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Sample records for hrm method applied

  1. High Resolution Melting (HRM) applied to wine authenticity.

    Science.gov (United States)

    Pereira, Leonor; Gomes, Sónia; Castro, Cláudia; Eiras-Dias, José Eduardo; Brazão, João; Graça, António; Fernandes, José R; Martins-Lopes, Paula

    2017-02-01

    Wine authenticity methods are in increasing demand mainly in Denomination of Origin designations. The DNA-based methodologies are a reliable means of tracking food/wine varietal composition. The main aim of this work was the study of High Resolution Melting (HRM) application as a screening method for must and wine authenticity. Three sample types (leaf, must and wine) were used to validate the three developed HRM assays (Vv1-705bp; Vv2-375bp; and Vv3-119bp). The Vv1 HRM assay was only successful when applied to leaf and must samples. The Vv2 HRM assay successfully amplified all sample types, allowing genotype discrimination based on melting temperature values. The smallest amplicon, Vv3, produced a coincident melting curve shape in all sample types (leaf and wine) with corresponding genotypes. This study presents sensitive, rapid and efficient HRM assays applied for the first time to wine samples suitable for wine authenticity purposes. Copyright © 2016 Elsevier Ltd. All rights reserved.

  2. Applying high-resolution melting (HRM) technology to identify five commonly used Artemisia species.

    Science.gov (United States)

    Song, Ming; Li, Jingjian; Xiong, Chao; Liu, Hexia; Liang, Junsong

    2016-10-04

    Many members of the genus Artemisia are important for medicinal purposes with multiple pharmacological properties. Often, these herbal plants sold on the markets are in processed forms so it is difficult to authenticate. Routine testing and identification of these herbal materials should be performed to ensure that the raw materials used in pharmaceutical products are suitable for their intended use. In this study, five commonly used Artemisia species included Artemisia argyi, Artemisia annua, Artemisia lavandulaefolia, Artemisia indica, and Artemisia atrovirens were analyzed using high resolution melting (HRM) analysis based on the internal transcribed spacer 2 (ITS2) sequences. The melting profiles of the ITS2 amplicons of the five closely related herbal species are clearly separated so that they can be differentiated by HRM method. The method was further applied to authenticate commercial products in powdered. HRM curves of all the commercial samples tested are similar to the botanical species as labeled. These congeneric medicinal products were also clearly separated using the neighbor-joining (NJ) tree. Therefore, HRM method could provide an efficient and reliable authentication system to distinguish these commonly used Artemisia herbal products on the markets and offer a technical reference for medicines quality control in the drug supply chain.

  3. Employee perceptions of line management performance: applying the AMO theory to explain the effectiveness of line managers' HRM implementation

    NARCIS (Netherlands)

    Bos-Nehles, Anna Christina; van Riemsdijk, Maarten; Looise, Jan C.

    2013-01-01

    Line managers are today seen as increasingly important in effectively implementing HRM practices. Based on the Ability-Motivation-Opportunity (AMO) theory, we predict that line managers' performance in this regard will depend on their ability to apply HRM practices, and that their motivation and the

  4. Advancing multilevel thinking and methods in HRM research

    NARCIS (Netherlands)

    Renkema, Maarten; Meijerink, Jeroen Gerard; Bondarouk, Tatiana

    2016-01-01

    Purpose Despite the growing belief that multilevel research is necessary to advance HRM understanding, there remains a lack of multilevel thinking – the application of principles for multilevel theory building. The purpose of this paper is to propose a systematic approach for multilevel HRM

  5. High-resolution melting (HRM) analysis as a feasible method for detecting spinal muscular atrophy via dried blood spots.

    Science.gov (United States)

    Er, Tze-Kiong; Kan, Tzu-Min; Su, Yu-Fa; Liu, Ta-Chih; Chang, Jan-Gowth; Hung, Shih-Ya; Jong, Yuh-Jyh

    2012-11-12

    Spinal muscular atrophy (SMA) is a neurodegenerative disease with the leading genetic cause of infant mortality. More than 95% of patients with SMA have a homozygous disruption in the survival motor neuron1 (SMN1) gene, caused by mutation, deletion, or rearrangement. Recently, treatment in humans in the immediate postnatal period, prior to the development of weakness or very early in the course of the disease, may be effective. Therefore, our objective was to establish a feasible method for SMA screening. High-resolution melting (HRM) analysis is rapidly becoming the most important mutation-scanning methodology that allows mutation scanning and genotyping without the need for costly labeled oligonucleotides. In the current study, we aim to develop a method for identifying the substitution of single nucleotide in SMN1 exon 7 (c.840C>T) by HRM analysis. Genomic DNA was extracted from peripheral blood samples and dried blood spots obtained from 30 patients with SMA and 30 normal individuals. All results were previously confirmed by denaturing high-performance liquid chromatography (DHPLC). In order to identify the substitution of single nucleotide in SMN1 exon 7 (c.840C>T) by HRM analysis, a primer set was used in HRM analysis. At first, we failed to identify the substitution of single nucleotide in SMN1 exon 7 (c.840C>T) by HRM analysis because the homozygous CC and homozygous TT cannot be distinguished by HRM analysis. Therefore, all samples were mixed with a known SMN1/SMN2 copy number (SMN1/SMN2=0:3), which we may call driver. This strategy is used to differentiate between homozygous CC and homozygous TT. After mixing with driver, the melting profile of homozygous CC becomes heteroduplex; however, the homozygous TT remains the same in the normalized and temperature-shifted difference plots. HRM analysis can be successfully applied to screen SMA via DNA obtained from whole blood and dried blood spots. We strongly believe that HRM analysis, a high-throughput method

  6. Applying high-resolution melting (HRM) technology to olive oil and wine authenticity.

    Science.gov (United States)

    Pereira, Leonor; Gomes, Sónia; Barrias, Sara; Fernandes, José Ramiro; Martins-Lopes, Paula

    2018-01-01

    Olive oil and wine production have a worldwide economic impact. Their market reliability is under great concern because of the increasing number of fraud and adulteration attempts. The need for a traceability system in all its extension is crucial particularly for the cases of olive oils and wines with certified labels, in which only a limited number of olives and grapevine varieties, respectively, are allowed in a restricted well-defined geographical area. Molecular markers have been vastly applied to the food sector, and in particular High-Resolution DNA Melting technology has been successfully applied for olive oil and wine authentication, as part of the traceability system. In this review, the applications of HRM and their usefulness for this sector considering, Safety, Security and Authenticity will be reviewed. A broad overview of the HRM technique will be presented, focusing on the aspects that are crucial for its success, in particular the new generation of fluorescent dsDNA dyes used for amplicon detection and quantification, and the data analysis. A brief outlook on the olive oil and wine authenticity procedures, based on new DNA technology advances, and in which way this may influence the future establishment of a traceability system will be discussed. Copyright © 2017 Elsevier Ltd. All rights reserved.

  7. Development of a rapid HRM genotyping method for detection of dog-derived Giardia lamblia.

    Science.gov (United States)

    Tan, Liping; Yu, Xingang; Abdullahi, Auwalu Yusuf; Wu, Sheng; Zheng, Guochao; Hu, Wei; Song, Meiran; Wang, Zhen; Jiang, Biao; Li, Guoqing

    2015-11-01

    Giardia lamblia is a zoonotic flagellate protozoan in the intestine of human and many mammals including dogs. To assess a threat of dog-derived G. lamblia to humans, the common dog-derived G. lamblia assemblages A, C, and D were genotyped by high-resolution melting (HRM) technology. According to β-giardin gene sequence, the qPCR-HRM primers BG5 and BG7 were designed. A series of experiments on the stability, sensitivity, and accuracy of the HRM method were also tested. Results showed that the primers BG5 and BG7 could distinguish among three assemblages A, C, and D, which Tm value differences were about 1 °C to each other. The melting curves of intra-assay reproducibility were almost coincided, and those of inter-assay reproducibility were much the same shape. The lowest detection concentration was about 5 × 10(-6)-ng/μL sample. The genotyping results from 21 G. lamblia samples by the HRM method were in complete accordance with sequencing results. It is concluded that the HRM genotyping method is rapid, stable, specific, highly sensitive, and suitable for clinical detection and molecular epidemiological survey of dog-derived G. lamblia.

  8. Developing Critical Understanding in HRM Students: Using Innovative Teaching Methods to Encourage Deep Approaches to Study

    Science.gov (United States)

    Butler, Michael J. R.; Reddy, Peter

    2010-01-01

    Purpose: This paper aims to focus on developing critical understanding in human resource management (HRM) students in Aston Business School, UK. The paper reveals that innovative teaching methods encourage deep approaches to study, an indicator of students reaching their own understanding of material and ideas. This improves student employability…

  9. HRM and SNaPshot as alternative forensic SNP genotyping methods.

    Science.gov (United States)

    Mehta, Bhavik; Daniel, Runa; McNevin, Dennis

    2017-09-01

    Single nucleotide polymorphisms (SNPs) have been widely used in forensics for prediction of identity, biogeographical ancestry (BGA) and externally visible characteristics (EVCs). Single base extension (SBE) assays, most notably SNaPshot® (Thermo Fisher Scientific), are commonly used for forensic SNP genotyping as they can be employed on standard instrumentation in forensic laboratories (e.g. capillary electrophoresis). High resolution melt (HRM) analysis is an alternative method and is a simple, fast, single tube assay for low throughput SNP typing. This study compares HRM and SNaPshot®. HRM produced reproducible and concordant genotypes at 500 pg, however, difficulties were encountered when genotyping SNPs with high GC content in flanking regions and differentiating variants of symmetrical SNPs. SNaPshot® was reproducible at 100 pg and is less dependent on SNP choice. HRM has a shorter processing time in comparison to SNaPshot®, avoids post PCR contamination risk and has potential as a screening tool for many forensic applications.

  10. hrm 595,uop hrm 595,uop hrm 595 complete course,uop hrm 595 entire course

    OpenAIRE

    admin

    2018-01-01

    UOP HRM 595 All Assignments NEW Check this A+ tutorial guideline at http://www.assignmentcloud.com/hrm-595-uop/hrm-595-all-assignments-new For more classes visit http://www.assignmentcloud.com HRM 595 All Assignments NEW HRM 595 Week 1 Talent Acquisition, Management, & Retention Strategies NEW HRM 595 Week 2 Labor Relations Guide for Management NEW HRM 595 Week 3 Career Development and Succession Planning NEW HRM 595 Week 4 Compensation, Benefits, ...

  11. Assessment for Melting Temperature Measurement of Nucleic Acid by HRM.

    Science.gov (United States)

    Wang, Jing; Pan, Xiaoming; Liang, Xingguo

    2016-01-01

    High resolution melting (HRM), with a high sensitivity to distinguish the nucleic acid species with small variations, has been widely applied in the mutation scanning, methylation analysis, and genotyping. For the aim of extending HRM for the evaluation of thermal stability of nucleic acid secondary structures on sequence dependence, we investigated effects of the dye of EvaGreen, metal ions, and impurities (such as dNTPs) on melting temperature ( T m ) measurement by HRM. The accuracy of HRM was assessed as compared with UV melting method, and little difference between the two methods was found when the DNA T m was higher than 40°C. Both insufficiency and excessiveness of EvaGreen were found to give rise to a little bit higher T m , showing that the proportion of dye should be considered for precise T m measurement of nucleic acids. Finally, HRM method was also successfully used to measure T m s of DNA triplex, hairpin, and RNA duplex. In conclusion, HRM can be applied in the evaluation of thermal stability of nucleic acid (DNA or RNA) or secondary structural elements (even when dNTPs are present).

  12. Negative Impact of HRM Complementarity on Knowledge Transfer in MNCs

    DEFF Research Database (Denmark)

    Minbaeva, Dana

    2005-01-01

    This paper explores reasons for negative complementarity among HRM practices. It isbuilt upon the premise that there are certain HRM practices influencing extrinsic andintrinsic motivation of knowledge receivers. If those HRM practices are applied in acomplementary way, their impact on knowledge...

  13. HRM Model in Tourism, Based on Dialectical Systems Theory

    Directory of Open Access Journals (Sweden)

    Simona Šarotar Žižek

    2015-12-01

    Full Text Available A human resources management (HRM model integrating trends in HRM with trends in tourism into a dialectical system by the Dialectical Systems Theory (DST. HRM strategy, integrated within the tourism organization’s (to’s strategy is implemented through functional strategies helping their users to achieve a requisitely holistic (rh HRM strategy replacing the prevailing one-sided ones. to’s strategy covers: employees (1 planning, (2 acquisition and selection, (3 development and training, (4 diversity management, (5 teamwork and creativity, (6 motivation and rewarding, (7 stress reduction and health, (8 relationships, (9 personal holism, (10 well-being, (11 work and results assessment; etc. Everyone matters; their synergy is crucial. An innovated HRM model for TOS, which applies employees’, organizations’ rh and integrates new knowledge about HRM. HRM belongs to central managers’ tools. Their HRM must be adapted for TOS, where employees are crucial.

  14. Does e-HRM lead to better HRM service?

    NARCIS (Netherlands)

    Bondarouk, Tanya; Harms, Rainer; Lepak, David

    2017-01-01

    Electronic Human Resource Management (e-HRM) is often assumed to increase HRM service quality. This paper empirically examines the relationship between e-HRM and HRM service quality and addresses two calls from recent e-HRM studies, namely to highlight (i) the importance of the interplay between

  15. The Impact of Narcoterrorism on HRM Systems

    DEFF Research Database (Denmark)

    Ramirez, Jacobo; Madero, Sergio; Muñiz, Carlos

    2016-01-01

    in narcoterrorism environments. Identifying the similarities of objects and issues in the narcoterrorism context can help develop an understanding of this local phenomenon, with implications for the HRM and terrorism literatures on a global scale. Our findings highlight the importance of reinforcing values......This paper presents a study on the emergence of human resource management (HRM) systems in a narcoterrorism context based on a mixed-method approach. We conducted 40 interviews at 24 firms in Mexico to explore emerging HRM systems in narcoterrorism environments. The interviews were complemented...... with a quantitative content analysis of 204 news stories to explore the direct and indirect impacts of narcoterrorism on firms. Our analysis of firms’ responses with respect to HRM systems, which is supported by our quantitative analysis on the impacts of narcoterrorism, indicates that trust appears to shape HRM...

  16. [GSTP1, APC and RASSF1 gene methylation in prostate cancer samples: comparative analysis of MS-HRM method and Infinium HumanMethylation450 BeadChip beadchiparray diagnostic value].

    Science.gov (United States)

    Skorodumova, L O; Babalyan, K A; Sultanov, R; Vasiliev, A O; Govorov, A V; Pushkar, D Y; Prilepskaya, E A; Danilenko, S A; Generozov, E V; Larin, A K; Kostryukova, E S; Sharova, E I

    2016-11-01

    There is a clear need in molecular markers for prostate cancer (PC) risk stratification. Alteration of DNA methylation is one of processes that occur during ÐÑ progression. Methylation-sensitive PCR with high resolution melting curve analysis (MS-HRM) can be used for gene methylation analysis in routine laboratory practice. This method requires very small amounts of DNA for analysis. Numerous results have been accumulated on DNA methylation in PC samples analyzed by the Infinium HumanMethylation450 BeadChip (HM450). However, the consistency of MS-HRM results with chip hybridization results has not been examined yet. The aim of this study was to assess the consistency of results of GSTP1, APC and RASSF1 gene methylation analysis in ÐÑ biopsy samples obtained by MS-HRM and chip hybridization. The methylation levels of each gene determined by MS-HRM were statistically different in the group of PC tissue samples and the samples without signs of tumor growth. Chip hybridization data analysis confirmed the results obtained with the MS-HRM. Differences in methylation levels between tumor tissue and histologically intact tissue of each sample determined by MS-HRM and chip hybridization, were consistent with each other. Thus, we showed that the assessment of GSTP1, APC and RASSF1 gene methylation analysis using MS-HRM is suitable for the design of laboratory assays that will differentiate the PC tissue from the tissue without signs of tumor growth.

  17. Future-concepts of HRM in perspective

    DEFF Research Database (Denmark)

    Neisig, Margit

    This article deals with a systemic perspective on the transition of HRM. The field of study adresses how four recent contributions on HRM transition might be understood according to the framework of the reflective business paradigm (Holmström, 2005). The contributions are building on different...... streams of applied research penetrating future-concepts. Each of the contributions is from highly influential knowledge institutions. The contributions are chosen as to illuminate different elements of the HRM transition. A Luhmann based approach is applied creating a meta-analysis (a third order analysis......) of how the four contributions on HRM transition might be understood according to the framework of the reflective society and business paradigm...

  18. HRM Cd-ROM

    NARCIS (Netherlands)

    drs. C.A.G. Huijsmans; ir. R.B. Opsteeg; drs. H.J.J.L. Seegers

    2006-01-01

    De cd-rom HRM biedt een compleet en actueel overzicht van het totale HRM-gebied. Het bevat een unieke verzameling informatie over personeelsmanagement en sociaal beleid. Deze cd-rom geeft richtlijnen, maatregelen en handige tips op het gebied van personeelsmanagement. Het is samen met het zakboek de

  19. Study of Commercially Available Lobelia chinensis Products Using Bar-HRM Technology.

    Science.gov (United States)

    Sun, Wei; Yan, Song; Li, Jingjian; Xiong, Chao; Shi, Yuhua; Wu, Lan; Xiang, Li; Deng, Bo; Ma, Wei; Chen, Shilin

    2017-01-01

    There is an unmet need for herbal medicine identification using a fast, sensitive, and easy-to-use method that does not require complex infrastructure and well-trained technicians. For instance, the detection of adulterants in Lobelia chinensis herbal product has been challenging, since current detection technologies are not effective due to their own limits. High Resolution Melting (HRM) has emerged as a powerful new technology for clinical diagnosis, research in the food industry and in plant molecular biology, and this method has already highlighted the complexity of species identification. In this study, we developed a method of species specific detection of L. chinensis using HRM analysis combined with internal transcribed spacer 2. We then applied this method to commercial products purporting to contain L . chinensis . Our results demonstrated that HRM can differentiate L. chinensis from six common adulterants. HRM was proven to be a fast and accurate technique for testing the authenticity of L. chinensis in herbal products. Based on these results, a HRM approach for herbal authentication is provided.

  20. High resolution melting analysis (HRM) for the assessment of clonality in feline B-cell lymphomas.

    Science.gov (United States)

    Henrich, Manfred; Scheffold, Svenja; Hecht, Werner; Reinacher, Manfred

    2018-06-01

    Analysis of clonality is gaining importance in diagnosing lymphomas in veterinary medicine. Usually, PCR for the analysis of antigen receptor rearrangement (PARR) is followed by electrophoretic separation of the PCR products. Aim of this study was to test the feasibility of HRM for the assessment of clonality in B-cell lymphomas of cats. High resolution melting analysis differentiates PCR products by their different melting point using the decrease in fluorescence of an intercalating dye during melting of the PCR product. Additionally, the method is easy to use with no post-PCR manipulation of the samples. Forty-seven feline B-cell lymphomas and 31 reactive lymphatic proliferations of cats were investigated by PARR followed either by capillary electrophoresis or an HRM assay. To objectify the interpretation of the HRM results a recently published mathematical approach was applied to the melting curve. To overcome discrepancies between the visual interpretation and the mathematical approach, the latter was modified to include testing of reproducibility and recognition of pseudoclonality. In 11 of 47 lymphoma cases clonal populations were detectable by HRM assay compared to 14 of 47 lymphomas in which clonal populations were detected by capillary electrophoresis assay. Neither of the methods showed a clonal pattern in any of the reactive samples. However, the HRM assay showed a unique pattern in cases of follicular lymphatic hyperplasia that had no corresponding pattern in capillary electrophoresis. The capillary electrophoresis assay could identify 3 lymphomas that were not detected by the HRM assay and is therefore regarded superior to the HRM assay. The comparison however, was hampered by the overall bad performance of the PARR, that might be the consequence of insufficient primer binding due to somatic hypermutation of the binding sites during antigen stimulated proliferation of the B lymphocytes. Copyright © 2018 Elsevier B.V. All rights reserved.

  1. HRM OPTIMIZATION FOR ECHOBIKE

    OpenAIRE

    Pan, Shiyuan

    2014-01-01

    The purpose of this thesis was to help a Chinese manufacturing factory Echobike to in crease its current productivity and offer guidance for its future expansion. Through the study of Chinese HRM in SMEs, it provided a general overview of advantages and dif ficulties of external economy condition and could be seen as the preparation for Echobike to implement a HRM system. On the other hand, the results of the qualitative research interview with its employees revealed the shortness of Echobike...

  2. A method to couple HEM and HRM two-phase flow models

    Energy Technology Data Exchange (ETDEWEB)

    Herard, J.M.; Hurisse, O. [Elect France, Div Rech and Dev, Dept Mecan Fluides Energies and Environm, F-78401 Chatou (France); Hurisse, O. [Univ Aix Marseille 1, Ctr Math and Informat, Lab Anal Topol and Probabil, CNRS, UMR 6632, F-13453 Marseille 13 (France); Ambroso, A. [CEA Saclay, DEN, DM2S, SFME, LETR, 91 - Gif sur Yvette (France)

    2009-04-15

    We present a method for the unsteady coupling of two distinct two-phase flow models (namely the Homogeneous Relaxation Model, and the Homogeneous Equilibrium Model) through a thin interface. The basic approach relies on recent works devoted to the interfacial coupling of CFD models, and thus requires to introduce an interface model. Many numerical test cases enable to investigate the stability of the coupling method. (authors)

  3. A method to couple HEM and HRM two-phase flow models

    International Nuclear Information System (INIS)

    Herard, J.M.; Hurisse, O.; Hurisse, O.; Ambroso, A.

    2009-01-01

    We present a method for the unsteady coupling of two distinct two-phase flow models (namely the Homogeneous Relaxation Model, and the Homogeneous Equilibrium Model) through a thin interface. The basic approach relies on recent works devoted to the interfacial coupling of CFD models, and thus requires to introduce an interface model. Many numerical test cases enable to investigate the stability of the coupling method. (authors)

  4. The strategic value of e-HRM: results from an exploratory study in a governmental organization

    NARCIS (Netherlands)

    Bondarouk, Tatiana; Ruel, Hubertus Johannes Maria

    2013-01-01

    This paper presents results from an exploratory study in a governmental organization on the strategic value of electronic human resource management (e-HRM). By applying the organizational capabilities approach, and by means of mixed research methods, data were collected on two generally acclaimed

  5. Diversity Management and Its Impact on HRM Practices: Evidence from Kuwaiti Companies

    Directory of Open Access Journals (Sweden)

    Oualid ABID

    2017-11-01

    Full Text Available The purpose of this paper is to analyze the Diversity Management implementation in Kuwaiti context and try to reveal its effects on human resource management practices. Data was collected through questionnaires from a variety of companies in the service sector in Kuwait. Convenient sampling method is applied. Among the questionnaires distributed 354 useful ones from 87 different companies were considered. An exploratory factor analysis (EFA with varimax rotation is used to determine the underlying dimensions of HRM and DM. Furthermore, a regression analysis is used to measure the effects of diversity management on HRM. The major findings of this study are as follows: first, there is a positive significant relationship between Diversity Management policies and Human Resources Management practices. The “ability to manage people from different backgrounds” have the most significant effect on HRM practices, followed by “Ability to work in harmony with people from different cultures” component, then “Avoidance of discrimination” component.

  6. Applied Bayesian hierarchical methods

    National Research Council Canada - National Science Library

    Congdon, P

    2010-01-01

    ... . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2 Posterior Inference from Bayes Formula . . . . . . . . . . . . 1.3 Markov Chain Monte Carlo Sampling in Relation to Monte Carlo Methods: Obtaining Posterior...

  7. Methods of applied mathematics

    CERN Document Server

    Hildebrand, Francis B

    1992-01-01

    This invaluable book offers engineers and physicists working knowledge of a number of mathematical facts and techniques not commonly treated in courses in advanced calculus, but nevertheless extremely useful when applied to typical problems in many different fields. It deals principally with linear algebraic equations, quadratic and Hermitian forms, operations with vectors and matrices, the calculus of variations, and the formulations and theory of linear integral equations. Annotated problems and exercises accompany each chapter.

  8. UOP HRM 531 Entire Course NEW

    OpenAIRE

    ADMIN

    2018-01-01

    UOP HRM 531 Entire Course NEW Check this A+ tutorial guideline at http://www.hrm531assignment.com/hrm-531-uop/hrm-531-entire-course -latest For more classes visit http://www.hrm531assignment.com HRM 531 Week 1 Employment Law Report (2 Papers) HRM 531 Week 2 Change Management Impact (2 Papers) HRM 531 Week 3 Individual Compensation and Benefits Strategy (2 Papers) HRM 531 Week 3 Team Salary Inequities Case Study AstraZeneca (2 Papers) HRM 531 W...

  9. A new method for simultaneous detection and discrimination of Bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) using real time PCR with high resolution melting (HRM) analysis.

    Science.gov (United States)

    Marin, M S; Quintana, S; Leunda, M R; Recavarren, M; Pagnuco, I; Späth, E; Pérez, S; Odeón, A

    2016-01-01

    Bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) are antigenically and genetically similar. The aim of this study was to develop a simple and reliable one-step real time PCR assay with high resolution melting (HRM) analysis for the simultaneous detection and differentiation of BoHV-1 and BoHV-5. Optimization of assay conditions was performed with DNA from reference strains. Then, DNA from field isolates, clinical samples and tissue samples of experimentally infected animals were studied by real time PCR-HRM. An efficient amplification of real time PCR products was obtained, and a clear melting curve and appropriate melting peaks for both viruses were achieved in the HRM curve analysis for BoHV type identification. BoHV was identified in all of the isolates and clinical samples, and BoHV types were properly differentiated. Furthermore, viral DNA was detected in 12/18 and 7/18 samples from BoHV-1- and BoHV-5-infected calves, respectively. Real time PCR-HRM achieved a higher sensitivity compared with virus isolation or conventional PCR. In this study, HRM was used as a novel procedure. This method provides rapid, sensitive, specific and simultaneous detection of bovine alpha-herpesviruses DNA. Thus, this technique is an excellent tool for diagnosis, research and epidemiological studies of these viruses in cattle. Copyright © 2015 Elsevier B.V. All rights reserved.

  10. HRM-Earth.dk

    DEFF Research Database (Denmark)

    Hjalager, Anne-Mette; Holt Larsen, Henrik; Znaider, Ruth

    Undersøgelsen tager temperaturen på Human Resource Management i Danmark. Der ses på brugen af HRM-redskaber og virkemidler, som generelt bliver bredere. HRM bliver i stigende grad et strategisk indsatsområde for virksomhederne. Undersøgelsen kortlægger endvidere faktorer omkring outsourcing af HR......-funktionen, incitamenter og personalegoder, etik, performance samt kønsproblematikker i HR-arbejdet. Cranet er en internationalt koordineret analyse, som afvikles parallelt i 40 lande....

  11. HRM - what is next?

    DEFF Research Database (Denmark)

    Neisig, Margit

    2015-01-01

    New managerialism is challenged as higher ordered reflection is needed to cope with com-plexity and uncertainty. A literature gap exists in how HRM and NPM co-evolves from new managerialism towards new governance, and how to cope with distrust in the highly polycentric and multilayered transition...... and aspects of what is needed in addressing distrust as part of this change process. The analysis combines three sub-analyzes synthesized in this paper: • a meta-analysis of four contributions on HRM future-concepts • a comparison of the evolvement of performance management mo¬dels of four Danish muni...

  12. A refined, rapid and reproducible high resolution melt (HRM-based method suitable for quantification of global LINE-1 repetitive element methylation

    Directory of Open Access Journals (Sweden)

    Tse M Yat

    2011-12-01

    Full Text Available Abstract Background The methylation of DNA is recognized as a key mechanism in the regulation of genomic stability and evidence for its role in the development of cancer is accumulating. LINE-1 methylation status represents a surrogate measure of genome-wide methylation. Findings Using high resolution melt (HRM curve analysis technology, we have established an in-tube assay that is linear (r > 0.9986 with a high amplification efficiency (90-105%, capable of discriminating between partcipant samples with small differences in methylation, and suitable for quantifying a wide range of LINE-1 methylation levels (0-100%--including the biologically relevant range of 50-90% expected in human DNA. We have optimized this procedure to perform using 2 μg of starting DNA and 2 ng of bisulfite-converted DNA for each PCR reaction. Intra- and inter-assay coefficients of variation were 1.44% and 0.49%, respectively, supporting the high reproducibility and precision of this approach. Conclusions In summary, this is a completely linear, quantitative HRM PCR method developed for the measurement of LINE-1 methylation. This cost-efficient, refined and reproducible assay can be performed using minimal amounts of starting DNA. These features make our assay suitable for high throughput analysis of multiple samples from large population-based studies.

  13. Rapid detection of Echinococcus species by a high-resolution melting (HRM) approach.

    Science.gov (United States)

    Santos, Guilherme Brzoskowski; Espínola, Sergio Martín; Ferreira, Henrique Bunselmeyer; Margis, Rogerio; Zaha, Arnaldo

    2013-11-14

    High-resolution melting (HRM) provides a low-cost, fast and sensitive scanning method that allows the detection of DNA sequence variations in a single step, which makes it appropriate for application in parasite identification and genotyping. The aim of this work was to implement an HRM-PCR assay targeting part of the mitochondrial cox1 gene to achieve an accurate and fast method for Echinococcus spp. differentiation. For melting analysis, a total of 107 samples from seven species were used in this study. The species analyzed included Echinococcus granulosus (n = 41) and Echinococcus ortleppi (n = 50) from bovine, Echinococcus vogeli (n = 2) from paca, Echinococcus oligarthra (n = 3) from agouti, Echinococcus multilocularis (n = 6) from monkey and Echinococcus canadensis (n = 2) and Taenia hydatigena (n = 3) from pig. DNA extraction was performed, and a 444-bp fragment of the cox1 gene was amplified. Two approaches were used, one based on HRM analysis, and a second using SYBR Green Tm-based. In the HRM analysis, a specific profile for each species was observed. Although some species exhibited almost the same melting temperature (Tm) value, the HRM profiles could be clearly discriminated. The SYBR Green Tm-based analysis showed differences between E. granulosus and E. ortleppi and between E. vogeli and E. oligarthra. In this work, we report the implementation of HRM analysis to differentiate species of the genus Echinococcus using part of the mitochondrial gene cox1. This method may be also potentially applied to identify other species belonging to the Taeniidae family.

  14. Analysis of HIV using a high resolution melting (HRM) diversity assay: automation of HRM data analysis enhances the utility of the assay for analysis of HIV incidence.

    Science.gov (United States)

    Cousins, Matthew M; Swan, David; Magaret, Craig A; Hoover, Donald R; Eshleman, Susan H

    2012-01-01

    HIV diversity may be a useful biomarker for discriminating between recent and non-recent HIV infection. The high resolution melting (HRM) diversity assay was developed to quantify HIV diversity in viral populations without sequencing. In this assay, HIV diversity is expressed as a single numeric HRM score that represents the width of a melting peak. HRM scores are highly associated with diversity measures obtained with next generation sequencing. In this report, a software package, the HRM Diversity Assay Analysis Tool (DivMelt), was developed to automate calculation of HRM scores from melting curve data. DivMelt uses computational algorithms to calculate HRM scores by identifying the start (T1) and end (T2) melting temperatures for a DNA sample and subtracting them (T2 - T1 =  HRM score). DivMelt contains many user-supplied analysis parameters to allow analyses to be tailored to different contexts. DivMelt analysis options were optimized to discriminate between recent and non-recent HIV infection and to maximize HRM score reproducibility. HRM scores calculated using DivMelt were compared to HRM scores obtained using a manual method that is based on visual inspection of DNA melting curves. HRM scores generated with DivMelt agreed with manually generated HRM scores obtained from the same DNA melting data. Optimal parameters for discriminating between recent and non-recent HIV infection were identified. DivMelt provided greater discrimination between recent and non-recent HIV infection than the manual method. DivMelt provides a rapid, accurate method of determining HRM scores from melting curve data, facilitating use of the HRM diversity assay for large-scale studies.

  15. CURRENT TRENDS IN HRM

    OpenAIRE

    Otilia ALBU; Lucia MOROŞAN-DĂNILĂ

    2009-01-01

    The goal of this article is to establish the importance of human resource management (HRM) and how it emerged, to provide some evidence of its context, to discuss its potential and future development. Many specialists underlined the fact that human resource requires more attention and careful management then any other resource of an organization. The role of the HR manager must parallel the needs of the changing organization. Successful organizations are becoming more adaptable, resilient, qu...

  16. HRM Implementation by Multiple HRM Actors : A Social Exchange Perspective

    NARCIS (Netherlands)

    Bos-Nehles, Anna Christina; Meijerink, Jeroen Gerard

    2018-01-01

    In this study, we understand HRM implementation as a social process that depends on the social exchange relationships between line managers and both HR professionals and employees. As such, we offer a fresh approach to understanding HRM implementation by concentrating on the social exchange among

  17. Can HRM make a difference?

    DEFF Research Database (Denmark)

    Bramming, Pia

    2004-01-01

    This article is about how a constructivist observation of development within Human Resource Management (HRM) opens the possibility for communicating about development in the language of possibility, seen in contrast to a language of deficiency. HRM is discussed as a paradoxical development concep...

  18. HRM i Danmark efter krisen

    DEFF Research Database (Denmark)

    Bévort, Frans; Holt Larsen, Henrik; Hjalager, Anne-Mette

    Kortlægning af praksis for Human Resource Management (HRM) i alle organisationer i Danmark med mere end 100 ansatte.......Kortlægning af praksis for Human Resource Management (HRM) i alle organisationer i Danmark med mere end 100 ansatte....

  19. High resolution melting (HRM) analysis of DNA--its role and potential in food analysis.

    Science.gov (United States)

    Druml, Barbara; Cichna-Markl, Margit

    2014-09-01

    DNA based methods play an increasing role in food safety control and food adulteration detection. Recent papers show that high resolution melting (HRM) analysis is an interesting approach. It involves amplification of the target of interest in the presence of a saturation dye by the polymerase chain reaction (PCR) and subsequent melting of the amplicons by gradually increasing the temperature. Since the melting profile depends on the GC content, length, sequence and strand complementarity of the product, HRM analysis is highly suitable for the detection of single-base variants and small insertions or deletions. The review gives an introduction into HRM analysis, covers important aspects in the development of an HRM analysis method and describes how HRM data are analysed and interpreted. Then we discuss the potential of HRM analysis based methods in food analysis, i.e. for the identification of closely related species and cultivars and the identification of pathogenic microorganisms. Copyright © 2014 Elsevier Ltd. All rights reserved.

  20. The Relationship between the Level and Modality of HRM Metrics, Quality of HRM Practice and Organizational Performance

    OpenAIRE

    Nina Pološki Vokić

    2011-01-01

    The paper explores the relationship between the way organizations measure HRM and overall quality of HRM activities, as well as the relationship between HRM metrics used and financial performance of an organization. In the theoretical part of the paper modalities of HRM metrics are grouped into five groups (evaluating HRM using accounting principles, evaluating HRM using management techniques, evaluating individual HRM activities, aggregate evaluation of HRM, and evaluating HRM de...

  1. Methylation-Sensitive High Resolution Melting (MS-HRM).

    Science.gov (United States)

    Hussmann, Dianna; Hansen, Lise Lotte

    2018-01-01

    Methylation-Sensitive High Resolution Melting (MS-HRM) is an in-tube, PCR-based method to detect methylation levels at specific loci of interest. A unique primer design facilitates a high sensitivity of the assays enabling detection of down to 0.1-1% methylated alleles in an unmethylated background.Primers for MS-HRM assays are designed to be complementary to the methylated allele, and a specific annealing temperature enables these primers to anneal both to the methylated and the unmethylated alleles thereby increasing the sensitivity of the assays. Bisulfite treatment of the DNA prior to performing MS-HRM ensures a different base composition between methylated and unmethylated DNA, which is used to separate the resulting amplicons by high resolution melting.The high sensitivity of MS-HRM has proven useful for detecting cancer biomarkers in a noninvasive manner in urine from bladder cancer patients, in stool from colorectal cancer patients, and in buccal mucosa from breast cancer patients. MS-HRM is a fast method to diagnose imprinted diseases and to clinically validate results from whole-epigenome studies. The ability to detect few copies of methylated DNA makes MS-HRM a key player in the quest for establishing links between environmental exposure, epigenetic changes, and disease.

  2. Identification of six New World Leishmania species through the implementation of a High-Resolution Melting (HRM) genotyping assay.

    Science.gov (United States)

    Hernández, Carolina; Alvarez, Catalina; González, Camila; Ayala, Martha Stella; León, Cielo Maritza; Ramírez, Juan David

    2014-11-14

    Leishmaniases are tropical zoonotic diseases, caused by parasites from the genus Leishmania. New World (NW) species are related to sylvatic cycles although urbanization processes have been reported in some South American Countries such as Colombia. This eco-epidemiological complexity imposes a challenge to the detection of circulating parasite species, not only related to human cases but also infecting vectors and reservoirs. Currently, no harmonized methods have been deployed to discriminate the NW Leishmania species. Herein, we conducted a systematic and mechanistic High-Resolution Melting (HRM) assay targeted to HSP70 and ITS1. Specific primers were designed that coupled with a HRM analyses permitted to discriminate six NW Leishmania species. In order to validate the herein described algorithm, we included 35 natural isolates obtained from human cases, insect vectors and mammals. Our genotyping assay allowed the correct assignment of the six NW Leishmania species (L. mexicana, L. infantum (chagasi), L. amazonensis, L. panamensis, L. guyanensis and L. braziliensis) based on reference strains. When the algorithm was applied to a set of well-characterized strains by means of PCR-RFLP, MLEE and monoclonal antibodies (MA) we observed a tailored concordance between the HRM and PCR-RFLP/MLEE/MA (KI = 1.0). Additionally, we tested the limit of detection for the HRM method showing that this is able to detect at least 10 equivalent-parasites per mL. This is a rapid and reliable method to conduct molecular epidemiology and host-parasite association studies in endemic areas.

  3. e-HRM-user Perceptions of e-HRM Tools in Kuwaiti SME's

    NARCIS (Netherlands)

    AbuZained, Tahseen; Ruel, Huub J.M.; Ruel, H.J.M.; Magalhaes, R.

    2008-01-01

    This research aims to explore and analyze e-HRM users’ perceptions about e-HRM in SME’s in Kuwait. The research type will be explorative. The researcher believes that the research will help to acquire more information and knowledge about e-HRM in SMEs. Major researches of e-HRM have been conducted

  4. Developing HRM in Foreign Chinese Jount Ventures

    NARCIS (Netherlands)

    Verburg, R.M.

    1996-01-01

    Human Resource Management (HRM) has become established as an alternative perspective on personnel management. HRM takes many forms, but its basis is the link between personnel practices and business strategy leading to overall organizational effectiveness by encouraging strong commitment among

  5. HRM - that's in the other department!

    DEFF Research Database (Denmark)

    Brandl, Julia; Madsen, Mona Toft; Madsen, Henning

    2006-01-01

    HRM scholars have widely acknowledged that realizing HRM requires involvement of all managers; in the context of involving line management with HRM we need to understand how they perceive the importance of HRM and what relevance HRM related activities have in their daily business. In this paper we...... contribute to that literature by examining what importance managers give to HRM related activities in contrast to their other managerial activities and how male and female managers differ in their perception. Based on our analysis of 1.500 Danish managers we find that female managers show relatively higher...... relevance for HRM related activities. Finally, we discuss potential explanations for managers' perception of HRM compared with other activities....

  6. Uncovering configurations of HRM service provider intellectual capital and worker human capital for creating high HRM service value using fsQCA

    NARCIS (Netherlands)

    Meijerink, Jeroen Gerard; Bondarouk, Tatiana

    Although traditionally applied independently, this study combines two theoretical perspectives – the intellectual capital theory and the consumer perspective – to uncover value-creating configurations of human resource management (HRM) service providers' and workers' knowledge resources. We examined

  7. Identification case of evidence in timber tracing of Pinus radiate, using high-resolution melting (HRM) analysis.

    Science.gov (United States)

    Solano, Jaime; Anabalón, Leonardo; Encina, Francisco

    2016-03-01

    Fast, accurate detection of plant species and their hybrids using molecular tools will facilitate assessment and monitoring of timber tracing evidence. In this study the origin of unknown pine samples is determined for a case of timber theft in the region of Araucania southern Chile. We evaluate the utility of the trnL marker region for species identification applied to pine wood based on High Resolution Melting. This efficient tracing methods can be incorporated into forestry applications such as certification of origin. The object of this work was genotype identification using high-resolution melting (HRM) and trnL approaches for Pinus radiata (Don) in timber tracing evidence. Our results indicate that trnL is a very sensitive marker for delimiting species and HRM analysis was used successfully for genotyping Pinus samples for timber tracing purposes. Genotyping samples by HRM analysis with the trnL1 approach allowed us to differentiate two wood samples from the Pinaceae family: Pinus radiata (Don) and Pseudotsuga menziesii (Mirb.) Franco. The same approach with Pinus trnL wood was not able to discriminate between samples of Pinus radiata, indicating that the samples were genetically indistinguishable, possibly because they have the same genotype at this locus. Timber tracing with HRM analysis is expected to contribute to future forest certification schemes, control of illegal trading, and molecular traceability of Pinus spp. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  8. De kern van HRM. - 2e dr.

    NARCIS (Netherlands)

    Verburg, R.; den Hartog, D.

    2008-01-01

    In dit boek staat de kern van HRM centraal. Op basis van praktijkvoorbeelden, toonaangevende internationale publicaties en onderzoek in Nederland worden essentiële thema's van HRM belicht. Zo komen de volgende onderwerpen aan de orde: - De oorsprong van HRM - De veranderende relatie tussen werkgever

  9. High-resolution melting (HRM) re-analysis of a polyposis patients cohort reveals previously undetected heterozygous and mosaic APC gene mutations.

    Science.gov (United States)

    Out, Astrid A; van Minderhout, Ivonne J H M; van der Stoep, Nienke; van Bommel, Lysette S R; Kluijt, Irma; Aalfs, Cora; Voorendt, Marsha; Vossen, Rolf H A M; Nielsen, Maartje; Vasen, Hans F A; Morreau, Hans; Devilee, Peter; Tops, Carli M J; Hes, Frederik J

    2015-06-01

    Familial adenomatous polyposis is most frequently caused by pathogenic variants in either the APC gene or the MUTYH gene. The detection rate of pathogenic variants depends on the severity of the phenotype and sensitivity of the screening method, including sensitivity for mosaic variants. For 171 patients with multiple colorectal polyps without previously detectable pathogenic variant, APC was reanalyzed in leukocyte DNA by one uniform technique: high-resolution melting (HRM) analysis. Serial dilution of heterozygous DNA resulted in a lowest detectable allelic fraction of 6% for the majority of variants. HRM analysis and subsequent sequencing detected pathogenic fully heterozygous APC variants in 10 (6%) of the patients and pathogenic mosaic variants in 2 (1%). All these variants were previously missed by various conventional scanning methods. In parallel, HRM APC scanning was applied to DNA isolated from polyp tissue of two additional patients with apparently sporadic polyposis and without detectable pathogenic APC variant in leukocyte DNA. In both patients a pathogenic mosaic APC variant was present in multiple polyps. The detection of pathogenic APC variants in 7% of the patients, including mosaics, illustrates the usefulness of a complete APC gene reanalysis of previously tested patients, by a supplementary scanning method. HRM is a sensitive and fast pre-screening method for reliable detection of heterozygous and mosaic variants, which can be applied to leukocyte and polyp derived DNA.

  10. The Impact of 'Narco-terrorism' on HRM

    DEFF Research Database (Denmark)

    Ramirez, Jacobo; Madero, Sergio; Muñiz, Carlos

    This paper presents a mixed-method study that explores the configuration of human resource management (HRM) systems in “narco-terrorism” environments. The quantitative analysis focuses on 204 news stories. In addition, 25 interviews were conducted with managers and HR professionals at 20 firms...... in Mexico to explore the direct and indirect impacts of narco-terrorism on firms. Our analysis of firms’ responses in terms of HRM systems indicate that trust of formal and informal institutions seems to shape HRM system in the focal firms. More specifically, a combination of strict employee control......, an emphasis on soft-skills training and development, and a flexible work schedule seems to facilitate the building of trust in narco-terrorism contexts. It seems that the role of firms as social institutions integrate strategies, which seems to aim at avoid and manipulate the institutional constraints. Our...

  11. Vooral inhoud e-HRM toolbox verhoogt HR-effectiviteit

    NARCIS (Netherlands)

    Ruel, Hubertus Johannes Maria; Bondarouk, Tatiana

    2006-01-01

    Aanbieders van web-based HRM-applicaties claimen dat invoering van hun oplossingen HRM-processen efficiënter maakt, dus geld bespaart, medewerkers en management meer tevreden maakt over HRM-diensten en de HRM-afdeling meer tijd geeft voor strategische activiteiten. Kortom, HRM zou effectiever

  12. Applied Formal Methods for Elections

    DEFF Research Database (Denmark)

    Wang, Jian

    development time, or second dynamically, i.e. monitoring while an implementation is used during an election, or after the election is over, for forensic analysis. This thesis contains two chapters on this subject: the chapter Analyzing Implementations of Election Technologies describes a technique...... process. The chapter Measuring Voter Lines describes an automated data collection method for measuring voters' waiting time, and discusses statistical models designed to provide an understanding of the voter behavior in polling stations....

  13. Applied Formal Methods for Elections

    DEFF Research Database (Denmark)

    Wang, Jian

    Information technology is changing the way elections are organized. Technology renders the electoral process more efficient, but things could also go wrong: Voting software is complex, it consists of over thousands of lines of code, which makes it error-prone. Technical problems may cause delays...... bounded model-checking and satisfiability modulo theories (SMT) solvers can be used to check these criteria. Voter Experience: Technology profoundly affects the voter experience. These effects need to be measured and the data should be used to make decisions regarding the implementation of the electoral...... at polling stations, or even delay the announcement of the final result. This thesis describes a set of methods to be used, for example, by system developers, administrators, or decision makers to examine election technologies, social choice algorithms and voter experience. Technology: Verifiability refers...

  14. HRM Research in the Mainland China

    DEFF Research Database (Denmark)

    Cao, Dayou; Li, Peter Ping; Li, Yuanling

    2013-01-01

    as the emerging research trends by reviewing the literature in HRM research in the Mainland China. Design/methodology/approach – The paper takes a geocentric perspective of HRM theory development to analyze the status quo as well as the emerging trends of the future HRM research in the Mainland China. Findings......Purpose – The purpose of this perspective article is to identify the developmental trajectory of human resource management (HRM) research in the Mainland China as well as the major research gaps to be filled in the future. In particular, the paper focuses on the current challenges as well...... – HRM research in the Mainland China exhibited an obvious tendency of adopting an etic approach at the early stage of research, but displaying an emerging trend toward an emic approach at a later stage. However, the current HRM research in the Mainland China, including both etic and the emic approaches...

  15. Exploiting HRM in support of lean manufacturing

    DEFF Research Database (Denmark)

    Jørgensen, Frances; Matthiesen, Rikke

    The purpose of this paper is to investigate the ways in HRM practices are-and could potentially be-exploited to support lean manufacturing in practice. First, a review of the pertinent literature regarding HRM, SHRM, and lean manufacturing is presented to provide an understanding of the mechanisms...... by which HRM practices could, theoretically, be used to support a lean implementation. Data presented in the paper are derived from 1) a longitudinal case study on lean implementation and 2) from managers currently involved with lean manufacturing in a second company. The relevant literature and the data...... depicting the potential role in supporting HRM/lean integrated practices. The analysis of the model with respect to the theoretical background emphasizes a number of areas in which HRM could be more fully exploited in order to more successfully support lean implementation, for example, by stressing HRM...

  16. HRM bij Burgerzaken: Een empirisch onderzoek naar de effecten van HRM op de kostendoelmatigheid

    OpenAIRE

    Van Hulst, B.L.; De Groot, H.

    2012-01-01

    HRM bij Burgerzaken is een studie naar de effecten van HRM op de kostendoelmatigheid. In de studie zijn ingezette HRM-instrumenten gerelateerd aan de kostendoelmatigheid van de afdeling Burgerzaken. De studie geeft allereerst inzicht in de stand van zaken ten aanzien van HRM. Vervolgens is de kostendoelmatigheid in kaart gebracht, de gemiddelde doelmatigheidsscore in 2010 is 86,3%. Wel bestaan er duidelijke verschillen in kostendoelmatigheid tussen gemeenten die deels verklaard worden door de...

  17. Molecular differentiation of three canine and feline hookworms in South China through HRM analysis.

    Science.gov (United States)

    Wang, M W; Yan, X X; Hang, J X; Shi, X L; Fu, Y Q; Zhang, P; Yang, F; Pan, W D; Li, G Q

    2018-02-05

    To investigate the prevalence of canine and feline hookworms in South China, and to assess the risk of zoonotic hookworms to humans, one pair of primers (HRM-F/HRM-R) was designed to establish a high-resolution melting (HRM) method based on internal transcribed spacer 1 (ITS-1) rDNA for the detection of Ancylostoma ceylanicum, A. caninum and A. tubaeforme infection. The results showed that the HRM for the three hookworms produced different melting-curve profiles, where melting temperature (T m) values were 84.50°C for A. ceylanicum, 82.25°C for A. caninum and 81.73°C for A. tubaeforme, respectively. The reproducibility of intra- and inter-assay melting curves was almost perfect. The lowest concentration detected was about 5.69 ×10-4 g/μl. The HRM detection results from 18 canine and feline hookworm samples were in complete accordance with their sequencing results. The HRM method was more sensitive than the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique in the detection of 98 clinical samples. It is concluded that the HRM method can differentiate between A. ceylanicum, A. caninum, A. tubaeforme and their mixed infections, which may provide important technical support for the zoonotic risk assessment and molecular epidemiological survey of canine and feline hookworms.

  18. The dynamic nature of HRM implementation: a structuration perspective

    OpenAIRE

    van Mierlo, Jorrit; Bondarouk, Tanya; Sanders, Karin

    2018-01-01

    Over the past decades, scholars have dedicated substantial attention to the process of HRM implementation. Most progress has been made with debates on HRM system strength, roles of organisational actors in HRM implementation, and intended, actual and perceived HRM. In this paper, we challenge the current view on HRM implementation as being too static and one-directional. By building on structuration theory, we show that the process of HRM implementation is less straightforward than has been p...

  19. A Novel Multiplex HRM Assay to Detect Clopidogrel Resistance.

    Science.gov (United States)

    Zhang, Lichen; Ma, Xiaowei; You, Guoling; Zhang, Xiaoqing; Fu, Qihua

    2017-11-22

    Clopidogrel is an antiplatelet medicine used to prevent blood clots in patients who have had a heart attack, stroke, or other symptoms. Variability in the clinical response to clopidogrel treatment has been attributed to genetic factors. In particular, five SNPs of rs4244285, rs4986893, rs12248560, rs662 and rs1045642 have been associated with resistance to clopidogrel therapy in Chinese population. This work involves the development of a multiplex high-resolution melting (HRM) assay to genotype all five of these loci in 2 tubes. Amplicons corresponding to distinct SNPs in a common tube were designed with the aid of uMelt prediction software to have different melting temperatures Tm by addition of a GC-rich tail to the 5' end of the certain primers. Two kinds of commercial methods, Digital Fluorescence Molecular Hybridization (DFMH) and Sanger sequencing, were used as a control. Three hundred sixteen DFMH pretested samples from consecutive acute coronary syndrome patients were used for a blinded study of multiplex HRM. The sensitivity of HRM was 100% and the specificity was 99.93% reflecting detection of variants other than the known resistance SNPs. Multiplex HRM is an effective closed-tube, highly accurate, fast, and inexpensive method for genotyping the 5 clopidogrel resistance associated SNPs.

  20. Using SSR-HRM to Identify Closely Related Species in Herbal Medicine Products: A Case Study on Licorice.

    Science.gov (United States)

    Li, Jingjian; Xiong, Chao; He, Xia; Lu, Zhaocen; Zhang, Xin; Chen, Xiaoyang; Sun, Wei

    2018-01-01

    Traditional herbal medicines have played important roles in the ways of life of people around the world since ancient times. Despite the advanced medical technology of the modern world, herbal medicines are still used as popular alternatives to synthetic drugs. Due to the increasing demand for herbal medicines, plant species identification has become an important tool to prevent substitution and adulteration. Here we propose a method for biological assessment of the quality of prescribed species in the Chinese Pharmacopoeia by use of high resolution melting (HRM) analysis of microsatellite loci. We tested this method on licorice, a traditional herbal medicine with a long history. Results showed that nine simple sequence repeat (SSR) markers produced distinct melting curve profiles for the five licorice species investigated using HRM analysis. These results were validated by capillary electrophoresis. We applied this protocol to commercially available licorice products, thus enabling the consistent identification of 11 labels with non-declared Glycyrrhiza species. This novel strategy may thus facilitate DNA barcoding as a method of identification of closely related species in herbal medicine products. Based on this study, a brief operating procedure for using the SSR-HRM protocol for herbal authentication is provided.

  1. Detection of somatic mutations by high-resolution DNA melting (HRM) analysis in multiple cancers.

    Science.gov (United States)

    Gonzalez-Bosquet, Jesus; Calcei, Jacob; Wei, Jun S; Garcia-Closas, Montserrat; Sherman, Mark E; Hewitt, Stephen; Vockley, Joseph; Lissowska, Jolanta; Yang, Hannah P; Khan, Javed; Chanock, Stephen

    2011-01-17

    Identification of somatic mutations in cancer is a major goal for understanding and monitoring the events related to cancer initiation and progression. High resolution melting (HRM) curve analysis represents a fast, post-PCR high-throughput method for scanning somatic sequence alterations in target genes. The aim of this study was to assess the sensitivity and specificity of HRM analysis for tumor mutation screening in a range of tumor samples, which included 216 frozen pediatric small rounded blue-cell tumors as well as 180 paraffin-embedded tumors from breast, endometrial and ovarian cancers (60 of each). HRM analysis was performed in exons of the following candidate genes known to harbor established commonly observed mutations: PIK3CA, ERBB2, KRAS, TP53, EGFR, BRAF, GATA3, and FGFR3. Bi-directional sequencing analysis was used to determine the accuracy of the HRM analysis. For the 39 mutations observed in frozen samples, the sensitivity and specificity of HRM analysis were 97% and 87%, respectively. There were 67 mutation/variants in the paraffin-embedded samples, and the sensitivity and specificity for the HRM analysis were 88% and 80%, respectively. Paraffin-embedded samples require higher quantity of purified DNA for high performance. In summary, HRM analysis is a promising moderate-throughput screening test for mutations among known candidate genomic regions. Although the overall accuracy appears to be better in frozen specimens, somatic alterations were detected in DNA extracted from paraffin-embedded samples.

  2. Detection of somatic mutations by high-resolution DNA melting (HRM analysis in multiple cancers.

    Directory of Open Access Journals (Sweden)

    Jesus Gonzalez-Bosquet

    Full Text Available Identification of somatic mutations in cancer is a major goal for understanding and monitoring the events related to cancer initiation and progression. High resolution melting (HRM curve analysis represents a fast, post-PCR high-throughput method for scanning somatic sequence alterations in target genes. The aim of this study was to assess the sensitivity and specificity of HRM analysis for tumor mutation screening in a range of tumor samples, which included 216 frozen pediatric small rounded blue-cell tumors as well as 180 paraffin-embedded tumors from breast, endometrial and ovarian cancers (60 of each. HRM analysis was performed in exons of the following candidate genes known to harbor established commonly observed mutations: PIK3CA, ERBB2, KRAS, TP53, EGFR, BRAF, GATA3, and FGFR3. Bi-directional sequencing analysis was used to determine the accuracy of the HRM analysis. For the 39 mutations observed in frozen samples, the sensitivity and specificity of HRM analysis were 97% and 87%, respectively. There were 67 mutation/variants in the paraffin-embedded samples, and the sensitivity and specificity for the HRM analysis were 88% and 80%, respectively. Paraffin-embedded samples require higher quantity of purified DNA for high performance. In summary, HRM analysis is a promising moderate-throughput screening test for mutations among known candidate genomic regions. Although the overall accuracy appears to be better in frozen specimens, somatic alterations were detected in DNA extracted from paraffin-embedded samples.

  3. HRM and Fit: Survival of the fittest!?

    NARCIS (Netherlands)

    C.T. Boon (Corine)

    2008-01-01

    textabstractWhat is the nature of ‘fit’ in strategic HRM? This is the question guiding this thesis. Although the idea of fit is theoretically convincing, empirical evidence to support the role of fit is limited. Previous studies on fit have mainly focused on the alignment between HRM and strategy

  4. High-resolution melting analysis (HRM) for differentiation of four major Taeniidae species in dogs Taenia hydatigena, Taenia multiceps, Taenia ovis, and Echinococcus granulosus sensu stricto.

    Science.gov (United States)

    Dehghani, Mansoureh; Mohammadi, Mohammad Ali; Rostami, Sima; Shamsaddini, Saeedeh; Mirbadie, Seyed Reza; Harandi, Majid Fasihi

    2016-07-01

    Tapeworms of the genus Taenia include several species of important parasites with considerable medical and veterinary significance. Accurate identification of these species in dogs is the prerequisite of any prevention and control program. Here, we have applied an efficient method for differentiating four major Taeniid species in dogs, i.e., Taenia hydatigena, T. multiceps, T. ovis, and Echinococcus granulosus sensu stricto. High-resolution melting (HRM) analysis is simpler, less expensive, and faster technique than conventional DNA-based assays and enables us to detect PCR amplicons in a closed system. Metacestode samples were collected from local abattoirs from sheep. All the isolates had already been identified by PCR-sequencing, and their sequence data were deposited in the GenBank. Real-time PCR coupled with HRM analysis targeting mitochondrial cox1 and ITS1 genes was used to differentiate taeniid species. Distinct melting curves were obtained from ITS1 region enabling accurate differentiation of three Taenia species and E. granulosus in dogs. The HRM curves of Taenia species and E .granulosus were clearly separated at Tm of 85 to 87 °C. In addition, double-pick melting curves were produced in mixed infections. Cox1 melting curves were not decisive enough to distinguish four taeniids. In this work, the efficiency of HRM analysis to differentiate four major taeniid species in dogs has been demonstrated using ITS1 gene.

  5. HRM og innovation i vidensintensive virksomheder

    DEFF Research Database (Denmark)

    Jørgensen, Frances; Becker, Karen; Matthews, Judith

    2010-01-01

    Formålet med denne artikel er at identificere de HRM-praksisser, der understøtter innovation. Artiklen gennemgår kort den internationale litteratur, der er relevant for HRM og innovation i videnstunge virksomheder og bringer endvidere erfaringer fra fire case studier fra videnstunge virksomheder i...... Danmark og Australien, der alle er anerkendte for deres innovationskapacitet. Resultaterne fra dette studium indikerer, at mens der er fællestræk mellem HRM praksisser inden for traditionelle produktionsvirksomheder og videnstunge virksomheder, er der også vigtige forskelle især med hensyn til...... fremgangsmåder omkring bemanding. Artiklen bringer endvidere anbefalinger til HRM-ledelse i innovative videnstunge virksomheder og skitserer mulige veje for forskning for yderligere at udvikle vores forståelse for, hvordan HRM kan støtte innovation i videnstunge virksomheder....

  6. e-HRM Research and Practice: Facing the Challenges Ahead

    NARCIS (Netherlands)

    Ruel, Hubertus Johannes Maria; Bondarouk, Tatiana; Martinez-Lopez, Francisco J.

    2014-01-01

    The history of e-HRM research extends back about 4 decades. In that time, researchers have provided a rich foundation for a better understanding of issues such as e-HRM implementation, e-HRM usage, and e-HRM outcomes. The past decade in particular saw an impressive growth of publications, but more

  7. The dynamic nature of HRM implementation : a structuration perspective

    NARCIS (Netherlands)

    van Mierlo, Jorrit; Bondarouk, Tanya; Sanders, Karin

    2018-01-01

    Over the past decades, scholars have dedicated substantial attention to the process of HRM implementation. Most progress has been made with debates on HRM system strength, roles of organisational actors in HRM implementation, and intended, actual and perceived HRM. In this paper, we challenge the

  8. e-HRM kan bibringe organisationen værdi

    DEFF Research Database (Denmark)

    Holm, Anna

    2009-01-01

    Elektronisk HRM (e-HRM), der tit også kaldes virtuel HRM, er i stigende grad en del af større organisationer, men der mangler ofte en klar difinition af begrebet. I denne artikel får du styr på begreber som e-HRM, IKT og TMS....

  9. [Novel Approaches in DNA Methylation Studies - MS-HRM Analysis and Electrochemistry].

    Science.gov (United States)

    Bartošík, M; Ondroušková, E

    Cytosine methylation in DNA is an epigenetic mechanism regulating gene expression and plays a vital role in cell differentiation or proliferation. Tumor cells often exhibit aberrant DNA methylation, e.g. hypermethylation of tumor suppressor gene promoters. New methods, capable of determining methylation status of specific DNA sequences, are thus being developed. Among them, MS-HRM (methylation-specific high resolution melting) and electrochemistry offer relatively inexpensive instrumentation, fast assay times and possibility of screening multiple samples/DNA regions simultaneously. MS-HRM is due to its sensitivity and simplicity an interesting alternative to already established techniques, including methylation-specific PCR or bisulfite sequencing. Electrochemistry, when combined with suitable electroactive labels and electrode surfaces, has been applied in several unique strategies for discrimination of cytosines and methylcytosines. Both techniques were successfully tested in analysis of DNA methylation within promoters of important tumor suppressor genes and could thus help in achieving more precise diagnostics and prognostics of cancer. Aberrant methylation of promoters has already been described in hundreds of genes associated with tumorigenesis and could serve as important biomarker if new methods applicable into clinical practice are sufficiently advanced.Key words: DNA methylation - 5-methylcytosine - HRM analysis - melting temperature - DNA duplex - electrochemistry - nucleic acid hybridizationThis work was supported by MEYS - NPS I - LO1413.The authors declare they have no potential conflicts of interest concerning drugs, products, or services used in the study.The Editorial Board declares that the manuscript met the ICMJE recommendation for biomedical papers.Submitted: 6. 5. 2016Accepted: 16. 5. 2016.

  10. Fast and reliable detection of toxic Crotalaria spectabilis Roth. in Thunbergia laurifolia Lindl. herbal products using DNA barcoding coupled with HRM analysis.

    Science.gov (United States)

    Singtonat, Sahachat; Osathanunkul, Maslin

    2015-05-30

    Nowadays, medicinal plants are used as a popular alternative to synthetic drugs. Many medicinal plant products have now been commercialized throughout various markets. These products are commonly sold in processed or modified forms such as powders, dried material and capsules, making it almost impossible to accurately identify the constituent species. The herbal plant known as 'Rang Chuet' in Thai has been widely used as remedies for various ailments. However, two medicinal plants species, Thunbergia laurifolia and Crotalaria spectabilis share this name. Duo to the similarity in nomenclature, the commercial products labeled as 'Rang Chuet' could be any of them. Recently, the evidence of hepatotoxic effects linked to use of C. spectabilis were reported and is now seriously concern. There is a need to find an approach that could help with species identification of these herbal products to ensure the safety and efficacy of the herbal drug. Here DNA barcoding was used in combination with High Resolution Melting analysis (Bar-HRM) to authenticate T. laurifolia species. Four DNA barcodes including matK, rbcL, rpoC and trnL were selected for use in primers design for HRM analysis to produce standard melting profiles of the selected species. Commercial products labeled as 'Rang Chuet' were purchased from Thai markets and authentication by HRM analyses. Melting data from the HRM assay using the designed primers showed that the two 'Rang Chuet' species could easily be distinguished from each other. The melting profiles of the all four region amplicons of each species are clearly separated in all three replicates. The method was then applied to authenticate products in powdered form. HRM curves of all ten test samples indicated that three of the tested products did not only contain the T. laurifolia species. The herbal drugs derived from different plants must be distinguished from each other even they share the same vernacular name. The Bar-HRM method developed here proved

  11. A comparison of PCR assays for beak and feather disease virus and high resolution melt (HRM) curve analysis of replicase associated protein and capsid genes.

    Science.gov (United States)

    Das, Shubhagata; Sarker, Subir; Ghorashi, Seyed Ali; Forwood, Jade K; Raidal, Shane R

    2016-11-01

    Beak and feather disease virus (BFDV) threatens a wide range of endangered psittacine birds worldwide. In this study, we assessed a novel PCR assay and genetic screening method using high-resolution melt (HRM) curve analysis for BFDV targeting the capsid (Cap) gene (HRM-Cap) alongside conventional PCR detection as well as a PCR method that targets a much smaller fragment of the virus genome in the replicase initiator protein (Rep) gene (HRM-Rep). Limits of detection, sensitivity, specificity and discriminatory power for differentiating BFDV sequences were compared. HRM-Cap had a high positive predictive value and could readily differentiate between a reference genotype and 17 other diverse BFDV genomes with more discriminatory power (genotype confidence percentage) than HRM-Rep. Melt curve profiles generated by HRM-Cap correlated with unique DNA sequence profiles for each individual test genome. The limit of detection of HRM-Cap was lower (2×10 -5 ng/reaction or 48 viral copies) than that for both HRM-Rep and conventional BFDV PCR which had similar sensitivity (2×10 -6 ng or 13 viral copies/reaction). However, when used in a diagnostic setting with 348 clinical samples there was strong agreement between HRM-Cap and conventional PCR (kappa=0.87, PHRM-Cap demonstrated higher specificity (99.9%) than HRM-Rep (80.3%). Copyright © 2016 Elsevier B.V. All rights reserved.

  12. Non-invasive prenatal diagnosis of multiple endocrine neoplasia type 2A using COLD-PCR combined with HRM genotyping analysis from maternal serum.

    Science.gov (United States)

    Macher, Hada C; Martinez-Broca, Maria A; Rubio-Calvo, Amalia; Leon-Garcia, Cristina; Conde-Sanchez, Manuel; Costa, Alzenira; Navarro, Elena; Guerrero, Juan M

    2012-01-01

    The multiple endocrine neoplasia type 2A (MEN2A) is a monogenic disorder characterized by an autosomal dominant pattern of inheritance which is characterized by high risk of medullary thyroid carcinoma in all mutation carriers. Although this disorder is classified as a rare disease, the patients affected have a low life quality and a very expensive and continuous treatment. At present, MEN2A is diagnosed by gene sequencing after birth, thus trying to start an early treatment and by reduction of morbidity and mortality. We first evaluated the presence of MEN2A mutation (C634Y) in serum of 25 patients, previously diagnosed by sequencing in peripheral blood leucocytes, using HRM genotyping analysis. In a second step, we used a COLD-PCR approach followed by HRM genotyping analysis for non-invasive prenatal diagnosis of a pregnant woman carrying a fetus with a C634Y mutation. HRM analysis revealed differences in melting curve shapes that correlated with patients diagnosed for MEN2A by gene sequencing analysis with 100% accuracy. Moreover, the pregnant woman carrying the fetus with the C634Y mutation revealed a melting curve shape in agreement with the positive controls in the COLD-PCR study. The mutation was confirmed by sequencing of the COLD-PCR amplification product. In conclusion, we have established a HRM analysis in serum samples as a new primary diagnosis method suitable for the detection of C634Y mutations in MEN2A patients. Simultaneously, we have applied the increase of sensitivity of COLD-PCR assay approach combined with HRM analysis for the non-invasive prenatal diagnosis of C634Y fetal mutations using pregnant women serum.

  13. Non-invasive prenatal diagnosis of multiple endocrine neoplasia type 2A using COLD-PCR combined with HRM genotyping analysis from maternal serum.

    Directory of Open Access Journals (Sweden)

    Hada C Macher

    Full Text Available The multiple endocrine neoplasia type 2A (MEN2A is a monogenic disorder characterized by an autosomal dominant pattern of inheritance which is characterized by high risk of medullary thyroid carcinoma in all mutation carriers. Although this disorder is classified as a rare disease, the patients affected have a low life quality and a very expensive and continuous treatment. At present, MEN2A is diagnosed by gene sequencing after birth, thus trying to start an early treatment and by reduction of morbidity and mortality. We first evaluated the presence of MEN2A mutation (C634Y in serum of 25 patients, previously diagnosed by sequencing in peripheral blood leucocytes, using HRM genotyping analysis. In a second step, we used a COLD-PCR approach followed by HRM genotyping analysis for non-invasive prenatal diagnosis of a pregnant woman carrying a fetus with a C634Y mutation. HRM analysis revealed differences in melting curve shapes that correlated with patients diagnosed for MEN2A by gene sequencing analysis with 100% accuracy. Moreover, the pregnant woman carrying the fetus with the C634Y mutation revealed a melting curve shape in agreement with the positive controls in the COLD-PCR study. The mutation was confirmed by sequencing of the COLD-PCR amplification product. In conclusion, we have established a HRM analysis in serum samples as a new primary diagnosis method suitable for the detection of C634Y mutations in MEN2A patients. Simultaneously, we have applied the increase of sensitivity of COLD-PCR assay approach combined with HRM analysis for the non-invasive prenatal diagnosis of C634Y fetal mutations using pregnant women serum.

  14. [Montessori method applied to dementia - literature review].

    Science.gov (United States)

    Brandão, Daniela Filipa Soares; Martín, José Ignacio

    2012-06-01

    The Montessori method was initially applied to children, but now it has also been applied to people with dementia. The purpose of this study is to systematically review the research on the effectiveness of this method using Medical Literature Analysis and Retrieval System Online (Medline) with the keywords dementia and Montessori method. We selected lo studies, in which there were significant improvements in participation and constructive engagement, and reduction of negative affects and passive engagement. Nevertheless, systematic reviews about this non-pharmacological intervention in dementia rate this method as weak in terms of effectiveness. This apparent discrepancy can be explained because the Montessori method may have, in fact, a small influence on dimensions such as behavioral problems, or because there is no research about this method with high levels of control, such as the presence of several control groups or a double-blind study.

  15. The Transfer of HRM Policies and Practices in American Multinational Hotels in Saudi Arabia

    Directory of Open Access Journals (Sweden)

    Amal Hatem Alkhaldi

    2014-12-01

    Full Text Available Global competition in the international business environment has pushed companies to achieve competitive advantage through mergers, acquisitions and through locating their subsidiaries in less developed counties for cost effectiveness. Consequently, the competitive pressure has increased the significance of human resource management (HRM in multinational companies (MNCs, and MNCs have recognised the significance of the transfer HRM process across borders. This study examines the transfer of HRM policies and practices of US MNCs to their subsidiaries in Saudi Arabia. The aim of the study is to determine the extent to which US MNCs transfer HRM policies and practices from their Headquarters to their subsidiaries in Saudi Arabia; and identify the factors that facilitate and inhibit HRM transfer. The paper is based on an investigation of the interaction between home-country and host-country effects in determining HRM policies and practices in MNCs in the context of the Middle East. The study adopts a mixed methods approach of documentary analysis, focus group interviews of employees and in depth interviews of key informants in four subsidiaries of US owned international hotel chains located in Riyadh, Saudi Arabia.

  16. The impact of HRM practices on supply chain management success in SME

    Directory of Open Access Journals (Sweden)

    Naveed R. Khan

    2013-09-01

    Full Text Available Background: Management practices are being carried out by most of the small and medium sized enterprises (SME which substantially switched to sophisticated manner. This study aims to determine the relationship between human resource management (HRM and supply chain management (SCM in SME. Methods: the data from 195 manufacturing and service sectors SME were collected.  The Pearson's correlation and multiple regression were employed to examine the relationship and measure the overall impact of IV on DV respectively. This study found that SME performed moderate level of HRM and SCM practices and there is a correlation between HRM practices that proactively contributes in supply chain success. Lastly, training contributed greater to SCM success as compared to other HRM factors. Results and conclusions: Results suggest that SCM success is activated by HRM practices.  SME owners/managers should focus on enhancing the SCM success by implementing sophisticated HRM practices. This integration will allow mapping unique strategies to gain an edge over competitors. Appropriate approaches should be considered at national level to boost the national economy through SME sector.

  17. Genetic high throughput screening in Retinitis Pigmentosa based on high resolution melting (HRM) analysis.

    Science.gov (United States)

    Anasagasti, Ander; Barandika, Olatz; Irigoyen, Cristina; Benitez, Bruno A; Cooper, Breanna; Cruchaga, Carlos; López de Munain, Adolfo; Ruiz-Ederra, Javier

    2013-11-01

    Retinitis Pigmentosa (RP) involves a group of genetically determined retinal diseases caused by a large number of mutations that result in rod photoreceptor cell death followed by gradual death of cone cells. Most cases of RP are monogenic, with more than 80 associated genes identified so far. The high number of genes and variants involved in RP, among other factors, is making the molecular characterization of RP a real challenge for many patients. Although HRM has been used for the analysis of isolated variants or single RP genes, as far as we are concerned, this is the first study that uses HRM analysis for a high-throughput screening of several RP genes. Our main goal was to test the suitability of HRM analysis as a genetic screening technique in RP, and to compare its performance with two of the most widely used NGS platforms, Illumina and PGM-Ion Torrent technologies. RP patients (n = 96) were clinically diagnosed at the Ophthalmology Department of Donostia University Hospital, Spain. We analyzed a total of 16 RP genes that meet the following inclusion criteria: 1) size: genes with transcripts of less than 4 kb; 2) number of exons: genes with up to 22 exons; and 3) prevalence: genes reported to account for, at least, 0.4% of total RP cases worldwide. For comparison purposes, RHO gene was also sequenced with Illumina (GAII; Illumina), Ion semiconductor technologies (PGM; Life Technologies) and Sanger sequencing (ABI 3130xl platform; Applied Biosystems). Detected variants were confirmed in all cases by Sanger sequencing and tested for co-segregation in the family of affected probands. We identified a total of 65 genetic variants, 15 of which (23%) were novel, in 49 out of 96 patients. Among them, 14 (4 novel) are probable disease-causing genetic variants in 7 RP genes, affecting 15 patients. Our HRM analysis-based study, proved to be a cost-effective and rapid method that provides an accurate identification of genetic RP variants. This approach is effective for

  18. Geostatistical methods applied to field model residuals

    DEFF Research Database (Denmark)

    Maule, Fox; Mosegaard, K.; Olsen, Nils

    consists of measurement errors and unmodelled signal), and is typically assumed to be uncorrelated and Gaussian distributed. We have applied geostatistical methods to analyse the residuals of the Oersted(09d/04) field model [http://www.dsri.dk/Oersted/Field_models/IGRF_2005_candidates/], which is based...

  19. The spa typing of methicillin-resistant Staphylococcus aureus isolates by High Resolution Melting (HRM) analysis.

    Science.gov (United States)

    Fasihi, Yasser; Fooladi, Saba; Mohammadi, Mohammad Ali; Emaneini, Mohammad; Kalantar-Neyestanaki, Davood

    2017-09-06

    Molecular typing is an important tool for control and prevention of infection. A suitable molecular typing method for epidemiological investigation must be easy to perform, highly reproducible, inexpensive, rapid and easy to interpret. In this study, two molecular typing methods including the conventional PCR-sequencing method and high resolution melting (HRM) analysis were used for staphylococcal protein A (spa) typing of 30 Methicillin-resistant Staphylococcus aureus (MRSA) isolates recovered from clinical samples. Based on PCR-sequencing method results, 16 different spa types were identified among the 30 MRSA isolates. Among the 16 different spa types, 14 spa types separated by HRM method. Two spa types including t4718 and t2894 were not separated from each other. According to our results, spa typing based on HRM analysis method is very rapid, easy to perform and cost-effective, but this method must be standardized for different regions, spa types, and real-time machinery.

  20. Applied mathematical methods in nuclear thermal hydraulics

    International Nuclear Information System (INIS)

    Ransom, V.H.; Trapp, J.A.

    1983-01-01

    Applied mathematical methods are used extensively in modeling of nuclear reactor thermal-hydraulic behavior. This application has required significant extension to the state-of-the-art. The problems encountered in modeling of two-phase fluid transients and the development of associated numerical solution methods are reviewed and quantified using results from a numerical study of an analogous linear system of differential equations. In particular, some possible approaches for formulating a well-posed numerical problem for an ill-posed differential model are investigated and discussed. The need for closer attention to numerical fidelity is indicated

  1. Entropy viscosity method applied to Euler equations

    International Nuclear Information System (INIS)

    Delchini, M. O.; Ragusa, J. C.; Berry, R. A.

    2013-01-01

    The entropy viscosity method [4] has been successfully applied to hyperbolic systems of equations such as Burgers equation and Euler equations. The method consists in adding dissipative terms to the governing equations, where a viscosity coefficient modulates the amount of dissipation. The entropy viscosity method has been applied to the 1-D Euler equations with variable area using a continuous finite element discretization in the MOOSE framework and our results show that it has the ability to efficiently smooth out oscillations and accurately resolve shocks. Two equations of state are considered: Ideal Gas and Stiffened Gas Equations Of State. Results are provided for a second-order time implicit schemes (BDF2). Some typical Riemann problems are run with the entropy viscosity method to demonstrate some of its features. Then, a 1-D convergent-divergent nozzle is considered with open boundary conditions. The correct steady-state is reached for the liquid and gas phases with a time implicit scheme. The entropy viscosity method correctly behaves in every problem run. For each test problem, results are shown for both equations of state considered here. (authors)

  2. Analytical methods applied to water pollution

    International Nuclear Information System (INIS)

    Baudin, G.

    1977-01-01

    A comparison of different methods applied to water analysis is given. The discussion is limited to the problems presented by inorganic elements, accessible to nuclear activation analysis methods. The following methods were compared: activation analysis: with gamma-ray spectrometry, atomic absorption spectrometry, fluorimetry, emission spectrometry, colorimetry or spectrophotometry, X-ray fluorescence, mass spectrometry, voltametry, polarography or other electrochemical methods, activation analysis-beta measurements. Drinking-water, irrigation waters, sea waters, industrial wastes and very pure waters are the subjects of the investigations. The comparative evaluation is made on the basis of storage of samples, in situ analysis, treatment and concentration, specificity and interference, monoelement or multielement analysis, analysis time and accuracy. The significance of the neutron analysis is shown. (T.G.)

  3. Identification of Uvaria sp by barcoding coupled with high-resolution melting analysis (Bar-HRM).

    Science.gov (United States)

    Osathanunkul, M; Madesis, P; Ounjai, S; Pumiputavon, K; Somboonchai, R; Lithanatudom, P; Chaowasku, T; Wipasa, J; Suwannapoom, C

    2016-01-13

    DNA barcoding, which was developed about a decade ago, relies on short, standardized regions of the genome to identify plant and animal species. This method can be used to not only identify known species but also to discover novel ones. Numerous sequences are stored in online databases worldwide. One of the ways to save cost and time (by omitting the sequencing step) in species identification is to use available barcode data to design optimized primers for further analysis, such as high-resolution melting analysis (HRM). This study aimed to determine the effectiveness of the hybrid method Bar-HRM (DNA barcoding combined with HRM) to identify species that share similar external morphological features, rather than conduct traditional taxonomic identification that require major parts (leaf, flower, fruit) of the specimens. The specimens used for testing were those, which could not be identified at the species level and could either be Uvaria longipes or Uvaria wrayias, indicated by morphological identification. Primer pairs derived from chloroplast regions (matK, psbA-trnH, rbcL, and trnL) were used in the Bar-HRM. The results obtained from psbA-trnH primers were good enough to help in identifying the specimen while the rest were not. Bar-HRM analysis was proven to be a fast and cost-effective method for plant species identification.

  4. HRM implementation in multinational companies : the dynamics of multifaceted scenarios

    NARCIS (Netherlands)

    Bos-Nehles, Anna; Bondarouk, Tanya; Labrenz, Soren

    2017-01-01

    This study explores why the subsidiary line managers of multinational companies (MNCs) implement HRM practices differently than intended by headquarters. HRM implementation is understood as a process in which one has to differentiate between a range of multifaceted HRM implementation scenarios. We

  5. The Potential Power of Bar-HRM Technology in Herbal Medicine Identification.

    Science.gov (United States)

    Sun, Wei; Li, Jing-Jian; Xiong, Chao; Zhao, Bo; Chen, Shi-Lin

    2016-01-01

    The substitution of low-cost or adulterated herbal products for high-priced herbs makes it important to be able to identify and trace herbal plant species and their processed products in the drug supply chain. PCR-based methods play an increasing role in monitoring the safety of herbal medicines by detecting adulteration. Recent studies have shown the potential of DNA barcoding combined with high resolution melting (Bar-HRM) analysis in herbal medicine identification. This method involves precisely monitoring the change in fluorescence caused by the release of an intercalating DNA dye from a DNA duplex as it is denatured by a gradual increase in temperature. Since the melting profile depends on the GC content, length, and strand complementarity of the amplification product, Bar-HRM analysis opens up the possibility of detecting single-base variants or species-specific differences in a short region of DNA. This review summarizes key factors affecting Bar-HRM analysis and describes how Bar-HRM is performed. We then discuss advances in Bar-HRM analysis of medicinal plant ingredients (herbal materia medica) as a contribution toward safe and effective herbal medicines.

  6. Using Social Network Research in HRM

    DEFF Research Database (Denmark)

    Kaše, Robert; King, Zella; Minbaeva, Dana

    2013-01-01

    ; the impact of social networking sites on perceptions of relationships; and ethical issues in organizational network analysis, we propose specific suggestions to bring social network perspectives closer to HRM researchers and practitioners and rebalance our attention to people and to their relationships.......The article features a conversation between Rob Cross and Martin Kilduff about organizational network analysis in research and practice. It demonstrates the value of using social network perspectives in HRM. Drawing on the discussion about managing personal networks; managing the networks of others...

  7. The contribution of e-HRM to HRM effectiveness. Results from a quantitative study in a Dutch Ministry.

    NARCIS (Netherlands)

    Ruel, Hubertus Johannes Maria; Bondarouk, Tatiana; van der Velde, Mandy

    2007-01-01

    Purpose – Both for-profit and not-for-profit organisations have been replacing face-to-face HRM activities with web-based HRM tools, e-HRM for short, for employees and managers since the 1990s. This paper aims to look at whether this is of benefit to an organisation. Design/methodology/approach –

  8. E-HRM: Innovation or irritation. An explorative empirical study in five large companies on web-based HRM

    NARCIS (Netherlands)

    Ruel, Hubertus Johannes Maria; Bondarouk, Tatiana; Looise, Jan C.

    2004-01-01

    Technological optimistic voices assume that, from a technical perspective, the IT possibilities for HRM are endless: in principal all HR processes can be supported by IT. E-HRM is the relatively new term for this IT supported HRM, especially through the use of web technology. This paper aims at

  9. Authenticity analyses of Rhizoma Paridis using barcoding coupled with high resolution melting (Bar-HRM) analysis to control its quality for medicinal plant product.

    Science.gov (United States)

    Duan, Bao-Zhong; Wang, Ya-Ping; Fang, Hai-Lan; Xiong, Chao; Li, Xi-Wen; Wang, Ping; Chen, Shi-Lin

    2018-01-01

    Rhizoma Paridis (Chonglou) is a commonly used and precious traditional Chinese medicine. Paris polyphylla Smith var. yunnanensis (Franch.) Hand. -Mazz. and Paris polyphylla Smith var . chinensis (Franch.) Hara are the two main sources of Chonglou under the monograph of Rhizoma Paridis in Chinese Pharmacopoeia. In the local marketplace, however, this medicine is prone to be accidentally contaminated, deliberately substituted or admixed with other species that are similar to Rhizoma Paridis in shape and color. Consequently, these adulterations might compromise quality control and result in considerable health concerns for consumers. This study aims to develop a rapid and sensitive method for accurate identification of Rhizoma Paridis and its common adulterants. DNA barcoding coupled with high resolution melting analysis was applied in this research to distinguish Rhizoma Paridis from its adulteration. The internal transcribed spacer 2 (ITS2) barcode was selected for HRM analysis to produce standard melting profile of the selected species. DNA of the tested herbal medicines was isolated and their melting profiles were generated and compared with the standard melting profile of P. polyphylla var. chinensis . The results indicate that the ITS2 molecular regions coupled with HRM analysis can effectively differentiate nine herbal species, including two authentic origins of Chonglou and their seven common adulterants. Ten herbal medicines labeled "Chonglou" obtained from a local market were collected and identified with our methods, and their sequence information was analyzed to validate the accuracy of HRM analysis. DNA barcoding coupled with HRM analysis is a accurate, reliable, rapid, cost-effective and robust tool, which could contribute to the quality control of Rhizoma Paridis in the supply chain of the natural health product industry (NHP).

  10. Employees as Active Consumers of HRM: Linking Employees’ HRM Competences with Their Perceptions of HRM Service Value

    NARCIS (Netherlands)

    Meijerink, Jeroen Gerard; Bondarouk, Tatiana; Lepak, David P.

    2016-01-01

    Recognizing the importance for companies of having high-quality employment relationships with employees, previous studies have sought to explain the variability in employees’ perceptions of HRM service value. However, most of these studies view employees as inactive in employment relationships and,

  11. UOP HRM 300 Final Exam Guide (New, 2017) NEW

    OpenAIRE

    ADMIN

    2018-01-01

    UOP HRM 300 Final Exam Guide (New, 2017) NEW Check this A+ tutorial guideline at http://www.hrm300assignment.com/hrm-300-uop/hrm-300-final-exam-guide-latest For more classes visit http://www.hrm300assignment.com True and False: Please mark either T or F in the appropriate space before the question 1. ____A strategic plan is the company’s plan for how it will match its internal strengths and weaknesses with external opportunities and threats in or...

  12. Developing and Evaluating the HRM Technique for Identifying Cytochrome P450 2D6 Polymorphisms.

    Science.gov (United States)

    Lu, Hsiu-Chin; Chang, Ya-Sian; Chang, Chun-Chi; Lin, Ching-Hsiung; Chang, Jan-Gowth

    2015-05-01

    Cytochrome P450 2D6 is one of the important enzymes involved in the metabolism of many widely used drugs. Genetic polymorphisms of CYP2D6 can affect its activity. Therefore, an efficient method for identifying CYP2D6 polymorphisms is clinically important. We developed a high-resolution melting (HRM) analysis to investigate CYP2D6 polymorphisms. Genomic DNA was extracted from peripheral blood samples from 71 healthy individuals. All nine exons of the CYP2D6 gene were sequenced before screening by HRM analysis. This method can detect the most genotypes (*1, *2, *4, *10, *14, *21 *39, and *41) of CYP2D6 in Chinese. All samples were successfully genotyped. The four most common mutant CYP2D6 alleles (*1, *2, *10, and *41) can be genotyped. The single nucleotides polymorphism (SNP) frequencies of 100C > T (rs1065852), 1039C > T (rs1081003), 1661G > C (rs1058164), 2663G > A (rs28371722), 2850C > T (rs16947), 2988G > A (rs28371725), 3181A > G, and 4180G > C (rs1135840) were 58%, 61%, 73%, 1%, 13%, 3%, 1%, 73%, respectively. We identified 100% of all heterozygotes without any errors. The two homozygous genotypes (1661G > C and 4180G > C) can be distinguished by mixing with a known genotype sample to generate an artificial heterozygote for HRM analysis. Therefore, all samples could be identified using our HRM method, and the results of HRM analysis are identical to those obtained by sequencing. Our method achieved 100% sensitivity, specificity, positive prediction value and negative prediction value. HRM analysis is a nongel resolution method that is faster and less expensive than direct sequencing. Our study shows that it is an efficient tool for typing CYP2D6 polymorphisms. © 2014 Wiley Periodicals, Inc.

  13. Alternative SNP detection platforms, HRM and biosensors, for varietal identification in Vitis vinifera L. using F3H and LDOX genes.

    Science.gov (United States)

    Gomes, Sónia; Castro, Cláudia; Barrias, Sara; Pereira, Leonor; Jorge, Pedro; Fernandes, José R; Martins-Lopes, Paula

    2018-04-11

    The wine sector requires quick and reliable methods for Vitis vinifera L. varietal identification. The number of V. vinifera varieties is estimated in about 5,000 worldwide. Single Nucleotide Polymorphisms (SNPs) represent the most basic and abundant form of genetic sequence variation, being adequate for varietal discrimination. The aim of this work was to develop DNA-based assays suitable to detect SNP variation in V. vinifera, allowing varietal discrimination. Genotyping by sequencing allowed the detection of eleven SNPs on two genes of the anthocyanin pathway, the flavanone 3-hydroxylase (F3H, EC: 1.14.11.9), and the leucoanthocyanidin dioxygenase (LDOX, EC 1.14.11.19; synonym anthocyanidin synthase, ANS) in twenty V. vinifera varieties. Three High Resolution Melting (HRM) assays were designed based on the sequencing information, discriminating five of the 20 varieties: Alicante Bouschet, Donzelinho Tinto, Merlot, Moscatel Galego and Tinta Roriz. Sanger sequencing of the HRM assay products confirmed the HRM profiles. Three probes, with different lengths and sequences, were used as bio-recognition elements in an optical biosensor platform based on a long period grating (LPG) fiber optic sensor. The label free platform detected a difference of a single SNP using genomic DNA samples. The two different platforms were successfully applied for grapevine varietal identification.

  14. Examining the link between HRM Practices and Employees’ performance in Nigerian public sector

    Directory of Open Access Journals (Sweden)

    Abubakar Tabiu

    2016-06-01

    Full Text Available Improving employee and organizational performance has been the main concern of many organizations for decades and several factors have also been studied as predictors of employee performance in organizations. However, studies that comprehensively measured all the dimensions of employee performance that enhance organizational effectiveness are limited. This paper explored the influence of HRM practices on the employee performance in the Nigerian public sector. Using cross sectional method on 265 participants from local government areas, the findings revealed that HRM practices such as job rotation, job autonomy and career planning had a significant and positive influence on all the three dimensions of employee performance (task, contextual and adaptive performance.

  15. Limited copy number-high resolution melting (LCN-HRM) enables the detection and identification by sequencing of low level mutations in cancer biopsies.

    Science.gov (United States)

    Do, Hongdo; Dobrovic, Alexander

    2009-10-08

    Mutation detection in clinical tumour samples is challenging when the proportion of tumour cells, and thus mutant alleles, is low. The limited sensitivity of conventional sequencing necessitates the adoption of more sensitive approaches. High resolution melting (HRM) is more sensitive than sequencing but identification of the mutation is desirable, particularly when it is important to discriminate false positives due to PCR errors or template degradation from true mutations.We thus developed limited copy number - high resolution melting (LCN-HRM) which applies limiting dilution to HRM. Multiple replicate reactions with a limited number of target sequences per reaction allow low level mutations to be detected. The dilutions used (based on Ct values) are chosen such that mutations, if present, can be detected by the direct sequencing of amplicons with aberrant melting patterns. Using cell lines heterozygous for mutations, we found that the mutations were not readily detected when they comprised 10% of total alleles (20% tumour cells) by sequencing, whereas they were readily detectable at 5% total alleles by standard HRM. LCN-HRM allowed these mutations to be identified by direct sequencing of those positive reactions.LCN-HRM was then used to review formalin-fixed paraffin-embedded (FFPE) clinical samples showing discordant findings between sequencing and HRM for KRAS exon 2 and EGFR exons 19 and 21. Both true mutations present at low levels and sequence changes due to artefacts were detected by LCN-HRM. The use of high fidelity polymerases showed that the majority of the artefacts were derived from the damaged template rather than replication errors during amplification. LCN-HRM bridges the sensitivity gap between HRM and sequencing and is effective in distinguishing between artefacts and true mutations.

  16. Applied Mathematical Methods in Theoretical Physics

    Science.gov (United States)

    Masujima, Michio

    2005-04-01

    All there is to know about functional analysis, integral equations and calculus of variations in a single volume. This advanced textbook is divided into two parts: The first on integral equations and the second on the calculus of variations. It begins with a short introduction to functional analysis, including a short review of complex analysis, before continuing a systematic discussion of different types of equations, such as Volterra integral equations, singular integral equations of Cauchy type, integral equations of the Fredholm type, with a special emphasis on Wiener-Hopf integral equations and Wiener-Hopf sum equations. After a few remarks on the historical development, the second part starts with an introduction to the calculus of variations and the relationship between integral equations and applications of the calculus of variations. It further covers applications of the calculus of variations developed in the second half of the 20th century in the fields of quantum mechanics, quantum statistical mechanics and quantum field theory. Throughout the book, the author presents over 150 problems and exercises -- many from such branches of physics as quantum mechanics, quantum statistical mechanics, and quantum field theory -- together with outlines of the solutions in each case. Detailed solutions are given, supplementing the materials discussed in the main text, allowing problems to be solved making direct use of the method illustrated. The original references are given for difficult problems. The result is complete coverage of the mathematical tools and techniques used by physicists and applied mathematicians Intended for senior undergraduates and first-year graduates in science and engineering, this is equally useful as a reference and self-study guide.

  17. Detection and quantification of Spirocerca lupi by HRM qPCR in fecal samples from dogs with spirocercosis.

    Science.gov (United States)

    Rojas, Alicia; Segev, Gilad; Markovics, Alex; Aroch, Itamar; Baneth, Gad

    2017-09-19

    Spirocerca lupi, the dog oesophageal nematode, causes a potentially fatal disease in domestic dogs, and is currently clinically diagnosed by coproscopy and oesophagoscopy. To date, a single molecular method, a semi-nested PCR, targeting the cox1 gene, has been developed to aid in the diagnosis of spirocercosis. The present study describes three novel high-resolution melt (HRM) quantitative PCR (qPCR) assays targeting fragments of the ITS1, 18S and cytb loci of S. lupi. The performance of these molecular assays in feces was compared to fecal flotation and to the previously described cox1 gene semi-nested PCR in 18 fecal samples from dogs with clinical oesophageal spirocercosis diagnosed by oesophagoscopy. The HRM qPCR for ITS1 and 18S were both able to detect 0.2 S. lupi eggs per gram (epg), while the HRM qPCR for the cytb and the semi-nested PCR for the cox1 detected 6 epg and 526 epg, respectively. Spirocerca lupi was detected in 61.1%, 44.4%, 27.8%, 11.1% and 5.6% of the fecal samples of dogs diagnosed with spirocercosis by using the ITS1 and 18S HRM qPCR assays, fecal flotation, cytb HRM qPCR and cox1 semi-nested PCR, respectively. All dogs positive by fecal flotation were also positive by ITS1 and 18S HRM qPCRs. Quantification of S. lupi eggs was successfully achieved in the HRM qPCRs and compared to the fecal flotation with no significant difference in the calculated concentrations between the HRM qPCRs that detected the 18S and ITS1 loci and the fecal flotation. The HRM qPCR for the 18S cross-amplified DNA from Toxocara canis and Toxascaris leonina. In contrast, the HRM qPCR for ITS1 did not cross-amplify DNA from other canine gastrointestinal parasites. This study presents two new molecular assays with significantly increased sensitivity for confirming and quantifying fecal S. lupi eggs. Of these, the HRM qPCR for ITS1 showed the best performance in terms of the limit of detection and absence of cross-amplification with other parasites. These assays will be

  18. The Transfer of HRM Policies and Practices in American Multinational Hotels in Saudi Arabia (P.155-164

    Directory of Open Access Journals (Sweden)

    Amal Hatem Alkhaldi

    2016-03-01

    Full Text Available Global   competition   in   the   international   business   environment has   pushed companies to achieve competitive advantage through mergers, acquisitions and through locating their subsidiaries in less developed counties for cost effectiveness. Consequently, the competitive pressure has increased the significance of human resource management (HRM in multinational companies (MNCs, and MNCs have recognised the significance of the transfer HRM process across borders. This study examines the transfer of HRM policies and practices of US MNCs to their subsidiaries in Saudi Arabia. The aim of the study is to determine the extent to which US MNCs transfer HRM policies and practices from their Headquarters to their subsidiaries in Saudi Arabia; and identify the factors that facilitate and inhibit HRM transfer. The paper is based on an investigation of the interaction between home-country and host-country effects in determining HRM policies and practices in MNCs in the context of the Middle East. The study adopts a mixed methods approach of documentary analysis, focus group interviews of employees and in depth interviews of key informants in four subsidiaries of US owned international hotel chains located in Riyadh, Saudi Arabia.Keywords: Home  country, host  country, human  resource  managem transfer,Institutional theory, culture conditions.

  19. Why Complementary HRM Practices Impact Performance

    DEFF Research Database (Denmark)

    Foss, Nicolai Juul; Pedersen, Torben; Reinholt, Mia

    2015-01-01

    the influence of single practices on individual-level knowledge sharing, but fails to include the influence of combinations of practices. We link the idea of fit between practices to employee motivation for knowledge sharing by arguing that rewards may be ambiguous and difficult to interpret...... in the form of a three-way interaction among these variables with respect to their impact on knowledge-sharing motivation. Our analysis of 1,523 employees in five knowledge-intensive firms shows that employees who are exposed to knowledge-sharing rewards experience higher levels of autonomous motivation......, but that such ambiguity may be reduced if rewards are combined with other aligned HRM practices, notably job design and work climate. Thus, fit is established through the ambiguity-reducing effect of combining specific HRM practices. Accordingly, we test for complementarities among rewards, job design, and work climate...

  20. High-resolution melting (HRM) assay for the detection of recurrent BRCA1/BRCA2 germline mutations in Tunisian breast/ovarian cancer families.

    Science.gov (United States)

    Riahi, Aouatef; Kharrat, Maher; Lariani, Imen; Chaabouni-Bouhamed, Habiba

    2014-12-01

    Germline deleterious mutations in the BRCA1/BRCA2 genes are associated with an increased risk for the development of breast and ovarian cancer. Given the large size of these genes the detection of such mutations represents a considerable technical challenge. Therefore, the development of cost-effective and rapid methods to identify these mutations became a necessity. High resolution melting analysis (HRM) is a rapid and efficient technique extensively employed as high-throughput mutation scanning method. The purpose of our study was to assess the specificity and sensitivity of HRM for BRCA1 and BRCA2 genes scanning. As a first step we estimate the ability of HRM for detection mutations in a set of 21 heterozygous samples harboring 8 different known BRCA1/BRCA2 variations, all samples had been preliminarily investigated by direct sequencing, and then we performed a blinded analysis by HRM in a set of 68 further sporadic samples of unknown genotype. All tested heterozygous BRCA1/BRCA2 variants were easily identified. However the HRM assay revealed further alteration that we initially had not searched (one unclassified variant). Furthermore, sequencing confirmed all the HRM detected mutations in the set of unknown samples, including homozygous changes, indicating that in this cohort, with the optimized assays, the mutations detections sensitivity and specificity were 100 %. HRM is a simple, rapid and efficient scanning method for known and unknown BRCA1/BRCA2 germline mutations. Consequently the method will allow for the economical screening of recurrent mutations in Tunisian population.

  1. HRM Practices and knowledge transfer in MNCS

    DEFF Research Database (Denmark)

    Minbaeva, Dana

    their        motivation. Temporary and permanent types of international assignments        respectively influence the ability and motivation of expatriate managers        to share their knowledge.Keywords: Human Resources; Personnel; Human        Resource Management; MultinationalCorporations; Organizational...... and        receivers.HRM practices relevant for absorptive capacity of subsidiary        employees form two groups - cognitive (job analysis, recruitment,        selection, international rotation, career management, training and        performance appraisal) and stimulative (promotion, performance......-based        compensation, internal transfer, orientation programs, job design and        flexible working practices). The application of cognitive HRM practices        enhances the ability of knowledge receivers to absorb transferred        knowledge, while the use of stimulative HRM practices increases...

  2. Organizational Structure and Firms' Demand for HRM Practices

    OpenAIRE

    Eriksson, Tor; Ortega, Jaime

    2014-01-01

    A question largely left unanswered in previous studies of firms’ use of HRM practices, and the consequences thereof, is why some firms adopt these practices while others do not. We examine empirically the determinants of firms’ demand for HRM pay, work and training practices with a special focus on the role of differences in the organizational structure of firms. For this purpose we merge data from a detailed questionnaire study of Danish private sector firms’ use of HRM practices with workfo...

  3. DNA barcoding coupled to HRM analysis as a new and simple tool for the authentication of Gadidae fish species.

    Science.gov (United States)

    Fernandes, Telmo J R; Costa, Joana; Oliveira, M Beatriz P P; Mafra, Isabel

    2017-09-01

    This work aimed to exploit the use of DNA mini-barcodes combined with high resolution melting (HRM) for the authentication of gadoid species: Atlantic cod (Gadus morhua), Pacific cod (Gadus macrocephalus), Alaska pollock (Theragra chalcogramma) and saithe (Pollachius virens). Two DNA barcode regions, namely cytochrome c oxidase subunit I (COI) and cytochrome b (cytb), were analysed in silico to identify genetic variability among the four species and used, subsequently, to develop a real-time PCR method coupled with HRM analysis. The cytb mini-barcode enabled best discrimination of the target species with a high level of confidence (99.3%). The approach was applied successfully to identify gadoid species in 30 fish-containing foods, 30% of which were not as declared on the label. Herein, a novel approach for rapid, simple and cost-effective discrimination/clustering, as a tool to authenticate Gadidae fish species, according to their genetic relationship, is proposed. Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. The HRM practices of innovative knowledge-intensive firms

    DEFF Research Database (Denmark)

    Jørgensen, Frances; Becker, Karen; Matthews, Judy

    2011-01-01

    , is the basis of this research. The study investigates the HRM practices being used by four innovative firms: two KIFs and two traditional production companies. The findings suggest that there are some similarities between the HRM practices of the case firms, but there are also important differences......There is little research on HRM practices in knowledge-intensive firms (KIFs), especially in comparison to that conducted in traditional production environments. Whether such HRM practices are appropriate for KIFs, particularly ones that differentiate themselves on their ability to innovate...

  5. Applying scrum methods to ITS projects.

    Science.gov (United States)

    2017-08-01

    The introduction of new technology generally brings new challenges and new methods to help with deployments. Agile methodologies have been introduced in the information technology industry to potentially speed up development. The Federal Highway Admi...

  6. Applying Fuzzy Possibilistic Methods on Critical Objects

    DEFF Research Database (Denmark)

    Yazdani, Hossein; Ortiz-Arroyo, Daniel; Choros, Kazimierz

    2016-01-01

    Providing a flexible environment to process data objects is a desirable goal of machine learning algorithms. In fuzzy and possibilistic methods, the relevance of data objects is evaluated and a membership degree is assigned. However, some critical objects objects have the potential ability to affect...... the performance of the clustering algorithms if they remain in a specific cluster or they are moved into another. In this paper we analyze and compare how critical objects affect the behaviour of fuzzy possibilistic methods in several data sets. The comparison is based on the accuracy and ability of learning...... methods to provide a proper searching space for data objects. The membership functions used by each method when dealing with critical objects is also evaluated. Our results show that relaxing the conditions of participation for data objects in as many partitions as they can, is beneficial....

  7. Use of a high resolution melting (HRM assay to compare gag, pol, and env diversity in adults with different stages of HIV infection.

    Directory of Open Access Journals (Sweden)

    Matthew M Cousins

    Full Text Available Cross-sectional assessment of HIV incidence relies on laboratory methods to discriminate between recent and non-recent HIV infection. Because HIV diversifies over time in infected individuals, HIV diversity may serve as a biomarker for assessing HIV incidence. We used a high resolution melting (HRM diversity assay to compare HIV diversity in adults with different stages of HIV infection. This assay provides a single numeric HRM score that reflects the level of genetic diversity of HIV in a sample from an infected individual.HIV diversity was measured in 203 adults: 20 with acute HIV infection (RNA positive, antibody negative, 116 with recent HIV infection (tested a median of 189 days after a previous negative HIV test, range 14-540 days, and 67 with non-recent HIV infection (HIV infected >2 years. HRM scores were generated for two regions in gag, one region in pol, and three regions in env.Median HRM scores were higher in non-recent infection than in recent infection for all six regions tested. In multivariate models, higher HRM scores in three of the six regions were independently associated with non-recent HIV infection.The HRM diversity assay provides a simple, scalable method for measuring HIV diversity. HRM scores, which reflect the genetic diversity in a viral population, may be useful biomarkers for evaluation of HIV incidence, particularly if multiple regions of the HIV genome are examined.

  8. Use of a high resolution melting (HRM) assay to compare gag, pol, and env diversity in adults with different stages of HIV infection.

    Science.gov (United States)

    Cousins, Matthew M; Laeyendecker, Oliver; Beauchamp, Geetha; Brookmeyer, Ronald; Towler, William I; Hudelson, Sarah E; Khaki, Leila; Koblin, Beryl; Chesney, Margaret; Moore, Richard D; Kelen, Gabor D; Coates, Thomas; Celum, Connie; Buchbinder, Susan P; Seage, George R; Quinn, Thomas C; Donnell, Deborah; Eshleman, Susan H

    2011-01-01

    Cross-sectional assessment of HIV incidence relies on laboratory methods to discriminate between recent and non-recent HIV infection. Because HIV diversifies over time in infected individuals, HIV diversity may serve as a biomarker for assessing HIV incidence. We used a high resolution melting (HRM) diversity assay to compare HIV diversity in adults with different stages of HIV infection. This assay provides a single numeric HRM score that reflects the level of genetic diversity of HIV in a sample from an infected individual. HIV diversity was measured in 203 adults: 20 with acute HIV infection (RNA positive, antibody negative), 116 with recent HIV infection (tested a median of 189 days after a previous negative HIV test, range 14-540 days), and 67 with non-recent HIV infection (HIV infected >2 years). HRM scores were generated for two regions in gag, one region in pol, and three regions in env. Median HRM scores were higher in non-recent infection than in recent infection for all six regions tested. In multivariate models, higher HRM scores in three of the six regions were independently associated with non-recent HIV infection. The HRM diversity assay provides a simple, scalable method for measuring HIV diversity. HRM scores, which reflect the genetic diversity in a viral population, may be useful biomarkers for evaluation of HIV incidence, particularly if multiple regions of the HIV genome are examined.

  9. Quality assurance and applied statistics. Method 3

    International Nuclear Information System (INIS)

    1992-01-01

    This German-Industry-Standards-paperback contains the International Standards from the Series ISO 9000 (or, as the case may be, the European Standards from the Series EN 29000) concerning quality assurance and including the already completed supplementary guidelines with ISO 9000- and ISO 9004-section numbers, which have been adopted as German Industry Standards and which are observed and applied world-wide to a great extent. It also includes the German-Industry-Standards ISO 10011 parts 1, 2 and 3 concerning the auditing of quality-assurance systems and the German-Industry-Standard ISO 10012 part 1 concerning quality-assurance demands (confirmation system) for measuring devices. The standards also include English and French versions. They are applicable independent of the user's line of industry and thus constitute basic standards. (orig.) [de

  10. Lavine method applied to three body problems

    International Nuclear Information System (INIS)

    Mourre, Eric.

    1975-09-01

    The methods presently proposed for the three body problem in quantum mechanics, using the Faddeev approach for proving the asymptotic completeness, come up against the presence of new singularities when the potentials considered v(α)(x(α)) for two-particle interactions decay less rapidly than /x(α)/ -2 ; and also when trials are made for solving the problem with a representation space whose dimension for a particle is lower than three. A method is given that allows the mathematical approach to be extended to three body problem, in spite of singularities. Applications are given [fr

  11. Applying Human Computation Methods to Information Science

    Science.gov (United States)

    Harris, Christopher Glenn

    2013-01-01

    Human Computation methods such as crowdsourcing and games with a purpose (GWAP) have each recently drawn considerable attention for their ability to synergize the strengths of people and technology to accomplish tasks that are challenging for either to do well alone. Despite this increased attention, much of this transformation has been focused on…

  12. Applying Mixed Methods Techniques in Strategic Planning

    Science.gov (United States)

    Voorhees, Richard A.

    2008-01-01

    In its most basic form, strategic planning is a process of anticipating change, identifying new opportunities, and executing strategy. The use of mixed methods, blending quantitative and qualitative analytical techniques and data, in the process of assembling a strategic plan can help to ensure a successful outcome. In this article, the author…

  13. [The diagnostic methods applied in mycology].

    Science.gov (United States)

    Kurnatowska, Alicja; Kurnatowski, Piotr

    2008-01-01

    The systemic fungal invasions are recognized with increasing frequency and constitute a primary cause of morbidity and mortality, especially in immunocompromised patients. Early diagnosis improves prognosis, but remains a problem because there is lack of sensitive tests to aid in the diagnosis of systemic mycoses on the one hand, and on the other the patients only present unspecific signs and symptoms, thus delaying early diagnosis. The diagnosis depends upon a combination of clinical observation and laboratory investigation. The successful laboratory diagnosis of fungal infection depends in major part on the collection of appropriate clinical specimens for investigations and on the selection of appropriate microbiological test procedures. So these problems (collection of specimens, direct techniques, staining methods, cultures on different media and non-culture-based methods) are presented in article.

  14. Monte Carlo method applied to medical physics

    International Nuclear Information System (INIS)

    Oliveira, C.; Goncalves, I.F.; Chaves, A.; Lopes, M.C.; Teixeira, N.; Matos, B.; Goncalves, I.C.; Ramalho, A.; Salgado, J.

    2000-01-01

    The main application of the Monte Carlo method to medical physics is dose calculation. This paper shows some results of two dose calculation studies and two other different applications: optimisation of neutron field for Boron Neutron Capture Therapy and optimization of a filter for a beam tube for several purposes. The time necessary for Monte Carlo calculations - the highest boundary for its intensive utilisation - is being over-passed with faster and cheaper computers. (author)

  15. Proteomics methods applied to malaria: Plasmodium falciparum

    International Nuclear Information System (INIS)

    Cuesta Astroz, Yesid; Segura Latorre, Cesar

    2012-01-01

    Malaria is a parasitic disease that has a high impact on public health in developing countries. The sequencing of the plasmodium falciparum genome and the development of proteomics have enabled a breakthrough in understanding the biology of the parasite. Proteomics have allowed to characterize qualitatively and quantitatively the parasite s expression of proteins and has provided information on protein expression under conditions of stress induced by antimalarial. Given the complexity of their life cycle, this takes place in the vertebrate host and mosquito vector. It has proven difficult to characterize the protein expression during each stage throughout the infection process in order to determine the proteome that mediates several metabolic, physiological and energetic processes. Two dimensional electrophoresis, liquid chromatography and mass spectrometry have been useful to assess the effects of antimalarial on parasite protein expression and to characterize the proteomic profile of different p. falciparum stages and organelles. The purpose of this review is to present state of the art tools and advances in proteomics applied to the study of malaria, and to present different experimental strategies used to study the parasite's proteome in order to show the advantages and disadvantages of each one.

  16. METHOD OF APPLYING NICKEL COATINGS ON URANIUM

    Science.gov (United States)

    Gray, A.G.

    1959-07-14

    A method is presented for protectively coating uranium which comprises etching the uranium in an aqueous etching solution containing chloride ions, electroplating a coating of nickel on the etched uranium and heating the nickel plated uranium by immersion thereof in a molten bath composed of a material selected from the group consisting of sodium chloride, potassium chloride, lithium chloride, and mixtures thereof, maintained at a temperature of between 700 and 800 deg C, for a time sufficient to alloy the nickel and uranium and form an integral protective coating of corrosion-resistant uranium-nickel alloy.

  17. Versatile Formal Methods Applied to Quantum Information.

    Energy Technology Data Exchange (ETDEWEB)

    Witzel, Wayne [Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States); Rudinger, Kenneth Michael [Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States); Sarovar, Mohan [Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)

    2015-11-01

    Using a novel formal methods approach, we have generated computer-veri ed proofs of major theorems pertinent to the quantum phase estimation algorithm. This was accomplished using our Prove-It software package in Python. While many formal methods tools are available, their practical utility is limited. Translating a problem of interest into these systems and working through the steps of a proof is an art form that requires much expertise. One must surrender to the preferences and restrictions of the tool regarding how mathematical notions are expressed and what deductions are allowed. Automation is a major driver that forces restrictions. Our focus, on the other hand, is to produce a tool that allows users the ability to con rm proofs that are essentially known already. This goal is valuable in itself. We demonstrate the viability of our approach that allows the user great exibility in expressing state- ments and composing derivations. There were no major obstacles in following a textbook proof of the quantum phase estimation algorithm. There were tedious details of algebraic manipulations that we needed to implement (and a few that we did not have time to enter into our system) and some basic components that we needed to rethink, but there were no serious roadblocks. In the process, we made a number of convenient additions to our Prove-It package that will make certain algebraic manipulations easier to perform in the future. In fact, our intent is for our system to build upon itself in this manner.

  18. Optimization methods applied to hybrid vehicle design

    Science.gov (United States)

    Donoghue, J. F.; Burghart, J. H.

    1983-01-01

    The use of optimization methods as an effective design tool in the design of hybrid vehicle propulsion systems is demonstrated. Optimization techniques were used to select values for three design parameters (battery weight, heat engine power rating and power split between the two on-board energy sources) such that various measures of vehicle performance (acquisition cost, life cycle cost and petroleum consumption) were optimized. The apporach produced designs which were often significant improvements over hybrid designs already reported on in the literature. The principal conclusions are as follows. First, it was found that the strategy used to split the required power between the two on-board energy sources can have a significant effect on life cycle cost and petroleum consumption. Second, the optimization program should be constructed so that performance measures and design variables can be easily changed. Third, the vehicle simulation program has a significant effect on the computer run time of the overall optimization program; run time can be significantly reduced by proper design of the types of trips the vehicle takes in a one year period. Fourth, care must be taken in designing the cost and constraint expressions which are used in the optimization so that they are relatively smooth functions of the design variables. Fifth, proper handling of constraints on battery weight and heat engine rating, variables which must be large enough to meet power demands, is particularly important for the success of an optimization study. Finally, the principal conclusion is that optimization methods provide a practical tool for carrying out the design of a hybrid vehicle propulsion system.

  19. Applying the Socratic Method to Physics Education

    Science.gov (United States)

    Corcoran, Ed

    2005-04-01

    We have restructured University Physics I and II in accordance with methods that PER has shown to be effective, including a more interactive discussion- and activity-based curriculum based on the premise that developing understanding requires an interactive process in which students have the opportunity to talk through and think through ideas with both other students and the teacher. Studies have shown that in classes implementing this approach to teaching as compared to classes using a traditional approach, students have significantly higher gains on the Force Concept Inventory (FCI). This has been true in UPI. However, UPI FCI results seem to suggest that there is a significant conceptual hole in students' understanding of Newton's Second Law. Two labs in UPI which teach Newton's Second Law will be redesigned replacing more activity with students as a group talking through, thinking through, and answering conceptual questions asked by the TA. The results will be measured by comparing FCI results to those from previous semesters, coupled with interviews. The results will be analyzed, and we will attempt to understand why gains were or were not made.

  20. Scanning probe methods applied to molecular electronics

    Energy Technology Data Exchange (ETDEWEB)

    Pavlicek, Niko

    2013-08-01

    Scanning probe methods on insulating films offer a rich toolbox to study electronic, structural and spin properties of individual molecules. This work discusses three issues in the field of molecular and organic electronics. An STM head to be operated in high magnetic fields has been designed and built up. The STM head is very compact and rigid relying on a robust coarse approach mechanism. This will facilitate investigations of the spin properties of individual molecules in the future. Combined STM/AFM studies revealed a reversible molecular switch based on two stable configurations of DBTH molecules on ultrathin NaCl films. AFM experiments visualize the molecular structure in both states. Our experiments allowed to unambiguously determine the pathway of the switch. Finally, tunneling into and out of the frontier molecular orbitals of pentacene molecules has been investigated on different insulating films. These experiments show that the local symmetry of initial and final electron wave function are decisive for the ratio between elastic and vibration-assisted tunneling. The results can be generalized to electron transport in organic materials.

  1. Er HRM andet end en sten i skoen?

    DEFF Research Database (Denmark)

    Holt Larsen, Henrik

    2015-01-01

    Ledelse. Arbejdet med virksomheders menneskelige ressourcer kræver, at ledelsen tror på det. Ellers kan det gå ud over HRM-indsatsen.......Ledelse. Arbejdet med virksomheders menneskelige ressourcer kræver, at ledelsen tror på det. Ellers kan det gå ud over HRM-indsatsen....

  2. HRM and innovation: Themes, contingencies and directions for future research

    NARCIS (Netherlands)

    Florén, H.; Rundquist, J.; Schuler, R.S.; Bondarouk, Tatiana; Ruel, Hubertus Johannes Maria

    2014-01-01

    The purposes of this special issue were to connect Human Resource Management (HRM) research and innovation research and to contribute towards a better understanding of how HRM can be deployed to support organisations in their innovation efforts. In this commentary, we review the results from the

  3. Off the mark : Response to Kaufman's evolution of strategic HRM

    NARCIS (Netherlands)

    Wright, Patrick M.; Guest, David; Paauwe, Jaap

    2015-01-01

    Professor Bruce Kaufman's look back at two seminal books published at the beginning of the strategic HRM field and examination of two recent books to trace the evolution of the field identifies some issues, but ones with which the field has dealt for a number of years. His choice of our book HRM and

  4. Scratching below the surface to uncover HRM practices in Denmark

    DEFF Research Database (Denmark)

    Jørgensen, Frances; Ulhøi, John Parm

    2010-01-01

    Is HRM in Denmark behind the times and only now beginning to be prioritized by Danish firms? Or is it possible that managers are successfully capitalizing on characteristics of the Danish society and the Danish workforce? We propose quite adamantly that HRM practices indeed are alive in Danish...

  5. Pride, prejudice and performance: relations between diversity, HRM and performance

    NARCIS (Netherlands)

    Benschop, Y.W.M.

    2001-01-01

    Today, managing diversity is considered one of the main challenges for HRM in modern organizations. In this article, a framework is developed to suggest that the design and implementation of HRM activities should be influenced by strategic choices on the management of diversity which are themselves

  6. HRM, climate and employee well-being : comparing an optimistic and critical perspective

    NARCIS (Netherlands)

    Veld, M.F.A.|info:eu-repo/dai/nl/341254282; Alfes, Kerstin

    2017-01-01

    A growing number of studies investigating the linkage between HRM and employee well-being largely supports a positive perspective on HRM (HRM positively affects employee well-being). There is only a limited body of empirical evidence that supports a critical perspective (HRM negatively affects

  7. E-HRM effectiveness in a Dutch ministry : results of survey and discursive exploration combined

    NARCIS (Netherlands)

    Bondarouk, Tanya; Ruel, Huub

    2006-01-01

    E-HRM is coming to a more full-grown stage within organisational life. Much is assumed and expressed about the advantages of e-HRM, however scientific proof of these advantages is scarce. No clarity exists about the answer to the question whether e-HRM contributes to HRM effectiveness. In this paper

  8. eHRM adoption in emerging economies: The case of subsidiaries of multinational corporations in Indonesia.

    NARCIS (Netherlands)

    Bondarouk, Tatiana; Schilling, Dustin; Ruel, Hubertus Johannes Maria

    2016-01-01

    Electronic HRM (eHRM) is assumed to strengthen the position of HRM as a business partner by promising strategic benefits. Empirical support for this assumption, however, mostly comes from studies conducted in developed economies. Yet eHRM adoption in the emerging economy context remains poorly

  9. The Signalling Effect of HRM on Psychological Contracts of Employees: A Multi-Level Perspective

    NARCIS (Netherlands)

    M. Sonnenberg

    2006-01-01

    textabstractWat bepaalt de effectiviteit van HRM? Zowel in wetenschappelijk HRM onderzoek als in de HRM praktijk is de relatie tussen HRM en organisatie prestaties de afgelopen jaren uitgegroeid tot het belangrijkste aandachtsgebied. Centraal in deze relatie staat de verhouding tussen werknemers en

  10. Reflections on Mixing Methods in Applied Linguistics Research

    Science.gov (United States)

    Hashemi, Mohammad R.

    2012-01-01

    This commentary advocates the use of mixed methods research--that is the integration of qualitative and quantitative methods in a single study--in applied linguistics. Based on preliminary findings from a research project in progress, some reflections on the current practice of mixing methods as a new trend in applied linguistics are put forward.…

  11. E-HRM effectiveness in a Dutch ministry: results of survey and discursive exploration combined

    OpenAIRE

    Bondarouk, Tanya; Ruel, Huub

    2006-01-01

    E-HRM is coming to a more full-grown stage within organisational life. Much is assumed and expressed about the advantages of e-HRM, however scientific proof of these advantages is scarce. No clarity exists about the answer to the question whether e-HRM contributes to HRM effectiveness. In this paper we present results from the first quantitative study on the contribution of e-HRM to HRM effectiveness. The data is collected in a Dutch ministry. Results show that actual use of the e-HRM applica...

  12. Applying homotopy analysis method for solving differential-difference equation

    International Nuclear Information System (INIS)

    Wang Zhen; Zou Li; Zhang Hongqing

    2007-01-01

    In this Letter, we apply the homotopy analysis method to solving the differential-difference equations. A simple but typical example is applied to illustrate the validity and the great potential of the generalized homotopy analysis method in solving differential-difference equation. Comparisons are made between the results of the proposed method and exact solutions. The results show that the homotopy analysis method is an attractive method in solving the differential-difference equations

  13. HRM in private and public organizations in Serbia

    Directory of Open Access Journals (Sweden)

    Berber Nemanja

    2016-01-01

    Full Text Available Human resource management (HRM is a management concept which obtains many practices and activities. Although there is a substantial literature on HRM in the private sector, the practice of HRM in the public sector is still scarce. In the private sector, HRM is found as a factor for gaining competitive advantage, especially if HR practices are implemented in the way of high-performing working practice (HPWP. In the public sector, HRM is seen as paternalistic management, with the standardization of employment practices, collective bargaining and working practices that emphasize equal opportunities for employees. The goal of this research is to explore the characteristics and differences between HRM practice in organizations from the private and public sector. The subject of the research is HRM practice (staffing, training and development, compensation and benefits, and industrial relation and communication in the private and public organizations in the Republic of Serbia. The methodology of the paper includes exploration of the available literature on the theme and statistical analysis of the differences between HR practices in organizations from the private and public sector. The research is based on the HR data gathered in the second CRANET research round in Serbia, performed in 2015.

  14. MGMT promoter methylation determined by HRM in comparison to MSP and pyrosequencing for predicting high-grade glioma response.

    Science.gov (United States)

    Switzeny, Olivier J; Christmann, Markus; Renovanz, Mirjam; Giese, Alf; Sommer, Clemens; Kaina, Bernd

    2016-01-01

    The DNA repair protein O(6)-methylguanine-DNA methyltransferase (MGMT) causes resistance of cancer cells to alkylating agents and, therefore, is a well-established predictive marker for high-grade gliomas that are routinely treated with alkylating drugs. Since MGMT is highly epigenetically regulated, the MGMT promoter methylation status is taken as an indicator of MGMT silencing, predicting the outcome of glioma therapy. MGMT promoter methylation is usually determined by methylation specific PCR (MSP), which is a labor intensive and error-prone method often used semi-quantitatively. Searching for alternatives, we used closed-tube high resolution melt (HRM) analysis, which is a quantitative method, and compared it with MSP and pyrosequencing regarding its predictive value. We analyzed glioblastoma cell lines with known MGMT activity and formalin-fixed samples from IDH1 wild-type high-grade glioma patients (WHO grade III/IV) treated with radiation and temozolomide by HRM, MSP, and pyrosequencing. The data were compared as to progression-free survival (PFS) and overall survival (OS) of patients exhibiting the methylated and unmethylated MGMT status. A promoter methylation cut-off level relevant for PFS and OS was determined. In a multivariate Cox regression model, methylation of MGMT promoter of high-grade gliomas analyzed by HRM, but not MSP, was found to be an independent predictive marker for OS. Univariate Kaplan-Meier analyses revealed for PFS and OS a significant and better discrimination between methylated and unmethylated tumors when quantitative HRM was used instead of MSP. Compared to MSP and pyrosequencing, the HRM method is simple, cost effective, highly accurate and fast. HRM is at least equivalent to pyrosequencing in quantifying the methylation level. It is superior in predicting PFS and OS of high-grade glioma patients compared to MSP and, therefore, can be recommended being used routinely for determination of the MGMT status of gliomas.

  15. The Effect of Blending HRM Transformational Leadership Style with HRM ICT Expertise Leadership Style on Creating New HRM Strategy That Enable National Companies to Go Global. Evidence from Jordan: Sayegh Group and Hikma Pharmaceutical Corporation

    Science.gov (United States)

    Khudeir, Hamzeh

    2016-01-01

    There is no single HRM strategy that can cure all HRM problems and complicated issues. Thus, most companies go by the book in this regard; however, the majorities have their own policies and strategies they use to achieve their objectives in general and competitive advantage in particular. One of the strategies used in HRM is blending HRM…

  16. Identification of Brucella spp. isolated from human brucellosis in Malaysia using high-resolution melt (HRM) analysis.

    Science.gov (United States)

    Mohamed Zahidi, Jama'ayah; Bee Yong, Tay; Hashim, Rohaidah; Mohd Noor, Azura; Hamzah, Siti Hawa; Ahmad, Norazah

    2015-04-01

    Molecular approaches have been investigated to overcome difficulties in identification and differentiation of Brucella spp. using conventional phenotypic methods. In this study, high-resolution melt (HRM) analysis was used for rapid identification and differentiation of members of Brucella genus. A total of 41 Brucella spp. isolates from human brucellosis were subjected to HRM analysis using 4 sets of primers, which identified 40 isolates as Brucella melitensis and 1 as Brucella canis. The technique utilized low DNA concentration and was highly reproducible. The assay is shown to be a useful diagnostic tool, which can rapidly differentiate Brucella up to species level. Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

  17. High Resolution Melting (HRM) analysis for mutation screening of RGSL1,RGS16 and RGS8 in breast cancer

    DEFF Research Database (Denmark)

    Wiechec, Emilia; Wiuf, Carsten Henrik; Overgaard, Jens

    2011-01-01

    coding exons of RGSL1, RGS16, and RGS8 in tumors from 200 breast cancer patients. All sequence variants detected by HRM resulted in abnormal shape of the melting curves. The identified mutations and known single nucleotide polymorphisms (SNP) were subsequently confirmed by sequencing, and distribution...... cancer patients. In addition, a total of seven known SNPs were identified in this study. Genotype distributions were not significantly different between breast cancer patients and controls. CONCLUSIONS AND IMPACT: Identification of novel mutations within RGSL1 provides a new insight...... into the pathophysiology of breast cancer. Moreover, the HRM analysis represents a reliable and highly sensitive method for mutation scanning of multiple exons....

  18. Bar-HRM for Authentication of Plant-Based Medicines: Evaluation of Three Medicinal Products Derived from Acanthaceae Species.

    Science.gov (United States)

    Osathanunkul, Maslin; Madesis, Panagiotis; de Boer, Hugo

    2015-01-01

    Medicinal plants are used as a popular alternative to synthetic drugs, both in developed and developing countries. The economic importance of the herbal and natural supplement industry is increasing every year. As the herbal industry grows, consumer safety is one issue that cannot be overlooked. Herbal products in Thai local markets are commonly sold without packaging or labels. Plant powders are stored in large bags or boxes, and therefore buying local herbal products poses a high risk of acquiring counterfeited, substituted and/or adulterated products. Due to these issues, a reliable method to authenticate products is needed. Here DNA barcoding was used in combination with High Resolution Melting analysis (Bar-HRM) to authenticate three medicinal Acanthaceae species (Acanthus ebracteatus, Andrographis paniculata and Rhinacanthus nasutus) commonly used in Thailand. The rbcL barcode was selected for use in primers design for HRM analysis to produce standard melting profiles of the selected species. Melting data from the HRM assay using the designed rbcL primers showed that the three chosen species could be distinguished from each other. HRM curves of all fifteen test samples indicated that three of tested products did not contain the indicated species. Two closely related species (A. paniculata and R. nasutus), which have a high level of morphological similarity, were interchanged with one another in three tested products. Incorrect information on packaging and labels of the tested herbal products was the cause of the results shown here. Morphological similarity among the species of interest also hindered the collection process. The Bar-HRM method developed here proved useful in aiding in the identification and authentication of herbal species in processed samples. In the future, species authentication through Bar-HRM could be used to promote consumer trust, as well as raising the quality of herbal products.

  19. Rapid Detection and Identification of Human Hookworm Infections through High Resolution Melting (HRM) Analysis

    Science.gov (United States)

    Ngui, Romano; Lim, Yvonne A. L.; Chua, Kek Heng

    2012-01-01

    Background Hookworm infections are still endemic in low and middle income tropical countries with greater impact on the socioeconomic and public health of the bottom billion of the world's poorest people. In this study, a real-time polymerase chain reaction (PCR) coupled with high resolution melting-curve (HRM) analysis was evaluated for an accurate, rapid and sensitive tool for species identification focusing on the five human hookworm species. Methods Real-time PCR coupled with HRM analysis targeting the second internal transcribed spacer (ITS-2) of nuclear ribosomal DNA as the genetic marker was used to identify and distinguish hookworm species in human samples. Unique and distinct characteristics of HRM patterns were produced for each of the five hookworm species. The melting curves were characterized by peaks of 79.24±0.05°C and 83.00±0.04°C for Necator americanus, 79.12±0.10°C for Ancylostoma duodenale, 79.40±0.10°C for Ancylostoma ceylanicum, 79.63±0.05°C for Ancylostoma caninum and 79.70±0.14°C for Ancylostoma braziliense. An evaluation of the method's sensitivity and specificity revealed that this assay was able to detect as low as 0.01 ng/µl hookworm DNA and amplification was only recorded for hookworm positive samples. Conclusion The HRM assay developed in this study is a rapid and straightforward method for the diagnosis, identification and discrimination of five human hookworms. This assay is simple compared to other probe-based genotyping methods as it does not require multiplexing, DNA sequencing or post-PCR processing. Therefore, this method offers a new alternative for rapid detection of human hookworm species. PMID:22844538

  20. A novel dNTP-limited PCR and HRM assay to detect Williams-Beuren syndrome.

    Science.gov (United States)

    Zhang, Lichen; Zhang, Xiaoqing; You, Guoling; Yu, Yongguo; Fu, Qihua

    2018-06-01

    Williams-Beuren syndrome (WBS) is caused by a microdeletion of chromosome arm 7q11.23. A rapid and inexpensive genotyping method to detect microdeletion on 7q11.23 needs to be developed for the diagnosis of WBS. This study describes the development of a new type of molecular diagnosis method to detect microdeletion on 7q11.23 based upon high-resolution melting (HRM). Four genes on 7q11.23 were selected as the target genes for the deletion genotyping. dNTP-limited duplex PCR was used to amplify the reference gene, CFTR, and one of the four genes respectively on 7q11.23. An HRM assay was performed on the PCR products, and the height ratio of the negative derivative peaks between the target gene and reference gene was employed to analyze the copy number variation of the target region. A new genotyping method for detecting 7q11.23 deletion was developed based upon dNTP-limited PCR and HRM, which cost only 96 min. Samples from 15 WBS patients and 12 healthy individuals were genotyped by this method in a blinded fashion, and the sensitivity and specificity was 100% (95% CI, 0.80-1, and 95% CI, 0.75-1, respectively) which was proved by CytoScan HD array. The HRM assay we developed is an rapid, inexpensive, and highly accurate method for genotyping 7q11.23 deletion. It is potentially useful in the clinical diagnosis of WBS. Copyright © 2018 Elsevier B.V. All rights reserved.

  1. Rapid detection and identification of human hookworm infections through high resolution melting (HRM analysis.

    Directory of Open Access Journals (Sweden)

    Romano Ngui

    Full Text Available BACKGROUND: Hookworm infections are still endemic in low and middle income tropical countries with greater impact on the socioeconomic and public health of the bottom billion of the world's poorest people. In this study, a real-time polymerase chain reaction (PCR coupled with high resolution melting-curve (HRM analysis was evaluated for an accurate, rapid and sensitive tool for species identification focusing on the five human hookworm species. METHODS: Real-time PCR coupled with HRM analysis targeting the second internal transcribed spacer (ITS-2 of nuclear ribosomal DNA as the genetic marker was used to identify and distinguish hookworm species in human samples. Unique and distinct characteristics of HRM patterns were produced for each of the five hookworm species. The melting curves were characterized by peaks of 79.24±0.05°C and 83.00±0.04°C for Necator americanus, 79.12±0.10°C for Ancylostoma duodenale, 79.40±0.10°C for Ancylostoma ceylanicum, 79.63±0.05°C for Ancylostoma caninum and 79.70±0.14°C for Ancylostoma braziliense. An evaluation of the method's sensitivity and specificity revealed that this assay was able to detect as low as 0.01 ng/µl hookworm DNA and amplification was only recorded for hookworm positive samples. CONCLUSION: The HRM assay developed in this study is a rapid and straightforward method for the diagnosis, identification and discrimination of five human hookworms. This assay is simple compared to other probe-based genotyping methods as it does not require multiplexing, DNA sequencing or post-PCR processing. Therefore, this method offers a new alternative for rapid detection of human hookworm species.

  2. Printing method and printer used for applying this method

    NARCIS (Netherlands)

    2006-01-01

    The invention pertains to a method for transferring ink to a receiving material using an inkjet printer having an ink chamber (10) with a nozzle (8) and an electromechanical transducer (16) in cooperative connection with the ink chamber, comprising actuating the transducer to generate a pressure

  3. Affecting innovation through HRM: the role of creative capital

    NARCIS (Netherlands)

    Veenendaal, Andre; van Velzen, Martijn; Looise, Jan C.

    2014-01-01

    This paper provides a new and integrated approach to understanding, through creative capital, the relationship between human resource management (HRM) and innovation. The conceptual framework presented, building on insights from social capital research and the field of regional economic development,

  4. High Resolution Melt (HRM) analysis is an efficient tool to genotype EMS mutants in complex crop genomes.

    Science.gov (United States)

    Lochlainn, Seosamh Ó; Amoah, Stephen; Graham, Neil S; Alamer, Khalid; Rios, Juan J; Kurup, Smita; Stoute, Andrew; Hammond, John P; Østergaard, Lars; King, Graham J; White, Phillip J; Broadley, Martin R

    2011-12-08

    Targeted Induced Loci Lesions IN Genomes (TILLING) is increasingly being used to generate and identify mutations in target genes of crop genomes. TILLING populations of several thousand lines have been generated in a number of crop species including Brassica rapa. Genetic analysis of mutants identified by TILLING requires an efficient, high-throughput and cost effective genotyping method to track the mutations through numerous generations. High resolution melt (HRM) analysis has been used in a number of systems to identify single nucleotide polymorphisms (SNPs) and insertion/deletions (IN/DELs) enabling the genotyping of different types of samples. HRM is ideally suited to high-throughput genotyping of multiple TILLING mutants in complex crop genomes. To date it has been used to identify mutants and genotype single mutations. The aim of this study was to determine if HRM can facilitate downstream analysis of multiple mutant lines identified by TILLING in order to characterise allelic series of EMS induced mutations in target genes across a number of generations in complex crop genomes. We demonstrate that HRM can be used to genotype allelic series of mutations in two genes, BraA.CAX1a and BraA.MET1.a in Brassica rapa. We analysed 12 mutations in BraA.CAX1.a and five in BraA.MET1.a over two generations including a back-cross to the wild-type. Using a commercially available HRM kit and the Lightscanner™ system we were able to detect mutations in heterozygous and homozygous states for both genes. Using HRM genotyping on TILLING derived mutants, it is possible to generate an allelic series of mutations within multiple target genes rapidly. Lines suitable for phenotypic analysis can be isolated approximately 8-9 months (3 generations) from receiving M3 seed of Brassica rapa from the RevGenUK TILLING service.

  5. Rapid and inexpensive body fluid identification by RNA profiling-based multiplex High Resolution Melt (HRM) analysis.

    Science.gov (United States)

    Hanson, Erin K; Ballantyne, Jack

    2013-01-01

    Positive identification of the nature of biological material present on evidentiary items can be crucial for understanding the circumstances surrounding a crime. However, traditional protein-based methods do not permit the identification of all body fluids and tissues, and thus molecular based strategies for the conclusive identification of all forensically relevant biological fluids and tissues need to be developed. Messenger RNA (mRNA) profiling is an example of such a molecular-based approach. Current mRNA body fluid identification assays involve capillary electrophoresis (CE) or quantitative RT-PCR (qRT-PCR) platforms, each with its own limitations. Both platforms require the use of expensive fluorescently labeled primers or probes. CE-based assays require separate amplification and detection steps thus increasing the analysis time. For qRT-PCR assays, only 3-4 markers can be included in a single reaction since each requires a different fluorescent dye. To simplify mRNA profiling assays, and reduce the time and cost of analysis, we have developed single- and multiplex body fluid High Resolution Melt (HRM) assays for the identification of common forensically relevant biological fluids and tissues. The incorporated biomarkers include IL19 (vaginal secretions), IL1F7 (skin), ALAS2 (blood), MMP10 (menstrual blood), HTN3 (saliva) and TGM4 (semen).  The HRM assays require only unlabeled PCR primers and a single saturating intercalating fluorescent dye (Eva Green). Each body-fluid-specific marker can easily be identified by the presence of a distinct melt peak. Usually, HRM assays are used to detect variants or isoforms for a single gene target. However, we have uniquely developed duplex and triplex HRM assays to permit the simultaneous detection of multiple targets per reaction. Here we describe the development and initial performance evaluation of the developed HRM assays. The results demonstrate the potential use of HRM assays for rapid, and relatively inexpensive

  6. High Resolution Melt (HRM analysis is an efficient tool to genotype EMS mutants in complex crop genomes

    Directory of Open Access Journals (Sweden)

    Lochlainn Seosamh Ó

    2011-12-01

    Full Text Available Abstract Background Targeted Induced Loci Lesions IN Genomes (TILLING is increasingly being used to generate and identify mutations in target genes of crop genomes. TILLING populations of several thousand lines have been generated in a number of crop species including Brassica rapa. Genetic analysis of mutants identified by TILLING requires an efficient, high-throughput and cost effective genotyping method to track the mutations through numerous generations. High resolution melt (HRM analysis has been used in a number of systems to identify single nucleotide polymorphisms (SNPs and insertion/deletions (IN/DELs enabling the genotyping of different types of samples. HRM is ideally suited to high-throughput genotyping of multiple TILLING mutants in complex crop genomes. To date it has been used to identify mutants and genotype single mutations. The aim of this study was to determine if HRM can facilitate downstream analysis of multiple mutant lines identified by TILLING in order to characterise allelic series of EMS induced mutations in target genes across a number of generations in complex crop genomes. Results We demonstrate that HRM can be used to genotype allelic series of mutations in two genes, BraA.CAX1a and BraA.MET1.a in Brassica rapa. We analysed 12 mutations in BraA.CAX1.a and five in BraA.MET1.a over two generations including a back-cross to the wild-type. Using a commercially available HRM kit and the Lightscanner™ system we were able to detect mutations in heterozygous and homozygous states for both genes. Conclusions Using HRM genotyping on TILLING derived mutants, it is possible to generate an allelic series of mutations within multiple target genes rapidly. Lines suitable for phenotypic analysis can be isolated approximately 8-9 months (3 generations from receiving M3 seed of Brassica rapa from the RevGenUK TILLING service.

  7. HRM, company performance and employee well-being

    OpenAIRE

    Vanhala, Sinikka; Tuomi, Kaija

    2006-01-01

    This paper is dealing with the relationships between HRM, company performance and employee well-being. The relationship between S/HRM and company performance has received much attention in prior literature, while the employee perspective has been widely neglected in this research tradition. The purpose of this paper is two-fold: first, to identify and evaluate how company performance and employee well-being are related, and, secondly, to evaluate the possibilities of HR policies and practices...

  8. Discrimination symbol applying method for sintered nuclear fuel product

    International Nuclear Information System (INIS)

    Ishizaki, Jin

    1998-01-01

    The present invention provides a symbol applying method for applying discrimination information such as an enrichment degree on the end face of a sintered nuclear product. Namely, discrimination symbols of information of powders are applied by a sintering aid to the end face of a molded member formed by molding nuclear fuel powders under pressure. Then, the molded product is sintered. The sintering aid comprises aluminum oxide, a mixture of aluminum oxide and silicon dioxide, aluminum hydride or aluminum stearate alone or in admixture. As an applying means of the sintering aid, discrimination symbols of information of powders are drawn by an isostearic acid on the end face of the molded product, and the sintering aid is sprayed thereto, or the sintering aid is applied directly, or the sintering aid is suspended in isostearic acid, and the suspension is applied with a brush. As a result, visible discrimination information can be applied to the sintered member easily. (N.H.)

  9. Building "Applied Linguistic Historiography": Rationale, Scope, and Methods

    Science.gov (United States)

    Smith, Richard

    2016-01-01

    In this article I argue for the establishment of "Applied Linguistic Historiography" (ALH), that is, a new domain of enquiry within applied linguistics involving a rigorous, scholarly, and self-reflexive approach to historical research. Considering issues of rationale, scope, and methods in turn, I provide reasons why ALH is needed and…

  10. Applying Mixed Methods Research at the Synthesis Level: An Overview

    Science.gov (United States)

    Heyvaert, Mieke; Maes, Bea; Onghena, Patrick

    2011-01-01

    Historically, qualitative and quantitative approaches have been applied relatively separately in synthesizing qualitative and quantitative evidence, respectively, in several research domains. However, mixed methods approaches are becoming increasingly popular nowadays, and practices of combining qualitative and quantitative research components at…

  11. Competitiveness and sustainability of HRM activities in Croatia –CRANET survey results

    OpenAIRE

    Nina Pološki Vokić

    2015-01-01

    The aim of the paper is to assess whether HRM practices in Croatia, as the newest European Union (EU) member country with only cca 20 years of free market economy which implies the change in the HRM philosophy, is heading towards more competitive or more sustainable HRM. In the theoretical part of the paper the characteristics of competitive HRM and sustainable HRM are provided. In the empirical part of the paper, using CRANET methodology, the survey of HRM practices in Croatia has been condu...

  12. Taxonomic Identification of Mediterranean Pines and Their Hybrids Based on the High Resolution Melting (HRM) and trnL Approaches: From Cytoplasmic Inheritance to Timber Tracing

    Science.gov (United States)

    Ganopoulos, Ioannis; Aravanopoulos, Filippos; Madesis, Panagiotis; Pasentsis, Konstantinos; Bosmali, Irene; Ouzounis, Christos; Tsaftaris, Athanasios

    2013-01-01

    Fast and accurate detection of plant species and their hybrids using molecular tools will facilitate the assessment and monitoring of local biodiversity in an era of climate and environmental change. Herein, we evaluate the utility of the plastid trnL marker for species identification applied to Mediterranean pines (Pinus spp.). Our results indicate that trnL is a very sensitive marker for delimiting species biodiversity. Furthermore, High Resolution Melting (HRM) analysis was exploited as a molecular fingerprint for fast and accurate discrimination of Pinus spp. DNA sequence variants. The trnL approach and the HRM analyses were extended to wood samples of two species (Pinus nigra and Pinus sylvestris) with excellent results, congruent to those obtained using leaf tissue. Both analyses demonstrate that hybrids from the P. brutia (maternal parent) × P. halepensis (paternal parent) cross, exhibit the P. halepensis profile, confirming paternal plastid inheritance in Group Halepensis pines. Our study indicates that a single one-step reaction method and DNA marker are sufficient for the identification of Mediterranean pines, their hybrids and the origin of pine wood. Furthermore, our results underline the potential for certain DNA regions to be used as novel biological information markers combined with existing morphological characters and suggest a relatively reliable and open taxonomic system that can link DNA variation to phenotype-based species or hybrid assignment status and direct taxa identification from recalcitrant tissues such as wood samples. PMID:23577179

  13. Rapid detection and identification of four major Schistosoma species by high-resolution melt (HRM) analysis.

    Science.gov (United States)

    Li, Juan; Zhao, Guang-Hui; Lin, RuiQing; Blair, David; Sugiyama, Hiromu; Zhu, Xing-Quan

    2015-11-01

    Schistosomiasis, caused by blood flukes belonging to several species of the genus Schistosoma, is a serious and widespread parasitic disease. Accurate and rapid differentiation of these etiological agents of animal and human schistosomiasis to species level can be difficult. We report a real-time PCR assay coupled with a high-resolution melt (HRM) assay targeting a portion of the nuclear 18S rDNA to detect, identify, and distinguish between four major blood fluke species (Schistosoma japonicum, Schistosoma mansoni, Schistosoma haematobium, and Schistosoma mekongi). Using this system, the Schistosoma spp. was accurately identified and could also be distinguished from all other trematode species with which they were compared. As little as 10(-5) ng genomic DNA from a Schistosoma sp. could be detected. This process is inexpensive, easy, and can be completed within 3 h. Examination of 21 representative Schistosoma samples from 15 geographical localities in seven endemic countries validated the value of the HRM detection assay and proved its reliability. The melting curves were characterized by peaks of 83.65 °C for S. japonicum and S. mekongi, 85.65 °C for S. mansoni, and 85.85 °C for S. haematobium. The present study developed a real-time PCR coupled with HRM analysis assay for detection and differential identification of S. mansoni, S. haematobium, S. japonicum, and S. mekongi. This method is rapid, sensitive, and inexpensive. It has important implications for epidemiological studies of Schistosoma.

  14. Discovery and validation of DNA hypomethylation biomarkers for liver cancer using HRM-specific probes.

    Directory of Open Access Journals (Sweden)

    Barbara Stefanska

    Full Text Available Poor prognosis of hepatocellular carcinoma (HCC associated with late diagnosis necessitates the development of early diagnostic biomarkers. We have previously delineated the landscape of DNA methylation in HCC patients unraveling the importance of promoter hypomethylation in activation of cancer- and metastasis-driving genes. The purpose of the present study was to test the feasibility that genes that are hypomethylated in HCC could serve as candidate diagnostic markers. We use high resolution melting analysis (HRM as a simple translatable PCR-based method to define methylation states in clinical samples. We tested seven regions selected from the shortlist of genes hypomethylated in HCC and showed that HRM analysis of several of them distinguishes methylation states in liver cancer specimens from normal adjacent liver and chronic hepatitis in the Shanghai area. Such regions were identified within promoters of neuronal membrane glycoprotein M6-B (GPM6B and melanoma antigen family A12 (MAGEA12 genes. Differences in HRM in the immunoglobulin superfamily Fc receptor (FCRL1 separated invasive tumors from less invasive HCC. The identified biomarkers differentiated HCC from chronic hepatitis in another set of samples from Dhaka. Although the main thrust in DNA methylation diagnostics in cancer is on hypermethylated genes, our study for the first time illustrates the potential use of hypomethylated genes as markers for solid tumors. After further validation in a larger cohort, the identified DNA hypomethylated regions can become important candidate biomarkers for liver cancer diagnosis and prognosis, especially in populations with high risk for HCC development.

  15. Candidate DNA Barcode Tags Combined With High Resolution Melting (Bar-HRM Curve Analysis for Authentication of Senna alexandrina Mill. With Validation in Crude Drugs

    Directory of Open Access Journals (Sweden)

    Priyanka Mishra

    2018-03-01

    Full Text Available Senna alexandrina (Fabaceae is a globally recognized medicinal plant for its laxative properties as well as the only source of sennosides, and is highly exported bulk herb from India. Its major procurement is exclusively from limited cultivation, which leads to risks of deliberate or unintended adulteration. The market raw materials are in powdered or finished product form, which lead to difficulties in authentication. Here, DNA barcode tags based on chloroplast genes (rbcL and matK and intergenic spacers (psbA-trnH and ITS were developed for S. alexandrina along with the allied species. The ability and performance of the ITS1 region to discriminate among the Senna species resulted in the present proposal of the ITS1 tags as successful barcode. Further, these tags were coupled with high-resolution melting (HRM curve analysis in a real-time PCR genotyping method to derive Bar-HRM (Barcoding-HRM assays. Suitable HRM primer sets were designed through SNP detection and mutation scanning in genomic signatures of Senna species. The melting profiles of S. alexandrina and S. italica subsp. micrantha were almost identical and the remaining five species were clearly separated so that they can be differentiated by HRM method. The sensitivity of the method was utilized to authenticate market samples [Herbal Sample Assays (HSAs]. HSA01 (S. alexandrina crude drug sample from Bangalore and HSA06 (S. alexandrina crude drug sample from Tuticorin, Tamil Nadu, India were found to be highly contaminated with S. italica subsp. micrantha. Species admixture samples mixed in varying percentage was identified sensitively with detection of contamination as low as 1%. The melting profiles of PCR amplicons are clearly distinct, which enables the authentic differentiation of species by the HRM method. This study reveals that DNA barcoding coupled with HRM is an efficient molecular tool to authenticate Senna herbal products in the market for quality control in the drug

  16. Candidate DNA Barcode Tags Combined With High Resolution Melting (Bar-HRM) Curve Analysis for Authentication of Senna alexandrina Mill. With Validation in Crude Drugs.

    Science.gov (United States)

    Mishra, Priyanka; Shukla, Ashutosh K; Sundaresan, Velusamy

    2018-01-01

    Senna alexandrina (Fabaceae) is a globally recognized medicinal plant for its laxative properties as well as the only source of sennosides, and is highly exported bulk herb from India. Its major procurement is exclusively from limited cultivation, which leads to risks of deliberate or unintended adulteration. The market raw materials are in powdered or finished product form, which lead to difficulties in authentication. Here, DNA barcode tags based on chloroplast genes ( rbcL and matK ) and intergenic spacers ( psbA-trnH and ITS ) were developed for S. alexandrina along with the allied species. The ability and performance of the ITS1 region to discriminate among the Senna species resulted in the present proposal of the ITS1 tags as successful barcode. Further, these tags were coupled with high-resolution melting (HRM) curve analysis in a real-time PCR genotyping method to derive Bar-HRM (Barcoding-HRM) assays. Suitable HRM primer sets were designed through SNP detection and mutation scanning in genomic signatures of Senna species. The melting profiles of S. alexandrina and S . italica subsp. micrantha were almost identical and the remaining five species were clearly separated so that they can be differentiated by HRM method. The sensitivity of the method was utilized to authenticate market samples [Herbal Sample Assays (HSAs)]. HSA01 ( S. alexandrina crude drug sample from Bangalore) and HSA06 ( S. alexandrina crude drug sample from Tuticorin, Tamil Nadu, India) were found to be highly contaminated with S . italica subsp. micrantha . Species admixture samples mixed in varying percentage was identified sensitively with detection of contamination as low as 1%. The melting profiles of PCR amplicons are clearly distinct, which enables the authentic differentiation of species by the HRM method. This study reveals that DNA barcoding coupled with HRM is an efficient molecular tool to authenticate Senna herbal products in the market for quality control in the drug supply

  17. Quantitative EEG Applying the Statistical Recognition Pattern Method

    DEFF Research Database (Denmark)

    Engedal, Knut; Snaedal, Jon; Hoegh, Peter

    2015-01-01

    BACKGROUND/AIM: The aim of this study was to examine the discriminatory power of quantitative EEG (qEEG) applying the statistical pattern recognition (SPR) method to separate Alzheimer's disease (AD) patients from elderly individuals without dementia and from other dementia patients. METHODS...

  18. High Resolution Melting (HRM) for High-Throughput Genotyping—Limitations and Caveats in Practical Case Studies

    Science.gov (United States)

    Słomka, Marcin; Sobalska-Kwapis, Marta; Wachulec, Monika; Bartosz, Grzegorz

    2017-01-01

    High resolution melting (HRM) is a convenient method for gene scanning as well as genotyping of individual and multiple single nucleotide polymorphisms (SNPs). This rapid, simple, closed-tube, homogenous, and cost-efficient approach has the capacity for high specificity and sensitivity, while allowing easy transition to high-throughput scale. In this paper, we provide examples from our laboratory practice of some problematic issues which can affect the performance and data analysis of HRM results, especially with regard to reference curve-based targeted genotyping. We present those examples in order of the typical experimental workflow, and discuss the crucial significance of the respective experimental errors and limitations for the quality and analysis of results. The experimental details which have a decisive impact on correct execution of a HRM genotyping experiment include type and quality of DNA source material, reproducibility of isolation method and template DNA preparation, primer and amplicon design, automation-derived preparation and pipetting inconsistencies, as well as physical limitations in melting curve distinction for alternative variants and careful selection of samples for validation by sequencing. We provide a case-by-case analysis and discussion of actual problems we encountered and solutions that should be taken into account by researchers newly attempting HRM genotyping, especially in a high-throughput setup. PMID:29099791

  19. High Resolution Melting (HRM) for High-Throughput Genotyping-Limitations and Caveats in Practical Case Studies.

    Science.gov (United States)

    Słomka, Marcin; Sobalska-Kwapis, Marta; Wachulec, Monika; Bartosz, Grzegorz; Strapagiel, Dominik

    2017-11-03

    High resolution melting (HRM) is a convenient method for gene scanning as well as genotyping of individual and multiple single nucleotide polymorphisms (SNPs). This rapid, simple, closed-tube, homogenous, and cost-efficient approach has the capacity for high specificity and sensitivity, while allowing easy transition to high-throughput scale. In this paper, we provide examples from our laboratory practice of some problematic issues which can affect the performance and data analysis of HRM results, especially with regard to reference curve-based targeted genotyping. We present those examples in order of the typical experimental workflow, and discuss the crucial significance of the respective experimental errors and limitations for the quality and analysis of results. The experimental details which have a decisive impact on correct execution of a HRM genotyping experiment include type and quality of DNA source material, reproducibility of isolation method and template DNA preparation, primer and amplicon design, automation-derived preparation and pipetting inconsistencies, as well as physical limitations in melting curve distinction for alternative variants and careful selection of samples for validation by sequencing. We provide a case-by-case analysis and discussion of actual problems we encountered and solutions that should be taken into account by researchers newly attempting HRM genotyping, especially in a high-throughput setup.

  20. Comparison Study of MS-HRM and Pyrosequencing Techniques for Quantification of APC and CDKN2A Gene Methylation

    Science.gov (United States)

    Migheli, Francesca; Stoccoro, Andrea; Coppedè, Fabio; Wan Omar, Wan Adnan; Failli, Alessandra; Consolini, Rita; Seccia, Massimo; Spisni, Roberto; Miccoli, Paolo; Mathers, John C.; Migliore, Lucia

    2013-01-01

    There is increasing interest in the development of cost-effective techniques for the quantification of DNA methylation biomarkers. We analyzed 90 samples of surgically resected colorectal cancer tissues for APC and CDKN2A promoter methylation using methylation sensitive-high resolution melting (MS-HRM) and pyrosequencing. MS-HRM is a less expensive technique compared with pyrosequencing but is usually more limited because it gives a range of methylation estimates rather than a single value. Here, we developed a method for deriving single estimates, rather than a range, of methylation using MS-HRM and compared the values obtained in this way with those obtained using the gold standard quantitative method of pyrosequencing. We derived an interpolation curve using standards of known methylated/unmethylated ratio (0%, 12.5%, 25%, 50%, 75%, and 100% of methylation) to obtain the best estimate of the extent of methylation for each of our samples. We observed similar profiles of methylation and a high correlation coefficient between the two techniques. Overall, our new approach allows MS-HRM to be used as a quantitative assay which provides results which are comparable with those obtained by pyrosequencing. PMID:23326336

  1. High Resolution Melting (HRM for High-Throughput Genotyping—Limitations and Caveats in Practical Case Studies

    Directory of Open Access Journals (Sweden)

    Marcin Słomka

    2017-11-01

    Full Text Available High resolution melting (HRM is a convenient method for gene scanning as well as genotyping of individual and multiple single nucleotide polymorphisms (SNPs. This rapid, simple, closed-tube, homogenous, and cost-efficient approach has the capacity for high specificity and sensitivity, while allowing easy transition to high-throughput scale. In this paper, we provide examples from our laboratory practice of some problematic issues which can affect the performance and data analysis of HRM results, especially with regard to reference curve-based targeted genotyping. We present those examples in order of the typical experimental workflow, and discuss the crucial significance of the respective experimental errors and limitations for the quality and analysis of results. The experimental details which have a decisive impact on correct execution of a HRM genotyping experiment include type and quality of DNA source material, reproducibility of isolation method and template DNA preparation, primer and amplicon design, automation-derived preparation and pipetting inconsistencies, as well as physical limitations in melting curve distinction for alternative variants and careful selection of samples for validation by sequencing. We provide a case-by-case analysis and discussion of actual problems we encountered and solutions that should be taken into account by researchers newly attempting HRM genotyping, especially in a high-throughput setup.

  2. HRM Challenges in the Age of Globalisation

    Directory of Open Access Journals (Sweden)

    Sugandha Agarwal

    2018-01-01

    Full Text Available Globalization has caused blurred international boundaries. On one hand, there is an open market for companies to seek talent on the other hand for employees a wide array of choices to work with, all because of globalization. But such types of open and globalized market exposes the firms to the challenges occurring in the prominent field of human resource. This paper is an attempt in understanding and analyzing the challenges existing and affecting the functioning of HRM while a company operates on global platform. The major encounters and worries of HR team, and the responses that HR managers need to make to such challenges with their increasing responsibilities in the globalized environment, have also been discussed in this paper. This is a communication and cross-sectional study based on descriptive arguments and analytical logic developed through the responses received from HR teams of companies belonging to different sectors. Effective leadership, managing diverse workforce, role of technology in HR functions like recruitment and selection, legal and political aspects, skill management, global mindset are some of the jostling issues encountered by human resource function in the retro of globalization.

  3. PENGEMBANGAN SISTEM HRM TERINTEGRASI DENGAN PENDEKATAN ERP

    Directory of Open Access Journals (Sweden)

    Christine Regilia Suwu

    2016-12-01

    Full Text Available Human Resources Management merupakan salah satu instrumen yang penting bagi perusahaan dalam pencapaian tujuannya. Penggunaan sistem informasi yang terintegrasi merupakan salah satu unsur penting untuk mengelola bisnis proses dalam manajemen perusahaan. ERP merupakan sistem informasi terintegrasi yang dimaksud dalam upaya peningkatan kualitas perusahaan. Khususnya untuk perusahaan distributor, pengelolaan proses bisnis secara konvensional dapat berpengaruh pada kualitas performa dari perusahaan itu sendiri. Berfokus pada HRM perusahaan distributor, penelitian membahas mengenai implementasi bisnis proses human resource management menggunakan software ERP yaitu Odoo. Penelitian dilakukan dengan menganalisa bisnis proses yang ada kemudian merancang sistem usulan yang terintegrasi lalu diimplementasikan ke dalam Odoo. Pemodelan sistem dirancang untuk mengoptimalkan pengelolaan data yang masih diproses secara konvensional. Optimalisasi dalam setiap proses bisnis meningkatkan kualitas keamanan dengan adanya pengaturan hak akses user dan keakuratan data yang didapat dengan transaksi data yang real time, serta efisiensi waktu yaitu memperkecil durasi waktu yang diperlukan untuk pencatatan dan penyimpanan dokumen dibanding secara manual. Kata Kunci: Sistem Informasi, Human Resources Management, ERP, Bussines Process Management

  4. Electronic-projecting Moire method applying CBR-technology

    Science.gov (United States)

    Kuzyakov, O. N.; Lapteva, U. V.; Andreeva, M. A.

    2018-01-01

    Electronic-projecting method based on Moire effect for examining surface topology is suggested. Conditions of forming Moire fringes and their parameters’ dependence on reference parameters of object and virtual grids are analyzed. Control system structure and decision-making subsystem are elaborated. Subsystem execution includes CBR-technology, based on applying case base. The approach related to analysing and forming decision for each separate local area with consequent formation of common topology map is applied.

  5. A Lagrangian meshfree method applied to linear and nonlinear elasticity.

    Science.gov (United States)

    Walker, Wade A

    2017-01-01

    The repeated replacement method (RRM) is a Lagrangian meshfree method which we have previously applied to the Euler equations for compressible fluid flow. In this paper we present new enhancements to RRM, and we apply the enhanced method to both linear and nonlinear elasticity. We compare the results of ten test problems to those of analytic solvers, to demonstrate that RRM can successfully simulate these elastic systems without many of the requirements of traditional numerical methods such as numerical derivatives, equation system solvers, or Riemann solvers. We also show the relationship between error and computational effort for RRM on these systems, and compare RRM to other methods to highlight its strengths and weaknesses. And to further explain the two elastic equations used in the paper, we demonstrate the mathematical procedure used to create Riemann and Sedov-Taylor solvers for them, and detail the numerical techniques needed to embody those solvers in code.

  6. Measuring the impact of HRM on organizational performance

    Directory of Open Access Journals (Sweden)

    Anastasia A. Katou

    2008-12-01

    Full Text Available Normal 0 21 false false false ES ZH-CN X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman","serif"; mso-ansi-language:ES-TRAD; mso-fareast-language:EN-US;} The purpose of this paper is to measure the impact of HRM on organisational performance in the context of Greece. Data were collected from 178 organisations using a questionnaire survey in the Greek manufacturing sector, and analysed using the ‘structural equation modelling’ methodology. The results indicated that the relationship between HRM policies (resourcing and development, compensation and incentives, involvement and job design and organisational performance is partially mediated through HRM outcomes (skills, attitudes, behaviour, and it is influenced by business strategies (cost, quality, innovation. Thus, the contribution of this study for academics and practitioners is that HRM policies associated with business strategies will affect organisational performance through HRM outcomes.

  7. Implementing Human Resources Management (HRM) within Dutch VET schools: Examining the fostering and hindering factors

    NARCIS (Netherlands)

    Runhaar, P.R.; Sanders, K.

    2013-01-01

    Vocational Education and Training (VET) Institutions face serious challenges, like the implementation of competence-based education and upcoming teacher shortages, which urge them to implement Human Resources Management policy and practices (HRM). The implementation of HRM, however, often stagnates.

  8. A high resolution melting (HRM) technology-based assay for cost-efficient clinical detection and genotyping of herpes simplex virus (HSV)-1 and HSV-2.

    Science.gov (United States)

    Lieveld, M; Carregosa, A; Benoy, I; Redzic, N; Berth, M; Vanden Broeck, D

    2017-10-01

    Genital herpes can be caused by two very similar viruses, herpes simplex virus (HSV)-1 or HSV-2. These two HSV types cannot be distinguished clinically, but genotyping is recommended in the first-episodes of genital herpes to guide counselling and management. Quantitative polymerase chain reaction (qPCR) is the preferred diagnostic method for HSV typing. However, commercial qPCR methods use expensive fluorescent labeled probes for detection. Furthermore, most low-cost methods are not able to differentiate between HSV-1 and -2. The aim of this study was to develop a high resolution melting (HRM) technology-based assay for sensitive HSV-1 and HSV-2 detection and genotyping. Using a panel of 46 clinical specimens, the performance of the HRM assay was compared to two commercial HSV tests: the HRM assay detected HSV in all 23 positive samples, with no false positive results (100% concordance with HSV I/II Real-TM assay). Additionally, the HRM assay correctly genotyped both HSV types in a subset of these clinical samples, as determined by the Realstar HSV PCR Kit. The HSV HRM assay provides a cost-effective alternative method to conventional more expensive assays and can be used in routine clinical specimens, in cases where it is particularly necessary to detect and distinguish HSV-1 from -2. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. E-HRM usage and value creation: Does a facilitating context matter?

    OpenAIRE

    Ruël, Huub; van der Kaap, Harry

    2012-01-01

    Electronic Human Resource Management (e-HRM) is assumed to be a driving force behind HRM value creation. However, the issue remains of whether empirical evidence supports this assumption. Moreover, is the relationship straightforward and direct or is it conditional, and do contextual factors intervene? This paper presents a study on this issue. Data was collected through a survey conducted in three international firms using e-HRM applications. The findings suggest that e-HRM usage in line wit...

  10. Comparison of allelic discrimination by dHPLC, HRM, and TaqMan in the detection of BRAF mutation V600E.

    Science.gov (United States)

    Carbonell, Pablo; Turpin, María C; Torres-Moreno, Daniel; Molina-Martínez, Irene; García-Solano, José; Perez-Guillermo, Miguel; Conesa-Zamora, Pablo

    2011-09-01

    The V600E mutation in the BRAF oncogene is associated with colorectal carcinomas, with mismatch-repair deficiency and, recently, with nonresponse to epidermal growth factor receptor inhibitor therapy. The use of reliable techniques for its detection is important. The aim of our study was to compare the performance characteristics in V600E detection of denaturing high-performance liquid chromatography (dHPLC) and high-resolution melting (HRM) with TaqMan allelic discrimination as well as direct-sequencing methods in a series of 195 colorectal paraffin-embedded specimens up to the age of 15 years. The effectiveness for obtaining results on mutation status was best using TaqMan (96.9%), followed by dHPLC (93.3%), HRM (88.7%), and sequencing (88.2%). In general, TaqMan was best for analyzing older tissues, whereas sequencing was the least efficient. Heterozygotic V600E was detected in 11.6%, 9.9%, 11.6%, and 9.9% of tissues using TaqMan, dHPLC, HRM, and sequencing, respectively. Result concordances between dHPLC and TaqMan or sequencing were excellent (κ = 0.9411 and κ = 0.8988, respectively); for HRM, the concordances were good (κ = 0.7973 and κ = 0.7488, respectively). By using DNA dilutions from tumor tissue, a minimum of 10% of V600E harboring cancer content was required for the analysis by dHPLC and HRM. dHPLC could detect four non-V600E mutations, whereas HRM detected one. Our results indicate that dHPLC and HRM are techniques that can be reliably used for the detection of the BRAFV600E mutation in archival paraffin-embedded tissues. Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.

  11. Applying the Taguchi method for optimized fabrication of bovine ...

    African Journals Online (AJOL)

    SERVER

    2008-02-19

    Feb 19, 2008 ... Nanobiotechnology Research Lab., School of Chemical Engineering, Babol University of Technology, Po.Box: 484, ... nanoparticle by applying the Taguchi method with characterization of the ... of BSA/ethanol and organic solvent adding rate. ... Sodium aside and all other chemicals were purchased from.

  12. Comparison of HRM practices between Chinese and Czech companies

    Directory of Open Access Journals (Sweden)

    Jun Li

    2016-03-01

    Full Text Available Both China and the Czech Republic have embarked on the transition from centrally planned economy to market-based economy since the early 1990s. Human resource management (HRM played a very important role in the success of companies’ economic transformation, and its role has been increasing since the period of stabilization and development of the market economy in both countries. Authors from both countries have been cooperating on the mutual comparative study on HRM practices in these two countries since the year 2009 until now and found out certain differences that were partially introduced in the doctoral thesis by Jun Li in 2011 after processing data from the first period of data collection. This paper briefly highlights some of the most obvious differences confirmed by the following mutual research. The areas where the HRM practices of both countries were compared more carefully are recruitment and selection process, training and development, performance appraisal and compensation.

  13. E-HRM effectiveness in a public sector organization: a multi-stakeholder perspective

    NARCIS (Netherlands)

    Bondarouk, Tatiana; Ruel, Hubertus Johannes Maria; van der Heijden, Beatrice

    2009-01-01

    Electronic HRM is increasingly gaining importance within working organizations and many of its adherents assume and express its advantages. Scientific support, however, is scarce and there is a lack of clarity regarding the contribution of e-HRM to HRM effectiveness. This article presents results

  14. The Process of Implementing HRM Practices : A Case Study in the Dutch Federal Government

    NARCIS (Netherlands)

    van Mierlo, Jorrit; Bondarouk, Tatiana

    2018-01-01

    Yearly, organisations create and implemented new HRM practices. To make those HRM practices work in an effective way, their implementation is of great importance. The goal of this paper is to uncover the mechanisms in which new HRM practices develop in federal governmental organisations. We do so by

  15. Is HRM a New Approach in the Employment Scene in Developing ...

    African Journals Online (AJOL)

    This paper attempts to discuss whether HRM represents a new approach to managing employment relationships in developing countries. It defines with brief descriptions the main key terminologies namely employment relationships, HRM and PM. The basic philosophy and values enshrined in the HRM model are explored.

  16. Framing the implementation of HRM innovation HR professionals vs line managers in a construction company

    NARCIS (Netherlands)

    Bondarouk, Tatiana; Looise, Jan C.; Lempsink, Bart

    2009-01-01

    Purpose – The purpose of this paper is to present the concept of human resource management (HRM) frames, to identify frame domains, and to explore their role in implementing HRM innovation. HRM innovation implementation is considered through the theoretical lens of social cognitive theory, and

  17. In-House vs. Off-the-Shelf e-HRM Applications

    NARCIS (Netherlands)

    Al-Ibraheem, Nawaf; Bondarouk, Tatiana; Ruel, Hubertus Johannes Maria; Guiderdoni-Jourdain, Karine; Oiry, Ewan

    2009-01-01

    Companies new to the e-HRM technologies are overwhelmed by the dilemma of choosing either the ready-made, off-the-shelf e-HRM systems, or develop their own e-HRM systems in house in order to implement the e-HR transformation. Therefore, this research was done to shed some light on the differences

  18. Quantitative PCR high-resolution melting (qPCR-HRM) curve analysis, a new approach to simultaneously screen point mutations and large rearrangements: application to MLH1 germline mutations in Lynch syndrome.

    Science.gov (United States)

    Rouleau, Etienne; Lefol, Cédrick; Bourdon, Violaine; Coulet, Florence; Noguchi, Tetsuro; Soubrier, Florent; Bièche, Ivan; Olschwang, Sylviane; Sobol, Hagay; Lidereau, Rosette

    2009-06-01

    Several techniques have been developed to screen mismatch repair (MMR) genes for deleterious mutations. Until now, two different techniques were required to screen for both point mutations and large rearrangements. For the first time, we propose a new approach, called "quantitative PCR (qPCR) high-resolution melting (HRM) curve analysis (qPCR-HRM)," which combines qPCR and HRM to obtain a rapid and cost-effective method suitable for testing a large series of samples. We designed PCR amplicons to scan the MLH1 gene using qPCR HRM. Seventy-six patients were fully scanned in replicate, including 14 wild-type patients and 62 patients with known mutations (57 point mutations and five rearrangements). To validate the detected mutations, we used sequencing and/or hybridization on a dedicated MLH1 array-comparative genomic hybridization (array-CGH). All point mutations and rearrangements detected by denaturing high-performance liquid chromatography (dHPLC)+multiplex ligation-dependent probe amplification (MLPA) were successfully detected by qPCR HRM. Three large rearrangements were characterized with the dedicated MLH1 array-CGH. One variant was detected with qPCR HRM in a wild-type patient and was located within the reverse primer. One variant was not detected with qPCR HRM or with dHPLC due to its proximity to a T-stretch. With qPCR HRM, prescreening for point mutations and large rearrangements are performed in one tube and in one step with a single machine, without the need for any automated sequencer in the prescreening process. In replicate, its reagent cost, sensitivity, and specificity are comparable to those of dHPLC+MLPA techniques. However, qPCR HRM outperformed the other techniques in terms of its rapidity and amount of data provided.

  19. Aircraft operability methods applied to space launch vehicles

    Science.gov (United States)

    Young, Douglas

    1997-01-01

    The commercial space launch market requirement for low vehicle operations costs necessitates the application of methods and technologies developed and proven for complex aircraft systems. The ``building in'' of reliability and maintainability, which is applied extensively in the aircraft industry, has yet to be applied to the maximum extent possible on launch vehicles. Use of vehicle system and structural health monitoring, automated ground systems and diagnostic design methods derived from aircraft applications support the goal of achieving low cost launch vehicle operations. Transforming these operability techniques to space applications where diagnostic effectiveness has significantly different metrics is critical to the success of future launch systems. These concepts will be discussed with reference to broad launch vehicle applicability. Lessons learned and techniques used in the adaptation of these methods will be outlined drawing from recent aircraft programs and implementation on phase 1 of the X-33/RLV technology development program.

  20. Magnetic stirring welding method applied to nuclear power plant

    International Nuclear Information System (INIS)

    Hirano, Kenji; Watando, Masayuki; Morishige, Norio; Enoo, Kazuhide; Yasuda, Yuuji

    2002-01-01

    In construction of a new nuclear power plant, carbon steel and stainless steel are used as base materials for the bottom linear plate of Reinforced Concrete Containment Vessel (RCCV) to achieve maintenance-free requirement, securing sufficient strength of structure. However, welding such different metals is difficult by ordinary method. To overcome the difficulty, the automated Magnetic Stirring Welding (MSW) method that can demonstrate good welding performance was studied for practical use, and weldability tests showed the good results. Based on the study, a new welding device for the MSW method was developed to apply it weld joints of different materials, and it practically used in part of a nuclear power plant. (author)

  1. Linear algebraic methods applied to intensity modulated radiation therapy.

    Science.gov (United States)

    Crooks, S M; Xing, L

    2001-10-01

    Methods of linear algebra are applied to the choice of beam weights for intensity modulated radiation therapy (IMRT). It is shown that the physical interpretation of the beam weights, target homogeneity and ratios of deposited energy can be given in terms of matrix equations and quadratic forms. The methodology of fitting using linear algebra as applied to IMRT is examined. Results are compared with IMRT plans that had been prepared using a commercially available IMRT treatment planning system and previously delivered to cancer patients.

  2. Rapid Identification of Echinococcus granulosus and E. canadensis Using High-Resolution Melting (HRM) Analysis by Focusing on a Single Nucleotide Polymorphism.

    Science.gov (United States)

    Safa, Ahmad Hosseini; Harandi, Majid Fasihi; Tajaddini, Mohammadhasan; Rostami-Nejad, Mohammad; Mohtashami-Pour, Mehdi; Pestehchian, Nader

    2016-07-22

    High-resolution melting (HRM) is a reliable and sensitive scanning method to detect variation in DNA sequences. We used this method to better understand the epidemiology and transmission of Echinococcus granulosus. We tested the use of HRM to discriminate the genotypes of E. granulosus and E. canadensis. One hundred forty-one hydatid cysts were collected from slaughtered animals in different parts of Isfahan-Iran in 2013. After DNA extraction, the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene was amplified using PCR coupled with the HRM curve. The result of HRM analysis using partial the sequences of cox1 gene revealed that 93, 35, and 2 isolates were identified as G1, G3, and G6 genotypes, respectively. A single nucleotide polymorphism (SNP) was found in locus 9867 of the cox1 gene. This is a critical locus for the differentiation between the G6 and G7 genotypes. In the phylogenic tree, the sample with a SNP was located between the G6 and G7 genotypes, which suggest that this isolate has a G6/G7 genotype. The HRM analysis developed in the present study provides a powerful technique for molecular and epidemiological studies on echinococcosis in humans and animals.

  3. MNC Knowledge Transfer, Subsidiary Absorptive Capacity and HRM

    DEFF Research Database (Denmark)

    Minbaeva, Dana; Pedersen, Torben; Björkman, Ingmar

    2014-01-01

    Based on a sample of 169 subsidiaries of multinational corporations (MNCs) operating in the USA, Russia, and Finland, this paper investigates the relationship between MNC subsidiary HRM practices, absorptive capacity and knowledge transfer. First, we examine the relationship between the application...... of specific HRM practices and the level of the absorptive capacity. Second, we suggest that absorptive capacity should be conceptualized as being comprised of both employees’ ability and motivation. Further, results indicate that both ability and motivation (absorptive capacity) are needed to facilitate...

  4. Methods of applied mathematics with a software overview

    CERN Document Server

    Davis, Jon H

    2016-01-01

    This textbook, now in its second edition, provides students with a firm grasp of the fundamental notions and techniques of applied mathematics as well as the software skills to implement them. The text emphasizes the computational aspects of problem solving as well as the limitations and implicit assumptions inherent in the formal methods. Readers are also given a sense of the wide variety of problems in which the presented techniques are useful. Broadly organized around the theme of applied Fourier analysis, the treatment covers classical applications in partial differential equations and boundary value problems, and a substantial number of topics associated with Laplace, Fourier, and discrete transform theories. Some advanced topics are explored in the final chapters such as short-time Fourier analysis and geometrically based transforms applicable to boundary value problems. The topics covered are useful in a variety of applied fields such as continuum mechanics, mathematical physics, control theory, and si...

  5. Region of interest methylation analysis: a comparison of MSP with MS-HRM and direct BSP.

    Science.gov (United States)

    Akika, Reem; Awada, Zainab; Mogharbil, Nahed; Zgheib, Nathalie K

    2017-07-01

    The aim of this study was to compare and contrast three DNA methylation methods of a specific region of interest (ROI): methylation-specific PCR (MSP), methylation-sensitive high resolution melting (MS-HRM) and direct bisulfite sequencing (BSP). The methylation of a CpG area in the promoter region of Estrogen receptor alpha (ESR1) was evaluated by these three methods with samples and standards of different methylation percentages. MSP data were neither reproducible nor sensitive, and the assay was not specific due to non-specific binding of primers. MS-HRM was highly reproducible and a step forward into categorizing the methylation status of the samples as percent ranges. Direct BSP was the most informative method regarding methylation percentage of each CpG site. Though not perfect, it was reproducible and sensitive. We recommend the use of either method depending on the research question and target amplicon, and provided that the designed primers and expected amplicons are within recommendations. If the research question targets a limited number of CpG sites and simple yes/no results are enough, MSP may be attempted. For short amplicons that are crowded with CpG sites and of single melting domain, MS-HRM may be the method of choice though it only indicates the overall methylation percentage of the entire amplicon. Although the assay is highly reproducible, being semi-quantitative makes it of lesser interest to study ROI methylation of samples with little methylation differences. Direct BSP is a step forward as it gives information about the methylation percentage at each CpG site.

  6. Which DTW Method Applied to Marine Univariate Time Series Imputation

    OpenAIRE

    Phan , Thi-Thu-Hong; Caillault , Émilie; Lefebvre , Alain; Bigand , André

    2017-01-01

    International audience; Missing data are ubiquitous in any domains of applied sciences. Processing datasets containing missing values can lead to a loss of efficiency and unreliable results, especially for large missing sub-sequence(s). Therefore, the aim of this paper is to build a framework for filling missing values in univariate time series and to perform a comparison of different similarity metrics used for the imputation task. This allows to suggest the most suitable methods for the imp...

  7. Applying Qualitative Research Methods to Narrative Knowledge Engineering

    OpenAIRE

    O'Neill, Brian; Riedl, Mark

    2014-01-01

    We propose a methodology for knowledge engineering for narrative intelligence systems, based on techniques used to elicit themes in qualitative methods research. Our methodology uses coding techniques to identify actions in natural language corpora, and uses these actions to create planning operators and procedural knowledge, such as scripts. In an iterative process, coders create a taxonomy of codes relevant to the corpus, and apply those codes to each element of that corpus. These codes can...

  8. APPLYING SPECTROSCOPIC METHODS ON ANALYSES OF HAZARDOUS WASTE

    OpenAIRE

    Dobrinić, Julijan; Kunić, Marija; Ciganj, Zlatko

    2000-01-01

    Abstract The paper presents results of measuring the content of heavy and other metals in waste samples from the hazardous waste disposal site of Sovjak near Rijeka. The preliminary design elaboration and the choice of the waste disposal sanification technology were preceded by the sampling and physico-chemical analyses of disposed waste, enabling its categorization. The following spectroscopic methods were applied on metal content analysis: Atomic absorption spectroscopy (AAS) and plas...

  9. A new method of AHP applied to personal credit evaluation

    Institute of Scientific and Technical Information of China (English)

    JIANG Ming-hui; XIONG Qi; CAO Jing

    2006-01-01

    This paper presents a new negative judgment matrix that combines the advantages of the reciprocal judgment matrix and the fuzzy complementary judgment matrix, and then puts forth the properties of this new matrix. In view of these properties, this paper derives a clear sequencing formula for the new negative judgment matrix, which improves the sequencing principle of AHP. Finally, this new method is applied to personal credit evaluation to show its advantages of conciseness and swiftness.

  10. Novel biodosimetry methods applied to victims of the Goiania accident

    International Nuclear Information System (INIS)

    Straume, T.; Langlois, R.G.; Lucas, J.; Jensen, R.H.; Bigbee, W.L.; Ramalho, A.T.; Brandao-Mello, C.E.

    1991-01-01

    Two biodosimetric methods under development at the Lawrence Livermore National Laboratory were applied to five persons accidentally exposed to a 137Cs source in Goiania, Brazil. The methods used were somatic null mutations at the glycophorin A locus detected as missing proteins on the surface of blood erythrocytes and chromosome translocations in blood lymphocytes detected using fluorescence in-situ hybridization. Biodosimetric results obtained approximately 1 y after the accident using these new and largely unvalidated methods are in general agreement with results obtained immediately after the accident using dicentric chromosome aberrations. Additional follow-up of Goiania accident victims will (1) help provide the information needed to validate these new methods for use in biodosimetry and (2) provide independent estimates of dose

  11. Newton-Krylov methods applied to nonequilibrium radiation diffusion

    International Nuclear Information System (INIS)

    Knoll, D.A.; Rider, W.J.; Olsen, G.L.

    1998-01-01

    The authors present results of applying a matrix-free Newton-Krylov method to a nonequilibrium radiation diffusion problem. Here, there is no use of operator splitting, and Newton's method is used to convert the nonlinearities within a time step. Since the nonlinear residual is formed, it is used to monitor convergence. It is demonstrated that a simple Picard-based linearization produces a sufficient preconditioning matrix for the Krylov method, thus elevating the need to form or store a Jacobian matrix for Newton's method. They discuss the possibility that the Newton-Krylov approach may allow larger time steps, without loss of accuracy, as compared to an operator split approach where nonlinearities are not converged within a time step

  12. High-Resolution Melting (HRM) of Hypervariable Mitochondrial DNA Regions for Forensic Science.

    Science.gov (United States)

    Dos Santos Rocha, Alípio; de Amorim, Isis Salviano Soares; Simão, Tatiana de Almeida; da Fonseca, Adenilson de Souza; Garrido, Rodrigo Grazinoli; Mencalha, Andre Luiz

    2018-03-01

    Forensic strategies commonly are proceeding by analysis of short tandem repeats (STRs); however, new additional strategies have been proposed for forensic science. Thus, this article standardized the high-resolution melting (HRM) of DNA for forensic analyzes. For HRM, mitochondrial DNA (mtDNA) from eight individuals were extracted from mucosa swabs by DNAzol reagent, samples were amplified by PCR and submitted to HRM analysis to identify differences in hypervariable (HV) regions I and II. To confirm HRM, all PCR products were DNA sequencing. The data suggest that is possible discriminate DNA from different samples by HRM curves. Also, uncommon dual-dissociation was identified in a single PCR product, increasing HRM analyzes by evaluation of melting peaks. Thus, HRM is accurate and useful to screening small differences in HVI and HVII regions from mtDNA and increase the efficiency of laboratory routines based on forensic genetics. © 2017 American Academy of Forensic Sciences.

  13. High-resolution melting-curve (HRM) analysis for C. meleagridis identification in stool samples.

    Science.gov (United States)

    Chelbi, Hanen; Essid, Rym; Jelassi, Refka; Bouzekri, Nesrine; Zidi, Ines; Ben Salah, Hamza; Mrad, Ilhem; Ben Sghaier, Ines; Abdelmalek, Rym; Aissa, Sameh; Bouratbine, Aida; Aoun, Karim

    2018-02-01

    Cryptosporidiosis represents a major public health problem. This infection, caused by a protozoan parasite of the genus Cryptosporidium, has been reported worldwide as a frequent cause of diarrhoea. In the immunocompetent host, the typical watery diarrhea can be self-limiting. However, it is severe and chronic, in the immunocompromised host and may cause death. Cryptosporidium spp. are coccidians, which complete their life cycle in both humans and animals. The two species C. hominis and C. parvum are the major cause of human infection. Compared to studies on C. hominis and C. parvum, only a few studies have developed methods to identify C. meleagridis. To develop a new real time PCR-coupled High resolution melting assay allowing the detection for C. meleagridis, in addition of the other dominant species (C. hominis and C. parvum). The polymorphic sequence on the dihydrofolate reductase gene (DHFR) of three species was sequenced to design primers pair and establish a sensitive real-time PCR coupled to a high-resolution melting-curve (HRM) analysis method, allowing the detection of Cryptosporidium sp. and discrimination between three prevalent species in Tunisia. We analyzed a collection of 42 archived human isolates of the three studied species. Real-time PCR coupled to HRM assay allowed detection of Cryptosporidium, using the new designed primers, and basing on melting profile, we can distinguish C. meleagridis species in addition to C. parvum and C. hominis. We developed a qPCR-HRM assay that allows Cryptosporidium genotyping. This method is sensitive and able to distinguish three Cryptosporidium species. Copyright © 2017. Published by Elsevier Ltd.

  14. Internationale virksomhedsopkøb - hvilken rolle spiller HRM?

    DEFF Research Database (Denmark)

    Neergaard, Helle

    1999-01-01

    The article focuses on the importance of cultural differences in international acquisitions. It illustrates how experience and knowledge about target companies determine how the acquiring firm handles HRM-issues and how a cultural due-diligence in the pre-acquisition phases will ease integration....

  15. Firm-level innovation activity, employee turnover and HRM practices

    DEFF Research Database (Denmark)

    Eriksson, Tor; Qin, Zhihua; Wang, Wenjing

    2014-01-01

    This paper examines the relationship between employee turnover, HRM practices and innovation in Chinese firms in five high technology sectors. We estimate hurdle negative binomial models for count data on survey data allowing for analyses of the extensive as well as intensive margins of firms' in...

  16. Competence and Employment Forum: Linking HRD and HRM

    Science.gov (United States)

    Siikaniemi, Lena

    2009-01-01

    Purpose: The purpose of this paper is to find a model for the individual employee, the employer, the supervisors and the personnel at HR to manage organizational and individual changes. The objective of the model is to manage the changes in parallel with the competence (HRD) and employment (HRM) aspects. Design/methodology/approach: The research…

  17. A Systems Model Comparing Australian and Chinese HRM Education

    Science.gov (United States)

    Davidson, Paul; Tsakissiris, Jane; Guo, Yuanyuan

    2017-01-01

    This paper explores the implications for learning design in HRM education in the 21st century. An open systems perspective is used to argue the importance of establishing productive relationships between academia, professional associations, regulators and industry (resource inputs) to support the creation of optimal learning environments (the…

  18. Professional HRM practices in family owned-managed enterprises

    NARCIS (Netherlands)

    J.M.P. de Kok (Jan); L.M. Uhlaner (Lorraine); A.R. Thurik (Roy)

    2003-01-01

    textabstractHuman resource management (HRM) has been defined as the “process of attracting, developing and maintaining a talented and energetic workforce to support organizational mission, objectives, and strategies” (Schermerhorn, 2001, p. 2400). Audretsch and Thurik (2000, 2001) argue that

  19. Strategic HRM for SMEs: Implications for Firms and Policy

    Science.gov (United States)

    Brand, Maryse J.; Bax, Erik H.

    2002-01-01

    This paper is on the growing importance of strategic human resource management (SHRM) for small and medium-sized enterprises (SMEs). Many small firms encounter serious human resource problems, while at the same time these human resources play a vital role in developing and sustaining their competitive advantages. In (S)HRM literature specific…

  20. Examining the relationship between HRM and innovative work behaviour

    NARCIS (Netherlands)

    Veenendaal, Andre; Bos-Nehles, Anna Christina; van Velzen, Martijn; Looise, Jan C.

    2011-01-01

    The purpose of this paper is to study how HRM is related to individual innovation. As an operationalization of individual innovation, we will study the construct of innovative work behaviour (IWB). IWB is about the behaviour of an individual to intentionally introduce new ideas, processes or

  1. GPS surveying method applied to terminal area navigation flight experiments

    Energy Technology Data Exchange (ETDEWEB)

    Murata, M; Shingu, H; Satsushima, K; Tsuji, T; Ishikawa, K; Miyazawa, Y; Uchida, T [National Aerospace Laboratory, Tokyo (Japan)

    1993-03-01

    With an objective of evaluating accuracy of new landing and navigation systems such as microwave landing guidance system and global positioning satellite (GPS) system, flight experiments are being carried out using experimental aircraft. This aircraft mounts a GPS and evaluates its accuracy by comparing the standard orbits spotted by a Kalman filter from the laser tracing data on the aircraft with the navigation results. The GPS outputs position and speed information from an earth-centered-earth-fixed system called the World Geodetic System, 1984 (WGS84). However, in order to compare the navigation results with output from a reference orbit sensor or other navigation sensor, it is necessary to structure a high-precision reference coordinates system based on the WGS84. A method that applies the GPS phase interference measurement for this problem was proposed, and used actually in analyzing a flight experiment data. As referred to a case of the method having been applied to evaluating an independent navigation accuracy, the method was verified sufficiently effective and reliable not only in navigation method analysis, but also in the aspect of navigational operations. 12 refs., 10 figs., 5 tabs.

  2. Methods for model selection in applied science and engineering.

    Energy Technology Data Exchange (ETDEWEB)

    Field, Richard V., Jr.

    2004-10-01

    Mathematical models are developed and used to study the properties of complex systems and/or modify these systems to satisfy some performance requirements in just about every area of applied science and engineering. A particular reason for developing a model, e.g., performance assessment or design, is referred to as the model use. Our objective is the development of a methodology for selecting a model that is sufficiently accurate for an intended use. Information on the system being modeled is, in general, incomplete, so that there may be two or more models consistent with the available information. The collection of these models is called the class of candidate models. Methods are developed for selecting the optimal member from a class of candidate models for the system. The optimal model depends on the available information, the selected class of candidate models, and the model use. Classical methods for model selection, including the method of maximum likelihood and Bayesian methods, as well as a method employing a decision-theoretic approach, are formulated to select the optimal model for numerous applications. There is no requirement that the candidate models be random. Classical methods for model selection ignore model use and require data to be available. Examples are used to show that these methods can be unreliable when data is limited. The decision-theoretic approach to model selection does not have these limitations, and model use is included through an appropriate utility function. This is especially important when modeling high risk systems, where the consequences of using an inappropriate model for the system can be disastrous. The decision-theoretic method for model selection is developed and applied for a series of complex and diverse applications. These include the selection of the: (1) optimal order of the polynomial chaos approximation for non-Gaussian random variables and stationary stochastic processes, (2) optimal pressure load model to be

  3. Analysis of concrete beams using applied element method

    Science.gov (United States)

    Lincy Christy, D.; Madhavan Pillai, T. M.; Nagarajan, Praveen

    2018-03-01

    The Applied Element Method (AEM) is a displacement based method of structural analysis. Some of its features are similar to that of Finite Element Method (FEM). In AEM, the structure is analysed by dividing it into several elements similar to FEM. But, in AEM, elements are connected by springs instead of nodes as in the case of FEM. In this paper, background to AEM is discussed and necessary equations are derived. For illustrating the application of AEM, it has been used to analyse plain concrete beam of fixed support condition. The analysis is limited to the analysis of 2-dimensional structures. It was found that the number of springs has no much influence on the results. AEM could predict deflection and reactions with reasonable degree of accuracy.

  4. The Lattice Boltzmann Method applied to neutron transport

    International Nuclear Information System (INIS)

    Erasmus, B.; Van Heerden, F. A.

    2013-01-01

    In this paper the applicability of the Lattice Boltzmann Method to neutron transport is investigated. One of the main features of the Lattice Boltzmann method is the simultaneous discretization of the phase space of the problem, whereby particles are restricted to move on a lattice. An iterative solution of the operator form of the neutron transport equation is presented here, with the first collision source as the starting point of the iteration scheme. A full description of the discretization scheme is given, along with the quadrature set used for the angular discretization. An angular refinement scheme is introduced to increase the angular coverage of the problem phase space and to mitigate lattice ray effects. The method is applied to a model problem to investigate its applicability to neutron transport and the results are compared to a reference solution calculated, using MCNP. (authors)

  5. Advanced methods for image registration applied to JET videos

    Energy Technology Data Exchange (ETDEWEB)

    Craciunescu, Teddy, E-mail: teddy.craciunescu@jet.uk [EURATOM-MEdC Association, NILPRP, Bucharest (Romania); Murari, Andrea [Consorzio RFX, Associazione EURATOM-ENEA per la Fusione, Padova (Italy); Gelfusa, Michela [Associazione EURATOM-ENEA – University of Rome “Tor Vergata”, Roma (Italy); Tiseanu, Ion; Zoita, Vasile [EURATOM-MEdC Association, NILPRP, Bucharest (Romania); Arnoux, Gilles [EURATOM/CCFE Fusion Association, Culham Science Centre, Abingdon, Oxon (United Kingdom)

    2015-10-15

    Graphical abstract: - Highlights: • Development of an image registration method for JET IR and fast visible cameras. • Method based on SIFT descriptors and coherent point drift points set registration technique. • Method able to deal with extremely noisy images and very low luminosity images. • Computation time compatible with the inter-shot analysis. - Abstract: The last years have witnessed a significant increase in the use of digital cameras on JET. They are routinely applied for imaging in the IR and visible spectral regions. One of the main technical difficulties in interpreting the data of camera based diagnostics is the presence of movements of the field of view. Small movements occur due to machine shaking during normal pulses while large ones may arise during disruptions. Some cameras show a correlation of image movement with change of magnetic field strength. For deriving unaltered information from the videos and for allowing correct interpretation an image registration method, based on highly distinctive scale invariant feature transform (SIFT) descriptors and on the coherent point drift (CPD) points set registration technique, has been developed. The algorithm incorporates a complex procedure for rejecting outliers. The method has been applied for vibrations correction to videos collected by the JET wide angle infrared camera and for the correction of spurious rotations in the case of the JET fast visible camera (which is equipped with an image intensifier). The method has proved to be able to deal with the images provided by this camera frequently characterized by low contrast and a high level of blurring and noise.

  6. Learning to work with interdependencies effectively: the case of the HRM forum of the suppliers teams at Volvo Cars Gent

    OpenAIRE

    LAMBRECHTS, Frank; Taillieu, Tharsi; Sips, Koen

    2010-01-01

    Purpose The purpose of this paper is to profile the way in which Volvo Cars Gent (VCG) Belgium and its suppliers succeed in managing their interdependencies on HRM issues through a shared HRM collaborative, called the Suppliers Team Volvo Cars HRM forum (STVC-HRM). Design/methodology/approach - A case study approach is used to develop understanding of the critical factors that contribute to the forum's success. Findings - It was found that the critical success factors concern the way STVC-HRM...

  7. Classification of Specialized Farms Applying Multivariate Statistical Methods

    Directory of Open Access Journals (Sweden)

    Zuzana Hloušková

    2017-01-01

    Full Text Available Classification of specialized farms applying multivariate statistical methods The paper is aimed at application of advanced multivariate statistical methods when classifying cattle breeding farming enterprises by their economic size. Advantage of the model is its ability to use a few selected indicators compared to the complex methodology of current classification model that requires knowledge of detailed structure of the herd turnover and structure of cultivated crops. Output of the paper is intended to be applied within farm structure research focused on future development of Czech agriculture. As data source, the farming enterprises database for 2014 has been used, from the FADN CZ system. The predictive model proposed exploits knowledge of actual size classes of the farms tested. Outcomes of the linear discriminatory analysis multifactor classification method have supported the chance of filing farming enterprises in the group of Small farms (98 % filed correctly, and the Large and Very Large enterprises (100 % filed correctly. The Medium Size farms have been correctly filed at 58.11 % only. Partial shortages of the process presented have been found when discriminating Medium and Small farms.

  8. Metrological evaluation of characterization methods applied to nuclear fuels

    International Nuclear Information System (INIS)

    Faeda, Kelly Cristina Martins; Lameiras, Fernando Soares; Camarano, Denise das Merces; Ferreira, Ricardo Alberto Neto; Migliorini, Fabricio Lima; Carneiro, Luciana Capanema Silva; Silva, Egonn Hendrigo Carvalho

    2010-01-01

    In manufacturing the nuclear fuel, characterizations are performed in order to assure the minimization of harmful effects. The uranium dioxide is the most used substance as nuclear reactor fuel because of many advantages, such as: high stability even when it is in contact with water at high temperatures, high fusion point, and high capacity to retain fission products. Several methods are used for characterization of nuclear fuels, such as thermogravimetric analysis for the ratio O / U, penetration-immersion method, helium pycnometer and mercury porosimetry for the density and porosity, BET method for the specific surface, chemical analyses for relevant impurities, and the laser flash method for thermophysical properties. Specific tools are needed to control the diameter and the sphericity of the microspheres and the properties of the coating layers (thickness, density, and degree of anisotropy). Other methods can also give information, such as scanning and transmission electron microscopy, X-ray diffraction, microanalysis, and mass spectroscopy of secondary ions for chemical analysis. The accuracy of measurement and level of uncertainty of the resulting data are important. This work describes a general metrological characterization of some techniques applied to the characterization of nuclear fuel. Sources of measurement uncertainty were analyzed. The purpose is to summarize selected properties of UO 2 that have been studied by CDTN in a program of fuel development for Pressurized Water Reactors (PWR). The selected properties are crucial for thermalhydraulic codes to study basic design accidents. The thermal characterization (thermal diffusivity and thermal conductivity) and the penetration immersion method (density and open porosity) of UO 2 samples were focused. The thermal characterization of UO 2 samples was determined by the laser flash method between room temperature and 448 K. The adaptive Monte Carlo Method was used to obtain the endpoints of the

  9. Nuclear and nuclear related analytical methods applied in environmental research

    International Nuclear Information System (INIS)

    Popescu, Ion V.; Gheboianu, Anca; Bancuta, Iulian; Cimpoca, G. V; Stihi, Claudia; Radulescu, Cristiana; Oros Calin; Frontasyeva, Marina; Petre, Marian; Dulama, Ioana; Vlaicu, G.

    2010-01-01

    Nuclear Analytical Methods can be used for research activities on environmental studies like water quality assessment, pesticide residues, global climatic change (transboundary), pollution and remediation. Heavy metal pollution is a problem associated with areas of intensive industrial activity. In this work the moss bio monitoring technique was employed to study the atmospheric deposition in Dambovita County Romania. Also, there were used complementary nuclear and atomic analytical methods: Neutron Activation Analysis (NAA), Atomic Absorption Spectrometry (AAS) and Inductively Coupled Plasma Atomic Emission Spectrometry (ICP-AES). These high sensitivity analysis methods were used to determine the chemical composition of some samples of mosses placed in different areas with different pollution industrial sources. The concentrations of Cr, Fe, Mn, Ni and Zn were determined. The concentration of Fe from the same samples was determined using all these methods and we obtained a very good agreement, in statistical limits, which demonstrate the capability of these analytical methods to be applied on a large spectrum of environmental samples with the same results. (authors)

  10. Applied systems ecology: models, data, and statistical methods

    Energy Technology Data Exchange (ETDEWEB)

    Eberhardt, L L

    1976-01-01

    In this report, systems ecology is largely equated to mathematical or computer simulation modelling. The need for models in ecology stems from the necessity to have an integrative device for the diversity of ecological data, much of which is observational, rather than experimental, as well as from the present lack of a theoretical structure for ecology. Different objectives in applied studies require specialized methods. The best predictive devices may be regression equations, often non-linear in form, extracted from much more detailed models. A variety of statistical aspects of modelling, including sampling, are discussed. Several aspects of population dynamics and food-chain kinetics are described, and it is suggested that the two presently separated approaches should be combined into a single theoretical framework. It is concluded that future efforts in systems ecology should emphasize actual data and statistical methods, as well as modelling.

  11. Analysis of Brick Masonry Wall using Applied Element Method

    Science.gov (United States)

    Lincy Christy, D.; Madhavan Pillai, T. M.; Nagarajan, Praveen

    2018-03-01

    The Applied Element Method (AEM) is a versatile tool for structural analysis. Analysis is done by discretising the structure as in the case of Finite Element Method (FEM). In AEM, elements are connected by a set of normal and shear springs instead of nodes. AEM is extensively used for the analysis of brittle materials. Brick masonry wall can be effectively analyzed in the frame of AEM. The composite nature of masonry wall can be easily modelled using springs. The brick springs and mortar springs are assumed to be connected in series. The brick masonry wall is analyzed and failure load is determined for different loading cases. The results were used to find the best aspect ratio of brick to strengthen brick masonry wall.

  12. Thermally stimulated current method applied to highly irradiated silicon diodes

    CERN Document Server

    Pintilie, I; Pintilie, I; Moll, Michael; Fretwurst, E; Lindström, G

    2002-01-01

    We propose an improved method for the analysis of Thermally Stimulated Currents (TSC) measured on highly irradiated silicon diodes. The proposed TSC formula for the evaluation of a set of TSC spectra obtained with different reverse biases leads not only to the concentration of electron and hole traps visible in the spectra but also gives an estimation for the concentration of defects which not give rise to a peak in the 30-220 K TSC temperature range (very shallow or very deep levels). The method is applied to a diode irradiated with a neutron fluence of phi sub n =1.82x10 sup 1 sup 3 n/cm sup 2.

  13. Hybrid electrokinetic method applied to mix contaminated soil

    Energy Technology Data Exchange (ETDEWEB)

    Mansour, H.; Maria, E. [Dept. of Building Civil and Environmental Engineering, Concordia Univ., Montreal (Canada)

    2001-07-01

    Several industrials and municipal areas in North America are contaminated with heavy metals and petroleum products. This mix contamination presents a particularly difficult task for remediation when is exposed in clayey soil. The objective of this research was to find a method to cleanup mix contaminated clayey soils. Finally, a multifunctional hybrid electrokinetic method was investigated. Clayey soil was contaminated with lead and nickel (heavy metals) at the level of 1000 ppm and phenanthrene (PAH) of 600 ppm. Electrokinetic surfactant supply system was applied to mobilize, transport and removal of phenanthrene. A chelation agent (EDTA) was also electrokinetically supplied to mobilize heavy metals. The studies were performed on 8 lab scale electrokinetic cells. The mix contaminated clayey soil was subjected to DC total voltage gradient of 0.3 V/cm. Supplied liquids (surfactant and EDTA) were introduced in different periods of time (22 days, 42 days) in order to optimize the most excessive removal of contaminants. The ph, electrical parameters, volume supplied, and volume discharged was monitored continuously during each experiment. At the end of these tests soil and cathalyte were subjected to physico-chemical analysis. The paper discusses results of experiments including the optimal energy use, removal efficiency of phenanthrene, as well, transport and removal of heavy metals. The results of this study can be applied for in-situ hybrid electrokinetic technology to remediate clayey sites contaminated with petroleum product mixed with heavy metals (e.g. manufacture Gas Plant Sites). (orig.)

  14. A Multifactorial Analysis of Reconstruction Methods Applied After Total Gastrectomy

    Directory of Open Access Journals (Sweden)

    Oktay Büyükaşık

    2010-12-01

    Full Text Available Aim: The aim of this study was to evaluate the reconstruction methods applied after total gastrectomy in terms of postoperative symptomology and nutrition. Methods: This retrospective study was conducted on 31 patients who underwent total gastrectomy due to gastric cancer in 2. Clinic of General Surgery, SSK Ankara Training Hospital. 6 different reconstruction methods were used and analyzed in terms of age, sex and postoperative complications. One from esophagus and two biopsy specimens from jejunum were taken through upper gastrointestinal endoscopy from all cases, and late period morphological and microbiological changes were examined. Postoperative weight change, dumping symptoms, reflux esophagitis, solid/liquid dysphagia, early satiety, postprandial pain, diarrhea and anorexia were assessed. Results: Of 31 patients,18 were males and 13 females; the youngest one was 33 years old, while the oldest- 69 years old. It was found that reconstruction without pouch was performed in 22 cases and with pouch in 9 cases. Early satiety, postprandial pain, dumping symptoms, diarrhea and anemia were found most commonly in cases with reconstruction without pouch. The rate of bacterial colonization of the jejunal mucosa was identical in both groups. Reflux esophagitis was most commonly seen in omega esophagojejunostomy (EJ, while the least-in Roux-en-Y, Tooley and Tanner 19 EJ. Conclusion: Reconstruction with pouch performed after total gastrectomy is still a preferable method. (The Medical Bulletin of Haseki 2010; 48:126-31

  15. The high resolution melting analysis (HRM) as a molecular tool for monitoring parasites of the wildlife.

    Science.gov (United States)

    Héritier, Laurent; Verneau, Olivier; Breuil, Gregory; Meistertzheim, Anne-Leila

    2017-04-01

    In an interconnected world, the international pet trade on wild animals is becoming increasingly important. As a consequence, non-native parasite species are introduced, which affect the health of wildlife and contribute to the loss of biodiversity. Because the investigation of parasite diversity within vulnerable host species implies the molecular identification of large samples of parasite eggs, the sequencing of DNA barcodes is time-consuming and costly. Thereby, the objectives of our study were to apply the high resolution melting (HRM) approach for species determination from pools of parasite eggs. Molecular assays were validated on flatworm parasites (polystomes) infecting the Mediterranean pond turtle Mauremys leprosa and the invasive red-eared slider Trachemys scripta elegans in French natural environments. HRM analysis results indicated that double or multiple parasitic infections could be detected from wild animal populations. They also showed that the cycle of parasite eggs production was not regular over time and may depend on several factors, among which the ecological niche and the target species. Thereby, monitoring parasites from wild endangered animals implies periodic parasitological surveys to avoid false negative diagnostics, based solely on eggs production.

  16. High-resolution melt-curve analysis of random amplified polymorphic DNA (RAPD-HRM) for the characterisation of pathogenic leptospires: intra-serovar divergence, inter-serovar convergence, and evidence of attenuation in Leptospira reference collections.

    Science.gov (United States)

    Tulsiani, S M; Craig, S B; Graham, G C; Cobbold, R C; Dohnt, M F; Burns, M-A; Jansen, C C; Leung, L K-P; Field, H E; Smythe, L D

    2010-07-01

    High-resolution melt-curve analysis of random amplified polymorphic DNA (RAPD-HRM) is a novel technology that has emerged as a possible method to characterise leptospires to serovar level. RAPD-HRM has recently been used to measure intra-serovar convergence between strains of the same serovar as well as inter-serovar divergence between strains of different serovars. The results indicate that intra-serovar heterogeneity and inter-serovar homogeneity may limit the application of RAPD-HRM in routine diagnostics. They also indicate that genetic attenuation of aged, high-passage-number isolates could undermine the use of RAPD-HRM or any other molecular technology. Such genetic attenuation may account for a general decrease seen in titres of rabbit hyperimmune antibodies over time. Before RAPD-HRM can be further advanced as a routine diagnostic tool, strains more representative of the wild-type serovars of a given region need to be identified. Further, RAPD-HRM analysis of reference strains indicates that the routine renewal of reference collections, with new isolates, may be needed to maintain the genetic integrity of the collections.

  17. Single-Case Designs and Qualitative Methods: Applying a Mixed Methods Research Perspective

    Science.gov (United States)

    Hitchcock, John H.; Nastasi, Bonnie K.; Summerville, Meredith

    2010-01-01

    The purpose of this conceptual paper is to describe a design that mixes single-case (sometimes referred to as single-subject) and qualitative methods, hereafter referred to as a single-case mixed methods design (SCD-MM). Minimal attention has been given to the topic of applying qualitative methods to SCD work in the literature. These two…

  18. Analytical methods applied to diverse types of Brazilian propolis

    Directory of Open Access Journals (Sweden)

    Marcucci Maria

    2011-06-01

    Full Text Available Abstract Propolis is a bee product, composed mainly of plant resins and beeswax, therefore its chemical composition varies due to the geographic and plant origins of these resins, as well as the species of bee. Brazil is an important supplier of propolis on the world market and, although green colored propolis from the southeast is the most known and studied, several other types of propolis from Apis mellifera and native stingless bees (also called cerumen can be found. Propolis is usually consumed as an extract, so the type of solvent and extractive procedures employed further affect its composition. Methods used for the extraction; analysis the percentage of resins, wax and insoluble material in crude propolis; determination of phenolic, flavonoid, amino acid and heavy metal contents are reviewed herein. Different chromatographic methods applied to the separation, identification and quantification of Brazilian propolis components and their relative strengths are discussed; as well as direct insertion mass spectrometry fingerprinting. Propolis has been used as a popular remedy for several centuries for a wide array of ailments. Its antimicrobial properties, present in propolis from different origins, have been extensively studied. But, more recently, anti-parasitic, anti-viral/immune stimulating, healing, anti-tumor, anti-inflammatory, antioxidant and analgesic activities of diverse types of Brazilian propolis have been evaluated. The most common methods employed and overviews of their relative results are presented.

  19. Teaching organization theory for healthcare management: three applied learning methods.

    Science.gov (United States)

    Olden, Peter C

    2006-01-01

    Organization theory (OT) provides a way of seeing, describing, analyzing, understanding, and improving organizations based on patterns of organizational design and behavior (Daft 2004). It gives managers models, principles, and methods with which to diagnose and fix organization structure, design, and process problems. Health care organizations (HCOs) face serious problems such as fatal medical errors, harmful treatment delays, misuse of scarce nurses, costly inefficiency, and service failures. Some of health care managers' most critical work involves designing and structuring their organizations so their missions, visions, and goals can be achieved-and in some cases so their organizations can survive. Thus, it is imperative that graduate healthcare management programs develop effective approaches for teaching OT to students who will manage HCOs. Guided by principles of education, three applied teaching/learning activities/assignments were created to teach OT in a graduate healthcare management program. These educationalmethods develop students' competency with OT applied to HCOs. The teaching techniques in this article may be useful to faculty teaching graduate courses in organization theory and related subjects such as leadership, quality, and operation management.

  20. Six Sigma methods applied to cryogenic coolers assembly line

    Science.gov (United States)

    Ventre, Jean-Marc; Germain-Lacour, Michel; Martin, Jean-Yves; Cauquil, Jean-Marc; Benschop, Tonny; Griot, René

    2009-05-01

    Six Sigma method have been applied to manufacturing process of a rotary Stirling cooler: RM2. Name of the project is NoVa as main goal of the Six Sigma approach is to reduce variability (No Variability). Project has been based on the DMAIC guideline following five stages: Define, Measure, Analyse, Improve, Control. Objective has been set on the rate of coolers succeeding performance at first attempt with a goal value of 95%. A team has been gathered involving people and skills acting on the RM2 manufacturing line. Measurement System Analysis (MSA) has been applied to test bench and results after R&R gage show that measurement is one of the root cause for variability in RM2 process. Two more root causes have been identified by the team after process mapping analysis: regenerator filling factor and cleaning procedure. Causes for measurement variability have been identified and eradicated as shown by new results from R&R gage. Experimental results show that regenerator filling factor impacts process variability and affects yield. Improved process haven been set after new calibration process for test bench, new filling procedure for regenerator and an additional cleaning stage have been implemented. The objective for 95% coolers succeeding performance test at first attempt has been reached and kept for a significant period. RM2 manufacturing process is now managed according to Statistical Process Control based on control charts. Improvement in process capability have enabled introduction of sample testing procedure before delivery.

  1. Metrological evaluation of characterization methods applied to nuclear fuels

    Energy Technology Data Exchange (ETDEWEB)

    Faeda, Kelly Cristina Martins; Lameiras, Fernando Soares; Camarano, Denise das Merces; Ferreira, Ricardo Alberto Neto; Migliorini, Fabricio Lima; Carneiro, Luciana Capanema Silva; Silva, Egonn Hendrigo Carvalho, E-mail: kellyfisica@gmail.co, E-mail: fernando.lameiras@pq.cnpq.b, E-mail: dmc@cdtn.b, E-mail: ranf@cdtn.b, E-mail: flmigliorini@hotmail.co, E-mail: lucsc@hotmail.co, E-mail: egonn@ufmg.b [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil)

    2010-07-01

    In manufacturing the nuclear fuel, characterizations are performed in order to assure the minimization of harmful effects. The uranium dioxide is the most used substance as nuclear reactor fuel because of many advantages, such as: high stability even when it is in contact with water at high temperatures, high fusion point, and high capacity to retain fission products. Several methods are used for characterization of nuclear fuels, such as thermogravimetric analysis for the ratio O / U, penetration-immersion method, helium pycnometer and mercury porosimetry for the density and porosity, BET method for the specific surface, chemical analyses for relevant impurities, and the laser flash method for thermophysical properties. Specific tools are needed to control the diameter and the sphericity of the microspheres and the properties of the coating layers (thickness, density, and degree of anisotropy). Other methods can also give information, such as scanning and transmission electron microscopy, X-ray diffraction, microanalysis, and mass spectroscopy of secondary ions for chemical analysis. The accuracy of measurement and level of uncertainty of the resulting data are important. This work describes a general metrological characterization of some techniques applied to the characterization of nuclear fuel. Sources of measurement uncertainty were analyzed. The purpose is to summarize selected properties of UO{sub 2} that have been studied by CDTN in a program of fuel development for Pressurized Water Reactors (PWR). The selected properties are crucial for thermalhydraulic codes to study basic design accidents. The thermal characterization (thermal diffusivity and thermal conductivity) and the penetration immersion method (density and open porosity) of UO{sub 2} samples were focused. The thermal characterization of UO{sub 2} samples was determined by the laser flash method between room temperature and 448 K. The adaptive Monte Carlo Method was used to obtain the endpoints of

  2. Applying systems ergonomics methods in sport: A systematic review.

    Science.gov (United States)

    Hulme, Adam; Thompson, Jason; Plant, Katherine L; Read, Gemma J M; Mclean, Scott; Clacy, Amanda; Salmon, Paul M

    2018-04-16

    As sports systems become increasingly more complex, competitive, and technology-centric, there is a greater need for systems ergonomics methods to consider the performance, health, and safety of athletes in context with the wider settings in which they operate. Therefore, the purpose of this systematic review was to identify and critically evaluate studies which have applied a systems ergonomics research approach in the context of sports performance and injury management. Five databases (PubMed, Scopus, ScienceDirect, Web of Science, and SPORTDiscus) were searched for the dates 01 January 1990 to 01 August 2017, inclusive, for original peer-reviewed journal articles and conference papers. Reported analyses were underpinned by a recognised systems ergonomics method, and study aims were related to the optimisation of sports performance (e.g. communication, playing style, technique, tactics, or equipment), and/or the management of sports injury (i.e. identification, prevention, or treatment). A total of seven articles were identified. Two articles were focussed on understanding and optimising sports performance, whereas five examined sports injury management. The methods used were the Event Analysis of Systemic Teamwork, Cognitive Work Analysis (the Work Domain Analysis Abstraction Hierarchy), Rasmussen's Risk Management Framework, and the Systems Theoretic Accident Model and Processes method. The individual sport application was distance running, whereas the team sports contexts examined were cycling, football, Australian Football League, and rugby union. The included systems ergonomics applications were highly flexible, covering both amateur and elite sports contexts. The studies were rated as valuable, providing descriptions of injury controls and causation, the factors influencing injury management, the allocation of responsibilities for injury prevention, as well as the factors and their interactions underpinning sports performance. Implications and future

  3. The virtual fields method applied to spalling tests on concrete

    Directory of Open Access Journals (Sweden)

    Forquin P.

    2012-08-01

    Full Text Available For one decade spalling techniques based on the use of a metallic Hopkinson bar put in contact with a concrete sample have been widely employed to characterize the dynamic tensile strength of concrete at strain-rates ranging from a few tens to two hundreds of s−1. However, the processing method mainly based on the use of the velocity profile measured on the rear free surface of the sample (Novikov formula remains quite basic and an identification of the whole softening behaviour of the concrete is out of reach. In the present paper a new processing method is proposed based on the use of the Virtual Fields Method (VFM. First, a digital high speed camera is used to record the pictures of a grid glued on the specimen. Next, full-field measurements are used to obtain the axial displacement field at the surface of the specimen. Finally, a specific virtual field has been defined in the VFM equation to use the acceleration map as an alternative ‘load cell’. This method applied to three spalling tests allowed to identify Young’s modulus during the test. It was shown that this modulus is constant during the initial compressive part of the test and decreases in the tensile part when micro-damage exists. It was also shown that in such a simple inertial test, it was possible to reconstruct average axial stress profiles using only the acceleration data. Then, it was possible to construct local stress-strain curves and derive a tensile strength value.

  4. Linking HRM and Knowledge Transfer via Individual-level Mechanisms

    DEFF Research Database (Denmark)

    Minbaeva, Dana; Mäkelä, Kristiina; Rabbiosi, Larissa

    2012-01-01

    In response to recent calls for more research on micro-foundations, we seek to link human resource management (HRM) and knowledge transfer through individual-level mechanisms, arguing that individual-level conditions of action influence the extent to which employees engage in knowledge exchange. We...... examine four such conditions empirically using data from 811 employees in three Danish multinational corporations (MNCs). Our findings suggest that individual-level perceptions of organizational commitment to knowledge sharing, and extrinsic motivation, directly influence the extent to which employees...... engage in firm-internal knowledge exchange. We also find that intrinsic motivation and engagement in social interaction significantly mediate the relationship between perceived organizational commitment and knowledge exchange. Given that HRM can influence such conditions through an overall signaling...

  5. Optimizing the high-resolution manometry (HRM) study protocol.

    Science.gov (United States)

    Patel, A; Ding, A; Mirza, F; Gyawali, C P

    2015-02-01

    Intolerance of the esophageal manometry catheter may prolong high-resolution manometry (HRM) studies and increase patient distress. We assessed the impact of obtaining the landmark phase at the end of the study when the patient has acclimatized to the HRM catheter. 366 patients (mean age 55.4 ± 0.8 years, 62.0% female) undergoing esophageal HRM over a 1-year period were studied. The standard protocol consisted of the landmark phase, 10 5 mL water swallows 20-30 s apart, and multiple rapid swallows where 4-6 2 mL swallows were administered in rapid succession. The modified protocol consisted of the landmark phase at the end of the study after test swallows. Study duration, technical characteristics, indications, and motor findings were compared between standard and modified protocols. Of the 366 patients, 89.6% underwent the standard protocol (study duration 12.9 ± 0.3 min). In 10.4% with poor catheter tolerance undergoing the modified protocol, study duration was significantly longer (15.6 ± 1.0 min, p = 0.004) despite similar duration of study maneuvers. Only elevated upper esophageal sphincter basal pressures at the beginning of the study segregated modified protocol patients. The 95th percentile time to landmark phase in the standard protocol patients was 6.1 min; as many as 31.4% of modified protocol patients could not obtain their first study maneuver within this period (p = 0.0003). Interpretation was not impacted by shifting the landmark phase to the end of the study. Modification of the HRM study protocol with the landmark phase obtained at the end of the study optimizes study duration without compromising quality. © 2014 John Wiley & Sons Ltd.

  6. Flood Hazard Mapping by Applying Fuzzy TOPSIS Method

    Science.gov (United States)

    Han, K. Y.; Lee, J. Y.; Keum, H.; Kim, B. J.; Kim, T. H.

    2017-12-01

    There are lots of technical methods to integrate various factors for flood hazard mapping. The purpose of this study is to suggest the methodology of integrated flood hazard mapping using MCDM(Multi Criteria Decision Making). MCDM problems involve a set of alternatives that are evaluated on the basis of conflicting and incommensurate criteria. In this study, to apply MCDM to assessing flood risk, maximum flood depth, maximum velocity, and maximum travel time are considered as criterion, and each applied elements are considered as alternatives. The scheme to find the efficient alternative closest to a ideal value is appropriate way to assess flood risk of a lot of element units(alternatives) based on various flood indices. Therefore, TOPSIS which is most commonly used MCDM scheme is adopted to create flood hazard map. The indices for flood hazard mapping(maximum flood depth, maximum velocity, and maximum travel time) have uncertainty concerning simulation results due to various values according to flood scenario and topographical condition. These kind of ambiguity of indices can cause uncertainty of flood hazard map. To consider ambiguity and uncertainty of criterion, fuzzy logic is introduced which is able to handle ambiguous expression. In this paper, we made Flood Hazard Map according to levee breach overflow using the Fuzzy TOPSIS Technique. We confirmed the areas where the highest grade of hazard was recorded through the drawn-up integrated flood hazard map, and then produced flood hazard map can be compared them with those indicated in the existing flood risk maps. Also, we expect that if we can apply the flood hazard map methodology suggested in this paper even to manufacturing the current flood risk maps, we will be able to make a new flood hazard map to even consider the priorities for hazard areas, including more varied and important information than ever before. Keywords : Flood hazard map; levee break analysis; 2D analysis; MCDM; Fuzzy TOPSIS

  7. Strategic HRM in Building Micro-Foundations of Organizational Knowledge-Based Performance

    DEFF Research Database (Denmark)

    Minbaeva, Dana

    2013-01-01

    Strategic HRM research has a strong potential to further our understanding of how organizational knowledge processes influence performance at various analytical levels. Drawing on ability–motivation–opportunity research and linking it to knowledge sharing behaviors, we discuss the micro......-foundations in the link between strategic HRM practices and knowledge-based organizational performance. We thus describe a research agenda for future micro-foundational research that links strategic HRM and knowledge-based performance....

  8. Employee Perceptions of HRM and TQM and the Effects on Satisfaction and INtention to Leave

    OpenAIRE

    Boselie, J.P.P.E.F.; van der Wiele, A.

    2001-01-01

    textabstractThere is a growing interest in theory and in practice with regard to the relationship between human resource management [HRM] and total quality management [TQM] as well as the relationship between these two perspectives and business performances. Empirical research suggests significant effects of HRM/TQM on the performances of an organisation. The majority of research in this area is focused on the effects of HRM/TQM at the organisational level. Research on the perceptions of indi...

  9. IMPACT OF HRM PRACTICES ON EMPLOYEE SATISFACTION IN BANKING SECTOR IN CHITTOOR DISTRICT

    OpenAIRE

    P.Suresh; Dr.T.Narayana Reddy

    2017-01-01

    Employee satisfaction plays a key role on both the individual and organisation. Employee satisfaction will result in improving organisation productivity. The present study is carried out to comprehend the effect of HRM Practices in banking sector. This study is based on both primary and secondary data, reviewing the literatures related to HRM Practices, employee satisfaction, in banks of chittoor district. The objective of the study focuses on understanding Effect of HRM practices on Employee...

  10. Esophagogastric Junction pressure morphology: comparison between a station pull-through and real-time 3D-HRM representation.

    Science.gov (United States)

    Nicodème, F; Lin, Z; Pandolfino, J E; Kahrilas, P J

    2013-09-01

    Esophagogastric junction (EGJ) competence is the fundamental defense against reflux making it of great clinical significance. However, characterizing EGJ competence with conventional manometric methodologies has been confounded by its anatomic and physiological complexity. Recent technological advances in miniaturization and electronics have led to the development of a novel device that may overcome these challenges. Nine volunteer subjects were studied with a novel 3D-HRM device providing 7.5 mm axial and 45° radial pressure resolution within the EGJ. Real-time measurements were made at rest and compared to simulations of a conventional pull-through made with the same device. Moreover, 3D-HRM recordings were analyzed to differentiate contributing pressure signals within the EGJ attributable to lower esophageal sphincter (LES), diaphragm, and vasculature. 3D-HRM recordings suggested that sphincter length assessed by a pull-through method greatly exaggerated the estimate of LES length by failing to discriminate among circumferential contractile pressure and asymmetric extrinsic pressure signals attributable to diaphragmatic and vascular structures. Real-time 3D EGJ recordings found that the dominant constituents of EGJ pressure at rest were attributable to the diaphragm. 3D-HRM permits real-time recording of EGJ pressure morphology facilitating analysis of the EGJ constituents responsible for its function as a reflux barrier making it a promising tool in the study of GERD pathophysiology. The enhanced axial and radial recording resolution of the device should facilitate further studies to explore perturbations in the physiological constituents of EGJ pressure in health and disease. © 2013 John Wiley & Sons Ltd.

  11. First report of Tasmanian sheep strain (G2) genotype isolated from Iranian goat using the high resolution melting (HRM) analysis.

    Science.gov (United States)

    Hosseini-Safa, Ahmad; Mohag Hegh, Mohammad Ali; Pestechian, Nader; Ganji, Maryam; Mohammadi, Rasoul; Mahmoudi Lamouki, Reza; Rostami-Nejad, Mohammad

    2016-12-01

    The present study was aimed to evaluate E. granulosus genotypes isolated from goats using HRM analysis in Isfahan province. Cystic echincoccosis, so-called hydatidosis, is widespread infection caused by the larval stage of Echinococcus granulosus . This is an important zoonotic disease worldwide, especially in the developing countries such as Iran. To date, molecular studies mainly based on the mitochondrial DNA sequences have identified distinct genotypes termed G1-G10 which can differ in some characteristics such as the growth and infectivity to different intermediate hosts or the survival rate in the definitive hosts that are important for the development of control strategies. From August to December 2014, 1341 goats were investigated and hydatid cysts were collected from the liver and lungs of 43 infected goats in Isfahan province abattoirs, Isfahan, Iran. Total genomic DNA was extracted from each sample, amplified for the presence of polymorphism of mitochondrial gene coding for cytochrome c oxidase subunit 1 (CO1), using high resolution melting curve (HRM) method. the results of HRM analysis using the sequence of CO1 gene for 43 Echinococcus granulosus isolates from goats showed 31, 2 and 10 isolates were identified as G1, G2, and G3 genotypes, respectively. G1 is the predominant genotype in the isolated goat samples in Isfahan province, and the presence of G2 strain was reported for the first time in goat in Iran.

  12. Applying sociodramatic methods in teaching transition to palliative care.

    Science.gov (United States)

    Baile, Walter F; Walters, Rebecca

    2013-03-01

    We introduce the technique of sociodrama, describe its key components, and illustrate how this simulation method was applied in a workshop format to address the challenge of discussing transition to palliative care. We describe how warm-up exercises prepared 15 learners who provide direct clinical care to patients with cancer for a dramatic portrayal of this dilemma. We then show how small-group brainstorming led to the creation of a challenging scenario wherein highly optimistic family members of a 20-year-old young man with terminal acute lymphocytic leukemia responded to information about the lack of further anticancer treatment with anger and blame toward the staff. We illustrate how the facilitators, using sociodramatic techniques of doubling and role reversal, helped learners to understand and articulate the hidden feelings of fear and loss behind the family's emotional reactions. By modeling effective communication skills, the facilitators demonstrated how key communication skills, such as empathic responses to anger and blame and using "wish" statements, could transform the conversation from one of conflict to one of problem solving with the family. We also describe how we set up practice dyads to give the learners an opportunity to try out new skills with each other. An evaluation of the workshop and similar workshops we conducted is presented. Copyright © 2013 U.S. Cancer Pain Relief Committee. Published by Elsevier Inc. All rights reserved.

  13. Applying multi-resolution numerical methods to geodynamics

    Science.gov (United States)

    Davies, David Rhodri

    Computational models yield inaccurate results if the underlying numerical grid fails to provide the necessary resolution to capture a simulation's important features. For the large-scale problems regularly encountered in geodynamics, inadequate grid resolution is a major concern. The majority of models involve multi-scale dynamics, being characterized by fine-scale upwelling and downwelling activity in a more passive, large-scale background flow. Such configurations, when coupled to the complex geometries involved, present a serious challenge for computational methods. Current techniques are unable to resolve localized features and, hence, such models cannot be solved efficiently. This thesis demonstrates, through a series of papers and closely-coupled appendices, how multi-resolution finite-element methods from the forefront of computational engineering can provide a means to address these issues. The problems examined achieve multi-resolution through one of two methods. In two-dimensions (2-D), automatic, unstructured mesh refinement procedures are utilized. Such methods improve the solution quality of convection dominated problems by adapting the grid automatically around regions of high solution gradient, yielding enhanced resolution of the associated flow features. Thermal and thermo-chemical validation tests illustrate that the technique is robust and highly successful, improving solution accuracy whilst increasing computational efficiency. These points are reinforced when the technique is applied to geophysical simulations of mid-ocean ridge and subduction zone magmatism. To date, successful goal-orientated/error-guided grid adaptation techniques have not been utilized within the field of geodynamics. The work included herein is therefore the first geodynamical application of such methods. In view of the existing three-dimensional (3-D) spherical mantle dynamics codes, which are built upon a quasi-uniform discretization of the sphere and closely coupled

  14. Analytic methods in applied probability in memory of Fridrikh Karpelevich

    CERN Document Server

    Suhov, Yu M

    2002-01-01

    This volume is dedicated to F. I. Karpelevich, an outstanding Russian mathematician who made important contributions to applied probability theory. The book contains original papers focusing on several areas of applied probability and its uses in modern industrial processes, telecommunications, computing, mathematical economics, and finance. It opens with a review of Karpelevich's contributions to applied probability theory and includes a bibliography of his works. Other articles discuss queueing network theory, in particular, in heavy traffic approximation (fluid models). The book is suitable

  15. Reactor calculation in coarse mesh by finite element method applied to matrix response method

    International Nuclear Information System (INIS)

    Nakata, H.

    1982-01-01

    The finite element method is applied to the solution of the modified formulation of the matrix-response method aiming to do reactor calculations in coarse mesh. Good results are obtained with a short running time. The method is applicable to problems where the heterogeneity is predominant and to problems of evolution in coarse meshes where the burnup is variable in one same coarse mesh, making the cross section vary spatially with the evolution. (E.G.) [pt

  16. ITS2 barcoding DNA region combined with high resolution melting (HRM) analysis of Hyoscyami Semen, the mature seed of Hyoscyamus niger.

    Science.gov (United States)

    Xiong, Chao; Hu, Zhi-Gang; Tu, Yuan; Liu, He-Gang; Wang, Ping; Zhao, Ming-Ming; SHIi, Yu-Hua; Wu, Lan; Sun, Wei; Chen, Shi-Lin

    2016-12-01

    Hyoscyami Semen, the mature dried seed of Hyoscyamus niger L., has long been used as a traditional Chinese medicine to treat human diseases. Hyoscyami Semen is found in local markets in China. In markets, sellers and buyers commonly inadvertently mix the seeds of H. niger with the seeds of related species such as Hygrophila salicifolia (Vahl) Nees, Astragalus complanatus R. Br., Cuscuta australis R. Br., Cuscuta chinensis Lam., and Impatiens balsamina L. because of their similar morphologies or similar names. Thus, developing a reliable method for discriminating H. niger seeds from its adulterants is necessary to reduce confusion and ensure the safe use of Hyoscyami Semen. The present study was designed to evaluate the efficiency of high-resolution melting analysis combined with DNA barcoding (Bar-HRM) with internal transcribed spacer 2 to discriminate H. niger. Our results show that Bar-HRM successfully identified the adulterants and detected the proportion of H. niger DNA extract within an admixture. In particular, HRM detected H. niger DNA extract in A. complanatus DNA extract at concentrations as low as 1%. In conclusion, the Bar-HRM method developed in the present study for authenticating H. niger is rapid and cost-effective. It can be used in the future to guarantee the purity of Hyoscyami Semen for the clinical use. Copyright © 2016 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.

  17. Authenticity analyses of Phyllanthus amarus using barcoding coupled with HRM analysis to control its quality for medicinal plant product.

    Science.gov (United States)

    Buddhachat, Kittisak; Osathanunkul, Maslin; Madesis, Panagiotis; Chomdej, Siriwadee; Ongchai, Siriwan

    2015-11-15

    The Phyllanthus genus, a plant used in traditional Thai medicine, has according to several pharmacopeias hepatoprotective properties. Not only is the anatomical morphology of these species relatively similar but they also share the Thai common names Look-Tai-Bai (ลูกใต้ใบ) and Yah-Tai-Bai (หญ้าใต้ใบ), which might cause confusion for laypersons. This study attempted to develop a method for accurate identification of Phyllanthus species, especially Phyllanthus amarus, and to detect contaminants in P. amarus products by using DNA barcoding coupled with high resolution melting (HRM) analysis (bar-HRM). Two plastid loci (rbcL and trnL) were chosen for DNA barcoding to generate a suitable primer for distinguishing Phyllanthus species by HRM analysis. The five species of Phyllanthus were subjected to amplification for testing the specificity and discrimination power of the designed primers derived from rbcL and trnL regions. Sensitivity of the method (DNA barcoding conjugated with HRM) to detect adulterant in P. amarus samples was evaluated. The commercial P. amarus products obtained from a local market were authenticated. The primer pair derived from trnL DNA barcoding (PhylltrnL) had more specificity and power of discrimination for Phyllanthus species than that derived from rbcL DNA barcoding (PhyllrbcL). The result showed that Tm of P. amarus, Phyllanthus urinaria, Phyllanthus debilis, Phyllanthus airy-shawii, and Phyllanthus virgatus was 74.3±0.08, 73.04±0.07, 73.36±0.05, 72.21±0.06, 72.77±0.15°C, respectively. This method proved to be a very sensitive tool that can be used for rapid detection of contamination as low as 1% of other Phyllanthus species in P. amarus admixtures. All commercial products of P. amarus obtained from a local market in Thailand were found to contain pure raw materials of P. amarus without any substitution or contamination. Our results indicated that the use of DNA barcoding coupled with HRM was an

  18. The heterogeneous response method applied to couple the average pin cell and bulk moderator in cluster geometry

    International Nuclear Information System (INIS)

    Lerner, A.M.

    1986-01-01

    The first step towards evaluation of the neutron flux throughout a fuel cluster usually consists of obtaining the multigroup flux distribution in the average pin cell and in the circular outside system of shroud and bulk moderator. Here, an application of the so-called heterogeneous response method (HRM) is described to find this multigroup flux. The rather complex geometry is reduced to a microsystem, the average pin cell, and the outside or macrosystem of shroud and bulk moderator. In each of these systems, collision probabilities are used to obtain their response fluxes caused by sources and in-currents. The two systems are then coupled by cosine currents across that fraction of the average pin-cell boundary, called 'window', that represents the average common boundary between pin cells and the outside system. (author)

  19. Assessment method for analyzing and developing human resource management practices in nuclear power industry

    International Nuclear Information System (INIS)

    Maeki, E.; Pahkin, K.; Lindstroem, S.; Kurki, A-L.

    2014-01-01

    Implementation of HRM practices is often devolved from the HRM unit to front line managers and supervisors. However, the implementation of these practices by line managers and supervisors may vary significantly. They may, for example, be unaware of how to implement HRM practices or skeptical towards the effectiveness of the intended practices. Based on the literature, interviews and workshops in the nuclear power industry, a self-assessment method of HRM practices for intra-organizational use was developed. The assessment method was piloted in four nuclear power organizations. The assessment method seems to be a good tool for generating fruitful discussion on HRM practices, finding areas of HRM practices that need to be developed, and triggering peer-to-peer knowledge sharing and learning on HRM practices. (authors)

  20. Assessment method for analyzing and developing human resource management practices in the nuclear power industry

    International Nuclear Information System (INIS)

    Maeki, Eerikki; Pahkin, Krista; Lindstroem, S.; Kurki, Anna-Leena

    2015-01-01

    Implementation of HRM practices is often devolved from the HRM unit to front line managers and supervisors. However, the implementation of these practices by line managers and supervisors may vary significantly. They may, for example, be unaware of how to implement HRM practices or sceptical towards the effectiveness of the intended practices. Based on the literature, interviews and workshops in the nuclear power industry, a self-assessment method of HRM practices for intra-organizational use was developed. The assessment method was piloted in four nuclear power organizations. The assessment method seems to be a good tool for generating fruitful discussion on HRM practices, finding areas of HRM practices that need to be developed, and triggering peer-to-peer knowledge sharing and learning on HRM practices.

  1. Assessment method for analyzing and developing human resource management practices in the nuclear power industry

    Energy Technology Data Exchange (ETDEWEB)

    Maeki, Eerikki [Aalto Univ. (Finland). Dept. of Industrial Engineering and Management; Pahkin, Krista; Lindstroem, S.; Kurki, Anna-Leena [Finnish Institue of Occupational Health, Helsinki (Finland). Centre of Expertise for the Development of Work and Organizations

    2015-04-15

    Implementation of HRM practices is often devolved from the HRM unit to front line managers and supervisors. However, the implementation of these practices by line managers and supervisors may vary significantly. They may, for example, be unaware of how to implement HRM practices or sceptical towards the effectiveness of the intended practices. Based on the literature, interviews and workshops in the nuclear power industry, a self-assessment method of HRM practices for intra-organizational use was developed. The assessment method was piloted in four nuclear power organizations. The assessment method seems to be a good tool for generating fruitful discussion on HRM practices, finding areas of HRM practices that need to be developed, and triggering peer-to-peer knowledge sharing and learning on HRM practices.

  2. How can schools and teachers benefit from Human Resources Management? Conceptualising HRM from content and process perspectives

    NARCIS (Netherlands)

    Runhaar, P.R.

    2017-01-01

    The necessity for schools to implement human resources management (HRM) is increasingly acknowledged. Specifically, HRM holds the potential of increasing student outcomes through the increased involvement, empowerment and motivation of teachers. In educational literature, however, little empirical

  3. Valuing national effects of digital health investments: an applied method.

    Science.gov (United States)

    Hagens, Simon; Zelmer, Jennifer; Frazer, Cassandra; Gheorghiu, Bobby; Leaver, Chad

    2015-01-01

    This paper describes an approach which has been applied to value national outcomes of investments by federal, provincial and territorial governments, clinicians and healthcare organizations in digital health. Hypotheses are used to develop a model, which is revised and populated based upon the available evidence. Quantitative national estimates and qualitative findings are produced and validated through structured peer review processes. This methodology has applied in four studies since 2008.

  4. Improvements in Sensible Heat-Flux Parametrization in the High-Resolution Regional Model (HRM) Through the Modified Treatment of the Roughness Length for Heat

    Science.gov (United States)

    Anurose, T. J.; Subrahamanyam, D. Bala

    2013-06-01

    We discuss the impact of the differential treatment of the roughness lengths for momentum and heat (z_{0m} and z_{0h}) in the flux parametrization scheme of the high-resolution regional model (HRM) for a heterogeneous terrain centred around Thiruvananthapuram, India (8.5°N, 76.9°E). The magnitudes of sensible heat flux ( H) obtained from HRM simulations using the original parametrization scheme differed drastically from the concurrent in situ observations. With a view to improving the performance of this parametrization scheme, two distinct modifications are incorporated: (1) In the first method, a constant value of 100 is assigned to the z_{0m}/z_{0h} ratio; (2) and in the second approach, this ratio is treated as a function of time. Both these modifications in the HRM model showed significant improvements in the H simulations for Thiruvananthapuram and its adjoining regions. Results obtained from the present study provide a first-ever comparison of H simulations using the modified parametrization scheme in the HRM model with in situ observations for the Indian coastal region, and suggest a differential treatment of z_{0m} and z_{0h} in the flux parametrization scheme.

  5. UGT1A1 (TA)n genotyping in sickle-cell disease: high resolution melting (HRM) curve analysis or direct sequencing, what is the best way?

    Science.gov (United States)

    Thomas, Vincent; Mazard, Blandine; Garcia, Caroline; Lacan, Philippe; Gagnieu, Marie-Claude; Joly, Philippe

    2013-09-23

    Minucci et al. have proposed in 2010 a rapid, simple and cost-effective HRM method on the LightCycler 480® apparatus (Roche) for the determination of the 6/6, 6/7 and 7/7 genotypes of the (TA)n UGT1A1 promoter polymorphism. However, they have not studied the n=5 and n=8 alleles which can be quite frequent in sickle-cell disease patients. The aim of our study was to test this HRM protocol to all the 10 possible (TA)n UGT1A1 genotypes (i.e. 5/5, 5/6, 5/7, 5/8, 6/6, 6/7, 6/8, 7/7, 7/8 and 8/8) by using our SCD cohort of patients. All genotypes could be unambiguously identified except 6/7 and 6/8 which give a similar HRM profile. For those two genotypes, the differentiation necessitates either a direct Sanger sequencing or a second PCR protocol followed by a 3% agarose gel migration. For the (TA)n UGT1A1 promoter genotyping of African patients, each lab has to wonder what is the best way between (i) direct Sanger sequencing of all patients and (ii) HRM protocol for all patients followed by a complementary analysis to differentiate the 6/7 and 6/8 genotypes. © 2013. Published by Elsevier B.V. All rights reserved.

  6. Dose rate reduction method for NMCA applied BWR plants

    International Nuclear Information System (INIS)

    Nagase, Makoto; Aizawa, Motohiro; Ito, Tsuyoshi; Hosokawa, Hideyuki; Varela, Juan; Caine, Thomas

    2012-09-01

    BRAC (BWR Radiation Assessment and Control) dose rate is used as an indicator of the incorporation of activated corrosion by products into BWR recirculation piping, which is known to be a significant contributor to dose rate received by workers during refueling outages. In order to reduce radiation exposure of the workers during the outage, it is desirable to keep BRAC dose rates as low as possible. After HWC was adopted to reduce IGSCC, a BRAC dose rate increase was observed in many plants. As a countermeasure to these rapid dose rate increases under HWC conditions, Zn injection was widely adopted in United States and Europe resulting in a reduction of BRAC dose rates. However, BRAC dose rates in several plants remain high, prompting the industry to continue to investigate methods to achieve further reductions. In recent years a large portion of the BWR fleet has adopted NMCA (NobleChem TM ) to enhance the hydrogen injection effect to suppress SCC. After NMCA, especially OLNC (On-Line NobleChem TM ), BRAC dose rates were observed to decrease. In some OLNC applied BWR plants this reduction was observed year after year to reach a new reduced equilibrium level. This dose rate reduction trends suggest the potential dose reduction might be obtained by the combination of Pt and Zn injection. So, laboratory experiments and in-plant tests were carried out to evaluate the effect of Pt and Zn on Co-60 deposition behaviour. Firstly, laboratory experiments were conducted to study the effect of noble metal deposition on Co deposition on stainless steel surfaces. Polished type 316 stainless steel coupons were prepared and some of them were OLNC treated in the test loop before the Co deposition test. Water chemistry conditions to simulate HWC were as follows: Dissolved oxygen, hydrogen and hydrogen peroxide were below 5 ppb, 100 ppb and 0 ppb (no addition), respectively. Zn was injected to target a concentration of 5 ppb. The test was conducted up to 1500 hours at 553 K. Test

  7. HRM Practices and MNC knowledge Transfer

    DEFF Research Database (Denmark)

    Minbaeva, Dana

    2004-01-01

    and support organizational learning environment, is positively related to the degree ofknowledge transfer to the subsidiary. Moreover, the higher degree of knowledge transfer isexpected when human resource management practices are applied as an integrated system ofinterdependent practices. Hypotheses derived......The purpose of the paper is to determine and empirically examine the effect of human resourcemanagement practices on knowledge transfer within multinational corporations. It is suggested thatthe employment of human resource practices, which affect absorptive capacity of knowledgereceivers...

  8. Beyond the line: exploring the HRM responsibilities of line managers, project managers and the HRM department in four project-oriented companies in the Netherlands, Austria, the UK and the USA

    NARCIS (Netherlands)

    Keegan, A.; Huemann, M.; Turner, J.R.

    2012-01-01

    The topic of what human resource management (HRM) responsibilities are devolved from the HRM department to line managers has attracted much interest in recent years. We report findings from a study on the devolution of HRM practices in four project-oriented companies (POCs) and argue that although

  9. In search of balance – managing the dualities of HRM: an overview of the issues

    NARCIS (Netherlands)

    Boselie, J.P.P.E.F.|info:eu-repo/dai/nl/177012277; Brewster, C.; Paauwe, J.

    2009-01-01

    Purpose – The purpose of this paper is to provide an overview of the human resource management (HRM) literature that builds up to our current concern with dualities, paradoxes, ambiguities, and balance issues; and to introduce the six papers in this special issue on managing the dualities in HRM.

  10. Changes in HRM in Europe: A Longitudinal Comparative Study among 18 European Countries

    Science.gov (United States)

    Nikandrou, Irene; Apospori, Eleni; Papalexandris, Nancy

    2005-01-01

    Purpose: To examine HRM strategies and practices and HRM position within organizations in various cultural, economic and sociopolitical contexts from a longitudinal perspective. Design/methodology/approach: The study uses the 1995 and 1999 Cranet data in a longitudinal methodological framework to explore the changes and trends in 18 European…

  11. Highly Relevant Mentoring (HRM) as a Faculty Development Model for Web-Based Instruction

    Science.gov (United States)

    Carter, Lorraine; Salyers, Vincent; Page, Aroha; Williams, Lynda; Albl, Liz; Hofsink, Clarence

    2012-01-01

    This paper describes a faculty development model called the highly relevant mentoring (HRM) model; the model includes a framework as well as some practical strategies for meeting the professional development needs of faculty who teach web-based courses. The paper further emphasizes the need for faculty and administrative buy-in for HRM and…

  12. How Do Employee Perceptions of HRM Relate to Performance? : A Meta-Analysis of Mediating Mechanisms

    NARCIS (Netherlands)

    Meijerink, Jeroen; Beijer, Susanne; Bos-Nehles, Anna C.

    2017-01-01

    A substantial body of research has examined how employee perceptions of HRM systems relate to employee performance. Although relying on a variety of measures to examine perceived HRM systems, existing studies are unclear to what extent these measures differ in their relationships with important

  13. The effect of HRM on the relationship between quality management techniques and performance

    DEFF Research Database (Denmark)

    do Nascimento Gambi, Lillian; Jørgensen, Frances; Cecílio Gerolamo, Mateus

    2014-01-01

    . The study shows that the relationship between quality management techniques and performance is influenced by HRM approaches, but provides counterevidence to the prevailing assumption that commitment-oriented HRM is generally more appropriate to increase performance than a control-oriented approach. Rather...

  14. E-HRM Usage and Value Creation. Does a Facilitating Context Matter?

    NARCIS (Netherlands)

    Ruel, Hubertus Johannes Maria; van der Kaap, Harm G.

    2012-01-01

    Electronic Human Resource Management (e-HRM) is assumed to be a driving force behind HRM value creation. However, the issue remains of whether empirical evidence supports this assumption. Moreover, is the relationship straightforward and direct or is it conditional, and do contextual factors

  15. The Impact of Organized Violence and Crime on HRM and Work Practices

    DEFF Research Database (Denmark)

    Ramirez, Jacobo; Madero, Sergio

    of organized violence and crime on HRM and work practices. The results made it possible to observe a complex configuration between HRM policies and practices and managerial style, in the context of organized crime and violence in Mexico. A combination of strict employees’ control, emphasis on soft...

  16. How strategic considerations influence decision making on e-HRM applications

    NARCIS (Netherlands)

    Schalk, R.; Timmerman, V.; van den Heuvel, S.R.H.

    2013-01-01

    Although numerous studies indicate that the added value of human resource management is strongest when HRM decisions are linked to the organizational strategy, practical knowledge about how strategic considerations influence decision-making processes relating to e-HRM is limited. Therefore, the

  17. Exploring the Best HRM Practices-Performance Relationship: An Empirical Approach

    Science.gov (United States)

    Theriou, George N.; Chatzoglou, Prodromos D.

    2009-01-01

    Purpose: This paper aims to empirically examine the linkages between best human resource (HRM) practices, knowledge management, organisational learning, organisational capabilities and organisational performance. The proposed framework and findings intend to add to the understanding of the specific processes that mediate between best HRM practices…

  18. Coordinated vs. liberal market HRM: the impact of institutionalisation on multinational firms

    NARCIS (Netherlands)

    Farndale, E.; Brewster, C.; Poutsma, F.

    2008-01-01

    The impact of institutionalized contexts on the HRM activities of multinational firms has become a focus of increasing attention in recent literature. However, theories of how different types of business systems or market economies may influence HRM, and the impact of context on multinational

  19. Implementing Human Resources Management (HRM) within Dutch VET Institutions: Examining the Fostering and Hindering Factors

    Science.gov (United States)

    Runhaar, Piety; Sanders, Karin

    2013-01-01

    Vocational Education and Training (VET) Institutions face serious challenges, like the implementation of competence-based education and upcoming teacher shortages, which urge them to implement Human Resources Management policy and practices (HRM). The implementation of HRM, however, often stagnates. This paper describes a qualitative study--in…

  20. Franchised and Small, the Most Beautiful of All; HRM and Performance in Plural Systems

    NARCIS (Netherlands)

    Brand, M.J.; Croonen, E.P.M.

    2010-01-01

    Franchising is particularly present in industries in which human resources play a vital role. However, little is known about human resource management (HRM) in a franchising context. This study investigates HRM behavior and performance using a sample of 171 units within one plural retailing system.

  1. The harmonics detection method based on neural network applied ...

    African Journals Online (AJOL)

    Several different methods have been used to sense load currents and extract its ... in order to produce a reference current in shunt active power filters (SAPF), and ... technique compared to other similar methods are found quite satisfactory by ...

  2. Muon radiography method for fundamental and applied research

    Science.gov (United States)

    Alexandrov, A. B.; Vladymyrov, M. S.; Galkin, V. I.; Goncharova, L. A.; Grachev, V. M.; Vasina, S. G.; Konovalova, N. S.; Malovichko, A. A.; Managadze, A. K.; Okat'eva, N. M.; Polukhina, N. G.; Roganova, T. M.; Starkov, N. I.; Tioukov, V. E.; Chernyavsky, M. M.; Shchedrina, T. V.

    2017-12-01

    This paper focuses on the basic principles of the muon radiography method, reviews the major muon radiography experiments, and presents the first results in Russia obtained by the authors using this method based on emulsion track detectors.

  3. Methodical Aspects of Applying Strategy Map in an Organization

    OpenAIRE

    Piotr Markiewicz

    2013-01-01

    One of important aspects of strategic management is the instrumental aspect included in a rich set of methods and techniques used at particular stages of strategic management process. The object of interest in this study is the development of views and the implementation of strategy as an element of strategic management and instruments in the form of methods and techniques. The commonly used method in strategy implementation and measuring progress is Balanced Scorecard (BSC). The method was c...

  4. Classical and modular methods applied to Diophantine equations

    NARCIS (Netherlands)

    Dahmen, S.R.

    2008-01-01

    Deep methods from the theory of elliptic curves and modular forms have been used to prove Fermat's last theorem and solve other Diophantine equations. These so-called modular methods can often benefit from information obtained by other, classical, methods from number theory; and vice versa. In our

  5. How do developmental and accommodative HRM enhance employee engagement and commitment? : The role of psychological contract and SOC-strategies

    NARCIS (Netherlands)

    Bal, P.M.; Kooij, T.A.M.; de Jong, S.B.

    2013-01-01

    In the context of the changing workforce, this study introduced two perspectives on HRM and distinguished universalistic developmental HRM from contingent accommodative HRM. We predicted two separate pathways for the effects on two employee outcomes: work engagement and affective commitment. We

  6. Human resource practices and organisational performance: Can the HRM-performance linkage be explained by the cooperative behaviours of employees?

    NARCIS (Netherlands)

    Lambooij, Mattijs; Sanders, Karin; Koster, Ferry; Zwiers, Marieke

    2006-01-01

    This paper addresses the question as to whether the linkage between HRM and organisational performance can be explained by the effect of the internal and strategic fit of HRM on the cooperative behaviours of employees. We expect that the more HRM practices are aligned within themselves (internal

  7. E-HRM: innovation or irritation? An exploration of web-based human resource management in large companies

    NARCIS (Netherlands)

    Ruel, Hubertus Johannes Maria; Bondarouk, Tatiana; Looise, Jan C.

    2004-01-01

    Human Resource Management (HRM of HR) executives are feeling pressured by the threat of a new development in recent years: Electronic Human Resource Management systems, also known as E-HRM. Consultants and other experts purport that this phenomenon will decrease the number of HRM employees, reduce

  8. The pseudo-harmonics method applied to depletion calculation

    International Nuclear Information System (INIS)

    Silva, F.C. da; Amaral, J.A.C.; Thome, Z.D.

    1989-01-01

    In this paper, a new method for performing depletion calculations, based on the use of the Pseudo-Harmonics perturbation method, was developed. The fuel burnup was considered as a global perturbation and the multigroup difusion equations were rewriten in such a way as to treat the soluble boron concentration as the eigenvalue. By doing this, the critical boron concentration can be obtained by a perturbation method. A test of the new method was performed for a H 2 O-colled, D 2 O-moderated reactor. Comparison with direct calculation showed that this method is very accurate and efficient. (author) [pt

  9. HRM, POWER and possible spaces of becoming human

    DEFF Research Database (Denmark)

    Bramming, Pia

    2003-01-01

    What has power to do with Human Resource Management (HRM)? Perusing HRMtextbooksone will find, that power as a concept, only seldom is approached explicitly.When the subject of power is addressed directly, it is primarily as a question ofbargaining power between organisation and labour market...... institutions, the power of aleader or person in terms of the right to execute punishment and the duty to obedienceor empowerment, as a countermove to the effects of bureaucratic workplace routines`... where initiative is stifled and workers become alienated'1. Indirectly one canidentify power as interesting...... through technologies of theself....

  10. Genotyping of Listeria monocytogenes isolates from poultry carcasses using high resolution melting (HRM) analysis.

    Science.gov (United States)

    Sakaridis, Ioannis; Ganopoulos, Ioannis; Madesis, Panagiotis; Tsaftaris, Athanasios; Argiriou, Anagnostis

    2014-01-02

    An outbreak situation of human listeriosis requires a fast and accurate protocol for typing Listeria monocytogenes . Existing techniques are either characterized by low discriminatory power or are laborious and require several days to give a final result. Polymerase chain reaction (PCR) coupled with high resolution melting (HRM) analysis was investigated in this study as an alternative tool for a rapid and precise genotyping of L. monocytogenes isolates. Fifty-five isolates of L. monocytogenes isolated from poultry carcasses and the environment of four slaughterhouses were typed by HRM analysis using two specific markers, internalin B and ssrA genes. The analysis of genotype confidence percentage of L. monocytogenes isolates produced by HRM analysis generated dendrograms with two major groups and several subgroups. Furthermore, the analysis of the HRM curves revealed that all L. monocytogenes isolates could easily be distinguished. In conclusion, HRM was proven to be a fast and powerful tool for genotyping isolates of L. monocytogenes .

  11. Quality assessment of DNA derived from up to 30 years old formalin fixed paraffin embedded (FFPE) tissue for PCR-based methylation analysis using SMART-MSP and MS-HRM.

    Science.gov (United States)

    Kristensen, Lasse S; Wojdacz, Tomasz K; Thestrup, Britta B; Wiuf, Carsten; Hager, Henrik; Hansen, Lise Lotte

    2009-12-21

    The High Resolution Melting (HRM) technology has recently been introduced as a rapid and robust analysis tool for the detection of DNA methylation. The methylation status of multiple tumor suppressor genes may serve as biomarkers for early cancer diagnostics, for prediction of prognosis and for prediction of response to treatment. Therefore, it is important that methodologies for detection of DNA methylation continue to evolve. Sensitive Melting Analysis after Real Time - Methylation Specific PCR (SMART-MSP) and Methylation Sensitive - High Resolution Melting (MS-HRM) are two methods for single locus DNA methylation detection based on HRM. Here, we have assessed the quality of DNA extracted from up to 30 years old Formalin Fixed Paraffin Embedded (FFPE) tissue for DNA methylation analysis using SMART-MSP and MS-HRM. The quality assessment was performed on DNA extracted from 54 Non-Small Cell Lung Cancer (NSCLC) samples derived from FFPE tissue, collected over 30 years and grouped into five years intervals. For each sample, the methylation levels of the CDKN2A (p16) and RARB promoters were estimated using SMART-MSP and MS-HRM assays designed to assess the methylation status of the same CpG positions. This allowed for a direct comparison of the methylation levels estimated by the two methods for each sample. CDKN2A promoter methylation levels were successfully determined by SMART-MSP and MS-HRM in all 54 samples. Identical methylation estimates were obtained by the two methods in 46 of the samples. The methylation levels of the RARB promoter were successfully determined by SMART-MSP in all samples. When using MS-HRM to assess RARB methylation five samples failed to amplify and 15 samples showed a melting profile characteristic for heterogeneous methylation. Twenty-seven of the remaining 34 samples, for which the methylation level could be estimated, gave the same result as observed when using SMART-MSP. MS-HRM and SMART-MSP can be successfully used for single locus

  12. Waste classification and methods applied to specific disposal sites

    International Nuclear Information System (INIS)

    Rogers, V.C.

    1979-01-01

    An adequate definition of the classes of radioactive wastes is necessary to regulating the disposal of radioactive wastes. A classification system is proposed in which wastes are classified according to characteristics relating to their disposal. Several specific sites are analyzed with the methodology in order to gain insights into the classification of radioactive wastes. Also presented is the analysis of ocean dumping as it applies to waste classification. 5 refs

  13. nuclear and atomic methods applied in the determination of some

    African Journals Online (AJOL)

    NAA is a quantitative and qualitative method for the precise determination of a number of major, minor and trace elements in different types of geological, environmental and biological samples. It is based on nuclear reaction between neutron and target nuclei of a sample material. It is a useful method for the simultaneous.

  14. Instructions for applying inverse method for reactivity measurement

    International Nuclear Information System (INIS)

    Milosevic, M.

    1988-11-01

    This report is a brief description of the completed method for reactivity measurement. It contains description of the experimental procedure needed instrumentation and computer code IM for determining reactivity. The objective of this instructions manual is to enable experiments and reactivity measurement on any critical system according to the methods adopted at the RB reactor

  15. The spectral volume method as applied to transport problems

    International Nuclear Information System (INIS)

    McClarren, Ryan G.

    2011-01-01

    We present a new spatial discretization for transport problems: the spectral volume method. This method, rst developed by Wang for computational fluid dynamics, divides each computational cell into several sub-cells and enforces particle balance on each of these sub-cells. Also, these sub-cells are used to build a polynomial reconstruction in the cell. The idea of dividing cells into many cells is a generalization of the simple corner balance and other similar schemes. The spectral volume method preserves particle conservation and preserves the asymptotic diffusion limit. We present results from the method on two transport problems in slab geometry using discrete ordinates and second through sixth order spectral volume schemes. The numerical results demonstrate the accuracy and preservation of the diffusion limit of the spectral volume method. Future work will explore possible bene ts of the scheme for high-performance computing and for resolving diffusive boundary layers. (author)

  16. Literature Review of Applying Visual Method to Understand Mathematics

    Directory of Open Access Journals (Sweden)

    Yu Xiaojuan

    2015-01-01

    Full Text Available As a new method to understand mathematics, visualization offers a new way of understanding mathematical principles and phenomena via image thinking and geometric explanation. It aims to deepen the understanding of the nature of concepts or phenomena and enhance the cognitive ability of learners. This paper collates and summarizes the application of this visual method in the understanding of mathematics. It also makes a literature review of the existing research, especially with a visual demonstration of Euler’s formula, introduces the application of this method in solving relevant mathematical problems, and points out the differences and similarities between the visualization method and the numerical-graphic combination method, as well as matters needing attention for its application.

  17. Methodical Aspects of Applying Strategy Map in an Organization

    Directory of Open Access Journals (Sweden)

    Piotr Markiewicz

    2013-06-01

    Full Text Available One of important aspects of strategic management is the instrumental aspect included in a rich set of methods and techniques used at particular stages of strategic management process. The object of interest in this study is the development of views and the implementation of strategy as an element of strategic management and instruments in the form of methods and techniques. The commonly used method in strategy implementation and measuring progress is Balanced Scorecard (BSC. The method was created as a result of implementing the project “Measuring performance in the Organization of the future” of 1990, completed by a team under the supervision of David Norton (Kaplan, Norton 2002. The developed method was used first of all to evaluate performance by decomposition of a strategy into four perspectives and identification of measures of achievement. In the middle of 1990s the method was improved by enriching it, first of all, with a strategy map, in which the process of transition of intangible assets into tangible financial effects is reflected (Kaplan, Norton 2001. Strategy map enables illustration of cause and effect relationship between processes in all four perspectives and performance indicators at the level of organization. The purpose of the study being prepared is to present methodical conditions of using strategy maps in the strategy implementation process in organizations of different nature.

  18. Applying a life cycle approach to project management methods

    OpenAIRE

    Biggins, David; Trollsund, F.; Høiby, A.L.

    2016-01-01

    Project management is increasingly important to organisations because projects are the method\\ud by which organisations respond to their environment. A key element within project management\\ud is the standards and methods that are used to control and conduct projects, collectively known as\\ud project management methods (PMMs) and exemplified by PRINCE2, the Project Management\\ud Institute’s and the Association for Project Management’s Bodies of Knowledge (PMBOK and\\ud APMBOK. The purpose of t...

  19. Method for curing alkyd resin compositions by applying ionizing radiation

    International Nuclear Information System (INIS)

    Watanabe, T.; Murata, K.; Maruyama, T.

    1975-01-01

    An alkyd resin composition is prepared by dissolving a polymerizable alkyd resin having from 10 to 50 percent of oil length into a vinyl monomer. The polymerizable alkyd resin is obtained by a half-esterification reaction of an acid anhydride having a polymerizable unsaturated group and an alkyd resin modified with conjugated unsaturated oil having at least one reactive hydroxyl group per one molecule. The alkyd resin composition thus obtained is coated on an article, and ionizing radiation is applied on the article to cure the coated film thereon. (U.S.)

  20. The integral equation method applied to eddy currents

    International Nuclear Information System (INIS)

    Biddlecombe, C.S.; Collie, C.J.; Simkin, J.; Trowbridge, C.W.

    1976-04-01

    An algorithm for the numerical solution of eddy current problems is described, based on the direct solution of the integral equation for the potentials. In this method only the conducting and iron regions need to be divided into elements, and there are no boundary conditions. Results from two computer programs using this method for iron free problems for various two-dimensional geometries are presented and compared with analytic solutions. (author)

  1. Electronic Human Resources Management (e-HRM Adoption Studies: Past and Future Research

    Directory of Open Access Journals (Sweden)

    Winarto Winarto

    2018-05-01

    Full Text Available Electronic human resource management (e-HRM systems become more widely used by profit and non-profit organization. However, the field currently lacks sound theoretical frameworks that can be useful in addressing a key issue concerning the implementation of e-HRM systems, in particular to obtain a better understanding of the factors influencing the adoption of e-HRM systems. The objective of this paper is to provide a foundation towards the development of a theoretical framework for the implementation of e-HRM systems and develop a conceptual model that would reflect the nature of e-HRM systems’ adoption through systematic literature review. Adopting Crossan and Apaydin’s procedure of systematic review, this paper investigated 21 empirical papers of electronics human resources management, then categorized them into 4 characteristics which influence the adoption; System and technology characteristics; Organizational characteristics; User/individual characteristics, and Environmental and contextual characteristics. Finally, the e-HRM adoption research framework is drawn and based on the framework; avenues for future research are discussed.   Bahasa Indonesia Abstrak: Manajemen sumber daya manusia elektronik (selanjutnya disebut dengan e-HRM semakin banyak digunakan oleh organisasi profit dan nonprofit. Namun, bidang dan topik ini belum memiliki kerangka teori yang mapan, yang dapat digunakan untuk menganalisis isu-isu terkait penerapan e-HRM, terutama mengenai faktor-faktor yang mempengaruhi adopsi sistem e-HRM. Tujuan penelitian ini adalah untuk memberikan landasan bagi pengembangan kerangka teoritis untuk implementasi sistem e-HRM dan mengembangkan model konseptual yang akan menggambarkan adopsi sistem e-HRM melalui tinjauan literatur sistematis. Mengadopsi prosedur dan metode Crossan dan Apaydin untuk melakukan telaah literatur secara sistematis, paper ini menyelidiki 21 publikasi empiris manajemen sumber daya manusia elektronik dari 2

  2. Apply of torque method at rationalization of work

    Directory of Open Access Journals (Sweden)

    Bandurová Miriam

    2001-03-01

    Full Text Available Aim of the study was to analyse consumption of time for profession - cylinder grinder, by torque method.Method of torque following is used for detection of sorts and size of time slope, on detection of portion of individual sorts of time consumption and cause of time slope. By this way it is possible to find out coefficient of employment and recovery of workers in organizational unit. Advantage of torque survey is low costs on informations acquirement, non-fastidiousness per worker and observer, which is easy trained. It is mentally acceptable method for objects of survey.Finding and detection of reserves in activity of cylinders grinder result of torque was surveys. Loss of time presents till 8% of working time. In 5 - shift service and average occupiying of shift by 4,4 grinder ( from statistic information of service , loss at grinder of cylinders are for whole centre 1,48 worker.According presented information it was recommended to cancel one job place - grinder of cylinders - and reduce state about one grinder. Next job place isn't possible cancel, because grindery of cylinders must to adapt to the grind line by number of polished cylinders in shift and semi - finishing of polished cylinders can not be high for often changes in area of grinding and sortiment changes.By this contribution we confirmed convenience of exploitation of torque method as one of the methods using during the job rationalization.

  3. Thermoluminescence as a dating method applied to the Morocco Neolithic

    International Nuclear Information System (INIS)

    Ousmoi, M.

    1989-09-01

    Thermoluminescence is an absolute dating method which is well adapted to the study of burnt clays and so of the prehistoric ceramics belonging to the Neolithic period. The purpose of this study is to establish a first absolute chronology of the septentrional morocco Neolithic between 3000 and 7000 years before us and some improvements of the TL dating. The first part of the thesis contains some hypothesis about the morocco Neolithic and some problems to solve. Then we study the TL dating method along with new process to ameliorate the quality of the results like the shift of quartz TL peaks or the crushing of samples. The methods which were employed using 24 samples belonging to various civilisations are: the quartz inclusion method and the fine grain technique. For the dosimetry, several methods were used: determination of the K 2 O contents, alpha counting, site dosimetry using TL dosimeters and a scintillation counter. The results which were found bring some interesting answers to the archeologic question and ameliorate the chronologic schema of the Northern morocco Neolithic: development of the old cardial Neolithic in the North, and perhaps in the center of Morocco (the region of Rabat), between 5500 and 7000 before us. Development of the recent middle Neolithic around 4000-5000 before us, with a protocampaniforme (Skhirat), little older than the campaniforme recognized in the south of Spain. Development of the bronze age around 2000-4000 before us [fr

  4. Accuracy of the Garmin 920 XT HRM to perform HRV analysis.

    Science.gov (United States)

    Cassirame, Johan; Vanhaesebrouck, Romain; Chevrolat, Simon; Mourot, Laurent

    2017-12-01

    Heart rate variability (HRV) analysis is widely used to investigate autonomous cardiac drive. This method requires periodogram measurement, which can be obtained by an electrocardiogram (ECG) or from a heart rate monitor (HRM), e.g. the Garmin 920 XT device. The purpose of this investigation was to assess the accuracy of RR time series measurements from a Garmin 920 XT HRM as compared to a standard ECG, and to verify whether the measurements thus obtained are suitable for HRV analysis. RR time series were collected simultaneously with an ECG (Powerlab system, AD Instruments, Castell Hill, Australia) and a Garmin XT 920 in 11 healthy subjects during three conditions, namely in the supine position, the standing position and during moderate exercise. In a first step, we compared RR time series obtained with both tools using the Bland and Altman method to obtain the limits of agreement in all three conditions. In a second step, we compared the results of HRV analysis between the ECG RR time series and Garmin 920 XT series. Results show that the accuracy of this system is in accordance with the literature in terms of the limits of agreement. In the supine position, bias was 0.01, - 2.24, + 2.26 ms; in the standing position, - 0.01, - 3.12, + 3.11 ms respectively, and during exercise, - 0.01, - 4.43 and + 4.40 ms. Regarding HRV analysis, we did not find any difference for HRV analysis in the supine position, but the standing and exercise conditions both showed small modifications.

  5. Modal method for crack identification applied to reactor recirculation pump

    International Nuclear Information System (INIS)

    Miller, W.H.; Brook, R.

    1991-01-01

    Nuclear reactors have been operating and producing useful electricity for many years. Within the last few years, several plants have found cracks in the reactor coolant pump shaft near the thermal barrier. The modal method and results described herein show the analytical results of using a Modal Analysis test method to determine the presence, size, and location of a shaft crack. The authors have previously demonstrated that the test method can analytically and experimentally identify shaft cracks as small as five percent (5%) of the shaft diameter. Due to small differences in material property distribution, the attempt to identify cracks smaller than 3% of the shaft diameter has been shown to be impractical. The rotor dynamics model includes a detailed motor rotor, external weights and inertias, and realistic total support stiffness. Results of the rotor dynamics model have been verified through a comparison with on-site vibration test data

  6. Boron autoradiography method applied to the study of steels

    International Nuclear Information System (INIS)

    Gugelmeier, R.; Barcelo, G.N.; Boado, J.H.; Fernandez, C.

    1986-01-01

    The boron state, contained in the steel microestructure, is determined. The autoradiography by neutrons is used, permiting to obtain boron distribution images by means of additional information which is difficult to acquire by other methods. The application of the method is described, based on the neutronic irradiation of a polished steel sample, over which a celulose nitrate sheet or other appropriate material is fixed to constitute the detector. The particles generated by the neutron-boron interaction affect the detector sheet, which is subsequently revealed with a chemical treatment and can be observed at the optical microscope. In the case of materials used for the construction of nuclear reactors, special attention must be given to the presence of boron, since owing to the exceptionaly high capacity of neutron absorption, lowest quantities of boron acquire importance. The adaption of the method to metallurgical problems allows the obtainment of a correlation between the boron distribution images and the material's microstructure. (M.E.L.) [es

  7. Nonstandard Finite Difference Method Applied to a Linear Pharmacokinetics Model

    Directory of Open Access Journals (Sweden)

    Oluwaseun Egbelowo

    2017-05-01

    Full Text Available We extend the nonstandard finite difference method of solution to the study of pharmacokinetic–pharmacodynamic models. Pharmacokinetic (PK models are commonly used to predict drug concentrations that drive controlled intravenous (I.V. transfers (or infusion and oral transfers while pharmacokinetic and pharmacodynamic (PD interaction models are used to provide predictions of drug concentrations affecting the response of these clinical drugs. We structure a nonstandard finite difference (NSFD scheme for the relevant system of equations which models this pharamcokinetic process. We compare the results obtained to standard methods. The scheme is dynamically consistent and reliable in replicating complex dynamic properties of the relevant continuous models for varying step sizes. This study provides assistance in understanding the long-term behavior of the drug in the system, and validation of the efficiency of the nonstandard finite difference scheme as the method of choice.

  8. Applying Nyquist's method for stability determination to solar wind observations

    Science.gov (United States)

    Klein, Kristopher G.; Kasper, Justin C.; Korreck, K. E.; Stevens, Michael L.

    2017-10-01

    The role instabilities play in governing the evolution of solar and astrophysical plasmas is a matter of considerable scientific interest. The large number of sources of free energy accessible to such nearly collisionless plasmas makes general modeling of unstable behavior, accounting for the temperatures, densities, anisotropies, and relative drifts of a large number of populations, analytically difficult. We therefore seek a general method of stability determination that may be automated for future analysis of solar wind observations. This work describes an efficient application of the Nyquist instability method to the Vlasov dispersion relation appropriate for hot, collisionless, magnetized plasmas, including the solar wind. The algorithm recovers the familiar proton temperature anisotropy instabilities, as well as instabilities that had been previously identified using fits extracted from in situ observations in Gary et al. (2016). Future proposed applications of this method are discussed.

  9. Efficient electronic structure methods applied to metal nanoparticles

    DEFF Research Database (Denmark)

    Larsen, Ask Hjorth

    of efficient approaches to density functional theory and the application of these methods to metal nanoparticles. We describe the formalism and implementation of localized atom-centered basis sets within the projector augmented wave method. Basis sets allow for a dramatic increase in performance compared....... The basis set method is used to study the electronic effects for the contiguous range of clusters up to several hundred atoms. The s-electrons hybridize to form electronic shells consistent with the jellium model, leading to electronic magic numbers for clusters with full shells. Large electronic gaps...... and jumps in Fermi level near magic numbers can lead to alkali-like or halogen-like behaviour when main-group atoms adsorb onto gold clusters. A non-self-consistent NewnsAnderson model is used to more closely study the chemisorption of main-group atoms on magic-number Au clusters. The behaviour at magic...

  10. Variance reduction methods applied to deep-penetration problems

    International Nuclear Information System (INIS)

    Cramer, S.N.

    1984-01-01

    All deep-penetration Monte Carlo calculations require variance reduction methods. Before beginning with a detailed approach to these methods, several general comments concerning deep-penetration calculations by Monte Carlo, the associated variance reduction, and the similarities and differences of these with regard to non-deep-penetration problems will be addressed. The experienced practitioner of Monte Carlo methods will easily find exceptions to any of these generalities, but it is felt that these comments will aid the novice in understanding some of the basic ideas and nomenclature. Also, from a practical point of view, the discussions and developments presented are oriented toward use of the computer codes which are presented in segments of this Monte Carlo course

  11. Non-perturbative methods applied to multiphoton ionization

    International Nuclear Information System (INIS)

    Brandi, H.S.; Davidovich, L.; Zagury, N.

    1982-09-01

    The use of non-perturbative methods in the treatment of atomic ionization is discussed. Particular attention is given to schemes of the type proposed by Keldysh where multiphoton ionization and tunnel auto-ionization occur for high intensity fields. These methods are shown to correspond to a certain type of expansion of the T-matrix in the intra-atomic potential; in this manner a criterium concerning the range of application of these non-perturbative schemes is suggested. A brief comparison between the ionization rate of atoms in the presence of linearly and circularly polarized light is presented. (Author) [pt

  12. On second quantization methods applied to classical statistical mechanics

    International Nuclear Information System (INIS)

    Matos Neto, A.; Vianna, J.D.M.

    1984-01-01

    A method of expressing statistical classical results in terms of mathematical entities usually associated to quantum field theoretical treatment of many particle systems (Fock space, commutators, field operators, state vector) is discussed. It is developed a linear response theory using the 'second quantized' Liouville equation introduced by Schonberg. The relationship of this method to that of Prigogine et al. is briefly analyzed. The chain of equations and the spectral representations for the new classical Green's functions are presented. Generalized operators defined on Fock space are discussed. It is shown that the correlation functions can be obtained from Green's functions defined with generalized operators. (Author) [pt

  13. Review of PCMS and heat transfer enhancement methods applied ...

    African Journals Online (AJOL)

    Most available PCMs have low thermal conductivity making heat transfer enhancement necessary for power applications. The various methods of heat transfer enhancement in latent heat storage systems were also reviewed systematically. The review showed that three commercially - available PCMs are suitable in the ...

  14. E-LEARNING METHOD APPLIED TO TECHNICAL GRAPHICS SUBJECTS

    Directory of Open Access Journals (Sweden)

    GOANTA Adrian Mihai

    2011-11-01

    Full Text Available The paper presents some of the author’s endeavors in creating video courses for the students from the Faculty of Engineering in Braila related to subjects involving technical graphics . There are also mentioned the steps taken in completing the method and how to achieve a feedback on the rate of access to these types of courses by the students.

  15. Current Human Reliability Analysis Methods Applied to Computerized Procedures

    Energy Technology Data Exchange (ETDEWEB)

    Ronald L. Boring

    2012-06-01

    Computerized procedures (CPs) are an emerging technology within nuclear power plant control rooms. While CPs have been implemented internationally in advanced control rooms, to date no US nuclear power plant has implemented CPs in its main control room (Fink et al., 2009). Yet, CPs are a reality of new plant builds and are an area of considerable interest to existing plants, which see advantages in terms of enhanced ease of use and easier records management by omitting the need for updating hardcopy procedures. The overall intent of this paper is to provide a characterization of human reliability analysis (HRA) issues for computerized procedures. It is beyond the scope of this document to propose a new HRA approach or to recommend specific methods or refinements to those methods. Rather, this paper serves as a review of current HRA as it may be used for the analysis and review of computerized procedures.

  16. Probabilist methods applied to electric source problems in nuclear safety

    International Nuclear Information System (INIS)

    Carnino, A.; Llory, M.

    1979-01-01

    Nuclear Safety has frequently been asked to quantify safety margins and evaluate the hazard. In order to do so, the probabilist methods have proved to be the most promising. Without completely replacing determinist safety, they are now commonly used at the reliability or availability stages of systems as well as for determining the likely accidental sequences. In this paper an application linked to the problem of electric sources is described, whilst at the same time indicating the methods used. This is the calculation of the probable loss of all the electric sources of a pressurized water nuclear power station, the evaluation of the reliability of diesels by event trees of failures and the determination of accidental sequences which could be brought about by the 'total electric source loss' initiator and affect the installation or the environment [fr

  17. Theoretical and applied aerodynamics and related numerical methods

    CERN Document Server

    Chattot, J J

    2015-01-01

    This book covers classical and modern aerodynamics, theories and related numerical methods, for senior and first-year graduate engineering students, including: -The classical potential (incompressible) flow theories for low speed aerodynamics of thin airfoils and high and low aspect ratio wings. - The linearized theories for compressible subsonic and supersonic aerodynamics. - The nonlinear transonic small disturbance potential flow theory, including supercritical wing sections, the extended transonic area rule with lift effect, transonic lifting line and swept or oblique wings to minimize wave drag. Unsteady flow is also briefly discussed. Numerical simulations based on relaxation mixed-finite difference methods are presented and explained. - Boundary layer theory for all Mach number regimes and viscous/inviscid interaction procedures used in practical aerodynamics calculations. There are also four chapters covering special topics, including wind turbines and propellers, airplane design, flow analogies and h...

  18. Applying probabilistic methods for assessments and calculations for accident prevention

    International Nuclear Information System (INIS)

    Anon.

    1984-01-01

    The guidelines for the prevention of accidents require plant design-specific and radioecological calculations to be made in order to show that maximum acceptable expsoure values will not be exceeded in case of an accident. For this purpose, main parameters affecting the accident scenario have to be determined by probabilistic methods. This offers the advantage that parameters can be quantified on the basis of unambigious and realistic criteria, and final results can be defined in terms of conservativity. (DG) [de

  19. Applying flow chemistry: methods, materials, and multistep synthesis.

    Science.gov (United States)

    McQuade, D Tyler; Seeberger, Peter H

    2013-07-05

    The synthesis of complex molecules requires control over both chemical reactivity and reaction conditions. While reactivity drives the majority of chemical discovery, advances in reaction condition control have accelerated method development/discovery. Recent tools include automated synthesizers and flow reactors. In this Synopsis, we describe how flow reactors have enabled chemical advances in our groups in the areas of single-stage reactions, materials synthesis, and multistep reactions. In each section, we detail the lessons learned and propose future directions.

  20. Data Mining Methods Applied to Flight Operations Quality Assurance Data: A Comparison to Standard Statistical Methods

    Science.gov (United States)

    Stolzer, Alan J.; Halford, Carl

    2007-01-01

    In a previous study, multiple regression techniques were applied to Flight Operations Quality Assurance-derived data to develop parsimonious model(s) for fuel consumption on the Boeing 757 airplane. The present study examined several data mining algorithms, including neural networks, on the fuel consumption problem and compared them to the multiple regression results obtained earlier. Using regression methods, parsimonious models were obtained that explained approximately 85% of the variation in fuel flow. In general data mining methods were more effective in predicting fuel consumption. Classification and Regression Tree methods reported correlation coefficients of .91 to .92, and General Linear Models and Multilayer Perceptron neural networks reported correlation coefficients of about .99. These data mining models show great promise for use in further examining large FOQA databases for operational and safety improvements.

  1. The colour analysis method applied to homogeneous rocks

    Directory of Open Access Journals (Sweden)

    Halász Amadé

    2015-12-01

    Full Text Available Computer-aided colour analysis can facilitate cyclostratigraphic studies. Here we report on a case study involving the development of a digital colour analysis method for examination of the Boda Claystone Formation which is the most suitable in Hungary for the disposal of high-level radioactive waste. Rock type colours are reddish brown or brownish red, or any shade between brown and red. The method presented here could be used to differentiate similar colours and to identify gradual transitions between these; the latter are of great importance in a cyclostratigraphic analysis of the succession. Geophysical well-logging has demonstrated the existence of characteristic cyclic units, as detected by colour and natural gamma. Based on our research, colour, natural gamma and lithology correlate well. For core Ib-4, these features reveal the presence of orderly cycles with thicknesses of roughly 0.64 to 13 metres. Once the core has been scanned, this is a time- and cost-effective method.

  2. Comparison Study of Subspace Identification Methods Applied to Flexible Structures

    Science.gov (United States)

    Abdelghani, M.; Verhaegen, M.; Van Overschee, P.; De Moor, B.

    1998-09-01

    In the past few years, various time domain methods for identifying dynamic models of mechanical structures from modal experimental data have appeared. Much attention has been given recently to so-called subspace methods for identifying state space models. This paper presents a detailed comparison study of these subspace identification methods: the eigensystem realisation algorithm with observer/Kalman filter Markov parameters computed from input/output data (ERA/OM), the robust version of the numerical algorithm for subspace system identification (N4SID), and a refined version of the past outputs scheme of the multiple-output error state space (MOESP) family of algorithms. The comparison is performed by simulating experimental data using the five mode reduced model of the NASA Mini-Mast structure. The general conclusion is that for the case of white noise excitations as well as coloured noise excitations, the N4SID/MOESP algorithms perform equally well but give better results (improved transfer function estimates, improved estimates of the output) compared to the ERA/OM algorithm. The key computational step in the three algorithms is the approximation of the extended observability matrix of the system to be identified, for N4SID/MOESP, or of the observer for the system to be identified, for the ERA/OM. Furthermore, the three algorithms only require the specification of one dimensioning parameter.

  3. Applying Hierarchical Task Analysis Method to Discovery Layer Evaluation

    Directory of Open Access Journals (Sweden)

    Marlen Promann

    2015-03-01

    Full Text Available Libraries are implementing discovery layers to offer better user experiences. While usability tests have been helpful in evaluating the success or failure of implementing discovery layers in the library context, the focus has remained on its relative interface benefits over the traditional federated search. The informal site- and context specific usability tests have offered little to test the rigor of the discovery layers against the user goals, motivations and workflow they have been designed to support. This study proposes hierarchical task analysis (HTA as an important complementary evaluation method to usability testing of discovery layers. Relevant literature is reviewed for the discovery layers and the HTA method. As no previous application of HTA to the evaluation of discovery layers was found, this paper presents the application of HTA as an expert based and workflow centered (e.g. retrieving a relevant book or a journal article method to evaluating discovery layers. Purdue University’s Primo by Ex Libris was used to map eleven use cases as HTA charts. Nielsen’s Goal Composition theory was used as an analytical framework to evaluate the goal carts from two perspectives: a users’ physical interactions (i.e. clicks, and b user’s cognitive steps (i.e. decision points for what to do next. A brief comparison of HTA and usability test findings is offered as a way of conclusion.

  4. Evaluation of Slow Release Fertilizer Applying Chemical and Spectroscopic methods

    International Nuclear Information System (INIS)

    AbdEl-Kader, A.A.; Al-Ashkar, E.A.

    2005-01-01

    Controlled-release fertilizer offers a number of advantages in relation to crop production in newly reclaimed soils. Butadiene styrene latex emulsion is one of the promising polymer for different purposes. In this work, laboratory evaluation of butadiene styrene latex emulsion 24/76 polymer loaded with a mixed fertilizer was carried out. Macro nutrients (N, P and K) and micro-nutrients(Zn, Fe, and Cu) were extracted by basic extract from the polymer fertilizer mixtures. Micro-sampling technique was investigated and applied to measure Zn, Fe, and Cu using flame atomic absorption spectrometry in order to overcome the nebulization difficulties due to high salt content samples. The cumulative releases of macro and micro-nutrients have been assessed. From the obtained results, it is clear that the release depends on both nutrients and polymer concentration in the mixture. Macro-nutrients are released more efficient than micro-nutrients of total added. Therefore it can be used for minimizing micro-nutrients hazard in soils

  5. Lack of correlation between HRM metrics and symptoms during the manometric protocol.

    Science.gov (United States)

    Xiao, Yinglian; Kahrilas, Peter J; Nicodème, Frédéric; Lin, Zhiyue; Roman, Sabine; Pandolfino, John E

    2014-04-01

    Although esophageal motor disorders are associated with chest pain and dysphagia, minimal data support a direct relationship between abnormal motor function and symptoms. This study investigated whether high-resolution manometry (HRM) metrics correlate with symptoms. Consecutive HRM patients without previous surgery were enrolled. HRM studies included 10 supine liquid, 5 upright liquid, 2 upright viscous, and 2 upright solid swallows. All patients evaluated their esophageal symptom for each upright swallow. Symptoms were graded on a 4-point likert score (0, none; 1, mild; 2, moderate; 3, severe). The individual liquid, viscous or solid upright swallow with the maximal symptom score was selected for analysis in each patient. HRM metrics were compared between groups with and without symptoms during the upright liquid protocol and the provocative protocols separately. A total of 269 patients recorded symptoms during the upright liquid swallows and 72 patients had a swallow symptom score of 1 or greater. Of the 269 patients, 116 recorded symptoms during viscous or solid swallows. HRM metrics were similar between swallows with and without associated symptoms in the upright, viscous, and solid swallows. No correlation was noted between HRM metrics and symptom scores among swallow types. Esophageal symptoms are not related to abnormal motor function defined by HRM during liquid, viscous or solid bolus swallows in the upright position. Other factors beyond circular muscle contraction patterns should be explored as possible causes of symptom generation.

  6. A Comparative Study of Cognitive Schemas of HRM During Economic Crisis in Two Slovenian Sectors

    Directory of Open Access Journals (Sweden)

    Ana Arzenšek

    2012-03-01

    Full Text Available The main objective of this article is to present a qualitative studyof HRM schemas in the recent economic crisis in Slovenia. Cognitiveschemas influence perception and behavior and serve aspowerful sense-making frameworks. In-depth interviews with 16ceos, HR executives and trade unionists from the production andservice sectors and analyses of annual reports were conducted tofind the content of their HRM schemas. In addition, a comparisonbetween schemas from companies from both sectors was made.Results reveal that HRM schemas in companies from the financialsector include more ‘HRM is bureaucratic’ attributes, whereas incompanies from the automotive sector ‘HRM is strategic’ attributesprevail. In companies’ annual reports, the socially responsiblerole of HRM toward employees is only moderately present. Whilethe crisis in 1990s was an influential factor for the sense-makingand ‘flexibility is crucial’ attribution in companies in the automotivesector, the current HRM schema in selected companies fromthe financial sector was influenced by the occurrence of conjuncture.

  7. The lumped heat capacity method applied to target heating

    OpenAIRE

    Rickards, J.

    2013-01-01

    The temperature of metal samples was measured while they were bombarded by the beam from the a particle accelerator. The evolution of the temperature with time can be explained using the lumped heat capacity method of heat transfer. A strong dependence on the type of mounting was found. Se midió la temperatura de muestras metálicas al ser bombardeadas por el haz de iones del Acelerador Pelletron del Instituto de Física. La evolución de la temperatura con el tiempo se puede explicar usando ...

  8. Modern analytic methods applied to the art and archaeology

    International Nuclear Information System (INIS)

    Tenorio C, M. D.; Longoria G, L. C.

    2010-01-01

    The interaction of diverse areas as the analytic chemistry, the history of the art and the archaeology has allowed the development of a variety of techniques used in archaeology, in conservation and restoration. These methods have been used to date objects, to determine the origin of the old materials and to reconstruct their use and to identify the degradation processes that affect the integrity of the art works. The objective of this chapter is to offer a general vision on the researches that have been realized in the Instituto Nacional de Investigaciones Nucleares (ININ) in the field of cultural goods. A series of researches carried out in collaboration with national investigators and of the foreigner is described shortly, as well as with the great support of degree students and master in archaeology of the National School of Anthropology and History, since one of the goals that have is to diffuse the knowledge of the existence of these techniques among the young archaeologists, so that they have a wider vision of what they could use in an in mediate future and they can check hypothesis with scientific methods. (Author)

  9. Benchmark Comparison of Cloud Analytics Methods Applied to Earth Observations

    Science.gov (United States)

    Lynnes, Chris; Little, Mike; Huang, Thomas; Jacob, Joseph; Yang, Phil; Kuo, Kwo-Sen

    2016-01-01

    Cloud computing has the potential to bring high performance computing capabilities to the average science researcher. However, in order to take full advantage of cloud capabilities, the science data used in the analysis must often be reorganized. This typically involves sharding the data across multiple nodes to enable relatively fine-grained parallelism. This can be either via cloud-based file systems or cloud-enabled databases such as Cassandra, Rasdaman or SciDB. Since storing an extra copy of data leads to increased cost and data management complexity, NASA is interested in determining the benefits and costs of various cloud analytics methods for real Earth Observation cases. Accordingly, NASA's Earth Science Technology Office and Earth Science Data and Information Systems project have teamed with cloud analytics practitioners to run a benchmark comparison on cloud analytics methods using the same input data and analysis algorithms. We have particularly looked at analysis algorithms that work over long time series, because these are particularly intractable for many Earth Observation datasets which typically store data with one or just a few time steps per file. This post will present side-by-side cost and performance results for several common Earth observation analysis operations.

  10. Benchmark Comparison of Cloud Analytics Methods Applied to Earth Observations

    Science.gov (United States)

    Lynnes, C.; Little, M. M.; Huang, T.; Jacob, J. C.; Yang, C. P.; Kuo, K. S.

    2016-12-01

    Cloud computing has the potential to bring high performance computing capabilities to the average science researcher. However, in order to take full advantage of cloud capabilities, the science data used in the analysis must often be reorganized. This typically involves sharding the data across multiple nodes to enable relatively fine-grained parallelism. This can be either via cloud-based filesystems or cloud-enabled databases such as Cassandra, Rasdaman or SciDB. Since storing an extra copy of data leads to increased cost and data management complexity, NASA is interested in determining the benefits and costs of various cloud analytics methods for real Earth Observation cases. Accordingly, NASA's Earth Science Technology Office and Earth Science Data and Information Systems project have teamed with cloud analytics practitioners to run a benchmark comparison on cloud analytics methods using the same input data and analysis algorithms. We have particularly looked at analysis algorithms that work over long time series, because these are particularly intractable for many Earth Observation datasets which typically store data with one or just a few time steps per file. This post will present side-by-side cost and performance results for several common Earth observation analysis operations.

  11. Artificial Intelligence Methods Applied to Parameter Detection of Atrial Fibrillation

    Science.gov (United States)

    Arotaritei, D.; Rotariu, C.

    2015-09-01

    In this paper we present a novel method to develop an atrial fibrillation (AF) based on statistical descriptors and hybrid neuro-fuzzy and crisp system. The inference of system produce rules of type if-then-else that care extracted to construct a binary decision system: normal of atrial fibrillation. We use TPR (Turning Point Ratio), SE (Shannon Entropy) and RMSSD (Root Mean Square of Successive Differences) along with a new descriptor, Teager- Kaiser energy, in order to improve the accuracy of detection. The descriptors are calculated over a sliding window that produce very large number of vectors (massive dataset) used by classifier. The length of window is a crisp descriptor meanwhile the rest of descriptors are interval-valued type. The parameters of hybrid system are adapted using Genetic Algorithm (GA) algorithm with fitness single objective target: highest values for sensibility and sensitivity. The rules are extracted and they are part of the decision system. The proposed method was tested using the Physionet MIT-BIH Atrial Fibrillation Database and the experimental results revealed a good accuracy of AF detection in terms of sensitivity and specificity (above 90%).

  12. Frequency domain methods applied to forecasting electricity markets

    International Nuclear Information System (INIS)

    Trapero, Juan R.; Pedregal, Diego J.

    2009-01-01

    The changes taking place in electricity markets during the last two decades have produced an increased interest in the problem of forecasting, either load demand or prices. Many forecasting methodologies are available in the literature nowadays with mixed conclusions about which method is most convenient. This paper focuses on the modeling of electricity market time series sampled hourly in order to produce short-term (1 to 24 h ahead) forecasts. The main features of the system are that (1) models are of an Unobserved Component class that allow for signal extraction of trend, diurnal, weekly and irregular components; (2) its application is automatic, in the sense that there is no need for human intervention via any sort of identification stage; (3) the models are estimated in the frequency domain; and (4) the robustness of the method makes possible its direct use on both load demand and price time series. The approach is thoroughly tested on the PJM interconnection market and the results improve on classical ARIMA models. (author)

  13. Interesting Developments in Testing Methods Applied to Foundation Piles

    Science.gov (United States)

    Sobala, Dariusz; Tkaczyński, Grzegorz

    2017-10-01

    Both: piling technologies and pile testing methods are a subject of current development. New technologies, providing larger diameters or using in-situ materials, are very demanding in terms of providing proper quality of execution of works. That concerns the material quality and continuity which define the integral strength of pile. On the other side we have the capacity of the ground around the pile and its ability to carry the loads transferred by shaft and pile base. Inhomogeneous nature of soils and a relatively small amount of tested piles imposes very good understanding of small amount of results. In some special cases the capacity test itself form an important cost in the piling contract. This work presents a brief description of selected testing methods and authors remarks based on cooperation with Universities constantly developing new ideas. Paper presents some experience based remarks on integrity testing by means of low energy impact (low strain) and introduces selected (Polish) developments in the field of closed-end pipe piles testing based on bi-directional loading, similar to Osterberg idea, but without sacrificial hydraulic jack. Such test is suitable especially when steel piles are used for temporary support in the rivers, where constructing of conventional testing appliance with anchor piles or kentledge meets technical problems. According to the author’s experience, such tests were not yet used on the building site but they bring a real potential especially, when the displacement control can be provided from the river bank using surveying techniques.

  14. Applying Simulation Method in Formulation of Gluten-Free Cookies

    Directory of Open Access Journals (Sweden)

    Nikitina Marina

    2017-01-01

    Full Text Available At present time priority direction in the development of new food products its developing of technology products for special purposes. These types of products are gluten-free confectionery products, intended for people with celiac disease. Gluten-free products are in demand among consumers, it needs to expand assortment, and improvement of quality indicators. At this article results of studies on the development of pastry products based on amaranth flour does not contain gluten. Study based on method of simulation recipes gluten-free confectionery functional orientation to optimize their chemical composition. The resulting products will allow to diversify and supplement the necessary nutrients diet for people with gluten intolerance, as well as for those who follow a gluten-free diet.

  15. Nuclear method applied in archaeological sites at the Amazon basin

    International Nuclear Information System (INIS)

    Nicoli, Ieda Gomes; Bernedo, Alfredo Victor Bellido; Latini, Rose Mary

    2002-01-01

    The aim of this work was to use the nuclear methodology to character pottery discovered inside archaeological sites recognized with circular earth structure in Acre State - Brazil which may contribute to the research in the reconstruction of part of the pre-history of the Amazonic Basin. The sites are located mainly in the Hydrographic Basin of High Purus River. Three of them were strategic chosen to collect the ceramics: Lobao, in Sena Madureira County at north; Alto Alegre in Rio Branco County at east and Xipamanu I, in Xapuri County at south. Neutron Activation Analysis in conjunction with multivariate statistical methods were used for the ceramic characterization and classification. An homogeneous group was established by all the sherds collected from Alto Alegre and was distinct from the other two groups analyzed. Some of the sherds collected from Xipamunu I appeared in Lobao's urns, probably because they had the same fabrication process. (author)

  16. Applying Multi-Criteria Analysis Methods for Fire Risk Assessment

    Directory of Open Access Journals (Sweden)

    Pushkina Julia

    2015-11-01

    Full Text Available The aim of this paper is to prove the application of multi-criteria analysis methods for optimisation of fire risk identification and assessment process. The object of this research is fire risk and risk assessment. The subject of the research is studying the application of analytic hierarchy process for modelling and influence assessment of various fire risk factors. Results of research conducted by the authors can be used by insurance companies to perform the detailed assessment of fire risks on the object and to calculate a risk extra charge to an insurance premium; by the state supervisory institutions to determine the compliance of a condition of object with requirements of regulations; by real state owners and investors to carry out actions for decrease in degree of fire risks and minimisation of possible losses.

  17. Applied statistical methods in agriculture, health and life sciences

    CERN Document Server

    Lawal, Bayo

    2014-01-01

    This textbook teaches crucial statistical methods to answer research questions using a unique range of statistical software programs, including MINITAB and R. This textbook is developed for undergraduate students in agriculture, nursing, biology and biomedical research. Graduate students will also find it to be a useful way to refresh their statistics skills and to reference software options. The unique combination of examples is approached using MINITAB and R for their individual strengths. Subjects covered include among others data description, probability distributions, experimental design, regression analysis, randomized design and biological assay. Unlike other biostatistics textbooks, this text also includes outliers, influential observations in regression and an introduction to survival analysis. Material is taken from the author's extensive teaching and research in Africa, USA and the UK. Sample problems, references and electronic supplementary material accompany each chapter.

  18. A new deconvolution method applied to ultrasonic images

    International Nuclear Information System (INIS)

    Sallard, J.

    1999-01-01

    This dissertation presents the development of a new method for restoration of ultrasonic signals. Our goal is to remove the perturbations induced by the ultrasonic probe and to help to characterize the defects due to a strong local discontinuity of the acoustic impedance. The point of view adopted consists in taking into account the physical properties in the signal processing to develop an algorithm which gives good results even on experimental data. The received ultrasonic signal is modeled as a convolution between a function that represents the waveform emitted by the transducer and a function that is abusively called the 'defect impulse response'. It is established that, in numerous cases, the ultrasonic signal can be expressed as a sum of weighted, phase-shifted replicas of a reference signal. Deconvolution is an ill-posed problem. A priori information must be taken into account to solve the problem. The a priori information translates the physical properties of the ultrasonic signals. The defect impulse response is modeled as a Double-Bernoulli-Gaussian sequence. Deconvolution becomes the problem of detection of the optimal Bernoulli sequence and estimation of the associated complex amplitudes. Optimal parameters of the sequence are those which maximize a likelihood function. We develop a new estimation procedure based on an optimization process. An adapted initialization procedure and an iterative algorithm enables to quickly process a huge number of data. Many experimental ultrasonic data that reflect usual control configurations have been processed and the results demonstrate the robustness of the method. Our algorithm enables not only to remove the waveform emitted by the transducer but also to estimate the phase. This parameter is useful for defect characterization. At last the algorithm makes easier data interpretation by concentrating information. So automatic characterization should be possible in the future. (author)

  19. Applying Human-Centered Design Methods to Scientific Communication Products

    Science.gov (United States)

    Burkett, E. R.; Jayanty, N. K.; DeGroot, R. M.

    2016-12-01

    Knowing your users is a critical part of developing anything to be used or experienced by a human being. User interviews, journey maps, and personas are all techniques commonly employed in human-centered design practices because they have proven effective for informing the design of products and services that meet the needs of users. Many non-designers are unaware of the usefulness of personas and journey maps. Scientists who are interested in developing more effective products and communication can adopt and employ user-centered design approaches to better reach intended audiences. Journey mapping is a qualitative data-collection method that captures the story of a user's experience over time as related to the situation or product that requires development or improvement. Journey maps help define user expectations, where they are coming from, what they want to achieve, what questions they have, their challenges, and the gaps and opportunities that can be addressed by designing for them. A persona is a tool used to describe the goals and behavioral patterns of a subset of potential users or customers. The persona is a qualitative data model that takes the form of a character profile, built upon data about the behaviors and needs of multiple users. Gathering data directly from users avoids the risk of basing models on assumptions, which are often limited by misconceptions or gaps in understanding. Journey maps and user interviews together provide the data necessary to build the composite character that is the persona. Because a persona models the behaviors and needs of the target audience, it can then be used to make informed product design decisions. We share the methods and advantages of developing and using personas and journey maps to create more effective science communication products.

  20. Når HRM sparker døren ind til fremtiden

    DEFF Research Database (Denmark)

    Holt Larsen, Henrik

    2016-01-01

    Vi er alt for beskedne of uambitiøse, når vi siger, hvad HRM kan bidrage med i fremtidens virksomheder. Mange af de generelle udfordringer, som virksomhederne står overfor, har nemlig en karakter, der gør det naturligt eller nødvendigt at sætte ind med HRM-professionalisme. Bolden ligger for vores...... fødder, så nu gælder det bare om at sparke. Artiklen beskriver nogle af de vigtigste udfordringer - og hvorfor HRM er et svar herpå....

  1. CI in the work place: does involving the HRM function make any difference?

    DEFF Research Database (Denmark)

    Hyland, Paul; Becker, Karen; Sload, Terry

    2008-01-01

    benefit the organisation's performance. The HRM function is often given the task of championing cultural change and managing aspects of training and learning, and it would appear that involvement of HRM professionals would enhance CI efforts and assist in the timely solution of issues within the CI......People are central to successful Continuous Improvement (CI), and in larger organisations a Human Resource Management (HRM) function is responsible for people related issues. Central to CI is learning and a culture that supports CI. Learning needs to be both individual and organisational, and must...

  2. Applying the partitioned multiobjective risk method (PMRM) to portfolio selection.

    Science.gov (United States)

    Reyes Santos, Joost; Haimes, Yacov Y

    2004-06-01

    The analysis of risk-return tradeoffs and their practical applications to portfolio analysis paved the way for Modern Portfolio Theory (MPT), which won Harry Markowitz a 1992 Nobel Prize in Economics. A typical approach in measuring a portfolio's expected return is based on the historical returns of the assets included in a portfolio. On the other hand, portfolio risk is usually measured using volatility, which is derived from the historical variance-covariance relationships among the portfolio assets. This article focuses on assessing portfolio risk, with emphasis on extreme risks. To date, volatility is a major measure of risk owing to its simplicity and validity for relatively small asset price fluctuations. Volatility is a justified measure for stable market performance, but it is weak in addressing portfolio risk under aberrant market fluctuations. Extreme market crashes such as that on October 19, 1987 ("Black Monday") and catastrophic events such as the terrorist attack of September 11, 2001 that led to a four-day suspension of trading on the New York Stock Exchange (NYSE) are a few examples where measuring risk via volatility can lead to inaccurate predictions. Thus, there is a need for a more robust metric of risk. By invoking the principles of the extreme-risk-analysis method through the partitioned multiobjective risk method (PMRM), this article contributes to the modeling of extreme risks in portfolio performance. A measure of an extreme portfolio risk, denoted by f(4), is defined as the conditional expectation for a lower-tail region of the distribution of the possible portfolio returns. This article presents a multiobjective problem formulation consisting of optimizing expected return and f(4), whose solution is determined using Evolver-a software that implements a genetic algorithm. Under business-as-usual market scenarios, the results of the proposed PMRM portfolio selection model are found to be compatible with those of the volatility-based model

  3. Simplified Methods Applied to Nonlinear Motion of Spar Platforms

    Energy Technology Data Exchange (ETDEWEB)

    Haslum, Herbjoern Alf

    2000-07-01

    Simplified methods for prediction of motion response of spar platforms are presented. The methods are based on first and second order potential theory. Nonlinear drag loads and the effect of the pumping motion in a moon-pool are also considered. Large amplitude pitch motions coupled to extreme amplitude heave motions may arise when spar platforms are exposed to long period swell. The phenomenon is investigated theoretically and explained as a Mathieu instability. It is caused by nonlinear coupling effects between heave, surge, and pitch. It is shown that for a critical wave period, the envelope of the heave motion makes the pitch motion unstable. For the same wave period, a higher order pitch/heave coupling excites resonant heave response. This mutual interaction largely amplifies both the pitch and the heave response. As a result, the pitch/heave instability revealed in this work is more critical than the previously well known Mathieu's instability in pitch which occurs if the wave period (or the natural heave period) is half the natural pitch period. The Mathieu instability is demonstrated both by numerical simulations with a newly developed calculation tool and in model experiments. In order to learn more about the conditions for this instability to occur and also how it may be controlled, different damping configurations (heave damping disks and pitch/surge damping fins) are evaluated both in model experiments and by numerical simulations. With increased drag damping, larger wave amplitudes and more time are needed to trigger the instability. The pitch/heave instability is a low probability of occurrence phenomenon. Extreme wave periods are needed for the instability to be triggered, about 20 seconds for a typical 200m draft spar. However, it may be important to consider the phenomenon in design since the pitch/heave instability is very critical. It is also seen that when classical spar platforms (constant cylindrical cross section and about 200m draft

  4. Variational methods applied to problems of diffusion and reaction

    CERN Document Server

    Strieder, William

    1973-01-01

    This monograph is an account of some problems involving diffusion or diffusion with simultaneous reaction that can be illuminated by the use of variational principles. It was written during a period that included sabbatical leaves of one of us (W. S. ) at the University of Minnesota and the other (R. A. ) at the University of Cambridge and we are grateful to the Petroleum Research Fund for helping to support the former and the Guggenheim Foundation for making possible the latter. We would also like to thank Stephen Prager for getting us together in the first place and for showing how interesting and useful these methods can be. We have also benefitted from correspondence with Dr. A. M. Arthurs of the University of York and from the counsel of Dr. B. D. Coleman the general editor of this series. Table of Contents Chapter 1. Introduction and Preliminaries . 1. 1. General Survey 1 1. 2. Phenomenological Descriptions of Diffusion and Reaction 2 1. 3. Correlation Functions for Random Suspensions 4 1. 4. Mean Free ...

  5. Nondestructive methods of analysis applied to oriental swords

    Directory of Open Access Journals (Sweden)

    Edge, David

    2015-12-01

    Full Text Available Various neutron techniques were employed at the Budapest Nuclear Centre in an attempt to find the most useful method for analysing the high-carbon steels found in Oriental arms and armour, such as those in the Wallace Collection, London. Neutron diffraction was found to be the most useful in terms of identifying such steels and also indicating the presence of hidden patternEn el Centro Nuclear de Budapest se han empleado varias técnicas neutrónicas con el fin de encontrar un método adecuado para analizar las armas y armaduras orientales con un alto contenido en carbono, como algunas de las que se encuentran en la Colección Wallace de Londres. El empleo de la difracción de neutrones resultó ser la técnica más útil de cara a identificar ese tipo de aceros y también para encontrar patrones escondidos.

  6. Perturbation Method of Analysis Applied to Substitution Measurements of Buckling

    Energy Technology Data Exchange (ETDEWEB)

    Persson, Rolf

    1966-11-15

    Calculations with two-group perturbation theory on substitution experiments with homogenized regions show that a condensation of the results into a one-group formula is possible, provided that a transition region is introduced in a proper way. In heterogeneous cores the transition region comes in as a consequence of a new cell concept. By making use of progressive substitutions the properties of the transition region can be regarded as fitting parameters in the evaluation procedure. The thickness of the region is approximately equal to the sum of 1/(1/{tau} + 1/L{sup 2}){sup 1/2} for the test and reference regions. Consequently a region where L{sup 2} >> {tau}, e.g. D{sub 2}O, contributes with {radical}{tau} to the thickness. In cores where {tau} >> L{sup 2} , e.g. H{sub 2}O assemblies, the thickness of the transition region is determined by L. Experiments on rod lattices in D{sub 2}O and on test regions of D{sub 2}O alone (where B{sup 2} = - 1/L{sup 2} ) are analysed. The lattice measurements, where the pitches differed by a factor of {radical}2, gave excellent results, whereas the determination of the diffusion length in D{sub 2}O by this method was not quite successful. Even regions containing only one test element can be used in a meaningful way in the analysis.

  7. Complexity methods applied to turbulence in plasma astrophysics

    Science.gov (United States)

    Vlahos, L.; Isliker, H.

    2016-09-01

    In this review many of the well known tools for the analysis of Complex systems are used in order to study the global coupling of the turbulent convection zone with the solar atmosphere where the magnetic energy is dissipated explosively. Several well documented observations are not easy to interpret with the use of Magnetohydrodynamic (MHD) and/or Kinetic numerical codes. Such observations are: (1) The size distribution of the Active Regions (AR) on the solar surface, (2) The fractal and multi fractal characteristics of the observed magnetograms, (3) The Self-Organised characteristics of the explosive magnetic energy release and (4) the very efficient acceleration of particles during the flaring periods in the solar corona. We review briefly the work published the last twenty five years on the above issues and propose solutions by using methods borrowed from the analysis of complex systems. The scenario which emerged is as follows: (a) The fully developed turbulence in the convection zone generates and transports magnetic flux tubes to the solar surface. Using probabilistic percolation models we were able to reproduce the size distribution and the fractal properties of the emerged and randomly moving magnetic flux tubes. (b) Using a Non Linear Force Free (NLFF) magnetic extrapolation numerical code we can explore how the emerged magnetic flux tubes interact nonlinearly and form thin and Unstable Current Sheets (UCS) inside the coronal part of the AR. (c) The fragmentation of the UCS and the redistribution of the magnetic field locally, when the local current exceeds a Critical threshold, is a key process which drives avalanches and forms coherent structures. This local reorganization of the magnetic field enhances the energy dissipation and influences the global evolution of the complex magnetic topology. Using a Cellular Automaton and following the simple rules of Self Organized Criticality (SOC), we were able to reproduce the statistical characteristics of the

  8. The Research in e-HRM: Relevance and Implications in the Knowledge Society

    Directory of Open Access Journals (Sweden)

    Sofia TOTOLICI

    2012-11-01

    Full Text Available This article aims to review current empirical research on electronic Human Resource Management (e-HRM and discusses some implications for future research, which will be focused on comparative analysis of how social and cultural factors might influence the implementation and development of e-HRM systems in different E.U. countries. Based on a definition and an initial framework, we analyzed the surveys conducted in this field as well as the case studies focused on practical e-HRM applications, the examined topics and the relevant findings. Another goal of this study is to highlight the gaps between e-HRM and HRIS (Human resource information system, which refers to ICT systems used within HR departments We are also interested in assessing the opportunities provided by Web 2.0 technologies for e-recruitment, the first field of human resource management to make extensive use of web-based technology.

  9. Understanding the Indigenous Chinese Concept of Suzhi (素质) from an HRM Perspective

    DEFF Research Database (Denmark)

    Wang, Greg; Lamond, David; Worm, Verner

    2014-01-01

    and multidimensional frameworks, suzhi criteria may form different gestalts in different organizations and industries. Research limitations/implications - From a sociocultural and historical perspective, HRM research that incorporates a combination of indigenous and indigenized suzhi characteristics may receive better...

  10. Rapid and inexpensive body fluid identification by RNA profiling-based multiplex High Resolution Melt (HRM analysis [v1; ref status: indexed, http://f1000r.es/2hj

    Directory of Open Access Journals (Sweden)

    Erin K. Hanson

    2013-12-01

    Full Text Available Positive identification of the nature of biological material present on evidentiary items can be crucial for understanding the circumstances surrounding a crime. However, traditional protein-based methods do not permit the identification of all body fluids and tissues, and thus molecular based strategies for the conclusive identification of all forensically relevant biological fluids and tissues need to be developed. Messenger RNA (mRNA profiling is an example of such a molecular-based approach. Current mRNA body fluid identification assays involve capillary electrophoresis (CE or quantitative RT-PCR (qRT-PCR platforms, each with its own limitations. Both platforms require the use of expensive fluorescently labeled primers or probes. CE-based assays require separate amplification and detection steps thus increasing the analysis time. For qRT-PCR assays, only 3-4 markers can be included in a single reaction since each requires a different fluorescent dye. To simplify mRNA profiling assays, and reduce the time and cost of analysis, we have developed single- and multiplex body fluid High Resolution Melt (HRM assays for the identification of common forensically relevant biological fluids and tissues. The incorporated biomarkers include IL19 (vaginal secretions, IL1F7 (skin, ALAS2 (blood, MMP10 (menstrual blood, HTN3 (saliva and TGM4 (semen.  The HRM assays require only unlabeled PCR primers and a single saturating intercalating fluorescent dye (Eva Green. Each body-fluid-specific marker can easily be identified by the presence of a distinct melt peak. Usually, HRM assays are used to detect variants or isoforms for a single gene target. However, we have uniquely developed duplex and triplex HRM assays to permit the simultaneous detection of multiple targets per reaction. Here we describe the development and initial performance evaluation of the developed HRM assays. The results demonstrate the potential use of HRM assays for rapid, and relatively

  11. Near-infrared radiation curable multilayer coating systems and methods for applying same

    Science.gov (United States)

    Bowman, Mark P; Verdun, Shelley D; Post, Gordon L

    2015-04-28

    Multilayer coating systems, methods of applying and related substrates are disclosed. The coating system may comprise a first coating comprising a near-IR absorber, and a second coating deposited on a least a portion of the first coating. Methods of applying a multilayer coating composition to a substrate may comprise applying a first coating comprising a near-IR absorber, applying a second coating over at least a portion of the first coating and curing the coating with near infrared radiation.

  12. The role of HRM practices in building intellectual capital in knowledge-based teams

    DEFF Research Database (Denmark)

    Jørgensen, Frances; Becker, Karen

    2013-01-01

    In this paper, we investigate the ways HRM systems support development of intellectual capital in teams in three case studies of knowledge-intensive firms (KIFs) in Denmark. By focusing on team composition and structure, findings build on the rapidly growing body of literature on HRM’s role....... The findings also have managerial implications regarding the need to align team composition and structure, HRM systems, and intellectual capital focus to support development of team-based intellectual capital....

  13. Work-Life Balance Practices Among Irish Hotel Employees:Implications for HRM

    OpenAIRE

    Farrell, Kathleen

    2015-01-01

    The aim of this paper is to examine work-life balance in the Irish hotel sector from an employee perspective with implications for HRM. 246 questionnaires from employees were returned which was a 22% response rate. Company benefits were not associated with numerical flexibility, but company benefits were associated with functional flexibility and work-life balance supports. This would suggest an integrated approach to human resource management (HRM), whereby some companies engage in a contemp...

  14. Development of a Causal Framework linking High Perofrmance HRM Practices, Positive Psychological Capital, Creative Behaviours

    OpenAIRE

    Gupta, Vishal

    2013-01-01

    Synthesizing the ideas of high-performance Human Resource Management (HRM), positive psychological capital, and componential theory of creativity, the present study develops a multi-level causal framework linking high-performance work practices (HPWP), positive psychological capital, employee creative performance behaviors and creative performance. The paper argues that to provide a convincing explanation of the association between HRM practices and creativity, we need to improve our theoreti...

  15. Detection of plant oil DNA using high resolution melting (HRM) post PCR analysis: a tool for disclosure of olive oil adulteration.

    Science.gov (United States)

    Vietina, Michelangelo; Agrimonti, Caterina; Marmiroli, Nelson

    2013-12-15

    Extra virgin olive oil is frequently subjected to adulterations with addition of oils obtained from plants other than olive. DNA analysis is a fast and economic tool to identify plant components in oils. Extraction and amplification of DNA by PCR was tested in olives, in milled seeds and in oils, to investigate its use in olive oil traceability. DNA was extracted from different oils made of hazelnut, maize, sunflower, peanut, sesame, soybean, rice and pumpkin. Comparing the DNA melting profiles in reference plant materials and in the oils, it was possible to identify any plant components in oils and mixtures of oils. Real-Time PCR (RT-PCR) platform has been added of the new methodology of high resolution melting (HRM), both were used to analyse olive oils mixed with different percentage of other oils. Results showed HRM a cost effective method for efficient detection of adulterations in olive oils. Copyright © 2013 Elsevier Ltd. All rights reserved.

  16. Further Insight and Additional Inference Methods for Polynomial Regression Applied to the Analysis of Congruence

    Science.gov (United States)

    Cohen, Ayala; Nahum-Shani, Inbal; Doveh, Etti

    2010-01-01

    In their seminal paper, Edwards and Parry (1993) presented the polynomial regression as a better alternative to applying difference score in the study of congruence. Although this method is increasingly applied in congruence research, its complexity relative to other methods for assessing congruence (e.g., difference score methods) was one of the…

  17. Using qualitative repertory grid interviews to gather shared perspectives in a sequential mixed methods research design

    OpenAIRE

    Rojon, C; Saunders, M.N.K.; McDowall, Almuth

    2016-01-01

    In this chapter, we consider a specific example of applying mixed methods designs combining both qualitative and quantitative data collection and analysis approaches, giving particular attention to issues including reliability and validity. Human resource management (HRM) researchers, like others setting out to examine a novel or insufficiently defined research topic, frequently favour qualitative approaches to gather data during initial stages, to facilitate an in-depth exploration of indivi...

  18. Identification and Differentiation of Monilinia Species Causing Brown Rot of Pome and Stone Fruit using High-Resolution Melting (HRM) Analysis.

    Science.gov (United States)

    Papavasileiou, Antonios; Madesis, Panagiotis B; Karaoglanidis, George S

    2016-09-01

    Brown rot is a devastating disease of stone fruit caused by Monilinia spp. Among these species, Monilinia fructicola is a quarantine pathogen in Europe but has recently been detected in several European countries. Identification of brown rot agents relies on morphological differences or use of molecular methods requiring fungal isolation. The current study was initiated to develop and validate a high-resolution melting (HRM) method for the identification of the Monilinia spp. and for the detection of M. fructicola among other brown rot pathogens. Based on the sequence of the cytb intron from M. laxa, M. fructicola, M. fructigena, M. mumecola, M. linhartiana, and M. yunnanensis isolates originating from several countries, a pair of universal primers for species identification and a pair of primers specific to M. fructicola were designed. The specificity of the primers was verified to ensure against cross-reaction with other fungal species. The melting curve analysis using the universal primers generated six different HRM curve profiles, each one specific for each species. Τhe HRM analysis primers specific to M. fructicola amplified a 120-bp region with a distinct melt profile corresponding to the presence of M. fructicola, regardless of the presence of other species. HRM analysis can be a useful tool for rapid identification and differentiation of the six Monilinia spp. using a single primer pair. This novel assay has the potential for simultaneous identification and differentiation of the closely related Monilinia spp. as well as for the differentiation of M. fructicola from other common pathogens or saprophytes that may occur on the diseased fruit.

  19. Mutation spectrum of 122 hemophilia A families from Taiwanese population by LD-PCR, DHPLC, multiplex PCR and evaluating the clinical application of HRM

    Directory of Open Access Journals (Sweden)

    Chang Chieh-Ting

    2008-06-01

    Full Text Available Abstract Background Hemophilia A represents the most common and severe inherited hemorrhagic disorder. It is caused by mutations in the F8 gene, which leads to a deficiency or dysfunctional factor VIII protein, an essential cofactor in the factor X activation complex. Methods We used long-distance polymerase chain reaction and denaturing high performance liquid chromatography for mutation scanning of the F8 gene. We designed the competitive multiplex PCR to identify the carrier with exonal deletions. In order to facilitate throughput and minimize the cost of mutation scanning, we also evaluated a new mutation scanning technique, high resolution melting analysis (HRM, as an alternative screening method. Results We presented the results of detailed screening of 122 Taiwanese families with hemophilia A and reported twenty-nine novel mutations. There was one family identified with whole exons deletion, and the carriers were successfully recognized by multiplex PCR. By HRM, the different melting curve patterns were easily identified in 25 out of 28 cases (89% and 15 out of 15 (100% carriers. The sensitivity was 93 % (40/43. The overall mutation detection rate of hemophilia A was 100% in this study. Conclusion We proposed a diagnostic strategy for hemophilia A genetic diagnosis. We consider HRM as a powerful screening tool that would provide us with a more cost-effective protocol for hemophilia A mutation identification.

  20. [Application of three heat pulse technique-based methods to determine the stem sap flow].

    Science.gov (United States)

    Wang, Sheng; Fan, Jun

    2015-08-01

    It is of critical importance to acquire tree transpiration characters through sap flow methodology to understand tree water physiology, forest ecology and ecosystem water exchange. Tri-probe heat pulse sensors, which are widely utilized in soil thermal parameters and soil evaporation measurement, were applied to implement Salix matsudana sap flow density (Vs) measurements via heat-ratio method (HRM), T-Max method (T-Max) and single-probe heat pulse probe (SHPP) method, and comparative analysis was conducted with additional Grainer's thermal diffusion probes (TDP) measured results. The results showed that, it took about five weeks to reach a stable measurement stage after TPHP installation, Vs measured with three methods in the early stage after installation was 135%-220% higher than Vs in the stable measurement stage, and Vs estimated via HRM, T-Max and SHPP methods were significantly linearly correlated with Vs estimated via TDP method, with R2 of 0.93, 0.73 and 0.91, respectively, and R2 for Vs measured by SHPP and HRM reached 0.94. HRM had relatively higher precision in measuring low rates and reverse sap flow. SHPP method seemed to be very promising to measure sap flow for configuration simplicity and high measuring accuracy, whereas it couldn' t distinguish directions of flow. T-Max method had relatively higher error in sap flow measurement, and it couldn' t measure sap flow below 5 cm3 · cm(-2) · h(-1), thus this method could not be used alone, however it could measure thermal diffusivity for calculating sap flow when other methods were imposed. It was recommended to choose a proper method or a combination of several methods to measure stem sap flow, based on specific research purpose.

  1. High resolution melting (HRM) analysis as a new tool for rapid identification of Salmonella enterica serovar Gallinarum biovars Pullorum and Gallinarum.

    Science.gov (United States)

    Ren, Xingxing; Fu, Ying; Xu, Chenggang; Feng, Zhou; Li, Miao; Zhang, Lina; Zhang, Jianmin; Liao, Ming

    2017-05-01

    Salmonella enterica serovar Gallinarum biovars Pullorum and Gallinarum represent the most common causative agents of chicken salmonellosis, which result in high mortality and morbidity throughout the world. It is difficult and laborious to discriminate these diseases based on biochemical or phenotypic methods. Herein, we report the development of a single nucleotide polymorphism (SNP) PCR-high resolution melt (PCR-HRM) assay for the detection and discrimination of both S. Pullorum and S. Gallinarun. The gene rfbS, which encodes a factor involved in the biosynthesis of ADP paratose in serogroup D of Salmonella, has been identified as a robust genetic marker for the identification of S. Pullorum and S. Gallinarun based on polymorphisms at positions 237 and 598. Therefore, PCR-HRM analyses were used to characterize this gene. A total of 15 reference and 33 clinical isolates of Salmonella and related Gram-negative bacteria were detected using 2 sets of primers. Our PCR-HRM assay could distinguish S. Pullorum from S. Gallinarun and other strains using the primer pair SP-237F/237R. Similarly, S. Gallinarun could be distinguished from S. Pullorum and other strains using primer set SG-598F/598R. These 2 assays showed high specificity (100%) for both S. Pullorum and S. Gallinarun; the sensitivity of these 2 assays was at least 100-fold greater than that of the allele-specific PCR assay. This present study demonstrated that HRM analysis represents a potent, simple, and economic tool for the rapid, specific, and sensitive detection of S. Pullorum and S. Gallinarun. Our approach also may aid efforts for purification of Avian Salmonella disease. © 2016 Poultry Science Association Inc.

  2. Linking HRM and small business performance : An examination of the impact of HRM intensity on the productivity and financial performance of small businesses

    NARCIS (Netherlands)

    Sels, L; De Winne, S; Delmotte, J; Maes, J; Faems, D; Forrier, A

    Attempts to explore empirically the link between HRM and firm performance are numerous. Yet, research on this link remains restricted to large companies. Little is known about the extent to which the existing results extend to small businesses. The purpose of the present study is to develop and test

  3. Genus-Specific Real-Time PCR and HRM Assays to Distinguish Liriope from Ophiopogon Samples.

    Science.gov (United States)

    Masiero, Eva; Banik, Dipanwita; Abson, John; Greene, Paul; Slater, Adrian; Sgamma, Tiziana

    2017-10-26

    Liriope and Ophiopogon species have a long history of use as traditional medicines across East Asia. They have also become widely used around the world for ornamental and landscaping purposes. The morphological similarities between Liriope and Ophiopogon taxa have made the taxonomy of the two genera problematic and caused confusion about the identification of individual specimens. Molecular approaches could be a useful tool for the discrimination of these two genera in combination with traditional methods. Seventy-five Liriope and Ophiopogon samples from the UK National Plant Collections of Ophiopogon and Liriope were analyzed. The 5' end of the DNA barcode region of the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase ( rbcLa ) was used for the discrimination of the two genera. A single nucleotide polymorphism (SNP) between the two genera allowed the development of discriminatory tests for genus-level identification based on specific PCR and high-resolution melt curve (HRM) assays. The study highlights the advantage of incorporating DNA barcoding methods into plant identification protocols and provides simple assays that could be used for the quality assurance of commercially traded plants and herbal drugs.

  4. Successful Models of Implementation of e-HRM Systems in the Private Sector in Romania

    Directory of Open Access Journals (Sweden)

    Aurel TOTOLICI

    2013-12-01

    Full Text Available The essential condition for attaining excellence in the informatized administration of the Human Resources Management (HRM activities is the development and implementation of high-performance e-HRM systems. For this reason, any organization, regardless of her nature and dimensions, needs to make use of the implementation of an e-HRM IT system adapted to its specific needs, because the performances of the HR activities largely depend on the correlation of the software tools with the employees’ abilities. The identification of the benefits of the e-HRM systems and the impact of these systems on the managerial and, implicitly, organizational performances have become highly interesting subjects, both among the theorists and among the practitioners worldwide. In order to detail the effects and the benefits of the implementation of the e-HRM system, we considered it useful to present a case study focused on a private firm of Romania: Arcelor Mittal Company of Galaţi, namely a Steel Mill.

  5. The impact of demographical variables on HRM practices in Saudi Telecom Company: An empirical exploration

    Directory of Open Access Journals (Sweden)

    Nasser S. Al-Kahtani

    2018-03-01

    Full Text Available Fair HRM practices are indispensable for the success of any company, especially the growing companies. The aim of this exploration is to understand the impact of certain personal characteris-tics of the employees of Telecommunication Company on HRM practices in the Kingdom of Saudi Arabia. A booklet consisted of HRM practices questionnaires along with personal characteristics blank sheet were used to gather the information of the respondents. A total of 210 sample collected from public and private sector Telecommunication Company existing in the Kingdom. The collected data was analyzed by inferential and descriptive statistics and the results were analyzed. The results revealed that married & unmarried, public & private sector, younger & older, high & low income and high & low experienced group of employees differ but in a varied degree on HRM practices in terms of performance appraisal, training and development, security and health, recruitment and se-lection, intention to stay in the company communication, working conditions, compensation and benefits, job analysis and co-worker relationships. The findings’ significances and implications were also elaborated at length and few suggestions were provided that could enhance the fair HRM practices in the telecommunication company in the Kingdom.

  6. Buffers or Boosters? The Role of HRM Practices in Older Workers' Experience of Stereotype Threat.

    Science.gov (United States)

    Oliveira, Eduardo André da Silva; Cabral-Cardoso, Carlos José

    2018-01-02

    Building on the social identity approach and the HRM literature, this two-wave cross-sectional study examined the effects of negative age-based metastereotypes on the age-based stereotype threat experience of older workers and on organizational disidentification. The moderator role of HRM practices in the relationship between negative age-based metastereotypes and age-based stereotype threat was also investigated. Older workers survey results (n = 469) from 14 manufacturing companies indicated that negative age-based metastereotypes correlate positively with stereotype threat. Moderation results showed that age-awareness HRM practices (training for older workers) reinforced age-based stereotype threat, whereas general HRM practices (recognition and respect) impaired it. The article suggests that the combination of age-based metastereotypes with a stereotype threat framework contributes to further understand older workers' beliefs and attitudes. It also indicates that to be effective, HRM practices should emphasize positive social identities older workers share with their colleagues, rather than giving older workers special treatment that may, after all, reinforce stigmatization.

  7. MEDIATING ROLE OF EMPLOYEE MOTIVATION IN RELATIONSHIP TO POST-SELECTION HRM PRACTICES AND ORGANIZATIONAL PERFORMANCE

    Directory of Open Access Journals (Sweden)

    Aftab Tariq Dar

    2014-07-01

    Full Text Available The purpose of this cross-sectional study was to explore the meditating role of employee motivation in the relationship between post-selection Human Resource Management (HRM practices and perceived performance of Islamic Banks operating in Pakistan. This study was based on primary data collected from 200-employees of different Islamic banks in Pakistan by distributing structured and reliable questionnaires. The demographic profile of respondents was diversified in terms of their grade, age, experience and education. Results of correlation analysis revealed that training & development(r = .829, performance evaluation system(r = .752, career development system(r = .666, extrinsic rewards(r = .921, intrinsic rewards (r = .852 and employee motivation(r =.722 have strong positive association with perceived performance of Islamic banks in Pakistan. Results of hierarchical regression analysis supported that post-selection HRM practices have positive impact on perceived organizational performance and employee motivation acts as mediator in the relationship between post-selection HRM practices and perceived organizational performance. Proper implementation of post-selection HRM practices makes employee motivated that resulted in improved organizational performance. Management of Islamic banks desiring optimum performance should pay special attention to need-based training programs, merit-based performance appraisal system, development of progressive career paths, performance-based extrinsic and intrinsic rewards management. While making HRM policies, employee motivation should be given paramount importance as the results of contemporary studies supported that it is the major contributing factor of higher organizational performance.

  8. HRM in foreign-owned agricultural and food processing companies in Serbia

    Directory of Open Access Journals (Sweden)

    Ratković Tatjana

    2015-01-01

    Full Text Available This paper was aimed at analysing the degree to which foreign-owned agricultural and food processing companies in Serbia implement key human resource management (HRM processes, determining whether these processes are performed in-house or outsourced, and identifying the variables which influence the decision on performing these processes within the company or outsourcing. The analysis of data collected through a survey conducted in 42 companies has confirmed the expectations that the surveyed companies mainly perform most of HRM activities The conclusion drawn from the study is that all examined HRM activities are mainly performed in-house, rather than outsourced, while the mode of performing key HRM processes mainly depends on the size of the company and the number of employees in HR department, implying that foreign-owned companies operating in agricultural and food processing sector in this transition country need to take these variables into consideration when deciding whether to perform crucial HRM activities by themselves or outsource.

  9. EVALUATION OF METHODS FOR ESTIMATING FATIGUE PROPERTIES APPLIED TO STAINLESS STEELS AND ALUMINUM ALLOYS

    Directory of Open Access Journals (Sweden)

    Taylor Mac Intyer Fonseca Junior

    2013-12-01

    Full Text Available This work evaluate seven estimation methods of fatigue properties applied to stainless steels and aluminum alloys. Experimental strain-life curves are compared to the estimations obtained by each method. After applying seven different estimation methods at 14 material conditions, it was found that fatigue life can be estimated with good accuracy only by the Bäumel-Seeger method for the martensitic stainless steel tempered between 300°C and 500°C. The differences between mechanical behavior during monotonic and cyclic loading are probably the reason for the absence of a reliable method for estimation of fatigue behavior from monotonic properties for a group of materials.

  10. Applying the Mixed Methods Instrument Development and Construct Validation Process: the Transformative Experience Questionnaire

    Science.gov (United States)

    Koskey, Kristin L. K.; Sondergeld, Toni A.; Stewart, Victoria C.; Pugh, Kevin J.

    2018-01-01

    Onwuegbuzie and colleagues proposed the Instrument Development and Construct Validation (IDCV) process as a mixed methods framework for creating and validating measures. Examples applying IDCV are lacking. We provide an illustrative case integrating the Rasch model and cognitive interviews applied to the development of the Transformative…

  11. An Aural Learning Project: Assimilating Jazz Education Methods for Traditional Applied Pedagogy

    Science.gov (United States)

    Gamso, Nancy M.

    2011-01-01

    The Aural Learning Project (ALP) was developed to incorporate jazz method components into the author's classical practice and her applied woodwind lesson curriculum. The primary objective was to place a more focused pedagogical emphasis on listening and hearing than is traditionally used in the classical applied curriculum. The components of the…

  12. Complementary variational principle method applied to thermal conductivities of a plasma in a uniform magnetic field

    Energy Technology Data Exchange (ETDEWEB)

    Sehgal, A K; Gupta, S C [Punjabi Univ., Patiala (India). Dept. of Physics

    1982-12-14

    The complementary variational principles method (CVP) is applied to the thermal conductivities of a plasma in a uniform magnetic field. The results of computations show that the CVP derived results are very useful.

  13. Connecting empowerment-focused HRM and labor productivity to work engagement : The mediating role of job demands and resources

    NARCIS (Netherlands)

    van de Voorde, F.C.; Veld, M.; van Veldhoven, M.J.P.M.

    2016-01-01

    Integrating the strategic HRM literature with key aspects of the job demands-resources (JDR) model, we propose in this study that empowerment-focused HRM and labour productivity influence work engagement of employees by shaping task-related resources and demands. A total of 311 employees nested

  14. Connecting empowerment-focused HRM and labour productivity to work engagement: the mediating role of job demands and resources

    NARCIS (Netherlands)

    van de Voorde, Karina; Veld, M.F.A.; van Veldhoven, Marc

    2016-01-01

    Integrating the strategic HRM literature with key aspects of the job demands-resources (JDR) model, we propose in this study that empowerment-focused HRM and labour productivity influence work engagement of employees by shaping task-related resources and demands. A total of 311 employees nested

  15. Wielandt method applied to the diffusion equations discretized by finite element nodal methods

    International Nuclear Information System (INIS)

    Mugica R, A.; Valle G, E. del

    2003-01-01

    Nowadays the numerical methods of solution to the diffusion equation by means of algorithms and computer programs result so extensive due to the great number of routines and calculations that should carry out, this rebounds directly in the execution times of this programs, being obtained results in relatively long times. This work shows the application of an acceleration method of the convergence of the classic method of those powers that it reduces notably the number of necessary iterations for to obtain reliable results, what means that the compute times they see reduced in great measure. This method is known in the literature like Wielandt method and it has incorporated to a computer program that is based on the discretization of the neutron diffusion equations in plate geometry and stationary state by polynomial nodal methods. In this work the neutron diffusion equations are described for several energy groups and their discretization by means of those called physical nodal methods, being illustrated in particular the quadratic case. It is described a model problem widely described in the literature which is solved for the physical nodal grade schemes 1, 2, 3 and 4 in three different ways: to) with the classic method of the powers, b) method of the powers with the Wielandt acceleration and c) method of the powers with the Wielandt modified acceleration. The results for the model problem as well as for two additional problems known as benchmark problems are reported. Such acceleration method can also be implemented to problems of different geometry to the proposal in this work, besides being possible to extend their application to problems in 2 or 3 dimensions. (Author)

  16. What is the method in applying formal methods to PLC applications?

    NARCIS (Netherlands)

    Mader, Angelika H.; Engel, S.; Wupper, Hanno; Kowalewski, S.; Zaytoon, J.

    2000-01-01

    The question we investigate is how to obtain PLC applications with confidence in their proper functioning. Especially, we are interested in the contribution that formal methods can provide for their development. Our maxim is that the place of a particular formal method in the total picture of system

  17. The Value of e-HRM in Recruitment Strategies of Organizations

    DEFF Research Database (Denmark)

    Holm, Anna B.

    2014-01-01

    Although the online hiring process is regarded as being more cost efficient, and the fastest route to finding the right candidates, i.e. more effective than traditional paper-based recruitment, little is known how e-HRM in general and e-recruitment in particular impact the outcome of various...... the organization’s perspective, and then, drawing on existing literatures and previous research, to propose which consequences the integration of e-HRM and e-recruitment might have for the those strategies....

  18. Formal methods applied to industrial complex systems implementation of the B method

    CERN Document Server

    Boulanger, Jean-Louis

    2014-01-01

    This book presents real-world examples of formal techniques in an industrial context. It covers formal methods such as SCADE and/or the B Method, in various fields such as railways, aeronautics, and the automotive industry. The purpose of this book is to present a summary of experience on the use of "formal methods" (based on formal techniques such as proof, abstract interpretation and model-checking) in industrial examples of complex systems, based on the experience of people currently involved in the creation and assessment of safety critical system software. The involvement of people from

  19. A new clamp method for firing bricks | Obeng | Journal of Applied ...

    African Journals Online (AJOL)

    A new clamp method for firing bricks. ... Journal of Applied Science and Technology ... To overcome this operational deficiencies, a new method of firing bricks that uses brick clamp technique that incorporates a clamp wall of 60 cm thickness, a six tier approach of sealing the top of the clamp (by combination of green bricks) ...

  20. A method to evaluate performance reliability of individual subjects in laboratory research applied to work settings.

    Science.gov (United States)

    1978-10-01

    This report presents a method that may be used to evaluate the reliability of performance of individual subjects, particularly in applied laboratory research. The method is based on analysis of variance of a tasks-by-subjects data matrix, with all sc...

  1. Determination methods for plutonium as applied in the field of reprocessing

    International Nuclear Information System (INIS)

    1983-07-01

    The papers presented report on Pu-determination methods, which are routinely applied in process control, and also on new developments which could supercede current methods either because they are more accurate or because they are simpler and faster. (orig./DG) [de

  2. Water Permeability of Pervious Concrete Is Dependent on the Applied Pressure and Testing Methods

    Directory of Open Access Journals (Sweden)

    Yinghong Qin

    2015-01-01

    Full Text Available Falling head method (FHM and constant head method (CHM are, respectively, used to test the water permeability of permeable concrete, using different water heads on the testing samples. The results indicate the apparent permeability of pervious concrete decreasing with the applied water head. The results also demonstrate the permeability measured from the FHM is lower than that from the CHM. The fundamental difference between the CHM and FHM is examined from the theory of fluid flowing through porous media. The testing results suggest that the water permeability of permeable concrete should be reported with the applied pressure and the associated testing method.

  3. Accurate simulation of MPPT methods performance when applied to commercial photovoltaic panels.

    Science.gov (United States)

    Cubas, Javier; Pindado, Santiago; Sanz-Andrés, Ángel

    2015-01-01

    A new, simple, and quick-calculation methodology to obtain a solar panel model, based on the manufacturers' datasheet, to perform MPPT simulations, is described. The method takes into account variations on the ambient conditions (sun irradiation and solar cells temperature) and allows fast MPPT methods comparison or their performance prediction when applied to a particular solar panel. The feasibility of the described methodology is checked with four different MPPT methods applied to a commercial solar panel, within a day, and under realistic ambient conditions.

  4. Accurate Simulation of MPPT Methods Performance When Applied to Commercial Photovoltaic Panels

    Directory of Open Access Journals (Sweden)

    Javier Cubas

    2015-01-01

    Full Text Available A new, simple, and quick-calculation methodology to obtain a solar panel model, based on the manufacturers’ datasheet, to perform MPPT simulations, is described. The method takes into account variations on the ambient conditions (sun irradiation and solar cells temperature and allows fast MPPT methods comparison or their performance prediction when applied to a particular solar panel. The feasibility of the described methodology is checked with four different MPPT methods applied to a commercial solar panel, within a day, and under realistic ambient conditions.

  5. Diamond difference method with hybrid angular quadrature applied to neutron transport problems

    International Nuclear Information System (INIS)

    Zani, Jose H.; Barros, Ricardo C.; Alves Filho, Hermes

    2005-01-01

    In this work we presents the results for the calculations of the disadvantage factor in thermal nuclear reactor physics. We use the one-group discrete ordinates (S N ) equations to mathematically model the flux distributions in slab lattices. We apply the diamond difference method with source iteration iterative scheme to numerically solve the discretized systems equations. We used special interface conditions to describe the method with hybrid angular quadrature. We show numerical results to illustrate the accuracy of the hybrid method. (author)

  6. Proposal and Evaluation of Management Method for College Mechatronics Education Applying the Project Management

    Science.gov (United States)

    Ando, Yoshinobu; Eguchi, Yuya; Mizukawa, Makoto

    In this research, we proposed and evaluated a management method of college mechatronics education. We applied the project management to college mechatronics education. We practiced our management method to the seminar “Microcomputer Seminar” for 3rd grade students who belong to Department of Electrical Engineering, Shibaura Institute of Technology. We succeeded in management of Microcomputer Seminar in 2006. We obtained the good evaluation for our management method by means of questionnaire.

  7. Perspective for applying traditional and inovative teaching and learning methods to nurses continuing education

    OpenAIRE

    Bendinskaitė, Irmina

    2015-01-01

    Bendinskaitė I. Perspective for applying traditional and innovative teaching and learning methods to nurse’s continuing education, magister thesis / supervisor Assoc. Prof. O. Riklikienė; Departament of Nursing and Care, Faculty of Nursing, Lithuanian University of Health Sciences. – Kaunas, 2015, – p. 92 The purpose of this study was to investigate traditional and innovative teaching and learning methods perspective to nurse’s continuing education. Material and methods. In a period fro...

  8. Cluster detection methods applied to the Upper Cape Cod cancer data

    Directory of Open Access Journals (Sweden)

    Ozonoff David

    2005-09-01

    Full Text Available Abstract Background A variety of statistical methods have been suggested to assess the degree and/or the location of spatial clustering of disease cases. However, there is relatively little in the literature devoted to comparison and critique of different methods. Most of the available comparative studies rely on simulated data rather than real data sets. Methods We have chosen three methods currently used for examining spatial disease patterns: the M-statistic of Bonetti and Pagano; the Generalized Additive Model (GAM method as applied by Webster; and Kulldorff's spatial scan statistic. We apply these statistics to analyze breast cancer data from the Upper Cape Cancer Incidence Study using three different latency assumptions. Results The three different latency assumptions produced three different spatial patterns of cases and controls. For 20 year latency, all three methods generally concur. However, for 15 year latency and no latency assumptions, the methods produce different results when testing for global clustering. Conclusion The comparative analyses of real data sets by different statistical methods provides insight into directions for further research. We suggest a research program designed around examining real data sets to guide focused investigation of relevant features using simulated data, for the purpose of understanding how to interpret statistical methods applied to epidemiological data with a spatial component.

  9. Apparatus and method for applying an end plug to a fuel rod tube end

    International Nuclear Information System (INIS)

    Rieben, S.L.; Wylie, M.E.

    1987-01-01

    An apparatus is described for applying an end plug to a hollow end of a nuclear fuel rod tube, comprising: support means mounted for reciprocal movement between remote and adjacent positions relative to a nuclear fuel rod tube end to which an end plug is to be applied; guide means supported on the support means for movement; and drive means coupled to the support means and being actuatable for movement between retracted and extended positions for reciprocally moving the support means between its respective remote and adjacent positions. A method for applying an end plug to a hollow end of a nuclear fuel rod tube is also described

  10. Method of levelized discounted costs applied in economic evaluation of nuclear power plant project

    International Nuclear Information System (INIS)

    Tian Li; Wang Yongqing; Liu Jingquan; Guo Jilin; Liu Wei

    2000-01-01

    The main methods of economic evaluation of bid which are in common use are introduced. The characteristics of levelized discounted cost method and its application are presented. The method of levelized discounted cost is applied to the cost calculation of a 200 MW nuclear heating reactor economic evaluation. The results indicate that the method of levelized discounted costs is simple, feasible and which is considered most suitable for the economic evaluation of various case. The method is suggested which is used in the national economic evaluation

  11. Local regression type methods applied to the study of geophysics and high frequency financial data

    Science.gov (United States)

    Mariani, M. C.; Basu, K.

    2014-09-01

    In this work we applied locally weighted scatterplot smoothing techniques (Lowess/Loess) to Geophysical and high frequency financial data. We first analyze and apply this technique to the California earthquake geological data. A spatial analysis was performed to show that the estimation of the earthquake magnitude at a fixed location is very accurate up to the relative error of 0.01%. We also applied the same method to a high frequency data set arising in the financial sector and obtained similar satisfactory results. The application of this approach to the two different data sets demonstrates that the overall method is accurate and efficient, and the Lowess approach is much more desirable than the Loess method. The previous works studied the time series analysis; in this paper our local regression models perform a spatial analysis for the geophysics data providing different information. For the high frequency data, our models estimate the curve of best fit where data are dependent on time.

  12. Which HRM practices enhance employee outcomes at work across the life-span?

    NARCIS (Netherlands)

    Veth, Klaske; Korzilius, Hubert P.L.M.; van der Heijden, Beatrice I.J.M.; Emans, Ben; de Lange, Annet H.

    Based on the social exchange theory and on ageing and life-span theories, this paper aims to examine: (1) the relationships between perceived availability and use of HRM practices, and employee outcomes (i.e. work engagement and employability); and (2) how employee age moderates these relationships.

  13. Which HRM practices enhance employee outcomes at work across the life-span?

    NARCIS (Netherlands)

    Veth, K.N.; Korzilius, H.P.L.M.; Heijden, B.I.J.M. van der; Lange, A.H. de; Emans, B.J.M.

    2017-01-01

    Based on the social exchange theory and on ageing and life-span theories, this paper aims to examine: (1) the relationships between perceived availability and use of HRM practices, and employee outcomes (i.e. work engagement and employability); and (2) how employee age moderates these relationships.

  14. Influence of HRM Practices on Organizational Commitment: A Study among Software Professionals in India

    Science.gov (United States)

    Paul, A. K.; Anantharaman, R. N.

    2004-01-01

    Although organizational commitment has been discussed frequently in organizational psychology for almost four decades, few studies have involved software professionals. A study in India reveals that HRM practices such as employee-friendly work environment, career development, development oriented appraisal, and comprehensive training show a…

  15. HRM Practices in Public and Private Universities of Pakistan: A Comparative Study

    Science.gov (United States)

    Iqbal, Muhammad Zafar; Arif, Muhammad Irfan; Abbas, Furrakh

    2011-01-01

    The purpose of this study was to compare the HRM practices of public and private universities in Punjab province of Pakistan. The data for the study was collected through a questionnaire comprising 30 items mainly related to job definition, training and development, compensation, team work, employee's participation and performance appraisal. The…

  16. Leadership, knowledge and people in knowledge-intensive organisations : implications for HRM theory and practice

    NARCIS (Netherlands)

    Millar-Schijf, Carla C.J.M.; Chen, Stephen; Waller, Lee

    2017-01-01

    In order to offer insights into the management of HR challenges in Knowledge Intensive Firms/Organisations (KIO’s), this Special Issue explores three strands of research: International HRM, leadership and the knowledge-intensive firm/organisation. In this introduction against the background of

  17. HRM and strategic climates in hospitals: does the message come accross at the ward level?

    NARCIS (Netherlands)

    Veld, M.; Paauwe, J.; Boselie, J.P.P.E.F.

    2010-01-01

    This study examined how employees perceive intended strategic goals and HRM at the ward level, and if these perceptions generate the desired effects. The qualitative part of the research reveals that the hospital pursues two strategic goals (i.e. quality and safety). Analysis of the questionnaire

  18. Devolving HRM and HRD to Line managers: The Case of Hilton International's UK Hotels

    Science.gov (United States)

    Maxwell, Gillian; Watson, Sandra

    2004-01-01

    This paper explores line manager responsibility for human resource management (HRM) and human resource development (HRD) in an international hotel organisation. The results identify a strong support system for line managers in relation to training and good relations with HR specialists. However, less evident is senior management support coupled…

  19. Strategic Role of HRM in Turkey: A Three-Country Comparative Analysis

    Science.gov (United States)

    Ozcelik, Ayse Oya; Aydinli, Fulya

    2006-01-01

    Purpose: To explore the strategic role of human resource management (HRM) in Turkey by comparing Turkish companies to Spanish and German companies. Design/methodology/approach: The questionnaire form of the Cranet-G 1999-2000 Survey (Cranfield Network on Strategic International Human Resource Management) has been used to collect the data. The…

  20. Personeelsmanagement in China: een snelle transformatie van staatsgereguleerde personeelszorg naar modern HRM?

    NARCIS (Netherlands)

    de Boer, S.J.; Looise, Jan C.; Plomp, D.

    2003-01-01

    Personnel management in China: a rapid transformation from government controlled personnel care to modern HRM?China is one of few countries in the world that has been maintaining high economic growth rates even in current times economic decline. This has not gone unnoticed to Dutch business, whose

  1. HRM and its effect on employee, organizational and financial outcomes in health care organizations

    NARCIS (Netherlands)

    Vermeeren, B.; Steijn, A.J.; Tummers, L.G.; Lankhaar, M.; Poerstamper, R.J.; van Beek, S.

    2014-01-01

    Background One of the main goals of Human Resource Management (HRM) is to increase the performance of organizations. However, few studies have explicitly addressed the multidimensional character of performance and linked HR practices to various outcome dimensions. This study therefore adds to the

  2. HRM and its effect on employee, organizational and financial outcomes in health care organizations

    NARCIS (Netherlands)

    B. Vermeeren (Brenda); A.J. Steijn (Bram); L.G. Tummers (Lars); M. Lankhaar (Marcel); R.-J. Poerstamper (Robbert-Jan); S. van Beek (Sandra)

    2014-01-01

    markdownabstractBackground: One of the main goals of Human Resource Management (HRM) is to increase the performance of organizations. However, few studies have explicitly addressed the multidimensional character of performance and linked HR practices to various outcome dimensions. This study

  3. Employee Perceptions of HRM and TQM and the Effects on Satisfaction and INtention to Leave

    NARCIS (Netherlands)

    J.P.P.E.F. Boselie (Paul); A. van der Wiele (Ton)

    2001-01-01

    textabstractThere is a growing interest in theory and in practice with regard to the relationship between human resource management [HRM] and total quality management [TQM] as well as the relationship between these two perspectives and business performances. Empirical research suggests significant

  4. Method to detect substances in a body and device to apply the method

    International Nuclear Information System (INIS)

    Voigt, H.

    1978-01-01

    The method and the measuring disposition serve to localize pellets doped with Gd 2 O 3 , lying between UO 2 pellets within a reactor fuel rod. The fuel rod is penetrating a homogeneous magnetic field generated between two pole shoes. The magnetic stray field caused by the doping substances is then measured by means of Hall probes (e.g. InAs) for quantitative discrimination from UO 2 . The position of the Gd 2 O 3 -doped pellets is determined by moving the fuel rod through the magnetic field in a direction perpendicular to the homogeneous field. The measuring signal is caused by the different susceptibility of Gd 2 O 3 with respect to UO 2 . (DG) [de

  5. Criticality analysis of thermal reactors for two energy groups applying Monte Carlo and neutron Albedo method

    International Nuclear Information System (INIS)

    Terra, Andre Miguel Barge Pontes Torres

    2005-01-01

    The Albedo method applied to criticality calculations to nuclear reactors is characterized by following the neutron currents, allowing to make detailed analyses of the physics phenomena about interactions of the neutrons with the core-reflector set, by the determination of the probabilities of reflection, absorption, and transmission. Then, allowing to make detailed appreciations of the variation of the effective neutron multiplication factor, keff. In the present work, motivated for excellent results presented in dissertations applied to thermal reactors and shieldings, was described the methodology to Albedo method for the analysis criticality of thermal reactors by using two energy groups admitting variable core coefficients to each re-entrant current. By using the Monte Carlo KENO IV code was analyzed relation between the total fraction of neutrons absorbed in the core reactor and the fraction of neutrons that never have stayed into the reflector but were absorbed into the core. As parameters of comparison and analysis of the results obtained by the Albedo method were used one dimensional deterministic code ANISN (ANIsotropic SN transport code) and Diffusion method. The keff results determined by the Albedo method, to the type of analyzed reactor, showed excellent agreement. Thus were obtained relative errors of keff values smaller than 0,78% between the Albedo method and code ANISN. In relation to the Diffusion method were obtained errors smaller than 0,35%, showing the effectiveness of the Albedo method applied to criticality analysis. The easiness of application, simplicity and clarity of the Albedo method constitute a valuable instrument to neutronic calculations applied to nonmultiplying and multiplying media. (author)

  6. Applying electric field to charged and polar particles between metallic plates: extension of the Ewald method.

    Science.gov (United States)

    Takae, Kyohei; Onuki, Akira

    2013-09-28

    We develop an efficient Ewald method of molecular dynamics simulation for calculating the electrostatic interactions among charged and polar particles between parallel metallic plates, where we may apply an electric field with an arbitrary size. We use the fact that the potential from the surface charges is equivalent to the sum of those from image charges and dipoles located outside the cell. We present simulation results on boundary effects of charged and polar fluids, formation of ionic crystals, and formation of dipole chains, where the applied field and the image interaction are crucial. For polar fluids, we find a large deviation of the classical Lorentz-field relation between the local field and the applied field due to pair correlations along the applied field. As general aspects, we clarify the difference between the potential-fixed and the charge-fixed boundary conditions and examine the relationship between the discrete particle description and the continuum electrostatics.

  7. Non-invasive imaging methods applied to neo- and paleo-ontological cephalopod research

    Science.gov (United States)

    Hoffmann, R.; Schultz, J. A.; Schellhorn, R.; Rybacki, E.; Keupp, H.; Gerden, S. R.; Lemanis, R.; Zachow, S.

    2014-05-01

    Several non-invasive methods are common practice in natural sciences today. Here we present how they can be applied and contribute to current topics in cephalopod (paleo-) biology. Different methods will be compared in terms of time necessary to acquire the data, amount of data, accuracy/resolution, minimum/maximum size of objects that can be studied, the degree of post-processing needed and availability. The main application of the methods is seen in morphometry and volumetry of cephalopod shells. In particular we present a method for precise buoyancy calculation. Therefore, cephalopod shells were scanned together with different reference bodies, an approach developed in medical sciences. It is necessary to know the volume of the reference bodies, which should have similar absorption properties like the object of interest. Exact volumes can be obtained from surface scanning. Depending on the dimensions of the study object different computed tomography techniques were applied.

  8. Applying terminological methods and description logic for creating and implementing and ontology on inhibition

    DEFF Research Database (Denmark)

    Zambach, Sine; Madsen, Bodil Nistrup

    2009-01-01

    By applying formal terminological methods to model an ontology within the domain of enzyme inhibition, we aim to clarify concepts and to obtain consistency. Additionally, we propose a procedure for implementing this ontology in OWL with the aim of obtaining a strict structure which can form...

  9. Method of applying single higher order polynomial basis function over multiple domains

    CSIR Research Space (South Africa)

    Lysko, AA

    2010-03-01

    Full Text Available A novel method has been devised where one set of higher order polynomial-based basis functions can be applied over several wire segments, thus permitting to decouple the number of unknowns from the number of segments, and so from the geometrical...

  10. Applied probabilistic methods in the field of reactor safety in Germany

    International Nuclear Information System (INIS)

    Heuser, F.W.

    1982-01-01

    Some aspects of applied reliability and risk analysis methods in nuclear safety and the present role of both in Germany, are discussed. First, some comments on the status and applications of reliability analysis are given. Second, some conclusions that can be drawn from previous work on the German Risk Study are summarized. (orig.)

  11. 21 CFR 111.320 - What requirements apply to laboratory methods for testing and examination?

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false What requirements apply to laboratory methods for testing and examination? 111.320 Section 111.320 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION CURRENT GOOD MANUFACTURING...

  12. Splendor and misery of the distorted wave method applied to heavy ions transfer reactions

    International Nuclear Information System (INIS)

    Mermaz, M.C.

    1979-01-01

    The success and failure of the Distorted Wave Method (DWM) applied to heavy ion transfer reactions are illustrated by few examples: one and multi-nucleon transfer reactions induced by 15 N and 18 O on 28 Si target nucleus performed on the vicinity of Coulomb barrier respectively at 44 and 56 MeV incident energy

  13. A nodal method applied to a diffusion problem with generalized coefficients

    International Nuclear Information System (INIS)

    Laazizi, A.; Guessous, N.

    1999-01-01

    In this paper, we consider second order neutrons diffusion problem with coefficients in L ∞ (Ω). Nodal method of the lowest order is applied to approximate the problem's solution. The approximation uses special basis functions in which the coefficients appear. The rate of convergence obtained is O(h 2 ) in L 2 (Ω), with a free rectangular triangulation. (authors)

  14. Trends in Research Methods in Applied Linguistics: China and the West.

    Science.gov (United States)

    Yihong, Gao; Lichun, Li; Jun, Lu

    2001-01-01

    Examines and compares current trends in applied linguistics (AL) research methods in China and the West. Reviews AL articles in four Chinese journals, from 1978-1997, and four English journals from 1985 to 1997. Articles are categorized and subcategorized. Results show that in China, AL research is heading from non-empirical toward empirical, with…

  15. Critical path method applied to research project planning: Fire Economics Evaluation System (FEES)

    Science.gov (United States)

    Earl B. Anderson; R. Stanton Hales

    1986-01-01

    The critical path method (CPM) of network analysis (a) depicts precedence among the many activities in a project by a network diagram; (b) identifies critical activities by calculating their starting, finishing, and float times; and (c) displays possible schedules by constructing time charts. CPM was applied to the development of the Forest Service's Fire...

  16. Applying Activity Based Costing (ABC) Method to Calculate Cost Price in Hospital and Remedy Services.

    Science.gov (United States)

    Rajabi, A; Dabiri, A

    2012-01-01

    Activity Based Costing (ABC) is one of the new methods began appearing as a costing methodology in the 1990's. It calculates cost price by determining the usage of resources. In this study, ABC method was used for calculating cost price of remedial services in hospitals. To apply ABC method, Shahid Faghihi Hospital was selected. First, hospital units were divided into three main departments: administrative, diagnostic, and hospitalized. Second, activity centers were defined by the activity analysis method. Third, costs of administrative activity centers were allocated into diagnostic and operational departments based on the cost driver. Finally, with regard to the usage of cost objectives from services of activity centers, the cost price of medical services was calculated. The cost price from ABC method significantly differs from tariff method. In addition, high amount of indirect costs in the hospital indicates that capacities of resources are not used properly. Cost price of remedial services with tariff method is not properly calculated when compared with ABC method. ABC calculates cost price by applying suitable mechanisms but tariff method is based on the fixed price. In addition, ABC represents useful information about the amount and combination of cost price services.

  17. A new effective Monte Carlo Midway coupling method in MCNP applied to a well logging problem

    Energy Technology Data Exchange (ETDEWEB)

    Serov, I.V.; John, T.M.; Hoogenboom, J.E

    1998-12-01

    The background of the Midway forward-adjoint coupling method including the black absorber technique for efficient Monte Carlo determination of radiation detector responses is described. The method is implemented in the general purpose MCNP Monte Carlo code. The utilization of the method is fairly straightforward and does not require any substantial extra expertise. The method was applied to a standard neutron well logging porosity tool problem. The results exhibit reliability and high efficiency of the Midway method. For the studied problem the efficiency gain is considerably higher than for a normal forward calculation, which is already strongly optimized by weight-windows. No additional effort is required to adjust the Midway model if the position of the detector or the porosity of the formation is changed. Additionally, the Midway method can be used with other variance reduction techniques if extra gain in efficiency is desired.

  18. Determination of activity of I-125 applying sum-peak methods

    International Nuclear Information System (INIS)

    Arbelo Penna, Y.; Hernandez Rivero, A.T.; Oropesa Verdecia, P.; Serra Aguila, R.; Moreno Leon, Y.

    2011-01-01

    The determination of activity of I-125 in radioactive solutions, applying sum-peak methods, by using an n-type HPGe detector of extended range is described. Two procedures were used for obtaining I-125 specific activity in solutions: a) an absolute method, which is independent of nuclear parameters and detector efficiency, and b) an option which consider constant the efficiency in the region of interest and involves calculations using nuclear parameters. The measurement geometries studied are specifically solid point sources. The relative deviations between specific activities, obtained by these different procedures are not higher than 1 %. Moreover, the activity of the radioactive solution was obtained by measuring it in NIST ampoule using a CAPINTEC CRC 35R dose calibrator. The consistency of obtained results, confirm the feasibility of applying direct methods of measurement for I-125 activity determinations, which allow us to achieve lower uncertainties in comparison with the relative methods of measurement. The establishment of these methods is aimed to be applied for the calibration of equipment and radionuclide dose calibrators used currently in clinical RIA/IRMA assays and Nuclear medicine practice respectively. (Author)

  19. An applied study using systems engineering methods to prioritize green systems options

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Sonya M [Los Alamos National Laboratory; Macdonald, John M [Los Alamos National Laboratory

    2009-01-01

    For many years, there have been questions about the effectiveness of applying different green solutions. If you're building a home and wish to use green technologies, where do you start? While all technologies sound promising, which will perform the best over time? All this has to be considered within the cost and schedule of the project. The amount of information available on the topic can be overwhelming. We seek to examine if Systems Engineering methods can be used to help people choose and prioritize technologies that fit within their project and budget. Several methods are used to gain perspective into how to select the green technologies, such as the Analytic Hierarchy Process (AHP) and Kepner-Tregoe. In our study, subjects applied these methods to analyze cost, schedule, and trade-offs. Results will document whether the experimental approach is applicable to defining system priorities for green technologies.

  20. Economic consequences assessment for scenarios and actual accidents do the same methods apply

    International Nuclear Information System (INIS)

    Brenot, J.

    1991-01-01

    Methods for estimating the economic consequences of major technological accidents, and their corresponding computer codes, are briefly presented with emphasis on the basic choices. When applied to hypothetic scenarios, those methods give results that are of interest for risk managers with a decision aiding perspective. Simultaneously the various costs, and the procedures for their estimation are reviewed for some actual accidents (Three Mile Island, Chernobyl,..). These costs are used in a perspective of litigation and compensation. The comparison of the methods used and cost estimates obtained for scenarios and actual accidents shows the points of convergence and discrepancies that are discussed

  1. Non-invasive imaging methods applied to neo- and paleontological cephalopod research

    Science.gov (United States)

    Hoffmann, R.; Schultz, J. A.; Schellhorn, R.; Rybacki, E.; Keupp, H.; Gerden, S. R.; Lemanis, R.; Zachow, S.

    2013-11-01

    Several non-invasive methods are common practice in natural sciences today. Here we present how they can be applied and contribute to current topics in cephalopod (paleo-) biology. Different methods will be compared in terms of time necessary to acquire the data, amount of data, accuracy/resolution, minimum-maximum size of objects that can be studied, of the degree of post-processing needed and availability. Main application of the methods is seen in morphometry and volumetry of cephalopod shells in order to improve our understanding of diversity and disparity, functional morphology and biology of extinct and extant cephalopods.

  2. Covariance methodology applied to 35S disintegration rate measurements by the CIEMAT/NIST method

    International Nuclear Information System (INIS)

    Koskinas, M.F.; Nascimento, T.S.; Yamazaki, I.M.; Dias, M.S.

    2014-01-01

    The Nuclear Metrology Laboratory (LMN) at IPEN is carrying out measurements in a LSC (Liquid Scintillation Counting system), applying the CIEMAT/NIST method. In this context 35 S is an important radionuclide for medical applications and it is difficult to be standardized by other primary methods due to low beta ray energy. The CIEMAT/NIST is a standard technique used by most metrology laboratories in order to improve accuracy and speed up beta emitter standardization. The focus of the present work was to apply the covariance methodology for determining the overall uncertainty in the 35 S disintegration rate. All partial uncertainties involved in the measurements were considered, taking into account all possible correlations between each pair of them. - Highlights: ► 35 S disintegration rate measured in Liquid Scintillator system using CIEMAT/NIST method. ► Covariance methodology applied to the overall uncertainty in the 35 S disintegration rate. ► Monte Carlo simulation was applied to determine 35 S activity in the 4πβ(PC)-γ coincidence system

  3. Metabolic pathways of lung inflammation revealed by high-resolution metabolomics (HRM) of H1N1 influenza virus infection in mice.

    Science.gov (United States)

    Chandler, Joshua D; Hu, Xin; Ko, Eun-Ju; Park, Soojin; Lee, Young-Tae; Orr, Michael; Fernandes, Jolyn; Uppal, Karan; Kang, Sang-Moo; Jones, Dean P; Go, Young-Mi

    2016-11-01

    Influenza is a significant health concern worldwide. Viral infection induces local and systemic activation of the immune system causing attendant changes in metabolism. High-resolution metabolomics (HRM) uses advanced mass spectrometry and computational methods to measure thousands of metabolites inclusive of most metabolic pathways. We used HRM to identify metabolic pathways and clusters of association related to inflammatory cytokines in lungs of mice with H1N1 influenza virus infection. Infected mice showed progressive weight loss, decreased lung function, and severe lung inflammation with elevated cytokines [interleukin (IL)-1β, IL-6, IL-10, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ] and increased oxidative stress via cysteine oxidation. HRM showed prominent effects of influenza virus infection on tryptophan and other amino acids, and widespread effects on pathways including purines, pyrimidines, fatty acids, and glycerophospholipids. A metabolome-wide association study (MWAS) of the aforementioned inflammatory cytokines was used to determine the relationship of metabolic responses to inflammation during infection. This cytokine-MWAS (cMWAS) showed that metabolic associations consisted of distinct and shared clusters of 396 metabolites highly correlated with inflammatory cytokines. Strong negative associations of selected glycosphingolipid, linoleate, and tryptophan metabolites with IFN-γ contrasted strong positive associations of glycosphingolipid and bile acid metabolites with IL-1β, TNF-α, and IL-10. Anti-inflammatory cytokine IL-10 had strong positive associations with vitamin D, purine, and vitamin E metabolism. The detailed metabolic interactions with cytokines indicate that targeted metabolic interventions may be useful during life-threatening crises related to severe acute infection and inflammation. Copyright © 2016 the American Physiological Society.

  4. Genome Survey Sequencing of Luffa Cylindrica L. and Microsatellite High Resolution Melting (SSR-HRM) Analysis for Genetic Relationship of Luffa Genotypes.

    Science.gov (United States)

    An, Jianyu; Yin, Mengqi; Zhang, Qin; Gong, Dongting; Jia, Xiaowen; Guan, Yajing; Hu, Jin

    2017-09-11

    Luffa cylindrica (L.) Roem. is an economically important vegetable crop in China. However, the genomic information on this species is currently unknown. In this study, for the first time, a genome survey of L. cylindrica was carried out using next-generation sequencing (NGS) technology. In total, 43.40 Gb sequence data of L. cylindrica , about 54.94× coverage of the estimated genome size of 789.97 Mb, were obtained from HiSeq 2500 sequencing, in which the guanine plus cytosine (GC) content was calculated to be 37.90%. The heterozygosity of genome sequences was only 0.24%. In total, 1,913,731 contigs (>200 bp) with 525 bp N 50 length and 1,410,117 scaffolds (>200 bp) with 885.01 Mb total length were obtained. From the initial assembled L. cylindrica genome, 431,234 microsatellites (SSRs) (≥5 repeats) were identified. The motif types of SSR repeats included 62.88% di-nucleotide, 31.03% tri-nucleotide, 4.59% tetra-nucleotide, 0.96% penta-nucleotide and 0.54% hexa-nucleotide. Eighty genomic SSR markers were developed, and 51/80 primers could be used in both "Zheda 23" and "Zheda 83". Nineteen SSRs were used to investigate the genetic diversity among 32 accessions through SSR-HRM analysis. The unweighted pair group method analysis (UPGMA) dendrogram tree was built by calculating the SSR-HRM raw data. SSR-HRM could be effectively used for genotype relationship analysis of Luffa species.

  5. Power System Oscillation Modes Identifications: Guidelines for Applying TLS-ESPRIT Method

    Science.gov (United States)

    Gajjar, Gopal R.; Soman, Shreevardhan

    2013-05-01

    Fast measurements of power system quantities available through wide-area measurement systems enables direct observations for power system electromechanical oscillations. But the raw observations data need to be processed to obtain the quantitative measures required to make any inference regarding the power system state. A detailed discussion is presented for the theory behind the general problem of oscillatory mode indentification. This paper presents some results on oscillation mode identification applied to a wide-area frequency measurements system. Guidelines for selection of parametes for obtaining most reliable results from the applied method are provided. Finally, some results on real measurements are presented with our inference on them.

  6. Multigrid method applied to the solution of an elliptic, generalized eigenvalue problem

    Energy Technology Data Exchange (ETDEWEB)

    Alchalabi, R.M. [BOC Group, Murray Hill, NJ (United States); Turinsky, P.J. [North Carolina State Univ., Raleigh, NC (United States)

    1996-12-31

    The work presented in this paper is concerned with the development of an efficient MG algorithm for the solution of an elliptic, generalized eigenvalue problem. The application is specifically applied to the multigroup neutron diffusion equation which is discretized by utilizing the Nodal Expansion Method (NEM). The underlying relaxation method is the Power Method, also known as the (Outer-Inner Method). The inner iterations are completed using Multi-color Line SOR, and the outer iterations are accelerated using Chebyshev Semi-iterative Method. Furthermore, the MG algorithm utilizes the consistent homogenization concept to construct the restriction operator, and a form function as a prolongation operator. The MG algorithm was integrated into the reactor neutronic analysis code NESTLE, and numerical results were obtained from solving production type benchmark problems.

  7. Least Square NUFFT Methods Applied to 2D and 3D Radially Encoded MR Image Reconstruction

    Science.gov (United States)

    Song, Jiayu; Liu, Qing H.; Gewalt, Sally L.; Cofer, Gary; Johnson, G. Allan

    2009-01-01

    Radially encoded MR imaging (MRI) has gained increasing attention in applications such as hyperpolarized gas imaging, contrast-enhanced MR angiography, and dynamic imaging, due to its motion insensitivity and improved artifact properties. However, since the technique collects k-space samples nonuniformly, multidimensional (especially 3D) radially sampled MRI image reconstruction is challenging. The balance between reconstruction accuracy and speed becomes critical when a large data set is processed. Kaiser-Bessel gridding reconstruction has been widely used for non-Cartesian reconstruction. The objective of this work is to provide an alternative reconstruction option in high dimensions with on-the-fly kernels calculation. The work develops general multi-dimensional least square nonuniform fast Fourier transform (LS-NUFFT) algorithms and incorporates them into a k-space simulation and image reconstruction framework. The method is then applied to reconstruct the radially encoded k-space, although the method addresses general nonuniformity and is applicable to any non-Cartesian patterns. Performance assessments are made by comparing the LS-NUFFT based method with the conventional Kaiser-Bessel gridding method for 2D and 3D radially encoded computer simulated phantoms and physically scanned phantoms. The results show that the LS-NUFFT reconstruction method has better accuracy-speed efficiency than the Kaiser-Bessel gridding method when the kernel weights are calculated on the fly. The accuracy of the LS-NUFFT method depends on the choice of scaling factor, and it is found that for a particular conventional kernel function, using its corresponding deapodization function as scaling factor and utilizing it into the LS-NUFFT framework has the potential to improve accuracy. When a cosine scaling factor is used, in particular, the LS-NUFFT method is faster than Kaiser-Bessel gridding method because of a quasi closed-form solution. The method is successfully applied to 2D and

  8. Detection of sdhB Gene Mutations in SDHI-Resistant Isolates of Botrytis cinerea Using High Resolution Melting (HRM) Analysis.

    Science.gov (United States)

    Samaras, Anastasios; Madesis, Panagiotis; Karaoglanidis, George S

    2016-01-01

    Botrytis cinerea , is a high risk pathogen for fungicide resistance development. Pathogen' resistance to SDHIs is associated with several mutations in sdh gene. The diversity of mutations and their differential effect on cross-resistance patterns among SDHIs and the fitness of resistant strains necessitate the availability of a tool for their rapid identification. This study was initiated to develop and validate a high-resolution melting (HRM) analysis for the identification of P225H/F/L//T, N230I, and H272L/R/Y mutations. Based on the sequence of sdh B subunit of resistant and sensitive isolates, a universal primer pair was designed. The specificity of the HRM analysis primers was verified to ensure against the cross-reaction with other fungal species and its sensitivity was evaluated using concentrations of known amounts of mutant's DNA. The melting curve analysis generated nine distinct curve profiles, enabling the discrimination of all the four mutations located at codon 225, the N230I mutation, the three mutations located in codon 272, and the non-mutated isolates (isolates of wild-type sensitivity). Similar results were obtained when DNA was extracted directly from artificially inoculated strawberry fruit. The method was validated by monitoring the presence of sdh B mutations in samples of naturally infected strawberry fruits and stone fruit rootstock seedling plants showing damping-off symptoms. HRM analysis data were compared with a standard PIRA-PCR technique and an absolute agreement was observed suggesting that in both populations the H272R mutation was the predominant one, while H272Y, N230I, and P225H were detected in lower frequencies. The results of the study suggest that HRM analysis can be a useful tool for sensate, accurate, and rapid identification of several sdh B mutations in B. cinerea and it is expected to contribute in routine fungicide resistance monitoring or assessments of the effectiveness of anti-resistance strategies implemented in

  9. Detection of sdhB gene mutations in SDHI-resistant isolates of Botrytis cinerea using high resolution melting (HRM analysis

    Directory of Open Access Journals (Sweden)

    Anastasios Samaras

    2016-11-01

    Full Text Available Botrytis cinerea, is a high-risk pathogen for fungicide resistance development. Pathogen` resistance to SDHIs is associated with several mutations in sdh gene. The diversity of mutations and their differential effect on cross-resistance patterns among SDHIs and the fitness of resistant strains necessitate the availability of a tool for their rapid identification. This study was initiated to develop and validate a high-resolution melting (HRM analysis for the identification of P225H/F/L//T, N230I and H272L/R/Y mutations. Based on the sequence of sdhB subunit of resistant and sensitive isolates, a universal primer pair was designed. The specificity of the HRM analysis primers was verified to ensure against the cross-reaction with other fungal species and its sensitivity was evaluated using concentrations of known amounts of mutant`s DNA. The melting curve analysis generated nine distinct curve profiles, enabling the discrimination of all the 4 mutations located at codon 225, the N230I mutation, the 3 mutations located in codon 272 and the non mutated isolates (isolates of wild type sensitivity. Similar results were obtained when DNA was extracted directly from artificially inoculated strawberry fruit. The method was validated by monitoring the presence of sdhB mutations in samples of naturally infected strawberry fruits and stone fruit rootstock seedling plants showing damping off symptoms. HRM analysis data were compared with a standard PIRA-PCR technique and an absolute agreement was observed suggesting that in both populations the H272R mutation was the predominant one, while H272Y, N230I and P225H were detected in lower frequencies. The results of the study suggest that HRM analysis can be a useful tool for sensate, accurate and rapid identification of several sdhB mutations in B. cinerea and it is expected to contribute in routine fungicide resistance monitoring or assessments of the effectiveness of antiresistance strategies implemented in

  10. Applying Mathematical Optimization Methods to an ACT-R Instance-Based Learning Model.

    Science.gov (United States)

    Said, Nadia; Engelhart, Michael; Kirches, Christian; Körkel, Stefan; Holt, Daniel V

    2016-01-01

    Computational models of cognition provide an interface to connect advanced mathematical tools and methods to empirically supported theories of behavior in psychology, cognitive science, and neuroscience. In this article, we consider a computational model of instance-based learning, implemented in the ACT-R cognitive architecture. We propose an approach for obtaining mathematical reformulations of such cognitive models that improve their computational tractability. For the well-established Sugar Factory dynamic decision making task, we conduct a simulation study to analyze central model parameters. We show how mathematical optimization techniques can be applied to efficiently identify optimal parameter values with respect to different optimization goals. Beyond these methodological contributions, our analysis reveals the sensitivity of this particular task with respect to initial settings and yields new insights into how average human performance deviates from potential optimal performance. We conclude by discussing possible extensions of our approach as well as future steps towards applying more powerful derivative-based optimization methods.

  11. Development of a two-step high-resolution melting (HRM) analysis for screening sequence variants associated with resistance to the QoIs, benzimidazoles and dicarboximides in airborne inoculum of Botrytis cinerea.

    Science.gov (United States)

    Chatzidimopoulos, Michael; Ganopoulos, Ioannis; Vellios, Evangelos; Madesis, Panagiotis; Tsaftaris, Athanasios; Pappas, Athanassios C

    2014-11-01

    A rapid, high-resolution melting (HRM) analysis protocol was developed to detect sequence variations associated with resistance to the QoIs, benzimidazoles and dicarboximides in Botrytis cinerea airborne inoculum. HRM analysis was applied directly in fungal DNA collected from air samplers with selective medium. Three and five different genotypes were detected and classified according to their melting profiles in BenA and bos1 genes associated with resistance to benzimidazoles and dicarboximides, respectively. The sensitivity of the methodology was evident in the case of the QoIs, where genotypes varying either by a single nucleotide polymorphism or an additional 1205-bp intron were separated accurately with a single pair of primers. The developed two-step protocol was completed in 82 min and showed reduced variation in the melting curves' formation. HRM analysis rapidly detected the major mutations found in greenhouse strains providing accurate data for successfully controlling grey mould. © 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.

  12. Applied ecosystem analysis - a primer; the ecosystem diagnosis and treatment method

    International Nuclear Information System (INIS)

    Lestelle, L.C.; Mobrand, L.E.; Lichatowich, J.A.; Vogel, T.S.

    1996-05-01

    The aim of this document is to inform and instruct the reader about an approach to ecosystem management that is based upon salmon as an indicator species. It is intended to provide natural resource management professionals with the background information needed to answer questions about why and how to apply the approach. The methods and tools the authors describe are continually updated and refined, so this primer should be treated as a first iteration of a sequentially revised manual

  13. An Ultrasonic Guided Wave Method to Estimate Applied Biaxial Loads (Preprint)

    Science.gov (United States)

    2011-11-01

    VALIDATION A fatigue test was performed with an array of six surface-bonded PZT transducers on a 6061 aluminum plate as shown in Figure 4. The specimen...direct paths of propagation are oriented at different angles. This method is applied to experimental sparse array data recorded during a fatigue test...and the additional complication of the resulting fatigue cracks interfering with some of the direct arrivals is addressed via proper selection of

  14. Accuracy of the Adomian decomposition method applied to the Lorenz system

    International Nuclear Information System (INIS)

    Hashim, I.; Noorani, M.S.M.; Ahmad, R.; Bakar, S.A.; Ismail, E.S.; Zakaria, A.M.

    2006-01-01

    In this paper, the Adomian decomposition method (ADM) is applied to the famous Lorenz system. The ADM yields an analytical solution in terms of a rapidly convergent infinite power series with easily computable terms. Comparisons between the decomposition solutions and the fourth-order Runge-Kutta (RK4) numerical solutions are made for various time steps. In particular we look at the accuracy of the ADM as the Lorenz system changes from a non-chaotic system to a chaotic one

  15. Applying the Delphi method to assess impacts of forest management on biodiversity and habitat preservation

    DEFF Research Database (Denmark)

    Filyushkina, Anna; Strange, Niels; Löf, Magnus

    2018-01-01

    This study applied a structured expert elicitation technique, the Delphi method, to identify the impacts of five forest management alternatives and several forest characteristics on the preservation of biodiversity and habitats in the boreal zone of the Nordic countries. The panel of experts...... as a valuable addition to on-going empirical and modeling efforts. The findings could assist forest managers in developing forest management strategies that generate benefits from timber production while taking into account the trade-offs with biodiversity goals....

  16. Modified Method of Simplest Equation Applied to the Nonlinear Schrödinger Equation

    Directory of Open Access Journals (Sweden)

    Vitanov Nikolay K.

    2018-03-01

    Full Text Available We consider an extension of the methodology of the modified method of simplest equation to the case of use of two simplest equations. The extended methodology is applied for obtaining exact solutions of model nonlinear partial differential equations for deep water waves: the nonlinear Schrödinger equation. It is shown that the methodology works also for other equations of the nonlinear Schrödinger kind.

  17. Modified Method of Simplest Equation Applied to the Nonlinear Schrödinger Equation

    Science.gov (United States)

    Vitanov, Nikolay K.; Dimitrova, Zlatinka I.

    2018-03-01

    We consider an extension of the methodology of the modified method of simplest equation to the case of use of two simplest equations. The extended methodology is applied for obtaining exact solutions of model nonlinear partial differential equations for deep water waves: the nonlinear Schrödinger equation. It is shown that the methodology works also for other equations of the nonlinear Schrödinger kind.

  18. Applied Ecosystem Analysis - - a Primer : EDT the Ecosystem Diagnosis and Treatment Method.

    Energy Technology Data Exchange (ETDEWEB)

    Lestelle, Lawrence C.; Mobrand, Lars E.

    1996-05-01

    The aim of this document is to inform and instruct the reader about an approach to ecosystem management that is based upon salmon as an indicator species. It is intended to provide natural resource management professionals with the background information needed to answer questions about why and how to apply the approach. The methods and tools the authors describe are continually updated and refined, so this primer should be treated as a first iteration of a sequentially revised manual.

  19. The LTSN method used in transport equation, applied in nuclear engineering problems

    International Nuclear Information System (INIS)

    Borges, Volnei; Vilhena, Marco Tulio de

    2002-01-01

    The LTS N method solves analytically the S N equations, applying the Laplace transform in the spatial variable. This methodology is used in determination of scalar flux for neutrons and photons, absorbed dose rate, buildup factors and power for a heterogeneous planar slab. This procedure leads to the solution of a transcendental equations for effective multiplication, critical thickness and the atomic density. In this work numerical results are reported, considering multigroup problem in heterogeneous slab. (author)

  20. Machine Learning Method Applied in Readout System of Superheated Droplet Detector

    Science.gov (United States)

    Liu, Yi; Sullivan, Clair Julia; d'Errico, Francesco

    2017-07-01

    Direct readability is one advantage of superheated droplet detectors in neutron dosimetry. Utilizing such a distinct characteristic, an imaging readout system analyzes image of the detector for neutron dose readout. To improve the accuracy and precision of algorithms in the imaging readout system, machine learning algorithms were developed. Deep learning neural network and support vector machine algorithms are applied and compared with generally used Hough transform and curvature analysis methods. The machine learning methods showed a much higher accuracy and better precision in recognizing circular gas bubbles.

  1. Translation Methods Applied in Translating Quotations in “the Secret” by Rhonda

    OpenAIRE

    FEBRIANTI, VICKY

    2014-01-01

    Keywords: Translation Methods, The Secret, Quotations.Translation helps human to get information written in any language evenwhen it is written in foreign languages. Therefore translation happens in printed media. Books have been popular printed media. The Secret written by Rhonda Byrne is a popular self-help book which has been translated into 50 languages including Indonesian (“The Secret”, n.d., para.5-6).This study is meant to find out the translation methods applied in The Secret. The wr...

  2. Development of a tracking method for augmented reality applied to nuclear plant maintenance work

    International Nuclear Information System (INIS)

    Shimoda, Hiroshi; Maeshima, Masayuki; Nakai, Toshinori; Bian, Zhiqiang; Ishii, Hirotake; Yoshikawa, Hidekazu

    2005-01-01

    In this paper, a plant maintenance support method is described, which employs the state-of-the-art information technology, Augmented Reality (AR), in order to improve efficiency of NPP maintenance work and to prevent from human error. Although AR has a great possibility to support various works in real world, it is difficult to apply it to actual work support because the tracking method is the bottleneck for the practical use. In this study, a bar code marker tracking method is proposed to apply AR system for a maintenance work support in NPP field. The proposed method calculates the users position and orientation in real time by two long markers, which are captured by the user-mounted camera. The markers can be easily pasted on the pipes in plant field, and they can be easily recognized in long distance in order to reduce the number of pasted markers in the work field. Experiments were conducted in a laboratory and plant field to evaluate the proposed method. The results show that (1) fast and stable tracking can be realized, (2) position error in camera view is less than 1%, which is almost perfect under the limitation of camera resolution, and (3) it is relatively difficult to catch two markers in one camera view especially in short distance

  3. Applying the response matrix method for solving coupled neutron diffusion and transport problems

    International Nuclear Information System (INIS)

    Sibiya, G.S.

    1980-01-01

    The numerical determination of the flux and power distribution in the design of large power reactors is quite a time-consuming procedure if the space under consideration is to be subdivided into very fine weshes. Many computing methods applied in reactor physics (such as the finite-difference method) require considerable computing time. In this thesis it is shown that the response matrix method can be successfully used as an alternative approach to solving the two-dimension diffusion equation. Furthermore it is shown that sufficient accuracy of the method is achieved by assuming a linear space dependence of the neutron currents on the boundaries of the geometries defined for the given space. (orig.) [de

  4. Method to integrate clinical guidelines into the electronic health record (EHR) by applying the archetypes approach.

    Science.gov (United States)

    Garcia, Diego; Moro, Claudia Maria Cabral; Cicogna, Paulo Eduardo; Carvalho, Deborah Ribeiro

    2013-01-01

    Clinical guidelines are documents that assist healthcare professionals, facilitating and standardizing diagnosis, management, and treatment in specific areas. Computerized guidelines as decision support systems (DSS) attempt to increase the performance of tasks and facilitate the use of guidelines. Most DSS are not integrated into the electronic health record (EHR), ordering some degree of rework especially related to data collection. This study's objective was to present a method for integrating clinical guidelines into the EHR. The study developed first a way to identify data and rules contained in the guidelines, and then incorporate rules into an archetype-based EHR. The proposed method tested was anemia treatment in the Chronic Kidney Disease Guideline. The phases of the method are: data and rules identification; archetypes elaboration; rules definition and inclusion in inference engine; and DSS-EHR integration and validation. The main feature of the proposed method is that it is generic and can be applied toany type of guideline.

  5. Lessons learned applying CASE methods/tools to Ada software development projects

    Science.gov (United States)

    Blumberg, Maurice H.; Randall, Richard L.

    1993-01-01

    This paper describes the lessons learned from introducing CASE methods/tools into organizations and applying them to actual Ada software development projects. This paper will be useful to any organization planning to introduce a software engineering environment (SEE) or evolving an existing one. It contains management level lessons learned, as well as lessons learned in using specific SEE tools/methods. The experiences presented are from Alpha Test projects established under the STARS (Software Technology for Adaptable and Reliable Systems) project. They reflect the front end efforts by those projects to understand the tools/methods, initial experiences in their introduction and use, and later experiences in the use of specific tools/methods and the introduction of new ones.

  6. Applying some methods to process the data coming from the nuclear reactions

    International Nuclear Information System (INIS)

    Suleymanov, M.K.; Abdinov, O.B.; Belashev, B.Z.

    2010-01-01

    Full text : The methods of a posterior increasing the resolution of the spectral lines are offered to process the data coming from the nuclear reactions. The methods have applied to process the data coming from the nuclear reactions at high energies. They give possibilities to get more detail information on a structure of the spectra of particles emitted in the nuclear reactions. The nuclear reactions are main source of the information on the structure and physics of the atomic nuclei. Usually the spectrums of the fragments of the reactions are complex ones. Apparently it is not simple to extract the necessary for investigation information. In the talk we discuss the methods of a posterior increasing the resolution of the spectral lines. The methods could be useful to process the complex data coming from the nuclear reactions. We consider the Fourier transformation method and maximum entropy one. The complex structures were identified by the method. One can see that at lest two selected points are indicated by the method. Recent we presented a talk where we shown that the results of the analyzing the structure of the pseudorapidity spectra of charged relativistic particles with ≥ 0.7 measured in Au+Em and Pb+Em at AGS and SPS energies using the Fourier transformation method and maximum entropy one. The dependences of these spectra on the number of fast target protons were studied. These distribution shown visually some plateau and shoulder that was at least three selected points on the distributions. The plateaus become wider in PbEm reactions. The existing of plateau is necessary for the parton models. The maximum entropy method could confirm the existing of the plateau and the shoulder on the distributions. The figure shows the results of applying the maximum entropy method. One can see that the method indicates several clean selected points. Some of them same with observed visually ones. We would like to note that the Fourier transformation method could not

  7. Rapid detection of pathological mutations and deletions of the haemoglobin beta gene (HBB) by High Resolution Melting (HRM) analysis and Gene Ratio Analysis Copy Enumeration PCR (GRACE-PCR).

    Science.gov (United States)

    Turner, Andrew; Sasse, Jurgen; Varadi, Aniko

    2016-10-19

    Inherited disorders of haemoglobin are the world's most common genetic diseases, resulting in significant morbidity and mortality. The large number of mutations associated with the haemoglobin beta gene (HBB) makes gene scanning by High Resolution Melting (HRM) PCR an attractive diagnostic approach. However, existing HRM-PCR assays are not able to detect all common point mutations and have only a very limited ability to detect larger gene rearrangements. The aim of the current study was to develop a HBB assay, which can be used as a screening test in highly heterogeneous populations, for detection of both point mutations and larger gene rearrangements. The assay is based on a combination of conventional HRM-PCR and a novel Gene Ratio Analysis Copy Enumeration (GRACE) PCR method. HRM-PCR was extensively optimised, which included the use of an unlabelled probe and incorporation of universal bases into primers to prevent interference from common non-pathological polymorphisms. GRACE-PCR was employed to determine HBB gene copy numbers relative to a reference gene using melt curve analysis to detect rearrangements in the HBB gene. The performance of the assay was evaluated by analysing 410 samples. A total of 44 distinct pathological genotypes were detected. In comparison with reference methods, the assay has a sensitivity of 100 % and a specificity of 98 %. We have developed an assay that detects both point mutations and larger rearrangements of the HBB gene. This assay is quick, sensitive, specific and cost effective making it suitable as an initial screening test that can be used for highly heterogeneous cohorts.

  8. Detection of Dirofilaria immitis and other arthropod-borne filarioids by an HRM real-time qPCR, blood-concentrating techniques and a serological assay in dogs from Costa Rica.

    Science.gov (United States)

    Rojas, Alicia; Rojas, Diana; Montenegro, Víctor M; Baneth, Gad

    2015-03-23

    Canine filarioids are important nematodes transmitted to dogs by arthropods. Diagnosis of canine filariosis is accomplished by the microscopic identification of microfilariae, serology or PCR for filarial-DNA. The aim of this study was to evaluate a molecular assay for the detection of canine filariae in dog blood, to compare its performance to other diagnostic techniques, and to determine the relationship between microfilarial concentration and infection with other vector-borne pathogens. Blood samples from 146 dogs from Costa Rica were subjected to the detection of canine filarioids by four different methods: the microhematocrit tube test (MCT), Knott's modified test, serology and a high resolution melt and quantitative real-time PCR (HRM-qPCR). Co-infection with other vector-borne pathogens was also evaluated. Fifteen percent of the dogs were positive to Dirofilaria immitis by at least one of the methods. The HRM-qPCR produced distinctive melting plots for the different filarial worms and revealed that 11.6% of dogs were infected with Acanthocheilonema reconditum. The latter assay had a limit of detection of 2.4x10⁻⁴ mf/μl and detected infections with lower microfilarial concentrations in comparison to the microscopic techniques and the serological assay. The MCT and Knott's test only detected dogs with D. immitis microfilaremias above 0.7 mf/μl. Nevertheless, there was a strong correlation between the microfilarial concentration obtained by the Knott's modified test and the HRM-qPCR (r = 0.906, p HRM-qPCR showed the most sensitive and reliable performance in the detection of blood filaroids in comparison to the Knott's modified test, the MCT test and a serological assay.

  9. Strategic HR? A Study of the Perceived Role of HRM Departments in Brazil and Peru

    Directory of Open Access Journals (Sweden)

    Roberto Coda

    2009-01-01

    Full Text Available This study discusses the perceived role of the Human Resource Management Department and its perceived capacity of outstanding performance by comparing data collected in Brazil and Peru from employees of large organizations as well as participants in MBA programs at renowned schools. The non-probabilistic sample was composed of 416 Brazilian and 90 Peruvian respondents. The results point out that both in Brazil and Peru, the relevance attached to the HRM Department role for contributing to the future success of organizations does not correspond to its current capacity of performance. As such, in these realities the HRM Department has not yet made the qualitative leap that would enable its strategic role in organizations.

  10. Intestinal colic in newborn babies: incidence and methods of proceeding applied by parents

    Directory of Open Access Journals (Sweden)

    Anna Lewandowska

    2017-06-01

    Full Text Available Introduction: Intestinal colic is one of the more frequent complaints that a general practitioner and paediatrician deal with in their work. 10-40% of babies formula fed and 10-20% breast fed are stricken by this complaint. A colic attack appears suddenly and very quickly causes energetic, squeaky cry or even scream. Colic attacks last for a few minutes and appear every 2-3 hours usually in the evenings. Specialist literature provides numerous definitions of intestinal colic. The concept was introduced for the first time to paediatric textbooks over 250 years ago. One of the most accurate definitions describe colic as recurring attacks of intensive cry and anxiety lasting for more than 3 hours a day, 3 days a week within 3 weeks. Care of a baby suffering from an intestinal colic causes numerous problems and anxiety among parents, therefore knowledge of effective methods to combat this complaint is a challenge for contemporary neonatology and paediatrics. The aim of the study is to estimate the incidence of intestinal colic in newborn babies formula and breast fed as well as to assess methods of proceeding applied by parents and analyze their effectiveness. Material and methods: The research involved 100 newborn babies breast fed and 100 formula fed, and their parents. The research method applied in the study was a diagnostic survey conducted by use of a questionnaire method. Results: Among examined newborn babies that were breast fed, 43% have experienced intestinal colic, while among those formula fed 30% have suffered from it. The study involved 44% new born female babies and 56% male babies. 52% of mothers were 30-34 years old, 30% 35-59 years old, and 17% 25-59 years old. When it comes to families, the most numerous was a group in good financial situation (60%. The second numerous group was that in average financial situation (40%. All the respondents claimed that they had the knowledge on intestinal colic and the main source of knowledge

  11. Should methods of correction for multiple comparisons be applied in pharmacovigilance?

    Directory of Open Access Journals (Sweden)

    Lorenza Scotti

    2015-12-01

    Full Text Available Purpose. In pharmacovigilance, spontaneous reporting databases are devoted to the early detection of adverse event ‘signals’ of marketed drugs. A common limitation of these systems is the wide number of concurrently investigated associations, implying a high probability of generating positive signals simply by chance. However it is not clear if the application of methods aimed to adjust for the multiple testing problems are needed when at least some of the drug-outcome relationship under study are known. To this aim we applied a robust estimation method for the FDR (rFDR particularly suitable in the pharmacovigilance context. Methods. We exploited the data available for the SAFEGUARD project to apply the rFDR estimation methods to detect potential false positive signals of adverse reactions attributable to the use of non-insulin blood glucose lowering drugs. Specifically, the number of signals generated from the conventional disproportionality measures and after the application of the rFDR adjustment method was compared. Results. Among the 311 evaluable pairs (i.e., drug-event pairs with at least one adverse event report, 106 (34% signals were considered as significant from the conventional analysis. Among them 1 resulted in false positive signals according to rFDR method. Conclusions. The results of this study seem to suggest that when a restricted number of drug-outcome pairs is considered and warnings about some of them are known, multiple comparisons methods for recognizing false positive signals are not so useful as suggested by theoretical considerations.

  12. Solution of the neutron point kinetics equations with temperature feedback effects applying the polynomial approach method

    Energy Technology Data Exchange (ETDEWEB)

    Tumelero, Fernanda, E-mail: fernanda.tumelero@yahoo.com.br [Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS (Brazil). Programa de Pos-Graduacao em Engenharia Mecanica; Petersen, Claudio Z.; Goncalves, Glenio A.; Lazzari, Luana, E-mail: claudiopeteren@yahoo.com.br, E-mail: gleniogoncalves@yahoo.com.br, E-mail: luana-lazzari@hotmail.com [Universidade Federal de Pelotas (DME/UFPEL), Capao do Leao, RS (Brazil). Instituto de Fisica e Matematica

    2015-07-01

    In this work, we present a solution of the Neutron Point Kinetics Equations with temperature feedback effects applying the Polynomial Approach Method. For the solution, we consider one and six groups of delayed neutrons precursors with temperature feedback effects and constant reactivity. The main idea is to expand the neutron density, delayed neutron precursors and temperature as a power series considering the reactivity as an arbitrary function of the time in a relatively short time interval around an ordinary point. In the first interval one applies the initial conditions of the problem and the analytical continuation is used to determine the solutions of the next intervals. With the application of the Polynomial Approximation Method it is possible to overcome the stiffness problem of the equations. In such a way, one varies the time step size of the Polynomial Approach Method and performs an analysis about the precision and computational time. Moreover, we compare the method with different types of approaches (linear, quadratic and cubic) of the power series. The answer of neutron density and temperature obtained by numerical simulations with linear approximation are compared with results in the literature. (author)

  13. Power secant method applied to natural frequency extraction of Timoshenko beam structures

    Directory of Open Access Journals (Sweden)

    C.A.N. Dias

    Full Text Available This work deals with an improved plane frame formulation whose exact dynamic stiffness matrix (DSM presents, uniquely, null determinant for the natural frequencies. In comparison with the classical DSM, the formulation herein presented has some major advantages: local mode shapes are preserved in the formulation so that, for any positive frequency, the DSM will never be ill-conditioned; in the absence of poles, it is possible to employ the secant method in order to have a more computationally efficient eigenvalue extraction procedure. Applying the procedure to the more general case of Timoshenko beams, we introduce a new technique, named "power deflation", that makes the secant method suitable for the transcendental nonlinear eigenvalue problems based on the improved DSM. In order to avoid overflow occurrences that can hinder the secant method iterations, limiting frequencies are formulated, with scaling also applied to the eigenvalue problem. Comparisons with results available in the literature demonstrate the strength of the proposed method. Computational efficiency is compared with solutions obtained both by FEM and by the Wittrick-Williams algorithm.

  14. Solution of the neutron point kinetics equations with temperature feedback effects applying the polynomial approach method

    International Nuclear Information System (INIS)

    Tumelero, Fernanda; Petersen, Claudio Z.; Goncalves, Glenio A.; Lazzari, Luana

    2015-01-01

    In this work, we present a solution of the Neutron Point Kinetics Equations with temperature feedback effects applying the Polynomial Approach Method. For the solution, we consider one and six groups of delayed neutrons precursors with temperature feedback effects and constant reactivity. The main idea is to expand the neutron density, delayed neutron precursors and temperature as a power series considering the reactivity as an arbitrary function of the time in a relatively short time interval around an ordinary point. In the first interval one applies the initial conditions of the problem and the analytical continuation is used to determine the solutions of the next intervals. With the application of the Polynomial Approximation Method it is possible to overcome the stiffness problem of the equations. In such a way, one varies the time step size of the Polynomial Approach Method and performs an analysis about the precision and computational time. Moreover, we compare the method with different types of approaches (linear, quadratic and cubic) of the power series. The answer of neutron density and temperature obtained by numerical simulations with linear approximation are compared with results in the literature. (author)

  15. A methodological framework applied to the choice of the best method in replacement of nuclear systems

    International Nuclear Information System (INIS)

    Vianna Filho, Alfredo Marques

    2009-01-01

    The economic equipment replacement problem is a central question in Nuclear Engineering. On the one hand, new equipment are more attractive given their best performance, better reliability, lower maintenance cost etc. New equipment, however, require a higher initial investment. On the other hand, old equipment represent the other way around, with lower performance, lower reliability and specially higher maintenance costs, but in contrast having lower financial and insurance costs. The weighting of all these costs can be made with deterministic and probabilistic methods applied to the study of equipment replacement. Two types of distinct problems will be examined, substitution imposed by the wearing and substitution imposed by the failures. In order to solve the problem of nuclear system substitution imposed by wearing, deterministic methods are discussed. In order to solve the problem of nuclear system substitution imposed by failures, probabilistic methods are discussed. The aim of this paper is to present a methodological framework to the choice of the most useful method applied in the problem of nuclear system substitution.(author)

  16. Inclusive HRM and employment security for disabled people: An interdisciplinary approach

    OpenAIRE

    Borghouts - van de Pas, Irmgard; Freese, Charissa

    2017-01-01

    Many countries struggle with non-participation of vulnerable groups. Countries give priority to Active Labour Market Policies (ALMP) to help unemployed and disabled people get back to work, preferably in regular employment. In recent years, some innovative policies have tried to involve employers. There seems to be a paradox between desired social policy outcomes, such as getting people out of social security into regular jobs, and preferred Strategic HRM outcomes, such as being a highly prod...

  17. The Impact of Different HRM Regimes on Labour Productivity: National Results and a Regional Perspective

    OpenAIRE

    Hodgkinson, Ann

    2002-01-01

    This paper uses AWIRS 95 and IRWIRS 96-7 data to test whether workplaces which used ‘soft’ versus ‘hard’ Human Resource Management (HRM) policies and practices experienced significant differences in labour productivity improvements. Generally, the results support the proposition that management attitudes, policies and practices which aim to develop workforce skills, commitment and motivation were positively associated with improvements in labour productivity. Very few ‘hard’ practices other t...

  18. Exploring the ideological undercurrents of HRM: Workplace values and beliefs in Ireland & New Zealand

    OpenAIRE

    Geare, Alan; Edgar, Fiona; Harney, Brian; Cafferkey, Kenneth; Dundon, Tony

    2014-01-01

    Despite hints of more pluralist undercurrents, workplace values and beliefs have rarely been surfaced to inform our understanding of HRM. This paper examines management and employee workplace values and beliefs in the national contexts of Ireland and New Zealand. The findings indicate a) a divergence of managerial beliefs at the level of society and the level of their own workplace, b) an overall pluralist orientation amongst employees. These findings highlight the importance of greater sensi...

  19. An investigation of the effects of technology readiness on technology acceptance in e-HRM

    OpenAIRE

    Erdoğmuş, Nihat; Esen, Murat

    2011-01-01

    The aim of this paper is to investigate the effects of technology readiness on technology acceptance in e-HRM field. The data for this study were collected from 65 Human Resource (HR) managers representing top 500 largest private sector companies in Turkey. The research model based on two theories: Parasuraman's technology readiness and Davis’ technology acceptance model. The results of the study showed that optimism and innovativeness dimensions of technology readiness positively influenced ...

  20. HRM and its effect on employee, organizational and financial outcomes in health care organizations

    OpenAIRE

    Vermeeren, Brenda; Steijn, Bram; Tummers, Lars; Lankhaar, Marcel; Poerstamper, Robbert-Jan; Beek, Sandra

    2014-01-01

    markdownabstractBackground: One of the main goals of Human Resource Management (HRM) is to increase the performance of organizations. However, few studies have explicitly addressed the multidimensional character of performance and linked HR practices to various outcome dimensions. This study therefore adds to the literature by relating HR practices to three outcome dimensions: financial, organizational and employee (HR) outcomes. Furthermore, we will analyze how HR practices influence these o...

  1. HRM and its effect on employee, organizational and financial outcomes in health care organizations.

    Science.gov (United States)

    Vermeeren, Brenda; Steijn, Bram; Tummers, Lars; Lankhaar, Marcel; Poerstamper, Robbert-Jan; van Beek, Sandra

    2014-06-17

    One of the main goals of Human Resource Management (HRM) is to increase the performance of organizations. However, few studies have explicitly addressed the multidimensional character of performance and linked HR practices to various outcome dimensions. This study therefore adds to the literature by relating HR practices to three outcome dimensions: financial, organizational and employee (HR) outcomes. Furthermore, we will analyze how HR practices influence these outcome dimensions, focusing on the mediating role of job satisfaction. This study uses a unique dataset, based on the 'ActiZ Benchmark in Healthcare', a benchmark study conducted in Dutch home care, nursing care and care homes. Data from autumn 2010 to autumn 2011 were analyzed. In total, 162 organizations participated during this period (approximately 35% of all Dutch care organizations). Employee data were collected using a questionnaire (61,061 individuals, response rate 42%). Clients were surveyed using the Client Quality Index for long-term care, via stratified sampling. Financial outcomes were collected using annual reports. SEM analyses were conducted to test the hypotheses. It was found that HR practices are - directly or indirectly - linked to all three outcomes. The use of HR practices is related to improved financial outcomes (measure: net margin), organizational outcomes (measure: client satisfaction) and HR outcomes (measure: sickness absence). The impact of HR practices on HR outcomes and organizational outcomes proved substantially larger than their impact on financial outcomes. Furthermore, with respect to HR and organizational outcomes, the hypotheses concerning the full mediating effect of job satisfaction are confirmed. This is in line with the view that employee attitudes are an important element in the 'black box' between HRM and performance. The results underscore the importance of HRM in the health care sector, especially for HR and organizational outcomes. Further analyses of HRM

  2. Employee commitment in MNCs: impacts of organizational culture, HRM and top management orientations

    OpenAIRE

    Taylor, Sully; Levy, Orly; Beechler, Schon; Boyacıgiller, Nakiye Avdan; Boyacigiller, Nakiye Avdan

    2006-01-01

    This paper examines the impact of organizational culture and HRM system on employee commitment of core employees in multinational companies (MNCs). In addition, it identifies two top management team orientations global orientation and geocentric orientation that are seen as contributing uniquely to employee commitment in international firms. We found strong overall support for the model. The results also suggest that High Performance Work Practices have a positive impact on commitment r...

  3. Solution and study of nodal neutron transport equation applying the LTSN-DiagExp method

    International Nuclear Information System (INIS)

    Hauser, Eliete Biasotto; Pazos, Ruben Panta; Vilhena, Marco Tullio de; Barros, Ricardo Carvalho de

    2003-01-01

    In this paper we report advances about the three-dimensional nodal discrete-ordinates approximations of neutron transport equation for Cartesian geometry. We use the combined collocation method of the angular variables and nodal approach for the spatial variables. By nodal approach we mean the iterated transverse integration of the S N equations. This procedure leads to the set of one-dimensional averages angular fluxes in each spatial variable. The resulting system of equations is solved with the LTS N method, first applying the Laplace transform to the set of the nodal S N equations and then obtained the solution by symbolic computation. We include the LTS N method by diagonalization to solve the nodal neutron transport equation and then we outline the convergence of these nodal-LTS N approximations with the help of a norm associated to the quadrature formula used to approximate the integral term of the neutron transport equation. (author)

  4. Artificial intelligence methods applied for quantitative analysis of natural radioactive sources

    International Nuclear Information System (INIS)

    Medhat, M.E.

    2012-01-01

    Highlights: ► Basic description of artificial neural networks. ► Natural gamma ray sources and problem of detections. ► Application of neural network for peak detection and activity determination. - Abstract: Artificial neural network (ANN) represents one of artificial intelligence methods in the field of modeling and uncertainty in different applications. The objective of the proposed work was focused to apply ANN to identify isotopes and to predict uncertainties of their activities of some natural radioactive sources. The method was tested for analyzing gamma-ray spectra emitted from natural radionuclides in soil samples detected by a high-resolution gamma-ray spectrometry based on HPGe (high purity germanium). The principle of the suggested method is described, including, relevant input parameters definition, input data scaling and networks training. It is clear that there is satisfactory agreement between obtained and predicted results using neural network.

  5. Scalable Methods for Eulerian-Lagrangian Simulation Applied to Compressible Multiphase Flows

    Science.gov (United States)

    Zwick, David; Hackl, Jason; Balachandar, S.

    2017-11-01

    Multiphase flows can be found in countless areas of physics and engineering. Many of these flows can be classified as dispersed two-phase flows, meaning that there are solid particles dispersed in a continuous fluid phase. A common technique for simulating such flow is the Eulerian-Lagrangian method. While useful, this method can suffer from scaling issues on larger problem sizes that are typical of many realistic geometries. Here we present scalable techniques for Eulerian-Lagrangian simulations and apply it to the simulation of a particle bed subjected to expansion waves in a shock tube. The results show that the methods presented here are viable for simulation of larger problems on modern supercomputers. This material is based upon work supported by the National Science Foundation Graduate Research Fellowship under Grant No. DGE-1315138. This work was supported in part by the U.S. Department of Energy under Contract No. DE-NA0002378.

  6. Relativistic convergent close-coupling method applied to electron scattering from mercury

    International Nuclear Information System (INIS)

    Bostock, Christopher J.; Fursa, Dmitry V.; Bray, Igor

    2010-01-01

    We report on the extension of the recently formulated relativistic convergent close-coupling (RCCC) method to accommodate two-electron and quasi-two-electron targets. We apply the theory to electron scattering from mercury and obtain differential and integrated cross sections for elastic and inelastic scattering. We compared with previous nonrelativistic convergent close-coupling (CCC) calculations and for a number of transitions obtained significantly better agreement with the experiment. The RCCC method is able to resolve structure in the integrated cross sections for the energy regime in the vicinity of the excitation thresholds for the (6s6p) 3 P 0,1,2 states. These cross sections are associated with the formation of negative ion (Hg - ) resonances that could not be resolved with the nonrelativistic CCC method. The RCCC results are compared with the experiment and other relativistic theories.

  7. A reflective lens: applying critical systems thinking and visual methods to ecohealth research.

    Science.gov (United States)

    Cleland, Deborah; Wyborn, Carina

    2010-12-01

    Critical systems methodology has been advocated as an effective and ethical way to engage with the uncertainty and conflicting values common to ecohealth problems. We use two contrasting case studies, coral reef management in the Philippines and national park management in Australia, to illustrate the value of critical systems approaches in exploring how people respond to environmental threats to their physical and spiritual well-being. In both cases, we used visual methods--participatory modeling and rich picturing, respectively. The critical systems methodology, with its emphasis on reflection, guided an appraisal of the research process. A discussion of these two case studies suggests that visual methods can be usefully applied within a critical systems framework to offer new insights into ecohealth issues across a diverse range of socio-political contexts. With this article, we hope to open up a conversation with other practitioners to expand the use of visual methods in integrated research.

  8. ECONOMIC IMPLICATIONS OF E-HRM SYSTEM IMPLEMENTATION IN DEDEMAN COMPANY

    Directory of Open Access Journals (Sweden)

    Sofia Totolici

    2013-12-01

    Full Text Available Due to the situation in recent times and the latest global economic trends, an idea presented decades ago was reiterated: the most important resource of any organization is its human capital. As a direct consequence of this known idea, many specialists have treated in recent years, subjects which were related in one way or another with the field of Human Resources, since Human Resources sector will survive in the new economy only if it has a computer technology. As a result, the essential condition for achieving excellence in the computerized management of human resources management is the development and implementation of performant e-HRM systems. For this reason, any organization, regardless of its size and nature, must resort to the implementation of an e-HRM information system adapted to its specific needs, because the performance of the human resources activities (HR depend to a large extent on the correlation between software instruments and employees’ skills. In order to identify the effects and benefits of implementing e-HRM systems, we presented a case study focused on Dedeman Company – retailer of construction materials and interior design.

  9. Numerical consideration for multiscale statistical process control method applied to nuclear material accountancy

    International Nuclear Information System (INIS)

    Suzuki, Mitsutoshi; Hori, Masato; Asou, Ryoji; Usuda, Shigekazu

    2006-01-01

    The multiscale statistical process control (MSSPC) method is applied to clarify the elements of material unaccounted for (MUF) in large scale reprocessing plants using numerical calculations. Continuous wavelet functions are used to decompose the process data, which simulate batch operation superimposed by various types of disturbance, and the disturbance components included in the data are divided into time and frequency spaces. The diagnosis of MSSPC is applied to distinguish abnormal events from the process data and shows how to detect abrupt and protracted diversions using principle component analysis. Quantitative performance of MSSPC for the time series data is shown with average run lengths given by Monte-Carlo simulation to compare to the non-detection probability β. Recent discussion about bias corrections in material balances is introduced and another approach is presented to evaluate MUF without assuming the measurement error model. (author)

  10. The reduction method of statistic scale applied to study of climatic change

    International Nuclear Information System (INIS)

    Bernal Suarez, Nestor Ricardo; Molina Lizcano, Alicia; Martinez Collantes, Jorge; Pabon Jose Daniel

    2000-01-01

    In climate change studies the global circulation models of the atmosphere (GCMAs) enable one to simulate the global climate, with the field variables being represented on a grid points 300 km apart. One particular interest concerns the simulation of possible changes in rainfall and surface air temperature due to an assumed increase of greenhouse gases. However, the models yield the climatic projections on grid points that in most cases do not correspond to the sites of major interest. To achieve local estimates of the climatological variables, methods like the one known as statistical down scaling are applied. In this article we show a case in point by applying canonical correlation analysis (CCA) to the Guajira Region in the northeast of Colombia

  11. Parallel Implicit Runge-Kutta Methods Applied to Coupled Orbit/Attitude Propagation

    Science.gov (United States)

    Hatten, Noble; Russell, Ryan P.

    2017-12-01

    A variable-step Gauss-Legendre implicit Runge-Kutta (GLIRK) propagator is applied to coupled orbit/attitude propagation. Concepts previously shown to improve efficiency in 3DOF propagation are modified and extended to the 6DOF problem, including the use of variable-fidelity dynamics models. The impact of computing the stage dynamics of a single step in parallel is examined using up to 23 threads and 22 associated GLIRK stages; one thread is reserved for an extra dynamics function evaluation used in the estimation of the local truncation error. Efficiency is found to peak for typical examples when using approximately 8 to 12 stages for both serial and parallel implementations. Accuracy and efficiency compare favorably to explicit Runge-Kutta and linear-multistep solvers for representative scenarios. However, linear-multistep methods are found to be more efficient for some applications, particularly in a serial computing environment, or when parallelism can be applied across multiple trajectories.

  12. ADVANTAGES AND DISADVANTAGES OF APPLYING EVOLVED METHODS IN MANAGEMENT ACCOUNTING PRACTICE

    Directory of Open Access Journals (Sweden)

    SABOU FELICIA

    2014-05-01

    Full Text Available The evolved methods of management accounting have been developed with the purpose of removing the disadvantages of the classical methods, they are methods adapted to the new market conditions, which provide much more useful cost-related information so that the management of the company is able to take certain strategic decisions. Out of the category of evolved methods, the most used is the one of standard-costs due to the advantages that it presents, being used widely in calculating the production costs in some developed countries. The main advantages of the standard-cost method are: in-advance knowledge of the production costs and the measures that ensure compliance to these; with the help of the deviations calculated from the standard costs, one manages a systematic control over the costs, thus allowing the making of decision in due time, in as far as the elimination of the deviations and the improvement of the activity are concerned and it is a method of analysis, control and cost forecast; Although the advantages of using standards are significant, there are a few disadvantages to the employment of the standard-cost method: sometimes there can appear difficulties in establishing the deviations from the standard costs, the method does not allow an accurate calculation of the fixed costs. As a result of the study, we can observe the fact that the evolved methods of management accounting, as compared to the classical ones, present a series of advantages linked to a better analysis, control, and foreseeing of costs, whereas the main disadvantage is related to the large amount of work necessary for these methods to be applied.

  13. ADVANTAGES AND DISADVANTAGES OF APPLYING EVOLVED METHODS IN MANAGEMENT ACCOUNTING PRACTICE

    Directory of Open Access Journals (Sweden)

    SABOU FELICIA

    2014-05-01

    Full Text Available The evolved methods of management accounting have been developed with the purpose of removing the disadvantages of the classical methods, they are methods adapted to the new market conditions, which provide much more useful cost-related information so that the management of the company is able to take certain strategic decisions. Out of the category of evolved methods, the most used is the one of standard-costs due to the advantages that it presents, being used widely in calculating the production costs in some developed countries. The main advantages of the standard-cost method are: in-advance knowledge of the production costs and the measures that ensure compliance to these; with the help of the deviations calculated from the standard costs, one manages a systematic control over the costs, thus allowing the making of decision in due time, in as far as the elimination of the deviations and the improvement of the activity are concerned and it is a method of analysis, control and cost forecast; Although the advantages of using standards are significant, there are a few disadvantages to the employment of the standard-cost method: sometimes there can appear difficulties in establishing the deviations from the standard costs, the method does not allow an accurate calculation of the fixed costs. As a result of the study, we can observe the fact that the evolved methods of management accounting, as compared to the classical ones, present a series of advantages linked to a better analysis, control, and foreseeing of costs, whereas the main disadvantage is related to the large amount of work necessary for these methods to be applied

  14. The Fractional Step Method Applied to Simulations of Natural Convective Flows

    Science.gov (United States)

    Westra, Douglas G.; Heinrich, Juan C.; Saxon, Jeff (Technical Monitor)

    2002-01-01

    This paper describes research done to apply the Fractional Step Method to finite-element simulations of natural convective flows in pure liquids, permeable media, and in a directionally solidified metal alloy casting. The Fractional Step Method has been applied commonly to high Reynold's number flow simulations, but is less common for low Reynold's number flows, such as natural convection in liquids and in permeable media. The Fractional Step Method offers increased speed and reduced memory requirements by allowing non-coupled solution of the pressure and the velocity components. The Fractional Step Method has particular benefits for predicting flows in a directionally solidified alloy, since other methods presently employed are not very efficient. Previously, the most suitable method for predicting flows in a directionally solidified binary alloy was the penalty method. The penalty method requires direct matrix solvers, due to the penalty term. The Fractional Step Method allows iterative solution of the finite element stiffness matrices, thereby allowing more efficient solution of the matrices. The Fractional Step Method also lends itself to parallel processing, since the velocity component stiffness matrices can be built and solved independently of each other. The finite-element simulations of a directionally solidified casting are used to predict macrosegregation in directionally solidified castings. In particular, the finite-element simulations predict the existence of 'channels' within the processing mushy zone and subsequently 'freckles' within the fully processed solid, which are known to result from macrosegregation, or what is often referred to as thermo-solutal convection. These freckles cause material property non-uniformities in directionally solidified castings; therefore many of these castings are scrapped. The phenomenon of natural convection in an alloy under-going directional solidification, or thermo-solutal convection, will be explained. The

  15. A Rapid Coordinate Transformation Method Applied in Industrial Robot Calibration Based on Characteristic Line Coincidence

    Science.gov (United States)

    Liu, Bailing; Zhang, Fumin; Qu, Xinghua; Shi, Xiaojia

    2016-01-01

    Coordinate transformation plays an indispensable role in industrial measurements, including photogrammetry, geodesy, laser 3-D measurement and robotics. The widely applied methods of coordinate transformation are generally based on solving the equations of point clouds. Despite the high accuracy, this might result in no solution due to the use of ill conditioned matrices. In this paper, a novel coordinate transformation method is proposed, not based on the equation solution but based on the geometric transformation. We construct characteristic lines to represent the coordinate systems. According to the space geometry relation, the characteristic line scan is made to coincide by a series of rotations and translations. The transformation matrix can be obtained using matrix transformation theory. Experiments are designed to compare the proposed method with other methods. The results show that the proposed method has the same high accuracy, but the operation is more convenient and flexible. A multi-sensor combined measurement system is also presented to improve the position accuracy of a robot with the calibration of the robot kinematic parameters. Experimental verification shows that the position accuracy of robot manipulator is improved by 45.8% with the proposed method and robot calibration. PMID:26901203

  16. Resonating group method as applied to the spectroscopy of α-transfer reactions

    Science.gov (United States)

    Subbotin, V. B.; Semjonov, V. M.; Gridnev, K. A.; Hefter, E. F.

    1983-10-01

    In the conventional approach to α-transfer reactions the finite- and/or zero-range distorted-wave Born approximation is used in liaison with a macroscopic description of the captured α particle in the residual nucleus. Here the specific example of 16O(6Li,d)20Ne reactions at different projectile energies is taken to present a microscopic resonating group method analysis of the α particle in the final nucleus (for the reaction part the simple zero-range distorted-wave Born approximation is employed). In the discussion of suitable nucleon-nucleon interactions, force number one of the effective interactions presented by Volkov is shown to be most appropriate for the system considered. Application of the continuous analog of Newton's method to the evaluation of the resonating group method equations yields an increased accuracy with respect to traditional methods. The resonating group method description induces only minor changes in the structures of the angular distributions, but it does serve its purpose in yielding reliable and consistent spectroscopic information. NUCLEAR STRUCTURE 16O(6Li,d)20Ne; E=20 to 32 MeV; calculated B(E2); reduced widths, dσdΩ extracted α-spectroscopic factors. ZRDWBA with microscope RGM description of residual α particle in 20Ne; application of continuous analog of Newton's method; tested and applied Volkov force No. 1; direct mechanism.

  17. A Rapid Coordinate Transformation Method Applied in Industrial Robot Calibration Based on Characteristic Line Coincidence

    Directory of Open Access Journals (Sweden)

    Bailing Liu

    2016-02-01

    Full Text Available Coordinate transformation plays an indispensable role in industrial measurements, including photogrammetry, geodesy, laser 3-D measurement and robotics. The widely applied methods of coordinate transformation are generally based on solving the equations of point clouds. Despite the high accuracy, this might result in no solution due to the use of ill conditioned matrices. In this paper, a novel coordinate transformation method is proposed, not based on the equation solution but based on the geometric transformation. We construct characteristic lines to represent the coordinate systems. According to the space geometry relation, the characteristic line scan is made to coincide by a series of rotations and translations. The transformation matrix can be obtained using matrix transformation theory. Experiments are designed to compare the proposed method with other methods. The results show that the proposed method has the same high accuracy, but the operation is more convenient and flexible. A multi-sensor combined measurement system is also presented to improve the position accuracy of a robot with the calibration of the robot kinematic parameters. Experimental verification shows that the position accuracy of robot manipulator is improved by 45.8% with the proposed method and robot calibration.

  18. The Inverse System Method Applied to the Derivation of Power System Non—linear Control Laws

    Institute of Scientific and Technical Information of China (English)

    DonghaiLI; XuezhiJIANG; 等

    1997-01-01

    The differential geometric method has been applied to a series of power system non-linear control problems effectively.However a set of differential equations must be solved for obtaining the required diffeomorphic transformation.Therefore the derivation of control laws is very complicated.In fact because of the specificity of power system models the required diffeomorphic transformation may be obtained directly,so it is unnecessary to solve a set of differential equations.In addition inverse system method is equivalent to differential geometric method in reality and not limited to affine nonlinear systems,Its physical meaning is able to be viewed directly and its deduction needs only algebraic operation and derivation,so control laws can be obtained easily and the application to engineering is very convenient.Authors of this paper take steam valving control of power system as a typical case to be studied.It is demonstrated that the control law deduced by inverse system method is just the same as one by differential geometric method.The conclusion will simplify the control law derivations of steam valving,excitation,converter and static var compensator by differential geometric method and may be suited to similar control problems in other areas.

  19. Comparison of Heuristic Methods Applied for Optimal Operation of Water Resources

    Directory of Open Access Journals (Sweden)

    Alireza Borhani Dariane

    2009-01-01

    Full Text Available Water resources optimization problems are usually complex and hard to solve using the ordinary optimization methods, or they are at least  not economically efficient. A great number of studies have been conducted in quest of suitable methods capable of handling such problems. In recent years, some new heuristic methods such as genetic and ant algorithms have been introduced in systems engineering. Preliminary applications of these methods in water resources problems have shown that some of them are powerful tools, capable of solving complex problems. In this paper, the application of such heuristic methods as Genetic Algorithm (GA and Ant Colony Optimization (ACO have been studied for optimizing reservoir operation. The Dez Dam reservoir inIranwas chosen for a case study. The methods were applied and compared using short-term (one year and long-term models. Comparison of the results showed that GA outperforms both DP and ACO in finding true global optimum solutions and operating rules.

  20. Boundary element methods applied to two-dimensional neutron diffusion problems

    International Nuclear Information System (INIS)

    Itagaki, Masafumi

    1985-01-01

    The Boundary element method (BEM) has been applied to two-dimensional neutron diffusion problems. The boundary integral equation and its discretized form have been derived. Some numerical techniques have been developed, which can be applied to critical and fixed-source problems including multi-region ones. Two types of test programs have been developed according to whether the 'zero-determinant search' or the 'source iteration' technique is adopted for criticality search. Both programs require only the fluxes and currents on boundaries as the unknown variables. The former allows a reduction in computing time and memory in comparison with the finite element method (FEM). The latter is not always efficient in terms of computing time due to the domain integral related to the inhomogeneous source term; however, this domain integral can be replaced by the equivalent boundary integral for a region with a non-multiplying medium or with a uniform source, resulting in a significant reduction in computing time. The BEM, as well as the FEM, is well suited for solving irregular geometrical problems for which the finite difference method (FDM) is unsuited. The BEM also solves problems with infinite domains, which cannot be solved by the ordinary FEM and FDM. Some simple test calculations are made to compare the BEM with the FEM and FDM, and discussions are made concerning the relative merits of the BEM and problems requiring future solution. (author)

  1. Methodical basis of training of cadets for the military applied heptathlon competitions

    Directory of Open Access Journals (Sweden)

    R.V. Anatskyi

    2017-12-01

    Full Text Available The purpose of the research is to develop methodical bases of training of cadets for the military applied heptathlon competitions. Material and methods: Cadets of 2-3 courses at the age of 19-20 years (n=20 participated in researches. Cadets were selected by the best results of exercises performing included into the program of military applied heptathlon competitions (100 m run, 50 m freestyle swimming, Kalashnikov rifle shooting, pull-up, obstacle course, grenade throwing, 3000 m run. Preparation took place on the basis of training center. All trainings were organized and carried out according to the methodical basics: in a week preparation microcycle five days cadets had two trainings a day (on Saturday was one training, on Sunday they had rest. The selected exercises with individual loads were performed, Results : Sport scores demonstrated top results in the performance of 100 m run, 3000 m run and pull-up. The indices of performing exercise "obstacle course" were much lower than expected. Rather low results were demonstrated in swimming and shooting. Conclusions . Results of researches indicate the necessity of quality improvement: cadets’ weapons proficiency; physical readiness to perform the exercises requiring complex demonstration of all physical qualities.

  2. Nutrient Runoff Losses from Liquid Dairy Manure Applied with Low-Disturbance Methods.

    Science.gov (United States)

    Jokela, William; Sherman, Jessica; Cavadini, Jason

    2016-09-01

    Manure applied to cropland is a source of phosphorus (P) and nitrogen (N) in surface runoff and can contribute to impairment of surface waters. Tillage immediately after application incorporates manure into the soil, which may reduce nutrient loss in runoff as well as N loss via NH volatilization. However, tillage also incorporates crop residue, which reduces surface cover and may increase erosion potential. We applied liquid dairy manure in a silage corn ( L.)-cereal rye ( L.) cover crop system in late October using methods designed to incorporate manure with minimal soil and residue disturbance. These include strip-till injection and tine aerator-band manure application, which were compared with standard broadcast application, either incorporated with a disk or left on the surface. Runoff was generated with a portable rainfall simulator (42 mm h for 30 min) three separate times: (i) 2 to 5 d after the October manure application, (ii) in early spring, and (iii) after tillage and planting. In the postmanure application runoff, the highest losses of total P and dissolved reactive P were from surface-applied manure. Dissolved P loss was reduced 98% by strip-till injection; this result was not statistically different from the no-manure control. Reductions from the aerator band method and disk incorporation were 53 and 80%, respectively. Total P losses followed a similar pattern, with 87% reduction from injected manure. Runoff losses of N had generally similar patterns to those of P. Losses of P and N were, in most cases, lower in the spring rain simulations with fewer significant treatment effects. Overall, results show that low-disturbance manure application methods can significantly reduce nutrient runoff losses compared with surface application while maintaining residue cover better than incorporation by tillage. Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.

  3. Validation of methylation-sensitive high-resolution melting (MS-HRM) for the detection of stool DNA methylation in colorectal neoplasms.

    Science.gov (United States)

    Xiao, Zhujun; Li, Bingsheng; Wang, Guozhen; Zhu, Weisi; Wang, Zhongqiu; Lin, Jinfeng; Xu, Angao; Wang, Xinying

    2014-04-20

    Methylation-sensitive high-resolution melting (MS-HRM) is a new technique for assaying DNA methylation, but its feasibility for assaying stool in patients with colorectal cancer (CRC) is unknown. First, the MS-HRM and methylation-specific PCR (MSP) detection limits were tested. Second, the methylation statuses of SFRP2 and VIM were analyzed in stool samples by MS-HRM, and in matching tumor and normal colon tissues via bisulfite sequencing PCR (BSP). Third, a case-control study evaluated the diagnostic sensitivity and specificity of MS-HRM relative to results obtained with MSP and the fecal immunochemical test (FIT). Finally, the linearity and reproducibility of MS-HRM were assessed. The detection limits of MS-HRM and MSP were 1% and 5%, respectively. The diagnostic sensitivities of MS-HRM (87.3%, 55/63) in stool and BSP in matching tumor tissue (92.1%, 58/63) were highly consistent (κ=0.744). The MS-HRM assay detected 92.5% (37/40) methylation in CRCs, 94.4% (34/36) in advanced adenomas, and 8.8% (5/57) in normal controls. The results of MS-HRM analysis were stable and reliable and showed fairly good linearity for both SFRP2 (PHRM shows potential for CRC screening. Copyright © 2014 Elsevier B.V. All rights reserved.

  4. Electrochemical noise measurements techniques and the reversing dc potential drop method applied to stress corrosion essays

    International Nuclear Information System (INIS)

    Aly, Omar Fernandes; Andrade, Arnaldo Paes de; MattarNeto, Miguel; Aoki, Idalina Vieira

    2002-01-01

    This paper aims to collect information and to discuss the electrochemical noise measurements and the reversing dc potential drop method, applied to stress corrosion essays that can be used to evaluate the nucleation and the increase of stress corrosion cracking in Alloy 600 and/or Alloy 182 specimens from Angra I Nuclear Power Plant. Therefore we will pretend to establish a standard procedure to essays to be realized on the new autoclave equipment on the Laboratorio de Eletroquimica e Corrosao do Departamento de Engenharia Quimica da Escola Politecnica da Universidade de Sao Paulo - Electrochemical and Corrosion Laboratory of the Chemical Engineering Department of Polytechnical School of Sao Paulo University, Brazil. (author)

  5. Making Design Decisions Visible: Applying the Case-Based Method in Designing Online Instruction

    Directory of Open Access Journals (Sweden)

    Heng Luo,

    2011-01-01

    Full Text Available The instructional intervention in this design case is a self-directed online tutorial that applies the case-based method to teach educators how to design and conduct entrepreneurship programs for elementary school students. In this article, the authors describe the major decisions made in each phase of the design and development process, explicate the rationales behind them, and demonstrate their effect on the production of the tutorial. Based on such analysis, the guidelines for designing case-based online instruction are summarized for the design case.

  6. A semiempirical method of applying the dechanneling correction in the extraction of disorder distribution

    International Nuclear Information System (INIS)

    Walker, R.S.; Thompson, D.A.; Poehlman, S.W.

    1977-01-01

    The application of single, plural or multiple scattering theories to the determination of defect dechanneling in channeling-backscattering disorder measurements is re-examined. A semiempirical modification to the method is described that results in making the extracted disorder and disorder distribution relatively insensitive to the scattering model employed. The various models and modifications have been applied to the 1 to 2 MeV He + channeling-backscatter data obtained from 20 to 80 keV H + to Ne + bombarded Si, GaP and GaAs at 50 K and 300 K. (author)

  7. Zoltàn Dörnyei, Research Methods in Applied Linguistics

    OpenAIRE

    Marie-Françoise Narcy-Combes

    2012-01-01

    Research Methods in Applied Linguistics est un ouvrage pratique et accessible qui s’adresse en priorité au chercheur débutant et au doctorant en linguistique appliquée et en didactique des langues pour lesquels il représente un accompagnement fort utile. Son style clair et son organisation sans surprise en font une lecture facile et agréable et rendent les différents concepts aisément compréhensibles pour tous. Il présente un bilan de la méthodologie de la recherche en linguistique appliquée,...

  8. Cork-resin ablative insulation for complex surfaces and method for applying the same

    Science.gov (United States)

    Walker, H. M.; Sharpe, M. H.; Simpson, W. G. (Inventor)

    1980-01-01

    A method of applying cork-resin ablative insulation material to complex curved surfaces is disclosed. The material is prepared by mixing finely divided cork with a B-stage curable thermosetting resin, forming the resulting mixture into a block, B-stage curing the resin-containing block, and slicing the block into sheets. The B-stage cured sheet is shaped to conform to the surface being insulated, and further curing is then performed. Curing of the resins only to B-stage before shaping enables application of sheet material to complex curved surfaces and avoids limitations and disadvantages presented in handling of fully cured sheet material.

  9. Perturbative methods applied for sensitive coefficients calculations in thermal-hydraulic systems

    International Nuclear Information System (INIS)

    Andrade Lima, F.R. de

    1993-01-01

    The differential formalism and the Generalized Perturbation Theory (GPT) are applied to sensitivity analysis of thermal-hydraulics problems related to pressurized water reactor cores. The equations describing the thermal-hydraulic behavior of these reactors cores, used in COBRA-IV-I code, are conveniently written. The importance function related to the response of interest and the sensitivity coefficient of this response with respect to various selected parameters are obtained by using Differential and Generalized Perturbation Theory. The comparison among the results obtained with the application of these perturbative methods and those obtained directly with the model developed in COBRA-IV-I code shows a very good agreement. (author)

  10. Geometric methods for estimating representative sidewalk widths applied to Vienna's streetscape surfaces database

    Science.gov (United States)

    Brezina, Tadej; Graser, Anita; Leth, Ulrich

    2017-04-01

    Space, and in particular public space for movement and leisure, is a valuable and scarce resource, especially in today's growing urban centres. The distribution and absolute amount of urban space—especially the provision of sufficient pedestrian areas, such as sidewalks—is considered crucial for shaping living and mobility options as well as transport choices. Ubiquitous urban data collection and today's IT capabilities offer new possibilities for providing a relation-preserving overview and for keeping track of infrastructure changes. This paper presents three novel methods for estimating representative sidewalk widths and applies them to the official Viennese streetscape surface database. The first two methods use individual pedestrian area polygons and their geometrical representations of minimum circumscribing and maximum inscribing circles to derive a representative width of these individual surfaces. The third method utilizes aggregated pedestrian areas within the buffered street axis and results in a representative width for the corresponding road axis segment. Results are displayed as city-wide means in a 500 by 500 m grid and spatial autocorrelation based on Moran's I is studied. We also compare the results between methods as well as to previous research, existing databases and guideline requirements on sidewalk widths. Finally, we discuss possible applications of these methods for monitoring and regression analysis and suggest future methodological improvements for increased accuracy.

  11. MAIA - Method for Architecture of Information Applied: methodological construct of information processing in complex contexts

    Directory of Open Access Journals (Sweden)

    Ismael de Moura Costa

    2017-04-01

    Full Text Available Introduction: Paper to presentation the MAIA Method for Architecture of Information Applied evolution, its structure, results obtained and three practical applications.Objective: Proposal of a methodological constructo for treatment of complex information, distinguishing information spaces and revealing inherent configurations of those spaces. Metodology: The argument is elaborated from theoretical research of analitical hallmark, using distinction as a way to express concepts. Phenomenology is used as a philosophical position, which considers the correlation between Subject↔Object. The research also considers the notion of interpretation as an integrating element for concepts definition. With these postulates, the steps to transform the information spaces are formulated. Results: This article explores not only how the method is structured to process information in its contexts, starting from a succession of evolutive cicles, divided in moments, which, on their turn, evolve to transformation acts. Conclusions: This article explores not only how the method is structured to process information in its contexts, starting from a succession of evolutive cicles, divided in moments, which, on their turn, evolve to transformation acts. Besides that, the article presents not only possible applications as a cientific method, but also as configuration tool in information spaces, as well as generator of ontologies. At last, but not least, presents a brief summary of the analysis made by researchers who have already evaluated the method considering the three aspects mentioned.

  12. A Review of Auditing Methods Applied to the Content of Controlled Biomedical Terminologies

    Science.gov (United States)

    Zhu, Xinxin; Fan, Jung-Wei; Baorto, David M.; Weng, Chunhua; Cimino, James J.

    2012-01-01

    Although controlled biomedical terminologies have been with us for centuries, it is only in the last couple of decades that close attention has been paid to the quality of these terminologies. The result of this attention has been the development of auditing methods that apply formal methods to assessing whether terminologies are complete and accurate. We have performed an extensive literature review to identify published descriptions of these methods and have created a framework for characterizing them. The framework considers manual, systematic and heuristic methods that use knowledge (within or external to the terminology) to measure quality factors of different aspects of the terminology content (terms, semantic classification, and semantic relationships). The quality factors examined included concept orientation, consistency, non-redundancy, soundness and comprehensive coverage. We reviewed 130 studies that were retrieved based on keyword search on publications in PubMed, and present our assessment of how they fit into our framework. We also identify which terminologies have been audited with the methods and provide examples to illustrate each part of the framework. PMID:19285571

  13. Knowledge-Based Trajectory Error Pattern Method Applied to an Active Force Control Scheme

    Directory of Open Access Journals (Sweden)

    Endra Pitowarno, Musa Mailah, Hishamuddin Jamaluddin

    2012-08-01

    Full Text Available The active force control (AFC method is known as a robust control scheme that dramatically enhances the performance of a robot arm particularly in compensating the disturbance effects. The main task of the AFC method is to estimate the inertia matrix in the feedback loop to provide the correct (motor torque required to cancel out these disturbances. Several intelligent control schemes have already been introduced to enhance the estimation methods of acquiring the inertia matrix such as those using neural network, iterative learning and fuzzy logic. In this paper, we propose an alternative scheme called Knowledge-Based Trajectory Error Pattern Method (KBTEPM to suppress the trajectory track error of the AFC scheme. The knowledge is developed from the trajectory track error characteristic based on the previous experimental results of the crude approximation method. It produces a unique, new and desirable error pattern when a trajectory command is forced. An experimental study was performed using simulation work on the AFC scheme with KBTEPM applied to a two-planar manipulator in which a set of rule-based algorithm is derived. A number of previous AFC schemes are also reviewed as benchmark. The simulation results show that the AFC-KBTEPM scheme successfully reduces the trajectory track error significantly even in the presence of the introduced disturbances.Key Words:  Active force control, estimated inertia matrix, robot arm, trajectory error pattern, knowledge-based.

  14. Complex Method Mixed with PSO Applying to Optimization Design of Bridge Crane Girder

    Directory of Open Access Journals (Sweden)

    He Yan

    2017-01-01

    Full Text Available In engineer design, basic complex method has not enough global search ability for the nonlinear optimization problem, so it mixed with particle swarm optimization (PSO has been presented in the paper,that is the optimal particle evaluated from fitness function of particle swarm displacement complex vertex in order to realize optimal principle of the largest complex central distance.This method is applied to optimization design problems of box girder of bridge crane with constraint conditions.At first a mathematical model of the girder optimization has been set up,in which box girder cross section area of bridge crane is taken as the objective function, and its four sizes parameters as design variables, girder mechanics performance, manufacturing process, border sizes and so on requirements as constraint conditions. Then complex method mixed with PSO is used to solve optimization design problem of cane box girder from constrained optimization studying approach, and its optimal results have achieved the goal of lightweight design and reducing the crane manufacturing cost . The method is reliable, practical and efficient by the practical engineer calculation and comparative analysis with basic complex method.

  15. Efficient alpha particle detection by CR-39 applying 50 Hz-HV electrochemical etching method

    International Nuclear Information System (INIS)

    Sohrabi, M.; Soltani, Z.

    2016-01-01

    Alpha particles can be detected by CR-39 by applying either chemical etching (CE), electrochemical etching (ECE), or combined pre-etching and ECE usually through a multi-step HF-HV ECE process at temperatures much higher than room temperature. By applying pre-etching, characteristics responses of fast-neutron-induced recoil tracks in CR-39 by HF-HV ECE versus KOH normality (N) have shown two high-sensitivity peaks around 5–6 and 15–16 N and a large-diameter peak with a minimum sensitivity around 10–11 N at 25°C. On the other hand, 50 Hz-HV ECE method recently advanced in our laboratory detects alpha particles with high efficiency and broad registration energy range with small ECE tracks in polycarbonate (PC) detectors. By taking advantage of the CR-39 sensitivity to alpha particles, efficacy of 50 Hz-HV ECE method and CR-39 exotic responses under different KOH normalities, detection characteristics of 0.8 MeV alpha particle tracks were studied in 500 μm CR-39 for different fluences, ECE duration and KOH normality. Alpha registration efficiency increased as ECE duration increased to 90 ± 2% after 6–8 h beyond which plateaus are reached. Alpha track density versus fluence is linear up to 10 6  tracks cm −2 . The efficiency and mean track diameter versus alpha fluence up to 10 6  alphas cm −2 decrease as the fluence increases. Background track density and minimum detection limit are linear functions of ECE duration and increase as normality increases. The CR-39 processed for the first time in this study by 50 Hz-HV ECE method proved to provide a simple, efficient and practical alpha detection method at room temperature. - Highlights: • Alpha particles of 0.8 MeV were detected in CR-39 by 50 Hz-HV ECE method. • Efficiency/track diameter was studied vs fluence and time for 3 KOH normality. • Background track density and minimum detection limit vs duration were studied. • A new simple, efficient and low-cost alpha detection method

  16. A METHOD FOR PREPARING A SUBSTRATE BY APPLYING A SAMPLE TO BE ANALYSED

    DEFF Research Database (Denmark)

    2017-01-01

    The invention relates to a method for preparing a substrate (105a) comprising a sample reception area (110) and a sensing area (111). The method comprises the steps of: 1) applying a sample on the sample reception area; 2) rotating the substrate around a predetermined axis; 3) during rotation......, at least part of the liquid travels from the sample reception area to the sensing area due to capillary forces acting between the liquid and the substrate; and 4) removing the wave of particles and liquid formed at one end of the substrate. The sensing area is closer to the predetermined axis than...... the sample reception area. The sample comprises a liquid part and particles suspended therein....

  17. Simplified inelastic analysis methods applied to fast breeder reactor core design

    International Nuclear Information System (INIS)

    Abo-El-Ata, M.M.

    1978-01-01

    The paper starts with a review of some currently available simplified inelastic analysis methods used in elevated temperature design for evaluating plastic and thermal creep strains. The primary purpose of the paper is to investigate how these simplified methods may be applied to fast breeder reactor core design where neutron irradiation effects are significant. One of the problems discussed is irradiation-induced creep and its effect on shakedown, ratcheting, and plastic cycling. Another problem is the development of swelling-induced stress which is an additional loading mechanism and must be taken into account. In this respect an expression for swelling-induced stress in the presence of irradiation creep is derived and a model for simplifying the stress analysis under these conditions is proposed. As an example, the effects of irradiation creep and swelling induced stress on the analysis of a thin walled tube under constant internal pressure and intermittent heat fluxes, simulating a fuel pin, is presented

  18. Analytical Plug-In Method for Kernel Density Estimator Applied to Genetic Neutrality Study

    Science.gov (United States)

    Troudi, Molka; Alimi, Adel M.; Saoudi, Samir

    2008-12-01

    The plug-in method enables optimization of the bandwidth of the kernel density estimator in order to estimate probability density functions (pdfs). Here, a faster procedure than that of the common plug-in method is proposed. The mean integrated square error (MISE) depends directly upon [InlineEquation not available: see fulltext.] which is linked to the second-order derivative of the pdf. As we intend to introduce an analytical approximation of [InlineEquation not available: see fulltext.], the pdf is estimated only once, at the end of iterations. These two kinds of algorithm are tested on different random variables having distributions known for their difficult estimation. Finally, they are applied to genetic data in order to provide a better characterisation in the mean of neutrality of Tunisian Berber populations.

  19. Analytical Plug-In Method for Kernel Density Estimator Applied to Genetic Neutrality Study

    Directory of Open Access Journals (Sweden)

    Samir Saoudi

    2008-07-01

    Full Text Available The plug-in method enables optimization of the bandwidth of the kernel density estimator in order to estimate probability density functions (pdfs. Here, a faster procedure than that of the common plug-in method is proposed. The mean integrated square error (MISE depends directly upon J(f which is linked to the second-order derivative of the pdf. As we intend to introduce an analytical approximation of J(f, the pdf is estimated only once, at the end of iterations. These two kinds of algorithm are tested on different random variables having distributions known for their difficult estimation. Finally, they are applied to genetic data in order to provide a better characterisation in the mean of neutrality of Tunisian Berber populations.

  20. Infrared thermography inspection methods applied to the target elements of W7-X divertor

    Energy Technology Data Exchange (ETDEWEB)

    Missirlian, M. [Association Euratom-CEA, CEA/DSM/DRFC, CEA/Cadarache, F-13108 Saint Paul Lez Durance (France)], E-mail: marc.missirlian@cea.fr; Traxler, H. [PLANSEE SE, Technology Center, A-6600 Reutte (Austria); Boscary, J. [Max-Planck-Institut fuer Plasmaphysik, Euratom Association, Boltzmannstr. 2, D-85748 Garching (Germany); Durocher, A.; Escourbiac, F.; Schlosser, J. [Association Euratom-CEA, CEA/DSM/DRFC, CEA/Cadarache, F-13108 Saint Paul Lez Durance (France); Schedler, B.; Schuler, P. [PLANSEE SE, Technology Center, A-6600 Reutte (Austria)

    2007-10-15

    The non-destructive examination (NDE) method is one of the key issues in developing highly loaded plasma-facing components (PFCs) for a next generation fusion devices such as W7-X and ITER. The most critical step is certainly the fabrication and the examination of the bond between the armour and the heat sink. Two inspection systems based on the infrared thermography methods, namely, the transient thermography (SATIR-CEA) and the pulsed thermography (ARGUS-PLANSEE), are being developed and have been applied to the pre-series of target elements of the W7-X divertor. Results obtained from qualification experiences performed on target elements with artificial calibrated defects allowed to demonstrate the capability of the two techniques and raised the efficiency of inspection to a level which is appropriate for industrial application.

  1. Infrared thermography inspection methods applied to the target elements of W7-X divertor

    International Nuclear Information System (INIS)

    Missirlian, M.; Traxler, H.; Boscary, J.; Durocher, A.; Escourbiac, F.; Schlosser, J.; Schedler, B.; Schuler, P.

    2007-01-01

    The non-destructive examination (NDE) method is one of the key issues in developing highly loaded plasma-facing components (PFCs) for a next generation fusion devices such as W7-X and ITER. The most critical step is certainly the fabrication and the examination of the bond between the armour and the heat sink. Two inspection systems based on the infrared thermography methods, namely, the transient thermography (SATIR-CEA) and the pulsed thermography (ARGUS-PLANSEE), are being developed and have been applied to the pre-series of target elements of the W7-X divertor. Results obtained from qualification experiences performed on target elements with artificial calibrated defects allowed to demonstrate the capability of the two techniques and raised the efficiency of inspection to a level which is appropriate for industrial application

  2. The fundamental parameter method applied to X-ray fluorescence analysis with synchrotron radiation

    Science.gov (United States)

    Pantenburg, F. J.; Beier, T.; Hennrich, F.; Mommsen, H.

    1992-05-01

    Quantitative X-ray fluorescence analysis applying the fundamental parameter method is usually restricted to monochromatic excitation sources. It is shown here, that such analyses can be performed as well with a white synchrotron radiation spectrum. To determine absolute elemental concentration values it is necessary to know the spectral distribution of this spectrum. A newly designed and tested experimental setup, which uses the synchrotron radiation emitted from electrons in a bending magnet of ELSA (electron stretcher accelerator of the university of Bonn) is presented. The determination of the exciting spectrum, described by the given electron beam parameters, is limited due to uncertainties in the vertical electron beam size and divergence. We describe a method which allows us to determine the relative and absolute spectral distributions needed for accurate analysis. First test measurements of different alloys and standards of known composition demonstrate that it is possible to determine exact concentration values in bulk and trace element analysis.

  3. Super-convergence of Discontinuous Galerkin Method Applied to the Navier-Stokes Equations

    Science.gov (United States)

    Atkins, Harold L.

    2009-01-01

    The practical benefits of the hyper-accuracy properties of the discontinuous Galerkin method are examined. In particular, we demonstrate that some flow attributes exhibit super-convergence even in the absence of any post-processing technique. Theoretical analysis suggest that flow features that are dominated by global propagation speeds and decay or growth rates should be super-convergent. Several discrete forms of the discontinuous Galerkin method are applied to the simulation of unsteady viscous flow over a two-dimensional cylinder. Convergence of the period of the naturally occurring oscillation is examined and shown to converge at 2p+1, where p is the polynomial degree of the discontinuous Galerkin basis. Comparisons are made between the different discretizations and with theoretical analysis.

  4. Data Analytics of Mobile Serious Games: Applying Bayesian Data Analysis Methods

    Directory of Open Access Journals (Sweden)

    Heide Lukosch

    2018-03-01

    Full Text Available Traditional teaching methods in the field of resuscitation training show some limitations, while teaching the right actions in critical situations could increase the number of people saved after a cardiac arrest. For our study, we developed a mobile game to support the transfer of theoretical knowledge on resuscitation.  The game has been tested at three schools of further education. A number of data has been collected from 171 players. To analyze this large data set from different sources and quality, different types of data modeling and analyses had to be applied. This approach showed its usefulness in analyzing the large set of data from different sources. It revealed some interesting findings, such as that female players outperformed the male ones, and that the game fostering informal, self-directed is equally efficient as the traditional formal learning method.

  5. An input feature selection method applied to fuzzy neural networks for signal esitmation

    International Nuclear Information System (INIS)

    Na, Man Gyun; Sim, Young Rok

    2001-01-01

    It is well known that the performance of a fuzzy neural networks strongly depends on the input features selected for its training. In its applications to sensor signal estimation, there are a large number of input variables related with an output. As the number of input variables increases, the training time of fuzzy neural networks required increases exponentially. Thus, it is essential to reduce the number of inputs to a fuzzy neural networks and to select the optimum number of mutually independent inputs that are able to clearly define the input-output mapping. In this work, principal component analysis (PAC), genetic algorithms (GA) and probability theory are combined to select new important input features. A proposed feature selection method is applied to the signal estimation of the steam generator water level, the hot-leg flowrate, the pressurizer water level and the pressurizer pressure sensors in pressurized water reactors and compared with other input feature selection methods

  6. Performance comparison of two efficient genomic selection methods (gsbay & MixP) applied in aquacultural organisms

    Science.gov (United States)

    Su, Hailin; Li, Hengde; Wang, Shi; Wang, Yangfan; Bao, Zhenmin

    2017-02-01

    Genomic selection is more and more popular in animal and plant breeding industries all around the world, as it can be applied early in life without impacting selection candidates. The objective of this study was to bring the advantages of genomic selection to scallop breeding. Two different genomic selection tools MixP and gsbay were applied on genomic evaluation of simulated data and Zhikong scallop ( Chlamys farreri) field data. The data were compared with genomic best linear unbiased prediction (GBLUP) method which has been applied widely. Our results showed that both MixP and gsbay could accurately estimate single-nucleotide polymorphism (SNP) marker effects, and thereby could be applied for the analysis of genomic estimated breeding values (GEBV). In simulated data from different scenarios, the accuracy of GEBV acquired was ranged from 0.20 to 0.78 by MixP; it was ranged from 0.21 to 0.67 by gsbay; and it was ranged from 0.21 to 0.61 by GBLUP. Estimations made by MixP and gsbay were expected to be more reliable than those estimated by GBLUP. Predictions made by gsbay were more robust, while with MixP the computation is much faster, especially in dealing with large-scale data. These results suggested that both algorithms implemented by MixP and gsbay are feasible to carry out genomic selection in scallop breeding, and more genotype data will be necessary to produce genomic estimated breeding values with a higher accuracy for the industry.

  7. Estimating the Impacts of Local Policy Innovation: The Synthetic Control Method Applied to Tropical Deforestation.

    Science.gov (United States)

    Sills, Erin O; Herrera, Diego; Kirkpatrick, A Justin; Brandão, Amintas; Dickson, Rebecca; Hall, Simon; Pattanayak, Subhrendu; Shoch, David; Vedoveto, Mariana; Young, Luisa; Pfaff, Alexander

    2015-01-01

    Quasi-experimental methods increasingly are used to evaluate the impacts of conservation interventions by generating credible estimates of counterfactual baselines. These methods generally require large samples for statistical comparisons, presenting a challenge for evaluating innovative policies implemented within a few pioneering jurisdictions. Single jurisdictions often are studied using comparative methods, which rely on analysts' selection of best case comparisons. The synthetic control method (SCM) offers one systematic and transparent way to select cases for comparison, from a sizeable pool, by focusing upon similarity in outcomes before the intervention. We explain SCM, then apply it to one local initiative to limit deforestation in the Brazilian Amazon. The municipality of Paragominas launched a multi-pronged local initiative in 2008 to maintain low deforestation while restoring economic production. This was a response to having been placed, due to high deforestation, on a federal "blacklist" that increased enforcement of forest regulations and restricted access to credit and output markets. The local initiative included mapping and monitoring of rural land plus promotion of economic alternatives compatible with low deforestation. The key motivation for the program may have been to reduce the costs of blacklisting. However its stated purpose was to limit deforestation, and thus we apply SCM to estimate what deforestation would have been in a (counterfactual) scenario of no local initiative. We obtain a plausible estimate, in that deforestation patterns before the intervention were similar in Paragominas and the synthetic control, which suggests that after several years, the initiative did lower deforestation (significantly below the synthetic control in 2012). This demonstrates that SCM can yield helpful land-use counterfactuals for single units, with opportunities to integrate local and expert knowledge and to test innovations and permutations on policies

  8. Estimating the Impacts of Local Policy Innovation: The Synthetic Control Method Applied to Tropical Deforestation

    Science.gov (United States)

    Sills, Erin O.; Herrera, Diego; Kirkpatrick, A. Justin; Brandão, Amintas; Dickson, Rebecca; Hall, Simon; Pattanayak, Subhrendu; Shoch, David; Vedoveto, Mariana; Young, Luisa; Pfaff, Alexander

    2015-01-01

    Quasi-experimental methods increasingly are used to evaluate the impacts of conservation interventions by generating credible estimates of counterfactual baselines. These methods generally require large samples for statistical comparisons, presenting a challenge for evaluating innovative policies implemented within a few pioneering jurisdictions. Single jurisdictions often are studied using comparative methods, which rely on analysts’ selection of best case comparisons. The synthetic control method (SCM) offers one systematic and transparent way to select cases for comparison, from a sizeable pool, by focusing upon similarity in outcomes before the intervention. We explain SCM, then apply it to one local initiative to limit deforestation in the Brazilian Amazon. The municipality of Paragominas launched a multi-pronged local initiative in 2008 to maintain low deforestation while restoring economic production. This was a response to having been placed, due to high deforestation, on a federal “blacklist” that increased enforcement of forest regulations and restricted access to credit and output markets. The local initiative included mapping and monitoring of rural land plus promotion of economic alternatives compatible with low deforestation. The key motivation for the program may have been to reduce the costs of blacklisting. However its stated purpose was to limit deforestation, and thus we apply SCM to estimate what deforestation would have been in a (counterfactual) scenario of no local initiative. We obtain a plausible estimate, in that deforestation patterns before the intervention were similar in Paragominas and the synthetic control, which suggests that after several years, the initiative did lower deforestation (significantly below the synthetic control in 2012). This demonstrates that SCM can yield helpful land-use counterfactuals for single units, with opportunities to integrate local and expert knowledge and to test innovations and permutations on

  9. Estimating the Impacts of Local Policy Innovation: The Synthetic Control Method Applied to Tropical Deforestation.

    Directory of Open Access Journals (Sweden)

    Erin O Sills

    Full Text Available Quasi-experimental methods increasingly are used to evaluate the impacts of conservation interventions by generating credible estimates of counterfactual baselines. These methods generally require large samples for statistical comparisons, presenting a challenge for evaluating innovative policies implemented within a few pioneering jurisdictions. Single jurisdictions often are studied using comparative methods, which rely on analysts' selection of best case comparisons. The synthetic control method (SCM offers one systematic and transparent way to select cases for comparison, from a sizeable pool, by focusing upon similarity in outcomes before the intervention. We explain SCM, then apply it to one local initiative to limit deforestation in the Brazilian Amazon. The municipality of Paragominas launched a multi-pronged local initiative in 2008 to maintain low deforestation while restoring economic production. This was a response to having been placed, due to high deforestation, on a federal "blacklist" that increased enforcement of forest regulations and restricted access to credit and output markets. The local initiative included mapping and monitoring of rural land plus promotion of economic alternatives compatible with low deforestation. The key motivation for the program may have been to reduce the costs of blacklisting. However its stated purpose was to limit deforestation, and thus we apply SCM to estimate what deforestation would have been in a (counterfactual scenario of no local initiative. We obtain a plausible estimate, in that deforestation patterns before the intervention were similar in Paragominas and the synthetic control, which suggests that after several years, the initiative did lower deforestation (significantly below the synthetic control in 2012. This demonstrates that SCM can yield helpful land-use counterfactuals for single units, with opportunities to integrate local and expert knowledge and to test innovations and

  10. Applying Mathematical Optimization Methods to an ACT-R Instance-Based Learning Model.

    Directory of Open Access Journals (Sweden)

    Nadia Said

    Full Text Available Computational models of cognition provide an interface to connect advanced mathematical tools and methods to empirically supported theories of behavior in psychology, cognitive science, and neuroscience. In this article, we consider a computational model of instance-based learning, implemented in the ACT-R cognitive architecture. We propose an approach for obtaining mathematical reformulations of such cognitive models that improve their computational tractability. For the well-established Sugar Factory dynamic decision making task, we conduct a simulation study to analyze central model parameters. We show how mathematical optimization techniques can be applied to efficiently identify optimal parameter values with respect to different optimization goals. Beyond these methodological contributions, our analysis reveals the sensitivity of this particular task with respect to initial settings and yields new insights into how average human performance deviates from potential optimal performance. We conclude by discussing possible extensions of our approach as well as future steps towards applying more powerful derivative-based optimization methods.

  11. Labile soil phosphorus as influenced by methods of applying radioactive phosphorus

    International Nuclear Information System (INIS)

    Selvaratnam, V.V.; Andersen, A.J.; Thomsen, J.D.; Gissel-Nielsen, G.

    1980-03-01

    The influence of different methods of applying radioactive phosphorus on the E- and L-values was studied in four foil types using barley, buckwheat, and rye grass for the L-value determination. The four soils differed greatly in their E- and L-values. The experiment was carried out both with and without carrier-P. The presence of carrier-P had no influence on the E-values, while carrier-P in some cases gave a lower L-value. Both E- and L-values dependent on the method of application. When the 32 P was applied on a small soil or sand sample and dried before mixing with the total amount of soil, the E-values were higher than at direct application most likely because of a stronger fixation to the soil/sand particles. This was not the case for the L-values that are based on a much longer equilibrium time. On the contrary, the direct application of the 32 p-solution to the whole amount of soil gave higher L-values of a non-homogeneous distribution of the 32 p in the soil. (author)

  12. Analysis of coupled neutron-gamma radiations, applied to shieldings in multigroup albedo method

    International Nuclear Information System (INIS)

    Dunley, Leonardo Souza

    2002-01-01

    The principal mathematical tools frequently available for calculations in Nuclear Engineering, including coupled neutron-gamma radiations shielding problems, involve the full Transport Theory or the Monte Carlo techniques. The Multigroup Albedo Method applied to shieldings is characterized by following the radiations through distinct layers of materials, allowing the determination of the neutron and gamma fractions reflected from, transmitted through and absorbed in the irradiated media when a neutronic stream hits the first layer of material, independently of flux calculations. Then, the method is a complementary tool of great didactic value due to its clarity and simplicity in solving neutron and/or gamma shielding problems. The outstanding results achieved in previous works motivated the elaboration and the development of this study that is presented in this dissertation. The radiation balance resulting from the incidence of a neutronic stream into a shielding composed by 'm' non-multiplying slab layers for neutrons was determined by the Albedo method, considering 'n' energy groups for neutrons and 'g' energy groups for gammas. It was taken into account there is no upscattering of neutrons and gammas. However, it was considered that neutrons from any energy groups are able to produce gammas of all energy groups. The ANISN code, for an angular quadrature order S 2 , was used as a standard for comparison of the results obtained by the Albedo method. So, it was necessary to choose an identical system configuration, both for ANISN and Albedo methods. This configuration was six neutron energy groups and eight gamma energy groups, using three slab layers (iron aluminum - manganese). The excellent results expressed in comparative tables show great agreement between the values determined by the deterministic code adopted as standard and, the values determined by the computational program created using the Albedo method and the algorithm developed for coupled neutron

  13. In silico toxicology: comprehensive benchmarking of multi-label classification methods applied to chemical toxicity data

    KAUST Repository

    Raies, Arwa B.

    2017-12-05

    One goal of toxicity testing, among others, is identifying harmful effects of chemicals. Given the high demand for toxicity tests, it is necessary to conduct these tests for multiple toxicity endpoints for the same compound. Current computational toxicology methods aim at developing models mainly to predict a single toxicity endpoint. When chemicals cause several toxicity effects, one model is generated to predict toxicity for each endpoint, which can be labor and computationally intensive when the number of toxicity endpoints is large. Additionally, this approach does not take into consideration possible correlation between the endpoints. Therefore, there has been a recent shift in computational toxicity studies toward generating predictive models able to predict several toxicity endpoints by utilizing correlations between these endpoints. Applying such correlations jointly with compounds\\' features may improve model\\'s performance and reduce the number of required models. This can be achieved through multi-label classification methods. These methods have not undergone comprehensive benchmarking in the domain of predictive toxicology. Therefore, we performed extensive benchmarking and analysis of over 19,000 multi-label classification models generated using combinations of the state-of-the-art methods. The methods have been evaluated from different perspectives using various metrics to assess their effectiveness. We were able to illustrate variability in the performance of the methods under several conditions. This review will help researchers to select the most suitable method for the problem at hand and provide a baseline for evaluating new approaches. Based on this analysis, we provided recommendations for potential future directions in this area.

  14. In silico toxicology: comprehensive benchmarking of multi-label classification methods applied to chemical toxicity data

    KAUST Repository

    Raies, Arwa B.; Bajic, Vladimir B.

    2017-01-01

    One goal of toxicity testing, among others, is identifying harmful effects of chemicals. Given the high demand for toxicity tests, it is necessary to conduct these tests for multiple toxicity endpoints for the same compound. Current computational toxicology methods aim at developing models mainly to predict a single toxicity endpoint. When chemicals cause several toxicity effects, one model is generated to predict toxicity for each endpoint, which can be labor and computationally intensive when the number of toxicity endpoints is large. Additionally, this approach does not take into consideration possible correlation between the endpoints. Therefore, there has been a recent shift in computational toxicity studies toward generating predictive models able to predict several toxicity endpoints by utilizing correlations between these endpoints. Applying such correlations jointly with compounds' features may improve model's performance and reduce the number of required models. This can be achieved through multi-label classification methods. These methods have not undergone comprehensive benchmarking in the domain of predictive toxicology. Therefore, we performed extensive benchmarking and analysis of over 19,000 multi-label classification models generated using combinations of the state-of-the-art methods. The methods have been evaluated from different perspectives using various metrics to assess their effectiveness. We were able to illustrate variability in the performance of the methods under several conditions. This review will help researchers to select the most suitable method for the problem at hand and provide a baseline for evaluating new approaches. Based on this analysis, we provided recommendations for potential future directions in this area.

  15. Study on Feasibility of Applying Function Approximation Moment Method to Achieve Reliability-Based Design Optimization

    International Nuclear Information System (INIS)

    Huh, Jae Sung; Kwak, Byung Man

    2011-01-01

    Robust optimization or reliability-based design optimization are some of the methodologies that are employed to take into account the uncertainties of a system at the design stage. For applying such methodologies to solve industrial problems, accurate and efficient methods for estimating statistical moments and failure probability are required, and further, the results of sensitivity analysis, which is needed for searching direction during the optimization process, should also be accurate. The aim of this study is to employ the function approximation moment method into the sensitivity analysis formulation, which is expressed as an integral form, to verify the accuracy of the sensitivity results, and to solve a typical problem of reliability-based design optimization. These results are compared with those of other moment methods, and the feasibility of the function approximation moment method is verified. The sensitivity analysis formula with integral form is the efficient formulation for evaluating sensitivity because any additional function calculation is not needed provided the failure probability or statistical moments are calculated

  16. LOGICAL CONDITIONS ANALYSIS METHOD FOR DIAGNOSTIC TEST RESULTS DECODING APPLIED TO COMPETENCE ELEMENTS PROFICIENCY

    Directory of Open Access Journals (Sweden)

    V. I. Freyman

    2015-11-01

    Full Text Available Subject of Research.Representation features of education results for competence-based educational programs are analyzed. Solution importance of decoding and proficiency estimation for elements and components of discipline parts of competences is shown. The purpose and objectives of research are formulated. Methods. The paper deals with methods of mathematical logic, Boolean algebra, and parametrical analysis of complex diagnostic test results, that controls proficiency of some discipline competence elements. Results. The method of logical conditions analysis is created. It will give the possibility to formulate logical conditions for proficiency determination of each discipline competence element, controlled by complex diagnostic test. Normalized test result is divided into noncrossing zones; a logical condition about controlled elements proficiency is formulated for each of them. Summarized characteristics for test result zones are imposed. An example of logical conditions forming for diagnostic test with preset features is provided. Practical Relevance. The proposed method of logical conditions analysis is applied in the decoding algorithm of proficiency test diagnosis for discipline competence elements. It will give the possibility to automate the search procedure for elements with insufficient proficiency, and is also usable for estimation of education results of a discipline or a component of competence-based educational program.

  17. An IMU-to-Body Alignment Method Applied to Human Gait Analysis

    Directory of Open Access Journals (Sweden)

    Laura Susana Vargas-Valencia

    2016-12-01

    Full Text Available This paper presents a novel calibration procedure as a simple, yet powerful, method to place and align inertial sensors with body segments. The calibration can be easily replicated without the need of any additional tools. The proposed method is validated in three different applications: a computer mathematical simulation; a simplified joint composed of two semi-spheres interconnected by a universal goniometer; and a real gait test with five able-bodied subjects. Simulation results demonstrate that, after the calibration method is applied, the joint angles are correctly measured independently of previous sensor placement on the joint, thus validating the proposed procedure. In the cases of a simplified joint and a real gait test with human volunteers, the method also performs correctly, although secondary plane errors appear when compared with the simulation results. We believe that such errors are caused by limitations of the current inertial measurement unit (IMU technology and fusion algorithms. In conclusion, the presented calibration procedure is an interesting option to solve the alignment problem when using IMUs for gait analysis.

  18. The Cn method applied to problems with an anisotropic diffusion law

    International Nuclear Information System (INIS)

    Grandjean, P.M.

    A 2-dimensional Cn calculation has been applied to homogeneous media subjected to the Rayleigh impact law. Results obtained with collision probabilities and Chandrasekhar calculations are compared to those from Cn method. Introducing in the expression of the transport equation, an expansion truncated on a polynomial basis for the outgoing angular flux (or possibly entrance flux) gives two Cn systems of algebraic linear equations for the expansion coefficients. The matrix elements of these equations are the moments of the Green function in infinite medium. The search for the Green function is effected through the Fourier transformation of the integrodifferential equation and its moments are derived from their Fourier transforms through a numerical integration in the complex plane. The method has been used for calculating the albedo in semi-infinite media, the extrapolation length of the Milne problem, and the albedo and transmission factor of a slab (a concise study of convergence is presented). A system of integro-differential equations bearing on the moments of the angular flux inside the medium has been derived, for the collision probability method. It is numerically solved with approximately the bulk flux by step functions. The albedo in semi-infinite medium has also been computed through the semi-analytical Chandrasekhar method. In the latter, the outgoing flux is expressed as a function of the entrance flux by means of a integral whose kernel is numerically derived [fr

  19. A statistical method for testing epidemiological results, as applied to the Hanford worker population

    International Nuclear Information System (INIS)

    Brodsky, A.

    1979-01-01

    Some recent reports of Mancuso, Stewart and Kneale claim findings of radiation-produced cancer in the Hanford worker population. These claims are based on statistical computations that use small differences in accumulated exposures between groups dying of cancer and groups dying of other causes; actual mortality and longevity were not reported. This paper presents a statistical method for evaluation of actual mortality and longevity longitudinally over time, as applied in a primary analysis of the mortality experience of the Hanford worker population. Although available, this method was not utilized in the Mancuso-Stewart-Kneale paper. The author's preliminary longitudinal analysis shows that the gross mortality experience of persons employed at Hanford during 1943-70 interval did not differ significantly from that of certain controls, when both employees and controls were selected from families with two or more offspring and comparison were matched by age, sex, race and year of entry into employment. This result is consistent with findings reported by Sanders (Health Phys. vol.35, 521-538, 1978). The method utilizes an approximate chi-square (1 D.F.) statistic for testing population subgroup comparisons, as well as the cumulation of chi-squares (1 D.F.) for testing the overall result of a particular type of comparison. The method is available for computer testing of the Hanford mortality data, and could also be adapted to morbidity or other population studies. (author)

  20. Applying Novel Time-Frequency Moments Singular Value Decomposition Method and Artificial Neural Networks for Ballistocardiography

    Directory of Open Access Journals (Sweden)

    Koivistoinen Teemu

    2007-01-01

    Full Text Available As we know, singular value decomposition (SVD is designed for computing singular values (SVs of a matrix. Then, if it is used for finding SVs of an -by-1 or 1-by- array with elements representing samples of a signal, it will return only one singular value that is not enough to express the whole signal. To overcome this problem, we designed a new kind of the feature extraction method which we call ''time-frequency moments singular value decomposition (TFM-SVD.'' In this new method, we use statistical features of time series as well as frequency series (Fourier transform of the signal. This information is then extracted into a certain matrix with a fixed structure and the SVs of that matrix are sought. This transform can be used as a preprocessing stage in pattern clustering methods. The results in using it indicate that the performance of a combined system including this transform and classifiers is comparable with the performance of using other feature extraction methods such as wavelet transforms. To evaluate TFM-SVD, we applied this new method and artificial neural networks (ANNs for ballistocardiogram (BCG data clustering to look for probable heart disease of six test subjects. BCG from the test subjects was recorded using a chair-like ballistocardiograph, developed in our project. This kind of device combined with automated recording and analysis would be suitable for use in many places, such as home, office, and so forth. The results show that the method has high performance and it is almost insensitive to BCG waveform latency or nonlinear disturbance.