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Sample records for human cocktail party

  1. Edge-clique graphs of cocktail parties have unbounded rankwidth

    CERN Document Server

    Chang, Maw-Shang; Liu, Ching-Hao

    2012-01-01

    In an attempt to find a polynomial-time algorithm for the edge-clique cover problem on cographs we tried to prove that the edge-clique graphs of cographs have bounded rankwidth. However, this is not the case. In this note we show that the edge-clique graphs of cocktail party graphs have unbounded rankwidth.

  2. From Contests to Cocktail Parties: Strategies for Impromptu Speaking.

    Science.gov (United States)

    Katchen, Johanna E.

    Classroom techniques are offered for teaching students how to present a spontaneous (impromptu) speech in a second language, such as for use at a job interview or cocktail party. It is first suggested that such classroom activity be graded minimally to help lower students' level of apprehension. Then strategies for approaching impromptu speech…

  3. How does spatial hearing affect cocktail party conversations?

    Science.gov (United States)

    Shinn-Cunningham, Barbara G.

    2003-04-01

    Although spatial cues are not a dominant factor in auditory scene analysis by humans, spatial separation of a target talker and an interfering sound source often increases the intelligibility of the target. A large portion of this improvement arises from a simple acoustic effect: separating the target and interferer generally increases the target-to-interferer energy ratio at one ear. When the target is near threshold, binaural processing provides an additional small, but important improvement. Finally, in conditions where both target and interferer are audible but are difficult to segregate, spatial separation can improve the ability to stream the sources and interpret the target message. The echoes and reverberation present in everyday environments influence all of these factors, altering the target-to-interferer energy ratio, the effectiveness of binaural processing, and the ability to stream the sources. A number of studies will be reviewed to demonstrate how these different factors influence speech intelligibility and affect cocktail party conversations in everyday environments. [Work supported by the Air Force Office of Scientific Research and the Alfred P. Sloan Foundation.

  4. Musical training, individual differences and the cocktail party problem.

    Science.gov (United States)

    Swaminathan, Jayaganesh; Mason, Christine R; Streeter, Timothy M; Best, Virginia; Kidd, Gerald; Patel, Aniruddh D

    2015-06-26

    Are musicians better able to understand speech in noise than non-musicians? Recent findings have produced contradictory results. Here we addressed this question by asking musicians and non-musicians to understand target sentences masked by other sentences presented from different spatial locations, the classical 'cocktail party problem' in speech science. We found that musicians obtained a substantial benefit in this situation, with thresholds ~6 dB better than non-musicians. Large individual differences in performance were noted particularly for the non-musically trained group. Furthermore, in different conditions we manipulated the spatial location and intelligibility of the masking sentences, thus changing the amount of 'informational masking' (IM) while keeping the amount of 'energetic masking' (EM) relatively constant. When the maskers were unintelligible and spatially separated from the target (low in IM), musicians and non-musicians performed comparably. These results suggest that the characteristics of speech maskers and the amount of IM can influence the magnitude of the differences found between musicians and non-musicians in multiple-talker "cocktail party" environments. Furthermore, considering the task in terms of the EM-IM distinction provides a conceptual framework for future behavioral and neuroscientific studies which explore the underlying sensory and cognitive mechanisms contributing to enhanced "speech-in-noise" perception by musicians.

  5. Complexity of Cocktail Party Graph and Crown Graph

    Directory of Open Access Journals (Sweden)

    S. N. Daoud

    2012-01-01

    Full Text Available Problem statement: The number of spanning trees τ(G in graphs (networks was an important invariant. Approach: Using the properties of the Chebyshev polynomials of the second kind and the linear algebra techniques to evaluate the associated determinants. Results: The complexity, number of spanning trees, of the cocktail party graph on 2n vertices, given in detail in the text was proved. Also the complexity of the crown graph on 2n vertices was shown to had the value nn-2 (n-1 (n-2n-1. Conclusion: The number of spanning trees τ(G in graphs (networks is an important invariant. The evaluation of this number and analyzing its behavior is not only interesting from a mathematical (computational perspective, but also, it is an important measure of reliability of a network and designing electrical circuits. Some computationally hard problems such as the travelling salesman problem can be solved approximately by using spanning trees. Due to the high dependence of the network design and reliability on the graph theory we introduced the above important theorems and lemmas and their proofs.

  6. Visual input enhances selective speech envelope tracking in auditory cortex at a "cocktail party".

    Science.gov (United States)

    Zion Golumbic, Elana; Cogan, Gregory B; Schroeder, Charles E; Poeppel, David

    2013-01-23

    Our ability to selectively attend to one auditory signal amid competing input streams, epitomized by the "Cocktail Party" problem, continues to stimulate research from various approaches. How this demanding perceptual feat is achieved from a neural systems perspective remains unclear and controversial. It is well established that neural responses to attended stimuli are enhanced compared with responses to ignored ones, but responses to ignored stimuli are nonetheless highly significant, leading to interference in performance. We investigated whether congruent visual input of an attended speaker enhances cortical selectivity in auditory cortex, leading to diminished representation of ignored stimuli. We recorded magnetoencephalographic signals from human participants as they attended to segments of natural continuous speech. Using two complementary methods of quantifying the neural response to speech, we found that viewing a speaker's face enhances the capacity of auditory cortex to track the temporal speech envelope of that speaker. This mechanism was most effective in a Cocktail Party setting, promoting preferential tracking of the attended speaker, whereas without visual input no significant attentional modulation was observed. These neurophysiological results underscore the importance of visual input in resolving perceptual ambiguity in a noisy environment. Since visual cues in speech precede the associated auditory signals, they likely serve a predictive role in facilitating auditory processing of speech, perhaps by directing attentional resources to appropriate points in time when to-be-attended acoustic input is expected to arrive.

  7. Web party effect: a cocktail party effect in the web environment.

    Science.gov (United States)

    Rigutti, Sara; Fantoni, Carlo; Gerbino, Walter

    2015-01-01

    In goal-directed web navigation, labels compete for selection: this process often involves knowledge integration and requires selective attention to manage the dizziness of web layouts. Here we ask whether the competition for selection depends on all web navigation options or only on those options that are more likely to be useful for information seeking, and provide evidence in favor of the latter alternative. Participants in our experiment navigated a representative set of real websites of variable complexity, in order to reach an information goal located two clicks away from the starting home page. The time needed to reach the goal was accounted for by a novel measure of home page complexity based on a part of (not all) web options: the number of links embedded within web navigation elements weighted by the number and type of embedding elements. Our measure fully mediated the effect of several standard complexity metrics (the overall number of links, words, images, graphical regions, the JPEG file size of home page screenshots) on information seeking time and usability ratings. Furthermore, it predicted the cognitive demand of web navigation, as revealed by the duration judgment ratio (i.e., the ratio of subjective to objective duration of information search). Results demonstrate that focusing on relevant links while ignoring other web objects optimizes the deployment of attentional resources necessary to navigation. This is in line with a web party effect (i.e., a cocktail party effect in the web environment): users tune into web elements that are relevant for the achievement of their navigation goals and tune out all others.

  8. Experimental Study of Generalized Subspace Filters for the Cocktail Party Situation

    DEFF Research Database (Denmark)

    Christensen, Knud Bank; Christensen, Mads Græsbøll; Boldt, Jesper B.

    2016-01-01

    This paper investigates the potential performance of generalized subspace filters for speech enhancement in cocktail party situations with very poor signal/noise ratio, e.g. down to -15 dB. Performance metrics output signal/noise ratio, signal/ distortion ratio, speech quality rating and speech...

  9. Selective Attention Enhances Beta-Band Cortical Oscillation to Speech under "Cocktail-Party" Listening Conditions.

    Science.gov (United States)

    Gao, Yayue; Wang, Qian; Ding, Yu; Wang, Changming; Li, Haifeng; Wu, Xihong; Qu, Tianshu; Li, Liang

    2017-01-01

    Human listeners are able to selectively attend to target speech in a noisy environment with multiple-people talking. Using recordings of scalp electroencephalogram (EEG), this study investigated how selective attention facilitates the cortical representation of target speech under a simulated "cocktail-party" listening condition with speech-on-speech masking. The result shows that the cortical representation of target-speech signals under the multiple-people talking condition was specifically improved by selective attention relative to the non-selective-attention listening condition, and the beta-band activity was most strongly modulated by selective attention. Moreover, measured with the Granger Causality value, selective attention to the single target speech in the mixed-speech complex enhanced the following four causal connectivities for the beta-band oscillation: the ones (1) from site FT7 to the right motor area, (2) from the left frontal area to the right motor area, (3) from the central frontal area to the right motor area, and (4) from the central frontal area to the right frontal area. However, the selective-attention-induced change in beta-band causal connectivity from the central frontal area to the right motor area, but not other beta-band causal connectivities, was significantly correlated with the selective-attention-induced change in the cortical beta-band representation of target speech. These findings suggest that under the "cocktail-party" listening condition, the beta-band oscillation in EEGs to target speech is specifically facilitated by selective attention to the target speech that is embedded in the mixed-speech complex. The selective attention-induced unmasking of target speech may be associated with the improved beta-band functional connectivity from the central frontal area to the right motor area, suggesting a top-down attentional modulation of the speech-motor process.

  10. Executive Function, Visual Attention and the Cocktail Party Problem in Musicians and Non-Musicians

    Science.gov (United States)

    Clayton, Kameron K.; Swaminathan, Jayaganesh; Yazdanbakhsh, Arash; Zuk, Jennifer; Patel, Aniruddh D.; Kidd, Gerald

    2016-01-01

    The goal of this study was to investigate how cognitive factors influence performance in a multi-talker, “cocktail-party” like environment in musicians and non-musicians. This was achieved by relating performance in a spatial hearing task to cognitive processing abilities assessed using measures of executive function (EF) and visual attention in musicians and non-musicians. For the spatial hearing task, a speech target was presented simultaneously with two intelligible speech maskers that were either colocated with the target (0° azimuth) or were symmetrically separated from the target in azimuth (at ±15°). EF assessment included measures of cognitive flexibility, inhibition control and auditory working memory. Selective attention was assessed in the visual domain using a multiple object tracking task (MOT). For the MOT task, the observers were required to track target dots (n = 1,2,3,4,5) in the presence of interfering distractor dots. Musicians performed significantly better than non-musicians in the spatial hearing task. For the EF measures, musicians showed better performance on measures of auditory working memory compared to non-musicians. Furthermore, across all individuals, a significant correlation was observed between performance on the spatial hearing task and measures of auditory working memory. This result suggests that individual differences in performance in a cocktail party-like environment may depend in part on cognitive factors such as auditory working memory. Performance in the MOT task did not differ between groups. However, across all individuals, a significant correlation was found between performance in the MOT and spatial hearing tasks. A stepwise multiple regression analysis revealed that musicianship and performance on the MOT task significantly predicted performance on the spatial hearing task. Overall, these findings confirm the relationship between musicianship and cognitive factors including domain-general selective attention and

  11. A cocktail party model of spatial release from masking by both noise and speech interferers.

    Science.gov (United States)

    Jones, Gary L; Litovsky, Ruth Y

    2011-09-01

    A mathematical formula for estimating spatial release from masking (SRM) in a cocktail party environment would be useful as a simpler alternative to computationally intensive algorithms and may enhance understanding of underlying mechanisms. The experiment presented herein was designed to provide a strong test of a model that divides SRM into contributions of asymmetry and angular separation [Bronkhorst (2000). Acustica 86, 117-128] and to examine whether that model can be extended to include speech maskers. Across masker types the contribution to SRM of angular separation of maskers from the target was found to grow at a diminishing rate as angular separation increased within the frontal hemifield, contrary to predictions of the model. Speech maskers differed from noise maskers in the overall magnitude of SRM and in the contribution of angular separation (both greater for speech). These results were used to develop a modified model that achieved good fits to data for noise maskers (ρ=0.93) and for speech maskers (ρ=0.94) while using the same functions to describe separation and asymmetry components of SRM for both masker types. These findings suggest that this approach can be used to accurately model SRM for speech maskers in addition to primarily "energetic" noise maskers.

  12. A cocktail party model of spatial release from masking by both noise and speech interferers a)

    Science.gov (United States)

    Jones, Gary L.; Litovsky, Ruth Y.

    2011-01-01

    A mathematical formula for estimating spatial release from masking (SRM) in a cocktail party environment would be useful as a simpler alternative to computationally intensive algorithms and may enhance understanding of underlying mechanisms. The experiment presented herein was designed to provide a strong test of a model that divides SRM into contributions of asymmetry and angular separation [Bronkhorst (2000). Acustica 86, 117–128] and to examine whether that model can be extended to include speech maskers. Across masker types the contribution to SRM of angular separation of maskers from the target was found to grow at a diminishing rate as angular separation increased within the frontal hemifield, contrary to predictions of the model. Speech maskers differed from noise maskers in the overall magnitude of SRM and in the contribution of angular separation (both greater for speech). These results were used to develop a modified model that achieved good fits to data for noise maskers (ρ = 0.93) and for speech maskers (ρ = 0.94) while using the same functions to describe separation and asymmetry components of SRM for both masker types. These findings suggest that this approach can be used to accurately model SRM for speech maskers in addition to primarily “energetic” noise maskers. PMID:21895087

  13. Sound stream segregation: a neuromorphic approach to solve the ‘cocktail party problem’ in real-time

    Directory of Open Access Journals (Sweden)

    Chetan Singh Thakur

    2015-09-01

    Full Text Available The human auditory system has the ability to segregate complex auditory scenes into a foreground component and a background, allowing us to listen to specific speech sounds from a mixture of sounds. Selective attention plays a crucial role in this process, colloquially known as the ‘cocktail party effect’. It has not been possible to build a machine that can emulate this human ability in real-time. Here, we have developed a framework for the implementation of a neuromorphic sound segregation algorithm in a Field Programmable Gate Array (FPGA. This algorithm is based on the principles of temporal coherence and uses an attention signal to separate a target sound stream from background noise. Temporal coherence implies that auditory features belonging to the same sound source are coherently modulated and evoke highly correlated neural response patterns. The basis for this form of sound segregation is that responses from pairs of channels that are strongly positively correlated belong to the same stream, while channels that are uncorrelated or anti-correlated belong to different streams. In our framework, we have used a neuromorphic cochlea as a frontend sound analyser to extract spatial information of the sound input, which then passes through band pass filters that extract the sound envelope at various modulation rates. Further stages include feature extraction and mask generation, which is finally used to reconstruct the targeted sound. Using sample tonal and speech mixtures, we show that our FPGA architecture is able to segregate sound sources in real-time. The accuracy of segregation is indicated by the high signal-to-noise ratio (SNR of the segregated stream (90, 77 and 55 dB for simple tone, complex tone and speech, respectively as compared to the SNR of the mixture waveform (0 dB. This system may be easily extended for the segregation of complex speech signals, and may thus find various applications in electronic devices such as for

  14. Solutions to the cocktail party problem in insects: selective filters, spatial release from masking and gain control in tropical crickets.

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    Arne K D Schmidt

    Full Text Available Insects often communicate by sound in mixed species choruses; like humans and many vertebrates in crowded social environments they thus have to solve cocktail-party-like problems in order to ensure successful communication with conspecifics. This is even more a problem in species-rich environments like tropical rainforests, where background noise levels of up to 60 dB SPL have been measured.Using neurophysiological methods we investigated the effect of natural background noise (masker on signal detection thresholds in two tropical cricket species Paroecanthus podagrosus and Diatrypa sp., both in the laboratory and outdoors. We identified three 'bottom-up' mechanisms which contribute to an excellent neuronal representation of conspecific signals despite the masking background. First, the sharply tuned frequency selectivity of the receiver reduces the amount of masking energy around the species-specific calling song frequency. Laboratory experiments yielded an average signal-to-noise ratio (SNR of -8 dB, when masker and signal were broadcast from the same side. Secondly, displacing the masker by 180° from the signal improved SNRs by further 6 to 9 dB, a phenomenon known as spatial release from masking. Surprisingly, experiments carried out directly in the nocturnal rainforest yielded SNRs of about -23 dB compared with those in the laboratory with the same masker, where SNRs reached only -14.5 and -16 dB in both species. Finally, a neuronal gain control mechanism enhances the contrast between the responses to signals and the masker, by inhibition of neuronal activity in interstimulus intervals.Thus, conventional speaker playbacks in the lab apparently do not properly reconstruct the masking noise situation in a spatially realistic manner, since under real world conditions multiple sound sources are spatially distributed in space. Our results also indicate that without knowledge of the receiver properties and the spatial release mechanisms the

  15. Switching of auditory attention in "cocktail-party" listening: ERP evidence of cueing effects in younger and older adults.

    Science.gov (United States)

    Getzmann, Stephan; Jasny, Julian; Falkenstein, Michael

    2017-02-01

    Verbal communication in a "cocktail-party situation" is a major challenge for the auditory system. In particular, changes in target speaker usually result in declined speech perception. Here, we investigated whether speech cues indicating a subsequent change in target speaker reduce the costs of switching in younger and older adults. We employed event-related potential (ERP) measures and a speech perception task, in which sequences of short words were simultaneously presented by four speakers. Changes in target speaker were either unpredictable or semantically cued by a word within the target stream. Cued changes resulted in a less decreased performance than uncued changes in both age groups. The ERP analysis revealed shorter latencies in the change-related N400 and late positive complex (LPC) after cued changes, suggesting an acceleration in context updating and attention switching. Thus, both younger and older listeners used semantic cues to prepare changes in speaker setting.

  16. Focused and divided attention in a simulated cocktail-party situation: ERP evidence from younger and older adults.

    Science.gov (United States)

    Getzmann, Stephan; Golob, Edward J; Wascher, Edmund

    2016-05-01

    Speech perception under complex listening conditions usually decreases in aging. This is especially true for listening conditions requiring divided attention among 2 and more relevant speakers. Using a speech perception task and event-related potential measures, we studied the ability of younger and older adults to attend to speech information from a single-target speaker (focused attention) or from 2 different (alternative) target speakers (divided attention). The focused and divided attention conditions were presented either in silence or in the presence of 3 concurrent speakers. In the presence of concurrent speakers, older participants showed worse performance with divided versus focused attention. In contrast, there was no effect of attention condition for the younger adults. Relative to the young, event-related potential analysis in older subjects indicated a decline in preparatory activity for the critical speech information (a delayed and smaller contingent negative variation), and delayed attentional control (indicated by a longer P2 latency). Standardized low-resolution brain electromagnetic tomography revealed that the age-related decline in preparatory activity was associated with reduced activation of medial and superior frontal gyrus and anterior cingulate gyrus. The results suggest that age-related differences in these prefrontal brain areas reflect declines in preparatory attention and gating of subsequent task-related speech information, especially under conditions of divided attention. These findings may reflect mechanisms relating to impaired speech perception by older people in "cocktail-party" listening situations.

  17. Using auditory pre-information to solve the cocktail-party problem: electrophysiological evidence for age-specific differences

    Directory of Open Access Journals (Sweden)

    Stephan eGetzmann

    2014-12-01

    Full Text Available Speech understanding in complex and dynamic listening environments requires (a auditory scene analysis, namely auditory object formation and segregation, and (b allocation of the attentional focus to the talker of interest. There is evidence that pre-information is actively used to facilitate these two aspects of the so-called cocktail-party problem. Here, a simulated multi-talker scenario was combined with electroencephalography to study scene analysis and allocation of attention in young and middle-aged adults. Sequences of short words (combinations of brief company names and stock-price values from four talkers at different locations were simultaneously presented, and the detection of target names and the discrimination between critical target values were assessed. Immediately prior to speech sequences, auditory pre-information was provided via cues that either prepared auditory scene analysis or attentional focusing, or non-specific pre-information was given. While performance was generally better in younger than older participants, both age groups benefited from auditory pre-information. The analysis of the cue-related event-related potentials revealed age-specific differences in the use of pre-cues: Younger adults showed a pronounced N2 component, suggesting early inhibition of concurrent speech stimuli; older adults exhibited a stronger late P3 component, suggesting increased resource allocation to process the pre-information. In sum, the results argue for an age-specific utilization of auditory pre-information to improve listening in complex dynamic auditory environments.

  18. Visually guided auditory attention in a dynamic "cocktail-party" speech perception task: ERP evidence for age-related differences.

    Science.gov (United States)

    Getzmann, Stephan; Wascher, Edmund

    2017-02-01

    Speech understanding in the presence of concurring sound is a major challenge especially for older persons. In particular, conversational turn-takings usually result in switch costs, as indicated by declined speech perception after changes in the relevant target talker. Here, we investigated whether visual cues indicating the future position of a target talker may reduce the costs of switching in younger and older adults. We employed a speech perception task, in which sequences of short words were simultaneously presented by three talkers, and analysed behavioural measures and event-related potentials (ERPs). Informative cues resulted in increased performance after a spatial change in target talker compared to uninformative cues, not indicating the future target position. Especially the older participants benefited from knowing the future target position in advance, indicated by reduced response times after informative cues. The ERP analysis revealed an overall reduced N2, and a reduced P3b to changes in the target talker location in older participants, suggesting reduced inhibitory control and context updating. On the other hand, a pronounced frontal late positive complex (f-LPC) to the informative cues indicated increased allocation of attentional resources to changes in target talker in the older group, in line with the decline-compensation hypothesis. Thus, knowing where to listen has the potential to compensate for age-related decline in attentional switching in a highly variable cocktail-party environment.

  19. Enhanced radioimmunotherapeutic efficacy of a monoclonal antibody cocktail against SMMC—7721 human hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    SONGYIQIANG; GENFENGWANG; 等

    1998-01-01

    The improved tumoricidal effect of the radioantibody mixture (“cocktail”)has been reported recently for the treatment of colon tumor.In the present study,we demonstrated the enhanced radioimmunotherapeutic efficacy of a monoclonal antibody (MAb) cocktail against human hepatocellular carcinoma.Therapeutic efficacy was determined by measuring the change in tumor size over a period,determining the percentage of growth inhibition of each treatment at various times after radioantibody therapy.Radioimmunotherapy of SMMC-7721 human hepatoma xenografts in athymic unde mice with combination of 131Ilabeled Hepama-1 and 131 I-labeled 9403 mouse MAbs was more effective than using either Hepeam-1 or 9403 MAb alone The MAb cocktail could target a greater number of hepatoma cells and increase the magnitude of hepatoma cell uptake of radioantibodies.The in vitro results explain the enhanced effect of the MAb cocktail in in vivo model system.

  20. Human mast cell mediator cocktail excites neurons in human and guinea-pig enteric nervous system.

    Science.gov (United States)

    Schemann, M; Michel, K; Ceregrzyn, M; Zeller, F; Seidl, S; Bischoff, S C

    2005-04-01

    Neuroimmune interactions are an integral part of gut physiology and involved in the pathogenesis of inflammatory and functional bowel disorders. Mast cells and their mediators are important conveyors in the communication from the innate enteric immune system to the enteric nervous system (ENS). However, it is not known whether a mediator cocktail released from activated human mast cells affects neural activity in the ENS. We used the Multi-Site Optical Recording Technique to image single cell activity in guinea-pig and human ENS after application of a mast cell mediator cocktail (MCMC) that was released from isolated human intestinal mucosa mast cells stimulated by IgE-receptor cross-linking. Local application of MCMC onto individual ganglia evoked an excitatory response consisting of action potential discharge. This excitatory response occurred in 31%, 38% or 11% neurons of guinea-pig submucous plexus, human submucous plexus, or guinea-pig myenteric plexus, respectively. Compound action potentials from nerve fibres or fast excitatory synaptic inputs were not affected by MCMC. This study demonstrates immunoneural signalling in the human gut and revealed for the first time that an MCMC released from stimulated human intestinal mast cells induces excitatory actions in the human and guinea-pig ENS.

  1. A Combination Cocktail Improves Spatial Attention in a Canine Model of Human Aging and Alzheimer's disease

    Science.gov (United States)

    Head, Elizabeth; Murphey, Heather L.; Dowling, Amy L.S.; McCarty, Katie L.; Bethel, Samuel R.; Nitz, Jonathan A.; Pleiss, Melanie; Vanrooyen, Jenna; Grossheim, Mike; Smiley, Jeffery R.; Murphy, M. Paul; Beckett, Tina L.; Pagani, Dieter; Bresch, Frederick; Hendrix, Curt

    2014-01-01

    Alzheimer's disease (AD) involves multiple pathological processes in the brain, including increased inflammation and oxidative damage, as well as the accumulation of beta-amyloid (Aβ) plaques. We hypothesized that a combinatorial therapeutic approach to target these multiple pathways may provide cognitive and neuropathological benefits for AD patients. To test this hypothesis, we used a canine model of human aging and AD. Aged dogs naturally develop learning and memory impairments, human-type Aβ deposits and oxidative damage in the brain. Thus, 9 aged beagles (98-115 months) were treated with a medical food cocktail containing (1) an extract of turmeric containing 95% curcuminoids; (2) an extract of green tea containing 50% epigallocatechingallate; (3) N-acetyl cysteine; (4) R-alpha lipoic acid; and (5) an extract of black pepper containing 95% piperine. Nine similarly aged dogs served as placebo-treated controls. After 3 months of treatment, 13 dogs completed a variable distance landmark task used as a measure of spatial attention. As compared to placebo-treated animals, dogs receiving the medical food cocktail had significantly lower error scores (t(11)=4.3, p=0.001) and were more accurate across all distances (F(1,9)=20.7, p=0.001), suggesting an overall improvement in spatial attention. Measures of visual discrimination learning, executive function and spatial memory, and levels of brain and CSF Aβ were unaffected by the cocktail. Our results indicate that this medical food cocktail may be beneficial for improving spatial attention and motivation deficits associated with impaired cognition in aging and AD. PMID:22886019

  2. Development of a mouse monoclonal antibody cocktail for post-exposure rabies prophylaxis in humans.

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    Thomas Müller

    Full Text Available As the demand for rabies post-exposure prophylaxis (PEP treatments has increased exponentially in recent years, the limited supply of human and equine rabies immunoglobulin (HRIG and ERIG has failed to provide the required passive immune component in PEP in countries where canine rabies is endemic. Replacement of HRIG and ERIG with a potentially cheaper and efficacious alternative biological for treatment of rabies in humans, therefore, remains a high priority. In this study, we set out to assess a mouse monoclonal antibody (MoMAb cocktail with the ultimate goal to develop a product at the lowest possible cost that can be used in developing countries as a replacement for RIG in PEP. Five MoMAbs, E559.9.14, 1112-1, 62-71-3, M727-5-1, and M777-16-3, were selected from available panels based on stringent criteria, such as biological activity, neutralizing potency, binding specificity, spectrum of neutralization of lyssaviruses, and history of each hybridoma. Four of these MoMAbs recognize epitopes in antigenic site II and one recognizes an epitope in antigenic site III on the rabies virus (RABV glycoprotein, as determined by nucleotide sequence analysis of the glycoprotein gene of unique MoMAb neutralization-escape mutants. The MoMAbs were produced under Good Laboratory Practice (GLP conditions. Unique combinations (cocktails were prepared, using different concentrations of the MoMAbs that were capable of targeting non-overlapping epitopes of antigenic sites II and III. Blind in vitro efficacy studies showed the MoMab cocktails neutralized a broad spectrum of lyssaviruses except for lyssaviruses belonging to phylogroups II and III. In vivo, MoMAb cocktails resulted in protection as a component of PEP that was comparable to HRIG. In conclusion, all three novel combinations of MoMAbs were shown to have equal efficacy to HRIG and therefore could be considered a potentially less expensive alternative biological agent for use in PEP and prevention of

  3. The Role of Top-Down Attention in the Cocktail Party: Revisiting Cherry’s Experiment after Sixty Years

    DEFF Research Database (Denmark)

    Marchegiani, Letizia; Karadogan, Seliz; Andersen, Tobias

    2011-01-01

    . Based on such simulations we expect that under strong top-down attention pattern recognition is improved as the model can compensate for noise and confounders. We next investigate the role of temporal and spectral overlaps and speech intelligibility in humans, and how the presence of a task influences...... their relation. For this purpose, we perform behavioral experiments inspired by Cherry’s classic experiments carried out almost sixty years ago. We make participants listen to a mono signal consisting of two different narratives pronounced by a speech synthesizer under two different conditions. In the first case...

  4. The Role of Top-down Attention in the Cocktail Party: Revisiting Cherry's Experiment after Sixty Years

    DEFF Research Database (Denmark)

    Marchegiani, Letizia; Karadogan, Seliz; Andersen, Taja

    2011-01-01

    . Based on such simulations we expect that under strong top-down attention pattern recognition is improved as the model can compensate for noise and confounders. We next investigate the role of temporal and spectral overlaps and speech intelligibility in humans, and how the presence of a task influences...... their relation. For this purpose, we perform behavioral experiments inspired by Cherry's classic experiments carried out almost sixty years ago. We make participants listen to a mono signal consisting of two different narratives pronounced by a speech synthesizer under two different conditions. In the first case...

  5. Communist Party of China and Human Rights Protection for Criminals

    Institute of Scientific and Technical Information of China (English)

    YAN JIA; PEI JUNJIE

    2011-01-01

    Protection of criminals' human rights is an important issue that has received full attention at home and abroad.The Communlst Party of China (CPC) has always attached much importance to the protection of criminals' human rights.Since the founding of New China,the Party and the state have paid full attention to protecting the human rights of criminals.

  6. Safety analysis of a Russian phage cocktail: From MetaGenomic analysis to oral application in healthy human subjects

    Energy Technology Data Exchange (ETDEWEB)

    McCallin, Shawna, E-mail: semccallin@yahoo.com [Nestlé Research Centre, Nestec Ltd, Vers-chez-les-Blanc, CH-1000 Lausanne 26 (Switzerland); Alam Sarker, Shafiqul, E-mail: sasarker@icddrb.org [International Centre for Diarrhoeal Diseases Research, Bangladesh (icddr,b), 68 Shaheed Tajuddin Ahmed Sharani, Mohakhali, Dhaka 1212 (Bangladesh); Barretto, Caroline, E-mail: Caroline.Barretto@rdls.nestle.com [Nestlé Research Centre, Nestec Ltd, Vers-chez-les-Blanc, CH-1000 Lausanne 26 (Switzerland); Sultana, Shamima, E-mail: shamima@icddrb.org [International Centre for Diarrhoeal Diseases Research, Bangladesh (icddr,b), 68 Shaheed Tajuddin Ahmed Sharani, Mohakhali, Dhaka 1212 (Bangladesh); Berger, Bernard, E-mail: bernard.berger@rdls.nestle.com [Nestlé Research Centre, Nestec Ltd, Vers-chez-les-Blanc, CH-1000 Lausanne 26 (Switzerland); Huq, Sayeda, E-mail: sayeeda@mail.icddrb.org [International Centre for Diarrhoeal Diseases Research, Bangladesh (icddr,b), 68 Shaheed Tajuddin Ahmed Sharani, Mohakhali, Dhaka 1212 (Bangladesh); Krause, Lutz, E-mail: ltz.krause@gmail.com [Nestlé Research Centre, Nestec Ltd, Vers-chez-les-Blanc, CH-1000 Lausanne 26 (Switzerland); Bibiloni, Rodrigo, E-mail: Rodrigo.Bibiloni@agresearch.co.nz [Nestlé Research Centre, Nestec Ltd, Vers-chez-les-Blanc, CH-1000 Lausanne 26 (Switzerland); Schmitt, Bertrand, E-mail: bertrand.schmitt@rdls.nestle.com [Nestlé Research Centre, Nestec Ltd, Vers-chez-les-Blanc, CH-1000 Lausanne 26 (Switzerland); Reuteler, Gloria, E-mail: gloria.reuteler@rdls.nestle.com [Nestlé Research Centre, Nestec Ltd, Vers-chez-les-Blanc, CH-1000 Lausanne 26 (Switzerland); Brüssow, Harald, E-mail: harald.bruessow@rdls.nestle.com [Nestlé Research Centre, Nestec Ltd, Vers-chez-les-Blanc, CH-1000 Lausanne 26 (Switzerland)

    2013-09-01

    Phage therapy has a long tradition in Eastern Europe, where preparations are comprised of complex phage cocktails whose compositions have not been described. We investigated the composition of a phage cocktail from the Russian pharmaceutical company Microgen targeting Escherichia coli/Proteus infections. Electron microscopy identified six phage types, with numerically T7-like phages dominating over T4-like phages. A metagenomic approach using taxonomical classification, reference mapping and de novo assembly identified 18 distinct phage types, including 7 genera of Podoviridae, 2 established and 2 proposed genera of Myoviridae, and 2 genera of Siphoviridae. De novo assembly yielded 7 contigs greater than 30 kb, including a 147-kb Myovirus genome and a 42-kb genome of a potentially new phage. Bioinformatic analysis did not reveal undesired genes and a small human volunteer trial did not associate adverse effects with oral phage exposure. - Highlights: • We analyzed the composition of a commercial Russian phage cocktail. • The cocktail consists of at least 10 different phage genera. • No undesired genes were detected. • No adverse effects were seen upon oral application in a small human clinical trial.

  7. The Communist Party of China and Human Rights

    Institute of Scientific and Technical Information of China (English)

    YE XIAOWEN

    2011-01-01

    The Communist Party of China (CPC) has persistently fought for human rights since its founding in 1921.This is epitomized by the Party's success in promoting democracy,getting the Chinese nation to rally around it,uniting with religious believers and protecting human rights.

  8. The Effect of Vinpocetine on Human Cytochrome P450 Isoenzymes by Using a Cocktail Method

    Directory of Open Access Journals (Sweden)

    Lingti Kong

    2016-01-01

    Full Text Available Vinpocetine is a derivative of the alkaloid vincamine, which had been prescribed for chronic cerebral vascular ischemia and acute ischemic stroke or used as a dietary supplement for its several different mechanisms of biological activities. However, information on the cytochrome P450 (CYP enzyme-mediated drug metabolism has not been previously studied. The present study was performed to investigate the effects of vinpocetine on CYPs activity, and cocktail method was used, respectively. To evaluate the effects of vinpocetine on the activity of human CYP3A4, CYP2C9, CYP2C19, CYP2D6, and CYP2E1, human liver microsomes were utilized to incubate with the mixed CYPs probe substrates and the target components. The results indicate that vinpocetine exhibited weak inhibitory effect on the CYP2C9, where the IC50 value is 68.96 μM, whereas the IC50 values for CYP3A4, CYP2C19, CYP2D6, and CYP2E1 were all over range of 100 μM, which showed that vinpocetine had no apparent inhibitory effects on these CYPs. In conclusion, the results indicated that drugs metabolized by CYP2C9 coadministrated with vinpocetine may require attention or dose adjustment.

  9. Safety analysis of a Russian phage cocktail: from metagenomic analysis to oral application in healthy human subjects.

    Science.gov (United States)

    McCallin, Shawna; Alam Sarker, Shafiqul; Barretto, Caroline; Sultana, Shamima; Berger, Bernard; Huq, Sayeda; Krause, Lutz; Bibiloni, Rodrigo; Schmitt, Bertrand; Reuteler, Gloria; Brüssow, Harald

    2013-09-01

    Phage therapy has a long tradition in Eastern Europe, where preparations are comprised of complex phage cocktails whose compositions have not been described. We investigated the composition of a phage cocktail from the Russian pharmaceutical company Microgen targeting Escherichia coli/Proteus infections. Electron microscopy identified six phage types, with numerically T7-like phages dominating over T4-like phages. A metagenomic approach using taxonomical classification, reference mapping and de novo assembly identified 18 distinct phage types, including 7 genera of Podoviridae, 2 established and 2 proposed genera of Myoviridae, and 2 genera of Siphoviridae. De novo assembly yielded 7 contigs greater than 30 kb, including a 147-kb Myovirus genome and a 42-kb genome of a potentially new phage. Bioinformatic analysis did not reveal undesired genes and a small human volunteer trial did not associate adverse effects with oral phage exposure.

  10. Cleopatra's cocktail.

    Science.gov (United States)

    Jones, Prudence J

    2010-01-01

    Pliny the Elder's account of Cleopatra consuming a cocktail of vinegar and a pearl in order to win a bet with Antony was considered credible in the ancient world, but many modern scholars have relegated the anecdote to the realm of fantasy. This paper identifies possible reasons for this skepticism, including the visual tradition of the story and the belief that increasing concentration always increases reaction rate. Experiments reveal that, in the case of acetic acid and pearls, the concentration found in vinegar made from wine is ideal.

  11. A cocktail of humanized anti-pertussis toxin antibodies limits disease in murine and baboon models of whooping cough.

    Science.gov (United States)

    Nguyen, Annalee W; Wagner, Ellen K; Laber, Joshua R; Goodfield, Laura L; Smallridge, William E; Harvill, Eric T; Papin, James F; Wolf, Roman F; Padlan, Eduardo A; Bristol, Andy; Kaleko, Michael; Maynard, Jennifer A

    2015-12-01

    Despite widespread vaccination, pertussis rates are rising in industrialized countries and remain high worldwide. With no specific therapeutics to treat disease, pertussis continues to cause considerable infant morbidity and mortality. The pertussis toxin is a major contributor to disease, responsible for local and systemic effects including leukocytosis and immunosuppression. We humanized two murine monoclonal antibodies that neutralize pertussis toxin and expressed them as human immunoglobulin G1 molecules with no loss of affinity or in vitro neutralization activity. When administered prophylactically to mice as a binary cocktail, antibody treatment completely mitigated the Bordetella pertussis-induced rise in white blood cell counts and decreased bacterial colonization. When administered therapeutically to baboons, antibody-treated, but not untreated control animals, experienced a blunted rise in white blood cell counts and accelerated bacterial clearance rates. These preliminary findings support further investigation into the use of these antibodies to treat human neonatal pertussis in conjunction with antibiotics and supportive care.

  12. Finding your mate at a cocktail party: frequency separation promotes auditory stream segregation of concurrent voices in multi-species frog choruses.

    Directory of Open Access Journals (Sweden)

    Vivek Nityananda

    Full Text Available Vocal communication in crowded social environments is a difficult problem for both humans and nonhuman animals. Yet many important social behaviors require listeners to detect, recognize, and discriminate among signals in a complex acoustic milieu comprising the overlapping signals of multiple individuals, often of multiple species. Humans exploit a relatively small number of acoustic cues to segregate overlapping voices (as well as other mixtures of concurrent sounds, like polyphonic music. By comparison, we know little about how nonhuman animals are adapted to solve similar communication problems. One important cue enabling source segregation in human speech communication is that of frequency separation between concurrent voices: differences in frequency promote perceptual segregation of overlapping voices into separate "auditory streams" that can be followed through time. In this study, we show that frequency separation (ΔF also enables frogs to segregate concurrent vocalizations, such as those routinely encountered in mixed-species breeding choruses. We presented female gray treefrogs (Hyla chrysoscelis with a pulsed target signal (simulating an attractive conspecific call in the presence of a continuous stream of distractor pulses (simulating an overlapping, unattractive heterospecific call. When the ΔF between target and distractor was small (e.g., ≤3 semitones, females exhibited low levels of responsiveness, indicating a failure to recognize the target as an attractive signal when the distractor had a similar frequency. Subjects became increasingly more responsive to the target, as indicated by shorter latencies for phonotaxis, as the ΔF between target and distractor increased (e.g., ΔF = 6-12 semitones. These results support the conclusion that gray treefrogs, like humans, can exploit frequency separation as a perceptual cue to segregate concurrent voices in noisy social environments. The ability of these frogs to segregate

  13. Quality-controlled small-scale production of a well-defined bacteriophage cocktail for use in human clinical trials.

    Directory of Open Access Journals (Sweden)

    Maya Merabishvili

    Full Text Available We describe the small-scale, laboratory-based, production and quality control of a cocktail, consisting of exclusively lytic bacteriophages, designed for the treatment of Pseudomonas aeruginosa and Staphylococcus aureus infections in burn wound patients. Based on successive selection rounds three bacteriophages were retained from an initial pool of 82 P. aeruginosa and 8 S. aureus bacteriophages, specific for prevalent P. aeruginosa and S. aureus strains in the Burn Centre of the Queen Astrid Military Hospital in Brussels, Belgium. This cocktail, consisting of P. aeruginosa phages 14/1 (Myoviridae and PNM (Podoviridae and S. aureus phage ISP (Myoviridae was produced and purified of endotoxin. Quality control included Stability (shelf life, determination of pyrogenicity, sterility and cytotoxicity, confirmation of the absence of temperate bacteriophages and transmission electron microscopy-based confirmation of the presence of the expected virion morphologic particles as well as of their specific interaction with the target bacteria. Bacteriophage genome and proteome analysis confirmed the lytic nature of the bacteriophages, the absence of toxin-coding genes and showed that the selected phages 14/1, PNM and ISP are close relatives of respectively F8, phiKMV and phage G1. The bacteriophage cocktail is currently being evaluated in a pilot clinical study cleared by a leading Medical Ethical Committee.

  14. Simultaneous Determination of Cytochrome P450 Oxidation Capacity in Humans: A Review on the Phenotyping Cocktail Approach.

    Science.gov (United States)

    de Andrés, Fernando; LLerena, Adrián

    Intra- and/or inter-individual variability in drug response is mainly a result of either subtherapeutic or supratherapeutic plasma levels of the active drugs and their metabolites, with this variability mainly being influenced by differences in the rate of drug metabolism. Indeed, drug metabolism is largely determined by genetic polymorphism in the CYP enzymes, which are responsible for approximately 85% of the drug metabolism process. However, this genetic heterogeneity can accurately predict actual drug metabolizing capacity (oxidation phenotype) for some individuals: poor metabolizers (PMs), who cannot produce the drug metabolizing enzymes, and 20% of ultra-rapid metabolizers. According to EMA recommendations, phenotyping procedures for drug interaction studies and clinical research are therefore required to obtain actual data on the main CYP enzymes. With this purpose, cocktail phenotyping approaches give information on the activity of different CYPs in just one experiment. In this review, the issues related to the phenotyping of the main CYP enzymes are reviewed, and the current in vivo phenotyping cocktails are analysed: the sampling procedures, probe drugs utilized, analytical techniques and main applications are also discussed. Based on this analysis, a fully validated cocktail approach to measure the metabolic activity of the main CYP enzymes and drug transporters is still required. This novel approach should fulfil certain conditions: a faster and simpler analytical methodology to obtain information on several CYPs in one experiment, minimal sample amounts, and minimal doses of optimal probe drugs.

  15. The basel cocktail for simultaneous phenotyping of human cytochrome P450 isoforms in plasma, saliva and dried blood spots.

    Science.gov (United States)

    Donzelli, Massimiliano; Derungs, Adrian; Serratore, Maria-Giovanna; Noppen, Christoph; Nezic, Lana; Krähenbühl, Stephan; Haschke, Manuel

    2014-03-01

    Phenotyping cocktails use a combination of cytochrome P450 (CYP)-specific probe drugs to simultaneously assess the activity of different CYP isoforms. To improve the clinical applicability of CYP phenotyping, the main objectives of this study were to develop a new cocktail based on probe drugs that are widely used in clinical practice and to test whether alternative sampling methods such as collection of dried blood spots (DBS) or saliva could be used to simplify the sampling process. In a randomized crossover study, a new combination of commercially available probe drugs (the Basel cocktail) was tested for simultaneous phenotyping of CYP1A2, CYP2B6, CYP2C9, CYP2C19, CYP2D6 and CYP3A4. Sixteen subjects received low doses of caffeine, efavirenz, losartan, omeprazole, metoprolol and midazolam in different combinations. All subjects were genotyped, and full pharmacokinetic profiles of the probe drugs and their main metabolites were determined in plasma, dried blood spots and saliva samples. The Basel cocktail was well tolerated, and bioequivalence tests showed no evidence of mutual interactions between the probe drugs. In plasma, single timepoint metabolic ratios at 2 h (for CYP2C19 and CYP3A4) or at 8 h (for the other isoforms) after dosing showed high correlations with corresponding area under the concentration-time curve (AUC) ratios (AUC0-24h parent/AUC0-24h metabolite) and are proposed as simple phenotyping metrics. Metabolic ratios in dried blood spots (for CYP1A2 and CYP2C19) or in saliva samples (for CYP1A2) were comparable to plasma ratios and offer the option of minimally invasive or non-invasive phenotyping of these isoforms. This new combination of phenotyping probe drugs can be used without mutual interactions. The proposed sampling timepoints have the potential to facilitate clinical application of phenotyping but require further validation in conditions of altered CYP activity. The use of DBS or saliva samples seems feasible for phenotyping of the

  16. Staff Association Cocktail

    CERN Multimedia

    Staff Association

    2017-01-01

    The Staff Association has been organising for many years a cocktail with delegates of the Member States participating in Finance Committees of March and September. This cocktail is held at the end of the day, after the Finance Committee meeting. This direct and regular communication helps establish an ongoing contact between the Staff Association and CERN Member States and, more recently, the Associate Member States. Ambassadors of the CERN Staff Association, who are Members of the Personnel, have the opportunity to meet their national delegation in an informal and friendly atmosphere. These exchanges, facilitated by the use of the national language, allow the personnel via the Staff Association to express its ideas and positions on current affairs and fundamental issues, and also to hear about those of the delegations in return.

  17. Efficient generation of fully reprogrammed human iPS cells via polycistronic retroviral vector and a new cocktail of chemical compounds.

    Directory of Open Access Journals (Sweden)

    Zhonghui Zhang

    Full Text Available Direct reprogramming of human somatic cells into induced pluripotent stem (iPS cells by defined transcription factors (TFs provides great potential for regenerative medicine and biomedical research. This procedure has many challenges, including low reprogramming efficiency, many partially reprogrammed colonies, somatic coding mutations in the genome, etc. Here, we describe a simple approach for generating fully reprogrammed human iPS cells by using a single polycistronic retroviral vector expressing four human TFs in a single open reading frame (ORF, combined with a cocktail containing three small molecules (Sodium butyrate, SB431542, and PD0325901. Our results demonstrate that human iPS cells generated by this approach express human ES cells markers and exhibit pluripotency demonstrated by their abilities to differentiate into the three germ layers in vitro and in vivo. Notably, this approach not only provides a much faster reprogramming process but also significantly diminishes partially reprogrammed iPS cell colonies, thus facilitating efficient isolation of desired fully reprogrammed iPS cell colonies.

  18. A high-throughput inhibition screening of major human cytochrome P450 enzymes using an in vitro cocktail and liquid chromatography-tandem mass spectrometry.

    Science.gov (United States)

    Qin, Chong-Zhen; Ren, Xian; Tan, Zhi-Rong; Chen, Yao; Yin, Ji-Ye; Yu, Jing; Qu, Jian; Zhou, Hong-Hao; Liu, Zhao-Qian

    2014-02-01

    A sensitive and high-throughput inhibition screening liquid chromatography-mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous quantification of five probe metabolites (7-hydroxycoumarin, CYP2A6; 4-hydroxytolbutamide, CYP2C9; 4'-hydroxymephenytoin, CYP2C19; α-hydroxymetoprolol, CYP2D6; and 1-hydroxymidazolam, CYP3A4) for in vitro cytochrome P450 activity determination in human liver microsome and recombinant. All the metabolites and the internal standard, tramadol, were separated on a Waters 2695 series liquid chromatograph with a Phenomenex Luna C18 column (150 × 2.0 mm, 5 µm). Quality control samples and a positive control CYP inhibitor were included in the method. The IC50 values determined for typical CYP inhibitors were reproducible and in agreement with the literature. The method was selective and showed good accuracy (99.13-103.37%), and inter-day (RSD high-quality and -throughput cocktail provides suitable information in drug discovery and screening for new drug entities.

  19. Adhering maternal platelets can contribute to the cytokine and chemokine cocktail released by human first trimester villous placenta.

    Science.gov (United States)

    Blaschitz, A; Siwetz, M; Schlenke, P; Gauster, M

    2015-11-01

    Placental villous explant culture has been increasingly recognized as suitable model to study secretion of inflammatory and immune modulating factors by human placenta. Most of these factors likely derive from the syncytiotrophoblast, whereas extraplacental sources such as maternal peripheral blood cells are rarely considered. Due to their small size and absence of a nucleus, platelets adhering to perivillous fibrinoid of normal placenta are frequently ignored in routine immunohistochemistry. Here we demonstrate adhering maternal platelets on first trimester placental villi after explant culture and point out that platelet-derived factors must be considered when analyzing the inflammatory secretion profile of human placenta.

  20. Algorithms for Source Separation - with Cocktail Party Applications

    DEFF Research Database (Denmark)

    Olsson, Rasmus Kongsgaard

    2007-01-01

    of speech have been incorporated. Single-channel separation of speech is a particularly challenging signal processing task, where the purpose is to extract a number of speech signals from a single observed mixture. I present a few methods to obtain separation, which rely on the sparsity and structure......In this thesis, a number of possible solutions to source separation are suggested. Although they differ significantly in shape and intent, they share a heavy reliance on prior domain knowledge. Most of the developed algorithms are intended for speech applications, and hence, structural features...... of speech in a time-frequency representation. My own contributions are based on learning dictionaries for each speaker separately and subsequently applying a concatenation of these dictionaries to separate a mixture. Sparse decompositions required for the decomposition are computed using nonnegative matrix...

  1. Cocktail effects on biomarker responses in fish

    Energy Technology Data Exchange (ETDEWEB)

    Celander, Malin C., E-mail: malin.celander@zool.gu.se [University of Gothenburg, Department of Zoology, Box 463, SE-405 30 Gothenburg (Sweden)

    2011-10-15

    One of today's greatest challenges in environmental toxicology is to understand effects of mixture toxicity, commonly referred to as cocktail effects, in humans and in wildlife. Biomarker responses in fish are routinely used to assess exposure of anthropogenic chemicals in the aquatic environment. However, little is known about how cocktail effects affect these biomarker responses. For this reason, there is an obvious risk for misinterpretation of biomarker-data and this can have profound negative effects on stakeholder's decisions and actions, as well as on legislations and remediation-plans initiated in order to reduce exposure to certain chemicals. Besides, chemical safety-levels are traditionally based on experiences from lab-studies with single chemicals, which is unfortunate as a chemical can be more toxic when it is mixed with other chemicals, because of the cocktail effect. This review focuses on pharmacokinetic interactions between different classes of pollutants on detoxification mechanisms and how that affects two commonly used biomarkers in the aquatic environment: (1) induction of cytochrome P450 1A (CYP1A) that is mediated via activation of the arylhydrocarbon receptor (AhR), used to assess exposure to aromatic hydrocarbons; (2) induction of vitellogenin (VTG) that is mediated via activation of the estrogen receptor (ER), used to assess exposure to estrogenic chemicals. These responses can be either directly or indirectly affected by the presence of other classes of pollutants as a result of cocktail effects. For example, chemicals that inhibit the function of key metabolic enzymes and transporter pumps that are involved in elimination of AhR- and ER agonists, can result in bioaccumulation of aromatic hydrocarbons and estrogenic chemicals resulting in increased biomarker responses. This cocktail effect can lead to overestimation of the actual exposure pressure. On the contrary, induction of expression of key metabolic enzymes and transporter

  2. Phage therapy pharmacology phage cocktails.

    Science.gov (United States)

    Chan, Benjamin K; Abedon, Stephen T

    2012-01-01

    Phage therapy is the clinical or veterinary application of bacterial viruses (bacteriophages) as antibacterial "drugs." More generally, phages can be used as biocontrol agents against plant as well as foodborne pathogens. In this chapter, we consider the therapeutic use of phage cocktails, which is the combining of two or more phage types to produce more pharmacologically diverse formulations. The primary motivation for the use of cocktails is their broader spectra of activity in comparison to individual phage isolates: they can impact either more bacterial types or achieve effectiveness under a greater diversity of conditions. The combining of phages can also facilitate better targeting of multiple strains making up individual bacterial species or covering multiple species that might be responsible for similar disease states, in general providing, relative to individual phage isolates, a greater potential for presumptive or empirical treatment. Contrasting the use of phage banks, or even phage isolation against specific etiologies that have been obtained directly from patients under treatment, here we consider the utility as well as potential shortcomings associated with the use of phage cocktails as therapeutic antibacterial agents.

  3. Drug Cocktail Optimization in Chemotherapy of Cancer

    Science.gov (United States)

    Preissner, Saskia; Dunkel, Mathias; Hoffmann, Michael F.; Preissner, Sarah C.; Genov, Nikolai; Rong, Wen Wei; Preissner, Robert; Seeger, Karlheinz

    2012-01-01

    Background In general, drug metabolism has to be considered to avoid adverse effects and ineffective therapy. In particular, chemotherapeutic drug cocktails strain drug metabolizing enzymes especially the cytochrome P450 family (CYP). Furthermore, a number of important chemotherapeutic drugs such as cyclophosphamide, ifosfamide, tamoxifen or procarbazine are administered as prodrugs and have to be activated by CYP. Therefore, the genetic variability of these enzymes should be taken into account to design appropriate therapeutic regimens to avoid inadequate drug administration, toxicity and inefficiency. Objective The aim of this work was to find drug interactions and to avoid side effects or ineffective therapy in chemotherapy. Data sources and methods Information on drug administration in the therapy of leukemia and their drug metabolism was collected from scientific literature and various web resources. We carried out an automated textmining approach. Abstracts of PubMed were filtered for relevant articles using specific keywords. Abstracts were automatically screened for antineoplastic drugs and their synonyms in combination with a set of human CYPs in title or abstract. Results We present a comprehensive analysis of over 100 common cancer treatment regimens regarding drug-drug interactions and present alternatives avoiding CYP overload. Typical concomitant medication, e.g. antiemetics or antibiotics is a preferred subject to improvement. A webtool, which allows drug cocktail optimization was developed and is publicly available on http://bioinformatics.charite.de/chemotherapy. PMID:23236419

  4. Drug cocktail optimization in chemotherapy of cancer.

    Directory of Open Access Journals (Sweden)

    Saskia Preissner

    Full Text Available BACKGROUND: In general, drug metabolism has to be considered to avoid adverse effects and ineffective therapy. In particular, chemotherapeutic drug cocktails strain drug metabolizing enzymes especially the cytochrome P450 family (CYP. Furthermore, a number of important chemotherapeutic drugs such as cyclophosphamide, ifosfamide, tamoxifen or procarbazine are administered as prodrugs and have to be activated by CYP. Therefore, the genetic variability of these enzymes should be taken into account to design appropriate therapeutic regimens to avoid inadequate drug administration, toxicity and inefficiency. OBJECTIVE: The aim of this work was to find drug interactions and to avoid side effects or ineffective therapy in chemotherapy. DATA SOURCES AND METHODS: Information on drug administration in the therapy of leukemia and their drug metabolism was collected from scientific literature and various web resources. We carried out an automated textmining approach. Abstracts of PubMed were filtered for relevant articles using specific keywords. Abstracts were automatically screened for antineoplastic drugs and their synonyms in combination with a set of human CYPs in title or abstract. RESULTS: We present a comprehensive analysis of over 100 common cancer treatment regimens regarding drug-drug interactions and present alternatives avoiding CYP overload. Typical concomitant medication, e.g. antiemetics or antibiotics is a preferred subject to improvement. A webtool, which allows drug cocktail optimization was developed and is publicly available on http://bioinformatics.charite.de/chemotherapy.

  5. A SOF Damage Control Resuscitation Cocktail

    Science.gov (United States)

    2015-05-01

    resuscitation (DCR) cocktail for use by SOF’s that is capable of improving survival from polytrauma in austere settings. The cocktail components...components are tested in a combat-relevant swine polytrauma model of hemorrhagic shock with traumatic brain injury, free internal bleeding from an aortic...from polytrauma in austere settings. The cocktail components include Hextend for volume resuscitation and tissue perfusion, fibrinogen concentrate

  6. 26 CFR 1.276-1 - Disallowance of deductions for certain indirect contributions to political parties.

    Science.gov (United States)

    2010-04-01

    ... presentation, cocktail or other party, picnic, barbecue, sporting event, brunch, tea, supper, auction, bazaar... contributions to political parties. 1.276-1 Section 1.276-1 Internal Revenue INTERNAL REVENUE SERVICE... Disallowance of deductions for certain indirect contributions to political parties. (a) In general...

  7. Bacteriophage cocktail for biocontrol of Salmonella in dried pet food.

    Science.gov (United States)

    Heyse, Serena; Hanna, Leigh Farris; Woolston, Joelle; Sulakvelidze, Alexander; Charbonneau, Duane

    2015-01-01

    Human salmonellosis has been associated with contaminated pet foods and treats. Therefore, there is interest in identifying novel approaches for reducing the risk of Salmonella contamination within pet food manufacturing environments. The use of lytic bacteriophages shows promise as a safe and effective way to mitigate Salmonella contamination in various food products. Bacteriophages are safe, natural, highly targeted antibacterial agents that specifically kill bacteria and can be targeted to kill food pathogens without affecting other microbiota. In this study, we show that a cocktail containing six bacteriophages had a broadspectrum activity in vitro against a library of 930 Salmonella enterica strains representing 44 known serovars. The cocktail was effective against 95% of the strains in this tested library. In liquid culture dose-ranging experiments, bacteriophage cocktail concentrations of ≥10(8) PFU/ml inactivated more than 90% of the Salmonella population (10(1) to 10(3) CFU/ml). Dried pet food inoculated with a mixture containing equal proportions of Salmonella serovars Enteritidis (ATCC 4931), Montevideo (ATCC 8387), Senftenberg (ATCC 8400), and Typhimurium (ATCC 13311) and then surface treated with the six-bacteriophage cocktail (≥2.5 ± 1.5 × 10(6) PFU/g) achieved a greater than 1-log (P food that tested positive for Salmonella. Our results indicate that bacteriophage biocontrol of S. enterica in dried pet food is technically feasible.

  8. Your Golden Jubilee Party!

    CERN Multimedia

    2004-01-01

    http://www.cern.ch/cern50/ All staff are invited to the Golden Jubilee Staff Party on the evening of 17 September. To mark CERN's 50th anniversary, the Director-General would like to invite all the people working at CERN to celebrate the success of the organization. Your Golden Jubilee Party Friday 17 September 2004 at 16:00 Restaurant 1, Main Building Programme Speech by Director-General Toast in honour of CERN Cocktails and ambient music Video projections of five decades of CERN and at 18:00 ... A surprise music show. Your effort, hard work and enthusiasm have been the key to CERN's success. The first of the main events celebrating the organization's Golden Jubilee is for you, so come along and celebrate! Early closure of Restaurant No. 1 (bldg. 501, Meyrin site): Friday 17 September at 2:00 p.m. On Friday, 17 September, Restaurant No. 1 (NOVAE) will close at 2:00 p.m. owing to the Golden Jubilee party for the CERN staff. No dinner will be served that evening.

  9. Phage cocktails and the future of phage therapy.

    Science.gov (United States)

    Chan, Benjamin K; Abedon, Stephen T; Loc-Carrillo, Catherine

    2013-06-01

    Viruses of bacteria, known as bacteriophages or phages, were discovered nearly 100 years ago. Their potential as antibacterial agents was appreciated almost immediately, with the first 'phage therapy' trials predating Fleming's discovery of penicillin by approximately a decade. In this review, we consider phage therapy that can be used for treating bacterial infections in humans, domestic animals and even biocontrol in foods. Following an overview of the topic, we explore the common practice - both experimental and, in certain regions of the world, clinical - of mixing therapeutic phages into cocktails consisting of multiple virus types. We conclude with a discussion of the commercial and medical context of phage cocktails as therapeutic agents. In comparing off-the-shelf versus custom approaches, we consider the merits of a middle ground, which we deem 'modifiable'. Finally, we explore a regulatory framework for such an approach based on an influenza vaccine model.

  10. Memory Without Parties or Parties Without Memory?

    Directory of Open Access Journals (Sweden)

    Juan Mario Solís Delgadillo

    2011-01-01

    Full Text Available This paper explores the role of political parties in Argentina, Chile and Guatemala in relation to the implementation of public policies of memory after the return to democracy in each of these countries. To do this, we discuss the concept of memory and the problems of memorial obsession. We consider the uses and abuses of memory that human rights organizations manifest on the subject, and examine the work of the parties about the level of adaptation that allows claims of human rights movement to become matters of public policy.

  11. Publishing SNP genotypes of human embryonic stem cell lines: policy statement of the International Stem Cell Forum Ethics Working Party.

    Science.gov (United States)

    Knoppers, Bartha M; Isasi, Rosario; Benvenisty, Nissim; Kim, Ock-Joo; Lomax, Geoffrey; Morris, Clive; Murray, Thomas H; Lee, Eng Hin; Perry, Margery; Richardson, Genevra; Sipp, Douglas; Tanner, Klaus; Wahlström, Jan; de Wert, Guido; Zeng, Fanyi

    2011-09-01

    Novel methods and associated tools permitting individual identification in publicly accessible SNP databases have become a debatable issue. There is growing concern that current technical and ethical safeguards to protect the identities of donors could be insufficient. In the context of human embryonic stem cell research, there are no studies focusing on the probability that an hESC line donor could be identified by analyzing published SNP profiles and associated genotypic and phenotypic information. We present the International Stem Cell Forum (ISCF) Ethics Working Party's Policy Statement on "Publishing SNP Genotypes of Human Embryonic Stem Cell Lines (hESC)". The Statement prospectively addresses issues surrounding the publication of genotypic data and associated annotations of hESC lines in open access databases. It proposes a balanced approach between the goals of open science and data sharing with the respect for fundamental bioethical principles (autonomy, privacy, beneficence, justice and research merit and integrity).

  12. CombiCap: A novel drug formulation for the basel phenotyping cocktail.

    Science.gov (United States)

    Camblin, Marine; Berger, Benjamin; Haschke, Manuel; Krähenbühl, Stephan; Huwyler, Jörg; Puchkov, Maxim

    2016-10-15

    Phenotyping of cytochrome P450 isoenzymes is used for metabolic profiling. Phenotyping cocktails are usually administered as individual marketed products, which are not designed for diagnostic applications. Therefore, a formulation strategy was developed, which can be applied to any phenotyping cocktail. The formulation was validated in vitro and in vivo in human volunteers using caffeine, efavirenz, flurbiprofen, metoprolol, midazolam, and omeprazole (Basel Cocktail). Spray dried di-calcium phosphate particles (Fujicalin) were used as an inert drug carrier for probe drugs. All drugs were successfully loaded into Fujicalin by a solvent evaporation method. Mini-tablets were produced and demonstrated good physical characteristics, expected drug content and immediate release profiles for all drug formulations. Mini-tablets were introduced into a capsule (CombiCap) and used for a pilot study in human volunteers. Plasma samples were collected and analyzed by liquid chromatography and mass spectrometry. Plasma concentration ratios between the parent drugs and the respective metabolites were equivalent for the novel CombiCap formulation and individually dosed Basel Cocktail drugs. We conclude that the CombiCap formulation platform can be easily adopted for different types of phenotyping cocktails due to its scalable and modular design, which allows a simple and convenient combination of variable doses of different probe drugs. Copyright © 2016 Elsevier B.V. All rights reserved.

  13. "Super" Cocktails for Heavy Ion Testing

    Energy Technology Data Exchange (ETDEWEB)

    Johnson, Michael B; Johnson, Michael B.; McMahan, Margaret A.; Galloway, Michelle; Leitner, Daniela; Morel, James R.; Gimpel, ThomasL.; Ninemire, Brien F.; Siero, Reba; Thatcher, Raymond K.

    2007-07-21

    The 4.5 MeV/nucleon heavy ion cocktail at the 88-Inch Cyclotron has been expanded by incorporating beams from solid material to fill in the linear energy transfer curve. This supercocktail is available by special request and is useful when only normal incidence between the beam and the device under test is possible or desirable.

  14. Christmas party

    CERN Multimedia

    Staff Association

    2011-01-01

    The Christmas party organized by the Staff Association took place on Saturday 3 December. 200 children from 5 to 7 years old attended a magic show which filled them with wonder. Then they were served a snack in the Novae restaurant. Finally, just before their parents came to pick them up, Father Christmas gave them a present. The Staff Association wishes you a Merry Christmas and a Happy New Year!

  15. Appraising Party Cadres

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    An efficient cadre appraisal system guarantees cadres of the Communist Party of China will serve the people wholeheartedly According to the Progress in A China’s Human Rights in 2009 white paper,there were more than 2.9 million ethnic-minority cadres in China,accounting for 7.4 percent

  16. Ataxin-10 is part of a cachexokine cocktail triggering cardiac metabolic dysfunction in cancer cachexia

    Directory of Open Access Journals (Sweden)

    Michaela Schäfer

    2016-02-01

    Conclusions: As Ataxin-10 serum levels were also found to be elevated in human cachectic cancer patients, the identification of Ataxin-10 as part of a cachexokine cocktail now provides a rational approach towards personalized predictive, diagnostic and therapeutic measures in cancer cachexia.

  17. Development of a reference liquid scintillation cocktail

    CSIR Research Space (South Africa)

    Van Wyn Gaardt, WM

    2006-02-01

    Full Text Available of a scintillator dissolved in an organic solvent. The so-called scintillator solute is a material which emits a weak light flash or scintillation upon interaction with a certain quanta of radiation – the intensity of this scintillation being a... to prepare preliminary liquid scintillation cocktails. Figure 3: Maximum deviations observed between repeat measurements of the same source, displayed in red for Ultima Gold, green for XP, yellow for XI and blue for XPI. Figure 4: The counting...

  18. Bookworms and Party Animals: An Artificial Labour Market with Human and Social Capital Accumulation

    Science.gov (United States)

    Farhat, Daniel

    2014-01-01

    Data show that educated workers earn higher wages and are unemployed less often. Some researchers believe that education improves a worker's productivity (or "human capital"), making them more desirable on the job market, while others believe that it improves a worker's network (or "social capital"), giving them more…

  19. Bookworms and Party Animals: An Artificial Labour Market with Human and Social Capital Accumulation

    Science.gov (United States)

    Farhat, Daniel

    2014-01-01

    Data show that educated workers earn higher wages and are unemployed less often. Some researchers believe that education improves a worker's productivity (or "human capital"), making them more desirable on the job market, while others believe that it improves a worker's network (or "social capital"), giving them more…

  20. Tumour biology: Herceptin acts as an anti-angiogenic cocktail

    Science.gov (United States)

    Izumi, Yotaro; Xu, Lei; di Tomaso, Emmanuelle; Fukumura, Dai; Jain, Rakesh K.

    2002-03-01

    Malignant tumours secrete factors that enable them to commandeer their own blood supply (angiogenesis), and blocking the action of these factors can inhibit tumour growth. But because tumours may become resistant to treatments that target individual angiogenic factors by switching over to other angiogenic molecules, a cocktail of multiple anti-angiogenic agents should be more effective. Here we show that herceptin, a monoclonal antibody against the cell-surface receptor HER2 (for human epidermal growth factor receptor-2; ref. 4), induces normalization and regression of the vasculature in an experimental human breast tumour that overexpresses HER2 in mice, and that it works by modulating the effects of different pro- and anti-angiogenic factors. As a single agent that acts against multiple targets, herceptin, or drugs like it, may offer a simple alternative to combination anti-angiogenic treatments.

  1. The cosmic cocktail three parts dark matter

    CERN Document Server

    Freese, Katherine

    2014-01-01

    The ordinary atoms that make up the known universe-from our bodies and the air we breathe to the planets and stars-constitute only 5 percent of all matter and energy in the cosmos. The rest is known as dark matter and dark energy, because their precise identities are unknown. The Cosmic Cocktail is the inside story of the epic quest to solve one of the most compelling enigmas of modern science - what is the universe made of? - told by one of today's foremost pioneers in the study of dark matter. Blending cutting-edge science with her own behind-the-scenes insights as a leading researcher in the

  2. Cocktail of Theileria equi antigens for detecting infection in equines

    Directory of Open Access Journals (Sweden)

    Shimaa Abd El-Salam El-Sayed

    2015-12-01

    Conclusions: The ELISA technique using a cocktail formula of EMA-2 + Te 82 offers a practical and sensitive diagnostic tool for diagnosing T. equi infection in horses and using of this promising cocktail formula will be applicable for epidemiological surveys and will help control the infection in horses.

  3. Reduced responses of submucous neurons from irritable bowel syndrome patients to a cocktail containing histamine, serotonin, TNFα and tryptase (IBS-cocktail

    Directory of Open Access Journals (Sweden)

    Daniela eOstertag

    2015-12-01

    Full Text Available Background & Aims:Malfunctions of enteric neurons are believed to play an important role in the pathophysiology of irritable bowel syndrome (IBS. Our aim was to investigate whether neuronal activity in biopsies from IBS patients is altered in comparison to healthy controls (HC.Methods:Activity of human submucous neurons in response to electrical nerve stimulation and local application of nicotine or a mixture of histamine, serotonin, tryptase and TNF-α (IBS-cocktail was recorded in biopsies from 17 HC and 35 IBS patients with the calcium-sensitive-dye Fluo-4 AM. The concentrations of the mediators resembeled those found in biopsy supernatants or blood. Neuronal activity in guinea-pig submucous neurons was studied with the voltage-sensitive-dye di-8-ANEPPS. Results:Activity in submucous ganglia in response to nicotine or electrical nerve stimulation was not different between HC and IBS patients (P=0.097 or P=0.448. However, the neuronal response after application of the IBS-cocktail was significantly decreased (P=0.039 independent of whether diarrhea (n=12, constipation (n=5 or bloating (n=5 was the predominant symptom. In agreement with this we found that responses of submucous ganglia conditioned by overnight incubation with IBS mucosal biopsy supernatant to spritz application of this supernatant was significantly reduced (P=0.019 when compared to incubation with HC supernatant.Conclusion:We demonstrated for the first time reduced neuronal responses in mucosal IBS biopsies to an IBS mediator cocktail. While excitability to classical stimuli of enteric neurons was comparable to HC, the activation by the IBS-cocktail was decreased. This was very likely due to desensitization to mediators constantly released by mucosal and immune cells in the gut wall of IBS patients.

  4. Flumazenil, naloxone and the 'coma cocktail'.

    Science.gov (United States)

    Sivilotti, Marco L A

    2016-03-01

    Flumazenil and naloxone are considered to be pharmacologically ideal antidotes. By competitive binding at the molecular target receptors, they are highly specific antagonists of two important drug classes, the benzodiazepines and opioids, respectively. Both antidotes enjoy rapid onset and short duration after parenteral administration, are easily titrated and are essentially devoid of agonist effects. Yet only naloxone is widely used as a component of the 'coma cocktail', a sequence of empirical treatments to correct altered mental status, while experts discourage the use of flumazenil for such patients. This review contrasts the history, indications, published evidence and novel applications for each antidote in order to explain this disparity in the clinical use of these 'ideal' antidotes.

  5. My Birthday Party

    Institute of Scientific and Technical Information of China (English)

    刘世一; 张光远

    2005-01-01

    Birthday party is a celebration for one's grown-up.Every year people celebrate their birthday in different ways.Young teenagers just like us are eager to have parties,but some old people are afraid to have their birthday party.Maybe they don't hope to be older.But I think birthday party is a joyful day for me.

  6. Cocoa Fermentations Conducted with a Defined Microbial Cocktail Inoculum

    OpenAIRE

    Schwan, Rosane Freitas

    1998-01-01

    Cocoa fermentations were performed in wooden boxes under the following four experimental regimens: beans naturally fermented with wild microflora; aseptically prepared beans with no inoculum; and beans inoculated with a defined cocktail containing microorganisms at a suitable concentration either at zero time or by using phased additions at appropriate times. The cocktail used consisted of a yeast, Saccharomyces cerevisiae var. chevalieri, two lactic acid bacterial species, Lactobacillus lact...

  7. A Performance Comparison of Nine Selected Liquid Scintillation Cocktails

    Energy Technology Data Exchange (ETDEWEB)

    Verrezen, F.; Loots, H.; Hurtgen, Ch.

    2008-06-15

    In the selection of a suitable liquid scintillation (LSC) cocktail, the primary aspects taken into consideration are overall cocktail performance and specific laboratory needs. Overall performance of 9 selected, commercially available LSC cocktails was assessed by studying parameters of importance for the requirements of the Laboratory for Low Level Radioactivity Measurements of SCK-CEN: sample load capacity, sample compatibility, influence of sample load on counting efficiency, background count rate, figure of merit, quench resistance, sample stability and alpha/beta separation characteristics. The cocktails tested were EcoscintA, Insta Gel Plus, OptiPhase Hisafe3, OptiPhase Trisafe, Ready Gel, SafeScint 1:1, Ultima Gold, Ultima Gold LLT, and Ultima Gold XR. For the data acquisition a Packard TriCarb Model 1900CA and a Quantulus 1220 liquid scintillation counter is used. All samples were prepared in either 20 mL low potassium, borosilicate glass vials or 20 mL high density, polyethylene vials. The aim of this study was to determine a single cocktail that best suits all measurement requirements of the liquid scintillation laboratory at SCK-CEN for the determination of low levels of radioactivity in biological and environmental samples. As a conclusion, Optiphase HiSafe 3 was confirmed to be the optimal cocktail for the laboratory.

  8. Effects of age on electrophysiological correlates of speech processing in a dynamic cocktail-party situation

    Directory of Open Access Journals (Sweden)

    Stephan eGetzmann

    2015-09-01

    Full Text Available Successful speech perception in multi-speaker environments depends on auditory scene analysis, comprising auditory object segregation and grouping, and on focusing attention toward the speaker of interest. Changes in speaker settings (e.g., in speaker position require object re-selection and attention re-focusing. Here, we tested the processing of changes in a realistic multi-speaker scenario in younger and older adults, employing a speech-perception task and event-related potential (ERP measures. Sequences of short words (combinations of company names and values were simultaneously presented via four loudspeakers at different locations, and the participants responded to the value of a target company. Voice and position of the speaker of the target information were kept constant for a variable number of trials and then changed. Relative to the pre-change level, changes caused higher error rates, and more so in older than younger adults. The ERP analysis revealed stronger fronto-central N2 and N400 components in younger adults, suggesting a more effective inhibition of concurrent speech stimuli and enhanced language processing. The difference ERPs (post-change minus pre-change indicated a change-related N400 and late positive complex (LPC over parietal areas in both groups. Only the older adults showed an additional frontal LPC, suggesting increased allocation of attentional resources after changes in speaker settings. In sum, changes in speaker settings are critical events for speech perception in multi-speaker environments. Especially older persons show deficits that could be based on less flexible inhibitory control and increased distraction.

  9. Switching in the Cocktail Party: Exploring Intentional Control of Auditory Selective Attention

    Science.gov (United States)

    Koch, Iring; Lawo, Vera; Fels, Janina; Vorlander, Michael

    2011-01-01

    Using a novel variant of dichotic selective listening, we examined the control of auditory selective attention. In our task, subjects had to respond selectively to one of two simultaneously presented auditory stimuli (number words), always spoken by a female and a male speaker, by performing a numerical size categorization. The gender of the…

  10. Envelope Responses in Single-Trial EEG Indicate Attended Speaker in a Cocktail Party

    Science.gov (United States)

    2013-06-20

    users to modulate their brain activity, such as motor rhythms , in order to signal intent [13], but these often require considerable training. Other...a failure to learn how to modulate certain brain rhythms , but rather on differences in the robustness of the stimulus-related signals of interest...monaural and dichotic listening. Journal of Neurophysiology 107 78–89 [11] Horton C, D’Zmura M and Srinivasan R 2013 Suppression of Competing Speech

  11. The cocktail-party problem revisited : early processing and selection of multi-talker speech

    NARCIS (Netherlands)

    Bronkhorst, A.W.

    2015-01-01

    How do we recognize what one person is saying when others are speaking at the same time? This review summarizes widespread research in psychoacoustics, auditory scene analysis, and attention, all dealing with early processing and selection of speech, which has been stimulated by this question.

  12. Envelope Responses in Single-trial EEG Indicate Attended Speaker in a Cocktail Party

    Science.gov (United States)

    2014-06-25

    visuospatial attention indexed by retinotopically specific alpha-band electroencephalography increases over occipital cortex J. Neurosci. 20 RC63 pp...1–6 [19] Thut G, Nietzel A, Brandt S A and Pascual-Leone A 2006 Alpha-band electroencephalographic activity over occipital cortex indexes visuospatial

  13. Kalman filter for speech enhancement in cocktail party scenarios using a codebook-based approach

    DEFF Research Database (Denmark)

    Kavalekalam, Mathew Shaji; Christensen, Mads Græsbøll; Gran, Fredrik;

    2016-01-01

    Enhancement of speech in non-stationary background noise is a challenging task, and conventional single channel speech enhancement algorithms have not been able to improve the speech intelligibility in such scenarios. The work proposed in this paper investigates a single channel Kalman filter based...

  14. Multi-exon Skipping Using Cocktail Antisense Oligonucleotides in the Canine X-linked Muscular Dystrophy.

    Science.gov (United States)

    Miskew Nichols, Bailey; Aoki, Yoshitsugu; Kuraoka, Mutsuki; Lee, Joshua J A; Takeda, Shin'ichi; Yokota, Toshifumi

    2016-05-24

    Duchenne muscular dystrophy (DMD) is one of the most common lethal genetic diseases worldwide, caused by mutations in the dystrophin (DMD) gene. Exon skipping employs short DNA/RNA-like molecules called antisense oligonucleotides (AONs) that restore the reading frame and produce shorter but functional proteins. However, exon skipping therapy faces two major hurdles: limited applicability (up to only 13% of patients can be treated with a single AON drug), and uncertain function of truncated proteins. These issues were addressed with a cocktail AON approach. While approximately 70% of DMD patients can be treated by single exon skipping (all exons combined), one could potentially treat more than 90% of DMD patients if multiple exon skipping using cocktail antisense drugs can be realized. The canine X-linked muscular dystrophy (CXMD) dog model, whose phenotype is more similar to human DMD patients, was used to test the systemic efficacy and safety of multi-exon skipping of exons 6 and 8. The CXMD dog model harbors a splice site mutation in intron 6, leading to a lack of exon 7 in dystrophin mRNA. To restore the reading frame in CXMD requires multi-exon skipping of exons 6 and 8; therefore, CXMD is a good middle-sized animal model for testing the efficacy and safety of multi-exon skipping. In the current study, a cocktail of antisense morpholinos targeting exon 6 and exon 8 was designed and it restored dystrophin expression in body-wide skeletal muscles. Methods for transfection/injection of cocktail oligos and evaluation of the efficacy and safety of multi-exon skipping in the CXMD dog model are presented.

  15. Heavy ion cocktail beams at the 88 inch Cyclotron

    Energy Technology Data Exchange (ETDEWEB)

    Leitner, Daniela; McMahan, Margaret A.; Argento, David; Gimpel, Thomas; Guy, Aran; Morel, James; Siero, Christine; Thatcher, Ray; Lyneis, Claude M.

    2002-09-03

    Cyclotrons in combination with ECR ion sources provide the ability to accelerate ''cocktails'' of ions. A cocktail is a mixture of ions of near-identical mass-to-charge (m/q) ratio. The different ions cannot be separated by the injector mass-analyzing magnet and are tuned out of the ion source together. The cyclotron then is utilized as a mass analyzer by shifting the accelerating frequency. This concept was developed soon after the first ECR ion source became operational at the 88-Inch Cyclotron and has since become a powerful tool in the field of heavy ion radiation effects testing. Several different ''cocktails'' at various energies are available at the 88-Inch cyclotron for radiation effect testing, covering a broad range of linear energy transfer and penetration depth. Two standard heavy ion cocktails at 4.5 MeV/nucleon and 10 MeV/nucleon have been developed over the years containing ions from boron to bismuth. Recently, following requests for higher penetration depths, a 15MeV/nucleon heavy ion cocktail has been developed. Up to nine different metal and gaseous ion beams at low to very high charge states are tuned out of the ion source simultaneously and injected together into the cyclotron. It is therefore crucial to balance the ion source very carefully to provide sufficient intensities throughout the cocktail. The paper describes the set-up and tuning of the ion source for the various heavy ion cocktails.

  16. Party brands and voting

    DEFF Research Database (Denmark)

    Nielsen, Sigge Winther; Larsen, Martin Vinæs

    2014-01-01

    the question: Do party brands influence voting behavior? Currently, we do not know because the two research fields of voting behavior and party brands are currently not explicitly linked. Traditionally, the study of voting behavior has gained powerful insights from concepts such as cleavage structure, party...

  17. Partying as Everyday Life

    DEFF Research Database (Denmark)

    Demant, Jakob; Østergaard, Jeanette

    2007-01-01

      This article investigates what partying means to Danish adolescents aged 14-16. A new theoretical approach to teenage partying is suggested. It combines the structural anthropological tradition of analysing partying and use of alcohol as a rite de passage with a phenomenological perspective which...

  18. Museums & Mermaids: Bringing Climate Literacy to the Party

    Science.gov (United States)

    Tillinger, D.

    2014-12-01

    If science is for everyone, then it needs to be taught in environments that are welcoming to people who may not feel at home in a traditional classroom. A team of scientists and educators at the American Museum of Natural History have developed a new course, Our Earth's Future, which prepares participants to contribute intelligently and fluently to informal "cocktail party" conversations about climate and climate change. The course, taught after hours at the museum, culminates in an actual cocktail party in one of the museum's halls where participants can practice their skills. Participants' knowledge of climate change and attitudes towards climate change were quantitatively and qualitatively assessed before and after the two five-week course sessions.Climate literacy can also be included in existing events that are not obviously science-focused. Venues such as festivals, galleries, and underground art parties may be willing to broaden their definition of culture to include science - but only if they are asked. Given the increase in public discourse around the topic of climate change, there is an opportunity to reach people who wouldn't attend a formal science lecture, but might attend a film screening or a fundraiser that had some scientific content. Qualitative audience assessments done after the annual "Mermaid Lagoon," a fundraiser for ocean related causes that includes a scientific segment along with dance and theatrical performances, show increased enthusiasm and support for climate science when it is presented in a relevant, fun, and non-intimidating manner.

  19. the doctrine of party autonomy in international commercial arbitration

    African Journals Online (AJOL)

    RAYAN_

    1. INTRODUCTION. Conflict is unavoidable in human relationships. ..... efficiency and fairness in the dispute resolution process while allowing parties to exercise ..... evolved to act as checks and balances on the principle of party autonomy,.

  20. About green political parties

    Directory of Open Access Journals (Sweden)

    Orlović Slobodan P.

    2015-01-01

    Full Text Available In this work the author refers to some legal and political questions in connection with green political parties. Those questions cover: the ideology of green political parties, their number and influence, both in general and in Serbia. The first part of work is generally speaking about political parties - their definition, ideology, role and action. Main thesis in this work is that green political parties, by their appearance, were something new on the political scene. But quickly, because of objective and subjective reasons, they were changing original ideas and were beginning to resemble to all other political parties. In this way, they lost their vanguard and political alternativeness.

  1. Gendering transnational party politics

    DEFF Research Database (Denmark)

    Kantola, Johanna; Rolandsen-Agustín, Lise

    2016-01-01

    In this article, we analyze transnational party politics in the European Union from a gender perspective. This is a subject that has been neglected both by mainstream European studies on party politics and by gender scholars who work on political parties. Drawing on the insights of these two...... research traditions, we build toward an analytical framework to study gender and transnational party politics. Our empirical analysis focuses on two policy issues, the economic crisis and the sexual and reproductive health and rights, analyzing European Parliament reports, debates and voting on the issues...... right axis and, at the same time, internal divisions within party groups affect policy output....

  2. Party member recruitment and the representativeness of party membership

    DEFF Research Database (Denmark)

    Kosiara-Pedersen, Karina

    Party membership figures are in decline. However, parties are still recruiting party members. Are there any differences in the social and political characteristics of old and new party members? If there is a difference, are new party members more or less representative for party voters than old...

  3. Comparative analysis of two and three marker immunohistochemical cocktails in the diagnosis of prostata carcinoma

    DEFF Research Database (Denmark)

    Dabir, Parag Deepak; Ottosen, Peter; Hamilton-Dutoit, Stephen Jacques;

    2013-01-01

    Comparative analysis of two and three marker immunohistochemical cocktails in the diagnosis of prostata carcinoma......Comparative analysis of two and three marker immunohistochemical cocktails in the diagnosis of prostata carcinoma...

  4. Comparative analysis of two and three marker immunohistochemical cocktails in the diagnosis of prostata carcinoma

    DEFF Research Database (Denmark)

    Dabir, Parag Deepak; Ottosen, Peter; Hamilton-Dutoit, Stephen Jacques;

    2012-01-01

    Comparative analysis of two and three marker immunohistochemical cocktails in the diagnosis of prostata carcinoma......Comparative analysis of two and three marker immunohistochemical cocktails in the diagnosis of prostata carcinoma...

  5. Party brands and voting

    DEFF Research Database (Denmark)

    Nielsen, Sigge Winther; Larsen, Martin Vinæs

    2014-01-01

    Some scholars argue that Western societies have seen a decreasing impact of voting behavior based on cleavages and party identifications. Equally, issue ownership voting is seemingly not increasing its relevance by filling this gap. From this departure we seek out an alternative variable by posing...... the question: Do party brands influence voting behavior? Currently, we do not know because the two research fields of voting behavior and party brands are currently not explicitly linked. Traditionally, the study of voting behavior has gained powerful insights from concepts such as cleavage structure, party...... heuristics and voting models. Next, the article measures the brand value of Danish parties by utilizing a representative association analysis. Finally, this measure is used to conduct the very first empirical analysis of a party brand's effect on voting behavior. Overall, the primary finding demonstrates...

  6. ‘Ireland’s Largest Live Cocktail Challenge Attracts over 70 Mixologists’

    OpenAIRE

    2014-01-01

    The 2nd DIT Monin Cocktail Challenge took place on Thursday November 20th 2014, this initiative between the DIT Bar Management Studies and Monin attracted 70 mixologists. Prizes were awarded for the top non-alcohol cocktails presented and best technical performance. All cocktails were based on Monin premium syrups and purees, points were awarded for the appearance, aroma, taste and the commercial appeal of the cocktails. Mr. James Murphy (Competition Co-ordinator, DIT Lecturer, Bar Management...

  7. An automated, high-throughput, 384 well Cytochrome P450 cocktail IC50 assay using a rapid resolution LC-MS/MS end-point.

    Science.gov (United States)

    Youdim, Kuresh A; Lyons, Richard; Payne, Leon; Jones, Barry C; Saunders, Kenneth

    2008-09-10

    The current study focused on the development of an automated IC50 cocktail assay in a miniaturized 384 well assay format. This was developed in combination with a significantly shorter high pressure liquid chromatography (HPLC) separation and liquid chromatography-mass spectrometry (LC-MS/MS) run-time; than those currently reported in the literature. The 384-well assay used human liver microsomes in conjunction with a cocktail of probe substrates metabolized by the five major CYPs (tacrine for CYP1A2, diclofenac for CYP2C9, (S)-mephenytoin for CYP2C19, dextromethorphan for CYP2D6 and midazolam for CYP3A4). To validate the usefulness of the automated and analytical methodologies, IC50 determinations were performed for a series of test compounds known to exhibit inhibition across these five major P450s. Eight compounds (sertraline, disulfuram, ticlopidine fluconazole, fluvoxamine, ketoconazole, miconazole, paroxetine, flunitrazepam) were studied as part of a cocktail assay, and against each CYPs individually. The data showed that the IC50s generated with cocktail incubations did not differ to a great extent from those obtained in the single probe experiments and hence unlikely to significantly influence the predicted clinical DDI risk. In addition the present method offered a significant advantage over some of the existing cocktail analytical methodology in that separation can be achieved with run times as short as 1 min without compromising data integrity. Although numerous studies have been reported to measure CYP inhibition in a cocktail format the need to support growing discovery libraries not only relies on higher throughput assays but quicker analytical run times. The current study reports a miniaturized high-throughput cocktail IC50 assay, in conjunction with a robust, rapid resolution LC-MS/MS end-point offered increased sample throughput without compromising analytical sensitivity or analyte resolution.

  8. Cocktail of Theileria equi antigens for detecting infection in equines

    Institute of Scientific and Technical Information of China (English)

    Shimaa; Abd; El-Salam; El-Sayed; Mohamed; Abdo; Rizk; Mohamed; Alaa; Terkawi; Ahmed; Mousa; El; Said; El; Shirbini; El; Said; Gehad; Elsayed; Mohamed; Fouda; Naoaki; Yokoyama; Ikuo; Igarashi

    2015-01-01

    Objective:To use two diagnostic antigens belonging to the frequently associated in Theileria domain,Theileria equi(T.equi)protein 82(Te 82)and T.equi 104 k Da microneme-rhoptry antigen precursor(Te 43),to diagnose T.equi infection in horses as compared with equi merozoite antigen-2(EMA-2).Methods:In the current study,we applied a cocktail-ELISA containing two antigens(EMA-2+Te 82)to diagnose T.equi infection either in experimentally infected horses or in field infection.Results:Our findings have revealed that a cocktail formula of EMA-2+Te 82 provided a more practical and sensitive diagnostic candidate for diagnosing T.equi infection in horses as compared with Te 82 or Te 43 alone.Conclusions:The ELISA technique using a cocktail formula of EMA-2+Te 82 offers a practical and sensitive diagnostic tool for diagnosing T.equi infection in horses and using of this promising cocktail formula will be applicable for epidemiological surveys and will help control the infection in horses.

  9. Cocktail of Theileria equi antigens for detecting infection in equines

    Institute of Scientific and Technical Information of China (English)

    Shimaa Abd El-Salam El-Sayed; Mohamed Abdo Rizk; Mohamed Alaa Terkawi; Ahmed Mousa; El Said El Shirbini El Said; Gehad Elsayed; Mohamed Fouda; Naoaki Yokoyama; Ikuo Igarashi

    2015-01-01

    Objective: To use two diagnostic antigens belonging to the frequently associated in Theileria domain, Theileria equi (T. equi) protein 82 (Te 82) and T. equi 104 kDa microneme-rhoptry antigen precursor (Te 43), to diagnose T. equi infection in horses as compared with equi merozoite antigen-2 (EMA-2). Methods: In the current study, we applied a cocktail-ELISA containing two antigens (EMA-2+Te 82) to diagnose T. equi infection either in experimentally infected horses or in field infection. Results: Our findings have revealed that a cocktail formula of EMA-2+Te 82 provided a more practical and sensitive diagnostic candidate for diagnosing T. equi infection in horses as compared with Te 82 or Te 43 alone. Conclusions: The ELISA technique using a cocktail formula of EMA-2+Te 82 offers a practical and sensitive diagnostic tool for diagnosing T. equi infection in horses and using of this promising cocktail formula will be applicable for epidemiological surveys and will help control the infection in horses.

  10. Training to Increase Safe Tray Carrying among Cocktail Servers

    Science.gov (United States)

    Scherrer, Megan D.; Wilder, David A.

    2008-01-01

    We evaluated the effects of training on proper carrying techniques among 3 cocktail servers to increase safe tray carrying on the job and reduce participants' risk of developing musculoskeletal disorders. As participants delivered drinks to their tables, their finger, arm, and neck positions were observed and recorded. Each participant received…

  11. Molecular Characterization of the Monoclonal Antibodies Composing ZMAb: A Protective Cocktail Against Ebola Virus

    Science.gov (United States)

    Audet, Jonathan; Wong, Gary; Wang, Han; Lu, Guangwen; Gao, George F.; Kobinger, Gary; Qiu, Xiangguo

    2014-01-01

    Ebola virus (EBOV) causes severe viral hemorrhagic fever in humans and non-human primates, with a case fatality rate of up to 88% in human outbreaks. Over the past 3 years, monoclonal antibody (mAb) cocktails have demonstrated high efficacy as treatments against EBOV infection. One such cocktail is ZMAb, which consists of three mouse antibodies, 1H3, 2G4, and 4G7. Here, we present the epitope binding properties of mAbs 1H3, 2G4, and 4G7. We showed that these antibodies have different variable region sequences, suggesting that the individual mAbs are not clonally related. All three antibodies were found to neutralize EBOV variant Mayinga. Additionally, 2G4 and 4G7 were shown to cross-inhibit each other in vitro and select for an escape mutation at the same position on the EBOV glycoprotein (GP), at amino acid 508. 1H3 selects an escape mutant at amino acid 273 on EBOV GP. Surface plasmon resonance studies showed that all three antibodies have dissociation constants on the order of 10−7. In combination with previous studies evaluating the binding sites of other protective antibodies, our results suggest that antibodies targeting the GP1-GP2 interface and the glycan cap are often selected as efficacious antibodies for post-exposure interventions against EBOV. PMID:25375093

  12. Political Parties and Innovation

    DEFF Research Database (Denmark)

    Bischoff, Carina Saxlund; Christiansen, Flemming Juul

    2017-01-01

    Public innovation and political parties are usually not studied together. Given the key position parties hold in representative democracies, it is somewhat odd that their influence on public innovation has not been explored. We propose to open this line of inquiry and introduce a typology...... that highlights four avenues for studying the links between public innovation and political parties: linkage, programme, interaction and policy. We use the typology to discuss relevant themes and empirical examples in existing literature and to formulate of a number of hypotheses about innovation of political...... parties themselves as well their impact on potentially innovative public decisions. One major expectation is that hierarchical parties with centralized leadership make more efficient decisions but that sustainable innovation outcomes promoted by collaborative efforts are easier to obtain for decentralized...

  13. CONSIDERATIONS ON POLITICAL PARTIES

    Directory of Open Access Journals (Sweden)

    Erhard NICULESCU

    2015-07-01

    Full Text Available Political parties are the engine of social life, without which citizens would not be able to express political will. According to the law of political parties, they fulfill a public mission guaranteed by the Constitution. That is why, considering the subject very current, we considered it important to analyze in this study, in the notion of political pluralism: the concept of political party but also its constitutional dimension. To this end, the study shows the view of the doctrine combined with the jurisprudence of the Constitutional Court.

  14. Emergence of Ebola Virus Escape Variants in Infected Nonhuman Primates Treated with the MB-003 Antibody Cocktail.

    Science.gov (United States)

    Kugelman, Jeffrey R; Kugelman-Tonos, Johanny; Ladner, Jason T; Pettit, James; Keeton, Carolyn M; Nagle, Elyse R; Garcia, Karla Y; Froude, Jeffrey W; Kuehne, Ana I; Kuhn, Jens H; Bavari, Sina; Zeitlin, Larry; Dye, John M; Olinger, Gene G; Sanchez-Lockhart, Mariano; Palacios, Gustavo F

    2015-09-29

    MB-003, a plant-derived monoclonal antibody cocktail used effectively in treatment of Ebola virus infection in non-human primates, was unable to protect two of six animals when initiated 1 or 2 days post-infection. We characterized a mechanism of viral escape in one of the animals, after observation of two clusters of genomic mutations that resulted in five nonsynonymous mutations in the monoclonal antibody target sites. These mutations were linked to a reduction in antibody binding and later confirmed to be present in a viral isolate that was not neutralized in vitro. Retrospective evaluation of a second independent study allowed the identification of a similar case. Four SNPs in previously identified positions were found in this second fatality, suggesting that genetic drift could be a potential cause for treatment failure. These findings highlight the importance selecting different target domains for each component of the cocktail to minimize the potential for viral escape.

  15. Tea Party Engineer

    National Research Council Canada - National Science Library

    Mark Matthews

    2013-01-01

      [...]Massie, a Tea Party enthusiast and fan of both Ron and Rand Paul, has already made his presence felt on the House floor, bucking GOP leaders by voting against John Boehner's re-election as speaker...

  16. Large-scale preparation of human anti-third-party veto cytotoxic T lymphocytes depleted of graft-versus-host reactivity: a new source for graft facilitating cells in bone marrow transplantation.

    Science.gov (United States)

    Aviner, Shraga; Yao, Xin; Krauthgamer, Rita; Gan, Yehudit; Goren-Arbel, Rinat; Klein, Tirza; Tabilio, Antonio; McMannis, John D; Champlin, Richard; Martelli, Massimo F; Bachar-Lustig, Esther; Reisner, Yair

    2005-06-01

    Induction of donor type chimerism in mildly prepared hosts without graft-versus-host disease (GvHD) is a most desirable goal in bone morrow transplantation. We have recently demonstrated in a mouse model that donor veto cytotoxic T lymphocytes (CTLs) can facilitate the induction of donor type chimerism in sublethally irradiated recipients without causing GvHD if they are effectively depleted of alloreactivity against host cells by means of stimulation against a third party. We extend this approach to human cells, by preparing CTLs in two major steps: primary culture in the absence of interleukin 2, leading to death by neglect of antihost clones, and addition of interleukin 2 and subsequent dilution of antihost clones as a consequence of the expansion of the anti-third-party clones. CTLs prepared in this way specifically suppress host cytotoxic T cells directed against antigens of the donor, but not against fourth-party antigens, as demonstrated in a standard (51)Cr release assay. We conclude that human anti-third-party CTLs afford a new source of veto cells that are depleted of potential graft-versus-host-reactive clones. The cells generated by this approach could potentially be used to facilitate engraftment of allogeneic hematopoietic stem cells.

  17. DEVELOPMENT OF POLITICAL PARTIES AND PARTY FUNDING: MODELS AND CHARACTERISTICS

    Directory of Open Access Journals (Sweden)

    Hrvoje MATAKOVIC

    2015-03-01

    Full Text Available The first modern political parties were formed at the end of the 18th century and have, from those times up to now, undergone 4 developing phases; each of the phases is bound to ideal-type political party model: cadre parties, mass parties, catch-all parties and cartel parties. Each of these party models differentiates in various characteristics: party foundation, number of members, and way of leading the election campaigns, but also in ways of financing. This paper describes the above mentioned 4 phases of political parties’ development and 4 phases of parties' finances development; it will be analysed in detail positive and negative sides of each of the models of party financing.

  18. MixPlore: A Cocktail-Based Media Performance Using Tangible User Interfaces

    Science.gov (United States)

    Lee, Zune; Chang, Sungkyun; Lim, Chang Young

    This paper presents MixPlore, a framework for a cocktail-based live media performance. It aims to maximize the pleasure of mixology, presenting the cocktail as a plentiful art medium where people can fully enjoy new synesthetic contents by the integration of bartending and musical creation. For this, we fabricated Tangible User Interfaces (TUIs): tin, glass, muddler, and costume display, etc. The basic idea of performance and music composition is to follow the process of making cocktails. At the end of every repertoire, the performer provides the resultant 'sonic cocktails' to audience.

  19. Terrorism and Political Parties

    DEFF Research Database (Denmark)

    Bourne, Angela

    In the paper I address the empirical puzzle arising from different responses by political authorities in Spain and the UK to the existence of political parties integrated in the terrorist groups Euskadi Ta Askatasuna (ETA, Basque Homeland and Freedom) and the Irish Republican Army (IRA). More...... for realization of free speech rights and representation is also emphasized, and where proscription is seen as inimical to resolution of conflict underpinning violence. In the context of party competition, a winning coalition is required for one discourse to predominate. However, I also argued that both ideas...

  20. Your Golden Jubilee Party!

    CERN Multimedia

    2004-01-01

    All staff are invited to the Golden Jubilee Staff Party on the evening of 17 September. To mark CERN's 50th anniversary, the Director General would like to invite all staff working at CERN to celebrate the success of the organization. The party will be held on the terrace of the Main Building on 17 September at 4:00 pm. The event will include an address by the DG, music, film clips, drinks and snacks. Your effort, hard work and enthusiasm have been the key to CERN's success. The first of the main events celebrating the organization's Golden Jubilee is for you, so come along and celebrate!

  1. Partying as Everyday Life

    DEFF Research Database (Denmark)

    Demant, Jakob; Østergaard, Jeanette

    2007-01-01

    situates the event in everyday life. By drawing on Maffesoli's (1996) concept of ‘sociality' and Lincoln's (2005) concept of zoning the spatial and social logic of the house, partying is analysed using both qualitative and quantitative material. The analysis suggests that the consumption of alcohol (i...... to reaffirm friendship and is therefore an integrated part of adolescents' everyday life.  ...

  2. Persecution by third parties

    NARCIS (Netherlands)

    Vermeulen, Ben; Spijkerboer, Thomas; Zwaan, Karin; Fernhout, Roel

    1998-01-01

    As a large number of the present day asylum claims concern situations of civil war or of a break down of (central) governmental authority the issue of persecution by third parties is of great importance. Although the main characteristics of the different positions are clear, precise information abou

  3. A cocktail of three virulent bacteriophages prevents Vibrio cholerae infection in animal models

    Science.gov (United States)

    Yen, Minmin; Cairns, Lynne S.; Camilli, Andrew

    2017-01-01

    Effective prevention strategies will be essential in reducing disease burden due to bacterial infections. Here we harness the specificity and rapid-acting properties of bacteriophages as a potential prophylaxis therapy for cholera, a severely dehydrating disease caused by Vibrio cholerae. To this end, we test a cocktail of three virulent phages in two animal models of cholera pathogenesis (infant mouse and rabbit models). Oral administration of the phages up to 24 h before V. cholerae challenge reduces colonization of the intestinal tract and prevents cholera-like diarrhea. None of the surviving V. cholerae colonies are resistant to all three phages. Genome sequencing and variant analysis of the surviving colonies indicate that resistance to the phages is largely conferred by mutations in genes required for the production of the phage receptors. For acute infections, such as cholera, phage prophylaxis could provide a strategy to limit the impact of bacterial disease on human health. PMID:28146150

  4. Evaluation of new antibiotic cocktails against contaminating bacteria found in allograft tissues.

    Science.gov (United States)

    Serafini, Agnese; Riello, Erika; Trojan, Diletta; Cogliati, Elisa; Palù, Giorgio; Manganelli, Riccardo; Paolin, Adolfo

    2016-12-01

    Contamination of retrieved tissues is a major problem for allograft safety. Consequently, tissue banks have implemented decontamination protocols to eliminate microorganisms from tissues. Despite the widespread adoption of these protocols, few comprehensive studies validating such methods have been published. In this manuscript we compare the bactericidal activity of different antibiotic cocktails at different temperatures against a panel of bacterial species frequently isolated in allograft tissues collected at the Treviso Tissue Bank Foundation, a reference organization of the Veneto Region in Italy that was instituted to select, recover, process, store and distribute human tissues. We were able to identify at least two different formulations capable of killing most of the bacteria during prolonged incubation at 4 °C.

  5. Party rules, party resources and the politics of parliamentary democracies

    DEFF Research Database (Denmark)

    Poguntke, Thomas; Scarrow, Susan; Webb, Paul

    2016-01-01

    This article introduces the first findings of the Political Party Database Project, a major survey of party organizations in parliamentary and semi-presidential democracies. The project’s first round of data covers 122 parties in 19 countries. In this article, we describe the scope of the database...

  6. Your Golden Jubilee Party!

    CERN Document Server

    2004-01-01

    All staff are invited to the Golden Jubilee Staff Party on the evening of 17 September To mark CERN's 50th anniversary, the Director General would like to invite all staff working at CERN to celebrate the success of the organization. The party will be held on the terrace of the Main Building on 17 September at 4:00 pm. The event will include an address by the DG, music, film clips, drinks and snacks. Your effort, hard work and enthusiasm have been the key to CERN's success. The first of the main events celebrating the organization's Golden Jubilee is for you, so come along and celebrate! http://www.cern.ch/cern50/

  7. Your Golden Jubilee Party!

    CERN Document Server

    2004-01-01

    All staff are invited to the Golden Jubilee Staff Party on the evening of 17 September. To mark CERN's 50th anniversary, the Director General would like to invite all staff working at CERN to celebrate the success of the organization. The party will be held on the terrace of the Main Building on 17 September at 4:00 pm. The event will include an address by the DG, music, film clips, drinks and snacks! Your effort, hard work and enthusiasm have been the key to CERN's success. The first of the main events celebrating the organization's Golden Jubilee is for you, so come along and celebrate! http://www.cern.ch/cern50/

  8. Fragment-based cocktail crystallography by the Medical Structural Genomics of Pathogenic Protozoa Consortium

    Science.gov (United States)

    Verlinde, Christophe L.M.J.; Fan, Erkang; Shibata, Sayaka; Zhang, Zongsheng; Sun, Zhihua; Deng, Wei; Ross, Jennifer; Kim, Jessica; Xiao, Liren; Arakaki, Tracy L.; Bosch, Jürgen; Caruthers, Jonathan M.; Larson, Eric T.; LeTrong, Isolde; Napuli, Alberto; Kelly, Angela; Mueller, Natasha; Zucker, Frank; Van Voorhis, Wesley C.; Buckner, Frederick S.; Merritt, Ethan A.; Hol, Wim G.J.

    2010-01-01

    The history of fragment-based drug discovery, with an emphasis on crystallographic methods, is sketched, illuminating various contributions, including our own, which preceded the industrial development of the method. Subsequently, the creation of the BMSC fragment cocktails library is described. The BMSC collection currently comprises 68 cocktails of 10 compounds that are shape-wise diverse. The utility of these cocktails for initiating lead discovery in structure-based drug design has been explored by soaking numerous protein crystals obtained by our MSGPP (Medical Structural Genomics of Pathogenic Protozoa) consortium. Details of the fragment selection and cocktail design procedures, as well as examples of the successes obtained are given. The BMSC Fragment Cocktail recipes are available free of charge and are in use in over 20 academic labs. PMID:19929835

  9. Your Golden Jubilee Party!

    CERN Multimedia

    2004-01-01

    http://www.cern.ch/cern50/All staff are invited to the Golden Jubilee Staff Party on the evening of 17 September. To mark CERN's 50th anniversary, the Director-General would like to invite all the people working at CERN to celebrate the success of the organization. The party will be held in the cafeteria and on the terrace (if the weather permits) of the Main Building on 17 September at 4:00 pm. The event will include an address by the DG, music, film clips, drinks, snacks and a surprise music show by the CERN Big Bang Orchestra! Your effort, hard work and enthusiasm have been the key to CERN's success. The first of the main events celebrating the organization's Golden Jubilee is for you, so come along and celebrate! Early closure of Restaurant No. 1 (Bldg. 501, Meyrin site): Friday 17 September at 2:00 p.m. On Friday, 17 September, Restaurant No. 1 (NOVAE) will close at 2:00 p.m. owing to the Golden Jubilee party for the CERN staff. No dinner will be served that evening.

  10. Multi-Party Quantum Steganography

    Science.gov (United States)

    Mihara, Takashi

    2017-02-01

    Steganography has been proposed as a data hiding technique. As a derivation, quantum steganography based on quantum physics has also been proposed. In this paper, we extend the results in presented (Mihara, Phys. Lett. 379, 952 2015) and propose a multi-party quantum steganography technique that combines quantum error-correcting codes with entanglement. The proposed protocol shares an entangled state among n +1 parties and sends n secret messages, corresponding to the n parties, to the other party. With no knowledge of the other secret messages, the n parties can construct a stego message by cooperating with each other. Finally, we propose a protocol for sending qubits using the same technique.

  11. Departies: conceptualizing extended youth parties

    DEFF Research Database (Denmark)

    Fjær, Eivind Grip; Tutenges, Sébastien

    2017-01-01

    Every year, millions of young people travel away from home to party for days or weeks on end in permissive environments, such as music festivals, dance parties, and nightlife resorts. The studies that have been conducted on these extended youth parties have focused primarily on specific risk...... immoral; (4) stylistically, by altering their stylistic expressions through dress, demeanor, and consumption; and (5) experientially, because the parties generate mood and mind alterations. These are overlapping and intertwined elements, the combination of which amounts to a distinct type of youth party...

  12. Departies: conceptualizing extended youth parties

    DEFF Research Database (Denmark)

    Eivind Grip, Fjær,; Tutenges, Sébastien

    2016-01-01

    Every year, millions of young people travel away from home to party for days or weeks on end in permissive environments, such as music festivals, dance parties, and nightlife resorts. The studies that have been conducted on these extended youth parties have focused primarily on specific risk...... immoral; (4) stylistically, by altering their stylistic expressions through dress, demeanor, and consumption; and (5) experientially, because the parties generate mood and mind alterations. These are overlapping and intertwined elements, the combination of which amounts to a distinct type of youth party...

  13. Recent advances of cocktail chemotherapy by combination drug delivery systems.

    Science.gov (United States)

    Hu, Quanyin; Sun, Wujin; Wang, Chao; Gu, Zhen

    2016-03-01

    Combination chemotherapy is widely exploited for enhanced cancer treatment in the clinic. However, the traditional cocktail administration of combination regimens often suffers from varying pharmacokinetics among different drugs. The emergence of nanotechnology offers an unparalleled opportunity for developing advanced combination drug delivery strategies with the ability to encapsulate various drugs simultaneously and unify the pharmacokinetics of each drug. This review surveys the most recent advances in combination delivery of multiple small molecule chemotherapeutics using nanocarriers. The mechanisms underlying combination chemotherapy, including the synergistic, additive and potentiation effects, are also discussed with typical examples. We further highlight the sequential and site-specific co-delivery strategies, which provide new guidelines for development of programmable combination drug delivery systems. Clinical outlook and challenges are also discussed in the end.

  14. Toxic cocktail: methanol poisoning in a tourist to Indonesia.

    Science.gov (United States)

    Gee, Paul; Martin, Elizabeth

    2012-08-01

    Methanol poisoning has become very uncommon in Australasia with regulations that have reduced its retail availability. This report describes a young tourist who developed sudden onset visual failure and rapid breathing 2 days after ingestion of a complimentary local drink called Arrack when travelling in Indonesia. She presented to a hospital in New Zealand with severe metabolic acidosis and a highly toxic methanol level at 17 mmol/L. The cocktail was consumed at a popular tourist bar but is likely to have been mixed from home-brewed or 'moonshine' alcohol tainted with methanol. The aetiology of methanol poisoning, its optic toxicology and therapeutic measures are discussed, as is the concerning number of methanol poisoning cases among tourists to Indonesia over recent years.

  15. Cocoa fermentations conducted with a defined microbial cocktail inoculum.

    Science.gov (United States)

    Schwan, R F

    1998-04-01

    Cocoa fermentations were performed in wooden boxes under the following four experimental regimens: beans naturally fermented with wild microflora; aseptically prepared beans with no inoculum; and beans inoculated with a defined cocktail containing microorganisms at a suitable concentration either at zero time or by using phased additions at appropriate times. The cocktail used consisted of a yeast, Saccharomyces cerevisiae var. chevalieri, two lactic acid bacterial species, Lactobacillus lactis and Lactobacillus plantarum, and two acetic acid bacterial species, Acetobacter aceti and Gluconobacter oxydans subsp. suboxydans. The parameters measured were cell counts (for yeasts, filamentous fungi, lactic acid bacteria, acetic acid bacteria, and spore formers, including reisolation and identification of all residual cell types), sugar, ethanol, acetic acid, and lactic acid contents (and contents of other organic acids), pH, and temperature. A cut test for bean quality and a sensorial analysis of chocolate made from the beans were also performed. The natural fermentation mimicked exactly the conditions in 800-kg boxes on farms. The aseptic box remained largely free of microflora throughout the study, and no significant biochemical changes occurred. With the zero-time inoculum the fermentation was almost identical to the natural fermentation. The fermentation with the phased-addition inoculum was similar, but many changes in parameters were slower and less pronounced, which led to a slightly poorer end product. The data show that the nearly 50 common species of microorganisms found in natural fermentations can be replaced by a judicious selection and concentration of members of each physiological group. This is the first report of successful use of a defined, mixed starter culture in such a complex fermentation, and it should lead to chocolate of more reliable and better quality.

  16. Does the mosquito have more of a role in certain cancers than is currently appreciated? - The mosquito cocktail hypothesis.

    Science.gov (United States)

    Ward, M; Ward, A; Johansson, O

    2016-01-01

    The International Agency for Research on Cancer recognises five viruses, one bacterium and three parasites as having a causal relationship to cancer, and one virus, HIV, that by suppressing the immune system assists in the development of cancer. In addition numerous researchers have claimed links between cancer and other viruses and bacteria, many of which have been isolated from tumours. Excluding the non-causal HIV, 33 infectious agents linked with cancer have been identified in the literature, 27 of which have one thing in common: they are all present in mosquitoes. There are over 3000 species of mosquito and very few have been fully analysed, so the six remaining infectious agents may be present in some unexamined species. This hypothesis proposes that more cancers than are presently appreciated may arise from the long-term outcome of a mosquito bite, which by releasing a complex cocktail of up to 60 infectious agents directly into the blood stream, often results in contemporaneous immuno-suppression and a multiplicity of co-infections. These co-infections may act synergistically in whole, or in part, and in complex ways. Whether and if so which type of cancer ensues will depend on the constituent ingredients in the cocktail, determined by multiple factors such as the mosquito's drinking and feeding patterns, number of previous blood-meals and the variety of intermediate hosts from which these meals are taken. Only a few mosquito species carry malaria, dengue fever, chikungunya and the other recognised serious human ailments. This hypothesis suggests that the number of species carrying the cancer cocktail will be few in number but collectively have a worldwide presence. Copyright © 2015 Elsevier Ltd. All rights reserved.

  17. Inhibition of melanoma growth by subcutaneous administration of hTERTC27 viral cocktail in C57BL/6 mice.

    Directory of Open Access Journals (Sweden)

    Longfei Huo

    Full Text Available BACKGROUND: hTERTC27 is a 27 kDa C-terminal polypeptide of human telomerase reverse transcriptase that has previously been shown to reduce tumorigenicity of HeLa cells and suppress growth of xenografted glioblastoma in nude mice. Although ectopic expression of hTERTC27 upregulated genes that are involved in apoptosis, cell cycle, and immune response, the mechanism for hTERTC27-induced tumor suppression has not been completely elucidated. Since hTERT was identified as a universal tumor-associated antigen, we hypothesize that hTERTC27 inhibits tumor growth in vivo through activation of anti-tumor immune response. METHODOLOGY/PRINCIPAL FINDING: Immunocompetent C57BL/6 mice were used for mouse B16 melanoma model. Mice bearing B16 melanoma were administered rAAV-/rAdv viral cocktail expressing hTERTC27, and tumor growth was monitored after viral cocktail treatment. Blood and splenocytes were used to determine the level of cytokines and the activity of immune cells, respectively. B16 tumor growth was significantly inhibited by subcutaneous administration of a single dose of 1.5×10(11 vg rAAV-hTERTC27 and 2.5×10(9 pfu rAdv-hTERTC27 viral cocktail (rAAV-/rAdv-hTERTC27. The population and cytotoxicity of NK cells in the mice were significantly augmented by rAAV-/rAdv-hTERTC27 treatment, and selective depletion of the NK cell population in mice by intraperitoneal injection of anti-GM1 antibody abrogated the growth suppression of melanoma induced by rAAV-/rAdv-hTERTC27 administration. CONCLUSION: Activation of NK cells by administration of rAAV-/rAdv-hTERTC27 is critical for growth suppression of melanoma in mouse model.

  18. The study of vial and cocktail for tritium radioactivity analysis of rain water by liquid scintillation counter

    Energy Technology Data Exchange (ETDEWEB)

    Noh, Sung Jin; Kim, Hyo Jin; Kim, Hyun; Lee, Man Woo; Jeong, Dong Hyeok; Yang, Kwang Mo; Kang, Yeong Rok [Research Center, Dongnam Institute of Radiological and Medical Sciences, Busan (Korea, Republic of); Nam, Sang Hee [Dept. of Biomedical Engineering, Inje University, Gimhae (Korea, Republic of)

    2015-10-15

    Even though the current method for tritium (3H) analysis is routine, for the case of the low level of tritium in the environment, special conditions have to be fulfilled in order to obtain accurate and reliable tritium measurements. There are very little comparative data concerning commercial scintillating cocktails. The best cocktails for measuring tritium are those based on benzene derived solvent, and the worse cocktails are those which have complex chemical composition or contain too small concentration of scintillators. The aim of study was to investigate various vials and cocktails by comparison with the combination of few different scintillation cocktails and vials in our routine measurements according to count, efficiency, and the figure of merit (FOM). The comparison of three types of vials with scintillation cocktails for tritium activity analysis of rain water shows that glass vials have higher count rates and HiSafe 3 cocktails have lower FOM.

  19. Suppressing tumor growth of nasopharyngeal carcinoma by hTERTC27 polypeptide delivered through adeno-associated virus plus adenovirus vector cocktail

    Institute of Scientific and Technical Information of China (English)

    Xiong Liu; Xiang-Ping Li; Ying Peng; Samuel S.Ng; Hong Yao; Zi-Feng Wang; Xiao-Mei Wang; Hsiang-Fu Kung; Marie C.M.Lin

    2012-01-01

    Nasopharyngeal carcinoma(NPC) is a metastatic carcinoma that is highly prevalent in Southeast Asia.Our laboratory has previously demonstrated that the C-terminal 27-kDa polypeptide of human telomerase reverse transcriptase (hTERTC27) inhibits the growth and tumorigenicity of human glioblastoma and melanoma cells.In this study,we investigated the antitumor effect of hTERTC27 in human C666-1 NPC cells xenografted in a nude mouse model.A cocktail of vectors comprising recombinant adeno-associated virus (rAAV) and recombinant adenovirus (rAdv) that each carry hTERTC27 (rAAV-hTERTC27 and rAdv-hTERTC27; the cocktail was abbreviated to rAAV/rAdv-hTERTC27) was more effective than either rAAV-hTERTC27 or rAdv-hTERTC27 alone in inhibiting the growth of C666-1 NPC xenografts.Furthermore,we established three tumors on each mouse and injected rAAV/rAdv-hTERTC27 into one tumor per mouse.Although hTERTC27 expression could only be detected in the injected tumors,reduced tumor growth was observed in the injected tumor as well as the uninjected tumors,demonstrating that the vector cocktail could provoke an antitumor effect on distant,metastasized tumors.Further studies showed the observed antitumor effects included inducing necrosis and apoptosis and reducing microvessel density.Together,our data suggest that the rAAV/rAdv-hTERTC27 cocktail can potently inhibit NPC tumor growth in both local and metastasized tumors and should be further developed as a novel gene therapy strategy for NPC.

  20. Enhanced antimicrobial activity of peptide‐cocktails against common bacterial contaminants of ex vivo stored platelets

    National Research Council Canada - National Science Library

    Mohan, K. V. K; Rao, S. Sainath; Gao, Y; Atreya, C. D; Grobusch, M

    2014-01-01

    .... In the present study, we selected PD 3, PD 4, RW 2, RW 3 and RW 4 and evaluated each individual peptide and their various combinations to see whether the cocktail regimen enhances the antimicrobial...

  1. A cytochrome P450 phenotyping cocktail causing unexpected adverse reactions in female volunteers

    DEFF Research Database (Denmark)

    Pedersen, Rasmus Steen; Damkier, Per; Hougaard Christensen, Mette Marie

    2013-01-01

    A four-drug cytochrome P450 (CYP) phenotyping cocktail was developed to rapidly and safely determine CYP2D6, CYP2C19, CYP2C9 and CYP1A2 enzyme activity and phenotype.......A four-drug cytochrome P450 (CYP) phenotyping cocktail was developed to rapidly and safely determine CYP2D6, CYP2C19, CYP2C9 and CYP1A2 enzyme activity and phenotype....

  2. What Can We Learn from a Metagenomic Analysis of a Georgian Bacteriophage Cocktail?

    Directory of Open Access Journals (Sweden)

    Henrike Zschach

    2015-12-01

    Full Text Available Phage therapy, a practice widespread in Eastern Europe, has untapped potential in the combat against antibiotic-resistant bacterial infections. However, technology transfer to Western medicine is proving challenging. Bioinformatics analysis could help to facilitate this endeavor. In the present study, the Intesti phage cocktail, a key commercial product of the Eliava Institute, Georgia, has been tested on a selection of bacterial strains, sequenced as a metagenomic sample, de novo assembled and analyzed by bioinformatics methods. Furthermore, eight bacterial host strains were infected with the cocktail and the resulting lysates sequenced and compared to the unamplified cocktail. The analysis identified 23 major phage clusters in different abundances in the cocktail, among those clusters related to the ICTV genera T4likevirus, T5likevirus, T7likevirus, Chilikevirus and Twortlikevirus, as well as a cluster that was quite distant to the database sequences and a novel Proteus phage cluster. Examination of the depth of coverage showed the clusters to have different abundances within the cocktail. The cocktail was found to be composed primarily of Myoviridae (35% and Siphoviridae (32%, with Podoviridae being a minority (15%. No undesirable genes were found.

  3. Between Party, Parents and Peers

    DEFF Research Database (Denmark)

    Bregnbæk, Susanne

    2012-01-01

    This article explores the lived contradictions entailed in being a young member of the Chinese Communist Party (ccp) today. The focus is on how political and existential issues intersect. It explores party membership as a strategy for personal mobility among Beijing elite university students...

  4. End of Year Party Time

    Institute of Scientific and Technical Information of China (English)

    GREGGWYCHERLEY

    2004-01-01

    We all remember the raucous end of year office party, where everybody drinks too much, wears silly party hats and ends up doing something best forgotten.Traditionally the venue is the office itself,strung up with a few makeshift decorations,combined with a table-load of food and drink, and offyou go.

  5. Injections, cocktails and diviners: therapeutic flexibility in the context of malaria elimination and drug resistance in Northeast Cambodia.

    Directory of Open Access Journals (Sweden)

    Charlotte Gryseels

    Full Text Available BACKGROUND: Adherence to effective malaria medication is extremely important in the context of Cambodia's elimination targets and drug resistance containment. Although the public sector health facilities are accessible to the local ethnic minorities of Ratanakiri province (Northeast Cambodia, their illness itineraries often lead them to private pharmacies selling "cocktails" and artemether injections, or to local diviners prescribing animal sacrifices to appease the spirits. METHODS: The research design consisted of a mixed methods study, combining qualitative (in-depth interviews and participant observation and quantitative methods (household and cross-sectional survey. RESULTS: Three broad options for malaria treatment were identified: i the public sector; ii the private sector; iii traditional treatment based on divination and ceremonial sacrifice. Treatment choice was influenced by the availability of treatment and provider, perceived side effects and efficacy of treatments, perceived etiology of symptoms, and patient-health provider encounters. Moreover, treatment paths proved to be highly flexible, changing mostly in relation to the perceived efficacy of a chosen treatment. CONCLUSIONS: Despite good availability of anti-malarial treatment in the public health sector, attendance remained low due to both structural and human behavioral factors. The common use and under-dosage of anti-malaria monotherapy in the private sector (single-dose injections, single-day drug cocktails represents a threat not only for individual case management, but also for the regional plan of drug resistance containment and malaria elimination.

  6. 16 CFR 1507.11 - Party poppers.

    Science.gov (United States)

    2010-01-01

    ... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Party poppers. 1507.11 Section 1507.11... FIREWORKS DEVICES § 1507.11 Party poppers. Party poppers (also known by other names such as “Champagne Party Poppers,” and “Party Surprise Poppers,”) shall not contain more than 0.25 grains of...

  7. Novel microRNA prosurvival cocktail for improving engraftment and function of cardiac progenitor cell transplantation.

    Science.gov (United States)

    Hu, Shijun; Huang, Mei; Nguyen, Patricia K; Gong, Yongquan; Li, Zongjin; Jia, Fangjun; Lan, Feng; Liu, Junwei; Nag, Divya; Robbins, Robert C; Wu, Joseph C

    2011-09-13

    Although stem cell therapy has provided a promising treatment for myocardial infarction, the low survival of the transplanted cells in the infarcted myocardium is possibly a primary reason for failure of long-term improvement. Therefore, the development of novel prosurvival strategies to boost stem cell survival will be of significant benefit to this field. Cardiac progenitor cells (CPCs) were isolated from transgenic mice, which constitutively express firefly luciferase and green fluorescent protein. The CPCs were transduced with individual lentivirus carrying the precursor of miR-21, miR-24, and miR-221, a cocktail of these 3 microRNA precursors, or green fluorescent protein as a control. After challenge in serum free medium, CPCs treated with the 3 microRNA cocktail showed significantly higher viability compared with untreated CPCs. After intramuscular and intramyocardial injections, in vivo bioluminescence imaging showed that microRNA cocktail-treated CPCs survived significantly longer after transplantation. After left anterior descending artery ligation, microRNA cocktail-treated CPCs boost the therapeutic efficacy in terms of functional recovery. Histological analysis confirmed increased myocardial wall thickness and CPC engraftment in the myocardium with the microRNA cocktail. Finally, we used bioinformatics analysis and experimental validation assays to show that Bim, a critical apoptotic activator, is an important target gene of the microRNA cocktail, which collectively can bind to the 3'UTR region of Bim and suppress its expression. We have demonstrated that a microRNA prosurvival cocktail (miR-21, miR-24, and miR-221) can improve the engraftment of transplanted cardiac progenitor cells and therapeutic efficacy for treatment of ischemic heart disease.

  8. Cocktail sedation containing propofol versus conventional sedation for ERCP: a prospective, randomized controlled study

    Directory of Open Access Journals (Sweden)

    Angsuwatcharakon Phonthep

    2012-08-01

    Full Text Available Abstract Background ERCP practically requires moderate to deep sedation controlled by a combination of benzodiazepine and opiod. Propofol as a sole agent may cause oversedation. A combination (cocktail of infused propofol, meperidine, and midazolam can reduce the dosage of propofol and we hypothesized that it might decrease the risk of oversedation. We prospectively compare the efficacy, recovery time, patient satisfactory, and side effects between cocktail and conventional sedations in patients undergoing ERCP. Methods ERCP patients were randomized into 2 groups; the cocktail group (n = 103 and the controls (n = 102. For induction, a combination of 25 mg of meperidine and 2.5 mg of midazolam were administered in both groups. In the cocktail group, a bolus dose of propofol 1 mg/kg was administered and continuously infused. In the controls, 25 mg of meperidine or 2.5 mg/kg of midazolam were titrated to maintain the level of sedation. Results In the cocktail group, the average administration rate of propofol was 6.2 mg/kg/hr. In the control group; average weight base dosage of meperidine and midazolam were 1.03 mg/kg and 0.12 mg/kg, respectively. Recovery times and patients’ satisfaction scores in the cocktail and control groups were 9.67 minutes and 12.89 minutes (P = 0.045, 93.1and 87.6 (P P Conclusions Cocktail sedation containing propofol provides faster recovery time and better patients’ satisfaction for patients undergoing ERCP. However, mild degree of desaturation may still develop. Trial registration ClinicalTrials.gov, NCT01540084

  9. Effects of polar bear and killer whale derived contaminant cocktails on marine mammal immunity.

    Science.gov (United States)

    Desforges, Jean-Pierre; Levin, Milton; Jasperse, Lindsay; De Guise, Sylvain; Eulaers, Igor; Letcher, Robert J; Acquarone, Mario; Nordoy, Erling; Folkow, Lars; Hammer Jensen, Trine; Grondahl, Carsten; Bertelsen, Mads F; St Leger, Judy; Almunia, Javier; Sonne, Christian; Dietz, Rune

    2017-09-06

    Most controlled toxicity studies use single chemical exposures that do not represent the real world situation of complex mixtures of known and unknown natural and anthropogenic substances. In the present study, complex contaminant cocktails derived from the blubber of polar bears (PB; Ursus maritimus) and killer whales (KW; Orcinus orca) were used for in vitro concentration-response experiments with PB, cetacean and seal spp. immune cells to evaluate the effect of realistic contaminant mixtures on various immune functions. Cytotoxic effects of the PB cocktail occurred at lower concentrations than the KW cocktail (1 vs 16 µg/ml), likely due to differences in contaminant profiles in the mixtures derived from the adipose of each species. Similarly, significant reduction of lymphocyte proliferation occured at much lower exposures in the PB cocktail (EC50: 0.94 vs 6.06 µg/ml; P < 0.01), while the KW cocktail caused a much faster decline in proliferation (slope: 2.9 vs 1.7; P = 0.04). Only the KW cocktail modulated natural killer (NK) cell activity and neutrophil and monocyte phagocytosis in a concentration- and species-dependent manner. No clear sensitivity differences emerged when comparing cetaceans, seals and PB. Our results showing lower effect levels for complex mixtures relative to single compounds suggest that previous risk assessments underestimate the effects of real world contaminant exposure on immunity. Our results using blubber-derived contaminant cocktails add realism to in vitro exposure experiments and confirm the immunotoxic risk marine mammals face from exposure to complex mixtures of environmental contaminants.

  10. The Basic Points of the Accomplishment to the Communists---The Spirit of the Chinese Communist Party;the Human Nature;the Personality%共产党员党性修养的基点--党性·人性·个性

    Institute of Scientific and Technical Information of China (English)

    孙秀民

    2014-01-01

    The accomplishments to the Communists should adhere to the spirit of the Chinese Communist Party firstly. But sticking to the spirit of the Chinese Communist Party should be people-oriented, respect humanity and fulfill the personality of the Communists. Adhering to the spirit of the Chinese Communist Party is a core and root; respecting for humanity is a premise and foundation; fulfilling the personality of the Communists is the main behavior of respecting the personality, and it also mainly displays the spirit of the Chinese Communist Party. The spirit of the Chinese Communist Party, the human nature and the personality are the main behaviors of the accomplishments to the Communists.%共产党员的党性修养首先要坚持党性,但坚持党性要以人为本,尊重人性、发挥党员个性。坚持党性是核心,是根本;尊重人性是基础,是前提;发挥党员的个性是尊重人性的体现,也是党性的具体展现。党性、人性、个性是中共党员党性修养的基点。

  11. REFLECTIONS ON WAGAR ' S WORLD PARTY

    Directory of Open Access Journals (Sweden)

    Albert Bergesen

    2015-08-01

    Full Text Available Our task is to reflect upon Wagar's idea of a world party. In case such reflections are affected by the recent historical situation of the collapse of communism/existing socialism in 1989 and the implications this has for visions of progressive politics going into the 21st century. This event colors most political thinking, although for many the response has been that existing socialism was not real socialism, or that existing socialism was but the Stalinist deformation that, if avoided in the future, the 1917 project could again be resumed and human history and social relations remade anew. I don't see it that way. What existing socialism stood for in terms of the role of a vanguard party taking state power for the larger good is, now after the fall, I think off the board as a realistic program that can be sold to anyone.

  12. Evaluation of Cocktails with Recombinant Proteins of Mycobacterium bovis for a Specific Diagnosis of Bovine Tuberculosis

    Directory of Open Access Journals (Sweden)

    María Laura Mon

    2014-01-01

    Full Text Available The Delayed type hypersensitivity skin test (DTH and interferon-gamma assay are used for the diagnosis of bovine tuberculosis (TBB. The specificity of these diagnoses, however, is compromised because both are based on the response against purified protein derivative of Mycobacterium bovis (PPD-B. In this study, we assessed the potential of two cocktails containing M. bovis recombinant proteins: cocktail 1 (C1: ESAT-6, CFP-10 and MPB83 and cocktail 2 (C2: ESAT-6, CFP-10, MPB83, HspX, TB10.3, and MPB70. C1, C2, and PPD-B showed similar response by DTH in M. bovis-sensitized guinea pigs. Importantly, C1 induced a lower response than PPD-B in M. avium-sensitized guinea pigs. In cattle, C1 displayed better performance than PPD-B and C2; indeed, C1 showed the least detection of animals either vaccinated or Map-infected. To optimize the composition of the cocktails, we obtained protein fractions from PPD-B and tested their immunogenicity in experimentally M. bovis-infected cattle. In one highly reactive fraction, seven proteins were identified. The inclusion of FixB in C1 enhanced the recognition of M. bovis-infected cattle without compromising specificity. Our data provide a promising basis for the future development of a cocktail for TBB detection without interference by the presence of sensitized or infected animals with other mycobacteria.

  13. Evaluation of Cocktails with Recombinant Proteins of Mycobacterium bovis for a Specific Diagnosis of Bovine Tuberculosis

    Science.gov (United States)

    Mon, María Laura; Moyano, Roberto Damián; Viale, Mariana Noelia; Colombatti Olivieri, María Alejandra; Gamietea, Ignacio José; Montenegro, Valeria Noely; Alonso, Bernardo; Santangelo, María de la Paz; Singh, Mahavir; Duran, Rosario; Romano, María Isabel

    2014-01-01

    The Delayed type hypersensitivity skin test (DTH) and interferon-gamma assay are used for the diagnosis of bovine tuberculosis (TBB). The specificity of these diagnoses, however, is compromised because both are based on the response against purified protein derivative of Mycobacterium bovis (PPD-B). In this study, we assessed the potential of two cocktails containing M. bovis recombinant proteins: cocktail 1 (C1): ESAT-6, CFP-10 and MPB83 and cocktail 2 (C2): ESAT-6, CFP-10, MPB83, HspX, TB10.3, and MPB70. C1, C2, and PPD-B showed similar response by DTH in M. bovis-sensitized guinea pigs. Importantly, C1 induced a lower response than PPD-B in M. avium-sensitized guinea pigs. In cattle, C1 displayed better performance than PPD-B and C2; indeed, C1 showed the least detection of animals either vaccinated or Map-infected. To optimize the composition of the cocktails, we obtained protein fractions from PPD-B and tested their immunogenicity in experimentally M. bovis-infected cattle. In one highly reactive fraction, seven proteins were identified. The inclusion of FixB in C1 enhanced the recognition of M. bovis-infected cattle without compromising specificity. Our data provide a promising basis for the future development of a cocktail for TBB detection without interference by the presence of sensitized or infected animals with other mycobacteria. PMID:25110654

  14. Monosaccharides and Ethanol Production from Superfine Ground Sugarcane Bagasse Using Enzyme Cocktail

    Directory of Open Access Journals (Sweden)

    Jingbo Li

    2014-03-01

    Full Text Available In this work, the effect of particle size on the enzymatic hydrolysis of milled and sieved sugarcane bagasse (SCB was studied. The enzymatic hydrolysis and fermentability of superfine ground SCB (SGP400 using an enzyme cocktail strategy were also explored. Particle size reduction improved the enzymatic hydrolysis. The highest glucose yield was 44.75%, which was obtained from SGP400. The enzyme cocktail strategy greatly enhanced the glucose and xylose yield. The maximum glucose and xylose yield was from the enzyme cocktail of cellulase, xylanase, and pectinase. Synergistic action between xylanase and pectinase as well as cellulase and pectinase was quite noticeable. Hydrolysis times affected the degree of synergism. Ethanol production was carried out by employing simultaneous saccharification and fermentation (SSF and semi-SSF using enzymes and their cocktails. Semi-SSF was found to be the better one compared with SSF. Xylanase and pectinase aided the ethanol production in both fermentation modes. Ethanol yield was 7.81 and 7.30 g/L for semi-SSF and SSF, respectively by using an enzyme cocktail of cellulase, β-glucosidase, pectinase, and xylanase.

  15. A thermophilic ionic liquid-tolerant cellulase cocktail for the production of cellulosic biofuels.

    Directory of Open Access Journals (Sweden)

    Joshua I Park

    Full Text Available Generation of biofuels from sugars in lignocellulosic biomass is a promising alternative to liquid fossil fuels, but efficient and inexpensive bioprocessing configurations must be developed to make this technology commercially viable. One of the major barriers to commercialization is the recalcitrance of plant cell wall polysaccharides to enzymatic hydrolysis. Biomass pretreatment with ionic liquids (ILs enables efficient saccharification of biomass, but residual ILs inhibit both saccharification and microbial fuel production, requiring extensive washing after IL pretreatment. Pretreatment itself can also produce biomass-derived inhibitory compounds that reduce microbial fuel production. Therefore, there are multiple points in the process from biomass to biofuel production that must be interrogated and optimized to maximize fuel production. Here, we report the development of an IL-tolerant cellulase cocktail by combining thermophilic bacterial glycoside hydrolases produced by a mixed consortia with recombinant glycoside hydrolases. This enzymatic cocktail saccharifies IL-pretreated biomass at higher temperatures and in the presence of much higher IL concentrations than commercial fungal cocktails. Sugars obtained from saccharification of IL-pretreated switchgrass using this cocktail can be converted into biodiesel (fatty acid ethyl-esters or FAEEs by a metabolically engineered strain of E. coli. During these studies, we found that this biodiesel-producing E. coli strain was sensitive to ILs and inhibitors released by saccharification. This cocktail will enable the development of novel biomass to biofuel bioprocessing configurations that may overcome some of the barriers to production of inexpensive cellulosic biofuels.

  16. Efficiency Enhancement of Cocktail Dye of Ixora coccinea and Tradescantia spathacea in DSSC.

    Science.gov (United States)

    Zolkepli, Zularif; Lim, Andery; Ekanayake, Piyasiri; Tennakoon, Kushan

    2015-01-01

    The use of anthocyanin dyes extracted from epidermal leaves of Tradescantia spathacea (Trant) and petals of Ixora coccinea (IX) was evaluated in the application of dye-sensitized solar cells (DSSCs). Subsequently, cocktail anthocyanin dyes from these dyes were prepared and how they enhanced the cell's overall performance was assessed using five different volume-to-volume ratios. Cocktail dyes absorbed a wider range of light in the visible region, thus increasing the cell efficiencies of the cocktail dyes when compared to the DSSC sensitized by individual dyes. The surface charge (zeta-potential), average size of aggregated anthocyanin molecules (zetasizer), and anthocyanin stability in different storage temperatures were analyzed and recorded. Lower size of aggregated dye molecules as revealed from the cocktail dyes ensured better adsorption onto the TiO2 film. Tradescantia/Ixora pigments mixed in 1 : 4 ratio showed the highest cell efficiency of η = 0.80%, under the irradiance of 100 mW cm(-2), with a short-circuit current density 4.185 mA/cm(2), open-circuit voltage of 0.346 V, and fill factor of 0.499. It was found that the desired storage temperature for these cocktail dyes to be stable over time was -20°C, in which the anthocyanin half-life was about approximately 1727 days.

  17. Efficiency Enhancement of Cocktail Dye of Ixora coccinea and Tradescantia spathacea in DSSC

    Directory of Open Access Journals (Sweden)

    Zularif Zolkepli

    2015-01-01

    Full Text Available The use of anthocyanin dyes extracted from epidermal leaves of Tradescantia spathacea (Trant and petals of Ixora coccinea (IX was evaluated in the application of dye-sensitized solar cells (DSSCs. Subsequently, cocktail anthocyanin dyes from these dyes were prepared and how they enhanced the cell’s overall performance was assessed using five different volume-to-volume ratios. Cocktail dyes absorbed a wider range of light in the visible region, thus increasing the cell efficiencies of the cocktail dyes when compared to the DSSC sensitized by individual dyes. The surface charge (zeta-potential, average size of aggregated anthocyanin molecules (zetasizer, and anthocyanin stability in different storage temperatures were analyzed and recorded. Lower size of aggregated dye molecules as revealed from the cocktail dyes ensured better adsorption onto the TiO2 film. Tradescantia/Ixora pigments mixed in 1 : 4 ratio showed the highest cell efficiency of η=0.80%, under the irradiance of 100 mW cm−2, with a short-circuit current density 4.185 mA/cm2, open-circuit voltage of 0.346 V, and fill factor of 0.499. It was found that the desired storage temperature for these cocktail dyes to be stable over time was −20°C, in which the anthocyanin half-life was about approximately 1727 days.

  18. A Thermophilic Ionic Liquid-Tolerant Cellulase Cocktail for the Production of Cellulosic Biofuels

    Science.gov (United States)

    Park, Joshua I.; Steen, Eric J.; Burd, Helcio; Evans, Sophia S.; Redding-Johnson, Alyssa M.; Batth, Tanveer; Benke, Peter I.; D'haeseleer, Patrik; Sun, Ning; Sale, Kenneth L.; Keasling, Jay D.; Lee, Taek Soon; Petzold, Christopher J.; Mukhopadhyay, Aindrila; Singer, Steven W.; Simmons, Blake A.; Gladden, John M.

    2012-01-01

    Generation of biofuels from sugars in lignocellulosic biomass is a promising alternative to liquid fossil fuels, but efficient and inexpensive bioprocessing configurations must be developed to make this technology commercially viable. One of the major barriers to commercialization is the recalcitrance of plant cell wall polysaccharides to enzymatic hydrolysis. Biomass pretreatment with ionic liquids (ILs) enables efficient saccharification of biomass, but residual ILs inhibit both saccharification and microbial fuel production, requiring extensive washing after IL pretreatment. Pretreatment itself can also produce biomass-derived inhibitory compounds that reduce microbial fuel production. Therefore, there are multiple points in the process from biomass to biofuel production that must be interrogated and optimized to maximize fuel production. Here, we report the development of an IL-tolerant cellulase cocktail by combining thermophilic bacterial glycoside hydrolases produced by a mixed consortia with recombinant glycoside hydrolases. This enzymatic cocktail saccharifies IL-pretreated biomass at higher temperatures and in the presence of much higher IL concentrations than commercial fungal cocktails. Sugars obtained from saccharification of IL-pretreated switchgrass using this cocktail can be converted into biodiesel (fatty acid ethyl-esters or FAEEs) by a metabolically engineered strain of E. coli. During these studies, we found that this biodiesel-producing E. coli strain was sensitive to ILs and inhibitors released by saccharification. This cocktail will enable the development of novel biomass to biofuel bioprocessing configurations that may overcome some of the barriers to production of inexpensive cellulosic biofuels. PMID:22649505

  19. A thermophilic ionic liquid-tolerant cellulase cocktail for the production of cellulosic biofuels.

    Science.gov (United States)

    Park, Joshua I; Steen, Eric J; Burd, Helcio; Evans, Sophia S; Redding-Johnson, Alyssa M; Batth, Tanveer; Benke, Peter I; D'haeseleer, Patrik; Sun, Ning; Sale, Kenneth L; Keasling, Jay D; Lee, Taek Soon; Petzold, Christopher J; Mukhopadhyay, Aindrila; Singer, Steven W; Simmons, Blake A; Gladden, John M

    2012-01-01

    Generation of biofuels from sugars in lignocellulosic biomass is a promising alternative to liquid fossil fuels, but efficient and inexpensive bioprocessing configurations must be developed to make this technology commercially viable. One of the major barriers to commercialization is the recalcitrance of plant cell wall polysaccharides to enzymatic hydrolysis. Biomass pretreatment with ionic liquids (ILs) enables efficient saccharification of biomass, but residual ILs inhibit both saccharification and microbial fuel production, requiring extensive washing after IL pretreatment. Pretreatment itself can also produce biomass-derived inhibitory compounds that reduce microbial fuel production. Therefore, there are multiple points in the process from biomass to biofuel production that must be interrogated and optimized to maximize fuel production. Here, we report the development of an IL-tolerant cellulase cocktail by combining thermophilic bacterial glycoside hydrolases produced by a mixed consortia with recombinant glycoside hydrolases. This enzymatic cocktail saccharifies IL-pretreated biomass at higher temperatures and in the presence of much higher IL concentrations than commercial fungal cocktails. Sugars obtained from saccharification of IL-pretreated switchgrass using this cocktail can be converted into biodiesel (fatty acid ethyl-esters or FAEEs) by a metabolically engineered strain of E. coli. During these studies, we found that this biodiesel-producing E. coli strain was sensitive to ILs and inhibitors released by saccharification. This cocktail will enable the development of novel biomass to biofuel bioprocessing configurations that may overcome some of the barriers to production of inexpensive cellulosic biofuels.

  20. Party Newspapers Perspectives and Choices

    Directory of Open Access Journals (Sweden)

    Paula Espírito Santo

    2016-04-01

    Full Text Available The focus of this article is on how different parties use its party newspapers as instruments of political communication, in the current political context, particularly in Portuguese press. As is to be expected, the newspapers reproduce the party’s vision and help disseminate their leaders’ political ideals and proposals. There are, however, significant differences in the history and format of each publication, in terms of the way the political message is put across and the scope of the subjects addressed. This article is based on a content analysis technique, through a categorical qualitative and quantitative systematization. The expected results are centered on the discussion about the communication strategies of ideologically different party newspapers which are visible in front pages. Moreover, this study is expected to contribute to the discussion about personalization in politics, focusing and considering in particular party newspapers. This is a subject that has been scarcely investigated in communication studies and in political science, despite the presence and ideological place of party newspapers in the past and at present. The aim of this article is to make a contribution toward demonstrating and boosting the interest of this important support to party life and politics.

  1. Applying Spatial Audio to Human Interfaces: 25 Years of NASA Experience

    Science.gov (United States)

    Begault, Durand R.; Wenzel, Elizabeth M.; Godfrey, Martine; Miller, Joel D.; Anderson, Mark R.

    2010-01-01

    From the perspective of human factors engineering, the inclusion of spatial audio within a human-machine interface is advantageous from several perspectives. Demonstrated benefits include the ability to monitor multiple streams of speech and non-speech warning tones using a cocktail party advantage, and for aurally-guided visual search. Other potential benefits include the spatial coordination and interaction of multimodal events, and evaluation of new communication technologies and alerting systems using virtual simulation. Many of these technologies were developed at NASA Ames Research Center, beginning in 1985. This paper reviews examples and describes the advantages of spatial sound in NASA-related technologies, including space operations, aeronautics, and search and rescue. The work has involved hardware and software development as well as basic and applied research.

  2. Optimal drug cocktail design: methods for targeting molecular ensembles and insights from theoretical model systems.

    Science.gov (United States)

    Radhakrishnan, Mala L; Tidor, Bruce

    2008-05-01

    Drug resistance is a significant obstacle in the effective treatment of diseases with rapidly mutating targets, such as AIDS, malaria, and certain forms of cancer. Such targets are remarkably efficient at exploring the space of functional mutants and at evolving to evade drug binding while still maintaining their biological role. To overcome this challenge, drug regimens must be active against potential target variants. Such a goal may be accomplished by one drug molecule that recognizes multiple variants or by a drug "cocktail"--a small collection of drug molecules that collectively binds all desired variants. Ideally, one wants the smallest cocktail possible due to the potential for increased toxicity with each additional drug. Therefore, the task of designing a regimen for multiple target variants can be framed as an optimization problem--find the smallest collection of molecules that together "covers" the relevant target variants. In this work, we formulate and apply this optimization framework to theoretical model target ensembles. These results are analyzed to develop an understanding of how the physical properties of a target ensemble relate to the properties of the optimal cocktail. We focus on electrostatic variation within target ensembles, as it is one important mechanism by which drug resistance is achieved. Using integer programming, we systematically designed optimal cocktails to cover model target ensembles. We found that certain drug molecules covered much larger regions of target space than others, a phenomenon explained by theory grounded in continuum electrostatics. Molecules within optimal cocktails were often dissimilar, such that each drug was responsible for binding variants with a certain electrostatic property in common. On average, the number of molecules in the optimal cocktails correlated with the number of variants, the differences in the variants' electrostatic properties at the binding interface, and the level of binding affinity

  3. Nephrotoxicity of recreational party drugs.

    Science.gov (United States)

    Berney-Meyer, Linda; Putt, Tracey; Schollum, John; Walker, Robert

    2012-02-01

    N-benzylpiperazine (BZP) is the active ingredient in recreational 'party' pills with a stimulant, euphoric mechanism of action akin to that of 3,4-methylenedioxymethamphetamine (MDMA or ecstasy). Many people (ab)use BZP-based party pills usually without any significant toxic effects. However, nephrotoxicity secondary to hyperthermia and rhabdomyolysis has been reported. Another serious renal-related side-effect is hyponatraemia with acute cerebral oedema. There is also evidence that these agents may have a specific toxic effect producing acute kidney injury. Thus, acute kidney injury either direct or secondary to the effects of BZP or MDMA need to be considered when any individual presents with symptoms of a recreational party drug overdose.

  4. When Parties become Racialized: Deconstructing Racially Themed Parties

    Science.gov (United States)

    Garcia, Gina A.; Johnston, Marc P.; Garibay, Juan C.; Herrera, Felisha A.; Giraldo, Luis G.

    2011-01-01

    Racially themed parties are all-too-common occurrences on college campuses. Using critical race theory as a lens, this article provides a contemporary overview of these events and deconstructs these incidents as examples of overt forms of racism often emanating from subtle, everyday occurrences of covert racism or racial microaggressions.…

  5. Bewitched - The Tea Party Movement

    DEFF Research Database (Denmark)

    Ashbee, Edward

    2011-01-01

    This article considers the development of the Tea Party movement, the character of its thinking and the nature of the interests and constituencies to which it is tied. The article suggests that despite the importance of ideas and interests, and the process of interaction between them, the movement....... The political friction that this creates has contributed to the anger that has characterised the movement. While the Tea Party movement may, as such, have only an ephemeral existence, independent conservatives are likely to remain a significant and potent constituency and will, within the institutional...

  6. Public Funding of Political Parties

    DEFF Research Database (Denmark)

    Ortuno-Ortin, Ignacio; Schultz, Christian

    This paper concerns public funding of parties. Parteis receive public funds depending on their vote share. Funds finance electoral campaigns. Two cases are investigated. In the first, some voters are policy motivated and some are ?impressionable? ? their vote depends directly on campaign...... expenditures. In the second, campaigning is informative and all voters are policy motivated. Public funds increase policy convergence in both cases. The effect is larger, the more funding depends on vote shares. When campaigns are informative, there may be multiple euqilibria. Intuitively, a large party can...... stay large since it receives large funds...

  7. Public Funding of Political Parties

    DEFF Research Database (Denmark)

    Ortuno-Ortin, Ignacio; Schultz, Christian

    expenditures. In the second, campaigning is informative and all voters are policy motivated. Public funds increase policy convergence in both cases. The effect is larger, the more funding depends on vote shares. When campaigns are informative, there may be multiple euqilibria. Intuitively, a large party can......This paper concerns public funding of parties. Parteis receive public funds depending on their vote share. Funds finance electoral campaigns. Two cases are investigated. In the first, some voters are policy motivated and some are ?impressionable? ? their vote depends directly on campaign...

  8. Selective Attention Enhances Beta-Band Cortical Oscillation to Speech under “Cocktail-Party” Listening Conditions

    Science.gov (United States)

    Gao, Yayue; Wang, Qian; Ding, Yu; Wang, Changming; Li, Haifeng; Wu, Xihong; Qu, Tianshu; Li, Liang

    2017-01-01

    Human listeners are able to selectively attend to target speech in a noisy environment with multiple-people talking. Using recordings of scalp electroencephalogram (EEG), this study investigated how selective attention facilitates the cortical representation of target speech under a simulated “cocktail-party” listening condition with speech-on-speech masking. The result shows that the cortical representation of target-speech signals under the multiple-people talking condition was specifically improved by selective attention relative to the non-selective-attention listening condition, and the beta-band activity was most strongly modulated by selective attention. Moreover, measured with the Granger Causality value, selective attention to the single target speech in the mixed-speech complex enhanced the following four causal connectivities for the beta-band oscillation: the ones (1) from site FT7 to the right motor area, (2) from the left frontal area to the right motor area, (3) from the central frontal area to the right motor area, and (4) from the central frontal area to the right frontal area. However, the selective-attention-induced change in beta-band causal connectivity from the central frontal area to the right motor area, but not other beta-band causal connectivities, was significantly correlated with the selective-attention-induced change in the cortical beta-band representation of target speech. These findings suggest that under the “cocktail-party” listening condition, the beta-band oscillation in EEGs to target speech is specifically facilitated by selective attention to the target speech that is embedded in the mixed-speech complex. The selective attention-induced unmasking of target speech may be associated with the improved beta-band functional connectivity from the central frontal area to the right motor area, suggesting a top-down attentional modulation of the speech-motor process. PMID:28239344

  9. Antisense oligonucleotide induced exon skipping and the dystrophin gene transcript: cocktails and chemistries

    Directory of Open Access Journals (Sweden)

    Fletcher Sue

    2007-07-01

    Full Text Available Abstract Background Antisense oligonucleotides (AOs can interfere with exon recognition and intron removal during pre-mRNA processing, and induce excision of a targeted exon from the mature gene transcript. AOs have been used in vitro and in vivo to redirect dystrophin pre-mRNA processing in human and animal cells. Targeted exon skipping of selected exons in the dystrophin gene transcript can remove nonsense or frame-shifting mutations that would otherwise have lead to Duchenne Muscular Dystrophy, the most common childhood form of muscle wasting. Results Although many dystrophin exons can be excised using a single AO, several exons require two motifs to be masked for efficient or specific exon skipping. Some AOs were inactive when applied individually, yet pronounced exon excision was induced in transfected cells when the AOs were used in select combinations, clearly indicating synergistic rather than cumulative effects on splicing. The necessity for AO cocktails to induce efficient exon removal was observed with 2 different chemistries, 2'-O-methyl modified bases on a phosphorothioate backbone and phosphorodiamidate morpholino oligomers. Similarly, other trends in exon skipping, as a consequence of 2'-O-methyl AO action, such as removal of additional flanking exons or variations in exon skipping efficiency with overlapping AOs, were also seen when the corresponding sequences were prepared as phosphorodiamidate morpholino oligomers. Conclusion The combination of 2 AOs, directed at appropriate motifs in target exons was found to induce very efficient targeted exon skipping during processing of the dystrophin pre-mRNA. This combinatorial effect is clearly synergistic and is not influenced by the chemistry of the AOs used to induce exon excision. A hierarchy in exon skipping efficiency, observed with overlapping AOs composed of 2'-O-methyl modified bases, was also observed when these same sequences were evaluated as phosphorodiamidate morpholino

  10. 27 CFR 31.233 - Mixing cocktails in advance of sale.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Mixing cocktails in advance of sale. 31.233 Section 31.233 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND... liquors in advance of sale, except for the purpose of filling, for immediate consumption on the...

  11. Yeast mediates lactic acidosis suppression after antibiotic cocktail treatment in short small bowel?

    NARCIS (Netherlands)

    Bongaerts, G.P.A.; Severijnen, R.S.V.M.; Skladal, D.; Bakkeren, J.A.J.; Sperl, W.

    2005-01-01

    During acidotic periods in a girl with a short small bowel, very high D-lactic acid concentrations were measured in blood and urine; the patient's characteristic faecal flora contained mainly lactobacilli, and during antibiotic cocktail treatment also many yeasts. In this case report we sought to un

  12. Determination of polychlorinated biphenyls (PCBs) in insulating oil by cocktail PCB ELISA

    Energy Technology Data Exchange (ETDEWEB)

    Goda, Y.; Hirobe, M.; Fujimoto, S. [Japan EnviroChemicals Ltd., Tokyo (Japan); Tomita, J.; Nishikiori, M.; Nakajima, T. [Tokyo Electric Power Environmental Engineering Co. Inc., Tokyo (Japan); Rubio, F. [Abraxis LLC, Warminster, PA (United States); Takigami, H. [National Inst. for Environmental Studies, Tsukuba (Japan); Sakai, S. [Kyoto Univ., Kyoto (Japan). Environment Preservation Centre; Ike, M.; Fujita, M. [Osaka Univ., Osaka (Japan). Graduate School of Engineering

    2005-07-01

    This paper described a novel cocktail polychlorinated biphenyl (PCB) enzyme-linked immunosorbent assay (ELISA) designed as a simple pretreatment method for the determination of PCBs in insulating oils. Oil samples were treated with hexane and dimethyl sufloxide (DMSO) and mixed. Hexane layers were then removed, and freshly prepared hexane was added. The DMSO layer was then diluted with an aqueous solution and extracted with hexane. After being dehydrated, the hexane layer was sulfonated with a sulfuric reagent and washed. Standard or pretreated PCBs were then dissolved and diluted in 100 per cent DMSO and added to distilled water prior to the ELISA assay. A horseradish peroxidase conjugate and an anti-PCB antibody coupled with magnetic particles were added for the assay. A magnetic rack was used to separate the reaction mixture. A colouring reagent was then added. A total of 26 insulating oils contaminated with PCBs were determined using the new cocktail method and compared with data obtained from traditional gas chromatograph electron capture detector (GC-ECD) data. Results showed that the values of both the cocktail and general PCB ELISA were well correlated with the CG-ECD data. It was concluded that combining cocktail PCB ELISA with the developed pretreatment method provided accurate results. 3 refs., 4 tabs., 2 figs.

  13. Preparation of multi-locus DNA probe cocktail by liquid-phase reassociation.

    Science.gov (United States)

    Tamaki, Y; Fukuda, M; Kishida, T; Wang, W

    1995-08-01

    We developed a simple, rapid method for the preparation of a DNA-fingerprinting probe cocktail, and tested its usefulness in paternity testing. Exploiting the property of tandemly repetitive DNA segments to be rapidly renatured after heat denaturation, we enriched restriction fragments of a child's genomic DNA for minisatellites by liquid phase reassociation followed by capture with immobilized streptavidin. We amplified and simultaneously labeled the reassociation product by anchored PCR using a digoxigenin-labeling mixture. Using this probe cocktail, we were able to detect fingerprints of paternity case trios, and the results were corroborated by DNA fingerprinting with a commercially available probe as well as by conventional phenotyping. Our method enables one to prepare a fresh cocktail of probes from the DNA sample under study during the overnight electrophoresis and Southern transfer steps in DNA fingerprinting, and eliminates the need of having an expensive probe of limited shelf life. If one has a practical outlook on DNA fingerprinting and regard it as a preliminary test, one does not have to use a cloned DNA probe. The present study demonstrates that a multi-locus probe cocktail serves such a practical purpose.

  14. [Dendritic cells (DC) induced from acute myeloid leukemia (AML) cells with cytokine cocktails].

    Science.gov (United States)

    Yan, Kuang-hua; You, Sheng-guo; Bian, Shou-geng; Ma, Guan-jie; Ge, Wei; Ma, Shuang; Liu, Shi-he; Zhao, Chun-hua

    2003-07-01

    To explore the feasibility of DC being in vitro induced from AML cells with cytokine cocktails and their biological properties. AML cells were cultured in either presence or absence of cytokine cocktails. DC were studied for morphology, and cytochemical and immunofluorescent staining. Functions of DC were examined by MLC, FITC-conjugated dextran uptake test, and LDH release assay. RT-PCR and FISH were used to analyze the specific fusion genes of culture-derived DC. Classical DC morphological changes occurred in all 15 cultured AML cells. DC-associated surface molecules such as CD(1a), CD(80), CD(86), CD(106), CD(83) and HLA-DR were upregulated (P AML cells uncultured or cultured in the absence of cytokines (P CTL assay was performed in 5 of the 15 samples. At effector/target ratio of 20:1, auto-T lymphocytes primed with the culture-derived DC exhibited no more killing activity to auto-AML cells than those stimulated by IL-2 or uncultured AML cells. Culture-derived DC presenced the native AML-specific aberrant karyotype and related fusion gene. Cytokine cocktails could in vitro induce AML cells into DC with classical morphology, immunophenotype and function. DC maturity induced by different cytokine cocktails could be variable. Culture-derived DC were originated from the native AML cells. AML cells could make the auto-T lymphocyte anergy.

  15. Development of minimal enzyme cocktails for hydrolysis of sulfite-pulped lignocellulosic biomass.

    Science.gov (United States)

    Chylenski, Piotr; Forsberg, Zarah; Ståhlberg, Jerry; Várnai, Anikó; Lersch, Martin; Bengtsson, Oskar; Sæbø, Solve; Horn, Svein Jarle; Eijsink, Vincent G H

    2017-03-20

    Despite recent progress, saccharification of lignocellulosic biomass is still a major cost driver in biorefining. In this study, we present the development of minimal enzyme cocktails for hydrolysis of Norway spruce and sugarcane bagasse, which were pretreated using the so-called BALI™ process, which is based on sulfite pulping technology. Minimal enzyme cocktails were composed using several glycoside hydrolases purified from the industrially relevant filamentous fungus Trichoderma reesei and a purified commercial β-glucosidase from Aspergillus niger. The contribution of in-house expressed lytic polysaccharide monooxygenases (LPMOs) was also tested, since oxidative cleavage of cellulose by such LPMOs is known to be beneficial for conversion efficiency. We show that the optimized cocktails permit efficient saccharification at reasonable enzyme loadings and that the effect of the LPMOs is substrate-dependent. Using a cocktail comprising only four enzymes, glucan conversion for Norway spruce reached >80% at enzyme loadings of 8mg/g glucan, whereas almost 100% conversion was achieved at 16mg/g. Copyright © 2017 Elsevier B.V. All rights reserved.

  16. Podemos som postkrise-parti

    DEFF Research Database (Denmark)

    Agustin, Oscar Garcia

    2015-01-01

    Podemos er et nyt politisk parti i Spanien, stiftet efter finanskrisen. Partiet har allerede opnået stor tilslutning og forventes at komme til at spille en rolle i spansk politik efter det nationale valg i efteråret 2015. Podemos repræsenterer en ny type venstrefløjsparti med tætte relationer til...

  17. YOUNG CITY,BIG PARTY

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Shenzhen Universiade unites the world’s young people through sports with none of the usual hoop-la, no fireworks, no grand performances by celebrities and superstars, the Shenzhen Summer Universiade lowered the curtain on a big party for youth and college students on August 23.

  18. Which Party Gets the Mayoralty?

    DEFF Research Database (Denmark)

    Skjæveland, Asbjørn; Serritzlew, Søren

    2010-01-01

    In non-presidential multiparty systems, it is not only the voters but also coalition formation after the election that decides the government of the community. Some national-level studies investigate which parties are most likely to win the Prime Minister's office. The aim of this article...

  19. Which Party Gets the Mayoralty?

    DEFF Research Database (Denmark)

    Skjæveland, Asbjørn; Serritzlew, Søren

    2010-01-01

    In non-presidential multiparty systems, it is not only the voters but also coalition formation after the election that decides the government of the community. Some national-level studies investigate which parties are most likely to win the Prime Minister's office. The aim of this article...

  20. [Ketamine as a party drug

    NARCIS (Netherlands)

    Vroegop, M.P.; Dongen, R.T.M. van; Troyen, B. van; Kramers, C.

    2007-01-01

    Ketamine is a new party drug, which is easy to obtain. For this reason, it is possible that physicians will be increasingly confronted with users that have medical problems. Relatively few cases of ketamine intoxication with a fatal outcome have been reported thus far. Ketamine is very

  1. [Ketamine as a party drug

    NARCIS (Netherlands)

    Vroegop, M.P.; Dongen, R.T.M. van; Troyen, B. van; Kramers, C.

    2007-01-01

    Ketamine is a new party drug, which is easy to obtain. For this reason, it is possible that physicians will be increasingly confronted with users that have medical problems. Relatively few cases of ketamine intoxication with a fatal outcome have been reported thus far. Ketamine is very hallucinogeni

  2. Population policy: major party positions.

    Science.gov (United States)

    Betts, K

    1998-01-01

    This article identifies the major political party positions on population policy (PP) in Australia. Australia has the Governing Coalition, comprised of the Liberal and National Parties, and the Australian Labor Party (ALP), the main opposition party. The ALP adopted a PP at its national conference in 1998. The Government Minister for Immigration and Multicultural Affairs (DIMA) does not view a PP as a need. The ALP policy identifies the need to decide on a long term sustainable population size as determined within a PP framework. The visa classification system needs to reflect national priorities. Labor will support a fair refugee and humanitarian program. Skilled labor migration must be linked with labor market needs. Business migration is a means of transferring resources and technology to Australia. The DIMA Minister announced the target intake of 80,000 visa immigrants in 1998- 99, which would produce a peak population of about 23 million in 2050. Settler arrivals from New Zealand would increase population size. A group of scientists and scholars published their recommendations about development of a PP. The Minister of DIMA's defense of the lack of a PP is included in this publication. Labor in several publications indicates support for a larger immigration intake, or an adjustment of the intake within the existing numbers. The Prime Minister, who supports the concept of multiculturalism, argues that people must accept immigration policy. Politics today are more divided over the origin and future of the nation than population size issues.

  3. Polyclonal antibody cocktails generated using DNA vaccine technology protect in murine models of orthopoxvirus disease

    Directory of Open Access Journals (Sweden)

    Ballantyne John

    2011-09-01

    Full Text Available Abstract Background Previously we demonstrated that DNA vaccination of nonhuman primates (NHP with a small subset of vaccinia virus (VACV immunogens (L1, A27, A33, B5 protects against lethal monkeypox virus challenge. The L1 and A27 components of this vaccine target the mature virion (MV whereas A33 and B5 target the enveloped virion (EV. Results Here, we demonstrated that the antibodies produced in vaccinated NHPs were sufficient to confer protection in a murine model of lethal Orthopoxvirus infection. We further explored the concept of using DNA vaccine technology to produce immunogen-specific polyclonal antibodies that could then be combined into cocktails as potential immunoprophylactic/therapeutics. Specifically, we used DNA vaccines delivered by muscle electroporation to produce polyclonal antibodies against the L1, A27, A33, and B5 in New Zealand white rabbits. The polyclonal antibodies neutralized both MV and EV in cell culture. The ability of antibody cocktails consisting of anti-MV, anti-EV, or a combination of anti-MV/EV to protect BALB/c mice was evaluated as was the efficacy of the anti-MV/EV mixture in a mouse model of progressive vaccinia. In addition to evaluating weight loss and lethality, bioimaging technology was used to characterize the spread of the VACV infections in mice. We found that the anti-EV cocktail, but not the anti-MV cocktail, limited virus spread and lethality. Conclusions A combination of anti-MV/EV antibodies was significantly more protective than anti-EV antibodies alone. These data suggest that DNA vaccine technology could be used to produce a polyclonal antibody cocktail as a possible product to replace vaccinia immune globulin.

  4. 论党政干部品德测评的人力资源开发功能%On the Developing Function of Human Resource for Morality Testing of Party and Governmental Cadres

    Institute of Scientific and Technical Information of China (English)

    曹伟晓

    2016-01-01

    This paper briefly reviews moral evaluation studies of the party and government cadres that there are some accomplishments, and also some challenges which is focused on reliability and validity issues,efficiency,as well as insufficient function of development.On the premise of fuzzy evaluation and careful guidance,this paper presents some current practice issues:the cadres make moral evaluation play selection functions,but often overlooked the educational functions,which is embodied in the consciousness of party and government cadres is not strong for human resource development functions of moral evaluation,supporting system is not perfect and the system is not strong.Thus the paper proposes an improved method:to raise awareness, support the establishment of institutional mechanisms,enhance systematic.The paper illustrated how to strengthen the effectiveness of the moral development from political morality,professional morality and morality outside of duties.The conclusion is the development trend of modern moral evaluation of management functions must be gradually diluted, increasingly strengthen the development function,which requires a series of reforms in the evaluation of the content and method.%党政干部品德测评现行实践发挥了管理性功能,但经常忽略了开发性功能的拓展。具体体现在党政干部对品德测评的人力资源开发功能的意识不强、观念滞后,配套体制机制不完善,系统性不强。通过增强发挥品德测评人力资源开发功能的意识,建立配套体制机制并增强开发的系统性,改善测评的信效度、效率,在完善管理性功能的基础上,日益强化品德测评的开发性功能。

  5. Party rules, party resources and the politics of parliamentary democracies: How parties organize in the 21st century

    DEFF Research Database (Denmark)

    Poguntke, Thomas; Scarrow, Susan; Webb, Paul;

    2016-01-01

    This article introduces the first findings of the Political Party Database Project, a major survey of party organizations in parliamentary and semi-presidential democracies. The project’s first round of data covers 122 parties in 19 countries. In this article, we describe the scope of the database...

  6. Joe Zhang, Party Man, Company Man

    DEFF Research Database (Denmark)

    Brødsgaard, Kjeld Erik

    2014-01-01

    Book review of: Joe Zhang: Party Man, Company Man. Honolulu: Enrich Professional Publishing, 2014. 234 pp.......Book review of: Joe Zhang: Party Man, Company Man. Honolulu: Enrich Professional Publishing, 2014. 234 pp....

  7. Third-Party Cooperation: How Reducing Material Involvement Enhances Contributions to the Public Good.

    Science.gov (United States)

    Losecaat Vermeer, Annabel B; Heerema, Roeland L; Sanfey, Alan G

    2016-03-01

    Decisions to cooperate are often delegated to a third party. We examined whether cooperation differs when decisions are made for a third party compared with ourselves and specified which motives are important for third-party cooperation. Participants played multiple rounds of a public goods game (PGG). In Study 1, we varied personal involvement from high to low; participants played for themselves (Self), for themselves and a third party (Shared), and solely for a third party (Third Party). Participants contributed most when personal involvement was lowest (i.e., Third Party) and least when personal involvement was high (i.e., Self). Study 2 explored if social motives underlie third-party cooperation by comparing cooperation with social (human) and non-social (computer) group members. Reducing personal involvement in the PGG (i.e., Third Party) increased cooperation in social contexts compared with non-social contexts, indicating enhanced collective interest. Increased cooperation for a third party may result from taking the other's perspective, thereby increasing social norm preferences. © 2016 by the Society for Personality and Social Psychology, Inc.

  8. In vitro evaluation of a novel bacteriophage cocktail as a preventative for bovine coliform mastitis.

    Science.gov (United States)

    Porter, J; Anderson, J; Carter, L; Donjacour, E; Paros, M

    2016-03-01

    The objective of this study was to investigate the potential use of bacteriophage in preventing Escherichia coli mastitis on dairies. A cocktail consisting of 4 distinct bacteriophages was generated by screening against 36 E. coli isolates from dairy cows in Washington State with clinical mastitis. The bacteriophage significantly inhibited growth of 58% of the Washington State isolates and 54% of E. coli mastitis isolates from New York State, suggesting that the cocktail of phages had a relatively broad spectrum of action against relevant strains from 2 distinct geographies. The ability to suppress bacterial growth of these isolates in a liquid growth medium was not affected by the ratio of bacteriophage particles to bacterial cells (multiplicity of infection, MOI). For those E. coli that were completely inhibited by the phage cocktail, an MOI as low as 10 had the same effect as 10 µg/mL of ceftiofur on the growth rate of E. coli over a 12-h period using optical density measurements. A 3.3- to 5.6-log reduction of growth was achieved when E. coli was co-incubated with our phage cocktail in raw milk over a 12-h period at physiologic temperature. A modified gentamicin protection assay using bovine mammary epithelial cells provided a model to test whether bacteriophage could prevent cell attachment and invasion by chronic coliform mastitis strains. Pretreatment of cell cultures with the phage cocktail significantly reduced adhesion and intracellular survival of E. coli compared with controls. When combined with a bismuth-based teat sealant, the phage cocktail was able to inhibit bacterial growth when challenged with 1.6 × 10(3) cfu/mL of a clinical mastitis E. coli strain. In vitro results show bactericidal activity by our phage in raw milk and mammary tissue culture systems. Before a bacteriophage-based dry-cow treatment becomes a potential option for dairies, in vivo studies must be able to demonstrate that a specific dose of bacteriophage can protect cows from

  9. Party System Compactness: Measurement and Consequences

    NARCIS (Netherlands)

    Alvarez, R. Michael; Nagler, Jonathan

    2004-01-01

    An important property of any party system is the set of choices it presents to the electorate. In this paper we analyze the distribution of parties relative to voters in the multidimensional issue space and introduce two measures of the dispersion of the parties in the issue space relative to the vo

  10. Patronage and party organization in Argentina : the emergence of the patronage-based network party

    NARCIS (Netherlands)

    Scherlis Perel, Gerardo Ezequiel

    2010-01-01

    This dissertation consists of a study of party patronage in Argentina. It attempts to assess the degree to which parties appoint people to public positions, who is in effect responsible for patronage within parties, what motivates parties to appoint in different sectors and at different levels of th

  11. Do Mainstream Parties Adapt to the Welfare Chauvinism of Populist Parties?

    DEFF Research Database (Denmark)

    Schumacher, Gijs; van Kersbergen, Kees

    2016-01-01

    and when mainstream parties ignore, attack or accommodate welfare chauvinism. Using key theories of party behaviour, we test whether mainstream parties (1) respond immediately to populist parties, (2) respond with a time lag, or (3) respond only when they lose elections or are in opposition. Our...

  12. Patronage and party organization in Argentina : the emergence of the patronage-based network party

    NARCIS (Netherlands)

    Scherlis Perel, Gerardo Ezequiel

    2010-01-01

    This dissertation consists of a study of party patronage in Argentina. It attempts to assess the degree to which parties appoint people to public positions, who is in effect responsible for patronage within parties, what motivates parties to appoint in different sectors and at different levels of

  13. Anticancer activity of an extract from needles and twigs of Taxus cuspidata and its synergistic effect as a cocktail with 5-fluorouracil

    Directory of Open Access Journals (Sweden)

    Shang Weihu

    2011-12-01

    Full Text Available Abstract Background Botanical medicines are increasingly combined with chemotherapeutics as anticancer drug cocktails. This study aimed to assess the chemotherapeutic potential of an extract of Taxus cuspidata (TC needles and twigs produced by artificial cuttage and its co-effects as a cocktail with 5-fluorouracil (5-FU. Methods Components of TC extract were identified by HPLC fingerprinting. Cytotoxicity analysis was performed by MTT assay or ATP assay. Apoptosis studies were analyzed by H & E, PI, TUNEL staining, as well as Annexin V/PI assay. Cell cycle analysis was performed by flow cytometry. 5-FU concentrations in rat plasma were determined by HPLC and the pharmacokinetic parameters were estimated using 3p87 software. Synergistic efficacy was subjected to median effect analysis with the mutually nonexclusive model using Calcusyn1 software. The significance of differences between values was estimated by using a one-way ANOVA. Results TC extract reached inhibition rates of 70-90% in different human cancer cell lines (HL-60, BGC-823, KB, Bel-7402, and HeLa but only 5-7% in normal mouse T/B lymphocytes, demonstrating the broad-spectrum anticancer activity and low toxicity to normal cells of TC extract in vitro. TC extract inhibited cancer cell growth by inducing apoptosis and G2/M cell cycle arrest. Most interestingly, TC extract and 5-FU, combined as a cocktail, synergistically inhibited the growth of cancer cells in vitro, with Combination Index values (CI ranging from 0.90 to 0.26 at different effect levels from IC50 to IC90 in MCF-7 cells, CI ranging from 0.93 to 0.13 for IC40 to IC90 in PC-3M-1E8 cells, and CI TC extract did not affect the pharmacokinetics of 5-FU in rats. Conclusions The combinational use of the TC extract with 5-FU displays strong cytotoxic synergy in cancer cells and low cytotoxicity in normal cells. These findings suggest that this cocktail may have a potential role in cancer treatment.

  14. The in vivo efficacy of two administration routes of a phage cocktail to reduce numbers of Campylobacter coli and Campylobacter jejuni in chickens

    Directory of Open Access Journals (Sweden)

    Carvalho Carla M

    2010-09-01

    Full Text Available Abstract Background Poultry meat is one of the most important sources of human campylobacteriosis, an acute bacterial enteritis which is a major problem worldwide. Campylobacter coli and Campylobacter jejuni are the most common Campylobacter species associated with this disease. These pathogens live in the intestinal tract of most avian species and under commercial conditions they spread rapidly to infect a high proportion of the flock, which makes their treatment and prevention very difficult. Bacteriophages (phages are naturally occurring predators of bacteria with high specificity and also the capacity to evolve to overcome bacterial resistance. Therefore phage therapy is a promising alternative to antibiotics in animal production. This study tested the efficacy of a phage cocktail composed of three phages for the control of poultry infected with C. coli and C. jejuni. Moreover, it evaluated the effectiveness of two routes of phage administration (by oral gavage and in feed in order to provide additional information regarding their future use in a poultry unit. Results The results indicate that experimental colonisation of chicks was successful and that the birds showed no signs of disease even at the highest dose of Campylobacter administered. The phage cocktail was able to reduce the titre of both C. coli and C. jejuni in faeces by approximately 2 log10 cfu/g when administered by oral gavage and in feed. This reduction persisted throughout the experimental period and neither pathogen regained their former numbers. The reduction in Campylobacter titre was achieved earlier (2 days post-phage administration when the phage cocktail was incorporated in the birds' feed. Campylobacter strains resistant to phage infection were recovered from phage-treated chickens at a frequency of 13%. These resistant phenotypes did not exhibit a reduced ability to colonize the chicken guts and did not revert to sensitive types. Conclusions Our findings provide

  15. Therapeutic effect of Lianbeijuqin (a Chinese herbal cocktail) on ...

    African Journals Online (AJOL)

    significantly decreased GI and AL (p < 0.05) in the periodontitis model rats. ... Animals. Sprague Dawley (SD) rats (260 ~ 280 g) were got from Shanghai Laboratory Animal ..... development of a new and effective drug from ... and reproduction in any medium, provided the ... Degradation in LPS-Stimulated Human Periodontal.

  16. Prospective randomized study of viscous lidocaine versus benzocaine in a GI cocktail for dyspepsia.

    Science.gov (United States)

    Vilke, Gary M; Jin, Albert; Davis, Daniel P; Chan, Theodore C

    2004-07-01

    We hypothesized that Benzocaine (Hurricaine) would work as quickly and effectively as viscous Lidocaine in this preparation. This was a prospective randomized, single-blinded comparison between Benzocaine and Lidocaine as the topical anesthetic in a gastrointestinal (GI) cocktail. Patients 18 years or older were approached for participation when a GI cocktail was ordered by the Emergency Physician. Patients were randomized to equivalent doses of either Benzocaine or viscous Lidocaine in addition to 30 cc of Maalox and 10 cc of Donnatal. Assessment using a visual analog pain scale occurred at time intervals of 0, 5, 15, and 30 min. Eighty-two patients were enrolled (44 to Benzocaine, 38 to viscous Lidocaine), with each group having a statistically significant improvement in pain (p Benzocaine and viscous Lidocaine groups in terms of the relief of symptoms at each of the assessment times. There were no adverse outcomes in either group.

  17. Anionic markers for the forensic identification of Chemical Ignition Molotov Cocktail composition.

    Science.gov (United States)

    Martín-Alberca, C; Ferrando, J L; García-Ruiz, C

    2013-03-01

    An improved version of the famous Molotov cocktail is the Chemical Ignition Molotov Cocktail (CIMC). This incendiary device contains chemical reagents that enable its self-ignition. The analysis of anions from CIMC residues by capillary electrophoresis (CE) allows the identification of the reagents used to produce the device, and provides forensic analysts with valuable information. Although, sulfate, chlorate, chloride, and perchlorate anions have been recently proposed in the literature as target anions to determine the CIMC composition, the identification of some of them could be controversial due to their presence in the environment. Therefore, the purpose of this study was to identify highly reliable anions capable of indicating the components used to prepare these self-initiated devices. The relationship among the detected anions in CIMC residues and the reagents employed in their elaboration is discussed. Some anions have been proposed as anionic markers of CIMC as incendiary devices. Additionally, the viability of different CIMC compositions was studied.

  18. Effect of a Thermoascus aurantiacus thermostable enzyme cocktail on wheat bread qualitiy.

    Science.gov (United States)

    Oliveira, D S; Telis-Romero, J; Da-Silva, R; Franco, C M L

    2014-01-15

    Thermophilic fungus Thermoascus aurantiacus (CBMAI 756) on solid-state fermentation using corncob as a nutrient source produces an enzyme pool with the potential to be used in bread making. In this paper, the use of this enzyme cocktail as a wheat bread improver was reported. Both products released by flour arabinoxylan degradation and bread quality were investigated. The main product released through enzyme activity after prolonged incubation was xylose indicating the presence of xylanase; however, a small amount of xylobiose and arabinose also confirmed the presence of xylosidase and α-l-arabinofuranosidase, respectively. Enzyme mixture "in vitro" mainly attacked water-unextractable arabinoxylan contributing to beneficial effect in bread making. The use of an optimal enzyme concentration (35U xylanase/100g of flour) increased specific volume (22%), reduced crumb firmness (25%), and reduced amylopectin retrogradation (17%) during bread storage. In conclusion, the enzyme cocktail produced by T. aurantiacus CBMAI 756 can improve wheat bread quality.

  19. Bacteriophages with potential to inactivate Salmonella Typhimurium: Use of single phage suspensions and phage cocktails.

    Science.gov (United States)

    Pereira, Carla; Moreirinha, Catarina; Lewicka, Magdalena; Almeida, Paulo; Clemente, Carla; Cunha, Ângela; Delgadillo, Ivonne; Romalde, Jésus L; Nunes, Maria L; Almeida, Adelaide

    2016-07-15

    The aim of this study was to compare the dynamics of three previously isolated bacteriophages (or phages) individually (phSE-1, phSE-2 and phSE-5) or combined in cocktails of two or three phages (phSE-1/phSE-2, phSE-1/phSE-5, phSE-2/phSE-5 and phSE-1/phSE-2/phSE-5) to control Salmonella enterica serovar Typhimurium (Salmonella Typhimurium) in order to evaluate their potential application during depuration. Phages were assigned to the family Siphoviridae and revealed identical restriction digest profiles, although they showed a different phage adsorption, host range, burst size, explosion time and survival in seawater. The three phages were effective against S. Typhimurium (reduction of ∼2.0 log CFU/mL after 4h treatment). The use of cocktails was not significantly more effective than the use of single phages. A big fraction of the remained bacteria are phage-resistant mutants (frequency of phage-resistant mutants 9.19×10(-5)-5.11×10(-4)) but phage- resistant bacterial mutants was lower for the cocktail phages than for the single phage suspensions and the phage phSE-1 presented the highest rate of resistance and phage phSE-5 the lowest one. The spectral changes of S. Typhimurium resistant and phage-sensitive cells were compared and revealed relevant differences for peaks associated to amide I (1620cm(-1)) and amide II (1515cm(-1)) from proteins and from carbohydrates and phosphates region (1080-1000cm(-1)). Despite the similar efficiency of individual phages, the development of lower resistance indicates that phage cocktails might be the most promising choice to be used during the bivalve depuration to control the transmission of salmonellosis.

  20. Antibody Production in Response to Staphylococcal MS-1 Phage Cocktail in Patients Undergoing Phage Therapy

    OpenAIRE

    Maciej Żaczek; Marzanna Łusiak-Szelachowska; Ewa Jończyk-Matysiak; Beata Weber-Dąbrowska; Ryszard Międzybrodzki; Barbara Owczarek; Agnieszka Kopciuch; Wojciech Fortuna; Paweł Rogóż; Andrzej Górski

    2016-01-01

    In this study, we investigated the humoral immune response (through the release of IgG, IgA, and IgM antiphage antibodies) to a staphylococcal phage cocktail in patients undergoing experimental phage therapy at the Phage Therapy Unit, Medical Center of the Ludwik Hirszfeld Institute of Immunology and Experimental Therapy in Wrocław, Poland. We also evaluated whether occurring antiphage antibodies had neutralizing properties towards applied phages (K rate). Among 20 examined patients receiving...

  1. The Chinese Communist Party in Reform

    DEFF Research Database (Denmark)

    Brødsgaard, Kjeld Erik; Yongnian, Zheng

    Contrary to the expectations of many people, China's recent economic growth has not led to the collapse of the Chinese Communist Party. In fact, the party has recently carried out a peaceful and orderly transition to the so-called fourth generation of leadership, has revitalised itself, and created...... social groups, and increasing dissatisfaction among workers who seem to be losing out in the present transition process. "The Chinese Communist Party in Reform" explores the current state of the Chinese Communist Party and the many challenges which it faces. It considers the dynamics of development...... in China, the party organisation, recruitment and management, and the party's role in society more widely. It concludes by examining the prospects for the future of the Party, including whether it will continue to be able to accommodate socio-economic changes within China and pressures from abroad...

  2. Root exudate cocktails: the link between plant diversity and soil microorganisms?

    Science.gov (United States)

    Steinauer, Katja; Chatzinotas, Antonis; Eisenhauer, Nico

    2016-10-01

    Higher plant diversity is often associated with higher soil microbial biomass and diversity, which is assumed to be partly due to elevated root exudate diversity. However, there is little experimental evidence that diversity of root exudates shapes soil microbial communities. We tested whether higher root exudate diversity enhances soil microbial biomass and diversity in a plant diversity gradient, thereby negating significant plant diversity effects on soil microbial properties. We set up plant monocultures and two- and three-species mixtures in microcosms using functionally dissimilar plants and soil of a grassland biodiversity experiment in Germany. Artificial exudate cocktails were added by combining the most common sugars, organic acids, and amino acids found in root exudates. We applied four different exudate cocktails: two exudate diversity levels (low- and high-diversity) and two nutrient-enriched levels (carbon- and nitrogen-enriched), and a control with water only. Soil microorganisms were more carbon- than nitrogen-limited. Cultivation-independent fingerprinting analysis revealed significantly different soil microbial communities among exudate diversity treatments. Most notably and according to our hypothesis, adding diverse exudate cocktails negated the significant plant diversity effect on soil microbial properties. Our findings provide the first experimental evidence that root exudate diversity is a crucial link between plant diversity and soil microorganisms.

  3. The intravesicular cocktail and its role in the regulation of exocytosis.

    Science.gov (United States)

    Estévez-Herrera, Judith; González-Santana, Ayoze; Baz-Dávila, Rebeca; Machado, José D; Borges, Ricardo

    2016-06-01

    The accumulation of neurotransmitters within secretory vesicles (SVs) far exceeds the theoretical tonic concentrations in the cytosol, a phenomenon that has captivated the attention of scientists for decades. For instance, chromaffin granules can accumulate close to molar concentrations of catecholamines, along with many other products like ATP, calcium, peptides, chromogranins, ascorbate, and other nucleotides. In this short review, we will summarize the interactions that are currently believed to occur between the elements that make up the vesicular cocktail in the acidic environment of SVs, and how they permit the accumulation of such high concentrations of certain components. In addition, we will examine how the vesicular cocktail regulates the exocytosis of neurotransmitters. In this review, we have highlighted the mechanisms that permit the storage of neurotransmitters and hormones inside secretory vesicles. We also have proposed a novel model based in the intravesicular interactions of the main components of this inner cocktail - catecholamines, ATP, and chromogranins - to allow the accumulation of near molar concentrations of transmitters in secretory vesicles. This article is part of a mini review series on Chromaffin cells (ISCCB Meeting, 2015).

  4. Production of cocktail of polyclonal antibodies using bacterial expressed recombinant protein for multiple virus detection.

    Science.gov (United States)

    Kapoor, Reetika; Mandal, Bikash; Paul, Prabir Kumar; Chigurupati, Phaneendra; Jain, Rakesh Kumar

    2014-02-01

    Cocktail of polyclonal antibodies (PAb) were produced that will help in multiple virus detection and overcome the limitation of individual virus purification, protein expression and purification as well as immunization in multiple rabbits. A dual fusion construct was developed using conserved coat protein (CP) sequences of Cucumber mosaic virus (CMV) and Papaya ringspot virus (PRSV) in an expression vector, pET-28a(+). The fusion protein (∼40kDa) was expressed in Escherichia coli and purified. Likewise, a triple fusion construct was developed by fusing conserved CP sequences of CMV and PRSV with conserved nucleocapsid protein (N) sequence of Groundnut bud necrosis virus (GBNV) and expressed as a fusion protein (∼50kDa) in pET-28a(+). PAb made separately to each of these three viruses recognized the double and triple fusion proteins in Western blot indicating retention of desired epitopes for binding with target antibodies. The fusion proteins (∼40kDa and ∼50kDa) were used to produce cocktail of PAb by immunizing rabbits, which simultaneously detected natural infection of CMV and PRSV or CMV, PRSV and GBNV in Cucurbitaceous, Solanaceous and other hosts in DAC-ELISA. This is the first report on production of a cocktail of PAb to recombinant fusion protein of two or three distinct viruses.

  5. Serodiagnostic potential of immuno-PCR using a cocktail of mycobacterial antigen 85B, ESAT-6 and cord factor in tuberculosis patients.

    Science.gov (United States)

    Singh, Netrapal; Sreenivas, Vishnubhatla; Sheoran, Abhishek; Sharma, Suman; Gupta, Krishna B; Khuller, Gopal K; Mehta, Promod K

    2016-01-01

    A novel indirect immuno-polymerase chain reaction (I-PCR) assay was developed for the detection of circulating anti-Ag85B (antigen 85B, Rv1886c), anti-ESAT-6 (early secretory antigenic target-6, Rv3875) and anti-cord factor (trehalose 6,6'-dimycolate) antibodies from the sera samples of pulmonary tuberculosis (PTB) and extrapulmonary tuberculosis (EPTB) patients and the results were compared with an analogous enzyme-linked immunosorbent assay (ELISA). We covalently attached the amino-modified reporter DNA to the dithiothreitol (DTT)-reduced anti-human IgG antibody through a chemical linker succinimidyl 4-[N-maleimidomethyl]-cyclohexane-1-carboxylate (SMCC). The detection of cocktail of anti-Ag85B, anti-ESAT-6 and anti-cord factor antibodies was found to be superior to the detection of individual antibodies. The sensitivities of 89.5% and 77.5% with I-PCR and 70.8% and 65% with ELISA were observed in smear-positive and smear-negative PTB cases, respectively with high specificity (90.9%). On the other hand, a sensitivity of 77.5% with I-PCR and 65% with ELISA was observed in EBTB cases. The detection of cocktail of antibodies by I-PCR is likely to improve the utility of existing algorithms for TB diagnosis.

  6. Two Party Pseudo Telepathy Games

    CERN Document Server

    Chakrabarty, I

    2006-01-01

    In this work we have introduced two party pseudo telepathy games with respective winning conditions. One cannot win these games deterministically by any kind of classical protocols. Interestingly we find out that in quantum world, these winning conditions can be achieved if the players share an entangled state. We also introduced a game which is impossible to win if the players are form classical world, (both probabilistically and deterministically) yet there exists a perfect quantum strategy by following which, one can attain the winning condition of the game.

  7. SynTec Final Technical Report: Synthetic biology for Tailored Enzyme cocktails

    Energy Technology Data Exchange (ETDEWEB)

    Lin, Janine [Novozymes, Inc., Davis, CA (United States); Teter, Sarah [Novozymes, Inc., Davis, CA (United States)

    2016-06-30

    Using a novel enzyme screening method inspired by synthetic biology, Novozymes developed new technology under SynTec which allows for more rapidly tailoring of enzyme cocktails. The methodology can be applied to specific feedstocks, and or coupled to address a specific hydrolytic conversion process context. Using combinatorial high throughput screening of libraries of enzyme domains, we can quickly assess which combination of catalytic modules delivers the best performance for a specific condition. To demonstrate the effectiveness of the screening process, we measured performance of the output catalytic cocktail compared to CTec3/HTec3. SynTec benchmark cocktail - blend of Cellic® CTec3 and HTec3. The test substrate was - ammonia fiber expansion pretreated corn stover (AFEX™ PCS).CTec3/HTec3 was assayed at the optimal pH and temperature, and also in the absence of any pH adjustment. The new enzyme cocktail discovered under SynTec was assayed in the absence of any pH adjustment and at the optimal temperature. Conversion is delivered by SynTec enzyme at significant dose reduction relative to CTec3/HTec3 at the controlled pH optimum, and without titrant required to maintain pH, which delivers additional cost savings relative to current state of the art process. In this 2.5 year $4M project, the team delivered an experimental cocktail that significantly outperformed CTec3/HTec3 for a specific substrate, and for specific hydrolysis conditions. As a means of comparing performance improvement delivered per research dollar spent, we note that SynTec delivered a similar performance improvement to the previous award, in a shorter time and with fewer resources than for the previously successful DOE project DECREASE, a 3.5 year, $25M project, though this project focused on a different substrate and used different hydrolysis conditions. The newly implemented technology for rapid sourcing of new cellulases and hemicellulases from nature is an example of Novozymes

  8. Political Parties between Conflict and Consensus, with Special Review on Party Finance: the Example of Germany

    Directory of Open Access Journals (Sweden)

    Hrvoje Matakovic

    2015-12-01

    Full Text Available Political parties as voluntary organizations of free citizens in democratic state feature their candidates for parliament and strive to gain the best results in elections. In order to participate in elections and accomplish the tasks they were founded for, political parties should have access to financial resources for paying expenditures related to their activities. This paper will show that political parties in Germany, contrary to the conflicts shown in public have build up mutual cooperation in developing generous system of party financing from public sources. Cooperation in political life and consensual decision making were developed in Germany as the outcome of the totalitarian past, and this conceptual background has been used by political parties in order to develop the system of public party financing. In this way party cartel was created, and parties took over the country and created party state.

  9. Selfish third parties act as peacemakers by transforming conflicts and promoting cooperation.

    Science.gov (United States)

    Halevy, Nir; Halali, Eliran

    2015-06-02

    The tremendous costs of conflict have made humans resourceful not only at warfare but also at peacemaking. Although third parties have acted as peacemakers since the dawn of history, little is known about voluntary, informal third-party intervention in conflict. Here we introduce the Peacemaker Game, a novel experimental paradigm, to model and study the interdependence between disputants and third parties in conflict. In the game, two disputants choose whether to cooperate or compete and a third party chooses whether or not to intervene in the conflict. Intervention introduces side payments that transform the game disputants are playing; it also introduces risk for the third party by making it vulnerable to disputants' choices. Six experiments revealed three robust effects: (i) The mere possibility of third-party intervention significantly increases cooperation in interpersonal and intergroup conflicts; (ii) reducing the risk to third parties dramatically increases intervention rates, to everyone's benefit; and (iii) disputants' cooperation rates are consistently higher than third parties' intervention rates. These findings explain why, how, and when self-interested third parties facilitate peaceful conflict resolution.

  10. Appointments and party organizations: network governmental party in kirchnerista peronism

    Directory of Open Access Journals (Sweden)

    Gerardo SCHERLIS

    2013-01-01

    Full Text Available This article contributes to the understanding of the organizational structures of peronism, Argentine dominant political force, on the basis of a study on partisan appointments. It presents the main findings of a research on the scope, the workings, the motivations, and the criteria of partisan appointments in the Argentine state, with particular attention to Néstor Kirchner administration (2003-2007. The more general argument contends that appointments became in this period a crucial tool to control the state apparatus while, at the same time, they made it possible to renovate Peronist partisan elites under the leadership of the president. The data, obtained from a large series of expert interviews, make it possible to offer a new theoretical framework for the understanding of party-government linkages in contemporary Argentine democracy.

  11. Activist Model of Political Party Growth

    CERN Document Server

    Jeffs, Rebecca A; Roach, Paul A; Wyburn, John

    2015-01-01

    The membership of British political parties has a direct influence on their political effectiveness. This paper applies the mathematics of epidemiology to the analysis of the growth and decline of such memberships. The party members are divided into activists and inactive members, where all activists influence the quality of party recruitment, but only a subset of activists recruit and thus govern numerical growth. The activists recruit for only a limited period, which acts as a restriction on further party growth. This Limited Activist model is applied to post-war and recent memberships of the Labour, Scottish National and Conservative parties. The model reproduces data trends, and relates realistically to historical narratives. It is concluded that the political parties analysed are not in danger of extinction but experience repeated periods of growth and decline in membership, albeit at lower numbers than in the past.

  12. Gender differences in Assessments of Party Leaders

    DEFF Research Database (Denmark)

    Kosiara-Pedersen, Karina; Hansen, Kasper Møller

    2015-01-01

    Is there a relationship between party leader gender and voters' assessments? Yes, according to theses on gender identity and stereotyping. A voter survey during the 2011 Danish general election allows for a comprehensive analysis of a less likely case with four male and four female party leaders....... Female party leaders are assessed more positively by female voters than male voters both in regard to general party leader sympathy and assessment of specific characteristics, whereas it is not the case that male party leaders are assessed more positively by male voters than female voters. The impact...... of gender does not increase with age, actually, the opposite is the case among men since younger male voters have smaller sympathy for female party leaders. Furthermore, there is no support for the expectation that voters with more education or with higher levels of political interest and knowledge are more...

  13. Gender differences in Assessments of Party Leaders

    DEFF Research Database (Denmark)

    Kosiara-Pedersen, Karina; Hansen, Kasper Møller

    2015-01-01

    Is there a relationship between party leader gender and voters' assessments? Yes, according to theses on gender identity and stereotyping. A voter survey during the 2011 Danish general election allows for a comprehensive analysis of a less likely case with four male and four female party leaders....... Female party leaders are assessed more positively by female voters than male voters both in regard to general party leader sympathy and assessment of specific characteristics, whereas it is not the case that male party leaders are assessed more positively by male voters than female voters. The impact...... of gender does not increase with age, actually, the opposite is the case among men since younger male voters have smaller sympathy for female party leaders. Furthermore, there is no support for the expectation that voters with more education or with higher levels of political interest and knowledge are more...

  14. Probiotic BIFICO cocktail ameliorates Helicobacter pylori induced gastritis.

    Science.gov (United States)

    Yu, Hong-Jing; Liu, Wei; Chang, Zhen; Shen, Hui; He, Li-Juan; Wang, Sha-Sha; Liu, Lu; Jiang, Yuan-Ying; Xu, Guo-Tong; An, Mao-Mao; Zhang, Jun-Dong

    2015-06-07

    To determine the protective effect of triple viable probiotics on gastritis induced by Helicobacter pylori (H. pylori) and elucidate the possible mechanisms of protection. Colonization of BIFICO strains in the mouse stomach was determined by counting colony-forming units per gram of stomach tissue. After treatment with or without BIFICO, inflammation and H. pylori colonization in the mouse stomach were analyzed by hematoxylin and eosin and Giemsa staining, respectively. Cytokine levels were determined by enzyme-linked immunosorbent assay and Milliplex. The activation of nuclear factor (NF)-κB and MAPK signaling in human gastric epithelial cells was evaluated by Western blot analysis. Quantitative reverse transcription-polymerase chain reaction was used to quantify TLR2, TLR4 and MyD88 mRNA expression in the mouse stomach. We demonstrated that BIFICO, which contains a mixture of Enterococcus faecalis, Bifidobacterium longum and Lactobacillus acidophilus, was tolerant to the mouse stomach environment and was able to survive both the 8-h and 3-d courses of administration. Although BIFICO treatment had no effect on the colonization of H. pylori in the mouse stomach, it ameliorated H. pylori-induced gastritis by significantly inhibiting the expression of cytokines and chemokines such as TNF-α, IL-1β, IL-10, IL-6, G-CSF and MIP-2 (P gastritis by inhibiting the inflammatory response in gastric epithelial cells.

  15. Formace hnutí Tea Party

    OpenAIRE

    Bicková, Eliška

    2014-01-01

    Tea Party affects American politics since 2009 when she began under the name of former revolutionary movement to fight government interventions with massive demonstrations. There was often incorrect expectation concerning Tea Party's potential in the first years of her activity. Tea Party supporters cannot be easily characterized, since a very differentiated group of Americans identifies itself with the movement, they come from a broad scale of social groups from Religious Right to libertaria...

  16. Book Review: Training the Party: Party Adaptation and Elite Training in Reform-era China

    DEFF Research Database (Denmark)

    Brødsgaard, Kjeld Erik

    2016-01-01

    Review of: Training the Party: Party Adaptation and Elite Training in Reform-era China. Charlotte P. Lee . Cambridge: Cambridge University Press, 2015. xii + 251 pp. $99.99. ISBN 978-1-107-09063-7......Review of: Training the Party: Party Adaptation and Elite Training in Reform-era China. Charlotte P. Lee . Cambridge: Cambridge University Press, 2015. xii + 251 pp. $99.99. ISBN 978-1-107-09063-7...

  17. Evaluation of a spasmolytic cocktail to prevent radial artery spasm during coronary procedures.

    Science.gov (United States)

    Kiemeneij, Ferdinand; Vajifdar, Bhavesh U; Eccleshall, Simon C; Laarman, GertJan; Slagboom, Ton; van der Wieken, Ron

    2003-03-01

    Radial artery spasm is a frequent complication of the transradial approach for coronary angiography and angioplasty. Recently, we have been able to quantify spasm using the automatic pullback device. The objective of this study was to assess the efficacy of an intra-arterial vasodilating cocktail in reducing the incidence and severity of radial artery spasm. A hundred patients undergoing coronary procedures via the radial artery were divided into two groups of 50 each. Patients in group A received intra-arterial cocktail (5 mg of verapamil plus 200 micro g nitroglycerine in 10 ml of normal saline), while patients in group B did not receive any vasodilating drug. The pullback device was used for sheath removal at the end of the procedure. Seven (14%) patients in group A experienced pain (i.e., pain score of III-V) during automatic sheath removal, as compared to 17 (34%) in group B (P = 0.019). The mean pain score was significantly lower in group A than in group B (1.7 +/- 0.94 vs. 2.08 +/- 1.07; P = 0.03). The maximal pullback force (MPF) was also significantly lower for group A (0.53 +/- 0.52 kg; range, 0.10-3.03 kg) as compared to group B (0.76 +/- 0.45 kg; range, 0.24-1.99 kg; P = 0.013). Only 4 (8%) patients in group A had an MPF value greater than 1.0 kg, suggesting the presence of significant radial artery spasm, as opposed to 11 (22%) in group B (P = 0.029). Administration of an intra-arterial vasodilating cocktail prior to sheath insertion reduces the occurrence and severity of radial artery spasm.

  18. Party media agenda-setting: how parties influence election news coverage

    NARCIS (Netherlands)

    D.N. Hopmann; C. Elmelund-Præstekær; E. Albæk; R. Vliegenthart; C.H. de Vreese

    2012-01-01

    Political parties have substantial influence on which issues the news media cover during election campaigns, while the media have limited influence on party agendas. However, we know little about why some parties are more successful than others in passing the media’s gates and being covered on spons

  19. Modeling Response to Advertising and Pricing Changes for “V-8” Cocktail Vegetable Juice

    OpenAIRE

    Joseph O. Eastlack, Jr.; Ambar G. Rao

    1986-01-01

    This paper is based on a series of studies undertaken for “V-8” Cocktail Vegetable Juice over a five-year period. The studies were a consequence of management questions regarding the effectiveness of a new advertising campaign, the best media mix for this campaign and the apparent “wear-out” of the advertising copy. The studies included controlled experimentation, estimation of advertising response functions, and exploration of price sensitivity following major price increases by “V-8.” It wa...

  20. Variations in the usage and composition of a radial cocktail during radial access coronary angiography procedures.

    LENUS (Irish Health Repository)

    Pate, G

    2011-10-01

    A survey was conducted of medication administered during radial artery cannulation for coronary angiography in 2009 in Ireland; responses were obtained for 15 of 20 centres, in 5 of which no radial access procedures were undertaken. All 10 (100%) centres which provided data used heparin and one or more anti-spasmodics; verapamil in 9 (90%), nitrate in 1 (10%), both in 2 (20%). There were significant variations in the doses used. Further work needs to be done to determine the optimum cocktail to prevent radial artery injury following coronary angiography.

  1. 11 CFR 9004.1 - Major parties.

    Science.gov (United States)

    2010-01-01

    ... Consumer Price Index in the manner described in 11 CFR 110.17(a). ... ENTITLEMENT OF ELIGIBLE CANDIDATES TO PAYMENTS; USE OF PAYMENTS § 9004.1 Major parties. The eligible candidates of each major party in a Presidential election shall be entitled to equal payments under 11...

  2. Sex Parties: Female Teen Sexual Experimentation

    Science.gov (United States)

    Toscano, Sharyl Eve

    2006-01-01

    Adolescent participants in a study aimed at exploring the nature and characteristics of girls' dating relationships revealed the phenomenon of sex parties. These teens defined a "sex party" as an opportunity to engage in sexual contact outside of typical dating relationships. Sexual activity could involve actual intercourse, but usually involved…

  3. Rural protest groups and populist political parties

    NARCIS (Netherlands)

    Strijker, Derk; Voerman, Gerrit; Terluin, Ida

    2015-01-01

    Some agricultural or rural protest groups in the Western world evolved into political parties - often of a populist nature - whereas others did not. This book is the first to explore under which conditions this happens, and to what extent current populist parties have agricultural or rural ties and

  4. Crossing boundaries : Involving external parties in innovation

    NARCIS (Netherlands)

    Slot, J.H.

    2013-01-01

    To improve the return on investments in innovation, firms increasingly open up their new product development (NPD) processes by inviting external parties to participate. This dissertation focuses on the involvement of three different types of external parties in the NPD process: suppliers, customers

  5. Religion and party choice in Europe

    NARCIS (Netherlands)

    van der Brug, W.; Hobolt, S.B.; de Vreese, C.H.

    2009-01-01

    This paper investigates religiosity in relation to party choice in European Parliament elections. Conventional wisdom tells us that as Europe has secularised, the effect of religion on party choice should also have diminished. Yet, this cross-national and cross-temporal study of religious voting in

  6. Rural protest groups and populist political parties

    NARCIS (Netherlands)

    Strijker, Derk; Voerman, Gerrit; Terluin, Ida

    2015-01-01

    Some agricultural or rural protest groups in the Western world evolved into political parties - often of a populist nature - whereas others did not. This book is the first to explore under which conditions this happens, and to what extent current populist parties have agricultural or rural ties and

  7. Pristine Purity : New Political Parties in Canada

    NARCIS (Netherlands)

    Lucardie, Anthonie

    2007-01-01

    Success sells better than failure; hence new parties receive very little attention from political scientists as long as they remain marginal and fail to win seats in Parliament. Yet in the margins of the party system, they may maintain the pristine purity of political principles and ideas better tha

  8. Party Governance and the Selection of Parliamentarians

    NARCIS (Netherlands)

    K.J. Beniers (Klaas)

    2005-01-01

    textabstractThis paper examines the incentives for a party leader in office and for a parties' rank-and-file to replace a sitting member of parliament. As to the leader's decision, we show that the leader prefers to replace a critical member of parliament who votes against the leader's policy. A com

  9. 7 CFR 29.28 - Interested party.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Interested party. 29.28 Section 29.28 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... INSPECTION Regulations Definitions § 29.28 Interested party. The owner or other financially interested...

  10. Independent Local Political Parties in the Netherlands

    NARCIS (Netherlands)

    Voerman, G.; Boogers, M.

    2010-01-01

    In the last 15 years, the Netherlands has witnessed the enormous growth of independent local parties and of their electoral support. In order to assess the success of independent locals in the Netherlands, this paper explores how independent local parties distinguish themselves ideologically, organi

  11. Leakage Resilient Secure Two-Party Computation

    DEFF Research Database (Denmark)

    Damgård, Ivan Bjerre; Hazay, Carmit; Patra, Arpita

    2012-01-01

    Definitions.} We formalize the notion of secure two-party computation in the presence of leakage and introduce security definitions in the {\\em ideal/real framework}. Our formalization induces two types of adversarial attacks. We further study the feasibility of our definitions in the computational setting......In the traditional {\\em secure function evaluation} setting, some set of distrusting parties jointly compute a function of their respective inputs {\\em securely} as if the computation is executed in an ideal setting where the parties send inputs to a trusted party that performs the computation...... and explore some of the conditions under which these definitions are met. \\item {\\em Composition Theorems.} We provide compositions theorems for our new modelings. Our results provide compositions theorems for the case where the inputs of the parties are sampled from a min-entropy source distribution. \\item...

  12. The edible cocktail: the effect of sugar and alcohol impregnation on the crunchiness of fruit

    Directory of Open Access Journals (Sweden)

    Scholten Elke

    2012-06-01

    Full Text Available Abstract Background Vacuum impregnation is seen as a valuable technique for flavor pairing in the catering industry. One of the applications of this technique is the creation of edible cocktails by impregnating fruits with liquors, leading to an interplay of different flavors. However, the effect of the impregnation of sugar and alcohol into the fruit will affect the texture of the fruit and therefore its crunchiness. Thus, the positive effect of flavor pairing might be inhibited by a negative effect in texture changes. Results This investigation focused on the change in crunchiness as a result of the impregnation of different sugar and alcohol containing solutions. When hypotonic solutions were used, the impregnation resulted in the rupture of the cells, thereby leading to a decrease in crunchiness. When hypertonic solutions were used, the cells shrunk, which also resulted in a decrease in crunchiness. Isotonic solutions resulted in crunchiness comparable to its fresh version. When alcohol was used, the crunchiness decreased at all concentrations investigated. Conclusions Crunchiness of fruit can only be maintained when impregnated with isotonic sugar solutions. When the sugar or alcohol content deviates from that in the fruit, impregnation of these liquids will lead to a decrease in crunchiness. This has consequences for the creation of edible cocktails: for an optimal crunchiness, the sugar content of the impregnation liquid has to be equal to the sugar content of the fruit or vegetable.

  13. Neuroprotection by Cocktails of Dietary Antioxidants under Conditions of Nerve Growth Factor Deprivation.

    Science.gov (United States)

    Amara, Flavio; Berbenni, Miluscia; Fragni, Martina; Leoni, Giampaolo; Viggiani, Sandra; Ippolito, Vita Maria; Larocca, Marilena; Rossano, Rocco; Alberghina, Lilia; Riccio, Paolo; Colangelo, Anna Maria

    2015-01-01

    Dietary antioxidants may be useful in counteracting the chronic inflammatory status in neurodegenerative diseases by reducing oxidative stress due to accumulation of reactive oxygen species (ROS). In this study, we newly described the efficacy of a number of dietary antioxidants (polyphenols, carotenoids, thiolic compounds, and oligoelements) on viability of neuronal PC12 cells following Nerve Growth Factor (NGF) deprivation, a model of age-related decrease of neurotrophic support that triggers neuronal loss. Neuroprotection by antioxidants during NGF deprivation for 24 h was largely dependent on their concentrations: all dietary antioxidants were able to efficiently support cell viability by reducing ROS levels and restoring mitochondrial function, while preserving the neuronal morphology. Moreover, ROS reduction and neuroprotection during NGF withdrawal were also achieved with defined cocktails of 3-6 different antioxidants at concentrations 5-60 times lower than those used in single treatments, suggesting that their antioxidant activity was preserved also at very low concentrations. Overall, these data indicate the beneficial effects of antioxidants against oxidative stress induced by decreased NGF availability and suggest that defined cocktails of dietary factors at low concentrations might be a suitable strategy to reduce oxidative damage in neurodegenerative diseases, while limiting possible side effects.

  14. Regulation of adipogenesis by medium-chain fatty acids in the absence of hormonal cocktail.

    Science.gov (United States)

    Yang, Jeong-Yeh; Della-Fera, Mary Anne; Rayalam, Srujana; Park, Hea Jin; Ambati, Suresh; Hausman, Dorothy B; Hartzell, Diane L; Baile, Clifton A

    2009-07-01

    We report here that octanoate and decanoate, 8-carbon and 10-carbon medium-chain fatty acids (MCFA), decreased adipogenesis in 3T3-L1 preadipocytes when treated with standard hormonal cocktail, but increased adipogenesis in a dose-dependent manner (with decanoate being more effective) when treated with basal media. Addition of dexamethasone to basal medium with either octanoate or decanoate further increased adipogenesis. In order to understand the adipogenic effects of MCFA in the absence of standard hormonal cocktail, postconfluent 3T3-L1 preadipocytes were treated with octanoate or decanoate, and the change in the expression of several adipogenic transcription factors and enzymes was investigated using real-time RT-PCR. Octanoate and decanoate up-regulated the mRNA expression of peroxisome-proliferator-activated receptor (PPAR) gamma, CCAAT/enhancer-binding protein (C/EBP) alpha, fatty-acid-binding protein, sterol-regulatory element binding protein 1c, lipoprotein lipase and hormone-sensitive lipase, and the protein expression of PPARgamma and C/EBPalpha, with decanoate being more effective. Moreover, the PPARgamma antagonist GW9662 inhibited MCFA-induced lipid accumulation by about 50%. Decanoate and octanoate, to a lesser degree, increased lipid accumulation, which was associated with an increase in glycerol-3-phosphate dehydrogenase activity. These results show that octanoate and decanoate may stimulate differentiation of preadipocytes, at least in part, by their influence on the expression of PPARgamma and other adipocyte-specific factors.

  15. Neuroprotection by Cocktails of Dietary Antioxidants under Conditions of Nerve Growth Factor Deprivation

    Science.gov (United States)

    Amara, Flavio; Berbenni, Miluscia; Fragni, Martina; Leoni, Giampaolo; Viggiani, Sandra; Ippolito, Vita Maria; Larocca, Marilena; Rossano, Rocco; Alberghina, Lilia; Riccio, Paolo; Colangelo, Anna Maria

    2015-01-01

    Dietary antioxidants may be useful in counteracting the chronic inflammatory status in neurodegenerative diseases by reducing oxidative stress due to accumulation of reactive oxygen species (ROS). In this study, we newly described the efficacy of a number of dietary antioxidants (polyphenols, carotenoids, thiolic compounds, and oligoelements) on viability of neuronal PC12 cells following Nerve Growth Factor (NGF) deprivation, a model of age-related decrease of neurotrophic support that triggers neuronal loss. Neuroprotection by antioxidants during NGF deprivation for 24 h was largely dependent on their concentrations: all dietary antioxidants were able to efficiently support cell viability by reducing ROS levels and restoring mitochondrial function, while preserving the neuronal morphology. Moreover, ROS reduction and neuroprotection during NGF withdrawal were also achieved with defined cocktails of 3–6 different antioxidants at concentrations 5–60 times lower than those used in single treatments, suggesting that their antioxidant activity was preserved also at very low concentrations. Overall, these data indicate the beneficial effects of antioxidants against oxidative stress induced by decreased NGF availability and suggest that defined cocktails of dietary factors at low concentrations might be a suitable strategy to reduce oxidative damage in neurodegenerative diseases, while limiting possible side effects. PMID:26236423

  16. Defined enzyme cocktail from the anaerobic fungus Orpinomyces sp. strain C1A effectively releases sugars from pretreated corn stover and switchgrass

    Science.gov (United States)

    Morrison, Jessica M.; Elshahed, Mostafa S.; Youssef, Noha H.

    2016-01-01

    The anaerobic fungus Orpinomyces strain C1A is capable of growth on various types of lignocellulosic substrates, and harbors an impressive reservoir of carbohydrate active enzymes (CAZymes). Using a minimum enzyme cocktail strategy, we constituted a four-component lignocellulolytic cocktail derived from highly transcribed C1A, and evaluated its efficacy against pretreated corn stover and switchgrass. Hydrolysis yields ranged between 65–77.4%, depending on the lignocellulosic substrate and pretreatment applied. Addition of a highly expressed anaerobic fungal swollenin improved hydrolysis yields by up to 7%. Compared to the commercial cocktail CTec2, these anaerobic fungal cocktails provided comparable or slightly lower hydrolysis yields. Further, the differences in efficacy between commercial and anaerobic cocktails were often only realized after extended (168 hr) incubations. Under certain conditions, the hydrolysis yields of the anaerobic fungal cocktail was slightly superior to that realized by CTec2. We attribute the observed high hydrolysis yields to the high specific activity and affinity of the individual enzymes of the cocktail, as well as the high level of synergy and multi-functionality observed in multiple components. Collectively, this effort provides a novel platform for constructing highly effective enzymes for biofuel production and represents the first lignocellulolytic enzyme cocktail created from anaerobic fungal enzymes. PMID:27381262

  17. Political Marketing Mix in Indonesia Parties

    Directory of Open Access Journals (Sweden)

    Freddy Simbolon

    2016-05-01

    Full Text Available The reform era has changed the paradigm of Indonesian politics. Multiparty system has demanded a political party to be able to compete for their constituents. Article aimed to convey a political marketing concept that can helppolitical parties in planning a strategy to win the election. Research method used descriptive method analysis. The results suggest that the approach of the marketing mix that includes the product, price, place, and promotion areapproaches that are very helpful in presenting a plan to win the marketing strategy of political parties in elections.

  18. The Labour Party and British Republicanism

    Directory of Open Access Journals (Sweden)

    Kenneth O. MORGAN

    2003-10-01

    Full Text Available The famous detective, Sherlock Holmes, once solved a case by referring to “the dog that did not bark.” In the past 250 years of British history, republicanism is another dog that did not bark. This is particularly true of supposedly our most radical major political party, the Labour Party. Over the monarchy, as over constitutional matters generally, Labour’s instincts have been conservative. Even after 1997, when the party, led by Lord Irvine, has indeed embarked upon major constitutional ref...

  19. Bacteriophage cocktail and multi-strain probiotics in the feed for weanling pigs: effects on intestine morphology and targeted intestinal coliforms and Clostridium.

    Science.gov (United States)

    Kim, J S; Hosseindoust, A; Lee, S H; Choi, Y H; Kim, M J; Lee, J H; Kwon, I K; Chae, B J

    2017-01-01

    Two experiments were conducted to investigate the effects of dietary supplementation of bacteriophage cocktail, probiotics and a combination of these two supplements on performance and gut health of weanling pigs. In Experiment 1, 150 weaned piglets were randomly allotted to three treatments on the basis of BW. The dietary treatments included a basal diet supplemented with 0 (control), 1.0 and 1.5 g/kg bacteriophage cocktail. Pigs fed 1.0 and 1.5 g/kg bacteriophage product had greater (Pprobiotic product (P), 1.0 g/kg bacteriophage cocktail (B) and combination of 1.0 g/kg bacteriophage cocktail and 3.0 g/kg fermented probiotic product. Pigs fed bacteriophage cocktail diets had greater (PProbiotics significantly increased G : F, colonization of Lactobacillus spp. in ileum. At day 35, bacteriophage treatment group showed greater (Pgut health of weanling pigs, however their combination with probiotics did not show an interaction.

  20. In vitro cytochrome P450 inhibition potential of methylenedioxy-derived designer drugs studied with a two-cocktail approach.

    Science.gov (United States)

    Dinger, Julia; Meyer, Markus R; Maurer, Hans H

    2016-02-01

    In vitro cytochrome P450 (CYP) inhibition assays are common approaches for testing the inhibition potential of drugs for predicting potential interactions. In contrast to marketed medicaments, drugs of abuse, particularly the so-called novel psychoactive substances, were not tested before distribution and consumption. Therefore, the inhibition potential of methylenedioxy-derived designer drugs (MDD) of different drug classes such as aminoindanes, amphetamines, benzofurans, cathinones, piperazines, pyrrolidinophenones, and tryptamines should be elucidated. The FDA-preferred test substrates, split in two cocktails, were incubated with pooled human liver microsomes and analysed after protein precipitation using LC-high-resolution-MS/MS. IC50 values were determined of MDD showing more than 50 % inhibition in the prescreening. Values were calculated by plotting the relative metabolite concentration formed over the logarithm of the inhibitor concentration. All MDD showed inhibition against CYP2D6 activity and most of them in the range of the clinically relevant CYP2D6 inhibitors quinidine and fluoxetine. In addition, the beta-keto compounds showed inhibition of the activity of CYP2B6, 5,6-MD-DALT of CYP1A2 and CYP3A, and MDAI of CYP2A6, all in the range of clinically relevant inhibitors. In summary, all MDD showed inhibition of the activity of CYP2D6, six of CYP1A2, three of CYP2A6, 13 of CYP2B6, two of CYP2C9, six of CYP2C19, one of CYP2E1, and six of CYP3A. These results showed that the CYP inhibition by MDD might be clinically relevant, but further studies are needed for final conclusions.

  1. A binding hotspot in Trypanosoma cruzi histidyl-tRNA synthetase revealed by fragment-based crystallographic cocktail screens.

    Science.gov (United States)

    Koh, Cho Yeow; Siddaramaiah, Latha Kallur; Ranade, Ranae M; Nguyen, Jasmine; Jian, Tengyue; Zhang, Zhongsheng; Gillespie, J Robert; Buckner, Frederick S; Verlinde, Christophe L M J; Fan, Erkang; Hol, Wim G J

    2015-08-01

    American trypanosomiasis, commonly known as Chagas disease, is a neglected tropical disease caused by the protozoan parasite Trypanosoma cruzi. The chronic form of the infection often causes debilitating morbidity and mortality. However, the current treatment for the disease is typically inadequate owing to drug toxicity and poor efficacy, necessitating a continual effort to discover and develop new antiparasitic therapeutic agents. The structure of T. cruzi histidyl-tRNA synthetase (HisRS), a validated drug target, has previously been reported. Based on this structure and those of human cytosolic HisRS, opportunities for the development of specific inhibitors were identified. Here, efforts are reported to identify small molecules that bind to T. cruzi HisRS through fragment-based crystallographic screening in order to arrive at chemical starting points for the development of specific inhibitors. T. cruzi HisRS was soaked into 68 different cocktails from the Medical Structural Genomics of Pathogenic Protozoa (MSGPP) fragment library and diffraction data were collected to identify bound fragments after soaking. A total of 15 fragments were identified, all bound to the same site on the protein, revealing a fragment-binding hotspot adjacent to the ATP-binding pocket. On the basis of the initial hits, the design of reactive fragments targeting the hotspot which would be simultaneously covalently linked to a cysteine residue present only in trypanosomatid HisRS was initiated. Inhibition of T. cruzi HisRS was observed with the resultant reactive fragments and the anticipated binding mode was confirmed crystallographically. These results form a platform for the development of future generations of selective inhibitors for trypanosomatid HisRS.

  2. PEASANTRY, FAMILY PARTIES AND MEANINGS OF KINSHIP

    Directory of Open Access Journals (Sweden)

    Josiane Carine Wedig

    2013-12-01

    Full Text Available The authors focus the study on the Family Party taken as a privileged space to grasp symbolic categories departing from which the peasants build kinship. These parties bring to the foreground kinship meanings departing from the revival of a myth of origin and the affirmation of broad kinship binds. The study is based on an ethnographical research conducted within a Family Party of a rural community of German immigrants’ descendents, located in Rio Grande do Sul, Brazil. Research findings led the authors to consider that the party participants establish different meanings for kinship, which gather people that either descend from a common ancestral or have a connection with him or her, thus constituting a relation of familial or ethnic belonging.

  3. Efficacy of multimodal cocktail periarticular injection with or without steroid in total knee arthroplasty

    Institute of Scientific and Technical Information of China (English)

    YUE De-bo; WANG Bai-liang; LIU Kun-peng; GUO Wan-shou

    2013-01-01

    Background Multimodal cocktail periarticular injection (MCPI) with a large volume of low concentration local anesthetics,adrenaline,and anti-inflammatory agents such as non-steroidal anti-inflammatory drug or steroids have shown good pain control and improvement in range of motion after surgery.This study compares the efficacy of pain control after total knee arthroplasty,using multimodal cocktail periarticular injection with steroid or without steroid.Methods This is a prospective,double-blinded,randomized and control study.Seventy-two patients with osteoarthritis that met clinical criteria for total knee arthroplasty were recruited into the study,and were randomized to receive either multimodal cocktail periarticular injection with steroid or without steroid.Pain was assessed by visual analogue scale (VAS) at preoperative and postoperative at rest,and during activity.The range of motion was recorded preoperatively and postoperatively.The amount of daily and cumulative morphine consumption were measured by patient-controlled analgesia in the first 72 hours postoperatively.The duration of celecoxib usage was also recorded at the last follow-up.Results There were no differences between the non-steroid and steroid groups with regard to VAS at rest and during activity,or range of motion,at any postoperative observation time.The postoperative Knee Society Knee Score in the steroid group improved significantly as compared with that in non-steroid group at the one-month (84.1±13.1 and 65.9±12.1; P <0.0045),three-month follow-up (90.2±16.3 and 72.5±16.6; P <0.0027),but after postoperative six-month the Knee Society Knee Score showed no significant difference between the groups.There was no significant difference in consumption of the morphine about daily or total consumption within 72 hours between the two groups.The duration of celecoxib usage in patients in the steroid group was significantly shorter than that in the non-steroid group ((7.2±0.7) compared with

  4. Political Parties and Democracy in Haiti

    Science.gov (United States)

    2001-06-01

    States continues its xv efforts to hold Haitian elites accountable to the ideals and rules of their democratic system in order for party system...schism between the ruling party and a democratic alternative that seeks political power outside of Haiti�s legitimate democratic system . Such actions...States should continue in its efforts to hold Haitian elites accountable to the ideals and rules of a democratic system . The repressive and corrupt

  5. Movements in Parties: OccupyPD

    Directory of Open Access Journals (Sweden)

    Donatella della Porta

    2015-03-01

    Full Text Available When the United States activists called for people to Occupy#everywhere, it is unlikely they were thinking of the headquarters of the Italian centre-left party. Parties and movements are often considered to be worlds apart. In reality, parties have been relevant players in movement politics, and movements have influenced parties, often through the double militancy of many of their members. OccupyPD testifies to a continuous fluidity at the movement-party border, but also to a blockage in the party’s interactions with society that started long before the economic crisis but drastically accelerated with it. In this paper we present the OccupyPD Movement as a case of interaction between party politics and social movement politics, and in particular between the base membership of a centre-left party and the broader anti-austerity movement that diffused from the US to Europe adopting similar forms of actions and claims. Second, by locating it within the context of the economic and democratic crisis that erupted in 2007, we understand its emergence as a reaction towards politics in times of crisis of responsibility, by which we mean a drastic drop in the capacity of the government to respond to citizens’ requests. To fulfil this double aim, we bridge social movement studies with research on party change, institutional trust and democratic theory, looking at some political effects of the economic crisis in terms of a specific form of legitimacy crisis, as well as citizens’ responses to it, with a particular focus on the political meaning of recent anti-austerity protests. In this analysis, we refer to both quantitative and qualitative data from secondary liter-ature and original in-depth interviews carried out with a sample of OccupyPD activists.

  6. Third Party Involvement in Barroom Conflicts

    OpenAIRE

    Parks, Michael J.; Osgood, D. Wayne; Richard B. Felson; Wells, Samantha; Graham, Kathryn

    2013-01-01

    This study examines the effect of situational variables on whether third parties intervene in conflicts in barroom settings, and whether they are aggressive or not when they intervene. Based on research on bystander intervention in emergencies, we hypothesized that third parties would be most likely to become involved in incidents with features that convey greater danger of serious harm. The situational variables indicative of danger were severity of aggression, whether the aggression was one...

  7. Party Formation in the United States

    OpenAIRE

    2013-01-01

    This dissertation is about how political parties formed in the world's first mass democracy, the United States. I trace the process of party formation from the bottom up. First, I ask: How do individuals become engaged in politics and develop political affiliations? In most states, throughout the antebellum era, the county was the primary unit of political administration and electoral representation. Owing to their small size, contiguity, and economic homogeneity, I expect that each county's ...

  8. Motivated Reasoning and Political Parties: Evidence for Increased Processing in the Face of Party Cues

    DEFF Research Database (Denmark)

    Petersen, Michael Bang; Skov, Martin; Serritzlew, Søren;

    2013-01-01

    Extant research in political science has demonstrated that citizens’ opinions on policies are influenced by their attachment to the party sponsoring them. At the same time, little evidence exists illuminating the psychological processes through which such party cues are filtered. From...... the psychological literature on source cues, we derive two possible hypotheses: (1) party cues activate heuristic processing aimed at minimizing the processing effort during opinion formation, and (2) party cues activate group motivational processes that compel citizens to support the position of their party....... As part of the latter processes, the presence of party cues would make individuals engage in effortful motivated reasoning to produce arguments for the correctness of their party’s position. Following psychological research, we use response latency to measure processing effort and, in support...

  9. Cocktail-Dosing Microdialysis Study to Simultaneously Assess Delivery of Multiple Organic-Cationic Drugs to the Brain.

    Science.gov (United States)

    Kitamura, Atsushi; Okura, Takashi; Higuchi, Kei; Deguchi, Yoshiharu

    2016-02-01

    Brain microdialysis is a powerful tool to estimate brain-to-plasma unbound concentration ratio at the steady state (Kp,uu) of compounds by direct measurement of the unbound concentration in brain interstitial fluid. Here, we evaluated a method to estimate Kp,uu values of multiple organic-cationic drugs simultaneously, by means of brain microdialysis combined with cocktail dosing. Five cationic drugs (diphenhydramine, memantine, oxycodone, pyrilamine, and tramadol), substrates of the proton-coupled organic cation antiport system, were selected as model drugs, and compared under single-dosing and cocktail-dosing conditions. We selected doses of the drugs at which no significant drug-drug interaction occurs at the proton-coupled organic cation antiport system in the blood-brain barrier (BBB). This was confirmed by uptake studies in hCMEC/D3 cells, an in vitro BBB model. The Kp,uu values after cocktail administration were in the range of 1.8-5.2, and were in good agreement with those after single administration. These results suggest that the microdialysis method with cocktail dosing is suitable to estimate Kp,uu values of several cationic drugs simultaneously, if there is no drug-drug interaction during BBB transport. The method could be useful for evaluating drug candidates with high Kp,uu values at an early stage in the development of central nervous system-acting drugs.

  10. Coverage of diarrhoea-associated Escherichia coli isolates from different origins with two types of phage cocktails

    Science.gov (United States)

    Bourdin, Gilles; Navarro, Armando; Sarker, Shafiqul A; Pittet, Anne-C; Qadri, Firdausi; Sultana, Shamima; Cravioto, Alejandro; Talukder, Kaisar A; Reuteler, Gloria; Brüssow, Harald

    2014-01-01

    Eighty-nine T4-like phages from our phage collection were tested against four collections of childhood diarrhoea-associated Escherichia coli isolates representing different geographical origins (Mexico versus Bangladesh), serotypes (69 O, 27 H serotypes), pathotypes (ETEC, EPEC, EIEC, EAEC, VTEC, Shigella), epidemiological settings (community and hospitalized diarrhoea) and years of isolation. With a cocktail consisting of 3 to 14 T4-like phages, we achieved 54% to 69% coverage against predominantly EPEC isolates from Mexico, 30% to 53% against mostly ETEC isolates from a prospective survey in Bangladesh, 24% to 61% against a mixture of pathotypes isolated from hospitalized children in Bangladesh, and 60% coverage against Shigella isolates. In comparison a commercial Russian phage cocktail containing a complex mixture of many different genera of coliphages showed 19%, 33%, 50% and 90% coverage, respectively, against the four above-mentioned collections. Few O serotype-specific phages and no broad-host range phages were detected in our T4-like phage collection. Interference phenomena between the phage isolates were observed when constituting larger phage cocktails. Since the coverage of a given T4-like phage cocktail differed with geographical area and epidemiological setting, a phage composition adapted to a local situation is needed for phage therapy approaches against E. coli pathogens. PMID:24528873

  11. Evaluation of the "Kosish Cocktail" in treating severe pain in "Home Care" in Morphine-naïve communities

    Directory of Open Access Journals (Sweden)

    A K Dam

    2010-01-01

    Conclusions: On the basis of this qualitative study, the authors confirm the efficacy and safety of the use of the Kosish cocktail in treating severe pain, and strongly recommend it for newly started hospices, especially for use in the domiciliary set-up.

  12. Party cohesion in the Icelandic Althingi

    Directory of Open Access Journals (Sweden)

    Gunnar Helgi Kristinsson

    2011-12-01

    Full Text Available Institutional theories of party cohesion may be divided into "nomination theories" and "structure of the executive theories". The former seek explanations of cohesion in the way nominations are conducted, predicting that de-centralized and inclusive nominations will reduce party cohesion. The latter attempt to explain cohesion by reference to the structure of the executive, and predict that parliamentary government will increase cohesion. Party cohesion in the Icelandic Althingi has, hitherto, not been extensively studied. In this article, large amounts of data are explored to test hypotheses derived from the two theoretical approaches. The analysis is based on roll-call data dating back to 1961 and electronic voting records from 1991 onwards. The main conclusion is that party cohesion is at a high level in Iceland, despite decentralized and inclusive nominations, and hypotheses derived from nomination theories therefore find no support in our data. Hypotheses derived from "structure of the executive theories" fare much better and the main reason for high party cohesion in Iceland seems to be parliamentary government. Various features of our data, however, encourage us not to ignore other contextual features affecting party cohesion, which neither of the two institutional theories can account for satisfactorily.

  13. Third party involvement in barroom conflicts.

    Science.gov (United States)

    Parks, Michael J; Osgood, D Wayne; Felson, Richard B; Wells, Samantha; Graham, Kathryn

    2013-01-01

    This study examines the effect of situational variables on whether third parties intervene in conflicts in barroom settings, and whether they are aggressive or not when they intervene. Based on research on bystander intervention in emergencies, we hypothesized that third parties would be most likely to become involved in incidents with features that convey greater danger of serious harm. The situational variables indicative of danger were severity of aggression, whether the aggression was one-sided or mutual, gender, and level of intoxication of the initial participants in the conflict. Analyses consist of cross-tabulations and three-level Hierarchical Logistic Models (with bar, evening, and incidents as levels) for 860 incidents of verbal and physical aggression from 503 nights of observation in 87 large bars and clubs in Toronto, Canada. Third party involvement was more likely during incidents in which: (1) the aggression was more severe; (2) the aggression was mutual (vs. one-sided) aggression; (3) only males (vs. mixed gender) were involved; and (4) participants were more intoxicated. These incident characteristics were stronger predictors of non-aggressive third party involvement than aggressive third party involvement. The findings suggest that third parties are indeed responding to the perceived danger of serious harm. Improving our knowledge about this aspect of aggressive incidents is valuable for developing prevention and intervention approaches designed to reduce aggression in bars and other locations. © 2013 Wiley Periodicals, Inc.

  14. RECHERCHES SUR LE DEVELOPPEMENT DES COCKTAILS AVEC UN POTENTIEL ANTIOXYDANT ELEVE

    Directory of Open Access Journals (Sweden)

    Daniela Istrati

    2010-12-01

    Full Text Available The research aimed to study the influence of the extraction process on the content of antioxidant compounds present in four types of tea and establishing of the optimal conditions for preparation of cocktails in whose composition is combined the beneficial effect of foods rich in antioxidants and vitamins: green tea, exotic fruits, berries and honey. Content of antioxidants, protein, fiber, vitamins and minerals from unfermented tea is supplemented by high concentration of biological active compounds in fruits and honey used. A positive correlation between substances added with significant increase of antioxidant and nutritive potential has been observed. This study confirms that the synergistic action of green tea, fruits and honey allowed obtaining attractive drinks from sensorial point of view and compounds with high antioxidant potential content.

  15. Improvement on sugar cane bagasse hydrolysis using enzymatic mixture designed cocktail.

    Science.gov (United States)

    Bussamra, Bianca Consorti; Freitas, Sindelia; Costa, Aline Carvalho da

    2015-01-01

    The aim of this work was to study cocktail supplementation for sugar cane bagasse hydrolysis, where the enzymes were provided from both commercial source and microorganism cultivation (Trichoderma reesei and genetically modified Escherichia coli), followed by purification. Experimental simplex lattice mixture design was performed to optimize the enzymatic proportion. The response was evaluated through hydrolysis microassays validated here. The optimized enzyme mixture, comprised of T. reesei fraction (80%), endoglucanase (10%) and β-glucosidase (10%), converted, theoretically, 72% of cellulose present in hydrothermally pretreated bagasse, whereas commercial Celluclast 1.5L converts 49.11%±0.49. Thus, a rational enzyme mixture designed by using synergism concept and statistical analysis was capable of improving biomass saccharification.

  16. Frankincense derived heavy terpene cocktail boosting breast cancer cell(MDA-MB-231) death in vitro简

    Institute of Scientific and Technical Information of China (English)

    Faruck; Lukmanul; Hakkim; Mohammed; Al-Buloshi; Jamal; Al-Sabahi

    2015-01-01

    Objective: To investigate the anti-cancer effect of frankincense derived heavy oil obtained by Soxhlet extraction method on breast cancer cells(MDA-MB-231), and to study its chemical profile using gas chromatography mass spectrometry analysis.Methods: Hexane was used to extract heavy oil from frankincense resin. Chemical profiling of heavy oil was done using Perkin Elmer Clarus GC system with mass spectrometer. MDA-MB-231 cells were treated with different dilutions(1:1 000, 1:1 500,1:1 750, 1:2 000, 1:2 250, 1:2 500, 1:2 750, 1:3 000, 1:3 250) of heavy oil for 24 h. The cells were observed by using light microscopy. Cell viability was measured by MTT assay.Results: Gas chromatography mass spectrometry chemical profiling of frankincense derived heavy oil revealed the presence of terpenes such as a-pinene(61.56%), a-amyrin(20.6%), b-amyrin(8.1%), b-phellandrene(1.47%) and camphene(1.04%). Heavy terpene cocktail induced significant MDA-MB-231 cell death at each concentration tested. Noticeably, very low concentration of Soxhlet derived heavy terpenes elicits considerable cytotoxicity on MDA-MB-231 cells compared to hydro distillated essential oil derived from frankincense resin.Conclusions: Extracting anti-cancer active principle cocktail by simple Soxhlet method is cost effective and less time consuming. Our in vitro anti-cancer data forms the rationale for us to test heavy terpene complex in breast cancer xenograft model in vivo. Furthermore, fractionation and developing frankincense heavy terpene based breast cancer drug is the major goal of our laboratory.

  17. Frankincense derived heavy terpene cocktail boosting breast cancer cell (MDA-MB-231) deathin vitro

    Institute of Scientific and Technical Information of China (English)

    Faruck Lukmanul Hakkim; Mohammed Al-Buloshi; Jamal Al-Sabahi

    2015-01-01

    Objective:To investigate the anti-cancer effect of frankincense derived heavy oil obtained by Soxhlet extraction method on breast cancer cells (MDA-MB-231), and to study its chemical profile using gas chromatography mass spectrometry analysis. Methods: Hexane was used to extract heavy oil from frankincense resin. Chemical profiling of heavy oil was done using Perkin Elmer Clarus GC system with mass spectrometer.MDA-MB-231 cells were treated with different dilutions (1:1 000, 1:1 500, 1:1 750, 1:2 000, 1:2 250, 1:2 500, 1:2 750, 1:3 000, 1:3 250) of heavy oil for 24 h. The cells were observed by using light microscopy. Cell viability was measured byMTT assay. Results: Gas chromatography mass spectrometry chemical profiling of frankincense derived heavy oil revealed the presence of terpenes such asα-pinene (61.56%),α-amyrin (20.6%),β-amyrin (8.1%),β-phellandrene (1.47%) and camphene (1.04%). Heavy terpene cocktail induced significantMDA-MB-231 cell death at each concentration tested. Noticeably, very low concentration of Soxhlet derived heavy terpenes elicits considerable cytotoxicityon MDA-MB-231cells compared to hydro distillated essential oil derived from frankincense resin. Conclusions: Extracting anti-cancer active principle cocktail by simple Soxhlet method is cost effective and less time consuming. Ourin vitro anti-cancer data forms the rationale for us to test heavy terpene complex in breast cancer xenograft modelin vivo. Furthermore, fractionation and developing frankincense heavy terpene based breast cancer drug is the major goal of our laboratory.

  18. Buspirone, fexofenadine, and omeprazole: Quantification of probe drugs and their metabolites in human plasma

    OpenAIRE

    Gor, Parul; Alnouti, Yazen; Reed, Gregory A.

    2011-01-01

    Probe drugs are critical tools for the measurement of drug metabolism and transport activities in human subjects. Often several probe drugs are administered simultaneously in a —cocktail . This cocktail approach requires efficient analytical methods for the simultaneous quantitation of multiple analytes. We have developed and validated a liquid chromatography-tandem mass spectrometry method for the simultaneous determination of three probe drugs and their metabolites in human plasma. The anal...

  19. Costly third-party punishment in young children.

    Science.gov (United States)

    McAuliffe, Katherine; Jordan, Jillian J; Warneken, Felix

    2015-01-01

    Human adults engage in costly third-party punishment of unfair behavior, but the developmental origins of this behavior are unknown. Here we investigate costly third-party punishment in 5- and 6-year-old children. Participants were asked to accept (enact) or reject (punish) proposed allocations of resources between a pair of absent, anonymous children. In addition, we manipulated whether subjects had to pay a cost to punish proposed allocations. Experiment 1 showed that 6-year-olds (but not 5-year-olds) punished unfair proposals more than fair proposals. However, children punished less when doing so was personally costly. Thus, while sensitive to cost, they were willing to sacrifice resources to intervene against unfairness. Experiment 2 showed that 6-year-olds were less sensitive to unequal allocations when they resulted from selfishness than generosity. These findings show that costly third-party punishment of unfair behavior is present in young children, suggesting that from early in development children show a sophisticated capacity to promote fair behavior.

  20. Institute of party leadership system of the party creation of modern Ukraine

    Directory of Open Access Journals (Sweden)

    I. O. Lavrenyuk

    2015-06-01

    Full Text Available This article based on the study of the theory and practice of party­building in Ukraine examines the role of party leader in the political process and the establishment of parties, implementation of the political power, the development of political consciousness and the formation of modern political elite of our country. It is alleged that party leader – a reputable, recognized in its political environment, the person chosen to lead the party and the ability of citizens to consolidate around a common goal, most effectively represent the interests of a particular social group. It is stated that the defining feature of the phenomenon of party leadership is that under conditions of constant political development and irreversibility of democratic transformations in Ukraine part of leaders of political parties in fact is a powerful talent pool for senior government officials, local government agencies and other administrative institutions. The process of creating games as an integral part of the political process is characterized by a certain way, the mechanism of action of consciousness and culture in the public sphere, which deals with political parties. Regularities component of modern political elite of the party elite acts ­ governing the range of persons in the political party headed by its leader that are directly involved in strategic decision­making, policy formulation of this power, as well as its participation in the political process. We study the classification model of national political parties and the institutions of the party leadership. It is proposed to assess the level of correlation of the party leaders and the general dynamics of the civil society in Ukraine. It is stated that at this time a fundamental change in Ukrainian Party elite has not happened yet. However, there is a long evolution of the institute of the party leadership – a rather difficult process of internal and external, objective and subjective, economic

  1. Looking Under the Hood of Third-Party Punishment Reveals Design for Personal Benefit.

    Science.gov (United States)

    Krasnow, Max M; Delton, Andrew W; Cosmides, Leda; Tooby, John

    2016-03-01

    Third-party intervention, such as when a crowd stops a mugger, is common. Yet it seems irrational because it has real costs but may provide no personal benefits. In a laboratory analogue, the third-party-punishment game, third parties ("punishers") will often spend real money to anonymously punish bad behavior directed at other people. A common explanation is that third-party punishment exists to maintain a cooperative society. We tested a different explanation: Third-party punishment results from a deterrence psychology for defending personal interests. Because humans evolved in small-scale, face-to-face social worlds, the mind infers that mistreatment of a third party predicts later mistreatment of oneself. We showed that when punishers do not have information about how they personally will be treated, they infer that mistreatment of other people predicts mistreatment of themselves, and these inferences predict punishment. But when information about personal mistreatment is available, it drives punishment. This suggests that humans' punitive psychology evolved to defend personal interests.

  2. Third-party punishment increases cooperation in children through (misaligned) expectations and conditional cooperation.

    Science.gov (United States)

    Lergetporer, Philipp; Angerer, Silvia; Glätzle-Rützler, Daniela; Sutter, Matthias

    2014-05-13

    The human ability to establish cooperation, even in large groups of genetically unrelated strangers, depends upon the enforcement of cooperation norms. Third-party punishment is one important factor to explain high levels of cooperation among humans, although it is still somewhat disputed whether other animal species also use this mechanism for promoting cooperation. We study the effectiveness of third-party punishment to increase children's cooperative behavior in a large-scale cooperation game. Based on an experiment with 1,120 children, aged 7 to 11 y, we find that the threat of third-party punishment more than doubles cooperation rates, despite the fact that children are rarely willing to execute costly punishment. We can show that the higher cooperation levels with third-party punishment are driven by two components. First, cooperation is a rational (expected payoff-maximizing) response to incorrect beliefs about the punishment behavior of third parties. Second, cooperation is a conditionally cooperative reaction to correct beliefs that third party punishment will increase a partner's level of cooperation.

  3. Estonian Political Parties in the mid-2010s

    Directory of Open Access Journals (Sweden)

    Lanko Dmitry

    2015-06-01

    Full Text Available The article provides an analysis of political party system of the Republic of Estonia in the mid-2010s. The analysis is based on the works of Moris Duverger. As one might expect, the establishment of proportionate electoral system in Estonia has resulted in the formation of a multi-party system, in which no single party dominates in the parliament even in a short run. The article demonstrates that though Estonian political party system develops in line with the tendencies typical to political party systems of most European countries, some of its elements are more common to postcommunist countries. It indicates that the political party system in Estonia has stabilized throughout the past decade. Today, five sixths of voters support one of the four main political parties. A minority of voters does not consider any of the four dominant parties as a representative of their interests; thus, they vote for parties that had not been previously represented in the parliament. This allowed for two minor political parties to pass into the parliament at 2015 elections: the Estonian Conservative People’s Party, and Free Party. In the long run the minor parties will be able to keep parliamentary seats depending on their ability to build coalitions, either with the three governing parties — Reform Party, Pro Patria and Republic Union, and Social-Democratic Party, or with opposition Centre Party. The article considers the impact of the split in the Estonian society between ethnic Estonians and Russophonic people on political party system. It demonstrates that the majority of Russophonic voters in Estonia support the Centre Party, every major political party in the country has its Russophonic voters, while Estonian United Left Party, which promotes itself as a particular representative of the country’s Russophonic minority, remains a marginal political force.

  4. The Nationalisation of Post-Communist Party Systems

    DEFF Research Database (Denmark)

    Bochsler, Daniel

    2010-01-01

    Party nationalisation, defined as the homogeneity of party strength across a country, has recently become a major issue in research. Even though territory is a salient political question in the post- communist countries in Europe, party nationalisation in these countries has been neglected by the...... provide for a high degree of nationalisation, or where super-presidentialism inhibits the creation of strong nationalised parties....

  5. Evaluation of Mutual Drug-Drug Interaction within Geneva Cocktail for Cytochrome P450 Phenotyping using Innovative Dried Blood Sampling Method.

    Science.gov (United States)

    Bosilkovska, Marija; Samer, Caroline; Déglon, Julien; Thomas, Aurélien; Walder, Bernhard; Desmeules, Jules; Daali, Youssef

    2016-09-01

    Cytochrome P450 (CYP) activity can be assessed using a 'cocktail' phenotyping approach. Recently, we have developed a cocktail (Geneva cocktail) which combines the use of low-dose probes with a low-invasiveness dried blood spots (DBS) sampling technique and a single analytical method for the phenotyping of six major CYP isoforms. We have previously demonstrated that modulation of CYP activity after pre-treatment with CYP inhibitors/inducer could be reliably predicted using Geneva cocktail. To further validate this cocktail, in this study, we have verified whether probe drugs contained in the latter cause mutual drug-drug interactions. In a randomized, four-way, Latin-square crossover study, 30 healthy volunteers received low-dose caffeine, flurbiprofen, omeprazole, dextromethorphan and midazolam (a previously validated combination with no mutual drug-drug interactions); fexofenadine alone; bupropion alone; or all seven drugs simultaneously (Geneva cocktail). Pharmacokinetic profiles of the probe drugs and their metabolites were determined in DBS samples using both conventional micropipette sampling and new microfluidic device allowing for self-sampling. The 90% confidence intervals for the geometric mean ratios of AUC metabolite/AUC probe for CYP probes administered alone or within Geneva cocktail fell within the 0.8-1.25 bioequivalence range indicating the absence of pharmacokinetic interaction. The same result was observed for the chosen phenotyping indices, that is metabolic ratios at 2 hr (CYP1A2, CYP3A) or 3 hr (CYP2B6, CYP2C9, CYP2C19, CYP2D6) post-cocktail administration. DBS sampling could successfully be performed using a new microfluidic device. In conclusion, Geneva cocktail combined with an innovative DBS sampling device can be used routinely as a test for simultaneous CYP phenotyping. © 2016 Nordic Association for the Publication of BCPT (former Nordic Pharmacological Society).

  6. New alliances: why mainstream parties govern with radical right-wing populist parties

    NARCIS (Netherlands)

    S.L. de Lange

    2012-01-01

    This article investigates the recent government participation of a number of radical right-wing populist parties in West European democracies. With the help of coalition formation theories the government coalitions in which these parties have participated are characterised and inferences are made ab

  7. 24 CFR 401.500 - Required notices to third parties and meeting with third parties.

    Science.gov (United States)

    2010-04-01

    ... third parties and meeting with third parties. (a) General. The PAE must solicit, and document the...) and (f) of this section, in form and substance acceptable to HUD, must be provided. The PAE may... Project Number; (ii) The responsible PAE and contact person, including the address and telephone...

  8. The party politics of economic reform: Public opinion, party positions and partisan cleavages

    NARCIS (Netherlands)

    Padgett, Stephen

    2005-01-01

    This article focuses on the capacity of parties to cultivate public opinion to accept welfare state reform. 'Preference shaping', it is argued, depends on the intensity of party 'messages', which will be at their strongest where there are sharply defined partisan cleavages in opinion. The aversion o

  9. Nationalism, territory and extremism in party politics

    DEFF Research Database (Denmark)

    Bourne, Angela

    In the paper I address the empirical puzzle arising from different responses by political authorities in Spain and the UK to the existence of political parties integrated in the terrorist groups Euskadi Ta Askatasuna (ETA, Basque Homeland and Freedom) and the Irish Republican Army. More specifica......In the paper I address the empirical puzzle arising from different responses by political authorities in Spain and the UK to the existence of political parties integrated in the terrorist groups Euskadi Ta Askatasuna (ETA, Basque Homeland and Freedom) and the Irish Republican Army. More...... for realization of free speech rights and representation is also emphasized, and where proscription is seen as inimical to resolution of conflict underpinning violence. In the context of party competition, a winning coalition is required for one discourse to predominate. However, I also argued that both ideas...

  10. Secure Two-Party Computational Geometry

    Institute of Scientific and Technical Information of China (English)

    Shun-Dong Li; Yi-Qi Dai

    2005-01-01

    Secure Multi-party Computation has been a research focus in international cryptographic community in recent years. In this paper the authors investigate how some computational geometric problems could be solved in a cooperative environment, where two parties need to solve a geometric problem based on their joint data, but neither wants to disclose its private data to the other party. These problems are the distance between two private points, the relation between a private point and a circle area, the relation between a private point and an ellipse area and the shortest distance between two point sets. The paper gives solutions to these specific geometric problems, and in doing so a building block is developed,the protocol for the distance between two private points, that is also useful in the solutions to other geometric problems and combinatorial problems.

  11. Party Change in Chile in Comparative Perspective

    Directory of Open Access Journals (Sweden)

    Alan Angell

    2003-01-01

    Full Text Available Abstract This article looks at the changes in party systems generally in modern democracies, and argues that many trends observed internationally - growing mistrust of parties, growing electoral de-alignment - are also observed in Chile. Hence any analysis of party change in Chile has to take into account what is happening in other countries with well-established party systems. The article argues that the comparison with the past tends to be limited to the exceptional 1964-1973 period and that a more extended analysis points to many continuities in the Chilean party system. Competing arguments over whether there is new party cleavage in Chile based on the opposition between support for authoritarianism or support for democracy are also examined*.Resumen Este artículo estudia los cambios generales en los sistemas de partidos en las democracias modernas y argumenta que muchas de las tendencias que se observan internacionalmente -como el aumento en la desconfianza en los partidos, o desalineaciones electorales cada vez mayores- también se observan en Chile. De esta forma, cualquier análisis de cambios en el sistema de partidos de Chile tiene que considerar lo que está sucediendo en otros países con sistemas de partidos bien establecidos. El artículo postula que la comparación del sistema de partidos chileno con el pasado reciente tiende a estar limitado al período excepcional 1964-1973 y que un análisis más extendido implicaría muchas más continuidades de las esperadas. También se cuestiona las nuevas discusiones sobre si existe o no una nueva fisura partidaria, basada en la oposición entre el apoyo y rechazo al sistema autoritario, o el apoyo a la democracia.

  12. 'To quarterback behind the scenes, third-party efforts': the tobacco industry and the Tea Party.

    Science.gov (United States)

    Fallin, Amanda; Grana, Rachel; Glantz, Stanton A

    2014-07-01

    The Tea Party, which gained prominence in the USA in 2009, advocates limited government and low taxes. Tea Party organisations, particularly Americans for Prosperity and FreedomWorks, oppose smoke-free laws and tobacco taxes. We used the Legacy Tobacco Documents Library, the Wayback Machine, Google, LexisNexis, the Center for Media and Democracy and the Center for Responsive Politics (opensecrets.org) to examine the tobacco companies' connections to the Tea Party. Starting in the 1980s, tobacco companies worked to create the appearance of broad opposition to tobacco control policies by attempting to create a grassroots smokers' rights movement. Simultaneously, they funded and worked through third-party groups, such as Citizens for a Sound Economy, the predecessor of AFP and FreedomWorks, to accomplish their economic and political agenda. There has been continuity of some key players, strategies and messages from these groups to Tea Party organisations. As of 2012, the Tea Party was beginning to spread internationally. Rather than being a purely grassroots movement that spontaneously developed in 2009, the Tea Party has developed over time, in part through decades of work by the tobacco industry and other corporate interests. It is important for tobacco control advocates in the USA and internationally, to anticipate and counter Tea Party opposition to tobacco control policies and ensure that policymakers, the media and the public understand the longstanding connection between the tobacco industry, the Tea Party and its associated organisations. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

  13. Analysis of the benefits of vitamin cocktails in treating Charcot-Marie-Tooth disease type 1A.

    Science.gov (United States)

    Kaya, Ferdinand; Belin, Sophie; Micallef, Joelle; Blin, Olivier; Fontés, Michel

    2008-08-01

    We recently proposed that the use of high doses of ascorbic acid (AA) could constitute the first potential treatment for Charcot-Marie-Tooth disease type 1A (CMT1A).4 We investigated the potential benefits of using cocktails of vitamins for CMT1A therapy. We used transient transfection of Schwann cells with a construction placing the expression of a reporter gene under the control of the Schwann cell-specific promoter of PMP22. Transfected cells were cultured with or without addition of ascorbic acid, vitamin A, vitamin E, or a cocktail of these vitamins. Adding vitamin A or E counteracts the effect of ascorbic acid in inhibiting PMP22 expression. We thus recommend that vitamins A and E should not be included in combination with AA in clinical trials.

  14. Role of quenching on alpha/beta separation in liquid scintillation counting for several high capacity cocktails

    Energy Technology Data Exchange (ETDEWEB)

    Pujol, L.; Sanchez-Cabeza, J.-A. [Universidad Autonoma de Barcelona (Spain). Facultad de Ciencias

    1997-04-01

    The optimization of alpha/beta separation in liquid scintillation using pulse shape analysis is convenient for the simultaneous determination of alpha and beta emitters in natural water and other samples. In this work, alpha/beta separation was studied for different scintillant/vial combinations and it was observed that both the optimum pulse shape discrimination level and the total interference value (that is, the summed relative interference between alpha and beta spectra) were dependent on the sample quenching and independent of the scintillant/vial combination. These results provide a simple method for modifying the counting configuration, such as a change in the cocktail, vial or sample characteristics, without the need to perform exhaustive parameter optimizations. Also, it was observed that, for our counting conditions, the combination of Ultima Gold AB scintillation cocktail with Zinsser low diffusion vials presented the lowest total interference, namely 0.94 {+-} 0.28%, which is insignificant for the counting of environmental samples. (Author).

  15. A NOVEL STRAIN OF Aspergillus niger PRODUCING A COCKTAIL OF HYDROLYTIC DEPOLYMERISING ENZYMES FOR THE PRODUCTION OF SECOND GENERATION BIOFUELS

    Directory of Open Access Journals (Sweden)

    Namita Bansal

    2011-02-01

    Full Text Available The screening and isolation of fungi producing a cocktail of hydrolytic enzymes was studied. Among the various isolates obtained from different soil samples, a strain NS-2 was selected. The phylogenetic analysis of this strain showed highest homology (99% with Aspergillus niger. It was capable of producing cellulolytic, hemicellulolytic, amylolytic, and pectinolytic enzymes in appreciable titers on wheat bran based liquid and solid state media. The mixture of enzymes produced by this organism could effectively hydrolyze various domestic waste residues, revealing conversion efficiencies of 89 to 92% and produced high reducing sugar yields of 0.48 to 0.66 g/g of dry residue. This enzyme cocktail could potentially find a significant application in the conversion of agricultural and other waste residues having cellulose, hemicellulose, starch, and pectin as carbohydrates to produce simpler sugars which can be fermented for the production of second generation biofuels.

  16. A Review of 1982 Constitution Regulations on Political Parties

    Directory of Open Access Journals (Sweden)

    Rıfat KARAKOÇ

    2013-12-01

    Full Text Available Political parties are the vital elements of modern democratic regimes. Today, for a democracy, the existence of more than one political parties and fair and free competition of these parties is a necessity. In Turkey, until 1946, singe party regime has been employed and after this year, Turkey shifted to multi-parties regime, which is required for real democracy. Since 1946, political parties were counted and regulated in 1961 and 1982 constitutions. In this study; the relation between political parties and democracy, the regulations of 1982 constitution on political parties, the amendments in these regulation in time and lastly, the judgments of constitution regarding the closure cases of political parties will be examined.

  17. THE VALIDITY OF MYCOBACTERIUM TUBERCULOSIS ANTIGENS COCKTAIL: ESAT-6, CFP-10 AND MPT64 IN SPUTUM AND CEREBROSPINAL FLUID FOR PULMONARY TUBERCULOSIS AND TUBERCULOUS MENINGITIS DIAGNOSIS

    OpenAIRE

    Dewi Kartika Turbawaty; Nenny Gustiani; Livia Noviani; Ida Parwati

    2015-01-01

    Objective: To determine the validity of tuberculosis (TB) antigen cocktail (ESAT-6, CFP-10 and MPT64) for pulmonary tuberculosis and TB meningitis diagnosis. Methods: This is a descriptive observational study design. The study was conducted at the Clinical Pathology Laboratory of Dr. Hasan Sadikin General Hospital during September 2012 until March 2013 for the pulmonary tuberculosis study and from January 2014 to May 2014 for the TB meningitis study. The TB antigen cocktail rapid imm...

  18. The Phonetic Rhetoric in The Garden Party

    Institute of Scientific and Technical Information of China (English)

    汪婷; 尹静

    2015-01-01

    Katherine Mansfield is a prominent modernist writer. The Garden Party is one of important short stories she wrote in 1921. The story is full of musicality. What’s more, the phonation, stress and intonation of words make the passage possess consis⁃tency with the growth of Laura’s inner heart, that is from excitement, hesitation to peaceful after realization.

  19. The Other Boston Tea Party. Teacher's Guide.

    Science.gov (United States)

    Rosal, Lorenca Consuelo; And Others

    A teachers' guide is presented that accompanies the video production of "The Other Boston Tea Party," a play used to teach about the U.S. Constitution and American legal and political systems. The play combines a comedy of manners with an historical approach to the issues surrounding the Federalist/Anti-Federalist debate over…

  20. 34 CFR 682.203 - Responsible parties.

    Science.gov (United States)

    2010-07-01

    ... 34 Education 3 2010-07-01 2010-07-01 false Responsible parties. 682.203 Section 682.203 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION FEDERAL FAMILY EDUCATION LOAN (FFEL) PROGRAM General Provisions § 682.203...

  1. Social media, parties, and political inequalities

    NARCIS (Netherlands)

    Jacobs, K.T.E.; Spierings, C.H.B.M.

    2016-01-01

    This book examines how social media have transformed politics in established democracies. Specifically, the authors examine the influence of the unique qualities of social media on the power balance between and within parties. They present a general theory as well as an in-depth case study of the Ne

  2. Third-Party Contract Negotiations for CEOs?

    Science.gov (United States)

    Howell, Joe

    1990-01-01

    New and incumbent presidents sometimes are reticent about discussing the specifics of compensation and benefit packages. Third-party negotiators might help bring these issues into the open. A negotiation process and nine specific topics for inclusion in negotiation discussions are outlined. (Author/MSE)

  3. Political party affiliation, political ideology and mortality.

    Science.gov (United States)

    Pabayo, Roman; Kawachi, Ichiro; Muennig, Peter

    2015-05-01

    Ecological and cross-sectional studies have indicated that conservative political ideology is associated with better health. Longitudinal analyses of mortality are needed because subjective assessments of ideology may confound subjective assessments of health, particularly in cross-sectional analyses. Data were derived from the 2008 General Social Survey-National Death Index data set. Cox proportional analysis models were used to determine whether political party affiliation or political ideology was associated with time to death. Also, we attempted to identify whether self-reported happiness and self-rated health acted as mediators between political beliefs and time to death. In this analysis of 32,830 participants and a total follow-up time of 498,845 person-years, we find that political party affiliation and political ideology are associated with mortality. However, with the exception of independents (adjusted HR (AHR)=0.93, 95% CI 0.90 to 0.97), political party differences are explained by the participants' underlying sociodemographic characteristics. With respect to ideology, conservatives (AHR=1.06, 95% CI 1.01 to 1.12) and moderates (AHR=1.06, 95% CI 1.01 to 1.11) are at greater risk for mortality during follow-up than liberals. Political party affiliation and political ideology appear to be different predictors of mortality. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

  4. LEGO for Two-Party Secure Computation

    DEFF Research Database (Denmark)

    Nielsen, Jesper Buus; Orlandi, Claudio

    2009-01-01

    This paper continues the recent line of work of making Yao’s garbled circuit approach to two-party computation secure against an active adversary. We propose a new cut-and-choose based approach called LEGO (Large Efficient Garbled-circuit Optimization): It is specifically aimed at large circuits...

  5. 12 CFR 1705.3 - Eligible parties.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 7 2010-01-01 2010-01-01 false Eligible parties. 1705.3 Section 1705.3 Banks and Banking OFFICE OF FEDERAL HOUSING ENTERPRISE OVERSIGHT, DEPARTMENT OF HOUSING AND URBAN... defined in section 15(a) of the Agricultural Marketing Act, 12 U.S.C. 1141j(a), with not more than...

  6. Electoral system, pesonal votes, and party choice

    DEFF Research Database (Denmark)

    Thomsen, Søren Risbjerg

    Using local elections in Denmark as an example this paper shows that individual party choice is influenced both by individual level, municipality level, and national level characteristics. Some hypotheses about the effects of the electoral system on personal votes derived from a theory by Carey...

  7. Capuchin Monkeys Judge Third-Party Reciprocity

    Science.gov (United States)

    Anderson, James R.; Takimoto, Ayaka; Kuroshima, Hika; Fujita, Kazuo

    2013-01-01

    Increasing interest is being shown in how children develop an understanding of reciprocity in social exchanges and fairness in resource distribution, including social exchanges between third parties. Although there are descriptions of reciprocity on a one-to-one basis in other species, whether nonhumans detect reciprocity and violations of…

  8. Cornerstone Third Party Alternative Cost Study

    Energy Technology Data Exchange (ETDEWEB)

    Vrieling, P. Douglas [Sandia National Lab. (SNL-CA), Livermore, CA (United States)

    2013-10-15

    The attached cost analyses sheets have been developed for use in planning during the Third Party Alternative study currently underway for the Sandia CREATE project. This cost analysis builds upon the previously submitted base estimate dated June 14, 2013 and includes comparison information collected during a Market Validation exercise conducted in August/September 2013.

  9. Electoral system, pesonal votes, and party choice

    DEFF Research Database (Denmark)

    Thomsen, Søren Risbjerg

    Using local elections in Denmark as an example this paper shows that individual party choice is influenced both by individual level, municipality level, and national level characteristics. Some hypotheses about the effects of the electoral system on personal votes derived from a theory by Carey...

  10. Analysis of a Multi-component Multi-stage Malaria Vaccine Candidate--Tackling the Cocktail Challenge.

    Directory of Open Access Journals (Sweden)

    Alexander Boes

    Full Text Available Combining key antigens from the different stages of the P. falciparum life cycle in the context of a multi-stage-specific cocktail offers a promising approach towards the development of a malaria vaccine ideally capable of preventing initial infection, the clinical manifestation as well as the transmission of the disease. To investigate the potential of such an approach we combined proteins and domains (11 in total from the pre-erythrocytic, blood and sexual stages of P. falciparum into a cocktail of four different components recombinantly produced in plants. After immunization of rabbits we determined the domain-specific antibody titers as well as component-specific antibody concentrations and correlated them with stage specific in vitro efficacy. Using purified rabbit immune IgG we observed strong inhibition in functional in vitro assays addressing the pre-erythrocytic (up to 80%, blood (up to 90% and sexual parasite stages (100%. Based on the component-specific antibody concentrations we calculated the IC50 values for the pre-erythrocytic stage (17-25 μg/ml, the blood stage (40-60 μg/ml and the sexual stage (1.75 μg/ml. While the results underline the feasibility of a multi-stage vaccine cocktail, the analysis of component-specific efficacy indicates significant differences in IC50 requirements for stage-specific antibody concentrations providing valuable insights into this complex scenario and will thereby improve future approaches towards malaria vaccine cocktail development regarding the selection of suitable antigens and the ratios of components, to fine tune overall and stage-specific efficacy.

  11. Analysis of a Multi-component Multi-stage Malaria Vaccine Candidate—Tackling the Cocktail Challenge

    Science.gov (United States)

    Voepel, Nadja; Edgue, Gueven; Beiss, Veronique; Kapelski, Stephanie; Fendel, Rolf; Scheuermayer, Matthias; Pradel, Gabriele; Bolscher, Judith M.; Behet, Marije C.; Dechering, Koen J.; Hermsen, Cornelus C.; Sauerwein, Robert W.; Schillberg, Stefan; Reimann, Andreas; Fischer, Rainer

    2015-01-01

    Combining key antigens from the different stages of the P. falciparum life cycle in the context of a multi-stage-specific cocktail offers a promising approach towards the development of a malaria vaccine ideally capable of preventing initial infection, the clinical manifestation as well as the transmission of the disease. To investigate the potential of such an approach we combined proteins and domains (11 in total) from the pre-erythrocytic, blood and sexual stages of P. falciparum into a cocktail of four different components recombinantly produced in plants. After immunization of rabbits we determined the domain-specific antibody titers as well as component-specific antibody concentrations and correlated them with stage specific in vitro efficacy. Using purified rabbit immune IgG we observed strong inhibition in functional in vitro assays addressing the pre-erythrocytic (up to 80%), blood (up to 90%) and sexual parasite stages (100%). Based on the component-specific antibody concentrations we calculated the IC50 values for the pre-erythrocytic stage (17–25 μg/ml), the blood stage (40–60 μg/ml) and the sexual stage (1.75 μg/ml). While the results underline the feasibility of a multi-stage vaccine cocktail, the analysis of component-specific efficacy indicates significant differences in IC50 requirements for stage-specific antibody concentrations providing valuable insights into this complex scenario and will thereby improve future approaches towards malaria vaccine cocktail development regarding the selection of suitable antigens and the ratios of components, to fine tune overall and stage-specific efficacy. PMID:26147206

  12. Analysis of a Multi-component Multi-stage Malaria Vaccine Candidate--Tackling the Cocktail Challenge.

    Science.gov (United States)

    Boes, Alexander; Spiegel, Holger; Voepel, Nadja; Edgue, Gueven; Beiss, Veronique; Kapelski, Stephanie; Fendel, Rolf; Scheuermayer, Matthias; Pradel, Gabriele; Bolscher, Judith M; Behet, Marije C; Dechering, Koen J; Hermsen, Cornelus C; Sauerwein, Robert W; Schillberg, Stefan; Reimann, Andreas; Fischer, Rainer

    2015-01-01

    Combining key antigens from the different stages of the P. falciparum life cycle in the context of a multi-stage-specific cocktail offers a promising approach towards the development of a malaria vaccine ideally capable of preventing initial infection, the clinical manifestation as well as the transmission of the disease. To investigate the potential of such an approach we combined proteins and domains (11 in total) from the pre-erythrocytic, blood and sexual stages of P. falciparum into a cocktail of four different components recombinantly produced in plants. After immunization of rabbits we determined the domain-specific antibody titers as well as component-specific antibody concentrations and correlated them with stage specific in vitro efficacy. Using purified rabbit immune IgG we observed strong inhibition in functional in vitro assays addressing the pre-erythrocytic (up to 80%), blood (up to 90%) and sexual parasite stages (100%). Based on the component-specific antibody concentrations we calculated the IC50 values for the pre-erythrocytic stage (17-25 μg/ml), the blood stage (40-60 μg/ml) and the sexual stage (1.75 μg/ml). While the results underline the feasibility of a multi-stage vaccine cocktail, the analysis of component-specific efficacy indicates significant differences in IC50 requirements for stage-specific antibody concentrations providing valuable insights into this complex scenario and will thereby improve future approaches towards malaria vaccine cocktail development regarding the selection of suitable antigens and the ratios of components, to fine tune overall and stage-specific efficacy.

  13. Frankincense derived heavy terpene cocktail boosting breast cancer cell (MDA-MB-231 death in vitro

    Directory of Open Access Journals (Sweden)

    Faruck Lukmanul Hakkim

    2015-10-01

    Conclusions: Extracting anti-cancer active principle cocktail by simple Soxhlet method is cost effective and less time consuming. Our in vitro anti-cancer data forms the rationale for us to test heavy terpene complex in breast cancer xenograft model in vivo. Furthermore, fractionation and developing frankincense heavy terpene based breast cancer drug is the major goal of our laboratory.

  14. Thermal conductivity of the cryoprotective cocktail DP6 in cryogenic temperatures, in the presence and absence of synthetic ice modulators.

    Science.gov (United States)

    Ehrlich, Lili E; Malen, Jonathan A; Rabin, Yoed

    2016-10-01

    The thermal conductivity of the cryoprotective agent (CPA) cocktail DP6 in combination with synthetic ice modulators (SIMs) is measured in this study, using a transient hot-wire method. DP6 is a mixture of 3 M dimethyl sulfoxide (DMSO) and 3 M propylene glycol, which received significant attention in the cryobiology community in recent years. Tested SIMs include 6% 1,3Cyclohexanediol, 6% 2,3Butanediol, and 12% PEG400 (percentage by volume). This study integrates the scanning cryomacroscope for visual verification of crystallization and vitrification events. It is demonstrated that the thermal conductivity of the vitrifying CPA cocktail decreases monotonically with the decreasing temperature down to -180 °C. By contrast, the thermal conductivity of the crystalline material increases with decreasing temperature in the same temperature range. Results of this study demonstrate that the thermal conductivity may vary by three fold between the amorphous and crystalline phases of DP6 below the glass transition temperature of DP6 (Tg = -119 °C). The selected SIMs demonstrate the ability to inhibit crystallization in DP6, even at subcritical cooling rates. An additional ice suppression capability is observed by the Euro-Collins as a vehicle solution, disproportionate to its volume ratio in the cocktail. The implication of the observed thermal conductivity differences between the amorphous and crystalline phases of the same cocktail on cryopreservation simulations is significant in some cases and must be taken into account in thermal analyses of cryopreservation protocols.

  15. Collective members in West European political parties

    Directory of Open Access Journals (Sweden)

    Gutiérrez, José Manuel

    2016-06-01

    Full Text Available This article focuses on the collective members of political parties. Collective members can be defined as organizations having both full self-government rights and entitlements to decision-making processes in the political parties in which they are embedded. This article proposes a basic descriptive typology of collective members that takes into consideration their sociopolitical nature (party political or functional and their pattern of articulation within the party in which they are embedded (whether they help to articulate the whole party structure or not. It also illustrates these subtypes by examining European organizations that meet (or are close to meeting the main defining characteristics of collective members. This examination is based on the analysis of all parties that attained at least 4% of the votes in the last national parliamentary elections in 10 European countries. The article concludes with an analysis of the determinants and prospects of this type of party structure.Este artículo estudia los miembros colectivos de los partidos políticos. Los miembros colectivos pueden ser definidos como organizaciones que poseen a la vez derechos de autogobierno plenos y derecho a participar en los procesos de toma decisiones en el partido político en el que están integrados. El artículo propone una tipología descriptiva de los miembros colectivos que tiene en cuenta su naturaleza sociopolítica (partidista o funcional y su patrón de articulación dentro del partido en el que están integrados (si ayudan o no a articular el conjunto de la estructura partidaria. Asimismo, el artículo ilustra estos subtipos a partir del examen de varias organizaciones políticas europeas que cumplen (o se acercan a cumplir las características definitorias de los miembros colectivos. Este análisis se basa en el examen de todas las organizaciones políticas que alcanzaron más de un 4% de los votos en las últimas elecciones parlamentarias de 10 pa

  16. A Society of Patriotic Ladies: the Edenton Tea Party

    OpenAIRE

    Cusack, Tricia

    2016-01-01

    This paper examines a relatively little-known revolutionary event in British colonial America known as the Edenton tea party that took place on 25 October in Edenton, North Carolina ten months after its more famous namesake, the Boston Tea Party. Like the Boston tea party the Edenton tea party was an explicitly political event aimed at a new tax on tea imposed by the East India Company. However, unlike the Boston tea party the Edenton tea party solely involved women, the key participants bein...

  17. Populism in Iceland: Has the Progressive Party turned populist?

    Directory of Open Access Journals (Sweden)

    Eiríkur Bergmann

    2015-06-01

    Full Text Available Though nationalism has always been strong in Iceland, populist political parties did not emerge as a viable force until after the financial crisis of 2008. On wave of the crisis a completely renewed leadership took over the country’s old agrarian party, the Progressive Party (PP, which was rapidly transformed in a more populist direction. Still the PP is perhaps more firmly nationalist than populist. However, when analyzing communicational changes of the new postcrisis leadership it is unavoidable to categorize the party amongst at least the softer version of European populist parties, perhaps closest to the Norwegian Progress Party.

  18. Impact of a Microbial Cocktail Used as a Starter Culture on Cocoa Fermentation and Chocolate Flavor

    Directory of Open Access Journals (Sweden)

    Igor Magalhães da Veiga Moreira

    2017-05-01

    Full Text Available Chocolate production suffered a vast impact with the emergence of the “witches’ broom” disease in cocoa plants. To recover cocoa production, many disease-resistant hybrid plants have been developed. However, some different cocoa hybrids produce cocoa beans that generate chocolate with variable quality. Fermentation of cocoa beans is a microbiological process that can be applied for the production of chocolate flavor precursors, leading to overcoming the problem of variable chocolate quality. The aim of this work was to use a cocktail of microorganisms as a starter culture on the fermentation of the ripe cocoa pods from PH15 cocoa hybrid, and evaluate its influence on the microbial communities present on the fermentative process on the compounds involved during the fermentation, and to perform the chocolate sensorial characterization. According to the results obtained, different volatile compounds were identified in fermented beans and in the chocolate produced. Bitterness was the dominant taste found in non-inoculated chocolate, while chocolate made with inoculated beans showed bitter, sweet, and cocoa tastes. 2,3-Butanediol and 2,3-dimethylpyrazine were considered as volatile compounds making the difference on the flavor of both chocolates. Saccharomyces cerevisiae UFLA CCMA 0200, Lactobacillus plantarum CCMA 0238, and Acetobacter pasteurianus CCMA 0241 are proposed as starter cultures for cocoa fermentation.

  19. Impact of a Microbial Cocktail Used as a Starter Culture on Cocoa Fermentation and Chocolate Flavor.

    Science.gov (United States)

    Magalhães da Veiga Moreira, Igor; de Figueiredo Vilela, Leonardo; da Cruz Pedroso Miguel, Maria Gabriela; Santos, Cledir; Lima, Nelson; Freitas Schwan, Rosane

    2017-05-09

    Chocolate production suffered a vast impact with the emergence of the "witches' broom" disease in cocoa plants. To recover cocoa production, many disease-resistant hybrid plants have been developed. However, some different cocoa hybrids produce cocoa beans that generate chocolate with variable quality. Fermentation of cocoa beans is a microbiological process that can be applied for the production of chocolate flavor precursors, leading to overcoming the problem of variable chocolate quality. The aim of this work was to use a cocktail of microorganisms as a starter culture on the fermentation of the ripe cocoa pods from PH15 cocoa hybrid, and evaluate its influence on the microbial communities present on the fermentative process on the compounds involved during the fermentation, and to perform the chocolate sensorial characterization. According to the results obtained, different volatile compounds were identified in fermented beans and in the chocolate produced. Bitterness was the dominant taste found in non-inoculated chocolate, while chocolate made with inoculated beans showed bitter, sweet, and cocoa tastes. 2,3-Butanediol and 2,3-dimethylpyrazine were considered as volatile compounds making the difference on the flavor of both chocolates. Saccharomyces cerevisiae UFLA CCMA 0200, Lactobacillus plantarum CCMA 0238, and Acetobacter pasteurianus CCMA 0241 are proposed as starter cultures for cocoa fermentation.

  20. Mechanistic modeling of enzymatic hydrolysis of cellulose integrating substrate morphology and cocktail composition.

    Science.gov (United States)

    Huron, Maïté; Hudebine, Damien; Lopes Ferreira, Nicolas; Lachenal, Dominique

    2016-05-01

    A mechanistic model of enzymatic hydrolysis taking into account the morphology of the cellulosic particles and its evolution with time was developed. The individual behavior of the main enzymes involved in the reaction (cellobiohydrolases, endoglucanases, and β-glucosidases), as well as synergy effects, were also included. A large panel of experimental tests was done to fit and validate the model. This database included different enzymes mixtures and operating conditions and allowed to determine and compare with accuracy the adsorption and kinetic parameters of the different enzymes. Model predictions on short hydrolysis times were very satisfactory. On longer times, a deactivation constant was added to represent the hydrolysis slowdown. The model also allowed to predict the impact of enzymes ratios and initial substrate parameters (chain length distribution, polymerization degree) on hydrolysis, and to follow the evolution of these parameters with time. This model revealed general trends on the impact of cellulose morphology on hydrolysis. It is a useful tool to better understand the mechanisms involved in enzymatic hydrolysis of cellulose and to determine optimal cellulolytic cocktails for process design. © 2015 Wiley Periodicals, Inc.

  1. ACID EVAPORATION OF ULTIMA GOLD TM AB LIQUID SCINTILLATION COCKTAIL RESIDUE

    Energy Technology Data Exchange (ETDEWEB)

    Kyser, E.; Fondeur, F.; Crump, S.

    2011-12-21

    Prior analyses of samples from the F/H Lab solutions showed the presence of diisopropylnapthalene (DIN), a major component of Ultima Gold{trademark} AB liquid scintillation cocktail (LSC). These solutions are processed through H-Canyon Tank 10.5 and ultimately through the 17.8E evaporator. Similar solutions originated in SRNL streams sent to the same H Canyon tanks. This study examined whether the presence of these organics poses a process-significant hazard for the evaporator. Evaporation and calorimetry testing of surrogate samples containing 2000 ppm of Ultima Gold{trademark} AB LSC in 8 M nitric acid have been completed. These experiments showed that although reactions between nitric acid and the organic components do occur, they do not appear to pose a significant hazard for runaway reactions or generation of energetic compounds in canyon evaporators. The amount of off-gas generated was relatively modest and appeared to be well within the venting capacity of the H-Canyon evaporators. A significant fraction of the organic components likely survives the evaporation process primarily as non-volatile components that are not expected to represent any new process concerns during downstream operations such as neutralization. Laboratory Waste solutions containing minor amounts of DIN can be safely received, stored, transferred, and processed through the canyon waste evaporator.

  2. A Comparison of Blood Factor XII Autoactivation in Buffer, Protein Cocktail, Serum, and Plasma Solutions

    Science.gov (United States)

    Golas, Avantika; Yeh, Chyi-Huey Josh; Pitakjakpipop, Harit; Siedlecki, Christopher A.; Vogler, Erwin A.

    2012-01-01

    Activation of blood plasma coagulation in vitro by contact with material surfaces is demonstrably dependent on plasma-volume-to-activator-surface-area ratio. The only plausible explanation consistent with current understanding of coagulation-cascade biochemistry is that procoagulant stimulus arising from the activation complex of the intrinsic pathway is dependent on activator surface area. And yet, it is herein shown that activation of the blood zymogen factor XII (Hageman factor, FXII) dissolved in buffer, protein cocktail, heat-denatured serum, and FXI deficient plasma does not exhibit activator surface-area dependence. Instead, a highly-variable burst of procoagulant-enzyme yield is measured that exhibits no measurable kinetics, sensitivity to mixing, or solution-temperature dependence. Thus, FXII activation in both buffer and protein-containing solutions does not exhibit characteristics of a biochemical reaction but rather appears to be a “mechanochemical” reaction induced by FXII molecule interactions with hydrophilic activator particles that do not formally adsorb blood proteins from solution. Results of this study strongly suggest that activator surface-area dependence observed in contact activation of plasma coagulation does not solely arise at the FXII activation step of the intrinsic pathway. PMID:23117212

  3. Oral T4-like phage cocktail application to healthy adult volunteers from Bangladesh

    Energy Technology Data Exchange (ETDEWEB)

    Sarker, Shafiqul Alam, E-mail: sasarker@icddrb.org [International Centre for Diarrhoeal Diseases Research, Bangladesh (icddr,b), 68 Shaheed Tajuddin Ahmed Sharani, Mohakhali, Dhaka 1212 (Bangladesh); McCallin, Shawna; Barretto, Caroline [Nestle Research Centre, Nestec Ltd., Vers-chez-les-Blanc, CH-1000 Lausanne 26 (Switzerland); Berger, Bernard, E-mail: bernard.berger@rdls.nestle.com [Nestle Research Centre, Nestec Ltd., Vers-chez-les-Blanc, CH-1000 Lausanne 26 (Switzerland); Pittet, Anne-Cecile [Nestle Research Centre, Nestec Ltd., Vers-chez-les-Blanc, CH-1000 Lausanne 26 (Switzerland); Sultana, Shamima, E-mail: shamima@icddrb.org [International Centre for Diarrhoeal Diseases Research, Bangladesh (icddr,b), 68 Shaheed Tajuddin Ahmed Sharani, Mohakhali, Dhaka 1212 (Bangladesh); Krause, Lutz, E-mail: ltz.krause@gmail.com [Nestle Research Centre, Nestec Ltd., Vers-chez-les-Blanc, CH-1000 Lausanne 26 (Switzerland); Huq, Sayeda, E-mail: sayeeda@mail.icddrb.org [International Centre for Diarrhoeal Diseases Research, Bangladesh (icddr,b), 68 Shaheed Tajuddin Ahmed Sharani, Mohakhali, Dhaka 1212 (Bangladesh); Bibiloni, Rodrigo, E-mail: Rodrigo.Bibiloni@agresearch.co.nz [Nestle Research Centre, Nestec Ltd., Vers-chez-les-Blanc, CH-1000 Lausanne 26 (Switzerland); Bruttin, Anne, E-mail: anne.bruttin@rdls.nestle.com [Nestle Research Centre, Nestec Ltd., Vers-chez-les-Blanc, CH-1000 Lausanne 26 (Switzerland); Reuteler, Gloria, E-mail: gloria.reuteler@rdls.nestle.com [Nestle Research Centre, Nestec Ltd., Vers-chez-les-Blanc, CH-1000 Lausanne 26 (Switzerland); Bruessow, Harald, E-mail: harald.bruessow@rdls.nestle.com [Nestle Research Centre, Nestec Ltd., Vers-chez-les-Blanc, CH-1000 Lausanne 26 (Switzerland)

    2012-12-20

    The genomic diversity of 99 T4-like coliphages was investigated by sequencing an equimolar mixture with Illumina technology and screening them against different databases for horizontal gene transfer and undesired genes. A 9-phage cocktail was given to 15 healthy adults from Bangladesh at a dose of 3 Multiplication-Sign 10{sup 9} and 3 Multiplication-Sign 10{sup 7} plaque-forming units and placebo respectively. Phages were detected in 64% of the stool samples when subjects were treated with higher titer phage, compared to 30% and 28% with lower-titer phage and placebo, respectively. No Escherichia coli was present in initial stool samples, and no amplification of phage was observed. One percent of the administered oral phage was recovered from the feces. No adverse events were observed by self-report, clinical examination, or from laboratory tests for liver, kidney, and hematology function. No impact of oral phage was seen on the fecal microbiota composition with respect to bacterial 16S rRNA from stool.

  4. In vitro interaction cocktail assay for nine major cytochrome P450 enzymes with 13 probe reactions and a single LC/MSMS run: analytical validation and testing with monoclonal anti-CYP antibodies.

    Science.gov (United States)

    Tolonen, Ari; Petsalo, Aleksanteri; Turpeinen, Miia; Uusitalo, Jouko; Pelkonen, Olavi

    2007-07-01

    A sensitive and rugged LC/MSMS method was developed for a comprehensive in vitro metabolic interaction screening assay with N-in-1 approach reported earlier. A cocktail consisting of ten cytochrome P450 (CYP)-selective probe substrates with known kinetic, metabolic and interaction properties in vivo was incubated in a pool of human liver microsomes, and metabolites of melatonin (CYP1A2), coumarin (CYP2A6), bupropion (CYP2B6), amodiaquine (CYP2C8) tolbutamide (CYP2C9), omeprazole (CYP2C19 and CYP3A4), dextromethorphan (CYP2D6), chlorzoxazone (CYP2E1), midazolam (CYP3A4) and testosterone (CYP3A4) were simultaneously analysed with a single LC/MSMS run. Altogether, 13 metabolites and internal standard phenacetin were analysed in multiple reaction mode. Polarity switching mode was utilized to acquire negative ion mode electrospray data for hydroxychlorzoxazone and positive ionization data for the rest of the analytes. Fast gradient elution was applied, giving total injection cycle of 8 min. The method was modified for two different LC/MSMS systems, and was validated for linear range, detection limit, accuracy and precision for each metabolite. In addition, cocktail inhibition system was further tested using monoclonal anti-CYP antibodies as inhibitors for each probe reaction. Copyright (c) 2007 John Wiley & Sons, Ltd.

  5. Political Market Orientation and Strategic Party Postures in Danish Political Parties

    DEFF Research Database (Denmark)

    Ormrod, Robert P.; Henneberg, Stephan

    2011-01-01

    Ideologist. Thus, Party A exhibits a mismatch between strategic orientation and implemented organisational profile, based on configuration theory. The results were generally stable across political activity levels. Originality/value – The study contributes to understanding the concept of market orientation...... variables needs to be established to assess the extent to which the organisational alignment results in competitive advantages for a party. Practical implications – Whilst there exists a general cohesiveness within parties regarding the overall strategic posture, political managers need to be aware......Purpose – This paper investigates the relationship between the strategic postures and political market orientation profile of two Danish parties. Profile stability at the organisational level is used as a control variable. Design/methodology/approach – The strategic political postures of two Danish...

  6. Party and candidate websites: A comparative explanatory analysis

    NARCIS (Netherlands)

    Kruikemeier, S.; Aparaschivei, A.P.; Boomgaarden, H.G.; van Noort, G.; Vliegenthart, R.

    2015-01-01

    This study provides a systematic investigation of party and candidate websites across five countries. It examines three prominent features of current online political communication (interactivity, political personalization, and mobilization). Furthermore it assesses to what extent country, party, an

  7. Asynchronous Multi-Party Computation with Quadratic Communication

    DEFF Research Database (Denmark)

    Hirt, Martin; Nielsen, Jesper Buus; Przydatek, Bartosz

    2008-01-01

    We present an efficient protocol for secure multi-party computation in the asynchronous model with optimal resilience. For n parties, up to t computed with communication complexity O(cn^2k) bits, which...

  8. Public funding of political parties when campaigns are informative

    DEFF Research Database (Denmark)

    Ortín, Ignacio Ortuño; Schultz, Christian

    ’s dependence on vote shares induces fur- ther moderation and improves welfare. If parties are asymmetric, vote share dependent public funding bene…ts the large party and makes it moderate its candidate, while the smaller party reacts by choosing a more extremist candidate. On balance, however, if the parties...... are not too asymmetric, an increase in vote share dependent funding improves welfare and increases the likelihood that a moderate candidate wins the election...

  9. Ombuds’ corner: the third party, the institution

    CERN Multimedia

    Vincent Vuillemin

    2012-01-01

    “The Ombuds shall provide confidential assistance for the informal resolution of interpersonal issues, in the interests of the good functioning of CERN.”*   In any institution, conflicts are inevitable. They can, however, offer an opportunity for a positive resolution. Relationships in a workplace are generally better and stronger between people who have been able to reach a positive resolution of their disagreement, than they are between people who get along moderately well. However, in disputes involving two antagonistic parties, people often forget that there is actually a third party behind the scenes: the institution. It is sometimes surprising to see that even people holding management responsibilities or hierarchical positions in projects fight each other, and completely forget that they are accountable for the good functioning of CERN. Commitment and professionalism are part of the five priority values of CERN, as expressed in its Code of Conduct: “Promot...

  10. Multi-Party Pseudo-Telepathy

    CERN Document Server

    Brassard, G; Tapp, A; Brassard, Gilles; Broadbent, Anne; Tapp, Alain

    2003-01-01

    Quantum entanglement, perhaps the most non-classical manifestation of quantum information theory, cannot be used to transmit information between remote parties. Yet, it can be used to reduce the amount of communication required to process a variety of distributed computational tasks. We speak of pseudo-telepathy when quantum entanglement serves to eliminate the classical need to communicate. In earlier examples of pseudo-telepathy, classical protocols could succeed with high probability unless the inputs were very large. Here we present a simple multi-party distributed problem for which the inputs and outputs consist of a single bit per player, and we present a perfect quantum protocol for it. We prove that no classical protocol can succeed with a probability that differs from 1/2 by more than a fraction that is exponentially small in the number of players. This could be used to circumvent the detection loophole in experimental tests of nonlocality.

  11. To Defer or To Stand Up? How Offender Formidability Affects Third Party Moral Outrage

    DEFF Research Database (Denmark)

    Jensen, Niels Holm; Petersen, Michael Bang

    2011-01-01

    According to models of animal behavior, the relative formidability of conspecifics determines the utility of deferring versus aggressing in situations of conflict. Here we apply and extend these models by investigating how the formidability of exploiters shapes third party moral outrage in humans...

  12. General polygamy inequality of multi-party quantum entanglement

    CERN Document Server

    Kim, Jeong San

    2012-01-01

    Using entanglement of assistance, we establish a general polygamy inequality of multi-party entanglement in arbitrary dimensional quantum systems. For multi-party closed quantum systems, we relate our result with the monogamy of entanglement to show that the entropy of entanglement is an universal entanglement measure that bounds both monogamy and polygamy of multi-party quantum entanglement.

  13. 39 CFR 955.20 - Unexcused absence of a party.

    Science.gov (United States)

    2010-07-01

    ... 39 Postal Service 1 2010-07-01 2010-07-01 false Unexcused absence of a party. 955.20 Section 955.20 Postal Service UNITED STATES POSTAL SERVICE PROCEDURES RULES OF PRACTICE BEFORE THE POSTAL SERVICE BOARD OF CONTRACT APPEALS § 955.20 Unexcused absence of a party. The unexcused absence of a party at the...

  14. 41 CFR 102-78.30 - Who are consulting parties?

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Who are consulting... Preservation § 102-78.30 Who are consulting parties? As more particularly described in 36 CFR 800.2(c), consulting parties are those parties having consultative roles in the Section 106 process (i.e., Section 106...

  15. 48 CFR 552.270-3 - Parties To Execute Lease.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Parties To Execute Lease... Parties To Execute Lease. As prescribed in 570.602, insert the following provision: Parties To Execute Lease (SEP 1999) (a) If the lease is executed by an attorney, agent, or trustee on behalf of the...

  16. The proscription of parties and the problem with 'militant democracy'

    DEFF Research Database (Denmark)

    Bourne, Angela

    2012-01-01

    In 2003, Spain banned the political party Herri Batasuna and its successors Euskal Herritarrok and Batasuna for integration in the Basque separatist terrorist group Euskadi Ta Askatasuna. Since then, eleven other parties or electoral groupings deemed successors to the illegal parties have also been...

  17. A Practical Guide to Preventing and Dispersing Underage Drinking Parties

    Science.gov (United States)

    Pacific Institute for Research and Evaluation, 2011

    2011-01-01

    This guide describes the role of enforcement and community organizations or groups in preventing underage drinking parties and how to safely disperse them. It describes the problem of underage drinking, in general, and youth-drinking parties in particular. It provides step-by-step information on how to address underage drinking parties and how to…

  18. The Dark Side of Altruistic Third-Party Punishment

    Science.gov (United States)

    Leibbrandt, Andreas; Lopez-Perez, Raul

    2011-01-01

    This article experimentally studies punishment from unaffected third parties in ten different games. The authors show that third-party punishment exhibits several features that are arguably undesirable. First, third parties punish strongly a decider if she chooses a socially efficient or a Pareto efficient allocation and becomes the richest party…

  19. Social Networks and Political Parties in Chile

    Directory of Open Access Journals (Sweden)

    Adler Lomnitz, Larissa

    2002-09-01

    Full Text Available This paper describes the origin and evolution of two Chilean political parties (the Radical Party and the Christian Democrat Party through the analysis of the social networks that originated and composed them. The aim of this study is to propose a model of national political cultures on the basis of the structure of social networks related to power and of the symbol system, which legitimizes it. The structure of social networks, horizontal and vertical, are based on reciprocal or redistributive forms of exchange, on what is being exchanged and on the articulation between networks. In every society there are symmetrical and asymmetrical exchanges, which produce horizontal and vertical networks. These networks interact among themselves to form the social fabric. The dominance of some over others and how they combine, delineate the character of the political culture (authoritarian vs. egalitarian. Chile is a multiparty country within which there are cohorts of horizontal groups of friends, who informally exercise a central control over their members and create invisible boundaries setting them apart from others, in which leadership is under constrains. The result is both a strong presidential system based on an almost fanatic legitimacy, combined with factionalism and a strong parliamentary system.

  20. Inhibition of Re Du Ning Injection on Enzyme Activities of Rat Liver Microsomes Using Cocktail Method

    Institute of Scientific and Technical Information of China (English)

    Xiao-qian Xu; Ting Geng; She-bing Zhang; Dan-yu Kang; Yan-jing Li; Gang Ding; Wen-zhe Huang; Zhen-zhong Wang; Wei Xiao

    2016-01-01

    Objective Re Du Ning Injection(RDN), a Chinese materia medica injection, is made from the extracts of Lonicerae Japonicae Flos, Gardeniae Fructus, and Artemisiae Annuae Herba. Since last decade, RDN has been widely used in China for the treatment of viral infection, fever, and inflammation. To assess the potential interacting of RDN with co-administered drugs, the inhibitory effects of RDN on the enzyme activities(CYP1A1, CYP1A2, CYP2C11, CYP2D1, and CYP3A1/2) of rat liver microsomes were investigated by a cocktail method. Methods A sensitive and specific LC-MS method capable of simultaneous quantification of five metabolites in rat liver microsomes was developed and validated. Then RDN(0.625%–1.0%) was incubated with rat liver microsomes and specific substrates. The enzyme activities were expressed as the formation rate of the specific metabolites of the substrates(pmol·mg·protein-1·min-1). Results RDN competitively inhibited the activities of CYP1A2 and CYP2C11, with inhibition constant(Ki) values determined to be 0.18% and 0.63%, respectively. RDN exhibited the mixed inhibition on the activity of CYP2D1, with a Ki value of 0.15%. The activities of CYP1A1 and CYP3A1/2 were not markedly inhibited even by 1.0% RDN. Conclusion RDN could inhibit the rat enzyme activities of CYP1A2, 2C11, and 2D1 in vitro with different inhibition modes, which is worthy of promoting safety and efficacy of RDN.

  1. The Boston Marathon Bombers: the Lethal Cocktail that Turned Troubled Youth to Terrorism

    Directory of Open Access Journals (Sweden)

    Anne Speckhard

    2013-06-01

    Full Text Available The Tsarnaev brothers came from the war-torn Chechen diaspora and found asylum in the United States. The elder brother,Tamerlan, carried actual memories of fleeing from the 1994-96 Chechen war. He also lived in Dagestan for a year at the time when the Chechen warlord Basayev was engaging in a campaign of more than thirty suicide attacks, including mass hostage takings. When Tamerlan made it to the United States, he tried to assimilate but failed to make it. The family struggled hard to make a living in the U.S.; but in the end the parents divorced and the father returned to Dagestan. Dzhokhar, the younger brother, made it into an American university but Tamerlan did not succeed in higher education, trying to pursue a boxing career instead. When his immigration status precluded a boxing career, he became disillusioned. Meanwhile his mother, concerned about his descent into smoking marijuana and drinking alcohol, arranged an Islamic tutor for him. The ingredients for the lethal cocktail of terrorism came together when Tamerlan's individual vulnerabilities made him seek online contacts via the Internet and brought him in touch with offline mentors, militants and extremists who glorified Al-Qaeda’s global jihad. Through these channels he became receptive to the terrorist ideology, found a jihadist group with global reach to align with, received technical instructions from the Inspire magazine and found enough support for executing the Boston marathon attack, pulling his younger brother along and dying in the process as he had expected.

  2. Inter-party agenda-setting in the Belgian parliament: the role of party characteristics and competition

    NARCIS (Netherlands)

    Vliegenthart, R.; Walgrave, S.; Meppelink, C.

    2011-01-01

    In this article we explore the inter-party agenda-setting dynamics in the Belgian parliament during the period 1993-2000 and investigate whether and when parties respond to the attention paid to issues by other parties in parliament. We rely on an elaborate coding of parliamentary questions and inte

  3. Inter-party agenda-setting in the Belgian parliament: the role of party characteristics and competition

    NARCIS (Netherlands)

    R. Vliegenthart; S. Walgrave; C. Meppelink

    2010-01-01

    In this article we explore the inter-party agenda-setting dynamics in the Belgian parliament during the period 1993-2000 and investigate whether and when parties respond to the attention paid to issues by other parties in parliament. We rely on an elaborate coding of parliamentary questions and inte

  4. Characterization of vB_SauM-fRuSau02, a Twort-Like Bacteriophage Isolated from a Therapeutic Phage Cocktail

    Directory of Open Access Journals (Sweden)

    Katarzyna Leskinen

    2017-09-01

    Full Text Available Staphylococcus aureus is a commensal and pathogenic bacterium that causes infections in humans and animals. It is a major cause of nosocomial infections worldwide. Due to increasing prevalence of multidrug resistance, alternative methods to eradicate the pathogen are necessary. In this respect, polyvalent staphylococcal myoviruses have been demonstrated to be excellent candidates for phage therapy. Here we present the characterization of the bacteriophage vB_SauM-fRuSau02 (fRuSau02 that was isolated from a commercial Staphylococcus bacteriophage cocktail produced by Microgen (Moscow, Russia. The genomic analysis revealed that fRuSau02 is very closely related to the phage MSA6, and possesses a large genome (148,464 bp, with typical modular organization and a low G+C (30.22% content. It can therefore be classified as a new virus among the genus Twortlikevirus. The genome contains 236 predicted genes, 4 of which were interrupted by insertion sequences. Altogether, 78 different structural and virion-associated proteins were identified from purified phage particles by liquid chromatography-tandem mass spectrometry (LC-MS/MS. The host range of fRuSau02 was tested with 135 strains, including 51 and 54 Staphylococcus aureus isolates from humans and pigs, respectively, and 30 coagulase-negative Staphylococcus strains of human origin. All clinical S. aureus strains were at least moderately sensitive to the phage, while only 39% of the pig strains were infected. Also, some strains of Staphylococcus intermedius, Staphylococcus lugdunensis, Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus saprophyticus and Staphylococcus pseudointer were sensitive. We conclude that fRuSau02, a phage therapy agent in Russia, can serve as an alternative to antibiotic therapy against S. aureus.

  5. The lives of others: Third parties' responses to others' injustice.

    Science.gov (United States)

    O'Reilly, Jane; Aquino, Karl; Skarlicki, Daniel

    2016-02-01

    This research takes a moral perspective to studying third parties' reactions to injustice as a function of their moral identity. Drawing from theories of deontic justice, moral intuition, moral heuristics, and moral identity, we develop and test a model of the moral underpinnings of third parties' reactions to injustice. First, we compare third parties' responses with interpersonal, distributive, and procedural justice violations. We hypothesize that third parties are more likely to intuit that interpersonal justice violations are morally wrong, compared with distributive and procedural justice violations. As a result, third parties are more likely to experience stronger moral anger and punish violators in response to interpersonal transgressions compared with distributive and procedural justice transgressions. Second, we test the proposition that third parties with a strong moral identity will react more strongly to justice violations than third parties with a comparatively weak moral identity. Results from 3 studies support these predictions. (c) 2016 APA, all rights reserved).

  6. Environmental risk assessment of three selective serotonin reuptake inhibitors in the aquatic environment: a case study including a cocktail scenario.

    Science.gov (United States)

    Styrishave, Bjarne; Halling-Sørensen, Bent; Ingerslev, Flemming

    2011-01-01

    We present an environmental risk assessment of three selective serotonin reuptake inhibitors (SSRIs; citalopram, sertraline, and fluoxetine) in the aquatic environment based on two case scenarios. Abiotic and biotic degradation experiments and sorption estimates were used to predict environmental concentrations of three SSRIs from the wastewater of two psychiatric hospitals, the primary sector, and wastewater entering and leaving wastewater treatment plants (WWTPs). Assuming a sewage treatment retention time of 8 h, abiotic degradation was low, for all three SSRIs inhibitors, ranging between 0 and 2% for hydrolysis and 0 and 6% for photolysis. The biodegradation was also slow, ranging from 0 to 3% within an 8-h period. In untreated sewage, citalopram (CIT) and sertraline (SER) concentrations may be high enough to exert effects on the aquatic biota (CIT: 0.19-10.3 µg/L; SER: 0.14-17.1 µg/L). Removal of the pharmaceuticals is due primarily to sorption in the WWTP. Sertraline was estimated to have the highest concentrations in the sewage effluents, 4.4 and 19.9 ng/L for the two cases, respectively. In treated wastewater, individual SSRI concentrations are probably too low to exert effects on biota. By using concentration addition, a cocktail exposure scenario was estimated. The predicted concentration in the biota calculated from the cocktail effect was 0.05 and 0.16 nmol/g for the two cases, respectively, and SER was found to give the highest contribution to this cocktail effect. The results indicate that the concentrations in the wastewater effluents are one to two orders of magnitude lower than the concentrations likely to cause an effect in the aquatic biota.

  7. COMPARATIVE EFFICACY (SEDATIVE AND ANAESTHETIC OF DETOMIDINE, KETAMINE AND DETOMIDINE-KETAMINE COCKTAIL IN PIGEONS (COLUMBA LIVIA

    Directory of Open Access Journals (Sweden)

    UZMA F. DURRANI, M. ARIF KHAN1 AND S. SALEEM AHMAD

    2008-07-01

    Full Text Available A study was conducted to compare the synergistic efficacy of detomidine, ketamine and their cocktail in pigeons (Columba livia. For this study, 15 adult and healthy pigeons were divided into three equal groups A, B and C. Birds of groups A and B were intramuscularly administered detomidine and ketamine @ 1.4 and 60 mg/kg b. wt., respectively. Pigeons of group C received detomidine + Ketamine cocktail @ 0.7 and 30 mg/kg b. wt. Induction of sedation and anaesthesia was smooth in all groups. Mean duration of induction was 11.1 + 2.03, 11.0 + 1.49 and 1.6 + 0.48 minutes in groups A, B, C, respectively. In groups A and B, smooth but light sedation and anaesthesia were observed accompanied by superficial analgesia, while in group C, birds showed deep anaesthesia alongwith deep analgesia. Birds in groups A and C elicited hypothermia, respiratory depression and bradycardia till complete recovery, while group B showed hyperthermia and tachycardia with rapid respiration. In group A, sedation persisted for 54.2 + 21.82 minutes and mean recovery period was 49.9 + 5.91 minutes, while groups B and C had anaesthesia for 47.7 + 8.06 and 103.5 + 27.52 minutes, and recovery periods were 52.6 + 9.64 and 61.3 + 17.26 minutes, respectively. Recovery was rough in group B and smooth in groups A and C. It was concluded that in pigeons, detomidine (alone is safe for handling and for least painful procedures, while detomidine-ketamine cocktail is safe as intramuscular anaesthetic for major surgical procedures. However, ketamine is not a good anaesthetic to be used alone in pigeons.

  8. Political Market Orientation and Strategic Party Postures in Danish Political Parties

    DEFF Research Database (Denmark)

    Ormrod, Robert P.; Henneberg, Stephan

    2011-01-01

    in the political sphere. More specifically it empirically links political market orientation as an issue of political marketing implementation on the one hand, and strategic postures of parties as a strategic issue on the other, following a configuration theory logic. Research limitations......Purpose – This paper investigates the relationship between the strategic postures and political market orientation profile of two Danish parties. Profile stability at the organisational level is used as a control variable. Design/methodology/approach – The strategic political postures of two Danish...... Ideologist. Thus, Party A exhibits a mismatch between strategic orientation and implemented organisational profile, based on configuration theory. The results were generally stable across political activity levels. Originality/value – The study contributes to understanding the concept of market orientation...

  9. Prescribing Democracy? Party Proscription and Party System Stability in Turkey, Germany and Spain

    DEFF Research Database (Denmark)

    Bourne, Angela; Casals Bertoa, Fernando

    2017-01-01

    of survey data when available, we find that the banning of a relevant political party not only increases volatility and reduces fragmentation, but also alter the existing structure of competition at the time of government formation. Likewise, our empirical analysis also suggests a number of alternative......, parliamentary and governmental. In particular, we examine the impact of party proscription on electoral volatility, fragmentation and closure in three different countries: Turkey, Germany and Spain. Using examples both at national and regional (e.g. Basque Country, Navarre, Saxony) level, and making use...

  10. So Close But So Far: Voting Propensity and Party Choice for Left-�Wing Parties

    DEFF Research Database (Denmark)

    Bochsler, Daniel; Sciarini, Pascal

    2010-01-01

    While the bulk of the literature focuses on the vote for parties from different blocs, the purpose of our article is to study the vote for two parties that are ideologically very close to each other: The Social Democrats and the Greens in Switzerland. To that end, we develop a two-step model, where...... on the Left–Right scale. With regard to the second, choice stage of the electoral process we hypothesize about the factors that may account for the varying ability of the two par- ties to convert potential voters into real voters. Our empirical tests provide encourag- ing support for our hypotheses regarding...

  11. The Feminist Consciousness in The Garden Party

    Institute of Scientific and Technical Information of China (English)

    ZHAO Song-zhi

    2016-01-01

    Feminist movement reaches its climax in the first two decades of the 20th century. There are many female novelists in that time and Mansfield is one of them. Most of her novels are full of feminist consciousness, and The Garden Party is a special one. The characters in the story are different from other story. The author describes how the society and its spokesman Mrs. Sher-idan restrain the nature of Laura in detail. The author uses Laura to hint the current situation of women.

  12. Efficient secure two-party protocols

    CERN Document Server

    Hazay, Carmit

    2010-01-01

    The authors present a comprehensive study of efficient protocols and techniques for secure two-party computation -- both general constructions that can be used to securely compute any functionality, and protocols for specific problems of interest. The book focuses on techniques for constructing efficient protocols and proving them secure. In addition, the authors study different definitional paradigms and compare the efficiency of protocols achieved under these different definitions.The book opens with a general introduction to secure computation and then presents definitions of security for a

  13. An RNAi in silico approach to find an optimal shRNA cocktail against HIV-1.

    Science.gov (United States)

    Méndez-Ortega, María C; Restrepo, Silvia; Rodríguez-R, Luis M; Pérez, Iván; Mendoza, Juan C; Martínez, Andrés P; Sierra, Roberto; Rey-Benito, Gloria J

    2010-12-20

    HIV-1 can be inhibited by RNA interference in vitro through the expression of short hairpin RNAs (shRNAs) that target conserved genome sequences. In silico shRNA design for HIV has lacked a detailed study of virus variability constituting a possible breaking point in a clinical setting. We designed shRNAs against HIV-1 considering the variability observed in naïve and drug-resistant isolates available at public databases. A Bioperl-based algorithm was developed to automatically scan multiple sequence alignments of HIV, while evaluating the possibility of identifying dominant and subdominant viral variants that could be used as efficient silencing molecules. Student t-test and Bonferroni Dunn correction test were used to assess statistical significance of our findings. Our in silico approach identified the most common viral variants within highly conserved genome regions, with a calculated free energy of ≥ -6.6 kcal/mol. This is crucial for strand loading to RISC complex and for a predicted silencing efficiency score, which could be used in combination for achieving over 90% silencing. Resistant and naïve isolate variability revealed that the most frequent shRNA per region targets a maximum of 85% of viral sequences. Adding more divergent sequences maintained this percentage. Specific sequence features that have been found to be related with higher silencing efficiency were hardly accomplished in conserved regions, even when lower entropy values correlated with better scores. We identified a conserved region among most HIV-1 genomes, which meets as many sequence features for efficient silencing. HIV-1 variability is an obstacle to achieving absolute silencing using shRNAs designed against a consensus sequence, mainly because there are many functional viral variants. Our shRNA cocktail could be truly effective at silencing dominant and subdominant naïve viral variants. Additionally, resistant isolates might be targeted under specific antiretroviral selective

  14. An RNAi in silico approach to find an optimal shRNA cocktail against HIV-1

    Directory of Open Access Journals (Sweden)

    Mendoza Juan C

    2010-12-01

    Full Text Available Abstract Background HIV-1 can be inhibited by RNA interference in vitro through the expression of short hairpin RNAs (shRNAs that target conserved genome sequences. In silico shRNA design for HIV has lacked a detailed study of virus variability constituting a possible breaking point in a clinical setting. We designed shRNAs against HIV-1 considering the variability observed in naïve and drug-resistant isolates available at public databases. Methods A Bioperl-based algorithm was developed to automatically scan multiple sequence alignments of HIV, while evaluating the possibility of identifying dominant and subdominant viral variants that could be used as efficient silencing molecules. Student t-test and Bonferroni Dunn correction test were used to assess statistical significance of our findings. Results Our in silico approach identified the most common viral variants within highly conserved genome regions, with a calculated free energy of ≥ -6.6 kcal/mol. This is crucial for strand loading to RISC complex and for a predicted silencing efficiency score, which could be used in combination for achieving over 90% silencing. Resistant and naïve isolate variability revealed that the most frequent shRNA per region targets a maximum of 85% of viral sequences. Adding more divergent sequences maintained this percentage. Specific sequence features that have been found to be related with higher silencing efficiency were hardly accomplished in conserved regions, even when lower entropy values correlated with better scores. We identified a conserved region among most HIV-1 genomes, which meets as many sequence features for efficient silencing. Conclusions HIV-1 variability is an obstacle to achieving absolute silencing using shRNAs designed against a consensus sequence, mainly because there are many functional viral variants. Our shRNA cocktail could be truly effective at silencing dominant and subdominant naïve viral variants. Additionally, resistant

  15. Scintillating Cocktail Mixtures and the Role of Water on the Optophysical Properties.

    Energy Technology Data Exchange (ETDEWEB)

    Cordaro, Joseph Gabriel [Sandia National Lab. (SNL-CA), Livermore, CA (United States); Feng, Patrick L. [Sandia National Lab. (SNL-CA), Livermore, CA (United States); Mengesha, Wondwosen [Sandia National Lab. (SNL-CA), Livermore, CA (United States); Murtagh, Dustin [Sandia National Lab. (SNL-CA), Livermore, CA (United States); Anstey, Mitchell [Sandia National Lab. (SNL-CA), Livermore, CA (United States)

    2015-10-01

    Two types of water - containing liquid scinti llation mixtures were prepared in the present work. In the first, m ixtures of 2 - phenylethanol, water, diphenyl phosphate, sodium phenyl phosphate dibasic dihydrate, and the dye 2,5 - diphenyloxazole (PPO) have been investigated as liquid scintillators. In th e second system, nonionic and mixed surfactant systems were investigated in conjunction with water and small amounts of toluene. In both cases, increasing amounts of water led to reductions in the scintillation light yield. Understanding what factors contr ibute to this phenomenon is the focus of this report. Changes in the solution microphase structure, diminishing aromatic content of the cocktail mixtures, and inefficient energy transfer to the dye a ppear to be responsible for the decreased light yield as more water is added . In the 2 - phenylethanol system, the observed experimental results are consistent with the formation of a bicontinuous microemulsion at higher water concentrations, which incorporates PPO and shields it from the quenching effects of the increasing polar matrix. Evidence for this proposed phase chan ge c ome s from light scattering data, photo - and x - ray luminescence measurements, and optical transparency measurements . In the surfactant - based system, the quenching effect of water was found to be less than both commercially - available dioxane - naphthalene mixtures used for scintillation counting as well as the 2 - phenylethanol mixtures described above. The effect of different surfactant mixtures and concentrations were studied, revealing a benefic ial effect upon the scintillation light yield for mixed surfactant mixtures. These results are interpreted in the context of r eactive radical species formation following water ionization , which leads to light - yield quenching in aqueous systems . The presenc e of surfactant(s) in these mixtures enables the formation of organic - rich regions that are spatially separated from the

  16. Party Secretaries in Chinese Higher Education Institutions: What Roles Do They Play?

    Science.gov (United States)

    Jiang, Hua; Li, Xiaobin

    2016-01-01

    The Chinese political party in power is the Communist Party. In higher education institutions the Party secretary is a ubiquitous presence. The purpose of this study was to answer the question, "What roles do Party secretaries play?" The Party committee headed by the Party secretary in an institution is supposed to lead the institution.…

  17. From the Two-Party to the Dominant-Party System in Mozambique, 1994-2012: Framing Frelimo Party Dominance in Context

    NARCIS (Netherlands)

    A.A. Nuvunga

    2014-01-01

    markdownabstract__Abstract__ This thesis examines the abrupt end of Mozambique’s vibrant two-party system, which, by the middle of the past decade, had developed into a dominant- party system, instead of institutionalizing, as was the case in other African third wave democracies. While, from the se

  18. Intensive sex partying amongst gay men in Sydney.

    Science.gov (United States)

    Hurley, Michael; Prestage, Garrett

    2009-08-01

    Intensive sex partying is a framework developed to analyse specific frequent behaviours amongst a small minority of gay men in Sydney, Australia. The behaviours included a higher frequency of dance party attendance, more frequent sex, more anal sex, multiple sex partners, more unprotected anal intercourse with casual partners and more frequent drug taking. These occur at a contextual intersection between a sub-group of sexually adventurous gay men and 'party boys'. The men appear to be involved in both high-risk, adventurous sex practices and a specific form of partying distinguishable from dance partying and 'clubbing'. Sex partying occurs on multiple sites (domestic spaces; within dance parties; sex parties; sex-on-premises venues) and appears to be geared to the maximisation of sexual pleasure. Intensive sex partying describes this coincidence of factors and locates them in relation to the multiple pleasures offered by sex partying. It emphasises the importance of 'intensity' in order to understand better the relations between sex, drug use, pleasure, care and risk in some gay men's lives.

  19. [Third-party funding and industrial sponsoring].

    Science.gov (United States)

    Bock, R-W

    2003-11-01

    Due to sensational publications, media-effective proceedings and not least due to personal involvement in many cases the medical profession became aware of the potential criminal significance of third-party funding and industrial sponsoring in hospitals. It started with the so-called Heart-Valve-Affair ("Herzklappenskandal") in 1994. Since then, insecurity prevails which was further nourished by a new series of legal proceedings in spring 2002. Industrial sponsoring has been practised in hospitals for a long time. Research work at universities and colleges would hardly be possible without third-party funding, which is accepted de lege lata according to section sign 25 of the German Hochschulrahmengesetz (framework law on universities and colleges). In view of the course of action of the criminal prosecution authorities the question came up which precautions must be taken with regard to grants by the medical-pharmaceutical industry to avoid consequences from the point of view of criminal law.[nl]There are various facts and circumstances in relation with these benefits, such as providing financing of educational training, payments related to the participation in congresses, speaker's fees, consultancy agreements, clinical tests and application evaluation, provision of equipment and staff as well as providing financing thereof, donations to medical facilities and so-called Fördervereine (supporting associations), benefits for research projects, etc.

  20. LES PARTIES DU CONTRAT DE CONCESSION

    Directory of Open Access Journals (Sweden)

    Octavian OLARU

    2008-09-01

    Full Text Available The essential feature of the administrative contract, which is part of the concession contract, differentiates from the civil contract with at least one of the topics of administrative contract that is a physical person of public law. Thus, if the subject of civil contract can be any physical or juridical person that has full the capacity to exercise, then, in the case of the administrative contract, at least one subject must have the special “quality”, namely to be a public authority or a moral person of public law. If one of the contracting parties is a public figure and the other is a person of private law, the contract may be administrative. “This condition is necessary, but not sufficient”. By the definition given to concession in article 1 line 1 of the Law on concessions, the legislator determines not only the name of the contracting parties, which formed the juridical report (the conceded, the concessionaire but also the subject concession contract, which makes it different from other administrative contracts. Moreover, in the article 4 of the cited law, it is determined the scope of the public authorities, juridical persons of public law that may have the quality of conceded, while article 5 provides the scope of persons who may have the quality of concessionaire.

  1. Clivages partisans et partis politiques en Libye

    Directory of Open Access Journals (Sweden)

    Moncef Djaziri

    2011-12-01

    Full Text Available La société libyenne traverse une période de grands changements socio-économiques qui annoncent à moyen terme d’importants changements politiques. Le bon déroulement de la transition à laquelle la Libye devra faire face, dépend en partie du rôle que pourront y jouer les partis politiques. Cet article est consacré à l’analyse des clivages politiques et partisans en Libye. Après un bref aperçu théorique et un rappel des premières expériences partisanes pendant la période coloniale et au cours des premières années qui ont suivi le prise du pouvoir par Kadhafi, ce texte porte tout particulièrement sur la structuration des oppositions politiques contempo­raines avec leurs ressemblances et différences. Enfin, et s’agissant de la transition, l’hypothèse d’une dynastisation du pouvoir en Libye, évoquée à la fin de l’article, paraît pertinente eu égard à plusieurs facteurs. Une telle transition impliquera la prise en compte des clivages existants et devra s’appuyer sur les organisations partisanes dans ce pays.

  2. Picturing the Party: Instagram and Party Campaigning in the 2014 Swedish Elections

    Directory of Open Access Journals (Sweden)

    Kirill Filimonov

    2016-08-01

    Full Text Available This article explores Swedish parties’ activities on Instagram during the 2014 elections. Understanding party campaign communication as highly strategic, that is, communication to persuade and mobilize voters in order to win the elections, we ask whether Instagram was used to (1 broadcast campaign messages, (2 mobilize supporters, (3 manage the party’s image, and (4 amplify and complement other campaign material (i.e., hybrid campaign use. With this study, we follow previous studies on the use of digital communication platforms in the hands of campaigning political actors, but we direct our attention to a new platform. We conducted a content analysis of 220 party postings on Instagram, collected during the hot phase of the campaign. The result shows that the platform was mainly used for broadcasting rather than for mobilization. The image the parties were presenting leaned toward personalization with a strong presence of top candidates in their postings. Top candidates were primarily displayed in a political/professional context. Finally, half of the analyzed postings showed signs of hybridized campaign practices. The presented findings give a first glimpse on how political parties use and perform on Instagram.

  3. Party politics as usual? The role of political parties in EU legislative decision-making

    NARCIS (Netherlands)

    Lindberg, Bjorn; Rasmussen, Anne; Warntjen, Andreas

    2008-01-01

    Scholars have raised doubts about the ability of political parties to fulfil their traditional role as 'transmission belts' between citizens and legislators in the EU. We discuss how the different institutional environment of the EU affects the assumptions and predictions of theories of political pa

  4. Immune response of calves immunized with cocktail of DNA vaccine encoding complexed outer membrane proteins from Anaplasma marginale

    Directory of Open Access Journals (Sweden)

    Adriana Letícia Mendes Coelho

    2014-02-01

    Full Text Available In the present study, we evaluated a cocktail of recombinant plasmids encoding the major surface proteins (rMSP1a, rMSP1b, rMSP4, rMSP5, VirB9 and VirB10 to improve the humoral and cellular immune response triggered in calves. Ten calves were divided in three groups: Four calves were inoculated with the plasmids/rMSPs (G1; 2 calves were inoculated with empty vector (G2; and 4 calves received saline (G3. Three inoculations were administered at 21-day intervals. In G1, the calves showed significant increases in total IgG level 21 days after the second inoculation, compared to the control group (p<0,05%, and G1 calves remained above the cut-off value 28 days after the third inoculation (p<0,05%. The post-immunized sera from calves in G1 reacted specifically for each of the rMSPs used. Additionally, ELISA detected specific IgG for VirB9 and VirB10. In conclusion, the cocktail of recombinant plasmids induced antigen-specific seroconversion in calves. Therefore, additional testing to explore the protection induced by recombinant plasmids, alone or in conjunction with proteins previously identified as subdominant epitopes, is warranted.

  5. Co-cultivation of Aspergillus nidulans recombinant strains produces an enzymatic cocktail as alternative to alkaline sugarcane bagasse pretreatment

    Directory of Open Access Journals (Sweden)

    Matheus Sanita Lima

    2016-04-01

    Full Text Available Plant materials represent a strategic energy source because they can give rise to sustainable biofuels through the fermentation of their carbohydrates. A clear example of a plant-derived biofuel resource is the sugar cane bagasse exhibiting 60 % - 80 % of fermentable sugars in its composition. However, the current methods of plant bioconversion employ severe and harmful chemical/physical pretreatments raising biofuel cost production and environmental degradation. Replacing these methods with co-cultivated enzymatic cocktails is an alternative. Here we propose a pretreatment for sugarcane bagasse using a multi-enzymatic cocktail from the co-cultivation of four Aspergillus nidulans recombinant strains. The co-cultivation resulted in the simultaneous production of GH51 arabinofuranosidase (AbfA, GH11 endo-1,4-xylanase (XlnA, GH43 endo-1,5-arabinanase (AbnA and GH12 xyloglucan specific endo-β-1,4-glucanase (XegA. This core set of recombinant enzymes was more efficient than the alternative alkaline method in maintaining the cellulose integrity and exposing this cellulose to the following saccharification process. Thermogravimetric and differential thermal analysis revealed residual byproducts on the alkali pretreated biomass, which were not found in the enzymatic pretreatment. Therefore, the enzymatic pretreatment was residue-free and seemed to be more efficient than the applied alkaline method, which makes it suitable for bioethanol production.

  6. Co-cultivation of Aspergillus nidulans Recombinant Strains Produces an Enzymatic Cocktail as Alternative to Alkaline Sugarcane Bagasse Pretreatment

    Science.gov (United States)

    Lima, Matheus S.; Damasio, André R. de L.; Crnkovic, Paula M.; Pinto, Marcelo R.; da Silva, Ana M.; da Silva, Jean C. R.; Segato, Fernando; de Lucas, Rosymar C.; Jorge, João A.; Polizeli, Maria de L. T. de M.

    2016-01-01

    Plant materials represent a strategic energy source because they can give rise to sustainable biofuels through the fermentation of their carbohydrates. A clear example of a plant-derived biofuel resource is the sugar cane bagasse exhibiting 60–80% of fermentable sugars in its composition. However, the current methods of plant bioconversion employ severe and harmful chemical/physical pretreatments raising biofuel cost production and environmental degradation. Replacing these methods with co-cultivated enzymatic cocktails is an alternative. Here we propose a pretreatment for sugarcane bagasse using a multi-enzymatic cocktail from the co-cultivation of four Aspergillus nidulans recombinant strains. The co-cultivation resulted in the simultaneous production of GH51 arabinofuranosidase (AbfA), GH11 endo-1,4-xylanase (XlnA), GH43 endo-1,5-arabinanase (AbnA) and GH12 xyloglucan specific endo-β-1,4-glucanase (XegA). This core set of recombinant enzymes was more efficient than the alternative alkaline method in maintaining the cellulose integrity and exposing this cellulose to the following saccharification process. Thermogravimetric and differential thermal analysis revealed residual byproducts on the alkali pretreated biomass, which were not found in the enzymatic pretreatment. Therefore, the enzymatic pretreatment was residue-free and seemed to be more efficient than the applied alkaline method, which makes it suitable for bioethanol production. PMID:27199917

  7. Use of a cocktail of spin traps for fingerprinting large range of free radicals in biological systems.

    Science.gov (United States)

    Marchand, Valérie; Charlier, Nicolas; Verrax, Julien; Buc-Calderon, Pedro; Levêque, Philippe; Gallez, Bernard

    2017-01-01

    It is well established that the formation of radical species centered on various atoms is involved in the mechanism leading to the development of several diseases or to the appearance of deleterious effects of toxic molecules. The detection of free radical is possible using Electron Paramagnetic Resonance (EPR) spectroscopy and the spin trapping technique. The classical EPR spin-trapping technique can be considered as a "hypothesis-driven" approach because it requires an a priori assumption regarding the nature of the free radical in order to select the most appropriate spin-trap. We here describe a "data-driven" approach using EPR and a cocktail of spin-traps. The rationale for using this cocktail was that it would cover a wide range of biologically relevant free radicals and have a large range of hydrophilicity and lipophilicity in order to trap free radicals produced in different cellular compartments. As a proof-of-concept, we validated the ability of the system to measure a large variety of free radicals (O-, N-, C-, or S- centered) in well characterized conditions, and we illustrated the ability of the technique to unambiguously detect free radical production in cells exposed to chemicals known to be radical-mediated toxic agents.

  8. Prospecting Agro-waste Cocktail: Supplementation for Cellulase Production by a Newly Isolated Thermophilic B. licheniformis 2D55.

    Science.gov (United States)

    Kazeem, Muinat Olanike; Shah, Umi Kalsom Md; Baharuddin, Azhari Samsu; AbdulRahman, Nor' Aini

    2017-02-07

    Bacteria isolated from thermophilic environment that can produce cellulase as well as utilise agro-waste biomass have a high potential for developing thermostable cellulase required in the biofuel industry. The cost for cellulase represents a significant challenge in converting lignocellulose to fermentable sugars for biofuel production. Among three potential bacteria examined, Bacillus licheniformis 2D55 (accession no. KT799651) was found to produce the highest cellulolytic activity (CMCase 0.33 U/mL and FPase 0.09 U/mL) at 18-24 h fermentation when grown on microcrystalline cellulose (MCC) as a carbon source in shake flask at 50 °C. Cellulase production process was further conducted on the untreated and NaOH pretreated rice straw (RS), rice husk (RH), sugarcane bagasse (BAG) and empty fruit bunch (EFB). Untreated BAG produced the highest FPase (0.160 U/mL), while the highest CMCase (0.150 U/mL) was supported on the pretreated RH. The mixture of untreated BAG and pretreated RH as agro-waste cocktail has remarkably improved CMCase (3.7- and 1.4-fold) and FPase (2.5- and 11.5-fold) compared to the untreated BAG and pretreated RH, respectively. The mechanism of cellulase production explored through SEM analysis and the location of cellulase enzymes of the isolate was also presented. Agro-waste cocktail supplementation provides an alternative method for an efficient production of cellulase.

  9. Co-cultivation of Aspergillus nidulans Recombinant Strains Produces an Enzymatic Cocktail as Alternative to Alkaline Sugarcane Bagasse Pretreatment.

    Science.gov (United States)

    Lima, Matheus S; Damasio, André R de L; Crnkovic, Paula M; Pinto, Marcelo R; da Silva, Ana M; da Silva, Jean C R; Segato, Fernando; de Lucas, Rosymar C; Jorge, João A; Polizeli, Maria de L T de M

    2016-01-01

    Plant materials represent a strategic energy source because they can give rise to sustainable biofuels through the fermentation of their carbohydrates. A clear example of a plant-derived biofuel resource is the sugar cane bagasse exhibiting 60-80% of fermentable sugars in its composition. However, the current methods of plant bioconversion employ severe and harmful chemical/physical pretreatments raising biofuel cost production and environmental degradation. Replacing these methods with co-cultivated enzymatic cocktails is an alternative. Here we propose a pretreatment for sugarcane bagasse using a multi-enzymatic cocktail from the co-cultivation of four Aspergillus nidulans recombinant strains. The co-cultivation resulted in the simultaneous production of GH51 arabinofuranosidase (AbfA), GH11 endo-1,4-xylanase (XlnA), GH43 endo-1,5-arabinanase (AbnA) and GH12 xyloglucan specific endo-β-1,4-glucanase (XegA). This core set of recombinant enzymes was more efficient than the alternative alkaline method in maintaining the cellulose integrity and exposing this cellulose to the following saccharification process. Thermogravimetric and differential thermal analysis revealed residual byproducts on the alkali pretreated biomass, which were not found in the enzymatic pretreatment. Therefore, the enzymatic pretreatment was residue-free and seemed to be more efficient than the applied alkaline method, which makes it suitable for bioethanol production.

  10. Quantitative analysis of mitragynine, codeine, caffeine, chlorpheniramine and phenylephrine in a kratom (Mitragyna speciosa Korth.) cocktail using high-performance liquid chromatography.

    Science.gov (United States)

    Chittrakarn, Somsmorn; Penjamras, Pimpimol; Keawpradub, Niwat

    2012-04-10

    A simple HPLC technique for determining mitragynine, codeine, caffeine, chlorpheniramine and phenylephrine in 'kratom cocktail' was developed. The analytical method for mitragynine, codeine and caffeine used an Eclipse XDB-C8 column. A Lichrospher CN column was using for analysing chlorpheniramine and phenylephrine. The correlation coefficient of each standard was between 0.9957 and 0.9993. The precision of the methods were between 0.700 and 7.108% RSD. The concentration of mitragynine, codeine, caffeine, chlorpheniramine and phenylephrine in 'kratom cocktail' was 90.021, 234.174, 73.986, 7.053 and 1.486 mg/L, respectively.

  11. Screening for anabolic steroids and related compounds in illegal cocktails by liquid chromatography/time-of-flight mass spectrometry and liquid chromatography/quadrupole time-of-flight tandem mass spectrometry with accurate mass measurement

    NARCIS (Netherlands)

    Nielen, M.W.F.; Vissers, J.P.C.; Fuchs, R.E.M.; Velde, van J.W.; Lommen, A.

    2001-01-01

    Findings of illegal hormone preparations such as syringes, bottles, cocktails, and so on, are an important information source for the nature of the current abuse of anabolic steroids and related compounds as growth-promoting agents in cattle. A new screening method for steroids in cocktails is prese

  12. Quantum Clock Synchronization a Multi-Party Protocol

    CERN Document Server

    Krco, M; Krco, Marko; Paul, Prabasaj

    2001-01-01

    We present a multi-party quantum clock synchronization protocol that utilizes shared prior entanglement and broadcast of classical information to synchronize spatially separated clocks. Notably, it is necessary only for any one party to publish classical information. Consequently, the efficacy of the method is independent of the relative location of the parties. The suggested protocol is robust and does not require precise sequencing of procedural steps.

  13. Misconduct in third-party assisted reproduction: a committee opinion.

    Science.gov (United States)

    2014-01-01

    Physicians who are told or discover information that would be material to another party's participation in an assisted reproductive technology (ART) arrangement (such as lawyer's, donor's, gestational carrier's, or intended parent's) should encourage disclosure to that party. In some instances, it is ethically and legally permissive for the physician to either disclose material information to an affected party or to transfer care of a patient to another willing provider. In all cases involving the legal status or rights of the parties, referral to legal professionals is advised.

  14. Server-Aided Two-Party Computation with Simultaneous Corruption

    DEFF Research Database (Denmark)

    Cascudo Pueyo, Ignacio; Damgård, Ivan Bjerre; Ranellucci, Samuel

    We consider secure two-party computation in the client-server model where there are two adversaries that operate separately but simultaneously, each of them corrupting one of the parties and a restricted subset of servers that they interact with. We model security via the local universal composab......We consider secure two-party computation in the client-server model where there are two adversaries that operate separately but simultaneously, each of them corrupting one of the parties and a restricted subset of servers that they interact with. We model security via the local universal...

  15. SITE COMPREHENSIVE LISTING (CERCLIS) (Superfund) - Responsible Parties at CERCLIS Sites

    Data.gov (United States)

    U.S. Environmental Protection Agency — Responsible Parties at CERCLIS Sites - The Comprehensive Environmental Response, Compensation and Liability Information System (CERCLIS) (Superfund) Public Access...

  16. Organization, territory and party system: territorial diffusion of party organization and its potential impacts on the structure of the party system in Brazil

    Directory of Open Access Journals (Sweden)

    María do Socorro SOUSA BRAGA

    2013-01-01

    Full Text Available What is the territorial pattern of Brazilian political parties? What kind of relation this spatiality present with the party system? In order to answer these questions, this articles focus on three main objectives. Firstly, it will investigate the systematic pattern of political organization and affiliation in Brazilian municipalities. Secondly, it will perform an exploratory analysis of the spatial distribution of the political parties’ organization in Brazil. Third, it will produce some tentative hypotheses concerning the causal relations between party organization, party system structure, and electoral performance. Teoretically, this work will be based on the work of Panebianco (2005 [1988], as well as it will employ extensively some more recent analyses such as those from Katz and Mair (1995 and Gunther and Diamond (2003. The methodology used will, most of all, perform an Exploratory Spatial Data Analysis (esda. The results reveal that there were a constant movement towards expansion of party affiliation in Brazilian territory and it is manifested through a structural difference between large (with national territorial coverage and small parties (mostly regional in terms of party organization.

  17. The social organism: congresses, parties, and committees.

    Science.gov (United States)

    Strassmann, Joan E; Queller, David C

    2010-03-01

    We propose that what makes an organism is nearly complete cooperation, with strong control of intraorganism conflicts, and no affiliations above the level of the organism as unified as those at the organism level. Organisms can be made up of like units, which we call fraternal organisms, or different units, making them egalitarian organisms. Previous definitions have concentrated on the factors that favor high cooperation and low conflict, or on the adapted outcomes of organismality. Our approach brings these definitions together, conceptually unifying our understanding of organismality. Although the organism is a concerted cluster of adaptations, nearly all directed toward the same end, some conflict may remain. To understand such conflict, we extend Leigh's metaphor of the parliament of genes to include parties with different interests and committees that work on particular tasks.

  18. Social host policies and underage drinking parties.

    Science.gov (United States)

    Wagoner, Kimberly G; Sparks, Michael; Francisco, Vincent T; Wyrick, David; Nichols, Tracy; Wolfson, Mark

    2013-01-01

    Social host policies focused on underage drinking parties are implemented to reduce social availability of alcohol and high-risk drinking by adolescents in private locations. We examined the policies' relationship with drinking location, peer-group drinking size, heavy episodic drinking, and nonviolent consequences. Cross-sectional data from 11,205, 14-20-year olds, were analyzed using multilevel modeling. Policies were not associated with drinking location, decreased heavy episodic drinking, or nonviolent consequences. However, adolescents from communities with a preexisting policy had lower odds of drinking in large peer groups compared to those from communities without a policy at baseline. Additional research is needed to examine their effectiveness. The study's limitations are noted.

  19. ‘To quarterback behind the scenes, third-party efforts’: the tobacco industry and the Tea Party

    Science.gov (United States)

    Fallin, Amanda; Grana, Rachel; Glantz, Stanton A

    2014-01-01

    Background The Tea Party, which gained prominence in the USA in 2009, advocates limited government and low taxes. Tea Party organisations, particularly Americans for Prosperity and FreedomWorks, oppose smoke-free laws and tobacco taxes. Methods We used the Legacy Tobacco Documents Library, the Wayback Machine, Google, LexisNexis, the Center for Media and Democracy and the Center for Responsive Politics (opensecrets.org) to examine the tobacco companies’ connections to the Tea Party. Results Starting in the 1980s, tobacco companies worked to create the appearance of broad opposition to tobacco control policies by attempting to create a grassroots smokers’ rights movement. Simultaneously, they funded and worked through third-party groups, such as Citizens for a Sound Economy, the predecessor of AFP and FreedomWorks, to accomplish their economic and political agenda. There has been continuity of some key players, strategies and messages from these groups to Tea Party organisations. As of 2012, the Tea Party was beginning to spread internationally. Conclusions Rather than being a purely grassroots movement that spontaneously developed in 2009, the Tea Party has developed over time, in part through decades of work by the tobacco industry and other corporate interests. It is important for tobacco control advocates in the USA and internationally, to anticipate and counter Tea Party opposition to tobacco control policies and ensure that policymakers, the media and the public understand the longstanding connection between the tobacco industry, the Tea Party and its associated organisations. PMID:23396417

  20. Party-state relationship, an effect of the political competition. The party system and patronage in Romanian politics

    Directory of Open Access Journals (Sweden)

    Irina Lonean

    2012-06-01

    Full Text Available The article examines the party-state relationship in post-communist Romania. It shows the connections between the existence of patronage, corruption and the states’ weak administrative capacity, on one hand, and the dynamics of the political party system, as an explanatory variable, on the other hand. The instability of the political parties in Romania and their changing relations within the system make the electorates’ task of anticipating and sanctioning their politics impossible. Consequently, the political parties have the possibility of extracting resources from the state without being held accountable in elections, as an effect of their dynamic, but non-robust competition.

  1. Improving simultaneously the quality and safety of cooked and peeled shrimp using a cocktail of bioprotective lactic acid bacteria.

    Science.gov (United States)

    Saraoui, Taous; Cornet, Josiane; Guillouet, Emilie; Pilet, Marie France; Chevalier, Frédérique; Joffraud, Jean-Jacques; Leroi, Françoise

    2017-01-16

    Tropical shrimp is of considerable economic importance in the world but is highly perishable due to microbial and chemical degradation. Biopreservation is a food preservation technology based on the addition of "positive" bacteria able to kill or prevent the growth of undesirable microorganisms. Two strains of lactic acid bacteria (LAB) have previously been selected for a biopreservation strategy: Lactococcus piscium CNCM I-4031, for its ability to prevent the sensory deterioration of seafood and Carnobacterium divergens V41, which inhibits growth of Listeria monocytogenes. The objective was to test the association of the two strains to improve both the quality and safety of shrimp. In a first trial, the two LAB were inoculated alone or in a cocktail in cooked and peeled shrimp (CPS) Penaeus vannamei at 5×10(5)CFU/g. Chemical, sensory and microbiological analyses by culture-dependent and -independent methods were performed during storage under modified atmosphere packaging (MAP) at 8°C. The results were compared to a non-inoculated batch. In a second trial, the same experiments were repeated in the presence of 10(2)CFU/g of L. monocytogenes RF191. The microbiota of CPS was composed of LAB, Shewanella spp. and Enterobacteriaceae. Brochothrix thermosphacta was not detected. L. piscium and C. divergens reached 10(8) and 10(9)CFU/g, respectively, in 7days and did not inhibit each other when co-inoculated. L. piscium reduced L. monocytogenes by 1Log (CFU/g) for 28days. C. divergens had an immediate listericidal effect lasting 7days. A regrowth of L. monocytogenes was then observed but the count was always 2 to 5Log (CFU/g) lower than in the control. No additional or synergic effect between protective strains was observed and the cocktail had the same inhibitory effect as C. divergens alone. C. divergens was very effective at preventing the sensory deterioration of CPS. This may be related to the inhibition of Shewanella and Enterobacteriaceae. However, the panelists

  2. Political cleavages in Romania. A theoretical overview of the post-communist parties and party systems

    OpenAIRE

    Olimid, Anca Parmena

    2009-01-01

    The following article examines the emergence of parties in post-communist Romania concluding that the theoretical basis of cleavages (the Lipset-Rokkanian model) is almost impossible to apply in this country. Accordingly, the historical evolution of the post-communist society and the strategic moves of political actors during democratization were often perceived as an „expected moments lacking any theoretical model”. Contrary to this assumption, I argue that even an incipient cleavage suggest...

  3. A novel analytical method for D-glucosamine quantification and its application in the analysis of chitosan degradation by a minimal enzyme cocktail

    DEFF Research Database (Denmark)

    Mekasha, Sophanit; Toupalová, Hana; Linggadjaja, Eka

    2016-01-01

    action of endo- and exo- chitosanases. In the present study we have developed an efficient and cost-effective chitosan-degrading enzyme cocktail containing only two enzymes, an endo-attacking bacterial chitosanase, ScCsn46A, from Streptomyces coelicolor, and an exo-attacking glucosamine specific β...

  4. Studies on cocktails of 31-kDa, 36-kDa and 51-kDa antigens of Leishmania donovani along with saponin against murine visceral leishmaniasis.

    Science.gov (United States)

    Kaur, H; Thakur, A; Kaur, S

    2015-04-01

    A substantial number of antigens of Leishmania donovani have been described in the past. However, identifying candidate antigens is not enough. Appropriate antigen delivery to induce the right type of immune response against leishmaniasis (i.e. induction of a strong antigen-specific Th1 type of immune response) is another crucial component of an effective vaccine. Therefore, 'cocktail' vaccines are proposed based on the assumption that such cocktails will show enhanced efficacy. Studies have been carried out on LD31 and LD51 polypeptides from L. donovani promastigotes, which have proven to be potential vaccine candidates. This study was designed to check the protective efficacy of various cocktails of low molecular weight antigens alone and along with saponin as adjuvant. Mice were sacrificed on different post-challenge days for evaluation of parasite load and other immunological parameters. Protective efficacy of different vaccine formulations was revealed by significant decline in parasite burden and increased DTH Delayed Type Hypersenstivity responses. The antibody response was of IgG type with elevated IgG2a and decreased production of IgG1, whereas cytokine levels pointed towards the generation of protective Th1 type of immune response. Among all vaccine formulations, cocktail of 31+51+saponin was found to be highly immunogenic and imparted maximum protection.

  5. An in vitro bioassay for the quantitative evaluation of mosquito repellents against Stegomyia aegypti (=Aedes aegypti) mosquitoes using a novel cocktail meal.

    Science.gov (United States)

    Huang, T-H; Tien, N-Y; Luo, Y-P

    2015-09-01

    To assess the efficacy of new insect repellents, an efficient and safe in vitro bioassay system using a multiple-membrane blood-feeding device and a cocktail meal was developed. The multiple-membrane blood-feeding device facilitates the identification of new insect repellents by the high-throughput screening of candidate chemicals. A cocktail meal was developed as a replacement for blood for feeding females of Stegomyia aegypti (=Aedes aegypti) (L.) (Diptera: Culicidae). The cocktail meal consisted of a mixture of salt, albumin and dextrose, to which adenosine triphosphate was added to induce engorging. Feeding rates of St. aegypti on the cocktail meal and pig blood, respectively, did not differ significantly, but were significantly higher than the feeding rate on citrate phosphate dextrose-adenine 1 (CPDA-1) solutions, which had been used to replace bloodmeals in previous repellent assays. Dose-dependent biting inhibition rates were analysed using probit analysis. The RD(50) (the dose producing 50% repellence of mosquito feeding) values of DEET, citronella, carvacrol, geraniol, eugenol and thymol were 1.62, 14.40, 22.51, 23.29, 23.83 and 68.05 µg/cm(2), respectively.

  6. Political parties and Facebook: A study of Icelandic political parties and their social media usage

    Directory of Open Access Journals (Sweden)

    Baldvin Þór Bergsson

    2014-12-01

    Full Text Available The importance of social media in the national discourse is increasing but little is known about their true effects on political communication and participation. The paper examines how the Icelandic political parties used social media during the campaign for the 2013 general elections and possible influence of the electorate. Data from the Icelandic National Election Study was used to examine a possible link between political interest and participation. International studies are used as a reference since Icelandic studies on the subject are limited, and a theoretical overview of the nature and effect of social media is provided. The findings of this paper are that social media was primarily used as a one-way communication tool and that interaction between parties and the electorate was limited. Facebook is by far the most important social media due to its spread and easiness to send information to a large group. The study does not find evidence for the claim that voters had much effect on the campaign through social media nor that social media affected the election results. People with much interest in politics are more likely to use the internet to receive information from the parties.

  7. 12 CFR 269a.2 - Party in interest.

    Science.gov (United States)

    2010-01-01

    ... employees of a bank or that is or was recently a party to a collective bargaining agreement with a bank... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Party in interest. 269a.2 Section 269a.2 Banks... employees, labor organization, or bank that will be or is directly affected by the resolution of any charge...

  8. Supreme matters: tea parties and the activism of restraint

    NARCIS (Netherlands)

    Kurzbauer, H.

    2010-01-01

    An essay on the impact of Tea Parties on the appointment of Supreme Court Justices. According to recently released statistics, one-fifth of all US citizens are Tea Party Supporters: predominately white conservatives convinced of the 'evils' of the Obama administration. Taking their cue from the

  9. 29 CFR 552.109 - Third party employment.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Third party employment. 552.109 Section 552.109 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR REGULATIONS APPLICATION OF THE FAIR LABOR STANDARDS ACT TO DOMESTIC SERVICE Interpretations § 552.109 Third party employment...

  10. Developing Product Lines with 3rd-party components

    NARCIS (Netherlands)

    De Jonge, M.

    2007-01-01

    The trends toward product line development and toward adopting more 3rd-party software are hard to combine. The reason is that productlines demand fine control over the software (e.g., for diversity management), while 3rd-party software (almost by definition) provides only little or no control. A

  11. Features of modern security policy UK political parties

    Directory of Open Access Journals (Sweden)

    A. V. Stalovierova

    2015-12-01

    Full Text Available The security policy of the British coalition government of D. Cameron (2010-2015 is summarized; it’s been proved that political forces of Tory and Liberal Democrat should have compromised to carry out unanimous course in the scope of national security, and the problems of the security strategy on parliamentary elections in 2015 are analyzed, particularly the comparative analysis of the modern safety strategy of leading British parties is exercised. Under conditions of the appearance of new challenges and threats, transformation of international safety system, the questions of safety policy often become the object of attention of the British community and experts. The absence of cross-party consensus on most terms of safety strategy of the United Kingdom during the election campaign in 2015 makes the discussion about perspectives of the British safety policy still more urgent. During the election campaign there was no unity on any aspect of security subject between parties. First of all, Labourists, Liberal Democrats, Scottish National Party and Green Party made a statement about readiness to develop cooperation with the EU and the Conservatives and the UK Independence Party were on the side of the Eurosceptics. The opinions of the parties were also divided on military operations abroad, financing and force level. In terms of one-party government and presence of majority in the House of Commons, the Conservatives have opportunities to realize their own vision of British safety policy.

  12. The Effects of Third-Party Arbitration: A Field Experiment

    NARCIS (Netherlands)

    Joosten, H.W.M.; Bloemer, J.M.M.; Hillebrand, B.

    2016-01-01

    Governments, firms, and consumer agencies promote third-party arbitration to end consumer–firm disputes that arise from dissatisfying services and failed service recoveries in the hope that third-party arbitration will (1) resolve the dispute, (2) repair the relationship with the service provider, a

  13. The Impact of Having Children on Party Member Activism

    DEFF Research Database (Denmark)

    Kosiara-Pedersen, Karina

    2014-01-01

    Party member activism varies in type and intensity, and it is necessary to take this into consideration when explaining the degree of participation. One potential explanation for the level of party member participation is the extent to which there are children in the household. Based on the Danis...

  14. 17 CFR 201.150 - Service of papers by parties.

    Science.gov (United States)

    2010-04-01

    ... 17 Commodity and Securities Exchanges 2 2010-04-01 2010-04-01 false Service of papers by parties... PRACTICE Rules of Practice General Rules § 201.150 Service of papers by parties. (a) When required. In every proceeding as defined in § 201.101(a), each paper, including each notice of appearance,...

  15. 12 CFR 308.516 - Rights of parties.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Rights of parties. 308.516 Section 308.516 Banks and Banking FEDERAL DEPOSIT INSURANCE CORPORATION PROCEDURE AND RULES OF PRACTICE RULES OF PRACTICE AND PROCEDURE Program Fraud Civil Remedies and Procedures § 308.516 Rights of parties. Except as...

  16. 12 CFR 308.512 - Parties to the hearing.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Parties to the hearing. 308.512 Section 308.512 Banks and Banking FEDERAL DEPOSIT INSURANCE CORPORATION PROCEDURE AND RULES OF PRACTICE RULES OF PRACTICE AND PROCEDURE Program Fraud Civil Remedies and Procedures § 308.512 Parties to the hearing. (a...

  17. Complete Fairness in Secure Two-Party Computation

    DEFF Research Database (Denmark)

    Gordon, S. Dov; Hazay, Carmit; Katz, Jonathan

    2011-01-01

    In the setting of secure two-party computation, two mutually distrusting parties wish to compute some function of their inputs while preserving, to the extent possible, various security properties such as privacy, correctness, and more. One desirable property is fairness which guarantees, informa......In the setting of secure two-party computation, two mutually distrusting parties wish to compute some function of their inputs while preserving, to the extent possible, various security properties such as privacy, correctness, and more. One desirable property is fairness which guarantees......, informally, that if one party receives its output, then the other party does too. Cleve [1986] showed that complete fairness cannot be achieved in general without an honest majority. Since then, the accepted folklore has been that nothing non-trivial can be computed with complete fairness in the two......-party setting. We demonstrate that this folklore belief is false by showing completely fair protocols for various nontrivial functions in the two-party setting based on standard cryptographic assumptions. We first show feasibility of obtaining complete fairness when computing any function over polynomial...

  18. When the stakes are high: party competition and negative campaigning

    NARCIS (Netherlands)

    A.S. Walter; W. van der Brug; P. van Praag

    2013-01-01

    This article examines the conditions under which different kinds of parties resort to negative campaigning in three Western European countries: the Netherlands, Britain, and Germany in the period between 1980 and 2006. Data were collected for 27 parties, participating in 23 elections, yielding a tot

  19. STEM and the Evolution of the Astronomical Star Party

    Science.gov (United States)

    Day, B. H.; Munive, P.; Franco, J.; Jones, A. P.; Shaner, A. J.; Buxner, S.; Bleacher, L.

    2015-12-01

    The astronomical star party has long been a powerful and effective way to engage the public and enhance cohesiveness within the amateur astronomy community. Early star parties tended to be strictly small, local events. But with improvements in transportation, larger regional star parties became popular. These advanced the considerable capabilities for citizen science in the amateur community, shared technology and engineering innovations in the field of telescope making, and refined numerous mathematical techniques in areas such instrument design and ephemeris generation, covering the full breadth of STEM. Advancements in astrophotography showcased at these events brought the star party from STEM to STEAM. Now, the advent of social media, web streaming, and virtual presence has facilitated the phenomenon of very large, networked star parties with international scope. These mega star parties take public engagement to a new, far greater levels, giving a vastly larger and more diverse public the opportunity to directly participate in exciting first-hand STEM activities. This presentation will recount the evolution of the star party and will focus on two examples of large, multinational, networked star parties, International Observe the Moon Night and Noche de las Estrellas. We will look at lessons learned and ways to participate.

  20. Anteneh Geremew Gemeda Abstract Ethiopia is party to the ...

    African Journals Online (AJOL)

    Ethiopia is party to the Convention on the Rights of the Child and its two ... The Committee on the Right of the Child is the organ entrusted with monitoring states' .... rights observance are essential behavior of the Parties to consideration of the report. ... Santos who believes that “concluding observations are authoritative ...

  1. Multi-party arbitration in international trade: problems and solutions

    DEFF Research Database (Denmark)

    Siig, Kristina

    2007-01-01

    of dispute resolution in a multy-party set-up. Both the possible legal bases and the problems encountered are considere. It is concluded that arbitration is still the only real option to the parties in international business disputes and that many of the shortcomings  may be contered by skilful drafting...

  2. 16 CFR 240.13 - Customer's and third party liability.

    Science.gov (United States)

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Customer's and third party liability. 240.13 Section 240.13 Commercial Practices FEDERAL TRADE COMMISSION GUIDES AND TRADE PRACTICE RULES GUIDES FOR ADVERTISING ALLOWANCES AND OTHER MERCHANDISING PAYMENTS AND SERVICES § 240.13 Customer's and third party...

  3. A Critical Discourse Analysis of the 315 Evening Party

    Institute of Scientific and Technical Information of China (English)

    胡喜喜

    2014-01-01

    According to Norman Fairclough’s three-dimensional model of discourse analysis, this paper studies the transcription of the 315 evening party to explore the ideologies hidden behind. Based on the analysis, this paper concludes that the coexistence of the legal conscience and the ethnics and their competing relationship are the underlying causes of the emergence of the 315 evening party.

  4. Organizational mediators siding with or against the powerful party

    NARCIS (Netherlands)

    Laskewitz, P; Van de Vliert, E; De Dreu, C K W

    1994-01-01

    Instead of being impartial, a mediator may side with one party as a function of the disputants' power differences, the mediator's legitimacy judgments, or the disputants' capacity to sanction the mediator. According to the power balancing theory, a mediator sides with the party that has a power disa

  5. Supreme matters: tea parties and the activism of restraint

    NARCIS (Netherlands)

    Kurzbauer, H.

    2010-01-01

    An essay on the impact of Tea Parties on the appointment of Supreme Court Justices. According to recently released statistics, one-fifth of all US citizens are Tea Party Supporters: predominately white conservatives convinced of the 'evils' of the Obama administration. Taking their cue from the defi

  6. 29 CFR 18.18 - Written interrogatories to parties.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 1 2010-07-01 2010-07-01 true Written interrogatories to parties. 18.18 Section 18.18 Labor Office of the Secretary of Labor RULES OF PRACTICE AND PROCEDURE FOR ADMINISTRATIVE HEARINGS BEFORE THE OFFICE OF ADMINISTRATIVE LAW JUDGES General § 18.18 Written interrogatories to parties....

  7. INDUCTION OF ENZYME COCKTAILS BY LOW COST CARBON SOURCES FOR PRODUCTION OF MONOSACCHARIDE-RICH SYRUPS FROM PLANT MATERIALS

    Directory of Open Access Journals (Sweden)

    Caroline T. Gilleran

    2010-05-01

    Full Text Available The production of cellulases, hemicellulases, and starch-degrading enzymes by the thermophilic aerobic fungus Talaromyces emersonii under liquid state culture on various food wastes was investigated. A comprehensive enzyme screening was conducted, which resulted in the identification of spent tea leaves as a potential substrate for hydrolytic enzyme production. The potent, polysaccharide-degrading enzyme-rich cocktail produced when tea leaves were utilised as sole carbon source was analysed at a protein and mRNA level and shown to exhibit high level production of key cellulose and hemicellulose degrading enzymes. As presented in this paper, the crude enzyme preparation produced after 120 h growth of Talaromyces emersonii on used tea leaves is capable of hydrolysing other lignocellulosic materials into their component monosaccharides, generating high value sugar syrups with a host of industrial applications including conversion to fuels and chemicals.

  8. Pre-selecting resistance against individual Bti Cry toxins facilitates the development of resistance to the Bti toxins cocktail.

    Science.gov (United States)

    Stalinski, Renaud; Tetreau, Guillaume; Gaude, Thierry; Després, Laurence

    2014-06-01

    The bioinsecticide Bacillus thuringiensis subsp. israelensis is a larvicide used worldwide for mosquito control, which contains three Cry toxins and one Cyt toxin. We investigated for the first time in Aedes aegypti (1) the evolution of resistance and cross-resistance of strains selected with each Cry toxin, and (2) the effect of pre-selection with Cry toxin on the evolution of resistance to a mix of Bti toxins. Cross resistance was higher between Cry4Ba and Cry11Aa than between Cry4Aa and either Cry4Ba or Cry11Aa, suggesting both common and specific mechanisms of resistance. Pre-selecting resistance to each Cry toxins facilitated the development of resistance to the full Bti toxins cocktail. Copyright © 2014 Elsevier Inc. All rights reserved.

  9. The armed hand of State-party: The National Guard

    Directory of Open Access Journals (Sweden)

    Florin Grecu

    2014-11-01

    Full Text Available The single party was the militarized political institution of the new authoritarian regime lead by King Carol II by using the armed hand of single organization in the state, The National Guard. The militarization of policy represented the main dimension of the regime and the state architecture revealed the single party capacity to militarize the administrative institutions. The architecture of National Renaissance Front was similar to a military unit, the official attributions of the National Guard consisted in the maintenance of order in the territory, and unofficially regarded the monitoring of members and opposites of the party and regime, by control and surveillance activity. The National Guard was politicized institution of the regime and of the single party which proved its inefficiency because of the subordination of the commandant general to the single party. Militaries lead the single organization as well as the National Guard.

  10. Impact of a pesticide cocktail (fenhexamid, folpel, deltamethrin) on the abundance of Glomeromycota in two agricultural soils.

    Science.gov (United States)

    Rivera-Becerril, Facundo; van Tuinen, Diederik; Chatagnier, Odile; Rouard, Nadine; Béguet, Jérémie; Kuszala, Catherine; Soulas, Guy; Gianinazzi-Pearson, Vivienne; Martin-Laurent, Fabrice

    2017-01-15

    Pesticide contamination of the environment can result from agricultural practices. Persistence of pesticide residues is a threat to the soil biota including plant roots and beneficial microorganisms, which support an important number of soil ecosystem services. Arbuscular mycorrhizal fungi (AMF) are key symbiotic microorganisms contributing to plant nutrition. In the present study, we assessed whether AMF could indicate eventual side effects of pesticides when directly applied to field soils. We evaluated the ecotoxicological impact of a cocktail of three commonly used agricultural pesticides (fenhexamid, folpel, deltamethrin) on the abundance and composition of the AMF community in vineyard (Montagne de Saint-Emilion) and arable (Martincourt) soils subjected to different agricultural practices. The dissipation of applied pesticides was monitored by multiresidual analyses to determine the scenario of exposure of the AMF community. Diversity analysis before application of the pesticide cocktail showed that the AMF communities of vineyard soils, subjected to mechanical weeding or grass cover, and of the arable soil subjected to intensive agriculture, were dominated by Glomerales. Ribotypes specific to each soil and to each agricultural practice in the same soil were found, with the highest abundance and diversity of AMF being observed in the vineyard soil with a grass-cover. The abundance of the global AMF community (Glomeromycota) and of three taxa of AMF (Funneliformis mosseae, Claroideoglomus etunicatum/C. claroideum) was evaluated after pesticide application. The abundance of Glomeromycota decreased in both soils after pesticide application while the abundance of Claroideoglomus and F. mosseae decreased only in the arable soil. These results show that higher doses of pesticide exposure did not affect the global abundance, but altered the composition, of the AMF community. Resilience of the AMF community composition was observed only in the vineyard soil, where F

  11. Effect of a bacteriophage cocktail in combination with modified atmosphere packaging in controlling Listeria monocytogenes on fresh-cut spinach

    Directory of Open Access Journals (Sweden)

    Boyacioglu O.

    2016-06-01

    Full Text Available A Listeria monocytogenes-specific bacteriophage cocktail was evaluated for its activity against a nalidixic acid-resistant L. monocytogenes (Lm-NalR isolate on fresh-cut spinach stored under modified atmosphere packaging at various temperatures. Pieces (~2 × 2 cm2 of fresh spinach inoculated with 4.5 log CFU/cm2 Lm-NalR were sprayed with the phage cocktail (6.5 log plaque-forming units [PFU]/cm2 or a control. The samples were stored at 4°C or 10°C for up to 14 d in sealed packages filled with either atmospheric air (AA or modified atmosphere (MA. At 4°C under AA, the phages significantly (P ≤ 0.05 lowered the Lm-NalR populations on spinach, compared to control-treated inoculated samples, by 1.12 and 1.51 log CFU/cm2 after 1 and 14 d, respectively. At 4°C under MA, Lm-NalR was significantly reduced by 1.95 log CFU/cm2 compared to control leaves after both 1 and 14 d. At 10°C under AA, the phages significantly reduced Lm-NalR by 1.50 and 2.51 log CFU/cm2 after 1 and 14 d compared to the control. Again at 10°C under MA, the phages significantly reduced Lm-NalR by 1.71 and 3.24 log CFU/cm2 compared to control after 1 and 14 d, respectively. The results support the potential of lytic bacteriophages in effectively reducing populations of L. monocytogenes on freshcut leafy produce, under both AA and MA conditions.

  12. Application of a cocktail approach to screen cytochrome P450 BM3 libraries for metabolic activity and diversity.

    Science.gov (United States)

    Reinen, Jelle; Postma, Geert; Tump, Cornelis; Bloemberg, Tom; Engel, Jasper; Vermeulen, Nico P E; Commandeur, Jan N M; Honing, Maarten

    2016-02-01

    In the present study, the validity of using a cocktail screening method in combination with a chemometrical data mining approach to evaluate metabolic activity and diversity of drug-metabolizing bacterial Cytochrome P450 (CYP) BM3 mutants was investigated. In addition, the concept of utilizing an in-house-developed library of CYP BM3 mutants as a unique biocatalytic synthetic tool to support medicinal chemistry was evaluated. Metabolic efficiency of the mutant library towards a selection of CYP model substrates, being amitriptyline (AMI), buspirone (BUS), coumarine (COU), dextromethorphan (DEX), diclofenac (DIC) and norethisterone (NET), was investigated. First, metabolic activity of a selection of CYP BM3 mutants was screened against AMI and BUS. Subsequently, for a single CYP BM3 mutant, the effect of co-administration of multiple drugs on the metabolic activity and diversity towards AMI and BUS was investigated. Finally, a cocktail of AMI, BUS, COU, DEX, DIC and NET was screened against the whole in-house CYP BM3 library. Different validated quantitative and qualitative (U)HPLC-MS/MS-based analytical methods were applied to screen for substrate depletion and targeted product formation, followed by a more in-depth screen for metabolic diversity. A chemometrical approach was used to mine all data to search for unique metabolic properties of the mutants and allow classification of the mutants. The latter would open the possibility of obtaining a more in-depth mechanistic understanding of the metabolites. The presented method is the first MS-based method to screen CYP BM3 mutant libraries for diversity in combination with a chemometrical approach to interpret results and visualize differences between the tested mutants.

  13. Harvesting (67)Cu from the Collection of a Secondary Beam Cocktail at the National Superconducting Cyclotron Laboratory.

    Science.gov (United States)

    Mastren, Tara; Pen, Aranh; Loveless, Shaun; Marquez, Bernadette V; Bollinger, Elizabeth; Marois, Boone; Hubley, Nicholas; Brown, Kyle; Morrissey, David J; Peaslee, Graham F; Lapi, Suzanne E

    2015-10-20

    Isotope harvesting is a promising new method to obtain isotopes for which there is no reliable continuous supply at present. To determine the possibility of obtaining radiochemically pure radioisotopes from an aqueous beam dump at a heavy-ion fragmentation facility, preliminary experiments were performed to chemically extract a copper isotope from a large mixture of projectile fragmentation products in an aqueous medium. In this work a 93 MeV/u secondary beam cocktail was collected in an aqueous beam stop at the National Superconducting Cyclotron Laboratory (NSCL) located on the Michigan State University (MSU) campus. The beam cocktail consisted of ∼2.9% (67)Cu in a large mixture of co-produced isotopes ranging in atomic number from ∼19 to 34. The chemical extraction of (67)Cu was achieved via a two-step process: primary extraction using a divalent metal chelation disk followed by anion-exchange chromatography. A significant fraction (74 ± 4%) of the (67)Cu collected in the aqueous beam stop was recovered with >99% radiochemical purity. To illustrate the utility of this product, the purified (67)Cu material was then used to radiolabel an anti-EGFR antibody, Panitumumab, and injected into mice bearing colon cancer xenografts. The tumor uptake at 5 days postinjection was found to be 12.5 ± 0.7% which was in very good agreement with previously reported studies with this radiolabeled antibody. The present results demonstrate that harvesting isotopes from a heavy-ion fragmentation facility could be a promising new method for obtaining high-quality isotopes that are not currently available by traditional methods.

  14. Impact of a Single Phage and a Phage Cocktail Application in Broilers on Reduction of Campylobacter jejuni and Development of Resistance

    Science.gov (United States)

    Fischer, Samuel; Kittler, Sophie; Klein, Günter; Glünder, Gerhard

    2013-01-01

    Campylobacteriosis is currently the most frequent foodborne zoonosis in many countries. One main source is poultry. The aim of this study was to enhance the knowledge about the potential of bacteriophages in reducing colonization of broilers with Campylobacter , as there are only a few in vivo studies published. Commercial broilers were inoculated with 104 CFU/bird of a Campylobacter jejuni field strain. Groups of 88 birds each were subsequently treated with a single phage or a four-phage cocktail (107 PFU/bird in CaCO3 buffered SM-Buffer). Control birds received the solvent only. Afterwards, subgroups of eleven birds each were examined for their loads with phages and Campylobacter on day 1, 3, 7, 14, 21, 28, 35 and 42 after phage application. The susceptibility of the Campylobacter population to phage infection was determined using ten isolates per bird. In total 4180 re-isolates were examined. The study demonstrated that the deployed phages persisted over the whole investigation period. The Campylobacter load was permanently reduced by the phage-cocktail as well as by the single phage. The reduction was significant between one and four weeks after treatment and reached a maximum of log10 2.8 CFU/g cecal contents. Phage resistance rates of initially up to 43% in the single phage treated group and 24% in the cocktail treated group later stabilized at low levels. The occurrence of phage resistance influenced but did not override the Campylobacter reducing effect. Regarding the reduction potential, the cocktail treatment had only a small advantage over the singe phage treatment directly after phage administration. However, the cocktail moderated and delayed the emergence of phage resistance. PMID:24205254

  15. Matrix revolutions? An analysis of party organization and ICT use by political parties in the Republic of Ireland

    NARCIS (Netherlands)

    Wall, M.; Sudulich, M.L.

    2010-01-01

    This article investigates the characteristics of political parties' websites in the Republic of Ireland and seeks to evaluate whether parties' organizational structures influence the manner in which they present themselves online. Ireland has been chosen as a research environment because there is si

  16. Parties beyond the pale: why some political parties are ostracized by their competitors while others are not

    NARCIS (Netherlands)

    van Spanje, J.

    2010-01-01

    Since the 1960s, anti-immigration parties have emerged in many established European democracies. Some of them - for example, the German Republikaner and the Vlaams Belang in Belgium - have been treated as pariahs by other parties. Others - for example, the Lega Nord in Italy and the Dutch Partij voo

  17. Contagious parties: anti-immigration parties and their impact on other parties’ immigration stances in contemporary Western Europe

    NARCIS (Netherlands)

    van Spanje, J.

    2010-01-01

    Anti-immigration parties have experienced electoral lift-off in most Western democracies, although the consequences of their victories for real-life policy outcomes have remained largely unexplored. A key question is: do electoral pressures from anti-immigration parties have a ‘contagion’ impact on

  18. Matrix revolutions? An analysis of party organization and ICT use by political parties in the Republic of Ireland

    NARCIS (Netherlands)

    Wall, M.; Sudulich, M.L.

    2010-01-01

    This article investigates the characteristics of political parties' websites in the Republic of Ireland and seeks to evaluate whether parties' organizational structures influence the manner in which they present themselves online. Ireland has been chosen as a research environment because there is si

  19. Cops and dogs against party drugs

    Directory of Open Access Journals (Sweden)

    Ton Nabben

    2010-10-01

    Full Text Available

    In the Netherlands, a significant shift in policy with regard to drug users can be observed. Originally, ecstasy was predominantly defined as a ‘pleasure pill’, whereas today, Dutch policy makers increasingly associate the substance with health problems. The police are present at festivals and dance parties, bouncers are instructed to (body-search all visitors, and police dogs are used at the venue to detect drugs. Theoretically framed by a critical discussion of the historical and theoretical roots of zero-tolerance, this article uses qualitative and quantitative data to answer three questions, relating to the national level and more specifically to Amsterdam. What is the effect of this new zero tolerance policy in practice? How have drug users and drug dealers reacted to the repressive measures, which sometimes include drug detection dogs? What developments have occurred in the drug trade and in the use of recreational substances?

  20. Monday (after work) is party time!

    CERN Multimedia

    Antonella Del Rosso

    2013-01-01

    In this interview with Hermann Schmickler, CERN accelerator expert and, more recently, manager of the Open Day organising team, the Bulletin looks behind the scenes of the organisation of CERN’s most popular events for the public.  After the huge endeavour, the “Bosons&More” party awaits you too!   The Open Days core team. “In the early days of the preparations when we still had some spare time, we decided to take photos in two different dress codes to illustrate the spirit of “Bosons&More”: formal evening dress and festival type clothes,” says Herman Schmickler. What will your dress code be on Monday evening?   When Hermann is asked “what did you like best” about this whole adventure, he has no hesitation: “The team,” he says. “We have a wonderful team of very motivated people.” Let’s make a rough calculation: the core team compris...

  1. CERN clubs join the fiftieth anniversary party!

    CERN Multimedia

    2004-01-01

    CERN's clubs are planning to celebrate the Organization's 50th anniversary by organising a variety of special events. It's raining projects! CERN's clubs have chosen to celebrate the Organization's Golden Jubilee, each in its own style, by organising a whole host of anniversary events. While the Cine-Club continues to screen films made in the Member States on Thursday evenings, other clubs are organising special races and tournaments to honour CERN's 50th year. The CERN Rugby Club led the way in early April when it hosted a children's tournament which brought the Laboratory firmly into the view of its many young participants. On 24 April, the Cycling Club moves to centre stage as it takes its members on a unique 50-km discovery trip around the countryside above the tunnel of the future LHC. The Croquet Club, the Running Club, the Horse Riding Club and the Golf Club will all be joining the party in May and June. After that, football, softball, cycling, pétanque and sailing are just some of the items on a sp...

  2. Intra-party policy entrepreneurship and party goals. The case of political parties’ climate policy preferences in Ireland

    DEFF Research Database (Denmark)

    Little, Conor

    2017-01-01

    This study contributes to the growing literature on the domestic politics of climate change by examining the climate policy preferences of Fianna Fáil, Fine Gael and the Labour Party over 20 years. Bringing the concept of ‘policy entrepreneurship’ into the study of intra-party politics, it uses...... secondary data, party documents, publicly-available information and a series of in-depth interviews to develop an understanding of intra-party policy entrepreneurship and political parties’ climate policy preferences. It finds that office-seeking incentives have tended to trump the activities of individuals...... who have engaged in policy entrepreneurship with the aim of strengthening their parties’ climate policy preferences. In instances where office-seeking incentives for climate policy have been strong, they have often contributed to changes in policy preferences, whether or not intra-party policy...

  3. Immunological evaluation of a DNA cocktail vaccine with co-delivery of calcium phosphate nanoparticles (CaPNs) against the Toxoplasma gondii RH strain in BALB/c mice.

    Science.gov (United States)

    Rahimi, Mohammad Taghi; Sarvi, Shahabeddin; Sharif, Mahdi; Abediankenari, Saeid; Ahmadpour, Ehsan; Valadan, Reza; Ramandie, Mahdi Fasihi-; Hosseini, Seyed-Abdollah; Daryani, Ahmad

    2017-02-01

    Many recent studies have been conducted to evaluate protective immunity mediated by DNA vaccines against toxoplasmosis. Cocktail DNA vaccines showed better immune responses compared to single vaccines. The objective of the current study was to evaluate the protective efficacy of rhomboid 4 (ROM4) and cocktail DNA vaccines (ROM4 + GRA14) of the Toxoplasma gondii RH strain with or without coated calcium phosphate nanoparticles (CaPNs) as the adjuvant to improve the immunogenicity against the T. gondii RH strain in BALB/c mice. Cocktail DNA vaccines of pcROM4 + pcGRA14 of the T. gondii RH strain were constructed. CaPNs were synthesized and the cocktail DNA vaccine was coated with the adjuvant of CaPNs. Immunogenicity and the protective effects of cocktail DNA vaccines with or without CaPNs against lethal challenge were evaluated in BALB/c mice. pcROM4 and cocktail DNA vaccine coated with CaPNs significantly enhanced cellular and humoral immune responses against Toxoplasma compared to pcROM4 and cocktail DNA vaccine without CaPNs (p < 0.05). These findings indicate that the survival time of immunized mice after challenge with the RH strain of T. gondii was increased compared to that of controls and the DNA vaccine provided significant protection in mice (p < 0.05). The CaPN-based cocktail DNA vaccine of pcROM4 + pcGRA14 showed the longest survival time compared to the other groups. Co-immunization with CaPN-based cocktail DNA vaccine (pcROM4 + pcGRA14) boosted immune responses and increased the protective efficacy against acute toxoplasmosis in BALB/c mice compared to both single gene and bivalent DNA vaccine without nano-adjuvants.

  4. Race, ideology, and the tea party: a longitudinal study.

    Science.gov (United States)

    Knowles, Eric D; Lowery, Brian S; Shulman, Elizabeth P; Schaumberg, Rebecca L

    2013-01-01

    The Tea Party movement, which rose to prominence in the United States after the election of President Barack Obama, provides an ideal context in which to examine the roles of racial concerns and ideology in politics. A three-wave longitudinal study tracked changes in White Americans' self-identification with the Tea Party, racial concerns (prejudice and racial identification), and ideologies (libertarianism and social conservatism) over nine months. Latent Growth Modeling (LGM) was used to evaluate potential causal relationships between Tea Party identification and these factors. Across time points, racial prejudice was indirectly associated with movement identification through Whites' assertions of national decline. Although initial levels of White identity did not predict change in Tea Party identification, initial levels of Tea Party identification predicted increases in White identity over the study period. Across the three assessments, support for the Tea Party fell among libertarians, but rose among social conservatives. Results are discussed in terms of legitimation theories of prejudice, the "racializing" power of political judgments, and the ideological dynamics of the Tea Party.

  5. Race, Ideology, and the Tea Party: A Longitudinal Study

    Science.gov (United States)

    Knowles, Eric D.; Lowery, Brian S.; Shulman, Elizabeth P.; Schaumberg, Rebecca L.

    2013-01-01

    The Tea Party movement, which rose to prominence in the United States after the election of President Barack Obama, provides an ideal context in which to examine the roles of racial concerns and ideology in politics. A three-wave longitudinal study tracked changes in White Americans’ self-identification with the Tea Party, racial concerns (prejudice and racial identification), and ideologies (libertarianism and social conservatism) over nine months. Latent Growth Modeling (LGM) was used to evaluate potential causal relationships between Tea Party identification and these factors. Across time points, racial prejudice was indirectly associated with movement identification through Whites’ assertions of national decline. Although initial levels of White identity did not predict change in Tea Party identification, initial levels of Tea Party identification predicted increases in White identity over the study period. Across the three assessments, support for the Tea Party fell among libertarians, but rose among social conservatives. Results are discussed in terms of legitimation theories of prejudice, the “racializing” power of political judgments, and the ideological dynamics of the Tea Party. PMID:23825630

  6. Race, ideology, and the tea party: a longitudinal study.

    Directory of Open Access Journals (Sweden)

    Eric D Knowles

    Full Text Available The Tea Party movement, which rose to prominence in the United States after the election of President Barack Obama, provides an ideal context in which to examine the roles of racial concerns and ideology in politics. A three-wave longitudinal study tracked changes in White Americans' self-identification with the Tea Party, racial concerns (prejudice and racial identification, and ideologies (libertarianism and social conservatism over nine months. Latent Growth Modeling (LGM was used to evaluate potential causal relationships between Tea Party identification and these factors. Across time points, racial prejudice was indirectly associated with movement identification through Whites' assertions of national decline. Although initial levels of White identity did not predict change in Tea Party identification, initial levels of Tea Party identification predicted increases in White identity over the study period. Across the three assessments, support for the Tea Party fell among libertarians, but rose among social conservatives. Results are discussed in terms of legitimation theories of prejudice, the "racializing" power of political judgments, and the ideological dynamics of the Tea Party.

  7. 77 FR 16036 - Guidance for Industry, Third Parties and Food and Drug Administration Staff; Medical Device ISO...

    Science.gov (United States)

    2012-03-19

    ... HUMAN SERVICES Food and Drug Administration Guidance for Industry, Third Parties and Food and Drug...--Requirements for regulatory purposes,'' (ISO 13485:2003) audit report provides FDA a degree of assurance of... GHTF founding members auditing systems include: The Canadian Medical Devices Conformity...

  8. Server-Aided Two-Party Computation with Simultaneous Corruption

    DEFF Research Database (Denmark)

    Cascudo Pueyo, Ignacio; Damgård, Ivan Bjerre; Ranellucci, Samuel

    We consider secure two-party computation in the client-server model where there are two adversaries that operate separately but simultaneously, each of them corrupting one of the parties and a restricted subset of servers that they interact with. We model security via the local universal composab...... composability framework introduced by Canetti and Vald and we show that information-theoretically secure two-party computation is possible if and only if there is always at least one server which remains uncorrupted....

  9. Direct and indirect welfare chauvinism as party strategies

    DEFF Research Database (Denmark)

    Careja, Romana; Elmelund-Præstekær, Christian; Klitgaard, Michael Baggesen

    2016-01-01

    and deliberate policy-making strategies of the party. It shows that the distinction between direct and indirect chauvinism is a useful theoretical tool for understanding how the Danish People's Party can fulfill the expectations of both its electorate and its coalition partners, even if they point in different......This article develops a theoretical distinction between direct and indirect welfare chauvinism in order to analyze how electorally successful populist right-wing parties transmit social policy preferences with significant redistributive implications for the shape of the welfare state. Direct...... directions....

  10. Multi-party arbitration in international trade: problems and solutions

    DEFF Research Database (Denmark)

    Siig, Kristina

    2007-01-01

    Legal disputes regarding international trade frequently involve more than two parties. This leads to problems, as the preferred means of dispute resolution within international trade - arbitration - tends to be ill-equipped to handle such disputes. The topic of the paper is arbitration as a means...... of dispute resolution in a multy-party set-up. Both the possible legal bases and the problems encountered are considere. It is concluded that arbitration is still the only real option to the parties in international business disputes and that many of the shortcomings  may be contered by skilful drafting...... of the arbitration agreement...

  11. Economic Conditions affect Support for Prime Minister Parties in Scandinavia

    DEFF Research Database (Denmark)

    Larsen, Martin Vinæs

    2016-01-01

    between unemployment, economic growth and support for prime minister parties is re-examined in two datasets. The first is a dataset of Scandinavian elections, and the second is a yearly Danish vote function, which was constructed using election polls. Across both datasets, it is found that if one simply...... correlates support for the prime minister's party with economic conditions, there is no relationship; however, if one specifies a statistical model, which takes the Scandinavian context into account, it is possible to identify a statistically significant effect of economic conditions on electoral support....... Based on this finding, the article concludes that economic conditions do shape electoral support for prime minister parties in Scandinavia....

  12. Role of political parties in the formation of civil society in Uzbekistan

    Directory of Open Access Journals (Sweden)

    Сирожжон Насипкулович Бердикулов

    2015-06-01

    Full Text Available The article considers the role of political parties in the development of civil society and the rule of law, as well as issues of political parties differ from other public organizations, the concept of a multi-party system, the importance of a multi-party system in ensuring political pluralism, inter-party competition, constructive opposition, the expression of the interests of the electorate, the space of political parties in parliament and local councils (soviets

  13. Quantum secret sharing between m-party and n-party with six states

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    A quantum secret sharing scheme between an m-party group and an n-party group is proposed using three conjugate bases.A sequence of single photons,each of which is prepared in one of the six states,is used directly to encode classical information in the quantum secret sharing process.In this scheme,each of all m members in group 1 chooses randomly his/her own secret key individually and independently,and directly encodes his/her respective secret information on the states of single photons via unitary operations,then the last one sends 1/n of the resulting qubits to each member of group 2.By measuring their respective qubits,all members in group 2 share the secret information shared by all members in group 1.It renders impossible a Trojan horse attack with a multi-photon signal,a fake-signal attack with EPR pairs,an attack with single photons,and an attack with invisible photons.We give the upper bounds on the average success probabilities for dishonest agent eavesdropping encryption using the fake-signal attack with any two-particle entangled states.

  14. 16 CFR 1209.40 - Certification responsibility, multiple parties.

    Science.gov (United States)

    2010-01-01

    ... SAFETY ACT REGULATIONS INTERIM SAFETY STANDARD FOR CELLULOSE INSULATION Certification § 1209.40... cellulose insulation, only the party closest to the consumer in the distribution chain is required to issue...

  15. Race, ideology, and the tea party: a longitudinal study

    National Research Council Canada - National Science Library

    Knowles, Eric D; Lowery, Brian S; Shulman, Elizabeth P; Schaumberg, Rebecca L

    2013-01-01

    The Tea Party movement, which rose to prominence in the United States after the election of President Barack Obama, provides an ideal context in which to examine the roles of racial concerns and ideology in politics...

  16. African Ruling Political Parties and the Making of 'Authoritarian ...

    African Journals Online (AJOL)

    An earlier version of this paper was presented at the Global Social Justice in Theory and ... African Ruling Political Parties and the Making of 'Authoritarian' Democracies .... Achieving social justice (particularly in its philosophical sense) has.

  17. The 2014 Elections and the Brazilian Party System

    Directory of Open Access Journals (Sweden)

    Carlos Ranulfo Melo

    2015-04-01

    Full Text Available The Brazilian party system presents a paradox. Although the Workers Party (PT and the Brazilian Social Democratic Party (PSDB have controlled presidential elections for the last 20 years, their force at the other levels of electoral competition has not grown. The objective of this study is to undertake a discussion of this situation through the 2014 general elections. After attempting to explain why successive challengers have not been able to change the structure of competition for the Brazilian presidency, I will analyze the relation established between the pattern observed at this level and the other “connected” electoral disputes—both those for state executive and federal legislative office. The general conclusion is that even if the pattern continues, it is quite improbable that this will significantly impact the other levels of national political party competition.

  18. Political Parties and Social Policy Responses to Global Economic Crises

    DEFF Research Database (Denmark)

    Starke, Peter; Kaasch, Alexandra; van Hooren, Franca

    2014-01-01

    responses, albeit in ways that go beyond traditional partisan theory.We show that the partisan conflict and the impact of parties are conditioned by existing welfare state configurations. In less generous welfare states, the party composition of governments plays a decisive role in shaping the direction...... to global economic crises vary significantly across countries. What explains the cross-national and within-case variation in responses to crises?We discuss several potential causes of this pattern and argue that political parties and the party composition of governments can play a key role in shaping crisis...... of social policy change. By contrast, in more generous welfare states, i.e., those with highly developed automatic stabilisers, the overall direction of policy change is regularly not subject to debate. Political conflict in these welfare states rather concerns the extent to which expansion or retrenchment...

  19. Alcohol Can Be a Risky Guest At Holiday Parties

    Science.gov (United States)

    ... page: https://medlineplus.gov/news/fullstory_162488.html Alcohol Can Be a Risky Guest at Holiday Parties ... a part of the festivities. Unfortunately, so are alcohol-related accidents and deaths. Many people who attend ...

  20. Party Nationalization and Electoral Strategies in Coalition Presidentialism

    National Research Council Canada - National Science Library

    Andre Borges

    2015-01-01

    ... a gubernatorial coattail effect. While some parties are successful in mobilizing the national electorate through "presidentialization" other groups may be capable of obtaining votes nationally for reasons that are unrelated to the...

  1. Third Party Reproduction: Sperm, Egg, and Embryo Donation and Surrogacy

    Science.gov (United States)

    ... third-party reproduction” refers to the use of eggs , sperm , or embryos that have been donated by a ... the requisite screening and testing required of all egg and sperm donors. For embryos that are created specifically for ...

  2. Political Parties and Social Policy Responses to Global Economic Crises

    DEFF Research Database (Denmark)

    Starke, Peter; Kaasch, Alexandra; van Hooren, Franca

    2014-01-01

    Based on empirical findings froma comparative study onwelfare state responses to the four major economic shocks (the 1970s oil shocks, the early 1990s recession, the 2008 financial crisis) in four OECD countries, this article demonstrates that, in contrast to conventional wisdom, policy responses...... to global economic crises vary significantly across countries. What explains the cross-national and within-case variation in responses to crises?We discuss several potential causes of this pattern and argue that political parties and the party composition of governments can play a key role in shaping crisis...... responses, albeit in ways that go beyond traditional partisan theory.We show that the partisan conflict and the impact of parties are conditioned by existing welfare state configurations. In less generous welfare states, the party composition of governments plays a decisive role in shaping the direction...

  3. 47 CFR 76.935 - Participation of interested parties.

    Science.gov (United States)

    2010-10-01

    ... parties. In order to regulate basic tier rates or associated equipment costs, a franchising authority must..., 120, or 180-day time periods that franchising authorities have to review rates under § 76.933....

  4. ISLAMIC PARTIES AND THE POLITICS OF CONSTITUTIONALISM IN INDONESIA

    Directory of Open Access Journals (Sweden)

    Mukrimin Mukrimin

    2012-12-01

    Full Text Available This article examines the relationship between religion and the state in Indonesia by exploring how Islam is used by political parties to shape the politics. The study shows that Islamic politics is a complementary in both nationhood and statehood in Indonesia. From the early days of the new-born nation-state, Muslims in Indonesia had played significant role in shaping the nation; nonetheless, they have never dominated the political power. Fragmentation among Muslims themselves and internal political parties is among the reason why religious (Islamic parties failed in bringing religious identity to the state arena. Political subordination-inclusion-ignorance-confrontation is the circular game that features Islamic politics in Indonesia. The debatable issue on shari‘ah law, which is frequently used by Islamic parties, always becomes the core problem of the relation between Islam and the state. Even though Islam has played an important role in colouring the Indonesian politics, its existence remains complementary.

  5. Music: An Oral History of All Tomorrow's Parties

    National Research Council Canada - National Science Library

    Weingarten, Christopher R

    2009-01-01

    A discussion recalling the history of the All Tomorrow's Parties (ATP) indie music festival, which will be held in New York for the second time beginning September 11, 2009, at Kutshers Country Club in Monticello, is presented...

  6. Contextualizing Intergroup Contact: Do Political Party Cues Enhance Contact Effects?

    DEFF Research Database (Denmark)

    Sønderskov, Kim Mannemar; Thomsen, Jens Peter Frølund

    2015-01-01

    political parties on us-them categorizations heightens the awareness of group memberships. This focus in turn enhances the positive intergroup contact effect by stimulating majority members to perceive contacted persons as prototypical outgroup members. A multilevel analysis of 22 countries and almost 37......,000 individuals confirms that the ability of intergroup contact to reduce antiforeigner sentiment increases when political parties focus intensively on immigration issues and cultural differences. Specifically, both workplace contact and interethnic friendship become more effective in reducing antiforeigner...

  7. Social media as political party campaign in Indonesia

    OpenAIRE

    Abdillah, Leon Andretti

    2014-01-01

    Social media as a trend in the Internet is now used as a medium for political campaigns. Author explores the advantages and social media implementation of any political party in Indonesia legislative elections 2014. Author visited and analyzed social media used by the contestants, such as: Facebook, and Twitter. Author collected data from social media until the end of April 2014. This article discusses the use of social media by political parties and their features. The results of this study ...

  8. The Workers’ Party and the political use of judiciary

    Directory of Open Access Journals (Sweden)

    Matthew M. TAYLOR

    2011-01-01

    Full Text Available This paper draws on the experience of the Brazilian Workers’ Party (PT, during periods of opposition and incumbency, to develop a theoretical approach to the use of Courts by political actors. I argue that the use of Courts by political parties is an outcome of their relations with executive incumbents, the institutional architecture of the judiciary, and a strategic evaluation of the different judicial mechanisms that are available to them.

  9. [Susceptibilities of multidrug-resistant pathogens responsible for complicated skin and soft tissue infections to standard bacteriophage cocktails].

    Science.gov (United States)

    Gündoğdu, Aycan; Kılıç, Hüseyin; Ulu Kılıç, Ayşegül; Kutateladze, Mzia

    2016-04-01

    Skin and soft tissue infections (SSTIs) may represent a wide clinical spectrum from cellulitis to high-mortality associated necrotizing fasciitis. Limitations in therapy due to the multiple drug resistance, leads to increase in the morbidity and mortality rates, especially in complicated SSTIs such as diabetic foot, decubitus, and surgical wound infections. Therefore, alternative treatment strategies other than antibiotics are needed in appropriate clinical conditions. "Bacteriophage therapy", which is an old method and has been used as part of standard treatment in some countries such as Georgia and Russia, has again become popular worldwide. The aim of this study was to investigate the in vitro susceptibilities of multidrug-resistant (MDR) pathogens isolated from patients with complicated SSTIs, against standard bacteriophage (phage) cocktails. Six different ready-made phage preparations [Pyophage, Intestiphage, ENKO, SES, Fersisi and Staphylococcal Bacteriophage (Sb)] used in this study have been provided by G. Eliava Institute, Georgia. Because of the absence of ready-made phage preparations for Acinetobacter baumannii and Klebsiella pneumoniae, Φ1-Φ7 and ΦKL1- ΦKL3 phages were used provided from the same institute's phage library, respectively. Isolation and identification of the pathogens from abscess and wound samples of patients with SSTIs were performed by conventional methods and automatized VITEK(®)-2 (bioMerieux, ABD) system. Antimicrobial susceptibility testing was conducted complying CLSI standards' and the bacteria that were resistant to at least two different antibiotic groups were considered as MDR. Accordingly, a total of 33 isolates, nine of them were E.coli (8 ESBL and 1 ESBL + carbapenemase positive); nine were MDR P.aeruginosa; nine were MDR A.baumannii; three were methicillin-resistant Staphylococcus aureus (MRSA) and three were K.pneumoniae (1 ESBL, 1 carbapenemase and 1 ESBL + carbapenemase positive) were included in the study. The

  10. Modern Management, Individualization and Maintenance of Collective Interests: A Complex Cocktail

    DEFF Research Database (Denmark)

    Andersen, Vibeke

    2003-01-01

    One of the objectives of modern management is to mobilize and develop human ressources while simultaneously sharing responsibility with employees, thus, giving them a chance to actively contribute to the development of their work in a way that promotes flexibility, effiency and quality. This idea...

  11. Comparison of Radical Right-Wing Parties in Bulgaria and Romania: The National Movement of Ataka and the Great Romania Party

    Directory of Open Access Journals (Sweden)

    Markéta Smrčková

    2009-04-01

    Full Text Available This paper focuses on a comparison of two radical right-wing parties, Ataka and the Great Romania Party, their positions in the national party system, and their relationships with the governing parties as well as with other national right-wing parties. In this essay the party programmes, electorate, attitude to minorities, and degree of nationalism will be compared. Another important part of this paper is comparison of the election results of these parties and their chances of remaining in the national parliaments (and thus becoming a crucial part of party system. All the details mentioned above will be analyzed against the background of theories of radical right-wing parties.

  12. PRODUCTION OF FERMENTABLE SUGARS FROM OIL PALM EMPTY FRUIT BUNCH USING CRUDE CELLULASE COCKTAILS WITH TRICHODERMA ASPERELLUM UPM1 AND ASPERGILLUS FUMIGATUS UPM2 FOR BIOETHANOL PRODUCTION

    Directory of Open Access Journals (Sweden)

    Nurul Kartini Abu Bakar,

    2012-06-01

    Full Text Available Utilization of oil palm empty fruit bunch (OPEFB for bioethanol production with crude cellulase cocktails from locally isolated fungi was studied. Enzymatic saccharification of alkaline pretreated OPEFB was done using different cellulase enzyme preparations. Crude cellulase cocktails from Trichoderma asperellum UPM1 and Aspergillus fumigatus UPM2 produced 8.37 g/L reducing sugars with 0.17 g/g yield. Production of bioethanol from OPEFB hydrolysate using Baker’s yeast produced approximately 0.59 g/L ethanol, corresponding to 13.8% of the theoretical yield. High reducing sugars concentration in the final fermentation samples resulted from accumulation of non-fermentable sugars such as xylose and cellobiose that were not consumed by the yeast. The results obtained support the possible utilization of OPEFB biomass for bioethanol production in the future.

  13. The effects of third-party validation and minimization on judgments of the transgressor and the third party.

    Science.gov (United States)

    Eaton, Judy

    2013-06-01

    Victims of interpersonal transgressions often turn to friends, family, and trusted others when trying to make sense of negative events. This research explored the effect of two of the many ways that these informal third parties can respond: validating the victim's experience, and downplaying or minimizing the transgression. Two studies found that validation from a third party increases revenge motivations and that minimization of the transgression is more effective than validation at reducing revenge motivations. However, results also indicated that victims judge third parties more positively if they validate rather than minimize the transgression. These findings suggest that, when choosing between validation and minimization, third parties must make a choice between promoting forgiveness and promoting themselves.

  14. Formulation of a Medical Food Cocktail for Alzheimer's Disease: Beneficial Effects on Cognition and Neuropathology in a Mouse Model of the Disease

    OpenAIRE

    2010-01-01

    BACKGROUND: Dietary supplements have been extensively studied for their beneficial effects on cognition and AD neuropathology. The current study examines the effect of a medical food cocktail consisting of the dietary supplements curcumin, piperine, epigallocatechin gallate, α-lipoic acid, N-acetylcysteine, B vitamins, vitamin C, and folate on cognitive functioning and the AD hallmark features and amyloid-beta (Aβ) in the Tg2576 mouse model of the disease. PRINCIPAL FINDINGS: The study found ...

  15. Using Third-Party Inspectors in Building Energy Codes Enforcement in India

    Energy Technology Data Exchange (ETDEWEB)

    Yu, Sha; Evans, Meredydd; Kumar, Pradeep; Van Wie, Laura; Bhatt, Vatsal

    2013-01-31

    India is experiencing fast income growth and urbanization, and this leads to unprecedented increases in demand for building energy services and resulting energy consumption. In response to rapid growth in building energy use, the Government of India issued the Energy Conservation Building Code (ECBC) in 2007, which is consistent with and based on the 2001 Energy Conservation Act. ECBC implementation has been voluntary since its enactment and a few states have started to make progress towards mandatory implementation. Rajasthan is the first state in India to adopt ECBC as a mandatory code. The State adopted ECBC with minor additions on March 28, 2011 through a stakeholder process; it became mandatory in Rajasthan on September 28, 2011. Tamil Nadu, Gujarat, and Andhra Pradesh have started to draft an implementation roadmap and build capacity for its implementation. The Bureau of Energy Efficiency (BEE) plans to encourage more states to adopt ECBC in the near future, including Haryana, Uttar Pradesh, Karnataka, Maharashtra, West Bengal, and Delhi. Since its inception, India has applied the code on a voluntary basis, but the Government of India is developing a strategy to mandate compliance. Implementing ECBC requires coordination between the Ministry of Power and the Ministry of Urban Development at the national level as well as interdepartmental coordination at the state level. One challenge is that the Urban Local Bodies (ULBs), the enforcement entities of building by-laws, lack capacity to implement ECBC effectively. For example, ULBs in some states might find the building permitting procedures to be too complex; in other cases, lack of awareness and technical knowledge on ECBC slows down the amendment of local building by-laws as well as ECBC implementation. The intent of this white paper is to share with Indian decision-makers code enforcement approaches: through code officials, third-party inspectors, or a hybrid approach. Given the limited capacity and human

  16. Development of a Novel Cocktail Enzyme-Linked Immunosorbent Assay and a Field-Applicable Lateral-Flow Rapid Test for Diagnosis of Contagious Bovine Pleuropneumonia.

    Science.gov (United States)

    Heller, Martin; Gicheru, Nimmo; Tjipura-Zaire, Georgina; Muriuki, Cecilia; Yu, Mingyan; Botelho, Ana; Naessens, Jan; Jores, Joerg; Liljander, Anne

    2016-06-01

    Contagious bovine pleuropneumonia (CBPP) is a severe respiratory disease that is widespread in sub-Saharan Africa. It is caused by Mycoplasma mycoides subsp. mycoides, a bacterium belonging to the Mycoplasma mycoides cluster. In the absence of an efficient CBPP vaccine, improved and easy-to-use diagnostic assays for recurrent testing combined with isolation and treatment of positive animals represent an option for CBPP control in Africa. Here we describe the comprehensive screening of 17 immunogenic Mycoplasma mycoides subsp. mycoides proteins using well-characterized bovine sera for the development of a novel cocktail enzyme-linked immunosorbent assay (ELISA) for laboratory use. Two recombinant Mycoplasma immunogens, MSC_0136 and MSC_0636, were used to set up a standardized cocktail ELISA protocol. According to the results from more than 100 serum samples tested, the sensitivity and specificity of the novel cocktail ELISA were 85.6% and 96.4%, respectively, with an overall diagnostic accuracy comparable to that of the Office International des Epizooties (OIE)-prescribed serological assays. In addition, we provide a proof of principle for a field-applicable, easy-to-use commercially produced prototype lateral-flow test for rapid (<30-min) diagnosis of CBPP.

  17. "Public Purpose or Public Interest" and Third Party Transfers

    Directory of Open Access Journals (Sweden)

    Bradley Virgill

    2014-04-01

    Full Text Available In this article the difference between public purpose and public interest in section 25(2 of the 1996 Constitution is considered. It is generally accepted that public purpose is a narrower category than public interest and that the distinction between public purpose and public interest does not make any practical difference. However, in this contribution it is suggested that the difference between public purpose and public interest makes no practical difference only in cases where expropriated property is used by the state for the realisation of a particular purpose. The difference between public purpose and public interest becomes more important when a particular expropriation also involves a third party transfer, since it indicates the level of scrutiny that the courts should apply in determining the lawfulness of the expropriation. When property is expropriated and transferred to a third party for the realisation of a public purpose, such as building and managing electricity plants, the lawfulness of the expropriation is not easily questioned. As such, the application of a rationality test to determine the legitimacy of the expropriation is generally easy to accept. However, this lenient approach cannot be as easily accepted where an expropriation and third party transfer takes place in the public interest. Examples of third party transfers in the public interest include land reform, slum clearance and economic development. In the examples of land reform and slum clearance the expropriation and third party transfer is usually authorised in legislation or, as is the case with land reform in South Africa, the 1996 Constitution. Because (as in the land reform example the expropriation and third party transfer is authorised by the Constitution and regulated by legislation, the application of a rationality test to determine the legitimacy is acceptable. However, the application of a rationality test where property is expropriated and transferred

  18. Hotel Overbooking and Cooperation with Third-Party Websites

    Directory of Open Access Journals (Sweden)

    Yufeng Dong

    2015-08-01

    Full Text Available Hotels cooperate with third-party websites to enhance their competitive position and attain sustainable development in the era of e-commerce. Furthermore, hotel managers overbook to hedge against last-minute cancellations and customer no-shows. This paper discusses pricing and overbooking strategies of a hotel in the context of cooperation with multiple third-party websites and analyzes how these strategies influence the cooperation process. The increase in profits resulting from the overbooking strategy is also examined. A model of a Stackelberg game between the hotel and third-party websites and a Nash game among the third-party websites are developed to analyze the process. Results indicate that the compensation coefficient has a slight influence on hotel pricing strategy, but causes a sharp decrease in overbooking level. As the hotel demands to lessen the probability of denying under higher compensation rate, the third-party websites would exert less sales effort to reduce the demand of online customers. Results also show that under a given market demand, hotel effort only serves to redistribute market shares among the hotel and third-party websites, without influencing hotel pricing and overbooking decisions. Specifically, the market shares of the third-party websites decrease in the effort level of the hotel resulting from an increase in the hotel market share. Finally, in the numerical example where one hotel and two websites are analyzed, hotel overbooking strategy improves hotel profit by 4.20%, whereas profit improvements for Websites 1 and 2 are as high as 5.26% and 5.21%, respectively. Managerial implications of the study are also provided.

  19. The effect of increased levels of dried coconut meal supplemented with an enzyme cocktail® on diet utilization in growing pigs

    Directory of Open Access Journals (Sweden)

    Prawit Rodjan

    2017-02-01

    Full Text Available The experiment was conducted to determine the effect of increased levels of dried coconut meal supplemented with an enzyme cocktail® on diet utilization in growing pigs. A 4x4 latin square design was used in this study. Four crossbred (Duroc x Landrace x Large White barrows averaging 17.88±0.96 kg in body weight were allotted 4 diets, diet 1 (the control diet, diet 2, diet 3 and diet 4 (5, 10 and 15% dried coconut meal in each diet with an enzyme cocktail® , 1 kg of the enzyme cocktail containing the activities of phytase 1,000,000 units, amylase 5,000,000 units, xylanase 3,500,000 units, beta-glucanase 2,000,000 units, cellulase 1,500,000 units, pectinase 1,000,000 units and mannanase 800,000 units; 500 g/t of feed, at a level 0.05% in the diets, respectively. Pigs were raised in individual metabolism cages. Faeces and urine samples were collected 4 times a day for 5 days for data collection. The results showed that the nutrient digestibility percentage of dry matter, crude protein, crude fat, ash, nitrogen-free extract, blood urea nitrogen, digestible energy (kcal/kg and metabolizable energy (kcal/ kg were not significantly different (P>0.05 in pigs fed with different diets. However, pigs fed with 5, 10 and 15% dried coconut meal in the diet with an enzyme cocktail® at a level 0.05% had significantly (P0.05 from the other groups. Apparent biological values contrarily reduced (linear and quadratic, P<0.05 with increasing level of dried coconut meal in pig diets. In conclusion, our data indicate that pigs fed with 5% dried coconut meal in a diet with addition of an enzyme cocktail at a level of 0.05% can show obviously increasing the highest digestibility of crude fiber (79.25% without impairing nutrient digestibility.

  20. The gender of party leadership and its impact on voter's asessment

    DEFF Research Database (Denmark)

    Kosiara-Pedersen, Karina

    Even though most political parties are still male dominated, an increasing number of women reach the top position of party leader. Furthermore, party leadership is increasingly regarded as an important part of party images due to the personalization of politics. Does the gender of the party leade...... leader characteristics appreciated by men and women. If this holds, the gender of party leadership matters to women because they value woman party leaders higher, not because they value other characteristics than men.......Even though most political parties are still male dominated, an increasing number of women reach the top position of party leader. Furthermore, party leadership is increasingly regarded as an important part of party images due to the personalization of politics. Does the gender of the party leader....... However, men are not more sympathetic towards man party leaders than women. Second, the analysis of the content of party leader sympathy seems to show that men and women do not differ in the characteristics correlated with party leader sympathy. Hence, there seems to be no gender difference in the party...

  1. The power of populism. Geert Wilders and the Party for Freedom in the Netherlands

    NARCIS (Netherlands)

    Vossen, K.P.S.S.

    2017-01-01

    This book discusses the Party for Freedom (PVV), a political party in the Netherlands, founded and led by Geert Wilders. Attaining between 10 and 18% of the votes, the PVV has become one of the largest parties in the Netherlands and is the only political party worldwide without members. Between 2010

  2. The Role of Political Parties in Denmark, the United States, and Uganda

    DEFF Research Database (Denmark)

    Skaates, Maria Anne; Basajjabaka, Abubaker

    1996-01-01

    A comparative analysis of the role of political parties in Denmark, the USA, and Uganda on the basis of political science theories about parties, political participation and political systems.......A comparative analysis of the role of political parties in Denmark, the USA, and Uganda on the basis of political science theories about parties, political participation and political systems....

  3. The Power of Populism. Geert Wilders and the Party for Freedom in the Netherlands

    NARCIS (Netherlands)

    Vossen, K.P.S.S.

    2017-01-01

    This book discusses the Party for Freedom (PVV), a political party in the Netherlands, founded and led by Geert Wilders. Attaining between 10 and 18% of the votes, the PVV has become one of the largest parties in the Netherlands and is the only political party worldwide without members. Between 2010

  4. Cocktail 探针药物法评价辣椒素对大鼠CYP450亚型的影响%Effects of Capsaicin on Rat Cytochrome P450 Isoforms by Cocktail Probe Drug Method

    Institute of Scientific and Technical Information of China (English)

    舒舟; 翟学佳; 刘金梅; 吕永宁

    2014-01-01

    目的::采用cocktail 探针药物法研究辣椒素在体外对大鼠肝微粒体CYP 450四种亚型的影响。方法:分为试验组和对照组,试验组采用大鼠肝微粒体孵育体系、探针药物和系列浓度的辣椒素(对照组仅加入缓冲液)共同孵育20 min,反应终止后用HPLC方法检测代谢产物的生成量以代表酶的活性。采用Graphpad prism 5.0计算辣椒素对各亚型的IC50值。将辣椒素与大鼠肝微粒体分别预孵育0,5,10,15,20,30 min,计算不同预孵育时间下各亚型的相对活性百分比。结果:辣椒素对大鼠肝微粒体CYP1A2、CYP2C11、CYP2E1和CYP3A2的IC50值分别为36.21,17.19,51.64,18.86μmol·L-1。预孵育没有增强辣椒素对CYP450的抑制作用。结论:辣椒素在体外对大鼠肝微粒CYP1A2、CYP2C11、CYP2E1和CYP3A2具有抑制作用,且未呈现出预孵育时间依赖性。%Objective:To investigate the in vitro effect of capsaicin on four major rat cytochrome P450 ( CYP) enzymes using a cocktail probe drug method. Methods:The in vitro incubation was divided into capsaicin group and the control group. Rat liver micro-somes, probe drugs, capsaicin at various concentration ( buffer solution in the control group) and cofactors were cultured altogether for 20 min. After the culture, the concentration of metabolites was determined by HPLC to assess the activities of enzymes. IC50 value of capsaicin on every isoform was calculated using Graphpad prism 5. 0. Capsaicin and hepatic microsomess were pre-incubated respec-tively for 0, 5, 10, 15, 20 and 30 min, and then the relative activity of the four isoforms at different time was calculated. Results:The activity of the rat liver microsomes enzyme CYP450 isoforms (CYP1A2, CYP2C11, CYP2E1 and CYP3A2) was all inhibited by capsaicin in vitro with IC50 value of 36. 21, 17. 19, 51. 64 and 18. 86 μmol·L-1 , respectively. Pre-incubation could not increase cap-saicin inhibitory activity against the four CYP enzymes

  5. Profitability, third-party reimbursement, and access to community pharmacies.

    Science.gov (United States)

    Carroll, N V; Miederhoff, P A; Waters, L W

    1996-01-01

    The purpose of this study was to analyze the extent to which third-party reimbursement programs have affected the profitability and availability of community pharmacies. Data were taken from records maintained by the Virginia Board of Pharmacy and a survey of 177 community pharmacies. Between 1989 and 1994, 258 outpatient pharmacies opened and 342 closed. Chain and independent pharmacies suffered net losses, and supermarket and mass merchandiser pharmacies experienced net increases. Few significant changes occurred in the distribution of pharmacies over the study period. Fifty-nine chain and independent pharmacies and 1 supermarket pharmacy chain provided usable profit and reimbursement data. These pharmacies experienced declines in profits and increases in the percentage of prescriptions reimbursed by private third-party prescription programs over the last several years. Regression analyses indicated that higher ratios of sales of private third-party prescriptions to private-pay prescriptions were associated with lower profits. All respondents indicated that changes in private third-party reimbursement had substantially reduced profits over the past 5 years. The results indicate that the growth of private third-party payment has led to lower pharmacy profits but has not yet resulted in problems of consumer access.

  6. Postconflict third-party affiliation in rooks, Corvus frugilegus.

    Science.gov (United States)

    Seed, Amanda M; Clayton, Nicola S; Emery, Nathan J

    2007-01-23

    Conflict features in the lives of many animal species and induces social stress mediated by glucocorticoid hormones [1]. Postconflict affiliation, between former opponents (reconciliation) or between former opponents and a bystander (third-party affiliation), has been suggested as a behavioral mechanism for reducing such stress [2], but has been studied almost exclusively in primates [3]. As with many primates, several bird species live in social groups and form affiliative relationships [4]. Do these distantly related animals also use affiliative behavior to offset the costs of conflict? We studied postconflict affiliation in a captive group of rooks. Unlike polygamous primates, monogamous rooks did not reconcile with former opponents. However, we found clear evidence of third-party affiliation after conflicts. Both initiators and targets of aggression engaged in third-party affiliation with a social partner and employed a specific behavior, bill twining, during the postconflict period. Both former aggressors and uninvolved third parties initiated affiliative contacts. Despite the long history of evolutionary divergence, the pattern of third-party affiliation in rooks is strikingly similar to that observed in tolerant primate species. Furthermore, the absence of reconciliation in rooks makes sense in light of the species differences in social systems.

  7. Peoples Democratic Party in the Fourth Republic of Nigeria

    Directory of Open Access Journals (Sweden)

    Aliyu Mukhtar Katsina

    2016-06-01

    Full Text Available Their nature and functions make political parties central to democratic governance especially in the new democracies of Africa that face the challenge of building strong and enduring democratic institutions. It is accepted that the existing trend in most of these democracies of one big party dominating the political space weakens democracy and undermines its prospects for consolidation. Big parties—usually the ruling ones—exhibit tendencies such as absence of internal democracy that are antithetical to democratic governance. While observations such as these are incontestable, there is little understanding into the nature, character, ideology, and internal structure of big parties generally. In this article, I attempt to address this concern. Specifically, I examine the nature, structure, and ideology of the Peoples Democratic Party (PDP, Nigeria’s ruling party at the federal level with considerable strength at local level between 1999 and 2015. Relying on data obtained from multiple sources, I investigate the process of its formation, the nature of its ideology, internal organization, its electoral strength, and how absence of internal democracy contributed significantly to its defeat in 2015 general elections.

  8. Ideology, Party Systems and Corruption Voting in European Democracies

    DEFF Research Database (Denmark)

    Charron, Nicholas; Bågenholm, Andreas

    2016-01-01

    What is the impact of corruption on citizens' voting behavior? There is a growing literature on an increasingly ubiquitous puzzle in many democratic countries: that corrupt officials continue to be re-elected by voters. In this study we address this issue with a novel theory and newly collected...... original survey data for 24 European countries. The crux of the argument is that voters' ideology is a salient factor in explaining why citizens would continue voting for their preferred party despite the fact that it has been involved in a corruption scandal. Developing a theory of supply (number...... of effective parties) and demand (voters must have acceptable ideological alternatives to their preferred party), we posit that there is a U-shaped relationship between the likelihood of corruption voting and where voters place themselves on the left/right spectrum. The further to the fringes, the more likely...

  9. Product market competition, ultimate controlling structure and related party transactions

    Directory of Open Access Journals (Sweden)

    Shenglan Chen

    2012-12-01

    Full Text Available Previous studies have shown that product market competition has an important effect on corporate strategies and internal governance mechanisms. Using a sample of China’s listed firms from 2004 to 2009, we explore the relationship between product market competition and normal related party transactions and find a significant positive relationship. In addition, we investigate the substitutive effect of product market competition and the cash flow rights owned by ultimate controlling shareholders on the extent of normal related party transactions. In particular, our results suggest a positive relationship between the ultimate controlling shareholders’ cash flow rights and normal related party transactions that is strongest in noncompetitive industries and weakens as product market competition increases.

  10. JCFA New President Koichi Kato and His Party in China

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    <正>Invited by the China-Japan Friendship Association (CJFA), a 11-member delegation of the Japan-China Friendship Association (JCFA) headed by Koichi Kato, new president of the JCFA and former secretary general of Japan’s Liberal Democratic Party, visited China from September 5 to 7, during which they attended the opening ceremony of the Beijing Paralympic Games and other relevant activities. President Hu Jintao met with Koichi Kato and his party. Koichi Kato attended the welcoming banquet President Hu Jintao and his wife hosted in honour of the dignitaries from various countries who had come to China to watch the Paralympics. Koichi Kato and his party made an official call on the Ministry of Foreign Affairs and the Central Committee of the Communist Youth League of China and had a working meeting with the CJFA.

  11. The Romanian Social Democratic Party versus the authoritarian monarchy

    Directory of Open Access Journals (Sweden)

    Florin Grecu

    2014-09-01

    Full Text Available The article approaches the Romanian social-democratic collaboration during 1938-1940 with the authoritarian monarch regime. Even though the party leaders had diverging political views, regards to the acceptance or the non-acceptance of the authoritarian regime, the influential PSDR members held leading positions within the single party and the corporate parliament and within the union structures. The positions were offered by the regime, so that the union leaders would stop instigating workers to go on strike, and to accept the governmental policies. The freedom of speech and the political actions were ceded to the monarch, who governed at the place of the political parties and he controlled the unions, by using the guilds.

  12. THE THIRD PARTY CERTIFICATION SYSTEM FOR ORGANIC PRODUCTS

    Directory of Open Access Journals (Sweden)

    Adina-Roxana MUNTEANU

    2015-12-01

    Full Text Available In the context of an increasing organic trade worldwide, the organic standards and the certification of the organic practices are ever more important and relevant for both business and consumers. The certification by third parties of the organic operators is meant to ensure the application of an organic standard. However, the documented fraud cases are proof that the current system can undergo further improvement. The current paper gives an overview of the mechanisms of certification and inspection worldwide and discusses the issues raised by third party certification of the organic operators in the European context. Moreover, the paper reviews the major arguments for the effectiveness of the third party certification and also points to the improvement possibilities of the systems.

  13. The risky cocktail: what combination effects can we expect between ecstasy and other amphetamines?

    Science.gov (United States)

    Dias da Silva, Diana; Carmo, Helena; Silva, Elisabete

    2013-01-01

    The recreational and illicit use of amphetaminic designer compounds, specially 3,4-methylenedioxymethamphetamine (MDMA; Ecstasy), is of concern worldwide. Such psychostimulating drugs are frequently present as complex mixtures in 'rave' pills, making concomitant polysubstance use a common trend. However, the understanding of possible combination effects with these substances is still scarce. The present study was aimed at predicting the cytotoxic effects of mixtures of four amphetaminic derivatives: MDMA, methamphetamine, 4-methylthioamphetamine and d-amphetamine in a human hepatoma cell line. Concentration-response curves for all single-mixture components were recorded by the MTT assay. Data obtained for individual agents were then used to compute the additivity expectations for mixtures of definite composition, using the pharmacological models of concentration addition (CA) and independent action. By comparing the predicted calculations with the experimentally observed effects, we concluded that CA accurately predicts the combination of amphetamines, which act together to generate additive effects over a large range of concentrations. Notably, we observed substantial mixture effects even when each drug was present at low concentrations, which individually produced unnoticeable effects. Nonetheless, for all tested mixtures, a small deviation from additivity was observed towards higher concentrations, particularly at high effect levels. A possible metabolic interaction, which could explain such deviation, was investigated, and it was observed that at higher mixture concentrations increased MDMA metabolism could be contributing to divergences from additivity. In conclusion, the present work clearly demonstrates that potentially harmful interactions among amphetaminic drugs are expected when these drugs are taken concomitantly.

  14. Optimal immunization cocktails can promote induction of broadly neutralizing Abs against highly mutable pathogens.

    Science.gov (United States)

    Shaffer, J Scott; Moore, Penny L; Kardar, Mehran; Chakraborty, Arup K

    2016-10-24

    Strategies to elicit Abs that can neutralize diverse strains of a highly mutable pathogen are likely to result in a potent vaccine. Broadly neutralizing Abs (bnAbs) against HIV have been isolated from patients, proving that the human immune system can evolve them. Using computer simulations and theory, we study immunization with diverse mixtures of variant antigens (Ags). Our results show that particular choices for the number of variant Ags and the mutational distances separating them maximize the probability of inducing bnAbs. The variant Ags represent potentially conflicting selection forces that can frustrate the Darwinian evolutionary process of affinity maturation. An intermediate level of frustration maximizes the chance of evolving bnAbs. A simple model makes vivid the origin of this principle of optimal frustration. Our results, combined with past studies, suggest that an appropriately chosen permutation of immunization with an optimally designed mixture (using the principles that we describe) and sequential immunization with variant Ags that are separated by relatively large mutational distances may best promote the evolution of bnAbs.

  15. Cocktail treatment, a promising strategy to treat acute cerebral ischemic stroke?

    Directory of Open Access Journals (Sweden)

    Li-jun Liang

    2016-01-01

    Full Text Available Up to now, over 1,000 experimental treatments found in cells and rodents have been difficult to translate to human ischemic stroke. Since ischemia and reperfusion, two separate stages of ischemic stroke, have different pathophysiological mechanisms leading to brain injury, a combination of protective agents targeting ischemia and reperfusion respectively may obtain substantially better results than a single agent. Normobaric hyperoxia (NBO has been shown to exhibit neuro- and vaso-protective effects by improving tissue oxygenation when it is given during ischemia, however the effect of NBO would diminish when the duration of ischemia and reperfusion was extended. Therefore, during reperfusion drug treatment targeting inflammation, oxidative stress and free radical scavenger would be a useful adjuvant to extend the therapeutic window of tissue plasminogen activator, the only United States Food and Drug Administration (FDA approved treatment for acute ischemic stroke. In this review, we discussed the neuro- and vaso-protective effects of NBO and recent finding of combining NBO with other drugs.

  16. Cocktail of Superoxide Dismutase and Fasudil Encapsulated in Targeted Liposomes Slows PAH Progression at a Reduced Dosing Frequency.

    Science.gov (United States)

    Gupta, Nilesh; Rashid, Jahidur; Nozik-Grayck, Eva; McMurtry, Ivan F; Stenmark, Kurt R; Ahsan, Fakhrul

    2017-03-06

    Currently, two or more pulmonary vasodilators are used to treat pulmonary arterial hypertension (PAH), but conventional vasodilators alone cannot reverse disease progression. In this study, we tested the hypothesis that a combination therapy comprising a vasodilator plus a therapeutic agent that slows pulmonary arterial remodeling and right heart hypertrophy is an efficacious alternative to current vasodilator-based PAH therapy. Thus, we encapsulated a cocktail of superoxide dismutase (SOD), a superoxide scavenger, and fasudil, a specific rho-kinase inhibitor, into a liposomal formulation equipped with a homing peptide, CAR. We evaluated the effect of the formulations on pulmonary hemodynamics in monocrotaline-induced PAH rats (MCT-induced PAH) and assessed the formulation's efficacy in slowing the disease progression in Sugen-5416/hypoxia-induced PAH rats (SU/hypoxia-induced PAH). For acute studies, we monitored both mean pulmonary and systemic arterial pressures (mPAP and mSAP) for 2 to 6 h after a single dose of the plain drugs or formulations. In chronic studies, PAH rats received plain drugs every 48 h and the formulations every 72 h for 21 days. In MCT-induced PAH rats, CAR-modified liposomes containing fasudil plus SOD elicited a more pronounced, prolonged, and selective reduction in mPAP than unmodified liposomes and plain drugs did. In SU/hypoxia-induced PAH rats, the formulation produced a >50% reduction in mPAP and slowed right ventricular hypertrophy. When compared with individual plain drugs or combination, CAR-modified-liposomes containing both drugs reduced the extent of collagen deposition, muscularization of arteries, increased SOD levels in the lungs, and decreased the expression of pSTAT-3 and p-MYPT1. Overall, CAR-modified-liposomes of SOD plus fasudil, given every 72 h, was as efficacious as plain drugs, given every 48 h, suggesting that the formulation can reduce the total drug intake, systemic exposures, and dosing frequency.

  17. Routine dual-color immunostaining with a 3-antibody cocktail improves the detection of small cancers in prostate needle biopsies.

    Science.gov (United States)

    Tolonen, Teemu T; Kujala, Paula M; Laurila, Marita; Tirkkonen, Mika; Ilvesaro, Joanna; Tuominen, Vilppu J; Tammela, Teuvo L J; Isola, Jorma

    2011-11-01

    We performed dual-color immunostaining with a 3-antibody cocktail (α-methylacyl coenzyme-A racemase, CK34betaE12, and p63) on prostate biopsies from 200 patients. Current practice (hematoxylin and eosin staining followed by dual-color immunostaining on selected cases) was compared with a protocol in which routine dual-color immunostaining was provided in all cases. In the original pathology reports, adenocarcinoma was diagnosed in 87/200 (43%) patients. Small foci interpreted as putative cancers were detected with dual-color immunostaining in 14/113 patients who were originally diagnosed with a nonmalignant lesion. All of the suggested cancerous foci were independently reevaluated by 5 pathologists. A diagnosis of adenocarcinoma was assessed by consensus in 8 cases, and atypical small acinar proliferation was diagnosed in 1 case. Consensus was not reached in 5 cases. Six of the foci reclassified as cancer were of Gleason score 3 + 3 = 6, while 2 were graded as Gleason score 4 + 4 = 8. The feasibility of routine dual-color immunostaining was also tested by analyzing the time spent on microscopic assessment. Because small, atypical lesions expressing α-methylacyl coenzyme-A racemase (blue chromogen) were easy to detect using dual-color immunostaining, the microscopic analysis of dual-color immunostaining and hematoxylin-eosin staining was faster than that of hematoxylin-eosin staining alone that was later followed by dual-color immunostaining in selected cases (median 251 seconds versus 299 seconds, P < .0001). We concluded that routine dual-color immunostaining of all prostate biopsies would produce better diagnostic sensitivity with a smaller microscopy workload for the pathologist. However, minute foci interpreted as cancer with dual-color immunostaining need to be confirmed with hematoxylin-eosin staining, and minimal criteria for a definitive diagnosis of cancer are still lacking. Copyright © 2011 Elsevier Inc. All rights reserved.

  18. Response Surface Optimization of Rotenone Using Natural Alcohol-Based Deep Eutectic Solvent as Additive in the Extraction Medium Cocktail

    Directory of Open Access Journals (Sweden)

    Zetty Shafiqa Othman

    2017-01-01

    Full Text Available Rotenone is a biopesticide with an amazing effect on aquatic life and insect pests. In Asia, it can be isolated from Derris species roots (Derris elliptica and Derris malaccensis. The previous study revealed the comparable efficiency of alcohol-based deep eutectic solvent (DES in extracting a high yield of rotenone (isoflavonoid to binary ionic liquid solvent system ([BMIM]OTf and organic solvent (acetone. Therefore, this study intends to analyze the optimum parameters (solvent ratio, extraction time, and agitation rate in extracting the highest yield of rotenone extract at a much lower cost and in a more environmental friendly method by using response surface methodology (RSM based on central composite rotatable design (CCRD. By using RSM, linear polynomial equations were obtained for predicting the concentration and yield of rotenone extracted. The verification experiment confirmed the validity of both of the predicted models. The results revealed that the optimum conditions for solvent ratio, extraction time, and agitation rate were 2 : 8 (DES : acetonitrile, 19.34 hours, and 199.32 rpm, respectively. At the optimum condition of the rotenone extraction process using DES binary solvent system, this resulted in a 3.5-fold increase in a rotenone concentration of 0.49 ± 0.07 mg/ml and yield of 0.35 ± 0.06 (%, w/w as compared to the control extract (acetonitrile only. In fact, the rotenone concentration and yield were significantly influenced by binary solvent ratio and extraction time (P<0.05 but not by means of agitation rate. For that reason, the optimal extraction condition using alcohol-based deep eutectic solvent (DES as a green additive in the extraction medium cocktail has increased the potential of enhancing the rotenone concentration and yield extracted.

  19. Online trust building through third party trust transfer and third party protection

    Science.gov (United States)

    Wandoko, Wanda; Saleh Abbas, Bahtiar; Budiastuti, Dyah; Kosala, Raymond

    2017-03-01

    The primary objective of this research is to develop an online trust building mechanism for SME (Small Medium Enterprise). Trust is very important in e-commerce. The nature of online shopping has a greater uncertainty than offline shopping. Seeing as there is an uncertainty that can produce risks, a prospective buyer’s trust is needed. A lot of people’s unwillingness to shop online is caused by their lack of trust toward e-commerce. E-commerce is said to be one of the ways for SME to compete with bigger companies. However, building trust requires immense time and cost. SME with limited resources may experience difficulties in building trust just with their own resources. Base on literature research that needs to be validated in next research, we found that trust can be built through trust transfer from the reputable and well-known trust-mark issuer, and third party protection such as escrow account service and credit card issuer.

  20. Third-party biller compliance guidance emphasizes risk awareness.

    Science.gov (United States)

    Saner, R J

    1999-03-01

    The voluntary compliance guidance for third-party billing companies released by the HHS Office of Inspector General (OIG) in December 1998, like the OIG's previously released guidance directed at hospitals, home health agencies, and clinical laboratories, identifies seven minimum elements for an effective corporate compliance program: written compliance policies, designation of a compliance officer, ongoing training, open lines of communication, guidelines to ensure the enforcement of compliance standards, internal monitoring and auditing of compliance activity, and procedures to respond to and correct errors. Three areas of concern for third-party billing companies are emphasized in the new guidance document: compliance risk, claims documentation, and disclosure of suspected misconduct or fraud.

  1. Political Market Orientation and the Network Party Type

    DEFF Research Database (Denmark)

    Ormrod, Robert P.

    The conceptualisation of a political market orientation (PMO) draws on current marketing thought by focussing on the relationships that exist between the party2 and relevant internal and external stakeholders (Ormrod 2005, 2011a). However, the specific way in which the PMO model is linked...... to the political science literature has until now not been made explicit. The aim of this paper is therefore to demonstrate how Ormrod’s (2005, 2011a) conceptualisation of a PMO can contribute to understanding political science models of party organisation, specifically the ‘Network Party’ developed by Heidar...

  2. An improved model of alliances between political parties

    CERN Document Server

    Bagarello, Fabio

    2016-01-01

    We consider an operatorial model of alliances between three political parties which interact with their electors, with the undecided voters, and with the electors of the other parties. This extends what was done in a previous paper, where this last type of interactions was not considered. Of course, taking them into account makes the system closer to real life. To produce an exactly solvable model, we restrict here to quadratic Hamiltonians, so that the equations of motion turn out to be linear. The dynamics of the so-called {\\em decision functions} are deduced, and some explicit situations are considered in details.

  3. Actively Secure Two-Party Evaluation of Any Quantum Operation

    DEFF Research Database (Denmark)

    Dupuis, Frédéric; Nielsen, Jesper Buus; Salvail, Louis

    2012-01-01

    We provide the first two-party protocol allowing Alice and Bob to evaluate privately even against active adversaries any completely positive, trace-preserving map , given as a quantum circuit, upon their joint quantum input state . Our protocol leaks no more to any active adversary than an ideal ...... functionality for provided Alice and Bob have the cryptographic resources for active secure two-party classical computation. Our protocol is constructed from the protocol for the same task secure against specious adversaries presented in [4]....

  4. Achieving Energy Efficient Ship Operations Under Third Party Management

    DEFF Research Database (Denmark)

    Taudal Poulsen, René; Sornn-Friese, Henrik

    2015-01-01

    of time to produce and provide reliable energy efficiency information cause energy efficiency gaps. The paper brings together the energy efficiency and ship management literatures, demonstrating how ship management models influence energy efficiency in ship operations. Achieving energy efficiency in ship...... operations is particularly challenging under third party ship management. Finally, the paper discusses management implications for shipping companies, which outsource ship management to third parties.......Profitable energy saving measures are often not fully implemented in shipping, causing energy efficiency gaps. The paper identifies energy efficiency gaps in ship operations, and explores their causes. Lack of information on energy efficiency, lack of energy training at sea and onshore and lack...

  5. Three-party quantum secure direct communication against collective noise

    Science.gov (United States)

    He, Ye-Feng; Ma, Wen-Ping

    2017-10-01

    Based on logical quantum states, two three-party quantum secure direct communication protocols are proposed, which can realize the exchange of the secret messages between three parties with the help of the measurement correlation property of six-particle entangled states. These two protocols can be immune to the collective-dephasing noise and the collective-rotation noise, respectively; neither of them has information leakage problem. The one-way transmission mode ensures that they can congenitally resist against the Trojan horse attacks and the teleportation attack. Furthermore, these two protocols are secure against other active attacks because of the use of the decoy state technology.

  6. Secure Two-Party Computation with Low Communication

    DEFF Research Database (Denmark)

    Damgård, Ivan Bjerre; Kölker, Jonas; Faust, Sebastian

    2012-01-01

    We propose a 2-party UC-secure protocol that can compute any function securely. The protocol requires only two messages, communication that is poly-logarithmic in the size of the circuit description of the function, and the workload for one of the parties is also only poly-logarithmic in the size...... of the circuit. This implies, for instance, delegatable computation that requires no expensive off-line phase and remains secure even if the server learns whether the client accepts its results. To achieve this, we define two new notions of extractable hash functions, propose an instantiation based...

  7. Secure Two-Party Computation with Low Communication

    DEFF Research Database (Denmark)

    Damgård, Ivan Bjerre; Faust, Sebastian; Hazay, Carmit

    2011-01-01

    We propose a 2-party UC-secure computation protocol that can compute any function securely. The protocol requires only two messages, communication that is poly-logarithmic in the size of the circuit description of the function, and the workload for one of the parties is also only poly......-logarithmic in the size of the circuit. This implies, for instance, delegatable computation that requires no expensive off-line phase and remains secure even if the server learns whether the client accepts its results. To achieve this, we define a notion of extractable hash functions, propose an instantiation based...

  8. Observing Third-Party Attentional Relationships Affects Infants' Gaze Following: An Eye-Tracking Study

    Science.gov (United States)

    Meng, Xianwei; Uto, Yusuke; Hashiya, Kazuhide

    2017-01-01

    Not only responding to direct social actions toward themselves, infants also pay attention to relevant information from third-party interactions. However, it is unclear whether and how infants recognize the structure of these interactions. The current study aimed to investigate how infants' observation of third-party attentional relationships influence their subsequent gaze following. Nine-month-old, 1-year-old, and 1.5-year-old infants (N = 72, 37 girls) observed video clips in which a female actor gazed at one of two toys after she and her partner either silently faced each other (face-to-face condition) or looked in opposite directions (back-to-back condition). An eye tracker was used to record the infants' looking behavior (e.g., looking time, looking frequency). The analyses revealed that younger infants followed the actor's gaze toward the target object in both conditions, but this was not the case for the 1.5-year-old infants in the back-to-back condition. Furthermore, we found that infants' gaze following could be negatively predicted by their expectation of the partner's response to the actor's head turn (i.e., they shift their gaze toward the partner immediately after they realize that the actor's head will turn). These findings suggested that the sensitivity to the difference in knowledge and attentional states in the second year of human life could be extended to third-party interactions, even without any direct involvement in the situation. Additionally, a spontaneous concern with the epistemic gap between self and other, as well as between others, develops by this age. These processes might be considered part of the fundamental basis for human communication.

  9. Effective implementation of the UNCRPD by Thailand State Party: challenges and potential remedies.

    Science.gov (United States)

    Srisuppaphon, Donruedee; Sriboonroj, Arnon; Riewpaiboon, Wachara; Tangcharoensathien, Viroj

    2017-05-25

    The Thai government ratified the United Nations Convention on the Rights of Persons with Disabilities (UNCRPD) in 2008, and the first progress report by the State Party was issued in 2012. This study assesses and identifies gaps in the Government's implementation of the Convention. Using the Deming Plan-Do-Check-Act Cycle as an analytical framework for continuous quality improvement, we reviewed five documents which are: the 2012 State Party report; the list of issues by the Committee on the Rights of Persons with Disabilities; the 2015 replies to the list of issues by the Thai government; an alternative report produced by Civil Society Organizations (CSOs); and an alternative report produced by the National Human Rights Commission of Thailand. Content analysis is applied to generate the emerging gaps in implementation. Thailand's main advantage is the evolving legal frameworks operating in compliance with the convention, although further amendment is still needed, including effective law enforcement. Conflicting information between the Government's and alternative reports reflects the shortcomings in the information system that intends to support rigorous monitoring and evaluation. Lacking of concrete measures and outcome indicators on certain articles reflects the State Party's limited understanding of the concept of human rights and participatory approaches and insufficient institutional capacities for effective implementation. To rectify these implementation gaps, a few actions are suggested. This includes amending the laws which violate the rights of persons with psychosocial disability; reforming governance where the monitoring bodies are truly independent from implementing agencies; strengthening cross-sectoral actions; and improving information systems which facilitate monitoring and evaluation where Disabled People's Organizations and Civil Society Organizations are recognized as true equal partners. Implementation research can provide evidence for further

  10. Monopolizing, Mutualizing, or Muddling Through: Factions and Party Management in Contemporary Thailand

    Directory of Open Access Journals (Sweden)

    Paul W. Chambers

    2010-01-01

    Full Text Available In democracies throughout the world, intra-party factions manifest themselves in parties and governments. Formal and informal institutions have, however, proved crucial in managing factionalism. This is especially true in Thailand’s emerging parliamentary democracy where the management of factionalism has become a major objective for Thai parties. This study explores factions and factionalism as well as how different types of parties try to manage intra-party dissension especially in the case of Thailand. The findings suggest that management style tends to be a function of a party’s organization, with parties which practice a collegial style tending to be the more successful in controlling intra-party cliques over time. At the same time, the most important tools which party leaderships can use to control factions are the careful use of constitutional provisions and manipulation of party finance.

  11. A party leader is in the system of political communications : theory and practice

    Directory of Open Access Journals (Sweden)

    Nikolai V. Morar

    2016-01-01

    Full Text Available Investigates the place and role of political communication in party leader image-making. The peculiarities of party communication as an important kind of political communication are uncovered. Analyzed types of party communication and gives the characteristic for changes in the context of the common society information. The results of investigation indicate a significant role and importance of party leader in political communications system. One of the most important direction of politics of party leaders are party communication. It is part of a larger concept - political communication. Political communication is a specific type of political relations and is seen as an inseparable element of the political domain of society. The main purpose of the party communication is creating conditions for public policy and public authority through the informational influence of political actors on each other and the social environment. The feature of party communication is that it provides the variety sort of messages and information that circulates within the party between its structural elements and members. Although this information basis on the initiative of the party can be given to the person, citizen, group, to the other party, political actor or political institutions. Understanding the theoretical foundations of party communication, their creative implementation in the development of party circles is the key to the success of the party leader’s activity and authority increasing among the masses.

  12. Improved immunohistochemical evaluation of micrometastases in sentinel lymph nodes of cutaneous melanoma with 'MCW Melanoma Cocktail' – A mixture of monoclonal antibodies to MART-1, melan-A, and tyrosinase

    Directory of Open Access Journals (Sweden)

    Kampalath Bal

    2003-05-01

    Full Text Available Abstract Background MART-1, Melan-A, and Tyrosinase have shown encouraging results for evaluation of melanoma micrometastases in sentinel lymph nodes, as compared to conventionally used S-100 protein and HMB-45. To achieve higher sensitivity, some studies recommend evaluation of three sections, each at intervals of 200 μ. This would mean, routine staining of three adjacent sections in each of the three clusters at intervals of 200 μ, requiring nine slides resulting in added expense. If a cocktail of these antibodies could be used, only one section would be required instead of three generating significant cost savings. Methods We prepared a combination of monoclonal antibodies to these three immunomarkers in optimized dilutions (MART-1, clone M2-7C10, dilution 1:500; Melan-A, clone A103, dilution 1:100; and Tyrosinase, clone T311, dilution 1:50 and designated it as 'MCW melanoma cocktail'. Formalin-fixed paraffin-embedded tissue sections of sentinel lymph nodes from patients with cutaneous melanoma, without macro-metastases were evaluated with this cocktail. Results Melanoma micrometastases were easily detectable with the cocktail in 41 out of 188 slices (8/24 cases. The diagnostic accuracy amongst five pathologists did not show statistically significant difference. Out of 188 slices, 78 had adjacent sections immunostained individually with MART-1 and Melan-A during our previous study. Of these 78 slices, 21 were positive for melanoma micrometastases with MART-1 and Melan-A individually. However, the adjacent section of these slices immunostained with the cocktail detected metastases in four additional slices. Thus, MART-1 and Melan-A could not detect melanoma micrometastases individually in 16% (4/25 of slices positive with the cocktail. Benign capsular nevi were immunoreactive for the cocktail in 4.8% (9/188 slices. All 81 slices of negative test controls (sentinel lymph nodes of mammary carcinoma were interpreted correctly as negative for

  13. A requirement analysis for a multi-party conferencing testbed

    NARCIS (Netherlands)

    Pijpers, S.A.G.

    2014-01-01

    Current videoconferencing services such as Skype and Google+Hangouts provide mechanisms for engaging in multi-party conversations. Although these services provide basic support, they lack functionalities that take into account the users roles and context. Currently, the multimedia research community

  14. Syria: The Evolution of Party and State, 1975-1981.

    Science.gov (United States)

    Olson, Robert W.

    1983-01-01

    In two decades of Ba'thist rule, Syria has evolved into a centralized, one-party, secular, authoritarian nation-state, which may be destabilized in the 1980s as a result of the conflict in Lebanon and internal Islamic opposition. (RM)

  15. 49 CFR 1114.26 - Written interrogatories to parties.

    Science.gov (United States)

    2010-10-01

    .... (d) Limitation under simplified standards. In a case using the Three-Benchmark methodology, each.... (b) Option to produce business records. Where the answer to an interrogatory may be derived or ascertained from the business records of the party upon whom the interrogatory has been served or from an...

  16. Getting Something for Nothing: Trump, Fraud, and the Tea Party

    Science.gov (United States)

    Bordelon, David

    2017-01-01

    David Bordelon identifies himself as one of the majority of Americans who voted to keep Trump out of office. In this article he discusses his confusion by the Tea Party's fixation on welfare fraud, and inability to recognize it in President Trump's behavior. The author says that he can only hope that his confusion will be shared, and progress to…

  17. 39 CFR 955.26 - Representation of the parties.

    Science.gov (United States)

    2010-07-01

    ... 39 Postal Service 1 2010-07-01 2010-07-01 false Representation of the parties. 955.26 Section 955.26 Postal Service UNITED STATES POSTAL SERVICE PROCEDURES RULES OF PRACTICE BEFORE THE POSTAL SERVICE... jurisdiction in which the attorney is licensed to practice law. (b) The term respondent means the U.S. Postal...

  18. Dutch Voters and the Changing Party Space 1989-2006

    NARCIS (Netherlands)

    Aarts, Kees; Thomassen, Jacques J.A.

    2008-01-01

    Elections in the Netherlands in the period 1989-2006 have shown major changes in the supply of political parties and at some points a very large volatility of results. We describe these developments against the background of theories of dealignment and realignment. Specifically, we focus on the

  19. The party political make-up of EU legislative bodies

    NARCIS (Netherlands)

    Warntjen, Andreas; Hix, Simon; Crombez, Christophe

    2008-01-01

    This research note provides an overview of the party political make-up of EU legislative bodies for the period 1979-2004 (Commission: 1979-2007) in terms of the left-right and pro-/anti-Europe dimensions. The various methods of measuring political positions are discussed and compared. Measures for t

  20. 5 CFR 919.950 - Excluded Parties List System

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 2 2010-01-01 2010-01-01 false Excluded Parties List System 919.950 Section 919.950 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT (CONTINUED) CIVIL SERVICE REGULATIONS (CONTINUED) GOVERNMENTWIDE DEBARMENT AND SUSPENSION (NONPROCUREMENT) Definitions § 919.950...

  1. The Power of Opposition Parliamentary Party Groups in European Scrutiny

    NARCIS (Netherlands)

    Holzhacker, Ronald

    2005-01-01

    This paper investigates the power of opposition parliamentary party groups when scrutinising their governments' decision-making concerning European Union issues, an important component in assuring democracy within the emerging multi-level system of governance in the EU. It first sets out the advanta

  2. Party financing and referendum campaigns in EU member states

    NARCIS (Netherlands)

    M. van Klingeren; M. Orozco; J. van Spanje; C. de Vreese

    2015-01-01

    This study has been prepared by the Amsterdam School of Communication Research (ASCoR), part of the Faculty of Social and Behavioural Sciences, University of Amsterdam (UvA). It comprises 1) an up-to-date inventory of party finance rules within the EU Member States, 2) an overview of the regulations

  3. Organizational mediators siding with or against the powerful party?

    NARCIS (Netherlands)

    de Dreu, C.K.W.; Laskewitz, P.; van de Vliert, E.

    1994-01-01

    Examined whether, instead of being impartial, a mediator may side with one party as a function of the disputants' power differences (power balancing theory), the mediator's legitimacy judgments (siding theory), or the disputants' capacity to sanction the mediator (siding theory). A questionnaire stu

  4. The Labour Party's Blunkett Review: A Comprehensive Disappointment

    Science.gov (United States)

    Lichman, Keith

    2014-01-01

    David Blunkett's "Review of Education Structures" for the Labour Party recognises that there is a chaotic and unsatisfactory situation in the English education system but its response is ambiguous and self-contradictory. Its proposals seek to normalise and regulate rather than remedy a system in which lack of democratic accountability,…

  5. Contextualizing Intergroup Contact: Do Political Party Cues Enhance Contact Effects?

    DEFF Research Database (Denmark)

    Sønderskov, Kim Mannemar; Thomsen, Jens Peter Frølund

    2015-01-01

    This article examines intergroup contact effects in different political contexts. We expand on previous efforts of social psychologists by incorporating the messages of political parties as a contextual trigger of group membership awareness in contact situations. We argue that the focus among...

  6. Injunctions against innocent third parties : Case of website blocking

    NARCIS (Netherlands)

    Husovec, Martin

    2013-01-01

    The paper discusses a phenomenon of injunctions against innocent third parties (in a tort law sense). One type of such injunctions, website blocking, is currently appearing in a spotlight around various European jurisdictions as a consequence of the implementation of Art. 8(3) of the Information Soc

  7. 29 CFR 2700.4 - Parties, intervenors, and amici curiae.

    Science.gov (United States)

    2010-07-01

    ..., is a party. In a proceeding instituted by the Secretary under section 105(c)(2) of the Act, 30 U.S.C... miner who has filed a complaint with the Commission under section 105(c)(3) or 111 of the Act, 30 U.S.C... intervention will not unduly delay or prejudice the adjudication of the issues. (ii) Such intervention is not...

  8. Towards automatic addressee identification in multi-party dialogues

    NARCIS (Netherlands)

    Jovanovic, N.; op den Akker, Hendrikus J.A.

    2004-01-01

    The paper is about the issue of addressing in multi-party dialogues. Analysis of addressing behavior in face to face meetings results in the identification of several addressing mechanisms. From these we extract several utterance features and features of non-verbal communicative behavior of a

  9. Limits to Party Autonomy in International Commercial Arbitration

    National Research Council Canada - National Science Library

    Giuditta Cordero-Moss

    2014-01-01

    ... of the legal relationship between the parties, the contract aims at making national law dispensable. If national law is not relevant, and the only basis for regulating the parties’ legal relationship is the contract, it becomes possible and meaningful to standardise contract terms even when contracts are intended to be implemented in a vari...

  10. CPAFFC President Chen Haosu and His Party in Ukraine

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    <正>At the invitation of the Ukraine-China Society (UCS), CPAFFC President Chen Haosu and his party paid a visit to Kiev and Yalta from June 5 to 9. Leonid Kravchuk, honorary president of the UCS and first Ukrainian

  11. Tracing Graduates through Reunion Parties: Secondary Technical Education in Mozambique.

    Science.gov (United States)

    Ziderman, Adrian

    1997-01-01

    A review of enrollments, graduates, and costs for Mozambique secondary technical schools and the results of a follow-up employment survey conducted at graduate reunion parties indicate low internal and external efficiency and high unit costs in the technical education system, particularly "basic" technical schools. Three possible types…

  12. Experiences with the Dutch Working Party on antibiotic policy (SWAB).

    NARCIS (Netherlands)

    Prins, J.M.; Degener, J.E.; Neeling, A.J. de; Gyssens, I.C.J.

    2008-01-01

    The Dutch Working Party on Antibiotic Policy (Stichting Werkgroep AntibioticaBeleid, SWAB) was founded in 1996 as an initiative of the Society for Infectious Diseases, the Dutch Society for Medical Microbiology, and the Dutch Association of Hospital Pharmacists. Its primary goal is to contribute to

  13. Circulation of the Elite in the Chinese Communist Party

    Science.gov (United States)

    2014-09-02

    transformation in China: From ‘ideology-oriented/replacement’ elites to ‘fragmented/ reproductive ’ elites”. Asian Perspective 37: 255–279. Kendall, Maurice...Party membership exceeds 85 mil- lion .” Xinhua, 1 July 2013, retrieved from http://english.cpc.people.com.cn/206972/206974/ 8305636.html. Zang, Xiaowei

  14. Measurement study of multi-party video conferencing

    NARCIS (Netherlands)

    Lu, Y.; Zhao, Y.; Kuipers, F.A.; Van Mieghem, P.

    2010-01-01

    More and more free multi-party video conferencing applications are readily available over the Internet and both Server-to-Client (S/C) or Peer-to-Peer (P2P) technologies are used. Investigating their mechanisms, analyzing their system performance, and measuring their quality are important objectives

  15. SOVIET POLITICAL SCHOOLS, THE COMMUNIST PARTY ADULT INSTRUCTION SYSTEM.

    Science.gov (United States)

    MICKIEWICZ, ELLEN PROPPER

    A STUDY WAS MADE OF SOVIET ADULT POLITICAL EDUCATION MAINLY AS IT APPLIES TO RUSSIAN URBAN AREAS, WHERE THE SYSTEM IS MOST HIGHLY DEVELOPED. THIS SYSTEM, AN AGENCY FOR TRANSMITTING POLITICAL DOCTRINE, FORMS A PART OF THE VAST NETWORK OF FORMAL POLITICAL COMMUNICATION, WHICH INCLUDES THE MASS MEDIA, AGITATION, AND COMMUNIST PARTY LEADERSHIP…

  16. Understanding researchers' openness to external parties influencing academic research

    NARCIS (Netherlands)

    Olmos-Penuela, Julia; Benneworth, Paul; Castro-Martínez, Elena

    2014-01-01

    Ambiguity surrounding the effect of external engagement on academic research has raised questions about what motivates researchers to collaborate with third parties. This paper contributes to this debate by progressing beyond the idea that researchers engage with society simply because of direct ben

  17. Understanding resarchers' openness to external parties influencing academic research

    NARCIS (Netherlands)

    Olmos Peñuela, Julia; Benneworth, Paul; Castro-Martínez, Elena

    2014-01-01

    Ambiguity surrounding the effect of external engagement on academic research has raised questions about what motivates researchers to collaborate with third parties. This paper contributes to this debate by progressing beyond the idea that researchers engage with society simply because of direct ben

  18. Educational Digital Technologies in Developing Countries Challenge Third Party Providers

    Science.gov (United States)

    Passey, Don; Laferrière, Thérèse; Ahmad, Manal Yazbak-Abu; Bhowmik, Miron; Gross, Diana; Price, Janet; Resta, Paul; Shonfeld, Miri

    2016-01-01

    In this conceptual paper, we consider issues and challenges of third party and governmental organisations in planning and implementing access to and uses of digital technologies for learning and teaching in developing countries. We consider failures and weaknesses in the planning and implementation processes highlighted by research in developed…

  19. 41 CFR 105-70.013 - Parties to the hearing.

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Parties to the hearing. 105-70.013 Section 105-70.013 Public Contracts and Property Management Federal Property Management Regulations System (Continued) GENERAL SERVICES ADMINISTRATION Regional Offices-General...

  20. 41 CFR 105-70.017 - Rights of parties.

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Rights of parties. 105-70.017 Section 105-70.017 Public Contracts and Property Management Federal Property Management Regulations System (Continued) GENERAL SERVICES ADMINISTRATION Regional Offices-General...

  1. Secure data structures based on multi-party computation

    DEFF Research Database (Denmark)

    Toft, Tomas

    2011-01-01

    This work considers data structures based on multi-party computation (MPC) primitives: structuring secret (e.g. secret shared and potentially unknown) data such that it can both be queried and updated efficiently. Implementing an oblivious RAM (ORAM) using MPC allows any existing data structure...

  2. Gendering the vote for populist radical-right parties

    NARCIS (Netherlands)

    Spierings, C.H.B.M.; Zaslove, A.S.

    2015-01-01

    Why do more men than women vote for populist radical-right (PRR) parties? And do more men than women still vote for the PRR? Can attitudes regarding gender and gender equality explain these differences (if they exist)? These are the questions that Spierings and Zaslove explore in this article. They

  3. Monitoring multi-party contracts for E-business

    NARCIS (Netherlands)

    Xu, L.

    2004-01-01

    "Monitoring multi-party contracts for E-business" investigates the issues involved in the performance of econtract monitoring of business automations in business to business e-commerce environment. A pro-active monitoring contract model and monitoring mechanism have been designed and developed. A ne

  4. A dynamic digital signature scheme without third parties

    NARCIS (Netherlands)

    Van Elsas, M.; Van der Lubbe, J.C.A.; Weber, J.H.

    2015-01-01

    We propose a digital signature scheme for dynamic coalitions. Particularly, we enable members to join and leave. Our scheme does not need trusted or oblivious third parties (TTPs or OTPs). In this distributed scheme there is a changing secret group key dependent on the members in the group. Each

  5. Star Parties in Mexico extended to Colombia and China

    Science.gov (United States)

    Torres-Peimbert, Silvia; Franco, José

    2016-10-01

    The preparation for The International Year of Astronomy 2009 stirred our interest in preparing star parties in Mexico. The lunar eclipse of February 20th 2008 was the perfect event for the first massive observation in Mexico City that attracted over 25,000 people. To accompany this event there were additional attractions: a massive astronomical lecture, more than 100 telescopes were set up for people to watch the sky, exhibits of astronomical images, children hands-on projects, rock concert, dance performance, and chats with astronomers. Already in 2009 a collective program was organized to involve more than 30 sites in Mexico to hold star parties at the same time once a year. These star parties were more in the spirit of science fairs, that include lectures, astronomy exhibits, children projects, as well as concerts and other cultural displays. The scope of each one of them depended on the local support from volunteers and from the local authorities. After the International Year of Astronomy the group that organized these star parties decided to continue its activities. The main attraction in these fairs has been the opportunity to see the Moon, Jupiter and Saturn (if observable) through a telescope. For this program the presence of the amateur astronomers has been crucial. They have brought their instruments to the sites and have generously taught the public how to look through the telescopes and pointed out to the interesting features on the sky.

  6. The Data Party: Involving Stakeholders in Meaningful Data Analysis

    Science.gov (United States)

    Franz, Nancy K.

    2013-01-01

    A hallmark of Extension includes the involvement of stakeholders in research and program needs assessment, design, implementation, evaluation, and reporting. A data party can be used to enhance this stakeholder involvement specifically in data analysis. This type of event can not only increase client participation in Extension programming and…

  7. Political cynicism, instability and volatility in multi party democracy

    NARCIS (Netherlands)

    Adriaansen, M.L.

    2009-01-01

    Political cynicism has risen in many European democracies in the past decades, while in the same period voter behavior has become less stable. This study investigates the relationship between the two and distinguishes two kinds of voter behavior: hesitation which party to vote for (instability) and

  8. The proscription of parties and the problem of 'miltant democracy'

    DEFF Research Database (Denmark)

    Bourne, Angela

    2011-01-01

    banned. The party bans in Spain highlight weaknesses in leading theoretical accounts of proscription and democratic responses to extremism, weaknesses that are symptomatic of broader problems with the paradigmatic concept of ‘militant democracy’. More specifically, closer examination of the Spanish case...

  9.  "We got the right to party!"

    DEFF Research Database (Denmark)

    Elmeland, Karen; Kolind, Torsten

    2006-01-01

    a very high consumption of alcohol. For instance in 2003 24 % in this age group reported "binge drinking" 3 times or more during the last 30 days. As a consequence of this increased focus, preventive workers together with parents have on the local level (public schools) worked out "Parents......'s right to party....

  10. The Data Party: Involving Stakeholders in Meaningful Data Analysis

    Science.gov (United States)

    Franz, Nancy K.

    2013-01-01

    A hallmark of Extension includes the involvement of stakeholders in research and program needs assessment, design, implementation, evaluation, and reporting. A data party can be used to enhance this stakeholder involvement specifically in data analysis. This type of event can not only increase client participation in Extension programming and…

  11. Sodium butyrate promotes generation of human induced pluripotent stem cells through induction of the miR302/367 cluster.

    Science.gov (United States)

    Zhang, Zhonghui; Wu, Wen-Shu

    2013-08-15

    Small molecules (SM) can greatly enhance the efficiency of induced pluripotent stem (iPS) cell generation, but the mechanisms by which they act have not been fully explored. We show here that an SM cocktail (NaB, PD03259, and SB431542) significantly promotes iPS cell generation from human fibroblasts, and NaB is more potent than the other two common histone deacetylase inhibitors (valproic acid and Trichostatin A) in promoting cellular reprogramming. Our data indicate that the SM cocktail substantially upregulates the miR302/367 cluster expression by increasing the stability and transcriptional level of this microRNA (miRNA) cluster in a manner dependent on the four defined transcription factors (TFs). Among the four TFs, Oct4 in particular appears to be required for the induction of the miR302/367 cluster by the SM cocktail. We also found that NaB alone can enhance the TFs-dependent upregulation of the miR302/367 cluster. Using a promoter reporter assay, we show that the SM cocktail remarkably enhanced the transcriptional activity of the four TFs in the miR302/367 promoter. Notably, attenuation of miRNA302/367 using a miRZip impairs the ability of the SM cocktail in promoting reprogramming. Collectively, these findings suggest that the SM cocktail promotes reprogramming at least partly through the induction of the miR302/367 cluster expression. Further insights into this process may pave the way for the generation of iPS cells using only SM cocktails.

  12. Changing Patterns in the Impact of Information on Party Choice in a Multiparty System

    DEFF Research Database (Denmark)

    Hansen, Kasper Møller

    2009-01-01

    Election Studies. In both multiparty elections, the impact of information has significant consequences for party choice. In 1971, political knowledge seems to be the variable connecting class with party choice, whereas political knowledge seems to be the variable connecting issues with party choice in 2005....... The information effects are greater than the effects observed in similar analyses of the American two-party system, thus suggesting a positive relationship between the number of parties and the impact of political knowledge. Finally, the analysis supports the notion that right-leaning parties tend to fare better...

  13. ISLAMIST PARTY, ELECTORAL POLITICS AND DA’WAH MOBILIZATION AMONG YOUTH: The Prosperous Justice Party (PKS in Indonesia

    Directory of Open Access Journals (Sweden)

    Noorhaidi Hasan

    2012-06-01

    Full Text Available The involvement of the Prosperous Justice Party (PKS, Partai Keadilan Sejahtera in the electoral democratic system in Indonesia raises a question whether it is possible for a political party with a deeply religious ideology to liberalize and entertain democracy. The decision to enter politics does not infrequently entail an abandonment of ideological purity. It represents an acceptance of the political order and power sharing-based politics. While identity and ideology are instrumental in political mobilization for PKS, it has no choice but to negotiate and interact with other political actors through coalitions and parliamentary politics. A modern party involved in a competitive and rule-based system, it is also required to broaden its electorate and reach out to non-Islamist voters. It is intriguing in this context to explore the party’s strategy to maintain its Islamist platforms and the base of support while allying itself with distinctly non-Islamist ruling elites, and engage in the pragmatic ruling coalition. Understanding the paradoxical roles of an Islamist party when involved in the on-going electoral democratic process, this paper also looks at the future of political Islam in Indonesia.

  14. Microbial β-glucosidases from cow rumen metagenome enhance the saccharification of lignocellulose in combination with commercial cellulase cocktail

    Directory of Open Access Journals (Sweden)

    Del Pozo Mercedes V

    2012-09-01

    Full Text Available Abstract Background A complete saccharification of plant polymers is the critical step in the efficient production of bio-alcohols. Beta-glucosidases acting in the degradation of intermediate gluco-oligosaccharides produced by cellulases limit the yield of the final product. Results In the present work, we have identified and then successfully cloned, expressed, purified and characterised 4 highly active beta-glucosidases from fibre-adherent microbial community from the cow rumen. The enzymes were most active at temperatures 45–55°C and pH 4.0-7.0 and exhibited high affinity and activity towards synthetic substrates such as p-nitrophenyl-beta-D-glucopyranoside (pNPbetaG and pNP-beta-cellobiose, as well as to natural cello-oligosaccharides ranging from cellobiose to cellopentaose. The apparent capability of the most active beta-glucosidase, herein named LAB25g2, was tested for its ability to improve, at low dosage (31.25 units g-1 dry biomass, using pNPbetaG as substrate, the hydrolysis of pre-treated corn stover (dry matter content of 20%; 350 g glucan kg-1 dry biomass in combination with a beta-glucosidase-deficient commercial Trichoderma reseei cellulase cocktail (5 units g-1 dry biomass in the basis of pNPbetaG. LAB25g2 increased the final hydrolysis yield by a factor of 20% (44.5 ± 1.7% vs. 34.5 ± 1.5% in control conditions after 96–120 h as compared to control reactions in its absence or in the presence of other commercial beta-glucosidase preparations. The high stability (half-life higher than 5 days at 50°C and pH 5.2 and 2–38000 fold higher (as compared with reported beta-glucosidases activity towards cello-oligosaccharides may account for its performance in supplementation assays. Conclusions The results suggest that beta-glucosidases from yet uncultured bacteria from animal digestomes may be of a potential interest for biotechnological processes related to the effective bio-ethanol production in combination with low dosage of

  15. Political Parties and Popular Representation in Myanmar’s Democratisation Process

    Directory of Open Access Journals (Sweden)

    Kristian Stokke

    2015-01-01

    Full Text Available The article examines the role of political parties in Myanmar’s democratisation process. We argue that the substance of democratisation depends on popular representation through political parties but question their capacity to provide such representation. Examining capacity through the concept of party institutionalisation, we find that most parties have not been able to build effective organisational structures. However, we also find a degree of party institutionalisation in the form of rootedness in society. Political cleavages between those favouring authoritarian rule over democratic rule and Burman nationhood over ethnic notions of nationhood have produced divisions between state-centred parties associated with Myanmar’s authoritarian legacy and society-centred pro-democracy and ethnic parties. Although being less dichotomous than in the past, we argue that these cleavages continue to provide a basis for party identity and rootedness in society. We conclude that further development of political parties and popular representation will be shaped by the relations between parties, the state, and society – where individual parties are shaped according to their tendencies towards state-centred cartel parties or society-centred mass parties.

  16. Assessing the Influence of Political Parties on Public Opinion: The Challenge from Pretreatment Effects

    DEFF Research Database (Denmark)

    Slothuus, Rune

    2016-01-01

    Despite generations of research, political scientists have trouble pinpointing the influence of political parties on public opinion. Recently, scholars have made headway in exploring whether parties in fact shape policy preferences by relying on experimental designs. Yet, the evidence from...

  17. Measuring the effects of social media participation on political party communities

    NARCIS (Netherlands)

    Effing, R.C.; Hillegersberg, van J.; Huibers, T.; Reddick, C.; Aikins, S.K.

    2012-01-01

    Political parties can potentially benefit from Social Media to shape interactions between their members. This chapter presents the Social Media Participation Model (SMPM), which measures the effects of Social Media Participation on political party communities. As people and politicians increasingly

  18. Strong polygamy of quantum correlations in multi-party quantum systems

    Science.gov (United States)

    San Kim, Jeong

    2014-10-01

    We propose a new type of polygamy inequality for multi-party quantum entanglement. We first consider the possible amount of bipartite entanglement distributed between a fixed party and any subset of the rest parties in a multi-party quantum system. By using the summation of these distributed entanglements, we provide an upper bound of the distributed entanglement between a party and the rest in multi-party quantum systems. We then show that this upper bound also plays as a lower bound of the usual polygamy inequality, therefore the strong polygamy of multi-party quantum entanglement. For the case of multi-party pure states, we further show that the strong polygamy of entanglement implies the strong polygamy of quantum discord.

  19. A contextual analysis of party system formation in Nigeria, 1960-2011

    Directory of Open Access Journals (Sweden)

    Aliyu Mukhtar Katsina

    2013-12-01

    Full Text Available This article investigates the parties and party systems in Nigeria since independence. The article argues that the country has witnessed four different types of party systems corresponding to the four democratisation attempts that saw the emergence of constitutional governments since 1960. Special focus is awarded to the transformations of the four party systems and developments in their various institutional designs including regulating the number of parties that participate in the electoral politics of the country. In trying to achieve its objective, the article adopts chronological and thematic approaches. Chronologically, the paper investigates these party systems in successive order beginning with the First Republic when the elements of party politics were first adopted in Nigeria. Thematically, the article focuses on those issues that influenced the formation and transformation of the party systems from the First Republic in 1960 to the present Fourth Republic.

  20. Measuring the effects of social media participation on political party communities

    NARCIS (Netherlands)

    Effing, Robin; van Hillegersberg, Jos; Huibers, T.; Reddick, C.; Aikins, S.K.

    2012-01-01

    Political parties can potentially benefit from Social Media to shape interactions between their members. This chapter presents the Social Media Participation Model (SMPM), which measures the effects of Social Media Participation on political party communities. As people and politicians increasingly