selectively concentrated in the detergent-resistant glycosphingolipid- and cholesterol-rich membranes (rafts in HHV-6-infected cells. In addition, the ganglioside GM1, which is known to partition preferentially into lipid rafts, was detected in purified virions, along with viral envelope glycoproteins, gH, gL, gB, gQ1, gQ2 and gO indicating that at least one raft component was included in the viral particle during the assembly process.

  20. Evaluation of the Dermal Bioavailability of Aqueous Xylene in F344 Rats and Human Volunteers

    Energy Technology Data Exchange (ETDEWEB)

    Thrall, Karla D.(BATTELLE (PACIFIC NW LAB)); Woodstock, Angie D.(BATTELLE (PACIFIC NW LAB))

    2003-07-11

    Xylene is a clear, colorless liquid used as a solvent in the printing, rubber, and leather industries and is commonly found in paint thinners, paints, varnishes, and adhesives. Although humans are most likely to be exposed to xylene via inhalation, xylene is also found in well and surface water. Therefore, an assessment of the dermal contribution to total xylene uptake is useful for understanding human exposures. To evaluate the significance of these exposures, the dermal absorption of o-xylene was assessed in F344 male rats and human volunteers using a combination of real-time exhaled breath analysis and physiologically based pharmacokinetic (PBPK) modeling. Animals were exposed to o-xylene dermally. Immediately following the initiation of exposure, individual animals were placed in a glass off-gassing chamber and exhaled breath was monitored. Human volunteers participating in the study placed both legs into a stainless steel hydrotherapy tub containing an initial concentration of approximately 500 g/L o-xylene. Exhaled breath was continually analyzed from each volunteer before, during, and post-exposure to track absorption and subsequent elimination of the compound in real time. In both animal and human studies, a PBPK model was used to estimate the dermal permeability coefficient (Kp) to describe each set of exhaled breath data. Rat skin was found to be approximately 12 times more permeable to aqueous o-xylene than human skin. The estimated human and rat aqueous o-xylene Kp values were 0.005+/- 0.001 cm/hr and 0.058+/- 0.009 cm/hr, respectively.

  1. Production of human recombinant bone morphogenetic protein-2A by high density culture of Escherichia coli with stationary dissolved oxygen fed-batch condition.

    Science.gov (United States)

    Li, M; Chen, C; Pu, Q; Chen, S

    1998-01-01

    The optimization of cultivation condition in 500 ml shake flasks was carried out to produce recombinant human bone morphogenetic protein-2A (BMP-2A) in recombinant Escherichia coli YK537/pDH-B2m, followed by a 5L fermenter batch and condition-controlled fed-batch culture to obtain BMP-2A. The comparison of these two methods indicated that cultivation by keeping dissolved oxygen at 30%-40% and limiting glucose concentration could obtain BMP-2A 2.78 g/L broth, the final cell density was OD600 53 (dry cell weight 21.2 g/L), and expressed BMP-2A was 25% of the total amount of protein in E.coli. The critical fermentation conditions included: (1) keeping appropriate dissolved oxygen concentration in the process; (2) limiting glucose concentration; (3) taking heat induction at the middle-log phase and maintaining 42 degrees C for 4 hours; (4) controlling specific growth rate around 0.3 h-1 in the duration of growth.

  2. Direct detection of fibrinogen in human plasma using electric-double-layer gated AlGaN/GaN high electron mobility transistors

    Science.gov (United States)

    Regmi, Abiral; Sarangadharan, Indu; Chen, Yen-Wen; Hsu, Chen-Pin; Lee, Geng-Yen; Chyi, Jen-Inn; Shiesh, Shu-Chu; Lee, Gwo-Bin; Wang, Yu-Lin

    2017-08-01

    Fibrinogen found in blood plasma is an important protein biomarker for potentially fatal diseases such as cardiovascular diseases. This study focuses on the development of an assay to detect plasmatic fibrinogen using electrical double layer gated AlGaN/GaN high electron mobility transistor biosensors without complex sample pre-treatment methods used in the traditional assays. The test results in buffer solution and clinical plasma samples show high sensitivity, specificity, and dynamic range. The sensor exhibits an ultra-low detection limit of 0.5 g/l and a detection range of 0.5-4.5 g/l in 1× PBS with 1% BSA. The concentration dependent sensor signal in human serum samples demonstrates the specificity to fibrinogen in a highly dense matrix of background proteins. The sensor does not require complicated automation, and quantitative results are obtained in 5 min with <5 μl sample volume. This sensing technique is ideal for speedy blood based diagnostics such as POC (point of care) tests, homecare tests, or personalized healthcare.

  3. Expression and characterization of bioactive recombinant human alpha-lactalbumin in the milk of transgenic cloned cows.

    Science.gov (United States)

    Wang, J; Yang, P; Tang, B; Sun, X; Zhang, R; Guo, C; Gong, G; Liu, Y; Li, R; Zhang, L; Dai, Y; Li, N

    2008-12-01

    Improvement of the nutritional value of cow milk with transgenic expression of recombinant human alpha-lactalbumin (alpha-LA) has been previously attempted. However, the detailed characterization of the recombinant protein and analysis of the transgenic milk components are not explored yet. Here, we first report production of healthy transgenic cows by somatic cell nuclear transfer, in which expression of up to 1.55 g/L of recombinant human alpha-LA was achieved. The recombinant human alpha-LA was purified from transgenic milk and displayed physicochemical properties similar to its natural counterpart with respect to molecular weight, structure, and regulatory activity for beta-1,4-galactosyltransferase. Additionally, no N-glycosylation was found in the recombinant human alpha-LA, whereas the endogenous bovine alpha-LA was glycosylated at the unusual site (71)Asn-Ile-(73)Cys. Compared with milk from nontransgenic cows, expression of the transgene did not materially alter milk composition, such as fat and protein content. Our research thus provides scientific evidence supporting the feasibility of humanizing cow milk.

  4. Generation of bi-transgenic pigs overexpressing human lactoferrin and lysozyme in milk.

    Science.gov (United States)

    Cui, Dan; Li, Jia; Zhang, Linlin; Liu, Shen; Wen, Xiao; Li, Qiuyan; Zhao, Yaofeng; Hu, Xiaoxiang; Zhang, Ran; Li, Ning

    2015-04-01

    Intensive swine production industry uses antibiotics to treat diseases and improve pig growth. This can not only cause antibiotic resistance, but can also pollute the environment or eventually affect human public health. To date, human lactoferrin (hLF) and human lysozyme (hLZ) have been known as non-adaptive but interactive antimicrobial members and could act in concert against bacteria, which contribute to host defense. Therefore, their expression in pigs might be an alternative strategy for replacing antibiotics in the pig production industry. In our study, we produced hLF and hLZ bi-transgenic pigs and assessed the milk's antibacterial ability. Integration of both transgenes was confirmed by PCR and southern blot. Both the hLF and hLZ were expressed in the mammary gland of bi-transgenic pigs, as detected by western blotting. The expression amounts were 6.5 g/L for hLF and 1.1 mg/L for hLZ using ELISA. Interestingly, pig milk containing hLF and hLZ had synergistic antimicrobial activity. Our results suggest an alternative approach for avoiding the use of antibiotics in the pig industry, which would be of great benefit to the commercial swine production.

  5. Lipid content and fatty acids composition of mature human milk in rural North China.

    Science.gov (United States)

    Wan, Zhong-Xiao; Wang, Xiao-Li; Xu, Li; Geng, Qian; Zhang, Yumei

    2010-03-01

    To determine the lipid content and fatty acid (FA) composition of breast milk from fifty-two lactating women between ninth and twelfth lactation weeks in rural North China. The mothers were questioned on their dietary habits. Total milk lipids extracts were transmethylated and analysed using GLC to determine FA contents. The mean lipid content was 40.21 (sd 1.43) g/l. SFA constituted 35.92 % of the total FA. Medium-chain and long-chain SFA presented levels of 10.91 and 25.01 %, respectively. MUFA and PUFA constituted 32.59 and 19.97 % of the total FA, respectively. Oleic, linoleic and alpha-linolenic acid (ALA) presented contents of 31.26, 17.73 and 1.03 %, respectively. Arachidonic acid had a content of 0.30 %, while DHA content was 0.19 %. Not any form of trans FA were found in human milk samples. A maternal diet transition is proceeding in China. Further investigation on the analysis of human milk FA composition is needed to upgrade the human milk database in China.

  6. [Effects of matrine on proliferation and apoptosis of human renal cell carcinoma cell line GRC-1].

    Science.gov (United States)

    Chong, Tie; Niu, Jian-Qiang; Wang, Zi-Ming; She, Jun-Jun; Huang, Chen

    2006-07-01

    To observe the effects of matrine on proliferation and apoptosis of human renal cell carcinoma cell line GRC-1 in vitro, and to explore its mechanism. The human renal cell carcinoma cell line GRC-1 was treated with matrine of different concentrations for 24, 48, 72 and 96 h respectively. The MTT assay was used to evaluate the cytotoxic effects of matrine on GRC-1 cells. The transmission electron microscope and flow cytometry were utilized to observe and detect the apoptosis of GRC-1 cells induced by matrine. The expression levels of Bcl-2 and Bax proteins were evaluated by streptavidin-biotin-peroxidase method. The matrine of different concentrations all have cytotoxic effects on GRC-1 cells, with obvious dose- and time-dependent effects. The apoptosis induced by matrine was confirmed in GRC-1 cells. With intervention of matrine (1.5 g/L) for 12 h, the expression level of Bcl-2 in GRC-1 cells was decreased while the expression level of Bax was increased as compared with those in the untreated group. The proliferation-inhibiting effects of matrine on human renal cell carcinoma cell line GRC-1 may be related to down-regulating the ratio of Bcl-2/Bax protein expression and promoting the apoptosis.

  7. Special Section: Human Rights

    Science.gov (United States)

    Frydenlund, Knut; And Others

    1978-01-01

    Eleven articles examine human rights in Europe. Topics include unemployment, human rights legislation, role of the Council of Europe in promoting human rights, labor unions, migrant workers, human dignity in industralized societies, and international violence. Journal available from Council of Europe, Directorate of Press and Information, 67006…

  8. ISS Payload Human Factors

    Science.gov (United States)

    Ellenberger, Richard; Duvall, Laura; Dory, Jonathan

    2016-01-01

    The ISS Payload Human Factors Implementation Team (HFIT) is the Payload Developer's resource for Human Factors. HFIT is the interface between Payload Developers and ISS Payload Human Factors requirements in SSP 57000. ? HFIT provides recommendations on how to meet the Human Factors requirements and guidelines early in the design process. HFIT coordinates with the Payload Developer and Astronaut Office to find low cost solutions to Human Factors challenges for hardware operability issues.

  9. Injeção de gordura na prega vocal: efeitos do local de injeção sobre a configuração glótica e a distribuição espacial da gordura injetada Fat injection into the vocal fold: effects of the place of injection on the configuration of the glottis and the spatial distribution of the fat

    Directory of Open Access Journals (Sweden)

    Rui Imamura

    2003-08-01

    Full Text Available A injeção intracordal de gordura tem sido utilizada para medializar a prega vocal em casos de paralisia laríngea, com resultados variáveis. A ausência de consenso entre os autores em relação a diversos aspectos técnicos, como o local de injeção, pode contribuir, em parte, para essa variação. OBJETIVO: Estudar o efeito do local de injeção em relação à distribuição espacial da gordura dentro da prega vocal e à configuração glótica após essas injeções. FORMA DE ESTUDO: Experimental. MATERIAL E MÉTODO: Seis laringes adultas excisadas foram utilizadas. Gordura colhida por lipoaspiração foi injetada, por meio de uma seringa de pressão, no ponto médio da prega vocal em três laringes e lateralmente ao processo vocal da aritenóide em outras três. Fotografias digitais em vista cranial da glote foram obtidas antes e após as injeções. Após fixação e descalcificação, as laringes foram cortadas no plano frontal nos terços anterior, médio e posterior da prega vocal e no nível da cartilagem aritenóide, para estudar a distribuição da gordura. RESULTADOS: Somente a injeção de gordura lateralmente ao processo vocal da aritenóide permitiu o fechamento da glote posterior. A gordura tendeu a ocupar um espaço cilíndrico dentro do músculo tireoaritenóideo, ao longo do maior eixo da prega vocal, mesmo quando injetada próximo ao processo vocal. CONCLUSÕES: O local de injeção da gordura teve influência sobre a configuração glótica após a injeção, apesar da gordura tender a ocupar um espaço cilíndrico ao longo da prega vocal em ambos os grupos.Intracordal fat injection has been used for vocal fold medialization in cases of laryngeal paralysis with variable results. A lack of consensus among the authors concerning various technical aspects, such as the site of injection, may contribute, in part, to this variation. AIM: To study the effects of the place of fat injection on the spatial distribution of fat and

  10. Biochemical analysis of the living human vitreous.

    Science.gov (United States)

    Kokavec, Jan; Min, San H; Tan, Mei H; Gilhotra, Jagjit S; Newland, Henry S; Durkin, Shane R; Grigg, John; Casson, Robert J

    2016-09-01

    To date, our understanding of the biochemical composition of the living human vitreous relies on extrapolations from animal or human post-mortem studies. This was a cross-sectional study of vitreous samples from 27 individuals scheduled for retinal surgery within a tertiary hospital. From each vitreous sample, the concentrations of sodium, potassium, chloride, calcium, magnesium, glucose, lactate, β- hydroxybutyrate, copper, zinc, selenium, iron, ferritin and transferrin and osmolality were measured. Perioperative serum samples were also obtained for comparison. The following vitreous mean ± standard deviation (95% confidence interval of the mean) was observed for each analyte: sodium, 146.7 ± 3.3 (145.4-148.0) mmol/L; potassium, 5.73 ± 0.86 (5.39-6.08) mmol/L; chloride, 121.6 ± 2.6 (120.6-122.7) mmol/L; calcium, 1.128 ± 0.518 (0.923-1.333) mmol/L; magnesium, 0.900 ± 0.158 (0.838-0.962) mmol/L; glucose, 2.97 ± 0.98 (2.58-3.36) mmol/L; lactate, 3.97 ± 1.09 (3.54-4.40) mmol/L; osmolality, 289.5 ± 6.9 (286.6-292.5) mOsm/kg; BOHB, 0.0937 ± 0.0472 (0.0750-0.1124) mmol/L; copper, 0.519 ± 0.269 (0.412-0.625) µmol/L; zinc, 1.95 ± 1.09 (1.52-2.38) µmol/L; selenium, 0.1035 ± 0.0276 (0.0923-0.1146) µmol/L; iron, 3.11 ± 1.40 (2.56-3.66) µmol/L; ferritin, 19.5 ± 10.3 (15.5-23.6) µg/L; transferrin, 0.0878 ± 0.0526 (0.0670-0.1086) g/L. Vitreous biochemistry was not significantly different between male and female participants. Vitreous biochemistry was significantly different between non-diabetic and diabetic participants. Vitreous biochemistry was significantly different from the vitreous substitute BSS Plus (Alcon, USA). The vitreous extracted from living humans was markedly different from the commonly reported reference values obtained from animal studies. The current data provide hitherto unavailable information about the biochemical composition of the living human vitreous. © 2016 Royal

  11. Human Dignity, Three Human Rights, and Pedagogy.

    Science.gov (United States)

    Vandenberg, Donald

    1986-01-01

    A general theory of value is outlined to show that moral agency is necessary to human dignity and that liberty, equality, and fraternity are necessary to moral agency. These ideals can be implemented in schools and human dignity can be at the core of the professional ethics of teaching. (MT)

  12. Human dignity, bioethics, and human rights.

    Science.gov (United States)

    Häyry, Matti; Takala, Tuija

    2005-09-01

    The authors analyse and assess the Universal Draft Declaration on Bioethics and Human Rights published by UNESCO. They argue that the Draft has two main weaknesses. It unnecessarily confines the scope of bioethics to life sciences and their practical applications. And it fails to spell out the intended role of human dignity in international ethical regulation.

  13. [Humanization in health care].

    Science.gov (United States)

    Oliveira, Beatriz Rosana Gonçalves de; Collet, Neusa; Viera, Cláudia Silveira

    2006-01-01

    This study aims to reflect on humanization in health care, recovering the history of understanding about mankind, the human and humanity, until humanization in humanity and health. We discuss the national humanization program in hospital care and reflect on this proposal and on the issue of humanization in Brazilian health care nowadays. Communication is indispensable to establish humanization, as well as technical and material conditions. Both users and health professionals need to be heard, building a network of dialogues to think and promote singular humanization actions. For this process to take effect, there is a need to involve the whole that makes up the health service. This group involves different professionals, such as managers, public policy makers, professional councils and education institutions.

  14. The Human Milk Oligosaccharide 2'-Fucosyllactose Quenches Campylobacter jejuni-Induced Inflammation in Human Epithelial Cells HEp-2 and HT-29 and in Mouse Intestinal Mucosa.

    Science.gov (United States)

    Yu, Zhuo-Teng; Nanthakumar, N Nanda; Newburg, David S

    2016-10-01

    Campylobacter jejuni causes diarrhea worldwide; young children are most susceptible. Binding of virulent C. jejuni to the intestinal mucosa is inhibited ex vivo by α1,2-fucosylated carbohydrate moieties, including human milk oligosaccharides (HMOSs). The simplest α1,2-fucosylated HMOS structure, 2'-fucosyllactose (2'-FL), can be predominant at ≤5 g/L milk. Although 2'-FL inhibits C. jejuni binding ex vivo and in vivo, the effects of 2'FL on the cell invasion central to C. jejuni pathogenesis have not been tested. Clinical isolates of C. jejuni infect humans, birds, and ferrets, limiting studies on its mammalian pathobiology. Human epithelial cells HEp-2 and HT-29 infected with the virulent C. jejuni strain 81-176 human isolate were treated with 5 g 2'-FL/L, and the degree of infection and inflammatory response was measured. Four-week-old male wild-type C57BL/6 mice were fed antibiotics to reduce their intestinal microbiota and were inoculated with C. jejuni strain 81-176. The sensitivity of the resulting acute transient enteric infection and immune response to inhibition by 2'-FL ingestion was tested. In HEp-2 and HT-29 cells, 2'-FL attenuated 80% of C. jejuni invasion (P jejuni colonization by 80%, weight loss by 5%, histologic features of intestinal inflammation by 50-70%, and induction of inflammatory signaling molecules of the acute-phase mucosal immune response by 50-60% (P jejuni clinical disease, 2'-FL inhibited pathogenesis and its sequelae. These data strongly support the hypothesis that 2'-FL represents a new class of oral agent for prevention, and potentially for treatment, of specific enteric infectious diseases. © 2016 American Society for Nutrition.

  15. Global Law: Humanism and Human Rights

    Directory of Open Access Journals (Sweden)

    Leilane Serratine Grubba

    2016-06-01

    Full Text Available This article discusses the ideal of human rights before globalizatórios inflows. It begins with a general statement about the legal globalization, comparing it with the Global Law in the political and economic implications. Later, it approaches the ideal predicted with transnationalism. Proposes a reflection on the present Human Rights. Also, rethinks the lines of the complex web of Global Law, its institutions and its actors, which circulate between the public and private plans. There is no sense in space maintenance of the ideal of human rights only in the state or territory in international treaties originally linked to the states.

  16. Human Resource Accounting System

    Science.gov (United States)

    Cerullo, Michael J.

    1974-01-01

    Main objectives of human resource accounting systems are to satisfy the informational demands made by investors and by operating managers. The paper's main concern is with the internal uses of a human asset system. (Author)

  17. Telling the Human Story.

    Science.gov (United States)

    Richardson, Miles

    1987-01-01

    Proposes that one of the fundamental human attributes is telling stories. Explores the debate on whether Neanderthals possessed language ability. Discusses the role of the "human story" in teaching anthropology. (DH)

  18. Human Use Index (Future