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Sample records for human engineering bibliography

  1. Aeronautical engineering. A continuing bibliography with indexes

    Science.gov (United States)

    1982-01-01

    This bibliography lists 326 reports, articles, and other documents introduced into the NASA scientific and technical information system in January 1982. Topics on aeronautical engineering and aerodynamics such as flight control systems, avionics, computer programs, computational fluid dynamics and composite structures are covered.

  2. Annotated bibliography of software engineering laboratory literature

    Science.gov (United States)

    Kistler, David; Bristow, John; Smith, Don

    1994-01-01

    This document is an annotated bibliography of technical papers, documents, and memorandums produced by or related to the Software Engineering Laboratory. Nearly 200 publications are summarized. These publications cover many areas of software engineering and range from research reports to software documentation. This document has been updated and reorganized substantially since the original version (SEL-82-006, November 1982). All materials have been grouped into eight general subject areas for easy reference: (1) The Software Engineering Laboratory; (2) The Software Engineering Laboratory: Software Development Documents; (3) Software Tools; (4) Software Models; (5) Software Measurement; (6) Technology Evaluations; (7) Ada Technology; and (8) Data Collection. This document contains an index of these publications classified by individual author.

  3. A Selected, Annotated Bibliography on Employment of Minority Engineers.

    Science.gov (United States)

    National Academy of Sciences - National Research Council, Washington, DC. Assembly of Engineering.

    The annotated bibliography is intended to inform those concerned with personnel guidance, recruiting, and hiring in industry, research, education, and government about the available publications relating to the employment of Black, Mexican American, Puerto Rican, and American Indian engineers. To facilitate its usefulness, the bibliography is…

  4. Annotated research bibliography for geothermal reservoir engineering

    Energy Technology Data Exchange (ETDEWEB)

    Sudol, G.A.; Harrison, R.F.; Ramey, H.J. Jr.

    1979-08-01

    This bibliography is divided into the following subject areas: formation evaluation, modeling, exploitation strategies, and interpretation of production trends. A subject/author index is included. (MHR)

  5. Annotated bibliography of human factors applications literature

    Energy Technology Data Exchange (ETDEWEB)

    McCafferty, D.B.

    1984-09-30

    This bibliography was prepared as part of the Human Factors Technology Project, FY 1984, sponsored by the Office of Nuclear Safety, US Department of Energy. The project was conducted by Lawrence Livermore National Laboratory, with Essex Corporation as a subcontractor. The material presented here is a revision and expansion of the bibliographic material developed in FY 1982 as part of a previous Human Factors Technology Project. The previous bibliography was published September 30, 1982, as Attachment 1 to the FY 1982 Project Status Report.

  6. A Selective Bibliography of Commercial Radio and Television Engineering.

    Science.gov (United States)

    Losee, Robert M., Jr.

    A selective bibliography of materials published in the area of commercial radio and television engineering covers relevant areas as well as some described as peripheral including lighting, management, and microwave transmission theory. Reference materials are grouped alphabetically by case studies, circuits, current awareness services,…

  7. Concurrent engineering teams. Volume 2: Annotated bibliography

    Science.gov (United States)

    Richter, Karen J.; Dierolf, David A.

    1990-11-01

    Specific concurrent engineering practices vary among organizations. There are, however, various management practices that appear to work well for most organizations. This paper presents the reader with specific, useful examples from several defense contractors illustrating how multifunctional concurrent engineering teams are being organized and managed and how concurrent engineering team meetings are conducted and supported. The types of computer support that could be used to enhance the efficiency and effectiveness of concurrent engineering team meetings are identified. The general findings are that there exists a direct relationship between total quality management (TQM) and concurrent engineering, and that many applications of computer-aided group problem solving are possible and practical today for the concurrent engineering team meetings. Areas identified for additional research are the documentation of the decision process and rationale during the product and process definition, the capturing of lessons learned during the implementation of concurrent engineering, and the performance evaluation and training of team members.

  8. Concurrent Engineering Teams. Volume 2: Annotated Bibliography

    Science.gov (United States)

    1990-11-01

    Feigenbaum. A.V., Total Quality Control, McGraw-Hill Book Company, NY, 1983. Goldratt , Eliyahu and Jeff Cox, The Goal, A Process of Ongoing Improvement...design, namely concurrent engineering design for the life cycle. We introduce the emergent notion of Meta -Design, which we believe can be understood

  9. Bibliography

    NARCIS (Netherlands)

    NN,

    1995-01-01

    AGUIRRE-HUDSON, B. & P.A. WOLSELEY. 1994. Bibliography of South-east Asian Lichenology 1810-1992. J. Hattori Bot. Lab. 76: 313-350. GALLOWAY, D.J., M.W. SAMSUDIN & A. LATIFF. 1994. A bibliography of Malaysian lichenology. Mal. Appl. Biol. 22: 215-221.

  10. Bibliography

    Science.gov (United States)

    Darinsky, A. V.

    1976-01-01

    The bibliography provides 128 titles of printed materials related to educational development in the USSR, and the publishers' names (many in Russian). The cultural revolution, eradication of illiteracy, adults' general education, contemporary evening schools, textbooks, and statistical reference books, as well as bibliographies and adult education…

  11. Bibliographies.

    Science.gov (United States)

    Bourdon, Cathleen

    2001-01-01

    This selective bibliography of books for librarians includes titles regarding academic libraries; administration and personnel; bibliographic instruction; cataloging and classification; children's and young adult services; collection development; copyright; customer service; distance education; electronic libraries; fund raising; government…

  12. Bibliography of Connecticut Advanced Nuclear Engineering Laboratory reports

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1965-12-01

    This report, published in two, volumes, is a bibliography of the reports published at the Connecticut Advanced Nuclear Engineering Laboratory (CANEL). The reports cover the period 1952 through 1965 and include the Aircraft Nuclear Propulsion program, the Advanced Liquid Metal Cooled Reactor program, the Advanced Reactor Materials program and the SNAP-50 program. The bibliography contains the report number, title, author, date published, and classification. In some cases where the writing of a report was a group effort, and in some reports containing compilations of certain types of data, the author column is not applicable. This is indicated by a {open_quotes}n.a.{close_quotes} in the author column. The following types of reports are included: PWAC`s, TIM`s, CNLM`s. FXM`s and miscellaneous reports. PWAC and TIM reports conform to the requirements of AEC Manual Chapter 3202-041 and 3202-042, respectively. Most of the technical information of interest generated by this project is documented in these reports, CNLM and FXM reports were written primarily for internal distribution. However, these reports contain enough information of technical interest to warrant their inclusion. All CNLM`s and those FXM`s considered to be of interest are included in this bibliography. The MPR`s (Monthly Progress Reports) are the most important of the miscellaneous categories of reports. The other miscellaneous categories relate primarily to equipment and reactor specifications. The Division of Technical Information Extension (DTIE) at Oak Ridge, Tennessee has been designated as the primary recipient of the reports in the CANEL library. When more than one copy of a report was available, the additional copies were delivered to the Lawrence Radiation Laboratory, Livermore, California.

  13. Bibliography of Connecticut Advanced Nuclear Engineering Laboratory reports

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1965-12-01

    This report, published in two volumes, is a bibliography of the reports published at the Connecticut Advanced Nuclear Engineering Laboratory (CANEL). The reports cover the period 1952 through 1965 and include the Aircraft Nuclear Propulsion program, the Advanced Liquid Metal Cooled Reactor program, the Advanced Reactor Materials program and the SNAP-50 program. The bibliography contains the report number, title, author, date published, and classification. In some cases where the writing of a report was a group effort, and in some reports containing compilations of certain types of data, the author column is not applicable. This is indicated by a {open_quotes}n.a.{close_quotes} in the author column. The following types of reports are included: PWAC`s, TIM`s, CNLM`s, FXM`s and miscellaneous reports. PWAC and TIM reports conform to the requirements of AEC Manual Chapter 3202-041 and 3202-042, respectively. Most of the technical information of interest generated by this project is documented in these reports. CNLM and FXM reports were written primarily for internal distribution. However, these reports contain enough information of technical interest to warrant their inclusion. All CNLM`s and those FXM`s considered to be of interest are included in this bibliography. The MPR`s (Monthly Progress Reports) are the most important of the miscellaneous categories of reports. The other miscellaneous categories relate primarily to equipment and reactor specifications. The Division of Technical Information Extension (DTIE) at Oak Ridge, Tennessee has been designated as the primary recipient of the reports in the CANEL library. When more than one copy of a report was available, the additional copies were delivered to the Lawrence Radiation Laboratory, Livermore, California.

  14. Bibliographie

    OpenAIRE

    2016-01-01

    — Éditions des ouvrages de Doyle utilisées — La bibliographie la plus complète établie est celle de R.L. Green & J. M. Gibson, A Bibliography of Sir Arthur Conan Doyle, Oxford, Clarendon Press, 1983. On pourra également consulter : Green & Gibson, The Unknown Conan Doyle : Essays in Photography, London, Secker and Warburg, 1982 Green & Gibson, The Unknown Conan Doyle : Letters to the Press, London, Secker and Warburg, 1986 Green & Gibson, The Unknown Conan Doyle : Uncollected Stories, London,...

  15. Bibliographies.

    Science.gov (United States)

    Halsted, Ann L., Comp.

    1983-01-01

    Two annotated bibliographies of books are compiled, one devoted to Roosevelt and the New Deal, the other concerned with Hitler, the Weimar Republic, and the rise of Nazism. Annotations indicate scope of the work and occasionally point out bias or point of view. (CS)

  16. An Interdisciplinary Bibliography for Computers and the Humanities Courses.

    Science.gov (United States)

    Ehrlich, Heyward

    1991-01-01

    Presents an annotated bibliography of works related to the subject of computers and the humanities. Groups items into textbooks and overviews; introductions; human and computer languages; literary and linguistic analysis; artificial intelligence and robotics; social issue debates; computers' image in fiction; anthologies; writing and the…

  17. Social and Psychological Aspects of Applied Human Genetics: A Bibliography.

    Science.gov (United States)

    Sorenson, James R., Comp.

    This bibliography is a selective compilation of books and articles which focus on the psychological and social issues of applied human genetics. It is centered in particular around problems, issues, and discussions of genetic counseling, the primary mechanism by which human genetics has been applied to date. It includes those entries which, on the…

  18. Teacher-to-Teacher: An Annotated Bibliography on DNA and Genetic Engineering.

    Science.gov (United States)

    Mertens, Thomas R., Comp.

    1984-01-01

    Presented is an annotated bibliography of 24 books on DNA and genetic engineering. Areas considered in these books include: basic biological concepts to help understand advances in genetic engineering; applications of genetic engineering; social, legal, and moral issues of genetic engineering; and historical aspects leading to advances in…

  19. Genetic engineering possibilities for CELSS: A bibliography and summary of techniques

    Science.gov (United States)

    Johnson, E. J.

    1982-01-01

    A bibliography of the most useful techniques employed in genetic engineering of higher plants, bacteria associated with plants, and plant cell cultures is provided. A resume of state-of-the-art genetic engineering of plants and bacteria is presented. The potential application of plant bacterial genetic engineering to CELSS (Controlled Ecological Life Support System) program and future research needs are discussed.

  20. Software engineering laboratory series: Annotated bibliography of software engineering laboratory literature

    Science.gov (United States)

    Morusiewicz, Linda; Valett, Jon

    1992-01-01

    This document is an annotated bibliography of technical papers, documents, and memorandums produced by or related to the Software Engineering Laboratory. More than 100 publications are summarized. These publications cover many areas of software engineering and range from research reports to software documentation. This document has been updated and reorganized substantially since the original version (SEL-82-006, November 1982). All materials have been grouped into eight general subject areas for easy reference: (1) the Software Engineering Laboratory; (2) the Software Engineering Laboratory: Software Development Documents; (3) Software Tools; (4) Software Models; (5) Software Measurement; (6) Technology Evaluations; (7) Ada Technology; and (8) Data Collection. This document contains an index of these publications classified by individual author.

  1. Bibliographie (Bibliography).

    Science.gov (United States)

    Francais dans le Monde, 1981

    1981-01-01

    Presents a selective bibliography of dictionaries, books, and articles. References are grouped under three sections: I. Lexicology, II. Dictionaries and III. Didactics. Each item is followed by a concise critical note. The compilation is by the "Centre d'Etudes du Lexique" (CELEX, Paris-Villetaneuse). (MES)

  2. Homosexuality and Human Rights: A Selected Bibliography. Number 12.

    Science.gov (United States)

    Miller, Alan V., Comp.

    This bibliography represents the second in a series of three dealing with employment of homosexuals, to be published by the Ontario Ministry of Labour Library. All asterisked titles in the series deal with the employment problems of and discrimination against homosexuals. This bibliography of reading materials concerned with homosexuality and…

  3. Aeronautical Engineering: A Continuing Bibliography with Indexes (Supplement 216)

    Science.gov (United States)

    1987-08-01

    Eular Some considerations relatling to aero engine The terstetal eree sinviulaton system. Volume 2: approach p 415 N487-20217 pyromatt p 468 N467...afttiude dteO~noiwtn trom vector weettier channel an a contribtution to the modernization p 424 A87-32fl?0 obearvationa Eular , angle isiiiton p 444 A87

  4. ELSI Bibliography: Ethical legal and social implications of the Human Genome Project

    Energy Technology Data Exchange (ETDEWEB)

    Yesley, M.S. [comp.

    1993-11-01

    This second edition of the ELSI Bibliography provides a current and comprehensive resource for identifying publications on the major topics related to the ethical, legal and social issues (ELSI) of the Human Genome Project. Since the first edition of the ELSI Bibliography was printed last year, new publications and earlier ones identified by additional searching have doubled our computer database of ELSI publications to over 5600 entries. The second edition of the ELSI Bibliography reflects this growth of the underlying computer database. Researchers should note that an extensive collection of publications in the database is available for public use at the General Law Library of Los Alamos National Laboratory (LANL).

  5. Integrated Community Energy Systems: engineering analysis and design bibliography. [368 citations

    Energy Technology Data Exchange (ETDEWEB)

    Calm, J.M.; Sapienza, G.R.

    1979-05-01

    This bibliography cites 368 documents that may be helpful in the planning, analysis, and design of Integrated Community Energy Systems. It has been prepared for use primarily by engineers and others involved in the development and implementation of ICES concepts. These documents include products of a number of Government research, development, demonstration, and commercialization programs; selected studies and references from the literature of various technical societies and institutions; and other selected material. The key programs which have produced cited reports are the Department of Energy Community Systems Program (DOE/CSP), the Department of Housing and Urban Development Modular Integrated Utility Systems Program (HUD/MIUS), and the Department of Health, Education, and Welfare Integrated Utility Systems Program (HEW/IUS). The cited documents address experience gained both in the U.S. and in other countries. Several general engineering references and bibliographies pertaining to technologies or analytical methods that may be helpful in the analysis and design of ICES are also included. The body of relevant literature is rapidly growing and future updates are therefore planned. Each citation includes identifying information, a source, descriptive information, and an abstract. The citations are indexed both by subjects and authors, and the subject index is extensively cross-referenced to simplify its use.

  6. Medical devices and human engineering

    CERN Document Server

    Bronzino, Joseph D

    2014-01-01

    Known as the bible of biomedical engineering, The Biomedical Engineering Handbook, Fourth Edition, sets the standard against which all other references of this nature are measured. As such, it has served as a major resource for both skilled professionals and novices to biomedical engineering.Medical Devices and Human Engineering, the second volume of the handbook, presents material from respected scientists with diverse backgrounds in biomedical sensors, medical instrumentation and devices, human performance engineering, rehabilitation engineering, and clinical engineering.More than three doze

  7. Annotated Bibliography of the Air Force Human Resources Laboratory Technical Reports--1979. Final Report.

    Science.gov (United States)

    Barlow, Esther M.

    This annotated bibliography presents summaries of 81 reports on personnel and training research conducted by the Air Force Human Resources Laboratory (AFHRL). Topics addressed include electronics, aeronautics, computers, mathematics, and operational research, as they relate to the selection, motivation, training, retention, education, utilization,…

  8. Annotated Bibliography of the Air Force Human Resources Laboratory Technical Reports--1976.

    Science.gov (United States)

    Barlow, Esther M., Comp.

    This annotated bibliography presents a listing of technical reports (1976) dealing with personnel and training research conducted by the Air Force Human Resources Laboratory, an institution charged with the planning and execution of United States Air Force exploratory and advanced development programs for selection, motivation, training,…

  9. ELSI Bibliography: Ethical, legal and social implications of the Human Genome Project. 1994 Supplement

    Energy Technology Data Exchange (ETDEWEB)

    Yesley, M.S.; Ossorio, P.N. [comps.

    1994-09-01

    This report updates and expands the second edition of the ELSI Bibliography, published in 1993. The Bibliography and Supplement provides a comprehensive resource for identifying publications on the major topics related to the ethical, legal and social issues (ELSI) of the Human Genome Project. The Bibliography and Supplement are extracted from a database compiled at Los Alamos National Laboratory with the support of the Office of Energy Research, US Department of Energy. The second edition of the ELSI Bibliography was dated May 1993 but included publications added to the database until fall 1993. This Supplement reflects approximately 1,000 entries added to the database during the past year, bringing the total to approximately 7,000 entries. More than half of the new entries were published in the last year, and the remainder are earlier publications not previously included in the database. Most of the new entries were published in the academic and professional literature. The remainder are press reports from newspapers of record and scientific journals. The topical listing of the second edition has been followed in the Supplement, with a few changes. The topics of Cystic Fibrosis, Huntington`s Disease, and Sickle Cell Anemia have been combined in a single topic, Disorders. Also, all the entries published in the past year are included in a new topic, Publications: September 1993--September 1994, which provides a comprehensive view of recent reporting and commentary on the science and ELSI of genetics.

  10. Human Factors Plan for Maritime Safety: Annotated Bibliography

    Science.gov (United States)

    1993-02-01

    behavior. a Product description: Describes the features of sophisticated hardware or software for ships. H Regulation/Standard: Based on formal rules...required. The paper reports increasing industry support for mandatory practical training, especially related to automation and Hazops . Proposed CG...study would review deck and engine department functions, determine Hazops and special training needs. Also, this report suggests the need to evaluate

  11. Genetic Engineering

    Science.gov (United States)

    Phillips, John

    1973-01-01

    Presents a review of genetic engineering, in which the genotypes of plants and animals (including human genotypes) may be manipulated for the benefit of the human species. Discusses associated problems and solutions and provides an extensive bibliography of literature relating to genetic engineering. (JR)

  12. Software Engineering for Human Spaceflight

    Science.gov (United States)

    Fredrickson, Steven E.

    2014-01-01

    The Spacecraft Software Engineering Branch of NASA Johnson Space Center (JSC) provides world-class products, leadership, and technical expertise in software engineering, processes, technology, and systems management for human spaceflight. The branch contributes to major NASA programs (e.g. ISS, MPCV/Orion) with in-house software development and prime contractor oversight, and maintains the JSC Engineering Directorate CMMI rating for flight software development. Software engineering teams work with hardware developers, mission planners, and system operators to integrate flight vehicles, habitats, robotics, and other spacecraft elements. They seek to infuse automation and autonomy into missions, and apply new technologies to flight processor and computational architectures. This presentation will provide an overview of key software-related projects, software methodologies and tools, and technology pursuits of interest to the JSC Spacecraft Software Engineering Branch.

  13. Genome engineering in human cells.

    Science.gov (United States)

    Song, Minjung; Kim, Young-Hoon; Kim, Jin-Soo; Kim, Hyongbum

    2014-01-01

    Genome editing in human cells is of great value in research, medicine, and biotechnology. Programmable nucleases including zinc-finger nucleases, transcription activator-like effector nucleases, and RNA-guided engineered nucleases recognize a specific target sequence and make a double-strand break at that site, which can result in gene disruption, gene insertion, gene correction, or chromosomal rearrangements. The target sequence complexities of these programmable nucleases are higher than 3.2 mega base pairs, the size of the haploid human genome. Here, we briefly introduce the structure of the human genome and the characteristics of each programmable nuclease, and review their applications in human cells including pluripotent stem cells. In addition, we discuss various delivery methods for nucleases, programmable nickases, and enrichment of gene-edited human cells, all of which facilitate efficient and precise genome editing in human cells.

  14. Tissue Engineered Human Skin Equivalents

    Directory of Open Access Journals (Sweden)

    Zheng Zhang

    2012-01-01

    Full Text Available Human skin not only serves as an important barrier against the penetration of exogenous substances into the body, but also provides a potential avenue for the transport of functional active drugs/reagents/ingredients into the skin (topical delivery and/or the body (transdermal delivery. In the past three decades, research and development in human skin equivalents have advanced in parallel with those in tissue engineering and regenerative medicine. The human skin equivalents are used commercially as clinical skin substitutes and as models for permeation and toxicity screening. Several academic laboratories have developed their own human skin equivalent models and applied these models for studying skin permeation, corrosivity and irritation, compound toxicity, biochemistry, metabolism and cellular pharmacology. Various aspects of the state of the art of human skin equivalents are reviewed and discussed.

  15. Human Factors Analysis in Software Engineering

    Institute of Scientific and Technical Information of China (English)

    Xu Ren-zuo; Ma Ruo-feng; Liu Li-na; Xiong Zhong-wei

    2004-01-01

    The general human factors analysis analyzes human functions, effects and influence in a system. But in a narrow sense, it analyzes human influence upon the reliability of a system, it includes traditional human reliability analysis, human error analysis, man-machine interface analysis, human character analysis, and others. A software development project in software engineering is successful or not to be completely determined by human factors. In this paper, we discuss the human factors intensions, declare the importance of human factors analysis for software engineering by listed some instances. At last, we probe preliminarily into the mentality that a practitioner in software engineering should possess.

  16. History of the Gas Turbine Engine in the United States: Bibliography

    Science.gov (United States)

    1988-05-11

    Fuel Consumption is the Major Consideration." Interavia 31 (May 1976):419. Grangier , M. "SNECMA M-53 Status Report." Interavia 31 (May 1976):409...34 Flying 107 (November 1980):37. Garrison, P.; and Collins, R. L. "Dead Engine: Single; Engine Failure: Twin." Eying 106 (November 1980):48. Grangier ...the 90’s." Asian Defense Journal 8 (August 1981):76. Grangier , M. "Single-Engine or Light Twin? A Difficult Choice for the Buyer." Interavia 36

  17. Ethical Issues in Health Services: A Report and Annotated Bibliography.

    Science.gov (United States)

    Carmody, James

    This publication identifies, discusses, and lists areas for further research for five ethical issues related to health services: 1) the right to health care; 2) death and euthanasia; 3) human experimentation; 4) genetic engineering; and, 5) abortion. Following a discussion of each issue is a selected annotated bibliography covering the years 1967…

  18. Human Flesh Search Engine and Online Privacy.

    Science.gov (United States)

    Zhang, Yang; Gao, Hong

    2016-04-01

    Human flesh search engine can be a double-edged sword, bringing convenience on the one hand and leading to infringement of personal privacy on the other hand. This paper discusses the ethical problems brought about by the human flesh search engine, as well as possible solutions.

  19. Bridging Resilience Engineering and Human Reliability Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Ronald L. Boring

    2010-06-01

    There has been strong interest in the new and emerging field called resilience engineering. This field has been quick to align itself with many existing safety disciplines, but it has also distanced itself from the field of human reliability analysis. To date, the discussion has been somewhat one-sided, with much discussion about the new insights afforded by resilience engineering. This paper presents an attempt to address resilience engineering from the perspective of human reliability analysis (HRA). It is argued that HRA shares much in common with resilience engineering and that, in fact, it can help strengthen nascent ideas in resilience engineering. This paper seeks to clarify and ultimately refute the arguments that have served to divide HRA and resilience engineering.

  20. Wind energy bibliography

    Energy Technology Data Exchange (ETDEWEB)

    None

    1995-05-01

    This bibliography is designed to help the reader search for information on wind energy. The bibliography is intended to help several audiences, including engineers and scientists who may be unfamiliar with a particular aspect of wind energy, university researchers who are interested in this field, manufacturers who want to learn more about specific wind topics, and librarians who provide information to their clients. Topics covered range from the history of wind energy use to advanced wind turbine design. References for wind energy economics, the wind energy resource, and environmental and institutional issues related to wind energy are also included.

  1. Vitrification publication bibliography

    Energy Technology Data Exchange (ETDEWEB)

    Schmieman, E.; Johns, W.E.

    1996-02-01

    This document was compiled by a group of about 12 graduate students in the Department of Mechanical Engineering and Material Science at Washington State University and was funded by the U.S. Department of Energy. The literature search resulting in the compilation of this bibliography was designed to be an exhaustive search for research and development work involving the vitrification of mixed wastes, published by domestic and foreign researchers, primarily during 1989-1994. The search techniques were dominated by electronic methods and this bibliography is also available in electronic format, Windows Reference Manager.

  2. Bibliography on La Mujer Chicana.

    Science.gov (United States)

    Gutierrez, Lewis A.

    Compiled from materials obtained by the Chicana Library Project, this bibliography cites 186 books, texts, articles, clippings, and special editions on the Chicana. Published between 1959 and 1974, the materials are available through the Human Resources Center. The materials are listed under the following subject headings: art, bibliographies,…

  3. 130 FEMINISM AND HUMAN GENETIC ENGINEERING: A ...

    African Journals Online (AJOL)

    Ike Odimegwu

    Abstract. Human genetic in the area of Bio-ethics is a new, rapidly advancing. Science. ... Human genetic engineering, a recent one in medical science and practice, is one ..... The Church on Cloning and Stem Cell Research. The teaching of ...

  4. Human Factors Engineering. Student Supplement,

    Science.gov (United States)

    1981-08-01

    Psychologists as Division 21 of the APA . It seems fitting, now that engineering psychology has been recognized as a viable entity, that we examine this new...34INFIC 161ST SCALE ___________ P CIUUIOIC POP40 3 SMTCPWONSI OARIA C0" NORMA . IN. AIDS AMU Ls". PLA0D 11aw Lu". FILM PACK CLR so. RAW MOTIVE LIS mm’ 7

  5. Human Factors Interface with Systems Engineering for NASA Human Spaceflights

    Science.gov (United States)

    Wong, Douglas T.

    2009-01-01

    This paper summarizes the past and present successes of the Habitability and Human Factors Branch (HHFB) at NASA Johnson Space Center s Space Life Sciences Directorate (SLSD) in including the Human-As-A-System (HAAS) model in many NASA programs and what steps to be taken to integrate the Human-Centered Design Philosophy (HCDP) into NASA s Systems Engineering (SE) process. The HAAS model stresses systems are ultimately designed for the humans; the humans should therefore be considered as a system within the systems. Therefore, the model places strong emphasis on human factors engineering. Since 1987, the HHFB has been engaging with many major NASA programs with much success. The HHFB helped create the NASA Standard 3000 (a human factors engineering practice guide) and the Human Systems Integration Requirements document. These efforts resulted in the HAAS model being included in many NASA programs. As an example, the HAAS model has been successfully introduced into the programmatic and systems engineering structures of the International Space Station Program (ISSP). Success in the ISSP caused other NASA programs to recognize the importance of the HAAS concept. Also due to this success, the HHFB helped update NASA s Systems Engineering Handbook in December 2007 to include HAAS as a recommended practice. Nonetheless, the HAAS model has yet to become an integral part of the NASA SE process. Besides continuing in integrating HAAS into current and future NASA programs, the HHFB will investigate incorporating the Human-Centered Design Philosophy (HCDP) into the NASA SE Handbook. The HCDP goes further than the HAAS model by emphasizing a holistic and iterative human-centered systems design concept.

  6. ESL Bibliography.

    Science.gov (United States)

    Greis, Naguib

    This is an 117-item bibliography for materials in English as a Second Language especially at college level. The materials are classified under the following categories: I Oral Skills, II Reading, Patterns and Vocabulary, III Writing and Grammar Exercises, IV Orientation, Library and Study Skills, V Technical English and VI Tests. (Author)

  7. Hydrogen Bibliography

    Energy Technology Data Exchange (ETDEWEB)

    1991-12-01

    The Hydrogen Bibliography is a compilation of research reports that are the result of research funded over the last fifteen years. In addition, other documents have been added. All cited reports are contained in the National Renewable Energy Laboratory (NREL) Hydrogen Program Library.

  8. Human Factors Engineering Program Review Model

    Science.gov (United States)

    2004-02-01

    AA NUREG -0711,Rev. 2 Human Factors Engineering Program Review Model 20081009191 I i m To] Bi U.S. Nuclear Regulatory Commission Office of...Material As of November 1999, you may electronically access NUREG -series publications and other NRC records at NRC’s Public Electronic Reading Room at...http://www.nrc.qov/readinq-rm.html. Publicly released records include, to name a few, NUREG -series publications; Federal Register notices; applicant

  9. Rapid Prototyping and the Human Factors Engineering Process

    Science.gov (United States)

    2016-08-29

    Rapid prototyping and the human factors • • engineering process David Beevis* and Gaetan St Denist *Senior Human Factors Engineer , Defence and...factors engineering analyses. Therefore, an investigation of the use of the V APS virtual prototyping system was carried out in five organizations. The...factors engineering (HFE) process re- commended for the development of human-machine systems is based on a series of increasin¥ly detailed analyses of

  10. Human subject research for engineers a practical guide

    CERN Document Server

    de Winter, Joost C F

    2017-01-01

    This Brief introduces engineers to the main principles in ethics, research design, statistics, and publishing of human subject research. In recent years, engineering has become strongly connected to disciplines such as biology, medicine, and psychology. Often, engineers (and engineering students) are expected to perform human subject research. Typical human subject research topics conducted by engineers include human-computer interaction (e.g., evaluating the usability of software), exoskeletons, virtual reality, teleoperation, modelling of human behaviour and decision making (often within the framework of ‘big data’ research), product evaluation, biometrics, behavioural tracking (e.g., of work and travel patterns, or mobile phone use), transport and planning (e.g., an analysis of flows or safety issues), etc. Thus, it can be said that knowledge on how to do human subject research is indispensable for a substantial portion of engineers. Engineers are generally well trained in calculus and mechanics, but m...

  11. The Humanities: Resources and Information Systems. Outline and Bibliography. Bibliographic Studies Number Two.

    Science.gov (United States)

    Lemke, Antje B.

    The contents of this course outline on resources and information in the humanities are mainly bibliographic. The subjects covered are: humanities in general; religion and theology; philosophy; visual arts, including archaeology, architecture, and photography; music; and literature and linguistics. The scope of the subject is outlined for each…

  12. Human Factors Engineering and Ergonomics in Systems Engineering

    Science.gov (United States)

    Whitmore, Mihriban

    2017-01-01

    The study, discovery, and application of information about human abilities, human limitations, and other human characteristics to the design of tools, devices, machines, systems, job tasks and environments for effective human performance.

  13. Human-Centered Software Engineering: Software Engineering Architectures, Patterns, and Sodels for Human Computer Interaction

    Science.gov (United States)

    Seffah, Ahmed; Vanderdonckt, Jean; Desmarais, Michel C.

    The Computer-Human Interaction and Software Engineering (CHISE) series of edited volumes originated from a number of workshops and discussions over the latest research and developments in the field of Human Computer Interaction (HCI) and Software Engineering (SE) integration, convergence and cross-pollination. A first volume in this series (CHISE Volume I - Human-Centered Software Engineering: Integrating Usability in the Development Lifecycle) aims at bridging the gap between the field of SE and HCI, and addresses specifically the concerns of integrating usability and user-centered systems design methods and tools into the software development lifecycle and practices. This has been done by defining techniques, tools and practices that can fit into the entire software engineering lifecycle as well as by defining ways of addressing the knowledge and skills needed, and the attitudes and basic values that a user-centered development methodology requires. The first volume has been edited as Vol. 8 in the Springer HCI Series (Seffah, Gulliksen and Desmarais, 2005).

  14. Human factors engineering program review model

    Energy Technology Data Exchange (ETDEWEB)

    1994-07-01

    The staff of the Nuclear Regulatory Commission is performing nuclear power plant design certification reviews based on a design process plan that describes the human factors engineering (HFE) program elements that are necessary and sufficient to develop an acceptable detailed design specification and an acceptable implemented design. There are two principal reasons for this approach. First, the initial design certification applications submitted for staff review did not include detailed design information. Second, since human performance literature and industry experiences have shown that many significant human factors issues arise early in the design process, review of the design process activities and results is important to the evaluation of an overall design. However, current regulations and guidance documents do not address the criteria for design process review. Therefore, the HFE Program Review Model (HFE PRM) was developed as a basis for performing design certification reviews that include design process evaluations as well as review of the final design. A central tenet of the HFE PRM is that the HFE aspects of the plant should be developed, designed, and evaluated on the basis of a structured top-down system analysis using accepted HFE principles. The HFE PRM consists of ten component elements. Each element in divided into four sections: Background, Objective, Applicant Submittals, and Review Criteria. This report describes the development of the HFE PRM and gives a detailed description of each HFE review element.

  15. Annotated Bibliography of the Air Force Human Resources Laboratory Technical Reports - 1978

    Science.gov (United States)

    1980-06-01

    listing of technical reports (I 978 dligwtIernnladri- ig research condutted hv the Air Force Human Resources Laborator)6 (AFIIRI,). The research has been...geometry configuration: and other lesser problems. such as vibration, thermal . accoustic. and ghosts. A 17-inch aperture. holographic volume-phase. on-axis

  16. Engineered human dicentric chromosomes show centromere plasticity.

    Science.gov (United States)

    Higgins, Anne W; Gustashaw, Karen M; Willard, Huntington F

    2005-01-01

    The centromere is essential for the faithful distribution of a cell's genetic material to subsequent generations. Despite intense scrutiny, the precise genetic and epigenetic basis for centromere function is still unknown. Here, we have used engineered dicentric human chromosomes to investigate mammalian centromere structure and function. We describe three classes of dicentric chromosomes isolated in different cell lines: functionally monocentric chromosomes, in which one of the two genetically identical centromeres is consistently inactivated; functionally dicentric chromosomes, in which both centromeres are consistently active; and dicentric chromosomes heterogeneous with respect to centromere activity. A study of serial single cell clones from heterogeneous cell lines revealed that while centromere activity is usually clonal, the centromere state (i.e. functionally monocentric or dicentric) in some lines can switch within a growing population of cells. Because pulsed field gel analysis indicated that the DNA at the centromeres of these chromosomes did not change detectably, this switching of the centromere state is most likely due to epigenetic changes. Inactivation of one of the two active centromeres in a functionally dicentric chromosome was observed in a percentage of cells after treatment with Trichostatin A, an inhibitor of histone deacetylation. This study provides evidence that the activity of human centromeres, while largely stable, can be subject to dynamic change, most likely due to epigenetic modification.

  17. Human Performance: Women in Nontraditional Occupations and the Influence of the Menstrual Cycle (A Selected Bibliography)

    Science.gov (United States)

    1980-05-01

    Physiological, medical, epidemiological and psychological studies. New York: New York Academy of Sciences, 1977. 16. Morris , P. C. A comparative study... Degroot , L. J., Cahill, G. F., Odell, W. D., Martini, L., Potts, J. T., Nelson, D. H., Steinberger, E., & Winegtad, A. I. (Eds.). Endocrinology (Vol. 3...Variation of the human menstrual cycle through reproductive life. International Journal of Fertility, 1967, 12, 77-126. 47. Udrey, J. R., & Morris , N

  18. Human Genetic Engineering: A Survey of Student Value Stances

    Science.gov (United States)

    Wilson, Sara McCormack; And Others

    1975-01-01

    Assesses the values of high school and college students relative to human genetic engineering and recommends that biology educators explore instructional strategies merging human genetic information with value clarification techniques. (LS)

  19. RNA-guided human genome engineering via Cas9

    National Research Council Canada - National Science Library

    Mali, Prashant; Yang, Luhan; Esvelt, Kevin M; Aach, John; Guell, Marc; DiCarlo, James E; Norville, Julie E; Church, George M

    2013-01-01

    .... We also compute a genome-wide resource of ~190 K unique gRNAs targeting ~40.5% of human exons. Our results establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.

  20. A systems engineering view of the human in space

    Science.gov (United States)

    Anderson, John L.

    1987-01-01

    A model of the human as an 'engineered' system provides a starting point for determining human requirements and performance on an equivalent basis with technological systems. The human as an engineered system with performance requirements is defined to consist of four subsystems: cognitive, psychological, biomechanical, and biomedical. It is suggested that the treatment of the psychological subsystem as one that modulates the efficiency and quality of human performance offers a particular approach for examining and characterizing psychological effects.

  1. Bibliography of Research Reports and Publications Issued by the Human Engineering Division, January 1987 - December 1993

    Science.gov (United States)

    1994-03-01

    participatory ergonomics. The International Journal of Industrial Ergonomics . McNeese, M. D., Zaff, B. S., Peio, K. J., Snyder, D. E., Duncan, J. C...ergonomics. The International Journal of Industrial Ergonomics . McNeese, M. D., Zaff, B. S., Peio, K. J., Snyder, D. E., Duncan, J. C., & McFarren, M. R

  2. Local control stations: Human engineering issues and insights

    Energy Technology Data Exchange (ETDEWEB)

    Brown, W.S.; Higgins, J.C.; O`Hara, J.M. [Brookhaven National Lab., Upton, NY (United States)

    1994-09-01

    The objective of this research project was to evaluate current human engineering at local control stations (LCSs) in nuclear power plants, and to identify good human engineering practices relevant to the design of these operator interfaces. General literature and reports of operating experience were reviewed to determine the extent and type of human engineering deficiencies at LCSs in nuclear power plants. In-plant assessments were made of human engineering at single-function as well as multifunction LCSs. Besides confirming the existence of human engineering deficiencies at LCSs, the in-plant assessments provided information about the human engineering upgrades that have been made at nuclear power plants. Upgrades were typically the result of any of three influences regulatory activity, broad industry initiatives such as INPO, and specific in-plant programs (e.g. activities related to training). It is concluded that the quality of LCSs is quite variable and might be improved if there were greater awareness of good practices and existing human engineering guidance relevant to these operator interfaces, which is available from a variety of sources. To make such human engineering guidance more readily accessible, guidelines were compiled from such sources and included in the report as an appendix.

  3. Recent progress in engineering human-associated microbiomes.

    Science.gov (United States)

    Yaung, Stephanie J; Church, George M; Wang, Harris H

    2014-01-01

    Recent progress in molecular biology and genetics opens up the possibility of engineering a variety of biological systems, from single-cellular to multicellular organisms. The consortia of microbes that reside on the human body, the human-associated microbiota, are particularly interesting as targets for forward engineering and manipulation due to their relevance in health and disease. New technologies in analysis and perturbation of the human microbiota will lead to better diagnostic and therapeutic strategies against diseases of microbial origin or pathogenesis. Here, we discuss recent advances that are bringing us closer to realizing the true potential of an engineered human-associated microbial community.

  4. Chernobyl bibliography

    Energy Technology Data Exchange (ETDEWEB)

    Carr, F. Jr.; Mahaffey, J.A.

    1989-09-01

    The purpose of the DOE/OHER Chernobyl Database project is to create and maintain an information system to provide usable information for research studies related to the nuclear accident. The system is the official United States repository for information about the Chernobyl accident and its consequences, and currently includes an extensive bibliography and diverse radiological measurements with supporting information. PNL has established two resources: original (not summarized) measurement data, currently about 80,000 measurements, with ancillary information; and about 2,200 bibliographic citations, some including abstracts. Major organizations that have contributed radiological measurement data include the Washington State Department of Social and Health Services; United States Environmental Protection Agency (domestic and foreign data); United States Nuclear Regulatory Commission; Stone Webster; Brookhaven National Laboratory; Commissariat A L'energie Atomique in France; Ministry of Agriculture, Fisheries, and Food in the United Kingdom; Japan National Institute of Radiological Sciences; and the Finnish Centre For Radiation and Nuclear Safety (STUK). Scientists in Australia, Austria, Belgium, Canada, China, Denmark, England, Federal Republic of Germany, Finland, France, Ireland, Italy, Japan, the Netherlands, Romania, Scotland, Spain, Sweden, Switzerland, United States, Wales, and Yugoslavia have made contributions. Bibliographic materials have been obtained from scientists in the above countries that have replied to requests. In addition, literature searches have been conducted, including a search of the DOE Energy Database. The last search was conducted in January, 1989. This document lists the bibliographic information in the DOE/OHER Chernobyl Database at the current time.

  5. Human Engineering Modeling and Performance Lab Study Project

    Science.gov (United States)

    Oliva-Buisson, Yvette J.

    2014-01-01

    The HEMAP (Human Engineering Modeling and Performance) Lab is a joint effort between the Industrial and Human Engineering group and the KAVE (Kennedy Advanced Visualiations Environment) group. The lab consists of sixteen camera system that is used to capture human motions and operational tasks, through te use of a Velcro suit equipped with sensors, and then simulate these tasks in an ergonomic software package know as Jac, The Jack software is able to identify the potential risk hazards.

  6. Prison Libraries: Bibliography.

    Science.gov (United States)

    Gillespie, David M.

    An Alphabetically arranged bibliography, listing 485 entries representing 518 citations taken from "Poole's Index to Periodical Literature" 1802-1906, "Cannon's Bibliography of Library Economy," and "Library Literature" 1921 to mid-1970. Other sources used include: The Index to the Journal of Correctional Education; ten bibliographies taken from…

  7. Seeking perfection: a Kantian look at human genetic engineering.

    Science.gov (United States)

    Gunderson, Martin

    2007-01-01

    It is tempting to argue that Kantian moral philosophy justifies prohibiting both human germ-line genetic engineering and non-therapeutic genetic engineering because they fail to respect human dignity. There are, however, good reasons for resisting this temptation. In fact, Kant's moral philosophy provides reasons that support genetic engineering-even germ-line and non-therapeutic. This is true of Kant's imperfect duties to seek one's own perfection and the happiness of others. It is also true of the categorical imperative. Kant's moral philosophy does, however, provide limits to justifiable genetic engineering.

  8. Unmasking the social engineer the human element of security

    CERN Document Server

    Hadnagy, Christopher

    2014-01-01

    Learn to identify the social engineer by non-verbal behavior Unmasking the Social Engineer: The Human Element of Security focuses on combining the science of understanding non-verbal communications with the knowledge of how social engineers, scam artists and con men use these skills to build feelings of trust and rapport in their targets. The author helps readers understand how to identify and detect social engineers and scammers by analyzing their non-verbal behavior. Unmasking the Social Engineer shows how attacks work, explains nonverbal communications, and demonstrates with visuals the c

  9. Urban Sociology Bibliography, Exchange Bibliography 1336.

    Science.gov (United States)

    Wood, James L.; And Others

    This bibliography on urban sociology cites studies which discuss the structures and processes of urban society from an historical and comparative perspective. Although it includes studies that discuss population growth, decline, size, and density, the prime concern of this bibliography is not with urban demography. After a brief look at some…

  10. Adaptation to prolonged bedrest in man: A compendium of research. [bibliographies on clinical medicine and human pathology

    Science.gov (United States)

    Greenleaf, J. E.; Greenleaf, C. J.; Vanderveer, D.; Dorchak, K. J.

    1976-01-01

    A compilation of major studies that describe the clinical observations and elucidate the physiological mechanisms of the adaptive process of man undergoing prolonged bed rest is presented. Additional studies are included that provide background information in the form of reviews or summaries of the process. Wherever possible a detailed annotation is provided under the subheadings: (1) purpose, (2) procedure and methods, (3) results, and (4) conclusions. Additional references are provided in a selected bibliography.

  11. Human factor engineering applied to nuclear power plant design

    Energy Technology Data Exchange (ETDEWEB)

    Manrique, A. [TECNATOM SA, BWR General Electric Business Manager, Madrid (Spain); Valdivia, J.C. [TECNATOM SA, Operation Engineering Project Manager, Madrid (Spain); Jimenez, A. [TECNATOM SA, Operation Engineering Div. Manager, Madrid (Spain)

    2001-07-01

    For the design and construction of new nuclear power plants as well as for maintenance and operation of the existing ones new man-machine interface designs and modifications are been produced. For these new designs Human Factor Engineering must be applied the same as for any other traditional engineering discipline. Advantages of implementing adequate Human Factor Engineering techniques in the design of nuclear reactors have become not only a fact recognized by the majority of engineers and operators but also an explicit requirement regulated and mandatory for the new designs of the so called advanced reactors. Additionally, the big saving achieved by a nuclear power plant having an operating methodology which significantly decreases the risk of operating errors makes it necessary and almost vital its implementation. The first step for this is preparing a plan to incorporate all the Human Factor Engineering principles and developing an integral design of the Instrumentation and Control and Man-machine interface systems. (author)

  12. Design of a Human Reliability Assessment model for structural engineering

    NARCIS (Netherlands)

    De Haan, J.; Terwel, K.C.; Al-Jibouri, S.H.S.

    2013-01-01

    It is generally accepted that humans are the “weakest link” in structural design and construction processes. Despite this, few models are available to quantify human error within engineering processes. This paper demonstrates the use of a quantitative Human Reliability Assessment model within struct

  13. Inspiring engineering minds to advance human health: the Henry Samueli School of Engineering's Department of BME.

    Science.gov (United States)

    Lee, Abraham; Wirtanen, Erik

    2012-07-01

    The growth of biomedical engineering at The Henry Samueli School of Engineering at the University of California, Irvine (UCI) has been rapid since the Center for Biomedical Engineering was first formed in 1998 [and was later renamed as the Department of Biomedical Engineering (BME) in 2002]. Our current mission statement, “Inspiring Engineering Minds to Advance Human Health,” serves as a reminder of why we exist, what we do, and the core principles that we value and by which we abide. BME exists to advance the state of human health via engineering innovation and practices. To attain our goal, we are empowering our faculty to inspire and mobilize our students to address health problems. We treasure the human being, particularly the human mind and health. We believe that BME is where minds are nurtured, challenged, and disciplined, and it is also where the health of the human is held as a core mission value that deserves our utmost priority (Figure 1). Advancing human health is not a theoretical practice; it requires bridging between disciplines (engineering and medicine) and between communities (academic and industry).

  14. Archaeological mounds as analogs of engineered covers for waste disposal sites: Literature review and progress report. [Appendix contains bibliography and data on archaeological mounds

    Energy Technology Data Exchange (ETDEWEB)

    Chatters, J C; Gard, H A

    1991-09-01

    Closure caps for low-level radioactive waste disposal facilities are typically designed as layered earthen structures, the composition of which is intended to prevent the infiltration of water and the intrusion of the public into waste forms. Federal regulations require that closure caps perform these functions well enough that minimum exposure guidelines will be met for at least 500 years. Short-term experimentation cannot mimic the conditions that will affect closure caps on the scale of centuries, and therefore cannot provide data on the performance of cap designs over long periods of time. Archaeological mounds hundreds to thousands of years old which are closely analogous to closure caps in form, construction details, and intent can be studied to obtain the necessary understanding of design performance. Pacific Northwest Laboratory conducted a review and analysis of archaeological literature on ancient human-made mounds to determine the quality and potential applicability of this information base to assessments of waste facility design performance. A bibliography of over 200 English-language references was assembled on mound structures from the Americas, Europe, and Asia. A sample of these texts was read for data on variables including environmental and geographic setting, condition, design features, construction. Detailed information was obtained on all variables except those relating to physical and hydrological characteristics of the mound matrix, which few texts presented. It is concluded that an extensive amount of literature and data are available on structures closely analogous to closure caps and that this information is a valuable source of data on the long-term performance of mounded structures. Additional study is recommended, including an expanded analysis of design features reported in the literature and field studies of the physical and hydraulic characteristics of different mound designs. 23 refs., 10 figs., 12 tabs.

  15. Requirements engineering for human activity systems

    CSIR Research Space (South Africa)

    Erasmus, J

    2014-10-01

    Full Text Available . Office of the Chief Engineer, NASA, 2007. —. Systems Engineering Handbook. Washington, D.C.: National Aeronautics and Space Administration, 2007. Office of the Deputy Under Secretary of Defense for Acquisition and Technology, Systems and Software... and special tools necessary to perform the verification (NASA 2007). This information will also allow for proper planning and scheduling of the verification activities to be performed throughout the development and realisation of the product. Measures...

  16. Cooperative and human aspects of software engineering: CHASE 2010

    DEFF Research Database (Denmark)

    Dittrich, Yvonne; Sharp, Helen C.; Winschiers Theophilus, Heike

    2010-01-01

    Software is created by people -- software engineers in cooperation with domain experts, users and other stakeholders--in varied environments, under various conditions. Thus understanding cooperative and human aspects of software development is crucial to comprehend how and which methods and tools...... are required, to improve the creation and maintenance of software. The 3rd workshop on Cooperative and Human Aspects of Software Engineering held at the International Conference on Software Engineering continued the tradition from earlier workshops and provided a lively forum to discuss current developments...... and high quality research in the field. Further dissemination of research results will lead to an improvement of software development and deployment across the globe....

  17. "Human Nature": Chemical Engineering Students' Ideas about Human Relationships with the Natural World

    Science.gov (United States)

    Goldman, Daphne; Assaraf, Orit Ben-Zvi; Shemesh, Julia

    2014-01-01

    While importance of environmental ethics, as a component of sustainable development, in preparing engineers is widely acknowledged, little research has addressed chemical engineers' environmental concerns. This study aimed to address this void by exploring chemical engineering students' values regarding human-nature relationships. The…

  18. "Human Nature": Chemical Engineering Students' Ideas about Human Relationships with the Natural World

    Science.gov (United States)

    Goldman, Daphne; Assaraf, Orit Ben-Zvi; Shemesh, Julia

    2014-01-01

    While importance of environmental ethics, as a component of sustainable development, in preparing engineers is widely acknowledged, little research has addressed chemical engineers' environmental concerns. This study aimed to address this void by exploring chemical engineering students' values regarding human-nature relationships. The study was…

  19. Genetic engineering of human pluripotent cells using TALE nucleases.

    Science.gov (United States)

    Hockemeyer, Dirk; Wang, Haoyi; Kiani, Samira; Lai, Christine S; Gao, Qing; Cassady, John P; Cost, Gregory J; Zhang, Lei; Santiago, Yolanda; Miller, Jeffrey C; Zeitler, Bryan; Cherone, Jennifer M; Meng, Xiangdong; Hinkley, Sarah J; Rebar, Edward J; Gregory, Philip D; Urnov, Fyodor D; Jaenisch, Rudolf

    2011-07-07

    Targeted genetic engineering of human pluripotent cells is a prerequisite for exploiting their full potential. Such genetic manipulations can be achieved using site-specific nucleases. Here we engineered transcription activator-like effector nucleases (TALENs) for five distinct genomic loci. At all loci tested we obtained human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) clones carrying transgenic cassettes solely at the TALEN-specified location. Our data suggest that TALENs employing the specific architectures described here mediate site-specific genome modification in human pluripotent cells with similar efficiency and precision as do zinc-finger nucleases (ZFNs).

  20. Buried waste integrated demonstration human engineered control station. Final report

    Energy Technology Data Exchange (ETDEWEB)

    1994-09-01

    This document describes the Human Engineered Control Station (HECS) project activities including the conceptual designs. The purpose of the HECS is to enhance the effectiveness and efficiency of remote retrieval by providing an integrated remote control station. The HECS integrates human capabilities, limitations, and expectations into the design to reduce the potential for human error, provides an easy system to learn and operate, provides an increased productivity, and reduces the ultimate investment in training. The overall HECS consists of the technology interface stations, supporting engineering aids, platform (trailer), communications network (broadband system), and collision avoidance system.

  1. Human Factors Engineering Aspects of Modifications in Control Room Modernization

    Energy Technology Data Exchange (ETDEWEB)

    Hugo, Jacques; Clefton, Gordon; Joe, Jeffrey

    2017-06-01

    This report describes the basic aspects of control room modernization projects in the U.S. nuclear industry and the need for supplementary guidance on the integration of human factors considerations into the licensing and regulatory aspects of digital upgrades. The report pays specific attention to the integration of principles described in NUREG-0711 (Human Factors Engineering Program Review Model) and how supplementary guidance can help to raise general awareness in the industry regarding the complexities of control room modernization projects created by many interdependent regulations, standards and guidelines. The report also describes how human factors engineering principles and methods provided by various resources and international standards can help in navigating through the process of licensing digital upgrades. In particular, the integration of human factors engineering guidance and requirements into the process of licensing digital upgrades can help reduce uncertainty related to development of technical bases for digital upgrades that will avoid the introduction of new failure modes.

  2. Solar ponds: a selected bibliography

    Energy Technology Data Exchange (ETDEWEB)

    1981-11-01

    This bibliography contains citations on: regular solar ponds; shallow solar ponds; and patents. Certain references are specifically recommended. The data bases searched for the bibliography are listed. (LEW)

  3. Studies of Scientific Disciplines. An Annotated Bibliography.

    Science.gov (United States)

    Weisz, Diane; Kruytbosch, Carlos

    Provided in this bibliography are annotated lists of social studies of science literature, arranged alphabetically by author in 13 disciplinary areas. These areas include astronomy; general biology; biochemistry and molecular biology; biomedicine; chemistry; earth and space sciences; economics; engineering; mathematics; physics; political science;…

  4. Advanced Human Factors Engineering Tool Technologies.

    Science.gov (United States)

    1987-03-20

    the mail and telephone surveys. The authors would also like to extend a special thanks to Mr. David Rose of the Naval Air Development Center for his...ADVANCED NUNAN FACTORS ENGINEERING TOOL TECHNOLOGIES 3/3 (U) CARLON ASSOCIATES INC FAIRFAX Yff S A FLEGER ET AL. UNCLRS 20 NAR B? DARI5-BS-C-NO64 WIL...34" ".--: :’-...2,,. ,..:,.- ,’-"-’:"- "’-::"-,2 ., ..,," ,.- ..’.-.-.’.-,-. : .....v. _ *’--..., ...-- ,,. - -.; , :¢ 4., 5 5 lPeter laines Mr. David M. Ilarrah

  5. Cooperative and human aspects of software engineering: CHASE 2010

    DEFF Research Database (Denmark)

    Dittrich, Yvonne; Sharp, Helen C.; Winschiers Theophilus, Heike;

    2010-01-01

    are required, to improve the creation and maintenance of software. The 3rd workshop on Cooperative and Human Aspects of Software Engineering held at the International Conference on Software Engineering continued the tradition from earlier workshops and provided a lively forum to discuss current developments...... and high quality research in the field. Further dissemination of research results will lead to an improvement of software development and deployment across the globe....

  6. Engineering

    National Research Council Canada - National Science Library

    Includes papers in the following fields: Aerospace Engineering, Agricultural Engineering, Chemical Engineering, Civil Engineering, Electrical Engineering, Environmental Engineering, Industrial Engineering, Materials Engineering, Mechanical...

  7. Cooperative and Human Aspects of Software Engineering (CHASE 2008)

    DEFF Research Database (Denmark)

    2008-01-01

    The CHASE 2008 workshop is concerned with exploring the cooperative and human aspects of software engineering, and providing a forum for discussing high-quality research. Accepted papers reflect diversity of the field of software engineering – ranging from requirements to testing, and from...... ethnographic research to experiments. Moreover, the background of attendees reflects the diversity of researchers in this domain, ranging from sociology to psychology, from informatics to software engineering. CHASE 1008 met its goals in presenting high-quality research and building community through a mixture...

  8. Cooperative and Human Aspects of Software Engineering (CHASE 2008)

    DEFF Research Database (Denmark)

    2008-01-01

    The CHASE 2008 workshop is concerned with exploring the cooperative and human aspects of software engineering, and providing a forum for discussing high-quality research. Accepted papers reflect diversity of the field of software engineering – ranging from requirements to testing, and from...... ethnographic research to experiments. Moreover, the background of attendees reflects the diversity of researchers in this domain, ranging from sociology to psychology, from informatics to software engineering. CHASE 1008 met its goals in presenting high-quality research and building community through a mixture...

  9. Plant-Derived Human Collagen Scaffolds for Skin Tissue Engineering

    Science.gov (United States)

    Willard, James J.; Drexler, Jason W.; Das, Amitava; Roy, Sashwati; Shilo, Shani; Shoseyov, Oded

    2013-01-01

    Tissue engineering scaffolds are commonly formed using proteins extracted from animal tissues, such as bovine hide. Risks associated with the use of these materials include hypersensitivity and pathogenic contamination. Human-derived proteins lower the risk of hypersensitivity, but possess the risk of disease transmission. Methods engineering recombinant human proteins using plant material provide an alternate source of these materials without the risk of disease transmission or concerns regarding variability. To investigate the utility of plant-derived human collagen (PDHC) in the development of engineered skin (ES), PDHC and bovine hide collagen were formed into tissue engineering scaffolds using electrospinning or freeze-drying. Both raw materials were easily formed into two common scaffold types, electrospun nonwoven scaffolds and lyophilized sponges, with similar architectures. The processing time, however, was significantly lower with PDHC. PDHC scaffolds supported primary human cell attachment and proliferation at an equivalent or higher level than the bovine material. Interleukin-1 beta production was significantly lower when activated THP-1 macrophages where exposed to PDHC electrospun scaffolds compared to bovine collagen. Both materials promoted proper maturation and differentiation of ES. These data suggest that PDHC may provide a novel source of raw material for tissue engineering with low risk of allergic response or disease transmission. PMID:23298216

  10. PR Bibliography, 2002.

    Science.gov (United States)

    Ramsey, Shirley, Ed.

    2002-01-01

    This annotated bibliography presents an overview of journal articles and books published in 2001 on public relations that can be helpful to teachers and students as well as to practitioners and managers. This bibliography is subdivided into 40 categories including campaigns; consumer relations; community relations; corporate social responsibility;…

  11. Bibliography of Citizenship Materials

    Science.gov (United States)

    CASAS - Comprehensive Adult Student Assessment Systems (NJ1), 2008

    2008-01-01

    The 2008 CASAS "Bibliography of Citizenship Materials" lists available instructional resources for citizenship education. It focuses on materials appropriate for preparing people for the naturalization process and the standardized citizenship examination. Resources include textbooks, audio materials, software and Videos/DVDs. The bibliography also…

  12. Annotated Bibliography: Denmark

    DEFF Research Database (Denmark)

    Rytter, Jens Elo; Gøtze, Michael

    2008-01-01

    The bibliography includes the most important DaNISH BOOKS OF GENERAL INTEREST IN THE FIELD OF PUBLIC LAW IN THE PERIOD FROM 2006 - 2007.......The bibliography includes the most important DaNISH BOOKS OF GENERAL INTEREST IN THE FIELD OF PUBLIC LAW IN THE PERIOD FROM 2006 - 2007....

  13. Annual Bibliography, 2011

    Science.gov (United States)

    Brown, Jo. B., Comp.

    2012-01-01

    This classified, comprehensive bibliography for Appalachian studies includes books, journal articles, government documents, and selected newspaper articles published in 2010, plus relevant titles indexed in ProQuest Dissertations & Theses Database. Also, a number of earlier citations not included in the previous bibliography are listed here.…

  14. Bibliography of the Megapodiidae

    NARCIS (Netherlands)

    Dekker, R.W.R.J.

    1999-01-01

    The Proceedings of the First International Megapode Symposium (Zoologische Verhandelingen Leiden, vol. 278) published in December 1992 included the paper Bibliography of the Megapodiidae with references to literature on megapodes upto 1992 (Dekker & Jones, 1992: 57-78). The bibliography presented he

  15. LNG annotated bibliography

    Energy Technology Data Exchange (ETDEWEB)

    Bomelburg, H.J.; Counts, C.A.; Cowan, C.E.; Davis, W.E.; DeSteese, J.G.; Pelto, P.J.

    1982-09-01

    This document updates the bibliography published in Liquefied Gaseous Fuels Safety and Environmental Control Assessment Program: third status report (PNL-4172) and is a complete listing of literature reviewed and reported under the LNG Technical Surveillance Task. The bibliography is organized alphabetically by author.

  16. Alaska geothermal bibliography

    Energy Technology Data Exchange (ETDEWEB)

    Liss, S.A.; Motyka, R.J.; Nye, C.J. (comps.)

    1987-05-01

    The Alaska geothermal bibliography lists all publications, through 1986, that discuss any facet of geothermal energy in Alaska. In addition, selected publications about geology, geophysics, hydrology, volcanology, etc., which discuss areas where geothermal resources are located are included, though the geothermal resource itself may not be mentioned. The bibliography contains 748 entries.

  17. Art Therapy: A Bibliography.

    Science.gov (United States)

    Gantt, Linda, Comp.; Schmal, Marilyn Strauss, Comp.

    The bibliography on art therapy presents 1175 citations (1940-1973) drawn from searches of the medical indexes, computer systems of the National Library of Medicine and the National Institute of Mental Health, other bibliographies, Centre International de Documentation Concernant les Expressions Plastiques, and the American Journal of Art Therapy.…

  18. Annual Bibliography, 2011

    Science.gov (United States)

    Brown, Jo. B., Comp.

    2012-01-01

    This classified, comprehensive bibliography for Appalachian studies includes books, journal articles, government documents, and selected newspaper articles published in 2010, plus relevant titles indexed in ProQuest Dissertations & Theses Database. Also, a number of earlier citations not included in the previous bibliography are listed here.…

  19. Patient safety - the role of human factors and systems engineering.

    Science.gov (United States)

    Carayon, Pascale; Wood, Kenneth E

    2010-01-01

    Patient safety is a global challenge that requires knowledge and skills in multiple areas, including human factors and systems engineering. In this chapter, numerous conceptual approaches and methods for analyzing, preventing and mitigating medical errors are described. Given the complexity of healthcare work systems and processes, we emphasize the need for increasing partnerships between the health sciences and human factors and systems engineering to improve patient safety. Those partnerships will be able to develop and implement the system redesigns that are necessary to improve healthcare work systems and processes for patient safety.

  20. Use of Computers in Human Factors Engineering

    Science.gov (United States)

    1974-11-01

    SENSES (PHYSIOLOGY), THERMOPLASTIC RESINS, VISUAL ACUITY (U)R RESEARCH CONCERNS DETERMINATION OF THE INFORMATION PRESENTATION REQUIREMENTS OF HUMAN DATA...THE GEOMETRY OF THE wORK STATION, IS CURRENTLY BEING DEVELOPED. IT IS CALLED COMBIMAN, AN ACRONYM FOR COMPUTERIZED BIOMECHANICAL MAN- MODELo COMBIMAN

  1. Bibliography for Verification and Validation in Computational Simulations

    Energy Technology Data Exchange (ETDEWEB)

    Oberkampf, W.L.

    1998-10-01

    A bibliography has been compiled dealing with the verification and validation of computational simulations. The references listed in this bibliography are concentrated in the field of computational fluid dynamics (CFD). However, references from the following fields are also included: operations research, heat transfer, solid dynamics, software quality assurance, software accreditation, military systems, and nuclear reactor safety. This bibliography, containing 221 references, is not meant to be comprehensive. It was compiled during the last ten years in response to the author's interest and research in the methodology for verification and validation. The emphasis in the bibliography is in the following areas: philosophy of science underpinnings, development of terminology and methodology, high accuracy solutions for CFD verification, experimental datasets for CFD validation, and the statistical quantification of model validation. This bibliography should provide a starting point for individual researchers in many fields of computational simulation in science and engineering.

  2. Engineering large animal models of human disease.

    Science.gov (United States)

    Whitelaw, C Bruce A; Sheets, Timothy P; Lillico, Simon G; Telugu, Bhanu P

    2016-01-01

    The recent development of gene editing tools and methodology for use in livestock enables the production of new animal disease models. These tools facilitate site-specific mutation of the genome, allowing animals carrying known human disease mutations to be produced. In this review, we describe the various gene editing tools and how they can be used for a range of large animal models of diseases. This genomic technology is in its infancy but the expectation is that through the use of gene editing tools we will see a dramatic increase in animal model resources available for both the study of human disease and the translation of this knowledge into the clinic. Comparative pathology will be central to the productive use of these animal models and the successful translation of new therapeutic strategies.

  3. Genetically engineered mouse models and human osteosarcoma

    Directory of Open Access Journals (Sweden)

    Ng Alvin JM

    2012-10-01

    Full Text Available Abstract Osteosarcoma is the most common form of bone cancer. Pivotal insight into the genes involved in human osteosarcoma has been provided by the study of rare familial cancer predisposition syndromes. Three kindreds stand out as predisposing to the development of osteosarcoma: Li-Fraumeni syndrome, familial retinoblastoma and RecQ helicase disorders, which include Rothmund-Thomson Syndrome in particular. These disorders have highlighted the important roles of P53 and RB respectively, in the development of osteosarcoma. The association of OS with RECQL4 mutations is apparent but the relevance of this to OS is uncertain as mutations in RECQL4 are not found in sporadic OS. Application of the knowledge or mutations of P53 and RB in familial and sporadic OS has enabled the development of tractable, highly penetrant murine models of OS. These models share many of the cardinal features associated with human osteosarcoma including, importantly, a high incidence of spontaneous metastasis. The recent development of these models has been a significant advance for efforts to improve our understanding of the genetics of human OS and, more critically, to provide a high-throughput genetically modifiable platform for preclinical evaluation of new therapeutics.

  4. Human Factors Engineering and School Furniture: A Circular Odyssey.

    Science.gov (United States)

    Lane, Kenneth E.; Richardson, Michael D.

    1993-01-01

    A search reveals only six articles that concern human-factors engineering as it relates to student furniture. Contacts with five school-furniture manufacturers disclose that designs were basically unaltered for years and are claimed to reflect what schools want in furniture. Proposes recommendations to design and secure furniture to meet students'…

  5. Human Systems Engineering: A Leadership Model for Collaboration and Change.

    Science.gov (United States)

    Clark, Karen L.

    Human systems engineering (HSE) was created to introduce a new way of viewing collaboration. HSE emphasizes the role of leaders who welcome risk, commit to achieving positive change, and help others achieve change. The principles of HSE and its successful application to the collaborative process were illustrated through a case study representing a…

  6. Training human mesenchymal stromal cells for bone tissue engineering applications

    NARCIS (Netherlands)

    Doorn, J.

    2012-01-01

    Human mesenchymal stromal cells (hMSCs) are an interesting source for cell therapies and tissue engineering applications, because these cells are able to differentiate into various target tissues, such as bone, cartilage, fat and endothelial cells. In addition, they secrete a wide array of growth fa

  7. Bibliography: Current world literature.

    Science.gov (United States)

    2009-07-01

    This bibliography is compiled by clinicians from the journals listed at the end of this publication. It is based on literature entered into our database between 1 March 2008 and 28 February 2009 (articles are generally added to the database about two and a half months after publication). In addition, the bibliography contains every paper annotated by reviewers; these references were obtained from a variety of bibliographic databases and published between the beginning of the review period and the time of going to press. The bibliography has been grouped into topics that relate to the reviews in this issue.

  8. Bibliography. Current world literature.

    Science.gov (United States)

    2009-07-01

    This bibliography is compiled by clinicians from the journals listed at the end of this publication. It is based on literature entered into our database between 1 March 2008 and 28 February 2009 (articles are generally added to the database about two and a half months after publication). In addition, the bibliography contains every paper annotated by reviewers; these references were obtained from a variety of bibliographic databases and published between the beginning of the review period and the time of going to press. The bibliography has been grouped into topics that relate to the reviews in this issue.

  9. Human Factors in Nuclear Power Engineering in Polish Conditions

    Directory of Open Access Journals (Sweden)

    Agnieszka Kaczmarek-Kacprzak

    2014-09-01

    Full Text Available The paper “Human factors in nuclear power engineering in Polish conditions” focuses on analysis of dynamics of preparing Polish society to build fi rst nuclear power plant in XXI century in Poland. Authors compare experience from constructing nuclear power plant Sizewell B (Great Britain and Sizewell C, which is in preparation phase with polish nuclear power program. Paper includes aspects e.g. of creating nuclear safety culture and social opinion about investment. Human factors in nuclear power engineering are as well important as relevant economical and technical factors, but very often negligible. In Poland where history about Czarnobyl is still alive, and social opinion is created on emotions after accident in Fukushima, human factors are crucial and should be under comprehensive consideration.

  10. HOW DO RADIOLOGISTS USE THE HUMAN SEARCH ENGINE?

    Science.gov (United States)

    Wolfe, Jeremy M; Evans, Karla K; Drew, Trafton; Aizenman, Avigael; Josephs, Emilie

    2016-06-01

    Radiologists perform many 'visual search tasks' in which they look for one or more instances of one or more types of target item in a medical image (e.g. cancer screening). To understand and improve how radiologists do such tasks, it must be understood how the human 'search engine' works. This article briefly reviews some of the relevant work into this aspect of medical image perception. Questions include how attention and the eyes are guided in radiologic search? How is global (image-wide) information used in search? How might properties of human vision and human cognition lead to errors in radiologic search?

  11. Incremental Scheduling Engines for Human Exploration of the Cosmos

    Science.gov (United States)

    Jaap, John; Phillips, Shaun

    2005-01-01

    As humankind embarks on longer space missions farther from home, the requirements and environments for scheduling the activities performed on these missions are changing. As we begin to prepare for these missions it is appropriate to evaluate the merits and applicability of the different types of scheduling engines. Scheduling engines temporally arrange tasks onto a timeline so that all constraints and objectives are met and resources are not overbooked. Scheduling engines used to schedule space missions fall into three general categories: batch, mixed-initiative, and incremental. This paper presents an assessment of the engine types, a discussion of the impact of human exploration of the moon and Mars on planning and scheduling, and the applicability of the different types of scheduling engines. This paper will pursue the hypothesis that incremental scheduling engines may have a place in the new environment; they have the potential to reduce cost, to improve the satisfaction of those who execute or benefit from a particular timeline (the customers), and to allow astronauts to plan their own tasks and those of their companion robots.

  12. Integration of Social Sciences and Humanities into Mechanical Engineering Curriculum

    Directory of Open Access Journals (Sweden)

    Nikša Dubreta

    2014-04-01

    Full Text Available Article deals with ways in which social sciences and humanities have been integrated from the 1980s to the present day into curriculum of Faculty of Mechanical Engineering and Naval Architecture at University of Zagreb, Croatia. After a brief review and summary of selected research and theoretical contributions to the subject theme, a specific research setting is indicated and contextualized. Elements of socio-historical approach are established primarily through analysis of corresponding documents: curriculums from the 1980s, 1990s and 2000s and from key documents on strategic development of the Faculty. It is stressed that social sciences and humanities topics are continually represented in mechanical engineering study program as legitimate, but separate unit, poorly integrated in the main engineering courses. Together with more or less expressed orientation toward micro-social and micro-economical issues in industry and business, it points to the main features in continuity of establishing the field of social sciences and humanities. Finally, it is shown that chances to widen and enrich aforementioned field are in close relation to the character of engineering and its social contextualization expressed in a key Faculty’s strategic documents.

  13. Product Modelling for Building Design: Annotated Bibliography (2nd Edition)

    DEFF Research Database (Denmark)

    Galle, Per

    1999-01-01

    This bibliography concerns research publications from 1976 to 1994-5, on product modelling in computer aided architectural design and computer aided engineering design of buildings and their surroundings. For each item of literature, full bibliographic information is given whenever available...... of literature is offered on machine interpretation of drawings, which may be relevant in the context of information exchange among different product models. Although the bibliography is fairly comprehensive as far as it goes, no completeness of coverage is claimed....

  14. Product Modelling for Building Design: Annotated Bibliography (2nd Edition)

    DEFF Research Database (Denmark)

    Galle, Per

    1999-01-01

    This bibliography concerns research publications from 1976 to 1994-5, on product modelling in computer aided architectural design and computer aided engineering design of buildings and their surroundings. For each item of literature, full bibliographic information is given whenever available...... of literature is offered on machine interpretation of drawings, which may be relevant in the context of information exchange among different product models. Although the bibliography is fairly comprehensive as far as it goes, no completeness of coverage is claimed....

  15. Bibliography on Alaska estuaries

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This bibliography was compiled to assist in working up “profiles” for the estuaries in Alaska. The purpose of the profiles is to list in a narrative form the...

  16. Bibliography of Fynbos ecology

    CSIR Research Space (South Africa)

    Jarman, ML

    1981-08-01

    Full Text Available References to 814 publications are presented, together with keyword listings and a keyword index. This bibliography forms part of the Phase I review and synthesis activity of the Fynbos Biome Project....

  17. Bibliographies. The Librarian's Bookshelf.

    Science.gov (United States)

    Bourdon, Cathleen

    2002-01-01

    This bibliography for materials helpful to librarians includes works relating to administration and personnel; bibliographic instruction; information literacy; cataloging and classification; children's and adolescent services and materials; collection development; copyright; distance education; electronic libraries; evaluation; fund raising;…

  18. Bibliographies: The Librarian's Bookshelf.

    Science.gov (United States)

    Bourdon, Cathleen

    2003-01-01

    This selective bibliography is organized by subject headings that include administration and personnel; bibliographic instruction/information literacy; cataloging and classification; children's and young adults; collection development; copyright; customer service; distance education; electronic libraries; evaluation of library services; fund…

  19. 2014 Space Human Factors Engineering Standing Review Panel

    Science.gov (United States)

    Steinberg, Susan

    2014-01-01

    The 2014 Space Human Factors Engineering (SHFE) Standing Review Panel (from here on referred to as the SRP) participated in a WebEx/teleconference with members of the Space Human Factors and Habitability (SHFH) Element, representatives from the Human Research Program (HRP), the National Space Biomedical Research Institute (NSBRI), and NASA Headquarters on November 17, 2014 (list of participants is in Section XI of this report). The SRP reviewed the updated research plans for the Risk of Incompatible Vehicle/Habitat Design (HAB Risk) and the Risk of Performance Errors Due to Training Deficiencies (Train Risk). The SRP also received a status update on the Risk of Inadequate Critical Task Design (Task Risk), the Risk of Inadequate Design of Human and Automation/Robotic Integration (HARI Risk), and the Risk of Inadequate Human-Computer Interaction (HCI Risk).

  20. GSV Annotated Bibliography

    Energy Technology Data Exchange (ETDEWEB)

    Roberts, Randy S. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Pope, Paul A. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Jiang, Ming [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Trucano, Timothy G. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Aragon, Cecilia R. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Ni, Kevin [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Wei, Thomas [Argonne National Lab. (ANL), Argonne, IL (United States); Chilton, Lawrence K. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Bakel, Alan [Argonne National Lab. (ANL), Argonne, IL (United States)

    2011-06-14

    The following annotated bibliography was developed as part of the Geospatial Algorithm Veri cation and Validation (GSV) project for the Simulation, Algorithms and Modeling program of NA-22. Veri cation and Validation of geospatial image analysis algorithms covers a wide range of technologies. Papers in the bibliography are thus organized into the following ve topic areas: Image processing and analysis, usability and validation of geospatial image analysis algorithms, image distance measures, scene modeling and image rendering, and transportation simulation models.

  1. Decentralized energy studies: bibliography

    Energy Technology Data Exchange (ETDEWEB)

    Ohi, J.M.; Unseld, C.T.; Levine, A.; Silversmith, J.A.

    1980-05-01

    This bibliography is a compilation of literature on decentralized energy systems. It is arranged according to topical (e.g., lifestyle and values, institutions, and economics) and geographical scale to facilitate quick reference to specific areas of interest. Also included are articles by and about Amory B. Lovins who has played a pivotal role in making energy decentralization an important topic of national debate. Periodicals, other bibliographies, and directories are also listed.

  2. Site-Specific Genome Engineering in Human Pluripotent Stem Cells

    Directory of Open Access Journals (Sweden)

    Sylvia Merkert

    2016-06-01

    Full Text Available The possibility to generate patient-specific induced pluripotent stem cells (iPSCs offers an unprecedented potential of applications in clinical therapy and medical research. Human iPSCs and their differentiated derivatives are tools for diseases modelling, drug discovery, safety pharmacology, and toxicology. Moreover, they allow for the engineering of bioartificial tissue and are promising candidates for cellular therapies. For many of these applications, the ability to genetically modify pluripotent stem cells (PSCs is indispensable, but efficient site-specific and safe technologies for genetic engineering of PSCs were developed only recently. By now, customized engineered nucleases provide excellent tools for targeted genome editing, opening new perspectives for biomedical research and cellular therapies.

  3. Site-Specific Genome Engineering in Human Pluripotent Stem Cells.

    Science.gov (United States)

    Merkert, Sylvia; Martin, Ulrich

    2016-06-24

    The possibility to generate patient-specific induced pluripotent stem cells (iPSCs) offers an unprecedented potential of applications in clinical therapy and medical research. Human iPSCs and their differentiated derivatives are tools for diseases modelling, drug discovery, safety pharmacology, and toxicology. Moreover, they allow for the engineering of bioartificial tissue and are promising candidates for cellular therapies. For many of these applications, the ability to genetically modify pluripotent stem cells (PSCs) is indispensable, but efficient site-specific and safe technologies for genetic engineering of PSCs were developed only recently. By now, customized engineered nucleases provide excellent tools for targeted genome editing, opening new perspectives for biomedical research and cellular therapies.

  4. Human factors engineering report for the cold vacuum drying facility

    Energy Technology Data Exchange (ETDEWEB)

    IMKER, F.W.

    1999-06-30

    The purpose of this report is to present the results and findings of the final Human Factors Engineering (HFE) technical analysis and evaluation of the Cold Vacuum Drying Facility (CVDF). Ergonomics issues are also addressed in this report, as appropriate. This report follows up and completes the preliminary work accomplished and reported by the Preliminary HFE Analysis report (SNF-2825, Spent Nuclear Fuel Project Cold Vacuum Drying Facility Human Factors Engineering Analysis: Results and Findings). This analysis avoids redundancy of effort except for ensuring that previously recommended HFE design changes have not affected other parts of the system. Changes in one part of the system may affect other parts of the system where those changes were not applied. The final HFE analysis and evaluation of the CVDF human-machine interactions (HMI) was expanded to include: the physical work environment, human-computer interface (HCI) including workstation and software, operator tasks, tools, maintainability, communications, staffing, training, and the overall ability of humans to accomplish their responsibilities, as appropriate. Key focal areas for this report are the process bay operations, process water conditioning (PWC) skid, tank room, and Central Control Room operations. These key areas contain the system safety-class components and are the foundation for the human factors design basis of the CVDF.

  5. DRUMS: a human disease related unique gene mutation search engine.

    Science.gov (United States)

    Li, Zuofeng; Liu, Xingnan; Wen, Jingran; Xu, Ye; Zhao, Xin; Li, Xuan; Liu, Lei; Zhang, Xiaoyan

    2011-10-01

    With the completion of the human genome project and the development of new methods for gene variant detection, the integration of mutation data and its phenotypic consequences has become more important than ever. Among all available resources, locus-specific databases (LSDBs) curate one or more specific genes' mutation data along with high-quality phenotypes. Although some genotype-phenotype data from LSDB have been integrated into central databases little effort has been made to integrate all these data by a search engine approach. In this work, we have developed disease related unique gene mutation search engine (DRUMS), a search engine for human disease related unique gene mutation as a convenient tool for biologists or physicians to retrieve gene variant and related phenotype information. Gene variant and phenotype information were stored in a gene-centred relational database. Moreover, the relationships between mutations and diseases were indexed by the uniform resource identifier from LSDB, or another central database. By querying DRUMS, users can access the most popular mutation databases under one interface. DRUMS could be treated as a domain specific search engine. By using web crawling, indexing, and searching technologies, it provides a competitively efficient interface for searching and retrieving mutation data and their relationships to diseases. The present system is freely accessible at http://www.scbit.org/glif/new/drums/index.html.

  6. Cooperative and Human Aspects of Software Engineering (CHASE 2010)

    DEFF Research Database (Denmark)

    Dittrich, Yvonne; De Souza, Cleidson; Korpela, Mikko

    2010-01-01

    Software is created by people---software engineers---working in varied environments, under various conditions. Thus understanding cooperative and human aspect of software development is crucial to comprehend how methods and tools are used, and thereby improving the creation and maintenance...... research on human and cooperative aspects of software engineering. We aim at providing both a meeting place for the growing community and the possibility for researchers interested in joining the field to present their work in progress and get an overview over the field....... of software. Inspired by the hosting country's concept of co-responsibility -- ubuntu -- we especially invited contributions that address community-based development like open source development and sustainability of ICT eco-systems. The goal of this workshop is to provide a forum for discussing high quality...

  7. Cooperative and Human Aspects of Software Engineering (CHASE 2010)

    DEFF Research Database (Denmark)

    Dittrich, Yvonne; De Souza, Cleidson; Korpela, Mikko

    2010-01-01

    of software. Inspired by the hosting country's concept of co-responsibility -- ubuntu -- we especially invited contributions that address community-based development like open source development and sustainability of ICT eco-systems. The goal of this workshop is to provide a forum for discussing high quality......Software is created by people---software engineers---working in varied environments, under various conditions. Thus understanding cooperative and human aspect of software development is crucial to comprehend how methods and tools are used, and thereby improving the creation and maintenance...... research on human and cooperative aspects of software engineering. We aim at providing both a meeting place for the growing community and the possibility for researchers interested in joining the field to present their work in progress and get an overview over the field....

  8. Computer aided systems human engineering: A hypermedia tool

    Science.gov (United States)

    Boff, Kenneth R.; Monk, Donald L.; Cody, William J.

    1992-01-01

    The Computer Aided Systems Human Engineering (CASHE) system, Version 1.0, is a multimedia ergonomics database on CD-ROM for the Apple Macintosh II computer, being developed for use by human system designers, educators, and researchers. It will initially be available on CD-ROM and will allow users to access ergonomics data and models stored electronically as text, graphics, and audio. The CASHE CD-ROM, Version 1.0 will contain the Boff and Lincoln (1988) Engineering Data Compendium, MIL-STD-1472D and a unique, interactive simulation capability, the Perception and Performance Prototyper. Its features also include a specialized data retrieval, scaling, and analysis capability and the state of the art in information retrieval, browsing, and navigation.

  9. Concise review: Human cell engineering: cellular reprogramming and genome editing.

    Science.gov (United States)

    Mali, Prashant; Cheng, Linzhao

    2012-01-01

    Cell engineering is defined here as the collective ability to both reset and edit the genome of a mammalian cell. Until recently, this had been extremely challenging to achieve as nontransformed human cells are significantly refractory to both these processes. The recent success in reprogramming somatic cells into induced pluripotent stem cells that are self-renewable in culture, coupled with our increasing ability to effect precise and predesigned genomic editing, now readily permits cellular changes at both the genetic and epigenetic levels. These dual capabilities also make possible the generation of genetically matched, disease-free stem cells from patients for regenerative medicine. The objective of this review is to summarize the key enabling developments on these two rapidly evolving research fronts in human cell engineering, highlight unresolved issues, and outline potential future research directions.

  10. Tissue engineered humanized bone supports human hematopoiesis in vivo.

    Science.gov (United States)

    Holzapfel, Boris M; Hutmacher, Dietmar W; Nowlan, Bianca; Barbier, Valerie; Thibaudeau, Laure; Theodoropoulos, Christina; Hooper, John D; Loessner, Daniela; Clements, Judith A; Russell, Pamela J; Pettit, Allison R; Winkler, Ingrid G; Levesque, Jean-Pierre

    2015-08-01

    Advances in tissue-engineering have resulted in a versatile tool-box to specifically design a tailored microenvironment for hematopoietic stem cells (HSCs) in order to study diseases that develop within this setting. However, most current in vivo models fail to recapitulate the biological processes seen in humans. Here we describe a highly reproducible method to engineer humanized bone constructs that are able to recapitulate the morphological features and biological functions of the HSC niches. Ectopic implantation of biodegradable composite scaffolds cultured for 4 weeks with human mesenchymal progenitor cells and loaded with rhBMP-7 resulted in the development of a chimeric bone organ including a large number of human mesenchymal cells which were shown to be metabolically active and capable of establishing a humanized microenvironment supportive of the homing and maintenance of human HSCs. A syngeneic mouse-to-mouse transplantation assay was used to prove the functionality of the tissue-engineered ossicles. We predict that the ability to tissue engineer a morphologically intact and functional large-volume bone organ with a humanized bone marrow compartment will help to further elucidate physiological or pathological interactions between human HSCs and their native niches. Crown Copyright © 2015. Published by Elsevier Ltd. All rights reserved.

  11. Engineering Education Development to Enhance Human Skill in DENSO

    Science.gov (United States)

    Isogai, Emiko; Nuka, Takeji

    Importance of human skills such as communication or instruction capability to their staff members has recently been highlighted in a workplace, due to decreasing opportunity of face-to-face communication between supervisors and their staff, or Instruction capability through OJT (On the Job Training) . Currently, communication skills are being reinforced mainly through OJT at DENSO. Therefore, as part of supplemental support tools, DENSO has established comprehensive engineers training program on off-JT basis for developing human skills, covering from newly employeed enginners up to managerial class since 2003. This paper describes education activities and reports the results.

  12. TEPCO's Approach to Power-Engineer Human Resource Development

    Science.gov (United States)

    Sato, Masaki

    We think 'human resources and technology' is developed only by self-training continuously, keeping higher motivation and practicing repeatedly. Moreover it is indispensable for sustainable development of company. Management vision, top-down message with vertical communication, and bottom-up systematic approaches are necessary for sustainable human resource development, sharing the value with coordination, and in addition, OJT and Off-JT method should be used effectively. This paper shows TEPCO's attempts to develop engineers' technical skills as a reference of a in-company continuing professional development.

  13. Tissue-engineered models of human tumors for cancer research

    Science.gov (United States)

    Villasante, Aranzazu; Vunjak-Novakovic, Gordana

    2015-01-01

    Introduction Drug toxicity often goes undetected until clinical trials, which are the most costly and dangerous phase of drug development. Both the cultures of human cells and animal studies have limitations that cannot be overcome by incremental improvements in drug-testing protocols. A new generation of bioengineered tumors is now emerging in response to these limitations, with potential to transform drug screening by providing predictive models of tumors within their tissue context, for studies of drug safety and efficacy. An area that could greatly benefit from these models is cancer research. Areas covered In this review, the authors first describe the engineered tumor systems, using Ewing's sarcoma as an example of human tumor that cannot be predictably studied in cell culture and animal models. Then, they discuss the importance of the tissue context for cancer progression and outline the biomimetic principles for engineering human tumors. Finally, they discuss the utility of bioengineered tumor models for cancer research and address the challenges in modeling human tumors for use in drug discovery and testing. Expert opinion While tissue models are just emerging as a new tool for cancer drug discovery, they are already demonstrating potential for recapitulating, in vitro, the native behavior of human tumors. Still, numerous challenges need to be addressed before we can have platforms with a predictive power appropriate for the pharmaceutical industry. Some of the key needs include the incorporation of the vascular compartment, immune system components, and mechanical signals that regulate tumor development and function. PMID:25662589

  14. Human factors engineering plan for reviewing nuclear plant modernization programs

    Energy Technology Data Exchange (ETDEWEB)

    O' Hara, John; Higgins, James [Brookhaven National Laboratory, Upton, NY (United States)

    2004-12-01

    The Swedish Nuclear Power Inspectorate reviews the human factors engineering (HFE) aspects of nuclear power plants (NPPs) involved in the modernization of the plant systems and control rooms. The purpose of a HFE review is to help ensure personnel and public safety by verifying that accepted HFE practices and guidelines are incorporated into the program and nuclear power plant design. Such a review helps to ensure the HFE aspects of an NPP are developed, designed, and evaluated on the basis of a structured top-down system analysis using accepted HFE principles. The review addresses eleven HFE elements: HFE Program Management, Operating Experience Review, Functional Requirements Analysis and Allocation, Task Analysis, Staffing, Human Reliability Analysis, Human-System Interface Design, Procedure Development, Training Program Development, Human Factors Verification and Validation, and Design Implementation.

  15. Bibliographie Annotee de Linguistique Acadienne (Annotated Bibliography of Acadian Linguistics).

    Science.gov (United States)

    Gesner, Edward

    A bibliography of 430 books, journal articles, papers, and other references on Acadian French written in English or French is divided into two principal sections: an annotated bibliography of works focusing on the Acadian French dialect spoken in the Canadian Maritime Provinces, and an unannotated bibliography pertaining to Louisiana Acadian…

  16. Production tests 300 series: A Bibliography

    Energy Technology Data Exchange (ETDEWEB)

    Schmidt, B.J.

    1957-03-01

    This bibliography is a compilation of reports on production tests performed in the 300 Area from May 1944 to February 1957. It includes all tests covering engineering and physics experiments in the Hanford Test Reactor development; fabrication and subsequent irradiation of fuel elements and melt plant (slug fabrication) procedures. Subject, author and report number indexes are included. Addendum 1 gives the reactor production test number under which the fuel elements were irradiated.

  17. Human factors engineering checklists for application in the SAR process

    Energy Technology Data Exchange (ETDEWEB)

    Overlin, T.K.; Romero, H.A.; Ryan, T.G.

    1995-03-01

    This technical report was produced to assist the preparers and reviewers of the human factors portions of the SAR in completing their assigned tasks regarding analysis and/or review of completed analyses. The checklists, which are the main body of the report, and the subsequent tables, were developed to assist analysts in generating the needed analysis data to complete the human engineering analysis for the SAR. The technical report provides a series of 19 human factors engineering (HFE) checklists which support the safety analyses of the US Department of Energy`s (DOE) reactor and nonreactor facilities and activities. The results generated using these checklists and in the preparation of the concluding analyses provide the technical basis for preparing the human factors chapter, and subsequent inputs to other chapters, required by DOE as a part of the safety analysis reports (SARs). This document is divided into four main sections. The first part explains the origin of the checklists, the sources utilized, and other information pertaining to the purpose and scope of the report. The second part, subdivided into 19 sections, is the checklists themselves. The third section is the glossary which defines terms that could either be unfamiliar or have specific meanings within the context of these checklists. The final section is the subject index in which the glossary terms are referenced back to the specific checklist and page the term is encountered.

  18. Mechanical stimulation improves tissue-engineered human skeletal muscle

    Science.gov (United States)

    Powell, Courtney A.; Smiley, Beth L.; Mills, John; Vandenburgh, Herman H.

    2002-01-01

    Human bioartificial muscles (HBAMs) are tissue engineered by suspending muscle cells in collagen/MATRIGEL, casting in a silicone mold containing end attachment sites, and allowing the cells to differentiate for 8 to 16 days. The resulting HBAMs are representative of skeletal muscle in that they contain parallel arrays of postmitotic myofibers; however, they differ in many other morphological characteristics. To engineer improved HBAMs, i.e., more in vivo-like, we developed Mechanical Cell Stimulator (MCS) hardware to apply in vivo-like forces directly to the engineered tissue. A sensitive force transducer attached to the HBAM measured real-time, internally generated, as well as externally applied, forces. The muscle cells generated increasing internal forces during formation which were inhibitable with a cytoskeleton depolymerizer. Repetitive stretch/relaxation for 8 days increased the HBAM elasticity two- to threefold, mean myofiber diameter 12%, and myofiber area percent 40%. This system allows engineering of improved skeletal muscle analogs as well as a nondestructive method to determine passive force and viscoelastic properties of the resulting tissue.

  19. Mechanical stimulation improves tissue-engineered human skeletal muscle

    Science.gov (United States)

    Powell, Courtney A.; Smiley, Beth L.; Mills, John; Vandenburgh, Herman H.

    2002-01-01

    Human bioartificial muscles (HBAMs) are tissue engineered by suspending muscle cells in collagen/MATRIGEL, casting in a silicone mold containing end attachment sites, and allowing the cells to differentiate for 8 to 16 days. The resulting HBAMs are representative of skeletal muscle in that they contain parallel arrays of postmitotic myofibers; however, they differ in many other morphological characteristics. To engineer improved HBAMs, i.e., more in vivo-like, we developed Mechanical Cell Stimulator (MCS) hardware to apply in vivo-like forces directly to the engineered tissue. A sensitive force transducer attached to the HBAM measured real-time, internally generated, as well as externally applied, forces. The muscle cells generated increasing internal forces during formation which were inhibitable with a cytoskeleton depolymerizer. Repetitive stretch/relaxation for 8 days increased the HBAM elasticity two- to threefold, mean myofiber diameter 12%, and myofiber area percent 40%. This system allows engineering of improved skeletal muscle analogs as well as a nondestructive method to determine passive force and viscoelastic properties of the resulting tissue.

  20. Engineering melanoma progression in a humanized environment in vivo.

    Science.gov (United States)

    Kiowski, Gregor; Biedermann, Thomas; Widmer, Daniel S; Civenni, Gianluca; Burger, Charlotte; Dummer, Reinhard; Sommer, Lukas; Reichmann, Ernst

    2012-01-01

    To overcome the lack of effective therapeutics for aggressive melanoma, new research models closely resembling the human disease are required. Here we report the development of a fully orthotopic, humanized in vivo model for melanoma, faithfully recapitulating human disease initiation and progression. To this end, human melanoma cells were seeded into engineered human dermo-epidermal skin substitutes. Transplantation onto the back of immunocompromised rats consistently resulted in the development of melanoma, displaying the hallmarks of their parental tumors. Importantly, all initial steps of disease progression were recapitulated, including the incorporation of the tumor cells into their physiological microenvironment, transition of radial to vertical growth, and establishment of highly vascularized, aggressive tumors with dermal involvement. Because all cellular components can be individually accessed using this approach, it allows manipulation of the tumor cells, as well as of the keratinocyte and stromal cell populations. Therefore, in one defined model system, tumor cell-autonomous and non-autonomous pathways regulating human disease progression can be investigated in a humanized, clinically relevant context.

  1. Maturing human pluripotent stem cell-derived cardiomyocytes in human engineered cardiac tissues.

    Science.gov (United States)

    Feric, Nicole T; Radisic, Milica

    2016-01-15

    Engineering functional human cardiac tissue that mimics the native adult morphological and functional phenotype has been a long held objective. In the last 5 years, the field of cardiac tissue engineering has transitioned from cardiac tissues derived from various animal species to the production of the first generation of human engineered cardiac tissues (hECTs), due to recent advances in human stem cell biology. Despite this progress, the hECTs generated to date remain immature relative to the native adult myocardium. In this review, we focus on the maturation challenge in the context of hECTs, the present state of the art, and future perspectives in terms of regenerative medicine, drug discovery, preclinical safety testing and pathophysiological studies. Copyright © 2015 Elsevier B.V. All rights reserved.

  2. Large space structures and systems in the space station era: A bibliography with indexes (supplement 03)

    Science.gov (United States)

    1991-01-01

    Bibliographies and abstracts are listed for 1221 reports, articles, and other documents introduced into the NASA scientific and technical information system between January 1, 1991 and June 30, 1991. Topics covered include large space structures and systems, space stations, extravehicular activity, thermal environments and control, tethering, spacecraft power supplies, structural concepts and control systems, electronics, advanced materials, propulsion, policies and international cooperation, vibration and dynamic controls, robotics and remote operations, data and communication systems, electric power generation, space commercialization, orbital transfer, and human factors engineering.

  3. TWRS privatization bibliography

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-03-01

    The purpose of this bibliography is to present a select set of documents that may be of interest to the Offeror, covering a variety of subject areas related to the TWRS Privatization Project. This bibliography is not, nor is intended to be, exhaustive or complete. It was prepared with the intent of providing a sampling of representative documents potentially helpful to Offerors. The documents referenced herein have been identified as representative of those potentially helpful to Offerors. This list of documents does not represent the full extent of available and potentially helpful information, nor should it be taken as a representation of documents determined to be of greater importance than other documents not referenced herein. There are numerous documents available to the public that are NOT cited in this bibliography; the Offeror is encouraged to perform searches for alternate sources of information.

  4. Bibliography of Supersonic Cruise Research (SCR) program from 1980 to 1983

    Science.gov (United States)

    Hoffman, S.

    1984-01-01

    A bibliography for the Supersonic Cruise Research (SCR) and Variable Cycle Engine (VCE) Programs is presented. An annotated bibliography for the last 123 formal reports and a listing of titles for 44 articles and presentations is included. The studies identifies technologies for producing efficient supersonic commercial jet transports for cruise Mach numbers from 2.0 to 2.7.

  5. Automatic differentiation bibliography

    Energy Technology Data Exchange (ETDEWEB)

    Corliss, G.F. (comp.)

    1992-07-01

    This is a bibliography of work related to automatic differentiation. Automatic differentiation is a technique for the fast, accurate propagation of derivative values using the chain rule. It is neither symbolic nor numeric. Automatic differentiation is a fundamental tool for scientific computation, with applications in optimization, nonlinear equations, nonlinear least squares approximation, stiff ordinary differential equation, partial differential equations, continuation methods, and sensitivity analysis. This report is an updated version of the bibliography which originally appeared in Automatic Differentiation of Algorithms: Theory, Implementation, and Application.

  6. The Miner, His Job and His Environment: A Review and Bibliography of Selected Recent Research on Human Performance.

    Science.gov (United States)

    Fried, Charles; And Others

    In a survey of the state of the art in ergonomics (human factors) as it has been applied to coal mining, approximately 1,200 references in the published literature both foreign and domestic were located. Abstracts of 49 references were prepared on such topics as the effects of environmental stress on health and productivity, accident prevention…

  7. `Human nature': Chemical engineering students' ideas about human relationships with the natural world

    Science.gov (United States)

    Goldman, Daphne; Ben-Zvi Assaraf, Orit; Shemesh, Julia

    2014-05-01

    While importance of environmental ethics, as a component of sustainable development, in preparing engineers is widely acknowledged, little research has addressed chemical engineers' environmental concerns. This study aimed to address this void by exploring chemical engineering students' values regarding human-nature relationships. The study was conducted with 247 3rd-4th year chemical engineering students in Israeli Universities. It employed the New Ecological Paradigm (NEP)-questionnaire to which students added written explanations. Quantitative analysis of NEP-scale results shows that the students demonstrated moderately ecocentric orientation. Explanations to the NEP-items reveal diverse, ambivalent ideas regarding the notions embodied in the NEP, strong scientific orientation and reliance on technology for addressing environmental challenges. Endorsing sustainability implies that today's engineers be equipped with an ecological perspective. The capacity of Higher Education to enable engineers to develop dispositions about human-nature interrelationships requires adaptation of curricula towards multidisciplinary, integrative learning addressing social-political-economic-ethical perspectives, and implementing critical-thinking within the socio-scientific issues pedagogical approach.

  8. Design Considerations for Human Rating of Liquid Rocket Engines

    Science.gov (United States)

    Parkinson, Douglas

    2010-01-01

    I.Human-rating is specific to each engine; a. Context of program/project must be understood. b. Engine cannot be discussed independently from vehicle and mission. II. Utilize a logical combination of design, manufacturing, and test approaches a. Design 1) It is crucial to know the potential ways a system can fail, and how a failure can propagate; 2) Fault avoidance, fault tolerance, DFMR, caution and warning all have roles to play. b. Manufacturing and Assembly; 1) As-built vs. as-designed; 2) Review procedures for assembly and maintenance periodically; and 3) Keep personnel trained and certified. c. There is no substitute for test: 1) Analytical tools are constantly advancing, but still need test data for anchoring assumptions; 2) Demonstrate robustness and explore sensitivities; 3) Ideally, flight will be encompassed by ground test experience. III. Consistency and repeatability is key in production a. Maintain robust processes and procedures for inspection and quality control based upon development and qualification experience; b. Establish methods to "spot check" quality and consistency in parts: 1) Dedicated ground test engines; 2) Random components pulled from the line/lot to go through "enhanced" testing.

  9. Human resources in science and engineering: Policy implications

    Energy Technology Data Exchange (ETDEWEB)

    Leggon, C.B.

    1995-12-31

    Recently, there has been much debate concerning the adequacy of the United States` (U.S.) human resources base to meet its future needs for science and engineering (S/E) talent. Science policy analysts - and scientists and engineers themselves - disagree about whether there will be any shortages of scientists and engineers, and if so, what they will mean for the U.S. Whether or not these shortages materialize, it is necessary for the U.S. to expand the pool from which it recruits its S/E talent. This paper addresses the question of how to increases the diversity of the S/E talent pool to include those who are projected by the year 2000 to be the majority of entry-level workers in the U.S. workforce: women and racial/ethnic minorities. Market forces alone cannot increase the size and diversity of the U.S. S/E workforce. Policy intervention will continue to be required to increase the diversity of the S/E workforce.

  10. Advancing biomaterials of human origin for tissue engineering.

    Science.gov (United States)

    Chen, Fa-Ming; Liu, Xiaohua

    2016-02-01

    Biomaterials have played an increasingly prominent role in the success of biomedical devices and in the development of tissue engineering, which seeks to unlock the regenerative potential innate to human tissues/organs in a state of deterioration and to restore or reestablish normal bodily function. Advances in our understanding of regenerative biomaterials and their roles in new tissue formation can potentially open a new frontier in the fast-growing field of regenerative medicine. Taking inspiration from the role and multi-component construction of native extracellular matrices (ECMs) for cell accommodation, the synthetic biomaterials produced today routinely incorporate biologically active components to define an artificial in vivo milieu with complex and dynamic interactions that foster and regulate stem cells, similar to the events occurring in a natural cellular microenvironment. The range and degree of biomaterial sophistication have also dramatically increased as more knowledge has accumulated through materials science, matrix biology and tissue engineering. However, achieving clinical translation and commercial success requires regenerative biomaterials to be not only efficacious and safe but also cost-effective and convenient for use and production. Utilizing biomaterials of human origin as building blocks for therapeutic purposes has provided a facilitated approach that closely mimics the critical aspects of natural tissue with regard to its physical and chemical properties for the orchestration of wound healing and tissue regeneration. In addition to directly using tissue transfers and transplants for repair, new applications of human-derived biomaterials are now focusing on the use of naturally occurring biomacromolecules, decellularized ECM scaffolds and autologous preparations rich in growth factors/non-expanded stem cells to either target acceleration/magnification of the body's own repair capacity or use nature's paradigms to create new tissues for

  11. Characterization of human myoblast cultures for tissue engineering.

    Science.gov (United States)

    Stern-Straeter, Jens; Bran, Gregor; Riedel, Frank; Sauter, Alexander; Hörmann, Karl; Goessler, Ulrich Reinhart

    2008-01-01

    Skeletal muscle tissue engineering, a promising specialty, aims at the reconstruction of skeletal muscle loss. In vitro tissue engineering attempts to achieve this goal by creating differentiated, functional muscle tissue through a process in which stem cells are extracted from the patient, e.g. by muscle biopsies, expanded and differentiated in a controlled environment, and subsequently re-implanted. A prerequisite for this undertaking is the ability to cultivate and differentiate human skeletal muscle cell cultures. Evidently, optimal culture conditions must be investigated for later clinical utilization. We therefore analysed the proliferation of human cells in different environments and evaluated the differentiation potential of different culture media. It was shown that human myoblasts have a higher rate of proliferation in the alamarBlue assay when cultured on gelatin-coated culture flasks rather than polystyrene-coated flasks. We also demonstrated that myoblasts treated with a culture medium with a high concentration of growth factors [growth medium (GM)] showed a higher proliferation compared to cultures treated with a culture medium with lower amounts of growth factors [differentiation medium (DM)]. Differentiation of human myoblast cell cultures treated with GM and DM was analysed until day 16 and myogenesis was verified by expression of MyoD, myogenin, alpha-sarcomeric actin and myosin heavy chain by semi-quantitative RT-PCR. Immunohistochemical staining for desmin, Myf-5 and alpha-sarcomeric actin was performed to verify the myogenic phenotype of extracted satellite cells and to prove the maturation of cells. Cultures treated with DM showed positive staining for alpha-sarcomeric actin. Notably, markers of differentiation were also detected in cultures treated with GM, but there was no formation of myotubes. In the enzymatic assay of creatine phosphokinase, cultures treated with DM showed a higher activity, evidencing a higher degree of differentiation

  12. Evaluation Utilization: A Bibliography.

    Science.gov (United States)

    King, Jean A.; And Others

    This bibliography cites 326 references on the use of evaluation information and results. References include dissertations, journal articles, ERIC documents, books, and other papers. Both published and unpublished works, the majority of which were written after 1970, are cited. A special list of ten recommended readings is separate from the…

  13. Cheating. An Annotated Bibliography.

    Science.gov (United States)

    Wildemuth, Barbara M., Comp.

    This 89-item, annotated bibliography was compiled to provide access to research and discussions of cheating and, specifically, cheating on tests. It is not limited to any educational level, nor is it confined to any specific curriculum area. Two data bases were searched by computer, and a library search was conducted. A computer search of the…

  14. A Bibliography of Virgil.

    Science.gov (United States)

    Austin, R. G., Comp.

    This bibliography contains works largely in English listed in 10 categories. They include: (1) books about Virgil or relevant to his poetry, (2) editions and commentaries, (3) translations, and (4) articles in the "Journal of Roman Studies," the "Classical Quarterly," the "Classical Review,""Greece and Rome," the "American Journal of…

  15. Parent Education: Abstract Bibliography.

    Science.gov (United States)

    Kremer, Barbara, Comp.

    This bibliography has been compiled to alert educators to parent education documents found in the ERIC microfiche collection and in journal literature. Abstracts of selected documents have been taken from "Research in Education (RIE)", and journal article citations from the "Current Index to Journals in Education (CIJE)". Included are published…

  16. Selected Resources and Bibliography

    Science.gov (United States)

    New Directions for Higher Education, 2011

    2011-01-01

    This chapter provides an annotated bibliography of resources pertaining to international branch campuses (IBCs). This collection of references has been selected to represent the breadth of emerging scholarship on cross-border higher education and is intended to provide further resources on a range of concerns surrounding cross-border higher…

  17. Food Stamps: A Bibliography.

    Science.gov (United States)

    Cameron, Colin; And Others

    A voluminous body of literature exists on the topic of food stamps including the administration of the federal and state programs and the question of abuses and fraud. This bibliography describes a portion of that material in print. An introduction provides general information and a chronology of the food stamp program and comments on the place of…

  18. Information Design: A Bibliography.

    Science.gov (United States)

    Albers, Michael J.; Lisberg, Beth Conney

    2000-01-01

    Presents a 17-item annotated list of essential books on information design chosen by members of the InfoDesign e-mail list. Includes a 113-item unannotated bibliography of additional works, on topics of creativity and critical thinking; visual thinking; graphic design; infographics; information design; instructional design; interface design;…

  19. Annotated Bibliography. First Edition.

    Science.gov (United States)

    Haring, Norris G.

    An annotated bibliography which presents approximately 300 references from 1951 to 1973 on the education of severely/profoundly handicapped persons. Citations are grouped alphabetically by author's name within the following categories: characteristics and treatment, gross motor development, sensory and motor development, physical therapy for the…

  20. Manpower Planning; A Bibliography.

    Science.gov (United States)

    Lewis, C. G., Ed.; And Others

    This bibliography is intended as a reference on manpower planning, at the national, industry, or company level. Certain recurring themes guided the selection of documents in this survey: (1) The main objective of macroeconomic planning is the guidance of changing unemployment patterns towards desired goals, (2) Manpower planning should be closely…

  1. Annotated bibliography traceability

    NARCIS (Netherlands)

    Narain, G.

    2006-01-01

    This annotated bibliography contains summaries of articles and chapters of books, which are relevant to traceability. After each summary there is a part about the relevancy of the paper for the LEI project. The aim of the LEI-project is to gain insight in several aspects of traceability in order to

  2. Defense Mechanisms: A Bibliography.

    Science.gov (United States)

    Pedrini, D. T.; Pedrini, Bonnie C.

    This bibliography includes studies of defense mechanisms, in general, and studies of multiple mechanisms. Defense mechanisms, briefly and simply defined, are the unconscious ego defendants against unpleasure, threat, or anxiety. Sigmund Freud deserves the clinical credit for studying many mechanisms and introducing them in professional literature.…

  3. Bibliography on Career Education.

    Science.gov (United States)

    Bridgeport Public Schools, CT.

    A research bibliography for use by persons involved in implementing career education concepts, the document contains all of the commercially produced multimedia used by Connecticut's exemplary project in career education. The resources listed include books, films, filmstrips, records, cassettes, games, picture story study prints, kits, and…

  4. ENGINES: exploring single nucleotide variation in entire human genomes

    Directory of Open Access Journals (Sweden)

    Salas Antonio

    2011-04-01

    Full Text Available Abstract Background Next generation ultra-sequencing technologies are starting to produce extensive quantities of data from entire human genome or exome sequences, and therefore new software is needed to present and analyse this vast amount of information. The 1000 Genomes project has recently released raw data for 629 complete genomes representing several human populations through their Phase I interim analysis and, although there are certain public tools available that allow exploration of these genomes, to date there is no tool that permits comprehensive population analysis of the variation catalogued by such data. Description We have developed a genetic variant site explorer able to retrieve data for Single Nucleotide Variation (SNVs, population by population, from entire genomes without compromising future scalability and agility. ENGINES (ENtire Genome INterface for Exploring SNVs uses data from the 1000 Genomes Phase I to demonstrate its capacity to handle large amounts of genetic variation (>7.3 billion genotypes and 28 million SNVs, as well as deriving summary statistics of interest for medical and population genetics applications. The whole dataset is pre-processed and summarized into a data mart accessible through a web interface. The query system allows the combination and comparison of each available population sample, while searching by rs-number list, chromosome region, or genes of interest. Frequency and FST filters are available to further refine queries, while results can be visually compared with other large-scale Single Nucleotide Polymorphism (SNP repositories such as HapMap or Perlegen. Conclusions ENGINES is capable of accessing large-scale variation data repositories in a fast and comprehensive manner. It allows quick browsing of whole genome variation, while providing statistical information for each variant site such as allele frequency, heterozygosity or FST values for genetic differentiation. Access to the data mart

  5. The Humanistic Side of Engineering: Considering Social Science and Humanities Dimensions of Engineering in Education and Research

    Science.gov (United States)

    Hynes, Morgan; Swenson, Jessica

    2013-01-01

    Mathematics and science knowledge/skills are most commonly associated with engineering's pre-requisite knowledge. Our goals in this paper are to argue for a more systematic inclusion of social science and humanities knowledge in the introduction of engineering to K-12 students. As part of this argument, we present a construct for framing the…

  6. The Pajarito Plateau: a bibliography

    Science.gov (United States)

    Mathien, Frances Joan; Steen, Charlie R.; Allen, Craig D.

    1993-01-01

    This bibliography is the result of two initially independent projects. As the consulting archaeologist at Los Alamos National Laboratory (LANL), Charlie R. Steen collected entries at the suggestion of the staff of the Environmental Surveillance Group of the Health, Safety, and Environmental Division, HSE-8. The primary purpose was to aid the staff in evaluating cultural resources on LANL lands. In addition to works that related to the archaeology and history of the area, Steen included notations of a few books and articles in other fields such as geology and natural history. It was hoped that they also would be of value to other organizations and to students of past human activities on the Pajarito Plateau.At the same time, the National Park Service (NPS) was planning a major survey of Bandelier National Monument (BNM). As part of this plan, the author was asked to prepare a background document that described research previously carried out in the area, including an annotated bibliography. Although the survey would be limited to the park boundaries, the larger Pajarito Plateau is a more logical study area from physiographic, environmental, and cultural perspectives; hence the focus was on this larger region. Mathien (1986) also included some references to natural resources studies, particularly those initiated by NPS within Bandelier National Monument.Both bibliographies were made available to Colleen Olinger and Beverly Larson of the Health and Environmental Services Group at Los Alamos. They realized that while neither was complete, each included entries missing from the other. Larson suggested the two bibliographies be combined. (At this time, Craig Allen was studying the landscape of the Jemez Mountains [Allen 1984c, 1989]. His investigations included much detailed information on natural resource studies and were added in 1991 and 1992.)To limit the scope of their work, Steen and Mathien had chosen their parameter: the Pajarito Plateau. Geographically, the

  7. 2015 Space Human Factors Engineering Standing Review Panel

    Science.gov (United States)

    Steinberg, Susan

    2015-01-01

    The 2015 Space Human Factors Engineering (SHFE) Standing Review Panel (from here on referred to as the SRP) met for a site visit in Houston, TX on December 2 - 3, 2015. The SRP reviewed the updated research plans for the Risk of Inadequate Design of Human and Automation/Robotic Integration (HARI Risk), the Risk of Inadequate Human-Computer Interaction (HCI Risk), and the Risk of Inadequate Mission, Process and Task Design (MPTask Risk). The SRP also received a status update on the Risk of Incompatible Vehicle/Habitat Design (Hab Risk) and the Risk of Performance Errors Due to Training Deficiencies (Train Risk). The SRP is pleased with the progress and responsiveness of the SHFE team. The presentations were much improved this year. The SRP is also pleased with the human-centered design approach. Below are some of the more extensive comments from the SRP. We have also made comments in each section concerning gaps/tasks in each. The comments below reflect more significant changes that impact more than just one particular section.

  8. Engineer Ethics Education that Treated Safety Problem to Promote Development of General Human Competence and Independent Engineer

    Science.gov (United States)

    Hotta, Genji

    The human race came to expect the engineer‧s international activity by an international extension of the risk. The engineer should provide with “Overall ability” and “Independent ability” to answer the demand of the society. The engineer ethics education is effective to the acquisition of the ability that the society demands. Because the engineer ethics education teaches the engineer to develop “Ethics action as the individual” to “Ethics activity as the enterprise” . In the point of development of the comprehensive capacity, it can be said that the engineer ethics education is training that supports the action power that accomplishes the social responsibility. However, it is easy to make the engineer ethics education a polite fiction. Then, we propose to take the safety problem to the ethics education for the prevention of making to the polite fiction of the education.

  9. TWRS privatization bibliography

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-02-01

    The purpose of this bibliography is to present a select set of documents that may be of interest to the Offeror, covering a variety of subject areas related to the TWRS Privatization Project. The organization of this bibliography is by subject area. Some of the documents overlap subject areas, and may be presented in more than one. Additionally, assignment of a document to one subject area does not necessarily preclude that document from containing information relevant to other subject areas not identified. The subject areas include, in order of presentation:. Waste Characterization; Pre-treatment; High-level Waste Immobilization; Low-level Waste Immobilization; Low-level Waste Melter Test Program; Performance Assessment; and General Safety.

  10. Uranium hexafluoride bibliography

    Energy Technology Data Exchange (ETDEWEB)

    Burnham, S.L.

    1988-01-01

    This bibliography is a compilation of reports written about the transportation, handling, safety, and processing of uranium hexafluoride. An on-line literature search was executed using the DOE Energy files and the Nuclear Science Abstracts file to identify pertinent reports. The DOE Energy files contain unclassified information that is processed at the Office of Scientific and Technical Information of the US Department of Energy. The reports selected from these files were published between 1974 and 1983. Nuclear Science Abstracts contains unclassified international nuclear science and technology literature published from 1948 to 1976. In addition, scientific and technical reports published by the US Atomic Energy Commission and the US Energy Research and Development Administration, as well as those published by other agencies, universities, and industrial and research organizations, are included in the Nuclear Science Abstracts file. An alphabetical listing of the acronyms used to denote the corporate sponsors follows the bibliography.

  11. Radon: a bibliography

    Energy Technology Data Exchange (ETDEWEB)

    Lepman, S.R.; Boegel, M.L.; Hollowell, C.D.

    1981-01-01

    The Lawrence Berkeley Laboratory, with the support of the Department of Energy, has developed a computerized database to manage research information in the area of building ventilation and indoor air quality. This literature survey contains references pertaining to the physical properties of radon and its daughters, instrumentation for their measurement, health effects, surveys and measurements, and regulatory information. The references in the bibliography are sequenced in alphabetical order and abstracts are included when supplied by the author. The objective of this report is to disseminate the bibliographic references compiled at the laboratory relating to radon research portion of the program. Interested database users are encouraged to contact the laboratory to receive instructions for direct database acess. A flyer describing the database is supplied at the end of the bibliography and a brief overview of the Radon Research porgram is given.

  12. Reverse engineering human neurodegenerative disease using pluripotent stem cell technology.

    Science.gov (United States)

    Liu, Ying; Deng, Wenbin

    2016-05-01

    With the technology of reprogramming somatic cells by introducing defined transcription factors that enables the generation of "induced pluripotent stem cells (iPSCs)" with pluripotency comparable to that of embryonic stem cells (ESCs), it has become possible to use this technology to produce various cells and tissues that have been difficult to obtain from living bodies. This advancement is bringing forth rapid progress in iPSC-based disease modeling, drug screening, and regenerative medicine. More and more studies have demonstrated that phenotypes of adult-onset neurodegenerative disorders could be rather faithfully recapitulated in iPSC-derived neural cell cultures. Moreover, despite the adult-onset nature of the diseases, pathogenic phenotypes and cellular abnormalities often exist in early developmental stages, providing new "windows of opportunity" for understanding mechanisms underlying neurodegenerative disorders and for discovering new medicines. The cell reprogramming technology enables a reverse engineering approach for modeling the cellular degenerative phenotypes of a wide range of human disorders. An excellent example is the study of the human neurodegenerative disease amyotrophic lateral sclerosis (ALS) using iPSCs. ALS is a progressive neurodegenerative disease characterized by the loss of upper and lower motor neurons (MNs), culminating in muscle wasting and death from respiratory failure. The iPSC approach provides innovative cell culture platforms to serve as ALS patient-derived model systems. Researchers have converted iPSCs derived from ALS patients into MNs and various types of glial cells, all of which are involved in ALS, to study the disease. The iPSC technology could be used to determine the role of specific genetic factors to track down what's wrong in the neurodegenerative disease process in the "disease-in-a-dish" model. Meanwhile, parallel experiments of targeting the same specific genes in human ESCs could also be performed to control

  13. Bibliographies africanistes courantes: une comparaison

    Directory of Open Access Journals (Sweden)

    M. d'Hertefelt

    1991-01-01

    Full Text Available Five internationally used current bibliographies are here compared with respect to their coverage of the periodical literature, the degree of their overlapping, and three basic aspects of their layout (list of analyzed periodicals, arrangement of references, index construction. As regards coverage, the most striking conclusion is the low to minimal rate of overlapping, so that it would be rather risky for the student to rely on one bibliography only, even the most extensive. Coverage, of course, is not the sole factor in assessing the value of a bibliography. The five bibliographies differ widely in their organisation and are by far not equally easy to use.

  14. Canadian petroleum history bibliography

    Energy Technology Data Exchange (ETDEWEB)

    Cass, D.

    2003-09-27

    The Petroleum History Bibliography includes a list of more than 2,000 publications that record the history of the Canadian petroleum industry. The list includes books, theses, films, audio tapes, published articles, company histories, biographies, autobiographies, fiction, poetry, humour, and an author index. It was created over a period of several years to help with projects at the Petroleum History Society. It is an ongoing piece of work, and as such, invites comments and additions.

  15. Geothermal resources in Arizona: a bibliography. Circular 23

    Energy Technology Data Exchange (ETDEWEB)

    Calvo, S.S.

    1982-01-01

    This bibliography references all reports and maps generated by the Arizona Bureau of Geology and Mineral Technology and the Arizona Geothermal Commercialization Team of the Department of Chemical Engineering, University of Arizona. To provide a more comprehensive listing of geothermal energy in Arizona, all available geothermal papers from other sources have been included. A total of 224 references are presented. (MHR)

  16. The Chandra Bibliography Database

    Science.gov (United States)

    Rots, A. H.; Winkelman, S. L.; Paltani, S.; Blecksmith, S. E.; Bright, J. D.

    2004-07-01

    Early in the mission, the Chandra Data Archive started the development of a bibliography database, tracking publications in refereed journals and on-line conference proceedings that are based on Chandra observations, allowing our users to link directly to articles in the ADS from our archive, and to link to the relevant data in the archive from the ADS entries. Subsequently, we have been working closely with the ADS and other data centers, in the context of the ADEC-ITWG, on standardizing the literature-data linking. We have also extended our bibliography database to include all Chandra-related articles and we are also keeping track of the number of citations of each paper. Obviously, in addition to providing valuable services to our users, this database allows us to extract a wide variety of statistical information. The project comprises five components: the bibliography database-proper, a maintenance database, an interactive maintenance tool, a user browsing interface, and a web services component for exchanging information with the ADS. All of these elements are nearly mission-independent and we intend make the package as a whole available for use by other data centers. The capabilities thus provided represent support for an essential component of the Virtual Observatory.

  17. Human serum provided additional values in growth factors supplemented medium for human chondrocytes monolayer expansion and engineered cartilage construction.

    Science.gov (United States)

    Chua, K H; Aminuddin, B S; Fuzina, N H; Ruszymah, B H I

    2004-05-01

    We have previously formulated an optimized human chondrocytes growth medium based on 2% fetal bovine serum supplementation. For clinical usage, the animal serum must be replaced by patient own serum. We investigated the effects of human serum concentration for human nasal septum chondrocytes monolayer culture and cartilage reconstruction. Human serum demonstrated a dose dependent manner in promoting chondrocytes growth and cartilage engineering.

  18. Medizinbibliothekarische Bibliographie 2005 / The Medical Librarian's Bibliography 2005

    Directory of Open Access Journals (Sweden)

    Bauer, Bruno

    2006-09-01

    Full Text Available The Medical Librarian’s Bibliography 2005 lists all articles from medizin - bibliothek - information and selected publications relevant to medical librarians from following journals: ABI Technik, Bibliothek Forschung & Praxis, Bibliotheksdienst, BIT online, Journal of the European Association for Health Information and Libraries, Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare, nfd, Online Mitteilungen, Zeitschrift für Bibliothekswesen & Bibliographie.

  19. Efficient derivation and genetic modifications of human pluripotent stem cells on engineered human feeder cell lines.

    Science.gov (United States)

    Zou, Chunlin; Chou, Bin-Kuan; Dowey, Sarah N; Tsang, Kitman; Huang, Xiaosong; Liu, Cyndi F; Smith, Cory; Yen, Jonathan; Mali, Prashant; Zhang, Yu Alex; Cheng, Linzhao; Ye, Zhaohui

    2012-08-10

    Derivation of pluripotent stem cells (iPSCs) induced from somatic cell types and the subsequent genetic modifications of disease-specific or patient-specific iPSCs are crucial steps in their applications for disease modeling as well as future cell and gene therapies. Conventional procedures of these processes require co-culture with primary mouse embryonic fibroblasts (MEFs) to support self-renewal and clonal growth of human iPSCs as well as embryonic stem cells (ESCs). However, the variability of MEF quality affects the efficiencies of all these steps. Furthermore, animal sourced feeders may hinder the clinical applications of human stem cells. In order to overcome these hurdles, we established immortalized human feeder cell lines by stably expressing human telomerase reverse transcriptase, Wnt3a, and drug resistance genes in adult mesenchymal stem cells. Here, we show that these immortalized human feeders support efficient derivation of virus-free, integration-free human iPSCs and long-term expansion of human iPSCs and ESCs. Moreover, the drug-resistance feature of these feeders also supports nonviral gene transfer and expression at a high efficiency, mediated by piggyBac DNA transposition. Importantly, these human feeders exhibit superior ability over MEFs in supporting homologous recombination-mediated gene targeting in human iPSCs, allowing us to efficiently target a transgene into the AAVS1 safe harbor locus in recently derived integration-free iPSCs. Our results have great implications in disease modeling and translational applications of human iPSCs, as these engineered human cell lines provide a more efficient tool for genetic modifications and a safer alternative for supporting self-renewal of human iPSCs and ESCs.

  20. Tissue-engineered microenvironment systems for modeling human vasculature.

    Science.gov (United States)

    Tourovskaia, Anna; Fauver, Mark; Kramer, Gregory; Simonson, Sara; Neumann, Thomas

    2014-09-01

    The high attrition rate of drug candidates late in the development process has led to an increasing demand for test assays that predict clinical outcome better than conventional 2D cell culture systems and animal models. Government agencies, the military, and the pharmaceutical industry have started initiatives for the development of novel in-vitro systems that recapitulate functional units of human tissues and organs. There is growing evidence that 3D cell arrangement, co-culture of different cell types, and physico-chemical cues lead to improved predictive power. A key element of all tissue microenvironments is the vasculature. Beyond transporting blood the microvasculature assumes important organ-specific functions. It is also involved in pathologic conditions, such as inflammation, tumor growth, metastasis, and degenerative diseases. To provide a tool for modeling this important feature of human tissue microenvironments, we developed a microfluidic chip for creating tissue-engineered microenvironment systems (TEMS) composed of tubular cell structures. Our chip design encompasses a small chamber that is filled with an extracellular matrix (ECM) surrounding one or more tubular channels. Endothelial cells (ECs) seeded into the channels adhere to the ECM walls and grow into perfusable tubular tissue structures that are fluidically connected to upstream and downstream fluid channels in the chip. Using these chips we created models of angiogenesis, the blood-brain barrier (BBB), and tumor-cell extravasation. Our angiogenesis model recapitulates true angiogenesis, in which sprouting occurs from a "parent" vessel in response to a gradient of growth factors. Our BBB model is composed of a microvessel generated from brain-specific ECs within an ECM populated with astrocytes and pericytes. Our tumor-cell extravasation model can be utilized to visualize and measure tumor-cell migration through vessel walls into the surrounding matrix. The described technology can be used

  1. Canadian petroleum history bibliography. Release update

    Energy Technology Data Exchange (ETDEWEB)

    Cass, D.

    2010-01-07

    The petroleum history bibliography was created over several years as a record dedicated to preserving the history of the Canadian petroleum industry. It comprises a list of more than 5000 publications, including books, theses, films, audio tapes, published articles and stories of the many companies that have come and gone. It aims to include all publications and audio visual products from the Social Sciences and Humanities on company histories, biographies, autobiographies, fiction, poetry and humour. An author index is included. Most government documents are excluded as they are accessible through Library and Archives Canada. This bibliography is an ongoing piece of work, and welcomes any additions relating to the study and preservation of Canadian petroleum industry history.

  2. Selected Nanny Child Care Bibliography.

    Science.gov (United States)

    Olsen, Glenn W., Comp.; Williams, Owen E., Comp.

    This bibliography on nanny child care contains approximately 180 references to material about nannies, mother's helpers, au pairs, and governesses. The bibliography is organized by the categories of newspaper articles, journal articles, newsletter articles, books, and audiovisual materials. The items are drawn primarily from U.S. sources, but some…

  3. War Termination: A Selected Bibliography

    Science.gov (United States)

    2010-10-01

    Peace Research 33, no. 4 (November 1996): 491-496. JSTOR Phinney, Catherine. "Enhancing Conflict Termination through Problem Solving...96." Journal of Peace Research 34, no. 3 (August 1997): 339-358. JSTOR Bibliographies Bibliography Branch, comp. Conflict Termination

  4. Strategic Vision: A Selected Bibliography

    Science.gov (United States)

    2008-03-01

    Journal 19 (Spring 2005): 59-66. ProQuest 27 BIBLIOGRAPHIES Costa , Mark R., comp. Transformation: A Selected Bibliography. Carlisle Barracks: U.S. Army War...SecurityStrategy07.pdf Phillips, Joan T., comp. Future. Maxwell Air Force Base: Muir S. Fairchild Research Information Center, November 2006. http

  5. Environmental Pollution, A Selective Bibliography.

    Science.gov (United States)

    Clearinghouse for Federal Scientific and Technical Information, Springfield, VA.

    This bibliography on environmental pollution is comprised primarily of reports of federally sponsored research by such departments and agencies as HEW, Interior, AEC, NASA, Defense, Transportation, and others. The reports were selected from Clearinghouse announcement journals during the period 1968 through April 1970. The bibliography contains…

  6. Modern Japanese Novels in English: A Selected Bibliography. Service Center Paper on Asian Studies, No. 7.

    Science.gov (United States)

    Beauchamp, Nancy Junko

    Selected contemporary Japanese novels translated into English are compiled in this bibliography as a guide for teachers interested in the possibilities offered by Japanese fiction. The bibliography acquaints teachers with available Japanese fiction that can be incorporated into social sciences or humanities courses to introduce Japan to students…

  7. Partially annotated bibliography for computer protection and related topics

    Energy Technology Data Exchange (ETDEWEB)

    Huskamp, J.C.

    1976-07-20

    References for the commonly cited technical papers in the area of computer protection are given. Great care is taken to exclude papers with no technical content or merit. For the purposes of this bibliography, computer protection is broadly defined to encompass all facets of the protection problem. The papers cover, but are not limited to, the topics of protection features in operating systems (e.g., MULTICS and HYDRA), hardware implementations of protection facilities (e.g., Honeywell 6180, System 250, BCC 5000, B6500), data base protection controls, confinement and protection models. Since computer protection is related to many other areas in computer science and electrical engineering, a bibliography of related areas is included after the protection bibliography. These sections also include articles of general interest in the named areas which are not necessarily related to protection.

  8. Human Engineering Design Criteria for Modern Control/Display Components and Standard Parts.

    Science.gov (United States)

    1980-05-01

    Journal of Ergonomics and the Human Factors Journal indexes were reviewed. DDC and NASA STIF data bases were also searched. Human engineers specializing...addition, the Journal of Ergonomics and the Human Factors Journal indexes were reviewed. DDC and NASA STIF data bases were also searched. Human

  9. Bibliography for Advancement Study.

    Science.gov (United States)

    1987-07-01

    Uses, NAVEDTRA 10085-BI 1L SW 3&2, NAVEDTRA 10653-Fl -- Chaps 5, 8, 9, 0 10, 14, 15, and 16 Principles of Automotive Vehicles, TM 9-8000 Navy Driver’s...000 Chap 083, Allowances, Issues, Expeditures of ,.~ Material and Repair Parts, NAVSEA S9086-CS-STM-000 ~. ENCS (EN3, EN2, ENi, AND ENC BIBLIOGRAPHIES...TRAININ SERIES ____ M, JULE TITLE NAVEDTRA N’ir %,% %. %𔃻 Introduction to MPatter, Energy , and Direct Current %17Z-31-30-79 0507-Li- - {jCC-ECC

  10. Fuel cells: A bibliography

    Science.gov (United States)

    1988-05-01

    This bibliography includes 1392 citations of domestic and foreign reports, journal articles, patents, conference papers and proceedings and books. The citations were taken from the DOE Energy Data Base covering the period March 1985 through February 1988. These citations are arranged in the following six subject categories: general; design and development; performance testing; materials, components, and auxiliaries; applications; and electrochemistry, mass transfer, and thermodynamics. Within each category, the report citations are arranged alphanumerically by report numbers, and nonreport literature citations are arranged in reverse chronological order. Indexes are provided for corporate author, personal author, subject, contract number, and report number.

  11. Human fetal bone cells in delivery systems for bone engineering.

    Science.gov (United States)

    Tenorio, Diene M H; Scaletta, Corinne; Jaccoud, Sandra; Hirt-Burri, Nathalie; Pioletti, Dominique P; Jaques, Bertrand; Applegate, Lee Ann

    2011-11-01

    The aim of this study was to culture human fetal bone cells (dedicated cell banks of fetal bone derived from 14 week gestation femurs) within both hyaluronic acid gel and collagen foam, to compare the biocompatibility of both matrices as potential delivery systems for bone engineering and particularly for oral application. Fetal bone cell banks were prepared from one organ donation and cells were cultured for up to 4 weeks within hyaluronic acid (Mesolis®) and collagen foams (TissueFleece®). Cell survival and differentiation were assessed by cell proliferation assays and histology of frozen sections stained with Giemsa, von Kossa and ALP at 1, 2 and 4 weeks of culture. Within both materials, fetal bone cells could proliferate in three-dimensional structure at ∼70% capacity compared to monolayer culture. In addition, these cells were positive for ALP and von Kossa staining, indicating cellular differentiation and matrix production. Collagen foam provides a better structure for fetal bone cell delivery if cavity filling is necessary and hydrogels would permit an injectable technique for difficult to treat areas. In all, there was high biocompatibility, cellular differentiation and matrix deposition seen in both matrices by fetal bone cells, allowing for easy cell delivery for bone stimulation in vivo. Copyright © 2011 John Wiley & Sons, Ltd.

  12. Health effects of mustard gas and lewisite: Subject bibliography

    Energy Technology Data Exchange (ETDEWEB)

    Liverman, C.T.; Pechura, C.M.

    1992-12-01

    The subject bibliography covers the scientific, military, and technical literature written on animal studies and human exposure to the chemical warfare agents, sulfur mustard (mustard gas) and Lewisite. Primarily structured by organ system, the human studies are also organized by the type of exposure (i.e. combat, occupational, and experimental). The bibliography was compiled during the work of the Institute of Medicine's Committee to Survey the Health Effects of Mustard Gas and Lewisite and includes sources on the historical use of chemical warfare agents and on related chemical compounds including the nitrogen mustards.

  13. Sabina Spielrein: a bibliography.

    Science.gov (United States)

    Allain-Dupré, Brigitte

    2004-06-01

    The work on this bibliography was carried out mostly in 2002. Though it aims at being exhaustive, the research in different languages and translations may not have yielded every document and some may still be unknown to me. It is also likely that more material have been translated since. Besides, the Spanish and Brazilian-Portuguese literature has not been researched. This is why this bibliography must be considered as a 'work in progress' which any addition can only enrich. Victor Ovcharenko has made a list of the writings of Sabina Spielrein starting with 1911, the year of her medical thesis in Zürich, and covering the years to 1931. He points out, however, that between the date of the last paper that she is known to have written and her death in 1942, there are eleven years and that it would be truly astonishing if she had neither written nor published anything during that period. Up to this date no document by her during that time has been found (Journal of Analytical Psychology, 44, 3, 1999).

  14. TWRS privatization bibliography. Revision 2

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-04-01

    The purpose of this bibliography is to present a select set of documents that may be of interest to the Offeror, covering a variety of subject areas related to the TWRS Privatization Project. This bibliography is not, nor is it intended to be, exhaustive or complete. It was prepared with the intent of providing a sampling of representative documents potentially helpful to Offerors. This bibliography is organized by subject area. The subjects covered are: waste characterization; pre-treatment; high-level waste immobilization; low-level waste immobilization; low-level waste melter test program; performance assessment; general; and safety.

  15. Human resource management engineering companies Ukraine under crisis

    Directory of Open Access Journals (Sweden)

    Redkva, Oksana Zinoviyivna

    2011-11-01

    Full Text Available The features of personal management at engineering enterprises in the crisis are describes and discuses in the article. The problems and causes of inefficient personnel management are identified. Jome recommendations concerming solving of problem in the sphere of personal management and effective management of engineering enterprises are giver.

  16. Experiences in the application of human factors engineering to human-system interface modernization

    Energy Technology Data Exchange (ETDEWEB)

    Trueba Alonso, Pedro; Fernandez Illobre, Luis; Ortega Pascual, Fernando [Tecnatom S.A., San Sebastian de los Reyes (Spain). Simulation and Control Rooms Div.

    2015-07-15

    Almost all the existing Nuclear Power Plants (NPPs) include plans to modernize their existing Instrumentation and Control (I and C) systems and associated Human System Interfaces (HSIs), due to obsolescence problems. Tecnatom, S.A. has been participating in modernization programs in NPPs to help them to plan, specify, design and implement the modernization of control rooms and associated I and C and HSIs. The application of Human Factors Engineering (HFE) in modernization programs is nowadays unavoidable. This is because is becoming a regulatory requirement, and also because it is needed to ensure that any plant modification, involving the modernization of I and C and HSI, is well designed to improve overall plant operations, reliability, and safety. This paper shows some experiences obtained during the application of HFE to the modernization of these HSIs. The experience applying HFE in modernizations and design modifications show a positive effect, improving the associated HSIs, with the acceptability of the final user.

  17. Unifying Human Centered Design and Systems Engineering for Human Systems Integration

    Science.gov (United States)

    Boy, Guy A.; McGovernNarkevicius, Jennifer

    2013-01-01

    Despite the holistic approach of systems engineering (SE), systems still fail, and sometimes spectacularly. Requirements, solutions and the world constantly evolve and are very difficult to keep current. SE requires more flexibility and new approaches to SE have to be developed to include creativity as an integral part and where the functions of people and technology are appropriately allocated within our highly interconnected complex organizations. Instead of disregarding complexity because it is too difficult to handle, we should take advantage of it, discovering behavioral attractors and the emerging properties that it generates. Human-centered design (HCD) provides the creativity factor that SE lacks. It promotes modeling and simulation from the early stages of design and throughout the life cycle of a product. Unifying HCD and SE will shape appropriate human-systems integration (HSI) and produce successful systems.

  18. Cartilage tissue engineering using pre-aggregated human articular chondrocytes

    Directory of Open Access Journals (Sweden)

    F Wolf

    2008-12-01

    Full Text Available In this study, we first aimed at determining whether human articular chondrocytes (HAC proliferate in aggregates in the presence of strong chondrocyte mitogens. We then investigated if the aggregated cells have an enhanced chondrogenic capacity as compared to cells cultured in monolayer. HAC from four donors were cultured in tissue culture dishes either untreated or coated with 1% agarose in the presence of TGFb-1, FGF-2 and PDGF-BB. Proliferation and stage of differentiation were assessed by measuring respectively DNA contents and type II collagen mRNA. Expanded cells were induced to differentiate in pellets or in Hyaff®-11 meshes and the formed tissues were analysed biochemically for glycosaminoglycans (GAG and DNA, and histologically by Safranin O staining. The amount of DNA in aggregate cultures increased significantly from day 2 to day 6 (by 3.2-fold, but did not further increase with additional culture time. Expression of type II collagen mRNA was about two orders of magnitude higher in aggregated HAC as compared to monolayer expanded cells. Pellets generated by aggregated HAC were generally more intensely stained for GAG than those generated by monolayer-expanded cells. Scaffolds seeded with aggregates accumulated more GAG (1.3-fold than scaffolds seeded with monolayer expanded HAC. In conclusion, this study showed that HAC culture in aggregates does not support a relevant degree of expansion. However, aggregation of expanded HAC prior to loading into a porous scaffold enhances the quality of the resulting tissues and could thus be introduced as an intermediate culture phase in the manufacture of engineered cartilage grafts.

  19. Social Engineering: I-E based Model of Human Weakness for Attack and Defense Investigations

    Directory of Open Access Journals (Sweden)

    Wenjun Fan

    2017-01-01

    Full Text Available Social engineering is the attack aimed to manipulate dupe to divulge sensitive information or take actions to help the adversary bypass the secure perimeter in front of the information-related resources so that the attacking goals can be completed. Though there are a number of security tools, such as firewalls and intrusion detection systems which are used to protect machines from being attacked, widely accepted mechanism to prevent dupe from fraud is lacking. However, the human element is often the weakest link of an information security chain, especially, in a human-centered environment. In this paper, we reveal that the human psychological weaknesses result in the main vulnerabilities that can be exploited by social engineering attacks. Also, we capture two essential levels, internal characteristics of human nature and external circumstance influences, to explore the root cause of the human weaknesses. We unveil that the internal characteristics of human nature can be converted into weaknesses by external circumstance influences. So, we propose the I-E based model of human weakness for social engineering investigation. Based on this model, we analyzed the vulnerabilities exploited by different techniques of social engineering, and also, we conclude several defense approaches to fix the human weaknesses. This work can help the security researchers to gain insights into social engineering from a different perspective, and in particular, enhance the current and future research on social engineering defense mechanisms.

  20. New dimensions in tissue engineering: possible models for human physiology.

    Science.gov (United States)

    Baar, Keith

    2005-11-01

    Tissue engineering is a discipline of great promise. In some areas, such as the cornea, tissues engineered in the laboratory are already in clinical use. In other areas, where the tissue architecture is more complex, there are a number of obstacles to manoeuvre before clinically relevant tissues can be produced. However, even in areas where clinically relevant tissues are decades away, the tissues being produced at the moment provide powerful new models to aid the understanding of complex physiological processes. This article provides a personal view of the role of tissue engineering in advancing our understanding of physiology, with specific attention being paid to musculoskeletal tissues.

  1. Engineering bone tissue substitutes from human induced pluripotent stem cells

    National Research Council Canada - National Science Library

    Giuseppe Maria de Peppo; Iván Marcos-Campos; David John Kahler; Dana Alsalman; Linshan Shang; Gordana Vunjak-Novakovic; Darja Marolt

    2013-01-01

    ...) for bone tissue engineering. We first induced three hiPSC lines with different tissue and reprogramming backgrounds into the mesenchymal lineages and used a combination of differentiation assays, surface antigen profiling...

  2. Ideas in Practice: The Mississippi River: Humanities and Civil Engineering.

    Science.gov (United States)

    Vonalt, Larry; And Others

    1980-01-01

    Describes a course offered for the freshman civil engineering major at the University of Missouri-Rolla. The rationale of developing the course which focuses on the symbolic, social, and technological aspects of the Mississippi River is included. (HM)

  3. Towards the bibliography of life.

    Science.gov (United States)

    King, David; Morse, David R; Willis, Alistair; Dil, Anton

    2011-01-01

    This paper discusses how we intend to take forward the vision of a Bibliography of Life in the ViBRANT project. The underlying principle of the Bibliography is to provide taxonomists and others with a freely accessible bibliography covering the whole of life. Such a bibliography has been achieved for specific study areas within taxonomy, but not for "life" as a whole.The creation of such a comprehensive tool has been hindered by various social and technical issues. The social concerns focus on the willingness of users to contribute to the Bibliography. The technical concerns relate to the architecture required to deliver the Bibliography. These issues are discussed in the paper and approaches to addressing them within the ViBRANT project are described, to demonstrate how we can now seriously consider building a Bibliography of Life. We are particularly interested in the potential of the resulting tool to improve the quality of bibliographic references. Through analysing the large number of references in the Bibliography we will be able to add metadata by resolving known issues such as geographical name variations. This should result in a tool that will assist taxonomists in two ways. Firstly, it will be easier for them to discover relevant literature, especially pre-digital literature; and secondly, it will be easier for them to identify the canonical form for a citationThe paper also covers related issues relevant to building the tool in ViBRANT, including implementation and copyright, with suggestions as to how we could address them.

  4. Training in Human Relations for Engineers at the Ecole Superieure D'Informatique-Electronique-Automatique (ESIEA).

    Science.gov (United States)

    Lafargue, M.; And Others

    1986-01-01

    Points out the need to provide engineers with training in human relations. Describes the process of developing a document defining the problem and steps to be taken toward solution, submitted to students for their evaluation. (JM)

  5. Training in Human Relations for Engineers at the Ecole Superieure D'Informatique-Electronique-Automatique (ESIEA).

    Science.gov (United States)

    Lafargue, M.; And Others

    1986-01-01

    Points out the need to provide engineers with training in human relations. Describes the process of developing a document defining the problem and steps to be taken toward solution, submitted to students for their evaluation. (JM)

  6. Phase 2 Clinical Trial of Intraoral Grafting of Human Tissue-Engineered Oral Mucosa

    Science.gov (United States)

    2013-10-01

    Engineered Oral Mucosa   PRINCIPAL INVESTIGATOR: Stephen E. Feinberg DDS, MS, PhD CONTRACTING ORGANIZATION: University of Michigan Ann Arbor MI 4810...September 29, 4. TITLE AND SUBTITLE Phase II Clinical Trial of Intraoral Grafting of Human Tissue-Engineered Oral Mucosa   5a. CONTRACT NUMBER...human EVPOME for soft tissue intraoral grafting procedures compared to the “gold standard” palatal oral mucosa (POM) graft. The study will determine

  7. Genetic engineering of human ES and iPS cells using TALE nucleases

    OpenAIRE

    Hockemeyer, Dirk; Wang, Haoyi; Kiani, Samira; Lai, Christine S.; Gao, Qing; Cassady, John P.; Cost, Gregory J.; Zhang, Lei; Santiago, Yolanda; Miller, Jeffrey C; Zeitler, Bryan; Cherone, Jennifer M.; Meng, Xiangdong; Hinkley, Sarah J; Rebar, Edward J.

    2011-01-01

    Targeted genetic engineering of human pluripotent cells is a prerequisite for exploiting their full potential. Such genetic manipulations can be achieved using site-specific nucleases. Here we engineered transcription activator–like effector nucleases (TALENs) for five distinct genomic loci. At all loci tested we obtained human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) clones carrying transgenic cassettes solely at the TALEN-specified location. Our data suggest that T...

  8. IN VITRO EFFECT OF SILVER ENGINEERED NANOPARTICLES ON HUMAN SPERMATOZOA

    Directory of Open Access Journals (Sweden)

    G. Terzuoli

    2012-08-01

    Full Text Available Silver (Ag NPs are among the most commercialized NPs due to their antimicrobial potential. They are highly at- tractive for possible applications in manufacture of medical device. However there is a serious lack of information concerning their impact on the human health and the environment. Moreover studies on the effects of NPs on ejaculated sperm are rather limited. For these reasons our study explored the in vitro effects of Ag NPs on human ejaculated spermatozoa. Ag NPs have been produced, characterized, and furnished by Colorobbia Industry, Sovigliana (Vinci, Florence, Italy. Aliquots of total semen were incubated at 37°C for 60 minutes (min and 120 min at the concentration of 125 μM, 250 μM, and 500 μM of engineered Ag NPs. The control was represented by specimens of semen samples treated with the same procedure without NPs. After the incubations, sperm motility was evaluated following WHO guidelines and sperm viability was eval- uated by Eosin Y test. At the end of incubation with Ag NPs the samples were processed by a Field Emission Gun-based Scanning Transmition Electron Microscope/ Energy Dispertion Spectrometry (STEM/EDS. We observed that sperm motility percentage was significantly reduced in semen samples treated with 125 μM, 250 μM and 500 μM of Ag NPs after 60 min and 120 min of incubation respect to controls (P<0.001; P<0.01, 125 μM at 60 min. Sperm viability percentage significantly decreased in a progressive manner after 125 μM (P<0.05, 250 μM (P<0.05 and 500 μM (P<0.001 Ag NPs incubation at 60 min and 120 min. We did not find any significant difference between the values assessed after 60 min of NPs incubation and those estimated after 120 min of incubation. In the control samples, the sperm motility and the sperm viability percentages significantly decreased after 120 min of incubation (P<0.001 respect to the basal values. Ag NPs were undetectable in all treated samples by STEM/EDS. These in vitro results show a

  9. A Bibliography of Software Engineering Terms.

    Science.gov (United States)

    1979-10-01

    ANSI-X3HI) 75 p 1 PARANORMAL TERMINATION UNSTRUCTURED ESCAPES (IN CONTROL) FROM A MODULE IN RESPONSE TO NORMAL EVENTS OR CONDITIONS. MODULES HAVING... PARANORMAL TERMINATIONS MAY YET EXHIBIT A FORM OF STRUCTURED CONTROL FLOW, IF PROPERLY CONFIGURED INTO " PARANORMAL EXTENSIONS" OF STRUCTURED PROGRAMMING

  10. Aeronautical Engineering. A Continuing Bibliography with Indexes

    Science.gov (United States)

    1987-09-01

    basis and a means FAHREN FUER REIBUNGSFREIE UND REIBUNGSBEHAFTETE of numerical simulation for further improving the design of turbine...tegrated with additional on-board sensors to yield a complete danger-warning systems and radio navigation and landing id autonomous navigation system. O.C...Examples of the use of such systems are described, including their application as autonomous assistants in cockpits and as man/machine interfaces in flight

  11. House dust mites in Brazil - an annotated bibliography

    Directory of Open Access Journals (Sweden)

    Binotti Raquel S

    2001-01-01

    Full Text Available House dust mites have been reported to be the most important allergen in human dwellings. Several articles had already shown the presence of different mite species at homes in Brazil, being Pyroglyphidae, Glycyphagidae and Cheyletidae the most important families found. This paper is an annotated bibliography that will lead to a better knowledge of house dust mite fauna in Brazil.

  12. Pertinent Discussions Toward Modeling the Social Edition: Annotated Bibliographies

    NARCIS (Netherlands)

    Siemens, R.; Timney, M.; Leitch, C.; Koolen, C.; Garnett, A.

    2012-01-01

    The two annotated bibliographies present in this publication document and feature pertinent discussions toward the activity of modeling the social edition, first exploring reading devices, tools and social media issues and, second, social networking tools for professional readers in the Humanities.

  13. Odors and Air Pollution: A Bibliography with Abstracts.

    Science.gov (United States)

    Environmental Protection Agency, Research Triangle Park, NC. Office of Air Programs.

    The annotated bibliography presents a compilation of abstracts which deal with odors as they relate to air pollution. The abstracts are arranged within the following categories: Emission sources; Control methods; Measurement methods; Air quality measurements; Atmospheric interaction; Basic science and technology; Effects-human health;…

  14. Education and Training. Annotated Bibliography. Author and Subject Index.

    Science.gov (United States)

    United Nations Food and Agriculture Organization, Rome (Italy).

    Food and Agriculture Organization (FAO) publications and documents issued by the Human Resources and Institutions division and by other technical divisions in the technical, economic, and social fields are selected, annotated and indexed in this bibliography. Documents issued prior to 1967 are not included but can be found in the Rural…

  15. Anthropometry. A Bibliography with Abstracts

    Science.gov (United States)

    1975-08-01

    performance items were o interrelationships among statistically significant ( between the muscle stren none between the range of The somatotype ...medical and psychological aspects of the U.S. naval aviator; Somatotyping ; Human factors in aircraft design. DESCRIPTORS: (*Anthropometry, *Hu«an...engineering), Aircraft cabins, Pilots, instrument panels, Cockpits, Aviation medicine, Naval personnel. Design IDENTIFIERS: Somatotyping AD-695

  16. Information Presentation: Human Research Program - Space Human Factors and Habitability, Space Human Factors Engineering Project

    Science.gov (United States)

    Holden, Kristina L.; Sandor, Aniko; Thompson, Shelby G.; Kaiser, Mary K.; McCann, Robert S.; Begault, D. R.; Adelstein, B. D.; Beutter, B. R.; Wenzel, E. M.; Godfroy, M.; Stone, L. S.

    2010-01-01

    The goal of the Information Presentation Directed Research Project (DRP) is to address design questions related to the presentation of information to the crew. The major areas of work, or subtasks, within this DRP are: 1) Displays, 2) Controls, 3) Electronic Procedures and Fault Management, and 4) Human Performance Modeling. This DRP is a collaborative effort between researchers atJohnson Space Center and Ames Research Center. T

  17. Approaches to Software Engineering: A Human-Centred Perspective

    OpenAIRE

    Bannon, Liam J.

    2010-01-01

    International audience; The field of software engineering has been evolving since its inception in 1968. Arguments as to the exact nature of the field, whether it should be conceived as a real engineering profession, the role of formal methods, whether it is as much an art as a science, etc., continue to divide both practitioners and academics. My purpose here is not to debate these particular topics, but rather to approach the field from the outside, coming as I do from a long period of invo...

  18. A HUMAN FACTORS ENGINEERING PROCESS TO SUPPORT HUMAN-SYSTEM INTERFACE DESIGN IN CONTROL ROOM MODERNIZATION

    Energy Technology Data Exchange (ETDEWEB)

    Kovesdi, C.; Joe, J.; Boring, R.

    2017-05-01

    The primary objective of the United States (U.S.) Department of Energy (DOE) Light Water Reactor Sustainability (LWRS) program is to sustain operation of the existing commercial nuclear power plants (NPPs) through a multi-pathway approach in conducting research and development (R&D). The Advanced Instrumentation, Information, and Control (II&C) System Technologies pathway conducts targeted R&D to address aging and reliability concerns with legacy instrumentation and control (I&C) and other information systems in existing U.S. NPPs. Control room modernization is an important part following this pathway, and human factors experts at Idaho National Laboratory (INL) have been involved in conducting R&D to support migration of new digital main control room (MCR) technologies from legacy analog and legacy digital I&C. This paper describes a human factors engineering (HFE) process that supports human-system interface (HSI) design in MCR modernization activities, particularly with migration of old digital to new digital I&C. The process described in this work is an expansion from the LWRS Report INL/EXT-16-38576, and is a requirements-driven approach that aligns with NUREG-0711 requirements. The work described builds upon the existing literature by adding more detail around key tasks and decisions to make when transitioning from HSI Design into Verification and Validation (V&V). The overall objective of this process is to inform HSI design and elicit specific, measurable, and achievable human factors criteria for new digital technologies. Upon following this process, utilities should have greater confidence with transitioning from HSI design into V&V.

  19. Film/Video Animation: A Bibliography.

    Science.gov (United States)

    Phipps, Steven P.

    Comprising approximately 475 items, this bibliography on film and video animation includes books and journal articles dating from 1917 to 1988. Both American and foreign sources are represented in the bibliography. (RS)

  20. Cultivating Engineers' Humanity: Fostering Cosmopolitanism in a Technical University

    Science.gov (United States)

    Boni, Alejandra; MacDonald, Penny; Peris, Jordi

    2012-01-01

    This paper aims to explore the potential of a curriculum designed to develop Nussbaum's cosmopolitan abilities through two elective subjects offered to future engineers in a Spanish Technical University. To this end, Nussbaum's proposition of cosmopolitan abilities is presented in relation to the broader academic literature on cosmopolitanism and…

  1. Musculoskeletal tissue engineering with human umbilical cord mesenchymal stromal cells

    Science.gov (United States)

    Wang, Limin; Ott, Lindsey; Seshareddy, Kiran; Weiss, Mark L; Detamore, Michael S

    2011-01-01

    Multipotent mesenchymal stromal cells (MSCs) hold tremendous promise for tissue engineering and regenerative medicine, yet with so many sources of MSCs, what are the primary criteria for selecting leading candidates? Ideally, the cells will be multipotent, inexpensive, lack donor site morbidity, donor materials should be readily available in large numbers, immunocompatible, politically benign and expandable in vitro for several passages. Bone marrow MSCs do not meet all of these criteria and neither do embryonic stem cells. However, a promising new cell source is emerging in tissue engineering that appears to meet these criteria: MSCs derived from Wharton’s jelly of umbilical cord MSCs. Exposed to appropriate conditions, umbilical cord MSCs can differentiate in vitro along several cell lineages such as the chondrocyte, osteoblast, adipocyte, myocyte, neuronal, pancreatic or hepatocyte lineages. In animal models, umbilical cord MSCs have demonstrated in vivo differentiation ability and promising immunocompatibility with host organs/tissues, even in xenotransplantation. In this article, we address their cellular characteristics, multipotent differentiation ability and potential for tissue engineering with an emphasis on musculoskeletal tissue engineering. PMID:21175290

  2. Human Resources Development in the Field of Electrical Engineering

    Science.gov (United States)

    Ishigame, Atsushi

    It is becoming increasingly clear that the decline in popularity in the field of electrical engineering is undergoing rapidly due to the fact that more young people are moving away from the science. The primary goal of this paper is to recognize the importance of educational new effort and, second, suggest social-provided education support needed to meet this challenge.

  3. Decellularization of human and porcine lung tissues for pulmonary tissue engineering.

    Science.gov (United States)

    O'Neill, John D; Anfang, Rachel; Anandappa, Annabelle; Costa, Joseph; Javidfar, Jeffrey; Wobma, Holly M; Singh, Gopal; Freytes, Donald O; Bacchetta, Matthew D; Sonett, Joshua R; Vunjak-Novakovic, Gordana

    2013-09-01

    The only definitive treatment for end-stage organ failure is orthotopic transplantation. Lung extracellular matrix (LECM) holds great potential as a scaffold for lung tissue engineering because it retains the complex architecture, biomechanics, and topologic specificity of the lung. Decellularization of human lungs rejected from transplantation could provide "ideal" biologic scaffolds for lung tissue engineering, but the availability of such lungs remains limited. The present study was designed to determine whether porcine lung could serve as a suitable substitute for human lung to study tissue engineering therapies. Human and porcine lungs were procured, sliced into sheets, and decellularized by three different methods. Compositional, ultrastructural, and biomechanical changes to the LECM were characterized. The suitability of LECM for cellular repopulation was evaluated by assessing the viability, growth, and metabolic activity of human lung fibroblasts, human small airway epithelial cells, and human adipose-derived mesenchymal stem cells over a period of 7 days. Decellularization with 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) showed the best maintenance of both human and porcine LECM, with similar retention of LECM proteins except for elastin. Human and porcine LECM supported the cultivation of pulmonary cells in a similar way, except that the human LECM was stiffer and resulted in higher metabolic activity of the cells than porcine LECM. Porcine lungs can be decellularized with CHAPS to produce LECM scaffolds with properties resembling those of human lungs, for pulmonary tissue engineering. We propose that porcine LECM can be an excellent screening platform for the envisioned human tissue engineering applications of decellularized lungs. Copyright © 2013 The Society of Thoracic Surgeons. Published by Elsevier Inc. All rights reserved.

  4. Human resource practices as predictors of engineering staff's organisational commitment

    National Research Council Canada - National Science Library

    Melinde Coetzee; Jeremy Mitonga-Monga; Benita Swart

    2014-01-01

      Human resource practices are an important means of retaining professionally qualified employees and improving and increasing their future level of organisational performance in today's turbulent...

  5. Mass Communication in Malaysia: An Annotated Bibliography.

    Science.gov (United States)

    Tee, Lim Huck, Comp; Sarachandran, V.V., Comp.

    This bibliography lists published and unpublished material relating to mass communications in Malaysia, 1945 to 1973. Most of the items listed are written in English and Malay, and a limited number are in Chinese. The bibliography is divided into 21 sections: bibliography and reference material; communication theory, research methods;…

  6. Romantic Poets and Prose Writers. Goldentree Bibliographies.

    Science.gov (United States)

    Fogle, Richard Harter, Comp.

    Intended as a guide to scholarship on Romantic writers, this selective bibliography attempts to cover the major works and topics, with emphasis on work published in the 20th century. Omitted are unpublished dissertations and most literary histories, bibliographies of bibliography, short notes and explications, and older biographical works that…

  7. Using human factors engineering to improve the effectiveness of infection prevention and control.

    Science.gov (United States)

    Anderson, Judith; Gosbee, Laura Lin; Bessesen, Mary; Williams, Linda

    2010-08-01

    Human factors engineering is a discipline that studies the capabilities and limitations of humans and the design of devices and systems for improved performance. The principles of human factors engineering can be applied to infection prevention and control to study the interaction between the healthcare worker and the system that he or she is working with, including the use of devices, the built environment, and the demands and complexities of patient care. Some key challenges in infection prevention, such as delayed feedback to healthcare workers, high cognitive workload, and poor ergonomic design, are explained, as is how human factors engineering can be used for improvement and increased compliance with practices to prevent hospital-acquired infections.

  8. Is Law a Humanity: (Or Is It More like Engineering)?

    Science.gov (United States)

    Howarth, David

    2004-01-01

    Law often appears to be in a limbo between the Social Sciences and the Humanities. Movements within legal scholarship itself, the law and economics movement and the law and literature movement, represent efforts to portray law as a social science or as a humanity. But if one looks at what lawyers do, one finds that law is more like…

  9. Reverse Engineering Human Pathophysiology with Organs-on-Chips.

    Science.gov (United States)

    Ingber, Donald E

    2016-03-10

    While studies of cultured cells have led to new insights into biological control, greater understanding of human pathophysiology requires the development of experimental systems that permit analysis of intercellular communications and tissue-tissue interactions in a more relevant organ context. Human organs-on-chips offer a potentially powerful new approach to confront this long-standing problem.

  10. Engineering Substantially Prolonged Human Lifespans: Biotechnological Enhancement and Ethics

    NARCIS (Netherlands)

    Derkx, P.H.J.M.

    2006-01-01

    Substantial extension of the human lifespan has recently become a subject of lively debate. One reason for this is the completion in 2001 of the Human Genome Project and the experimental avenues for biogerontological research it has opened. Another is recent theoretical progress in biogerontology.

  11. Human Research Program Space Human Factors Engineering (SHFE) Standing Review Panel (SRP)

    Science.gov (United States)

    Wichansky, Anna; Badler, Norman; Butler, Keith; Cummings, Mary; DeLucia, Patricia; Endsley, Mica; Scholtz, Jean

    2009-01-01

    The Space Human Factors Engineering (SHFE) Standing Review Panel (SRP) evaluated 22 gaps and 39 tasks in the three risk areas assigned to the SHFE Project. The area where tasks were best designed to close the gaps and the fewest gaps were left out was the Risk of Reduced Safety and Efficiency dire to Inadequate Design of Vehicle, Environment, Tools or Equipment. The areas where there were more issues with gaps and tasks, including poor or inadequate fit of tasks to gaps and missing gaps, were Risk of Errors due to Poor Task Design and Risk of Error due to Inadequate Information. One risk, the Risk of Errors due to Inappropriate Levels of Trust in Automation, should be added. If astronauts trust automation too much in areas where it should not be trusted, but rather tempered with human judgment and decision making, they will incur errors. Conversely, if they do not trust automation when it should be trusted, as in cases where it can sense aspects of the environment such as radiation levels or distances in space, they will also incur errors. This will be a larger risk when astronauts are less able to rely on human mission control experts and are out of touch, far away, and on their own. The SRP also identified 11 new gaps and five new tasks. Although the SRP had an extremely large quantity of reading material prior to and during the meeting, we still did not feel we had an overview of the activities and tasks the astronauts would be performing in exploration missions. Without a detailed task analysis and taxonomy of activities the humans would be engaged in, we felt it was impossible to know whether the gaps and tasks were really sufficient to insure human safety, performance, and comfort in the exploration missions. The SRP had difficulty evaluating many of the gaps and tasks that were not as quantitative as those related to concrete physical danger such as excessive noise and vibration. Often the research tasks for cognitive risks that accompany poor task or

  12. Establishment and characterization of human engineered cells stably expressing large extracellular matrix proteins.

    Science.gov (United States)

    Kwon, Daekee; Kang, Gwang-Sik; Han, Dong Keun; Park, Kwideok; Kim, Jae-Hwan; Lee, Soo-Hong

    2014-01-01

    Commercially available extracellular matrix (ECM) hydrogel-coated culture plates have been used to study the relationship between the ECM microenvironment and stem cell behavior. However, it is unclear whether ECM-coated dishes mimic the natural ECM microenvironment because the architecture of the ECM is constructed of randomly distributed fibers. The purpose of this study was the production and confirmation of human engineered cell lines stably expressing large ECM proteins such as collagen I/II and fibronectin. First, large (over 10 kb) ECM vectors encoding human collagen I/II and fibronectin were constructed and the circular vectors were linearized. Second, the linear ECM vectors were introduced into immortalized human embryonic kidney cells using various transfection methods. The polyethylenimine and liposome methods showed higher efficiencies than electroporation for transfection of these large vectors. Third, human ECM engineered cells were established by stable integration of the vector into the genomic DNA and resulted in stable overexpression of mRNA and proteins. In summary, human engineered cell lines stably expressing large ECM proteins such as human collagen I/II and fibronectin were successfully prepared, and secretion of the ECM components into the surrounding environment was confirmed by immunocytochemistry. Thus, human ECM engineered cells naturally secreting ECM components could be valuable for studying the relationship between the native ECM microenvironment and stem cell behavior.

  13. Intuitive engineering, human factors, and the design of future interfaces (Invited Paper)

    Science.gov (United States)

    Sampson, James B.

    2005-05-01

    Human factors engineering (HFE) professionals complain that they are often called in after-the-fact to help correct human interface problems. They believe many design flaws can be avoided if design teams involve them early on. However, in the case of innovative technology, such post hoc human factors may not be avoidable unless the inventor is also a human factors engineer or the prospective user. In rare cases an inventor of a new technology has an intuitive understanding of human engineering principles and knows well the capabilities and limitations of operators. This paper outlines the importance of focusing on the user-system interface and encouraging engineers to develop their own intuitive sense of users through mental imagery. If design engineers start with a clear mental picture of a specific user and task rather than generalities of use, fewer interface problems are likely to be encountered later in development. Successful technology innovators often use a visual thinking approach in the development of new concepts. Examples are presented to illustrate the successful application of intuitive design. An approach is offered on how designers can improve their non-verbal thinking skills. The author shares the view that the mission of HFE should not be to make system developers dependent on the small community of HF experts but rather to help them learn the value of applying user-centered design techniques.

  14. Human flexor tendon tissue engineering: decellularization of human flexor tendons reduces immunogenicity in vivo.

    Science.gov (United States)

    Raghavan, Shyam S; Woon, Colin Y L; Kraus, Armin; Megerle, Kai; Choi, Matthew S S; Pridgen, Brian C; Pham, Hung; Chang, James

    2012-04-01

    In mutilating hand injuries, tissue engineered tendon grafts may provide a reconstructive solution. We have previously described a method to decellularize cadaveric human flexor tendons while preserving mechanical properties and biocompatibility. The purpose of this study is to evaluate the immunogenicity and strength of these grafts when implanted into an immunocompetent rat model. Cadaveric human flexor tendons were divided into two groups. Group 1 was untreated, and Group 2 was decellularized by treatment with sodium dodecyl sulfate (SDS), ethylenediaminetetraacetic acid (EDTA), and peracetic acid (PAA). Both groups were then analyzed for the presence of major histocompatibility complexes by immunohistochemistry (IHC). Pair-matched tendons from each group were then placed into the dorsal subcutaneous tissue and anchored to the spinal ligaments of Wistar rats for 2 or 4 weeks, and harvested. The infiltration of B-cells and macrophages was determined using IHC. The explants where then subjected to mechanical testing to determine the ultimate tensile stress (UTS) and elastic modulus (EM). Statistical analysis was performed using a paired Student's t-test. The decellularization protocol successfully removed cells and MHC-1 complexes. At 2 weeks after implantation, there was increased infiltration of B-cells in Group 1 (untreated) compared with Group 2 (acellular), both in the capsule and tendon substance. There was improved ultimate tensile stress (UTS, 42.7 ± 8.3 vs. 22.8 ± 7.8 MPa, ptendons that were decellularized. At 4 weeks, there was continued B-cell infiltration in Group 1 (untreated) compared with Group 2 (acellular). There was no appreciable difference in macrophage infiltration at both time points. At 4 weeks Group 2 (acellular) demonstrated persistently greater UTS (40.5 ± 9.1 vs. 14.6 ± 4.2 MPa, ptendons that were decellularized with SDS, EDTA, and PAA resulted in removal of cellular antigens and a decreased immune response when placed into Wistar

  15. Orienteering: An Annotated Bibliography = Orientierungslauf: Eine kommentierte Bibliographie.

    Science.gov (United States)

    Seiler, Roland, Ed.; Hartmann, Wolfgang, Ed.

    1994-01-01

    Annotated bibliography of 220 books, monographs, and journal articles on orienteering published 1984-94, from SPOLIT database of the Federal Institute of Sport Science (Cologne, Germany). Annotations in English or German. Ten sections including psychological, physiological, health, sociological, and environmental aspects; training and coaching;…

  16. Teacher Evaluation: An Annotated Bibliography. Current Bibliography No. 8.

    Science.gov (United States)

    Blount, Gail, Comp.

    Three interrelated frames of reference -- the purposes of evaluation, the criteria for evaluation, and the evaluators -- form the basis of the major subject divisions of this bibliography. Part one consists of general material on teacher evaluation, teacher effectiveness, and supervisory practices. Attempts to determine the qualities of a good…

  17. Le bilinguisme chez les enfants: bibliographie (Child Bilingualism: a Bibliography)

    Science.gov (United States)

    Lundgren-Turiaf, Simone

    1976-01-01

    This bibliography on child bilingualism in France contains 82 entries distributed among three sections: 1) psychosociologic and cultural aspects of migrant worker children, learning conditions; 2) bilingual-bicultural education, experiences and methodological problems; and 3) bilingualism in the countries of origin. (Text is in French.) (TL)

  18. The Open School. An Annotated Bibliography. Current Bibliography No. 4.

    Science.gov (United States)

    Cockburn, Ilze, Comp.

    In 1970, the OISE library published an annotated bibliography entitled, Open Plan (See ED 051 549), that covered the design of open plan schools and the educational practices connected with these facilities. Since then, a clearer distinction has developed between the terms "open plan" and "open education." This revision of the earlier volume…

  19. A Bibliography of Bibliographies of Tests and Assessment Instruments.

    Science.gov (United States)

    Holmes, Jill M.; And Others

    1979-01-01

    Lists bibliographies under headings of adult basic education, arts, attitudes, bilingual students, drugs/tobacco, early childhood, educational assessment, environmental education, general, gifted, handicapped, health/physical education, intelligence, interests, language arts/reading, mathematics, music, organizational management, personality,…

  20. A bibliography of literature pertaining to plague (Yersinia pestis)

    Science.gov (United States)

    Ellison, Laura E.; Frank, Megan K. Eberhardt

    2011-01-01

    Plague is an acute and often fatal zoonotic disease caused by the bacterium Yersinia pestis. Y. pestis mainly cycles between small mammals and their fleas; however, it has the potential to infect humans and frequently causes fatalities if left untreated. It is often considered a disease of the past; however, since the late 1800s, plagueis geographic range has expanded greatly, posing new threats in previously unaffected regions of the world, including the Western United States. A literature search was conducted using Internet resources and databases. The keywords chosen for the searches included plague, Yersinia pestis, management, control, wildlife, prairie dogs, fleas, North America, and mammals. Keywords were used alone or in combination with the other terms. Although this search pertains mostly to North America, citations were included from the international research community, as well. Databases and search engines used included Google (http://www.google.com), Google Scholar (http://scholar.google.com), SciVerse Scopus (http://www.scopus.com), ISI Web of Knowledge (http://apps.isiknowledge.com), and the USGS Library's Digital Desktop (http://library.usgs.gov). The literature-cited sections of manuscripts obtained from keyword searches were cross-referenced to identify additional citations or gray literature that was missed by the Internet search engines. This Open-File Report, published as an Internet-accessible bibliography, is intended to be periodically updated with new citations or older references that may have been missed during this compilation. Hence, the authors would be grateful to receive notice of any new or old papers that the audience (users) think need to be included.

  1. DOE Hydropower Program biennial report 1990--1991 (with updated annotated bibliography)

    Energy Technology Data Exchange (ETDEWEB)

    Chappell, J.R.; Rinehart, B.N.; Sommers, G.L. (Idaho National Engineering Lab., Idaho Falls, ID (United States)); Sale, M.J. (Oak Ridge National Lab., TN (United States))

    1991-07-01

    This report summarizes the activities of the US Department of Energy's (DOE) Hydropower Program for fiscal years 1990 and 1991, and provides an annotated bibliography of research, engineering, operations, regulations, and costs of projects pertinent to hydropower development. The Hydropower Program is organized as follows: background (including Technology Development and Engineering Research and Development); Resource Assessment; National Energy Strategy; Technology Transfer; Environmental Research; and, the bibliography discusses reports written by both private and non-Federal Government sectors. Most reports are available from the National Technical Information Service. 5 figs., 2 tabs.

  2. Integrating the human element into the systems engineering process and MBSE methodology.

    Energy Technology Data Exchange (ETDEWEB)

    Tadros, Michael Samir.

    2013-12-01

    In response to the challenges related to the increasing size and complexity of systems, organizations have recognized the need to integrate human considerations in the beginning stages of systems development. Human Systems Integration (HSI) seeks to accomplish this objective by incorporating human factors within systems engineering (SE) processes and methodologies, which is the focus of this paper. A representative set of HSI methods from multiple sources are organized, analyzed, and mapped to the systems engineering Vee-model. These methods are then consolidated and evaluated against the SE process and Models-Based Systems Engineering (MBSE) methodology to determine where and how they could integrate within systems development activities in the form of specific enhancements. Overall conclusions based on these evaluations are presented and future research areas are proposed.

  3. Effects of mechanical loading on human mesenchymal stem cells for cartilage tissue engineering.

    Science.gov (United States)

    Choi, Jane Ru; Yong, Kar Wey; Choi, Jean Yu

    2017-05-19

    Today, articular cartilage damage is a major health problem, affecting people of all ages. The existing conventional articular cartilage repair techniques, such as autologous chondrocyte implantation (ACI), microfracture, and mosaicplasty, have many shortcomings which negatively affect their clinical outcomes. Therefore, it is essential to develop an alternative and efficient articular repair technique that can address those shortcomings. Cartilage tissue engineering, which aims to create a tissue-engineered cartilage derived from human mesenchymal stem cells (MSCs), shows great promise for improving articular cartilage defect therapy. However, the use of tissue-engineered cartilage for the clinical therapy of articular cartilage defect still remains challenging. Despite the importance of mechanical loading to create a functional cartilage has been well demonstrated, the specific type of mechanical loading and its optimal loading regime is still under investigation. This review summarizes the most recent advances in the effects of mechanical loading on human MSCs. First, the existing conventional articular repair techniques and their shortcomings are highlighted. The important parameters for the evaluation of the tissue-engineered cartilage, including chondrogenic and hypertrophic differentiation of human MSCs are briefly discussed. The influence of mechanical loading on human MSCs is subsequently reviewed and the possible mechanotransduction signaling is highlighted. The development of non-hypertrophic chondrogenesis in response to the changing mechanical microenvironment will aid in the establishment of a tissue-engineered cartilage for efficient articular cartilage repair. © 2017 Wiley Periodicals, Inc.

  4. Survey Result of the Engineering Undergraduate Student's “Human Performance”

    Science.gov (United States)

    Nakayama, Minoru; Takahashi, Hideaki; Kusakabe, Osamu; Ohtaguchi, Kazuhisa; Mizutani, Nobuyasu

    Development of engineer's “Human Performance” is being required to respond to various changes. “Human Performace” is defined as an ability of putting own knowledge and skill to a practical issue. Current engineering undergraduate education promotes to learn this ability. To examine effectiveness of the educational program, a questionnaire consisting of 66 items was developed and the survey was conducted across eight universities. As results, most students recognize importance of the ability, but their achievement is lower for English communication skill and adaptation of cultural difference. They learned the ability on laboratory experience for their thesis, experiment class, club activities, part-time jobs and other activities.

  5. Human Cardiac Tissue Engineering: From Pluripotent Stem Cells to Heart Repair

    Science.gov (United States)

    Jackman, Christopher P.; Shadrin, Ilya Y.; Carlson, Aaron L.; Bursac, Nenad

    2014-01-01

    Engineered cardiac tissues hold great promise for use in drug and toxicology screening, in vitro studies of human physiology and disease, and as transplantable tissue grafts for myocardial repair. In this review, we discuss recent progress in cell-based therapy and functional tissue engineering using pluripotent stem cell-derived cardiomyocytes and we describe methods for delivery of cells into the injured heart. While significant hurdles remain, notable advances have been made in the methods to derive large numbers of pure human cardiomyocytes, mature their phenotype, and produce and implant functional cardiac tissues, bringing the field a step closer to widespread in vitro and in vivo applications. PMID:25599018

  6. A Virtual Campus Based on Human Factor Engineering

    Science.gov (United States)

    Yang, Yuting; Kang, Houliang

    2014-01-01

    Three Dimensional or 3D virtual reality has become increasingly popular in many areas, especially in building a digital campus. This paper introduces a virtual campus, which is based on a 3D model of The Tourism and Culture College of Yunnan University (TCYU). Production of the virtual campus was aided by Human Factor and Ergonomics (HF&E), an…

  7. A Virtual Campus Based on Human Factor Engineering

    Science.gov (United States)

    Yang, Yuting; Kang, Houliang

    2014-01-01

    Three Dimensional or 3D virtual reality has become increasingly popular in many areas, especially in building a digital campus. This paper introduces a virtual campus, which is based on a 3D model of The Tourism and Culture College of Yunnan University (TCYU). Production of the virtual campus was aided by Human Factor and Ergonomics (HF&E), an…

  8. Engineering Data Compendium. Human Perception and Performance. Volume 3

    Science.gov (United States)

    1988-01-01

    Breitmeyer, B. G. (1975). Sim- cies to sinusoidal gratings. Vision human observers: A probabilistic pie reaction time as a measure of Research. 16...oscilloscope; viewed at optical infinity through a plano - convex lens (153 mm diameter, 340 mm focal length) one focal length from display • Patch wom

  9. Bibliography on Decline and Retrenchment.

    Science.gov (United States)

    National Center for Higher Education Management Systems, Boulder, CO.

    A bibliography on decline and retrenchment in higher education is presented that includes publications from the fields of higher education, the organization sciences, and public administration. The objective is to make available the reference tools that have been useful in conducting the National Center for Higher Education Management Systems'…

  10. Child Development: An Annotated Bibliography.

    Science.gov (United States)

    Dickerson, LaVerne Thornton, Comp.

    This annotated bibliography focuses on recent publications dealing with factors that influence child growth and development, rather than the developmental processes themselves. Topics include: general sources on child development; physical and perceptual-motor development; cognitive development; social and personality development; and play.…

  11. Children and Advertising: A Bibliography.

    Science.gov (United States)

    Council of Better Business Bureaus, Inc., New York, NY. Clearinghouse for Research on Children's Advertising.

    More than 600 entries are contained in this annotated bibliography of materials concerning the effects of television advertising on children. Entries are alphabetized by author and are divided into the following categories: special interest articles; general interest articles; books and pamphlets; government publications; transcripts of oral…

  12. Strategic Management: A Comprehensive Bibliography.

    Science.gov (United States)

    Chaffee, Ellen Earle; de Alba, Renee

    A bibliography on strategic management is presented to assist both practitioners and researchers. Criteria for inclusion were as follows: (1) general in scope, providing introductory information on a variety of subtopics within strategic management; (2) indications that the work is becoming a classic (i.e., frequent citations by other authors);…

  13. TQM - Total Quality Management (Bibliography)

    Science.gov (United States)

    1990-05-01

    TOTAL QUALITY MANAGEMENT (BIBLIOORAPHY) PREPARED BY... Total Quality Management (TQM), it is an appropriate time to provide an overview of the literature available on the topic. The Total Quality Management bibliography...Secretary 32 CFR Part 281 Total Quality Management Agency: Office of the Secretary, DOD Action: Proposed rule Summary: This proposed rule establishes

  14. Bibliographies for Research on Women

    Science.gov (United States)

    Ballou, Patricia K.

    1977-01-01

    Some of the more significant bibliographic works that have appeared since 1970 are discussed. Bibliographies are given for the following areas: history, literature, the arts, philosophy, religion, anthropology and area studies, economics and employment, professions, education, politics and law, sociology, psychology, and health. (AM)

  15. Hyperactivity: Exceptional Child Bibliography Series.

    Science.gov (United States)

    Council for Exceptional Children, Reston, VA. Information Center on Exceptional Children.

    Containing 27 references on hyperactivity selected from "Exceptional Child Education Abstracts", the bibliography cites research reports, conference papers, journal articles, texts, and program guides. Each entry provides bibliographical data, availability information, indexing and retrieval descriptors, and abstracts of the documents. The…

  16. Autism: Exceptional Child Bibliography Series.

    Science.gov (United States)

    Council for Exceptional Children, Reston, VA. Information Center on Exceptional Children.

    One in a series of over 50 similar selected listings, the bibliography contains 47 items of research reports, conference papers, journal articles, texts, and program guides selected from "Exceptional Child Education Abstracts". Each entry on autism provides bibliographical data, availability information, indexing and retrieval descriptors, and…

  17. Teacher Evaluation: An Annotated Bibliography.

    Science.gov (United States)

    McKenna, Bernard H.; And Others

    In his introduction to the 86-item annotated bibliography by Mueller and Poliakoff, McKenna discusses his views on teacher evaluation and his impressions of the documents cited. He observes, in part, that the current concern is with the process of evaluation and that most researchers continue to believe that student achievement is the most…

  18. Agriculture & Agronomy: A Dissertation Bibliography.

    Science.gov (United States)

    1978

    This bibliography presents a compilation of Agriculture and Agronomy doctoral research for the years 1973-1976. Each of the 3,386 doctoral dissertations cited herein has been accepted by accredited degree-granting universities in North America and published by University Microfilms International (UMI). Dissertations are arranged alphabetically in…

  19. Meaningful Assessment: An Annotated Bibliography.

    Science.gov (United States)

    Thrond, Mary A.

    The annotated bibliography contains citations of nine references on alternative student assessment methods in second language programs, particularly at the secondary school level. The references include a critique of conventional reading comprehension assessment, a discussion of performance assessment, a proposal for a multi-trait, multi-method…

  20. Bibliography for the Hospitality Industry.

    Science.gov (United States)

    Nelson, Elizabeth A.

    This annotated bibliography is a sample collection of reference materials in the hospitality industry suitable for a small academic library. It is assumed that the library has a general reference collection. Publication dates range from 1992-96, with two publication dates in the 1980s. No periodicals are included. The 41 reference materials are…

  1. Wind energy utilization: A bibliography

    Science.gov (United States)

    1975-01-01

    Bibliography cites documents published to and including 1974 with abstracts and references, and is indexed by topic, author, organization, title, and keywords. Topics include: Wind Energy Potential and Economic Feasibility, Utilization, Wind Power Plants and Generators, Wind Machines, Wind Data and Properties, Energy Storage, and related topics.

  2. Black Holes: A Selected Bibliography.

    Science.gov (United States)

    Fraknoi, Andrew

    1991-01-01

    Offers a selected bibliography pertaining to black holes with the following categories: introductory books; introductory articles; somewhat more advanced articles; readings about Einstein's general theory of relativity; books on the death of stars; articles on the death of stars; specific articles about Supernova 1987A; relevant science fiction…

  3. Bibliographies for Research on Women

    Science.gov (United States)

    Ballou, Patricia K.

    1977-01-01

    Some of the more significant bibliographic works that have appeared since 1970 are discussed. Bibliographies are given for the following areas: history, literature, the arts, philosophy, religion, anthropology and area studies, economics and employment, professions, education, politics and law, sociology, psychology, and health. (AM)

  4. Annotated Bibliography on Humanistic Education

    Science.gov (United States)

    Ganung, Cynthia

    1975-01-01

    Part I of this annotated bibliography deals with books and articles on such topics as achievement motivation, process education, transactional analysis, discipline without punishment, role-playing, interpersonal skills, self-acceptance, moral education, self-awareness, values clarification, and non-verbal communication. Part II focuses on…

  5. Nikos Kazantzakis: An Annotated Bibliography.

    Science.gov (United States)

    Qiu, Kui

    This research paper consists of an annotated bibliography about Nikos Kazantzakis, one of the major modern Greek writers and author of "The Last Temptation of Christ,""Zorba the Greek," and many other works. Because of Kazantzakis' position in world literature there are many critical works about him; however, bibliographical control of these works…

  6. Engineering targeted chromosomal amplifications in human breast epithelial cells.

    Science.gov (United States)

    Springer, Simeon; Yi, Kyung H; Park, Jeenah; Rajpurohit, Anandita; Price, Amanda J; Lauring, Josh

    2015-07-01

    Chromosomal amplifications are among the most common genetic alterations found in human cancers. However, experimental systems to study the processes that lead to specific, recurrent amplification events in human cancers are lacking. Moreover, some common amplifications, such as that at 8p11-12 in breast cancer, harbor multiple driver oncogenes, which are poorly modeled by conventional overexpression approaches. We sought to develop an experimental system to model recurrent chromosomal amplification events in human cell lines. Our strategy is to use homologous-recombination-mediated gene targeting to deliver a dominantly selectable, amplifiable marker to a specified chromosomal location. We used adeno-associated virus vectors to target human MCF-7 breast cancer cells at the ZNF703 locus, in the recurrent 8p11-12 amplicon, using the E. coli inosine monophosphate dehydrogenase (IMPDH) enzyme as a marker. We applied selective pressure using IMPDH inhibitors. Surviving clones were found to have increased copy number of ZNF703 (average 2.5-fold increase) by droplet digital PCR and FISH. Genome-wide array comparative genomic hybridization confirmed that amplifications had occurred on the short arm of chromosome 8, without changes on 8q or other chromosomes. Patterns of amplification were variable and similar to those seen in primary human breast cancers, including "sawtooth" patterns, distal copy number loss, and large continuous regions of copy number gain. This system will allow study of the cis- and trans-acting factors that are permissive for chromosomal amplification and provide a model to analyze oncogene cooperativity in amplifications harboring multiple candidate driver genes.

  7. A Domain Analysis Bibliography

    Science.gov (United States)

    1990-06-01

    Bauhaus , a prototype CASE workstation for D-SAPS development. [ARAN88A] Guillermo F. Arango. Domain Engineering for Software Reuse. PhD thesis...34 VITA90B: Domain Analysis within the ISEC Rapid Center 48 CMU/SEI-90-SR-3 Appendix III Alphabetical by Organization/Project BAUHAUS * ALLE87A

  8. A comparative analysis of reading strategies across ESP students of humanities and engineering

    Directory of Open Access Journals (Sweden)

    Mehdi Vaez Dalili

    2013-04-01

    Full Text Available This study set out to explore whether significant differences existed between two groups of EFL students of different fields of study (i.e. humanities and engineering in terms of their metacognitive awareness and use of certain reading strategies while reading English for Specific Purposes (ESP texts. Seventy lower-intermediate college-level students (35 engineering students and 35 students of humanities completed the Metacognitive Awareness of Reading Strategies Inventory (Mokhtari & Reichard, 2002 to measure their metacognitive awareness of ESP reading strategies. The inventory includes 3 strategy subscales with 30 items: Global Reading Strategies (GLOB, Problem-Solving Strategies (PROB, and Support Reading Strategies (SUP. A series of t-tests were conducted to figure out the differences between the two groups of students in connection with each of the strategies in the MARSI inventory. The results showed that in spite of the fact that the two groups were studying different fields of study, they reported strikingly similar patterns of reading strategy awareness and confirmed having used almost all of the strategies while reading ESP materials. However, the engineering students reported using some types of reading strategies more frequently than did the humanities students. The findings of the study will not only explain the differences and similarities between engineering and humanities students, but also help to challenge the purely speculative assumption as to the deficiencies in strategy-based ESP reading comprehension of humanities students.

  9. Engineered human broncho-epithelial tissue-like assemblies

    Science.gov (United States)

    Goodwin, Thomas J. (Inventor)

    2012-01-01

    Three-dimensional human broncho-epithelial tissue-like assemblies (TLAs) are produced in a rotating wall vessel (RWV) with microcarriers by coculturing mesenchymal bronchial-tracheal cells (BTC) and bronchial epithelium cells (BEC). These TLAs display structural characteristics and express markers of in vivo respiratory epithelia. TLAs are useful for screening compounds active in lung tissues such as antiviral compounds, cystic fibrosis treatments, allergens, and cytotoxic compounds.

  10. Engineering Research Division report on reports: calendar year 1979. [LLL

    Energy Technology Data Exchange (ETDEWEB)

    Gardner, C.L.; Johnston, S.J. (eds.)

    1980-03-01

    A bibliography of publications of members of the Engineering Research Division of the Electronics Engineering Department is presented for 1979. Abstracts for 148 publications are included, along with author and keywork indexes. (RWR)

  11. Tissue engineering potential of human dermis-isolated adult stem cells from multiple anatomical locations.

    Science.gov (United States)

    Kwon, Heenam; Haudenschild, Anne K; Brown, Wendy E; Vapniarsky, Natalia; Paschos, Nikolaos K; Arzi, Boaz; Hu, Jerry C; Athanasiou, Kyriacos A

    2017-01-01

    Abundance and accessibility render skin-derived stem cells an attractive cell source for tissue engineering applications. Toward assessing their utility, the variability of constructs engineered from human dermis-isolated adult stem (hDIAS) cells was examined with respect to different anatomical locations (foreskin, breast, and abdominal skin), both in vitro and in a subcutaneous, athymic mouse model. All anatomical locations yielded hDIAS cells with multi-lineage differentiation potentials, though adipogenesis was not seen for foreskin-derived hDIAS cells. Using engineered cartilage as a model, tissue engineered constructs from hDIAS cells were compared. Construct morphology differed by location. The mechanical properties of human foreskin- and abdominal skin-derived constructs were similar at implantation, remaining comparable after 4 additional weeks of culture in vivo. Breast skin-derived constructs were not mechanically testable. For all groups, no signs of abnormality were observed in the host. Addition of aggregate redifferentiation culture prior to construct formation improved chondrogenic differentiation of foreskin-derived hDIAS cells, as evident by increases in glycosaminoglycan and collagen contents. More robust Alcian blue staining and homogeneous cell populations were also observed compared to controls. Human DIAS cells elicited no adverse host responses, reacted positively to chondrogenic regimens, and possessed multi-lineage differentiation potential with the caveat that efficacy may differ by anatomical origin of the skin. Taken together, these results suggest that hDIAS cells hold promise as a potential cell source for a number of tissue engineering applications.

  12. The experimental study of genetic engineering human neural stem cells mediated by lentivirus to express multigene

    Institute of Scientific and Technical Information of China (English)

    CAI Pei-qiang; TANG Xun; LIN Yue-qiu; Oudega Martin; SUN Guang-yun; XU Lin; YANG Yun-kang; ZHOU Tian-hua

    2006-01-01

    Objective:To explore the feasibility to construct genetic engineering human neural stem cells (hNSCs)mediated by lentivirus to express multigene in order to provide a graft source for further studies of spinal cord injury (SCI).Methods: Human neural stem cells from the brain cortex of human abortus were isolated and cultured, then gene was modified by lentivirus to express both green fluorescence protein (GFP) and rat neurotrophin-3(NT-3); the transgenic expression was detected by the methods of fluorescence microscope, dorsal root ganglion of fetal rats and slot blot.Results: Genetic engineering hNSCs were successfully constructed. All of the genetic engineering hNSCs which expressed bright green fluorescence were observed under the fluorescence microscope. The conditioned medium of transgenic hNSCs could induce neurite flourishing outgrowth from dorsal root ganglion (DRG). The genetic engineering hNSCs expressed high level NT-3 which could be detected by using slot blot.Conclusions: Genetic engineering hNSCs mediated by lentivirus can be constructed to express multigene successfully.

  13. Optimal Configuration of Human Motion Tracking Systems: A Systems Engineering Approach

    Science.gov (United States)

    Henderson, Steve

    2005-01-01

    Human motion tracking systems represent a crucial technology in the area of modeling and simulation. These systems, which allow engineers to capture human motion for study or replication in virtual environments, have broad applications in several research disciplines including human engineering, robotics, and psychology. These systems are based on several sensing paradigms, including electro-magnetic, infrared, and visual recognition. Each of these paradigms requires specialized environments and hardware configurations to optimize performance of the human motion tracking system. Ideally, these systems are used in a laboratory or other facility that was designed to accommodate the particular sensing technology. For example, electromagnetic systems are highly vulnerable to interference from metallic objects, and should be used in a specialized lab free of metal components.

  14. Developing Novel Therapeutic Approaches in Small Cell Lung Carcinoma Using Genetically Engineered Mouse Models and Human Circulating Tumor Cells

    Science.gov (United States)

    2015-10-01

    Using Genetically Engineered Mouse Models and Human Circulating Tumor Cells PRINCIPAL INVESTIGATOR: Jeffrey Engelman MD PhD CONTRACTING...SUBTITLE Developiing Novel Therapeutic Approaches in Small Cell Lung 5a. CONTRACT NUMBER Carcinoma Using Genetically Engineered Mouse Models and 5b...biomarkers. 15. SUBJECT TERMS Small cell lung cancer (SCLC), Genetically engineered mouse model (GEMM), BH3 mimetic, TORC inhibitor, Apoptosis

  15. Human Systems Engineering for Launch processing at Kennedy Space Center (KSC)

    Science.gov (United States)

    Henderson, Gena; Stambolian, Damon B.; Stelges, Katrine

    2012-01-01

    Launch processing at Kennedy Space Center (KSC) is primarily accomplished by human users of expensive and specialized equipment. In order to reduce the likelihood of human error, to reduce personal injuries, damage to hardware, and loss of mission the design process for the hardware needs to include the human's relationship with the hardware. Just as there is electrical, mechanical, and fluids, the human aspect is just as important. The focus of this presentation is to illustrate how KSC accomplishes the inclusion of the human aspect in the design using human centered hardware modeling and engineering. The presentations also explain the current and future plans for research and development for improving our human factors analysis tools and processes.

  16. Human amniotic fluid derived cells can competently substitute dermal fibroblasts in a tissue-engineered dermo-epidermal skin analog

    NARCIS (Netherlands)

    Hartmann-Fritsch, Fabienne; Hosper, Nynke; Luginbuehl, Joachim; Biedermann, Thomas; Reichmann, Ernst; Meuli, Martin

    Human amniotic fluid comprises cells with high differentiation capacity, thus representing a potential cell source for skin tissue engineering. In this experimental study, we investigated the ability of human amniotic fluid derived cells to substitute dermal fibroblasts and support epidermis

  17. Draft audit report, human factors engineering control room design review: Saint Lucie Nuclear Power Plant, Unit No. 2

    Energy Technology Data Exchange (ETDEWEB)

    Peterson, L.R.; Lappa, D.A.; Moore, J.W.

    1981-09-03

    A human factors engineering preliminary design review of the Saint Lucie Unit 2 control room was performed at the site on August 3 through August 7, 1981. This design review was carried out by a team from the Human Factors Engineering Branch, Division of Human Factors Safety. This report was prepared on the basis of the HFEB's review of the applicant's Preliminary Design Assessment and the human factors engineering design review/audit performed at the site. The review team included human factors consultants from BioTechnology, Inc., Falls Church, Virginia, and from Lawrence Livermore National Laboratory (University of California), Livermore, California.

  18. Normal human epithelial cells regulate the size and morphology of tissue-engineered capillaries.

    Science.gov (United States)

    Rochon, Marie-Hélène; Fradette, Julie; Fortin, Véronique; Tomasetig, Florence; Roberge, Charles J; Baker, Kathleen; Berthod, François; Auger, François A; Germain, Lucie

    2010-05-01

    The survival of thick tissues/organs produced by tissue engineering requires rapid revascularization after grafting. Although capillary-like structures have been reconstituted in some engineered tissues, little is known about the interaction between normal epithelial cells and endothelial cells involved in the in vitro angiogenic process. In the present study, we used the self-assembly approach of tissue engineering to examine this relationship. An endothelialized tissue-engineered dermal substitute was produced by adding endothelial cells to the tissue-engineered dermal substitute produced by the self-assembly approach. The latter consists in culturing fibroblasts in the medium supplemented with serum and ascorbic acid. A network of tissue-engineered capillaries (TECs) formed within the human extracellular matrix produced by dermal fibroblasts. To determine whether epithelial cells modify TECs, the size and form of TECs were studied in the endothelialized tissue-engineered dermal substitute cultured in the presence or absence of epithelial cells. In the presence of normal keratinocytes from skin, cornea or uterine cervix, endothelial cells formed small TECs (cross-sectional area estimated at less than 50 microm(2)) reminiscent of capillaries found in the skin's microcirculation. In contrast, TECs grown in the absence of epithelial cells presented variable sizes (larger than 50 microm(2)), but the addition of keratinocyte-conditioned media or exogenous vascular endothelial growth factor induced their normalization toward a smaller size. Vascular endothelial growth factor neutralization inhibited the effect of keratinocyte-conditioned media. These results provide new direct evidence that normal human epithelial cells play a role in the regulation of the underlying TEC network, and advance our knowledge in tissue engineering for the production of TEC networks in vitro.

  19. Sequential use of human-derived medium supplements favours cardiovascular tissue engineering

    NARCIS (Netherlands)

    Vis, Paul W. Riem; Sluijter, Joost P. G.; Soekhradj-Soechit, R. Sarita; van Herwerden, Lex A.; Kluin, Jolanda; Bouten, Carlijn V. C.

    2012-01-01

    For clinical application of tissue engineering strategies, the use of animal-derived serum in culture medium is not recommended, because it can evoke immune responses in patients. We previously observed that human platelet-lysate (PL) is favourable for cell expansion, but generates weaker tissue as

  20. Sequential use of human-derived medium supplements favours cardiovascular tissue engineering

    NARCIS (Netherlands)

    Vis, Paul W. Riem; Sluijter, Joost P. G.; Soekhradj-Soechit, R. Sarita; van Herwerden, Lex A.; Kluin, Jolanda; Bouten, Carlijn V. C.

    2012-01-01

    For clinical application of tissue engineering strategies, the use of animal-derived serum in culture medium is not recommended, because it can evoke immune responses in patients. We previously observed that human platelet-lysate (PL) is favourable for cell expansion, but generates weaker tissue as

  1. A search engine for retrieval and inspection of events with 48 human actions in realistic videos

    NARCIS (Netherlands)

    Burghouts, G.J.; Penning, H.L.H. de; Hove, R.J.M. ten; Landsmeer, S.; Broek, S.P. van den; Hollander, R.J.M.; Hanckmann, P.; Kruithof, M.C.; Leeuwen, C.J. van; Korzec, S.; Bouma, H.; Schutte, K.

    2013-01-01

    The contribution of this paper is a search engine that recognizes and describes 48 human actions in realistic videos. The core algorithms have been published recently, from the early visual processing (Bouma, 2012), discriminative recognition (Burghouts, 2012) and textual description (Hankmann, 2012

  2. Human factors engineering for the TERF (Tritium Emissions Reduction Facility) project. [Tritium Emissions Reduction Facility

    Energy Technology Data Exchange (ETDEWEB)

    Hedley, W.H.; Adams, F.S. (EG and G Mound Applied Technologies, Miamisburg, OH (USA)); Wells, J.E. (Lawrence Livermore National Lab., CA (USA))

    1990-12-14

    The Tritium Emissions Reduction Facility (TERF) is being built by EG G Mound Applied Technologies to provide improved control of the tritium emissions from gas streams being processed. Mound handles tritium in connection with production, development, research, disassembly, recovery, and surveillance operations. During these operations, a small fraction of the tritium being processed escapes from its original containment. The objective of this report is to describe the human factors engineering as performed in connection with the design, construction, and testing of the TERF as required in DOE Order 6430.1A, section 1300-12. Human factors engineering has been involved at each step of the process and was considered during the preliminary research on tritium capture before selecting the specific process to be used. Human factors engineering was also considered in determining the requirements for the TERF and when the specific design work was initiated on the facility and the process equipment. Finally, human factors engineering was used to plan the specific acceptance tests that will be made during TERF installation and after its completion. These tests will verify the acceptability of the final system and its components. 16 refs., 8 figs.

  3. Comparative DNA damage and transcriptomic effects of engineered nanoparticles in human lung cells in vitro

    Science.gov (United States)

    A series of six titanium dioxide and two cerium oxide engineered nanomaterials were assessed for their ability to induce cytotoxicity, reactive oxygen species (ROS), various types of DNA damage, and transcriptional changes in human respiratory BEAS-2B cells exposed in vitro at se...

  4. Ergonomics and human factors: the paradigms for science, engineering, design, technology and management of human-compatible systems.

    Science.gov (United States)

    Karwowski, W

    2005-04-15

    This paper provides a theoretical perspective on human factors and ergonomics (HFE), defined as a unique and independent discipline that focuses on the nature of human-artefact interactions, viewed from the unified perspective of the science, engineering, design, technology and management of human-compatible systems. Such systems include a variety of natural and artificial products, processes and living environments. The distinguishing features of the contemporary HFE discipline and profession are discussed and a concept of ergonomics literacy is proposed. An axiomatic approach to ergonomics design and a universal measure of system-human incompatibility are also introduced. It is concluded that the main focus of the HFE discipline in the 21st century will be the design and management of systems that satisfy human compatibility requirements.

  5. Genetic engineering of human embryonic stem cells with lentiviral vectors.

    Science.gov (United States)

    Xiong, Chen; Tang, Dong-Qi; Xie, Chang-Qing; Zhang, Li; Xu, Ke-Feng; Thompson, Winston E; Chou, Wayne; Gibbons, Gary H; Chang, Lung-Ji; Yang, Li-Jun; Chen, Yuqing E

    2005-08-01

    Human embryonic stem (hES) cells present a valuable source of cells with a vast therapeutic potential. However, the low efficiency of directed differentiation of hES cells remains a major obstacle in their uses for regenerative medicine. While differentiation may be controlled by the genetic manipulation, effective and efficient gene transfer into hES cells has been an elusive goal. Here, we show stable and efficient genetic manipulations of hES cells using lentiviral vectors. This method resulted in the establishment of stable gene expression without loss of pluripotency in hES cells. In addition, lentiviral vectors were effective in conveying the expression of an U6 promoter-driven small interfering RNA (siRNA), which was effective in silencing its specific target. Taken together, our results suggest that lentiviral gene delivery holds great promise for hES cell research and application.

  6. What Can Students’ Bibliographies Tell Us? Evidence Based Information Skills

    Directory of Open Access Journals (Sweden)

    Fei Yu

    2006-06-01

    Full Text Available Objective ‐ This project sought to identify students’ strengths and weaknesses in locating, retrieving, and citing information in order to deliver information skills workshops more effectively. Methods ‐ Bibliographies submitted from first‐year engineering and second‐ and fourthyear chemical engineering students’ project reports were analysed for the number of items cited, the variety of items cited, and the correct use of citation style. The topics of the project reports were also reviewed to see the relationships between the topics and the items cited. Results ‐ The results show that upper level students cited more items in total than did lower level students in their bibliographies. Second‐ and fourth‐year engineering students cited more books and journal articles than first‐year students cited. Web sites were used extensively by all three groups of students, and for some first‐year students these were the most frequently used sources. Students from all three groups had difficulties with citation style. Conclusion ‐ There was a clear difference in citation frequency between upper and lower level engineering students. Different strategies of information skills instruction are needed for different levels of students. Librarians and department faculty members need to include good quality Internet resources in their teaching and to change the emphasis from finding information to finding, interpreting, and citing accurately.

  7. Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus.

    Science.gov (United States)

    Muñoz, Inés G; Prieto, Jesús; Subramanian, Sunita; Coloma, Javier; Redondo, Pilar; Villate, Maider; Merino, Nekane; Marenchino, Marco; D'Abramo, Marco; Gervasio, Francesco L; Grizot, Sylvestre; Daboussi, Fayza; Smith, Julianne; Chion-Sotinel, Isabelle; Pâques, Frédéric; Duchateau, Philippe; Alibés, Andreu; Stricher, François; Serrano, Luis; Blanco, Francisco J; Montoya, Guillermo

    2011-01-01

    Homing endonucleases recognize long target DNA sequences generating an accurate double-strand break that promotes gene targeting through homologous recombination. We have modified the homodimeric I-CreI endonuclease through protein engineering to target a specific DNA sequence within the human RAG1 gene. Mutations in RAG1 produce severe combined immunodeficiency (SCID), a monogenic disease leading to defective immune response in the individuals, leaving them vulnerable to infectious diseases. The structures of two engineered heterodimeric variants and one single-chain variant of I-CreI, in complex with a 24-bp oligonucleotide of the human RAG1 gene sequence, show how the DNA binding is achieved through interactions in the major groove. In addition, the introduction of the G19S mutation in the neighborhood of the catalytic site lowers the reaction energy barrier for DNA cleavage without compromising DNA recognition. Gene-targeting experiments in human cell lines show that the designed single-chain molecule preserves its in vivo activity with higher specificity, further enhanced by the G19S mutation. This is the first time that an engineered meganuclease variant targets the human RAG1 locus by stimulating homologous recombination in human cell lines up to 265 bp away from the cleavage site. Our analysis illustrates the key features for à la carte procedure in protein-DNA recognition design, opening new possibilities for SCID patients whose illness can be treated ex vivo.

  8. Acid precipitation; an annotated bibliography

    Science.gov (United States)

    Wiltshire, Denise A.; Evans, Margaret L.

    1984-01-01

    This collection of 1660 bibliographies references on the causes and environmental effects of acidic atmospheric deposition was compiled from computerized literature searches of earth-science and chemistry data bases. Categories of information are (1) atmospheric chemistry (gases and aerosols), (2) precipitation chemistry, (3) transport and deposition (wet and dry), (4) aquatic environments (biological and hydrological), (5) terrestrial environments, (6) effects on materials and structures, (7) air and precipitation monitoring and data collection, and (8) modeling studies. References date from the late 1800 's through December 1981. The bibliography includes short summaries of most documents. Omitted are unpublished manuscripts, publications in press, master 's theses and doctoral dissertations, newspaper articles, and book reviews. Coauthors and subject indexes are included. (USGS)

  9. Bibliography of Doctor Chul Park

    Science.gov (United States)

    Gochberg, Lawrence A.; Venkatapathy, Ethiraj; Park, Chul

    1995-05-01

    This document contains a comprehensive bibliography of the published works, and a short biography, of Dr. Chul Park. The contents of this bibliography were compiled primarily from the NASA RECON data base. The RECON citations have been modified to appear in a uniform format with all other listed citations . These other citations were located by computer searches in the INSPEC, NTIS, COMPENDEX, and Chemical Abstracts data bases, as well as through the cooperation of Dr. Chul Park, and his associates in the Reacting Flow Environments Branch at NASA Ames Research Center. All citations are presented in an approximate reverse chronological order from the present date. This work was created to honor the occasion of Dr. Chul Park's retirement on December 14, 1994, after 27 years of distinguished government service at the NASA Ames Research Center.

  10. Review of actinide-sediment reactions with an annotated bibliography

    Energy Technology Data Exchange (ETDEWEB)

    Ames, L.L.; Rai, D.; Serne, R.J.

    1976-02-10

    The annotated bibliography is divided into sections on chemistry and geochemistry, migration and accumulation, cultural distributions, natural distributions, and bibliographies and annual reviews. (LK)

  11. Annotated geothermal bibliography of Utah

    Energy Technology Data Exchange (ETDEWEB)

    Budding, K.E.; Bugden, M.H. (comps.)

    1986-01-01

    The bibliography includes all the Utah geothermal references through 1984. Some 1985 citations are listed. Geological, geophysical, and tectonic maps and reports are included if they cover a high-temperature thermal area. The references are indexed geographically either under (1) United States (national studies), (2) regional - western United States or physiographic province, (3) Utah - statewide and regional, or (4) county. Reports concerning a particular hot spring or thermal area are listed under both the thermal area and the county names.

  12. Irregular Warfare: A Selected Bibliography

    Science.gov (United States)

    2009-11-01

    Effectiveness: Peace Operations and Beyond. Clementsport: Cana- dian Peacekeeping Press, 2007. 92pp. (JZ5538 .M21 2007) 24 Muse, Robert C...100.2/ADA482274 Lilly , Douglas D. Strategic Framing of Stability Operations. Strategy Research Project. Carlisle Barracks: U.S. Army War College, 20...able in Informaworld 28 OTHER BIBLIOGRAPHIES Armstrong, Glenda, comp. Peace and Stability Operations. Maxwell Air Force Base: Muir S. Fair

  13. Total Quality Management (TQM) Bibliography

    Science.gov (United States)

    1990-04-01

    GTE FIE COPY DTIC c" ECTE 8JUL 25 1990u TOTAL QUALITY MANAGEMENT (TQM) BIBLIOGRAPHY APRIL-1990 Jointly supported by __’__________-_________ Jointly...Arsenal, AL 35898-5241 1I. TITLE (Include Security Classification) TOTAL QUALITY MANAGEMENT (TQM) BIBL IRAPHY APRIL-1990 12. PERSONAL AUTHOR(S) Knott...implementation of the concept of total quality management (TQM). The selected coverage includes books, periodical articles, conference papers and reports. Coded

  14. Tissue-engineered human bioartificial muscles expressing a foreign recombinant protein for gene therapy

    Science.gov (United States)

    Powell, C.; Shansky, J.; Del Tatto, M.; Forman, D. E.; Hennessey, J.; Sullivan, K.; Zielinski, B. A.; Vandenburgh, H. H.

    1999-01-01

    Murine skeletal muscle cells transduced with foreign genes and tissue engineered in vitro into bioartificial muscles (BAMs) are capable of long-term delivery of soluble growth factors when implanted into syngeneic mice (Vandenburgh et al., 1996b). With the goal of developing a therapeutic cell-based protein delivery system for humans, similar genetic tissue-engineering techniques were designed for human skeletal muscle stem cells. Stem cell myoblasts were isolated, cloned, and expanded in vitro from biopsied healthy adult (mean age, 42 +/- 2 years), and elderly congestive heart failure patient (mean age, 76 +/- 1 years) skeletal muscle. Total cell yield varied widely between biopsies (50 to 672 per 100 mg of tissue, N = 10), but was not significantly different between the two patient groups. Percent myoblasts per biopsy (73 +/- 6%), number of myoblast doublings prior to senescence in vitro (37 +/- 2), and myoblast doubling time (27 +/- 1 hr) were also not significantly different between the two patient groups. Fusion kinetics of the myoblasts were similar for the two groups after 20-22 doublings (74 +/- 2% myoblast fusion) when the biopsy samples had been expanded to 1 to 2 billion muscle cells, a number acceptable for human gene therapy use. The myoblasts from the two groups could be equally transduced ex vivo with replication-deficient retroviral expression vectors to secrete 0.5 to 2 microg of a foreign protein (recombinant human growth hormone, rhGH)/10(6) cells/day, and tissue engineered into human BAMs containing parallel arrays of differentiated, postmitotic myofibers. This work suggests that autologous human skeletal myoblasts from a potential patient population can be isolated, genetically modified to secrete foreign proteins, and tissue engineered into implantable living protein secretory devices for therapeutic use.

  15. Tissue-engineered human bioartificial muscles expressing a foreign recombinant protein for gene therapy

    Science.gov (United States)

    Powell, C.; Shansky, J.; Del Tatto, M.; Forman, D. E.; Hennessey, J.; Sullivan, K.; Zielinski, B. A.; Vandenburgh, H. H.

    1999-01-01

    Murine skeletal muscle cells transduced with foreign genes and tissue engineered in vitro into bioartificial muscles (BAMs) are capable of long-term delivery of soluble growth factors when implanted into syngeneic mice (Vandenburgh et al., 1996b). With the goal of developing a therapeutic cell-based protein delivery system for humans, similar genetic tissue-engineering techniques were designed for human skeletal muscle stem cells. Stem cell myoblasts were isolated, cloned, and expanded in vitro from biopsied healthy adult (mean age, 42 +/- 2 years), and elderly congestive heart failure patient (mean age, 76 +/- 1 years) skeletal muscle. Total cell yield varied widely between biopsies (50 to 672 per 100 mg of tissue, N = 10), but was not significantly different between the two patient groups. Percent myoblasts per biopsy (73 +/- 6%), number of myoblast doublings prior to senescence in vitro (37 +/- 2), and myoblast doubling time (27 +/- 1 hr) were also not significantly different between the two patient groups. Fusion kinetics of the myoblasts were similar for the two groups after 20-22 doublings (74 +/- 2% myoblast fusion) when the biopsy samples had been expanded to 1 to 2 billion muscle cells, a number acceptable for human gene therapy use. The myoblasts from the two groups could be equally transduced ex vivo with replication-deficient retroviral expression vectors to secrete 0.5 to 2 microg of a foreign protein (recombinant human growth hormone, rhGH)/10(6) cells/day, and tissue engineered into human BAMs containing parallel arrays of differentiated, postmitotic myofibers. This work suggests that autologous human skeletal myoblasts from a potential patient population can be isolated, genetically modified to secrete foreign proteins, and tissue engineered into implantable living protein secretory devices for therapeutic use.

  16. Bibliography for the Satellite Power System (SPS) Concept Development and Evaluation Program

    Energy Technology Data Exchange (ETDEWEB)

    Abromavage, M.; Calzadilla, R.; Murray, M.

    1981-04-01

    This bibliography encompasses systems definition and engineering aspects; environmental assessment of microwave health and ecology, risks to space workers and atmospheric effects; a societal assessment covering resource requirements (land and materials) international and institutional issues; and a comparative assessment of the SPS Reference System relative to other advanced energy technologies, such as fusion. (MHR)

  17. Observatory Bibliographies as Research Tools

    Science.gov (United States)

    Rots, Arnold H.; Winkelman, S. L.

    2013-01-01

    Traditionally, observatory bibliographies were maintained to provide insight in how successful a observatory is as measured by its prominence in the (refereed) literature. When we set up the bibliographic database for the Chandra X-ray Observatory (http://cxc.harvard.edu/cgi-gen/cda/bibliography) as part of the Chandra Data Archive ((http://cxc.harvard.edu/cda/), very early in the mission, our objective was to make it primarily a useful tool for our user community. To achieve this we are: (1) casting a very wide net in collecting Chandra-related publications; (2) including for each literature reference in the database a wealth of metadata that is useful for the users; and (3) providing specific links between the articles and the datasets in the archive that they use. As a result our users are able to browse the literature and the data archive simultaneously. As an added bonus, the rich metadata content and data links have also allowed us to assemble more meaningful statistics about the scientific efficacy of the observatory. In all this we collaborate closely with the Astrophysics Data System (ADS). Among the plans for future enhancement are the inclusion of press releases and the Chandra image gallery, linking with ADS semantic searching tools, full-text metadata mining, and linking with other observatories' bibliographies. This work is supported by NASA contract NAS8-03060 (CXC) and depends critically on the services provided by the ADS.

  18. Regression: A Bibliography.

    Science.gov (United States)

    Pedrini, D. T.; Pedrini, Bonnie C.

    Regression, another mechanism studied by Sigmund Freud, has had much research, e.g., hypnotic regression, frustration regression, schizophrenic regression, and infra-human-animal regression (often directly related to fixation). Many investigators worked with hypnotic age regression, which has a long history, going back to Russian reflexologists.…

  19. Regression: A Bibliography.

    Science.gov (United States)

    Pedrini, D. T.; Pedrini, Bonnie C.

    Regression, another mechanism studied by Sigmund Freud, has had much research, e.g., hypnotic regression, frustration regression, schizophrenic regression, and infra-human-animal regression (often directly related to fixation). Many investigators worked with hypnotic age regression, which has a long history, going back to Russian reflexologists.…

  20. The case for applying tissue engineering methodologies to instruct human organoid morphogenesis.

    Science.gov (United States)

    Marti-Figueroa, Carlos R; Ashton, Randolph S

    2017-05-01

    Three-dimensional organoids derived from human pluripotent stem cell (hPSC) derivatives have become widely used in vitro models for studying development and disease. Their ability to recapitulate facets of normal human development during in vitro morphogenesis produces tissue structures with unprecedented biomimicry. Current organoid derivation protocols primarily rely on spontaneous morphogenesis processes to occur within 3-D spherical cell aggregates with minimal to no exogenous control. This yields organoids containing microscale regions of biomimetic tissues, but at the macroscale (i.e. 100's of microns to millimeters), the organoids' morphology, cytoarchitecture, and cellular composition are non-biomimetic and variable. The current lack of control over in vitro organoid morphogenesis at the microscale induces aberrations at the macroscale, which impedes realization of the technology's potential to reproducibly form anatomically correct human tissue units that could serve as optimal human in vitro models and even transplants. Here, we review tissue engineering methodologies that could be used to develop powerful approaches for instructing multiscale, 3-D human organoid morphogenesis. Such technological mergers are critically needed to harness organoid morphogenesis as a tool for engineering functional human tissues with biomimetic anatomy and physiology. Human PSC-derived 3-D organoids are revolutionizing the biomedical sciences. They enable the study of development and disease within patient-specific genetic backgrounds and unprecedented biomimetic tissue microenvironments. However, their uncontrolled, spontaneous morphogenesis at the microscale yields inconsistences in macroscale organoid morphology, cytoarchitecture, and cellular composition that limits their standardization and application. Integration of tissue engineering methods with organoid derivation protocols could allow us to harness their potential by instructing standardized in vitro morphogenesis

  1. Human Factors Engineering and Ergonomics Analysis for the Canister Storage Building (CSB) Results and Findings

    Energy Technology Data Exchange (ETDEWEB)

    GARVIN, L.J.

    1999-09-20

    The purpose for this supplemental report is to follow-up and update the information in SNF-3907, Human Factors Engineering (HFE) Analysis: Results and Findings. This supplemental report responds to applicable U.S. Department of Energy Safety Analysis Report review team comments and questions. This Human Factors Engineering and Ergonomics (HFE/Erg) analysis was conducted from April 1999 to July 1999; SNF-3907 was based on analyses accomplished in October 1998. The HFE/Erg findings presented in this report and SNF-3907, along with the results of HNF-3553, Spent Nuclear Fuel Project, Final Safety Analysis Report, Annex A, ''Canister Storage Building Final Safety Analysis Report,'' Chapter A3.0, ''Hazards and Accidents Analyses,'' provide the technical basis for preparing or updating HNF-3553. Annex A, Chaptex A13.0, ''Human Factors Engineering.'' The findings presented in this report allow the HNF-3553 Chapter 13.0, ''Human Factors,'' to respond fully to the HFE requirements established in DOE Order 5480.23, Nuclear Safety Analysis Reports.

  2. Human and mouse tissue-engineered small intestine both demonstrate digestive and absorptive function.

    Science.gov (United States)

    Grant, Christa N; Mojica, Salvador Garcia; Sala, Frederic G; Hill, J Ryan; Levin, Daniel E; Speer, Allison L; Barthel, Erik R; Shimada, Hiroyuki; Zachos, Nicholas C; Grikscheit, Tracy C

    2015-04-15

    Short bowel syndrome (SBS) is a devastating condition in which insufficient small intestinal surface area results in malnutrition and dependence on intravenous parenteral nutrition. There is an increasing incidence of SBS, particularly in premature babies and newborns with congenital intestinal anomalies. Tissue-engineered small intestine (TESI) offers a therapeutic alternative to the current standard treatment, intestinal transplantation, and has the potential to solve its biggest challenges, namely donor shortage and life-long immunosuppression. We have previously demonstrated that TESI can be generated from mouse and human small intestine and histologically replicates key components of native intestine. We hypothesized that TESI also recapitulates native small intestine function. Organoid units were generated from mouse or human donor intestine and implanted into genetically identical or immunodeficient host mice. After 4 wk, TESI was harvested and either fixed and paraffin embedded or immediately subjected to assays to illustrate function. We demonstrated that both mouse and human tissue-engineered small intestine grew into an appropriately polarized sphere of intact epithelium facing a lumen, contiguous with supporting mesenchyme, muscle, and stem/progenitor cells. The epithelium demonstrated major ultrastructural components, including tight junctions and microvilli, transporters, and functional brush-border and digestive enzymes. This study demonstrates that tissue-engineered small intestine possesses a well-differentiated epithelium with intact ion transporters/channels, functional brush-border enzymes, and similar ultrastructural components to native tissue, including progenitor cells, whether derived from mouse or human cells.

  3. Failure Modes and Effects Analysis (FMEA): A Bibliography

    Science.gov (United States)

    2000-01-01

    Failure modes and effects analysis (FMEA) is a bottom-up analytical process that identifies process hazards, which helps managers understand vulnerabilities of systems, as well as assess and mitigate risk. It is one of several engineering tools and techniques available to program and project managers aimed at increasing the likelihood of safe and successful NASA programs and missions. This bibliography references 465 documents in the NASA STI Database that contain the major concepts, failure modes or failure analysis, in either the basic index of the major subject terms.

  4. Human Kunitz-type protease inhibitor engineered for enhanced matrix retention extends longevity of fibrin biomaterials.

    Science.gov (United States)

    Briquez, Priscilla S; Lorentz, Kristen M; Larsson, Hans M; Frey, Peter; Hubbell, Jeffrey A

    2017-08-01

    Aprotinin is a broad-spectrum serine protease inhibitor used in the clinic as an anti-fibrinolytic agent in fibrin-based tissue sealants. However, upon re-exposure, some patients suffer from hypersensitivity immune reactions likely related to the bovine origin of aprotinin. Here, we aimed to develop a human-derived substitute to aprotinin. Based on sequence homology analyses, we identified the Kunitz-type protease inhibitor (KPI) domain of human amyloid-β A4 precursor protein as being a potential candidate. While KPI has a lower intrinsic anti-fibrinolytic activity than aprotinin, we reasoned that its efficacy is additionally limited by its fast release from fibrin material, just as aprotinin's is. Thus, we engineered KPI variants for controlled retention in fibrin biomaterials, using either covalent binding through incorporation of a substrate for the coagulation transglutaminase Factor XIIIa or through engineering of extracellular matrix protein super-affinity domains for sequestration into fibrin. We showed that both engineered KPI variants significantly slowed plasmin-mediated fibrinolysis in vitro, outperforming aprotinin. In vivo, our best engineered KPI variant (incorporating the transglutaminase substrate) extended fibrin matrix longevity by 50%, at a dose at which aprotinin did not show efficacy, thus qualifying it as a competitive substitute of aprotinin in fibrin sealants. Copyright © 2017 The Author(s). Published by Elsevier Ltd.. All rights reserved.

  5. Three-Dimensionally Engineered Normal Human Lung Tissue-Like Assemblies: Target Tissues for Human Respiratory Viral Infections

    Science.gov (United States)

    Goodwin, Thomas J.; McCarthy, M.; Lin, Y-H.; Deatly, A. M.

    2008-01-01

    In vitro three-dimensional (3D) human lung epithelio-mesenchymal tissue-like assemblies (3D hLEM TLAs) from this point forward referred to as TLAs were engineered in Rotating Wall Vessel (RWV) technology to mimic the characteristics of in vivo tissues thus providing a tool to study human respiratory viruses and host cell interactions. The TLAs were bioengineered onto collagen-coated cyclodextran microcarriers using primary human mesenchymal bronchial-tracheal cells (HBTC) as the foundation matrix and an adult human bronchial epithelial immortalized cell line (BEAS-2B) as the overlying component. The resulting TLAs share significant characteristics with in vivo human respiratory epithelium including polarization, tight junctions, desmosomes, and microvilli. The presence of tissue-like differentiation markers including villin, keratins, and specific lung epithelium markers, as well as the production of tissue mucin, further confirm these TLAs differentiated into tissues functionally similar to in vivo tissues. Increasing virus titers for human respiratory syncytial virus (wtRSVA2) and the detection of membrane bound glycoproteins over time confirm productive infection with the virus. Therefore, we assert TLAs mimic aspects of the human respiratory epithelium and provide a unique capability to study the interactions of respiratory viruses and their primary target tissue independent of the host s immune system.

  6. Three-Dimensionally Engineered Normal Human Broncho-epithelial Tissue-Like Assemblies: Target Tissues for Human Respiratory Viral Infections

    Science.gov (United States)

    Goodwin, T. J.; McCarthy, M.; Lin, Y-H

    2006-01-01

    In vitro three-dimensional (3D) human broncho-epithelial (HBE) tissue-like assemblies (3D HBE TLAs) from this point forward referred to as TLAs were engineered in Rotating Wall Vessel (RWV) technology to mimic the characteristics of in vivo tissues thus providing a tool to study human respiratory viruses and host cell interactions. The TLAs were bioengineered onto collagen-coated cyclodextran microcarriers using primary human mesenchymal bronchial-tracheal cells (HBTC) as the foundation matrix and an adult human bronchial epithelial immortalized cell line (BEAS-2B) as the overlying component. The resulting TLAs share significant characteristics with in vivo human respiratory epithelium including polarization, tight junctions, desmosomes, and microvilli. The presence of tissue-like differentiation markers including villin, keratins, and specific lung epithelium markers, as well as the production of tissue mucin, further confirm these TLAs differentiated into tissues functionally similar to in vivo tissues. Increasing virus titers for human respiratory syncytial virus (wtRSVA2) and parainfluenza virus type 3 (wtPIV3 JS) and the detection of membrane bound glycoproteins over time confirm productive infections with both viruses. Therefore, TLAs mimic aspects of the human respiratory epithelium and provide a unique capability to study the interactions of respiratory viruses and their primary target tissue independent of the host's immune system.

  7. Three-Dimensionally Engineered Normal Human Broncho-epithelial Tissue-Like Assemblies: Target Tissues for Human Respiratory Viral Infections

    Science.gov (United States)

    Goodwin, T. J.; McCarthy, M.; Lin, Y-H

    2006-01-01

    In vitro three-dimensional (3D) human broncho-epithelial (HBE) tissue-like assemblies (3D HBE TLAs) from this point forward referred to as TLAs were engineered in Rotating Wall Vessel (RWV) technology to mimic the characteristics of in vivo tissues thus providing a tool to study human respiratory viruses and host cell interactions. The TLAs were bioengineered onto collagen-coated cyclodextran microcarriers using primary human mesenchymal bronchial-tracheal cells (HBTC) as the foundation matrix and an adult human bronchial epithelial immortalized cell line (BEAS-2B) as the overlying component. The resulting TLAs share significant characteristics with in vivo human respiratory epithelium including polarization, tight junctions, desmosomes, and microvilli. The presence of tissue-like differentiation markers including villin, keratins, and specific lung epithelium markers, as well as the production of tissue mucin, further confirm these TLAs differentiated into tissues functionally similar to in vivo tissues. Increasing virus titers for human respiratory syncytial virus (wtRSVA2) and parainfluenza virus type 3 (wtPIV3 JS) and the detection of membrane bound glycoproteins over time confirm productive infections with both viruses. Therefore, TLAs mimic aspects of the human respiratory epithelium and provide a unique capability to study the interactions of respiratory viruses and their primary target tissue independent of the host's immune system.

  8. Engineering and commercialization of human-device interfaces, from bone to brain.

    Science.gov (United States)

    Knothe Tate, Melissa L; Detamore, Michael; Capadona, Jeffrey R; Woolley, Andrew; Knothe, Ulf

    2016-07-01

    Cutting edge developments in engineering of tissues, implants and devices allow for guidance and control of specific physiological structure-function relationships. Yet the engineering of functionally appropriate human-device interfaces represents an intractable challenge in the field. This leading opinion review outlines a set of current approaches as well as hurdles to design of interfaces that modulate transfer of information, i.a. forces, electrical potentials, chemical gradients and haptotactic paths, between endogenous and engineered body parts or tissues. The compendium is designed to bridge across currently separated disciplines by highlighting specific commonalities between seemingly disparate systems, e.g. musculoskeletal and nervous systems. We focus on specific examples from our own laboratories, demonstrating that the seemingly disparate musculoskeletal and nervous systems share common paradigms which can be harnessed to inspire innovative interface design solutions. Functional barrier interfaces that control molecular and biophysical traffic between tissue compartments of joints are addressed in an example of the knee. Furthermore, we describe the engineering of gradients for interfaces between endogenous and engineered tissues as well as between electrodes that physically and electrochemically couple the nervous and musculoskeletal systems. Finally, to promote translation of newly developed technologies into products, protocols, and treatments that benefit the patients who need them most, regulatory and technical challenges and opportunities are addressed on hand from an example of an implant cum delivery device that can be used to heal soft and hard tissues, from brain to bone. Crown Copyright © 2016. Published by Elsevier Ltd. All rights reserved.

  9. Recommendations to the NRC on human engineering guidelines for nuclear power plant maintainability

    Energy Technology Data Exchange (ETDEWEB)

    Badalamente, R.V.; Fecht, B.A.; Blahnik, D.E.; Eklund, J.D.; Hartley, C.S.

    1986-03-01

    This document contains human engineering guidelines which can enhance the maintainability of nuclear power plants. The guidelines have been derived from general human engineering design principles, criteria, and data. The guidelines may be applied to existing plants as well as to plants under construction. They apply to nuclear power plant systems, equipment and facilities, as well as to maintenance tools and equipment. The guidelines are grouped into seven categories: accessibility and workspace, physical environment, loads and forces, maintenance facilities, maintenance tools and equipment, operating equipment design, and information needs. Each chapter of the document details specific maintainability problems encountered at nuclear power plants, the safety impact of these problems, and the specific maintainability design guidelines whose application can serve to avoid these problems in new or existing plants.

  10. 1978 bibliography of atomic and molecular processes. [Bibliography

    Energy Technology Data Exchange (ETDEWEB)

    1980-03-01

    This annotated bibliography lists 2557 works on atomic and molecular processes reported in publications dated 1978. Sources include scientific journals, conference proceedings, and books. Each entry is designated by one or more of the 114 categories of atomic and molecular processes used by the Controlled Fusion Atomic Data Center to classify data. Also indicated is whether the work was experimental or theoretical, what energy range was covered, what reactants were investigated, and the country of origin of the first author. Following the bibliographical listing are indexes of reactants and authors.

  11. 1979 bibliography of atomic and molecular processes. [Bibliography

    Energy Technology Data Exchange (ETDEWEB)

    None

    1980-08-01

    This annotated bibliography lists 2146 works on atomic and molecular processes reported in publications dated 1979. Sources include scientific journals, conference proceedings, and books. Each entry is designated by one or more of the 114 categories of atomic and molecular processes used by the Controlled Fusion Atomic Data Center, Oak Ridge National Laboratory, to classify data. Also indicated is whether the work was experimental or theoretical, what energy range was covered, what reactants were investigated, and the country of origin of the first author. Following the bibliographical listing are indexes of reactants and authors.

  12. Women in Science and Mathematics: Bibliography.

    Science.gov (United States)

    Chinn, Phyllis Zweig, Comp.

    This bibliography contains over 1,000 entries organized into seven sections. These include: (1) "Women in Science Bibliographies"; (2) "Journals and Periodicals Which Have Devoted Whole Issues or Frequent Articles to Women in Sciences"; (3) "Biographies and Autobiographies of Women Scientists"; (4) "Differences Between the Sexes--As Proposed by…

  13. The Anthropology of Dance. A Selected Bibliography.

    Science.gov (United States)

    Hanna, Judith Lynne

    Over 250 monographs, journal articles, and papers are cited in this selected bibliography of resources on the anthropology of dance. Most of the entries were published during the 1960s and 1970s. Entries are arranged alphabetically by author and give information on title, publisher or journal, date, and page numbers. The bibliography is presented…

  14. Comparative and International Education: A Bibliography (2014)

    Science.gov (United States)

    Comparative Education Review, 2015

    2015-01-01

    The 2014 "Comparative Education Review" bibliography of refereed journal articles on topics relevant to comparative and international education covers all 12 months in 2014 and includes 3,389 entries--a full 30 percent increase over the set of references assembled in the 2013 bibliography. They are drawn from 280 refereed journals, both…

  15. Speech Handicapped - Research: Exceptional Child Bibliography Series.

    Science.gov (United States)

    Council for Exceptional Children, Reston, VA. Information Center on Exceptional Children.

    The bibliography, which is one of a series of over 50 similar selected bibliographies dealing with handicapped and gifted children, contains 100 references selected from Exceptional Child Education Abstracts pertaining to research with speech handicapped children. Bibliographic data, availability information, indexing and retrieval terms, and…

  16. Aurally Handicapped - Research: Exceptional Child Bibliography Series.

    Science.gov (United States)

    Council for Exceptional Children, Reston, VA. Information Center on Exceptional Children.

    Eighty-nine references, including research reports, texts, journal articles, and other research-related literature, are included in the bibliography on research concerning aurally handicapped children (both the deaf and the hard of hearing). The bibliography, containing bibliographic data, availability information, indexing and retrieval terms,…

  17. The Basic Course: A Selected, Annotated Bibliography.

    Science.gov (United States)

    Demo, Penny

    Defining basic speech communication courses as those public speaking, interpersonal, or communication courses that treat fundamental communication concepts, this annotated bibliography reflects the current thought of speech educators on the basic course. The bibliography consists of 27 citations, all of which are drawn from the ERIC database. (SKC)

  18. Annotated Bibliography of English for Special Purposes.

    Science.gov (United States)

    Allix, Beverley, Comp.

    This annotated bibliography covers the following types of materials of use to teachers of English for Special Purposes: (1) books, monographs, reports, and conference papers; (2) periodical articles and essays in collections; (3) theses and dissertations; (4) bibliographies; (5) dictionaries; and (6) textbooks in series by publisher. Section (1)…

  19. Bibliography of Utah radioactive occurrences. Volume II

    Energy Technology Data Exchange (ETDEWEB)

    Doelling, H.H. (comp.)

    1983-07-01

    The references in this bibliography were assembled by reviewing published bibliographies of Utah geology, unpublished reports of the US Geological Survey and the Department of Energy, and various university theses. Each of the listings is cross-referenced by location and subject matter. This report is published in two volumes.

  20. Educational Finance (Primary-Secondary Levels) Bibliography.

    Science.gov (United States)

    Dulac, Claude

    This lengthy bibliography in English, French, and German lists documents and journal articles concerning educational finance. Most items were published in Canada and the United States, but listings are also included from France, Germany, Switzerland, and Ceylon. A few items include an ERIC accession number. The bibliography is not annotated and is…

  1. Unidentified Flying Objects, A Selected Bibliography.

    Science.gov (United States)

    Rodgers, Kay, Comp.

    This bibliography, intended for the general reader, provides selective coverage of the unidentified flying object (UFO) literature that has appeared since 1969. The coverage is limited to English language works, but does include translations and materials published abroad. Other bibliographies are listed, as are books, congressional and other…

  2. Ludwig von Mises: An Annotated Bibliography.

    Science.gov (United States)

    Gordon, David

    A 117-item annotated bibliography of books, articles, essays, lectures, and reviews by economist Ludwig von Mises is presented. The bibliography is arranged chronologicaly, and is followed by an alphabetical listing of the citations, excluding books. An index and information on the Ludwig von Mises Institute at Auburn University (Alabama) are…

  3. A Bibliography of Pidgin and Creole Languages.

    Science.gov (United States)

    Reinecke, John E., Comp.; And Others

    This annotated bibliography of pidgin and creole languages is divided into seventeen major sections. They are: bibliographies; collective works; general and miscellaneous works; works concerning pidgins and creoles that are Italian-based, Portuguese-based, Brazilian Portuguese-based, Spanish-based, French-based, Dutch-based, English-based,…

  4. Assessment in Social Work Education: A Bibliography

    Directory of Open Access Journals (Sweden)

    Robert Vernon

    2004-05-01

    Full Text Available We have attempted to provide the reader with a basic bibliography that examines multiple dimensions of assessment. While no bibliography is ever comprehensive, these 270+ references may prove useful to the reader who wishes to further explore this literature.

  5. Bibliography of Utah radioactive occurrences. Volume I

    Energy Technology Data Exchange (ETDEWEB)

    Doelling, H.H. (comp.)

    1983-07-01

    The references in this bibliography were assembled by reviewing published bibliographies of Utah geology, unpublished reports of the US Geological Survey and the Department of Energy, and various university theses. Each of the listings is cross-referenced by location and subject matter. This report is published in two volumes.

  6. Annotated bibliography of LAMPF research and development

    Energy Technology Data Exchange (ETDEWEB)

    Jameson, R.A.; Roybal, E.U.

    1978-08-01

    A bibliography on published and in-house technical material written on LAMPF activities since its inception is presented. Subject and author concordances provide cross-reference to detailed citations, which include an abstract and notes on the material. The bibliography resides in a computer database that can be searched for key words and phrases.

  7. FlyBase: improvements to the bibliography.

    Science.gov (United States)

    Marygold, Steven J; Leyland, Paul C; Seal, Ruth L; Goodman, Joshua L; Thurmond, Jim; Strelets, Victor B; Wilson, Robert J

    2013-01-01

    An accurate, comprehensive, non-redundant and up-to-date bibliography is a crucial component of any Model Organism Database (MOD). Principally, the bibliography provides a set of references that are specific to the field served by the MOD. Moreover, it serves as a backbone to which all curated biological data can be attributed. Here, we describe the organization and main features of the bibliography in FlyBase (flybase.org), the MOD for Drosophila melanogaster. We present an overview of the current content of the bibliography, the pipeline for identifying and adding new references, the presentation of data within Reference Reports and effective methods for searching and retrieving bibliographic data. We highlight recent improvements in these areas and describe the advantages of using the FlyBase bibliography over alternative literature resources. Although this article is focused on bibliographic data, many of the features and tools described are applicable to browsing and querying other datasets in FlyBase.

  8. Workshop on cooperative and human aspects of software engineering (CHASE 2011)

    DEFF Research Database (Denmark)

    Cataldo, Marcelo; de Souza, Cleidson; Dittrich, Yvonne

    2011-01-01

    is to provide a forum for discussing high quality research on human and cooperative aspects of software engineering. We aim at providing both a meeting place for the growing community and the possibility for researchers interested in joining the field to present their work in progress and get an overview over......Software is created by people for people working in varied environments, under various conditions. Thus understanding cooperative and human aspects of software development is crucial to comprehend how methods and tools are used, and thereby improve the creation and maintenance of software. Over...

  9. Workshop on cooperative and human aspects of software engineering (CHASE 2011)

    DEFF Research Database (Denmark)

    Cataldo, Marcelo; de Souza, Cleidson; Dittrich, Yvonne

    2011-01-01

    is to provide a forum for discussing high quality research on human and cooperative aspects of software engineering. We aim at providing both a meeting place for the growing community and the possibility for researchers interested in joining the field to present their work in progress and get an overview over......Software is created by people for people working in varied environments, under various conditions. Thus understanding cooperative and human aspects of software development is crucial to comprehend how methods and tools are used, and thereby improve the creation and maintenance of software. Over...

  10. Scaffold-free cartilage tissue engineering with a small population of human nasoseptal chondrocytes.

    Science.gov (United States)

    Chiu, Loraine L Y; To, William T H; Lee, John M; Waldman, Stephen D

    2017-03-01

    Cartilage tissue engineering is a promising approach to provide suitable materials for nasal reconstruction; however, it typically requires large numbers of cells. We have previously shown that a small number of chondrocytes cultivated within a continuous flow bioreactor can elicit substantial tissue growth, but translation to human chondrocytes is not trivial. Here, we aimed to demonstrate the application of the bioreactor to generate large-sized tissues from a small population of primary human nasoseptal chondrocytes. Experimental study. Chondrocytes were cultured in the bioreactor using different medium compositions, with varying amounts of serum and with or without growth factors. Resulting engineered tissues were analyzed for physical properties, biochemical composition, tissue microstructure, and protein localization. Bioreactor-cultivated constructs grown with serum and growth factors (basic fibroblast growth factor and transforming growth factor beta 2) had greater thickness, as well as DNA and glycosaminoglycan (GAG) contents, compared to low serum and no growth factor controls. These constructs also showed the most intense proteoglycan and collagen II staining. The combination of bioreactor conditions, serum, and growth factors allowed the generation of large, thick scaffold-free human cartilaginous tissues that resembled the native nasoseptal cartilage. There also may be implications for patient selection in future clinical applications of these engineered tissues because their GAG content decreased with donor age. NA. Laryngoscope, 127:E91-E99, 2017. © 2016 The American Laryngological, Rhinological and Otological Society, Inc.

  11. Engineering and Humanities Students' Strategies for Vocabulary Acquisition: An Iranian Experience

    Directory of Open Access Journals (Sweden)

    Hassan Soodmand Afshar

    2014-05-01

    Full Text Available The present study set out to investigate the differences between EAP (English for Academic Purposes students of Humanities and Engineering in terms of vocabulary strategy choice and use. One hundred and five undergraduate Iranian students (39 students from Engineering Faculty and 66 from Humanities Faculty studying at Bu-Ali Sina University Hamedan, during the academic year of 2011–2012 participated in this study. For data collection purposes, a pilot-tested factor-analyzed five-point Likert-scale vocabulary learning strategies questionnaire (VLSQ containing 45 statements was adopted. The results of independent samples t-test indicated that, overall, the two groups were not significantly different in the choice and use of vocabulary learning strategies. However, running Chi square analyses, significant differences were found in individual strategy use in 6 out of 45 strategies. That is, while Humanities students used more superficial and straightforward strategies like repetition strategy and seeking help from others, the Engineering students preferred much deeper, thought-provoking and sophisticated strategies like using a monolingual dictionary and learning vocabulary through collocations and coordinates. Further, the most and the least frequently used vocabulary learning strategies by the two groups were specified, out of which only two strategies in each category were commonly shared by both groups. The possible reasons why the results have turned out to be so as well as the implications of the study are discussed in details in the paper.

  12. Noninvasive metabolic imaging of engineered 3D human adipose tissue in a perfusion bioreactor.

    Directory of Open Access Journals (Sweden)

    Andrew Ward

    Full Text Available The efficacy and economy of most in vitro human models used in research is limited by the lack of a physiologically-relevant three-dimensional perfused environment and the inability to noninvasively quantify the structural and biochemical characteristics of the tissue. The goal of this project was to develop a perfusion bioreactor system compatible with two-photon imaging to noninvasively assess tissue engineered human adipose tissue structure and function in vitro. Three-dimensional (3D vascularized human adipose tissues were engineered in vitro, before being introduced to a perfusion environment and tracked over time by automated quantification of endogenous markers of metabolism using two-photon excited fluorescence (TPEF. Depth-resolved image stacks were analyzed for redox ratio metabolic profiling and compared to prior analyses performed on 3D engineered adipose tissue in static culture. Traditional assessments with H&E staining were used to qualitatively measure extracellular matrix generation and cell density with respect to location within the tissue. The distribution of cells within the tissue and average cellular redox ratios were different between static and perfusion cultures, while the trends of decreased redox ratio and increased cellular proliferation with time in both static and perfusion cultures were similar. These results establish a basis for noninvasive optical tracking of tissue structure and function in vitro, which can be applied to future studies to assess tissue development or drug toxicity screening and disease progression.

  13. Noninvasive metabolic imaging of engineered 3D human adipose tissue in a perfusion bioreactor.

    Science.gov (United States)

    Ward, Andrew; Quinn, Kyle P; Bellas, Evangelia; Georgakoudi, Irene; Kaplan, David L

    2013-01-01

    The efficacy and economy of most in vitro human models used in research is limited by the lack of a physiologically-relevant three-dimensional perfused environment and the inability to noninvasively quantify the structural and biochemical characteristics of the tissue. The goal of this project was to develop a perfusion bioreactor system compatible with two-photon imaging to noninvasively assess tissue engineered human adipose tissue structure and function in vitro. Three-dimensional (3D) vascularized human adipose tissues were engineered in vitro, before being introduced to a perfusion environment and tracked over time by automated quantification of endogenous markers of metabolism using two-photon excited fluorescence (TPEF). Depth-resolved image stacks were analyzed for redox ratio metabolic profiling and compared to prior analyses performed on 3D engineered adipose tissue in static culture. Traditional assessments with H&E staining were used to qualitatively measure extracellular matrix generation and cell density with respect to location within the tissue. The distribution of cells within the tissue and average cellular redox ratios were different between static and perfusion cultures, while the trends of decreased redox ratio and increased cellular proliferation with time in both static and perfusion cultures were similar. These results establish a basis for noninvasive optical tracking of tissue structure and function in vitro, which can be applied to future studies to assess tissue development or drug toxicity screening and disease progression.

  14. Efficient CRISPR/Cas9-based Genome Engineering in Human Pluripotent Stem Cells

    Science.gov (United States)

    Kime, Cody; Mandegar, Mohammad A.; Srivastava, Deepak; Yamanaka, Shinya; Conklin, Bruce R.; Rand, Tim A.

    2016-01-01

    Human pluripotent stem cells (hPSCs) are rapidly emerging as a powerful tool for biomedical discovery. The advent of human induced pluripotent stem (hiPS) cells with human embryonic stem (hES) cell-like properties has led to hPSCs with disease-specific genetic backgrounds for in-vitro disease modeling, drug discovery, mechanistic and developmental studies. To fully realize this potential it will be necessary to modify the genome of hPSCs with precision and flexibility. Pioneering experiments utilizing site-specific double strand break (DSB)-mediated genome engineering tools, including Zinc Finger Nucleases (ZFNs) and Transcription Activator-Like Effector Nucleases (TALENs), have paved the way to genome engineering in previously recalcitrant systems such as hPSCs. However, these methods are technically cumbersome and require significant expertise, which limited adoption. A major recent advance involving the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) endonuclease has dramatically simplified the effort required for genome engineering and will likely be adopted widely as the most rapid and flexible system for genome editing in hPSCs. Herein, we describe commonly practiced methods for CRISPR endonuclease genomic editing of hPSCs to cell lines containing genomes altered by Insertion/Deletion (INDEL) mutagenesis or insertion of recombinant genomic DNA. PMID:26724721

  15. Efficient CRISPR/Cas9-Based Genome Engineering in Human Pluripotent Stem Cells.

    Science.gov (United States)

    Kime, Cody; Mandegar, Mohammad A; Srivastava, Deepak; Yamanaka, Shinya; Conklin, Bruce R; Rand, Tim A

    2016-01-01

    Human pluripotent stem cells (hPS cells) are rapidly emerging as a powerful tool for biomedical discovery. The advent of human induced pluripotent stem cells (hiPS cells) with human embryonic stem (hES)-cell-like properties has led to hPS cells with disease-specific genetic backgrounds for in vitro disease modeling and drug discovery as well as mechanistic and developmental studies. To fully realize this potential, it will be necessary to modify the genome of hPS cells with precision and flexibility. Pioneering experiments utilizing site-specific double-strand break (DSB)-mediated genome engineering tools, including zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs), have paved the way to genome engineering in previously recalcitrant systems such as hPS cells. However, these methods are technically cumbersome and require significant expertise, which has limited adoption. A major recent advance involving the clustered regularly interspaced short palindromic repeats (CRISPR) endonuclease has dramatically simplified the effort required for genome engineering and will likely be adopted widely as the most rapid and flexible system for genome editing in hPS cells. In this unit, we describe commonly practiced methods for CRISPR endonuclease genomic editing of hPS cells into cell lines containing genomes altered by insertion/deletion (indel) mutagenesis or insertion of recombinant genomic DNA.

  16. The Design of the Internet's Architecture by the Internet Engineering Task Force (IETF) and Human Rights.

    Science.gov (United States)

    Cath, Corinne; Floridi, Luciano

    2017-04-01

    The debate on whether and how the Internet can protect and foster human rights has become a defining issue of our time. This debate often focuses on Internet governance from a regulatory perspective, underestimating the influence and power of the governance of the Internet's architecture. The technical decisions made by Internet Standard Developing Organisations (SDOs) that build and maintain the technical infrastructure of the Internet influences how information flows. They rearrange the shape of the technically mediated public sphere, including which rights it protects and which practices it enables. In this article, we contribute to the debate on SDOs' ethical responsibility to bring their work in line with human rights. We defend three theses. First, SDOs' work is inherently political. Second, the Internet Engineering Task Force (IETF), one of the most influential SDOs, has a moral obligation to ensure its work is coherent with, and fosters, human rights. Third, the IETF should enable the actualisation of human rights through the protocols and standards it designs by implementing a responsibility-by-design approach to engineering. We conclude by presenting some initial recommendations on how to ensure that work carried out by the IETF may enable human rights.

  17. Meganucleases Revolutionize the Production of Genetically Engineered Pigs for the Study of Human Diseases.

    Science.gov (United States)

    Redel, Bethany K; Prather, Randall S

    2016-04-01

    Animal models of human diseases are critically necessary for developing an in-depth knowledge of disease development and progression. In addition, animal models are vital to the development of potential treatments or even cures for human diseases. Pigs are exceptional models as their size, physiology, and genetics are closer to that of humans than rodents. In this review, we discuss the use of pigs in human translational research and the evolving technology that has increased the efficiency of genetically engineering pigs. With the emergence of the clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPR-associated (Cas) protein 9 system technology, the cost and time it takes to genetically engineer pigs has markedly decreased. We will also discuss the use of another meganuclease, the transcription activator-like effector nucleases , to produce pigs with severe combined immunodeficiency by developing targeted modifications of the recombination activating gene 2 (RAG2).RAG2mutant pigs may become excellent animals to facilitate the development of xenotransplantation, regenerative medicine, and tumor biology. The use of pig biomedical models is vital for furthering the knowledge of, and for treating human, diseases.

  18. Engineering antigen-specific T cells from genetically modified human hematopoietic stem cells in immunodeficient mice.

    Directory of Open Access Journals (Sweden)

    Scott G Kitchen

    Full Text Available There is a desperate need for effective therapies to fight chronic viral infections. The immune response is normally fastidious at controlling the majority of viral infections and a therapeutic strategy aimed at reestablishing immune control represents a potentially powerful approach towards treating persistent viral infections. We examined the potential of genetically programming human hematopoietic stem cells to generate mature CD8+ cytotoxic T lymphocytes that express a molecularly cloned, "transgenic" human anti-HIV T cell receptor (TCR. Anti-HIV TCR transduction of human hematopoietic stem cells directed the maturation of a large population of polyfunctional, HIV-specific CD8+ cells capable of recognizing and killing viral antigen-presenting cells. Thus, through this proof-of-concept we propose that genetic engineering of human hematopoietic stem cells will allow the tailoring of effector T cell responses to fight HIV infection or other diseases that are characterized by the loss of immune control.

  19. Applications of human factors engineering to LNG release prevention and control

    Energy Technology Data Exchange (ETDEWEB)

    Shikiar, R.; Rankin, W.L.; Rideout, T.B.

    1982-06-01

    The results of an investigation of human factors engineering and human reliability applications to LNG release prevention and control are reported. The report includes a discussion of possible human error contributions to previous LNG accidents and incidents, and a discussion of generic HF considerations for peakshaving plants. More specific recommendations for improving HF practices at peakshaving plants are offered based on visits to six facilities. The HF aspects of the recently promulgated DOT regulations are reviewed, and recommendations are made concerning how these regulations can be implemented utilizing standard HF practices. Finally, the integration of HF considerations into overall system safety is illustrated by a presentation of human error probabilities applicable to LNG operations and by an expanded fault tree analysis which explicitly recognizes man-machine interfaces.

  20. [Studies on genetic engineering of human insulin-purification and characterization of human proinsulin and insulin].

    Science.gov (United States)

    Guo, L H; Zhou, M Y; Shen, M H; Wang, E B; Liu, J F; Yu, Y J

    1992-06-01

    E. coli DH 5 alpha cells harboring a plasmid pWR 590-BCA 4 for fused human proinsulin production were cultured. The fused human proinsulin was isolated from the fermented cells and then subjected it to cleavage with BrCN. The cleaved product was then converted to crude proinsulin-S-sulfonate using oxidative sulfitolysis. The isolation of human proinsulin-S-sulfonate was accomplished by ion exchange chromatography on QAE-sephadex A-25, followed by gel filtration on sephadex G-50. The purified human proinsulin-S-sulfonate was folded using a disulfide interchange method. The folding mixture was then chromatographed on sephadex G-50 and purified proinsulin was obtained. The proinsulin was then converted to human insulin and C-peptide by a combination cleavage with trypsin and carboxypeptidase B. The total yield of human insulin was about 5 mg/L The Zinc insulin crystals were obtained with amorphous human insulin using citrate method. The amino acid composition N-terminal sequences as well as C-terminal amino acid residues are in agreement with expected results. The hypoglycemic activity of purified human insulin is 26-27 U/mg, as judged by mouse convulsion assay, and the RIA activity is about 99% of that of porcine insulin.

  1. Annotated Bibliography of Research in the Teaching of English

    Science.gov (United States)

    Beach, Richard; Brendler, Beth; Dillon, Deborah; Dockter, Jessie; Ernst, Stacy; Frederick, Amy; Galda, Lee; Helman, Lori; Kapoor, Richa; Ngo, Bic; O'Brien, David; Scharber, Cassie; Jorgensen, Karen; Liang, Lauren; Braaksma, Martine; Janssen, Tanja

    2010-01-01

    This article presents an annotated bibliography of "Research in the Teaching of English" (RTE). The 2010 version of the bibliography involves a major change--the bibliography is available solely as a downloadable pdf file at http://www.ncte.org/journals/rte/issues/v45-2. As the length of the bibliography has grown from 15 pages in 2003 to 88 pages…

  2. Species-Specific Chromosome Engineering Greatly Improves Fully Human Polyclonal Antibody Production Profile in Cattle.

    Directory of Open Access Journals (Sweden)

    Hiroaki Matsushita

    Full Text Available Large-scale production of fully human IgG (hIgG or human polyclonal antibodies (hpAbs by transgenic animals could be useful for human therapy. However, production level of hpAbs in transgenic animals is generally very low, probably due to the fact that evolutionarily unique interspecies-incompatible genomic sequences between human and non-human host species may impede high production of fully hIgG in the non-human environment. To address this issue, we performed species-specific human artificial chromosome (HAC engineering and tested these engineered HAC in cattle. Our previous study has demonstrated that site-specific genomic chimerization of pre-B cell receptor/B cell receptor (pre-BCR/BCR components on HAC vectors significantly improves human IgG expression in cattle where the endogenous bovine immunoglobulin genes were knocked out. In this report, hIgG1 class switch regulatory elements were subjected to site-specific genomic chimerization on HAC vectors to further enhance hIgG expression and improve hIgG subclass distribution in cattle. These species-specific modifications in a chromosome scale resulted in much higher production levels of fully hIgG of up to 15 g/L in sera or plasma, the highest ever reported for a transgenic animal system. Transchromosomic (Tc cattle containing engineered HAC vectors generated hpAbs with high titers against human-origin antigens following immunization. This study clearly demonstrates that species-specific sequence differences in pre-BCR/BCR components and IgG1 class switch regulatory elements between human and bovine are indeed functionally distinct across the two species, and therefore, are responsible for low production of fully hIgG in our early versions of Tc cattle. The high production levels of fully hIgG with hIgG1 subclass dominancy in a large farm animal species achieved here is an important milestone towards broad therapeutic applications of hpAbs.

  3. A focused bibliography on robotics

    Science.gov (United States)

    Mergler, H. W.

    1983-08-01

    The present bibliography focuses on eight robotics-related topics believed by the author to be of special interest to researchers in the field of industrial electronics: robots, sensors, kinematics, dynamics, control systems, actuators, vision, economics, and robot applications. This literature search was conducted through the 1970-present COMPENDEX data base, which provides world-wide coverage of nearly 3500 journals, conference proceedings and reports, and the 1969-1981 INSPEC data base, which is the largest for the English language in the fields of physics, electrotechnology, computers, and control.

  4. Cognitive work analysis: An influential legacy extending beyond human factors and engineering.

    Science.gov (United States)

    Naikar, Neelam

    2017-03-01

    Jens Rasmussen's multifaceted legacy includes cognitive work analysis (CWA), a framework for the analysis, design, and evaluation of complex sociotechnical systems. After considering the framework's origins, this paper reviews its progress, predictably covering experimental research on ecological interface design, case studies of the application of CWA to human factors and engineering problems in industry, and methods and modelling tools for CWA. Emphasis is placed, however, on studying the nexus between some of the recent results obtained with CWA and the original field studies of human problem-solving that motivated the framework's development. Of particular interest is a case study of the use of CWA for military doctrine development, a problem commonly regarded as lying outside the fields of human factors and engineering. It is concluded that the value of CWA, even for such diverse problems, is likely to result from its conceptual grounding in empirical observations of patterns of human reasoning in complex systems. Crown Copyright © 2016. Published by Elsevier Ltd. All rights reserved.

  5. Regenerative potential of human airway stem cells in lung epithelial engineering.

    Science.gov (United States)

    Gilpin, Sarah E; Charest, Jonathan M; Ren, Xi; Tapias, Luis F; Wu, Tong; Evangelista-Leite, Daniele; Mathisen, Douglas J; Ott, Harald C

    2016-11-01

    Bio-engineered organs for transplantation may ultimately provide a personalized solution for end-stage organ failure, without the risk of rejection. Building upon the process of whole organ perfusion decellularization, we aimed to develop novel, translational methods for the recellularization and regeneration of transplantable lung constructs. We first isolated a proliferative KRT5(+)TP63(+) basal epithelial stem cell population from human lung tissue and demonstrated expansion capacity in conventional 2D culture. We then repopulated acellular rat scaffolds in ex vivo whole organ culture and observed continued cell proliferation, in combination with primary pulmonary endothelial cells. To show clinical scalability, and to test the regenerative capacity of the basal cell population in a human context, we then recellularized and cultured isolated human lung scaffolds under biomimetic conditions. Analysis of the regenerated tissue constructs confirmed cell viability and sustained metabolic activity over 7 days of culture. Tissue analysis revealed extensive recellularization with organized tissue architecture and morphology, and preserved basal epithelial cell phenotype. The recellularized lung constructs displayed dynamic compliance and rudimentary gas exchange capacity. Our results underline the regenerative potential of patient-derived human airway stem cells in lung tissue engineering. We anticipate these advances to have clinically relevant implications for whole lung bioengineering and ex vivo organ repair.

  6. Engineering humanized antibody framework sequences for optimal site-specific conjugation of cytotoxins.

    Science.gov (United States)

    Spidel, Jared L; Albone, Earl F; Cheng, Xin; Vaessen, Benjamin; Jacob, Sara; Milinichik, Andrew Z; Verdi, Arielle; Kline, J Bradford; Grasso, Luigi

    The prevailing techniques used to generate antibody-drug conjugates (ADCs) involve random conjugation of the linker-drug to multiple lysines or cysteines in the antibody. Engineering natural and non-natural amino acids into an antibody has been demonstrated to be an effective strategy to produce homogeneous ADC products with defined drug-to-antibody ratios. We recently reported an efficient residue-specific conjugation technology (RESPECT) where thiol-reactive payloads can be efficiently conjugated to a native unpaired cysteine in position 80 (C80) of rabbit light chains. Deimmunizing the rabbit variable domains through humanization is necessary to reduce the risk of anti-drug antibody responses in patients. However, we found that first-generation humanized RESPECT ADCs showed high levels of aggregation and low conjugation efficiency. We correlated these negative properties to the phenylalanine at position 83 present in most human variable kappa frameworks. When position 83 was substituted with selected amino acids, conjugation was restored and aggregation was reduced to levels similar to the chimeric ADC. This engineering strategy allows for development of second-generation humanized RESPECT ADCs with desirable biopharmaceutical properties.

  7. Human Factors Engineering Requirements for the International Space Station - Successes and Challenges

    Science.gov (United States)

    Whitmore, M.; Blume, J.

    2003-01-01

    Advanced technology coupled with the desire to explore space has resulted in increasingly longer human space missions. Indeed, any exploration mission outside of Earth's neighborhood, in other words, beyond the moon, will necessarily be several months or even years. The International Space Station (ISS) serves as an important advancement toward executing a successful human space mission that is longer than a standard trip around the world or to the moon. The ISS, which is a permanently occupied microgravity research facility orbiting the earth, will support missions four to six months in duration. In planning for the ISS, the NASA developed an agency-wide set of human factors standards for the first time in a space exploration program. The Man-Systems Integration Standard (MSIS), NASA-STD-3000, a multi-volume set of guidelines for human-centered design in microgravity, was developed with the cooperation of human factors experts from various NASA centers, industry, academia, and other government agencies. The ISS program formed a human factors team analogous to any major engineering subsystem. This team develops and maintains the human factors requirements regarding end-to-end architecture design and performance, hardware and software design requirements, and test and verification requirements. It is also responsible for providing program integration across all of the larger scale elements, smaller scale hardware, and international partners.

  8. In Genes We Trust: Germline Engineering, Eugenics, and the Future of the Human Genome.

    Science.gov (United States)

    Powell, Russell

    2015-12-01

    Liberal proponents of genetic engineering maintain that developing human germline modification technologies is morally desirable because it will result in a net improvement in human health and well-being. Skeptics of germline modification, in contrast, fear evolutionary harms that could flow from intervening in the human germline, and worry that such programs, even if well intentioned, could lead to a recapitulation of the scientifically and morally discredited projects of the old eugenics. Some bioconservatives have appealed as well to the value of retaining our "given" human biological nature as a reason for restraining the development and use of human genetic modification technologies even where they would tend to increase well-being. In this article, I argue that germline intervention will be necessary merely to sustain the levels of genetic health that we presently enjoy for future generations-a goal that should appeal to bioliberals and bioconservatives alike. This is due to the population-genetic consequences of relaxed selection pressures in human populations caused by the increasing efficacy and availability of conventional medicine. This heterodox conclusion, which I present as a problem of intergenerational justice, has been overlooked in medicine and bioethics due to certain misconceptions about human evolution, which I attempt to rectify, as well as the sordid history of Darwinian approaches to medicine and social policy, which I distinguish from the present argument.

  9. Development and characterization of decellularized human nasoseptal cartilage matrix for use in tissue engineering.

    Science.gov (United States)

    Graham, M Elise; Gratzer, Paul F; Bezuhly, Michael; Hong, Paul

    2016-10-01

    Reconstruction of cartilage defects in the head and neck can require harvesting of autologous cartilage grafts, which can be associated with donor site morbidity. To overcome this limitation, tissue-engineering approaches may be used to generate cartilage grafts. The objective of this study was to decellularize and characterize human nasoseptal cartilage with the aim of generating a biological scaffold for cartilage tissue engineering. Laboratory study using nasoseptal cartilage. Remnant human nasoseptal cartilage specimens were collected and subjected to a novel decellularization treatment. The decellularization process involved several cycles of enzymatic detergent treatments. For characterization, decellularized and fresh (control) specimens underwent histological, biochemical, and mechanical analyses. Scanning electron microscopy and biocompatibility assay were also performed. The decellularization process had minimal effect on glycosaminoglycan content of the cartilage extracellular matrix. Deoxyribonucleic acid (DNA) analysis revealed the near-complete removal of genomic DNA from decellularized tissues. The effectiveness of the decellularization process was also confirmed on histological and scanning electron microscopic analyses. Mechanical testing results showed that the structural integrity of the decellularized tissue was maintained, and biocompatibility was confirmed. Overall, the current decellularization treatment resulted in significant reduction of genetic/cellular material with preservation of the underlying extracellular matrix structure. This decellularized material may serve as a potential scaffold for cartilage tissue engineering. N/A. Laryngoscope, 126:2226-2231, 2016. © 2016 The American Laryngological, Rhinological and Otological Society, Inc.

  10. Human Factors for Clinical Engineering - The Realization of a Guidance Text

    Institute of Scientific and Technical Information of China (English)

    A C Easty; A L Cassano-Pich; M Griffin; Y-L Lin; P Trbovich

    2016-01-01

    Industries such as aviation and nuclear power have greatly improved their safety performance through the application of human factors methods to the design, development, selection and deployment of a range of technologies and work processes. Health care safety performance generally lags behind these industries and would benefit from a similar application of human factors methods. Clinical engineers are in an ideal position to acquire and apply this human factors knowledge, and lead its adoption in health care. This paper describes a text that has been written speciifcally for clinical engineers and others who design, develop, select and support the use of health care technologies, to enable them to learn the key methods of human factors and adopt them as part of their ongoing work. Early indications are that these approaches help to ensure that health care technologies are used more safely and effectively, and it is hoped that large-scale adoption will result in a noticeable and worthwhile improvement in overall health care safety. The described text is now ready and has been published in English on the IFMBE website. It is available as a free download in PDF format. Clinical engineers and others working around the world in the area of health technology are encouraged to learn and adopt these methods, and use them as appropriate in their local setting. At time of writing, plans are underway for a translation of this book into Spanish. Once completed, this version will also be made available online at no charge. The authors encourage readers to contact them with their experiences, and the aim is to build a worldwide community that gradually adopts these methods and helps to drive safety improvements in health care.

  11. Metabolic engineering of Salmonella vaccine bacteria to boost human Vγ2Vδ2 T cell immunity.

    Science.gov (United States)

    Workalemahu, Grefachew; Wang, Hong; Puan, Kia-Joo; Nada, Mohanad H; Kuzuyama, Tomohisa; Jones, Bradley D; Jin, Chenggang; Morita, Craig T

    2014-07-15

    Human Vγ2Vδ2 T cells monitor isoprenoid metabolism by recognizing foreign (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMBPP), a metabolite in the 2-C-methyl-D-erythritol-4-phosphate pathway used by most eubacteria and apicomplexan parasites, and self isopentenyl pyrophosphate, a metabolite in the mevalonate pathway used by humans. Whereas microbial infections elicit prolonged expansion of memory Vγ2Vδ2 T cells, immunization with prenyl pyrophosphates or aminobisphosphonates elicit short-term Vγ2Vδ2 expansion with rapid anergy and deletion upon subsequent immunizations. We hypothesized that a live, attenuated bacterial vaccine that overproduces HMBPP would elicit long-lasting Vγ2Vδ2 T cell immunity by mimicking a natural infection. Therefore, we metabolically engineered the avirulent aroA(-) Salmonella enterica serovar Typhimurium SL7207 strain by deleting the gene for LytB (the downstream enzyme from HMBPP) and functionally complementing for this loss with genes encoding mevalonate pathway enzymes. LytB(-) Salmonella SL7207 had high HMBPP levels, infected human cells as efficiently as did the wild-type bacteria, and stimulated large ex vivo expansions of Vγ2Vδ2 T cells from human donors. Importantly, vaccination of a rhesus monkey with live lytB(-) Salmonella SL7207 stimulated a prolonged expansion of Vγ2Vδ2 T cells without significant side effects or anergy induction. These studies provide proof-of-principle that metabolic engineering can be used to derive live bacterial vaccines that boost Vγ2Vδ2 T cell immunity. Similar engineering of metabolic pathways to produce lipid Ags or B vitamin metabolite Ags could be used to derive live bacterial vaccine for other unconventional T cells that recognize nonpeptide Ags.

  12. 3D engineered cardiac tissue models of human heart disease: learning more from our mice.

    Science.gov (United States)

    Ralphe, J Carter; de Lange, Willem J

    2013-02-01

    Mouse engineered cardiac tissue constructs (mECTs) are a new tool available to study human forms of genetic heart disease within the laboratory. The cultured strips of cardiac cells generate physiologic calcium transients and twitch force, and respond to electrical pacing and adrenergic stimulation. The mECT can be made using cells from existing mouse models of cardiac disease, providing a robust readout of contractile performance and allowing a rapid assessment of genotype-phenotype correlations and responses to therapies. mECT represents an efficient and economical extension to the existing tools for studying cardiac physiology. Human ECTs generated from iPSCMs represent the next logical step for this technology and offer significant promise of an integrated, fully human, cardiac tissue model.

  13. KEPCO‧s Activity to Power-Engineer Human Resource Development

    Science.gov (United States)

    Kobashi, Kazushi

    While business environment changes a lot, in order to aim at realization of “what we want the Group to look like in 2030” , it is necessary to cultivate human resources with a strong sense of mission. We need to prepare an opportunity to teach and to be taught, in order to cultivate resources and a measure for connecting every person‧s growth to growth of a company. In chapter one, we show Five Trends for attaining what KANSAI Electric Power Corporation wants to be and explain the importance of human resource development under the changing environment. In chapter two, we explain the fundamental policy of human resource cultivation and describe the development plan and the facilities for training based on the policy in chapter two. In chapter three, we express the specific efforts in the field of maintenance, construction, and operation at the department of Engineering and Operation.

  14. Genetic engineering of mesenchymal stem cells and its application in human disease therapy.

    Science.gov (United States)

    Hodgkinson, Conrad P; Gomez, José A; Mirotsou, Maria; Dzau, Victor J

    2010-11-01

    The use of stem cells for tissue regeneration and repair is advancing both at the bench and bedside. Stem cells isolated from bone marrow are currently being tested for their therapeutic potential in a variety of clinical conditions including cardiovascular injury, kidney failure, cancer, and neurological and bone disorders. Despite the advantages, stem cell therapy is still limited by low survival, engraftment, and homing to damage area as well as inefficiencies in differentiating into fully functional tissues. Genetic engineering of mesenchymal stem cells is being explored as a means to circumvent some of these problems. This review presents the current understanding of the use of genetically engineered mesenchymal stem cells in human disease therapy with emphasis on genetic modifications aimed to improve survival, homing, angiogenesis, and heart function after myocardial infarction. Advancements in other disease areas are also discussed.

  15. Towards breaking the silence between the two cultures: Engineering and the other humanities

    Energy Technology Data Exchange (ETDEWEB)

    Prausnitz, John M.

    2003-01-01

    Over the years, I have attended numerous meetings like this one at the Center for the Study of Higher Education. I have noticed that most of the attendees, and certainly the speakers, tend to come from the social sciences or humanities. Only rarely do I see anyone here from Berkeley's College of Chemistry or College of Engineering. I come from the College of Chemistry that includes Berkeley's Department of Chemical Engineering. I mention this background to indicate that my remarks here are necessarily less abstract, less theoretical and less philosophical than those of most previous seminar speakers. My remarks are probably somewhat simplistic because, as a result of my engineering background, I tend to focus less on generalities and principles, giving more attention to possible solutions of limited practical problems. About seven weeks ago, I was invited to attend a conference sponsored by the Berlin Academy of Sciences where ''Sciences'' is not confined to natural sciences but includes also humanities and social sciences. The topic of the Conference was ''Sprachlosigkeit'', a German word that roughly translated means inability to speak. The subtitle was ''Silence Between the Disciplines''. The German universities are worried about the increasing gulf between what is often called ''the two cultures''. This gulf is a problem everywhere, including Berkeley, but it is my impression that it is much worse in Europe than in America. The International Conference in Berlin was attended by some big names including the presidents of the Humboldt University in Berlin, the University of Uppsala in Sweden and the Central European University of Budapest, as well as some distinguished academics from a variety of institutions including Harvard and Stanford, and the presidents of three major funding organizations: The Volkswagen Foundation, The German National Science Foundation and the Max

  16. Natural and genetically engineered viral agents for oncolysis and gene therapy of human cancers.

    Science.gov (United States)

    Sinkovics, Joseph G; Horvath, Joseph C

    2008-12-01

    Based on personal acquaintances and experience dating back to the early 1950s, the senior author reviews the history of viral therapy of cancer. He points out the difficulties encountered in the treatment of human cancers, as opposed by the highly successful viral therapy of experimentally maintained tumors in laboratory animals, especially that of ascites carcinomas in mice. A detailed account of viral therapy of human tumors with naturally oncolytic viruses follows, emphasizing the first clinical trials with viral oncolysates. The discrepancy between the high success rates, culminating in cures, in the treatment of tumors of laboratory animals, and the moderate results, such as stabilizations of disease, partial responses, very rare complete remissions, and frequent relapses with virally treated human tumors is recognized. The preclinical laboratory testing against established human tumor cell lines that were maintained in tissue cultures for decades, and against human tumors extricated from their natural habitat and grown in xenografts, may not yield valid results predictive of the viral therapy applied against human tumors growing in their natural environment, the human host. Since the recent discovery of the oncosuppressive efficacy of bacteriophages, the colon could be regarded as the battlefield, where incipient tumor cells and bacteriophages vie for dominance. The inner environment of the colon will be the teaching ground providing new knowledge on the value of the anti-tumor efficacy of phage-induced innate anti-tumor immune reactions. Genetically engineered oncolytic viruses are reviewed next. The molecular biology of viral oncolysis is explained in details. Elaborate efforts are presented to elucidate how gene product proteins of oncolytic viruses switch off the oncogenic cascades of cancer cells. The facts strongly support the conclusion that viral therapy of human cancers will remain in the front lines of modern cancer therapeutics. It may be a

  17. Human dental pulp stem cell is a promising autologous seed cell for bone tissue engineering

    Institute of Scientific and Technical Information of China (English)

    LI Jing-hui; LIU Da-yong; ZHANG Fang-ming; WANG Fan; ZHANG Wen-kui; ZHANG Zhen-ting

    2011-01-01

    Background The seed cell is a core problem in bone tissue engineering research.Recent research indicates that human dental pulp stem cells (hDPSCs) can differentiate into osteoblasts in vitro,which suggests that they may become a new kind of seed cells for bone tissue engineering.The aim of this study was to evaluate the osteogenic differentiation of hDPSCs in vitro and bone-like tissue formation when transplanted with three-dimensional gelatin scaffolds in vivo,and hDPSCs may become appropriate seed cells for bone tissue engineering.Methods We have utilized enzymatic digestion to obtain hDPSCs from dental pulp tissue extracted during orthodontic treatment.After culturing and expansion to three passages,the cells were seeded in 6-well plates or on three-dimensional gelatin scaffolds and cultured in osteogenic medium.After 14 days in culture,the three-dimensional gelatin scaffolds were implanted subcutaneously in nude mice for 4 weeks.In 6-well plate culture,osteogenesis was assessed by alkaline phosphatase staining,Von Kossa staining,and reverse transcription-polymerase chain reaction (RT-PCR) analysis of the osteogenesis-specific genes type I collagen (COL l),bone sialoprotein (BSP),osteocalcin (OCN),RUNX2,and osterix (OSX).In three-dimensional gelatin scaffold culture,X-rays,hematoxylin/eosin staining,and immunohistochemical staining were used to examine bone formation.Results In vitro studies revealed that hDPSCs do possess osteogenic differentiation potential.In vivo studies revealed that hDPSCs seeded on gelatin scaffolds can form bone structures in heterotopic sites of nude mice.Conclusions These findings suggested that hDPSCs may be valuable as seed cells for bone tissue engineering.As a special stem cell source,hDPSCs may blaze a new path for bone tissue engineering.

  18. Targeting human microRNA genes using engineered Tal-effector nucleases (TALENs.

    Directory of Open Access Journals (Sweden)

    Ruozhen Hu

    Full Text Available MicroRNAs (miRNAs have quickly emerged as important regulators of mammalian physiology owing to their precise control over the expression of critical protein coding genes. Despite significant progress in our understanding of how miRNAs function in mice, there remains a fundamental need to be able to target and edit miRNA genes in the human genome. Here, we report a novel approach to disrupting human miRNA genes ex vivo using engineered TAL-effector (TALE proteins to function as nucleases (TALENs that specifically target and disrupt human miRNA genes. We demonstrate that functional TALEN pairs can be designed to enable disruption of miRNA seed regions, or removal of entire hairpin sequences, and use this approach to successfully target several physiologically relevant human miRNAs including miR-155*, miR-155, miR-146a and miR-125b. This technology will allow for a substantially improved capacity to study the regulation and function of miRNAs in human cells, and could be developed into a strategic means by which miRNAs can be targeted therapeutically during human disease.

  19. NRC Reviewer Aid for Evaluating the Human Factors Engineering Aspects of Small Modular Reactors

    Energy Technology Data Exchange (ETDEWEB)

    OHara J. M.; Higgins, J.C.

    2012-01-13

    Small modular reactors (SMRs) are a promising approach to meeting future energy needs. Although the electrical output of an individual SMR is relatively small compared to that of typical commercial nuclear plants, they can be grouped to produce as much energy as a utility demands. Furthermore, SMRs can be used for other purposes, such as producing hydrogen and generating process heat. The design characteristics of many SMRs differ from those of current conventional plants and may require a distinct concept of operations (ConOps). The U.S. Nuclear Regulatory Commission (NRC) conducted research to examine the human factors engineering (HFE) and the operational aspects of SMRs. The research identified thirty potential human-performance issues that should be considered in the NRC's reviews of SMR designs and in future research activities. The purpose of this report is to support NRC HFE reviewers of SMR applications by identifying some of the questions that can be asked of applicants whose designs have characteristics identified in the issues. The questions for each issue were identified and organized based on the review elements and guidance contained in Chapter 18 of the Standard Review Plan (NUREG-0800), and the Human Factors Engineering Program Review Model (NUREG-0711).

  20. Human Factors Engineering (HFE) insights for advanced reactors based upon operating experience

    Energy Technology Data Exchange (ETDEWEB)

    Higgins, J.; Nasta, K.

    1997-01-01

    The NRC Human Factors Engineering Program Review Model (HFE PRM, NUREG-0711) was developed to support a design process review for advanced reactor design certification under 10CFR52. The HFE PRM defines ten fundamental elements of a human factors engineering program. An Operating Experience Review (OER) is one of these elements. The main purpose of an OER is to identify potential safety issues from operating plant experience and ensure that they are addressed in a new design. Broad-based experience reviews have typically been performed in the past by reactor designers. For the HFE PRM the intent is to have a more focussed OER that concentrates on HFE issues or experience that would be relevant to the human-system interface (HSI) design process for new advanced reactors. This document provides a detailed list of HFE-relevant operating experience pertinent to the HSI design process for advanced nuclear power plants. This document is intended to be used by NRC reviewers as part of the HFE PRM review process in determining the completeness of an OER performed by an applicant for advanced reactor design certification. 49 refs.

  1. Towards breaking the silence between the two cultures: Engineering and the other humanities

    Energy Technology Data Exchange (ETDEWEB)

    Prausnitz, John M.

    2003-01-01

    Over the years, I have attended numerous meetings like this one at the Center for the Study of Higher Education. I have noticed that most of the attendees, and certainly the speakers, tend to come from the social sciences or humanities. Only rarely do I see anyone here from Berkeley's College of Chemistry or College of Engineering. I come from the College of Chemistry that includes Berkeley's Department of Chemical Engineering. I mention this background to indicate that my remarks here are necessarily less abstract, less theoretical and less philosophical than those of most previous seminar speakers. My remarks are probably somewhat simplistic because, as a result of my engineering background, I tend to focus less on generalities and principles, giving more attention to possible solutions of limited practical problems. About seven weeks ago, I was invited to attend a conference sponsored by the Berlin Academy of Sciences where ''Sciences'' is not confined to natural sciences but includes also humanities and social sciences. The topic of the Conference was ''Sprachlosigkeit'', a German word that roughly translated means inability to speak. The subtitle was ''Silence Between the Disciplines''. The German universities are worried about the increasing gulf between what is often called ''the two cultures''. This gulf is a problem everywhere, including Berkeley, but it is my impression that it is much worse in Europe than in America. The International Conference in Berlin was attended by some big names including the presidents of the Humboldt University in Berlin, the University of Uppsala in Sweden and the Central European University of Budapest, as well as some distinguished academics from a variety of institutions including Harvard and Stanford, and the presidents of three major funding organizations: The Volkswagen Foundation, The German National Science Foundation and the Max

  2. Enhanced Electrical Integration of Engineered Human Myocardium via Intramyocardial versus Epicardial Delivery in Infarcted Rat Hearts.

    Directory of Open Access Journals (Sweden)

    Kaytlyn A Gerbin

    Full Text Available Cardiac tissue engineering is a promising approach to provide large-scale tissues for transplantation to regenerate the heart after ischemic injury, however, integration with the host myocardium will be required to achieve electromechanical benefits. To test the ability of engineered heart tissues to electrically integrate with the host, 10 million human embryonic stem cell (hESC-derived cardiomyocytes were used to form either scaffold-free tissue patches implanted on the epicardium or micro-tissue particles (~1000 cells/particle delivered by intramyocardial injection into the left ventricular wall of the ischemia/reperfusion injured athymic rat heart. Results were compared to intramyocardial injection of 10 million dispersed hESC-cardiomyocytes. Graft size was not significantly different between treatment groups and correlated inversely with infarct size. After implantation on the epicardial surface, hESC-cardiac tissue patches were electromechanically active, but they beat slowly and were not electrically coupled to the host at 4 weeks based on ex vivo fluorescent imaging of their graft-autonomous GCaMP3 calcium reporter. Histologically, scar tissue physically separated the patch graft and host myocardium. In contrast, following intramyocardial injection of micro-tissue particles and suspended cardiomyocytes, 100% of the grafts detected by fluorescent GCaMP3 imaging were electrically coupled to the host heart at spontaneous rate and could follow host pacing up to a maximum of 300-390 beats per minute (5-6.5 Hz. Gap junctions between intramyocardial graft and host tissue were identified histologically. The extensive coupling and rapid response rate of the human myocardial grafts after intramyocardial delivery suggest electrophysiological adaptation of hESC-derived cardiomyocytes to the rat heart's pacemaking activity. These data support the use of the rat model for studying electromechanical integration of human cardiomyocytes, and they

  3. Comparison of different fabrication techniques for human adipose tissue engineering in severe combined immunodeficient mice.

    Science.gov (United States)

    Frerich, Bernhard; Winter, Karsten; Scheller, Konstanze; Braumann, Ulf-Dietrich

    2012-03-01

    Adipose tissue engineering has been advocated for soft-tissue augmentation and for the treatment of soft tissue defects. The efficacy in terms of persistence of the engineered fat is, however, not yet understood and could depend on the nature of fabrication and application. The high metabolic demand of adipose tissue also points to the problem of vascularization. Endothelial cell (EC) cotransplantation could be a solution. Human adipose tissue-derived stromal cells were seeded on collagen microcarriers and submitted to adipogenic differentiation ("microparticles"). In a first run of experiments, these microparticles were implanted under the skin of severe combined immunodeficient (SCID) mice (n = 45) with and without the addition of human umbilical vein ECs (HUVECs). A group of carriers without any cells served as control. In a second run, adipose tissue constructs were fabricated by embedding microparticles in fibrin matrix with and without the addition of HUVEC, and were also implanted in SCID mice (n = 30). The mice were sacrificed after 12 days, 4 weeks, and 4 months. Mature adipose tissue, fibrous tissue, and acellular regions were quantified on whole-specimen histological sections. The implantation of microparticles showed a better sustainment of tissue volume and a higher degree of mature adipose tissue compared with adipose tissue constructs. Immunohistology proved obviously perfused human tissue-engineered vessels. There was a limited but not significant advantage in EC cotransplantation after 4 weeks in terms of tissue volume. In groups with EC cotransplantation, there were significantly fewer acellular/necrotic areas after 4 weeks and 4 months. In conclusion, the size of the implanted tissue equivalents is a crucial parameter, affecting volume maintenance and the gain of mature adipose tissue. EC cotransplantation leads to functional stable vascular networks connecting in part to the host vasculature and contributing to tissue perfusion; however

  4. Stirling engines. (Latest citations from the Aerospace database). Published Search

    Energy Technology Data Exchange (ETDEWEB)

    1993-09-01

    The bibliography contains citations concerning fuel consumption, engine design and testing, computerized simulation, and lubrication systems relative to the Stirling cycle engine. Solar energy conversion research, thermodynamic efficiency, economics, and utilization for power generation and automobile engines are included. Materials used in Stirling engines are briefly evaluated. (Contains 250 citations and includes a subject term index and title list.)

  5. DEVELOPMENT OF HUMAN FACTORS ENGINEERING GUIDANCE FOR SAFETY EVALUATIONS OF ADVANCED REACTORS.

    Energy Technology Data Exchange (ETDEWEB)

    O' HARA, J.; PERSENSKY, J.; SZABO, A.

    2006-10-01

    Advanced reactors are expected to be based on a concept of operations that is different from what is currently used in today's reactors. Therefore, regulatory staff may need new tools, developed from the best available technical bases, to support licensing evaluations. The areas in which new review guidance may be needed and the efforts underway to address the needs will be discussed. Our preliminary results focus on some of the technical issues to be addressed in three areas for which new guidance may be developed: automation and control, operations under degraded conditions, and new human factors engineering methods and tools.

  6. Enabling Robotic Social Intelligence by Engineering Human Social-Cognitive Mechanisms

    DEFF Research Database (Denmark)

    Wiltshire, Travis; Warta, Samantha F.; Barber, Daniel

    2017-01-01

    for artificial cognitive systems. We discuss a recent integrative perspective of social cognition to provide a systematic theoretical underpinning for computational instantiations of these mechanisms. We highlight several commitments of our approach that we refer to as Engineering Human Social Cognition. We...... then provide a series of recommendations to facilitate the development of the perceptual, motor, and cognitive architecture for this proposed artificial cognitive system in future work. For each recommendation, we highlight their relation to the discussed social-cognitive mechanisms, provide the rationale...

  7. Construction of tissue-engineered cartilage using human placenta-derived stem cells

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Human placenta-derived stem cells (hPDSCs) were isolated by trypsinization and further induced into cartilage cells in vitro.The engineered cartilage was constructed by combining hPDSCs with collagen sponge and the cartilage formation was observed by implantation into nude mice.Results showed that hPDSCs featured mesenchymal stem cells and maintained proliferation in vitro for over 30 passages while remaining undifferentiated.All results indicated that hPDSCs have the potential to differentiate into functional cartilage cells in vitro when combined with collagen sponge,which provided experimental evidence for prospective clinical application.

  8. Human Factors Engineering. A Self-Paced Text, Lessons 6-10,

    Science.gov (United States)

    1981-08-01

    saga of his super chopper. Eager presented to the manager some of the very problems you’ve had to deal with in the last several lessons. Their talk...in the continuing saga (soap opera?) of I. M. Eager. As you recall from the last few lessons, Eager was dreaming that he had de- signed a perfect...importance of some of the things Captain B. Smart had been saying about Human Factors Engineering. As twilight set in, Eager realized that in lis rush to

  9. Bibliography of marine biology in South Africa

    CSIR Research Space (South Africa)

    Darracott, DA

    1980-02-01

    Full Text Available This bibliography was sponsored by the Marine Biology Section of the South African National Committee for Oceanographic Research (SANCOR). It has been attempted to include all publications which appeared before the end of 1977, either in South...

  10. Remotely piloted vehicles; A selective bibliography

    Energy Technology Data Exchange (ETDEWEB)

    Farley, R. [comp.

    1975-12-01

    This report is a bibliography from the International Aerospace Abstracts and the Scientific and Technical Aerospace Abstracts on remotely piloted vehicles. Most of the applications of these RPV`s are military in nature.

  11. Alcohol fuels bibliography, 1901-March 1980

    Energy Technology Data Exchange (ETDEWEB)

    1981-04-01

    This annotated bibliography is subdivided by subjects, as follows: general; feedstocks-general; feedstocks-sugar; feedstocks-starch; feedstocks-cellulose crops and residues; production; coproducts; economics; use as vehicle fuel; government policies; and environmental effects and safety. (MHR)

  12. Geopressured geothermal bibliography. Volume 1 (citation extracts)

    Energy Technology Data Exchange (ETDEWEB)

    Hill, T.R.; Sepehrnoori, K.

    1981-08-01

    This bibliography was compiled by the Center for Energy Studies at The University of Texas at Austin to serve as a tool for researchers in the field of geopressured geothermal energy resources. The bibliography represents citations of papers on geopressured geothermal energy resources over the past eighteen years. Topics covered in the bibliography range from the technical aspects of geopressured geothermal reservoirs to social, environmental, and legal aspects of tapping those reservoirs for their energy resources. The bibliography currently contains more than 750 entries. For quick reference to a given topic, the citations are indexed into five divisions: author, category, conference title, descriptor, and sponsor. These indexes are arranged alphabetically and cross-referenced by page number.

  13. The American Revolution; A Bibliography of Multimedia

    Science.gov (United States)

    Fultz, Norma J.

    1975-01-01

    This bibliography lists several types of multimedia instructional materials useful in teaching the American Revolution for elementary and secondary students. The following types of media are included: audiotapes, films, filmstrips, kits, phonodiscs, pictures, realia, simulations, slides, and transparencies. (JR)

  14. Juvenile Correctional Institutions Library Services: A Bibliography.

    Science.gov (United States)

    McAlister, Annette M.

    This bibliography lists citations for 14 articles, books, and reports concerned with library services in juvenile correctional institutions. A second section lists 21 additional materials on adult correctional libraries which also contain information relevant to the juvenile library. (KP)

  15. White Sturgeon Bibliography, 1985 Final Report.

    Energy Technology Data Exchange (ETDEWEB)

    Fickeisen, Duane H. (Pacific Northwest National Laboratory, Richland, WA)

    1986-03-01

    This bibliography presents citations to the majority of published materials on white sturgeon (Acipenser transmontanus). The purpose was to assist in planning and implementing research on white sturgeon in the Columbia River system. (ACR)

  16. Bibliographie der Werke von Herbert Spencer

    OpenAIRE

    Schmid, Michael (Prof.)

    1991-01-01

    Bibliographie der Werke von Herbert Spencer / bearb., hrsg., mit e. Einl. u. e. Kurzbiogr. vers. von Michael Schmid u. Margit Weihrich. - München : Inst. für Soziologie u. Gesellschaftspolitik, Univ. d. Bundeswehr, 1991. - 159 S.

  17. Teaching Business Communication Skills: A Bibliography.

    Science.gov (United States)

    Sensenbaugh, Roger

    1993-01-01

    Presents annotations of 18 conference papers, books, curriculum guides, and conference proceedings that discuss teaching and assessing business communication skills. Notes that materials in the annotated bibliography were published between 1985 and 1992. (RS)

  18. Author Clustering on Large Bibliographies

    CERN Document Server

    Sterz, Christoph

    2014-01-01

    We analyze and design an algorithm for clustering large sets of authors in Bibliographies. Not considering a distance function for a mutual comparison, but transforming the data into a multidimensional metric space, the algorithm described is similar to locally sensitive hashing. The task lies in the field of Record-Linkage. The algorithm was designed and performed based on the data of the CERN Document Server, consisting out of more than 1.7 million metadata entries and is part of the digital assets-managing-software invenio. Meant as a prototype, the algorithm performs efficiently, clustering all authors on CDS in under 30 minutes. We will discuss extensions improving the recall rate, wich still remains inferior to the currently used clustering-approach.

  19. Bibliographie

    OpenAIRE

    2016-01-01

    Les sources des textes d’Elizabeth Cady Stanton traduits dans l’Anthologie sont indiquées en début d’ouvrage. Ouvrages et articles critiques Anderson Bonnie S., « The lid comes off : international radical feminism and the revolutions of 1848 », NWSA (National Women’s Studies Association) Journal, vol. 10, no 2, été 1998, p. 1-12.— Joyous Greetings : The First International Women’s Movement, 1830-1860, Oxford, Oxford University Press, 2000.— « Early international feminism : the contributions a...

  20. Bibliographie

    OpenAIRE

    2016-01-01

    Contextes Bataillon, Marcel « La librería del estudiante Morlanes », Homenaje a Don Agustín Millares Carlo, Caja Insular de Ahorros, Las Palmas de Gran Canaria, 1975, p. 329-348. Battesti Pélegrin, Jeanne « Nommer les choses : le poète cancioneril par lui-même », B. Hi., XC, 1988, p. 5-25.« La poésie “cancioneril” ou l’anti-autobiographie ? », L’autobiographie dans le monde hispanique, Aix-en-Provence, 1980, p. 95-113. Blanco Aquinaga, Carlos, Rodríguez-Puértolas, Julio y Zavala, Iris M. « Po...

  1. Bibliography

    NARCIS (Netherlands)

    NN,

    1956-01-01

    Adamson, R.S.: The South African species of Aizoaceae. II. Tetragonia. (J. S. Afr. Bot. 21, 1955, 109-154). Key. Akiyama, S.: Carices of the Far Eastern region. Sapporo. 1955. 1-257. t. 1-248. Magnificent monograph; keys & descr. in Jap.; syn. in Engl.

  2. Bibliography

    NARCIS (Netherlands)

    Steenis, van C.G.G.J.

    1977-01-01

    The entries have been split into five categories: a) Algae – b) Fungi & Lichens — c) Bryophytes — d) Pteridophytes — e) Spermatophytes & General subjects. — Books have been marked with an asterisk.

  3. Bibliography

    NARCIS (Netherlands)

    Steenis, van C.G.G.J.

    1979-01-01

    The entries have been split into five categories: a) Algae — b) Fungi & Lichens — c) Bryophytes — d) Pteridophytes — e) Spermatophytes & General subjects. — Books have been marked with an asterisk: *.

  4. Bibliographie

    OpenAIRE

    2013-01-01

    Aguirre Beltrán, Gonzálo, 1953, Formas de gobierno indígena, México, Imprenta Universitaria. Alvarado de Tezozomoc, Hernando de, 1987, Crónica Mexicana, México, Éd. Porrúa, 2 t. Anderson, Arthur J. O. et Dibble, Charles E., 1953-1982, Florentine Codex, Utah, The University of Utah, The School of American Research, 12 vol. Barrera Hernández Abel, 1996, Ritualidad y poder entre los Nahuas de la Montaña de Guerrero, Cultura y derechos de los pueblos indígenas de México, México, Fondo de Culture ...

  5. Bibliographie

    OpenAIRE

    2016-01-01

    ABDOU Souley Ibrou, Monographie de quelques bailleurs de fonds. Procédures de programmation des investissements de l’Etat au Niger, Document multigraphié. ABDOU Souley Ibrou, NATCAP comme instrument de planification et de coordination, document multigraphié non daté, PNUD, Niamey. ALBERTINI Jean-Marie, L’économie en 200 schémas, Les Editions de l’Atelier, (Points d’appui), Paris, 1994. ALINSKY Saul, Manuel de l’animateur social, (Points), Seuil, Paris, 1978. BADIE Bertrand et SMOUTS Marie-Cla...

  6. Bibliographie

    OpenAIRE

    2015-01-01

    1/ Relations Internationales, transferts, diffusion et circulation de modèles politiques Ouvrages sur le Mexique Aldecoa Francisco, Keating Michael, Paradiplomacy in Action : The Foreign Relaitons of Subnational Governements, Londres, Frank Cass, 1999. Atlani-Duault Laëtitia, « Les ONG à l’heure de la bonne gouvernance », Autrepart, n° 35, 2005, p. 317. Badie Bertrand, L’État importé : occidentalisation de l’ordre politique, Paris, Fayard, 1992. Beck Ulrich, Pouvoir et contrepouvoir à l’heure...

  7. Bibliographie

    OpenAIRE

    2014-01-01

    1/ Ouvrages généraux sur le Porfiriat Ouvrages sur le Mexique Florescano Enrique (dir.) (1988), Atlas histórico de México, México, Siglo Veintiuno. González Navarro Moises (1957), El Porfiriato y la vida social, t. IV, Ed. Hermes. Guerra François-Xavier (1979), « Pouvoir central et pouvoirs régionaux au Mexique à la veille de la Révolution », Revue française d’Outre-mer, t. LXVI, n° 244-245. Guerra François-Xavier et Lempérière Annick (coord.) (1998), Los espacios públicos en Iberoamérica : a...

  8. Bibliographie

    OpenAIRE

    2014-01-01

    Vasco Mariz - « Villa-Lobos » - Seghers, Paris, 1967. Lisa Peppercorn - « Villa-Lobos, Leben und Werk des brasilianischen Komponisten », Zurich, 1972. Larousse de la Musique - Rubriques « Brésil », « Villa-Lobos » et notices traitant des principales œuvres à titres. Paris, 1982. Pierre Vidal - « Heitor Villa-Lobos et l’âme brésilienne », « Diapason » numéro 322, décembre 1986. Luis Paulo Horta - « Villa-Lobos, ediçao do centenario », Musée Villa-Lobos, Rio de Janeiro, 1987. Anna-Stella Schic ...

  9. Bibliographie

    OpenAIRE

    2015-01-01

    Abu-Lughod J., 1987 Intemational Joumal of Middle East Studies, 19-2, p. 155-176. Abboud-Haggar S., 1999 El tratado jurídico de al-Tafrī’ de Ibn aI-Ğallāb, manuscrito aIjamiado de Almonacid de la Sierra, Zaragoza, 2 vols. Abboud-Haggar S., a paraitre « Ibn Sahl », Diccionario de Autores y Obras andalusíes, Granada. Abdel-Rahim Muddathir, 1982 « Instituciones juridicas », La ciudad islámica (R.B. Serjeant éd.), Barce1ona, p.49-61. Abdesselem Abou Bekr, 1935 Dictionnaire arabe-français des term...

  10. Bibliographie

    OpenAIRE

    2013-01-01

    Bardet G., L'urbanisme, PUF, coll. « Que sais-je ? », Paris, 4e édition 1959 (1re édition 1945). Benevolo L., Histoire de l'architecture moderne, tome 1, Bordas, Paris, 1987. Benevolo L., Histoire de l'architecture moderne, tome 2, Bordas, Paris, 1988. Bourgey A. et Phares J., « Les bidonvilles de l'agglomération de Beyrouth », dans Revue de géographie de Lyon, 1973, p. 107-139. Bourgey A., « La Guerre et ses Conséquences Géographiques au Liban », dans Annales de Géographie n° 521, XCIVe anné...

  11. Bibliography

    OpenAIRE

    Tiercelin, Claudine

    2014-01-01

    Abbreviations CCWJ = R. A. Putnam (ed.), 1997, The Cambridge Companion to William James. James, ERE = James, 1912, Essays in Radical Empiricism. James, MT = James, 1909/1975, The Meaning of Truth. James, P = James, 1907/1975, Pragmatism. James, PP = James, 1981, The Principles of Psychology. James, PU = James, 1977, A Pluralistic Universe. James, WB = James, 1979, The Will to Believe. Peirce, CP = Peirce, 1931-1958, The Complete Papers of C.S. Peirce. Peirce, NEM = Peirce, 1976, The New Eleme...

  12. Bibliography

    NARCIS (Netherlands)

    NN,

    1997-01-01

    BERNARD, S. & R. DE KONINCK. 1996. The retreat of the forest in Southeast Asia: a cartographic assessment. Singapore J. Trop. Geogr. 17: 1-14, illus. — 2 maps of 1970 and 1990 compared; ‘retreat’ euphemism for ‘destruction’. BHAT, R.B. 1996. Leaf architecture and its taxonomic significance in the St

  13. Bibliographie

    OpenAIRE

    2014-01-01

    Les abréviations, sauf celles explicitées ci-dessous, sont conformes à l’Année philologique (http%20:/%20www.%20annee-philologique.%20com/aph%20/), au Guide de l’épigraphiste (Bérard Fr., Feisseil D., Petitmengin P., Rousset D. et Sève M. (dir.), Paris, 2000 3) et à la Checklist en ligne (Oates J. F., Bagnall R. S., Clackson S. J., O’Brien A. A., Sosin J. D., Wilfong T. G. et Worp Kl. A., Checklist of Greek, Latin, Demotic and Coptic Papyri, Ostraca and Tablets, http :// scriptorium. lib. duk...

  14. Bibliography

    NARCIS (Netherlands)

    NN,

    1961-01-01

    Agnihothrudu, V.: A new genus of the helicosporous Basidiomycetes (from North-East India) (Fungi) (Trans. Brit. Myc. Soc. 44, 1961, 51-54, 1 fig.). Ahmad, S.: Further contributions to the Fungi of West Pakistan 1 (Biologia 6, 1960, 117-136, 17 fig.).

  15. Bibliography

    OpenAIRE

    2016-01-01

    “4th Asia Pacific Consultation on Refugee Rights Concludes,” Asia Pacific Refugee Rights Network, 30 August 2012, http://www.aprrn.info/1/index.php/news/1234th-asia-pacific-consultation-on-refugee-rights-concludes “6년만에 기업인으로 돌아온 문국현 ‘ 한솔섬유 히든챔피언 만들겠다,” Hankyoreh, 4 July 2013, http://www.hani.co.kr/arti/economy/economy_general/594451.html “140 Chain of Disasters,” in Open Scenarios Repository (Alexandria: United States Institute for Defense Analyses), http://openscenarios.ida.org/docs/OpenSce...

  16. Bibliographie

    OpenAIRE

    2015-01-01

    Sources ESCAP. Statistical Yearbook for Asia and the Pacific 1973. Bangkok, United Nations, Economic Commission for Asia and the Pacific, 1974, 447 pages. ESCAP. Statistical Yearbook for Asia and the Pacific 1980. Bangkok, United Nations, Economic Commission for Asia and the Pacific, 1981, 526 pages. FAO. Production Yearbook 1973. Roma, Food and Agricultural Organization of the United Nations, vol. 27, 1974, 523 pages. FAO. Production Yearbook 1976. Roma, Food and Agricultural Organization of...

  17. Bibliography

    NARCIS (Netherlands)

    NN,

    1976-01-01

    The entries have been split into five categories: a) Algae – b) Fungi & Lichens — c) Bryophytes — d) Pteridophytes — e) Spermatophytes & General subjects. — Books have been marked with an asterisk.

  18. Bibliographie

    OpenAIRE

    2014-01-01

    Aguilar Medina José Iñigo, 1980, El hombre y la urbe, la ciudad de Oaxaca, Mexico, Sep/Inah. Aguilar Urbina Luisa, 1988, « Reconstrucción de vecindades en Tepito », dans, Cisneros Sosa Armando (coord.), Rescate y reconstruccion del centro de la ciudad de México, Mexico, Uam-Iztapalapa, p. 66-95. Aguilera Gomez Manuel, 1988, « politica de vivienda popular : Nuevas experiencias para su reformulación », conférence présentée, Seminario : la modernización de las ciudades en México, Mexico, Unam. A...

  19. Bibliography

    NARCIS (Netherlands)

    NN,

    1998-01-01

    CAPESIUS, I. & M. STECH. 1997. Molecular relationships within mosses based on 18S rRNA gene sequences. Nova Hedw. 64: 525-533, illus. — Sphagnidae sister to Bryidae; Funariales, Polytrichales distinct; Orthotrichum not part of paraphyletic Hypnobryanae. CHIANG, T.Y. 1998. Taxonomic revision of Andre

  20. Bibliography

    NARCIS (Netherlands)

    NN,

    1962-01-01

    Abeywickrama, B. A.: A provisional check list of the flowering plants of Ceylon (Ceylon J. Sc., Biol. Sec. 2, 1959, 119- 240). Ahti, T.: Taxonomic studies on reindeer lichens (Cladonia, subg. Cladina) (Ann. Bot. Soc. Zool. Bot Fenn. Vanamo 32¹, 1961, 1-160, many fig.). Also map of C. mitis, p. 121.

  1. Bibliographie

    OpenAIRE

    2016-01-01

    Abi-Ezzi Nathalie, The Double in the Fiction of R.L. Stevenson, Wilkie Collins and Daphne du Maurier, Oxford, Peter Lang, 2003. Adam Ian & Tiffin Helen (dir.), Past the Last Post : Theorizing Post-Colonialism and Post-Modernism, Ian London, Harvester Wheatsheaf, 1991. Artaud Antonin, Le Théâtre et son double, Paris, Gallimard, 1944. Ashcroft Bill, On Post-Colonial Futures : Transformations of Colonial Cultures, New York, Continuum, 2001. Ashcroft Bill, Griffiths Gareth & Tiffin Helen, Th...

  2. Bibliographie

    OpenAIRE

    2012-01-01

    Ackerley Chris J., Gontarski Stanley E., The Grove Companion to Samuel Beckett, New York, Grove Press, 2004. Albright Daniel, Beckett and Aesthetics, Cambridge, Cambridge University Press, 2003. Aristote, Poétique, Paris, Librairie générale française (Le livre de poche), 1990. Arrowsmith William, Introduction à The Birds, in Four Plays by Aristophanes : The Birds ; The Clouds ; The Frogs ; Lysistrata, New York, Meridian, 1994. Artaud Antonin, Le théâtre et son double, Paris, Gallimard, 1964. ...

  3. Bibliography

    NARCIS (Netherlands)

    NN,

    1961-01-01

    Agnihothrudu, V.: A new genus of the helicosporous Basidiomycetes (from North-East India) (Fungi) (Trans. Brit. Myc. Soc. 44, 1961, 51-54, 1 fig.). Ahmad, S.: Further contributions to the Fungi of West Pakistan 1 (Biologia 6, 1960, 117-136, 17 fig.).

  4. Bibliographie

    OpenAIRE

    2013-01-01

    Ouvrages Abdel Fattah N., Al wahj wa l-qanar [Le visage et le voile], Le Caire, Al-Sechat, 1995. Abdel Rahman H., Uses and Gratifications of Satellite TV in Egypt, thèse de Masters, université américaine du Caire, 1998. Abou-Haidar E., Libéralisme et Capitalisme d’État en Égypte, Paris, L’Harmattan, 2000. Achilleas Ph., La Télévision par satellite, aspects juridiques internationaux, Paris, Monchrestien, 1997. Al-Hadidi M. et A., Al-fidyû kasît, anmât mushâhadatihi wa ta’thîratuhu [Vidéo-Casse...

  5. Bibliographie

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    Alexander Robert Jackson, « The Latin American Aprista Parties », Political Quarterly, n° 20, 1949, p. 236-247 Álvarez Junco José, Gonzalez Luis (comp.), El populismo en España y América, Madrid, Catriel, 1994. Álvarez Junco José, El emperador del Paralelo. Lerroux et la demagogia populista, Madrid, Alianza, 1990. Ansart Pierre, La gestion des passions politiques, Lausanne, L’Âge d’Homme, 1983. Baciu Sergio, « Un Continente en Busca de Una Doctrina », Journal of Inter-American Studies, n° 2, ...

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    Anderson Benedict, L’imaginaire national. Réflexions sur l’origine et l’essor du nationalisme, Paris, La Découverte, 2002. Arguedas José Maria, El Sexto, Barcelone, Laia, 1974. Avilés Marco,Día de visita, Confesiones de mujeres desde el penal Santa Mónica, Lima, Aguilar, 2007. Azaola E., Prisiones para mujeres : un enfoque de género, México, Programa interdisciplinario de Estudios de la Mujer, 1998. Bejar Héctor, « Las cárceles en el Perú ¿rehabilitación o castigo ? » in « Visión del Perú », ...

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    Benzécri Jean-Paul et al., L’analyse des données, Paris, Dunod, 1973, vol. 1 : La taxinomie, vol. 2 : Correspondances. Constitue la référence pour l’analyse des correspondances mais se situe, pour la partie théorique, à un niveau élevé de compétences mathématiques. Cibois Philippe, « Le PEM, pourcentage de l’écart maximum : un indice de liaison entre modalités d’un tableau de contingence », Bulletin de méthodologie sociologique, 1993, no 40, p. 43-63. Cibois Philippe, « Les pièges de l’analys...

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    Vasco Mariz - « Villa-Lobos » - Seghers, Paris, 1967. Lisa Peppercorn - « Villa-Lobos, Leben und Werk des brasilianischen Komponisten », Zurich, 1972. Larousse de la Musique - Rubriques « Brésil », « Villa-Lobos » et notices traitant des principales œuvres à titres. Paris, 1982. Pierre Vidal - « Heitor Villa-Lobos et l’âme brésilienne », « Diapason » numéro 322, décembre 1986. Luis Paulo Horta - « Villa-Lobos, ediçao do centenario », Musée Villa-Lobos, Rio de Janeiro, 1987. Anna-Stella Schic ...

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    Alabarces Pablo, Crónicas del aguante, futbol, violencia y política, Buenos Aires, capital intelectual, 2004. Alonso Guillermo, « La reforma del seguro social argentino », in Viviane Brachet-Marquez Salud publica y regimenes de pensiones en la era neo-liberal, Mexico, El Colegio de Mexico, 2007, p. 45-101. Alonso Guillermo, La reforma del sistema de pensiones argentino, in Viviane Brachet-Marquez Salud publica y regimenes de pensiones en la era neo-liberal, Mexico, El Colegio de Mexico, 2007,...

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    Sources primaires Chine Almanac of China ’s Foreign Economic Relations and Trade, The Editorial Board of the Almanac of China’s Foreign Economic Relations and Trade, China Economics Publishing House Economie Information & Agency, Beijing,1993-2001. Puis Yearbook of China’s foreign economic relations and trade 2001,Beijing, China Foreign Economie Relations and Trade Publishing House,2002-2003. « Bishkek Statement », Xinhua Domestic Service dans FBIS-CHI-1999-0826, 25August 1999. « Déclaration ...

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    I - Ouvrages généraux : traités, manuels, précis et dictionnaires Arnaud (A-J), (dir.) Dictionnaire encyclopédique de théorie et de sociologie du droit, LGDJ, 2e éd., 1993. Aubert (J-L), Introduction au droit et thèmes fondamentaux du droit civil, A. Colin, 9e éd., 2002. Aubry (C) et Rau (C), Cours de droit civil français, t. VI, 4e éd., 1869-1876. Balle (F) et Cohen-Tanugi (L), (dir.) Dictionnaire du web, Dalloz, 2001. Batiffol (H), Problèmes de base de philosophie du droit, LGDJ, 1979. Bati...

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    Abodunrin, Yinka. 1981. The role of architecture in the prevention of crime: A suburban experience. In: Social Science and Social Policy in Nigeria. O. Sanda, ed. Nigerian Institute of Social and Economic Research (NISER), Ibadan. Aderinto, A. 1994. Student unrest and urban violence in Nigeria, pp. 233-238. In: Urban Management and Urban Violence in Africa (Vol. 2). I.O. Albert, J. Adisa, T. Agbola and G. Hérault, eds. Institut Français de Recherche en Afrique (IFRA), Ibadan. Adisa, J. 1994. ...

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    I. Romans John Banville Birchwood, Londres, Minerva, 1973. Doctor Copernicus [1976], Londres, Picador, 1999. Kepler, Londres, Minerva, 1981 ; Kepler, trad. fr. Michèle Albaret-Maatsch, Paris, Flammarion, 1992. The Newton Letter, Londres, Picador, 1982 ; La Lettre de Newton, Interlude, trad. fr. Michèle Albaret-Maatsch, Paris, Flammarion, 1996. Mefisto, Londres, Picador, 1986. The Book of Evidence, Londres, Minerva, 1989 ; Le Livre des aveux, trad. fr. Michèle Albaret-Maatsch, Arles, Actes Sud,...

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    ANGELIAUME, A. (1996) Ruissellement, érosion et qualité des eaux en terre de grande culture. Étude de cas en Laonnois et Soissonnais, Thèse de doctorat, Université de Lille I, 488 pages + 220 pages annexes. ANGELIAUME, A., WICHEREK, S., DACHARRY, M. (1994) Ruissellement, érosion et qualité des eaux en terre de grande culture, Hydrologie continentale, Publication de l’ORSTOM, 9, 2, p. 107-122. AOUNI, L. (1995) Étude de l’occupation/utilisation du sol et de son impact sur les terres agricoles d...

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    AKIYAMA, H., T. YAMAGUCHI & M. MOHAMED. 1999. A new variety of Polytrichadelphus archboldii Bartr. (Polytrichaceae Musci) from Mt. Kinabalu, Sabah. Acta Phytotax. Geobot. 49: 135-141, illus. ARTS, T. 1998. A revision of the moss genus Gymnostomiella Fleisch. J. Bryol. 20:411- 427, illus. — 6 taxa, 1 comb nov; key; synonymy, descriptions, notes.

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    AKIYAMA, H., T. YAMAGUCHI & M. MOHAMED. 1999. A new variety of Polytrichadelphus archboldii Bartr. (Polytrichaceae Musci) from Mt. Kinabalu, Sabah. Acta Phytotax. Geobot. 49: 135-141, illus. ARTS, T. 1998. A revision of the moss genus Gymnostomiella Fleisch. J. Bryol. 20:411- 427, illus. — 6 taxa, 1

  17. Bibliographie

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    Ackerman Ada, 2005, « Ivan le Terrible ou la Sacralisation de Staline (1943-1945) », Théorème, no 8. Ackerman Ada, 2010, « l’Icône orthodoxe dans les films russes et soviétiques ou la réactivation du sacré », CinémAction (Croyances et sacré au cinéma), no134. Agel Henri, 1953, le Cinéma et le Sacré, Paris : Éd. du Cerf. Akira Kurosawa : dessins, 2008, Paris : Paris-Musées. Albéra François (éd.), 1996, les Formalistes russes et le Cinéma, Paris : Nathan. Albéra François, 2005, l’Avant-garde au...

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    Abajo las murallas ! 150 anys de l’enderroc de les muralles de Barcelona, Ajuntament de Barcelona, 2005. Agulhon, Maurice, Marianne au pouvoir. L’imagerie et la symbolique républicaine de 1880 à nos jours, Paris, Flammarion, 1985. Ainaud, Josep Maria ; Jardí, Enric, Prat de la Riba, home de govern, Barcelona, Ariel, 1973. Aizpuru, Mikel, « Sobre la astenia del nacionalismo español a finales del siglo xix y comienzos del xx », Historia Contemporánea, 23, 2001 (II), p. 811-849. Albareda, Joaqui...

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    Œuvres de Sa‘dallah Wannous سعد الله ونوس، ١٩٩٦ الأعمال الكاملة، ٣ مجلدات، دشق، الأهالي. ١٩٩٧ الأيام المخمورة، دمشق، الأهالي. ١٩٩٧ عن الذاكرة و الموت، دمشق، الأهالي. ٢٠٠٤ الحياةأبدا، بيروت، دار الآداب. Wannous, Saadallah, 1996 Miniatures suivi de Rituel pour une métamorphose, traduction française de Marie Elias, Hanan Kassab Hassan et de Rania Samara, Arles, Sinbad-Actes Sud, Papiers. 2001 Une mort éphémère, traduction de Rania Samara, Arles, Sindbad-Actes Sud. Ouvrages et articles en langue ...

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    OpenAIRE

    2016-01-01

    Textual sources أبو القاسم محمد بن علي بن حوقل، دي غوج، ١٨٧٣، كتاب صورة الأرض. الحسن بن أحمد الهمدني، محمد بن علي الأكوع ،١٩٩٠، صفة جزيرة العرب، صنعاء: مكتبة الإرشاد. الحسن بن أحمد الهمدني، يوسف محمد عبد الله،٢٠٠٣، كتاب الجوهرتين العتيقتبن المائعتبن من الصفراء والبيضاء، صنعاء: مكتبة الأرشاد. الرازي، حسين بن عبد الله العمري ،١٩٧٤، كتاب تاريخ مدينة صنعاء، بيروت: دار الفكر المعاصر - دمشق: دار الفكر. إدريس عماد الدين، أيمان فوأد سيد ،٢٠٠٢، عيون الأخبار، لوندون. عمارة، محمد بن علي الأكوع،١٩٨٥، تار...

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    The entries have been split into five categories: a) Algae — b) Fungi & Lichenes — c) Bryophytes — d) Pteridophytes — e) Spermatophytes & General subjects. — Books have been marked with an asterisk.

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    Steenis, van C.G.G.J.

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    Steenis, van C.G.G.J.

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    The entries have been split into five categories: a) Algae — b) Fungi & Lichens — c) Bryophytes — d) Pteridophytes — e) Spermatophytes 8 General subjects. — Books have been marked with an asterisk.

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    Steenis, van C.G.G.J.

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    The entries have been split into five categories: (a) Algae — (b) Fungi & Lichens — (c) Bryophytes — (d) Pteridophytes — (e) Spermatophytes & General subjects. — Books have been marked with an asterisk.

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    Steenis, van C.G.G.J.

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    The entries have been split into five categories: (a) Algae — (b) Fungi & Lichens — (c) Bryophytes — (d) Pteridophytes — (e) Spermatophytes & General subjects. — Books have been marked with an asterisk.

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    Steenis, van C.G.G.J.

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    Steenis, van C.G.G.J.

    1977-01-01

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    Œuvres étudiées Hoffmann E. T. A., « Le Marchand de sable » (1815), in Tableaux nocturnes, Paris, Imprimerie nationale, 1999. James H., « The Turn of the Screw » (1898), in The Aspern Papers and The Turn of the Screw, Harmondsworth, Penguin, 1986. Le Fanu S., Carmilla (1872), in Best Ghost Stories, New York, Dover, 1964. Rice A., Interview with the Vampire, (1976), London, Warner Books, 1996. Shelley M., Frankenstein, (1831), Harmondsworth, Penguin, 1992. Stevenson R. L., « The Strange Case o...

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    [Abbate, 2000] Abbate, Janet. 2000, Inventing the Internet, Cambridge MA, MIT Press. [Adler, 1997] Adler, Emanuel. “Seizing the Middle Ground: Constructivism in World Politics”, European Journal of International Relations 3, 31963, 1997. [Aguiton & Cardon, 2007a] Aguiton, Christophe & Cardon, Dominique. « De la cooptation à l’agglutination. Culture participative et formes organisationnelles des forums sociaux », in Neveu, Catherine, Cultures et pratiques participatives. Perspectives comparati...

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    Alabarces Pablo, Crónicas del aguante, futbol, violencia y política, Buenos Aires, capital intelectual, 2004. Alonso Guillermo, « La reforma del seguro social argentino », in Viviane Brachet-Marquez Salud publica y regimenes de pensiones en la era neo-liberal, Mexico, El Colegio de Mexico, 2007, p. 45-101. Alonso Guillermo, La reforma del sistema de pensiones argentino, in Viviane Brachet-Marquez Salud publica y regimenes de pensiones en la era neo-liberal, Mexico, El Colegio de Mexico, 2007,...

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    Chambe S., Lambert A., Tarlabaşı, CD Rom, OUI, IFEA, İstanbul, 2001. Çakır R., Direniş ve İtaat (Résistance et soumission), İstanbul : Metis Yayınları, 2000. Göle N., « Islam in Public : New Visibilities and New Imaginaries », Public Culture, n° 14, 2002, pp. 173-190. Göle N., The Forbidden Modern : Civilization and Veiling, University of Michigan Press, 1996. Sönmez M., Grafiklerle 1990’larda İstanbul, İstanbul, İstanbul Büyükşehir Belediyesi Kültür İşleri Başkanlığı Yayınları, 1994. Sönmez ...

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    SOMMAIRE I - 1 Archives et Manuscrits de Boulainvilliers - Archives et manuscrits relatifs à la biographie de Boulainvilliers - Manuscrits de Boulainvilliers A) Manuscrits attribués à Boulainvilliers B) Attributions erronées I-2 Sources imprimées de Boulainvilliers - Oeuvres politiques sur l’histoire de France - Oeuvres philosophiques, astrologiques et portant sur l’histoire de la religion (utilisées) I-3 Ouvrages sur Boulainvilliers I-4 Articles sur Boulainvilliers II - 1 Imprimés et manuscr...

  19. A Path to Planetary Protection Requirements for Human Exploration: A Literature Review and Systems Engineering Approach

    Science.gov (United States)

    Johnson, James E.; Conley, Cassie; Siegel, Bette

    2015-01-01

    As systems, technologies, and plans for the human exploration of Mars and other destinations beyond low Earth orbit begin to coalesce, it is imperative that frequent and early consideration is given to how planetary protection practices and policy will be upheld. While the development of formal planetary protection requirements for future human space systems and operations may still be a few years from fruition, guidance to appropriately influence mission and system design will be needed soon to avoid costly design and operational changes. The path to constructing such requirements is a journey that espouses key systems engineering practices of understanding shared goals, objectives and concerns, identifying key stakeholders, and iterating a draft requirement set to gain community consensus. This paper traces through each of these practices, beginning with a literature review of nearly three decades of publications addressing planetary protection concerns with respect to human exploration. Key goals, objectives and concerns, particularly with respect to notional requirements, required studies and research, and technology development needs have been compiled and categorized to provide a current 'state of knowledge'. This information, combined with the identification of key stakeholders in upholding planetary protection concerns for human missions, has yielded a draft requirement set that might feed future iteration among space system designers, exploration scientists, and the mission operations community. Combining the information collected with a proposed forward path will hopefully yield a mutually agreeable set of timely, verifiable, and practical requirements for human space exploration that will uphold international commitment to planetary protection.

  20. Human engineered heart tissue as a versatile tool in basic research and preclinical toxicology.

    Directory of Open Access Journals (Sweden)

    Sebastian Schaaf

    Full Text Available Human embryonic stem cell (hESC progenies hold great promise as surrogates for human primary cells, particularly if the latter are not available as in the case of cardiomyocytes. However, high content experimental platforms are lacking that allow the function of hESC-derived cardiomyocytes to be studied under relatively physiological and standardized conditions. Here we describe a simple and robust protocol for the generation of fibrin-based human engineered heart tissue (hEHT in a 24-well format using an unselected population of differentiated human embryonic stem cells containing 30-40% α-actinin-positive cardiac myocytes. Human EHTs started to show coherent contractions 5-10 days after casting, reached regular (mean 0.5 Hz and strong (mean 100 µN contractions for up to 8 weeks. They displayed a dense network of longitudinally oriented, interconnected and cross-striated cardiomyocytes. Spontaneous hEHT contractions were analyzed by automated video-optical recording and showed chronotropic responses to calcium and the β-adrenergic agonist isoprenaline. The proarrhythmic compounds E-4031, quinidine, procainamide, cisapride, and sertindole exerted robust, concentration-dependent and reversible decreases in relaxation velocity and irregular beating at concentrations that recapitulate findings in hERG channel assays. In conclusion this study establishes hEHT as a simple in vitro model for heart research.

  1. On recent advances in human engineering Provocative trends in embryology, genetics, and regenerative medicine.

    Science.gov (United States)

    Anton, Roman

    2016-01-01

    Advances in embryology, genetics, and regenerative medicine regularly attract attention from scientists, scholars, journalists, and policymakers, yet implications of these advances may be broader than commonly supposed. Laboratories culturing human embryos, editing human genes, and creating human-animal chimeras have been working along lines that are now becoming intertwined. Embryogenic methods are weaving traditional in vivo and in vitro distinctions into a new "in vivitro" (in life in glass) fabric. These and other methods known to be in use or thought to be in development promise soon to bring society to startling choices and discomfiting predicaments, all in a global effort to supply reliably rejuvenating stem cells, to grow immunologically non-provocative replacement organs, and to prevent, treat, cure, or even someday eradicate diseases having genetic or epigenetic mechanisms. With humanity's human-engineering era now begun, procedural prohibitions, funding restrictions, institutional controls, and transparency rules are proving ineffective, and business incentives are migrating into the most basic life-sciences inquiries, wherein lie huge biomedical potentials and bioethical risks. Rights, health, and heritage are coming into play with bioethical presumptions and formal protections urgently needing reassessment.

  2. Human-factors engineering for smart transport: design support for car drivers and train traffic controllers.

    Science.gov (United States)

    Lenior, Dick; Janssen, Wiel; Neerincx, Mark; Schreibers, Kirsten

    2006-07-01

    The theme Smart Transport can be described as adequate human-system symbiosis to realize effective, efficient and human-friendly transport of goods and information. This paper addresses how to attune automation to human (cognitive) capacities (e.g. to take care of information uncertainty, operator trust and mutual man-machine adaptations). An introduction to smart transport is presented, including examples of best practice for engineering human factors in the vehicle ergonomics and train traffic control domain. The examples are representative of an ongoing trend in automation and they show how the human role changes from controller to supervisor. Section 2 focuses on the car driver and systems that support, or sometimes even take over, critical parts of the driving task. Due to the diversity of driver ability, driving context and dependence between driver and context factors, there is a need for personalised, adaptive and integrated support. Systematic research is needed to establish sound systems. Section 3 focuses on the train dispatcher support systems that predict train movements, detect potential conflicts and show the dispatcher the possibilities available to solve the detected problems. Via thorough analysis of both the process to be controlled and the dispatcher's tasks and cognitive needs, support functions were developed as part of an already very complex supervision and control system. The two examples, although from a different field, both show the need for further development in cognitive modelling as well as for the value of sound ergonomics task analysis in design practice.

  3. In vitro cytokeratin expression profiling of human oral mucosa substitutes developed by tissue engineering.

    Science.gov (United States)

    Garzon, Ingrid; Serrato, Deyanira; Roda, Olga; Del Carmen Sanchez-Quevedo, Maria; Gonzales-Jaranay, Maximino; Moreu, Gerardo; Nieto-Aguilar, Renato; Alaminos, Miguel; Campos, Antonio

    2009-10-01

    In this work we performed a study of cytokeratin (CK) expression profiling on human artificial oral mucosa developed in vitro by tissue engineering at different stages of maturation (from immature to well-developed stages) at the protein and mRNA levels. Human artificial oral mucosa was generated in the laboratory using fibrin-agarose biomaterials. As controls, we used human native normal oral mucosa and embryonic oral tissues. Our results demonstrated that human embryonic oral tissues tended to express CK8 and CK19. In contrast, monolayered bioengineered oral mucosa did not show any CK expression by immunohistochemistry, whereas bilayered and multilayered artificial oral mucosa showed several markers of stratified epithelia, but did not express CK10. These results suggest that the CK expression pattern is strongly dependent on the maturation state of the artificial tissues and that the CK expression profile of our model of artificial oral mucosa was partially similar to that of the non-keratinized human adult oral mucosa. However, the expression of CK8 by the artificial oral mucosa suggests that these samples correspond to an early stage of development while kept in vitro.

  4. Martian Surface Boundary Layer Characterization: Enabling Environmental Data for Science, Engineering and Human Exploration

    Science.gov (United States)

    England, C.

    2000-01-01

    For human or large robotic exploration of Mars, engineering devices such as power sources will be utilized that interact closely with the Martian environment. Heat sources for power production, for example, will use the low ambient temperature for efficient heat rejection. The Martian ambient, however, is highly variable, and will have a first order influence on the efficiency and operation of all large-scale equipment. Diurnal changes in temperature, for example, can vary the theoretical efficiency of power production by 15% and affect the choice of equipment, working fluids, and operating parameters. As part of the Mars Exploration program, missions must acquire the environmental data needed for design, operation and maintenance of engineering equipment including the transportation devices. The information should focus on the variability of the environment, and on the differences among locations including latitudes, altitudes, and seasons. This paper outlines some of the WHY's, WHAT's and WHERE's of the needed data, as well as some examples of how this data will be used. Environmental data for engineering design should be considered a priority in Mars Exploration planning. The Mars Thermal Environment Radiator Characterization (MTERC), and Dust Accumulation and Removal Technology (DART) experiments planned for early Mars landers are examples of information needed for even small robotic missions. Large missions will require proportionately more accurate data that encompass larger samples of the Martian surface conditions. In achieving this goal, the Mars Exploration program will also acquire primary data needed for understanding Martian weather, surface evolution, and ground-atmosphere interrelationships.

  5. Martian Surface Boundary Layer Characterization: Enabling Environmental Data for Science, Engineering and Human Exploration

    Science.gov (United States)

    England, C.

    2000-01-01

    For human or large robotic exploration of Mars, engineering devices such as power sources will be utilized that interact closely with the Martian environment. Heat sources for power production, for example, will use the low ambient temperature for efficient heat rejection. The Martian ambient, however, is highly variable, and will have a first order influence on the efficiency and operation of all large-scale equipment. Diurnal changes in temperature, for example, can vary the theoretical efficiency of power production by 15% and affect the choice of equipment, working fluids, and operating parameters. As part of the Mars Exploration program, missions must acquire the environmental data needed for design, operation and maintenance of engineering equipment including the transportation devices. The information should focus on the variability of the environment, and on the differences among locations including latitudes, altitudes, and seasons. This paper outlines some of the WHY's, WHAT's and WHERE's of the needed data, as well as some examples of how this data will be used. Environmental data for engineering design should be considered a priority in Mars Exploration planning. The Mars Thermal Environment Radiator Characterization (MTERC), and Dust Accumulation and Removal Technology (DART) experiments planned for early Mars landers are examples of information needed for even small robotic missions. Large missions will require proportionately more accurate data that encompass larger samples of the Martian surface conditions. In achieving this goal, the Mars Exploration program will also acquire primary data needed for understanding Martian weather, surface evolution, and ground-atmosphere interrelationships.

  6. Human umbilical cord stem cell encapsulation in novel macroporous and injectable fibrin for muscle tissue engineering

    Science.gov (United States)

    Liu, Jun; Xu, Hockin H.K.; Zhou, Hongzhi; Weir, Michael D.; Chen, Qianming; Trotman, Carroll Ann

    2012-01-01

    There has been little research on the seeding of human umbilical cord mesenchymal stem cells (hUCMSCs) in three-dimensional scaffolds for muscle tissue engineering. The objectives of this study were: (i) to seed hUCMSCs in a fibrin hydrogel containing fast-degradable microbeads (dMBs) to create macropores to enhance cell viability; and (ii) to investigate the encapsulated cell proliferation and myogenic differentiation for muscle tissue engineering. Mass fractions of 0–80% of dMBs were tested, and 35% of dMBs in fibrin was shown to avoid fibrin shrinkage while creating macropores and promoting cell viability. This construct was referred to as “dMB35”. Fibrin without dMBs was termed “dMB0”. Microbead degradation created macropores in fibrin and improved cell viability. The percentage of live cells in dMB35 reached 91% at 16 days, higher than the 81% in dMB0 (p muscle tissue engineering applications. PMID:22902812

  7. Human amniotic fluid derived cells can competently substitute dermal fibroblasts in a tissue-engineered dermo-epidermal skin analog

    NARCIS (Netherlands)

    Hartmann-Fritsch, Fabienne; Hosper, Nynke; Luginbuehl, Joachim; Biedermann, Thomas; Reichmann, Ernst; Meuli, Martin

    2013-01-01

    Human amniotic fluid comprises cells with high differentiation capacity, thus representing a potential cell source for skin tissue engineering. In this experimental study, we investigated the ability of human amniotic fluid derived cells to substitute dermal fibroblasts and support epidermis formati

  8. Mechanical and biochemical mapping of human auricular cartilage for reliable assessment of tissue-engineered constructs.

    Science.gov (United States)

    Nimeskern, Luc; Pleumeekers, Mieke M; Pawson, Duncan J; Koevoet, Wendy L M; Lehtoviita, Iina; Soyka, Michael B; Röösli, Christof; Holzmann, David; van Osch, Gerjo J V M; Müller, Ralph; Stok, Kathryn S

    2015-07-16

    It is key for successful auricular (AUR) cartilage tissue-engineering (TE) to ensure that the engineered cartilage mimics the mechanics of the native tissue. This study provides a spatial map of the mechanical and biochemical properties of human auricular cartilage, thus establishing a benchmark for the evaluation of functional competency in AUR cartilage TE. Stress-relaxation indentation (instantaneous modulus, Ein; maximum stress, σmax; equilibrium modulus, Eeq; relaxation half-life time, t1/2; thickness, h) and biochemical parameters (content of DNA; sulfated-glycosaminoglycan, sGAG; hydroxyproline, HYP; elastin, ELN) of fresh human AUR cartilage were evaluated. Samples were categorized into age groups and according to their harvesting region in the human auricle (for AUR cartilage only). AUR cartilage displayed significantly lower Ein, σmax, Eeq, sGAG content; and significantly higher t1/2, and DNA content than NAS cartilage. Large amounts of ELN were measured in AUR cartilage (>15% ELN content per sample wet mass). No effect of gender was observed for either auricular or nasoseptal samples. For auricular samples, significant differences between age groups for h, sGAG and HYP, and significant regional variations for Ein, σmax, Eeq, t1/2, h, DNA and sGAG were measured. However, only low correlations between mechanical and biochemical parameters were seen (Rbiochemical map of human auricular cartilage. Regional variations in mechanical and biochemical properties were demonstrated in the auricle. This finding highlights the importance of focusing future research on efforts to produce cartilage grafts with spatially tunable mechanics. Copyright © 2015 Elsevier Ltd. All rights reserved.

  9. Continual improvement: A bibliography with indexes, 1992-1993

    Science.gov (United States)

    1994-01-01

    This bibliography lists 606 references to reports and journal articles entered into the NASA Scientific and Technical Information Database during 1992 to 1993. Topics cover the philosophy and history of Continual Improvement (CI), basic approaches and strategies for implementation, and lessons learned from public and private sector models. Entries are arranged according to the following categories: Leadership for Quality, Information and Analysis, Strategic Planning for CI, Human Resources Utilization, Management of Process Quality, Supplier Quality, Assessing Results, Customer Focus and Satisfaction, TQM Tools and Philosophies, and Applications. Indexes include subject, personal author, corporate source, contract number, report number, and accession number.

  10. Chondroprotective supplementation promotes the mechanical properties of injectable scaffold for human nucleus pulposus tissue engineering.

    Science.gov (United States)

    Foss, Berit L; Maxwell, Thomas W; Deng, Ying

    2014-01-01

    A result of intervertebral disc (IVD) degeneration, the nucleus pulposus (NP) is no longer able to withstand applied load leading to pain and disability. The objective of this study is to fabricate a tissue-engineered injectable scaffold with chondroprotective supplementation in vitro to improve the mechanical properties of a degenerative NP. Tissue-engineered scaffolds were fabricated using different concentrations of alginate and calcium chloride and mechanically evaluated. Fabrication conditions were based on structural and mechanical resemblance to the native NP. Chondroprotective supplementation, glucosamine (GCSN) and chondroitin sulfate (CS), were added to scaffolds at concentrations of 0:0µg/mL (0:0-S), 125:100µg/mL (125:100-S), 250:200µg/mL (250:200-S), and 500:400µg/mL (500:400-S), GCSN and CS, respectively. Scaffolds were used to fabricate tissue-engineered constructs through encapsulation of human nucleus pulposus cells (HNPCs). The tissue-engineered constructs were collected at days 1, 14, and 28 for biochemical and biomechanical evaluations. Confocal microscopy showed HNPC viability and rounded morphology over the 28 day period. MTT analysis resulted in significant increases in cell proliferation for each group. Collagen type II ELISA quantification and compressive aggregate moduli (HA) showed increasing trends for both 250:200-S and the 500:400-S groups on Day 28 with significantly greater HA compared to 0:0-S group. Glycosaminoglycan and water content decreased for all groups. Results indicate the increased mechanical properties of the 250:200-S and the 500:400-S was due to production of a functional matrix. This study demonstrated potential for a chondroprotective supplemented injectable scaffold to restore biomechanical function of a degenerative disc through the production of a mechanically functional matrix.

  11. Comparative Human Health Impact Assessment of Engineered Nanomaterials in the Framework of Life Cycle Assessment.

    Science.gov (United States)

    Fransman, Wouter; Buist, Harrie; Kuijpers, Eelco; Walser, Tobias; Meyer, David; Zondervan-van den Beuken, Esther; Westerhout, Joost; Klein Entink, Rinke H; Brouwer, Derk H

    2017-07-01

    For safe innovation, knowledge on potential human health impacts is essential. Ideally, these impacts are considered within a larger life-cycle-based context to support sustainable development of new applications and products. A methodological framework that accounts for human health impacts caused by inhalation of engineered nanomaterials (ENMs) in an indoor air environment has been previously developed. The objectives of this study are as follows: (i) evaluate the feasibility of applying the CF framework for NP exposure in the workplace based on currently available data; and (ii) supplement any resulting knowledge gaps with methods and data from the life cycle approach and human risk assessment (LICARA) project to develop a modified case-specific version of the framework that will enable near-term inclusion of NP human health impacts in life cycle assessment (LCA) using a case study involving nanoscale titanium dioxide (nanoTiO2 ). The intent is to enhance typical LCA with elements of regulatory risk assessment, including its more detailed measure of uncertainty. The proof-of-principle demonstration of the framework highlighted the lack of available data for both the workplace emissions and human health effects of ENMs that is needed to calculate generalizable characterization factors using common human health impact assessment practices in LCA. The alternative approach of using intake fractions derived from workplace air concentration measurements and effect factors based on best-available toxicity data supported the current case-by-case approach for assessing the human health life cycle impacts of ENMs. Ultimately, the proposed framework and calculations demonstrate the potential utility of integrating elements of risk assessment with LCA for ENMs once the data are available. © 2016 Society for Risk Analysis.

  12. 3D Bioprinting Human Chondrocytes with Nanocellulose-Alginate Bioink for Cartilage Tissue Engineering Applications.

    Science.gov (United States)

    Markstedt, Kajsa; Mantas, Athanasios; Tournier, Ivan; Martínez Ávila, Héctor; Hägg, Daniel; Gatenholm, Paul

    2015-05-11

    The introduction of 3D bioprinting is expected to revolutionize the field of tissue engineering and regenerative medicine. The 3D bioprinter is able to dispense materials while moving in X, Y, and Z directions, which enables the engineering of complex structures from the bottom up. In this study, a bioink that combines the outstanding shear thinning properties of nanofibrillated cellulose (NFC) with the fast cross-linking ability of alginate was formulated for the 3D bioprinting of living soft tissue with cells. Printability was evaluated with concern to printer parameters and shape fidelity. The shear thinning behavior of the tested bioinks enabled printing of both 2D gridlike structures as well as 3D constructs. Furthermore, anatomically shaped cartilage structures, such as a human ear and sheep meniscus, were 3D printed using MRI and CT images as blueprints. Human chondrocytes bioprinted in the noncytotoxic, nanocellulose-based bioink exhibited a cell viability of 73% and 86% after 1 and 7 days of 3D culture, respectively. On the basis of these results, we can conclude that the nanocellulose-based bioink is a suitable hydrogel for 3D bioprinting with living cells. This study demonstrates the potential use of nanocellulose for 3D bioprinting of living tissues and organs.

  13. Engineering the oxygen sensing regulation results in an enhanced recombinant human hemoglobin production by Saccharomyces cerevisiae.

    Science.gov (United States)

    Martínez, José L; Liu, Lifang; Petranovic, Dina; Nielsen, Jens

    2015-01-01

    Efficient production of appropriate oxygen carriers for transfusions (blood substitutes or artificial blood) has been pursued for many decades, and to date several strategies have been used, from synthetic polymers to cell-free hemoglobin carriers. The recent advances in the field of metabolic engineering also allowed the generation of different genetically modified organisms for the production of recombinant human hemoglobin. Several studies have showed very promising results using the bacterium Escherichia coli as a production platform, reporting hemoglobin titers above 5% of the total cell protein content. However, there are still certain limitations regarding the protein stability and functionality of the recombinant hemoglobin produced in bacterial systems. In order to overcome these limitations, yeast systems have been proposed as the eukaryal alternative. We recently reported the generation of a set of plasmids to produce functional human hemoglobin in Saccharomyces cerevisiae, with final titers of active hemoglobin exceeding 4% of the total cell protein. In this study, we propose a strategy for further engineering S. cerevisiae by altering the oxygen sensing pathway by deleting the transcription factor HAP1, which resulted in an increase of the final recombinant active hemoglobin titer exceeding 7% of the total cellular protein.

  14. Clinical Application of Human Mesenchymal Stromal Cells for Bone Tissue Engineering

    Directory of Open Access Journals (Sweden)

    Anindita Chatterjea

    2010-01-01

    Full Text Available The gold standard in the repair of bony defects is autologous bone grafting, even though it has drawbacks in terms of availability and morbidity at the harvesting site. Bone-tissue engineering, in which osteogenic cells and scaffolds are combined, is considered as a potential bone graft substitute strategy. Proof-of-principle for bone tissue engineering using mesenchymal stromal cells (MSCs has been demonstrated in various animal models. In addition, 7 human clinical studies have so far been conducted. Because the experimental design and evaluation parameters of the studies are rather heterogeneous, it is difficult to draw conclusive evidence on the performance of one approach over the other. However, it seems that bone apposition by the grafted MSCs in these studies is observed but not sufficient to bridge large bone defects. In this paper, we discuss the published human clinical studies performed so far for bone-tissue regeneration, using culture-expanded, nongenetically modified MSCs from various sources and extract from it points of consideration for future clinical studies.

  15. Creating capillary networks within human engineered tissues: impact of adipocytes and their secretory products.

    Science.gov (United States)

    Aubin, Kim; Vincent, Caroline; Proulx, Maryse; Mayrand, Dominique; Fradette, Julie

    2015-01-01

    The development of tissue-engineered substitutes of substantial volume is closely associated with the need to ensure rapid vascularization upon grafting. Strategies promoting angiogenesis include the in vitro formation of capillary-like networks within engineered substitutes. We generated both connective and adipose tissues based on a cell sheet technology using human adipose-derived stromal cells. This study evaluates the morphology and extent of the capillary networks that developed upon seeding of human microvascular endothelial cells during tissue production. We posited that adipocyte presence/secretory products could modulate the resulting capillary network when compared to connective substitutes. Analyses including confocal imaging of CD31-labeled capillary-like networks indicated slight differences in their morphological appearance. However, the total volume occupied by the networks as well as the frequency distribution of the structure's volumes were similar between connective and adipose tissues. The average diameter of the capillary structures tended to be 20% higher in reconstructed adipose tissues. Quantification of pro-angiogenic molecules in conditioned media showed greater amounts of leptin (15×), angiopoietin-1 (3.4×) and HGF (1.7×) secreted from adipose than connective tissues at the time of endothelial cell seeding. However, this difference was attenuated during the following coculture period in endothelial cell-containing media, correlating with the minor differences noted between the networks. Taken together, we developed a protocol allowing reconstruction of both connective and adipose tissues featuring well-developed capillary networks in vitro. We performed a detailed characterization of the network architecture within engineered tissues that is relevant for graft assessment before implantation as well as for in vitro screening of angiogenic modulators using three-dimensional models. Copyright © 2014 Acta Materialia Inc. Published by

  16. Cartilage tissue engineering of nasal septal chondrocyte-macroaggregates in human demineralized bone matrix.

    Science.gov (United States)

    Liese, Juliane; Marzahn, Ulrike; El Sayed, Karym; Pruss, Axel; Haisch, Andreas; Stoelzel, Katharina

    2013-06-01

    Tissue Engineering is an important method for generating cartilage tissue with isolated autologous cells and the support of biomaterials. In contrast to various gel-like biomaterials, human demineralized bone matrix (DBM) guarantees some biomechanical stability for an application in biomechanically loaded regions. The present study combined for the first time the method of seeding chondrocyte-macroaggregates in DBM for the purpose of cartilage tissue engineering. After isolating human nasal chondrocytes and creating a three-dimensional macroaggregate arrangement, the DBM was cultivated in vitro with the macroaggregates. The interaction of the cells within the DBM was analyzed with respect to cell differentiation and the inhibitory effects of chondrocyte proliferation. In contrast to chondrocyte-macroaggregates in the cell-DBM constructs, morphologically modified cells expressing type I collagen dominated. The redifferentiation of chondrocytes, characterized by the expression of type II collagen, was only found in low amounts in the cell-DBM constructs. Furthermore, caspase 3, a marker for apoptosis, was detected in the chondrocyte-DBM constructs. In another experimental setting, the vitality of chondrocytes as related to culture time and the amount of DBM was analyzed with the BrdU assay. Higher amounts of DBM tended to result in significantly higher proliferation rates of the cells within the first 48 h. After 96 h, the vitality decreased in a dose-dependent fashion. In conclusion, this study provides the proof of concept of chondrocyte-macroaggregates with DBM as an interesting method for the tissue engineering of cartilage. The as-yet insufficient redifferentiation of the chondrocytes and the sporadic initiation of apoptosis will require further investigations.

  17. Flexor tendon tissue engineering: acellularization of human flexor tendons with preservation of biomechanical properties and biocompatibility.

    Science.gov (United States)

    Pridgen, Brian C; Woon, Colin Y L; Kim, Maxwell; Thorfinn, Johan; Lindsey, Derek; Pham, Hung; Chang, James

    2011-08-01

    Acellular human tendons are a candidate scaffold for tissue engineering flexor tendons of the hand. This study compared acellularization methods and their compatibility with allogeneic human cells. Human flexor tendons were pretreated with 0.1% ethylenediaminetetracetic acid (EDTA) for 4  h followed by 24  h treatments of 1% Triton X-100, 1% tri(n-butyl)phosphate, or 0.1% or 1% sodium dodecyl sulfate (SDS) in 0.1% EDTA. Outcomes were assessed histologically by hematoxylin and eosin and SYTO green fluorescent nucleic acid stains and biochemically by a QIAGEN DNeasy kit, Sircol collagen assay, and 1,9 dimethylmethylene blue glycosaminoglycan assay. Mechanical data were collected using a Materials Testing System to pull to failure tendons acellularized with 0.1% SDS. Acellularized tendons were re-seeded in a suspension of human dermal fibroblasts. Attachment of viable cells to acellularized tendon was assessed biochemically by a cell viability assay and histologically by a live/dead stain. Data are reported as mean±standard deviation. Compared with the DNA content of fresh tendons (551±212  ng DNA/mg tendon), only SDS treatments significantly decreased DNA content (1% SDS [202.8±37.4  ng DNA/mg dry weight tendon]; 0.1% SDS [189±104  ng DNA/mg tendon]). These findings were confirmed by histology. There was no decrease in glycosaminoglycans or collagen following acellularization with SDS. There was no difference in the ultimate tensile stress (55.3±19.2 [fresh] vs. 51.5±6.9 [0.1% SDS] MPa). Re-seeded tendons demonstrated attachment of viable cells to the tendon surface using a viability assay and histology. Human flexor tendons were acellularized with 0.1% SDS in 0.1% EDTA for 24  h with preservation of mechanical properties. Preservation of collagen and glycoaminoglycans and re-seeding with human cells suggest that this scaffold is biocompatible. This will provide a promising scaffold for future human flexor tendon tissue engineering studies to

  18. Annotated Bibliography of the Personnel Research Division Reports (1973-1975).

    Science.gov (United States)

    Magness, P. J., Ed.

    An annotated bibliography of 60 technical reports and publications on research conducted by the Personnel Research Division of the Air Force Human Resources Laboratory (AFHRL) is presented. Research covers the identification and measurement of individual aptitude, motivation, morale, attitude, and environment factors. Keyword and personal author…

  19. Mass Communication in India: An Annotated Bibliography. Asian Mass Communication Bibliography Series 2.

    Science.gov (United States)

    Asian Mass Communication Research and Information Centre, Singapore.

    This bibliography lists and describes published and unpublished material written in English that relates to mass communications in India, from 1945 to 1973. The items are grouped into 21 sections: bibliography and reference material; communication theory and research methods; communication (general); media development and characteristics;…

  20. Literatura Chicana. A Comprehensive Bibliography (1980-June, 1984). Working Bibliography Series No. 1.

    Science.gov (United States)

    Trujillo, Roberto G.; And Others

    The 197-item bibliography on Chicano literature published from 1980 through June 1984 is presented in 10 sections: poetry (77 entries), novel (25 entries), short fiction (13 entries), theatre (7 entries), literary criticism (16 entries), anthology (24 entries), unpublished dissertations (13 entries), bibliographies and encyclopedia (6 entries),…

  1. Sex, Death and Military Might: An Introduction to the REMF Bibliography and the REMF Bibliography.

    Science.gov (United States)

    Willson, David A.

    This bibliography provides a chronological panorama of Vietnam War era mass market paperbacks. In an introduction, the bibliography also identifies the major visual themes used on the paperback covers to market the books. It shows the enormous variety as well as the great sameness of the Vietnam market paperback, 1960-1989, a market which saw…

  2. Literatura Chicana. A Comprehensive Bibliography (1980-June, 1984). Working Bibliography Series No. 1.

    Science.gov (United States)

    Trujillo, Roberto G.; And Others

    The 197-item bibliography on Chicano literature published from 1980 through June 1984 is presented in 10 sections: poetry (77 entries), novel (25 entries), short fiction (13 entries), theatre (7 entries), literary criticism (16 entries), anthology (24 entries), unpublished dissertations (13 entries), bibliographies and encyclopedia (6 entries),…

  3. Community Education in Canada. An Annotated Bibliography. OISE Bibliography Series No. 2.

    Science.gov (United States)

    Corman, Linda, Comp.

    This bibliography of 219 numbered entries is limited to Canadian works. However, section 4, "Bibliographies," will provide leads to useful information from the United States. Section 1, "Community Schools/Community Education," includes the more comprehensive works and material about particular Canadian community schools,…

  4. Human-factors engineering control-room design review/audit: Waterford 3 SES Generating Station, Louisiana Power and Light Company

    Energy Technology Data Exchange (ETDEWEB)

    Savage, J.W.

    1983-03-10

    A human factors engineering design review/audit of the Waterford-3 control room was performed at the site on May 10 through May 13, 1982. The report was prepared on the basis of the HFEB's review of the applicant's Preliminary Human Engineering Discrepancy (PHED) report and the human factors engineering design review performed at the site. This design review was carried out by a team from the Human Factors Engineering Branch, Division of Human Factors Safety. The review team was assisted by consultants from Lawrence Livermore National Laboratory (University of California), Livermore, California.

  5. Heart research advances using database search engines, Human Protein Atlas and the Sydney Heart Bank.

    Science.gov (United States)

    Li, Amy; Estigoy, Colleen; Raftery, Mark; Cameron, Darryl; Odeberg, Jacob; Pontén, Fredrik; Lal, Sean; Dos Remedios, Cristobal G

    2013-10-01

    This Methodological Review is intended as a guide for research students who may have just discovered a human "novel" cardiac protein, but it may also help hard-pressed reviewers of journal submissions on a "novel" protein reported in an animal model of human heart failure. Whether you are an expert or not, you may know little or nothing about this particular protein of interest. In this review we provide a strategic guide on how to proceed. We ask: How do you discover what has been published (even in an abstract or research report) about this protein? Everyone knows how to undertake literature searches using PubMed and Medline but these are usually encyclopaedic, often producing long lists of papers, most of which are either irrelevant or only vaguely relevant to your query. Relatively few will be aware of more advanced search engines such as Google Scholar and even fewer will know about Quertle. Next, we provide a strategy for discovering if your "novel" protein is expressed in the normal, healthy human heart, and if it is, we show you how to investigate its subcellular location. This can usually be achieved by visiting the website "Human Protein Atlas" without doing a single experiment. Finally, we provide a pathway to discovering if your protein of interest changes its expression level with heart failure/disease or with ageing. Crown Copyright © 2013. Published by Elsevier B.V. All rights reserved.

  6. Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery.

    Science.gov (United States)

    Lin, Steven; Staahl, Brett T; Alla, Ravi K; Doudna, Jennifer A

    2014-12-15

    The CRISPR/Cas9 system is a robust genome editing technology that works in human cells, animals and plants based on the RNA-programmed DNA cleaving activity of the Cas9 enzyme. Building on previous work (Jinek et al., 2013), we show here that new genetic information can be introduced site-specifically and with high efficiency by homology-directed repair (HDR) of Cas9-induced site-specific double-strand DNA breaks using timed delivery of Cas9-guide RNA ribonucleoprotein (RNP) complexes. Cas9 RNP-mediated HDR in HEK293T, human primary neonatal fibroblast and human embryonic stem cells was increased dramatically relative to experiments in unsynchronized cells, with rates of HDR up to 38% observed in HEK293T cells. Sequencing of on- and potential off-target sites showed that editing occurred with high fidelity, while cell mortality was minimized. This approach provides a simple and highly effective strategy for enhancing site-specific genome engineering in both transformed and primary human cells.

  7. Bibliography of clinical research in malaysia: methods and brief results.

    Science.gov (United States)

    Teng, C L; Zuhanariah, M N; Ng, C S; Goh, C C

    2014-08-01

    This article describes the methodology of this bibliography. A search was conducted on the following: (1) bibliographic databases (PubMed, Scopus, and other databases) using search terms that maximize the retrieval of Malaysian publications; (2) Individual journal search of Malaysian healthrelated journals; (3) A targeted search of Google and Google Scholar; (4) Searching of Malaysian institutional repositories; (5) Searching of Ministry of Health and Clinical Research Centre website. The publication years were limited to 2000- 2013. The citations were imported or manually entered into bibliographic software Refworks. After removing duplicates, and correcting data entry errors, PubMed's Medical Subject Headings (MeSH terms) were added. Clinical research is coded using the definition "patient-oriented-research or research conducted with human subjects (or on material of human origin) for which the investigator directly interacts with the human subjects at some point during the study." A bibliography of citations [n=2056] that fit the criteria of clinical research in Malaysia in selected topics within five domains was generated: Cancers [589], Cardiovascular diseases [432], Infections [795], Injuries [142], and Mental Health [582]. This is done by retrieving citations with the appropriate MESH terms, as follow: For cancers (Breast Neoplasms; Colorectal Neoplasms; Uterine Cervical Neoplasms), for cardiovascular diseases (Coronary Disease; Hypertension; Stroke), for infections (Dengue; Enterovirus Infections, HIV Infections; Malaria; Nipah Virus; Tuberculosis), for injuries (Accidents, Occupational; Accidents, Traffic; Child Abuse; Occupational Injuries), for mental health (Depression; Depressive Disorder; Depressive Disorder, Major; Drug Users; Psychotic Disorders; Suicide; Suicide, Attempted; Suicidal Ideation; Substance- Related Disorders).

  8. Human factors engineering in healthcare systems: the problem of human error and accident management.

    Science.gov (United States)

    Cacciabue, P C; Vella, G

    2010-04-01

    This paper discusses some crucial issues associated with the exploitation of data and information about health care for the improvement of patient safety. In particular, the issues of human factors and safety management are analysed in relation to exploitation of reports about non-conformity events and field observations. A methodology for integrating field observation and theoretical approaches for safety studies is described. Two sample cases are discussed in detail: the first one makes reference to the use of data collected in the aviation domain and shows how these can be utilised to define hazard and risk; the second one concerns a typical ethnographic study in a large hospital structure for the identification of most relevant areas of intervention. The results show that, if national authorities find a way to harmonise and formalize critical aspects, such as the severity of standard events, it is possible to estimate risk and define auditing needs, well before the occurrence of serious incidents, and to indicate practical ways forward for improving safety standards.

  9. Human amniotic fluid derived cells can competently substitute dermal fibroblasts in a tissue-engineered dermo-epidermal skin analog

    OpenAIRE

    2013-01-01

    PURPOSE: Human amniotic fluid comprises cells with high differentiation capacity, thus representing a potential cell source for skin tissue engineering. In this experimental study, we investigated the ability of human amniotic fluid derived cells to substitute dermal fibroblasts and support epidermis formation and stratification in a humanized animal model. METHODS: Dermo-epidermal skin grafts with either amniocytes or with fibroblasts in the dermis were compared in a rat model. Full-thicknes...

  10. Advanced human-system interface design review guideline. Evaluation procedures and guidelines for human factors engineering reviews

    Energy Technology Data Exchange (ETDEWEB)

    O`Hara, J.M.; Brown, W.S. [Brookhaven National Lab., Upton, NY (United States); Baker, C.C.; Welch, D.L.; Granda, T.M.; Vingelis, P.J. [Carlow International Inc., Falls Church, VA (United States)

    1994-07-01

    Advanced control rooms will use advanced human-system interface (HSI) technologies that may have significant implications for plant safety in that they will affect the operator`s overall role in the system, the method of information presentation, and the ways in which operators interact with the system. The U.S. Nuclear Regulatory Commission (NRC) reviews the HSI aspects of control rooms to ensure that they are designed to good human factors engineering principles and that operator performance and reliability are appropriately supported to protect public health and safety. The principal guidance available to the NRC, however, was developed more than ten years ago, well before these technological changes. Accordingly, the human factors guidance needs to be updated to serve as the basis for NRC review of these advanced designs. The purpose of this project was to develop a general approach to advanced HSI review and the human factors guidelines to support. NRC safety reviews of advanced systems. This two-volume report provides the results of the project. Volume I describes the development of the Advanced HSI Design Review Guideline (DRG) including (1) its theoretical and technical foundation, (2) a general model for the review of advanced HSIs, (3) guideline development in both hard-copy and computer-based versions, and (4) the tests and evaluations performed to develop and validate the DRG. Volume I also includes a discussion of the gaps in available guidance and a methodology for addressing them. Volume 2 provides the guidelines to be used for advanced HSI review and the procedures for their use.

  11. Functional characterisation of an engineered multidomain human P450 2E1 by molecular Lego.

    Science.gov (United States)

    Fairhead, Michael; Giannini, Silva; Gillam, Elizabeth M J; Gilardi, Gianfranco

    2005-12-01

    The human cytochrome P450s constitute an important family of monooxygenase enzymes that carry out essential roles in the metabolism of endogenous compounds and foreign chemicals. We present here results of a fusion between a human P450 enzyme and a bacterial reductase that for the first time is shown does not require the addition of lipids or detergents to achieve wild-type-like activities. The fusion enzyme, P450 2E1-BMR, contains the N-terminally modified residues 22-493 of the human P450 2E1 fused at the C-terminus to residues 473-1049 of the P450 BM3 reductase (BMR). The P450 2E1-BMR enzyme is active, self-sufficient and presents the typical marker activities of the native human P450 2E1: the hydroxylation of p-nitrophenol (KM=1.84+/-0.09 mM and kcat of 2.98+/-0.04 nmol of p-nitrocatechol formed per minute per nanomole of P450) and chlorzoxazone (KM=0.65+/-0.08 mM and kcat of 0.95+/-0.10 nmol of 6-hydroxychlorzoxazone formed per minute per nanomole of P450). A 3D model of human P450 2E1 was generated to rationalise the functional data and to allow an analysis of the surface potentials. The distribution of charges on the model of P450 2E1 compared with that of the FMN domain of BMR provides the ground for the understanding of the interaction between the fused domains. The results point the way to successfully engineer a variety of catalytically self-sufficient human P450 enzymes for drug metabolism studies in solution.

  12. Engineering Cartilage

    Science.gov (United States)

    ... Research Matters NIH Research Matters March 3, 2014 Engineering Cartilage Artistic rendering of human stem cells on ... situations has been a major goal in tissue engineering. Cartilage contains water, collagen, proteoglycans, and chondrocytes. Collagens ...

  13. A Novel Human Tissue-Engineered 3-D Functional Vascularized Cardiac Muscle Construct

    Science.gov (United States)

    Valarmathi, Mani T.; Fuseler, John W.; Davis, Jeffrey M.; Price, Robert L.

    2017-01-01

    Organ tissue engineering, including cardiovascular tissues, has been an area of intense investigation. The major challenge to these approaches has been the inability to vascularize and perfuse the in vitro engineered tissue constructs. Attempts to provide oxygen and nutrients to the cells contained in the biomaterial constructs have had varying degrees of success. The aim of this current study is to develop a three-dimensional (3-D) model of vascularized cardiac tissue to examine the concurrent temporal and spatial regulation of cardiomyogenesis in the context of postnatal de novo vasculogenesis during stem cell cardiac regeneration. In order to achieve the above aim, we have developed an in vitro 3-D functional vascularized cardiac muscle construct using human induced pluripotent stem cell-derived embryonic cardiac myocytes (hiPSC-ECMs) and human mesenchymal stem cells (hMSCs). First, to generate the prevascularized scaffold, human cardiac microvascular endothelial cells (hCMVECs) and hMSCs were co-cultured onto a 3-D collagen cell carrier (CCC) for 7 days under vasculogenic culture conditions. In this milieu, hCMVECs/hMSCs underwent maturation, differentiation, and morphogenesis characteristic of microvessels, and formed extensive plexuses of vascular networks. Next, the hiPSC-ECMs and hMSCs were co-cultured onto this generated prevascularized CCCs for further 7 or 14 days in myogenic culture conditions. Finally, the vascular and cardiac phenotypic inductions were analyzed at the morphological, immunological, biochemical, molecular, and functional levels. Expression and functional analyses of the differentiated cells revealed neo-angiogenesis and neo-cardiomyogenesis. Thus, our unique 3-D co-culture system provided us the apt in vitro functional vascularized 3-D cardiac patch that can be utilized for cellular cardiomyoplasty. PMID:28194397

  14. Creation of a Large Adipose Tissue Construct in Humans Using a Tissue-engineering Chamber: A Step Forward in the Clinical Application of Soft Tissue Engineering.

    Science.gov (United States)

    Morrison, Wayne A; Marre, Diego; Grinsell, Damien; Batty, Andrew; Trost, Nicholas; O'Connor, Andrea J

    2016-04-01

    Tissue engineering is currently exploring new and exciting avenues for the repair of soft tissue and organ defects. Adipose tissue engineering using the tissue engineering chamber (TEC) model has yielded promising results in animals; however, to date, there have been no reports on the use of this device in humans. Five female post mastectomy patients ranging from 35 to 49years old were recruited and a pedicled thoracodorsal artery perforator fat flap ranging from 6 to 50ml was harvested, transposed onto the chest wall and covered by an acrylic perforated dome-shaped chamber ranging from 140 to 350cm(3). Magnetic resonance evaluation was performed at three and six months after chamber implantation. Chambers were removed at six months and samples were obtained for histological analysis. In one patient, newly formed tissue to a volume of 210ml was generated inside the chamber. One patient was unable to complete the trial and the other three failed to develop significant enlargement of the original fat flap, which, at the time of chamber explantation, was encased in a thick fibrous capsule. Our study provides evidence that generation of large well-vascularized tissue engineered constructs using the TEC is feasible in humans.

  15. Bibliography of the family Braconidae (Hymenoptera: Ichneumonoidea) (1964-2003)

    NARCIS (Netherlands)

    Ghahari, H.; Yu, D.S.; Achterberg, van C.

    2006-01-01

    A bibliography of the family Braconidae/Hymenoptera: Ichneumonidae is given for the period 1964-2003. It is an addition to Shenefelt's bibliography (1965), which covers the period 1785-1963. In total 10,436 references are listed.

  16. Generation and genetic engineering of human induced pluripotent stem cells using designed zinc finger nucleases.

    Science.gov (United States)

    Ramalingam, Sivaprakash; London, Viktoriya; Kandavelou, Karthikeyan; Cebotaru, Liudmila; Guggino, William; Civin, Curt; Chandrasegaran, Srinivasan

    2013-02-15

    Zinc finger nucleases (ZFNs) have become powerful tools to deliver a targeted double-strand break at a pre-determined chromosomal locus in order to insert an exogenous transgene by homology-directed repair. ZFN-mediated gene targeting was used to generate both single-allele chemokine (C-C motif) receptor 5 (CCR5)-modified human induced pluripotent stem cells (hiPSCs) and biallele CCR5-modified hiPSCs from human lung fibroblasts (IMR90 cells) and human primary cord blood mononuclear cells (CBMNCs) by site-specific insertion of stem cell transcription factor genes flanked by LoxP sites into the endogenous CCR5 locus. The Oct4 and Sox2 reprogramming factors, in combination with valproic acid, induced reprogramming of human lung fibroblasts to form CCR5-modified hiPSCs, while 5 factors, Oct4/Sox2/Klf4/Lin28/Nanog, induced reprogramming of CBMNCs. Subsequent Cre recombinase treatment of the CCR5-modified IMR90 hiPSCs resulted in the removal of the Oct4 and Sox2 transgenes. Further genetic engineering of the single-allele CCR5-modified IMR90 hiPSCs was achieved by site-specific addition of the large CFTR transcription unit to the remaining CCR5 wild-type allele, using CCR5-specific ZFNs and a donor construct containing tdTomato and CFTR transgenes flanked by CCR5 homology arms. CFTR was expressed efficiently from the endogenous CCR5 locus of the CCR5-modified tdTomato/CFTR hiPSCs. These results suggest that it might be feasible to use ZFN-evoked strategies to (1) generate precisely targeted genetically well-defined patient-specific hiPSCs, and (2) then to reshape their function by targeted addition and expression of therapeutic genes from the CCR5 chromosomal locus for autologous cell-based transgene-correction therapy to treat various recessive monogenic human diseases in the future.

  17. Osteogenic Differentiation Capacity of In Vitro Cultured Human Skeletal Muscle for Expedited Bone Tissue Engineering

    Directory of Open Access Journals (Sweden)

    Chunlei Miao

    2017-01-01

    Full Text Available Expedited bone tissue engineering employs the biological stimuli to harness the intrinsic regenerative potential of skeletal muscle to trigger the reparative process in situ to improve or replace biological functions. When genetically modified with adenovirus mediated BMP2 gene transfer, muscle biopsies from animals have demonstrated success in regenerating bone within rat bony defects. However, it is uncertain whether the human adult skeletal muscle displays an osteogenic potential in vitro when a suitable biological trigger is applied. In present study, human skeletal muscle cultured in a standard osteogenic medium supplemented with dexamethasone demonstrated significant increase in alkaline phosphatase activity approximately 24-fold over control at 2-week time point. More interestingly, measurement of mRNA levels revealed the dramatic results for osteoblast transcripts of alkaline phosphatase, bone sialoproteins, transcription factor CBFA1, collagen type I, and osteocalcin. Calcified mineral deposits were demonstrated on superficial layers of muscle discs after an extended 8-week osteogenic induction. Taken together, these are the first data supporting human skeletal muscle tissue as a promising potential target for expedited bone regeneration, which of the technologies is a valuable method for tissue repair, being not only effective but also inexpensive and clinically expeditious.

  18. Three-dimensional computer-aided human factors engineering analysis of a grafting robot.

    Science.gov (United States)

    Chiu, Y C; Chen, S; Wu, G J; Lin, Y H

    2012-07-01

    The objective of this research was to conduct a human factors engineering analysis of a grafting robot design using computer-aided 3D simulation technology. A prototype tubing-type grafting robot for fruits and vegetables was the subject of a series of case studies. To facilitate the incorporation of human models into the operating environment of the grafting robot, I-DEAS graphic software was applied to establish individual models of the grafting robot in line with Jack ergonomic analysis. Six human models (95th percentile, 50th percentile, and 5th percentile by height for both males and females) were employed to simulate the operating conditions and working postures in a real operating environment. The lower back and upper limb stresses of the operators were analyzed using the lower back analysis (LBA) and rapid upper limb assessment (RULA) functions in Jack. The experimental results showed that if a leg space is introduced under the robot, the operator can sit closer to the robot, which reduces the operator's level of lower back and upper limbs stress. The proper environmental layout for Taiwanese operators for minimum levels of lower back and upper limb stress are to set the grafting operation at 23.2 cm away from the operator at a height of 85 cm and with 45 cm between the rootstock and scion units.

  19. Updating Human Factors Engineering Guidelines for Conducting Safety Reviews of Nuclear Power Plants

    Energy Technology Data Exchange (ETDEWEB)

    O, J.M.; Higgins, J.; Stephen Fleger - NRC

    2011-09-19

    The U.S. Nuclear Regulatory Commission (NRC) reviews the human factors engineering (HFE) programs of applicants for nuclear power plant construction permits, operating licenses, standard design certifications, and combined operating licenses. The purpose of these safety reviews is to help ensure that personnel performance and reliability are appropriately supported. Detailed design review procedures and guidance for the evaluations is provided in three key documents: the Standard Review Plan (NUREG-0800), the HFE Program Review Model (NUREG-0711), and the Human-System Interface Design Review Guidelines (NUREG-0700). These documents were last revised in 2007, 2004 and 2002, respectively. The NRC is committed to the periodic update and improvement of the guidance to ensure that it remains a state-of-the-art design evaluation tool. To this end, the NRC is updating its guidance to stay current with recent research on human performance, advances in HFE methods and tools, and new technology being employed in plant and control room design. This paper describes the role of HFE guidelines in the safety review process and the content of the key HFE guidelines used. Then we will present the methodology used to develop HFE guidance and update these documents, and describe the current status of the update program.

  20. Osteogenic Differentiation Capacity of In Vitro Cultured Human Skeletal Muscle for Expedited Bone Tissue Engineering

    Science.gov (United States)

    Miao, Chunlei; Zhou, Lulu; Tian, Lufeng; Zhang, Yingjie; Zhang, Wei; Yang, Fanghong; Liu, Tianyi

    2017-01-01

    Expedited bone tissue engineering employs the biological stimuli to harness the intrinsic regenerative potential of skeletal muscle to trigger the reparative process in situ to improve or replace biological functions. When genetically modified with adenovirus mediated BMP2 gene transfer, muscle biopsies from animals have demonstrated success in regenerating bone within rat bony defects. However, it is uncertain whether the human adult skeletal muscle displays an osteogenic potential in vitro when a suitable biological trigger is applied. In present study, human skeletal muscle cultured in a standard osteogenic medium supplemented with dexamethasone demonstrated significant increase in alkaline phosphatase activity approximately 24-fold over control at 2-week time point. More interestingly, measurement of mRNA levels revealed the dramatic results for osteoblast transcripts of alkaline phosphatase, bone sialoproteins, transcription factor CBFA1, collagen type I, and osteocalcin. Calcified mineral deposits were demonstrated on superficial layers of muscle discs after an extended 8-week osteogenic induction. Taken together, these are the first data supporting human skeletal muscle tissue as a promising potential target for expedited bone regeneration, which of the technologies is a valuable method for tissue repair, being not only effective but also inexpensive and clinically expeditious. PMID:28210626

  1. Looking Ahead to Engineering Epimorphic Regeneration of a Human Digit or Limb.

    Science.gov (United States)

    Quijano, Lina M; Lynch, Kristen M; Allan, Christopher H; Badylak, Stephen F; Ahsan, Tabassum

    2016-06-01

    Approximately 2 million people have had limb amputations in the United States due to disease or injury, with more than 185,000 new amputations every year. The ability to promote epimorphic regeneration, or the regrowth of a biologically based digit or limb, would radically change the prognosis for amputees. This ambitious goal includes the regrowth of a large number of tissues that need to be properly assembled and patterned to create a fully functional structure. We have yet to even identify, let alone address, all the obstacles along the extended progression that limit epimorphic regeneration in humans. This review aims to present introductory fundamentals in epimorphic regeneration to facilitate design and conduct of research from a tissue engineering and regenerative medicine perspective. We describe the clinical scenario of human digit healing, featuring published reports of regenerative potential. We then broadly delineate the processes of epimorphic regeneration in nonmammalian systems and describe a few mammalian regeneration models. We give particular focus to the murine digit tip, which allows for comparative studies of regeneration-competent and regeneration-incompetent outcomes in the same animal. Finally, we describe a few forward-thinking opportunities for promoting epimorphic regeneration in humans.

  2. Analysis of OPLA scaffolds for bone engineering constructs using human jaw periosteal cells.

    Science.gov (United States)

    Alexander, Dorothea; Hoffmann, Jürgen; Munz, Adelheid; Friedrich, Björn; Geis-Gerstorfer, Jürgen; Reinert, Siegmar

    2008-03-01

    For bone regeneration constructs using human jaw periosteal cells (JPC) the extent of osteoinductive ability of different three-dimensional scaffolds is not yet established. We analyzed open-cell polylactic acid (OPLA) scaffolds for their suitability as bone engineering constructs using human JPC. Cell adhesion and spreading was visualized on the surface of scaffolds by scanning electron microscopy. JPC proliferation within OPLA scaffolds was compared with proliferation within collagen and calcium phosphate scaffolds. We found a significant increase of proliferation rates in OPLA scaffolds versus Coll/CaP scaffolds at three time points. Live-measurements of oxygen consumption within the cell-seeded scaffolds indicate that the in vitro culturing time should not exceed 12-15 days. OPLA scaffolds, which were turned out to be the most beneficial for JPC growth, were chosen for osteogenic differentiation experiments with or without BMP-2. Gene expression analyses demonstrated induction of several osteogenic genes (alkaline phosphatase, osterix, Runx-2 and insulin-like growth factor) within the 3D-scaffolds after 12 days of in vitro culturing. Element analysis by EDX spectrometry of arising nodules during osteogenesis demonstrated that JPC growing within OPLA scaffolds are able to form CaP particles. We conclude that OPLA scaffolds provide a promising environment for bone substitutes using human JPC.

  3. Engineering of a Novel Simplified Human Insulin-Like Peptide 5 Agonist.

    Science.gov (United States)

    Patil, Nitin A; Hughes, Richard A; Rosengren, K Johan; Kocan, Martina; Ang, Sheng Yu; Tailhades, Julien; Separovic, Frances; Summers, Roger J; Grosse, Johannes; Wade, John D; Bathgate, Ross A D; Hossain, Mohammed Akhter

    2016-03-10

    Insulin-like peptide 5 (INSL5) has recently been discovered as only the second orexigenic gut hormone after ghrelin. As we have previously reported, INSL5 is extremely difficult to assemble and oxidize into its two-chain three-disulfide structure. The focus of this study was to generate structure-activity relationships (SARs) of INSL5 and use it to develop a potent and simpler INSL5 mimetic with RXFP4 agonist activity. A series of human and mouse INSL5 (hINSL5/mINSL5) analogues were designed and chemically synthesized, resulting in a chimeric INSL5 analogue exhibiting more than 10-fold higher potency (0.35 nM) at human RXFP4 compared with native hINSL5 (4.57 nM). The SAR study also identified a key residue (K(A15)) in the A-chain of mINSL5 that contributes to improved RXFP4 affinity and potency of mINSL5 compared with hINSL5. This knowledge ultimately led us to engineer a minimized hINSL5 mimetic agonist that retains native hINSL5-like RXFP4 affinity and potency at human RXFP4. This minimized analogue was synthesized in 17.5-fold higher yield and in less time compared with hINSL5.

  4. Characterizing human pluripotent-stem-cell-derived vascular cells for tissue engineering applications.

    Science.gov (United States)

    Kusuma, Sravanti; Facklam, Amanda; Gerecht, Sharon

    2015-02-15

    Tissue-engineered constructs are rendered useless without a functional vasculature owing to a lack of nutrients and oxygen. Cell-based approaches to reconstruct blood vessels can yield structures that mimic native vasculature and aid transplantation. Vascular derivatives of human induced pluripotent stem cells (hiPSCs) offer opportunities to generate patient-specific therapies and potentially provide unlimited amounts of vascular cells. To be used in engineered vascular constructs and confer therapeutic benefit, vascular derivatives must exhibit additional key properties, including extracellular matrix (ECM) production to confer structural integrity and growth factor production to facilitate integration. In this study, we examine the hypothesis that vascular cells derived from hiPSCs exhibit these critical properties to facilitate their use in engineered tissues. hiPSCs were codifferentiated toward early vascular cells (EVCs), a bicellular population of endothelial cells (ECs) and pericytes, under varying low-oxygen differentiation conditions; subsequently, ECs were isolated and passaged. We found that EVCs differentiated under low-oxygen conditions produced copious amounts of collagen IV and fibronectin as well as vascular endothelial growth factor and angiopoietin 2. EVCs differentiated under atmospheric conditions did not demonstrate such abundant ECM expression, but exhibited greater expression of angiopoietin 1. Isolated ECs could proliferate up to three passages while maintaining the EC marker vascular endothelial cadherin. Isolated ECs demonstrated an increased propensity to produce ECM compared with their EVC correlates and took on an arterial-like fate. These findings illustrate that hiPSC vascular derivates hold great potential for therapeutic use and should continue to be a preferred cell source for vascular construction.

  5. A low percentage of autologous serum can replace bovine serum to engineer human nasal cartilage

    Directory of Open Access Journals (Sweden)

    F Wolf

    2008-02-01

    Full Text Available For the generation of cell-based therapeutic products, it would be preferable to avoid the use of animal-derived components. Our study thus aimed at investigating the possibility to replace foetal bovine serum (FBS with autologous serum (AS for the engineering of cartilage grafts using expanded human nasal chondrocytes (HNC. HNC isolated from 7 donors were expanded in medium containing 10% FBS or AS at different concentrations (2%, 5% and 10% and cultured in pellets using serum-free medium or in Hyaff®-11 meshes using medium containing FBS or AS. Tissue forming capacity was assessed histologically (Safranin O, immunohistochemically (type II collagen and biochemically (glycosaminoglycans -GAG- and DNA. Differences among experimental groups were assessed by Mann Whitney tests. HNC expanded under the different serum conditions proliferated at comparable rates and generated cartilaginous pellets with similar histological appearance and amounts of GAG. Tissues generated by HNC from different donors cultured in Hyaff®-11 had variable quality, but the accumulated GAG amounts were comparable among the different serum conditions. Staining intensity for collagen type II was consistent with GAG deposition. Among the different serum conditions tested, the use of 2% AS resulted in the lowest variability in the GAG contents of generated tissues. In conclusion, a low percentage of AS can replace FBS both during the expansion and differentiation of HNC and reduce the variability in the quality of the resulting engineered cartilage tissues.

  6. In vitro comparison of human fibroblasts from intact and ruptured ACL for use in tissue engineering

    Directory of Open Access Journals (Sweden)

    T Brune

    2007-12-01

    Full Text Available The present study compares fibroblasts extracted from intact and ruptured human anterior cruciate ligaments (ACL for creation of a tissue engineered ACL-construct, made of porcine small intestinal submucosal extracellular matrix (SIS-ECM seeded with these ACL cells. The comparison is based on histological, immunohistochemical and RT-PCR analyses. Differences were observed between cells in a ruptured ACL (rACL and cells in an intact ACL (iACL, particularly with regard to the expression of integrin subunits and smooth muscle actin (SMA. Despite these differences in the cell source, both cell populations behaved similarly when seeded on an SIS-ECM scaffold, with similar cell morphology, connective tissue organization and composition, SMA and integrin expression. This study shows the usefulness of naturally occurring scaffolds such as SIS-ECM for the study of cell behaviour in vitro, and illustrates the possibility to use autologous cells extracted from ruptured ACL biopsies as a source for tissue engineered ACL constructs.

  7. Engineering the human pluripotent stem cell microenvironment to direct cell fate

    Science.gov (United States)

    Hazeltine, Laurie B.; Selekman, Joshua A.; Palecek, Sean P.

    2013-01-01

    Human pluripotent stem cells (hPSCs), including both embryonic stem cells and induced pluripotent stem cells, offer a potential cell source for research, drug screening, and regenerative medicine applications due to their unique ability to self-renew or differentiate to any somatic cell type. Before the full potential of hPSCs can be realized, robust protocols must be developed to direct their fate. Cell fate decisions are based on components of the surrounding microenvironment, including soluble factors, substrate or extracellular matrix, cell-cell interactions, mechanical forces, and 2D or 3D architecture. Depending on their spatio-temporal context, these components can signal hPSCs to either self-renew or differentiate to cell types of the ectoderm, mesoderm, or endoderm. Researchers working at the interface of engineering and biology have identified various factors which can affect hPSC fate, often based on lessons from embryonic development, and they have utilized this information to design in vitro niches which can reproducibly direct hPSC fate. This review highlights culture systems that have been engineered to promote self-renewal or differentiation of hPSCs, with a focus on studies that have elucidated the contributions of specific microenvironmental cues in the context of those culture systems. We propose the use of microsystems technologies for high-throughput screening of spatial-temporal presentation of cues, as this has been demonstrated to be a powerful approach for differentiating hPSCs to desired cell types. PMID:23510904

  8. Comparative study on seeding methods of human bone marrow stromal cells in bone tissue engineering

    Institute of Scientific and Technical Information of China (English)

    齐欣; 刘建国; 常颖; 徐莘香

    2004-01-01

    Background In general the traditional static seeding method has its limitation while the dynamic seeding method reveals its advantages over traditional static method. We compared static and dynamic seeding method for human bone marrow stromal cells (hBMSCs) in bone tissue engineering.Methods DNA assay was used for detecting the maximal initial seeding concentration for static seeding. Dynamic and static seeding methods were compared, when scaffolds were loaded with hBMSCs at this maximal initial cell seeding concentration. Histology and scanning electron microscope (SEM) were examined to evaluate the distribution of cells inside the constructs. Markers encoding osteogenic genes were measured by fluorescent RT-PCR. The protocol for dynamic seeding of hBMSCs was also investigated.Results DNA assay showed that the static maximal initial seeding concentration was lower than that in dynamic seeding. Histology and SEM showed even distribution and spread of cells in the dynamically seeded constructs, while their statically seeded counterparts showed cell aggregation.Fluorescent RT-PCR again showed stronger osteogenic potential of dynamically seeded constructs.Conclusion dynamic seeding of hBMSCs is a promising technique in bone tissue engineering.

  9. Mechanical Stimulation Protocols of Human Derived Cells in Articular Cartilage Tissue Engineering - A Systematic Review.

    Science.gov (United States)

    Khozoee, Baktash; Mafi, Pouya; Mafi, Reza; Khan, Wasim S

    2017-01-01

    Mechanical stimulation is a key factor in articular cartilage generation and maintenance. Bioreactor systems have been designed and built in order to deliver specific types of mechanical stimulation. The focus has been twofold, applying a type of preconditioning in order to stimulate cell differentiation, and to simulate in vivo conditions in order to gain further insight into how cells respond to different stimulatory patterns. Due to the complex forces at work within joints, it is difficult to simulate mechanical conditions using a bioreactor. The aim of this review is to gain a deeper understanding of the complexities of mechanical stimulation protocols by comparing those employed in bioreactors in the context of tissue engineering for articular cartilage, and to consider their effects on cultured cells. Allied and Complementary Medicine 1985 to 2016, Ovid MEDLINE[R] 1946 to 2016, and Embase 1974 to 2016 were searched using key terms. Results were subject to inclusion and exclusion criteria, key findings summarised into a table and subsequently discussed. Based on this review it is overwhelmingly clear that mechanical stimulation leads to increased chondrogenic properties in the context of bioreactor articular cartilage tissue engineering using human cells. However, given the variability and lack of controlled factors between research articles, results are difficult to compare, and a standardised method of evaluating stimulation protocols proved challenging. With improved standardisation in mechanical stimulation protocol reporting, bioreactor design and building processes, along with a better understanding of joint behaviours, we hope to perform a meta-analysis on stimulation protocols and methods.

  10. Engineering Human Microbiota: Influencing Cellular and Community Dynamics for Therapeutic Applications.

    Science.gov (United States)

    Woloszynek, S; Pastor, S; Mell, J C; Nandi, N; Sokhansanj, B; Rosen, G L

    2016-01-01

    The complex relationship between microbiota, human physiology, and environmental perturbations has become a major research focus, particularly with the arrival of culture-free and high-throughput approaches for studying the microbiome. Early enthusiasm has come from results that are largely correlative, but the correlative phase of microbiome research has assisted in defining the key questions of how these microbiota interact with their host. An emerging repertoire for engineering the microbiome places current research on a more experimentally grounded footing. We present a detailed look at the interplay between microbiota and host and how these interactions can be exploited. A particular emphasis is placed on unstable microbial communities, or dysbiosis, and strategies to reestablish stability in these microbial ecosystems. These include manipulation of intermicrobial communication, development of designer probiotics, fecal microbiota transplantation, and synthetic biology.

  11. Artificial Tongue-Placed Tactile Biofeedback for perceptual supplementation: application to human disability and biomedical engineering

    CERN Document Server

    Vuillerme, Nicolas; Moreau-Gaudry, Alexandre; Demongeot, Jacques; Payan, Yohan

    2007-01-01

    The present paper aims at introducing the innovative technologies, based on the concept of "sensory substitution" or "perceptual supplementation", we are developing in the fields of human disability and biomedical engineering. Precisely, our goal is to design, develop and validate practical assistive biomedical and/technical devices and/or rehabilitating procedures for persons with disabilities, using artificial tongue-placed tactile biofeedback systems. Proposed applications are dealing with: (1) pressure sores prevention in case of spinal cord injuries (persons with paraplegia, or tetraplegia); (2) ankle proprioceptive acuity improvement for driving assistance in older and/or disabled adults; and (3) balance control improvement to prevent fall in older and/or disabled adults. This paper presents results of three feasibility studies performed on young healthy adults.

  12. Human engineering analysis for the high speed civil transport flight deck

    Science.gov (United States)

    Regal, David M.; Alter, Keith W.

    1993-01-01

    The Boeing Company is investigating the feasibility of building a second generation supersonic transport. If current studies support its viability, this airplane, known as the High Speed Civil Transport (HSCT), could be launched early in the next century. The HSCT will cruise at Mach 2.4, be over 300 feet long, have an initial range of between 5000 and 6000 NM, and carry approximately 300 passengers. We are presently involved in developing an advanced flight deck for the HSCT. As part of this effort we are undertaking a human engineering analysis that involves a top-down, mission driven approach that will allow a systematic determination of flight deck functional and information requirements. The present paper describes this work.

  13. Pluripotency of Stem Cells from Human Exfoliated Deciduous Teeth for Tissue Engineering

    Directory of Open Access Journals (Sweden)

    Vinicius Rosa

    2016-01-01

    Full Text Available Stem cells from human exfoliated deciduous teeth (SHED are highly proliferative pluripotent cells that can be retrieved from primary teeth. Although SHED are isolated from the dental pulp, their differentiation potential is not limited to odontoblasts only. In fact, SHED can differentiate into several cell types including neurons, osteoblasts, adipocytes, and endothelial cells. The high plasticity makes SHED an interesting stem cell model for research in several biomedical areas. This review will discuss key findings about the characterization and differentiation of SHED into odontoblasts, neurons, and hormone secreting cells (e.g., hepatocytes and islet-like cell aggregates. The outcomes of the studies presented here support the multipotency of SHED and their potential to be used for tissue engineering-based therapies.

  14. Engineering human cells for in vivo secretion of antibody and non-antibody therapeutic proteins.

    Science.gov (United States)

    Sánchez-Martín, David; Sanz, Laura; Álvarez-Vallina, Luis

    2011-12-01

    Purified proteins such as antibodies are widely used as therapeutic agents in clinical medicine. However, clinical-grade proteins for therapeutic use require sophisticated technologies and are extremely expensive to produce. In vivo secretion of therapeutic proteins by genetically engineered human cells may advantageously replace injection of highly purified proteins. The use of gene transfer methods circumvents problems related to large-scale production and purification and offers additional benefits by achieving sustained concentrations of therapeutic protein with a syngenic glycosylation pattern that make the protein potentially less immunogenic. The feasibility of the in vivo production of therapeutic proteins by diverse cells/tissues has now been demonstrated using different techniques, such as ex vivo genetically modified cells and in vivo gene transfer mediated by viral vectors.

  15. Characterization of the human plasma phosphoproteome using linear ion trap mass spectrometry and multiple search engines.

    Science.gov (United States)

    Carrascal, Montserrat; Gay, Marina; Ovelleiro, David; Casas, Vanessa; Gelpí, Emilio; Abian, Joaquin

    2010-02-05

    Major plasma protein families play different roles in blood physiology and hemostasis and in immunodefense. Other proteins in plasma can be involved in signaling as chemical messengers or constitute biological markers of the status of distant tissues. In this respect, the plasma phosphoproteome holds potentially relevant information on the mechanisms modulating these processes through the regulation of protein activity. In this work we describe for the first time a collection of phosphopeptides identified in human plasma using immunoaffinity separation of the seven major serum protein families from other plasma proteins, SCX fractionation, and TiO(2) purification prior to LC-MS/MS analysis. One-hundred and twenty-seven phosphosites in 138 phosphopeptides mapping 70 phosphoproteins were identified with FDR < 1%. A high-confidence collection of phosphosites was obtained using a combined search with the OMSSA, SEQUEST, and Phenyx search engines.

  16. Materials Development Program: Ceramic Technology Project bibliography, 1984--1992

    Energy Technology Data Exchange (ETDEWEB)

    1994-03-01

    The Ceramic Technology [for Advanced Heat Engines] Project was begun in 1983 to meet the ceramic materials needs of the companion DOE automotive engine program, the Advanced Gas Turbine (AGT) project, and the Heavy Duty Transport (low-heat-rejection, heavy-duty diesel) project. Goal is to develop an industry technology base for reliable and cost effective ceramics for applications in advanced automotive gas turbine and diesel engines. Research areas were identified following extensive input from industry and academia. Majority of research is done by industry (60%); work is also done at colleges and universities, in-house, and at other national laboratories and government agencies. In the beginning, reliability of ceramic components was the key issue. The reliability issues have largely been met and, at the present time, cost is the driving issue, especially in light of the highly cost-sensitive automotive market. Emphasis of the program has now been shifted toward developing cost-effective ceramic components for high-performance engines in the near-term. This bibliography is a compilation of publications done in conjunction with the Ceramic Technology Project since its beginning. Citations were obtained from reports done by participants in the project. We have tried to limit citations to those published and easily located. The end date of 1992 was selected.

  17. Cohesiveness and Interpersonal Attraction: A Selected, Annotated Basic Bibliography.

    Science.gov (United States)

    Beebe, Steven A.; Webb, Marsha Short

    This 43-item selected, annotated bibliography on cohesiveness and interpersonal attraction includes books, journal articles and conference papers published between 1953 and 1990. The bibliography is in two parts. The first part consists of key sources, most of which have extensive bibliographies or lists of references. The second part consists of…

  18. Knowledge-based personalized search engine for the Web-based Human Musculoskeletal System Resources (HMSR) in biomechanics.

    Science.gov (United States)

    Dao, Tien Tuan; Hoang, Tuan Nha; Ta, Xuan Hien; Tho, Marie Christine Ho Ba

    2013-02-01

    Human musculoskeletal system resources of the human body are valuable for the learning and medical purposes. Internet-based information from conventional search engines such as Google or Yahoo cannot response to the need of useful, accurate, reliable and good-quality human musculoskeletal resources related to medical processes, pathological knowledge and practical expertise. In this present work, an advanced knowledge-based personalized search engine was developed. Our search engine was based on a client-server multi-layer multi-agent architecture and the principle of semantic web services to acquire dynamically accurate and reliable HMSR information by a semantic processing and visualization approach. A security-enhanced mechanism was applied to protect the medical information. A multi-agent crawler was implemented to develop a content-based database of HMSR information. A new semantic-based PageRank score with related mathematical formulas were also defined and implemented. As the results, semantic web service descriptions were presented in OWL, WSDL and OWL-S formats. Operational scenarios with related web-based interfaces for personal computers and mobile devices were presented and analyzed. Functional comparison between our knowledge-based search engine, a conventional search engine and a semantic search engine showed the originality and the robustness of our knowledge-based personalized search engine. In fact, our knowledge-based personalized search engine allows different users such as orthopedic patient and experts or healthcare system managers or medical students to access remotely into useful, accurate, reliable and good-quality HMSR information for their learning and medical purposes.

  19. Generation of functionally competent and durable engineered blood vessels from human induced pluripotent stem cells.

    Science.gov (United States)

    Samuel, Rekha; Daheron, Laurence; Liao, Shan; Vardam, Trupti; Kamoun, Walid S; Batista, Ana; Buecker, Christa; Schäfer, Richard; Han, Xiaoxing; Au, Patrick; Scadden, David T; Duda, Dan G; Fukumura, Dai; Jain, Rakesh K

    2013-07-30

    Efficient generation of competent vasculogenic cells is a critical challenge of human induced pluripotent stem (hiPS) cell-based regenerative medicine. Biologically relevant systems to assess functionality of the engineered vessels in vivo are equally important for such development. Here, we report a unique approach for the derivation of endothelial precursor cells from hiPS cells using a triple combination of selection markers--CD34, neuropilin 1, and human kinase insert domain-containing receptor--and an efficient 2D culture system for hiPS cell-derived endothelial precursor cell expansion. With these methods, we successfully generated endothelial cells (ECs) from hiPS cells obtained from healthy donors and formed stable functional blood vessels in vivo, lasting for 280 d in mice. In addition, we developed an approach to generate mesenchymal precursor cells (MPCs) from hiPS cells in parallel. Moreover, we successfully generated functional blood vessels in vivo using these ECs and MPCs derived from the same hiPS cell line. These data provide proof of the principle that autologous hiPS cell-derived vascular precursors can be used for in vivo applications, once safety and immunological issues of hiPS-based cellular therapy have been resolved. Additionally, the durability of hiPS-derived blood vessels in vivo demonstrates a potential translation of this approach in long-term vascularization for tissue engineering and treatment of vascular diseases. Of note, we have also successfully generated ECs and MPCs from type 1 diabetic patient-derived hiPS cell lines and use them to generate blood vessels in vivo, which is an important milestone toward clinical translation of this approach.

  20. Bibliography on Biomass Feedstock Research: 1978-2002

    Energy Technology Data Exchange (ETDEWEB)

    Cushman, J.H.

    2003-05-01

    This report provides bibliographic citations for more than 1400 reports on biomass feedstock development published by Oak Ridge National Laboratory and its collaborators from 1978 through 2002. Oak Ridge National Laboratory is engaged in analysis of biomass resource supplies, research on the sustainability of feedstock resources, and research on feedstock engineering and infrastructure. From 1978 until 2002, Oak Ridge National Laboratory also provided technical leadership for the U.S. Department of Energy's Bioenergy Feedstock Development Program (BFDP), which supported research to identify and develop promising energy crops. This bibliography lists reports published by Oak Ridge National Laboratory and by its collaborators in the BFDP, including graduate student theses and dissertations.