WorldWideScience

Sample records for human gingival crevicular

  1. Characterization of proteoglycan metabolites in human gingival crevicular fluid during orthodontic tooth movement.

    Science.gov (United States)

    Waddington, R J; Embery, G; Samuels, R H

    1994-05-01

    Previous studies have identified glycosaminoglycans in gingival crevicular fluid (GCF) associated with a variety of clinical conditions, notably those involving bone resorptive activity. GCF was here collected from around teeth undergoing active orthodontic movement. Proteoglycan metabolites were purified from GCF by anion-exchange chromatography using fast performance liquid chromatography. Sulphated glycosaminoglycan was associated with the most highly anionic protein fractions IV, V and VI, and biochemical analysis was restricted to these fractions. Analysis included glycosaminoglycan content by cellulose acetate electrophoresis, molecular size by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting and amino acid analyses. Fraction IV contained hyaluronan (18.7%) and chondroitin sulphate (10.9%), fraction V heparan sulphate (29.5%) and chondroitin sulphate (19.6%) and fraction VI chondroitin sulphate only (21.3%). SDS-PAGE revealed two Coomassie blue bands in fraction V of 72 and 60 kDa and two further bands in fraction VI of 71 and 56 kDa. These proteoglycans appeared resistant to digestion by chondroitinase ABC or heparinase III, although the glycosaminoglycan chains underwent degradation after protein-core removal. The molecular mass and amino acid composition of the chondroitin sulphate proteoglycan fractions showed a close similarity to those of human alveolar bone proteoglycan. The presence of heparan sulphate proteoglycan in GCF in association with orthodontic movement is in accord with previous reports. The findings support the view that proteoglycans in GCF are 'biomarkers', notably those associated with active resorption of alveolar bone.

  2. Levels of matrix metalloproteinase-7 and osteopontin in human gingival crevicular fluid during initial tooth movement

    Directory of Open Access Journals (Sweden)

    Dhaval Oswal

    2015-01-01

    Full Text Available Purpose: During orthodontic treatment, the early response of periodontal tissues to mechanical stress involves several metabolic changes that allow tooth movement. The purpose of this investigation was to evaluate osteopontin (OPN and matrix metalloproteinase (MMP-7 in the gingival crevicular fluid (GCF of human teeth exposed to orthodontic force. Materials and Methods: GCF samples were obtained from 15 healthy orthodontic patients (age, 12-22 years. In each patient, the left maxillary canine having the fixed orthodontic appliance was used as the test tooth, and its antagonist, with no appliance, was the control. Orthodontic force, 75 g was applied using a 16 × 22 beta titanium closing loop. The GCF sampling on the disto-buccal aspects of experimental and control tooth was performed at specific time interval with sterilized absorbent paper point. Processing was carried out with enzyme-linked immunosorbent assay to detect OPN and MMP-7 levels. Results: The peak level of OPN was seen after 1 h application of orthodontic force which was 1280.36 pg/ml ± 185.02. The peak level of MMP-7 was seen at 0 h which was 598.3 pg/ml ± 107.5. The levels of OPN after 1 h increased to 1280.36 pg/ml ± 185.02, and they decreased at 24 h to 1012.86 pg/ml ± 168.47 (P = 0.001. The levels of MMP-7 after 1 h decreased to 478 pg/ml ± 99.7 which increased at 24 h to 526.9 pg/ml ± 99.2. Conclusions: Orthodontic forces affect both OPN and MMP-7 protein levels on the compression side in a time-dependent fashion.

  3. RP-HPLC assay of doxycycline in human saliva and gingival crevicular fluid in patients with chronic periodontal disease.

    Science.gov (United States)

    Denić, Marko S; Sunarić, Slavica M; Kesić, Ljiljana G; Minić, Ivan Z; Obradović, Radmila R; Denić, Marija S; Petrović, Milica S

    2013-05-05

    A reversed-phased HPLC method with fluorescence detection was optimized and validated for determination of DOXY in human saliva and gingival crevicular fluid (GCF) with tetracycline as internal standard. Single step extraction with acetonitrile for both types of samples was performed. The separation was achieved at Zorbax Extend-C18 analytical column at 30°C. Mobile phase was consisted of an aqueous phase containing magnesium acetate, ammonium acetate, Na₂EDTA, triethyl-ammonium acetate buffered to pH 7.5 with ammonium hydroxide solution and acetonitrile. The volume ratio of the buffered water mixture of salts and acetonitrile was 86:14. Fluorescence detector was set at λex=380 nm and λem=520 nm. Under the optimized experimental conditions, good linearity was found in the range of 5.0-250.0 ng/mL for GCF with LOD of 1.63 ng/mL and LOQ of 4.93 ng/mL and 20.0-500.0 ng/mL for saliva with LOD of 6.36 ng/mL and LOQ of 19.28 ng/mL. This method was successfully applied for determination of DOXY in saliva and GCF obtained from patients with chronic periodontal disease. Copyright © 2013 Elsevier B.V. All rights reserved.

  4. Proviral HIV-1 DNA in gingival crevicular fluid of HIV-1-infected patients in various stages of HIV disease.

    Science.gov (United States)

    Maticic, M; Poljak, M; Kramar, B; Tomazic, J; Vidmar, L; Zakotnik, B; Skaleric, U

    2000-07-01

    The oral cavity is rarely reported to be a site of human immunodeficiency virus (HIV) transmission, despite detectable virus in saliva and relatively frequent prevalence of periodontal disease in HIV-infected persons yielding increased excretion of mononuclear-cell-enriched gingival fluid. To search for possible sources of HIV in saliva, and using the polymerase chain-reaction technique, we sought the presence and shedding patterns of proviral HIV-1 DNA in gingival crevicular fluid in a group of patients previously determined as HIV-1-seropositive. Periodontal status at the collection sites was monitored by several clinical parameters, including Plaque Index, Gingival Index, probing depth, and clinical attachment loss. Gingival crevicular fluid samples were collected by means of paper points. Proviral HIV-1 DNA was detected in the gingival fluid of 17 out of 35 HIV-1-infected patients. Its detection correlated significantly with higher plasma HIV-1 RNA viral load (p = 0.03) and not with peripheral blood CD4+ cell count, the presence of blood in gingival fluid, or oral lesions. There was a significant correlation between clinical attachment loss at the sites of fluid collection and plasma HIV-1 RNA viral load (p = 0.002), and borderline correlation between the latter and probing depth (p = 0.54) in the group of patients harboring proviral HIV-1 DNA in gingival crevicular fluid. The results of our study suggest that mononuclear cells present in gingival crevicular fluid and harboring proviral HIV-1 DNA could represent a potential source of HIV-1 in the presence or absence of local bleeding, especially in persons with advanced HIV infection and increased loss of clinical attachment.

  5. Assessment of periostin levels in serum and gingival crevicular fluid of patients with periodontal disease.

    Science.gov (United States)

    Balli, U; Keles, Z P; Avci, B; Guler, S; Cetinkaya, B O; Keles, G C

    2015-12-01

    Periostin, a secreted adhesion molecule essential for periodontal tissue integrity, is highly expressed in the periodontal ligament and plays a critical role in tooth and bone development. The purpose of this study was to investigate periostin levels in the gingival crevicular fluid and serum of patients with periodontal disease and compare them with those of healthy individuals. Eighty individuals (41 males and 39 females; age range: 25-48 years) were enrolled in the study. Individuals were divided into three groups following clinical and radiographic examinations: the periodontal-healthy group (n = 20), gingivitis group (n = 30) and chronic periodontitis group (n = 30). Gingival crevicular fluid and serum samples were collected and periostin levels were determined using the enzyme-linked immunosorbent assay. The total amount and concentration of periostin decreased in gingival crevicular fluid with the progression and severity of the disease from healthy controls to gingivitis and to chronic periodontitis groups and differed significantly (p 0.05). Periostin in gingival crevicular fluid negatively correlated with the gingival index in the periodontal disease groups, whereas it is inversely correlated with the clinical attachment level only in the periodontitis group (p periodontal disease, and negatively correlated with the clinical parameters. Within the limits of the study, the periostin level in gingival crevicular fluid can be considered a reliable marker in the evaluation of periodontal disease susceptibility and activity. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  6. Resistin in serum and gingival crevicular fluid as a marker of periodontal inflammation and its correlation with single-nucleotide polymorphism in human resistin gene at −420

    Directory of Open Access Journals (Sweden)

    Swati Pradeep Patel

    2013-01-01

    Full Text Available Aims: Resistin is an adipocytokine, which have been studied for its role in insulin resistance and recently in inflammation. The aim of the present study is to assess the concentration of resistin in serum and gingival crevicular fluid (GCF and to compare the levels between subjects with and without periodontitis and type 2 diabetes mellitus (T2DM and to further correlate the resistin levels with the single-nucleotide polymorphism (SNP at −420. Setting and Designs: A total of 96 subjects (48 males and 48 females were divided on the basis of gingival index (GI, probing pocket depth (PD, clinical attachment level (CAL and hemoglobin A 1c levels into healthy (group 1, n = 24, uncontrolled-diabetes related periodontitis (group 2, n = 24, controlled-diabetes related periodontitis (group 3, n = 24 and chronic periodontitis without T2DM (group 4, n = 24. Materials and Methods: The GCF and serum levels of resistin were quantified using the enzyme-linked immunosorbent assay and compared among the study groups. Further, the association of the resistin levels with periodontal inflammation and SNP at −420 was studied. Results and Conclusion: The resistin levels in GCF and serum from patients with periodontitis or diabetes mellitus related periodontitis (controlled or uncontrolled were higher than that of healthy subjects and correlated positively with GI. Further, subjects with GG genotype at −420 showed significantly higher GI, PD, CAL as compared with genotype group CC. Resistin was detected in all serum and GCF samples and was significantly higher in periodontitis. Further, GG genotype at −420 was associated significantly with periodontal inflammation and resistin levels.

  7. Salivary and gingival crevicular fluid histatin in periodontal health and disease

    OpenAIRE

    Bhadbhade, Smruti J.; Acharya, Anirudh B.; Thakur, Srinath L.

    2013-01-01

    Objectives: Histatin, with its anti bacterial, anti protease, and wound closure stimulating property might influence the pathogenesis of periodontal disease. This study assessed the presence of histatin in gingival crevicular fluid (GCF); the levels of salivary and GCF histatin in periodontal disease. Material and methods: It was a cross sectional study that included systemically healthy forty five subjects (22 males and 23 females) between the age group of 20 to 45 years. Based on Gingival I...

  8. Nitrite and Nitrate Levels of Gingival Crevicular Fluid and Saliva in Subjects with Gingivitis and Chronic Periodontitis

    Directory of Open Access Journals (Sweden)

    Ali Orkun Topcu

    2014-07-01

    Full Text Available Objectives: Nitrosative stress plays an essential role in the pathogenesis of periodontal disease. The aim of this study is to analyze the gingival crevicular fluid and saliva nitrite and nitrate levels in periodontally healthy and diseased sites. Material and Methods: A total of 60 individuals including, 20 chronic periodontitis and 20 gingivitis patients and 20 periodontally healthy controls participated in the present study. Probing depth, clinical attachment level, bleeding on probing, gingival index and plaque index were assessed, gingival crevicular fluid (GCF and saliva samples were obtained from the subjects, including 480 GCF samples and 60 unstimulated whole saliva samples. Nitrite and nitrate were analyzed by Griess reagent. Results: Total GCF nitrite levels were higher in gingivitis and periodontitis groups (1.07 [SD 0.62] nmol and 1.08 [SD 0.59] nmol than the control group (0.83 [SD 0.31] nmol (P 0.05. The difference in GCF nitrate level was not significant among the control, gingivitis and periodontitis groups (7.7 [SD 2.71] nmol, 7.51 [SD 4.16] nmol and 7.38 [SD 1.91] nmol. Saliva nitrite and nitrate levels did not differ significantly among three study groups. Saliva nitrate/nitrite ratios were higher in periodontitis and gingivitis groups than the control group. A gradual decrease in nitrate/nitrite ratio in GCF was detected with the presence of inflammation. Conclusions: It may be suggested that nitrite in gingival crevicular fluid is a better periodontal disease marker than nitrate and may be used as an early detection marker of periodontal inflammation, and that local nitrosative stress markers don’t show significant difference between the initial and advanced stages of periodontal disease.

  9. Gingival Crevicular Fluid Calprotectin, Osteocalcin and Cross-Linked N-Terminal Telopeptid Levels in Health and Different Periodontal Diseases

    Directory of Open Access Journals (Sweden)

    Sema Becerik

    2011-01-01

    Full Text Available Aim: The aim of the present study was to investigate gingival crevicular fluid (GCF calprotectin, osteocalcin and cross-linked N-terminal telopeptide (NTx levels in health along with different periodontal diseases.

  10. Gingival crevicular fluid MMP-8-concentrations in patients after acute myocardial infarction

    Directory of Open Access Journals (Sweden)

    Münzel Thomas

    2011-01-01

    Full Text Available Abstract Background The aim of this study was to determine the presence of matrix metalloproteinase-8 in the gingival crevicular fluid (GCF of patients after acute myocardial infarction (AMI. Methods A total of 48 GCF samples from 20 AMI patients, hospitalized at the Department of Cardiology and Angiology of the Johannes Gutenberg University Mainz, were investigated. Besides the myocardial infarction all patients suffered from chronic periodontal disease. Fifty-one GCF samples from 20 healthy age matched individuals with similar periodontal conditions served as controls. The dental examination included the assessment of oral hygiene, gingival inflammation, probing pocket depth, clinical attachment level and X-ray examination. The study was only carried out after the positive consent of the regional ethic commission. A quantitative assessment of aMMP-8 levels in the gingival crevicular fluid was performed with the help of the DentoAnalyzer (Dentognostics GmbH, Jena, Germany, utilising an immunological procedure. Results The aMMP-8 concentrations found in the gingival crevicular fluid of the AMI patients significantly differed (p = 0.001; mean value 30.33 ± 41.99 ng/ml aMMP-8 from the control group (mean value 10.0 ± 10.7 ng/ml aMMP-8. These findings suggest that periodontal inflammation in AMI patients might be associated with higher MMP-8-values compared to the healthy controls. Conclusions The acute myocardial infarction seems to influence the degree of periodontal inflammation, thus the measurement of the gingival crevicular fluid MMP8 levels seems to be a helpful biochemical test to obtain information about the severity of the periodontal disease.

  11. Effect of Periodontal Surgery on Osteoprotegerin Levels in Gingival Crevicular Fluid, Saliva, and Gingival Tissues of Chronic Periodontitis Patients

    Directory of Open Access Journals (Sweden)

    Sandy H. S. Hassan

    2015-01-01

    Full Text Available Objectives. This study was undertaken to investigate the OPG profiles in gingival crevicular fluid (GCF, saliva, and gingival tissues of chronic periodontitis (CP patients in response to open flap debridement (OFD. Subjects and Methods. The study included 30 subjects divided into 2 groups: 20 CP patients and 10 periodontally healthy subjects. Plaque index, gingival index, pocket depth, and clinical attachment level measurements were recorded for all subjects. GCF, salivary, and gingival samples were collected from all 30 subjects at baseline and 3 and 6 month after OFD from the 20 CP patients. GCF and salivary OPG levels were assessed by ELISA assay, while OPG expression in gingival tissues was examined by immunohistochemistry. Results. GCF, salivary and gingival OPG profiles were significantly higher in control subjects compared to CP patients at baseline (P<0.001. Within CP group, OPG levels in GCF, saliva, and gingival samples showed a significant increase at 3 and 6 months after OFD (P<0.001 compared to baseline. Although OPG values increased significantly in gingival samples and insignificantly in saliva after 3 months compared to 6 months, yet GCF levels were significantly decreased. Conclusions. OPG might be considered as a diagnostic and prognostic biomarker of periodontal bone destruction. This trial is registered with NCT02160613.

  12. Micro-Raman Spectroscopy for Monitoring Changes in Periodontal Ligaments and Gingival Crevicular Fluid

    Directory of Open Access Journals (Sweden)

    Carlo Camerlingo

    2014-11-01

    Full Text Available Micro-Raman Spectroscopy is an efficient method for analyzing biological specimens due to its sensitivity to subtle chemical and structural changes. The aim of this study was to use micro-Raman spectroscopy to analyze chemical and structural changes in periodontal ligament after orthodontic force application and in gingival crevicular fluid in presence of periodontal disease. The biopsy of periodontal ligament samples of premolars extracted for orthodontic reasons and the gingival crevicular fluid samples collected by using absorbent paper cones; were analyzed by micro-Raman spectroscopy. Changes of the secondary protein structure related to different times of orthodontic force application were reported; whereas an increase of carotene was revealed in patients affected by periodontal inflammation.

  13. Elevated levels of collagen cross-link residues in gingival tissues and crevicular fluid of teeth with periodontal disease.

    Science.gov (United States)

    Jepsen, Søren; Springer, Ingo N G; Buschmann, Andreas; Hedderich, Jürgen; Açil, Yahya

    2003-06-01

    Lysylpyridinoline (LP) and hydroxylysylpyridinoline (HP) are collagen cross-link residues. Lysylpyridinoline is present in most tissues, whereas LP is present mainly in mineralized tissue. Both are elevated in tissue with increased collagen resorption. The purpose of this investigation was to assess if the concentrations of LP and HP are elevated in gingiva and gingival crevicular fluid (GCF) of teeth with advanced periodontitis (AP). We investigated human gingival biopsies of healthy teeth (n = 19) and teeth with AP (n = 43) in 49 individuals. Samples of GCF from 54 teeth with AP were collected in seven patients and compared with samples from 11 patients with experimentally induced gingivitis. Levels of LP and HP were measured by HPLC and fluorescence detection. Gingival concentrations of HP but not LP around teeth with advanced periodontitis were significantly elevated compared with teeth with healthy periodontium. While significant amounts of HP and LP were measurable in the GCF of teeth with AP, no HP and LP was identified 3 months following non-surgical periodontal therapy of the teeth or in fluid from teeth subjected to experimentally induced gingivitis. Elevated concentrations of HP and LP in GCF may serve as indicators of ongoing destruction of periodontal tissues and alveolar bone in advanced periodontitis.

  14. An evaluation on elastase enzyme activity in gingival crevicular fluid in periodontitis

    Directory of Open Access Journals (Sweden)

    Qujeq D

    2003-08-01

    Full Text Available Statement of Problem: Changes in protein levels, host calls enzymes and inflammatory mediators in gingival"ncrevicular Fluid (GCF are considered as diagnostic indicators of Periodontitis."nPurpose: he aim of the present study was to measure the elastase enzyme activity in gingival crevicular Fluid"namong patients with periodontitis."nMaterial and Methods: In this study, 52 periodontitis patients (experimental group and 51 healthy subjects"nwithout any gingival inflammatio (control group were participated. Subjects of the periodontitis group"nshowed pockets of 4-5 mm depth without gingival enlargement and recession or pockets of 1-2 mm depth"nwith gingival recession. For enzyme activity measurement, lOOu,! of gingival fluid of each sample was mixed"nwith lOOu! of enzyme substrate on the tube. The mixture was incubated at 34°c for lh with a buffer solution"nof 1ml volume and absorbance was read at 410nm with spectrophotometer. The enzyme activity differences"nbetween two groups were analyzed by student t test."nResults: The elastase enzyme activity in gingival crevicular fluid in subjects with periodontium destruction"nand control subjects was 153±11.3 and 52.7±10.4 enzyme unit in ml per minute, respectively. The difference"nbetween groups was statistically significant (PO.05."nConclusion: Based on the findings of this study, the measurement of elastae enzyme activity could be a useful"nindication of tissue changes that may ultimately manifest clinically as periodontitis.

  15. Niveles de interleukina 17 en el fluido gingival crevicular de pacientes con peridontitis crónica progresiva Levels of interleukin-17 in gingival crevicular fluid (CGF of progressive chronic periodontitis patients

    Directory of Open Access Journals (Sweden)

    J. González Quesada

    2009-12-01

    Full Text Available Los biomarcadores de destrucción tisular presentes en el fluido gingival crevicular (GCF pueden ser de utilidad para identificar y predecir la progresión de la Enfermedad Periodontal y para ver la respuesta al tratamiento. La interleukina 17 (IL-17 es una citoquina producida por las células T CD4 y tiene un papel importante en la iniciación o mantenimiento de la respuesta pro-implantaria y recientemente se ha encontrado que estimula la reabsorción ósea osteoclástica. Esta función biológica es relevante en la etiopatogénesis de la periodontitis. El objetivo del presente estudio fue determinar la presencia de IL-17 en el fluido gingival crevicular de pacientes con periodontitis crónica progresiva. Material y método: En 14 sitios activos y 14 sitios inactivos, determinados de acuerdo con el método de tolerancia (Haffafie 1983 se tomó muestras de fluido gingival crevicular. La cantidad de IL-17 presente, se determinó con el Human IL-17 Inmunoassay Quantikine R&D INC. Mineapolis, USA. Los resultados se analizaron con el software Stata 7.0 y el Student's Test para muestras no pareadas. Resultados: La cantidad de IL-17 presente en el GCF fue significativamente mayor en los sitios activos, en relación con los sitios inactivos (p=0,0005. Conclusiones: Estos resultados sugieren la posibilidad de que la IL-17 podría ser un buen marcador de pérdida de inserción y destrucción de hueso alveolar.Biomarkers harvested from gingival crevicular fluid (GCF may be useful to identify and predict periodontal disease progression and to monitor the response to treatment. Interleukin-17 (IL-17 is a T-cell-derived cytokine that play a important role in the initiation or maintenance of the pro-inflamatory response and has recently been found to stimulate osteoclastic resorption. These biological functions are relevant to the aetiopathogenesis of periodontitis. Aim: The purpose of the present study was to determine the presence of IL-17 in gingival

  16. A novel method of sampling gingival crevicular fluid from a mouse model of periodontitis.

    Science.gov (United States)

    Matsuda, Shinji; Movila, Alexandru; Suzuki, Maiko; Kajiya, Mikihito; Wisitrasameewong, Wichaya; Kayal, Rayyan; Hirshfeld, Josefine; Al-Dharrab, Ayman; Savitri, Irma J; Mira, Abdulghani; Kurihara, Hidemi; Taubman, Martin A; Kawai, Toshihisa

    2016-11-01

    Using a mouse model of silk ligature-induced periodontal disease (PD), we report a novel method of sampling mouse gingival crevicular fluid (GCF) to evaluate the time-dependent secretion patterns of bone resorption-related cytokines. GCF is a serum transudate containing host-derived biomarkers which can represent cellular response in the periodontium. As such, human clinical evaluations of PD status rely on sampling this critical secretion. At the same time, a method of sampling GCF from mice is absent, hindering the translational value of mouse models of PD. Therefore, we herein report a novel method of sampling GCF from a mouse model of periodontitis, involving a series of easy steps. First, the original ligature used for induction of PD was removed, and a fresh ligature for sampling GCF was placed in the gingival crevice for 10min. Immediately afterwards, the volume of GCF collected in the sampling ligature was measured using a high precision weighing balance. The sampling ligature containing GCF was then immersed in a solution of PBS-Tween 20 and subjected to ELISA. This enabled us to monitor the volume of GCF and detect time-dependent changes in the expression of such cytokines as IL-1b, TNF-α, IL-6, RANKL, and OPG associated with the levels of alveolar bone loss, as reflected in GCF collected from a mouse model of PD. Therefore, this novel GCF sampling method can be used to measure various cytokines in GCF relative to the dynamic changes in periodontal bone loss induced in a mouse model of PD. Copyright © 2016. Published by Elsevier B.V.

  17. Protein carbonyl: An oxidative stress marker in gingival crevicular fluid in healthy, gingivitis, and chronic periodontitis subjects

    Directory of Open Access Journals (Sweden)

    Avani R Pradeep

    2013-01-01

    Full Text Available Background: A defined role for reactive oxygen species (ROS in the tissue destruction that characterizes periodontitis has been described. Protein carbonyl (PC is the most widely used biomarker for oxidative damage to proteins, and reflects cellular damage induced by multiple forms of ROS. The purpose of this study is to determine the presence of PC in gingival crevicular fluid (GCF in healthy, gingivitis, and chronic periodontitis (CP subjects and to find an association, if any. Materials and Methods: A total number of 75 subjects (38 males and 37 females were selected based on their clinical parameters into three groups: Group 1 (25 healthy subjects, Group 2 (25 gingivitis subjects, and Group 3 (25 CP subjects. GCF samples were collected to estimate the levels of PC. Results: The PC concentration in GCF was highest in subjects with CP as compared to gingivitis and healthy subjects and a significant association was observed between GCF PC levels and all periodontal parameters. Conclusion: There was an increase in PC levels in GCF as the disease process progressed from healthy to gingivitis and CP, suggesting a role for increased oxidative stress in CP.

  18. The Effect of Cigarette Smoking on Gingival Crevicular Fluid Flow.

    Science.gov (United States)

    1986-01-01

    Gregory, G.: An epidemiological study of chronic periodontal disease . Arch Oral Biol 10:553-566, 1965. 52. Shuler, R.L.: Effect of cigarette smoking on the... studies . Researchers have attempted to correlate plaque accumulation, calculus deposition, gingivitis, periodontal disease , and oral hygiene practices...in the etiology of periodontal disease . 15 In 1970 Alexander studied the relationship between tobacco smoking and periodontal health in 200 dental

  19. Deoxypyridinoline level in gingival crevicular fluid as alveolar bone loss biomarker in periodontal disease

    Directory of Open Access Journals (Sweden)

    Agustin Wulan Suci Dharmayanti

    2012-06-01

    Full Text Available Background: Periodontal diseases have high prevalence in Indonesia. They are caused by bacteria plaque that induced host response to release pro inflammatory mediator. Pro inflammatory mediators and bacteria product cause degradation of collagen fibers in periodontal tissue. Deoxypyridinoline is one of pyridinoline cross-link of collagen type I that can be used as biomarker in bone metabolic diseases, however, their contribution to detect alveolar bone loss in periodontal diseases remains unclear. Purpose: This study was to evaluate deoxypyridinoline level in gingival crevicular fluid as alveolar bone loss biomarker on periodontal disease. Methods: This study used 24 subjects with periodontal diseases and 6 healthy subjects. Dividing of periodontal disease was based on index periodontal. Gingival crevicular fluid was taken at mesial site of maxillary posterior tooth by paper point and deoxypyridinoline be measured by ELISA technique. Results: We found increasing of deoxypyridinoline level following of the severity of periodontal diseases. There was also significant difference between healthy subjects and periodontal diseases subjects (p<0.05. Conclusion: Deoxypyridinoline level in gingiva crevicular fluid can be used as alveolar bone loss biomarker in periodontal disease subjects.Latar belakang: Prevalensi penyakit periodontal di Indonesia cukup tinggi. Ini disebabkan oleh bakteri plak yang merangsang respon tubuh untuk mengeluarkan mediator keradangan. Mediator keradangan dan produk bakteri menyebabkan degradasi serat kolagen jaringan periodontal. Deoksipiridinolin merupakan salah satu ikatan piridinium dari kolagen tipe I yang dapat digunakan sebagai biomarker penyakit metabolisme tubuh. Akan tetapi, penggunaan deoksipiridinolin untuk mendeteksi kehilangan tulang alveolar pada penyakit periodontal masih belum jelas. Tujuan: Tujuan penelitian ini untuk mengetahui bahwa kadar deoksipiridinolin pada cairan krevikular gingival dapat digunakan

  20. Effect of two kinds of porcelain crown on AST, ALP, TNF-α, IL-8, GP-x and MDA levels in gingival crevicular fluid

    Institute of Scientific and Technical Information of China (English)

    Ya-Ling Wang; Zhi Cao; Shi-Xia Zhan

    2016-01-01

    Objective:To investigate the effect of two kinds of porcelain crown on AST, ALP, TNF-α, IL-8, GP-x and MDA levels in gingival crevicular fluid.Methods: A total of 80 patients with dental porcelain crowns at front teeth during February 2013 to February 2016 were randomly divided into cobalt-chromium alloy PFM group (n=40) and gold alloy PFM group (n=40). After 6 months, the amount of gingival crevicular fluid, GI, PD, AST, ALP, TNF-α, IL-8, GP-x and MDA levels in gingival crevicular fluid were recorded and analyzed.Results: There were no differences in amount of gingival crevicular fluid, GI and PD before treatment of the two groups (P>0.05). After treatment, the amount of gingival crevicular fluid, GI and PD of the two groups were significantly higher than before treatment (P0.05). After treatment, the AST, ALP, TNF-α, IL-8 and MDA levels in gingival crevicular fluid of the two groups were significantly higher than before treatment (P<0.05), but that of the gold alloy PFM group were significantly lower than cobalt-chromium alloy PFM group (P<0.05). After treatment, the GP-x level in gingival crevicular fluid of the two groups were significantly lower than before treatment (P<0.05), but that of the gold alloy PFM group were significantly higher than cobalt-chromium alloy PFM group (P<0.05).Conclusions:Gold alloy PFM can significantly reduce the AST, ALP, TNF-α, IL-8 and MDA levels in gingival crevicular fluid, improve the GP-x level in gingival crevicular fluid, shows better biocompatibility and clinical outcomes than cobalt-chromium alloy PFM.

  1. The effects of periodontal therapy on gingival crevicular fluid matrix metalloproteinase-8, interleukin-6 and prostaglandin E2 levels in patients with rheumatoid arthritis.

    Science.gov (United States)

    Kurgan, Ş; Fentoğlu, Ö; Önder, C; Serdar, M; Eser, F; Tatakis, D N; Günhan, M

    2016-10-01

    The aim of this study was to evaluate the effects of non-surgical periodontal therapy on gingival crevicular fluid levels of matrix metalloproteinase-8 (MMP-8), interleukin-6 (IL-6) and prostaglandin E2 (PGE2 ) in patients with rheumatoid arthritis (RA) with periodontal disease. Twenty-seven patients with gingivitis and periodontitis with RA, 26 patients with gingivitis and periodontitis that were systemically healthy and 13 periodontally and systemically healthy volunteers (control group) were included in this study. RA activity was assessed by disease activity score test. The clinical periodontal parameters, fasting venous blood and gingival crevicular fluid samples were obtained and gingival crevicular fluid MMP-8, IL-6 and PGE2 levels were evaluated at baseline and at 3 mo follow-up after non-surgical periodontal treatment. Gingival crevicular fluid MMP-8, PGE2 and IL-6 levels were higher in all groups than the control group. Following periodontal therapy, there were significant decreases in gingival crevicular fluid MMP-8, PGE2 and IL-6 levels from patients with RA with periodontitis (p periodontal treatment. Non-surgical periodontal therapy of patients with RA with periodontitis may provide beneficial effects on local inflammatory control via decreases in gingival crevicular fluid MMP-8, PGE2 and IL-6 levels. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  2. Gingival crevicular fluid in the diagnosis of periodontal and systemic diseases

    Directory of Open Access Journals (Sweden)

    Čakić Saša

    2009-01-01

    Full Text Available Gingival crevicular fluid (GCF can be found in the physiologic space (gingival sulcus, as well as in the pathological space (gingival pocket or periodontal pocket between the gums and teeth. In the first case it is a transudate, in the second an exudate. The constituents of GCF originate from serum, gingival tissues, and from both bacterial and host response cells present in the aforementioned spaces and the surrounding tissues. The collection and analysis of GCF are the noninvasive methods for the evaluation of host response in periodontal disease. These analyses mainly focus on inflammatory markers, such as prostaglandin E2, neutrophil elastase and β-glucuronidase, and on the marker of cellular necrosis - aspartat aminotransferase. Further, the analysis of inflammatory markers in the GCF may assist in defining how certain systemic diseases (e.g., diabetes mellitus can modify periodontal disease, and how peridontal disease can influence certain systemic disorders (atherosclerosis, preterm delivery, diabetes mellitus and some chronic respiratory diseases. Major factors which influence the results obtained from the analyses of GCF are not only the methods of these analyses, but the method of GCF collection as well. As saliva collection is less technique-sensitive than GCF collection, some constituents of saliva which originate from the GCF can be analyzed as more amenable to chairside utilization.

  3. Gingival crevicular blood: As a non-invasive screening tool for diabetes mellitus in dental clinics

    Directory of Open Access Journals (Sweden)

    Neema Shetty

    2013-01-01

    Full Text Available Background: A high number of patients with periodontitis may have undiagnosed diabetes. Self-monitoring devices provide a simple method for rapid monitoring of the glucose level in the blood by utilizing a blood sample from the finger, but this method requires a needle puncture to obtain blood. It is possible that gingival crevicular blood (GCB from routine periodontal probing may be a source of blood for glucose measurements. Aim: To establish whether GCB can be used as a non-invasive diagnostic aid in screening for diabetes mellitus during routine periodontal examination. Materials and Methods: The study involved 50 diabetics and 50 non-diabetics, with an age range of 26-66 years. Both diabetic and non-diabetic patients had moderate to severe gingivitis with at least one tooth in the maxillary anterior region showing bleeding upon probing. The Gingival Index and Oral Hygiene Index-Simplified were recorded. Blood oozing from the gingival sulcus/pocket following periodontal pocket probing was collected using a capillary tube and transferred to the test stick of a glucose self-monitoring device (Accu-Chek, Roche Diagnostic, Germany in patients with comparable gingival and oral hygiene status. This value was compared with the peripheral fingerstick blood glucose (PFBG value, which was obtained by pricking the finger tip at the same visit. Statistical analysis was performed using Pearson′s correlation coefficient. Result: There was no statistically significant difference between the gingival crevicular blood glucose (GCBG values and the PFBG values in both the diabetic (P = 0.129, NS and the non-diabetic (P = 0.503, NS groups. Karl Pearson′s product-moment correlation coefficient was calculated, which showed a positive correlation between the two measurements in the diabetic (r = 0.943 as well as the non-diabetic (r = 0.926 groups. Conclusion: The results suggest that GCB can be used as a non-invasive diagnostic aid in screening for diabetes

  4. Short-term effect of chewing gums containing probiotic Lactobacillus reuteri on the levels of inflammatory mediators in gingival crevicular fluid

    DEFF Research Database (Denmark)

    Twetman, Svante; Derawi, Bilal; Keller, Mette

    2009-01-01

    OBJECTIVE: To investigate the effect of a chewing gum containing probiotic bacteria on gingival inflammation and the levels of selected inflammatory mediators in gingival crevicular fluid (GCF). MATERIAL AND METHODS: Forty-two healthy adults with moderate levels of gingival inflammation entered...

  5. Effects of menstrual cycle on periodontal health and gingival crevicular fluid markers.

    Science.gov (United States)

    Becerik, Sema; Ozçaka, Ozgün; Nalbantsoy, Ayşe; Atilla, Gül; Celec, Peter; Behuliak, Michal; Emingil, Gülnur

    2010-05-01

    Fluctuations in sex steroid hormones, which are also noticeable through the menstrual cycle of women, may impact periodontal health. The aim of this study is to evaluate the effect of hormonal changes occurring in the menstrual cycle on gingival inflammation and the gingival crevicular fluid (GCF) levels of interleukin 6 (IL-6), prostaglandin E(2) (PGE(2)), tissue plasminogen activator (t-PA), and plasminogen activator inhibitor-2 (PAI-2). Twenty-five gingivitis patients and 25 periodontally healthy subjects having regular menstrual cycles were seen at menstruation (ME) (1 to 2 days of menstruation), ovulation (OV) (12 to 14 days), and premenstrual phases (PM) (22 to 24 days). GCF and saliva samples were collected and clinical parameters including plaque index and bleeding on probing were recorded at each menstrual phase. Salivary estrogen and progesterone levels were analyzed to determine exact menstrual cycle days. GCF levels of IL-6, PGE(2), t-PA, and PAI-2 were measured by enzyme-linked immunosorbent assay. The percentages of sites with bleeding on probing were significantly higher in ME (60.85 +/- 18.36) and OV (58.92 +/- 25.04) than in the PM (40.12 +/- 20.10) phase in the gingivitis group (P 0.05; repeated measures analysis of variance). GCF levels of IL-6 were significantly elevated in gingivitis patients compared to healthy subjects in all phases (P = 0.004, P = 0.041, and P = 0.046 for ME, OV, and PM, respectively; Mann-Whitney U test). GCF levels of IL-6, PGE(2), t-PA, and PAI-2 were unchanged in different menstrual phases in both groups (P >0.05; Friedman test). The present study suggests that changes in the sex steroid hormones during menstrual cycles might have a limited effect on the inflammatory status of gingiva, but GCF cytokine levels were not affected.

  6. Chlamydia pneumoniae clinical isolate from gingival crevicular fluid: a potential atherogenic strain

    Directory of Open Access Journals (Sweden)

    Simone eFilardo

    2015-11-01

    Full Text Available Chlamydia pneumoniae has been associated to atherosclerotic cardiovascular diseases. The aim of our study was to characterize, for the first time, a C. pneumoniae strain isolated from the gingival crevicular fluid of a patient with chronic periodontitis, described as a risk factor for cardiovascular diseases. C. pneumoniae isolate was characterized and compared to the respiratory AR-39 strain by VD4-ompA genotyping and by investigating the intracellular growth in epithelial and macrophage cell lines and its ability to induce macrophage-derived foam cells. Inflammatory cytokine levels were determined in the gingival crevicular fluid sample.C. pneumoniae isolate showed a 99% similarity with the AR-39 strain in the VD4-ompA gene sequence and shared a comparable growth kinetic in epithelial cells and macrophages, as evidenced by the infectious progeny and by the number of chlamydial genomic copies. C. pneumoniae isolate significantly increased the number of foam cells as compared to uninfected and LDL-treated macrophages (45% vs 6%, P = 0.0065 and to the AR-39 strain (45% vs 30%, P = 0.0065. Significantly increased levels of interleukin 1-β (2.1±0.3 pg/μL and interleukin 6 (0.6±0.08 pg/μL were found.Our results suggest that C. pneumoniae may harbor inside oral cavity and potentially be atherogenic, even though further studies will be needed to clarify the involvement of C. pneumoniae in chronic periodontitis as a risk factor for cardiovascular diseases.

  7. Gingival crevicular fluid adrenomedullin level in individuals with and without diabetes mellitus type 2.

    Science.gov (United States)

    Ertugrul, A S; Dikilitas, A; Sahin, H; Alpaslan, N Z; Bozoglan, A

    2013-06-01

    Adrenomedullin, an antimicrobial peptide, has biological applications in many tissues, but its main attribute is its ability to lower arterial pressure. The plasma adrenomedullin level is elevated in pathophysiological conditions such as arterial hypertension, acute coronary syndrome, renal diseases, diabetes mellitus and periodontal diseases. The aim of this study was to compare the amounts of adrenomedullin in the gingival crevicular fluid of periodontally healthy individuals, individuals with chronic periodontitis, periodontally healthy individuals with diabetes mellitus type 2 and individuals with chronic periodontitis and diabetes mellitus type 2. Eighty-four individuals were included in this study: 21 periodontally healthy individuals; 21 individuals with chronic periodontitis; 21 periodontally healthy individuals with diabetes mellitus type 2; and 21 individuals with chronic periodontitis and diabetes mellitus type 2. An ELISA was performed to measure the adrenomedullin levels in gingival crevicular fluid. Groups with diabetes mellitus type 2 (periodontally healthy individuals and individuals with chronic periodontitis) had significantly higher periodontal clinical indices than did nondiabetes mellitus groups (periodontally healthy individuals and individuals with chronic periodontitis). The group of individuals with chronic periodontitis and diabetes mellitus type 2 had a significantly higher total adrenomedullin level compared with the other groups. Also, a significantly higher total adrenomedullin level was found in diabetes mellitus type 2 groups (periodontally healthy individuals and individuals with chronic periodontitis) compared with nondiabetes mellitus groups (periodontally healthy individuals and individuals with chronic periodontitis). An increased adrenomedullin level was found in individuals with chronic periodontitis and also in individuals with diabetes mellitus. It is thought that the effect of diabetes mellitus on the pathogenesis of

  8. Assessment of gingival condition and cytokine content in gingival crevicular fluid after elastic fiber combined with flowable resin repair of pit and fissure caries

    Institute of Scientific and Technical Information of China (English)

    Zhao-Ran Fu

    2016-01-01

    Objective:To study the gingival condition and cytokine content in gingival crevicular fluid after elastic fiber combined with flowable resin repair of pit and fissure caries.Methods:A total of 68 patients with pit and fissure caries who received elastic fiber combined with flowable resin repair treatment in our hospital between May 2013 and August 2015 were selected as the research subjects and randomly divided into two groups, group A received elastic fiber combined with reinforced flowable resin material for repair and group B received elastic fiber combined with normal flowable resin material for repair. Four weeks after repair, gingival bleeding index was assessed, and gingival crevicular fluid was collected to determine the content of interleukins and protease molecules as well as the expression levels of autophagy genes.Results: Four weeks after treatment, the gingival bleeding index of group A was significantly lower than that of group B; IL-8, IL-17, IL-18, IL-21, IL-23, IL-35, MMP1, MMP2, MMP3 and MMP9 content as well asAtg3, Atg5, Atg7, Atg12 andLC-3II expression levels in gingival crevicular fluid of group A were significantly lower than those of control group while TIMP1 and TIMP2 content were significantly higher than those of group B. Conclusions: The gingival condition is more ideal after elastic fiber combined with reinforced flowable resin repair of pit and fissure caries, and reinforced flowable resin can alleviate the tissue injury mediated by interleukins, matrix metalloproteinases and autophagy.

  9. YKL-40 level in gingival crevicular fluid from patients with periodontitis and type 2 diabetes.

    Science.gov (United States)

    Kido, J; Bando, Y; Bando, M; Kajiura, Y; Hiroshima, Y; Inagaki, Y; Murata, H; Ikuta, T; Kido, R; Naruishi, K; Funaki, M; Nagata, T

    2015-07-01

    YKL-40 is a chitin-binding glycoprotein, the level of which increases in inflammatory diseases, diabetes mellitus (DM), cardiovascular diseases, and tumors. Gingival crevicular fluid (GCF) contains many proteins and markers of periodontitis. The purpose of this study was to investigate YKL-40 level in GCF from patients with periodontitis and DM and the association between YKL-40 level and chronic periodontitis (CP) or DM. The subjects were 121 patients with DM, CP, DM and periodontitis (DM-P), and healthy subjects (H). GCF was collected using paper strips after the sites for GCF collection were clinically evaluated for probing depth (PD), gingival index (GI), and bleeding on probing (BOP). YKL-40 in GCF was identified by Western blotting, and its level was determined by ELISA. YKL-40 was contained in GCF samples from H, DM, CP, and DM-P sites, and its levels (amount and concentration) in CP and DM-P were significantly higher than those in H and DM. GCF YKL-40 level significantly correlated with PD and GI, and its level in BOP-positive sites was significantly higher than that in BOP-negative ones. GCF YKL-40 level was elevated in periodontitis, but not DM. YKL-40 in GCF may be an inflammatory marker for periodontitis. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  10. Influence of dental filling material type on the concentration of interleukin 9 in the samples of gingival crevicular fluid

    OpenAIRE

    Stefanović Vladimir; Taso Ervin; Petković-Ćurčin Aleksandra; Đukić Mirjana; Gardašević Milka; Rakić Mia; Xavier Struillou; Jović Milena; Miller Karolina; Stanojević Ivan; Vojvodić Danilo

    2016-01-01

    Background/Aim. Several cytokines and lymphokines (IL1β, ENA78, IL6, TNFα, IL8 and S100A8) are expressed during dental pulp inflammation. Analysis of gingival crevicu-lar fluid (GCF) offers a non-invasive means of studying gen-eral host response in oral cavity. Although GCF levels of various mediators could reflect the state of inflammation both in dental pulp and gingiva adjacent to a tooth, GCF samples of those without significant gingivitis could be inter-preted as reflection of pulpal pro...

  11. Correlation between magnesium and alkaline phosphatase from gingival crevicular fluid periodontal diseases

    Directory of Open Access Journals (Sweden)

    Nila Kasuma

    2015-09-01

    Full Text Available Background: Magnesium is one of the alkaline phosphatase (ALP cofactor. The amount of magnesium contained infoods affect ALP activity. Increased ALP activity will indicate the level of inflammation in periodontal disease. Elevated inflammation in periodontal disease will change gingivitis to periodontitis, where there has been damage to the bone ssupporting the teeth, and an increasing number of gingival crevicular fluid (GCF. The content of GCF consists of enzymatic and non-enzymatic. Changes in the composition of GCF occurs when the inflammation gets worse. Purpose: This study was aimed to prove the correlation between magnesium and ALP from GCF on periodontal disease. Method: This research involved 60 Minangkabaunese people with 20 healthy samples, 20 mild gingivitis samples, and 20 mild periodontitis samples. GCF was collected by absorbing method. Then ALP level in GCF was measured by using ELISA technique. Magnesium level in Minangkabaunese food was tested by Food Frequency Questionnaire (FFQ.Univariate analysis was performed to describe each variable. To see a normal distribution, Kolmogorov Smirnof Test was used (p>0.05. Unpaired T-test and Pearson correlation test was used to see correlation between ALP and magnesium level in Minangkabaunese food. Result: There is a significant correlation between the levels of ALP and magnesium level in Minangkabaunese food with periodontal disease (p=0.005. ALP is higest on mild periodontitis (137.74±23.01 ng/dl. Magnesium level normal control group is highest (250.14±32.34 mg and in mild periodontitis is the lowest (110.83±21.04 mg. Corelation between ALP and magnesium level indicates strong correlation with negative direction (r=- 0.907. Conclusion: There is correlation between the levels of alkaline phosphatase and magnesium level on periodontal disease. Increasing inflamation rate will elevate the ALP level.

  12. Gingival crevicular fluid tissue/blood vessel-type plasminogen activator and plasminogen activator inhibitor-2 levels in patients with rheumatoid arthritis: effects of nonsurgical periodontal therapy.

    Science.gov (United States)

    Kurgan, Ş; Önder, C; Balcı, N; Fentoğlu, Ö; Eser, F; Balseven, M; Serdar, M A; Tatakis, D N; Günhan, M

    2017-06-01

    The aim of this study was to evaluate the effect of nonsurgical periodontal therapy on clinical parameters and gingival crevicular fluid levels of tissue/blood vessel-type plasminogen activator (t-PA) and plasminogen activator inhibitor-2 (PAI-2) in patients with periodontitis, with or without rheumatoid arthritis (RA). Fifteen patients with RA and chronic periodontitis (RA-P), 15 systemically healthy patients with chronic periodontitis (H-P) and 15 periodontally and systemically healthy volunteers (C) were included in the study. Plaque index, gingival index, probing pocket depth, clinical attachment level, bleeding on probing, gingival crevicular fluid t-PA and PAI-2 levels, erythrocyte sedimentation rate, serum C-reactive protein and disease activity score were evaluated at baseline and 3 mo after mechanical nonsurgical periodontal therapy. All periodontal clinical parameters were significantly higher in the RA-P and H-P groups compared with the C group (p periodontitis groups (p periodontitis and RA, nonsurgical periodontal therapy reduced the pretreatment gingival crevicular fluid t-PA levels, which were significantly correlated with gingival crevicular fluid PAI-2 levels. The significantly higher t-PA and PAI-2 gingival crevicular fluid levels in periodontal patients, regardless of systemic status, suggest that the plasminogen activating system plays a role in the disease process of periodontitis. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  13. LEVELS OF LL-37 ANTIMICROBIAL PEPTIDE IN THE GINGIVAL CREVICULAR FLUID OF YOUNG AND MIDDLE-AGED SUBJECTS WITH OR WITHOUT GINGIVITIS

    Directory of Open Access Journals (Sweden)

    Oya TÜRKOĞLU

    2017-01-01

    Full Text Available Purpose: LL-37 is an antimicrobial peptide which plays an important role in the innate immunity. The aim of this study was to investigate the LL-37 levels in the gingival crevicular fluid (GCF of middle-aged and young adults who have either gingivitis or healthy periodontal tissues. Materials and Methods: Forty middle-aged adults (20 healthy controls and 20 with gingivitis and 41 younger adults (20 healthy controls and 21 with gingivitis were included in the present study. Probing depth, clinical attachment level, plaque index, and papilla bleeding index were recorded. LL-37 levels in the GCF were analyzed by enzyme-linked immunosorbent assay. Results: No significant differences were observed in the GCF LL-37 levels between young healthy and middle-aged healthy subjects. Also, there were no significant differences in GCF LL-37 levels between young and middle-aged gingivitis subjects. However, gingivitis groups had significantly higher GCF LL-37 levels than healthy groups (p<0.001. Correlation analysis demonstrated no significant correlation between age and GCF LL-37 levels neither in healthy nor in gingivitis groups. Conclusion: The levels of LL-37 in GCF increase in the presence of gingival inflammation, however, this does not vary according to subjects being young or middle-aged.

  14. Identification of azurocidin as a potential periodontitis biomarker by a proteomic analysis of gingival crevicular fluid

    Directory of Open Access Journals (Sweden)

    Lee Jae-Mok

    2011-07-01

    Full Text Available Abstract Background The inflammatory disease periodontitis results in tooth loss and can even lead to diseases of the whole body if not treated. Gingival crevicular fluid (GCF reflects the condition of the gingiva and contains proteins transuded from serum or cells at inflamed sites. In this study, we aimed to discover potential protein biomarkers for periodontitis in GCF proteome using LC-MS/MS. Results We identified 305 proteins from GCF of healthy individuals and periodontitis patients collected using a sterile gel loading tip by ESI-MS/MS coupled to nano-LC. Among these proteins, about 45 proteins were differentially expressed in the GCF proteome of moderate periodontitis patients when compared to the healthy individuals. We first identified azurocidin in the GCF, but not the saliva, as an upregulated protein in the periodontitis patients and verified its increased expression during periodontitis by ELISA using the GCF of the classified periodontitis patients compared to the healthy individuals. In addition, we found that azurocidin inhibited the differentiation of bone marrow-derived macrophages to osteoclasts. Conclusions Our results show that GCF collection using a gel loading tip and subsequent LC-MS/MS analysis following 1D-PAGE proteomic separation are effective for the analysis of the GCF proteome. Our current results also suggest that azurocidin could be a potential biomarker candidate for the early detection of inflammatory periodontal destruction by gingivitis and some chronic periodontitis. Our data also suggest that azurocidin may have an inhibitory role in osteoclast differentiation and, thus, a protective role in alveolar bone loss during the early stages of periodontitis.

  15. Hepatocyte Growth Factor Levels in the Saliva and Gingival Crevicular Fluid in Smokers with Periodontitis

    Directory of Open Access Journals (Sweden)

    Sukumaran Anil

    2014-01-01

    Full Text Available Hepatocyte growth factor (HGF production by oral fibroblasts is enhanced by various molecules that are induced during inflammatory conditions including periodontitis. HGF plays an important role in the progression of periodontitis, by stimulating intense growth of epithelial cells and preventing regeneration of connective tissue attachments. Smokers have a greater risk factor in the pathogenesis and progression of periodontal disease. The objective of the study was to estimate the level of HGF in saliva and gingival crevicular fluid (GCF in smokers with periodontitis and to compare these levels with that of nonsmokers with periodontitis and healthy controls. The HGF levels were found to be significantly high in the saliva and GCF of smokers with periodontitis compared to both never-smokers with periodontitis and the healthy control group. The elevated levels of HGF in the saliva and GCF in the study population could explain the intrinsic mechanism triggering the severity of the periodontitis in smokers. Further studies are necessary to validate the current observations and to establish a sensitive marker to predict periodontal disease activity.

  16. Hepatocyte growth factor levels in the saliva and gingival crevicular fluid in smokers with periodontitis.

    Science.gov (United States)

    Anil, Sukumaran; Vellappally, Sajith; Preethanath, R S; Mokeem, Sameer A; AlMoharib, Hani S; Patil, Shankargouda; Chalisserry, Elna P; Al Kheraif, Abdulaziz A

    2014-01-01

    Hepatocyte growth factor (HGF) production by oral fibroblasts is enhanced by various molecules that are induced during inflammatory conditions including periodontitis. HGF plays an important role in the progression of periodontitis, by stimulating intense growth of epithelial cells and preventing regeneration of connective tissue attachments. Smokers have a greater risk factor in the pathogenesis and progression of periodontal disease. The objective of the study was to estimate the level of HGF in saliva and gingival crevicular fluid (GCF) in smokers with periodontitis and to compare these levels with that of nonsmokers with periodontitis and healthy controls. The HGF levels were found to be significantly high in the saliva and GCF of smokers with periodontitis compared to both never-smokers with periodontitis and the healthy control group. The elevated levels of HGF in the saliva and GCF in the study population could explain the intrinsic mechanism triggering the severity of the periodontitis in smokers. Further studies are necessary to validate the current observations and to establish a sensitive marker to predict periodontal disease activity.

  17. Estimation of gingival crevicular fluid aspartate aminotransferase levels in periodontal health and disease

    Directory of Open Access Journals (Sweden)

    Priti B Patil

    2011-01-01

    Full Text Available Background: Various enzymes have been assessed as biochemical markers and aspartate aminotransferase (AST is one such marker that has received considerable attention recently. Analysis of gingival crevicular fluid (GCF has been pursued as a means of identifying the sites undergoing active disease. A problem central to periodontology today is the inability to detect actively deteriorating sites and highly susceptible patients other than by longitudinal observations of attachment. Hence, AST levels from samples of GCF can be taken as an indication for active periodontal tissue destruction. Aim: To estimate the levels of AST in the GCF in periodontal health and disease. Materials and Methods: This study was an in vivo, case control, and clinico-biochemical assay. Eighty samples were selected which were divided into four groups of 20 patients each based on Russell′s Periodontal Index. Statistical analysis: The values obtained for AST level in the different groups were subjected to Student′s " t" test. Results: The mean of AST level showed an increase from Group I to Group IV. These values ran parallel with the values of clinical index, i.e. more severe the inflammation, higher the index score and higher was the AST level. Conclusions: It was concluded that as the severity of inflammation increases, there is a significant increase in the AST levels suggesting that there is a direct relationship between the AST levels in the GCF and periodontal destruction.

  18. PAR-2 expression in the gingival crevicular fluid reflects chronic periodontitis severity.

    Science.gov (United States)

    Fukushima, Henrique; Alves, Vanessa Tubero Euzebio; Carvalho, Verônica Franco de; Ambrósio, Lucas Macedo Batitucci; Eichler, Rosangela Aparecida Dos Santos; Carvalho, Maria Helena Catelli de; Saraiva, Luciana; Holzhausen, Marinella

    2017-01-26

    Recent studies investigating protease-activated receptor type 2 (PAR-2) suggest an association between the receptor and periodontal inflammation. It is known that gingipain, a bacterial protease secreted by the important periodontopathogen Porphyromonas gingivalis can activate PAR-2. Previous studies by our group found that PAR-2 is overexpressed in the gingival crevicular fluid (GCF) of patients with moderate chronic periodontitis (MP). The present study aimed at evaluating whether PAR-2 expression is associated with chronic periodontitis severity. GCF samples and clinical parameters, including plaque and bleeding on probing indices, probing pocket depth and clinical attachment level, were collected from the control group (n = 19) at baseline, and from MP patients (n = 19) and severe chronic periodontitis (SP) (n = 19) patients before and 6 weeks after periodontal non-surgical treatment. PAR-2 and gingipain messenger RNA (mRNA) in the GCF of 4 periodontal sites per patient were evaluated by Reverse Transcription Polymerase Chain Reaction (RT-qPCR). PAR-2 and gingipain expressions were greater in periodontitis patients than in control group patients. In addition, the SP group presented increased PAR-2 and gingipain mRNA levels, compared with the MP group. Furthermore, periodontal treatment significantly reduced (p periodontitis. In conclusion, PAR-2 is associated with chronic periodontitis severity and with gingipain levels in the periodontal pocket, thus suggesting that PAR-2 expression in the GCF reflects the severity of destruction during periodontal infection.

  19. Calcitonin gingival crevicular fluid levels and pain discomfort during early orthodontic tooth movement in young patients.

    Science.gov (United States)

    Alarcón, José Antonio; Linde, Dolores; Barbieri, Germán; Solano, Patricia; Caba, Octavio; Rios-Lugo, María Judith; Sanz, Mariano; Martin, Conchita

    2013-06-01

    To investigate the previously unreported presence of calcitonin (CT) levels in gingival crevicular fluid (GCF), its variations during initial orthodontic tooth movement in both tension and compression sites, and its possible association with the experienced dental pain. Fifteen children (mean age: 12.6 years) requiring orthodontic closure of the upper midline diastema were included. We collected GCF from the compression and tension sites of the upper right central incisor (experimental) and first bicuspid (control), before and after (1h, 24h, 7d, 15d) beginning of treatment. Calcitonin levels were determined by Western blot. Pain intensity was assessed using a visual analogue scale. Calcitonin levels were higher in the compression site versus the control site at 7d (p=0.014). Intragroup comparisons showed an increment of CT between 1h and 7d (680.81±1672.60pg/30s, p=0.010) in the compression site. No significant changes were found in the tension and control sites. Calcitonin levels and pain intensity were negatively associated during the period from 24h to 15d (r=-0.54, p=0.05). CT levels in the GCF significantly increased in the compression site after the short term after application of orthodontic forces. These changes were negatively associated with the perceived patient's dental pain during the period from 24h to 15d. Copyright © 2012 Elsevier Ltd. All rights reserved.

  20. Expression of Pentraxin 3 and Thrombospondin 1 in Gingival Crevicular Fluid during Wound Healing after Gingivectomy in Postorthodontic Patients.

    Science.gov (United States)

    Rauten, Anne Marie; Silosi, Isabela; Stratul, Stefan Ioan; Foia, Liliana; Camen, Adrian; Toma, Vasilica; Cioloca, Daniel; Surlin, Valeriu; Surlin, Petra; Bogdan, Maria

    2016-01-01

    Background. Wound healing is a tissue repair process after an injury, and two of its main components are inflammation and angiogenesis, in which course a cascade of mediators is involved. The aim of this research was to evaluate the involvement of Pentraxin 3 and Thrombospondin 1 in wound healing after periodontal surgery (gingivectomy) for gingival overgrowth during orthodontic treatment with or without magnification devices, by assessing their levels in GCF. Methods. From 19 patients with gingival overgrowth as a result of fixed orthodontic treatment, the overgrown gingiva was removed by gingivectomy, from one half of the mandibular arch without magnification and from the other under magnification. Pentraxin 3 and Thrombospondin 1 were determined from gingival crevicular fluid by ELISA tests. Results. Statistically significant differences (p gingivectomy. The dynamics of Pentraxin 3 and Thrombospondin 1 levels could suggest a reduced inflammation and a faster angiogenesis using microsurgery.

  1. Host mediators in gingival crevicular fluid: implications for the pathogenesis of periodontal disease.

    Science.gov (United States)

    Lamster, I B; Novak, M J

    1992-01-01

    During the past few years, a considerable number of studies have examined different aspects of the host response in gingival crevicular fluid (GCF), including the relationship of specific markers to the active phases of periodontal disease. Various indicators of the acute inflammatory response (the lysosomal enzymes beta-glucuronidase and collagenase, the cytoplasmic enzyme aspartate aminotransferase, and the arachidonic acid metabolite PGE2) have been shown to be associated with clinical attachment loss in chronic adult periodontitis in man and experimental periodontitis in animal models. In contrast, the relationship of indicators of the humoral immune response in GCF to active periodontal disease is equivocal. Furthermore, a number of indicators of the cellular immune response have been identified recently in GCF (i.e., Interleukin-1 alpha, IL-1 beta, tumor necrosis factor-alpha), but their relationship to active phases of periodontal disease have not been studied. The polymorphonuclear leukocyte (PMN) is the cellular hallmark of acute inflammation. Evidence from the GCF studies suggests that hyperreactivity of these cells plays a critical role in the active phases of some forms of periodontal disease. Metabolic activation of PMN can be associated with a number of potentially destructive reactions. The major effector mechanism for tissue destruction that can be specifically identified with the PMN is the synergistic effect of the release of PMN proteases and the generation of reactive oxygen metabolites by these cells. Priming of the PMN, where the PMN response is enhanced by agents that do not initiate the response, may be an important mechanism for PMN activation in the crevicular environment; for example, cytokines such as IL-1 beta and TNF-alpha, and lipopolysaccharides released from subgingival Gram-negative bacteria, can serve this function. The hypothesis proposed here argues that in addition to the severe forms of periodontal disease that have been

  2. Actividad de la lactato deshidrogenasa en fluído crevicular gingival y saliva en fumadores con periodontitis crónica Lactate dehidrogenase activity in gingival crevicular fluid and saliva of smoker with chronic periodontitis

    OpenAIRE

    E.R. Leyva Huerta; C. Esquivel Chirino; G. Marín González; M. Neblina Noriega; S. Olivares Tapia

    2009-01-01

    Existe un alto porcentaje de enfermedad periodontal atribuido al hábito del tabaco. La respuesta del organismo a esta enfermedad incluye la liberación de enzimas intracelulares relacionadas con la muerte y destrucción celular, como la Lactato Deshidrogenasa (LDH). Objetivo: Comparar los valores de LDH en fluido crevicular gingival (FCG) y saliva de pacientes fumadores y no fumadores con Periodontitis crónica (PC). Metodología: Participaron 15 pacientes hombres mayores de 30 años; 6 fumadores,...

  3. Niveles de interleukina 17 en el fluido gingival crevicular de pacientes con peridontitis crónica progresiva Levels of interleukin-17 in gingival crevicular fluid (CGF) of progressive chronic periodontitis patients

    OpenAIRE

    J. González Quesada; R. Jorquera Cortés; O. Jorquera Cortés; S. Rivera Álvarez

    2009-01-01

    Los biomarcadores de destrucción tisular presentes en el fluido gingival crevicular (GCF) pueden ser de utilidad para identificar y predecir la progresión de la Enfermedad Periodontal y para ver la respuesta al tratamiento. La interleukina 17 (IL-17) es una citoquina producida por las células T CD4 y tiene un papel importante en la iniciación o mantenimiento de la respuesta pro-implantaria y recientemente se ha encontrado que estimula la reabsorción ósea osteoclástica. Esta función biológica ...

  4. Evaluation of chemokines in gingival crevicular fluid in children with band and loop space maintainers: A clinico-biochemical study

    OpenAIRE

    Kumar, Naveen Kommineni; Reddy, Veera Kishore Kasa; Padakandla, Prathyusha; Togaru, Harshini; Kalagatla, Swathi; Vinay Chand M Reddy

    2016-01-01

    Background: Chemokines are pro-inflammatory cells that can be induced during an immune response to recruit cells of the immune system to a site of infection. Aim: This study was conducted to detect the presence of chemokines, macrophage inflammatory protein-1α (MIP-1α), and 1β (MIP-1β) and estimate their levels in gingival crevicular fluid (GCF) in children with band and loop space maintainers. Materials and Methods: MIP-1α and MIP-1β levels were estimated in GCF samples from twenty healthy c...

  5. PROSTAGLANDIN E2 LEVEL IN GINGIVAL CREVICULAR FLUID AND ITS RELATION TO THE PERIODONTAL POCKET DEPTH IN PATIENTS WITH PERIODONTITIS

    Institute of Scientific and Technical Information of China (English)

    周坚; 邹石莹; 赵戚; 赵玉霞

    1994-01-01

    Prostaglandin E2(PGE2)levels in gingival crevicular fluid (GCF)of 46 normal controls and 90 patients suf-fering from periodontitis with different periodontal pocket depths were measured by radioimmunoassay (RIA).The results demonstrated that PGE2 levels in the periodontal pockets are higher in patients with peri-odontitis.The PGE2 level rises as the periodontal pocket deepens,especially in casses where the periodontal pocket depth exceeds 6 mm.This study shows that PGE2 level is significantly related to the severity of bone destruc-tion in periodontitis.

  6. Relationship between cytokine levels in serum and gingival crevicular fluid (GCF) in pregnant women.

    Science.gov (United States)

    Fiorini, Tiago; Vianna, Priscila; Weidlich, Patricia; Musskopf, Marta Liliana; Moreira, Carlos Heitor Cunha; Chies, José Artur Bogo; Rösing, Cassiano Kuchenbecker; Oppermann, Rui Vicente; Susin, Cristiano

    2012-04-01

    Periodontal disease has been linked to systemic diseases/disorders and a low-grade systemic inflammatory status originated from periodontitis has been proposed as a possible explanation for this association. This study evaluates the relationship, early in pregnancy, between gingival crevicular fluid (GCF) and serum levels of a panel of cytokines that have been implicated in PTB and periodontal disease. One hundred pregnant women aged 18-35 years old with a gestational age up to 20 weeks were included (mean±SD gestational age:16.1±3.5 weeks). Four periodontal sites per subject were randomly selected for GCF collection. Serum and GCF levels of IL-1β, IL-6, IL-8, IL-10, IL-12p70 and TNF-α were analyzed using a cytometric bead array. Regression and correlation analyses were used to assess the relationship between serum and GCF cytokine levels. Participants had widespread periodontal inflammation but limited periodontal destruction. Cytokine levels were significantly higher in GCF than serum for all cytokines but IL-10. GCF levels had small but significant effect on serum levels for IL-10 (β=0.34±0.09, p<0.01), IL-12p70 (β=0.48±0.08, p<0.01) and TNF-α (β=0.29±0.09, p<0.01). Periodontal probing depth and bleeding on probing were significantly associated with GCF levels for IL-1β, IL-6 and IL-8; however, they had negligible effect on serum cytokine levels. Correlation between GCF and serum levels was non-significant, except for IL-12p70, which showed a significant but small correlation between the two sources (r=0.32, p=0.001). GCF cytokine levels were not strongly associated with serum cytokine levels in pregnant women with widespread periodontal inflammation but limited periodontal destruction. Copyright © 2012 Elsevier Ltd. All rights reserved.

  7. The Profile of Tooth and Gingival Crevicular Fluid Matrix Metalloproteinase-1 in Different Dental Diseases

    Directory of Open Access Journals (Sweden)

    Mutlak Shaimaa S

    2017-07-01

    Full Text Available Background: Pulpitis, apical periodontitis, and chronic periodontitis are the most common dental diseases and being the leading cause of tooth loss in adults. Aims: To unravel the changes and the interrelation of the biochemical and immunohistochemical levels of matrix metalloproteinase-1 (MMP-1 in the gingival crevicular fluid (GCF and teeth specimens of patients with different dental diseases. To test the influence of these changes on disease severity. Materials and methods: The GCF and tooth specimens were collected from 20 patients with chronic irreversible pulpitis (CIP, and similar number of patients with chronic periapical lesion (CPL, and chronic periodontitis (CP in addition to 20 healthy controls. Results: Statistically significant increase were found in the mean concentration of GCF-MMP1 of the patients within the CP and CIP groups over those of CIP and CPL groups (P<0.001. Highly significant elevation (P<0.001 in the means of cell with positive expression of the MMP-1 in all patient groups compared with the mean of the control group. The highest percentages of the MMP-1 expression (P=0.000 above the median values were seen in CPL (13.3% vs 86.7% followed by both CIP and CP groups (9.1% vs 90.9%. Using Receiver Operating Characteristic (ROC curve analysis, the GCF MMP-1 was found to be an effective test in CP group at reading ≥ 0.83 pg/ml and in CPL at cut off value of ≥ 2.24 ng/ml. Conclusion: The MMP1 plays a crucial role in the demolition of periodontal tissue and the GCF analyses can be used as noninvasive method to unravel these changes.

  8. Gingival crevicular fluid MMP-8 and -13 and TIMP-1 levels in patients with rheumatoid arthritis and inflammatory periodontal disease.

    Science.gov (United States)

    Biyikoğlu, Başak; Buduneli, Nurcan; Kardeşler, Levent; Aksu, Kenan; Pitkala, Marjut; Sorsa, Timo

    2009-08-01

    The purpose of this study was to compare gingival crevicular fluid (GCF) levels of matrix metalloproteinase (MMP)-8 and -13 and tissue inhibitor of MMP (TIMP)-1 in patients with rheumatoid arthritis (RA) and systemically healthy counterparts with inflammatory periodontal disease. Subjects (N = 74) were divided into five groups: 12 patients with RA and gingivitis; 13 patients with RA and periodontitis; 12 systemically healthy patients with gingivitis; 13 systemically healthy patients with periodontitis; and 24 periodontally and systemically healthy volunteers. Full-mouth clinical periodontal measurements were performed at six sites/tooth. GCF samples obtained from two sites in single-rooted teeth were analyzed by immunofluorometric assay and enzyme-linked immunosorbent assay. Data were assessed statistically by parametric tests. The total amounts of MMP-8 were lower in the healthy control group than in RA-gingivitis, RA-periodontitis, and healthy-periodontitis groups (P 0.05). Patients with RA and gingivitis or periodontitis exhibited levels of MMP-8 and -13 and TIMP-1 that were similar to systemically healthy counterparts (P >0.05). The coexistence of RA and periodontitis did not significantly affect the investigated parameters. GCF MMP-8 levels increased with periodontal inflammation. Despite the long-term usage of corticosteroids and non-steroidal anti-inflammatory drugs, similar GCF MMP-8 and -13 levels in patients with RA and systemically healthy counterparts suggest that RA may create a tendency to overproduce these enzymes.

  9. Gingival inflammation and interleukin-1 beta and tumor necrosis factor-alpha levels in gingival crevicular fluid during the menstrual cycle.

    Science.gov (United States)

    Baser, Ulku; Cekici, Ali; Tanrikulu-Kucuk, Sevda; Kantarci, Alpdogan; Ademoglu, Evin; Yalcin, Funda

    2009-12-01

    Hormonal changes during puberty, pregnancy, and menopause may impact periodontal tissues by altering the host response. There are only a few studies that examined gingival changes during the menstrual cycle. This longitudinal and prospective study aims to investigate clinical and laboratory markers of gingival inflammation in women at different phases during their menstrual cycles. Twenty-seven females were included in this study. Subjects were given oral hygiene instructions before the study, and their plaque index scores were recorded once a week for 2 months. The duration and regularity of the menstrual cycle were also checked at the same time. The gingival index and bleeding on probing (BOP) were recorded. Probing depths were measured to assess the periodontal condition of the subjects. Gingival crevicular fluid (GCF) was collected to analyze the levels of interleukin (IL)-1beta and tumor necrosis factor-alpha on the first menstruation day (MD), estimated ovulation day (OD), and estimated predominant progesterone secretion day (PgD). These exact menstrual cycle days were determined according to serum progesterone and estradiol levels. BOP and IL-1beta levels in GCF showed significant increases from the MD to PgD under optimal plaque control. Among the 12 subjects that had premenstrual symptoms, six subjects reported oral complaints during the premenstrual period, whereas apthous lesions were more frequent during the menstruation period. These results demonstrate that the fluctuation of sex steroid hormones impact gingival inflammation during menstruation.

  10. Actividad de la lactato deshidrogenasa en fluído crevicular gingival y saliva en fumadores con periodontitis crónica Lactate dehidrogenase activity in gingival crevicular fluid and saliva of smoker with chronic periodontitis

    Directory of Open Access Journals (Sweden)

    E.R. Leyva Huerta

    2009-04-01

    Full Text Available Existe un alto porcentaje de enfermedad periodontal atribuido al hábito del tabaco. La respuesta del organismo a esta enfermedad incluye la liberación de enzimas intracelulares relacionadas con la muerte y destrucción celular, como la Lactato Deshidrogenasa (LDH. Objetivo: Comparar los valores de LDH en fluido crevicular gingival (FCG y saliva de pacientes fumadores y no fumadores con Periodontitis crónica (PC. Metodología: Participaron 15 pacientes hombres mayores de 30 años; 6 fumadores, 6 no fumadores con PC y 3 sujetos control. Se recolectaron muestras de saliva total y de FCG de bolsas periodontales de = 6 mm. El volumen del FCG se determino pesando las puntas de papel antes y después del muestreo obteniendo los valores en gramos y convertidos a unidades de volumen (ml. La actividad de LDH se determino por medio de espectrofotometría. Los resultados se convirtieron a unidades de actividad enzimática expresados en mM. El volumen de FCG fue de 0,78 µL en sujetos sanos, en no fumadores con PC 1.12 y en fumadores con PC 1,32. Los valores de LDH correspondieron a; saliva de sujetos sanos 0,845, en no fumadores con PC 1,325 y en fumadores con PC 1,7895 mM. En FCG la actividad fue 0,4568 en sujetos sanos, en no fumadores con PC 0,987 y en fumadores con PC 1,2546 mM. Conclusiones: Las técnicas utilizadas permitieron determinar diferencia entre los valores de la actividad de LDH en ambos fluidos en fumadores y no fumadores con PC.The response of the body to periodontal disease includes the production of intra-cellular enzymes like Lactate Dehydrogenase (LDH which is released by damaged periodontal cells. The LDH can be found in the gingival crevicular fluid (GCF and saliva as a result of cellular death and damage. Objective: To compare LDH activity in GCF and saliva of smoker and non-smoker patients with Chronic Periodontitis. Methods: 15 male patients (30 to 50 years of age participated in this study; 6 smokers with Chronic Periodontitis

  11. Relation of soluble RANKL and osteoprotegerin levels in blood and gingival crevicular fluid to the degree of root resorption after orthodontic tooth movement.

    Science.gov (United States)

    Tyrovola, Joanna B; Perrea, Despoina; Halazonetis, Dimitrios J; Dontas, Ismene; Vlachos, Ioannis S; Makou, Margarita

    2010-06-01

    The aim of the present study was the determination of the levels of osteoprotegerin and soluble RANKL in blood serum and in gingival crevicular fluid relative to the degree of orthodontic root resorption in a rat model. Blood samples and gingival crevicular fluid were collected from fourteen 6-month-old male Wistar rats weighing 350-500 g. A 25-g closed orthodontic coil spring was inserted between each upper right first molar and the upper incisors. After 21 days of loading, both upper first molars (treated and control) were extracted and studied under microcomputed tomography scanning. Statistical analysis demonstrated a positive linear correlation between the initial concentration of RANKL in blood serum and the degree of root resorption. The ratio of the initial concentrations of osteoprotegerin to RANKL in blood serum proved to be an independent prognostic factor of the degree of root resorption. The initial concentration of RANKL in gingival crevicular fluid showed a negative correlation to the initial concentration of RANKL in blood serum and for a finite range of initial concentrations of osteoprotegerin in gingival crevicular fluid, the dental root seemed protected against extreme external root resorption. Finally, the concentration of osteoprotegerin in blood serum decreased significantly in cases of severe root resorption.

  12. Study of TNF-α, IL-1β and LPS Levels in the Gingival Crevicular Fluid of a Rat Model of Diabetes Mellitus and Periodontitis

    Directory of Open Access Journals (Sweden)

    Zhu-Ling Jiang

    2013-01-01

    Full Text Available OBJECTIVE: In this study, we sought to investigate the dynamic changes in the levels of TNF-α, IL-1β and LPS in the gingival crevicular fluid (GCF in a rat model of diabetes mellitus (DM and periodontitis (PD. Additionally, we evaluated alveolar bone loss and the histopathological response associated with experimental diabetes mellitus and experimental periodontitis.

  13. Influence of dental filling material type on the concentration of interleukin 9 in the samples of gingival crevicular fluid

    Directory of Open Access Journals (Sweden)

    Stefanović Vladimir

    2016-01-01

    Full Text Available Background/Aim. Several cytokines and lymphokines (IL1β, ENA78, IL6, TNFα, IL8 and S100A8 are expressed during dental pulp inflammation. Analysis of gingival crevicu-lar fluid (GCF offers a non-invasive means of studying gen-eral host response in oral cavity. Although GCF levels of various mediators could reflect the state of inflammation both in dental pulp and gingiva adjacent to a tooth, GCF samples of those without significant gingivitis could be inter-preted as reflection of pulpal process. The aim of this study was to investigate IL9 GCF values in patients with dental car-ies and to assess possible influence of various dental fillings materials on local IL9 production. Methods. The study group included 90 patients, aged 18–70, with inclusion and exclusion criteria in the prospective clinical study. Of the 6 types of material used for the restoration of prepared cavities, 3 were intended for temporary and 3 for definitive restora-tion. According to dental fillings weight, all the participants were divided into 3 groups: those with fillings lighter than 0.50 g, those with 0.50–1.00 g, and those with fillings heavier than 1.00 g. Samples were taken from gingival sulcus using the filter paper technique. Clinical parameters were deter-mined by bleeding index, plaque index (Silness-Lou, 0–3, gingival index (0–3, and gingival sulcus depth. Cytokine con-centrations were assessed using commercially available cy-tomix. Results. According to the weight of dental fillings, there was a clear decreament trend of IL9 values meaning that dental defects greater than 1.00 g of dental filling were associated with lower GCF IL9 concentration. The IL9 val-ues correlated with the degree of gingival index and depth of gingival sulcus, being higher with more advanced gingivitis and more pronounced anatomical changes in the tooth edge. Different filling materials exerted various local IL9 responses. Zink polycarbonate cement and amalgam fillings induced

  14. Effect of selective laser melting deposition cobalt chromium alloy porcelain crown restoration on related molecule contents in serum and gingival crevicular fluid

    Institute of Scientific and Technical Information of China (English)

    Yu-Hua Wei; Lei Yang

    2015-01-01

    Objective:To study the effect of selective laser melting deposition cobalt chromium alloy porcelain crown restoration on contents of inflammatory cytokines and adhesion molecules in serum and gingival tissue.Methods:80 cases of patients who received cobalt chromium alloy porcelain crown restoration in our hospital from May 2013 to August 2014 were enrolled and randomly divided into two groups. Observation group received selective laser melting deposition cobalt chromium alloy porcelain crown restoration and control group received casting cobalt chromium metal porcelain crown restoration. Then contents of inflammatory cytokines and adhesion molecules in serum and gingival crevicular fluid of both groups were detected.Results: (1) Inflammatory cytokines: compared with serum inflammatory cytokine contents of control group, serum NF-κB, IL-6, IL-8, IL-1β, TNF-α and NO contents of observation group trended to decrease; (2) Adhesion molecules in gingival crevicular fluid: compared with adhesion molecule contents in gingival crevicular fluid of control group, mRNA contents of CD11a, CD18, LFA-1, E-selectin and P-selectin in gingival crevicular fluid of observation group trended to decrease; (3) Adhesion molecules in serum: compared with adhesion molecule contents in serum of control group, sICAM-1 and sVCAM-1 contents in serum of observation group were lower.Conclusion: Selective laser melting deposition cobalt chromium alloy porcelain crown restoration is helpful to relieve inflammatory response of gingival tissue, with expression of decreased generation of inflammatory cytokines and adhesion molecules; it’s an ideal material for crown restoration.

  15. Expression of Pentraxin 3 and Thrombospondin 1 in Gingival Crevicular Fluid during Wound Healing after Gingivectomy in Postorthodontic Patients

    Directory of Open Access Journals (Sweden)

    Anne Marie Rauten

    2016-01-01

    Full Text Available Background. Wound healing is a tissue repair process after an injury, and two of its main components are inflammation and angiogenesis, in which course a cascade of mediators is involved. The aim of this research was to evaluate the involvement of Pentraxin 3 and Thrombospondin 1 in wound healing after periodontal surgery (gingivectomy for gingival overgrowth during orthodontic treatment with or without magnification devices, by assessing their levels in GCF. Methods. From 19 patients with gingival overgrowth as a result of fixed orthodontic treatment, the overgrown gingiva was removed by gingivectomy, from one half of the mandibular arch without magnification and from the other under magnification. Pentraxin 3 and Thrombospondin 1 were determined from gingival crevicular fluid by ELISA tests. Results. Statistically significant differences (p<0.05 and correlations between levels of the two biomarkers were analyzed. Statistically significant differences were established between levels of the two biomarkers at different time points, with significant positive correlation at the point of 24 hours. Conclusions. Within the limitations of this study, the results seem to sustain the involvement of Pentraxin 3 and Thrombospondin 1 in the processes of inflammation and angiogenesis in wound healing of patients with postorthodontic gingivectomy. The dynamics of Pentraxin 3 and Thrombospondin 1 levels could suggest a reduced inflammation and a faster angiogenesis using microsurgery.

  16. Evaluation of chemokines in gingival crevicular fluid in children with band and loop space maintainers: A clinico-biochemical study

    Science.gov (United States)

    Kumar, Naveen Kommineni; Reddy, Veera Kishore Kasa; Padakandla, Prathyusha; Togaru, Harshini; Kalagatla, Swathi; Reddy, Vinay Chand M.

    2016-01-01

    Background: Chemokines are pro-inflammatory cells that can be induced during an immune response to recruit cells of the immune system to a site of infection. Aim: This study was conducted to detect the presence of chemokines, macrophage inflammatory protein-1α (MIP-1α), and 1β (MIP-1β) and estimate their levels in gingival crevicular fluid (GCF) in children with band and loop space maintainers. Materials and Methods: MIP-1α and MIP-1β levels were estimated in GCF samples from twenty healthy children and twenty children with band and loop space maintainers. Periodontal status was evaluated by measuring gingival index, plaque index, and Russell's periodontal index. The GCF samples were quantified by ELISA, and the levels of MIP-1α and MIP-1β were determined. Results: The mean MIP-1α concentrations in healthy children and those with space maintainers were 395.75 pg/µl and 857.85 pg/µl, respectively, and MIP-1β was 342.55 pg/µl and 685.25 pg/µl, respectively. MIP-1α and MIP-1β levels in GCF from children with space maintainers were significantly higher than in the healthy group, and statistically significant difference existed between these two groups. Conclusion: MIP-1α and MIP-1β can be considered as novel biomarkers in the biological mechanism underlying the pathogenesis of gingival inflammation in children with space maintainers. PMID:27630491

  17. Evaluation of chemokines in gingival crevicular fluid in children with band and loop space maintainers: A clinico-biochemical study

    Directory of Open Access Journals (Sweden)

    Naveen Kommineni Kumar

    2016-01-01

    Full Text Available Background: Chemokines are pro-inflammatory cells that can be induced during an immune response to recruit cells of the immune system to a site of infection. Aim: This study was conducted to detect the presence of chemokines, macrophage inflammatory protein-1α (MIP-1α, and 1β (MIP-1β and estimate their levels in gingival crevicular fluid (GCF in children with band and loop space maintainers. Materials and Methods: MIP-1α and MIP-1β levels were estimated in GCF samples from twenty healthy children and twenty children with band and loop space maintainers. Periodontal status was evaluated by measuring gingival index, plaque index, and Russell′s periodontal index. The GCF samples were quantified by ELISA, and the levels of MIP-1α and MIP-1β were determined. Results: The mean MIP-1α concentrations in healthy children and those with space maintainers were 395.75 pg/µl and 857.85 pg/µl, respectively, and MIP-1β was 342.55 pg/µl and 685.25 pg/µl, respectively. MIP-1α and MIP-1β levels in GCF from children with space maintainers were significantly higher than in the healthy group, and statistically significant difference existed between these two groups. Conclusion: MIP-1α and MIP-1β can be considered as novel biomarkers in the biological mechanism underlying the pathogenesis of gingival inflammation in children with space maintainers.

  18. Effect of TNF-α Blockade in Gingival Crevicular Fluid on Periodontal Condition of Patients with Rheumatoid Arthritis.

    Science.gov (United States)

    Kadkhoda, Zeinab; Amirzargar, Aliakbar; Esmaili, Zahra; Vojdanian, Mahdi; Akbari, Solmaz

    2016-09-01

    Periodontitis and rheumatoid arthritis (RA) share a number of clinical and pathologic features, one of which is the presence of the tumor necrosis factor alpha (TNF-α)-induced bone resorption that is involved in the pathogenesis of both. To investigate the effect of TNF-α blockade on periodontal conditions in patients with active RA. The periodontal statuses of 36 patients (26 females, 10 males) diagnosed with active RA were evaluated both before and after anti-TNF-α therapy. Gingival index, bleeding on probing (BOP), probing pocket depth (PPD), oral hygiene index (OHI), and levels of TNF-α in gingival crevicular fluid (GCF) were measured at the baseline and 6 weeks after the treatment. Wilcoxon signed ranked test was used for statistical analyses. Based on OHI (p=0.860), the level of plaque control did not change during the study period, but there was a significant reduction in gingival inflammation based on the mean BOP (p=0.049) and GI (p=0.036) before and after 6 weeks of anti-TNF-α therapy. The mean PPD index did not significantly differ at the baseline and 6 weeks after treatment (p=0.126). Anti-TNF-α therapy might have a desirable effect on periodontal conditions and might reduce TNF-α level in GCF of patients with RA.

  19. Microfluidic Chip-based Nucleic Acid Testing using Gingival Crevicular Fluid as a New Technique for Detecting HIV-1 Infection

    Directory of Open Access Journals (Sweden)

    Alex Willyandre

    2013-05-01

    Full Text Available Transmission of HIV-1 infection by individuals in window period who are tested negative in conventional HIV-1 detection would pose the community with serious problems. Several diagnostic tools require specific labora-tory equipment, perfect timing of diagnosis, antibody to HIV-1, and invasive technique to get sample for examination, until high amount of time to process the sample as well as accessibility of remote areas. Many attempts have been made to solve those problems to come to a new detection technique. This review aims to give information about the current development technique for detection of HIV infection. Microfluidic Chip-based Nucleic Acid Testing is currently introduced for detection of HIV-1 infection. This review also cover the possible usage of gingival crevicular fluid as sample specimen that could be taken noninvasively from the individual.DOI: 10.14693/jdi.v18i2.63

  20. Effect of Non Surgical Periodontal Therapy on Gingival Crevicular Fluid and Serum Visfatin Concentration in Periodontal Health and Disease

    Directory of Open Access Journals (Sweden)

    N.M. Raghavendra

    2012-01-01

    Full Text Available Visfatin is a pleiotropic mediator which acts as growth factor, cytokine, enzyme involved in energy including nicotinamide adenine dinucleotide metabolism and has been recently demonstrated to exert several pro-inflammatory functions. The purpose of this study is to evaluate the Visfatin concentration in gingival crevicular fluid (GCF and serum in patients with chronic periodontitis, and to evaluate the effect of non-surgical periodontal therapy on the GCF and serum visfatin concentration. 30 subjects (age range: 25 to 52 years were selected and divided into two groups based on the gingival index, probing depth, periodontal attachment level, and radiologic parameters (bone loss: group 1 (15 subjects with healthy periodontium, group 2 (15 subjects with chronic periodontitis, while, Group 2 patients after 8 weeks of the treatment (scaling and root planning, SRP constituted group 3. GCF samples (by microcapillary pipettes and serum samples (by venipuncture were collected to estimate the levels of Visfatin using enzyme linked immunosorbent assay kit. The mean Visfatin concentration in GCF and serum was observed to be the highest in group 2 and lowest in group 1. While concentration in group 3 was similar to group 1. The concentration of Visfatin in GCF and serum decreased after SRP. The Visfatin concentration in GCF and serum found to be highest in chronic periodontitis group and decreases after treatment. Hence Visfatin values can be considered as an “inflammatory marker” can be explored in future as a potential therapeutic target in the treatment of periodontal disease.

  1. The use of 2 antibiotic regimens in aggressive periodontitis: comparison of changes in clinical parameters and gingival crevicular fluid biomarkers.

    Science.gov (United States)

    Machtei, Eli E; Younis, Mahmoud N

    2008-11-01

    To evaluate and compare the effect of nonsurgical periodontal therapy in conjunction with 2 different antibacterial agents on clinical and immunological parameters in aggressive periodontitis (AgP) patients. Thirty-one AgP patients underwent clinical examination that included plaque index (PI), gingival index (GI), probing pocket depth (PPD), and clinical attachment level (CAL). GCF (gingival crevicular fluid) samples were collected and assayed for PGE(2), IL-1beta, and TNFalpha. Nonsurgical periodontal therapy was performed. The patients received doxycycline (doxy group) or metronidazole plus amoxicillin (am-met group). After completion of the mechanical phase of therapy, patients were seen biweekly for oral hygiene reinforcement. After 12 weeks, clinical measurements and GCF sampling were repeated. The combined mechanical and antimicrobial therapy resulted in overall improvement in all clinical parameters; mean PPD reduction of 0.74 +/- 0.1 mm and mean CAL gain 0.86 +/- 0.1 mm were both statistically significant (P = .0001). Marked reduction was observed for PGE2 and IL-1beta, but not TNFalpha. There was no significant difference between the 2 groups at baseline or final examination, except for PGE(2) at final examination: It was significantly lower for the doxy group (83.61 +/- 5.8 ng) than for the am-met group (124.08 +/- 15.8 ng), P = .0202. Both treatment protocols resulted in significant improvements in the clinical and immunological parameters. When combined mechanical and antimicrobial protocol is desired and microbial cultures are not available, the use of both antibiotic regimens is recommended; the treatment of choice should be based on compliance, allergies, and potential side effects.

  2. Impact of scaling and root planing on C-reactive protein levels in gingival crevicular fluid and serum in chronic periodontitis patients with or without diabetes mellitus.

    Science.gov (United States)

    Mohan, Mahendra; Jhingran, Rajesh; Bains, Vivek Kumar; Gupta, Vivek; Madan, Rohit; Rizvi, Iram; Mani, Kanchan

    2014-08-01

    The present study was conducted to evaluate the impact of scaling and root planing (SRP) on the C-reactive protein (CRP) levels of gingival crevicular fluid (GCF) and serum in chronic periodontitis patients with type 2 diabetes mellitus (T2DM-CP) or without type 2 diabetes mellitus (NDM-CP). Forty-eight human participants were divided into two groups: an experimental (T2DM-CP) group (group I, n=24) comprising chronic periodontitis patients with random blood sugar ≥200 mg/dL and type 2 diabetes mellitus, and control (NDM-CP) group (group II, n=24) of those with chronic periodontitis and random blood sugar periodontal therapy (NSPT) including complete SRP and subgingival debridement. Periodontal health parameters, plaque index (PI), gingival index (GI), probing pocket depth (PPD), clinical attachment level (CAL), GCF volume (GCF vol), GCF-CRP, random blood glucose (RBS), glycated hemoglobin, and systemic inflammatory markers, serum CRP, total leukocyte count (TLC), neutrophil count (Neutr) and lymphocyte count (Lymph), were evaluated at baseline, 1 month, and 3 months after SRP. NSPT resulted in statistically significant improvement in periodontal health parameters (PI, GI, PPD, CAL, GCF vol), CRP levels in serum as well as GCF of both groups I and II. The mean improvement in periodontal health parameters (PI, GI, PPD, CAL, GCF vol), CRP levels in serum and GCF was greater in group I than group II after NSPT. There was nonsignificant increase in GCF-CRP, TLC, Lymph, and RBS, and a significant increase in Neutr and Serum CRP in group II at 1 month. The Serum CRP level of 20 out of 24 group II patients had also increased at 1 month. The CRP levels in both GCF and serum were higher in T2DM-CP patients than in NDM-CP patients. Although there was a significant improvement in both the groups, greater improvement was observed in both GCF and serum samples of T2DM-CP patients.

  3. Vitamin D-Binding Protein Levels in Plasma and Gingival Crevicular Fluid of Patients with Generalized Aggressive Periodontitis

    Directory of Open Access Journals (Sweden)

    Xin Zhang

    2014-01-01

    Full Text Available Vitamin D-binding protein (DBP is the main transport protein of vitamin D and plays an important role in the immune system and host defenses. The purpose of this study was to measure DBP levels in plasma and gingival crevicular fluid (GCF of patients with generalized aggressive periodontitis (GAgP, in comparison to healthy controls, with the goal of elucidating the relationship between DBP and GAgP. Fifty-nine GAgP patients and 58 healthy controls were recruited for the study; clinical parameters of probing depths (PD, bleeding index, and attachment loss (AL were recorded. DBP levels were measured by enzyme-linked immunosorbent assay. From the results, GAgP patients had higher plasma DBP concentrations (P<0.001 but lower GCF DBP concentrations (P<0.001 than healthy controls. In GAgP group, after controlling the potential confounders of age, gender, smoking status, and BMI index, GCF DBP concentrations correlated negatively with PD (P<0.001 and AL (P=0.009. Within the limits of the study, we concluded that decreased GCF DBP level and increased plasma DBP level are associated with periodontitis.

  4. Correlation of Hepatitis C Antibody Levels in Gingival Crevicular Fluid and Saliva of Hepatitis C Seropositive Hemodialysis Patients

    Directory of Open Access Journals (Sweden)

    Gökhan Açıkgöz

    2009-01-01

    Full Text Available Search for hepatitis C virus (HCV in body fluids other than blood is important when assessing possible nonparenteral routes of viral transmission. However, the role of oral fluids in HCV transmission remains controversial. Our aim was to compare the prevalence of HCV antibody (HCV Ab levels in saliva, and gingival crevicular fluid (GCF of HCV seropositive hemodialysis patients. Serum, saliva and GCF samples were collected from thirty-nine patients. Samples were analyzed for HCV Ab using the Ortho HCV 3.0 SAVe enzyme-linked immunosorbent assay (ELISA. HCH Ab levels in saliva and GCF of all HCV-seropositive patients were statistically compared. Reported here are the results of the study designed to determine the correlation between HCV-RNA positivity in serum and the detection of antibodies in GCF and saliva. One hundred percent (100% of the 39 patients have antibodies to HCV in their serum, 15.4% have antibodies to HCV in GCF, and saliva found out. HCV Ab seropositivity in GCF and saliva was significantly correlated (kappa = 0.462; <.001. This study supports the concept that GCF may be a significant source of HCV in saliva.

  5. Quantitative Gingival Crevicular Fluid Proteome in Health and Periodontal Disease Using Stable-Isotope Chemistries and Mass Spectrometry

    Science.gov (United States)

    Carneiro, Leandro G.; Nouh, Hesham; Salih, Erdjan

    2014-01-01

    Aim Application of quantitative stable-isotope-labeling chemistries and mass spectrometry (MS) to determine alterations in gingival crevicular fluid (GCF) proteome in periodontal disease. Materials and Methods Quantitative proteome of GCF from 40 healthy individuals versus 40 patients with periodontal disease was established using 320 GCF samples and stable-isotope-labeling reagents, ICAT and mTRAQ, with MS technology and validated by enzyme-linked immunosorbent methods. Results We have identified 238 distinct proteins of which 180 were quantified in GCF of both healthy and periodontal patients with additional 26 and 32 distinct proteins that were found only in GCF of healthy or periodontal patients. In addition, 42 pathogenic bacterial proteins and 11 yeast proteins were quantified. The data highlighted a series of proteins not quantified previously by large-scale MS approaches in GCF with relevance to periodontal disease, such as host derived Ig alpha-2 chain C, Kallikrein-4, S100-A9, transmembrane proteinase 13, peptidase S1 domain, several collagen types and pathogenic bacterial proteins e.g., formamidase, leucine amidopeptidase and virulence factor OMP85. Conclusions The innovative analytical approaches provided detailed novel changes in both host and microbial derived GCF proteomes of periodontal patients. The study defined 50 host and 16 pathogenic bacterial proteins significantly elevated in periodontal disease most of which were novel with significant potential for application in the clinical arena of periodontal disease. PMID:24738839

  6. The Gingival Crevicular Fluid as a Source of Biomarkers to Enhance Efficiency of Orthodontic and Functional Treatment of Growing Patients.

    Science.gov (United States)

    de Aguiar, Mariana Caires Sobral; Perinetti, Giuseppe; Capelli, Jonas

    2017-01-01

    Gingival crevicular fluid (GCF) is a biological exudate and quantification of its constituents is a current method to identify specific biomarkers with reasonable sensitivity for several biological events. Studies are being performed to evaluate whether the GCF biomarkers in growing subjects reflect both the stages of individual skeletal maturation and the local tissue remodeling triggered by orthodontic force. Present evidence is still little regarding whether and which GCF biomarkers are correlated with the growth phase (mainly pubertal growth spurt), while huge investigations have been reported on several GCF biomarkers (for inflammation, tissue damage, bone deposition and resorption, and other biological processes) in relation to the orthodontic tooth movement. In spite of these investigations, the clinical applicability of the method is still limited with further data needed to reach a full diagnostic utility of specific GCF biomarkers in orthodontics. Future studies are warranted to elucidate the role of main GCF biomarkers and how they can be used to enhance functional treatment, optimize orthodontic force intensity, or prevent major tissue damage consequent to orthodontic treatment.

  7. The Gingival Crevicular Fluid as a Source of Biomarkers to Enhance Efficiency of Orthodontic and Functional Treatment of Growing Patients

    Science.gov (United States)

    Capelli, Jonas

    2017-01-01

    Gingival crevicular fluid (GCF) is a biological exudate and quantification of its constituents is a current method to identify specific biomarkers with reasonable sensitivity for several biological events. Studies are being performed to evaluate whether the GCF biomarkers in growing subjects reflect both the stages of individual skeletal maturation and the local tissue remodeling triggered by orthodontic force. Present evidence is still little regarding whether and which GCF biomarkers are correlated with the growth phase (mainly pubertal growth spurt), while huge investigations have been reported on several GCF biomarkers (for inflammation, tissue damage, bone deposition and resorption, and other biological processes) in relation to the orthodontic tooth movement. In spite of these investigations, the clinical applicability of the method is still limited with further data needed to reach a full diagnostic utility of specific GCF biomarkers in orthodontics. Future studies are warranted to elucidate the role of main GCF biomarkers and how they can be used to enhance functional treatment, optimize orthodontic force intensity, or prevent major tissue damage consequent to orthodontic treatment. PMID:28232938

  8. Periodontal therapy in chronic periodontitis lowers gingival crevicular fluid interleukin-1beta and DAS28 in rheumatoid arthritis patients.

    Science.gov (United States)

    Bıyıkoğlu, Başak; Buduneli, Nurcan; Aksu, Kenan; Nalbantsoy, Ayşe; Lappin, David F; Evrenosoğlu, Evren; Kinane, Denis F

    2013-10-01

    To evaluate clinical outcomes and effects of non-surgical periodontal therapy on serum, gingival crevicular fluid (GCF) interleukin-1beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) levels in chronic periodontitis patients with/without rheumatoid arthritis (RA), fifteen RA patients with chronic periodontitis (RA-P) and 15 systemically healthy non-RA chronic periodontitis patients (H-P) were recruited. Clinical periodontal recordings, GCF, and blood samples were obtained at baseline, 1, 3, and 6 months after periodontal treatment. GCF, serum IL-1β, TNF-α levels were analyzed by ELISA. Disease activity score 28 (DAS28) was used to assess RA clinical morbidity. Study groups were compared by Mann-Whitney U test. Wilcoxon test was used to compare the data at baseline, 1, 3, and 6 months after periodontal therapy within the same group. DAS28 decreased significantly after periodontal therapy in RA-P group (p periodontal therapy. GCF IL-1β amounts decreased significantly in both groups following treatment (p periodontal indices (p periodontal treatment suggest that periodontal therapy synergizes with systemic RA therapy to improve RA status.

  9. Comparative study of substance P and neurokinin A in gingival crevicular fluid of healthy and painful carious permanent teeth

    Science.gov (United States)

    Heidari, Alireza; Shahrabi, Mahdi; Rokouei, Mehrak; Amirzargar, Aliakbar; Rahbar, Pegah

    2017-01-01

    Background: It is shown that neuropeptides can be transported from pulp chamber to periodontal ligament through apical foramen and accessory canals. Therefore, clinical pulpal pain leads to expression of preinflammatory neuropeptides such as substance P (SP) and neurokinin A (NKA) in gingival crevicular fluid (GCF). This study aimed to evaluate levels of SP and NKA in GCF of carious and healthy permanent teeth, comparatively. Materials and Methods: This cross-sectional study was performed on twenty children referred to Department of Pediatric Dentistry, Tehran University of Medical Sciences, who had a painful permanent first molar. Sampling was done by sterile paper cone from GCF of the mentioned teeth and the intact tooth of the other side of the jaw in the same patient. Values of SP and NKA were measured by ELISA test. Results: The mean concentration of SP in GCF of painful carious and healthy teeth was 2.65 ± 0.56 and 1.83 ± 0.65 pcgr/ml, respectively. This value was 2.29 ± 0.29 and 1.61 ± 0.35 pcgr/ml for NKA concentration in carious and healthy teeth as well. Conclusion: Significant higher levels of both SP and NKA in GCF of painful carious teeth were observed, which is in line with previous studies’ findings.

  10. Comparative study of substance P and neurokinin A in gingival crevicular fluid of healthy and painful carious permanent teeth.

    Science.gov (United States)

    Heidari, Alireza; Shahrabi, Mahdi; Rokouei, Mehrak; Amirzargar, Aliakbar; Rahbar, Pegah

    2017-01-01

    It is shown that neuropeptides can be transported from pulp chamber to periodontal ligament through apical foramen and accessory canals. Therefore, clinical pulpal pain leads to expression of preinflammatory neuropeptides such as substance P (SP) and neurokinin A (NKA) in gingival crevicular fluid (GCF). This study aimed to evaluate levels of SP and NKA in GCF of carious and healthy permanent teeth, comparatively. This cross-sectional study was performed on twenty children referred to Department of Pediatric Dentistry, Tehran University of Medical Sciences, who had a painful permanent first molar. Sampling was done by sterile paper cone from GCF of the mentioned teeth and the intact tooth of the other side of the jaw in the same patient. Values of SP and NKA were measured by ELISA test. The mean concentration of SP in GCF of painful carious and healthy teeth was 2.65 ± 0.56 and 1.83 ± 0.65 pcgr/ml, respectively. This value was 2.29 ± 0.29 and 1.61 ± 0.35 pcgr/ml for NKA concentration in carious and healthy teeth as well. Significant higher levels of both SP and NKA in GCF of painful carious teeth were observed, which is in line with previous studies' findings.

  11. Effect of periodontal therapy on metabolic control and levels of IL-6 in the gingival crevicular fluid in type 2 diabetes mellitus.

    Science.gov (United States)

    Camargo, Gabriela Alessandra da Cruz Galhardo; Lima, Meyriane de Andrade; Fortes, Tânia Vieira; de Souza, Cristiane Salgado; de Jesus, Amelia Maria; de Almeida, Roque Pacheco

    2013-01-01

    The aim of this study was to compare the efficacy of metabolic control and levels of interleukin 6 (IL-6) in gingival crevicular fluid after periodontal therapy in type 2 diabetes mellitus (DM) and nondiabetic (NDM) patients. This study was performed in 20 subjects (10 type 2 DM and 10 NDM patients with generalized chronic periodontal disease. Both groups were recorded for clinical parameters (plaque index (PI), gingival index (GI), probing depth (PD), gingival recession (GR) and clinical attachment level (CAL)), metabolic control (fasting glucose levels, glycated a-hemoglobin (HbA1c), low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglycerides (TR)), and IL-6 levels at baseline and 3 months after periodontal treatment. DM and NDM patients revealed significant statistical reductions for clinical parameters (P periodontal therapy. However, TRG levels increased after 3 months, which suggest more confirmatory studies to investigate if these results will be repeated in other studies.

  12. Levels of interleukin-1β in gingival crevicular fluid in patients with coronary heart disease and its relationship to periodontal status

    Science.gov (United States)

    Lenggogeny, Putri; Masulili, Sri Lelyati C.; Tadjoedin, Fatimah M.; Radi, Basuni

    2017-02-01

    Periodontitis is a risk factor for coronary heart disease (CHD). Both diseases are an inflammatory diseases and have the same potential pathogenic mechanisms. Interleukin-1β as a pro-inflammatory main cytokine, can be found in this both diseases. Gingival crevicular fluid (GCF) derived from the serum of gingival sulcus, affected by inflammatory mechanism and the amount of this fluid will increase in that situation. Objective: To analyze the relationship of interleukin-1β levels in gingival crevicular fluid (GCF) of CHD and non-CHD patients with periodontal status. Methods: Oral clinical examination (plaque index, bleeding on probing, pocket depth and clinical attachment loss) for 35 subjects with CHD and 35 non CHD were checked, laboratory test to measure the levels of Interleukin-1β was checked with enzyme-linked immunosorbent assay (ELISA). Results: There was no significant differences between interleukin-1β levels in CHD and non-CHD patients (p>0.05); there was no significant difference between the level of Interleukin-1β with periodontal status in CHD and control (non CHD) patients (p>0.05). Conclusions: levels of Interleukin-1β in CHD patients do not have a relationships with plaque index, pocket depth and clinical attachment loss, but has a relationships with bleeding on probing.

  13. Asociación entre niveles de TNF-α en fluido crevicular gingival de dientes con periodontitis apical asintomática Levels of TNF-α increase in gingival crevicular fluid of teeth with asymptomatic apical periodontitis

    Directory of Open Access Journals (Sweden)

    M Garrido Flores

    2011-12-01

    Full Text Available Introducción: Como consecuencia de necrosis pulpar séptica, el ingreso de productos bacterianos en el periápice induce la producción de citoquinas pro-inflamatorias como el Factor de Necrosis Tumoral (TNF- a, que entre otras funciones participa en la diferenciación y activación de los osteoclastos para inducir reabsorción ósea, fenómeno característico de la periodontitis apical asintomática (PAA. El fluido crevicular gingival (FCG ofrece un gran potencial como fuente de factores asociados con la actividad osteoclástica. El objetivo del presente estudio fue determinar los niveles de TNF- a en FCG de dientes con PAA y controles sanos contralaterales. Métodos: Se incluyeron 14 pacientes en la Clínica de Endodoncia de la Facultad de Odontología de la Universidad de Chile con diagnóstico clínico de PAA y se obtuvieron muestras de FCG con tiras de papel a partir de los dientes afectados y sanos contralaterales por un periodo estandarizado de 30 segundos. Se determinaron las concentraciones de proteínas totales mediante el método del ácido bisciconitico y los niveles de TNF- a, mediante ensayo ELISA. Los datos se analizaron con Test-t pareado utilizando el programa StataV11. Resultados: Se detectaron niveles de TNF- a significativamente mayores en el FCG de dientes con PAA estandarizados, tanto por 30 segundos de toma de muestra como por mg de proteínas totales. Conclusiones: Este estudio provee evidencia preliminar de que los niveles de TNF- a en el FCG reflejan la presencia de PAA y podría ser de utilidad como complemento al diagnóstico clínico y monitoreo del estado de salud o enfermedad de los tejidos perirradiculares.Introduction: As a consequence of septic pulp necrosis, the entry of bacterial products into periapical tissues induces the release of pro-inflammatory cytokines, such as the tumor necrosis factor (TNF- a. This pleiotropic cytokine is involved in the differentiation and activation of osteoclasts to induce bone

  14. Pro-oxidant status and matrix metalloproteinases in apical lesions and gingival crevicular fluid as potential biomarkers for asymptomatic apical periodontitis and endodontic treatment response

    Directory of Open Access Journals (Sweden)

    Dezerega Andrea

    2012-03-01

    Full Text Available Abstract Background Oxidative stress and matrix metalloproteinases -9 and -2 are involved in periodontal breakdown, whereas gingival crevicular fluid has been reported to reflect apical status. The aim of this study was to characterize oxidant balance and activity levels of MMP -2 and -9 in apical lesions and healthy periodontal ligament; and second, to determine whether potential changes in oxidant balance were reflected in gingival crevicular fluid from asymptomatic apical periodontitis (AAP-affected teeth at baseline and after endodontic treatment. Methods Patients with clinical diagnosis of AAP and healthy volunteers having indication of tooth extraction were recruited. Apical lesions and healthy periodontal ligaments, respectively, were homogenized or processed to obtain histological tissue sections. Matrix metalloproteinase -9 and -2 levels and/or activity were analyzed by Immunowestern blot, zymography and consecutive densitometric analysis, and their tissue localization was confirmed by immunohistochemistry. A second group of patients with AAP and indication of endodontic treatment was recruited. Gingival crevicular fluid was extracted from AAP-affected teeth at baseline, after endodontic treatment and healthy contralateral teeth. Total oxidant and antioxidant status were determined in homogenized tissue and GCF samples. Statistical analysis was performed using STATA v10 software with unpaired t test, Mann-Whitney test and Spearman's correlation. Results Activity of MMP-2 and MMP-9 along with oxidant status were higher in apical lesions (p Conclusions Apical lesions display an oxidant imbalance along with increased activity of matrix metalloproteinase-2 and -9 and might contribute to AAP progression. Oxidant imbalance can also be reflected in GCF from AAP-affected teeth and was restored to normal levels after conservative endodontic treatment. These mediators might be useful as potential biomarkers for chair-side complementary diagnostic

  15. Actividad de metaloproteinasa de matriz extracelular-9 en fluido crevicular gingival durante la respuesta reparativa en periodontitis apical asintomática

    OpenAIRE

    Obregón Miano,Fabián A; Garrido, Mauricio; Dezerega,Andrea; Hernández Ríos,Marcela

    2015-01-01

    Objetivo Los procesos destructivos del periodonto apical están en su mayoría mediados por proteasas específicas. Existe evidencia de que los niveles de metaloproteinasa de matriz extracelular-9 (MMP-9) en el fluido crevicular gingival (FCG) podrían reflejar la presencia de periodontitis apical asintomática (PAA). El objetivo de este estudio fue evaluar la actividad de MMP-9 durante la respuesta reparativa periapical en controles postendodóncicos entre una semana y 6 meses y en controles sanos...

  16. Detección de herpesvirus y bacterias periodontopatógenas en muestras de fluido crevicular gingival y saliva en periodontitis crónica

    OpenAIRE

    Herrero Sánchez, Alicia

    2015-01-01

    En los últimos 15 años, se ha sugerido que ciertos herpesvirus, en particular, el citomegalovirus (CMV) y el virus de Epstein-Barr (VEB), podrían tener cierto papel en el inicio y progresión de las enfermedades periodontales. Sin embargo, en la mayoría de los trabajos, no se han podido obtener resultados concluyentes. Por otro lado, aunque el método de referencia actual para el estudio microbiológico de los patógenos periodontales es la toma de muestras en fluido crevicular gingival (FCG), se...

  17. Efecto antiinflamatorio de apitoxina de Apis mellifera sobre prostaglandina E2 del fluido crevicular gingival de pacientes con y sin enfermedad periodontal, sometidos a apiterapia: ensayo preliminar

    OpenAIRE

    W Faúndez Poblete; Narváez Carrasco,CG; Burgos Arias,A

    2011-01-01

    Introducción: La apitoxina que es producida por la Apis mellifera posee efecto antiinflamatorio sobre una serie de marcadores biológicos. La prostaglandina E2 forma parte de ellos, estando presente en el fluido gingival crevicular (FGC). La prostaglandina E2 es evidenciada en la enfermedad periodontal. Objetivo: En este estudio se evaluó el efecto antiinflamatorio de la apitoxina sobre la concentración de prostaglandina E2 del FGC de un paciente sin enfermedad periodontal (SEP) y otro con enf...

  18. Asociación entre niveles de TNF-α en fluido crevicular gingival de dientes con periodontitis apical asintomática

    OpenAIRE

    M Garrido Flores; Ordenes Vitali,T; Segú Cabrera,C; M Baeza Paredes; J García-Sesnich; Hernández Ríos,M

    2011-01-01

    Introducción: Como consecuencia de necrosis pulpar séptica, el ingreso de productos bacterianos en el periápice induce la producción de citoquinas pro-inflamatorias como el Factor de Necrosis Tumoral (TNF)- a, que entre otras funciones participa en la diferenciación y activación de los osteoclastos para inducir reabsorción ósea, fenómeno característico de la periodontitis apical asintomática (PAA). El fluido crevicular gingival (FCG) ofrece un gran potencial como fuente de factores asociados ...

  19. The effect of subgingival antimicrobial therapy on the levels of stromelysin and tissue inhibitor of metalloproteinases in gingival crevicular fluid.

    Science.gov (United States)

    Pourtaghi, N; Radvar, M; Mooney, J; Kinane, D F

    1996-09-01

    Recent investigations imply that a key mechanism in the pathogenesis of periodontal disease may be the ability of oral microorganisms to induce production and/or activation of matrix metalloproteinases (MMPs) in the host tissues. It has been suggested that the pharmacologic inhibition of MMP activity could play an important role in achieving a desirable outcome in periodontal therapy. The efficacy of locally delivered antibiotics on the level of gingival crevicular fluid (GCF) stromelysin (SL) and tissue inhibitor of metalloproteinases (TIMP) on sites with a history of a poor response to mechanical treatment was studied. Fifty-two patients with 4 periodontal pockets > or = 5 mm and bleeding on probing were randomized into four groups of 13 patients. One group received scaling and root planing alone and the other three groups received scaling and root planing plus a locally delivered antimicrobial system. These included 25% tetracycline fiber, 2% minocycline gel, and 25% metronidazole gel. The GCF samples taken at baseline and 6 weeks after treatments were analyzed using an enzyme linked immunosorbent assay (ELISA). GCF SL levels significantly decreased after adjunctive tetracycline fiber (paired t-test, P = 0.020) and minocycline gel (paired t-test, P = 0.023) treatments whereas it remained almost unchanged in the other two groups. While the GCF TIMP level did not change significantly in the scaling and root planing alone group, it significantly increased for all three adjunctive antimicrobial treatments (for tetracycline fiber P family, may offer an advantage in changing the metalloproteinase profile of the GCF to one more compatible with periodontal health.

  20. Correlation Between Arteriosclerosis and Periodontal Condition Assessed by Lactoferrin and α1-Antitrypsin Levels in Gingival Crevicular Fluid.

    Science.gov (United States)

    Hayashi, Shuji; Yamada, Hirotsugu; Fukui, Makoto; Ito, Hiro-o; Sata, Masataka

    2015-01-01

    Patients with periodontal disease exhibit exacerbated atherosclerosis, aortic stiffness, or vascular endothelial dysfunction. However, in a recent scientific statement, the American Heart Association noted that neither has periodontal disease been proven to cause atherosclerotic vascular disease nor has the treatment of periodontal disease been proven to prevent atherosclerotic vascular disease. Therefore, the aim of the present study was to examine the correlation between periodontal condition and arteriosclerosis in patients with coronary artery disease (CAD), which is usually accompanied by systemic arteriosclerosis.We measured levels of gingival crevicular fluid lactoferrin (GCF-Lf) and α1-antitrypsin (GCF-AT) in 72 patients (67 ± 8 years, 56 men) with CAD. Furthermore, we evaluated the maximum intima-media thickness (max IMT) and plaque score of the carotid arteries as well as brachial-ankle pulse wave velocity (baPWV) and flow-mediated dilation (FMD) of the brachial artery, each of which is a parameter for determining arteriosclerosis status. The average level of GCF-Lf was 0.29 ± 0.36 µg/mL and that of GCF-AT was 0.31 ± 0.66 µg/mL, with significant correlation between the two (r = 0.701, P < 0.001). No significant difference in GCF-Lf and GCF-AT levels was observed between patients with single-, double-, and triple-vessel CAD. There were no significant correlations between the arteriosclerosis parameters (ie, max IMT, plaque score, baPWV, and FMD) and GCF-Lf or GCF-AT.No correlation between the GCF biomarkers and the severity of arteriosclerosis was detected. This result may suggest that worsening of the periodontal condition assessed by GCF biomarkers is not a major potential risk factor for arteriosclerosis.

  1. Glycated albumin and calprotectin levels in gingival crevicular fluid from patients with periodontitis and type 2 diabetes.

    Science.gov (United States)

    Kajiura, Yukari; Bando, Mika; Inagaki, Yuji; Nagata, Toshihiko; Kido, Jun-ichi

    2014-12-01

    Patients with diabetes mellitus (DM) have a high prevalence of periodontitis. Periodontitis in these patients is characterized by severe inflammation and tissue breakdown, and its diagnosis is important for cures of periodontitis and DM. The purpose of this study is to investigate the levels of glycated albumin (GA), a DM marker, and calprotectin, an inflammatory marker, in gingival crevicular fluid (GCF) from patients with periodontitis and DM (DM-P). The 78 participants in this study were patients with DM, chronic periodontitis (CP), DM-P, and healthy individuals (H). GCF and blood were collected, and GA and calprotectin in GCF were analyzed using Western blotting and enzyme-linked immunosorbent assay. Levels were compared among H, DM, CP, and DM-P groups. Blood GA and glycated hemoglobin (HbA1c) were measured, and the correlation among GCF GA and blood HbA1c or GA levels was investigated. Receiver operating characteristic (ROC) analysis for GCF GA to predict DM was performed. GA was identified in GCF, and its amount/concentration in GCF samples from DM and DM-P were significantly higher than those of non-DM groups (H and CP). Calprotectin amounts in GCF from CP and DM-P were significantly higher than in H and DM groups. GCF GA level was positively correlated with blood HbA1c and GA level. ROC analysis of GCF GA showed an optimal cutoff value to predict DM. GA showed a high level in GCF from patients with DM. Examination of GA and calprotectin in GCF may be useful for predicting DM-P.

  2. Gingival Crevicular Fluid and Salivary Periostin Levels in Non-Smoker Subjects With Chronic and Aggressive Periodontitis : Periostin Levels in Chronic and Aggressive Periodontitis.

    Science.gov (United States)

    Aral, Cüneyt A; Köseoğlu, Serhat; Sağlam, Mehmet; Pekbağrıyanık, Tuğba; Savran, Levent

    2016-06-01

    Periostin, an extracellular matrix protein functioning as an important structural mediator and adhesion molecule, has been shown to be an important regulator of connective tissue integrity. This study aimed to evaluate the levels of periostin in chronic periodontitis (CP) and aggressive periodontitis (AgP) compared to non-periodontitis (NP). Individuals were submitted to gingival crevicular fluid (GCF) and saliva sampling. Periodontal examination consisted of plaque index (PI), gingival index (GI), probing depth (PD), bleeding on probing (BOP), and clinical attachment level (CAL) measurements. Assays for periostin were performed by an enzyme-linked immunosorbent assay. Periodontitis patients presented more severe clinical indices compared to the NP group (p periodontitis. The results suggest that subjects with CP and AgP exhibit a different periostin profile. Periostin in GCF may have a protective role against periodontal disease. Furthermore, salivary periostin concentrations may have a promising diagnostic potential for the aggressive forms of periodontal disease.

  3. The detection of subgingival microflora contents of type 2 diabetes patients with periodontitis and its correlation with gingival crevicular fluid cytokine levels

    Institute of Scientific and Technical Information of China (English)

    Ai-Hua Luo; Qian Chen; Huan Zhao; Ping Xu; Yong-Xiang Fan; Ji Shi

    2015-01-01

    Objective: To study subgingival microflora contents of type 2 diabetes patients with periodontitis and its correlation with gingival crevicular fluid cytokine levels. Methods: Type 2 diabetes patients with periodontitis and simple periodontitis patients treated in our hospital from March 2012 to August 2014 were selected for study and enrolled in diabetes with periodontitis group and simple periodontitis group respectively. Then subgingival microflora contents and levels of cytokines in gingival crevicular fluid were detected. Results: (1) Subgingival microflora: compared with simple periodontitis group, contents of porphyromonas gingivalis aeromonas, actinomycetes with haemophilus, Forsyth Tanner bacteria, middle Prairie Waugh bacteria and Treponema denticola of diabetes with periodontitis group were higher;(2) Antioxidants and oxidation products: compared with simple periodontitis group, MPO, CAT, SOD, GSH and VitC contents of diabetic with periodontitis group were lower; MDA and 8-OhdG contents were higher; (3) Signaling molecules: compared with simple periodontitis group, SFRP1, Fas, FasL, Wnt5a, NF-kb and p38 contents of diabetic with periodontitis group were higher. Conclusion: Contents of pathogens in subgingival microflora of type 2 diabetes patients with periodontitis significantly increase; antioxidants are extensively consumed and oxidation products are largely generated; there is dysfunction of multiple signaling pathways.

  4. Efecto antiinflamatorio de apitoxina de Apis mellifera sobre prostaglandina E2 del fluido crevicular gingival de pacientes con y sin enfermedad periodontal, sometidos a apiterapia: ensayo preliminar Anti-inflammatory effect of apitoxin and Apis mellifera on prostaglandin E2 in gingival crevicular fluid of patients with and without periodontal disease, submitted to apitherapy: preliminary test

    OpenAIRE

    W Faúndez Poblete; CG Narváez Carrasco; A Burgos Arias

    2011-01-01

    Introducción: La apitoxina que es producida por la Apis mellifera posee efecto antiinflamatorio sobre una serie de marcadores biológicos. La prostaglandina E2 forma parte de ellos, estando presente en el fluido gingival crevicular (FGC). La prostaglandina E2 es evidenciada en la enfermedad periodontal. Objetivo: En este estudio se evaluó el efecto antiinflamatorio de la apitoxina sobre la concentración de prostaglandina E2 del FGC de un paciente sin enfermedad periodontal (SEP) y otro con enf...

  5. Evaluation and comparison of interleukin-8 (IL-8 level in gingival crevicular fluid in health and severity of periodontal disease: A clinico-biochemical study

    Directory of Open Access Journals (Sweden)

    Sushma S Lagdive

    2013-01-01

    Full Text Available Background: Cytokines play an important role in the pathology associated with chronic inflammatory diseases. Because of pro-inflammatory and neutrophil chemotactic properties, the cytokines like interleukins (IL may play a significant role in the pathogenesis of periodontitis. The biological effects of IL-8 are relevant in this regard. Aim: This study was done to compare the level of this molecule in gingival crevicular fluid (GCF from patients with adult periodontitis (experimental group and from individuals with clinically healthy gingival (control group. Materials and Methods: GCF was collected from patients with adult periodontitis and clinically healthy gingival for 30 s using a Periopaper strip and the volume of the sample determined. Following elution of the fluid, assays for IL-8 were carried out by enzyme-linked immunosorbent assay (ELISA. The concentration of IL-8 was calculated in the original volume of GCF on each strip. Results: The level of IL-8 in the experimental group was significantly higher than in the control group ( P < 0.01. The clinical parameters were positively correlated to IL-8, suggesting that the GCF IL-8 exhibited dynamic changes upon severity of periodontal disease ( P < 0.05. Conclusion: These data suggest that level of IL-8 is associated with periodontal status. The level of IL-8 in GCF is valuable in detecting the inflammation of periodontal tissue.

  6. Association of peripheral arterial disease with periodontal disease: analysis of inflammatory cytokines and an acute phase protein in gingival crevicular fluid and serum.

    Science.gov (United States)

    Çalapkorur, M Unlu; Alkan, B A; Tasdemir, Z; Akcali, Y; Saatçi, E

    2017-06-01

    Inflammation is a common feature of both peripheral arterial disease (PAD) and periodontal disease. The aim of this study was to evaluate the relationship between PAD and periodontal disease by examining the levels of inflammatory cytokines (pentraxin 3 and interleukin 1β) and high sensitive C-reactive protein from gingival crevicular fluid and serum. A total of 60 patients were included in this cross-sectional study. Patients were divided into two groups based on ankle-brachial index values: with PAD (test group) and non-PAD (control group). Demographic evaluations, clinical periodontal examinations and biochemical analysis for pentraxin 3, interleukin 1β and high sensitive C-reactive protein were performed to compare the two groups. There were no significant differences with respect to gender, age, body mass index, or smoking history (duration, amount) between the two groups (p > 0.05). There were no significant differences between the two groups in terms of clinical periodontal parameters (p > 0.05). Neither gingival crevicular fluid nor serum levels of the cytokines showed differences between the two groups. Logistic regression analysis revealed that, after adjusting for confounding factors (age, gender, diabetes, hypertension and body mass index), periodontitis raised the odds ratio for having PAD to 5.842 (95% confidence interval: 1.558-21.909). Although there were no significant differences with respect to clinical periodontal parameters and biochemical analyses between the study group and control, periodontitis did raise the odds ratio for having PAD. To clarify this possible relationship, future prospective studies are needed. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  7. Gingival crevicular fluid and serum levels of APRIL, BAFF and TNF-alpha in rheumatoid arthritis and osteoporosis patients with periodontal disease.

    Science.gov (United States)

    Gümüş, Pinar; Buduneli, Eralp; Biyikoğlu, Başak; Aksu, Kenan; Saraç, Fulden; Buduneli, Nurcan; Lappin, David F

    2013-10-01

    This study was performed to evaluate gingival crevicular fluid (GCF) and serum levels of a proliferation inducing ligand (APRIL) and B cell activating factor (BAFF) and compare this to differences between TNF-alpha levels in rheumatoid arthritis (RA), osteoporosis (OPR) and systemically healthy women with periodontal disease (SH). Gingival crevicular fluid (GCF) and serum samples were obtained before any periodontal intervention from 17 RA, 19 OPR patients and 13 SH women with periodontitis. Full-mouth clinical periodontal measurements were recorded. APRIL, BAFF and TNF-α levels were determined by ELISA. Statistical analysis was performed using multivariate analysis, ANOVA and Spearman correlation. Pocket depths differed in site-specific comparisons, but otherwise clinical measurements were similar in the three study groups. Multivariate least squares regression ANOVA adjusted for age and for plaque index indicated that total amounts of TNF-α and concentrations of TNF-α, BAFF and APRIL were significantly greater in the RA patients than in the SH group (p<0.05), and GCF concentrations of BAFF were greater in OPR patients than in SH. Serum TNF-α and BAFF were significantly higher in the RA group compared to SH (p<0.05) and serum TNF-α was greater in RA than in OPR (p<0.05). APRIL and BAFF correlated with RANKL levels in GCF and serum (p<0.05). Despite long-term usage of anti-inflammatory drugs in the RA and OPR patients, increased TNF-family cytokines, might suggest that these patients have a propensity to overproduce these inflammatory mediators but whether this results from greater disease activity or contribute to greater disease activity remains moot. Copyright © 2013 Elsevier Ltd. All rights reserved.

  8. Evaluation of the Relationship between Interleukin 17 Levels in Gingival Crevicular Fluid from Healthy Individuals and Patient with Moderate Chronic Periodontitis

    Directory of Open Access Journals (Sweden)

    Faranak Farsam

    2016-12-01

    Full Text Available Background and Aim : Chronic periodontitis is an inflammatory disease that affect the tooth-supporting tissue. It is occurs as a result of the interaction between periodontopathic bacteria and cells of host immune system. There is increasing evidence that the cytokine interleukin- 17 play a role in progression of chronic periodontitis but the exact role of this cytokine is controversial. Thus the aim of this study is evaluation of relationship between interleukin-17 level in GCF(Gingival Crevicular fluid with moderate chronic periodontitis. Materials and Methods : A total of 60 individual (n=30 per group were recruited for the study. Group 1 included 30 individual with healthy gingiva and Group 2 included 30 moderate chronic periodontitis patient.GCF samples collected in two groups.IL-17 levels were estimated in GCF using enzyme-linked immunosorbentassay(ELIZA. Results : The mean concentration of IL-17 was significantly higher in group 2 when compared with group 1.GI(Gingival index and CAL(Clinical Attachment Loss were significantly higher in group 2 when compared with group 1. Conclusion: The more level of IL- 17 in GCF in patients with chronic periodontitis suggest that IL – 17 is involved in the periodontal inflammatory process.

  9. Effect of chronic periodontitis on serum and gingival crevicular fluid oxidant and antioxidant status in patients with familial Mediterranean fever before and after periodontal treatment.

    Science.gov (United States)

    Bostanci, Vildan; Toker, Hulya; Senel, Soner; Ozdemir, Hakan; Aydin, Huseyin

    2014-05-01

    The aim of this study is to investigate the impact of periodontal status on oxidant/antioxidant status in patients with chronic periodontitis (CP) who experienced familial Mediterranean fever (FMF) and their response to non-surgical periodontal therapy. Data were obtained from 13 patients with FMF with generalized CP (FMF-CP), 15 systemically healthy patients with generalized CP, 15 systemically and periodontal healthy controls (HCs), and 14 periodontally healthy patients with FMF (FMF-HC). Each participant's total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI) in their gingival crevicular fluid (GCF) and serum were recorded. Probing depth, clinical attachment level, and gingival and plaque indices in each participant were also measured. The GCF and clinical parameters at baseline and 6 weeks after periodontal treatment were recorded. The study showed statistically significant improvement of clinical parameters in both FMF-CP and CP groups after periodontal treatment. The baseline GCF-TOS and OSI levels were significantly higher in the CP group compared with the FMF-CP group (P treatment, the GCF-TOS levels were significantly reduced in members of the FMF-CP group (P 0.05). The results of the present study show that patients with FMF-CP displayed reduced oxidative stress and increased antioxidant status compared with those in the CP and HC groups.

  10. Study of TNF-α, IL-1β and LPS levels in the gingival crevicular fluid of a rat model of diabetes mellitus and periodontitis.

    Science.gov (United States)

    Jiang, Zhu-Ling; Cui, Yu-Qiong; Gao, Rui; Li, Ying; Fu, Zhao-Chen; Zhang, Bin; Guan, Cheng-Chao

    2013-01-01

    In this study, we sought to investigate the dynamic changes in the levels of TNF-α, IL-1β and LPS in the gingival crevicular fluid (GCF) in a rat model of diabetes mellitus (DM) and periodontitis (PD). Additionally, we evaluated alveolar bone loss and the histopathological response associated with experimental diabetes mellitus and experimental periodontitis. DM and PD were induced together in 15 rats (group 1) by streptozotocin injection and ligature induction. Periodontitis alone was produced by ligature induction in 15 rats (group 2), diabetes alone was produced by streptozotocin injection in 15 rats (group 3), and fifteen systemically and periodontally healthy rats were used as controls (group 4). The gingival TNF-α, IL-1β and LPS levels were measured by using ELISA method. Periodontal destruction was assessed by measuring the alveolar bone loss. Periodontal inflammation was quantified by histopathological grading in H&E stained samples. Higher levels of TNF-α, IL1-β and LPS, increased alveolar bone loss and more serve histopathology were found in group 1 compared with group 2, group 3 and group 4 (pperiodontal tissues. The resorption values of alveolar bone and the histological inflammation were more severe in rats with periodontitis and diabetes mellitus than in those with periodontitis alone, diabetes mellitus alone and control rats. Our findings are consistent with the hypothesis that hyperglycemia contributes to the heightened inflammatory response associated with periodontitis.

  11. 糖尿病合并牙周病患者龈沟液的研究进展%Research progress on gingival crevicular fluid in patients with diabetes mellitus and periodontal diseases

    Institute of Scientific and Technical Information of China (English)

    黄婧; 梁景平

    2013-01-01

      糖尿病与牙周病两者间存在相互影响的观点已获得普遍的认可,但其具体机制还不甚明了。龈沟液由于其来源于血清和牙龈组织间质,因此能一定程度地反映组织局部的炎症情况。且由于其获取简便、无创,近年来已成为研究糖尿病与牙周病关系的热点对象。本文将就糖尿病合并牙周病患者龈沟液方面的研究展开综述。%  Although the interaction of diabetes mellitus and periodontitis was generally accepted, the mechanism is still not identified. Gingival crevicular fluid can reflect the inflammation of the periodontal tissue because it originates from the serum and interstitium. In addition, the simple and noninvasive access of gingival crevicular fluid made it become the hot spots in the researches on the relationship between diabetes mellitus and periodontal diseases in re-cent years. This article aims to review the researches of gingival crevicular fluid in patients with diabetes mellitus and periodontal diseases.

  12. Gingivitis

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/article/001056.htm Gingivitis To use the sharing features on this page, please enable JavaScript. Gingivitis is inflammation of the gums. Causes Gingivitis is ...

  13. Comparison of matrix metalloproteinase-3 and tissue inhibitor of matrix metalloproteinase-1 levels in gingival crevicular fluid in periodontal health, disease and after treatment: A clinico biochemical study

    Directory of Open Access Journals (Sweden)

    P Mohan Kumar

    2013-01-01

    Full Text Available Background: Considering the role of matrix metalloproteinase-3 (MMP-3 and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1 in the pathogenesis of periodontitis, the present study is to estimate the levels of MMP-3 and TIMP-1 in gingival crevicular fluid (GCF in periodontal health, disease and to evaluate the effect of periodontal therapy on MMP-3 and TIMP-1 concentrations in GCF. Materials and Methods: A periodontal examination and collection of GCF by extra-crevicular method was performed in 30 subjects selected randomly and categorized into 3 groups. Group I consists of 10 subjects Group II consists of 20 patients and Group III consists of 20 patients of Group II. Non surgical periodontal therapy was performed, and GCF was collected after 8 weeks from the same site of 20 chronic periodontitis patients who are considered as Group III. MMP- 3 and TIMP-1 levels were estimated in GCF-samples by using enzyme-linked immunosorbent assay. The findings were analyzed using the software and descriptive statistical methods such as Mann- Whitney U-test and Kruskal-Wallis test. P value < 0.001 was considered significant. Results: MMP-3 and TIMP-1 was detected in all samples. Highest mean MMP-3 concentrations in GCF were obtained for Group II (7.490 ng/ml while the lowest concentrations were seen in Group I (0.344 ng/ml and Group III (2.129 ng/ml. This suggests that MMP-3 levels in GCF increases proportionally with the progression of periodontal disease and decreases after treatment. Lowest mean TIMP-1 concentrations in GCF were obtained for Group-II (1.592 ng/ml, while the highest concentrations were seen in Group-I (8.78 ng/ml and Group-III (6.40 ng/ml. This suggests that TIMP-1 levels in GCF decreases proportionally with progression of periodontal disease and increases after treatment. Conclusion: There is a substantial increase in the concentrations of MMP-3 and decrease in TIMP-1 as periodontal disease progress. Since MMP-3 and TIMP-1 levels

  14. The Effect of Photodynamic Therapy and Diode Laser as Adjunctive Periodontal Therapy on the Inflammatory Mediators Levels in Gingival Crevicular Fluid and Clinical Periodontal Status

    Science.gov (United States)

    Teymouri, Faraz; Farhad, Shirin Zahra; Golestaneh, Hedayatollah

    2016-01-01

    Statement of the Problem The presence of bacterial biofilms is the major cause of gingivitis and periodontitis, their mechanical removal is not often enough. Therefore, laser therapy and photodynamic therapy can be effective as adjunctive treatment. Purpose This study aimed to evaluate the impact of these treatments on the level of gingival crevicular fluid (GCF), inflammatory mediators, and periodontal clinical status. Materials and Method In this clinical trial, three quadrants were studied in 12 patients with chronic periodontitis aged 30-60 years. The clinical parameters were recorded and GCF samples were taken. After the first phase of periodontal treatment, one of the three quadrants was determined as the control group, one was treated by diode laser, and one underwent photodynamic therapy. The clinical parameters were recorded 2 and 6 weeks later. The data were statistically analyzed by using Friedman, ANOVA, and LSD post-test. Results Significant reduction was observed over time in the level of Interleukin-1β (IL-1β), Interleukin-17 (IL-17), clinical attachment loss, and pocket depth in the three treatment groups (p< 0.000). The three treatment methods significantly reduced the IL-1β and IL-17 at the baseline, up to 2 weeks, and 2-6 weeks (p< 0.05). Diode laser and photodynamic therapy significantly decreased the average bleeding on probing over time (p< 0.000 and p< 0.002, respectively). Conclusion Laser and photodynamic therapy reduced the inflammatory mediators (IL-1β and IL-17) and improved the clinical symptoms. PMID:27602399

  15. The Effect of Photodynamic Therapy and Diode Laser as Adjunctive Periodontal Therapy on the Inflammatory Mediators Levels in Gingival Crevicular Fluid and Clinical Periodontal Status

    Directory of Open Access Journals (Sweden)

    Faraz Teymouri

    2016-09-01

    Full Text Available Statement of the Problem: The presence of bacterial biofilms is the major cause of gingivitis and periodontitis, their mechanical removal is not often enough. Therefore, laser therapy and photodynamic therapy can be effective as adjunctive treatment. Purpose: This study aimed to evaluate the impact of these treatments on the level of gingival crevicular fluid (GCF, inflammatory mediators, and periodontal clinical status. Materials and Method: In this clinical trial, three quadrants were studied in 12 patients with chronic periodontitis aged 30-60 years. The clinical parameters were recorded and GCF samples were taken. After the first phase of periodontal treatment, one of the three quadrants was determined as the control group, one was treated by diode laser, and one underwent photodynamic therapy. The clinical parameters were recorded 2 and 6 weeks later. The data were statistically analyzed by using Friedman, ANOVA, and LSD post-test. Results: Significant reduction was observed over time in the level of Interleukin-1β (IL-1β, Interleukin-17 (IL-17, clinical attachment loss, and pocket depth in the three treatment groups (p< 0.000. The three treatment methods significantly reduced the IL-1β and IL-17 at the baseline, up to 2 weeks, and 2-6 weeks (p< 0.05. Diode laser and photodynamic therapy significantly decreased the average bleeding on probing over time (p< 0.000 and p< 0.002, respectively. Conclusion: Laser and photodynamic therapy reduced the inflammatory mediators (IL-1β and IL-17 and improved the clinical symptoms.

  16. Proinflammatory and anti-inflammatory cytokines in gingival crevicular fluid and serum of patients with rheumatoid arthritis and patients with chronic periodontitis.

    Science.gov (United States)

    Cetinkaya, Burcu; Guzeldemir, Esra; Ogus, Ersin; Bulut, Sule

    2013-01-01

    The aim of this study is to evaluate proinflammatory and anti-inflammatory cytokine levels in gingival crevicular fluid (GCF) and serum of rheumatoid arthritis (RA) and chronic periodontitis (CP) patients to assess whether cytokine profiles distinguish patients with RA and patients with CP while using healthy patients as background controls. A total of 49 patients, 17 patients with RA (three males and 14 females; mean age: 47.82 ± 10.74 years), 16 patients with CP (10 males and six females; mean age: 44.00 ± 7.00 years), and 16 controls (eight males and eight females; mean age: 28.06 ± 6.18 years) were enrolled. Patients with RA were under the supervision of rheumatologists; 15 of the patients with RA were being treated with methotrexate-sulfasalazine combined therapy, and two of the patients were being treated with leflunomid therapy. Periodontal parameters (plaque index, gingival index, probing depth, and clinical attachment level) were recorded. Interleukin (IL)-1β, IL-4, IL-10, and tumor necrosis factor-α (TNF-α) were determined in GCF and IL-1β and IL-10 in serum by enzyme-linked immunosorbent assay. There were significant differences found among RA, CP, and control groups for all periodontal parameters (P 0.05). Although the total amount and concentration of serum IL-10 was not significantly different among the groups (P >0.05), serum IL-1β was significantly lower in the RA group compared to CP patients and controls and was higher in GCF of the RA group compared to the CP group. Although clinical periodontal disease parameters indicated more severe periodontal disease in CP compared to RA patients, immunologic evaluation did not reveal consistent results regarding proinflammatory and anti-inflammatory cytokine levels. This might be a result of the use of non-steroidal anti-inflammatory drugs and rheumatoid agents by patients with RA.

  17. Effect of the use of snuff on the levels of interleukin-1 β and interleukin-8 in the gingival crevicular fluid of periodontitis patients

    Directory of Open Access Journals (Sweden)

    Vijayendra Pandey

    2015-01-01

    Full Text Available Background: Use of smokeless tobacco in the form of moist snuff placed in the oral cavity is popular in rural India. The aim of the present cross-sectional study was to determine the effect of snuff on periodontitis by assessing interleukin (IL-1 β and IL-8 levels in gingival crevicular fluid. Materials and Methods: A total of 60 subjects were selected for this study. 40 subjects presented with periodontitis, which included 20 snuff users (SP and 20 nonsnuff users (NS. 20 periodontally healthy patients formed the controls (healthy control: HC. The clinical parameters recorded were gingival index (GI, plaque index, calculus index, bleeding on probing (BOP, probing depth (PD, recession (RC, and clinical attachment level (CAL. The IL-1 β and IL-8 levels were assessed through enzyme-linked immunosorbent assay (Quantikine ®. Analysis of variance (ANOVA, post-hoc Tukey′s, Kruskal-Walli′s ANOVA and Mann-Whitney test was used for comparison among groups and P > 0.05 was considered statistically significant. Results: No significant difference was seen in levels of IL-1 β and IL-8 between SP and NS groups (P = 0.16, 0.97. However, both the periodontitis groups (SP and NS had increased IL-β levels when compared to HC group (P = 0.01, 0.001. The snuff users showed significant increase in GI, BOP, RC, and CAL when compared with NS (P = 0.002, 0.001, 0.012, 0.002 whereas NS group had significant increase in PD (P = 0.003. Conclusion: Within the limitations of this study, use of snuff does not affect the host inflammatory response associated with periodontitis and leads to RC and increased CAL due to local irritant effect.

  18. Effect of the use of snuff on the levels of interleukin-1 β and interleukin-8 in the gingival crevicular fluid of periodontitis patients.

    Science.gov (United States)

    Pandey, Vijayendra; Salam, Sharib Abdus; Moda, Aman; Agarwal, Preeti; Nath, Sonia; Pulikkotil, Shaju Jacob

    2015-01-01

    Use of smokeless tobacco in the form of moist snuff placed in the oral cavity is popular in rural India. The aim of the present cross-sectional study was to determine the effect of snuff on periodontitis by assessing interleukin (IL)-1 β and IL-8 levels in gingival crevicular fluid. A total of 60 subjects were selected for this study. 40 subjects presented with periodontitis, which included 20 snuff users (SP) and 20 nonsnuff users (NS). 20 periodontally healthy patients formed the controls (healthy control: HC). The clinical parameters recorded were gingival index (GI), plaque index, calculus index, bleeding on probing (BOP), probing depth (PD), recession (RC), and clinical attachment level (CAL). The IL-1 β and IL-8 levels were assessed through enzyme-linked immunosorbent assay (Quantikine(®)). Analysis of variance (ANOVA), post-hoc Tukey's, Kruskal-Walli's ANOVA and Mann-Whitney test was used for comparison among groups and P > 0.05 was considered statistically significant. No significant difference was seen in levels of IL-1 β and IL-8 between SP and NS groups (P = 0.16, 0.97). However, both the periodontitis groups (SP and NS) had increased IL-β levels when compared to HC group (P = 0.01, 0.001). The snuff users showed significant increase in GI, BOP, RC, and CAL when compared with NS (P = 0.002, 0.001, 0.012, 0.002) whereas NS group had significant increase in PD (P = 0.003). Within the limitations of this study, use of snuff does not affect the host inflammatory response associated with periodontitis and leads to RC and increased CAL due to local irritant effect.

  19. Patients with dental calculus have increased saliva and gingival crevicular fluid fetuin-A levels but no association with fetuin-A polymorphisms.

    Science.gov (United States)

    Doğan, Gülnihal Emrem; Demir, Turgut; Laloğlu, Esra; Sağlam, Ebru; Aksoy, Hülya; Yildirim, Abdulkadir; Akçay, Fatih

    2016-12-22

    Fetuin-A is a potent inhibitor of calcium-phosphate precipitation and of the calcification process, therefore it can also be related with dental calculus. Thus, we aimed to investigate a possible relationship between fetuin-A gene polymorphism and the presence of dental calculus. A possible relationship between serum, saliva and gingival crevicular fluid (GCF) levels of fetuin-A was also investigated. Fetuin-A c.742C > T and c.766C > G polymorphisms were investigated in 103 patients with or without dental calculus. Additionally, serum, saliva and GCF fetuin-A levels of patients were compared according to dental calculus presence. A significant difference was not observed in the distribution of the fetuin-A c.742C > T and c.766C > G polymorphisms between patients with or without dental calculus. Saliva and GCF fetuin-A concentrations of patients with dental calculus were statistically higher than those without dental calculus (P=0.001, P=0.036 respectively). According to our results, fetuin-A c.742C > T and c.766C > G polymorphisms were not associated with presence of dental calculus. However, higher GCF and saliva fetuin-A levels were detected in patients with dental calculus than in patients without dental calculus, which may result from an adaptive mechanism to inhibit mineral precipitation and eventually calculus formation.

  20. The effect of adjunctive low-dose doxycycline and licorice therapy on gingival crevicular fluid matrix metalloproteinase-8 levels in chronic periodontitis

    Directory of Open Access Journals (Sweden)

    Shirin Zahra Farhad

    2013-01-01

    Full Text Available Background: This study compared the effect of adjunctive low dose doxycycline and licorice on gingival crevicular fluid (GCF matrix metalloproteinase-8 (MMP-8 levels in patients with chronic periodontitis. Materials and Methods: In this in vivo, experimental study 39 patients with mild to moderate chronic periodontitis were selected. Samples of GCF were collected from three deepest pockets and MMP-8 concentration was measured. Patients were divided into three groups (n = 13. Groups were treated with doxycycline, licorice and placebo. Sampling and measurement of MMP-8 was repeated after 6 weeks. Data was analyzed by t-paired and ANOVA test. P < 0.001 was considered significant. Results: The decrease in mean of MMP-8 concentration was higher in doxycycline and licorice group in comparison with the placebo group and the difference was statistically significant (P value < 0.001. The decrease in mean of MMP-8 concentration was higher in licorice group than doxycycline group, but the difference was not statistically significant. Conclusion: The present study showed that licorice extract can prevent the production of MMPs by host cells and can be as useful as antibiotics like doxycycline to cure periodontal and other inflammatory diseases. It must be added that no side-effects were observed in usage of licorice extract.

  1. The effects of chronic periodontitis and rheumatoid arthritis on serum and gingival crevicular fluid total antioxidant/oxidant status and oxidative stress index.

    Science.gov (United States)

    Esen, Cağrı; Alkan, Banu Arzu; Kırnap, Mehmet; Akgül, Ozgür; Işıkoğlu, Semra; Erel, Ozcan

    2012-06-01

    Chronic periodontitis (CP) and rheumatoid arthritis (RA) appear to share many pathologic features. Oxygen metabolism has an important role in the pathogenesis of both CP and RA. The aim of this study is to evaluate the relationship between these two chronic inflammatory diseases with regard to antioxidant and oxidant status. A total of 80 participants were divided into four groups of 20 each: group RA-CP (patients with RA and CP), group RA (periodontally healthy patients with RA), group CP (systemically healthy patients with CP), and group C (periodontally and systemically healthy volunteers) were included in the study. After assessment of periodontal measurements, gingival crevicular fluid (GCF) samples were taken at one incisor, premolar, and molar tooth and stored with serum samples at -80°C for the antioxidant/oxidant assay. Although all clinical measurements in groups RA-CP and CP were statistically higher compared to those of C and RA groups (P 0.05). GCF total oxidant status (TOS) values of CP and RA-CP groups were higher than those of the RA group (P oxidative stress index (OSI) values of the RA-CP group were higher than those of the RA group (P 0.05). Local OSI values in groups with patients with CP were higher, whereas systemic OSI values showed no difference among the groups. The presence of RA seems not to affect local and systemic OSI values in patients with CP.

  2. Inflammatory markers in gingival crevicular fluid of periodontitis patients with type 2 diabetes mellitus according to glycemic control: A pilot study.

    Science.gov (United States)

    Longo, Priscila Larcher; Artese, Hilana Paula Carilo; Horliana, Anna Carolina Ratto Tempestini; Gomes, Giovane Hisse; Romito, Giuseppe Alexandre; Dib, Sergio Atala; Mayer, Marcia Pinto Alves

    2015-01-01

    Type 2 diabetes mellitus (T2DM) and periodontitis are inflammatory conditions with a bidirectional association. This pilot study aimed to evaluate whether T2DM and glycemic control interfere in inflammatory markers profiles in gingival crevicular fluid (GCF) in periodontitis patients. Fourteen diabetic periodontitis patients were enrolled in this study, seven with adequate glycemic control (glycated hemoglobin [HbA1c] periodontitis patients without diabetes formed the control group (P). GCF was obtained from diseased sites (probing depth >6 mm) of an entirely hemiarch, pooled and cytokines levels determined using multiplex beads immunoassay. Clinical periodontal parameters were analyzed by Mann-Whitney test and levels of cytokines by Kruskal-Wallis and Dunn's multiple comparison tests with confidence level of 95% (P periodontal pockets presented high levels of inflammatory cytokines, and there were no statistical differences between levels of interleukin-6 (IL-6), IL-8 and tumor necrosis factor-α according to presence of diabetes or percentage of HbA1c among the groups, despite groups with T2DM and periodontitis exhibit higher levels of PD. Within the limitations of this study, inflammatory mediators in GCF are dependent to the local response and do not correlate with the diabetic status.

  3. Levels of vaspin and omentin-1 in gingival crevicular fluid as potential markers of inflammation in patients with chronic periodontitis and type 2 diabetes mellitus.

    Science.gov (United States)

    Bozkurt Doğan, Şeyma; Öngöz Dede, Figen; Ballı, Umut; Sertoğlu, Erdim

    2016-01-01

    The aims of the present study were to determine the levels of vaspin and omentin-1 in gingival crevicular fluid (GCF) in patients with chronic periodontitis (CP) with and without type 2 diabetes mellitus (T2DM), and to evaluate GCF vaspin and omentin-1 levels after non-surgical periodontal therapy. The study included 60 subjects: 15 systemically and periodontally healthy individuals, 15 periodontally healthy patients with T2DM, 15 systemically healthy patients with CP, and 15 patients with both CP and T2DM. GCF and clinical periodontal parameters were examined at the baseline and 6 weeks after periodontal therapy. Levels of vaspin, omentin-1 and tumor necrosis factor-alpha (TNF-α) were measured by ELISA, and their relative ratios were calculated. GCF vaspin and TNF-α levels were significantly higher in the CP groups than in the periodontally healthy groups (P periodontally healthy groups (P periodontal therapy influenced the GCF levels of both vaspin and omentin-1 in the CP groups. Our results suggest that the levels of vaspin and omentin-1 in GCF could have potential application as inflammatory markers of diabetes, periodontal disease and treatment outcome. (J Oral Sci 58, 379-389, 2016).

  4. Inflammatory markers in gingival crevicular fluid of periodontitis patients with type 2 diabetes mellitus according to glycemic control: A pilot study

    Directory of Open Access Journals (Sweden)

    Priscila Larcher Longo

    2015-01-01

    Full Text Available Background: Type 2 diabetes mellitus (T2DM and periodontitis are inflammatory conditions with a bidirectional association. This pilot study aimed to evaluate whether T2DM and glycemic control interfere in inflammatory markers profiles in gingival crevicular fluid (GCF in periodontitis patients. Materials and Methods: Fourteen diabetic periodontitis patients were enrolled in this study, seven with adequate glycemic control (glycated hemoglobin [HbA1c] 6 mm of an entirely hemiarch, pooled and cytokines levels determined using multiplex beads immunoassay. Clinical periodontal parameters were analyzed by Mann-Whitney test and levels of cytokines by Kruskal-Wallis and Dunn′s multiple comparison tests with confidence level of 95% (P < 0.05. Results: Cytokines profile of GCF obtained from deep periodontal pockets presented high levels of inflammatory cytokines, and there were no statistical differences between levels of interleukin-6 (IL-6, IL-8 and tumor necrosis factor-α according to presence of diabetes or percentage of HbA1c among the groups, despite groups with T2DM and periodontitis exhibit higher levels of PD. Conclusion: Within the limitations of this study, inflammatory mediators in GCF are dependent to the local response and do not correlate with the diabetic status.

  5. Effect of non-surgical periodontal treatment on visfatin concentrations in serum and gingival crevicular fluid of patients with chronic periodontitis and type 2 diabetes mellitus.

    Science.gov (United States)

    Wu, Yun; Chen, Ling; Wei, Bin; Luo, Kai; Yan, Fuhua

    2015-06-01

    This study aims to assess visfatin concentrations in serum and gingival crevicular fluid (GCF) and investigate this relationship in patients with type 2 diabetes mellitus (T2DM) and chronic periodontitis (CP) before and after non-surgical periodontal treatment. Fifty-four patients with T2DM and CP were recruited. The patients were randomly divided into two groups: treatment and control. Serum and GCF visfatin concentrations and glycated hemoglobin (HbA1c) levels were measured by enzyme-linked immunosorbent assay at different time points (at baseline and 3 and 6 months after non-surgical periodontal treatment). Serum and GCF visfatin concentrations showed no significant differences between the groups at baseline (t test, P >0.05). A significant decline of visfatin in the treatment group was found in serum and GCF 3 months after non-surgical periodontal treatment (t test, P 0.05). The data suggest that non-surgical periodontal treatment is helpful for glucose control, an effect that may be associated with reduced visfatin in patients with T2DM and periodontitis. Furthermore, the data suggest that visfatin may be considered an inflammatory marker for periodontal diseases.

  6. Matrix metalloproteinases -1, -2, -3, -7, -8, -12, and -13 in gingival crevicular fluid during orthodontic tooth movement: a longitudinal randomized split-mouth study.

    Science.gov (United States)

    Canavarro, Cristiane; Teles, Ricardo Palmier; Capelli Júnior, Jonas

    2013-10-01

    This randomized split-mouth study aimed to examine the levels of matrix metalloproteinases (MMPs) -1, -2, -3, -7, -8, -12, and -13 in the gingival crevicular fluid (GCF) at different time points during orthodontic tooth movement. A total of 16 healthy orthodontic subjects (7 females, 9 males; mean age, 17.7 years) who needed their first upper premolars extracted were enrolled. One randomly chosen maxillary canine was subjected to a distalizing force and was considered to be the test side. The contralateral canine, which was not subjected to any force but was included in the orthodontic appliance, was used as a control side. GCF sampling was performed at both the mesial (tension) and distal (pressure) test and control sites at baseline, immediately before applying the orthodontic appliance, and after 1 and 24 hours and 7, 14, and 21 days. A multiplexed bead immunoassay was used to analyse the GCF samples. The mean levels of the MMP-1, -2, -3, -7, -8, -12, and -13 were not significantly different between the test and control groups in each time showed. The comparisons between the tension and pressure sites were also not significantly different at each individual time. A few variations focused on MMP-1 and -3, but the expression of MMP-8 was higher than that of the other MMPs. MMPs are released in sufficient quantities such that tooth movement occurs but with no significant increase in GCF levels.

  7. THE ASSOCIATION OF FIXED ORTHODONTIC TREATMENT OF GINGIVAL CREVICULAR FLUID WITH PERIODONTAL HEALTH: A PRELIMINARY STUDY REPORT%固定矫治病人龈沟液量与牙周健康状况的初步研究

    Institute of Scientific and Technical Information of China (English)

    杨丽; 范存晖; 于新波; 陈秀娟

    2012-01-01

    目的 探讨固定矫治病人矫治前后龈沟液量与牙周健康状况的关系.方法 选取接受直丝弓矫治技术治疗的病人24例,以右上颌第一磨牙(16)、左上颌中切牙(21)、左下颌第一前磨牙或第二前磨牙(34或35)、右下颌中切牙(41)4个牙位为检测牙位,分别于矫治前和矫治1、3个月时,检测其龈沟液量、菌斑指数、牙龈指数和探诊深度.结果 固定矫治1个月和3个月时,病人的菌斑指数、牙龈指数和龈沟液量均较矫治前显著升高,差异有显著性(F=4.729~37.058,P<0.05);探诊深度矫治3个月时较矫治前和矫治1个月升高,差异有显著性(F=12.974,P<0.05).龈沟液量与菌斑指数、牙龈指数、探诊深度之间存在明显的正相关关系(r=0.288~0.503,P<0.05),结论 龈沟液量检测是一种简单有效的评价固定矫治病人牙周健康状况的指标.%Objective To observe the relationship between gingival crevicular fluid volume and periodontal health condition, before and after fixed orthodontic treatment. Methods Twenty-four patients wearing a straight wire appliance were included in this study. The right maxillary first molar (16), left maxillary central incisor (21), left mandibular first molar and right mandibular central incisor (41) were selected to be checked. The gingival crevicular fluid, plaque indext gingival index, and depth of probing were measured before and at one and three months after the treatment. Results At one and three months of the treatment, the plaque index, gingival index and the volume of gingival crevicular fluid in the patients increased significantly as compared with before treatment (F=4. 729 - 37.058,P<0.05) ; while probing depth significantly increased at three months of treatment (F=12. 974,P<0.05). The amount of gingival crevicular fluid was positively correlated with plaque index or gingival index or probing depth (r-0. 288 - 0. 503 ,P<0. 05). Conclusion The amount of gingival crevicular

  8. Expression of Pentraxin 3 and Thrombospondin 1 in Gingival Crevicular Fluid during Wound Healing after Gingivectomy in Postorthodontic Patients

    OpenAIRE

    Anne Marie Rauten; Isabela Silosi; Stefan Ioan Stratul; Liliana Foia; Adrian Camen; Vasilica Toma; Daniel Cioloca; Valeriu Surlin; Petra Surlin; Maria Bogdan

    2016-01-01

    Background. Wound healing is a tissue repair process after an injury, and two of its main components are inflammation and angiogenesis, in which course a cascade of mediators is involved. The aim of this research was to evaluate the involvement of Pentraxin 3 and Thrombospondin 1 in wound healing after periodontal surgery (gingivectomy) for gingival overgrowth during orthodontic treatment with or without magnification devices, by assessing their levels in GCF. Methods. From 19 patients with g...

  9. 全瓷冠修复对高血糖患者龈沟液成分影响的分析%Effect of hyperglycemia on gingival crevicular fluid after restoration of ceramic crown

    Institute of Scientific and Technical Information of China (English)

    隋晶; 李斌; 黄毓秋; 张晓燕

    2014-01-01

    Objective:To investigate the effect of hyperglycemia on gingival crevicular fluid after restoration of ceramic crown.Method:80 patients (40 male and 40 female) were randomly selected from the 40 to 50-year-old patients treated in our department in the past two years who were indicated for single ceramic crown. All the patients were treated with routine periodontal prophylaxis and restoration of all-ceramic crowns. The gross secretory volume of gingival crevicular fluid, ALP, IL-1 and TNF-αwere measured before and 2 months after restoration.Results:Among patients with normal blood glucose, there were no significant changes on gross secretory volume of gingival crevicular fluid, ALP, IL-1 and TNF-αlevels 2 months after restoration of all-ceramic crowns. Among patients with hyperglycemia, gross secretory volume of gingival crevicular fluid, IL-1 and TNF-αlevels significantly increased 2 months after restoration of all-ceramic crowns, but level of ALP did not change significantly.Conclusion:Hyperglycemia could promote gingival crevicular fluid secretion, and IL-1, TNF-αlevels in gingival crevicular fluid after restoration of ceramic crown.%目的:探讨高血糖对全瓷冠固定修复后龈沟液成分的影响。方法:选取近两年在我院口腔修复科就诊的40-50岁,需进行全瓷单冠修复的患者80例为受试对象,其中男性40例,女性40例,常规牙周洁治后采用全瓷单冠修复,分别在修复前以及修复后2个月检测基牙龈沟液的分泌量以及碱性磷酸酶,白细胞介素1β和肿瘤坏死因子α水平。结果:正常血糖患者全瓷修复后2个月基牙龈沟液分泌量,碱性磷酸酶,白细胞介素1和肿瘤坏死因子α水平均无明显升高。高糖组修复后2个月基牙龈沟液分泌量,白细胞介素1和肿瘤坏死因子α显著高于修复前,但碱性磷酸酶水平与修复前比较差异无统计学意义。结论:高糖环境可使全瓷冠修复后患者龈沟液

  10. Effects of double gingival retraction and gingival retraction on the quantity and components of gingival crevicular fluid%双线排龈法和单线排龈法对基牙龈沟液含量和成分的影响

    Institute of Scientific and Technical Information of China (English)

    孙艳燕; 匡斌

    2013-01-01

    目的:比较双线排龈和单线排龈对基牙龈沟液含量和成分的影响.方法:对47位患者的94个牙行冠桥修复,采用同一患者的不同牙位对照,分别采取双线排龈法和单线排龈法,取印模,完成修复体.于修复后7、21d和3、6个月提取受试牙位龈沟液,称重并检测其中天冬氨酸转氨酶(AST)和碱性磷酸酶(ALP)含量.结果:修复后7d,单线排龈组龈沟液量、GCF-AST和GCF-ALP含量高于双线排龈组(P<0.05);修复后21 d、3个月,两组各项牙周指标之间无显著性差异(P>0.05);修复后6个月,单线排龈组龈沟液含量高于双线排龈组(P<0.05),GCF-AST和GCF-ALP含量有所升高但差异无统计学意义(P>0.05).结论:双线排龈法对牙周组织的影响较单线排龈法小,更有利于牙周组织的健康.%AIM: To study the effects of double gingival retraction and gingival retraction before impression on the quantity and components of gingival crevicular fluid {GCF). METHODS: 94 teeth in 47 patients were treated by fixed prosthetics. Double gingival retraction was applied on one side and gingival retraction was applied on the other side in each patient. The quantity of GCF and the content of aspartate aminotransferase( AST) and alkaline phosphatase ( ALP) in GCF were assessed at 7 and 21 d, 3 and 6 months after application of the prosthetics. RESULTS: 7 d after treatment the quantity of GCE and the content of AST and ALT in the gingival retraction group were higher than those in double gingival retraction groupe ( P 0.05). 6 months after treatment the GCF in gingival retraction groupe was more than that in double gingival retraction groupe, while AST and ALT content was not statistically different between the two groups (P>0. 05). CONCLUSION; Double gingival retraction has less effect on periodontal tissues than gingival retraction.

  11. Contents comparison of AST and ALP in gingival crevicular fluid from pregnant women with or without pregnancy gingivitis%妊娠期牙龈炎患者龈沟液中天门冬氨酸转氨酶和碱性磷酸酶的含量变化

    Institute of Scientific and Technical Information of China (English)

    毛敬

    2012-01-01

    目的:比较妊娠期牙龈炎患者和正常妊娠妇女龈沟液中天门冬氨酸转氨酶(AST)和碱性磷酸酶(ALP)水平的变化,探讨AST和ALP在妊娠期牙龈炎中的作用.方法:采集30例妊娠期牙龈炎患者和30例正常妊娠妇女龈沟液,处理后采用全自动生化分析仪分析AST和ALP含量,并做统计学分析.结果:妊娠期牙龈炎患者龈沟液中AST和ALP含量均显著高于正常妊娠妇女龈沟液中的含量,有统计学差异(P<0.01).结论:在妊娠期牙龈炎的发生发展过程中,AST和ALP发挥了重要的致炎作用,AST、ALP可以作为提示炎症活跃的信号分子.%Objectine: To investigate the effect of aspartate transaminase (AST) and alkaline phosphatase (ALP) in pregnancy gingivitis by comparing the levels of AST and ALP in gingival crevicular fluid from pregnant women with or without pregnancy gingivitis. Method: Gingival crevicular fluid from 30 pregnant women with pregnancy gingivitis and 30 pregnant women without pregnancy gingivitis were harvested respectively, then analyzed the levels of AST and ALP by automatic biochemistry analyzer after special treatment and performed statistical analysis. Result: Levels of AST and ALP in gingival crevicular fluid from pregnant women with pregnancy gingivitis were significantly higher than those from pregnant women without pregnancy gingivitis and statistical difference could be detected, P <0.01. Conclusion: AST and ALP play important roles in pregnancy gingivitis and could be regard as signaling molecules of active inflammation

  12. Effect of Bisphosphonates on the Levels of Rankl and Opg in Gingival Crevicular Fluid of Patients With Periodontal Disease and Post-menopausal Osteoporosis.

    Science.gov (United States)

    Verde, María E; Bermejo, Daniela; Gruppi, Adriana; Grenón, Miriam

    2015-12-01

    The Receptor activator of nuclear factor-kappa B ligand (RANKL)/RANK/Osteoprotegerine (OPG) system has been proposed as essential for osteoclast biology and identified as key part in regulating the physiology and pathology of the skeletal system. The study of the RANKL/RANK/OPG system has increased the understanding of the mechanisms involved in the bone remodeling process, especially in postmenopausal osteoporosis and periodontal disease. Bisphosphonates have become the mainstay of the treatment and prevention of post-menopausal osteoporosis. They inhibit the formation and dissolution of calcium phosphate crystals in bone and also osteoclasts, thus reducing bone turnover.Current investigations relate osteoporosis with the appearance and progression of periodontal disease. Although the etiology of both is different, the bone loss present in both shares several characteristics. Thus, therapy used for osteoporosis can be considered of value in the treatment of periodontal disease. The aim of this study was to evaluate the levels of RANKL, OPG and their relationship in gingival crevicular fluid (GCF) in patients with periodontal disease and postmenopausal osteoporosis/ osteopenia in relation to consumption of bisphosphonates. We studied 66 periodontal active sites obtained from 17 post- menopausal women patients aged between 45-70 years old with osteoporosis/osteopenia and periodontal disease. GCF samples were collected using sterile filter paper strips. To determine the concentration of RANKL and OPG, a commercial ELISA assay was used. The values of RANKL, OPG and their ratio (RANKL/ OPG) were compared with Mann-Whitney U Test. The values of RANKL, OPG and their ratio obtained in patients with osteoporosis/osteopenia and periodontal disease with or without bisphosphonates treatment showed no differences. Bisphosphonates do not alter the concentration of RANKL and OPG and their ratio in the GCF of patients with osteoporosis/ osteopenia and periodontal disease

  13. Effect of Non-Surgical Periodontal Therapy on Interleukin-29 Levels in Gingival Crevicular Fluid of Chronic Periodontitis and Aggressive Periodontitis Patients

    Directory of Open Access Journals (Sweden)

    B. M. Shivaprasad

    2013-01-01

    Full Text Available Recently discovered interleukin 29 (IL-29 has antiviral properties and its production is induced by herpes viruses. This study was aimed at analyzing the effect of non-surgical periodontal treatment on IL-29 levels in gingival crevicular fluid (GCF of chronic and aggressive periodontitis patients. A total of 60 participants were divided into healthy group (group 1; n = 20, chronic periodontitis group (group 2; n = 20, and aggressive periodontitis group (group 3; n = 20. GCF samples collected from each subject at baseline and 6–8 weeks after scaling and root planing were quantified for IL-29 levels using ELISA. The mean IL-29 concentration in GCF was found to be highest in group 3 (92.37 pg/μl. The mean IL-29 level in group 1 and group 2 was 36.88 pg/μl and 69.35 pg/μl respectively. After scaling and root planing, the mean concentration of IL-29 in GCF was increased to 85.99 pg/μl in group 2 and to 114.64 pg/μl in group 3. Results of the present study indicate that antiviral IL-29 level was highest in GCF of aggressive periodontitis patients and least in subjects with healthy periodontium, while that of chronic periodontitis lying in between. After non-surgical periodontal therapy, IL-29 levels increased both in chronic and aggressive periodontitis patients and deserve further investigation as a potential therapeutic agent in treating periodontitis.

  14. Impact of Chronic Periodontitis on Levels of Glucoregulatory Biomarkers in Gingival Crevicular Fluid of Adults with and without Type 2 Diabetes.

    Directory of Open Access Journals (Sweden)

    Hasaan G Mohamed

    Full Text Available The relationship between diabetes and periodontal disease is bidirectional, but information about the effect of chronic periodontitis on the levels of the glucoregulatory biomarkers locally in gingival crevicular fluid (GCF is limited. The aim of this study was to compare the levels of 10 glucoregulatory biomarkers in GCF, firstly in subjects with type 2 diabetes (T2DM presenting with and without chronic periodontitis and secondly, in subjects without diabetes, with and without chronic periodontitis. The material comprised a total of 152 subjects, stratified as: 54 with T2DM and chronic periodontitis (G1, 24 with T2DM (G2, 30 with chronic periodontitis (G3 and 44 without T2DM or periodontitis (G4. The levels of the biomarkers were measured using multiplex biometric immunoassays. Periodontal pocket depths were recorded in mm. Subsets G1 and G2 and subsets G3 and G4 were compared independently. Among T2DM subjects, GIP, GLP-1 and glucagon were significantly up-regulated in G1 compared to G2. Moreover, there were no statistical differences between the two groups regarding C-peptide, insulin, ghrelin, leptin and PAI-1. Comparisons among individuals without T2DM revealed significantly lower amounts of C-peptide and ghrelin in G3 than in G4. The number of sites with pocket depth ≥ 4mm correlated negatively with C-peptide (Spearman's correlation co-efficient: -0.240, P < 0.01 and positively with GIP and visfatin (Spearman's correlation co-efficient: 0.255 and 0.241, respectively, P < 0.01. The results demonstrate that chronic periodontitis adversely influences the GCF levels of glucoregulatory biomarkers, as it is associated with disturbed levels of biomarkers related to the onset of T2DM and its medical complications.

  15. Impact of Chronic Periodontitis on Levels of Glucoregulatory Biomarkers in Gingival Crevicular Fluid of Adults with and without Type 2 Diabetes.

    Science.gov (United States)

    Mohamed, Hasaan G; Idris, Shaza B; Mustafa, Manal; Ahmed, Mutaz F; Åstrøm, Anne N; Mustafa, Kamal; Ibrahim, Salah O

    2015-01-01

    The relationship between diabetes and periodontal disease is bidirectional, but information about the effect of chronic periodontitis on the levels of the glucoregulatory biomarkers locally in gingival crevicular fluid (GCF) is limited. The aim of this study was to compare the levels of 10 glucoregulatory biomarkers in GCF, firstly in subjects with type 2 diabetes (T2DM) presenting with and without chronic periodontitis and secondly, in subjects without diabetes, with and without chronic periodontitis. The material comprised a total of 152 subjects, stratified as: 54 with T2DM and chronic periodontitis (G1), 24 with T2DM (G2), 30 with chronic periodontitis (G3) and 44 without T2DM or periodontitis (G4). The levels of the biomarkers were measured using multiplex biometric immunoassays. Periodontal pocket depths were recorded in mm. Subsets G1 and G2 and subsets G3 and G4 were compared independently. Among T2DM subjects, GIP, GLP-1 and glucagon were significantly up-regulated in G1 compared to G2. Moreover, there were no statistical differences between the two groups regarding C-peptide, insulin, ghrelin, leptin and PAI-1. Comparisons among individuals without T2DM revealed significantly lower amounts of C-peptide and ghrelin in G3 than in G4. The number of sites with pocket depth ≥ 4mm correlated negatively with C-peptide (Spearman's correlation co-efficient: -0.240, P chronic periodontitis adversely influences the GCF levels of glucoregulatory biomarkers, as it is associated with disturbed levels of biomarkers related to the onset of T2DM and its medical complications.

  16. Evaluation of cortisol levels in gingival crevicular fluid and saliva in anxious and non-anxious patients with chronic periodontitis

    Directory of Open Access Journals (Sweden)

    Sangeeta Umesh Nayak

    2013-01-01

    State - Trait anxiety inventory and Hamilton Anxiety rating scale were used to assess the anxiety levels of all the subjects. Clinical measures such as plaque index (PI, gingival index (GI, probing pocket depth (PPD and clinical attachment level (CAL were recorded. GCF and unstimulated whole saliva samples were collected, and cortisol levels were determined using ELISA kit. Results: PI, GI, PPD, and CAL were higher in Group 3. Hormone level was significantly higher in Group 3. A positive correlation was found among salivary and GCF cortisol and CAL in Group 3. Conclusion: Based on the obtained results individuals with high-levels of anxiety seem to be more prone to have periodontal disease. Salivary and GCF cortisol levels can be used as biomarker for evaluating part of the etiopathogenesis of chronic periodontitis.

  17. [Detection of human papillomavirus in gingival fluid of Venezuelan HIV patients with periodontal disease].

    Science.gov (United States)

    Escalona, Laura; Correnti, María; Veitía, Dayahindira; Perrone, Marianella

    2011-09-01

    Evidence suggests that viruses may be involved in the activation of periodontal disease, allowing the overgrowth of periodontal pathogens. The purpose of the present study was to detect the presence of Human Papillomavirus (HPV) in gingival crevicular fluid (GCF) in HIV+ Venezuelan patients with periodontal disease. We evaluated GCF samples from 20 HIV+ patients with periodontal disease from the Infectious Disease Center, Faculty of Dentistry, Central University of Venezuela, and were clinically examined to establish their periodontal conditions, 13 under HAART (antiretroviral therapy) and 7 without HAART. Seven seronegative patients with chronic periodontitis and 7 seronegative patients, without periodontal disease were included. DNA extraction was performed, the consensus primers MY09 and MY11 for the HPV L1 region were used for PCR amplification. Genotipification was made for the 6, 11, 16, 18, 31 and 45 genotypes. HPV were detected in 46% of HIV+ patients under therapy. The CD4 cell counts in the IIPV+ patients were not significantly different from the HPV-group. The viral load in the HPV+ group was significantly higher (200,470 +/- 324,244 copy/mL) than in the HPV-patients (10,246 +/- 23,805 copy/mL). Genotypes 6 and 11 were observed in the HPV positive samples, of which 4/6 (66.6%) presented coinfection with both types. No significant differences in the periodontal conditions were observed between patients with IIPV-HIV infection related to patients with only HIV. HPV was detected only in the gingival crevicular fluid of HIV+ patients under HAART independently of the periodontal conditions.

  18. 龈沟液钙结合蛋白和总蛋白在实验性龈炎中的变化及相互关系%Calprotectin and total protein in gingival crevicular fluid during experimental gingivitis

    Institute of Scientific and Technical Information of China (English)

    赵亦兵; 孟焕新; 陈智滨

    2003-01-01

    目的:研究健康牙龈向实验性龈炎发展过程中龈沟液(gingival crevicular fluid,GCF)钙结合蛋白和总蛋白水平的变化,探讨钙结合蛋白与牙龈炎之间的关系及其作为牙周炎症标志物的可能性.方法:选取11名牙龈健康、无全身疾病的受试者,在其停止口腔卫生措施后21 d内(第0、7、14、21天)和恢复口腔卫生措施一周后(第28 天)用滤纸条收集其GCF样本(每人4颗牙).分别采用染料结合比色法和ELISA法测定GCF中总蛋白含量和钙结合蛋白含量.结果:在实验性龈炎形成过程中,钙结合蛋白含量逐渐升高,并且在实验第21天达到高峰,恢复口腔卫生措施后其值降低,接近基线水平;总蛋白含量的变化与钙结合蛋白的变化相似.结论:GCF钙结合蛋白含量和总蛋白含量可以较好地反映牙龈炎症的变化.

  19. Dynamic changes of aspartate transaminase level in gingival crevicular fluid on tooth movement in new bone area%犬牙移入牵张成骨区龈沟液 AST 水平动态变化

    Institute of Scientific and Technical Information of China (English)

    凌宁; 王银龙; 李峥

    2015-01-01

    Objective To observe the dynamic changes of aspartate transaminase(AST ) in gingival crevicular fluid on tooth movement in new bone area after distraction osteogenesis at different time .Methods The distraction osteogenesis surgical proce‐dure was performed in 8 beagle dogs without periodontal disease and normal teeth ,experimental teeth were transplanted into the bone regeneration area after 2 weeks and after 6 weeks .Comparative analysis AST of each time (1 ,2 ,3 ,7 ,14 ,28 d after distraction) dynamic changes in gingival crevicular fluid .Results The AST level of gingival crevicular fluid in experimental tooth was rising for the first three days ,and the group of two weeks were significantly higher than 6 weeks ;AST levels after 7 d showed a trend of de‐cline ,down to the lowest point at 21 d ,and gradually restored ,AST levels reached a higher level again in the 28 d .Conclusion The AST level of experimental teeth increased significantly after 2 weeks than after 6 weeks ,but over time the AST level change is not linear ,this change has certain guiding significance for the clinical research in the future .%目的:研究分析不同时机将犬牙移入牵张成骨区后龈沟液的天门冬氨酸氨基转移酶(AST )水平变化。方法8只无牙周疾病且正常恒牙列beag le犬行牵张成骨术后分为两组(2周和6周),均行牙齿移入牵张成骨新骨区。观察对实验牙加力1、2、3、7、14、28 d后龈沟液中AST水平变化。结果两组实验牙龈沟液AST水平前3 d均存在上升趋势,且2周组上升较6周组明显;7 d后AST水平呈现下降趋势,在21 d达最低,并逐渐恢复;当28 d时AST水平再次上升。结论牵张成骨2周后实验牙移入新骨区龈沟液AST整体水平较6周后明显升高,但随着时间的推移AST水平改变并非呈线性升高。

  20. Efecto antiinflamatorio de apitoxina de Apis mellifera sobre prostaglandina E2 del fluido crevicular gingival de pacientes con y sin enfermedad periodontal, sometidos a apiterapia: ensayo preliminar Anti-inflammatory effect of apitoxin and Apis mellifera on prostaglandin E2 in gingival crevicular fluid of patients with and without periodontal disease, submitted to apitherapy: preliminary test

    Directory of Open Access Journals (Sweden)

    W Faúndez Poblete

    2011-08-01

    Full Text Available Introducción: La apitoxina que es producida por la Apis mellifera posee efecto antiinflamatorio sobre una serie de marcadores biológicos. La prostaglandina E2 forma parte de ellos, estando presente en el fluido gingival crevicular (FGC. La prostaglandina E2 es evidenciada en la enfermedad periodontal. Objetivo: En este estudio se evaluó el efecto antiinflamatorio de la apitoxina sobre la concentración de prostaglandina E2 del FGC de un paciente sin enfermedad periodontal (SEP y otro con enfermedad periodontal (CEP. Materiales y Método: Se seleccionó un paciente SEP y otro CEP, que sometidos a apiterapia durante 28 días, se registraron 5 muestras por paciente de FGC, siendo almacenadas, centrifugadas y refrigeradas para su conservación. Posteriormente se midió la concentración de prostaglandina E2 crevicular mediante inmunodifusión radial simple en placas petri con concentración de anticuerpo anti prostaglandina E2 de 1:1000. Selladas a 4°C, se esperó 72 horas para permitir su difusión, tiñéndose con Azul brillante de Coomasie, determinándose la concentración de cada placa. Resultados: Paciente SEP inmediatamente antes de apiterapia presentó una concentración de 0.9636 ± 0.0055 (µg/µL, finalizando con una concentración de 0.9196±0.0733 (µg/µL al completar 28 días de tratamiento. El paciente CEP antes de recibir apiterapia presento una concentración de 1.1866 ± 0.0867 (µg/µL, finalizando con una concentración de 0.9858 ± 0.0074 (µg/µL al completar 28 días de tratamiento. Discusión: Los hallazgos de este estudio demuestran una disminución de la concentración de PGE2 del FGC tanto para el paciente CEP y SEP sometidos a apiterapia durante 28 días, siendo esta disminución 3.7 veces mayor en el paciente CEP.Introduction: Apitoxin, which is produced by Apis mellifera, has anti-inflammatory effect on a number of biomarkers. Prostaglandin E2 is one of them, being present in gingival crevicular fluid (GCF

  1. Analysis of matrix metalloproteinase-8 levels in gingival crevicular fluid and whole mouth fluid among smokers and nonsmokers using enzyme-linked immune-sorbent assay and a novel chair-side test

    Directory of Open Access Journals (Sweden)

    Ghousia Akbari

    2015-01-01

    Full Text Available Aim: To indigenously prepare a chair-side test kit for investigating and comparing the matrix metalloproteinase (MMP-8 levels in gingival crevicular fluid (GCF and saliva in patients with healthy periodontium, gingivitis and chronic periodontitis in smokers and nonsmokers. To validate the diagnostic accuracy of indigenously prepared chair-side test against enzyme-linked immune-sorbent assay (ELISA. Furthermore, to assess the effect of nonsurgical periodontal therapy (NSPT on the levels of MMP-8 in GCF and saliva among the test groups. Materials and Methods: GCF and saliva were collected from 250 subjects. The study population were divided into five groups; health periodontium-nonsmokers (Group 1; n = 50, chronic gingivitis-nonsmokers (Group 2; n = 50, chronic periodontitis-nonsmokers (Group 3; n = 50, chronic gingivitis-smokers (Group 4; n = 50, chronic periodontitis-smokers (Group 5; n = 50. A chair-side test kit was indigenously prepared using polyclonal antibodies (principle of immunochromatography to detect the MMP-8 levels, and it was validated against ELISA at baseline and 3 months after NSPT.Results: The chair-side test detected MMP-8 levels with a sensitivity and specificity in accordance with ELISA. MMP-8 levels at baseline were higher in Group 2 and Group 3 as compared to controls (P < 0.05, and decreased after therapy (P < 0.05. MMP-8 levels in GCF were greater than in saliva for all the groups, indicating GCF to be a better sample to detect the MMP levels.Conclusion: The chair-side test detected MMP-8 levels accurately making it a viable chair side diagnostic tool. It was effective for early diagnosis of the periodontal disease among high-risk population such as smokers.

  2. Lysophosphatidic acid (LPA 18:1 transcriptional regulation of primary human gingival fibroblasts

    Directory of Open Access Journals (Sweden)

    D. Roselyn Cerutis

    2014-12-01

    Full Text Available The pleiotropic, bioactive lipid lysophosphatidic acid [(LPA, 1-acyl-sn-glycerol-3-phosphate] exerts critical regulatory actions in physiology and pathophysiology in many systems. It is present in normal bodily fluids, and is elevated in pathology (1. In vivo, “LPA” exists as distinct molecular species, each having a single fatty acid of varying chain length and degree of unsaturation covalently attached to the glycerol backbone via an acyl, alkyl, or alkenyl link. These species differ in affinities for the individual LPA receptors [(LPARs, LPA1-6] and coupling to G proteins (2. However, LPA 18:1 has been and continues to be the most commonly utilized species in reported studies. The actions of “LPA” remain poorly defined in oral biology and pathophysiology. Our laboratory has addressed this knowledge gap by studying in vitro the actions of the major human salivary LPA species [18:1, 18:0, and 16:0 (3] in human oral cells (4–7. This includes gingival fibroblasts (GF, which our flow cytometry data from multiple donors found that they express LPA1-5 (6. We have also reported that these species are ten-fold elevated to pharmacologic levels in the saliva and gingival crevicular fluid obtained from patients with moderate–severe periodontitis (8. As the potential of LPA to regulate transcriptional activity had not been examined in the oral system, this study used whole human genome microarray analysis to test the hypothesis that LPA 18:1-treated human GF would show significant changes in gene transcripts relevant to their biology, wound-healing, and inflammatory responses. LPA 18:1 was found to significantly regulate a large, complex set of genes critical to GF biology in these categories and to periodontal disease. The raw data has been deposited at NCBI's GEO database as record GSE57496.

  3. 牙周炎对2型糖尿病患者血清和龈沟液IL-6含量影响的研究%The Effect of Periodontitis on the Concentration of IL-6 in Serum and Gingival Crevicular Fluid in Type-2 Diabetic Patients

    Institute of Scientific and Technical Information of China (English)

    王妙妍; 阮毅; 陈广盛

    2014-01-01

    Objective: To detect the effect of Periodontitis on the concentration of IL-6 in serum and gingival crevicular fluid in type 2 diabetic patients.Method: Twenty patients with type-2 diabetes and periodontitis, 20 with simple type-2 diabetes, 20 with simple periodontitis and 16 healthy patients were recruited in this study. The concentration of IL-6 in serum and gingival crevicular fluid were detected by Enzyme Linked Immunosorbent Assay (ELISA), the difference of IL-6 content in serum and gingival crevicular fluid were compared among groups, the correlation between the concentration of IL-6 in serum and that in gingival crevicular fluid were analyzed within group.Result: The concentration of IL-6 in serum and gingival crevicular fluid in three unhealthy groups were significantly higher than that in the healthy group (P<0.001).The concentration of IL-6 in serum and gingival crevicular fluid of the patients with type-2 diabetes and periodontitis were higher than that of the patients just with diabetes or periodontitis. There was a positive correlation between the concentration of IL-6 in serum and that in gingival crevicular fluid respectively in type-2 diabetes patients with periodontitis group (r=0.829,P<0.01), simple diabetes mellitus group (r=0.766,P<0.01) and simple periodontitis group (r=0.732,P<0.01).Conclusion: The concentration of IL-6 in serum and gingival crevicular fluid in type-2 diabetes patients can be increased by periodontitis, IL-6 may play an important role in the interaction between periodontal disease and diabetes.%目的:探讨牙周炎对2型糖尿病患者血清和龈沟液IL-6含量的影响。方法:采用酶联免疫吸附试验(ELISA)检测20例2型糖尿病伴牙周炎患者、20例单纯2型糖尿病患者、20例单纯牙周炎患者及16例健康者的血清和龈沟液(GCF)中IL-6含量,比较组间血清和龈沟液IL-6含量的差异性,分析组内血清和龈沟液IL-6含量的相关性。结果:2型糖

  4. Assessment of the alkaline phosphatase level in gingival crevicular fluid, as a biomarker to evaluate the effect of scaling and root planing on chronic periodontitis: An in vivo study

    Science.gov (United States)

    Kunjappu, Jimly James; Mathew, Vinod Babu; Hegde, Shashikanth; Kashyap, Rajesh; Hosadurga, Rajesh

    2012-01-01

    Context: Clinical evaluation of gingivitis and/or periodontitis does not predict the progression or remission of the disease. Due to this diagnostic constraint, clinicians assume that the pathology has an increased risk of progression and plan treatments, despite the knowledge that all inflamed sites are not necessarily progressing. Extensive research has been carried out on gingival crevicular fluid (GCF) components that might serve as potential diagnostic markers for periodontitis. Among them alkaline phosphatase (ALP) levels in GCF has shown promise as a diagnostic marker. Aim: This study compares the levels of GCF alkaline phosphatase in patients with chronic periodontitis before and after scaling and root planing. Materials and Methods: This study is an in vivo longitudinal study conducted on twenty patients with localized periodontitis. The GCF was collected from the affected site prior to scaling and root planing and ALP level estimated. The probing depth and plaque index at the site were also measured for correlation. Patients were recalled after 7, 30, and 60 days for reassessment. Results: The GCF ALP values showed a sustained, statistically significant decrease after treatment. There was a positive correlation with probing depth but not with plaque index measured at each interval. Conclusion: The assessment of level of periodontal disease and effect of mechanical plaque control on the progression and regression of the disease can be evaluated precisely by the corresponding GCF ALP levels. Thus, alkaline phosphatase level is not only a biomarker for the pathology but also an indicator of prognosis of periodontitis. PMID:22438644

  5. 正常人、牙龈炎和牙周炎患者龈沟液内一氧化氮含量的检测%Detection of the Level of Nitric Oxide in Gingival Crevicular Fluid in Healthy Individuals, Gingivitis and Chronic Periodontitis Patients.

    Institute of Scientific and Technical Information of China (English)

    龚斌; 徐静舒; 戚慧; 雷雅燕

    2011-01-01

    Objective: To observe the level of nitric oxide in gingival crevicular fluid (GCF) in normal group and periodontitis group, and to study the possible role of NO in the periodontal disease. Methods: The GCF samples were collected in 20 periodontally healthy individuals, 22 gingivitis and 32 chronic periodontitis patients. The level of nitric oxide in gingival crevicular fluid was measured by immunofluorescence. Results: The nitric oxide in GCF was a significant difference in chronic periodontitis group and gingivitis group than that in control group(P<0.01). The nitric oxide in GCF was significantly different in chronic periodontitis group compared to that in gingivitis group (P<0.01). Conclusion: Nitric oxide exists in GCF of patients with chronic periodontitis and gingivitis and periodontally healthy individuals. Nitric oxide plays an important role in the development of chronic periodontal disease. The level of nitric oxide in GCF has close relation with degree of inflammation.%目的:检测正常人和牙周病患者龈沟液中NO含量,探讨NO在牙周病发病过程中的作用.方法:选择牙周健康组20例,牙龈炎组22例,慢性牙周炎组32例,分别采集龈沟液标本,免疫荧光法检测龈沟液内NO的含量.结果:慢性牙周炎患者和牙龈炎患者龈沟液内NO含量与牙周健康组相比均有高度显著性差异(P<0.01),慢性牙周炎患者龈沟液内N0含量与牙龈炎组相比有高度显著性差异(P<0.01).结论:牙周健康者、牙龈炎患者、慢性牙周炎患者龈沟液中能检测出N0的存在,NO参与了慢性牙周炎的发展过程,龈沟液内NO含量与慢性牙周炎炎症程度密切相关.

  6. Concentrations of MMP-2 in gingival crevicular fluid of porcelain teeth with three different alloys%三种金属烤瓷冠修复后龈沟液中MMP-2水平检测

    Institute of Scientific and Technical Information of China (English)

    许卫星; 苏俭生; 袁剑鸣

    2012-01-01

    目的 研究镍铬合金、钴铬合金、金合金三组金属烤瓷冠修复前后,龈沟液(gingival crevicular fluid,GCF)中基质金属蛋白酶-2(matrix metalloproteinase,MMP-2)水平变化,提示三种合金烤瓷冠在不同时期对牙周组织的刺激程度.方法 临床随机选择三种金属烤瓷冠修复患者共45例,每组各15例,测定在修复前、修复后1个月、3个月、6个月GCF量并采用双抗体夹心酶联免疫吸附法(ABC-ELISA)检测同期GCF中MMP-2水平.结果 镍铬合金烤瓷冠GCF量、MMP-2水平在不同检测周期差异有显著性(P<0.01),钴铬合金、金合金烤瓷冠修复后MMP-2水平在不同检测周期差异无显著性(P>0.05).结论 MMP-2可作为对不同金属烤瓷冠内冠材料刺激牙周组织的评价指标,临床应采用生物相容性好的合金作为内冠材料.%Objective To measure MMP-2 concentrations in gingival crevicular fluid ( GCF) of porcelain teeth coated with Ni-Cr, Co-Cr or gold alloy at different time period, and to demonstrate the degree of stimulation by these alloy on gingivae. Methods Sandwich enzyme-linked immunosorbent assay(ELISA) was used to determine the concentration and total amount of MMP-2 in gingival crevicular fluids in 45 randomly selected cases of porcelain teeth coated with Ni-Cr, Co-Cr or gold alloy, with 15 cases in each group. The assay was performed before and at 1, 3, 6 months after treatment. Results There was significant difference in the total amounts of MMP-2 and GCF volume for the Ni-Cr alloy coated porcelain teeth in different time period(P 0. 05 ). Conclusion MMP-2 is a useful assessment criterion for the evaluation of degree of stimulation on gingivae by different alloy coating materials in porcelain teeth. The bio-compatibility of alloy coating for porcelain teeth should be carefully considered for the clinical application.

  7. Granulocyte elastase levels in saliva and gingival crevicular fluid of subjects with various periodontal conditions%不同牙周状况下唾液和龈沟液中弹性蛋白酶的含量

    Institute of Scientific and Technical Information of China (English)

    康军; 沙月琴; 陈智滨

    2012-01-01

    Objective: To compare the granulocyte elastase ( EA) levels in saliva and/or gingival cre-vicular fluild (GCF) of subjects with various periodontal conditions and analyze the relation between EA levels in GCF and in saliva. Methods; GCF and salivary samples were collected from 17 subjects with healthy periodontium, 14 with gingivitis, 24 with chronic periodontitis ( CP) and 24 with aggressive periodontitis (AgP). The EA levels in GCF and saliva were analyzed. Results; The GCF-EA level in AgP were significantly higher than that in CP (0.485 3 ±0.225 0 vs. 0.2884±0.193 1, P<0.01); the levels of EA in saliva of periodontitis patients ( AgP and CP) were higher than those of healthy and gingivitis subjects (0. 844 5 ±0.660 6, 0.637 3 ±0.648 9 vs. 0.031 6 ±0.020 6, 0.012 2 ±0.005 8, P < 0. 001). A positive correlation was found between EA levels in saliva and those in GCF ( r -0. 660) . Conclusion: GCF-EA level may serve as a marker for clinical assessment of periodontal conditions. The measurement of EA levels in saliva may facilitate to overall screen periodontitis patients in epi-demiological study or to monitor periodontal conditions in clinical practice.%目的:比较不同牙周状况者唾液和龈沟液( gingival crevicular fluild,GCF)中的中性多形核粒细胞弹性蛋白酶( granulocyte elastase,EA)含量,并分析唾液与龈沟液EA水平的相关性.方法:采用底物法对牙周健康者(17例)、牙龈炎患者(14例)、慢性牙周炎( chronic periodontitis,CP)患者(24例)和侵袭性牙周炎(aggressive periodontitis,AgP)患者(24例)的唾液和/或龈沟液样本中的EA含量进行测定分析.结果:AgP患者龈沟液EA水平高于CP患者(0.485 3 ±0.225 0 vs.0.288 4±0.193 1,P<O.01);牙周炎患者(AgP和CP)唾液EA水平高于牙周健康者和牙龈炎患者(0.844 5±0.660 6,0.637 3±0.648 9 vs.0.012 2±0.005 8,0.031 6±0.020 6;P均<0.001).牙周炎患者唾液与龈沟液EA水平呈正相关关系(r=0.660).结论:龈沟液EA

  8. A Sensitive Method for the Detection of Immune Complexes in Human Gingival Crevicular Fluid

    Science.gov (United States)

    1993-01-01

    Report) This document has been approved for public release; distribution unlimited. 18. SUPPLEMENTARY NOTES Journal of Clinical Periodontology 1992; 19...Northwestern University but with intervals of 10, 10, 15, 20, and dontitis, deposits of ICs and comple- Dental School Clinic provided the GCF 30 min...Nachweismie- of antibody. Journal of Pt-riodontal Re- :tmcter inl a profile to indicate active thode karno zur F rkundung dcr Fragestellung Mearri/ 19

  9. 种植体周围龈沟液中硫离子含量的测定及其临床意义%Determination of Sulfide Levels in Gingival Crevicular Fluid around Dental Implants and Its Clinical Significance

    Institute of Scientific and Technical Information of China (English)

    李明; 逄爱慧; 杨焰; 吴丽娟; 马净植

    2013-01-01

    Objective To evaluate the correlation between sulfide level in gingival crevicular fluid around dental implants and the peri-implant parameters ,and to explore the feasibility of using SUL as a peri-implant monitoring index. Methods Three groups were designed ,including peri-implant mucositis (PIM ) group (47 implants ) ,peri-implantitis (PI) group (26 implants) and normal control group (50 implants). Six sites were randomly selected around the dental implants. The sulfide level in the gingival crevicular fluid at the selected sites was measured by using Diamond Probe /Perio 2000 System as well as the peri-implant clinical parameters such as sulcus bleeding index (SBI),probing pocket depth (PD ) and attachment loss (AL ). Results There were significant differences in the sulfide level among the three groups [(0. 355 9±0. 039 0) pg/mL in PI group ,(0. 125 3± 0. 037 1) pg/mL in PIM group ,(0. 052 8±0. 017 3 ) pg/mL in control group](P<0. 01 ). The sulfide level was increased with the severity of the peri-implant lesion. It was positively correlated with such peri-implant parameters as PLI (r= 0. 733),SBI (r=0. 859),PD 0=0. 873) and AL(r= 0.606) ( P<0. 01 ). Conclusion The sulfide level in the gingival crevicular fluid a-round dental implants was significantly correlated with the clinical peri-implant indices,suggesting that the sulfide level may be used to reflect the status of peri-implant tissues and the lesion progression in clinical practice.%目的 分析种植体周围龈沟液(gingival crevicular fluid,GCF)中硫化物水平(sulfide levels,SUL)与种植体牙周指数之间的相关性,探讨SUL作为种植体牙周监测指标的可行性.方法 设种植体周围黏膜炎组(peri-implant mucositis,PIM)47例(47颗牙位);种植体周围炎组(peri-implantitis,PI)26例(26颗牙位)和健康种植牙对照组50例(50颗牙位).每个牙位记录6个位点,采用金刚牙周诊断仪测量所选位点GCF中SUL和种植体牙周临床指标:龈

  10. Detection and Clinical Benefits of Interleukin-1β in Gingival Crevicular Fluid in the patients with Periodontitis%牙周炎患者龈沟液白细胞介素-1β检测及意义

    Institute of Scientific and Technical Information of China (English)

    莫弼凡; 钱成明

    2001-01-01

    目的:检测牙周炎患者龈沟液(gingival crevicular fluid, GCF)中白细胞介素-1β(interleukin-1β, IL-1β)的含量变化,探讨 IL-1β在牙周炎发病中的作用.方法:用滤纸条浸润法采集正常成年人及成年人牙周炎患者治疗前后 GCF样本,用酶联免疫吸附法( ELISA)检测 GCF中 IL-1β含量变化.结果:牙周炎患者龈沟液中 IL-1β水平显著高于正常对照组(P<0.01),牙周炎治疗组患者龈沟液中 IL-1β水平较牙周炎组显著降低(P<0.01).龈沟液中 IL-1β水平分别与牙龈指数、探诊出血指数、牙周袋深呈显著正相关.结论:IL-1β参与牙周炎的发生和发展, GCF中 IL-1β可作为检查牙周病活动情况的一项较为敏感的指标.

  11. Aggregatibacter actinomycetemcomitans lipopolysaccharide affects human gingival fibroblast cytoskeletal organization.

    Science.gov (United States)

    Gutiérrez-Venegas, Gloria; Contreras-Marmolejo, Luis Arturo; Román-Alvárez, Patricia; Barajas-Torres, Carolina

    2008-04-01

    The cytoskeleton is a dynamic structure that plays a key role in maintaining cell morphology and function. This study investigates the effect of bacterial wall lipopolysaccharide (LPS), a strong inflammatory agent, on the dynamics and organization of actin, tubulin, vimentin, and vinculin proteins in human gingival fibroblasts (HGF). A time-dependent study showed a noticeable change in actin architecture after 1.5 h of incubation with LPS (1 microg/ml) with the formation of orthogonal fibers and further accumulation of actin filament at the cell periphery by 24 h. When 0.01-10 microg/ml of LPS was added to human gingival fibroblast cultures, cells acquired a round, flat shape and gradually developed cytoplasmic ruffling. Lipopolysaccharides extracted from Aggregatibacter actinomycetemcomitans periodontopathogenic bacteria promoted alterations in F-actin stress fibres of human gingival cells. Normally, human gingival cells have F-actin fibres that are organized in linear distribution throughout the cells, extending along the cell's length. LPS-treated cells exhibited changes in cytoskeletal protein organization, and F-actin was reorganized by the formation of bundles underneath and parallel to the cell membrane. We also found the reorganization of the vimentin network into vimentin bundling after 1.5 h of treatment. HGF cells exhibited diffuse and granular gamma-tubulin stain. There was no change in LPS-treated HGF. However, vinculin plaques distributed in the cell body diminished after LPS treatment. We conclude that the dynamic and structured organization of cytoskeletal filaments and actin assembly in human gingival fibroblasts is altered by LPS treatment and is accompanied by a decrease in F-actin pools.

  12. 盐酸米诺环素对慢性牙周炎基质金属蛋白酶-8水平的影响%Effect of Minocycline Hydrochloride Ointment on MMP-8 in Gingival Crevicular Fluid Abstract

    Institute of Scientific and Technical Information of China (English)

    井枫秋; 汪黎明; 张洁; 黄云亮

    2011-01-01

    目的 研究盐酸米诺环素对慢性牙周炎患者龈沟液中基质金属蛋白酶-8(matrix metalloproteinase-8,MMP-8)水平的影响.方法 选26例中重度慢性成人牙周炎患者,每例口腔左右两个象限随机分为实验组和对照组.基础治疗后实验组使用盐酸米诺环素缓释软膏,每周1次,共2次.对照组不使用盐酸米诺环素软膏,仅使用生理盐水和10%过氧化氢冲洗.使用酶联免疫法(ELISA)测定两组治疗前后龈沟液中基质金属蛋白酶-8的水平.结果 实验组、对照组在基础治疗后龈沟液中基质金属蛋白酶-8总量和浓度均明显降低(P<0.01).实验组与对照组龈沟液中基质金属蛋白酶-8总量和浓度的变化差异有统计学意义,实验组基质金属蛋白酶-8总量和浓度减少的量要多于对照组(P<0.01).结论 基础治疗和盐酸米诺环素缓释软膏联合应用能有效降低慢性牙周炎患者龈沟液中基质金属蛋白酶-8水平,改善牙周状况.%Objective To analyze the changes of matrix metalloproteinases - 8 ( MMP - 8) levels in gingival crevicular fluid (GCF) after subgingivally administering 2% minocycline - HCl ointment to the periodontal pocket as an auxiliary treatment of moderate and severe chronic periodontitis. Methods Twenty - six subjects were selected. The left and right sides of oral cavity were divided into control group and test group with a randomized single blind method. After initial periodontal treatment of both groups, the test group was treated with the sustained release unguent of minocycline - HCL ointment in the pocket once a week for 2 weeks,while the control group with 0.9 % normal saline and 10% hydrogen peroxide. Two weeks later, gingival crevicular fluid samples were collected and detected with ELISA technique. Results The level of MMP - 8 in GCF obviously declined after treatment in both groups( P <0.01 ), particularly in the test group. The difference was statistically sigificant(P < 0. 01

  13. INFLUENCE OF LACTATE DEHYDROGENASE ISOENZYME IN GINGIVAL CREVICULAR FLUID AFTER NI-CR ALLOY DENTAL RESTORATION%镍铬合金修复体对龈沟液中LDH同工酶谱的影响

    Institute of Scientific and Technical Information of China (English)

    周俊红; 周立社; 秦文斌; 雎天林; 李利

    2016-01-01

    目的:探讨镍铬合金修复牙体后龈沟液( gingival crevicular fluid, GCF )中乳酸脱氢酶( lactate dehydrogenase,LDH)同工酶的变化。方法:选择镍铬合金修复1~2a的牙位58个,健康牙位54个,用滤纸条法采集GCF,醋酸纤维薄膜电泳结合光密度扫描法测定GCF-LDH同工酶的相对含量。结果:金属修复牙组与健康对照组比较,两组间GCF中LDH1、LDH2、LDH3和LDH5的相对含量均无显著性差异(t1、t2、t3、t5分别为0.49、1.56、0.12、0.37;P>0.05),但镍铬合金修复组GCF-LDH4相对含量明显高于健康组(t4=2.03,P40. 05 ), but the GCF-LDH4 content in the prothetic teeth group was significantly higher than it in the healthy teeth group(t4=2. 03,P4<0. 05). Conclusion:The GCF-LDH isoenzyme-gram of Ni-Cr alloy prothetic teeth is changed. It indicates that Ni-Cr alloy dental prosthesis is likely to have a stimulation or injury to periodontium.

  14. Gingival crevicular fluid, serum levels of receptor activator of nuclear factor-κB ligand, osteoprotegerin, and interleukin-17 in patients with rheumatoid arthritis and osteoporosis and with periodontal disease.

    Science.gov (United States)

    Gümüş, Pınar; Buduneli, Eralp; Bıyıkoğlu, Başak; Aksu, Kenan; Saraç, Fulden; Nile, Christopher; Lappin, David; Buduneli, Nurcan

    2013-11-01

    This study is performed to evaluate gingival crevicular fluid (GCF) and serum levels of soluble receptor activator of nuclear factor-κB ligand (sRANKL), interleukin (IL)-17A, IL-17E, IL-17F, IL-17A/F, and osteoprotegerin (OPG) in women with rheumatoid arthritis (RA), osteoporosis (OPR), and those who are systemically healthy (SH), all with periodontal disease. GCF and serum samples were obtained before any periodontal intervention from 17 women with RA, 19 with OPR, and 13 who were SH with periodontitis. Full-mouth clinical periodontal measurements were recorded. sRANKL, OPG, and IL-17 levels were determined by enzyme-linked immunosorbent assay. Clinical periodontal measurements were similar in the three study groups. Although the total amounts of GCF albumin, OPG, IL-17A, and IL-17A/F were similar in the study groups, there were statistically significant differences in GCF concentrations of sRANKL, OPG, IL-17A, IL-17E, IL-17F, and IL-17A/F. The sRANKL/OPG ratios were significantly higher in the RA group than in the OPR and SH groups (P <0.05). Serum sRANKL, sRANKL/OPG, and IL-17A/IL-17E ratios were significantly higher, whereas OPG concentrations were significantly lower in the RA group compared to other groups (P <0.05). Serum IL-17A concentrations were significantly higher in the RA and OPR groups than in the SH group (P <0.05). Increased inflammatory mediator levels in patients with RA, despite the long-term use of various anti-inflammatory drugs, suggest that these patients may have a propensity to overproduce these inflammatory mediators.

  15. 引导组织再生术的应用体会及龈沟液的检测意义%Significance of determining gingival crevicular fluid volume in guided tissue regeneration

    Institute of Scientific and Technical Information of China (English)

    李凤莉; 吴小芳

    2011-01-01

    目的:评价引导组织再生术在垂直性骨缺损治疗中的临床效果及龈沟液的检测意义.方法:选取60例垂直性骨缺损牙周病患牙随机分为两组,A组为引导组织再生术组( GTR),植入Bio-oss多孔骨后覆盖Bio-gide胶原膜;B组为对照组(OFD),行常规翻瓣术.术前、术后3、6、12个月观察牙周袋深度(PD)、附着丧失(AL)、牙松动度(TM)、龈沟液(GCF)量及X线片牙槽骨的变化.结果:术后3个月两组各项均较术前减少,A组X线片骨高度和密度轻度增加.术后12个月A组较B组PD、AL、TM及X线片骨高度和密度差异均有显著性,两组GCF无明显差异.结论:引导组织再生术在垂直性骨缺损治疗中的临床效果良好,Bio-oss多孔骨和Bio-gide胶原膜能有效恢复牙周垂直性骨缺损,改善临床指标,促进牙周新附着的形成;龈沟液流量可作为术后炎症的观测指标.%Objective:To observe the clinical effects of guided tissue regeneration ( GTR) in treatment of the vertical bone defect with determination of gmgival crevicular fluid. Methods: Sixty cases with intrabony per-iodontal bone defects were randomized into two groups. Croup A received treatment of GTR with graft of deproteinized bovine bone mineral ( Bio-oas) and collagen barrier membrane( Bio-gide) and group B.the control group, were treated by open-flap debridement ( OFD ) . The two groups were observed before the operation and at the 3rd ,6th and 12th month after the procedure regarding the periodonlal pocket depth ( PPD), attachment toss ( AL) .tooth mobility ( TM ) ,gingival crevicular fluid ( GCF) volume and the defects of alveolar bone on radiograms. Results: By three months of therapy, the four parameters were reduced as compared with pre-operation, and the alveolar bone height and density on radiograms were increased slightly in group A, By the 12th month after operation, PPD, AL,TM and the alveolar bone height and density on radiograms were significantly

  16. Cytokines in crevicular fluid and orthodontic tooth movement

    NARCIS (Netherlands)

    Ren, Yijin; Vissink, Arjan

    2008-01-01

    This review aimed to evaluate studies on cytokines in the gingival crevicular fluid (GCF) during orthodontic treatment, summarizing the regulation patterns of the most commonly studied cytokines and exploring their clinical implications. To achieve this, a number of key databases were searched using

  17. Cytokines in crevicular fluid and orthodontic tooth movement

    NARCIS (Netherlands)

    Ren, Yijin; Vissink, Arjan

    This review aimed to evaluate studies on cytokines in the gingival crevicular fluid (GCF) during orthodontic treatment, summarizing the regulation patterns of the most commonly studied cytokines and exploring their clinical implications. To achieve this, a number of key databases were searched using

  18. Metallothionein in human gingival amalgam tattoos.

    Science.gov (United States)

    Lau, J C; Jackson-Boeters, L; Daley, T D; Wysocki, G P; Cherian, M G

    2001-11-01

    Amalgam tattoos occur when small particles of dental amalgam, composed largely of silver (Ag) and mercury (Hg), are inadvertently implanted into oral soft tissues during dental procedures. Metallothioneins (MTs) are ubiquitous, low molecular weight, cysteine-rich, metal-binding proteins that are inducible by many agents including metals and may be involved in the detoxification of toxic metals such as Hg. In this study, the correlation between MT expression and amalgam tattoos in human gingiva was investigated using energy-dispersive X-ray microanalysis (EDX) and immunohistochemical techniques. Light microscopically, amalgam tattoos presented as either fine granular particles or larger discrete opaque globular particles in connective tissues. EDX revealed the smaller particles to be silver sulphide (Ag(2)S), while the larger particles exhibited a shell of Ag(2)S that contained irregularly distributed masses of Ag and Hg. Particles of tin (Sn) were also found. No MT staining was observed in collagen, fibroblasts or blood vessels in areas exhibiting abundant amounts of embedded fine granular Ag(2)S particles. Blood vessels exhibiting relatively few amalgam particles stained positively for MT. Cells with the morphological features of histiocytes located directly adjacent to larger pieces of amalgam showed intense MT staining. These results indicate that amalgam tattoos contain no Hg or free Ag except in large globular pieces of amalgam, which still contain Hg and which induce MT expression in adjacent histiocytes. This suggests that Hg leaching from impacted dental amalgam particles induces MT, while residual Ag(2)S and Sn particles do not. MT may therefore act to reduce Hg exposure in patients with amalgam tattoos.

  19. Establishment and evaluation of method to collect rat gingival crevicular fluid by using absorbent paper points%大鼠龈沟液吸潮纸尖采集方法的建立及其评价

    Institute of Scientific and Technical Information of China (English)

    左志刚; 胡敏; 刘珊; 李志敏; 姜欢; 李洪发

    2014-01-01

    Objective To establish and evaluate the method to collect the rat gingival crevicular fluid (GCF)by using absorbent paper points, and to lay foundation for analysis on GCF.Methods 20 healthy male rats were selected and randomly divided into GCF group and saliva group.The GCF of the right upper molar gingival trough of the rats in GCF group and the saliva of the rats in saliva group were collected by using 1 5# absorbent paper points.The SDS-PAGE analysis and abundance detection were applied to analyze the protein bands of the samples in two groups.Results The SDS-PAGE analysis identified the proteins at 77 000,66 000,55 000,51 000,and 28 000,especially 66 000 in GCF group.While saliva group had lower brightness protein bands at 66 000,60 000, and 48 000.The data of protein abundance of 66 000 between two groups had statistically significant difference (P<0.05).Conclusion The number and types of the protein bands are different between GCF Group and saliva group,so using 15# absorbent paper points can collect the rat GCF successfully.%目的:建立并评价大鼠龈沟液(GCF)吸潮纸尖采集方法,为大鼠龈沟液分析奠定基础。方法:选取健康雄性大鼠20只,随机分为 GCF组和唾液组,每组各10只。15#吸潮纸尖分别采集 GCF组大鼠右上第一磨牙腭侧龈沟内液体和唾液组大鼠唾液。样本离心后进行 SDS-PAGE分析及蛋白丰度检测,分析2组样本的蛋白成分。结果:GCF组样本电泳结果可见相对分子质量约为77000、66000、55000、51000和28000等的蛋白质条带,尤以66000处条带亮度最高。唾液组样本在相对分子质量约为66000、60000和48000附近隐约可见蛋白质条带,条带亮度较低。2组样本66000条带处蛋白丰度值比较差异有统计学意义(P<0.05)。结论:GCF组样本的蛋白条带数量以及种类不同于唾液组样本,证明本实验利用15#吸潮纸尖袋内多次取样法可排除唾液、血

  20. HEMA but not TEGDMA induces autophagy in human gingival fibroblasts

    Science.gov (United States)

    Teti, Gabriella; Orsini, Giovanna; Salvatore, Viviana; Focaroli, Stefano; Mazzotti, Maria C.; Ruggeri, Alessandra; Mattioli-Belmonte, Monica; Falconi, Mirella

    2015-01-01

    Polymerized resin-based materials are successfully used in restorative dentistry. Despite their growing popularity, one drawback is the release of monomers from the polymerized matrix due to an incomplete polymerization or degradation processes. Released monomers are responsible for several adverse effects in the surrounding biological tissues, inducing high levels of oxidative stress. Reactive oxygen species are important signaling molecules that regulate many signal-trasduction pathways and play critical roles in cell survival, death, and immune defenses. Reactive oxygen species were recently shown to activate autophagy as a mechanism of cell survival and cell death. Although the toxicity induced by dental resin monomers is widely studied, the cellular mechanisms underlying these phenomena are still unknown. The aim of the study was to investigate the behavior of human gingival cells exposed to 2-hydroxy-ethyl methacrylate (HEMA) and triethylene glycol dimethacrylate (TEGDMA) to better elucidate the mechanisms of cell survival and cell death induced by resin monomers. Primary culture of human gingival cells were exposed to 3 mmol/L of HEMA or 3 mmol/L of TEGDMA for 24, 48, and 72 h. Morphological investigations were performed by transmission electron microscopy to analyze the ultrastructure of cells exposed to the monomers. The expression of protein markers for apoptosis (caspase – 3 and PARP) and autophagy (beclin – 1 and LC3B I/II) were analyzed by western blot to investigate the influence of dental resin monomers on mechanisms underlying cell death. Results showed that HEMA treatment clearly induced autophagy followed by apoptosis while the lack of any sign of autophagy activation is observed in HGFs exposed to TEGDMA. These data indicate that cells respond to monomer-induced stress by the differential induction of adaptive mechanisms to maintain cellular homeostasis. PMID:26483703

  1. HEMA but not TEGDMA Induces Autophagy in Human Gingival Fibroblasts.

    Directory of Open Access Journals (Sweden)

    gabriella eteti

    2015-10-01

    Full Text Available Polymerized resin-based materials are successfully used in restorative dentistry. Despite their growing popularity, one drawback is the release of monomers from the polymerized matrix due to an incomplete polymerization or degradation processes. Released monomers are responsible for several adverse effects in the surrounding biological tissues, inducing high levels of oxidative stress. Reactive oxygen species are important signaling molecules that regulate many signal-trasduction pathways and play critical roles in cell survival, death, and immune defenses. Reactive oxygen species were recently shown to activate autophagy as a mechanism of cell survival and cell death. Although the toxicity induced by dental resin monomers is widely studied, the cellular mechanisms underlying these phenomena are still unknown. The aim of the study was to investigate the behavior of human gingival cells exposed to 2-hydroxy-ethyl methacrylate (HEMA and triethylene glycol dimethacrylate (TEGDMA to better elucidate the mechanisms of cell survival and cell death induced by resin monomers. Primary culture of human gingival cells were exposed to 3mmol/L of HEMA or 3mmol/L of TEGDMA for 24 h, 48h, and 72 h. Morphological investigations were performed by transmission electron microscopy to analyze the ultrastructure of cells exposed to the monomers. The expression of protein markers for apoptosis (caspase – 3 and PARP and autophagy (beclin – 1 and LC3B I/II were analyzed by western blot to investigate the influence of dental resin monomers on mechanisms underlying cell death. Results showed that HEMA treatment clearly induced autophagy followed by apoptosis while the lack of any sign of autophagy activation is observed in HGFs exposed to TEGDMA. These data indicate that cells respond to monomer-induced stress by the differential induction of adaptive mechanisms to maintain cellular homeostasis.

  2. The effect of basic periodontal therapy on the tumor necrosis factor-alpha in serum and gingival crevicular fluid in diabetes patients with periodontitis%牙周基础治疗对糖尿病患者血清和龈沟液中肿瘤坏死因子-α的影响

    Institute of Scientific and Technical Information of China (English)

    裴路; 谢晓华; 郭阳; 张瑞敏; 刘枫晨

    2012-01-01

    Objective The aim of this study was to evaluate the effects of basic periodontal therapy on the level of tumor necrosis factor-alpha in serum and gingival crevicular fluid,clinical periodontal measurements,glycemic control in type two diabetic patients (T2DM) with chronic periodontitis.Methods Sixty patients with type two diabetes and chronic periodontitis,randomly divided into basic periodontal therapy group (observation group) and non-basic periodontal therapy group (control group),and each group was 30 patients.All subjects received basic periodontal therapy.Clinical periodontal parameters:probing depth (PD),attachment loss(AL),and plaque index (PLI) were recorded at baseline,1 and 3 months after the treatment,HbA1c and the level of TNF-α in serum and gingival crevicular fluid were measured.Results At 1 and 3 months after treatment,PD,PLI and TNF-α in gingival crevicular fluid significantly reduced (P < 0.05) in observation group,but AL and the concentration of HbAlc and TNF-α in serum reduced significantly 3 months after treatment (P < 0.05),while that of control group had no significant difference after the treatment (P>0.05).Conclusion The basic periodontal therapy can help to control the glucose level,clinical periodontal improvements and decreases TNF-α in serum and gingival crevicular fluid in type two diabetic patients with periodontitis.%目的 观察牙周基础治疗对2型糖尿病伴牙周炎患者血清及龈沟液中肿瘤坏死因子-α(TNF-α)的浓度、临床牙周状态、血糖控制的影响.方法 选取2型糖尿病伴牙周炎患者60例,随机分做牙周基础治疗组(观察组)和不做牙周基础治疗组(对照组),每组各30人.分别在治疗前、治疗后1个月和3个月记录所有患者牙周临床指数:探诊深度(PD),附着丧失(AL)及菌斑指数(PLI),并检测糖化血红蛋白(HbAlc)及血清及龈沟液中TNF-α的含量.结果 观察组中PD、PLI和龈沟液中TNF-α含量在治疗后1个月和3

  3. Observation on the influence of fixed appliance for multi-indexes of gingival crevicular fluid and periodontal status of patients treated with orthodontic treatment%固定矫治器对正畸治疗患者龈沟液检测指标及牙周状况的影响

    Institute of Scientific and Technical Information of China (English)

    程伟; 程名涛; 朱亚妮

    2013-01-01

    目的:探讨固定矫治器对正畸治疗患者龈沟液多个检测指标及牙周状况的影响.方法:选取2009年7月~2012年4月于本院采用固定矫治器进行正畸治疗的38例患者为研究对象,将其正畸治疗前及治疗后1、3、5、8及12周的龈沟液量、龈沟液前列腺素2 (PGE2)、活化激酶5(PAK5)、β-防御素2(HBD-2)、内毒素(ET)、白细胞介素-1 β(IL-1 β)及出血指数(BI)、牙周袋深度(PD)、牙松动度(TM)、菌斑指数(PLI)、牙龈指数(GI)、简化口腔指数(OHI-S)进行检测及比较.结果:38例患者治疗前及治疗后1、3、5、8及12周的龈沟液量、龈沟液PGE2、PAK5、HBD-2、ET、IL-1 β均呈现先升后降的趋势,而BI、PD、TM、PLI、GI、OHI-S则从治疗前及治疗后8周呈现持续升高的趋势(P<0.05),有显著性差异(P<0.05);治疗后12周与8周则无显著性差异(P>0.05).结论:固定矫治器对正畸治疗患者龈沟液多个检测指标及牙周状况的影响较为明显,但至1 2周时基本稳定.%Objective To study the influence of fixed appliance for multi -indexes of gingival crevicular fluid and periodontal status of patients treated with orthodontic treatment. Methods 38 patients treated with fixed appliance in our hospital from July 2009 to April 2012 were selected as research object.and the amount of gingival crevicular fluid, PGE2,PAK5,HBD-2,ET,IL-1P and BI,PD,TM,PLI,GI,OHI-S of all the patients before and after the treatment at first.third, fifth,eighth and twelfth week were detected and compared. Results The gingival crevicular fluid, PGE2,PAK5,HBD-2,ET and IL-1(3of patients increased first and decreased afterwards.and BI,PD,TM,PLI,GI,OHI-S from the pre-treatment to post-treatment at eighth week showed continuously elevated (P0.05). Conclusion The influence of fixed appliance for multi-indexes of gingival crevicular fluid and periodontal status of patients treated with orthodontic treatment is obvious, and those at twelfth week were

  4. Comparative study on inflammatory cytokine levels in gingival crevicular fluid during orthodontic tooth movement with Smart Clip self-ligating brackets and conventional brackets%Smart Clip 自锁托槽和传统托槽对正畸牙龈沟液中炎症因子水平的影响研究

    Institute of Scientific and Technical Information of China (English)

    韩骁; 刘昕; 张晏更; 葛宇

    2014-01-01

    Objective To investigate changes of the cytokine levels in the gingival crevicular fluid after applying force on the self-li-gating brackets and conventional brackets,and analyse the periodontal tissue remodeling.Methods Sixteen maxillary protruding pa-tients who needed removing bilateral maxillary first premolars were selected,self-ligating appliance being used on one side of the upper dentition as the experimental group,and using traditional straight wire appliance on the other side as the control group.IL-1 βand PGE2 levels of the gingival crevicular fluid taken at different time points in canine retraction were detected,and to analyze the relationship of their changes with periodontal tissue inflammatory response.Results After retruding the canines,levels of IL-1 βand PGE2 of the gin-gival crevicular fluid all increased and reached their top at 24 h.There were no significant differencesin IL-1 βand PGE2 levels of the gingival crevicular fluid between the experimental group and control group.Conclusions Self-ligating appliance did not differ signifi-cantly from traditional appliance in periodontal tissue remodeling in the early phase of the orthodontic tooth movement.%目的:探讨自锁矫治器和传统矫治器对牙齿加力后龈沟液中炎症因子水平变化从而比较两种类型托槽引起的牙周组织改建的情况。方法选择16例上颌前突需拔除上颌双侧第一前磨牙的患者,上牙列一侧使用自锁矫治器作为试验组,一侧使用传统直丝弓矫治器作为对照组,对两侧尖牙加力后取不同时间点的龈沟液,检测其中 IL -1β和 PGE2水平,分析其变化与牙周组织炎症反应的关系。结果在对尖牙加力后,龈沟液中 IL -1β和 PGE2水平均升高,均在加力后24 h 达到最高值,各检测时间点试验组和对照组龈沟液中 IL -1β和 PGE2含量差异无统计学意义(P>0.05)。结论2种矫治器在正畸牙移动的初期对牙周组织改建的影响无明显区别。

  5. Detection and clinical benefits of keratinocyte growth factor KGF-1 in gingival crevicular fluid in the patients with periodontitis.%慢性牙周炎龈沟液中KGF-1含量检测及其意义

    Institute of Scientific and Technical Information of China (English)

    赵哲; 武云霞; 孙晓军

    2011-01-01

    目的:检测慢性牙周炎患者牙周基础治疗前及治疗后不同时期龈沟液中角质细胞生长因子(kemtinoeytegrowth factor,KGF)-1的质量浓度,探讨KGF-1与牙周炎的关系及其在牙周炎发病机理、病情进展方面的作用.方法:用滤纸条浸润法采集牙周健康者和慢性牙周炎患者治疗前后不同时期的龈沟液样本,用酶联免疫吸附测定法检测样本中KGF-1的质量浓度.结果:慢性牙周炎患者龈沟液中KGF-1的质量浓度高于健康对照组(P<0.05),牙周基础治疗后第4、6、8周复查,KGF-1的质量浓度低于治疗前,且治疗后不同时期的复查结果与治疗前相比,均有统计学意义(P<0.05),KGF-1的质量浓度与牙周探诊深度(PD)、附着丧失(AL)、龈沟出血指数(SBI)存在正相关.结论:龈沟液中KGF-1水平与牙周炎症密切相关,KGF-1可作为反映牙周状态的客观指标.%Objective: To detect the concentration of keratinocyte growth factor (KGF)-1 in gingival crevicular fluid (GCF) in the patients with chronic periodontitis before treatment and in different periods after treatment,to research the relationship between KGF-1 in GCF and periodontitis and the effect of KGF-1 in pathogenesis of periodontitis and its progressiveness. Method:The concentration of KGF-1 in GCF were detected by way of enzyme-linked immunosorbent assay following careful sampling of GCF with the standardized filter strips in healthy adults as well as periodontitis patients before treatment and in different stages after treatment. Result:The concentration of KGF-1 in GCF in the inflammatory group was significant higher than the normal group (P <0.05),at the fourth week,sixth week and the eighth week after the treatment,the concentration of KGF-1 in GCF was lower than inflammatory group before treatment and significant deference of KGF-1 concentration and statistical significance were found before treatment and in different stages after treatment (P <0.05). The

  6. 大鼠正畸牙根吸收早期龈沟液中DPP的变化规律%Dentin phosphoprotein in gingival crevicular fluid during early orthodontic root resorption in rats.

    Institute of Scientific and Technical Information of China (English)

    李文星; 刘月华

    2011-01-01

    目的:构建大鼠正畸牙根吸收模型,检测龈沟液中牙本质磷蛋白(DPP)的浓度变化规律.方法:雌性12周龄SD大鼠46只,其中16只取上颌第一磨牙及牙周组织,通过HE和TRAP染色观察不同时间点根吸收程度;另30只随机分为2组,持续加力28 d(CF组)和加力14 d后停止加力(IF组),收集龈沟液并通过ELISA检测DPP浓度.结果:持续加力14 d,DPP浓度逐渐升高,CF组与IF组间无显著差异(P>0.05);第14 d到28 d,CF组DPP浓度逐渐降低,而IF组DPP浓度在第21 d仍升高,组间有显著差异(P<0.01).根吸收相对面积和DPP浓度显著相关(P<0.05).结论:DPP作为牙的特异性蛋白,与正畸牙根吸收的修复相关,可能是早期诊断正畸牙根吸收的指标.%Objective: This study aimed to establish the animal model of early orthodontic root resorption in rats, and determine whether dentin phosphoprotein (DPP) in gingival crevicular fluid (GFC) could serve as a prognostic factor for orthodontic root resorption. Method:46 12-week- old female SD rats were selected. The maxillary left first molar was drawn medially by applying an excess force. 16 rats were killed and the sections were treated with HE and TRAP histochemistry in the aim of observing the root resorption. The other 30 rats were randomly divided into continuous force group (CF) and the force suspended group (IF) and GFC was collected. The concentrasion of DPP were assayed by using the biochemistry techniques of ELISA. Result:From Od to 14d afterburner,concentration of DPP gradually increased, there were no significant differences between the two groups (P >O.05);from 14 d to 28 d, the concentration of DPP decreased in CF group,while it remained elevated on the 21th day,then reduced a little in IF group. The data between the two groups was statistically significant difference (P <0.01). The relative area of root resorption was correlated to the concentration of DPP,and the Pearson correlation coefficient was 0

  7. Oxidized low-density lipoprotein-induced periodontal inflammation is associated with the up-regulation of cyclooxygenase-2 and microsomal prostaglandin synthase 1 in human gingival epithelial cells

    Energy Technology Data Exchange (ETDEWEB)

    Nagahama, Yu [Department of Periodontology, School of Dentistry, Showa University Dental Hospital, Tokyo (Japan); Department of Biological Chemistry, Showa University School of Pharmacy, Tokyo (Japan); Obama, Takashi [Department of Biological Chemistry, Showa University School of Pharmacy, Tokyo (Japan); Usui, Michihiko [Department of Periodontology, School of Dentistry, Showa University Dental Hospital, Tokyo (Japan); Kanazawa, Yukari [Department of Biological Chemistry, Showa University School of Pharmacy, Tokyo (Japan); Iwamoto, Sanju [Department of Biochemistry, Showa University School of Medicine, Tokyo (Japan); Suzuki, Kazushige [Department of Periodontology, School of Dentistry, Showa University Dental Hospital, Tokyo (Japan); Miyazaki, Akira [Department of Biochemistry, Showa University School of Medicine, Tokyo (Japan); Yamaguchi, Tomohiro [Department of Biological Chemistry, Showa University School of Pharmacy, Tokyo (Japan); Yamamoto, Matsuo [Department of Periodontology, School of Dentistry, Showa University Dental Hospital, Tokyo (Japan); Itabe, Hiroyuki, E-mail: h-itabe@pharm.showa-u.ac.jp [Department of Biological Chemistry, Showa University School of Pharmacy, Tokyo (Japan)

    2011-10-07

    Highlights: {yields} OxLDL-induced responses in human gingival epithelial cells were studied. {yields} OxLDL enhanced the production of IL-8, IL-1{beta} and PGE{sub 2} in Ca9-22 cells. {yields} An NF-{kappa}B inhibitor suppressed the expression of COX-2 and mPGES1 induced by oxLDL. {yields} Unlike the case in macrophages, oxLDL did not increase the CD36 level. -- Abstract: Periodontitis is characterized by chronic gingival tissue inflammation, and inflammatory mediators such as IL-8 and prostaglandin E{sub 2} (PGE{sub 2}) are associated with disease progression. Previously we showed that oxidatively modified low-density lipoprotein (oxLDL) was present in gingival crevicular fluid. In this study, the role of oxLDL in the gingival epithelial cell inflammatory response was further investigated using Ca9-22 cells and primary human oral keratinocytes (HOK). Treatment of Ca9-22 cells and HOK with oxLDL induced an up-regulation of IL-8 and the PGE{sub 2}-producing enzymes, cyclooxygenase-2 and microsomal PGE{sub 2} synthase-1. These responses induced by oxLDL were significantly suppressed by a nuclear factor-kappa B (NF-{kappa}B) inhibitor. However, unlike the result in macrophages, oxLDL did not lead to an increase in CD36 expression in these two cells. These results suggest that oxLDL elicits gingival epithelial cell inflammatory responses through an activation of the NF-{kappa}B pathway. These data suggest a mechanistic link between periodontal disease and lipid metabolism-related disorders, including atherosclerosis.

  8. Effect of different porcelain fused to metal crowns on gingival crevicular fluid%不同种类金属烤瓷底冠与基牙龈沟液的变化

    Institute of Scientific and Technical Information of China (English)

    孙培音; 王亚锋; 法永红; 蔡兴伟

    2011-01-01

    背景:目前关于非贵金属烤瓷和贵金属烤瓷对牙龈、牙周组织健康影响的研究较多,纯钛与非贵金属、贵金属之间进行比较的研究较少.目的:观察不同种类烤瓷金属材料修复后基牙龈沟液中龈沟液分泌量以及肿瘤坏死因子α和白细胞介素6水平的变化.方法:选择后牙区(前磨牙和磨牙)患者60例,共80颗受试牙,按烤瓷金属内冠材料不同分为3组,钴铬合金组28颗、纯钛烤瓷组32颗、金铂合金烤瓷组30颗.在修复1,3个月后检测龈沟液的分泌量以及肿瘤坏死因子α和白细胞介素6水平.结果与结论:修复后1,3个月钴铬合金组龈沟液分泌量以及肿瘤坏死因子α和白细胞介素6水平明显高于修复前(P 0.05).修复后1,3个月金铂合金组的各项指标与修复前比较差异无显著性意义(P > 0.05).钴铬合金组龈沟液分泌量、肿瘤坏死因子α和白细胞介素6水平高于纯钛组、金铂合金组(P < 0.05).纯钛组龈沟液分泌量、肿瘤坏死因子α水平高于金铂合金组(P < 0.05).%BACKGROUND: The majority of current researches focus on the influence of non-precious metal ceramic porcelain and metal ceramic porcelain on the gingival and periodontal tissue health, but few study reports the effect of pure titanium versus non-precious metals and precious metals.OBJECTIVE: To investigate the changes of gingival crevicular fluid (GCF) secretion, and the level of tumor necrosis factor-α (TNF-α) and interleu kin-6 (IL-6) in GCF during different materials of porcelain fused-to-metal crowns restoration.METHODS: Sixty patients including 80 premolar and molar teeth were involved as test teeth and divided into three groups according to the materials of porcelain fused-to-metal crowns, Co-Cr alloy group (n=28), pu re Ti alloy group (n=32) and gold-platinum alloy group (n=30). At 1 and 3 months after the restorations of crowns, the secretion amounts of GCF, the levels of TNF-α and IL-6 in GCF were

  9. Cleavage of host cytokeratin-6 by lysine-specific gingipain induces gingival inflammation in periodontitis patients.

    Directory of Open Access Journals (Sweden)

    Salunya Tancharoen

    Full Text Available Lysine-specific gingipain (Kgp is a virulence factor secreted from Porphyromonas gingivalis (P. gingivalis, a major etiological bacterium of periodontal disease. Keratin intermediate filaments maintain the structural integrity of gingival epithelial cells, but are targeted by Kgp to produce a novel cytokeratin 6 fragment (K6F. We investigated the release of K6F and its induction of cytokine secretion.K6F present in the gingival crevicular fluid of periodontal disease patients and in gingipain-treated rat gingival epithelial cell culture supernatants was measured by matrix-assisted laser desorption/ionization time-of-flight mass spectrometer-based rapid quantitative peptide analysis using BLOTCHIP. K6F in gingival tissues was immunostained, and cytokeratin 6 protein was analyzed by immunofluorescence staining and flow cytometry. Activation of MAPK in gingival epithelial cells was evaluated by immunoblotting. ELISA was used to measure K6F and the cytokines release induced by K6F. Human gingival fibroblast migration was assessed using a Matrigel invasion chamber assay.We identified K6F, corresponding to the C-terminus region of human cytokeratin 6 (amino acids 359-378, in the gingival crevicular fluid of periodontal disease patients and in the supernatant from gingival epithelial cells cultured with Kgp. K6F antigen was distributed from the basal to the spinous epithelial layers in gingivae from periodontal disease patients. Cytokeratin 6 on gingival epithelial cells was degraded by Kgp, but not by Arg-gingipain, P. gingivalis lipopolysaccharide or Actinobacillus actinomycetemcomitans lipopolysaccharide. K6F, but not a scrambled K6F peptide, induced human gingival fibroblast migration and secretion of interleukin (IL-6, IL-8 and monocyte chemoattractant protein-1. These effects of K6F were mediated by activation of p38 MAPK and Jun N-terminal kinase, but not p42/44 MAPK or p-Akt.Kgp degrades gingival epithelial cell cytokeratin 6 to K6F that, on

  10. Regulation of NAMPT in Human Gingival Fibroblasts and Biopsies

    Directory of Open Access Journals (Sweden)

    Anna Damanaki

    2014-01-01

    Full Text Available Adipokines, such as nicotinamide phosphoribosyltransferase (NAMPT, are molecules, which are produced in adipose tissue. Recent studies suggest that NAMPT might also be produced in the tooth-supporting tissues, that is, periodontium, which also includes the gingiva. The aim of this study was to examine if and under what conditions NAMPT is produced in gingival fibroblasts and biopsies from healthy and inflamed gingiva. Gingival fibroblasts produced constitutively NAMPT, and this synthesis was significantly increased by interleukin-1β and the oral bacteria P. gingivalis and F. nucleatum. Inhibition of the MEK1/2 and NFκB pathways abrogated the stimulatory effects of F. nucleatum on NAMPT. Furthermore, the expression and protein levels of NAMPT were significantly enhanced in gingival biopsies from patients with periodontitis, a chronic inflammatory infectious disease of the periodontium, as compared to gingiva from periodontally healthy individuals. In summary, the present study provides original evidence that gingival fibroblasts produce NAMPT and that this synthesis is increased under inflammatory and infectious conditions. Local synthesis of NAMPT in the inflamed gingiva may contribute to the enhanced gingival and serum levels of NAMPT, as observed in periodontitis patients. Moreover, local production of NAMPT by gingival fibroblasts may represent a possible mechanism whereby periodontitis may impact on systemic diseases.

  11. Leptin and Pro-Inflammatory Stimuli Synergistically Upregulate MMP-1 and MMP-3 Secretion in Human Gingival Fibroblasts.

    Directory of Open Access Journals (Sweden)

    Rachel C Williams

    Full Text Available Gingival fibroblast-mediated extracellular matrix remodelling is implicated in the pathogenesis of periodontitis, yet the stimuli that regulate this response are not fully understood. The immunoregulatory adipokine leptin is detectable in the gingiva, human gingival fibroblasts express functional leptin receptor mRNA and leptin is known to regulate extracellular matrix remodelling responses in cardiac fibroblasts. We therefore hypothesised that leptin would enhance matrix metalloproteinase secretion in human gingival fibroblasts.We used in vitro cell culture to investigate leptin signalling and the effect of leptin on mRNA and protein expression in human gingival fibroblasts. We confirmed human gingival fibroblasts expressed cell surface leptin receptor, found leptin increased matrix metalloproteinase-1, -3, -8 and -14 expression in human gingival fibroblasts compared to unstimulated cells, and observed that leptin stimulation activated MAPK, STAT1/3 and Akt signalling in human gingival fibroblasts. Furthermore, leptin synergised with IL-1 or the TLR2 agonist pam2CSK4 to markedly enhance matrix metalloproteinase-1 and -3 production by human gingival fibroblasts. Signalling pathway inhibition demonstrated ERK was required for leptin-stimulated matrix metalloproteinase-1 expression in human gingival fibroblasts; whilst ERK, JNK, p38 and STAT3 were required for leptin+IL-1- and leptin+pam2CSK4-induced matrix metalloproteinase-1 expression. A genome-wide expression array and gene ontology analysis confirmed genes differentially expressed in leptin+IL-1-stimulated human gingival fibroblasts (compared to unstimulated cells were enriched for extracellular matrix organisation and disassembly, and revealed that matrix metalloproteinase-8 and -12 were also synergistically upregulated by leptin+IL-1 in human gingival fibroblasts.We conclude that leptin selectively enhances the expression and secretion of certain matrix metalloproteinases in human gingival

  12. Effects of Calendula officinalis on human gingival fibroblasts.

    Science.gov (United States)

    Saini, Pragtipal; Al-Shibani, Nouf; Sun, Jun; Zhang, Weiping; Song, Fengyu; Gregson, Karen S; Windsor, L Jack

    2012-04-01

    Calendula officinalis is commonly called the marigold. It is a staple topical remedy in homeopathic medicine. It is rich in quercetin, carotenoids, lutein, lycopene, rutin, ubiquinone, xanthophylls, and other anti-oxidants. It has anti-inflammatory properties. Quercetin, one of the active components in Calendula, has been shown to inhibit recombinant human matrix metalloproteinase (MMP) activity and decrease the expression of tumor necrosis factor-α, interleukin-1β (IL), IL-6 and IL-8 in phorbol 12-myristate 13-acetate and calcium ionophore-stimulated human mast cells. To examine the effects of Calendula on human gingival fibroblast (HGF) mediated collagen degradation and MMP activity. Lactate dehydrogenate assays were performed to determine the non-toxic concentrations of Calendula, doxycycline and quercetin. Cell-mediated collagen degradation assays were performed to examine the inhibitory effect on cell-mediated collagen degradation. Gelatin zymography was performed to examine their effects on MMP-2 activity. The experiments were repeated three times and ANOVA used for statistical analyses. Calendula at 2-3% completely inhibited the MMP-2 activity in the zymograms. Doxycycline inhibited HGF-mediated collagen degradation at 0.005, 0.01, 0.02 and 0.05%, and MMP-2 activity completely at 0.05%. Quercetin inhibited HGF-mediated collagen degradation at 0.005, 0.01 and 0.02%, and MMP-2 activity in a dose-dependent manner. Calendula inhibited HGF-mediated collagen degradation and MMP-2 activity more than the same correlated concentration of pure quercetin. Calendula inhibits HGF-mediated collagen degradation and MMP-2 activity more than the corresponding concentration of quercetin. This may be attributed to additional components in Calendula other than quercetin. Published by Elsevier Ltd.

  13. HIV+牙周炎患者龈沟液IL-6的检测分析%Analysis of IL-6 in gingival crevicular fluid of HIV-positive patients with periodontitis

    Institute of Scientific and Technical Information of China (English)

    侯雯; 贾洪诚; 王璇; 华文浩; 付茜; 李晓光

    2014-01-01

    目的:初步探讨HIV+牙周炎患者龈沟液中IL-6和CD4+T淋巴细胞计数与牙周临床指标的相关性,为临床提供参考。方法记录20例HIV+牙周炎患者共120颗指数牙的牙周临床指标:菌斑指数(PLI)、出血指数(BI)、附着水平(AL)和牙周探诊深度(PD),用放射免疫法检测龈沟液中IL-6。计数受试者血液的CD4+T淋巴细胞,并按其分组:A组(CD4计数>500 cells/mm3,2例12颗牙),B组(CD4计数200~500 cells/mm3,13例78颗牙),C组(CD4计数<200 cells/mm3,5例30颗牙)。组间牙周临床指标和IL-6比较采用Mann-Whitney秩和检验,CD4+T淋巴细胞计数和牙周临床指标的关系采用偏相关分析,IL-6和牙周临床指标的关系采用Spearman相关分析。结果 B组的BI值、GCF值、IL-6浓度分别为3.00(2.00)、4.80(2.20)ml、10.36(5.54)pg/μl。三组间的PD、AL差异均无统计学意义。B组和C组的CD4计数和BI有相关性(P<0.05)。IL-6含量与各项牙周临床指标呈正相关(P<0.05);IL-6浓度与各项牙周临床指标呈负相关(P<0.05)。结论 HIV+牙周炎患者的牙周炎症程度与CD4+T淋巴细胞计数有关,其龈沟液中IL-6水平与牙周临床指标有关。%Objective To investigate the relationship between IL-6, CD4+T lymphocyte counts and periodontal status of HIV+patients with periodontitis. Methods The gingival crevicular fluid were collected from 120 teeth of 20 patients. The levels of IL-6 were determined by RIA assays. Clinical measurements were recorded, including plaque index(PLI), bleeding index(BI), attachment level(AL) and probing depth(PD). The plasmatic CD4+T lymphocytes were counted. All the individuals were divided into three groups:A group(CD4>500 cells/mm3), B group(200 cells/mm3≤CD4≤500 cells/mm3) and C group (CD4<200 cells/mm3). Mann-Whitney were used to compare groups. Partial correlations and Spearman correlations were applied to

  14. Binding, uptake, and release of nicotine by human gingival fibroblasts

    Energy Technology Data Exchange (ETDEWEB)

    Hanes, P.J.; Schuster, G.S.; Lubas, S. (Medical College of Georgia, Augusta (USA))

    1991-02-01

    Previous studies of the effects of nicotine on fibroblasts have reported an altered morphology and attachment of fibroblasts to substrates and disturbances in protein synthesis and secretion. This altered functional and attachment response may be associated with changes in the cell membrane resulting from binding of the nicotine, or to disturbances in cell metabolism as a result of high intracellular levels of nicotine. The purpose of the present study, therefore, was to (1) determine whether gingival fibroblasts bound nicotine and if any binding observed was specific or non-specific in nature; (2) determine whether gingival fibroblasts internalized nicotine, and if so, at what rate; (3) determine whether gingival fibroblasts also released nicotine back into the extracellular environment; and (4) if gingival fibroblasts release nicotine intact or as a metabolite. Cultures of gingival fibroblasts were prepared from gingival connective tissue biopsies. Binding was evaluated at 4{degree}C using a mixture of {sup 3}H-nicotine and unlabeled nicotine. Specific binding was calculated as the difference between {sup 3}H-nicotine bound in the presence and absence of unlabeled nicotine. The cells bound 1.44 (+/- 0.42) pmols/10(6) cells in the presence of unlabeled nicotine and 1.66 (+/- 0.55) pmols/10(6) cells in the absence of unlabeled nicotine. The difference was not significant. Uptake of nicotine was measured at 37{degree}C after treating cells with {sup 3}H-nicotine for time periods up to 4 hours. Uptake in pmols/10(6) cells was 4.90 (+/- 0.34) at 15 minutes, 8.30 (+/- 0.75) at 30 minutes, 12.28 (+/- 2.62) at 1 hour and 26.31 (+/- 1.15) at 4 hours.

  15. Expression of myeloperoxidase, soluble intercellular adhesion molecule-1 and pentraxin-3 in the gingival crevicular fluid during maxillary canine distal movement%上颌尖牙远中移动过程中龈沟液内中性粒细胞浸润炎症相关因子的表达

    Institute of Scientific and Technical Information of China (English)

    许桓溪; 邢洪波; 苗芳; 李宁; 邱静怡; 李娟

    2016-01-01

    BACKGROUND:To dynamicaly monitor the varying levels of inflammatory factors in the gingival crevicular fluid is helpful to assess the early effect of orthodontic tooth movement. Myeloperoxidase, soluble intercelular adhesion molecule-1, pentraxin 3 are proven to be closely related to inflammation, but it is unclear about the levels of these three kinds of inflammatory factors as wel as association of these three kinds of inflammatory factors with orthodontic tooth. OBJECTIVE:To detect the expression levels of myeloperoxidase, soluble intercelular adhesion molecule-1 and pentraxin-3 in the gingival crevicular fluid during maxilary canine distal movement and to assess their correlation with periodontal disease, canine movement distance and orthodontic force. METHODS:Twenty-one orthodontic patients were enroled and assigned into 150 g (n=12) or 100 g (n=9) groups according to orthodontic force. The gingival crevicular fluid samples of orthodontic patients were colected before and at 4, 12, 24 hours, 7, 14 days after maxilary canine distal movement. Levels of myeloperoxidase, soluble intercelular adhesion molecule-1 and pentraxin-3 in the gingival crevicular fluid were measured and analyzed using ELISA assay. RESULTS AND CONCLUSION: During the distal movement of maxilary canine, under orthodontic force, the level of myeloperoxidase was peaked at 4 hours and then decreased, while the expression level of soluble intercelular adhesion molecule-1 was peaked at 12 hours, and then decreased. Both myeloperoxidase and soluble intercelular adhesion molecule-1 levels returned to normal at 7 days under orthodontic force. The expression level of pentraxin-3 was increased significantly under orthodontic force, peaked at 24 hours, and then decreased gradualy to the normal level at 7 days. In addition, the expression levels of myeloperoxidase, soluble intercelular adhesion molecule-1 and pentraxin-3 in the gingival crevicular fluid were significantly higher under 150 g force than

  16. Cytokine levels in crevicular fluid are less responsive to orthodontic force in adults than in juveniles

    NARCIS (Netherlands)

    Ren, Yijin; Maltha, Jaap C; Van't Hof, Martin A; Von Den Hoff, Johannes W; Kuijpers-Jagtman, Anne Marie; Zhang, Ding

    2002-01-01

    OBJECTIVES: Bone remodelling during orthodontic tooth movement is related to the expression of mediators in gingival crevicular fluid (GCF). No information is available concerning the effect of age on the levels of these mediators in GCF. The purpose of this study was to quantify three mediators (pr

  17. Prevalence of herpesviruses in gingivitis and chronic periodontitis: relationship to clinical parameters and effect of treatment

    Directory of Open Access Journals (Sweden)

    Rucha Shah

    2016-01-01

    Full Text Available Background: Assess the prevalence of herpesviruses in healthy subjects, gingivitis, and chronic periodontitis patients, to assess the relationship between the prevalence of herpesviruses and periodontal clinical parameters, and to evaluate the effect of phase-I therapy on the level of viral detection. Materials and Methods: Hundred patients consisting of 20 healthy subjects, 40 gingivitis, and 40 chronic periodontitis were included in the study. Clinical parameters recorded included plaque index, gingival index, sulcus bleeding index, probing depth, and clinical attachment level. The gingivitis and chronic periodontitis patients received phase-I periodontal therapy including oral hygiene instructions, full mouth scaling for gingivitis patients and scaling and root planing for chronic periodontitis patients. Gingival crevicular fluid (GCF was collected, and the presence of herpes simplex virus-1 (HSV-1, HSV-2, cytomegalovirus, and Epstein–Barr virus (EBV was analyzed using polymerase chain reaction (PCR. Recording of periodontal parameters as well as GCF collection was performed at baseline and 6 weeks postphase-I therapy. Results: At baseline, the levels of HSV-1 and EBV detection were lower in healthy controls as compared to gingivitis (P < 0.05 and chronic periodontitis cases (P < 0.001. Phase-I therapy led to reduction in the amount of HSV-1 and EBV in gingivitis patients (P < 0.05 and for HSV-1, human cytomegalovirus and EBV in chronic periodontitis patients (P < 0.05 in comparison to baseline. The prevalence of EBV in chronic periodontitis patients was positively associated with increased gingival index, probing depth and loss of clinical attachment (P < 0.05. Conclusions: Higher prevalence of HSV-1 and EBV viruses in GCF of gingivitis and chronic periodontitis suggests a strong association between these viruses and periodontal diseases and periodontal therapy can lead to a reduction in herpesviruses at infected sites.

  18. Effect of IPS e.max Press Ceramic Crown on the Level of MMP-2 in Gingival Crevicular Fluid%IPS e.max Press铸瓷全冠对龈沟液中MMP-2水平的影响

    Institute of Scientific and Technical Information of China (English)

    董蕾; 孙振学; 朱海燕

    2015-01-01

    Objective To observe the ef ects of IPS e.max press ceramic crown on the periodontal tissue of the restored teeth. Methods 79 clinical patients (107 anterior teeth)were selected to be placed by IPS e.max press ceramic crown ,and before and 6 months after rehab,the weight of gingival crevicular fluid (GCF)was mensurated and the level of MMP-2 in GCF was checked by way of enzyme-linked immuno sorbent assay,the result was analyzed. Results The weight of GCF,the level of MMP-2 in GCF after rehab had no significantly statistical dif erence with those before rehab(﹥0.05). Conclusion IPS e.max press ceramic crown has no significantly negative influence on gingival health of the restored teeth. And it is a kind of restoration with favorable biocompatibility.%目的:探讨IPS e.max Pres 铸瓷全冠修复对牙周组织的影响。方法选择IPS e.max Pres 铸瓷全冠修复患者79例107颗患牙,在修复前和修复后6个月,测定龈沟液(GCF)量,并运用采用酶联免疫吸附试验(ELISA)对龈沟液中基质金属蛋白酶-2(MMP-2)水平进行分析。结果修复后GCF量、MMP-2水平与修复前比较差异无统计学意义(﹥0.05)。结论 IPS e.max Pres 铸瓷全冠对牙周组织无明显影响,是一种生物相容性良好的修复方式。

  19. Effect of Lactobacillus reuteri on Cell Viability and PGE2 Production in Human Gingival Fibroblasts

    DEFF Research Database (Denmark)

    Castiblanco, Gina A.; Yucel-Lindberg, Tulay; Roos, Stefan

    2017-01-01

    Emerging evidence suggests that probiotic therapy can play a role in the prevention and management of oral inflammatory diseases through immunomodulation and down-regulation of the inflammatory cascade. The aim of this in vitro study was to investigate the viability of human gingival fibroblasts...... immune assay kits. Our findings showed that none of the L. reuteri supernatants were cytotoxic or affected the viability of HGF. The most concentrated bacterial supernatant stimulated the production of PGE2 by the gingival cells in a significant way in the presence of IL-1β (p ... that bacterial products secreted from L. reuteri might play a role in the resolution of inflammation in HGF. Thus, our findings justify further investigations on the influence of probiotic bacteria on gingival inflammatory reactions....

  20. 慢性阻塞性肺疾病患者牙周病及外周血、龈沟液炎性标志物观察%Oral Hygiene Status and Observation of Inflammatory Factors in Peripheral Blood and Gingival Crevicular Fluid of Chronic Obstructive Pulmonary Disease

    Institute of Scientific and Technical Information of China (English)

    李方治; 侯春阳; 王笑歌

    2014-01-01

    Objective To compare the oral hygiene status and the observation of inflammatory factors in the peripher-al blood and gingival crevicular fluid (GCF) of chronic obstructive pulmonary disease (COPD). Methods 51 COPD pa-tients and 57 healthy controls were selected from the outpatient department and wards of Department Respiratory Diseases of the Fourth Affiliated Hospital of China Medical University during January 2012 to October 2013. Periodontal examination and measurements of inflammatory factors, including IL-1β, IL-6, TNF-αand hs-CRP, in the peripheral blood and GCF were de-tected in the two groups. Results The periodontitis prevalence of COPD group (56. 86%, n=29) was significantly higher than that of control group (21. 05%, n=12) (χ2 =14. 66, P<0. 01). The clinical attachment loss, periodontal probing depth, gingival index, sulcular bleeding index, plaque index, tooth mobility, dental fillings index and simplified oral hygiene index of the COPD group were significantly lower than those of the control group (P<0. 05). IL-1β, IL-6, TNF-α and hs-CRP in the peripheral blood of COPD group were notably higher than those of the control group;in addition, the GCF, IL-1β, IL-6 and TNF-α in the COPD group were still higher than those of control group (P<0. 05). Conclusion The oral hygiene status is poor in COPD patients, which increases the prevalence of periodontal disease. Therefore the expression levels of in-flammatory factors in peripheral blood and GCF are significantly increased.%目的:观察慢性阻塞性肺疾病( chronic obstructive pulmonary disease, COPD)患者牙周病(牙周指标)及外周血、龈沟液( gingival crevicular fluid, GCF)炎性标志物情况。方法选取2012年1月—2013年10月在中国医科大学附属第四医院呼吸内科门诊和(或)住院治疗的COPD患者51例作为COPD组及同期健康体检的57例作为对照组。两组均行牙周检查及外周血、GCF 白细胞介素( IL )-1β、IL-6

  1. The influence of mesially impacted third molar on MMP8 and TIMP1 level in gingival crevicular fluid of adjacent second molar%第三磨牙近中阻生对邻近第二磨牙龈沟液中MMP8和TIMP1的影响

    Institute of Scientific and Technical Information of China (English)

    于书娟; 程磊; 柳玉晓; 单文玲; 黄小艳; 何玉宏; 朱国雄

    2012-01-01

    Objective:To study the influence of mesially impacted third molar on MMP8 and TIMP1 expression in gingival crevicular fluid ( GCF) of adjacent second molar. Methods: 64 soldiers were divided into four groups ( n = 16). In group A, the soldiers had second molars with normal- looking gingival flap overlying a mesially impacted mandibular third molar without history of pericoronitis. In group B, the soldiers had the same second and third mandibular molars as in group A but experienced pericoronitis of the third molar within six months. In group C the soldiers showed signs and symptoms of pericoronitis of the mandibular third molar. In group D the soldiers did not have the third molar and the mandibular second molars served as controls. GCF of second molars was collected, MMP8 and TIMP1 were measured. Sulcus bleeding index (SBI) , probing depth (PD) and plaque index (PI) of the related second molars were examined. Results: MMP-8 and TIMP1 concentrations in the gingival crevicular fluid in the four groups were different(C > B > A > D. A vs B, P > 0. 05 ; A vs C or D, B vs C or D and C vs D, P < 0. 05 ) . In clinical indexes, PI of group C was higher than that of group D (P < 0. 05) , and PD in group A, B and C was deeper than in group D (P < 0.05). Conclusion: The mesially impacted third molars may stimulate the MMP8,TIMP1, MMP8/TIMP1 expression and increase PD of the adjacent second molar, and they might be potential risk factors of periodontal inflammation of the second molar. Pericoronitis of the third molar might increased the effects.%目的:探讨第三磨牙近中阻生对邻近磨牙龈沟液中MMP8和TIMP1的影响.方法:选择64例口腔科就诊的战士,分为4组,A组下颌第二磨牙伴第三磨牙近中阻生,第三磨牙无冠周炎病史,龈瓣颜色正常;B组下颌第二磨牙伴第三磨牙近中阻生,6个月内第三磨牙有冠周炎病史,龈瓣颜色正常;C组下颌第二磨牙伴第三磨牙近中阻生,有冠周炎;D组无下颌第

  2. Gingival Enlargement

    Science.gov (United States)

    ... 2) medication-induced gingival enlargement, 3) hereditary gingival fibromatosis, and 4) systemic causes of gingival enlargement. Inflammatory ... hygiene measures will reduce the severity. Hereditary Gingival Fibromatosis This is a rare hereditary condition that usually ...

  3. Assessment of human gingival fibroblast interaction with dental implant abutment materials.

    Science.gov (United States)

    Rutkunas, Vygandas; Bukelskiene, Virginija; Sabaliauskas, Vaidotas; Balciunas, Evaldas; Malinauskas, Mangirdas; Baltriukiene, Daiva

    2015-04-01

    The biocompatibility of dental implant abutment materials depends on numerous factors including the nature of the material, its chemical composition, roughness, texture, hydrophilicity and surface charge. The aim of the present study was to compare the viability and adhesion strength of human gingival fibroblasts (HGFs) grown on several dental materials used in implant prosthodontics. Surfaces of the tested materials were assessed using an optical imaging profiler. For material toxicity and cellular adhesion evaluation, primary human gingival fibroblast cells were used. To evaluate the strength of cellular adhesion, gingival fibroblasts were cultured on the tested materials and subjected to lateral shear forces by applying 300 and 500 rpm shaking intensities. Focal adhesion kinase (FAK) expression and phosphorylation in cells grown on the specimens were registered by cell-based ELISA. There was a tendency of fibroblast adhesion strength to decrease in the following order: sandblasted titanium, polished titanium, sandblasted zirconium oxide, polished zirconium oxide, gold-alloy, chrome-cobalt alloy. Higher levels of total as well as phospho-FAK protein were registered in HGFs grown on roughened titanium. Material type and surface processing technique have an impact on gingival fibroblast interaction with dental implant abutment materials.

  4. The effectiveness of potent dental adhesives on the viability of LPS challenged human gingival fibroblasts.

    Science.gov (United States)

    Garner, Angelia D; Tucci, Michelle A; Benghuzzi, Hamed A

    2014-01-01

    Dental adhesives are necessary for the retention of specific dental restorations utilized to repair the anatomy of the tooth after dental decay is removed. Adhesives come into contact with healthy and diseased periodontal tissues. Porphyromonas gingivalis is a gram negative bacterial pathogen, and lipopolysaccharide (LPS-PG) is an endotoxin found in gingival connective tissues of patients who suffer from periodontal disease. The presence of the endotoxin causes inflammation. This study aims to evaluate the effectiveness of potent dental adhesives when human gingival fibroblasts are challenged with LPS-PG. The fibroblasts were exposed to the dental adhesives polymethly methacrylate (PMMA), OptiBond®, and Prime & Bond® which were purchased from Patterson Dental, a national dental materials supplier. The human gingival fibroblasts (HGF-1, ATCC® CRL-2014™) were purchased from American Type Culture Collection (ATCC). The porphyromonas gingival lipopolysaccharide (LPS-PG) was purchased from Fisher Scientific (Pittsburg, PA). No significant differences in metabolic behavior was detected among the groups (padhesives and LPS-PG at 48 hour intervals (p<0.003). No significant changes were noted in cellular morphology at any phases, and all cells demonstrated typical fibroblast spindle shape.

  5. The relationship between two kinds of human herpesviruses and experimental gingivitis%两种人类疱疹病毒与实验性龈炎的关系

    Institute of Scientific and Technical Information of China (English)

    赵亦兵; 孟焕新

    2012-01-01

    Objective To investigate human cytomegalovirus (HCMV) and Epstein-Barr virus-type 1 (EBV-1) in GCF and saliva during experimental gingivitis in Chinese young subjects and to evaluate the effect of the virus in the initial stage of gingival inflammation.Methods GCF of 14 and 45 and saliva without stimulating in 11 Chinese young males with healthy gingiva were collected at baseline (day 0),day 7,14 and 21 after stopping oral hygiene and day7 after reestablishing oral hygiene(day 28).DNA of HCMV and EBV-1 were detected by nested-polymerase chain reaction (n-PCR) at the times mentioned above.Results HCMV was detected in GCF of 4 subjects at baseline,4 subjects at day 7,3 subjects at day 14 and 2 subjects at day 21 while the subjects were different.At day 28 HCMV could not be detected.EBV-1 was not detectable in GCF during the experimental gingivitis.HCMV was detected in saliva in 4 subjects and EBV-1 was in 3 subjects.And there is no relationship between the detection of the herpesviruses and the clinical parameters as well.Conclusion We suggest that HCMV and EBV-1 are not the important factors during the initial stage of gingival inflammation.%目的 检测人类巨细胞病毒(human cytomegalovirus,HCMV)和Epstein-Barr病毒-1型(Epstein-Barr virus-type1,EBV-1))在实验性龈炎过程中龈沟液(gingival crevicular fluid,GCF)和唾液中的检出变化,探讨病毒在牙龈炎症发生发展过程中的作用.方法 选取11名牙龈健康,无全身疾病的年轻男性受试者,在停止口腔卫生措施后21天内(第0、7、14、21天)和恢复口腔卫生措施一周后(第28天)收集14,45两颗牙的GCF以及受试者的唾液,使用巢式PCR技术检测HCMV和EBV-1在不同时间点的检出情况.结果 GCF中HCMV在基线、7、14 d和21 d时分别有4个、4个、3个和2个受试者检出,但检出的受试者在该过程中不一致,第28天时无受试者检出;EBV-1在GCF中均未检出.在唾液中,实验过程中不同时间点共仅有4名和3

  6. Alteration of metabolomic profiles by titanium dioxide nanoparticles in human gingivitis model.

    Science.gov (United States)

    Garcia-Contreras, Rene; Sugimoto, Masahiro; Umemura, Naoki; Kaneko, Miku; Hatakeyama, Yoko; Soga, Tomoyoshi; Tomita, Masaru; Scougall-Vilchis, Rogelio J; Contreras-Bulnes, Rosalia; Nakajima, Hiroshi; Sakagami, Hiroshi

    2015-07-01

    Although nanoparticles (NPs) has afforded considerable benefits in various fields of sciences, several reports have shown their harmful effects, suggesting the necessity of adequate risk assessment. To clarify the mechanism of titanium dioxide nanoparticles (TiO2 NPs)-enhanced gingival inflammation, we conducted the full-scale metabolomic analyses of human gingival fibroblast cells treated with IL-1β alone or in combination with TiO2 NPs. Observation with transmission electron microscope demonstrated the incorporation of TiO2 NPs into vacuoles of the cells. TiO2 NPs significantly enhanced the IL-1β-induced prostaglandin E2 production and COX-1 and COX-2 protein expression. IL-1β reduced the intracellular concentrations of overall primary metabolites especially those of amino acid, urea cycle, polyamine, S-adenosylmethione and glutathione synthetic pathways. The addition of TiO2 NPs further augmented these IL-1β-induced metabolic changes, recommending careful use of dental materials containing TiO2 NPs towards patients with gingivitis or periodontitis. The impact of the present study is to identify the molecular targets of TiO2 NPs for the future establishment of new metabolic markers and therapeutic strategy of gingival inflammation.

  7. Effect of Cyclosporin A and Angiotensin II on cytosolic calcium levels in primary human gingival fibroblasts

    Directory of Open Access Journals (Sweden)

    Ajitkumar Supraja

    2016-01-01

    Full Text Available Background: To evaluate the effect of Cyclosporin A (CsA and angiotensin II (Ang II on cytosolic calcium levels in cultured human gingival fibroblasts (HGFs. Materials and Methods: Healthy gingival samples from six volunteers were obtained, and primary HGFs were cultured. Cell viability and proliferation assay were performed to identify the ideal concentrations of CsA and Ang II. Cytosolic calcium levels in cultured gingival fibroblasts treated with CsA and Ang II were studied using colorimetric assay, confocal and fluorescence imaging. Statistical analyses were done using SPSS software and GraphPad Prism. Results: Higher levels of cytosolic levels were evident in cells treated with CsA and Ang II when compared to control group and was statistically significant (P < 0.05 in both colorimetric assay and confocal imaging. Fluorescent images of the cultured HGFs revealed the same. Conclusion: Thus calcium being a key player in major cellular functions, plays a major role in the pathogenesis of drug-induced gingival overgrowth.

  8. Gingival Recessions and Biomechanics

    DEFF Research Database (Denmark)

    Laursen, Morten Godtfredsen

    Gingival recessions and biomechanics “Tissue is the issue, but bone sets the tone.“ A tooth outside the cortical plate can result in loss of bone and development of a gingival recession. The presentation aims to show biomechanical considerations in relation to movement of teeth with gingival...... recessions. Gingival recession is a problem often in the region of the lower incisors. A micro-CT study on human autopsy material, performed at the University of Aarhus, confirmed that the anterior mandibular alveolar envelope is indeed very thin. The prognosis of a gingival recession can be improved...

  9. Observation of detection rate changes of herpes virus at different time points of periodontal mechanical treatment in patients with chronic periodontitis gingival crevicular fluid%慢性牙周炎进行机械治疗的不同时限龈沟液疱疹病毒检出率的变化研究

    Institute of Scientific and Technical Information of China (English)

    钟守鹏; 曹晖

    2015-01-01

    目的 通过观察牙周机械治疗慢性牙周炎患者治疗前及治疗后不同时间点龈沟液疱疹病毒检出率的变化,分析慢性牙周炎与疱疹病毒的关系,并且为牙周炎患者的治疗及预后提供一个微生物学依据.方法 随机选取2012年3月-2014年3月来我院口腔科进行牙周机械治疗的慢性牙周炎患者16例,采用巢式聚合酶链反应分别检测患者治疗前、治疗后2个周、2个月、4个月时点龈沟液中的巨细胞病毒(HCMB)和EB病毒1型(EBV-1),并分析两种病毒检出率的变化情况.结果 慢性牙周炎患者HCMB、EBV-1治疗前检出率分别为43.8%、15%,治疗后2个周时HCMV、EBV-1的检出率降至最低,分别为8.8%、0;治疗后2个周、2个月、4个月时HCMV和EBV的检出率均明显低于治疗前(P<0.05),HCMV在治疗后2个月的检出率(15%)比2周时(8%)有所回升,差异无统计学意义(P>0.05).结论 疱疹病毒在慢性牙周炎患者的发病中起一定作用,并且在牙周机械治疗后2个月内临床指标最为敏感,可以在治疗2周后复查以指导临床治疗.%Objective To observe the detection rate changes of herpes virus at different time points of periodontal mechanical treatment in patients with chronic periodontitis gingival crevicular fluid and analyze the relationship between chronic periodontitis and herpes virus,which provide a means of microbiological basis for the treatment and prognosis of patients with periodontitis.Methods 16 patients with chronic periodontitis periodontal mechanical treatment of cases were randomly selected as research subjects between March 2012-March 2014 in the department of stomatology in our hospital.HCMB) and EB virus type 1 (EBV-1) were detected in gingival crevicular fluid of cytomegalovirus by nested polymerase chain reaction before and after treatment for 2 weeks,2 months,4 months time and analysis of the changes in the incidence of two kinds of virus.Results HCMB and EBV-1

  10. Levels of glycosaminoglycans in gingival crevicular fluid of patients with periodontal degree II furcation involvement before and after guided tissue regeneration.%Ⅱ°根分叉病变患者引导组织再生治疗术前术后龈沟液中糖氨多糖水平的变化

    Institute of Scientific and Technical Information of China (English)

    闫福华; 郑瑜谦; 等

    2002-01-01

    目的观察Ⅱ°根分叉病变患者引导组织再生治疗术(guided tissue regeneration, GTR)前后龈沟液(gingival crevicular fluid, GCF)中糖氨多糖(glycosaminoglycans, GAG) 水平变化的同时,探讨GCF中GAG能否作为判断GTR术后组织成熟的指标.方法对6例Ⅱ°根分叉病变的患牙采用GTR治疗,并于手术前,手术后1、2、3、4、5、6周收集GCF.用0.1%阿尔辛兰(Alcian blue)染色,分光光度法测定GCF中总的硫酸化GAG及硫酸软骨素(chondroitin sulfate, CS)的水平.结果 GTR术后1~2周,GCF 中CS 明显降低(P<0.05),然后逐渐升高,第6周恢复到基线水平.而GCF中总的硫酸化GAG则在术后1周明显升高(P<0.05),然后下降,到第6周升高并超过基线水平.结论 GCF中总的硫酸化GAG,尤其是CS 可用作监测牙周伤口愈合和组织再生的一个潜在指标,但是否可以用作GTR术后组织成熟的指标,还需加大样本并结合病理进行进一步的纵向观察.

  11. Efficacy of scaling and root planning with and without adjunct Nd:YAG laser therapy on clinical periodontal parameters and gingival crevicular fluid interleukin 1-beta and tumor necrosis factor-alpha levels among patients with periodontal disease: A prospective randomized split-mouth clinical study.

    Science.gov (United States)

    Abduljabbar, Tariq; Vohra, Fahim; Kellesarian, Sergio Varela; Javed, Fawad

    2017-04-01

    Limited evidence exists regarding the role of scaling and root planning (SRP) with adjunct neodymium yttrium aluminum garnet (Nd:YAG) laser therapy in reducing periodontal parameters (plaque index [PI], bleeding on probing [BOP] and probing pocket depth [PPD]) and levels of proinflammatory cytokines in the gingival crevicular fluid (GCF) among patients with periodontal disease (PD). The aim was to assess the effect of SRP with and without adjunct Nd:YAG laser therapy on clinical periodontal parameters and GCF interleukin 1-beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) levels among patients with PD. Demographic data was collected using a questionnaire. Mandibular right and left quadrants were randomly divided into test- (SRP+Nd:YAG laser) and control-sites (SRP alone). PI, BOP and PPD were assessed and GCF IL-1β and TNF-α levels were measured at baseline and at 3- and 6-month follow-up. Level of significance was set at Pnd 6-month follow-up, PI (Pnd PPD (Pnd PPD and GCF IL-1β and TNF-α levels were comparable at 3- and 6-month follow-up. At 6-month follow-up, IL-1β (Pnd TNF-α (Pnd 6-month follow-up. At 3- and 6-month follow-up, SRP+Nd:YAG therapy was more effective in reducing periodontal inflammatory parameters and GCF IL-1β and TNF-α levels compared with SRP alone. Copyright © 2017. Published by Elsevier B.V.

  12. 正畸牙根吸收与龈沟液中牙本质涎磷蛋白和牙本质涎蛋白相关性的实验研究%Relationship between orthodontics root resorption following experimental tooth movement and the level ofdentin sialoph-osphoprotein and dentin siaioprotein in gingival crevicular fluid

    Institute of Scientific and Technical Information of China (English)

    左志刚; 胡敏; 姜欢; 田莉

    2011-01-01

    Objective To investigate the relationship of expression of dentin sialoph-osphoprotein (DSPP) and dentin sialoprotein (DSP) in gingival crevicular fluid (GCF) with root resorption following experimental tooth movement in rats.Methods 36 Wistar rats were divided into 3 groups on average randomly: Control group, light force group and heavy force group.The experimental teeth were drawn-off mesially by the force of 0.392 N in light force group and 0.98 N in heavy force group, with both of the maxillary central incisors as the tooth of anchorage.At the 7th day, the gingival crevicular fluid of rats were collected; the histological slices were made, including the experimental tooth and periodontal tissue; the tissues was stained with hematoxylin-eosin (HE) staining and tartrate resistant acid phosphatase (TRAP) staining to observe the histological changes of the root resorption of rats.Then the expression of DSPP and DSP were assayed by using biochemistry techniques of Western blot.Results Histological observation: There was not root resorption in control group.Neither root resorption nor cementoclast was observed in light force group.And in heavy force group visible root resorption came out in pressure zone.Western blot results: There was expression of DSPP and no DSP in control group, and there was the expression of DSPP and DSP in both light force group and heavy force group.The result of statistical analysis showed that there were significant differences in the expression of DSPP and DSP among three groups.The highest one was heavy force group, followed by the light force group and control group with the least amount of proteins.Conclusion There is the expression of DSPP and DSP in gingival crevicular fluid following experimental tooth movement with root resorption.%目的 探讨大鼠龈沟液中牙本质涎磷蛋白(DSPP)和牙本质涎蛋白(DSP)的表达与实验性牙移动所致牙根吸收的关系.方法 36只健康Wistardd大鼠随机分成3组:对照组、

  13. Resinous perforation-repair materials inhibit the growth, attachment, and proliferation of human gingival fibroblasts.

    Science.gov (United States)

    Huang, Fu-Mei; Tai, Kuo-Wei; Chou, Ming-Yung; Chang, Yu-Chao

    2002-04-01

    The choice of repair material is one of the important factors in the prognosis of the endodontically treated tooth with a perforation defect. The cytotoxicity of perforation-repair materials must be investigated to ensure a safe biological response. The aim of this in vitro study was to evaluate the effect of resin-modified, glass-ionomer cement, compomer, and resin on human-gingival fibroblasts. Human gingival fibroblasts from crown lengthening surgery were cultured by using an explant technique with the consent of the patient. Cytotoxicity was judged by using an assay of tetrazolium bromide reduction. The results showed that resin-modified, glass-ionomer cement Fuji II LC, compomer Compoglass, and resin SpectrumTPH (TPH) were cytotoxic to primary human gingival fibroblast cultures by inhibiting cell growth and proliferation. TPH alone had an effect on cell attachment. It was found that TPH was the most cytotoxic repair material among those tested in all cultures. The toxicity decreased in the order of TPH>FLC>CG.

  14. Elevated Snail expression in human gingival fibroblasts by cyclosporine A as the possible pathogenesis for gingival overgrowth

    Directory of Open Access Journals (Sweden)

    Yi-Hung Lin

    2015-12-01

    Conclusion: CsA stimulated Snail expression and cell proliferation in HGFs, while silencing Snail could effectively reverse these phenomena. These results may provide new avenues for the design of novel antifibrotic therapies for CsA-induced gingival overgrowth through targeting Snail.

  15. Preferential attachment of human gingival fibroblasts to the resin ionomer Geristore.

    Science.gov (United States)

    Al-Sabek, Fuwad; Shostad, Sandra; Kirkwood, Keith L

    2005-03-01

    The resin ionomer Geristore has been used extensively for root perforation repairs. The purpose of this study was to evaluate oral in vitro biocompatibility of the resin ionomer Geristore compared to two other dental perforation repair materials, Ketac-Fil and Immediate Restorative Material (IRM). Growth and morphology of human gingival fibroblasts (HGFs) was determined using scanning electron microscopy (SEM) of HGFs cells grown on test materials as well as cytotoxicity assays using eluates from test materials. SEM analysis showed that HGFs attached and spread well over Geristore with relatively normal morphology. SEM showed that fibroblasts did not attach and spread well over Ketac-Fil or IRM as cells appeared much fewer with rounded and different morphology than fibroblasts grown on Geristore. Cytotoxicity assays indicated that HGFs proliferated in the presence of Geristore eluates and not in the presence of Ketac-Fil or IRM eluates. In vitro interpretation indicates that Geristore is less cytotoxic to gingival fibroblasts.

  16. Early supra- and subgingival plaque formation in experimental gingivitis in smokers and never-smokers.

    Science.gov (United States)

    Branco, Paula; Weidlich, Patricia; Oppermann, Rui Vicente; Rösing, Cassiano Kuchenbecker

    2015-01-01

    To evaluate supragingival and subgingival plaque formation on the dentogingival area in smokers and never smokers using the experimental gingivitis model and a plaque scoring system that considers the presence of an area free of plaque between plaque and the gingival sulcus called the plaque free zone (PFZ). Male volunteers, 9 current smokers and 10 never-smokers, refrained from oral hygiene procedures in the maxillary incisors and canines (test teeth) for 25 days. Under conditions of clinically healthy gingiva (phase 1) and gingival inflammation (phase 2), the supragingival plaque formation pattern was observed for 4 days in the dentogingival area. Gingival crevicular fluid was also measured. Plaque was dyed with fucsine and its presence was recorded by a calibrated examiner based on a 3-criteria scoring system: 0 - absence of stained plaque; 1 - presence of stained plaque and supragingival PFZ; 2 - presence of stained plaque and absence of PFZ, indicating that subgingival plaque formation has taken place. In both phases, smokers presented a significantly lower relative frequency of sites with subgingival plaque compared to never-smokers (P < 0.001). Mean gingival crevicular fluid was significantly higher in the presence of gingival inflammation for both groups (P = 0.001), whereas smokers demonstrated a significantly lower frequency of gingival bleeding than did non-smokers (23.6% vs 66.1%; P < 0.001). Smokers presented significantly lower percentages of sites with subgingival plaque in all experimental periods and presented less gingival inflammation as shown by GBI and gingival crevicular fluid quantification.

  17. Effect of three commercial mouth rinses on cultured human gingival fibroblast: An in vitro study

    Directory of Open Access Journals (Sweden)

    Flemingson

    2008-01-01

    Full Text Available Aim: To examine the effect of three commercial mouth rinses (Hexidine 0.2%, Listerine Cool Mint, Betadine 1% upon cultured human gingival fibroblast proliferation. Materials and Methods: Human gingival fibroblasts were cultured and incubated in Dulbecco′s Minimum Eagle′s Medium containing Chlorhexidine, Listerine, Povidone-Iodine at varying concentrations (1%, 2%, 5%, 10%, 20% and 100% of the given solution at 37°C for 1, 5 and 15 min. Control cells received an equal volume of Dulbecco′s Minimum Eagle′s Medium without adding mouth rinses, for similar duration of exposure at 37°C. Following incubation the media were removed, cells were washed twice with medium, supplemented with 10% Fetal Bovine Serum, and fibroblasts in the test and control group were allowed to recover in the same media for 24 h. Results: In all the three groups, the proliferation inhibition was dependent on the concentration of solublized mouth rinses in the cell culture but independent of the duration of exposure to all three mouth rinses. The results showed that all three solutions were toxic to cultured human gingival fibroblasts, Chlorhexidine being the most cytotoxic. It was seen that at dilute concentrations (1% and 2% of given solutions Listerine was more cytotoxic than Chlorhexidine and Povidone-Iodine. Conclusion: These results suggest that Chlorhexidine, Listerine and Povidone-Iodine are capable of inducing a dose-dependent reduction in cellular proliferation of fibroblasts. The results presented are interesting, but to know the clinical significance, further studies are needed.

  18. 冠心病伴牙周炎患者非刺激性唾液及龈沟液中血小板活化因子的检测及意义%Detection of platelet-activating factor in unstimulated mixed saliva and gingival crevicular fluid of periodontitis patients with coronary heart disease

    Institute of Scientific and Technical Information of China (English)

    冯伟; 吴婷; 孙钦峰; 杨丕山; 刘晓玲

    2010-01-01

    目的:检测冠心病伴中重度牙周炎、单纯冠心病、单纯中重度牙周炎及健康对照组的非刺激性全唾液(unstimulated mixed saliva)及龈沟液(gingival crevicular fluid,GCF)中血小板活化因子(platelet-activating factor,PAF)水平,探讨牙周炎与心血管疾病之间的关系.方法:选取经冠状动脉造影确诊为冠心病且伴有中重度牙周炎患者24例(C+P组),单纯冠心病患者36例(C组),单纯中重度牙周炎患者32例(P组)及健康人(H组)28例.对所有受检者进行口腔检查,记录其探诊出血指数、牙石指数、探诊深度、附着丧失等相关临床牙周指标,并收集非刺激性全唾液和龈沟液,采用ELISA检测其中PAF水平.各组间PAF水平差异应用SPSS13.0软件包进行统计学分析.结果:C+P组和P组患者唾液和龈沟液中PAF水平显著高于C组和H组患者(P<0.05),相关分析显示,龈沟液中PAF水平与牙周探诊深度、附着丧失水平呈正相关(P<0.05).结论:冠心病伴牙周炎患者的唾液及龈沟液中PAF水平明显升高,提示PAF的升高可能是牙周炎影响心血管疾病发生、发展的又一重要因素.

  19. Characterization of the autocrine/paracrine function of vitamin D in human gingival fibroblasts and periodontal ligament cells.

    Directory of Open Access Journals (Sweden)

    Kaining Liu

    Full Text Available BACKGROUND: We previously demonstrated that 25-hydroxyvitamin D(3, the precursor of 1α,25-dihydroxyvitamin D(3, is abundant around periodontal soft tissues. Here we investigate whether 25-hydroxyvitamin D(3 is converted to 1α,25-dihydroxyvitamin D(3 in periodontal soft tissue cells and explore the possibility of an autocrine/paracrine function of 1α,25-dihydroxyvitamin D(3 in periodontal soft tissue cells. METHODOLOGY/PRINCIPAL FINDINGS: We established primary cultures of human gingival fibroblasts and human periodontal ligament cells from 5 individual donors. We demonstrated that 1α-hydroxylase was expressed in human gingival fibroblasts and periodontal ligament cells, as was cubilin. After incubation with the 1α-hydroxylase substrate 25-hydroxyvitamin D(3, human gingival fibroblasts and periodontal ligament cells generated detectable 1α,25-dihydroxyvitamin D(3 that resulted in an up-regulation of CYP24A1 and RANKL mRNA. A specific knockdown of 1α-hydroxylase in human gingival fibroblasts and periodontal ligament cells using siRNA resulted in a significant reduction in both 1α,25-dihydroxyvitamin D(3 production and mRNA expression of CYP24A1 and RANKL. The classical renal regulators of 1α-hydroxylase (parathyroid hormone, calcium and 1α,25-dihydroxyvitamin D(3 and Porphyromonas gingivalis lipopolysaccharide did not influence 1α-hydroxylase expression significantly, however, interleukin-1β and sodium butyrate strongly induced 1α-hydroxylase expression in human gingival fibroblasts and periodontal ligament cells. CONCLUSIONS/SIGNIFICANCE: In this study, the expression, activity and functionality of 1α-hydroxylase were detected in human gingival fibroblasts and periodontal ligament cells, raising the possibility that vitamin D acts in an autocrine/paracrine manner in these cells.

  20. 牙周机械治疗对2型糖尿病患者牙周健康及龈沟液白介素6水平的影响%Effect of periodontal mechanical therapy on periodontal health and interleukin-6 levels in gingival crevicular fluid in type 2 diabetic patients with chronic periodontitis

    Institute of Scientific and Technical Information of China (English)

    谭佳玮; 项立新; 刘丽

    2011-01-01

    Objective To evaluate the effectiveness of periodontal mechanical therapy on periodontal health and interleukin-6 (IL-6) levels in gingival crevicular fluid (GCF) in type 2 diabetic patients with chronic periodontitis. Methods GCF samples were obtained with filter papers in first molar sites from 37 type 2 diabetic patients with chronic periodontitis. The randomized controlled and blinded clinical trial with a duration of six months was designed to compare effects between three groups of professional mechanical tooth cleaning (PMTC), i.e. coronal scaling (group Ⅱ) with periodontal initial therapy (group Ⅰ) and without clinical therapy (control group). The clinical periodontal index such as probing depth (PD), attachment loss (AL), modified bleeding index (mBI) and bacteria plaque index (PLI) were obtained with a Williams type periodontal probe. Laboratory examination including GCF volume, concentrations and total amounts of IL-6 in GCF were detected with ELISA method. Results Significantly decreasing trends of PD, AL, mBI, GCF volume and total amounts of IL-6 were observed in group Ⅰin all test period. The group Ⅱ had a significant reduction of PD at the third month as compared with baseline (⊿=0.36 mm, P<0.05), and the other figures showed descending trends but didn′t present statistical significances. At the end of study, the group Ⅰand Ⅱ had significant reductions of GCF volume compared with increases of control group (0.96 μ1>0.03 μ1mm>-0.20 μ1, P<0.05). Conclusions The sequential periodontal supra- and sub- gingival scaling has definitive effects on periodontal health improvement and on reducing the IL-6 level in GCF in type 2 diabetic patients.%目的 评价定期龈上洁治和龈下刮治对2型糖尿病患者牙周健康及龈沟液白介素6水平的影响.方法 采用随机、对照、盲法设计,将37例2型糖尿病伴牙周炎患者分为基础治疗组(牙周基础治疗)13例、龈上洁治组(龈上洁治)11

  1. Effects of a Nonthermal Atmospheric Pressure Plasma Jet on Human Gingival Fibroblasts for Biomedical Application

    Directory of Open Access Journals (Sweden)

    Jung-Hwan Lee

    2016-01-01

    Full Text Available Nonthermal atmospheric pressure plasma jets (APPJ have been developed and applied in biomedical research as a cancer treatment or bacterial sterilization. However, the drawback of APPJ on normal oral cells during plasma treatment and underlying cell death mechanisms have not been studied and clearly explained, although there is known to be an influence from reactive oxygen species (ROS. Hence, this study investigates whether and how a nonthermal atmospheric pressure air plasma jet kills human normal gingival cells using immortalized human gingival fibroblasts (hTERT-hNOF cells. In this study, a set of physicochemical or biological methods were used to illuminate the killing mechanisms. It was found that ROS were induced intracellularly without a breakdown of the cell wall and apoptosis was involved in cell death when an air APPJ treatment was performed on the cells directly without media; the air treatment only supported a detachment of the cells without increase of ROS. It was also revealed that a correlation between intracellular ROS concentration and cells viability existed. These results indicated that the direct air APPJ treatment possibly raises safety issue to normal tissue and thereby APPJ application in biomedical field needs more in vitro and in vivo study to optimize it.

  2. Human Gingival Integration-Free iPSCs; a Source for MSC-Like Cells

    Directory of Open Access Journals (Sweden)

    Yasuyuki Umezaki

    2015-06-01

    Full Text Available Mesenchymal stem cells (MSCs are considered a potential autologous therapy for tissue engineering. The available procedures for MSC retrieval from patients are invasive, and their limited in vitro proliferation restricts their use in the treatment of damaged tissues. Therefore, it is important to establish an alternative and safe source of MSCs. The objective of this study was to demonstrate induced pluripotent stem cell (iPSC generation from a combination of an accessible source tissue and an integration-free method; we also attempted the differentiation of iPSCs into MSC-like cells (MSLCs for future autologous tissue engineering. iPSCs were derived from human gingival tissues, which are easily accessible in the field of dentistry, via the use of non-integrating episomal plasmids. Established iPSCs expressed embryonic stem (ES cell-specific markers, as assessed by gene analysis and immunocytochemistry. Embryoid bodies and teratoma formation were formed from iPSCs, showing their capacity to differentiate into three germ layers. Furthermore, we were successful in differentiating iPSCs into MSLCs. They tested positively for their capacity of trilineage differentiation. Our results demonstrate that human gingival integration-free iPSCs, readily accessible stem cells generated using episomal plasmid vectors, are a promising source of MSLCs, which can be used in tissue regeneration.

  3. Effects of a Nonthermal Atmospheric Pressure Plasma Jet on Human Gingival Fibroblasts for Biomedical Application.

    Science.gov (United States)

    Lee, Jung-Hwan; Kim, Kyoung-Nam

    2016-01-01

    Nonthermal atmospheric pressure plasma jets (APPJ) have been developed and applied in biomedical research as a cancer treatment or bacterial sterilization. However, the drawback of APPJ on normal oral cells during plasma treatment and underlying cell death mechanisms have not been studied and clearly explained, although there is known to be an influence from reactive oxygen species (ROS). Hence, this study investigates whether and how a nonthermal atmospheric pressure air plasma jet kills human normal gingival cells using immortalized human gingival fibroblasts (hTERT-hNOF cells). In this study, a set of physicochemical or biological methods were used to illuminate the killing mechanisms. It was found that ROS were induced intracellularly without a breakdown of the cell wall and apoptosis was involved in cell death when an air APPJ treatment was performed on the cells directly without media; the air treatment only supported a detachment of the cells without increase of ROS. It was also revealed that a correlation between intracellular ROS concentration and cells viability existed. These results indicated that the direct air APPJ treatment possibly raises safety issue to normal tissue and thereby APPJ application in biomedical field needs more in vitro and in vivo study to optimize it.

  4. 镍铬、金合金烤瓷牙修复后不同时期龈沟液IL-8含量水平探讨%To explore the different periods of gingival crevicular fluid IL levels of-8 Cr, gold alloy porcelain teeth

    Institute of Scientific and Technical Information of China (English)

    孙国能

    2016-01-01

    Objective To investigate the effect of gold alloy and Ni Cr porcelain dental restoration on periodontal tissue. Methods in our hospital in 2011 during the May 5 to June 2012 80 cases of anterior teeth defect patients. According to the dif-ferent crown materials within the repair will be the group of gold alloy, Ni Cr alloy was observed in different periods of aspartate aminotransferase (AST) and IL-8 levels, gingival crevicular fluid amount (GCF), phosphorus, alkaline acid (ALP). Results after treatment, 1 weeks, two kinds of alloy porcelain compared with the previous repair in gingival crevicular fluid volume, to-tal IL-8, with statistical significance;3, 6 months after the repair, the gold alloy group the index decreased significantly, to 6 months and gradually returned to the level before the repair. Ni Cr alloy group the index at 6 months in the repair and restoration of the former comparison was statistically significant, gold alloy group in the repair of the 3 and 6 months, the index was signifi-cantly lower than that of Ni Cr alloy group, the difference was significant;the content of IL-8, two kinds of alloy porcelain only significant differences in the repair of 3 months, repair at 6 months, two kinds of metal ceramic were restored to the level before the repair;Ni Cr alloy group repair at 6 months of AST, ALP and repair before there was significant difference, and gold The AST level was significantly increased in the alloy group at 6 months, and the difference was not significant between the gold al-loy group and the healthy teeth at 6 months after the repair of the ALP. Conclusion the irritation of the gold alloy to the gums is small, and it is an ideal material for the porcelain fused to metal crown.%目的:探讨金合金、镍铬烤瓷牙修复对牙周组织的影响。方法:选取我院2011年5月~2012年5月期间的80例前牙缺损患者,根据修复内冠材料不同将其分为金合金组、镍铬合金组,观察不同时期天

  5. Tooth correction using self-ligating bracketversusconventional bracket appliance:expression of interleukin 1beta and tumor necrosis factor alpha in gingival crevicular fluid after correction%自锁托槽与传统托槽矫治器矫正后龈沟液内白细胞介素1β和肿瘤坏死因子α的表达

    Institute of Scientific and Technical Information of China (English)

    崔占琴; 李文静; 郁焕兵

    2015-01-01

    背景:在临床正畸矫正过程中,自锁托槽较传统托槽更加有利于牙周健康的维护,但以往的研究多局限于牙周临床指数和牙周致病菌方面,炎性因子是否参与其中尚未见相关报道。目的:对比自锁托槽与传统托槽矫治器矫治前后龈沟液内白细胞介素1β和肿瘤坏死因子α表达水平的变化。方法:选择需做固定矫治且牙周健康的38例患者为受试对象,其中男20例,女18例,年龄11-25岁,按照随机数字表法分为对照组和试验组,每组19例,分别采用传统托槽矫治器与自锁托槽矫治器进行矫治。在矫治前、矫治后4,8,12周检测龈沟液量及其中白细胞介素1β和肿瘤坏死因子α表达水平。结果与结论:两组矫治后的龈沟液量、白细胞介素1β和肿瘤坏死因子α水平均高于矫治前(P<0.05);试验组矫治后不同时间点的龈沟液量、白细胞介素1β和肿瘤坏死因子α表达水平低于对照组(P<0.05),表明自锁托槽矫治器更加有利于保护牙周组织的健康。%BACKGROUND:During clinical orthodontic correction, the self-ligating bracket is more helpful to maintain the periodontal healthy than the traditional bracket, but previous studies mainly focused on the clinical periodontal index and periodontal pathogens, and whether inflammatory cytokines are involved has not been reported. OBJECTIVE:To analyze the changes of the expression levels of interleukin 1β and tumor necrosis factor α in gingival crevicular fluid before and after the correction using self-ligating bracket and conventional bracket appliance. METHODS:Totaly 38 orthodontic patients without periodontal disease (including 20 males and 18 females; aged 11-25 years) were included in this study. Al the patients were randomly divided into control and test groups (n=19/group) and subjected to orthodontic correction using self-ligating bracket and conventional bracket appliance, respectively

  6. The effect of exposure duration of self etch dentin bonding on the toxicity of human gingival fibroblast of cell culture

    Directory of Open Access Journals (Sweden)

    Sri Lestari

    2008-06-01

    Full Text Available Self etch dentin bonding created to make light easily activate the application of composite resin on tooth surface. The monomer content has acid effect that could irritate tooth pulp. The purpose of this study was to evaluate the effect of light exposure duration of self etch dentin bonding on toxicity of human gingival fibroblast of cell culture by MTT assay. Self etch dentin bonding was used as on experimental unit and the sample was exposed by visible light curing in different duration: 10, 20, 30 seconds and immerged in artificial saliva in pH 7 for 24 hours. 100 µl artificial saliva was exposed to human gingival fibroblast of cell culture 20.000 cells/100 µl RPMI for 24 hours. Toxicity was evaluated by MTT assay, optical density was measured using 550 nm spectrophotometer. The data was analyzed using Kruskal Wallis in 5% degree of significance. The result showed that increasing exposure duration (10, 20, 30 seconds of self etch dentin bonding will reduce the toxicity of human gingival fibroblast of cell culture. It is concluded that 30 seconds-exposure of self etch dentin bonding will reduce the toxicity of human gingival fibroblast of cell culture.

  7. Expression and function of connexin 43 in human gingival wound healing and fibroblasts.

    Directory of Open Access Journals (Sweden)

    Rana Tarzemany

    Full Text Available Connexins (C×s are a family of transmembrane proteins that form hemichannels and gap junctions (GJs on the cell membranes, and transfer small signaling molecules between the cytoplasm and extracellular space and between connecting cells, respectively. Among C×s, suppressing C×43 expression or function promotes skin wound closure and granulation tissue formation, and may alleviate scarring, but the mechanisms are not well understood. Oral mucosal gingiva is characterized by faster wound closure and scarless wound healing outcome as compared to skin wounds. Therefore, we hypothesized that C×43 function is down regulated during human gingival wound healing, which in fibroblasts promotes expression of genes conducive for fast and scarless wound healing. Cultured gingival fibroblasts expressed C×43 as their major connexin. Immunostaining of unwounded human gingiva showed that C×43 was abundantly present in the epithelium, and in connective tissue formed large C×43 plaques in fibroblasts. At the early stages of wound healing, C×43 was strongly down regulated in wound epithelial cells and fibroblasts, returning to the level of normal tissue by day 60 post-wounding. Blocking of C×43 function by C×43 mimetic peptide Gap27 suppressed GJ-mediated dye transfer, promoted migration, and caused significant changes in the expression of wound healing-associated genes in gingival fibroblasts. In particular, out of 54 genes analyzed, several MMPs and TGF-β1, involved in regulation of inflammation and extracellular matrix (ECM turnover, and VEGF-A, involved in angiogenesis, were significantly upregulated while pro-fibrotic ECM molecules, including Collagen type I, and cell contractility-related molecules were significantly down regulated. These responses involved MAPK, GSK3α/β and TGF-β signaling pathways, and AP1 and SP1 transcription factors. Thus, suppressed function of C×43 in fibroblasts promotes their migration, and regulates expression of

  8. Apoptotic and necrotic influence of dental resin polymerization initiators in human gingival fibroblast cultures.

    Science.gov (United States)

    Masuki, Kouhei; Nomura, Yuji; Bhawal, Ujjal Kumar; Sawajiri, Masahiko; Hirata, Isao; Nahara, Yukinori; Okazaki, Masayuki

    2007-11-01

    The aim of this study was to examine the apoptotic and necrotic influence of four dental resin polymerization initiators--namely benzoyl peroxide (BPO), camphorquinone (CQ), dimethylaminoethyl methacrylate (DMAEMA), and dimethyl-para-toluidine (DMPT)--on human gingival fibroblast (HGF) cells. To this end, the growth inhibition of HGF cells with 1 mM BPO, CQ, and DMAEMA, and 500 microM DMPT was evaluated using Cell Counting Kit-8. Then, cell cycle analysis by flow cytometry was used to assess propidium iodide-stained cells (distribution of cells in G0/G1, S, G2/M phases). All four dental resin polymerization initiators induced G0/G1 cell cycle arrest. As for the patterns of cell death (necrosis and/or apoptosis), they were analyzed using Annexin V-FITC/PI staining with flow cytometry. All four dental resin polymerization initiators most likely induced necrosis.

  9. Gingival tissue transcriptomes in experimental gingivitis

    Science.gov (United States)

    Jönsson, Daniel; Ramberg, Per; Demmer, Ryan T.; Kebschull, Moritz; Dahlén, Gunnar; Papapanou, Panos N.

    2012-01-01

    Aims We investigated the sequential gene expression in the gingiva during the induction and resolution of experimental gingivitis. Methods Twenty periodontally and systemically healthy non-smoking volunteers participated in a 3-week experimental gingivitis protocol, followed by debridement and 2-week regular plaque control. We recorded clinical indices and harvested gingival tissue samples from 4 interproximal palatal sites in half of the participants at baseline, Day 7, 14 and 21 (‘induction phase’), and at day 21, 25, 30 and 35 in the other half (‘resolution phase’). RNA was extracted, amplified, reversed transcribed, amplified, labeled and hybridized with Affymetrix Human Genome U133Plus2.0 microarrays. Paired t-tests compared gene expression changes between consecutive time points. Gene ontology analyses summarized the expression patterns into biologically relevant categories. Results The median gingival index was 0 at baseline, 2 at Day 21 and 1 at Day 35. Differential gene regulation peaked during the third week of induction and the first four days of resolution. Leukocyte transmigration, cell adhesion and antigen processing/presentation were the top differentially regulated pathways. Conclusions Transcriptomic studies enhance our understanding of the pathobiology of the reversible inflammatory gingival lesion and provide a detailed account of the dynamic tissue responses during induction and resolution of experimental gingivitis. PMID:21501207

  10. Effects of titania nanotubes with or without bovine serum albumin loaded on human gingival fibroblasts

    Directory of Open Access Journals (Sweden)

    Liu X

    2014-03-01

    Full Text Available Xiangning Liu,1,* Xiaosong Zhou,2,* Shaobing Li,3 Renfa Lai,1 Zhiying Zhou,1 Ye Zhang,1 Lei Zhou3 1The First Affiliated Hospital of Jinan University, Guangzhou, 2Chemistry Science and Technology School, Zhanjiang Normal University, Zhanjiang, 3Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, People's Republic of China *These authors contributed equally to this work Abstract: Modifying the surface of the transmucosal area is a key research area because this process positively affects the three functions of implants: attachment to soft tissue, inhibiting bacterial biofilm adhesion, and the preservation of the crestal bone. To exploit the potential of titania nanotube arrays (TNTs with or without using bovine serum albumin (BSA to modify the surface of a dental implant in contact with the transmucosal area, BSA was loaded into TNTs that were fabricated by anodizing Ti sheets; the physical characteristics of these arrays, including their morphology, chemical composition, surface roughness, contact angle, and surface free energy (SFE, were assessed. The effect of Ti surfaces with TNTs or TNTs-BSA on human gingival fibroblasts (HGFs was determined by analyzing cell morphology, early adhesion, proliferation, type I collagen (COL-1 gene expression, and the extracellular secretion of COL-1. The results indicate that early HGF adhesion and spreading behavior is positively correlated with surface characteristics, including hydrophilicity, SFE, and surface roughness. Additionally, TNT surfaces not only promoted early HGF adhesion, but also promoted COL-1 secretion. BSA-loaded TNT surfaces promoted early HGF adhesion, while suppressing late proliferation and COL-1 secretion. Therefore, TNT-modified smooth surfaces are expected to be applicable for uses involving the transmucosal area. Further study is required to determine whether BSA-loaded TNT surfaces actually affect closed loop formation of connective tissue because

  11. The adiponectin level in gingival crevicular fluid in patients of chronic periodontitis with diabetes mellitus type 2%慢性牙周炎伴2型糖尿病患者龈沟液中脂联素水平的检测及意义

    Institute of Scientific and Technical Information of China (English)

    刘道洲; 吴万红; 姜慧; 张帆; 黄萍

    2016-01-01

    Objective:To examine the adiponectin level in gingival crevicular fluid(GCF)in patients of chronic periodontitis with dia-betes mellitus type 2.Methods:20 patients of diabetes mellitus type 2 with chronic periodontitis(DM&CP),20 of periodontitis(CP) and 20 health subjects(H)were included.The periodontal indexes (SBI,PLI,PD and AL)were measured,GCF samples were quan-tified by periotron 8000,the adiponectin content in GCF was tested by adiponectin ELISA kit.The relationship between the adiponectin level in GCF and the periodontal indexes of the DM&CP patients was analyzed statistically.Results:The adiponectin level in GCF in group DM&CP was significantly lower than that in the other 2 groups(P 0.05).Conclusion:Decrease of adiponectin in GCF may play a role in the development of DM&CP.%目的:探究牙周炎伴2型糖尿病患者龈沟液中脂联素水平及意义。方法:纳入慢性牙周炎伴糖尿病患者(DM&CP)、慢性牙周炎患者(CP)、健康对照者(H)各20例,记录其临床指标(SBI、PLI、PD 和 AL),并收集龈沟液(GCF)样本,用龈沟液测量仪 periotron8000对 GCF 进行定量,用脂联素 ELISA 试剂盒检测样本中脂联素含量,比较组间检测指标的差异以及临床指标与 GCF 中脂联素水平的相关性。结果:DM&CP 组 GCF 中脂联素水平显著低于其他2组(P <0.05),CP 患者龈沟液脂联素水平与牙周健康组相比无统计学差异(P >0.05)。GCF 中脂联素水平与临床指标 PD 值、AL 值有负相关性(P <0.05),与 SBI、PLI 无明显相关(P >0.05)。结论:龈沟液中脂联素水平降低可能与 DM&CP 发生发展有关。

  12. The correlation of OPG/RANKL expression level of gingival crevicular fluid to sulcus bleeding index in patients with chronic periodontitis%龈沟液 OPG/RANKL 表达与慢性牙周炎患者龈沟出血指数的相关性

    Institute of Scientific and Technical Information of China (English)

    吕旭君

    2015-01-01

    目的:分析慢性牙周炎患者龈沟液护骨素( osteoprotegerin,OPG)和核因子κB受体活化因子配体( receptor ac-tivator of nuclear factor-KB ligand,RANKL)表达与其龈沟出血指数的关系。方法以吸潮纸尖法,取37例慢性牙周炎患者(观察组)和同期入院体检的31例健康志愿者(对照组)的龈沟液。采用免疫荧光试验分析OPG和RANKL的表达强度;并以简单线性回归法测试OPG/RANKL表达与龈沟出血指数的相关性。结果与对照组比较,观察组患者OPG和OPG/RANKL表达强度明显偏低,RANKL表达强度明显偏高,且各指标的组间差异均有统计学意义( P<0.001)。经线性回归分析,观察组患者的 OPG/RANKL 的表达情况与其出血指数呈显著负相关( y =-3.7755x+3.6212,R2=0.6651,P<0.05)。结论 OPG/RANKL/RANK系统参与了慢性牙周炎的发生和发展,可作为衡量其炎症性出血程度的客观指标。%Objective To examine the relationship between the level of osteoprotegerin ( OPG) and receptor activator of nuclear factor-KB ligand ( RANKL) expression and sulcus bleeding index.Method The gingival crevicular fluid of 37 patients with chronic periodontitis ( chosen as the experimental group ) and 31 healthy volunteers were abstracted with moisture absorption paper point method.Their OPG and RANKL expression level were detected with immunofluorescence test and the correlation of OPG/RANKL expression and sulcus bleeding index were analyzed with simple linear regression. Findings Compared with the control group, the OPG and OPG/RANKL expression level were lower significantly and the RANKL expression level were higher significantly (P<0.001).Negative correlation was found between the OPG/RANKL expression level and sulcus bleeding index (y=-3.7755x +3.6212,R2 =0.6651,P<0.05).Conclusion OPG/RANKL/RANK system is involved in the occurrence and development of chronic periodontitis

  13. 龈沟液中IL-1β与2型糖尿病和牙周病相关性的研究%The research of correlation among IL-1β in gingival crevicular fluid and type 2 diabetes, periodontal diseases

    Institute of Scientific and Technical Information of China (English)

    张兰; 丁岩; 邓晓红; 宋健玲

    2013-01-01

    Objective:To compare the different level of IL-1β in gingival crevicular fluid(GCF)among type 2 diabetes mellitus patients (DM), type 2 diabetes mellitus with periodontal diseases patients (DM&AP) and periodontal diseases patients (AP), we investigated the relationship between concetration of HbAlc and IL-1β in DM and DM&AP. Method: GCF were collected by filter strips.DM,DM & AP.AP patients and normal control group of 30 patients in each were included in this study. Their IL-1β were measured by ELASA, HbAlc in DM and DM&AP were detected by High Performance Liquid Chromatography. Result:The diabetics, diabetics with periodontal diseases and periodontal diseases patients had higher GCF and IL-1β than normal control group (P <0.05). The diabetics with periodontal diseases and periodontal diseases patients had higher GCF than the diabetics group (P <0.05). The diabetics with periodontal diseases had higher IL-1β than the diabetics group and periodontal diseases patients (P <0.05). Conclusion: The diabetics and diabetics with periodontal diseases patients have higher IL-ip than normal. Long-term hyperglycemia causes diabetic patients susceptible to periodontal disease. So diabetic patients should highly concerned about periodontal health as they control blood suger.%目的:比较2型糖尿病患者(DM)、2型糖尿病伴牙周病患者(DM&AP)及牙周病患者(AP)龈沟液(GCF)中的白细胞介素-1β(IL-1β)水平,探讨DM和DM&AP糖化血红蛋白(HbAlc)浓度与IL-1β的相关性.方法:使用滤纸条法采集GCF,DM、DM&AP、AP患者及正常对照组各30例;ELISA法测定GCF中IL-1β含量;运用高效液相层析法测定DM和DM&AP的糖化血红蛋白水平.结果:DM、DM&AP、AP的GCF量和IL-1β量均高于正常对照组(P<0.05);DM&AP、AP组GCF量显著高于DM组(P <0.05);DM&AP组IL-1β含量高于DM组和AP组(P<0.05).结论:糖尿病患者和糖尿病伴牙周病患者IL-1β均高于正常人,长期的高血糖会使糖尿病患者

  14. Ⅱ型糖尿病合并牙周病患者龈沟液中肿瘤坏死因子α水平和意义%Measurement and Analysis of TNF-α in Gingival Crevicular Fluid from Type 2 Diabetes Complicated with Periodontal Diseases Patients

    Institute of Scientific and Technical Information of China (English)

    张兰; 丁岩

    2012-01-01

    Objective: To compare the TNF-α level in gingival crevicular fluid (GCF) from periodonlal diseases in type 2 diabetes mellitus with which from systemically healthy person and the relationship between TNF-α concentration and metabolic condition. Methods: Thirty diabetics complicated with periodontal diseases patients and perio-dontal diseases and thirty systemically healthy persons were included in this study. Their TNF-α was measured by radioimmunoassay. and HbAlc levels of diabetic patients were measured by high-performance liquid chromatogra-phy. Results: The diabetics with periodontal diseases and patients with periodontal diseases had higher GCF and GCF-TNF-α as compared to the control group(P<0. 01). The diabetics with periodontal diseases had higher GCF-TNF-α as compared to the periodontal diseases group (P<0. 01). The levels of TNF-α of diabetics with periodontal diseases did not correlate with HbAlc levels. Conclusion: The TNF-α level was controlled by systemic factor. The high TNF-α contents in diabetics may be a consequence of a systemic abnormal response and a more severe periodontal disease may thus be resulted.%目的:比较Ⅱ型糖尿病合并牙周病患者、牙周病患者与健康者龈沟液中肿瘤坏死因子-α(TNF-α)含量和龈沟液量,以及Ⅱ型糖尿病合并牙周病患者龈沟液中TNF-α量与糖代谢状况的关系.方法:使用滤纸条法采集龈沟液,Ⅱ型糖尿病合并牙周病患者、牙周病患者和全身健康者各30例;放射免疫法测定龈沟液中TNF-α含量;运用高效液相层析法测定Ⅱ型糖尿病合并牙周病患者的糖化血红蛋白水平.结果:糖尿病合并牙周病组和牙周病组龈沟液量及龈沟液TNF-α量均显著高于正常对照组(P<0.01),糖尿病合并牙周病组龈沟液TNF-α量显著高于牙周病组(P<0.01).糖尿病合并牙周病组龈沟液中TNF-α含量与糖化血红蛋白(HbA1c)水平无显著直线相关性.结论:龈沟液中TNF-α含量

  15. 3种金属烤瓷冠修复后龈沟液中白细胞介素-8水平动态测定%Determination of the dynamic levels of interleukin-8 in gingival crevicular fluid of porcelain teeth with three different alloys

    Institute of Scientific and Technical Information of China (English)

    许卫星; 苏俭生

    2012-01-01

    目的 探讨镍铬合金、钴铬合金、金合金3组金属烤瓷冠修复前后不同时期龈沟液(GCF)内白细胞介素-8(IL-8)水平变化,了解这3种金属烤瓷冠在不同时期对牙龈的刺激程度.方法 临床随机选择3种金属烤瓷冠修复患者共45例,每组各15例,测定在修复前以及修复后1、3、6个月GCF量,并采用双抗体夹心酶联免疫吸附法检测同期GCF中IL-8总量、IL-8含量.结果 镍铬合金烤瓷冠在修复1、3、6个月后GCF量、IL-8总量与修复前相比差异均有统计学意义(P<0.05).钴铬合金、金合金烤瓷冠在修复3个月后回复到修复前水平.3种金属烤瓷冠IL-8含量在不同时期差异均无统计学意义.结论 IL-8参与炎症的免疫调节,可作为评价不同金属烤瓷冠对牙龈组织刺激程度的指标,不同合金烤瓷冠内冠材料对牙龈存在不同的长期刺激,临床金属烤瓷冠修复时对内冠材料的选择应予以关注.%Objective To study the interleukin-8 (IL-8) levels in gingival crevicular fluid (GCF) of porcelain teeth coated with Ni-Cr, Co-Cr or gold alloy at different time periods, and to uncover the degree of stimulation by these alloys on gingiva at different time periods. Methods 45 cases of porcelain teeth coated with Ni-Cr, Co-Cr or gold alloy were selected randomly, with 15 cases in each group. Sandwich enzyme-linked immunosorbent assay (ELISA) was used to determine the concentration and total amount of IL-8 in GCF. The assay was done before treatment, as well as at 1, 3 and 6 months post-treatment. Results The total amounts of IL-8 and GCF volume in the Ni-Cr alloy coated porcelain teeth were higher in different time period than those before treatment (P<0.05). However, the IL-8 levels in Co-Cr and gold alloy coated porcelain teeth returned to pre-dental restoration after 3 months. Otherwise, the levels of IL-8 concentration in GCF showed no significant difference among the three different alloys coating at different

  16. RELATION BETWEEN ALKALINE PHOSPHATASE IN GINGIGVAL CREVICULAR FLUID OF IMPLANT TEETH AND THE CURING RESULT

    Institute of Scientific and Technical Information of China (English)

    周正; 周坚; 邹石莹; 吴效民

    2001-01-01

    Objective. To discover the relation between alkaline phosphatase (ALP) in gingival crevicular fluid (GCF)of implant teeth aad the curing results.Methods. We measured the ALP level in GCF among 56 cases of implant teeth which included 2 failed cas-es, 5 cases with bad oral hygiene and gingivitis, and compared it with that in the normal group composed of 10persons.Results. The ALP levels in normal group and success implant group showed no difference. The ALP levelsin normal group and success with gingivitis group showed obvious difference. The ALP levels of the 2 failed cas-es are the highest of all.Conclusions. The ALP level in GCF is an important index in evaluating the curing result of the implantteeth.``

  17. Long-term exposure of human gingival fibroblasts to cigarette smoke condensate reduces cell growth by modulating Bax, caspase-3 and p53 expression.

    Science.gov (United States)

    Alamri, A; Semlali, A; Jacques, É; Alanazi, M; Zakrzewski, A; Chmielewski, W; Rouabhia, M

    2015-08-01

    Smoking cigarettes increases the risk of oral tissue damage leading to periodontal disease. Gingival fibroblasts, the predominant cell type inhabiting gingival connective tissue, play a critical role in remodeling and maintaining gingival structure. The objective of this study was to investigate the effect of long-term exposure to cigarette smoke on human gingival fibroblast survival/apoptosis and the molecular pathways involved in these cell responses. Human gingival fibroblasts were extracted from healthy non-smokers and cultured in the presence of cigarette smoke condensate (CSC). At the end of each time point, cell growth was evaluated by means of MTT assay. Apoptotic and necrotic gene's expression was investigated by polymerase chain reaction array and by annexin V/propidium iodide staining and cell cycle assays. Western blot was used to investigate Bax and p53 proteins. These tests were supported by caspase 3 activity analyses. High levels of CSC decreased cell growth and deregulated cell cycle progression by increasing the G(0)/G(1) and reducing the S and G(2)/M phases of the gingival fibroblasts. Polymerase chain reaction arrays revealed the activation of several apoptotic genes by CSC, including TNF receptors, caspases, Bax and p53. This was supported by increases in the Bax and p53 protein levels as well as by an elevated activity of caspase-3 in the CSC-exposed cells. Immunofluorescence staining demonstrated that both Bax and caspase-3 displayed a cytosolic and mitochondrial distribution in the CSC-exposed gingival fibroblasts, compared to controls. The damaging effect of CSC on gingival fibroblast growth was also supported by the decrease in interleukin 6 and 8 secretion by the gingival fibroblasts. These results suggest that CSC may contribute to deregulating fibroblast functions. This can compromise fibroblast-epithelial cell interactions, which ultimately increases the risk of gingival tissue damage and the onset of periodontitis. © 2014 John Wiley

  18. Anti-inflammatory changes of gene expression by Artemisia iwayomogi in the LPS-stimulated human gingival fibroblast: microarray analysis.

    Science.gov (United States)

    Choi, Yeong-Gon; Yeo, Sujung; Kim, Sung-Hoon; Lim, Sabina

    2012-03-01

    The leaves and stems of Asteraceae Artemisia iwayomogi (Ai) for a long time have been known to inhibit inflammatory cytokine production and allergic reactions, and have been used to treat liver diseases. It needs to be elucidated in terms of global gene expression whether Ai has an influence as an anti-inflammatory agent on the cultured human gingival fibroblast stimulated with lipopolysaccharide (LPS). This study investigated the anti-inflammatory changes of the genes by Ai using the Affymetrix genechip human gene 1.0 ST array when the cultured human gingival fibroblast was treated with LPS. It was observed that the inflammation- and immune response-related genes were activated by LPS challenge in the cultured human gingival fibroblast. The array analysis showed that 65 of the 344 genes up-regulated by LPS stimulation, when compared to the control, were down-regulated by the Ai treatment. A number of inflammation- and immune response-related genes of the 65 genes were found. In addition, 78 of the 164 genes down-regulated by the LPS, when compared to the control, were up-regulated by the Ai treatment. The regulatory patterns of the representative genes were correlated with the real-time RT-PCR analysis. The Ai extract and its specific components, scopolin and scopoletin, significantly hindered the production of inflammatory mediators such as IL-6, TNF-α and nitrite in the LPS-challenged fibroblast. This study suggests that Ai can comprehensively inhibit the activation of the inflammation- and immune response-related genes and the inflammatory mediators in the human gingival fibroblast.

  19. Biocompatibility of Mineral Trioxide Aggregate with TiO2 Nanoparticles on Human Gingival Fibroblasts

    Science.gov (United States)

    Samiei, Mohammad; Aghazadeh, Marzieh; Divband, Baharak; Akbarzadeh, Farzaneh

    2017-01-01

    Background The New compositions of white mineral trioxide aggregate (WMTA) or use of various additives like nanoparticles might affect MTA’s ideal characteristics This study was performed to evaluate the cytotoxicity of WMTA and WMTA with Titanium dioxide (TiO2) nanoparticles (1% weight ratio) at different storage times after mixing on human gingival fibroblasts (HGFs). Material and Methods HGFs were obtained from the attached gingiva of human premolars. HGFs were cultured in Dulbecco’s Modified Eagle medium, supplemented with 10% fetal calf serum, penicillin and streptomycin. The cells were exposed to WMTA (groups 1 and 2) and WMTA+TiO2 (groups 3 and 4). The fifth and sixth groups served as controls. Each group contained 15 wells. After 24h (groups 1, 3 and 5) and 48 h (groups 2, 4 and 6) of exposure, HGF viability was determined by Mosmann’s tetrazolium toxicity (MTT) assay. Statistical analysis of the data was performed by using one-way analysis of variance and Tukey post hoc test, with significance of p 0.05). Conclusions Under the limitations of the present study, incorporation of TiO2 nanoparticles into MTA at 1 wt% had no negative effect on its biocompatibility. Key words:Cytotoxicity, fibroblast, MTA, MTT assay, nanoparticle, TiO2. PMID:28210432

  20. Inhibition of the differentiation of monocyte-derived dendritic cells by human gingival fibroblasts.

    Directory of Open Access Journals (Sweden)

    Sylvie Séguier

    Full Text Available We investigated whether gingival fibroblasts (GFs can modulate the differentiation and/or maturation of monocyte-derived dendritic cells (DCs and analyzed soluble factors that may be involved in this immune modulation. Experiments were performed using human monocytes in co-culture with human GFs in Transwell® chambers or using monocyte cultures treated with conditioned media (CM from GFs of four donors. The four CM and supernatants from cell culture were assayed by ELISA for cytokines involved in the differentiation of dendritic cells, such as IL-6, VEGF, TGFβ1, IL-13 and IL-10. The maturation of monocyte-derived DCs induced by LPS in presence of CM was also studied. Cell surface phenotype markers were analyzed by flow cytometry. In co-cultures, GFs inhibited the differentiation of monocyte-derived DCs and the strength of this blockade correlated with the GF/monocyte ratio. Conditioned media from GFs showed similar effects, suggesting the involvement of soluble factors produced by GFs. This inhibition was associated with a lower stimulatory activity in MLR of DCs generated with GFs or its CM. Neutralizing antibodies against IL-6 and VEGF significantly (P<0.05 inhibited the inhibitory effect of CM on the differentiation of monocytes-derived DCs and in a dose dependent manner. Our data suggest that IL-6 is the main factor responsible for the inhibition of DCs differentiation mediated by GFs but that VEGF is also involved and constitutes an additional mechanism.

  1. Growth inhibitory effects of endotoxins from Bacteroides gingivalis and intermedius on human gingival fibroblasts in vitro

    Energy Technology Data Exchange (ETDEWEB)

    Layman, D.L.; Diedrich, D.L.

    1987-06-01

    Purified endotoxin or lipopolysaccharide from Bacteroides gingivalis and Bacteroides intermedius caused a similar dose-dependent inhibition of growth of cultured human gingival fibroblasts as determined by /sup 3/H-thymidine incorporation and direct cell count. Approximately 200 micrograms/ml endotoxin caused a 50% reduction in /sup 3/H-thymidine uptake of logarithmically growing cells. Inhibition of growth was similar in cultures of fibroblasts derived from either healthy or diseased human gingiva. When examining the change in cell number with time of exposure in culture, the rate of proliferation was significantly suppressed during the logarithmic phase of growth. However, the cells recovered so that the rate of proliferation, although reduced, was sufficient to produce a cell density similar to the control cells with prolonged culture. The endotoxins were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The profiles of the Bacteroides endotoxins were different. B. gingivalis endotoxin showed a wide range of distinct bands indicating a heterogeneous distribution of molecular species. Endotoxin from B. intermedius exhibited a few discrete low molecular weight bands, but the majority of the lipopolysaccharides electrophoresed as a diffuse band of high molecular weight material. The apparent heterogeneity of the two Bacteroides endotoxins and the similarity in growth inhibitory capacity suggest that growth inhibitory effects of these substances cannot be attributed to any polysaccharide species of endotoxin.

  2. Evaluation of resorbable membrane in treatment of human gingival isolated buccal recession

    Directory of Open Access Journals (Sweden)

    Sumit Narang

    2011-01-01

    Conclusion: Resorbable membrane is a versatile treatment modality for coverage of isolated buccal gingival recession. Although membrane exposure occurred in four patients, it did not interfere with post operative healing.

  3. Antimicrobial effects and human gingival biocompatibility of hydroxyapatite sol-gel coatings.

    Science.gov (United States)

    Chung, Ren-Jei; Hsieh, Ming-Fa; Huang, Chine-Wen; Perng, Li-Hsiang; Wen, Hsiao-Wei; Chin, Tsung-Shune

    2006-01-01

    The sol-gel method was employed to synthesize hydroxyapatite (HAp) coatings modified with Ag or Zn ions onto Ti-6Al-4V substrate. A bacterial strain Streptococcus mutans (S. mutans) and a human gingival fibroblast (HGF-1) cell line were used to investigate the antimicrobial effect and biocompatibility, respectively. HAp coatings containing 100 ppm Ag(+) ions suppressed the growth of S. mutans. An apparent inhibition zone around the HAp coating was further observed at Ag(+) concentration up to 10,000 ppm. However, for coatings containing Zn(2+) ions, a clear inhibition zone was observed at Zn(2+) concentration of 10,000 ppm. Nevertheless, the results of HGF-1 cultivation demonstrated that the Zn(2+)-modified HAp coatings exhibited better attachment and spread of HGF-1 than did the Ag(+)-modified coatings. Zn(2+) modified HAp coatings also increased the plating efficiency of HGF-1 cells. The cytotoxicity associated with the addition of Ag and the cell-conductive capacity associated with the addition of Zn are proportional to the added concentration, from 100 to 10,000 ppm. The dosages of both Ag(+) and Zn(2+) ions that should be added to HAp coatings were considered to prevent infection and improve biocompatibility. The results of this study ensure that HAp coatings modified with a moderate amount of Ag/Zn efficiently resist microorganisms and improve biocompatibility.

  4. Characterization of Human Gingival Fibroblasts on Zirconia Surfaces Containing Niobium Oxide

    Directory of Open Access Journals (Sweden)

    Young-Dan Cho

    2015-09-01

    Full Text Available It was indicated that tetragonal zirconia polycrystal (TZP containing yttria (Y2O3 and niobium oxide (Nb2O5 ((Y,Nb-TZP could be an adequate dental material to be used at esthetically important sites. The (Y,Nb-TZP was also proved to possess its osteogenic potential comparable with those conventional dental implant material, titanium (Ti. The objective of the current study was to characterize cellular response of human gingival fibroblasts (HGFs to smooth and rough surfaces of the (Y,Nb-TZP disc, which were obtained by polishing and sandblasting, respectively. Various microscopic, biochemical, and molecular techniques were used to investigate the disc surfaces and cellular responses for the experimental (Y,Nb-TZP and the comparing Ti groups. Sandblasted rough (Y,Nb-TZP (Zir-R discs had the highest surface roughness. HGFs cultured on polished (Y,Nb-TZP (Zir showed a rounded cell morphology and light spreading at 6 h after seeding and its proliferation rate significantly increased during seven days of culture compared to other surfaces. The mRNA expressions of type I collagen, integrin α2 and β1 were significantly stimulated for the Zir group at 24 h after seeding. The current findings, combined with the previous results, indicate that (Y,Nb-TZP provides appropriate surface condition for osseointegration at the fixture level and for peri-implant mucosal sealing at the abutment level producing a suitable candidate for dental implantation with an expected favorable clinical outcome.

  5. The effect of laser-treated titanium surface on human gingival fibroblast behavior.

    Science.gov (United States)

    Baltriukienė, D; Sabaliauskas, V; Balčiūnas, E; Melninkaitis, A; Liutkevičius, E; Bukelskienė, V; Rutkūnas, V

    2014-03-01

    Surface modification, as a means of enhancing soft tissue integration in titanium would have significant advantages including less marginal bone resorption, predictable esthetic outcome, improved soft tissue stability, and seal against bacterial leakage. The aim of this study was to evaluate the effects of laser-roughened titanium surfaces on human gingival fibroblast (HGF) viability, proliferation, and adhesion. Titanium discs were ablated with impulse laser in four different patterns. Polished and sand-blasted titanium discs were used as control groups. Specimen surface properties were determined using optical profilometry and scanning electron microscopy. HGF behavior on modified surfaces was analyzed using cell adhesion, viability, proliferation, and ELISA assays. Results suggested that modified Ti surfaces did not affect the viability of HGFs and improved adhesion was measured in laser treatment groups after 24 h. However, proliferation study showed that the adsorbance of fibroblast cells after 72 h cultured on polished titanium was higher and comparable with that of control cells. As for focal adhesion kinase (FAK), cells grown on laser modified surfaces had higher expression of FAK as compared with polished titanium. In conclusion, tested laser-treated surfaces seem to favor HGF adhesion. There were no significant differences between different laser treatment groups. Copyright © 2013 Society of Plastics Engineers.

  6. Enhanced Biological Behavior of In Vitro Human Gingival Fibroblasts on Cold Plasma-Treated Zirconia.

    Directory of Open Access Journals (Sweden)

    Miao Zheng

    Full Text Available To evaluate whether atmospheric-pressure dielectric-barrier-discharge plasma treatment of zirconia enhances its biocompatibility with human gingival fibroblasts.The zirconia disks were divided into four groups and treated using helium atmospheric-pressure dielectric-barrier-discharge plasmas for 30, 60 or 90 s or left untreated. The surface morphology, wettability and chemical elements were analyzed. Fibroblasts density, morphology, morphometry and attachment-related genes expression were measured at different time points from 3 to 72 h.After plasma treatment, the surface morphology and roughness remained the same, while the contact angle decreased from 78.31° to 43.71°, and the surface C/O ratio decreased from 3.17 to 0.89. The surficial areas and perimeters of HGFs were increased two-fold in the treated groups at 3 h. Fibroblasts density increased on treated disks at all time points, especially the ones treated for 60 s. Attachment-related genes in the groups treated for 30 and 60 s were significantly higher at 3 and 24 h.The helium atmospheric-pressure dielectric-barrier-discharge plasma treatment enhances the biological behavior of fibroblasts on zirconia by increasing the expression of attachment-related genes within 24 h and promoting the cell density during longer culture times. Wettability of zirconia, an important physicochemical property, has a vital influence on the cell behaviors.

  7. Attachment and growth behaviour of human gingival fibroblasts on titanium and zirconia ceramic surfaces

    Energy Technology Data Exchange (ETDEWEB)

    Pae, Ahran; Kim, Hyeong-Seob; Woo, Yi-Hyung [Department of Prosthodontics, School of Dentistry, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701 (Korea, Republic of); Lee, Heesu [Department of Oral Anatomy, School of Dentistry, Kangnung National University, Gibyun-dong, Kangnung 210-702 (Korea, Republic of); Kwon, Yong-Dae, E-mail: ahranp@hotmail.co, E-mail: nightsu@kangnung.ac.k, E-mail: odontopia@khu.ac.k, E-mail: yongdae.kwon@gmail.co, E-mail: yhwoo@khu.ac.k [Department of Oral and Maxillofacial Surgery, School of Dentistry, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701 (Korea, Republic of)

    2009-04-15

    The attachment, growth behaviour and the genetic effect of human gingival fibroblasts (HGF) cultured on titanium and different zirconia surfaces were investigated. HGF cells were cultured on (1) titanium discs with a machined surface, (2) yttrium-stabilized tetragonal zirconia polycrystals (Y-TZP) with a smooth surface and (3) Y-TZP with 100{mu}m grooves. The cell proliferation activity was evaluated through a MTT assay at 24 h and 48 h, and the cell morphology was examined by SEM. The mRNA expression of integrin-beta1, type I and III collagen, laminin and fibronectin in HGF were evaluated by RT-PCR after 24 h. From the MTT assay, the mean optical density values for the titanium and grooved zirconia surfaces after 48 h of HGF adhesion were greater than the values obtained for the smooth zirconia surfaces. SEM images showed that more cells were attached to the grooves, and the cells appeared to follow the direction of the grooves. The results of RT-PCR suggest that all groups showed comparable fibroblast-specific gene expression. A zirconia ceramic surface with grooves showed biological responses that were comparable to those obtained with HGF on a titanium surface.

  8. Surface hydride on titanium by cathodic polarization promotes human gingival fibroblast growth.

    Science.gov (United States)

    Xing, Rui; Salou, Laëtitia; Taxt-Lamolle, Sébastien; Reseland, Janne E; Lyngstadaas, Ståle P; Haugen, Håvard J

    2014-05-01

    Connective tissue seal to dental abutment is crucial for peri-implant health. Several efforts have been made previously to optimize abutment surfaces, but no consensus has been reached regarding the optimal surface architecture and/or composition for soft tissue seal. Here, we report on experiments using cathodic polarization in organic acids to optimize titanium (Ti) surfaces for use as abutments. The three main factors affecting surface topography and chemistry were electrolyte composition, current density, and polarization time. Under identical conditions, oxalic acid created rougher surfaces than tartaric acid and acetic acid, and acetic acid produced more surface hydride. Surface hydride amount was suggested to first increase and then decrease with current density from 1 mA/cm(2) to 15 mA/cm(2) . The complexity of the surface topography and hydride production both increased with polarization time. Proliferation rate of human gingival fibroblasts (HGFs) was positively correlated with surface hydride content, suggesting the positive effect of surface hydride on connective tissue growth around dental abutment. Changes in surface topography and hydrophilicity did not significantly influence HGF growth.

  9. Anti-inflammatory activity of fisetin in human gingival fibroblasts treated with lipopolysaccharide.

    Science.gov (United States)

    Gutiérrez-Venegas, Gloria; Contreras-Sánchez, Anabel; Ventura-Arroyo, Jairo Agustín

    2014-10-01

    Fisetin is an anti-inflammatory flavonoid; however, its anti-inflammatory mechanism is not yet understood. In this study, we evaluated the anti-inflammatory effect of fisetin and its association with mitogen-activated protein kinase (MAPK) and nuclear factor kappa-beta pathways in human gingival fibroblasts (HGFs) treated with lipopolysaccharide (LPS) obtained from Porphyromonas gingivalis. The cell signaling, cell viability, and cyclooxygenase-2 (COX-2) expression of HGFs treated with various concentrations (0, 1, 5, 10, and 15 μM) of fisetin were measured by cell viability assay (MTT), Western blotting, and reverse transcriptase polymerase chain reaction analysis on COX-2. We found that fisetin significantly reduced the synthesis and expression of prostaglandin E2 in HGFs treated with LPS. Activation of extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38 MAPK was suppressed consistently by fisetin in HGFs treated with LPS. The data indicate that fisetin inhibits MAPK activation and COX-2 expression without affecting cell viability. These findings may be valuable for understanding the mechanism of the effect of fisetin on periodontal disease.

  10. Arecoline stimulated Cyr61 production in human gingival epithelial cells: inhibition by lovastatin.

    Science.gov (United States)

    Deng, Yi-Ting; Chang, Jenny Zwei-Chieng; Yeh, Cheng-Chang; Cheng, Shih-Jung; Kuo, Mark Yen-Ping

    2011-04-01

    Cyr61 is associated with growth and progression of many types of tumors and is an independent poor prognostic indicator for oral cancer patients. Areca nut (AN) chewing is the most important etiological factor in the pathogenesis of oral cancer in India and many Southeast Asian countries. Yet, the molecular mechanisms involved in the AN-induced oral cancer remain largely unknown. In this study, we show that arecoline, a main alkaloid found in AN, stimulated Cyr61 synthesis in human gingival epithelial S-G cells. Constitutive overexpression of Cyr61 protein in oral epithelial cells during AN chewing may play a role in the pathogenesis of oral cancer. ERK inhibitor PD98059, N-acetyl-L-cysteine, Rho-associated protein kinase (ROCK) selective inhibitor Y-27632 and a geranylgeranyltransferase inhibitor reduced the arecoline-stimulated levels of Cyr61 protein by ∼31%, 47%, 65% and 100%, respectively. Lovastatin also completely inhibited arecoline-induced Cyr61 synthesis and the inhibition is dose-dependent. Decreased of geranylgeranylated proteins could be the mechanism that lovastatin regulates Cyr61 synthesis and lovastatin could serve as a useful agent in controlling AN-induced oral cancer. Copyright © 2011 Elsevier Ltd. All rights reserved.

  11. Cytotoxicity of two available mineral trioxide aggregate cements and a new formulation on human gingival fibroblasts

    Science.gov (United States)

    Torshabi, Maryam; Amid, Reza; Kadkhodazadeh, Mahdi; Shahrbabaki, Sara Eslami; Tabatabaei, Fahimeh S.

    2016-01-01

    Aim: The purpose of this study was to investigate the cytotoxicity of nanohybrid mineral trioxide aggregate (MTA) in comparison with calcium-enriched mixture (CEM) cement and MTA-Angelus, using human gingival fibroblasts (HGFs). Materials and Methods: Nine disc-shaped specimens of each material (in 2 set stat: A, set for 24 h; B, set for 30 min; and C, fresh stat) were prepared. HGFs were exposed to tested materials’ extracts or control media. Cytotoxicity testing was performed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay in two time intervals. Statistical Analysis: Results were evaluated by one-way ANOVA and t-test. Statistical significance was set at P < 0.05. Results: CEM cement demonstrated favorable cell viability values when completely set (24 h set MTA = 24 h set CEM) at both time intervals. Interestingly, 24 h after incubation, CEM in Groups B and C demonstrated higher cell viability values than MTA (P < 0.05). However, after 72 h of incubation, these groups of CEM and MTA showed equal cell viability. All samples of nanohybrid MTA had slight cytotoxic effects after 24 h of incubation, and moderate cytotoxic effects after 72 h of incubation. Conclusion: Set CEM and set MTA-Angelus exerted similar, favorable effects on cell viability. However, within the limitations of this in vitro study, the results suggest that nanohybrid MTA could not be recommended as a material of choice for cervical root resorption. PMID:27994312

  12. Effects of titania nanotubes with or without bovine serum albumin loaded on human gingival fibroblasts.

    Science.gov (United States)

    Liu, Xiangning; Zhou, Xiaosong; Li, Shaobing; Lai, Renfa; Zhou, Zhiying; Zhang, Ye; Zhou, Lei

    2014-01-01

    Modifying the surface of the transmucosal area is a key research area because this process positively affects the three functions of implants: attachment to soft tissue, inhibiting bacterial biofilm adhesion, and the preservation of the crestal bone. To exploit the potential of titania nanotube arrays (TNTs) with or without using bovine serum albumin (BSA) to modify the surface of a dental implant in contact with the transmucosal area, BSA was loaded into TNTs that were fabricated by anodizing Ti sheets; the physical characteristics of these arrays, including their morphology, chemical composition, surface roughness, contact angle, and surface free energy (SFE), were assessed. The effect of Ti surfaces with TNTs or TNTs-BSA on human gingival fibroblasts (HGFs) was determined by analyzing cell morphology, early adhesion, proliferation, type I collagen (COL-1) gene expression, and the extracellular secretion of COL-1. The results indicate that early HGF adhesion and spreading behavior is positively correlated with surface characteristics, including hydrophilicity, SFE, and surface roughness. Additionally, TNT surfaces not only promoted early HGF adhesion, but also promoted COL-1 secretion. BSA-loaded TNT surfaces promoted early HGF adhesion, while suppressing late proliferation and COL-1 secretion. Therefore, TNT-modified smooth surfaces are expected to be applicable for uses involving the transmucosal area. Further study is required to determine whether BSA-loaded TNT surfaces actually affect closed loop formation of connective tissue because BSA coating actions in vivo are very rapid.

  13. IL-2,IL-17,IL-35 Expression in Peri-implant Gingival Crevicular Fluid and the Clinical Significance%种植体周围炎龈沟液中IL-2、IL-17、IL-35的表达及临床意义

    Institute of Scientific and Technical Information of China (English)

    尹光山; 王明德

    2015-01-01

    Objective To study interleukin(IL) 2,IL-17,IL-35 expression in peri-implantitis gingival crevicular fluid (GCF) and the clinical significance.Methods A total of 40 patients with implant surround-ing inflammation ( group A) from Linqu People′s Hospital were chosen as the research objects between Jan . 2011 and Dec.2013,while another 40 patients with healthy implant were chosen as group B,and 50 healthy people were chosen as healthy control group.The probing depth(PD),gingival sulcus bleeding index (SBI), the IL-2,IL-17,IL-35 mass concentration in the GCF of the three groups were recorded,and the correlation between IL-2,IL-17 in GCF,IL-35 mass concentration with gender,age,PD and SBI was analyzed.Results The PD,SBI,GCF of group A were significantly higher than group B and control group[(4.49 ±0.71) mm vs (2.59 ±0.62) mm,(2.54 ±0.59) mm;(3.45 ±0.89) vs (0.69 ±0.22),(0.64 ±0.27);(1.96 ± 0.82) μL vs (0.89 ±0.25) μL,(0.69 ±0.21) μL](P 0.05 ) .IL-7 of group A was higher than group B [(10.61 ±1.02) ng/L vs (7.58 ±0.98) ng/L,(5.49 ±0.91) ng/L](P <0.05),group B higher than the control group(P <0.05); IL-2,IL-35 level of group A were lower than group B and control group [(3.19 ±0.49) ng/L vs (3.69 ±0.68) ng/L,(3.91 ±0.72) ng/L;(62.78 ±4.29) ng/L vs (75.82 ± 4.63) ng/L,(82.49 ±4.81) ng/L](P<0.01),group B lower than the control group (P<0.05).IL-17 was positively correlated with age,PD and SBI(r=0.710,0.629,0.749,P <0.05),and IL-2,IL-35 were negatively correlated with the age, DP, SBI ( r =-0.698, -0.381, -0.761;r =-0.715, -0.387,-0.752, P<0.05).Conclusion IL-17 shows high expression in GCF in peri-implantitis,IL-2,IL-35 show low expression in GCF in peri-implantitis;the specific expressions of IL-35,IL-2,IL-17 in GCF are closely related to peri-implantitis.%目的:探讨白细胞介素(IL)2、IL-17、IL-35在种植体周围炎龈沟液中的表达及意义。方法选择2011年1月至2013年12月临朐县人民医院收治的40

  14. 母体唾液及龈沟液细胞因子水平与早产低体重新生儿关系的病例对照研究%Association between preterm low birth weight and maternal cytokine levels of saliva and gingival crevicular fluid

    Institute of Scientific and Technical Information of China (English)

    黄振; 沙月琴; 康军; 陈智滨; 苗江霞; 孙玉艳

    2010-01-01

    目的 验证牙周炎是否为早产低体重新生儿(preterm low birth weight,PLBW)母亲体内低度炎症的来源之一,初步探讨PLBW与炎症因子之间的关系.方法 以83例PLBW的母亲(PLBW组)及44名健康新生儿(normal birth weight,NBW)的母亲(NBW组)为研究对象,留取受试对象的静息全唾液、龈沟液,应用酶联免疫吸附试验检测唾液和龈沟液中白细胞介素(IL)-1β和IL-6水平,比较两组间唾液和龈沟液中细胞因子水平的差异,并分析细胞因子与分娩孕周、新生儿体重之间的相关性.结果 唾液IL-1β水平在PLBW组[(78.32±11.81)ng/L]显著高于NBW组[(39.66±11.89)ng/L],差异有统计学意义(P<0.05);分娩孕周与唾液IL-6之间呈显著的负相关关系(r=-0.274,P<0.01);新生儿体重与龈沟液IL-1β(r=-0.231,P<0.01)、唾液IL-6(r=-0.424,P<0.01)之间呈显著的负相关关系.结论 唾液和龈沟液中炎性因子水平越高,分娩孕周越短、新生儿体重越轻,母体牙周炎程度与PLBW之间可能存在一定程度的相关关系.%Objective To examine the hypothesis that periodontitis was one of the sources of inflammation in preterm low birth weight (PLBW) mothers and investigate the relationship between PLBW and inflammatory factors.Methods The samples of saliva and gingival crevicular fluid (GCF) were collected from 83 PLBW mothers(PLBW group) and 44 normal birth weight(NBW) mothers(NBW group).The levels of IL-1β and IL-6 were analyzed by enzyme-linked immunosorbent assay.The concentrations of cytokines in saliva and GCF were compared between the two groups.Results IL-1β level in saliva of PLBW group was higher than which of NBW group significantly[(78.32±11.81) ng/L vs.(39.66±11.89) ng/L, P<0.05].There was no statistically significant difference on IL-6 level in saliva[(17.45±3.13) ng/L vs.(16.28±0.66) ng/L], IL-1β level in GCF[(93.58±13.22) ng/L vs.(81.38±9.28) ng/L]and IL-6 level in GCF[(15.35±0.28) ng/L vs.(14.87 ±0.37) ng/L) between

  15. 牙周基础治疗对侵袭性牙周炎患者唾液和龈沟液中牙周致病菌的作用%Effect of non-surgical periodontal therapy on putative periodontal pathogens in saliva and gingival crevicular fluids in patients with aggressive periodontitis

    Institute of Scientific and Technical Information of China (English)

    路瑞芳; 冯向辉; 徐莉; 孟焕新

    2012-01-01

    Objective To analyze the effect of non-surgical periodontal treatment on the prevalence of putative periodontal pathogens in saliva and gingival crevicular fluids (GCF) in patients with aggressive periodontitis(AgP).Methods Sixteen patients with AgP received non-surgical periodontal treatment,and un-stimulated saliva and GCF were collected before treatment and 2 weeks,2 months,4 months,6 months post-treatment.Periodontal pathogens including Aggregatibacter actinomycetemcomitans (Aa),Porphyromonas gingivalis (Pg),Tannerella forsythia (Tf),Treponema denticola (Td),Prevotella intermedia (Pi) and Fusobacterium nucleatum (Fn) were detected by polymerase chain reaction.Results The Aa,Pg,Tf,Td,Pi and Fn in saliva were detected in 7,16,13,14,9 and 14 patients respectively at baseline,and in 3,9,5,4,4 and 7 patients 2 weeks post-treatment.The prevalence of the 6 periodontal pathogens showed a tendency to increase at 2 months post-treatment,and was almost the same as that of baseline at 6 months post-treatment.While in GCF the prevalence of Aa,Pg,Tf,Td,Pi and Fn were 17% (11/64),95% (61/64),84% (54/64),72% (46/64),41% (26/64) and 56% (36/64) at baseline,and reduced to 8% (5/64),75% (48/64),28% (18/64),14% (9/64),16% (10/64) and 23% (15/64) at 2 weeks post-treatment (P < 0.05).The prevalence of the 6 periodontal pathogens remained a relatively low level [13% (8/64),73% (47/64),42% (27/64),23% (15/64),8% (5/64) and 38% (24/64)respectively] at 6 months post-treatment in GCF(P < 0.05) compared with baseline except Aa.There was difference of periodontal pathogens detection between saliva and GCF.Conclusions Non-surgical periodontal treatment can reduce pocket depth as well as periodontal pathogens in saliva and GCF.Periodontal ruaintenance every 2 months can keep the periodontal pathogens in GCF at a relatively low level.The periodontal pathogen detection in saliva can not completely replace the site detection.%目的

  16. Oral hygiene status and expression of inflammatory factors in peripheral blood and gingival crevicular fluid of elderly pa-tients with coronary artery disease%老年冠心病患者口腔牙周现况及外周血、龈沟液炎性标识指标对照分析

    Institute of Scientific and Technical Information of China (English)

    张力; 朱浩

    2014-01-01

    目的:对照分析老年冠心病( CHD)患者口腔牙周现况及外周血、龈沟液( GCF)炎性标识指标水平。方法连续选择2013年1月到2013年4月在解放军沈阳军区总医院心内科住院治疗的老年CHD患者47例,对照组入选同时间参加我院体检结论健康老年人42例。两组对象均接受了口腔牙周检查及外周血、GCF处炎性标识指标( IL-1β、IL-6、TNF-α和hs-CRP)检测。结果老年CHD组的牙周炎及牙周病患病率分别为53.19%(25例)和72.34%(34例),均明显高于对照组(13例,27.65%,χ2=6.59和10例,23.81%,χ2=9.15;均P<0.01)。老年CHD组的临床附着丧失、牙周探诊深度、龈沟出血指数、牙齿松动度、牙齿填充指数及简化口腔卫生指数均明显差于对照组(P<0.05,P<0.01);老年CHD组的外周血IL-1β、IL-6、TNF-α和hs-CRP水平均显著高于对照组,老年CHD组的GCF处IL-1β、IL-6、TNF-α均显著高于对照组( P<0.05P<0.01)。结论老年CHD患者口腔牙周卫生状况差,牙周疾病患病率增加,外周血及GCF处炎性标识指标水平明显升高。%Objective To investigate the oral hygiene status and to analyze the expression of inflammatory factors in the periph -eral blood and gingival crevicular fluid ( GCF) of elderly patients with coronary artery disease ( CHD) .Methods A total of 47 CHD elderly patients and 42 healthy controls were selected from Department of Cardiology , General Hospital of Shenyang Military Area Command of Chinese PLA from January to Apirl ,2013.All the cases were treated by periodontal examination and measurements of in-flammatory factors,including IL-1β,IL-6,TNF-αand hs-CRP,in the peripheral blood and GCF.Results The periodontitis preva-lence was 53.19%(n=25),and the prevalence of periodontal disease was 72.34%(n=34) in the CHD group,which were signifi-cantly higher than 27.65%(n=13,x=6.59) and 23.81%(n=10,x=9.15) in the

  17. Effect of special intervention on expression of inflammatory factors in peripheral blood and gingival crevicular fluid of elderly patients with coronary heart disease and periodontitis%专项干预对老年冠心病合并牙周炎患者口腔、外周血及龈沟液炎性指标影响

    Institute of Scientific and Technical Information of China (English)

    张力; 朱浩

    2014-01-01

    目的:观察专项干预对老年冠心病(CHD)合并牙周炎患者口腔、外周血及龈沟液(GCF)炎性指标影响。方法连续选择近期在解放军沈阳军区总医院心内科住院治疗的老年CHD合并牙周炎患者45例。入选对象随机分为口腔干预组(23例)和对照组(22例)。两组患者均接受了常规冠心病治疗,口腔干预组另接受了口腔专项干预治疗。治疗前后进行了口腔、外周血及GCF处炎性指标检测。结果两组患者治疗前各类口腔及牙周相关指标接近,治疗后口腔干预组的PD、CAL、M、SBI及OHI-S均明显好于治疗前及对照组同期结果(P均<0.05);两组患者治疗前外周血炎性标识指标水平接近,两组患者治疗后外周血炎性标识指标水平均明显低于治疗前,同时,口腔干预组的IL-1β、IL-6、TNF-α和hs-CRP表达水平均显著低于对照组(P均<0.05);两组患者治疗前GCF处炎性标识指标水平接近,治疗后口腔干预组GCF处IL-1β、IL-6和TNF-α水平均显著低于治疗前及对照组同期结果(P均<0.05~0.01)。结论专项干预可明确改善老年冠心病(CHD)合并牙周炎患者口腔、外周血及龈沟液(GCF)炎性指标。%Objective To investigate the effect of special intervention on the expression of inflammatory factors in the oral cavity, peripheral blood and gingival crevicular fluid (GCF) of elderly patients with coronary heart disease (CHD) and periodontitis. Methods A total of 45 elderly patients with CHD and periodontitis were selected from Department of Cardiology, General Hospital of Shenyang Military Area Command of Chinese PLA and divided into intervention group (n=23) and control group (n=22). All the cases were treated by general therapy;meanwhile, the cases in the intervention group were also treated by special intervention. Inflammatory factors in the oral cavity, peripheral blood and GCF were tested before and after treatment. Results Related indicators of

  18. Significance of Circulating and Crevicular Matrix Metalloproteinase-9 in Rheumatoid Arthritis-Chronic Periodontitis Association

    Directory of Open Access Journals (Sweden)

    Isabela Silosi

    2015-01-01

    periodontal disease have been identified. Emerging as a chronic inflammatory joint disease, RA displays various features and pathogenetic events similar to chronic periodontitis (CP. The purpose of this study was to evaluate the utility of determining systemic and crevicular levels of metalloproteinase-9 (MMP-9 as potential biomarkers for association between RA and CP. A total of fifty-six patients were included in the study. The subjects were categorized into four groups as follows: healthy-control (n=21, active RA (n=16, CP (n=14, and RA-CP association (n=12. Assessment of serum and crevicular concentrations of total MMP-9 (active and pro-MMP-9 was based on ELISA technique. The results of this study showed statistically significant differences of serum MMP-9 between patients groups and control. Serum levels of MMP-9 were similar in RA and RA-CP associated patients. Gingival crevicular fluid (GCF recorded increased MMP-9 levels in RA-CP association subjects as compared to CP. Considering that RA-CP association is characterized by a disregulation of the inflammatory response, MMP-9 may play a role in the pathogenesis of RA-CP association. MMP-9 is therefore a sensitive tool in the diagnosis and management of patients affected by this binomial association.

  19. High-frequency low-level diode laser irradiation promotes proliferation and migration of primary cultured human gingival epithelial cells.

    Science.gov (United States)

    Ejiri, Kenichiro; Aoki, Akira; Yamaguchi, Yoko; Ohshima, Mitsuhiro; Izumi, Yuichi

    2014-07-01

    In periodontal therapy, the use of low-level diode lasers has recently been considered to improve wound healing of the gingival tissue. However, its effects on human gingival epithelial cells (HGECs) remain unknown. The aim of the present study was to examine whether high-frequency low-level diode laser irradiation stimulates key cell responses in wound healing, proliferation and migration, in primary cultured HGECs in vitro. HGECs were derived from seven independent gingival tissue specimens. Cultured HGECs were exposed to a single session of high-frequency (30 kHz) low-level diode laser irradiation with various irradiation time periods (fluence 5.7-56.7 J/cm(2)). After 20-24 h, cell proliferation was evaluated by WST-8 assay and [(3)H]thymidine incorporation assay, and cell migration was monitored by in vitro wound healing assay. Further, phosphorylation of the mitogen-activated protein kinase (MAPK) pathways after irradiation was investigated by Western blotting. The high-frequency low-level irradiation significantly increased cell proliferation and [(3)H]thymidine incorporation at various irradiation time periods. Migration of the irradiated cells was significantly accelerated compared with the nonirradiated control. Further, the low-level diode laser irradiation induced phosphorylation of MAPK/extracellular signal-regulated protein kinase (ERK) at 5, 15, 60, and 120 min after irradiation. Stress-activated protein kinases/c-Jun N-terminal kinase and p38 MAPK remained un-phosphorylated. The results show that high-frequency low-level diode laser irradiation promotes HGEC proliferation and migration in association with the activation of MAPK/ERK, suggesting that laser irradiation may accelerate gingival wound healing.

  20. Isolation and characterisation of human gingival margin-derived STRO-1/MACS1 and MACS2 cell populations

    Institute of Scientific and Technical Information of China (English)

    Karim M Fawzy El-Sayed; Sebastian Paris; Christian Graetz; Neemat Kassem; Mohamed Mekhemar; Hendrick Ungefroren; Fred Fandrich; Christof Dorfer

    2015-01-01

    Recently, gingival margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting (MACS) showed remarkable periodontal regenerative potential in vivo. As a second-stage investigation, the present study’s aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive (MACS1) and STRO-1-negative (MACS2) cell populations from the human free gingival margin. Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies. Subsequently, the MACS1 and MACS2 cell fractions were characterized by flow cytometry for expression of CD14, CD34, CD45, CD73, CD90, CD105, CD146/MUC18 and STRO-1. Colony-forming unit (CFU) and multilineage differentiation potential were assayed for both cell fractions. Mineralisation marker expression was examined using real-time polymerase chain reaction (PCR). MACS1 and MACS2 cell fractions showed plastic adherence. MACS1 cells, in contrast to MACS2 cells, showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs (P,0.01). More than 95%of MACS1 cells expressed CD105, CD90 and CD73;lacked the haematopoietic markers CD45, CD34 and CD14, and expressed STRO-1 and CD146/MUC18. MACS2 cells showed a different surface marker expression profile, with almost no expression of CD14 or STRO-1, and more than 95%of these cells expressed CD73, CD90 and CD146/MUC18, as well as the haematopoietic markers CD34 and CD45 and CD105. MACS1 cells could be differentiated along osteoblastic, adipocytic and chondroblastic lineages. In contrast, MACS2 cells demonstrated slight osteogenic potential. Unstimulated MACS1 cells showed significantly higher expression of collagen I (P,0.05) and collagen III (P,0.01), whereas MACS2 cells demonstrated higher expression of osteonectin (P,0.05;Mann–Whitney). The present study is the first to compare gingival

  1. Butyrate induces reactive oxygen species production and affects cell cycle progression in human gingival fibroblasts.

    Science.gov (United States)

    Chang, M-C; Tsai, Y-L; Chen, Y-W; Chan, C-P; Huang, C-F; Lan, W-C; Lin, C-C; Lan, W-H; Jeng, J-H

    2013-02-01

    Short-chain fatty acids, such as butyric acid and propionic acid, are metabolic by-products generated by periodontal microflora such as Porphyromonas gingivalis, and contribute to the pathogenesis of periodontitis. However, the effects of butyrate on the biological activities of gingival fibroblasts (GFs) are not well elucidated. Human GFs were exposed to various concentrations of butyrate (0.5-16 mm) for 24 h. Viable cells that excluded trypan blue were counted. Cell cycle distribution of GFs was analyzed by propidium iodide-staining flow cytometry. Cellular reactive oxygen species (ROS) production was measured by flow cytometry using 2',7'-dichlorofluorescein (DCF). Total RNA and protein lysates were isolated and subjected to RT-PCR using specific primers or to western blotting using specific antibodies, respectively. Butyrate inhibited the growth of GFs, as indicated by a decrease in the number of viable cells. This event was associated with an induction of G0/G1 and G2/M cell cycle arrest by butyrate (4-16 mm) in GFs. However, no marked apoptosis of GFs was noted in this experimental condition. Butyrate (> 2 mm) inhibited the expression of cdc2, cdc25C and cyclinB1 mRNAs and reduced the levels of Cdc2, Cdc25C and cyclinB1 proteins in GFs, as determined using RT-PCR and western blotting, respectively. This toxic effect of butyrate was associated with the production of ROS. These results suggest that butyrate generated by periodontal pathogens may be involved in the pathogenesis of periodontal diseases via the induction of ROS production and the impairment of cell growth, cell cycle progression and expression of cell cycle-related genes in GFs. These events are important in the initiation and prolongation of inflammatory processes in periodontal diseases. © 2012 John Wiley & Sons A/S.

  2. The capsule of Porphyromonas gingivalis reduces the immune response of human gingival fibroblasts

    Directory of Open Access Journals (Sweden)

    van Winkelhoff Arie J

    2010-01-01

    Full Text Available Abstract Background Periodontitis is a bacterial infection of the periodontal tissues. The Gram-negative anaerobic bacterium Porphyromonas gingivalis is considered a major causative agent. One of the virulence factors of P. gingivalis is capsular polysaccharide (CPS. Non-encapsulated strains have been shown to be less virulent in mouse models than encapsulated strains. Results To examine the role of the CPS in host-pathogen interactions we constructed an insertional isogenic P. gingivalis knockout in the epimerase-coding gene epsC that is located at the end of the CPS biosynthesis locus. This mutant was subsequently shown to be non-encapsulated. K1 capsule biosynthesis could be restored by in trans expression of an intact epsC gene. We used the epsC mutant, the W83 wild type strain and the complemented mutant to challenge human gingival fibroblasts to examine the immune response by quantification of IL-1β, IL-6 and IL-8 transcription levels. For each of the cytokines significantly higher expression levels were found when fibroblasts were challenged with the epsC mutant compared to those challenged with the W83 wild type, ranging from two times higher for IL-1β to five times higher for IL-8. Conclusions These experiments provide the first evidence that P. gingivalis CPS acts as an interface between the pathogen and the host that may reduce the host's pro-inflammatory immune response. The higher virulence of encapsulated strains may be caused by this phenomenon which enables the bacteria to evade the immune system.

  3. Identification of an anti-inflammatory potential of Eriodictyon angustifolium compounds in human gingival fibroblasts.

    Science.gov (United States)

    Walker, Jessica; Reichelt, Katharina V; Obst, Katja; Widder, Sabine; Hans, Joachim; Krammer, Gerhard E; Ley, Jakob P; Somoza, Veronika

    2016-07-13

    Polyphenol-rich plant extracts have been shown to possess anti-inflammatory activity against oral pathogen-induced cytokine release in model systems of inflammation. Here, it was hypothesized that a flavanone-rich extract of E. angustifolium exhibits an anti-inflammatory potential against endotoxin-induced inflammatory response in human gingival fibroblasts (HGF-1). HGF-1 cells were stimulated with lipopolysaccharide from Porphyromonas gingivalis (pg-LPS) to release pro-inflammatory cytokines. Concentrations of interleukins IL-6 and IL-8 and macrophage chemoattractant protein-1 in the incubation media upon stimulation were determined by means of magnetic bead analysis. A crude ethanol/water extract of E. angustifolium (EE) was fractionated via gel permeation chromatography into a flavanone-rich fraction (FF) and an erionic acid-rich fraction (EF). Individual flavanones and erionic acids as well as EE, EF and FF were tested in the pg-LPS-stimulated HGF-1 cells for their anti-inflammatory potential. The E. angustifolium extract possessed anti-inflammatory potential in this model system, attenuating the pg-LPS-induced release of IL-6 by up to 52.0 ± 15.5%. Of the individual flavanones, eriodictyol and naringenin had the most pronounced effect. However, a mixture of the flavanones did not possess the same effect as the entire flavanoid fraction, indicating that other compounds may contribute to the anti-inflammatory potential of E. angustifolium. For the first time, an anti-inflammatory potential of E. angustifolium and containing erionic acids has been determined.

  4. The effect of systemic administration of ibuprofen in the experimental gingivitis model.

    Science.gov (United States)

    Sekino, Satoshi; Ramberg, Per; Lindhe, Jan

    2005-02-01

    Studies in humans have indicated that systemically administered flurbiprofen and ibuprofen may reduce gingivitis. De novo plaque formation is enhanced at tooth surfaces adjacent to inflamed gingivae. The aim of the present clinical trial was to evaluate the effect of systemic administration of ibuprofen on gingivitis and plaque build-up. Eleven subjects were recruited for the study and were given oral hygiene instruction, scaling and professional mechanical tooth cleaning (PTC). At the end of a preparatory period (Day 0), the participants were told to abstain from all mechanical plaque control measures during a 2-week experimental period but to rinse with an assigned mouth rinse (positive control: 0.1% chlorhexidine digluconate; negative control: saline) or administer ibuprofen (tablets of 200 mg twice daily). Mouth rinsing was performed twice a day (after breakfast and in the evening), for 60 s with 10 ml. Re-examination was performed after 14 days of experiment. After a 2-week "wash-out" period, the participants received a new PTC and a second 14-day experimental period was initiated. The experimental and "wash-out" periods were repeated until all volunteers had been involved in all three regimens. Dental plaque was scored using the Quigley & Hein Plaque Index system and gingivitis according to the Gingival Index (GI) system. Supragingival plaque was collected and prepared for dark-field microscopy. One hundred bacterial cells were counted and classified into six different groups: coccoid cells, straight rods, filaments, fusiforms, spirochetes and motile rods. Gingival crevicular fluid (GCF) was collected from the same sites that were sampled for plaque. The volume of GCF collected in each strip was measured and analysed regarding content of lactoferrin and albumin. During the period when the panelists rinsed with saline they accumulated large amounts of plaque and developed marked signs of gingivitis. When they rinsed with chlorhexidine digluconate, small

  5. Cell death effects of resin-based dental material compounds and mercurials in human gingival fibroblasts

    Energy Technology Data Exchange (ETDEWEB)

    Reichl, Franz-Xaver [Walther-Straub-Institute of Pharmacology and Toxicology, Munich (Germany); Ludwig-Maximilians-University, Department of Operative Dentistry and Periodontology, Munich (Germany); Esters, Magali; Simon, Sabine; Seiss, Mario [Walther-Straub-Institute of Pharmacology and Toxicology, Munich (Germany); Kehe, Kai [Bundeswehr Institute of Pharmacology and Toxicology, Munich (Germany); Kleinsasser, Norbert [University of Regensburg, Head and Neck Surgery, Department of Otolaryngology, Regensburg (Germany); Folwaczny, Matthias; Glas, Juergen; Hickel, Reinhard [Ludwig-Maximilians-University, Department of Operative Dentistry and Periodontology, Munich (Germany)

    2006-06-15

    In order to test the hypothesis that released dental restorative materials can reach toxic levels in human oral tissues, the cytotoxicities of the resin-based dental (co)monomers hydroxyethylmethacrylate (HEMA), triethyleneglycoldimethacrylate (TEGDMA), urethanedimethacrylate (UDMA), and bisglycidylmethacrylate (BisGMA) compared with methyl mercury chloride (MeHgCl) and the amalgam component mercuric chloride (HgCl{sub 2}) were investigated on human gingival fibroblasts (HGF) using two different test systems: (1) the modified XTT-test and (2) the modified H 33342 staining assay. The HGF were exposed to various concentrations of the test-substances in all test systems for 24 h. All tested (co)monomers and mercury compounds significantly (P<0.05) decreased the formazan formation in the XTT-test. EC{sub 50} values in the XTT assay were obtained as half-maximum-effect concentrations from fitted curves. Following EC{sub 50} values were found (mean [mmol/l]; s.e.m. in parentheses; n=12; * significantly different to HEMA): HEMA 11.530 (0.600); TEGDMA* 3.460 (0.200); UDMA* 0.106 (0.005); BisGMA* 0.087 (0.001); HgCl{sub 2}* 0.013 (0.001); MeHgCl* 0.005 (0.001). Following relative toxicities were found: HEMA 1; TEGDMA 3; UDMA 109; BisGMA 133; HgCl{sub 2} 887; MeHgCl 2306. A significant (P<0.05) increase of the toxicity of (co)monomers and mercurials was found in the XTT-test in the following order: HEMA < TEGDMA < UDMA < BisGMA < HgCl{sub 2} < MeHgCl. TEGDMA and MeHgCl induced mainly apoptotic cell death. HEMA, UDMA, BisGMA, and HgCl{sub 2} induced mainly necrotic cell death. The results of this study indicate that resin composite components have a lower toxicity than mercury from amalgam in HGF. HEMA, BisGMA, UDMA, and HgCl{sub 2} induced mainly necrosis, but it is rather unlikely that eluted substances (solely) can reach concentrations, which might induce necrotic cell death in the human physiological situation, indicating that other (additional) factors may be involved in

  6. 侵袭性牙周炎龈沟液中有机酸与牙龈卟啉单胞菌和齿垢密螺旋体的关系%Relationship between volatile fatty acids and Porphyromonas gingivalis and Treponema denticola in gingival crevicular fluids of patients with aggressive periodontitis

    Institute of Scientific and Technical Information of China (English)

    路瑞芳; 冯琳; 高学军; 孟焕新; 冯向辉

    2013-01-01

    Short chain fatty acids (SCFAs), such as succinic acid, acetic acid, propionic acid, butyric acid, etc. are metabolic product of putative periodontal pathogens, which play significant roles in periodontitis. The aim of this study was to analyze the relationship between Porphyromonas gingivalis (P. gingivalis) , Treponema denticola (T. denticola) , and the concentration of SCFAs in gingival crevicular fluid ( GCF) of patients with aggressive periodontitis ( AgP). Methods; GCF was sampled from 4 sites per individual in 20 patients with AgP and 14 healthy controls. Concentrations of SCFAs, including succinic acid, acetic acid, propionic acid, butyric acid, and isovaleric acid in the supernant of GCF were analyzed by high performance capillary electrophoresis (HPCE), P. gingivalis and T. denticola in the deposit of the same GCF were detected by PCR with their electrophoretic band quantified. Results : The concentrations of succinic acid, acetic acid, propionic acid, butyric acid, and isovaleric acid, the prevalence and PCR band quantity of P. gingivalis and T. denticola in GCF were all significantly higher in patients with AgP than that of healthy controls. In patients with AgP, butyric acid concentration was significantly higher in P. gingivalis positive sites than negative sites [2.87 (0.99, 4. 36) mmol/L vs. 0.33 (0.00, 1.44) mmol/L, P<0.05], the concentrations of succinic acid, acetic acid, propionic acid, butyric acid, and isovaleric acid were positively correlated with PCR band quantity of P. gingivalis (r value was 0. 334, 0. 548, 0.411, 0. 493, 0. 273, respectively, P < 0. 05); the concentrations of SCFAs were significantly higher in T. denticola positive sites than negative sites; succinic acid, 1. 67 (1.15, 2.11) mmol/L m. 0.80 (0.48, 1.06) mmol/L; acetic acid, 31.95 (23.77, 43.13) mmol/L vs. 12.51 (7.57, 15.69) mmol/L; propionic acid, 11.86 (6.55, 14.98) mmol/L vs. 2.82 (1.71, 7.03) mmol/L; butyric acid, 3.45 (2.41,4.78) mmol/L vs. 0.54 (0.00, 1.56) mmol

  7. The phylum Synergistetes in gingivitis and necrotizing ulcerative gingivitis.

    Science.gov (United States)

    Baumgartner, Angelica; Thurnheer, Thomas; Lüthi-Schaller, Helga; Gmür, Rudolf; Belibasakis, Georgios N

    2012-11-01

    The clinical manifestation of necrotizing ulcerative gingivitis (NUG) is distinct from that of common gingivitis in that it is characterized by local necrosis of the gingival tissues, rapid onset, pain and extensive bleeding. The phylum Synergistetes is a novel bacterial phylum consisting of Gram-negative anaerobes, with evidence of presence in biofilms associated with periodontal and endodontic infections. To date, the involvement of members of this phylum in NUG has not been investigated. This study aimed to evaluate the presence and levels of known human oral Synergistetes bacterial clusters in dental plaque from patients with NUG and compare them with those found in gingivitis. Marginal dental plaque samples from 21 NUG and 21 gingivitis patients were analysed quantitatively by fluorescent in situ hybridization and microscopy for members of two oral Synergistetes clusters (A and B) and for Jonquetella anthropi. Synergistetes cluster A bacteria were detected in all samples but at higher levels (9.4-fold) and proportions (2.5-fold) in NUG patients than in gingivitis patients. However, with regard to Synergistetes cluster B bacteria, there were no differences between NUG and gingivitis patients. J. anthropi was detected in only half of the samples and at lower levels than the other taxa. In conclusion, these data demonstrate that Synergistetes cluster A bacteria, but not cluster B bacteria or J. anthropi, are more strongly associated with NUG than with gingivitis.

  8. The influence of SrO and CaO in silicate and phosphate bioactive glasses on human gingival fibroblasts.

    Science.gov (United States)

    Massera, J; Kokkari, A; Närhi, T; Hupa, L

    2015-06-01

    In this paper, we investigate the effect of substituting SrO for CaO in silicate and phosphate bioactive glasses on the human gingival fibroblast activity. In both materials the presence of SrO led to the formation of a CaP layer with partial Sr substitution for Ca. The layer at the surface of the silicate glass consisted of HAP whereas at the phosphate glasses it was close to the DCPD composition. In silicate glasses, SrO gave a faster initial dissolution and a thinner reaction layer probably allowing for a continuous ion release into the solution. In phosphate glasses, SrO decreased the dissolution process and gave a more strongly bonded reaction layer. Overall, the SrO-containing silicate glass led to a slight enhancement in the activity of the gingival fibroblasts cells when compared to the SrO-free reference glass, S53P4. The cell activity decreased up to 3 days of culturing for all phosphate glasses containing SrO. Whereas culturing together with the SrO-free phosphate glass led to complete cell death at 7 days. The glasses containing SrO showed rapid cell proliferation and growth between 7 and 14 days, reaching similar activity than glass S53P4. The addition of SrO in both silicate and phosphate glasses was assumed beneficial for proliferation and growth of human gingival fibroblasts due to Sr incorporation in the reaction layer at the glass surface and released in the cell culture medium.

  9. Human dental pulp stem cells and gingival fibroblasts seeded into silk fibroin scaffolds have the same ability in attracting vessels

    Directory of Open Access Journals (Sweden)

    Anna eWoloszyk

    2016-04-01

    Full Text Available Neovascularization is one of the most important processes during tissue repair and regeneration. Current healing approaches based on the use of biomaterials combined with stem cells in critical-size bone defects fail due to the insufficient implant vascularization and integration into the host tissues. Therefore, here we studied the attraction, ingrowth, and distribution of blood vessels from the chicken embryo chorioallantoic membrane into implanted silk fibroin scaffolds seeded with either human dental pulp stem cells or human gingival fibroblasts. Perfusion capacity was evaluated by non-invasive in vivo Magnetic Resonance Imaging while the number and density of blood vessels were measured by histomorphometry. Our results demonstrate that human dental pulp stem cells and gingival fibroblasts possess equal abilities in attracting vessels within silk fibroin scaffolds. Additionally, the prolonged in vitro pre-incubation period of these two cell populations favors the homogeneous distribution of vessels within silk fibroin scaffolds, which further improves implant survival and guarantees successful healing and regeneration.

  10. ETM study of electroporation influence on cell morphology in human malignant melanoma and human primary gingival fibroblast cells

    Institute of Scientific and Technical Information of China (English)

    Nina Skolucka; Malgorzata Daczewska; Jolanta Saczko; Agnieszka Chwilkowska; Anna Choromanska; Malgorzata Kotulska; Iwona Kaminska; Julita Kulbacka

    2011-01-01

    Objective:To estimate electroporation (EP) influence on malignant and normal cells.Methods:Two cell lines including human malignant melanoma (Me-45) and normal human gingival fibroblast (HGFs) were used. EP parameters were the following:250,1000,1750,2500 V/cm;50 μs by5 impulses for every case. The viability of cells after EP was estimated byMTT assay. The ultrastructural analysis was observed by transmission electron microscope (ZeissEM900). Results:In the current study we observed the intracellular effect followingEP on Me-45 and HGF cells. At the conditions applied, we did not observe any significant damage of mitochondrial activity in both cell lines treated byEP. Conversely, we showed thatEP in some conditions can stimulate cells to proliferation. Some changes induced byEP were only visible in electron microscopy. In fibroblast cells we observed significant changes in lower parameters ofEP (250 and1000 V/cm). After applying higher electric field intensities (2500 V/cm) we detected many vacuoles, myelin-like bodies and swallowed endoplasmic reticulum. In melanoma cells such strong pathological modifications afterEP were not observed, in comparison with control cells. The ultrastructure of both treated cell lines was changed according to the applied parameters ofEP.Conclusions:We can claim thatEP conditions are cell line dependent. In terms of the intracellular morphology, human fibroblasts are more sensitive to electric field as compared with melanoma cells. Optimal conditions should be determined for each cell line. Summarizing our study, we can conclude thatEP is not an invasive method for human normal and malignant cells. This technique can be safely applied in chemotherapy for delivering drugs into tumor cells.

  11. ETM study of electroporation influence on cell morphology in human malignant melanoma and human primary gingival fibroblast cells.

    Science.gov (United States)

    Skolucka, Nina; Daczewska, Malgorzata; Saczko, Jolanta; Chwilkowska, Agnieszka; Choromanska, Anna; Kotulska, Malgorzata; Kaminska, Iwona; Kulbacka, Julita

    2011-04-01

    To estimate electroporation (EP) influence on malignant and normal cells. Two cell lines including human malignant melanoma (Me-45) and normal human gingival fibroblast (HGFs) were used. EP parameters were the following: 250, 1 000, 1 750, 2 500 V/cm; 50 µs by 5 impulses for every case. The viability of cells after EP was estimated by MTT assay. The ultrastructural analysis was observed by transmission electron microscope (Zeiss EM 900). In the current study we observed the intracellular effect following EP on Me-45 and HGF cells. At the conditions applied, we did not observe any significant damage of mitochondrial activity in both cell lines treated by EP. Conversely, we showed that EP in some conditions can stimulate cells to proliferation. Some changes induced by EP were only visible in electron microscopy. In fibroblast cells we observed significant changes in lower parameters of EP (250 and 1 000 V/cm). After applying higher electric field intensities (2 500 V/cm) we detected many vacuoles, myelin-like bodies and swallowed endoplasmic reticulum. In melanoma cells such strong pathological modifications after EP were not observed, in comparison with control cells. The ultrastructure of both treated cell lines was changed according to the applied parameters of EP. We can claim that EP conditions are cell line dependent. In terms of the intracellular morphology, human fibroblasts are more sensitive to electric field as compared with melanoma cells. Optimal conditions should be determined for each cell line. Summarizing our study, we can conclude that EP is not an invasive method for human normal and malignant cells. This technique can be safely applied in chemotherapy for delivering drugs into tumor cells.

  12. Effects of resolvin D1 on cell survival and cytokine expression of human gingival fibroblasts.

    Science.gov (United States)

    Khaled, Mohamed; Shibani, Nouf-Al; Labban, Nawaf; Batarseh, Ghada; Song, Fengyu; Ruby, John; Windsor, L Jack

    2013-12-01

    Tissue breakdown in periodontitis is initiated by bacteria, such as Porphyromonas gingivalis, and is caused largely by host responses. Resolvins protect the host against acute inflammation by blocking the migration of polymorphonuclear neutrophils to initiate resolution. The effects of resolvins on human gingival fibroblasts (HGFs) are unknown. This study examines the effects of resolvin D1 on HGF survival and cytokine expression when treated with or without P. gingivalis supernatant. Cytotoxicity of resolvin D1 on HGFs with or without a toxic level of P. gingivalis supernatant was measured with lactate dehydrogenase assays. Cytokine arrays were performed on HGF-conditioned media treated with or without resolvin D1 and with or without P. gingivalis supernatant. Resolvin D1 had no cytotoxic effects on HGFs at concentrations between 1 and 1,000 nM (all P > 0.05). Resolvin D1 (1,000 nM) significantly inhibited the toxic effects of 13.5% (v/v) P. gingivalis supernatant on HGFs (P = 0.002). Resolvin D1 significantly reduced the expression of interleukin (IL)-6 (P = 0.010) and monocyte chemoattractant protein (MCP)-1 (P = 0.04) in untreated fibroblasts. P. gingivalis (10%) supernatant significantly increased the expression levels of granulocyte-macrophage colony-stimulating factor (CSF), granulocyte CSF, growth-regulated oncogene (GRO), IL-5, IL-6, IL-7, IL-8, IL-10, MCP-1, MCP-2, MCP-3, and monokine induced by γ-interferon. Resolvin D1 significantly reduced the expression of GRO (P = 0.04), marginally reduced the levels of MCP-1 (P = 0.10), and marginally increased the levels of transforming growth factor (TGF)-β1 (P = 0.07) from HGFs treated with P. gingivalis supernatant. Resolvin D1 altered the cytotoxicity of P. gingivalis supernatant on HGFs. Resolvin D1 significantly reduced GRO, marginally reduced MCP-1, and marginally increased TGF-β1 from P. gingivalis-treated HGFs, which could alter the ability of P. gingivalis to induce inflammation.

  13. Impact of Dilution and Polymerization on Cytotoxicity of Dentin Adhesives to Human Gingival Fibroblasts: Early Exposure Time.

    Science.gov (United States)

    Banava, Sepideh; Najibfard, Kaveh; Garcia-Godoy, Franklin; Saghiri, Mohammad Ali; Ghahremani, Mohammad Hossein; Ostad, Naser

    2015-01-01

    Background and aims. The aim of this study was to evaluate the effect of dilution and curing methods of an etch-and-rinse adhesive and a self-etching primer from the same manufacturer at early exposure time on cytotoxicity of primary human gingival fibroblasts. Materials and methods. Primary human gingival fibroblasts were exposed to different dilutions of Adper Single Bond (ASB) and Adper Prompt L-Pop (APL) (3M ESPE, USA). They were evaluated in unpolymerized mode for 20 s, 5 min and 24 h and in polymerized mode for 24 h and 48 h. Cytotoxicity was evaluated using three cytotoxic tests (MTT, cell counting and DNA condensation). Data was analyzed by a one-way ANOVA and Post Hoc Tukey HSD test. Results. Cytotoxicity tests revealed that unpolymerized APL was more cytotoxic compared to ASB after 20 s (Pcytotoxic than APL with lower dilutions. Polymerized ASB was more toxic than APL. Conclusion. Both adhesives were cytotoxic in different dilutions, times and curing modes. Cytotoxicity of the unpolymerized self-etching primer (APL) was more than etch-and-rinse adhesive (ASB) in 20 s, which is important clinically and dentists should be aware of the harmful effects and try to minimize it by curing and rinsing soon after composite resin insertion. ASB was more cytotoxic at 5 min and 24h.

  14. Morphological evaluations of human gingival fibroblasts exposed to novel dental adhesives in combination with glucocorticoids or dihydropridines - biomed 2013.

    Science.gov (United States)

    Garner, Angelia D; Tucci, Michelle A; Benghuzzi, Hamed A

    2013-01-01

    The present study evaluated the morphology of human gingival fibroblasts when exposed to dental adhesives in combination with glucocorticoids or dihydropridines in an in vitro environment. The cultured gingival fibroblasts used were obtained from the American Type Culture Collection (ATCC). The three dental adhesives used were Polymethyl methacrylate, OptiBond®, and Prime & Bond®. The glucocorticoid of choice was Cortisol along with the dihydropridine (Nifedipine). Fibroblasts were treated with each bonding agent, Cortisol, or Nifedipine for durations of 24, 48, and 72 hours. Morphological evaluation revealed that at 24 and 48 hour phases, there were no observable differences between the control and groups exposed to a combination of an adhesive and Cortisol. However, at 72 hours the cells exposed to combinations of OptiBond® with Cortisol and Prime & Bond® with Cortisol displayed predominantly spindle shaped cells compared to the predominantly round cells observed in the other samples. Cells exposed to a combination of adhesives with Nifedipine at 24 hour phase did not display any significant morphological alterations. In contrast, cells exposed to Prime & Bond® in combination with Nifedipine at the 48 hour phase revealed occasional morphological changes compared to control group. Likewise and at the end of 72 hour phase, the OptiBond®/Nifedipine and Prime & Bond®/Nifedipine groups displayed morphological differences such as clustering compared to the control. Biochemical analysis determined that there was a significant difference among the groups exposed to adhesives with Cortisol at 48 (Pdental adhesives and Cortisol at all phases. There were no differences in lactate dehydrogenase (LDH) levels among the groups exposed to combinations of adhesives and Cortisol or adhesives and Nifedipine. It is concluded that there were minor structural (morphological) alterations and major functional (biomarkers) changes observed upon exposing the gingival fibroblasts in

  15. Comparison of cytotoxicity among three gingival retraction cords on human gingival fibroblasts in vitro%三种排龈线对体外培养人牙龈成纤维细胞毒性的比较

    Institute of Scientific and Technical Information of China (English)

    倪莹; 张晓明; 惠敏; 刘健

    2012-01-01

    BACKGROUND: In recent years, the gingival retraction effect of gingival retraction drugs is a hot topic for domestic and foreign scholars, while the biocompatibility of the commercial gingival retraction cord is less involved. OBJECTIVE: To evaluate the cytotoxicity effect of three kinds of gingival retraction cords on human gingival fibroblasts, and to provide theoretical basis for choosing proper gingival retraction cords in clinic. METHODS: Three kinds of gingival retraction cords (epinephrine-impregnated cord, aluminium sulphate-impregnated cord and non-drug-impregnated cord) were incubated in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum for 24 hours, according to 1:1 and 1:4 dilution, and then the cords acted on gingival fibroblasts cultured in vitro. Dulbecco's modified Eagle's medium containing 10% fetal bovine serum was used as negative control group. RESULTS AND CONCLUSION: The results of MTT assay demonstrated that the three kinds of gingival retraction cords all could inhibit human gingival fibroblasts growth and proliferation. The cytotoxicity of the three kinds of gingival retraction cords in the leaching liquor with 1:1 dilution was significantly higher than that of 1:4 dilution and control groups (P< 0.05). The cytotoxicity of epinephrine-impregnated cord in the leaching liquor with 1:4 dilution was significantly higher than that of the control group (P < 0.05). The results of transmission electron microscope showed that some cells in the epinephrine-impregnated cord group were found apoptosis. These findings suggest that the three kinds of gingival retraction cords all have certain cytotoxicity to gingival fibroblasts. The cytotoxicity of epinephrine-impregnated cord is the biggest, followed by aluminium sulphate-impregnated cord, and the cytotoxicity of the non-drug-impregnated cord is the smallest.%背景:近年来,国内外学者研究热点主要集中在排龈药物的排龈效果,而对商品化排龈线的生物相

  16. Detection of (Leu-7)-positive cells with NK activity in human gingival tissues from patients with periodontitis

    Energy Technology Data Exchange (ETDEWEB)

    Komiyama, K.; Hirsch, H.Z.; Mestecky, J.; Moro, I.

    1986-03-05

    Natural killer (NK) cells have been identified in peripheral blood, lymphoid tissue and more recently in gut mucosa and may be involved in the regulation of immunoglobulin synthesis. They have assayed gingival tissues obtained from 25 periodontitis patients, for the presence and activity of NK cells. Routine histological techniques demonstrated an inflammatory infiltrate dominated by plasma cells and B lymphocytes. Indirect staining procedures with a biotin-labeled mouse anti-human, Leu-7 antibody revealed the presence of numerous positive cells accompanying the inflammatory cellular infiltrate in perivascular areas. Several specimens demonstrated positive-staining cells in the epithelium as well. Few cells were observed in histologically uninflammed areas. Single cell suspension obtained by collagenase digestion of 5 gingival samples were used in /sup 51/Cr release cytotoxicity assay against K562 cells. Three of the five samples were positive in this assay. The finding of Leu-7-positive cells in areas of intense plasma cell foci but not in uninflammed areas, may support a role for these cells in the regulation of immunoglobulin synthesis in oral mucosal tissues.

  17. Differential cytotoxicity of long-chain bases for human oral gingival epithelial keratinocytes, oral fibroblasts, and dendritic cells

    Directory of Open Access Journals (Sweden)

    Leslie A. Mehalick

    2015-12-01

    Full Text Available Long-chain bases, found in the oral cavity, have potent antimicrobial activity against oral pathogens. In an article associated with this dataset, Poulson and colleagues determined the cytotoxicities of long-chain bases (sphingosine, dihydrosphingosine, and phytosphingosine for human oral gingival epithelial (GE keratinocytes, oral gingival fibroblasts (GF, dendritic cells (DC, and squamous cell carcinoma (SCC cell lines [1]. Poulson and colleagues found that GE keratinocytes were more resistant to long-chain bases as compared to GF, DC, and SCC cell lines [1]. In this study, we assess the susceptibility of DC to lower concentrations of long chain bases. 0.2–10.0 µM long-chain bases and GML were not cytotoxic to DC; 40.0–80.0 µM long-chain bases, but not GML, were cytotoxic for DC; and 80.0 µM long-chain bases were cytotoxic to DC and induced cellular damage and death in less than 20 mins. Overall, the LD50 of long-chain bases for GE keratinocytes, GF, and DC were considerably higher than their minimal inhibitory concentrations for oral pathogens, a finding important to pursuing their future potential in treating periodontal and oral infections.

  18. Essential Oils from Ugandan Medicinal Plants: In Vitro Cytotoxicity and Effects on IL-1β-Induced Proinflammatory Mediators by Human Gingival Fibroblasts

    Science.gov (United States)

    Bwanga, Freddie; Joloba, Moses; Borg-Karlson, Anna-Karin; Yucel-Lindberg, Tülay; Obua, Celestino

    2016-01-01

    The study investigated cytotoxicity of essential oils from four medicinal plants (Bidens pilosa, Ocimum gratissimum, Cymbopogon nardus, and Zanthoxylum chalybeum) on human gingival fibroblasts and their effects on proinflammatory mediators' secretion. Cytotoxicity of essential oils was investigated using 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay. Effects of essential oils at subcytotoxicity concentrations on interleukin- (IL-) 6, IL-8, and prostaglandin E2 (PGE2) secretions by gingival fibroblasts treated with IL-1β (300 pg/mL) were evaluated by ELISA and EIA. IC50 values of the essential oils ranged from 26 μg/mL to 50 μg/mL. Baseline and IL-1β-induced secretion of PGE2 was inhibited by treatment with essential oil from O. gratissimum. Essential oils from B. pilosa and C. nardus had synergistic effects with IL-1β on PGE2 seceretion. In conclusion, the study suggests that essential oil from O. gratissimum decreases gingival fibroblasts secretion of PGE2, while essential oils from B. pilosa and C. nardus increase PGE2 secretion. Essential oil from Z. chalybeum was the most cytotoxic, while oil from C. nardus was the least cytotoxic. Although the clinical significance of these findings remains to be determined, it may be suggested that essential oil from O. gratissimum, applied at subcytotoxicity concentrations, could reduce the participation of gingival fibroblasts in the gingival inflammation and tissue destruction associated with periodontitis. PMID:27807462

  19. Essential Oils from Ugandan Medicinal Plants: In Vitro Cytotoxicity and Effects on IL-1β-Induced Proinflammatory Mediators by Human Gingival Fibroblasts

    Directory of Open Access Journals (Sweden)

    Francis Ocheng

    2016-01-01

    Full Text Available The study investigated cytotoxicity of essential oils from four medicinal plants (Bidens pilosa, Ocimum gratissimum, Cymbopogon nardus, and Zanthoxylum chalybeum on human gingival fibroblasts and their effects on proinflammatory mediators’ secretion. Cytotoxicity of essential oils was investigated using 3-(4, 5-dimethylthiazol-2-yl-2,5-diphenyl-tetrazolium bromide assay. Effects of essential oils at subcytotoxicity concentrations on interleukin- (IL- 6, IL-8, and prostaglandin E2 (PGE2 secretions by gingival fibroblasts treated with IL-1β (300 pg/mL were evaluated by ELISA and EIA. IC50 values of the essential oils ranged from 26 μg/mL to 50 μg/mL. Baseline and IL-1β-induced secretion of PGE2 was inhibited by treatment with essential oil from O. gratissimum. Essential oils from B. pilosa and C. nardus had synergistic effects with IL-1β on PGE2 seceretion. In conclusion, the study suggests that essential oil from O. gratissimum decreases gingival fibroblasts secretion of PGE2, while essential oils from B. pilosa and C. nardus increase PGE2 secretion. Essential oil from Z. chalybeum was the most cytotoxic, while oil from C. nardus was the least cytotoxic. Although the clinical significance of these findings remains to be determined, it may be suggested that essential oil from O. gratissimum, applied at subcytotoxicity concentrations, could reduce the participation of gingival fibroblasts in the gingival inflammation and tissue destruction associated with periodontitis.

  20. Essential Oils from Ugandan Medicinal Plants: In Vitro Cytotoxicity and Effects on IL-1β-Induced Proinflammatory Mediators by Human Gingival Fibroblasts.

    Science.gov (United States)

    Ocheng, Francis; Bwanga, Freddie; Almer Boström, Elisabeth; Joloba, Moses; Borg-Karlson, Anna-Karin; Yucel-Lindberg, Tülay; Obua, Celestino; Gustafsson, Anders

    2016-01-01

    The study investigated cytotoxicity of essential oils from four medicinal plants (Bidens pilosa, Ocimum gratissimum, Cymbopogon nardus, and Zanthoxylum chalybeum) on human gingival fibroblasts and their effects on proinflammatory mediators' secretion. Cytotoxicity of essential oils was investigated using 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay. Effects of essential oils at subcytotoxicity concentrations on interleukin- (IL-) 6, IL-8, and prostaglandin E2 (PGE2) secretions by gingival fibroblasts treated with IL-1β (300 pg/mL) were evaluated by ELISA and EIA. IC50 values of the essential oils ranged from 26 μg/mL to 50 μg/mL. Baseline and IL-1β-induced secretion of PGE2 was inhibited by treatment with essential oil from O. gratissimum. Essential oils from B. pilosa and C. nardus had synergistic effects with IL-1β on PGE2 seceretion. In conclusion, the study suggests that essential oil from O. gratissimum decreases gingival fibroblasts secretion of PGE2, while essential oils from B. pilosa and C. nardus increase PGE2 secretion. Essential oil from Z. chalybeum was the most cytotoxic, while oil from C. nardus was the least cytotoxic. Although the clinical significance of these findings remains to be determined, it may be suggested that essential oil from O. gratissimum, applied at subcytotoxicity concentrations, could reduce the participation of gingival fibroblasts in the gingival inflammation and tissue destruction associated with periodontitis.

  1. Evaluation in vitro of cytotoxicity of dentinal desensitizing on human gingival fibroblasts.

    Directory of Open Access Journals (Sweden)

    Diego Antonio Vergara

    2015-02-01

    Full Text Available ABSTRACT Introduction: The purpose of this study is to compare the cytotoxic effect of three materials which have been used for the treatment of dental hypersensitivity. Material and method: In-vitro study. Dentinal desensitizing Clinpro (3M ESPE, Seal&Protect (Dentsply and UltraEZ (Ultradent were used at concentrations of 0.1; 0.05; 0.01 and 0.001 g/ml against human gingival fibroblasts. Furthermore, Clinpro and Seal&Protect were applied to this cell culture as polymerized discs. Toxicity was assessed at 24 and 48 hours by cell viability assay (MTT. Statistical analysis for cell viability was performed using ANOVA two-ways and Tukey's post hoc test. Statistical significance was set at 5%. Results: Seal&Protect and Clinpro happened to be highly toxic at 24 and 48 hours, reaching 70% toxicity at concentrations over 0,01 g/ml. Seal&Protect and Clinpro polymerized discs were toxic after 24 and 48 hours. UltraEZ was increase of the cell viability between a 46% and 67% at 24 hours and between an 8% and 45% at 48 hours. Statistical analysis showed differences between these three desensitizing comparing concentration and control group (p RESUMEN. Introducción: El propósito de este estudio es comparar el efecto citotóxico de tres materiales que se han utilizado para el tratamiento de la hipersensibilidad dental. Material y método: Estudio in- vitro. Los desensibilizantes dentinarios Clinpro (3M ESPE, Seal&Protect (Dentsply y UltraEZ (Ultradent fueron utilizados a concentraciones de 0,1; 0,05; 0,01 y 0,001 g/ml sobre cultivos celulares de fibroblastos gingivales humanos. Además, Clinpro y Seal&Protect se aplicaron a este cultivo celular como discos polimerizados. La toxicidad se evaluó a 24 y 48 horas mediante ensayo de viabilidad (MTT. El análisis estadístico para la viabilidad celular se realizó mediante ANOVA de dos vías seguido de análisis Tukey. La significancia estadística se fijó al 5%. Resultados: Clinpro y Seal&Protect resultaron

  2. Crevicular Fluid and Serum Concentrations of Progranulin and High Sensitivity CRP in Chronic Periodontitis and Type 2 Diabetes

    Directory of Open Access Journals (Sweden)

    N. Priyanka

    2013-01-01

    Full Text Available Introduction. This study was designed to correlate the serum and gingival crevicular fluid (GCF levels of progranulin (PGRN and high sensitivity C-reactive protein (hs CRP in chronic periodontitis and type 2 diabetes mellitus (DM. Design. PGRN and hs CRP levels were estimated in 3 groups: healthy, chronic periodontitis, and type 2 DM with chronic periodontitis. Results. The mean PGRN and hs CRP concentrations in serum and GCF were the highest for group 3 followed by group 2 and the least in group 1. Conclusion. PGRN and hs CRP may be biomarkers of the inflammatory response in type 2 DM and chronic periodontitis.

  3. TLR expression profile of human gingival margin-derived stem progenitor cells

    Science.gov (United States)

    Mekhemar, Mohamed; Adam-Klages, Sabine; Kabelitz, Dietrich; Dörfer, Christof

    2016-01-01

    Background Gingival margin-derived stem/progenitor cells (G-MSCs) show remarkable periodontal regenerative potential in vivo. During regeneration, G-MSCs may interact with their inflammatory environment via toll-like-receptors (TLRs). The present study aimed to depict the G-MSCs TLRs expression profile. Material and Methods Cells were isolated from free gingival margins, STRO-1-immunomagnetically sorted and seeded to obtain single colony forming units (CFUs). G-MSCs were characterized for CD14, CD34, CD45, CD73, CD90, CD105, CD146 and STRO-1 expression, and for multilineage differentiation potential. Following G-MSCs’ incubation in basic or inflammatory medium (IL-1β, IFN-γ, IFN-α, TNF-α) a TLR expression profile was generated. Results G-MSCs showed all stem/progenitor cells’ characteristics. In basic medium G-MSCs expressed TLRs 1, 2, 3, 4, 5, 6, 7, and 10. The inflammatory medium significantly up-regulated TLRs 1, 2, 4, 5, 7 and 10 and diminished TLR 6 (p≤0.05, Wilcoxon-Signed-Ranks-Test). Conclusions The current study describes for the first time the distinctive TLRs expression profile of G-MSCs under uninflamed and inflamed conditions. Key words:Stem cells, TLR, gingiva, polymerase chain reaction, FACS. PMID:26615501

  4. Pharmacological and toxicological effects of co-exposure of human gingival fibroblasts to silver nanoparticles and sodium fluoride

    Science.gov (United States)

    Inkielewicz-Stepniak, Iwona; Santos-Martinez, Maria Jose; Medina, Carlos; Radomski, Marek W

    2014-01-01

    Background Silver nanoparticles (AgNPs) and fluoride (F) are pharmacological agents widely used in oral medicine and dental practice due to their anti-microbial/anti-cavity properties. However, risks associated with the co-exposure of local cells and tissues to these xenobiotics are not clear. Therefore, we have evaluated the effects of AgNPs and F co-exposure on human gingival fibroblast cells. Methods Human gingival fibroblast cells (CRL-2014) were exposed to AgNPs and/or F at different concentrations for up to 24 hours. Cellular uptake of AgNPs was examined by transmission electron microscopy. Downstream inflammatory effects and oxidative stress were measured by real-time quantitative polymerase chain reaction (PCR) and reactive oxygen species (ROS) generation. Cytotoxicity and apoptosis were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and real-time quantitative PCR and flow cytometry, respectively. Finally, the involvement of mitogen-activated protein kinases (MAPK) was studied using Western blot. Results We found that AgNPs penetrated the cell membrane and localized inside the mitochondria. Co-incubation experiments resulted in increased oxidative stress, inflammation, and apoptosis. In addition, we found that co-exposure to both xenobiotics phosphorylated MAPK, particularly p42/44 MAPK. Conclusion A combined exposure of human fibroblasts to AgNPs and F results in increased cellular damage. Further studies are needed in order to evaluate pharmacological and potentially toxicological effects of AgNPs and F on oral health. PMID:24729703

  5. Gingival changes during pregnancy: III. Impact of clinical, microbiological, immunological and socio-demographic factors on gingival inflammation.

    Science.gov (United States)

    Carrillo-de-Albornoz, Ana; Figuero, Elena; Herrera, David; Cuesta, Pedro; Bascones-Martínez, Antonio

    2012-03-01

    To identify predictor variables involved in exacerbated gingival inflammation associated with pregnancy. In this cohort study, 48 pregnant and 28 non-pregnant women without periodontitis were included. The pregnant women were evaluated in the first, second and third trimester and at 3 months postpartum, whilst the non-pregnant women were evaluated twice, with a 6-month interval. At each visit, clinical [plaque index (PlI) and gingival index (GI)], hormonal (salivary progesterone and estradiol), immunological [gingival crevicular fluid interleukin-1β, interleukin-6, tumour necrosis factor-α (TNF-α) and prostaglandin-E(2) ] and microbiological (periodontal pathogens culture) evaluations were performed. Statistical analysis was undertaken using exhaustive chi-square automatic interaction detection (exhaustive CHAID) to analyse the predictive value of the independent outcomes to develop pregnancy GI. PlI was the strongest predictor implicated in the GI throughout pregnancy and after delivery. During the second and third trimesters the presence of Porphyromonas gingivalis significantly contributed to the worsening of gingival inflammation. When compared with the non-pregnant group, significant differences were found in TNF-α amounts and concentrations and in the third trimester site-specific GI. Bacterial challenge to the gingival tissues, both quantitatively (PlI) and qualitatively (harbouring P. gingivalis) appears to affect the level of gingival inflammation observed during pregnancy. © 2011 John Wiley & Sons A/S.

  6. HIV阳性的牙周炎患者CD4+ T淋巴细胞计数和龈沟液前列腺素E2与牙周临床指标的相关性分析%Association of CD4+ T lymphocyte count and gingival crevicular fluid prostaglandin E2 with periodontal parameters in HIV-positive periodontitis patients

    Institute of Scientific and Technical Information of China (English)

    贾洪诚; 王璇; 华文浩; 李晓光; 侯雯; 付茜

    2014-01-01

    目的 评估HIV+牙周炎患者的CD4+ T淋巴细胞计数(简称CD4计数)和龈沟液中前列腺素E2(prostaglandin E2,PGE2)与牙周临床指标的相关性,以期为临床提供参考.方法 选择符合纳入标准的20例HIV+牙周炎患者,计数其血液CD4+T淋巴细胞.按CD4计数分组:A组(CD4计数< 200个/mm3,共计5例30颗牙)、B组(CD4计数为200~500个/mm3,共计13例78颗牙)、C组(CD4计数>500个/mm3,共计2例12颗牙),对受试者共计120颗指数牙进行牙周检查:菌斑指数(plaque index,PLI)、出血指数(bleeding index,BI)、附着水平(attachment level,AL)、牙周探诊深度(probing depth,PD),用放射免疫法检测龈沟液中PGE2.组间牙周临床指标和PGE2的比较采用Mann-Whitney秩和检验,CD4计数和牙周临床指标的相关性采用偏相关分析,PGE2与牙周临床指标的关系采用Spearman相关分析.结果 B组的BI值、PGE2质量浓度、PGE2总量分别为3.00(2.00)、90.75(30.60) μg/L及447.58(243.08) pg,均显著高于A组[分别为2.00(1.25)、79.75 (30.50) μg/L、339.52(200.97) pg]和C组[分别为2.00(1.00)、73.38(14.83) μg/L、299.18(108.33) pg](P<0.0167),而A组和C组的各项牙周临床指标差异及三组两两之间的PD和AL差异均无统计学意义(P>0.0167).A组和B组的CD4计数与BI有相关性,偏相关系数分别为0.657(P <0.05)、-0.369(P<0.05);C组的CD4计数与BI无相关性(P>0.05).PGE2质量浓度与探诊深度、附着丧失呈负相关(P<0.05),PGE2总量与各项牙周临床指标呈正相关(P<0.05).结论 HIV+牙周炎患者的牙周炎症程度与CD4计数有关,其龈沟液中PGE2水平与牙周临床指标有关.%Objective To investigate the correlation of CD4+ T lymphocyte count and prostaglandin E2 (PGE2) in gingival crevicular fluid(GCF) with periodontal status in HIV-positive patients with periodontitis.Methods Twenty subjects were selected according to inclusion criteria.The plasmatic CD4+ T lymphocytes were counted.All the

  7. Phagocytic activity of peripheral blood and crevicular phagocytes in health and periodontal disease

    Directory of Open Access Journals (Sweden)

    Asif K

    2010-01-01

    Full Text Available Background: Neutrophils constitute the main phagocytic cell system in mammalian host defense against an infecting agent. Abnormalities in leukocyte number and function are associated with increased susceptibility to periodontal diseases. The purpose of this study is to evaluate the in vitro phagocytic properties of crevicular and peripheral blood neutrophils in healthy and periodontitis subjects. Patients and Methods: A total of 30 subjects, that is, 10 patients in each of the following three groups: healthy controls, chronic periodontitis (CP, and localized aggressive periodontitis (LAP, were included in the study. The neutrophils were isolated from the peripheral blood and gingival crevice and tested for phagocytosis of Candida albicans. The percentage of leukocytes with ingested C. albicans was determined by light microscopy. Results: A significant reduction in the phagocytic activity of crevicular fluid polymorphonuclear neutrophils (CF-PMN of LAP subjects (mean: 54.3±7(P< 0.001 was observed, compared to healthy controls (mean: 74.2±9 and chronic periodontitis subjects (mean: 69±9(P=0.352. The mean percentage of peripheral blood polymorphonuclear neutrophils (PMNs with phagocytosis of opsonized C. albicans in LAP patients was significantly reduced (mean: 74.9±5(P< 0.0068 compared to the phagocytic activity of neutrophils from controls (mean:82.1±3 and chronic periodontitis subjects (mean: 82.0±5(P=0.970. There was no significant reduction in the phagocytic activity of CF PMNs (mean: 69±9 (P=0.35 and peripheral blood PMNs (mean: 82.5(P=0.97 in the chronic periodontitis group when compared to the control group. Conclusion: The phagocytic activity of both crevicular and peripheral neutrophils in subjects with periodontitis is altered, increasing the susceptibility to periodontitis. Thus individual susceptibility may be an additional and important modifying factor in the pathogenesis of periodontal disease.

  8. Changes of Substance P in the Crevicular Fluid in relation to Orthodontic Movement Preliminary Investigation

    Directory of Open Access Journals (Sweden)

    Luca Levrini

    2013-01-01

    Full Text Available Substance P (SP is a tachykinin released from both the central and the peripheral endings of primary afferent neurons and functions as a neurotransmitter. As a transmitter signaling pain, substance P is involved in nociception and is an extremely potent vasodilator. We found several studies about this neuropeptide especially in relation to parodontology and a few orthodontic reviews. This is because in the past the importance of this neuropeptide in dental element undergoing periodontal inflammation was observed. The aims of the present pilot study was to investigate whether the substance P was present in gingival crevicular fluid in dental elements undergoing orthodontic treatment with Invisalign technique compared to teeth belonging to the same series but not undergoing orthodontic movement. We analysed gengival crevicular fluid (GCF collected from four young subjects, using a paper cone for a time of 60 seconds. The results showed that SP is present in the gengival sulcus in elements undergoing orthodontic forces during treatment with Invisalign technique and not in the control teeth. During the literature analysis, we have found a lot of papers describing involvement of SP in periodontitis and inflammatory diseases, but further studies are needed in order to demonstrate the role of this neuropeptide during teeth movement.

  9. Changes of Substance P in the Crevicular Fluid in relation to Orthodontic Movement Preliminary Investigation

    Science.gov (United States)

    Levrini, Luca; Sacerdote, Paola; Moretti, Sarah; Panzi, Silvia; Caprioglio, Alberto

    2013-01-01

    Substance P (SP) is a tachykinin released from both the central and the peripheral endings of primary afferent neurons and functions as a neurotransmitter. As a transmitter signaling pain, substance P is involved in nociception and is an extremely potent vasodilator. We found several studies about this neuropeptide especially in relation to parodontology and a few orthodontic reviews. This is because in the past the importance of this neuropeptide in dental element undergoing periodontal inflammation was observed. The aims of the present pilot study was to investigate whether the substance P was present in gingival crevicular fluid in dental elements undergoing orthodontic treatment with Invisalign technique compared to teeth belonging to the same series but not undergoing orthodontic movement. We analysed gengival crevicular fluid (GCF) collected from four young subjects, using a paper cone for a time of 60 seconds. The results showed that SP is present in the gengival sulcus in elements undergoing orthodontic forces during treatment with Invisalign technique and not in the control teeth. During the literature analysis, we have found a lot of papers describing involvement of SP in periodontitis and inflammatory diseases, but further studies are needed in order to demonstrate the role of this neuropeptide during teeth movement. PMID:23737731

  10. [Gingival recessions and orthodontics].

    Science.gov (United States)

    Renkema, A M; Padmos, J A D; de Quincey, G de

    2015-11-01

    Gingival recessions represent the most visible periodontal disease. The prevalence of gingival recessions is high. The root surface is literally exposed to negative influences such as erosion, abrasion, discoloration and decay. Moreover, gingival recessions can affect the quality of life by increased thermal sensitivity and reduced dento-gingival aesthetics. The aetiology of gingival recessions is complex and considered to be multifactorial. In order to prevent the development of gingival recessions during and after orthodontic treatment, several factors should be taken into account, among which maintenance of optimal oral hygiene and respect for the 'biological envelope' are decisive. Once gingival recessions have developed, orthodontic therapy can play a positive role in their treatment.

  11. Presence of crevicular fluid Prostaglandin E2 in relation with clinical and radiographic periodontal status

    Directory of Open Access Journals (Sweden)

    Javier Elpidio Monzón

    2016-07-01

    Full Text Available Background and Objectives: Prostaglandin E2 (PGE2 is present in gingival crevicular fluid the (GCF and is evidenced in periodontal disease (PD. However, there are no enough reports to correlate the PGE2 concentrations in GCF in periodontal health and disease with clinical and radiographic indicators, age and gender. Hence, the present study is aimed to estimate the levels of PGE2 in GCF of subjects without periodontal disease (SEP and periodontal disease (CEP. Materials and Methods: 99 subjects were selected, 33 without PD (G1 and 66 with PD, 33 with gingivitis (G2 and 33 with periodontitis (G3, which were submitted to a clinical and radiographic diagnosis, registering samples FGC, being stored, centrifuged and refrigerated for preservation. Subsequently the concentration of crevicular PGE2 was measured by using the enzyme linked immunosorbent assay (ELISA, determining the concentration of each subject. Results: PGE2 was detected in all the samples. The G1 presented a concentration of 28.82 ± 2.88 pg / mL, G2 44.91 ± 4.37 pg / mL and G3 148.67 ± 74.74 pg / mL (0.0001. PGE2 levels were significantly correlated with bleeding on probing, probing depth, attachment loss and bone loss (0.05. PGE2 levels were modified by age, but not gender. Conclusion: It is well known that activated inflammatory cells produce inflammatory mediators that stimulate the production of PGE2. The findings of this study demonstrate an increased concentration of PGE2 in FCG according to the presence of greater severity of PD. PGE2 may be considered as a biomarker in PD progression. However, controlled, longitudinal studies are needed to confirm this possibility.

  12. Normal Human Gingival Epithelial Cells Sense C. parapsilosis by Toll-Like Receptors and Module Its Pathogenesis through Antimicrobial Peptides and Proinflammatory Cytokines

    Directory of Open Access Journals (Sweden)

    Raouf Bahri

    2010-01-01

    Full Text Available This study was designed to investigate the interaction between C. parapsilosis and human epithelial cells using monolayer cultures and an engineered human oral mucosa (EHOM. C. parapsilosis was able to adhere to gingival epithelial cells and to adopt the hyphal form in the presence of serum. Interestingly, when cultured onto the engineered human oral mucosa (EHOM, C. parapsilosis formed small biofilm and invaded the connective tissue. Following contact with C. parapsilosis, normal human gingival epithelial cells expressed high levels of Toll-like receptors (TLR-2, -4, and -6, but not TLR-9 mRNA. The upregulation of TLRs was paralleled by an increase of IL-1β, TNFα, and IFNγ mRNA expression, suggesting the involvement of these cytokines in the defense against infection with C. parapsilosis. The active role of epithelial cells in the innate immunity against C. parapsilosis infection was enhanced by their capacity to express high levels of human beta-defensin-1, -2, and -3. The upregulation of proinflammatory cytokines and antimicrobial peptide expression may explain the growth inhibition of C. parapsilosis by the gingival epithelial cells. Overall results provide additional evidence of the involvement of epithelial cells in the innate immunity against C. parapsilosis infections.

  13. Efeet of diferent inlay materials on the level of MMP-8 in gingival crevicular fluid%不同金属嵌体对龈沟液中基质金属蛋白酶-8水平的影响

    Institute of Scientific and Technical Information of China (English)

    靳松

    2009-01-01

    目的 通过检测嵌体修复患者龈沟液的量(gingival crevicutar fluid,GCF)、探诊深度(gingival crevicedepth,GCD)以及龈沟液基质金属蛋白酶-8(Matrix metalloproteinases-8,MMP-8)水平,比较镍铬合金和金铂合金铸造金属嵌体对牙周的影响.方法 选择临床病例20例,分别采用镍铬合金和金铂合金铸造金属嵌体修复后牙邻牙合Ⅱ类洞,分别在修复前、修复后12个月时检测修复牙GCF、GCD以及MMP-8水平.结果 12个月时镍铬合金组与金铂合金组在指标上差异有统计学意义(P<0.05).结论 非贵金属嵌体对牙周组织有不利影响.

  14. Extensive description and comparison of human supra-gingival microbiome in root caries and health.

    Directory of Open Access Journals (Sweden)

    Lin Chen

    Full Text Available Knowledge of the polymicrobial etiology of root caries is limited. To conduct a comprehensive research study on root caries, we utilized 454-pyrosequencing of 16S rRNA gene libraries and quantitative PCR to compare supra-gingival bacterial communities from healthy sites and carious sites of 21 patients with root caries (Patient-controls and Patient-cases and the sites of 21 healthy individuals (Healthy-controls from two nursing homes. Healthy-controls and Patient-cases showed no significant differences in terms of biomass, species richness, and species diversity. However, as for beta diversity based on either community membership metric (unweighted UniFrac or community structure metric (weighted UniFrac, Healthy-controls and Patient-cases were clearly distinguished from each other, appearing more variable in the community membership and structure in root caries microbiome but relatively conserved in the health microbiome. The Patient-controls group was at an intermediate stage between Healthy-controls and Patient-cases, but was more inclined to the former. Demonstrated in both relative abundance and prevalence of species in health and root caries, Propionibacterium acidifaciens, Streptococcus mutans, Olsenella profusa, Prevotella multisaccharivorax, and Lactobacillus crispatus were found to be most associated with root caries, whereas Delftia acidovorans, Bacteroidetes[G-2] sp., Lachnospiraceae[G-3] sp., and Prevotella intermedia are most associated with health. Our study provides a basis for further elucidating the microbial etiology of root caries in the elderly.

  15. Dentin bonding agents induce c-fos and c-jun protooncogenes expression in human gingival fibroblasts.

    Science.gov (United States)

    Huang, Fu-Mei; Chou, Ming-Yung; Chang, Yu-Chao

    2003-01-01

    An important requirement for a dentin bonding agent is biologic compatibility; the bonding agent usually remains in close contact with living dental tissues over a long period of time. Information on the genotoxicity/mutagenicity and cacinogenicity potentials of dentin bonding agents is rare. It has been shown that c-fos and c-jun are induced rapidly by a variety of chemical and physical stimuli. Little is known about the induction of cellular signaling events and specific gene expression after cell exposure to dentin bonding agents. Therefore, we used primary human gingival fibroblasts to examine the effect of six dentin bonding agents on the expression of c-fos and c-jun protooncogenes to evaluate the genotoxicity/mutagenicity and cacinogenicity potential of the dentin bonding agents. The levels of mRNA were measured by the quantitative RT-PCR analysis. c-fos and c-jun mRNA expression in dentin bonding agents-treated cells revealed a rapid accumulation of the transcript, a significant signal first was detectable after 1h of exposure. Persistent induction of c-jun and c-fos protooncogenes by dentine bonding agents may distribute systemically to cause some unexpected adverse effects on human beings. It would be necessary to identify the severely toxic compounds and replace these substances by better biocompatible components. Otherwise, leaching of those genotoxicity/mutagenicity and cacinogenicity components must be minimized or prevented.

  16. Characterization of arecoline-induced effects on cytotoxicity in normal human gingival fibroblasts by global gene expression profiling.

    Science.gov (United States)

    Chiang, Shang-Lun; Jiang, Shih-Sheng; Wang, Yi-Jou; Chiang, Horn-Che; Chen, Ping-Ho; Tu, Hung-Pin; Ho, Kun-Yen; Tsai, Yu-Shan; Chang, I-Shou; Ko, Ying-Chin

    2007-11-01

    Areca nut is the most widely used psychoactive substance and an important environmental risk factor for development of oral premalignant lesions and cancer. Arecoline, the major alkaloid of areca nut, has been known to cause cytotoxicity and genotoxicity in mammalian cells in vivo and in vitro and even contributes to carcinogenicity. However, the susceptible genes accounting for arecoline-induced damage in normal human oral cells are still lacking, which possibly involves in initial molecular damage via alternation of gene expression level on biological pathways. The present study was undertaken to characterize the toxic effects of arecoline in gene expression profiling on normal human gingival fibroblasts (HGF) using cDNA microarray and quantitative real-time reverse transcription PCR. The cytotoxicity of arecoline on HGF-1 cell line was elevated in a dose-dependent manner (p arecoline determined from dose-response curve of the cytotoxicity, a large number of genes were significantly repressed than induced by arecoline in global gene expression profiling. Five induced- and seven repressed genes including glutathione synthetase were further validated, and their gene expression changes were increased in a dose-dependent manner in a concentration range of 50-150 microg/ml. In conclusion, we proposed a tentative model to explain arecoline-induced effects on contribution of oral pathogenesis. The findings identified that 12 susceptible genes can potentially serve as biomarkers of arecoline-induced damage in betel chewers.

  17. Effect of Antimicrobial Peptide KSL-W on Human Gingival Tissue and C. albicans Growth, Transition and Secreted Aspartyl Proteinase (SAPS) 2, 4, 5 and 6 Expressions

    Science.gov (United States)

    2014-04-01

    fungi resistance , antimicrobial peptides, cationic peptides, chemical peptides, KSL-W. 3-ACCOMPLISHMENTS: There was no change as to the original...1 AWARD NUMBER: W81XWH-12-2-0025 TITLE: Effect of Antimicrobial Peptide KSL-W on Human Gingival Tissue and C. albicans Growth, Transition...of the Army position, policy or decision unless so designated by other documentation. REPORT DOCUMENTATION PAGE Form Approved OMB No. 0704-0188

  18. Effects of tetracyclines on neutrophil, gingival, and salivary collagenases. A functional and western-blot assessment with special reference to their cellular sources in periodontal diseases.

    Science.gov (United States)

    Sorsa, T; Ding, Y; Salo, T; Lauhio, A; Teronen, O; Ingman, T; Ohtani, H; Andoh, N; Takeha, S; Konttinen, Y T

    1994-09-06

    The characterization and regulation of matrix metalloproteinases (MMPs) have been studied to determine their role(s) in periodontal tissue destruction. Progress in elucidating the roles of MMPs in periodontal tissue destruction has led to a new concept involving the chemotherapeutic inhibition on MMPs, a therapeutic strategy which less than a decade ago was considered "a difficult and perhaps impossible task." Tetracyclines/doxycycline (DOXY) and their chemically modified nonantimicrobial derivatives (CMTs) are known to inhibit the matrix metalloproteinases, especially preferring human neutrophil collagenase (MMP-8), and prevent the oxidative activation of procollagenases. We characterized by Western blotting the molecular forms and cellular sources of gingival tissue, dental plaque, gingival crevicular fluid (GCF), and salivary MMPs associated with periodontitis. Also the molecular forms of tissue inhibitors of matrix metalloproteinases (TIMP-1 and TIMP-2) in periodontitis were studied by Western blot. Neutrophil (PMN)-derived MMPs were found to predominate in periodontitis, and phospholipase C present in increased amounts in periodontitis sites was found to be a potential inducer of PMN degranulation. We further studied the effects of DOXY on molecular forms of different latent and active MMPs purified from different cellular sources (PMNs, fibroblasts, keratinocytes) and present in vivo in oral exudates (gingival extracts, GCF, and saliva). DOXY inhibition of activated (oxidatively or proteolytically) MMPs were not associated with MMP fragmentation. Michaelis-Menten plots of initial rates of degradation of soluble type I collagen revealed an apparent Km value of 0.3-0.6 microM for MMP-8, and 75 microM DOXY inhibited MMP-8 in a manner which did not result in changes in apparent Km value but did prevent the initial degradation reaching Vmax providing evidence for noncompetitive inhibition. Treatment of patients with long-term DOXY medication results in decreased

  19. Effect of a dentifrice containing aloe vera on plaque and gingivitis control: a double-blind clinical study in humans

    Directory of Open Access Journals (Sweden)

    Sílvia Morgana Araújo de Oliveira

    2008-08-01

    Full Text Available The effect of Aloe vera on the reduction of plaque and gingivitis was evaluated in a randomized, parallel and double-blind clinical trial. Subjects were randomly allocated to the test group (n=15 - dentifrice containing Aloe vera - or the control group (n=15 - fluoridated dentifrice. Plaque index (PI and gingival bleeding index (GBI were assessed at days 0 and 30. Subjects were asked to brush their teeth with the control or test dentifrice, three times a day, during a 30-day period. There was a significant reduction on plaque and gingivitis in both groups, but no statistically significant difference was observed among them (p>0.01. The dentifrice containing Aloe vera did not show any additional effect on plaque and gingivitis control compared to the fluoridated dentifrice.

  20. EFFECT OF A DENTIFRICE CONTAINING ALOE VERA ON PLAQUE AND GINGIVITIS CONTROL. A DOUBLE-BLIND CLINICAL STUDY IN HUMANS

    Science.gov (United States)

    de Oliveira, Sílvia Morgana Araújo; Torres, Ticiana Carneiro; Pereira, Sérgio Luís da Silva; Mota, Olívia Morais de Lima; Carlos, Márlio Ximenes

    2008-01-01

    The effect of Aloe vera on the reduction of plaque and gingivitis was evaluated in a randomized, parallel and double-blind clinical trial. Subjects were randomly allocated to the test group (n=15) – dentifrice containing Aloe vera - or the control group (n=15) – fluoridated dentifrice. Plaque index (PI) and gingival bleeding index (GBI) were assessed at days 0 and 30. Subjects were asked to brush their teeth with the control or test dentifrice, three times a day, during a 30-day period. There was a significant reduction on plaque and gingivitis in both groups, but no statistically significant difference was observed among them (p>0.01). The dentifrice containing Aloe vera did not show any additional effect on plaque and gingivitis control compared to the fluoridated dentifrice. PMID:19089263

  1. Whole cigarette smoke increased the expression of TLRs, HBDs, and proinflammory cytokines by human gingival epithelial cells through different signaling pathways.

    Directory of Open Access Journals (Sweden)

    Abdelhabib Semlali

    Full Text Available The gingival epithelium is becoming known as a regulator of the oral innate immune responses to a variety of insults such as bacteria and chemicals, including those chemicals found in cigarette smoke. We investigated the effects of whole cigarette smoke on cell-surface-expressed Toll-like receptors (TLR-2, -4 and -6, human β-defensin (HBD and proinflammatory cytokine expression and production in primary human gingival epithelial cells. Whole cigarette smoke was shown to increase TLR2, TLR4 and TLR6 expression. Cigarette smoke led to ERK1/2, p38 and JNK phosphorylation in conjunction with nuclear factor-κB (NFκB translocation into the nucleus. TLR expression following cigarette smoke exposure was down regulated by the use of ERK1/2, p38, JNK MAP kinases, and NFκB inhibitors, suggesting the involvement of these signaling pathways in the cellular response against cigarette smoke. Cigarette smoke also promoted HBD2, HBD3, IL-1β, and IL-6 expression through the ERK1/2 and NFκB pathways. Interestingly, the modulation of TLR, HBD, and cytokine expression was maintained long after the gingival epithelial cells were exposed to smoke. By promoting TLR, HBDs, and proinflammatory cytokine expression and production, cigarette smoke may contribute to innate immunity dysregulation, which may have a negative effect on human health.

  2. Whole Cigarette Smoke Increased the Expression of TLRs, HBDs, and Proinflammory Cytokines by Human Gingival Epithelial Cells through Different Signaling Pathways

    Science.gov (United States)

    Semlali, Abdelhabib; Witoled, Chmielewski; Alanazi, Mohammed; Rouabhia, Mahmoud

    2012-01-01

    The gingival epithelium is becoming known as a regulator of the oral innate immune responses to a variety of insults such as bacteria and chemicals, including those chemicals found in cigarette smoke. We investigated the effects of whole cigarette smoke on cell-surface-expressed Toll-like receptors (TLR)-2, −4 and −6, human β-defensin (HBD) and proinflammatory cytokine expression and production in primary human gingival epithelial cells. Whole cigarette smoke was shown to increase TLR2, TLR4 and TLR6 expression. Cigarette smoke led to ERK1/2, p38 and JNK phosphorylation in conjunction with nuclear factor-κB (NFκB) translocation into the nucleus. TLR expression following cigarette smoke exposure was down regulated by the use of ERK1/2, p38, JNK MAP kinases, and NFκB inhibitors, suggesting the involvement of these signaling pathways in the cellular response against cigarette smoke. Cigarette smoke also promoted HBD2, HBD3, IL-1β, and IL-6 expression through the ERK1/2 and NFκB pathways. Interestingly, the modulation of TLR, HBD, and cytokine expression was maintained long after the gingival epithelial cells were exposed to smoke. By promoting TLR, HBDs, and proinflammatory cytokine expression and production, cigarette smoke may contribute to innate immunity dysregulation, which may have a negative effect on human health. PMID:23300722

  3. Proliferación de células madres mesenquimales obtenidas de tejido gingival humano sobre una matriz de quitosano: estudio in vitro Proliferation of mesenchymal stem cells from human gingival tissue on chitosan scaffold: an in vitro study

    Directory of Open Access Journals (Sweden)

    B M Hernández

    2011-08-01

    medio de cultivo.Aim: The purpose of this study was to assess the proliferation of mesenchymal stem cells (MSCs from human gingival tissue on chitosan matrix. Methods: Experimental study in vitro. Gingival connective tissue samples were obtained from healthy volunteers from the maxillary tuberosity. The explants were minced and cultured on tissue culture dishes. MSC were characterized by flow cytometry using markers for CD34, CD45, CD73, CD90, CD105 and for differentiation into, adipogenic, osteogenic and chondrogenic lineages. The tissue differentiated was analyzed with light microscopy and evaluated by culture staining using Oil Red, Alizarin Red y Safranina O respectively. MSC from passage 5 were cultured with chitosan scaffold. Proliferation of MSC was analyzed with light microscopy and crystal violet staining. Results: MSCs were obtained from gingival explants, and developed the standard of the morphologic and phenotypic characterization as a stem cell. The MSC adopted a fibroblastoid morphology, adherence to plastic, confluence of 80% and over 90% were consistently positive for CD90, CD105, CD73 markers and under 10% were negative for hematopoietic markers CD34 and CD45 by flow cytometry analysis. The MSC cultured in presence of chitosan matrix proliferated, however complete medium, it was dissolved forming a gel structure. We also observed that at higher concentrations of chitosan, MSC has less density and growth. Chitosan matrix in presence of cell culture medium loses physical properties, dissolving and forming a non-transportable gel. Conclusions: Despite the existence of proliferation of MSCs from human gingival tissue with chitosan matrix, its ability to act as a cell carrier and scaffold is deficient, since its physical properties are altered, dissolving and forming a non-transportable gel in contact with cell culture medium.

  4. Variation in Salivary Parameters and its Correlation with Plaque and Gingival Status among 12 to 15 Years Schoolchildren of Rural and Urban Jaipur City in Winter and Summer Seasons

    OpenAIRE

    Gaur, Anupama; Anup, N.; Sharma, Rajesh

    2012-01-01

    ABSTRACT Background: Saliva circulating in the mouth at any given time is termed as whole saliva and comprises of mixtures of secretions from major and minor salivary glands and traces from gingival crevicular fluid. This saliva in the form of unstimulated/stimulated salivary parameters governs the overall homeostatic mechanism of mouth. Aim: To evaluate variation in salivary parameters and its correlation with plaque and gingival status among 12 to 15 years schoolchildren of rural and urban ...

  5. Cytotoxic effects of mineral trioxide aggregate, calcium enriched mixture cement, Biodentine and octacalcium pohosphate on human gingival fibroblasts

    Directory of Open Access Journals (Sweden)

    Eshagh

    2016-06-01

    Full Text Available Background. This in vitro study compared the effects of mineral trioxide aggregate (MTA, calcium enriched mixture (CEM cement, Biodentine (BD and octacalcium phosphate (OCP on the viability of human gingival fibroblasts (HGFs. Methods. After completion of the setting time of the materials under study, fibroblasts were placed in 24-well insert plates and 1 mg of each material was added to the respective wells. The plates were then incubated at 37°C. The inserts were removed at 24, 48 and 168 hours and 2,5-diphenyltetrazolium bromide was added to assess cytotoxicity via the MTT colorimetric assay. Data were analyzed at different time intervals using repeated-measures ANOVA, followed by the Bonferroni test at three levels of significance of P < 0.05, P < 0.01 and P < 0.001. Results. Cytotoxicity of the materials under study was not significantly different at 24 and 48 hours compared to the control group. However, at 168 hours, a significant difference was noted between MTA (P< 0.05 and Biodentine (P < 0.01 and the control group. Conclusion. Cytotoxicity of MTA, CEM, Biodentine and OCP against HGFs was similar to that of the control group at 24 and 48 hours. Over time, MTA and Biodentine exhibited less cytotoxicity than other materials.

  6. Selective responses of human gingival fibroblasts and bacteria on carbon fiber reinforced polyetheretherketone with multilevel nanostructured TiO2.

    Science.gov (United States)

    Wang, Xiao; Lu, Tao; Wen, Jin; Xu, Lianyi; Zeng, Deliang; Wu, Qianju; Cao, Lingyan; Lin, Shuxian; Liu, Xuanyong; Jiang, Xinquan

    2016-03-01

    The long-term success of dental implants relies not only on stable osseointegration but also on the integration of implant surfaces with surrounding soft tissues. In our previous work, titanium plasma immersion ion implantation (PIII) technique was applied to modify the carbon-fiber-reinforced polyetheretherketone (CFRPEEK) surface, constructing a unique multilevel TiO2 nanostructure thus enhancing certain osteogenic properties. However, the interactions between the modified surface and soft-tissue cells are still not clear. Here, we fully investigate the biological behaviors of human gingival fibroblasts (HGFs) and oral pathogens on the structured surface, which determine the early peri-implant soft tissue integration. Scanning electron microscopy (SEM) shows the formation of nanopores with TiO2 nanoparticles embedded on both the sidewall and bottom. In vitro studies including cell adhesion, viability assay, wound healing assay, real-time PCR, western blot and enzyme-linked immunosorbent assay (ELISA) disclose improved adhesion, migration, proliferation, and collagen secretion ability of HGFs on the modified CFRPEEK. Moreover, the structured surface exhibits sustainable antibacterial properties towards Streptococcus mutans, Fusobacterium nucleatum and Porphyromonas gingivalis. Our results reveal that the multilevel TiO2 nanostructures can selectively enhance soft tissue integration and inhibit bacterial reproduction, which will further support and broaden the adoption of CFRPEEK materials in dental fields.

  7. Gold Nanoparticle-Mediated Delivery of Molecules into Primary Human Gingival Fibroblasts Using ns-Laser Pulses: A Pilot Study

    Directory of Open Access Journals (Sweden)

    Judith Krawinkel

    2016-05-01

    Full Text Available Interaction of gold nanoparticles (AuNPs in the vicinity of cells’ membrane with a pulsed laser (λ = 532 nm, τ = 1 ns leads to perforation of the cell membrane, thereby allowing extracellular molecules to diffuse into the cell. The objective of this study was to develop an experimental setting to deliver molecules into primary human gingival fibroblasts (pHFIB-G by using ns-laser pulses interacting with AuNPs (study group. To compare the parameters required for manipulation of pHFIB-G with those needed for cell lines, a canine pleomorphic adenoma cell line (ZMTH3 was used (control group. Non-laser-treated cells incubated with AuNPs and the delivery molecules served as negative control. Laser irradiation (up to 35 mJ/cm2 resulted in a significant proportion of manipulated fibroblasts (up to 85%, compared to non-irradiated cells: p < 0.05, while cell viability (97% was not reduced significantly. pHFIB-G were perforated as efficiently as ZMTH3. No significant decrease of metabolic cell activity was observed up to 72 h after laser treatment. The fibroblasts took up dextrans with molecular weights up to 500 kDa. Interaction of AuNPs and a pulsed laser beam yields a spatially selective technique for manipulation of even primary cells such as pHFIB-G in high throughput.

  8. The effect of non-steroidal anti-inflammatory drugs on the metabolism of /sup 14/C-arachidonic acid by human gingival tissue in vitro

    Energy Technology Data Exchange (ETDEWEB)

    Elattar, T.M.; Lin, H.S.; Tira, D.E.

    1983-09-01

    We investigated the effect of non-steroidal anti-inflammatory drugs on prostaglandins (PGs) and 12-hydroxyeicosatetraenoic acid (12-HETE) formation by inflamed human gingival tissues. Gingival tissue homogenates were incubated with /sup 14/C-arachidonic acid in the presence of indomethacin, piroxicam, or ibuprofen, and the organic solvent extracts were chromatographed on silica gel plates with standards for radiometric assay. There was a significant negative trend between the doses (10(-7)-10(-3) M) of each of indomethacin, piroxicam, and ibuprofen, and the amounts of PGF2 alpha, PGE2, PGD2, and 15-keto-PGE2 produced. All three drugs have a significant inhibitory effect on PGs and 12-HETE production at 10(-3) M when compared with the control. The rank order effectiveness of the drugs, at 10(-3) M, on PG inhibition was indomethacin greater than piroxicam greater than ibuprofen, and on 12-HETE inhibition was indomethacin greater than ibuprofen greater than piroxicam.

  9. Temporal activation of anti- and pro-apoptotic factors in human gingival fibroblasts infected with the periodontal pathogen, Porphyromonas gingivalis: potential role of bacterial proteases in host signalling

    Directory of Open Access Journals (Sweden)

    Takehara Tadamichi

    2006-03-01

    Full Text Available Abstract Background Porphyromonas gingivalis is the foremost oral pathogen of adult periodontitis in humans. However, the mechanisms of bacterial invasion and the resultant destruction of the gingival tissue remain largely undefined. Results We report host-P. gingivalis interactions in primary human gingival fibroblast (HGF cells. Quantitative immunostaining revealed the need for a high multiplicity of infection for optimal infection. Early in infection (2–12 h, P. gingivalis activated the proinflammatory transcription factor NF-kappa B, partly via the PI3 kinase/AKT pathway. This was accompanied by the induction of cellular anti-apoptotic genes, including Bfl-1, Boo, Bcl-XL, Bcl2, Mcl-1, Bcl-w and Survivin. Late in infection (24–36 h the anti-apoptotic genes largely shut down and the pro-apoptotic genes, including Nip3, Hrk, Bak, Bik, Bok, Bax, Bad, Bim and Moap-1, were activated. Apoptosis was characterized by nuclear DNA degradation and activation of caspases-3, -6, -7 and -9 via the intrinsic mitochondrial pathway. Use of inhibitors revealed an anti-apoptotic function of NF-kappa B and PI3 kinase in P. gingivalis-infected HGF cells. Use of a triple protease mutant P. gingivalis lacking three major gingipains (rgpA rgpB kgp suggested a role of some or all these proteases in myriad aspects of bacteria-gingival interaction. Conclusion The pathology of the gingival fibroblast in P. gingivalis infection is affected by a temporal shift from cellular survival response to apoptosis, regulated by a number of anti- and pro-apoptotic molecules. The gingipain group of proteases affects bacteria-host interactions and may directly promote apoptosis by intracellular proteolytic activation of caspase-3.

  10. Using saliva nitrite and nitrate levels as a biomarker for drug induced gingival overgrowth

    Directory of Open Access Journals (Sweden)

    Erkan eSukuroglu

    2015-12-01

    Full Text Available Aim: Drug-induced gingival overgrowth has a multifactorial nature and the pathogenesis is still uncertain. It has been suggested that Nitric Oxide (NO might play a role in the pathogenesis of drug-induced gingival overgrowth due to the contribution of NO to immune response and matrix degradation. NO levels in biological fluids have been used as a diagnostic biomarker in many diseases. The aim of this study is to determine whether NO levels in plasma, saliva and gingival crevicular fluid (GCF can serve as a potential biomarker for the evaluation of drug-induced gingival overgrowth risk. Material and Methods: A total of 104 patients, receiving cyclosporine A (n=35, phenytoin (n=25, nifedipine (n=26 or diltiazem (n=18 participated in the study. The amount of gingival overgrowth was evaluated with two indices and was given as percentage. Periodontal clinical parameters including plaque index (PI, gingival index (GI, gingival bleeding time index (GBTI and probing depth (PD were also assessed. Saliva, GCF and plasma samples were obtained from each participants. Nitrite and nitrate levels in saliva, GCF and plasma were analyzed by Griess reagent. Results: Salivary nitrite and nitrate levels in responders were significantly higher than those in non-responders in only phenytoin group (p˂0.05. Nitrite and nitrate levels of gingival crevicular fluid and plasma did not significantly differ between responders and non-responders in all study groups (p˃0.05. Salivary nitrite levels exhibited a significant correlation with PD, GBTI, severity of gingival overgrowth (%GO and GCF volume (p˂0.05. Additionally, a strong positive correlation was detected between saliva and plasma nitrate levels (p˂0.005. However, both nitrite and nitrate levels in GCF and plasma demonstrated no significant correlation with clinical parameters, GO severity and GCF volume (p˃0.05.Conclusion: Salivary nitrite and nitrate levels could be used as periodontal disease biomarkers in

  11. [Gingival recessions and orthodontics

    NARCIS (Netherlands)

    Renkema, A.M.; Padmos, J.A.; Quincey, G. de

    2015-01-01

    Gingival recessions represent the most visible periodontal disease. The prevalence of gingival recessions is high. The root surface is literally exposed to negative influences such as erosion, abrasion, discoloration and decay. Moreover, gingival recessions can affect the quality of life by increase

  12. Increased Advanced Oxidation Protein Products Generation by Cyclosporine-A and Angiotensin II in Human Gingival Fibroblasts – Ex-vivo Study

    Science.gov (United States)

    Subbarayan, Rajasekaran; Ajitkumar, Supraja; Murugan Girija, Dinesh

    2017-01-01

    Introduction Cyclosporin-A (CsA), an immunosuppressant, induces renal fibrosis and Renin Angiotensin System (RAS) is known to play a major role. CsA has the potential to increase the oxidative stress; specifically through the Advanced Oxidation Protein Products (AOPP) which could possibly stimulate fibrosis. A similar type of pathology occurs even in the gingiva known as CsA Induced Gingival Overgrowth (CIGO). Aim This study was undertaken to estimate the AOPP generation by Human Gingival Fibroblasts (HGF) under the influence of CsA and Angiotensin II (Ang II). Materials and Methods Six healthy gingival tissue samples were obtained during crown lengthening procedure and primary HGF were cultured using enzymatic digestion method. The ideal non-cytotoxic concentrations of CsA and Ang II were identified using cytotoxicity assay. Later, HGF were incubated with CsA and Ang II for 12 hours and AOPP assay was performed at zero and one hour interval. Results There was a statistically significant increase in AOPP production in both the CsA and Ang II when compared to the control group with a p value<0.05. Conclusion CsA can induce oxidative stress and preventing/controlling it may be necessary to prevent untoward effect of the drug. PMID:28274044

  13. Comparative study on IL-1βand TNF-αin gingival crevicular fluid of peri-implants and teeth%IL-1β和TNF-α在种植体和天然牙周围龈沟液中的比较研究

    Institute of Scientific and Technical Information of China (English)

    黄仕禄; 牟雁东; 雍苓; 廖娟; 邓军

    2016-01-01

    目的:本研究旨在探讨健康种植体和天然牙周围龈沟液的IL-1β和TNF-α的水平,为维持种植体周围组织的健康提供理论依据。方法:本研究共纳入17名患者,每名患者含有一颗健康种植牙和一颗同名天然牙(种植牙组:17枚ITI种植体;天然牙组:17颗天然牙)。采用酶联免疫吸附法对健康种植体和天然牙周围龈沟液的IL-1β和TNF-α进行分析;采用Spearman法对两组的IL-1β和TNF-α的水平相关性进行分析。结果:与天然牙组相比较,种植牙组的IL-1β和TNF-α的水平均增加。种植牙组和天然牙组的IL-1β水平分别为(Mean±SD,26.14±10.26;21.93±6.43),但两组间的IL-1β水平无统计学差异(P>0.05);种植牙组和天然牙组的TNF-α水平分别为(Mean ±SD,3.17±0.72;2.67±0.58),两组间的TNF-α水平具有显著性统计学差异(P0.05). The levels of TNF-α of implants and teeth were re-spectively(Mean±SD, 3.17±0.72;2.67±0.58), and the difference of levels of TNF-α between two groups was statistically significant (P<0.05). Significant positive correlation was noted between the IL-1β and TNF-α in implants group(P<0.05), and in teeth group(P<0.05). Conclusion: The levels of IL-1β and TNF-α in gingival creviscular fluid of healthy implants were higher compared with the natural teeth. The composition and structure of connective tissue around the implants, plaque and the implant itself, may make more production of the IL-1β and TNF-α in gingival creviscular fluid around the healthy implants than teeth.

  14. 钴铬合金、纯钛及全瓷冠种植体周围龈沟液中ALP、AST的表达%Expression of aspartate aminotransferase and alkaline phosphatase in gingival crevicular fluid around implants with cobalt-chromium alloy, titanium and all-ceramic crowns

    Institute of Scientific and Technical Information of China (English)

    缪羽; 杨慧; 李佳; 于蕴之

    2015-01-01

    目的:探讨3种不同材料的全冠修复体在种植体周围组织龈沟液(GCF)、牙龈指数(gingival index,GI)、牙龈龈沟探诊深度(gingival crevice depth,GCD)变化及GCF中天冬氨酸转氨酶(AST)和碱性磷酸酶(ALP)水平,以评价不同烤瓷内冠材料对种植体周围牙周组织的影响.方法:经患者知情同意,随机选择临床病例90颗,平均分为3组,分别采用钴铬合金烤瓷冠,纯钛烤瓷冠,全瓷冠作为种植体上部修复材料,每组30颗.在修复前,后1个月、3个月,分别进行CGF量、GI、GCD、AST和ALP水平的测定和分析.结果:钴铬合金组、纯钛组、全瓷冠组术前龈沟液中各指标间差异均无统计学意义(P>0.05),钴铬合金组,纯钛组,全瓷冠组修复术前、后1个月,3个月龈沟液中GCF量、GI、GCD、AST、ALP各指标间差异均有统计学意义(P<0.05).术后1个月ALP、AST在钴铬合金组、全瓷组及纯钛组间差异均有统计学意义差别(P<0.05),而GCF、GI、GCD组间差异均无统计学意义(P>0.05);术后3个月,钴铬合金组、全瓷组、纯钛组各指标间差异均有统计学意义(P<0.05).GCF、GI、GCD、AST、ALP分别进行三组间两两比较,GCF、GCD、AST、ALP各对比组间差异均有统计学意义(P<0.05),但GI三组间两两比较全瓷组与钴铬合金组差异有统计学意义(P<0.05),与其他对比组差异均无统计学意义(P>0.05).结论:钴铬合金烤瓷冠可导致修复患牙的GCF量及其中酶的变化,对牙周组织有不利影响,纯钛烤瓷冠和全瓷冠对牙周组织的影响相对较小.

  15. 2型糖尿病伴牙周炎病人血清HbA1c、龈沟液IL-1β和TNF-α的检测%Serum HbA1c,gingival crevicular fluid IL-1β and TNF-α Detection in type 2 diabetic patients with periodontitis

    Institute of Scientific and Technical Information of China (English)

    王鑫; 高秀秋

    2010-01-01

    目的:研究2型糖尿病伴牙周炎病人、单纯牙周炎病人、牙周健康者龈沟液(gingival cervicular fluid,GCF)白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)水平及其关系.方法:选取单纯牙周炎病人35例,2型糖尿病伴牙周炎病人35例,另选取单纯健康者30例,检查并记录探诊出血阳性率、探诊深度和附着水平丧失.运用高效液相层析法、ELISA法、放射免疫法分别测定各组血清糖化血红蛋白(HbA1c)、龈沟液IL-1β和TNF-α的水平.结果:2型糖尿病合并牙周炎组血清糖化血红蛋白、龈沟液IL-1β和TNF-α的水平均明显高于单纯牙周炎组、正常对照组(P0.05),龈沟液中IL-1β和TNF-α水平均有显著性差异(P<0.05).结论:IL-1β、TNF-α可能在牙周炎对2型糖尿病的影响中起一定的作用.

  16. Effect of diabetes on levels of IL-1β and PGE2 in gingival crevicular fluid in patients with periodontitis%糖尿病对牙周炎病人龈沟液白介素-1β、前列腺素E2水平的影响

    Institute of Scientific and Technical Information of China (English)

    李长春; 张帆

    2009-01-01

    目的:探讨Ⅱ型糖尿病对牙周炎病人龈沟液白介素-1β(IL-1β)、前列腺素E2(PGE2)水平的影响及其与糖脂代谢情况的关系.方法:选择Ⅱ型糖尿病伴发牙周炎病人18例(DM组)、单纯牙周炎病人18例(PD组)和全身、牙周健康者18例(H组)为研究对象.分别测定各组糖化血红蛋白(HbA1c)、血脂水平以及龈沟液(gingival cervicularfluid GCF)中IL-1β、PGE2 水平,并同时测定牙周龈沟出血指数、探诊深度、附着丧失等指标.结果:DM、PD组龈沟出血指数(SBI)、探诊深度(PD)、附着丧失(AL)、龈沟液PGE2水平明显高于H组(P<0.05),DM与PD组无明显差异;DM组病人糖化血红蛋白、龈沟液IL-1β指标均明显高于PD、H组(P<0.05);龈沟液IL-1β水平与HbA1c含量正相关.结论:糖尿病伴发牙周炎病人龈沟液IL-1β水平升高可能受全身因素影响,进一步促进牙周病变发展.

  17. Vitamin D reduces the inflammatory response by Porphyromonas gingivalis infection by modulating human β-defensin-3 in human gingival epithelium and periodontal ligament cells.

    Science.gov (United States)

    De Filippis, Anna; Fiorentino, Margherita; Guida, Luigi; Annunziata, Marco; Nastri, Livia; Rizzo, Antonietta

    2017-04-03

    Periodontitis is a multifactorial polymicrobial infection characterized by a destructive inflammatory process. Porphyromonas gingivalis, a Gram-negative black-pigmented anaerobe, is a major pathogen in the initiation and progression of periodontitis; it produces several virulence factors that stimulate human gingival epithelium (HGE) cells and human periodontal ligament (HPL) cells to produce various inflammatory mediators. A variety of substances, such as vitamin D, have growth-inhibitory effects on some bacterial pathogens and have shown chemo-preventive and anti-inflammatory activity. We used a model with HGE and HPL cells infected with P. gingivalis to determine the influence of vitamin D on P. gingivalis growth and adhesion and the immunomodulatory effect on TNF-α, IL-8, IL-12 and human-β-defensin 3 production. Our results demonstrated, firstly, the lack of any cytotoxic effect on the HGE and HPL cells when treated with vitamin D; in addition, vitamin D inhibited P. gingivalis adhesion and infectivity in HGE and HPL cells. Our study then showed that vitamin D reduced TNF-α, IL-8, IL-12 production in P. gingivalis-infected HGE and HPL cells. In contrast, a significant upregulation of the human-β-defensin 3 expression in HGE and HPL cells induced by P. gingivalis was demonstrated. Our results indicate that vitamin D specifically enhances the production of the human-β-defensin 3 antimicrobial peptide and exerts an inhibitory effect on the pro-inflammatory cytokines, thus suggesting that vitamin D may offer possible therapeutic applications for periodontitis.

  18. Effects of miR-223 on expression of IL-1β and IL-6 in human gingival fibroblasts.

    Science.gov (United States)

    Matsui, Sari; Ogata, Yorimasa

    2016-01-01

    MicroRNAs (miRNAs) are small non-coding RNAs that regulate post-transcriptional expression by translational inhibition or mRNA degradation. miRNAs bind to target mRNAs through partial complementarity, and can regulate many genes. In the present study, we investigated the effects of miR-223 on the expression of inflammatory cytokines in human gingival fibroblasts (HGF). To determine the effects of miR-223 on the expressions of interleukin-1β (IL-1β) and IL-6, HGF were stimulated by IL-1β (1 ng/mL) or tumor necrosis factor-α (TNF-α; 10 ng/mL) and transfected with a miR-223 expression plasmid. Levels of mRNA for IL-1β, IL-6, inhibitor of kappa-B kinase α (IKKα) and mitogen-activated protein kinase phosphatase-5 (MKP-5) were measured by real-time PCR, and levels IL-1β, IL-6 and IKKα protein were determined by enzyme-linked immunosorbent assay and Western blotting. Expression of IL-1β and IL-6 mRNAs was induced by IL-1β and TNF-α and further increased by miR-223 overexpression. IL-1β and TNF-α induced the expression of IL-1β and IL-6 mRNAs, and this was reduced by miR-223 inhibitor. Overexpression of miR-223 decreased the levels of IKKα protein and MKP-5 mRNA in HGF. These findings indicate that miR-223 might control the inflammatory response via IKKα and MKP-5 in periodontal tissue. (J Oral Sci 58, 101-108, 2016).

  19. Generation and periodontal differentiation of human gingival fibroblasts-derived integration-free induced pluripotent stem cells

    Energy Technology Data Exchange (ETDEWEB)

    Yin, Xiaohui [Department of Periodontology, School and Hospital of Stomatology, Peking University, 22 South Avenue Zhong-Guan-Cun, Beijing 100081 (China); Peking University Stem Cell Research Center and Department of Cell Biology, School of Basic Medical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191 (China); Li, Yang [Peking University Stem Cell Research Center and Department of Cell Biology, School of Basic Medical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191 (China); Li, Jingwen [Department of Periodontology, School and Hospital of Stomatology, Peking University, 22 South Avenue Zhong-Guan-Cun, Beijing 100081 (China); Li, Peng [Faculty of Dentistry, The University of Hong Kong, 34 Hospital Road, Hong Kong SAR (China); Liu, Yinan [Peking University Stem Cell Research Center and Department of Cell Biology, School of Basic Medical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191 (China); Wen, Jinhua, E-mail: jhwen@bjmu.edu.cn [Peking University Stem Cell Research Center and Department of Cell Biology, School of Basic Medical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191 (China); Luan, Qingxian, E-mail: kqluanqx@126.com [Department of Periodontology, School and Hospital of Stomatology, Peking University, 22 South Avenue Zhong-Guan-Cun, Beijing 100081 (China)

    2016-05-06

    Induced pluripotent stem cells (iPSCs) have been recognized as a promising cell source for periodontal tissue regeneration. However, the conventional virus-based reprogramming approach is associated with a high risk of genetic mutation and limits their therapeutic utility. Here, we successfully generated iPSCs from readily accessible human gingival fibroblasts (hGFs) through an integration-free and feeder-free approach via delivery of reprogramming factors of Oct4, Sox2, Klf4, L-myc, Lin28 and TP53 shRNA with episomal plasmid vectors. The iPSCs presented similar morphology and proliferation characteristics as embryonic stem cells (ESCs), and expressed pluripotent markers including Oct4, Tra181, Nanog and SSEA-4. Additionally, these cells maintained a normal karyotype and showed decreased CpG methylation ratio in the promoter regions of Oct4 and Nanog. In vivo teratoma formation assay revealed the development of tissues representative of three germ layers, confirming the acquisition of pluripotency. Furthermore, treatment of the iPSCs in vitro with enamel matrix derivative (EMD) or growth/differentiation factor-5 (GDF-5) significantly up-regulated the expression of periodontal tissue markers associated with bone, periodontal ligament and cementum respectively. Taken together, our data demonstrate that hGFs are a valuable cell source for generating integration-free iPSCs, which could be sequentially induced toward periodontal cells under the treatment of EMD and GDF-5. - Highlights: • Integration-free iPSCs are successfully generated from hGFs via an episomal approach. • EMD promotes differentiation of the hGFs-derived iPSCs toward periodontal cells. • GDF-5 promotes differentiation of the hGFs-derived iPSCs toward periodontal cells. • hGFs-derived iPSCs could be a promising cell source for periodontal regeneration.

  20. Effects of Plasma Rich in Growth Factors and Platelet-Rich Fibrin on Proliferation and Viability of Human Gingival Fibroblasts

    Directory of Open Access Journals (Sweden)

    Surena Vahabi

    2016-01-01

    Full Text Available Objectives: Platelet preparations are commonly used to enhance bone and soft tissue regeneration. Considering the existing controversies on the efficacy of platelet products for tissue regeneration, more in vitro studies are required. The aim of the present study was to compare the in vitro effects of plasma rich in growth factors (PRGF and platelet-rich fibrin (PRF on proliferation and viability of human gingival fibroblasts (HGFs.Materials and Methods: Anitua's PRGF and Choukran's PRF were prepared according to the standard protocols. After culture periods of 24, 48 and 72 hours, proliferation of HGFs was evaluated by the methyl thiazol tetrazolium assay. Statistical analysis was performed using one-way ANOVA followed by Tukey-Kramer’s multiple comparisons and P-values<0.05 were considered statistically significant.Results: PRGF treatment induced statistically significant (P<0.001 proliferation of HGF cells compared to the negative control (100% viability at 24, 48 and 72 hours in values of 123%±2.25%, 102%±2.8% and 101%±3.92%, respectively. The PRF membrane treatment of HGF cells had a statistically significant effect on cell proliferation (21%±1.73%, P<0.001 at 24 hours compared to the negative control. However, at 48 and 72 hours after treatment, PRF had a negative effect on HGF cell proliferation and caused 38% and 60% decrease in viability and proliferation compared to the negative control, respectively. The HGF cell proliferation was significantly higher in PRGF than in PRF group (P< 0.001.Conclusion: This study demonstrated that PRGF had a strong stimulatory effect on HGF cell viability and proliferation compared to PRF.

  1. Cannabidiol Modulates the Immunophenotype and Inhibits the Activation of the Inflammasome in Human Gingival Mesenchymal Stem Cells.

    Science.gov (United States)

    Libro, Rosaliana; Scionti, Domenico; Diomede, Francesca; Marchisio, Marco; Grassi, Gianpaolo; Pollastro, Federica; Piattelli, Adriano; Bramanti, Placido; Mazzon, Emanuela; Trubiani, Oriana

    2016-01-01

    Human Gingival Mesenchymal Stem Cells (hGMSCs) are multipotential cells that can expand and differentiate in culture under specific and standardized conditions. In the present study, we have investigated whether in vitro pre-treatment of hGMSCs with Cannabidiol (CBD) can influence their expression profile, improving the therapeutic potential of this cell culture. Following CBD treatment (5 μM) for 24 h, gene expression analysis through Next Generation Sequencing (NGS) has revealed several genes differentially expressed between CBD-treated hGMSCs (CBD-hGMSCs) and control cells (CTR-hGMSCs) that were linked to inflammation and apoptosis. In particular, we have demonstrated that CBD treatment in hGMSCs prevented the activation of the NALP3-inflammasome pathway by suppressing the levels of NALP3, CASP1, and IL18, and in parallel, inhibited apoptosis, as demonstrated by the suppression of Bax. CBD treatment was also able to modulate the expression of the well-known mesenchymal stem cell markers (CD13, CD29, CD73, CD44, CD90, and CD166), and other surface antigens. Specifically, CBD led to the downregulation of genes codifying for antigens involved in the activation of the immune system (CD109, CD151, CD40, CD46, CD59, CD68, CD81, CD82, CD99), while it led to the upregulation of those implicated in the inhibition of the immune responses (CD47, CD55, CD276). In conclusion, the present study will provide a new simple and reproducible method for preconditioning hGMSCs with CBD, before transplantation, as an interesting strategy for improving the hGMSCs molecular phenotype, reducing the risk of immune or inflammatory reactions in the host, and in parallel, for increasing their survival and thus, their long-term therapeutic efficacy.

  2. Cannabidiol Modulates the Immunophenotype and Inhibits the Activation of the Inflammasome in Human Gingival Mesenchymal Stem Cells

    Science.gov (United States)

    Libro, Rosaliana; Scionti, Domenico; Diomede, Francesca; Marchisio, Marco; Grassi, Gianpaolo; Pollastro, Federica; Piattelli, Adriano; Bramanti, Placido; Mazzon, Emanuela; Trubiani, Oriana

    2016-01-01

    Human Gingival Mesenchymal Stem Cells (hGMSCs) are multipotential cells that can expand and differentiate in culture under specific and standardized conditions. In the present study, we have investigated whether in vitro pre-treatment of hGMSCs with Cannabidiol (CBD) can influence their expression profile, improving the therapeutic potential of this cell culture. Following CBD treatment (5 μM) for 24 h, gene expression analysis through Next Generation Sequencing (NGS) has revealed several genes differentially expressed between CBD-treated hGMSCs (CBD-hGMSCs) and control cells (CTR-hGMSCs) that were linked to inflammation and apoptosis. In particular, we have demonstrated that CBD treatment in hGMSCs prevented the activation of the NALP3-inflammasome pathway by suppressing the levels of NALP3, CASP1, and IL18, and in parallel, inhibited apoptosis, as demonstrated by the suppression of Bax. CBD treatment was also able to modulate the expression of the well-known mesenchymal stem cell markers (CD13, CD29, CD73, CD44, CD90, and CD166), and other surface antigens. Specifically, CBD led to the downregulation of genes codifying for antigens involved in the activation of the immune system (CD109, CD151, CD40, CD46, CD59, CD68, CD81, CD82, CD99), while it led to the upregulation of those implicated in the inhibition of the immune responses (CD47, CD55, CD276). In conclusion, the present study will provide a new simple and reproducible method for preconditioning hGMSCs with CBD, before transplantation, as an interesting strategy for improving the hGMSCs molecular phenotype, reducing the risk of immune or inflammatory reactions in the host, and in parallel, for increasing their survival and thus, their long-term therapeutic efficacy. PMID:27932991

  3. Cannabidiol modulates the immunophenotype and inhibits the activation of the inflammasome in human gingival mesenchymal stem cells

    Directory of Open Access Journals (Sweden)

    Rosaliana Libro

    2016-11-01

    Full Text Available Human Gingival Mesenchymal Stem Cells (hGMSC are multipotential cells that can expand and differentiate in culture under specific and standardized conditions. In the present study, we have investigated whether in vitro pre-treatment of hGMCs with Cannabidiol (CBD can influence their expression profile, improving the therapeutic potential of this cell culture. Following CBD treatment (5μM for 24 h, gene expression analysis through Next Generation Sequencing (NGS has revealed several genes differentially expressed between CBD-treated hGMCs (CBD-hGMCs and control cells (CTR-hGMCs that were linked to inflammation and apoptosis. In particular, we have demonstrated that CBD treatment in hGMCs prevented the activation of the NALP3-inflammasome pathway by suppressing the levels of NALP3, CASP1 and IL18, and in parallel, inhibited apoptosis, as demonstrated by the suppression of Bax.CBD treatment was also able to modulate the expression of the well-known mesenchymal stem cell markers (CD13, CD29, CD73, CD44, CD90 and CD166, and other surface antigens. Specifically, CBD led to the downregulation of genes codifying for antigens involved in the activation of the immune system (CD109, CD151, CD40, CD46, CD59, CD68, CD81, CD82, CD99, while it led to the upregulation of those implicated in the inhibition of the immune responses (CD47, CD55, CD276.In conclusion, the present study will provide a new simple and reproducible method for preconditioning hGMSCs with CBD, before transplantation, as an interesting strategy for improving the hGMCs molecular phenotype, reducing the risk of immune or inflammatory reactions in the host, and in parallel, for increasing their survival and thus, their long-term therapeutic efficacy.

  4. Cannabidiol Activates Neuronal Precursor Genes in Human Gingival Mesenchymal Stromal Cells.

    Science.gov (United States)

    Soundara Rajan, Thangavelu; Giacoppo, Sabrina; Scionti, Domenico; Diomede, Francesca; Grassi, Gianpaolo; Pollastro, Federica; Piattelli, Adriano; Bramanti, Placido; Mazzon, Emanuela; Trubiani, Oriana

    2016-12-05

    In the last years, mesenchymal stromal cells (MSCs) from oral tissues have received considerable interest in regenerative medicine since they can be obtained with minimal invasive procedure and exhibit immunomodulatory properties. This study was aimed to investigate whether in vitro pre-treatment of MSCs obtained from human gingiva (hGMSCs) with Cannabidiol (CBD), a cannabinoid component produced by the plant Cannabis sativa, may promote human gingiva derived MSCs to differentiate toward neuronal precursor cells. Specifically, we have treated the hGMSCs with CBD (5 µM) for 24 h in order to evaluate the expression of genes involved in cannabidiol signaling, cell proliferation, self-renewal and multipotency, and neural progenitor cells differentiation. Next generation sequencing (NGS) demonstrated that CBD activates genes associated with G protein coupled receptor signaling in hGMSCs. Genes involved in DNA replication, cell cycle, proliferation, and apoptosis were regulated. Moreover, genes associated with the biological process of neuronal progenitor cells (NCPs) proliferation, neuron differentiation, neurogenesis, and nervous system development were significantly modulated. From our results, we hypothesize that human gingiva-derived MSCs conditioned with CBD could represent a valid method for improving the hGMSCs phenotype and thus might be a potential therapeutic tool in the treatment of neurodegenerative diseases. J. Cell. Biochem. 9999: 1-16, 2016. © 2016 Wiley Periodicals, Inc.

  5. Effects of periodontal initial therapy on Matrix-metalloproteinases-9 levels in the unstimulated whole saliva, gingival crevicular fluid and serum of chronic periodontitis patients%牙周基础治疗对慢性牙周炎患者非刺激性全唾液、龈沟液及血清中基质金属蛋白酶-9水平的影响

    Institute of Scientific and Technical Information of China (English)

    柴琳; 詹渊博; 宋雪静; 徐晓满; 穆森; 张瑞敏

    2014-01-01

    目的 评估慢性牙周炎患者经牙周基础治疗前后的非刺激性全唾液、龈沟液及血清中基质金属蛋白酶-9 (MMp-9)水平,探讨其作为牙周炎诊断及预后标志物的可能性.方法 酶联免疫吸附试验(ELISA)检测20名牙周炎患者治疗前、后及20名健康人非刺激性全唾液、龈沟液及血清中MMP-9的水平,并记录牙周袋探诊深度(PD)、临床附着丧失(CAL)和出血指数(BI).结果 ①除CAL外,经牙周基础治疗6周后慢性牙周炎患者的临床指标明显改善[CAL治疗前(5.50±1.97) mm,治疗后(5.50±1.97) mm,P=1.000;PD治疗前(7.05±1.81) mm,治疗后(4.23±1.06) mm,P=0.000;BI治疗前3.75±0.44,治疗后0.20±0.41,P=0.000);②治疗后非刺激性全唾液、龈沟液及血清中MMP-9水平明显降低,与治疗前相比差异具有统计学意义[非刺激性全唾液MMP-9水平治疗前(22.89±5.28)ng/mL,治疗后(18.11±7.19) ng/mL,P=0.003;龈沟液MMP-9水平治疗前(41.80±2.90) ng/mL,治疗后(35.71±4.49) ng/mL,P=0.000;血清MMP-9水平治疗前(6.67±5.318) ng/mL,治疗后(2.47±2.713) ng/mL,P=0.004];③除血清外,牙周炎患者治疗前后的非刺激性全唾液和龈沟液中MMP-9水平仍高于健康对照组,其差异有统计学意义[非刺激性全唾液水平为(6.78±3.02)ng/mL,龈沟液为(30.20±3.64) ng/mL,与治疗前后比较P=0.000;健康对照组血清MMP-9水平(1.18±0.88) ng/mL,与治疗前、后比较P =0.004、P=0.055].结论 非刺激性全唾液中MMP-9的表达水平可能成为慢性牙周炎的检测指标之一,可能为临床诊治提供参考依据.%Objective To evaluate the MMP-9 levels in the unstimulated whole saliva (UWS),gingival crevicular fluid (GCF) and serum of patients with chronic periodontitis before and after the initial phase of periodontal treatment and to determine whether they are possible diagnostic and prognostic biomarkers for periodontitis.Methods Twenty patients with periodontitis and 20 healthy controls were included

  6. Comparison of the pharmacokinetic profiles of two locally administered doxycycline gels in crevicular fluid and saliva.

    Science.gov (United States)

    Kim, Ti-Sun; Klimpel, Homa; Fiehn, Walter; Eickholz, Peter

    2004-04-01

    Controlled-release delivery systems enable the clinician to extend the half-life period of locally administered antibiotics in gingival crevicular fluid (GCF) significantly. The aim of this split-mouth study was to compare the pharmacokinetic profile of two different doxycycline gels (DOXY and ATRI) for topical subgingival application. Pharmacokinetics of both doxycycline gels were analyzed in GCF and saliva. In 10 patients suffering from severe periodontitis, 10 pairs of contralateral defects (pocket depth > or =5 mm/bleeding on probing or > or =6 mm) were randomly assigned either to the first application of DOXY or ATRI. Fourteen days after the topical application of the first antibiotic gel, the application of the second gel in the contralateral defect took place. Clinical examinations at baseline showed no significant differences between sites treated with DOXY and ATRI. Samples of GCF and saliva were drawn baseline, 2, 5 and 24 h after application, 2, 3, 4, 7, 9 and 11 days after application. Separation and quantitative measurement of both doxycycline-gels was performed with HPLC- and UV-detection at lambda=260 nm. In saliva specimens, time-dependent changes of mean doxycycline concentration were almost identical for both doxycycline-gels and declined from a maximum 2 h after application (ATRI: 6653.90+/-3096.14 microg/ml; DOXY: 5386.60+/-1542.02 microg/ml [arithmetic mean+/-SEM]) to zero values 9 days after application. In crevicular fluid specimens, sites treated with ATRI exhibited a faster decrease of mean doxycycline concentration (1085.30, 264.00, 273.94, and 258.00 microg/ml measured 2, 5, 24, and 48 h after application) than sites treated with DOXY (1388.38, 1300.40, 803.73, and 235.10 microg/ml). The faster decrease of ATRI compared with DOXY could not be proved to be statistically significantly different. Both doxycycline gels showed pharmacokinetics of controlled-release delivery systems.

  7. A comparative and observational assessment of gingival condition and il-1β level in vitiligo and nonvitiligo patients

    Directory of Open Access Journals (Sweden)

    Kharidhi Laxman Vandana

    2016-01-01

    Full Text Available Introduction: Vitiligo is an autoimmune skin disorder that causes loss of skin pigment. Loss of skin pigment in oral mucosa may also become prominent and tend to be more noticeable in people with darker skin. There is much less information concerning this condition in gingiva. Therefore, an initial attempt has been made to compare clinical gingival status and biochemical assessment in vitiligo and nonvitiligo patients. Materials and Methods: This observational pilot study includes 45 patients of age group 20–40 years were divided as nonvitiligo pigmented gingivitis as observational 1 group, nonvitiligo nonpigmented gingivitis as control group, and vitiligo with gingivitis as observational 2 group. The clinical parameters assessed were plaque index, gingival index (GI, gingival bleeding index (GBI, dummett oral pigmentation index, and biochemical parameter such as gingival crevicular fluid (GCF interleukin-1 beta (IL-1β levels. Results: On intergroup examination using unpaired t -test and statistical analysis, vitiligo group showed highly significant results in GI (P < 0.001 and GBI (P < 0.001 than nonvitiligo group. Furthermore, the GCF IL-1β levels were found to be lesser (34.16 ng/ml in vitiligo group as compared to nonvitiligo groups. Conclusion: At similar plaque level, higher gingival inflammation and bleeding were found in vitiligo patients. The loss of pigmentation of gingiva was evident in vitiligo patients. The possible defensive role of melanin is evident clinically.

  8. Expression of HMGB1 and HMGN2 in gingival tissues, GCF and PICF of periodontitis patients and peri-implantitis

    Directory of Open Access Journals (Sweden)

    Ping Xie

    2011-09-01

    Full Text Available High mobility group chromosomal protein B1 (HMGB1 and N2 (HMGN2, two members of High mobility group (HMG family, play important role in inflammation. The purposes of this study were to investigate the expression of HMGB1 and HMGN2 in periodontistis. The expression of HMGB1 and HMGN2 mRNA in gingival tissues and gingival crevicular fluid (GCF in chronic periodontitis (CP, generalized aggressive periodontitis (G-AgP patients and healthy subjects was detected by real-time PCR. The protein level of HMGB1 and HMGN2 in peri-implant crevicular fluid (PICF, peri-implant crevicular fluid of peri-implantitis (PI-PICF and normal patients was determined by Western blotting. Furthermore, IL-1β, IL-6, IL-8, TNF-α and HMGB1 levels in GCF, PI-PICF and healthy-PICF samples from different groups were determined by ELISA. HMGN2 expression was increased in inflamed gingival tissues and GCF from CP and G-ApG groups compared to control group. HMGB1 expression was the highest in the gingival tissues and GCF from CP patients and was accompanied by increased concentrations of IL-1β, IL-6, IL-8 proinflammaory cytokines. To our knowledge, this is the first study reporting that the expression of HMGB1 and HMGN2 was increased in the gingival tissues and GCF in CP and G-AgP and the PICF in PICF. Our data suggest that HMGB1 may be a potential target for the therapy of periodontitis and PI.

  9. Isolation and Multiple Differentiation Potential Assessment of Human Gingival Mesenchymal Stem Cells

    Directory of Open Access Journals (Sweden)

    Yuan Gao

    2014-11-01

    Full Text Available The aim of this study was to isolate human mesenchymal stem cells (MSCs from the gingiva (GMSCs and confirm their multiple differentiation potentials, including the odontogenic lineage. GMSCs, periodontal ligament stem cells (PDLSCs and dermal stem cells (DSCs cultures were analyzed for cell shape, cell cycle, colony-forming unit-fibroblast (CFU-F and stem cell markers. Cells were then induced for osteogenic and adipogenic differentiation and analyzed for differentiation markers (alkaline phosphatase (ALP activity, mineralization nodule formation and Runx2, ALP, osteocalcin (OCN and collagen I expressions for the osteogenic differentiation, and lipid vacuole formation and PPARγ-2 expression for the adipogenic differentiation. Besides, the odontogenic differentiation potential of GMSCs induced with embryonic tooth germ cell-conditioned medium (ETGC-CM was observed. GMSCs, PDLSCs and DSCs were all stromal origin. PDLSCs showed much higher osteogenic differentiation ability but lower adipogenic differentiation potential than DSCs. GMSCs showed the medial osteogenic and adipogenic differentiation potentials between those of PDLSCs and DSCs. GMSCs were capable of expressing the odontogenic genes after ETGC-CM induction. This study provides evidence that GMSCs can be used in tissue engineering/regeneration protocols as an approachable stem cell source.

  10. The phylum Synergistetes in gingivitis and necrotizing ulcerative gingivitis

    National Research Council Canada - National Science Library

    Baumgartner, Angelica; Thurnheer, Thomas; Lüthi-Schaller, Helga; Gmür, Rudolf; Belibasakis, Georgios N

    2012-01-01

    The clinical manifestation of necrotizing ulcerative gingivitis (NUG) is distinct from that of common gingivitis in that it is characterized by local necrosis of the gingival tissues, rapid onset, pain and extensive bleeding...

  11. Modulation of human dermal microvascular endothelial cell and human gingival fibroblast behavior by micropatterned silica coating surfaces for zirconia dental implant applications

    Science.gov (United States)

    Laranjeira, Marta S; Carvalho, Ângela; Pelaez-Vargas, Alejandro; Hansford, Derek; Ferraz, Maria Pia; Coimbra, Susana; Costa, Elísio; Santos-Silva, Alice; Fernandes, Maria Helena; Monteiro, Fernando Jorge

    2014-01-01

    Dental ceramic implants have shown superior esthetic behavior and the absence of induced allergic disorders when compared to titanium implants. Zirconia may become a potential candidate to be used as an alternative to titanium dental implants if surface modifications are introduced. In this work, bioactive micropatterned silica coatings were produced on zirconia substrates, using a combined methodology of sol–gel processing and soft lithography. The aim of the work was to compare the in vitro behavior of human gingival fibroblasts (HGFs) and human dermal microvascular endothelial cells (HDMECs) on three types of silica-coated zirconia surfaces: flat and micropatterned (with pillars and with parallel grooves). Our results showed that cells had a higher metabolic activity (HGF, HDMEC) and increased gene expression levels of fibroblast-specific protein-1 (FSP-1) and collagen type I (COL I) on surfaces with pillars. Nevertheless, parallel grooved surfaces were able to guide cell growth. Even capillary tube-like networks of HDMEC were oriented according to the surface geometry. Zirconia and silica with different topographies have shown to be blood compatible and silica coating reduced bacteria adhesion. All together, the results indicated that microstructured bioactive coating seems to be an efficient strategy to improve soft tissue integration on zirconia implants, protecting implants from peri-implant inflammation and improving long-term implant stabilization. This new approach of micropatterned silica coating on zirconia substrates can generate promising novel dental implants, with surfaces that provide physical cues to guide cells and enhance their behavior. PMID:27877662

  12. Modulation of human dermal microvascular endothelial cell and human gingival fibroblast behavior by micropatterned silica coating surfaces for zirconia dental implant applications

    Directory of Open Access Journals (Sweden)

    Marta S Laranjeira

    2014-03-01

    Full Text Available Dental ceramic implants have shown superior esthetic behavior and the absence of induced allergic disorders when compared to titanium implants. Zirconia may become a potential candidate to be used as an alternative to titanium dental implants if surface modifications are introduced. In this work, bioactive micropatterned silica coatings were produced on zirconia substrates, using a combined methodology of sol–gel processing and soft lithography. The aim of the work was to compare the in vitro behavior of human gingival fibroblasts (HGFs and human dermal microvascular endothelial cells (HDMECs on three types of silica-coated zirconia surfaces: flat and micropatterned (with pillars and with parallel grooves. Our results showed that cells had a higher metabolic activity (HGF, HDMEC and increased gene expression levels of fibroblast-specific protein-1 (FSP-1 and collagen type I (COL I on surfaces with pillars. Nevertheless, parallel grooved surfaces were able to guide cell growth. Even capillary tube-like networks of HDMEC were oriented according to the surface geometry. Zirconia and silica with different topographies have shown to be blood compatible and silica coating reduced bacteria adhesion. All together, the results indicated that microstructured bioactive coating seems to be an efficient strategy to improve soft tissue integration on zirconia implants, protecting implants from peri-implant inflammation and improving long-term implant stabilization. This new approach of micropatterned silica coating on zirconia substrates can generate promising novel dental implants, with surfaces that provide physical cues to guide cells and enhance their behavior.

  13. Modulation of human dermal microvascular endothelial cell and human gingival fibroblast behavior by micropatterned silica coating surfaces for zirconia dental implant applications

    Science.gov (United States)

    Laranjeira, Marta S.; Carvalho, Ângela; Pelaez-Vargas, Alejandro; Hansford, Derek; Ferraz, Maria Pia; Coimbra, Susana; Costa, Elísio; Santos-Silva, Alice; Fernandes, Maria Helena; Monteiro, Fernando Jorge

    2014-04-01

    Dental ceramic implants have shown superior esthetic behavior and the absence of induced allergic disorders when compared to titanium implants. Zirconia may become a potential candidate to be used as an alternative to titanium dental implants if surface modifications are introduced. In this work, bioactive micropatterned silica coatings were produced on zirconia substrates, using a combined methodology of sol-gel processing and soft lithography. The aim of the work was to compare the in vitro behavior of human gingival fibroblasts (HGFs) and human dermal microvascular endothelial cells (HDMECs) on three types of silica-coated zirconia surfaces: flat and micropatterned (with pillars and with parallel grooves). Our results showed that cells had a higher metabolic activity (HGF, HDMEC) and increased gene expression levels of fibroblast-specific protein-1 (FSP-1) and collagen type I (COL I) on surfaces with pillars. Nevertheless, parallel grooved surfaces were able to guide cell growth. Even capillary tube-like networks of HDMEC were oriented according to the surface geometry. Zirconia and silica with different topographies have shown to be blood compatible and silica coating reduced bacteria adhesion. All together, the results indicated that microstructured bioactive coating seems to be an efficient strategy to improve soft tissue integration on zirconia implants, protecting implants from peri-implant inflammation and improving long-term implant stabilization. This new approach of micropatterned silica coating on zirconia substrates can generate promising novel dental implants, with surfaces that provide physical cues to guide cells and enhance their behavior.

  14. Cytotoxicity of dental composite (co)monomers and the amalgam component Hg(2+) in human gingival fibroblasts.

    Science.gov (United States)

    Reichl, Franz-Xaver; Simon, Sabine; Esters, Magalie; Seiss, Mario; Kehe, Kai; Kleinsasser, Norbert; Hickel, Reinhard

    2006-08-01

    Unpolymerized resin (co)monomers or mercury (Hg) can be released from restorative dental materials (e.g. composites and amalgam). They can diffuse into the tooth pulp or the gingiva. They can also reach the gingiva and organs by the circulating blood after the uptake from swallowed saliva. The cytotoxicity of dental composite components hydroxyethylmethacrylate (HEMA), triethyleneglycoldimethacrylate (TEGDMA), urethanedimethacrylate (UDMA), and bisglycidylmethacrylate (Bis-GMA) as well as the amalgam component Hg(2+) (as HgCl(2)) and methyl mercury chloride (MeHgCl) was investigated on human gingival fibroblasts (HGFs) at two time intervals. To test the cytotoxicity of substances, the bromodeoxyuridine (BrdU) assay and the lactate dehydrogenase (LDH) assay were used. The test substances were added in various concentrations and cells were incubated for 24 or 48 h. The EC(50) values were obtained as half-maximum-effect concentrations from fitted curves. Following EC(50) values were found [BrdU: mean (mmol/l); SEM in parentheses; n=12]: (24 h/48 h) HEMA 8.860 (0.440)/6.600(0.630), TEGDMA 1.810(0.130)/1.220(0.130), UDMA 0.120(0.010)/0.140(0.010), BisGMA 0.060(0.004)/0.040(0.002), HgCl(2) 0.015(0.001)/0.050(0.006), and MeHgCl 0.004(0.001)/0.005(0.001). Following EC(50) values were found [LDH: mean (mmol/l); SEM in parentheses; n=12]: (24 h/48 h) HEMA 9.490(0.300)/7.890(1.230), TEGDMA 2.300(0.470)/1.950(0.310), UDMA 0.200(0.007)/0.100(0.007), BisGMA 0.070(0.005)/0.100(0.002), and MeHgCl 0.014(0.006)/0.010(0.003). In both assays, the following range of increased toxicity was found for composite components (24 and 48 h): HEMA < TEGDMA < UDMA < BisGMA. In both assays, MeHgCl was the most toxic substance. In the BrdU assay, Hg(2+) was about fourfold less toxic than MeHgCl but Hg(2+) was about fourfold more toxic than BisGMA. In the BrdU test, a significantly (P<0.05) decreased toxicity was observed for Hg(2+) at 48 h, compared to the 24 h Hg(2+)-exposure. A time depending

  15. Protein kinase A enhances lipopolysaccharide-induced IL-6, IL-8, and PGE2 production by human gingival fibroblasts

    Directory of Open Access Journals (Sweden)

    Ara Toshiaki

    2012-03-01

    Full Text Available Abstract Objective Periodontal disease is accompanied by inflammation of the gingiva and destruction of periodontal tissues, leading to alveolar bone loss in severe clinical cases. Interleukin (IL-6, IL-8, and the chemical mediator prostaglandin E2 (PGE2 are known to play important roles in inflammatory responses and tissue degradation. Recently, we reported that the protein kinase A (PKA inhibitor H-89 suppresses lipopolysaccharide (LPS-induced IL-8 production by human gingival fibroblasts (HGFs. In the present study, the relevance of the PKA activity and two PKA-activating drugs, aminophylline and adrenaline, to LPS-induced inflammatory cytokines (IL-6 and IL-8 and PGE2 by HGFs were examined. Methods HGFs were treated with LPS from Porphyromonas gingivalis and H-89, the cAMP analog dibutyryl cyclic AMP (dbcAMP, aminophylline, or adrenaline. After 24 h, IL-6, IL-8, and PGE2 levels were evaluated by ELISA. Results H-89 did not affect LPS-induced IL-6 production, but suppressed IL-8 and PGE2 production. In contrast, dbcAMP significantly increased LPS-induced IL-6, IL-8, and PGE2 production. Up to 10 μg/ml of aminophylline did not affect LPS-induced IL-6, IL-8, or PGE2 production, but they were significantly increased at 100 μg/ml. Similarly, 0.01 μg/ml of adrenaline did not affect LPS-induced IL-6, IL-8, or PGE2 production, but they were significantly increased at concentrations of 0.1 and 1 μg/ml. In the absence of LPS, H-89, dbcAMP, aminophylline, and adrenaline had no relevance to IL-6, IL-8, or PGE2 production. Conclusion These results suggest that the PKA pathway, and also PKA-activating drugs, enhance LPS-induced IL-6, IL-8, and PGE2 production by HGFs. However, aminophylline may not have an effect on the production of these molecules at concentrations used in clinical settings (8 to 20 μg/ml in serum. These results suggest that aminophylline does not affect inflammatory responses in periodontal disease.

  16. The significance and level detection of altered IL-17A and IL-35 in peri-implant gingival crevicular fluid%种植体周龈沟液IL-17 A和IL-35水平检测及意义

    Institute of Scientific and Technical Information of China (English)

    徐乙娜; 杨朝晖; 孔祥波; 伍虹

    2016-01-01

    Objective To analyze the similarities and differences of the expression of interleukin( IL)-17A and IL-35 between the group of peri-implantitis and peri-implant healthy patients, and to explore the mutual relationship and effect of IL-17A and IL-35 on the development and progress in peri-implantitis. Methods Peri-implant sulcular fluid( PISF) was collected from 20 subjects with healthy implants, and 20 with implantitis. The latter was made as comparison group after the implantitis was treated. The concentrations of IL-17A and IL-35 were determined by enzyme-linked immunosorbent assay method. Bleeding on probing(BOP), modified sulcus bleeding index( mSBI) and gingival index( GI) of the healthy implant group, the peri-implantitis group and the treated group were recorded and analyzed statistically. Results The concentrations of IL-17A and IL-35 in the peri-implantitis group were significantly higher than those in the healthy implant group (P<0. 05). IL-17A was slightly lower after treatment (P<0. 05), and IL-35 was slightly higher af-ter treatment (P<0. 05). The concentrations of IL-17A and IL-35 were negatively correlated in the peri-implantitis group. Conclusions The expressions of IL-35 and IL-17A are are closely related with the occurrence and development of peri-implantitis. IL-17A and IL-35 are potential molecular biological markers that may help assist diagnosis and treatment of implantitis.%目的:分析种植体组不同时期龈沟液(peri-implant sulcular fluid,PISF)中(interleukin,IL)-17A和IL-35水平改变,探讨其在种植体周围炎或黏膜炎发展过程中的作用和相互关系。方法收集2008—2013年在中山大学孙逸仙纪念医院口腔科行种植修复患者共40例为研究对象,分为种植体健康组20例和种植体炎症组20例,将种植体炎症组经过治疗后作为对照组。在种植体中有单冠修复、联冠修复。采集不同患者龈沟液并定量,对标本采用双抗体

  17. Idiopathic gingival fibromatosis

    National Research Council Canada - National Science Library

    Dani, Nitin Hemchandra; Khanna, Dinkar Parveen; Bhatt, Vaibhavi Hitesh; Joshi, Chaitanya Pradeep

    2015-01-01

    Idiopathic gingival fibromatosis (IGF) is a rare hereditary condition characterized by slowly progressive, nonhemorrhagic, fibrous enlargement of maxillary and mandibular keratinized gingiva caused by increase in submucosal connective...

  18. Identification of 1,8-cineole, borneol, camphor, and thujone as anti-inflammatory compounds in a Salvia officinalis L. infusion using human gingival fibroblasts.

    Science.gov (United States)

    Ehrnhöfer-Ressler, Miriam M; Fricke, Kristina; Pignitter, Marc; Walker, Joel M; Walker, Jessica; Rychlik, Michael; Somoza, Veronika

    2013-04-10

    Drinking or gargling Salvia officinalis L. infusion (sage infusion) is thought to soothe a sore throat, tonsillitis, and inflamed, red gums, although structure-based scientific evidence for the key anti-inflammatory compounds in sage infusion is scarce. Human gingival fibroblasts (HGF-1) were treated with sage infusion (SI) or SI fractions containing either its volatile components and water (aqueous distillate, AD) or its dry matter (DM) for six hours. SI, AD, and DM reduced a mean phorbol-12-myristate-13-acetate/ionomycin (PMA/I)-stimulated release of the pro-inflammatory interleukins IL-6 and IL-8 by more than 50% (p < 0.05). Cellular uptake experiments and subsequent GC-MS analysis using stable-isotope-labeled internal standards revealed the presence of 1,8-cineole, borneol, camphor, and α-/β-thujone in SI-treated cells; LC-MS analysis demonstrated the presence of rosmarinic acid. A significant, more than 50% mean inhibition of PMA/I-induced IL-6 and IL-8 release was demonstrated for the volatile compounds 1,8-cineole, borneol, camphor, and thujone, but not for the nonvolatile rosmarinic acid when applied in concentrations representative of sage infusion. Therefore, the volatile compounds were found to be more effective than rosmarinic acid. 1,8-Cineole, borneol, camphor, and α-/β-thujone chiefly contribute to the anti-inflammatory activity of sage infusion in human gingival fibroblasts.

  19. Comparative systems toxicology analysis of cigarette smoke and aerosol from a candidate modified risk tobacco product in organotypic human gingival epithelial cultures: A 3-day repeated exposure study.

    Science.gov (United States)

    Zanetti, Filippo; Titz, Bjoern; Sewer, Alain; Lo Sasso, Giuseppe; Scotti, Elena; Schlage, Walter K; Mathis, Carole; Leroy, Patrice; Majeed, Shoaib; Torres, Laura Ortega; Keppler, Brian R; Elamin, Ashraf; Trivedi, Keyur; Guedj, Emmanuel; Martin, Florian; Frentzel, Stefan; Ivanov, Nikolai V; Peitsch, Manuel C; Hoeng, Julia

    2017-03-01

    Smoking is one of the major lifestyle-related risk factors for periodontal diseases. Modified risk tobacco products (MRTP) offer a promising alternative in the harm reduction strategy for adult smokers unable to quit. Using a systems toxicology approach, we investigated and compared the exposure effects of a reference cigarette (3R4F) and a heat-not-burn technology-based candidate MRTP, the Tobacco Heating System (THS) 2.2. Human gingival epithelial organotypic cultures were repeatedly exposed (3 days) for 28 min at two matching concentrations of cigarette smoke (CS) or THS2.2 aerosol. Results showed only minor histopathological alterations and minimal cytotoxicity upon THS2.2 aerosol exposure compared to CS (1% for THS2.2 aerosol vs. 30% for CS, at the high concentration). Among the 14 proinflammatory mediators analyzed, only 5 exhibited significant alterations with THS2.2 exposure compared with 11 upon CS exposure. Transcriptomic and metabolomic analysis indicated a general reduction of the impact in THS2.2 aerosol-exposed samples with respect to CS (∼79% lower biological impact for the high THS2.2 aerosol concentration compared to CS, and 13 metabolites significantly perturbed for THS2.2 vs. 181 for CS). This study indicates that exposure to THS2.2 aerosol had a lower impact on the pathophysiology of human gingival organotypic cultures than CS. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

  20. Cytotoxicity of dental composite (co)monomers and the amalgam component Hg{sup 2+} in human gingival fibroblasts

    Energy Technology Data Exchange (ETDEWEB)

    Reichl, Franz-Xaver; Simon, Sabine; Esters, Magalie; Seiss, Mario [Ludwig-Maximilians-University of Munich, Walther-Straub-Institute of Pharmacology and Toxicology, Munich (Germany); Kehe, Kai [Bundeswehr Institute of Pharmacology and Toxicology, Munich (Germany); Kleinsasser, Norbert [University of Regensburg, Department of Otolaryngology - Head and Neck Surgery, Regensburg (Germany); Hickel, Reinhard [Ludwig-Maximilians-University, Department of Operative Dentistry and Periodontology, Munich (Germany)

    2006-08-15

    Unpolymerized resin (co)monomers or mercury (Hg) can be released from restorative dental materials (e.g. composites and amalgam). They can diffuse into the tooth pulp or the gingiva. They can also reach the gingiva and organs by the circulating blood after the uptake from swallowed saliva. The cytotoxicity of dental composite components hydroxyethylmethacrylate (HEMA), triethyleneglycoldimethacrylate (TEGDMA), urethanedimethacrylate (UDMA), and bisglycidylmethacrylate (Bis-GMA) as well as the amalgam component Hg{sup 2+} (as HgCl{sub 2}) and methyl mercury chloride (MeHgCl) was investigated on human gingival fibroblasts (HGFs) at two time intervals. To test the cytotoxicity of substances, the bromodeoxyuridine (BrdU) assay and the lactate dehydrogenase (LDH) assay were used. The test substances were added in various concentrations and cells were incubated for 24 or 48 h. The EC{sub 50} values were obtained as half-maximum-effect concentrations from fitted curves. Following EC{sub 50} values were found [BrdU: mean (mmol/l); SEM in parentheses; n=12]: (24 h/48 h) HEMA 8.860 (0.440)/6.600(0.630), TEGDMA 1.810(0.130)/1.220(0.130), UDMA 0.120(0.010)/0.140(0.010), BisGMA 0.060(0.004)/0.040(0.002), HgCl{sub 2} 0.015(0.001)/0.050(0.006), and MeHgCl 0.004(0.001)/0.005(0.001). Following EC{sub 50} values were found [LDH: mean (mmol/l); SEM in parentheses; n=12]: (24 h/48 h) HEMA 9.490(0.300)/7.890(1.230), TEGDMA 2.300(0.470)/1.950(0.310), UDMA 0.200(0.007)/0.100(0.007), BisGMA 0.070(0.005)/0.100(0.002), and MeHgCl 0.014(0.006)/0.010(0.003). In both assays, the following range of increased toxicity was found for composite components (24 and 48 h): HEMA < TEGDMA < UDMA < BisGMA. In both assays, MeHgCl was the most toxic substance. In the BrdU assay, Hg{sup 2+} was about fourfold less toxic than MeHgCl but Hg{sup 2+} was about fourfold more toxic than BisGMA. In the BrdU test, a significantly (P<0.05) decreased toxicity was observed for Hg{sup 2+} at 48 h, compared to the 24 h

  1. 人特异性蛋白激酶-15基因在增生牙龈组织中表达的研究%The expression of human-specific protein kinase-15 in the hyperplastic gingival tissues

    Institute of Scientific and Technical Information of China (English)

    梅幼敏; 宦泓; 周艳; 谢海燕; 张汝阳; 徐艳

    2012-01-01

    Objective:To detect the human-specific protein kinase-15(NYD-SP15)gene expression in normal gingival tissue and hyperplastic gingival tissues,observe the expression of NYD-SP15 protein in gingival tissues,and to explore the relationship of NYD- SP15 and gingival hyperplasia initially. Methods:Reverse transcription-polymerase chain reaction(RT-PCR)and immunohistochemical method were applicated to detect the expression of NYD-SP15 gene in gingival tissues of 27 cases of normal people and 40 cases of gingival hyperplasia patients. The normal gingival tissues were the excision of gingival tissue during crown lengthening surgery(the teeth were traumatic crown fractures),recorded as normal control group. The hyperplasia samples were the excision of gingival tissue during gingivectomy, recorded as hyperplasia group. The half of all specimens were removed and stored in nitrogen liquid immediately. The remaining half of the tissues with formalin, embedded in paraffin storage. Staining positive cells were counted under the microscope and classificated by positive degree. The data of the experiment was analyzed by Stata 9.2(StataCorporation College Station,TX)software. Results:The expression of NYD-SP15 mRNA in tissues of hyperplasia group was significantly higher than that of control group(P < 0.05). The immunohistochemistry results showed that the NYD-SP15 protein was highly expression in the cytoplasm in normal gingival tissue. But,in the hyplasia gingival tissues,the expression of NYD-SP15 protein was highly in the nucleus. Conclusion;The level of NYD-SP15 expression and location in the gingival tissues may be associated with the gingival hyperplasia. However,the relationship between them remains to be further studied.%目的:检测细胞增殖相关基因人特异性蛋白激酶-15(specific protein kinase 15,NYD-SP15)基因在增生牙龈及正常牙龈组织中的表达,初步探讨该基因与牙龈增生发生的关系.方法:应用RT-PCR及免疫组化法检测27例

  2. Effect of Periodontal Therapy on Crevicular Fluid Interleukin-6 and Interleukin-8 Levels in Chronic Periodontitis

    Directory of Open Access Journals (Sweden)

    Paschalina Goutoudi

    2012-01-01

    Full Text Available Purpose. The aim of this study was to analyse the levels of interleukin-6 (IL-6 and interleukin-8 (IL-8 in gingival crevicular fluid (GCF of patients with chronic periodontitis prior to and following surgical and/or nonsurgical periodontal therapy for a period of 32 weeks. Methods. GCF samples were obtained from 24 nondiseased and 72 diseased sites of 12 periodontal patients prior to as well as at 6, 16, and 32 weeks following non-surgical and surgical periodontal therapy. IL-6 and IL-8 levels were determined by enzyme-linked immunosorbent assay (ELISA. Results. Periodontal treatment improved all clinical parameters. Both treatment modalities resulted in similar IL-6 as well as IL-8 levels. Mean IL-6 and IL-8 concentrations were significantly higher in non-diseased compared to diseased sites and increased significantly following treatment in diseased sites. Mean total amounts of IL-6 and IL-8 (TAIL-6, TAIL-8 did not differ significantly between diseased and nondiseased sites, while following therapy TAIL-8 levels decreased significantly. Conclusions. The data suggest that periodontal therapy reduced the levels of IL-8 in GCF. However, a strong relationship between IL-6, IL-8 amounts in GCF and periodontal destruction and inflammation was not found.

  3. Evaluation of Metalloproteinase-8 Levels in Crevicular Fluid of Patients with Healthy Implants or Periodontitis

    Directory of Open Access Journals (Sweden)

    Paweł Aleksandrowicz

    2017-01-01

    Full Text Available Evaluation of periodontal and peri-implant tissue condition is mainly based on clinical examination and imaging diagnostics. Some data imply that Metalloproteinase-8 (MMP-8 level examination in peri-implant sulcular fluid (PISF might be useful for evaluating the condition of peri-implant tissues and monitoring a development of peri-implant inflammation, including both mucositis and peri-implantitis. Hence, in this study, we decided to evaluate the level of MMP-8 in PISF obtained from patients without clinical symptoms of mucositis or peri-implantitis and compare it with MMP-8 level in gingival crevicular fluid (GCF obtained from patients with healthy periodontium and those with varying severity of periodontitis. A total of 189 subjects were included in the study, and GCF/PISF samples were analysed for MMP-8 level by ELISA test. We documented that MMP-8 level in PISF obtained from patients without symptoms of mucositis or peri-implantitis was significantly higher not only than in GCF of periodontally healthy patients but also, which seems to be very interesting, than in GCF of patients with varying degrees of periodontal inflammation, consistent with earlier studies. Our observation might imply that monitoring of MMP-8 level in PISF could help to diagnose mucositis/peri-implantitis in an early stage, prior to clinical manifestations, which may allow for quick start of appropriate therapy.

  4. Chemerin as a Novel Crevicular Fluid Marker of Patients With Periodontitis and Type 2 Diabetes Mellitus.

    Science.gov (United States)

    Doğan, Şeyma Bozkurt; Ballı, Umut; Dede, Figen Öngöz; Sertoğlu, Erdim; Tazegül, Kaan

    2016-08-01

    The objectives of the present study are to: 1) determine whether gingival crevicular fluid (GCF) chemerin is a novel predictive marker for patients with chronic periodontitis (CP) with and without type 2 diabetes mellitus (t2DM); 2) analyze the relationship between chemerin and interleukin (IL)-6 in periodontally healthy individuals and in patients with CP and with and without t2DM; and 3) evaluate the effect of non-surgical periodontal therapy on GCF chemerin levels. Eighty individuals were split into four groups: 20 who were systemically and periodontally healthy (CTRL), 20 with t2DM and periodontally healthy (DM-CTRL), 20 systemically healthy with CP (CP), and 20 with CP and t2DM (DM-CP). Individuals with periodontitis were treated with non-surgical periodontal therapy. GCF sampling procedures and clinical periodontal measures were performed before and 6 weeks after treatment. Enzyme-linked immunosorbent assay was used to measure chemerin and IL-6 levels. Greater values for GCF chemerin and IL-6 levels were found in CP groups than in periodontally healthy groups, in DM-CP than in CP, and in DM-CTRL than in CTRL (P periodontitis and t2DM induced aberrant secretion of chemerin, and non-surgical periodontal therapy influenced the decrease of GCF chemerin levels in patients with CP with and without t2DM. Furthermore, it suggests GCF chemerin levels may be considered a potential proinflammatory marker for diabetes, periodontal disease, and treatment outcomes.

  5. Idiopathic gingival fibromatosis

    Directory of Open Access Journals (Sweden)

    Nitin Hemchandra Dani

    2015-01-01

    Full Text Available Idiopathic gingival fibromatosis (IGF is a rare hereditary condition characterized by slowly progressive, nonhemorrhagic, fibrous enlargement of maxillary and mandibular keratinized gingiva caused by increase in submucosal connective tissue elements, mostly associated with some syndrome. This case report describes a case of nonsyndromic generalized IGF in an 18-year-old male patient who presented with generalized gingival enlargement. The enlarged tissue was surgically removed by internal bevel gingivectomy and ledge and wedge procedure. The patient was regularly monitored clinically for improvement in his periodontal condition as well as for any recurrence of gingival overgrowth.

  6. Idiopathic gingival fibromatosis

    Science.gov (United States)

    Dani, Nitin Hemchandra; Khanna, Dinkar Parveen; Bhatt, Vaibhavi Hitesh; Joshi, Chaitanya Pradeep

    2015-01-01

    Idiopathic gingival fibromatosis (IGF) is a rare hereditary condition characterized by slowly progressive, nonhemorrhagic, fibrous enlargement of maxillary and mandibular keratinized gingiva caused by increase in submucosal connective tissue elements, mostly associated with some syndrome. This case report describes a case of nonsyndromic generalized IGF in an 18-year-old male patient who presented with generalized gingival enlargement. The enlarged tissue was surgically removed by internal bevel gingivectomy and ledge and wedge procedure. The patient was regularly monitored clinically for improvement in his periodontal condition as well as for any recurrence of gingival overgrowth. PMID:26941525

  7. Expression of Human β-defensin-2 in Gingival Epithelial Cell%牙龈上皮细胞中人β2——防御素mRNA的表达

    Institute of Scientific and Technical Information of China (English)

    魏洪涛; 罗云纲; 王国珍; 李美华

    2013-01-01

    Objective:To examine the expression of human β-defensin-2(hBD-2) in gingival epithelial cell of normal subjects and patients with gingival hyperplasia,and to investigate the role of β-defensin 2 in the innate immunity.Methods:Gingival tissues were obtained from 10 normal subjects and from 12 patients undergoing surgery for gingival hyperplasia.Isolated epithelial cells were used for total RNA isolation by reverse transcriptase-polymerase chain reaction RT-PCR).Results:β-defensin-2 mRNA was detected in the gingival epithelium of patients with gingival hyperplasia normal gingival tissue.Its expression level was significantly higher in diseased epithelium than in normal turbinate epithelium (P<0.05).Conclusion:These results suggest that beta-defensin 2 may play a constitutive role in gingival defenses.It may be induced in response to local infection or inflammation.%目的:检测人β防御素(human β defensin,hBD)2 mRNA在牙龈增生病人和正常人的牙龈上皮细胞中的表达,分析hBD=2mRNA在牙龈组织天然免疫中的作用.方法:应用逆转录一聚合酶链反应技术(reverse transcriptase-polymerase chain reaction,RT-PCR)检测12例牙龈增生病人和10例正常牙龈上皮细胞中hBD-2mRNA的表达.结果:hBD-2 mRNA在12例牙龈增生和10例正常对照牙龈上皮细胞中均有表达,且两组间差异有统计学意义(P<0.05).结论:牙龈上皮细胞中hBD-2在天然免疫中发挥重要作用,hBD-2的表达增加可能与牙龈增生中的细胞因子有关.

  8. Doxycycline inhibits neutrophil (PMN)-type matrix metalloproteinases in human adult periodontitis gingiva.

    Science.gov (United States)

    Golub, L M; Sorsa, T; Lee, H M; Ciancio, S; Sorbi, D; Ramamurthy, N S; Gruber, B; Salo, T; Konttinen, Y T

    1995-02-01

    We previously reported that low-dose doxycycline (DOXY) therapy reduces host-derived collagenase activity in gingival tissue of adult periodontitis (AP) patients. However, it was not clear whether this in vivo effect was direct or indirect. In the present study, inflamed human gingival tissue, obtained from AP patients during periodontal surgery, was extracted and the extracts partially purified by (NH4)2SO4 precipitation. The extracts were then analyzed for collagenase activity using SDS-PAGE/fluorography/laser densitometry, and for gelatinase activity using type I gelatin zymography as well as a new quantitative assay using biotinylated type I gelatin as substrate. DOXY was added to the incubation mixture at a final concentration of 0-1000 microM. The concentration of DOXY required to inhibit 50% of the gingival tissue collagenase (IC50) was found to be 16-18 microM in the presence or absence of 1.2 mM APMA (an optimal organomercurial activator of latent procollagenases); this IC50 for DOXY was similar to that exhibited for collagenase or matrix metalloproteinase (MMP)-8 from polymorphonuclear leukocytes (PMNs) and from gingival crevicular fluid (GCF) of AP patients. Of interest, Porphyromonas gingivalis collagenase was also inhibited by similar DOXY levels (IC50 = 15 microM), however the collagenase activity observed in the gingival tissue extracts was found to be of mammalian not bacterial origin based on the production of the specific alpha A (3/4) and alpha B (1/4) collagen degradation fragments. In contrast, the inhibition of collagenase purified from culture media of human gingival fibroblasts (MMP-1) required much greater DOXY levels (IC50 = 280 microM). The predominant molecular forms of gelatinolytic activity presented in the AP patients gingival tissue extracts were found to closely correspond to the 92 kD PMN-type gelatinase (MMP-9) although small quantities of 72 kD fibroblast-type gelatinase (MMP-2), and some other low molecular weight gelatinases

  9. Association between gingival crevicular fluid prostaglandin E 2 level and preterm low birth weight

    Directory of Open Access Journals (Sweden)

    Fouzia Tarannum

    2012-01-01

    Conclusion: The study provides weak evidence that there is correlation between GCF-PGE 2 levels and birth outcome. Further clinical trials with large samples are required to confirm the association between GCF-PGE 2 levels and PLBW.

  10. Matrix metalloproteinases and tissue inhibitors of metalloproteinases in gingival crevicular fluid during orthodontic tooth movement

    NARCIS (Netherlands)

    Bildt, Miriam; Bloemen, M; Kuijpers-Jagtman, A.M.; Von Den Hoff, Johannes W

    2009-01-01

    Orthodontic tooth movement requires extensive re-modelling of the periodontium. Matrix metalloproteinases (MMPs) degrade the extracellular matrix during re-modelling, while their activity is regulated by the tissue inhibitors of metalloproteinases (TIMPs). The aim of this study was to investigate di

  11. Hepatocyte Growth Factor Levels in the Saliva and Gingival Crevicular Fluid in Smokers with Periodontitis

    OpenAIRE

    Sukumaran Anil; Sajith Vellappally; Preethanath, R. S.; Sameer A. Mokeem; Hani S. AlMoharib; Shankargouda Patil; Elna P. Chalisserry; Al Kheraif, Abdulaziz A.

    2014-01-01

    Hepatocyte growth factor (HGF) production by oral fibroblasts is enhanced by various molecules that are induced during inflammatory conditions including periodontitis. HGF plays an important role in the progression of periodontitis, by stimulating intense growth of epithelial cells and preventing regeneration of connective tissue attachments. Smokers have a greater risk factor in the pathogenesis and progression of periodontal disease. The objective of the study was to estimate the level of H...

  12. Matrix metalloproteinases and tissue inhibitors of metalloproteinases in gingival crevicular fluid during orthodontic tooth movement

    NARCIS (Netherlands)

    Bildt, Miriam; Bloemen, M; Kuijpers-Jagtman, A.M.; Von Den Hoff, Johannes W

    2009-01-01

    Orthodontic tooth movement requires extensive re-modelling of the periodontium. Matrix metalloproteinases (MMPs) degrade the extracellular matrix during re-modelling, while their activity is regulated by the tissue inhibitors of metalloproteinases (TIMPs). The aim of this study was to investigate

  13. Evaluation of gingival crevicular fluid transforming growth factor-β1 ...

    African Journals Online (AJOL)

    2015-09-11

    Sep 11, 2015 ... growth factor-β1 level after treatment of intrabony periodontal ... the treatment of intrabony periodontal defects of chronic periodontitis ...... fluid of periodontitis patients with and without type 2 diabetes: A literature review.

  14. Hereditary Gingival Fibromatosis

    Science.gov (United States)

    Nevin, N. C.

    1971-01-01

    Case studies of two siblings suffering from a gum disorder in which enlargement of the gingival mucosa is caused by a fibrosis. The disorder in the two children was felt to be an hereditary recessive trait. (CD)

  15. The effects of enamel matrix derivative and cyclic mechanical strain on human gingival fibroblasts in an in vitro defect healing model.

    Science.gov (United States)

    Sanders, Jill E; Chuang, Augustine; Swiec, Gary D; Bisch, Fredrick C; Herold, Robert W; Buxton, Thomas B; McPherson, James C

    2011-01-01

    Gingival fibroblasts (GFs) play a considerable role in the maintenance of the gingival apparatus as well as in connective tissue repair. Mobility of a periodontal wound or soft tissue graft can impair connective tissue healing from the GFs. Enamel matrix derivative (EMD) is an enamel matrix protein used clinically for periodontal regeneration of intrabony defects and furcations, as well as treatment of gingival margin recessions. The goal of this project was to compare the effects of varying concentrations of EMD, with and without cyclic mechanical strain, on cellular wound fill of human GFs using an in vitro defect healing model. GFs were seeded and cultured in six-well flexible-bottomed plates. A 3-mm wound was created in the central portion of each confluent well. Three wells were treated with each EMD concentration of 0 Μg/mL (control), 30 Μg/mL, 60 Μg/mL, or 120 Μg/mL. The plates were placed in an incubator containing a strain unit to subject test plates to cyclic strain. An identical set of control plates were not flexed. Cells were examined on days 4, 8, 12, and 16. Microphotographs were taken and wound fill measurements made using image analysis software. The percent wound fill was calculated. All nonflexed plates, regardless of EMD concentration, reached > 90% defect fill at similar rates by day 16. However, in the flexed plates, EMD had a significant negative effect on defect fill. The defect fill was 55.7% for 0 Μg/mL EMD, 48.2% for 30 Μg/mL EMD, 36.7% for 60 Μg/mL EMD, and 34.1% for 120 Μg/mL EMD on day 16 for the flexed GFs. EMD, in concentrations as high as 120 Μg/mL, did not significantly affect the amount of defect fill with nonflexed GFs. However, when the GFs were flexed, the addition of EMD had a significant negative effect on defect fill in a dose-dependent manner.

  16. Gingival Cyst of Newborn.

    Science.gov (United States)

    Moda, Aman

    2011-01-01

    Gingival cyst of newborn is an oral mucosal lesion of transient nature. Although it is very common lesion within 3 to 6 weeks of birth, it is very rare to visualize the lesion thereafter. Presented here is a case report of gingival cyst, which was visible just after 15 days of birth. Clinical diagnoses of these conditions are important in order to avoid unnecessary therapeutic procedure and provide suitable information to parents about the nature of the lesion.

  17. Peri-Implant Crevicular Fluid Analysis, Enzymes and Biomarkers: a Systemetic Review

    Directory of Open Access Journals (Sweden)

    Erhan Dursun

    2016-09-01

    Full Text Available Objectives: To review the current understanding of the biomarkers and enzymes associated with different forms peri-implant diseases and how their level changes influence the pathogenesis of the inflammatory diseases around dental implants. Material and Methods: An electronic search in two different databases was performed including MEDLINE (PubMed and EMBASE between 1996 to 2016. Human studies analyse peri-implant crevicular fluid (PICF biomarker and enzyme levels of implants having peri-implant mucositis and peri-implantitis published in English language, were evaluated. A systematic review was performed to assess which biomarkers and enzymes in PICF were used to identify the inflammatory conditions around dental implants. Results: Fifty-one articles were identified of which 41 were further evaluated and included in the analysis. Due to significant heterogeneity between included studies, a meta-analysis could not be performed. Instead, a systematic descriptive review was performed. Conclusions: Biomarkers and enzymes in peri-implant crevicular fluid have shown promising results in differentiating from peri-implant disease condition to health. However, due to inconsistent results and acquiring much evidence from cross-sectional studies, additional evidence supported by randomized-controlled trials is needed to validate the links reported.

  18. Increased Eotaxin and MCP-1 Levels in Serum from Individuals with Periodontitis and in Human Gingival Fibroblasts Exposed to Pro-Inflammatory Cytokines

    Science.gov (United States)

    Sulniute, Rima; Palmqvist, Py; Majster, Mirjam; Holm, Cecilia Koskinen; Zwicker, Stephanie; Clark, Reuben; Önell, Sebastian; Johansson, Ingegerd; Lerner, Ulf H.; Lundberg, Pernilla

    2015-01-01

    Periodontitis is a chronic inflammatory disease of tooth supporting tissues resulting in periodontal tissue destruction, which may ultimately lead to tooth loss. The disease is characterized by continuous leukocyte infiltration, likely mediated by local chemokine production but the pathogenic mechanisms are not fully elucidated. There are no reliable serologic biomarkers for the diagnosis of periodontitis, which is today based solely on the degree of local tissue destruction, and there is no available biological treatment tool. Prompted by the increasing interest in periodontitis and systemic inflammatory mediators we mapped serum cytokine and chemokine levels from periodontitis subjects and healthy controls. We used multivariate partial least squares (PLS) modeling and identified monocyte chemoattractant protein-1 (MCP-1) and eotaxin as clearly associated with periodontitis along with C-reactive protein (CRP), years of smoking and age, whereas the number of remaining teeth was associated with being healthy. Moreover, body mass index correlated significantly with serum MCP-1 and CRP, but not with eotaxin. We detected higher MCP-1 protein levels in inflamed gingival connective tissue compared to healthy but the eotaxin levels were undetectable. Primary human gingival fibroblasts displayed strongly increased expression of MCP-1 and eotaxin mRNA and protein when challenged with tumor necrosis factor-α (TNF-α and interleukin-1β (IL-1β), key mediators of periodontal inflammation. We also demonstrated that the upregulated chemokine expression was dependent on the NF-κΒ pathway. In summary, we identify higher levels of CRP, eotaxin and MCP-1 in serum of periodontitis patients. This, together with our finding that both CRP and MCP-1 correlates with BMI points towards an increased systemic inflammatory load in patients with periodontitis and high BMI. Targeting eotaxin and MCP-1 in periodontitis may result in reduced leukocyte infiltration and inflammation in

  19. Pitanga (Eugenia uniflora L.) fruit juice and two major constituents thereof exhibit anti-inflammatory properties in human gingival and oral gum epithelial cells.

    Science.gov (United States)

    Josino Soares, Denise; Walker, Jessica; Pignitter, Marc; Walker, Joel Michael; Imboeck, Julia Maria; Ehrnhoefer-Ressler, Miriam Margit; Montenegro Brasil, Isabella; Somoza, Veronika

    2014-11-01

    Pitanga, Eugenia uniflora L., is a tropical fruit, which may be consumed as juice. While beneficial health effects of Eugenia uniflora L. leaf extracts have extensively been studied, limited data are available on an anti-inflammatory potential of pitanga juice. The aim of the presented study was to investigate anti-inflammatory properties of pitanga juice with regards to a prevention of inflammation-related periodontal diseases. For this purpose, six healthy volunteers swirled pitanga juice, containing 35% pitanga pulp, for 10 min. Thereafter, oral gum epithelial cells were harvested using a sterile brush and stimulated with lipopolysaccharides from Porphyromonas gingivalis (PG-LPS) for 6 h. Furthermore, human gingival fibroblasts (HGF-1) were used to elucidate the anti-inflammatory potential of pitanga juice constituents, cyanidin-3-glucoside and oxidoselina-1,3,7(11)-trien-8-one, in juice representative concentrations of 119 μg ml(-1) and 30 μg ml(-1), respectively. For the first time, an anti-inflammatory impact of pitanga juice on gingival epithelial cells was shown by means of an attenuation of IL-8 release by 55 ± 8.2% and 52 ± 11% in non-stimulated and PG-LPS-stimulated cells, respectively. In addition, both cyanidin-3-glucoside and oxidoselina-1,3,7(11)-trien-8-one reduced the LPS-stimulated CXCL8 mRNA expression by 50 ± 15% and 37 ± 18% and IL-8 release by 52 ± 9.9% and 45 ± 3.7% in HGF-1 cells, when concomitantly incubated with 10 μg ml(-1)PG-LPS for 6 h, revealing an anti-inflammatory potential of the volatile compound oxidoselina-1,3,7(11)-trien-8-one for the first time.

  20. Increased Eotaxin and MCP-1 Levels in Serum from Individuals with Periodontitis and in Human Gingival Fibroblasts Exposed to Pro-Inflammatory Cytokines.

    Science.gov (United States)

    Boström, Elisabeth A; Kindstedt, Elin; Sulniute, Rima; Palmqvist, Py; Majster, Mirjam; Holm, Cecilia Koskinen; Zwicker, Stephanie; Clark, Reuben; Önell, Sebastian; Johansson, Ingegerd; Lerner, Ulf H; Lundberg, Pernilla

    2015-01-01

    Periodontitis is a chronic inflammatory disease of tooth supporting tissues resulting in periodontal tissue destruction, which may ultimately lead to tooth loss. The disease is characterized by continuous leukocyte infiltration, likely mediated by local chemokine production but the pathogenic mechanisms are not fully elucidated. There are no reliable serologic biomarkers for the diagnosis of periodontitis, which is today based solely on the degree of local tissue destruction, and there is no available biological treatment tool. Prompted by the increasing interest in periodontitis and systemic inflammatory mediators we mapped serum cytokine and chemokine levels from periodontitis subjects and healthy controls. We used multivariate partial least squares (PLS) modeling and identified monocyte chemoattractant protein-1 (MCP-1) and eotaxin as clearly associated with periodontitis along with C-reactive protein (CRP), years of smoking and age, whereas the number of remaining teeth was associated with being healthy. Moreover, body mass index correlated significantly with serum MCP-1 and CRP, but not with eotaxin. We detected higher MCP-1 protein levels in inflamed gingival connective tissue compared to healthy but the eotaxin levels were undetectable. Primary human gingival fibroblasts displayed strongly increased expression of MCP-1 and eotaxin mRNA and protein when challenged with tumor necrosis factor-α (TNF-α and interleukin-1β (IL-1β), key mediators of periodontal inflammation. We also demonstrated that the upregulated chemokine expression was dependent on the NF-κΒ pathway. In summary, we identify higher levels of CRP, eotaxin and MCP-1 in serum of periodontitis patients. This, together with our finding that both CRP and MCP-1 correlates with BMI points towards an increased systemic inflammatory load in patients with periodontitis and high BMI. Targeting eotaxin and MCP-1 in periodontitis may result in reduced leukocyte infiltration and inflammation in

  1. microRNA-142 is upregulated by tumor necrosis factor-alpha and triggers apoptosis in human gingival epithelial cells by repressing BACH2 expression

    Science.gov (United States)

    Li, Song; Song, Zhongchen; Dong, Jiachen; Shu, Rong

    2017-01-01

    Tumor necrosis factor-alpha (TNF-α) has been shown to cause apoptosis of gingival epithelial cells (GECs) in periodontitis. However, the underlying molecular mechanism is still unclear. In this study, we showed that miR-142 expression was significantly elevated in human GECs after exposure to TNF-α. Such induction was in a time- and concentration-dependent manner. Serum miR-142 levels were positively correlated with serum TNF-α levels in patients with chronic periodontitis (r = 0.314, P = 0.0152). Depletion of miR-142 was found to attenuate TNF-α-induced apoptosis, as determined by TUNEL staining and caspase-3 activity assays. In contrast, overexpression of miR-142 significantly reduced viability and induced apoptosis in GECs. Basic leucine zipper transcription factor 2 (BACH2) was identified to be a functional target of miR-142. Overexpression of miR-142 caused a 3-fold reduction of BACH2 protein in primary GECs. Overexpression of BACH2 significantly reversed miR-142- or TNF-α-induced apoptosis of GECs. Similar to the findings with miR-142 mimic, depletion of BACH2 significantly promoted apoptosis in GECs, which was accompanied by decreased expression of Bcl-2 and Bcl-xL and increased expression of Bax and Bim. Overall, miR-142 mediates TNF-α-induced apoptosis in gingival epithelial cells by targeting BACH2 and may represent a potential therapeutic target for periodontitis. PMID:28123644

  2. Cytotoxicity of HBD3 for dendritic cells, normal human epidermal keratinocytes, hTERT keratinocytes, and primary oral gingival epithelial keratinocytes in cell culture conditions.

    Science.gov (United States)

    Leelakanok, Nattawut; Fischer, Carol L; Bates, Amber M; Guthmiller, Janet M; Johnson, Georgia K; Salem, Aliasger K; Brogden, Kim A; Brogden, Nicole K

    2015-12-03

    Human β-defensin 3 (HBD3) is a prominent host defense peptide. In our recent work, we observed that HBD3 modulates pro-inflammatory agonist-induced chemokine and cytokine responses in human myeloid dendritic cells (DCs), often at 20.0 μM concentrations. Since HBD3 can be cytotoxic in some circumstances, it is necessary to assess its cytotoxicity for DCs, normal human epidermal keratinocytes (NHEKs), human telomerase reverse transcriptase (hTERT) keratinocytes, and primary oral gingival epithelial (GE) keratinocytes in different cell culture conditions. Cells, in serum free media with resazurin and in complete media with 10% fetal bovine serum and resazurin, were incubated with 5, 10, 20, and 40 μM HBD3. Cytotoxicity was determined by measuring metabolic conversion of resazurin to resorufin. The lethal dose 50 (LD50, mean μM±Std Err) values were determined from the median fluorescent intensities of test concentrations compared to live and killed cell controls. The LD50 value range of HBD3 was 18.2-35.9 μM in serum-free media for DCs, NHEKs, hTERT keratinocytes, and GE keratinocytes, and >40.0 μM in complete media. Thus, HBD3 was cytotoxic at higher concentrations, which must be considered in future studies of HBD3-modulated chemokine and cytokine responses in vitro. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  3. Use of minimally invasive gingival biopsies in the study of inflammatory mediators expression and their correlation with gingival fluid in patients with chronic periodontitis

    Directory of Open Access Journals (Sweden)

    Karina Schittine Bezerra Lomba

    2015-01-01

    Full Text Available Background: There are no studies comparing the gingival crevicular fluid (GCF cytokines expression with its corresponding values from the same tissue′s sites. Such comparison might be of great value since most of the cytokine function is related to cell and/or tissue receptors. Aims: Our aim was to use minimally invasive biopsies to evaluate the expression of interferon-gamma, interleukin 1 (IL-1 β, IL-6, IL-17A, IL-17F, and their correlation with the expression in gingival fluid in patients with chronic periodontitis. Materials and Methods: The collection of gingival fluid comprised 22 samples from 11 patients (mean age 46.73 ± 10.16 standard deviation years with chronic periodontitis. The collection of biopsies comprised 22 samples from the same patients. Gingival fluid and biopsy were taken from the same site in one shallow and one deep site per patient. Gingival fluid samples were collected with periopaper ® and analyzed using Luminex ® . Biopsies were taken with a 2 mm diameter punch and analyzed for the same mediators using immunohistochemistry. Results: The gingival fluid showed higher amounts for IL-1-β in deep sites. Immunohistochemical markers were observed in the analyzed cells groups, both in deep and shallow sites, without significant differences between them. In the comparative analysis between immunohistochemical markers and GCF, IL-1-β showed high concordance in shallow and deep sites. Conclusions: The use of a standardized punch of 2 mm diameter for periodontal tissue biopsies seems to be suitable for immunohistochemistry analysis and showed that the GCF may not express all the markers in the same proportion at the corresponding tissue.

  4. A comparative study on EDTA and coronaliy advanced flap technique in the treatment of human gingival recessions

    Directory of Open Access Journals (Sweden)

    Khoshkhoo Nejad AA

    2003-08-01

    Full Text Available Statement of Problem: Treatment of gingival recession defect and covering denuded root surfaces is one of the goals in periodontal therapy and several surgical techniques have been suggested in this field."nPurpose: The aim of this study was to perform a comparison on coronaliy repositioned flap procedure with and without the use of ethylenediaminoteraacetic acid (EDTA. 24%, pH=7 in the treatment of"nrecession defects."nMaterial and Methods: In this randomized clinical trial study, 16 patients, aged 17-60 years, with a total of 27 miller class 1 isolated buccal gingival recession type defects of at least 2mm depth, and based"non special criteria were investigated. After initial therapy, surgical recession coverage was performed as coronaliy advanced flap technique and EDTA gel conditioning (test or coronaliy advanced flap alone"n(control. Clinical examination including assessments of oral hygiene, recession depth (RD, recession width (RW, width of keratinized tissue (KT, probing depth (PD and probing attachment level (PAL"nwere performed before and 1, 2, 3 months after surgical treatment."nResults: The mean of initial RD, RW, KT, PT and PAL in the test group was 2.73, 3.17, 3.13, 1.1 and 3.83mm respectively and in the control group was 2.56, 3.03, 3.67, 1.25, 3.92mm respectively. The mean of these parameters 3 months after treatment in the test group were changed to 0.46, 1.97, 2.65, 0.67, 1.1 mm, corresponding figures for control teeth were 0.85, 2.98, 2.75, 1, 1.94, respectively. At 3 months after treatment the mean root coverage amounted to 83% (test and 67% (control which was a statistically significant difference (P=0.0067. Although a significant clinical difference was observed regarinding root coverage level, all other clinical variables were not statistically different, with the exception of probing attachment level (P=0.005."nConclusion: It was suggested that EDTA gel (24%, PLT=7 for 3 minutes as root conditioner and the coronaliy

  5. Effects of octenidine on dental plaque and gingivitis in monkeys.

    Science.gov (United States)

    Shern, R J; Little, W A; Kennedy, J B; Mirth, D B

    1987-09-01

    This investigation monitored the effects of daily oral rinses with octenidine on plaque and gingivitis in five monkeys. Formulations containing 0.5% or 1.0% octenidine or the rinse vehicle placebo were provided daily for 2 weeks. Each week the dentition of each monkey was examined, photographed, and sampled for plaque. All responses exhibited a numerical decrease in mean scores following treatments with each concentration of octenidine, whereas the placebo treatment exerted negligible effects. Decreases in plaque mass were observed after 2 weeks of treatment with 1% octenidine (58%) or 0.5% octenidine (55%) compared with the corresponding baseline values. Similar trends were noted in the extent and thickness of supragingival plaque and its ability to decrease the pH of a sucrose solution. Octenidine treatments reduced the proportions of motile forms in samples of subgingival plaque and also restricted its ability to produce H2S. Slight numerical decreases were seen in the Gingival Index and flow rate of the crevicular fluid. These consistent protective trends suggest that octenidine decreases the pathogenic potential of established plaque.

  6. Analysis of proliferative activity in oral gingival epithelium in immunosuppressive medication induced gingival overgrowth

    Directory of Open Access Journals (Sweden)

    Özdemir B Handan

    2006-05-01

    Full Text Available Abstract Background Drug-induced gingival overgrowth is a frequent adverse effect associated principally with administration of the immunosuppressive drug cyclosporin A and also certain antiepileptic and antihypertensive drugs. It is characterized by a marked increase in the thickness of the epithelial layer and accumulation of excessive amounts of connective tissue. The mechanism by which the drugs cause gingival overgrowth is not yet understood. The purpose of this study was to compare proliferative activity of normal human gingiva and in cyclosporine A-induced gingival overgrowth. Methods Gingival samples were collected from 12 generally healthy individuals and 22 Cyclosporin A-medicated renal transplant recipients. Expression of proliferating cell nuclear antigen was evaluated in formalin-fixed, paraffin-embedded gingival samples using an immunoperoxidase technique and a monoclonal antibody for this antigen. Results There were differences between the Cyclosporin A group and control group in regard to proliferating cell nuclear antigen and epithelial thickness. In addition, the degree of stromal inflammation was higher in the Cyclosporin A group when compared with the control group. Conclusion The results suggest that the increased epithelial thickness observed in Cyclosporin A-induced gingival overgrowth is associated with increased proliferative activity in keratinocytes.

  7. Amlodipine induced gingival enlargement

    Directory of Open Access Journals (Sweden)

    Shankar Gittaboyina

    2016-01-01

    Full Text Available Drug-induced gingival overgrowth or enlargement is an abnormal growth of the gingiva due to an adverse drug reaction in patients treated with anticonvulsants, immunosuppressants, and calcium channel blockers (CCBs. CCBs are considered as one of the etiologic factors among patients seeking dental care for drug-induced gingival enlargement or overgrowth. This enlargement can be localized or generalized and can range from mild to extremely severe, affecting patient's appearance, and function. CCBs are one of the most commonly used drugs for the management of cardiovascular disorders and are known for causing gingival over growth. Amlodipine is a new CCB and has been used with increasing frequency in the management of hypertension and angina. Although amlodipine is considered as a safe drug, very rarely it may induce gingival overgrowth. A rare case of amlodipine-induced gingival overgrowth has been reported herein a 45-year-old female patient. The treatment aspect included scaling and root planing, substitution of the drug, the surgical excision, and the maintenance and supportive therapy resulting in an excellent clinical outcome.

  8. 改良人牙龈上皮细胞原代培养方法%An modified culture method of primary human gingival epithelial cells

    Institute of Scientific and Technical Information of China (English)

    余婧婷; 孟焕新; 刘凯宁

    2016-01-01

    Objective:To establish a stable primary culture method of human gingival epithelial cells, with a higher successful rate and shorter culture time.Methods:Nine patients who received “crown-lengthening surgery”with relatively healthy periodontal conditions were selected (n =9).Gingival sam-ples were collected from the 9 donors during gingivectomy.Gingival epithelial cells were isolated and cul-tured by both an advanced enzyme digestion method and a tissue explant method.In the advanced enzyme digestion culture process,2.5 g/L DispaseⅡwas used to separate the epithelial tissue part from the con-nective tissue part,which lasted for one night.Then the epithelial tissues were digested by 0.025% tryp-sin without EDTA for 10 minutes,and centrifuged by keeping the digested epithelial tissues that re-mained.This advanced method not only decreased the concentration and digesting time of the two above-mentioned enzymes,but also simplified the centrifugel process.The tissue explant method was not changed too much compared with the original method.Growing processes of the primary cells cultured by the two methods were observed and recorded respectively,and indirect immunocytochemical staining was used to identify the type of cultured cells.At the same time,successful rates and cell culture time were also compared between the two methods.Results:Human gingival epithelial cells with typical morpholo-gy could be cultured within a shorter period by the advanced enzyme digestion method with a successful rate of 88.9%,and proliferated rapidly as sheets.After 10 -14 d cells could be passaged,gradually turned to be like fibroblasts when passaged to the third generation,and eventually went to apoptosis.The primary culture time was longer by using the tissue explant method,and approximately after 17 -22 d cells could be passaged,although the successful rate was the same as the enzyme digestion method.Cy-tokeratin staining was both positive by indirect immunocytochemical staining of

  9. Gingival Recessions and Biomechanics

    DEFF Research Database (Denmark)

    Laursen, Morten Godtfredsen

    Gingival recessions and biomechanics “Tissue is the issue, but bone sets the tone.“ A tooth outside the cortical plate can result in loss of bone and development of a gingival recession. The presentation aims to show biomechanical considerations in relation to movement of teeth with gingival...... by moving the root back in the alveolus. The tooth movement is accompanied by bone gain and thus increase the success rate for soft tissue augmentation. The choice of biomechanical system influences the treatment outcome. If a standard straight wire appliance is used, a biomechanical dilemma can arise....... The forces applied to bring the tooth back into the alveolar process generate opposite reactive forces, which can direct the adjacent teeth out towards the boundary of the bony envelope. A different force system can be achieved with a segmented appliance: The reaction forces from the root movement...

  10. DESQUAMATIVE GINGIVITIS: A REVIEW

    Directory of Open Access Journals (Sweden)

    Gizem KARAGÖZ

    2016-04-01

    Full Text Available Desquamative gingivitis (DG is characterized by the erythematous gingiva, desquamation and erosion of the gingival epithelium, and blister formation. It is a common clinical manifestation in several diseases. Contact allergic reactions to various oral hygiene products and chemical agents have also been reported to represent as DG. The management of DG has been a major problem, largely because the etiology of the disease has been elusive. In this paper, we aimed to review the current literature on the pathogenesis, diagnosis management and prognosis of DG.

  11. Expression levels of novel cytokine IL-32 in periodontitis and its role in the suppression of IL-8 production by human gingival fibroblasts stimulated with Porphyromonas gingivalis

    Directory of Open Access Journals (Sweden)

    Kazuhisa Ouhara

    2012-03-01

    Full Text Available Background:IL-32 was recently found to be elevated in the tissue of rheumatoid arthritis and inflammatory bowel disease. Periodontitis is a chronic inflammatory disease caused by polymicrobial infections that result in soft tissue destruction and alveolar bone loss. Although IL-32 is also thought to be associated with periodontal disease, its expression and possible role in periodontal tissue remain unclear. Therefore, this study investigated the expression patterns of IL-32 in healthy and periodontally diseased gingival tissue. The expression of IL-32 in cultured human gingival fibroblasts (HGF as well as effects of autocrine IL-32 on IL-8 production from HGF were also examined.Methods:Periodontal tissue was collected from both healthy volunteers and periodontitis patients, and immunofluorescent staining was performed in order to determine the production of IL-32. Using real-time PCR and ELISA, mRNA expression and protein production of IL-32 in HGF, stimulated by Porphyromonas gingivalis (Pg, were also investigated.Results:Contrary to our expectation, the production of IL-32 in the periodontitis patients was significantly lower than in the healthy volunteers. According to immunofluorescent microscopy, positive staining for IL-32 was detected in prickle and basal cell layers in the epithelium as well as fibroblastic cells in connective tissue. Addition of fixed Pg in vitro was found to suppress the otherwise constitutive expression of IL-32 mRNA and protein in HGF. However, recombinant IL-32 in vitro inhibited the expression of IL-8 mRNA by HGF stimulated with Pg. Interestingly, anti-IL-32 neutralizing antibody upregulated the IL-8 mRNA expression in non-stimulated HGF, indicating that constitutive expression of IL-32 in HGF suppressed IL-8 mRNA expression in the absence of bacterial stimulation.Conclusion:These results indicate that IL-32 is constitutively produced by HGF which can be suppressed by Pg and may play a role in the downregulation

  12. Evaluation of Wound Closure Activity of Nigella sativa, Melastoma malabathricum, Pluchea indica, and Piper sarmentosum Extracts on Scratched Monolayer of Human Gingival Fibroblasts

    Directory of Open Access Journals (Sweden)

    Mas Rizal Ab Rahman

    2014-01-01

    Full Text Available Nigella sativa, Melastoma malabathricum, Pluchea indica, and Piper sarmentosum are common Asian traditional medicines to treat minor wounds. This study aimed to investigate the in vitro wound healing properties of aqueous extracts of these plants using human gingival fibroblast (HGF monolayer as study model. DPPH scavenging activity of the extracts was evaluated and effect on HGF proliferation was determined. Their effect on HGF’s function to synthesize collagen was indicated by the level of hydroxyproline produced and effect on wound healing activity was assessed using an in vitro scratch assay. The influence of the extracts on expression of bFGF and TGF-β was also determined. Results revealed all four extracts to exhibit low free radical scavenging activity. The extract from N. sativa (NSSE compared to the others showed favourable enhancement of HGF proliferation with EC50 of 22.67±3.06 µg/mL (P<0.05 with accelerated wound closure activity despite its nonsignificant effect on collagen synthesis. In addition to the elevated level of bFGF by up to 15% at 100 µg/mL of NSSE, a slightly better effect was observed on the expression of TGF-β. NSSE thus showed that promising wound healing properties and data obtained may contribute towards validation of its traditional use for the healing of oral wounds.

  13. Modulation of human gingival fibroblast adhesion, morphology, tyrosine phosphorylation, and ERK 1/2 localization on polished, grooved and SLA substratum topographies.

    Science.gov (United States)

    Kokubu, Eitoyo; Hamilton, Douglas W; Inoue, Takashi; Brunette, Donald M

    2009-12-01

    Attachment of connective tissue to dental implants, which is influenced by surface topography, is an important determinant of implant success. Approaches employed to alter topography include acid etching or blasting to produce roughened surfaces, and production of precisely defined topographies using microfabrication techniques. The aim of this study was to assess the influence of polished, microgrooved, and sand-blasted, large grit, acid-etched (SLA) topographies on fibroblast adhesion, morphology, activation, and ERK 1/2 phosphorylation and localization. Human gingival fibroblasts (HGFs) spread on all tested surfaces within 2 h, and topography influenced the pattern of phosphotyrosine localization. Fibrillar adhesion formation was prominent in HGFs cultured on microgrooves and SLA at 24 h compared with smooth. No significant difference in ERK 1/2 phosphorylation was observed at 2 or 24 h, but nuclear localization depended on culture time and substratum topography. Nuclear localization of ERK 1/2 occurred at 2 h on polished surfaces, but was not evident at 1 week. In contrast, cells on SLA and grooved surfaces did not exhibit nuclear localization of ERK 1/2 at early times, but did at 1 week. The results of this study suggest that rough and microfabricated topographies influence fibroblast adhesion and intracellular signaling through focal adhesion/integrin-dependent mechanisms in a time-dependent manner. Copyright 2008 Wiley Periodicals, Inc.

  14. In vitro systems toxicology approach to investigate the effects of repeated cigarette smoke exposure on human buccal and gingival organotypic epithelial tissue cultures

    Science.gov (United States)

    Schlage, Walter K.; Kostadinova, Radina; Xiang, Yang; Sewer, Alain; Majeed, Shoaib; Kuehn, Diana; Frentzel, Stefan; Talikka, Marja; Geertz, Marcel; Mathis, Carole; Ivanov, Nikolai; Hoeng, Julia; Peitsch, Manuel C.

    2014-01-01

    Smoking has been associated with diseases of the lung, pulmonary airways and oral cavity. Cytologic, genomic and transcriptomic changes in oral mucosa correlate with oral pre-neoplasia, cancer and inflammation (e.g. periodontitis). Alteration of smoking-related gene expression changes in oral epithelial cells is similar to that in bronchial and nasal epithelial cells. Using a systems toxicology approach, we have previously assessed the impact of cigarette smoke (CS) seen as perturbations of biological processes in human nasal and bronchial organotypic epithelial culture models. Here, we report our further assessment using in vitro human oral organotypic epithelium models. We exposed the buccal and gingival organotypic epithelial tissue cultures to CS at the air–liquid interface. CS exposure was associated with increased secretion of inflammatory mediators, induction of cytochrome P450s activity and overall weak toxicity in both tissues. Using microarray technology, gene-set analysis and a novel computational modeling approach leveraging causal biological network models, we identified CS impact on xenobiotic metabolism-related pathways accompanied by a more subtle alteration in inflammatory processes. Gene-set analysis further indicated that the CS-induced pathways in the in vitro buccal tissue models resembled those in the in vivo buccal biopsies of smokers from a published dataset. These findings support the translatability of systems responses from in vitro to in vivo and demonstrate the applicability of oral organotypical tissue models for an impact assessment of CS on various tissues exposed during smoking, as well as for impact assessment of reduced-risk products. PMID:25046638

  15. Increased cell proliferation and differential protein expression induced by low-level Er:YAG laser irradiation in human gingival fibroblasts: proteomic analysis.

    Science.gov (United States)

    Ogita, Mayumi; Tsuchida, Sachio; Aoki, Akira; Satoh, Mamoru; Kado, Sayaka; Sawabe, Masanori; Nanbara, Hiromi; Kobayashi, Hiroaki; Takeuchi, Yasuo; Mizutani, Koji; Sasaki, Yoshiyuki; Nomura, Fumio; Izumi, Yuichi

    2015-09-01

    Erbium-doped yttrium aluminum garnet (Er:YAG) laser treatment has demonstrated favorable wound healing effect after periodontal therapy. One of the reasons may be the positive biological effect of the low-level laser on the irradiated tissues, although the mechanism remains unclear. The aim of this study was to investigate the effect of low-level Er:YAG laser irradiation on cell proliferation and laser-induced differential expression of proteins in human gingival fibroblasts (HGFs) by proteomic analysis. In the first experiment, HGFs were exposed to low-level Er:YAG laser irradiation and the laser-induced cell proliferation and damage were evaluated on day 3. In the second experiment, proteomic analysis was performed on day 1 after irradiation. The peptides prepared from HGFs were analyzed by a hybrid ion trap-Fourier transform mass spectrometer, Mascot search engine, and UniProtKB database. A significant increase in cell proliferation without cell damage after irradiation was observed. Among the total identified 377 proteins, 59 proteins, including galectin-7, which was associated with the process of wound healing, were upregulated and 15 proteins were downregulated in laser-treated HGFs. In the third experiment, the increase in messenger RNA (mRNA) and protein expression of galectin-7 in the irradiated HGFs was validated by various analytical techniques. In addition, the effect of recombinant human galectin-7 on the modulation of HGFs proliferation was confirmed. The results indicate that low-level Er:YAG laser irradiation can promote HGF proliferation and induce a significant change in protein expression and the upregulation of galectin-7 expression may partly contribute to the increase in cell proliferation.

  16. In vitro systems toxicology approach to investigate the effects of repeated cigarette smoke exposure on human buccal and gingival organotypic epithelial tissue cultures.

    Science.gov (United States)

    Schlage, Walter K; Iskandar, Anita R; Kostadinova, Radina; Xiang, Yang; Sewer, Alain; Majeed, Shoaib; Kuehn, Diana; Frentzel, Stefan; Talikka, Marja; Geertz, Marcel; Mathis, Carole; Ivanov, Nikolai; Hoeng, Julia; Peitsch, Manuel C

    2014-10-01

    Smoking has been associated with diseases of the lung, pulmonary airways and oral cavity. Cytologic, genomic and transcriptomic changes in oral mucosa correlate with oral pre-neoplasia, cancer and inflammation (e.g. periodontitis). Alteration of smoking-related gene expression changes in oral epithelial cells is similar to that in bronchial and nasal epithelial cells. Using a systems toxicology approach, we have previously assessed the impact of cigarette smoke (CS) seen as perturbations of biological processes in human nasal and bronchial organotypic epithelial culture models. Here, we report our further assessment using in vitro human oral organotypic epithelium models. We exposed the buccal and gingival organotypic epithelial tissue cultures to CS at the air-liquid interface. CS exposure was associated with increased secretion of inflammatory mediators, induction of cytochrome P450s activity and overall weak toxicity in both tissues. Using microarray technology, gene-set analysis and a novel computational modeling approach leveraging causal biological network models, we identified CS impact on xenobiotic metabolism-related pathways accompanied by a more subtle alteration in inflammatory processes. Gene-set analysis further indicated that the CS-induced pathways in the in vitro buccal tissue models resembled those in the in vivo buccal biopsies of smokers from a published dataset. These findings support the translatability of systems responses from in vitro to in vivo and demonstrate the applicability of oral organotypical tissue models for an impact assessment of CS on various tissues exposed during smoking, as well as for impact assessment of reduced-risk products.

  17. Treatment of gingival recession using free gingival graft with fibrin fibronectin sealing system: A novel approach.

    Science.gov (United States)

    Srinivas, B V V; Rupa, N; Halini Kumari, K V; Rajender, A; Reddy, M Narendra

    2015-08-01

    Periodontal plastic surgery is the branch of periodontology that is focused mainly on the correction or elimination of mucogingival problems associated with lack of attached gingiva, a shallow vestibule and aberrant frenum. Various mucogingival surgical procedures are used to halt the progression of the gingival recession and to correct poor esthetic appearance. Free gingival autograft is one of the most common techniques used for a gingival recession in areas of inadequate attached gingiva in the mandibular anterior region. Fibrin sealants are human plasma derivatives that mimic the final stages of blood coagulation, forming a fibrin clot. Fibrin Sealants enhances the overall outcome of surgical intervention because of their hemostatic, adhesive, and healing properties. These properties of fibrin sealants may reduce operating time, prevent complications, and enhance the overall outcome of many surgical interventions. Hence, this case report aims to investigate the clinical effectiveness of free gingival graft along with the commercially available fibrin-fibronectin sealing system (Tissucol(®)) in the treatment of Miller's class II gingival recession.

  18. Synergistic anti-inflammatory activity of the antimicrobial peptides human beta-defensin-3 (hBD-3 and cathelicidin (LL-37 in a three-dimensional co-culture model of gingival epithelial cells and fibroblasts.

    Directory of Open Access Journals (Sweden)

    Telma Blanca Lombardo Bedran

    Full Text Available Given the spread of antibiotic resistance in bacterial pathogens, antimicrobial peptides that can also modulate the immune response may be a novel approach for effectively controlling periodontal infections. In the present study, we used a three-dimensional (3D co-culture model of gingival epithelial cells and fibroblasts stimulated with Aggregatibacter actinomycetemcomitans lipopolysaccharide (LPS to investigate the anti-inflammatory properties of human beta-defensin-3 (hBD-3 and cathelicidin (LL-37 and to determine whether these antimicrobial peptides can act in synergy. The 3D co-culture model composed of gingival fibroblasts embedded in a collagen matrix overlaid with gingival epithelial cells had a synergistic effect with respect to the secretion of IL-6 and IL-8 in response to LPS stimulation compared to fibroblasts and epithelial cells alone. The 3D co-culture model was stimulated with non-cytotoxic concentrations of hBD-3 (10 and 20 µM and LL-37 (0.1 and 0.2 µM individually and in combination in the presence of A. actinomycetemcomitans LPS. A multiplex ELISA assay was used to quantify the secretion of 41 different cytokines. hBD-3 and LL-37 acted in synergy to reduce the secretion of GRO-alpha, G-CSF, IP-10, IL-6, and MCP-1, but only had an additive effect on reducing the secretion of IL-8 in response to A. actinomycetemcomitans LPS stimulation. The present study showed that hBD-3 acted in synergy with LL-37 to reduce the secretion of cytokines by an LPS-stimulated 3D model of gingival mucosa. This combination of antimicrobial peptides thus shows promising potential as an adjunctive therapy for treating inflammatory periodontitis.

  19. Gingival plasma cell granuloma

    Directory of Open Access Journals (Sweden)

    Amitkumar B Pandav

    2012-01-01

    Full Text Available Plasma cell granuloma, also known as inflammatory pseudotumor is a tumor-like lesion that manifests primarily in the lungs. But it may occur in various other anatomic locations like orbit, head and neck, liver and rarely in the oral cavity. We here report an exceedingly rare case of gingival plasma cell granuloma in a 58 year old woman who presented with upper gingival polypoidal growth. The histopathological examination revealed a mass composed of proliferation of benign spindle mesenchymal cells in a loose myxoid and fibrocollagenous stroma along with dense infiltrate of chronic inflammatory cells predominantly containing plasma cells. Immunohistochemistry for kappa and lambda light chains showed a polyclonal staining pattern confirming a diagnosis of plasma cell granuloma.

  20. Idiopathic gingival fibromatosis

    Directory of Open Access Journals (Sweden)

    Sujata Rath

    2011-01-01

    This article presents a case report of a 14-year-old female patient with idiopathic gingival fibromatosis in the maxillary region with radiographic feature of congenitally missing maxillary permanent left lateral incisor, maxillary left and right permanent canine, mandibular right second premolar, all third molars along with overretained primary maxillary left lateral incisor and primary mandibular second molar. The treatment rendered in this patient comprised of surgical excision of the hyperplasia under general anesthesia.

  1. Association of serum and crevicular visfatin levels in periodontal health and disease with type 2 diabetes mellitus.

    Science.gov (United States)

    Pradeep, A R; Raghavendra, N M; Sharma, Anuj; Patel, Swati Pradeep; Raju, Arjun; Kathariya, Rahul; Rao, Nishanth S; Naik, Savitha B

    2012-05-01

    Levels of visfatin in serum and gingival crevicular fluid (GCF) were explored in patients with periodontal health, periodontal disease with and without type 2 diabetes mellitus (t2 DM) and were found to be elevated with periodontal disease, and were correlated with periodontal clinical parameters. DM and chronic periodontitis (CP) are associated with each other. Adipokines, specifically visfatin, are secreted from adipocytes and are thought to cause insulin resistance. The purpose of this study is to determine the presence of visfatin in serum and GCF in t2 DM among individuals with CP and to find an association, if any. Thirty individuals (15 males and 15 females) were selected based on their clinical parameters into three groups: group 1 (10 healthy), group 2 (10 well-controlled t2 DM among individuals with CP), and group 3 (10 individuals with CP and without diabetes). Serum and GCF samples were collected to estimate the levels of visfatin using enzyme linked immunosorbent assay. The mean visfatin concentration increased in both serum and GCF in individuals with t2 DM with CP. Also, it was observed that visfatin in both serum and GCF correlated positively with all the periodontal parameters. All the samples in each group tested positive for visfatin assay. Serum and GCF visfatin concentration in both t2 DM with CP and individuals with CP and without diabetes correlated positively with all the clinical parameters. Additional large-scale longitudinal studies should be performed to confirm positive correlations.

  2. 4-Hydroxy-2-nonenal, an oxidative stress marker in crevicular fluid and serum in type 2 diabetes with chronic periodontitis

    Directory of Open Access Journals (Sweden)

    A R Pradeep

    2013-01-01

    Full Text Available Objective: Chronic periodontitis (CP is a common, chronic inflammatory disease initiated by bacteria, which has an increased prevalence and severity in patients with type 2 diabetes mellitus (t2 DM. A variety of reactive oxygen species are able to cause direct damage to proteins, deoxyribonucleic acid, carbohydrates and lipids. Lipid peroxidation is always combined with the formation of reactive aldehydes like 4-Hydroxy-2-nonenal (HNE. The purpose of this study was to determine the presence of the HNE-His adducts levels in serum and gingival crevicular fluid (GCF in t2 DM among CP subjects and to find an association, if any. Materials and Methods: A total of 40 subjects (20 males and 20 females were selected based on their clinical parameters into three groups: Group 1 (10 healthy, Group 2 (15 subjects, CP without t2 DM, Group 3 (15 subjects, CP with t2 DM. Serum and GCF samples were collected to estimate the levels of the HNE-His adducts by the enzyme linked immunosorbent assay. Results: The mean HNE-His adducts concentration both in serum and GCF was highest for Group 3 followed by Group 2 and least in Group 1. Conclusions: All samples in each group tested positive for HNE-His adducts assay. Serum and GCF HNE-His adducts concentration both in t2 DM with CP and non-diabetic CP subjects were higher than the healthy controls. Further large scale longitudinal studies should be carried out to confirm positive correlations.

  3. In vitro effects of Choukroun's PRF (platelet-rich fibrin) on human gingival fibroblasts, dermal prekeratinocytes, preadipocytes, and maxillofacial osteoblasts in primary cultures.

    Science.gov (United States)

    Dohan Ehrenfest, David M; Diss, Antoine; Odin, Guillaume; Doglioli, Pierre; Hippolyte, Marie-Pascale; Charrier, Jean-Baptiste

    2009-09-01

    The objective of this study was to analyze the effects of Choukroun's PRF (platelet-rich fibrin), a leucocyte and platelet concentrate clinically usable as fibrin membrane or clot, on human primary cultures of gingival fibroblasts, dermal prekeratinocytes, preadipocytes, and maxillofacial osteoblasts. For the proliferation study, these cells were cultivated with or without a PRF membrane originating from the same donor as for the cells. For osteoblasts and fibroblasts, dose-dependent effect was assessed (using 2 membranes). Cell counts and cytotoxicity tests were performed at 3, 7, 14, and 21 days, and even 28 days for osteoblasts. More osteoblast cultures were prepared in differentiation conditions according to 3 modalities (without PRF, with PRF, with PRF the first day and differentiation medium applied only after the first week of culture). Osteoblast differentiation was analyzed using Von Kossa staining and alkaline phosphatase, DNA and total cell proteins dosage. PRF induced a significant and continuous stimulation of proliferation in all cell types. It was dose dependent during all the experiment with osteoblasts, but only on day 14 with fibroblasts. Moreover, PRF induced a strong differentiation in the osteoblasts, whatever the culture conditions. The analysis of osteoblast cultures in differentiation conditions with PRF, using light and scanning electron microscopy, revealed a starting mineralization process in the PRF membrane itself after 14 days. Moreover, PRF leucocytes seemed to proliferate and interact with osteoblasts. Cultures with PRF are always cocultures with leucocytes. These "chaperone leucocytes" could be the source of differential geographic regulation within the culture and explain the double contradictory effect proliferation/differentiation observed on osteoblasts.

  4. Biological responses of human gingival fibroblasts (HGFs in an innovative co-culture model with Streptococcus mitis to thermosets coated with a silver polysaccharide antimicrobial system.

    Directory of Open Access Journals (Sweden)

    Silvia Sancilio

    Full Text Available This study sought to evaluate the in vitro biological response of human gingival fibroblasts (HGFs co-coltured with Streptococcus mitis to bisphenol A glycidylmethacrylate/triethylene glycol dimethacrylate (BisGMA/TEGDMA thermosets coated with Chitlac-nAg, a nanocomposite system with antimicrobial properties. To avoid bacterial adhesion to dental devices and to reduce cytotoxicity against eukaryotic cells, we coated BisGMA/TEGDMA methacrylic thermosets with a new material, Chitlac-nAg, formed by stabilizing silver nanoparticles, which have well-known antimicrobial properties, with a polyelectrolyte solution containing Chitlac. Cytotoxicity, cell morphology, cell migration and inflammatory interleukine-6 (IL-6 and prostaglandin E2 (PGE2 secretion were evaluated. Our results showed that the cytotoxicity exerted on HGFs by our nanocomposite material was absent in our co-culture model, where fibroblasts are able to adhere and migrate. After 24 h thermosets coated with Chitlac as well as those coated with Chitlac-nAg exerted a minimal cytotoxic effect on HGFs, while after 48 h LDH release rises up 20%. Moreover the presence of S. mitis reduced this release in a greater amount with Chitlac-nAg coated thermosets. The secretion of IL-6 was significant in both Chitlac and Chitlac-nAg coated thermosets, but PGE2 production was minimal, suggesting that the IL-6 production was not related to an inflammatory response. Co-culture and the addiction of saliva did not influence IL-6 and PGE2 secretion. Data obtained in the present work suggest that Chitlac n-Ag coated thermosets could significantly improve the success rates of restorative dentistry, since they limit bacterial adhesion and are not toxic to HGFs.

  5. Cytotoxic Evaluation of Elastomeric Dental Impression Materials on a Permanent Mouse Cell Line and on a Primary Human Gingival Fibroblast Culture

    Directory of Open Access Journals (Sweden)

    Roberta Tiozzo

    2009-08-01

    Full Text Available The need for clinically relevant in vitro tests of dental materials is widely recognized. Nearly all dental impression materials are introduced into the mouth just after mixing and allowed to set in contact with the oral tissues. Under these conditions, the materials may be toxic to cells or may sensitize the tissues. The aim of the present study is to evaluate the potential cytotoxicity of new preparations of elastomeric dental impression materials: A four vinylpolysiloxanes: Elite H-D Putty and Elite H-D Light Body (Zhermack, Badia Polesine, Rovigo, Italy; Express Putty and Express Light Body (3M ESPE AG Seefeld, Germany and B two polyethers: Impregum Penta and Permadyne Penta L (3M ESPE AG Seefeld, Germany. The cytotoxicity of these impression materials were examined using two different cell lines: Balb/c 3T3 (permanent cell line and human gingival fibroblasts (primary cell line and their effects were studied by indirect and direct tests. The direct tests are performed by placing one sample of the impression materials in the centre of the Petri dishes at the time of the seeding of cells. The cell growth was evaluated at the 12th and 24th hours by cell number. The indirect tests were performed by incubating a square of 1 cm diameter impression material in 5 mL of medium at 37 °C for 24 hours (“eluates”. Subconfluent cultures are incubated with “eluates” for 24 hours. The MTT-formazan production is the method used for measuring the cell viability. The results indicate that: a polyether materials are cytotoxic under both experimental conditions; b among vinylpolysiloxanes, only Express Light Body (3M ESPE AG Seefeld, Germany induces clear inhibition of cellular viability of Balb/c 3T3 evaluated by direct and indirect tests and c the primary cell line is less sensitive to the toxic effect than the permanent cell line.

  6. Engineered collagen hydrogels for the sustained release of biomolecules and imaging agents: promoting the growth of human gingival cells.

    Science.gov (United States)

    Choi, Jonghoon; Park, Hoyoung; Kim, Taeho; Jeong, Yoon; Oh, Myoung Hwan; Hyeon, Taeghwan; Gilad, Assaf A; Lee, Kwan Hyi

    2014-01-01

    We present here the in vitro release profiles of either fluorescently labeled biomolecules or computed tomography contrast nanoagents from engineered collagen hydrogels under physiological conditions. The collagen constructs were designed as potential biocompatible inserts into wounded human gingiva. The collagen hydrogels were fabricated under a variety of conditions in order to optimize the release profile of biomolecules and nanoparticles for the desired duration and amount. The collagen constructs containing biomolecules/nanoconstructs were incubated under physiological conditions (ie, 37°C and 5% CO2) for 24 hours, and the release profile was tuned from 20% to 70% of initially loaded materials by varying the gelation conditions of the collagen constructs. The amounts of released biomolecules and nanoparticles were quantified respectively by measuring the intensity of fluorescence and X-ray scattering. The collagen hydrogel we fabricated may serve as an efficient platform for the controlled release of biomolecules and imaging agents in human gingiva to facilitate the regeneration of oral tissues.

  7. Salivary agglutinin is the major component in human saliva that modulates the lectin pathway of the complement system

    NARCIS (Netherlands)

    Gunput, S.T.G.; Wouters, D.; Nazmi, K.; Cukkemane, N.; Brouwer, M.; Veerman, E.C.I.; Ligtenberg, A.J.M.

    2016-01-01

    Saliva interacts with blood after mucosal damage or leakage of gingival crevicular fluid. Surface-adsorbed salivary agglutinin (SAG) activates the lectin pathway (LP) of the complement system via mannose-binding lectin, while SAG in solution inhibits complement activation. In the present study we

  8. Comparative analysis of gingival phenotype in animal and human experimental models using optical coherence tomography in a non-invasive approach

    Science.gov (United States)

    Mota, Cláudia C. B. O.; Fernandes, Luana O.; Melo, Luciana S. A.; Feitosa, Daniela S.; Cimões, Renata; Gomes, Anderson S. L.

    2015-06-01

    Imaging methods are widely used in diagnostic and among the diversity of modalities, optical coherence tomography (OCT) is nowadays commercially available and considered the most innovative technique used for imaging applications, in both medical and non-medical applications. In this study, we exploit the OCT technique in the oral cavity for identification and differentiation between free and attached gingiva, as well as determining the gingival phenotype, an important factor to determination of periodontal prognosis in patients. For the animal studies, five porcine jaws were analyzed using a Swept Source SS-OCT system operating at 1325nm and stereomicroscope, as gold pattern. The SSOCT at 1325nm was chosen due to the longer central wavelength, that allows to deeper penetration imaging, and the faster image acquisition, an essential factor for clinical setting. For the patient studies, a total of 30 males and female were examined using the SS-OCT at 1325nm and computer controlled periodontal probing. 2D and 3D images of tooth/gingiva interface were performed, and quantitative measurements of the gingival sulcus could be noninvasively obtained. Through the image analysis of the animals jaws, it was possible to quantify the free gingiva and the attached gingiva, the calculus deposition over teeth surface and also the subgingival calculus. For the patient's studies, we demonstrated that the gingival phenotype could be measured without the periodontal probe introduction at the gingival sulcus, confirming that OCT can be potentially useful in clinic for direct observation and quantification of gingival phenotype in a non-invasive approach.

  9. LACK OF ASSOCIATION BETWEEN HERPESVIRUS DETECTION IN SALIVA AND GINGIVITIS IN HIV‑INFECTED CHILDREN

    OpenAIRE

    OTERO, Renata A.; Flávia N.N. NASCIMENTO; SOUZA,Ivete P.R.; SILVA,Raquel C.; LIMA,Rodrigo S.; ROBAINA,Tatiana F.; CÂMARA, Fernando P.; Santos, Norma; CASTRO,Gloria F.

    2015-01-01

    The aims of this study were to compare the detection of human herpesviruses (HHVs) in the saliva of HIV-infected and healthy control children, and to evaluate associations between viral infection and gingivitis and immunodeficiency. Saliva samples were collected from 48 HIV-infected and 48 healthy control children. Clinical and laboratory data were collected during dental visits and from medical records. A trained dentist determined gingival indices and extension of gingivitis. Saliva samples...

  10. Sex Hormones Enhance Gingival Inflammation without Affecting IL-1β and TNF-α in Periodontally Healthy Women during Pregnancy

    Directory of Open Access Journals (Sweden)

    Min Wu

    2016-01-01

    Full Text Available Hormones (progesterone and estradiol change greatly during pregnancy; however, the mechanism of hormonal changes on gingival inflammation is still unclear. This study is to evaluate the effects of hormonal changes during pregnancy on gingival inflammation and interleukin-1β (IL-1β and tumor necrosis factor-α (TNF-α in gingival crevicular fluid (GCF. 30 periodontally healthy pregnant women were evaluated in the first, second, and third trimesters. 20 periodontally healthy nonpregnant women were evaluated twice (once per subsequent month. Clinical parameters including probing pocket depth (PPD, bleeding index (BI, gingival index (GI, clinical attachment level (CAL, and plaque index (PLI were recorded. GCF levels of IL-1β and TNF-α and serum levels of progesterone and estradiol were measured. From the data, despite low PLI, BI and GI increased significantly during pregnancy; however, no significant changes in PLI, CAL, IL-1β, or TNF-α GCF levels were observed. Although IL-1β, not TNF-α, was higher in pregnant group than in nonpregnant group, they showed no correlation with serum hormone levels during pregnancy. GI and BI showed significant positive correlation with serum hormone levels during pregnancy. This study suggests that sex hormone increase during pregnancy might have an effect on inflammatory status of gingiva, independent of IL-1β and TNF-α in GCF.

  11. Sex Hormones Enhance Gingival Inflammation without Affecting IL-1β and TNF-α in Periodontally Healthy Women during Pregnancy.

    Science.gov (United States)

    Wu, Min; Chen, Shao-Wu; Su, Wei-Lan; Zhu, Hong-Ying; Ouyang, Shu-Yuan; Cao, Ya-Ting; Jiang, Shao-Yun

    2016-01-01

    Hormones (progesterone and estradiol) change greatly during pregnancy; however, the mechanism of hormonal changes on gingival inflammation is still unclear. This study is to evaluate the effects of hormonal changes during pregnancy on gingival inflammation and interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in gingival crevicular fluid (GCF). 30 periodontally healthy pregnant women were evaluated in the first, second, and third trimesters. 20 periodontally healthy nonpregnant women were evaluated twice (once per subsequent month). Clinical parameters including probing pocket depth (PPD), bleeding index (BI), gingival index (GI), clinical attachment level (CAL), and plaque index (PLI) were recorded. GCF levels of IL-1β and TNF-α and serum levels of progesterone and estradiol were measured. From the data, despite low PLI, BI and GI increased significantly during pregnancy; however, no significant changes in PLI, CAL, IL-1β, or TNF-α GCF levels were observed. Although IL-1β, not TNF-α, was higher in pregnant group than in nonpregnant group, they showed no correlation with serum hormone levels during pregnancy. GI and BI showed significant positive correlation with serum hormone levels during pregnancy. This study suggests that sex hormone increase during pregnancy might have an effect on inflammatory status of gingiva, independent of IL-1β and TNF-α in GCF.

  12. Gingivitis ulceronecrosante aguda

    Directory of Open Access Journals (Sweden)

    Eduardo de la Teja-Ángeles

    2015-11-01

    Full Text Available La gingivitis ulcerativa necrosante, conocida por sus siglas en inglés como GUN (anteriormente se le conocía como enfermedad de Vincent o “boca de trinchera” por afectar a soldados en guerra, es una enfermedad poco frecuente.1-6 Se caracteriza por ser una infección aguda y dolorosa en la que las encías sangran, hay necrosis de las papilas interdentales y ataque al estado general.

  13. Alteration in the phagocyte activity of crevicular leucocytes regarding diabetic patients with periodontal disease

    OpenAIRE

    Colchado Carhuavilca, Jorge R.; Departamento Académico Medico quirúrgico. Facultad Odontología. UNMSM. Lima Perú.

    2014-01-01

    The goal of this investigation was to find out the alterations in the phagocyte activity of crevicular leucocytes regarding to diabetic patients with periodontal disease. For that reason, 56 patients with periodontal disease were selected: 42 of them with a diagnosis of Diabetes Mellitus - Type 2, and 14 patients without it (control group), from 35 to 74 years old. To performed the study, crevicular fluid of the mentioned patients was analyzed. By means of sequential washings the samples were...

  14. Interleukin-4 inhibition of interleukin-1-induced expression of matrix metalloproteinase-3 (MMP-3 is independent of lipoxygenase and PPARγ activation in human gingival fibroblasts

    Directory of Open Access Journals (Sweden)

    Gorski Grzegorz

    2007-02-01

    Full Text Available Abstract Background Interleukin 4 (IL-4 has been shown to suppress interleukin-1 (IL-1 induced expression of matrix metalloproteinase-3 (MMP-3 in human synovial and gingival fibroblasts, but the mechanism of suppression has not been determined. Activators of peroxisome proliferator-activated receptor-γ (PPARγ have been shown to inhibit cytokine induced expression of MMPs in other cell types, and IL-4 has been shown to activate PPARγ by stimulating production of ligands through the lipoxygenase pathway. It has been suggested that PPARγ may inhibit expression of MMPs by competing with transcription factor AP-1 for binding to a putative composite binding element in the promoters. The objective of this study was to determine whether the suppressive effects of IL-4 on the IL-1 induced expression of MMP-3 involve activation of lipoxygenase and/or PPARγ. Results Western blotting revealed the presence of PPARγ in nuclear extract of HGF. IL-1 induced binding of nuclear extract to the putative composite PPRE/AP-1 site was diminished in the presence of pioglitazone, but there was no evidence of any change in the composition of the retarded complexes, and no evidence of PPARγ binding to this site. Nordihydroguaiaretic acid (NDGA, a non-selective lipoxygenase inhibitor, and MK886, a specific inhibitor of 5-lipoxygenase, induced MMP-3 expression synergistically with IL-1. However IL-4 was still able to inhibit MMP-3 expression in the presence of NDGA or MK886 and IL-1. Activation of PPARγ with pioglitazone not only failed to inhibit IL-1 induced expression of MMP-3 mRNA, but rather super-induced MMP-3 in the presence of IL-1. PPARγ antagonist GW9662 failed to abolish the suppressive effects of IL-4. Another PPARγ activator, 15-deoxy-Delta12,14prostaglandin J2 (15dPGJ2, also super-induced MMP-3 mRNA, and this was due at least in part to increased transcription. Conclusion IL-4 suppression of IL-1-induced MMP-3 expression in HGF is independent of

  15. Predictive modeling of gingivitis severity and susceptibility via oral microbiota.

    Science.gov (United States)

    Huang, Shi; Li, Rui; Zeng, Xiaowei; He, Tao; Zhao, Helen; Chang, Alice; Bo, Cunpei; Chen, Jie; Yang, Fang; Knight, Rob; Liu, Jiquan; Davis, Catherine; Xu, Jian

    2014-09-01

    Predictive modeling of human disease based on the microbiota holds great potential yet remains challenging. Here, 50 adults underwent controlled transitions from naturally occurring gingivitis, to healthy gingivae (baseline), and to experimental gingivitis (EG). In diseased plaque microbiota, 27 bacterial genera changed in relative abundance and functional genes including 33 flagellar biosynthesis-related groups were enriched. Plaque microbiota structure exhibited a continuous gradient along the first principal component, reflecting transition from healthy to diseased states, which correlated with Mazza Gingival Index. We identified two host types with distinct gingivitis sensitivity. Our proposed microbial indices of gingivitis classified host types with 74% reliability, and, when tested on another 41-member cohort, distinguished healthy from diseased individuals with 95% accuracy. Furthermore, the state of the microbiota in naturally occurring gingivitis predicted the microbiota state and severity of subsequent EG (but not the state of the microbiota during the healthy baseline period). Because the effect of disease is greater than interpersonal variation in plaque, in contrast to the gut, plaque microbiota may provide advantages in predictive modeling of oral diseases.

  16. ASSOCIATION OF EPSTEIN-BARR VIRUS (EBV) BUT NOT HUMAN PAPILLOMAVIRUS (HPV) WITH GINGIVITIS AND/OR PERIODONTITIS IN TRANSPLANTED INDIVIDUALS.

    Science.gov (United States)

    Baez, Camila Freze; Savassi-Ribas, Flavia; Rocha, Wilker Menezes da; Almeida, Stéphanie G S; Gonçalves, Marianna T V; Guimarães, Maria Angelica A M; Cavalcanti, Silvia Maria B; Varella, Rafael B

    2016-07-11

    The aim of this study was to investigate the association of EBV and HPV with gingivitis and/or periodontitis according to the immunologic status. To this end, 74 oral biopsies from transplanted and non-transplanted individuals with the abovementioned oral manifestations were submitted to a screening by PCR for both viruses. According to the results, EBV was strongly associated with gingivitis and/or periodontitis in transplanted individuals (p = 0.011) but not HPV (p = 0.766). EBV-HPV co-detections did not enhance the presence of tissue injury as well. Although a causal relationship was not investigated in this study, the higher frequency of these two oncoviruses in lesion tissues must be investigated in follow-up studies, especially among immunocompromised individuals.

  17. ASSOCIATION OF EPSTEIN-BARR VIRUS (EBV) BUT NOT HUMAN PAPILLOMAVIRUS (HPV) WITH GINGIVITIS AND/OR PERIODONTITIS IN TRANSPLANTED INDIVIDUALS

    Science.gov (United States)

    BAEZ, Camila Freze; SAVASSI-RIBAS, Flavia; da ROCHA, Wilker Menezes; ALMEIDA, Stéphanie G. S.; GONÇALVES, Marianna T. V.; GUIMARÃES, Maria Angelica A. M.; CAVALCANTI, Silvia Maria B.; VARELLA, Rafael B.

    2016-01-01

    SUMMARY The aim of this study was to investigate the association of EBV and HPV with gingivitis and/or periodontitis according to the immunologic status. To this end, 74 oral biopsies from transplanted and non-transplanted individuals with the abovementioned oral manifestations were submitted to a screening by PCR for both viruses. According to the results, EBV was strongly associated with gingivitis and/or periodontitis in transplanted individuals (p = 0.011) but not HPV (p = 0.766). EBV-HPV co-detections did not enhance the presence of tissue injury as well. Although a causal relationship was not investigated in this study, the higher frequency of these two oncoviruses in lesion tissues must be investigated in follow-up studies, especially among immunocompromised individuals. PMID:27410918

  18. Assesment of gingival microcirculation in anterior teeth using laser Doppler flowmetry

    Science.gov (United States)

    Canjau, Silvana; Miron, Mariana I.; Todea, Carmen D.

    2016-03-01

    Introduction: Evaluating the health status of the gingival tissue represents an important objective in the daily practice. Inflammation changes the microcirculatory and micromorphological dynamics of human gingiva. Aim: The purpose of this study was to evaluate the microcirculation in subjects with moderate gingivitis and healthy gingiva by using laser Doppler flowmetry (LDF). Material and Methods: Recordings of the gingival microcirculation (GM) were taken from 20 healthy gingival sites and from 20 sites with moderate gingivitis. The gingival blood flows in the gingivitis group before treatment was significantly different from those in the healthy gingiva group. Signals were recorded with the aid of a laser Doppler MoorLab instrument VMS-LDF2 probe VP3 10 mm S/N 2482. Three consecutive determinations of the GM were registered for each site, as follows: before the initial therapy, at 24 hours after the initial therapy and then, 7 days after the initial therapy. The data were processed using the statistical analysis software SPSS v16.0.1. Results: The results of this preliminary study showed statistically significant differences among the GM values recorded before and after the initial therapy. Conclusions: LDF could be a useful, noninvasive, sensitive, reproducible, and harmless method for measuring gingival blood flow (gingival microcirculation) in humans.

  19. Pharmacokinetic profile of a locally administered doxycycline gel in crevicular fluid, blood, and saliva.

    Science.gov (United States)

    Kim, Ti-Sun; Bürklin, Thomas; Schacher, Beate; Ratka-Krüger, Petra; Schaecken, Matthijs T; Renggli, Heinz H; Fiehn, Walter; Eickholz, Peter

    2002-11-01

    With the help of so-called controlled release delivery systems, the half-life period of locally administered antibiotics in gingival crevicular fluid (GCF) can be extended significantly. The aim of this study was to characterize the delivery profile of a new one-component 14% doxycycline free amine gel for local application. Pharmacokinetics of doxycycline (DOXY) were analyzed in GCF, saliva, and serum. Twenty patients with persisting or recurring pockets (probing depths > or = 5 mm and bleeding on probing) after mechanical treatment (surgical or non-surgical) took part in the study. In each patient 1 periodontal defect was treated with DOXY gel. Samples of GCF, saliva, and serum were obtained before application of DOXY gel; 15 minutes after application; at 2 and 5 hours; and at 1, 2, 3, 4, 7, 9, and 11 days after application. Separation and quantitative measurement of DOXY was performed with high performance liquid chromatography and UV detection at lambda = 260 nm. Coefficients of variation were lower than 2% (intraassay) and 4% (interassay), respectively. For concentrations between 50 to 1000 microg/ml, we found a linear relationship between expected and measured DOXY values (linear coefficient of correlation: r = 0.998). Within the first 5 hours after application, concentration of DOXY in GCF (maximum after 15 minutes 19.97 +/- 5.85 mg/ml) and saliva (maximum after 15 minutes 17.83 +/- 2.84 mg/ml) was similar. Then concentration fell to a lower level (28.90 +/- 19.44 microg/ml) compared to GCF (577.41 +/- 127.34 microg/ml) after 3 days. Up to 10 days after application, the concentration of DOXY in GCF was 34.24 microg/ml. With the exception of 1 patient, all serum samples were DOXY-negative. 1) After subgingival application of biodegradable 14% doxycycline gel, mean doxycycline levels in GCF that exceeded 16 microg/ml could be maintained for at least 12 days. Thus, the antimicrobial agent may be classified as a controlled release device. 2) The antibiotic

  20. Immunohistological analysis of CD1a + langerhans cells and CD57 + natural killer cells in healthy and diseased human gingival tissue: A comparative study

    Directory of Open Access Journals (Sweden)

    Stelin Sahaya

    2009-01-01

    Full Text Available Background: Cell interaction between dendritic cells (DC and natural killer (NK cells in the periodontal milieu is not yet fully known, although these cells are individually known to contribute to the pathogenesis of periodontal disease. Materials and Methods: Fifty subjects (25 males and 25 females were included in the study. The subjects were divided into three groups: Group A comprised 16 subjects with clinically healthy gingiva; group B 17 subjects with gingivitis; and group C 17 subjects with gingivitis; and group C 17 subjects with moderate periodontitis (PPD ≥ 5 mm and CAL ≥ 3 mm in at least six sites. Gingival samples were collected and immunohistochemical study was done using CD57 and CD1a antibody. Statistical analysis was done using analysis of variance (ANOVA, followed by Tukey-Kramer multiple comparison for CD1a and Tukey′s highly significant difference (HSD test for CD57. Results and Conclusion: The study showed an inverse relationship between the CD1a+ (langerhans cells and CD57+ (natural killer cells. There was a significant increase in CD57+ cells and reduction in CD1a levels as periodontal disease progressed. The significant reduction in CD1a levels in periodontal disease when compared to health could possibly be a result of NK cells down regulating it. Reduction in CD1a levels may result in a low inflammatory response subsequently resulting in tissue destruction.

  1. Hydrogen-rich water achieves cytoprotection from oxidative stress injury in human gingival fibroblasts in culture or 3D-tissue equivalents, and wound-healing promotion, together with ROS-scavenging and relief from glutathione diminishment.

    Science.gov (United States)

    Xiao, Li; Miwa, Nobuhiko

    2017-04-01

    The aim of the present study is to investigate protective effects of hydrogen-rich water (HW) against reactive oxygen species (ROS)-induced cellular harmful events and cell death in human gingival fibroblasts (HGF) and three-dimensional (3D-) gingival tissue equivalents. HW was prepared with a magnesium stick in 600-mL double distilled water (DDW) overnight. Dissolved hydrogen was about 1460 ± 50 μg/L versus approximately 1600 μg/L for the saturated hydrogen. Under cell-free conditions, HW, dose-dependently, significantly scavenged peroxyl radicals (ROO·) derived from 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH). Extract from HW-treated HGF cells scavenged ROO· more markedly than that from DDW-treated cells, suggesting that HW can increase the intracellular antioxidant capacity. Hydrogen peroxide dose-dependently increased the intracellular ROS generation, which was significantly repressed by HW, both in the cytoplasm and nuclei. LIVE/DEAD staining and our original cell viability dye-extraction assay showed that HW significantly protected HGF cells from hydrogen peroxide-induced cell death. Hydrogen peroxide also diminished the contents of intracellular glutathione, which were appreciably relieved by HW-pretreatment. Additionally, HW noticeably prevented cumene hydroperoxide-induced generation of cellular ROS in epidermis parts of 3D-gingival equivalents. The in vitro scratch assay showed that HW was able to diminish physical injury-induced ROS generation and promote wound healing in HGF cell monolayer sheets. In summary, HW was able to increase intracellular antioxidative capacity and to protect cells and tissue from oxidative damage. Thus, HW might be used for prevention/treatment of oxidative stress-related diseases.

  2. RISIKO PEROKOK TERHADAP KEJADIAN GINGIVITIS

    Directory of Open Access Journals (Sweden)

    Niniek L. Pratiwi

    2012-11-01

    Full Text Available This study was done inorder to understand risk increasing coverage gingivitis at smokers. A crossectional study is conducted at Tambakrejo Surabaya Community Health Center. The sample of the study are patients that visit the Dental clinic of the Community Health Center, men aged between 18-45 years old and were not yet treated for scaling before. The number of sample are 191 respondents. The data analyzed by Chisquare For Trends. The result of this study showed that the risk of gingivitis among smokers increase as the quantity of cigarettes sucked per day increase.   Key  words: Smokers;Risk factor of Gingivitis

  3. Gingival plasma cell granuloma

    Directory of Open Access Journals (Sweden)

    Phadnaik Mangesh

    2010-01-01

    Full Text Available Plasma cell granuloma is a rare reactive lesion composed of polyclonal plasma cells. It manifests primarily in the lungs, but may occur in various other anatomic locations like the oral cavity. Intraoral plasma cell granulomas involving the tongue, lip, oral mucosa and gingiva have been reported in the past. This case presents a 54-year-old female with chronic periodontitis and mandibular anterior gingival overgrowth treated by Phase I therapy (scaling and root planing and excisional biopsy. Histological examination revealed inflammatory cell infiltrate containing sheets of plasma cells. Immunohistochemistry for kappa and lambda light chains showed a polyclonal staining pattern confirming a diagnosis of plasma cell granuloma. This case highlights the need to biopsy for unusual lesions to rule out potential neoplasms.

  4. Amlodipine-induced gingival overgrowth

    Directory of Open Access Journals (Sweden)

    Triveni M

    2009-01-01

    Full Text Available Gingival overgrowth represents an over-exuberant response to a variety of local and systemic conditions. Certain anticonvulsants, immuno-suppressive drugs and a number of calcium channel blockers have been shown to produce similar gingival overgrowths in certain susceptible patients. Amlodipine is a comparatively new calcium channel blocker and has been used with increasing frequency in the management of hypertension and angina. Although amlodipine is considered as a safe drug, very rarely it may induce gingival overgrowth also. A rare case of amlodipine-induced gingival overgrowth has been reported herein in a 50-year-old female patient. The treatment aspect included Phase-1 therapy, substitution of the drug, the surgical excision and the maintenance and supportive therapy resulting in excellent clinical outcome.

  5. Bir Olgu Nedeniyle Deskuamatif Gingivitis

    OpenAIRE

    Baloş, Köksal; ARPAK, Nejat

    2013-01-01

    ÖZETBu yazıda Deskuamatif gingivitis ve bu tanı konan hcsta gözden geçirilmiştir. Tedavi olarak gingivektomi işleminin etkinliği ortaya konmuştur.SUMMARYIn this article, desquamative gingivitis and an interesting a case have been reported. The effect of gingivectomy procedure has been demostrated.

  6. Gingivitis/stomatitis in cats.

    Science.gov (United States)

    Williams, C A; Aller, M S

    1992-11-01

    Any alteration in the balance of bacterial challenge versus the host's ability to resist and repair will result in oral lesions that are similar in appearance. The bacterial cause of gingivitis and periodontitis in humans and in all other animals in which it has been studied is firmly established, and specific species of predominantly gram-negative anaerobes have been implicated. Naturally occurring or acquired immunopathologies are likely to result in premature dental disease. When oral disease is associated with the accumulation of plaque, a positive response can be achieved by reducing the bacterial challenge to the host through the maintenance of oral hygiene by timely professional dental prophylaxis and home care. Disease that is the result of atypical immune responses, however, can be much more difficult to manage. Such oral disease can occur with either immune deficiencies or exaggerated immune responses, and it is likely that multiple mechanisms are active concurrently. In any case, gram-negative anaerobes present in plaque are likely to be a major contributing factor. Therefore patients with chronic refractory gingivitis-stomatitis must be considered to be plaque intolerant. Only with a frequent regimen of aggressive and thorough professional dental treatment plus meticulous oral home care on a daily basis can one expect to keep these cases in remission. Because this is often unrealistic, the only other way to keep these patients free of disease is by total dental extraction. The tissues that are colonized by the causative organisms must be eliminated. All root tips and bony sequestra must be removed and healing with intact epithelium accomplished before these cases will go into remission. Edentulous feline patients that continue to have signs of gingivostomatitis have been found to have an area of nonhealed bony sequestrum and chronic osteomyelitis. Once effective debridement has been accomplished and epithelial healing completed, nonresponsive cases can

  7. Disruption of the ECM33 Gene in Candida albicans Prevents Biofilm Formation, Engineered Human Oral Mucosa Tissue Damage and Gingival Cell Necrosis/Apoptosis

    Directory of Open Access Journals (Sweden)

    Mahmoud Rouabhia

    2012-01-01

    Full Text Available In this study we demonstrated that ΔCaecm33 double mutant showed reduced biofilm formation and causes less damage to gingival mucosa tissues. This was confirmed by the reduced level of necrotic cells and Bax/Bcl2 gene expression as apoptotic markers. In contrast, parental and Caecm33 mutant strains decreased basement membrane protein production (laminin 5 and type IV collagen. We thus propose that ECM33 gene/protein represents a novel target for the prevention and treatment of infections caused by Candida.

  8. Cetylpyridinium chloride mouth rinses alleviate experimental gingivitis by inhibiting dental plaque maturation

    Institute of Scientific and Technical Information of China (English)

    Fei Teng; Rui Li; Jun-Qi Ling; Tao He; Shi Huang; Cun-Pei Bo; Zhen Li; Jin-Lan Chang; Ji-Quan Liu; Duane Charbonneau; Jian Xu

    2016-01-01

    Oral rinses containing chemotherapeutic agents, such as cetylpyridinium chloride (CPC), can alleviate plaque-induced gingival infections, but how oral microbiota respond to these treatments in human population remains poorly understood. Via a double-blinded, randomised controlled trial of 91 subjects, the impact of CPC-containing oral rinses on supragingival plaque was investigated in experimental gingivitis, where the subjects, after a 21-day period of dental prophylaxis to achieve healthy gingivae, received either CPC rinses or water for 21 days. Within-subject temporal dynamics of plaque microbiota and symptoms of gingivitis were profiled via 16S ribosomal DNA gene pyrosequencing and assessment with the Mazza gingival index. Cetylpyridinium chloride conferred gingival benefits, as progression of gingival inflammation resulting from a lack of dental hygiene was significantly slower in the mouth rinse group than in the water group due to inhibition of 17 gingivitis-enriched bacterial genera. Tracking of plaqueα andβ diversity revealed that CPC treatment prevents acquisition of new taxa that would otherwise accumulate but maintains the original biodiversity of healthy plaques. Furthermore, CPC rinses reduced the size, local connectivity and microbiota-wide connectivity of the bacterial correlation network, particularly for nodes representing gingivitis-enriched taxa. The findings of this study provide mechanistic insights into the impact of oral rinses on the progression and maturation of dental plaque in the natural human population.

  9. Practice-based preclinical instruction for gingival displacement with animal models.

    Science.gov (United States)

    Cai, He; Yang, Shuying; Pei, Xibo; Qing, Hai; Wang, Jian

    2017-03-01

    Gingival displacement is recognized as a substantive and difficult procedure in fixed prosthodontics. However, a realistic simulation of gingival displacement is unavailable for preclinical dental students. The purpose of this study was to evaluate whether practice-based preclinical instruction of gingival displacement with animal models could improve students' skill in patient care. Isolated bovine mandibles (calves were younger than 6 months of age) and isolated porcine hemimandibles were prepared for this study. Twenty-two general dental practitioners with at least 5 years of experience were randomly selected and assigned to perform gingival displacement on both bovine and porcine jaws. Those practitioners were then asked to assess the clinical similarity of gingival displacement between human teeth and animal teeth. The data were analyzed with the paired t test (α=.05). Upon confirmation that the animal jaw provided a similar gingival displacement environment to that of human teeth, 80 predoctoral dental students were enrolled and randomized into 2 groups. Half of them underwent the new practice-based instruction, while the others underwent traditional preclinical teaching only (lectures, online video, or live demonstration). After preclinical learning, clinical performance in gingival displacement was evaluated for all students in terms of the effect of gingival displacement and quality of impression. The data were analyzed with the chi-square test (α=.05). The dentogingival environments of porcine and bovine jaws were similar to those of human jaws, and no significant difference was detected between these 2 animal models (P=.178). A significant increase occurred in the acceptable rate of the effect of gingival displacement (Pdisplacement with animal models is an effective method of promoting dental students' learning of gingival displacement. Copyright © 2016 Editorial Council for the Journal of Prosthetic Dentistry. Published by Elsevier Inc. All rights

  10. Gingival prosthesis: A treatment modality for recession

    Directory of Open Access Journals (Sweden)

    Pallavi Samatha Yalamanchili

    2013-01-01

    Full Text Available Gingival recession caused due to periodontal disease disturbs patients because of sensitivity and esthetics. Gingival prosthesis may be fixed or removable and can be made from silicones, acrylics, composite resins or ceramics according to what is best suited for the case. The gingival veneer is esthetically appealing and easy to maintain. This case report describes the use of gingival veneer as a treatment modality for recession.

  11. Gingival prosthesis: A treatment modality for recession

    OpenAIRE

    Pallavi Samatha Yalamanchili; Hemchand Surapaneni; Arunima Padmakumar Reshmarani

    2013-01-01

    Gingival recession caused due to periodontal disease disturbs patients because of sensitivity and esthetics. Gingival prosthesis may be fixed or removable and can be made from silicones, acrylics, composite resins or ceramics according to what is best suited for the case. The gingival veneer is esthetically appealing and easy to maintain. This case report describes the use of gingival veneer as a treatment modality for recession.

  12. Gingival enlargement in myelodysplastic syndrome

    Directory of Open Access Journals (Sweden)

    Navia George

    2015-01-01

    Full Text Available The myelodysplastic syndrome (MDS is characterized by peripheral blood cytopenias and increased risk of transformation to acute myeloid leukemia. This syndrome affects blood cell production and behavior. MDS is difficult to diagnose because of the absence of symptoms in the early stage of the disease. Often it is accidentally discovered during a routine physical exam/blood test. Till date, only a few cases of gingival enlargement associated with MDS are reported in the literature. Here is a remarkable case of gingival enlargement heralding the presence of MDS.

  13. Gingival and periodontal ligament fibroblasts differ in their inflammatory response to viable Porphyromonas gingivalis

    NARCIS (Netherlands)

    Scheres, N; Laine, M L; de Vries, T J; Everts, V; van Winkelhoff, A J

    2010-01-01

    BACKGROUND AND OBJECTIVE: Porphyromonas gingivalis is an oral pathogen strongly associated with destruction of the tooth-supporting tissues in human periodontitis. Gingival fibroblasts (GF) and periodontal ligament fibroblasts (PDLF) are functionally different cell types in the periodontium that can

  14. [A case of gingival myiasis caused by Wohlfahrtia magnifica].

    Science.gov (United States)

    Çetın Özdemır, Eda; Ekşi, Fahriye; Şenyurt, Süleyman Ziya; Üstün, Kemal; Karaoğlan, İlkay; Ercıyas, Kamile

    2014-07-01

    Myiasis is an infestation of living or dead tissue of humans and animals by diptera larvae. Gingival myiasis is a rare pathology and is mainly associated with poor oral hygiene, alcoholism, senility, suppurative lesions, mouth breathing, mental retardation and hemiplegia. Myiasis is most common during summer since the fly population increases during this season. Mostly it occurs in farmers and people who live in tropical climates. Gingival myiasis in humans in Turkey is limited to only a few cases. According to our literature research, this is the first case of gingival myiasis produced by larvae of Wohlfahrtia magnifica in a Turkish adult. According to our best knowledge, it is also the first gingival myiasis case that one of the causative larva had grown to the adult stage in Turkey. A 43 years old male patient who perceived the presence of live maggots in his mouth was referred to our clinic. Clinical findings of gingival myiasis were observed. The patient had no history of systemic disease but oral hygiene was poor. Clinical and radiographic examination indicated that he had chronic periodontitis. Before the dental treatment seven larvae and during the scalling five larvae were elevated from the gingival sulcus. The body of the larvae composed of 12 segments and they were 8-10 mm in length. One of the larvae which was sent to the microbiology laboratory were placed into sheep liver to resume life and the other larvae were placed into 70% alcohol solution. After 9-10 days, the larva which was placed in the liver became pupa. Approximately 15 days later, the pupa became an adult fly. The larvae were identified as the second stage larvae of Wohlfahrtia magnifica. Treatment consisted of removal of the maggots from the gingival sulcus, followed by scaling and oral hygiene instruction. Non-surgical periodontal treatment was applied and the patient was followed-up for 3 months. After non-surgical periodontal treatment, patient didn't accept the flap operation. The

  15. Interlinking Periodontitis and Type 2 Diabetes Mellitus by Assessment of Crevicular Visfatin Levels in Health and in Disease Before and After Initial Periodontal Therapy.

    Science.gov (United States)

    Mishra, Vandita; Shettar, Leena; Bajaj, Mahesh; Math, Abhishek Savir; Thakur, Srinath L

    2016-08-01

    Visfatin is a new adipocytokine associated with both chronic periodontitis and type 2 diabetes mellitus independently. We aimed to estimate and compare the changes in the levels of visfatin in the Gingival Crevicular Fluid (GCF) of healthy subjects and in subjects with periodontitis with or without controlled Type 2 Diabetes Mellitus (T2DM) after administration of non-surgical periodontal therapy. Forty two subjects were equally divided into Group 1 (healthy), Group 2 (systemically healthy with chronic periodontitis), Group 3 (subjects with chronic periodontitis having controlled T2DM). Defined clinical parameters were recorded at baseline and at one month follow-up period. Visfatin was assessed using enzyme linked immunosorbent assay. One way ANOVA and Tukey's multiple post hoc procedures were used. Pearson's correlation coefficient was used for correlation. Significant increase in the visfatin levels was seen with the highest values observed in diabetes with periodontal disease. Visfatin responded to non-surgical periodontal therapy as observed by significant decrease in levels after one month but even at this period diabetics showed the highest levels. Visfatin levels are highest in individuals with both periodontal disease and diabetes even after periodontal therapy. Individuals with T2DM may be at higher risk of developing periodontal disease.

  16. A rare clinical presentation of sarcoidosis; gingivitis.

    Science.gov (United States)

    Güzel, Aygül; Köksal, Nurhan; Aydın, Davut; Aslan, Kerim; Gören, Fikret; Karagöz, Filiz

    2013-10-01

    Gingivitis due to sarcoidosis is a relatively rare condition. Gingivitis or isolated gingival involvement may be the first sign of systemic sarcoidosis. We report the case of a 37 year-old woman with isolated gingivitis due to sarcoidosis confirmed by biopsy. Following treatment with a systemic corticosteroid (prednisolone 40 mg/day), all clinical and radiologic findings were completely improved. In cases of chronic and intractable gingivitis, systemic sarcoidosis should be suspected. It should be confirmed with a biopsy, and the patient should be referred to a chest disease clinic to exclude other organ involvement.

  17. Pharm GKB: Acute ulcerative gingivitis [PharmGKB

    Lifescience Database Archive (English)

    Full Text Available Overview Alternate Names: Synonym ANUG - Acute necrotising ulcerative gingivitis; ANUG - Acute... necrotizing ulcerative gingivitis; AUG - Acute ulcerative gingivitis; Vincent's disease; Vincent...g] (N0000001343) Common Searches Search Medline Plus Search CTD Pharm GKB: Acute ulcerative gingivitis ... ...ive (D005892) SnoMedCT: Acute ulcerative gingivitis (172697005) UMLS: C0017575 (C...'s gingivitis PharmGKB Accession Id: PA165109110 External Vocabularies MeSH: Gingivitis, Necrotizing Ulcerat

  18. Non-plaque-induced gingival lesions

    DEFF Research Database (Denmark)

    Holmstrup, P

    1999-01-01

    The origin of gingival inflammation is occasionally different from that of routine plaque-associated gingivitis, and such non-plaque-associated types of gingivitis often present characteristic clinical features. Examples of such forms of gingivitis are specific bacterial, viral, and fungal......, a confirmed diagnosis may require histopathologic examination and/or culture. Atypical gingivitis may also occur as gingival manifestations of dermatological diseases, the most relevant of these being lichen planus, pemphigoid, pemphigus vulgaris, erythema multiforme, and lupus erythematosus. Non-plaque......, the most important of these being Candida species including C. albicans, C. glabrata, C. krusei, C. tropicalis, C. parapsilosis, and C. guillermondii. Gingival histoplasmosis is a granulomatous disease caused by the fungus Histoplasma capsulatum and, as for the other specific infections of gingiva...

  19. Nonsurgical Management of Nifedipine Induced Gingival Overgrowth

    Directory of Open Access Journals (Sweden)

    George Sam

    2014-01-01

    Full Text Available Drug-induced gingival overgrowth is frequently associated with three particular drugs: phenytoin, cyclosporin, and nifedipine. As gingival enlargement develops, it affects the normal oral hygiene practice and may interfere with masticatory functions. The awareness in the medical community about this possible side effect of nifedipine is less when compared to the effects of phenytoin and cyclosporin. The frequency of gingival enlargement associated with chronic nifedipine therapy remains controversial. Within the group of patients that develop this unwanted effect, there appears to be variability in the extent and severity of the gingival changes. Although gingival inflammation is considered a primary requisite in their development, few cases with minimal or no plaque induced gingival inflammation have also been reported. A case report of gingival overgrowth induced by nifedipine in a patient with good oral hygiene and its nonsurgical management with drug substitution is discussed in this case report.

  20. Potential Immune Modularly Role of Glycine in Oral Gingival Inflammation

    Directory of Open Access Journals (Sweden)

    Teresa Schaumann

    2013-01-01

    Full Text Available Gingival epithelial cells (GECs represent a physical barrier against bacteria and are involved in the processes of innate immunity. Recently, an anti-inflammatory and immune-modulatory effect of the amino acid glycine has been demonstrated. However, there is only little information about the immune-modulatory effects of glycine in oral tissues. This study aimed to investigate the existence and role of the glycine receptor in gingival tissue analyzing tissues/cells from extracted human molars via immunohistochemical analysis. In vitro, GECs were challenged by inflammatory conditions with IL-1β alone or in combination with glycine and analyzed for cytokine expression of IL6/IL8 via real-time PCR. On protein level, the effect of nuclear translocalization of NFκB protein p65 was analyzed using immunofluorescence analysis. A distinct proof of the GlyR in oral gingival tissue and keratinocytes could be demonstrated. Isolated challenge of the keratinocytes with IL-1β as well as with glycine resulted in an upregulation of IL6 and IL8 mRNA expression and activation of NFκB pathway. The presence of glycine in combination with the inflammatory stimulus led to a significant decrease in inflammatory parameters. These results indicate a possible anti-inflammatory role of glycine in gingival inflammation and encourage further research on the utility of glycine in the prevention or therapy of inflammatory periodontitis.

  1. Effect of phenytoin and age on gingival fibroblast enzymes.

    Directory of Open Access Journals (Sweden)

    Surena Vahabi

    2014-06-01

    Full Text Available The alteration of cytokine balance is stated to exert greater influence on gingival overgrowth compared to the direct effect of the drug on the regulation of extracellular matrix metabolism. The current study evaluated the effect of phenytoin on the regulation of collagen, lysyl oxidase and elastin in gingival fibroblasts.Normal human gingival fibroblasts (HGFs were obtained from 4 healthy children and 4 adults. Samples were cultured with phenytoin. MTT test was used to evaluate the proliferation and ELISA was performed to determine the level of IL1β and PGE2 production by HGFs. Total RNA of gingival fibroblasts was extracted and RT-PCR was performed on samples. Mann-Whitney U test was used to analyze the data with an alpha error level less than 0.05.There was a significant difference in the expression of elastin between the controls and treated samples in both adult and pediatric groups and also in the lysyl oxidase expression of adult controls and treated adults. No significant difference was found between collagen expression in adults.The significant difference in elastin and lysyl oxidase expression between adult and pediatric samples indicates the significant effect of age on their production.

  2. Does gingival recession require surgical treatment?

    Science.gov (United States)

    Chan, Hsun-Liang; Chun, Yong-Hee Patricia; MacEachern, Mark

    2016-01-01

    Gingival recession represents a clinical condition in adults frequently encountered in the general dental practice. It is estimated that 23% of adults in the US have one or more tooth surfaces with ≥ 3 mm gingival recession. Clinicians often time face dilemmas of whether or not to treat such a condition surgically. Therefore, we were charged by the editorial board to answer this critical question: “Does gingival recession require surgical treatment?” An initial condensed literature search was performed using a combination of gingival recession and surgery controlled terms and keywords. An analysis of the search results highlights our limited understanding of the factors that often guide the treatment of gingival recession. Understanding the etiology, prognosis and treatment of gingival recession continues to offer many unanswered questions and challenges in the field of periodontics as we strive to provide the best care possible for our patients. PMID:26427577

  3. Idiopathic gingival enlargement and its management

    Directory of Open Access Journals (Sweden)

    Shetty Arvind

    2010-01-01

    Full Text Available Idiopathic gingival enlargement is a proliferative fibrous lesion of the gingival tissue that causes esthetic and functional problems. Both genetically and pharmacologically induced forms of gingival enlargement exist. This case report addresses the diagnosis and treatment of a case of idiopathic gingival enlargement in a 13-year-old female. The patient presented with generalized diffuse gingival enlargement involving the maxillary and mandibular arches extending on buccal and lingual/palatal surfaces and covering incisal / occlusal third of the tooth resulting in difficulty in speech and mastication since last three years. Patient also gave a history of surgical treatment being carried out four years back in upper anterior region suggesting of recurrence. Biopsy report confirmed the diagnosis of gingival hyperplasia. Gingivectomy was carried out in all four quadrants by using four different methods.

  4. Nonsurgical gingival displacement in restorative dentistry.

    Science.gov (United States)

    Thomas, Manuel S; Joseph, Robin Mathai; Parolia, Abhishek

    2011-06-01

    Gingival displacement is critical for obtaining accurate impressions for the fabrication of fixed restorations, especially when the finish line is at or just within the gingival sulcus. Displacement of the gingival tissue is also important when dealing with the restoration of cervical lesions due to their proximity to the periodontal tissue. The methods of gingival tissue displacement can be broadly classified as nonsurgical and surgical techniques, with nonsurgical being the more commonly practiced method. Dentists must alter their armamentarium and gingival displacement techniques to meet specific demands and obtain predictable results. Hence, the purpose of this article is to describe the different means by which nonsurgical gingival displacement can be achieved effectively under a variety of clinical situations.

  5. Nifedipine-Induced gingival overgrowth

    OpenAIRE

    Florio, Ornella; School of Dentistry of São Jose dos Campos - State University of São Paulo; Tfouni, Maysa; School of Dentistry of São Jose dos Campos - State University of São Paulo; Balducci, Ivan; School of Dentistry of São Jose dos Campos - State University of São Paulo; Marco, Andrea Carvalho de; School of Dentistry of São Jose dos Campos - State University of São Paulo; Neves Jardini, Maria Aparecida; School of Dentistry of São Jose dos Campos - State University of São Paulo; Dias Almeida, Janete; School of Dentistry of São Jose dos Campos - State University of São Paulo

    2013-01-01

    Objective: The objective of the present study was to evaluate gingival overgrowth induced by nifedipine and to correlate it with plaque accumulation. Material and Methods: Sixty patients were divided into a treated group (n=30) consisting of hypertensive patients treated with nifedipine and a control group (n=30) consisting of patients without arterial hypertension. The following exams were performed on the first visit: anamnesis, measurement of blood pressure, weight and height, extra- and i...

  6. Effection of enamel matrix proteins on proliferation and Type I collagen synthesis of human gingival mesenchymal stem cells%EMP对人牙龈间充质干细胞增殖和Ⅰ型胶原合成的影响

    Institute of Scientific and Technical Information of China (English)

    常秀梅; 张克荣; 蔡洁琛; 齐香薇; 王书文

    2015-01-01

    Objective To assess the effects of enamel matrix proteins on proliferation and type I colla⁃gen synthesis of human gingival mesenchymal stem cells(hGMSCs). Methods The primary gingival fi⁃broblasts were cultured and then mesenchymal stem cell surface markers STRO-1 were characterized by Immunocytochemistry.hGMSCs was cultured in concentrations of enamel matrix protein 25 mg/L (A1 group),50 mg/L (A2 group),100 mg/L (A3 group),200 mg/L (groupA4),control group (group A0) DMEM solution of 2%FCS training,The cell proliferation was detected by MTT. Immunocytochemistry and image analysis of type I collagen synthesis. Results 50-200mg/L showed hGMSCs proliferation promoting effect,50 mg/L EMPs that can significantly promote the growth of hGMSCs,but to promote proliferation of the strongest 100 mg/L EMPs.(Compared with other groups,P<0.01) in experimental group was better than control group,collagen synthesis.Among them,the most obvious 100mg/L pro⁃mote the synthesis of collagen type I,and changing with time. Conclusion The enamel matrix protein can promote the proliferation of hGMSCs and type I collagen synthesis.%目的:观察釉基质蛋白(enamel matrix proteins,EMPs)对牙龈间充质干细胞(human gingival mesenchymal stem cells,hGMSCs)增殖和Ⅰ型胶原合成的影响。方法:分离培养人牙龈成纤维细胞,免疫细胞化学方法检测其间充质干细胞特性STRO-1的表达,不同浓度EMPs,25 mg/L(A1组)、50 mg/L(A2组)、100 mg/L(A3组)、200 mg/L(A4组),对照组(A0组)10%FCS的DMEM溶液培养,MTT检测EMPs对细胞增殖活性的影响。免疫细胞化学方法鉴定细胞Ⅰ型胶原合成和图像分析。结果:50~200mg/L表现出促hGMSCs增殖的作用,50 mg/L EMPs即可以显著促进hGMSCs的生长,但以100 mg/L EMPs的促增殖作用最强(与其余各组相比P<0.01),实验组胶原合成明显强于对照组。其中,100mg/L促 I型胶原(collagen typeⅠ,COLⅠ—合成

  7. The pro-apoptotic and pro-inflammatory effects of calprotectin on human periodontal ligament cells.

    Directory of Open Access Journals (Sweden)

    Yunfei Zheng

    Full Text Available Calprotectin, a heterodimer of S100A8 and S100A9 subunits, is associated with inflammatory disorders such as rheumatoid arthritis and cystic fibrosis. Although calprotectin levels are increased significantly in the gingival crevicular fluid (GCF of periodontitis patients, its effects on periodontal ligament cells (PDLCs remain largely unknown. The aim of this study was to evaluate calprotectin levels in the GCF of generalized aggressive periodontitis (AgP patients and to investigate the effects of recombinant human calprotectin (rhS100A8/A9 and its subunits (rhS100A8 and rhS100A9 in PDLCs. Both the concentration and amount of crevicular calprotectin were significantly higher in the AgP group compared with healthy controls. In addition, the GCF calprotectin levels were correlated positively with clinical periodontal parameters including bleeding index, probing depth, and clinical attachment loss. rhS100A8/A9 promoted cell apoptosis, whereas rhS100A8 and rhS100A9 individually exerted little effect on apoptosis in PDLCs. rhS100A9 and rhS100A8/A9 increased the activation of nuclear factor-κB (NF-κB by promoting the nuclear translocation of p65 in PDLCs, subsequently inducing expression of the pro-inflammatory cytokines IL-6, IL-8, TNFα, and COX2. Treatment with an NF-κB inhibitor partially reversed the rhS100A9- and rhS100A8/A9-induced upregulation of the pro-inflammatory cytokines. rhS100A9, and not rhS100A8, was mainly responsible for the pro-inflammatory role of calprotectin. Collectively, our results suggest that calprotectin promotes apoptosis and the inflammatory response in PDLCs via rhS100A9. These findings might help identify novel treatments for periodontitis.

  8. The pro-apoptotic and pro-inflammatory effects of calprotectin on human periodontal ligament cells.

    Science.gov (United States)

    Zheng, Yunfei; Hou, Jianxia; Peng, Lei; Zhang, Xin; Jia, Lingfei; Wang, Xian'e; Wei, Shicheng; Meng, Huanxin

    2014-01-01

    Calprotectin, a heterodimer of S100A8 and S100A9 subunits, is associated with inflammatory disorders such as rheumatoid arthritis and cystic fibrosis. Although calprotectin levels are increased significantly in the gingival crevicular fluid (GCF) of periodontitis patients, its effects on periodontal ligament cells (PDLCs) remain largely unknown. The aim of this study was to evaluate calprotectin levels in the GCF of generalized aggressive periodontitis (AgP) patients and to investigate the effects of recombinant human calprotectin (rhS100A8/A9) and its subunits (rhS100A8 and rhS100A9) in PDLCs. Both the concentration and amount of crevicular calprotectin were significantly higher in the AgP group compared with healthy controls. In addition, the GCF calprotectin levels were correlated positively with clinical periodontal parameters including bleeding index, probing depth, and clinical attachment loss. rhS100A8/A9 promoted cell apoptosis, whereas rhS100A8 and rhS100A9 individually exerted little effect on apoptosis in PDLCs. rhS100A9 and rhS100A8/A9 increased the activation of nuclear factor-κB (NF-κB) by promoting the nuclear translocation of p65 in PDLCs, subsequently inducing expression of the pro-inflammatory cytokines IL-6, IL-8, TNFα, and COX2. Treatment with an NF-κB inhibitor partially reversed the rhS100A9- and rhS100A8/A9-induced upregulation of the pro-inflammatory cytokines. rhS100A9, and not rhS100A8, was mainly responsible for the pro-inflammatory role of calprotectin. Collectively, our results suggest that calprotectin promotes apoptosis and the inflammatory response in PDLCs via rhS100A9. These findings might help identify novel treatments for periodontitis.

  9. [Gingival bleaching: teaching and ethnocentrism].

    Science.gov (United States)

    Bolla, Edson Daruich; Goldenberg, Paulete

    2010-06-01

    The aim of this study was to identify buccal/gingival cosmetic dentistry patterns subjacent to formation and professional practice of the dental surgeon from the ethnocentrism point of view. This is an exploratory study with a qualitative approach based on the thematic analysis. Initially a documental analysis was carried out. Thereafter, dental surgeons were interviewed and semi-structured questions were applied. In the Periodontal teaching field, this study showed that the presence of racial melanosis is omitted or treated as an alteration in the normality patterns and it is considered anti-aesthetic. All the interviewers learnt how to practice gingival bleaching in the post-graduation courses, they were all encouraged to offer this cosmetic dentistry procedure with the opportunity of obtaining a beautiful and healthy smile, thus assuring the belief of the Caucasian racial aesthetic superiority. This study make us think that the offer of gingival bleaching is oriented by the Caucasian pattern of beauty evidencing the ethnocentric character of this procedure.

  10. Does gingival recession require surgical treatment?

    OpenAIRE

    Chan, Hsun-Liang; Chun, Yong-Hee Patricia; MacEachern, Mark

    2015-01-01

    Gingival recession represents a clinical condition in adults frequently encountered in the general dental practice. It is estimated that 23% of adults in the US have one or more tooth surfaces with ≥ 3 mm gingival recession. Clinicians often time face dilemmas of whether or not to treat such a condition surgically. Therefore, we were charged by the editorial board to answer this critical question: “Does gingival recession require surgical treatment?” An initial condensed literature search was...

  11. Unusual Gingival Enlargement: A Rare Case Report

    Directory of Open Access Journals (Sweden)

    Ashutosh Dixit

    2014-01-01

    Full Text Available This is an atypical case report of a 20-year-old male patient who suffered from unusual unilateral, gingival enlargement together with rapidly progressive alveolar bone loss. The enlarged gingiva completely covered his left posterior teeth in both arches. The patient was diagnosed with gingival fibromatosis and aggressive periodontitis based on the clinical, histological, and radiographic findings. The gingival enlargement was treated by conventional gingivectomy under local anaesthesia. The postoperative result was uneventful.

  12. Tapered toothbrush filaments in relation to gingival abrasion, removal of plaque and treatment of gingivitis

    NARCIS (Netherlands)

    Versteeg, P.A.; Piscaer, M.; Rosema, N.A.M.; Timmerman, M.F.; van der Velden, U.; van der Weijden, G.A.

    2008-01-01

    Objectives: To compare a tapered filament toothbrush (TFTB) to a control toothbrush (ADA) in their potential to cause gingival abrasion and improve the gingival condition following a period of experimental gingivitis. Methods: Thirty-two subjects refrained from brushing mandibular teeth for 21 days.

  13. 硝苯地平对人牙龈上皮细胞bcl-2基因表达的影响%Nifedipine regulated expression of bcl-2 in human gingival epithelial cells in vitro

    Institute of Scientific and Technical Information of China (English)

    文海燕; 束蓉; 蒋少云; 姜云涛

    2010-01-01

    目的:体外观察硝苯地平(nifedipine,NIF)对人牙龈上皮细胞(human gingival epithelial cells,HGECs)bcl-2基因转录水平的调节,探讨NIF诱导的药物性牙龈增生(drug-induced gingival overgrowth,DGO)与凋亡抑制基因bcl-2的相关性.方法:采用牙周手术切除的健康牙龈组织.用酶消化法分离培养HGECs;免疫组织化学方法对培养细胞进行细胞鉴定;实时定量PCR技术检测不同浓度NIF(1 μg/ml、2 μg/ml和3 μg/ml)刺激下HGECs中bcl-2 mRNA水平,以0 μg/ml NIF为空白对照.采用SPSS 11.0软件包对所得数据进行单因素方差分析.结果:酶消化法获得的HGECs在体外培养中生长状态良好;免疫组织化学显示,HGECs抗角蛋白染色阳性,抗波形蛋白染色阴性;NIF处理24h后的HGECs bcl-2 mRNA水平随NIF浓度的增高而上升,3 μg/ml浓度组与空白对照组有显著差异(P<0.05);NIF处理48h后.2 μg/ml、3 μg/ml浓度组HGECs bcl-2 mRNA水平与空白对照组差异明显(P<0.05).结论:NIF调节体外培养的HGECs中bcl-2基因转录的水平.

  14. Amelogenesis Imperfecta and Generalized Gingival Overgrowth Resembling Hereditary Gingival Fibromatosis in Siblings: A Case Report

    Directory of Open Access Journals (Sweden)

    Emre Yaprak

    2012-01-01

    Full Text Available Amelogenesis imperfecta (AI is a group of hereditary disorders primarily characterized by developmental abnormalities in the quantity and/or quality of enamel. There are some reports suggesting an association between AI and generalized gingival enlargement. This paper describes the clinical findings and oral management of two siblings presenting both AI and hereditary gingival fibromatosis (HGF like generalized gingival enlargements. The treatment of gingival enlargements by periodontal flap surgery was successful in the management of the physiologic gingival form for both patients in the 3-year follow-up period. Prosthetic treatment was also satisfactory for the older patient both aesthetically and functionally.

  15. Probing around implants and teeth with healthy or inflamed peri-implant mucosa/gingival. A histologic comparison in cynomolgus monkeys. (Macaca fascicularis)

    DEFF Research Database (Denmark)

    Schou, Søren; Holmstrup, Palle; Stoltze, K.

    2002-01-01

    Osseointegrated oral implants; teeth; phathology; peri-implant mucositis; gingivitis; peri-implantitis; periodontitis; diagnosis; probing depth; non-human primates; cynomolgus monkeys: Macaca fascicularis......Osseointegrated oral implants; teeth; phathology; peri-implant mucositis; gingivitis; peri-implantitis; periodontitis; diagnosis; probing depth; non-human primates; cynomolgus monkeys: Macaca fascicularis...

  16. Rat gingival model for testing drugs influencing inflammation

    Directory of Open Access Journals (Sweden)

    Shaju P Jacob

    2013-07-01

    Full Text Available Preclinical drug testing is an important areain new drug development where animals are used.An ideal animal model for this is one which is simple,reliable and can be extrapolated to humans. Topicaldrugs for inflammation are conventionally tested onthe skin of animals after induction of inflammation.A gingival model would be simple as inflammation canbe induced naturally by the action of plaque. Rats area popular animal model for testing drugs as well as tostudy various diseases of the periodontium. Periodontaldisease including gingival inflammation develops inrats in relation to indigenous plaque or experimentallyinduced bacterial products. A number of features ofrats ranging from anatomy, histology and response tobacterial insult can be seen mirrored to a great extentin humans. There is a lot similarity in the developmentand resolution of inflammation as well as the gingivalwound healing of rats and humans. This paper tries toexplore the feasibility of using the rat gingival modelfor preclinical testing of drugs acting on or influencinginflammation and concludes by identifying potentialareas of research using this model. The addition of sucha simple and inexpensive model for preclinical testing ofdrugs will be welcomed by the drug developers.

  17. [Periodontology and esthetics: the gingival recession].

    Science.gov (United States)

    Corba, N H

    1991-06-01

    Gingival recessions are regarded by many people as an esthetical problem. Successively the etiology, the significance and the indications for therapy are discussed. Different kinds of therapy such as oral hygiene instruction, the free gingival graft and various pedicle grafts are explained. Finally it is advocated that surgical kinds of therapy have to be applied with reservedness.

  18. Feline gingivitis-stomatitis-pharyngitis.

    Science.gov (United States)

    Diehl, K; Rosychuk, R A

    1993-01-01

    Inflammatory conditions of the feline mouth are commonly encountered in small animal practice. Although the majority can be attributed to dental disease and a small percentage are due to autoimmune diseases, the eosinophilic granuloma complex, neoplasia, and other miscellaneous syndromes, many cases appear to be due to a gingivitis-stomatitis-pharyngitis complex, which is likely multifactorial in origin. Viruses, bacterial infection, diet, dental disease, oral conformation, genetic predisposition, hypersensitivities, immunoinsufficiencies, and other defects in oral defense mechanisms may all be contributory. The complexities of this syndrome have made it one of the most challenging diagnostic and therapeutic problems in feline medicine.

  19. Preliminary characterization of the oral microbiota of Chinese adults with and without gingivitis

    Directory of Open Access Journals (Sweden)

    Huang Shi

    2011-12-01

    Full Text Available Abstract Background Microbial communities inhabiting human mouth are associated with oral health and disease. Previous studies have indicated the general prevalence of adult gingivitis in China to be high. The aim of this study was to characterize in depth the oral microbiota of Chinese adults with or without gingivitis, by defining the microbial phylogenetic diversity and community-structure using highly paralleled pyrosequencing. Methods Six non-smoking Chinese, three with and three without gingivitis (age range 21-39 years, 4 females and 2 males were enrolled in the present cross-sectional study. Gingival parameters of inflammation and bleeding on probing were characterized by a clinician using the Mazza Gingival Index (MGI. Plaque (sampled separately from four different oral sites and salivary samples were obtained from each subject. Sequences and relative abundance of the bacterial 16 S rDNA PCR-amplicons were determined via pyrosequencing that produced 400 bp-long reads. The sequence data were analyzed via a computational pipeline customized for human oral microbiome analyses. Furthermore, the relative abundances of selected microbial groups were validated using quantitative PCR. Results The oral microbiomes from gingivitis and healthy subjects could be distinguished based on the distinct community structures of plaque microbiomes, but not the salivary microbiomes. Contributions of community members to community structure divergence were statistically accessed at the phylum, genus and species-like levels. Eight predominant taxa were found associated with gingivitis: TM7, Leptotrichia, Selenomonas, Streptococcus, Veillonella, Prevotella, Lautropia, and Haemophilus. Furthermore, 98 species-level OTUs were identified to be gingivitis-associated, which provided microbial features of gingivitis at a species resolution. Finally, for the two selected genera Streptococcus and Fusobacterium, Real-Time PCR based quantification of relative bacterial

  20. The vaginal microflora in relation to gingivitis

    Directory of Open Access Journals (Sweden)

    Weibel Marianne

    2009-01-01

    Full Text Available Abstract Background Gingivitis has been linked to adverse pregnancy outcome (APO. Bacterial vaginosis (BV has been associated with APO. We assessed if bacterial counts in BV is associated with gingivitis suggesting a systemic infectious susceptibilty. Methods Vaginal samples were collected from 180 women (mean age 29.4 years, SD ± 6.8, range: 18 to 46, and at least six months after delivery, and assessed by semi-quantitative DNA-DNA checkerboard hybridization assay (74 bacterial species. BV was defined by Gram stain (Nugent criteria. Gingivitis was defined as bleeding on probing at ≥ 20% of tooth sites. Results A Nugent score of 0–3 (normal vaginal microflora was found in 83 women (46.1%, and a score of > 7 (BV in 49 women (27.2%. Gingivitis was diagnosed in 114 women (63.3%. Women with a diagnosis of BV were more likely to have gingivitis (p = 0.01. Independent of gingival conditions, vaginal bacterial counts were higher (p Prevotella bivia (p Prevotella disiens (p P. bivia, P. disiens, M. curtisii and M. mulieris (all at the p 1.0 × 104 cells and a diagnosis of gingivitis was 3.9 for P. bivia (95% CI 1.5–5.7, p P. disiens (95%CI: 1.8–7.5, p P. bivia (odds ratio: 5.3, 95%CI: 2.6 to 10.4, p P. disiens (odds ratio: 4.4, 95% CI: 2.2 to 8.8, p Conclusion Higher vaginal bacterial counts can be found in women with BV and gingivitis in comparison to women with BV but not gingivitis. P. bivia and P. disiens may be of specific significance in a relationship between vaginal and gingival infections.

  1. Inorganic chemistry of defensive peroxidases in the human oral cavity.

    Science.gov (United States)

    Ashby, M T

    2008-10-01

    The innate host response system is comprised of various mechanisms for orchestrating host response to microbial infection of the oral cavity. The heterogeneity of the oral cavity and the associated microenvironments that are produced give rise to different chemistries that affect the innate defense system. One focus of this review is on how these spatial differences influence the two major defensive peroxidases of the oral cavity, salivary peroxidase (SPO) and myeloperoxidase (MPO). With hydrogen peroxide (H(2)O(2)) as an oxidant, the defensive peroxidases use inorganic ions to produce antimicrobials that are generally more effective than H(2)O(2) itself. The concentrations of the inorganic substrates are different in saliva vs. gingival crevicular fluid (GCF). Thus, in the supragingival regime, SPO and MPO work in unison for the exclusive production of hypothiocyanite (OSCN(-), a reactive inorganic species), which constantly bathes nascent plaques. In contrast, MPO is introduced to the GCF during inflammatory response, and in that environment it is capable of producing hypochlorite (OCl(-)), a chemically more powerful oxidant that is implicated in host tissue damage. A second focus of this review is on inter-person variation that may contribute to different peroxidase function. Many of these differences are attributed to dietary or smoking practices that alter the concentrations of relevant inorganic species in the oral cavity (e.g.: fluoride, F(-); cyanide, CN(-); cyanate, OCN(-); thiocyanate, SCN(-); and nitrate, NO(3)(-)). Because of the complexity of the host and microflora biology and the associated chemistry, it is difficult to establish the significance of the human peroxidase systems during the pathogenesis of oral diseases. The problem is particularly complex with respect to the gingival sulcus and periodontal pockets (where the very different defensive stratagems of GCF and saliva co-mingle). Despite this complexity, intriguing in vitro and in vivo

  2. Melanin: A scavenger in gingival inflammation

    Directory of Open Access Journals (Sweden)

    S Nilima

    2011-01-01

    Full Text Available Background: One of the major direct or indirect targets of ultraviolet exposure of skin is the melanocyte or the melanin -forming cell. Epidermal melanocytes act as a trap for free radicals. Based on the protective role of melanocytes in medical literature, the role of melanin pigmentation in gingiva needs to be elucidated. Periodontal pathogens and their products demonstrate the ability to induce the generation of reactive oxygen species. Hence purpose of this study was to unravel the protective role of melanin (if any against the gingival inflammation. Materials and Methods: A total of 80 subjects; 20 in each group were selected. The selection of subjects regarding gingival pigmentation was based on Dummett′s scoring criteria 0, 3. A complete medical, dental history and an informed consent were obtained from the patients. After evaluation of clinical parameters the GCF was collected using microcapillary pipettes at the selected sites. IL-1β levels were quantitated using ELISA. Results: In non-pigmented healthy and gingivitis groups, there was a positive correlation between plaque index, gingival index and bleeding index versus IL-1β level: indicating an increase in the biochemical mediator of inflammation corresponding to an increase in the clinical parameters of inflammation. Also a positive correlation was found between the gingival index and bleeding index versus the IL-1β levels in the pigmented healthy group. The pigmented gingivitis groups showed a negative correlation between the plaque index, gingival index and bleeding index. Conclusions: The clinical markers of inflammation such as gingival index, bleeding index was of low numerical value in pigmented group than in the non-pigmented group, supposedly due to the protective action of melanin. The negative correlation of clinical markers of inflammation to the IL-1β levels in the pigmented gingivitis group could possibly be attributed to the protective role of melanins.

  3. Bacterial community development in experimental gingivitis.

    Science.gov (United States)

    Kistler, James O; Booth, Veronica; Bradshaw, David J; Wade, William G

    2013-01-01

    Current knowledge of the microbial composition of dental plaque in early gingivitis is based largely on microscopy and cultural methods, which do not provide a comprehensive description of oral microbial communities. This study used 454-pyrosequencing of the V1-V3 region of 16S rRNA genes (approximately 500 bp), and bacterial culture, to characterize the composition of plaque during the transition from periodontal health to gingivitis. A total of 20 healthy volunteers abstained from oral hygiene for two weeks, allowing plaque to accumulate and gingivitis to develop. Plaque samples were analyzed at baseline, and after one and two weeks. In addition, plaque samples from 20 chronic periodontitis patients were analyzed for cross-sectional comparison to the experimental gingivitis cohort. All of the healthy volunteers developed gingivitis after two weeks. Pyrosequencing yielded a final total of 344,267 sequences after filtering, with a mean length of 354 bases, that were clustered into an average of 299 species-level Operational Taxonomic Units (OTUs) per sample. Principal coordinates analysis (PCoA) plots revealed significant shifts in the bacterial community structure of plaque as gingivitis was induced, and community diversity increased significantly after two weeks. Changes in the relative abundance of OTUs during the transition from health to gingivitis were correlated to bleeding on probing (BoP) scores and resulted in the identification of new health- and gingivitis-associated taxa. Comparison of the healthy volunteers to the periodontitis patients also confirmed the association of a number of putative periodontal pathogens with chronic periodontitis. Taxa associated with gingivitis included Fusobacterium nucleatum subsp. polymorphum, Lachnospiraceae [G-2] sp. HOT100, Lautropia sp. HOTA94, and Prevotella oulorum, whilst Rothia dentocariosa was associated with periodontal health. Further study of these taxa is warranted and may lead to new therapeutic approaches

  4. Gingivitis

    Science.gov (United States)

    ... with some systemic diseases such as respiratory disease, diabetes, coronary artery disease, stroke and rheumatoid arthritis. Some research suggests that the bacteria responsible for periodontitis can enter your bloodstream through gum tissue, possibly ...

  5. Idiopathic gingival enlargement: A case report

    Directory of Open Access Journals (Sweden)

    Rajesh Shah

    2015-09-01

    Full Text Available Idiopathic gingival fibromatosis is a relatively rare condition characterized by the proliferation of the gingival tissues resulting in masticatory, esthetics, phonetics and psychological disturbances. We present a case with generalized diffuse gingival enlargement involving the maxillary and mandibular arches extending on buccal and lingual/palatal surfaces and covering incisal/occlusal third of the tooth in the left maxillary region. Gingivectomy was carried out in all four quadrants. Periodic recalls showed maintenance of good oral hygiene and one year follow-up revealed no recurrence.JCMS Nepal. 2015;11(1: 26-28

  6. Oral gingival metastasis: A diagnostic dilemma

    Directory of Open Access Journals (Sweden)

    Nalini Aswath

    2017-01-01

    Full Text Available Oral cavity is a rare target for metastasis with an incidence of 1% among all oral cancers. In 24% of such cases, oral metastasis is the first indication of an undiagnosed primary. Metastatic oral malignancies have been reported in the mandible, tongue, and gingiva. Although gingival metastasis has been reported from lung, prostate, rectal carcinoma in men and carcinoma of breast, adrenal glands, and genitalia in females, gingival metastasis from carcinoma of the penis has not been reported. Herein, a case of metastatic gingival carcinoma that developed after extraction of teeth from primary carcinoma of the penis is presented. An extensive literature search revealed no such similar case reports.

  7. Plasma cell gingivitis: treatment with chlorpheniramine maleate.

    Science.gov (United States)

    Ranganathan, Aravindhan Thiruputkuzhi; Chandran, Chitraa R; Prabhakar, Priya; Lakshmiganthan, Mahalingam; Parthasaradhi, Thakkalapati

    2015-01-01

    Plasma cell gingivitis is a benign lesion of unknown etiology characterized by massive and diffuse infiltration of plasma cells into the gingival connective tissue. Clinically, it can be seen as a diffuse, erythematous, and edematous swelling involving the marginal gingiva and extending into the attached gingiva. Although usually painless, the lesion can be esthetically unappealing, especially when anterior gingiva is involved. Although the usual line of management is removal of the offending agent, this report describes the treatment of plasma cell gingivitis with the topical application of chlorpheniramine maleate (25 mg) for a period of 10 days.

  8. Treatment of gingival recession in two surgical stages: Free gingival graft and connective tissue grafting.

    Science.gov (United States)

    Henriques, Paulo Sergio Gomes; Nunes, Marcelo Pereira; Pelegrine, Andre Antonio

    2011-01-01

    This report describes a clinical case of severe Miller Class II gingival recession treated by two stages of surgery that combined a free gingival graft and connective tissue grafting. First, a free gingival graft (FGG) was performed to obtain an adequate keratinized tissue level. Three months later, a connective tissue graft (CTG) was performed to obtain root coverage. The results indicated that the FGG allows for a gain in the keratinized tissue level and the CTG allows for root coverage with decreased recession level after 16 months. Therefore, for this type of specific gingival recession, the combination of FGG and CTG can be used.

  9. Low doses of X-rays induce prolonged and ATM-independent persistence of γH2AX foci in human gingival mesenchymal stem cells.

    Science.gov (United States)

    Osipov, Andreyan N; Pustovalova, Margarita; Grekhova, Anna; Eremin, Petr; Vorobyova, Natalia; Pulin, Andrey; Zhavoronkov, Alex; Roumiantsev, Sergey; Klokov, Dmitry Y; Eremin, Ilya

    2015-09-29

    Diagnostic imaging delivering low doses of radiation often accompany human mesenchymal stem cells (MSCs)-based therapies. However, effects of low dose radiation on MSCs are poorly characterized. Here we examine patterns of phosphorylated histone H2AX (γH2AX) and phospho-S1981 ATM (pATM) foci formation in human gingiva-derived MSCs exposed to X-rays in time-course and dose-response experiments. Both γH2AX and pATM foci accumulated linearly with dose early after irradiation (5-60 min), with a maximum induction observed at 30-60 min (37 ± 3 and 32 ± 3 foci/cell/Gy for γH2AX and pATM, respectively). The number of γH2AX foci produced by intermediate doses (160 and 250 mGy) significantly decreased (40-60%) between 60 and 240 min post-irradiation, indicating rejoining of DNA double-strand breaks. In contrast, γH2AX foci produced by low doses (20-80 mGy) did not change after 60 min. The number of pATM foci between 60 and 240 min decreased down to control values in a dose-independent manner. Similar kinetics was observed for pATM foci co-localized with γH2AX foci. Collectively, our results suggest differential DNA double-strand break signaling and processing in response to low vs. intermediate doses of X-rays in human MSCs. Furthermore, mechanisms governing the prolonged persistence of γH2AX foci in these cells appear to be ATM-independent.

  10. Massive pregnancy gingival enlargement: A rare case.

    Science.gov (United States)

    Srivastava, Amitabh; Gupta, Krishna Kumar; Srivastava, Sunita; Garg, Jaishree

    2013-07-01

    Gingival enlargement related to pregnancy is sometimes seen in the oral cavity. Pregnancy is a physiological state that brings full of changes in a woman's life. The metabolism and immunology of the body are modified by progesterone and estrogen as well as other local factors, these sex hormones may modify the oral mucosa and may lead to various periodontal diseases. A case of female patient 23 yrs of age reported during 8(th) month of pregnancy with a localised gingival enlargement affecting the buccal aspect of left maxillary central incisor upto canine. The hormonal changes occurring during pregnancy may be associated with generalized or localised gingival enlargement and the presence of local factors may accentuate the gingival response. Rarely the enlargement becomes maasive and protrude out extraorally.

  11. Hereditary gingival fibromatosis with distinctive facies.

    Science.gov (United States)

    Prasad, Sunkara Shree Ramalinga; Radharani, Chitturi; Sinha, Soumya; Kumar, Sv Kiran

    2012-11-01

    Hereditary gingival enlargement also known as gingivitis or familial elephantiasis is a rare type of gingival enlargement. It appears as an isolated autosomal dominant disorder or maybe associated with other conditions. Oral manifestations may vary from minimal involvement of only tuberosity area and the buccal gingiva around the lower molars to a generalized enlargement inhibiting eruption of the teeth. This paper discusses the case of a 13-year-old female patient with distinctive facial characteristics who presented to the department with a chief complaint of swollen gums since 1 year. She had severe diffuse gingival enlargement of the maxilla and mandible. Diagnosis was made based upon clinical examination and family history. Quadrant wise internal bevel gingivectomy procedure was done for the patient to restore her functional and esthetic needs.

  12. Bacterial community development in experimental gingivitis

    National Research Council Canada - National Science Library

    Kistler, James O; Booth, Veronica; Bradshaw, David J; Wade, William G

    2013-01-01

    Current knowledge of the microbial composition of dental plaque in early gingivitis is based largely on microscopy and cultural methods, which do not provide a comprehensive description of oral microbial communities...

  13. Salivary cytokine levels in early gingival inflammation

    DEFF Research Database (Denmark)

    Belstrøm, Daniel; Damgaard, Christian; Könönen, Eija

    2017-01-01

    chemoattractant protein (MCP)-1, IL-1β, and IL-1 receptor antagonist (IL-1Ra) in gingival inflammation. Twenty-eight systemically and orally healthy nonsmokers abstained from oral hygiene protocols for 10 days. After that, self-performed cleaning was resumed for 14 days. Plaque and gingival indexes were measured......Salivary protein levels have been studied in periodontitis. However, there is lack of information on salivary cytokine levels in early gingival inflammation. The aim of this study was to determine salivary levels of vascular endothelial growth factor (VEGF), interleukin (IL)-8, monocyte......, and saliva samples were collected at days 1, 4, 7, 10, and 24. Salivary cytokines were detected with Luminex®-xMAP™. Salivary IL-1β, IL-1Ra, and VEGF levels decreased after 10 days' development of experimental gingivitis and reached baseline levels at the end of the 2-week resolution period. Salivary IL-8...

  14. An unusual clinical presentation of gingival melanoacanthoma

    Directory of Open Access Journals (Sweden)

    S. P. K. Kennedy Babu

    2013-01-01

    Full Text Available Gingival melanoacanthoma is a rare, benign pigmented lesion characterized clinically by sudden onset and rapid growth of a macular brown black lesion and histologically by acanthosis of superficial epithelium and proliferation of dendritic melanocytes. This article reports a previously undescribed case of pigmented unilateral diffuse gingival enlargement, which on histopathological examination proved to be melanoacanthoma. Intraoral examination revealed pigmented unilateral diffuse gingival enlargement in relation to second and third quadrants buccally, palatally/lingually. Based on these clinical findings, gingivectomy was performed and the excised tissue was sent for biopsy. Microscopic examination revealed acanthotic and parakeratotic surface epithelium with dendritic melanocytes distributed in basal and suprabasal layers of the epithelium. 1 year follow-up recall revealed no recurrence of lesion at the surgical sites. Our patient exhibits an unusual clinical presentation of melanoacanthoma of gingiva. Pigmented gingival overgrowth of recent origin and without any etiologic factors warrants histopathologic examination.

  15. Restoring gingival harmony around single tooth implants.

    Science.gov (United States)

    Reikie, D F

    1995-07-01

    One of the biggest challenges to restoring a single tooth implant in the esthetic zone of the mouth is the creation of harmonious gingival contour around the restoration. Soft- or hard-tissue deficiencies of the edentulous space are the most common obstacles to achieving gingival symmetry around the proposed restoration. Numerous gingival and osseous grafting and regeneration techniques are available but may complicate treatment by increasing the number of surgical procedures and sites necessary. This article describes a technique for treating mild-to-moderate ridge defects without the required additional surgical procedures or a surgical donor site. Soft-tissue overcontouring is provided around the healing abutment by modification of the surgical flap at second-stage implant surgery. Subsequent gingivoplasty allows establishment of anatomic gingival architecture that surrounds the single implant prosthesis.

  16. Plasma Cell Gingivitis: An Occasional Case Report.

    Science.gov (United States)

    Mishra, M B; Sharma, Swati; Sharma, Alok

    2015-01-01

    Plasma cell gingivitis, an infrequently observed oral condition, has been clinically characterized by diffuse gingival enlargement, erythema and sometimes desquamation. These lesions are usually asymptomatic, but invariably the patient will complain of a burning sensation in the gingiva and bleeding from the mouth. The diagnosis requires hematological screening in addition to clinical and histopathological examinations. This case report outlines one such case of plasma cell gingivitis in a 15-year-old female caused by use of an herbal, homemade toothpowder. The case presented here highlights the adverse effects and irrational use of herbal agents in dentifrices. At the same time, it emphasizes the need for comprehensive history taking, careful clinical examination and appropriate diagnostic tests in order to arrive at a definitive diagnosis and treatment plan for gingival conditions that are refractory to conventional therapy and to exclude certain malignancies and oral manifestations of systemic diseases.

  17. Phenytoin- and amlodipine-induced gingival overgrowth

    Directory of Open Access Journals (Sweden)

    Ching-Wen Chang

    2012-03-01

    Full Text Available Drug-induced gingival overgrowth is an adverse event associated with three types of drugs, i.e., anticonvulsants, immunosuppressants, and calcium-channel blockers. It was shown that the combined use of an immunosuppressant (cyclosporine and a calcium-channel blocker increases the prevalence and severity of gingival overgrowth. However, few reports discussed the effects of the combination of an anticonvulsant (phenytoin and a calcium-channel blocker (amlodipine. In this case report, we present an epilepsy patient who was using both phenytoin and amlodipine, which caused extensive gingival overgrowth. After periodontal treatment and a gingivectomy, the gingival overgrowth was significantly reduced. A postoperative drug-substitution regimen and intensive professional care ensured a stable result 1 year after surgery.

  18. Massive pregnancy gingival enlargement: A rare case

    OpenAIRE

    2013-01-01

    Gingival enlargement related to pregnancy is sometimes seen in the oral cavity. Pregnancy is a physiological state that brings full of changes in a woman's life. The metabolism and immunology of the body are modified by progesterone and estrogen as well as other local factors, these sex hormones may modify the oral mucosa and may lead to various periodontal diseases. A case of female patient 23 yrs of age reported during 8th month of pregnancy with a localised gingival enlargement affecting t...

  19. NIFEDIPINE - INDUCED GINGIVAL ENLARGEMENT (IN SPANISH)

    OpenAIRE

    Manzur-Villalobos Isabella; Manzur-Jattin Fernando; Díaz-Caballero Antonio José

    2014-01-01

    Introduction: the gingival enlargement is the increase of the size of the gingiva caused by diverse factors, between which are the drugs, mainly the antihypertensive, immunosuppressive and anticonvulsant medications. Between the first ones, the nifedipine, an antagonist of the calcium, is one of the most frequently indicated. Clinical case: A 62-year-old-male patient with arterial hypertension treated with nifedipine for more of fifteenth years, who presented chronic gingival e...

  20. Association among pain, masticatory performance, and proinflammatory cytokines in crevicular fluid during orthodontic treatment.

    Science.gov (United States)

    Gameiro, Gustavo Hauber; Schultz, Christian; Trein, Marcos Porto; Mundstock, Karina Santos; Weidlich, Patrícia; Goularte, Jéferson Ferraz

    2015-12-01

    Orthodontic patients usually complain about masticatory limitations associated with the activation of fixed appliances. The aim of this investigation was to evaluate whether orthodontic pain reflects differences in the objective evaluation of mastication and in the levels of proinflammatory cytokines in the crevicular fluid of patients undergoing orthodontic treatment. Twenty patients with malocclusions requiring orthodontic treatment were included in this prospective study. Their pain experience, masticatory performance, and levels of interleukin 1-beta and prostaglandin E2 in crevicular fluid were evaluated at 3 times: before bracket placement, 24 hours after archwire placement, and 30 days after the initial appointment. All variables were compared with those of a control group of 25 subjects with normal occlusion. The masticatory performance of the patients was significantly reduced at 24 hours after bracket placement, the period in which they reported higher values of pain and had higher levels of interleukin 1-beta. The levels of prostaglandin E2 did not change in the periods evaluated, and there were no correlations between the levels of cytokines and the functional limitations observed. The only significant correlation was between pain and decreased masticatory performance. The masticatory performance of orthodontic patients is significantly reduced only during the period of greatest pain. However, these alterations did not correlate with any measurement of interleukin 1-beta or prostaglandin E2 in the crevicular fluid, suggesting that these solitary measurements are inadequate to predict the temporary pain and masticatory limitations experienced by patients undergoing orthodontic treatment. Copyright © 2015 American Association of Orthodontists. Published by Elsevier Inc. All rights reserved.

  1. Effect of platelet rich fibrin on proliferation and differentiation of human gingival fibroblasts on rough titanium surface%富血小板纤维蛋白对粗糙钛板表面牙龈成纤维细胞增殖和分化的影响

    Institute of Scientific and Technical Information of China (English)

    2013-01-01

    Objective To study the effect of platelet rich fibrin (PRF) on proliferation and differentiation of human gingival fibroblasts on rough titanium surface. Methods Human gingival fibroblasts were cultured in vitro, and their fourth generation was co-cultured with rough titanium. The cells were then cultured in common medium and PRF medium respectively, and detected by MTT, RT-PCR and Western blot respectively. Results MTT showed that PRF could promote the proliferation of human gingival fibroblasts(P < 0.05). RT-PCR demonstrated that the expression level of typeⅠcollagen mRNA was higher than that of bFGF mRNA in PRF medium than in common medium(P<0.05). Western blot displayed that the expression level of typeⅠcollagen was higher in PRF medium than in common medium(P<0.05). Conclusion PRF can accelerate the proliferation and differentiation of human gingival fibroblasts on rough titanium surface.%  目的探讨富血小板纤维蛋白对粗糙钛板表面人牙龈成纤维细胞增殖和分化的影响。方法体外培养人牙龈成纤维细胞,传至第4代与粗糙钛板联合培养用于实验。分别在普通培养基和含富血小板纤维蛋白的培养基中培养,MTT、RT-PCR、蛋白印迹技术进行检测。结果 MTT结果显示含加入富血小板纤维蛋白能明显促进粗糙钛板表面人牙龈成纤维细胞的增殖,差异有统计学意义(P<0.05)。RT-PCR提示两组均有Type(Ⅰ)collagen和bFGF的mRNA表达,含富血小板纤维蛋白组mRNA表达比普通组明显增强。WB提示,Ⅰ型胶原蛋白表达在含富血小板纤维蛋白组中明显增强。结论富血小板纤维蛋白能促进粗糙钛板表面人牙龈成纤维细胞的增殖和分化。

  2. A Pyrosequencing Investigation of Differences in the Feline Subgingival Microbiota in Health, Gingivitis and Mild Periodontitis.

    Science.gov (United States)

    Harris, Stephen; Croft, Julie; O'Flynn, Ciaran; Deusch, Oliver; Colyer, Alison; Allsopp, Judi; Milella, Lisa; Davis, Ian J

    2015-01-01

    Periodontitis is the most frequently diagnosed health problem in cats yet little is known about the bacterial species important for the disease. The objective of this study was to identify bacterial species associated with health, gingivitis or mild periodontitis (gingivitis or mild periodontitis. Pyrosequencing of the V1-V3 region of the 16S rDNA from these plaque samples generated more than one million reads and identified a total of 267 operational taxonomic units after bioinformatic and statistical analysis. Porphyromonas was the most abundant genus in all gingival health categories, particularly in health along with Moraxella and Fusobacteria. The Peptostreptococcaceae were the most abundant family in gingivitis and mild periodontitis. Logistic regression analysis identified species from various genera that were significantly associated with health, gingivitis or mild periodontitis. The species identified were very similar to those observed in canine plaque in the corresponding health and disease states. Such similarities were not observed between cat and human at the bacterial species level but with disease progression similarities did emerge at the phylum level. This suggests that interventions targeted at human pathogenic species will not be effective for use in cats but there is more potential for commonalities in interventions for cats and dogs.

  3. A Pyrosequencing Investigation of Differences in the Feline Subgingival Microbiota in Health, Gingivitis and Mild Periodontitis.

    Directory of Open Access Journals (Sweden)

    Stephen Harris

    Full Text Available Periodontitis is the most frequently diagnosed health problem in cats yet little is known about the bacterial species important for the disease. The objective of this study was to identify bacterial species associated with health, gingivitis or mild periodontitis (<25% attachment loss in feline plaque. Knowledge of these species is a first step in understanding the potential for improving oral health of cats via dietary interventions that alter the proportions of influential species. Subgingival plaque samples were collected from 92 cats with healthy gingiva, gingivitis or mild periodontitis. Pyrosequencing of the V1-V3 region of the 16S rDNA from these plaque samples generated more than one million reads and identified a total of 267 operational taxonomic units after bioinformatic and statistical analysis. Porphyromonas was the most abundant genus in all gingival health categories, particularly in health along with Moraxella and Fusobacteria. The Peptostreptococcaceae were the most abundant family in gingivitis and mild periodontitis. Logistic regression analysis identified species from various genera that were significantly associated with health, gingivitis or mild periodontitis. The species identified were very similar to those observed in canine plaque in the corresponding health and disease states. Such similarities were not observed between cat and human at the bacterial species level but with disease progression similarities did emerge at the phylum level. This suggests that interventions targeted at human pathogenic species will not be effective for use in cats but there is more potential for commonalities in interventions for cats and dogs.

  4. Association of a genetic polymorphism (-44 C/G SNP in the human DEFB1 gene with expression and inducibility of multiple β-defensins in gingival keratinocytes

    Directory of Open Access Journals (Sweden)

    Dommisch Henrik

    2009-08-01

    Full Text Available Abstract Background Human β-defensins (hBDs are antimicrobial peptides with a role in innate immune defense. Our laboratory previously showed that a single nucleotide polymorphism (SNP in the 5' untranslated region of the hBD1 gene (DEFB1, denoted -44 (rs1800972, is correlated with protection from oral Candida. Because this SNP alters the putative mRNA structure, we hypothesized that it alters hBD1 expression. Methods Transfection of reporter constructs and evaluation of antimicrobial activity and mRNA expression levels in keratinocytes from multiple donors were used to evaluate the effect of this SNP on constitutive and induced levels of expression. Results Transfection of CAT reporter constructs containing the 5' untranslated region showed that the -44 G allele yielded a 2-fold increase in CAT protein compared to other common haplotypes suggesting a cis effect on transcription or translation. The constitutive hBD1 mRNA level in human oral keratinocytes was significantly greater in cells from donors with the -44 GG genotype compared to those with the common CC genotype. Surprisingly, the hBD3 mRNA level as well as antimicrobial activity of keratinocyte extracts also correlated with the -44 G allele. Induced levels of hBD1, hBD2, and hBD3 mRNA were evaluated in keratinocytes challenged with Toll-like receptor 2 and 4 ligands, interleukin-1β, TNFα, and interferon-γ (IFNγ. In contrast to constitutive expression levels, IFNγ-induced keratinocyte hBD1 and hBD3 mRNA expression was significantly greater in cells with the common CC genotype, but there was no clear correlation of genotype with hBD2 expression. Conclusion The DEFB1 -44 G allele is associated with an increase in overall constitutive antimicrobial activity and expression of hBD1 and hBD3 in a manner that is consistent with protection from candidiasis, while the more common C allele is associated with IFNγ inducibility of these β-defensins and is likely to be more protective in

  5. ANGKA KEJADIAN KARIES DAN GINGIVITIS PADA ANAK SEKOLAH DASAR USIA 8-12 TAHUN DI KABUPATEN MAROS TAHUN 2014

    OpenAIRE

    HANAPI, ADE NURZAQIAH

    2014-01-01

    2014 Teeth are part of the tools of mastication on the digestive system in the human body. The main problem child oral health are dental caries. Dental caries in children is a serious problem in oral health in Indonesia, with a prevalence of up to 90.05% .Other diseases that often accompany dental caries is a disease of periodontal tissues. Gingivitis is an inflammatory process in the periodontal tissue is confined to the gingiva and gingivitis reversibel.a...

  6. LACK OF ASSOCIATION BETWEEN HERPESVIRUS DETECTION IN SALIVA AND GINGIVITIS IN HIV‑INFECTED CHILDREN

    Directory of Open Access Journals (Sweden)

    Renata A. OTERO

    2015-06-01

    Full Text Available The aims of this study were to compare the detection of human herpesviruses (HHVs in the saliva of HIV-infected and healthy control children, and to evaluate associations between viral infection and gingivitis and immunodeficiency. Saliva samples were collected from 48 HIV-infected and 48 healthy control children. Clinical and laboratory data were collected during dental visits and from medical records. A trained dentist determined gingival indices and extension of gingivitis. Saliva samples were tested for herpes simplex virus types 1 and 2 (HSV-1 and HSV-2, varicella zoster virus (VZV, Epstein-Barr virus (EBV, and cytomegalovirus (CMV by nested polymerase chain reaction assays. Thirty-five HIV-infected and 16 control children had gingivitis. Seventeen (35.4% HIV-infected children and 13 (27% control children were positive for HHVs. CMV was the most commonly detected HHV in both groups (HIV-infected, 25%; control, 12.5%, followed by HSV-1 (6.2% in both groups and HSV-2 (HIV-infected, 4.2%; control, 8.3%. The presence of HHVs in saliva was not associated with the presence of gingivitis in HIV-1-infected children (p = 0.104 or healthy control children (p = 0.251, or with immunosuppression in HIV-infected individuals (p = 0.447. Gingivitis was correlated with HIV infection (p = 0.0001. These results suggest that asymptomatic salivary detection of HHVs is common in HIV-infected and healthy children, and that it is not associated with gingivitis.

  7. LACK OF ASSOCIATION BETWEEN HERPESVIRUS DETECTION IN SALIVA AND GINGIVITIS IN HIV‑INFECTED CHILDREN.

    Science.gov (United States)

    Otero, Renata A; Nascimento, Flávia N N; Souza, Ivete P R; Silva, Raquel C; Lima, Rodrigo S; Robaina, Tatiana F; Câmara, Fernando P; Santos, Norma; Castro, Gloria F

    2015-01-01

    The aims of this study were to compare the detection of human herpesviruses (HHVs) in the saliva of HIV-infected and healthy control children, and to evaluate associations between viral infection and gingivitis and immunodeficiency. Saliva samples were collected from 48 HIV-infected and 48 healthy control children. Clinical and laboratory data were collected during dental visits and from medical records. A trained dentist determined gingival indices and extension of gingivitis. Saliva samples were tested for herpes simplex virus types 1 and 2 (HSV-1 and HSV-2), varicella zoster virus (VZV), Epstein-Barr virus (EBV), and cytomegalovirus (CMV) by nested polymerase chain reaction assays. Thirty-five HIV-infected and 16 control children had gingivitis. Seventeen (35.4%) HIV-infected children and 13 (27%) control children were positive for HHVs. CMV was the most commonly detected HHV in both groups (HIV-infected, 25%; control, 12.5%), followed by HSV-1 (6.2% in both groups) and HSV-2 (HIV-infected, 4.2%; control, 8.3%). The presence of HHVs in saliva was not associated with the presence of gingivitis in HIV-1-infected children (p = 0.104) or healthy control children (p = 0.251), or with immunosuppression in HIV-infected individuals (p = 0.447). Gingivitis was correlated with HIV infection (p = 0.0001). These results suggest that asymptomatic salivary detection of HHVs is common in HIV-infected and healthy children, and that it is not associated with gingivitis.

  8. Effects of immunization with natural and recombinant lysine decarboxylase on canine gingivitis development.

    Science.gov (United States)

    Peters, Jennifer L; DeMars, Paul L; Collins, Lindsay M; Stoner, Julie A; Matsumoto, Hiroyuki; Komori, Naoka; Singh, Anil; Feasley, Christa L; Haddock, James A; Levine, Martin

    2012-10-19

    Periodontal disease, gingival inflammation (gingivitis) and periodontal attachment loss (periodontitis), causes tooth loss and susceptibility to chronic inflammation. Professionally scaling and cleaning the teeth regularly controls the disease, but is expensive in companion animals. Eikenella corrodens is common in canine oral cavities where it is a source of lysine decarboxylase (LDC). In human dental biofilms (plaques), LDC converts lysine to cadaverine and impairs the gingival epithelial barrier to bacteria. LDC vaccination may therefore retard gingivitis development. Year-old beagle dogs provided blood samples, and had weight and clinical measurements (biofilm and gingivitis) recorded. After scaling and cleaning, two dogs were immunized subcutaneously with 0.2mg native LDC from E. corrodens and 2 sets of four dogs with 0.2mg recombinant LDC purified from Escherichia coli. A third set of 4 dogs was immunized intranasally. Rehydragel(®), Emulsigen(®), Polygen™ or Carbigen™ were used as adjuvant. Four additional pairs of dogs were sham-immunized with each adjuvant alone (controls). Immunizations were repeated twice, 3 weeks apart, and clinical measurements were obtained after another 2 weeks, when the teeth were scaled and cleaned again. Tooth brushing was then stopped and the diet was changed from hard to soft chow. Clinical measurements were repeated after 1, 2, 3, 4, 6 and 8 weeks. Compared with sham-immunized dogs, gingivitis was reduced over all 8 weeks of soft diet after subcutaneous immunization with native LDC, or after intranasal immunization with recombinant LDC in Carbigen™, but for only 6 of the 8 weeks after subcutaneous immunization with recombinant LDC in Emulsigen(®) (repeated measures ANOVA). Subcutaneous vaccination induced a strong serum IgG antibody response that decreased during the soft diet period, whereas intranasal immunization induced a weak serum IgA antibody response that did not decrease. Immunization with recombinant LDC may

  9. An unusual case of generalized severe gingival enlargement during pregnancy.

    Science.gov (United States)

    McIntosh, Crystal L; Kolhatkar, Shilpa; Winkler, James R; Ojha, Junu; Bhola, Monish

    2010-01-01

    Increased hormone levels that are present during puberty and pregnancy are associated with localized or generalized gingival enlargement. This article reviews the gingival alterations that can occur during pregnancy and describes a case of generalized severe gingival enlargement associated with pregnancy and its management. A 36-year-old woman had severe bilateral gingival enlargement of short duration. The patient denied taking any medications. The laboratory report revealed no systemic abnormalities; however, the report disclosed that she was pregnant. Surgical therapy for the gingival enlargement included gingivectomy and gingivoplasty of all quadrants, which reduced the size of the enlarged gingiva. Postoperative visits demonstrated uneventful healing, with no recurrence seen at the one-year follow-up appointment. It appears that the English literature includes only one other case report that discusses generalized gingival enlargement during pregnancy. Pregnancy-related gingival enlargement should be included as a differential diagnosis in women who have non-drug-induced generalized gingival enlargement.

  10. Diagnostic considerations concerning a case of an unusual gingivitis

    NARCIS (Netherlands)

    van der Haring, I.S.; Witjes, M.J.H.

    2006-01-01

    A young woman presented a severe gingivitis that wouldn't respond to antibiotics prescribed by her general practitioner. Thorough clinical examination showed atypical gingival inflammation. In such unusual cases a careful anamnesis is essential in determining appropriate continued diagnostic

  11. A laser Doppler study of gingival blood flow variations following periosteal stimulation.

    Science.gov (United States)

    Ambrosini, Pascal; Cherene, Sabine; Miller, Neal; Weissenbach, Michel; Penaud, Jacques

    2002-02-01

    Evaluation of the modifications occurring in human gingival blood flow following periosteal stimulation. Laser Doppler was used to measure the gingival blood flow (GBF). The reproducibility of the technique was validated by comparing measures made at intervals of 1 week. Sensitivity was verified by recording GBF before and after injection of an anesthetic containing a vasoconstrictor. Finally, 12 patients were subjected to GBF measurements before and 8 days after periosteal stimulation prior to gingival grafting. The laser Doppler accurately measured GBF. Measurements made at day 0 and day 7 were not statistically different (p=0.60). After injection of an anesthesic solution containing vasoconstrictor, the laser Doppler recorded a sharp decrease of the GBF (p=0.04). The patient that underwent periosteal stimulation showed statistically significant increases (p=0.02) in GBF before and 1 week post-stimulation. Periosteal stimulation induces significant increases in the GBF after 1 week.

  12. Gingival and periodontal ligament fibroblasts differ in their inflammatory response to viable Porphyromonas gingivalis

    NARCIS (Netherlands)

    Scheres, N.; Laine, M.L.; de Vries, T.J.; Everts, V.; van Winkelhoff, A.J.

    2010-01-01

    Background and Objective: Porphyromonas gingivalis is an oral pathogen strongly associated with destruction of the tooth-supporting tissues in human periodontitis. Gingival fibroblasts (GF) and periodontal ligament fibroblasts (PDLF) are functionally different cell types in the periodontium that can

  13. Effects of alpha-tocopherol on gingival expression of inducible nitric ...

    African Journals Online (AJOL)

    2015-09-01

    Sep 1, 2015 ... reported to enhance cellular proliferation and wound healing, suppress iNOS production, and provide protection against oxidative damage. .... cytokines from human gingival fibroblasts[29] as TNF‑α and interleukin‑1β, and that .... The role of reactive oxygen and antioxidant species in periodontal tissue ...

  14. Jatropha curcas latex inhibits the release of collagenase by gingival fibroblast

    Directory of Open Access Journals (Sweden)

    Fazwishni Siregar

    2007-12-01

    Full Text Available Folkloric use of J. curcas latex among others are to cure tooth pain, bleeding gum and as anti-inflammatory drug. Collagenase is a neutral protease released by activated macrophage and also by fibroblasts in small amounts. The aim of this study was to evaluate the effect of J. curcas latex on collagenase released by fibroblasts. Four doses of J. curcas latex from 37.5-300 g/ml were added to 3 human gingival primary fibroblast cell culture. After 1 to 4 days of incubation, collagenase in the supernatant was assayed with collagen. The degradation products were then separated by SDS-PAGE and the density of ¾ A bands were measured semi quantitatively by Adobe Photo computer program. Result showed that J. curcas latex decreased collagenase released by human gingival fibroblast, and increasing dose inhibits more. It may be concluded that the latex of J. curcas inhibits the release of collagenase by human gingival fibroblast. (Med J Indones 2007; 16:219-23Keywords: Jatropha curcas, collagenase, human gingival fibroblast, collagenase assay, SDS-PAGE

  15. Effect of a chlorhexidine mouthrinse on plaque, gingival inflammation and staining in gingivitis patients: a systematic review

    NARCIS (Netherlands)

    Van Strydonck, D.A.C.; Slot, D.E.; van der Velden, U.; van der Weijden, F.

    2012-01-01

    AIM: To systematically evaluate the efficacy of chlorhexidine (CHX) mouthrinses on plaque, gingival inflammation and staining in gingivitis patients. MATERIAL & METHODS: Medline, EMBASE and Cochrane Central Register of Controlled Trials were searched through April 2011. Randomized controlled

  16. [Neutrophils and monocytes in gingival epithelium

    Science.gov (United States)

    Meng, H X; Zheng, L P

    1994-06-01

    Neutrophils and monocytes of gingival epithellium in health gingiva(H),marginal gingivitis(MG),juvenile periodontitis(JP),adult periodontitis(AP) and subgingival bacteria were quantitated and analyzed,The results showed that the numbers of PMN within either pocket epithelium or oral gingival epithelium in JP were significantly lower than in AP and G.The amounts of PMN in AP were much larger than other three groups.Positive correlation between the number of PMN in sulcular pocket epitelium and the motile bacteri of subgingival plaque was demonstrated by correlation analysis.Monocytes mainly presented in deep pocket and junctional epithelum which were stained by NAE method,however very few Langhans cells were seen in these areas.

  17. Gingival Harmony in Anterior Aesthetic Restorations

    Directory of Open Access Journals (Sweden)

    Dalenda Hadyaoui

    2014-12-01

    Full Text Available This article describes a case of gingival asymmetry with compromising aesthetics. A 25-year-old dental student presented to the department of prosthetic dentistry. She was concerned about the greyish transparency of the crown metal margin through the marginal gingiva. The crown was placed to restore her lateral incisor. A comprehensive examination revealed that this unaesthetic aspect was caused by a non-harmonious gingival architecture in the lateral incisor marked by an unaesthetic gingival Zenith. The treatment plan included a surgical crown lengthening followed by prosthetic therapy consisting in a Zirconia based crown replacing the old prosthesis. Thanks to a well-planned multi-disciplinary approach, the result was esthetically acceptable and the patient was satisfied.

  18. Gingival recession, oral hygiene and associated factors among ...

    African Journals Online (AJOL)

    ... the presence of plaque, calculus, gingival bleeding and gingival recession at six sites ... Results: The prevalence of gingival recession (GR) > 1mm was 33.6%, calculus 99.3%, plaque 100%, and ... http://dx.doi.org/10.4314/eamj.v86i3.54967.

  19. Diverse modalities of gingival replacement: A report of three cases

    Directory of Open Access Journals (Sweden)

    Dileep N Vinnakota

    2012-01-01

    Full Text Available Gingival replacement is often a component of comprehensive prosthodontics. Gingival prostheses may be fixed or removable. It can be made from acrylics, composite resins, silicones or porcelain-based materials.This paper describes different clinical situations in which three types of gingival prostheses, removable acrylic veneer with melanin pigmentation, fixed ceramic veneer and flexible nylon based veneer, were used effectively.

  20. An unusual case of idiopathic gingival fibromatosis

    Directory of Open Access Journals (Sweden)

    Vikender S Yadav

    2013-01-01

    Full Text Available Idiopathic gingival fibromatosisis, a condition of undetermined cause can develop as an isolated disorder, but mostly it is associated with some syndrome. It usually begins at the time of eruption of permanent teeth but can develop with the eruption of deciduous dentition and rarely present at birth. This case report describes an unusual case of non-syndromic generalized idiopathic gingival fibromatosis in a 15-year-old male present since birth. Surgical treatment in the form of ledge and wedge procedure with internal bevel gingivectomy was performed. No recurrence of enlargement was seen after 2 years of follow-up.

  1. [Frenectomy associated with a triangular gingival graft].

    Science.gov (United States)

    Borghetti, A; Guy, J P; Cesano, B

    1991-11-01

    In periodontal therapy, frenectomy is indicated when the frenum exerts tension on the gingival margin and interferes with proper oral hygiene. The procedure is also employed when the frenum prevents closure of a diastema during orthodontic therapy. Frenectomy should be done after the canines have erupted and before retention is started to prevent separation of the teeth. For an improved surgical and cosmetic result, the authors propose a triangular-shaped gingival graft after the frenum has been excised. The advantage of the procedure is to create an area of attached gingiva and enhance healing.

  2. Masking the unmasked-gingival veneer

    Directory of Open Access Journals (Sweden)

    Dipti Sanghavi

    2014-01-01

    Full Text Available Black triangles are third most disliked aesthetic problem after caries and crown margins. Periodontal disease is one of most common reason for black triangles. The dental aesthetics is a fine balance between white and pink component surrounding natural teeth and their replacements. Gingival veneer is of importance in periodontal conditions where multiple teeth are affected with alveolar bone loss and surgical correction is not a feasible option. Gingival veneer is noninvasive, economical, and less time consuming treatment option large areas of aesthetic and functional deficit.

  3. Aggressive Periodontitis with Streptococcal Gingivitis a Case Report

    Directory of Open Access Journals (Sweden)

    Gaurav Solanki

    2012-10-01

    Full Text Available Acute streptococcal gingivitis is an acute inflammation of the oral mucosa and also seen with the other oral diseases as aggressive periodontitis. Streptococcal infections of gingiva are seen rarely; also the origin of this gingival inflammation is occasionally different from that of routine plaque associated gingivitis. This case report describes a patient who presented with severe gingival inflammation and attachment loss that was diagnosed as an acute streptococcal infection associated with aggressive periodontitis. A case of aggressive periodontitis with streptococcal gingivitis was reported which was diagnosed treated with no postoperative complications.

  4. Gingival overgrowth: Part 1: aetiology and clinical diagnosis.

    Science.gov (United States)

    Beaumont, J; Chesterman, J; Kellett, M; Durey, K

    2017-01-27

    Most commonly, gingival overgrowth is a plaque-induced inflammatory process, which can be modified by systemic disease or medications. However, rare genetic conditions can result in gingival overgrowth with non-plaque-induced aetiology. It is also important to appreciate the potential differential diagnoses of other presentations of enlarged gingival tissues; some may be secondary to localised trauma or non-plaque-induced inflammation and, albeit rarely, others may be manifestations of more sinister diseases or lesions. A definitive diagnosis will then enable an appropriate management strategy. This paper aims to discuss clinical features and diagnoses for conditions presenting with gingival overgrowth and other enlargements of gingival tissues.

  5. 冠向复位瓣术治疗牙龈退缩的临床研究%Coronally positioned flap procedure in the treatment of human gingival recession

    Institute of Scientific and Technical Information of China (English)

    于春梅; 陈巨峰; 方溢云; 李嘉朋; 卢钰芬

    2009-01-01

    AIM:To compare the clinical outcome following treatment of local gingival recessions by coronal ly positioned flap with a bioabsorbable membrane or by a coronally positioned flap procedure alone. METHODS: Twenty patients with buccal bilateral Miller Class Ⅰ or Class Ⅱ gingival recession defects were treated randomly by coro nally positioned flap with a bioabsorbable membrane or a coronally positioned flap alone (20 sites). The treatment sites were selected randomly. Twenty sites in the membrane group and 20 sites in the non - membrane group were examinat ed at baseline, and at 3 and 6 months postoperatively. Clinical variables included the apical extent of the gingival re cession ( RD), the width of the gingival recession ( RW) at the cemento - enamel junction ( CEJ), the probing depth ( PD ) and the clinical attachment level ( CAL). RESULTS: Both treatments resulted in a significant gain of root cov erage(p 0.05). Statistical difference could be found in the results of PD, CAL but non in the results of RD and RW between two groups. CONCLUSION: Compared with coronally positioned flap combined with a bioabsorbable membrane , coronally positioned flap procedure alone can get more root coverage in vertical and horizontal direc tions. The coronally positioned flap operation offers a predictable and simple approach as a root coverage procedure for 2.5 mm~5mm recession defects .%目的:单纯冠向复位瓣技术(Coronally positioned flap,CPF)与冠向复位瓣技术联合引导组织再生技术(guided tissue regeneration,GTR)治疗牙龈退缩临床效果的比较.方法:选择牙弓两侧均有唇或颊侧牙龈退缩的病人20例(Miller分度Ⅰ或Ⅱ度,2.5 mm0.05);两组间的CAL、PD结果无统计学差异(P>0.05);RD、RW有统计学差异(P<0.05);结论:冠向复位瓣技术治疗2.5-5 mm的牙龈退缩可在垂直和水平方向获得更多的根面覆盖,与冠向复位瓣技术联合引导组织再生技术相比具有一定的临床优越性.

  6. Sangramiento gingival y flora bacteriana en la gingivitis y la periodontitis

    Directory of Open Access Journals (Sweden)

    Iriam Baldemira Rodríguez

    1996-08-01

    Full Text Available Se estudiaron 30 sitios o áreas periodontales que presentaban gingivitis y 30 con periodontitis, con el objetivo de determinar la relación existente entre el sangramiento gingival y la flora microbiana presente en la gingivitis y la periodontitis. Los pacientes seleccionados no presentaban antecedentes de enfermedad general y no habían recibido medicación antimicrobiana ni tratamiento periodontal en los útimos 6 meses; en el caso de las mujeres, no podían estar embarazadas. En los dientes seleccionados se procedió a tomar la muestra cumpliendo con los requisitos establecidos; luego se examinó inmediatamente en el microscopio de campo oscuro. Los resultados obtenidos indican que no hubo relación entre los morfotipos microbianos y los diferentes valores del índice de sangramiento gingival.Thirty periodontal sites presenting with gingivitis and 30 with periodontitis were studied with the aim of determining the relation between gingival bleeding and microflora present in gingivitis and periodontitis. Patients selected for the study did not present with a history of systemic diseases and received neither antimicrobial medication nor periodontal treatment during the last 6 months, in the case of women it was required that they were not pregnant. The sample was taken in the teeth chosen in compliance with the requirements established; then the sample was immediately examined in the dark field microscope. Results obtained suggest that there was no relationship between microbial morphological types and the different values of the gingival bleeding index.

  7. The relation of gingival thickness to dynamics of gingival margin position pre- and post-surgically

    Directory of Open Access Journals (Sweden)

    Kharidhi Laxman Vandana

    2016-01-01

    Full Text Available Background: To evaluate the gingival margin position (GMP before and after open flap debridement in different gingival thickness (GT. Materials and Methods: Twenty-seven healthy patients with moderate to advanced adult periodontitis were included in a randomized control clinical trial. A calibrated UNC-15 periodontal probe, an occlusal onlay stent was used for clinical measurements recorded at baseline, 3 month, 6 month, and 16 month. The changes in the GMP were studied at midbuccal (Mi-B, mesiobuccal (MB, and distobuccal sites. GT was measured presurgically, transgingivally at Mi-B and interdental sites, divided into 2 groups: Group 1 (thin and Group 2 (thick. Results: In GT of ≤1 mm group, the statistically significant apical shift of GMP led to gingival recession at all study sites in the early postsurgical period of 1 and 3 months. During 6 and 16 months, the apical shift of GMP coincided with the Chernihiv Airport at Mi-B site (6 months, MB site (16 months. The gingival recession was obvious at Mi-B sites (16 months. In the GT of >1 mm, the statistically significant apical shift of GMP did not cause gingival recession at any sites throughout postsurgical (1, 3, 6, and 16 months period. Conclusion: Thin gingiva showed apical shift of GMP leading to gingival recession as compared to thick gingiva postsurgically.

  8. Prevention of gingivitis: Oral hygiene and dentifrices

    NARCIS (Netherlands)

    Sälzer, S.A.

    2016-01-01

    At the basis of Oral Health lies daily oral hygiene self-care with the result, if correctly performed, of plaque and gingivitis reduction. Epidemiological studies indicate that the level of oral hygiene in the general population has increased over the last decades. However, there still appears to be

  9. Prevention of gingivitis: Oral hygiene and dentifrices

    NARCIS (Netherlands)

    Sälzer, S.A.

    2016-01-01

    At the basis of Oral Health lies daily oral hygiene self-care with the result, if correctly performed, of plaque and gingivitis reduction. Epidemiological studies indicate that the level of oral hygiene in the general population has increased over the last decades. However, there still appears to be

  10. A Pyrosequencing Investigation of Differences in the Feline Subgingival Microbiota in Health, Gingivitis and Mild Periodontitis

    Science.gov (United States)

    Harris, Stephen; Croft, Julie; O’Flynn, Ciaran; Deusch, Oliver; Colyer, Alison; Allsopp, Judi; Milella, Lisa; Davis, Ian J.

    2015-01-01

    Periodontitis is the most frequently diagnosed health problem in cats yet little is known about the bacterial species important for the disease. The objective of this study was to identify bacterial species associated with health, gingivitis or mild periodontitis (periodontitis. Pyrosequencing of the V1-V3 region of the 16S rDNA from these plaque samples generated more than one million reads and identified a total of 267 operational taxonomic units after bioinformatic and statistical analysis. Porphyromonas was the most abundant genus in all gingival health categories, particularly in health along with Moraxella and Fusobacteria. The Peptostreptococcaceae were the most abundant family in gingivitis and mild periodontitis. Logistic regression analysis identified species from various genera that were significantly associated with health, gingivitis or mild periodontitis. The species identified were very similar to those observed in canine plaque in the corresponding health and disease states. Such similarities were not observed between cat and human at the bacterial species level but with disease progression similarities did emerge at the phylum level. This suggests that interventions targeted at human pathogenic species will not be effective for use in cats but there is more potential for commonalities in interventions for cats and dogs. PMID:26605793