WorldWideScience

Sample records for holographic microscopy based

  1. Sparsity-based multi-height phase recovery in holographic microscopy

    Science.gov (United States)

    Rivenson, Yair; Wu, Yichen; Wang, Hongda; Zhang, Yibo; Feizi, Alborz; Ozcan, Aydogan

    2016-11-01

    High-resolution imaging of densely connected samples such as pathology slides using digital in-line holographic microscopy requires the acquisition of several holograms, e.g., at >6-8 different sample-to-sensor distances, to achieve robust phase recovery and coherent imaging of specimen. Reducing the number of these holographic measurements would normally result in reconstruction artifacts and loss of image quality, which would be detrimental especially for biomedical and diagnostics-related applications. Inspired by the fact that most natural images are sparse in some domain, here we introduce a sparsity-based phase reconstruction technique implemented in wavelet domain to achieve at least 2-fold reduction in the number of holographic measurements for coherent imaging of densely connected samples with minimal impact on the reconstructed image quality, quantified using a structural similarity index. We demonstrated the success of this approach by imaging Papanicolaou smears and breast cancer tissue slides over a large field-of-view of ~20 mm2 using 2 in-line holograms that are acquired at different sample-to-sensor distances and processed using sparsity-based multi-height phase recovery. This new phase recovery approach that makes use of sparsity can also be extended to other coherent imaging schemes, involving e.g., multiple illumination angles or wavelengths to increase the throughput and speed of coherent imaging.

  2. Sparsity-based multi-height phase recovery in holographic microscopy

    KAUST Repository

    Rivenson, Yair

    2016-11-30

    High-resolution imaging of densely connected samples such as pathology slides using digital in-line holographic microscopy requires the acquisition of several holograms, e.g., at >6–8 different sample-to-sensor distances, to achieve robust phase recovery and coherent imaging of specimen. Reducing the number of these holographic measurements would normally result in reconstruction artifacts and loss of image quality, which would be detrimental especially for biomedical and diagnostics-related applications. Inspired by the fact that most natural images are sparse in some domain, here we introduce a sparsity-based phase reconstruction technique implemented in wavelet domain to achieve at least 2-fold reduction in the number of holographic measurements for coherent imaging of densely connected samples with minimal impact on the reconstructed image quality, quantified using a structural similarity index. We demonstrated the success of this approach by imaging Papanicolaou smears and breast cancer tissue slides over a large field-of-view of ~20 mm2 using 2 in-line holograms that are acquired at different sample-to-sensor distances and processed using sparsity-based multi-height phase recovery. This new phase recovery approach that makes use of sparsity can also be extended to other coherent imaging schemes, involving e.g., multiple illumination angles or wavelengths to increase the throughput and speed of coherent imaging.

  3. Automatic phase aberration compensation for digital holographic microscopy based on deep learning background detection.

    Science.gov (United States)

    Nguyen, Thanh; Bui, Vy; Lam, Van; Raub, Christopher B; Chang, Lin-Ching; Nehmetallah, George

    2017-06-26

    We propose a fully automatic technique to obtain aberration free quantitative phase imaging in digital holographic microscopy (DHM) based on deep learning. The traditional DHM solves the phase aberration compensation problem by manually detecting the background for quantitative measurement. This would be a drawback in real time implementation and for dynamic processes such as cell migration phenomena. A recent automatic aberration compensation approach using principle component analysis (PCA) in DHM avoids human intervention regardless of the cells' motion. However, it corrects spherical/elliptical aberration only and disregards the higher order aberrations. Traditional image segmentation techniques can be employed to spatially detect cell locations. Ideally, automatic image segmentation techniques make real time measurement possible. However, existing automatic unsupervised segmentation techniques have poor performance when applied to DHM phase images because of aberrations and speckle noise. In this paper, we propose a novel method that combines a supervised deep learning technique with convolutional neural network (CNN) and Zernike polynomial fitting (ZPF). The deep learning CNN is implemented to perform automatic background region detection that allows for ZPF to compute the self-conjugated phase to compensate for most aberrations.

  4. Holographic fluorescence microscopy with incoherent digital holographic adaptive optics.

    Science.gov (United States)

    Jang, Changwon; Kim, Jonghyun; Clark, David C; Lee, Seungjae; Lee, Byoungho; Kim, Myung K

    2015-01-01

    Introduction of adaptive optics technology into astronomy and ophthalmology has made great contributions in these fields, allowing one to recover images blurred by atmospheric turbulence or aberrations of the eye. Similar adaptive optics improvement in microscopic imaging is also of interest to researchers using various techniques. Current technology of adaptive optics typically contains three key elements: a wavefront sensor, wavefront corrector, and controller. These hardware elements tend to be bulky, expensive, and limited in resolution, involving, for example, lenslet arrays for sensing or multiactuator deformable mirrors for correcting. We have previously introduced an alternate approach based on unique capabilities of digital holography, namely direct access to the phase profile of an optical field and the ability to numerically manipulate the phase profile. We have also demonstrated that direct access and compensation of the phase profile are possible not only with conventional coherent digital holography, but also with a new type of digital holography using incoherent light: selfinterference incoherent digital holography (SIDH). The SIDH generates a complex—i.e., amplitude plus phase—hologram from one or several interferograms acquired with incoherent light, such as LEDs, lamps, sunlight, or fluorescence. The complex point spread function can be measured using guide star illumination and it allows deterministic deconvolution of the full-field image. We present experimental demonstration of aberration compensation in holographic fluorescence microscopy using SIDH. Adaptive optics by SIDH provides new tools for improved cellular fluorescence microscopy through intact tissue layers or other types of aberrant media.

  5. High efficiency and flexible working distance digital in-line holographic microscopy based on Fresnel zone plate

    International Nuclear Information System (INIS)

    Tian, Peng; Yang, Fan; Li, Fanxing; Hu, Song; Yan, Wei; Hua, Yilei

    2017-01-01

    Traditional digital in-line holography suffers from twin-image noise problems and extremely short working distances between the object and light source. Here, we propose lensless Fourier transform digital in-line holographic microscopy based on a single optical element. A Fresnel zone plate is used to split the incident light into two parts: one is scattered along the original direction, the other is gathered at a focal point and the sample is put behind the focus. The interference fringe pattern, formed by the two beams, is recorded digitally by a CCD camera. A novel reconstruction algorithm is proposed to present the object image. The proof-of-concept experiments demonstrate that the proposed technique can eliminate the twin-image noise problem, improving the image contrast with high efficiency, and increasing the flexibility of the working distance. Furthermore, a wide field of view and no contact make it a promising tool for the study of materials science, biology and microelectronics. (paper)

  6. Digital Holographic Microscopy Principles, Techniques, and Applications

    CERN Document Server

    Kim, Myung K

    2011-01-01

    Digital holography is an emerging field of new paradigm in general imaging applications. By replacing the photochemical procedures with electronic imaging and having a direct numerical access to the complex optical field, a wide range of new imaging capabilities become available, many of them difficult or infeasible in conventional holography. An increasing number of researchers—not only in optical physics and optical engineering, but also in diverse applications areas such as microbiology, medicine, marine science, particle analysis, microelectromechanics, and metrology—are realizing and exploiting the new capabilities of digital holography. Digital Holographic Microscopy: Principles, Techniques, and Applications, by Dr. Myung K. Kim, is intended to provide a brief but consistent introduction to the principles of digital holography as well as to give an organized overview of the large number of techniques and applications being developed. This will also shed some light on the range of possibilities for f...

  7. Phase-shifting Real-time Holographic Microscopy applied in micro-structures surface analysis

    International Nuclear Information System (INIS)

    Brito, I V; Gesualdi, M R R; Muramatsu, M; Ricardo, J

    2011-01-01

    The microscopic real-time analysis of micro structured materials is of great importance in various domains of science and technology. For other hand, the holographic interferometry comprises a group of powerful optical methods for non-destructive testing in surface analysis. The holographic microscopy uses the holographic interferometric techniques to obtain quantitative intensity and phase information of the optical waves by microscopic systems. With the development of CCD cameras, computers (hardware and software), and new materials for holographic recording, these techniques can be used to replace the classical form of registration and became promising tools in surface analysis. In this work, we developed a prototype of Photorefractive and Digital Holographic Microscope for real-time analysis of micro-structured systems based on the phase-shifting real-time holographic interferometry techniques. Using this apparatus, we are made analysis of shapes and surfaces to obtain the phase maps and the 3D profiles of some samples.

  8. Exploring Neural Cell Dynamics with Digital Holographic Microscopy

    KAUST Repository

    Marquet, Pierre; Jourdain, Pascal; Boss, Daniel; Depeursinge, Christian D.; Magistretti, Pierre J.

    2013-01-01

    In this talk, I will present how digital holographic microscopy, as a powerful quantitative phase technique, can non-invasively measure cell dynamics and especially resolve local neuronal network activity through simultaneous multiple site optical recording.

  9. Exploring Neural Cell Dynamics with Digital Holographic Microscopy

    KAUST Repository

    Marquet, Pierre

    2013-04-21

    In this talk, I will present how digital holographic microscopy, as a powerful quantitative phase technique, can non-invasively measure cell dynamics and especially resolve local neuronal network activity through simultaneous multiple site optical recording.

  10. Exploring neural cell dynamics with digital holographic microscopy

    KAUST Repository

    Marquet, Pierre; Depeursinge, Christian D.; Magistretti, Pierre J.

    2013-01-01

    In this review, we summarize how the new concept of digital optics applied to the field of holographic microscopy has allowed the development of a reliable and flexible digital holographic quantitative phase microscopy (DH-QPM) technique at the nanoscale particularly suitable for cell imaging. Particular emphasis is placed on the original biological ormation provided by the quantitative phase signal. We present the most relevant DH-QPM applications in the field of cell biology, including automated cell counts, recognition, classification, three-dimensional tracking, discrimination between physiological and pathophysiological states, and the study of cell membrane fluctuations at the nanoscale. In the last part, original results show how DH-QPM can address two important issues in the field of neurobiology, namely, multiple-site optical recording of neuronal activity and noninvasive visualization of dendritic spine dynamics resulting from a full digital holographic microscopy tomographic approach. Copyright © 2013 by Annual Reviews.

  11. Exploring neural cell dynamics with digital holographic microscopy

    KAUST Repository

    Marquet, Pierre

    2013-07-11

    In this review, we summarize how the new concept of digital optics applied to the field of holographic microscopy has allowed the development of a reliable and flexible digital holographic quantitative phase microscopy (DH-QPM) technique at the nanoscale particularly suitable for cell imaging. Particular emphasis is placed on the original biological ormation provided by the quantitative phase signal. We present the most relevant DH-QPM applications in the field of cell biology, including automated cell counts, recognition, classification, three-dimensional tracking, discrimination between physiological and pathophysiological states, and the study of cell membrane fluctuations at the nanoscale. In the last part, original results show how DH-QPM can address two important issues in the field of neurobiology, namely, multiple-site optical recording of neuronal activity and noninvasive visualization of dendritic spine dynamics resulting from a full digital holographic microscopy tomographic approach. Copyright © 2013 by Annual Reviews.

  12. Coherent imaging with incoherent light in digital holographic microscopy

    Science.gov (United States)

    Chmelik, Radim

    2012-01-01

    Digital holographic microscope (DHM) allows for imaging with a quantitative phase contrast. In this way it becomes an important instrument, a completely non-invasive tool for a contrast intravital observation of living cells and a cell drymass density distribution measurement. A serious drawback of current DHMs is highly coherent illumination which makes the lateral resolution worse and impairs the image quality by a coherence noise and a parasitic interference. An uncompromising solution to this problem can be found in the Leith concept of incoherent holography. An off-axis hologram can be formed with arbitrary degree of light coherence in systems equipped with an achromatic interferometer and thus the resolution and the image quality typical for an incoherent-light wide-field microscopy can be achieved. In addition, advanced imaging modes based on limited coherence can be utilized. The typical example is a coherence-gating effect which provides a finite axial resolution and makes DHM image similar to that of a confocal microscope. These possibilities were described theoretically using the formalism of three-dimensional coherent transfer functions and proved experimentally by the coherence-controlled holographic microscope which is DHM based on the Leith achromatic interferometer. Quantitative-phase-contrast imaging is demonstrated with incoherent light by the living cancer cells observation and their motility evaluation. The coherence-gating effect was proved by imaging of model samples through a scattering layer and living cells inside an opalescent medium.

  13. Efficient Phase Unwrapping Architecture for Digital Holographic Microscopy

    Directory of Open Access Journals (Sweden)

    Wen-Jyi Hwang

    2011-09-01

    Full Text Available This paper presents a novel phase unwrapping architecture for accelerating the computational speed of digital holographic microscopy (DHM. A fast Fourier transform (FFT based phase unwrapping algorithm providing a minimum squared error solution is adopted for hardware implementation because of its simplicity and robustness to noise. The proposed architecture is realized in a pipeline fashion to maximize through put of thecomputation. Moreover, the number of hardware multipliers and dividers are minimized to reduce the hardware costs. The proposed architecture is used as a custom user logic in a system on programmable chip (SOPC for physical performance measurement. Experimental results reveal that the proposed architecture is effective for expediting the computational speed while consuming low hardware resources for designing an embedded DHM system.

  14. Digital holographic microscopy of phase separation in multicomponent lipid membranes

    Science.gov (United States)

    Farzam Rad, Vahideh; Moradi, Ali-Reza; Darudi, Ahmad; Tayebi, Lobat

    2016-12-01

    Lateral in-homogeneities in lipid compositions cause microdomains formation and change in the physical properties of biological membranes. With the presence of cholesterol and mixed species of lipids, phospholipid membranes segregate into lateral domains of liquid-ordered and liquid-disordered phases. Coupling of two-dimensional intralayer phase separations and interlayer liquid-crystalline ordering in multicomponent membranes has been previously demonstrated. By the use of digital holographic microscopy (DHMicroscopy), we quantitatively analyzed the volumetric dynamical behavior of such membranes. The specimens are lipid mixtures composed of sphingomyelin, cholesterol, and unsaturated phospholipid, 1,2-dioleoyl-sn-glycero-3-phosphocholine. DHMicroscopy in a transmission mode is an effective tool for quantitative visualization of phase objects. By deriving the associated phase changes, three-dimensional information on the morphology variation of lipid stacks at arbitrary time scales is obtained. Moreover, the thickness distribution of the object at demanded axial planes can be obtained by numerical focusing. Our results show that the volume evolution of lipid domains follows approximately the same universal growth law of previously reported area evolution. However, the thickness of the domains does not alter significantly by time; therefore, the volume evolution is mostly attributed to the changes in area dynamics. These results might be useful in the field of membrane-based functional materials.

  15. Intelligent interaction based on holographic personalized portal

    Directory of Open Access Journals (Sweden)

    Yadong Huang

    2017-06-01

    Full Text Available Purpose – The purpose of this paper is to study the architecture of holographic personalized portal, user modeling, commodity modeling and intelligent interaction. Design/methodology/approach – In this paper, the authors propose crowd-science industrial ecological system based on holographic personalized portal and its interaction. The holographic personality portal is based on holographic enterprises, commodities and consumers, and the personalized portal consists of accurate ontology, reliable supply, intelligent demand and smart cyberspace. Findings – The personalized portal can realize the information acquisition, characteristic analysis and holographic presentation. Then, the intelligent interaction, e.g. demand decomposition, personalized search, personalized presentation and demand prediction, will be implemented within the personalized portal. Originality/value – The authors believe that their work on intelligent interaction based on holographic personalized portal, which has been first proposed in this paper, is innovation focusing on the interaction between intelligence and convenience.

  16. Digital Holographic Microscopy: Quantitative Phase Imaging and Applications in Live Cell Analysis

    Science.gov (United States)

    Kemper, Björn; Langehanenberg, Patrik; Kosmeier, Sebastian; Schlichthaber, Frank; Remmersmann, Christian; von Bally, Gert; Rommel, Christina; Dierker, Christian; Schnekenburger, Jürgen

    The analysis of complex processes in living cells creates a high demand for fast and label-free methods for online monitoring. Widely used fluorescence methods require specific labeling and are often restricted to chemically fixated samples. Thus, methods that offer label-free and minimally invasive detection of live cell processes and cell state alterations are of particular interest. In combination with light microscopy, digital holography provides label-free, multi-focus quantitative phase imaging of living cells. In overview, several methods for digital holographic microscopy (DHM) are presented. First, different experimental setups for the recording of digital holograms and the modular integration of DHM into common microscopes are described. Then the numerical processing of digitally captured holograms is explained. This includes the description of spatial and temporal phase shifting techniques, spatial filtering based reconstruction, holographic autofocusing, and the evaluation of self-interference holograms. Furthermore, the usage of partial coherent light and multi-wavelength approaches is discussed. Finally, potentials of digital holographic microscopy for quantitative cell imaging are illustrated by results from selected applications. It is shown that DHM can be used for automated tracking of migrating cells and cell thickness monitoring as well as for refractive index determination of cells and particles. Moreover, the use of DHM for label-free analysis in fluidics and micro-injection monitoring is demonstrated. The results show that DHM is a highly relevant method that allows novel insights in dynamic cell biology, with applications in cancer research and for drugs and toxicity testing.

  17. Quantitative phase imaging with scanning holographic microscopy: an experimental assesment

    Directory of Open Access Journals (Sweden)

    Tada Yoshitaka

    2006-11-01

    Full Text Available Abstract This paper demonstrates experimentally how quantitative phase information can be obtained in scanning holographic microscopy. Scanning holography can operate in both coherent and incoherent modes, simultaneously if desired, with different detector geometries. A spatially integrating detector provides an incoherent hologram of the object's intensity distribution (absorption and/or fluorescence, for example, while a point detector in a conjugate plane of the pupil provides a coherent hologram of the object's complex amplitude, from which a quantitative measure of its phase distribution can be extracted. The possibility of capturing simultaneously holograms of three-dimensional specimens, leading to three-dimensional reconstructions with absorption contrast, reflectance contrast, fluorescence contrast, as was previously demonstrated, and quantitative phase contrast, as shown here for the first time, opens up new avenues for multimodal imaging in biological studies.

  18. Single beam Fourier transform digital holographic quantitative phase microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Anand, A., E-mail: arun-nair-in@yahoo.com; Chhaniwal, V. K.; Mahajan, S.; Trivedi, V. [Optics Laboratory, Applied Physics Department, Faculty of Technology and Engineering, M.S. University of Baroda, Vadodara 390001 (India); Faridian, A.; Pedrini, G.; Osten, W. [Institut für Technische Optik, Universität Stuttgart, Pfaffenwaldring 9, 70569 Stuttgart (Germany); Dubey, S. K. [Siemens Technology and Services Pvt. Ltd, Corporate Technology—Research and Technology Centre, Bangalore 560100 (India); Javidi, B. [Department of Electrical and Computer Engineering, U-4157, University of Connecticut, Storrs, Connecticut 06269-2157 (United States)

    2014-03-10

    Quantitative phase contrast microscopy reveals thickness or height information of a biological or technical micro-object under investigation. The information obtained from this process provides a means to study their dynamics. Digital holographic (DH) microscopy is one of the most used, state of the art single-shot quantitative techniques for three dimensional imaging of living cells. Conventional off axis DH microscopy directly provides phase contrast images of the objects. However, this process requires two separate beams and their ratio adjustment for high contrast interference fringes. Also the use of two separate beams may make the system more vulnerable to vibrations. Single beam techniques can overcome these hurdles while remaining compact as well. Here, we describe the development of a single beam DH microscope providing whole field imaging of micro-objects. A hologram of the magnified object projected on to a diffuser co-located with a pinhole is recorded with the use of a commercially available diode laser and an arrayed sensor. A Fourier transform of the recorded hologram directly yields the complex amplitude at the image plane. The method proposed was investigated using various phase objects. It was also used to image the dynamics of human red blood cells in which sub-micrometer level thickness variation were measurable.

  19. Accurate reconstruction in digital holographic microscopy using antialiasing shift-invariant contourlet transform

    Science.gov (United States)

    Zhang, Xiaolei; Zhang, Xiangchao; Xu, Min; Zhang, Hao; Jiang, Xiangqian

    2018-03-01

    The measurement of microstructured components is a challenging task in optical engineering. Digital holographic microscopy has attracted intensive attention due to its remarkable capability of measuring complex surfaces. However, speckles arise in the recorded interferometric holograms, and they will degrade the reconstructed wavefronts. Existing speckle removal methods suffer from the problems of frequency aliasing and phase distortions. A reconstruction method based on the antialiasing shift-invariant contourlet transform (ASCT) is developed. Salient edges and corners have sparse representations in the transform domain of ASCT, and speckles can be recognized and removed effectively. As subsampling in the scale and directional filtering schemes is avoided, the problems of frequency aliasing and phase distortions occurring in the conventional multiscale transforms can be effectively overcome, thereby improving the accuracy of wavefront reconstruction. As a result, the proposed method is promising for the digital holographic measurement of complex structures.

  20. Progress in high-resolution x-ray holographic microscopy

    International Nuclear Information System (INIS)

    Jacobsen, C.; Kirz, J.; Howells, M.; McQuaid, K.; Rothman, S.; Feder, R.; Sayre, D.

    1987-07-01

    Among the various types of x-ray microscopes that have been demonstrated, the holographic microscope has had the largest gap between promise and performance. The difficulties of fabricating x-ray optical elements have led some to view holography as the most attractive method for obtaining the ultimate in high resolution x-ray micrographs; however, we know of no investigations prior to 1987 that clearly demonstrated submicron resolution in reconstructed images. Previous efforts suffered from problems such as limited resolution and dynamic range in the recording media, low coherent x-ray flux, and aberrations and diffraction limits in visible light reconstruction. We have addressed the recording limitations through the use of an undulator x-ray source and high-resolution photoresist recording media. For improved results in the readout and reconstruction steps, we have employed metal shadowing and transmission electron microscopy, along with numerical reconstruction techniques. We believe that this approach will allow holography to emerge as a practical method of high-resolution x-ray microscopy. 30 refs., 4 figs

  1. Progress in high-resolution x-ray holographic microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Jacobsen, C.; Kirz, J.; Howells, M.; McQuaid, K.; Rothman, S.; Feder, R.; Sayre, D.

    1987-07-01

    Among the various types of x-ray microscopes that have been demonstrated, the holographic microscope has had the largest gap between promise and performance. The difficulties of fabricating x-ray optical elements have led some to view holography as the most attractive method for obtaining the ultimate in high resolution x-ray micrographs; however, we know of no investigations prior to 1987 that clearly demonstrated submicron resolution in reconstructed images. Previous efforts suffered from problems such as limited resolution and dynamic range in the recording media, low coherent x-ray flux, and aberrations and diffraction limits in visible light reconstruction. We have addressed the recording limitations through the use of an undulator x-ray source and high-resolution photoresist recording media. For improved results in the readout and reconstruction steps, we have employed metal shadowing and transmission electron microscopy, along with numerical reconstruction techniques. We believe that this approach will allow holography to emerge as a practical method of high-resolution x-ray microscopy. 30 refs., 4 figs.

  2. Developments in holographic-based scanner designs

    Science.gov (United States)

    Rowe, David M.

    1997-07-01

    Holographic-based scanning systems have been used for years in the high resolution prepress markets where monochromatic lasers are generally utilized. However, until recently, due to the dispersive properties of holographic optical elements (HOEs), along with the high cost associated with recording 'master' HOEs, holographic scanners have not been able to penetrate major scanning markets such as the laser printer and digital copier markets, low to mid-range imagesetter markets, and the non-contact inspection scanner market. Each of these markets has developed cost effective laser diode based solutions using conventional scanning approaches such as polygon/f-theta lens combinations. In order to penetrate these markets, holographic-based systems must exhibit low cost and immunity to wavelength shifts associated with laser diodes. This paper describes recent developments in the design of holographic scanners in which multiple HOEs, each possessing optical power, are used in conjunction with one curved mirror to passively correct focal plane position errors and spot size changes caused by the wavelength instability of laser diodes. This paper also describes recent advancements in low cost production of high quality HOEs and curved mirrors. Together these developments allow holographic scanners to be economically competitive alternatives to conventional devices in every segment of the laser scanning industry.

  3. Early cell death detection with digital holographic microscopy.

    Directory of Open Access Journals (Sweden)

    Nicolas Pavillon

    Full Text Available BACKGROUND: Digital holography provides a non-invasive measurement of the quantitative phase shifts induced by cells in culture, which can be related to cell volume changes. It has been shown previously that regulation of cell volume, in particular as it relates to ionic homeostasis, is crucially involved in the activation/inactivation of the cell death processes. We thus present here an application of digital holographic microscopy (DHM dedicated to early and label-free detection of cell death. METHODS AND FINDINGS: We provide quantitative measurements of phase signal obtained on mouse cortical neurons, and caused by early neuronal cell volume regulation triggered by excitotoxic concentrations of L-glutamate. We show that the efficiency of this early regulation of cell volume detected by DHM, is correlated with the occurrence of subsequent neuronal death assessed with the widely accepted trypan blue method for detection of cell viability. CONCLUSIONS: The determination of the phase signal by DHM provides a simple and rapid optical method for the early detection of cell death.

  4. Structural properties of liposomes from digital holographic microscopy

    Science.gov (United States)

    Di Maio, Isabelle L.; Carl, Daniel; Langehanenberg, Patrik; Valenzuela, Stella M.; Battle, Andrew R.; Al Khazaaly, Sabah; Killingsworth, Murray; Kemper, Bjorn; von Bally, Gert; Martin, Donald K.

    2006-01-01

    We have constructed liposomes from L alpha Phosphatidylcholine (PC) lipids, which are biomimetic lipids similar to those present in the membranes of mammalian cells. We propose an advance in the use of liposomes, such as for drug delivery, to incorporate into the liposomal membranes transport proteins that have been extracted from the lipid membranes of mammalian cells. In this paper, we describe the usage of a novel optical microscope to characterize the nanomechanical properties of these liposomes. We have applied the technique of digital holographic microscopy, using an instrument recently developed at the University of Münster, Germany. This system enabled us to measure quantitatively the structural changes in liposomes. We have investigated the deformations of these biomimetic lipids comprising these liposomes by applying osmotic stresses, in order to gain insight into the membrane environment prior to incorporation of cloned membrane transport proteins. This control of the nanomechanical properties is important in the stresses transmitted to mechanosensitive ion channels that we have incorporated into the liposomal membranes. These liposomes provide transporting vesicles that respond to mechanical stresses, such as those that occur during implantation.

  5. Applications of holographic on-chip microscopy (Conference Presentation)

    Science.gov (United States)

    Ozcan, Aydogan

    2017-02-01

    My research focuses on the use of computation/algorithms to create new optical microscopy, sensing, and diagnostic techniques, significantly improving existing tools for probing micro- and nano-objects while also simplifying the designs of these analysis tools. In this presentation, I will introduce a set of computational microscopes which use lens-free on-chip imaging to replace traditional lenses with holographic reconstruction algorithms. Basically, 3D images of specimens are reconstructed from their "shadows" providing considerably improved field-of-view (FOV) and depth-of-field, thus enabling large sample volumes to be rapidly imaged, even at nanoscale. These new computational microscopes routinely generate benefit of this technology is that it lends itself to field-portable and cost-effective designs which easily integrate with smartphones to conduct giga-pixel tele-pathology and microscopy even in resource-poor and remote settings where traditional techniques are difficult to implement and sustain, thus opening the door to various telemedicine applications in global health. Through the development of similar computational imagers, I will also report the discovery of new 3D swimming patterns observed in human and animal sperm. One of this newly discovered and extremely rare motion is in the form of "chiral ribbons" where the planar swings of the sperm head occur on an osculating plane creating in some cases a helical ribbon and in some others a twisted ribbon. Shedding light onto the statistics and biophysics of various micro-swimmers' 3D motion, these results provide an important example of how biomedical imaging significantly benefits from emerging computational algorithms/theories, revolutionizing existing tools for observing various micro- and nano-scale phenomena in innovative, high-throughput, and yet cost-effective ways.

  6. Automated classification of cell morphology by coherence-controlled holographic microscopy.

    Science.gov (United States)

    Strbkova, Lenka; Zicha, Daniel; Vesely, Pavel; Chmelik, Radim

    2017-08-01

    In the last few years, classification of cells by machine learning has become frequently used in biology. However, most of the approaches are based on morphometric (MO) features, which are not quantitative in terms of cell mass. This may result in poor classification accuracy. Here, we study the potential contribution of coherence-controlled holographic microscopy enabling quantitative phase imaging for the classification of cell morphologies. We compare our approach with the commonly used method based on MO features. We tested both classification approaches in an experiment with nutritionally deprived cancer tissue cells, while employing several supervised machine learning algorithms. Most of the classifiers provided higher performance when quantitative phase features were employed. Based on the results, it can be concluded that the quantitative phase features played an important role in improving the performance of the classification. The methodology could be valuable help in refining the monitoring of live cells in an automated fashion. We believe that coherence-controlled holographic microscopy, as a tool for quantitative phase imaging, offers all preconditions for the accurate automated analysis of live cell behavior while enabling noninvasive label-free imaging with sufficient contrast and high-spatiotemporal phase sensitivity. (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).

  7. Automated classification of cell morphology by coherence-controlled holographic microscopy

    Science.gov (United States)

    Strbkova, Lenka; Zicha, Daniel; Vesely, Pavel; Chmelik, Radim

    2017-08-01

    In the last few years, classification of cells by machine learning has become frequently used in biology. However, most of the approaches are based on morphometric (MO) features, which are not quantitative in terms of cell mass. This may result in poor classification accuracy. Here, we study the potential contribution of coherence-controlled holographic microscopy enabling quantitative phase imaging for the classification of cell morphologies. We compare our approach with the commonly used method based on MO features. We tested both classification approaches in an experiment with nutritionally deprived cancer tissue cells, while employing several supervised machine learning algorithms. Most of the classifiers provided higher performance when quantitative phase features were employed. Based on the results, it can be concluded that the quantitative phase features played an important role in improving the performance of the classification. The methodology could be valuable help in refining the monitoring of live cells in an automated fashion. We believe that coherence-controlled holographic microscopy, as a tool for quantitative phase imaging, offers all preconditions for the accurate automated analysis of live cell behavior while enabling noninvasive label-free imaging with sufficient contrast and high-spatiotemporal phase sensitivity.

  8. A pocket device for high-throughput optofluidic holographic microscopy

    Science.gov (United States)

    Mandracchia, B.; Bianco, V.; Wang, Z.; Paturzo, M.; Bramanti, A.; Pioggia, G.; Ferraro, P.

    2017-06-01

    Here we introduce a compact holographic microscope embedded onboard a Lab-on-a-Chip (LoC) platform. A wavefront division interferometer is realized by writing a polymer grating onto the channel to extract a reference wave from the object wave impinging the LoC. A portion of the beam reaches the samples flowing along the channel path, carrying their information content to the recording device, while one of the diffraction orders from the grating acts as an off-axis reference wave. Polymeric micro-lenses are delivered forward the chip by Pyro-ElectroHydroDynamic (Pyro-EHD) inkjet printing techniques. Thus, all the required optical components are embedded onboard a pocket device, and fast, non-iterative, reconstruction algorithms can be used. We use our device in combination with a novel high-throughput technique, named Space-Time Digital Holography (STDH). STDH exploits the samples motion inside microfluidic channels to obtain a synthetic hologram, mapped in a hybrid space-time domain, and with intrinsic useful features. Indeed, a single Linear Sensor Array (LSA) is sufficient to build up a synthetic representation of the entire experiment (i.e. the STDH) with unlimited Field of View (FoV) along the scanning direction, independently from the magnification factor. The throughput of the imaging system is dramatically increased as STDH provides unlimited FoV, refocusable imaging of samples inside the liquid volume with no need for hologram stitching. To test our embedded STDH microscopy module, we counted, imaged and tracked in 3D with high-throughput red blood cells moving inside the channel volume under non ideal flow conditions.

  9. 3D measurements of live cells via digital holographic microscopy and terahertz spectroscopy

    Science.gov (United States)

    Park, Jun Yong; Oser, Dorian; Iapozzuto, Peter; Norbury, Sean; Mahajan, Supriya; Khmaladze, Alexander; Sharikova, Anna

    2016-03-01

    This is a study of the central nervous system (CNS) cells, including brain micro vascular endothelial cells (BMV) that constitute the blood brain barrier, and C6 glial cells that are the predominant cell in the brain. The cells are exposed to various chemicals by non-invasive, label-free methods. Digital holographic microscopy (DHM) is a technique that records an interference pattern between an object and reference waves, so that the computationally reconstructed holographic image contains both amplitude and phase information, and 3D images are obtained. The measurement of cell cultures by digital holographic microscopy yields information about cell death mechanisms, since these processes are correlated with individual cell volume. Our in-house DHM combines a visible (red) laser source with a conventional microscope base, and LabVIEW-run data processing. Terahertz spectral signatures are associated with structural changes in molecules and provide complementary information about cells. Both CNS cells BMV and C6 cells are treated with the drug "Methamphetamine" (METH), which induces apoptosis in neuronal cells and exhibits decrease in cell volume, a characteristic of cells undergoing apoptosis (induced cell death). METH can cause CNS cell death by cross-talk between mitochondria-, endoplasmic reticulum-, and receptor-mediated apoptotic events, all of which results in drug induced changes in neuroplasticity and significant neuropathology. Doxorubicin (DOX), a popular anticancer drug, is used as a control. We observe that METH treatment resulted in more pronounced cell volume shrinkage in both the BMV and C6 cells, as compared to DOX-induced cell apoptosis.

  10. Induction of morphological changes in death-induced cancer cells monitored by holographic microscopy.

    Science.gov (United States)

    El-Schich, Zahra; Mölder, Anna; Tassidis, Helena; Härkönen, Pirkko; Falck Miniotis, Maria; Gjörloff Wingren, Anette

    2015-03-01

    We are using the label-free technique of holographic microscopy to analyze cellular parameters including cell number, confluence, cellular volume and area directly in the cell culture environment. We show that death-induced cells can be distinguished from untreated counterparts by the use of holographic microscopy, and we demonstrate its capability for cell death assessment. Morphological analysis of two representative cell lines (L929 and DU145) was performed in the culture flasks without any prior cell detachment. The two cell lines were treated with the anti-tumour agent etoposide for 1-3days. Measurements by holographic microscopy showed significant differences in average cell number, confluence, volume and area when comparing etoposide-treated with untreated cells. The cell volume of the treated cell lines was initially increased at early time-points. By time, cells decreased in volume, especially when treated with high doses of etoposide. In conclusion, we have shown that holographic microscopy allows label-free and completely non-invasive morphological measurements of cell growth, viability and death. Future applications could include real-time monitoring of these holographic microscopy parameters in cells in response to clinically relevant compounds. Copyright © 2015 Elsevier Inc. All rights reserved.

  11. Image recovery from defocused 2D fluorescent images in multimodal digital holographic microscopy.

    Science.gov (United States)

    Quan, Xiangyu; Matoba, Osamu; Awatsuji, Yasuhiro

    2017-05-01

    A technique of three-dimensional (3D) intensity retrieval from defocused, two-dimensional (2D) fluorescent images in the multimodal digital holographic microscopy (DHM) is proposed. In the multimodal DHM, 3D phase and 2D fluorescence distributions are obtained simultaneously by an integrated system of an off-axis DHM and a conventional epifluorescence microscopy, respectively. This gives us more information of the target; however, defocused fluorescent images are observed due to the short depth of field. In this Letter, we propose a method to recover the defocused images based on the phase compensation and backpropagation from the defocused plane to the focused plane using the distance information that is obtained from a 3D phase distribution. By applying Zernike polynomial phase correction, we brought back the fluorescence intensity to the focused imaging planes. The experimental demonstration using fluorescent beads is presented, and the expected applications are suggested.

  12. Necrosis and apoptosis pathways of cell death at photodynamic treatment in vitro as revealed by digital holographic microscopy

    Science.gov (United States)

    Semenova, I. V.; Belashov, A. V.; Belyaeva, T. N.; Kornilova, E. S.; Salova, A. V.; Zhikhoreva, A. A.; Vasyutinskii, O. S.

    2018-02-01

    Monitoring of variations in morphological characteristics of cultured HeLa cells after photodynamic treatment with Radachlorin photosensitizer is performed by means of digital holographic microscopy. The observed dose-dependent post-treatment variations of phase shift evidence threshold effect of photodynamic treatment and allow for distinguishing between necrotic or apoptotic pathways of cell death. Results obtained by holographic microscopy were confirmed by means of far-field optical microscopy and confocal fluorescence microscopy with commonly used test assays.

  13. High-throughput characterization of film thickness in thin film materials libraries by digital holographic microscopy

    International Nuclear Information System (INIS)

    Lai Yiuwai; Hofmann, Martin R; Ludwig, Alfred; Krause, Michael; Savan, Alan; Thienhaus, Sigurd; Koukourakis, Nektarios

    2011-01-01

    A high-throughput characterization technique based on digital holography for mapping film thickness in thin-film materials libraries was developed. Digital holographic microscopy is used for fully automatic measurements of the thickness of patterned films with nanometer resolution. The method has several significant advantages over conventional stylus profilometry: it is contactless and fast, substrate bending is compensated, and the experimental setup is simple. Patterned films prepared by different combinatorial thin-film approaches were characterized to investigate and demonstrate this method. The results show that this technique is valuable for the quick, reliable and high-throughput determination of the film thickness distribution in combinatorial materials research. Importantly, it can also be applied to thin films that have been structured by shadow masking.

  14. High-throughput characterization of film thickness in thin film materials libraries by digital holographic microscopy.

    Science.gov (United States)

    Lai, Yiu Wai; Krause, Michael; Savan, Alan; Thienhaus, Sigurd; Koukourakis, Nektarios; Hofmann, Martin R; Ludwig, Alfred

    2011-10-01

    A high-throughput characterization technique based on digital holography for mapping film thickness in thin-film materials libraries was developed. Digital holographic microscopy is used for fully automatic measurements of the thickness of patterned films with nanometer resolution. The method has several significant advantages over conventional stylus profilometry: it is contactless and fast, substrate bending is compensated, and the experimental setup is simple. Patterned films prepared by different combinatorial thin-film approaches were characterized to investigate and demonstrate this method. The results show that this technique is valuable for the quick, reliable and high-throughput determination of the film thickness distribution in combinatorial materials research. Importantly, it can also be applied to thin films that have been structured by shadow masking.

  15. New approaches for the analysis of confluent cell layers with quantitative phase digital holographic microscopy

    Science.gov (United States)

    Pohl, L.; Kaiser, M.; Ketelhut, S.; Pereira, S.; Goycoolea, F.; Kemper, Björn

    2016-03-01

    Digital holographic microscopy (DHM) enables high resolution non-destructive inspection of technical surfaces and minimally-invasive label-free live cell imaging. However, the analysis of confluent cell layers represents a challenge as quantitative DHM phase images in this case do not provide sufficient information for image segmentation, determination of the cellular dry mass or calculation of the cell thickness. We present novel strategies for the analysis of confluent cell layers with quantitative DHM phase contrast utilizing a histogram based-evaluation procedure. The applicability of our approach is illustrated by quantification of drug induced cell morphology changes and it is shown that the method is capable to quantify reliable global morphology changes of confluent cell layers.

  16. Determination of the refractive index of dehydrated cells by means of digital holographic microscopy

    Science.gov (United States)

    Belashov, A. V.; Zhikhoreva, A. A.; Bespalov, V. G.; Vasyutinskii, O. S.; Zhilinskaya, N. T.; Novik, V. I.; Semenova, I. V.

    2017-10-01

    Spatial distributions of the integral refractive index in dehydrated cells of human oral cavity epithelium are obtained by means of digital holographic microscopy, and mean refractive index of the cells is determined. The statistical analysis of the data obtained is carried out, and absolute errors of the method are estimated for different experimental conditions.

  17. Digital holographic microscopy: a novel tool to study the morphology, physiology and ecology of diatoms

    NARCIS (Netherlands)

    Zetsche, E.-M.; El Mallahi, A.; Meysman, F.J.R.

    2016-01-01

    Recent advances in optical components, computational hardware and image analysis algorithms have led to the development of a powerful new imaging tool, digital holographic microscopy (DHM). So far, DHM has been predominantly applied in the life sciences and medical research, and here, we evaluate

  18. Label-free quantitative cell division monitoring of endothelial cells by digital holographic microscopy

    Science.gov (United States)

    Kemper, Björn; Bauwens, Andreas; Vollmer, Angelika; Ketelhut, Steffi; Langehanenberg, Patrik; Müthing, Johannes; Karch, Helge; von Bally, Gert

    2010-05-01

    Digital holographic microscopy (DHM) enables quantitative multifocus phase contrast imaging for nondestructive technical inspection and live cell analysis. Time-lapse investigations on human brain microvascular endothelial cells demonstrate the use of DHM for label-free dynamic quantitative monitoring of cell division of mother cells into daughter cells. Cytokinetic DHM analysis provides future applications in toxicology and cancer research.

  19. Optimized computational imaging methods for small-target sensing in lens-free holographic microscopy

    Science.gov (United States)

    Xiong, Zhen; Engle, Isaiah; Garan, Jacob; Melzer, Jeffrey E.; McLeod, Euan

    2018-02-01

    Lens-free holographic microscopy is a promising diagnostic approach because it is cost-effective, compact, and suitable for point-of-care applications, while providing high resolution together with an ultra-large field-of-view. It has been applied to biomedical sensing, where larger targets like eukaryotic cells, bacteria, or viruses can be directly imaged without labels, and smaller targets like proteins or DNA strands can be detected via scattering labels like micro- or nano-spheres. Automated image processing routines can count objects and infer target concentrations. In these sensing applications, sensitivity and specificity are critically affected by image resolution and signal-to-noise ratio (SNR). Pixel super-resolution approaches have been shown to boost resolution and SNR by synthesizing a high-resolution image from multiple, partially redundant, low-resolution images. However, there are several computational methods that can be used to synthesize the high-resolution image, and previously, it has been unclear which methods work best for the particular case of small-particle sensing. Here, we quantify the SNR achieved in small-particle sensing using regularized gradient-descent optimization method, where the regularization is based on cardinal-neighbor differences, Bayer-pattern noise reduction, or sparsity in the image. In particular, we find that gradient-descent with sparsity-based regularization works best for small-particle sensing. These computational approaches were evaluated on images acquired using a lens-free microscope that we assembled from an off-the-shelf LED array and color image sensor. Compared to other lens-free imaging systems, our hardware integration, calibration, and sample preparation are particularly simple. We believe our results will help to enable the best performance in lens-free holographic sensing.

  20. Multispectral digital lensless holographic microscopy: from femtosecond laser to white light LED

    International Nuclear Information System (INIS)

    Garcia-Sucerquia, J

    2015-01-01

    The use of femtosecond laser radiation and super bright white LED in digital lensless holographic microscopy is presented. For the ultrafast laser radiation two different configurations of operation of the microscope are presented and the dissimilar performance of each one analyzed. The microscope operating with a super bright white light LED in combination with optical filters shows very competitive performance as it is compared with more expensive optical sources. The broadband emission of both radiation sources allows the multispectral imaging of biological samples to obtain spectral responses and/or full color images of the microscopic specimens; sections of the head of a Drosophila melanogaster fly are imaged in this contribution. The simple, solid, compact, lightweight, and reliable architecture of digital lensless holographic microscopy operating with broadband light sources to image biological specimens exhibiting micrometer-sized details is evaluated in the present contribution. (paper)

  1. Dynamics and morphometric characterization of hippocampus neurons using digital holographic microscopy

    Science.gov (United States)

    Elkatlawy, Saeid; Gomariz, María.; Soto-Sánchez, Cristina; Martínez Navarrete, Gema; Fernández, Eduardo; Fimia, Antonio

    2014-05-01

    In this paper we report on the use of digital holographic microscopy for 3D real time imaging of cultured neurons and neural networks, in vitro. Digital holographic microscopy is employed as an assessment tool to study the biophysical origin of neurodegenerative diseases. Our study consists in the morphological characterization of the axon, dendrites and cell bodies. The average size and thickness of the soma were 21 and 13 μm, respectively. Furthermore, the average size and diameter of some randomly selected neurites were 4.8 and 0.89 μm, respectively. In addition, the spatiotemporal growth process of cellular bodies and extensions was fitted to by a non-linear behavior of the nerve system. Remarkably, this non-linear process represents the relationship between the growth process of cellular body with respect to the axon and dendrites of the neurons.

  2. Realization of an optical interferometer based on holographic optics ...

    Indian Academy of Sciences (India)

    The paper describes a simple and cost effective method for the realization of an optical interferometer based on holographic optics, which use minimal bulk optical components. The optical arrangement in the proposed method involves a very simple alignment procedure and inexpensive holographic recording material is ...

  3. Digital holographic microscopy for toxicity testing and cell culture quality control

    Science.gov (United States)

    Kemper, Björn

    2018-02-01

    For the example of digital holographic microscopy (DHM), it is illustrated how label-free biophysical parameter sets can be extracted from quantitative phase images of adherent and suspended cells, and how the retrieved data can be applied for in-vitro toxicity testing and cell culture quality assessment. This includes results from the quantification of the reactions of cells to toxic substances as well as data from sophisticated monitoring of cell alterations that are related to changes of cell culture conditions.

  4. Studying the dynamics of colloidal particles with digital holographic microscopy and electromagnetic scattering solutions

    Directory of Open Access Journals (Sweden)

    V. N. Manoharan

    2011-09-01

    Full Text Available Digital holographic microscopy (DHM can measure the 3D positions as well as the scattering properties of colloidal particles in a single 2D image. We describe DHM and our analysis of recorded holograms with exact scattering solutions, which permit the measurement of 3D particle positions with ∼10 nm precision and millisecond time resolution, and discuss studies of the Brownian dynamics of clusters of spheres with DHM.

  5. Ferroelectric domain pattern in barium titanate single crystals studied by means of digital holographic microscopy

    Czech Academy of Sciences Publication Activity Database

    Mokrý, Pavel; Psota, Pavel; Steiger, Kateřina; Václavík, Jan; Doleček, Roman; Vápenka, David; Lédl, Vít

    2016-01-01

    Roč. 49, č. 25 (2016), č. článku 255307. ISSN 0022-3727 R&D Projects: GA ČR(CZ) GA14-32228S Institutional support: RVO:61389021 Keywords : ferroelectric domain patterns * electro-optical materials * digital holographic microscopy Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 2.588, year: 2016 http://iopscience.iop.org/article/10.1088/0022-3727/49/25/255307

  6. Near real-time digital holographic microscope based on GPU parallel computing

    Science.gov (United States)

    Zhu, Gang; Zhao, Zhixiong; Wang, Huarui; Yang, Yan

    2018-01-01

    A transmission near real-time digital holographic microscope with in-line and off-axis light path is presented, in which the parallel computing technology based on compute unified device architecture (CUDA) and digital holographic microscopy are combined. Compared to other holographic microscopes, which have to implement reconstruction in multiple focal planes and are time-consuming the reconstruction speed of the near real-time digital holographic microscope can be greatly improved with the parallel computing technology based on CUDA, so it is especially suitable for measurements of particle field in micrometer and nanometer scale. Simulations and experiments show that the proposed transmission digital holographic microscope can accurately measure and display the velocity of particle field in micrometer scale, and the average velocity error is lower than 10%.With the graphic processing units(GPU), the computing time of the 100 reconstruction planes(512×512 grids) is lower than 120ms, while it is 4.9s using traditional reconstruction method by CPU. The reconstruction speed has been raised by 40 times. In other words, it can handle holograms at 8.3 frames per second and the near real-time measurement and display of particle velocity field are realized. The real-time three-dimensional reconstruction of particle velocity field is expected to achieve by further optimization of software and hardware. Keywords: digital holographic microscope,

  7. Ex-vivo holographic microscopy and spectroscopic analysis of head and neck cancer

    Science.gov (United States)

    Holler, Stephen; Wurtz, Robert; Auyeung, Kelsey; Auyeung, Kris; Paspaley-Grbavac, Milan; Mulroe, Brigid; Sobrero, Maximiliano; Miles, Brett

    2015-03-01

    Optical probes to identify tumor margins in vivo would greatly reduce the time, effort and complexity in the surgical removal of malignant tissue in head and neck cancers. Current approaches involve visual microscopy of stained tissue samples to determine cancer margins, which results in the excision of excess of tissue to assure complete removal of the cancer. Such surgical procedures and follow-on chemotherapy can adversely affect the patient's recovery and subsequent quality of life. In order to reduce the complexity of the process and minimize adverse effects on the patient, we investigate ex vivo tissue samples (stained and unstained) using digital holographic microscopy in conjunction with spectroscopic analyses (reflectance and transmission spectroscopy) in order to determine label-free, optically identifiable characteristic features that may ultimately be used for in vivo processing of cancerous tissues. The tissue samples studied were squamous cell carcinomas and associated controls from patients of varying age, gender and race. Holographic microscopic imaging scans across both cancerous and non-cancerous tissue samples yielded amplitude and phase reconstructions that were correlated with spectral signatures. Though the holographic reconstructions and measured spectra indicate variations even among the same class of tissue, preliminary results indicate the existence of some discriminating features. Further analyses are presently underway to further this work and extract additional information from the imaging and spectral data that may prove useful for in vivo surgical identification.

  8. Three-dimensional motion measurements of free-swimming microorganisms using digital holographic microscopy

    International Nuclear Information System (INIS)

    Lee, Sang Joon; Seo, Kyung Won; Choi, Yong Seok; Sohn, Myong Hwan

    2011-01-01

    A digital holographic microscope is employed to measure the 3D motion of free-swimming microorganisms. The focus function used to quantify image sharpness provides a better depth-directional accuracy with a smaller depth-of-focus compared with the intensity method in determining the depth-directional position of spherical particles of various diameters. The focus function is then applied to measure the 3D positions of free-swimming microorganisms, namely dinoflagellates C. polykrikoides and P. minimum. Both automatic segmentation and proper selection of a focus function for a selected segment are important processes in measuring the positional information of two free-swimming microorganisms of different shapes with various width-to-length ratios. The digital holographic microscopy technique improved in this work is useful for measuring 3D swimming trajectories, velocities and attitudes of hundreds of microorganisms simultaneously. It also exhibits exceptional depth-directional accuracy

  9. Reduction of parasitic interferences in digital holographic microscopy by numerically decreased coherence length

    Science.gov (United States)

    Kosmeier, S.; Langehanenberg, P.; von Bally, G.; Kemper, B.

    2012-01-01

    Due to the large coherence length of laser light, optical path length (OPL) resolution in laser based digital holographic microscopy suffers from parasitic interferences caused by multiple reflections within the experimental setup. Use of partially coherent light reduces this drawback but requires precise and stable matching of object and reference arm's OPLs and limits the spatial frequency of the interference pattern in off-axis holography. Here, we investigate if the noise properties of spectrally broadened light sources can be generated numerically. Therefore, holograms are coherently captured at different laser wavelengths and the corresponding reconstructed wave fields are numerically superimposed utilizing variable weightings. Gaussian and rectangular spectral shapes of the so synthesized field are analyzed with respect to the resulting noise level, which is quantified in OPL distributions of a reflective test target. Utilizing a Gaussian weighting, the noise level is found to be similar to the one obtained with the partially coherent light of a superluminescent diode. With a rectangular shaped synthesized spectrum, noise is reduced more efficient than with a Gaussian one. The applicability of the method in label-free cell analysis is demonstrated by quantitative phase contrast images obtained from living cancer cells.

  10. Coherent noise reduction in digital holographic microscopy by averaging multiple holograms recorded with a multimode laser.

    Science.gov (United States)

    Pan, Feng; Yang, Lizhi; Xiao, Wen

    2017-09-04

    In digital holographic microscopy (DHM), it is undesirable to observe coherent noise in the reconstructed images. The sources of the noise are mainly the parasitic interference fringes caused by multiple reflections and the speckle pattern caused by the optical scattering on the object surface. Here we propose a noise reduction approach in DHM by averaging multiple holograms recorded with a multimode laser. Based on the periodicity of the temporal coherence of a multimode semiconductor laser, we acquire a series of holograms by changing the optical path length difference between the reference beam and object beam. Because of the use of low coherence light, we can remove the parasitic interference fringes caused by multiple reflections in the holograms. In addition, the coherent noise patterns change in this process due to the different optical paths. Therefore, the coherent noise can be reduced by averaging the multiple reconstructions with uncorrelated noise patterns. Several experiments have been carried out to validate the effectiveness of the proposed approach for coherent noise reduction in DHM. It is shown a remarkable improvement both in amplitude imaging quality and phase measurement accuracy.

  11. Sequential processing of quantitative phase images for the study of cell behaviour in real-time digital holographic microscopy.

    Science.gov (United States)

    Zikmund, T; Kvasnica, L; Týč, M; Křížová, A; Colláková, J; Chmelík, R

    2014-11-01

    Transmitted light holographic microscopy is particularly used for quantitative phase imaging of transparent microscopic objects such as living cells. The study of the cell is based on extraction of the dynamic data on cell behaviour from the time-lapse sequence of the phase images. However, the phase images are affected by the phase aberrations that make the analysis particularly difficult. This is because the phase deformation is prone to change during long-term experiments. Here, we present a novel algorithm for sequential processing of living cells phase images in a time-lapse sequence. The algorithm compensates for the deformation of a phase image using weighted least-squares surface fitting. Moreover, it identifies and segments the individual cells in the phase image. All these procedures are performed automatically and applied immediately after obtaining every single phase image. This property of the algorithm is important for real-time cell quantitative phase imaging and instantaneous control of the course of the experiment by playback of the recorded sequence up to actual time. Such operator's intervention is a forerunner of process automation derived from image analysis. The efficiency of the propounded algorithm is demonstrated on images of rat fibrosarcoma cells using an off-axis holographic microscope. © 2014 The Authors Journal of Microscopy © 2014 Royal Microscopical Society.

  12. Quantitative assessment of cancer cell morphology and motility using telecentric digital holographic microscopy and machine learning.

    Science.gov (United States)

    Lam, Van K; Nguyen, Thanh C; Chung, Byung M; Nehmetallah, George; Raub, Christopher B

    2018-03-01

    The noninvasive, fast acquisition of quantitative phase maps using digital holographic microscopy (DHM) allows tracking of rapid cellular motility on transparent substrates. On two-dimensional surfaces in vitro, MDA-MB-231 cancer cells assume several morphologies related to the mode of migration and substrate stiffness, relevant to mechanisms of cancer invasiveness in vivo. The quantitative phase information from DHM may accurately classify adhesive cancer cell subpopulations with clinical relevance. To test this, cells from the invasive breast cancer MDA-MB-231 cell line were cultured on glass, tissue-culture treated polystyrene, and collagen hydrogels, and imaged with DHM followed by epifluorescence microscopy after staining F-actin and nuclei. Trends in cell phase parameters were tracked on the different substrates, during cell division, and during matrix adhesion, relating them to F-actin features. Support vector machine learning algorithms were trained and tested using parameters from holographic phase reconstructions and cell geometric features from conventional phase images, and used to distinguish between elongated and rounded cell morphologies. DHM was able to distinguish between elongated and rounded morphologies of MDA-MB-231 cells with 94% accuracy, compared to 83% accuracy using cell geometric features from conventional brightfield microscopy. This finding indicates the potential of DHM to detect and monitor cancer cell morphologies relevant to cell cycle phase status, substrate adhesion, and motility. © 2017 International Society for Advancement of Cytometry. © 2017 International Society for Advancement of Cytometry.

  13. X-ray holographic microscopy experiments at the Brookhaven synchrotron light source

    International Nuclear Information System (INIS)

    Howells, M.R.; Iarocci, M.; Kenney, J.; Kirz, J.; Rarback, H.

    1983-01-01

    Soft x-ray holographic microscopy is discussed from an experimental point of view. Three series of measurements have been carried out using the Brookhaven 750 MeV storage ring as an x-ray source. Young slits fringes, Gabor (in line) holograms and various data pertaining to the soft x-ray performance of photographic plates are reported. The measurements are discussed in terms of the technique for recording them and the experimental limitations in effect. Some discussion is also given of the issues involved in reconstruction using visible light

  14. Digital Holographic Microscopy, a Method for Detection of Microorganisms in Plume Samples from Enceladus and Other Icy Worlds.

    Science.gov (United States)

    Bedrossian, Manuel; Lindensmith, Chris; Nadeau, Jay L

    2017-09-01

    Detection of extant microbial life on Earth and elsewhere in the Solar System requires the ability to identify and enumerate micrometer-scale, essentially featureless cells. On Earth, bacteria are usually enumerated by culture plating or epifluorescence microscopy. Culture plates require long incubation times and can only count culturable strains, and epifluorescence microscopy requires extensive staining and concentration of the sample and instrumentation that is not readily miniaturized for space. Digital holographic microscopy (DHM) represents an alternative technique with no moving parts and higher throughput than traditional microscopy, making it potentially useful in space for detection of extant microorganisms provided that sufficient numbers of cells can be collected. Because sample collection is expected to be the limiting factor for space missions, especially to outer planets, it is important to quantify the limits of detection of any proposed technique for extant life detection. Here we use both laboratory and field samples to measure the limits of detection of an off-axis digital holographic microscope (DHM). A statistical model is used to estimate any instrument's probability of detection at various bacterial concentrations based on the optical performance characteristics of the instrument, as well as estimate the confidence interval of detection. This statistical model agrees well with the limit of detection of 10 3 cells/mL that was found experimentally with laboratory samples. In environmental samples, active cells were immediately evident at concentrations of 10 4 cells/mL. Published estimates of cell densities for Enceladus plumes yield up to 10 4 cells/mL, which are well within the off-axis DHM's limits of detection to confidence intervals greater than or equal to 95%, assuming sufficient sample volumes can be collected. The quantitative phase imaging provided by DHM allowed minerals to be distinguished from cells. Off-axis DHM's ability for

  15. Single-shot full resolution region-of-interest (ROI) reconstruction in image plane digital holographic microscopy

    Science.gov (United States)

    Singh, Mandeep; Khare, Kedar

    2018-05-01

    We describe a numerical processing technique that allows single-shot region-of-interest (ROI) reconstruction in image plane digital holographic microscopy with full pixel resolution. The ROI reconstruction is modelled as an optimization problem where the cost function to be minimized consists of an L2-norm squared data fitting term and a modified Huber penalty term that are minimized alternately in an adaptive fashion. The technique can provide full pixel resolution complex-valued images of the selected ROI which is not possible to achieve with the commonly used Fourier transform method. The technique can facilitate holographic reconstruction of individual cells of interest from a large field-of-view digital holographic microscopy data. The complementary phase information in addition to the usual absorption information already available in the form of bright field microscopy can make the methodology attractive to the biomedical user community.

  16. Automatic cell identification and visualization using digital holographic microscopy with head mounted augmented reality devices.

    Science.gov (United States)

    O'Connor, Timothy; Rawat, Siddharth; Markman, Adam; Javidi, Bahram

    2018-03-01

    We propose a compact imaging system that integrates an augmented reality head mounted device with digital holographic microscopy for automated cell identification and visualization. A shearing interferometer is used to produce holograms of biological cells, which are recorded using customized smart glasses containing an external camera. After image acquisition, segmentation is performed to isolate regions of interest containing biological cells in the field-of-view, followed by digital reconstruction of the cells, which is used to generate a three-dimensional (3D) pseudocolor optical path length profile. Morphological features are extracted from the cell's optical path length map, including mean optical path length, coefficient of variation, optical volume, projected area, projected area to optical volume ratio, cell skewness, and cell kurtosis. Classification is performed using the random forest classifier, support vector machines, and K-nearest neighbor, and the results are compared. Finally, the augmented reality device displays the cell's pseudocolor 3D rendering of its optical path length profile, extracted features, and the identified cell's type or class. The proposed system could allow a healthcare worker to quickly visualize cells using augmented reality smart glasses and extract the relevant information for rapid diagnosis. To the best of our knowledge, this is the first report on the integration of digital holographic microscopy with augmented reality devices for automated cell identification and visualization.

  17. Digital stereo-holographic microscopy for studying three-dimensional particle dynamics

    Science.gov (United States)

    Byeon, Hyeokjun; Go, Taesik; Lee, Sang Joon

    2018-06-01

    A digital stereo-holographic microscopy (DsHM) with two viewing angles is proposed to measure 3D information of microscale particles. This approach includes two volumetric recordings and numerical reconstruction, and it involves the combination of separately reconstructed holograms. The 3D positional information of a particle was determined by searching the center of the overlapped reconstructed volume. After confirming the proposed technique using static spherical particles, the 3D information of moving particles suspended in a Hagen-Poiseiulle flow was successfully obtained. Moreover, the 3D information of nonspherical particles, including ellipsoidal particles and red blood cells, were measured using the proposed technique. In addition to 3D positional information, the orientation and shape of the test samples were obtained from the plane images by slicing the overlapped volume perpendicular to the directions of the image recordings. This DsHM technique will be useful in analyzing the 3D dynamic behavior of various nonspherical particles, which cannot be measured by conventional digital holographic microscopy.

  18. Short-coherence in-line phase-shifting infrared digital holographic microscopy for measurement of internal structure in silicon

    Science.gov (United States)

    Xi, Teli; Dou, Jiazhen; Di, Jianglei; Li, Ying; Zhang, Jiwei; Ma, Chaojie; Zhao, Jianlin

    2017-06-01

    Short-coherence in-line phase-shifting digital holographic microscopy based on Michelson interferometer is proposed to measure internal structure in silicon. In the configuration, a short-coherence infrared laser is used as the light source in order to avoid the interference formed by the reference wave and the reflected wave from the front surface of specimen. At the same time, in-line phase-shifting configuration is introduced to overcome the problem of poor resolution and large pixel size of the infrared camera and improve the space bandwidth product of the system. A specimen with staircase structure is measured by using the proposed configuration and the 3D shape distribution are given to verify the effectiveness and accuracy of the method.

  19. Future of photorefractive based holographic 3D display

    Science.gov (United States)

    Blanche, P.-A.; Bablumian, A.; Voorakaranam, R.; Christenson, C.; Lemieux, D.; Thomas, J.; Norwood, R. A.; Yamamoto, M.; Peyghambarian, N.

    2010-02-01

    The very first demonstration of our refreshable holographic display based on photorefractive polymer was published in Nature early 20081. Based on the unique properties of a new organic photorefractive material and the holographic stereography technique, this display addressed a gap between large static holograms printed in permanent media (photopolymers) and small real time holographic systems like the MIT holovideo. Applications range from medical imaging to refreshable maps and advertisement. Here we are presenting several technical solutions for improving the performance parameters of the initial display from an optical point of view. Full color holograms can be generated thanks to angular multiplexing, the recording time can be reduced from minutes to seconds with a pulsed laser, and full parallax hologram can be recorded in a reasonable time thanks to parallel writing. We also discuss the future of such a display and the possibility of video rate.

  20. Investigation on cytoskeleton dynamics for no-adherent cells subjected to point-like stimuli by digital holographic microscopy and holographic optical trapping

    Science.gov (United States)

    Miccio, Lisa; Merola, Francesco; Memmolo, Pasquale; Mugnano, Martina; Fusco, Sabato; Netti, Paolo A.; Ferraro, Pietro

    2014-05-01

    Guiding, controlling and studying cellular functions are challenging themes in the biomedical field, as they are fundamental prerequisites for new therapeutic strategies from tissue regeneration to controlled drug delivery. In recent years, multidisciplinary studies in nanotechnology offer new tools to investigate important biophysical phenomena in response to the local physical characteristics of the extracellular environment, some examples are the mechanisms of cell adhesion, migration, communication and differentiation. Indeed for reproducing the features of the extracellular matrix in vitro, it is essential to develop active devices that evoke as much as possible the natural cellular environment. Our investigation is in the framework of studying and clarifying the biophysical mechanisms of the interaction between cells and the microenvironment in which they exist. We implement an optical tweezers setup to investigate cell material interaction and we use Digital Holography as non-invasive imaging technique in microscopy. We exploit Holographic Optical Tweezers arrangement in order to trap and manage functionalized micrometric latex beads to induce mechanical deformation in suspended cells. A lot of papers in literature examine the dynamics of the cytoskeleton when cells adhere on substrates and nowadays well established cell models are based on such research activities. Actually, the natural cell environment is made of a complex extracellular matrix and the single cell behavior is due to intricate interactions with the environment and are strongly correlated to the cell-cell interactions. Our investigation is devoted to understand the inner cell mechanism when it is mechanically stressed by point-like stimulus without the substrate influence.

  1. Micro patterned surfaces: an effective tool for long term digital holographic microscopy cell imaging

    Science.gov (United States)

    Mues, Sarah; Lilge, Inga; Schönherr, Holger; Kemper, Björn; Schnekenburger, Jürgen

    2017-02-01

    The major problem of Digital Holographic Microscopy (DHM) long term live cell imaging is that over time most of the tracked cells move out of the image area and other ones move in. Therefore, most of the cells are lost for the evaluation of individual cellular processes. Here, we present an effective solution for this crucial problem of long-term microscopic live cell analysis. We have generated functionalized slides containing areas of 250 μm per 200 μm. These micropatterned biointerfaces consist of passivating polyaclrylamide brushes (PAAm). Inner areas are backfilled with octadecanthiol (ODT), which allows cell attachment. The fouling properties of these surfaces are highly controllable and therefore the defined areas designed for the size our microscopic image areas were effective in keeping all cells inside the rectangles over the selected imaging period.

  2. Radial super-resolution in digital holographic microscopy using structured illumination with circular symmetry

    Science.gov (United States)

    Yin, Yujian; Su, Ping; Ma, Jianshe

    2018-01-01

    A method to improve the radial resolution using special structured light is proposed in the field of digital holographic microscopy (DHM). A specimen is illuminated with circular symmetrical structured light that makes the spectrum have radial movement, so that high frequency components of the specimen are moved into the passband of the receiver to overcome the diffraction limit. In the DHM imaging system, Computer Generated Hologram (CGH) technology is used to generate the required structured light grating. Then the grating is loaded into a spatial light modulator (SLM) to obtain specific structured illumination. After recording the hologram, digital reconstruction, for the microstructure of a binary optical element that needs to observe radial distribution, the radial resolution of the specimen is improved experimentally compare it with the result of one-dimensional sinusoidal structured light imaging. And a method of designing structured light is presented.

  3. Correction of phase-shifting error in wavelength scanning digital holographic microscopy

    Science.gov (United States)

    Zhang, Xiaolei; Wang, Jie; Zhang, Xiangchao; Xu, Min; Zhang, Hao; Jiang, Xiangqian

    2018-05-01

    Digital holographic microscopy is a promising method for measuring complex micro-structures with high slopes. A quasi-common path interferometric apparatus is adopted to overcome environmental disturbances, and an acousto-optic tunable filter is used to obtain multi-wavelength holograms. However, the phase shifting error caused by the acousto-optic tunable filter reduces the measurement accuracy and, in turn, the reconstructed topographies are erroneous. In this paper, an accurate reconstruction approach is proposed. It corrects the phase-shifting errors by minimizing the difference between the ideal interferograms and the recorded ones. The restriction on the step number and uniformity of the phase shifting is relaxed in the interferometry, and the measurement accuracy for complex surfaces can also be improved. The universality and superiority of the proposed method are demonstrated by practical experiments and comparison to other measurement methods.

  4. A holographic waveguide based eye tracker

    Science.gov (United States)

    Liu, Changgeng; Pazzucconi, Beatrice; Liu, Juan; Liu, Lei; Yao, Xincheng

    2018-02-01

    We demonstrated the feasibility of using holographic waveguide for eye tracking. A custom-built holographic waveguide, a 20 mm x 60 mm x 3 mm flat glass substrate with integrated in- and out-couplers, was used for the prototype development. The in- and out-couplers, photopolymer films with holographic fringes, induced total internal reflection in the glass substrate. Diffractive optical elements were integrated into the in-coupler to serve as an optical collimator. The waveguide captured images of the anterior segment of the eye right in front of it and guided the images to a processing unit distant from the eye. The vector connecting the pupil center (PC) and the corneal reflex (CR) of the eye was used to compute eye position in the socket. An eye model, made of a high quality prosthetic eye, was used prototype validation. The benchtop prototype demonstrated a linear relationship between the angular eye position and the PC/CR vector over a range of 60 horizontal degrees and 30 vertical degrees at a resolution of 0.64-0.69 degrees/pixel by simple pixel count. The uncertainties of the measurements at different angular positions were within 1.2 pixels, which indicated that the prototype exhibited a high level of repeatability. These results confirmed that the holographic waveguide technology could be a feasible platform for developing a wearable eye tracker. Further development can lead to a compact, see-through eye tracker, which allows continuous monitoring of eye movement during real life tasks, and thus benefits diagnosis of oculomotor disorders.

  5. Movies of cellular and sub-cellular motion by digital holographic microscopy

    Directory of Open Access Journals (Sweden)

    Yu Lingfeng

    2006-03-01

    Full Text Available Abstract Background Many biological specimens, such as living cells and their intracellular components, often exhibit very little amplitude contrast, making it difficult for conventional bright field microscopes to distinguish them from their surroundings. To overcome this problem phase contrast techniques such as Zernike, Normarsky and dark-field microscopies have been developed to improve specimen visibility without chemically or physically altering them by the process of staining. These techniques have proven to be invaluable tools for studying living cells and furthering scientific understanding of fundamental cellular processes such as mitosis. However a drawback of these techniques is that direct quantitative phase imaging is not possible. Quantitative phase imaging is important because it enables determination of either the refractive index or optical thickness variations from the measured optical path length with sub-wavelength accuracy. Digital holography is an emergent phase contrast technique that offers an excellent approach in obtaining both qualitative and quantitative phase information from the hologram. A CCD camera is used to record a hologram onto a computer and numerical methods are subsequently applied to reconstruct the hologram to enable direct access to both phase and amplitude information. Another attractive feature of digital holography is the ability to focus on multiple focal planes from a single hologram, emulating the focusing control of a conventional microscope. Methods A modified Mach-Zender off-axis setup in transmission is used to record and reconstruct a number of holographic amplitude and phase images of cellular and sub-cellular features. Results Both cellular and sub-cellular features are imaged with sub-micron, diffraction-limited resolution. Movies of holographic amplitude and phase images of living microbes and cells are created from a series of holograms and reconstructed with numerically adjustable

  6. Three-dimensional counting of morphologically normal human red blood cells via digital holographic microscopy

    Science.gov (United States)

    Yi, Faliu; Moon, Inkyu; Lee, Yeon H.

    2015-01-01

    Counting morphologically normal cells in human red blood cells (RBCs) is extremely beneficial in the health care field. We propose a three-dimensional (3-D) classification method of automatically determining the morphologically normal RBCs in the phase image of multiple human RBCs that are obtained by off-axis digital holographic microscopy (DHM). The RBC holograms are first recorded by DHM, and then the phase images of multiple RBCs are reconstructed by a computational numerical algorithm. To design the classifier, the three typical RBC shapes, which are stomatocyte, discocyte, and echinocyte, are used for training and testing. Nonmain or abnormal RBC shapes different from the three normal shapes are defined as the fourth category. Ten features, including projected surface area, average phase value, mean corpuscular hemoglobin, perimeter, mean corpuscular hemoglobin surface density, circularity, mean phase of center part, sphericity coefficient, elongation, and pallor, are extracted from each RBC after segmenting the reconstructed phase images by using a watershed transform algorithm. Moreover, four additional properties, such as projected surface area, perimeter, average phase value, and elongation, are measured from the inner part of each cell, which can give significant information beyond the previous 10 features for the separation of the RBC groups; these are verified in the experiment by the statistical method of Hotelling's T-square test. We also apply the principal component analysis algorithm to reduce the dimension number of variables and establish the Gaussian mixture densities using the projected data with the first eight principal components. Consequently, the Gaussian mixtures are used to design the discriminant functions based on Bayesian decision theory. To improve the performance of the Bayes classifier and the accuracy of estimation of its error rate, the leaving-one-out technique is applied. Experimental results show that the proposed method can

  7. Lensless digital holographic microscopy and its applications in biomedicine and environmental monitoring.

    Science.gov (United States)

    Wu, Yichen; Ozcan, Aydogan

    2018-03-01

    Optical compound microscope has been a major tool in biomedical imaging for centuries. Its performance relies on relatively complicated, bulky and expensive lenses and alignment mechanics. In contrast, the lensless microscope digitally reconstructs microscopic images of specimens without using any lenses, as a result of which it can be made much smaller, lighter and lower-cost. Furthermore, the limited space-bandwidth product of objective lenses in a conventional microscope can be significantly surpassed by a lensless microscope. Such lensless imaging designs have enabled high-resolution and high-throughput imaging of specimens using compact, portable and cost-effective devices to potentially address various point-of-care, global-health and telemedicine related challenges. In this review, we discuss the operation principles and the methods behind lensless digital holographic on-chip microscopy. We also go over various applications that are enabled by cost-effective and compact implementations of lensless microscopy, including some recent work on air quality monitoring, which utilized machine learning for high-throughput and accurate quantification of particulate matter in air. Finally, we conclude with a brief future outlook of this computational imaging technology. Copyright © 2017 Elsevier Inc. All rights reserved.

  8. Lensless digital holographic microscopy and its applications in biomedicine and environmental monitoring

    KAUST Repository

    Wu, Yichen; Ozcan, Aydogan

    2017-01-01

    Optical compound microscope has been a major tool in biomedical imaging for centuries. Its performance relies on relatively complicated, bulky and expensive lenses and alignment mechanics. In contrast, the lensless microscope digitally reconstructs microscopic images of specimens without using any lenses, as a result of which it can be made much smaller, lighter and lower-cost. Furthermore, the limited space-bandwidth product of objective lenses in a conventional microscope can be significantly surpassed by a lensless microscope. Such lensless imaging designs have enabled high-resolution and high-throughput imaging of specimens using compact, portable and cost-effective devices to potentially address various point-of-care, global-health and telemedicine related challenges. In this review, we discuss the operation principles and the methods behind lensless digital holographic on-chip microscopy. We also go over various applications that are enabled by cost-effective and compact implementations of lensless microscopy, including some recent work on air quality monitoring, which utilized machine learning for high-throughput and accurate quantification of particulate matter in air. Finally, we conclude with a brief future outlook of this computational imaging technology.

  9. Lensless digital holographic microscopy and its applications in biomedicine and environmental monitoring

    KAUST Repository

    Wu, Yichen

    2017-08-31

    Optical compound microscope has been a major tool in biomedical imaging for centuries. Its performance relies on relatively complicated, bulky and expensive lenses and alignment mechanics. In contrast, the lensless microscope digitally reconstructs microscopic images of specimens without using any lenses, as a result of which it can be made much smaller, lighter and lower-cost. Furthermore, the limited space-bandwidth product of objective lenses in a conventional microscope can be significantly surpassed by a lensless microscope. Such lensless imaging designs have enabled high-resolution and high-throughput imaging of specimens using compact, portable and cost-effective devices to potentially address various point-of-care, global-health and telemedicine related challenges. In this review, we discuss the operation principles and the methods behind lensless digital holographic on-chip microscopy. We also go over various applications that are enabled by cost-effective and compact implementations of lensless microscopy, including some recent work on air quality monitoring, which utilized machine learning for high-throughput and accurate quantification of particulate matter in air. Finally, we conclude with a brief future outlook of this computational imaging technology.

  10. Polarization digital holographic microscopy using low-cost liquid crystal polarization rotators

    Science.gov (United States)

    Dovhaliuk, Rostyslav Yu

    2018-02-01

    Polarization imaging methods are actively used to study anisotropic objects. A number of methods and systems, such as imaging polarimeters, were proposed to measure the state of polarization of light that passed through the object. Digital holographic and interferometric approaches can be used to quantitatively measure both amplitude and phase of a wavefront. Using polarization modulation optics, the measurement capabilities of such interference-based systems can be extended to measure polarization-dependent parameters, such as phase retardation. Different kinds of polarization rotators can be used to alternate the polarization of a reference beam. Liquid crystals are used in a rapidly increasing number of different optoelectronic devices. Twisted nematic liquid crystals are widely used as amplitude modulators in electronic displays and light valves or shutter glass. Such devices are of particular interest for polarization imaging, as they can be used as polarization rotators, and due to large-scale manufacturing have relatively low cost. A simple Mach-Zehnder polarized holographic setup that uses modified shutter glass as a polarization rotator is demonstrated. The suggested approach is experimentally validated by measuring retardation of quarter-wave film.

  11. Bacteria interface interactions in Ecology-on-a-Chip by holographic microscopy and interferometry

    Science.gov (United States)

    Sheng, Jian; White, Andrew; Jalali, Maryam

    2017-11-01

    To improve our remediation of oil spills into marine system, one must understand the fate of oil under complex physical, chemical and biological environments. It is found that various processes such as wind, wave, turbulence and currents break oil into suspensions of droplets, in which states consumption by microbial further degrade the oil. Our prior studies show that marine bacteria do not adopt biofilm life style at oil-water interface in comparison to those near a solid substrate. On the contrary, Extracellular Polymer Substance of oily microbial aggregates is easily formed around an oil droplet. This highlights complexities of cell oil interactions at a liquid-liquid interface. To investigate these mechanisms at oil water interface quantitative, we have developed a micro-bioassay consisting of continuous microfluidics with a substrate printed with oil droplet array, namely Ecology-on-a-Chip, and an integrated digital holographic microscopy (DHM) and interferometer (DHI). The oil-water interface can be maintained over days (>10 days), suitable for conducting long-term observations. 3D movements of bacteria are tracked by DHM, while the interface morphology are measured by DHI at 10nm. The system is applied to Pseudomonas sp. (PS62) near crude-water interface and Escherichia coli (AW405) at hexadecane-water interface subject to low surface tension. The 3D motility, attachment, detachment and dispersion of cells as well as motility induced interface change are discussed. Funded by Gulf of Mexico Research Initiative (GoMRI).

  12. Digital holographic microscopy as a technique to monitor macrophages infected by leishmania

    Science.gov (United States)

    Mendoza-Rodríguez, E.; Organista-Castelblanco, C.; Camacho, M.; Monroy-Ramírez, F.

    2017-06-01

    The Digital Holographic Microscopy in Transmission technique (DHM) is considered a useful tool in the noninvasive quantifying of transparent biological objects like living cells. In this work, we propose this technique to study and to monitor control macrophages infected by Leishmania (mouse lineJ774.A1). When the promastigotes enter in contact with healthy macrophages, they got phagocytosed and latterly confined in the formed parasitophorous vacuole. These processes change the morphology and density of the host macrophage. Both parameters can be measured in a label-free analysis of cells with the aid of the DHM technique. Our technique begins with the optical record of the holograms using a modified Mach-Zehnder interferometer and the reconstruction of the complex optical field transmitted by macrophages. In the latter point, we employ the angular spectrum algorithm. With the complex optical field reconstruction, we compute the field amplitude and the phase difference maps, which leads to describe one morphological characterization for the samples. Using phase difference maps is possible to measure internal variations for the integral refractive index, estimating the infection level of macrophages. Through the changes in the integral refractive index, it is also possible to describe and quantify in two different states the evolution of the infection. With these results some parameters of cells have been quantified, making the DHM technique a viable tool for diagnosis of biological samples under the presence of some pathogen.

  13. Measurement of a 3D Ultrasonic Wavefield Using Pulsed Laser Holographic Microscopy for Ultrasonic Nondestructive Evaluation

    Directory of Open Access Journals (Sweden)

    Xing Wang

    2018-02-01

    Full Text Available In ultrasonic array imaging, 3D ultrasonic wavefields are normally recorded by an ultrasonic piezo array transducer. Its performance is limited by the configuration and size of the array transducer. In this paper, a method based on digital holographic interferometry is proposed to record the 3D ultrasonic wavefields instead of the array transducer, and the measurement system consisting of a pulsed laser, ultrasonic excitation, and synchronization and control circuit is designed. A consecutive sequence of holograms of ultrasonic wavefields are recorded by the system. The interferograms are calculated from the recorded holograms at different time sequence. The amplitudes and phases of the transient ultrasonic wavefields are recovered from the interferograms by phase unwrapping. The consecutive sequence of transient ultrasonic wavefields are stacked together to generate 3D ultrasonic wavefields. Simulation and experiments are carried out to verify the proposed technique, and preliminary results are presented.

  14. Giga-pixel lensfree holographic microscopy and tomography using color image sensors.

    Directory of Open Access Journals (Sweden)

    Serhan O Isikman

    Full Text Available We report Giga-pixel lensfree holographic microscopy and tomography using color sensor-arrays such as CMOS imagers that exhibit Bayer color filter patterns. Without physically removing these color filters coated on the sensor chip, we synthesize pixel super-resolved lensfree holograms, which are then reconstructed to achieve ~350 nm lateral resolution, corresponding to a numerical aperture of ~0.8, across a field-of-view of ~20.5 mm(2. This constitutes a digital image with ~0.7 Billion effective pixels in both amplitude and phase channels (i.e., ~1.4 Giga-pixels total. Furthermore, by changing the illumination angle (e.g., ± 50° and scanning a partially-coherent light source across two orthogonal axes, super-resolved images of the same specimen from different viewing angles are created, which are then digitally combined to synthesize tomographic images of the object. Using this dual-axis lensfree tomographic imager running on a color sensor-chip, we achieve a 3D spatial resolution of ~0.35 µm × 0.35 µm × ~2 µm, in x, y and z, respectively, creating an effective voxel size of ~0.03 µm(3 across a sample volume of ~5 mm(3, which is equivalent to >150 Billion voxels. We demonstrate the proof-of-concept of this lensfree optical tomographic microscopy platform on a color CMOS image sensor by creating tomograms of micro-particles as well as a wild-type C. elegans nematode.

  15. Smartphone-based quantitative measurements on holographic sensors.

    Science.gov (United States)

    Khalili Moghaddam, Gita; Lowe, Christopher Robin

    2017-01-01

    The research reported herein integrates a generic holographic sensor platform and a smartphone-based colour quantification algorithm in order to standardise and improve the determination of the concentration of analytes of interest. The utility of this approach has been exemplified by analysing the replay colour of the captured image of a holographic pH sensor in near real-time. Personalised image encryption followed by a wavelet-based image compression method were applied to secure the image transfer across a bandwidth-limited network to the cloud. The decrypted and decompressed image was processed through four principal steps: Recognition of the hologram in the image with a complex background using a template-based approach, conversion of device-dependent RGB values to device-independent CIEXYZ values using a polynomial model of the camera and computation of the CIEL*a*b* values, use of the colour coordinates of the captured image to segment the image, select the appropriate colour descriptors and, ultimately, locate the region of interest (ROI), i.e. the hologram in this case, and finally, application of a machine learning-based algorithm to correlate the colour coordinates of the ROI to the analyte concentration. Integrating holographic sensors and the colour image processing algorithm potentially offers a cost-effective platform for the remote monitoring of analytes in real time in readily accessible body fluids by minimally trained individuals.

  16. Smartphone-based quantitative measurements on holographic sensors.

    Directory of Open Access Journals (Sweden)

    Gita Khalili Moghaddam

    Full Text Available The research reported herein integrates a generic holographic sensor platform and a smartphone-based colour quantification algorithm in order to standardise and improve the determination of the concentration of analytes of interest. The utility of this approach has been exemplified by analysing the replay colour of the captured image of a holographic pH sensor in near real-time. Personalised image encryption followed by a wavelet-based image compression method were applied to secure the image transfer across a bandwidth-limited network to the cloud. The decrypted and decompressed image was processed through four principal steps: Recognition of the hologram in the image with a complex background using a template-based approach, conversion of device-dependent RGB values to device-independent CIEXYZ values using a polynomial model of the camera and computation of the CIEL*a*b* values, use of the colour coordinates of the captured image to segment the image, select the appropriate colour descriptors and, ultimately, locate the region of interest (ROI, i.e. the hologram in this case, and finally, application of a machine learning-based algorithm to correlate the colour coordinates of the ROI to the analyte concentration. Integrating holographic sensors and the colour image processing algorithm potentially offers a cost-effective platform for the remote monitoring of analytes in real time in readily accessible body fluids by minimally trained individuals.

  17. Phase-image-based content-addressable holographic data storage

    Science.gov (United States)

    John, Renu; Joseph, Joby; Singh, Kehar

    2004-03-01

    We propose and demonstrate the use of phase images for content-addressable holographic data storage. Use of binary phase-based data pages with 0 and π phase changes, produces uniform spectral distribution at the Fourier plane. The absence of strong DC component at the Fourier plane and more intensity of higher order spatial frequencies facilitate better recording of higher spatial frequencies, and improves the discrimination capability of the content-addressable memory. This improves the results of the associative recall in a holographic memory system, and can give low number of false hits even for small search arguments. The phase-modulated pixels also provide an opportunity of subtraction among data pixels leading to better discrimination between similar data pages.

  18. Simple and fast spectral domain algorithm for quantitative phase imaging of living cells with digital holographic microscopy

    Science.gov (United States)

    Min, Junwei; Yao, Baoli; Ketelhut, Steffi; Kemper, Björn

    2017-02-01

    The modular combination of optical microscopes with digital holographic microscopy (DHM) has been proven to be a powerful tool for quantitative live cell imaging. The introduction of condenser and different microscope objectives (MO) simplifies the usage of the technique and makes it easier to measure different kinds of specimens with different magnifications. However, the high flexibility of illumination and imaging also causes variable phase aberrations that need to be eliminated for high resolution quantitative phase imaging. The existent phase aberrations compensation methods either require add additional elements into the reference arm or need specimen free reference areas or separate reference holograms to build up suitable digital phase masks. These inherent requirements make them unpractical for usage with highly variable illumination and imaging systems and prevent on-line monitoring of living cells. In this paper, we present a simple numerical method for phase aberration compensation based on the analysis of holograms in spatial frequency domain with capabilities for on-line quantitative phase imaging. From a single shot off-axis hologram, the whole phase aberration can be eliminated automatically without numerical fitting or pre-knowledge of the setup. The capabilities and robustness for quantitative phase imaging of living cancer cells are demonstrated.

  19. Experimental teaching and training system based on volume holographic storage

    Science.gov (United States)

    Jiang, Zhuqing; Wang, Zhe; Sun, Chan; Cui, Yutong; Wan, Yuhong; Zou, Rufei

    2017-08-01

    The experiment of volume holographic storage for teaching and training the practical ability of senior students in Applied Physics is introduced. The students can learn to use advanced optoelectronic devices and the automatic control means via this experiment, and further understand the theoretical knowledge of optical information processing and photonics disciplines that have been studied in some courses. In the experiment, multiplexing holographic recording and readout is based on Bragg selectivity of volume holographic grating, in which Bragg diffraction angle is dependent on grating-recording angel. By using different interference angle between reference and object beams, the holograms can be recorded into photorefractive crystal, and then the object images can be read out from these holograms via angular addressing by using the original reference beam. In this system, the experimental data acquisition and the control of the optoelectronic devices, such as the shutter on-off, image loaded in SLM and image acquisition of a CCD sensor, are automatically realized by using LabVIEW programming.

  20. X-ray holographic microscopy using the atomic-force microscope

    International Nuclear Information System (INIS)

    Howells, M.R.; Jacobsen, C.J.; Lindaas, S.

    1993-09-01

    The present authors have been seeking for some time to improve the resolution of holographic microscopy and have engaged in a continuing series of experiments using the X1A soft x-ray undulator beam line at Brookhaven. The principle strategy for pushing the resolution lower in these experiments has been the use of polymer resists as x-ray detectors and the primary goal has been to develop the technique to become useful for examining wet biological material. In the present paper the authors report on progress in the use of resist for high-spatial-resolution x-ray detection. This is the key step in in-line holography and the one which sets the ultimate limit to the image resolution. The actual recording has always been quite easy, given a high-brightness undulator source, but the difficult step was the readout of the recorded pattern. The authors describe in what follows how they have built a special instrument: an atomic force microscope (AFM) to read holograms recorded in resist. They report the technical reasons for building, rather than buying, such an instrument and they give details of the design and performance of the device. The authors also describe the first attempts to use the system for real holography and the authors show results of both recorded holograms and the corresponding reconstructed images. Finally, the authors try to analyze the effect that these advances are likely to have on the future prospects for success in applications of x-ray holography and the degree to which the other technical systems that are needed for such success are available or within reach

  1. Holographic recording in thiophene-based polyester

    DEFF Research Database (Denmark)

    Matharu, Avtar Singh; Chambers-Asman, David; Jeeva, Shehzad

    2008-01-01

    The synthesis and optical data storage properties of a side-chain thiophene-phenyl azopolyester ThPhAzoP.ol is reported. The polyester is derived from diphenyl tetradecanedioate and a thiophenebased liquid crystalline diol which exhibits a short-lived enantiotropic SmA phase (Cryst 177.7 SmA 180.......4 I). The polyester ThPhAzoPol exhibits amorphous (Tg, 78.6 DC), crystalline and liquid crystalline character as evidenced by differential scanning calorimetry and thermal polarising microscopy. A grainy texture, which is thermally reversible, with increasing birefringence on cooling from...... the isotropic melt is observed. The polyester is amenable to optical data storage, showing efficient induced anisotropy, which is stable at room temperature. Polarisation gratings can be inscribed using orthogonally.linear and circularly polarised light to good effect (>10/0) and surface relief gratings...

  2. Modelling of a holographic interferometry based calorimeter for radiation dosimetry

    Science.gov (United States)

    Beigzadeh, A. M.; Vaziri, M. R. Rashidian; Ziaie, F.

    2017-08-01

    In this research work, a model for predicting the behaviour of holographic interferometry based calorimeters for radiation dosimetry is introduced. Using this technique for radiation dosimetry via measuring the variations of refractive index due to energy deposition of radiation has several considerable advantages such as extreme sensitivity and ability of working without normally used temperature sensors that disturb the radiation field. We have shown that the results of our model are in good agreement with the experiments performed by other researchers under the same conditions. This model also reveals that these types of calorimeters have the additional and considerable merits of transforming the dose distribution to a set of discernible interference fringes.

  3. Quantitative stain-free and continuous multimodal monitoring of wound healing in vitro with digital holographic microscopy.

    Directory of Open Access Journals (Sweden)

    Dominik Bettenworth

    Full Text Available Impaired epithelial wound healing has significant pathophysiological implications in several conditions including gastrointestinal ulcers, anastomotic leakage and venous or diabetic skin ulcers. Promising drug candidates for accelerating wound closure are commonly evaluated in in vitro wound assays. However, staining procedures and discontinuous monitoring are major drawbacks hampering accurate assessment of wound assays. We therefore investigated digital holographic microscopy (DHM to appropriately monitor wound healing in vitro and secondly, to provide multimodal quantitative information on morphological and functional cell alterations as well as on motility changes upon cytokine stimulation. Wound closure as reflected by proliferation and migration of Caco-2 cells in wound healing assays was studied and assessed in time-lapse series for 40 h in the presence of stimulating epidermal growth factor (EGF and inhibiting mitomycin c. Therefore, digital holograms were recorded continuously every thirty minutes. Morphological changes including cell thickness, dry mass and tissue density were analyzed by data from quantitative digital holographic phase microscopy. Stimulation of Caco-2 cells with EGF or mitomycin c resulted in significant morphological changes during wound healing compared to control cells. In conclusion, DHM allows accurate, stain-free and continuous multimodal quantitative monitoring of wound healing in vitro and could be a promising new technique for assessment of wound healing.

  4. CINCH (confocal incoherent correlation holography) super resolution fluorescence microscopy based upon FINCH (Fresnel incoherent correlation holography).

    Science.gov (United States)

    Siegel, Nisan; Storrie, Brian; Bruce, Marc; Brooker, Gary

    2015-02-07

    FINCH holographic fluorescence microscopy creates high resolution super-resolved images with enhanced depth of focus. The simple addition of a real-time Nipkow disk confocal image scanner in a conjugate plane of this incoherent holographic system is shown to reduce the depth of focus, and the combination of both techniques provides a simple way to enhance the axial resolution of FINCH in a combined method called "CINCH". An important feature of the combined system allows for the simultaneous real-time image capture of widefield and holographic images or confocal and confocal holographic images for ready comparison of each method on the exact same field of view. Additional GPU based complex deconvolution processing of the images further enhances resolution.

  5. Quantitative phase-digital holographic microscopy: a new imaging modality to identify original cellular biomarkers of diseases

    KAUST Repository

    Marquet, P.

    2016-05-03

    Quantitative phase microscopy (QPM) has recently emerged as a powerful label-free technique in the field of living cell imaging allowing to non-invasively measure with a nanometric axial sensitivity cell structure and dynamics. Since the phase retardation of a light wave when transmitted through the observed cells, namely the quantitative phase signal (QPS), is sensitive to both cellular thickness and intracellular refractive index related to the cellular content, its accurate analysis allows to derive various cell parameters and monitor specific cell processes, which are very likely to identify new cell biomarkers. Specifically, quantitative phase-digital holographic microscopy (QP-DHM), thanks to its numerical flexibility facilitating parallelization and automation processes, represents an appealing imaging modality to both identify original cellular biomarkers of diseases as well to explore the underlying pathophysiological processes.

  6. Digital holographic-based cancellable biometric for personal authentication

    International Nuclear Information System (INIS)

    Verma, Gaurav; Sinha, Aloka

    2016-01-01

    In this paper, we propose a new digital holographic-based cancellable biometric scheme for personal authentication and verification. The realization of cancellable biometric is presented by using an optoelectronic experimental approach, in which an optically recorded hologram of the fingerprint of a person is numerically reconstructed. Each reconstructed feature has its own perspective, which is utilized to generate user-specific fingerprint features by using a feature-extraction process. New representations of the user-specific fingerprint features can be obtained from the same hologram, by changing the reconstruction distance (d) by an amount Δd between the recording plane and the reconstruction plane. This parameter is the key to make the cancellable user-specific fingerprint features using a digital holographic technique, which allows us to choose different reconstruction distances when reissuing the user-specific fingerprint features in the event of compromise. We have shown theoretically that each user-specific fingerprint feature has a unique identity with a high discrimination ability, and the chances of a match between them are minimal. In this aspect, a recognition system has also been demonstrated using the fingerprint biometric of the enrolled person at a particular reconstruction distance. For the performance evaluation of a fingerprint recognition system—the false acceptance ratio, the false rejection ratio and the equal error rate are calculated using correlation. The obtained results show good discrimination ability between the genuine and the impostor populations with the highest recognition rate of 98.23%. (paper)

  7. Holographic images reconstructed from GMR-based fringe pattern

    Directory of Open Access Journals (Sweden)

    Kikuchi Hiroshi

    2013-01-01

    Full Text Available We have developed a magneto-optical spatial light modulator (MOSLM using giant magneto-resistance (GMR structures for realizing a holographic three-dimensional (3D display. For practical applications, reconstructed image of hologram consisting of GMR structures should be investigated in order to study the feasibility of the MOSLM. In this study, we fabricated a hologram with GMR based fringe-pattern and demonstrated a reconstructed image. A fringe-pattern convolving a crossshaped image was calculated by a conventional binary computer generated hologram (CGH technique. The CGH-pattern has 2,048 × 2,048 with 5 μm pixel pitch. The GMR stack consists of a Tb-Fe-Co/CoFe pinned layer, a Ag spacer, a Gd-Fe free layer for light modulation, and a Ru capping layer, was deposited by dc-magnetron sputtering. The GMR hologram was formed using photo-lithography and Krion milling processes, followed by the deposition of a Tb-Fe-Co reference layer with large coercivity and the same Kerr-rotation angle compared to the free layer, and a lift-off process. The reconstructed image of the ON-state was clearly observed and successfully distinguished from the OFF-state by switching the magnetization direction of the free-layer with an external magnetic field. These results indicate the possibility of realizing a holographic 3D display by the MOSLM using the GMR structures.

  8. Label-free sensor for automatic identification of erythrocytes using digital in-line holographic microscopy and machine learning.

    Science.gov (United States)

    Go, Taesik; Byeon, Hyeokjun; Lee, Sang Joon

    2018-04-30

    Cell types of erythrocytes should be identified because they are closely related to their functionality and viability. Conventional methods for classifying erythrocytes are time consuming and labor intensive. Therefore, an automatic and accurate erythrocyte classification system is indispensable in healthcare and biomedical fields. In this study, we proposed a new label-free sensor for automatic identification of erythrocyte cell types using a digital in-line holographic microscopy (DIHM) combined with machine learning algorithms. A total of 12 features, including information on intensity distributions, morphological descriptors, and optical focusing characteristics, is quantitatively obtained from numerically reconstructed holographic images. All individual features for discocytes, echinocytes, and spherocytes are statistically different. To improve the performance of cell type identification, we adopted several machine learning algorithms, such as decision tree model, support vector machine, linear discriminant classification, and k-nearest neighbor classification. With the aid of these machine learning algorithms, the extracted features are effectively utilized to distinguish erythrocytes. Among the four tested algorithms, the decision tree model exhibits the best identification performance for the training sets (n = 440, 98.18%) and test sets (n = 190, 97.37%). This proposed methodology, which smartly combined DIHM and machine learning, would be helpful for sensing abnormal erythrocytes and computer-aided diagnosis of hematological diseases in clinic. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. A new ball-on-disk vacuum tribometer with in situ measurement of the wear track by digital holographic microscopy

    Science.gov (United States)

    Meylan, B.; Ciani, D.; Zhang, B.; Cuche, E.; Wasmer, K.

    2017-12-01

    This contribution presents a new ball-on-disk vacuum tribometer with in situ measurement of the wear track by digital holographic microscopy. This new tribometer allows observation of the evolution of the wear track in situ and in real-time. The method combines a high vacuum high temperature ball-on-disk tribometer with a digital holographic microscope (DHM). The machine was tested and validated by taking DHM images during wear tests at room temperature and in vacuum at 2 · 10-6 of polished 100Cr6 steel disks. We demonstrated that the DHM system is well suited to monitor the evolution of the wear track during sliding. We found that, with an acquisition time of 0.1 ms for the DHM, the maximal linear speed is 10 cm s-1 to have reliable images. We proved, via scanning electron microscope (SEM) pictures, that the lines in the sliding direction in all DHM images exist. We also validated the new tribometer by having an excellent correlation between the images and profiles of the wear track taken by the DHM with the ones from a confocal microscope. Finally, the new tribometer combined with the DHM has four advantages. It can test under vacuum and various atmospheric conditions. The evolution of the wear track is measured in situ and in real-time. Hence, the problem of replacing the sample is avoided. Thanks to the DHM technology, the vertical accuracy of the topographical measurement is 4 nm.

  10. Reusable holographic velocimetry system based on polarization multiplexing in Bacteriorhodopsin

    NARCIS (Netherlands)

    Koek, W.D.; Chan, V.S.S.; Ooms, T.A.; Bhattacharya, N.; Westerweel, J.; Braat, J.J.M.

    2005-01-01

    We present a novel holographic particle image velocimetry (HPIV) system using a reversible holographic material as the recording medium. In HPIV the three-dimensional flow field throughout a volume is detected by adding small tracer particles to a normally transparent medium. By recording the

  11. Comparative analysis of the modified enclosed energy metric for self-focusing holograms from digital lensless holographic microscopy.

    Science.gov (United States)

    Trujillo, Carlos; Garcia-Sucerquia, Jorge

    2015-06-01

    A comparative analysis of the performance of the modified enclosed energy (MEE) method for self-focusing holograms recorded with digital lensless holographic microscopy is presented. Notwithstanding the MEE analysis previously published, no extended analysis of its performance has been reported. We have tested the MEE in terms of the minimum axial distance allowed between the set of reconstructed holograms to search for the focal plane and the elapsed time to obtain the focused image. These parameters have been compared with those for some of the already reported methods in the literature. The MEE achieves better results in terms of self-focusing quality but at a higher computational cost. Despite its longer processing time, the method remains within a time frame to be technologically attractive. Modeled and experimental holograms have been utilized in this work to perform the comparative study.

  12. Investigations on the change of texture of plant cells due to preservative treatments by digital holographic microscopy

    Science.gov (United States)

    Vora, Priyanka; Anand, Arun

    2014-10-01

    Texture change is observed in preserved fruits and vegetables. Responsible factors for texture change during preservative treatments are cell morphology, cell wall structure, cell turger, water content and some biochemical components, and also the environmental conditions. Digital Holographic microscopy (DHM) is a quantitative phase contrast imaging technique, which provides three dimensional optical thickness profiles of transparent specimen. Using DHM the morphology of plant cells preserved by refrigeration or stored in vinegar or in sodium chloride can be obtained. This information about the spatio-temporal evolution of optical volume and thickness can be an important tool in area of food processing. Also from the three dimensional images, the texture of the cell can be retrieved and can be investigated under varying conditions.

  13. Model-based magnetization retrieval from holographic phase images

    Energy Technology Data Exchange (ETDEWEB)

    Röder, Falk, E-mail: f.roeder@hzdr.de [Helmholtz-Zentrum Dresden-Rossendorf, Institut für Ionenstrahlphysik und Materialforschung, Bautzner Landstr. 400, D-01328 Dresden (Germany); Triebenberg Labor, Institut für Strukturphysik, Technische Universität Dresden, D-01062 Dresden (Germany); Vogel, Karin [Triebenberg Labor, Institut für Strukturphysik, Technische Universität Dresden, D-01062 Dresden (Germany); Wolf, Daniel [Helmholtz-Zentrum Dresden-Rossendorf, Institut für Ionenstrahlphysik und Materialforschung, Bautzner Landstr. 400, D-01328 Dresden (Germany); Triebenberg Labor, Institut für Strukturphysik, Technische Universität Dresden, D-01062 Dresden (Germany); Hellwig, Olav [Helmholtz-Zentrum Dresden-Rossendorf, Institut für Ionenstrahlphysik und Materialforschung, Bautzner Landstr. 400, D-01328 Dresden (Germany); AG Magnetische Funktionsmaterialien, Institut für Physik, Technische Universität Chemnitz, D-09126 Chemnitz (Germany); HGST, A Western Digital Company, 3403 Yerba Buena Rd., San Jose, CA 95135 (United States); Wee, Sung Hun [HGST, A Western Digital Company, 3403 Yerba Buena Rd., San Jose, CA 95135 (United States); Wicht, Sebastian; Rellinghaus, Bernd [IFW Dresden, Institute for Metallic Materials, P.O. Box 270116, D-01171 Dresden (Germany)

    2017-05-15

    The phase shift of the electron wave is a useful measure for the projected magnetic flux density of magnetic objects at the nanometer scale. More important for materials science, however, is the knowledge about the magnetization in a magnetic nano-structure. As demonstrated here, a dominating presence of stray fields prohibits a direct interpretation of the phase in terms of magnetization modulus and direction. We therefore present a model-based approach for retrieving the magnetization by considering the projected shape of the nano-structure and assuming a homogeneous magnetization therein. We apply this method to FePt nano-islands epitaxially grown on a SrTiO{sub 3} substrate, which indicates an inclination of their magnetization direction relative to the structural easy magnetic [001] axis. By means of this real-world example, we discuss prospects and limits of this approach. - Highlights: • Retrieval of the magnetization from holographic phase images. • Magnetostatic model constructed for a magnetic nano-structure. • Decomposition into homogeneously magnetized components. • Discretization of a each component by elementary cuboids. • Analytic solution for the phase of a magnetized cuboid considered. • Fitting a set of magnetization vectors to experimental phase images.

  14. Ultrafast layer based computer-generated hologram calculation with sparse template holographic fringe pattern for 3-D object.

    Science.gov (United States)

    Kim, Hak Gu; Man Ro, Yong

    2017-11-27

    In this paper, we propose a new ultrafast layer based CGH calculation that exploits the sparsity of hologram fringe pattern in 3-D object layer. Specifically, we devise a sparse template holographic fringe pattern. The holographic fringe pattern on a depth layer can be rapidly calculated by adding the sparse template holographic fringe patterns at each object point position. Since the size of sparse template holographic fringe pattern is much smaller than that of the CGH plane, the computational load can be significantly reduced. Experimental results show that the proposed method achieves 10-20 msec for 1024x1024 pixels providing visually plausible results.

  15. Label free imaging of cell-substrate contacts by holographic total internal reflection microscopy.

    Science.gov (United States)

    Mandracchia, Biagio; Gennari, Oriella; Marchesano, Valentina; Paturzo, Melania; Ferraro, Pietro

    2017-09-01

    The study of cell adhesion contacts is pivotal to understand cell mechanics and interaction at substrates or chemical and physical stimuli. We designed and built a HoloTIR microscope for label-free quantitative phase imaging of total internal reflection. Here we show for the first time that HoloTIR is a good choice for label-free study of focal contacts and of cell/substrate interaction as its sensitivity is enhanced in comparison with standard TIR microscopy. Finally, the simplicity of implementation and relative low cost, due to the requirement of less optical components, make HoloTIR a reasonable alternative, or even an addition, to TIRF microscopy for mapping cell/substratum topography. As a proof of concept, we studied the formation of focal contacts of fibroblasts on three substrates with different levels of affinity for cell adhesion. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  16. Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy.

    Science.gov (United States)

    Rappaz, Benjamin; Cano, Elena; Colomb, Tristan; Kühn, Jonas; Depeursinge, Christian; Simanis, Viesturs; Magistretti, Pierre J; Marquet, Pierre

    2009-01-01

    Digital holography microscopy (DHM) is an optical technique which provides phase images yielding quantitative information about cell structure and cellular dynamics. Furthermore, the quantitative phase images allow the derivation of other parameters, including dry mass production, density, and spatial distribution. We have applied DHM to study the dry mass production rate and the dry mass surface density in wild-type and mutant fission yeast cells. Our study demonstrates the applicability of DHM as a tool for label-free quantitative analysis of the cell cycle and opens the possibility for its use in high-throughput screening.

  17. Holographic memory system based on projection recording of computer-generated 1D Fourier holograms.

    Science.gov (United States)

    Betin, A Yu; Bobrinev, V I; Donchenko, S S; Odinokov, S B; Evtikhiev, N N; Starikov, R S; Starikov, S N; Zlokazov, E Yu

    2014-10-01

    Utilization of computer generation of holographic structures significantly simplifies the optical scheme that is used to record the microholograms in a holographic memory record system. Also digital holographic synthesis allows to account the nonlinear errors of the record system to improve the microholograms quality. The multiplexed record of holograms is a widespread technique to increase the data record density. In this article we represent the holographic memory system based on digital synthesis of amplitude one-dimensional (1D) Fourier transform holograms and the multiplexed record of these holograms onto the holographic carrier using optical projection scheme. 1D Fourier transform holograms are very sensitive to orientation of the anamorphic optical element (cylindrical lens) that is required for encoded data object reconstruction. The multiplex record of several holograms with different orientation in an optical projection scheme allowed reconstruction of the data object from each hologram by rotating the cylindrical lens on the corresponding angle. Also, we discuss two optical schemes for the recorded holograms readout: a full-page readout system and line-by-line readout system. We consider the benefits of both systems and present the results of experimental modeling of 1D Fourier holograms nonmultiplex and multiplex record and reconstruction.

  18. High-throughput characterization of stresses in thin film materials libraries using Si cantilever array wafers and digital holographic microscopy.

    Science.gov (United States)

    Lai, Y W; Hamann, S; Ehmann, M; Ludwig, A

    2011-06-01

    We report the development of an advanced high-throughput stress characterization method for thin film materials libraries sputter-deposited on micro-machined cantilever arrays consisting of around 1500 cantilevers on 4-inch silicon-on-insulator wafers. A low-cost custom-designed digital holographic microscope (DHM) is employed to simultaneously monitor the thin film thickness, the surface topography and the curvature of each of the cantilevers before and after deposition. The variation in stress state across the thin film materials library is then calculated by Stoney's equation based on the obtained radii of curvature of the cantilevers and film thicknesses. DHM with nanometer-scale out-of-plane resolution allows stress measurements in a wide range, at least from several MPa to several GPa. By using an automatic x-y translation stage, the local stresses within a 4-inch materials library are mapped with high accuracy within 10 min. The speed of measurement is greatly improved compared with the prior laser scanning approach that needs more than an hour of measuring time. A high-throughput stress measurement of an as-deposited Fe-Pd-W materials library was evaluated for demonstration. The fast characterization method is expected to accelerate the development of (functional) thin films, e.g., (magnetic) shape memory materials, whose functionality is greatly stress dependent. © 2011 American Institute of Physics

  19. High-throughput characterization of stresses in thin film materials libraries using Si cantilever array wafers and digital holographic microscopy

    International Nuclear Information System (INIS)

    Lai, Y. W.; Ludwig, A.; Hamann, S.; Ehmann, M.

    2011-01-01

    We report the development of an advanced high-throughput stress characterization method for thin film materials libraries sputter-deposited on micro-machined cantilever arrays consisting of around 1500 cantilevers on 4-inch silicon-on-insulator wafers. A low-cost custom-designed digital holographic microscope (DHM) is employed to simultaneously monitor the thin film thickness, the surface topography and the curvature of each of the cantilevers before and after deposition. The variation in stress state across the thin film materials library is then calculated by Stoney's equation based on the obtained radii of curvature of the cantilevers and film thicknesses. DHM with nanometer-scale out-of-plane resolution allows stress measurements in a wide range, at least from several MPa to several GPa. By using an automatic x-y translation stage, the local stresses within a 4-inch materials library are mapped with high accuracy within 10 min. The speed of measurement is greatly improved compared with the prior laser scanning approach that needs more than an hour of measuring time. A high-throughput stress measurement of an as-deposited Fe-Pd-W materials library was evaluated for demonstration. The fast characterization method is expected to accelerate the development of (functional) thin films, e.g., (magnetic) shape memory materials, whose functionality is greatly stress dependent.

  20. Review of quantitative phase-digital holographic microscopy: promising novel imaging technique to resolve neuronal network activity and identify cellular biomarkers of psychiatric disorders

    KAUST Repository

    Marquet, Pierre

    2014-09-22

    Quantitative phase microscopy (QPM) has recently emerged as a new powerful quantitative imaging technique well suited to noninvasively explore a transparent specimen with a nanometric axial sensitivity. In this review, we expose the recent developments of quantitative phase-digital holographic microscopy (QP-DHM). Quantitative phase-digital holographic microscopy (QP-DHM) represents an important and efficient quantitative phase method to explore cell structure and dynamics. In a second part, the most relevant QPM applications in the field of cell biology are summarized. A particular emphasis is placed on the original biological information, which can be derived from the quantitative phase signal. In a third part, recent applications obtained, with QP-DHM in the field of cellular neuroscience, namely the possibility to optically resolve neuronal network activity and spine dynamics, are presented. Furthermore, potential applications of QPM related to psychiatry through the identification of new and original cell biomarkers that, when combined with a range of other biomarkers, could significantly contribute to the determination of high risk developmental trajectories for psychiatric disorders, are discussed.

  1. Measuring opto-thermal parameters of basalt fibers using digital holographic microscopy.

    Science.gov (United States)

    Yassien, Khaled M; Agour, Mostafa

    2017-02-01

    A method for studying the effect of temperature on the optical properties of basalt fiber is presented. It is based on recording a set of phase-shifted digital holograms for the sample under the test. The holograms are obtained utilizing a system based on Mach-Zehnder interferometer, where the fiber sample inserted in an immersion liquid is placed within a temperature controlled chamber. From the recorded digital holograms the optical path differences which are used to calculate the refractive indices are determined. The accuracy in the measurement of refractive indices is in the range of 4 × 10 -4 . The influence of temperature on the dispersion parameters, polarizability per unit volume and dielectric susceptibility are also obtained. Moreover, the values of dispersion and oscillation energies and Cauchy's constants are provided at different temperatures. © 2016 Wiley Periodicals, Inc.

  2. An information entropy model on clinical assessment of patients based on the holographic field of meridian

    Science.gov (United States)

    Wu, Jingjing; Wu, Xinming; Li, Pengfei; Li, Nan; Mao, Xiaomei; Chai, Lihe

    2017-04-01

    Meridian system is not only the basis of traditional Chinese medicine (TCM) method (e.g. acupuncture, massage), but also the core of TCM's basic theory. This paper has introduced a new informational perspective to understand the reality and the holographic field of meridian. Based on maximum information entropy principle (MIEP), a dynamic equation for the holographic field has been deduced, which reflects the evolutionary characteristics of meridian. By using self-organizing artificial neural network as algorithm, the evolutionary dynamic equation of the holographic field can be resolved to assess properties of meridians and clinically diagnose the health characteristics of patients. Finally, through some cases from clinical patients (e.g. a 30-year-old male patient, an apoplectic patient, an epilepsy patient), we use this model to assess the evolutionary properties of meridians. It is proved that this model not only has significant implications in revealing the essence of meridian in TCM, but also may play a guiding role in clinical assessment of patients based on the holographic field of meridians.

  3. Accurate reconstruction in digital holographic microscopy using Fresnel dual-tree complex wavelet transform

    Science.gov (United States)

    Zhang, Xiaolei; Zhang, Xiangchao; Yuan, He; Zhang, Hao; Xu, Min

    2018-02-01

    Digital holography is a promising measurement method in the fields of bio-medicine and micro-electronics. But the captured images of digital holography are severely polluted by the speckle noise because of optical scattering and diffraction. Via analyzing the properties of Fresnel diffraction and the topographies of micro-structures, a novel reconstruction method based on the dual-tree complex wavelet transform (DT-CWT) is proposed. This algorithm is shiftinvariant and capable of obtaining sparse representations for the diffracted signals of salient features, thus it is well suited for multiresolution processing of the interferometric holograms of directional morphologies. An explicit representation of orthogonal Fresnel DT-CWT bases and a specific filtering method are developed. This method can effectively remove the speckle noise without destroying the salient features. Finally, the proposed reconstruction method is compared with the conventional Fresnel diffraction integration and Fresnel wavelet transform with compressive sensing methods to validate its remarkable superiority on the aspects of topography reconstruction and speckle removal.

  4. Imaging-in-flow: digital holographic microscopy as a novel tool to detect and classify nanoplanktonic organisms

    NARCIS (Netherlands)

    Zetsche, E.-M.; El Mallahi, A.; Dubois, F.; Yourassowsky, C.; Kromkamp, J.C.; Meysman, F.J.R.

    2014-01-01

    Traditional taxonomic identification of planktonic organisms is based on light microscopy, which is both time-consuming and tedious. In response, novel ways of automated (machine) identification, such as flow cytometry, have been investigated over the last two decades. To improve the taxonomic

  5. Quantitative investigation of red blood cell three-dimensional geometric and chemical changes in the storage lesion using digital holographic microscopy.

    Science.gov (United States)

    Jaferzadeh, Keyvan; Moon, Inkyu

    2015-11-01

    Quantitative phase information obtained by digital holographic microscopy (DHM) can provide new insight into the functions and morphology of single red blood cells (RBCs). Since the functionality of a RBC is related to its three-dimensional (3-D) shape, quantitative 3-D geometric changes induced by storage time can help hematologists realize its optimal functionality period. We quantitatively investigate RBC 3-D geometric changes in the storage lesion using DHM. Our experimental results show that the substantial geometric transformation of the biconcave-shaped RBCs to the spherocyte occurs due to RBC storage lesion. This transformation leads to progressive loss of cell surface area, surface-to-volume ratio, and functionality of RBCs. Furthermore, our quantitative analysis shows that there are significant correlations between chemical and morphological properties of RBCs.

  6. Holographic anyonic superfluidity

    Science.gov (United States)

    Jokela, Niko; Lifschytz, Gilad; Lippert, Matthew

    2013-10-01

    Starting with a holographic construction for a fractional quantum Hall state based on the D3-D7' system, we explore alternative quantization conditions for the bulk gauge fields. This gives a description of a quantum Hall state with various filling fractions. For a particular alternative quantization of the bulk gauge fields, we obtain a holographic anyon fluid in a vanishing background magnetic field. We show that this system is a superfluid, exhibiting the relevant gapless excitation.

  7. Holographic memories

    DEFF Research Database (Denmark)

    Ramanujam, P.S.; Berg, R.H.; Hvilsted, Søren

    1999-01-01

    A Two-dimensional holographic memory for archival storage is described. Assuming a coherent transfer function, an A4 page can be stored at high resolution in an area of 1 mm(2). Recently developed side-chain liquid crystalline azobenzene polyesters are found to be suitable media for holographic...

  8. Three-dimensional motion-picture imaging of dynamic object by parallel-phase-shifting digital holographic microscopy using an inverted magnification optical system

    Science.gov (United States)

    Fukuda, Takahito; Shinomura, Masato; Xia, Peng; Awatsuji, Yasuhiro; Nishio, Kenzo; Matoba, Osamu

    2017-04-01

    We constructed a parallel-phase-shifting digital holographic microscopy (PPSDHM) system using an inverted magnification optical system, and succeeded in three-dimensional (3D) motion-picture imaging for 3D displacement of a microscopic object. In the PPSDHM system, the inverted and afocal magnification optical system consisted of a microscope objective (16.56 mm focal length and 0.25 numerical aperture) and a convex lens (300 mm focal length and 82 mm aperture diameter). A polarization-imaging camera was used to record multiple phase-shifted holograms with a single-shot exposure. We recorded an alum crystal, sinking down in aqueous solution of alum, by the constructed PPSDHM system at 60 frames/s for about 20 s and reconstructed high-quality 3D motion-picture image of the crystal. Then, we calculated amounts of displacement of the crystal from the amounts in the focus plane and the magnifications of the magnification optical system, and obtained the 3D trajectory of the crystal by that amounts.

  9. Coherence-controlled holographic microscopy enabled recognition of necrosis as the mechanism of cancer cells death after exposure to cytopathic turbid emulsion

    Science.gov (United States)

    Collakova, Jana; Krizova, Aneta; Kollarova, Vera; Dostal, Zbynek; Slaba, Michala; Vesely, Pavel; Chmelik, Radim

    2015-11-01

    Coherence-controlled holographic microscopy (CCHM) in low-coherence mode possesses a pronounced coherence gate effect. This offers an option to investigate the details of cellular events leading to cell death caused by cytopathic turbid emulsions. CCHM capacity was first assessed in model situations that showed clear images obtained with low coherence of illumination but not with high coherence of illumination. Then, the form of death of human cancer cells induced by treatment with biologically active phospholipids (BAPs) preparation was investigated. The observed overall retraction of cell colony was apparently caused by the release of cell-to-substratum contacts. This was followed by the accumulation of granules decorating the nuclear membrane. Then, the occurrence of nuclear membrane indentations signaled the start of damage to the integrity of the cell nucleus. In the final stage, cells shrunk and disintegrated. This indicated that BAPs cause cell death by necrosis and not apoptosis. An intriguing option of checking the fate of cancer cells caused by the anticipated cooperative effect after adding another tested substance sodium dichloroacetate to turbid emulsion is discussed on grounds of pilot experiments. Such observations should reveal the impact and mechanism of action of the interacting drugs on cell behavior and fate that would otherwise remain hidden in turbid milieu.

  10. Handheld Fluorescence Microscopy based Flow Analyzer.

    Science.gov (United States)

    Saxena, Manish; Jayakumar, Nitin; Gorthi, Sai Siva

    2016-03-01

    Fluorescence microscopy has the intrinsic advantages of favourable contrast characteristics and high degree of specificity. Consequently, it has been a mainstay in modern biological inquiry and clinical diagnostics. Despite its reliable nature, fluorescence based clinical microscopy and diagnostics is a manual, labour intensive and time consuming procedure. The article outlines a cost-effective, high throughput alternative to conventional fluorescence imaging techniques. With system level integration of custom-designed microfluidics and optics, we demonstrate fluorescence microscopy based imaging flow analyzer. Using this system we have imaged more than 2900 FITC labeled fluorescent beads per minute. This demonstrates high-throughput characteristics of our flow analyzer in comparison to conventional fluorescence microscopy. The issue of motion blur at high flow rates limits the achievable throughput in image based flow analyzers. Here we address the issue by computationally deblurring the images and show that this restores the morphological features otherwise affected by motion blur. By further optimizing concentration of the sample solution and flow speeds, along with imaging multiple channels simultaneously, the system is capable of providing throughput of about 480 beads per second.

  11. Holographic interferometric and correlation-based laser speckle metrology for 3D deformations in dentistry

    Science.gov (United States)

    Dekiff, Markus; Kemper, Björn; Kröger, Elke; Denz, Cornelia; Dirksen, Dieter

    2017-03-01

    The mechanical loading of dental restorations and hard tissue is often investigated numerically. For validation and optimization of such simulations, comparisons with measured deformations are essential. We combine digital holographic interferometry and digital speckle photography for the determination of microscopic deformations with a photogrammetric method that is based on digital image correlation of a projected laser speckle pattern. This multimodal workstation allows the simultaneous acquisition of the specimen's macroscopic 3D shape and thus a quantitative comparison of measured deformations with simulation data. In order to demonstrate the feasibility of our system, two applications are presented: the quantitative determination of (1) the deformation of a mandible model due to mechanical loading of an inserted dental implant and of (2) the deformation of a (dental) bridge model under mechanical loading. The results were compared with data from finite element analyses of the investigated applications. The experimental results showed close agreement with those of the simulations.

  12. The human CFTR protein expressed in CHO cells activates aquaporin-3 in a cAMP-dependent pathway: study by digital holographic microscopy

    KAUST Repository

    Jourdain, P.

    2013-12-11

    The transmembrane water movements during cellular processes and their relationship to ionic channel activity remain largely unknown. As an example, in epithelial cells it was proposed that the movement of water could be directly linked to cystic fibrosis transmembrane conductance regulator (CFTR) protein activity through a cAMP-stimulated aqueous pore, or be dependent on aquaporin. Here, we used digital holographic microscopy (DHM) an interferometric technique to quantify in situ the transmembrane water fluxes during the activity of the epithelial chloride channel, CFTR, measured by patch-clamp and iodide efflux techniques. We showed that the water transport measured by DHM is fully inhibited by the selective CFTR blocker CFTRinh172 and is absent in cells lacking CFTR. Of note, in cells expressing the mutated version of CFTR (F508del-CFTR), which mimics the most common genetic alteration encountered in cystic fibrosis, we also show that the water movement is profoundly altered but restored by pharmacological manipulation of F508del-CFTR-defective trafficking. Importantly, whereas activation of this endogenous water channel required a cAMP-dependent stimulation of CFTR, activation of CFTR or F508del-CFTR by two cAMP-independent CFTR activators, genistein and MPB91, failed to trigger water movements. Finally, using a specific small-interfering RNA against the endogenous aquaporin AQP3, the water transport accompanying CFTR activity decreased. We conclude that water fluxes accompanying CFTR activity are linked to AQP3 but not to a cAMP-stimulated aqueous pore in the CFTR protein.

  13. Holographic View of the Brain Memory Mechanism Based on Evanescent Superluminal Photons

    Directory of Open Access Journals (Sweden)

    Takaaki Musha

    2012-08-01

    Full Text Available D. Pollen and M. Trachtenberg proposed the holographic brain theory to help explain the existence of photographic memories in some people. They suggested that such individuals had more vivid memories because they somehow could access a very large region of their memory holograms. Hameroff suggested in his paper that cylindrical neuronal microtubule cavities, or centrioles, function as waveguides for the evanescent photons for quantum signal processing. The supposition is that microtubular structures of the brain function as a coherent fiber bundle set used to store holographic images, as would a fiber-optic holographic system. In this paper, the author proposes that superluminal photons propagating inside the microtubules via evanescent waves could provide the access needed to record or retrieve a quantum coherent entangled holographic memory.

  14. Optical Security System Based on the Biometrics Using Holographic Storage Technique with a Simple Data Format

    Science.gov (United States)

    Jun, An Won

    2006-01-01

    We implement a first practical holographic security system using electrical biometrics that combines optical encryption and digital holographic memory technologies. Optical information for identification includes a picture of face, a name, and a fingerprint, which has been spatially multiplexed by random phase mask used for a decryption key. For decryption in our biometric security system, a bit-error-detection method that compares the digital bit of live fingerprint with of fingerprint information extracted from hologram is used.

  15. Real-time Holographic Display Based on a Super Fast Response Thin Film

    International Nuclear Information System (INIS)

    Gao, Hongyue; Li, Xiao; He, Zhenghong; Su, Yikai; Poon, Ting-Chung

    2013-01-01

    Real-time dynamic holographic display is obtained with super fast response in a thin film without any applied electric field. Holograms can be refreshed in the order of a millisecond and there is no cross talk between the recorded holograms because the hologram formed in the film is transient and can be completely self erased, and the hologram formation time and self-erasure time are both ∼1 ms. Holographic video display is achieved, which shows the real-time holographic image display capability of the thin film, and its much higher resolution than those of commercially available spatial light modulators. Furthermore, multiplexed hologram display using two polarization directions of a recorded light and multiple color holographic display at different laser wavelengths are presented, which demonstrate the feasibility of a RGB color holographic three-dimensional display with the thin film. Because the sample is easy to be fabricated into a large size screen and needs no external applied electric field, we think that the film can be developed into a large-size, dynamic, and color holographic three-dimensional display in the future.

  16. Digital holographic inspection for drying processes of paint films and ink dots

    Science.gov (United States)

    Yokota, M.; Aoyama, F.

    2017-06-01

    Digital holographic techniques to investigate drying processes of both paint films and ink dot is presented. The proposed technique based on digital holographic interferometry can achieve both visualization of variations and analysis of dryness of paint films in the drying process by using phase changes between two subsequent reconstructed complex amplitudes of the reflected light from the film. To follow the drying processes, holograms are recorded at a constant time interval. Phase-shifting digital holography has been applied to analyze the dryness of commercial paints applied on the metal plate. For analysis of an ink dot having diameter of a few hundred micrometers, digital holographic microscopy is applied to evaluating the time history of dryness of ink dot in the drying process. This paper describes these holographic techniques applied to the commercially available paint and ink and presents some experimental results.

  17. Atomic Force Microscopy Based Cell Shape Index

    Science.gov (United States)

    Adia-Nimuwa, Usienemfon; Mujdat Tiryaki, Volkan; Hartz, Steven; Xie, Kan; Ayres, Virginia

    2013-03-01

    Stellation is a measure of cell physiology and pathology for several cell groups including neural, liver and pancreatic cells. In the present work, we compare the results of a conventional two-dimensional shape index study of both atomic force microscopy (AFM) and fluorescent microscopy images with the results obtained using a new three-dimensional AFM-based shape index similar to sphericity index. The stellation of astrocytes is investigated on nanofibrillar scaffolds composed of electrospun polyamide nanofibers that has demonstrated promise for central nervous system (CNS) repair. Recent work by our group has given us the ability to clearly segment the cells from nanofibrillar scaffolds in AFM images. The clear-featured AFM images indicated that the astrocyte processes were longer than previously identified at 24h. It was furthermore shown that cell spreading could vary significantly as a function of environmental parameters, and that AFM images could record these variations. The new three-dimensional AFM-based shape index incorporates the new information: longer stellate processes and cell spreading. The support of NSF PHY-095776 is acknowledged.

  18. Holographic Microscopy: A Feasibility Experiment

    Science.gov (United States)

    McCann, Guy W.

    The department of Physics of the University of Glasgow was concerned about losing students after the end of the level 1 Physics course. The current research project started as an attempt to find out the reasons for this, but moved to investigate attitudes towards Physics at several stages during secondary school and attitudes towards science with primary pupils. Analyses of factors, which influence students' intentions towards studying Physics, were performed against the background of the Theory of Planned Behaviour, which interprets people's behaviour by considering three factors: attitude towards behaviour (advantages or disadvantages of being involved in the behaviour, e.g. studying Physics for Honours); subjective norm (approval or disapproval of important people towards engaging in the behaviour, e.g. parents, teacher, general norms of the society); perceived behavioural control (skills, knowledge, cooperation of others, abilities, efforts required to perform the behaviour). Analysis of these factors revealed some reasons for students' withdrawal from Physics after level 1 and pointed to factors which may facilitate students' persistence in the subject. A general analysis of level 1 and level 2 students' attitudes towards different aspects of the university Physics course revealed that the level 1 students' attitudes towards their university course of lectures and course of laboratories tended to be negatively polarised. Recommendations were suggested on the basis of the gathered evidence about how to make students' experience in university Physics more satisfactory for them. The data obtained from the separate analyses of females' and males' attitudes towards university Physics course have showed that attitudes of females and males were similar. The only significant difference between level 1 females and males was found to be the perceived behavioural control factor (students' attitudes towards course difficulty, attitudes towards work load in the course), which was significantly lower for females than for males. Special attention in this work was given to the problem of university Physics laboratory practice. Possibilities to improve students' attitudes towards laboratory work were discussed. This could be done through introduction of pre-lab (aimed to consolidate students' grasp of the necessary background for performing the experiment) and post-lab (aimed to provide students with opportunity to apply the theory they have learned and skills they have obtained from doing laboratory work to solve everyday problems). Examples of pre- and post-labs that were designed for the first term of the level 1 university Physics laboratory practice are given in the Appendix T. The project was extended from the university to the school area where cross-age analyses (measurements at one time with pupils of different age) of pupils' attitudes towards Science/Physics lessons were performed. Pupils from upper Primary P6/P7 up to Higher S5/S6 were involved in the research. These analyses have shown that patterns of Scottish pupils' attitudes towards Science/Physics lessons are not linear with age: attitudes of pupils who were self-selected towards the subject were not always more positive than attitudes of lower level pupils: primary school pupils' attitudes towards science lessons were significantly more positive than attitudes of secondary S2 pupils; pupils doing Standard Grade Physics course were similar in their evaluations of Physics lessons at both S3 and S4 levels; at Higher Grade Physics pupils' attitudes towards science lessons were significantly less positive than attitudes of Standard Grade Physics pupils. Pupils' attitudes towards Science/Physics lessons can be considered as a good indicator of pupils' reactions towards existing syllabuses in Science and Physics. Special attention in this study was devoted to the so-called "problem of girls in Physics". Separate analyses of boys' and girls' interests towards Physics topics revealed that although boys and girls are equally interested in certain areas of the subject, there are areas in Physics where boys and girls interests are significantly different. No differences were found in intensity of boys' and girls' interests towards suggested Physics topics at primary P6/P7 level, S3 and S5/S6 levels. At S2 and S4 levels a significant decline of girls' interests relative to boys interests was observed. S2 and S4 stages are decision making ones when pupils have the opportunity to select courses for the future. It was also revealed that the ratio of boys to girls in Physics once established at S2 level remains unchanged through the years of Standard Grade and Higher Grade Physics courses. This may indicate that if the number of girls in Physics is an issue for concern then attention should be paid to the primary and, especially early secondary years to attract girls to Physics. School Physics courses in Scotland revealed a high retention rate of girls in Physics. Analyses of preferred activities revealed that practical work is the most enjoyable activity in Science/Physics lessons for both girls and boys at every stage of schooling and studying the theory was found to be the least enjoyable activity at school for both genders at every age. The picture was almost the reverse with university Physics students.

  19. Optical studies in the holographic ground station

    Science.gov (United States)

    Workman, Gary L.

    1991-01-01

    The Holographic Group System (HGS) Facility in rooms 22 & 123, Building 4708 has been developed to provide for ground based research in determining pre-flight parameters and analyzing the results from space experiments. The University of Alabama, Huntsville (UAH) has researched the analysis aspects of the HGS and reports their findings here. Some of the results presented here also occur in the Facility Operating Procedure (FOP), which contains instructions for power up, operation, and powerdown of the Fluid Experiment System (FES) Holographic Ground System (HGS) Test Facility for the purpose of optically recording fluid and/or crystal behavior in a test article during ground based testing through the construction of holograms and recording of videotape. The alignment of the optical bench components, holographic reconstruction and and microscopy alignment sections were also included in the document for continuity even though they are not used until after optical recording of the test article) setup of support subsystems and the Automated Holography System (AHS) computer. The HGS provides optical recording and monitoring during GCEL runs or development testing of potential FES flight hardware or software. This recording/monitoring can be via 70mm holographic film, standard videotape, or digitized images on computer disk. All optical bench functions necessary to construct holograms will be under the control of the AHS personal computer (PC). These include type of exposure, time intervals between exposures, exposure length, film frame identification, film advancement, film platen evacuation and repressurization, light source diffuser introduction, and control of realtime video monitoring. The completed sequence of hologram types (single exposure, diffuse double exposure, etc.) and their time of occurrence can be displayed, printed, or stored on floppy disk posttest for the user.

  20. Holographic Optical Data Storage

    Science.gov (United States)

    Timucin, Dogan A.; Downie, John D.; Norvig, Peter (Technical Monitor)

    2000-01-01

    Although the basic idea may be traced back to the earlier X-ray diffraction studies of Sir W. L. Bragg, the holographic method as we know it was invented by D. Gabor in 1948 as a two-step lensless imaging technique to enhance the resolution of electron microscopy, for which he received the 1971 Nobel Prize in physics. The distinctive feature of holography is the recording of the object phase variations that carry the depth information, which is lost in conventional photography where only the intensity (= squared amplitude) distribution of an object is captured. Since all photosensitive media necessarily respond to the intensity incident upon them, an ingenious way had to be found to convert object phase into intensity variations, and Gabor achieved this by introducing a coherent reference wave along with the object wave during exposure. Gabor's in-line recording scheme, however, required the object in question to be largely transmissive, and could provide only marginal image quality due to unwanted terms simultaneously reconstructed along with the desired wavefront. Further handicapped by the lack of a strong coherent light source, optical holography thus seemed fated to remain just another scientific curiosity, until the field was revolutionized in the early 1960s by some major breakthroughs: the proposition and demonstration of the laser principle, the introduction of off-axis holography, and the invention of volume holography. Consequently, the remainder of that decade saw an exponential growth in research on theory, practice, and applications of holography. Today, holography not only boasts a wide variety of scientific and technical applications (e.g., holographic interferometry for strain, vibration, and flow analysis, microscopy and high-resolution imagery, imaging through distorting media, optical interconnects, holographic optical elements, optical neural networks, three-dimensional displays, data storage, etc.), but has become a prominent am advertising

  1. Holographic Phonons

    Science.gov (United States)

    Alberte, Lasma; Ammon, Martin; Jiménez-Alba, Amadeo; Baggioli, Matteo; Pujolàs, Oriol

    2018-04-01

    We present a class of holographic massive gravity models that realize a spontaneous breaking of translational symmetry—they exhibit transverse phonon modes whose speed relates to the elastic shear modulus according to elasticity theory. Massive gravity theories thus emerge as versatile and convenient theories to model generic types of translational symmetry breaking: explicit, spontaneous, and a mixture of both. The nature of the breaking is encoded in the radial dependence of the graviton mass. As an application of the model, we compute the temperature dependence of the shear modulus and find that it features a glasslike melting transition.

  2. Flowing holographic anyonic superfluid

    Science.gov (United States)

    Jokela, Niko; Lifschytz, Gilad; Lippert, Matthew

    2014-10-01

    We investigate the flow of a strongly coupled anyonic superfluid based on the holographic D3-D7' probe brane model. By analyzing the spectrum of fluctuations, we find the critical superfluid velocity, as a function of the temperature, at which the flow stops being dissipationless when flowing past a barrier. We find that at a larger velocity the flow becomes unstable even in the absence of a barrier.

  3. A robust holographic autofocusing criterion based on edge sparsity: comparison of Gini index and Tamura coefficient for holographic autofocusing based on the edge sparsity of the complex optical wavefront

    Science.gov (United States)

    Tamamitsu, Miu; Zhang, Yibo; Wang, Hongda; Wu, Yichen; Ozcan, Aydogan

    2018-02-01

    The Sparsity of the Gradient (SoG) is a robust autofocusing criterion for holography, where the gradient modulus of the complex refocused hologram is calculated, on which a sparsity metric is applied. Here, we compare two different choices of sparsity metrics used in SoG, specifically, the Gini index (GI) and the Tamura coefficient (TC), for holographic autofocusing on dense/connected or sparse samples. We provide a theoretical analysis predicting that for uniformly distributed image data, TC and GI exhibit similar behavior, while for naturally sparse images containing few high-valued signal entries and many low-valued noisy background pixels, TC is more sensitive to distribution changes in the signal and more resistive to background noise. These predictions are also confirmed by experimental results using SoG-based holographic autofocusing on dense and connected samples (such as stained breast tissue sections) as well as highly sparse samples (such as isolated Giardia lamblia cysts). Through these experiments, we found that ToG and GoG offer almost identical autofocusing performance on dense and connected samples, whereas for naturally sparse samples, GoG should be calculated on a relatively small region of interest (ROI) closely surrounding the object, while ToG offers more flexibility in choosing a larger ROI containing more background pixels.

  4. Holographic Aquaculture

    Science.gov (United States)

    Ian, Richard; King, Elisabeth

    1988-01-01

    Proposed is an exploratory study to verify the feasibility of an inexpensive micro-climate control system for both marine and freshwater pond and tank aquaculture, offering good control over water temperature, incident light flux, and bandwidth, combined with good energy efficiency. The proposed control system utilizes some familiar components of passive solar design, together with a new holographic glazing system which is currently being developed by, and proprietary to Advanced Environmental Research Group (AERG). The use of solar algae ponds and tanks to warm and purify water for fish and attached macroscopic marine algae culture is an ancient and effective technique, but limited seasonally and geographically by the availability of sunlight. Holographic Diffracting Structures (HDSs) can be made which passively track, accept and/or reject sunlight from a wide range of altitude and azimuth angles, and redirect and distribute light energy as desired (either directly or indirectly over water surface in an enclosed, insulated structure), effectively increasing insolation values by accepting sunlight which would not otherwise enter the structure.

  5. Microscopy

    Science.gov (United States)

    Patricia A. Moss; Les Groom

    2001-01-01

    Microscopy is the study and interpretation of images produced by a microscope. "Interpretation" is the keyword, because the microscope enables one to see structures that are too small or too close together to be resolved by the unaided eye. (The human eye cannot separate two points or lines that are closer together than 0.1 mm.) it is important to...

  6. Comparison of Gini index and Tamura coefficient for holographic autofocusing based on the edge sparsity of the complex optical wavefront

    KAUST Repository

    Tamamitsu, Miu

    2017-08-27

    The Sparsity of the Gradient (SoG) is a robust autofocusing criterion for holography, where the gradient modulus of the complex refocused hologram is calculated, on which a sparsity metric is applied. Here, we compare two different choices of sparsity metrics used in SoG, specifically, the Gini index (GI) and the Tamura coefficient (TC), for holographic autofocusing on dense/connected or sparse samples. We provide a theoretical analysis predicting that for uniformly distributed image data, TC and GI exhibit similar behavior, while for naturally sparse images containing few high-valued signal entries and many low-valued noisy background pixels, TC is more sensitive to distribution changes in the signal and more resistive to background noise. These predictions are also confirmed by experimental results using SoG-based holographic autofocusing on dense and connected samples (such as stained breast tissue sections) as well as highly sparse samples (such as isolated Giardia lamblia cysts). Through these experiments, we found that ToG and GoG offer almost identical autofocusing performance on dense and connected samples, whereas for naturally sparse samples, GoG should be calculated on a relatively small region of interest (ROI) closely surrounding the object, while ToG offers more flexibility in choosing a larger ROI containing more background pixels.

  7. The holographic optical micro-manipulation system based on counter-propagating beams

    Czech Academy of Sciences Publication Activity Database

    Čižmár, T.; Brzobohatý, Oto; Dholakia, K.; Zemánek, Pavel

    2011-01-01

    Roč. 8, č. 1 (2011), s. 50-56 ISSN 1612-2011 R&D Projects: GA ČR GA202/09/0348; GA MŠk(CZ) LC06007; GA MŠk OC08034; GA MŠk ED0017/01/01 Grant - others:EC(XE) COST MP0604 Institutional research plan: CEZ:AV0Z20650511 Keywords : holographic optical trapping * dual beam trap * spatial light modulator * optical rotator Subject RIV: BH - Optics, Masers, Lasers Impact factor: 9.970, year: 2011

  8. Speckle-based off-axis holographic detection for non-contact photoacoustic tomography

    Directory of Open Access Journals (Sweden)

    Buj C.

    2015-09-01

    Full Text Available A very fast innovative holographic off-axis non-contact detection method for Photoacoustic Tomography (PAT is introduced. It overcomes the main problems of most state-of-the-art photoacoustic imaging approaches that are long acquisition times and the requirement of acoustic contact. In order to increase the acquisition speed significantly, the surface displacements of the object, caused by the photoacoustic pressure waves, are measured interferometrically in two dimensions. Phase alterations in the observed speckle field are used to identify changes in the object’s topography. A sampling rate of up to 80 MHz is feasible, which reduces the occurrence of motion artefacts.

  9. Spectral and holographic characterization of new photochromic compounds based on substituted spiropyrans

    Science.gov (United States)

    Ciapurin, Igor V.; Robu, Stephan V.; Vlad, Lyudmila A.; Lessard, Roger A.; Tork, Amir; Lafond, Christophe; Bolte, Michel

    2001-06-01

    We report a new photochromic composite polymer consisting of poly-N-epoxypropylcarbazole (PEPC) polymeric matrix with a nitro-brome-substituted spiropyran (BNSP) photochromic dye. The PEPC + BNSP films can be considered as negative photochromic recording media. They are colored in the initial state and bleached upon irradiation within the visible spectra. When we placed the bleached samples to the darkness, they slowly revert to the colored form. This process has strong temperature dependence, so one can either 'freeze'' or accelerate changing of the current coloration state in the PEPC + BNSP. The experimental measurements are evaluated in conjunction with its potential applications for optical holographic recording in the visible spectral range. The real-time holographic recording procedure in PEPC + BNSP films was studied. The diffraction efficiency values reached the maximum of 23 percent at spatial frequency of 1600 line pairs per mm, during direct hologram recording with the 532 nm Coherent VERDI laser irradiation. Light exposures were ranged from 70 to 280 mJ/cm2. The investigated compounds have good perspectives for use in holography, two-photon optical data storage, electro-optics, and optical-limiting applications due to coupling of some unique properties such as high optical non-linearity, well charge transport, short response times, no-limiting resolution ability, etc.

  10. Design and optimization of cascaded DCG based holographic elements for spectrum-splitting PV systems

    Science.gov (United States)

    Wu, Yuechen; Chrysler, Benjamin; Pelaez, Silvana Ayala; Kostuk, Raymond K.

    2017-09-01

    In this work, the technique of designing and optimizing broadband volume transmission holograms using dichromate gelatin (DCG) is summarized for solar spectrum-splitting application. Spectrum splitting photovoltaic system uses a series of single bandgap PV cells that have different spectral conversion efficiency properties to more fully utilize the solar spectrum. In such a system, one or more high performance optical filters are usually required to split the solar spectrum and efficiently send them to the corresponding PV cells. An ideal spectral filter should have a rectangular shape with sharp transition wavelengths. DCG is a near ideal holographic material for solar applications as it can achieve high refractive index modulation, low absorption and scattering properties and long-term stability to solar exposure after sealing. In this research, a methodology of designing and modeling a transmission DCG hologram using coupled wave analysis for different PV bandgap combinations is described. To achieve a broad diffraction bandwidth and sharp cut-off wavelength, a cascaded structure of multiple thick holograms is described. A search algorithm is also developed to optimize both single and two-layer cascaded holographic spectrum splitters for the best bandgap combinations of two- and three-junction SSPV systems illuminated under the AM1.5 solar spectrum. The power conversion efficiencies of the optimized systems under the AM1.5 solar spectrum are then calculated using the detailed balance method, and shows an improvement compared with tandem structure.

  11. Laboratory-based recording of holographic fine structure in X-ray absorption anisotropy using polycapillary optics

    Energy Technology Data Exchange (ETDEWEB)

    Dabrowski, K.M. [Institute of Physics, Jagiellonian University, Reymonta 4, 30-059 Krakow (Poland); Korecki, P., E-mail: pawel.korecki@uj.edu.pl [Institute of Physics, Jagiellonian University, Reymonta 4, 30-059 Krakow (Poland)

    2012-08-15

    Highlights: Black-Right-Pointing-Pointer Holographic fine structures in X-ray absorption recorded using a tabletop setup. Black-Right-Pointing-Pointer Setup based on polycapillary collimating optics and an HOPG crystal. Black-Right-Pointing-Pointer Demonstration of element sensitivity by detection of X-ray fluorescence. Black-Right-Pointing-Pointer Potential of laboratory-based experiments for heavily doped crystals and thin films. - Abstract: A tabletop setup composed of a collimating polycapillary optics and a highly oriented pyrolytic graphite monochromator (HOPG) was characterized and used for recording two-dimensional maps of X-ray absorption anisotropy (XAA). XAA originates from interference of X-rays directly inside the sample. Depending on experimental conditions, fine structures in XAA can be interpreted in terms of X-ray holograms or X-ray standing waves and can be used for an element selective atomic-resolved structural analysis. The implementation of polycapillary optics resulted in a two-order of magnitude gain in the radiant intensity (photons/s/solid angle) as compared to a system without optics and enabled efficient recording of XAA with a resolution of 0.15 Degree-Sign for Mo K{alpha} radiation. Element sensitivity was demonstrated by acquisition of distinct XAA signals for Ga and As atoms in a GaAs (1 1 1) wafer by using X-ray fluorescence as a secondary signal. These results indicate the possibility of performing laboratory-based XAA experiments for heavily doped single crystals or thin films. So far, because of the weak holographic modulation of XAA, such experiments could be only performed using synchrotron radiation.

  12. Holographic characterization of colloidal particles in turbid media

    Science.gov (United States)

    Cheong, Fook Chiong; Kasimbeg, Priya; Ruffner, David B.; Hlaing, Ei Hnin; Blusewicz, Jaroslaw M.; Philips, Laura A.; Grier, David G.

    2017-10-01

    Holographic particle characterization uses in-line holographic microscopy and the Lorenz-Mie theory of light scattering to measure the diameter and the refractive index of individual colloidal particles in their native dispersions. This wealth of information has proved invaluable in fields as diverse as soft-matter physics, biopharmaceuticals, wastewater management, and food science but so far has been available only for dispersions in transparent media. Here, we demonstrate that holographic characterization can yield precise and accurate results even when the particles of interest are dispersed in turbid media. By elucidating how multiple light scattering contributes to image formation in holographic microscopy, we establish the range conditions under which holographic characterization can reliably probe turbid samples. We validate the technique with measurements on model colloidal spheres dispersed in commercial nanoparticle slurries.

  13. Laser adaptive holographic hydrophone

    Energy Technology Data Exchange (ETDEWEB)

    Romashko, R V; Kulchin, Yu N; Bezruk, M N; Ermolaev, S A [Institute of Automation and Control Processes, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok (Russian Federation)

    2016-03-31

    A new type of a laser hydrophone based on dynamic holograms, formed in a photorefractive crystal, is proposed and studied. It is shown that the use of dynamic holograms makes it unnecessary to use complex optical schemes and systems for electronic stabilisation of the interferometer operating point. This essentially simplifies the scheme of the laser hydrophone preserving its high sensitivity, which offers the possibility to use it under a strong variation of the environment parameters. The laser adaptive holographic hydrophone implemented at present possesses the sensitivity at a level of 3.3 mV Pa{sup -1} in the frequency range from 1 to 30 kHz. (laser hydrophones)

  14. Digital differential confocal microscopy based on spatial shift transformation.

    Science.gov (United States)

    Liu, J; Wang, Y; Liu, C; Wilson, T; Wang, H; Tan, J

    2014-11-01

    Differential confocal microscopy is a particularly powerful surface profilometry technique in industrial metrology due to its high axial sensitivity and insensitivity to noise. However, the practical implementation of the technique requires the accurate positioning of point detectors in three-dimensions. We describe a simple alternative based on spatial transformation of a through-focus series of images obtained from a homemade beam scanning confocal microscope. This digital differential confocal microscopy approach is described and compared with the traditional Differential confocal microscopy approach. The ease of use of the digital differential confocal microscopy system is illustrated by performing measurements on a 3D standard specimen. © 2014 The Authors Journal of Microscopy © 2014 Royal Microscopical Society.

  15. Review of quantitative phase-digital holographic microscopy: promising novel imaging technique to resolve neuronal network activity and identify cellular biomarkers of psychiatric disorders

    KAUST Repository

    Marquet, Pierre; Depeursinge, Christian; Magistretti, Pierre J.

    2014-01-01

    Quantitative phase microscopy (QPM) has recently emerged as a new powerful quantitative imaging technique well suited to noninvasively explore a transparent specimen with a nanometric axial sensitivity. In this review, we expose the recent

  16. Music holographic physiotherapy by laser

    Science.gov (United States)

    Liao, Changhuan

    1996-09-01

    Based on the relationship between music and nature, the paper compares laser and light with music sound on the principles of synergetics, describes music physically and objectively, and proposes a music holographic therapy by laser. Maybe it will have certain effects on mechanism study and clinical practice of the music therapy.

  17. Using digital holographic microscopy for simultaneous measurements of 3D near wall velocity and wall shear stress in a turbulent boundary layer

    Science.gov (United States)

    Sheng, J.; Malkiel, E.; Katz, J.

    2008-12-01

    A digital holographic microscope is used to simultaneously measure the instantaneous 3D flow structure in the inner part of a turbulent boundary layer over a smooth wall, and the spatial distribution of wall shear stresses. The measurements are performed in a fully developed turbulent channel flow within square duct, at a moderately high Reynolds number. The sample volume size is 90 × 145 × 90 wall units, and the spatial resolution of the measurements is 3 8 wall units in streamwise and spanwise directions and one wall unit in the wall-normal direction. The paper describes the data acquisition and analysis procedures, including the particle tracking method and associated method for matching of particle pairs. The uncertainty in velocity is estimated to be better than 1 mm/s, less than 0.05% of the free stream velocity, by comparing the statistics of the normalized velocity divergence to divergence obtained by randomly adding an error of 1 mm/s to the data. Spatial distributions of wall shear stresses are approximated with the least square fit of velocity measurements in the viscous sublayer. Mean flow profiles and statistics of velocity fluctuations agree very well with expectations. Joint probability density distributions of instantaneous spanwise and streamwise wall shear stresses demonstrate the significance of near-wall coherent structures. The near wall 3D flow structures are classified into three groups, the first containing a pair of counter-rotating, quasi streamwise vortices and high streak-like shear stresses; the second group is characterized by multiple streamwise vortices and little variations in wall stress; and the third group has no buffer layer structures.

  18. Fulgide-based WRE holographic materials: influence of the matrix on the fatigue process

    Science.gov (United States)

    Lessard, Roger A.; Lafond, Christophe; Darderian, Geraldine; Gardette, Jean-Luc; Rivaton, Agnes; Bolte, Michele

    2003-12-01

    The reversible reaction that takes place in the writable, readable, erasable (WRE) photosensitive materials involving fulgides (ABERCHROM 540 and 670) was investigated with a photochemical and holographic approach. It appeared that the fatigue of the photosensitive material was strongly dependent on the properties of the matrix used as a support. This was precisely established both by spectroscopic monitoring (λmax, absorbancemax, photo-stationary state) and by following the diffraction efficiency η values. There was a nice correlation between the evolution of the spectral features of C isomer and of the η values all along the WRE cycles. This combined approach was applied to four different supports: PVK, PMMA, PEPC/PS and epoxy RESIN. So for both fulgides: (1) PVK, frequently used in optics, appears as being the worst one. The starting fulgides were destroyed after only a few WRE cycles. This was assigned to the intrinsic photoaging of PVK whose absorption in UV domain is far from negligible and leads to the formation of radical species able to attack the fulgide. (2) Holograms recorded in PMMA and PEPC/PS present similar behavior with a loss of ~10% after 8 cycles. (3) Epoxy RESIN appears to be a very good candidate for these reversible systems; no fatigue was observed after 40 cycles. It has to be attributed to the matrix in which the detrimental rotation process, giving rise to the non photochromic Z isomer, is strongly inhibited.

  19. Holographic non-Gaussianity

    International Nuclear Information System (INIS)

    McFadden, Paul; Skenderis, Kostas

    2011-01-01

    We investigate the non-Gaussianity of primordial cosmological perturbations within our recently proposed holographic description of inflationary universes. We derive a holographic formula that determines the bispectrum of cosmological curvature perturbations in terms of correlation functions of a holographically dual three-dimensional non-gravitational quantum field theory (QFT). This allows us to compute the primordial bispectrum for a universe which started in a non-geometric holographic phase, using perturbative QFT calculations. Strikingly, for a class of models specified by a three-dimensional super-renormalisable QFT, the primordial bispectrum is of exactly the factorisable equilateral form with f NL equil. = 5/36, irrespective of the details of the dual QFT. A by-product of this investigation is a holographic formula for the three-point function of the trace of the stress-energy tensor along general holographic RG flows, which should have applications outside the remit of this work

  20. Using wire shaping techniques and holographic optics to optimize deposition characteristics in wire-based laser cladding.

    Science.gov (United States)

    Goffin, N J; Higginson, R L; Tyrer, J R

    2016-12-01

    In laser cladding, the potential benefits of wire feeding are considerable. Typical problems with the use of powder, such as gas entrapment, sub-100% material density and low deposition rate are all avoided with the use of wire. However, the use of a powder-based source material is the industry standard, with wire-based deposition generally regarded as an academic curiosity. This is because, although wire-based methods have been shown to be capable of superior quality results, the wire-based process is more difficult to control. In this work, the potential for wire shaping techniques, combined with existing holographic optical element knowledge, is investigated in order to further improve the processing characteristics. Experiments with pre-placed wire showed the ability of shaped wire to provide uniformity of wire melting compared with standard round wire, giving reduced power density requirements and superior control of clad track dilution. When feeding with flat wire, the resulting clad tracks showed a greater level of quality consistency and became less sensitive to alterations in processing conditions. In addition, a 22% increase in deposition rate was achieved. Stacking of multiple layers demonstrated the ability to create fully dense, three-dimensional structures, with directional metallurgical grain growth and uniform chemical structure.

  1. Origin of holographic dark energy models

    International Nuclear Information System (INIS)

    Myung, Yun Soo; Seo, Min-Gyun

    2009-01-01

    We investigate the origin of holographic dark energy models which were recently proposed to explain the dark energy-dominated universe. For this purpose, we introduce the spacetime foam uncertainty of δl≥l p α l α-1 . It was argued that the case of α=2/3 could describe the dark energy with infinite statistics, while the case of α=1/2 can describe the ordinary matter with Bose-Fermi statistics. However, two cases may lead to the holographic energy density if the latter recovers from the geometric mean of UV and IR scales. Hence the dark energy with infinite statistics based on the entropy bound is not an ingredient for deriving the holographic dark energy model. Furthermore, it is shown that the agegraphic dark energy models are the holographic dark energy model with different IR length scales

  2. Simple and robust image-based autofocusing for digital microscopy.

    Science.gov (United States)

    Yazdanfar, Siavash; Kenny, Kevin B; Tasimi, Krenar; Corwin, Alex D; Dixon, Elizabeth L; Filkins, Robert J

    2008-06-09

    A simple image-based autofocusing scheme for digital microscopy is demonstrated that uses as few as two intermediate images to bring the sample into focus. The algorithm is adapted to a commercial inverted microscope and used to automate brightfield and fluorescence imaging of histopathology tissue sections.

  3. Reducing microscopy-based malaria misdiagnosis in a low ...

    African Journals Online (AJOL)

    In this paper we report on the outcomes of a simple intervention that utilized a social entrepreneurship approach (SEA) to reduce misdiagnosis associated with hospital-based microscopy of malaria in a low-transmission area of rural Tanzania. A pre-post assessment was conducted on patients presenting to the hospital ...

  4. Boundary fitting based segmentation of fluorescence microscopy images

    Science.gov (United States)

    Lee, Soonam; Salama, Paul; Dunn, Kenneth W.; Delp, Edward J.

    2015-03-01

    Segmentation is a fundamental step in quantifying characteristics, such as volume, shape, and orientation of cells and/or tissue. However, quantification of these characteristics still poses a challenge due to the unique properties of microscopy volumes. This paper proposes a 2D segmentation method that utilizes a combination of adaptive and global thresholding, potentials, z direction refinement, branch pruning, end point matching, and boundary fitting methods to delineate tubular objects in microscopy volumes. Experimental results demonstrate that the proposed method achieves better performance than an active contours based scheme.

  5. Holographic Moire Contouring

    Science.gov (United States)

    Sciammarella, C. A.; Sainov, Ventseslav; Simova, Eli

    1990-04-01

    Theoretical analysis and experimental results on holographic moire contouring (HMC) of difussely reflecting objects are presented. The sensitivity and application constraints of the method are discussed. A high signal-to-noise ratio and contrast of the fringes is achieved through the use of high quality silver halide holographic plates HP-650. A good agreement between theoretical and experimental results is observed.

  6. Temporal overlap estimation based on interference spectrum in CARS microscopy

    Science.gov (United States)

    Zhang, Yongning; Jiang, Junfeng; Liu, Kun; Huang, Can; Wang, Shuang; Zhang, Xuezhi; Liu, Tiegen

    2018-01-01

    Coherent Anti-Stokes Raman Scattering (CARS) microscopy has attracted lots of attention because of the advantages, such as noninvasive, label-free, chemical specificity, intrinsic three-dimension spatial resolution and so on. However, the temporal overlap of pump and Stokes has not been solved owing to the ultrafast optical pulse used in CARS microscopy. We combine interference spectrum of residual pump in Stokes path and nonlinear Schrodinger equation (NLSE) to realize the temporal overlap of pump pulse and Stokes pulse. At first, based on the interference spectrum of pump pulse and residual pump in Stokes path, the optical delay is defined when optical path difference between pump path and Stokes path is zero. Then the relative optical delay between Stokes pulse and residual pump in PCF can be calculated by NLSE. According to the spectrum interference and NLSE, temporal overlap of pump pulse and Stokes pulse will be realized easily and the imaging speed will be improved in CARS microscopy.

  7. Polychromatic holographic plasma diagnostics

    International Nuclear Information System (INIS)

    Zhiglinskij, A.G.; Morozov, A.O.

    1992-01-01

    Review of holographic interferometry properties is performed and advantages of this method by plasma diagnostics are indicated. Main results obtained by the method of holographic interferometry in studies of various-type plasmas are considered. Special attention is paid to multiwave plasma diagnostics, the necessity of which is related as a rule to multicomponent composition of plasma. The eight laser and gas-discharge sources and holographic schemes, which make it possible to realize plasma polychromatic and holographic interferometry, are considered. The advantages of the method are demonstrated by examples of polychromatic holographic diagnostics of arc discharge and discharge in a hollow cathode. Review of theoretical works determining the applicability area of resonance polychromatic interferometry is carried out

  8. Automated seeding-based nuclei segmentation in nonlinear optical microscopy.

    Science.gov (United States)

    Medyukhina, Anna; Meyer, Tobias; Heuke, Sandro; Vogler, Nadine; Dietzek, Benjamin; Popp, Jürgen

    2013-10-01

    Nonlinear optical (NLO) microscopy based, e.g., on coherent anti-Stokes Raman scattering (CARS) or two-photon-excited fluorescence (TPEF) is a fast label-free imaging technique, with a great potential for biomedical applications. However, NLO microscopy as a diagnostic tool is still in its infancy; there is a lack of robust and durable nuclei segmentation methods capable of accurate image processing in cases of variable image contrast, nuclear density, and type of investigated tissue. Nonetheless, such algorithms specifically adapted to NLO microscopy present one prerequisite for the technology to be routinely used, e.g., in pathology or intraoperatively for surgical guidance. In this paper, we compare the applicability of different seeding and boundary detection methods to NLO microscopic images in order to develop an optimal seeding-based approach capable of accurate segmentation of both TPEF and CARS images. Among different methods, the Laplacian of Gaussian filter showed the best accuracy for the seeding of the image, while a modified seeded watershed segmentation was the most accurate in the task of boundary detection. The resulting combination of these methods followed by the verification of the detected nuclei performs high average sensitivity and specificity when applied to various types of NLO microscopy images.

  9. CCD-based thermoreflectance microscopy: principles and applications

    International Nuclear Information System (INIS)

    Farzaneh, M; Maize, K; Shakouri, A; Lueerssen, D; Summers, J A; Hudgings, Janice A; Mayer, P M; Ram, R J; Raad, P E; Pipe, K P

    2009-01-01

    CCD-based thermoreflectance microscopy has emerged as a high resolution, non-contact imaging technique for thermal profiling and performance and reliability analysis of numerous electronic and optoelectronic devices at the micro-scale. This thermography technique, which is based on measuring the relative change in reflectivity of the device surface as a function of change in temperature, provides high-resolution thermal images that are useful for hot spot detection and failure analysis, mapping of temperature distribution, measurement of thermal transient, optical characterization of photonic devices and measurement of thermal conductivity in thin films. In this paper we review the basic physical principle behind thermoreflectance as a thermography tool, discuss the experimental setup, resolutions achieved, signal processing procedures and calibration techniques, and review the current applications of CCD-based thermoreflectance microscopy in various devices. (topical review)

  10. Comparison of Gini index and Tamura coefficient for holographic autofocusing based on the edge sparsity of the complex optical wavefront

    KAUST Repository

    Tamamitsu, Miu; Zhang, Yibo; Wang, Hongda; Wu, Yichen; Ozcan, Aydogan

    2017-01-01

    of sparsity metrics used in SoG, specifically, the Gini index (GI) and the Tamura coefficient (TC), for holographic autofocusing on dense/connected or sparse samples. We provide a theoretical analysis predicting that for uniformly distributed image data, TC

  11. Weak-interacting holographic QCD

    International Nuclear Information System (INIS)

    Gazit, D.; Yee, H.-U.

    2008-06-01

    We propose a simple prescription for including low-energy weak-interactions into the frame- work of holographic QCD, based on the standard AdS/CFT dictionary of double-trace deformations. As our proposal enables us to calculate various electro-weak observables involving strongly coupled QCD, it opens a new perspective on phenomenological applications of holographic QCD. We illustrate efficiency and usefulness of our method by performing a few exemplar calculations; neutron beta decay, charged pion weak decay, and meson-nucleon parity non-conserving (PNC) couplings. The idea is general enough to be implemented in both Sakai-Sugimoto as well as Hard/Soft Wall models. (author)

  12. Solid-State Quantum Computer Based on Scanning Tunneling Microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Berman, G. P.; Brown, G. W.; Hawley, M. E.; Tsifrinovich, V. I.

    2001-08-27

    We propose a solid-state nuclear-spin quantum computer based on application of scanning tunneling microscopy (STM) and well-developed silicon technology. It requires the measurement of tunneling-current modulation caused by the Larmor precession of a single electron spin. Our envisioned STM quantum computer would operate at the high magnetic field ({approx}10 T) and at low temperature {approx}1 K .

  13. Solid-State Quantum Computer Based on Scanning Tunneling Microscopy

    International Nuclear Information System (INIS)

    Berman, G. P.; Brown, G. W.; Hawley, M. E.; Tsifrinovich, V. I.

    2001-01-01

    We propose a solid-state nuclear-spin quantum computer based on application of scanning tunneling microscopy (STM) and well-developed silicon technology. It requires the measurement of tunneling-current modulation caused by the Larmor precession of a single electron spin. Our envisioned STM quantum computer would operate at the high magnetic field (∼10 T) and at low temperature ∼1 K

  14. Holographic interferometry in construction analysis

    Energy Technology Data Exchange (ETDEWEB)

    Hartikainen, T.

    1995-12-31

    In this work techniques for visualizing phase and opaque objects by ruby laser interferometry are introduced. A leakage flow as a phase object is studied by holographic interferometry and the intensity distribution of the interferograms presenting the leakage flow are computer-simulated. A qualitative and quantitative analysis of the leakage flow is made. The analysis is based on the experimental and theoretical results presented in this work. The holographic setup and the double pass method for visualizing leakage flow are explained. A vibrating iron plate is the opaque object. Transient impact waves are generated by a pistol bullet on the iron plate and visualized by holographic interferometry. An apparatus with the capability of detecting and calculating the delays necessary for laser triggering is introduced. A time series of interferograms presenting elastic wave formation in an iron plate is shown. A computer-simulation of the intensity distributions of these interferograms is made. An analysis based on the computer-simulation and the experimental data of the transient elastic wave is carried out and the results are presented. (author)

  15. Pulse holographic measurement techniques

    International Nuclear Information System (INIS)

    Kim, Cheol Jung; Baik, Seong Hoon; Hong, Seok Kyung; Kim, Jeong Moog; Kim, Duk Hyun

    1992-01-01

    With the development of laser, remote inspection techniques using laser have been growing on. The inspection and measurement techniques by pulse holography are well-established technique for precise measurement, and widely used in various fields of industry now. In nuclear industry, this technology is practically used because holographic inspection is remote, noncontact, and precise measurement technique. In relation to remote inspection technology in nuclear industry, state-of-the art of pulse HNDT (Holographic non-destructive testing) and holographic measurement techniques are examined. First of all, the fundamental principles as well as practical problems for applications are briefly described. The fields of pulse holography have been divided into the HNDT, flow visualization and distribution study, and other application techniques. Additionally holographic particle study, bubble chamber holography, and applications to other visualization techniques are described. Lastly, the current status for the researches and applications of pulse holography to nuclear industry which are carried out actively in Europe and USA, is described. (Author)

  16. Use of holographic environment in business and educational application

    International Nuclear Information System (INIS)

    Rajput, A.Q.K.; Shaikh, M.Z.; Khanzada, T.J.S.

    2003-01-01

    Holographic environment is based on high-equipped Multimedia information systems. These are based on the evolving powers of computers to handle huge volume of information. Holographic environment is a simulated environment that allows the user to touch and interact with projections, which are derived from the distant real environment. A new communications technology is being developed that will facilitate to interact inside a simulated environment, even if you are thousands of miles apart. This is done with enhancing the electro-holography, which is the computer based generation of diffraction fringes from 3D input data and the display of the reconstructed object in real-time. This research paper presents the design and development of holographic environment for reduction of distances in business and educational applications. The Holographic Environment development with the use of multimedia information systems is discussed. In Particular the characteristics of holographic data and the current research results in the area of real time holographic display systems are spanned. The Technical components of holographic system are also encountered. Finally, issues of improvement in efficiency of Holographic Environments by compression of data are presented along with its utilization for educational and business applications. (author)

  17. Multidirectional Image Sensing for Microscopy Based on a Rotatable Robot

    Directory of Open Access Journals (Sweden)

    Yajing Shen

    2015-12-01

    Full Text Available Image sensing at a small scale is essentially important in many fields, including microsample observation, defect inspection, material characterization and so on. However, nowadays, multi-directional micro object imaging is still very challenging due to the limited field of view (FOV of microscopes. This paper reports a novel approach for multi-directional image sensing in microscopes by developing a rotatable robot. First, a robot with endless rotation ability is designed and integrated with the microscope. Then, the micro object is aligned to the rotation axis of the robot automatically based on the proposed forward-backward alignment strategy. After that, multi-directional images of the sample can be obtained by rotating the robot within one revolution under the microscope. To demonstrate the versatility of this approach, we view various types of micro samples from multiple directions in both optical microscopy and scanning electron microscopy, and panoramic images of the samples are processed as well. The proposed method paves a new way for the microscopy image sensing, and we believe it could have significant impact in many fields, especially for sample detection, manipulation and characterization at a small scale.

  18. Optimal model-based sensorless adaptive optics for epifluorescence microscopy.

    Science.gov (United States)

    Pozzi, Paolo; Soloviev, Oleg; Wilding, Dean; Vdovin, Gleb; Verhaegen, Michel

    2018-01-01

    We report on a universal sample-independent sensorless adaptive optics method, based on modal optimization of the second moment of the fluorescence emission from a point-like excitation. Our method employs a sample-independent precalibration, performed only once for the particular system, to establish the direct relation between the image quality and the aberration. The method is potentially applicable to any form of microscopy with epifluorescence detection, including the practically important case of incoherent fluorescence emission from a three dimensional object, through minor hardware modifications. We have applied the technique successfully to a widefield epifluorescence microscope and to a multiaperture confocal microscope.

  19. 3D holographic polymer photonic crystal for superprism application

    Science.gov (United States)

    Chen, Jiaqi; Jiang, Wei; Chen, Xiaonan; Wang, Li; Zhang, Sasa; Chen, Ray T.

    2007-02-01

    Photonic crystal based superprism offers a new way to design new optical components for beam steering and DWDM application. 3D photonic crystals are especially attractive as they could offer more control of the light beam based on the needs. A polygonal prism based holographic fabrication method has been demonstrated for a three-dimensional face-centered-cubic (FCC)-type submicron polymer photonic crystal using SU8 as the photo-sensitive material. Therefore antivibration equipment and complicated optical alignment system are not needed and the requirement for the coherence of the laser source is relaxed compared with the traditional holographic setup. By changing the top-cut prism structure, the polarization of the laser beam, the exposure and development conditions we can achieve different kinds of triclinic or orthorhombic photonic crystals on demand. Special fabrication treatments have been introduced to ensure the survivability of the fabricated large area (cm2) nano-structures. Scanning electron microscopy and diffraction results proved the good uniformity of the fabricated structures. With the proper design of the refraction prism we have achieved a partial bandgap for S+C band (1460-1565nm) in the [111] direction. The transmission and reflection spectra obtained by Fourier transform infrared spectroscopy (FTIR) are in good agreement with simulated band structure. The superprism effects around 1550nm wavelength for the fabricated 3D polymer photonic crystal have been theoretically calculated and such effects can be used for beam steering purpose.

  20. Research on copying system of dynamic multiplex holographic stereograms

    Science.gov (United States)

    Fu, Huaiping; Yang, Hong; Zheng, Tong

    2003-05-01

    The most important advantage of holographic stereograms over conventional hologram is that they can produce 3D images at any desired scale with movement, holographers in many countries involved in the studies towards it. We began our works in the early 80's and accomplished two research projects automatic system for making synthetic holograms and multiplex synthetic rainbow holograms, Based on these works, a large scale holographic stereogram of an animated goldfish was made by us for practical advertisement. In order to meet the needs of the market, a copying system for making multiplex holographic stereograms, and a special kind of silver halide holographic film developed by us recently. The characteristic of the copying system and the property of the special silver-halide emulsion are introduced in this paper.

  1. Example-Based Super-Resolution Fluorescence Microscopy.

    Science.gov (United States)

    Jia, Shu; Han, Boran; Kutz, J Nathan

    2018-04-23

    Capturing biological dynamics with high spatiotemporal resolution demands the advancement in imaging technologies. Super-resolution fluorescence microscopy offers spatial resolution surpassing the diffraction limit to resolve near-molecular-level details. While various strategies have been reported to improve the temporal resolution of super-resolution imaging, all super-resolution techniques are still fundamentally limited by the trade-off associated with the longer image acquisition time that is needed to achieve higher spatial information. Here, we demonstrated an example-based, computational method that aims to obtain super-resolution images using conventional imaging without increasing the imaging time. With a low-resolution image input, the method provides an estimate of its super-resolution image based on an example database that contains super- and low-resolution image pairs of biological structures of interest. The computational imaging of cellular microtubules agrees approximately with the experimental super-resolution STORM results. This new approach may offer potential improvements in temporal resolution for experimental super-resolution fluorescence microscopy and provide a new path for large-data aided biomedical imaging.

  2. Object tracking mask-based NLUT on GPUs for real-time generation of holographic videos of three-dimensional scenes.

    Science.gov (United States)

    Kwon, M-W; Kim, S-C; Yoon, S-E; Ho, Y-S; Kim, E-S

    2015-02-09

    A new object tracking mask-based novel-look-up-table (OTM-NLUT) method is proposed and implemented on graphics-processing-units (GPUs) for real-time generation of holographic videos of three-dimensional (3-D) scenes. Since the proposed method is designed to be matched with software and memory structures of the GPU, the number of compute-unified-device-architecture (CUDA) kernel function calls and the computer-generated hologram (CGH) buffer size of the proposed method have been significantly reduced. It therefore results in a great increase of the computational speed of the proposed method and enables real-time generation of CGH patterns of 3-D scenes. Experimental results show that the proposed method can generate 31.1 frames of Fresnel CGH patterns with 1,920 × 1,080 pixels per second, on average, for three test 3-D video scenarios with 12,666 object points on three GPU boards of NVIDIA GTX TITAN, and confirm the feasibility of the proposed method in the practical application of electro-holographic 3-D displays.

  3. Holographic Spherically Symmetric Metrics

    Science.gov (United States)

    Petri, Michael

    The holographic principle (HP) conjectures, that the maximum number of degrees of freedom of any realistic physical system is proportional to the system's boundary area. The HP has its roots in the study of black holes. It has recently been applied to cosmological solutions. In this article we apply the HP to spherically symmetric static space-times. We find that any regular spherically symmetric object saturating the HP is subject to tight constraints on the (interior) metric, energy-density, temperature and entropy-density. Whenever gravity can be described by a metric theory, gravity is macroscopically scale invariant and the laws of thermodynamics hold locally and globally, the (interior) metric of a regular holographic object is uniquely determined up to a constant factor and the interior matter-state must follow well defined scaling relations. When the metric theory of gravity is general relativity, the interior matter has an overall string equation of state (EOS) and a unique total energy-density. Thus the holographic metric derived in this article can serve as simple interior 4D realization of Mathur's string fuzzball proposal. Some properties of the holographic metric and its possible experimental verification are discussed. The geodesics of the holographic metric describe an isotropically expanding (or contracting) universe with a nearly homogeneous matter-distribution within the local Hubble volume. Due to the overall string EOS the active gravitational mass-density is zero, resulting in a coasting expansion with Ht = 1, which is compatible with the recent GRB-data.

  4. Phase holographic material with diffusion enhancement based on 1,2-naphthoquinone

    International Nuclear Information System (INIS)

    Gritsai, Y; Goldenberg, L M; Stumpe, J

    2010-01-01

    A new material based on 1,2-naphthoquinone in polymethylmethacrylate is proposed for phase holograms showing diffusion enhancement. The spectral sensitivity of this material allows forming holograms using 488–530 nm wavelengths, while the hologram reconstruction can be implemented at 633 nm. The amplitude of the refractive index modulation achieved makes it possible to reach diffraction efficiencies close to 100% at a thickness of several hundreds of µm. It was found by means of UV–visible and NMR spectroscopy that the diffusion of 1,2-naphthoquinone caused by photoproduct attachment to macromolecules of a polymer matrix is the main reason for the hologram enhancement. A relatively high diffusion coefficient (4.8 × 10 −17 m 2 s −1 at 50 °C) was measured, leading to fast enhancement at moderate temperature

  5. Ultramicrosensors based on transition metal hexacyanoferrates for scanning electrochemical microscopy

    Directory of Open Access Journals (Sweden)

    Maria A. Komkova

    2013-10-01

    Full Text Available We report here a way for improving the stability of ultramicroelectrodes (UME based on hexacyanoferrate-modified metals for the detection of hydrogen peroxide. The most stable sensors were obtained by electrochemical deposition of six layers of hexacyanoferrates (HCF, more specifically, an alternating pattern of three layers of Prussian Blue and three layers of Ni–HCF. The microelectrodes modified with mixed layers were continuously monitored in 1 mM hydrogen peroxide and proved to be stable for more than 5 h under these conditions. The mixed layer microelectrodes exhibited a stability which is five times as high as the stability of conventional Prussian Blue-modified UMEs. The sensitivity of the mixed layer sensor was 0.32 A·M−1·cm−2, and the detection limit was 10 µM. The mixed layer-based UMEs were used as sensors in scanning electrochemical microscopy (SECM experiments for imaging of hydrogen peroxide evolution.

  6. Magnetoelectric force microscopy based on magnetic force microscopy with modulated electric field.

    Science.gov (United States)

    Geng, Yanan; Wu, Weida

    2014-05-01

    We present the realization of a mesoscopic imaging technique, namely, the Magnetoelectric Force Microscopy (MeFM), for visualization of local magnetoelectric effect. The basic principle of MeFM is the lock-in detection of local magnetoelectric response, i.e., the electric field-induced magnetization, using magnetic force microscopy. We demonstrate MeFM capability by visualizing magnetoelectric domains on single crystals of multiferroic hexagonal manganites. Results of several control experiments exclude artifacts or extrinsic origins of the MeFM signal. The parameters are tuned to optimize the signal to noise ratio.

  7. Anomalous transport and holographic momentum relaxation

    Science.gov (United States)

    Copetti, Christian; Fernández-Pendás, Jorge; Landsteiner, Karl; Megías, Eugenio

    2017-09-01

    The chiral magnetic and vortical effects denote the generation of dissipationless currents due to magnetic fields or rotation. They can be studied in holographic models with Chern-Simons couplings dual to anomalies in field theory. We study a holographic model with translation symmetry breaking based on linear massless scalar field backgrounds. We compute the electric DC conductivity and find that it can vanish for certain values of the translation symmetry breaking couplings. Then we compute the chiral magnetic and chiral vortical conductivities. They are completely independent of the holographic disorder couplings and take the usual values in terms of chemical potential and temperature. To arrive at this result we suggest a new definition of energy-momentum tensor in presence of the gravitational Chern-Simons coupling.

  8. ASIC chipset design to generate block-based complex holographic video.

    Science.gov (United States)

    Seo, Young-Ho; Lee, Yoon-Hyuk; Kim, Dong-Wook

    2017-03-20

    In this paper, we propose a new hardware architecture implemented as a very large scaled integrated circuit by using an application-specific integrated circuit technology, where block-based calculations are used to generate holograms. The proposed hardware is structured to produce a part of a hologram in the block units in parallel. A block of a hologram is calculated using an object point, and then the calculation is repeated for all object points to obtain intermediate results that are accumulated to produce the final block of a hologram. This structure can be used to produce holograms of various sizes in real time with optimized memory access. The proposed hardware was implemented using the Hynix 0.18 μm CMOS technology of Magna Chip, Inc., and it has about 448 K gate counts and a silicon size of 3.592  mm×3.592  mm. It can generate complex holograms and operate in a stable manner at a clock frequency of 200 MHz.

  9. Holographic Entanglement Entropy

    CERN Document Server

    Rangamani, Mukund

    2016-01-01

    We review the developments in the past decade on holographic entanglement entropy, a subject that has garnered much attention owing to its potential to teach us about the emergence of spacetime in holography. We provide an introduction to the concept of entanglement entropy in quantum field theories, review the holographic proposals for computing the same, providing some justification for where these proposals arise from in the first two parts. The final part addresses recent developments linking entanglement and geometry. We provide an overview of the various arguments and technical developments that teach us how to use field theory entanglement to detect geometry. Our discussion is by design eclectic; we have chosen to focus on developments that appear to us most promising for further insights into the holographic map. This is a preliminary draft of a few chapters of a book which will appear sometime in the near future, to be published by Springer. The book in addition contains a discussion of application o...

  10. The holographic universe

    CERN Document Server

    Talbot, Michael

    1991-01-01

    'There is evidence to suggest that our world and everything in it - from snowflakes to maple trees to falling stars and spinning electrons - are only ghostly images, projections from a level of reality literally beyond both space and time.' This is the astonishing idea behind the holographic theory of the universe, pioneered by two eminent thinkers: physicist David Bohm, a former protege of Albert Einstein, and quantum physicist Karl Pribram. The holographic theory of the universe encompasses consciousness and reality as we know them, but can also explain such hitherto unexplained phenomena as telepathy, out-of-body experiences and even miraculous healing. In this remarkable book, Michael Talbot reveals the extraordinary depth and power of the holographic theory of the universe, illustrating how it makes sense of the entire range of experiences within our universe - and in other universes beyond our own.

  11. Computer generated holographic microtags

    International Nuclear Information System (INIS)

    Sweatt, W.C.

    1998-01-01

    A microlithographic tag comprising an array of individual computer generated holographic patches having feature sizes between 250 and 75 nanometers is disclosed. The tag is a composite hologram made up of the individual holographic patches and contains identifying information when read out with a laser of the proper wavelength and at the proper angles of probing and reading. The patches are fabricated in a steep angle Littrow readout geometry to maximize returns in the -1 diffracted order. The tags are useful as anti-counterfeiting markers because of the extreme difficulty in reproducing them. 5 figs

  12. Development of Tuning Fork Based Probes for Atomic Force Microscopy

    Science.gov (United States)

    Jalilian, Romaneh; Yazdanpanah, Mehdi M.; Torrez, Neil; Alizadeh, Amirali; Askari, Davood

    2014-03-01

    This article reports on the development of tuning fork-based AFM/STM probes in NaugaNeedles LLC for use in atomic force microscopy. These probes can be mounted on different carriers per customers' request. (e.g., RHK carrier, Omicron carrier, and tuning fork on a Sapphire disk). We are able to design and engineer tuning forks on any type of carrier used in the market. We can attach three types of tips on the edge of a tuning fork prong (i.e., growing Ag2Ga nanoneedles at any arbitrary angle, cantilever of AFM tip, and tungsten wire) with lengths from 100-500 μm. The nanoneedle is located vertical to the fork. Using a suitable insulation and metallic coating, we can make QPlus sensors that can detect tunneling current during the AFM scan. To make Qplus sensors, the entire quartz fork will be coated with an insulating material, before attaching the nanoneedle. Then, the top edge of one prong is coated with a thin layer of conductive metal and the nanoneedle is attached to the fork end of the metal coated prong. The metal coating provides electrical connection to the tip for tunneling current readout and to the electrodes and used to read the QPlus current. Since the amount of mass added to the fork is minimal, the resonance frequency spectrum does not change and still remains around 32.6 KHz and the Q factor is around 1,200 in ambient condition. These probes can enhance the performance of tuning fork based atomic microscopy.

  13. Spectroscopic scanning tunneling microscopy insights into Fe-based superconductors

    International Nuclear Information System (INIS)

    Hoffman, Jennifer E

    2011-01-01

    In the first three years since the discovery of Fe-based high T c superconductors, scanning tunneling microscopy (STM) and spectroscopy have shed light on three important questions. First, STM has demonstrated the complexity of the pairing symmetry in Fe-based materials. Phase-sensitive quasiparticle interference (QPI) imaging and low temperature spectroscopy have shown that the pairing order parameter varies from nodal to nodeless s± within a single family, FeTe 1-x Se x . Second, STM has imaged C4 → C2 symmetry breaking in the electronic states of both parent and superconducting materials. As a local probe, STM is in a strong position to understand the interactions between these broken symmetry states and superconductivity. Finally, STM has been used to image the vortex state, giving insights into the technical problem of vortex pinning, and the fundamental problem of the competing states introduced when superconductivity is locally quenched by a magnetic field. Here we give a pedagogical introduction to STM and QPI imaging, discuss the specific challenges associated with extracting bulk properties from the study of surfaces, and report on progress made in understanding Fe-based superconductors using STM techniques.

  14. Holographic optical security systems

    Science.gov (United States)

    Fagan, William F.

    1990-06-01

    One of the most successful applications of Holography,in recent years,has been its use as an optical security technique.Indeed the general public's awareness of holograms has been greatly enhanced by the incorporation of holographic elements into the VISA and MASTERCHARGE credit cards.Optical techniques related to Holography,are also being used to protect the currencies of several countries against the counterfeiter. The mass production of high quality holographic images is by no means a trivial task as a considerable degree of expertise is required together with an optical laboratory and embossing machinery.This paper will present an overview of the principal holographic and related optical techniques used for security purposes.Worldwide, over thirty companies are involved in the production of security elements utilising holographic and related optical technologies.Counterfeiting of many products is a major criminal activity with severe consequences not only for the manufacturer but for the public in general as defective automobile parts,aircraft components,and pharmaceutical products, to cite only a few of the more prominent examples,have at one time or another been illegally copied.

  15. Holographic renormalization and supersymmetry

    Energy Technology Data Exchange (ETDEWEB)

    Genolini, Pietro Benetti [Mathematical Institute, University of Oxford,Woodstock Road, Oxford OX2 6GG (United Kingdom); Cassani, Davide [LPTHE, Sorbonne Universités UPMC Paris 6 and CNRS, UMR 7589,F-75005, Paris (France); Martelli, Dario [Department of Mathematics, King’s College London,The Strand, London, WC2R 2LS (United Kingdom); Sparks, James [Mathematical Institute, University of Oxford,Woodstock Road, Oxford OX2 6GG (United Kingdom)

    2017-02-27

    Holographic renormalization is a systematic procedure for regulating divergences in observables in asymptotically locally AdS spacetimes. For dual boundary field theories which are supersymmetric it is natural to ask whether this defines a supersymmetric renormalization scheme. Recent results in localization have brought this question into sharp focus: rigid supersymmetry on a curved boundary requires specific geometric structures, and general arguments imply that BPS observables, such as the partition function, are invariant under certain deformations of these structures. One can then ask if the dual holographic observables are similarly invariant. We study this question in minimal N=2 gauged supergravity in four and five dimensions. In four dimensions we show that holographic renormalization precisely reproduces the expected field theory results. In five dimensions we find that no choice of standard holographic counterterms is compatible with supersymmetry, which leads us to introduce novel finite boundary terms. For a class of solutions satisfying certain topological assumptions we provide some independent tests of these new boundary terms, in particular showing that they reproduce the expected VEVs of conserved charges.

  16. The holographic entropy cone

    Energy Technology Data Exchange (ETDEWEB)

    Bao, Ning [Institute for Quantum Information and Matter, California Institute of Technology,Pasadena, CA 91125 (United States); Walter Burke Institute for Theoretical Physics, California Institute of Technology,452-48, Pasadena, CA 91125 (United States); Nezami, Sepehr [Stanford Institute for Theoretical Physics, Stanford University,Stanford, CA 94305 (United States); Ooguri, Hirosi [Walter Burke Institute for Theoretical Physics, California Institute of Technology,452-48, Pasadena, CA 91125 (United States); Kavli Institute for the Physics and Mathematics of the Universe, University of Tokyo,Kashiwa 277-8583 (Japan); Stoica, Bogdan [Walter Burke Institute for Theoretical Physics, California Institute of Technology,452-48, Pasadena, CA 91125 (United States); Sully, James [Theory Group, SLAC National Accelerator Laboratory, Stanford University,Menlo Park, CA 94025 (United States); Walter, Michael [Stanford Institute for Theoretical Physics, Stanford University,Stanford, CA 94305 (United States)

    2015-09-21

    We initiate a systematic enumeration and classification of entropy inequalities satisfied by the Ryu-Takayanagi formula for conformal field theory states with smooth holographic dual geometries. For 2, 3, and 4 regions, we prove that the strong subadditivity and the monogamy of mutual information give the complete set of inequalities. This is in contrast to the situation for generic quantum systems, where a complete set of entropy inequalities is not known for 4 or more regions. We also find an infinite new family of inequalities applicable to 5 or more regions. The set of all holographic entropy inequalities bounds the phase space of Ryu-Takayanagi entropies, defining the holographic entropy cone. We characterize this entropy cone by reducing geometries to minimal graph models that encode the possible cutting and gluing relations of minimal surfaces. We find that, for a fixed number of regions, there are only finitely many independent entropy inequalities. To establish new holographic entropy inequalities, we introduce a combinatorial proof technique that may also be of independent interest in Riemannian geometry and graph theory.

  17. The holographic entropy cone

    International Nuclear Information System (INIS)

    Bao, Ning; Nezami, Sepehr; Ooguri, Hirosi; Stoica, Bogdan; Sully, James; Walter, Michael

    2015-01-01

    We initiate a systematic enumeration and classification of entropy inequalities satisfied by the Ryu-Takayanagi formula for conformal field theory states with smooth holographic dual geometries. For 2, 3, and 4 regions, we prove that the strong subadditivity and the monogamy of mutual information give the complete set of inequalities. This is in contrast to the situation for generic quantum systems, where a complete set of entropy inequalities is not known for 4 or more regions. We also find an infinite new family of inequalities applicable to 5 or more regions. The set of all holographic entropy inequalities bounds the phase space of Ryu-Takayanagi entropies, defining the holographic entropy cone. We characterize this entropy cone by reducing geometries to minimal graph models that encode the possible cutting and gluing relations of minimal surfaces. We find that, for a fixed number of regions, there are only finitely many independent entropy inequalities. To establish new holographic entropy inequalities, we introduce a combinatorial proof technique that may also be of independent interest in Riemannian geometry and graph theory.

  18. Projection multiplex recording of computer-synthesised one-dimensional Fourier holograms for holographic memory systems: mathematical and experimental modelling

    Energy Technology Data Exchange (ETDEWEB)

    Betin, A Yu; Bobrinev, V I; Verenikina, N M; Donchenko, S S; Odinokov, S B [Research Institute ' Radiotronics and Laser Engineering' , Bauman Moscow State Technical University, Moscow (Russian Federation); Evtikhiev, N N; Zlokazov, E Yu; Starikov, S N; Starikov, R S [National Reseach Nuclear University MEPhI (Moscow Engineering Physics Institute), Moscow (Russian Federation)

    2015-08-31

    A multiplex method of recording computer-synthesised one-dimensional Fourier holograms intended for holographic memory devices is proposed. The method potentially allows increasing the recording density in the previously proposed holographic memory system based on the computer synthesis and projection recording of data page holograms. (holographic memory)

  19. Optimisation-based wavefront sensorless adaptive optics for microscopy

    NARCIS (Netherlands)

    Antonello, J.

    2014-01-01

    Microscopy is an essential tool for life sciences. Thanks to the development of confocal and multiphoton microscopy, scientists are able to obtain high-resolution 3D views of biological specimens. Nevertheless, spatial variations in the index of refraction within specimens cause aberrations that

  20. Saturated virtual fluorescence emission difference microscopy based on detector array

    Science.gov (United States)

    Liu, Shaocong; Sun, Shiyi; Kuang, Cuifang; Ge, Baoliang; Wang, Wensheng; Liu, Xu

    2017-07-01

    Virtual fluorescence emission difference microscopy (vFED) has been proposed recently to enhance the lateral resolution of confocal microscopy with a detector array, implemented by scanning a doughnut-shaped pattern. Theoretically, the resolution can be enhanced by around 1.3-fold compared with that in confocal microscopy. For further improvement of the resolving ability of vFED, a novel method is presented utilizing fluorescence saturation for super-resolution imaging, which we called saturated virtual fluorescence emission difference microscopy (svFED). With a point detector array, matched solid and hollow point spread functions (PSF) can be obtained by photon reassignment, and the difference results between them can be used to boost the transverse resolution. Results show that the diffraction barrier can be surpassed by at least 34% compared with that in vFED and the resolution is around 2-fold higher than that in confocal microscopy.

  1. Optimal micro-mirror tilt angle and sync mark design for digital micro-mirror device based collinear holographic data storage system.

    Science.gov (United States)

    Liu, Jinpeng; Horimai, Hideyoshi; Lin, Xiao; Liu, Jinyan; Huang, Yong; Tan, Xiaodi

    2017-06-01

    The collinear holographic data storage system (CHDSS) is a very promising storage system due to its large storage capacities and high transfer rates in the era of big data. The digital micro-mirror device (DMD) as a spatial light modulator is the key device of the CHDSS due to its high speed, high precision, and broadband working range. To improve the system stability and performance, an optimal micro-mirror tilt angle was theoretically calculated and experimentally confirmed by analyzing the relationship between the tilt angle of the micro-mirror on the DMD and the power profiles of diffraction patterns of the DMD at the Fourier plane. In addition, we proposed a novel chess board sync mark design in the data page to reduce the system bit error rate in circumstances of reduced aperture required to decrease noise and median exposure amount. It will provide practical guidance for future DMD based CHDSS development.

  2. Holographic interferometry using a digital photo-camera

    International Nuclear Information System (INIS)

    Sekanina, H.; Hledik, S.

    2001-01-01

    The possibilities of running digital holographic interferometry using commonly available compact digital zoom photo-cameras are studied. The recently developed holographic setup, suitable especially for digital photo-cameras equipped with an un detachable object lens, is used. The method described enables a simple and straightforward way of both recording and reconstructing of a digital holographic interferograms. The feasibility of the new method is verified by digital reconstruction of the interferograms acquired, using a numerical code based on the fast Fourier transform. Experimental results obtained are presented and discussed. (authors)

  3. Holographic conductivity of holographic superconductors with higher-order corrections

    Energy Technology Data Exchange (ETDEWEB)

    Sheykhi, Ahmad [Shiraz University, Physics Department and Biruni Observatory, College of Sciences, Shiraz (Iran, Islamic Republic of); Research Institute for Astronomy and Astrophysics of Maragha (RIAAM), Maragha (Iran, Islamic Republic of); Ghazanfari, Afsoon; Dehyadegari, Amin [Shiraz University, Physics Department and Biruni Observatory, College of Sciences, Shiraz (Iran, Islamic Republic of)

    2018-02-15

    We analytically and numerically disclose the effects of the higher-order correction terms in the gravity and in the gauge field on the properties of s-wave holographic superconductors. On the gravity side, we consider the higher curvature Gauss-Bonnet corrections and on the gauge field side, we add a quadratic correction term to the Maxwell Lagrangian. We show that, for this system, one can still obtain an analytical relation between the critical temperature and the charge density. We also calculate the critical exponent and the condensation value both analytically and numerically. We use a variational method, based on the Sturm-Liouville eigenvalue problem for our analytical study, as well as a numerical shooting method in order to compare with our analytical results. For a fixed value of the Gauss-Bonnet parameter, we observe that the critical temperature decreases with increasing the nonlinearity of the gauge field. This implies that the nonlinear correction term to the Maxwell electrodynamics makes the condensation harder. We also study the holographic conductivity of the system and disclose the effects of the Gauss-Bonnet and nonlinear parameters α and b on the superconducting gap. We observe that, for various values of α and b, the real part of the conductivity is proportional to the frequency per temperature, ω/T, as the frequency is large enough. Besides, the conductivity has a minimum in the imaginary part which is shifted toward greater frequency with decreasing temperature. (orig.)

  4. Photopolymer holographic recording material

    Science.gov (United States)

    Lawrence, J. R.; O'Neill, F. T.; Sheridan, J. T.

    Photopolymers are promising materials for use in holography. They have many advantages, such as ease of preparation, and are capable of efficiencies of up to 100%. A disadvantage of these materials is their inability to record high spatial frequency gratings when compared to other materials such as dichromated gelatin and silver halide photographic emulsion. Until recently, the drop off at high spatial frequencies of the material response was not predicted by any of the diffusion based models available. It has recently been proposed that this effect is due to polymer chains growing away from their initiation point and causing a smeared profile to be recorded. This is termed a non-local material response. Simple analytic expressions have been derived using this model and fits to experimental data have allowed values to be estimated for material parameters such as the diffusion coefficient of monomer, the ratio of polymerisation rate to diffusion rate and the distance that the polymer chains spread during holographic recording. The model predicts that the spatial frequency response might be improved by decreasing the mean polymer chain lengths and/or by increasing the mobility of the molecules used in the material. The experimental work carried out to investigate these predictions is reported here. This work involved (a) the changing of the molecular weights of chemical components within the material (dyes and binders) and (b) the addition of a chemical retarder in order to shorten the polymer chains, thereby decreasing the extent of the non-local effect. Although no significant improvement in spatial frequency response was observed the model appears to offer an improved understanding of the operation of the material.

  5. HOMES - Holographic Optical Method for Exoplanet Spectroscopy

    Data.gov (United States)

    National Aeronautics and Space Administration — HOMES (Holographic Optical Method for Exoplanet Spectroscopy) is a space telescope that employs a double dispersion architecture, using a holographic optical element...

  6. HOLIMO II: a digital holographic instrument for ground-based in situ observations of microphysical properties of mixed-phase clouds

    Science.gov (United States)

    Henneberger, J.; Fugal, J. P.; Stetzer, O.; Lohmann, U.

    2013-11-01

    Measurements of the microphysical properties of mixed-phase clouds with high spatial resolution are important to understand the processes inside these clouds. This work describes the design and characterization of the newly developed ground-based field instrument HOLIMO II (HOLographic Imager for Microscopic Objects II). HOLIMO II uses digital in-line holography to in situ image cloud particles in a well-defined sample volume. By an automated algorithm, two-dimensional images of single cloud particles between 6 and 250 μm in diameter are obtained and the size spectrum, the concentration and water content of clouds are calculated. By testing the sizing algorithm with monosized beads a systematic overestimation near the resolution limit was found, which has been used to correct the measurements. Field measurements from the high altitude research station Jungfraujoch, Switzerland, are presented. The measured number size distributions are in good agreement with parallel measurements by a fog monitor (FM-100, DMT, Boulder USA). The field data shows that HOLIMO II is capable of measuring the number size distribution with a high spatial resolution and determines ice crystal shape, thus providing a method of quantifying variations in microphysical properties. A case study over a period of 8 h has been analyzed, exploring the transition from a liquid to a mixed-phase cloud, which is the longest observation of a cloud with a holographic device. During the measurement period, the cloud does not completely glaciate, contradicting earlier assumptions of the dominance of the Wegener-Bergeron-Findeisen (WBF) process.

  7. HOLIMO II: a digital holographic instrument for ground-based in-situ observations of microphysical properties of mixed-phase clouds

    Science.gov (United States)

    Henneberger, J.; Fugal, J. P.; Stetzer, O.; Lohmann, U.

    2013-05-01

    Measurements of the microphysical properties of mixed-phase clouds with high spatial resolution are important to understand the processes inside these clouds. This work describes the design and characterization of the newly developed ground-based field instrument HOLIMO II (HOLographic Imager for Microscopic Objects II). HOLIMO II uses digital in-line holography to in-situ image cloud particles in a well defined sample volume. By an automated algorithm, two-dimensional images of single cloud particles between 6 and 250 μm in diameter are obtained and the size spectrum, the concentration and water content of clouds are calculated. By testing the sizing algorithm with monosized beads a systematic overestimation near the resolution limit was found, which has been used to correct the measurements. Field measurements from the high altitude research station Jungfraujoch, Switzerland, are presented. The measured number size distributions are in good agreement with parallel measurements by a fog monitor (FM-100, DMT, Boulder USA). The field data shows that HOLIMO II is capable of measuring the number size distribution with a high spatial resolution and determines ice crystal shape, thus providing a method of quantifying variations in microphysical properties. A case study over a period of 8 h has been analyzed, exploring the transition from a liquid to a mixed-phase cloud, which is the longest observation of a cloud with a holographic device. During the measurement period, the cloud does not completely glaciate, contradicting earlier assumptions of the dominance of the Wegener-Bergeron-Findeisen (WBF) process.

  8. Real-time holographic endoscopy

    Science.gov (United States)

    Smigielski, Paul; Albe, Felix; Dischli, Bernard

    1992-08-01

    Some new experiments concerning holographic endoscopy are presented. The quantitative measurements of deformations of objects are obtained by the double-exposure and double- reference beam method, using either a cw-laser or a pulsed laser. Qualitative experiments using an argon laser with time-average holographic endoscopy are also presented. A video film on real-time endoscopic holographic interferometry was recorded with the help of a frequency-doubled YAG-laser working at 25 Hz for the first time.

  9. Intelligent holographic databases

    Science.gov (United States)

    Barbastathis, George

    Memory is a key component of intelligence. In the human brain, physical structure and functionality jointly provide diverse memory modalities at multiple time scales. How could we engineer artificial memories with similar faculties? In this thesis, we attack both hardware and algorithmic aspects of this problem. A good part is devoted to holographic memory architectures, because they meet high capacity and parallelism requirements. We develop and fully characterize shift multiplexing, a novel storage method that simplifies disk head design for holographic disks. We develop and optimize the design of compact refreshable holographic random access memories, showing several ways that 1 Tbit can be stored holographically in volume less than 1 m3, with surface density more than 20 times higher than conventional silicon DRAM integrated circuits. To address the issue of photorefractive volatility, we further develop the two-lambda (dual wavelength) method for shift multiplexing, and combine electrical fixing with angle multiplexing to demonstrate 1,000 multiplexed fixed holograms. Finally, we propose a noise model and an information theoretic metric to optimize the imaging system of a holographic memory, in terms of storage density and error rate. Motivated by the problem of interfacing sensors and memories to a complex system with limited computational resources, we construct a computer game of Desert Survival, built as a high-dimensional non-stationary virtual environment in a competitive setting. The efficacy of episodic learning, implemented as a reinforced Nearest Neighbor scheme, and the probability of winning against a control opponent improve significantly by concentrating the algorithmic effort to the virtual desert neighborhood that emerges as most significant at any time. The generalized computational model combines the autonomous neural network and von Neumann paradigms through a compact, dynamic central representation, which contains the most salient features

  10. Holographic memories with encryption-selectable function

    Science.gov (United States)

    Su, Wei-Chia; Lee, Xuan-Hao

    2006-03-01

    Volume holographic storage has received increasing attention owing to its potential high storage capacity and access rate. In the meanwhile, encrypted holographic memory using random phase encoding technique is attractive for an optical community due to growing demand for protection of information. In this paper, encryption-selectable holographic storage algorithms in LiNbO 3 using angular multiplexing are proposed and demonstrated. Encryption-selectable holographic memory is an advance concept of security storage for content protection. It offers more flexibility to encrypt the data or not optionally during the recording processes. In our system design, the function of encryption and non-encryption storage is switched by a random phase pattern and a uniform phase pattern. Based on a 90-degree geometry, the input patterns including the encryption and non-encryption storage are stored via angular multiplexing with reference plane waves at different incident angles. Image is encrypted optionally by sliding the ground glass into one of the recording waves or removing it away in each exposure. The ground glass is a key for encryption. Besides, it is also an important key available for authorized user to decrypt the encrypted information.

  11. Pattern recognition with magnonic holographic memory device

    International Nuclear Information System (INIS)

    Kozhevnikov, A.; Dudko, G.; Filimonov, Y.; Gertz, F.; Khitun, A.

    2015-01-01

    In this work, we present experimental data demonstrating the possibility of using magnonic holographic devices for pattern recognition. The prototype eight-terminal device consists of a magnetic matrix with micro-antennas placed on the periphery of the matrix to excite and detect spin waves. The principle of operation is based on the effect of spin wave interference, which is similar to the operation of optical holographic devices. Input information is encoded in the phases of the spin waves generated on the edges of the magnonic matrix, while the output corresponds to the amplitude of the inductive voltage produced by the interfering spin waves on the other side of the matrix. The level of the output voltage depends on the combination of the input phases as well as on the internal structure of the magnonic matrix. Experimental data collected for several magnonic matrixes show the unique output signatures in which maxima and minima correspond to specific input phase patterns. Potentially, magnonic holographic devices may provide a higher storage density compare to optical counterparts due to a shorter wavelength and compatibility with conventional electronic devices. The challenges and shortcoming of the magnonic holographic devices are also discussed

  12. Recent advances in photorefractivity of poly(4-diphenylaminostyrene) composites: Wavelength dependence and dynamic holographic images

    Science.gov (United States)

    Tsujimura, Sho; Kinashi, Kenji; Sakai, Wataru; Tsutsumi, Naoto

    2014-08-01

    To expand upon our previous report [Appl. Phys. Express 5, 064101 (2012) 064101], we provide here the modified poly(4-diphenylaminostyrene) (PDAS)-based photorefractive (PR) device on the basis of wavelength dependency, and demonstrate dynamic holographic images by using the PDAS-based PR device under the obtained appropriate conditions. The PR devices containing the triphenylamine unit have potential application to dynamic holographic images, which will be useful for real-time holographic displays.

  13. Deriving covariant holographic entanglement

    Energy Technology Data Exchange (ETDEWEB)

    Dong, Xi [School of Natural Sciences, Institute for Advanced Study, Princeton, NJ 08540 (United States); Lewkowycz, Aitor [Jadwin Hall, Princeton University, Princeton, NJ 08544 (United States); Rangamani, Mukund [Center for Quantum Mathematics and Physics (QMAP), Department of Physics, University of California, Davis, CA 95616 (United States)

    2016-11-07

    We provide a gravitational argument in favour of the covariant holographic entanglement entropy proposal. In general time-dependent states, the proposal asserts that the entanglement entropy of a region in the boundary field theory is given by a quarter of the area of a bulk extremal surface in Planck units. The main element of our discussion is an implementation of an appropriate Schwinger-Keldysh contour to obtain the reduced density matrix (and its powers) of a given region, as is relevant for the replica construction. We map this contour into the bulk gravitational theory, and argue that the saddle point solutions of these replica geometries lead to a consistent prescription for computing the field theory Rényi entropies. In the limiting case where the replica index is taken to unity, a local analysis suffices to show that these saddles lead to the extremal surfaces of interest. We also comment on various properties of holographic entanglement that follow from this construction.

  14. Holographic Raman lidar

    International Nuclear Information System (INIS)

    Andersen, G.

    2000-01-01

    Full text: We have constructed a Raman lidar system that incorporates a holographic optical element. By resolving just 3 nitrogen lines in the Resonance Raman spectroscopy (RRS) spectrum, temperature fits as good as 1% at altitudes of 20km can be made in 30 minutes. Due to the narrowband selectivity of the HOE, the lidar provides measurements over a continuous 24hr period. By adding a 4th channel to capture the Rayleigh backscattered light, temperature profiles can be extended to 80km

  15. Holographic dark energy and f(R) gravity

    Energy Technology Data Exchange (ETDEWEB)

    Aghamohammadi, A [Faculty of Science, Islamic Azad University of Sanandaj, Sanandaj (Iran, Islamic Republic of); Saaidi, Kh, E-mail: ksaaidi@uok.ac.ir, E-mail: agha35484@yahoo.com [Department of Physics, Faculty of Science, University of Kurdistan, Sanandaj (Iran, Islamic Republic of)

    2011-02-15

    We investigate the corresponding relation between f(R) gravity and holographic dark energy. We introduce a type of energy density from f(R) that has the same role as holographic dark energy. We obtain the differential equation that specifies the evolution of the introduced energy density parameter based on a varying gravitational constant. We discover the relation for the equation of state parameter for low redshifts that contains varying G correction.

  16. Review of Random Phase Encoding in Volume Holographic Storage

    Directory of Open Access Journals (Sweden)

    Wei-Chia Su

    2012-09-01

    Full Text Available Random phase encoding is a unique technique for volume hologram which can be applied to various applications such as holographic multiplexing storage, image encryption, and optical sensing. In this review article, we first review and discuss diffraction selectivity of random phase encoding in volume holograms, which is the most important parameter related to multiplexing capacity of volume holographic storage. We then review an image encryption system based on random phase encoding. The alignment of phase key for decryption of the encoded image stored in holographic memory is analyzed and discussed. In the latter part of the review, an all-optical sensing system implemented by random phase encoding and holographic interconnection is presented.

  17. High quality digital holographic reconstruction on analog film

    Science.gov (United States)

    Nelsen, B.; Hartmann, P.

    2017-05-01

    High quality real-time digital holographic reconstruction, i.e. at 30 Hz frame rates, has been at the forefront of research and has been hailed as the holy grail of display systems. While these efforts have produced a fascinating array of computer algorithms and technology, many applications of reconstructing high quality digital holograms do not require such high frame rates. In fact, applications such as 3D holographic lithography even require a stationary mask. Typical devices used for digital hologram reconstruction are based on spatial-light-modulator technology and this technology is great for reconstructing arbitrary holograms on the fly; however, it lacks the high spatial resolution achievable by its analog counterpart, holographic film. Analog holographic film is therefore the method of choice for reconstructing highquality static holograms. The challenge lies in taking a static, high-quality digitally calculated hologram and effectively writing it to holographic film. We have developed a theoretical system based on a tunable phase plate, an intensity adjustable high-coherence laser and a slip-stick based piezo rotation stage to effectively produce a digitally calculated hologram on analog film. The configuration reproduces the individual components, both the amplitude and phase, of the hologram in the Fourier domain. These Fourier components are then individually written on the holographic film after interfering with a reference beam. The system is analogous to writing angularly multiplexed plane waves with individual component phase control.

  18. Inquiry-Based Instruction of Compound Microscopy Using Simulated Paleobiogeography

    Science.gov (United States)

    Hodgson, Jay Y. S.; Mateer, Scott C.

    2015-01-01

    The compound microscope is an important tool in biology, and mastering it requires repetition. Unfortunately, introductory activities for students can be formulaic, and consequently, students are often unengaged and fail to develop the required experience to become proficient in microscopy. To engage students, increase repetition, and develop…

  19. DNA origami-based standards for quantitative fluorescence microscopy.

    Science.gov (United States)

    Schmied, Jürgen J; Raab, Mario; Forthmann, Carsten; Pibiri, Enrico; Wünsch, Bettina; Dammeyer, Thorben; Tinnefeld, Philip

    2014-01-01

    Validating and testing a fluorescence microscope or a microscopy method requires defined samples that can be used as standards. DNA origami is a new tool that provides a framework to place defined numbers of small molecules such as fluorescent dyes or proteins in a programmed geometry with nanometer precision. The flexibility and versatility in the design of DNA origami microscopy standards makes them ideally suited for the broad variety of emerging super-resolution microscopy methods. As DNA origami structures are durable and portable, they can become a universally available specimen to check the everyday functionality of a microscope. The standards are immobilized on a glass slide, and they can be imaged without further preparation and can be stored for up to 6 months. We describe a detailed protocol for the design, production and use of DNA origami microscopy standards, and we introduce a DNA origami rectangle, bundles and a nanopillar as fluorescent nanoscopic rulers. The protocol provides procedures for the design and realization of fluorescent marks on DNA origami structures, their production and purification, quality control, handling, immobilization, measurement and data analysis. The procedure can be completed in 1-2 d.

  20. Cytology 3D structure formation based on optical microscopy images

    Science.gov (United States)

    Pronichev, A. N.; Polyakov, E. V.; Shabalova, I. P.; Djangirova, T. V.; Zaitsev, S. M.

    2017-01-01

    The article the article is devoted to optimization of the parameters of imaging of biological preparations in optical microscopy using a multispectral camera in visible range of electromagnetic radiation. A model for the image forming of virtual preparations was proposed. The optimum number of layers was determined for the object scan in depth and holistic perception of its switching according to the results of the experiment.

  1. Cytology 3D structure formation based on optical microscopy images

    International Nuclear Information System (INIS)

    Pronichev, A N; Polyakov, E V; Zaitsev, S M; Shabalova, I P; Djangirova, T V

    2017-01-01

    The article the article is devoted to optimization of the parameters of imaging of biological preparations in optical microscopy using a multispectral camera in visible range of electromagnetic radiation. A model for the image forming of virtual preparations was proposed. The optimum number of layers was determined for the object scan in depth and holistic perception of its switching according to the results of the experiment. (paper)

  2. A holographic bound for D3-brane

    Energy Technology Data Exchange (ETDEWEB)

    Momeni, Davood; Myrzakul, Aizhan; Myrzakulov, Ratbay [Eurasian National University, Eurasian International Center for Theoretical Physics, Astana (Kazakhstan); Eurasian National University, Department of General Theoretical Physics, Astana (Kazakhstan); Faizal, Mir [University of British Columbia-Okanagan, Irving K. Barber School of Arts and Sciences, Kelowna, BC (Canada); University of Lethbridge, Department of Physics and Astronomy, Lethbridge, AB (Canada); Bahamonde, Sebastian [University College London, Department of Mathematics, London (United Kingdom)

    2017-06-15

    In this paper, we will regularize the holographic entanglement entropy, holographic complexity and fidelity susceptibility for a configuration of D3-branes. We will also study the regularization of the holographic complexity from the action for a configuration of D3-branes. It will be demonstrated that for a spherical shell of D3-branes the regularized holographic complexity is always greater than or equal to the regularized fidelity susceptibility. Furthermore, we will also demonstrate that the regularized holographic complexity is related to the regularized holographic entanglement entropy for this system. Thus, we will obtain a holographic bound involving regularized holographic complexity, regularized holographic entanglement entropy and regularized fidelity susceptibility of a configuration of D3-brane. We will also discuss a bound for regularized holographic complexity from action, for a D3-brane configuration. (orig.)

  3. The Hubble IR cutoff in holographic ellipsoidal cosmologies

    Energy Technology Data Exchange (ETDEWEB)

    Cataldo, Mauricio [Universidad del Bio-Bio, Departamento de Fisica, Facultad de Ciencias, Concepcion (Chile); Cruz, Norman [Grupo de Cosmologia y Gravitacion-UBB, Concepcion (Chile)

    2018-01-15

    It is well known that for spatially flat FRW cosmologies, the holographic dark energy disfavors the Hubble parameter as a candidate for the IR cutoff. For overcoming this problem, we explore the use of this cutoff in holographic ellipsoidal cosmological models, and derive the general ellipsoidal metric induced by a such holographic energy density. Despite the drawbacks that this cutoff presents in homogeneous and isotropic universes, based on this general metric, we developed a suitable ellipsoidal holographic cosmological model, filled with a dark matter and a dark energy components. At late time stages, the cosmic evolution is dominated by a holographic anisotropic dark energy with barotropic equations of state. The cosmologies expand in all directions in accelerated manner. Since the ellipsoidal cosmologies given here are not asymptotically FRW, the deviation from homogeneity and isotropy of the universe on large cosmological scales remains constant during all cosmic evolution. This feature allows the studied holographic ellipsoidal cosmologies to be ruled by an equation of state ω = p/ρ, whose range belongs to quintessence or even phantom matter. (orig.)

  4. Holographic effective field theories

    Energy Technology Data Exchange (ETDEWEB)

    Martucci, Luca [Dipartimento di Fisica ed Astronomia “Galileo Galilei' , Università di Padova,and INFN - Sezione di Padova, Via Marzolo 8, I-35131 Padova (Italy); Zaffaroni, Alberto [Dipartimento di Fisica, Università di Milano-Bicocca,and INFN - Sezione di Milano-Bicocca, I-20126 Milano (Italy)

    2016-06-28

    We derive the four-dimensional low-energy effective field theory governing the moduli space of strongly coupled superconformal quiver gauge theories associated with D3-branes at Calabi-Yau conical singularities in the holographic regime of validity. We use the dual supergravity description provided by warped resolved conical geometries with mobile D3-branes. Information on the baryonic directions of the moduli space is also obtained by using wrapped Euclidean D3-branes. We illustrate our general results by discussing in detail their application to the Klebanov-Witten model.

  5. Holographic twin Higgs model.

    Science.gov (United States)

    Geller, Michael; Telem, Ofri

    2015-05-15

    We present the first realization of a "twin Higgs" model as a holographic composite Higgs model. Uniquely among composite Higgs models, the Higgs potential is protected by a new standard model (SM) singlet elementary "mirror" sector at the sigma model scale f and not by the composite states at m_{KK}, naturally allowing for m_{KK} beyond the LHC reach. As a result, naturalness in our model cannot be constrained by the LHC, but may be probed by precision Higgs measurements at future lepton colliders, and by direct searches for Kaluza-Klein excitations at a 100 TeV collider.

  6. Holographic Twin Higgs Model

    Science.gov (United States)

    Geller, Michael; Telem, Ofri

    2015-05-01

    We present the first realization of a "twin Higgs" model as a holographic composite Higgs model. Uniquely among composite Higgs models, the Higgs potential is protected by a new standard model (SM) singlet elementary "mirror" sector at the sigma model scale f and not by the composite states at mKK , naturally allowing for mKK beyond the LHC reach. As a result, naturalness in our model cannot be constrained by the LHC, but may be probed by precision Higgs measurements at future lepton colliders, and by direct searches for Kaluza-Klein excitations at a 100 TeV collider.

  7. Volume holographic memory

    Directory of Open Access Journals (Sweden)

    Cornelia Denz

    2000-05-01

    Full Text Available Volume holography represents a promising alternative to existing storage technologies. Its parallel data storage leads to high capacities combined with short access times and high transfer rates. The design and realization of a compact volume holographic storage demonstrator is presented. The technique of phase-coded multiplexing implemented to superimpose many data pages in a single location enables to store up to 480 holograms per storage location without any moving parts. Results of analog and digital data storage are shown and real time optical image processing is demonstrated.

  8. Holographic magnetisation density waves

    Energy Technology Data Exchange (ETDEWEB)

    Donos, Aristomenis [Centre for Particle Theory and Department of Mathematical Sciences, Durham University,Stockton Road, Durham, DH1 3LE (United Kingdom); Pantelidou, Christiana [Departament de Fisica Quantica i Astrofisica & Institut de Ciencies del Cosmos (ICC),Universitat de Barcelona,Marti i Franques 1, 08028 Barcelona (Spain)

    2016-10-10

    We numerically construct asymptotically AdS black brane solutions of D=4 Einstein theory coupled to a scalar and two U(1) gauge fields. The solutions are holographically dual to d=3 CFTs in a constant external magnetic field along one of the U(1)’s. Below a critical temperature the system’s magnetisation density becomes inhomogeneous, leading to spontaneous formation of current density waves. We find that the transition can be of second order and that the solutions which minimise the free energy locally in the parameter space of solutions have averaged stressed tensor of a perfect fluid.

  9. Phases of Holographic QCD

    International Nuclear Information System (INIS)

    Lippert, Matthew

    2009-01-01

    We investigated the Sakai-Sugimoto model of large N QCD at nonzero temperature and baryon chemical potential and in the presence of background electric and magnetic fields. We studied the holographic representation of baryons and the deconfinement, chiral-symmetry breaking, and nuclear matter phase transitions. In a background electric field, chiral-symmetry breaking corresponds to an insulator-conductor transition. A magnetic field both catalyzes chiral-symmetry breaking and generates, in the confined phase, a pseudo-scalar gradient or, in the deconfined phase, an axial current. The resulting phase diagram is in qualitative agreement with studies of hot, dense QCD.

  10. X-ray diffraction microscopy based on refractive optics

    DEFF Research Database (Denmark)

    Poulsen, Henning Friis; Jakobsen, A. C.; Simons, Hugh

    2017-01-01

    A formalism is presented for dark‐field X‐ray microscopy using refractive optics. The new technique can produce three‐dimensional maps of lattice orientation and axial strain within millimetre‐sized sampling volumes and is particularly suited to in situ studies of materials at hard X‐ray energies....... An objective lens in the diffracted beam magnifies the image and acts as a very efficient filter in reciprocal space, enabling the imaging of individual domains of interest with a resolution of 100 nm. Analytical expressions for optical parameters such as numerical aperture, vignetting, and the resolution...

  11. Mobile phone based clinical microscopy for global health applications.

    Directory of Open Access Journals (Sweden)

    David N Breslauer

    Full Text Available Light microscopy provides a simple, cost-effective, and vital method for the diagnosis and screening of hematologic and infectious diseases. In many regions of the world, however, the required equipment is either unavailable or insufficiently portable, and operators may not possess adequate training to make full use of the images obtained. Counterintuitively, these same regions are often well served by mobile phone networks, suggesting the possibility of leveraging portable, camera-enabled mobile phones for diagnostic imaging and telemedicine. Toward this end we have built a mobile phone-mounted light microscope and demonstrated its potential for clinical use by imaging P. falciparum-infected and sickle red blood cells in brightfield and M. tuberculosis-infected sputum samples in fluorescence with LED excitation. In all cases resolution exceeded that necessary to detect blood cell and microorganism morphology, and with the tuberculosis samples we took further advantage of the digitized images to demonstrate automated bacillus counting via image analysis software. We expect such a telemedicine system for global healthcare via mobile phone -- offering inexpensive brightfield and fluorescence microscopy integrated with automated image analysis -- to provide an important tool for disease diagnosis and screening, particularly in the developing world and rural areas where laboratory facilities are scarce but mobile phone infrastructure is extensive.

  12. Shrinkage measurement for holographic recording materials

    Science.gov (United States)

    Fernández, R.; Gallego, S.; Márquez, A.; Francés, J.; Navarro Fuster, V.; Neipp, C.; Ortuño, M.; Beléndez, A.; Pascual, I.

    2017-05-01

    There is an increasing demand for new holographic recording materials. One of them are photopolymers, which are becoming a classic media in this field. Their versatility is well known and new possibilities are being created by including new components, such as nanoparticles or dispersed liquid crystal molecules in classical formulations, making them interesting for additional applications in which the thin film preparation and the structural modification have a fundamental importance. Prior to obtaining a wide commercialization of displays based on photopolymers, one of the key aspects is to achieve a complete characterization of them. In this sense, one of the main parameters to estimate and control is the shrinkage of these materials. The volume variations change the angular response of the hologram in two aspects, the angular selectivity and the maximum diffraction efficiency. One criteria for the recording material to be used in a holographic data storage application is the shrinkage, maximum of 0.5%. Along this work, we compare two different methods to measure the holographic recording material shrinkage. The first one is measuring the angle of propagation for both diffracted orders +/-1 when slanted gratings are recorded, so that an accurate value of the grating vector can be calculated. The second one is based on interference measurements at zero spatial frequency limit. We calculate the shrinkage for three different photopolymers: a polyvinyl alcohol acrylamide (PVA/AA) based photopolymer, one of the greenest photopolymers whose patent belongs to the Alicante University called Biophotopol and on the last place a holographic-dispersed liquid crystal photopolymer (H-PDLC).

  13. Holographic models and the QCD trace anomaly

    International Nuclear Information System (INIS)

    Goity, Jose L.; Trinchero, Roberto C.

    2012-01-01

    Five dimensional dilaton models are considered as possible holographic duals of the pure gauge QCD vacuum. In the framework of these models, the QCD trace anomaly equation is considered. Each quantity appearing in that equation is computed by holographic means. Two exact solutions for different dilaton potentials corresponding to perturbative and non-perturbative β-functions are studied. It is shown that in the perturbative case, where the β-function is the QCD one at leading order, the resulting space is not asymptotically AdS. In the non-perturbative case, the model considered presents confinement of static quarks and leads to a non-vanishing gluon condensate, although it does not correspond to an asymptotically free theory. In both cases analyses based on the trace anomaly and on Wilson loops are carried out.

  14. Towards understanding Regge trajectories in holographic QCD

    International Nuclear Information System (INIS)

    Cata, Oscar

    2007-01-01

    We reassess a work done by Migdal on the spectrum of low-energy vector mesons in QCD in the light of the anti-de Sitter (AdS)-QCD correspondence. Recently, a tantalizing parallelism was suggested between Migdal's work and a family of holographic duals of QCD. Despite the intriguing similarities, both approaches face a major drawback: the spectrum is in conflict with well-tested Regge scaling. However, it has recently been shown that holographic duals can be modified to accommodate Regge behavior. Therefore, it is interesting to understand whether Regge behavior can also be achieved in Migdal's approach. In this paper we investigate this issue. We find that Migdal's approach, which is based on a modified Pade approximant, is closely related to the issue of quark-hadron duality breakdown in QCD

  15. The traveltime holographic principle

    KAUST Repository

    Huang, Y.; Schuster, Gerard T.

    2014-01-01

    Fermat's interferometric principle is used to compute interior transmission traveltimes τpq from exterior transmission traveltimes τsp and τsq. Here, the exterior traveltimes are computed for sources s on a boundary B that encloses a volume V of interior points p and q. Once the exterior traveltimes are computed, no further ray tracing is needed to calculate the interior times τpq. Therefore this interferometric approach can be more efficient than explicitly computing interior traveltimes τpq by ray tracing. Moreover, the memory requirement of the traveltimes is reduced by one dimension, because the boundary B is of one fewer dimension than the volume V. An application of this approach is demonstrated with interbed multiple (IM) elimination. Here, the IMs in the observed data are predicted from the migration image and are subsequently removed by adaptive subtraction. This prediction is enabled by the knowledge of interior transmission traveltimes τpq computed according to Fermat's interferometric principle. We denote this principle as the ‘traveltime holographic principle’, by analogy with the holographic principle in cosmology where information in a volume is encoded on the region's boundary.

  16. The traveltime holographic principle

    KAUST Repository

    Huang, Y.

    2014-11-06

    Fermat\\'s interferometric principle is used to compute interior transmission traveltimes τpq from exterior transmission traveltimes τsp and τsq. Here, the exterior traveltimes are computed for sources s on a boundary B that encloses a volume V of interior points p and q. Once the exterior traveltimes are computed, no further ray tracing is needed to calculate the interior times τpq. Therefore this interferometric approach can be more efficient than explicitly computing interior traveltimes τpq by ray tracing. Moreover, the memory requirement of the traveltimes is reduced by one dimension, because the boundary B is of one fewer dimension than the volume V. An application of this approach is demonstrated with interbed multiple (IM) elimination. Here, the IMs in the observed data are predicted from the migration image and are subsequently removed by adaptive subtraction. This prediction is enabled by the knowledge of interior transmission traveltimes τpq computed according to Fermat\\'s interferometric principle. We denote this principle as the ‘traveltime holographic principle’, by analogy with the holographic principle in cosmology where information in a volume is encoded on the region\\'s boundary.

  17. The traveltime holographic principle

    Science.gov (United States)

    Huang, Yunsong; Schuster, Gerard T.

    2015-01-01

    Fermat's interferometric principle is used to compute interior transmission traveltimes τpq from exterior transmission traveltimes τsp and τsq. Here, the exterior traveltimes are computed for sources s on a boundary B that encloses a volume V of interior points p and q. Once the exterior traveltimes are computed, no further ray tracing is needed to calculate the interior times τpq. Therefore this interferometric approach can be more efficient than explicitly computing interior traveltimes τpq by ray tracing. Moreover, the memory requirement of the traveltimes is reduced by one dimension, because the boundary B is of one fewer dimension than the volume V. An application of this approach is demonstrated with interbed multiple (IM) elimination. Here, the IMs in the observed data are predicted from the migration image and are subsequently removed by adaptive subtraction. This prediction is enabled by the knowledge of interior transmission traveltimes τpq computed according to Fermat's interferometric principle. We denote this principle as the `traveltime holographic principle', by analogy with the holographic principle in cosmology where information in a volume is encoded on the region's boundary.

  18. Image improvement and three-dimensional reconstruction using holographic image processing

    Science.gov (United States)

    Stroke, G. W.; Halioua, M.; Thon, F.; Willasch, D. H.

    1977-01-01

    Holographic computing principles make possible image improvement and synthesis in many cases of current scientific and engineering interest. Examples are given for the improvement of resolution in electron microscopy and 3-D reconstruction in electron microscopy and X-ray crystallography, following an analysis of optical versus digital computing in such applications.

  19. Drawing Lines with Light in Holographic Space

    International Nuclear Information System (INIS)

    Chang, Yin-Ren; Richardson, Martin

    2013-01-01

    This paper explores the dynamic and expressive possibilities of holographic art through a comparison of art history and technical media such as photography, film and holographic technologies. Examples of modern art and creative expression of time and motions are examined using the early 20th century art movement, Cubism, where subjects are portrayed to be seen simultaneously from different angles. Folding space is represented as subject matter as it can depict space from multiple points of time. The paper also investigates the way holographic art has explored time and space. The lenticular lens-based media reveal a more subjective poetic art in the form of the lyrical images and messages as spectators pass through time, or walk along with the piece of work through an interactive process. It is argued that photographic practice is another example of artistic representation in the form of aesthetic medium of time movement and as such shares a common ground with other dynamic expression that require time based interaction.

  20. Holographic sensors for diagnostics of solution components

    International Nuclear Information System (INIS)

    Kraiskii, A V; Suitanov, T T; Postnikov, V A; Khamidulin, A V

    2010-01-01

    The properties of holographic sensors of two types are studied. The sensors are based on a three-dimensional polymer-network matrix of copolymers of acrylamide, acrylic acid (which are sensitive to the medium acidity and bivalent metal ions) and aminophenylboronic acid (sensitive to glucose). It is found that a change in the ionic composition of a solution results in changes in the distance between layers and in the diffraction efficiency of holograms. Variations in the shape of spectral lines, which are attributed to the inhomogeneity of a sensitive layer, and nonmonotonic changes in the emulsion thickness and diffraction efficiency were observed during transient processes. The composition of the components of a hydrogel medium is selected for systems which can be used as a base for glucose sensors with the mean holographic response in the region of physiological glucose concentration in model solutions achieving 40 nm/(mmol L -1 ). It is shown that the developed holographic sensors can be used for the visual and instrumental determination of the medium acidity, alcohol content, ionic strength, bivalent metal salts and the quality of water, in particular, for drinking. (laser applications and other topics in quantum electronics)

  1. Holographic Jet Quenching

    Science.gov (United States)

    Ficnar, Andrej

    In this dissertation we study the phenomenon of jet quenching in quark-gluon plasma using the AdS/CFT correspondence. We start with a weakly coupled, perturbative QCD approach to energy loss, and present a Monte Carlo code for computation of the DGLV radiative energy loss of quarks and gluons at an arbitrary order in opacity. We use the code to compute the radiated gluon distribution up to n=9 order in opacity, and compare it to the thin plasma (n=1) and the multiple soft scattering (n=infinity) approximations. We furthermore show that the gluon distribution at finite opacity depends in detail on the screening mass mu and the mean free path lambda. In the next part, we turn to the studies of how heavy quarks, represented as "trailing strings" in AdS/CFT, lose energy in a strongly coupled plasma. We study how the heavy quark energy loss gets modified in a "bottom-up" non-conformal holographic model, constructed to reproduce some properties of QCD at finite temperature and constrained by fitting the lattice gauge theory results. The energy loss of heavy quarks is found to be strongly sensitive to the medium properties. We use this model to compute the nuclear modification factor RAA of charm and bottom quarks in an expanding plasma with Glauber initial conditions, and comment on the range of validity of the model. The central part of this thesis is the energy loss of light quarks in a strongly coupled plasma. Using the standard model of "falling strings", we present an analytic derivation of the stopping distance of light quarks, previously available only through numerical simulations, and also apply it to the case of Gauss-Bonnet higher derivative gravity. We then present a general formula for computing the instantaneous energy loss in non-stationary string configurations. Application of this formula to the case of falling strings reveals interesting phenomenology, including a modified Bragg-like peak at late times and an approximately linear path dependence. Based

  2. Adventures in holographic dimer models

    International Nuclear Information System (INIS)

    Kachru, Shamit; Karch, Andreas; Yaida, Sho

    2011-01-01

    We abstract the essential features of holographic dimer models, and develop several new applications of these models. Firstly, semi-holographically coupling free band fermions to holographic dimers, we uncover novel phase transitions between conventional Fermi liquids and non-Fermi liquids, accompanied by a change in the structure of the Fermi surface. Secondly, we make dimer vibrations propagate through the whole crystal by way of double trace deformations, obtaining nontrivial band structure. In a simple toy model, the topology of the band structure experiences an interesting reorganization as we vary the strength of the double trace deformations. Finally, we develop tools that would allow one to build, in a bottom-up fashion, a holographic avatar of the Hubbard model.

  3. Computer assisted holographic moire contouring

    Science.gov (United States)

    Sciammarella, Cesar A.

    2000-01-01

    Theoretical analyses and experimental results on holographic moire contouring on diffusely reflecting objects are presented. The sensitivity and limitations of the method are discussed. Particular emphasis is put on computer-assisted data retrieval, processing, and recording.

  4. Constraining holographic cosmology using Planck data

    Science.gov (United States)

    Afshordi, Niayesh; Gould, Elizabeth; Skenderis, Kostas

    2017-06-01

    Holographic cosmology offers a novel framework for describing the very early Universe in which cosmological predictions are expressed in terms of the observables of a three-dimensional quantum field theory (QFT). This framework includes conventional slow-roll inflation, which is described in terms of a strongly coupled QFT, but it also allows for qualitatively new models for the very early Universe, where the dual QFT may be weakly coupled. The new models describe a universe which is nongeometric at early times. While standard slow-roll inflation leads to a (near-) power-law primordial power spectrum, perturbative super-renormalizable QFTs yield a new holographic spectral shape. Here, we compare the two predictions against cosmological observations. We use CosmoMC to determine the best fit parameters, and MultiNest for Bayesian evidence, comparing the likelihoods. We find that the dual QFT should be nonperturbative at the very low multipoles (l ≲30 ), while for higher multipoles (l ≳30 ) the new holographic model, based on perturbative QFT, fits the data just as well as the standard power-law spectrum assumed in Λ CDM cosmology. This finding opens the door to applications of nonperturbative QFT techniques, such as lattice simulations, to observational cosmology on gigaparsec scales and beyond.

  5. Holographic complexity and noncommutative gauge theory

    Science.gov (United States)

    Couch, Josiah; Eccles, Stefan; Fischler, Willy; Xiao, Ming-Lei

    2018-03-01

    We study the holographic complexity of noncommutative field theories. The four-dimensional N=4 noncommutative super Yang-Mills theory with Moyal algebra along two of the spatial directions has a well known holographic dual as a type IIB supergravity theory with a stack of D3 branes and non-trivial NS-NS B fields. We start from this example and find that the late time holographic complexity growth rate, based on the "complexity equals action" conjecture, experiences an enhancement when the non-commutativity is turned on. This enhancement saturates a new limit which is exactly 1/4 larger than the commutative value. We then attempt to give a quantum mechanics explanation of the enhancement. Finite time behavior of the complexity growth rate is also studied. Inspired by the non-trivial result, we move on to more general setup in string theory where we have a stack of D p branes and also turn on the B field. Multiple noncommutative directions are considered in higher p cases.

  6. Mach's holographic principle

    International Nuclear Information System (INIS)

    Khoury, Justin; Parikh, Maulik

    2009-01-01

    Mach's principle is the proposition that inertial frames are determined by matter. We put forth and implement a precise correspondence between matter and geometry that realizes Mach's principle. Einstein's equations are not modified and no selection principle is applied to their solutions; Mach's principle is realized wholly within Einstein's general theory of relativity. The key insight is the observation that, in addition to bulk matter, one can also add boundary matter. Given a space-time, and thus the inertial frames, we can read off both boundary and bulk stress tensors, thereby relating matter and geometry. We consider some global conditions that are necessary for the space-time to be reconstructible, in principle, from bulk and boundary matter. Our framework is similar to that of the black hole membrane paradigm and, in asymptotically anti-de Sitter space-times, is consistent with holographic duality.

  7. On holographic defect entropy

    International Nuclear Information System (INIS)

    Estes, John; Jensen, Kristan; O’Bannon, Andy; Tsatis, Efstratios; Wrase, Timm

    2014-01-01

    We study a number of (3+1)- and (2+1)-dimensional defect and boundary conformal field theories holographically dual to supergravity theories. In all cases the defects or boundaries are planar, and the defects are codimension-one. Using holography, we compute the entanglement entropy of a (hemi-)spherical region centered on the defect (boundary). We define defect and boundary entropies from the entanglement entropy by an appropriate background subtraction. For some (3+1)-dimensional theories we find evidence that the defect/boundary entropy changes monotonically under certain renormalization group flows triggered by operators localized at the defect or boundary. This provides evidence that the g-theorem of (1+1)-dimensional field theories generalizes to higher dimensions

  8. Holographic Chiral Magnetic Spiral

    International Nuclear Information System (INIS)

    Kim, Keun-Young; Sahoo, Bindusar; Yee, Ho-Ung

    2010-06-01

    We study the ground state of baryonic/axial matter at zero temperature chiral-symmetry broken phase under a large magnetic field, in the framework of holographic QCD by Sakai-Sugimoto. Our study is motivated by a recent proposal of chiral magnetic spiral phase that has been argued to be favored against previously studied phase of homogeneous distribution of axial/baryonic currents in terms of meson super-currents dictated by triangle anomalies in QCD. Our results provide an existence proof of chiral magnetic spiral in strong coupling regime via holography, at least for large axial chemical potentials, whereas we don't find the phenomenon in the case of purely baryonic chemical potential. (author)

  9. Compact Holographic Data Storage

    Science.gov (United States)

    Chao, T. H.; Reyes, G. F.; Zhou, H.

    2001-01-01

    NASA's future missions would require massive high-speed onboard data storage capability to Space Science missions. For Space Science, such as the Europa Lander mission, the onboard data storage requirements would be focused on maximizing the spacecraft's ability to survive fault conditions (i.e., no loss in stored science data when spacecraft enters the 'safe mode') and autonomously recover from them during NASA's long-life and deep space missions. This would require the development of non-volatile memory. In order to survive in the stringent environment during space exploration missions, onboard memory requirements would also include: (1) survive a high radiation environment (1 Mrad), (2) operate effectively and efficiently for a very long time (10 years), and (3) sustain at least a billion write cycles. Therefore, memory technologies requirements of NASA's Earth Science and Space Science missions are large capacity, non-volatility, high-transfer rate, high radiation resistance, high storage density, and high power efficiency. JPL, under current sponsorship from NASA Space Science and Earth Science Programs, is developing a high-density, nonvolatile and rad-hard Compact Holographic Data Storage (CHDS) system to enable large-capacity, high-speed, low power consumption, and read/write of data in a space environment. The entire read/write operation will be controlled with electrooptic mechanism without any moving parts. This CHDS will consist of laser diodes, photorefractive crystal, spatial light modulator, photodetector array, and I/O electronic interface. In operation, pages of information would be recorded and retrieved with random access and high-speed. The nonvolatile, rad-hard characteristics of the holographic memory will provide a revolutionary memory technology meeting the high radiation challenge facing the Europa Lander mission. Additional information is contained in the original extended abstract.

  10. Holographic diffuser by use of a silver halide sensitized gelatin process

    Science.gov (United States)

    Kim, Sun Il; Choi, Yoon Sun; Ham, Yong Nam; Park, Chong Yun; Kim, Jong Man

    2003-05-01

    Diffusers play an important role in liquid-crystal display (LCD) application as a beam-shaping device, a brightness homogenizer, a light-scattering device, and an imaging screen. The transmittance and diffusing angle of the diffusers are the critical aspects for the applications to the LCD. The holographic diffusers by use of various processing methods have been investigated. The diffusing characteristics of different diffusing materials and processing methods have been evaluated and compared. The micro-structures of holographic diffusers have been investigated by use of using scanning electron microscopy. The holographic diffusers by use of the silver halide sensitized gelatin (SHSG) method have the structural merits for the improvement of the quality of diffusers. The features of holographic diffuser were exceptional in terms of transmittance and diffusing angle. The replication method by use of the SHSG process can be directly used for the manufacturing of diffusers for the display application.

  11. QUALITY ASSESSMENT OF CONFOCAL MICROSCOPY SLIDE-BASED SYSTEMS: INSTABLITY

    Science.gov (United States)

    Background: All slide-based fluorescence cytometry detections systems basically include an excitation light source, intermediate optics, and a detection device (CCD or PMT). Occasionally, this equipment becomes unstable, generating unreliable and inferior data. Methods: A num...

  12. Holographic patterning of organic-inorganic photopolymerizable nanocomposites

    Science.gov (United States)

    Sakhno, Oksana V.; Goldenberg, Leonid M.; Smirnova, Tatiana N.; Stumpe, J.

    2009-09-01

    We present here novel easily processible organic-inorganic nanocomposites suitable for holographic fabrication of diffraction optical elements (DOE). The nanocomposites are based on photocurable acrylate monomers and inorganic nanoparticles (NP). The compatibility of inorganic NP with monomers was achieved by capping the NP surface with proper organic shells. Surface modification allows to introduce up to 50wt.% of inorganic NP in organic media. Depending on the NP nature (metal oxides, phosphates, semiconductors, noble metals) and their properties, the materials for both efficient DOE and multifunctional elements can be designed. Organic-inorganic composites prepared have been successfully used for the effective inscription of periodic volume refractive index structures using the holographic photopolymerization method. The nanocomposite preparation procedure, their properties and optical performance of holographic gratings are reported. The use of functional NP makes it possible to obtain effective holographic gratings having additional physical properties such as light-emission or NLO. Some examples of such functional polymer-NP structures and their possible application fields are presented. The combination of easy photo-patterning of soft organic compounds with physical properties of inorganic materials in new nanocomposites and the flexibility of the holographic patterning method allow the fabrication of mono- and multifunctional one- and multi-dimensional passive or active optical and photonic elements.

  13. Holographic data storage: science fiction or science fact?

    Science.gov (United States)

    Anderson, Ken; Ayres, Mark; Askham, Fred; Sissom, Brad

    2014-09-01

    To compete in the archive and backup industries, holographic data storage must be highly competitive in four critical areas: total cost of ownership (TCO), cost/TB, capacity/footprint, and transfer rate. New holographic technology advancements by Akonia Holographics have enabled the potential for ultra-high capacity holographic storage devices that are capable of world record bit densities of over 2-4Tbit/in2, up to 200MB/s transfer rates, and media costs less than $10/TB in the next few years. Additional advantages include more than a 3x lower TCO than LTO, a 3.5x decrease in volumetric footprint, 30ms random access times, and 50 year archive life. At these bit densities, 4.5 Petabytes of uncompressed user data could be stored in a 19" rack system. A demonstration platform based on these new advances has been designed and built by Akonia to progressively demonstrate bit densities of 2Tb/in2, 4Tb/in2, and 8Tb/in2 over the next year. Keywords: holographic

  14. Silicon Nano fabrication by Atomic Force Microscopy-Based Mechanical Processing

    International Nuclear Information System (INIS)

    Miyake, Sh.; Wang, M.; Kim, J.

    2014-01-01

    This paper reviews silicon nano fabrication processes using atomic force microscopy (AFM). In particular, it summarizes recent results obtained in our research group regarding AFM-based silicon nano fabrication through mechanochemical local oxidation by diamond tip sliding, as well as mechanical, electrical, and electromechanical processing using an electrically conductive diamond tip. Microscopic three-dimensional manufacturing mainly relies on etching, deposition, and lithography. Therefore, a special emphasis was placed on nano mechanical processes, mechanochemical reaction by potassium hydroxide solution etching, and mechanical and electrical approaches. Several important surface characterization techniques consisting of scanning tunneling microscopy and related techniques, such as scanning probe microscopy and AFM, were also discussed.

  15. Pupil-segmentation-based adaptive optics for microscopy

    Science.gov (United States)

    Ji, Na; Milkie, Daniel E.; Betzig, Eric

    2011-03-01

    Inhomogeneous optical properties of biological samples make it difficult to obtain diffraction-limited resolution in depth. Correcting the sample-induced optical aberrations needs adaptive optics (AO). However, the direct wavefront-sensing approach commonly used in astronomy is not suitable for most biological samples due to their strong scattering of light. We developed an image-based AO approach that is insensitive to sample scattering. By comparing images of the sample taken with different segments of the pupil illuminated, local tilt in the wavefront is measured from image shift. The aberrated wavefront is then obtained either by measuring the local phase directly using interference or with phase reconstruction algorithms similar to those used in astronomical AO. We implemented this pupil-segmentation-based approach in a two-photon fluorescence microscope and demonstrated that diffraction-limited resolution can be recovered from nonbiological and biological samples.

  16. Holography and holographic dark energy model

    International Nuclear Information System (INIS)

    Gong Yungui; Zhang Yuanzhong

    2005-01-01

    The holographic principle is used to discuss the holographic dark energy model. We find that the Bekenstein-Hawking entropy bound is far from saturation under certain conditions. A more general constraint on the parameter of the holographic dark energy model is also derived

  17. Cellular Dynamics Revealed by Digital Holographic Microscopy☆

    KAUST Repository

    Marquet, P.; Depeursinge, Christian; Jourdain, P.

    2016-01-01

    Digital holographic microscopy (DHM) is a new optical method that provides, without the use of any contrast agent, real-time, three-dimensional images of transparent living cells, with an axial sensitivity of a few tens of nanometers. They result from the hologram numerical reconstruction process, which permits a sub wavelength calculation of the phase shift, produced on the transmitted wave front, by the optically probed cells, namely the quantitative phase signal (QPS). Specifically, in addition to measurements of cellular surface morphometry and intracellular refractive index (RI), various biophysical cellular parameters including dry mass, absolute volume, membrane fluctuations at the nanoscale and biomechanical properties, transmembrane water permeability as swell as current, can be derived from the QPS. This article presents how quantitative phase DHM (QP-DHM) can explored cell dynamics at the nanoscale with a special attention to both the study of neuronal dynamics and the optical resolution of local neuronal network.

  18. Cellular Dynamics Revealed by Digital Holographic Microscopy☆

    KAUST Repository

    Marquet, P.

    2016-11-22

    Digital holographic microscopy (DHM) is a new optical method that provides, without the use of any contrast agent, real-time, three-dimensional images of transparent living cells, with an axial sensitivity of a few tens of nanometers. They result from the hologram numerical reconstruction process, which permits a sub wavelength calculation of the phase shift, produced on the transmitted wave front, by the optically probed cells, namely the quantitative phase signal (QPS). Specifically, in addition to measurements of cellular surface morphometry and intracellular refractive index (RI), various biophysical cellular parameters including dry mass, absolute volume, membrane fluctuations at the nanoscale and biomechanical properties, transmembrane water permeability as swell as current, can be derived from the QPS. This article presents how quantitative phase DHM (QP-DHM) can explored cell dynamics at the nanoscale with a special attention to both the study of neuronal dynamics and the optical resolution of local neuronal network.

  19. All-optical optoacoustic microscopy based on probe beam deflection technique

    OpenAIRE

    Maswadi, Saher M.; Ibey, Bennett L.; Roth, Caleb C.; Tsyboulski, Dmitri A.; Beier, Hope T.; Glickman, Randolph D.; Oraevsky, Alexander A.

    2016-01-01

    Optoacoustic (OA) microscopy using an all-optical system based on the probe beam deflection technique (PBDT) for detection of laser-induced acoustic signals was investigated as an alternative to conventional piezoelectric transducers. PBDT provides a number of advantages for OA microscopy including (i) efficient coupling of laser excitation energy to the samples being imaged through the probing laser beam, (ii) undistorted coupling of acoustic waves to the detector without the need for separa...

  20. Robust holographic storage system design.

    Science.gov (United States)

    Watanabe, Takahiro; Watanabe, Minoru

    2011-11-21

    Demand is increasing daily for large data storage systems that are useful for applications in spacecraft, space satellites, and space robots, which are all exposed to radiation-rich space environment. As candidates for use in space embedded systems, holographic storage systems are promising because they can easily provided the demanded large-storage capability. Particularly, holographic storage systems, which have no rotation mechanism, are demanded because they are virtually maintenance-free. Although a holographic memory itself is an extremely robust device even in a space radiation environment, its associated lasers and drive circuit devices are vulnerable. Such vulnerabilities sometimes engendered severe problems that prevent reading of all contents of the holographic memory, which is a turn-off failure mode of a laser array. This paper therefore presents a proposal for a recovery method for the turn-off failure mode of a laser array on a holographic storage system, and describes results of an experimental demonstration. © 2011 Optical Society of America

  1. Research and application on imaging technology of line structure light based on confocal microscopy

    Science.gov (United States)

    Han, Wenfeng; Xiao, Zexin; Wang, Xiaofen

    2009-11-01

    In 2005, the theory of line structure light confocal microscopy was put forward firstly in China by Xingyu Gao and Zexin Xiao in the Institute of Opt-mechatronics of Guilin University of Electronic Technology. Though the lateral resolution of line confocal microscopy can only reach or approach the level of the traditional dot confocal microscopy. But compared with traditional dot confocal microscopy, it has two advantages: first, by substituting line scanning for dot scanning, plane imaging only performs one-dimensional scanning, with imaging velocity greatly improved and scanning mechanism simplified, second, transfer quantity of light is greatly improved by substituting detection hairline for detection pinhole, and low illumination CCD is used directly to collect images instead of photoelectric intensifier. In order to apply the line confocal microscopy to practical system, based on the further research on the theory of the line confocal microscopy, imaging technology of line structure light is put forward on condition of implementation of confocal microscopy. Its validity and reliability are also verified by experiments.

  2. Near-field scanning optical microscopy based nanostructuring of glass

    International Nuclear Information System (INIS)

    Chimmalgi, A; Hwang, D J; Grigoropoulos, C P

    2007-01-01

    Nanofabrication, at lateral resolutions beyond the capability of conventional optical lithography techniques, is demonstrated here. Femtosecond laser was used in conjunction with Near-field Scanning Optical Microscopes (NSOMs) to nanostructure thin metal films. Also, the possibility of using these nanostructured metal films as masks to effectively transfer the pattern to the underlying substrate by wet etching process is shown. Two different optical nearfiled processing schemes were studied for near-field nanostructuring. In the first scheme, local field enhancement in the near-field of a scanning probe microscope (SPM) probe tip irradiated with femtosecond laser pulses was utilized (apertureless NSOM mode) and as a second approach, femtosecond laser beam was spatially confined by cantilevered NSOM fiber tip (apertured NOSM mode). The minimized heat- and shock-affected areas introduced during ultrafast laser based machining process, allows processing of even high conductivity thin metal films with minimized formation of any interfacial compounds between the metal films and the underlying substrate. Potential applications of this method may be in the fields of nanolithography, nanofluidics, nanoscale chemical and gas sensors, high-density data storage, nano-opto-electronics, as well as biotechnology related applications

  3. An automatic holographic adaptive phoropter

    Science.gov (United States)

    Amirsolaimani, Babak; Peyghambarian, N.; Schwiegerling, Jim; Bablumyan, Arkady; Savidis, Nickolaos; Peyman, Gholam

    2017-08-01

    Phoropters are the most common instrument used to detect refractive errors. During a refractive exam, lenses are flipped in front of the patient who looks at the eye chart and tries to read the symbols. The procedure is fully dependent on the cooperation of the patient to read the eye chart, provides only a subjective measurement of visual acuity, and can at best provide a rough estimate of the patient's vision. Phoropters are difficult to use for mass screenings requiring a skilled examiner, and it is hard to screen young children and the elderly etc. We have developed a simplified, lightweight automatic phoropter that can measure the optical error of the eye objectively without requiring the patient's input. The automatic holographic adaptive phoropter is based on a Shack-Hartmann wave front sensor and three computercontrolled fluidic lenses. The fluidic lens system is designed to be able to provide power and astigmatic corrections over a large range of corrections without the need for verbal feedback from the patient in less than 20 seconds.

  4. Holographic interferometry of high pressure

    International Nuclear Information System (INIS)

    McIlwain, M.E.

    1987-01-01

    Measurements in turbulent flows have been historically performed using various types of probes and optical diagnostic methods. In general, probes suffer from plasma perturbation effects and are single point determination methods. Optical methods appear to be better suited to determinations in turbulent flows, however interpretation of the resulting data can often be complex. Methods such as laser Doppler anemometry, which relies on entrained particles, suffers from the fact that particles small enough to be swept along by the plasma are usually melted or sublimed in the plasma. Light refraction or diffraction methods such as shadow photography, interferometry, and holography have also been used to observe plasma flows. These methods typically suffer from the difficulty of interpreting line of sight images and obtaining quantitative data. A new method based on multi-pass holographic interferometry will be discussed. This method has certain advantages which can significantly simplify the complexity of line of sight interferometry image deconvolution. When the method employs high speed cinematography, time resolved images of the plasma flow can be obtained. This method has been applied to both transferred and non-transferred arcs and various types of DC-plasma torch produced jets. These studies and conclusions as to the usefulness of the technique are presented

  5. A direct-view customer-oriented digital holographic camera

    Science.gov (United States)

    Besaga, Vira R.; Gerhardt, Nils C.; Maksimyak, Peter P.; Hofmann, Martin R.

    2018-01-01

    In this paper, we propose a direct-view digital holographic camera system consisting mostly of customer-oriented components. The camera system is based on standard photographic units such as camera sensor and objective and is adapted to operate under off-axis external white-light illumination. The common-path geometry of the holographic module of the system ensures direct-view operation. The system can operate in both self-reference and self-interference modes. As a proof of system operability, we present reconstructed amplitude and phase information of a test sample.

  6. Page Oriented Holographic Memories And Optical Pattern Recognition

    Science.gov (United States)

    Caulfield, H. J.

    1987-08-01

    In the twenty-two years since VanderLugt's introduction of holographic matched filtering, the intensive research carried out throughout the world has led to no applications in complex environment. This leads one to the suspicion that the VanderLugt filter technique is insufficiently complex to handle truly complex problems. Therefore, it is of great interest to increase the complexity of the VanderLugt filtering operation. We introduce here an approach to the real time filter assembly: use of page oriented holographic memories and optically addressed SLMs to achieve intelligent and fast reprogramming of the filters using a 10 4 to 10 6 stored pattern base.

  7. Nonvolatile Rad-Hard Holographic Memory

    Science.gov (United States)

    Chao, Tien-Hsin; Zhou, Han-Ying; Reyes, George; Dragoi, Danut; Hanna, Jay

    2001-01-01

    We are investigating a nonvolatile radiation-hardened (rad-hard) holographic memory technology. Recently, a compact holographic data storage (CHDS) breadboard utilizing an innovative electro-optic scanner has been built and demonstrated for high-speed holographic data storage and retrieval. The successful integration of this holographic memory breadboard has paved the way for follow-on radiation resistance test of the photorefractive (PR) crystal, Fe:LiNbO3. We have also started the investigation of using two-photon PR crystals that are doubly doped with atoms of iron group (Ti, Cr, Mn, Cu) and of rare-earth group (Nd, Tb) for nonvolatile holographic recordings.

  8. Gauge invariance and holographic renormalization

    Directory of Open Access Journals (Sweden)

    Keun-Young Kim

    2015-10-01

    Full Text Available We study the gauge invariance of physical observables in holographic theories under the local diffeomorphism. We find that gauge invariance is intimately related to the holographic renormalization: the local counter terms defined in the boundary cancel most of gauge dependences of the on-shell action as well as the divergences. There is a mismatch in the degrees of freedom between the bulk theory and the boundary one. We resolve this problem by noticing that there is a residual gauge symmetry (RGS. By extending the RGS such that it satisfies infalling boundary condition at the horizon, we can understand the problem in the context of general holographic embedding of a global symmetry at the boundary into the local gauge symmetry in the bulk.

  9. A new signal restoration method based on deconvolution of the Point Spread Function (PSF) for the Flat-Field Holographic Concave Grating UV spectrometer system

    Science.gov (United States)

    Dai, Honglin; Luo, Yongdao

    2013-12-01

    In recent years, with the development of the Flat-Field Holographic Concave Grating, they are adopted by all kinds of UV spectrometers. By means of single optical surface, the Flat-Field Holographic Concave Grating can implement dispersion and imaging that make the UV spectrometer system design quite compact. However, the calibration of the Flat-Field Holographic Concave Grating is very difficult. Various factors make its imaging quality difficult to be guaranteed. So we have to process the spectrum signal with signal restoration before using it. Guiding by the theory of signals and systems, and after a series of experiments, we found that our UV spectrometer system is a Linear Space- Variant System. It means that we have to measure PSF of every pixel of the system which contains thousands of pixels. Obviously, that's a large amount of calculation .For dealing with this problem, we proposes a novel signal restoration method. This method divides the system into several Linear Space-Invariant subsystems and then makes signal restoration with PSFs. Our experiments turn out that this method is effective and inexpensive.

  10. Simple concept for a wide-field lensless digital holographic microscope using a laser diode

    Directory of Open Access Journals (Sweden)

    Adinda-Ougba A.

    2015-09-01

    Full Text Available Wide-field, lensless digital holographic microscopy is a new microscopic imaging technique for telemedicine and for resource limited setting [1]. In this contribution we propose a very simple wide-field lensless digital holographic microscope using a laser diode. It is based on in-line digital holography which is capable to provide amplitude and phase images of a sample resulting from numerical reconstruction. The numerical reconstruction consists of the angular spectrum propagation method together with a phase retrieval algorithm. Amplitude and phase images of the sample with a resolution of ∽2 µm and with ∽24 mm2 field of view are obtained. We evaluate our setup by imaging first the 1951 USAF resolution test chart to verify the resolution. Second, we record holograms of blood smear and diatoms. The individual specimen can be easily identified after the numerical reconstruction. Our system is a very simple, compact and low-cost possibility of realizing a microscope capable of imaging biological samples. The availability of the phase provide topographic information of the sample extending the application of this system to be not only for biological sample but also for transparent microstructure. It is suitable for fault detection, shape and roughness measurements of these structures.

  11. Automated red blood cells extraction from holographic images using fully convolutional neural networks

    Science.gov (United States)

    Yi, Faliu; Moon, Inkyu; Javidi, Bahram

    2017-01-01

    In this paper, we present two models for automatically extracting red blood cells (RBCs) from RBCs holographic images based on a deep learning fully convolutional neural network (FCN) algorithm. The first model, called FCN-1, only uses the FCN algorithm to carry out RBCs prediction, whereas the second model, called FCN-2, combines the FCN approach with the marker-controlled watershed transform segmentation scheme to achieve RBCs extraction. Both models achieve good segmentation accuracy. In addition, the second model has much better performance in terms of cell separation than traditional segmentation methods. In the proposed methods, the RBCs phase images are first numerically reconstructed from RBCs holograms recorded with off-axis digital holographic microscopy. Then, some RBCs phase images are manually segmented and used as training data to fine-tune the FCN. Finally, each pixel in new input RBCs phase images is predicted into either foreground or background using the trained FCN models. The RBCs prediction result from the first model is the final segmentation result, whereas the result from the second model is used as the internal markers of the marker-controlled transform algorithm for further segmentation. Experimental results show that the given schemes can automatically extract RBCs from RBCs phase images and much better RBCs separation results are obtained when the FCN technique is combined with the marker-controlled watershed segmentation algorithm. PMID:29082078

  12. Holographic inspection of nuclear plant

    International Nuclear Information System (INIS)

    Gordon, A.L.; Armour, I.A.; Glanville, R.; Malcolm, G.J.; Wright, D.G.

    1988-01-01

    The high resolution, enormous depth of field and high tolerance to radiation of holography mean that it has great potential as an inspection tool in the nuclear industry. In addition, the ability of double-pulse holography to yield detailed information on vibration over the whole field of both large and small structures provides measurements that often cannot be obtained in any other way. This paper reviews the development of equipment for the holographic inspection of nuclear fuel elements; a portable holocamera for use inside reactors; and the application of holographic techniques for vibration measurements in a nuclear power station. (author)

  13. Holo-GPC: Holographic Generalized Phase Contrast

    DEFF Research Database (Denmark)

    Bañas, Andrew; Glückstad, Jesper

    2017-01-01

    Light shaping methods based on spatial phase-only modulation can be classified depending on whether they distribute multiple beams or shape the individual beams. Diffractive optics or holography can be classified as the former, as it spatially distributes a plurality of focal spots over a working...... of GPC in forming well-defined speckle-free shapes that can be distributed over an extended 3D volume through holographic means. The combined strengths of the two photon-efficient phase-only light shaping modalities open new possibilities for contemporary laser sculpting applications....

  14. Holographic description of large N gauge theory

    International Nuclear Information System (INIS)

    Lee, Sung-Sik

    2011-01-01

    Based on the earlier work [S.-S. Lee, Nucl. Rev. B 832 (2010) 567], we derive a holographic dual for the D-dimensional U(N) lattice gauge theory from a first principle construction. The resulting theory is a lattice field theory of closed loops, dubbed as lattice loop field theory which is defined on a (D+1)-dimensional space. The lattice loop field theory is well defined non-perturbatively, and it becomes weakly coupled and local in the large N limit with a large 't Hooft coupling.

  15. Field-based dynamic light scattering microscopy: theory and numerical analysis.

    Science.gov (United States)

    Joo, Chulmin; de Boer, Johannes F

    2013-11-01

    We present a theoretical framework for field-based dynamic light scattering microscopy based on a spectral-domain optical coherence phase microscopy (SD-OCPM) platform. SD-OCPM is an interferometric microscope capable of quantitative measurement of amplitude and phase of scattered light with high phase stability. Field-based dynamic light scattering (F-DLS) analysis allows for direct evaluation of complex-valued field autocorrelation function and measurement of localized diffusive and directional dynamic properties of biological and material samples with high spatial resolution. In order to gain insight into the information provided by F-DLS microscopy, theoretical and numerical analyses are performed to evaluate the effect of numerical aperture of the imaging optics. We demonstrate that sharp focusing of fields affects the measured diffusive and transport velocity, which leads to smaller values for the dynamic properties in the sample. An approach for accurately determining the dynamic properties of the samples is discussed.

  16. Scanning electron microscopy and transmission electron microscopy study of hot-deformed gamma-TiAl-based alloy microstructure.

    Science.gov (United States)

    Chrapoński, J; Rodak, K

    2006-09-01

    The aim of this work was to assess the changes in the microstructure of hot-deformed specimens made of alloys containing 46-50 at.% Al, 2 at.% Cr and 2 at.% Nb (and alloying additions such as carbon and boron) with the aid of scanning electron microscopy and transmission electron microscopy techniques. After homogenization and heat treatment performed in order to make diverse lamellae thickness, the specimens were compressed at 1000 degrees C. Transmission electron microscopy examinations of specimens after the compression test revealed the presence of heavily deformed areas with a high density of dislocation. Deformation twins were also observed. Dynamically recrystallized grains were revealed. For alloys no. 2 and no. 3, the recovery and recrystallization processes were more extensive than for alloy no. 1.

  17. Atomic force microscopy imaging to measure precipitate volume fraction in nickel-based superalloys

    International Nuclear Information System (INIS)

    Bourhettar, A.; Troyon, M.; Hazotte, A.

    1995-01-01

    In nickel-based superalloys, quantitative analysis of scanning electron microscopy images fails in providing accurate microstructural data, whereas more efficient techniques are very time-consuming. As an alternative approach, the authors propose to perform quantitative analysis of atomic force microscopy images of polished/etched surfaces (quantitative microprofilometry). This permits the measurement of microstructural parameters and the depth of etching, which is the main source of measurement bias. Thus, nonbiased estimations can be obtained by extrapolation of the measurements up to zero etching depth. In this article, the authors used this approach to estimate the volume fraction of γ' precipitates in a nickel-based superalloy single crystal. Atomic force microscopy images of samples etched for different times show definition, homogeneity, and contrast high enough to perform image analysis. The result after extrapolation is in very good agreement with volume fraction values available from published reports

  18. Characterization of the Micro-shell Surface Using Holographic Measurements

    Energy Technology Data Exchange (ETDEWEB)

    Sandras, F.; Hermerel, C.; Choux, A.; Merillot, P.; Pin, G.; Jeannot, L. [CEA Valduc, Dept Rech Mat Nucl, Serv Microcibles, 21 - Is-sur-Tille (France)

    2009-05-15

    To characterize the shape, the quality, and the roughness of micro-shells, typically used technologies are scanning electron microscopy, scanning interferometric microscopy, or atomic force microscopy. One of the drawbacks of these techniques is that they are generally slow because of their scanning process. Digital holographic microscopy technology is an innovation that can offer ability adapted to these studies. It captures holograms instead of intensity images, as done by conventional microscopes. The holograms are then digitally interpreted (10 per second) to reconstruct a double image, one for the intensity and another one for the phase. Using a rotation axis, the bump counting for the complete micro-shell surface is possible with a very high speed. Using an image stitching software, mapping can be done in a few minutes. Wavelets such as 'Mexican hat' are used to model the bumps. Each bump can then be characterized on the map by its position, diameter, and height. (authors)

  19. High-speed holographic camera

    International Nuclear Information System (INIS)

    Novaro, Marc

    The high-speed holographic camera is a disgnostic instrument using holography as an information storing support. It allows us to take 10 holograms, of an object, with exposures times of 1,5ns, separated in time by 1 or 2ns. In order to get these results easily, no mobile part is used in the set-up [fr

  20. Conformal symmetry and holographic cosmology

    NARCIS (Netherlands)

    Bzowski, A.W.

    2013-01-01

    This thesis presents a novel approach to cosmology using gauge/gravity duality. Analysis of the implications of conformal invariance in field theories leads to quantitative cosmological predictions which are in agreement with current data. Furthermore, holographic cosmology extends the theory of

  1. Holographic complexity and spacetime singularities

    Energy Technology Data Exchange (ETDEWEB)

    Barbón, José L.F. [Instituto de Física Teórica IFT UAM/CSIC,C/ Nicolás Cabrera 13, Campus Universidad Autónoma de Madrid,Madrid 28049 (Spain); Rabinovici, Eliezer [Racah Institute of Physics, The Hebrew University,Jerusalem 91904 (Israel); Laboratoire de Physique Théorique et Hautes Energies, Université Pierre et Marie Curie, 4 Place Jussieu, 75252 Paris Cedex 05 (France)

    2016-01-15

    We study the evolution of holographic complexity in various AdS/CFT models containing cosmological crunch singularities. We find that a notion of complexity measured by extremal bulk volumes tends to decrease as the singularity is approached in CFT time, suggesting that the corresponding quantum states have simpler entanglement structure at the singularity.

  2. Holographic complexity and spacetime singularities

    International Nuclear Information System (INIS)

    Barbón, José L.F.; Rabinovici, Eliezer

    2016-01-01

    We study the evolution of holographic complexity in various AdS/CFT models containing cosmological crunch singularities. We find that a notion of complexity measured by extremal bulk volumes tends to decrease as the singularity is approached in CFT time, suggesting that the corresponding quantum states have simpler entanglement structure at the singularity.

  3. Computer-generated holographic near-eye display system based on LCoS phase only modulator

    Science.gov (United States)

    Sun, Peng; Chang, Shengqian; Zhang, Siman; Xie, Ting; Li, Huaye; Liu, Siqi; Wang, Chang; Tao, Xiao; Zheng, Zhenrong

    2017-09-01

    Augmented reality (AR) technology has been applied in various areas, such as large-scale manufacturing, national defense, healthcare, movie and mass media and so on. An important way to realize AR display is using computer-generated hologram (CGH), which is accompanied by low image quality and heavy computing defects. Meanwhile, the diffraction of Liquid Crystal on Silicon (LCoS) has a negative effect on image quality. In this paper, a modified algorithm based on traditional Gerchberg-Saxton (GS) algorithm was proposed to improve the image quality, and new method to establish experimental system was used to broaden field of view (FOV). In the experiment, undesired zero-order diffracted light was eliminated and high definition 2D image was acquired with FOV broadened to 36.1 degree. We have also done some pilot research in 3D reconstruction with tomography algorithm based on Fresnel diffraction. With the same experimental system, experimental results demonstrate the feasibility of 3D reconstruction. These modifications are effective and efficient, and may provide a better solution in AR realization.

  4. Implementation of all-optical reversible logic gate based on holographic laser induced grating using azo-dye doped polymers

    Science.gov (United States)

    Forsati, Rana; Valipour Ebrahimi, Sara; Navi, Keivan; Mohajerani, Ezeddin; Jashnsaz, Hossein

    2013-02-01

    Increasing demand for power reduction in computer systems has led to new trends in computations and computer design including reversible computing. Its main aim is to eliminate power dissipation in logical elements but can have some other advantages such as data security and error prevention. Because of interesting properties of reversible computing, implementing computing devices with reversible manner is the only way to make the reversible computing a reality. In recent years, reversible logic has turned out to be a promising computing paradigm having application in CMOS, nanotechnology, quantum computing and optical computing. In this paper, we propose and realize a novel implementation of Toffoli gate in all-optical domain. We have explained its principle of operations and described an actual experimental implementation. The all-optical reversible gate presented in this paper will be useful in different applications such as arithmetic and logical operations in the domain of reversible logic-based computing.

  5. A Holographic Microscope for Detection of Microorganisms on Icy Worlds

    Science.gov (United States)

    Lindensmith, C. A.; Nadeau, J. L.; Deming, J. W.; Showalter, G. M.; Rider, S.; Bedrossian, M.

    2015-12-01

    Holography is a well-established imaging technique that uses the interference of light to record and reproduce three-dimensional images of objects. Its use began in the 1960s with the invention of the laser. Digital holographic microscopy (DHM) has several advantages over ordinary imaging microscopy which make it ideal for field and astrobiology use, including no need for focus or scanning so that instruments are readily made autonomous. DHM can produce simultaneous bright-field and quantitative phase-contrast images of the same field, providing additional information about transparent objects, e.g., refractive index and/or thickness; thus it inherently supports effective label-free imaging. We have built a fieldable DHM for detection of microorganisms in bodies of water and in brines collected from sea ice. Ice that appears solid to the eye contains interconnected brine-filled microscopic pores and veins which are occupied by populations of prokaryotes and eukaryotes. The presence of life in "solid" ice has important implications for exploration of icy worlds, where it is unlikely that the first missions will be able to access the subsurface oceans. Using this new instrument, we examined several dozen samples from three different sites around Nuuk, Greenland. In all samples, mixed populations of both prokaryotic and eukaryotic microorganisms were observed. Many of the organisms were motile immediately upon extraction from sea ice, and others became motile after warming or addition of sugars and/or amino acids. Meaningful motility was readily distinguished from turbulence or fluid flow. The spatial resolution of the instrument was better than 1 μm, leading to unambiguous recognition of subcellular structures in eukaryotes, including nuclei and chloroplasts. We present mission scenrios for both orbiters and landers in which DHM may be used as a valuable complement to chemical-based life detection techniques for discovery of cellular life on icy worlds.

  6. The Electron Microscopy Outreach Program: A Web-based resource for research and education.

    Science.gov (United States)

    Sosinsky, G E; Baker, T S; Hand, G; Ellisman, M H

    1999-01-01

    We have developed a centralized World Wide Web (WWW)-based environment that serves as a resource of software tools and expertise for biological electron microscopy. A major focus is molecular electron microscopy, but the site also includes information and links on structural biology at all levels of resolution. This site serves to help integrate or link structural biology techniques in accordance with user needs. The WWW site, called the Electron Microscopy (EM) Outreach Program (URL: http://emoutreach.sdsc.edu), provides scientists with computational and educational tools for their research and edification. In particular, we have set up a centralized resource containing course notes, references, and links to image analysis and three-dimensional reconstruction software for investigators wanting to learn about EM techniques either within or outside of their fields of expertise. Copyright 1999 Academic Press.

  7. Numerical tilting compensation in microscopy based on wavefront sensing using transport of intensity equation method

    Science.gov (United States)

    Hu, Junbao; Meng, Xin; Wei, Qi; Kong, Yan; Jiang, Zhilong; Xue, Liang; Liu, Fei; Liu, Cheng; Wang, Shouyu

    2018-03-01

    Wide-field microscopy is commonly used for sample observations in biological research and medical diagnosis. However, the tilting error induced by the oblique location of the image recorder or the sample, as well as the inclination of the optical path often deteriorates the imaging quality. In order to eliminate the tilting in microscopy, a numerical tilting compensation technique based on wavefront sensing using transport of intensity equation method is proposed in this paper. Both the provided numerical simulations and practical experiments prove that the proposed technique not only accurately determines the tilting angle with simple setup and procedures, but also compensates the tilting error for imaging quality improvement even in the large tilting cases. Considering its simple systems and operations, as well as image quality improvement capability, it is believed the proposed method can be applied for tilting compensation in the optical microscopy.

  8. Emulation and design of terahertz reflection-mode confocal scanning microscopy based on virtual pinhole

    Science.gov (United States)

    Yang, Yong-fa; Li, Qi

    2014-12-01

    In the practical application of terahertz reflection-mode confocal scanning microscopy, the size of detector pinhole is an important factor that determines the performance of spatial resolution characteristic of the microscopic system. However, the use of physical pinhole brings some inconvenience to the experiment and the adjustment error has a great influence on the experiment result. Through reasonably selecting the parameter of matrix detector virtual pinhole (VPH), it can efficiently approximate the physical pinhole. By using this approach, the difficulty of experimental calibration is reduced significantly. In this article, an imaging scheme of terahertz reflection-mode confocal scanning microscopy that is based on the matrix detector VPH is put forward. The influence of detector pinhole size on the axial resolution of confocal scanning microscopy is emulated and analyzed. Then, the parameter of VPH is emulated when the best axial imaging performance is reached.

  9. Holographic complexity in gauge/string superconductors

    Directory of Open Access Journals (Sweden)

    Davood Momeni

    2016-05-01

    Full Text Available Following a methodology similar to [1], we derive a holographic complexity for two dimensional holographic superconductors (gauge/string superconductors with backreactions. Applying a perturbation method proposed by Kanno in Ref. [2], we study behaviors of the complexity for a dual quantum system near critical points. We show that when a system moves from the normal phase (T>Tc to the superconductor phase (Tholographic complexity will be divergent.

  10. Homodyne detection of holographic memory systems

    Science.gov (United States)

    Urness, Adam C.; Wilson, William L.; Ayres, Mark R.

    2014-09-01

    We present a homodyne detection system implemented for a page-wise holographic memory architecture. Homodyne detection by holographic memory systems enables phase quadrature multiplexing (doubling address space), and lower exposure times (increasing read transfer rates). It also enables phase modulation, which improves signal-to-noise ratio (SNR) to further increase data capacity. We believe this is the first experimental demonstration of homodyne detection for a page-wise holographic memory system suitable for a commercial design.

  11. Holographic kinetic k-essence model

    Energy Technology Data Exchange (ETDEWEB)

    Cruz, Norman [Departamento de Fisica, Facultad de Ciencia, Universidad de Santiago de Chile, Casilla 307, Santiago (Chile)], E-mail: ncruz@lauca.usach.cl; Gonzalez-Diaz, Pedro F.; Rozas-Fernandez, Alberto [Colina de los Chopos, Instituto de Fisica Fundamental, Consejo Superior de Investigaciones Cientificas, Serrano 121, 28006 Madrid (Spain)], E-mail: a.rozas@cfmac.csic.es; Sanchez, Guillermo [Departamento de Matematica y Ciencia de la Computacion, Facultad de Ciencia, Universidad de Santiago de Chile, Casilla 307, Santiago (Chile)], E-mail: gsanchez@usach.cl

    2009-08-31

    We consider a connection between the holographic dark energy density and the kinetic k-essence energy density in a flat FRW universe. With the choice c{>=}1, the holographic dark energy can be described by a kinetic k-essence scalar field in a certain way. In this Letter we show this kinetic k-essential description of the holographic dark energy with c{>=}1 and reconstruct the kinetic k-essence function F(X)

  12. Prospects of linear reconstruction in atomic resolution electron holographic tomography

    International Nuclear Information System (INIS)

    Krehl, Jonas; Lubk, Axel

    2015-01-01

    Tomography commonly requires a linear relation between the measured signal and the underlying specimen property; for Electron Holographic Tomography this is given by the Phase Grating Approximation (PGA). While largely valid at medium resolution, discrepancies arise at high resolution imaging conditions. We set out to investigate the artefacts that are produced if the reconstruction still assumes the PGA even with an atomic resolution tilt series. To forego experimental difficulties the holographic tilt series was simulated. The reconstructed electric potential clearly shows peaks at the positions of the atoms. These peaks have characterisitic deformations, which can be traced back to the defocus a particular atom has in the holograms of the tilt series. Exchanging an atom for one of a different atomic number results in a significant change in the reconstructed potential that is well contained within the atom's peak. - Highlights: • We simulate a holographic tilt series of a nanocrystal with atomic resolution. • Using PGA-based Holographic Tomography we reconstruct the atomic structure. • The reconstruction shows characteristic artefacts, chiefly caused by defocus. • Changing one atom's Z produces a well localised in the reconstruction

  13. Prospects of linear reconstruction in atomic resolution electron holographic tomography

    Energy Technology Data Exchange (ETDEWEB)

    Krehl, Jonas, E-mail: Jonas.Krehl@triebenberg.de; Lubk, Axel

    2015-03-15

    Tomography commonly requires a linear relation between the measured signal and the underlying specimen property; for Electron Holographic Tomography this is given by the Phase Grating Approximation (PGA). While largely valid at medium resolution, discrepancies arise at high resolution imaging conditions. We set out to investigate the artefacts that are produced if the reconstruction still assumes the PGA even with an atomic resolution tilt series. To forego experimental difficulties the holographic tilt series was simulated. The reconstructed electric potential clearly shows peaks at the positions of the atoms. These peaks have characterisitic deformations, which can be traced back to the defocus a particular atom has in the holograms of the tilt series. Exchanging an atom for one of a different atomic number results in a significant change in the reconstructed potential that is well contained within the atom's peak. - Highlights: • We simulate a holographic tilt series of a nanocrystal with atomic resolution. • Using PGA-based Holographic Tomography we reconstruct the atomic structure. • The reconstruction shows characteristic artefacts, chiefly caused by defocus. • Changing one atom's Z produces a well localised in the reconstruction.

  14. Holographic duality: Stealing dimensions from metals

    Science.gov (United States)

    Zaanen, Jan

    2013-10-01

    Although electrically charged black holes seem remote from superconductors and strange metals in the laboratory, they might be intimately related by the holographic dualities discovered in string theory.

  15. Holographic Two-Photon Induced Photopolymerization

    Data.gov (United States)

    Federal Laboratory Consortium — Holographic two-photon-induced photopolymerization (HTPIP) offers distinct advantages over conventional one-photon-induced photopolymerization and current techniques...

  16. Electromagnetic holographic sensitivity field of two-phase flow in horizontal wells

    Science.gov (United States)

    Zhang, Kuo; Wu, Xi-Ling; Yan, Jing-Fu; Cai, Jia-Tie

    2017-03-01

    Electromagnetic holographic data are characterized by two modes, suggesting that image reconstruction requires a dual-mode sensitivity field as well. We analyze an electromagnetic holographic field based on tomography theory and Radon inverse transform to derive the expression of the electromagnetic holographic sensitivity field (EMHSF). Then, we apply the EMHSF calculated by using finite-element methods to flow simulations and holographic imaging. The results suggest that the EMHSF based on the partial derivative of radius of the complex electric potential φ is closely linked to the Radon inverse transform and encompasses the sensitivities of the amplitude and phase data. The flow images obtained with inversion using EMHSF better agree with the actual flow patterns. The EMHSF overcomes the limitations of traditional single-mode sensitivity fields.

  17. Holographic Renormalization in Dense Medium

    International Nuclear Information System (INIS)

    Park, Chanyong

    2014-01-01

    The holographic renormalization of a charged black brane with or without a dilaton field, whose dual field theory describes a dense medium at finite temperature, is investigated in this paper. In a dense medium, two different thermodynamic descriptions are possible due to an additional conserved charge. These two different thermodynamic ensembles are classified by the asymptotic boundary condition of the bulk gauge field. It is also shown that in the holographic renormalization regularity of all bulk fields can reproduce consistent thermodynamic quantities and that the Bekenstein-Hawking entropy is nothing but the renormalized thermal entropy of the dual field theory. Furthermore, we find that the Reissner-Nordström AdS black brane is dual to a theory with conformal matter as expected, whereas a charged black brane with a nontrivial dilaton profile is mapped to a theory with nonconformal matter although its leading asymptotic geometry still remains as AdS space

  18. Holographic models with anisotropic scaling

    Science.gov (United States)

    Brynjolfsson, E. J.; Danielsson, U. H.; Thorlacius, L.; Zingg, T.

    2013-12-01

    We consider gravity duals to d+1 dimensional quantum critical points with anisotropic scaling. The primary motivation comes from strongly correlated electron systems in condensed matter theory but the main focus of the present paper is on the gravity models in their own right. Physics at finite temperature and fixed charge density is described in terms of charged black branes. Some exact solutions are known and can be used to obtain a maximally extended spacetime geometry, which has a null curvature singularity inside a single non-degenerate horizon, but generic black brane solutions in the model can only be obtained numerically. Charged matter gives rise to black branes with hair that are dual to the superconducting phase of a holographic superconductor. Our numerical results indicate that holographic superconductors with anisotropic scaling have vanishing zero temperature entropy when the back reaction of the hair on the brane geometry is taken into account.

  19. Holographic Chern-Simons defects

    International Nuclear Information System (INIS)

    Fujita, Mitsutoshi; Melby-Thompson, Charles M.; Meyer, René; Sugimoto, Shigeki

    2016-01-01

    We study SU(N) Yang-Mills-Chern-Simons theory in the presence of defects that shift the Chern-Simons level from a holographic point of view by embedding the system in string theory. The model is a D3-D7 system in Type IIB string theory, whose gravity dual is given by the AdS soliton background with probe D7 branes attaching to the AdS boundary along the defects. We holographically renormalize the free energy of the defect system with sources, from which we obtain the correlation functions for certain operators naturally associated to these defects. We find interesting phase transitions when the separation of the defects as well as the temperature are varied. We also discuss some implications for the Fractional Quantum Hall Effect and for 2-dimensional QCD.

  20. Baryon physics in holographic QCD

    Directory of Open Access Journals (Sweden)

    Alex Pomarol

    2009-03-01

    Full Text Available In a simple holographic model for QCD in which the Chern–Simons term is incorporated to take into account the QCD chiral anomaly, we show that baryons arise as stable solitons which are the 5D analogs of 4D skyrmions. Contrary to 4D skyrmions and previously considered holographic scenarios, these solitons have sizes larger than the inverse cut-off of the model, and therefore they are predictable within our effective field theory approach. We perform a numerical determination of several static properties of the nucleons and find a satisfactory agreement with data. We also calculate the amplitudes of “anomalous” processes induced by the Chern–Simons term in the meson sector, such as ω→πγ and ω→3π. A combined fit to baryonic and mesonic observables leads to an agreement with experiments within 16%.

  1. Overview of fast algorithm in 3D dynamic holographic display

    Science.gov (United States)

    Liu, Juan; Jia, Jia; Pan, Yijie; Wang, Yongtian

    2013-08-01

    3D dynamic holographic display is one of the most attractive techniques for achieving real 3D vision with full depth cue without any extra devices. However, huge 3D information and data should be preceded and be computed in real time for generating the hologram in 3D dynamic holographic display, and it is a challenge even for the most advanced computer. Many fast algorithms are proposed for speeding the calculation and reducing the memory usage, such as:look-up table (LUT), compressed look-up table (C-LUT), split look-up table (S-LUT), and novel look-up table (N-LUT) based on the point-based method, and full analytical polygon-based methods, one-step polygon-based method based on the polygon-based method. In this presentation, we overview various fast algorithms based on the point-based method and the polygon-based method, and focus on the fast algorithm with low memory usage, the C-LUT, and one-step polygon-based method by the 2D Fourier analysis of the 3D affine transformation. The numerical simulations and the optical experiments are presented, and several other algorithms are compared. The results show that the C-LUT algorithm and the one-step polygon-based method are efficient methods for saving calculation time. It is believed that those methods could be used in the real-time 3D holographic display in future.

  2. Reducing microscopy-based malaria misdiagnosis in a low-resource area of Tanzania.

    Science.gov (United States)

    Allen, Lisa K; Hatfield, Jennifer M; Manyama, Mange

    2013-01-01

    Misdiagnosis of malaria is a major problem in Africa leading not only to incorrect individual level treatment, but potentially the acceleration of the spread of drug resistance in low-transmission areas. In this paper we report on the outcomes of a simple intervention that utilized a social entrepreneurship approach (SEA) to reduce misdiagnosis associated with hospital-based microscopy of malaria in a low-transmission area of rural Tanzania. A pre-post assessment was conducted on patients presenting to the hospital outpatient department with malaria and non-malaria like symptoms in January 2009 (pre-intervention) and June 2009 (post-intervention). All participants were asked a health seeking behavior questionnaire and blood samples were taken for local and quality control microscopy. Multivariate logistic regression was conducted to determine magnitude of misdiagnosis with local microscopy pre- versus- post intervention. Local microscopy pre-intervention specificity was 29.5% (95% CI = 21.6% - 38.4%) whereas the post intervention specificity was 68.6% (95% CI = 60.2% - 76.2%). Both pre and post intervention sensitivity were difficult to determine due to an unexpected low number of true positive cases. The proportion of participants misdiagnosed pre-intervention was 70.2% (95%CI = 61.3%-78.0%) as compared to 30.6% (95%CI = 23.2%-38.8%) post-intervention. This resulted in a 39.6% reduction in misdiagnosis of malaria at the local hospital. The magnitude of misdiagnosis for the pre-intervention participants was 5.3 (95%CI = 3.1-9.3) that of the post-intervention participants. In conclusion, this study provides evidence that a simple intervention can meaningfully reduce the magnitude of microscopy-based misdiagnosis of malaria for those individuals seeking treatment for uncomplicated malaria. We anticipate that this intervention will facilitate a valuable and sustainable change in malaria diagnosis at the local hospital.

  3. Constructive use of holographic projections

    International Nuclear Information System (INIS)

    Schroer, Bert

    2008-01-01

    Revisiting the old problem of existence of interacting models of QFT with new conceptual ideas and mathematical tools, one arrives at a novel view about the nature of QFT. The recent success of algebraic methods in establishing the existence of factorizing models suggests new directions for a more intrinsic constructive approach beyond Lagrangian quantization. Holographic projection simplifies certain properties of the bulk theory and hence is a promising new tool for these new attempts. (author)

  4. Constructive use of holographic projections

    Energy Technology Data Exchange (ETDEWEB)

    Schroer, Bert [Centro Brasileiro de Pesquisas Fisicas (CBPF), Rio de Janeiro, RJ (Brazil); Institut fuer Theoretische Physik der FU, Berlin (Germany)

    2008-07-01

    Revisiting the old problem of existence of interacting models of QFT with new conceptual ideas and mathematical tools, one arrives at a novel view about the nature of QFT. The recent success of algebraic methods in establishing the existence of factorizing models suggests new directions for a more intrinsic constructive approach beyond Lagrangian quantization. Holographic projection simplifies certain properties of the bulk theory and hence is a promising new tool for these new attempts. (author)

  5. Holographic multiverse and conformal invariance

    Energy Technology Data Exchange (ETDEWEB)

    Garriga, Jaume [Departament de Física Fonamental i Institut de Ciències del Cosmos, Universitat de Barcelona, Martí i Franquès 1, 08193 Barcelona (Spain); Vilenkin, Alexander, E-mail: jaume.garriga@ub.edu, E-mail: vilenkin@cosmos.phy.tufts.edu [Institute of Cosmology, Department of Physics and Astronomy, Tufts University, 212 College Ave., Medford, MA 02155 (United States)

    2009-11-01

    We consider a holographic description of the inflationary multiverse, according to which the wave function of the universe is interpreted as the generating functional for a lower dimensional Euclidean theory. We analyze a simple model where transitions between inflationary vacua occur through bubble nucleation, and the inflating part of spacetime consists of de Sitter regions separated by thin bubble walls. In this model, we present some evidence that the dual theory is conformally invariant in the UV.

  6. Holographic multiverse and conformal invariance

    International Nuclear Information System (INIS)

    Garriga, Jaume; Vilenkin, Alexander

    2009-01-01

    We consider a holographic description of the inflationary multiverse, according to which the wave function of the universe is interpreted as the generating functional for a lower dimensional Euclidean theory. We analyze a simple model where transitions between inflationary vacua occur through bubble nucleation, and the inflating part of spacetime consists of de Sitter regions separated by thin bubble walls. In this model, we present some evidence that the dual theory is conformally invariant in the UV

  7. DMD-based LED-illumination super-resolution and optical sectioning microscopy.

    Science.gov (United States)

    Dan, Dan; Lei, Ming; Yao, Baoli; Wang, Wen; Winterhalder, Martin; Zumbusch, Andreas; Qi, Yujiao; Xia, Liang; Yan, Shaohui; Yang, Yanlong; Gao, Peng; Ye, Tong; Zhao, Wei

    2013-01-01

    Super-resolution three-dimensional (3D) optical microscopy has incomparable advantages over other high-resolution microscopic technologies, such as electron microscopy and atomic force microscopy, in the study of biological molecules, pathways and events in live cells and tissues. We present a novel approach of structured illumination microscopy (SIM) by using a digital micromirror device (DMD) for fringe projection and a low-coherence LED light for illumination. The lateral resolution of 90 nm and the optical sectioning depth of 120 μm were achieved. The maximum acquisition speed for 3D imaging in the optical sectioning mode was 1.6×10(7) pixels/second, which was mainly limited by the sensitivity and speed of the CCD camera. In contrast to other SIM techniques, the DMD-based LED-illumination SIM is cost-effective, ease of multi-wavelength switchable and speckle-noise-free. The 2D super-resolution and 3D optical sectioning modalities can be easily switched and applied to either fluorescent or non-fluorescent specimens.

  8. Nanoparticle discrimination based on wavelength and lifetime-multiplexed cathodoluminescence microscopy.

    Science.gov (United States)

    Garming, Mathijs W H; Weppelman, I Gerward C; de Boer, Pascal; Martínez, Felipe Perona; Schirhagl, Romana; Hoogenboom, Jacob P; Moerland, Robert J

    2017-08-31

    Nanomaterials can be identified in high-resolution electron microscopy images using spectrally-selective cathodoluminescence. Capabilities for multiplex detection can however be limited, e.g., due to spectral overlap or availability of filters. Also, the available photon flux may be limited due to degradation under electron irradiation. Here, we demonstrate single-pass cathodoluminescence-lifetime based discrimination of different nanoparticles, using a pulsed electron beam. We also show that cathodoluminescence lifetime is a robust parameter even when the nanoparticle cathodoluminescence intensity decays over an order of magnitude. We create lifetime maps, where the lifetime of the cathodoluminescence emission is correlated with the emission intensity and secondary-electron images. The consistency of lifetime-based discrimination is verified by also correlating the emission wavelength and the lifetime of nanoparticles. Our results show how cathodoluminescence lifetime provides an additional channel of information in electron microscopy.

  9. Carbazole Containing Copolymers: Synthesis, Characterization, and Applications in Reversible Holographic Recording

    Directory of Open Access Journals (Sweden)

    Bénédicte Mailhot-Jensen

    2010-01-01

    Full Text Available Carbazolic copolymers have been developed to be used in reversible holographic recording. This paper describes a complete analysis, from synthesis of the material to its applications, together with the corresponding characterizations. The investigated materials were photosensitive copolymers obtained from carbazolylalkylmethacrylates (CEM and octylmethacrylate (OMA. A detailed investigation was undertaken involving infrared spectroscopy and NMR techniques, 1H, 13C, COSY, and HSQC, in order to establish the chemical structure and the composition of the copolymers. Holographic recording characteristics were investigated with one- and two-layer photothermoplastic carriers. The two-layer carrier contains separate photosensitive and thermoplastic layers and gives the best holographic response. The surface of microstructured samples has been characterized by atomic force microscopy analysis. It is shown that via a photothermoplastic recording process, it is possible to record and read holograms practically in real time (~3 s with a diffraction efficiency of 10% and a spatial resolution higher than 1000 mm−1.

  10. Solid-state nuclear-spin quantum computer based on magnetic resonance force microscopy

    International Nuclear Information System (INIS)

    Berman, G. P.; Doolen, G. D.; Hammel, P. C.; Tsifrinovich, V. I.

    2000-01-01

    We propose a nuclear-spin quantum computer based on magnetic resonance force microscopy (MRFM). It is shown that an MRFM single-electron spin measurement provides three essential requirements for quantum computation in solids: (a) preparation of the ground state, (b) one- and two-qubit quantum logic gates, and (c) a measurement of the final state. The proposed quantum computer can operate at temperatures up to 1 K. (c) 2000 The American Physical Society

  11. Segmentation-based retrospective shading correction in fluorescence microscopy E. coli images for quantitative analysis

    Science.gov (United States)

    Mai, Fei; Chang, Chunqi; Liu, Wenqing; Xu, Weichao; Hung, Yeung S.

    2009-10-01

    Due to the inherent imperfections in the imaging process, fluorescence microscopy images often suffer from spurious intensity variations, which is usually referred to as intensity inhomogeneity, intensity non uniformity, shading or bias field. In this paper, a retrospective shading correction method for fluorescence microscopy Escherichia coli (E. Coli) images is proposed based on segmentation result. Segmentation and shading correction are coupled together, so we iteratively correct the shading effects based on segmentation result and refine the segmentation by segmenting the image after shading correction. A fluorescence microscopy E. Coli image can be segmented (based on its intensity value) into two classes: the background and the cells, where the intensity variation within each class is close to zero if there is no shading. Therefore, we make use of this characteristics to correct the shading in each iteration. Shading is mathematically modeled as a multiplicative component and an additive noise component. The additive component is removed by a denoising process, and the multiplicative component is estimated using a fast algorithm to minimize the intra-class intensity variation. We tested our method on synthetic images and real fluorescence E.coli images. It works well not only for visual inspection, but also for numerical evaluation. Our proposed method should be useful for further quantitative analysis especially for protein expression value comparison.

  12. Available number of multiplexed holograms based on signal-to-noise ratio analysis in reflection-type holographic memory using three-dimensional speckle-shift multiplexing.

    Science.gov (United States)

    Nishizaki, Tatsuya; Matoba, Osamu; Nitta, Kouichi

    2014-09-01

    The recording properties of three-dimensional speckle-shift multiplexing in reflection-type holographic memory are analyzed numerically. Three-dimensional recording can increase the number of multiplexed holograms by suppressing the cross-talk noise from adjacent holograms by using depth-direction multiplexing rather than in-plane multiplexing. Numerical results indicate that the number of multiplexed holograms in three-layer recording can be increased by 1.44 times as large as that of a single-layer recording when an acceptable signal-to-noise ratio is set to be 2 when NA=0.43 and the thickness of the recording medium is 0.5 mm.

  13. Toward a holographic theory for general spacetimes

    Science.gov (United States)

    Nomura, Yasunori; Salzetta, Nico; Sanches, Fabio; Weinberg, Sean J.

    2017-04-01

    We study a holographic theory of general spacetimes that does not rely on the existence of asymptotic regions. This theory is to be formulated in a holographic space. When a semiclassical description is applicable, the holographic space is assumed to be a holographic screen: a codimension-1 surface that is capable of encoding states of the gravitational spacetime. Our analysis is guided by conjectured relationships between gravitational spacetime and quantum entanglement in the holographic description. To understand basic features of this picture, we catalog predictions for the holographic entanglement structure of cosmological spacetimes. We find that qualitative features of holographic entanglement entropies for such spacetimes differ from those in AdS/CFT but that the former reduce to the latter in the appropriate limit. The Hilbert space of the theory is analyzed, and two plausible structures are found: a direct-sum and "spacetime-equals-entanglement" structure. The former preserves a naive relationship between linear operators and observable quantities, while the latter respects a more direct connection between holographic entanglement and spacetime. We also discuss the issue of selecting a state in quantum gravity, in particular how the state of the multiverse may be selected in the landscape.

  14. High-spatial-resolution sub-surface imaging using a laser-based acoustic microscopy technique.

    Science.gov (United States)

    Balogun, Oluwaseyi; Cole, Garrett D; Huber, Robert; Chinn, Diane; Murray, Todd W; Spicer, James B

    2011-01-01

    Scanning acoustic microscopy techniques operating at frequencies in the gigahertz range are suitable for the elastic characterization and interior imaging of solid media with micrometer-scale spatial resolution. Acoustic wave propagation at these frequencies is strongly limited by energy losses, particularly from attenuation in the coupling media used to transmit ultrasound to a specimen, leading to a decrease in the depth in a specimen that can be interrogated. In this work, a laser-based acoustic microscopy technique is presented that uses a pulsed laser source for the generation of broadband acoustic waves and an optical interferometer for detection. The use of a 900-ps microchip pulsed laser facilitates the generation of acoustic waves with frequencies extending up to 1 GHz which allows for the resolution of micrometer-scale features in a specimen. Furthermore, the combination of optical generation and detection approaches eliminates the use of an ultrasonic coupling medium, and allows for elastic characterization and interior imaging at penetration depths on the order of several hundred micrometers. Experimental results illustrating the use of the laser-based acoustic microscopy technique for imaging micrometer-scale subsurface geometrical features in a 70-μm-thick single-crystal silicon wafer with a (100) orientation are presented.

  15. Lensfree microscopy on a cellphone

    Science.gov (United States)

    Tseng, Derek; Mudanyali, Onur; Oztoprak, Cetin; Isikman, Serhan O.; Sencan, Ikbal; Yaglidere, Oguzhan; Ozcan, Aydogan

    2010-01-01

    We demonstrate lensfree digital microscopy on a cellphone. This compact and light-weight holographic microscope installed on a cellphone does not utilize any lenses, lasers or other bulky optical components and it may offer a cost-effective tool for telemedicine applications to address various global health challenges. Weighing ~38 grams (cellphone where the samples are loaded from the side, and are vertically illuminated by a simple light-emitting diode (LED). This incoherent LED light is then scattered from each micro-object to coherently interfere with the background light, creating the lensfree hologram of each object on the detector array of the cellphone. These holographic signatures captured by the cellphone permit reconstruction of microscopic images of the objects through rapid digital processing. We report the performance of this lensfree cellphone microscope by imaging various sized micro-particles, as well as red blood cells, white blood cells, platelets and a waterborne parasite (Giardia lamblia). PMID:20445943

  16. Modelling high-resolution electron microscopy based on core-loss spectroscopy

    International Nuclear Information System (INIS)

    Allen, L.J.; Findlay, S.D.; Oxley, M.P.; Witte, C.; Zaluzec, N.J.

    2006-01-01

    There are a number of factors affecting the formation of images based on core-loss spectroscopy in high-resolution electron microscopy. We demonstrate unambiguously the need to use a full nonlocal description of the effective core-loss interaction for experimental results obtained from high angular resolution electron channelling electron spectroscopy. The implications of this model are investigated for atomic resolution scanning transmission electron microscopy. Simulations are used to demonstrate that core-loss spectroscopy images formed using fine probes proposed for future microscopes can result in images that do not correspond visually with the structure that has led to their formation. In this context, we also examine the effect of varying detector geometries. The importance of the contribution to core-loss spectroscopy images by dechannelled or diffusely scattered electrons is reiterated here

  17. VEDA: a web-based virtual environment for dynamic atomic force microscopy.

    Science.gov (United States)

    Melcher, John; Hu, Shuiqing; Raman, Arvind

    2008-06-01

    We describe here the theory and applications of virtual environment dynamic atomic force microscopy (VEDA), a suite of state-of-the-art simulation tools deployed on nanoHUB (www.nanohub.org) for the accurate simulation of tip motion in dynamic atomic force microscopy (dAFM) over organic and inorganic samples. VEDA takes advantage of nanoHUB's cyberinfrastructure to run high-fidelity dAFM tip dynamics computations on local clusters and the teragrid. Consequently, these tools are freely accessible and the dAFM simulations are run using standard web-based browsers without requiring additional software. A wide range of issues in dAFM ranging from optimal probe choice, probe stability, and tip-sample interaction forces, power dissipation, to material property extraction and scanning dynamics over hetereogeneous samples can be addressed.

  18. Invited Article: VEDA: A web-based virtual environment for dynamic atomic force microscopy

    Science.gov (United States)

    Melcher, John; Hu, Shuiqing; Raman, Arvind

    2008-06-01

    We describe here the theory and applications of virtual environment dynamic atomic force microscopy (VEDA), a suite of state-of-the-art simulation tools deployed on nanoHUB (www.nanohub.org) for the accurate simulation of tip motion in dynamic atomic force microscopy (dAFM) over organic and inorganic samples. VEDA takes advantage of nanoHUB's cyberinfrastructure to run high-fidelity dAFM tip dynamics computations on local clusters and the teragrid. Consequently, these tools are freely accessible and the dAFM simulations are run using standard web-based browsers without requiring additional software. A wide range of issues in dAFM ranging from optimal probe choice, probe stability, and tip-sample interaction forces, power dissipation, to material property extraction and scanning dynamics over hetereogeneous samples can be addressed.

  19. Atomic Force Microscopy Based Nanorobotics Modelling, Simulation, Setup Building and Experiments

    CERN Document Server

    Xie, Hui; Régnier, Stéphane; Sitti, Metin

    2012-01-01

    The atomic force microscope (AFM) has been successfully used to perform nanorobotic manipulation operations on nanoscale entities such as particles, nanotubes, nanowires, nanocrystals, and DNA since 1990s. There have been many progress on modeling, imaging, teleoperated or automated control, human-machine interfacing, instrumentation, and applications of AFM based nanorobotic manipulation systems in literature. This book aims to include all of such state-of-the-art progress in an organized, structured, and detailed manner as a reference book and also potentially a textbook in nanorobotics and any other nanoscale dynamics, systems and controls related research and education. Clearly written and well-organized, this text introduces designs and prototypes of the nanorobotic systems in detail with innovative principles of three-dimensional manipulation force microscopy and parallel imaging/manipulation force microscopy.

  20. Parallel detecting super-resolution microscopy using correlation based image restoration

    Science.gov (United States)

    Yu, Zhongzhi; Liu, Shaocong; Zhu, Dazhao; Kuang, Cuifang; Liu, Xu

    2017-12-01

    A novel approach to achieve the image restoration is proposed in which each detector's relative position in the detector array is no longer a necessity. We can identify each detector's relative location by extracting a certain area from one of the detector's image and scanning it on other detectors' images. According to this location, we can generate the point spread functions (PSF) for each detector and perform deconvolution for image restoration. Equipped with this method, the microscope with discretionally designed detector array can be easily constructed without the concern of exact relative locations of detectors. The simulated results and experimental results show the total improvement in resolution with a factor of 1.7 compared to conventional confocal fluorescence microscopy. With the significant enhancement in resolution and easiness for application of this method, this novel method should have potential for a wide range of application in fluorescence microscopy based on parallel detecting.

  1. New directions in point-contact spectroscopy based on scanning tunneling microscopy techniques (Review Article)

    International Nuclear Information System (INIS)

    Tartaglini, E.; Verhagen, T.G.A.; Galli, F.; Trouwborst, M.L.; Aarts, J.; Van-Ruitebbeek, J.M.; Muller, R.; Shiota, T.

    2013-01-01

    Igor Yanson showed 38 years ago for the first time a point-contact measurement where he probed the energy resolved spectroscopy of the electronic scattering inside the metal. Since this first measurement, the pointcontact spectroscopy (PCS) technique improved enormously. The application of the scanning probe microscopy (SPM) techniques in the late 1980s allowed achieving contacts with a diameter of a single atom. With the introduction of the mechanically controlled break junction technique, even spectroscopy on freely suspended chains of atoms could be performed. In this paper, we briefly review the current developments of PCS and show recent experiments in advanced scanning PCS based on SPM techniques. We describe some results obtained with both needle-anvil type of point contacts and scanning tunneling microscopy (STM). We also show our first attempt to lift up with a STM a chain of single gold atoms from a Au(110) surface.

  2. Improved axial resolution of FINCH fluorescence microscopy when combined with spinning disk confocal microscopy.

    Science.gov (United States)

    Siegel, Nisan; Brooker, Gary

    2014-09-22

    FINCH holographic fluorescence microscopy creates super-resolved images with enhanced depth of focus. Addition of a Nipkow disk real-time confocal image scanner is shown to reduce the FINCH depth of focus while improving transverse confocal resolution in a combined method called "CINCH".

  3. Holographic complexity for time-dependent backgrounds

    Energy Technology Data Exchange (ETDEWEB)

    Momeni, Davood, E-mail: davoodmomeni78@gmail.com [Eurasian International Center for Theoretical Physics and Department of General Theoretical Physics, Eurasian National University, Astana 010008 (Kazakhstan); Faizal, Mir, E-mail: mirfaizalmir@googlemail.com [Irving K. Barber School of Arts and Sciences, University of British Columbia, Okanagan, 3333 University Way, Kelowna, British Columbia V1V 1V7 (Canada); Department of Physics and Astronomy, University of Lethbridge, Lethbridge, Alberta, T1K 3M4 (Canada); Bahamonde, Sebastian, E-mail: sebastian.beltran.14@ucl.ac.uk [Department of Mathematics, University College London, Gower Street, London, WC1E 6BT (United Kingdom); Myrzakulov, Ratbay [Eurasian International Center for Theoretical Physics and Department of General Theoretical Physics, Eurasian National University, Astana 010008 (Kazakhstan)

    2016-11-10

    In this paper, we will analyze the holographic complexity for time-dependent asymptotically AdS geometries. We will first use a covariant zero mean curvature slicing of the time-dependent bulk geometries, and then use this co-dimension one spacelike slice of the bulk spacetime to define a co-dimension two minimal surface. The time-dependent holographic complexity will be defined using the volume enclosed by this minimal surface. This time-dependent holographic complexity will reduce to the usual holographic complexity for static geometries. We will analyze the time-dependence as a perturbation of the asymptotically AdS geometries. Thus, we will obtain time-dependent asymptotically AdS geometries, and we will calculate the holographic complexity for such time-dependent geometries.

  4. Atomic force microscopy-based repeated machining theory for nanochannels on silicon oxide surfaces

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Z.Q., E-mail: wangzhiqian@sia.cn [State Key Laboratory of Robotics, Shenyang Institute of Automation, CAS, Shenyang 110016 (China); Graduate University of the Chinese Academy of Sciences, Beijing 100049 (China); Jiao, N.D. [State Key Laboratory of Robotics, Shenyang Institute of Automation, CAS, Shenyang 110016 (China); Tung, S. [Department of Mechanical Engineering, University of Arkansas, Fayetteville, AR 72701 (United States); Dong, Z.L. [State Key Laboratory of Robotics, Shenyang Institute of Automation, CAS, Shenyang 110016 (China)

    2011-02-01

    The atomic force microscopy (AFM)-based repeated nanomachining of nanochannels on silicon oxide surfaces is investigated both theoretically and experimentally. The relationships of the initial nanochannel depth vs. final nanochannel depth at a normal force are systematically studied. Using the derived theory and simulation results, the final nanochannel depth can be predicted easily. Meanwhile, if a nanochannel with an expected depth needs to be machined, a right normal force can be selected simply and easily in order to decrease the wear of the AFM tip. The theoretical analysis and simulation results can be effectively used for AFM-based fabrication of nanochannels.

  5. Asymmetric actuating structure generates negligible influence on the supporting base for high performance scanning probe microscopies

    Science.gov (United States)

    Yi Yan, Gang; Bin Liu, Yong; Hua Feng, Zhi

    2014-02-01

    An asymmetric actuating structure generating negligible influence on the supporting base for high performance scanning probe microscopies is proposed in this paper. The actuator structure consists of two piezostacks, one is used for actuating while the other is for counterbalancing. In contrast with balanced structure, the two piezostacks are installed at the same side of the supporting base. The effectiveness of the structure is proved by some experiments with the actuators fixed to the free end of a cantilever. Experimental results show that almost all of the vibration modes of the cantilever are suppressed effectively at a wide frequency range of 90 Hz-10 kHz.

  6. Resolution enhancement of holographic printer using a hogel overlapping method.

    Science.gov (United States)

    Hong, Keehoon; Park, Soon-gi; Yeom, Jiwoon; Kim, Jonghyun; Chen, Ni; Pyun, Kyungsuk; Choi, Chilsung; Kim, Sunil; An, Jungkwuen; Lee, Hong-Seok; Chung, U-in; Lee, Byoungho

    2013-06-17

    We propose a hogel overlapping method for the holographic printer to enhance the lateral resolution of holographic stereograms. The hogel size is directly related to the lateral resolution of the holographic stereogram. Our analysis by computer simulation shows that there is a limit to decreasing the hogel size while printing holographic stereograms. Instead of reducing the size of hogel, the lateral resolution of holographic stereograms can be enhanced by printing overlapped hogels, which makes it possible to take advantage of multiplexing property of the volume hologram. We built a holographic printer, and recorded two holographic stereograms using the conventional and proposed overlapping methods. The images and movies of the holographic stereograms experimentally captured were compared between the conventional and proposed methods. The experimental results confirm that the proposed hogel overlapping method improves the lateral resolution of holographic stereograms compared to the conventional holographic printing method.

  7. Holographic considerations on a Machian Universe

    Energy Technology Data Exchange (ETDEWEB)

    Abreu, Everton M.C., E-mail: evertonabreu@ufrrj.br [Grupo de Física Teórica e Matemática Física, Departamento de Física, Universidade Federal Rural do Rio de Janeiro, BR 465-07, 23890-971, Seropédica, RJ (Brazil); Departamento de Física, ICE, Universidade Federal de Juiz de Fora, 36036-330, Juiz de Fora, MG (Brazil); Ananias Neto, Jorge, E-mail: jorge@fisica.ufjf.br [Departamento de Física, ICE, Universidade Federal de Juiz de Fora, 36036-330, Juiz de Fora, MG (Brazil)

    2014-12-15

    MOND theory explains the rotation curves of the galaxies. Verlinde’s ideas establish an entropic origin for gravitational forces and Tsallis principle generalizes the theory of Boltzmann–Gibbs. In this work we have promoted a connection between these recent approaches, that at first sight seemed to have few or no points in common, using the Mach’s principle as the background. In this way we have used Tsallis formalism to calculate the main parameters of the Machian Universe including the Hubble parameter and the age of the Universe. After that, we have also obtained a new value for the Tsallis parameter via Mach’s principle. Using Verlinde’s entropic gravity we have obtained new forms for MOND’s well established ingredients. Finally, based on the relations between particles and bits obtained here, we have discussed the idea of bits entanglement in the holographic screen.

  8. Supersymmetric null-like holographic cosmologies

    International Nuclear Information System (INIS)

    Lin Fengli; Wen Wenyu

    2006-01-01

    We construct a new class of 1/4-BPS time dependent domain-wall solutions with null-like metric and dilaton in type II supergravities, which admit a null-like big bang singularity. Based on the domain-wall/QFT correspondence, these solutions are dual to 1/4-supersymmetric quantum field theories living on a boundary cosmological background with time dependent coupling constant and UV cutoff. In particular we evaluate the holographic c function for the 2-dimensional dual field theory living on the corresponding null-like cosmology. We find that this c function runs in accordance with the c-theorem as the boundary universe evolves, this means that the number of degrees of freedom is divergent at big bang and suggests the possible resolution of big bang singularity

  9. Concepts in Light Microscopy of Viruses

    Science.gov (United States)

    Witte, Robert; Georgi, Fanny

    2018-01-01

    Viruses threaten humans, livestock, and plants, and are difficult to combat. Imaging of viruses by light microscopy is key to uncover the nature of known and emerging viruses in the quest for finding new ways to treat viral disease and deepening the understanding of virus–host interactions. Here, we provide an overview of recent technology for imaging cells and viruses by light microscopy, in particular fluorescence microscopy in static and live-cell modes. The review lays out guidelines for how novel fluorescent chemical probes and proteins can be used in light microscopy to illuminate cells, and how they can be used to study virus infections. We discuss advantages and opportunities of confocal and multi-photon microscopy, selective plane illumination microscopy, and super-resolution microscopy. We emphasize the prevalent concepts in image processing and data analyses, and provide an outlook into label-free digital holographic microscopy for virus research. PMID:29670029

  10. Time-stretch microscopy based on time-wavelength sequence reconstruction from wideband incoherent source

    International Nuclear Information System (INIS)

    Zhang, Chi; Xu, Yiqing; Wei, Xiaoming; Tsia, Kevin K.; Wong, Kenneth K. Y.

    2014-01-01

    Time-stretch microscopy has emerged as an ultrafast optical imaging concept offering the unprecedented combination of the imaging speed and sensitivity. However, dedicated wideband and coherence optical pulse source with high shot-to-shot stability has been mandated for time-wavelength mapping—the enabling process for ultrahigh speed wavelength-encoded image retrieval. From the practical point of view, exploiting methods to relax the stringent requirements (e.g., temporal stability and coherence) for the source of time-stretch microscopy is thus of great value. In this paper, we demonstrated time-stretch microscopy by reconstructing the time-wavelength mapping sequence from a wideband incoherent source. Utilizing the time-lens focusing mechanism mediated by a narrow-band pulse source, this approach allows generation of a wideband incoherent source, with the spectral efficiency enhanced by a factor of 18. As a proof-of-principle demonstration, time-stretch imaging with the scan rate as high as MHz and diffraction-limited resolution is achieved based on the wideband incoherent source. We note that the concept of time-wavelength sequence reconstruction from wideband incoherent source can also be generalized to any high-speed optical real-time measurements, where wavelength is acted as the information carrier

  11. Experimental research of digital holographic microscopic measuring

    Science.gov (United States)

    Zhu, Xueliang; Chen, Feifei; Li, Jicheng

    2013-06-01

    Digital holography is a new imaging technique, which is developed on the base of optical holography, Digital processing, and Computer techniques. It is using CCD instead of the conventional silver to record hologram, and then reproducing the 3D contour of the object by the way of computer simulation. Compared with the traditional optical holographic, the whole process is of simple measuring, lower production cost, faster the imaging speed, and with the advantages of non-contact real-time measurement. At present, it can be used in the fields of the morphology detection of tiny objects, micro deformation analysis, and biological cells shape measurement. It is one of the research hot spot at home and abroad. This paper introduced the basic principles and relevant theories about the optical holography and Digital holography, and researched the basic questions which influence the reproduce images in the process of recording and reconstructing of the digital holographic microcopy. In order to get a clear digital hologram, by analyzing the optical system structure, we discussed the recording distance and of the hologram. On the base of the theoretical studies, we established a measurement and analyzed the experimental conditions, then adjusted them to the system. To achieve a precise measurement of tiny object in three-dimension, we measured MEMS micro device for example, and obtained the reproduction three-dimensional contour, realized the three dimensional profile measurement of tiny object. According to the experiment results consider: analysis the reference factors between the zero-order term and a pair of twin-images by the choice of the object light and the reference light and the distance of the recording and reconstructing and the characteristics of reconstruction light on the measurement, the measurement errors were analyzed. The research result shows that the device owns certain reliability.

  12. Improving your four-dimensional image: traveling through a decade of light-sheet-based fluorescence microscopy research.

    Science.gov (United States)

    Strobl, Frederic; Schmitz, Alexander; Stelzer, Ernst H K

    2017-06-01

    Light-sheet-based fluorescence microscopy features optical sectioning in the excitation process. This reduces phototoxicity and photobleaching by up to four orders of magnitude compared with that caused by confocal fluorescence microscopy, simplifies segmentation and quantification for three-dimensional cell biology, and supports the transition from on-demand to systematic data acquisition in developmental biology applications.

  13. Progress and prospects in soft x-ray holographic microscopy

    International Nuclear Information System (INIS)

    Howells, M.R.; Jacobsen, C.; Kirz, J.; McQuaid, K.; Rothman, S.S.

    1987-12-01

    We report some of the latest developments in x-ray holography experiments and make some speculations about the limits of performance of the approaches currently in use. We also make some suggestions about where the technique can (and cannot) go in the future. 32 refs., 5 figs., 1 tab

  14. Fourth sound of holographic superfluids

    International Nuclear Information System (INIS)

    Yarom, Amos

    2009-01-01

    We compute fourth sound for superfluids dual to a charged scalar and a gauge field in an AdS 4 background. For holographic superfluids with condensates that have a large scaling dimension (greater than approximately two), we find that fourth sound approaches first sound at low temperatures. For condensates that a have a small scaling dimension it exhibits non-conformal behavior at low temperatures which may be tied to the non-conformal behavior of the order parameter of the superfluid. We show that by introducing an appropriate scalar potential, conformal invariance can be enforced at low temperatures.

  15. A novel multiphoton microscopy images segmentation method based on superpixel and watershed.

    Science.gov (United States)

    Wu, Weilin; Lin, Jinyong; Wang, Shu; Li, Yan; Liu, Mingyu; Liu, Gaoqiang; Cai, Jianyong; Chen, Guannan; Chen, Rong

    2017-04-01

    Multiphoton microscopy (MPM) imaging technique based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) shows fantastic performance for biological imaging. The automatic segmentation of cellular architectural properties for biomedical diagnosis based on MPM images is still a challenging issue. A novel multiphoton microscopy images segmentation method based on superpixels and watershed (MSW) is presented here to provide good segmentation results for MPM images. The proposed method uses SLIC superpixels instead of pixels to analyze MPM images for the first time. The superpixels segmentation based on a new distance metric combined with spatial, CIE Lab color space and phase congruency features, divides the images into patches which keep the details of the cell boundaries. Then the superpixels are used to reconstruct new images by defining an average value of superpixels as image pixels intensity level. Finally, the marker-controlled watershed is utilized to segment the cell boundaries from the reconstructed images. Experimental results show that cellular boundaries can be extracted from MPM images by MSW with higher accuracy and robustness. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  16. Lectures on holographic methods for condensed matter physics

    International Nuclear Information System (INIS)

    Hartnoll, Sean A

    2009-01-01

    These notes are loosely based on lectures given at the CERN Winter School on Supergravity, Strings and Gauge theories, February 2009, and at the IPM String School in Tehran, April 2009. I have focused on a few concrete topics and also on addressing questions that have arisen repeatedly. Background condensed matter physics material is included as motivation and easy reference for the high energy physics community. The discussion of holographic techniques progresses from equilibrium, to transport and to superconductivity.

  17. Nanograting-based plasmon enhancement for total internal reflection fluorescence microscopy of live cells

    International Nuclear Information System (INIS)

    Kim, Kyujung; Cho, Eun-Jin; Suh, Jin-Suck; Huh, Yong-Min; Kim, Donghyun; Kim, Dong Jun

    2009-01-01

    We investigated evanescent field enhancement based on subwavelength nanogratings for improved sensitivity in total internal reflection microscopy of live cells. The field enhancement is associated with subwavelength-grating-coupled plasmon excitation. An optimum sample employed a silver grating on a silver film and an SF10 glass substrate. Field intensity was enhanced by approximately 90% when measured by fluorescent excitation of microbeads relative to that on a bare prism as a control, which is in good agreement with numerical results. The subwavelength-grating-mediated field enhancement was also applied to live cell imaging of quantum dots, which confirmed the sensitivity enhancement qualitatively.

  18. Single particle analysis based on Zernike phase contrast transmission electron microscopy.

    Science.gov (United States)

    Danev, Radostin; Nagayama, Kuniaki

    2008-02-01

    We present the first application of Zernike phase-contrast transmission electron microscopy to single-particle 3D reconstruction of a protein, using GroEL chaperonin as the test specimen. We evaluated the performance of the technique by comparing 3D models derived from Zernike phase contrast imaging, with models from conventional underfocus phase contrast imaging. The same resolution, about 12A, was achieved by both imaging methods. The reconstruction based on Zernike phase contrast data required about 30% fewer particles. The advantages and prospects of each technique are discussed.

  19. Moving through a multiplex holographic scene

    Science.gov (United States)

    Mrongovius, Martina

    2013-02-01

    This paper explores how movement can be used as a compositional element in installations of multiplex holograms. My holographic images are created from montages of hand-held video and photo-sequences. These spatially dynamic compositions are visually complex but anchored to landmarks and hints of the capturing process - such as the appearance of the photographer's shadow - to establish a sense of connection to the holographic scene. Moving around in front of the hologram, the viewer animates the holographic scene. A perception of motion then results from the viewer's bodily awareness of physical motion and the visual reading of dynamics within the scene or movement of perspective through a virtual suggestion of space. By linking and transforming the physical motion of the viewer with the visual animation, the viewer's bodily awareness - including proprioception, balance and orientation - play into the holographic composition. How multiplex holography can be a tool for exploring coupled, cross-referenced and transformed perceptions of movement is demonstrated with a number of holographic image installations. Through this process I expanded my creative composition practice to consider how dynamic and spatial scenes can be conveyed through the fragmented view of a multiplex hologram. This body of work was developed through an installation art practice and was the basis of my recently completed doctoral thesis: 'The Emergent Holographic Scene — compositions of movement and affect using multiplex holographic images'.

  20. Silver nanowires for highly reproducible cantilever based AFM-TERS microscopy: towards a universal TERS probe.

    Science.gov (United States)

    Walke, Peter; Fujita, Yasuhiko; Peeters, Wannes; Toyouchi, Shuichi; Frederickx, Wout; De Feyter, Steven; Uji-I, Hiroshi

    2018-04-26

    Tip-enhanced Raman scattering (TERS) microscopy is a unique analytical tool to provide complementary chemical and topographic information of surfaces with nanometric resolution. However, difficulties in reliably producing the necessary metallized scanning probe tips has limited its widespread utilisation, particularly in the case of cantilever-based atomic force microscopy. Attempts to alleviate tip related issues using colloidal or bottom-up engineered tips have so far not reported consistent probes for both Raman and topographic imaging. Here we demonstrate the reproducible fabrication of cantilever-based high-performance TERS probes for both topographic and Raman measurements, based on an approach that utilises noble metal nanowires as the active TERS probe. The tips show 10 times higher TERS contrasts than the most typically used electrochemically-etched tips, and show a reproducibility for TERS greater than 90%, far greater than found with standard methods. We show that TERS can be performed in tapping as well as contact AFM mode, with optical resolutions around or below 15 nm, and with a maximum resolution achieved in tapping-mode of 6 nm. Our work illustrates that superior TERS probes can be produced in a fast and cost-effective manner using simple wet-chemistry methods, leading to reliable and reproducible high-resolution and high-sensitivity TERS, and thus renders the technique applicable for a broad community.

  1. A Virtual Holographic Display Case for Museum Installations

    Directory of Open Access Journals (Sweden)

    Manuela Chessa

    2015-12-01

    Full Text Available Today, it is important in society to make artworks accessible to mass audiences and to widen participation in culture. In such a context, virtual reality is one of the areas of greatest interest: new devices and new techniques are affordable for many users, and virtual and real worlds are often mixed together. In this paper, we propose a "virtual holographic" display, i.e. a stereoscopic virtual reality system that is able to replicate the behavior of a real showcase for exhibitions. It works in a completely virtual manner and it can yield to a new generation of entertainment "holographic" installations. We evaluate such a system through an experimental session with 20 users. In particular, we compare the proposed system, based on a stereoscopic technique (TD3D, with respect to a standard motion parallax technique in terms of the users' perceptual experience.

  2. Read-only high accuracy volume holographic optical correlator

    Science.gov (United States)

    Zhao, Tian; Li, Jingming; Cao, Liangcai; He, Qingsheng; Jin, Guofan

    2011-10-01

    A read-only volume holographic correlator (VHC) is proposed. After the recording of all of the correlation database pages by angular multiplexing, a stand-alone read-only high accuracy VHC will be separated from the VHC recording facilities which include the high-power laser and the angular multiplexing system. The stand-alone VHC has its own low power readout laser and very compact and simple structure. Since there are two lasers that are employed for recording and readout, respectively, the optical alignment tolerance of the laser illumination on the SLM is very sensitive. The twodimensional angular tolerance is analyzed based on the theoretical model of the volume holographic correlator. The experimental demonstration of the proposed read-only VHC is introduced and discussed.

  3. Application of holographic elements in displays and planar illuminators

    Science.gov (United States)

    Putilin, Andrew; Gustomiasov, Igor

    2007-05-01

    Holographic Optical Elements (HOE's) on planar waveguides can be used to design the planar optics for backlit units, color selectors or filters, lenses for virtual reality displays. The several schemes for HOE recording are proposed to obtain planar stereo backlit unit and private eye displays light source. It is shown in the paper that the specific light transformation grating permits to construct efficient backlit units for display holograms and LCD. Several schemes of reflection/transmission backlit units and scattering films based on holographic optical elements are also proposed. The performance of the waveguide HOE can be optimized using the parameters of recording scheme and etching parameters. The schemes of HOE application are discussed and some experimental results are shown.

  4. Exploring holographic Composite Higgs models

    Energy Technology Data Exchange (ETDEWEB)

    Croon, Djuna [Department of Physics and Astronomy, University of Sussex,BN1 9QH Brighton (United Kingdom); Perimeter Institute for Theoretical Physics,Waterloo, ON (Canada); Dillon, Barry M.; Huber, Stephan J.; Sanz, Veronica [Department of Physics and Astronomy, University of Sussex,BN1 9QH Brighton (United Kingdom)

    2016-07-13

    Simple Composite Higgs models predict new vector-like fermions not too far from the electroweak scale, yet LHC limits are now sensitive to the TeV scale. Motivated by this tension, we explore the holographic dual of the minimal model, MCHM{sub 5}, to try and alleviate this tension without increasing the fine-tuning in the Higgs potential. Interestingly, we find that lowering the UV cutoff in the 5D picture allows for heavier top partners and less fine-tuning. In the 4D dual this corresponds to increasing the number of “colours” N, thus increasing the decay constant of the Goldstone Higgs. This is essentially a ‘Little Randall-Sundrum Model’, which are known to reduce some flavour and electroweak constraints. Furthermore, in anticipation of the ongoing efforts at the LHC to put bounds on the top Yukawa, we demonstrate that deviations from the SM can be suppressed or enhanced with respect to what is expected from mere symmetry arguments in 4D. We conclude that the 5D holographic realisation of the MCHM{sub 5} with a small UV cutoff is not in tension with the current experimental data.

  5. Fidelity susceptibility as holographic PV-criticality

    Energy Technology Data Exchange (ETDEWEB)

    Momeni, Davood, E-mail: davoodmomeni78@gmail.com [Eurasian International Center for Theoretical Physics and Department of General & Theoretical Physics, Eurasian National University, Astana 010008 (Kazakhstan); Faizal, Mir, E-mail: mirfaizalmir@googlemail.com [Department of Physics and Astronomy, University of Lethbridge, Lethbridge, Alberta T1K 3M4 (Canada); Irving K. Barber School of Arts and Sciences, University of British Columbia – Okanagan, 3333 University Way, Kelowna, British Columbia V1V 1V7 (Canada); Myrzakulov, Kairat, E-mail: kairatmyrzakul@gmail.com [Eurasian International Center for Theoretical Physics and Department of General & Theoretical Physics, Eurasian National University, Astana 010008 (Kazakhstan); Myrzakulov, Ratbay, E-mail: rmyrzakulov@gmail.com [Eurasian International Center for Theoretical Physics and Department of General & Theoretical Physics, Eurasian National University, Astana 010008 (Kazakhstan)

    2017-02-10

    It is well known that entropy can be used to holographically establish a connection among geometry, thermodynamics and information theory. In this paper, we will use complexity to holographically establish a connection among geometry, thermodynamics and information theory. Thus, we will analyze the relation among holographic complexity, fidelity susceptibility, and thermodynamics in extended phase space. We will demonstrate that fidelity susceptibility (which is the informational complexity dual to a maximum volume in AdS) can be related to the thermodynamical volume (which is conjugate to the cosmological constant in the extended thermodynamic phase space). Thus, this letter establishes a relation among geometry, thermodynamics, and information theory, using complexity.

  6. Study of crystalline thin films and nanofibers by means of the laser–plasma EUV-source based microscopy

    International Nuclear Information System (INIS)

    Wachulak, P.W.; Bartnik, A.; Baranowska-Korczyc, A.; Pánek, D.; Brůža, P.; Kostecki, J.; Węgrzyński, Ł.; Jarocki, R.; Szczurek, M.; Fronc, K.; Elbaum, D.; Fiedorowicz, H.

    2013-01-01

    New developments in nanoscience and nanotechnology require nanometer scale resolution imaging tools and techniques such as an extreme ultraviolet (EUV) and soft X-ray (SXR) microscopy, based on Fresnel zone plates. In this paper, we report on applications of a desk-top microscopy using a laser-plasma EUV source based on a gas-puff target for studies of morphology of thin silicon membranes coated with NaCl crystals and samples composed of ZnO nanofibers

  7. Analysis of Point Based Image Registration Errors With Applications in Single Molecule Microscopy.

    Science.gov (United States)

    Cohen, E A K; Ober, R J

    2013-12-15

    We present an asymptotic treatment of errors involved in point-based image registration where control point (CP) localization is subject to heteroscedastic noise; a suitable model for image registration in fluorescence microscopy. Assuming an affine transform, CPs are used to solve a multivariate regression problem. With measurement errors existing for both sets of CPs this is an errors-in-variable problem and linear least squares is inappropriate; the correct method being generalized least squares. To allow for point dependent errors the equivalence of a generalized maximum likelihood and heteroscedastic generalized least squares model is achieved allowing previously published asymptotic results to be extended to image registration. For a particularly useful model of heteroscedastic noise where covariance matrices are scalar multiples of a known matrix (including the case where covariance matrices are multiples of the identity) we provide closed form solutions to estimators and derive their distribution. We consider the target registration error (TRE) and define a new measure called the localization registration error (LRE) believed to be useful, especially in microscopy registration experiments. Assuming Gaussianity of the CP localization errors, it is shown that the asymptotic distribution for the TRE and LRE are themselves Gaussian and the parameterized distributions are derived. Results are successfully applied to registration in single molecule microscopy to derive the key dependence of the TRE and LRE variance on the number of CPs and their associated photon counts. Simulations show asymptotic results are robust for low CP numbers and non-Gaussianity. The method presented here is shown to outperform GLS on real imaging data.

  8. Photon-counting-based diffraction phase microscopy combined with single-pixel imaging

    Science.gov (United States)

    Shibuya, Kyuki; Araki, Hiroyuki; Iwata, Tetsuo

    2018-04-01

    We propose a photon-counting (PC)-based quantitative-phase imaging (QPI) method for use in diffraction phase microscopy (DPM) that is combined with a single-pixel imaging (SPI) scheme (PC-SPI-DPM). This combination of DPM with the SPI scheme overcomes a low optical throughput problem that has occasionally prevented us from obtaining quantitative-phase images in DPM through use of a high-sensitivity single-channel photodetector such as a photomultiplier tube (PMT). The introduction of a PMT allowed us to perform PC with ease and thus solved a dynamic range problem that was inherent to SPI. As a proof-of-principle experiment, we performed a comparison study of analogue-based SPI-DPM and PC-SPI-DPM for a 125-nm-thick indium tin oxide (ITO) layer coated on a silica glass substrate. We discuss the basic performance of the method and potential future modifications of the proposed system.

  9. Electrical tomography using atomic force microscopy and its application towards carbon nanotube-based interconnects

    International Nuclear Information System (INIS)

    Schulze, A; Hantschel, T; Dathe, A; Eyben, P; Vandervorst, W; Ke, X

    2012-01-01

    The fabrication and integration of low-resistance carbon nanotubes (CNTs) for interconnects in future integrated circuits requires characterization techniques providing structural and electrical information at the nanometer scale. In this paper we present a slice-and-view approach based on electrical atomic force microscopy. Material removal achieved by successive scanning using doped ultra-sharp full-diamond probes, manufactured in-house, enables us to acquire two-dimensional (2D) resistance maps originating from different depths (equivalently different CNT lengths) on CNT-based interconnects. Stacking and interpolating these 2D resistance maps results in a three-dimensional (3D) representation (tomogram). This allows insight from a structural (e.g. size, density, distribution, straightness) and electrical point of view simultaneously. By extracting the resistance evolution over the length of an individual CNT we derive quantitative information about the resistivity and the contact resistance between the CNT and bottom electrode. (paper)

  10. Characterization of LiF-based soft X-ray imaging detectors by confocal fluorescence microscopy

    International Nuclear Information System (INIS)

    Bonfigli, F; Gaudio, P; Lupelli, I; Nichelatti, E; Richetta, M; Vincenti, M A; Montereali, R M

    2010-01-01

    X-ray microscopy represents a powerful tool to obtain images of samples with very high spatial resolution. The main limitation of this technique is represented by the poor spatial resolution of standard imaging detectors. We proposed an innovative high-performance X-ray imaging detector based on the visible photoluminescence of colour centres in lithium fluoride. In this work, a confocal microscope in fluorescence mode was used to characterize LiF-based imaging detectors measuring CC integrated visible fluorescence signals of LiF crystals and films (grown on several kinds of substrates) irradiated by soft X-rays produced by a laser plasma source in different exposure conditions. The results are compared with the CC photoluminescence spectra measured on the same samples and discussed.

  11. Atomic-resolution single-spin magnetic resonance detection concept based on tunneling force microscopy

    Science.gov (United States)

    Payne, A.; Ambal, K.; Boehme, C.; Williams, C. C.

    2015-05-01

    A study of a force detected single-spin magnetic resonance measurement concept with atomic spatial resolution is presented. The method is based upon electrostatic force detection of spin-selection rule controlled single-electron tunneling between two electrically isolated paramagnetic states. Single-spin magnetic resonance detection is possible by measuring the force detected tunneling charge noise on and off spin resonance. Simulation results of this charge noise, based upon physical models of the tunneling and spin physics, are directly compared to measured atomic force microscopy system noise. The results show that the approach could provide single-spin measurement of electrically isolated qubit states with atomic spatial resolution at room temperature.

  12. Examining live cell cultures during apoptosis by digital holographic phase imaging and Raman spectroscopy

    Science.gov (United States)

    Khmaladze, Alexander

    2017-11-01

    Cellular apoptosis is a unique, organized series of events, leading to programmed cell death. In this work, we present a combined digital holography/Raman spectroscopy technique to study live cell cultures during apoptosis. Digital holographic microscopy measurements of live cell cultures yield information about cell shape and volume, changes to which are indicative of alterations in cell cycle and initiation of cell death mechanisms. Raman spectroscopic measurements provide complementary information about cells, such as protein, lipid and nucleic acid content, and the spectral signatures associated with structural changes in molecules. Our work indicates that the chemical changes in proteins, which were detected by Raman measurements, preceded morphological changes, which were seen with digital holographic microscopy.

  13. Some applications of holographic interferometry in biomechanics

    Science.gov (United States)

    Ebbeni, Jean P. L.

    1992-03-01

    Holographic interferometry is well adapted for the determination of 2D strain fields in osseous structures. The knowledge of those strain fields is important for the understanding of structure behavior such as arthrosis.

  14. Holographic equipartition from first order action

    Science.gov (United States)

    Wang, Jingbo

    2017-12-01

    Recently, the idea that gravity is emergent has attract many people's attention. The "Emergent Gravity Paradigm" is a program that develop this idea from the thermodynamical point of view. It expresses the Einstein equation in the language of thermodynamics. A key equation in this paradigm is the holographic equipartition which says that, in all static spacetimes, the degrees of freedom on the boundary equal those in the bulk. And the time evolution of spacetime is drove by the departure from the holographic equipartition. In this paper, we get the holographic equipartition and its generalization from the first order formalism, that is, the connection and its conjugate momentum are considered to be the canonical variables. The final results have similar structure as those from the metric formalism. It gives another proof of holographic equipartition.

  15. Entanglement entropy and complexity for one-dimensional holographic superconductors

    Science.gov (United States)

    Kord Zangeneh, Mahdi; Ong, Yen Chin; Wang, Bin

    2017-08-01

    Holographic superconductor is an important arena for holography, as it allows concrete calculations to further understand the dictionary between bulk physics and boundary physics. An important quantity of recent interest is the holographic complexity. Conflicting claims had been made in the literature concerning the behavior of holographic complexity during phase transition. We clarify this issue by performing a numerical study on one-dimensional holographic superconductor. Our investigation shows that holographic complexity does not behave in the same way as holographic entanglement entropy. Nevertheless, the universal terms of both quantities are finite and reflect the phase transition at the same critical temperature.

  16. Isometric multimodal photoacoustic microscopy based on optically transparent micro-ring ultrasonic detection.

    Science.gov (United States)

    Dong, Biqin; Li, Hao; Zhang, Zhen; Zhang, Kevin; Chen, Siyu; Sun, Cheng; Zhang, Hao F

    2015-01-01

    Photoacoustic microscopy (PAM) is an attractive imaging tool complementary to established optical microscopic modalities by providing additional molecular specificities through imaging optical absorption contrast. While the development of optical resolution photoacoustic microscopy (ORPAM) offers high lateral resolution, the acoustically-determined axial resolution is limited due to the constraint in ultrasonic detection bandwidth. ORPAM with isometric spatial resolution along both axial and lateral direction is yet to be developed. Although recently developed sophisticated optical illumination and reconstruction methods offer improved axial resolution in ORPAM, the image acquisition procedures are rather complicated, limiting their capabilities for high-speed imaging and being easily integrated with established optical microscopic modalities. Here we report an isometric ORPAM based on an optically transparent micro-ring resonator ultrasonic detector and a commercial inverted microscope platform. Owing to the superior spatial resolution and the ease of integrating our ORPAM with established microscopic modalities, single cell imaging with extrinsic fluorescence staining, intrinsic autofluorescence, and optical absorption can be achieved simultaneously. This technique holds promise to greatly improve the accessibility of PAM to the broader biomedical researchers.

  17. A fast global fitting algorithm for fluorescence lifetime imaging microscopy based on image segmentation.

    Science.gov (United States)

    Pelet, S; Previte, M J R; Laiho, L H; So, P T C

    2004-10-01

    Global fitting algorithms have been shown to improve effectively the accuracy and precision of the analysis of fluorescence lifetime imaging microscopy data. Global analysis performs better than unconstrained data fitting when prior information exists, such as the spatial invariance of the lifetimes of individual fluorescent species. The highly coupled nature of global analysis often results in a significantly slower convergence of the data fitting algorithm as compared with unconstrained analysis. Convergence speed can be greatly accelerated by providing appropriate initial guesses. Realizing that the image morphology often correlates with fluorophore distribution, a global fitting algorithm has been developed to assign initial guesses throughout an image based on a segmentation analysis. This algorithm was tested on both simulated data sets and time-domain lifetime measurements. We have successfully measured fluorophore distribution in fibroblasts stained with Hoechst and calcein. This method further allows second harmonic generation from collagen and elastin autofluorescence to be differentiated in fluorescence lifetime imaging microscopy images of ex vivo human skin. On our experimental measurement, this algorithm increased convergence speed by over two orders of magnitude and achieved significantly better fits. Copyright 2004 Biophysical Society

  18. MISTICA: Minimum Spanning Tree-Based Coarse Image Alignment for Microscopy Image Sequences.

    Science.gov (United States)

    Ray, Nilanjan; McArdle, Sara; Ley, Klaus; Acton, Scott T

    2016-11-01

    Registration of an in vivo microscopy image sequence is necessary in many significant studies, including studies of atherosclerosis in large arteries and the heart. Significant cardiac and respiratory motion of the living subject, occasional spells of focal plane changes, drift in the field of view, and long image sequences are the principal roadblocks. The first step in such a registration process is the removal of translational and rotational motion. Next, a deformable registration can be performed. The focus of our study here is to remove the translation and/or rigid body motion that we refer to here as coarse alignment. The existing techniques for coarse alignment are unable to accommodate long sequences often consisting of periods of poor quality images (as quantified by a suitable perceptual measure). Many existing methods require the user to select an anchor image to which other images are registered. We propose a novel method for coarse image sequence alignment based on minimum weighted spanning trees (MISTICA) that overcomes these difficulties. The principal idea behind MISTICA is to reorder the images in shorter sequences, to demote nonconforming or poor quality images in the registration process, and to mitigate the error propagation. The anchor image is selected automatically making MISTICA completely automated. MISTICA is computationally efficient. It has a single tuning parameter that determines graph width, which can also be eliminated by the way of additional computation. MISTICA outperforms existing alignment methods when applied to microscopy image sequences of mouse arteries.

  19. An atomic force microscopy-based method for line edge roughness measurement

    Energy Technology Data Exchange (ETDEWEB)

    Fouchier, M.; Pargon, E.; Bardet, B. [CNRS/UJF-Grenoble1/CEA LTM, 17 avenue des Martyrs, 38054 Grenoble cedex 9 (France)

    2013-03-14

    With the constant decrease of semiconductor device dimensions, line edge roughness (LER) becomes one of the most important sources of device variability and needs to be controlled below 2 nm for the future technological nodes of the semiconductor roadmap. LER control at the nanometer scale requires accurate measurements. We introduce a technique for LER measurement based upon the atomic force microscope (AFM). In this technique, the sample is tilted at about 45 Degree-Sign and feature sidewalls are scanned along their length with the AFM tip to obtain three-dimensional images. The small radius of curvature of the tip together with the low noise level of a laboratory AFM result in high resolution images. Half profiles and LER values on all the height of the sidewalls are extracted from the 3D images using a procedure that we developed. The influence of sample angle variations on the measurements is shown to be small. The technique is applied to the study of a full pattern transfer into a simplified gate stack. The images obtained are qualitatively consistent with cross-section scanning electron microscopy images and the average LER values agree with that obtained by critical dimension scanning electron microscopy. In addition to its high resolution, this technique presents several advantages such as the ability to image the foot of photoresist lines, complex multi-layer stacks regardless of the materials, and deep re-entrant profiles.

  20. PScan 1.0: flexible software framework for polygon based multiphoton microscopy

    Science.gov (United States)

    Li, Yongxiao; Lee, Woei Ming

    2016-12-01

    Multiphoton laser scanning microscopes exhibit highly localized nonlinear optical excitation and are powerful instruments for in-vivo deep tissue imaging. Customized multiphoton microscopy has a significantly superior performance for in-vivo imaging because of precise control over the scanning and detection system. To date, there have been several flexible software platforms catered to custom built microscopy systems i.e. ScanImage, HelioScan, MicroManager, that perform at imaging speeds of 30-100fps. In this paper, we describe a flexible software framework for high speed imaging systems capable of operating from 5 fps to 1600 fps. The software is based on the MATLAB image processing toolbox. It has the capability to communicate directly with a high performing imaging card (Matrox Solios eA/XA), thus retaining high speed acquisition. The program is also designed to communicate with LabVIEW and Fiji for instrument control and image processing. Pscan 1.0 can handle high imaging rates and contains sufficient flexibility for users to adapt to their high speed imaging systems.

  1. Deciphering complex, functional structures with synchrotron-based absorption and phase contrast tomographic microscopy

    Science.gov (United States)

    Stampanoni, M.; Reichold, J.; Weber, B.; Haberthür, D.; Schittny, J.; Eller, J.; Büchi, F. N.; Marone, F.

    2010-09-01

    Nowadays, thanks to the high brilliance available at modern, third generation synchrotron facilities and recent developments in detector technology, it is possible to record volumetric information at the micrometer scale within few minutes. High signal-to-noise ratio, quantitative information on very complex structures like the brain micro vessel architecture, lung airways or fuel cells can be obtained thanks to the combination of dedicated sample preparation protocols, in-situ acquisition schemes and cutting-edge imaging analysis instruments. In this work we report on recent experiments carried out at the TOMCAT beamline of the Swiss Light Source [1] where synchrotron-based tomographic microscopy has been successfully used to obtain fundamental information on preliminary models for cerebral fluid flow [2], to provide an accurate mesh for 3D finite-element simulation of the alveolar structure of the pulmonary acinus [3] and to investigate the complex functional mechanism of fuel cells [4]. Further, we introduce preliminary results on the combination of absorption and phase contrast microscopy for the visualization of high-Z nanoparticles in soft tissues, a fundamental information when designing modern drug delivery systems [5]. As an outlook we briefly discuss the new possibilities offered by high sensitivity, high resolution grating interferomtery as well as Zernike Phase contrast nanotomography [6].

  2. All-optical optoacoustic microscopy based on probe beam deflection technique

    Directory of Open Access Journals (Sweden)

    Saher M. Maswadi

    2016-09-01

    Full Text Available Optoacoustic (OA microscopy using an all-optical system based on the probe beam deflection technique (PBDT for detection of laser-induced acoustic signals was investigated as an alternative to conventional piezoelectric transducers. PBDT provides a number of advantages for OA microscopy including (i efficient coupling of laser excitation energy to the samples being imaged through the probing laser beam, (ii undistorted coupling of acoustic waves to the detector without the need for separation of the optical and acoustic paths, (iii high sensitivity and (iv ultrawide bandwidth. Because of the unimpeded optical path in PBDT, diffraction-limited lateral resolution can be readily achieved. The sensitivity of the current PBDT sensor of 22 μV/Pa and its noise equivalent pressure (NEP of 11.4 Pa are comparable with these parameters of the optical micro-ring resonator and commercial piezoelectric ultrasonic transducers. Benefits of the present prototype OA microscope were demonstrated by successfully resolving micron-size details in histological sections of cardiac muscle.

  3. All-optical optoacoustic microscopy based on probe beam deflection technique.

    Science.gov (United States)

    Maswadi, Saher M; Ibey, Bennett L; Roth, Caleb C; Tsyboulski, Dmitri A; Beier, Hope T; Glickman, Randolph D; Oraevsky, Alexander A

    2016-09-01

    Optoacoustic (OA) microscopy using an all-optical system based on the probe beam deflection technique (PBDT) for detection of laser-induced acoustic signals was investigated as an alternative to conventional piezoelectric transducers. PBDT provides a number of advantages for OA microscopy including (i) efficient coupling of laser excitation energy to the samples being imaged through the probing laser beam, (ii) undistorted coupling of acoustic waves to the detector without the need for separation of the optical and acoustic paths, (iii) high sensitivity and (iv) ultrawide bandwidth. Because of the unimpeded optical path in PBDT, diffraction-limited lateral resolution can be readily achieved. The sensitivity of the current PBDT sensor of 22 μV/Pa and its noise equivalent pressure (NEP) of 11.4 Pa are comparable with these parameters of the optical micro-ring resonator and commercial piezoelectric ultrasonic transducers. Benefits of the present prototype OA microscope were demonstrated by successfully resolving micron-size details in histological sections of cardiac muscle.

  4. [Comparison of cell elasticity analysis methods based on atomic force microscopy indentation].

    Science.gov (United States)

    Wang, Zhe; Hao, Fengtao; Chen, Xiaohu; Yang, Zhouqi; Ding, Chong; Shang, Peng

    2014-10-01

    In order to investigate in greater detail the two methods based on Hertz model for analyzing force-distance curve obtained by atomic force microscopy, we acquired the force-distance curves of Hela and MCF-7 cells by atomic force microscopy (AFM) indentation in this study. After the determination of contact point, Young's modulus in different indentation depth were calculated with two analysis methods of "two point" and "slope fitting". The results showed that the Young's modulus of Hela cell was higher than that of MCF-7 cell,which is in accordance with the F-actin distribution of the two types of cell. We found that the Young's modulus of the cells was decreased with increasing indentation depth and the curve trends by "slope fitting". This indicated that the "slope fitting" method could reduce the error caused by the miscalculation of contact point. The purpose of this study was to provide a guidance for researcher to choose an appropriate method for analyzing AFM indentation force-distance curve.

  5. Transmission electron microscopy of InP-based compound semiconductor materials and devices

    International Nuclear Information System (INIS)

    Chu, S.N.G.

    1990-01-01

    InP/InGaAsP-based heteroepitaxial structures constitute the major optoelectronic devices for state-of-the-art long wavelength optical fiber communication system.s Future advanced device structures will require thin heteroepitaxial quantum wells and superlattices a few tens of angstrom or less in thickness, and lateral dimensions ranging from a few tens angstrom for quantum dots and wires to a few μm in width for buried heterostructure lasers. Due to the increasing complexity of the device structure required by band-gap engineering, the performance of these devices becomes susceptible to any lattice imperfections present in the structure. Transmission electron microscopy (TEM), therefore, becomes the most important technique in characterizing the structural integrity of these materials. Cross-section transmission electron microscopy (XTEM) not only provides the necessary geometric information on the device structure; a careful study of the materials science behind the observed lattice imperfections provides directions for optimization of both the epitaxial growth parameters and device processing conditions. Furthermore, for device reliability studies, TEM is the only technique that unambiguously identifies the cause of device degradation. In this paper, the authors discuss areas of application of various TEM techniques, describe the TEM sample preparation technique, and review case studies to demonstrate the power of the TEM technique

  6. Adjustable liquid aperture to eliminate undesirable light in holographic projection.

    Science.gov (United States)

    Wang, Di; Liu, Chao; Li, Lei; Zhou, Xin; Wang, Qiong-Hua

    2016-02-08

    In this paper, we propose an adjustable liquid aperture to eliminate the undesirable light in a holographic projection. The aperture is based on hydrodynamic actuation. A chamber is formed with a cylindrical tube. A black droplet is filled in the sidewall of the cylinder tube and the outside space is the transparent oil which is immiscible with the black droplet. An ultrathin glass sheet is attached on the bottom substrate of the device and a black shading film is secured to the central area of the glass sheet. By changing the volume of the black droplet, the black droplet will move to the middle or sidewall due to hydrodynamic actuation, so the device can be used as an adjustable aperture. A divergent spherical wave and a solid lens are used to separate the focus planes of the reconstructed image and diffraction beams induced by the liquid crystal on silicon in the holographic projection. Then the aperture is used to eliminate the diffraction beams by adjusting the size of the liquid aperture and the holographic projection does not have undesirable light.

  7. Deuteron transverse densities in holographic QCD

    Energy Technology Data Exchange (ETDEWEB)

    Mondal, Chandan [Chinese Academy of Sciences, Institute of Modern Physics, Lanzhou (China); Indian Institute of Technology Kanpur, Department of Physics, Kanpur (India); Chakrabarti, Dipankar [Indian Institute of Technology Kanpur, Department of Physics, Kanpur (India); Zhao, Xingbo [Chinese Academy of Sciences, Institute of Modern Physics, Lanzhou (China)

    2017-05-15

    We investigate the transverse charge density in the longitudinally as well as transversely polarized deuteron using the recent empirical description of the deuteron electromagnetic form factors in the framework of holographic QCD. The predictions of the holographic QCD are compared with the results of a standard phenomenological parameterization. In addition, we evaluate GPDs and the gravitational form factors for the deuteron. The longitudinal momentum densities are also investigated in the transverse plane. (orig.)

  8. Proton beam writing for producing holographic images

    International Nuclear Information System (INIS)

    Ow, Y.S.; Breese, M.B.H.; Bettiol, A.A.

    2009-01-01

    This work reports on the writing of computer generated hologram diffraction patterns using focused 2 MeV proton beam irradiation. These patterns were designed using a ray tracing algorithm and written directly into a thick polymethylmethacrylate layer. When the developed holographic pattern was illuminated with a 650 nm laser it produced a good reconstructed image. This work provides means of forming high-resolution, high aspect ratio holographic images in polymers for applications in data storage using switchable holography.

  9. The Holographic Weyl anomaly

    CERN Document Server

    Henningson, M; Henningson, Mans; Skenderis, Kostas

    1998-01-01

    We calculate the Weyl anomaly for conformal field theories that can be described via the adS/CFT correspondence. This entails regularizing the gravitational part of the corresponding supergravity action in a manner consistent with general covariance. Up to a constant, the anomaly only depends on the dimension d of the manifold on which the conformal field theory is defined. We present concrete expressions for the anomaly in the physically relevant cases d = 2, 4 and 6. In d = 2 we find for the central charge c = 3 l/ 2 G_N in agreement with considerations based on the asymptotic symmetry algebra of adS_3. In d = 4 the anomaly agrees precisely with that of the corresponding N = 4 superconformal SU(N) gauge theory. The result in d = 6 provides new information for the (0, 2) theory, since its Weyl anomaly has not been computed previously. The anomaly in this case grows as N^3, where N is the number of coincident M5 branes, and it vanishes for a Ricci-flat background.

  10. Context based mixture model for cell phase identification in automated fluorescence microscopy

    Directory of Open Access Journals (Sweden)

    Zhou Xiaobo

    2007-01-01

    Full Text Available Abstract Background Automated identification of cell cycle phases of individual live cells in a large population captured via automated fluorescence microscopy technique is important for cancer drug discovery and cell cycle studies. Time-lapse fluorescence microscopy images provide an important method to study the cell cycle process under different conditions of perturbation. Existing methods are limited in dealing with such time-lapse data sets while manual analysis is not feasible. This paper presents statistical data analysis and statistical pattern recognition to perform this task. Results The data is generated from Hela H2B GFP cells imaged during a 2-day period with images acquired 15 minutes apart using an automated time-lapse fluorescence microscopy. The patterns are described with four kinds of features, including twelve general features, Haralick texture features, Zernike moment features, and wavelet features. To generate a new set of features with more discriminate power, the commonly used feature reduction techniques are used, which include Principle Component Analysis (PCA, Linear Discriminant Analysis (LDA, Maximum Margin Criterion (MMC, Stepwise Discriminate Analysis based Feature Selection (SDAFS, and Genetic Algorithm based Feature Selection (GAFS. Then, we propose a Context Based Mixture Model (CBMM for dealing with the time-series cell sequence information and compare it to other traditional classifiers: Support Vector Machine (SVM, Neural Network (NN, and K-Nearest Neighbor (KNN. Being a standard practice in machine learning, we systematically compare the performance of a number of common feature reduction techniques and classifiers to select an optimal combination of a feature reduction technique and a classifier. A cellular database containing 100 manually labelled subsequence is built for evaluating the performance of the classifiers. The generalization error is estimated using the cross validation technique. The

  11. Image plane digital holographic microscope for the inspection of ferroelectric single crystals.

    Czech Academy of Sciences Publication Activity Database

    Psota, Pavel; Mokrý, Pavel; Lédl, Vít; Vojtíšek, Petr

    2016-01-01

    Roč. 55, č. 12 (2016), č. článku 121731. ISSN 0091-3286 R&D Projects: GA ČR(CZ) GA14-32228S Institutional support: RVO:61389021 Keywords : Digital holography * barium titanate * domain pattern * ferroelectric crystals * holographic microscopy Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering Impact factor: 1.082, year: 2016 http://dx.doi.org/10.1117/1.OE.55.12.121731

  12. Soft Pomeron in Holographic QCD

    CERN Document Server

    Ballon-Bayona, Alfonso; Costa, Miguel S; Djurić, Marko

    2016-01-01

    We study the graviton Regge trajectory in Holographic QCD as a model for high energy scattering processes dominated by soft pomeron exchange. This is done by considering spin J fields from the closed string sector that are dual to glueball states of even spin and parity. In particular, we construct a model that governs the analytic continuation of the spin J field equation to the region of real J < 2, which includes the scattering domain of negative Maldelstam variable t. The model leads to approximately linear Regge trajectories and is compatible with the measured values of 1.08 for the intercept and 0.25 GeV$^{-2}$ for the slope of the soft pomeron. The intercept of the secondary pomeron trajectory is in the same region of the subleading trajectories, made of mesons, proposed by Donnachie and Landshoff, and should therefore be taken into account.

  13. Defect CFTs and holographic multiverse

    Energy Technology Data Exchange (ETDEWEB)

    Fiol, Bartomeu, E-mail: bfiol@ub.edu [Departament de Física Fonamental i Institut de Ciències del Cosmos, Universitat de Barcelona, Martí i Franquès 1, 08193 Barcelona (Spain)

    2010-07-01

    We investigate some aspects of a recent proposal for a holographic description of the multiverse. Specifically, we focus on the implications on the suggested duality of the fluctuations of a bubble separating two universes with different cosmological constants. We do so by considering a similar problem in a 2+1 CFT with a codimension one defect, obtained by an M5-brane probe embedding in AdS{sub 4} × S{sup 7}, and studying its spectrum of fluctuations. Our results suggest that the kind of behavior required by the spectrum of bubble fluctuations is not likely to take place in defect CFTs with an AdS dual, although it might be possible if the defect supports a non-unitary theory.

  14. Defect CFTs and holographic multiverse

    International Nuclear Information System (INIS)

    Fiol, Bartomeu

    2010-01-01

    We investigate some aspects of a recent proposal for a holographic description of the multiverse. Specifically, we focus on the implications on the suggested duality of the fluctuations of a bubble separating two universes with different cosmological constants. We do so by considering a similar problem in a 2+1 CFT with a codimension one defect, obtained by an M5-brane probe embedding in AdS 4 × S 7 , and studying its spectrum of fluctuations. Our results suggest that the kind of behavior required by the spectrum of bubble fluctuations is not likely to take place in defect CFTs with an AdS dual, although it might be possible if the defect supports a non-unitary theory

  15. Emergent Spacetime and Holographic CFTs

    CERN Document Server

    El-Showk, Sheer

    2012-01-01

    We discuss universal properties of conformal field theories with holographic duals. A central feature of these theories is the existence of a low-lying sector of operators whose correlators factorize. We demonstrate that factorization can only hold in the large central charge limit. Using conformal invariance and factorization we argue that these operators are naturally represented as fields in AdS as this makes the underlying linearity of the system manifest. In this class of CFTs the solution of the conformal bootstrap conditions can be naturally organized in structures which coincide with Witten diagrams in the bulk. The large value of the central charge suggests that the theory must include a large number of new operators not captured by the factorized sector. Consequently we may think of the AdS hologram as an effective representation of a small sector of the CFT, which is embedded inside a much larger Hilbert space corresponding to the black hole microstates.

  16. Survey of holographic security systems

    Science.gov (United States)

    Kontnik, Lewis T.; Lancaster, Ian M.

    1990-04-01

    The counterfeiting of products and financial instruments is a major problem throughout the world today. The dimensions of the problem are growing, accelerated by the expanding availability of production technologies to sophisticated counterfeiters and the increasing capabilities of these technologies. Various optical techniques, including holography, are beingused in efforts to mark authentic products and to distinguish them from copies. Industry is recognizing that the effectiveness of these techniques depends on such factors as the economics of the counterfeiting process and the distribution channels for the products involved, in addition to the performance of the particular optical security technologies used. This paper surveys the nature of the growing counterfeit market place and reviews the utility of holographic optical security systems. In particular, we review the use of holograms on credit cards and other products; and outline certain steps the holography industry should take to promote these application.

  17. Enhanced depth-of-field of an integral imaging microscope using a bifocal holographic optical element-micro lens array.

    Science.gov (United States)

    Kwon, Ki-Chul; Lim, Young-Tae; Shin, Chang-Won; Erdenebat, Munkh-Uchral; Hwang, Jae-Moon; Kim, Nam

    2017-08-15

    We propose and implement an integral imaging microscope with extended depth-of-field (DoF) using a bifocal holographic micro lens array (MLA). The properties of the two MLAs are switched via peristrophic multiplexing, where different properties of the MLA are recorded onto the single holographic optical element (HOE). The recorded MLA properties are perpendicular to each other: after the first mode is recorded, the HOE is rotated by 90° clockwise, and the second mode is recorded. The experimental results confirm that the DoF of the integral imaging microscopy system is extended successfully by using the bifocal MLA.

  18. Characterizing the surface forces between two individual nanowires using optical microscopy based nanomanipulation

    Science.gov (United States)

    Xie, Hongtao; Mead, James L.; Wang, Shiliang; Fatikow, Sergej; Huang, Han

    2018-06-01

    The adhesion and friction between two Al2O3 nanowires (NWs) was characterized by the use of optical microscopy based nanomanipulation, with which peeling, shearing and sliding was performed. The elastically deformed shape of the NWs during peeling and shearing was used to calculate the adhesion and frictional forces; force sensing was not required. The obtained adhesion stress between two Al2O3 NWs varied from 0.14 to 0.25 MPa, lower than that observed for carbon nanotube junctions, and was attributed to van der Waals attraction. Stick-slip was observed during the shearing and sliding of two NWs, and was the consequence of discrete contact between surface asperities. The obtained static and kinetic frictional stresses varied from 0.7 to 1.3 MPa and 0.4 to 0.8 MPa, respectively; significantly greater than the obtained adhesion stress.

  19. Simulation-based Extraction of Key Material Parameters from Atomic Force Microscopy

    Science.gov (United States)

    Alsafi, Huseen; Peninngton, Gray

    Models for the atomic force microscopy (AFM) tip and sample interaction contain numerous material parameters that are often poorly known. This is especially true when dealing with novel material systems or when imaging samples that are exposed to complicated interactions with the local environment. In this work we use Monte Carlo methods to extract sample material parameters from the experimental AFM analysis of a test sample. The parameterized theoretical model that we use is based on the Virtual Environment for Dynamic AFM (VEDA) [1]. The extracted material parameters are then compared with the accepted values for our test sample. Using this procedure, we suggest a method that can be used to successfully determine unknown material properties in novel and complicated material systems. We acknowledge Fisher Endowment Grant support from the Jess and Mildred Fisher College of Science and Mathematics,Towson University.

  20. Structural and dynamical aspects of skin studied by multiphoton excitation fluorescence microscopy-based methods

    DEFF Research Database (Denmark)

    Bloksgaard, Maria; Brewer, Jonathan R.; Bagatolli, Luis

    2013-01-01

    ' parameters. Specifically, by applying these methods, spatially resolved maps of water dipolar relaxation (generalized polarization function using the 6-lauroyl-2-(N,N-dimethylamino)naphthale probe), activity of protons (fluorescence lifetime imaging using a proton sensitive fluorescence probe--2,7-bis-(2......-carboxyethyl)-5-(and-6)-carboxyfluorescein) and diffusion coefficients of distinct fluorescence probes (raster imaging correlation spectroscopy) can be obtained from different regions of the tissue. Comparative studies of different tissue strata, but also between equivalent regions of normal and abnormal......This mini-review reports on applications of particular multiphoton excitation microscopy-based methodologies employed in our laboratory to study skin. These approaches allow in-depth optical sectioning of the tissue, providing spatially resolved information on specific fluorescence probes...

  1. Imaging a Large Sample with Selective Plane Illumination Microscopy Based on Multiple Fluorescent Microsphere Tracking

    Science.gov (United States)

    Ryu, Inkeon; Kim, Daekeun

    2018-04-01

    A typical selective plane illumination microscopy (SPIM) image size is basically limited by the field of view, which is a characteristic of the objective lens. If an image larger than the imaging area of the sample is to be obtained, image stitching, which combines step-scanned images into a single panoramic image, is required. However, accurately registering the step-scanned images is very difficult because the SPIM system uses a customized sample mount where uncertainties for the translational and the rotational motions exist. In this paper, an image registration technique based on multiple fluorescent microsphere tracking is proposed in the view of quantifying the constellations and measuring the distances between at least two fluorescent microspheres embedded in the sample. Image stitching results are demonstrated for optically cleared large tissue with various staining methods. Compensation for the effect of the sample rotation that occurs during the translational motion in the sample mount is also discussed.

  2. [In Vivo Study of Chitin in Fungal Hyphae Based on Confocal Raman Microscopy].

    Science.gov (United States)

    Li, Xiao-li; Luo, Liu-bin; Zhou, Bin-xiong; Hu, Xiao-qian; Sun, Chan-jun; He, Yong

    2016-01-01

    Chitin is an important structural polysaccharide of fungal cell wall. In this paper, aerial hyphae of Colletotrichum camelliae Massee was first studied by confocal Raman microscopy in vivo. Firstly, the optimal experimental parameters of hyphae for collecting the Raman spectra were determined, and the typical Raman spectra of hyphae, chitin standard and background were acquired. By comparing analysis, characteristic peaks of chitin were found in hyphae. Then, a region of interesting on hyphae was selected for Raman scanning. Through principal component analysis, the Raman signal of hyphae and background in the scanning area can be separated clearly. Combined with loading weight plot, two main characteristic peaks of hyphae were obtained, 1 622 cm(-1) was belong to chitin and 1 368 cm(-1) was assigned to pectic polysaccharide. Finally, two and three dimension chemical images of fungal hyphae were realized based on Raman fingerprint spectra of chitin in a nondestructive way.

  3. Passive microrheology of soft materials with atomic force microscopy: A wavelet-based spectral analysis

    Energy Technology Data Exchange (ETDEWEB)

    Martinez-Torres, C.; Streppa, L. [CNRS, UMR5672, Laboratoire de Physique, Ecole Normale Supérieure de Lyon, 46 Allée d' Italie, Université de Lyon, 69007 Lyon (France); Arneodo, A.; Argoul, F. [CNRS, UMR5672, Laboratoire de Physique, Ecole Normale Supérieure de Lyon, 46 Allée d' Italie, Université de Lyon, 69007 Lyon (France); CNRS, UMR5798, Laboratoire Ondes et Matière d' Aquitaine, Université de Bordeaux, 351 Cours de la Libération, 33405 Talence (France); Argoul, P. [Université Paris-Est, Ecole des Ponts ParisTech, SDOA, MAST, IFSTTAR, 14-20 Bd Newton, Cité Descartes, 77420 Champs sur Marne (France)

    2016-01-18

    Compared to active microrheology where a known force or modulation is periodically imposed to a soft material, passive microrheology relies on the spectral analysis of the spontaneous motion of tracers inherent or external to the material. Passive microrheology studies of soft or living materials with atomic force microscopy (AFM) cantilever tips are rather rare because, in the spectral densities, the rheological response of the materials is hardly distinguishable from other sources of random or periodic perturbations. To circumvent this difficulty, we propose here a wavelet-based decomposition of AFM cantilever tip fluctuations and we show that when applying this multi-scale method to soft polymer layers and to living myoblasts, the structural damping exponents of these soft materials can be retrieved.

  4. Compact three-dimensional super-resolution system based on fluorescence emission difference microscopy

    Science.gov (United States)

    Zhu, Dazhao; Chen, Youhua; Fang, Yue; Hussain, Anwar; Kuang, Cuifang; Zhou, Xiaoxu; Xu, Yingke; Liu, Xu

    2017-12-01

    A compact microscope system for three-dimensional (3-D) super-resolution imaging is presented. The super-resolution capability of the system is based on a size-reduced effective 3-D point spread function generated through the fluorescence emission difference (FED) method. The appropriate polarization direction distribution and manipulation allows the panel active area of the spatial light modulator to be fully utilized. This allows simultaneous modulation of the incident light by two kinds of phase masks to be performed with a single spatial light modulator in order to generate a 3-D negative spot. The system is more compact than standard 3-D FED systems while maintaining all the advantages of 3-D FED microscopy. The experimental results demonstrated the improvement in 3-D resolution by nearly 1.7 times and 1.6 times compared to the classic confocal resolution in the lateral and axial directions, respectively.

  5. Opto-fluidics based microscopy and flow cytometry on a cell phone for blood analysis.

    Science.gov (United States)

    Zhu, Hongying; Ozcan, Aydogan

    2015-01-01

    Blood analysis is one of the most important clinical tests for medical diagnosis. Flow cytometry and optical microscopy are widely used techniques to perform blood analysis and therefore cost-effective translation of these technologies to resource limited settings is critical for various global health as well as telemedicine applications. In this chapter, we review our recent progress on the integration of imaging flow cytometry and fluorescent microscopy on a cell phone using compact, light-weight and cost-effective opto-fluidic attachments integrated onto the camera module of a smartphone. In our cell-phone based opto-fluidic imaging cytometry design, fluorescently labeled cells are delivered into the imaging area using a disposable micro-fluidic chip that is positioned above the existing camera unit of the cell phone. Battery powered light-emitting diodes (LEDs) are butt-coupled to the sides of this micro-fluidic chip without any lenses, which effectively acts as a multimode slab waveguide, where the excitation light is guided to excite the fluorescent targets within the micro-fluidic chip. Since the excitation light propagates perpendicular to the detection path, an inexpensive plastic absorption filter is able to reject most of the scattered light and create a decent dark-field background for fluorescent imaging. With this excitation geometry, the cell-phone camera can record fluorescent movies of the particles/cells as they are flowing through the microchannel. The digital frames of these fluorescent movies are then rapidly processed to quantify the count and the density of the labeled particles/cells within the solution under test. With a similar opto-fluidic design, we have recently demonstrated imaging and automated counting of stationary blood cells (e.g., labeled white blood cells or unlabeled red blood cells) loaded within a disposable cell counting chamber. We tested the performance of this cell-phone based imaging cytometry and blood analysis platform

  6. Image-based overlay measurement using subsurface ultrasonic resonance force microscopy

    Science.gov (United States)

    Tamer, M. S.; van der Lans, M. J.; Sadeghian, H.

    2018-03-01

    Image Based Overlay (IBO) measurement is one of the most common techniques used in Integrated Circuit (IC) manufacturing to extract the overlay error values. The overlay error is measured using dedicated overlay targets which are optimized to increase the accuracy and the resolution, but these features are much larger than the IC feature size. IBO measurements are realized on the dedicated targets instead of product features, because the current overlay metrology solutions, mainly based on optics, cannot provide sufficient resolution on product features. However, considering the fact that the overlay error tolerance is approaching 2 nm, the overlay error measurement on product features becomes a need for the industry. For sub-nanometer resolution metrology, Scanning Probe Microscopy (SPM) is widely used, though at the cost of very low throughput. The semiconductor industry is interested in non-destructive imaging of buried structures under one or more layers for the application of overlay and wafer alignment, specifically through optically opaque media. Recently an SPM technique has been developed for imaging subsurface features which can be potentially considered as a solution for overlay metrology. In this paper we present the use of Subsurface Ultrasonic Resonance Force Microscopy (SSURFM) used for IBO measurement. We used SSURFM for imaging the most commonly used overlay targets on a silicon substrate and photoresist. As a proof of concept we have imaged surface and subsurface structures simultaneously. The surface and subsurface features of the overlay targets are fabricated with programmed overlay errors of +/-40 nm, +/-20 nm, and 0 nm. The top layer thickness changes between 30 nm and 80 nm. Using SSURFM the surface and subsurface features were successfully imaged and the overlay errors were extracted, via a rudimentary image processing algorithm. The measurement results are in agreement with the nominal values of the programmed overlay errors.

  7. Wide field of view common-path lateral-shearing digital holographic interference microscope.

    Science.gov (United States)

    Vora, Priyanka; Trivedi, Vismay; Mahajan, Swapnil; Patel, Nimit; Joglekar, Mugdha; Chhaniwal, Vani; Moradi, Ali-Reza; Javidi, Bahram; Anand, Arun

    2017-12-01

    Quantitative three-dimensional (3-D) imaging of living cells provides important information about the cell morphology and its time variation. Off-axis, digital holographic interference microscopy is an ideal tool for 3-D imaging, parameter extraction, and classification of living cells. Two-beam digital holographic microscopes, which are usually employed, provide high-quality 3-D images of micro-objects, albeit with lower temporal stability. Common-path digital holographic geometries, in which the reference beam is derived from the object beam, provide higher temporal stability along with high-quality 3-D images. Self-referencing geometry is the simplest of the common-path techniques, in which a portion of the object beam itself acts as the reference, leading to compact setups using fewer optical elements. However, it has reduced field of view, and the reference may contain object information. Here, we describe the development of a common-path digital holographic microscope, employing a shearing plate and converting one of the beams into a separate reference by employing a pin-hole. The setup is as compact as self-referencing geometry, while providing field of view as wide as that of a two-beam microscope. The microscope is tested by imaging and quantifying the morphology and dynamics of human erythrocytes. (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).

  8. Novel pH-sensitive photopolymer hydrogel and its holographic sensing response for solution characterization

    Science.gov (United States)

    Liu, Hongpeng; Yu, Dan; Zhou, Ke; Wang, Shichan; Luo, Suhua; Li, Li; Wang, Weibo; Song, Qinggong

    2018-05-01

    Optical sensor based on pH-sensitive hydrogel has important practical applications in medical diagnosis and bio-sensor areas. This report details the experimental and theoretical results from a novel photosensitive polymer hydrogel holographic sensor, which formed by thermal polymerization of 2-hydroxyethyl methacrylate, for the detection of pH in buffer. Volume grating recorded in the polymer hydrogel was employed in response to the performance of solution. Methacrylic acid with carboxyl groups was selected as the primary co-monomer to functionalize the matrix. Peak diffraction spectrum of holographic grating determined as a primary sensing parameter was characterized to reflect the change in pH. The extracted linear relation between peak wavelength and pH value provided a probability for the practical application of holographic sensor. To explore the sensing mechanism deeply, a theoretical model was used to describe the relevant holographic processes, including grating formation, dark diffusional enhancement, and final fringe swelling. Numerical result further showed all of the dynamic processes and internal sensing physical mechanism. These experimental and numerical results provided a significant foundation for the development of novel holographic sensor based on polymer hydrogel and improvement of its practical applicability.

  9. Quantum­holographic framework for psychosomatics and spirituality: Complete healing and spiritual integration without a mask

    OpenAIRE

    Raković, Dejan

    2017-01-01

    The subject of this paper is quantum­holographic framework for holistic psychosomatics (including integrative medicine and transpersonal psychology). Such a framework could have significant implications for understanding the mechanisms of quantum­holographic feedback control in the morphogenesis and bio­resonant application of the healing boundary conditions in psychosomatics, based on acupuncture and consciousness. It sheds new light on the long standing open problems of the holistic role an...

  10. Three-directional motion-compensation mask-based novel look-up table on graphics processing units for video-rate generation of digital holographic videos of three-dimensional scenes.

    Science.gov (United States)

    Kwon, Min-Woo; Kim, Seung-Cheol; Kim, Eun-Soo

    2016-01-20

    A three-directional motion-compensation mask-based novel look-up table method is proposed and implemented on graphics processing units (GPUs) for video-rate generation of digital holographic videos of three-dimensional (3D) scenes. Since the proposed method is designed to be well matched with the software and memory structures of GPUs, the number of compute-unified-device-architecture kernel function calls can be significantly reduced. This results in a great increase of the computational speed of the proposed method, allowing video-rate generation of the computer-generated hologram (CGH) patterns of 3D scenes. Experimental results reveal that the proposed method can generate 39.8 frames of Fresnel CGH patterns with 1920×1080 pixels per second for the test 3D video scenario with 12,088 object points on dual GPU boards of NVIDIA GTX TITANs, and they confirm the feasibility of the proposed method in the practical application fields of electroholographic 3D displays.

  11. High-throughput full-automatic synchrotron-based tomographic microscopy

    International Nuclear Information System (INIS)

    Mader, Kevin; Marone, Federica; Hintermueller, Christoph; Mikuljan, Gordan; Isenegger, Andreas; Stampanoni, Marco

    2011-01-01

    At the TOMCAT (TOmographic Microscopy and Coherent rAdiology experimenTs) beamline of the Swiss Light Source with an energy range of 8-45 keV and voxel size from 0.37 (micro)m to 7.4 (micro)m, full tomographic datasets are typically acquired in 5 to 10 min. To exploit the speed of the system and enable high-throughput studies to be performed in a fully automatic manner, a package of automation tools has been developed. The samples are automatically exchanged, aligned, moved to the correct region of interest, and scanned. This task is accomplished through the coordination of Python scripts, a robot-based sample-exchange system, sample positioning motors and a CCD camera. The tools are suited for any samples that can be mounted on a standard SEM stub, and require no specific environmental conditions. Up to 60 samples can be analyzed at a time without user intervention. The throughput of the system is dependent on resolution, energy and sample size, but rates of four samples per hour have been achieved with 0.74 (micro)m voxel size at 17.5 keV. The maximum intervention-free scanning time is theoretically unlimited, and in practice experiments have been running unattended as long as 53 h (the average beam time allocation at TOMCAT is 48 h per user). The system is the first fully automated high-throughput tomography station: mounting samples, finding regions of interest, scanning and reconstructing can be performed without user intervention. The system also includes many features which accelerate and simplify the process of tomographic microscopy.

  12. The influence of physical and physiological cues on atomic force microscopy-based cell stiffness assessment.

    Directory of Open Access Journals (Sweden)

    Yu-Wei Chiou

    Full Text Available Atomic force microscopy provides a novel technique for differentiating the mechanical properties of various cell types. Cell elasticity is abundantly used to represent the structural strength of cells in different conditions. In this study, we are interested in whether physical or physiological cues affect cell elasticity in Atomic force microscopy (AFM-based assessments. The physical cues include the geometry of the AFM tips, the indenting force and the operating temperature of the AFM. All of these cues show a significant influence on the cell elasticity assessment. Sharp AFM tips create a two-fold increase in the value of the effective Young's modulus (E(eff relative to that of the blunt tips. Higher indenting force at the same loading rate generates higher estimated cell elasticity. Increasing the operation temperature of the AFM leads to decreases in the cell stiffness because the structure of actin filaments becomes disorganized. The physiological cues include the presence of fetal bovine serum or extracellular matrix-coated surfaces, the culture passage number, and the culture density. Both fetal bovine serum and the extracellular matrix are critical for cells to maintain the integrity of actin filaments and consequently exhibit higher elasticity. Unlike primary cells, mouse kidney progenitor cells can be passaged and maintain their morphology and elasticity for a very long period without a senescence phenotype. Finally, cell elasticity increases with increasing culture density only in MDCK epithelial cells. In summary, for researchers who use AFM to assess cell elasticity, our results provide basic and significant information about the suitable selection of physical and physiological cues.

  13. The influence of physical and physiological cues on atomic force microscopy-based cell stiffness assessment.

    Science.gov (United States)

    Chiou, Yu-Wei; Lin, Hsiu-Kuan; Tang, Ming-Jer; Lin, Hsi-Hui; Yeh, Ming-Long

    2013-01-01

    Atomic force microscopy provides a novel technique for differentiating the mechanical properties of various cell types. Cell elasticity is abundantly used to represent the structural strength of cells in different conditions. In this study, we are interested in whether physical or physiological cues affect cell elasticity in Atomic force microscopy (AFM)-based assessments. The physical cues include the geometry of the AFM tips, the indenting force and the operating temperature of the AFM. All of these cues show a significant influence on the cell elasticity assessment. Sharp AFM tips create a two-fold increase in the value of the effective Young's modulus (E(eff)) relative to that of the blunt tips. Higher indenting force at the same loading rate generates higher estimated cell elasticity. Increasing the operation temperature of the AFM leads to decreases in the cell stiffness because the structure of actin filaments becomes disorganized. The physiological cues include the presence of fetal bovine serum or extracellular matrix-coated surfaces, the culture passage number, and the culture density. Both fetal bovine serum and the extracellular matrix are critical for cells to maintain the integrity of actin filaments and consequently exhibit higher elasticity. Unlike primary cells, mouse kidney progenitor cells can be passaged and maintain their morphology and elasticity for a very long period without a senescence phenotype. Finally, cell elasticity increases with increasing culture density only in MDCK epithelial cells. In summary, for researchers who use AFM to assess cell elasticity, our results provide basic and significant information about the suitable selection of physical and physiological cues.

  14. Spline-based image-to-volume registration for three-dimensional electron microscopy

    International Nuclear Information System (INIS)

    Jonic, S.; Sorzano, C.O.S.; Thevenaz, P.; El-Bez, C.; De Carlo, S.; Unser, M.

    2005-01-01

    This paper presents an algorithm based on a continuous framework for a posteriori angular and translational assignment in three-dimensional electron microscopy (3DEM) of single particles. Our algorithm can be used advantageously to refine the assignment of standard quantized-parameter methods by registering the images to a reference 3D particle model. We achieve the registration by employing a gradient-based iterative minimization of a least-squares measure of dissimilarity between an image and a projection of the volume in the Fourier transform (FT) domain. We compute the FT of the projection using the central-slice theorem (CST). To compute the gradient accurately, we take advantage of a cubic B-spline model of the data in the frequency domain. To improve the robustness of the algorithm, we weight the cost function in the FT domain and apply a 'mixed' strategy for the assignment based on the minimum value of the cost function at registration for several different initializations. We validate our algorithm in a fully controlled simulation environment. We show that the mixed strategy improves the assignment accuracy; on our data, the quality of the angular and translational assignment was better than 2 voxel (i.e., 6.54 A). We also test the performance of our algorithm on real EM data. We conclude that our algorithm outperforms a standard projection-matching refinement in terms of both consistency of 3D reconstructions and speed

  15. Comparing holographic dark energy models with statefinder

    International Nuclear Information System (INIS)

    Cui, Jing-Lei; Zhang, Jing-Fei

    2014-01-01

    We apply the statefinder diagnostic to the holographic dark energy models, including the original holographic dark energy (HDE) model, the new holographic dark energy model, the new agegraphic dark energy (NADE) model, and the Ricci dark energy model. In the low-redshift region the holographic dark energy models are degenerate with each other and with the ΛCDM model in the H(z) and q(z) evolutions. In particular, the HDE model is highly degenerate with the ΛCDM model, and in the HDE model the cases with different parameter values are also in strong degeneracy. Since the observational data are mainly within the low-redshift region, it is very important to break this lowredshift degeneracy in the H(z) and q(z) diagnostics by using some quantities with higher order derivatives of the scale factor. It is shown that the statefinder diagnostic r(z) is very useful in breaking the low-redshift degeneracies. By employing the statefinder diagnostic the holographic dark energy models can be differentiated efficiently in the low-redshift region. The degeneracy between the holographic dark energy models and the ΛCDM model can also be broken by this method. Especially for the HDE model, all the previous strong degeneracies appearing in the H(z) and q(z) diagnostics are broken effectively. But for the NADE model, the degeneracy between the cases with different parameter values cannot be broken, even though the statefinder diagnostic is used. A direct comparison of the holographic dark energy models in the r-s plane is also made, in which the separations between the models (including the ΛCDM model) can be directly measured in the light of the current values {r 0 , s 0 } of the models. (orig.)

  16. Fourier transform digital holographic adaptive optics imaging system

    Science.gov (United States)

    Liu, Changgeng; Yu, Xiao; Kim, Myung K.

    2013-01-01

    A Fourier transform digital holographic adaptive optics imaging system and its basic principles are proposed. The CCD is put at the exact Fourier transform plane of the pupil of the eye lens. The spherical curvature introduced by the optics except the eye lens itself is eliminated. The CCD is also at image plane of the target. The point-spread function of the system is directly recorded, making it easier to determine the correct guide-star hologram. Also, the light signal will be stronger at the CCD, especially for phase-aberration sensing. Numerical propagation is avoided. The sensor aperture has nothing to do with the resolution and the possibility of using low coherence or incoherent illumination is opened. The system becomes more efficient and flexible. Although it is intended for ophthalmic use, it also shows potential application in microscopy. The robustness and feasibility of this compact system are demonstrated by simulations and experiments using scattering objects. PMID:23262541

  17. Statistical region based active contour using a fractional entropy descriptor: Application to nuclei cell segmentation in confocal \\ud microscopy images

    OpenAIRE

    Histace, A; Meziou, B J; Matuszewski, Bogdan; Precioso, F; Murphy, M F; Carreiras, F

    2013-01-01

    We propose an unsupervised statistical region based active contour approach integrating an original fractional entropy measure for image segmentation with a particular application to single channel actin tagged fluorescence confocal microscopy image segmentation. Following description of statistical based active contour segmentation and the mathematical definition of the proposed fractional entropy descriptor, we demonstrate comparative segmentation results between the proposed approach and s...

  18. Molecular dynamics-based refinement and validation for sub-5 Å cryo-electron microscopy maps.

    Science.gov (United States)

    Singharoy, Abhishek; Teo, Ivan; McGreevy, Ryan; Stone, John E; Zhao, Jianhua; Schulten, Klaus

    2016-07-07

    Two structure determination methods, based on the molecular dynamics flexible fitting (MDFF) paradigm, are presented that resolve sub-5 Å cryo-electron microscopy (EM) maps with either single structures or ensembles of such structures. The methods, denoted cascade MDFF and resolution exchange MDFF, sequentially re-refine a search model against a series of maps of progressively higher resolutions, which ends with the original experimental resolution. Application of sequential re-refinement enables MDFF to achieve a radius of convergence of ~25 Å demonstrated with the accurate modeling of β-galactosidase and TRPV1 proteins at 3.2 Å and 3.4 Å resolution, respectively. The MDFF refinements uniquely offer map-model validation and B-factor determination criteria based on the inherent dynamics of the macromolecules studied, captured by means of local root mean square fluctuations. The MDFF tools described are available to researchers through an easy-to-use and cost-effective cloud computing resource on Amazon Web Services.

  19. A wavelet-based Bayesian framework for 3D object segmentation in microscopy

    Science.gov (United States)

    Pan, Kangyu; Corrigan, David; Hillebrand, Jens; Ramaswami, Mani; Kokaram, Anil

    2012-03-01

    In confocal microscopy, target objects are labeled with fluorescent markers in the living specimen, and usually appear with irregular brightness in the observed images. Also, due to the existence of out-of-focus objects in the image, the segmentation of 3-D objects in the stack of image slices captured at different depth levels of the specimen is still heavily relied on manual analysis. In this paper, a novel Bayesian model is proposed for segmenting 3-D synaptic objects from given image stack. In order to solve the irregular brightness and out-offocus problems, the segmentation model employs a likelihood using the luminance-invariant 'wavelet features' of image objects in the dual-tree complex wavelet domain as well as a likelihood based on the vertical intensity profile of the image stack in 3-D. Furthermore, a smoothness 'frame' prior based on the a priori knowledge of the connections of the synapses is introduced to the model for enhancing the connectivity of the synapses. As a result, our model can successfully segment the in-focus target synaptic object from a 3D image stack with irregular brightness.

  20. A Dual Microscopy-Based Assay To Assess Listeria monocytogenes Cellular Entry and Vacuolar Escape.

    Science.gov (United States)

    Quereda, Juan J; Pizarro-Cerdá, Javier; Balestrino, Damien; Bobard, Alexandre; Danckaert, Anne; Aulner, Nathalie; Shorte, Spencer; Enninga, Jost; Cossart, Pascale

    2016-01-01

    Listeria monocytogenes is a Gram-positive bacterium and a facultative intracellular pathogen that invades mammalian cells, disrupts its internalization vacuole, and proliferates in the host cell cytoplasm. Here, we describe a novel image-based microscopy assay that allows discrimination between cellular entry and vacuolar escape, enabling high-content screening to identify factors specifically involved in these two steps. We first generated L. monocytogenes and Listeria innocua strains expressing a β-lactamase covalently attached to the bacterial cell wall. These strains were then incubated with HeLa cells containing the Förster resonance energy transfer (FRET) probe CCF4 in their cytoplasm. The CCF4 probe was cleaved by the bacterial surface β-lactamase only in cells inoculated with L. monocytogenes but not those inoculated with L. innocua, thereby demonstrating bacterial access to the host cytoplasm. Subsequently, we performed differential immunofluorescence staining to distinguish extracellular versus total bacterial populations in samples that were also analyzed by the FRET-based assay. With this two-step analysis, bacterial entry can be distinguished from vacuolar rupture in a single experiment. Our novel approach represents a powerful tool for identifying factors that determine the intracellular niche of L. monocytogenes. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  1. Going Vertical To Improve the Accuracy of Atomic Force Microscopy Based Single-Molecule Force Spectroscopy.

    Science.gov (United States)

    Walder, Robert; Van Patten, William J; Adhikari, Ayush; Perkins, Thomas T

    2018-01-23

    Single-molecule force spectroscopy (SMFS) is a powerful technique to characterize the energy landscape of individual proteins, the mechanical properties of nucleic acids, and the strength of receptor-ligand interactions. Atomic force microscopy (AFM)-based SMFS benefits from ongoing progress in improving the precision and stability of cantilevers and the AFM itself. Underappreciated is that the accuracy of such AFM studies remains hindered by inadvertently stretching molecules at an angle while measuring only the vertical component of the force and extension, degrading both measurements. This inaccuracy is particularly problematic in AFM studies using double-stranded DNA and RNA due to their large persistence length (p ≈ 50 nm), often limiting such studies to other SMFS platforms (e.g., custom-built optical and magnetic tweezers). Here, we developed an automated algorithm that aligns the AFM tip above the DNA's attachment point to a coverslip. Importantly, this algorithm was performed at low force (10-20 pN) and relatively fast (15-25 s), preserving the connection between the tip and the target molecule. Our data revealed large uncorrected lateral offsets for 100 and 650 nm DNA molecules [24 ± 18 nm (mean ± standard deviation) and 180 ± 110 nm, respectively]. Correcting this offset yielded a 3-fold improvement in accuracy and precision when characterizing DNA's overstretching transition. We also demonstrated high throughput by acquiring 88 geometrically corrected force-extension curves of a single individual 100 nm DNA molecule in ∼40 min and versatility by aligning polyprotein- and PEG-based protein-ligand assays. Importantly, our software-based algorithm was implemented on a commercial AFM, so it can be broadly adopted. More generally, this work illustrates how to enhance AFM-based SMFS by developing more sophisticated data-acquisition protocols.

  2. Holographic investigation of silver electromigration in nano-sized As2S3 films

    Science.gov (United States)

    Sainov, S.; Todorov, R.; Bodurov, I.; Yovcheva, Temenuzhka

    2013-10-01

    Holographic gratings with a diffraction efficiency (DE) greater than 8% and a spatial resolution of 2237 mm-1 are recorded in very thin As2S3 films with a thickness of 100 nm. Silver photo-diffusion is observed during the holographic recording process while applying a corona discharge. We use the method of holographic grating relaxation spectroscopy (forced Rayleigh scattering) based on the evanescent waves to determine that the silver diffusion coefficient in the thin As2S3 film is in the range of (0.9-10.3) × 10-13 cm2 s-1 depending on the corona charge polarity. This work is dedicated to the 90th anniversary of the birth of Academician Jordan Malinowski.

  3. Real time quantitative phase microscopy based on single-shot transport of intensity equation (ssTIE) method

    Science.gov (United States)

    Yu, Wei; Tian, Xiaolin; He, Xiaoliang; Song, Xiaojun; Xue, Liang; Liu, Cheng; Wang, Shouyu

    2016-08-01

    Microscopy based on transport of intensity equation provides quantitative phase distributions which opens another perspective for cellular observations. However, it requires multi-focal image capturing while mechanical and electrical scanning limits its real time capacity in sample detections. Here, in order to break through this restriction, real time quantitative phase microscopy based on single-shot transport of the intensity equation method is proposed. A programmed phase mask is designed to realize simultaneous multi-focal image recording without any scanning; thus, phase distributions can be quantitatively retrieved in real time. It is believed the proposed method can be potentially applied in various biological and medical applications, especially for live cell imaging.

  4. Statistics on Near Wall Structures and Shear Stress Distribution from 3D Holographic Measurement.

    Science.gov (United States)

    Sheng, J.; Malkiel, E.; Katz, J.

    2007-11-01

    Digital Holographic Microscopy performs 3D velocity measurement in the near-wall region of a turbulent boundary layer in a square channel over a smooth wall at Reτ=1,400. Resolution of ˜1μm over a sample volume of 1.5x2x1.5mm (x^+=50, y^+=60, z^+=50) is sufficient for resolving buffer layer and lower log layer structures, and for measuring instantaneous wall shear stress distributions from velocity gradients in the viscous sublayer. Results, based on 700 instantaneous realizations, provide detailed statistics on the spatial distribution of both wall stress components along with characteristic flow structures. Conditional sampling based on maxima and minima of wall shear stresses, as well as examination of instantaneous flow structures, lead to development of a conceptual model for a characteristic flow phenomenon that seems to generating extreme stress events. This structure develops as an initially spanwise vortex element rises away from the surface, due to local disturbance, causing a local stress minimum. Due to increasing velocity with elevation, this element bends downstream, forming a pair of inclined streamwise vortices, aligned at 45^0 to freestream, with ejection-like flow between them. Entrainment of high streamwise momentum on the outer sides of this vortex pair generates streamwise shear stress maxima, 70 δν downstream, which are displaced laterally by 35 δν from the local minimum.

  5. Holographic complexity and fidelity susceptibility as holographic information dual to different volumes in AdS

    Directory of Open Access Journals (Sweden)

    N.S. Mazhari

    2017-03-01

    Full Text Available The holographic complexity and fidelity susceptibility have been defined as new quantities dual to different volumes in AdS. In this paper, we will use these new proposals to calculate both of these quantities for a variety of interesting deformations of AdS. We obtain the holographic complexity and fidelity susceptibility for an AdS black hole, Janus solution, a solution with cylindrical symmetry, an inhomogeneous background and a hyperscaling violating background. It is observed that the holographic complexity depends on the size of the subsystem for all these solutions and the fidelity susceptibility does not have any such dependence.

  6. Holographic complexity and fidelity susceptibility as holographic information dual to different volumes in AdS

    Energy Technology Data Exchange (ETDEWEB)

    Mazhari, N.S., E-mail: najmemazhari86@gmail.com [Eurasian International Center for Theoretical Physics and Department of General & Theoretical Physics, Eurasian National University, Astana 010008 (Kazakhstan); Momeni, Davood, E-mail: davoodmomeni78@gmail.com [Eurasian International Center for Theoretical Physics and Department of General & Theoretical Physics, Eurasian National University, Astana 010008 (Kazakhstan); Bahamonde, Sebastian, E-mail: sebastian.beltran.14@ucl.ac.uk [Department of Mathematics, University College London, Gower Street, London, WC1E 6BT (United Kingdom); Faizal, Mir, E-mail: mirfaizalmir@googlemail.com [Irving K. Barber School of Arts and Sciences, University of British Columbia - Okanagan, 3333 University Way, Kelowna, British Columbia, V1V 1V7 (Canada); Department of Physics and Astronomy, University of Lethbridge, Lethbridge, Alberta, T1K 3M4 (Canada); Myrzakulov, Ratbay, E-mail: rmyrzakulov@gmail.com [Eurasian International Center for Theoretical Physics and Department of General & Theoretical Physics, Eurasian National University, Astana 010008 (Kazakhstan)

    2017-03-10

    The holographic complexity and fidelity susceptibility have been defined as new quantities dual to different volumes in AdS. In this paper, we will use these new proposals to calculate both of these quantities for a variety of interesting deformations of AdS. We obtain the holographic complexity and fidelity susceptibility for an AdS black hole, Janus solution, a solution with cylindrical symmetry, an inhomogeneous background and a hyperscaling violating background. It is observed that the holographic complexity depends on the size of the subsystem for all these solutions and the fidelity susceptibility does not have any such dependence.

  7. On effective holographic Mott insulators

    Energy Technology Data Exchange (ETDEWEB)

    Baggioli, Matteo; Pujolàs, Oriol [Institut de Física d’Altes Energies (IFAE), Universitat Autònoma de Barcelona,The Barcelona Institute of Science and Technology,Campus UAB, 08193 Bellaterra (Barcelona) (Spain)

    2016-12-20

    We present a class of holographic models that behave effectively as prototypes of Mott insulators — materials where electron-electron interactions dominate transport phenomena. The main ingredient in the gravity dual is that the gauge-field dynamics contains self-interactions by way of a particular type of non-linear electrodynamics. The electrical response in these models exhibits typical features of Mott-like states: i) the low-temperature DC conductivity is unboundedly low; ii) metal-insulator transitions appear by varying various parameters; iii) for large enough self-interaction strength, the conductivity can even decrease with increasing doping (density of carriers) — which appears as a sharp manifestation of ‘traffic-jam’-like behaviour; iv) the insulating state becomes very unstable towards superconductivity at large enough doping. We exhibit some of the properties of the resulting insulator-superconductor transition, which is sensitive to the momentum dissipation rate in a specific way. These models imply a clear and generic correlation between Mott behaviour and significant effects in the nonlinear electrical response. We compute the nonlinear current-voltage curve in our model and find that indeed at large voltage the conductivity is largely reduced.

  8. On effective holographic Mott insulators

    International Nuclear Information System (INIS)

    Baggioli, Matteo; Pujolàs, Oriol

    2016-01-01

    We present a class of holographic models that behave effectively as prototypes of Mott insulators — materials where electron-electron interactions dominate transport phenomena. The main ingredient in the gravity dual is that the gauge-field dynamics contains self-interactions by way of a particular type of non-linear electrodynamics. The electrical response in these models exhibits typical features of Mott-like states: i) the low-temperature DC conductivity is unboundedly low; ii) metal-insulator transitions appear by varying various parameters; iii) for large enough self-interaction strength, the conductivity can even decrease with increasing doping (density of carriers) — which appears as a sharp manifestation of ‘traffic-jam’-like behaviour; iv) the insulating state becomes very unstable towards superconductivity at large enough doping. We exhibit some of the properties of the resulting insulator-superconductor transition, which is sensitive to the momentum dissipation rate in a specific way. These models imply a clear and generic correlation between Mott behaviour and significant effects in the nonlinear electrical response. We compute the nonlinear current-voltage curve in our model and find that indeed at large voltage the conductivity is largely reduced.

  9. Theta dependence in holographic QCD

    Energy Technology Data Exchange (ETDEWEB)

    Bartolini, Lorenzo [Dipartimento di Fisica “E. Fermi' , Università di Pisa and INFN, Sezione di Pisa,Largo B. Pontecorvo 3, I-56127 Pisa (Italy); Bigazzi, Francesco [INFN, Sezione di Firenze,Via G. Sansone 1, I-50019 Sesto Fiorentino (Firenze) (Italy); Bolognesi, Stefano [Dipartimento di Fisica “E. Fermi' , Università di Pisa and INFN, Sezione di Pisa,Largo B. Pontecorvo 3, I-56127 Pisa (Italy); Cotrone, Aldo L. [INFN, Sezione di Firenze,Via G. Sansone 1, I-50019 Sesto Fiorentino (Firenze) (Italy); Dipartimento di Fisica e Astronomia, Università di Firenze,Via G. Sansone 1, I-50019 Sesto Fiorentino (Firenze) (Italy); Manenti, Andrea [Institute of Physics, EPFL,Rte de la Sorge, BSP 728, CH-1015 Lausanne (Switzerland)

    2017-02-07

    We study the effects of the CP-breaking topological θ-term in the large N{sub c} QCD model by Witten, Sakai and Sugimoto with N{sub f} degenerate light flavors. We first compute the ground state energy density, the topological susceptibility and the masses of the lowest lying mesons, finding agreement with expectations from the QCD chiral effective action. Then, focusing on the N{sub f}=2 case, we consider the baryonic sector and determine, to leading order in the small θ regime, the related holographic instantonic soliton solutions. We find that while the baryon spectrum does not receive O(θ) corrections, this is not the case for observables like the electromagnetic form factor of the nucleons. In particular, it exhibits a dipole term, which turns out to be vector-meson dominated. The resulting neutron electric dipole moment, which is exactly the opposite as that of the proton, is of the same order of magnitude of previous estimates in the literature. Finally, we compute the CP-violating pion-nucleon coupling constant ḡ{sub πNN}, finding that it is zero to leading order in the large N{sub c} limit.

  10. Holographic cutoff on inflationary universes

    International Nuclear Information System (INIS)

    Santos, Fabio M. de N.; Cunha, Bruno Carneiro da

    2011-01-01

    Full text: Cosmological Inflation has been widely accepted as the standard explanation of the onset of Big-Bang Cosmology. However, many critiques have been made about the lack of an account of quantum gravity degrees of freedom in cosmology. There is no definite consensus in the literature if we should consider the influence of pre-Plackian modes, for example, in inflationary models. We propose here a general approach to take quantum gravity into account by imposing a holographic cutoff on the number of states of cosmological theories. We apply the method to inflationary scalar field models coupled to a generic potential V (φ). This thermodynamic cutoff allow us to assess the relative volume of phase space which inflates for the particular model where V (φ) = m 2 φ 2 /2. The density of states of the model is defined by taking the coincidence limit of the Hadamard Green function G (1) and we use the point-splitting method to regulate the expression. Our conclusion is that inflation has probability very close to one. (author)

  11. Linearity of holographic entanglement entropy

    Energy Technology Data Exchange (ETDEWEB)

    Almheiri, Ahmed [Stanford Institute for Theoretical Physics, Department of Physics,Stanford University, Stanford, CA 94305 (United States); Dong, Xi [School of Natural Sciences, Institute for Advanced Study,Princeton, NJ 08540 (United States); Swingle, Brian [Stanford Institute for Theoretical Physics, Department of Physics,Stanford University, Stanford, CA 94305 (United States)

    2017-02-14

    We consider the question of whether the leading contribution to the entanglement entropy in holographic CFTs is truly given by the expectation value of a linear operator as is suggested by the Ryu-Takayanagi formula. We investigate this property by computing the entanglement entropy, via the replica trick, in states dual to superpositions of macroscopically distinct geometries and find it consistent with evaluating the expectation value of the area operator within such states. However, we find that this fails once the number of semi-classical states in the superposition grows exponentially in the central charge of the CFT. Moreover, in certain such scenarios we find that the choice of surface on which to evaluate the area operator depends on the density matrix of the entire CFT. This nonlinearity is enforced in the bulk via the homology prescription of Ryu-Takayanagi. We thus conclude that the homology constraint is not a linear property in the CFT. We also discuss the existence of ‘entropy operators’ in general systems with a large number of degrees of freedom.

  12. Development of a laser-based heating system for in situ synchrotron-based X-ray tomographic microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Fife, Julie L., E-mail: julie.fife@psi.ch [Laboratory for Synchrotron Radiation, Swiss Light Source, Paul Scherrer Institut, Villigen (Switzerland); Computational Materials Laboratory, Ecole Polytechnique Federale de Lausanne, Lausanne (Switzerland); Rappaz, Michel [Computational Materials Laboratory, Ecole Polytechnique Federale de Lausanne, Lausanne (Switzerland); Pistone, Mattia [Institute for Geochemistry and Petrology, Swiss Federal Institute of Technology of Zurich, Zurich (Switzerland); Celcer, Tine [Laboratory for Synchrotron Radiation, Swiss Light Source, Paul Scherrer Institut, Villigen (Switzerland); The Centre of Excellence for Biosensors, Instrumentation and Process Control, Solkan (Slovenia); Mikuljan, Gordan [Laboratory for Synchrotron Radiation, Swiss Light Source, Paul Scherrer Institut, Villigen (Switzerland); Stampanoni, Marco [Laboratory for Synchrotron Radiation, Swiss Light Source, Paul Scherrer Institut, Villigen (Switzerland); Institute for Biomedical Engineering, Swiss Federal Institute of Technology and University of Zurich, Zurich (Switzerland)

    2012-05-01

    A laser-based heating system has been developed at the TOMCAT beamline of the Swiss Light Source for in situ observations of moderate-to-high-temperature applications of materials. Understanding the formation of materials at elevated temperatures is critical for determining their final properties. Synchrotron-based X-ray tomographic microscopy is an ideal technique for studying such processes because high spatial and temporal resolutions are easily achieved and the technique is non-destructive, meaning additional analyses can take place after data collection. To exploit the state-of-the-art capabilities at the tomographic microscopy and coherent radiology experiments (TOMCAT) beamline of the Swiss Light Source, a general-use moderate-to-high-temperature furnace has been developed. Powered by two diode lasers, it provides controlled localized heating, from 673 to 1973 K, to examine many materials systems and their dynamics in real time. The system can also be operated in various thermal modalities. For example, near-isothermal conditions at a given sample location can be achieved with a prescribed time-dependent temperature. This mode is typically used to study isothermal phase transformations; for example, the formation of equiaxed grains in metallic systems or to nucleate and grow bubble foams in silicate melts under conditions that simulate volcanic processes. In another mode, the power of the laser can be fixed and the specimen moved at a constant speed in a user-defined thermal gradient. This is similar to Bridgman solidification, where the thermal gradient and cooling rate control the microstructure formation. This paper details the experimental set-up and provides multiple proofs-of-concept that illustrate the versatility of using this laser-based heating system to explore, in situ, many elevated-temperature phenomena in a variety of materials.

  13. Holographic dark energy from fluid/gravity duality constraint by cosmological observations

    Science.gov (United States)

    Pourhassan, Behnam; Bonilla, Alexander; Faizal, Mir; Abreu, Everton M. C.

    2018-06-01

    In this paper, we obtain a holographic model of dark energy using the fluid/gravity duality. This model will be dual to a higher dimensional Schwarzschild black hole, and we would use fluid/gravity duality to relate to the parameters of this black hole to such a cosmological model. We will also analyze the thermodynamics of such a solution, and discuss the stability model. Finally, we use cosmological data to constraint the parametric space of this dark energy model. Thus, we will use observational data to perform cosmography for this holographic model based on fluid/gravity duality.

  14. HoloHands: games console interface for controlling holographic optical manipulation

    Science.gov (United States)

    McDonald, C.; McPherson, M.; McDougall, C.; McGloin, D.

    2013-03-01

    The increasing number of applications for holographic manipulation techniques has sparked the development of more accessible control interfaces. Here, we describe a holographic optical tweezers experiment which is controlled by gestures that are detected by a Microsoft Kinect. We demonstrate that this technique can be used to calibrate the tweezers using the Stokes drag method and compare this to automated calibrations. We also show that multiple particle manipulation can be handled. This is a promising new line of research for gesture-based control which could find applications in a wide variety of experimental situations.

  15. HoloHands: games console interface for controlling holographic optical manipulation

    International Nuclear Information System (INIS)

    McDonald, C; McPherson, M; McDougall, C; McGloin, D

    2013-01-01

    The increasing number of applications for holographic manipulation techniques has sparked the development of more accessible control interfaces. Here, we describe a holographic optical tweezers experiment which is controlled by gestures that are detected by a Microsoft Kinect. We demonstrate that this technique can be used to calibrate the tweezers using the Stokes drag method and compare this to automated calibrations. We also show that multiple particle manipulation can be handled. This is a promising new line of research for gesture-based control which could find applications in a wide variety of experimental situations. (paper)

  16. Acoustical holographic recording with coherent optical read-out and image processing

    Science.gov (United States)

    Liu, H. K.

    1980-10-01

    New acoustic holographic wave memory devices have been designed for real-time in-situ recording applications. The basic operating principles of these devices and experimental results through the use of some of the prototypes of the devices are presented. Recording media used in the device include thermoplastic resin, Crisco vegetable oil, and Wilson corn oil. In addition, nonlinear coherent optical image processing techniques including equidensitometry, A-D conversion, and pseudo-color, all based on the new contact screen technique, are discussed with regard to the enhancement of the normally poor-resolved acoustical holographic images.

  17. The bacterial rhizobiome of hyperaccumulators: future perspectives based on omics analysis and advanced microscopy

    Directory of Open Access Journals (Sweden)

    Giovanna eVisioli

    2015-01-01

    Full Text Available Hyperaccumulators are plants that can extract heavy metal ions from the soil and translocate those ions to the shoots, where they are sequestered and detoxified. Hyperaccumulation depends not only on the availability of mobilized metal ions in the soil, but also on the enhanced activity of metal transporters and metal chelators which may be provided by the plant or its associated microbes. The rhizobiome is captured by plant root exudates from the complex microbial community in the soil, and may colonize the root surface or infiltrate the root cortex. This community can increase the root surface area by inducing hairy root proliferation. It may also increase the solubility of metals in the rhizosphere and promote the uptake of soluble metals by the plant. The bacterial rhizobiome, a subset of specialized microorganisms that colonize the plant rhizosphere and endosphere, makes an important contribution to the hyperaccumulator phenotype. In this review, we discuss classic and more recent tools that are used to study the interactions between hyperaccumulators and the bacterial rhizobiome, and consider future perspectives based on the use of omics analysis and microscopy to study plant metabolism in the context of metal accumulation. Recent data suggest that metal-resistant bacteria isolated from the hyperaccumulator rhizosphere and endosphere could be useful in applications such as phytoextraction and phytoremediation, although more research is required to determine whether such properties can be transferred successfully to non-accumulator species.

  18. Identifying Two Common Types of Breast Benign Diseases Based on Multiphoton Microscopy

    Directory of Open Access Journals (Sweden)

    Yan Wu

    2018-01-01

    Full Text Available Multiphoton microscopy has attracted increasing attention and investigations in the field of breast cancer, based on two-photon excited fluorescence (TPEF and second-harmonic generation (SHG. However, the incidence of breast benign diseases is about 5 to 10 times higher than breast cancer; up to 30% of women suffer from breast benign diseases and require treatment at some time in their lives. Thus, in this study, MPM was applied to image fibroadenoma and fibrocystic lesion, which are two of the most common breast benign diseases. The results show that MPM has the capability to identify the microstructure of lobule and stroma in normal breast tissue, the interaction of compressed ducts with surrounding collagen fiber in fibroadenoma, and the architecture of cysts filled with cystic fluid in fibrocystic disease. These findings indicate that, with integration of MPM into currently accepted clinical imaging system, it has the potential to make a real-time diagnosis of breast benign diseases in vivo, as well as breast cancer.

  19. Scanning Auger microscopy study of lanthanum partitioning in sphene-based glass-ceramics

    International Nuclear Information System (INIS)

    Hocking, W.H.; Hayward, P.J.; Watson, D.G.; Allen, G.C.

    1984-01-01

    Glass-ceramics are being investigated as possible hosts for the radioactive wastes that would result from recycling irradiated nuclear fuels. The partitioning of lanthanum in sphene-based glass-ceramics has been studied by scanning Auger electron microscopy for lanthanum concentrations from 0.2 to 2.0 mol.%. Sphene crystals (CaTiSiO 5 ) were located in the silica-rich glass matrix by recording digital Auger images of the calcium and titanium distributions. The sphene crystals were typically 0.5 to 5 μm in size and occupied approximately 40% of the total specimen volume. Auger spot analyses revealed that lanthanum was strongly partitioned into the sphene phase of phosphorus-free glass-ceramics; however, when a small amount of phosphorus was included in the glass-ceramic composition as a crystal nucleating agent, the lanthanum was concentrated in a third minor phase which also contained calcium, phosphorus and oxygen. Chemical shift effects in the Auger spectra of silicon, titanium and phosphorus showed evidence for electron-stimulated desorption of oxygen. (author)

  20. Development of an automated asbestos counting software based on fluorescence microscopy.

    Science.gov (United States)

    Alexandrov, Maxym; Ichida, Etsuko; Nishimura, Tomoki; Aoki, Kousuke; Ishida, Takenori; Hirota, Ryuichi; Ikeda, Takeshi; Kawasaki, Tetsuo; Kuroda, Akio

    2015-01-01

    An emerging alternative to the commonly used analytical methods for asbestos analysis is fluorescence microscopy (FM), which relies on highly specific asbestos-binding probes to distinguish asbestos from interfering non-asbestos fibers. However, all types of microscopic asbestos analysis require laborious examination of large number of fields of view and are prone to subjective errors and large variability between asbestos counts by different analysts and laboratories. A possible solution to these problems is automated counting of asbestos fibers by image analysis software, which would lower the cost and increase the reliability of asbestos testing. This study seeks to develop a fiber recognition and counting software for FM-based asbestos analysis. We discuss the main features of the developed software and the results of its testing. Software testing showed good correlation between automated and manual counts for the samples with medium and high fiber concentrations. At low fiber concentrations, the automated counts were less accurate, leading us to implement correction mode for automated counts. While the full automation of asbestos analysis would require further improvements in accuracy of fiber identification, the developed software could already assist professional asbestos analysts and record detailed fiber dimensions for the use in epidemiological research.

  1. Pattern of glomerular diseases in oman: A study based on light microscopy and immunofluorescence

    Directory of Open Access Journals (Sweden)

    Nasar Yousuf Alwahaibi

    2013-01-01

    Full Text Available Light microscopy and immunofluorescence play an important part in the final diagnosis of renal biopsy. The aim of this study was to analyze the pattern of various glomerular diseases in Oman. A total of 424 renal biopsies were retrospectively analyzed at the Sultan Qaboos University Hospital between 1999 and 2010. Focal and segmental glomerulosclerosis (FSGS, minimal change disease (MCD, membranous glomerulopathy (MGN and IgA nephropathy were the most common primary glomerular diseases encountered, accounting for 21.2%, 17%, 12.3% and 8.3%, respectively, of all cases. Lupus nephritis was the most common secondary glomerular disease and was the most prevalent among all biopsies, accounting for 30.4% of all biopsies. Amyloidosis was seen in only two cases. The presence of fluorescein isothiocyanatefibrin in all renal cases was low when compared with IgG, IgA, IgM, C3 and C1q markers. In conclusion, based on the findings of this study, lupus nephritis was the most common of all glomerular diseases and FSGS was the most common primary glomerular disease. The importance of fluorescein isothiocyanate-fibrin in the diagnosis of renal biopsy needs to be further investigated.

  2. A confocal microscopy-based atlas of tissue architecture in the tapeworm Hymenolepis diminuta.

    Science.gov (United States)

    Rozario, Tania; Newmark, Phillip A

    2015-11-01

    Tapeworms are pervasive and globally distributed parasites that infect millions of humans and livestock every year, and are the causative agents of two of the 17 neglected tropical diseases prioritized by the World Health Organization. Studies of tapeworm biology and pathology are often encumbered by the complex life cycles of disease-relevant tapeworm species that infect hosts such as foxes, dogs, cattle, pigs, and humans. Thus, studies of laboratory models can help overcome the practical, ethical, and cost-related difficulties faced by tapeworm parasitologists. The rat intestinal tapeworm Hymenolepis diminuta is easily reared in the laboratory and has the potential to enable modern molecular-based experiments that will greatly contribute to our understanding of multiple aspects of tapeworm biology, such as growth and reproduction. As part of our efforts to develop molecular tools for experiments on H. diminuta, we have characterized a battery of lectins, antibodies, and common stains that label different tapeworm tissues and organ structures. Using confocal microscopy, we have assembled an "atlas" of H. diminuta organ architecture that will be a useful resource for helminthologists. The methodologies we describe will facilitate characterization of loss-of-function perturbations using H. diminuta. This toolkit will enable a greater understanding of fundamental tapeworm biology that may elucidate new therapeutic targets toward the eradication of these parasites. Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

  3. Unconventional methods of imaging: computational microscopy and compact implementations

    Science.gov (United States)

    McLeod, Euan; Ozcan, Aydogan

    2016-07-01

    In the past two decades or so, there has been a renaissance of optical microscopy research and development. Much work has been done in an effort to improve the resolution and sensitivity of microscopes, while at the same time to introduce new imaging modalities, and make existing imaging systems more efficient and more accessible. In this review, we look at two particular aspects of this renaissance: computational imaging techniques and compact imaging platforms. In many cases, these aspects go hand-in-hand because the use of computational techniques can simplify the demands placed on optical hardware in obtaining a desired imaging performance. In the first main section, we cover lens-based computational imaging, in particular, light-field microscopy, structured illumination, synthetic aperture, Fourier ptychography, and compressive imaging. In the second main section, we review lensfree holographic on-chip imaging, including how images are reconstructed, phase recovery techniques, and integration with smart substrates for more advanced imaging tasks. In the third main section we describe how these and other microscopy modalities have been implemented in compact and field-portable devices, often based around smartphones. Finally, we conclude with some comments about opportunities and demand for better results, and where we believe the field is heading.

  4. Analyzer-based x-ray phase-contrast microscopy combining channel-cut and asymmetrically cut crystals

    International Nuclear Information System (INIS)

    Hoennicke, M. G.; Cusatis, C.

    2007-01-01

    An analyzer-based x-ray phase-contrast microscopy (ABM) setup combining a standard analyzer-based x-ray phase-contrast imaging (ABI) setup [nondispersive 4-crystal setup (Bonse-Hart setup)] and diffraction by asymmetrically cut crystals is presented here. An attenuation-contrast microscopy setup with conventional x-ray source and asymmetrically cut crystals is first analyzed. Edge-enhanced effects attributed to phase jumps or refraction/total external reflection on the fiber borders were detected. However, the long exposure times and the possibility to achieve high contrast microscopies by using extremely low attenuation-contrast samples motivated us to assemble the ABM setup using a synchrotron source. This setup was found to be useful for low contrast attenuation samples due to the low exposure time, high contrast, and spatial resolution found. Moreover, thanks to the combination with the nondispersive ABI setup, the diffraction-enhanced x-ray imaging algorithm could be applied

  5. Holographic duality in condensed matter physics

    CERN Document Server

    Zaanen, Jan; Sun, Ya-Wen; Schalm, Koenraad

    2015-01-01

    A pioneering treatise presenting how the new mathematical techniques of holographic duality unify seemingly unrelated fields of physics. This innovative development morphs quantum field theory, general relativity and the renormalisation group into a single computational framework and this book is the first to bring together a wide range of research in this rapidly developing field. Set within the context of condensed matter physics and using boxes highlighting the specific techniques required, it examines the holographic description of thermal properties of matter, Fermi liquids and superconductors, and hitherto unknown forms of macroscopically entangled quantum matter in terms of general relativity, stars and black holes. Showing that holographic duality can succeed where classic mathematical approaches fail, this text provides a thorough overview of this major breakthrough at the heart of modern physics. The inclusion of extensive introductory material using non-technical language and online Mathematica not...

  6. The holographic Weyl semi-metal

    Directory of Open Access Journals (Sweden)

    Karl Landsteiner

    2016-02-01

    Full Text Available We present a holographic model of a Weyl semi-metal. We show the evidences that upon varying a mass parameter the model undergoes a sharp crossover at small temperature from a topologically non-trivial state to a trivial one. The order parameter is the anomalous Hall effect (AHE and we find that it is very strongly suppressed above a critical value of the mass parameter. This can be taken as a hint for an underlying topological quantum phase transition. We give an interpretation of the results in terms of a holographic RG flow and compare to a weakly coupled field theoretical model. Since there are no fermionic quasiparticle excitations in the strongly coupled holographic model the presence of an anomalous Hall effect cannot be bound to notions of topology in momentum spaces.

  7. The holographic Weyl semi-metal

    Energy Technology Data Exchange (ETDEWEB)

    Landsteiner, Karl, E-mail: karl.landsteiner@csic.es; Liu, Yan, E-mail: yan.liu@csic.es

    2016-02-10

    We present a holographic model of a Weyl semi-metal. We show the evidences that upon varying a mass parameter the model undergoes a sharp crossover at small temperature from a topologically non-trivial state to a trivial one. The order parameter is the anomalous Hall effect (AHE) and we find that it is very strongly suppressed above a critical value of the mass parameter. This can be taken as a hint for an underlying topological quantum phase transition. We give an interpretation of the results in terms of a holographic RG flow and compare to a weakly coupled field theoretical model. Since there are no fermionic quasiparticle excitations in the strongly coupled holographic model the presence of an anomalous Hall effect cannot be bound to notions of topology in momentum spaces.

  8. Holographic correlation functions in Critical Gravity

    Science.gov (United States)

    Anastasiou, Giorgos; Olea, Rodrigo

    2017-11-01

    We compute the holographic stress tensor and the logarithmic energy-momentum tensor of Einstein-Weyl gravity at the critical point. This computation is carried out performing a holographic expansion in a bulk action supplemented by the Gauss-Bonnet term with a fixed coupling. The renormalization scheme defined by the addition of this topological term has the remarkable feature that all Einstein modes are identically cancelled both from the action and its variation. Thus, what remains comes from a nonvanishing Bach tensor, which accounts for non-Einstein modes associated to logarithmic terms which appear in the expansion of the metric. In particular, we compute the holographic 1-point functions for a generic boundary geometric source.

  9. Collapse and revival in holographic quenches

    International Nuclear Information System (INIS)

    Silva, Emilia da; Lopez, Esperanza; Mas, Javier; Serantes, Alexandre

    2015-01-01

    We study holographic models related to global quantum quenches in finite size systems. The holographic set up describes naturally a CFT, which we consider on a circle and a sphere. The enhanced symmetry of the conformal group on the circle motivates us to compare the evolution in both cases. Depending on the initial conditions, the dual geometry exhibits oscillations that we holographically interpret as revivals of the initial field theory state. On the sphere, this only happens when the energy density created by the quench is small compared to the system size. However on the circle considerably larger energy densities are compatible with revivals. Two different timescales emerge in this latter case. A collapse time, when the system appears to have dephased, and the revival time, when after rephasing the initial state is partially recovered. The ratio of these two times depends upon the initial conditions in a similar way to what is observed in some experimental setups exhibiting collapse and revivals.

  10. Holographic dark energy in the DGP model

    International Nuclear Information System (INIS)

    Cruz, Norman; Lepe, Samuel; Pena, Francisco; Avelino, Arturo

    2012-01-01

    The braneworld model proposed by Dvali, Gabadadze, and Porrati leads to an accelerated universe without cosmological constant or any other form of dark energy. Nevertheless, we have investigated the consequences of this model when an holographic dark energy is included, taking the Hubble scale as IR cutoff. We have found that the holographic dark energy leads to an accelerated flat universe (de Sitter-like expansion) for the two branches: ε=±1, of the DGP model. Nevertheless, in universes with no null curvature the dark energy presents an EoS corresponding to a phantom fluid during the present era and evolving to a de Sitter-like phase for future cosmic time. In the special case in which the holographic parameter c is equal to one we have found a sudden singularity in closed universes. In this case the expansion is decelerating. (orig.)

  11. Holographic dark energy in the DGP model

    Energy Technology Data Exchange (ETDEWEB)

    Cruz, Norman [Universidad de Santiago, Departamento de Fisica, Facultad de Ciencia, Santiago (Chile); Lepe, Samuel [Pontificia Universidad Catolica de Valparaiso, Instituto de Fisica, Facultad de Ciencias, Valparaiso (Chile); Pena, Francisco [Universidad de La Frontera, Departamento de Ciencias Fisicas, Facultad de Ingenieria, Ciencias y Administracion, Avda. Francisco Salazar 01145, Casilla 54-D, Temuco (Chile); Avelino, Arturo [Universidad de Guanajuato, Departamento de Fisica, DCI, Codigo Postal 37150, Leon, Guanajuato (Mexico)

    2012-09-15

    The braneworld model proposed by Dvali, Gabadadze, and Porrati leads to an accelerated universe without cosmological constant or any other form of dark energy. Nevertheless, we have investigated the consequences of this model when an holographic dark energy is included, taking the Hubble scale as IR cutoff. We have found that the holographic dark energy leads to an accelerated flat universe (de Sitter-like expansion) for the two branches: {epsilon}={+-}1, of the DGP model. Nevertheless, in universes with no null curvature the dark energy presents an EoS corresponding to a phantom fluid during the present era and evolving to a de Sitter-like phase for future cosmic time. In the special case in which the holographic parameter c is equal to one we have found a sudden singularity in closed universes. In this case the expansion is decelerating. (orig.)

  12. Holographic subregion complexity for singular surfaces

    Energy Technology Data Exchange (ETDEWEB)

    Bakhshaei, Elaheh [Isfahan University of Technology, Department of Physics, Isfahan (Iran, Islamic Republic of); Mollabashi, Ali [Institute for Research in Fundamental Sciences (IPM), School of Physics, Tehran (Iran, Islamic Republic of); Shirzad, Ahmad [Isfahan University of Technology, Department of Physics, Isfahan (Iran, Islamic Republic of); Institute for Research in Fundamental Sciences (IPM), School of Particles and Accelerators, Tehran (Iran, Islamic Republic of)

    2017-10-15

    Recently holographic prescriptions were proposed to compute the quantum complexity of a given state in the boundary theory. A specific proposal known as 'holographic subregion complexity' is supposed to calculate the complexity of a reduced density matrix corresponding to a static subregion. We study different families of singular subregions in the dual field theory and find the divergence structure and universal terms of holographic subregion complexity for these singular surfaces. We find that there are new universal terms, logarithmic in the UV cut-off, due to the singularities of a family of surfaces including a kink in (2 + 1) dimensions and cones in even dimensional field theories. We also find examples of new divergent terms such as squared logarithm and negative powers times the logarithm of the UV cut-off parameter. (orig.)

  13. Thin combiner optics utilizing volume holographic optical elements (vHOEs) using Bayfol HX photopolymer film

    Science.gov (United States)

    Bruder, Friedrich-Karl; Fäcke, Thomas; Hagen, Rainer; Hansen, Sven; Manecke, Christel; Orselli, Enrico; Rewitz, Christian; Rölle, Thomas; Walze, Günther

    2017-06-01

    The main function of any augmented reality system is to seamlessly merge the real world perception of a viewer with computer generated images and information. Besides real-time head-tracking and room-scanning capabilities the combiner optics, which optically merge the natural with the artificial visual information, represent a key component for those systems. Various types of combiner optics are known to the industry, all with their specific advantages and disadvantages. Beside the well-established solutions based on refractive optics or surface gratings, volume Holographic Optical Elements (vHOEs) are a very attractive alternative in this field. The unique characteristics of these diffractive grating structures - being lightweight, thin, flat and invisible in Off Bragg conditions - make them perfectly suitable for their use in integrated and compact combiners. For any consumer application it is paramount to build unobtrusive and lightweight augmented reality displays, for which those volume holographic combiners are ideally suited. Due to processing challenges of (historic) holographic recording materials mass production of vHOE holographic combiners was not possible. Therefore vHOE based combiners found use in military applications only by now. The new Bayfol® HX instant developing holographic photopolymer film provides an ideal technology platform to optimize the performance of vHOEs in a wide range of applications. Bayfol® HX provides full color capability and adjustable diffraction efficiency as well as an unprecedented optical clarity when compared to classical holographic recording materials like silver halide emulsions (AgHX) or dichromated gelatin (DCG). Bayfol® HX film is available in industrial scale and quality. Its properties can be tailored for various diffractive performances and integration methods. Bayfol® HX film is easy to process without any need for chemical or thermal development steps, offering simplified contact-copy mass production

  14. Transmission electron microscopy of GaN based, doped semiconductor heterostructures

    Energy Technology Data Exchange (ETDEWEB)

    Pretorius, A.

    2006-07-01

    This thesis addresses the analysis of GaN based heterostructures with transmission electron microscopy (TEM). Basic properties of the material of interest are introduced in chapter 2. These include the structural and optical properties as well as an introduction to the growth methods used for the samples analysed in this work. In chapter 3 a brief theoretical treatment of TEM is given. As one main topic of this work is the determination of the In concentration in InGaN islands using strain state analysis, a detailed description of the method is given. Chapter 4 describes the results obtained for pyramidal defects present in metalorganic vapour phase epitaxy grown GaN:Mg with high dopant concentration. Based on the experimental results and the well established knowledge that GaN of inverted polarity is present inside the pyramidal defects, a variety of basal plane inversion domain boundary models was set up. From these models, HRTEM images were simulated using the multislice approach, followed by a quantitative comparison to experimentally obtained HRTEM images. Another focus of this work is the analysis of In{sub x}Ga{sub 1-x}N islands grown on GaN presented in chapter 5. Following a literature survey which describes different methods used to obtain In{sub x}Ga{sub 1-x}N islands, the first topic is the distinction of In{sub x}Ga{sub 1-x}N islands and metal droplets, which can form during growth. This is followed by the experimental results of molecular beam epitaxy and metalorganic vapour phase epitaxy grown In{sub x}Ga{sub 1-x}N island and quantum dot samples. (orig.)

  15. Bulk viscosity in holographic Lifshitz hydrodynamics

    International Nuclear Information System (INIS)

    Hoyos, Carlos; Kim, Bom Soo; Oz, Yaron

    2014-01-01

    We compute the bulk viscosity in holographic models dual to theories with Lifshitz scaling and/or hyperscaling violation, using a generalization of the bulk viscosity formula derived in arXiv:1103.1657 from the null focusing equation. We find that only a class of models with massive vector fields are truly Lifshitz scale invariant, and have a vanishing bulk viscosity. For other holographic models with scalars and/or massless vector fields we find a universal formula in terms of the dynamical exponent and the hyperscaling violation exponent

  16. Numerical processing of ultrasonic holographic data

    International Nuclear Information System (INIS)

    Langenberg, K.J.; Kiefer, R.; Wosnitza, M.; Schmitz, V.; Fraunhofer-Gesellschaft zur Foerderung der Angewandten Forschung e.V., Saarbruecken

    1980-01-01

    Reconstructing ultrasonic holographic data numerically, the well-known Fresnel approximation is a first step in evaluating the Rayleigh-Sommerfeld diffraction formula, that is to say, a one- or two-dimensional Fourier-transform of the holographic data multiplied by a complex phase factor has to be computed. The present contribution investigates the relation between flaw depth and aperture size yielding the more advantageous use of the spatial frequency approach where the advantage is in terms of the number of samples and hence computation time in evaluating Fourier transforms numerically. (orig.) [de

  17. Holographic corrections to meson scattering amplitudes

    Energy Technology Data Exchange (ETDEWEB)

    Armoni, Adi; Ireson, Edwin, E-mail: 746616@swansea.ac.uk

    2017-06-15

    We compute meson scattering amplitudes using the holographic duality between confining gauge theories and string theory, in order to consider holographic corrections to the Veneziano amplitude and associated higher-point functions. The generic nature of such computations is explained, thanks to the well-understood nature of confining string backgrounds, and two different examples of the calculation in given backgrounds are used to illustrate the details. The effect we discover, whilst only qualitative, is re-obtainable in many such examples, in four-point but also higher point amplitudes.

  18. Real-time wideband holographic surveillance system

    Science.gov (United States)

    Sheen, D.M.; Collins, H.D.; Hall, T.E.; McMakin, D.L.; Gribble, R.P.; Severtsen, R.H.; Prince, J.M.; Reid, L.D.

    1996-09-17

    A wideband holographic surveillance system including a transceiver for generating a plurality of electromagnetic waves; antenna for transmitting the electromagnetic waves toward a target at a plurality of predetermined positions in space; the transceiver also receiving and converting electromagnetic waves reflected from the target to electrical signals at a plurality of predetermined positions in space; a computer for processing the electrical signals to obtain signals corresponding to a holographic reconstruction of the target; and a display for displaying the processed information to determine nature of the target. The computer has instructions to apply a three dimensional backward wave algorithm. 28 figs.

  19. Holographic Aspects of a Relativistic Nonconformal Theory

    Directory of Open Access Journals (Sweden)

    Chanyong Park

    2013-01-01

    Full Text Available We study a general D-dimensional Schwarzschild-type black brane solution of the Einstein-dilaton theory and derive, by using the holographic renormalization, its thermodynamics consistent with the geometric results. Using the membrane paradigm, we calculate the several hydrodynamic transport coefficients and compare them with the results obtained by the Kubo formula, which shows the self-consistency of the gauge/gravity duality in the relativistic nonconformal theory. In order to understand more about the relativistic non-conformal theory, we further investigate the binding energy, drag force, and holographic entanglement entropy of the relativistic non-conformal theory.

  20. Interacting holographic dark energy with logarithmic correction

    International Nuclear Information System (INIS)

    Jamil, Mubasher; Farooq, M. Umar

    2010-01-01

    The holographic dark energy (HDE) is considered to be the most promising candidate of dark energy. Its definition is motivated from the entropy-area relation which depends on the theory of gravity under consideration. Recently a new definition of HDE is proposed with the help of quantum corrections to the entropy-area relation in the setup of loop quantum cosmology. Employing this new definition, we investigate the model of interacting dark energy and derive its effective equation of state. Finally we establish a correspondence between generalized Chaplygin gas and entropy-corrected holographic dark energy

  1. Entropy-Corrected Holographic Dark Energy

    International Nuclear Information System (INIS)

    Wei Hao

    2009-01-01

    The holographic dark energy (HDE) is now an interesting candidate of dark energy, which has been studied extensively in the literature. In the derivation of HDE, the black hole entropy plays an important role. In fact, the entropy-area relation can be modified due to loop quantum gravity or other reasons. With the modified entropy-area relation, we propose the so-called 'entropy-corrected holographic dark energy' (ECHDE) in the present work. We consider many aspects of ECHDE and find some interesting results. In addition, we briefly consider the so-called 'entropy-corrected agegraphic dark energy' (ECADE). (geophysics, astronomy, and astrophysics)

  2. Fine Metal Mask 3-Dimensional Measurement by using Scanning Digital Holographic Microscope

    Science.gov (United States)

    Shin, Sanghoon; Yu, Younghun

    2018-04-01

    For three-dimensional microscopy, fast and high axial resolution are very important. Extending the depth of field for digital holographic is necessary for three-dimensional measurements of thick samples. We propose an optical sectioning method for optical scanning digital holography that is performed in the frequency domain by spatial filtering of a reconstructed amplitude image. We established a scanning dual-wavelength off-axis digital holographic microscope to measure samples that exhibit a large amount of coherent noise and a thickness larger than the depth of focus of the objective lens. As a demonstration, we performed a three-dimensional measurement of a fine metal mask with a reconstructed sectional phase image and filtering with a reconstructed amplitude image.

  3. COMPARISON OF HOLOGRAPHIC AND ITERATIVE METHODS FOR AMPLITUDE OBJECT RECONSTRUCTION

    Directory of Open Access Journals (Sweden)

    I. A. Shevkunov

    2015-01-01

    Full Text Available Experimental comparison of four methods for the wavefront reconstruction is presented. We considered two iterative and two holographic methods with different mathematical models and algorithms for recovery. The first two of these methods do not use a reference wave recording scheme that reduces requirements for stability of the installation. A major role in phase information reconstruction by such methods is played by a set of spatial intensity distributions, which are recorded as the recording matrix is being moved along the optical axis. The obtained data are used consistently for wavefront reconstruction using an iterative procedure. In the course of this procedure numerical distribution of the wavefront between the planes is performed. Thus, phase information of the wavefront is stored in every plane and calculated amplitude distributions are replaced for the measured ones in these planes. In the first of the compared methods, a two-dimensional Fresnel transform and iterative calculation in the object plane are used as a mathematical model. In the second approach, an angular spectrum method is used for numerical wavefront propagation, and the iterative calculation is carried out only between closely located planes of data registration. Two digital holography methods, based on the usage of the reference wave in the recording scheme and differing from each other by numerical reconstruction algorithm of digital holograms, are compared with the first two methods. The comparison proved that the iterative method based on 2D Fresnel transform gives results comparable with the result of common holographic method with the Fourier-filtering. It is shown that holographic method for reconstructing of the object complex amplitude in the process of the object amplitude reduction is the best among considered ones.

  4. Cholesteric lyomesophases based on sodium N-lauroyl asparte: characterization of new system by nuclear magnetic resonance and polarizing microscopy

    International Nuclear Information System (INIS)

    Melo, M.V.M.C. de.

    1982-01-01

    Lyomesophases based on di-sodium N-lauroyl aspartate (SNLA), bi-carboxilated amphiphile obtained from the reaction of n-lauroyl chloride with aspartic acid in racemic or levo form are studies. The different mesophases were characterized by 2 H and 23 Na NMR and by polarizing microscopy. (M.J.C.) [pt

  5. Development and validation of an automated, microscopy-based method for enumeration of groups of intestinal bacteria

    NARCIS (Netherlands)

    Jansen, GJ; Wildeboer-Veloo, ACM; Tonk, RHJ; Franks, AH; Welling, G

    An automated microscopy-based method using fluorescently labelled 16S rRNA-targeted oligonucleotide probes directed against the predominant groups of intestinal bacteria was developed and validated. The method makes use of the Leica 600HR. image analysis system, a Kodak MegaPlus camera model 1.4 and

  6. Holographic fabrication of 3D photonic crystals through interference of multi-beams with 4 + 1, 5 + 1 and 6 + 1 configurations.

    Science.gov (United States)

    George, D; Lutkenhaus, J; Lowell, D; Moazzezi, M; Adewole, M; Philipose, U; Zhang, H; Poole, Z L; Chen, K P; Lin, Y

    2014-09-22

    In this paper, we are able to fabricate 3D photonic crystals or quasi-crystals through single beam and single optical element based holographic lithography. The reflective optical elements are used to generate multiple side beams with s-polarization and one central beam with circular polarization which in turn are used for interference based holographic lithography without the need of any other bulk optics. These optical elements have been used to fabricate 3D photonic crystals with 4, 5 or 6-fold symmetry. A good agreement has been observed between fabricated holographic structures and simulated interference patterns.

  7. Vibrational imaging and microspectroscopies based on coherent anti-Stokes Raman scattering microscopy

    International Nuclear Information System (INIS)

    Volkmer, Andreas

    2005-01-01

    For noninvasive characterization of chemical species or biological components within a complex heterogeneous system, their intrinsic molecular vibrational properties can be used in contrast mechanisms in optical microscopy. A series of recent advances have made coherent anti-Stokes Raman scattering (CARS) microscopy a powerful technique that allows vibrational imaging with high sensitivity, high spectral resolution and three-dimensional sectioning capability. In this review, we discuss theoretical and experimental aspects of CARS microscopy in a collinear excitation beam geometry. Particular attention is given to the underlying physical principles behind the new features of CARS signal generation under tight focusing conditions. We provide a brief overview of the instrumentation of CARS microscopy and its experimental characterization by means of imaging of model systems and live unstained cells. CARS microscopy offers the possibility of spatially resolved vibrational spectroscopy, providing chemical and physical structure information of molecular specimens on the sub-micrometre length scale. We review multiplex CARS microspectroscopy allowing fast acquisition of frequency-resolved CARS spectra, time-resolved CARS microspectroscopy recording ultrafast Raman free induction decays and CARS correlation spectroscopy probing dynamical processes with chemical selectivity. (topical review)

  8. Fluorescence microscopy.

    Science.gov (United States)

    Sanderson, Michael J; Smith, Ian; Parker, Ian; Bootman, Martin D

    2014-10-01

    Fluorescence microscopy is a major tool with which to monitor cell physiology. Although the concepts of fluorescence and its optical separation using filters remain similar, microscope design varies with the aim of increasing image contrast and spatial resolution. The basics of wide-field microscopy are outlined to emphasize the selection, advantages, and correct use of laser scanning confocal microscopy, two-photon microscopy, scanning disk confocal microscopy, total internal reflection, and super-resolution microscopy. In addition, the principles of how these microscopes form images are reviewed to appreciate their capabilities, limitations, and constraints for operation. © 2014 Cold Spring Harbor Laboratory Press.

  9. Modeling of Fibrin Gels Based on Confocal Microscopy and Light-Scattering Data

    Science.gov (United States)

    Magatti, Davide; Molteni, Matteo; Cardinali, Barbara; Rocco, Mattia; Ferri, Fabio

    2013-01-01

    Fibrin gels are biological networks that play a fundamental role in blood coagulation and other patho/physiological processes, such as thrombosis and cancer. Electron and confocal microscopies show a collection of fibers that are relatively monodisperse in diameter, not uniformly distributed, and connected at nodal points with a branching order of ∼3–4. Although in the confocal images the hydrated fibers appear to be quite straight (mass fractal dimension Dm = 1), for the overall system 1Based on the confocal images, we developed a method to generate three-dimensional (3D) in silico gels made of cylindrical sticks of diameter d, density ρ, and average length 〈L〉, joined at randomly distributed nodal points. The resulting 3D network strikingly resembles real fibrin gels and can be sketched as an assembly of densely packed fractal blobs, i.e., regions of size ξ, where the fiber concentration is higher than average. The blobs are placed at a distance ξ0 between their centers of mass so that they are overlapped by a factor η = ξ/ξ0 and have Dm ∼1.2–1.6. The in silico gels’ structure is quantitatively analyzed by its 3D spatial correlation function g3D(r) and corresponding power spectrum I(q) = FFT3D[g3D(r)], from which ρ, d, Dm, η, and ξ0 can be extracted. In particular, ξ0 provides an excellent estimate of the gel mesh size. The in silico gels’ I(q) compares quite well with real gels’ elastic light-scattering measurements. We then derived an analytical form factor for accurately fitting the scattering data, which allowed us to directly recover the gels’ structural parameters. PMID:23473498

  10. Teaching pathology via online digital microscopy: positive learning outcomes for rurally based medical students.

    Science.gov (United States)

    Sivamalai, Sundram; Murthy, Shashidhar Venkatesh; Gupta, Tarun Sen; Woolley, Torres

    2011-02-01

    Technology has revolutionised teaching. Teaching pathology via digital microscopy (DM) is needed to overcome increasing student numbers, a shortage of pathology academics in regional medical schools, and difficulties with teaching students on rural clinical placement. To identify whether an online DM approach, combining digital pathology software, Web-based slides and classroom management software, delivers effective, practical pathology teaching sessions to medical students located both on campus and on rural placement. An online survey collected feedback from fourth and fifth year undergraduate James Cook University medical students on the importance of 16 listed benefits and challenges of using online DM to teach pathology, via a structured five-point Likert survey. Fifty-three students returned the survey (response rate = 33%). Benefits of online DM to teach pathology rated as 'very important' or 'extremely important' by over 50% of students included: higher quality images; faster learning; more convenient; better technology; everyone sees the same image; greater accessibility; helpful annotations on slides; cost savings; and more opportunity for self-paced learning out-of-hours and for collaborative learning in class. Challenges of online DM rated as 'very important' or 'extremely important' by over 50% of students included: Internet availability in more remote locations and potential problems using online technology during class. Nearly all medical students welcomed learning pathology via online digital technology. DM should improve the quantity, quality, cost and accessibility of pathology teaching by regional medical schools, and has significant implications for the growing emphasis in Australia for decentralised medical education and rural clinical placements. © 2011 The Authors. Australian Journal of Rural Health © National Rural Health Alliance Inc.

  11. Structural properties of silver nanoparticle agglomerates based on transmission electron microscopy: relationship to particle mobility analysis

    International Nuclear Information System (INIS)

    Shin, Weon Gyu; Wang Jing; Mertler, Michael; Sachweh, Bernd; Fissan, Heinz; Pui, David Y. H.

    2009-01-01

    In this work, the structural properties of silver nanoparticle agglomerates generated using condensation and evaporation method in an electric tube furnace followed by a coagulation process are analyzed using Transmission Electron Microscopy (TEM). Agglomerates with mobility diameters of 80, 120, and 150 nm are sampled using the electrostatic method and then imaged by TEM. The primary particle diameter of silver agglomerates was 13.8 nm with a standard deviation of 2.5 nm. We obtained the relationship between the projected area equivalent diameter (d pa ) and the mobility diameter (d m ), i.e., d pa = 0.92 ± 0.03 d m for particles from 80 to 150 nm. We obtained fractal dimensions of silver agglomerates using three different methods: (1) D f = 1.84 ± 0.03, 1.75 ± 0.06, and 1.74 ± 0.03 for d m = 80, 120, and 150 nm, respectively from projected TEM images using a box counting algorithm; (2) fractal dimension (D fL ) = 1.47 based on maximum projected length from projected TEM images using an empirical equation proposed by Koylu et al. (1995) Combust Flame 100:621-633; and (3) mass fractal-like dimension (D fm ) = 1.71 theoretically derived from the mobility analysis proposed by Lall and Friedlander (2006) J Aerosol Sci 37:260-271. We also compared the number of primary particles in agglomerate and found that the number of primary particles obtained from the projected surface area using an empirical equation proposed by Koylu et al. (1995) Combust Flame 100:621-633 is larger than that from using the relationship, d pa = 0.92 ± 0.03 d m or from using the mobility analysis.

  12. Concealed holographic coding for security applications by using a moire technique

    DEFF Research Database (Denmark)

    Zhang, Xiangsu; Dalsgaard, Erik

    1997-01-01

    We present an optical coding technique that enhances the anticounterfeiting power of security holograms. The principles of the technique is based on the moire phenomenon. The code in the hologram has a phase pattern that is invisible and cannot be detected by optical equipment, so that imitation...... is extremely difficult. Holographic, photographic and embossing technique are used in fabricating coded holograms and decoders....

  13. Effect of spatial coherence of LED sources on image resolution in holographic displays

    NARCIS (Netherlands)

    Pourreza Ghoushchi, Vahid; Aas, Mehdi; Ulusoy, Erdem; Ürey, Hakan

    2017-01-01

    Holographic Displays (HDs) provide 3D images with all natural depth cues via computer generated holograms (CGHs) implemented on spatial light modulators (SLMs). HDs are coherent light processing systems based on interference and diffraction, thus they generally use laser light. However, laser

  14. Phases of kinky holographic nuclear matter

    Energy Technology Data Exchange (ETDEWEB)

    Elliot-Ripley, Matthew; Sutcliffe, Paul; Zamaklar, Marija [Department of Mathematical Sciences, Durham University,South Road, Durham (United Kingdom)

    2016-10-17

    Holographic QCD at finite baryon number density and zero temperature is studied within the five-dimensional Sakai-Sugimoto model. We introduce a new approximation that models a smeared crystal of solitonic baryons by assuming spatial homogeneity to obtain an effective kink theory in the holographic direction. The kink theory correctly reproduces a first order phase transition to lightly bound nuclear matter. As the density is further increased the kink splits into a pair of half-kink constituents, providing a concrete realization of the previously suggested dyonic salt phase, where the bulk soliton splits into constituents at high density. The kink model also captures the phenomenon of baryonic popcorn, in which a first order phase transition generates an additional soliton layer in the holographic direction. We find that this popcorn transition takes place at a density below the dyonic salt phase, making the latter energetically unfavourable. However, the kink model predicts only one pop, rather than the sequence of pops suggested by previous approximations. In the kink model the two layers produced by the single pop form the surface of a soliton bag that increases in size as the baryon chemical potential is increased. The interior of the bag is filled with abelian electric potential and the instanton charge density is localized on the surface of the bag. The soliton bag may provide a holographic description of a quarkyonic phase.

  15. Monopole correlations in holographically flavored liquids

    NARCIS (Netherlands)

    Iqbal, N.

    2015-01-01

    Many-body systems with a conserved U(1) current in (2+1) dimensions may be probed by weakly gauging this current and studying correlation functions of magnetic monopole operators in the resulting dynamical gauge theory. We study such monopole correlations in holographic liquids with fundamental

  16. Electronic holographic moire in the micron range

    Science.gov (United States)

    Sciammarella, Cesar A.; Sciammarella, Federico M.

    2001-06-01

    The basic theory behind microscopic electronic holographic moire is presented. Conditions of observation are discussed, and optimal parameters are established. An application is presented as an example where experimental result are statistically analyzed and successfully correlated with an independent method of measurement of the same quantity.

  17. A holographic model for black hole complementarity

    Energy Technology Data Exchange (ETDEWEB)

    Lowe, David A. [Physics Department, Brown University,Providence, RI 02912 (United States); Thorlacius, Larus [University of Iceland, Science Institute,Dunhaga 3, IS-107, Reykjavik (Iceland); The Oskar Klein Centre for Cosmoparticle Physics,Department of Physics, Stockholm University,AlbaNova University Centre, 10691 Stockholm (Sweden)

    2016-12-07

    We explore a version of black hole complementarity, where an approximate semiclassical effective field theory for interior infalling degrees of freedom emerges holographically from an exact evolution of exterior degrees of freedom. The infalling degrees of freedom have a complementary description in terms of outgoing Hawking radiation and must eventually decohere with respect to the exterior Hamiltonian, leading to a breakdown of the semiclassical description for an infaller. Trace distance is used to quantify the difference between the complementary time evolutions, and to define a decoherence time. We propose a dictionary where the evolution with respect to the bulk effective Hamiltonian corresponds to mean field evolution in the holographic theory. In a particular model for the holographic theory, which exhibits fast scrambling, the decoherence time coincides with the scrambling time. The results support the hypothesis that decoherence of the infalling holographic state and disruptive bulk effects near the curvature singularity are complementary descriptions of the same physics, which is an important step toward resolving the black hole information paradox.

  18. Gravitation from entanglement in holographic CFTs

    Energy Technology Data Exchange (ETDEWEB)

    Faulkner, Thomas [Institute for Advanced Study,Princeton, NJ 08540 (United States); Guica, Monica [Department of Physics and Astronomy, University of Pennsylvania,209 S. 33rd St., Philadelphia, PA 19104-6396 (United States); Hartman, Thomas [Kavli Institute for Theoretical Physics, University of California,Santa Barbara, CA 93106-4030 (United States); Myers, Robert C. [Perimeter Institute for Theoretical Physics,31 Caroline Street N., Waterloo, Ontario N2L 2Y5 (Canada); Raamsdonk, Mark Van [Department of Physics and Astronomy, University of British Columbia,6224 Agricultural Road, Vancouver, B.C. V6T 1W9 (Canada)

    2014-03-11

    Entanglement entropy obeys a ‘first law’, an exact quantum generalization of the ordinary first law of thermodynamics. In any CFT with a semiclassical holographic dual, this first law has an interpretation in the dual gravitational theory as a constraint on the spacetimes dual to CFT states. For small perturbations around the CFT vacuum state, we show that the set of such constraints for all ball-shaped spatial regions in the CFT is exactly equivalent to the requirement that the dual geometry satisfy the gravitational equations of motion, linearized about pure AdS. For theories with entanglement entropy computed by the Ryu-Takayanagi formula S=A/(4G{sub N}), we obtain the linearized Einstein equations. For theories in which the vacuum entanglement entropy for a ball is computed by more general Wald functionals, we obtain the linearized equations for the associated higher-curvature theories. Using the first law, we also derive the holographic dictionary for the stress tensor, given the holographic formula for entanglement entropy. This method provides a simple alternative to holographic renormalization for computing the stress tensor expectation value in arbitrary higher derivative gravitational theories.

  19. Holographic entanglement entropy and gravitational anomalies

    NARCIS (Netherlands)

    Castro, A.; Detournay, S.; Iqbal, N.; Perlmutter, E.

    2014-01-01

    We study entanglement entropy in two-dimensional conformal field theories with a gravitational anomaly. In theories with gravity duals, this anomaly is holographically represented by a gravitational Chern-Simons term in the bulk action. We show that the anomaly broadens the Ryu-Takayanagi minimal

  20. Generalized exact holographic mapping with wavelets

    Science.gov (United States)

    Lee, Ching Hua

    2017-12-01

    The idea of renormalization and scale invariance is pervasive across disciplines. It has not only drawn numerous surprising connections between physical systems under the guise of holographic duality, but has also inspired the development of wavelet theory now widely used in signal processing. Synergizing on these two developments, we describe in this paper a generalized exact holographic mapping that maps a generic N -dimensional lattice system to a (N +1 )-dimensional holographic dual, with the emergent dimension representing scale. In previous works, this was achieved via the iterations of the simplest of all unitary mappings, the Haar mapping, which fails to preserve the form of most Hamiltonians. By taking advantage of the full generality of biorthogonal wavelets, our new generalized holographic mapping framework is able to preserve the form of a large class of lattice Hamiltonians. By explicitly separating features that are fundamentally associated with the physical system from those that are basis specific, we also obtain a clearer understanding of how the resultant bulk geometry arises. For instance, the number of nonvanishing moments of the high-pass wavelet filter is revealed to be proportional to the radius of the dual anti-de Sitter space geometry. We conclude by proposing modifications to the mapping for systems with generic Fermi pockets.

  1. On new proposal for holographic BCFT

    Energy Technology Data Exchange (ETDEWEB)

    Chu, Chong-Sun; Miao, Rong-Xin [Department of Physics, National Tsing-Hua University,Hsinchu 30013, Taiwan (China); Physics Division, National Center for Theoretical Sciences,National Tsing-Hua University, Hsinchu 30013, Taiwan (China); Guo, Wu-Zhong [Physics Division, National Center for Theoretical Sciences,National Tsing-Hua University, Hsinchu 30013, Taiwan (China)

    2017-04-14

    This paper is an extended version of our short letter on a new proposal for holographic boundary conformal field, i.e., BCFT. By using the Penrose-Brown-Henneaux (PBH) transformation, we successfully obtain the expected boundary Weyl anomaly. The obtained boundary central charges satisfy naturally a c-like theorem holographically. We then develop an approach of holographic renormalization for BCFT, and reproduce the correct boundary Weyl anomaly. This provides a non-trivial check of our proposal. We also investigate the holographic entanglement entropy of BCFT and find that our proposal gives the expected orthogonal condition that the minimal surface must be normal to the spacetime boundaries if they intersect. This is another support for our proposal. We also find that the entanglement entropy depends on the boundary conditions of BCFT and the distance to the boundary; and that the entanglement wedge behaves a phase transition, which is important for the self-consistency of AdS/BCFT. Finally, we show that the proposal of https://arxiv.org/abs/1105.5165 is too restrictive that it always make vanishing some of the boundary central charges.

  2. Holographic applications of logarithmic conformal field theories

    NARCIS (Netherlands)

    Grumiller, D.; Riedler, W.; Rosseel, J.; Zojer, T.

    2013-01-01

    We review the relations between Jordan cells in various branches of physics, ranging from quantum mechanics to massive gravity theories. Our main focus is on holographic correspondences between critically tuned gravity theories in anti-de Sitter space and logarithmic conformal field theories in

  3. Holographic Lovelock gravities and black holes

    NARCIS (Netherlands)

    de Boer, J.; Kulaxizi, M.; Parnachev, A.

    2010-01-01

    We study holographic implications of Lovelock gravities in AdS spacetimes. For a generic Lovelock gravity in arbitrary spacetime dimensions we formulate the existence condition of asymptotically AdS black holes. We consider small fluctuations around these black holes and determine the constraint on

  4. Scalar Condensation of Holographic Superconductors using ...

    Indian Academy of Sciences (India)

    Abstract. We study holographic superconductors analytically by using the Ginzburg–Landau action with the γ-quartic term | |4. Our results show that γ-term plays a role in the scalar condensation. It is found that the system displays two kinds of critical temperatures. One is independent of γ. But the other increases with ...

  5. Photopolymer for Optical Holography and Holographic Interferometry

    Czech Academy of Sciences Publication Activity Database

    Květoň, M.; Lédl, Vít; Havránek, A.; Fiala, P.

    2010-01-01

    Roč. 295, č. 1 (2010), s. 107-113 ISSN 1022-1360 Institutional research plan: CEZ:AV0Z20430508 Keywords : holographic interferometry * holography * photopolymerization * recording material * refractive index Subject RIV: BH - Optics, Masers, Lasers http://onlinelibrary.wiley.com/doi/10.1002/masy.200900093/pdf

  6. A scanning tunneling microscopy based potentiometry technique and its application to the local sensing of the spin Hall effect

    Directory of Open Access Journals (Sweden)

    Ting Xie

    2017-12-01

    Full Text Available A scanning tunneling microscopy based potentiometry technique for the measurements of the local surface electric potential is presented. A voltage compensation circuit based on this potentiometry technique is developed and employed to maintain a desired tunneling voltage independent of the bias current flow through the film. The application of this potentiometry technique to the local sensing of the spin Hall effect is outlined and some experimental results are reported.

  7. Visualization of magnetic dipolar interaction based on scanning transmission X-ray microscopy

    International Nuclear Information System (INIS)

    Ohtori, Hiroyuki; Iwano, Kaoru; Takeichi, Yasuo; Ono, Kanta; Mitsumata, Chiharu; Yano, Masao; Kato, Akira; Miyamoto, Noritaka; Shoji, Tetsuya; Manabe, Akira

    2014-01-01

    Using scanning transmission X-ray microscopy (STXM), in this report we visualized the magnetic dipolar interactions in nanocrystalline Nd-Fe-B magnets and imaged their magnetization distributions at various applied fields. We calculated the magnetic dipolar interaction by analyzing the interaction between the magnetization at each point and those at the other points on the STXM image.

  8. Structured Illumination-Based Super-Resolution Optical Microscopy for Hemato- and Cyto-Pathology Applications

    Directory of Open Access Journals (Sweden)

    Tieqiao Zhang

    2013-01-01

    Full Text Available Structured illumination fluorescence microscopy utilizes interfering light and the moiré effect to enhance spatial resolution to about a half of that of conventional light microscopy, i.e. approximately 90 nm. In addition to the enhancement in the x and y directions, it also allows enhancement of resolution in the z- direction by the same factor of two (to approximately 220 nm, making it a powerful tool for 3-D morphology studies of fluorescently labeled cells or thin tissue sections. In this report, we applied this technique to several types of blood cells that are commonly seen in hematopathology. Compared with standard brightfield and ordinary fluorescence microscopy images, the 3-D morphology results clearly reveal the morphological features of different types of normal blood cells. We have also used this technique to evaluate morphologies of abnormal erythrocytes and compare them with those recorded on normal cells. The results give a very intuitive presentation of morphological structures of erythrocytes with great details. This research illustrates the potential of this technique to be used in hematology and cyto-pathology studies aimed at identifying nanometer-sized features that cannot be distinguished otherwise with conventional optical microscopy.

  9. Nanoparticle discrimination based on wavelength and lifetime-multiplexed cathodoluminescence microscopy

    NARCIS (Netherlands)

    Garming, Mathijs W H; Weppelman, I Gerward C; de Boer, Pascal; Martínez, Felipe Perona; Schirhagl, Romana; Hoogenboom, Jacob P; Moerland, Robert J

    2017-01-01

    Nanomaterials can be identified in high-resolution electron microscopy images using spectrally-selective cathodoluminescence. Capabilities for multiplex detection can however be limited, e.g., due to spectral overlap or availability of filters. Also, the available photon flux may be limited due to

  10. Volume holographic storage and multiplexing in blends of PMMA and a block methacrylic azopolymer, using 488 nm light pulses in the range of 100 ms to 1 s

    DEFF Research Database (Denmark)

    Forcen, Patricia; Oriol, Luis; Sanchez, Carlos

    2008-01-01

    Blends of polymethylmethacrylate (PMMA) and diblock methacrylic azopolymers have been investigated for holographic storage with short light pulses. Transmission electron microscopy measurements show that the dilution of the block copolymer in PMMA changes the microstructure from a lamellar to a s...

  11. High resolution transmission electron microscopy studies of {sigma} phase in Ni-based single crystal superalloys

    Energy Technology Data Exchange (ETDEWEB)

    Sun Fei [Key Laboratory of Liquid Structure and Heredity of Materials, Ministry of Education, Shandong University, Jinan 250061 (China); Zhang Jianxin, E-mail: jianxin@sdu.edu.cn [Key Laboratory of Liquid Structure and Heredity of Materials, Ministry of Education, Shandong University, Jinan 250061 (China); Liu Pan [Institute of Microstructure and Property of Advanced Materials, Beijing University of Technology, Beijing 100124 (China); Feng Qiang [National Center for Materials Service Safety, University of Science and Technology Beijing, Beijing 100083 (China); State Key Laboratory for Advanced Metals and Materials, University of Science and Technology Beijing, Beijing 100083 (China); Han Xiaodong; Mao Shengcheng [Institute of Microstructure and Property of Advanced Materials, Beijing University of Technology, Beijing 100124 (China)

    2012-09-25

    Graphical abstract: (a) TEM micrograph of {sigma} phase; (b) HRTEM image of {sigma}/{gamma} interface corresponding to the area of the white frame in (a); (c) an enlarged image of area from the white frame in (b). The combination of {sigma}/{gamma} interface appears very well, and a two-atomic-layer step is shown on the {sigma}/{gamma} interface. In addition, {sigma} phase has the orientation relationship of [0 0 1]{sub {gamma}}//[1 1 2{sup Macron }]{sub {sigma}}, (2{sup Macron} 2 0){sub {gamma}}//(1{sup Macron} 1 0){sub {sigma}}, (2{sup Macron }2{sup Macron} 0){sub {gamma}}//(1 1 1){sub {sigma}}; [0 1 1]{sub {gamma}}//[1 1 0]{sub {sigma}}, (1 1{sup Macron} 1){sub {gamma}}//(0 0 1{sup Macron }){sub {sigma}} with the {gamma} phase. Highlights: Black-Right-Pointing-Pointer Elemental characteristic of {sigma} phase is studied by HAADF techniques and EDS analysis. Black-Right-Pointing-Pointer Interfacial characteristics of {sigma}/{gamma} interface are revealed by HRTEM. Black-Right-Pointing-Pointer An atomic structural {sigma}/{gamma} interface with a two-atomic-layer step has been proposed. - Abstract: By means of high resolution transmission electron microscopy (HRTEM) and high-angle annular dark-field image technique (HAADF), morphological of plate-shaped {sigma} phase and interfacial characteristics between plate-shaped {sigma} phase and {gamma} phase in Ni-based single crystal superalloys have been studied. On the basis of HRTEM observations, an atomic structural interface between {sigma} phase and {gamma} phase with a step has been proposed. {sigma} Phase has the relationship of [0 0 1]{sub {gamma}}//[1 1 2{sup Macron }]{sub {sigma}}, (2{sup Macron} 2 0){sub {gamma}}//(1{sup Macron} 1 0){sub {sigma},} (2{sup Macron }2{sup Macron} 0){sub {gamma}}//(1 1 1){sub {sigma}}; [0 1 1]{sub {gamma}}//[1 1 0]{sub {sigma}}, (1 1{sup Macron} 1){sub {gamma}}//(0 0 1{sup Macron }){sub {sigma}} with the {gamma} phase. The compositional characteristics of the {sigma} phase which

  12. Non parametric denoising methods based on wavelets: Application to electron microscopy images in low exposure time

    International Nuclear Information System (INIS)

    Soumia, Sid Ahmed; Messali, Zoubeida; Ouahabi, Abdeldjalil; Trepout, Sylvain; Messaoudi, Cedric; Marco, Sergio

    2015-01-01

    The 3D reconstruction of the Cryo-Transmission Electron Microscopy (Cryo-TEM) and Energy Filtering TEM images (EFTEM) hampered by the noisy nature of these images, so that their alignment becomes so difficult. This noise refers to the collision between the frozen hydrated biological samples and the electrons beam, where the specimen is exposed to the radiation with a high exposure time. This sensitivity to the electrons beam led specialists to obtain the specimen projection images at very low exposure time, which resulting the emergence of a new problem, an extremely low signal-to-noise ratio (SNR). This paper investigates the problem of TEM images denoising when they are acquired at very low exposure time. So, our main objective is to enhance the quality of TEM images to improve the alignment process which will in turn improve the three dimensional tomography reconstructions. We have done multiple tests on special TEM images acquired at different exposure time 0.5s, 0.2s, 0.1s and 1s (i.e. with different values of SNR)) and equipped by Golding beads for helping us in the assessment step. We herein, propose a structure to combine multiple noisy copies of the TEM images. The structure is based on four different denoising methods, to combine the multiple noisy TEM images copies. Namely, the four different methods are Soft, the Hard as Wavelet-Thresholding methods, Bilateral Filter as a non-linear technique able to maintain the edges neatly, and the Bayesian approach in the wavelet domain, in which context modeling is used to estimate the parameter for each coefficient. To ensure getting a high signal-to-noise ratio, we have guaranteed that we are using the appropriate wavelet family at the appropriate level. So we have chosen âĂIJsym8âĂİ wavelet at level 3 as the most appropriate parameter. Whereas, for the bilateral filtering many tests are done in order to determine the proper filter parameters represented by the size of the filter, the range parameter and the

  13. The application of aberration-corrected electron microscopy to the characterization of gold-based catalysts

    Science.gov (United States)

    Herzing, Andrew A.

    Electron microscopy has long been used to study the morphology of heterogeneous catalysts. Recent advances in electron optics now allow for the correction of the inherent spherical aberration (Cs) produced by the objective lens in the scanning transmission electron microscope (STEM, resulting in a significantly improved spatial resolution as well as the ability to use a much larger probe-current than was previously possible. In this thesis, the combination of high-angle annular dark-field (HAADF) imaging and microanalysis by x-ray energy dispersive spectroscopy (XEDS) in an aberration-corrected STEM has been applied for the first time to the characterization of gold-based heterogeneous catalysts. Multi-variate statistical analysis (MSA) has been employed in order to further improve the STEM-XEDS spectrum image data acquired with this technique. In addition, supplemental analysis using electron-energy loss spectroscopy (EELS) and energy-filtered transmission electron microscopy (EFTEM) in an aberration-corrected instrument has also been attempted. These techniques have proven extremely valuable in providing complimentary information to more traditional catalyst characterization techniques such as x-ray photoelectron spectroscopy and x-ray diffraction in four specific problems relating to catalysis. Firstly, the atomic-scale resolution of Cs-corrected HAADF imaging has been utilized to study Au/FeOx catalysts in order to determine the size and structure of the Au clusters present on the support surface. It was discovered that, while both inactive and active catalysts for low-temperature CO oxidation contained large Au particles (> 5 nm) and individual Au atoms, the active catalyst also contained sub-nm clusters comprised of only a few Au atoms. Secondly, novel CeO2 support materials for Au and Au-Pd catalysts were synthesized by precipitation with supercritical CO2. These supports were found to produce significantly more active catalysts than those based on CeO2

  14. Holographic sol-gel monoliths: optical properties and application for humidity sensing

    Science.gov (United States)

    Ilatovskii, Daniil A.; Milichko, Valentin; Vinogradov, Alexander V.; Vinogradov, Vladimir V.

    2018-05-01

    Sol-gel monoliths based on SiO2, TiO2 and ZrO2 with holographic colourful diffraction on their surfaces were obtained via a sol-gel synthesis and soft lithography combined method. The production was carried out without any additional equipment at near room temperature and atmospheric pressure. The accurately replicated wavy structure with nanoscale size of material particles yields holographic effect and its visibility strongly depends on refractive index (RI) of materials. Addition of multi-walled carbon nanotubes (MWCNTs) in systems increases their RI and lends absorbing properties due to extremely high light absorption constant. Further prospective and intriguing applications based on the most successful samples, MWCNTs-doped titania, were investigated as reversible optical humidity sensor. Owing to such property as reversible resuspension of TiO2 nanoparticles while interacting with water, it was proved that holographic xerogels can repeatedly act as humidity sensors. Materials which can be applied as humidity sensors in dependence on holographic response were discovered for the first time.

  15. Holographic sol–gel monoliths: optical properties and application for humidity sensing

    Science.gov (United States)

    Milichko, Valentin; Vinogradov, Alexander V.; Vinogradov, Vladimir V.

    2018-01-01

    Sol–gel monoliths based on SiO2, TiO2 and ZrO2 with holographic colourful diffraction on their surfaces were obtained via a sol–gel synthesis and soft lithography combined method. The production was carried out without any additional equipment at near room temperature and atmospheric pressure. The accurately replicated wavy structure with nanoscale size of material particles yields holographic effect and its visibility strongly depends on refractive index (RI) of materials. Addition of multi-walled carbon nanotubes (MWCNTs) in systems increases their RI and lends absorbing properties due to extremely high light absorption constant. Further prospective and intriguing applications based on the most successful samples, MWCNTs-doped titania, were investigated as reversible optical humidity sensor. Owing to such property as reversible resuspension of TiO2 nanoparticles while interacting with water, it was proved that holographic xerogels can repeatedly act as humidity sensors. Materials which can be applied as humidity sensors in dependence on holographic response were discovered for the first time.

  16. Web-based virtual microscopy at the RWTH Aachen University: didactic concept, methods and analysis of acceptance by the students.

    Science.gov (United States)

    Merk, Magdalene; Knuechel, Ruth; Perez-Bouza, Alberto

    2010-12-20

    Fundamental knowledge of microscopic anatomy and pathology has always been an essential part in medical education. The traditional didactic concept comprises theoretical and practical lessons using a light microscope and glass slides. High-speed Internet connections and technical improvement in whole-slide digital microscopy (commonly termed "virtual microscopy") provide a new and attractive approach for both teachers and students. High picture quality and unlimited temporal and spatial availability of histology samples from different fields are key advantages of web-based digital microscopy. In this report we discuss the technical requirements, system efficiency, optical resolution and didactic concept. Furthermore, we present a review of the experience gained in the course of one year based on an analysis of student acceptance. Three groups with a total of 192 students between the 3rd and 5th year of medical studies attending the practical courses of general and advanced histopathology had access to both glass-mounted and digitalized slides. Prior to exams, students were asked to answer an anonymous questionnaire. The results of the study reflect the high acceptance and intensive use of the web-based digital histology by students, thus encouraging the development of further Web-based learning strategies for the teaching of histology and pathology. 2010 Elsevier GmbH. All rights reserved.

  17. On the Structure of Holographic Polymer-dispersed Polyethylene Glycol

    International Nuclear Information System (INIS)

    Birnkrant, M.; McWilliams, H.; Li, C.; Natarajan, L.; Tondiglia, V.; Sutherland, R.; Lloyd, P.; Bunning, T.

    2006-01-01

    Holographic polymerization (H-P) has been used to fabricate polymer-dispersed liquid crystals and pattern inert nanoparticles. In this article, one-dimensional grating structures of Norland resin and polyethylene glycol (PEG) were achieved using the H-P technique. Both reflection and transmission grating structures were fabricated. The optical properties of the reflection grating structures (also known as Bragg reflectors, BRs) are thermosensitive, which is attributed to the formation and crystallization of PEG crystals. The thermal switching temperature of the BR can be tuned by using different molecular weight PEG samples. The hierarchical structure and morphology of the BR were studied using synchrotron X-ray, polarized light microscopy and transmission electron microscopy. PEG crystals were found to be confined in ∼60 nm thick layers in the BR. Upon crystallization, the PEG lamellae were parallel to the BR surfaces and PEG chains were parallel to the BR normal, resembling the confined crystallization behavior of polyethylene oxide (PEO) in PEO-block-polystyrene (PEO-b-PS) block copolymers. This observation suggests that the tethering effect in the block copolymer systems does not play a major role in PEG chain orientation in the confined nanoenvironment

  18. Experimental transmission electron microscopy studies and phenomenological model of bismuth-based superconducting compounds

    International Nuclear Information System (INIS)

    Elboussiri, Khalid

    1991-01-01

    The main part of this thesis is devoted to an experimental study by transmission electron microscopy of the different phases of the superconducting bismuth cuprates Bi_2Sr_2Ca_n_-_1Cu_nO_2_n_+_4. In high resolution electron microscopy, the two types of incommensurate modulation realized in these compounds have been observed. A model of structure has been proposed from which the simulated images obtained are consistent with observations. The medium resolution images correlated with the electron diffraction data have revealed existence of a multi-soliton regime with latent lock in phases of commensurate periods between 4b and 10b. At last, a description of different phases of these compounds as a result of superstructures from a disordered perovskite type structure is proposed (author) [fr

  19. Electron microscopy study of the deactivation of nickel based catalysts for bio oil hydrodeoxygenation

    DEFF Research Database (Denmark)

    Gardini, Diego; Mortensen, Peter Mølgaard; Carvalho, Hudson W. P.

    2014-01-01

    Hydrodeoxygenation (HDO) is proposed as an efficient way to remove oxygen in bio-oil, improving its quality as a more sustainable alternative to conventional fuels in terms of CO2 neutrality and relative short production cycle [1]. Ni and Ni-MoS2 nanoparticles supported on ZrO2 show potential...... as high-pressure (100 bar) catalysts for purification of bio-oil by HDO. However, the catalysts deactivate in presence of sulfur, chlorine and potassium species, which are all naturally occurring in real bio-oil. The deactivation mechanisms of the Ni/ZrO2 have been investigated through scanning...... transmission electron microscopy (STEM), energy dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM) and X-ray diffraction (XRD). Catalytic testing has been performed using guaiacol in 1-octanol acting as a model compound for bio-oil. Addition of sulphur (0.3 vol% octanethiol) in the feed...

  20. Model-based traction force microscopy reveals differential tension in cellular actin bundles.

    Science.gov (United States)

    Soiné, Jérôme R D; Brand, Christoph A; Stricker, Jonathan; Oakes, Patrick W; Gardel, Margaret L; Schwarz, Ulrich S

    2015-03-01

    Adherent cells use forces at the cell-substrate interface to sense and respond to the physical properties of their environment. These cell forces can be measured with traction force microscopy which inverts the equations of elasticity theory to calculate them from the deformations of soft polymer substrates. We introduce a new type of traction force microscopy that in contrast to traditional methods uses additional image data for cytoskeleton and adhesion structures and a biophysical model to improve the robustness of the inverse procedure and abolishes the need for regularization. We use this method to demonstrate that ventral stress fibers of U2OS-cells are typically under higher mechanical tension than dorsal stress fibers or transverse arcs.

  1. The AdS/CFT Correspondence and Holographic QCD

    International Nuclear Information System (INIS)

    Erlich, J.

    2012-01-01

    Holographic QCD is an extra-dimensional approach to modeling QCD resonances and their interactions. Holographic models encode information about chiral symmetry breaking, Weinberg sum rules, vector meson dominance, and other phenomenological features of QCD. There are two complementary approaches to holographic model building: a top-down approach which begins with string-theory brane configurations, and a bottom-up approach which is more phenomenological. In this talk I will describe the AdS/CFT correspondence, which motivates Holographic QCD, and the techniques used to build holographic models of QCD and to calculate observables in those models. I will also discuss an intriguing light cone approach to Holographic QCD discovered by Brodsky and De Teramond. (author)

  2. A Fast Global Fitting Algorithm for Fluorescence Lifetime Imaging Microscopy Based on Image Segmentation

    OpenAIRE

    Pelet, S.; Previte, M.J.R.; Laiho, L.H.; So, P.T. C.

    2004-01-01

    Global fitting algorithms have been shown to improve effectively the accuracy and precision of the analysis of fluorescence lifetime imaging microscopy data. Global analysis performs better than unconstrained data fitting when prior information exists, such as the spatial invariance of the lifetimes of individual fluorescent species. The highly coupled nature of global analysis often results in a significantly slower convergence of the data fitting algorithm as compared with unconstrained ana...

  3. Challenges of microtome‐based serial block‐face scanning electron microscopy in neuroscience

    Science.gov (United States)

    WANNER, A. A.; KIRSCHMANN, M. A.

    2015-01-01

    Summary Serial block‐face scanning electron microscopy (SBEM) is becoming increasingly popular for a wide range of applications in many disciplines from biology to material sciences. This review focuses on applications for circuit reconstruction in neuroscience, which is one of the major driving forces advancing SBEM. Neuronal circuit reconstruction poses exceptional challenges to volume EM in terms of resolution, field of view, acquisition time and sample preparation. Mapping the connections between neurons in the brain is crucial for understanding information flow and information processing in the brain. However, information on the connectivity between hundreds or even thousands of neurons densely packed in neuronal microcircuits is still largely missing. Volume EM techniques such as serial section TEM, automated tape‐collecting ultramicrotome, focused ion‐beam scanning electron microscopy and SBEM (microtome serial block‐face scanning electron microscopy) are the techniques that provide sufficient resolution to resolve ultrastructural details such as synapses and provides sufficient field of view for dense reconstruction of neuronal circuits. While volume EM techniques are advancing, they are generating large data sets on the terabyte scale that require new image processing workflows and analysis tools. In this review, we present the recent advances in SBEM for circuit reconstruction in neuroscience and an overview of existing image processing and analysis pipelines. PMID:25907464

  4. Diatom Valve Three-Dimensional Representation: A New Imaging Method Based on Combined Microscopies

    Science.gov (United States)

    Ferrara, Maria Antonietta; De Tommasi, Edoardo; Coppola, Giuseppe; De Stefano, Luca; Rea, Ilaria; Dardano, Principia

    2016-01-01

    The frustule of diatoms, unicellular microalgae, shows very interesting photonic features, generally related to its complicated and quasi-periodic micro- and nano-structure. In order to simulate light propagation inside and through this natural structure, it is important to develop three-dimensional (3D) models for synthetic replica with high spatial resolution. In this paper, we present a new method that generates images of microscopic diatoms with high definition, by merging scanning electron microscopy and digital holography microscopy or atomic force microscopy data. Starting from two digital images, both acquired separately with standard characterization procedures, a high spatial resolution (Δz = λ/20, Δx = Δy ≅ 100 nm, at least) 3D model of the object has been generated. Then, the two sets of data have been processed by matrix formalism, using an original mathematical algorithm implemented on a commercially available software. The developed methodology could be also of broad interest in the design and fabrication of micro-opto-electro-mechanical systems. PMID:27690008

  5. Atom-probe field-ion microscopy investigation of CMSX-4 Ni-base superalloy laser beam welds

    International Nuclear Information System (INIS)

    Babu, S.S.; David, S.A.; Vitek, J.M.; Miller, M.K.

    1996-01-01

    CMSX-4 superalloy laser beam welds were investigated by transmission electron microscopy and atom probe field-ion microscopy (APFIM). The weld microstructure consisted of fine (10- to 50-nm) irregularly shaped γ' precipitates (0.65 to 0.75 volume fraction) within the γ matrix. APFIM compositions of the γ and γ' phases were found to be different from those in the base metal. Concentration profiles across the γ and γ' phases showed extensive variations of Cr, Co and Al concentrations as a function of distance within the γ phase. Calculated lattice misfits near the γ/γ' interface in the welds are positive values compared to the negative values for base metal. (orig.)

  6. Development of sensitive holographic devices for physiological metal ion detection

    Science.gov (United States)

    Sabad-e.-Gul; Martin, Suzanne; Cassidy, John; Naydenova, Izabela

    2017-08-01

    The development of selective alkali metal ions sensors in particular is a subject of significant interest. In this respect, the level of blood electrolytes, particularly H+, Na+, K+ and Cl- , is widely used to monitor aberrant physiologies associated with pulmonary emphysema, acute and chronic renal failure, heart failure, diabetes. The sensors reported in this paper are created by holographic recording of surface relief structures in a self-processing photopolymer material. The structures are functionalized by ionophores dibenzo-18-crown-6 (DC) and tetraethyl 4-tert-butylcalix[4]arene (TBC) in plasticised polyvinyl chloride (PVC) matrix. Interrogation of these structures by light allows indirect measurements of chemical analytes' concentration in real time. We present results on the optimisation and testing of the holographic sensor. A self-processing acrylamide-based photopolymer was used to fabricate the required photonic structures. The performance of the sensors for detection of K+ and Na+ was investigated. It was observed that the functionalisation with DC provides a selective response of the devices to K+ over Na+ and TBC coated surface structures are selectively sensitive to Na+. The sensor responds to Na+ within the physiological ranges. Normal levels of Na+ and K+ in human serum lie within the ranges 135-148mM and 3.5-5.3 mM respectively.

  7. Retractions of the gingival margins evaluated by holographic methods

    Science.gov (United States)

    Sinescu, Cosmin; Negrutiu, Meda Lavinia; Manole, Marius; de Sabata, Aldo; Rusu, Laura-Cristina; Stratul, Stefan; Dudea, Diana; Dughir, Ciprian; Duma, Virgil-Florin

    2015-05-01

    The periodontal disease is one of the most common pathological states of the teeth and gums system. The issue is that its evaluation is a subjective one, i.e. it is based on the skills of the dental medical doctor. As for any clinical condition, a quantitative evaluation and monitoring in time of the retraction of the gingival margins is desired. This phenomenon was evaluated in this study with a holographic method by using a He-Ne laser with a power of 13 mW. The holographic system we have utilized - adapted for dentistry applications - is described. Several patients were considered in a comparative study of their state of health - regarding their oral cavity. The impressions of the maxillary dental arch were taken from a patient during his/her first visit and after a period of six months. The hologram of the first model was superposed on the model cast after the second visit. The retractions of the gingival margins could be thus evaluated three-dimensionally in every point of interest. An evaluation of the retraction has thus been made. Conclusions can thus be drawn for the clinical evaluation of the health of the teeth and gums system of each patient.

  8. Layers of deformed instantons in holographic baryonic matter

    Energy Technology Data Exchange (ETDEWEB)

    Preis, Florian [Institut für Theoretische Physik, Technische Universität Wien,1040 Vienna (Austria); Schmitt, Andreas [Mathematical Sciences and STAG Research Centre, University of Southampton,Southampton SO17 1BJ (United Kingdom)

    2016-07-01

    We discuss homogeneous baryonic matter in the decompactified limit of the Sakai-Sugimoto model, improving existing approximations based on flat-space instantons. We allow for an anisotropic deformation of the instantons in the holographic and spatial directions and for a density-dependent distribution of arbitrarily many instanton layers in the bulk. Within our approximation, the baryon onset turns out to be a second-order phase transition, at odds with nature, and there is no transition to quark matter at high densities, at odds with expectations from QCD. This changes when we impose certain constraints on the shape of single instantons, motivated by known features of holographic baryons in the vacuum. Then, a first-order baryon onset and chiral restoration at high density are possible, and at sufficiently large densities two instanton layers are formed dynamically. Our results are a further step towards describing realistic, strongly interacting matter over a large density regime within a single model, desirable for studies of compact stars.

  9. Analysis of higher order harmonics with holographic reflection gratings

    Science.gov (United States)

    Mas-Abellan, P.; Madrigal, R.; Fimia, A.

    2017-05-01

    Silver halide emulsions have been considered one of the most energetic sensitive materials for holographic applications. Nonlinear recording effects on holographic reflection gratings recorded on silver halide emulsions have been studied by different authors obtaining excellent experimental results. In this communication specifically we focused our investigation on the effects of refractive index modulation, trying to get high levels of overmodulation that will produce high order harmonics. We studied the influence of the overmodulation and its effects on the transmission spectra for a wide exposure range by use of 9 μm thickness films of ultrafine grain emulsion BB640, exposed to single collimated beams using a red He-Ne laser (wavelength 632.8 nm) with Denisyuk configuration obtaining a spatial frequency of 4990 l/mm recorded on the emulsion. The experimental results show that high overmodulation levels of refractive index produce second order harmonics with high diffraction efficiency (higher than 75%) and a narrow grating bandwidth (12.5 nm). Results also show that overmodulation produce diffraction spectra deformation of the second order harmonic, transforming the spectrum from sinusoidal to approximation of square shape due to very high overmodulation. Increasing the levels of overmodulation of refractive index, we have obtained higher order harmonics, obtaining third order harmonic with diffraction efficiency (up to 23%) and narrowing grating bandwidth (5 nm). This study is the first step to develop a new easy technique to obtain narrow spectral filters based on the use of high index modulation reflection gratings.

  10. Applying field mapping refractive beam shapers to improve holographic techniques

    Science.gov (United States)

    Laskin, Alexander; Williams, Gavin; McWilliam, Richard; Laskin, Vadim

    2012-03-01

    Performance of various holographic techniques can be essentially improved by homogenizing the intensity profile of the laser beam with using beam shaping optics, for example, the achromatic field mapping refractive beam shapers like πShaper. The operational principle of these devices presumes transformation of laser beam intensity from Gaussian to flattop one with high flatness of output wavefront, saving of beam consistency, providing collimated output beam of low divergence, high transmittance, extended depth of field, negligible residual wave aberration, and achromatic design provides capability to work with several laser sources with different wavelengths simultaneously. Applying of these beam shapers brings serious benefits to the Spatial Light Modulator based techniques like Computer Generated Holography or Dot-Matrix mastering of security holograms since uniform illumination of an SLM allows simplifying mathematical calculations and increasing predictability and reliability of the imaging results. Another example is multicolour Denisyuk holography when the achromatic πShaper provides uniform illumination of a field at various wavelengths simultaneously. This paper will describe some design basics of the field mapping refractive beam shapers and optical layouts of their applying in holographic systems. Examples of real implementations and experimental results will be presented as well.

  11. Human genome sequencing with direct x-ray holographic imaging

    International Nuclear Information System (INIS)

    Rhodes, C.K.

    1993-01-01

    Direct holographic imaging of biological materials is widely applicable to the study of the structure, properties and action of genetic material. This particular application involves the sequencing of the human genome where prospective genomic imaging technology is composed of three subtechnologies, name an x-ray holographic camera, suitable chemistry and enzymology for the preparation of tagged DNA samples, and the illuminator in the form of an x-ray laser. We report appropriate x-ray camera, embodied by the instrument developed by MCR, is available and that suitable chemical and enzymatic procedures exist for the preparation of the necessary tagged DNA strands. Concerning the future development of the x-ray illuminator. We find that a practical small scale x-ray light source is indeed feasible. This outcome requires the use of unconventional physical processes in order to achieve the necessary power-compression in the amplifying medium. The understanding of these new physical mechanisms is developing rapidly. Importantly, although the x-ray source does not currently exist, the understanding of these new physical mechanisms is developing rapidly and the research has established the basic scaling laws that will determine the properties of the x-ray illuminator. When this x-ray source becomes available, an extremely rapid and cost effective instrument for 3-D imaging of biological materials can be applied to a wide range of biological structural assays, including the base-pair sequencing of the human genome and many questions regarding its higher levels of organization

  12. Design of a positioning system for a holographic otoscope

    Science.gov (United States)

    Dobrev, I.; Flores Moreno, J. M.; Furlong, C.; Harrington, E. J.; Rosowski, J. J.; Scarpino, C.

    2010-08-01

    Current ear examination procedures provide mostly qualitative information which results in insufficient or erroneous description of the patient's hearing. Much more quantitative and accurate results can be achieved with a holographic otoscope system currently under development. Various ways of accurate positioning and stabilization of the system in real-life conditions are being investigated by this project in an attempt to bring this new technology to the hospitals and clinics, in order to improve the quality of the treatments and operations of the human ear. The project is focused at developing a mechatronic system capable of positioning the holographic otoscope to the patient's ear and maintaining its relative orientation during the examination. The system will be able to be guided by the examiner, but it will maintain the chosen position automatically. To achieve that, various trajectories are being measured for existing otoscopes being guided by doctors in real medical conditions. Based on that, various kinematic configurations are to be synthesized and their stability and accuracy will be simulated and optimized with FEA. For simplification, the mechanism will contain no actuators, but only adjustable friction elements in a haptic feedback control system. This renders the positioning system safe and easily applicable to current examination rooms. Other means of stabilization of the system are being investigated such as custom designed packaging of all of the otoscope subsystems, interferometrically compensating for the heartbeat induced vibration of the tympanic membrane as well as methods for monitoring and active response to the motion of the patient's head.

  13. Helium ion microscopy based wall thickness and surface roughness analysis of polymer foams obtained from high internal phase emulsion

    International Nuclear Information System (INIS)

    Rodenburg, C.; Viswanathan, P.; Jepson, M.A.E.; Liu, X.; Battaglia, G.

    2014-01-01

    Due to their wide range of applications, porous polymers obtained from high internal phase emulsions have been widely studied using scanning electron microscopy. However, due to their lack of electrical conductivity, quantitative information of wall thicknesses and surface roughness, which are of particular interest to tissue engineering, has not been obtained. Here, Helium Ion Microscopy is used to examine uncoated polymer foams and some very strong but unexpected contrast is observed, the origin of which is established here. Based on this analysis, a method for the measurement of wall thickness variations and wall roughness measurements has been developed, based on the modeling of Helium ion transmission. The results presented here indicate that within the walls of the void structure there exist small features with height variations of ∼30 nm and wall thickness variations from ∼100 nm to larger 340 nm in regions surrounding interconnecting windows within the structure. The suggested imaging method is applicable to other porous carbon based structures with wall thicknesses in the range of 40–340 nm. - Highlights: • The first helium ion microscopy image of uncoated structures formed from HIPEs is presented. • Unusually high contrast features that change with accelerating voltage are observed. • The origin of the observed contrast is determined to be mass thickness contrast. • A new method for quantitative wall thickness variation/roughness measurements is demonstrated

  14. Helium ion microscopy based wall thickness and surface roughness analysis of polymer foams obtained from high internal phase emulsion

    Energy Technology Data Exchange (ETDEWEB)

    Rodenburg, C., E-mail: c.rodenburg@sheffield.ac.uk [Department of Materials Science and Engineering, University of Sheffield, Mappin Street, Sheffield S1 3JD (United Kingdom); Viswanathan, P. [Department of Biomedical Sciences, University of Sheffield, Firth Court, Western Bank Sheffield, Sheffield S10 2 TN (United Kingdom); Jepson, M.A.E. [Department of Materials, Loughborough University, Loughborough, Leicestershire LE11 3TU (United Kingdom); Liu, X. [Carl Zeiss Microscopy GmbH, Carl-Zeiss-Strasse 22, 73447 Oberkochen (Germany); Battaglia, G. [Department of Chemistry University College London, 20 Gordon Street, London WC1H 0AJ (United Kingdom); The MRC/UCL Centre for Medical Molecular Virology, University College London, 20 Gordon Street, London WC1H 0AJ (United Kingdom)

    2014-04-01

    Due to their wide range of applications, porous polymers obtained from high internal phase emulsions have been widely studied using scanning electron microscopy. However, due to their lack of electrical conductivity, quantitative information of wall thicknesses and surface roughness, which are of particular interest to tissue engineering, has not been obtained. Here, Helium Ion Microscopy is used to examine uncoated polymer foams and some very strong but unexpected contrast is observed, the origin of which is established here. Based on this analysis, a method for the measurement of wall thickness variations and wall roughness measurements has been developed, based on the modeling of Helium ion transmission. The results presented here indicate that within the walls of the void structure there exist small features with height variations of ∼30 nm and wall thickness variations from ∼100 nm to larger 340 nm in regions surrounding interconnecting windows within the structure. The suggested imaging method is applicable to other porous carbon based structures with wall thicknesses in the range of 40–340 nm. - Highlights: • The first helium ion microscopy image of uncoated structures formed from HIPEs is presented. • Unusually high contrast features that change with accelerating voltage are observed. • The origin of the observed contrast is determined to be mass thickness contrast. • A new method for quantitative wall thickness variation/roughness measurements is demonstrated.

  15. Higher order corrections to holographic black hole chemistry

    Science.gov (United States)

    Sinamuli, Musema; Mann, Robert B.

    2017-10-01

    We investigate the holographic Smarr relation beyond the large N limit. By making use of the holographic dictionary, we find that the bulk correlates of subleading 1 /N corrections to this relation are related to the couplings in Lovelock gravity theories. We likewise obtain a holographic equation of state and check its validity for a variety of interesting and nontrivial black holes, including rotating planar black holes in Gauss-Bonnet-Born-Infeld gravity, and nonextremal rotating black holes in minimal five-dimensional gauged supergravity. We provide an explanation of the N -dependence of the holographic Smarr relation in terms of contributions due to planar and nonplanar diagrams in the dual theory.

  16. Biophotopol: A Sustainable Photopolymer for Holographic Data Storage Applications

    Directory of Open Access Journals (Sweden)

    Augusto Beléndez

    2012-05-01

    Full Text Available Photopolymers have proved to be useful for different holographic applications such as holographic data storage or holographic optical elements. However, most photopolymers have certain undesirable features, such as the toxicity of some of their components or their low environmental compatibility. For this reason, the Holography and Optical Processing Group at the University of Alicante developed a new dry photopolymer with low toxicity and high thickness called biophotopol, which is very adequate for holographic data storage applications. In this paper we describe our recent studies on biophotopol and the main characteristics of this material.

  17. The compact and inexpensive arrowhead setup for holographic interferometry

    Energy Technology Data Exchange (ETDEWEB)

    Ladera, Celso L; Donoso, Guillermo, E-mail: clladera@usb.v [Departamento de Fisica, Universidad Simon BolIvar, Apdo. 89000, Caracas 1086 (Venezuela, Bolivarian Republic of)

    2011-07-15

    Hologram recording and holographic interferometry are intrinsically sensitive to phase changes, and therefore both are easily perturbed by minuscule optical path perturbations. It is therefore very convenient to bank on holographic setups with a reduced number of optical components. Here we present a compact off-axis holographic setup that requires neither a collimator nor a beam-splitter, and whose layout is reminiscent of an arrowhead. We show that this inexpensive setup is a good alternative for the study and applications of scientific holography by measuring small displacements and deformations of a body. The arrowhead setup will be found particularly useful for holography and holographic interferometry experiments and projects in teaching laboratories.

  18. PREFACE: 6th Workshop on Infrared Spectroscopy and Microscopy with Accelerator-Based Sources (WIRMS11)

    Science.gov (United States)

    Lupi, Stefano; Perucchi, Andrea

    2012-05-01

    This volume of Journal of Physics: Conference Series is dedicated to a subset of papers related to the work presented at the 6th edition of the international Workshop on Infrared Spectroscopy and Microscopy with Accelerator-Based Sources (WIRMS), held in Trieste, Italy, September 4-8 2011. Previous editions of the conference were held in Porquerolles (France), Lake Tahoe (USA), Rathen (Germany), Awaji (Japan), and Banff (Canada). This edition was organized and chaired by Stefano Lupi (Roma La Sapienza) and co-chaired by Andrea Perucchi (Elettra), with the support of the Italian Synchrotron Light Laboratory ELETTRA, which was honored to host the WIRMS workshop in its tenth anniversary. The 6th WIRMS edition addressed several different topics, ranging from biochemistry to strongly correlated materials, from geology to conservation science, and from forensics to the study of cometary dusts. Representatives from the infrared scientific programs at synchrotron light sources and free-electron-laser facilities. This edition was attended by 88 participants, including representatives from the infrared scientific programs at synchrotron light sources and free-electron-laser facilities, who enjoyed the stimulating scientific presentations, several detailed discussions, and the beautiful weather and scenery of the Trieste gulf. Participants came from 16 different nations and four continents, including many young scientists, six of which were supported by the organizers. There were 45 scientific talks divided in 11 sessions: Facilities, Microspectroscopy (I, II, III), Time-Resolved Spectroscopies, Extreme Conditions, Condensed Matter, Near-Field, Imaging, THz Techniques and High-Resolution Spectroscopy. 37 posters were also presented at two very lively evening poster sessions. We would like to use the opportunity of writing this preface to thank all the participants of the workshop for the very high level of their scientific contribution and for the very friendly atmosphere

  19. IR sensitive photorefractive polymers, the first updateable holographic three-dimensional display

    Science.gov (United States)

    Tay, Savas

    This work presents recent advances in the development of infra-red sensitive photorefractive polymers, and updateable near real-time holographic 3D displays based on photorefractive polymers. Theoretical and experimental techniques used for design, fabrication and characterization of photorefractive polymers are outlined. Materials development and technical advances that made possible the use of photorefractive polymers for infra-red free-space optical communications, and 3D holographic displays are presented. Photorefractive polymers are dynamic holographic materials that allow recording of highly efficient reversible holograms. The longest operation wavelength for a photorefractive polymer before this study has been 950nm, far shorter than 1550nm, the wavelength of choice for optical communications and medical imaging. The polymers shown here were sensitized using two-photon absorption, a third order nonlinear effect, beyond the linear absorption spectrum of organic dyes, and reach 40% diffraction efficiency with a 35ms response time at this wavelength. As a consequence of two-photon absorption sensitization they exhibit non-destructive readout, which is an important advantage for applications that require high signal-to-noise ratios. Holographic 3D displays provide highly realistic images without the need for special eyewear, making them valuable tools for applications that require "situational awareness" such as medical, industrial and military imaging. Current commercially available holographic 3D displays employ photopolymers that lack image updating capability, resulting in their restricted use and high cost per 3D image. The holographic 3D display shown here employs photorefractive polymers with nearly 100% diffraction efficiency and fast writing time, hours of image persistence, rapid erasure and large area, a combination of properties that has not been shown before. The 3D display is based on stereography and utilizes world's largest photorefractive

  20. Monolithically Integrated, Mechanically Resilient Carbon-Based Probes for Scanning Probe Microscopy

    Science.gov (United States)

    Kaul, Anupama B.; Megerian, Krikor G.; Jennings, Andrew T.; Greer, Julia R.

    2010-01-01

    Scanning probe microscopy (SPM) is an important tool for performing measurements at the nanoscale in imaging bacteria or proteins in biology, as well as in the electronics industry. An essential element of SPM is a sharp, stable tip that possesses a small radius of curvature to enhance spatial resolution. Existing techniques for forming such tips are not ideal. High-aspect-ratio, monolithically integrated, as-grown carbon nanofibers (CNFs) have been formed that show promise for SPM applications by overcoming the limitations present in wet chemical and separate substrate etching processes.

  1. Polythiophenes and fullerene derivatives based donor-acceptor system: topography by atomic force microscopy

    International Nuclear Information System (INIS)

    Marcakova, M. L.; Repovsky, D.; Cik, G.; Velic, D.

    2017-01-01

    The goal of this work is to examine the surface of a polythiophene/fullerene film in order to understand the structure. In this work polythiophene is used as electron donor and fullerene-derivative is used as electron acceptor. Atomic force microscopy (AFM), is an ideal method to study surfaces and nanostructures. Surfaces of fullerene C60 , fullerene-derivates PCBM, polythiophene P12 and a mixture of P12 and PCBM are characterized. In all samples, the average roughness, the arithmetical value of divergence from the high of the surface, is determined concluding that P12 and PCBM mix together well and form a film with specific topography. (authors)

  2. Colocalization coefficients evaluating the distribution of molecular targets in microscopy methods based on pointed patterns

    Czech Academy of Sciences Publication Activity Database

    Pastorek, Lukáš; Sobol, Margaryta; Hozák, Pavel

    2016-01-01

    Roč. 146, č. 4 (2016), s. 391-406 ISSN 0948-6143 R&D Projects: GA TA ČR(CZ) TE01020118; GA ČR GA15-08738S; GA MŠk(CZ) ED1.1.00/02.0109; GA MŠk(CZ) LM2015062 Grant - others:Human Frontier Science Program(FR) RGP0017/2013 Institutional support: RVO:68378050 Keywords : Colocalization * Quantitative analysis * Pointed patterns * Transmission electron microscopy * Manders' coefficients * Immunohistochemistry Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.553, year: 2016

  3. Holographic associative memories in document retrieval systems

    International Nuclear Information System (INIS)

    Becker, P.J.; Bolle, H.; Keller, A.; Kistner, W.; Riecke, W.D.; Wagner, U.

    1979-03-01

    The objective of this work was the implementation of a holographic memory with associative readout for a document retrieval system. Taking advantage of the favourable properties of holography - associative readout of the memory, parallel processing in the response store - may give shorter response times than sequentially organized data memories. Such a system may also operate in the interactive mode including chain associations. In order to avoid technological difficulties, the experimental setup made use of commercially available components only. As a result an improved holographic structure is proposed which uses volume holograms in photorefractive crystals as storage device. In two chapters of appendix we give a review of the state of the art of electrooptic devices for coherent optical data processing and of competing technologies (semiconductor associative memories and associative program systems). (orig.) [de

  4. Holographic mutual information of two disjoint spheres

    Science.gov (United States)

    Chen, Bin; Fan, Zhong-Ying; Li, Wen-Ming; Zhang, Cheng-Yong

    2018-04-01

    We study quantum corrections to holographic mutual information for two disjoint spheres at a large separation by using the operator product expansion of the twist field. In the large separation limit, the holographic mutual information is vanishing at the semiclassical order, but receive quantum corrections from the fluctuations. We show that the leading contributions from the quantum fluctuations take universal forms as suggested from the boundary CFT. We find the universal behavior for the scalar, the vector, the tensor and the fermionic fields by treating these fields as free fields propagating in the fixed background and by using the 1 /n prescription. In particular, for the fields with gauge symmetries, including the massless vector boson and massless graviton, we find that the gauge parts in the propagators play an indispensable role in reading the leading order corrections to the bulk mutual information.

  5. Properties of multilayer nonuniform holographic structures

    International Nuclear Information System (INIS)

    Pen, E F; Rodionov, Mikhail Yu

    2010-01-01

    Experimental results and analysis of properties of multilayer nonuniform holographic structures formed in photopolymer materials are presented. The theoretical hypotheses is proved that the characteristics of angular selectivity for the considered structures have a set of local maxima, whose number and width are determined by the thicknesses of intermediate layers and deep holograms and that the envelope of the maxima coincides with the selectivity contour of a single holographic array. It is also experimentally shown that hologram nonuniformities substantially distort shapes of selectivity characteristics: they become asymmetric, the local maxima differ in size and the depths of local minima reduce. The modelling results are made similar to experimental data by appropriately choosing the nonuniformity parameters. (imaging and image processing. holography)

  6. Reheating of the Universe as holographic thermalization

    Energy Technology Data Exchange (ETDEWEB)

    Kawai, Shinsuke, E-mail: shinsuke.kawai@gmail.com [Department of Physics, Sungkyunkwan University, Suwon 16419 (Korea, Republic of); Nakayama, Yu [California Institute of Technology, 452-48, Pasadena, CA 91125 (United States); Kavli Institute for the Physics and Mathematics of the Universe (WPI), Todai Institutes for Advanced Study, Kashiwa, Chiba 277-8583 (Japan)

    2016-08-10

    Assuming gauge/gravity correspondence we study reheating of the Universe using its holographic dual. Inflaton decay and thermalisation of the decay products correspond to collapse of a spherical shell and formation of a blackhole in the dual anti-de Sitter (AdS) spacetime. The reheating temperature is computed as the Hawking temperature of the developed blackhole probed by a dynamical boundary, and is determined by the inflaton energy density and the AdS radius, with corrections from the dynamics of the shell collapse. For given initial energy density of the inflaton field the holographic model typically gives lower reheating temperature than the instant reheating scenario, while it is shown to be safely within phenomenological bounds.

  7. Reheating of the Universe as holographic thermalization

    Directory of Open Access Journals (Sweden)

    Shinsuke Kawai

    2016-08-01

    Full Text Available Assuming gauge/gravity correspondence we study reheating of the Universe using its holographic dual. Inflaton decay and thermalisation of the decay products correspond to collapse of a spherical shell and formation of a blackhole in the dual anti-de Sitter (AdS spacetime. The reheating temperature is computed as the Hawking temperature of the developed blackhole probed by a dynamical boundary, and is determined by the inflaton energy density and the AdS radius, with corrections from the dynamics of the shell collapse. For given initial energy density of the inflaton field the holographic model typically gives lower reheating temperature than the instant reheating scenario, while it is shown to be safely within phenomenological bounds.

  8. Holographic entanglement for Chern-Simons terms

    International Nuclear Information System (INIS)

    Azeyanagi, Tatsuo; Loganayagam, R.; Ng, Gim Seng

    2017-01-01

    We derive the holographic entanglement entropy contribution from pure and mixed gravitational Chern-Simons(CS) terms in AdS 2k+1 . This is done through two different methods: first, by a direct evaluation of CS action in a holographic replica geometry and second by a descent of Dong’s derivation applied to the corresponding anomaly polynomial. In lower dimensions (k=1,2), the formula coincides with the Tachikawa formula for black hole entropy from gravitational CS terms. New extrinsic curvature corrections appear for k≥3: we give explicit and concise expressions for the two pure gravitational CS terms in AdS 7 and present various consistency checks, including agreements with the black hole entropy formula when evaluated at the bifurcation surface.

  9. Holographic entanglement for Chern-Simons terms

    Energy Technology Data Exchange (ETDEWEB)

    Azeyanagi, Tatsuo [Département de Physique, Ecole Normale Supérieure, CNRS,24 rue Lhomond, 75005 Paris (France); Loganayagam, R. [School of Natural Sciences, Institute for Advanced Study,Princeton, NJ 08540 (United States); Ng, Gim Seng [Department of Physics, McGill University,Montréal, QC H3A 2T8 (Canada)

    2017-02-01

    We derive the holographic entanglement entropy contribution from pure and mixed gravitational Chern-Simons(CS) terms in AdS{sub 2k+1}. This is done through two different methods: first, by a direct evaluation of CS action in a holographic replica geometry and second by a descent of Dong’s derivation applied to the corresponding anomaly polynomial. In lower dimensions (k=1,2), the formula coincides with the Tachikawa formula for black hole entropy from gravitational CS terms. New extrinsic curvature corrections appear for k≥3: we give explicit and concise expressions for the two pure gravitational CS terms in AdS{sub 7} and present various consistency checks, including agreements with the black hole entropy formula when evaluated at the bifurcation surface.

  10. Holographic entanglement for Chern-Simons terms

    Science.gov (United States)

    Azeyanagi, Tatsuo; Loganayagam, R.; Ng, Gim Seng

    2017-02-01

    We derive the holographic entanglement entropy contribution from pure and mixed gravitational Chern-Simons(CS) terms in AdS2 k+1. This is done through two different methods: first, by a direct evaluation of CS action in a holographic replica geometry and second by a descent of Dong's derivation applied to the corresponding anomaly polynomial. In lower dimensions ( k = 1 , 2), the formula coincides with the Tachikawa formula for black hole entropy from gravitational CS terms. New extrinsic curvature corrections appear for k ≥ 3: we give explicit and concise expressions for the two pure gravitational CS terms in AdS7 and present various consistency checks, including agreements with the black hole entropy formula when evaluated at the bifurcation surface.

  11. Mapping of Proteomic Composition on the Surfaces of Bacillus spores by Atomic Force Microscopy-based Immunolabeling

    Energy Technology Data Exchange (ETDEWEB)

    Plomp, M; Malkin, A J

    2008-06-02

    Atomic force microscopy provides a unique capability to image high-resolution architecture and structural dynamics of pathogens (e.g. viruses, bacteria and bacterial spores) at near molecular resolution in native conditions. Further development of atomic force microscopy in order to enable the correlation of pathogen protein surface structures with specific gene products is essential to understand the mechanisms of the pathogen life cycle. We have applied an AFM-based immunolabeling technique for the proteomic mapping of macromolecular structures through the visualization of the binding of antibodies, conjugated with nanogold particles, to specific epitopes on Bacillus spore surfaces. This information is generated while simultaneously acquiring the surface morphology of the pathogen. The immunospecificity of this labeling method was established through the utilization of specific polyclonal and monoclonal antibodies that target spore coat and exosporium epitopes of Bacillus atrophaeus and Bacillus anthracis spores.

  12. 3D imaging of cement-based materials at submicron resolution by combining laser scanning confocal microscopy with serial sectioning.

    Science.gov (United States)

    Yio, M H N; Mac, M J; Wong, H S; Buenfeld, N R

    2015-05-01

    In this paper, we present a new method to reconstruct large volumes of nontransparent porous materials at submicron resolution. The proposed method combines fluorescence laser scanning confocal microscopy with serial sectioning to produce a series of overlapping confocal z-stacks, which are then aligned and stitched based on phase correlation. The method can be extended in the XY plane to further increase the overall image volume. Resolution of the reconstructed image volume does not degrade with increase in sample size. We have used the method to image cementitious materials, hardened cement paste and concrete and the results obtained show that the method is reliable. Possible applications of the method such as three-dimensional characterization of the pores and microcracks in hardened concrete, three-dimensional particle shape characterization of cementitious materials and three-dimensional characterization of other porous materials such as rocks and bioceramics are discussed. © 2015 The Authors Journal of Microscopy © 2015 Royal Microscopical Society.

  13. Microscopy based studies on the interaction of bio-based silver nanoparticles with Bombyx mori Nuclear Polyhedrosis virus.

    Science.gov (United States)

    Tamilselvan, Selvaraj; Ashokkumar, Thirunavukkarasu; Govindaraju, Kasivelu

    2017-04-01

    In the present investigation, silver nanoparticles (AgNPs) interactions with Bombyx mori Nuclear Polyhedrosis virus (BmNPV) were characterized using High-Resolution Scanning Electron Microscopy (HR-SEM), Energy Dispersive X-ray Analysis (EDAX), Transmission Electron Microscopy (TEM), Atomic Force Microcopy (AFM) and Confocal Microscope (CM). HR-SEM study reveals that the biosynthesized AgNPs have interacted with BmNPV and were found on the surface. TEM micrographs of normal and viral polyhedra treated with AgNPs showed that the nanoparticles were accumulated in the membrane and it was noted that some of the AgNPs successfully penetrated the membrane by reaching the capsid of BmNPV. AFM and confocal microscopy studies reveal that the disruption in the shell membrane tends to lose its stability due to exposure of AgNPs to BmNPV. Copyright © 2017 Elsevier B.V. All rights reserved.

  14. Interacting holographic dark energy with logarithmic correction

    OpenAIRE

    Jamil, Mubasher; Farooq, M. Umar

    2010-01-01

    The holographic dark energy (HDE) is considered to be the most promising candidate of dark energy. Its definition is originally motivated from the entropy-area relation which depends on the theory of gravity under consideration. Recently a new definition of HDE is proposed with the help of quantum corrections to the entropy-area relation in the setup of loop quantum cosmology. Using this new definition, we investigate the model of interacting dark energy and derive its effective equation of s...

  15. Magnonic holographic imaging of magnetic microstructures

    Energy Technology Data Exchange (ETDEWEB)

    Gutierrez, D.; Chiang, H.; Bhowmick, T.; Volodchenkov, A.D.; Ranjbar, M.; Liu, G.; Jiang, C.; Warren, C. [Department of Electrical and Computer Engineering, University of California - Riverside, Riverside, CA 92521 (United States); Khivintsev, Y.; Filimonov, Y. [Kotelnikov Institute of Radioengineering and Electronics of Russian Academy of Sciences, Saratov Branch, Saratov 410019 (Russian Federation); Saratov State University, Saratov 410012 (Russian Federation); Garay, J.; Lake, R.; Balandin, A.A. [Department of Electrical and Computer Engineering, University of California - Riverside, Riverside, CA 92521 (United States); Khitun, A., E-mail: akhitun@engr.ucr.edu [Department of Electrical and Computer Engineering, University of California - Riverside, Riverside, CA 92521 (United States)

    2017-04-15

    We propose and demonstrate a technique for magnetic microstructure imaging via their interaction with propagating spin waves. In this approach, the object of interest is placed on top of a magnetic testbed made of material with low spin wave damping. There are micro-antennas incorporated in the testbed. Two of these antennas are used for spin wave excitation while another one is used for the detecting of inductive voltage produced by the interfering spin waves. The measurements are repeated for different phase differences between the spin wave generating antennas which is equivalent to changing the angle of illumination. The collected data appear as a 3D plot – the holographic image of the object. We present experimental data showing magnonic holographic images of a low-coercivity Si/Co sample, a high-coercivity sample made of SrFe{sub 12}O{sub 19} and a diamagnetic copper sample. We also present images of the three samples consisting of a different amount of SrFe{sub 12}O{sub 19} powder. The imaging was accomplished on a Y{sub 3}Fe{sub 2}(FeO{sub 4}){sub 3} testbed at room temperature. The obtained data reveal the unique magnonic signatures of the objects. Experimental data is complemented by the results of numerical modeling, which qualitatively explain the characteristic features of the images. Potentially, magnonic holographic imaging may complement existing techniques and be utilized for non-destructive in-situ magnetic object characterization. The fundamental physical limits of this approach are also discussed. - Highlights: • A technique for magnetic microstructure imaging via their interaction with propagating spin waves is proposed. • In this technique, magnetic structures appear as 3D objects. • Several holographic images of magnetic microstructures are presented.

  16. Holographic nondestructive testing in bone biomechanics

    Science.gov (United States)

    Silvennoinen, Raimo V. J.; Nygren, Kaarlo; Karna, Markku

    1992-08-01

    Holographic nondestructive testing (HNDT) is used to investigate the complex structures of bones of various shapes and sizes subjected to forces. During the course of the present study three antlered deer skulls of different species were investigated, and significant species- specific differences were observed. The HNDT method was also used to verify the advanced healing of an osteosynthetized sheep jawbone. Radioulnar bones of normal and orphaned moose calves were subjected to the bending test. Different bending dynamics were observed.

  17. Holographic Location of Distant Points (PREPRINT)

    Science.gov (United States)

    2010-06-01

    respects and the nonimaging systems have significant advantages. This paper shows how to use holograms to construct a flat, solid, small, accurate, small... nonimaging point location system. 15. SUBJECT TERMS imagery, holographic 16. SECURITY CLASSIFICATION OF: 17. LIMITATION OF ABSTRACT: SAR 18...respects and the nonimaging systems we have discussed earlier (1, 2) have significant advantages. This paper shows how to use holograms to construct a

  18. Holographic processing of track chamber data

    Energy Technology Data Exchange (ETDEWEB)

    Bykovsky, Y A; Larkin, A I; Markilov, A A; Starikov, S N [Moskovskij Fiziko-Tekhnicheskij Inst. (USSR)

    1975-12-01

    The holographic pattern recognition method was applied for processing of track chamber photographs. Experiments on detection of such events as a definitely directed track, an angle formed by two tracks, a three-pronged star, a definitely curved track were performed by using models. It is proposed to recognize these events in a filmshot by the shape of correlation signals. The experiment to recognize the event in a real bubble chamber filmshot was realized; requirements to the processing films were determined.

  19. Holographic cosmological models on the braneworld

    Energy Technology Data Exchange (ETDEWEB)

    Lepe, Samuel [Instituto de Fisica, Pontificia Universidad Catolica de Valparaiso, Casilla 4950, Valparaiso (Chile); Saavedra, Joel [Instituto de Fisica, Pontificia Universidad Catolica de Valparaiso, Casilla 4950, Valparaiso (Chile)], E-mail: joel.saavedra@ucv.cl; Pena, Francisco [Departamento de Ciencias Fisicas, Facultad de Ingenieria, Ciencias y Administracion, Universidad de la Frontera, Avda. Francisco Salazar 01145, Casilla 54-D, Temuco (Chile)

    2009-01-26

    In this Letter we have studied a closed universe which a holographic energy on the brane whose energy density is described by {rho}(H)=3c{sup 2}H{sup 2} and we obtain an equation for the Hubble parameter. This equation gave us different physical behavior depending if c{sup 2}>1 or c{sup 2}<1 against of the sign of the brane tension.

  20. Holographic Superconductivity with Gauss-Bonnet gravity

    OpenAIRE

    Gregory, Ruth

    2010-01-01

    I review recent work on holographic superconductivity with Einstein-Gauss-Bonnet gravity, and show how the critical temperature of the superconductor depends on both gravitational backreaction and the Gauss-Bonnet parameter, using both analytic and numerical arguments. I also review computations of the conductivity, finding the energy gap, and demonstrating that there is no universal gap ratio, $\\omega_g/T_c$, for these superconductors.