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Relative cytotoxicity of complexes of platinum(II and palladium(II against pure cell culture Paramecium caudatum and human cell lines A431 and HaCaT

Directory of Open Access Journals (Sweden)

Aleksei Vladimirovich Eremin

2018-04-01

Full Text Available The results of cytotoxicity cisplatin-like complexes of platinum(II and palladium(II are presented. The cytotoxicity was researched by method of biotesting with Paramecium caudatum and by MTT-assay with human cells: epidermoid carcimoma A431 and minimal transformed aneuploid keratinocytes HaCaT. Cytotoxicity of complexes toward protists is high, however, comparatively HaCaT are more sensitive than A431. Furthemore, cytotoxicity of palladium(II complexes is higher than the analogues with platinum(II.
HVOF-Sprayed Nano TiO2-HA Coatings Exhibiting Enhanced Biocompatibility

Science.gov (United States)

Lima, R. S.; Dimitrievska, S.; Bureau, M. N.; Marple, B. R.; Petit, A.; Mwale, F.; Antoniou, J.

2010-01-01

Biomedical thermal spray coatings produced via high-velocity oxy-fuel (HVOF) from nanostructured titania (n-TiO2) and 10 wt.% hydroxyapatite (HA) (n-TiO2-10wt.%HA) powders have been engineered as possible future alternatives to HA coatings deposited via air plasma spray (APS). This approach was chosen due to (i) the stability of TiO2 in the human body (i.e., no dissolution) and (ii) bond strength values on Ti-6Al-4V substrates more than two times higher than those of APS HA coatings. To explore the bioperformance of these novel materials and coatings, human mesenchymal stem cells (hMSCs) were cultured from 1 to 21 days on the surface of HVOF-sprayed n-TiO2 and n-TiO2-10 wt.%HA coatings. APS HA coatings and uncoated Ti-6Al-4V substrates were employed as controls. The profiles of the hMSCs were evaluated for (i) cellular proliferation, (ii) biochemical analysis of alkaline phosphatase (ALP) activity, (iii) cytoskeleton organization (fluorescent/confocal microscopy), and (iv) cell/substrate interaction via scanning electron microscopy (SEM). The biochemical analysis indicated that the hMSCs cultured on n-TiO2-10 wt.%HA coatings exhibited superior levels of bioactivity than hMSCs cultured on APS HA and pure n-TiO2 coatings. The cytoskeleton organization demonstrated a higher degree of cellular proliferation on the HVOF-sprayed n-TiO2-10wt.%HA coatings when compared to the control coatings. These results are considered promising for engineering improved performance in the next generation of thermally sprayed biomedical coatings.
A high resolution atlas of Mg II profiles

Science.gov (United States)

Ewald, R.; Nichols-Bohlin, Joy Y.; Kondo, Yoji

1990-01-01

An atlas of high dispersion Mg II profiles for standard stars of spectral types B0 through G9 is presented. The atlas contains plots of the Mg II profiles for approximately 65 stars and associated equivalent width measurements for both absorption and emission components, and the subordinate lines. The atlas is used to investigate systematic behavior of the Mg II profiles and correlation of the behavior with spectral classification.
The novel kinetics expression of Cadmium (II) removal using green adsorbent horse dung humic acid (Hd-Ha)

Science.gov (United States)

Basuki, Rahmat; Santosa, Sri Juari; Rusdiarso, Bambang

2017-03-01

Humic acid from dry horse dung powder has been prepared and this horse dung humic acid (HD-HA) was then applied as a sorbent to adsorb Cadmium(II) from a solution. Characterization of HD-HA was conducted by detection of its functional group, UV-Vis spectra, ash level, and total acidity. Result of the work showed that HD-HA had similar character compared with peat soil humic acid (PS-HA) and previous researchers. The adsorption study of this work was investigated by batch experiment in pH 5. The thermodynamics parameters in this work were determined by the Langmuir isotherm model for monolayer sorption and Freundlich isotherm model multilayer sorption. Monolayer sorption capacity (b) for HD-HA was 1.329 × 10-3 mol g-1, equilibrium constant (K) was 5.651 (mol/L)-1, and multilayer sorption capacity was 2.646 × 10-2 mol g-1. The kinetics parameters investigated in this work were determined by the novel kinetics expression resulted from the mathematical derivation the availability of binding sites of sorbent. Adsorption rate constant (ka) from this novel expression was 43.178 min-1 (mol/L)-1 and desorption rate constant (kd) was 1.250 × 10-2 min-1. Application of the kinetics model on sorption Cd(II) onto HD-HA showed the nearly all of models gave a good linearity. However, only this proposed kinetics expression has good relation with Langmuir model. The novel kinetics expression proposed in this paper seems to be more realistic and reasonable and close to the experimental real condition because the value of ka/kd (3452 (mol/L)-1) was fairly close with K from Langmuir isotherm model (5651 (mol/L)-1). Comparison of this novel kinetics expression with well-known Lagergren pseudo-first order kinetics and Ho pseudo-second order kinetics was also critically discussed in this paper.
HaCaT Keratinocytes and Primary Epidermal Keratinocytes Have Different Transcriptional Profiles of Cornified Envelope-Associated Genes to T Helper Cell Cytokines

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Seo, Min-Duk; Kang, Tae Jin; Lee, Chang Hoon; Lee, Ai-Young; Noh, Minsoo

2012-01-01

HaCaT cells are the immortalized human keratinocytes and have been extensively used to study the epidermal homeostasis and its pathophysiology. T helper cells play a role in various chronic dermatological conditions and they can affect skin barrier homeostasis. To evaluate whether HaCaT cells can be used as a model cell system to study abnormal skin barrier development in various dermatologic diseases, we analyzed the gene expression profile of epidermal differentiation markers of HaCaT cells in response to major T helper (Th) cell cytokines, such as IFNγ, IL-4, IL-17A and IL-22. The gene transcriptional profile of cornified envelope-associated proteins, such as filaggrin, loricrin, involucrin and keratin 10 (KRT10), in HaCaT cells was generally different from that in normal human keratinocytes (NHKs). This suggests that HaCaT cells have a limitation as a model system to study the pathophysiological mechanism associated with the Th cell cytokine-dependent changes in cornified envelope-associated proteins which are essential for normal skin barrier development. In contrast, the gene transcription profile change of human β2-defensin (HBD2) in response to IFNγ, IL-4 or IL-17A in HaCaT cells was consistent with the expression pattern of NHKs. IFNγ also up-regulated transglutaminase 2 (TGM2) gene transcription in both HaCaT cells and NHKs. As an alternative cell culture system for NHKs, HaCaT cells can be used to study molecular mechanisms associated with abnormal HBD2 and TGM2 expression in response to IFNγ, IL-4 or IL-17A. PMID:24116291
Point mutation in activated c-Ha-ras gene of a chemically induced transplantable human pancreas carcinoma

International Nuclear Information System (INIS)

Maheshwari, K.K.; Parsa, I.

1986-01-01

The authors have reported a model of human pancreas carcinogenesis where repeated treatment with MNU of explants results in the development of transplantable carcinoma. This report compares the endonuclease digests of DNAs from normal human pancreas (HP) and MNU-induced transplantable tumor (HP-T1) analyzed with 32 P-labelled Ha-ras probe prepared from clone BS-9. The hybridization patterns of BamHI, BglII, EcoRI and HindIII digests of HP were significantly different from those of HP-T1. In EcoRI digests a 3.0 kb fragments of HP-T1 DNA hybridized with Ha-ras probe instead of a 4.3 kb fragments seen in HP DNA. The pattern for HindIII digests was similar to those of EcoRI. The BgIII digests of HP DNA revealed two hybridizing fragments of 8.0 and 4.3 kb whereas those of HP-T1 DNA fragments measured 8.5 and 4.0 kb. BamHI treated HP DNA showed only hybridizing fragments of 6.6 kb while the HP-T1 DNA showed to hybridizing fragments of 6.8 and 7.2 kb. The digested DNAs by HhaI, HinfI, KpnI, pstI, PvuII, SaII, SstI, TaqI and XbaI showed similar hybridization profiles. The point mutation in c-Ha-ras was examined in the HpaII and MspI double digests of both DNAs by 0.6 Kb SmaI fragments of pEJ. The hybridized fragments measured 412 and 355 bp in DNA digests from tumor and normal pancreas respectively
SURFACE BRIGHTNESS PROFILES OF DWARF GALAXIES. II. COLOR TRENDS AND MASS PROFILES

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Herrmann, Kimberly A. [Penn State Mont Alto, 1 Campus Drive, Mont Alto, PA 17237 (United States); Hunter, Deidre A. [Lowell Observatory, 1400 West Mars Hill Road, Flagstaff, AZ 86001 (United States); Elmegreen, Bruce G., E-mail: kah259@psu.edu, E-mail: dah@lowell.edu, E-mail: bge@us.ibm.com [IBM T. J. Watson Research Center, 1101 Kitchawan Road, Yorktown Heights, NY 10598 (United States)

2016-06-01

In this second paper of a series, we explore the B − V , U − B , and FUV−NUV radial color trends from a multi-wavelength sample of 141 dwarf disk galaxies. Like spirals, dwarf galaxies have three types of radial surface brightness profiles: (I) single exponential throughout the observed extent (the minority), (II) down-bending (the majority), and (III) up-bending. We find that the colors of (1) Type I dwarfs generally become redder with increasing radius, unlike spirals which have a blueing trend that flattens beyond ∼1.5 disk scale lengths, (2) Type II dwarfs come in six different “flavors,” one of which mimics the “U” shape of spirals, and (3) Type III dwarfs have a stretched “S” shape where the central colors are flattish, become steeply redder toward the surface brightness break, then remain roughly constant beyond, which is similar to spiral Type III color profiles, but without the central outward bluing. Faint (−9 > M{sub B} > −14) Type II dwarfs tend to have continuously red or “U” shaped colors and steeper color slopes than bright (−14 > M{sub B} > −19) Type II dwarfs, which additionally have colors that become bluer or remain constant with increasing radius. Sm dwarfs and BCDs tend to have at least some blue and red radial color trend, respectively. Additionally, we determine stellar surface mass density (Σ) profiles and use them to show that the break in Σ generally remains in Type II dwarfs (unlike Type II spirals) but generally disappears in Type III dwarfs (unlike Type III spirals). Moreover, the break in Σ is strong, intermediate, and weak in faint dwarfs, bright dwarfs, and spirals, respectively, indicating that Σ may straighten with increasing galaxy mass. Finally, the average stellar surface mass density at the surface brightness break is roughly 1−2 M {sub ⊙} pc{sup −2} for Type II dwarfs but higher at 5.9 M {sub ⊙} pc{sup −2} or 27 M {sub ⊙} pc{sup −2} for
Profile structures of TJ-II stellarator plasmas

NARCIS (Netherlands)

Herranz, J.; Pastor, I.; Castejon, F.; de la Luna, E.; Garcia-Cortes, I.; Barth, C. J.; Ascasibar, E.; Sanchez, J.; Tribaldos, V.

2000-01-01

Fine structures are found in the TJ-II stellarator electron temperature and density profiles, when they are measured using a high spatial resolution Thomson scattering system. These structures consist of peaks and valleys superimposed to a smooth average. Some irregularities remain in an ensemble
THE PHOTOMETRIC AND KINEMATIC STRUCTURE OF FACE-ON DISK GALAXIES. II. INTEGRATED LINE PROFILE CHARACTERIZATION AND THE ORIGIN OF LINE PROFILE ASYMMETRY

International Nuclear Information System (INIS)

Andersen, David R.; Bershady, Matthew A.

2009-01-01

We perform a moments analysis of H I and H II global line profiles for 33 nearly face-on disk galaxies for the threefold purpose of rationalizing and interpreting line profile indices in the literature, presenting robust moment definitions with analytic error functions calibrated against Monte Carlo simulation, and probing the physical origin of line profile asymmetries. The first four profile moments serve as viable surrogates for the recession velocity, line width, asymmetry, and profile shape, respectively. The first three moments are superior, by a factor of ∼2 in precision, to related quantities defined in the literature. First and third profile moments are related; skew can be used to calculate more accurate recession velocities from global profiles. Second and fourth profile moments are linked, corresponding to the known trend that narrow (but well resolved) line widths tend to be more Gaussian. Hα kurtosis also appears correlated with the spatially resolved line width of the ionized gas. No systematics appear between various measures of line width and true rotation speed other than the wide range of normalizations, which we calibrate. This conclusion and calibration, however, is highly sample dependent. The ratio of H II to H I widths is consistent with unity, even at low projected line width. There may be a trend toward a decrease in the ratio of H II to H I widths consistent with previous studies, but we also observe greater scatter. While there is good agreement between H I and H II first, second, and fourth moments, we find no positive correlation between skew measured from H I and H II profiles. Detailed analysis of the spatially resolved Hα distribution demonstrates that H II global profile asymmetries are dominated by differences in the gas distribution, not kinematic asymmetries.
[Construction of porous hydroxyapatite (HA) block loaded with cultured chondrocytes].

Science.gov (United States)

Yan, M; Dang, G

1999-07-01

To construct a kind of bone healing enhancing implant with cultured chondrocytes bound to hydroxyapatite (HA). Chondrocytes were obtained from the costicartilage of rat and were cultured on the porous HA blocks, 3 mm x 3 mm x 4 mm size, for three and seven days. Scanning electron micrograph was taken to show whether the cells grew outside and inside the pore of HA block. The cells cultured on tiny glass sheet for 2 days were used to prove where the cells come from by in situ hybridization technique with alpha1 (II) cDNA probe. Scanning electron micrographs showed that the pores of the HA surface and inside of the blocks are filled with cultured cells, especially the longer cultured block. The cells were chondrocytes confirmed by in situ hybridization. The porous HA can be used as cell cultured substrate and chondrocyte can adhere and proliferate inside the porous HA block.
Sugar-binding sites of the HA1 subcomponent of Clostridium botulinum type C progenitor toxin.

Science.gov (United States)

Nakamura, Toshio; Tonozuka, Takashi; Ide, Azusa; Yuzawa, Takayuki; Oguma, Keiji; Nishikawa, Atsushi

2008-02-22

Clostridium botulinum type C 16S progenitor toxin contains a hemagglutinin (HA) subcomponent, designated HA1, which appears to play an important role in the effective internalization of the toxin in gastrointestinal epithelial cells and in creating a broad specificity for the oligosaccharide structure that corresponds to various targets. In this study, using the recombinant protein fused to glutathione S-transferase, we investigated the binding specificity of the HA1 subcomponent to sugars and estimated the binding sites of HA1 based on X-ray crystallography and soaking experiments using various sugars. N-Acetylneuraminic acid, N-acetylgalactosamine, and galactose effectively inhibited the binding that occurs between glutathione S-transferase-HA1 and mucins, whereas N-acetylglucosamine and glucose did not inhibit it. The crystal structures of HA1 complex with N-acetylneuraminic acid, N-acetylgalactosamine, and galactose were also determined. There are two sugar-binding sites, sites I and II. Site I corresponds to the electron densities noted for all sugars and is located at the C-terminal beta-trefoil domain, while site II corresponds to the electron densities noted only for galactose. An aromatic amino acid residue, Trp176, at site I has a stacking interaction with the hexose ring of the sugars. On the other hand, there is no aromatic residue at site II; thus, the interaction with galactose seems to be poor. The double mutant W176A at site I and D271F at site II has no avidity for N-acetylneuraminic acid but has avidity for galactose. In this report, the binding specificity of botulinum C16S toxin HA1 to various sugars is demonstrated based on its structural features.
The Influence of Class II Division 2 Malocclusions on the Harmony of the Human Face Profile.

Science.gov (United States)

Perović, Tatjana

2017-11-24

BACKGROUND Persons with class II division 2 malocclusion are characterized by a very specific dento-skeletal and soft-tissue profile (a profile in which a protruding nose and chin, retruding lips, concave and shortened lower third of the face, and gummy smile are dominant), which is the opposite of the currently modern profiles (convex profile of protruding lips and small chin). The aim of this research was to determine the differences in parameters of harmonies of facial profiles between persons with class II division 2 malocclusions and class I, and to establish the significance of those differences. MATERIAL AND METHODS For this study, 50 patients with class II division 2 malocclusions and 50 patients with class I were selected; profile photos were recorded and a photometric analysis was done: a type of profile according to Schwarz, the shape of a nose, the prominence of chin, biometrical field, the position of lips in relation to the tangent Sn-Pg, S-line (Steiner), E-line (Riketts) and a facial angle according to Arnett. RESULTS The significant differences in profiles of persons with class II division 2 compared to class I were: position and prominence of the chin, the position of the lower and upper lip in relation to the S-line, and smaller value of a facial angle in relation to persons with class I. CONCLUSIONS The differences seen in skeletal profiles were not associated with significant differences in the profiled facial contours of the examined groups. The compensatory role of the fullness of soft tissues of the lips is probably the reason why there were not significant deviations in all the examined parameters.
Line profile studies of hydrodynamical models of cometary compact H II regions

International Nuclear Information System (INIS)

Zhu, Feng-Yao; Zhu, Qing-Feng

2015-01-01

We simulate the evolution of cometary H II regions based on several champagne flow models and bow shock models, and calculate the profiles of the [Ne II] fine-structure line at 12.81 μm, the H30α recombination line and the [Ne III] fine-structure line at 15.55 μm for these models at different inclinations of 0°, 30° and 60°. We find that the profiles in the bow shock models are generally different from those in the champagne flow models, but the profiles in the bow shock models with lower stellar velocity (≤ 5 km s −1 ) are similar to those in the champagne flow models. In champagne flow models, both the velocity of peak flux and the flux weighted central velocities of all three lines point outward from molecular clouds. In bow shock models, the directions of these velocities depend on the speed of stars. The central velocities of these lines are consistent with the stellar motion in the high stellar speed cases, but they are opposite directions from the stellar motion in the low speed cases. We notice that the line profiles from the slit along the symmetrical axis of the projected 2D image of these models are useful for distinguishing bow shock models from champagne flow models. It is also confirmed by the calculation that the flux weighted central velocity and the line luminosity of the [Ne III] line can be estimated from the [Ne II] line and the H30α line. (paper)
New fuzzy EWMA control charts for monitoring phase II fuzzy profiles

Directory of Open Access Journals (Sweden)

Ghazale Moghadam

2016-01-01

Full Text Available In many quality control applications, the quality of a process or product is explained by the relationship between response variable and one or more explanatory variables, called a profile. In this paper, a new fuzzy EWMA control chart for phase II fuzzy profile monitoring is proposed. To this end, we extend EWMA control charts to its equivalent Fuzzy type and then implement fuzzy ranking methods to determine whether the process fuzzy profile is under or out of control. The proposed method is capable of identifying small changes in process under condition of process profile explaining parameters vagueness, roughness and uncertainty. Determining the source of changes, this method provides us with the possibility of recognizing the causes of process transition from stable mode, removing these causes and restoring the process stable mode.
Calculated Resonance Line Profiles of [Mg II], [C II], and [Si IV] in the Solar Atmosphere

Science.gov (United States)

Avrett, E.; Landi, E.; McKillop, S.

2013-12-01

NASA's Interface Region Imaging Spectrograph space mission, launched 2013 June 27, is intended to study the structure of the solar chromosphere and the transition region between the chromosphere and corona. The spectral lines to be observed include the Mg II k line at 2796.5 Å, the C II 1334.5 Å line, and the Si IV line at 1393.8 Å, which are formed in the middle chromosphere, the upper chromosphere, and the lower transition region, respectively. Here we calculate the profiles of these lines from four models of the solar atmosphere, intended to represent the faint and mean internetwork, a network lane, and bright network. We show how the profiles change from the center of the solar disk toward the limb of the Sun and in response to outflows and inflows. These results are intended to cover the range of expected quiet-Sun observations and assist in their interpretation. We expect that the observations will lead to improvements in the models, which can then be used to estimate the required non-radiative heating in the different regions.
Calculated resonance line profiles of [Mg II], [C II], and [Si IV] in the solar atmosphere

International Nuclear Information System (INIS)

Avrett, E.; McKillop, S.; Landi, E.

2013-01-01

NASA's Interface Region Imaging Spectrograph space mission, launched 2013 June 27, is intended to study the structure of the solar chromosphere and the transition region between the chromosphere and corona. The spectral lines to be observed include the Mg II k line at 2796.5 Å, the C II 1334.5 Å line, and the Si IV line at 1393.8 Å, which are formed in the middle chromosphere, the upper chromosphere, and the lower transition region, respectively. Here we calculate the profiles of these lines from four models of the solar atmosphere, intended to represent the faint and mean internetwork, a network lane, and bright network. We show how the profiles change from the center of the solar disk toward the limb of the Sun and in response to outflows and inflows. These results are intended to cover the range of expected quiet-Sun observations and assist in their interpretation. We expect that the observations will lead to improvements in the models, which can then be used to estimate the required non-radiative heating in the different regions.
Modification of NUR II neutron beam profile of MINT TRIGA MARK II research reactor for digital neutron radiography

International Nuclear Information System (INIS)

Muhammad Rawi Mohamed Zin; Azali Muhammad; Abdul Aziz Mohamed; Rafhayudi Jamro; Syed Nasaruddin Syed Idris; Ng Aik Hao; Rosly Jaafar

2006-01-01

A cone neutron beam collimated by a 5.4 cm aperture produced in the Neutron Radiography II (NUR II) via a step divergence collimator had to be modified to fulfill 5 cm x 6 cm dimension of the scintillation screen placed in the charge couple device (ccd) camera. The required convergence neutron beam was obtained by a simple collimator-beam plug plugged in front of the NUR II beam port. The calculations involved in designing the collimator-beam plug had to take into account not only the neutron beam profiling but also the neutron and gamma shielding and are discussed in this article. (Author)
Predictors of favorable soft tissue profile outcomes following Class II Twin-block treatment.

Science.gov (United States)

Kim, Ji-Eun; Mah, Su-Jung; Kim, Tae-Woo; Kim, Su-Jung; Park, Ki-Ho; Kang, Yoon-Goo

2018-01-01

The aim of this study was to determine cephalometric factors that help predict favorable soft-tissue profile outcomes following treatment with the Class II Twin-block appliance. Pre- and post-treatment lateral cephalograms of 45 patients treated with the Class II Twin-block appliance were retrospectively analyzed. Profile silhouettes were drawn from the cephalograms and evaluated by three orthodontists in order to determine the extent of improvement. Samples were divided into a favorable group (upper 30% of visual analogue scale [VAS] scores, n = 14) and an unfavorable group (lower 30% of VAS scores, n = 14). Skeletal and soft-tissue measurements were performed on the cephalograms and an intergroup comparison was conducted. An independent t -test revealed that the following pre-treatment values were lower in the favorable group compared to the unfavorable group: lower incisor to mandibular plane angle, lower incisor to pogonion distance, point A-nasion-point B angle, sella-nasion line (SN) to maxillary plane angle, SN to mandibular plane angle, gonial angle, and symphysis inclination. The favorable group had a larger incisor inclination to occlusal plane. Moreover, the favorable group showed larger post-treatment changes in gonial angle, B point projection, and pogonion projection than did the unfavorable group. Class II malocclusion patients with a low divergent skeletal pattern and reduced lower incisor protrusions are likely to show more improvement in soft-tissue profile outcomes following Class II Twin-block treatment.
Atmospheric structure deduced from disturbed line profiles application to Ca II lines

International Nuclear Information System (INIS)

Mein, N.; Mein, P.; Malherbe, J.-M.; Dame, L.; Dumont, S.; CNRS, Laboratoire de Physique Stellaire et Planetaire, Verrieres-le-Buisson, France; College de France, Paris)

1985-01-01

A new method is described in order to derive physical quantities (temperature, pressure, radial velocities) from the observation of disturbed line profiles. A method of Fourier analysis is suggested with double profiles and a nonlinear expansion of the coefficient of the Fourier terms. An application to a sequence of H-Ca II lines is attempted. The method is a powerful tool allowing for the simultaneous determination of at least four physical quantities. 9 references
Development and characterization of poli composites (ether ether ketone)(PEEK)(Hydroxyapatite(HA); Desenvolvimento e caracterizacao de compositos poli (eter-eter-cetona)(PEEK)/Hidroxiapatita(HA)

Energy Technology Data Exchange (ETDEWEB)

Ferreira, V.P.; Santos, F.S.F.; Sa, M.D. de; Fook, M.V.L., E-mail: valeriap.ferreira@hotmail.com [Universidade Federal de Campina Grande (UFCG), PB (Brazil). Centro de Ciencias e Tecnologia. Programa de Pos-Graduacao em Ciencia e Engenharia de Materiais

2016-07-01

The objective of this work was to develop PEEK / HA composites, combining the biological activity of the ceramic phase with the properties of the polymer phase, the materials used in this research were Poly (ether-ether-ketone) (PEEK) and Hydroxyapatite (HA) (50, 60, 70 and 80% m / v HA), this material was subjected to a load of two tons followed by a thermal treatment at 390 ° for a period of 30 minutes. Then they were characterized by FTIR, DRX and MO. In the physical-chemical characterization of FTIR and XRD, it was not possible to identify significant alterations. In the FTIR spectra of the composites, there is no formation of new identifiable chemical bonds. In the composites XRD diffractograms a profile similar to the ceramic phase was observed, with peaks increasing in intensity and narrowing proportional to the increase of the hydroxyapatite concentration in the composites. In optical microscopy it is possible to observe surfaces with heterogeneous morphology, with signs of roughness and in the cross section we observe a heterogeneous aspect, rich in regions with large agglomerates and lighter particles. Considering the processing aspects, the technique proved to be effective for the development of PEEK /HA composites. (author)

Probable maximum flood on the Ha Ha River

International Nuclear Information System (INIS)

Damov, D.; Masse, B.

1997-01-01

Results of a probable maximum flood (PMF) study conducted for various locations along the Ha Ha river, a tributary of the Saguenay River, were discussed. The study was undertaken for use in the design and construction of new hydraulic structures for water supply for a pulp and paper facility, following the Saguenay Flood in July 1996. Many different flood scenarios were considered, including combinations of snow-melt with rainfall. Using computer simulations, it was shown that the largest flood flows were generated by summer-fall PMF. 5 refs., 12 figs
The Study on Biocompatibility of Porous nHA/PLGA Composite Scaffolds for Tissue Engineering with Rabbit Chondrocytes In Vitro

Directory of Open Access Journals (Sweden)

Lei Chen

2013-01-01

Full Text Available Objective. To examine the biocompatibility of a novel nanohydroxyapatite/poly[lactic-co-glycolic acid] (nHA/PLGA composite and evaluate its feasibility as a scaffold for cartilage tissue engineering. Methods. Chondrocytes of fetal rabbit were cultured with nHA/PLGA scaffold in vitro and the cell viability was assessed by MTT assay first. Cells adhering to nHA/PLGA scaffold were then observed by inverted microscope and scanning electron microscope (SEM. The cell cycle profile was analyzed by flow cytometry. Results. The viability of the chondrocytes on the scaffold was not affected by nHA/PLGA comparing with the control group as it was shown by MTT assay. Cells on the surface and in the pores of the scaffold increased in a time-dependent manner. Results obtained from flow cytometry showed that there was no significant difference in cell cycle profiles between the coculture group and control (P>0.05. Conclusion. The porous nHA/PLGA composite scaffold is a biocompatible and good kind of scaffold for cartilage tissue engineering.
Iterative noise removal from temperature and density profiles in the TJ-II Thomson scattering

International Nuclear Information System (INIS)

Farias, G.; Dormido-Canto, S.; Vega, J.; Santos, M.; Pastor, I.; Fingerhuth, S.; Ascencio, J.

2014-01-01

TJ-II Thomson Scattering diagnostic provides temperature and density profiles of plasma. The CCD camera acquires images that are corrupted with some kind of noise called stray-light. This noise degrades both image contrast and measurement accuracy, which could produce unreliable profiles of the diagnostic. So far, several approaches have been applied in order to decrease the noise in the TJ-II Thomson scattering images. Since the presence of the noise is not global but located in some particular regions of the image, advanced processing techniques are needed. However such methods require of manual fine-tuning of parameters to reach a good performance. In this contribution, an iterative image processing approach is applied in order to reduce the stray light effects in the images of the TJ-II Thomson scattering diagnostic. The proposed solution describes how the noise can be iteratively reduced in the images when a key parameter is automatically adjusted during the iterative process
Iterative noise removal from temperature and density profiles in the TJ-II Thomson scattering

Energy Technology Data Exchange (ETDEWEB)

Farias, G., E-mail: gonzalo.farias@ucv.cl [Pontificia Universidad Católica de Valparaíso, Av. Brasil 2147, Valparaíso (Chile); Dormido-Canto, S., E-mail: sebas@dia.uned.es [Departamento de Informática y Automática, UNED, 28040 Madrid (Spain); Vega, J., E-mail: jesus.vega@ciemat.es [Asociación EURATOM/CIEMAT para Fusión, Avd. Complutense 22, 28040 Madrid (Spain); Santos, M., E-mail: msantos@ucm.es [Departamento de Arquitectura de Computadores y Automática, Universidad Complutense de Madrid, 28040 Madrid (Spain); Pastor, I., E-mail: ignacio.pastor@ciemat.es [Asociación EURATOM/CIEMAT para Fusión, Avd. Complutense 22, 28040 Madrid (Spain); Fingerhuth, S., E-mail: sebastian.fingerhuth@ucv.cl [Pontificia Universidad Católica de Valparaíso, Av. Brasil 2147, Valparaíso (Chile); Ascencio, J., E-mail: j_ascencio21@hotmail.com [Pontificia Universidad Católica de Valparaíso, Av. Brasil 2147, Valparaíso (Chile)

2014-05-15

TJ-II Thomson Scattering diagnostic provides temperature and density profiles of plasma. The CCD camera acquires images that are corrupted with some kind of noise called stray-light. This noise degrades both image contrast and measurement accuracy, which could produce unreliable profiles of the diagnostic. So far, several approaches have been applied in order to decrease the noise in the TJ-II Thomson scattering images. Since the presence of the noise is not global but located in some particular regions of the image, advanced processing techniques are needed. However such methods require of manual fine-tuning of parameters to reach a good performance. In this contribution, an iterative image processing approach is applied in order to reduce the stray light effects in the images of the TJ-II Thomson scattering diagnostic. The proposed solution describes how the noise can be iteratively reduced in the images when a key parameter is automatically adjusted during the iterative process.
Crystal structure of the HA3 subcomponent of Clostridium botulinum type C progenitor toxin.

Science.gov (United States)

Nakamura, Toshio; Kotani, Mao; Tonozuka, Takashi; Ide, Azusa; Oguma, Keiji; Nishikawa, Atsushi

2009-01-30

The Clostridium botulinum type C 16S progenitor toxin contains a neurotoxin and several nontoxic components, designated nontoxic nonhemagglutinin (HA), HA1 (HA-33), HA2 (HA-17), HA3a (HA-22-23), and HA3b (HA-53). The HA3b subcomponent seems to play an important role cooperatively with HA1 in the internalization of the toxin by gastrointestinal epithelial cells via binding of these subcomponents to specific oligosaccharides. In this study, we investigated the sugar-binding specificity of the HA3b subcomponent using recombinant protein fused to glutathione S-transferase and determined the three-dimensional structure of the HA3a-HA3b complex based on X-ray crystallography. The crystal structure was determined at a resolution of 2.6 A. HA3b contains three domains, domains I to III, and the structure of domain I resembles HA3a. In crystal packing, three HA3a-HA3b molecules are assembled to form a three-leaved propeller-like structure. The three HA3b domain I and three HA3a alternate, forming a trimer of dimers. In a database search, no proteins with high structural homology to any of the domains (Z score >10) were found. Especially, HA3a and HA3b domain I, mainly composed of beta-sheets, reveal a unique fold. In binding assays, HA3b bound sialic acid with high affinity, but did not bind galactose, N-acetylgalactosamine, or N-acetylglucosamine. The electron density of liganded N-acetylneuraminic acid was determined by crystal soaking. In the sugar-complex structure, the N-acetylneuraminic acid-binding site was located in the cleft formed between domains II and III of HA3b. This report provides the first determination of the three-dimensional structure of the HA3a-HA3b complex and its sialic acid binding site. Our results will provide useful information for elucidating the mechanism of assembly of the C16S toxin and for understanding the interactions with oligosaccharides on epithelial cells and internalization of the botulinum toxin complex.
HA-Coated Implant

DEFF Research Database (Denmark)

Daugaard, Henrik; Søballe, Kjeld; Bechtold, Joan E

2014-01-01

of improving the fixation of implants. Of these, hydroxyapatite (HA) is the most widely used and most extensively investigated. HA is highly osseoconductive, and the positive effect is well documented in both basic and long-term clinical research [1–6]. This chapter describes experimental and clinical studies...
Morphological Effects of HA on the Cell Compatibility of Electrospun HA/PLGA Composite Nanofiber Scaffolds

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Adnan Haider

2014-01-01

Full Text Available Tissue engineering is faced with an uphill challenge to design a platform with appropriate topography and suitable surface chemistry, which could encourage desired cellular activities and guide bone tissue regeneration. To develop such scaffolds, composite nanofiber scaffolds of nHA and sHA with PLGA were fabricated using electrospinning technique. nHA was synthesized using precipitation method, whereas sHA was purchased. The nHA and sHA were suspended in PLGA solution separately and electrospun at optimized electrospinning parameters. The composite nanofiber scaffolds were characterized by FE-SEM, EDX analysis, TEM, XRD analysis, FTIR, and X-ray photoelectron. The potential of the HA/PLGA composite nanofiber as bone scaffolds in terms of their bioactivity and biocompatibility was assessed by culturing the osteoblastic cells onto the composite nanofiber scaffolds. The results from in vitro studies revealed that the nHA/PLGA composite nanofiber scaffolds showed higher cellular adhesion, proliferation, and enhanced osteogenesis performance, along with increased Ca+2 ions release compared to the sHA/PLGA composite nanofiber scaffolds and pristine PLGA nanofiber scaffold. The results show that the structural dependent property of HA might affect its potential as bone scaffold and implantable materials in regenerative medicine and clinical tissue engineering.
Preparation, characterization of electrospun meso-hydroxylapatite nanofibers and their sorptions on Co(II)

Energy Technology Data Exchange (ETDEWEB)

Wang, Hualin, E-mail: hlwang@hfut.edu.cn [School of Chemical Technology, Hefei University of Technology, Hefei, Anhui 230009 (China); Zhang, Peng; Ma, Xingkong; Jiang, Suwei; Huang, Yan; Zhai, Linfeng [School of Chemical Technology, Hefei University of Technology, Hefei, Anhui 230009 (China); Jiang, Shaotong [School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui 230009 (China)

2014-01-30

Highlights: • PVA/HA nanofibers could change into meso-HA nanofibers by calcination process. • Sorption of Co(II) on meso-HA was strongly dependent on pH and ionic strength. • Sorption kinetic data were well fitted by the pseudo-second-order rate equation. • Sorption isotherms could be well described by the Langmuir model. • Sorption process of Co(II) on meso-HA nanofibers was spontaneous and endothermic. -- Abstract: In this work, mesoporous hydroxylapatite (meso-HA) nanofibers were prepared via calcination process with polyvinyl alcohol/HA (PVA/HA) hybrid nanofibers fabricated by electrospinning technique as precursors, and the removal efficiency of meso-HA nanofibers toward Co(II) was evaluated via sorption kinetics and sorption isotherms. Furthermore, the sorption behaviors of Co(II) on meso-HA nanofibers were explored as a function of pH, ionic strength, and thermodynamic parameters. There existed hydrogen bonds between HA and PVA matrix in precursor nanofibers which could change into meso-HA nanofibers with main pore diameter at 27 nm and specific surface area at 114.26 m{sup 2}/g by calcination process. The sorption of Co(II) on meso-HA was strongly dependent on pH and ionic strength. Outer-sphere surface complexation or ion exchange was the main mechanisms of Co(II) adsorption on meso-HA at low pH, whereas inner-sphere surface complexation was the main adsorption mechanism at high pH. The sorption kinetic data were well fitted by the pseudo-second-order rate equation. The sorption isotherms could be well described by the Langmuir model. The thermodynamic parameters (ΔH°, ΔS° and ΔG°) calculated from the temperature-dependent sorption isotherms suggested that the sorption process of Co(II) on meso-HA nanofibers was spontaneous and endothermic.
Design and fabrication of carbon fibers with needle-like nano-HA coating to reinforce granular nano-HA composites.

Science.gov (United States)

Wang, Xudong; Zhao, Xueni; Zhang, Li; Wang, Wanying; Zhang, Jing; He, Fuzhen; Yang, Jianjun

2017-08-01

Carbon fibers (CFs) with needle-like nano-hydroxyapatite (nHA) coating were first used as reinforcing materials named nHA-CFs to improve the mechanical properties of pure HA. A powder mixture containing nHA-CFs and granular nano-HA (gHA) was directly sintered by hot pressing at appropriate sintering pressure and temperature. A three-phase nHA-CFs/gHA composite was designed, fabricated, and used as an artificial bone. Results show that the bending strengths of the nHA-CFs/gHA composite are approximately 41.1% and 59.2% higher than those of CFs/gHA composite and pure HA, respectively. The possible reinforcing mechanism of nHA-CFs in the composite is also proposed at the end. When nHA-CFs are applied for preparation of nHA-CFs/gHA composites, the internal stress on its phase boundary with gHA matrix generated during cooling of sintered is significantly reduced due to the presence of the nHA coatings. It infers that nHA coatings on CFs might act as a bridge to control the forming of interfacial gaps between the gHA matrix and the CFs effectively. Our work provides additional insights into the feasibility of nHA-CFs/gHA composites as load-bearing implant materials in clinical applications. Copyright © 2017 Elsevier B.V. All rights reserved.
Lipid profile abnormalities in Type II diabetics with and without microalbuminura

International Nuclear Information System (INIS)

Qadoos, A.; Javaid, Q.U.A.; Shahzad, A.; Toor, I.

2017-01-01

To find the frequency of microalbuminemia in type II diabetic patients and to determine the frequency of different abnormalities of lipid profile in Type II diabetic patients with and without microalbuminuria. Methodology: Total 344 patients of type-II diabetes mellitus of both genders between the age of 35-70 years were enrolled in the study from Lahore General Hospital, Lahore, Pakistan. Blood sample for lipid profile was collected after 12 hours of fasting and spot mid stream early morning urine sample was collected in laboratory for microalbuminuria detection. Results: Out of 344 cases, 151 (43.90%) were male and 193 (56.10%) were females with male to female ratio of 2:1. 132 (38.37%) cases were between 35-50 years of age while 212 (61.63%) between 51-70 years of age (mean 53.18+9.32). Frequency of microalbuminuria was recorded in 32.56% (n=112) cases. Out of 112 cases of microalbuminuria, Hypertriglyceridemia was seen in 98 (87.5%) cases, increased LDL-C in 102 (91%) while decreased HDL-C was found in 95 (84.8%) cases (p=0.000). Out of 232 cases of without microalbuminuria, hypertriglyceridemia was found in 143 (61%) cases, increased LDL-C in 151 (65%) and decreased HDL-C was seen in 169 (72.8%) cases. Frequency of microalbuminuria was seen more in age group of 51-75 years (n=65) with disease duration of more than 3 years (n=71), HbA1c 6 (n=69) and BMI more than 30. Conclusion: The frequency of hypertriglyceridemia, increased LDL-C and decreased HDL-C is higher in type II diabetic patients with microalbuminuria as compare to patients without microalbuminuria and is dependent on advanced age, duration of disease and BMI. (author)
The Influence of Cultivation System on Distribution Profile Of 137cs and Erosion / Deposition Rate

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Nita Suhartini

2016-05-01

Full Text Available 137Cs radiogenic content in the soil can be used to estimate the rate of erosion and deposition in an area occurring since 1950’s, by comparing the content of the 137Cs in observed site with those in a stable reference site. This experiment aimed to investigate the influence of cultivation type on distribution profile of 137Cs and distribution of erosion and deposition rate in cultivated area. A study site was small cultivated area with slope steepness <10o and length 2 km located in Bojong – Ciawi. For this purpose, the top of a slope was chosen for reference site and three plot sites were selected namely Land Use I that using simple cultivation, Land Use II that using simple cultivation with ridge and furrow, and Land Use III using machine cultivation. The results showed that cultivation could make a movement of 137Cs to the deeper layer and ridges and furrows cultivation system could minimized an erosion process. The net erosion and deposition for land Use I, II and III were -25 t/ha/yr , 24 t/ha/yr and -58 t/ha/yr, respectively.
Effect of n-HA with different surface-modified on the properties of n-HA/PLGA composite

Energy Technology Data Exchange (ETDEWEB)

Jiang Liuyun, E-mail: jlytxg@163.com [Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041 (China); Xiong Chengdong; Chen Dongliang [Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041 (China); Jiang Lixin [Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041 (China); Graduated School of Chinese Academy of Sciences, Beijing 100039 (China); Pang Xiubing [Zhejiang Apeloa Medical Technology Co. Ltd, Jinhua 322118 (China)

2012-10-15

Graphical abstract: The bend strength of n-HA/PLGA composite with the unmodified n-HA becomes lower than that of PLGA. However, when n-HA was modified by different methods, the bend strength of g-n-HA/PLGA composites gets a little increase than PLGA, and the g3-n-HA/PLGA shows the highest bend strength at 3% g3-n-HA loading amount in weight, reached 162 MPa, which was 24.4% higher than that of pure PLGA. Highlights: Black-Right-Pointing-Pointer A new surface modification method for n-HA of combining stearic acid with surface-grafting L-lactic was adopted. Black-Right-Pointing-Pointer Three different surface modification methods for n-HA were compared in detail. Black-Right-Pointing-Pointer The new surface modification method was the most ideal method in this study. Black-Right-Pointing-Pointer The g3-n-HA/PLGA composite had the highest bending strength, which would be potential to be used as bone fracture internal fixation materials. - Abstract: Three different surface modification methods for nano-hydroxyapatite (n-HA) of stearic acid, grafted with L-lactide, combining stearic acid and surface-grafting L-lactic were adopted, respectively. The surface modification reaction and the effect of different methods were evaluated by Fourier transformation infrared (FTIR), X-ray photoelectron spectra (XPS), thermal gravimetric analysis (TGA), transmission electron microscopy (TEM). The results showed that n-HA surfaces were all successful modified, and the modification method of combining stearic acid and surface-grafting L-lactic had the greatest grafting amount and the best dispersion among the three modification methods. Then, the n-HA with three different surface modification and unmodified n-HA were introduced into PLGA, respectively, and a serials of n-HA/PLGA composites with 3% n-HA amount in weight were prepared by solution mixing, and the properties of n-HA/PLGA composites were also investigated by electromechanical universal tester and scanning electron
Characterization of Three Novel Fatty Acid- and Retinoid-Binding Protein Genes (Ha-far-1, Ha-far-2 and Hf-far-1 from the Cereal Cyst Nematodes Heterodera avenae and H. filipjevi.

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Fen Qiao

Full Text Available Heterodera avenae and H. filipjevi are major parasites of wheat, reducing production worldwide. Both are sedentary endoparasitic nematodes, and their development and parasitism depend strongly on nutrients obtained from hosts. Secreted fatty acid- and retinol-binding (FAR proteins are nematode-specific lipid carrier proteins used for nutrient acquisition as well as suppression of plant defenses. In this study, we obtained three novel FAR genes Ha-far-1 (KU877266, Ha-far-2 (KU877267, Hf-far-1 (KU877268. Ha-far-1 and Ha-far-2 were cloned from H. avenae, encoding proteins of 191 and 280 amino acids with molecular masses about 17 and 30 kDa, respectively and sequence identity of 28%. Protein Blast in NCBI revealed that Ha-FAR-1 sequence is 78% similar to the Gp-FAR-1 protein from Globodera pallida, while Ha-FAR-2 is 30% similar to Rs-FAR-1 from Radopholus similis. Only one FAR protein Hf-FAR-1was identified in H. filipjevi; it had 96% sequence identity to Ha-FAR-1. The three proteins are alpha-helix-rich and contain the conserved domain of Gp-FAR-1, but Ha-FAR-2 had a remarkable peptide at the C-terminus which was random-coil-rich. Both Ha-FAR-1 and Hf-FAR-1 had casein kinase II phosphorylation sites, while Ha-FAR-2 had predicted N-glycosylation sites. Phylogenetic analysis showed that the three proteins clustered together, though Ha-FAR-1 and Hf-FAR-1 adjoined each other in a plant-parasitic nematode branch, but Ha-FAR-2 was distinct from the other proteins in the group. Fluorescence-based ligand binding analysis showed the three FAR proteins bound to a fluorescent fatty acid derivative and retinol and with dissociation constants similar to FARs from other species, though Ha-FAR-2 binding ability was weaker than that of the two others. In situ hybridization detected mRNAs of Ha-far-1 and Ha-far-2 in the hypodermis. The qRT-PCR results showed that the Ha-far-1and Ha-far-2 were expressed in all developmental stages; Ha-far-1 expressed 70 times more
Characterization of Three Novel Fatty Acid- and Retinoid-Binding Protein Genes (Ha-far-1, Ha-far-2 and Hf-far-1) from the Cereal Cyst Nematodes Heterodera avenae and H. filipjevi.

Science.gov (United States)

Qiao, Fen; Luo, Lilian; Peng, Huan; Luo, Shujie; Huang, Wenkun; Cui, Jiangkuan; Li, Xin; Kong, Lingan; Jiang, Daohong; Chitwood, David J; Peng, Deliang

2016-01-01

Heterodera avenae and H. filipjevi are major parasites of wheat, reducing production worldwide. Both are sedentary endoparasitic nematodes, and their development and parasitism depend strongly on nutrients obtained from hosts. Secreted fatty acid- and retinol-binding (FAR) proteins are nematode-specific lipid carrier proteins used for nutrient acquisition as well as suppression of plant defenses. In this study, we obtained three novel FAR genes Ha-far-1 (KU877266), Ha-far-2 (KU877267), Hf-far-1 (KU877268). Ha-far-1 and Ha-far-2 were cloned from H. avenae, encoding proteins of 191 and 280 amino acids with molecular masses about 17 and 30 kDa, respectively and sequence identity of 28%. Protein Blast in NCBI revealed that Ha-FAR-1 sequence is 78% similar to the Gp-FAR-1 protein from Globodera pallida, while Ha-FAR-2 is 30% similar to Rs-FAR-1 from Radopholus similis. Only one FAR protein Hf-FAR-1was identified in H. filipjevi; it had 96% sequence identity to Ha-FAR-1. The three proteins are alpha-helix-rich and contain the conserved domain of Gp-FAR-1, but Ha-FAR-2 had a remarkable peptide at the C-terminus which was random-coil-rich. Both Ha-FAR-1 and Hf-FAR-1 had casein kinase II phosphorylation sites, while Ha-FAR-2 had predicted N-glycosylation sites. Phylogenetic analysis showed that the three proteins clustered together, though Ha-FAR-1 and Hf-FAR-1 adjoined each other in a plant-parasitic nematode branch, but Ha-FAR-2 was distinct from the other proteins in the group. Fluorescence-based ligand binding analysis showed the three FAR proteins bound to a fluorescent fatty acid derivative and retinol and with dissociation constants similar to FARs from other species, though Ha-FAR-2 binding ability was weaker than that of the two others. In situ hybridization detected mRNAs of Ha-far-1 and Ha-far-2 in the hypodermis. The qRT-PCR results showed that the Ha-far-1and Ha-far-2 were expressed in all developmental stages; Ha-far-1 expressed 70 times more than Ha-far-2 in
SURFACE BRIGHTNESS PROFILES OF DWARF GALAXIES. I. PROFILES AND STATISTICS

International Nuclear Information System (INIS)

Herrmann, Kimberly A.; Hunter, Deidre A.; Elmegreen, Bruce G.

2013-01-01

Radial surface brightness profiles of spiral galaxies are classified into three types: (I) single exponential, or the light falls off with one exponential to a break before falling off (II) more steeply, or (III) less steeply. Profile breaks are also found in dwarf disks, but some dwarf Type IIs are flat or increasing out to a break before falling off. Here we re-examine the stellar disk profiles of 141 dwarfs: 96 dwarf irregulars (dIms), 26 Blue Compact Dwarfs (BCDs), and 19 Magellanic-type spirals (Sms). We fit single, double, or even triple exponential profiles in up to 11 passbands: GALEX FUV and NUV, ground-based UBVJHK and Hα, and Spitzer 3.6 and 4.5 μm. We find that more luminous galaxies have brighter centers, larger inner and outer scale lengths, and breaks at larger radii; dwarf trends with M B extend to spirals. However, the V-band break surface brightness is independent of break type, M B , and Hubble type. Dwarf Type II and III profiles fall off similarly beyond the breaks but have different interiors and IIs break ∼twice as far as IIIs. Outer Type II and III scale lengths may have weak trends with wavelength, but pure Type II inner scale lengths clearly decrease from the FUV to visible bands whereas Type III inner scale lengths increase with redder bands. This suggests the influence of different star formation histories on profile type, but nonetheless the break location is approximately the same in all passbands. Dwarfs continue trends between profile and Hubble types such that later-type galaxies have more Type II but fewer Type I and III profiles than early-type spirals. BCDs and Sms are over-represented as Types III and II, respectively, compared to dIms
Development and characterization of poli composites (ether ether ketone)(PEEK)(Hydroxyapatite(HA)

International Nuclear Information System (INIS)

Ferreira, V.P.; Santos, F.S.F.; Sa, M.D. de; Fook, M.V.L.

2016-01-01

The objective of this work was to develop PEEK / HA composites, combining the biological activity of the ceramic phase with the properties of the polymer phase, the materials used in this research were Poly (ether-ether-ketone) (PEEK) and Hydroxyapatite (HA) (50, 60, 70 and 80% m / v HA), this material was subjected to a load of two tons followed by a thermal treatment at 390 ° for a period of 30 minutes. Then they were characterized by FTIR, DRX and MO. In the physical-chemical characterization of FTIR and XRD, it was not possible to identify significant alterations. In the FTIR spectra of the composites, there is no formation of new identifiable chemical bonds. In the composites XRD diffractograms a profile similar to the ceramic phase was observed, with peaks increasing in intensity and narrowing proportional to the increase of the hydroxyapatite concentration in the composites. In optical microscopy it is possible to observe surfaces with heterogeneous morphology, with signs of roughness and in the cross section we observe a heterogeneous aspect, rich in regions with large agglomerates and lighter particles. Considering the processing aspects, the technique proved to be effective for the development of PEEK /HA composites. (author)
Adhesive strength of hydroxyl apatite(HA coating and biomechanics behavior of HA-coated prosthesis:an experimental study

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Tian-yang ZHANG

2011-05-01

Full Text Available Objective To explore the influence of adhesive strength of hydroxyapatite(HA coating on the post-implantation stability of HA-coated prosthesis.Methods The adhesive strength and biomechanics behavior of HA coating were studied by histopathological observation,material parameters and biomechanical testing,the titanium(Ti-coated prosthesis was employed as control.Results Scratch test showed that the adhesive strength of HA coating was significantly lower than that of Ti coating(P < 0.01.Histopathological examination and bone morphometry showed that,at the early stage of prosthesis implantation,the bony growth around HA-coated prosthesis was significantly higher than that around Ti-coated prosthesis(P < 0.01,but the ultimate shear strength of HA-coated prosthesis was much lower than that of Ti-coated prosthesis(P < 0.01.After the push-out test with prosthesis,histopathological observation showed that there were accumulations of clump-and strip-like granular residues on the surface of bones that newly grew around the HA-coated prosthesis,and surface energy-dispersive X-ray spectroscopy(EDX analysis also confirmed that the shear stress induced HA decohesion from the substrate of prosthesis.Conclusions Although HA coating showed a satisfactory effect on early bone formation and prosthetic stability,due to the deficiencies of adhesive strength,the early stability of prosthesis may be gradually destroyed by the shear loads of human body and coating degradation.
Gene Expression Profile in the Early Stage of Angiotensin II-induced Cardiac Remodeling: a Time Series Microarray Study in a Mouse Model

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Meng-Qiu Dang

2015-01-01

Full Text Available Background/Aims: Angiotensin II (Ang II plays a critical role in the cardiac remodeling contributing to heart failure. However, the gene expression profiles induced by Ang II in the early stage of cardiac remodeling remain unknown. Methods: Wild-type male mice (C57BL/6 background, 10-weeek-old were infused with Ang II (1500 ng/kg/min for 7 days. Blood pressure was measured. Cardiac function and remodeling were examined by echocardiography, H&E and Masson staining. The time series microarrays were then conducted to detected gene expression profiles. Results: Microarray results identified that 1,489 genes were differentially expressed in the hearts at day 1, 3 and 7 of Ang II injection. These genes were further classified into 26 profiles by hierarchical cluster analysis. Of them, 4 profiles were significant (No. 19, 8, 21 and 22 and contained 904 genes. Gene Ontology showed that these genes mainly participate in metabolic process, oxidation-reduction process, extracellular matrix organization, apoptotic process, immune response, and others. Significant pathways included focal adhesion, ECM-receptor interaction, cytokine-cytokine receptor interaction, MAPK and insulin signaling pathways, which were known to play important roles in Ang II-induced cardiac remodeling. Moreover, gene co-expression networks analysis suggested that serine/cysteine peptidase inhibitor, member 1 (Serpine1, also known as PAI-1 localized in the core of the network. Conclusions: Our results indicate that many genes are mainly involved in metabolism, inflammation, cardiac fibrosis and hypertrophy. Serpine1 may play a central role in the development of Ang II-induced cardiac remodeling at the early stage.
Adhesive strength of hydroxyl apatite(HA) coating and biomechanics behavior of HA-coated prosthesis:an experimental study

OpenAIRE

Tian-yang ZHANG; Yong-hong DUAN; Shu ZHU; Jin-yu ZHU; Qing-sheng ZHU

2011-01-01

Objective To explore the influence of adhesive strength of hydroxyapatite(HA) coating on the post-implantation stability of HA-coated prosthesis.Methods The adhesive strength and biomechanics behavior of HA coating were studied by histopathological observation,material parameters and biomechanical testing,the titanium(Ti)-coated prosthesis was employed as control.Results Scratch test showed that the adhesive strength of HA coating was significantly lower than that of Ti coating(P < 0.01).Hist...
The HaDREB2 transcription factor enhances basal thermotolerance and longevity of seeds through functional interaction with HaHSFA9

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Carranco Raúl

2009-06-01

Full Text Available Abstract Background Transcription factor HaDREB2 was identified in sunflower (Helianthus annuus L. as a drought-responsive element-binding factor 2 (DREB2 with unique properties. HaDREB2 and the sunflower Heat Shock Factor A9 (HaHSFA9 co-activated the Hahsp17.6G1 promoter in sunflower embryos. Both factors could be involved in transcriptional co-activation of additional small heat stress protein (sHSP promoters, and thus contribute to the HaHSFA9-mediated enhancement of longevity and basal thermotolerance of seeds. Results We found that overexpression of HaDREB2 in seeds did not enhance longevity. This was deduced from assays of basal thermotolerance and controlled seed-deterioration, which were performed with transgenic tobacco. Furthermore, the constitutive overexpression of HaDREB2 did not increase thermotolerance in seedlings or result in the accumulation of HSPs at normal growth temperatures. In contrast, when HaDREB2 and HaHSFA9 were conjointly overexpressed in seeds, we observed positive effects on seed longevity, beyond those observed with overexpression of HaHSFA9 alone. Such additional effects are accompanied by a subtle enhancement of the accumulation of subsets of sHSPs belonging to the CI and CII cytosolic classes. Conclusion Our results reveal the functional interdependency of HaDREB2 and HaHSFA9 in seeds. HaDREB2 differs from other previously characterized DREB2 factors in plants in terms of its unique functional interaction with the seed-specific HaHSFA9 factor. No functional interaction between HaDREB2 and HaHSFA9 was observed when both factors were conjointly overexpressed in vegetative tissues. We therefore suggest that additional, seed-specific factors, or protein modifications, could be required for the functional interaction between HaDREB2 and HaHSFA9.

Removal of Hg(II) from aqueous solution using sodium humate as heavy metal capturing agent.

Science.gov (United States)

Wang, Shixiang; Liu, Yong; Fan, Qin; Zhou, Anlan; Fan, Lu; Mu, Yulan

2016-12-01

An environmental friendly and economic natural biopolymer-sodium humate (HA-Na) was used to capture Hg(II) from aqueous solutions, and the trapped Hg(II) (HA-Na-Hg) was then removed by aluminium coagulation. The best Hg(II) capturing performance (90.60%) was observed under the following conditions: initial pH of 7.0, coagulation pH of 6.0, HA-Na dosage of 5.0 g L -1 , Al 2 (SO 4 ) 3 .18H 2 O dosage of 4.0 g L -1 , initial Hg(II) concentration of 50 mg L -1 and capturing time of 30 min. The HA-Na compositions with the molecular weight beyond 70 kDa showed the most intense affinity toward Hg(II). The results showed that the reaction equilibrium was achieved within 10 min (pH 7.0), and could be well fitted by the pseudo-second-order kinetics model. The capturing process could be well described by the Langmuir isotherm model and the maximum capturing capacity of Hg(II) was high up to 9.80 mg g -1 at 298 K (pH 7.0). The Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analysis showed that the redox reaction between Hg(II) and HA-Na and the coordination reaction of carboxyl and hydroxy groups of HA-Na with Hg(II) were responsible for Hg(II) removal. The successive regeneration experiment showed that the capturing efficiency of humates for Hg(II) was maintained at about 51% after five capture-regeneration recycles.
Perceived facial changes of Class II Division 1 patients with convex profiles after functional orthopedic treatment followed by fixed orthodontic appliances.

Science.gov (United States)

Tsiouli, Kleopatra; Topouzelis, Nikolaos; Papadopoulos, Moschos A; Gkantidis, Nikolaos

2017-07-01

The aim of this research was to investigate the perceived facial changes in Class II Division 1 patients with convex profiles after functional orthopedic treatment followed by fixed orthodontic appliances. Pretreatment and posttreatment profile photographs of 12 Class II Division 1 patients treated with activators, 12 Class II Division 1 patients treated with Twin-block appliances, and 12 controls with normal profiles treated without functional appliances were presented in pairs to 10 orthodontists, 10 patients, 10 parents, and 10 laypersons. The raters assessed changes in facial appearance on a visual analog scale. Two-way multivariate analysis of variance was used to evaluate differences among group ratings. Intrarater reliability was strong in most cases (intraclass correlation coefficients, >0.7). The internal consistency of the assessments was high (alpha, >0.87), both within and between groups. The raters consistently perceived more positive changes in the Class II Division 1 groups compared with the control group. However, this difference hardly exceeded 1/10th of the total visual analog scale length in its highest value and was mostly evident in the lower face and chin. No significant differences were found between the activator and the Twin-block groups. Although the raters perceived improvements of the facial profiles after functional orthopedic treatment followed by fixed orthodontic appliances, these were quite limited. Thus, orthodontists should be tentative when predicting significant improvement of a patient's profile with this treatment option. Copyright © 2017 American Association of Orthodontists. Published by Elsevier Inc. All rights reserved.
Molecular aspects of zygotic embryogenesis in sunflower (Helianthus annuus L.): correlation of positive histone marks with HaWUS expression and putative link HaWUS/HaL1L.

Science.gov (United States)

Salvini, Mariangela; Fambrini, Marco; Giorgetti, Lucia; Pugliesi, Claudio

2016-01-01

The link HaWUS/ HaL1L , the opposite transcriptional behavior, and the decrease/increase in positive histone marks bond to both genes suggest an inhibitory effect of WUS on HaL1L in sunflower zygotic embryos. In Arabidopsis, a group of transcription factors implicated in the earliest events of embryogenesis is the WUSCHEL-RELATED HOMEOBOX (WOX) protein family including WUSCHEL (WUS) and other 14 WOX protein, some of which contain a conserved WUS-box domain in addition to the homeodomain. WUS transcripts appear very early in embryogenesis, at the 16-cell embryo stage, but gradually become restricted to the center of the developing shoot apical meristem (SAM) primordium and continues to be expressed in cells of the niche/organizing center of SAM and floral meristems to maintain stem cell population. Moreover, WUS has decisive roles in the embryonic program presumably promoting the vegetative-to-embryonic transition and/or maintaining the identity of the embryonic stem cells. However, data on the direct interaction between WUS and key genes for seed development (as LEC1 and L1L) are not collected. The novelty of this report consists in the characterization of Helianthus annuus WUS (HaWUS) gene and in its analysis regarding the pattern of the methylated lysine 4 (K4) of the Histone H3 and of the acetylated histone H3 during the zygotic embryo development. Also, a parallel investigation was performed for HaL1L gene since two copies of the WUS-binding site (WUSATA), previously identified on HaL1L nucleotide sequence, were able to be bound by the HaWUS recombinant protein suggesting a not described effect of HaWUS on HaL1L transcription.
Genome-wide RNA polymerase II profiles and RNA accumulation reveal kinetics of transcription and associated epigenetic changes during diurnal cycles.

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Gwendal Le Martelot

Full Text Available Interactions of cell-autonomous circadian oscillators with diurnal cycles govern the temporal compartmentalization of cell physiology in mammals. To understand the transcriptional and epigenetic basis of diurnal rhythms in mouse liver genome-wide, we generated temporal DNA occupancy profiles by RNA polymerase II (Pol II as well as profiles of the histone modifications H3K4me3 and H3K36me3. We used these data to quantify the relationships of phases and amplitudes between different marks. We found that rhythmic Pol II recruitment at promoters rather than rhythmic transition from paused to productive elongation underlies diurnal gene transcription, a conclusion further supported by modeling. Moreover, Pol II occupancy preceded mRNA accumulation by 3 hours, consistent with mRNA half-lives. Both methylation marks showed that the epigenetic landscape is highly dynamic and globally remodeled during the 24-hour cycle. While promoters of transcribed genes had tri-methylated H3K4 even at their trough activity times, tri-methylation levels reached their peak, on average, 1 hour after Pol II. Meanwhile, rhythms in tri-methylation of H3K36 lagged transcription by 3 hours. Finally, modeling profiles of Pol II occupancy and mRNA accumulation identified three classes of genes: one showing rhythmicity both in transcriptional and mRNA accumulation, a second class with rhythmic transcription but flat mRNA levels, and a third with constant transcription but rhythmic mRNAs. The latter class emphasizes widespread temporally gated posttranscriptional regulation in the mouse liver.
Biomedical potential of chitosan/HA and chitosan/β-1,3-glucan/HA biomaterials as scaffolds for bone regeneration — A comparative study

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Przekora, Agata, E-mail: agata.przekora@umlub.pl [Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodzki 1, 20-093 Lublin (Poland); Palka, Krzysztof [Department of Materials Engineering, Lublin University of Technology, Nadbystrzycka 36, 20-618 Lublin (Poland); Ginalska, Grazyna [Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodzki 1, 20-093 Lublin (Poland)

2016-01-01

The aim of this work was to compare biomedical potential of chitosan/hydroxyapatite (chit/HA) and novel chitosan/β-1,3-glucan/hydroxyapatite (chit/glu/HA) materials as scaffolds for bone regeneration via characterization of their biocompatibility, porosity, mechanical properties, and water uptake behaviour. Biocompatibility of the scaffolds was assessed in direct-contact with the materials using normal human foetal osteoblast cell line. Cytotoxicity and osteoblast proliferation rate were evaluated. Porosity was assessed using computed microtomography analysis and mechanical properties were determined by compression testing. Obtained results demonstrated that chit/HA scaffold possessed significantly better mechanical properties (compressive strength: 1.23 MPa, Young's modulus: 0.46 MPa) than chit/glu/HA material (compressive strength: 0.26 MPa, Young's modulus: 0.25 MPa). However, addition of bacterial β-1,3-glucan to the chit/HA scaffold improved its flexibility and porosity. Moreover, chit/glu/HA scaffold revealed significantly higher water uptake capability (52.6% after 24 h of soaking) compared to the chit/HA (30.7%) and thus can serve as a very good drug delivery carrier. Chit/glu/HA scaffold was also more favourable to osteoblast survival (near 100% viability after 24-h culture), proliferation, and spreading compared to the chit/HA (63% viability). The chit/glu/HA possesses better biomedical potential than chit/HA scaffold. Nevertheless, poor mechanical properties of the chit/glu/HA limit its application to non-load bearing implantation area. - Highlights: • Chitosan/HA and chit/β-1,3-glucan/HA scaffolds for bone regeneration were compared. • Chit/HA significantly reduced osteoblast viability to 63% compared to chit/glu/HA. • Unlike chit/HA, chit/glu/HA favoured cell adhesion, spreading, and proliferation. • Chit/HA had better compressive strength and Young's modulus than chit/glu/HA. • Chit/glu/HA revealed significantly higher
Sequence diversity within the HA-1 gene as detected by melting temperature assay without oligonucleotide probes

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Mattiuz Pier

2005-10-01

Full Text Available Abstract Background The minor histocompatibility antigens (mHags are self-peptides derived from common cellular proteins and presented by MHC class I and II molecules. Disparities in mHags are a potential risk for the development of graft-versus-host disease (GvHD in the recipients of bone marrow from HLA-identical donors. Two alleles have been identified in the mHag HA-1. The correlation between mismatches of the mHag HA-1 and GvHD has been suggested and methods to facilitate large-scale testing were afterwards developed. Methods We used sequence specific primer (SSP PCR and direct sequencing to detect HA-1 gene polymorphisms in a sample of 131 unrelated Italian subjects. We then set up a novel melting temperature (Tm assay that may help identification of HA-1 alleles without oligonucleotide probes. Results We report the frequencies of HA-1 alleles in the Italian population and the presence of an intronic 5 base-pair deletion associated with the immunogeneic allele HA-1H. We also detected novel variable sites with respect to the consensus sequence of HA-1 locus. Even though recombination/gene conversion events are documented, there is considerable linkage disequilibrium in the data. The gametic associations between HA-1R/H alleles and the intronic 5-bp ins/del polymorphism prompted us to try the Tm analysis with SYBR® Green I. We show that the addition of dimethylsulfoxide (DMSO during the assay yields distinct patterns when amplicons from HA-1H homozygotes, HA-1R homozygotes, and heterozygotes are analysed. Conclusion The possibility to use SYBR® Green I to detect Tm differences between allelic variants is attractive but requires great caution. We succeeded in allele discrimination of the HA-1 locus using a relatively short (101 bp amplicon, only in the presence of DMSO. We believe that, at least in certain assets, Tm assays may benefit by the addition of DMSO or other agents affecting DNA strand conformation and stability.
Osteoblast interaction with laser cladded HA and SiO2-HA coatings on Ti-6Al-4V

International Nuclear Information System (INIS)

Yang Yuling; Serpersu, Kaan; He Wei; Paital, Sameer R.; Dahotre, Narendra B.

2011-01-01

In order to improve the bioactivity and biocompatibility of titanium endosseous implants, the morphology and composition of the surfaces were modified. Polished Ti-6Al-4V substrates were coated by a laser cladding process with different precursors: 100 wt.% HA and 25 wt.% SiO 2 -HA. X-ray diffraction of the laser processed samples showed the presence of CaTiO 3 , Ca 3 (PO 4 ) 2 , and Ca 2 SiO 4 phases within the coatings. From in vitro studies, it was observed that compared to the unmodified substrate all laser cladded samples presented improved cellular interactions and bioactivity. The samples processed with 25 wt.% SiO 2 -HA precursor showed a significantly higher HA precipitation after immersion in simulated body fluid than 100 wt.% HA precursor and titanium substrates. The in vitro biocompatibility of the laser cladded coatings and titanium substrate was investigated by culturing of mouse MC3T3-E1 pre-osteoblast cell line and analyzing the cell viability, cell proliferation, and cell morphology. A significantly higher cell attachment and proliferation rate were observed for both laser cladded 100 wt.% HA and 25 wt.% SiO 2 -HA samples. Compared to 100 wt.% HA sample, 25 wt.% SiO 2 -HA samples presented a slightly improved cellular interaction due to the addition of SiO 2 . The staining of the actin filaments showed that the laser cladded samples induced a normal cytoskeleton and well-developed focal adhesion contacts. Scanning electron microscopic image of the cell cultured samples revealed better cell attachment and spreading for 25 wt.% SiO 2 -HA and 100 wt.% HA coatings than titanium substrate. These results suggest that the laser cladding process improves the bioactivity and biocompatibility of titanium. The observed biological improvements are mainly due to the coating induced changes in surface chemistry and surface morphology. Highlights: → Laser cladding of Ti alloys with bioceramics creates new phases. → Laser cladded samples with SiO 2 -doped
Characterization of New PEEK/HA Composites with 3D HA Network Fabricated by Extrusion Freeforming.

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Vaezi, Mohammad; Black, Cameron; Gibbs, David M R; Oreffo, Richard O C; Brady, Mark; Moshrefi-Torbati, Mohamed; Yang, Shoufeng

2016-05-26

Addition of bioactive materials such as calcium phosphates or Bioglass, and incorporation of porosity into polyetheretherketone (PEEK) has been identified as an effective approach to improve bone-implant interfaces and osseointegration of PEEK-based devices. In this paper, a novel production technique based on the extrusion freeforming method is proposed that yields a bioactive PEEK/hydroxyapatite (PEEK/HA) composite with a unique configuration in which the bioactive phase (i.e., HA) distribution is computer-controlled within a PEEK matrix. The 100% interconnectivity of the HA network in the biocomposite confers an advantage over alternative forms of other microstructural configurations. Moreover, the technique can be employed to produce porous PEEK structures with controlled pore size and distribution, facilitating greater cellular infiltration and biological integration of PEEK composites within patient tissue. The results of unconfined, uniaxial compressive tests on these new PEEK/HA biocomposites with 40% HA under both static and cyclic mode were promising, showing the composites possess yield and compressive strength within the range of human cortical bone suitable for load bearing applications. In addition, preliminary evidence supporting initial biological safety of the new technique developed is demonstrated in this paper. Sufficient cell attachment, sustained viability in contact with the sample over a seven-day period, evidence of cell bridging and matrix deposition all confirmed excellent biocompatibility.
Characterization of New PEEK/HA Composites with 3D HA Network Fabricated by Extrusion Freeforming

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Mohammad Vaezi

2016-05-01

Full Text Available Addition of bioactive materials such as calcium phosphates or Bioglass, and incorporation of porosity into polyetheretherketone (PEEK has been identified as an effective approach to improve bone-implant interfaces and osseointegration of PEEK-based devices. In this paper, a novel production technique based on the extrusion freeforming method is proposed that yields a bioactive PEEK/hydroxyapatite (PEEK/HA composite with a unique configuration in which the bioactive phase (i.e., HA distribution is computer-controlled within a PEEK matrix. The 100% interconnectivity of the HA network in the biocomposite confers an advantage over alternative forms of other microstructural configurations. Moreover, the technique can be employed to produce porous PEEK structures with controlled pore size and distribution, facilitating greater cellular infiltration and biological integration of PEEK composites within patient tissue. The results of unconfined, uniaxial compressive tests on these new PEEK/HA biocomposites with 40% HA under both static and cyclic mode were promising, showing the composites possess yield and compressive strength within the range of human cortical bone suitable for load bearing applications. In addition, preliminary evidence supporting initial biological safety of the new technique developed is demonstrated in this paper. Sufficient cell attachment, sustained viability in contact with the sample over a seven-day period, evidence of cell bridging and matrix deposition all confirmed excellent biocompatibility.
Association of Cross Linked C-Telopeptide II Collagen and Hyaluronic Acid with Knee Osteoarthritis Severity

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John Butar Butar

2013-12-01

Full Text Available BACKGROUND: This study was carried out to investigate the association of Cross Linked C-Telopeptide Type I & II Collagen (CTX-I and II and hyaluronic acid (HA with knee osteoarthritis (OA severity. METHODS: Sixty menopause women with primary knee OA were enrolled in this study during their visits to the Outpatient Department. Patients with knee pain during weight bearing, active or passive range of motion, or tenderness with Kellgren-Lawrence (KL grade of more than I were included. Patients with injury, inflammatory and metabolic diseases were excluded. Patients were put in a 10-hour fasting prior to withdrawal of morning blood samples for examinations of HA, CTX-I, interleukin 1 beta (IL-1β, and high sensitivity C reactive protein (hs-CRP level. Second void morning urine specimens were taken for CTXII assessment. HA, CTX-I and II levels were measured by enzyme-linked immunosorbent assay. RESULTS: Sixty menopausal female patients were included in this study, 35 with KL grade II, 17 grade III, and 8 grade IV. Means of CTX-II were significantly different between subjects KL grade IV and III (p=0.021. Correlation of KL grade was significant with CTX-II (p=0.001, r=0.412 and HA (p=0.0411, r=0.269. KL grades were not significantly associated with CTX-I (p=0.8364, r=-0.0272; IL-1β (p=0.5773, r=0.0853 and hs-CRP (p=0.2625, r=0.1470. CONCLUSIONS: CTX-II and HA were associated with severity of knee OA, suggesting that CTX-II and HA can be used as marker for knee OA severity. KEYWORDS: CTX-II, hyaluronic acid, otestoarthritis, knee.
Validation of the Health-Promoting Lifestyle Profile II for Hispanic male truck drivers in the Southwest.

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Mullins, Iris L; O'Day, Trish; Kan, Tsz Yin

2013-08-01

The aims of the study were to validate the English and Spanish Versions of the Health-Promoting Lifestyle Profile II (HPLP II) with Hispanic male truck drivers and to determine if there were any differences in drivers' responses based on driving responsibility. The methods included a descriptive correlation design, the HPLP II (English and Spanish versions), and a demographic questionnaire. Fifty-two Hispanic drivers participated in the study. There were no significant differences in long haul and short haul drivers' responses to the HPLP II. Cronbach's alpha for the Spanish version was .97 and the subscales alphas ranged from .74 to .94. The English version alpha was .92 and the subscales ranged from .68 to .84. Findings suggest the subscales of Health Responsibility, Physical Activities, Nutrition, and Spirituality Growth on the HPLP II Spanish and English versions may not adequately assess health-promoting behaviors and cultural influences for the Hispanic male population in the southwestern border region.
Characterization, Corrosion Resistance, and Cell Response of High-Velocity Flame-Sprayed HA and HA/TiO2 Coatings on 316L SS

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Singh, Tejinder Pal; Singh, Harpreet; Singh, Hazoor

2012-09-01

The main aim of this study is to evaluate corrosion and biocompatibility behavior of thermal spray hydroxyapatite (HA) and hydroxyapatite/titania bond (HA/TiO2)-coated 316L stainless steel (316L SS). In HA/TiO2 coatings, TiO2 was used as a bond coat between HA top coat and 316L SS substrate. The coatings were characterized by x-ray diffraction and scanning electron microscopy/energy dispersive spectroscopy, and corrosion resistance determined for the uncoated substrate and the two coatings. The biological behavior was investigated by the cell culture studies using osteosarcoma cell line KHOS-NP (R-970-5). The corrosion resistance of the steel was found to increase after the deposition of the HA and HA/TiO2 bond coatings. Both HA, as well as, HA/TiO2 coatings exhibit excellent bond strength of 49 and 47 MPa, respectively. The cell culture studies showed that HA-coated 316L SS specimens appeared more biocompatible than the uncoated and HA/TiO2-coated 316L SS specimens.
Osteoblast interaction with laser cladded HA and SiO{sub 2}-HA coatings on Ti-6Al-4V

Energy Technology Data Exchange (ETDEWEB)

Yang Yuling [Department of Physics, Northeastern University, Shenyang 110004 (China); Department of Materials Science and Engineering, University of Tennessee, Knoxville, TN 37996 (United States); Serpersu, Kaan [Department of Materials Science and Engineering, University of Tennessee, Knoxville, TN 37996 (United States); He Wei, E-mail: whe5@utk.edu [Department of Materials Science and Engineering, University of Tennessee, Knoxville, TN 37996 (United States); Department of Mechanical, Aerospace and Biomedical Engineering, University of Tennessee, Knoxville, TN 37996 (United States); Paital, Sameer R. [Department of Materials Science and Engineering, University of Tennessee, Knoxville, TN 37996 (United States); Dahotre, Narendra B. [Department of Materials Science and Engineering, University of North Texas, Denton, TX 76207 (United States)

2011-12-01

In order to improve the bioactivity and biocompatibility of titanium endosseous implants, the morphology and composition of the surfaces were modified. Polished Ti-6Al-4V substrates were coated by a laser cladding process with different precursors: 100 wt.% HA and 25 wt.% SiO{sub 2}-HA. X-ray diffraction of the laser processed samples showed the presence of CaTiO{sub 3}, Ca{sub 3}(PO{sub 4}){sub 2}, and Ca{sub 2}SiO{sub 4} phases within the coatings. From in vitro studies, it was observed that compared to the unmodified substrate all laser cladded samples presented improved cellular interactions and bioactivity. The samples processed with 25 wt.% SiO{sub 2}-HA precursor showed a significantly higher HA precipitation after immersion in simulated body fluid than 100 wt.% HA precursor and titanium substrates. The in vitro biocompatibility of the laser cladded coatings and titanium substrate was investigated by culturing of mouse MC3T3-E1 pre-osteoblast cell line and analyzing the cell viability, cell proliferation, and cell morphology. A significantly higher cell attachment and proliferation rate were observed for both laser cladded 100 wt.% HA and 25 wt.% SiO{sub 2}-HA samples. Compared to 100 wt.% HA sample, 25 wt.% SiO{sub 2}-HA samples presented a slightly improved cellular interaction due to the addition of SiO{sub 2}. The staining of the actin filaments showed that the laser cladded samples induced a normal cytoskeleton and well-developed focal adhesion contacts. Scanning electron microscopic image of the cell cultured samples revealed better cell attachment and spreading for 25 wt.% SiO{sub 2}-HA and 100 wt.% HA coatings than titanium substrate. These results suggest that the laser cladding process improves the bioactivity and biocompatibility of titanium. The observed biological improvements are mainly due to the coating induced changes in surface chemistry and surface morphology. Highlights: {yields} Laser cladding of Ti alloys with bioceramics creates new
Effect of Water-Glass Coating on HA and HA-TCP Samples for MSCs Adhesion, Proliferation, and Differentiation

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Indu Bajpai

2016-01-01

Full Text Available Ca-P and silicon based materials have become very popular as bone tissue engineering materials. In this study, water-glass (also known as sodium silicate glass was coated on sintered hydroxyapatite (HA and HA-TCP (TCP stands for tricalcium phosphate samples and subsequently heat-treated at 600°C for 2 hrs. X-rays diffraction showed the presence of β- and α-TCP phases along with HA in the HA-TCP samples. Samples without coating, with water-glass coating, and heat-treated after water-glass coating were used to observe the adhesion and proliferation response of bone marrow derived-mesenchymal stem cells (MSCs. Cell culture was carried out for 4 hrs, 1 day, and 7 days. Interestingly, all samples showed similar response for cell adhesion and proliferation up to 7-day culture but fibronectin, E-cadherin, and osteogenic differentiation related genes (osteocalcin and osteopontin were significantly induced in heat-treated water-glass coated HA-TCP samples. A water-glass coating on Ca-P samples was not found to influence the cell proliferation response significantly but activated some extracellular matrix genes and induced osteogenic differentiation in the MSCs.
Evaluation of nanohydroxyapaptite (nano-HA) coated epigallocatechin-3-gallate (EGCG) cross-linked collagen membranes.

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Chu, Chenyu; Deng, Jia; Man, Yi; Qu, Yili

2017-09-01

Collagen is the main component of extracellular matrix (ECM) with desirable biological activities and low antigenicity. Collagen materials have been widely utilized in guided bone regeneration (GBR) surgery due to its abilities to maintain space for hard tissue growth. However, pure collagen lacks optimal mechanical properties. In our previous study, epigallocatechin-3-gallate (EGCG) cross-linked collagen membranes, with better biological activities and enhanced mechanical properties, may promote osteoblast proliferation, but their effect on osteoblast differentiation is not very significant. Nanohydroxyapatite (nano-HA) is the main component of mineral bone, which possesses exceptional bioactivity properties including good biocompatibility, high osteoconductivity and osteoinductivity, non-immunogenicity and non-inflammatory behavior. Herein, by analyzing the physical and chemical properties as well as the effects on promoting bone regeneration, we have attempted to present a novel EGCG-modified collagen membrane with nano-HA coating, and have found evidence that the novel collagen membrane may promote bone regeneration with a better surface morphology, without destroying collagen backbone. To evaluate the surface morphologies, chemical and mechanical properties of pure collagen membranes, epigallocatechin-3-gallate (EGCG) cross-linked collagen membranes, nano-HA coated collagen membranes, nano-HA coated EGCG-collagen membranes, (ii) to evaluate the bone regeneration promoted by theses membranes. In the present study, collagen membranes were divided into 4 groups: (1) untreated collagen membranes (2) EGCG cross-linked collagen membranes (3) nano-HA modified collagen membranes (4) nano-HA modified EGCG-collagen membranes. Scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR) were used to evaluate surface morphologies and chemical properties, respectively. Mechanical properties were determined by differential scanning calorimeter (DSC
Püha õhtu seltskonnalaud / Kaire Nurk

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Nurk, Kaire, 1960-

2005-01-01

Püha õhtusöömaaja teemast kunstis. Saksa kunstiajaloolase Horst Schwebeli poolt Leonardo da Vinci "Püha õhtusöömaaja" XX sajandi "töötlustes" välja toodud poliitilise agitatsiooni ja ühisest probleemist haaratud seltskonna kujutiste suunast. Laua kujutisest kunstis
Energy balance of algal biomass production in a 1-ha “Green Wall Panel” plant: How to produce algal biomass in a closed reactor achieving a high Net Energy Ratio

International Nuclear Information System (INIS)

Tredici, M.R.; Bassi, N.; Prussi, M.; Biondi, N.; Rodolfi, L.; Chini Zittelli, G.; Sampietro, G.

2015-01-01

Highlights: • Tetraselmis suecica production in a 1-ha GWP plant in Tuscany (Italy) has a NER < 1. • Major energy costs are embodied energy of GWP and mixing. • In a suitable location (North Africa) the NER increases by 40%. • Integration of photovoltaic in the GWP allows to achieve a NER of 1.7. • T. suecica cultivated in a GWP plant can yield up to 30 t of protein ha −1 year −1 . - Abstract: The annual productivity of Tetraselmis suecica in a 1-ha Green Wall Panel-II (GWP-II) plant in Tuscany (Italy) is 36 t (dry weight) ha −1 year −1 , which corresponds to an energy output of 799 GJ ha −1 year −1 . The energy inputs necessary to attain that productivity amount to 1362 GJ ha −1 year −1 , mainly given by the embodied energy of the reactor (about 30%), mixing (about 40%), fertilizers (11%) and harvesting (10%). The Net Energy Ratio (NER) of T. suecica production is thus 0.6. In a more suitable location (North Africa) productivity nearly doubles, reaching 66 t ha −1 year −1 , but the NER increases only by 40% and the gain (difference between output and inputs) remains negative. In a GWP-II integrated with photovoltaics (PV), the NER becomes 1.7 and the gain surpasses 600 GJ ha −1 year −1 . Marine microalgae cultivation in a GWP plant, in a suitable location, can attain high biomass productivities and protein yields 30 times higher than those achievable with traditional crops (soya). When the GWP reactor is integrated with PV, the process attains a positive energy balance, which substantially enhances its sustainability
Effect of humic acid (HA) on sulfonamide sorption by biochars

International Nuclear Information System (INIS)

Lian, Fei; Sun, Binbin; Chen, Xi; Zhu, Lingyan; Liu, Zhongqi; Xing, Baoshan

2015-01-01

Effect of quantity and fractionation of loaded humic acid (HA) on biochar sorption for sulfonamides was investigated. The HA was applied in two different modes, i.e. pre-coating and co-introduction with sorbate. In pre-coating mode, the polar fractions of HA tended to interact with low-temperature biochars via H-bonding, while the hydrophobic fractions were likely to be adsorbed by high-temperature biochars through hydrophobic and π-π interactions, leading to different composition and structure of the HA adlayers. The influences of HA fractionation on biochar sorption for sulfonamides varied significantly, depending on the nature of interaction between HA fraction and sorbate. Meanwhile, co-introduction of HA with sulfonamides revealed that the effect of HA on sulfonamide sorption was also dependent on HA concentration. These findings suggest that the amount and fractionation of adsorbed HA are tailored by the surface properties of underlying biochars, which differently affect the sorption for organic contaminants. - Highlights: • Effect of quantity and fractionation of coated HA on sorption of sulfonamides by BC was studied. • Fractionation of coated HA is tailored by surface properties of BC. • Roles of HA in BC sorption depend on interaction between HA adlayer and sorbate. • Roles of HA in sulfonamide sorption by BC also depend on HA aqueous concentration. - The quantity and fractionation of adsorbed HA play a major role in sulfonamide sorption by biochars
Imaging of Homeostatic, Neoplastic, and Injured Tissues by HA-Based Probes

Science.gov (United States)

Veiseh, Mandana; Breadner, Daniel; Ma, Jenny; Akentieva, Natalia; Savani, Rashmin C; Harrison, Rene; Mikilus, David; Collis, Lisa; Gustafson, Stefan; Lee, Ting-Yim; Koropatnick, James; Luyt, Leonard G.; Bissell, Mina J.; Turley, Eva A.

2013-01-01

An increase in hyaluronan (HA) synthesis, cellular uptake, and metabolism occurs during the remodeling of tissue microenvironments following injury and during disease processes such as cancer. We hypothesized that multimodality HA-based probes selectively target and detectably accumulate at sites of high HA metabolism, thus providing a flexible imaging strategy for monitoring disease and repair processes. Kinetic analyses confirmed favorable available serum levels of the probe following intravenous (i.v.) or subcutaneous (s.c.) injection. Nuclear (technetium-HA, 99mTc-HA, and iodine-HA, 125I-HA), optical (fluorescent Texas Red-HA, TR-HA), and magnetic resonance (gadolinium-HA, Gd-HA) probes imaged liver (99mTc-HA), breast cancer cells/xenografts (TR-HA, Gd-HA), and vascular injury (125I-HA, TR-HA). Targeting of HA probes to these sites appeared to result from selective HA receptor-dependent localization. Our results suggest that HA-based probes, which do not require polysaccharide backbone modification to achieve favorable half-life and distribution, can detect elevated HA metabolism in homeostatic, injured, and diseased tissues. PMID:22066590
Adsorption of copper(II) on multiwalled carbon nanotubes in the absence and presence of humic or fulvic acids

International Nuclear Information System (INIS)

Sheng Guodong; Li Jiaxing; Shao Dadong; Hu Jun; Chen Changlun; Chen Yixue; Wang Xiangke

2010-01-01

The adsorption of Cu(II) on multiwalled carbon nanotubes (MWCNTs) as a function of pH and ionic strength in the absence and presence of humic acid (HA) or fulvic acid (FA) was studied using batch technique. The results indicated that the adsorption is strongly dependent on pH but independent of ionic strength. A positive effect of HA/FA on Cu(II) adsorption was found at pH 7.5. The adsorption isotherms can be described better by the Freundlich model than by the Langmuir model in the absence and presence of HA/FA. Adsorption isotherms of Cu(II) at higher initial HA/FA concentrations are higher than those of Cu(II) at lower FA/HA concentrations. The thermodynamic data calculated from temperature-dependent adsorption isotherms suggested that the adsorption was spontaneous and enhanced at higher temperature. Results of this work suggest that MWCNTs may be a promising candidate for the removal of heavy metal ions from aqueous solutions.

Solvent extraction of Cs(I), Zn(II), Eu(III) and Am(III) by 2-heptyl-2-methyl-nonanoic acid (Ha) from nitrate solutions

International Nuclear Information System (INIS)

Ramadan, A.; Elnaggar, H.A.; Souka, N.; Abdelfattah, A.

1994-01-01

The present work is devoted to study the extraction behavior of caesium, zinc, europium and americium radioactive isotopes using a new extractant C H 3 (C H 2 ) 6 . C(C 7 H 1 5) (C H 3 ). COOH (Ha) dissolved in benzene from aqueous nitrate media of constant ionic strength (0.1 M) using HNO 3 -Na NO 3 mixtures. The effect temperature on the extraction of these elements was also investigated to evaluate the thermodynamic functions as well as the equilibrium constant of each reaction. The extraction mechanisms were postulated on the light of the available data and the extracted species were formulated in the proposed stoichiometric equation for the extraction of each element individually and these species were suggested to be Cs (A); Zn OH(A) and Zn(A) 2 ; Eu No 3 (A) 2 and Am(A) 3 and for Cs(I), Zn(II), Eu(III) and Am(III), respectively. The thermodynamic parameters show exothermic enthalpy for all the reactions and negative entropy values reflecting very good ordering extraction mechanisms. 10 figs, 1 tab
APOA II genotypes frequency and their interaction with saturated fatty acids consumption on lipid profile of patients with type 2 diabetes.

Science.gov (United States)

Noorshahi, Neda; Sotoudeh, Gity; Djalali, Mahmoud; Eshraghian, Mohamad Reza; Keramatipour, Mohammad; Basiri, Marjan Ghane; Doostan, Farideh; Koohdani, Fariba

2016-08-01

Several studies have suggested that APOA II-265T/C polymorphism affect lipid profile. The aim of this study was to investigate the effect of -265T/C APOA II polymorphism and saturated fatty acids (SFA) intake interaction on lipid profile in diabetic population who are at risk for lipid disorders. In this cross sectional study, 697 type 2 diabetic patients participated. Food consumption data were collected using validated semi-quantitative FFQ during the last year. Realtime-PCR was used to determine APOA II-265T/C genotypes. The interaction between the genotypes and SFA intake with lipid profile was tested using analysis of covariance (ANCOVA). According to APOA II-265T/C (rs5082) genotype distribution results, CC genotype with a frequency of 12.9% and TC with that of 47.7% showed the lowest and highest frequency in our population, respectively. CC genotype subjects had significantly lower total cholesterol, triglyceride, Cholesterol/HDL-c ratio and non-HDL cholesterol than T allele carriers (p = 0.009, p = 0.02, p = 0.02 and p = 0.002, respectively). The interaction between genotype and SFA intake contributed to significant higher levels of LDL-c and LDL/HDL in CCs (p = 0.05 and p = 0.01), suggesting vulnerability of these individuals to high intake of SFA in the diet. APOA II polymorphism may influence the saturated fatty acid intake required to prevent dyslipidemia in the type 2 diabetic population. Copyright © 2015 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.
The profile of people participating in the II International Rally Lovers Narrow Gauge Railway

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Sylwia Barwińska

2016-10-01

Full Text Available The aim of the research was to identify the profile of people participating in the II International Rally Lovers Narrow Gauge Railway. The study was also intended to check the status of tourism development and the development of information technology, which affect the definition of new ways of advertising. The study was conducted using a questionnaire interview. The research tool was the original questionnaire in the printed version. The research was anonymous and preceded by a pilot study
In Vitro Degradation of PHBV Scaffolds and nHA/PHBV Composite Scaffolds Containing Hydroxyapatite Nanoparticles for Bone Tissue Engineering

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Naznin Sultana

2012-01-01

Full Text Available This paper investigated the long-term in vitro degradation properties of scaffolds based on biodegradable polymers and osteoconductive bioceramic/polymer composite materials for the application of bone tissue engineering. The three-dimensional porous scaffolds were fabricated using emulsion-freezing/freeze-drying technique using poly(hydroxybutyrate-co-hydroxyvalerate (PHBV which is a natural biodegradable and biocompatible polymer. Nanosized hydroxyapatite (nHA particles were successfully incorporated into the PHBV scaffolds to render the scaffolds osteoconductive. The PHBV and nHA/PHBV scaffolds were systematically evaluated using various techniques in terms of mechanical strength, porosity, porous morphology, and in vitro degradation. PHBV and nHA/PHBV scaffolds degraded over time in phosphate-buffered saline at 37°C. PHBV polymer scaffolds exhibited slow molecular weight loss and weight loss in the in vitro physiological environment. Accelerated weight loss was observed in nHA incorporated PHBV composite scaffolds. An increasing trend of crystallinity was observed during the initial period of degradation time. The compressive properties decreased more than 40% after 5-month in vitro degradation. Together with interconnected pores, high porosity, suitable mechanical properties, and slow degradation profile obtained from long-term degradation studies, the PHBV scaffolds and osteoconductive nHA/PHBV composite scaffolds showed promises for bone tissue engineering application.
Electrospun Porous PDLLA Fiber Membrane Coated with nHA

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Linhui Qiang

2018-05-01

Full Text Available Porous poly- D, L-lactic acid (PDLLA electrospinning fiber membrane was prepared, and nano-hydroxyapatite (nHA was adsorbed and wrapped into it during the unique shrinking process of the PDLLA fiber membrane to fabricate the PDLLA/nHA composite membrane scaffold for tissue engineering. Compare with the composite fibers prepared by blend electrospinning, most of nHA particles are observed to distribute on the surface of new type composite fibers, which could significantly improve the water wettability and induce the cellular adherence. FTIR analysis indicated that the PDLLA/nHA composite fibrous membrane was formed by physical adsorption. The combination was probed by scanning electron microscope, thermo-gravimetric, water contact angle and mechanical property analysis. It was proved that the nHA particles’ content and distribution, surface wettability, modulus and tensile strength of PDLLA/nHA composite fibrous membrane were influenced by the concentration of nHA dispersion and pores on the PDLLA fiber surface. The 10.6 wt % PDLLA/nHA composite fibrous membrane exhibits a more balanced tensile strength (3.28 MPa and surface wettability (with a water contact angle of 0° of the composite mats. Scanning electron microscope and confocal laser scanning microscopy images of chondrocyte proliferation further showed that the composite scaffold is non-toxic. The adherence and proliferation of chondrocytes on the 10.6 wt % PDLLA/nHA fibrous membrane was significantly improved, compared with PDLLA mat. The 10.6 wt % PDLLA/nHA composite fibrous membrane has potential application value as scaffold material in tissue engineering.
Preparation of bioactive porous HA/PCL composite scaffolds

Energy Technology Data Exchange (ETDEWEB)

Zhao, J.; Guo, L.Y.; Yang, X.B. [Key Laboratory of Advanced Technologies of Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031 (China); Weng, J. [Key Laboratory of Advanced Technologies of Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031 (China)], E-mail: jweng@swjtu.cn

2008-12-30

Porous hydroxyapatite (HA) bioceramic scaffold has been widely attracted the attention to act as a three-dimensional (3D) template for cell adhesion, proliferation, differentiation and thus promoting bone and cartilage regeneration because of its osteoinduction. However, the porous bioceramic scaffold is fragile so that it is not suitable to be applied in clinic for bone repair or replacement. Therefore, it is significant to improve the mechanical property of porous HA bioceramics while the interconnected structure is maintained for tissue ingrowth in vivo. In the present research, a porous composite scaffold composed of HA scaffold and polycaprolactone (PCL) lining was fabricated by the method of polymer impregnating to produce HA scaffold coated with PCL lining. Subsequently, the composite scaffolds were deposited with biomimetic coating for improving the bioactivity. The HA/PCL composite scaffolds with improved mechanical property and bioactivity is expected to be a promising bone substitute in tissue engineering applications.
Preparation of bioactive porous HA/PCL composite scaffolds

International Nuclear Information System (INIS)

Zhao, J.; Guo, L.Y.; Yang, X.B.; Weng, J.

2008-01-01

Porous hydroxyapatite (HA) bioceramic scaffold has been widely attracted the attention to act as a three-dimensional (3D) template for cell adhesion, proliferation, differentiation and thus promoting bone and cartilage regeneration because of its osteoinduction. However, the porous bioceramic scaffold is fragile so that it is not suitable to be applied in clinic for bone repair or replacement. Therefore, it is significant to improve the mechanical property of porous HA bioceramics while the interconnected structure is maintained for tissue ingrowth in vivo. In the present research, a porous composite scaffold composed of HA scaffold and polycaprolactone (PCL) lining was fabricated by the method of polymer impregnating to produce HA scaffold coated with PCL lining. Subsequently, the composite scaffolds were deposited with biomimetic coating for improving the bioactivity. The HA/PCL composite scaffolds with improved mechanical property and bioactivity is expected to be a promising bone substitute in tissue engineering applications
Adiós al Vaticano II? Tres superaciones del Concilio Vaticano II

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José María Vigil

2009-05-01

Full Text Available Resumen El autor confiesa que pertenece a la generación que ha dedicado su vida a implementar la herencia del Concilio Vaticano II, generación que ha tenido a ese concilio como el punto de referencia más importante - eclesialmente hablando - en los últimos 40 años. Sin embargo, aventura la hipótesis de que la problemática del Vaticano II ha quedado ya obsoleta, y lo justifica presentando tres olas de nuevos signos de los tiempos que han transformado radicalmente el panorama teológico y pastoral: la teología de la liberación, el pluralismo religioso y la crisis de la religión. El artículo trata de las grandes olas que avalan esa superación: la teología y la espiritualidad de la liberación, la teología del pluralismo religioso y la revisión de paradigma que provoca la actual crisis de la religión. Estas tres "superaciones" del Vaticano II son, pues, las que nos permiten pensar que lo que se dirimió en el Concilio ya no es el punto de referencia principal de la vida de la Iglesia, ni de las necesidades religiosas más urgentes de la Humanidad. Palabras clave: Concilio Vaticano II; Cambio de paradigma; Teología de la liberación; Pluralismo religioso; Crisis de la religión. Abstract The author belongs to the generation that has dedicated its life to implement the heritage of Vatican Council II, and has taken that Council as the most relevant point of reference, in ecclesiastical terms, for the last 40 years. However, he formulates the hypothesis that the problematics of Vatican II has become obsolete, and justifies that viewpoint presenting three new waves that have transformed radically the theological and pastoral panorama: the theology of liberation, religious pluralism and the crisis in religion. The article focuses on the signs that support such surpassing: the theology and spirituality of liberation, the theology of religious pluralism and the review of paradigms that has brought about the current crisis in religion
The distinct temperament profiles of bipolar I, bipolar II and unipolar patients.

Science.gov (United States)

Akiskal, Hagop S; Kilzieh, Nael; Maser, Jack D; Clayton, Paula J; Schettler, Pamela J; Traci Shea, M; Endicott, Jean; Scheftner, William; Hirschfeld, Robert M A; Keller, Martin B

2006-05-01

Despite a plethora of studies, controversies abound on whether the long-term traits of unipolar and bipolar patients could be differentiated by temperament and whether these traits, in turn, could be distinguished from subthreshold affective symptomatology. 98 bipolar I (BP-I), 64 bipolar II (BP-II), and 251 unipolar major depressive disorder (UP-MDD) patients all when recovered from discrete affective episodes) and 617 relatives, spouses or acquaintances without lifetime RDC diagnoses (the comparison group, CG) were administered a battery of 17 self-rated personality scales chosen for theoretical relevance to mood disorders. Subsamples of each of the four groups also received the General Behavior Inventory (GBI). Of the 436 personality items, 103 that significantly distinguished the three patient groups were subjected to principal components analysis, yielding four factors which reflect the temperamental dimensions of "Mood Lability", "Energy-Assertiveness," "Sensitivity-Brooding," and "Social Anxiety." Most BP-I described themselves as near normal in emotional stability and extroversion; BP-II emerged as labile in mood, energetic and assertive, yet sensitive and brooding; MDD were socially timid, sensitive and brooding. Gender and age did not have marked influence on these overall profiles. Within the MDD group, those with baseline dysthymia were the most pathological (i.e., high in neuroticism, insecurity and introversion). Selected GBI items measuring hypomania and biphasic mood changes were endorsed significantly more often by BP-II. Finally, it is relevant to highlight a methodologic finding about the precision these derived temperament factors brought to the UP-BP differentiation. Unlike BP-I who were low on neuroticism, both BP-II and UP scored high on this measure: yet, in the case of BP-II high neuroticism was largely due to mood lability, in UP it reflected subdepressive traits. We used self-rated personality measures, a possible limitation generic to
Preparation and characterization of bio-composite PEEK/nHA

Science.gov (United States)

Jin, Y. S.; Bian, C. C.; Zhang, Z. Q.; Zhao, Y.; Yang, L.

2017-01-01

PEEK/nHA composite material, with excellent mechanical property as polyetheretherketone (PEEK) and biological activity as hydroxyapatite (HA), has attracted wide attention of medical experts and materials science experts. The addition of hydroxyapatite was the decisive factor for biological activity in PEEK/nHA composite. In this paper, acicular nanohydroxyapatite was prepared by chemical precipitation method with Ca(NO3)2, (NH4)2HPO4 as raw material; PEEK/nHA composite was prepared by solution blending and vacuum sintering method. The composite was characterized with FT-IR, XRD, DSC, TG and mechanical property test. Results showed that the composite has good thermal stability and compressive property when the mass ratio of PEEK to nHA is 10:3; and high nHA content can improve the biological activity of the composite, which can meet the basic requirements for bone tissue engineering scaffold.
Immunomodulatory Effects of Hemagglutinin- (HA- Modified A20 B-Cell Lymphoma Expanded as a Brain Tumor on Adoptively Transferred HA-Specific CD4+ T Cells

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Valentin P. Shichkin

2014-01-01

Full Text Available Previously, the mouse A20 B-cell lymphoma engineered to express hemagglutinin (HA antigen (A20HA was used as a systemic tumor model. In this work, we used the A20HA cells as a brain tumor. HA-specific CD4+ T cells were transferred intravenously in a tail vein 5 days after A20HA intracranial inoculation and analyzed on days 2, 9, and 16 after the adoptive transfer by different methods. The transferred cells demonstrated state of activation as early as day 2 after the adoptive transfer and most the of viable HA-specific cells became anergic on day 16. Additionally, symptoms of systemic immunosuppression were observed in mice with massive brain tumors at a late stage of the brain tumor progression (days 20–24 after the A20HA inoculation. Despite that, a deal of HA-specific CD4+ T cells kept the functional activity even at the late stage of A20HA tumor growth. The activated HA-specific CD4+ T cells were found also in the brain of brain-tumor-bearing mice. These cells were still responding to reactivation with HA-peptide in vitro. Our data support an idea about sufficient role of both the tumor-specific and -nonspecific mechanisms inducing immunosuppression in cancer patients.
Laser treatment of plasma sprayed HA coatings

NARCIS (Netherlands)

Khor, KA; Vreeling, A; Dong, ZL; Cheang, P

1999-01-01

Laser treatment was conducted on plasma sprayed hydroxyapatite (HA) coatings using a Nd-YAG pulse laser. Various laser parameters were investigated. The results showed that the HA surface melted when an energy level of greater than or equal to 2 J and a spot size of 2 mm was employed during
Hyaluronan (HA) interacting proteins RHAMM and hyaluronidase impact prostate cancer cell behavior and invadopodia formation in 3D HA-based hydrogels.

Science.gov (United States)

Gurski, Lisa A; Xu, Xian; Labrada, Lyana N; Nguyen, Ngoc T; Xiao, Longxi; van Golen, Kenneth L; Jia, Xinqiao; Farach-Carson, Mary C

2012-01-01

To study the individual functions of hyaluronan interacting proteins in prostate cancer (PCa) motility through connective tissues, we developed a novel three-dimensional (3D) hyaluronic acid (HA) hydrogel assay that provides a flexible, quantifiable, and physiologically relevant alternative to current methods. Invasion in this system reflects the prevalence of HA in connective tissues and its role in the promotion of cancer cell motility and tissue invasion, making the system ideal to study invasion through bone marrow or other HA-rich connective tissues. The bio-compatible cross-linking process we used allows for direct encapsulation of cancer cells within the gel where they adopt a distinct, cluster-like morphology. Metastatic PCa cells in these hydrogels develop fingerlike structures, "invadopodia", consistent with their invasive properties. The number of invadopodia, as well as cluster size, shape, and convergence, can provide a quantifiable measure of invasive potential. Among candidate hyaluronan interacting proteins that could be responsible for the behavior we observed, we found that culture in the HA hydrogel triggers invasive PCa cells to differentially express and localize receptor for hyaluronan mediated motility (RHAMM)/CD168 which, in the absence of CD44, appears to contribute to PCa motility and invasion by interacting with the HA hydrogel components. PCa cell invasion through the HA hydrogel also was found to depend on the activity of hyaluronidases. Studies shown here reveal that while hyaluronidase activity is necessary for invadopodia and inter-connecting cluster formation, activity alone is not sufficient for acquisition of invasiveness to occur. We therefore suggest that development of invasive behavior in 3D HA-based systems requires development of additional cellular features, such as activation of motility associated pathways that regulate formation of invadopodia. Thus, we report development of a 3D system amenable to dissection of
Sterilisation effect study on granular hydroxyapatite (HA)

International Nuclear Information System (INIS)

Saidu, M.F.; Mashita, M.; Khadijah, K.; Fazan, F.; Khalid, K.A.

2004-01-01

Hydroxyapatite (HA) is a calcium phosphate bio ceramic that has been shown by many authors to be biocompatible with bioactive properties. It is widely accepted as the best synthetic material available for surgical use as a bone graft substitute. HA granules produced by Amerce-SIRIM from local materials underwent 5 types of sterilisation techniques with different ageing periods. Samples were tested for chemical and phase composition and microbial contamination before and after being sterilised. From the microbiological tests done, none of the unsterilised positive control yielded a positive culture. Results from X-Ray diffraction studies found that all the sterilisation techniques did not chemically degrade or structurally change the HA granules significantly. (Author)
Transcriptional profiling in human HaCaT keratinocytes in response to kaempferol and identification of potential transcription factors for regulating differential gene expression

Science.gov (United States)

Kang, Byung Young; Lee, Ki-Hwan; Lee, Yong Sung; Hong, Il; Lee, Mi-Ock; Min, Daejin; Chang, Ihseop; Hwang, Jae Sung; Park, Jun Seong; Kim, Duck Hee

2008-01-01

Kaempferol is the major flavonol in green tea and exhibits many biomedically useful properties such as antioxidative, cytoprotective and anti-apoptotic activities. To elucidate its effects on the skin, we investigated the transcriptional profiles of kaempferol-treated HaCaT cells using cDNA microarray analysis and identified 147 transcripts that exhibited significant changes in expression. Of these, 18 were up-regulated and 129 were down-regulated. These transcripts were then classified into 12 categories according to their functional roles: cell adhesion/cytoskeleton, cell cycle, redox homeostasis, immune/defense responses, metabolism, protein biosynthesis/modification, intracellular transport, RNA processing, DNA modification/ replication, regulation of transcription, signal transduction and transport. We then analyzed the promoter sequences of differentially-regulated genes and identified over-represented regulatory sites and candidate transcription factors (TFs) for gene regulation by kaempferol. These included c-REL, SAP-1, Ahr-ARNT, Nrf-2, Elk-1, SPI-B, NF-κB and p65. In addition, we validated the microarray results and promoter analyses using conventional methods such as real-time PCR and ELISA-based transcription factor assay. Our microarray analysis has provided useful information for determining the genetic regulatory network affected by kaempferol, and this approach will be useful for elucidating gene-phytochemical interactions. PMID:18446059
Cobalt toxicity: Chemical and radiological combined effects on HaCaT keratinocyte cell line

International Nuclear Information System (INIS)

Sandre, C.; Moulin, C.; Bresson, C.; Gault, N.; Poncy, J. L.; Lefaix, J. L.

2010-01-01

Cobalt (Co) is an essential trace element well known as a constituent of vitamin B 12 , but different compounds of Co are also described as highly toxic and/or radio-toxic for individuals or the environment. In nuclear power plants, 58 Co and 60 Co are radioactive isotopes of cobalt present as activation products of stable Co and Ni used in alloys. Skin exposure is a current occupational risk in the hard metal and nuclear industries. As biochemical and molecular cobalt-induced toxicological mechanisms are not fully identified, we investigated cobalt toxicity in a model human keratinocyte cell line, HaCaT. In this study, we propose a model to determine the in vitro chemical impact on cell viability of a soluble form of cobalt (CoCl 2 ) with or without gamma-ray doses to mimic contamination by 60 Co, to elucidate the mechanisms of cobalt intracellular chemical and radiological toxicity. Intracellular cobalt concentration was determined after HaCaT cell contamination and chemical toxicity was evaluated in terms of cellular viability and clonogenic survival. We investigated damage to DNA in HaCaT cells by combined treatment with chemical cobalt and a moderate gamma-ray dose. Additive effects of cobalt and irradiation were demonstrated. The underlying mechanism of cobalt toxicity is not clearly established, but our results seem to indicate that the toxicity of Co(II) and of irradiation arises from production of reactive oxygen species. (authors)
Cobalt toxicity: Chemical and radiological combined effects on HaCaT keratinocyte cell line

Energy Technology Data Exchange (ETDEWEB)

Gault, N. [CEA Fontenay aux Roses, DSV/IRCM/SCSR/LRTS, 92265 Fontenay aux Rose (France); Sandre, C.; Moulin, B.; Bresson, C. [CEA, DEN, SECR, Laboratoire de Speciation des Radionucleides et des Molecules, F-91191 Gif-sur-Yvette (France); Poncy, J.L. [CEA Bruyeres Le Chatel, DSV/IRCM/SREIT/LRT, 91680 Bruyeres Le Chatel (France); Lefaix, J.L. [CEA Caen, DSV/IRCM/SRO/LARIA, 14070 Caen (France)

2010-07-01

Cobalt (Co) is an essential trace element well known as a constituent of vitamin B12, but different compounds of Co are also described as highly toxic and/or radio-toxic for individuals or the environment. In nuclear power plants, {sup 58}Co and {sup 60}Co are radioactive isotopes of cobalt present as activation products of stable Co and Ni used in alloys. Skin exposure is a current occupational risk in the hard metal and nuclear industries. As biochemical and molecular cobalt-induced toxicological mechanisms are not fully identified, we investigated cobalt toxicity in a model human keratinocyte cell line, HaCaT. In this study, we propose a model to determine the in vitro chemical impact on cell viability of a soluble form of cobalt (CoCl{sub 2}) with or without {gamma}-ray doses to mimic contamination by {sup 60}Co, to elucidate the mechanisms of cobalt intracellular chemical and radiological toxicity. Intracellular cobalt concentration was determined after HaCaT cell contamination and chemical toxicity was evaluated in terms of cellular viability and clonogenic survival. We investigated damage to DNA in HaCaT cells by combined treatment with chemical cobalt and a moderate {gamma}-ray dose. Additive effects of cobalt and irradiation were demonstrated. The underlying mechanism of cobalt toxicity is not clearly established, but our results seem to indicate that the toxicity of Co(II) and of irradiation arises from production of reactive oxygen species. (authors)
Cobalt toxicity: Chemical and radiological combined effects on HaCaT keratinocyte cell line

Energy Technology Data Exchange (ETDEWEB)

Sandre, C.; Moulin, C.; Bresson, C. [CEA Saclay, DEN, SECR, Lab Speciat Radionucleides and Mol, F-91191 Gif Sur Yvette (France); Gault, N. [CEA Fontenay Roses, DSV IRCM SCSR LRTS, F-92265 Fontenay Aux Roses (France); Poncy, J. L. [CEA Bruyeres Le Chatel, DSV IRCM SREIT LRT, F-91680 Bruyeres Le Chatel (France); Lefaix, J. L. [CEA Caen, DSV IRCM SRO LARIA, F-14070 Caen (France)

2010-07-01

Cobalt (Co) is an essential trace element well known as a constituent of vitamin B{sub 12}, but different compounds of Co are also described as highly toxic and/or radio-toxic for individuals or the environment. In nuclear power plants, {sup 58}Co and {sup 60}Co are radioactive isotopes of cobalt present as activation products of stable Co and Ni used in alloys. Skin exposure is a current occupational risk in the hard metal and nuclear industries. As biochemical and molecular cobalt-induced toxicological mechanisms are not fully identified, we investigated cobalt toxicity in a model human keratinocyte cell line, HaCaT. In this study, we propose a model to determine the in vitro chemical impact on cell viability of a soluble form of cobalt (CoCl{sub 2}) with or without gamma-ray doses to mimic contamination by {sup 60}Co, to elucidate the mechanisms of cobalt intracellular chemical and radiological toxicity. Intracellular cobalt concentration was determined after HaCaT cell contamination and chemical toxicity was evaluated in terms of cellular viability and clonogenic survival. We investigated damage to DNA in HaCaT cells by combined treatment with chemical cobalt and a moderate gamma-ray dose. Additive effects of cobalt and irradiation were demonstrated. The underlying mechanism of cobalt toxicity is not clearly established, but our results seem to indicate that the toxicity of Co(II) and of irradiation arises from production of reactive oxygen species. (authors)
Enantiomers of HA-966 (3-amino-1-hydroxypyrrolid-2-one) exhibit distinct central nervous system effects: (+)-HA-966 is a selective glycine/N-methyl-D-aspartate receptor antagonist, but (-)-HA-966 is a potent gamma-butyrolactone-like sedative

International Nuclear Information System (INIS)

Singh, L.; Donald, A.E.; Foster, A.C.; Hutson, P.H.; Iversen, L.L.; Iversen, S.D.; Kemp, J.A.; Leeson, P.D.; Marshall, G.R.; Oles, R.J.; Priestley, T.; Thorn, L.; Tricklebank, M.D.; Vass, C.A.; Williams, B.J.

1990-01-01

The antagonist effect of ±-3-amino-1-hydroxypyrrolid-2-one (HA-966) at the N-methyl-D-aspartate (NMDA) receptor occurs through a selective interaction with the glycine modulatory site within the receptor complex. When the enantiomers of ±-HA-966 were resolved, the (R)-(+)-enantiomer was found to be a selective glycine/NMDA receptor antagonist, a property that accounts for its anticonvulsant activity in vivo. In contrast, the (S)-(-)-enantiomer was only weakly active as an NMDA-receptor antagonist, but nevertheless it possessed a marked sedative and muscle relaxant action in vivo. In radioligand binding experiments, (+)-HA-966 inhibited strychnine-insensitive [ 3 H]glycine binding to rat cerebral cortex synaptic membranes with an IC 50 of 12.5 μM, whereas (-)-HA-966 had an IC 50 value of 339 μM. In mice, (+)-HA-966 antagonized sound and N-methyl-DL-aspartic acid (NMDLA)-induced seizures. The coadministration of D-serine dose-dependently antagonized the anticonvulsant effect of a submaximal dose of (+)-HA-966 against NMDLA-induced seizures. The sedative/ataxic effect of racemic HA-966 was mainly attributable to the (-)-enantiomer. It is suggested that, as in the case of the sedative γ-butyrolactone, disruption of striatal dopaminergic mechanisms may be responsible for this action
Preparation, optimization and property of PVA-HA/PAA composite hydrogel.

Science.gov (United States)

Chen, Kai; Liu, Jinlong; Yang, Xuehui; Zhang, Dekun

2017-09-01

PVA-HA/PAA composite hydrogel is prepared by freezing-thawing, PEG dehydration and annealing method. Orthogonal design method is used to choose the optimization combination. Results showed that HA and PVA have the maximum effect on water content. PVA and freezing-thawing cycles have the maximum effect on creep resistance and stress relaxation rate of hydrogel. Annealing temperature and freezing-thawing cycles have the maximum effect on compressive elastic modulus of hydrogel. Comparing with the water content and mechanical properties of 16 kinds of combination, PVA-HA/PAA composite hydrogel with freezing-thawing cycles of 3, annealing temperature of 120°C, PVA of 16%, HA of 2%, PAA of 4% has the optimization comprehensive properties. PVA-HA/PAA composite hydrogel has a porous network structure. There are some interactions between PVA, HA and PAA in hydrogel and the properties of hydrogel are strengthened. The annealing treatment improves the crystalline and crosslinking of hydrogel. Therefore, the annealing PVA-HA/PAA composite hydrogel has good thermostability, strength and mechanical properties. It also has good lubrication property and its friction coefficient is relative low. Copyright © 2017 Elsevier B.V. All rights reserved.

Võidupüha 8. mail? / Leonti Kährik

Index Scriptorium Estoniae

Kährik, Leonti

2005-01-01

Eesti peaks väärikalt tähistama võidupüha kui euroopalike demokraatlike väärtuste võitu natsismi ja fašismi üle. Lähtudes 1940. a. juunini Eestis kehtinud vööndiajast, võiks võidupüha tähistada 8. mail
Prospects of HA-Based Universal Influenza Vaccine

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Anwar M. Hashem

2015-01-01

Full Text Available Current influenza vaccines afford substantial protection in humans by inducing strain-specific neutralizing antibodies (Abs. Most of these Abs target highly variable immunodominant epitopes in the globular domain of the viral hemagglutinin (HA. Therefore, current vaccines may not be able to induce heterosubtypic immunity against the divergent influenza subtypes. The identification of broadly neutralizing Abs (BnAbs against influenza HA using recent technological advancements in antibody libraries, hybridoma, and isolation of single Ab-secreting plasma cells has increased the interest in developing a universal influenza vaccine as it could provide life-long protection. While these BnAbs can serve as a source for passive immunotherapy, their identification represents an important step towards the design of such a universal vaccine. This review describes the recent advances and approaches used in the development of universal influenza vaccine based on highly conserved HA regions identified by BnAbs.
Changes in soft tissue profile using functional appliances in the treatment of skeletal class II malocclusion

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Stamenković Zorana

2015-01-01

Full Text Available Introduction. The effects of orthodontic treatment are considered to be successful if the facial harmony is achieved, while the structures of soft tissue profile are in harmony with skeletal structures of neurocranium and viscerocranium. In patients with skeletal distal bite caused by mandibular retrognathism, facial esthetics is disturbed often, in terms of pronounced convexity of the profile and change in the position and relationship of the lips. Objective. The aim of this study was to determine the extent of soft tissue profile changes in patients with skeletal Class II malocclusion treated with three different orthodontic appliances: Fränkel functional regulator type I (FR-I, Balters’ Bionator type I and Hotz appliance. Methods. The study included 60 patients diagnosed with skeletal Class II malocclusion caused by mandibular retrognathism, in the period of early mixed dentition. Each subgroup of 20 patients was treated with a variety of orthodontic appliances. On the lateral cephalogram, before and after treatment, the following parameters were analyzed: T angle, H angle, the height of the upper lip, the position of the upper and lower lip in relation to the esthetic line. Within the statistical analysis the mean, maximum, minimum, standard deviation, coefficient of variation, two-factor analysis of variance with repeated measures and the factor analysis of variance were calculated using ANOVA, Bonferroni test and Student’s t-test. Results. A significant decrease of angles T and H was noticed in the application of FR-I, from 21.60° to 17.15°, and from 16.45° to 13.40° (p<0.001. FR-I decreased the height of the upper lip from 26.15 mm to 25.85 mm, while Hotz appliance and Balters’ Bionator type I increased the height of the upper lip, thereby deteriorating esthetics of the patient. Conclusion. All used orthodontic appliances lead to changes in soft tissue profile in terms of improving facial esthetics, with the most distinctive
HA03 as an Iranian Candidate Concealed Antigen for Vaccination against Hyalomma anatolicum anatolicum: Comparative Structural and In silico Studies

Directory of Open Access Journals (Sweden)

Mohammadi, A.

2013-12-01

Full Text Available In the last decades researchers had focused on developing a vaccine against tick based on protective antigen. Recombinant vaccines based on concealed antigen from Boophilus microplus have been developed in Australia and Cuba by the name of TICKGARD and GAVAC (De La Fuente and Kocan, 2006. Further studies on this antigen have shown some extent of protection against other species (De Vos et al., 2001. In Iran most important species is Hyalomma anatolicum and limited information about its control are available. This paper reports structural and polymorphic analysis of HA03 as an Iranian candidate concealed antigen of H. a. anatolicum deposited in Gen-Bank .(Aghaeipour et al. GQ228820. The comparison between this antigen and other mid gut concealed antigen that their characteristics are available in GenBank showed there are high rate of similarity between them. The HA03 amino acid sequence had a homology of around 89%, 64%, 56% with HA98, BM86, BM95 respectively. Potential of MHC class I and II binding region indicated a considerable variation between BM86 antigen and its efficiency against Iranian H. a. anatolicum. In addition, predicted major of hydrophobisity and similarity in N-glycosylation besides large amount of cystein and seven EGF like regions presented in protein structure revealed that value of HA03 as a new protective antigen and the necessity of the development, BM86 homolog of H. a. anatolicum HA03 based recombinant vaccine.
HemX is required for production of 2-ketogluconate, the predominant organic anion required for inorganic phosphate solubilization by Burkholderia sp. Ha185.

Science.gov (United States)

Hsu, Pei-Chun Lisa; Condron, Leo; O'Callaghan, Maureen; Hurst, Mark R H

2015-12-01

The bacterium Burkholderia sp. Ha185 readily solubilizes inorganic phosphate by releasing the low molecular weight organic anion, 2-ketogluconate. Using random transposon mutagenesis and in silico analysis, a mutation that caused almost complete abolition of phosphate solubilization was located within hemX, which is part of the hem operon. Burkholderia sp. Ha185 HemX is a multidomain protein, predicted to encode a bifunctional uroporphyrinogen-III synthetase/uroporphyrin-III C-methyltransferase, which has not previously been implicated in phosphate solubilization. Complementation of hemX restored the ability of the mutant to solubilize phosphate in both plate and liquid cultures. Based on a combination of organic-anion profiling, quantitative polymerase chain reaction and in silico analyses, hemX was confirmed to be solely responsible for hydroxyapatite solubilization in Burkholderia sp. Ha185. It is proposed that the biosynthesis of a yet to be determined redox cofactor by HemX is the main pathway for generating 2-ketogluconate via a haem-dependent gluconate 2-dehydrogenase in Burkholderia sp. Ha185. © 2015 Society for Applied Microbiology and John Wiley & Sons Ltd.
Comparison of angiotensin II (Ang II) effects in the internal anal sphincter (IAS) and lower esophageal sphincter smooth muscles.

Science.gov (United States)

Rattan, Satish; Fan, Ya-Ping; Puri, Rajinder N

2002-03-22

Studies were performed to compare the actions of Ang II in the internal anal sphincter (IAS) vs. lower esophageal sphincter (LES) smooth muscles in vitro, in opossum and rabbit. Studies also were carried out in isolated smooth muscle cells. In opossum, Ang II produced no discernible effects in the IAS, but did produce a concentration-dependent contraction in the LES. Conversely, in the rabbit, while Ang II caused a modest response in the LES, it caused a significant contraction in the IAS. The contractile responses of Ang II in the opossum LES were mostly resistant to different neurohumoral antagonists but were antagonized by AT1 antagonist losartan. AT2 antagonist PD 123,319, rather than inhibiting, prolonged the contractile action of Ang II. The contractile actions of Ang II in the opossum LES were not modified by the tyrosine kinase inhibitors (genistein and tyrphostin 1 x 10(-6) M) but were partially attenuated by the PKC inhibitor H-7 (1 x 10(-6) M), Ca2+ channel blocker nicardipine (1 x 10(-5) M), Rho kinase inhibitor HA-1077 (1 x 10(-7) M) or p(44/42) MAP kinase inhibitor PD 98059 (5 x 10(-5) M). The combination of HA-1077 and H-7 did not cause an additive attenuation of Ang II responses. Western blot analyses revealed the presence of both AT1 and AT2 receptors. We conclude that Ang lI-induced contraction of sphincteric smooth muscle occurs primarily by the activation of AT1 receptors at the smooth muscle cells and involves multiple pathways, influx of Ca2+, and PKC, Rho kinase and p(44/42) MAP kinase.
Sr-containing hydroxyapatite: morphologies of HA crystals and bioactivity on osteoblast cells

Energy Technology Data Exchange (ETDEWEB)

Aina, Valentina [Department of Chemistry, Università degli Studi di Torino, Via P. Giuria 7, 10125 Torino (Italy); Centre of Excellence NIS (Nanostructured Interfaces and Surface) Università degli Studi di Torino (Italy); INSTM (Italian National Consortium for Materials Science and Technology), UdR Università di Torino (Italy); Bergandi, Loredana, E-mail: loredana.bergandi@unito.it [Department of Oncology, Università degli Studi di Torino, Via Santena 5/bis, 10126 Torino (Italy); Lusvardi, Gigliola; Malavasi, Gianluca [Department of Chemical and Geological Sciences, Università di Modena and Reggio Emilia, Via Campi 183, 41125 Modena (Italy); Imrie, Flora E.; Gibson, Iain R. [School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD (United Kingdom); Cerrato, Giuseppina [Department of Chemistry, Università degli Studi di Torino, Via P. Giuria 7, 10125 Torino (Italy); Centre of Excellence NIS (Nanostructured Interfaces and Surface) Università degli Studi di Torino (Italy); INSTM (Italian National Consortium for Materials Science and Technology), UdR Università di Torino (Italy); Ghigo, Dario [Department of Oncology, Università degli Studi di Torino, Via Santena 5/bis, 10126 Torino (Italy)

2013-04-01

A series of Sr-substituted hydroxyapatites (HA), of general formula Ca{sub (10−x)}Sr{sub x}(PO{sub 4}){sub 6}(OH){sub 2}, where x = 2 and 4, were synthesized by solid state methods and characterized extensively. The reactivity of these materials in cell culture medium was evaluated, and the behavior towards MG-63 osteoblast cells (in terms of cytotoxicity and proliferation assays) was studied. Future in vivo studies will give further insights into the behavior of the materials. A paper by Lagergren et al. (1975), concerning Sr-substituted HA prepared by a solid state method, reports that the presence of Sr in the apatite composition strongly influences the apatite diffraction patterns. Zeglinsky et al. (2012) investigated Sr-substituted HA by ab initio methods and Rietveld analyses and reported changes in the HA unit cell volume and shape due to the Sr addition. To further clarify the role played by the addition of Sr on the physico-chemical properties of these materials we prepared Sr-substituted HA compositions by a solid state method, using different reagents, thermal treatments and a multi-technique approach. Our results indicated that the introduction of Sr at the levels considered here does influence the structure of HA. There is also evidence of a decrease in the crystallinity degree of the materials upon Sr addition. The introduction of increasing amounts of Sr into the HA composition causes a decrease in the specific surface area and an enrichment of Sr-apatite phase at the surface of the samples. Bioactivity tests show that the presence of Sr causes changes in particle size and/or morphology during soaking in MEM solution; on the contrary the morphology of pure HA does not change after 14 days of reaction. The presence of Sr, as Sr-substituted HA and SrCl{sub 2,} in cultures of human MG-63 osteoblasts did not produce any cytotoxic effect. In fact, Sr-substituted HA increased the proliferation of osteoblast cells and enhanced cell differentiation: Sr in
Sr-containing hydroxyapatite: morphologies of HA crystals and bioactivity on osteoblast cells

International Nuclear Information System (INIS)

Aina, Valentina; Bergandi, Loredana; Lusvardi, Gigliola; Malavasi, Gianluca; Imrie, Flora E.; Gibson, Iain R.; Cerrato, Giuseppina; Ghigo, Dario

2013-01-01

A series of Sr-substituted hydroxyapatites (HA), of general formula Ca (10−x) Sr x (PO 4 ) 6 (OH) 2 , where x = 2 and 4, were synthesized by solid state methods and characterized extensively. The reactivity of these materials in cell culture medium was evaluated, and the behavior towards MG-63 osteoblast cells (in terms of cytotoxicity and proliferation assays) was studied. Future in vivo studies will give further insights into the behavior of the materials. A paper by Lagergren et al. (1975), concerning Sr-substituted HA prepared by a solid state method, reports that the presence of Sr in the apatite composition strongly influences the apatite diffraction patterns. Zeglinsky et al. (2012) investigated Sr-substituted HA by ab initio methods and Rietveld analyses and reported changes in the HA unit cell volume and shape due to the Sr addition. To further clarify the role played by the addition of Sr on the physico-chemical properties of these materials we prepared Sr-substituted HA compositions by a solid state method, using different reagents, thermal treatments and a multi-technique approach. Our results indicated that the introduction of Sr at the levels considered here does influence the structure of HA. There is also evidence of a decrease in the crystallinity degree of the materials upon Sr addition. The introduction of increasing amounts of Sr into the HA composition causes a decrease in the specific surface area and an enrichment of Sr-apatite phase at the surface of the samples. Bioactivity tests show that the presence of Sr causes changes in particle size and/or morphology during soaking in MEM solution; on the contrary the morphology of pure HA does not change after 14 days of reaction. The presence of Sr, as Sr-substituted HA and SrCl 2, in cultures of human MG-63 osteoblasts did not produce any cytotoxic effect. In fact, Sr-substituted HA increased the proliferation of osteoblast cells and enhanced cell differentiation: Sr in HA has a positive effect
Expression Profiles of Cellular Retinol-binding Protein, Type II (CRBP II in Erlang Mountainous Chickens

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H. D. Yin

2014-03-01

Full Text Available Cellular retinol-binding protein II (CRBP II belongs to the family of cellular retinol-binding proteins and plays a major role in absorption, transport, and metabolism of vitamin A. In addition, because vitamin A is correlated with reproductive performance, we measured CRBP II mRNA abundance in erlang mountainous chickens by real-time PCR using the relative quantification method. The expression of CRBP II showed a tissue-specific pattern and egg production rate-dependent changes. The expression was very high (p<0.05 in jejunum and liver, intermediate in kidney, ovary, and oviduct, and lowest (p<0.05 in heart, hypothalamus, and pituitary. In the hypothalamus, oviduct, ovary, and pituitary, CRBP II mRNA abundance were correlated to egg production rate, which increased from 12 wk to 32 wk, peaked at 32 wk relative to the other time points, and then decreased from 32 wk to 45 wk. In contrast, the expression of CRBP II mRNA in heart, jejunum, kidney, and liver was not different at any of the ages evaluated in this study. These data may help to understand the genetic basis of vitamin A metabolism, and suggest that CRBP II may be a candidate gene to affect egg production traits in chickens.
ADCC employing an NK cell line (haNK) expressing the high affinity CD16 allele with avelumab, an anti-PD-L1 antibody.

Science.gov (United States)

Jochems, Caroline; Hodge, James W; Fantini, Massimo; Tsang, Kwong Y; Vandeveer, Amanda J; Gulley, James L; Schlom, Jeffrey

2017-08-01

NK-92 cells, and their derivative, designated aNK, were obtained from a patient with non-Hodgkin lymphoma. Prior clinical studies employing adoptively transferred irradiated aNK cells have provided evidence of clinical benefit and an acceptable safety profile. aNK cells have now been engineered to express IL-2 and the high affinity (ha) CD16 allele (designated haNK). Avelumab is a human IgG1 anti-PD-L1 monoclonal antibody, which has shown evidence of clinical activity in a range of human tumors. Prior in vitro studies have shown that avelumab has the ability to mediate antibody-dependent cell-mediated cytotoxicity (ADCC) of human tumor cells when combined with NK cells. In the studies reported here, the ability of avelumab to enhance the lysis of a range of human carcinoma cells by irradiated haNK cells via the ADCC mechanism is demonstrated; this ADCC is shown to be inhibited by anti-CD16 blocking antibody and by concanamycin A, indicating the use of the granzyme/perforin pathway in tumor cell lysis. Studies also show that while NK cells have the ability to lyse aNK or haNK cells, the addition of NK cells to irradiated haNK cells does not inhibit haNK-mediated lysis of human tumor cells, with or without the addition of avelumab. Avelumab-mediated lysis of tumor cells by irradiated haNK cells is also shown to be similar to that of NK cells bearing the V/V Fc receptor high affinity allele. These studies thus provide the rationale for the clinical evaluation of the combined use of avelumab with that of irradiated adoptively transferred haNK cells. © 2017 UICC.
CHARACTERIZATION OF SORBENT PRODUCED THROUGH IMMOBILIZATION OF HUMIC ACID ON CHITOSAN USING GLUTARALDEHYDE AS CROSS-LINKING AGENT AND Pb(II ION AS ACTIVE SITE PROTECTOR

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Uripto Trisno Santoso

2010-12-01

Full Text Available Sorbent produced through immobilization of humic acid (HA on chitosan using glutaraldehyde as cross-linking agent and Pb(II ions as active site protector has been characterized. Active sorption site of HA was protected by reacting HA with Pb(II ion, and the protected-HA was then activated by glutaraldehyde, crosslinked onto chitosan, and deprotected by 0.1 M disodium ethylenediamine tetra-acetic acid (Na2EDTA. The protected-crosslinking method enhanced the content of immobilized-HA and its chemical stability. Based on the FTIR spectra, crosslinking of HA on chitosan probably occurred through a chemical reaction. The sorption capacity of sorbent still remains unchanged after the second regeneration, but some of HA start to be soluble. The latter shows that cross-linking reaction between HA and chitosan is through formation an unstable product. The effectiveness of sorbent regeneration can also be identified by the XRD pattern.
Calcitriol increases Dicer expression and modifies the microRNAs signature in SiHa cervical cancer cells.

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González-Duarte, Ramiro José; Cázares-Ordoñez, Verna; Romero-Córdoba, Sandra; Díaz, Lorenza; Ortíz, Víctor; Freyre-González, Julio Augusto; Hidalgo-Miranda, Alfredo; Larrea, Fernando; Avila, Euclides

2015-08-01

MicroRNAs play important roles in cancer biology. Calcitriol, the hormonal form of vitamin D3, regulates microRNAs expression in tumor cells. In the present study we asked if calcitriol would modify some of the components of the microRNA processing machinery, namely, Drosha and Dicer, in calcitriol-responsive cervical cancer cells. We found that calcitriol treatment did not affect Drosha mRNA; however, it significantly increased Dicer mRNA and protein expression in VDR-positive SiHa and HeLa cells. In VDR-negative C33-A cells, calcitriol had no effect on Dicer mRNA. We also found a vitamin D response element in Dicer promoter that interacts in vitro to vitamin D and retinoid X receptors. To explore the biological plausibility of these results, we asked if calcitriol alters the microRNA expression profile in SiHa cells. Our results revealed that calcitriol regulates the expression of a subset of microRNAs with potential regulatory functions in cancer pathways, such as miR-22, miR-296-3p, and miR-498, which exert tumor-suppressive effects. In summary, the data indicate that in SiHa cells, calcitriol stimulates the expression of Dicer possibly through the vitamin D response element located in its promoter. This may explain the calcitriol-dependent modulation of microRNAs whose target mRNAs are related to anticancer pathways, further adding to the various anticancer mechanisms of calcitriol.
Serotypes and DNA fingerprint profiles of Pasteurella multocida isolated from raptors

Science.gov (United States)

Wilson, M.A.; Duncan, R.M.; Nordholm, G.E.; Berlowski, B.M.

1995-01-01

Pasteurella multocida isolates from 21 raptors were examined by DNA fingerprint profile and serotyping methods. Isolates were obtained from noncaptive birds of prey found in 11 states from November 28, 1979, through February 10, 1993. Nine isolates were from bald eagles, and the remaining isolates were from hawks, falcons, and owls. Seven isolates were members of capsule group A, and 14 were nonencapsulated. One isolate was identified as somatic type 3, and another was type 3,4,7; both had unique HhaI DNA fingerprint profiles. Nineteen isolates expressed somatic type 1 antigen; HhaI profiles of all type 1 isolates were identical to each other and to the HhaI profile of the reference somatic type 1, strain X-73. The 19 type 1 isolates were differentiated by sequential digestion of DNA with HpaII; four HpaII fingerprint profiles were obtained. The HpaII profile of one isolate was identical to the HpaII profile of strain X-73. Incidence of P. multocida somatic type 1 in raptors suggests that this type may be prevalent in other wildlife or wildlife environments.
Sensitization Profile to Allergens in Patients Using Multi-Test II

Science.gov (United States)

Maniglia, Sergio Fabricio; Tsuru, Fernanda Miyoko; Santos, Victor Carvalho dos; Ueda, Denis Massatsugu

2014-01-01

Introduction Medical intervention in allergies has broadened its perspective, also focusing in the quality of life of patients. Patients are instructed, before using pharmacotherapy agents, to avoid the causal agent. Objective This study aims to analyze the sensitization profile of patients with allergic complaints and identify possible characteristics specific to each age group and gender. Methods A descriptive cross-sectional study included data collected from medical records (from Multi-Test II database, Lincoln Diagnostics Inc. Decatur, Illinois) of 1,912 patients who underwent skin prick test from March to October 2013. Patients were organized and analyzed according to gender, age, and results of the allergens subtypes tested. Results The study was composed of 1,912 patients (60% male and 40% female) of ages between 3 and 87 years. Positive tests were more prevalent in quantity and intensity with the mites Dermatophagoides pteronyssinus and Dermatophagoides farinae, each with 60% of the total analyzed. In second place were pollens, especially Dactylis glomerata and Festuca pratensis. Conclusion The female and male sexes were equally atopic. Fungi and epithelia of dog and cat were not considered potential aeroallergens that could cause symptoms. However, mites are common in Paraná, Brazil. Further studies regarding the pollens are needed, as this study result diverged from the literature. PMID:25992129
Antigenic properties of a transport-competent influenza HA/HIV Env chimeric protein

International Nuclear Information System (INIS)

Ye Ling; Sun Yuliang; Lin Jianguo; Bu Zhigao; Wu Qingyang; Jiang, Shibo; Steinhauer, David A.; Compans, Richard W.; Yang Chinglai

2006-01-01

The transmembrane subunit (gp41) of the HIV Env glycoprotein contains conserved neutralizing epitopes which are not well-exposed in wild-type HIV Env proteins. To enhance the exposure of these epitopes, a chimeric protein, HA/gp41, in which the gp41 of HIV-1 89.6 envelope protein was fused to the C-terminus of the HA1 subunit of the influenza HA protein, was constructed. Characterization of protein expression showed that the HA/gp41 chimeric proteins were expressed on cell surfaces and formed trimeric oligomers, as found in the HIV Env as well as influenza HA proteins. In addition, the HA/gp41 chimeric protein expressed on the cell surface can also be cleaved into 2 subunits by trypsin treatment, similar to the influenza HA. Moreover, the HA/gp41 chimeric protein was found to maintain a pre-fusion conformation. Interestingly, the HA/gp41 chimeric proteins on cell surfaces exhibited increased reactivity to monoclonal antibodies against the HIV Env gp41 subunit compared with the HIV-1 envelope protein, including the two broadly neutralizing monoclonal antibodies 2F5 and 4E10. Immunization of mice with a DNA vaccine expressing the HA/gp41 chimeric protein induced antibodies against the HIV gp41 protein and these antibodies exhibit neutralizing activity against infection by an HIV SF162 pseudovirus. These results demonstrate that the construction of such chimeric proteins can provide enhanced exposure of conserved epitopes in the HIV Env gp41 and may represent a novel vaccine design strategy for inducing broadly neutralizing antibodies against HIV
Measurements of plasma profiles using a fast swept Langmuir probe in the VINETA-II magnetic reconnection experiment

Science.gov (United States)

Shesterikov, I.; Von Stechow, A.; Grulke, O.; Stenzel, R.; Klinger, T.

2017-07-01

A fast-swept Langmuir probe capable to be biased at a high voltages has been constructed and successfully operated at the VINETA-II magnetic reconnection experiment. The presented circuit has two main features beneficial for fast transient parameter changes in laboratory experiments as, e.g., plasma guns or magnetic reconnection: the implementation simplicity and the high voltage sweep range. This work presents its design and performance for time-dependent measurements of VINETA-II plasmas. The probe is biased with a sinusoidal voltage at a fixed frequency. Current - voltage characteristics are measured along the falling and rising slopes of the probe bias. The sweep frequency is fsweep= 150 kHz. The spatiotemporal evolution of radial plasma profiles is obtained by evaluation of the probe characteristics. The plasma density measurements agree with those derived from a microwave interferometer, demonstrating the reliability of the measurements. As a model plasma system, a plasma gun discharge with typical pulse times of 60 μ s is chosen.
Temperament and character profiles in bipolar I, bipolar II and major depressive disorder: Impact over illness course, comorbidity pattern and psychopathological features of depression.

Science.gov (United States)

Zaninotto, Leonardo; Souery, Daniel; Calati, Raffaella; Di Nicola, Marco; Montgomery, Stuart; Kasper, Siegfried; Zohar, Joseph; Mendlewicz, Julien; Robert Cloninger, C; Serretti, Alessandro; Janiri, Luigi

2015-09-15

Studies comparing temperament and character traits between patients with mood disorders and healthy individuals have yielded variable results. The Temperament and Character Inventory (TCI) was administered to 101 bipolar I (BP-I), 96 bipolar II (BP-II), 123 major depressive disorder (MDD) patients, and 125 HS. A series of generalized linear models were performed in order to: (a) compare the TCI dimensions across groups; (b) test any effect of the TCI dimensions on clinical features of mood disorders; and (c) detect any association between TCI dimensions and the psychopathological features of a major depressive episode. Demographic and clinical variables were also included in the models as independent variables. Higher Harm Avoidance was found in BP-II and MDD, but not in BP-I. Higher Self-Transcendence was found in BP-I. Our models also showed higher Self-Directedness in HS, either vs MDD or BP-II. No association was found between any TCI dimension and the severity of symptoms. Conversely, a positive association was found between Harm Avoidance and the overall burden of depressive episodes during lifetime. The cross-sectional design and the heterogeneity of the sample may be the main limitations of our study. In general, our sample seems to support the view of a similar profile of temperament and character between MDD and BP-II, characterized by high Harm Avoidance and low Self-Directedness. In contrast, patients with BP-I only exhibit high Self-Transcendence, having a near-normal profile in terms of Harm Avoidance or Self-Directedness. Copyright © 2015 Elsevier B.V. All rights reserved.
Magnetic Shear and Transport in ECRH Discharges of the TJ-II under Ohmic Induction

Energy Technology Data Exchange (ETDEWEB)

Lopez-Bruna, D.; Castejon, F.; Romero, J. A.; Estrada, T.; Medina, F.; Ochando, M.; Lopez-Fraguas, A.; Ascasibar, E.; Herranz, J.; Sanchez, E.; Luna, E. de la; Pastor, I.

2006-07-01

TJ-II is ha heliac type stellarator characterised by high, but almost constant, vacuum rotational transform throughout the confining volume. In ECRH plasmas, moderate induced ohmic currents (negligible heating and modification of the magnetic field nodules) are enough to disregard the bootstrap contribution, which allows us performing a fair calculation of the evolution of the rotational transform. We use the loop voltage diagnostic to estimate the plasma electrical conductivity. Then the evolution of the rotational transform and shear is related to changes in the profiles of electron and thermal diffusivities: negative shear correlates with decreasing diffusivities in the region of steepest density gradient; transport increases toward zero shear but the achieved positive values are too small to draw conclusions. The radial sweeping of lowest order rational magnetic surfaces does not determine the observed trends in transport. (Author)43 refs.
Magnetic Shear and Transport in ECRH Discharges of the TJ-II under Ohmic Induction

International Nuclear Information System (INIS)

Lopez-Bruna, D.; Castejon, F.; Romero, J. A.; Estrada, T.; Medina, F.; Ochando, M.; Lopez-Fraguas, A.; Ascasibar, E.; Herranz, J.; Sanchez, E.; Luna, E. de la; Pastor, I.

2006-01-01

TJ-II is ha heliac type stellarator characterised by high, but almost constant, vacuum rotational transform throughout the confining volume. In ECRH plasmas, moderate induced ohmic currents (negligible heating and modification of the magnetic field nodules) are enough to disregard the bootstrap contribution, which allows us performing a fair calculation of the evolution of the rotational transform. We use the loop voltage diagnostic to estimate the plasma electrical conductivity. Then the evolution of the rotational transform and shear is related to changes in the profiles of electron and thermal diffusivities: negative shear correlates with decreasing diffusivities in the region of steepest density gradient; transport increases toward zero shear but the achieved positive values are too small to draw conclusions. The radial sweeping of lowest order rational magnetic surfaces does not determine the observed trends in transport. (Author)43 refs
PPAR-δ Agonist With Mesenchymal Stem Cells Induces Type II Collagen-Producing Chondrocytes in Human Arthritic Synovial Fluid.

Science.gov (United States)

Heck, Bruce E; Park, Joshua J; Makani, Vishruti; Kim, Eun-Cheol; Kim, Dong Hyun

2017-08-01

Osteoarthritis (OA) is an inflammatory joint disease characterized by degeneration of articular cartilage within synovial joints. An estimated 27 million Americans suffer from OA, and the population is expected to reach 67 million in the United States by 2030. Thus, it is urgent to find an effective treatment for OA. Traditional OA treatments have no disease-modifying effect, while regenerative OA therapies such as autologous chondrocyte implantation show some promise. Nonetheless, current regenerative therapies do not overcome synovial inflammation that suppresses the differentiation of mesenchymal stem cells (MSCs) to chondrocytes and the expression of type II collagen, the major constituent of functional cartilage. We discovered a synergistic combination that overcame synovial inflammation to form type II collagen-producing chondrocytes. The combination consists of peroxisome proliferator-activated receptor (PPAR) δ agonist, human bone marrow (hBM)-derived MSCs, and hyaluronic acid (HA) gel. Interestingly, those individual components showed their own strong enhancing effects on chondrogenesis. GW0742, a PPAR-δ agonist, greatly enhanced MSC chondrogenesis and the expression of type II collagen and glycosaminoglycan (GAG) in hBM-MSC-derived chondrocytes. GW0742 also increased the expression of transforming growth factor β that enhances chondrogenesis and suppresses cartilage fibrillation, ossification, and inflammation. HA gel also increased MSC chondrogenesis and GAG production. However, neither GW0742 nor HA gel could enhance the formation of type II collagen-producing chondrocytes from hBM-MSCs within human OA synovial fluid. Our data demonstrated that the combination of hBM-MSCs, PPAR-δ agonist, and HA gel significantly enhanced the formation of type II collagen-producing chondrocytes within OA synovial fluid from 3 different donors. In other words, the novel combination of PPAR-δ agonist, hBM-MSCs, and HA gel can overcome synovial inflammation to form

Fabrication and evaluation of porous Ti–HA bio-nanomaterial by leaching process

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A.M. Omran

2015-05-01

Full Text Available A porous surface of Ti–HA composite was successfully fabricated by pulsed current activated sintering (PCAS, followed by leaching using diluted H3PO4. The Ti and HA powders were mixed at different contents of the HA, Ti-5, 10, 30 and 40 wt% HA powders. The mixed powders were pressed in a coated graphite die using pulsed current activated sintering (PCAS under pressure of 60 MPa at temperature of 1000 °C for 5 min. The sintered Ti–HA specimens were immersed in the eight kinds of leaching solutions at room temperature for 24 h. The leached specimen’s surfaces were characterized using XRD, SEM, EDX and Rockwell hardness. The XRD patterns after sintering show that many phases were detected at the sintered specimen surfaces such as; Ti2O, CaO, CaTiO3, TixPy in addition to the remaining Ti and HA. Furthermore, the high concentration H3PO4 leaching solution is more efficient than the low concentration. Also the produced porous surfaces of Ti–HA materials containing more than 30% HA have a low relative density and hardness than the commercial Ti–6Al–4V ELI alloy. In a word, the presence of porous surface coated by HA will promote the nucleation of the biological apatite created with the human tissue and increase the bonding between them. So, the produced porous materials are considered so easy for the muscle cells to permeate after transplanted with high coherence.
Severe Extracellular Matrix Abnormalities and Chondrodysplasia in Mice Lacking Collagen Prolyl 4-Hydroxylase Isoenzyme II in Combination with a Reduced Amount of Isoenzyme I.

Science.gov (United States)

Aro, Ellinoora; Salo, Antti M; Khatri, Richa; Finnilä, Mikko; Miinalainen, Ilkka; Sormunen, Raija; Pakkanen, Outi; Holster, Tiina; Soininen, Raija; Prein, Carina; Clausen-Schaumann, Hauke; Aszódi, Attila; Tuukkanen, Juha; Kivirikko, Kari I; Schipani, Ernestina; Myllyharju, Johanna

2015-07-03

Collagen prolyl 4-hydroxylases (C-P4H-I, C-P4H-II, and C-P4H-III) catalyze formation of 4-hydroxyproline residues required to form triple-helical collagen molecules. Vertebrate C-P4Hs are α2β2 tetramers differing in their catalytic α subunits. C-P4H-I is the major isoenzyme in most cells, and inactivation of its catalytic subunit (P4ha1(-/-)) leads to embryonic lethality in mouse, whereas P4ha1(+/-) mice have no abnormalities. To study the role of C-P4H-II, which predominates in chondrocytes, we generated P4ha2(-/-) mice. Surprisingly, they had no apparent phenotypic abnormalities. To assess possible functional complementarity, we established P4ha1(+/-);P4ha2(-/-) mice. They were smaller than their littermates, had moderate chondrodysplasia, and developed kyphosis. A transient inner cell death phenotype was detected in their developing growth plates. The columnar arrangement of proliferative chondrocytes was impaired, the amount of 4-hydroxyproline and the Tm of collagen II were reduced, and the extracellular matrix was softer in the growth plates of newborn P4ha1(+/-);P4ha2(-/-) mice. No signs of uncompensated ER stress were detected in the mutant growth plate chondrocytes. Some of these defects were also found in P4ha2(-/-) mice, although in a much milder form. Our data show that C-P4H-I can to a large extent compensate for the lack of C-P4H-II in proper endochondral bone development, but their combined partial and complete inactivation, respectively, leads to biomechanically impaired extracellular matrix, moderate chondrodysplasia, and kyphosis. Our mouse data suggest that inactivating mutations in human P4HA2 are not likely to lead to skeletal disorders, and a simultaneous decrease in P4HA1 function would most probably be required to generate such a disease phenotype. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.
CILogon-HA. Higher Assurance Federated Identities for DOE Science

Energy Technology Data Exchange (ETDEWEB)

Basney, James [Univ. of Illinois, Urbana-Champaign, IL (United States)

2015-08-01

The CILogon-HA project extended the existing open source CILogon service (initially developed with funding from the National Science Foundation) to provide credentials at multiple levels of assurance to users of DOE facilities for collaborative science. CILogon translates mechanism and policy across higher education and grid trust federations, bridging from the InCommon identity federation (which federates university and DOE lab identities) to the Interoperable Global Trust Federation (which defines standards across the Worldwide LHC Computing Grid, the Open Science Grid, and other cyberinfrastructure). The CILogon-HA project expanded the CILogon service to support over 160 identity providers (including 6 DOE facilities) and 3 internationally accredited certification authorities. To provide continuity of operations upon the end of the CILogon-HA project period, project staff transitioned the CILogon service to operation by XSEDE.
Wear characterization of nano-hydroxyapatite with addition of titanium (HA-Ti)

Science.gov (United States)

Rosmamuhamadani, R.; Arawi, A. Z. O.; Talari, M. K.; Mahat, M. M.; Bonnia, N. N.; Sabrina; Yahaya, M.; Sulaiman, S.; Ismail, M. I. S.

2018-04-01

Hydroxyapatite (Ca10 (PO4)6(OH)2, HA), is an attractive material of an inorganic compound whose chemical composition and crystallographic structures are similar to the composition of the bone. A natural source such as egg shells is composed of 94 wt. % of calcium carbonate (CaCO3), which can be calcined as calcium oxide (CaO) by the calcinations process. The efficient temperature to produce CaO is 900 °C for 2 hours. The synthesis of nano-HA was done by the mixing the diammonium phosphate (DAP) and calcium hydroxide (Ca(OH)2) and subjected into a microwave for 30 minutes at 1100 W irradiation power. Ball milling process was used for 30 minutes to mix the nano-HA with different compositions of titanium. These were pressed to form pallets by hand hydraulic pump (force=2300 psi). The pallets then were sintered at 1200 °C with the heating rate of 3 °C/min for 2 hours. The pallets were tested by several mechanical testing including hardness, compression strength and wear. From the results, HA-25wt. %Ti composite gave the highest hardness, compression and coefficient of friction for wear test values which were 89.6 Hv, 82.5MPa and 0.76μ respectively. It showed that by adding Ti to nano-HA, the mechanical properties of nano-HA could be enhanced. The microstructure analyses by optical micrograph showed that nano-HA-Ti particles displayed shape likes needle morphology. The particles showed the high tendency to form the agglomerations.
Degradation behavior and compatibility of micro, nanoHA/chitosan scaffolds with interconnected spherical macropores.

Science.gov (United States)

Ruixin, Li; Cheng, Xu; Yingjie, Liu; Hao, Li; Caihong, Shi; Weihua, Su; Weining, An; Yinghai, Yuan; Xiaoli, Qin; Yunqiang, Xu; Xizheng, Zhang; Hui, Li

2017-10-01

Hydroxyapatite/Chitosan (HA/CS) composite have significant application in biomedical especially for bone replacement. Inorganic particle shape and size of composite affect the scaffold mechanical property, biological property, and degradation. The aim of this study was to fabricate HA/CS scaffold with good pore connectivity and analyze their biological, degradation properties. Microhydroxyapatite/chitosan (mHA/CS) and nanohydroxyapatite/chitosan (nHA/CS) composite scaffolds with interconnected spherical pore architectures were fabricated. Composite scaffolds structure parameters were analyzed using micro CT. Cell proliferation and morphology were tested and compared between two scaffolds using mouse osteoblastic cell line MC3T3-E1. To research the composite degradation in lysozyme PBS solution, degradation rate and reducing sugar content were tested, and scaffolds morphology were observed by SEM. The results showed that microHA and nanoHA were fabricated by being calcined and synthesis methods, and their infrared spectra are very similar. EDAX composition analysis demonstrated that both of microHA and nanoHA were calcium deficiency HA. Micro-CT results demonstrated the scaffolds had interconnected spherical pores, and the structure parameters were similar. Cell viabilities were significant increased with cultured time, but there were no significant difference between microHA/CS and nanoHA/CS scaffolds. Scaffold structure was gradually destroyed and inorganic composition HA particles are more prominent with degradation time. (1) Inorganic particle shape and size of composite affect the scaffold mechanical property, biological property, and degradation. NanoHA/CS and microHA/CS scaffolds with good pore connectivity were fabricated and their biological, degradation properties were studied in this manuscript. (2) The scaffold with interconnected porosity construct provides the necessary support for cells to proliferate and maintain their differentiated function, and
Microstructure, mechanical properties, and biological response to functionally graded HA coatings

International Nuclear Information System (INIS)

Rabiei, Afsaneh; Blalock, Travis; Thomas, Brent; Cuomo, Jerry; Yang, Y.; Ong, Joo

2007-01-01

Hydroxyapatite (HA) [Ca 10 (PO 4 ) 6 (OH) 2 ] is the primary mineral content, representing 43% by weight, of bone. Applying a thin layer of HA, to the surface of a metal implant, can promote osseointegration and increase the mechanical stability of the implant. In this study, a biocompatible coating comprising an HA film with functionally graded crystallinity is being deposited on a heated substrate in an Ion Beam Assisted Deposition (IBAD) system. The microstructure of the film was studied using Transmission Electron Microscopy techniques. Finally, initial cell adhesion and cell differentiation on the coating was evaluated using ATCC CRL 1486 human embryonic palatal mesenchymal cell, an osteoblast precursor cell line. The results have shown superior mechanical properties and biological response to the functionally graded HA film
Intra-articular Hyaluronic Acid (HA) and Platelet Rich Plasma (PRP) injection versus Hyaluronic acid (HA) injection alone in Patients with Grade III and IV Knee Osteoarthritis (OA): A Retrospective Study on Functional Outcome.

Science.gov (United States)

Saturveithan, C; Premganesh, G; Fakhrizzaki, S; Mahathir, M; Karuna, K; Rauf, K; William, H; Akmal, H; Sivapathasundaram, N; Jaspreet, K

2016-07-01

Introduction: Intra-articular hyaluronic acid (HA) is widely utilized in the treatment of knee osteoarthritis whereas platelet rich plasma (PRP) enhances the regeneration of articular cartilage. This study analyses the efficacy of HA and PRP in grade III and IV knee osteoarthritis. Methodology: This is a cross sectional study with retrospective review of 64 patients (101 knees) which includes 56 knees injected with HA+ PRP, and 45 knees with HA only. Results: During the post six months International Knee Documentation Committee (IKDC) evaluation, HA+PRP group showed marked improvement of 24.33 compared to 12.15 in HA group. Decrement in visual analogue score (VAS) in HA+PRP was 1.9 compared to 0.8 in HA group. Conclusion: We propose intra-articular HA and PRP injections as an optional treatment modality in Grade III and IV knee osteoarthritis in terms of functional outcome and pain control for up to six months when arthroplasty is not an option.
Intra-articular Hyaluronic Acid (HA and Platelet Rich Plasma (PRP injection versus Hyaluronic acid (HA injection alone in Patients with Grade III and IV Knee Osteoarthritis (OA: A Retrospective Study on Functional Outcome

Directory of Open Access Journals (Sweden)

Saturveithan C

2016-07-01

Full Text Available Introduction: Intra-articular hyaluronic acid (HA is widely utilized in the treatment of knee osteoarthritis whereas platelet rich plasma (PRP enhances the regeneration of articular cartilage. This study analyses the efficacy of HA and PRP in grade III and IV knee osteoarthritis. Methodology: This is a cross sectional study with retrospective review of 64 patients (101 knees which includes 56 knees injected with HA+ PRP, and 45 knees with HA only. Results: During the post six months International Knee Documentation Committee (IKDC evaluation, HA+PRP group showed marked improvement of 24.33 compared to 12.15 in HA group. Decrement in visual analogue score (VAS in HA+PRP was 1.9 compared to 0.8 in HA group. Conclusion: We propose intra-articular HA and PRP injections as an optional treatment modality in Grade III and IV knee osteoarthritis in terms of functional outcome and pain control for up to six months when arthroplasty is not an option.
A-Ha. Drum'n'bassi supernimi FABIO

Index Scriptorium Estoniae

2002-01-01

7.sept. annab norra menukaim popansambel A-Ha Tallinnas Lauluväljakul kontserdi, kus presenteerib ka oma viimast albumit "Lifelines". 14. sept.tuleb drum'n'bassi spetsialist Fabio Tallinna üritusele Circulation
Mechanical strength of [HA/Bioplastic/Sericin] composite part printed by bioprinter

Energy Technology Data Exchange (ETDEWEB)

Tontowi, Alva Edy, E-mail: alvaedytontowi@ugm.ac.id; Setiawan, Agris [Department of Mechanical and Industrial Engineering Faculty of Engineering Universitas Gadjah Mada (Indonesia)

2016-06-17

The aim of this research was to determine the effect of hydroxyapatite (HA) content in printed biocomposite to its mechanical strength. The biocomposite paste was prepared by composing HA, bioplastic and sericin with various ratios of [HA/Bioplastic]: 40/60, 50/50, 60,40 and 70/30. Sericin of 0.3% weight was added to the biocomposite. Mechanical test was conducted to observe tensile (ASTM D 638 type 4) and flexural strength (ASTM D 790). Both type of specimens were fabricated using 3D Printer. Printing process parameter (infill speed, print speed and layer height) were set up as 60 mm/s, 10 mm/s, 0.35 mm, respectively. Results showed that biocomposite with [HA/Biplastic]. weight ratio of 60/40(w/w) has an optimum tensile (3.89 ± 1.26 MPa) and flexural strength (2.51 ± 0.45 MPa). Scanning electron microscope observation indicated that microstructure of specimen was influenced by the percentage of the hydroxyapatite. There was no agglomeration of HA particle within the composite.
Mechanical strength of [HA/Bioplastic/Sericin] composite part printed by bioprinter

International Nuclear Information System (INIS)

Tontowi, Alva Edy; Setiawan, Agris

2016-01-01

The aim of this research was to determine the effect of hydroxyapatite (HA) content in printed biocomposite to its mechanical strength. The biocomposite paste was prepared by composing HA, bioplastic and sericin with various ratios of [HA/Bioplastic]: 40/60, 50/50, 60,40 and 70/30. Sericin of 0.3% weight was added to the biocomposite. Mechanical test was conducted to observe tensile (ASTM D 638 type 4) and flexural strength (ASTM D 790). Both type of specimens were fabricated using 3D Printer. Printing process parameter (infill speed, print speed and layer height) were set up as 60 mm/s, 10 mm/s, 0.35 mm, respectively. Results showed that biocomposite with [HA/Biplastic]. weight ratio of 60/40(w/w) has an optimum tensile (3.89 ± 1.26 MPa) and flexural strength (2.51 ± 0.45 MPa). Scanning electron microscope observation indicated that microstructure of specimen was influenced by the percentage of the hydroxyapatite. There was no agglomeration of HA particle within the composite.
New Coll–HA/BT composite materials for hard tissue engineering

Energy Technology Data Exchange (ETDEWEB)

Zanfir, Andrei Vlad [Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Material Science, “Politehnica” University of Bucharest, 1-7 Gh. Polizu Street, RO-011061 Bucharest (Romania); Voicu, Georgeta, E-mail: getav2001@yahoo.co.uk [Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Material Science, “Politehnica” University of Bucharest, 1-7 Gh. Polizu Street, RO-011061 Bucharest (Romania); Busuioc, Cristina; Jinga, Sorin Ion [Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Material Science, “Politehnica” University of Bucharest, 1-7 Gh. Polizu Street, RO-011061 Bucharest (Romania); Albu, Madalina Georgiana [Department of Collagen, Branch of Leather and Footwear Research, National Institute of Research and Development for Textile and Leather, 93 I. Minulescu Street, RO-031215 Bucharest (Romania); Iordache, Florin [Department of Fetal and Adult Stem Cell Therapy, “Nicolae Simionescu” Institute of Cellular Biology and Pathology of Romanian Academy, 8 B.P. Hasdeu Street, RO-050568 Bucharest (Romania)

2016-05-01

The integration of ceramic powders in composite materials for bone scaffolds can improve the osseointegration process. This work was aimed to the synthesis and characterization of new collagen–hydroxyapatite/barium titanate (Coll–HA/BT) composite materials starting from barium titanate (BT) nanopowder, hydroxyapatite (HA) nanopowder and collagen (Coll) gel. BT nanopowder was produced by combining two wet-chemical approaches, sol–gel and hydrothermal methods. The resulting materials were characterized in terms of phase composition and microstructure by X-ray diffraction, Raman spectroscopy, scanning electron microscopy and transmission electron microscopy. Moreover, the biocompatibility and bioactivity of the composite materials were assessed by in vitro tests. The synthesized BT particles exhibit an average size of around 35 nm and a spherical morphology, with a pseudo-cubic or tetragonal symmetry. The diffraction spectra of Coll–HA and Coll–HA/BT composite materials indicate a pronounced interaction between Col and the mineral phases, meaning a good mineralization of Col fibres. As well, the in vitro tests highlight excellent osteoinductive properties for all biological samples, especially for Coll–HA/BT composite materials, fact that can be attributed to the ferromagnetic properties of BT. - Highlights: • Collagen–hydroxyapatite/barium titanate composite materials were synthesized. • Barium titanate was produced by combining the sol–gel and hydrothermal methods. • The in vitro tests highlight excellent osteoinductive properties for all samples.
New Coll–HA/BT composite materials for hard tissue engineering

International Nuclear Information System (INIS)

Zanfir, Andrei Vlad; Voicu, Georgeta; Busuioc, Cristina; Jinga, Sorin Ion; Albu, Madalina Georgiana; Iordache, Florin

2016-01-01

The integration of ceramic powders in composite materials for bone scaffolds can improve the osseointegration process. This work was aimed to the synthesis and characterization of new collagen–hydroxyapatite/barium titanate (Coll–HA/BT) composite materials starting from barium titanate (BT) nanopowder, hydroxyapatite (HA) nanopowder and collagen (Coll) gel. BT nanopowder was produced by combining two wet-chemical approaches, sol–gel and hydrothermal methods. The resulting materials were characterized in terms of phase composition and microstructure by X-ray diffraction, Raman spectroscopy, scanning electron microscopy and transmission electron microscopy. Moreover, the biocompatibility and bioactivity of the composite materials were assessed by in vitro tests. The synthesized BT particles exhibit an average size of around 35 nm and a spherical morphology, with a pseudo-cubic or tetragonal symmetry. The diffraction spectra of Coll–HA and Coll–HA/BT composite materials indicate a pronounced interaction between Col and the mineral phases, meaning a good mineralization of Col fibres. As well, the in vitro tests highlight excellent osteoinductive properties for all biological samples, especially for Coll–HA/BT composite materials, fact that can be attributed to the ferromagnetic properties of BT. - Highlights: • Collagen–hydroxyapatite/barium titanate composite materials were synthesized. • Barium titanate was produced by combining the sol–gel and hydrothermal methods. • The in vitro tests highlight excellent osteoinductive properties for all samples.
Validation of SAGE II ozone measurements

Science.gov (United States)

Cunnold, D. M.; Chu, W. P.; Mccormick, M. P.; Veiga, R. E.; Barnes, R. A.

1989-01-01

Five ozone profiles from the Stratospheric Aerosol and Gas Experiment (SAGE) II are compared with coincident ozonesonde measurements obtained at Natal, Brazil, and Wallops Island, Virginia. It is shown that the mean difference between all of the measurements is about 1 percent and that the agreement is within 7 percent at altitudes between 20 and 53 km. Good agreement is also found for ozone mixing ratios on pressure surfaces. It is concluded that the SAGE II profiles provide useful ozone information up to about 60 km altitude.
CRED Shallow CTD Profiles; Guam; Cruise: HA1101_LEGIII, Data Date Range: 20110504-20110507. (NODC Accession 0107470)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
Human hypervariable sequences in risk assessment: rare Ha-ras alleles in cancer patients

International Nuclear Information System (INIS)

Krontiris, T.G.; DiMartino, N.A.; Mitcheson, H.D.; Lonergan, J.A.; Begg, C.; Parkinson, D.R.

1987-01-01

A variable tandem repeat (VTR) is responsible for the hyperallelism one kilobase 3' to the human c-Ha-ras-1 (Ha-ras) gene. Thirty-two distinct restriction fragments, comprising 3 allelic classes by frequency of occurrence, have thus far been detected in a sample size of approximately 800 caucasians. Rare Ha-ras alleles, 21 in all, are almost exclusively confined to the genomes of cancer patients. From their data the authors have computed the relative cancer risk associated with possession of a rare Ha-ras allele to be 27. To understand the molecular basis for this phenomenon, they have begun to clone Ha-ras fragments from nontumor DNA of cancer patients. They report here the weak activation, as detected by transfection and transformation of NIH 3T3 mouse cells, of two Ha-ras genes which were obtained from lymphocyte DNA of a melanoma patient. They have mapped the regions that confer this transforming activity to the fragment containing the VTR in one Ha-ras clone and the fragment containing gene coding sequences in the other
Poredbena analiza Haških i Roterdamskih pravila

Directory of Open Access Journals (Sweden)

Vesna Skorupan Wolff

2010-12-01

Full Text Available Primjenom komparativne metode uspoređuju se najvažnije konvencijske odredbe i rješenja Roterdamskih pravila s onima sadržanim u Haškim pravilima. Raščlanjuje se većina recentnih odredbi Roterdamskih pravila koje predstavljaju ključne elemente po kojime se ona razlikuju od Haških pravila. U okviru toga ukazuje se na važnost novih pojmova, instituta i osoba koje sudjeluju u prijevoznom poslu. Govori se i o važnim odredbama koje su podudarne jer upravo brojna tradicionalna rješenja koja donose Roterdamska pravila mogu pridonijeti njihovom bržem i lakšem akceptiranju. Roterdamska pravila nastoje na suvremeni način urediti ugovore o prijevozu stvari morem, a pod određenim uvjetima primjenjivat će se i na prijevoze drugim prijevoznim granama. Opsežnost konvencije govori o njezinoj sveobuhvatnosti i velikoj preciznosti u propisivanju gotovo svih pitanja iz područja pravnog uređenja pomorskih prijevoznih ugovora, i više od toga, ona je jedinstven primjer temeljite raščlambe svih obveza i odgovornosti ugovornih stranaka i općenito svih subjekata koji se pojavljuju u prijevoznom poslu. Cilj poredbene analize Haških i Roterdamskih pravila je spoznati što novo donose Roterdamska pravila i procijeniti kako se ona uklapaju u postojeće pravno uređenje prijevoza stvari morem. Istodobno cilj je spoznati koje se odredbe Haških pravila danas izlažu kritici zbog njihove neprilagođenosti suvremenim uvjetima prijevoza i novim i drugačijim odnosima snaga između ugovornih strana u pomorskim prijevoznim ugovorima.
New therapeutic agent for radiation synovectomy - preparation of 166Ho-EDTMP-HA particle

International Nuclear Information System (INIS)

Bai, H.; Jin, X.; Du, J.; Wang, F.; Chen, D.; Fan, H.; Cheng, Z.; Zhang, J.

1997-01-01

In order to prepare new therapeutical agent for radiation synovectomy, Hydroxyapatite (HA) was labelled with 166 Ho by EDTMP that had high affinity to HA particles. Radiolabelling of HA particles was divided into two steps, 166 Ho-EDTMP was prepared first; then mixed with HA particles completely and vibrated for 15 minutes on the micromixer at room temperature, washed 3 times with deionized water. Radiolabelling particle was separated from free 166 Ho via centrifugation to determine its radiolabelling efficiency. 166 Ho-EDTMP-HA and 166 Ho-EDTMP were injected into knee joint of normal rabbits respectively, every group was killed at different time postinjection, took out major organ and collected urine and blood, then weighted and determined their radio counts. HA particles, as a natural component of bone was known to have good compatibility with soft tissue and biodegrade into calcium and phosphate in vivo. It was readily prepared from common chemical and formed into particles of desired size range in a controlled process, it had high stability in vitro and vivo. Radiolabelling of HA particle with 166 Ho by EDTMP was simple to perform and provides an excellent labelling yield that was more than 95% under the optimal labelling condition. The optimal labelling condition at room temperature was pH 6.0-8.0 and vibration time 15 minutes. The absorbed capacity of HA particle was 5 mg Ho/g HA particle and size of radiolabelling particle was at range of 2-5,μm that is suitable for therapy of radiation synovectomy. 166 Ho-EDTMP-HA particle demonstrated high in vitro stability in either normal saline or 1% BSA solution, but instability under extremely acidic condition (pH 1-2). The control studies performed with 166 Ho-EDTMP not bound to HA particle provided information on the distribution of radioactivity that would occur upon leakage of the radiochemical compound from joint. Its short half-life, its extremely low leakage from the joint and its even distribution throughout
Wind profiler mixing depth and entrainment measurements with chemical applications

Energy Technology Data Exchange (ETDEWEB)

Angevine, W.M.; Trainer, M.; Parrish, D.D.; Buhr, M.P.; Fehsenfeld, F.C. [NOAA Aeronomy Lab., Boulder, CO (United States); Kok, G.L. [NCAR Research Aviation Facility, Boulder, CO (United States)

1994-12-31

Wind profiling radars operating at 915 MHz have been present at a number of regional air quality studies. The profilers can provide a continuous, accurate record of the depth of the convective mixed layer with good time resolution. Profilers also provide information about entrainment at the boundary layer top. Mixing depth data from several days of the Rural Oxidants in the Southern Environment II (ROSE II) study in Alabama in June, 1992 are presented. For several cases, chemical measurements from aircraft and ground-based instruments are shown to correspond to mixing depth and entrainment zone behavior observed by the profiler.
40Ar/39Ar Geochronology of Supergene K-bearing Sulfate Minerals: Cenozoic Continental Weathering, Landscape Evolution and Paleoclimates in the Tu-Ha Basin, Northwestern China

Science.gov (United States)

Yang, J.; Zheng, D.; Chen, W.; Hough, B.; Qiu, H.; Wang, W.; Wu, Y.; Yang, L.

2017-12-01

40Ar/39Ar incremental-heating analyses of supergene jarosite and yavapaiite from three weathering profiles at the Hongshan, Liuhuangshan, and Caihuagou deposits in the Tu-Ha Basin, China, were carried out to provide numerical constraints on the timing and duration of weathering and derive insights into local paleoclimatic and landscape evolution. Well-constrained plateau ages and best-fitting inverse isochrons have been obtained for 11 samples, yield 40Ar/39Ar ages ranging from 33.3±0.5 Ma to 3.3±0.4 Ma (1σ). Our 40Ar/39Ar ages, combined with the published ages, indicate that the high elevations sites hosting the most complete and complex weathering profiles present. The presence of ancient weathering ages in current outcrop in the Tu-Ha Basin suggests that denudation was not homogeneous, and the landscape evolution follow a scarp retreat model. These geochronological results suggest that a protracted history of weathering and supergene enrichment and, by inference, arid-semiarid climate (with at least a moderate amount of precipitation (>10 cm/y)) favorable to intense chemical weathering emerged at 33.3 Ma, 27.7-23.3 Ma, and 16.4-14.7 Ma, and prevailed from 11-7.8 Ma. Then, a progressive change from arid-semiarid towards hyperarid conditions and predominantly hyperarid conditions may have persisted since at least ca. 3.3 Ma. The climatic implications inferred from the weathering geochronology are in agreement with the chemical parameters and isotopic compositions of the Cenozoic sedimentary sequence from the Lianmuqin section in the Tu-Ha Basin, attesting to the reliability of weathering geochronology by the 40Ar/39Ar method as an indicator of paleoclimate in arid areas. Our results suggest that the retreat of the Paratethys Sea, which would have reduced eastward water vapor transport by the westerlies to the Tu-Ha Basin, led to its aridification in the Oligocene and that increased rain shadow effects, resulting from uplift of the Tibetan Plateau and Tian Shan

Hi ha un paper per a l'ètica a l'empresa?

OpenAIRE

Argandoña Rámiz, Antonio

2001-01-01

No hi ha decisions ètiques i decisions no ètiques. Hi ha decisions: l'ètica és, simplement, un component més de cada decisió i, per tant, molt important. No hi ha directors d'empresa ètics i altres que no ho són: hi ha directors que dirigeixen bé, i directors que dirigeixen malament. Els que dirigeixen bé són ètics. Si no són ètics, estan perdent algun aspecte de la realitat que és necessari per a la seva tasca de dirigir: per tant, dirigeixen malament. Però, a més a més, ser ètic no és tan ...
Iron(III) and manganese(II) substituted hydroxyapatite nanoparticles: Characterization and cytotoxicity analysis

International Nuclear Information System (INIS)

Li Yan; Nam, C T; Ooi, C P

2009-01-01

Calcium hydroxyapatite (HA) is the main inorganic component of natural bones and can bond to bone directly in vivo. Thus HA is widely used as coating material on bone implants due to its good osteoconductivity and osteoinductivity. Metal ions doped HA have been used as catalyst or absorbents since the ion exchange method has introduced new properties in HA which are inherent to the metal ions. For example, Mn 2+ ions have the potential to increase cell adhesion while Fe 3+ ions have magnetic properties. Here, Fe(III) substituted hydroxyapatite (Fe-HA) and Mn(II) substituted hydroxyapatite (Mn-HA) were produced by wet chemical method coupled with ion exchange mechanism. Compared with pure HA, the colour of both Fe-HA and Mn-HA nanoparticles changed from white to brown and pink respectively. The intensity of the colours increased with increasing substitution concentrations. XRD patterns showed that all samples were single phased HA while the FTIR spectra revealed all samples possessed the characteristic phosphate and hydroxyl adsorption bands of HA. However, undesired adsorption bands of carbonate substitution (B-type carbonated HA) and H 2 O were also detected, which was reasonable since the wet chemical method was used in the synthesis of these nanoparticles. FESEM images showed all samples were elongated spheroids with small size distribution and of around 70 nm, regardless of metal ion substitution concentrations. EDX spectra showed the presence of Fe and Mn and ICP-AES results revealed all metal ion substituted HA were non-stoichiometric (Ca/P atomic ratio deviates from 1.67). Fe-HA nanoparticles were paramagnetic and the magnetic susceptibility increased with the increase of Fe content. Based on the extraction assay for cytotoxicity test, both Fe-HA and Mn-HA displayed non-cytotoxicity to osteoblast.
Preparation and characterization of NiW-nHA composite catalyst for hydrocracking

Science.gov (United States)

Zhou, Gang; Hou, Yongzhao; Liu, Lei; Liu, Hongru; Liu, Can; Liu, Jing; Qiao, Huiting; Liu, Wenyong; Fan, Yubo; Shen, Shituan; Rong, Long

2012-11-01

The synthesis, characterization and catalytic capability of the NiW-nano-hydroxyapatite (NiW-nHA) composite were investigated in this paper. The NiW-nHA catalyst was prepared by a co-precipitation method. Then Fourier transform infrared (FT-IR) spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD) and energy dispersive spectroscopy (EDX) were used to analyze this material. In addition, the catalytic capacity of the NiW-nHA composite was also examined by FT-IR and gas chromatography (GC). The results of FT-IR analysis indicated that Ni, W and nHA combined closely. TEM observation revealed that this catalyst was needle shaped and the crystal retained a nanometer size. XRD data also suggested that a new phase of CaWO4 appeared and the lattice parameters of nHA changed in this system. nHA was the carrier of metals. The rates of Ni/W-loading were 73.24% and 65.99% according to the EDX data, respectively. Furthermore, the conversion of 91.88% Jatropha oil was achieved at 360 °C and 3 MPa h-1 over NiW-nHA catalyst. The straight chain alkanes ranging from C15 to C18 were the main components in the production. The yield of C15-C18 alkanes was up to 83.56 wt%. The reaction pathway involved hydrocracking of the C&z.dbd;C bonds of these triglycerides from Jatropha oil. This paper developed a novel non-sulfided catalyst to obtain a ``green biofuel'' from vegetable oil.
Gemini NIFS survey of feeding and feedback processes in nearby active galaxies - II. The sample and surface mass density profiles

Science.gov (United States)

Riffel, R. A.; Storchi-Bergmann, T.; Riffel, R.; Davies, R.; Bianchin, M.; Diniz, M. R.; Schönell, A. J.; Burtscher, L.; Crenshaw, M.; Fischer, T. C.; Dahmer-Hahn, L. G.; Dametto, N. Z.; Rosario, D.

2018-02-01

We present and characterize a sample of 20 nearby Seyfert galaxies selected for having BAT 14-195 keV luminosities LX ≥ 1041.5 erg s-1, redshift z ≤ 0.015, being accessible for observations with the Gemini Near-Infrared Field Spectrograph (NIFS) and showing extended [O III]λ5007 emission. Our goal is to study Active Galactic Nucleus (AGN) feeding and feedback processes from near-infrared integral-field spectra, which include both ionized (H II) and hot molecular (H2) emission. This sample is complemented by other nine Seyfert galaxies previously observed with NIFS. We show that the host galaxy properties (absolute magnitudes MB, MH, central stellar velocity dispersion and axial ratio) show a similar distribution to those of the 69 BAT AGN. For the 20 galaxies already observed, we present surface mass density (Σ) profiles for H II and H2 in their inner ˜500 pc, showing that H II emission presents a steeper radial gradient than H2. This can be attributed to the different excitation mechanisms: ionization by AGN radiation for H II and heating by X-rays for H2. The mean surface mass densities are in the range (0.2 ≤ ΣH II ≤ 35.9) M⊙ pc-2, and (0.2 ≤ ΣH2 ≤ 13.9)× 10-3 M⊙ pc-2, while the ratios between the H II and H2 masses range between ˜200 and 8000. The sample presented here will be used in future papers to map AGN gas excitation and kinematics, providing a census of the mass inflow and outflow rates and power as well as their relation with the AGN luminosity.
Investigation of microstructure and mechanical properties of PLLA/HA system

Science.gov (United States)

Nik Hanyn N., H.; Ahmad, K. Z. Ku; Yusof, Mohd Reusmaazran; Kamarolzaman, A. A.

2017-12-01

The poly(L-lactide) (PLLA), carboxymethyl starch (CMS) and hydroxyapatite (HAp) nanofibers have been prepared by electrospinning technique. The PLLA/CMS formulations applied at various voltage were studied for electrospinning into nanofibers. It was found that the morphology of the fibers became finer with the increment of voltage applied. The formulation with 10 kV voltage was selected as it shows promising result based on the morphology observation. The PLLA/CMS/HA formulation with HA loading concentration (i.e. 0.25, 0.50, 0.75 and 1.00 wt%) was fabricated in the next stage, and the effect of HA addition was studied. Observation showed that the electrospun nanofibers' diameters were reduced with the addition of HAp concentration from 0.25 wt% HAp to 1.00 wt% Hap.
Mao & Püha Eliisabet / Jüri Kuuskemaa

Index Scriptorium Estoniae

Kuuskemaa, Jüri, 1942-

2000-01-01

Kadrioru lossis Väliskunstimuuseumi ekspositsioonis olevast antikvaar August Käbi pärandist pärit maalist. Kunstiteadlase Mai Levini arvates võib maali autoriks olla Rooma kunstnik Tommaso Salini, hüüdnimega Mao. Maali süzee: "Püha Eliisabeti kuulutus". 1 ill
Gene expression profiles in stages II and III colon cancers

DEFF Research Database (Denmark)

Thorsteinsson, Morten; Kirkeby, Lene T; Hansen, Raino

2012-01-01

PURPOSE: A 128-gene signature has been proposed to predict outcome in patients with stages II and III colorectal cancers. In the present study, we aimed to reproduce and validate the 128-gene signature in external and independent material. METHODS: Gene expression data from the original material...... were retrieved from the Gene Expression Omnibus (GEO) (n¿=¿111) in addition to a Danish data set (n¿=¿37). All patients had stages II and III colon cancers. A Prediction Analysis of Microarray classifier, based on the 128-gene signature and the original training set of stage I (n¿=¿65) and stage IV (n...... correctly predicted as stage IV-like, and the remaining patients were predicted as stage I-like and unclassifiable, respectively. Stage II patients could not be stratified. CONCLUSIONS: The 128-gene signature showed reproducibility in stage III colon cancer, but could not predict recurrence in stage II...
Stratospheric ozone profile and total ozone trends derived from the SAGE I and SAGE II data

Science.gov (United States)

Mccormick, M. P.; Veiga, Robert E.; Chu, William P.

1992-01-01

Global trends in both stratospheric column ozone and as a function of altitude are derived on the basis of SAGE I/II ozone data from the period 1979-1991. A statistical model containing quasi-biennial, seasonal, and semiannual oscillations, a linear component, and a first-order autoregressive noise process was fit to the time series of SAGE I/II monthly zonal mean data. The linear trend in column ozone above 17-km altitude, averaged between 65 deg S and 65 deg N, is -0.30 +/-0.19 percent/yr, or -3.6 percent over the time period February 1979 through April 1991. The data show that the column trend above 17 km is nearly zero in the tropics and increases towards the high latitudes with values of -0.6 percent/yr at 60 deg S and -0.35 percent/yr at 60 deg N. Both these results are in agreement with the recent TOMS results. The profile trend analyses show that the column ozone losses are occurring below 25 km, with most of the loss coming from the region between 17 and 20 km. Negative trend values on the order of -2 percent/yr are found at 17 km in midlatitudes.
Prediction of IOI-HA Scores Using Speech Reception Thresholds and Speech Discrimination Scores in Quiet

DEFF Research Database (Denmark)

Brännström, K Jonas; Lantz, Johannes; Nielsen, Lars Holme

2014-01-01

), and speech discrimination scores (SDSs) in quiet or in noise are common assessments made prior to hearing aid (HA) fittings. It is not known whether SRT and SDS in quiet relate to HA outcome measured with the International Outcome Inventory for Hearing Aids (IOI-HA). PURPOSE: The aim of the present study...... COLLECTION AND ANALYSIS: The psychometric properties were evaluated and compared to previous studies using the IOI-HA. The associations and differences between the outcome scores and a number of descriptive variables (age, gender, fitted monaurally/binaurally with HA, first-time/experienced HA users, years...
El griot no ha muerto, viva el hip-hop

Directory of Open Access Journals (Sweden)

Fco. Javier González García-Mamely

2015-11-01

Full Text Available La oratura en África ha jugado un papel vital a lo largo de los milenios como un (único medio de preservar y transmitir la historia, la cultura y el imaginario de cada comunidad. El griot es un claro modelo de las artes orales, pero ha sufrido cambios drásticos en su modo de vida y en los recursos narrativos de los que dispone. Tras el mecenazgo de los grandes reyes y las sucesivas llegadas del islam, el colonialismo y los procesos de modernización y occidentalización, los griots se han tenido que adaptar a nuevos patrones, audiencias y medios de comunicación, convirtiéndose en políticos o en figuras del espectáculo. La aparición del rap y el hip-hop reclamando ser los “modernos griots” ha ocasionado una respuesta crítica y una atracción viral hacia el nuevo fenómeno, sea éste un nuevo género o una reinvención de la oratura del antiguo griot.
Changes of serum HA and LN level in patients with pulmonary tuberculosis after therapy

International Nuclear Information System (INIS)

Yu Jing Zhang Hongwei; Li Jie

2007-01-01

Objective: To study the serum HA and LN level in patients with pulmonary tuberculosis and their response to therapy. Methods: Serum HA and LN levels were measured with RIA in: (1) 42 patients with active pulmonary tuberculosis both before and after 2 months' therapy, (2) 40 patients with non-active pulmonary TB and 330 controls. Results: The serum HA and LN level in patients with active pulmonary tubemulosis were significantly higher than those in controls (P < 0.01 ), while no significant difference could Be found Between the levels in patients with non-active pulmonary tuberculosis and controls. The serum HA and LN level in patients with active pulmonary tuberculosis dropped after therapy, but were still higher than those in controls. Significant correlation could Be found between HA and LN levels (P<0.01). The HA and LN levels were positively correlated with severity of the disease, ESR and CRP contents. Conclusion: The measurement of serum HA and LN is valuable for early diagnosis, monitoring development and assessment of therapeutic effect in patients with pulmonary tubemulosis. (authors)
Biomarker Profiles in Women with PCOS and PCOS Offspring; A Pilot Study

NARCIS (Netherlands)

Daan, Nadine M P; Koster, Maria P H; de Wilde, Marlieke A; Dalmeijer, Gerdien W; Evelein, Annemieke M V; Fauser, Bart C J M; de Jager, Wilco

2016-01-01

OBJECTIVE: To study metabolic/inflammatory biomarker risk profiles in women with PCOS and PCOS offspring. DESIGN: Cross-sectional comparison of serum biomarkers. SETTING: University Medical Center Utrecht. PATIENTS: Hyperandrogenic PCOS women (HA-PCOS, n = 34), normoandrogenic PCOS women (NA-PCOS, n
166Ho-HA evaluation as therapeutic agent for rheumatoid arthritis treatment

International Nuclear Information System (INIS)

Chandia, M.C.; Errazu, X.C.; Pinto, L.N.; Godoy, N.O.; Avila, M.J.; Mendoza, P.; Mendoza, J.; Jofre, J.; Sirraalta, P.

2002-01-01

Aim: Rheumatoid arthritis is a limiting disease having, among its pathological features, the inflammation of synovial tissue with progressive and later destruction of the articulation. This leads to joint deformation and loss of its function, generating pain and reducing the mobility of the affected articulation. The aim was to evaluate 166 Ho-Hydroxyapatite ( 166 Ho-HA) as potential radiopharmaceutical for the symptomatic treatment of chronic and acute arthritis. Materials and Methods: Holmiun-166 was produced by irradiation of Ho 2 O 3 at La Reina Research Reactor, Nuclear Chilean Energy Commission. Hydroxyapatite was in-house synthesized. Its labelling and quality controls follows the internationally accepted procedures. An antigen's arthritis was induced to eight New Zealand rabbits with the 166 Ho-HA radiochemical being administered thereafter in two dosage modalities (single and double). The compound therapeutic efficiency was evaluated based upon clinical improvement and images from the inflamated articulation using 67 Ga citrate before and after 166 Ho-HA injection. Results: The radiochemical purity of the inoculated compound was greater than 98% as measured under sterility conditions. Clinically, an inflammation reduction (2 cm), appetite improvement and general well being was observed. The 166 Ho-HA distribution and localization was monitored using gamma camera images taken at 4 and 24 h. There was no evidence of extra articular leakage. From the 67 Ga citrate imaging, the acute group shows an overall improvement of well being corresponding to a lesser uptake at the inflamated articulation, regarding to the chronic group. The 166 Ho-HA double doses, compared to the single doses, suggest a reduced uptake of 67 Ga citrate at the inflamated tissue, meaning an increased therapeutic effect. Conclusions: 166 Ho-HA is useful as therapeutic agent for the symptomatic treatment of rheumatoid arthritis as shown by imaging and clinical examination
166 Ho-HA Evaluation as therapeutic agent for rheumatoid arthritis treatment

International Nuclear Information System (INIS)

Chandia, M; Errazu, X; Mendoza, P; Troncoso, F; Jofre, J; Sierralta, P

2003-01-01

Aim: Rheumatoid arthritis is a limiting disease having, among its pathological features, the inflammation of synovial tissue with progressive and later destruction of the articulation. This lead to joint deformation and loss of its function, generating pain and reducing the mobility of the affected articulation. The aim was to evaluate 166 Ho-Hydroxyapatite ( 166 Ho-HA) as potential radiopharmaceutical for the syntomatic treatment of chronic and acute arthritis Materials and Methods: 166 Holmiun was produced by irradiation of Ho 2 O 3 at La Reina Research Reactor, Nuclear Chilean Energy Commission. Hydroxyapatite was in-house synthetized. Its labelling and quality controls follows the internationally accepted procedures. An antigen arthritis was induced to eight New Zealand rabbits with the 166 Ho-HA radiochemical being administred thereafter in two dosage modalities (single and double). The compound therapeutic efficiency was evaluated based upon clinical improvement and images from the inflamated articulation using 67 Ga citrate before and after 166 Ho-HA injection. Results: The radiochemical purity of the innoculated compound was greater than 98% as measured under sterility conditions. Clinically, an inflamation reduction (2 cm), appetite improvement and general well being was observed. The 166 Ho-HA distribution and localization was monitored using gamma camera images taken at 4 and 24 h. There was no evidence of extraarticular leakage. From the 67 Ga citrate imaging, the acute group shows an overall improvement of well being corresponding to a lesser uptake at the inflamated articulation, regarding to the chronic group. The 166 Ho-HA double dosis, compared to the single dosis, suggest a reduced uptake of 67 Ga citrate at the inflamated tissue, meaning an increased therapeutic effect. Conclusions: 166 Ho-HA is usefull as therapeutic agent for the syntomatic treatment of rheumatoideal arthritis as shown by imaging and clinical examination (author)
Mechanical, thermal, rheological and morphological behaviour of irradiated PP/HA composites

International Nuclear Information System (INIS)

Ramirez, C.; Albano, C.; Karam, A.; Dominguez, N.; Sanchez, Y.; Gonzalez, G.

2005-01-01

Hydroxyapatite (HA) reinforced polypropylene (PP) composites are being developed as bone graft materials. In this research, the effect of γ irradiation on mechanical, rheological, thermal and morphological behaviour of PP-HA composites was studied. The melt flow index of polymer increased markedly when it was exposed to radiation. This is indicative of chain scission reaction as the predominant process. During the tensile testing, the composites exhibited brittle behaviour, showing no fluency point. Elongation at break showed a tendency to decrease with the increase in radiation dose while stress at break did not show significant variation with radiation dose. High HA content (>20%) and radiation dose (25 kGy) had significant influence on thermal stability
Modulating protein release profiles by incorporating hyaluronic acid into PLGA microparticles Via a spray dryer equipped with a 3-fluid nozzle

DEFF Research Database (Denmark)

Wan, Feng; Maltesen, Morten Jonas; Andersen, Sune Klint

2014-01-01

with or without HA were prepared using a spray dryer equipped with a 3-fluid nozzle. The effects of HA on the surface tension and the rheological behavior of the inner feed solution were investigated. The physicochemical properties of the resulting microparticles were characterized using scanning electron......: The present work demonstrates the potential of HA to modulate protein release profile from PLGA microparticle formulations produced via spray drying using 3-fluid nozzle....
Non-LTE profiles of strong solar lines

Science.gov (United States)

Schneeberger, T. J.; Beebe, H. A.

1976-01-01

The complete linearization method is applied to the formation of strong lines in the solar atmosphere. Transitions in Na(I), Mg(I), Ca(I), Mg(II), and Ca(II) are computed with a standard atmosphere and microturbulent velocity model. The computed profiles are compared to observations at disk center.
Methane oxidation in soil profiles of Dutch and Finnish coniferous forests with different soil texture and atmospheric nitrogen deposition

NARCIS (Netherlands)

Saari, A.; Martikainen, P.J.; Ferm, A.; Ruuskanen, J.; De Boer, W.; Troelstra, S.R.; Laanbroek, H.J.

1997-01-01

We studied methane oxidation capacity in soil profiles of Dutch and Finnish coniferous forests. The Finnish sites (n = 9) had nitrogen depositions from 3 to 36 kg N ha(-1) a(-1). The deposition of N on the Dutch sites (n = 13) was higher ranging from 50 to 92 kg N ha(-1) a(-1). The Dutch sites had
Enhancement of mucosal immune responses by chimeric influenza HA/SHIV virus-like particles

International Nuclear Information System (INIS)

Guo Lizheng; Lu Xiaoyan; Kang, S.-M.; Chen Changyi; Compans, Richard W.; Yao Qizhi

2003-01-01

To enhance mucosal immune responses using simian/human immunodeficiency virus-like particles (SHIV VLPs), we have produced novel phenotypically mixed chimeric influenza HA/SHIV VLPs and used them to immunize C57BL/6J mice intranasally. Antibody and cytotoxic T-cell (CTL) responses as well as cytokine production in both systemic and mucosal sites were compared after immunization with SHIV VLPs or chimeric HA/SHIV VLPs. By using enzyme-linked immunosorbent assay (ELISA), the levels of serum IgG and mucosal IgA to the HIV envelope protein (Env) were found to be highest in the group immunized with chimeric HA/SHIV VLPs. Furthermore, the highest titer of serum neutralizing antibody against HIV Env was found with the group immunized with chimeric HA/SHIV VLPs. Analysis of the IgG1/IgG2a ratio indicated that a T H 1-oriented immune response resulted from these VLP immunizations. HA/SHIV VLP-immunized mice also showed significantly higher CTL responses than those observed in SHIV VLP-immunized mice. Moreover, a MHC class I restricted T-cell activation ELISPOT assay showed a mixed type of T H 1/T H 2 cytokines in the HA/SHIV VLP-immunized mice, indicating that the chimeric VLPs can enhance both humoral and cellular immune responses to the HIV Env protein at multiple mucosal and systemic sites. The results indicate that incorporation of influenza HA into heterotypic VLPs may be highly effective for targeting vaccines to mucosal surfaces
3D bioprinting of methacrylated hyaluronic acid (MeHA) hydrogel with intrinsic osteogenicity.

Science.gov (United States)

Poldervaart, Michelle T; Goversen, Birgit; de Ruijter, Mylene; Abbadessa, Anna; Melchels, Ferry P W; Öner, F Cumhur; Dhert, Wouter J A; Vermonden, Tina; Alblas, Jacqueline

2017-01-01

In bone regenerative medicine there is a need for suitable bone substitutes. Hydrogels have excellent biocompatible and biodegradable characteristics, but their visco-elastic properties limit their applicability, especially with respect to 3D bioprinting. In this study, we modified the naturally occurring extracellular matrix glycosaminoglycan hyaluronic acid (HA), in order to yield photo-crosslinkable hydrogels with increased mechanical stiffness and long-term stability, and with minimal decrease in cytocompatibility. Application of these tailor-made methacrylated hyaluronic acid (MeHA) gels for bone tissue engineering and 3D bioprinting was the subject of investigation. Visco-elastic properties of MeHA gels, measured by rheology and dynamic mechanical analysis, showed that irradiation of the hydrogels with UV light led to increased storage moduli and elastic moduli, indicating increasing gel rigidity. Subsequently, human bone marrow derived mesenchymal stromal cells (MSCs) were incorporated into MeHA hydrogels, and cell viability remained 64.4% after 21 days of culture. Osteogenic differentiation of MSCs occurred spontaneously in hydrogels with high concentrations of MeHA polymer, in absence of additional osteogenic stimuli. Addition of bone morphogenetic protein-2 (BMP-2) to the culture medium further increased osteogenic differentiation, as evidenced by increased matrix mineralisation. MeHA hydrogels demonstrated to be suitable for 3D bioprinting, and were printed into porous and anatomically shaped scaffolds. Taken together, photosensitive MeHA-based hydrogels fulfilled our criteria for cellular bioprinted bone constructs within a narrow window of concentration.

3D bioprinting of methacrylated hyaluronic acid (MeHA hydrogel with intrinsic osteogenicity.

Directory of Open Access Journals (Sweden)

Michelle T Poldervaart

Full Text Available In bone regenerative medicine there is a need for suitable bone substitutes. Hydrogels have excellent biocompatible and biodegradable characteristics, but their visco-elastic properties limit their applicability, especially with respect to 3D bioprinting. In this study, we modified the naturally occurring extracellular matrix glycosaminoglycan hyaluronic acid (HA, in order to yield photo-crosslinkable hydrogels with increased mechanical stiffness and long-term stability, and with minimal decrease in cytocompatibility. Application of these tailor-made methacrylated hyaluronic acid (MeHA gels for bone tissue engineering and 3D bioprinting was the subject of investigation. Visco-elastic properties of MeHA gels, measured by rheology and dynamic mechanical analysis, showed that irradiation of the hydrogels with UV light led to increased storage moduli and elastic moduli, indicating increasing gel rigidity. Subsequently, human bone marrow derived mesenchymal stromal cells (MSCs were incorporated into MeHA hydrogels, and cell viability remained 64.4% after 21 days of culture. Osteogenic differentiation of MSCs occurred spontaneously in hydrogels with high concentrations of MeHA polymer, in absence of additional osteogenic stimuli. Addition of bone morphogenetic protein-2 (BMP-2 to the culture medium further increased osteogenic differentiation, as evidenced by increased matrix mineralisation. MeHA hydrogels demonstrated to be suitable for 3D bioprinting, and were printed into porous and anatomically shaped scaffolds. Taken together, photosensitive MeHA-based hydrogels fulfilled our criteria for cellular bioprinted bone constructs within a narrow window of concentration.
A Modified ELISA Accurately Measures Secretion of High Molecular Weight Hyaluronan (HA) by Graves' Disease Orbital Cells

Science.gov (United States)

Krieger, Christine C.

2014-01-01

Excess production of hyaluronan (hyaluronic acid [HA]) in the retro-orbital space is a major component of Graves' ophthalmopathy, and regulation of HA production by orbital cells is a major research area. In most previous studies, HA was measured by ELISAs that used HA-binding proteins for detection and rooster comb HA as standards. We show that the binding efficiency of HA-binding protein in the ELISA is a function of HA polymer size. Using gel electrophoresis, we show that HA secreted from orbital cells is primarily comprised of polymers more than 500 000. We modified a commercially available ELISA by using 1 million molecular weight HA as standard to accurately measure HA of this size. We demonstrated that IL-1β-stimulated HA secretion is at least 2-fold greater than previously reported, and activation of the TSH receptor by an activating antibody M22 from a patient with Graves' disease led to more than 3-fold increase in HA production in both fibroblasts/preadipocytes and adipocytes. These effects were not consistently detected with the commercial ELISA using rooster comb HA as standard and suggest that fibroblasts/preadipocytes may play a more prominent role in HA remodeling in Graves' ophthalmopathy than previously appreciated. PMID:24302624
Inhibitory effect of PTD-OD-HA fusion protein on Bcr-Abl in K562 cells

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Miao GAO

2012-10-01

Full Text Available Objective　To study the transduction dynamics, location of PTD-OD-HA fusion protein and its interaction with Bcr-Abl oncoprotein in K562 cell lines, and explore the influence of PTD-OD-HA fusion protein on oligomerization and tyrosine kinase activity of Bcr-Abl. Methods　PTD-OD-HA fusion protein was labeled with FITC and co-cultured with K562 cells. The transduction efficiency of labeled PTD-OD-HA at different doses and time intervals was observed under fluorescence microscope. The location of labeled PTD-OD-HA fusion protein in K562 cells was detected by confocal microscopy. The interaction of PTD-OD-HA fusion protein with Bcr-Abl oncoprotein was confirmed by coimmunoprecipitation. The phosphorylation of Bcr-Abl oncoprotein was detected by Western blotting. Results　PTD-OD-HA fusion protein labeled with FITC was transduced into K562 cells in a dose- and time-dependent manner. PTD-OD-HA fusion protein was located in the cytoplasm of K562 cells and was consistent with the location of Bcr-Abl oncoprotein. The interaction of PTD-OD-HA fusion protein with Bcr-Abl oncoprotein was proved in K562 cells. This interaction could interrupt the homologous oligomerization of Bcr-Abl oncoprotein and reduce the phosphorylation of Bcr-Abl oncoprotein. Conclusion　PTD-OD-HA fusion protein could be transduced into K562 cells efficiently, inhibit the oligomerization and reduce the phosphorylation of Bcr-Abl oncoprotein.
Ahmet Haşim’de Ev-Yuva ve Doğa İmgesi Home-Nest and Nature Images in Ahmet Haşim

Directory of Open Access Journals (Sweden)

Refika ALTIKULAÇ DEMİRDAĞ

2012-12-01

Full Text Available Home and Nest images are commonly used images in poetry. Home is a shelter within the poet feels himself safe and secure. Image of nature in Ahmet Hasim’s poems, has been the most focused subjects on. Furthermore, assessments have been made on the poet's pessimism and desire that escape to an imaginary world from the real world. But, It hasn’t been dwelled enough on subject why Haşim escape from the real world to an imaginary world. Because, imaginary world that was created in Haşim’s poems, is richer than the real world. It is alsa a remarkable issue, the need of Haşim for a home and a nest or a shelter in nature. Haşim is more peaceful in a narrower space, inside the home. We can see this situation in poet’s poem called "Evim". Furthermore Hashim described a larger shelter in his poem called "O Belde”. “O Belde” is a part of nature and a nook in nature. Therefore, just like abird's nest, it has been made with the poet's feelings, his heart. Actuallyit is not important to be real or not for poet. Because in fact the poetknows the place that described, is mind’s shelter. It is the poet’s mindand dream, who wants to escape from the real world. His mind wants toescape from the real world, Because the real world is a place that ispessimistic and unsafe. There is plenty of "movement" and plenty oflight. This plenty of movement and light has scared the poet. He canfind the world that is less bright, more silent, more and more intimatewith nature, in his "untouched" regions of his mind. This is the placethat he found is home-nest, “O Belde”. Ev ve yuva imgeleri şiirde çok kullanılan imgelerdendir. Şairin kendini güvende hissettiği bir sığınaktır ev. Ahmet Haşim’in şiirlerinde doğa imgesi üzerinde en fazla durulan konulardan olmuştur. Ayrıca şairin karamsarlığı ve gerçek dünyadan hayali dünyaya kaçma isteği üzerinde de değerlendirmeler yapılmıştır. Fakat Haşim’in neden gerçek d
Experimental study on bone tissue reaction around HA implants radiated after implantation

International Nuclear Information System (INIS)

Kudo, Masato; Matsui, Yoshiro; Tamura, Sayaka; Chen, Xuan; Uchida, Haruo; Mori, Kimie; Ohno, Kohsuke; Michi, Ken-ichi

1998-01-01

This study was conducted to investigate histologically and histomorphometrically the tissue reaction around hydroxylapatite (HA) implants that underwent irradiation in 3 different periods in the course of bone healing after implantation. The cylindrical high-density HA implants were implanted in 48 Japanese white rabbit mandibles. A single 15 Gy dose was applied to the mandible 5, 14, or 28 days after implantation. The rabbits were sacrificed 7, 14, 28, and 90 days after irradiation. Nonirradiated rabbits were used as controls. CMR, labeling with tetracycline and calcein, and non-decalcified specimens stained with toluidine blue were used for histological analyses and histomorphometric measurements. The results were as follows: In the rabbits irradiated 5 days after implantation, the HA-bone contact was observed later than that in the controls and the bone-implant contact surface ratio was lower than that in the controls at examination because necrosis of the newly-formed bone occurred just after irradiation. HA-bone contact of the rabbits irradiated 14 and 28 days after implantation was similar to that of the controls. And, bone remodeling was suppressed in rabbits of each group sacrificed at 90 days after irradiation. The results suggested that a short interval between implantation and irradiation causes direct contact between HA implant and bone and a long lapse of time before irradiation hardly affects the bone-implant contact, but delays bone remodeling. Therefore, it is necessary to prevent overloading the HA implants irradiated after implantation and pay utmost attention to conditions around the bone-implant contact. (author)
New therapeutic agent for radiation synovectomy - preparation of {sup 166}Ho-EDTMP-HA particle

Energy Technology Data Exchange (ETDEWEB)

Bai, H.; Jin, X.; Du, J.; Wang, F.; Chen, D.; Fan, H.; Cheng, Z.; Zhang, J. [China Institute of Atomic Energy, Beijing (Switzerland). Isotope Department

1997-10-01

In order to prepare new therapeutical agent for radiation synovectomy, Hydroxyapatite (HA) was labelled with {sup 166}Ho by EDTMP that had high affinity to HA particles. Radiolabelling of HA particles was divided into two steps, {sup 166}Ho-EDTMP was prepared first; then mixed with HA particles completely and vibrated for 15 minutes on the micromixer at room temperature, washed 3 times with deionized water. Radiolabelling particle was separated from free {sup 166}Ho via centrifugation to determine its radiolabelling efficiency. {sup 166}Ho-EDTMP-HA and {sup 166}Ho-EDTMP were injected into knee joint of normal rabbits respectively, every group was killed at different time postinjection, took out major organ and collected urine and blood, then weighted and determined their radio counts. HA particles, as a natural component of bone was known to have good compatibility with soft tissue and biodegrade into calcium and phosphate in vivo. It was readily prepared from common chemical and formed into particles of desired size range in a controlled process, it had high stability in vitro and vivo. Radiolabelling of HA particle with {sup 166}Ho by EDTMP was simple to perform and provides an excellent labelling yield that was more than 95% under the optimal labelling condition. The optimal labelling condition at room temperature was pH 6.0-8.0 and vibration time 15 minutes. The absorbed capacity of HA particle was 5 mg Ho/g HA particle and size of radiolabelling particle was at range of 2-5,{mu}m that is suitable for therapy of radiation synovectomy. {sup 166}Ho-EDTMP-HA particle demonstrated high in vitro stability in either normal saline or 1% BSA solution, but instability under extremely acidic condition (pH 1-2). The control studies performed with {sup 166}Ho-EDTMP not bound to HA particle provided information on the distribution of radioactivity that would occur upon leakage of the radiochemical compound from joint. Its short half-life, its extremely low leakage from the
Sintering behavior and property of bioglass modified HA-Al2O3 composite

Directory of Open Access Journals (Sweden)

Wang Li-li

2012-01-01

Full Text Available The bioglass modified HA-Al2O3 composites were successfully fabricated by mixing HA, synthesized by wet chemical method between precursor materials H3PO4 and Ca(OH2, with 25wt% Al2O3 and different content of bioglass (5%, 25%, 45%, 65wt% respectively, with a mole fraction of 53.9%SiO2, 22.6%Na2O, 21.8%CaO, and 1.7wt%P2O5, sintered in air at various temperatures (750-950°C for 2h. when the content of bioglass is below 45wt% in the composite, HA decomposes completely and transforms to β-TCP. The main phase in this case are β-TCP, Al2O3 and Ca3(AlO32.When the content of bioglass is above 45wt% in the composite, the decomposition of HA to β-TCP is suppressed and the main phases in this case are Al2O3 and HA, DCP□CaHPO4□ and β-TCP, which almost have the same chemical composition, forming ternary-glass phase, and have better bioactive than pure HA. It can also be found that at the certain addition of bioglass, the higher sintered temperature, the bigger volume density and flexural strength of the composite are, but when the sintered temperature reaches 950°C, they decrease. This modified HA-Al2O3 composites by calcium silicate glass have a much lower sintering temperature and decrease the production cost much.
HaVec: An Efficient de Bruijn Graph Construction Algorithm for Genome Assembly

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Md Mahfuzer Rahman

2017-01-01

Full Text Available Background. The rapid advancement of sequencing technologies has made it possible to regularly produce millions of high-quality reads from the DNA samples in the sequencing laboratories. To this end, the de Bruijn graph is a popular data structure in the genome assembly literature for efficient representation and processing of data. Due to the number of nodes in a de Bruijn graph, the main barrier here is the memory and runtime. Therefore, this area has received significant attention in contemporary literature. Results. In this paper, we present an approach called HaVec that attempts to achieve a balance between the memory consumption and the running time. HaVec uses a hash table along with an auxiliary vector data structure to store the de Bruijn graph thereby improving the total memory usage and the running time. A critical and noteworthy feature of HaVec is that it exhibits no false positive error. Conclusions. In general, the graph construction procedure takes the major share of the time involved in an assembly process. HaVec can be seen as a significant advancement in this aspect. We anticipate that HaVec will be extremely useful in the de Bruijn graph-based genome assembly.
Elastin-like protein-hyaluronic acid (ELP-HA) hydrogels with decoupled mechanical and biochemical cues for cartilage regeneration.

Science.gov (United States)

Zhu, Danqing; Wang, Huiyuan; Trinh, Pavin; Heilshorn, Sarah C; Yang, Fan

2017-05-01

Hyaluronic acid (HA) is a major component of cartilage extracellular matrix and is an attractive material for use as 3D injectable matrices for cartilage regeneration. While previous studies have shown the promise of HA-based hydrogels to support cell-based cartilage formation, varying HA concentration generally led to simultaneous changes in both biochemical cues and stiffness. How cells respond to the change of biochemical content of HA remains largely unknown. Here we report an adaptable elastin-like protein-hyaluronic acid (ELP-HA) hydrogel platform using dynamic covalent chemistry, which allows variation of HA concentration without affecting matrix stiffness. ELP-HA hydrogels were created through dynamic hydrazone bonds via the reaction between hydrazine-modified ELP (ELP-HYD) and aldehyde-modified HA (HA-ALD). By tuning the stoichiometric ratio of aldehyde groups to hydrazine groups while maintaining ELP-HYD concentration constant, hydrogels with variable HA concentration (1.5%, 3%, or 5%) (w/v) were fabricated with comparable stiffness. To evaluate the effects of HA concentration on cell-based cartilage regeneration, chondrocytes were encapsulated within ELP-HA hydrogels with varying HA concentration. Increasing HA concentration led to a dose-dependent increase in cartilage-marker gene expression and enhanced sGAG deposition while minimizing undesirable fibrocartilage phenotype. The use of adaptable protein hydrogels formed via dynamic covalent chemistry may be broadly applicable as 3D scaffolds with decoupled niche properties to guide other desirable cell fates and tissue repair. Copyright © 2017 Elsevier Ltd. All rights reserved.
A Study of Hybrid Composite Hydroxyapatite (HA-Geopolymers as a Material for Biomedical Application

Directory of Open Access Journals (Sweden)

Saleha

2017-01-01

Full Text Available The main purpose of this research is to study the physical properties and microstructure characters of hybrid composites HA-geopolymers as a material for biomedical application. Hybrid composite HA–geopolymers were produced through alkaline activation method of metakaolin as a matrix and HA as the filler. HA was synthesized from eggshell particles by using a precipitation method. The addition of HA in metakaolin paste was varied from 0.5%, 1.0%, 1.5%, and 2.0% relative the weight of metakaolin. FTIR was used to examine the absorption bands the composites. X-ray diffraction (XRD was used to study the crystal structure of the starting and the resulting materials. Scanning Electron Microscopy-Energy Dispersive Spectroscopy (SEM-EDS was used to investigate the surface morphology of the composites. The thermal properties of the samples was examined by means of Differential Scanning Calorimetry (DSC. Capacitance measurement was conducted to investigate the bioactive properties of HA. The study results suggest that hybrid composite HA-geopolymers has a potential to be applied as a biomedical such as biosensor material.
{sup 166} Ho-HA Evaluation as therapeutic agent for rheumatoid arthritis treatment; Evaluacion de {sup 166}Ho-Ha como agente terapeutico en el tratamiento de la artritis reumatoidea

Energy Technology Data Exchange (ETDEWEB)

Chandia, M; Errazu, X [Comision Chilena de Energia Nuclear, Santiago (Chile); Mendoza, P [Departamento de Medicina Nuclear, Hospital Militar, Santiago (Chile); Troncoso, F [Comision Chilena de Energia Nuclear, Santiago (Chile); Jofre, J; Sierralta, P [Departamento de Medicina Nuclear, Hospital Militar, Santiago (Chile)

2003-01-01

Aim: Rheumatoid arthritis is a limiting disease having, among its pathological features, the inflammation of synovial tissue with progressive and later destruction of the articulation. This lead to joint deformation and loss of its function, generating pain and reducing the mobility of the affected articulation. The aim was to evaluate {sup 166}Ho-Hydroxyapatite ({sup 166} Ho-HA) as potential radiopharmaceutical for the syntomatic treatment of chronic and acute arthritis Materials and Methods: {sup 166}Holmiun was produced by irradiation of Ho{sub 2}O{sub 3} at La Reina Research Reactor, Nuclear Chilean Energy Commission. Hydroxyapatite was in-house synthetized. Its labelling and quality controls follows the internationally accepted procedures. An antigen arthritis was induced to eight New Zealand rabbits with the {sup 166}Ho-HA radiochemical being administred thereafter in two dosage modalities (single and double). The compound therapeutic efficiency was evaluated based upon clinical improvement and images from the inflamated articulation using {sup 67}Ga citrate before and after {sup 166} Ho-HA injection. Results: The radiochemical purity of the innoculated compound was greater than 98% as measured under sterility conditions. Clinically, an inflamation reduction (2 cm), appetite improvement and general well being was observed. The {sup 166} Ho-HA distribution and localization was monitored using gamma camera images taken at 4 and 24 h. There was no evidence of extraarticular leakage. From the {sup 67}Ga citrate imaging, the acute group shows an overall improvement of well being corresponding to a lesser uptake at the inflamated articulation, regarding to the chronic group. The {sup 166}Ho-HA double dosis, compared to the single dosis, suggest a reduced uptake of {sup 67}Ga citrate at the inflamated tissue, meaning an increased therapeutic effect. Conclusions: {sup 166} Ho-HA is usefull as therapeutic agent for the syntomatic treatment of rheumatoideal arthritis
Hamster endogenous retrovirus (HaER) - distinct properties of structural proteins and DNA polymerase

International Nuclear Information System (INIS)

Goldschmied-Reouven, A.; Yaniv, A.

1983-01-01

The structural proteins as well as some features of the RNA-dependent DNA polymerase of the hamster endogenous retrovirus (HaER) were examined. The polypeptide pattern of this virus is substantially different from that of other known retroviruses in containing major polypeptides with molecular weights of 68000, 59000, 27000, 24000 daltons. Double antibody competitive radioimmunoassays showed that the HaER particles do not share any detectable antigenic relatedness with the murine viruses' p30, but manifest a considerable relatedness with the feline leukemia virus p27 and a slight cross-reactivity with the rat virus major protein. The RNA-dependent DNA polymerase of HaER virus has a molecular size of approximately 73000 daltons and in contrast to other mammalian retroviruses shows no significant preference for Mn 2+ over Mg 2+ . Apart from the lack of antigenic relatedness between the HaER virus proteins and the p30 protein of murine viruses, there is also no antigenic relatedness between HaER and murine viruses insofar as their DNA polymerase is concerned. (Author)
Läkaköha - aktuaalne uurimisteema / Marje Oona

Index Scriptorium Estoniae

Oona, Marje, 1963-

2012-01-01

TÜ peremeditsiini õppetooli töötajate poolt algatatud uurimistööst, mille eesmärgiks on uurida Bordetella spp. infektsioonide epidemioloogiat, molekulaargeneetikat ja kliinilisi eripärasid ning selgitada läkaköha sagedasema diagnsimise põhjusi Eestis
The Technology and Properties of Digital Double Pulse Electrodepositing Ni-HA Composite Coating of Bioceramics

Institute of Scientific and Technical Information of China (English)

DONG He-yan; WANG Zhou; SHI Gu-guizhi; FU Chuan-qi; CHEN Wei-rong; JIN Zhong-hong; LI Yan

2004-01-01

This article discusses and analyses the technology, the surface image, microstructure and ability of digital double pulse electrodepositing Ni-HA composite coatings of bioceramics made on 1Crl8Ni9Ti substrate by SEM ,XRD and so on. The results shows that ( 1 ) the HA particles exit in substrate uniformly; (2) XRD result shows that there are HA peaks at 78. 023 ° ,43. 246°and 73. 120°differently; (3) The microhardnees of the composite coatings is increased with the rise of content of HA particles, and on the same conditions the microhardnees value is greater than that of common non-pulse electrodepositing Ni-HA composite coatings of bioceramics. (4) The grain size of digital double pulse electrodepositing Ni-HA composite coatings of bioceramics is much thinner than that of common D. C.
c-Ha-ras BamHI RFLP in human urothelial tumors and point mutations in hot codons

International Nuclear Information System (INIS)

Weismanova, E; Skovraga, M.; Kaluz, S.

1993-01-01

High-molecular weights DNAs from 30 bladder and renal cell carcinomas (RCC) were isolated and the c-Ha-ras the c-Ha-ras gene BamHI RFLP was examined. Amplification of c-Ha-ras with normal localization with regard to the size of alleles was found only in the case. One of the normally localized c-Ha-ras allele termed RCC c-H-ras of a length of about 6.6 kbp was cloned and an oncogene-activating point mutation was identified using two restriction enzymes. After comparison of CfrI and Cfr10I cleavage maps of RCC c-Ha-ras to complete nucleotide sequences of EJ/T24 c-Ha-ras oncogene and its normal counterpart, a point mutation was identified within codon 11 or 12. The use of CfrI and Cfr10I is of value for clinical practice in identification of point mutations in c-Ha-ras PCR product in neoplasia accompanied by somatic mutation of c-Ha-ras. The correlation among c-Ha-ras allele, amplification/loss, presence of point mutation and progression of neoplasia is discussed. (author)
Fabrication and in vitro biocompatibility of biomorphic PLGA/nHA composite scaffolds for bone tissue engineering

International Nuclear Information System (INIS)

Qian, Junmin; Xu, Weijun; Yong, Xueqing; Jin, Xinxia; Zhang, Wei

2014-01-01

In this study, biomorphic poly(DL-lactic-co-glycolic acid)/nano-hydroxyapatite (PLGA/nHA) composite scaffolds were successfully prepared using cane as a template. The porous morphology, phase, compression characteristics and in vitro biocompatibility of the PLGA/nHA composite scaffolds and biomorphic PLGA scaffolds as control were investigated. The results showed that the biomorphic scaffolds preserved the original honeycomb-like architecture of cane and exhibited a bimodal porous structure. The average channel diameter and micropore size of the PLGA/nHA composite scaffolds were 164 ± 52 μm and 13 ± 8 μm, respectively, with a porosity of 89.3 ± 1.4%. The incorporation of nHA into PLGA decreased the degree of crystallinity of PLGA, and significantly improved the compressive modulus of biomorphic scaffolds. The in vitro biocompatibility evaluation with MC3T3-E1 cells demonstrated that the biomorphic PLGA/nHA composite scaffolds could better support cell attachment, proliferation and differentiation than the biomorphic PLGA scaffolds. The localization depth of MC3T3-E1 cells within the channels of the biomorphic PLGA/nHA composite scaffolds could reach approximately 400 μm. The results suggested that the biomorphic PLGA/nHA composite scaffolds were promising candidates for bone tissue engineering. - Highlights: • Novel biomimetic PLGA/nHA composite scaffolds were successfully prepared. • nHA addition improved elastic modulus of PLGA scaffold and decreased its crystallinity. • PLGA/nHA composite scaffolds had better biocompatibility than PLGA scaffolds. • Biomorphic PLGA/nHA composite scaffold had great potential in bone tissue engineering
Fabrication and in vitro biocompatibility of biomorphic PLGA/nHA composite scaffolds for bone tissue engineering

Energy Technology Data Exchange (ETDEWEB)

Qian, Junmin, E-mail: jmqian@mail.xjtu.edu.cn; Xu, Weijun; Yong, Xueqing; Jin, Xinxia; Zhang, Wei

2014-03-01

In this study, biomorphic poly(DL-lactic-co-glycolic acid)/nano-hydroxyapatite (PLGA/nHA) composite scaffolds were successfully prepared using cane as a template. The porous morphology, phase, compression characteristics and in vitro biocompatibility of the PLGA/nHA composite scaffolds and biomorphic PLGA scaffolds as control were investigated. The results showed that the biomorphic scaffolds preserved the original honeycomb-like architecture of cane and exhibited a bimodal porous structure. The average channel diameter and micropore size of the PLGA/nHA composite scaffolds were 164 ± 52 μm and 13 ± 8 μm, respectively, with a porosity of 89.3 ± 1.4%. The incorporation of nHA into PLGA decreased the degree of crystallinity of PLGA, and significantly improved the compressive modulus of biomorphic scaffolds. The in vitro biocompatibility evaluation with MC3T3-E1 cells demonstrated that the biomorphic PLGA/nHA composite scaffolds could better support cell attachment, proliferation and differentiation than the biomorphic PLGA scaffolds. The localization depth of MC3T3-E1 cells within the channels of the biomorphic PLGA/nHA composite scaffolds could reach approximately 400 μm. The results suggested that the biomorphic PLGA/nHA composite scaffolds were promising candidates for bone tissue engineering. - Highlights: • Novel biomimetic PLGA/nHA composite scaffolds were successfully prepared. • nHA addition improved elastic modulus of PLGA scaffold and decreased its crystallinity. • PLGA/nHA composite scaffolds had better biocompatibility than PLGA scaffolds. • Biomorphic PLGA/nHA composite scaffold had great potential in bone tissue engineering.
Inflammation's Association with Metabolic Profiles before and after a Twelve-Week Clinical Trial in Drug-Naïve Patients with Bipolar II Disorder.

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Sheng-Yu Lee

Full Text Available Inflammation is thought to be involved in the pathophysiology of bipolar disorder (BP and metabolic syndrome. Prior studies evaluated the association between metabolic profiles and cytokines only during certain mood states instead of their changes during treatment. We enrolled drug-naïve patients with BP-II and investigated the correlation between changes in mood symptoms and metabolic indices with changes in plasma cytokine levels after 12 weeks of pharmacological treatment. Drug-naïve patients (n = 117 diagnosed with BP-II according to DSM-IV criteria were recruited. Metabolic profiles (cholesterol, triglyceride, HbA1C, fasting serum glucose, body mass index (BMI and plasma cytokines (TNF-α, CRP, IL-6, and TGF-β were measured at baseline and 2, 8, and 12 weeks post-treatment. To adjust within-subject dependence over repeated assessments, multiple linear regressions with generalized estimating equation methods were used. Seventy-six (65.0% patients completed the intervention. Changes in plasma CRP were significantly associated with changes in BMI (P = 1.7E-7 and triglyceride (P = 0.005 levels. Changes in plasma TGF-β1 were significantly associated with changes in BMI (P = 8.2E-6, cholesterol (P = 0.004, and triglyceride (P = 0.006 levels. However, changes in plasma TNF-α and IL-6 were not associated with changes in any of the metabolic indices. Changes in Hamilton Depression Rating Scale scores were significantly associated with changes in IL-6 (P = 0.003 levels; changes in Young Mania Rating Scale scores were significantly associated with changes in CRP (P = 0.006 and TNF-α (P = 0.039 levels. Plasma CRP and TGF-β1 levels were positively correlated with several metabolic indices in BP-II after 12 weeks of pharmacological intervention. We also hypothesize that clinical symptoms are correlated with certain cytokines. These new findings might be important evidence that inflammation is the pathophysiology
Automatización del proceso de transferencia del profiláctico

OpenAIRE

Gastón Vico, Daniel

2006-01-01

Automatización del proceso de transferencia del profiláctico, es un proyecto de diseño de un sistema que transporte los preservativos desde la salida de una máquina de control electrónico de porosidad a una máquina de envasado, sin que se requiera la manipulación de ningún operario. El sistema ha de ser capaz de abastecer la máquina de envasado para así mantener constante la producción de prese4rvativos dela sección. Para ello se ha diseñado un sistema de cintas transportadores que desplaz...
Calpain activation and disturbance of autophagy are induced in cortical neurons in vitro by exposure to HA/β-Ga2O3:Cr3+ nanoparticles.

Science.gov (United States)

Lei, Yu; Wang, Chengkun; Jiang, Quan; Sun, Xiaoyi; Du, Yongzhong; Zhu, Yaofeng; Lu, Yingmei

2018-01-01

The toxicity of engineered nanoparticles remains a concern. The knowledge of biohazards associated with particular nanoparticles is crucial to make this cutting-edge technology more beneficial and safe. Here, we evaluated the toxicity of Ga 2 O 3 nanoparticles (NPs), which are frequently used to enhance the performance of metal catalysts in a variety of catalytic reactions. The potential inflammatory signaling associated with the toxicity of HA/β-Ga 2 O 3 :Cr 3+ NPs in primary cortical neurons was examined. We observed a dose-dependent decrease in cell viability and an increase in apoptosis in neurons following various concentrations (0, 1, 5, 25, 50, 100 µg/ml) of HA/β-Ga 2 O 3 :Cr 3+ NPs treatment. Consistently, constitutively active forms of calcineurin (48 kDa) were significantly elevated in cultured primary cortical neurons, which was consistent with calpain activation indicated by the breakdown products of spectrin. Moreover, HA/β-Ga 2 O 3 :Cr 3+ NPs result in the elevation of LC3-II formation, SQSTM/p62, and Cathepsin B, whereas phosphorylation of CaMKII (Thr286) and Synapsin I (Ser603) were downregulated in the same context. Taken together, these results demonstrate for the first time that calpain activation and a disturbance of autophagy signaling are evoked by exposure to HA/β-Ga 2 O 3 :Cr 3+ NPs, which may contribute to neuronal injury in vitro .

Calpain activation and disturbance of autophagy are induced in cortical neurons in vitro by exposure to HA/β-Ga2O3:Cr3+ nanoparticles

Directory of Open Access Journals (Sweden)

Yu Lei

2018-02-01

Full Text Available The toxicity of engineered nanoparticles remains a concern. The knowledge of biohazards associated with particular nanoparticles is crucial to make this cutting-edge technology more beneficial and safe. Here, we evaluated the toxicity of Ga2O3 nanoparticles (NPs, which are frequently used to enhance the performance of metal catalysts in a variety of catalytic reactions. The potential inflammatory signaling associated with the toxicity of HA/β-Ga2O3:Cr3+ NPs in primary cortical neurons was examined. We observed a dose-dependent decrease in cell viability and an increase in apoptosis in neurons following various concentrations (0, 1, 5, 25, 50, 100 µg/ml of HA/β-Ga2O3:Cr3+ NPs treatment. Consistently, constitutively active forms of calcineurin (48 kDa were significantly elevated in cultured primary cortical neurons, which was consistent with calpain activation indicated by the breakdown products of spectrin. Moreover, HA/β-Ga2O3:Cr3+ NPs result in the elevation of LC3-II formation, SQSTM/p62, and Cathepsin B, whereas phosphorylation of CaMKII (Thr286 and Synapsin I (Ser603 were downregulated in the same context. Taken together, these results demonstrate for the first time that calpain activation and a disturbance of autophagy signaling are evoked by exposure to HA/β-Ga2O3:Cr3+ NPs, which may contribute to neuronal injury in vitro.
Fabrication and Characterization of Collagen-Immobilized Porous PHBV/HA Nano composite Scaffolds for Bone Tissue Engineering

International Nuclear Information System (INIS)

Jin-Young, B.; Zhi-Cai, X.; Giseop, K.; Keun-Byoung, Y.; Soo-Young, P.; Lee, S.P.; Inn-Kyu, K.

2012-01-01

The porous composite scaffolds (PHBV/HA) consisting of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and hydroxyapatite (HA) were fabricated using a hot-press machine and salt-leaching. Collagen (type I) was then immobilized on the surface of the porous PHBV/HA composite scaffolds to improve tissue compatibility. The structure and morphology of the collagen-immobilized composite scaffolds (PHBV/HA/Col) were investigated using a scanning electron microscope (SEM), Fourier transform infrared (FTIR), and electron spectroscopy for chemical analysis (ESCA). The potential of the porous PHBV/HA/Col composite scaffolds for use as a bone scaffold was assessed by an experiment with osteoblast cells (MC3T3-E1) in terms of cell adhesion, proliferation, and differentiation. The results showed that the PHBV/HA/Col composite scaffolds possess better cell adhesion and significantly higher proliferation and differentiation than the PHBV/HA composite scaffolds and the PHBV scaffolds. These results suggest that the PHBV/HA/Col composite scaffolds have a high potential for use in the field of bone regeneration and tissue engineering.
Mishima jo ha kyu

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Matteo Casari

2012-01-01

Full Text Available Abstract – IT La grandezza artistica di Mishima si è espressa attraverso una eterogenea gamma di linguaggi tra i quali il teatro – nella drammaturgia ma anche nella saggistica, regia, recitazione e direzione di compagnia – ha avuto un ruolo di primo piano. Al pari di un uomo di scena Mishima inaugura, a partire dagli anni ’50, un processo di attenzione al proprio corpo come possibile, anzi necessario, veicolo di estrinsecazione etico-estetica tanto da poter istituire l’equazione corpo-teatro quale snodo profondo del suo processo creativo. Il corpo sognato e ottenuto da Mishima attraverso l’addestramento nel kendo, in altre arti marziali e nel body building si conformava ad un ideale estetico di matrice greco-classica assai lontano dal corpo teatrale nipponico. Il corpo come luogo di elaborazione e strumento di espressione autentica da realizzare con impegno, però, lo legano profondamente alle esperienze nascenti – tra gli anni ’50 e ’60 – delle avanguardie teatrali giapponesi. La metafora teatrale è spesso usata nella lettura critica del Mishima uomo e artista con accezione deteriore: un personaggio che dà spettacolo di sé con ripetute provocazioni tra le quali il suicidio del 25 novembre 1970 non sarebbe che l’esempio ultimo e più estremo. La costruzione di sé come personaggio, invece, sembrerebbe corrispondere ad una ben più profonda e meditata necessità di comporre la propria vita in una sapiente messa in scena di classica perfezione: i principi della scansione ritmico formale del jo ha kyu, pilastro teorico del teatro no codificato da Zeami tra XIV e XV secolo, offrono un valido modello di riferimento. Abstract – EN Mishima’s artistic greatness has been expressed through a diverse range of languages among wich theater – in dramaturgy as well as in written essays, as a director, performer and in company direction – played a central role. As a true front-man, Mishima – starting from the 50’s �
Tricalcium phosphate/hydroxyapatite (TCP-HA) bone scaffold as potential candidate for the formation of tissue engineered bone.

Science.gov (United States)

Sulaiman, Shamsul Bin; Keong, Tan Kok; Cheng, Chen Hui; Saim, Aminuddin Bin; Idrus, Ruszymah Bt Hj

2013-06-01

Various materials have been used as scaffolds to suit different demands in tissue engineering. One of the most important criteria is that the scaffold must be biocompatible. This study was carried out to investigate the potential of HA or TCP/HA scaffold seeded with osteogenic induced sheep marrow cells (SMCs) for bone tissue engineering. HA-SMC and TCP/HA-SMC constructs were induced in the osteogenic medium for three weeks prior to implantation in nude mice. The HA-SMC and TCP/HA-SMC constructs were implanted subcutaneously on the dorsum of nude mice on each side of the midline. These constructs were harvested after 8 wk of implantation. Constructs before and after implantation were analyzed through histological staining, scanning electron microscope (SEM) and gene expression analysis. The HA-SMC constructs demonstrated minimal bone formation. TCP/HA-SMC construct showed bone formation eight weeks after implantation. The bone formation started on the surface of the ceramic and proceeded to the centre of the pores. H&E and Alizarin Red staining demonstrated new bone tissue. Gene expression of collagen type 1 increased significantly for both constructs, but more superior for TCP/HA-SMC. SEM results showed the formation of thick collagen fibers encapsulating TCP/HA-SMC more than HA-SMC. Cells attached to both constructs surface proliferated and secreted collagen fibers. The findings suggest that TCP/HA-SMC constructs with better osteogenic potential compared to HA-SMC constructs can be a potential candidate for the formation of tissue engineered bone.
A study on improving mechanical properties of porous HA tissue engineering scaffolds by hot isostatic pressing

International Nuclear Information System (INIS)

Zhao Jing; Xiao Suguang; Lu Xiong; Wang Jianxin; Weng Jie

2006-01-01

Various interconnected porous hydroxyapatite (HA) ceramic scaffolds are universally used to induct the tissue growth for bone repair and replacement, and serve to support the adhesion, transfer, proliferation and differentiation of cells. Impregnation of polyurethane sponges with a ceramic slurry is adopted to produce highly porous HA ceramic scaffolds with a 3D interconnected structure. However, high porosity always accompanies a decrease in the strength of the HA ceramic scaffolds. Therefore, it is significant to improve the strength of the HA ceramic scaffolds with highly interconnected porosity so that they are more suitable in clinical applications. In this work, highly porous HA ceramic scaffolds are first produced by the polymer impregnation approach, and subsequently further sintered by hot isostatic pressing (HIP). The phase composition, macro- and micro-porous structure, sintering and mechanical properties of the porous HA scaffolds are investigated by x-ray diffraction (XRD), scanning electron microscopy (SEM), nanoindentation analysis and compressive test. The experimental results show that the nanohardness and compressive strength of HIP-sintered porous HA ceramics are higher than those of commonly sintered HA scaffolds. The HIP technique can effectively improve the sintering property and densification of porous HA ceramic scaffolds, so inducing an increase in the compression strength
Trehalose improves cell proliferation and dehydration tolerance of human HaCaT cells

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Lee Kyung Eun

2015-01-01

Full Text Available Trehalose is a disaccharide molecule that serves as a natural osmotic regulator in halophilic microorganisms and plants but not in mammals. We observed that human HaCaT cells supplied with trehalose improved cell proliferation and extended viability under dehydration. In HaCaT cells, in response to increasing concentrations of exogenous trehalose, the levels of heat shock protein (HSP 70 increased and matrix metalloproteinase (MMP 1 decreased. Proteome analysis of trehalose-treated HaCaT cells revealed remarkable increases in the levels of proteins involved in cell signaling and the cell cycle, including p21 activated kinase I, Sec I family domain protein and elongation factor G. Moreover, the proteins for cell stress resistance, tryptophan hydroxylase, serine/cysteine proteinase inhibitors and vitamin D receptors were also increased. In addition, the proteins responsible for the maintenance of the cytoskeleton and cellular structures including procollagen-lysine dioxygenase, vinculin and ezrin were increased. Proteomic data revealed that trehalose affected HaCaT cells by inducing the proteins involved in cell proliferation. These results suggest that trehalose improves the proliferation and dehydration tolerance of HaCaT cells by inducing proteins involved in cell growth and dehydration protection.
¿Ha cambiado la esencia de la medicina con los adelantos modernos?

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Pablo Arango Restrepo

2013-01-01

Full Text Available La sociedad a lo largo de los siglos ha tenido cambios progresivos y en la actualidad estos son rápidos e incluyen el ejercicio de la profesión médica, que ha perdido su enfoque inicial en el paciente para centrarse en un interés prin - cipalmente comercial en detrimento de la atención respetuosa al paciente. En el presente artículo se revisa cuál ha sido considerada la esencia de la profesión a lo largo de los siglos, se contrasta con la situación actual y se invita a recuperar el ejercicio médico centrado en el paciente
Mapeamento dos fragmentos de vegetação florestal nativa da bacia hidrográfica do rio Alegre, Espírito Santo, a partir de imagens do satélite IKONOS II Native forest fragmentation mapping of the Alegre river watershed, Espirito Santo State, Brazil, using IKONOS II data

Directory of Open Access Journals (Sweden)

Melchior Carlos do Nascimento

2006-06-01

Full Text Available O estudo teve como objetivos elaborar o mapa de uso da terra e diagnosticar, em nível de paisagem, os fragmentos de vegetação florestal nativa por meio da classificação visual da imagem do satélite IKONOS II. A pesquisa foi desenvolvida na bacia hidrográfica do rio Alegre, situada no extremo sul do Estado do Espírito Santo, Brasil. Foram mapeadas 12 classes de uso da terra, destacando-se 475 fragmentos florestais. As classes cafezal (2.086,2 ha, pastagem (14.130,1 ha e fragmento florestal (2.978,9 ha ocuparam 92,16% (19.195,2 ha da área total da bacia, que é de 20.819,8 ha. A maioria dos fragmentos florestais possui formas fortemente alongadas e área média de 6,3 ha. Também se constatou que a maior parte está sujeita a um elevado nível de perturbação, com 452 e 166 fragmentos florestais vizinhos às classes pastagem e cafezal, respectivamente.The main objective of this study was to create land use and diagnosis maps, at landscape level, of the native forest fragmentation through visual classification using IKONOS II data. The study was conducted in the river Alegre watershed, situated in the south region of State of Espirito Santo, Brazil. Twelve land use classes were mapped, pointing out 475 forest fragments. The classes of coffee plantation (2,086.2 ha, pasture (14,130.1 ha and forest fragmentation (2,978.9 ha occupied 92.16% (19,195.2 ha of the total study area, which was about 20,819.8 ha. The majority of the forest fragments presented strongly elongated shapes, with an average of 6.3 ha. It was also noticed that most of them presented a high level of disturbance, with 452 and 166 forest fragments neighboring the pasture and coffee plantation classes, respectively.
Radial velocity asymmetries from jets with variable velocity profiles

International Nuclear Information System (INIS)

Cerqueira, A. H.; Vasconcelos, M. J.; Velazquez, P. F.; Raga, A. C.; De Colle, F.

2006-01-01

We have computed a set of 3-D numerical simulations of radiatively cooling jets including variabilities in both the ejection direction (precession) and the jet velocity (intermittence), using the Yguazu-a code. In order to investigate the effects of jet rotation on the shape of the line profiles, we also introduce an initial toroidal rotation velocity profile. Since the Yguazu-a code includes an atomic/ionic network, we are able to compute the emission coefficients for several emission lines, and we generate line profiles for the Hα, [O I]λ6300, [S II]λ6716 and [N II]λ6548 lines. Using initial parameters that are suitable for the DG Tau microjet, we show that the computed radial velocity shift for the medium-velocity component of the line profile as a function of distance from the jet axis is strikingly similar for rotating and non-rotating jet models
Synthesis of nano-hydroxyapatite (nHA) from waste mussel shells using a rapid microwave method

Energy Technology Data Exchange (ETDEWEB)

Shavandi, Amin, E-mail: amin.shavandi@postgrad.otago.ac.nz [Department of Food Sciences, University of Otago, Dunedin (New Zealand); Bekhit, Alaa El-Din A. [Department of Food Sciences, University of Otago, Dunedin (New Zealand); Ali, Azam [Department of Applied Sciences, University of Otago, Dunedin (New Zealand); Sun, Zhifa [Department of Physics, University of Otago, Dunedin (New Zealand)

2015-01-15

Nano-crystalline hydroxyapatite (HA, Ca{sub 10}(PO{sub 4}){sub 6}(OH){sub 2}) was produced from waste mussel shells using a rapid microwave irradiation method. Mussel shells were converted to rod like nano-crystalline HA particles of 30–70 nm long using 0.1 M EDTA as a chelating agent for 30 min after an appropriate pre-treatment and an irradiation step in a microwave with a power of 1.1 kW. The produced HA was characterised using X-ray diffraction (XRD), scanning electron microscopy (SEM) coupled with energy-dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), thermo gravimetric analysis (TGA), Fourier transform infrared spectroscopy (FT-IR) and inductively coupled plasma mass spectrometry (ICP-MS) to determine the morphology, particle size, crystal phases, elemental composition and thermal behaviour. Furthermore, to benchmark the synthesized HA obtained from mussel shells, it was compared with a commercially pure HA (Sigma–Aldrich). The thermal analysis showed that the synthesized HA has remarkable heat stability at 1000 °C, and the XRD and FTIR results showed a high purity of the synthesized HA powders. Compared to the conventional hydrothermal treatment, microwave-assisted method has the advantages of an increased rate of HA formation. The obtained HA have potential engineering applications as materials for bone-tissues. - Highlights: • Waste mussel shells were successfully converted to nano sized hydroxyapatite. • Microwave-assisted technique accelerated the conversion process. • The physicochemical properties of the produced hydroxyapatite are reported. • The prepared hydroxyapatite has nano sized particles of less than 100 nm.
The removal of toxic metals from liquid effluents by ion exchange resins. Part II: cadmium(II/ sulphate/Lewatit TP260

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Alguacil, F. J.

2002-10-01

Full Text Available The adsorption of cadmium (II, from aqueous sulphate solutions, on Lewatit TP260 resin has been investigated in batch equilibrium experiments. The influence of pH and temperature on metal adsorption capacity have also been examined. The kinetic performance of the resin has been assesed and the results have been correlated by the pore diffusion model. The resin has been used in mini-columns to study its performance under dynamics conditions. The desorption of metal ion is achieved using sulphuric acid (0.25M and 0.5M.

Se estudia la adsorción de cadmio(II, de disoluciones en medio sulfato, sobre la resina Lewatit TP260. La adsorción del metal se ha investigado en función del pH, la temperatura y el tiempo de contacto con la resina. Los estudios cinéticos permiten correlacionar el proceso de intercambio iónico con el modelo de difusión en poro. Se ha empleado el sistema en mini columnas para evaluar el comportamiento de la resina bajo condiciones dinámicas. La desorción del metal se lleva a cabo con disoluciones de ácido sulfúrico (0,25M y 0,5M.
Measurement of high-beta tokamak pressure profiles with multipoint Thomson scattering

International Nuclear Information System (INIS)

Levinton, F.M.

1983-01-01

A multipoint Thomson-scattering system has been developed to obtain pressure profiles along the major radius of Torus II, a high-beta tokamak. The profiles obtained during the 20 to 25 μs lifetime of the discharge indicates that the plasma has a peak temperature of 80 eV and density of 1.0 x 10 15 cm - 3 . The profiles remain fairly constant during this time until the equilibrium is lost, after which the temperature and density decays to 10 eV and 10 14 cm - 3 very quickly (approx. 1 μs). Experimental results show Torus II has a high-beta ( approx. 10%) equilibrium, with a strong shift of the peak of the pressure profile towards the outside. Numerical results from a 2-D free boundary MHD equilibrium code have obtained equilibria which closely approximate the experimentally measured profiles
CRED Shallow CTD Profiles; Oahu, Main Hawaiian Islands; Cruise: HA1008, Data Date Range: 20101024-20101102 (NODC Accession 0039382).

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
CRED Shallow CTD Profiles; Tutuila, American Samoa; Cruise: HA1201_LEGI, Data Date Range: 20120325-20120326 (NODC Accession 0107470)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
CRED Shallow CTD Profiles; Molokai, Main Hawaiian Islands; Cruise: HA1008, Data Date Range: 20101023-20101104 (NODC Accession 0039382).

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
CRED Shallow CTD Profiles; Niihau, Main Hawaiian Islands; Cruise: HA1008, Data Date Range: 20101029-20101101 (NODC Accession 0039382).

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
CRED Shallow CTD Profiles; Maui, Main Hawaiian Islands; Cruise: HA1008, Data Date Range: 20101015-20101020 (NODC Accession 0039382).

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
Detonation Wave Profile

Energy Technology Data Exchange (ETDEWEB)

Menikoff, Ralph [Los Alamos National Laboratory

2015-12-14

The Zel’dovich-von Neumann-Doering (ZND) profile of a detonation wave is derived. Two basic assumptions are required: i. An equation of state (EOS) for a partly burned explosive; P(V, e, λ). ii. A burn rate for the reaction progress variable; d/dt λ = R(V, e, λ). For a steady planar detonation wave the reactive flow PDEs can be reduced to ODEs. The detonation wave profile can be determined from an ODE plus algebraic equations for points on the partly burned detonation loci with a specified wave speed. Furthermore, for the CJ detonation speed the end of the reaction zone is sonic. A solution to the reactive flow equations can be constructed with a rarefaction wave following the detonation wave profile. This corresponds to an underdriven detonation wave, and the rarefaction is know as a Taylor wave.
Effects of Sesame Oil on Blood Glucose and Lipid Profile in Type II Diabetic Patients Referring to The Yazd Diabetes Research Center.

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F Hoseini

2008-07-01

Full Text Available Introduction: Type II Diabetes is one of the most prevalent endocrine diseases in the world that results from a combination of insulin resistance and ß-cell failure. Regarding importance of nutritional factors in management of diabetes, this study was designed to explore the effect of sesame oil on blood glucose and lipid profile in type II diabetic patients at Yazd Diabetes Research Center in 2007. Methods: A quasi-experimental study was conducted on 25 patients with type II diabetes mellitus (age: 51.5±6.28y; BMI:27.3±3kg/m2; disease duration:7.08±5.03y; Fasting blood glucose level: 181±51.9mg/dl. Subjects received 30 g/day sesame oil for 6 weeks. Sesame oil was supplied to the patients, who were instructed to use it in place of other cooking oils for 42 days. Plasma glucose, glycated hemoglobin (HbA1c, lipid profiles [Total cholesterol (TC, low-density lipoprotein cholesterol (LDL-C, high-density lipoprotein cholesterol (HDL-C and triglycerides (TG] were measured at baseline and after 45 days of sesame oil substitution. 24 hours dietary recalls were obtained at the start , middle and end of study. Data was analyzed using analysis of variance with repeated measures and paired t-test. Results: Following 42 days intake of sesame oil, there were significant decrease in FBS (181±51.93 vs 154±39.65 mg/dl, HbA1c (9.64 ± 2 vs 8.4 ± 1.74 percent, TC (226.68 ± 31.4 vs 199.8 ± 37.87 mg/dl, LDL-c (123.9 ± 34.56 vs 95.53 ± 32.54 mg/dl compared to pre-treatment values. (P <0.05 . Blood TG level decreased after intake of sesame oil but this difference was not significant (P=0.2.Also, the changes of HDL-c levels were not significant (P=0.1. Conclusion: Sesame oil consumption results in considerable decrease in blood sugar, HbA1c and blood lipid levels (TC and LDL-C in type II diabetics.
Fatty Acid Profile of Sunshine Bass: II. Profile Change Differs Among Fillet Lipid Classes.

Science.gov (United States)

Trushenski, Jesse T; Lewis, Heidi A; Kohler, Christopher C

2008-07-01

Fatty acid (FA) profile of fish tissue mirrors dietary FA profile and changes in a time-dependent manner following a change in dietary FA composition. To determine whether FA profile change varies among lipid classes, we evaluated the FA composition of fillet cholesteryl esters (CE), phospholipids (PL), and triacylglycerols (TAG) of sunshine bass (SB, Morone chrysops x M. saxatilis) raised on feeds containing fish oil or 50:50 blend of fish oil and coconut, grapeseed, linseed, or poultry oil, with or without implementation of a finishing period (100% FO feed) prior to harvest. Each lipid class was associated with a generalized FA signature, irrespective of nutritional history: fillet PL was comprised largely of saturated FA (SFA), long-chain polyunsaturated FA (LC-PUFA), and total n-3 FA; fillet TAG was higher in MC-PUFA and total n-6 FA; and fillet CE was highest in monounsaturated FA (MUFA). Neutral lipids reflected dietary composition in a near-direct fashion; conversely, PL showed evidence of selectivity for MC- and LC-PUFA. Shorter-chain SFA were not strongly reflected within any lipid fraction, even when dietary availability was high, suggesting catabolism of these FA. FA metabolism in SB is apparently characterized by a division between saturated and unsaturated FA, whereby LC-PUFA are preferentially incorporated into tissues and SFA are preferentially oxidized for energy production. We demonstrated provision of SFA in grow-out feeds for SB, instead MC-PUFA which compete for tissue deposition, meets energy demands and allows for maximum inclusion of LC-PUFA within fillet lipids.

El perfil profesional de los formadores de formación continua en España. [The professional profile of trainers working in continuous training in Spain].

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Mamaqi, Xhevrie

2011-04-01

Full Text Available The aim of this study is to analyze and describe the professional profile of trainers working in continuous training in Spain. For this purpose we have developed a structured questionnaire was applied in person to a sample of 606 instructors nationwide. The questionnaire has provided information on aspects such as the socio-occupational status of instructors, their degree of professionalism, and the importance of professional skills. The information gathered has been analyzed by multivariate methods to determine the dominant professional profiles. The quantitative analysis includes the Categorical of Principal Components Analysis (CATPCA to analyze the skills and capabilities of the trainer and cluster analysis in two stages to get the profiles. Four dominant profiles have been deduced by the cluster analysis. The occupational variables, professional experience profiles and competences/skills produce the major discrepancies between the four profiles. El objetivo de este trabajo es analizar y describir los perfiles profesionales del formador de formación continua en España. Con este propósito se ha elaborado un cuestionario estructurado que se ha aplicado de forma presencial a una muestra de 606 formadores a nivel nacional. El cuestionario ha proporcionado información sobre aspectos como el estatus socio-laboral del formador, el grado de profesionalización y la importancia de las competencias profesionales de los formadores. La información recabada ha sido analizada mediante métodos multivariantes para determinar los perfiles profesionales dominantes. El análisis cuantitativo incluye el Análisis de Componentes Principales Categóricos (CATPCA para analizar las competencias y capacidades del formador y el análisis Cluster en Dos Fases para obtener los perfiles. De los resultados obtenidos se han deducido cuatro perfiles profesionales dominantes siendo las variables ocupacionales, experiencia profesional y de las capacidades las que mayor
Summary of results from velocity profile tests and wastage tests in support of LLTR series II test A-4

International Nuclear Information System (INIS)

Greene, D.A.

1981-01-01

The following conclusions were drawn from the experimental program conducted in support of LLTR (Large Leak Test Rig) Series II Test A-4: Fabrication technique for making precise slits was developed. Wastage boundary agrees with velocity profile boundary. Circumferential slit angles would have to be 120 0 to ensure adequate coverage of adjacent tubes. 120 0 circumferential slit weakens tubes such that maintaining desired slit dimensions for LLTI application is not considered practical. Use of intermittent slit geometry would be required. 120 0 slits, precisely machined and precisely aligned with target tubes, produced different penetration rates on adjacent tubes. Production of simultaneous failures in LLTI with 120 0 slit or simulated interrupted slit is not considered credible
Profiling MHC II immunopeptidome of blood-stage malaria reveals that cDC1 control the functionality of parasite-specific CD4 T cells.

Science.gov (United States)

Draheim, Marion; Wlodarczyk, Myriam F; Crozat, Karine; Saliou, Jean-Michel; Alayi, Tchilabalo Dilezitoko; Tomavo, Stanislas; Hassan, Ali; Salvioni, Anna; Demarta-Gatsi, Claudia; Sidney, John; Sette, Alessandro; Dalod, Marc; Berry, Antoine; Silvie, Olivier; Blanchard, Nicolas

2017-11-01

In malaria, CD4 Th1 and T follicular helper (T FH ) cells are important for controlling parasite growth, but Th1 cells also contribute to immunopathology. Moreover, various regulatory CD4 T-cell subsets are critical to hamper pathology. Yet the antigen-presenting cells controlling Th functionality, as well as the antigens recognized by CD4 T cells, are largely unknown. Here, we characterize the MHC II immunopeptidome presented by DC during blood-stage malaria in mice. We establish the immunodominance hierarchy of 14 MHC II ligands derived from conserved parasite proteins. Immunodominance is shaped differently whether blood stage is preceded or not by liver stage, but the same ETRAMP-specific dominant response develops in both contexts. In naïve mice and at the onset of cerebral malaria, CD8α + dendritic cells (cDC1) are superior to other DC subsets for MHC II presentation of the ETRAMP epitope. Using in vivo depletion of cDC1, we show that cDC1 promote parasite-specific Th1 cells and inhibit the development of IL-10 + CD4 T cells. This work profiles the P. berghei blood-stage MHC II immunopeptidome, highlights the potency of cDC1 to present malaria antigens on MHC II, and reveals a major role for cDC1 in regulating malaria-specific CD4 T-cell responses. © 2017 The Authors. Published under the terms of the CC BY 4.0 license.
Casein Kinase 1α Mediates the Degradation of Receptors for Type I and Type II Interferons Caused by Hemagglutinin of Influenza A Virus.

Science.gov (United States)

Xia, Chuan; Wolf, Jennifer J; Vijayan, Madhuvanthi; Studstill, Caleb J; Ma, Wenjun; Hahm, Bumsuk

2018-04-01

Although influenza A virus (IAV) evades cellular defense systems to effectively propagate in the host, the viral immune-evasive mechanisms are incompletely understood. Our recent data showed that hemagglutinin (HA) of IAV induces degradation of type I IFN receptor 1 (IFNAR1). Here, we demonstrate that IAV HA induces degradation of type II IFN (IFN-γ) receptor 1 (IFNGR1), as well as IFNAR1, via casein kinase 1α (CK1α), resulting in the impairment of cellular responsiveness to both type I and II IFNs. IAV infection or transient HA expression induced degradation of both IFNGR1 and IFNAR1, whereas HA gene-deficient IAV failed to downregulate the receptors. IAV HA caused the phosphorylation and ubiquitination of IFNGR1, leading to the lysosome-dependent degradation of IFNGR1. Influenza viral HA strongly decreased cellular sensitivity to type II IFNs, as it suppressed the activation of STAT1 and the induction of IFN-γ-stimulated genes in response to exogenously supplied recombinant IFN-γ. Importantly, CK1α, but not p38 MAP kinase or protein kinase D2, was proven to be critical for HA-induced degradation of both IFNGR1 and IFNAR1. Pharmacologic inhibition of CK1α or small interfering RNA (siRNA)-based knockdown of CK1α repressed the degradation processes of both IFNGR1 and IFNAR1 triggered by IAV infection. Further, CK1α was shown to be pivotal for proficient replication of IAV. Collectively, the results suggest that IAV HA induces degradation of IFN receptors via CK1α, creating conditions favorable for viral propagation. Therefore, the study uncovers a new immune-evasive pathway of influenza virus. IMPORTANCE Influenza A virus (IAV) remains a grave threat to humans, causing seasonal and pandemic influenza. Upon infection, innate and adaptive immunity, such as the interferon (IFN) response, is induced to protect hosts against IAV infection. However, IAV seems to be equipped with tactics to evade the IFN-mediated antiviral responses, although the detailed
Identified ankle extensor and flexor motoneurons display different firing profiles in the neonatal rat

DEFF Research Database (Denmark)

Cotel, Florence; Antri, Myriam; Barthe, Jean-Yves

2009-01-01

population of flexor motoneurons solely exhibited the type II profile, characterized by a frequency-current (F-I) relationship with a clockwise hysteresis. In contrast, in addition to this type II profile, the other three profiles of repetitive firing (type I, III and IV) were observed in extensor...... postnatal development, a significant part of the population of extensor motoneurons, but not flexors, are able to produce self-sustained discharges known to involve the activation of persistent inward currents....
PCL-HA microscaffolds for in vitro modular bone tissue engineering.

Science.gov (United States)

Totaro, Alessandra; Salerno, Aurelio; Imparato, Giorgia; Domingo, Concepción; Urciuolo, Francesco; Netti, Paolo Antonio

2017-06-01

The evolution of microscaffolds and bone-bioactive surfaces is a pivotal point in modular bone tissue engineering. In this study, the design and fabrication of porous polycaprolactone (PCL) microscaffolds functionalized with hydroxyapatite (HA) nanoparticles by means of a bio-safe and versatile thermally-induced phase separation process is reported. The ability of the as-prepared nanocomposite microscaffolds to support the adhesion, growth and osteogenic differentiation of human mesenchymal stem cells (hMSCs) in standard and osteogenic media and using dynamic seeding/culture conditions was investigated. The obtained results demonstrated that the PCL-HA nanocomposite microparticles had an enhanced interaction with hMSCs and induced their osteogenic differentiation, even without the exogenous addition of osteogenic factors. In particular, calcium deposition, alizarin red assay, histological analysis, osteogenic gene expression and collagen I secretion were assessed. The results of these tests demonstrated the formation of bone microtissue precursors after 28 days of dynamic culture. These findings suggest that PCL-HA nanocomposite microparticles represent an excellent platform for in vitro modular bone tissue engineering. Copyright © 2015 John Wiley & Sons, Ltd. Copyright © 2015 John Wiley & Sons, Ltd.
Gene expression profiling associated with angiotensin II type 2 receptor-induced apoptosis in human prostate cancer cells.

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Nana Pei

Full Text Available Increased expression of angiotensin II type 2 receptor (AT2R induces apoptosis in numerous tumor cell lines, with either Angiotensin II-dependent or Angiotensin II-independent regulation, but its molecular mechanism remains poorly understood. Here, we used PCR Array analysis to determine the gene and microRNA expression profiles in human prostate cancer cell lines transduced with AT2R recombinant adenovirus. Our results demonstrated that AT2R over expression leads to up-regulation of 6 apoptosis-related genes (TRAIL-R2, BAG3, BNIPI, HRK, Gadd45a, TP53BP2, 2 cytokine genes (IL6 and IL8 and 1 microRNA, and down-regulation of 1 apoptosis-related gene TNFSF10 and 2 cytokine genes (BMP6, BMP7 in transduced DU145 cells. HRK was identified as an up-regulated gene in AT2R-transduced PC-3 cells by real-time RT-PCR. Next, we utilized siRNAs to silence the up-regulated genes to further determine their roles on AT2R overexpression mediated apoptosis. The results showed downregulation of Gadd45a reduced the apoptotic effect by ∼30% in DU145 cells, downregulation of HRK reduced AT2R-mediated apoptosis by more than 50% in PC-3 cells, while downregulation of TRAIL-R2 enhanced AT2R-mediated apoptosis more than 4 times in DU145 cells. We also found that the effects on AT2R-mediated apoptosis caused by downregulation of Gadd45a, TRAIL-R2 and HRK were independent in activation of p38 MAPK, p44/42 MAPK and p53. Taken together, our results demonstrated that TRAIL-R2, Gadd45a and HRK may be novel target genes for further study of the mechanism of AT2R-mediated apoptosis in prostate cancer cells.
Crystallization and preliminary X-ray analysis of the HA3 component of Clostridium botulinum type C progenitor toxin

Energy Technology Data Exchange (ETDEWEB)

Nakamura, Toshio; Tonozuka, Takashi; Kotani, Mao; Obata, Kanae [Department of Applied Biological Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509 (Japan); Oguma, Keiji [Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8558 (Japan); Nishikawa, Atsushi, E-mail: nishikaw@cc.tuat.ac.jp [Department of Applied Biological Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509 (Japan); CREST, Japan Science and Technology Agency (Japan)

2007-12-01

HA3, a 70 kDa haemagglutinating protein, is a precursor form of HA3a and HA3b, the subcomponents of Clostridium botulinum type C 16S progenitor toxin. In this report, recombinant HA3 protein was overexpressed in Escherichia coli, purified and crystallized. HA3, a 70 kDa haemagglutinating protein, is a precursor form of HA3a and HA3b, the subcomponents of Clostridium botulinum type C 16S progenitor toxin. In this report, recombinant HA3 protein was overexpressed in Escherichia coli, purified and crystallized. Diffraction data were collected to 2.6 Å resolution and the crystal belonged to the hexagonal space group P6{sub 3}. Matthews coefficient and self-rotation function calculations indicate that there is probably one molecule of HA3 in the asymmetric unit. A search for heavy-atom derivatives has been undertaken.
Risk profiles of personality traits for suicidality among mood disorder patients and community controls.

Science.gov (United States)

Su, M-H; Chen, H-C; Lu, M-L; Feng, J; Chen, I-M; Wu, C-S; Chang, S-W; Kuo, P-H

2018-01-01

To examine the associations between personality traits and suicidal ideation (SI) and attempt (SA) in mood disorder patients and community controls. We recruited 365 bipolar, 296 major depressive disorder patients, and 315 community controls to assess their lifetime suicidality. Participants filled out self-reported personality questionnaires to collect data of personality traits, including novelty seeking (NS), harm avoidance (HA), extraversion (E), and neuroticism (N). We used logistic regression models adjusted for diagnoses to analyze combinational effects of personality traits on the risk of suicide. Additionally, radar charts display personality profiles for suicidal behaviours by groups. All personality traits were associated with the risk of suicidality with various effect size, except for E that showed protective effect. High N or HA had prominent and independent risk effects on SI and SA. Combinations of high N and low E, or high HA and NS were the risk personality profiles for suicidality. Higher N scores further distinguished SA from SI in mood disorder patients. Introvert personality traits showed independent risk effects on suicidality regardless of diagnosis status. Among high-risk individuals with suicidal thoughts, higher neuroticism tendency is further associated with increased risk of suicide attempt. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
Characterization of the anti-factor VIII immunoglobulin profile in patients with hemophilia A by use of a fluorescence-based immunoassay

Science.gov (United States)

Boylan, Brian; Rice, Anne S.; Dunn, Amy L.; Tarantino, Michael D.; Brettler, Doreen B.; Barrett, John C.; Miller, Connie H.

2015-01-01

Summary Background The development of neutralizing antibodies, referred to as inhibitors, against factor VIII (FVIII) is a major complication associated with FVIII infusion therapy for the treatment of hemophilia A (HA). Previous studies have shown that a subset of HA patients and a low percentage of healthy individuals harbor non-neutralizing anti-FVIII antibodies that do not elicit the clinical manifestations associated with inhibitor development. Objective Assess HA patients' anti-FVIII antibody profiles as potential predictors of clinical outcomes. Methods A fluorescence immunoassay (FLI) was used to detect anti-FVIII antibodies in 491 samples from 371 HA patients. Results Assessments of antibody profiles showed that the presence of anti-FVIII IgG1, IgG2, or IgG4 correlated qualitatively and quantitatively with the presence of a FVIII inhibitor as reported by the Nijmegen-Bethesda assay (NBA). Forty-eight patients with a negative inhibitor history contributed serial samples to the study, including seven patients who had negative NBA titers initially and later converted to NBA-positive. The FLI detected anti-FVIII IgG1 in five of those seven patients prior to their conversion to NBA-positive. Five of 15 serial-sample patients who had a negative inhibitor history and a positive anti-FVIII IgG1 later developed an inhibitor, compared to 2 of 33 patients with a negative inhibitor history without anti-FVIII IgG1. Conclusions These data provide a rationale for future studies designed both to monitor the dynamics of anti-FVIII antibody profiles in HA patients as a potential predictor of future inhibitor development and to assess the value of the anti-FVIII FLI as a supplement to traditional inhibitor testing. PMID:25354263
ALP gene expression in cDNA samples from bone tissue engineering using a HA/TCP/Chitosan scaffold

Science.gov (United States)

Stephanie, N.; Katarina, H.; Amir, L. R.; Gunawan, H. A.

2017-08-01

This study examined the potential use of hydroxyapatite (HA)/tricalcium phosphate (TCP)/Chitosan as a bone tissue engineering scaffold. The potential for using HA/TCP/chitosan as a scaffold was analyzed by measuring expression of the ALP osteogenic gene in cDNA from bone biopsies from four Macaque nemestrina. Experimental conditions included control (untreated), treatment with HA/TCP 70:30, HA/TCP 50:50, and HA/TCP/chitosan. cDNA samples were measured quantitively with Real-Time PCR (qPCR) and semi-quantitively by gel electrophoresis. There were no significant differences in ALP gene expression between treatment subjects after two weeks, but the HA/TCP/chitosan treatment gave the highest level of expression after four weeks. The scaffold using the HA/TCP/chitosan combination induced a higher level of expression of the osteogenic gene ALP than did scaffold without chitosan.
Oxidative Stress Posttranslationally Regulates the Expression of Ha-Ras and Ki-Ras in Cultured Astrocytes

Directory of Open Access Journals (Sweden)

Samantha Messina

2012-01-01

Full Text Available Addition of hydrogen peroxide to cultured astrocytes induced a rapid and transient increase in the expression of Ha-Ras and Ki-Ras. Pull-down experiments with the GTP-Ras-binding domain of Raf-1 showed that oxidative stress substantially increased the activation of Ha-Ras, whereas a putative farnesylated activated form of Ki-Ras was only slightly increased. The increase in both Ha-Ras and Ki-Ras was insensitive to the protein synthesis inhibitor, cycloheximide, and was occluded by the proteasomal inhibitor, MG-132. In addition, exposure to hydrogen peroxide reduced the levels of ubiquitinated Ras protein, indicating that oxidative stress leads to a reduced degradation of both isoforms through the ubiquitin/proteasome pathway. Indeed, the late reduction in Ha-Ras and Ki-Ras was due to a recovery of proteasomal degradation because it was sensitive to MG-132. The late reduction of Ha-Ras levels was abrogated by compound PD98059, which inhibits the MAP kinase pathway, whereas the late reduction of Ki-Ras was unaffected by PD98059. We conclude that oxidative stress differentially regulates the expression of Ha-Ras and Ki-Ras in cultured astrocytes, and that activation of the MAP kinase pathway by oxidative stress itself or by additional factors may act as a fail-safe mechanism limiting a sustained expression of the potentially detrimental Ha-Ras.
Characterization of growth and Oryctes rhinoceros nudivirus production in attached cultures of the DSIR-HA-1179 coleopteran insect cell line.

Science.gov (United States)

Pushparajan, Charlotte; Claus, Juan Daniel; Marshall, Sean David Goldie; Visnovsky, Gabriel

2013-12-01

The DSIR-HA-1179 coleopteran cell line is a susceptible and permissive host to the Oryctes rhinoceros nudivirus (OrNV), which has been used as a biocontrol agent against the coconut rhinoceros beetle (Oryctes rhinoceros); a pest of palms in the Asia-Pacific region. However, little is known about growth and metabolism of this cell line, knowledge of which is necessary to develop an in vitro large-scale OrNV production process. The strong anchorage-dependent characteristics of the cell line, its particular fragility and its tendency to form dense clumps when manipulated, are the most likely reasons that have precluded further development of the cell line. In order to characterize DSIR-HA-1179 cells, there was first a need for a reliable technique to count the cells. A homogenous cell suspension suitable for enumeration could be produced by treatment with TrypLE Express™ with optimum mean time for cell release calculated as 30 min. The cell line was adapted to grow in four serum-supplemented culture media namely TC-100, IPL-41, Sf-900 II and Sf-900 III and cell growth, glucose consumption, lactate and ammonia production were assessed from static-batch cultures. The maximum viable cell density was reached in Sf-900 II (17.9 × 10(5) cells/ml), with the maximum specific growth rate observed in this culture medium as well (0.0074 h(-1)). Higher production of OrNV was observed in IPL-41 and TC-100 (4.1 × 10(7) TCID50/ml) than in cultures infected in Sf-900 III (2.0 × 10(7) TCID50/ml) and Sf-900 II (1.4 × 10(7) TCID50/ml). At the end of the growth period, glucose was completely consumed in cultures grown in TC-100, while remained in excess in the other three culture media. The cell line produced lactate and ammonia to very low levels in the TC-100 culture medium which is a promising aspect for its cultivation at large-scale.
The use of acetone to enhance the infiltration of HA nanoparticles into a demineralized dentin collagen matrix.

Science.gov (United States)

Besinis, Alexandros; van Noort, Richard; Martin, Nicolas

2016-03-01

This study investigates the role of acetone, as a carrier for nano-hydroxyapatite (nano-HA) in solution, to enhance the infiltration of fully demineralized dentin with HA nanoparticles (NPs). Dentin specimens were fully demineralized and subsequently infiltrated with two types of water-based nano-HA solutions (one containing acetone and one without). Characterization of the dentin surfaces and nano-HA particles was performed using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The surface wettability and infiltration capacity of the nano-HA solutions were quantified by means of contact angle measurements and energy dispersive X-ray spectroscopy (EDS), respectively. Contact angle measurements were taken at baseline and repeated at regular intervals to assess the effect of acetone. The P and Ca levels of infiltrated dentin specimens were measured and compared to sound dentin and non-infiltrated controls. The presence of acetone resulted in an eight-fold decrease in the contact angles of the nano-HA solutions recorded on the surface of demineralized dentin compared to nano-HA solutions without acetone (one-way ANOVA, pacetone. Infiltration of demineralized dentin with the nano-HA solution containing acetone restored the lost mineral content by 50%, whereas the mean mineralization values for P and Ca in dentin treated with the acetone-free nano-HA solution were less than 6%. Acetone was shown to act as a vehicle to enhance the capacity to infiltrate demineralized dentin with HA NPs. The successful infiltration of dentin collagen with HA NPs provides a suitable scaffold, whereby the infiltrated HA NPs have the potential to act as seeds that may initiate heterogenous mineral growth when exposed to an appropriate mineral-rich environment. Copyright © 2015 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
Ha-DEF1, a sunflower defensin, induces cell death in Orobanche parasitic plants.

Science.gov (United States)

de Zélicourt, Axel; Letousey, Patricia; Thoiron, Séverine; Campion, Claire; Simoneau, Philippe; Elmorjani, Khalil; Marion, Didier; Simier, Philippe; Delavault, Philippe

2007-08-01

Plant defensins are small basic peptides of 5-10 kDa and most of them exhibit antifungal activity. In a sunflower resistant to broomrape, among the three defensin encoding cDNA identified, SF18, SD2 and HaDef1, only HaDef1 presented a preferential root expression pattern and was induced upon infection by the root parasitic plant Orobanche cumana. The amino acid sequence deduced from HaDef1 coding sequence was composed of an endoplasmic reticulum signal sequence of 28 amino acids, a standard defensin domain of 50 amino-acid residues and an unusual C-terminal domain of 30 amino acids with a net positive charge. A 5.8 kDa recombinant mature Ha-DEF1 corresponding to the defensin domain was produced in Escherichia coli and was purified by means of a two-step chromatography procedure, Immobilized Metal Affinity Chromatography (IMAC) and Ion Exchange Chromatography. Investigation of in vitro antifungal activity of Ha-DEF1 showed a strong inhibition on Saccharomyces cerevisiae growth linked to a membrane permeabilization, and a morphogenetic activity on Alternaria brassicicola germ tube development, as already reported for some other plant defensins. Bioassays also revealed that Ha-DEF1 rapidly induced browning symptoms at the radicle apex of Orobanche seedlings but not of another parasitic plant, Striga hermonthica, nor of Arabidopsis thaliana. FDA vital staining showed that these browning areas corresponded to dead cells. These results demonstrate for the first time a lethal effect of defensins on plant cells. The potent mode of action of defensin in Orobanche cell death and the possible involvement in sunflower resistance are discussed.
Designing, preparing and evaluation of novel HA/Ti composite coating for endodontic dental implant

Directory of Open Access Journals (Sweden)

Fathi MH.

2002-08-01

Full Text Available Nowadays, application of implants as a new method for replacing extracted teeth have been improved. So, many researches have been performed for improving the characteristics of implants. The aim of this study was to design and produce a desired coating in order to obtaining two goals including; improvement of the corrosion behavior of metallic endodontic implant and the bone osseointegration simultaneously. Stainless steel 316L (SS, cobalt-chromium alloy (Vit and commercial pure titanium (cpTi were chosen as metallic substrates and hydroxyapatite coating (HAC were performed by plasma-spraying (PS process on three different substrates. A novel double layer Hydroxyapatite/Titanium (HA/Ti composite coating composed of a HA top layer and a Ti under layer was prepared using PS and physical vapor deposition (PVD process respectively on SS. Structural characterization techniques including XRD, SEM and EDX were utilized to investigate the microstructure, morpholgy and crystallinity of the coatings. Electrochemical potentiodynamic tests were performed in physiological solutions in order to determine and compare the corrosion behavior of the coated and uncoated specimens behavior as an indication of biocmpatibility. Results indicated that the cpTi possesses the highest and SS the lowest corrosion resistance (highest corrosion current density between uncoated substrates. This trend was independent to the type of physiological environment. The HA coating decreased the corrosion current density of HA coated metallic implants but did not change that trend. HAC acted as a mechanical barrier on the metallic substrate but could not prevent the interaction between metallic substrate and environment completely. The HA/Ti composite coating improved the corrosion behavior of SS. The corrosion current density of HA/Ti coated SS decreased and was exactly similar to single HA coated cpTi in physiological solutions. The results indicated that HA/Ti composite coated SS
Microstructure and mechanical properties of plasma sprayed HA/YSZ/Ti-6Al-4V composite coatings.

Science.gov (United States)

Khor, K A; Gu, Y W; Pan, D; Cheang, P

2004-08-01

Plasma sprayed hydroxyapatite (HA) coatings on titanium alloy substrate have been used extensively due to their excellent biocompatibility and osteoconductivity. However, the erratic bond strength between HA and Ti alloy has raised concern over the long-term reliability of the implant. In this paper, HA/yttria stabilized zirconia (YSZ)/Ti-6Al-4V composite coatings that possess superior mechanical properties to conventional plasma sprayed HA coatings were developed. Ti-6Al-4V powders coated with fine YSZ and HA particles were prepared through a unique ceramic slurry mixing method. The so-formed composite powder was employed as feedstock for plasma spraying of the HA/YSZ/Ti-6Al-4V coatings. The influence of net plasma energy, plasma spray standoff distance, and post-spray heat treatment on microstructure, phase composition and mechanical properties were investigated. Results showed that coatings prepared with the optimum plasma sprayed condition showed a well-defined splat structure. HA/YSZ/Ti-6Al-4V solid solution was formed during plasma spraying which was beneficial for the improvement of mechanical properties. There was no evidence of Ti oxidation from the successful processing of YSZ and HA coated Ti-6Al-4V composite powders. Small amount of CaO apart from HA, ZrO(2) and Ti was present in the composite coatings. The microhardness, Young's modulus, fracture toughness, and bond strength increased significantly with the addition of YSZ. Post-spray heat treatment at 600 degrees C and 700 degrees C for up to 12h was found to further improve the mechanical properties of coatings. After the post-spray heat treatment, 17.6% increment in Young's modulus (E) and 16.3% increment in Vicker's hardness were achieved. The strengthening mechanisms of HA/YSZ/Ti-6Al-4V composite coatings were related to the dispersion strengthening by homogeneous distribution of YSZ particles in the matrix, the good mechanical properties of Ti-6Al-4V and the formation of solid solution among HA
Estudio de reacciones de oxidación catalizadas por complejos biomiméticos de Fe (II), Fe (III) y Mn (III)

OpenAIRE

Clemente Tejeda, David

2015-01-01

[ES]Se ha desarrollado una metodología económicamente asequible y medioambientalmente sostenible para la oxidación catalítica de sustratos orgánicos con interés preparativo, utilizando para ello sistemas biomiméticos de Fe(II), Fe(III) y Mn(III). En primer lugar se ha llevado a cabo la síntesis de los complejos metálicos catalizadores, optimizando los procedimientos descritos en la bibliografía precedente. Asimismo se ha llevado a cabo un análisis exhaustivo de todas las variables implicadas ...
Fragrance chemicals lyral and lilial decrease viability of HaCat cells' by increasing free radical production and lowering intracellular ATP level: protection by antioxidants.

Science.gov (United States)

Usta, Julnar; Hachem, Yassmine; El-Rifai, Omar; Bou-Moughlabey, Yolla; Echtay, Karim; Griffiths, David; Nakkash-Chmaisse, Hania; Makki, Rajaa Fakhoury

2013-02-01

We investigate in this study the biochemical effects on cells in culture of two commonly used fragrance chemicals: lyral and lilial. Whereas both chemicals exerted a significant effect on primary keratinocyte(s), HaCat cells, no effect was obtained with any of HepG2, Hek293, Caco2, NIH3T3, and MCF7 cells. Lyral and lilial: (a) decreased the viability of HaCat cells with a 50% cell death at 100 and 60 nM respectively; (b) decreased significantly in a dose dependant manner the intracellular ATP level following 12-h of treatment; (c) inhibited complexes I and II of electron transport chain in liver sub-mitochondrial particles; and (d) increased reactive oxygen species generation that was reversed by N-acetyl cysteine and trolox and the natural antioxidant lipoic acid, without influencing the level of free and/or oxidized glutathione. Lipoic acid protected HaCat cells against the decrease in viability induced by either compound. Dehydrogenation of lyral and lilial produce α,β-unsaturated aldehydes, that reacts with lipoic acid requiring proteins resulting in their inhibition. We propose lyral and lilial as toxic to mitochondria that have a direct effect on electron transport chain, increase ROS production, derange mitochondrial membrane potential, and decrease cellular ATP level, leading thus to cell death. Copyright © 2012 Elsevier Ltd. All rights reserved.
Functionalized PCL/HA nanocomposites as microporous membranes for bone regeneration

Energy Technology Data Exchange (ETDEWEB)

Basile, Maria Assunta; Gomez d' Ayala, Giovanna; Malinconico, Mario [Institute for Polymers, Composites and Biomaterials, CNR, Via Campi Flegrei 34, Pozzuoli (Naples) (Italy); Laurienzo, Paola, E-mail: paola.laurienzo@ipcb.cnr.it [Institute for Polymers, Composites and Biomaterials, CNR, Via Campi Flegrei 34, Pozzuoli (Naples) (Italy); Coudane, Jean; Nottelet, Benjamin [Institut des Biomolécules Max Mousseron (IBMM), Artificial Biopolymers Group, CNRS UMR 5247, University of Montpellier 1, Faculty of Pharmacy, 15 Av. C. Flahault, Montpellier 34093 (France); Ragione, Fulvio Della [Department of Biochemistry and Biophysics, Second University of Naples, Via L. De Crecchio 7, Naples (Italy); Oliva, Adriana, E-mail: adriana.oliva@unina2.it [Department of Biochemistry and Biophysics, Second University of Naples, Via L. De Crecchio 7, Naples (Italy)

2015-03-01

In the present work, microporous membranes based on poly(ε-caprolactone) (PCL) and PCL functionalized with amine (PCL-DMAEA) or anhydride groups (PCL-MAGMA) were realized by solvent–non solvent phase inversion and proposed for use in Guided Tissue Regeneration (GTR). Nanowhiskers of hydroxyapatite (HA) were also incorporated in the polymer matrix to realize nanocomposite membranes. Scanning Electron Microscopy (SEM) showed improved interfacial adhesion with HA for functionalized polymers, and highlighted substantial differences in the porosity. A relationship between the developed porous structure of the membrane and the chemical nature of grafted groups was proposed. Compared to virgin PCL, hydrophilicity increases for functionalized PCL, while the addition of HA influences significantly the hydrophilic characteristics only in the case of virgin polymer. A significant increase of in vitro degradation rate was found for PCL-MAGMA based membranes, and at lower extent of PCL-DMAEA membranes. The novel materials were investigated regarding their potential as support for cell growth in bone repair using multipotent mesenchymal stromal cells (MSC) as a model. MSC plated onto the various membranes were analyzed in terms of adhesion, proliferation and osteogenic capacity that resulted to be related to chemical as well as porous structure. In particular, PCL-DMAEA and the relative nanocomposite membranes are the most promising in terms of cell-biomaterial interactions. - Graphical abstract: Functionalized PCL is used to realize nanocomposites with hydroxyapatite (HA) in the form of microporous membranes. The influence of different grafted groups on mechanical properties, in vitro degradation, porous membrane structure and interaction with mesenchymal stromal cells (MSC) is discussed. - Highlights: • Functionalized PCL shows faster in vitro degradation rate. • Functionalized PCL shows superior cell adhesion, proliferation and differentiation. • Nanocomposites based

Functionalized PCL/HA nanocomposites as microporous membranes for bone regeneration

International Nuclear Information System (INIS)

Basile, Maria Assunta; Gomez d'Ayala, Giovanna; Malinconico, Mario; Laurienzo, Paola; Coudane, Jean; Nottelet, Benjamin; Ragione, Fulvio Della; Oliva, Adriana

2015-01-01

In the present work, microporous membranes based on poly(ε-caprolactone) (PCL) and PCL functionalized with amine (PCL-DMAEA) or anhydride groups (PCL-MAGMA) were realized by solvent–non solvent phase inversion and proposed for use in Guided Tissue Regeneration (GTR). Nanowhiskers of hydroxyapatite (HA) were also incorporated in the polymer matrix to realize nanocomposite membranes. Scanning Electron Microscopy (SEM) showed improved interfacial adhesion with HA for functionalized polymers, and highlighted substantial differences in the porosity. A relationship between the developed porous structure of the membrane and the chemical nature of grafted groups was proposed. Compared to virgin PCL, hydrophilicity increases for functionalized PCL, while the addition of HA influences significantly the hydrophilic characteristics only in the case of virgin polymer. A significant increase of in vitro degradation rate was found for PCL-MAGMA based membranes, and at lower extent of PCL-DMAEA membranes. The novel materials were investigated regarding their potential as support for cell growth in bone repair using multipotent mesenchymal stromal cells (MSC) as a model. MSC plated onto the various membranes were analyzed in terms of adhesion, proliferation and osteogenic capacity that resulted to be related to chemical as well as porous structure. In particular, PCL-DMAEA and the relative nanocomposite membranes are the most promising in terms of cell-biomaterial interactions. - Graphical abstract: Functionalized PCL is used to realize nanocomposites with hydroxyapatite (HA) in the form of microporous membranes. The influence of different grafted groups on mechanical properties, in vitro degradation, porous membrane structure and interaction with mesenchymal stromal cells (MSC) is discussed. - Highlights: • Functionalized PCL shows faster in vitro degradation rate. • Functionalized PCL shows superior cell adhesion, proliferation and differentiation. • Nanocomposites based
Avoimen lähdekoodin HA-tietokannat

OpenAIRE

Vartio, Sami

2014-01-01

Insinöörityössä perehdyttiin avoimen lähdekoodin periaatteisiin ja lisenssimalleihin sekä huomioitiin liiketaloudelliset lähtökohdat. Tavoitteena oli tutustua järjestelmän saatavuuteen sekä klusteroinnin periaatteisiin. Järjestelmän saatavuus (HA) on tietojärjestelmien suunnittelussa käytettävä käytäntö, joka pyrkii siihen että järjestelmä on aina käyttäjän käytettävissä. Aluksi käsiteltiin perustietoja sekä teoriaa. Tutkimuksen edetessä paneuduttiin laajemmin yleisimpiin avoimen lähdeko...
Clinical significance of determination of serum HA, PCIII and ADA levels in patients with chronic hepatitis C

International Nuclear Information System (INIS)

Zhang Jing; Yu Fan; Zhao Changxin

2006-01-01

Objective: To explore the changes of serum HA, PCIII and ADA levels in patients with chronic hepatitis C. Methods: Serum HA, PCIII and ADA levels were detected with RIA in 42 cases with chronic hepatitis C and 35 controls. Results: The levels of serum HA, PC III and ADA in the patients were significantly higher than those in controls (P<0.01). Serum HA, PC m and ADA levels were positive correlated (r=0.6178, 0.6211, P<0.01). Conclusion: The results suggested the serum HA, PC m and ADA levels can reflect the degree of hepatic injury in the patients with cirrhosis, detection of serum HA, PC m and ADA levels is helpful in estimating the status of the disease. (authors)
Vaccine-induced anti-HA2 antibodies promote virus fusion and enhance influenza virus respiratory disease.

Science.gov (United States)

Khurana, Surender; Loving, Crystal L; Manischewitz, Jody; King, Lisa R; Gauger, Phillip C; Henningson, Jamie; Vincent, Amy L; Golding, Hana

2013-08-28

Vaccine-induced disease enhancement has been described in connection with several viral vaccines in animal models and in humans. We investigated a swine model to evaluate mismatched influenza vaccine-associated enhanced respiratory disease (VAERD) after pH1N1 infection. Vaccinating pigs with whole inactivated H1N2 (human-like) virus vaccine (WIV-H1N2) resulted in enhanced pneumonia and disease after pH1N1 infection. WIV-H1N2 immune sera contained high titers of cross-reactive anti-pH1N1 hemagglutinin (HA) antibodies that bound exclusively to the HA2 domain but not to the HA1 globular head. No hemagglutination inhibition titers against pH1N1 (challenge virus) were measured. Epitope mapping using phage display library identified the immunodominant epitope recognized by WIV-H1N2 immune sera as amino acids 32 to 77 of pH1N1-HA2 domain, close to the fusion peptide. These cross-reactive anti-HA2 antibodies enhanced pH1N1 infection of Madin-Darby canine kidney cells by promoting virus membrane fusion activity. The enhanced fusion activity correlated with lung pathology in pigs. This study suggests a role for fusion-enhancing anti-HA2 antibodies in VAERD, in the absence of receptor-blocking virus-neutralizing antibodies. These findings should be considered during the evaluation of universal influenza vaccines designed to elicit HA2 stem-targeting antibodies.
Isothermal Crystallization Kinetics of HDPE/HA Compounds Irradiated with Sterilization Doses of Gamma Rays

International Nuclear Information System (INIS)

Albano, C.

2006-01-01

The objective of this work was to study the isothermal crystallization of High Density Polyethylene/Hydroxyapatite nanocomposites, with 2 and 5 ppc of HA, irradiated with 25 kGy (sterilization dose) of γ-Ray from a 60 C o source, at a rate of 4.8 kGy/h in air and at room temperature. The selected crystallization temperatures were 118, 117, 116 and 115 degree. The crystallization kinetics was analyzed using the Avrami's model whose parameters were optimized using a non-linear regression technique. Regression results show that the Avrami exponent varies between 1.8 and 1.5, meaning that the spherulitic growth is mainly two dimensional. Values for specific crystallization constant 'k' were found to be higher for HDPE/HA compounds than for pure HDPE, clearly indicating the presence of an HA nucleation effect. It was also observed that values for the specific crystallization constant 'k' decreases with increasing temperatures, being this effect more noticeable for HDPE/HA compounds than for pure HDPE. Regarding to irradiated samples, their 'k' values were found to be lower than those for non irradiated samples, the difference getting more significant with decreasing crystallization temperature. Simulation of experimental data with the Avrami's model show a clear influence of the crystallization temperature, the HA content in the sample and the amount of applied radiation. It was also observed that the Avrami model correlates satisfactorily experimental data for not irradiated samples of pure HDPE and HDPE/HA compounds at the highest crystallization (T c ). However, as the crystallization temperature decreases, the values simulated with the Avrami model increasingly deviate from experimental data, specifically at the highest values of the relative crystallinity. This effect is even stronger on irradiated samples of HDPE and HDPE/HA compounds
Sorption of Ni(II) on GMZ bentonite: effects of pH, ionic strength, foreign ions, humic acid and temperature.

Science.gov (United States)

Yang, Shitong; Li, Jiaxing; Lu, Yi; Chen, Yixue; Wang, Xiangke

2009-09-01

Bentonite has been widely studied in nuclear waste management because of its special physicochemical properties. In this work, the sorption of Ni(II) from aqueous solution onto GMZ bentonite as a function of contact time, pH, ionic strength, foreign ions, humic acid (HA) and temperature was investigated under ambient conditions. The results indicated that the pseudo-second-order rate equation simulated the kinetic sorption process well. The sorption of Ni(II) on GMZ bentonite was strongly dependent on pH and on ionic strength. At low pH, the sorption of Ni(II) was dominated by outer-sphere surface complexation and ion exchange with Na(+)/H(+) on GMZ bentonite surfaces, whereas inner-sphere surface complexation was the main sorption mechanism at high pH. A positive effect of HA on Ni(II) sorption was found at pH8. The Langmuir, Freundlich, and D-R models were used to simulate the sorption isotherms of Ni(II) at three different temperatures: 303.15, 318.15 and 333.15K. The thermodynamic parameters (DeltaH(0), DeltaS(0) and DeltaG(0)) of Ni(II) sorption on GMZ bentonite at the three different temperatures were calculated from the temperature-dependent sorption isotherms. The results indicated that the sorption process of Ni(II) on GMZ bentonite was endothermic and spontaneous. Experimental results indicate that GMZ bentonite is a suitable sorbent for pre-concentration and solidification of Ni(II) from large volume solutions.
Sorption of Ni(II) on GMZ bentonite: Effects of pH, ionic strength, foreign ions, humic acid and temperature

International Nuclear Information System (INIS)

Yang Shitong; Li Jiaxing; Lu Yi; Chen Yixue; Wang Xiangke

2009-01-01

Bentonite has been widely studied in nuclear waste management because of its special physicochemical properties. In this work, the sorption of Ni(II) from aqueous solution onto GMZ bentonite as a function of contact time, pH, ionic strength, foreign ions, humic acid (HA) and temperature was investigated under ambient conditions. The results indicated that the pseudo-second-order rate equation simulated the kinetic sorption process well. The sorption of Ni(II) on GMZ bentonite was strongly dependent on pH and on ionic strength. At low pH, the sorption of Ni(II) was dominated by outer-sphere surface complexation and ion exchange with Na + /H + on GMZ bentonite surfaces, whereas inner-sphere surface complexation was the main sorption mechanism at high pH. A positive effect of HA on Ni(II) sorption was found at pH 8. The Langmuir, Freundlich, and D-R models were used to simulate the sorption isotherms of Ni(II) at three different temperatures: 303.15, 318.15 and 333.15 K. The thermodynamic parameters (ΔH 0 , ΔS 0 and ΔG 0 ) of Ni(II) sorption on GMZ bentonite at the three different temperatures were calculated from the temperature-dependent sorption isotherms. The results indicated that the sorption process of Ni(II) on GMZ bentonite was endothermic and spontaneous. Experimental results indicate that GMZ bentonite is a suitable sorbent for pre-concentration and solidification of Ni(II) from large volume solutions.
Porous HA-Al2O3 composite characterization using corn starch as a porogen agent

International Nuclear Information System (INIS)

Silva, L.A.J. da; Galdino, A.G.S.; Cardoso, G.B.C.; Zavaglia, C.A.C.

2011-01-01

The porous ceramics based on hydroxyapatite have great potential for application in bone grafts due to its chemical similarity with the mineral phase of bone tissue, but have poor biomechanical properties, which cause limitations in its applications. This work aims to analyze the structural characteristics of porous ceramics obtained by addition of hydroxyapatite (HA, sintered in the laboratory), Corn Starch (CS, commercial) and Al 2 O 3 (ALCOA), at different temperatures. Samples were made of dense HA (100% HA), porous (70% HA - 30% CS) and with addition of 2.5%, 5% and 7.5% Al 2 O 3 porous composition. The samples were sintered at 1250°C, 1300 deg C and 135 0°C and characterized by: XRF, XRD, SEM and density by the Archimedes method. Concludes It is the possibility of obtaining samples porous HA / Al 2 O 3 using starch as porogenic agent. The temperature and concentration of Al 2 O 3 most appropriate were: 1250°C and 7.5%. (author)
Study of the gamma irradiation effects on the PMMA/HA and PMMA/SW

Energy Technology Data Exchange (ETDEWEB)

Silva, P., E-mail: silva@ivic.v [Instituto Venezolano de Investigaciones Cientificas, Centro de Fisica, Carretera Panamericana Km. 11, Caracas 1020-A (Venezuela, Bolivarian Republic of); Albano, C. [Universidad Central de Venezuela, Facultad de Ingenieria (Venezuela, Bolivarian Republic of); Instituto Venezolano de Investigaciones Cientificas, Centro de Quimica (Venezuela, Bolivarian Republic of); Perera, R. [Departamento de Mecanica, Universidad Simon Bolivar, Caracas 1080-A (Venezuela, Bolivarian Republic of); Dominguez, N. [Instituto Venezolano de Investigaciones Cientificas, Centro de Quimica (Venezuela, Bolivarian Republic of)

2010-03-15

The behavior of the poly(methyl methacrylate) (PMMA) under the action of gamma radiation has been sufficiently studied. In this work, we present results from melt flow index (MFI), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and electron paramagnetic resonance (EPR) of PMMA composites with hydroxyapatite (HA) and seaweed residues (SW) irradiated with gamma rays at 1.08 kGy/h. Composites of PMMA/HA and PMMA/SW with 10%, 20% and 30% of the filler were prepared. The results show an increase in the MFI values with the integral dose of radiation, being consistent with chain-scission reactions. No EPR signal was observed in pure PMMA, while in the composites, the typical EPR signal of the PMMA radicals was observed, which increased with the amount of HA or SW. When comparing the relative intensities of the EPR signals for both types of composites, a slight increase in the concentration of free radicals generated in the sample with SW respect to that of PMMA/HA composite was obtained. A decay of the total free radical concentration was observed as time elapsed.
Does PEEK/HA Enhance Bone Formation Compared With PEEK in a Sheep Cervical Fusion Model?

Science.gov (United States)

Walsh, William R; Pelletier, Matthew H; Bertollo, Nicky; Christou, Chris; Tan, Chris

2016-11-01

Polyetheretherketone (PEEK) has a wide range of clinical applications but does not directly bond to bone. Bulk incorporation of osteoconductive materials including hydroxyapatite (HA) into the PEEK matrix is a potential solution to address the formation of a fibrous tissue layer between PEEK and bone and has not been tested. Using in vivo ovine animal models, we asked: (1) Does PEEK-HA improve cortical and cancellous bone ongrowth compared with PEEK? (2) Does PEEK-HA improve bone ongrowth and fusion outcome in a more challenging functional ovine cervical fusion model? The in vivo responses of PEEK-HA Enhanced and PEEK-OPTIMA ® Natural were evaluated for bone ongrowth in the form of dowels implanted in the cancellous and cortical bone of adult sheep and examined at 4 and 12 weeks as well as interbody cervical fusion at 6, 12, and 26 weeks. The bone-implant interface was evaluated with radiographic and histologic endpoints for a qualitative assessment of direct bone contact of an intervening fibrous tissue later. Gamma-irradiated cortical allograft cages were evaluated as well. Incorporating HA into the PEEK matrix resulted in more direct bone apposition as opposed to the fibrous tissue interface with PEEK alone in the bone ongrowth as well as interbody cervical fusions. No adverse reactions were found at the implant-bone interface for either material. Radiography and histology revealed resorption and fracture of the allograft devices in vivo. Incorporating HA into PEEK provides a more favorable environment than PEEK alone for bone ongrowth. Cervical fusion was improved with PEEK-HA compared with PEEK alone as well as allograft bone interbody devices. Improving the bone-implant interface with a PEEK device by incorporating HA may improve interbody fusion results and requires further clinical studies.
High Altitude Affects Nocturnal Non-linear Heart Rate Variability: PATCH-HA Study

Directory of Open Access Journals (Sweden)

Christopher J. Boos

2018-04-01

Full Text Available Background: High altitude (HA exposure can lead to changes in resting heart rate variability (HRV, which may be linked to acute mountain sickness (AMS development. Compared with traditional HRV measures, non-linear HRV appears to offer incremental and prognostic data, yet its utility and relationship to AMS have been barely examined at HA. This study sought to examine this relationship at terrestrial HA.Methods: Sixteen healthy British military servicemen were studied at baseline (800 m, first night and over eight consecutive nights, at a sleeping altitude of up to 3600 m. A disposable cardiac patch monitor was used, to record the nocturnal cardiac inter-beat interval data, over 1 h (0200–0300 h, for offline HRV assessment. Non-linear HRV measures included Sample entropy (SampEn, the short (α1, 4–12 beats and long-term (α2, 13–64 beats detrend fluctuation analysis slope and the correlation dimension (D2. The maximal rating of perceived exertion (RPE, during daily exercise, was assessed using the Borg 6–20 RPE scale.Results: All subjects completed the HA exposure. The average age of included subjects was 31.4 ± 8.1 years. HA led to a significant fall in SpO2 and increase in heart rate, LLS and RPE. There were no significant changes in the ECG-derived respiratory rate or in any of the time domain measures of HRV during sleep. The only notable changes in frequency domain measures of HRV were an increase in LF and fall in HFnu power at the highest altitude. Conversely, SampEn, SD1/SD2 and D2 all fell, whereas α1 and α2 increased (p < 0.05. RPE inversely correlated with SD1/SD2 (r = -0.31; p = 0.002, SampEn (r = -0.22; p = 0.03, HFnu (r = -0.27; p = 0.007 and positively correlated with LF (r = 0.24; p = 0.02, LF/HF (r = 0.24; p = 0.02, α1 (r = 0.32; p = 0.002 and α2 (r = 0.21; p = 0.04. AMS occurred in 7/16 subjects (43.8% and was very mild in 85.7% of cases. HRV failed to predict AMS.Conclusion: Non-linear HRV is more sensitive to the
Understanding the undelaying mechanism of HA-subtyping in the level of physic-chemical characteristics of protein.

Science.gov (United States)

Ebrahimi, Mansour; Aghagolzadeh, Parisa; Shamabadi, Narges; Tahmasebi, Ahmad; Alsharifi, Mohammed; Adelson, David L; Hemmatzadeh, Farhid; Ebrahimie, Esmaeil

2014-01-01

The evolution of the influenza A virus to increase its host range is a major concern worldwide. Molecular mechanisms of increasing host range are largely unknown. Influenza surface proteins play determining roles in reorganization of host-sialic acid receptors and host range. In an attempt to uncover the physic-chemical attributes which govern HA subtyping, we performed a large scale functional analysis of over 7000 sequences of 16 different HA subtypes. Large number (896) of physic-chemical protein characteristics were calculated for each HA sequence. Then, 10 different attribute weighting algorithms were used to find the key characteristics distinguishing HA subtypes. Furthermore, to discover machine leaning models which can predict HA subtypes, various Decision Tree, Support Vector Machine, Naïve Bayes, and Neural Network models were trained on calculated protein characteristics dataset as well as 10 trimmed datasets generated by attribute weighting algorithms. The prediction accuracies of the machine learning methods were evaluated by 10-fold cross validation. The results highlighted the frequency of Gln (selected by 80% of attribute weighting algorithms), percentage/frequency of Tyr, percentage of Cys, and frequencies of Try and Glu (selected by 70% of attribute weighting algorithms) as the key features that are associated with HA subtyping. Random Forest tree induction algorithm and RBF kernel function of SVM (scaled by grid search) showed high accuracy of 98% in clustering and predicting HA subtypes based on protein attributes. Decision tree models were successful in monitoring the short mutation/reassortment paths by which influenza virus can gain the key protein structure of another HA subtype and increase its host range in a short period of time with less energy consumption. Extracting and mining a large number of amino acid attributes of HA subtypes of influenza A virus through supervised algorithms represent a new avenue for understanding and
Understanding the undelaying mechanism of HA-subtyping in the level of physic-chemical characteristics of protein.

Directory of Open Access Journals (Sweden)

Mansour Ebrahimi

Full Text Available The evolution of the influenza A virus to increase its host range is a major concern worldwide. Molecular mechanisms of increasing host range are largely unknown. Influenza surface proteins play determining roles in reorganization of host-sialic acid receptors and host range. In an attempt to uncover the physic-chemical attributes which govern HA subtyping, we performed a large scale functional analysis of over 7000 sequences of 16 different HA subtypes. Large number (896 of physic-chemical protein characteristics were calculated for each HA sequence. Then, 10 different attribute weighting algorithms were used to find the key characteristics distinguishing HA subtypes. Furthermore, to discover machine leaning models which can predict HA subtypes, various Decision Tree, Support Vector Machine, Naïve Bayes, and Neural Network models were trained on calculated protein characteristics dataset as well as 10 trimmed datasets generated by attribute weighting algorithms. The prediction accuracies of the machine learning methods were evaluated by 10-fold cross validation. The results highlighted the frequency of Gln (selected by 80% of attribute weighting algorithms, percentage/frequency of Tyr, percentage of Cys, and frequencies of Try and Glu (selected by 70% of attribute weighting algorithms as the key features that are associated with HA subtyping. Random Forest tree induction algorithm and RBF kernel function of SVM (scaled by grid search showed high accuracy of 98% in clustering and predicting HA subtypes based on protein attributes. Decision tree models were successful in monitoring the short mutation/reassortment paths by which influenza virus can gain the key protein structure of another HA subtype and increase its host range in a short period of time with less energy consumption. Extracting and mining a large number of amino acid attributes of HA subtypes of influenza A virus through supervised algorithms represent a new avenue for
Consolidation de centres de recherche sur la reconstruction en Haïti ...

International Development Research Centre (IDRC) Digital Library (Canada)

Haïti doit encore relever des défis de taille en ce qui a trait à l'édification de l'État et à la démocratisation. Un groupe de pays latino-américains assume un rôle de premier plan au sein des forces de maintien de la paix coordonnées par la Mission des Nations Unies pour la stabilisation en Haïti (MINUSTAH). L'efficacité de ...
CRED Shallow CTD Profiles; Kaua'i, Main Hawaiian Islands; Cruise: HA1008, Data Date Range: 20101030-20101031 (NODC Accession 0039382).

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
CRED Shallow CTD Profiles; Lana'i, Main Hawaiian Islands; Cruise: HA1008, Data Date Range: 20101021-20101023 (NODC Accession 0039382).

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
CRED Shallow CTD Profiles; Tutuila, American Samoa; Cruise: HA1201_LEGII&III, Data Date Range: 20120401-20120406 (NODC Accession 0107470)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
Mechanical properties of titanium-hydroxyapatite (Ti-HA) composite coating on stainless steel prepared by thermal spraying

Science.gov (United States)

Rosmamuhamadani, R.; Azhar, N. H.; Talari, M. K.; Yahaya, Sabrina M.; Sulaiman, S.; Ismail, M. I. S.

2017-09-01

Addition of hydroxyapatite (HA) can enhance the bioactivity of the common metallic implant due to its similarity with natural bones and teeth. In this investigation, high velocity oxy-fuel (HVOFT) technique was used to deposit titanium-hydroxyapatite (Ti-HA) composite on stainless steel substrate plate with different percentage of HA for biomedical applications. The aim of this research is to investigate the mechanical properties of Ti-HA coating such as hardness, adhesion strength and wear behaviour. The hardness and strength was determined by using SHIMADZU-microhardness Vickers tester and PosiTest AT portable adhesion tester respectively. The wear test was performed by using pin-on-disk equipment and field emission scanning electron microscope (FESEM) used to determine the extent of surface damage. From the results obtained, mechanical properties such as hardness and adhesion strength of titanium (Ti) coating decreased with the increased of HA contents. Meanwhile, the coefficient of friction of Ti-10% HA coating shows the highest value compare to others as three-body abrasion had occurred during the test.
Magneto-optical and catalytic properties of Fe{sub 3}O{sub 4}@HA@Ag magnetic nanocomposite

Energy Technology Data Exchange (ETDEWEB)

Amir, Md., E-mail: mdamir01031001@gmail.com [Department of Chemistry, Fatih University, 34500 B.Çekmece-İstanbul, Turkey (Turkey); Bio & Nanotechnology Engineering, Faculty of Engineering, Istanbul University, 34452 Beyazıt/Fatih-İstanbul (Turkey); Güner, S. [Department of Physics, Fatih University, 34500 B.Çekmece-İstanbul, Turkey (Turkey); Yıldız, A. [Department of Textile Engineering, Namık Kemal University, 59860 Çorlu-Tekirdağ, Turkey (Turkey); Baykal, A. [Department of Chemistry, Fatih University, 34500 B.Çekmece-İstanbul, Turkey (Turkey)

2017-01-01

Fe{sub 3}O{sub 4}@HA@Ag magnetic nanocomposites (MNCs) were successfully synthesized by the simple reflux method for the removal of azo dyes from the industrial aqueous media. Fe{sub 3}O{sub 4}@HA@AgMNCs exhibited high catalytic activity to reduce MB within 20 min from the waste water. The obtained materials were characterized by the means of different techniques. Powder X-ray diffraction (XRD) analysis confirmed the single-phase of Fe{sub 3}O{sub 4} spinel structure. SEM and TEM analysis indicated that Fe{sub 3}O{sub 4}@HA@AgMNCs were nanoparticles like structure with small agglomeration. TG result showed that the products contained 9% of HA. The characteristic peaks of HA at 1601 cm{sup −1} and 1703 cm{sup −1} was observed by the means of FT-IR spectra of Fe{sub 3}O{sub 4}@HA@AgMNCs. The hysteresis (σ–H) curves revealed Fe{sub 3}O{sub 4}@HA@Ag MNCs exhibit a typical superparamagnetic characteristic with a saturation magnetization of 59.11 emu/g and measured magnetic moment is 2.45 µ{sub B}. The average magnetic particle dimension (D{sub mag}) is 13.25 nm. In accordance, the average crystallite and particle dimensions were obtained as 11.50 nm and 13.10 nm from XRD and TEM measurements, respectively. Magnetocrystalline anisotropy was offered as uniaxial and calculated effective anisotropy constant (K{sub eff}) is 2.96×10{sup 5} Erg/g. The blocking temperature was estimated as 522 K. The size-dependent saturation magnetization suggests the existence of a magnetically dead layer as 0.793 nm for Fe{sub 3}O{sub 4}@HA@Ag MNCs. The UV–vis diffuse reflectance spectroscopy (DRS) and Kubelka–Munk theory were applied to determine the optical properties of powder samples. The direct optical energy band gap (E{sub g}) values were estimated from Tauc plots between 1.62 eV and 2.12 eV. - Graphical abstract: An illustration for the fabrication of Fe{sub 3}O{sub 4}@HA@Ag MNCs. - Highlights: • The reduction of azo dye such Methylene Blue was instantly completed with
Antibiotic-containing hyaluronic acid gel as an antibacterial carrier: Usefulness of sponge and film-formed HA gel in deep infection.

Science.gov (United States)

Matsuno, Hiroaki; Yudoh, Kazuo; Hashimoto, Masamichi; Himeda, Yasukazu; Miyoshi, Teruzo; Yoshida, Kaoru; Kano, Syogo

2006-03-01

We have developed a novel bioabsorbable antibacterial carrier using hyaluronic acid (HA) gel for prevention and treatment of orthopedic infections. In this study, we investigated the in vivo antibacterial effects of two forms of this new material, an HA gel sponge and an HA gel film. A titanium cylinder was inserted into the intramedullary cavity of each rabbit femur, along with an HA gel sponge or HA gel film containing antibiotics. The HA gel sponge contained gentamycin, vancomycin, tobramycin, or minomycin. The HA gel film contained gentamycin or vancomycin. After 0, 7, and 14 days, the rabbit bone marrow was collected, and the antibacterial activity of the HA gel was determined by agar diffusion test. As a control, we used Septocoll, a commercially available antibacterial carrier. Both the HA gel sponge and HA gel film exhibited antibacterial activity. The present results indicate that HA gel containing antibiotics is a clinically useful bioabsorbable antibacterial carrier. Copyright 2006 Orthopaedic Research Society.

Bone Tissue Response to Nano-HA-ZrO2 Compound Bioceramics%纳米增韧HA-ZrO2生物陶瓷与骨缺损断面的结合性能观察

Institute of Scientific and Technical Information of China (English)

孟志兵; 唐月军; 郭军; 吕春堂; 周中华

2013-01-01

目的:观察纳米增韧HA-ZrO2生物陶瓷植入体与骨断面的结合情况.方法:12只健康成年新西兰大白兔,双侧下颌骨制备洞穿性骨缺损,一侧植入纳米增韧HA-ZrO2生物陶瓷,对侧植入HA(羟基磷灰石)材料,4、8、12周时取材,进行X线摄片观察、材料顶出实验、硬组织切片,比较观察2组骨与材料界面结合情况.结果:X线片和硬组织切片观察结果显示4、8周时HA-ZrO2、HA和骨断面结合不良,12周时发现两种材料均和骨断面结合良好,少部分区域有纤维组织包绕.4、8、12周纳米增韧HA-ZrO2组抗剪切强度分别为(2.36±1.04)、(10.65±1.48)和(30.95±3.67)MPa,不同时间段的组内差异有统计学意义;4、8、12周HA组抗剪切强度分别为(2.27±1.12)、(12.33±1.57)和(31.05±3.44)MPa,不同时间段组内比较差异有统计学意义(P＜0.01);但同时间段的结果组间比较,差异无统计学意义(P＞0.05).结论:初步认为纳米增韧HA-ZrO2生物陶瓷和骨断面,可以取得与HA类似的较好结合,但是如何在尽量短的时间内使其与骨断面完全结合,还需要进一步的研究.%Objective:The purpose of the present study was to observe the combination of the interface between nanoHA-ZrO2 compound bioceramics and bone tissue.Methods:12 healthy adult New Zealand rabbits were used in the study.Nano-HA-ZrO2 compound bioceramics were placed in one side of the mandible.On the other side,HA were placed as control.Animals were sacrificed at 4、8、12 weeks after operation,histological sections and the interface shear strength were evaluated.X-ray examination were also performed to observe the interface between the bone,nano-HA-ZrO2,and HA bioceramics materials.Results:Hard tissue section and X-rays examination showed a good interface osseointegration appeared at 12 weeks in nano-HA-ZrO2 ceramic group,as well as in HA group.Fibrous tissue manifested in some areas of the interface.Incomplete osseointegration were shown in the
Aerosol extinction profiles at 525 nm and 1020 nm derived from ACE imager data: comparisons with GOMOS, SAGE II, SAGE III, POAM III, and OSIRIS

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F. Vanhellemont

2008-04-01

Full Text Available The Canadian ACE (Atmospheric Chemistry Experiment mission is dedicated to the retrieval of a large number of atmospheric trace gas species using the solar occultation technique in the infrared and UV/visible spectral domain. However, two additional solar disk imagers (at 525 nm and 1020 nm were added for a number of reasons, including the retrieval of aerosol and cloud products. In this paper, we present first comparison results for these imager aerosol/cloud optical extinction coefficient profiles, with the ones derived from measurements performed by 3 solar occultation instruments (SAGE II, SAGE III, POAM III, one stellar occultation instrument (GOMOS and one limb sounder (OSIRIS. The results indicate that the ACE imager profiles are of good quality in the upper troposphere/lower stratosphere, although the aerosol extinction for the visible channel at 525 nm contains a significant negative bias at higher altitudes, while the relative differences indicate that ACE profiles are almost always too high at 1020 nm. Both problems are probably related to ACE imager instrumental issues.
Higher Education and Development in South-East Asia. Volume II, Country Profiles.

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Hayden, Howard; And Others

This document, the second of three volumes concerned with the role of institutions of higher education in the development of countries in South-East Asia, presents country profiles for Burma, Thailand, Laos, Cambodia, Viet-Nam, Malaysia, Singapore, Indonesia, and the Philippines. The profile emphasizes background, higher education, educational…
Improving natural resource management in Viet Nam's Hong Ha ...

International Development Research Centre (IDRC) Digital Library (Canada)

2011-07-15

Jul 15, 2011 ... Improving natural resource management in Viet Nam's Hong Ha commune ... In this work, the research team helped local farmers improve their land ... with a home garden economy, limit forest destruction, diversify crop production, ... low female participation rates in decision making; limited education; rapid ...
40 CFR 141.709 - Developing the disinfection profile and benchmark.

Science.gov (United States)

2010-07-01

... Cryptosporidium Disinfection Profiling and Benchmarking Requirements § 141.709 Developing the disinfection profile...) of the water before or at the first customer and prior to each additional point of disinfectant...) before or at the first customer during peak hourly flow. (ii) Determine successive CTcalc/CT99.9 values...
Jom ha-Šo'a...počátky jednoho svátku

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Zbyněk Tarant

2011-12-01

Full Text Available If one looks at the calendar, one will find there 'International Holocaust Rememberance Day' on 27th of January. This day was set by the UN General Assembly in 2005 and it refers to the anniversary of Auschwitz liberation. However, if one looks at the Israeli calendar, or any of the Jewish Luakh haShannah's, one will find there another day of mourning that is actually far older. The Yom HaShoah was set according to the Hebrew calendar and refers not to the liberation of Auschwitz, but to the Warsaw ghetto uprising. Today, this day of mourning is an interesting relict of the pioneer, upbuilding era that preferred the stories of heroes to those of victims. This article attempts to map the very beginning of the Yom HaShoah commemoration, by using Hebrew sources that were never published before in the Czech language. The story of Yom HaShoah in the context of the young state of Israel in the 1950s is an interesting case-study on the issue of how the contemporary political and public discourse affects the collective memory of future generations.
DEMENCIA FRONTOTEMPORAL, CÓMO HA RESURGIDO SU DIAGNÓSTICO

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Patricia Lillo Z, MD, PhD

2016-05-01

Full Text Available Desde la descripción inicial efectuada por Pick hace más de un siglo atrás, el interés por las demencias frontotemporales ha tenido un gran crecimiento. Gracias a los avances de las neuroimágenes y el desarrollo de biomarcadores, el progreso en este campo ha generado nuevos conocimientos en torno a la categorización clínica, correlatos neuronales de las funciones cerebrales superiores y mecanismos de neurodegeneración. Paradójicamente, estos avances han traído consigo cierta controversia y desafíos, los cuales han motivado la creación de nuevos criterios diagnósticos. Esta breve revisión muestra la evolución histórica de estas condiciones y describe los criterios diagnósticos actuales para la variante conductual y las afasias progresivas primarias, que son las dos formas clínicas de las demencias frontotemporales.
Functional study of the novel multidrug resistance gene HA117 and its comparison to multidrug resistance gene 1

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Chen Tingfu

2010-07-01

Full Text Available Abstract Background The novel gene HA117 is a multidrug resistance (MDR gene expressed by all-trans retinoic acid-resistant HL-60 cells. In the present study, we compared the multidrug resistance of the HA117 with that of the classical multidrug resistance gene 1 (MDR1 in breast cancer cell line 4T1. Methods Transduction of the breast cancer cell line 4T1 with adenoviral vectors encoding the HA117 gene and the green fluorescence protein gene (GFP (Ad-GFP-HA117, the MDR1 and GFP (Ad-GFP-MDR1 or GFP (Ad-GFP was respectively carried out. The transduction efficiency and the multiplicity of infection (MOI were detected by fluorescence microscope and flow cytometry. The transcription of HA117 gene and MDR1 gene were detected by reverse transcription polymerase chain reaction (RT-PCR. Western blotting analysis was used to detect the expression of P-glycoprotein (P-gp but the expression of HA117 could not be analyzed as it is a novel gene and its antibody has not yet been synthesized. The drug-excretion activity of HA117 and MDR1 were determined by daunorubicin (DNR efflux assay. The drug sensitivities of 4T1/HA117 and 4T1/MDR1 to chemotherapeutic agents were detected by Methyl-Thiazolyl-Tetrazolium (MTT assay. Results The transducted efficiency of Ad-GFP-HA117 and Ad-GFP-MDR1 were 75%-80% when MOI was equal to 50. The transduction of Ad-GFP-HA117 and Ad-GFP-MDR1 could increase the expression of HA117 and MDR1. The drug resistance index to Adriamycin (ADM, vincristine (VCR, paclitaxel (Taxol and bleomycin (BLM increased to19.8050, 9.0663, 9.7245, 3.5650 respectively for 4T1/HA117 and 24.2236, 11.0480, 11.3741, 0.9630 respectively for 4T1/MDR1 as compared to the control cells. There were no significant differences in drug sensitivity between 4T1/HA117 and 4T1/MDR1 for the P-gp substrates (ADM, VCR and Taxol (P Conclusions These results confirm that HA117 is a strong MDR gene in both HL-60 and 4T1 cells. Furthermore, our results indicate that the MDR
Serum expression of HA and LN in lewis rat models of autoimmune myocarditis

International Nuclear Information System (INIS)

Han Li'na; Li Tieling; Zhang Yajing; Yang Tingshu; Ding Yu; Guo Shuli; Zhao Xiaoning

2011-01-01

Objective: To study the clinical significance of dynamic changes of serum expressions of hyaluronic acid (HA) and laminin (LN) in Lewis rat models of experimental autoimmune myocarditis (EAM). Methods: Fifty Lewis rat models of experimental autoimmune myocarditis (EAM) were established with injection of recombinant cardiac C protein with complete freund adjuvant into two foot-pads plus intraperitoneal injection of pertussis toxin. At 1w, 2w, 4w, 6w and 8w, 10 models were sacrificed each time;cardiac tissues were examined with HE stain for myocardial inflammatory score and examined with picrosirius red stain for myocardial fibrosis score, also, serum HA and LN expressions were determined with RIA. These examinations were performed in 10 undisturbed animals as controls. Results: The myocardial inflammatory scores of the models at 1w were about the same as those in the controls, but the scores rapidly increased from 2w on to 4w then fell gradually. The myocardial fibrosis scores of the models at 1wk were also not much different from those in controls. The fibrosis scores increased rapidly at 4w and maintained at high level up to 8w. The changes of serum expressions of HA and LN roughly paralleled those of myocardial fibrosis scores i. e. rapidly increased at 4w up to 8w. Conclusion: Serum expressions of HA and LN could faithfully reflect the degree of myocardial fibrosis in rat models of EAM. HA and LN were useful markers of myocardial fibrois and were of prognostic importance. (authors)
3D-printed scaffolds based on PLA/HA nanocomposites for trabecular bone reconstruction

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Niaza, K. V.; Senatov, F. S.; Kaloshkin, S. D.; Maksimkin, A. V.; Chukov, D. I.

2016-08-01

In the present work porous PLA scaffolds filled with micro- and nano- HA were studied. Both composites with micro- and nano-HA were obtained by extrusion in the same conditions. Scaffolds were obtained by 3D-printing by fused filament fabrication method. Structure of porous scaffolds was pre-modeled by computer software. Compression and three - point flexural tests were used to study mechanical properties of the scaffolds.
Role of induced glutathione-S-transferase from Helicoverpa armigera (Lepidoptera: Noctuidae) HaGST-8 in detoxification of pesticides.

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Labade, Chaitali P; Jadhav, Abhilash R; Ahire, Mehul; Zinjarde, Smita S; Tamhane, Vaijayanti A

2018-01-01

The present study deals with glutathione-S-transferase (GST) based detoxification of pesticides in Helicoverpa armigera and its potential application in eliminating pesticides from the environment. Dietary exposure of a pesticide mixture (organophosphates - chlorpyrifos and dichlorvos, pyrethroid - cypermethrin; 2-15ppm each) to H. armigera larvae resulted in a dose dependant up-regulation of GST activity and gene expression. A variant GST from H. armigera (HaGST-8) was isolated from larvae fed with 10ppm pesticide mixture and it was recombinantly expressed in yeast (Pichia pastoris HaGST-8). HaGST-8 had a molecular mass of 29kDa and was most active at pH 9 at 30°C. GC-MS and LC-HRMS analysis validated that HaGST-8 was effective in eliminating organophosphate type of pesticides and partially reduced the cypermethrin content (53%) from aqueous solutions. Unlike the untransformed yeast, P. pastoris HaGST-8 grew efficiently in media supplemented with pesticide mixtures (200 and 400ppm each pesticide) signifying the detoxification ability of HaGST-8. The amino acid sequence of HaGST-8 and the already reported sequence of HaGST-7 had just 2 mismatches. The studies on molecular interaction strengths revealed that HaGST-8 had stronger binding affinities with organophosphate, pyrethroid, organochloride, carbamate and neonicotinoid type of pesticides. The abilities of recombinant HaGST-8 to eliminate pesticides and P. pastoris HaGST-8 to grow profusely in the presence of high level of pesticide content can be applied for removal of such residues from food, water resources and bioremediation. Copyright © 2017 Elsevier Inc. All rights reserved.
Bioinformatics Approach Based Research of Profile Protein Carbonic Anhydrase II Analysis as a Potential Candidate Cause Autism for The Variation of Learning Subjects Biotechnology

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Dian Eka A. F. Ningrum

2017-03-01

Full Text Available This study aims to determine the needs of learning variations on Biotechnology courses using bioinformatics approaches. One example of applied use of bioinformatics in biotechnology course is the analysis of protein profiles carbonic anhydrase II as a potential cause of autism candidate. This research is a qualitative descriptive study consisted of two phases. The first phase of the data obtained from observations of learning, student questionnaires, and questionnaires lecturer. Results from the first phase, namely the need for variations learning in Biotechnology course using bioinformatics. Collecting data on the second stage uses three webserver to predict the target protein and scientific articles. Visualization of proteins using PyMOL software. 3 based webserver which is used, the candidate of target proteins associated with autism is carbonic anhydrase II. The survey results revealed that the protein carbonic anhydrase II as a potential candidate for the cause of autism classified metaloenzim are able to bind with heavy metals. The content of heavy metals in autistic patients high that affect metabolism. This prediction of protein candidate cause autism is applied use to solve the problem in society, so that can achieve the learning outcome in biotechnology course.
Influence of Peripheral Artery Disease and Statin Therapy on Apolipoprotein Profiles

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Andrew W. Gardner

2013-01-01

Full Text Available Apolipoprotein B is a stronger predictor of myocardial infarction than LDL cholesterol, and it is inversely related to physical activity and modifiable with exercise training. As such, apolipoprotein measures may be of particular relevance for subjects with PAD and claudication. We compared plasma apolipoprotein profiles in 29 subjects with peripheral artery disease (PAD and intermittent claudication and in 39 control subjects. Furthermore, we compared the plasma apolipoprotein profiles of subjects with PAD either treated (n=17 or untreated (n=12 with statin medications. For the apolipoprotein subparticle analyses, subjects with PAD had higher age-adjusted Lp-B:C (P<0.05 and lower values of Lp-A-I:A-II (P<0.05 than controls. The PAD group taking statins had lower age-adjusted values for apoB (P<0.05, Lp-A-II:B:C:D:E (P<0.05, Lp-B:E + Lp-B:C:E (P<0.05, Lp-B:C (P<0.05, and Lp-A-I (P<0.05 than the untreated PAD group. Subjects with PAD have impaired apolipoprotein profiles than controls, characterized by Lp-B:C and Lp-A-I:A-II. Furthermore, subjects with PAD on statin medications have a more favorable risk profile, particularly noted in multiple apolipoprotein subparticles. The efficacy of statin therapy to improve cardiovascular risk appears more evident in the apolipoprotein sub-particle profile than in the more traditional lipid profile of subjects with PAD and claudication. This trial is registered with ClinicalTrials.gov NCT00618670.
Bør leger ha reservasjonsrett ved assistert befruktning?

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Morten Magelssen

2011-10-01

Full Text Available Omfanget av helsepersonells reservasjonsrett har nylig vært gjenstand for debatt i Norge. Vi spør om leger bør ha reservasjonsrett ved utførelse og henvisning til assistert befruktning, og drøfter argumenter for og imot ved hjelp av et rammeverk med sju kriterier for vurdering av reservasjon. Reservasjonsrettens grunnleggende dilemma er hvordan to viktige hensyn, henholdsvis pasientens rett til behandling og hensynet til helsepersonellets moralske integritet, best kan ivaretas. Det argumenteres for at leger bør ha rett til å reservere seg mot å utføre, assistere ved og henvise til assistert befruktning generelt hvis begrunnelsen er hensynet til befruktede eggs moralske verdi. Videre finner vi at leger også kan ha en moralsk rett til reservasjon mot å utføre, assistere ved og henvise til assistert befruktning for likekjønnede, men da på nærmere spesifiserte vilkår.Nøkkelord: reservasjonsrett, assistert befruktning, samvittighet, moralsk integritetEnglish summary: Should physicians have the right to conscientiously object to assisted reproduction?The extent of the healthcare worker's right to conscientious objection has recently been debated in Norway. This article asks whether physicians should have a right to conscientious objection to the performance of, and referral for, assisted reproduction, and discusses arguments for and against the same, utilizing a framework of seven criteria for the evaluation of conscientious objection. The fundamental dilemma of conscientious objection is how two important considerations can be reconciled: the patient's right to treatment, and the protection of the healthcare worker's moral integrity. It is argued that physicians should have the right to object to performing, assisting with, and referring for assisted reproduction generally when the objection is grounded in the moral value of the embryo. Furthermore, physicians may also have a moral right to object to performing, assisting with, and
[Study on biocompatibility of hydroxyapatite/high density polyethylene (HA/HDPE) nano-composites artificial ossicle].

Science.gov (United States)

Wang, Guohui; Zhu, Shaihong; Tan, Guolin; Zhou, Kechao; Huang, Suping; Zhao, Yanzhong; Li, Zhiyou; Huang, Boyun

2008-06-01

This study was aimed to evaluate the biocompatibility of Hydroxyapatite/High density polyethylene (HA/ HDPE) nano-composites artificial ossicle. The percentage of S-period cells were detected by flow cytometry after L929 cells being incubated with extraction of the HA/HDPE nano-composites; the titanium materials for clinical application served as the contrast. In addition, both materials were implanted in animals and the histopathological evaluations were conducted. There were no statistically significant differences between the two groups (P >0.05). The results demonstrated that the HA/HDPE nano-composite artificial ossicle made by our laboratory is of a good biocompatibility and clinical application outlook.
Enhanced expression of IL-8 in normal human keratinocytes and human keratinocyte cell line HaCaT in vitro after stimulation with contact sensitizers, tolerogens and irritants.

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Mohamadzadeh, M; Müller, M; Hultsch, T; Enk, A; Saloga, J; Knop, J

1994-12-01

To investigate the interleukin-8 production of keratinocytes after stimulation in vitro we have used various agents: (i) contact sensitizer (2,4-dinitrofluorobenzene, 3-n-pentadecylcatechol); (ii) tolerogen (5-methyl-3-n-pentadecylcatechol); (iii) irritant (sodium lauryl sulfate). Interleukin-8 gene expression was assessed by northern blot hybridization of the total cytoplasmic RNA extracted from subconfluent normal human keratinocyte cultures and the keratinocyte cell line HaCaT using a radiolabeled DNA probe specific for human interleukin-8. Interleukin-8 gene expression was markedly increased upon in vitro stimulation after 1-6 h with contact sensitizers, tolerogen and the irritant. In contrast, interleukin-8 production was not detectable in unstimulated normal human keratinocytes or the HaCaT keratinocyte cell line. These results suggest that the induction and production of interleukin-8 is a response to nonspecific stimuli and may play a critical role in the early response to immunogenic or inflammatory signals in man.
HaNDL Syndrome Presenting During Pregnancy: A Case Report and Review of the Literature

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Yüksel Kaplan

2014-09-01

Full Text Available Headache associated with neurological deficits and cerebrospinal fluid lymphocytosis (HaNDL is a self-limited syndrome characterized by sudden-onset headache with a temporary neurological deficit and cerebrospinal fluid (CSF lymphocytosis. We aimed to disscus a case of HaNDL syndrome presenting during pregnancy with relevant literature. A 20-year-old female presented with a 5-day history of severe, bilateral throbbing headache accompanied by nausea, vomiting, and phonophobia. Approximately 2 days after the pain developed, she became acutely confused for less than 90 minutes. 2 days after this episode, she experienced again confusional state and left hemiparesis. There were no symptoms consistent with meningoencephalitis. She was pregnant and at 11 weeks gestation. A neurologic examination showed confusional state, bilateral papilledema, and mild left hemiparesis. The neuroradiological examination was normal. The cerebrospinal fluid revealed lymphocytic pleocytosis, mildly elevated protein, and increased opening pressure. She recovered completely after 8 days. The precise etiology of HaNDL is unknown, although an inflammatory or infectious origin and autoimmune factors have been proposed. Moreover, the risk factors and medical conditions associated with HaNDL are unknown. It is obviously difficult to determine whether the pregnancy was coincidental or associated in this case. We believe that comprehensive studies are needed to clarify the risk factors and medical conditions associated with HaNDL
Neural stem cell proliferation and differentiation in the conductive PEDOT-HA/Cs/Gel scaffold for neural tissue engineering.

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Wang, Shuping; Guan, Shui; Xu, Jianqiang; Li, Wenfang; Ge, Dan; Sun, Changkai; Liu, Tianqing; Ma, Xuehu

2017-09-26

Engineering scaffolds with excellent electro-activity is increasingly important in tissue engineering and regenerative medicine. Herein, conductive poly(3,4-ethylenedioxythiophene) doped with hyaluronic acid (PEDOT-HA) nanoparticles were firstly synthesized via chemical oxidant polymerization. A three-dimensional (3D) PEDOT-HA/Cs/Gel scaffold was then developed by introducing PEDOT-HA nanoparticles into a chitosan/gelatin (Cs/Gel) matrix. HA, as a bridge, not only was used as a dopant, but also combined PEDOT into the Cs/Gel via chemical crosslinking. The PEDOT-HA/Cs/Gel scaffold was used as a conductive substrate for neural stem cell (NSC) culture in vitro. The results demonstrated that the PEDOT-HA/Cs/Gel scaffold had excellent biocompatibility for NSC proliferation and differentiation. 3D confocal fluorescence images showed cells attached on the channel surface of Cs/Gel and PEDOT-HA/Cs/Gel scaffolds with a normal neuronal morphology. Compared to the Cs/Gel scaffold, the PEDOT-HA/Cs/Gel scaffold not only promoted NSC proliferation with up-regulated expression of Ki67, but also enhanced NSC differentiation into neurons and astrocytes with up-regulated expression of β tubulin-III and GFAP, respectively. It is expected that this electro-active and bio-active PEDOT-HA/Cs/Gel scaffold will be used as a conductive platform to regulate NSC behavior for neural tissue engineering.
Perturbative Heat Transport Experiments on TJ-II

International Nuclear Information System (INIS)

Eguilor, S.; Castejon, F.; Luna, E. de la; Cappa, A.; Likin, K.; Fernandez, A.; Tj-II, T.

2002-01-01

Heat wave experiments are performed on TJ-II stellarator plasmas to estimate both heat diffusivity and power deposition profiles. High frequency ECRH modulation experiments are used to obtain the power deposition profiles, which is observed to be wider and duller than estimated by tracing techniques. The causes of this difference are discussed in the paper. Fourier analysis techniques are used to estimate the heat diffusivity in low frequency ECRH modulation experiments. This include the power deposition profile as a new ingredient. ECHR switch on/off experiments are exploited to obtain power deposition and heat diffusivities profile. Those quantities are compared with the obtained by modulation experiments and transport analysis, showing a good agreement. (Author) 18 refs
Perturbative Heat Transport Experiments on TJ-II

Energy Technology Data Exchange (ETDEWEB)

Eguilor, S.; Castejon, F.; Luna, E. de la; Cappa, A.; Likin, K.; Fernandez, A.; Tj-II, T.

2002-07-01

Heat wave experiments are performed on TJ-II stellarator plasmas to estimate both heat diffusivity and power deposition profiles. High frequency ECRH modulation experiments are used to obtain the power deposition profiles, which is observed to be wider and duller than estimated by tracing techniques. The causes of this difference are discussed in the paper. Fourier analysis techniques are used to estimate the heat diffusivity in low frequency ECRH modulation experiments. This include the power deposition profile as a new ingredient. ECHR switch on/off experiments are exploited to obtain power deposition and heat diffusivities profile. Those quantities are compared with the obtained by modulation experiments and transport analysis, showing a good agreement. (Author) 18 refs.

Belgia rahvuspüha puhul kuuleb Eestis kammermuusikat / Maarja Pehk

Index Scriptorium Estoniae

Pehk, Maarja

2007-01-01

21. juulil on Belgia Kuningriigil rahvuspüha. Suursaadik Pierre Clement Dubuisson tutvustab sel puhul belgia kultuuri kolme kontserdi kaudu. Sel aastal tutvustab saatkond Belgia kultuuri ka koostöös Pimedate Ööde filmifestivaliga
3D-printed scaffolds based on PLA/HA nanocomposites for trabecular bone reconstruction

International Nuclear Information System (INIS)

Niaza, K V; Senatov, F S; Kaloshkin, S D; Maksimkin, A V; Chukov, D I

2016-01-01

In the present work porous PLA scaffolds filled with micro- and nano- HA were studied. Both composites with micro- and nano-HA were obtained by extrusion in the same conditions. Scaffolds were obtained by 3D-printing by fused filament fabrication method. Structure of porous scaffolds was pre-modeled by computer software. Compression and three - point flexural tests were used to study mechanical properties of the scaffolds. (paper)
Study on the correlationship between serum IGF-I and HA, PIIIP levels in patients with chronic hepatitis C

International Nuclear Information System (INIS)

Li Yong; Sun Yefu; Xia Aiping

2008-01-01

Objective: To study the inter-relationship between serum IGF-I and HA, PIIIP levels in patients with chronic hepatitis C. Methods: Serum IGF-I, HA and PIIIP levels were determined with RIA in 39 patients with chronic hepatitis C and 35 controls. Results: Serum IGF-I, HA and PIIIP levels in the patients were significantly higher than those in controls (P<0.01). The serum IGF-I levels and HA, PIIIP levels were mutually positively correlated (r=0.8018, 0.7126, P<0.01). Conclusion: Serum IGF-I, HA and PIIIP were useful diagnostic markers in patients with chronic hepatitis C. (authors)
Near infrared spectroscopy combined with multivariate analysis for monitoring the ethanol precipitation process of fraction I + II + III supernatant in human albumin separation

Science.gov (United States)

Li, Can; Wang, Fei; Zang, Lixuan; Zang, Hengchang; Alcalà, Manel; Nie, Lei; Wang, Mingyu; Li, Lian

2017-03-01

Nowadays, as a powerful process analytical tool, near infrared spectroscopy (NIRS) has been widely applied in process monitoring. In present work, NIRS combined with multivariate analysis was used to monitor the ethanol precipitation process of fraction I + II + III (FI + II + III) supernatant in human albumin (HA) separation to achieve qualitative and quantitative monitoring at the same time and assure the product's quality. First, a qualitative model was established by using principal component analysis (PCA) with 6 of 8 normal batches samples, and evaluated by the remaining 2 normal batches and 3 abnormal batches. The results showed that the first principal component (PC1) score chart could be successfully used for fault detection and diagnosis. Then, two quantitative models were built with 6 of 8 normal batches to determine the content of the total protein (TP) and HA separately by using partial least squares regression (PLS-R) strategy, and the models were validated by 2 remaining normal batches. The determination coefficient of validation (Rp2), root mean square error of cross validation (RMSECV), root mean square error of prediction (RMSEP) and ratio of performance deviation (RPD) were 0.975, 0.501 g/L, 0.465 g/L and 5.57 for TP, and 0.969, 0.530 g/L, 0.341 g/L and 5.47 for HA, respectively. The results showed that the established models could give a rapid and accurate measurement of the content of TP and HA. The results of this study indicated that NIRS is an effective tool and could be successfully used for qualitative and quantitative monitoring the ethanol precipitation process of FI + II + III supernatant simultaneously. This research has significant reference value for assuring the quality and improving the recovery ratio of HA in industrialization scale by using NIRS.
Near infrared spectroscopy combined with multivariate analysis for monitoring the ethanol precipitation process of fraction I+II+III supernatant in human albumin separation.

Science.gov (United States)

Li, Can; Wang, Fei; Zang, Lixuan; Zang, Hengchang; Alcalà, Manel; Nie, Lei; Wang, Mingyu; Li, Lian

2017-03-15

Nowadays, as a powerful process analytical tool, near infrared spectroscopy (NIRS) has been widely applied in process monitoring. In present work, NIRS combined with multivariate analysis was used to monitor the ethanol precipitation process of fraction I+II+III (FI+II+III) supernatant in human albumin (HA) separation to achieve qualitative and quantitative monitoring at the same time and assure the product's quality. First, a qualitative model was established by using principal component analysis (PCA) with 6 of 8 normal batches samples, and evaluated by the remaining 2 normal batches and 3 abnormal batches. The results showed that the first principal component (PC1) score chart could be successfully used for fault detection and diagnosis. Then, two quantitative models were built with 6 of 8 normal batches to determine the content of the total protein (TP) and HA separately by using partial least squares regression (PLS-R) strategy, and the models were validated by 2 remaining normal batches. The determination coefficient of validation (R p 2 ), root mean square error of cross validation (RMSECV), root mean square error of prediction (RMSEP) and ratio of performance deviation (RPD) were 0.975, 0.501g/L, 0.465g/L and 5.57 for TP, and 0.969, 0.530g/L, 0.341g/L and 5.47 for HA, respectively. The results showed that the established models could give a rapid and accurate measurement of the content of TP and HA. The results of this study indicated that NIRS is an effective tool and could be successfully used for qualitative and quantitative monitoring the ethanol precipitation process of FI+II+III supernatant simultaneously. This research has significant reference value for assuring the quality and improving the recovery ratio of HA in industrialization scale by using NIRS. Copyright © 2016 Elsevier B.V. All rights reserved.
Tallinna Püha hõimkonna altariretaabel / Merike Koppel ; tõlk. Tiina Randviir, toim. Richard Adang

Index Scriptorium Estoniae

Koppel, Merike

2006-01-01

Tallinna Niguliste kirikus asuva 17. sajandi keskpaigas ümber tehtud hiliskeskaegse Püha hõimkonna altariretaabli algne pildiprogramm. Altariretaabli korpuse rekonstruktsioon. Kappaltari algse asukoha ja võimalike tellijate väljaselgitamisest. Püha hõimkond Flandria hiliskeskaegsetel nikerdaltaritel
PEP-II vacuum system pressure profile modeling using EXCEL

International Nuclear Information System (INIS)

Nordby, M.; Perkins, C.

1994-06-01

A generic, adaptable Microsoft EXCEL program to simulate molecular flow in beam line vacuum systems is introduced. Modeling using finite-element approximation of the governing differential equation is discussed, as well as error estimation and program capabilities. The ease of use and flexibility of the spreadsheet-based program is demonstrated. PEP-II vacuum system models are reviewed and compared with analytical models
HDPE/HA composites obtained in solution: Effect of the gamma radiation

International Nuclear Information System (INIS)

Carmen, Albano; Arquimedes, Karam; Rosestela, Perera; Gema, Gonzalez; Nohemy, Dominguez; Jeanette, Gonzalez; Yanixia, Sanchez

2006-01-01

Radiation is employed to sterilize composite materials used in the biomedical field. Due to the changes induced by radiation onto polymeric materials, it is important to study variations in their melt flow index (MFI), as well as in their mechanical and thermal properties. In this work, those previous parameters were determined in composites obtained via solution of a high-density polyethylene (HDPE) in decalin, with different amounts of hydroxyapatite (HA), varying from 10 to 30 parts per hundred, after being exposed to gamma radiation at absorbed doses between 25 and 100 kGy. After the irradiation, the MFI of HDPE dissolved in decalin and precipitated afterwards and without filler increased from 6 to 24 g/10 min at the highest absorbed doses. This behavior was also observed in composites with 10 pph of HA, being the increase less pronounced, specifically in the range between 50 and 100 kGy. Composites with 20 and 30 pph of HA showed a maximum MFI value at 50 kGy, which decreased at higher doses. This implies that the filler begin to exert an influence because it does not melt at the test temperature and consequently, it does not flow. It was observed that Young's modulus increased with HA addition due to rigidity of the ceramic filler. Radiation did not significantly affect this tensile property. On the other hand, the tensile strength did not show significant variations at the different doses but the filler content did affect this property improving it. Finally, elongation at break showed a drastic decrease with filler addition. When the thermal behavior was studied it was noticed that crystallization and melting temperatures remained unchanged. Instead, crystallinity degree slightly increased in the composites, and a little decrease was obtained when they were irradiated
Study on the changes of serum HA and CG levels in several diseases besides liver disorders

Energy Technology Data Exchange (ETDEWEB)

Zhiping, Lu; Yunbao, Ma; Xiaoyi, Zhang; Weihua, Lv [Changshu Hospital Attached to Medical College of Yangzhou Univ., Changshu No.2 People' s Hospital, Changshu (China)

2005-12-15

Objective: To study the changes of serum hyaluronic acid (HA) and cholyglycine (CG) in several diseases besides liver disorders. Methods: Serum HA and CG levels were measured with RIA in 78 patients with chronic liver diseases (with 70 controls), (84 patients with primary hepatic carcinoma ), 70 pediatric patients with various infections diseases (with 40 controls), 50 pediatric patients with recurrent respiratory infection (RRI, with 30 controls) and 428 pregnant women ( with 60 controls). In addition, RBC C{sub 3}b receptor ratio (RBCC{sub 3} bRR) and RBC immune-complex ratio (RBCICR) (both with yeast rosette method) as well as serum IgG,IgM,IgA,C{sub 3} levels (with immunoturbidity test) were examined in the 50 RRI pediatric patients, ALT levels were examined in the 70 pediatric patients with infectious diseases and SF examined in the 78 patients with chronic liver diseases. Results: The serum CG, HA and SF levels in the three groups of patients with chronic liver diseases ( CPH, CAH, Cirrhosis) were significantly higher than those in the controls (all P<0.01 ) with the exception of HA in CPH patients (P > 0.05). In patients with hepatic carcinoma, the CG and HA levels were positively correlated with the mortality at 4 months (P < 0.05). Among pregnant women, serum levels of HA and CG were significantly higher during the second and third trimesters (P<0.05-0.01) than those in the controls but not so during the first trimester. In the pediatric patients with infectious diseases, CG and HA levels were significantly higher in all these patients (P<0.01) with the only exception of HA in patients with mumps (P>0.05). For ALT, levels only increased in patients with hepatitis and typhoid fever with none in bacillary dysentery and mumps. In pediatric patients with RRI, RBCC{sub 3}bRR (%), CG, C{sub 3}, IgG levels were significantly higher than those in controls (P<0.05 ) with no significant differences in the case of RBCICR (%), HA, IgM and IgA levels (P>0
HA Cells monitoring at the Underground Research Laboratory (URL) in the CMHM (Andra)

International Nuclear Information System (INIS)

Gay, Olivier; Allagnat, Dominique; Morel, Jacques; Armand, Gilles

2010-01-01

The experimental monitoring program of the HA (High Activity) cells was carried out at the Underground Research Laboratory (URL) in the Meuse Haute Marne department in France (CMHM Andra). Inspections made by video and photographs, section measurements and geo-referenced trajectories, in addition to measurements of convergence, temperature and hygrometry over time, allowed a better analysis of the behaviour of the HA cells after excavation, and subsequently over the long term. (authors)
Augmenter le taux de vaccination et réduire la mortalité infantile à Haïti

International Development Research Centre (IDRC) Digital Library (Canada)

2 mai 2016 ... Résultats de l'Initiative canadienne d'immunisation internationale pour Haïti À Haïti, un enfant sur quatorze ne survit pas assez longtemps pour fêter son premier anniversaire. Parmi les facteurs contributifs, citons les taux extrêmement faibles de vaccination à Haïti, qui sont parmi les plus bas au monde.
Humic acid adsorption onto cationic cellulose nanofibers for bioinspired removal of copper( ii ) and a positively charged dye

KAUST Repository

Sehaqui, H.

2015-01-01

© The Royal Society of Chemistry. Waste pulp residues are herein exploited for the synthesis of a sorbent for humic acid (HA), which is a major water pollutant. Cellulose pulp was etherified with a quaternary ammonium salt in water thereby introducing positive charges onto the surface of the pulp fibers, and subsequently mechanically disintegrated into high surface area cellulose nanofibers (CNF). CNF with three different charge contents were produced and their adsorption capacity towards HA was investigated with UV-spectrophotometry, quartz crystal microbalance with dissipation, and ζ-potential measurements. Substantial coverage of the CNF surface with HA in a wide pH range led to a reversal of the positive ζ-potentials of CNF suspensions. The HA adsorption capacity and the kinetics of HA uptake were found to be promoted by both acidic pH conditions and the surface charge content of CNF. It is suggested that HA adsorption onto CNF depends on electrostatic interactions between the two components, as well as on the conformation of HA. At pH ∼ 6, up to 310 mg g^-1 of HA were adsorbed by the functionalized CNF, a substantially higher capacity than that of previously reported HA sorbents in the literature. It is further shown that CNF-HA complexes could be freeze-dried into "soil-mimicking" porous foams having good capacity to capture Cu(ii) ions and positive dyes from contaminated water. Thus, the most abundant natural polymer, i.e., cellulose could effectively bind the most abundant natural organic matter for environmental remediation purpose.
Physical and profile oceanographic data collected aboard the WEATHERBIRD II in the Gulf of Mexico from 2010-05-22 to 2010-05-27 in response to the Deepwater Horizon Oil Spill event (NODC Accession 0084597)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Physical and profile oceanographic data were collected aboard the WEATHERBIRD II in the Gulf of Mexico from 2010-05-22 to 2010-05-27 in response to the Deepwater...
PISCES and ALT-II: Juelich PSI papers

International Nuclear Information System (INIS)

Conn, R.W.; Hirooka, Y.; LaBombard, B.

1988-08-01

This publication comprises papers from the PISCES and ALT-II Programs at UCLA which were presented at the International Plasma Surface Interactions Meeting held in Juelich, FRG, on May 2-6, 1988. A list of publications from the PISCES and ALT-II contained in this report are: Deuterium pumping and erosion behavior of selected graphite materials under high flux plasma bombardment in PISCES; Erosion and redeposition behavior of selected NET-candidate materials under high-flux hydrogen, deuterium plasma bombardment in PISCES; Presheath profiles in simulated tokamak edge plasmas; Boundary asymmetries and plasma flow to the ALT-II toroidal belt pump limiter; ALT-II toroidal belt pump limiter performance in TEXTOR; and An in-situ spectroscopic erosion yield measurement with applications to sputtering and surface morphology alterations
Preparation and properties of polyvinyl alcohol (PVA) and hydroxylapatite (HA) hydrogels for cartilage tissue engineering.

Science.gov (United States)

Yuan, F; Ma, M; Lu, L; Pan, Z; Zhou, W; Cai, J; Luo, S; Zeng, W; Yin, F

2017-05-20

A novel bioactive hydrogel for cartilage tissue based on polyvinyl alcohol (PVA) and hydroxylapatite (HA) were prepared, the effects of its component contents on the mechanical properties and microstructure of the hydrogel were investigated. The important properties of the scaffold composites, such as density, porosity, compressive modulus and microstructure were studied and analyzed through various measurements and methods. The biodegradability of hydrogel was evaluated by soaking the samples into artificial degradation solution at body temperature (36 - 37 oC) in vitro. Experimental results showed that the PVA/HA hydrogels had a density of 0.572 - 0.683 g/cm3, a porosity of 63.25 - 96.14% and a compressive modulus of 5.62 - 8.24 MP. The HA compound in the hydrogels enhanced the biodegradation significantly and linearly increased the rate of biodegradation by 2.3 - 8.5 %. The compressive modulus of PVA/HA exhibited a linear reduce to 0.86 - 1.53 MP with the time of degradation. The scaffold composites PVA/HA possess a high porosity, decent compressive modulus and good biodegradability. After further optimizing the structure and properties, this composite might be considered as novel hydrogel biomaterials to be applied in the field of cartilage tissue engineering.
Characterization and corrosion property of nano-rod-like HA on fluoride coating supported on Mg-Zn-Ca alloy.

Science.gov (United States)

Feng, Yashan; Zhu, Shijie; Wang, Liguo; Chang, Lei; Yan, Bingbing; Song, Xiaozhe; Guan, Shaokang

2017-06-01

The poor corrosion resistance of biodegradable magnesium alloys is the dominant factor that limits their clinical application. In this study, to deal with this challenge, fluoride coating was prepared on Mg-Zn-Ca alloy as the inner coating and then hydroxyapatite (HA) coating as the outer coating was deposited on fluoride coating by pulse reverse current electrodeposition (PRC-HA/MgF 2 ). As a comparative study, the microstructure and corrosion properties of the composite coating with the outer coating fabricated by traditional constant current electrodeposition (TED-HA/MgF 2 ) were also investigated. Scanning electron microscopy (SEM) images of the coatings show that the morphology of PRC-HA/MgF 2 coating is dense and uniform, and presents nano-rod-like structure. Compared with that of TED-HA/MgF 2 , the corrosion current density of Mg alloy coated with PRC-HA/MgF 2 coatings decreases from 5.72 × 10 -5 A/cm 2 to 4.32 × 10 -7 A/cm 2 , and the corrosion resistance increases by almost two orders of magnitude. In immersion tests, samples coated with PRC-HA/MgF 2 coating always show the lowest hydrogen evolution amount, and could induce deposition of the hexagonal structure-apatite on the surface rapidly. The results show that the corrosion resistance and the bioactivity of the coatings have been improved by adopting double-pulse current mode in the process of preparing HA on fluoride coating, and the PRC-HA/MgF 2 coating is worth of further investigation.
Screening of the target genes trans-activated by HLA-HA8 in hepatocytes

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Qi WANG

2011-06-01

Full Text Available Objective To clone and identify the target genes trans-activated by human minor histocompatibility antigen HLA-HA8 in hepatocytes with suppression subtractive hybridization(SSH and bioinfomatics technique.Methods mRNA was isolated from HepG2 cells transfected by pcDNA3.1(--HLA-HA8 and pcDNA3.1(- empty vector,and then used to synthesize the double-stranded cDNA(marked as Tester and Driver,respectively by reverse transcription.After being digested with restriction enzyme Rsa I,the tester cDNA was divided into two parts and ligated to the specific adaptor 1 and adaptor 2,respectively,and then hybridized with driver cDNA twice and underwent PCR twice.The production was subcloned into pEGM-Teasy plasmid vectors to set up the subtractive library.The library was then amplified by transfection into E.coli strain DH5α.The cDNA was sequenced and analyzed in GenBank with Blast search after PCR amplification.Results The subtractive library of genes trans-activated by HLA-HA8 was constructed successfully.The amplified library contained 101 positive clones.Colony PCR showed that all these clones contained 200-1000bp inserts.Twenty eight clones were selected randomly to analyze the sequences.The result of homologous analysis showed that altogether 16 coding sequences were gotten,of which 4 sequences were with unknown function.Conclusions The obtained sequences trans-activated by HLA-HA8 may code different proteins and play important roles in cell growth and metabolism,energy synthesis and metabolism,material transport and signal transduction.This finding will bring some new clues for the studies not only on the biological functions of HLA-HA8,but also on the HBV infection mechanism.
CRED Shallow CTD Profiles; Rose Atoll, American Samoa; Cruise: HA1201_LEGII&III, Data Date Range: 20120419-20120422 (NODC Accession 0107470)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
CRED Shallow CTD Profiles; Ta'u, American Samoa; Cruise: HA1201_LEGII&III, Data Date Range: 20120422-20120423 (NODC Accession 0107470)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
Áreas metropolitanas:¿qué ha cambiado?: La experiencia de la Caracas Metropolitana

Directory of Open Access Journals (Sweden)

Sonia Barrios

2001-05-01

Full Text Available La nueva economía de la información y del conocimiento ha tenido tres decisivas implicaciones de orden territorial. Primero, ha acelerado el proceso de aglomeración urbana a escala planetaria. Segundo, ha provocado fuertes alteraciones en las redes urbanas de ámbito mundial, regional y nacional. Y tercero, ha impulsado la reestructuración interna de las áreas metropolitanas que se habían conformado durante la era industrial. Todo parece anticipar, por lo tanto, polarizaciones y fragmentaciones territoriales todavía más acentuadas que las conocidas en un pasado cercano. Tomando como referencia el caso de Caracas, se trata de averiguar en qué medida el desarrollo reciente de esta metrópoli sigue las grandes tendencias territoriales antes anotadas; y qué esfuerzos se están haciendo para ajustar sus instancias locales a las nuevas realidades urbanas. Como un recurso adicional, en distintos momentos se establecen comparaciones con Barcelona (España, considerada un ejemplo emblemático de las metrópolis de la era de la información

Removal of Uranium in Drinking Water: Brimac Environmental Services, Inc. Brimac HA 216 Adsorptive Media

Science.gov (United States)

The Brimac HA 216 Adsorptive Media was tested for uranium (U) removal from a drinking water source (well water) at Grappone Toyota located in Bow, New Hampshire. The HA 216 media is a hydroxyapatite-based material. A pilot unit, consisting of a TIGG Corporation Cansorb® C-5 ste...
Solvent extraction of Zn(II) from aqueous sulphate media by di(2-ethylhexyl) phosphoric acid in kerosene

International Nuclear Information System (INIS)

Begum, D.A.; Alauddin, M.; Rahman, M.S.

2009-01-01

The extraction equilibrium studies of Zn(II) from sulphate medium by di(2-ethylhexyl) phosphoric acid (D2EHPA, H/sub 2/A/sub 2/) in kerosene revealed that the distribution ratio (D) decreased with the increase of initial (Zn(A(II) in the aqueous phase and increased with the increase of equilibrium pH and extractant concentration. The equilibration is reached within 2 min. The species extracted into the organic phase is thought to be ZnA2, HA. The pH and extractant dependencies were about 2 and 1.67, respectively. The distribution ratio decreased with the increase in sulphate ion concentration in the aqueous phase. The extraction equilibrium reaction is suggested as Zn/sup 2+/ /sub aq/ +1.5 (H/sub 2/A/sub 2/)org - (ZnA/sub 2/, HA)org +2H/sup +/ /sub aq/. The extraction equilibrium constant (kex) for the above reaction was calculated to be 10-2.26. The extraction process was endothermic in nature having positive DH value of 16.27 kJ/mol. The loading of D2EHPA by Zn(II) is about 4.50 g of Zn(II) by 0.10 M D2EHPA. Possible reaction mechanism has been suggested based on distribution data, extractant concentration and equilibrium pH of the aqueous phase. (author)
Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from ATLANTIS II in the North Atlantic Ocean from 1981-06-12 to 1981-07-08 (NODC Accession 0117713)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117713 includes chemical, discrete sample, physical and profile data collected from ATLANTIS II in the North Atlantic Ocean from 1981-06-12 to...
24 CFR 964.135 - Resident involvement in HA management operations.

Science.gov (United States)

2010-04-01

... management operations. 964.135 Section 964.135 Housing and Urban Development Regulations Relating to Housing... Tenant Participation § 964.135 Resident involvement in HA management operations. Residents shall be... responsibility for management operations, it shall ensure strong resident participation in all issues and facets...
Microstructure, mechanical property and corrosion behavior of interpenetrating (HA + β-TCP)/MgCa composite fabricated by suction casting

International Nuclear Information System (INIS)

Wang, X.; Dong, L.H.; Li, J.T.; Li, X.L.; Ma, X.L.; Zheng, Y.F.

2013-01-01

The novel interpenetrating (HA + β-TCP)/MgCa composites were fabricated by infiltrating MgCa alloy into porous HA + β-TCP using suction casting technique. The microstructure, mechanical properties and corrosion behaviors of the composites have been evaluated by means of scanning electron microscopy (SEM), X-ray diffraction (XRD), mechanical testing, electrochemical and immersion tests. It was shown that the composites had compact structure and the interfacial bonding between MgCa alloy and HA + β-TCP scaffolds was very well. The ultimate compressive strength of the composites was about 500–1000 fold higher than that of the original porous scaffolds, and it still retained quarter-half of the strength of the bulk MgCa alloy. The electrochemical and immersion tests indicated that the corrosion resistance of the composites was better than that of the MgCa matrix alloy, and the corrosion products of the composite surface were mainly Mg(OH) 2 , HA and Ca 3 (PO 4 ) 2 . Meanwhile, the mechanical and corrosive properties of the (HA + β-TCP)/MgCa composites were adjustable by the choice of HA content. - Highlights: • The composites were fabricated by infiltrating MgCa alloy into porous HA + β-TCP. • The microstructure, mechanical and corrosion properties were investigated. • It showed composites had compact structures and good interfacial bonding. • The mechanical and corrosive properties can be adjustable by the HA content. • The corrosion mechanism of the composite has been explained
On the lack of correlation between Mg II 2796, 2803 Å and Lyα emission in lensed star-forming galaxies

Energy Technology Data Exchange (ETDEWEB)

Rigby, J. R. [Astrophysics Science Division, Goddard Space Flight Center, 8800 Greenbelt Road, Greenbelt, MD 20771 (United States); Bayliss, M. B. [Department of Physics, Harvard University, 17 Oxford Street, Cambridge, MA 02138 (United States); Gladders, M. D. [Department of Astronomy and Astrophysics, University of Chicago, 5640 S. Ellis Avenue, Chicago, IL 60637 (United States); Sharon, K. [Department of Astronomy, University of Michigan, 500 Church Street, Ann Arbor, MI 48109 (United States); Wuyts, E. [Max Plank Institute for Extraterrestrial Physics, Giessenbachstrasse 1, D-85748 Garching (Germany); Dahle, H. [Institute of Theoretical Astrophysics, University of Oslo, P.O. Box 1029, Blindern, NO-0315 Oslo (Norway)

2014-07-20

We examine the Mg II 2796, 2803 Å, Lyα, and nebular line emission in five bright star-forming galaxies at 1.66 < z < 1.91 that have been gravitationally lensed by foreground galaxy clusters. All five galaxies show prominent Mg II emission and absorption in a P Cygni profile. We find no correlation between the equivalent widths of Mg II and Lyα emission. The Mg II emission has a broader range of velocities than do the nebular emission line profiles; the Mg II emission is redshifted with respect to systemic by 100-200 km s{sup –1}. When present, Lyα is even more redshifted. The reddest components of Mg II and Lyα emission have tails to 500-600 km s{sup –1}, implying a strong outflow. The lack of correlation in the Mg II and Lyα equivalent widths, the differing velocity profiles, and the high ratios of Mg II to nebular line fluxes together suggest that the bulk of Mg II emission does not ultimately arise as nebular line emission, but may instead be reprocessed stellar continuum emission.
Description of rainfall variability in Br hat -samhita of Varâha-mihira

OpenAIRE

Iyengar, RN

2004-01-01

Br hat -samhita of Varâha-mihira (5–6th century AD) provides valuable information on the approach in ancient India towards monsoon rainfall, including its measurement and forecasting. In this context, we come across a description of the expected amount of total seasonal rainfall depending on the first rains under the 27 naks atras of Indian astronomy. This provides a rough statistical picture of what might have been the rainfall and its variability in the region around Ujjain, where Varâha-mi...
Synthesis and characterization of praseodymium-142 hydroxyapatite (142Pr-HA)

International Nuclear Information System (INIS)

Duyeh Setiawan; Daud Nurhasan

2015-01-01

The use of radioisotope of lanthanide group with range of beta energy 0.4 - 2.2 MeV has been renewed interest in nuclear medicine. Praseodymium-142 radioisotope ( 142 Pr, t 1/2 = 19.2 hours, E β = 2.16 MeV) is suitable for applications radiotherapy. Labelled of the hydroxyapatite (Ca 10 (PO 4 )6(OH) 2 ) can be used as carrier of radionuclide after injection in the body injection. This research aim as a preliminary studies to make praseodymium-142 hydroxyapatite ( 142 Pr-HA) as a radiotherapy agent. The optimum condition of praseodymium-142 hydroxyapatite synthesis by controlling several parameters such as the pH and the weight of hydroxyapatite was obtained from process by used the nonradioactive praseodymium. The optimum condition of hydroxyapatite by praseodymium are at pH 5 and weight ratio praseodymium : hydroxyapatite is 1 : 16. The percentage of labeling hydroxyapatite with 142 PrCl 3 was 99.50% and the radiochemical purity of 142 Pr-Ha was 95.20%. (author)
Una carta de pésame en arameo de Samuel ha-Naguid

Directory of Open Access Journals (Sweden)

Alba Cecilia, Amparo

2007-12-01

Full Text Available In this article we offer a Spanish translation with a commentary of an Aramaic letter of consolation written by Samuel ha-Naguid and sent to R. Ḥananel b. Ḥušiel, lamenting the death of R. Ḥušiel b. Elḥanán, his father.

En el presente artículo ofrecemos la traducción y el estudio de la carta de pésame enviada por Samuel ha-Naguid a R. Ḥananel b. Ḥušiel por la muerte de R. Ḥušiel b. Elḥanán, su padre.
Ulex europaeus agglutinin II (UEA-II) is a novel, potent inhibitor of complement activation.

Science.gov (United States)

Lekowski, R; Collard, C D; Reenstra, W R; Stahl, G L

2001-02-01

Complement is an important mediator of vascular injury following oxidative stress. We recently demonstrated that complement activation following endothelial oxidative stress is mediated by mannose-binding lectin (MBL) and activation of the lectin complement pathway. Here, we investigated whether nine plant lectins which have a binding profile similar to that of MBL competitively inhibit MBL deposition and subsequent complement activation following human umbilical vein endothelial cell (HUVEC) oxidative stress. HUVEC oxidative stress (1% O(2), 24 hr) significantly increased Ulex europaeus agglutinin II (UEA-II) binding by 72 +/- 9% compared to normoxic cells. UEA-II inhibited MBL binding to HUVEC in a concentration-dependent manner following oxidative stress. Further, MBL inhibited UEA-II binding to HUVEC in a concentration-dependent manner following oxidative stress, suggesting a common ligand. UEA-II (< or = 100 micromol/L) did not attenuate the hemolytic activity, nor did it inhibit C3a des Arg formation from alternative or classical complement pathway-specific hemolytic assays. C3 deposition (measured by ELISA) following HUVEC oxidative stress was inhibited by UEA-II in a concentration-dependent manner (IC(50) = 10 pmol/L). UEA-II inhibited C3 and MBL co-localization (confocal microscopy) in a concentration-dependent manner on HUVEC following oxidative stress (IC(50) approximately 1 pmol/L). Finally, UEA-II significantly inhibited complement-dependent neutrophil chemotaxis, but failed to inhibit fMLP-mediated chemotaxis, following endothelial oxidative stress. These data demonstrate that UEA-II is a novel, potent inhibitor of human MBL deposition and complement activation following human endothelial oxidative stress.
Physical and profile oceanographic data collected aboard NOAA Ship DELAWARE II in the Gulf of Mexico from 2010-06-26 to 2010-07-08 in response to the Deepwater Horizon Oil Spill event (NODC Accession 0084591)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Physical and profile oceanographic data were collected aboard NOAA Ship DELAWARE II in the Gulf of Mexico from 2010-06-26 to 2010-07-08 in response to the Deepwater...
Genetic alterations in Ki-ras and Ha-ras genes in Juvenile Nasopharyngeal Angiofibromas and head and neck cancer

Directory of Open Access Journals (Sweden)

Cláudia Malheiros Coutinho

1999-05-01

Full Text Available CONETXT: Ras gene mutations have been associated to a wide range of human solid tumors. Members of the ras gene family (Ki-ras, Ha-ras and N-ras are structurally related and code for a protein (p21 known to play an important role in the regulation of normal signal transduction and cell growth. The frequency of ras mutations is different from one type of tumor to another, suggesting that point mutations might be carcinogen-specific. OBJECTIVES: To study the occurrence of Ki-ras and Ha-ras mutations. We also studied the relative level of Ha-ras mRNA in 32 of the head and neck tumors. DESIGN: Case series. SETTING: University referral unit. PARTICIPANTS: 60 head and neck tumors and in 28 Juvenile Nasopharyngeal Angiofibromas (JNA. DIAGNOSTIC TEST: Using PCR-SSCP we examined the occurrence of Ki-ras and Ha-ras mutations. The relative level of Ha-ras mRNA was examined by Northern blot analysis. RESULTS: None of the head and neck tumors or JNA samples showed evidence of mutations within codons 12, 13, 59 and 61 of Ki-ras or Ha-ras genes. However, 17 (53% of the tumors where gene expression could be examined exhibited increased levels of Ha-ras mRNA compared with the normal tissue derived from the same patient. CONCLUSIONS: Our results demonstrate for the first time that mutations of Ki-ras and Ha-ras genes are not associated with the development of JNA and confirm previous reports indicating that activating ras mutations are absent or rarely involved in head and neck tumors from western world patients. Furthermore, our findings suggest that overexpression of Ha-ras, rather than mutations, might be an important factor in the development and progression of head and neck tumors.
Evolutionary Profiling of Group II Pyridoxal-Phosphate-Dependent Decarboxylases Suggests Expansion and Functional Diversification of Histidine Decarboxylases in Tomato

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Rahul Kumar

2016-03-01

Full Text Available Pyridoxal phosphate (PLP-dependent enzymes are one of the most important enzymes involved in plant N metabolism. Here, we explored the evolution of group II PLP-dependent decarboxylases (PLP_deC, including aromatic L-amino acid decarboxylase, glutamate decarboxylase, and histidine decarboxylase in the plant lineage. Gene identification analysis revealed a higher number of genes encoding PLP_deC in higher plants than in lower plants. Expression profiling of PLP_deC orthologs and syntelogs in (L. Heynh., pepper ( L., and tomato ( L. pointed toward conserved as well as distinct roles in developmental processes such as fruit maturation and ripening and abiotic stress responses. We further characterized a putative promoter of tomato ripening-associated gene ( operating in a complex regulatory circuit. Our analysis provides a firm basis for further in-depth exploration of the PLP_deC gene family, particularly in the economically important Solanaceae family.
An in vivo evaluation of PLLA/PLLA-gHA nano-composite for internal fixation of mandibular bone fractures.

Science.gov (United States)

Peng, Weihai; Zheng, Wei; Shi, Kai; Wang, Wangshu; Shao, Ying; Zhang, Duo

2015-11-09

Internal fixation of bone fractures using biodegradable poly(L-lactic-acid) (PLLA)-based materials has attracted the attention of many researchers. In the present study, 36 male beagle dogs were randomly assigned to two groups: PLLA/PLLA-gHA (PLLA-grafted hydroxyapatite) group and PLLA group. PLLA/PLLA-gHA and PLLA plates were embedded in the muscular bags of the erector spinae and also implanted to fix mandibular bone fractures in respective groups. At 1, 2, 3, 6, 9, and 12 months postoperatively, the PLLA/PLLA-gHA and PLLA plates were evaluated by adsorption and degradation tests, and the mandibles were examined through radiographic analysis, biomechanical testing, and histological analysis. The PLLA/PLLA-gHA plates were non-transparent and showed a creamy white color, and the PLLA plates were transparent and faint yellow in color. At all time points following surgery, adsorption and degradation of the PLLA/PLLA-gHA plates were significantly less than those of the PLLA plates, and the lateral and longitudinal bending strengths of the surgically treated mandibles of the beagle dogs in the PLLA/PLLA-gHA group were significantly greater than those of the PLLA group and reached almost the value of intact mandibles at 12 months postoperatively. Additionally, relatively rapid bone healing was observed in the PLLA/PLLA-gHA group with the formation of new lamellar bone tissues at 12 months after the surgery. The PLLA/PLLA-gHA nano-composite can be employed as a biodegradable material for internal fixation of mandibular bone fractures.
An in vivo evaluation of PLLA/PLLA-gHA nano-composite for internal fixation of mandibular bone fractures

International Nuclear Information System (INIS)

Peng, Weihai; Shi, Kai; Wang, Wangshu; Shao, Ying; Zhang, Duo; Zheng, Wei

2015-01-01

Internal fixation of bone fractures using biodegradable poly(L-lactic-acid) (PLLA)-based materials has attracted the attention of many researchers. In the present study, 36 male beagle dogs were randomly assigned to two groups: PLLA/PLLA-gHA (PLLA-grafted hydroxyapatite) group and PLLA group. PLLA/PLLA-gHA and PLLA plates were embedded in the muscular bags of the erector spinae and also implanted to fix mandibular bone fractures in respective groups. At 1, 2, 3, 6, 9, and 12 months postoperatively, the PLLA/PLLA-gHA and PLLA plates were evaluated by adsorption and degradation tests, and the mandibles were examined through radiographic analysis, biomechanical testing, and histological analysis. The PLLA/PLLA-gHA plates were non-transparent and showed a creamy white color, and the PLLA plates were transparent and faint yellow in color. At all time points following surgery, adsorption and degradation of the PLLA/PLLA-gHA plates were significantly less than those of the PLLA plates, and the lateral and longitudinal bending strengths of the surgically treated mandibles of the beagle dogs in the PLLA/PLLA-gHA group were significantly greater than those of the PLLA group and reached almost the value of intact mandibles at 12 months postoperatively. Additionally, relatively rapid bone healing was observed in the PLLA/PLLA-gHA group with the formation of new lamellar bone tissues at 12 months after the surgery. The PLLA/PLLA-gHA nano-composite can be employed as a biodegradable material for internal fixation of mandibular bone fractures. (paper)
Effect of iron II on hydroxyapatite dissolution and precipitation in vitro.

Science.gov (United States)

Delbem, A C B; Alves, K M R P; Sassaki, K T; Moraes, J C S

2012-01-01

The aim of this study was to evaluate the effect of iron II on the dissolution and precipitation of synthetic hydroxyapatite (HA). HA powder was suspended in solutions of iron (0.84 µg/ml, Fe0.84; 18.0 µg/ml, Fe18; 70.0 µg/ml, Fe70), fluoride (1,100 µg/ml, F1,100), and deionized water and submitted to pH cycling. After pH cycling, the samples were analyzed by infrared spectroscopy and X-ray diffraction. The concentrations of fluoride, calcium, phosphorus, and iron were also analyzed. The data were submitted to ANOVA, and analyzed by Tukey's test (p iron. The intensity of the phosphate bands increased and that of the hydroxyl bands decreased in the group F1,100. It was observed that there was a higher concentration of Ca in the group F1,100, with no significant difference between the groups Fe18 and Fe70 (p > 0.05). There was an increase in Fe concentration in the HA directly related to the Fe concentration of the treatment solutions. Results show that the presence of Fe causes the precipitation of apatite with high solubility. Copyright © 2012 S. Karger AG, Basel.
Antioxidant effects of betulin on porcine chondrocyte behavior in gelatin/C6S/C4S/HA modified tricopolymer scaffold

International Nuclear Information System (INIS)

Lin, Wen-Yang; Lin, Feng-Huei; Sadhasivam, S.; Savitha, S.

2010-01-01

The antioxidant effects of betulin on porcine chondrocytes cultured in gelatin/C6S/C4S/HA modified tricopolymer scaffold for a period of 4 weeks was investigated. The porous structure of the scaffold and cell attachment was observed by scanning electron microscopy (SEM). Biochemical measures of necrosis, cell proliferation, sulfated glycosaminoglycans (sGAG) content and extracellular matrix related gene expressions were quantitatively evaluated. The cell proliferation data showed good cellular viability in tricopolymer scaffold and increased optical density for total DNA demonstrated that the cells continued to proliferate inside the scaffold. The sGAG production indicated chondrogenic differentiation. Chondrocytes treated with betulin expressed transcripts encoding type II collagen, aggrecan, and decorin. To conclude, the substantiated results supported cell proliferation, production of extracellular matrix proteins and down-regulation of matrix metalloproteases and cytokine, in betulin treated scaffolds.
Antioxidant effects of betulin on porcine chondrocyte behavior in gelatin/C6S/C4S/HA modified tricopolymer scaffold

Energy Technology Data Exchange (ETDEWEB)

Lin, Wen-Yang; Lin, Feng-Huei [Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan (China); Sadhasivam, S., E-mail: rahulsbio@yahoo.co.in [Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan (China); Savitha, S. [Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan (China)

2010-05-10

The antioxidant effects of betulin on porcine chondrocytes cultured in gelatin/C6S/C4S/HA modified tricopolymer scaffold for a period of 4 weeks was investigated. The porous structure of the scaffold and cell attachment was observed by scanning electron microscopy (SEM). Biochemical measures of necrosis, cell proliferation, sulfated glycosaminoglycans (sGAG) content and extracellular matrix related gene expressions were quantitatively evaluated. The cell proliferation data showed good cellular viability in tricopolymer scaffold and increased optical density for total DNA demonstrated that the cells continued to proliferate inside the scaffold. The sGAG production indicated chondrogenic differentiation. Chondrocytes treated with betulin expressed transcripts encoding type II collagen, aggrecan, and decorin. To conclude, the substantiated results supported cell proliferation, production of extracellular matrix proteins and down-regulation of matrix metalloproteases and cytokine, in betulin treated scaffolds.
137Cs profiles in erosion plots with different soil cultivation

International Nuclear Information System (INIS)

Andrello, A.C.; Appoloni, C.R.; Cassol, E.A.; Melquiades, F.L.

2006-01-01

Cesium-137 methodology has been successfully used to assess soil erosion. Seven erosion plots were sampled to determine the 137 Cs profile and to assess the erosion rates. Cesium-137 profile for native pasture plot showed an exponential decline below 5 cm depth, with little 137 Cs activity in the superficial layer (0-5 cm). Cesium-137 profile for wheat-soybean rotation plot in conventional tillage showed a uniform distribution with depth. For this plot, the soil loss occurs more in middle than upper and lower level. Cesium-137 profile for wheat-soybean rotation and wheat-maize rotation plots in no-tillage showed a similar result to the native pasture, with a minimum soil loss in the superficial layer. Cesium-137 profile for bare soil and cultivated pasture plots are similar, with a soil erosion rate of 229 t ha -1 year -1 . In the plots with a conventional tillage a greater soil loss occur in middle than upper and lower level. In no-tillage cultivation plots occurs soil loss in lower level, but no sign of soil loss neither gain in the upper level is observed. Cesium-137 methodology is a good tool to assess soil erosion and the 137 Cs profile gives a possibility to understand the soil erosion behavior in erosion plots. (author)
Confinement studies in the TJ-II stellarator

International Nuclear Information System (INIS)

Alejaldre, C.; Alonso, J.; Almoguera, L.; Ascasibar, E.; Baciero, A.; Balbin, R.; Blaumoser, M.; Botija, J.; Branas, B.; Cal, E. de la; Cappa, A.; Carrasco, R.; Castejon, F.; Cepero, J.R.; Cremy, C.; Delgado, J.M.; Doncel, J.; Dulya, C.; Estrada, T.; Fernandez, A.; Fuentes, C.; Garcia, A.; Garcia-Cortes, I.; Guasp, J.; Herranz, J.; Hidalgo, C.; Jimenez, J.A.; Kirpitchev, I.; Krivenski, V.; Labrador, I.; Lapayese, F.; Likin, K.; Linier, M.; Lopez-Fraguas, A.; Lopez-Sanchez, A.; Luna, E. de la; Martin, R.; Martinez, A.; Martinez-Laso, L.; Medrano, M.; Mendez, P.; McCarthy, K.J.; Medina, F.; Milligen, B. van; Ochando, M.; Pacios, L.; Pastor, I.; Pedrosa, M.A.; Pena, A. de la; Portas, A.; Qin, J.; Rodriguez-Rodrigo, L.; Salas, A.; Sanchez, E.; Sanchez, J.; Tabares, F.; Tafalla, D.; Tribaldos, V.; Vega, J.; Zurro, B.; Akulina, D.; Fedyanin, O.I.; Grebenshchikov, S.; Kharchev, N.; Meshcheryakov, A.; Sarksian, K.A.; Barth, R.; Dijk, G. van; Meiden, H. van der

1999-01-01

ECR (electron cyclotron resonance) heated plasmas have been studied in the low magnetic shear TJ-II stellarator (R = 1.5 m, a ECRH = 300 kW, power density = 1-25 W cm -3 ). Recent experiments have explored the flexibility of the TJ-II across a wide range of plasma volumes with different rotational transforms and rational surface densities. In this paper, the main results of this campaign are presented and, in particular, the influence of iota and rational surfaces on plasma profiles is discussed. (author)

Fabrication of titanate nanotubes/iron oxide magnetic composite for the high efficient capture of radionuclides: a case investigation of 109Cd(II)

International Nuclear Information System (INIS)

Lei Dai; Jun Zheng; Lijie Wang

2013-01-01

In this paper, the capture of radiocadmium (Cd(II)) by adsorption onto the titanate nanotube/iron oxide (TNT/IOM) magnetic composite as a function of contact time, pH, ionic strength, foreign cation and anion ions, humic acid (HA) and fulvic acid (FA) was studied using batch technique. The results indicated that the adsorption of Cd(II) onto the TNT/IOM magnetic composite was dependent on ionic strength at pH 9.0. Outer-sphere surface complexation were the main mechanism of Cd(II) adsorption onto the TNT/IOM magnetic composite at low pH values, whereas the adsorption was mainly dominated via inner-sphere surface complexation at high pH values. The adsorption of Cd(II) onto the TNT/IOM magnetic composite was dependent on foreign cation and anion ions at low pH values, but was independent of foreign cation and anion ions at high pH values. A positive effect of HA/FA on Cd(II) adsorption onto the TNT/IOM magnetic composite was found at low pH values, while a negative effect was observed at high pH values. From the results of Cd(II) removal by the TNT/IOM magnetic composite, the optimum reaction conditions can be obtained for the maximum removal of Cd(II) from water. It is clear that the best pH values of the system to remove Cd(II) from solution by using the TNT/IOM magnetic composite are 7.0-8.0. Considering the low cost and effective disposal of Cd(II)-contaminated wastewaters, the best condition for Cd(II) capture by the TNT/IOM magnetic composite is at room temperature and solid content of 0.5 g L -1 . These results are quite important for estimating and optimizing the removal of Cd(II) and related metal ions by the TNT-based magnetic composite. (author)
Geophysical validation of SCIAMACHY Limb Ozone Profiles

Directory of Open Access Journals (Sweden)

E. J. Brinksma

2006-01-01

Full Text Available We discuss the quality of the two available SCIAMACHY limb ozone profile products. They were retrieved with the University of Bremen IFE's algorithm version 1.61 (hereafter IFE, and the official ESA offline algorithm (hereafter OL versions 2.4 and 2.5. The ozone profiles were compared to a suite of correlative measurements from ground-based lidar and microwave, sondes, SAGE II and SAGE III (Stratospheric Aerosol and Gas Experiment. To correct for the expected Envisat pointing errors, which have not been corrected implicitly in either of the algorithms, we applied a constant altitude shift of -1.5 km to the SCIAMACHY ozone profiles. The IFE ozone profile data between 16 and 40 km are biased low by 3-6%. The average difference profiles have a typical standard deviation of 10% between 20 and 35 km. We show that more than 20% of the SCIAMACHY official ESA offline (OL ozone profiles version 2.4 and 2.5 have unrealistic ozone values, most of these are north of 15° S. The remaining OL profiles compare well to correlative instruments above 24 km. Between 20 and 24 km, they underestimate ozone by 15±5%.
Beef production in irrigated and fertilized pastures of Brachiaria hybrid cv. Mulato II at the Sinú valley

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Sony Reza

2015-01-01

Full Text Available The main objective of this study was to evaluate the production of meat during the dry period in Brachiaria hybrid cv. Mulato II pasture with three stocking rates, irrigated and spring fertilized. The research was conducted for 140 days at the Center for Research Turipaná of the Colombian Corporation of Agricultural Research (Corpoica, located at Cereté, Córdoba (Colombia. Were grazed 48 cattle, commercial zebu with average weight of 339 ± 29 kg in an area of 12 hectares. Three treatments (3, 4, 5 animals/ha were evaluated with two replications in a randomized block design. Production, chemical and botanical composition, in situ degradability of dry matter and the daily weight gain was determined. Economic feasibility was determined by the benefit/cost ratio. The dry matter production was 1.925.16, 1.111.18 and 884.92 kg ha-1 for treatments 3, 4, 5 animal/ha respectively, detected differences (p0.05. For daily gain analysis found differences (p<0.05, with gains of 1.07, 0.989 and 0.940 kg animal for treatments 3, 4, 5 animal/ha. The treatment 5 animal/ha provided the best indicators economical.
Laminated electrospun nHA/PHB-composite scaffolds mimicking bone extracellular matrix for bone tissue engineering

Energy Technology Data Exchange (ETDEWEB)

Chen, Zhuoyue [Lab of Tissue Engineering, Faculty of Life Science, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province 710069 (China); Provincial Key Laboratory of Biotechnology of Shaanxi, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province 710069 (China); Song, Yue [Lab of Tissue Engineering, Faculty of Life Science, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province 710069 (China); Zhang, Jing [Lab of Tissue Engineering, Faculty of Life Science, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province 710069 (China); Provincial Key Laboratory of Biotechnology of Shaanxi, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province 710069 (China); Key Laboratory of Resource Biology and Modern Biotechnology in Western China, Ministry of Education, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province, 710069 (China); Liu, Wei [Lab of Tissue Engineering, Faculty of Life Science, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province 710069 (China); Cui, Jihong, E-mail: cjh@nwu.edu.cn [Lab of Tissue Engineering, Faculty of Life Science, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province 710069 (China); Provincial Key Laboratory of Biotechnology of Shaanxi, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province 710069 (China); Key Laboratory of Resource Biology and Modern Biotechnology in Western China, Ministry of Education, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province, 710069 (China); and others

2017-03-01

Electrospinning is an effective means to generate nano- to micro-scale polymer fibers resembling native extracellular matrix for tissue engineering. However, a major problem of electrospun materials is that limited pore size and porosity may prevent adequate cellular infiltration and tissue ingrowth. In this study, we first prepared thin layers of hydroxyapatite nanoparticle (nHA)/poly-hydroxybutyrate (PHB) via electrospinning. We then laminated the nHA/PHB thin layers to obtain a scaffold for cell seeding and bone tissue engineering. The results demonstrated that the laminated scaffold possessed optimized cell-loading capacity. Bone marrow mesenchymal stem cells (MSCs) exhibited better adherence, proliferation and osteogenic phenotypes on nHA/PHB scaffolds than on PHB scaffolds. Thereafter, we seeded MSCs onto nHA/PHB scaffolds to fabricate bone grafts. Histological observation showed osteoid tissue formation throughout the scaffold, with most of the scaffold absorbed in the specimens 2 months after implantation, and blood vessels ingrowth into the graft could be observed in the graft. We concluded that electrospun and laminated nanoscaled biocomposite scaffolds hold great therapeutic potential for bone regeneration. - Highlights: • We laminated the nHA/PHB layers to obtain a scaffold for bone tissue engineering. • The laminated scaffold performed optimized cell-loading capacity. • MSCs exhibited osteogenic phenotypes on the laminated scaffold. • Osteoid tissue formed throughout the laminated scaffold after 2 months in vivo. The laminated bio-composite scaffolds can be applied to bone regeneration.
Laminated electrospun nHA/PHB-composite scaffolds mimicking bone extracellular matrix for bone tissue engineering

International Nuclear Information System (INIS)

Chen, Zhuoyue; Song, Yue; Zhang, Jing; Liu, Wei; Cui, Jihong

2017-01-01

Electrospinning is an effective means to generate nano- to micro-scale polymer fibers resembling native extracellular matrix for tissue engineering. However, a major problem of electrospun materials is that limited pore size and porosity may prevent adequate cellular infiltration and tissue ingrowth. In this study, we first prepared thin layers of hydroxyapatite nanoparticle (nHA)/poly-hydroxybutyrate (PHB) via electrospinning. We then laminated the nHA/PHB thin layers to obtain a scaffold for cell seeding and bone tissue engineering. The results demonstrated that the laminated scaffold possessed optimized cell-loading capacity. Bone marrow mesenchymal stem cells (MSCs) exhibited better adherence, proliferation and osteogenic phenotypes on nHA/PHB scaffolds than on PHB scaffolds. Thereafter, we seeded MSCs onto nHA/PHB scaffolds to fabricate bone grafts. Histological observation showed osteoid tissue formation throughout the scaffold, with most of the scaffold absorbed in the specimens 2 months after implantation, and blood vessels ingrowth into the graft could be observed in the graft. We concluded that electrospun and laminated nanoscaled biocomposite scaffolds hold great therapeutic potential for bone regeneration. - Highlights: • We laminated the nHA/PHB layers to obtain a scaffold for bone tissue engineering. • The laminated scaffold performed optimized cell-loading capacity. • MSCs exhibited osteogenic phenotypes on the laminated scaffold. • Osteoid tissue formed throughout the laminated scaffold after 2 months in vivo. The laminated bio-composite scaffolds can be applied to bone regeneration.
Inference of RNA polymerase II transcription dynamics from chromatin immunoprecipitation time course data.

Directory of Open Access Journals (Sweden)

Ciira wa Maina

2014-05-01

Full Text Available Gene transcription mediated by RNA polymerase II (pol-II is a key step in gene expression. The dynamics of pol-II moving along the transcribed region influence the rate and timing of gene expression. In this work, we present a probabilistic model of transcription dynamics which is fitted to pol-II occupancy time course data measured using ChIP-Seq. The model can be used to estimate transcription speed and to infer the temporal pol-II activity profile at the gene promoter. Model parameters are estimated using either maximum likelihood estimation or via Bayesian inference using Markov chain Monte Carlo sampling. The Bayesian approach provides confidence intervals for parameter estimates and allows the use of priors that capture domain knowledge, e.g. the expected range of transcription speeds, based on previous experiments. The model describes the movement of pol-II down the gene body and can be used to identify the time of induction for transcriptionally engaged genes. By clustering the inferred promoter activity time profiles, we are able to determine which genes respond quickly to stimuli and group genes that share activity profiles and may therefore be co-regulated. We apply our methodology to biological data obtained using ChIP-seq to measure pol-II occupancy genome-wide when MCF-7 human breast cancer cells are treated with estradiol (E2. The transcription speeds we obtain agree with those obtained previously for smaller numbers of genes with the advantage that our approach can be applied genome-wide. We validate the biological significance of the pol-II promoter activity clusters by investigating cluster-specific transcription factor binding patterns and determining canonical pathway enrichment. We find that rapidly induced genes are enriched for both estrogen receptor alpha (ERα and FOXA1 binding in their proximal promoter regions.
Complications, disease profile and histological yield from ...

African Journals Online (AJOL)

Objective. The objective of the study was to evaluate (i) the technique used at Universitas Hospital in comparison with other international centres also performing renal biopsies, (ii) the disease profile in patients undergoing renal biopsies, (iii) the complications experienced during and/or after the procedure, and (iv) the ...
Two residues determine the product profile of the class II diterpene synthases TPS14 and TPS21 of Tripterygium wilfordii

DEFF Research Database (Denmark)

Hansen, Nikolaj Lervad; Nissen, Jakob N.; Hamberger, Björn Robert

2017-01-01

residue gave mixed product profiles. Two mutants, TwTPS14:Y265H and TwTPS21:A325V, also produced ent-copalyl diphosphate, highlighting the evolutionary potential of enzymes of this family to drive rapid diversification of plant diterpene biosynthesis through neo-functionalization. Our study contributes......The medicinal plant Tripterygium wilfordii (Celastraceae) contains a pair of class II diterpene synthases (diTPS) of specialized labdane-type metabolism that, despite remarkably close homology, form strikingly different products. TwTPS21 catalyzes bicyclization of the linear C20 precursor......-directed mutagenesis, we generated a panel of six variants, where one, or both positions were exchanged between the enzymes. In coupled heterologous assays with a corresponding class I diTPS, TwTPS2, complete product interchange was observed in variants with both reciprocal mutations, while substitutions of either...
Oxaliplatin loaded PLAGA microspheres: design of specific release profiles.

Science.gov (United States)

Lagarce, F; Cruaud, O; Deuschel, C; Bayssas, M; Griffon-Etienne, G; Benoit, J

2002-08-21

Oxaliplatin loaded PLAGA microspheres have been prepared by solvent extraction process. Parameters affecting the release kinetics in vitro have been studied in order to design specific release profiles suitable for direct intra-tumoral injection. By varying the nature and the relative proportions of different polymers we managed to prepare microspheres with good encapsulation efficiency (75-90%) and four different release profiles: zero order kinetics (type II) and the classical sigmoïd release profile with three different sizes of plateau and burst. These results, if correlated with in vivo activity, are promising to enhance effectiveness of local tumor treatment.
Soft tissue thickness of face profile conditioning by dento-skeletal anomalies

Directory of Open Access Journals (Sweden)

Tanić Tatjana

2011-01-01

Full Text Available Introduction. Orthodontic treatment of dento-skeletal anomalies is generally based on the correction of teeth and jaws relationship, while it is expected that soft facial tissue spontaneously adapts to therapeutically achieved relationship and to accompany hard tissue changes. Objective. To establish facial soft tissue thickness conditioning by the presence of dento-skeletal anomalies. Methods. The study was performed at the Dental Clinic of Niš, and involved the analysis of cephalometric rendgenograms in 121 patients, aged 12-18 years, with no previous orthodontical treatment. According to dento-skeletal relationship between teeth and jaws the patients were divided into four groups; class I (control group, class II of division 1, class II of division 2 and class III. The standard analysis of dento-skeletal profile was done according to Steiner and soft tissue profile according to Burstone was done in all. Results. The patients of class II/1 had a significantly thinner upper lip (t=2.650; p<0.05 and thinner upper lip sulcus (t=1.999; p<0.05. The patients of class II/2 had a significantly thicker upper lip (t=2.912; p<0.01, while those of class III had a significantly thinner lower lip (t=3.900; p<0.001. Conclusion. The thickness of facial soft tissue considerably influences facial profile appearance in persons with a dento-skeletal anomaly. Not only do soft tissues adapt to the existing jaws relationship, but can also camouflage present anomalies.
Combined orthodontic and surgical correction of adult skeletal class II with hyperdivergent jaws.

Science.gov (United States)

Abraham, Jiku; Bagchi, Paulami; Gupta, Swati; Gupta, Hemant; Autar, Ram

2012-01-01

A case of severe Class II skeletal malocclusion with anterior open bite having vertical growth pattern and matching soft tissue profile is presented. Considering age of the patient and the severity of the malocclusion, it was decided to combine orthodontic treatment with surgery. A 0.022 Roth Pre-adjusted Edgewise Appliance was chosen for the orthodontic correction and Le-Fort 1 differential vertical impaction of maxilla with mandibular autorotation and augmentation genioplasty was considered as the treatment plan. The main aim was to reduce the gummy smile and correct the class II profile.
La biblioteca del II Duque de Alburquerque (1467-1526

Directory of Open Access Journals (Sweden)

Ruiz García, Elisa

2002-06-01

Full Text Available Biographical profile of the II Duke of Alburquerque and detailed study of the books in his library through the list of the post mortem inventary of his possessions.

Perfil biográfico del II Duque de Alburquerque y estudio pormenorizado de los ejemplares de su biblioteca a través de la relación existente en el inventario port mortem de sus bienes.
An interpenetrating HA/G/CS biomimic hydrogel via Diels-Alder click chemistry for cartilage tissue engineering.

Science.gov (United States)

Yu, Feng; Cao, Xiaodong; Zeng, Lei; Zhang, Qing; Chen, Xiaofeng

2013-08-14

In order to mimic the natural cartilage extracellular matrix, a novel biological degradable interpenetrating network hydrogel was synthesized from the gelatin (G), hyaluronic acid (HA) and chondroitin sulfate (CS) by Diels-Alder "click" chemistry. HA was modified with furylamine and G was modified with furancarboxylic acid respectively. (1)H NMR spectra and elemental analysis showed that the substitution degrees of HA-furan and G-furan were 71.5% and 44.5%. Then the hydrogels were finally synthesized by cross-linking furan-modified HA and G derivatives with dimaleimide poly(ethylene glycol) (MAL-PEG-MAL). The mechanical and degradation properties of the hydrogels could be tuned simply through varying the molar ratio between furan and maleimide. Rheological, mechanical and degradation studies demonstrated that the Diels-Alder "click" chemistry is an efficient method for preparing high performance biological interpenetrating hydrogels. This biomimic hydrogel with improved mechanical properties could have great potential applications in cartilage tissue engineering. Copyright © 2013 Elsevier Ltd. All rights reserved.
Powder injection molding of HA/Ti6Al4V composite using palm stearin as based binder for implant material

International Nuclear Information System (INIS)

Arifin, Amir; Sulong, Abu Bakar; Muhamad, Norhamidi; Syarif, Junaidi; Ramli, Mohd Ikram

2015-01-01

Highlights: • Fabrication of HA/Ti6Al4V composite using powder injection molding. • Rheological results show that palm stearin is suitable as binder. • Resulted mechanical properties in between titanium alloy and HA values. • Micro porous enable accelerated bioactivity based on in vitro test. - Abstract: Titanium alloy (Ti6Al4V) and hydroxyapatite (HA) are well-known materials applied in implants. Ti6Al4V shows good mechanical properties and corrosion resistance, whereas HA possesses excellent biocompatibility and bioactivity but weak mechanical properties. The combination of the Ti6Al4V and HA properties is expected to produce a superior material for bio-implants. This study aimed to analyze the feasibility of fabricating HA/Ti6Al4V composites through powder injection molding (PIM) using palm stearin as base binder. In this study, 90 wt% Ti6Al4V and 10 wt% HA were mixed with the palm stearin and polyethylene binder system. The HA/Ti6Al4V feedstock showed pseudoplastic properties, suggesting its suitability for PIM. Flexural test revealed that the strength of the sintered composite ranges from 67.12 MPa to 112.97 MPa and its Young’s modulus ranges from 39.28 GPa to 44.25 GPa. The X-ray diffraction patterns and energy-dispersive X-ray spectra of the composite showed that the HA decomposed and formed secondary phases. Isotropic porous structure was observed on the sintered sample because of HA decomposition. Results showed that the palm stearin can be used as based binder in fabricating HA/Ti6Al4V composites via PIM. The mechanical properties of the sintered composites are nearly similar to those of the human bone. In addition, the increase in weight of the sintered composite during in vitro tests indicated the nucleation and growth of the Ca–P phase, which exhibited the biocompatibility of the fabricated HA/Ti6Al4V composite
Performance of the SURF-II high-throughput toroidal grating monochromator

International Nuclear Information System (INIS)

Kurtz, R.L.; Ederer, D.L.; Barth, J.; Stockbauer, R.

1988-01-01

The performance of the 'high-flux' toroidal grating monochromator (HFTGM) at the NBS SURF-II synchrotron storage ring is assessed. Two gratings are studied: One with a ruled profile and the other having a laminar profile. The laminar profile is shown to reduce substantially the intensity of higher-order diffracted light with only a small decrease in the intensity of the first order light. The dependence of the energy resolution as a function of the area of the grating illuminated is also discussed. (orig.)
CRED Shallow CTD Profiles; Johnston Atoll, Pacific Remote Island Areas; Cruise: HA1201_LEGI, Data Date Range: 20120302-20120304 (NODC Accession 0107470)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
CRED Shallow CTD Profiles; Jarvis Island, Pacific Remote Island Areas; Cruise: HA1201_LEGIV, Data Date Range: 20120503-20120505 (NODC Accession 0107470)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
CRED Shallow CTD Profiles; Howland Island, Pacific Remote Island Areas; Cruise: HA1201_LEGI, Data Date Range: 20120311-20120313 (NODC Accession 0107470)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
CRED Shallow CTD Profiles; Wake Atoll, Pacific Remote Island Areas; Cruise: HA1101_LEGI, Data Date Range: 20110322-20110325. (NODC Accession 0107470)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
CRED Shallow CTD Profiles; Baker Island, Pacific Remote Island Areas; Cruise: HA1201_LEGI, Data Date Range: 20120315-20120317 (NODC Accession 0107470)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...

CRED Shallow CTD Profiles; Hawai'i (Big Island), Main Hawaiian Islands; Cruise: HA1008, Data Date Range: 20101008-20101014 (NODC Accession 0039382).

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
L'Archivio del Concilio Vaticano II: storia e sviluppo.

OpenAIRE

Doria, P. (Piero)

2012-01-01

El artículo estudia los hechos que, después de la Asamblea Conciliar, han llevado a la reunificación en una única sede de los archivos de cada una de las Comisiones y Secretariados (1966-1967), a la institución de una Oficina del Archivo del Concilio Vaticano ii (1967), y a su reordenamiento y transferencia final en el Archivo Secreto Vaticano (2000), donde se ha realizado un largo trabajo de inventariado, aún en marcha, de la ingente documentación. Actualmente, están ...
Esterase profile of human masseter muscle

DEFF Research Database (Denmark)

Kirkeby, S; Moe, D; Vilmann, H

1988-01-01

The esterase profile of fresh human masseter muscle was investigated by use of histochemistry and electrophoresis. The histochemical methods included reactions for alpha-naphthyl esterase, myofibrillar ATPase, reverse myofibrillar ATPase and succinic dehydrogenase. In frozen sections of the muscle...... the coloured reaction product for esterases was present both as a diffuse sarcoplasmic coloration and as distinct granules. The intensity of diffuse reaction was used to classify the muscle fibres as strongly, moderately and weakly reacting. The fibres with strong esterase activity belonged to Type I and ii......C. iM and Type II A fibres showed a moderate esterase reaction and Type II B fibres had a low activity. The electrophoretic gels stained for esterase activity showed that the human masseter muscle possesses a slow migrating double band with high enzyme activity and a cascade of faster migrating...
Laminated electrospun nHA/PHB-composite scaffolds mimicking bone extracellular matrix for bone tissue engineering.

Science.gov (United States)

Chen, Zhuoyue; Song, Yue; Zhang, Jing; Liu, Wei; Cui, Jihong; Li, Hongmin; Chen, Fulin

2017-03-01

Electrospinning is an effective means to generate nano- to micro-scale polymer fibers resembling native extracellular matrix for tissue engineering. However, a major problem of electrospun materials is that limited pore size and porosity may prevent adequate cellular infiltration and tissue ingrowth. In this study, we first prepared thin layers of hydroxyapatite nanoparticle (nHA)/poly-hydroxybutyrate (PHB) via electrospinning. We then laminated the nHA/PHB thin layers to obtain a scaffold for cell seeding and bone tissue engineering. The results demonstrated that the laminated scaffold possessed optimized cell-loading capacity. Bone marrow mesenchymal stem cells (MSCs) exhibited better adherence, proliferation and osteogenic phenotypes on nHA/PHB scaffolds than on PHB scaffolds. Thereafter, we seeded MSCs onto nHA/PHB scaffolds to fabricate bone grafts. Histological observation showed osteoid tissue formation throughout the scaffold, with most of the scaffold absorbed in the specimens 2months after implantation, and blood vessels ingrowth into the graft could be observed in the graft. We concluded that electrospun and laminated nanoscaled biocomposite scaffolds hold great therapeutic potential for bone regeneration. Copyright © 2016 Elsevier B.V. All rights reserved.
A real-time current driving control system for the TJ-II coils

International Nuclear Information System (INIS)

Pena, Angel de la; Pacios, Luis; Carrasco, Ricardo; Lapayese, Fernando

2009-01-01

Since the start of plasma operation in the TJ-II stellarator, the required values of DC currents that are fed to its different coil sets have been controlled to high precision along the complete discharge flat-top. As a result each current configuration produced a highly stable magnetic-field configuration. Recently, the configurational flexibility of the TJ-II has been broadened by the commissioning of a new mode of operation that allows magnetic configurations to be varied dynamically during the discharge flat-top. In order to achieve this, new hardware and software features have been added to the TJ-II Control System. These new features may also provide new strategies for feedback control in accordance with parameters measured in one or more diagnostics. In this new set-up, coil current profiles are generated and controlled to millisecond timescales by a system based on VMEbus and OS9 real-time operating system. A new communication middleware architecture called XML-based Messages Distribution Service (X-MDS) has been designed to exchange XML-based data with calling clients. Furthermore, with this software, a fully functional Java application for supervision and for current profiles settings has been developed. This paper provides a detailed description of the complete TJ-II real-time current-profile control system and results obtained during its operation.
A real-time current driving control system for the TJ-II coils

Energy Technology Data Exchange (ETDEWEB)

Pena, Angel de la [Association EURATOM - CIEMAT, Avda. Complutense 22, 28040 Madrid (Spain)], E-mail: a.delapena@ciemat.es; Pacios, Luis; Carrasco, Ricardo; Lapayese, Fernando [Association EURATOM - CIEMAT, Avda. Complutense 22, 28040 Madrid (Spain)

2009-06-15

Since the start of plasma operation in the TJ-II stellarator, the required values of DC currents that are fed to its different coil sets have been controlled to high precision along the complete discharge flat-top. As a result each current configuration produced a highly stable magnetic-field configuration. Recently, the configurational flexibility of the TJ-II has been broadened by the commissioning of a new mode of operation that allows magnetic configurations to be varied dynamically during the discharge flat-top. In order to achieve this, new hardware and software features have been added to the TJ-II Control System. These new features may also provide new strategies for feedback control in accordance with parameters measured in one or more diagnostics. In this new set-up, coil current profiles are generated and controlled to millisecond timescales by a system based on VMEbus and OS9 real-time operating system. A new communication middleware architecture called XML-based Messages Distribution Service (X-MDS) has been designed to exchange XML-based data with calling clients. Furthermore, with this software, a fully functional Java application for supervision and for current profiles settings has been developed. This paper provides a detailed description of the complete TJ-II real-time current-profile control system and results obtained during its operation.
Consistencia interna del cuestionario autoadministrado de la Entrevista Clínica Estructurada para Trastornos del Eje II del DSM-IV*

OpenAIRE

Campo-Arias, Adalberto; Díaz-Martínez, Luis Alfonso; Barros-Bermúdez, Jaider Alfonso

2008-01-01

Introducción: Es infrecuente identificar posibles casos de trastornos de personalidad en la población general, debido a la carencia de instrumentos autoadministrados con buena validez y confiabilidad. Hasta la fecha no se ha explorado la confiabilidad del cuestionario autoadministrado de la Entrevista Clínica Estructurada para Diagnósticos del eje II (SDID-II) del DSM-IV en la población colombiana. Objetivo: Determinar la consistencia interna de las subescalas del cuestionario autoadministrad...
Transgenic Cotton Plants Expressing the HaHR3 Gene Conferred Enhanced Resistance to Helicoverpa armigera and Improved Cotton Yield.

Science.gov (United States)

Han, Qiang; Wang, Zhenzhen; He, Yunxin; Xiong, Yehui; Lv, Shun; Li, Shupeng; Zhang, Zhigang; Qiu, Dewen; Zeng, Hongmei

2017-08-30

RNA interference (RNAi) has been developed as an efficient technology. RNAi insect-resistant transgenic plants expressing double-stranded RNA (dsRNA) that is ingested into insects to silence target genes can affect the viability of these pests or even lead to their death. HaHR3 , a molt-regulating transcription factor gene, was previously selected as a target expressed in bacteria and tobacco plants to control Helicoverpa armigera by RNAi technology. In this work, we selected the dsRNA- HaHR3 fragment to silence HaHR3 in cotton bollworm for plant mediated-RNAi research. A total of 19 transgenic cotton lines expressing HaHR3 were successfully cultivated, and seven generated lines were used to perform feeding bioassays. Transgenic cotton plants expressing ds HaHR3 were shown to induce high larval mortality and deformities of pupation and adult eclosion when used to feed the newly hatched larvae, and 3rd and 5th instar larvae of H. armigera . Moreover, HaHR3 transgenic cotton also demonstrated an improved cotton yield when compared with controls.
Differential cognitive profiles of intimate partner violence perpetrators based on alcohol consumption.

Science.gov (United States)

Vitoria-Estruch, Sara; Romero-Martínez, Angel; Lila, Marisol; Moya-Albiol, Luis

2018-08-01

Despite extensive evidence of heterogeneity in intimate partner violence (IPV) perpetrator profiles, there has been little research into neuropsychological deficits that might help us understand differences within this violent population. Moreover, studies on this topic have not paid much attention to the role of alcohol abuse in neuropsychological domains of IPV perpetrators. Hence, the current study was designed to examine neuropsychological differences among individuals who have committed domestic violence with high (n = 28, HA) and low (n = 35, LA) levels of alcohol consumption, and non-violent individuals (n = 37) to establish differential neuropsychological profiles. An exhaustive neuropsychological assessment battery was employed which combined the computer-based Cambridge Neuropsychological Test Automated Battery with pencil-and-paper measures. Compared to controls, HA IPV perpetrators had slower processing speed and significantly more impairments in attentional set-shifting or switch attention, working and long-term memory, cognitive flexibility, planning, decision-making, emotion decoding skills, and perspective taking. Furthermore, there were differences between IPV perpetrator subgroups in attentional set-shifting or switch attention and cognitive empathy, with HA IPV perpetrators displaying more severe impairments in both cognitive domains than LA IPV perpetrators. Finally, the LA IPV perpetrators had significantly more impairments in working and long-term memory, executive functioning, and emotion decoding skills than controls, but they did not differ in processing speed, attentional set-shifting or switch attention, decision making, or perspective taking. Thus, the current findings suggest that IPV perpetrators with neuropsychological difficulties, especially those who are heavy drinkers, may have the greatest need for cognitive interventions. These cognitive deficits could be employed as targets for developing specific cognitive rehabilitation
Quantifying soil profile change caused by land use in central Missouri loess hillslopes

Science.gov (United States)

Samuel J. Indorante; John M. Kabrick; Brad D. Lee; Jon M. Maatta

2014-01-01

Three major challenges are present when studying anthropogenic impacts on soil profile properties: (i) site selection; (ii) sampling and modeling native and cultivated soil-landscape relationships; and (iii) graphically and statistically comparing native and cultivated sites to model soil profile changes. This study addressed those challenges by measuring and modeling...
Hyaluronic acid doped-poly(3,4-ethylenedioxythiophene)/chitosan/gelatin (PEDOT-HA/Cs/Gel) porous conductive scaffold for nerve regeneration

International Nuclear Information System (INIS)

Wang, Shuping; Guan, Shui; Zhu, Zhibo; Li, Wenfang; Liu, Tianqing; Ma, Xuehu

2017-01-01

Conducting polymer, as a “smart” biomaterial, has been increasingly used to construct tissue engineered scaffold for nerve tissue regeneration. In this study, a novel porous conductive scaffold was prepared by incorporating conductive hyaluronic acid (HA) doped-poly(3,4-ethylenedioxythiophene) (PEDOT-HA) nanoparticles into a chitosan/gelatin (Cs/Gel) matrix. The physicochemical characteristics of Cs/Gel scaffold with 0–10 wt% PEDOT-HA were analyzed and the results indicated that the incorporation of PEDOT-HA into scaffold increased the electrical and mechanical properties while decreasing the porosity and water absorption. Moreover, in vitro biodegradation of scaffold displayed a declining trend with the PEDOT-HA content increased. About the biocompatibility of conductive scaffold, neuron-like rat phaeochromocytoma (PC12) cells were cultured in scaffold to evaluate cell adhesion and growth. 8% PEDOT-HA/Cs/Gel scaffold had a higher cell adhesive efficiency and cell viability than the other conductive scaffolds. Furthermore, cells in the scaffold with 8 wt% PEDOT-HA expressed higher synapse growth gene of GAP43 and SYP compared with Cs/Gel control group. These results suggest that 8%PEDOT-HA/Cs/Gel scaffold is an attractive cell culture conductive substrate which could support cell adhesion, survival, proliferation, and synapse growth for the application in nerve tissue regeneration. - Highlights: • A porous conductive scaffold was prepared by freeze-drying method. • Conductive scaffold could support PC12 cells adhesion, survival, and proliferation. • Cells in conductive scaffold expressed high synapse growth gene of GAP43 and SYP.
Hyaluronic acid doped-poly(3,4-ethylenedioxythiophene)/chitosan/gelatin (PEDOT-HA/Cs/Gel) porous conductive scaffold for nerve regeneration

Energy Technology Data Exchange (ETDEWEB)

Wang, Shuping; Guan, Shui, E-mail: guanshui@dlut.edu.cn; Zhu, Zhibo; Li, Wenfang; Liu, Tianqing; Ma, Xuehu

2017-02-01

Conducting polymer, as a “smart” biomaterial, has been increasingly used to construct tissue engineered scaffold for nerve tissue regeneration. In this study, a novel porous conductive scaffold was prepared by incorporating conductive hyaluronic acid (HA) doped-poly(3,4-ethylenedioxythiophene) (PEDOT-HA) nanoparticles into a chitosan/gelatin (Cs/Gel) matrix. The physicochemical characteristics of Cs/Gel scaffold with 0–10 wt% PEDOT-HA were analyzed and the results indicated that the incorporation of PEDOT-HA into scaffold increased the electrical and mechanical properties while decreasing the porosity and water absorption. Moreover, in vitro biodegradation of scaffold displayed a declining trend with the PEDOT-HA content increased. About the biocompatibility of conductive scaffold, neuron-like rat phaeochromocytoma (PC12) cells were cultured in scaffold to evaluate cell adhesion and growth. 8% PEDOT-HA/Cs/Gel scaffold had a higher cell adhesive efficiency and cell viability than the other conductive scaffolds. Furthermore, cells in the scaffold with 8 wt% PEDOT-HA expressed higher synapse growth gene of GAP43 and SYP compared with Cs/Gel control group. These results suggest that 8%PEDOT-HA/Cs/Gel scaffold is an attractive cell culture conductive substrate which could support cell adhesion, survival, proliferation, and synapse growth for the application in nerve tissue regeneration. - Highlights: • A porous conductive scaffold was prepared by freeze-drying method. • Conductive scaffold could support PC12 cells adhesion, survival, and proliferation. • Cells in conductive scaffold expressed high synapse growth gene of GAP43 and SYP.
Silencing of HaAce1 gene by host-delivered artificial microRNA disrupts growth and development of Helicoverpa armigera.

Science.gov (United States)

Saini, Ravi Prakash; Raman, Venkat; Dhandapani, Gurusamy; Malhotra, Era Vaidya; Sreevathsa, Rohini; Kumar, Polumetla Ananda; Sharma, Tilak R; Pattanayak, Debasis

2018-01-01

The polyphagous insect-pest, Helicoverpa armigera, is a serious threat to a number of economically important crops. Chemical application and/or cultivation of Bt transgenic crops are the two strategies available now for insect-pest management. However, environmental pollution and long-term sustainability are major concerns against these two options. RNAi is now considered as a promising technology to complement Bt to tackle insect-pests menace. In this study, we report host-delivered silencing of HaAce1 gene, encoding the predominant isoform of H. armigera acetylcholinesterase, by an artificial microRNA, HaAce1-amiR1. Arabidopsis pre-miRNA164b was modified by replacing miR164b/miR164b* sequences with HaAce1-amiR1/HaAce1-amiR1* sequences. The recombinant HaAce1-preamiRNA1 was put under the control of CaMV 35S promoter and NOS terminator of plant binary vector pBI121, and the resultant vector cassette was used for tobacco transformation. Two transgenic tobacco lines expressing HaAce1-amiR1 was used for detached leaf insect feeding bioassays. Larval mortality of 25% and adult deformity of 20% were observed in transgenic treated insect group over that control tobacco treated insect group. The reduction in the steady-state level of HaAce1 mRNA was 70-80% in the defective adults compared to control. Our results demonstrate promise for host-delivered amiRNA-mediated silencing of HaAce1 gene for H. armigera management.
Weathering of the New Albany Shale, Kentucky: II. Redistribution of minor and trace elements

Science.gov (United States)

Tuttle, M.L.W.; Breit, G.N.; Goldhaber, M.B.

2009-01-01

During weathering, elements enriched in black shale are dispersed in the environment by aqueous and mechanical transport. Here a unique evaluation of the differential release, transport, and fate of Fe and 15 trace elements during progressive weathering of the Devonian New Albany Shale in Kentucky is presented. Results of chemical analyses along a weathering profile (unweathered through progressively weathered shale to soil) describe the chemically distinct pathways of the trace elements and the rate that elements are transferred into the broader, local environment. Trace elements enriched in the unweathered shale are in massive or framboidal pyrite, minor sphalerite, CuS and NiS phases, organic matter and clay minerals. These phases are subject to varying degrees and rates of alteration along the profile. Cadmium, Co, Mn, Ni, and Zn are removed from weathered shale during sulfide-mineral oxidation and transported primarily in aqueous solution. The aqueous fluxes for these trace elements range from 0.1 g/ha/a (Cd) to 44 g/ha/a (Mn). When hydrologic and climatic conditions are favorable, solutions seep to surface exposures, evaporate, and form Fe-sulfate efflorescent salts rich in these elements. Elements that remain dissolved in the low pH (pH. Neutralization of the weathering solution in local streams results in elements being adsorbed and precipitated onto sediment surfaces, resulting in trace element anomalies. Other elements are strongly adsorbed or structurally bound to solid phases during weathering. Copper and U initially are concentrated in weathering solutions, but become fixed to modern plant litter in soil formed on New Albany Shale. Molybdenum, Pb, Sb, and Se are released from sulfide minerals and organic matter by oxidation and accumulate in Fe-oxyhydroxide clay coatings that concentrate in surface soil during illuviation. Chromium, Ti, and V are strongly correlated with clay abundance and considered to be in the structure of illitic clay. Illite
Clinical significance of determination of changes of serum HA, IL-2 and TNF-α contents in patients with psoriasis

International Nuclear Information System (INIS)

Ren Hong

2006-01-01

Objective: To investigate the changes of serum HA, IL-2 and TNF-α contents in patients with psoriasis. Methods: Serum HA, TNF-α levels were measured with RIA and IL-2 levels was measured with ELISA in 47 patients with psoriasis as well as 35 controls. Results: The serum HA, TNF-α levels were significantly higher in the patients than those in controls (P < 0. 01), while the serum IL-2 levels were significantly lower (P<0.01). Conclusion: Determination of serum HA, IL-2 and TNF-α contents would be clinically useful for understanding the disturbances of immunomodulation in these patients. (authors)
Perawatan Maloklusi Angle Klas II Divisi 1 Menggunakan Bionator Myofungsional

Directory of Open Access Journals (Sweden)

Ragil Irawan

2014-06-01

Full Text Available Maloklusi Angle klas II divisi 1 mempunyai ciri tonjol mesiobukal molar pertama atas beroklusi dengan interdental premolar kedua dan molar pertama bawah, jarak gigit yang besar, lengkung gigi sempit dan profil cembung. Bionator pertama kali diperkenalkan oleh Balter dan merupakan alat ortodontik myofungsional yang digunakan untuk merawat diskrepansi rahang. Tujuan pemaparan kasus adalah menyajikan kemajuan kasus maloklusi Angle Klas II divisi 1 disertai diskrepansi rahang menggunakan alat myofunctional bionator. Seorang perempuan berusia 13 tahun mengeluhkan gigi depan atas maju. Diagnosis pasien maloklusi Angle klas II divisi 1, hubungan skeletal klas II dengan protrusif maksila dan retrusif mandibula, protrusif insisivus atas disertai palatal bite, cross bite posterior, jarak gigit 11 mm, tumpang gigit 5,25 mm, SNA 84°, SNB 76°. Pasien dirawat menggunakan alat myofungsional bionator. Hasil perawatan setelah satu tahun overjet menjadi 6,25 mm dan SNB 78°. Kesimpulannya adalah alat myofungsional bionator efektif untuk merawat maloklusi Angle Klas II divisi 1 yang disertai diskrepansi rahang. Class II division 1 Angle Malocclussion Treatment Using Myofunctional Bionator. Malocclusion Angle Class II division I is characterized by the upper mesio-buccal cups first permanent molars occludes in interdental second bicuspid and lower first molar permanent, increased overjet, narrow arch form, and convex profile. Bionator originally developed by Balter and used to treat jaw discrepancy. The goal of this case is to present the progress of myofunctional bionator appliance in treating malocclusion Class II division 1 with jaw discrepancies. A Female 13 years old complained protrusive anterior teeth. Diagnosis is malocclusion Angle Class II division I, class II skeletal relationship with maxilla protrusive, mandible retrusive, protrusive upper incisor, palatal bite, posterior cross bite, overjet 11 mm, overbite 5,25 mm SNA 84°, SNB 76�
pH-Amplified multilayer films based on hyaluronan: influence of HA molecular weight and concentration on film growth and stability.

Science.gov (United States)

Shen, Liyan; Chaudouet, Patrick; Ji, Jian; Picart, Catherine

2011-04-11

In this study, we investigate the growth and internal properties of polyelectrolyte multilayer films made of poly(l-lysine) and hyaluronan (PLL/HA) under pH-amplified conditions, that is, by alternate deposition of PLL at high pH and HA at low pH. We focus especially on the influence of the molecular weight of HA in this process as well as on its concentration in solution. Film growth was followed by quartz crystal microbalance and by infrared spectroscopy to quantify the deposited mass and to characterize the internal properties of the films, including the presence of hydrogen bonds and the ionization degree of HA in the films. Film growth was significantly faster for HA of high molecular weight (1300 kDa) as compared with 400 and 200 kDa. PLL was found to exhibit a random structure once deposited in the films. Furthermore, we found that PLL-ending films are more stable when they are placed in PBS than their HA counterparts. This was explained on the basis of more cohesive interactions in the films for PLL-ending films. Finally, we quantified PLL(FITC) diffusion into the films and observed that PLL diffusion is enhanced when PLL is paired with the HA of high MW. All together, these results suggest that besides purely physicochemical parameters such as variation in pH, the molecular weight of HA, its concentration in solution, and the possibility to form intermolecular HA association play important roles in film growth, internal cohesion, and stability.
Laser fabrication of Ag-HA nanocomposites on Ti6Al4V implant for enhancing bioactivity and antibacterial capability

Energy Technology Data Exchange (ETDEWEB)

Liu, Xiangmei; Man, H.C., E-mail: mfhcman@polyu.edu.hk

2017-01-01

For titanium alloy implants, both surface bioactivity and antibacterial infection are the two critical factors in determining the success of clinical implantation of these metallic implants. In the present work, a novel nanocomposite layer of nano-silver-containing hydroxyapatite (Ag-HA) was prepared on the surface of biomedical Ti6Al4V by laser processing. Analysis using SEM, EDS and XRD shows the formation of an Ag-HA layer of about 200 μm fusion bonded to the substrate. Mineralization tests in simulated body fluid (SBF) showed that laser fabricated Ag-HA nanocomposite layer favors the deposition of apatite on the surface of the implants. Antibacterial tests confirmed that all Ag-HA nanocomposite layers can kill bacteria while a higher Ag content would lower the cytocompatibility of these coatings. Cell viability decreases when the Ag content reaches 5% in these coatings, due to the larger amount of Ag leached out, as confirmed by ion release evaluation. Our results reveal that laser fabricated Ag-HA nanocomposite coatings containing 2% Ag show both excellent cytocompatibility and antibacterial capability. - Highlights: • Silver-containing hydroxyapatite (Ag-HA) nanocomposite layer was fabricated on Ti6Al4V by laser technique. • Both bioactivity and antibacterial capability were significantly enhanced compared with bare Ti6Al4V. • Ag-HA nanocomposite coatings containing 2% Ag show both excellent cytocompatibility and antibacterial capability.
Effects of DCPD cement chemistry on degradation properties and cytocompatibility: comparison of MCPM/β-TCP and MCPM/HA formulations

International Nuclear Information System (INIS)

Alge, Daniel L; Goebel, W Scott; Chu, Tien-Min Gabriel

2013-01-01

Dicalcium phosphate dihydrate (DCPD) cements are attractive biomaterials for bone repair, and a number of different DCPD cement formulations have been proposed in the literature. In this study, we have specifically compared monocalcium phosphate monohydrate (MCPM)/hydroxyapatite (HA) and MCPM/β-tricalcium phosphate (β-TCP) formulations to test the hypothesis that DCPD cement chemistry affects the degradation properties and cytocompatibility of the cement. Using simple in vitro models we found that MCPM/β-TCP formulations degraded primarily by DCPD dissolution, which was associated with a slight pH drop and relatively low mass loss. Cytocompatibility testing of cement conditioned culture media revealed no significant change in cell viability relative to the negative control for all of the MCPM/β-TCP formulations. In contrast, the MCPM/HA formulations were prone to undergo rapid conversion of DCPD to HA, resulting in a sharp pH drop and extensive mass loss. A stoichiometric excess of HA in the cement was found to accelerate the conversion process, and significant cytotoxicity was observed for the MCPM/HA formulations containing excess HA. Collectively, these results show that, although the product of the setting reaction is the same, DCPD cements produced with MCPM/HA and MCPM/β-TCP formulations differ significantly in their degradation properties and cytocompatibility. These differences may have important implications for the selection of a DCPD cement formulation for clinical application. (paper)
Production of polyclonal antibody against Tehran strain influenza virus (A/H1N1/2009 hemagglutinin conserved domain (HA2: brief report

Directory of Open Access Journals (Sweden)

Somayeh Zamani

2015-10-01

Full Text Available Background: The influenza virus is one of the most important factors for higher morbidity and mortality in the world. Recently, researchers have been focused on influenza conserved antigenic proteins such as hemagglutinin stalk domain (HA2 for vaccine production and serological studies. The HA2 plays a major role in the fusion of the virus with host cells membrane. The immunity system enables to produce antibody against HA2. The aim of this study is polyclonal antibody production against influenza HA2. Methods: This study was done in the Influenza Research Lab, Pasteur Institute of Iran, Tehran for one year from September 2013 to October 2014. In the present study, recombinant HA2 protein was produced in prokaryotic system and purified using Nickel affinity chromatography. The purified HA2 was mixed with Freund’s adjuvant (complete and incomplete and injected into two New Zealand white rabbits by intramuscularly and subcutaneously routes. Immunization was continued for several months with two weeks interval. Before each immunization, blood was drawn by venous puncture from the rabbit ear. Function of rabbit's sera was evaluated using radial immunodiffusion (RID in both forms, Single RID (SRID and Double RID (DRID. Finally, antiserum activity against HA2 was evaluated using western blotting as serological assay. Results: Sedimentary line and zone was observed in RID assays (SRID and DRID represent interaction between HA2 protein and anti- HA2 antibody. As well as, western blotting results was positive for HA2 protein. Therefore, these results showed that polyclonal antibody produced against HA2 protein can identify HA2 protein antigenic sites. Conclusion: These findings show that humoral immune responses have properly been stimulated in rabbits and these antibodies can identify HA2 protein and may be suitable for other serological methods.

Oceanographic station, temperature profiles, meteorological, and other data from XBT and bottle casts from NOAA Ship OREGON II as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1972-07-13 to 1972-08-08 (NODC Accession 7300271)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Oceanographic station, temperature profiles, meteorological, and other data were collected from bottle and XBT casts from NOAA Ship OREGON II from 13 July 1972 to 08...
Overview of the TIBER II design

International Nuclear Information System (INIS)

Henning, C.D.; Logan, B.G.

1987-01-01

The TIBER II Tokamak Ignition/Burn Experimental Reactor design is the result of efforts by numerous people and institutions, including many fusion laboratories, universities, and industries. This overview attempts to place the work in perspective. Major features of the design are compact size, low cost, and steady-state operation. These are achieved through plasma shaping and innovative features such as radiation tolerant magnets and optimized shielding. While TIBER II can operate in a pulsed mode, steady-state is preferred for nuclear testing. Current drive is achieved by a combination of lower hybrid and neutral beams. In addition to 10 MW of ECR is added for disruption control and current drive profiling
Los niños del Cauca II. Receptor de la vitamina D con secuencia normal en un foco de pacientes con raquitismo dependiente de la vitamina D, tipo II

Directory of Open Access Journals (Sweden)

Luis Fernando García-Ramirez

1993-12-01

Full Text Available El Raquitismo Dependiente de Vitamina D, tipo II (RDVD II, es una enfermedad de herencia autosómica recesiva que se caracteriza por resistencia generalizada a la 1,25-dihidroxivitamina D, originada en alteraciones que comprometen la función del receptor para la vitamina D (RVD. El clonaje y caracterización del cDNA del RVD ha permitido el estudio de su secuencia en pacientes con RDVD II y el hallazgo de diversas mutaciones puntuales que explican el cuadro clínico. En este estudio, hemos utilizado las técnicas de PCR y clonación, para secuenciar el RVD de dos pacientes pertenecientes a un foco descrito previamente en el departamento del Cauca (Colombia, cuyas manifestaciones clínicas y de laboratorio son compatibles con RDVD II. La secuencia del RVD de nuestros pacientes fue normal, sugiriendo una alteración a nivel postranscripcional o postraduccional relacionada con la función del receptor, en lo que podría corresponder a una nueva variante de resistencia a la vitamina D.
Sääste hoitakse üha enam valuutas / Piret Reiljan

Index Scriptorium Estoniae

Reiljan, Piret, 1983-

2007-01-01

Ilmunud ka: Delovõje Vedomosti 18. juuli lk. 3. Üha enam eraisikuid on oma tähtajalisi ja säästuhoiuseid paigutanud valuutasse, kuna välisraha puhul on valuutarisk väiksem. Kommenteerivad Mart Sõrg, Sven Meimer. Diagramm: Valuutahoiuste maht tõusuteel; Üle aasta kestvatest eraisikute hoiustest 75% valuutas. Tabel: Kroonihoius intressi poolest kuldne kesktee. Lisa: Number
Airborne gamma-ray spectrometer and magnetometer survey: Cameron A, Arizona, detail area. Volume II A. Final report

International Nuclear Information System (INIS)

1983-01-01

Volume II A contains appendices for: stacked profiles; geologic histograms; geochemical histograms; speed and altitude histograms; geologic statistical tables; geochemical statistical tables; magnetic and ancillary profiles; and test line data
Personality profiles in adults with attention deficit hyperactivity disorder (ADHD).

Science.gov (United States)

Perroud, Nader; Hasler, Roland; Golay, Nicolas; Zimmermann, Julien; Prada, Paco; Nicastro, Rosetta; Aubry, Jean-Michel; Ardu, Stefano; Herrmann, François R; Giannakopoulos, Panteleimon; Baud, Patrick

2016-06-14

Previous studies suggested that the presence of ADHD in children and young adolescents may affect the development of personality. Whether or not the persistence of ADHD in adult life is associated with distinct personality patterns is still matter for debate. To address this issue, we compared the profiles of the Temperament and Character Inventory (TCI) that assesses personality dimensions in 119 adults ADHD and 403 controls. ANCOVA were used to examine group differences (controls vs. ADHD and ADHD inattentive type vs. ADHD combined + hyperactive/impulsive types) in Temperaments and Characters. Partial correlation coefficients were used to assess correlation between TCI and expression and severity of symptoms of ADHD. High novelty seeking (NS), harm avoidance (HA) and self-transcendence (ST) scores as well as low self-directedness (SD) and cooperativeness (C) scores were associated with ADHD diagnosis. Low SD was the strongest personality trait associated with adult ADHD. Cases with the ADHD inattentive type showed higher HA and lower SD scores compared to the combined and hyperactive/impulsive types. High HA scores correlated with inattention symptoms whereas high NS and ST scores were related to hyperactive symptoms. Finally low SD and high NS were associated with increased ADHD severity. Distinct temperaments were associated with inattentive versus hyperactive/impulsive symptoms supporting the heterogeneous nature of the disorder.
Trichomonas vaginalis Metalloproteinase Induces mTOR Cleavage of SiHa Cells

Science.gov (United States)

Quan, Juan-Hua; Choi, In-Wook; Yang, Jung-Bo; Zhou, Wei; Cha, Guang-Ho; Zhou, Yu; Ryu, Jae-Sook

2014-01-01

Trichomonas vaginalis secretes a number of proteases which are suspected to be the cause of pathogenesis; however, little is understood how they manipulate host cells. The mammalian target of rapamycin (mTOR) regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription. We detected various types of metalloproteinases including GP63 protein from T. vaginalis trophozoites, and T. vaginalis GP63 metalloproteinase was confirmed by sequencing and western blot. When SiHa cells were stimulated with live T. vaginalis, T. vaginalis excretory-secretory products (ESP) or T. vaginalis lysate, live T. vaginalis and T. vaginalis ESP induced the mTOR cleavage in both time- and parasite load-dependent manner, but T. vaginalis lysate did not. Pretreatment of T. vaginalis with a metalloproteinase inhibitor, 1,10-phenanthroline, completely disappeared the mTOR cleavage in SiHa cells. Collectively, T. vaginalis metallopeptidase induces host cell mTOR cleavage, which may be related to survival of the parasite. PMID:25548410
EX0905 Water Column Summary Report and Profile Data Collection

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — A complete set of water column profile data and CTD Summary Report (if generated) generated by the Okeanos Explorer during EX0905: Mapping Field Trials II Mendocino...
Oceanographic Station, temperature profiles, and other data from CTD, XBT, and bottle casts from NOAA Ship DELAWARE II as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) from 1972-07-01 to 1972-08-13 (NODC Accession 7201299)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Oceanographic Station,temperature profiles, and other data were collected from CTD, XBT, and bottle casts from NOAA Ship DELAWARE II from 01 July 1972 to 13 August...
24 CFR 964.18 - HA role in activities under subparts B & C.

Science.gov (United States)

2010-04-01

... encourage the formation of joint HA management-resident committees to work on issues and planning. (9) The... elements of their partnership agreement and it shall be updated at least once every three (3) years. (11...
Turn-by-Turn Imaging of the Transverse Beam Profile in PEP-II

Energy Technology Data Exchange (ETDEWEB)

Fisher, Alan A.; Petree, Mark; /SLAC

2006-12-18

During injection or instability, the transverse profile of an individual bunch in a storage ring can change significantly in a few turns. However, most synchrotron-light imaging techniques are not designed for this time scale. We have developed a novel diagnostic that enhances the utility of a fast gated camera by adding, inexpensively, some features of a dual-axis streak camera, in order to watch the turn-by-turn evolution of the transverse profile, in both x and y. The beam's elliptical profile is reshaped using cylindrical lenses to form a tall and narrow ellipse--essentially the projection of the full ellipse onto one transverse axis. We do this projection twice, by splitting the beam into two paths at different heights, and rotating the ellipse by 90{sup o} on one path. A rapidly rotating mirror scans these vertical ''pencils'' of light horizontally across the photocathode of the camera, which is gated for 3 ns on every Nth ring turn. A single readout of the camera captures 100 images, looking like a stroboscopic photograph of a moving object. We have observed the capture of injected charge into a bunch and the rapid change of beam size at the onset of a fast instability.
Research and performance evaluation on an HA integrated acid system for sandstone acidizing

Directory of Open Access Journals (Sweden)

Liqiang Zhao

2018-03-01

Full Text Available When the conventional sandstone acidizing technologies are adopted, many slugs are needed in the injection of prepad fluid, treatment fluid and postpad fluid, and consequently the production and operation suffers inconveniences and difficulties. In view of this, a kind of HA integrated acid system which is mainly composed of organic polybasic acids (HA+HCl + HF and an efficient organic solvent was developed in this paper based on the idea of integrated acid replacing ''multiple steps'' and high efficiency and intensification. Via this HA integrated acid system, the complicated blockage in sandstone reservoirs can be removed effectively. Then, experiments were carried out on this system to evaluate its performance in terms of its retardance, organic blockage dissolution, chelating and precipitation inhibition. It is indicated that this new system can not only realize the acidizing of conventional integrated acid, but also present a good retarding performance by controlling H+ multi-stage ionization step by step and by forming silica acid-aluminum phosphonate film on the surface of clay minerals; that via this new HA integrated acid system, the organic blockage can be removed efficiently; and that it is wider in pH solution range than conventional APCs (aminopolycarboxyliates chelants, stronger in chelating capacity of Ca2+, Mg2+ and Fe3+ than conventional chelants (e.g. EDTA, NTA and DTPA, and better in precipitation inhibition on metal fluoride, fluosilicic acid alkali metal, fluoaluminic acid alkali metal and hydroxide than multi-hydrogen acid, fluoboric acid and mud acid systems. These research results provide a technical support for the plugging removal in high-temperature deep oil and gas reservoirs. Keywords: Organic polybasic acid, Integrated acid, Retardance, Chelating, Precipitation, Acidizing, Sandstone, Reservoir
National-Scale Changes in Soil Profile C and N in New Zealand Pastures are Determined by Land Use

Science.gov (United States)

Schipper, L. A.; Parfitt, R.; Ross, C.; Baisden, W. T.; Claydon, J.; Fraser, S.

2010-12-01

Grazed pasture is New Zealand’s predominant agricultural land-use and has been relatively recently developed from forest and native grasslands/shrub communities. From the 1850s onwards, land was cleared and exotic pastures established. Phosphorus fertilizer was increasingly used after 1950 which accelerated N fixation by clover. In the last two decades N fertilizers have been used, and grazing intensity has increased, thus affecting soil C and N. Re-sampling of 31 New Zealand soil profiles under grazed pasture measured surprisingly large losses of C and N over the last 2-3 decades (Schipper et al., 2007 Global Change Biology 13:1138-1144). These profiles were predominantly on the most intensively grazed flat land. We extended this re-sampling to 83 profiles (to 90 cm depth), to investigate whether changes in soil C and N stocks also occurred in less intensively managed pasture. Archived soils samples were analysed for total soil C and N alongside the newly collected samples. Intact cores were collected to determine bulk density through the profile. Over an average of 27 years, soils (0-30 cm) in flat dairy pastures significantly lost 0.73±0.16 Mg C ha-1y-1 and 57±16 kg N ha-1y-1 while we observed no change in soil C or N in flat pasture grazed by “dry stock” (e.g., sheep, beef), or in grazed tussock grasslands. Grazed hill country soils (0-30 cm) gained 0.52±0.18 Mg C ha-1y-1 and 66±18 kg N ha-1y-1. The losses of C and N were strongly correlated and C:N ratio has generally declined suggesting soils are becoming N saturated. Losses and gains also occurred in soil layers below 30 cm demonstrating that organic matter throughout the profile was responding to land use. The losses under dairying may be due to greater grazing pressure, fertilizer inputs and exports of C and N. There is evidence that grazing pressure reduces inputs of C below ground, reduces soil microbial C, and that dairy cow urine can mobilise C and N. Gains in hill country pastures may be due
Characterization of electrolytic HA/ZrO{sub 2} double layers coatings on Ti-6Al-4V implant alloy

Energy Technology Data Exchange (ETDEWEB)

Yen, S.K. [Department of Materials Engineering, National Chung Hsing University, Taichung 40227, Taiwan (China)]. E-mail: skyen@dragon.nchu.edu.tw; Chiou, S.H. [Graduate Institute of Veterinary Microbiology, National Chung Hsing University, Taichung 40227, Taiwan (China); Wu, S.J. [Department of Materials Engineering, National Chung Hsing University, Taichung 40227, Taiwan (China); Chang, C.C. [Department of Materials Engineering, National Chung Hsing University, Taichung 40227, Taiwan (China); Lin, S.P. [Department of Materials Engineering, National Chung Hsing University, Taichung 40227, Taiwan (China); Lin, C.M. [Department of Materials Engineering, National Chung Hsing University, Taichung 40227, Taiwan (China)

2006-01-15

Hydroxyapatite (HA) coating was proved having bioactive property and hence improving the bonding strength on bone tissue without inducing the growth of fiber tissue. However, the weak adhesion between HA and metal implants is still the major problem. In this study, a novel method of electrolytic HA/ZrO{sub 2} double layers coating was successfully conducted on F-136 Ti-6Al-4V implant alloy in ZrO{sub 2}(NO{sub 3}){sub 2} aqueous solution and subsequently in the mixed solution of Ca(NO{sub 3}){sub 2} and NH{sub 4}H{sub 2}PO{sub 4}. After annealing at 400 deg. C, 500 deg. C and 600 deg. C for 4 h in air, the coated specimens were evaluated by X-ray diffraction analyses, surface morphology observations, scratch tests, dynamic polarization tests, immersion tests and cell culture assays. In addition to corrosion resistance, the adhesion strength of electrolytic deposited HA on Ti alloy was dramatically improved from the critical scratch load 2 N to 32 N by adding the intermediate electrolytic deposition of ZrO{sub 2}, which showed the strong bonding effects between Ti alloy substrate and HA coating. Based on the cell morphology and cell proliferation data, HA/ZrO{sub 2} double layers coating revealed the better substrate for the adhesion and proliferation of osteoblasts than the others. It was also found that the crystallization of HA had positive effect on the proliferation of osteoblasts.
Role of Rhodobacter sphaeroides photosynthetic reaction center residue M214 in the composition, absorbance properties, and conformations of H(A) and B(A) cofactors.

Science.gov (United States)

Saer, Rafael G; Hardjasa, Amelia; Rosell, Federico I; Mauk, A Grant; Murphy, Michael E P; Beatty, J Thomas

2013-04-02

In the native reaction center (RC) of Rhodobacter sphaeroides, the side chain of (M)L214 projects orthogonally toward the plane and into the center of the A branch bacteriopheophytin (BPhe) macrocycle. The possibility that this side chain is responsible for the dechelation of the central Mg(2+) of bacteriochlorophyll (BChl) was investigated by replacement of (M)214 with residues possessing small, nonpolar side chains that can neither coordinate nor block access to the central metal ion. The (M)L214 side chain was also replaced with Cys, Gln, and Asn to evaluate further the requirements for assembly of the RC with BChl in the HA pocket. Photoheterotrophic growth studies showed no difference in growth rates of the (M)214 nonpolar mutants at a low light intensity, but the growth of the amide-containing mutants was impaired. The absorbance spectra of purified RCs indicated that although absorbance changes are associated with the nonpolar mutations, the nonpolar mutant RC pigment compositions are the same as in the wild-type protein. Crystal structures of the (M)L214G, (M)L214A, and (M)L214N mutants were determined (determined to 2.2-2.85 Å resolution), confirming the presence of BPhe in the HA pocket and revealing alternative conformations of the phytyl tail of the accessory BChl in the BA site of these nonpolar mutants. Our results demonstrate that (i) BChl is converted to BPhe in a manner independent of the aliphatic side chain length of nonpolar residues replacing (M)214, (ii) BChl replaces BPhe if residue (M)214 has an amide-bearing side chain, (iii) (M)214 side chains containing sulfur are not sufficient to bind BChl in the HA pocket, and (iv) the (M)214 side chain influences the conformation of the phytyl tail of the BA BChl.
PROFIL KESULITAN BELAJAR FISIKA POKOK BAHASAN KELISTRIKAN SISWA SMA DI KOTA SEMARANG

Directory of Open Access Journals (Sweden)

Ani Rusilowati

2012-01-01

Full Text Available Tujuan penelitian ini adalah menentukan profil kesulitan belajar Fisika, khususnya pokok bahasan Kelistrikan yang dialami oleh siswa SMA di kota Semarang. Sampel penelitian adalah siswa SMA kelas X di kota Semarang, diambil secara cluster, dari SMA negeri dan swasta peringkat I, II, dan III, sebanyak 214 siswa. Kesulitan belajar didiagnosis dengan lima pendekatan, yaitu tujuan pembelajaran, pengetahuan prasyarat, profil materi, miskonsepsi, dan pengetahuan terstruktur. Kesulitan belajar Kelistrikan antara lain disebabkan oleh rendahnya penguasaan konsep, lemahnya kemampuan matematis, dan kekurangmampuan mengkonversi satuan. Penyebab kesulitan belajar dalam pengetahuan terstruktur adalah rendahnya kemampuan: verbal, menggunakan skema, membuat strategi pemecahan masalah, dan membuat algoritma. Hasil penelitian menunjukkan bahwa kesulitan belajar Kelistrikan rata-rata terjadi pada sub pokok bahasan: Kuat Arus Listrik, Hukum Ohm, Hambatan Penghantar, Hukum Kirchof II, Energi & Daya Listrik, dan Transformator. Sebagian siswa masih mengalami miskonsepsi terhadap konsep Hukum Ohm dan Hambatan Penghantar. Bagi siswaÂ sekolah peringkat III mengalami kesulitan belajar di semua aspek dan materi Kelistrikan Kata kunci : profil, kesulitan belajar, kelistrikan
New dynamic NNORSY ozone profile climatology

Science.gov (United States)

Kaifel, A. K.; Felder, M.; Declercq, C.; Lambert, J.-C.

2012-01-01

Climatological ozone profile data are widely used as a-priori information for total ozone using DOAS type retrievals as well as for ozone profile retrieval using optimal estimation, for data assimilation or evaluation of 3-D chemistry-transport models and a lot of other applications in atmospheric sciences and remote sensing. For most applications it is important that the climatology represents not only long term mean values but also the links between ozone and dynamic input parameters. These dynamic input parameters should be easily accessible from auxiliary datasets or easily measureable, and obviously should have a high correlation with ozone. For ozone profile these parameters are mainly total ozone column and temperature profile data. This was the outcome of a user consultation carried out in the framework of developing a new, dynamic ozone profile climatology. The new ozone profile climatology is based on the Neural Network Ozone Retrieval System (NNORSY) widely used for ozone profile retrieval from UV and IR satellite sounder data. NNORSY allows implicit modelling of any non-linear correspondence between input parameters (predictors) and ozone profile target vector. This paper presents the approach, setup and validation of a new family of ozone profile climatologies with static as well as dynamic input parameters (total ozone and temperature profile). The neural network training relies on ozone profile measurement data of well known quality provided by ground based (ozonesondes) and satellite based (SAGE II, HALOE, and POAM-III) measurements over the years 1995-2007. In total, four different combinations (modes) for input parameters (date, geolocation, total ozone column and temperature profile) are available. The geophysical validation spans from pole to pole using independent ozonesonde, lidar and satellite data (ACE-FTS, AURA-MLS) for individual and time series comparisons as well as for analysing the vertical and meridian structure of different modes of
Clinical significance of changes of serum TBA, CG, HA levels in neonate with parenteral nutrition

International Nuclear Information System (INIS)

Huang Weiliang; Zhou Jiongying; Zhang Xiaoyi; Lv Weihua; Ma Yunbao; He Qizhi

2010-01-01

Objective: To study the clinical significance of changes of serum levels of TBA, CG, HA in neonate with parenteral nutrition. Methods: Serum total bile acid (TBA, with biochemistry) and CG, HA (with RIA) contents were measured in 52 neonates (full-term 32, preterm 20) with parenteral nutrition and 28 neonates (full-term 16, preterm 12) without parenteral nutrition (as controls). Results: Before parenteral nutrition,the serum TBA, CG and HA levels in full-term neonates were not significantly different from those in the controls (P>0.05). After parenteral nutrition,serum levels were significantly higher than those before parenteral nutrition (P<0.01). The levels in pre-term neonates were significantly higher after parenteral nutrition than those in full-term neonates (P<0.05). Conclusion: Long term parenteral nutrition might be harmful to hepatic and gall bladder function in neonates especially in premature ones. (authors)
v-Ha-ras oncogene insertion: A model for tumor progression of human small cell lung cancer

International Nuclear Information System (INIS)

Mabry, M.; Nakagawa, Toshitaro; Nelkin, B.D.; McDowell, E.; Gesell, M.; Eggleston, J.C.; Casero, R.A. Jr.; Baylin, S.B.

1988-01-01

Small cell lung cancer (SCLC) manifests a range of phenotypes in culture that may be important in understanding its relationship to non-SCLCs and to tumor progression events in patients. Most SCLC-derived cell lines, termed classic SCLC lines, have properties similar to SCLC tumors in patients. To delineate further the relationships between these phenotypes and the molecular events involved, the authors inserted the v-Ha-ras gene in SCLC cell lines with (biochemical variant) and without (classic) an amplified c-myc gene. These two SCLC subtypes had markedly different phenotypic responses to similar levels of expression of v-Ha-ras RNA. No biochemical or morphologic changes were observed in classic SCLC cells. In contrast, in biochemical variant SCLC cells, v-Ha-ras expression induced features typical of large cell undifferentiated lung carcinoma. Expression of v-Ha-ras in biochemical variant SCLC cells directly demonstrates that important transitions can occur between phenotypes of human lung cancer cells and that these may play a critical role in tumor progression events in patients. The finding provide a model system to study molecular events involved in tumor progression steps within a series of related tumor types
Molecular, Pathological, Radiological, and Immune Profiling of Non-brainstem Pediatric High-Grade Glioma from the HERBY Phase II Randomized Trial.

Science.gov (United States)

Mackay, Alan; Burford, Anna; Molinari, Valeria; Jones, David T W; Izquierdo, Elisa; Brouwer-Visser, Jurriaan; Giangaspero, Felice; Haberler, Christine; Pietsch, Torsten; Jacques, Thomas S; Figarella-Branger, Dominique; Rodriguez, Daniel; Morgan, Paul S; Raman, Pichai; Waanders, Angela J; Resnick, Adam C; Massimino, Maura; Garrè, Maria Luisa; Smith, Helen; Capper, David; Pfister, Stefan M; Würdinger, Thomas; Tam, Rachel; Garcia, Josep; Thakur, Meghna Das; Vassal, Gilles; Grill, Jacques; Jaspan, Tim; Varlet, Pascale; Jones, Chris

2018-05-14

The HERBY trial was a phase II open-label, randomized, multicenter trial evaluating bevacizumab (BEV) in addition to temozolomide/radiotherapy in patients with newly diagnosed non-brainstem high-grade glioma (HGG) between the ages of 3 and 18 years. We carried out comprehensive molecular analysis integrated with pathology, radiology, and immune profiling. In post-hoc subgroup analysis, hypermutator tumors (mismatch repair deficiency and somatic POLE/POLD1 mutations) and those biologically resembling pleomorphic xanthoastrocytoma ([PXA]-like, driven by BRAF_V600E or NF1 mutation) had significantly more CD8 + tumor-infiltrating lymphocytes, and longer survival with the addition of BEV. Histone H3 subgroups (hemispheric G34R/V and midline K27M) had a worse outcome and were immune cold. Future clinical trials will need to take into account the diversity represented by the term "HGG" in the pediatric population. Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

MHC class II molecules regulate growth in human T cells

DEFF Research Database (Denmark)

Nielsen, M; Odum, Niels; Bendtzen, K

1994-01-01

MHC-class-II-positive T cells are found in tissues involved in autoimmune disorders. Stimulation of class II molecules by monoclonal antibodies (mAbs) or bacterial superantigens induces protein tyrosine phosphorylation through activation of protein tyrosine kinases in T cells, and class II signals...... lines tested. Only one of three CD4+, CD45RAhigh, ROhigh T cells responded to class II costimulation. There was no correlation between T cell responsiveness to class II and the cytokine production profile of the T cell in question. Thus, T cell lines producing interferon (IFN)-gamma but not IL-4 (TH1...... modulate several T cell responses. Here, we studied further the role of class II molecules in the regulation of T cell growth. Costimulation of class II molecules by immobilized HLA-DR mAb significantly enhanced interleukin (IL)-2-supported T cell growth of the majority of CD4+, CD45RAlow, ROhigh T cell...
Abnormal MRI in a patient with 'headache with neurological deficits and CSF lymphocytosis (HaNDL)'.

Science.gov (United States)

Yilmaz, A; Kaleagasi, H; Dogu, O; Kara, E; Ozge, A

2010-05-01

A 27-year-old woman was admitted to the Emergency Department with right upper-extremity numbness and mild weakness followed by a bifrontal throbbing headache for 30 min, which was similar to a headache lasting for 12 h that had occurred 3 days ago. Laboratory tests were unremarkable except for cerebrospinal fluid (CSF) lymphocytic pleocytosis. On the following day, a headache episode with left hemiparesis and hemihypoaesthesia, left hemifield visio-spatial inattention, anosagnosia and confusion recurred. The headache was diagnosed as headache and neurological deficits with cerebrospinal fluid lymphocytosis (HaNDL) syndrome according to the criteria of the second edition of the International Classification of Headache Disorders. Simultaneously performed magnetic resonance imaging (MRI) revealed swelling of the grey matter, CSF enhancement in the sulci of the right temporal and occipital regions and hypoperfusion of the same brain regions. During the following 10 days two more similar episodes recurred and during the ensuing 12 months the patient remained headache free. Neuroimaging findings of the HaNDL syndrome are always thought as virtually normal. MRI abnormalities in our patient have not been reported in HaNDL syndrome previously, although they have been reported in hemiplegic migraine patients before. The findings in our case suggest that hemiplegic migraine and HaNDL syndrome may share a common pathophysiological pathway resulting in similar imaging findings and neurological symptoms.
ON RADIATION PRESSURE IN STATIC, DUSTY H II REGIONS

International Nuclear Information System (INIS)

Draine, B. T.

2011-01-01

Radiation pressure acting on gas and dust causes H II regions to have central densities that are lower than the density near the ionized boundary. H II regions in static equilibrium comprise a family of similarity solutions with three parameters: β, γ, and the product Q 0 n rms ; β characterizes the stellar spectrum, γ characterizes the dust/gas ratio, Q 0 is the stellar ionizing output (photons/s), and n rms is the rms density within the ionized region. Adopting standard values for β and γ, varying Q 0 n rms generates a one-parameter family of density profiles, ranging from nearly uniform density (small Q 0 n rms ) to shell-like (large Q 0 n rms ). When Q 0 n rms ∼> 10 52 cm -3 s -1 , dusty H II regions have conspicuous central cavities, even if no stellar wind is present. For given β, γ, and Q 0 n rms , a fourth quantity, which can be Q 0 , determines the overall size and density of the H II region. Examples of density and emissivity profiles are given. We show how quantities of interest-such as the peak-to-central emission measure ratio, the rms-to-mean density ratio, the edge-to-rms density ratio, and the fraction of the ionizing photons absorbed by the gas-depend on β, γ, and Q 0 n rms . For dusty H II regions, compression of the gas and dust into an ionized shell results in a substantial increase in the fraction of the stellar photons that actually ionize H (relative to a uniform-density H II region with the same dust/gas ratio and density n = n rms ). We discuss the extent to which radial drift of dust grains in H II regions can alter the dust-to-gas ratio. The applicability of these solutions to real H II regions is discussed.
Implant Angle Monitor System of MC3-II

International Nuclear Information System (INIS)

Sato, Fumiaki; Sano, Makoto; Nakaoka, Hiroaki; Fujii, Yoshito; Kudo, Tetuya; Nakanishi, Makoto; Koike, Masazumi; Fujino, Yasushi

2008-01-01

Precise implant angle control is required for the latest generation of ion implanters to meet further shrink semiconductor device requirements. Especially, the highest angle accuracy is required for Halo implant process of Logic devices. The Halo implant angle affects the device performance, because slight differences of beam divergence change the overlap profile towards the extension. Additionally, twist angle accuracy is demanded in case of channeling angle implant. Therefore monitoring beam angles and wafer twist angles is important. A new monitoring system for the MC3-II, SEN Corp.'s single wafer type medium current implanter has been developed. This paper describes the angle control performance and monitoring system of the MC3-II. For the twist angle control, we developed a wafer notch angle monitor. The system monitors the wafer notch image on the platen. And the notch angle variation is calculated by using image processing method. It is also able to adjust the notch angle according to the angle error. For the tilt angle control, we developed a vertical beam profile monitor. The monitor system can detect beam profile of vertical directions with horizontally scanning beam. It also measures beam angles of a tilt direction to a wafer. The system configuration and sample beam data are presented.
Inhibitory activity of pentacyano(isoniazid)ferrate(II), IQG-607, against promastigotes and amastigotes forms of Leishmania braziliensis

Science.gov (United States)

Amorim, Camila F.; Galina, Luiza; Carvalho, Natália B.; Sperotto, Nathalia D. M.; Pissinate, Kenia; Machado, Pablo; Campos, Maria M.; Basso, Luiz A.; Carvalho, Edgar M.; Santos, Diógenes Santiago

2017-01-01

M. tuberculosis and parasites of the genus Leishmania present the type II fatty acid biosynthesis system (FASII). The pentacyano(isoniazid)ferrate(II) compound, named IQG-607, inhibits the enzyme 2-trans-enoyl-ACP(CoA) reductase from M. tuberculosis, a key component in the FASII system. Here, we aimed to evaluate the inhibitory activity of IQG-607 against promastigote and amastigote forms of Leishmania (Viannia) braziliensis isolated from patients with different clinical forms of L. braziliensis infection, including cutaneous, mucosal and disseminated leishmaniasis. Importantly, IQG-607 inhibited the proliferation of three different isolates of L. braziliensis promastigotes associated with cutaneous, mucosal and disseminated leishmaniasis. The IC50 values for IQG-607 ranged from 32 to 75 μM, for these forms. Additionally, IQG-607 treatment decreased the proliferation of intracellular amastigotes in infected macrophages, after an analysis of the percentage of infected cells and the number of intracellular parasites/100 cells. IQG-607 reduced from 58% to 98% the proliferation of L. braziliensis from cutaneous, mucosal and disseminated strains. Moreover, IQG-607 was also evaluated regarding its potential toxic profile, by using different cell lines. Cell viability of the lineages Vero, HaCat and HepG2 was significantly reduced after incubation with concentrations of IQG-607 higher than 2 mM. Importantly, IQG-607, in a concentration of 1 mM, did not induce DNA damage in HepG2 cells, when compared to the untreated control group. Future studies will confirm the mechanism of action of IQG-607 against L. braziliensis. PMID:29281707
CRED Shallow CTD Profiles; Rota, Commonwealth of the Northern Mariana Islands; Cruise: HA1101_LEGIII, Data Date Range: 20110501-20110502. (NODC Accession 0107470)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
CRED Shallow CTD Profiles; Agrihan, Commonwealth of the Northern Mariana Islands; Cruise: HA1101_LEGII, Data Date Range: 20110420-20110422. (NODC Accession 0107470)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
CRED Shallow CTD Profiles; Saipan, Commonwealth of the Northern Mariana Islands; Cruise: HA1101_LEGII, Data Date Range: 20110407-20110426. (NODC Accession 0107470)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
CRED Shallow CTD Profiles; Pagan, Commonwealth of the Northern Mariana Islands; Cruise: HA1101_LEGII, Data Date Range: 20110411-20110413. (NODC Accession 0107470)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
CRED Shallow CTD Profiles; Maug, Commonwealth of the Northern Mariana Islands; Cruise: HA1101_LEGII, Data Date Range: 20110418-20110420. (NODC Accession 0107470)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
CRED Shallow CTD Profiles; Saipan, Commonwealth of the Northern Mariana Islands; Cruise: HA1101_LEGIII, Data Date Range: 20110430-20110430. (NODC Accession 0107470)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
CRED Shallow CTD Profiles; Tinian, Commonwealth of the Northern Mariana Islands; Cruise: HA1101_LEGIII, Data Date Range: 20110502-20110503. (NODC Accession 0107470)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
Airborne gamma-ray spectrometer and magnetometer survey: Monument Valley B, Utah, detail area. Volume II A. Final report

International Nuclear Information System (INIS)

1983-01-01

Volume II A contains appendices for: stacked profiles; geologic histograms; geochemical histograms; speed and altitude histograms; geologic statistical tables; geochemical statistical tables; magnetic and ancillary profiles; and test line data
Zonation of heme synthesis enzymes in mouse liver and their regulation by β-catenin and Ha-ras.

Science.gov (United States)

Braeuning, Albert; Schwarz, Michael

2010-11-01

Cytochrome P450 (CYP) hemoproteins play an important role in hepatic biotransformation. Recently, β-catenin and Ha-ras signaling have been identified as players controlling transcription of various CYP genes in mouse liver. The aim of the present study was to analyze the role of β-catenin and Ha-ras in the regulation of heme synthesis. Heme synthesis-related gene expression was analyzed in normal liver, in transgenic mice expressing activated β-catenin or Ha-ras, and in hepatomas. Regulation of the aminolevulinate dehydratase promoter was studied in vitro. Elevated expression of mRNAs and proteins involved in heme biosynthesis was linked to β-catenin activation in perivenous hepatocytes, in transgenic hepatocytes, and in hepatocellular tumors. Stimulation of the aminolevulinate dehydratase promoter by β-catenin was independent of the β-catenin/T-cell-specific transcription factor dimer. By contrast, activation of Ha-ras repressed heme synthesis-related gene expression. The present data suggest that β-catenin enhances the expression of both CYPs and heme synthesis-related genes, thus coordinating the availability of CYP apoprotein and its prosthetic group heme. The reciprocal regulation of heme synthesis by β-catenin and Ha-ras-dependent signaling supports our previous hypothesis that antagonistic action of these pathways plays a major role in the control of zonal gene expression in healthy mouse liver and aberrant expression patterns in hepatocellular tumors.
Processing of influenza HA protein in MDCK cells: components with different mobilities in polyacrylamide gel electrophoresis and their precursor-product relationships

International Nuclear Information System (INIS)

Sklyanskaya, E.I.; Rudneva, I.A.; Vovk, T.S.; Kaverin, N.V.

1980-01-01

In influenza virus-infected MDCK cells labelled with 14 C-chlorella hydrolysate or 35 S-methionine a virus-specific protein component is revealed migrating slightly faster than HA protein in polyacrylamide gel electrophoresis. Under chase conditions the component disappears either completely or partially, with a concomitant intensification of the HA band. The rate and extent of this transition are strain-dependent. Both the HA band and the faster moving component are not revealed if the cells are labelled in the presence of 20 mM of D-glucosamine. In primary cell cultures of chick embryos a single HA band with a mobility similar to that of the faster moving component in MDCK cells has been observed. It is suggested that the transition of the label from the faster moving component to the HA band reflects the final step of HA processing specific for MDCK cells. (author)
La gestion des ressources naturelles dans la commune de Hong Ha ...

International Development Research Centre (IDRC) Digital Library (Canada)

approvisionnement utilisée par l'armée nord-vietnamienne dans les années 1960 et 1970, la commune de Hong Ha — qui .... J'ai remboursé le prêt et utilisé les profits pour acheter de la nourriture et envoyer mes enfants à l'école. ».
The outer disks of early-type galaxies. I. Surface-brightness profiles of barred galaxies

NARCIS (Netherlands)

Erwin, Peter; Pohlen, Michael; Beckman, John E.

We present a study of 66 barred, early-type (S0-Sb) disk galaxies, focused on the disk surface brightness profile outside the bar region, with the aim of throwing light on the nature of Freeman type I and II profiles, their origins, and their possible relation to disk truncations. This paper
An overview af SAGE I and II ozone measurements

Science.gov (United States)

Mccormick, M. P.; Zawodny, J. M.; Veiga, R. E.; Larsen, J. C.; Wang, P. H.

1989-01-01

The stratospheric Aerosol and Gas Experiments (SAGE) I and II measure Mie, Rayleigh, and gaseous extinction profiles using the solar occultation technique. These global measurements yield ozone profiles with a vertical resolution of 1 km which have been routinely obtained for the periods from February 1979 to November 1981 (SAGE I) and October 1984 to the present (SAGE II). The long-term periodic behavior of the measured ozone is presented as well as case studies of the observed short-term spatial and temporal variability. A linear regression shows annual, semiannual, and quasi-biennial oscillation features at various altitudes and latitudes which, in general, agree with past work. Also, ozone, aerosol, and water vapor data are described for the Antarctic springtime, showing large variation relative to the vortex. Cross-sections in latitude and altitude and polar plots at various altitudes clearly delineate the ozone hole vertically and areally.
Rheological and structural characterization of HA/PVA-SbQ composites film-forming solutions and resulting films as affected by UV irradiation time.

Science.gov (United States)

Bai, Huiyu; Sun, Yunlong; Xu, Jing; Dong, Weifu; Liu, Xiaoya

2015-01-22

Hyaluronan (HA)/poly (vinyl alcohol) bearing styrylpyridinium groups (PVA-SbQ) composites film-forming solutions were prepared by a negatively charged HA and an oppositely charged PVA-SbQ. The rheological properties and structural characterization of HA/PVA-SbQ composites in aqueous solution were investigated. Zeta potential measurements and TEM were utilized to explore the formation of HA/PVA-SbQ complex micelles in aqueous solution. UV spectra and DLS experiments confirmed that the micelles are photo-crosslinkable. HA/PVA-SbQ composites films were prepared by a casting method. The microstructure and properties of the film were analyzed by SEM, optical transmittance, DSC, XRD and tensile testing. The crosslinked HA/PVA-SbQ composites films exhibited higher UV light shielding and visible light transparency and better mechanical and water vapor barrier properties as well as thermal stability than the uncrosslinked HA/PVA-SbQ composites films, indicating the formation of three-dimensional network structure. This work provided a good way for increasing the mechanical, thermal, water vapor barrier, and optical properties of HA materials for the packaging material. Copyright © 2014 Elsevier Ltd. All rights reserved.
Community-aware user profile enrichment in folksonomy.

Science.gov (United States)

Xie, Haoran; Li, Qing; Mao, Xudong; Li, Xiaodong; Cai, Yi; Rao, Yanghui

2014-10-01

In the era of big data, collaborative tagging (a.k.a. folksonomy) systems have proliferated as a consequence of the growth of Web 2.0 communities. Constructing user profiles from folksonomy systems is useful for many applications such as personalized search and recommender systems. The identification of latent user communities is one way to better understand and meet user needs. The behavior of users is highly influenced by the behavior of their neighbors or community members, and this can be utilized in constructing user profiles. However, conventional user profiling techniques often encounter data sparsity problems as data from a single user is insufficient to build a powerful profile. Hence, in this paper we propose a method of enriching user profiles based on latent user communities in folksonomy data. Specifically, the proposed approach contains four sub-processes: (i) tag-based user profiles are extracted from a folksonomy tripartite graph; (ii) a multi-faceted folksonomy graph is constructed by integrating tag and image affinity subgraphs with the folksonomy tripartite graph; (iii) random walk distance is used to unify various relationships and measure user similarities; (iv) a novel prototype-based clustering method based on user similarities is used to identify user communities, which are further used to enrich the extracted user profiles. To evaluate the proposed method, we conducted experiments using a public dataset, the results of which show that our approach outperforms previous ones in user profile enrichment. Copyright © 2014 Elsevier Ltd. All rights reserved.

Effect of gamma radiation and accelerated aging on the mechanical and thermal behavior of HDPE/HA nano-composites for bone tissue regeneration.

Science.gov (United States)

Alothman, Othman Y; Almajhdi, Fahad N; Fouad, H

2013-09-24

The replacement of hard tissues demands biocompatible and sometimes bioactive materials with properties similar to those of bone. Nano-composites made of biocompatible polymers and bioactive inorganic nano particles such as HDPE/HA have attracted attention as permanent bone substitutes due to their excellent mechanical properties and biocompatibility. The HDPE/HA nano-composite is prepared using melt blending at different HA loading ratios. For evaluation of the degradation by radiation, gamma rays of 35 kGy, and 70 kGy were used to irradiate the samples at room temperature in vacuum. The effects of accelerated ageing after gamma irradiation on morphological, mechanical and thermal properties of HDPE/HA nano-composites were measured. In Vitro test results showed that the HDPE and all HDPE/HA nano-composites do not exhibit any cytotoxicity to WISH cell line. The results also indicated that the tensile properties of HDPE/HA nano-composite increased with increasing the HA content except fracture strain decreased. The dynamic mechanical analysis (DMA) results showed that the storage and loss moduli increased with increasing the HA ratio and the testing frequency. Finally, it is remarked that all properties of HDPE/HA is dependent on the irradiation dose and accelerated aging. Based on the experimental results, it is found that the addition of 10%, 20% and 30% HA increases the HDPE stiffness by 23%, 44 and 59% respectively. At the same time, the G' increased from 2.25E11 MPa for neat HDPE to 4.7E11 MPa when 30% HA was added to the polymer matrix. Also, significant improvements in these properties have been observed due to irradiation. Finally, the overall properties of HDPE and its nano-composite properties significantly decreased due to aging and should be taken into consideration in the design of bone substitutes. It is attributed that the developed HDPE/HA nano-composites could be a good alternative material for bone tissue regeneration due to their acceptable
Programming Algorithms of load balancing with HA-Proxy in HTTP services

Directory of Open Access Journals (Sweden)

José Teodoro Mejía Viteri

2018-02-01

Full Text Available The access to the public and private services through the web gains daily protagonism, and sometimes they must support amounts of requests that a team can not process, so there are solutions that use algorithms that allow to distribute the load of requests of a web application in several equipment; the objective of this work is to perform an analysis of load balancing scheduling algorithms through the HA-Proxy tool, and deliver an instrument that identifies the load distribution algorithm to be used and the technological infrastructure, to largely cover implementation. The information used for this work is based on a bibliographic analysis, eld study and implementation of the different load balancing algorithms in equipment, where the distribution and its performance will be analyzed. The incorporation of this technology to the management of services on the web, improves availability, helps business continuity and through the different forms of distribution of the requests of the algorithms that can be implemented in HA-Proxy to provide those responsible for information technology systems with a view of their advantages and disadvantages.
Polycyclic Aromatic Hydrocarbon Emission in Spitzer /IRS Maps. II. A Direct Link between Band Profiles and the Radiation Field Strength

Energy Technology Data Exchange (ETDEWEB)

Stock, D. J.; Peeters, E., E-mail: dstock84@gmail.com [Department of Physics and Astronomy, University of Western Ontario, London, ON, N6A 3K7 (Canada)

2017-03-10

We decompose the observed 7.7 μ m polycyclic aromatic hydrocarbon (PAH) emission complexes in a large sample of over 7000 mid-infrared spectra of the interstellar medium using spectral cubes observed with the Spitzer /IRS-SL instrument. In order to fit the 7.7 μ m PAH emission complex we invoke four Gaussian components, which are found to be very stable in terms of their peak positions and widths across all of our spectra, and subsequently define a decomposition with fixed parameters, which gives an acceptable fit for all the spectra. We see a strong environmental dependence on the interrelationships between our band fluxes—in the H ii regions all four components are intercorrelated, while in the reflection nebulae (RNs) the inner and outer pairs of bands correlate in the same manner as previously seen for NGC 2023. We show that this effect arises because the maps of RNs are dominated by emission from strongly irradiated photodissociation regions, while the much larger maps of H ii regions are dominated by emission from regions much more distant from the exciting stars, leading to subtly different spectral behavior. Further investigation of this dichotomy reveals that the ratio of two of these components (centered at 7.6 and 7.8 μ m) is linearly related to the UV-field intensity (log G {sub 0}). We find that this relationship does not hold for sources consisting of circumstellar material, which are known to have variable 7.7 μ m spectral profiles.
Single-dose monomeric HA subunit vaccine generates full protection from influenza challenge

CSIR Research Space (South Africa)

Mallajosyula, JK

2014-03-01

Full Text Available 50% survival, or 100% survival with adjuvant, compared with 10% survival after vaccination with a commercially available H 1 N 1 vaccine. TMV-HA is an effective dose-sparing influenza vaccine, using a single-step process to rapidly generate large...
Groteskno Haškových cestovních povídek

Czech Academy of Sciences Publication Activity Database

Hrbata, Zdeněk

2014-01-01

Roč. 61, č. 3 (2014), s. 181-198 ISSN 0037-6973 R&D Projects: GA ČR GA13-29985S Institutional support: RVO:68378068 Keywords : travel ogues * grotesqueness * parody * Hašek, Jaroslav Subject RIV: AJ - Letters, Mass-media, Audiovision
Molecular analysis of genetic diversity in elite II synthetic hexaploid ...

African Journals Online (AJOL)

STORAGESEVER

2009-07-20

Jul 20, 2009 ... The presence of sufficient genetic diversity in the germplam is an important ..... Figure 1. PCR amplification profile of Elite-II SH Wheat using the primer OPG-2. .... genetic relationships among cowpea breeding lines and local.
Auditory profiling and hearing-aid satisfaction in hearing-aid candidates

DEFF Research Database (Denmark)

Thorup, Nicoline; Santurette, Sébastien; Jørgensen, Søren

2016-01-01

by default. This study aimed at identifying clinically relevant tests that may serve as an informative addition to the audiogram and which may relate more directly to HA satisfaction than the audiogram does. METHODS: A total of 29 HI and 26 normal-hearing listeners performed tests of spectral and temporal...... their audiogram. Measures of temporal resolution or speech perception in both stationary and fluctuating noise could be relevant measures to consider in an extended auditory profile. FUNDING: The study was supported by Grosserer L.F. Foghts Fond. TRIAL REGISTRATION: The protocol was approved by the Science Ethics...
Comparison between PCL/hydroxyapatite (HA) and PCL/halloysite nanotube (HNT) composite scaffolds prepared by co-extrusion and gas foaming.

Science.gov (United States)

Jing, Xin; Mi, Hao-Yang; Turng, Lih-Sheng

2017-03-01

In this work, three-dimensional poly(caprolactone) (PCL) tissue engineering scaffolds were prepared by co-extrusion and gas foaming. Biocompatible hydroxyapatite (HA) and halloysite nanotubes (HNT) were added to the polymer matrix to enhance the mechanical properties and bioactivity of the composite scaffolds. The effects of HA and HNT on the rheological behavior, microstructure, and mechanical properties of the composite scaffolds were systematically compared. It was found that the HNT improved viscosity more significantly than HA, and reduced the pore size of scaffolds, while the mechanical performance of PCL/HNT scaffolds was higher than PCL/HA scaffolds with the same filler content. Human mesenchymal stem cells (hMSCs) were used as the cell model to compare the biological properties of two composite scaffolds. The results demonstrated that cells could survive on all scaffolds, and showed a more flourishing living state on the composite scaffolds. The cell differentiation for 5% HA and 1% HNT scaffolds were significantly higher than other scaffolds, while the differentiation of 5% HNT scaffolds was lower than that of 1% HNT scaffolds mainly because of the reduced pore size and pore interconnectivity. Therefore, this study suggested that, with proper filler content and control of microstructure through processing, HNT could be a suitable substitute for HA for bone tissue engineering to reduce the cost and improve mechanical performance. Copyright © 2016. Published by Elsevier B.V.
Elemental profiling of Cabernet Sauvignon grapes as a function of geospatial variability in a Napa Valley vineyard

Science.gov (United States)

Carvalho, Angela; Hopfer, Helene; Nelson, Jenny; Ebeler, Sue; Jenkins, Christopher; Plant, Richard; Smart, David

2015-04-01

A primary tenant of the concept of geoscience and wine is that elemental composition of soils may be reflected in the elemental profile of fruit and discerned in the organoleptic assessment of wine. The extremely varied soil composition at the vineyard level in the Napa Valley region of California provides an ideal setting to study elemental pattern correlations between grape berries and soil samples. In the Napa Valley Cabernet Sauvignon is a wine grape variety of substantial economic value. Elemental profiling of Cabernet Sauvignon grapes in function of origin will provide a better understanding of the relationship between elemental accumulation in berries and soil element composition. The aim of this study was to explore the geospatial variability of elemental patterns in Cabernet Sauvignon grapes with respect to the soil elemental profiles at thirty-six geo-referenced vines in a 4 ha vineyard. Sixty-eight elements were determined via inductively coupled-plasma mass spectrometry (ICP-MS); this allowed for elemental profiling of both soil and berries at each sampling site. It was found that for the soil samples twenty-two elements contributed to a significant difference between sampling points, and thirty for the berries. Application of principal components analysis (PCA) showed that soil and berry elemental composition varied as a function of location in the vineyard. For the soil PCA, rare earth metals such as Dy, Ho, Ce, Er, Yb and Tm were driving separation towards the southern section of the vineyard while K, Ga, V, Al, Mg and P were correlated with the northern section. In the berry samples the Lanthanides, Gd, Pr, Yb, Dy, Er and Ho, also showed a higher influence in driving separation towards the southern section while Sr, Mo, Ba, Mg, P, K, Cd, Cu, B, Rb and Ti characterized the elemental profile of the northern part of the block. These findings showed that the rare earth metals, in particular Yb, Dy, Er and Ho, were the most distinguishing elemental
Metallpitsid Tallinna Püha Johannese hospidali (Jaani seegi) kalmistu haualeidudes / Sari Marjut Rainne

Index Scriptorium Estoniae

Rainne, Sari Marjut

2010-01-01

Tallinna Püha Johannese hospidali (Jaani seegi) kalmistualal toimunud arheoloogilistelt kaevamistelt leitud metallpitsidest. Ülevaade põhiliselt 18. sajandist pärit peakatete külge kinnitatud pitside materjaliuuringu tulemustest ja konserveerimisest
Crystal Structure of the Receptor-Binding Domain of Botulinum Neurotoxin Type HA, Also Known as Type FA or H.

Science.gov (United States)

Yao, Guorui; Lam, Kwok-Ho; Perry, Kay; Weisemann, Jasmin; Rummel, Andreas; Jin, Rongsheng

2017-03-08

Botulinum neurotoxins (BoNTs), which have been exploited as cosmetics and muscle-disorder treatment medicines for decades, are well known for their extreme neurotoxicity to humans. They pose a potential bioterrorism threat because they cause botulism, a flaccid muscular paralysis-associated disease that requires immediate antitoxin treatment and intensive care over a long period of time. In addition to the existing seven established BoNT serotypes (BoNT/A-G), a new mosaic toxin type termed BoNT/HA (aka type FA or H) was reported recently. Sequence analyses indicate that the receptor-binding domain (H C ) of BoNT/HA is ~84% identical to that of BoNT/A1. However, BoNT/HA responds differently to some potent BoNT/A-neutralizing antibodies (e.g., CR2) that target the H C . Therefore, it raises a serious concern as to whether BoNT/HA poses a new threat to our biosecurity. In this study, we report the first high-resolution crystal structure of BoNT/HA-H C at 1.8 Å. Sequence and structure analyses reveal that BoNT/HA and BoNT/A1 are different regarding their binding to cell-surface receptors including both polysialoganglioside (PSG) and synaptic vesicle glycoprotein 2 (SV2). Furthermore, the new structure also provides explanations for the ~540-fold decreased affinity of antibody CR2 towards BoNT/HA compared to BoNT/A1. Taken together, these new findings advance our understanding of the structure and function of this newly identified toxin at the molecular level, and pave the way for the future development of more effective countermeasures.
Crystal Structure of the Receptor-Binding Domain of Botulinum Neurotoxin Type HA, Also Known as Type FA or H

Directory of Open Access Journals (Sweden)

Guorui Yao

2017-03-01

Full Text Available Botulinum neurotoxins (BoNTs, which have been exploited as cosmetics and muscle-disorder treatment medicines for decades, are well known for their extreme neurotoxicity to humans. They pose a potential bioterrorism threat because they cause botulism, a flaccid muscular paralysis-associated disease that requires immediate antitoxin treatment and intensive care over a long period of time. In addition to the existing seven established BoNT serotypes (BoNT/A–G, a new mosaic toxin type termed BoNT/HA (aka type FA or H was reported recently. Sequence analyses indicate that the receptor-binding domain (HC of BoNT/HA is ~84% identical to that of BoNT/A1. However, BoNT/HA responds differently to some potent BoNT/A-neutralizing antibodies (e.g., CR2 that target the HC. Therefore, it raises a serious concern as to whether BoNT/HA poses a new threat to our biosecurity. In this study, we report the first high-resolution crystal structure of BoNT/HA-HC at 1.8 Å. Sequence and structure analyses reveal that BoNT/HA and BoNT/A1 are different regarding their binding to cell-surface receptors including both polysialoganglioside (PSG and synaptic vesicle glycoprotein 2 (SV2. Furthermore, the new structure also provides explanations for the ~540-fold decreased affinity of antibody CR2 towards BoNT/HA compared to BoNT/A1. Taken together, these new findings advance our understanding of the structure and function of this newly identified toxin at the molecular level, and pave the way for the future development of more effective countermeasures.
Crystal Structure of the Receptor-Binding Domain of Botulinum Neurotoxin Type HA, Also Known as Type FA or H

Energy Technology Data Exchange (ETDEWEB)

Yao, Guorui; Lam, Kwok-ho; Perry, Kay; Weisemann, Jasmin; Rummel, Andreas; Jin, Rongsheng (Cornell); (Dusseldorf); (UCI)

2017-03-01

Botulinum neurotoxins (BoNTs), which have been exploited as cosmetics and muscle-disorder treatment medicines for decades, are well known for their extreme neurotoxicity to humans. They pose a potential bioterrorism threat because they cause botulism, a flaccid muscular paralysis-associated disease that requires immediate antitoxin treatment and intensive care over a long period of time. In addition to the existing seven established BoNT serotypes (BoNT/A–G), a new mosaic toxin type termed BoNT/HA (aka type FA or H) was reported recently. Sequence analyses indicate that the receptor-binding domain (HC) of BoNT/HA is ~84% identical to that of BoNT/A1. However, BoNT/HA responds differently to some potent BoNT/A-neutralizing antibodies (e.g., CR2) that target the HC. Therefore, it raises a serious concern as to whether BoNT/HA poses a new threat to our biosecurity. In this study, we report the first high-resolution crystal structure of BoNT/HA-HC at 1.8 Å. Sequence and structure analyses reveal that BoNT/HA and BoNT/A1 are different regarding their binding to cell-surface receptors including both polysialoganglioside (PSG) and synaptic vesicle glycoprotein 2 (SV2). Furthermore, the new structure also provides explanations for the ~540-fold decreased affinity of antibody CR2 towards BoNT/HA compared to BoNT/A1. Taken together, these new findings advance our understanding of the structure and function of this newly identified toxin at the molecular level, and pave the way for the future development of more effective countermeasures
Dissolved inorganic carbon, pH, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the Ryofu Maru II in the North Pacific Ocean from 2005-06-15 to 2005-07-04 (NODC Accession 0109905)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0109905 includes biological, chemical, discrete sample, physical and profile data collected from Ryofu Maru II in the North Pacific Ocean from...
Early Intervention in Skeletal Class II and dental Class II division I malocclusion

Directory of Open Access Journals (Sweden)

Zeeshan Iqbal Bhat

2013-01-01

Full Text Available A Class II malocclusion may occur as a result of mandibular deficiency, maxillary excess, or a combination of both. However, the most common finding is mandibular skeletal retrusion. The use of functional jaw orthopedics, at the right time during growth, can ultimately result in malocclusion patients achieving an excellent functional occlusion, a broad beautiful smile, a full face with a beautiful jaw line, and profile. Functional jaw orthopedic (FJO appliances are designed to encourage adaptive skeletal growth by maintaining the mandible in a corrected forward position. The activator developed by Andresen is one of the most widely used for this purpose. A 12-year-old boy with skeletal Class II malocclusion and dental Class II div I malocclusion, a low mandibular plane angle was treated with growth modulation using an activator followed by molar distalization using fixed orthodontics for detailing of the occlusion. The major effects of the activator treatment in this case have been due to increase in condylar growth and also an increase in mandibular base length. Further, non-extraction fixed orthodontic treatment for proper interdigitation of the dentition also helped to maintain the stability of the satisfactory results achieved.
The sunflower transcription factor HaHB11 confers tolerance to water deficit and salinity to transgenic Arabidopsis and alfalfa plants.

Science.gov (United States)

Cabello, Julieta V; Giacomelli, Jorge I; Gómez, María C; Chan, Raquel L

2017-09-10

Homeodomain-leucine zipper (HD-Zip) transcription factors are unique to the plant kingdom; members of subfamily I are known to be involved in abiotic stress responses. HaHB11 belongs to this subfamily and it was previously shown that it is able to confer improved yield and tolerance to flooding via a quiescent strategy. Here we show that HaHB11 expression is induced by ABA, NaCl and water deficit in sunflower seedlings and leaves. Arabidopsis transgenic plants expressing HaHB11, controlled either by its own promoter or by the constitutive 35S CaMV, presented rolled leaves and longer roots than WT when grown under standard conditions. In addition, these plants showed wider stems and more vascular bundles. To deal with drought, HaHB11 transgenic plants closed their stomata faster and lost less water than controls, triggering an enhanced tolerance to such stress condition and also to salinity stress. Concomitantly, ABA-synthesis and sensing related genes were differentially regulated in HaHB11 transgenic plants. Either under long-term salinity stress or mild drought stress, HaHB11 transgenic plants did not exhibit yield penalties. Moreover, alfalfa transgenic plants were generated which also showed enhanced drought tolerance. Altogether, the results indicated that HaHB11 was able to confer drought and salinity tolerance via a complex mechanism which involves morphological, physiological and molecular changes. Copyright © 2016 Elsevier B.V. All rights reserved.
Forced convection heat transfer in He II

International Nuclear Information System (INIS)

Kashani, A.

1986-01-01

An investigation of forced convection heat transfer in He II is conducted. The study includes both experimental and theoretical treatments of the problem. The experiment consists of a hydraulic pump and a copper flow tube, 3 mm in ID and 2m long. The system allows measurements of one-dimensional heat and mass transfer in He II. The heat transfer experiments are performed by applying heat at the midpoint along the length of the flow tube. Two modes of heat input are employed, i.e., step function heat input and square pulse heat input. The heat transfer results are discussed in terms of temperature distribution in the tube. The experimental temperature profiles are compared with numerical solutions of an analytical model developed from the He II energy equation. The bath temperature is set at three different values of 1.65, 1.80, and 1.95 K. The He II flow velocity is varied up to 90 cm/s. Pressure is monitored at each end of the flow tube, and the He II pressure drop is obtained for different flow velocities. Results indicate that He II heat transfer by forced convention is considerably higher than that by internal convection. The theoretical model is in close agreement with the experiment. He II pressure drop and friction factor are very similar to those of an ordinary fluid
Synthesis and Characterization of Hydroxyapatite-Collagen-Chitosan (HA/Col/Chi) Composite Coated on Ti6Al4V

Science.gov (United States)

Charlena; Bikharudin, Ahmad; Wahyudi, Setyanto Tri

2018-01-01

HA-collagen-chitosan (HA/col/chi) composite is developed to increase bioactivity adhesiveness between the metal and the material composite and to improve corrosion resistance. The Ti6Al4V alloy was coated by soaking in HA/col/chi composite at room temperature and then allowed to stand for 5, 6, and 7 days. Diffraction pattern analysis of the coated Ti6Al4V alloy showed that the dominant phase were HA and Ti6Al4V alloy. Corrosion resistance test in media by using 0.9% NaCl showed the corrosion rate at the level of 0.3567 mpy, which was better than that of the uncoated Ti6Al4V alloy (0.4152 mpy). In vitro cytocompatibility assay on endothelial cell of calf pulmonary artery endothelium (CPAE) (ATCC-CCL 209) showed there was no toxicity in the cell culture with the percent inhibition of 33.33% after 72 hours of incubation.
Profiling first-year students in STEM programs based on autonomous motivation and academic self-concept and relationship with academic achievement.

Science.gov (United States)

Van Soom, Carolien; Donche, Vincent

2014-01-01

The low success rate of first-year college students in Science, Technology, Engineering, and Mathematics (STEM) programs has spurred many academic achievement studies in which explanatory factors are studied. In this study, we investigated from a person-oriented perspective whether different motivational and academic self-concept profiles could be discerned between male and female first-year college students in STEM and whether differences in early academic achievement were associated with these student groups. Data on autonomous motivation, academic self-concept, and early academic achievement of 1,400 first-year STEM college students were collected. Cluster analyses were used to distinguish motivational profiles based on the relative levels of autonomous motivation and academic self-concept for male and female students. Differences in early academic achievement of the various profiles were studied by means of ANCOVA. Four different motivational profiles were discerned based on the dimensions of autonomous motivation (A) and academic self-concept (S): students scoring high and respectively low on both dimensions (HA-HS or LA-LS), and students scoring high on one dimension and low on the other (HA-LS or LA-HS). Also gender differences were found in this study: male students with high levels of academic self-concept and autonomous motivation had higher academic achievement compared to male students with low levels on both motivational dimensions. For female students, motivational profiles were not associated with academic achievement. The findings partially confirm the internal and external validity of the motivational theories underpinning this study and extend the present insights on identifying subgroup(s) of at risk students in contemporary STEM programs at university level.
Profiling First-Year Students in STEM Programs Based on Autonomous Motivation and Academic Self-Concept and Relationship with Academic Achievement

Science.gov (United States)

Van Soom, Carolien; Donche, Vincent

2014-01-01

The low success rate of first-year college students in Science, Technology, Engineering, and Mathematics (STEM) programs has spurred many academic achievement studies in which explanatory factors are studied. In this study, we investigated from a person-oriented perspective whether different motivational and academic self-concept profiles could be discerned between male and female first-year college students in STEM and whether differences in early academic achievement were associated with these student groups. Data on autonomous motivation, academic self-concept, and early academic achievement of 1,400 first-year STEM college students were collected. Cluster analyses were used to distinguish motivational profiles based on the relative levels of autonomous motivation and academic self-concept for male and female students. Differences in early academic achievement of the various profiles were studied by means of ANCOVA. Four different motivational profiles were discerned based on the dimensions of autonomous motivation (A) and academic self-concept (S): students scoring high and respectively low on both dimensions (HA-HS or LA-LS), and students scoring high on one dimension and low on the other (HA-LS or LA-HS). Also gender differences were found in this study: male students with high levels of academic self-concept and autonomous motivation had higher academic achievement compared to male students with low levels on both motivational dimensions. For female students, motivational profiles were not associated with academic achievement. The findings partially confirm the internal and external validity of the motivational theories underpinning this study and extend the present insights on identifying subgroup(s) of at risk students in contemporary STEM programs at university level. PMID:25390942

Oceanographic station, temperature profile, meteorological, and other data from CTD and XBT casts from NOAA Ship DELAWARE II and other platforms as part of the Marine Resources Monitoring, Assessment and Prediction (MARMAP) project from 1980-06-25 to 1983-08-04 (NODC Accession 8300119)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Oceanographic station, temperature profile, meteorological, and other data were collected from CTD and XBT casts from NOAA Ship DELAWARE II and other platforms from...
Wear Behavior of Plasma Spray Deposited and Post Heat-Treated Hydroxyapatite (HA)-Based Composite Coating on Titanium Alloy (Ti-6Al-4V) Substrate

Science.gov (United States)

Kumari, Renu; Majumdar, Jyotsna Dutta

2018-04-01

The present study concerns a detailed evaluation of wear resistance property of plasma spray deposited composite hydroxyapatite (HA)-based (HA-50 wt pct TiO2 and HA-10 wt pct ZrO2) bioactive coatings developed on Ti-6Al-4V substrate and studying the effect of heat treatment on it. Heat treatment of plasma spray deposited samples has been carried out at 650 °C for 2 hours (for HA-50 wt pct TiO2 coating) and at 750 °C for 2 hours (for HA-10 wt pct ZrO2 coating). There is significant deterioration in wear resistance for HA-50 wt pctTiO2 coating and a marginal deterioration in wear resistance for HA-10 wt pct ZrO2 coating in as-sprayed state (as compared to as-received Ti-6Al-4V) which is, however, improved after heat treatment. The coefficient of friction is marginally increased for both HA-50 wt pct TiO2 and HA-10 wt pct ZrO2 coatings in as-sprayed condition as compared to Ti-6Al-4V substrate. However, coefficient of friction is decreased for both HA-50 wt pct TiO2 and HA-10 wt pct ZrO2 coatings after heat-treated condition as compared to Ti-6Al-4V substrate. The maximum improvement in wear resistance property is, however, observed for HA-10 wt pct ZrO2 sample after heat treatment. The mechanism of wear has been investigated.
Recombinant HA1 produced in E. coli forms functional oligomers and generates strain-specific SRID potency antibodies for pandemic influenza vaccines.

Science.gov (United States)

Khurana, Surender; Larkin, Christopher; Verma, Swati; Joshi, Manju B; Fontana, Juan; Steven, Alasdair C; King, Lisa R; Manischewitz, Jody; McCormick, William; Gupta, Rajesh K; Golding, Hana

2011-08-05

Vaccine production and initiation of mass vaccination is a key factor in rapid response to new influenza pandemic. During the 2009-2010 H1N1 pandemic, several bottlenecks were identified, including the delayed availability of vaccine potency reagents. Currently, antisera for the single-radial immunodiffusion (SRID) potency assay are generated in sheep immunized repeatedly with HA released and purified after bromelain-treatment of influenza virus grown in eggs. This approach was a major bottleneck for pandemic H1N1 (H1N1pdm09) potency reagent development in 2009. Alternative approaches are needed to make HA immunogens for generation of SRID reagents in the shortest possible time. In this study, we found that properly folded recombinant HA1 globular domain (rHA1) from several type A viruses including H1N1pdm09 and two H5N1 viruses could be produced efficiently using a bacterial expression system and subsequent purification. The rHA1 proteins were shown to form functional oligomers of trimers, similar to virus derived HA, and elicited high titer of neutralizing antibodies in rabbits and sheep. Importantly, the immune sera formed precipitation rings with reference antigens in the SRID assay in a dose-dependent manner. The HA contents in multiple H1N1 vaccine products from different manufacturers (and in several lots) as determined with the rHA1-generated sheep sera were similar to the values obtained with a traditionally generated sheep serum from NIBSC. We conclude that bacterially expressed recombinant HA1 proteins can be produced rapidly and used to generate SRID potency reagents shortly after new influenza strains with pandemic potential are identified. Published by Elsevier Ltd.
Green Chemistry; Sviluppo sostenibile. L'industria ha bisogno del contributo di tutti

Energy Technology Data Exchange (ETDEWEB)

Ingallina, P. [EniTecnologie SpA, San Donato Milanese, MI (Italy)

2001-02-01

Everyone acknowledges that chemistry is a key science in order to study and solve the problems of the environment: a successful arranging technological progress with environment protection is one of the main challenge of the next millennium. The Green Chemistry (or Sustainable Chemistry) represents the specific contribution that chemists can supply for an environmentally compatible development. [Italian] Fabrizio d'Adda, attualmente Presidente di EniChem e membro di Cefic (European Chemical Industry Council), ha aperto il seminario {sup T}he Greening of Chemistry{sup (}EniTecnologie - 31 Ottobre 2000) con un breve discorso. Ha espresso parole di fiducia riguardo al futuro dell'industria chimica, l'unica a suo giudizio, in grado di avviare un nuovo modello di sviluppo nel rispetto delle implicazioni ambientali, sociali ed economiche.
NBI Calculations for the TJ-II Experimental discharges

International Nuclear Information System (INIS)

Guasp, J.; Fuentes, C.; Liniers, M.

2004-01-01

Calculations for NBI losses, absorption and power deposition radial profiles, corresponding to the experimental TJ-II campaigns 2003-2004, have been fitted to simple functionals in order to allow a fast approximative evaluation for any given density. The average difference between the calculations for the individual discharges using the experimental density and temperature radial profiles and the fit predictions are between 10 and 15% and the behaviour with density is the expected one: nonotonic decrease of shine through losses and increase of absorption with incipient saturation for high densities. The fast ion birth radial profile narrows initially at low densities but later starts to widen, although, for the average line density range analysed (0.51 a 4.1x10''13 cm''-3), never are wide enough to induce an increase of direct orbit losses neither to produce hollow radial profiles. The power absorption radial profile widens nonotonically. There exist Fortran subroutines, available at the three CIEMAT computers, allowing the fast approximative evaluation of all these values. (Author) 8 refs
NBI Calculations for the TJ-II Experimental Discharges

International Nuclear Information System (INIS)

Guasp, J.; Fuentes, C.; Liniers, M.

2005-01-01

The density and electron temperature radial profiles, corresponding to the experimental TJ-II campaigns 2003-2004, with NBI, have been fitted to simple functionals in order to allow a fast approximative evaluation for any given density and injected power... The fits have been calculated, separately, for the four possibilities: ECRH and NBI Phases as well as On and Off Axis ECRH injection. The average difference between the experimental profiles for the individual discharges and the fit predictions are around 8% for the density and 10% for the temperature. The behaviour of the predicted profiles with average line density and injected power has been analysed. The central electron temperature decreases monotonically with increasing density and the ECRH phase On Axis central value is clearly higher than the Off axis one. The radial density profiles narrow with increasing density and the NBI On axis case is clearly wider than de Off one. The electron temperature profile widens slightly with increasing density and the width of the On Axix case is lesser than for the Off case in all phases. There exist Fortran subroutines, available at the three CIEMAT computers, allowing the fast approximative evaluation of all these profiles. (Author) 8 refs
The renal metallothionein expression profile is altered in human lupus nephritis

DEFF Research Database (Denmark)

Faurschou, Mikkel; Penkowa, Milena; Andersen, Claus Bøgelund

2008-01-01

of standard statistical methods. RESULTS: Proximal tubules displaying epithelial cell MT-I+II depletion in combination with luminal MT-I+II expression were observed in 31 out of 37 of the lupus nephritis specimens, but not in any of the control sections (P = 0.006). The tubular MT score, defined as the median......INTRODUCTION: Metallothionein (MT) isoforms I + II are polypeptides with potent antioxidative and anti-inflammatory properties. In healthy kidneys, MT-I+II have been described as intracellular proteins of proximal tubular cells. The aim of the present study was to investigate whether the renal MT......-I+II expression profile is altered during lupus nephritis. METHODS: Immunohistochemistry was performed on renal biopsies from 37 patients with lupus nephritis. Four specimens of healthy renal tissue served as controls. Clinicopathological correlation studies and renal survival analyses were performed by means...
Simultaneous removal of humic acid/fulvic acid and lead from landfill leachate using magnetic graphene oxide

Energy Technology Data Exchange (ETDEWEB)

Zhang, Jing [College of Environmental Science and Engineering, Key Laboratory of Environmental Biology and Pollution Control, Ministry of Education, Hunan University, Changsha 410082 (China); Gong, Ji-Lai, E-mail: jilaigong@gmail.com [College of Environmental Science and Engineering, Key Laboratory of Environmental Biology and Pollution Control, Ministry of Education, Hunan University, Changsha 410082 (China); Zenga, Guang-Ming, E-mail: zgming@hnu.edu.cn [College of Environmental Science and Engineering, Key Laboratory of Environmental Biology and Pollution Control, Ministry of Education, Hunan University, Changsha 410082 (China); Ou, Xiao-Ming [China National Engineering Research Center for Agrochemicals, Hunan Research Institute of Chemical Industry, Changsha 410014 (China); Jiang, Yan; Chang, Ying-Na; Guo, Min; Zhang, Chang; Liu, Hong-Yu [College of Environmental Science and Engineering, Key Laboratory of Environmental Biology and Pollution Control, Ministry of Education, Hunan University, Changsha 410082 (China)

2016-05-01

Highlights: • Magnetic graphene oxide was synthesized and used to simultaneously remove HA/FA and Pb(II) from landfill leachate. • In HA-Pb(II) system, Pb(II) removal rapidly increased to the maximum (about 87%) and considerably decreased with increasing HA concentration. However, in FA-Pb(II) system, Pb(II) removal slightly increased and remained constant as FA concentration increased. • In binary system, the removal efficiency of HA/FA by MGO was enhanced due to the increase of Pb(II) concentration. • In landfill leachate, MGO showed considerable removal efficiency for both Pb(II) and HA/FA. - Abstract: The elimination of organic matters and heavy metals in landfill leachate remains a longstanding challenge in wastewater treatment. In this study, magnetic graphene oxide (MGO) was synthesized and investigated to explore the possibility of applying in the simultaneous removal of HA/FA and Pb(II) from landfill leachate. MGO was characterized by Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) spectroscope. In single system, the sorption isotherm for FA on MGO at 25 °C were better described by Freundlich model than Langmuir and Temkin models with a maximum adsorption capacity of 72.38 mg/g. The isotherm data for HA at 25 °C was fitted well both Freundlich and Temkin models with a maximum adsorption capacity of 98.82 mg/g, while the isotherm data for Pb(II) at 25 °C was fitted well both Langmuir and Temkin models with a maximum adsorption capacity of 58.43 mg/g. In binary system, results showed that TOC removal (both in HA and FA) enhanced with increasing Pb(II). Furthermore, TOC removal enhancement caused by the increase of Pb(II) in HA-Pb(II) system was greater than that in FA-Pb(II), which was caused by HA possessing more substantial aromatic rings than FA. Noticeably, Pb(II) removal steeply increased to the maximum (about 87%) with increasing
Mistisisme dalam Perspektif Filsafat Analitik: Antara Wittgenstein dan Mehdi Ha'iri Yazdi

Directory of Open Access Journals (Sweden)

M. Hidayatullah S.

2007-12-01

Full Text Available Language is a principal instrument for philosopher and as a medium for analysis and reflection. Wittgenstein said, that the order analytical philosophy is to clean away the epistemology from the "good" terms but unclear in it interpretation. Every thing that can be thinks must be able spoken. The limits of my language mean the limits of my world. From this perspective mysticism and mystics experience is nin-sense, because nothing factual reference. From this case, the goal of this study is to find out the epistemology in mysticism and mystic experience. In the study we include the knowledge by presence (ilm al-hudhuri Ha 'iri as an approach. Ha 'iri said that there is a other knowledge which that can be explanations in limits of language and picture theory. This is knowledge by peresence, which by Wittgenstein aware as some thing which having difference language games from knowledge by correspondece.
Postoperative intra-abdominal collections using a sodium hyaluronate-carboxymethylcellulose (HA-CMC) barrier at the time of laparotomy for uterine or cervical cancers.

Science.gov (United States)

Leitao, Mario M; Byrum, Graham V; Abu-Rustum, Nadeem R; Brown, Carol L; Chi, Dennis S; Sonoda, Yukio; Levine, Douglas A; Gardner, Ginger J; Barakat, Richard R

2010-11-01

A prior analysis of patients undergoing laparotomy for ovarian malignancies at our institution revealed an increased rate of intra-abdominal collections using HA-CMC film during debulking surgery. The primary objective of the current study was to determine whether the use of HA-CMC is associated with the development of postoperative intra-abdominal collections in patients undergoing laparotomy for uterine or cervical malignancies. We retrospectively identified all laparotomies performed for these malignancies from 3/1/05 to 12/31/07. We identified cases involving the use of HA-CMC via billing records and operative reports. Intra-abdominal collections were defined as localized intraperitoneal fluid accumulations in the absence of re-accumulating ascites. We noted incidences of intra-abdominal collections, as well as other complications. Appropriate statistical tests were applied using SPSS 15.0. We identified 169 laparotomies in which HA-CMC was used and 347 in which HA-CMC was not used. The following were statistically similar in both cohorts: age, body mass index (BMI), primary site, surgery for recurrent disease, prior intraperitoneal surgery, and extent of current surgery. Intra-abdominal collections were seen in 6 (3.6%) of 169 HA-CMC cases compared to 10 (2.9%) of 347 non-HA-CMC cases (p=0.7). The rate of infected collections was similar in both groups (1.2% vs. 1.4%). In the subgroup that underwent tumor debulking, intra-abdominal collections were seen in 3 (11.5%) of 26 HA-CMC cases compared to 2 (5.4%) of 37 non-HA-CMC cases (p=0.6). HA-CMC use does not appear to be associated with postoperative intra-abdominal collections in patients undergoing laparotomy for uterine or cervical cancer. Copyright © 2010 Elsevier Inc. All rights reserved.
Modelling of Temperature Profiles and Transport Scaling in Auxiliary Heated Tokamaks

DEFF Research Database (Denmark)

Callen, J.D.; Christiansen, J.P.; Cordey, J.G.

1987-01-01

time , the heating effectiveness η, and the energy offset W(0). Considering both the temperature profile responses and the global transport scaling, the constant heat pinch or excess temperature gradient model is found to best characterize the present JET data. Finally, new methods are proposed......The temperature profiles produced by various heating profiles are calculated from local heat transport models. The models take the heat flux to be the sum of heat diffusion and a non-diffusive heat flow, consistent with local measurements of heat transport. Two models are developed analytically...... in detail: (i) a heat pinch or excess temperature gradient model with constant coefficients; and (ii) a non-linear heat diffusion coefficient (χ) model. Both models predict weak (lesssim20%) temperature profile responses to physically relevant changes in the heat deposition profile – primarily because...
Study on the changes of serum HA and CG levels in several diseases besides liver disorders

International Nuclear Information System (INIS)

Lu Zhiping; Ma Yunbao; Zhang Xiaoyi; Lv Weihua

2005-01-01

Objective: To study the changes of serum hyaluronic acid (HA) and cholyglycine (CG) in several diseases besides liver disorders. Methods: Serum HA and CG levels were measured with RIA in 78 patients with chronic liver diseases (with 70 controls), (84 patients with primary hepatic carcinoma ), 70 pediatric patients with various infections diseases (with 40 controls), 50 pediatric patients with recurrent respiratory infection (RRI, with 30 controls) and 428 pregnant women ( with 60 controls). In addition, RBC C 3 b receptor ratio (RBCC 3 bRR) and RBC immune-complex ratio (RBCICR) (both with yeast rosette method) as well as serum IgG,IgM,IgA,C 3 levels (with immunoturbidity test) were examined in the 50 RRI pediatric patients, ALT levels were examined in the 70 pediatric patients with infectious diseases and SF examined in the 78 patients with chronic liver diseases. Results: The serum CG, HA and SF levels in the three groups of patients with chronic liver diseases ( CPH, CAH, Cirrhosis) were significantly higher than those in the controls (all P 0.05). In patients with hepatic carcinoma, the CG and HA levels were positively correlated with the mortality at 4 months (P 0.05). For ALT, levels only increased in patients with hepatitis and typhoid fever with none in bacillary dysentery and mumps. In pediatric patients with RRI, RBCC 3 bRR (%), CG, C 3 , IgG levels were significantly higher than those in controls (P 0.05). Conclusion: Various infectious diseases in pediatric patients could induce mild liver damage, which was closely related to the depressed immune status. High CG and HA levels in liver cancer patients suggested high mortality at 4 months. Women in late pregnancy might harbour disturbances in CG metabolism and mild liver injury though without overt symptoms. (authors)
Conformational heterogeneity of the bacteriopheophytin electron acceptor HA in reaction centers from Rhodopseudomonas viridis revealed by Fourier transform infrared spectroscopy and site-directed mutagenesis.

Science.gov (United States)

Breton, J; Bibikova, M; Oesterhelt, D; Nabedryk, E

1999-08-31

The light-induced Fourier transform infrared (FTIR) difference spectra corresponding to the photoreduction of either the HA bacteriopheophytin electron acceptor (HA-/HA spectrum) or the QA primary quinone (QA-/QA spectrum) in photosynthetic reaction centers (RCs) of Rhodopseudomonas viridis are reported. These spectra have been compared for wild-type (WT) RCs and for two site-directed mutants in which the proposed interactions between the carbonyls on ring V of HA and the RC protein have been altered. In the mutant EQ(L104), the putative hydrogen bond between the protein and the 9-keto C=O of HA should be affected by changing Glu L104 to a Gln. In the mutant WF(M250), the van der Waals interactions between Trp M250 and the 10a-ester C=O of HA should be modified. The characteristic effects of both mutations on the FTIR spectra support the proposed interactions and allow the IR modes of the 9-keto and 10a-ester C=O of HA and HA- to be assigned. Comparison of the HA-/HA and QA-/QA spectra leads us to conclude that the QA-/QA IR signals in the spectral range above 1700 cm-1 are largely dominated by contributions from the electrostatic response of the 10a-ester C=O mode of HA upon QA photoreduction. A heterogeneity in the conformation of the 10a-ester C=O mode of HA in WT RCs, leading to three distinct populations of HA, appears to be related to differences in the hydrogen-bonding interactions between the carbonyls of ring V of HA and the RC protein. The possibility that this structural heterogeneity is related to the observed multiexponential kinetics of electron transfer and the implications for primary processes are discussed. The effect of 1H/2H exchange on the QA-/QA spectra of the WT and mutant RCs shows that neither Glu L104 nor any other exchangeable carboxylic residue changes appreciably its protonation state upon QA reduction.
Study on the clinical value of determination of serum adenosine deaminase (ADA) and HA, PIIIP levels for diagnosis of chronic hepatitis

International Nuclear Information System (INIS)

Ma Yun

2007-01-01

Objective: To explore the clinical value of determination of serum ADA and HA, PIIIP levels for diagnosis of chronic hepatitis. Methods: Serum ADA levels were determined with biochemistry and HA, PIIIP levels were detected with RIA in 36 patients with chronic hepatitis and 35 controls. Results: The levels of serum ADA, HA and PIIIP were significantly higher in the patients than those in controls (P<0.01). Serum ADA, HA, PIIIP levelers were mutually positively correlated (r=0.6117, 0.7134, P<0.01). Conclusion: Detection of serum ADA levels could reflect the liver reserve function and status of fibrosis. (authors)
Correlations between the in vitro and in vivo bioactivity of the Ti/HA composites fabricated by a powder metallurgy method.

Science.gov (United States)

Ning, Congqin; Zhou, Yu

2008-11-01

Ti/HA composites were successfully prepared by a powder metallurgy method and the effect of phase composition on the in vitro and in vivo bioactivity of the Ti/HA composites was investigated in the present study. The correlations between the in vitro and in vivo biological behaviors were highlighted. The results showed that the in vitro and in vivo bioactivity of the Ti/HA composites was dependent on their phase composition. The in vitro bioactivity of the Ti/HA composites was evaluated in simulated body fluid with ion concentrations similar to those of human plasma. After immersion in the simulated body fluid for a certain time, apatite precipitations formed on the surface of the composites with an initial titanium content of 50 and 70 wt.%, and no apatite was found on the surface of the composite with 30% titanium. Ti(2)O was responsible for the apatite formation on the surfaces of the composites. For in vivo analysis, Ti/HA cylinders were implanted in the metaphases of the rabbit femur. At the early stage of implantation, the new bone formed on the surface of the composite with 30% titanium was much less than that on the surfaces of the composites with 50% and 70% titanium. All the Ti/HA composites formed a chemical bone-bonding interface with the host bone by 6 months after implantation. The Ti/HA composites formed the bone-bonding interface with the surrounding bone through an apatite layer. The results in the present study suggested that the in vivo results agreed well with the in vitro results.
Response of Human Osteoblast to n-HA/PEEK—Quantitative Proteomic Study of Bio-effects of Nano-Hydroxyapatite Composite

Science.gov (United States)

Zhao, Minzhi; Li, Haiyun; Liu, Xiaochen; Wei, Jie; Ji, Jianguo; Yang, Shu; Hu, Zhiyuan; Wei, Shicheng

2016-03-01

Nano-sized hydroxyapatite (n-HA) is considered as a bio-active material, which is often mixed into bone implant material, polyetheretherketone (PEEK). To reveal the global protein expression modulations of osteoblast in response to direct contact with the PEEK composite containing high level (40%) nano-sized hydroxyapatite (n-HA/PEEK) and explain its comprehensive bio-effects, quantitative proteomic analysis was conducted on human osteoblast-like cells MG-63 cultured on n-HA/PEEK in comparison with pure PEEK. Results from quantitative proteomic analysis showed that the most enriched categories in the up-regulated proteins were related to calcium ion processes and associated functions while the most enriched categories in the down-regulated proteins were related to RNA process. This enhanced our understanding to the molecular mechanism of the promotion of the cell adhesion and differentiation with the inhibition of the cell proliferation on n-HA/PEEK composite. It also exhibited that although the calcium ion level of incubate environment hadn’t increased, merely the calcium fixed on the surface of material had influence to intracellular calcium related processes, which was also reflect by the higher intracellular Ca2+ concentration of n-HA/PEEK. This study could lead to more comprehensive cognition to the versatile biocompatibility of composite materials. It further proves that proteomics is useful in new bio-effect discovery.
Response of Human Osteoblast to n-HA/PEEK—Quantitative Proteomic Study of Bio-effects of Nano-Hydroxyapatite Composite

Science.gov (United States)

Zhao, Minzhi; Li, Haiyun; Liu, Xiaochen; Wei, Jie; Ji, Jianguo; Yang, Shu; Hu, Zhiyuan; Wei, Shicheng

2016-01-01

Nano-sized hydroxyapatite (n-HA) is considered as a bio-active material, which is often mixed into bone implant material, polyetheretherketone (PEEK). To reveal the global protein expression modulations of osteoblast in response to direct contact with the PEEK composite containing high level (40%) nano-sized hydroxyapatite (n-HA/PEEK) and explain its comprehensive bio-effects, quantitative proteomic analysis was conducted on human osteoblast-like cells MG-63 cultured on n-HA/PEEK in comparison with pure PEEK. Results from quantitative proteomic analysis showed that the most enriched categories in the up-regulated proteins were related to calcium ion processes and associated functions while the most enriched categories in the down-regulated proteins were related to RNA process. This enhanced our understanding to the molecular mechanism of the promotion of the cell adhesion and differentiation with the inhibition of the cell proliferation on n-HA/PEEK composite. It also exhibited that although the calcium ion level of incubate environment hadn’t increased, merely the calcium fixed on the surface of material had influence to intracellular calcium related processes, which was also reflect by the higher intracellular Ca2+ concentration of n-HA/PEEK. This study could lead to more comprehensive cognition to the versatile biocompatibility of composite materials. It further proves that proteomics is useful in new bio-effect discovery. PMID:26956660
The sunflower transcription factor HaHB11 improves yield, biomass and tolerance to flooding in transgenic Arabidopsis plants.

Science.gov (United States)

Cabello, Julieta V; Giacomelli, Jorge I; Piattoni, Claudia V; Iglesias, Alberto A; Chan, Raquel L

2016-03-20

HaHB11 is a member of the sunflower homeodomain-leucine zipper I subfamily of transcription factors. The analysis of a sunflower microarray hybridized with RNA from HaHB11-transformed leaf-disks indicated the regulation of many genes encoding enzymes from glycolisis and fermentative pathways. A 1300bp promoter sequence, fused to the GUS reporter gene, was used to transform Arabidopsis plants showing an induction of expression after flooding treatments, concurrently with HaHB11 regulation by submergence in sunflower. Arabidopsis transgenic plants expressing HaHB11 under the control of the CaMV 35S promoter and its own promoter were obtained and these plants exhibited significant increases in rosette and stem biomass. All the lines produced more seeds than controls and particularly, those of high expression level doubled seeds yield. Transgenic plants also showed tolerance to flooding stress, both to submergence and waterlogging. Carbohydrates contents were higher in the transgenics compared to wild type and decreased less after submergence treatments. Finally, transcript levels of selected genes involved in glycolisis and fermentative pathways as well as the corresponding enzymatic activities were assessed both, in sunflower and transgenic Arabidopsis plants, before and after submergence. Altogether, the present work leads us to propose HaHB11 as a biotechnological tool to improve crops yield, biomass and flooding tolerance. Copyright © 2016 Elsevier B.V. All rights reserved.
Cognitive Dysfunction Is Worse among Pediatric Patients with Bipolar Disorder Type I than Type II

Science.gov (United States)

Schenkel, Lindsay S.; West, Amy E.; Jacobs, Rachel; Sweeney, John A.; Pavuluri, Mani N.

2012-01-01

Background: Impaired profiles of neurocognitive function have been consistently demonstrated among pediatric patients with bipolar disorder (BD), and may aid in the identification of endophenotypes across subtypes of the disorder. This study aims to determine phenotypic cognitive profiles of patients with BD Type I and II. Methods: Subjects (N =…
The effect of partially stabilized zirconia on the biological properties of HA/HDPE composites in vitro.

Science.gov (United States)

Sadi, A Yari; Shokrgozar, M A; Homaeigohar, S Sh; Hosseinalipour, M; Khavandi, A; Javadpour, J

2006-05-01

The effect of partially stabilized zirconia (PSZ) on the biological properties of the hyroxyapatite - high density polyethylene (HA/HDPE) composites was studied by investigating the simultaneous effect of hydroxyapatite and PSZ volume fractions on the in vitro response of human osteoblast cells. The biocompatibility of composite samples with different volume fraction of HA and PSZ powders was assessed by proliferation, alkaline phosphatase (ALP) and cell attachment assays on the osteoblast cell line (G-292) in different time periods. The effect of composites on the behavior of G-292 cells was compared with those of HDPE and TPS (Tissue Culture Poly Styrene as negative control) samples. Results showed a higher proliferation rate of G-292 cells in the presence of composite samples as compared to the HDPE sample after 7 and 14 days of incubation period. ALP production rate in all composite samples was higher than HDPE and TPS samples. The number of adhered cells on the composite samples was higher than the number adhered on the HDPE and TPS samples after the above mentioned incubation periods. These findings indicates that the addition of PSZ does not have any adverse affect on the biocompatibility of HA/HDPE composites. In fact in some experiments PSZ added HA/HDPE composites performed better in proliferation, differentiation and attachment of osteoblastic cells.

Trichomonas vaginalis Induces SiHa Cell Apoptosis by NF-κB Inactivation via Reactive Oxygen Species

Science.gov (United States)

Quan, Juan-Hua; Kang, Byung-Hun; Yang, Jung-Bo; Rhee, Yun-Ee; Noh, Heung-Tae; Choi, In-Wook; Cha, Guang-Ho; Yuk, Jae-Min

2017-01-01

Trichomonas vaginalis induces apoptosis in host cells through various mechanisms; however, little is known about the relationship between apoptosis, reactive oxygen species (ROS), and NF-κB signaling pathways in the cervical mucosal epithelium. Here, we evaluated apoptotic events, ROS production, and NF-κB activity in T. vaginalis-treated cervical mucosal epithelial SiHa cells, with or without specific inhibitors, using fluorescence microscopy, DNA fragmentation assays, subcellular fractionation, western blotting, and luciferase reporter assay. SiHa cells treated with live T. vaginalis at a multiplicity of infection of 5 (MOI 5) for 4 h produced intracellular and mitochondrial ROS in a parasite-load-dependent manner. Incubation with T. vaginalis caused DNA fragmentation, cleavage of caspase 3 and PARP, and release of cytochrome c into the cytoplasm. T. vaginalis-treated SiHa cells showed transient early NF-κB p65 nuclear translocation, which dramatically dropped at 4 h after treatment. Suppression of NF-κB activity was dependent on parasite burden. However, treatment with the ROS scavenger, N-acetyl-C-cysteine (NAC), reversed the effect of T. vaginalis on apoptosis and NF-κB inactivation in SiHa cells. Taken together, T. vaginalis induces apoptosis in human cervical mucosal epithelial cells by parasite-dose-dependent ROS production through an NF-κB-regulated, mitochondria-mediated pathway. PMID:29410962
Trichomonas vaginalis Induces SiHa Cell Apoptosis by NF-κB Inactivation via Reactive Oxygen Species

Directory of Open Access Journals (Sweden)

Juan-Hua Quan

2017-01-01

Full Text Available Trichomonas vaginalis induces apoptosis in host cells through various mechanisms; however, little is known about the relationship between apoptosis, reactive oxygen species (ROS, and NF-κB signaling pathways in the cervical mucosal epithelium. Here, we evaluated apoptotic events, ROS production, and NF-κB activity in T. vaginalis-treated cervical mucosal epithelial SiHa cells, with or without specific inhibitors, using fluorescence microscopy, DNA fragmentation assays, subcellular fractionation, western blotting, and luciferase reporter assay. SiHa cells treated with live T. vaginalis at a multiplicity of infection of 5 (MOI 5 for 4 h produced intracellular and mitochondrial ROS in a parasite-load-dependent manner. Incubation with T. vaginalis caused DNA fragmentation, cleavage of caspase 3 and PARP, and release of cytochrome c into the cytoplasm. T. vaginalis-treated SiHa cells showed transient early NF-κB p65 nuclear translocation, which dramatically dropped at 4 h after treatment. Suppression of NF-κB activity was dependent on parasite burden. However, treatment with the ROS scavenger, N-acetyl-C-cysteine (NAC, reversed the effect of T. vaginalis on apoptosis and NF-κB inactivation in SiHa cells. Taken together, T. vaginalis induces apoptosis in human cervical mucosal epithelial cells by parasite-dose-dependent ROS production through an NF-κB-regulated, mitochondria-mediated pathway.
Fabrication and bioactivity behavior of HA/bioactive glass composites in the presence of calcium hexaboride

Energy Technology Data Exchange (ETDEWEB)

El-Bassyouni, Gehan T.; Beherei, Hanan H. [Biomaterials Dept., National Research Centre (NRC), Dokki, Cairo (Egypt); Mohamed, Khaled R., E-mail: kh_rezk1966@yahoo.com [Biomaterials Dept., National Research Centre (NRC), Dokki, Cairo (Egypt); Kenawy, Sayed H. [Ceramics Dept., National Research Centre (NRC), Dokki, Cairo (Egypt)

2016-06-01

In the current study, composites were prepared using both the synthesized nano-sized hydroxyapatite (HA), bioactive glass (BG) powders (obtained by the traditional melt-quenching route) together with the purchased nano-sized calcium hexaboride (CB) with different ratios and were fired at 1250 °C. The structure and composition of the solid reaction products were analyzed using X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy; scanning electron microscope (SEM) coupled with energy dispersive spectroscopy (EDS), transmission electron microscope (TEM) techniques and compressive strength. The mechanical testing was to designate the role of the CB in improving the mechanical property of the prepared composites. In vitro bioactivity of the prepared composites was assessed by soaking in the simulated body fluid (SBF) at 37 ± 0.5 °°C for 10 days. The effect of different ratios of the three components (CB, HA & BG) on the bioactivity properties was assessed to explore the possibility of enhancing such property to perform in vitro imitations of in vivo conditions in the future. It can be pointed out that the Si-HA content in the composition showed outstanding in vitro bioactivity than pure hydroxyapatite which could be attributed to the excellent bioactivity of the synthesized composites. - Highlights: • The prepared of nano-composites containing CB, HA and BG powders were achieved. • The addition of CB powder enhanced the compressive strength for all the composites. • The composites containing high BG and CB contents improved formation of bone-like apatite layer.
Fabrication and bioactivity behavior of HA/bioactive glass composites in the presence of calcium hexaboride

International Nuclear Information System (INIS)

El-Bassyouni, Gehan T.; Beherei, Hanan H.; Mohamed, Khaled R.; Kenawy, Sayed H.

2016-01-01

In the current study, composites were prepared using both the synthesized nano-sized hydroxyapatite (HA), bioactive glass (BG) powders (obtained by the traditional melt-quenching route) together with the purchased nano-sized calcium hexaboride (CB) with different ratios and were fired at 1250 °C. The structure and composition of the solid reaction products were analyzed using X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy; scanning electron microscope (SEM) coupled with energy dispersive spectroscopy (EDS), transmission electron microscope (TEM) techniques and compressive strength. The mechanical testing was to designate the role of the CB in improving the mechanical property of the prepared composites. In vitro bioactivity of the prepared composites was assessed by soaking in the simulated body fluid (SBF) at 37 ± 0.5 °°C for 10 days. The effect of different ratios of the three components (CB, HA & BG) on the bioactivity properties was assessed to explore the possibility of enhancing such property to perform in vitro imitations of in vivo conditions in the future. It can be pointed out that the Si-HA content in the composition showed outstanding in vitro bioactivity than pure hydroxyapatite which could be attributed to the excellent bioactivity of the synthesized composites. - Highlights: • The prepared of nano-composites containing CB, HA and BG powders were achieved. • The addition of CB powder enhanced the compressive strength for all the composites. • The composites containing high BG and CB contents improved formation of bone-like apatite layer.
Type II critical phenomena of neutron star collapse

International Nuclear Information System (INIS)

Noble, Scott C.; Choptuik, Matthew W.

2008-01-01

We investigate spherically symmetric, general relativistic systems of collapsing perfect fluid distributions. We consider neutron star models that are driven to collapse by the addition of an initially 'ingoing' velocity profile to the nominally static star solution. The neutron star models we use are Tolman-Oppenheimer-Volkoff solutions with an initially isentropic, gamma law equation of state. The initial values of (1) the amplitude of the velocity profile, and (2) the central density of the star, span a parameter space, and we focus only on that region that gives rise to type II critical behavior, wherein black holes of arbitrarily small mass can be formed. In contrast to previously published work, we find that--for a specific value of the adiabatic index (Γ=2)--the observed type II critical solution has approximately the same scaling exponent as that calculated for an ultrarelativistic fluid of the same index. Further, we find that the critical solution computed using the ideal-gas equations of state asymptotes to the ultrarelativistic critical solution.
CRED Shallow CTD Profiles; Lisianski Island, Northwestern Hawaiian Islands (Papahanaumokuakea Marine National Monument); Cruise: HA1007, Data Date Range: 20100923-20100924 (NODC Accession 0039382).

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
CRED Shallow CTD Profiles; Kure Atoll, Northwestern Hawaiian Islands (Papahanaumokuakea Marine National Monument); Cruise: HA1007, Data Date Range: 20100919-20100920 (NODC Accession 0039382).

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
CRED Shallow CTD Profiles; Asuncion Island, Commonwealth of the Northern Mariana Islands; Cruise: HA1101_LEGII, Data Date Range: 20110414-20110414. (NODC Accession 0107470)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...
Haïti-Quisqueya: une double insularité

Directory of Open Access Journals (Sweden)

Jean-Marie THÉODAT

1998-09-01

Full Text Available Deux pays se partagent une île coupée par une frontière méridienne de plus de 300 km: Haïti à l'ouest, Quisqueya à l'est. Cette coupure est l'héritage du partage colonial de l'île entre la France et l'Espagne, et le résultat de la dynamique spatiale contrastée des deux nations qui y sont nées et qui ont eu recours à des principes d'encadrement distincts.De là un sentiment de double insularité, chaque pays se comportant vis-à-vis de son voisin comme s'ils étaient situés sur deux îles séparées.
The hormesis effect of plasma-elevated intracellular ROS on HaCaT cells

Science.gov (United States)

Szili, Endre J.; Harding, Frances J.; Hong, Sung-Ha; Herrmann, Franziska; Voelcker, Nicolas H.; Short, Robert D.

2015-12-01

We have examined the link between ionized-gas plasma delivery of reactive oxygen species (ROS) to immortalized keratinocyte (HaCaT) cells and cell fate, defined in terms of cell viability versus death. Phospholipid vesicles were used as cell mimics to measure the possible intracellular ROS concentration, [ROSi], delivered by various plasma treatments. Cells were exposed to a helium cold atmospheric plasma (CAP) jet for different plasma exposure times (5-60 s) and gas flow rates (50-1000 ml min-1). Based upon the [ROSi] data we argue that plasma-generated ROS in the cell culture medium can readily diffuse into real cells. Plasma exposure that equated to an [ROSi] in the range of 3.81 × 10-10-9.47 × 10-8 M, measured at 1 h after the plasma exposure, resulted in increased cell viability at 72 h; whereas a higher [ROSi] at 1 h decreased cell viability after 72 h of culture. This may be because of the manner in which the ROS are delivered by the plasma: HaCaT cells better tolerate a low ROS flux over an extended plasma exposure period of 1 min, compared to a high flux delivered in a few seconds, although the final [ROSi] may be the same. Our results suggest that plasma stimulation of HaCaT cells follows the principle of hormesis.
Bone Regeneration Using a Mixture of Silicon-Substituted Coral HA and β-TCP in a Rat Calvarial Bone Defect Model

Directory of Open Access Journals (Sweden)

Jiyeon Roh

2016-02-01

Full Text Available The demand of bone graft materials has been increasing. Among various origins of bone graft materials, natural coral composed of up to 99% calcium carbonate was chosen and converted into hydroxyapatite (HA; silicon was then substituted into the HA. Then, the Si-HA was mixed with β-tricalcium phosphate (TCP in the ratios 100:0 (S100T0, 70:30 (S70T30, 60:40 (S60T40, and 50:50 (S50T50. The materials were implanted for four and eight weeks in a rat calvarial bone defect model (8 mm. The MBCPTM (HA:β-TCP = 60:40, Biomatalante, Vigneux de Bretagne, France was used as a control. After euthanasia, the bone tissue was analyzed by making histological slides. From the results, S60T40 showed the fastest bone regeneration in four weeks (p < 0.05. In addition, S60T40, S50T50, and MBCPTM showed significant new bone formation in eight weeks (p < 0.05. In conclusion, Si-HA/TCP showed potential as a bone graft material.
Clinical significance of combined determination of the changes of the serum ADA, HA and PIIIP levels in patients with hepatitis B

International Nuclear Information System (INIS)

Wang Xiaoyan

2009-01-01

Objective: To explore the clinical significance of the changes of serum ADA, HA and PIIIP levels in patients with hepatitis B. Methods: Serum adenosine deaminase (ADA) (with biochemistry) and HA PIIIP (with RIA) levels were determined in 102 patients with hepatitis B of various stages and 35 controls. Results: Serum ADA, HA and PIIIP levels in patients with hepatitis B were significantly higher than those in controls (P<0.01). Particularly in patients with cirrhosis (n=13) (P<0.001). Conclusion: The serum ADA, HA and PIIIP levels were closely related to the diseases process of hepatitis B and were of great clinical values. (authors)
WISC-IV and WIAT-II Profiles in Children with High-Functioning Autism

Science.gov (United States)

Mayes, Susan Dickerson; Calhoun, Susan L.

2008-01-01

Children with high-functioning autism earned above normal scores on the Wechsler Intelligence Scale for Children-Fourth Edition (WISC-IV) Perceptual Reasoning and Verbal Comprehension Indexes and below normal scores on the Working Memory and Processing Speed Indexes and Wechsler Individual Achievement Test-Second Edition (WIAT-II) Written…
Dissolved inorganic carbon, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the Ryofu Maru II in the Bismarck Sea, North Pacific Ocean and others from 1994-07-07 to 1994-08-25 (NODC Accession 0115017)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115017 includes chemical, discrete sample, physical and profile data collected from Ryofu Maru II in the Bismarck Sea, North Pacific Ocean,...
Caelyx (TM) in malignant mesothelioma : A phase II EORTC study

NARCIS (Netherlands)

Baas, P; van Meerbeeck, J; Groen, H; Schouwink, H; Burgers, S; Daamen, S; Giaccone, G

Background: The use of doxorubicin has shown some activity in malignant mesothelioma but prolonged administration is hampered by cardiotoxicity. Caelyx(TM), a new liposomal and pegylated form of doxorubicin has shown a better pharmacokinetic and toxic profile then doxorubicin. In a phase II study,
Characterization of hydroxybenzoic acid chelating resins: equilibrium, kinetics, and isotherm profiles for Cd(II and Pb(II uptake

Directory of Open Access Journals (Sweden)

BHAVNA A. SHAH

2011-06-01

Full Text Available Chelating ion-exchange resins were synthesized by polycondensation of ortho/para hydroxybenzoic acid with resorcinol/catechol employing formaldehyde as cross-linking agent at 80±5 °C in DMF. The resins were characterized by FTIR and XRD. The uptake behaviour of synthesized resins for Cd(II and Pb(II ions have been studied depending on contact time, pH, metal ion concentration and temperature. The sorption data obtained at optimized conditions were analyzed by the Langmuir and Freundlich isotherms. Experimental data of all metal–resin system were best represented by the Freundlich isotherm. The maximum obtained sorption capacity for cadmium was 69.53 mg g-1 and 169.32 mg g-1 for Lead. The adsorption process follows first order kinetics and the specific rate constant Kr was obtained by the application of the Lagergan equation. Thermodynamic parameters ∆Gads, ∆Sads and ∆Hads were calculated for the metal–resin systems. The external diffusion rate constant (KS and the intra-particle diffusion rate constant (Kid were calculated by the Spahn–Schlunder and Weber–Morris models, respectively. The sorption process was found to follow an intra-particle diffusion phenomenon.
Structural trends in a series of isostructural lanthanide-copper metallacrown sulfates (Ln(III) = Pr, Nd, Sm, Eu, Gd, Dy and Ho): hexaaquapentakis[μ3-glycinehydroxamato(2-)]sulfatopentacopper(II)lanthanide(III) heptaaquapentakis[μ3-glycinehydroxamato(2-)]sulfatopentacopper(II)lanthanide(III) sulfate hexahydrate.

Science.gov (United States)

Pavlishchuk, Anna V; Kolotilov, Sergey V; Fritsky, Igor O; Zeller, Matthias; Addison, Anthony W; Hunter, Allen D

2011-07-01

The seven isostructural complexes, [Cu(5)Ln(C(2)H(4)N(2)O(2))(5)(SO(4))(H(2)O)(6.5)](2)(SO(4))·6H(2)O, where Ln(III) = Pr, Nd, Sm, Eu, Gd, Dy and Ho, are representatives of the 15-metallacrown-5 family. Each dianion of glycinehydroxamic acid (GlyHA) links two Cu(II) cations forming a cyclic [CuGlyHA](5) frame. The Ln(III) cations are located at the centre of the [CuGlyHA](5) rings and are bound by the five hydroxamate O atoms in the equatorial plane. Five water molecules are coordinated to Cu(II) cations, and one further water molecule, located close to an inversion centre between two adjacent [Cu(5)Ln(GlyHA)(5)](2+) cations, is disordered around this inversion centre and coordinated to a Cu(II) cation of either the first or second metallacrown ether. Another water molecule and one of the two crystallographically independent sulfate anions are coordinated, the latter in a bidentate fashion, to the Ln(III) cation in the axial positions. The second sulfate anion is not coordinated to the cation, but is located in an interstitial position on a crystallographic inversion centre, thus leading to disorder of the O atoms around the centre of inversion. The Ln-O bond distances follow the trend of the lanthanide contraction. The apical Ln-O bond distances are very close to the sums of the ionic radii. However, the Ln-O distances within the metallacrown units are slightly compressed and the Ln(III) cations protrude significantly from the plane of the otherwise flat metallacrown ligand, thus indicating that the cavity is somewhat too small to accommodate the Ln(III) ions comfortably. This effect decreases with the size of the lanthanide cation from complex (I) (Ln(III) = Pr; 0.459) to complex (VII) (Ln(III) = Ho; 0.422), which indicates that the smaller lanthanide cations fit the cavity of the pentacopper metallacrown ring better than the larger ones. The diminished contraction of Ln-O distances within the metallacrown planes leads to an aniostropic contraction of the unit
Metallothionein-I plus II and receptor megalin are altered in relation to oxidative stress in cerebral lymphomas

DEFF Research Database (Denmark)

Pedersen, M.O.; Hansen, P.B.; Nielsen, Signe Ledou

2010-01-01

. This article characterizes the histopathology and expression profiles of metallothionein-I + II (MT-I + II) and their receptor megalin along with proliferation, oxidative stress, and apoptosis in PCNSL and in central nervous system (CNS) lymphomas due to relapse from DLBCL (collectively referred to as CNS...
The Effect of Titanium Dioxide (TiO2) Nanoparticles on Hydroxyapatite (HA)/TiO2 Composite Coating Fabricated by Electrophoretic Deposition (EPD)

Science.gov (United States)

Amirnejad, M.; Afshar, A.; Salehi, S.

2018-05-01

Composite coatings of Hydroxyapatite (HA) with ceramics, polymers and metals are used to modify the surface structure of implants. In this research, HA/TiO2 composite coating was fabricated by electrophoretic deposition (EPD) on 316 stainless steel substrate. HA/TiO2 composite coatings with 5, 10 and 20 wt.% of TiO2, deposited at 40 V and 90 s as an optimum condition. The samples coated at this condition led to an adherent, continuous and crack-free coating. The influence of TiO2 content was studied by performing different characterization methods such as scanning electron microscopy (SEM), energy dispersive x-ray spectroscopy (EDS), corrosion resistance in simulated body fluid (SBF), coating's dissolution rate in physiological solution and bond strength to the substrate. The results showed that the higher amount of TiO2 in the composite coating led to increase in bond strength of coating to stainless steel substrate from 3 MPa for HA coating to 5.5 MPa for HA-20 wt.% TiO2 composite coating. In addition, it caused to reduction of corrosion current density of samples in the SBF solution from 18.92 μA/cm2 for HA coating to 6.35 μA/cm2 for HA-20 wt.% TiO2 composite coating.
The Effect of Titanium Dioxide (TiO2) Nanoparticles on Hydroxyapatite (HA)/TiO2 Composite Coating Fabricated by Electrophoretic Deposition (EPD)

Science.gov (United States)

Amirnejad, M.; Afshar, A.; Salehi, S.

2018-04-01

Composite coatings of Hydroxyapatite (HA) with ceramics, polymers and metals are used to modify the surface structure of implants. In this research, HA/TiO2 composite coating was fabricated by electrophoretic deposition (EPD) on 316 stainless steel substrate. HA/TiO2 composite coatings with 5, 10 and 20 wt.% of TiO2, deposited at 40 V and 90 s as an optimum condition. The samples coated at this condition led to an adherent, continuous and crack-free coating. The influence of TiO2 content was studied by performing different characterization methods such as scanning electron microscopy (SEM), energy dispersive x-ray spectroscopy (EDS), corrosion resistance in simulated body fluid (SBF), coating's dissolution rate in physiological solution and bond strength to the substrate. The results showed that the higher amount of TiO2 in the composite coating led to increase in bond strength of coating to stainless steel substrate from 3 MPa for HA coating to 5.5 MPa for HA-20 wt.% TiO2 composite coating. In addition, it caused to reduction of corrosion current density of samples in the SBF solution from 18.92 μA/cm2 for HA coating to 6.35 μA/cm2 for HA-20 wt.% TiO2 composite coating.

A Human Clinical, Histological, Histomorphometrical, and Radiographical Study on Biphasic HA-Beta-TCP 30/70 in Maxillary Sinus Augmentation.

Science.gov (United States)

Mangano, Carlo; Sinjari, Bruna; Shibli, Jamil A; Mangano, Francesco; Hamisch, Sabine; Piattelli, Adriano; Perrotti, Vittoria; Iezzi, Giovanna

2015-06-01

By mixing hydroxyapatite (HA) and tricalcium phosphate (TCP), biphasic calcium phosphate ceramics can be obtained, and by varying their ratio it is possible to tailor the characteristics of the biomaterial. The aim of the present human study was to evaluate the histological and radiographical aspects of bone formation in maxillary sinus augmentation using a 30/70 HA-beta-TCP with a reticular structure. A total of 12 patients, undergoing two-stage sinus augmentation procedure using HA-beta-TCP at a ratio of 30/70, were included in the present study. After a 6-month healing period, during implant insertion, radiographical analysis was performed, and then the bone core biopsies were harvested and processed for histology. At radiographic evaluation, the bone gain was on average 6.85 ± 0.60 mm. HA-beta-TCP 30/70 appeared to be lined by newly formed bone, with no gaps at the interface. The histomorphometric analysis revealed 26 ± 2% of residual grafted biomaterial, 29 ± 3% of newly formed bone, and 45 ± 2% of marrow spaces. The present results indicate histologically the high biocompatibility and osteoconductivity of HA-beta-TCP 30/70, and clinically its successful use for sinus augmentation procedures. © 2013 Wiley Periodicals, Inc.
Narrow power deposition profiles on the JET divertor target

International Nuclear Information System (INIS)

Lingertat, J.; Laux, M.; Monk, R.

2001-01-01

One of the key unresolved issues in the design of a future fusion reactor is the power handling capability of the divertor target plates. Earlier we reported on the existence of narrow power deposition profiles in JET, obtained mainly from Langmuir probe measurements. We repeated these measurements in the MkI, MkII and MkIIGB divertor configurations with an upgraded probe system, which allowed us to study the profile shape in more detail. The main results of this study are: In NB heated discharges the electron temperature and power flux at the outer target show a distinct peak of ∼5 mm half-width near the separatrix strike point. The corresponding profiles on the inner target do not show a similar feature. The height of the narrow peak increases with NB heating power and decreases with deuterium and impurity gas puffing. Ion orbit losses are suggested as a possible explanation of the observed profile shape
Synthesis and characterisation of Cu(II), Ni(II), Mn(II), Zn(II) and VO(II ...

Indian Academy of Sciences (India)

Unknown

Synthesis and characterisation of Cu(II), Ni(II), Mn(II), Zn(II) and VO(II) Schiff base complexes derived from o-phenylenediamine and acetoacetanilide. N RAMAN*, Y PITCHAIKANI RAJA and A KULANDAISAMY. Department of Chemistry, VHNSN College, Virudhunagar 626 001, India e-mail: ra_man@123india.com.
Type II collagen-hyaluronan hydrogel – a step towards a scaffold for intervertebral disc tissue engineering

Directory of Open Access Journals (Sweden)

L Calderon

2010-09-01

Full Text Available Intervertebral disc regeneration strategies based on stem cell differentiation in combination with the design of functional scaffolds is an attractive approach towards repairing/regenerating the nucleus pulposus. The specific aim of this study was to optimise a composite hydrogel composed of type II collagen and hyaluronic acid (HA as a carrier for mesenchymal stem cells. Hydrogel stabilisation was achieved by means of 1-ethyl-3(3-dimethyl aminopropyl carbodiimide (EDC and N-hydroxysuccinimide (NHS cross-linking. Optimal hydrogel properties were determined by investigating different concentrations of EDC (8mM, 24mM and 48mM. Stable hydrogels were obtained independent of the concentration of carbodiimide used. The hydrogels cross-linked by the lowest concentration of EDC (8mM demonstrated high swelling properties. Additionally, improved proliferation of seeded rat mesenchymal stem cells (rMSCs and hydrogel stability levels in culture were observed with this 8mM cross-linked hydrogel. Results from this study indicate that EDC/NHS (8mM cross-linked type II collagen/HA hydrogel was capable of supporting viability of rMSCs, and furthermore their differentiation into a chondrogenic lineage. Further investigations should be conducted to determine its potential as scaffold for nucleus pulposus regeneration/repair.
In vitro study of RRS HA injectable mesotherapy/biorevitalization product on human skin fibroblasts and its clinical utilization.

Science.gov (United States)

Deglesne, Pierre-Antoine; Arroyo, Rodrigo; Ranneva, Evgeniya; Deprez, Philippe

2016-01-01

Mesotherapy/biorevitalization with hyaluronic acid (HA) is a treatment approach currently used for skin rejuvenation. Various products with a wide range of polycomponent formulations are available on the market. Most of these formulations contain noncross-linked HA in combination with a biorevitalization cocktail, formed by various amounts of vitamins, minerals, amino acids, nucleotides, coenzymes, and antioxidants. Although ingredients are very similar among the different products, in vitro and clinical effects may vary substantially. There is a real need for better characterization of these products in terms of their action on human skin or in vitro skin models. In this study, we analyzed the effect of the RRS(®) (Repairs, Refills, Stimulates) HA injectable medical device on human skin fibroblasts in vitro. Skin fibroblast viability and its capacity to induce the production of key extracellular matrix were evaluated in the presence of different concentrations of RRS HA injectable. Viability was evaluated through colorimetric MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay, and key extracellular matrix genes, type I collagen and elastin, were quantified by quantitative polymerase chain reaction. Results demonstrated that RRS HA injectable could promote human skin fibroblast viability (+15%) and increase fibroblast gene expression of type I collagen and elastin by 9.7-fold and 14-fold in vitro, respectively. These results demonstrate that mesotherapy/biorevitalization products can, at least in vitro, effectively modulate human skin fibroblasts.
Biologically active new Fe(II, Co(II, Ni(II, Cu(II, Zn(II and Cd(II complexes of N-(2-thienylmethylenemethanamine

Directory of Open Access Journals (Sweden)

C. SPÎNU

2008-04-01

Full Text Available Iron(II, cobalt(II, nickel (II, copper (II, zinc(II and cadmium(II complexes of the type ML2Cl2, where M is a metal and L is the Schiff base N-(2-thienylmethylenemethanamine (TNAM formed by the condensation of 2-thiophenecarboxaldehyde and methylamine, were prepared and characterized by elemental analysis as well as magnetic and spectroscopic measurements. The elemental analyses suggest the stoichiometry to be 1:2 (metal:ligand. Magnetic susceptibility data coupled with electronic, ESR and Mössbauer spectra suggest a distorted octahedral structure for the Fe(II, Co(II and Ni(II complexes, a square-planar geometry for the Cu(II compound and a tetrahedral geometry for the Zn(II and Cd(II complexes. The infrared and NMR spectra of the complexes agree with co-ordination to the central metal atom through nitrogen and sulphur atoms. Conductance measurements suggest the non-electrolytic nature of the complexes, except for the Cu(II, Zn(II and Cd(II complexes, which are 1:2 electrolytes. The Schiff base and its metal chelates were screened for their biological activity against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa and the metal chelates were found to possess better antibacterial activity than that of the uncomplexed Schiff base.
Technology transfer and scale-up of the Flublok recombinant hemagglutinin (HA) influenza vaccine manufacturing process.

Science.gov (United States)

Buckland, Barry; Boulanger, Robert; Fino, Mireli; Srivastava, Indresh; Holtz, Kathy; Khramtsov, Nikolai; McPherson, Clifton; Meghrous, Jamal; Kubera, Paul; Cox, Manon M J

2014-09-22

Multiple different hemagglutinin (HA) protein antigens have been reproducibly manufactured at the 650L scale by Protein Sciences Corporation (PSC) based on an insect cell culture with baculovirus infection. Significantly, these HA protein antigens were produced by the same Universal Manufacturing process as described in the biological license application (BLA) for the first recombinant influenza vaccine approved by the FDA (Flublok). The technology is uniquely designed so that a change in vaccine composition can be readily accommodated from one HA protein antigen to another one. Here we present a vaccine candidate to combat the recently emerged H7N9 virus as an example starting with the genetic sequence for the required HA, creation of the baculovirus and ending with purified protein antigen (or vaccine component) at the 10L scale accomplished within 38 days under GMP conditions. The same process performance is being achieved at the 2L, 10L, 100L, 650L and 2500L scale. An illustration is given of how the technology was transferred from the benchmark 650L scale facility to a retrofitted microbial facility at the 2500L scale within 100 days which includes the time for facility engineering changes. The successful development, technology transfer and scale-up of the Flublok process has major implications for being ready to make vaccine rapidly on a worldwide scale as a defense against pandemic influenza. The technology described does not have the same vulnerability to mutations in the egg adapted strain, and resulting loss in vaccine efficacy, faced by egg based manufacture. Copyright © 2014 Elsevier Ltd. All rights reserved.
The hot deformation behavior and microstructure evolution of HA/Mg-3Zn-0.8Zr composites for biomedical application.

Science.gov (United States)

Liu, Debao; Liu, Yichi; Zhao, Yue; Huang, Y; Chen, Minfang

2017-08-01

The hot deformation behavior of nano-sized hydroxylapatite (HA) reinforced Mg-3Zn-0.8Zr composites were performed by means of Gleeble-1500D thermal simulation machine in a temperature range of 523-673K and a strain rate range of 0.001-1s -1 , and the microstructure evolution during hot compression deformation were also investigated. The results show that the flow stress increases increasing strain rates at a constant temperature, and decreases with increasing deforming temperatures at a constant strain rate. Under the same processing conditions, the flow stresses of the 1HA/Mg-3Zn-0.8Zr specimens are higher than those of the Mg-3Zn-0.8Zr alloy specimens, and the difference is getting closer with increasing deformation temperature. The hot deformation behaviors of Mg-3Zn-0.8Zr and 1HA/Mg-3Zn-0.8Zr can be described by constitutive equation of hyperbolic sine function with the hot deformation activation energy being 124.6kJ/mol and 125.3kJ/mol, respectively. Comparing with Mg-3Zn-0.8Zr alloy, the instability region in the process map of 1HA/Mg-3Zn-0.8Zr expanded to a bigger extent at the same conditions. The optimum process conditions of 1HA/Mg-3Zn-0.8Zr composite is concluded as between the temperature window of 573-623K with a strain rate range of 0.001-0.1s -1 . A higher volume fraction and smaller grain size of dynamic recrystallization (DRX) grains was observed in 1HA/Mg-3Zn-0.8Zr specimens after the hot compression deformation compared with Mg-3Zn-0.8Zr alloy, which was ascribed to the presence of the HA particles that play an important role in particle-stimulated nucleation (PSN) mechanism and can effectively hinder the migration of interfaces. Copyright © 2017 Elsevier B.V. All rights reserved.
Terverticillate penicillia studied by direct electrospray mass spectrometric profiling of crude extracts II. Database and identification

DEFF Research Database (Denmark)

Smedsgaard, Jørn

1997-01-01

A mass spectral database was built using standard instrument software from 678 electrospray mass spectra (mass profiles) from crude fungal extracts of terverticillate taxa within the genus Penicillium. The match factors calculated from searching all the mass profiles stored in the database were...
Polycystic Ovarian Syndrome: Is It Time to Rename PCOS to HA-PODS?

Science.gov (United States)

Khadilkar, Suvarna Satish

2016-04-01

The term polycystic ovarian syndrome (PCOS) came into existence 80 years ago. Pathophysiology of PCOS remains ill understood despite extensive research in this field. It is now accepted that the manifestations of PCOS are not confined to the reproductive dysfunction, and there are endocrine-metabolic implications to PCOS with several consequences to female health. PCOS is a misnomer as ovaries do not contain epithelial cysts, but they are actually antral follicles. Moreover, the name PCOS neither reflects the hyperandrogenism which is essential for diagnosis nor the metabolic derangements. While various authors have expressed the need for change of the name, a suitable new option has not yet been established. This review aims to analyse the current understanding of pathophysiology of PCOS and addresses to the controversies associated with its diagnosis and nomenclature. The name "Hyperandrogenic Persistent Ovulatory Dysfunction Syndrome or HA-PODS" is proposed here to overcome diagnostic pitfalls of previous nomenclature. This new name will help formulate appropriate treatment and promote consistency in research as well. Further categorizations of HA-PODS are also discussed in the article.
Voreloxin is an anticancer quinolone derivative that intercalates DNA and poisons topoisomerase II.

Directory of Open Access Journals (Sweden)

Rachael E Hawtin

2010-04-01

Full Text Available Topoisomerase II is critical for DNA replication, transcription and chromosome segregation and is a well validated target of anti-neoplastic drugs including the anthracyclines and epipodophyllotoxins. However, these drugs are limited by common tumor resistance mechanisms and side-effect profiles. Novel topoisomerase II-targeting agents may benefit patients who prove resistant to currently available topoisomerase II-targeting drugs or encounter unacceptable toxicities. Voreloxin is an anticancer quinolone derivative, a chemical scaffold not used previously for cancer treatment. Voreloxin is completing Phase 2 clinical trials in acute myeloid leukemia and platinum-resistant ovarian cancer. This study defined voreloxin's anticancer mechanism of action as a critical component of rational clinical development informed by translational research.Biochemical and cell-based studies established that voreloxin intercalates DNA and poisons topoisomerase II, causing DNA double-strand breaks, G2 arrest, and apoptosis. Voreloxin is differentiated both structurally and mechanistically from other topoisomerase II poisons currently in use as chemotherapeutics. In cell-based studies, voreloxin poisoned topoisomerase II and caused dose-dependent, site-selective DNA fragmentation analogous to that of quinolone antibacterials in prokaryotes; in contrast etoposide, the nonintercalating epipodophyllotoxin topoisomerase II poison, caused extensive DNA fragmentation. Etoposide's activity was highly dependent on topoisomerase II while voreloxin and the intercalating anthracycline topoisomerase II poison, doxorubicin, had comparable dependence on this enzyme for inducing G2 arrest. Mechanistic interrogation with voreloxin analogs revealed that intercalation is required for voreloxin's activity; a nonintercalating analog did not inhibit proliferation or induce G2 arrest, while an analog with enhanced intercalation was 9.5-fold more potent.As a first-in-class anticancer
Investigation of morphology and bioactive properties of composite coating of HA/vinyl acetate on pure titanium

International Nuclear Information System (INIS)

Afshar, Abdollahe; Yousefpour, Mardali; Xiudong, Yang; Li Xudong; Yang Bangcheng; Wu Yao; Chen Jiyong; Zhang Xingdong

2006-01-01

Electrochemical co-deposition approach was expanded to prepare composite bio-ceramic coating of hydroxyapatite (HA)/polyvinyl acetate on the surface of titanium. The role is to improve the bioactive and crystallization properties. The results of XRD, XPS, SEM and TEM characterization showed that by increasing amount of vinyl acetate in the composite bio-ceramic coating before and after immersing in the simulated body fluid (SBF), an oriented growth of HA planes on the (0 0 2) direction had been observed on titanium substrate. Also significant surface morphology changes were obtained
Gene expression profiles as prognostic markers in women with ovarian cancer

DEFF Research Database (Denmark)

Jochumsen, Kirsten M; Tan, Qihua; Høgdall, Estrid V

2009-01-01

toward investigations for more individualized therapies and the use of gene expression profiles in the clinical practice. RNA from tumor tissue from 43 Danish patients with serous epithelial ovarian carcinoma (11 International Federation of Gynecology and Obstetrics [FIGO] stage I/II, 32 FIGO stage III...
ALT-II toroidal belt limiter biasing experiments on TEXTOR

International Nuclear Information System (INIS)

Doerner, R.; Boedo, J.A.; Gray, D.S.

1991-01-01

Edge electric fields have been related to H-mode-like behaviour. The experiments reported here are an attempt to control the SOL profiles by electrostatic biasing of the full toroidal-belt limiter ALT-II. The specific goals are: influencing the edge particle flows, particle removal, power deposition and the global confinement. The ALT-II pump limiter is a full toroidal belt located at 45 o below the outer midplane and consisting of eight graphite covered blades which can be independently biased. Particle scoops located behind the limiter neutralize and direct the incoming plasma into the pumping ducts. (author) 5 refs., 3 figs
Pengaruh pemberian yogurt sinbiotik tepung pisang tanduk terhadap profil lipid tikus sindrom metabolik

Directory of Open Access Journals (Sweden)

Zana Fitriana Octavia

2017-04-01

Full Text Available Background: The metabolic syndrome, which is characterized by hyperglycemia, obesity, dyslipidemia, hypertension, prothrombic and proinflamatory state, is a risk factor for cardiovascular disease and diabetes mellitus. The synbiotic yogurt of tanduk banana (Musa paradisiaca fa. corniculata flour contains lactic acid bacteria, fructooligosaccarides, flavonoids, and vitamin C which all of them play role in improving the lipid profile. Objective: The aim of this study was to prove the effect of synbiotic yogurt of tanduk banana flour on lipid profile of metabolic syndrome rats. Method: This study was an true experimental with pre-post test control group design. The subject of this study were 18 metabolic syndrome male Wistar rats divided into 3 groups,i.e control group (standart diet, intervention group I (standart diet and synbiotic yogurt of banana flour 0,009 ml/g weight/day, and intervention group II (standart diet and synbiotic yogurt of banana flour 0,018 ml/g weight/day. The intervention period was 2 weeks. Different test before and after intervention used paired t-test. The difference test between groups used One-Way ANOVA and Kruskal wallis. Results: The result showed that both of intervention group improved lipid profile significantly (p<0,05. The intervention group II showed more effective improvement of lipid profile significantly (p<0,05 than intervention group I. The intervention group II decreased triglycerides level 41,56%; total cholesterol level 41,39%; LDL-cholesterol level 57,5%; and increased HDL-cholesterol level 139,62%. Conclucion: The intervention of synbiotic yogurt of tanduk banana flour can decrease triglyceride level, total cholesterol level, LDL-cholesterol level, and increase HDL-cholesterol level.
Structure and transcription of the Helicoverpa armigera densovirus (HaDV2) genome and its expression strategy in LD652 cells.

Science.gov (United States)

Xu, Pengjun; Graham, Robert I; Wilson, Kenneth; Wu, Kongming

2017-02-07

Densoviruses (DVs) are highly pathogenic to their hosts. However, we previously reported a mutualistic DV (HaDV2). Very little was known about the characteristics of this virus, so herein we undertook a series of experiments to explore the molecular biology of HaDV2 further. Phylogenetic analysis showed that HaDV2 was similar to members of the genus Iteradensovirus. However, compared to current members of the genus Iteradensovirus, the sequence identity of HaDV2 is less than 44% at the nucleotide-level, and lower than 36, 28 and 19% at the amino-acid-level of VP, NS1 and NS2 proteins, respectively. Moreover, NS1 and NS2 proteins from HaDV2 were smaller than those from other iteradensoviruses due to their shorter N-terminal sequences. Two transcripts of about 2.2 kb coding for the NS proteins and the VP proteins were identified by Northern Blot and RACE analysis. Using specific anti-NS1 and anti-NS2 antibodies, Western Blot analysis revealed a 78 kDa and a 48 kDa protein, respectively. Finally, the localization of both NS1 and NS2 proteins within the cell nucleus was determined by using Green Fluorescent Protein (GFP) labelling. The genome organization, terminal hairpin structure, transcription and expression strategies as well as the mutualistic relationship with its host, suggested that HaDV2 was a novel member of the genus Iteradensovirus within the subfamily Densovirinae.
Behavior of lambda 2800 Mg II in stellar spectra

International Nuclear Information System (INIS)

Gurzadyan, G.A.

1975-01-01

The results of measurements of the equivalent widths of the resonance doublet of ionized magnesium lambda 2800 Mg II in the spectra of 51 relatively faint stars, up to 10/sup m/, of the spectral classes B1-K5 are presented. The observed material has been obtained by means of the space observatory ''Orion-2''. Some regularities in the behavior of lambda 2800 Mg II in stellar spectra have been revealed: wide and deep depression of the continuous spectra at 2800 A in F-G type stars; the presence of the doublet lambda 2800 Mg II in the form of weak emission in the spectra of cold stars (K2-K5); the presence both of the multiplet lambda 3080 Ti II and the doublet lambda 2800 Mg II simultaneously either in emission--in the late-type stars--or in absorption in earlier types; the existence of combined profiles of lambda 2800 Mg II, i.e., a wide absorption line with a weak emission in the center, in stars of the transitional class (G5-K0), etc. A well-defined empirical relationship between the equivalent width of lambda 2800 Mg II and the spectral class of the star has been established (Fig. 8). (U.S.)
Complexes of cobalt(II), nickel(II), copper(II), zinc(II), cadmium(II) and dioxouranium(II) with thiophene-2-aldehydethiosemicarbazone

International Nuclear Information System (INIS)

Singh, Balwan; Misra, Harihar

1986-01-01

Metal complexes of thiosemicarbazides have been known for their pharmacological applications. Significant antitubercular, fungicidal and antiviral activities have been reported for thiosemicarbazides and their derivatives. The present study describes the systhesis and characterisation of complexes of Co II , Cu II , Zn II ,Cd II and UO II with thiosemicarbazone obtained by condensing thiophene-2-aldehyde with thiosemicarbazide. 17 refs., 2 tables. (author)
Euroveokid soosivad Venemaal käies üha enam Via Hanseaticat / Madis Aesma

Index Scriptorium Estoniae

Aesma, Madis

2005-01-01

Ajal. Postimees lüh. Pärast Balti riikide ühinemist Euroopa Liiduga kasutavad välismaa veoautojuhid üha enam Euroopast Venemaale sõitmiseks Valga-Tartu-Jõhvi-Narva maanteed, mis moodustab Via Hanseatica ehk Hansatee Eesti osa. Lisa: Via Hanseatica. Kaart: Visa Hanseatica
Áreas metropolitanas:¿qué ha cambiado?: La experiencia de la Caracas Metropolitana

Directory of Open Access Journals (Sweden)

Sonia Barrios

2001-05-01

Full Text Available La nueva economía de la información y del conocimiento ha tenido tres decisivas implicaciones de orden territorial. Primero, ha acelerado el proceso de aglomeración urbana a escala planetaria. Segundo, ha provocado fuertes alteraciones en las redes urbanas de ámbito mundial, regional y nacional. Y tercero, ha impulsado la reestructuración interna de las áreas metropolitanas que se habían conformado durante la era industrial. Todo parece anticipar, por lo tanto, polarizaciones y fragmentaciones territoriales todavía más acentuadas que las conocidas en un pasado cercano. Tomando como referencia el caso de Caracas, se trata de averiguar en qué medida el desarrollo reciente de esta metrópoli sigue las grandes tendencias territoriales antes anotadas; y qué esfuerzos se están haciendo para ajustar sus instancias locales a las nuevas realidades urbanas. Como un recurso adicional, en distintos momentos se establecen comparaciones con Barcelona (España, considerada un ejemplo emblemático de las metrópolis de la era de la informaciónThe developments of new informational and knowledge-based economies have had three decisive implications at the territorial level. First, it has accelerated the urban agglomeration processes worldwide. Second, it has brought about substantial alterations in the existing global, regional and national urban networks. Finally, it has triggered the internal restructuring of former industrial-based metropolitan areas. All this seems to anticipate that territorial polarization and fragmentation will reach higher levels than those we have observed in the recent past. Taking the case of Caracas as a reference point, the intention is to find out to what extent the recent development of this metropolis follows the main territorial trends mentioned above. Interest is also placed in finding what steps are being taken in order to adjust its local levels of government to the new urban realities. In addition, comparisons are

(+)-Nootkatone inhibits tumor necrosis factor α/interferon γ-induced production of chemokines in HaCaT cells

Energy Technology Data Exchange (ETDEWEB)

Choi, Hyeon-Jae; Lee, Jin-Hwee [College of Pharmacy, Ajou University, Suwon 443-749 (Korea, Republic of); Jung, Yi-Sook, E-mail: yisjung@ajou.ac.kr [College of Pharmacy, Ajou University, Suwon 443-749 (Korea, Republic of); Research Institute of Pharmaceutical Sciences and Technology, Ajou University, Suwon 443-749 (Korea, Republic of)

2014-05-02

Highlights: • (+)-Nootkatone inhibits TNF-α/IFN-γ-induced TARC and MDC expression in HaCaT cells. • PKCζ, p38 MAPK, or NF-κB mediate TNF-α/IFN-γ-induced TARC and MDC expression. • (+)-Nootkatone inhibits TNF-α/IFN-γ-induced activation of PKCζ, p38 MAPK, or NF-κB. • (+)-Nootkatone suppresses chemokine expression by inhibiting of PKCζ and p38 pathways. - Abstract: Chemokines are important mediators of cell migration, and thymus and activation-regulated chemokine (TARC/CCL17) and macrophage-derived chemokine (MDC/CCL22) are well-known typical inflammatory chemokines involved in atopic dermatitis (AD). (+)-Nootkatone is the major component of Cyperus rotundus. (+)-Nootkatone has antiallergic, anti-inflammatory, and antiplatelet activities. The purpose of this study was to investigate the effect of (+)-nootkatone on tumor necrosis factor α (TNF-α)/interferon γ (IFN-γ)-induced expression of Th2 chemokines in HaCaT cells. We found that (+)-nootkatone inhibited the TNF-α/IFN-γ-induced expression of TARC/CCL17 and MDC/CCL22 mRNA in HaCaT cells. It also significantly inhibited TNF-α/IFN-γ-induced activation of nuclear factor kappa B (NF-κB), p38 mitogen-activated protein kinase (MAPK), and protein kinase Cζ (PKCζ). Furthermore, we showed that PKCζ and p38 MAPK contributed to the inhibition of TNF-α/IFN-γ-induced TARC/CCL17 and MDC/CCL22 expression by blocking IκBα degradation in HaCaT cells. Taken together, these results suggest that (+)-nootkatone may suppress TNF-α/IFN-γ-induced TARC/CCL17 and MDC/CCL22 expression in HaCaT cells by inhibiting of PKCζ and p38 MAPK signaling pathways that lead to activation of NF-κB. We propose that (+)-nootkatone may be a useful therapeutic candidate for inflammatory skin diseases such as AD.
Raman lidar water vapor profiling over Warsaw, Poland

Science.gov (United States)

Stachlewska, Iwona S.; Costa-Surós, Montserrat; Althausen, Dietrich

2017-09-01

Water vapor mixing ratio and relative humidity profiles were derived from the multi-wavelength Raman PollyXT lidar at the EARLINET site in Warsaw, using the Rayleigh molecular extinction calculation based on atmospheric temperature and pressure from three different sources: i) the standard atmosphere US 62, ii) the Global Data Assimilation System (GDAS) model output, and iii) the WMO 12374 radiosoundings launched at Legionowo. With each method, 136 midnight relative humidity profiles were obtained for lidar observations from July 2013 to August 2015. Comparisons of these profiles showed in favor of the latter method (iii), but it also indicated that the other two data sources could replace it, if necessary. Such use was demonstrated for an automated retrieval of water vapor mixing ratio from dusk until dawn on 19/20 March 2015; a case study related to an advection of biomass burning aerosol from forest fires over Ukraine. Additionally, an algorithm that applies thresholds to the radiosounding relative humidity profiles to estimate macro-physical cloud vertical structure was used for the first time on the Raman lidar relative humidity profiles. The results, based on a subset of 66 profiles, indicate that below 6 km cloud bases/tops can be successfully obtained in 53% and 76% cases from lidar and radiosounding profiles, respectively. Finally, a contribution of the lidar derived mean relative humidity to cloudy conditions within the range of 0.8 to 6.2 km, in comparison to clear-sky conditions, was estimated.
Profiles of Automotive Suppliers Industries--Engineered Mechanical Components and Systems : Volume II, Appendices.

Science.gov (United States)

1981-09-01

The profile describes and analyzes that segment of the automotive supplier industry which provides engineered mechanical components/assemblies/systems to the prime auto manufacturers. It presents an overview of the role and structure of this industry...
Stokes profile analysis and vector magnetic fields. III. Extended temperature minima of sunspot umbrae as inferred from Stokes profiles of Mg I 4571 A

International Nuclear Information System (INIS)

Lites, B.W.; Skumanich, A.; Rees, D.E.; Murphy, G.A.; Carlsson, M.; Sydney Univ., Australia; Oslo Universitetet, Norway)

1987-01-01

Observed Stokes profiles of Mg I 4571 A are analyzed as a diagnostic of the magnetic field and thermal structure at the temperature minimum of sunspot umbrae. Multilevel non-LTE transfer calculations of the Mg I-II-III excitation and ionization balance in model umbral atmospheres show: (1) Mg I to be far less ionized in sunspot umbrae than in the quiet sun, leading to greatly enhanced opacity in 4571 A, and (2) LTE excitation of 4571 A. Existing umbral models predict emission cores of the Stokes I profile due to the chromospheric temperature rise. This feature is not present in observed umbral profiles. Moreover, such an emission reversal causes similar anomalous features in the Stokes Q, U, V profiles, which are also not observed. Umbral atmospheres with extended temperature minima are suggested. Implications for chromospheric heating mechanisms and the utility of this line for solar vector magnetic field measurements are discussed. 35 references
Reactive Fe(II) layers in deep-sea sediments

Science.gov (United States)

König, Iris; Haeckel, Matthias; Drodt, Matthias; Suess, Erwin; Trautwein, Alfred X.

1999-05-01

The percentage of the structural Fe(II) in clay minerals that is readily oxidized to Fe(III) upon contact with atmospheric oxygen was determined across the downcore tan-green color change in Peru Basin sediments. This latent fraction of reactive Fe(II) was only found in the green strata, where it proved to be large enough to constitute a deep reaction layer with respect to the pore water O 2 and NO 3-. Large variations were detected in the proportion of the reactive Fe(II) concentration to the organic matter content along core profiles. Hence, the commonly observed tan-green color change in marine sediments marks the top of a reactive Fe(II) layer, which may represent the major barrier to the movement of oxidation fronts in pelagic subsurface sediments. This is also demonstrated by numerical model simulations. The findings imply that geochemical barriers to pore water oxidation fronts form diagenetically in the sea floor wherever the stage of iron reduction is reached, provided that the sediments contain a significant amount of structural iron in clay minerals.
Radioactive cDNA microarray (II): Gene expression profiling of antidepressant treatment by human cDNA microarray

Energy Technology Data Exchange (ETDEWEB)

Lee, Ji Hye; Kang, Rhee Hun; Ham, Byung Joo; Lee, Min Su; Shin, Kyung Ho; Choe, Jae Gol; Kim, Meyoung Kon [College of Medicine, Univ. of Korea, Seoul (Korea, Republic of)

2003-07-01

Major depressive disorder is a prevalent psychiatric disorder in primary care, associated with impaired patient functioning and well-being. Fluoxetine is a selective serotonin-reuptake inhibitors (SSRIs) and is a commonly prescribed antidepressant compound. Its action is primarily attributed to selective inhibition of the reuptake of serotonin (5-hydroxytryptamine) in the central nervous system. Objectives ; the aims of this study were two-fold: (1) to determine the usefulness for investigation of the transcription profiles in depression patients, and (2) to assess the differences in gene expression profiles between positive response group and negative response groups by fluoxetine treatment. This study included 53 patients with major depression (26 in positive response group with antidepressant treatment, 27 in negative response group with antidepressant treatment), and 53 healthy controls. To examine the difference of gene expression profile in depression patients, radioactive complementary DNA microarrays were used to evaluate changes in the expression of 1,152 genes in total. Using 33p-labeled probes, this method provided highly sensitive gene expression profiles including brain receptors, drug metabolism, and cellular signaling. Gene transcription profiles were classified into several categories in accordance with the antidepressant gene-regulation. The gene profiles were significantly up-(22 genes) and down-(16 genes) regulated in the positive response group when compared to the control group. Also, in the negative response group, 35 genes were up-regulated and 8 genes were down-regulated when compared to the control group. Consequently, we demonstrated that radioactive human cDNA microarray is highly likely to be an efficient technology for evaluating the gene regulation of antidepressants, such as selective serotonin-reuptake inhibitors (SSRIs), by using high-throughput biotechnology.
Radioactive cDNA microarray (II): Gene expression profiling of antidepressant treatment by human cDNA microarray

International Nuclear Information System (INIS)

Lee, Ji Hye; Kang, Rhee Hun; Ham, Byung Joo; Lee, Min Su; Shin, Kyung Ho; Choe, Jae Gol; Kim, Meyoung Kon

2003-01-01

Major depressive disorder is a prevalent psychiatric disorder in primary care, associated with impaired patient functioning and well-being. Fluoxetine is a selective serotonin-reuptake inhibitors (SSRIs) and is a commonly prescribed antidepressant compound. Its action is primarily attributed to selective inhibition of the reuptake of serotonin (5-hydroxytryptamine) in the central nervous system. Objectives ; the aims of this study were two-fold: (1) to determine the usefulness for investigation of the transcription profiles in depression patients, and (2) to assess the differences in gene expression profiles between positive response group and negative response groups by fluoxetine treatment. This study included 53 patients with major depression (26 in positive response group with antidepressant treatment, 27 in negative response group with antidepressant treatment), and 53 healthy controls. To examine the difference of gene expression profile in depression patients, radioactive complementary DNA microarrays were used to evaluate changes in the expression of 1,152 genes in total. Using 33p-labeled probes, this method provided highly sensitive gene expression profiles including brain receptors, drug metabolism, and cellular signaling. Gene transcription profiles were classified into several categories in accordance with the antidepressant gene-regulation. The gene profiles were significantly up-(22 genes) and down-(16 genes) regulated in the positive response group when compared to the control group. Also, in the negative response group, 35 genes were up-regulated and 8 genes were down-regulated when compared to the control group. Consequently, we demonstrated that radioactive human cDNA microarray is highly likely to be an efficient technology for evaluating the gene regulation of antidepressants, such as selective serotonin-reuptake inhibitors (SSRIs), by using high-throughput biotechnology
Extraction of hyaluronic acid (HA) from rooster comb and characterization using flow field-flow fractionation (FlFFF) coupled with multiangle light scattering (MALS).

Science.gov (United States)

Kang, Dong Young; Kim, Won-Suk; Heo, In Sook; Park, Young Hun; Lee, Seungho

2010-11-01

Hyaluronic acid (HA) was extracted in a relatively large scale from rooster comb using a method similar to that reported previously. The extraction method was modified to simplify and to reduce time and cost in order to accommodate a large-scale extraction. Five hundred grams of frozen rooster combs yielded about 500 mg of dried HA. Extracted HA was characterized using asymmetrical flow field-flow fractionation (AsFlFFF) coupled online to a multiangle light scattering detector and a refractive index detector to determine the molecular size, molecular weight (MW) distribution, and molecular conformation of HA. For characterization of HA, AsFlFFF was operated by a simplified two-step procedure, instead of the conventional three-step procedure, where the first two steps (sample loading and focusing) were combined into one to avoid the adsorption of viscous HA onto the channel membrane. The simplified two-step AsFlFFF yielded reasonably good separations of HA molecules based on their MWs. The weight average MW (M(w) ) and the average root-mean-square (RMS) radius of HA extracted from rooster comb were 1.20×10(6) and 94.7 nm, respectively. When the sample solution was filtered through a 0.45 μm disposable syringe filter, they were reduced down to 3.8×10(5) and 50.1 nm, respectively. Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Antinociceptive action of oxytocin involves inhibition of potassium channel currents in lamina II neurons of the rat spinal cord

Directory of Open Access Journals (Sweden)

Darbon Pascal

2009-11-01

Full Text Available Abstract Background Growing evidence in the literature shows that oxytocin (OT has a strong spinal anti-nociceptive action. Oxytocinergic axons originating from a subpopulation of paraventricular hypothalamic neurons establish synaptic contacts with lamina II interneurons but little is known about the functional role of OT with respect to neuronal firing and excitability. Results Using the patch-clamp technique, we have recorded lamina II interneurons in acute transverse lumbar spinal cord slices of rats (15 to 30 days old and analyzed the OT effects on action potential firing ability. In the current clamp mode, we found that bath application of a selective OT-receptor agonist (TGOT reduced firing in the majority of lamina II interneurons exhibiting a bursting firing profile, but never in those exhibiting a single spike discharge upon depolarization. Interestingly, OT-induced reduction in spike frequency and increase of firing threshold were often observed, leading to a conversion of the firing profile from repetitive and delayed profiles into phasic ones and sometimes further into single spike profile. The observed effects following OT-receptor activation were completely abolished when the OT-receptor agonist was co-applied with a selective OT-receptor antagonist. In current and voltage clamp modes, we show that these changes in firing are strongly controlled by voltage-gated potassium currents. More precisely, transient IA currents and delayed-rectifier currents were reduced in amplitude and transient IA current was predominantly inactivated after OT bath application. Conclusion This effect of OT on the firing profile of lamina II neurons is in good agreement with the antinociceptive and analgesic properties of OT described in vivo.
Dissolved inorganic carbon, pH, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, Coulometer for DIC measurement and other instruments from the Ryofu Maru II in the North Pacific Ocean from 2003-06-20 to 2003-07-13 (NODC Accession 0112281)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0112281 includes biological, chemical, discrete sample, physical and profile data collected from Ryofu Maru II in the North Pacific Ocean from...
Dissolved inorganic carbon, pH, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, Coulometer for DIC measurement and other instruments from the Ryofu Maru II in the North Pacific Ocean from 2004-06-08 to 2004-07-03 (NODC Accession 0112284)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0112284 includes biological, chemical, discrete sample, physical and profile data collected from Ryofu Maru II in the North Pacific Ocean from...
Dissolved inorganic carbon, pH, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, Coulometer for DIC measurement and other instruments from the Ryofu Maru II in the North Pacific Ocean from 2003-04-25 to 2003-05-12 (NODC Accession 0112280)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0112280 includes biological, chemical, discrete sample, physical and profile data collected from Ryofu Maru II in the North Pacific Ocean from...
Discrepancies between conformational distributions of a polyalanine peptide in solution obtained from molecular dynamics force fields and amide I' band profiles.

Science.gov (United States)

Verbaro, Daniel; Ghosh, Indrajit; Nau, Werner M; Schweitzer-Stenner, Reinhard

2010-12-30

Structural preferences in the unfolded state of peptides determined by molecular dynamics still contradict experimental data. A remedy in this regard has been suggested by MD simulations with an optimized Amber force field ff03* ( Best, R. Hummer, G. J. Phys. Chem. B 2009 , 113 , 9004 - 9015 ). The simulations yielded a statistical coil distribution for alanine which is at variance with recent experimental results. To check the validity of this distribution, we investigated the peptide H-A(5)W-OH, which with the exception of the additional terminal tryptophan is analogous to the peptide used to optimize the force fields ff03*. Electronic circular dichroism, vibrational circular dichroism, and infrared spectroscopy as well as J-coupling constants obtained from NMR experiments were used to derive the peptide's conformational ensemble. Additionally, Förster resonance energy transfer between the terminal chromophores of the fluorescently labeled peptide analogue H-Dbo-A(5)W-OH was used to determine its average length, from which the end-to-end distance of the unlabeled peptide was estimated. Qualitatively, the experimental (3)J(H(N),C(α)), VCD, and ECD indicated a preference of alanine for polyproline II-like conformations. The experimental (3)J(H(N),C(α)) for A(5)W closely resembles the constants obtained for A(5). In order to quantitatively relate the conformational distribution of A(5) obtained with the optimized AMBER ff03* force field to experimental data, the former was used to derive a distribution function which expressed the conformational ensemble as a mixture of polyproline II, β-strand, helical, and turn conformations. This model was found to satisfactorily reproduce all experimental J-coupling constants. We employed the model to calculate the amide I' profiles of the IR and vibrational circular dichroism spectrum of A(5)W, as well as the distance between the two terminal peptide carbonyls. This led to an underestimated negative VCD couplet and an
Comparison of Solar Fine Structure Observed Simultaneously in Lyα and Mg II h

Science.gov (United States)

Schmit, D.; Sukhorukov, A. V.; De Pontieu, B.; Leenaarts, J.; Bethge, C.; Winebarger, A.; Auchère, F.; Bando, T.; Ishikawa, R.; Kano, R.; Kobayashi, K.; Narukage, N.; Trujillo Bueno, J.

2017-10-01

The Chromospheric Lyman Alpha Spectropolarimeter (CLASP) observed the Sun in H I Lyα during a suborbital rocket flight on 2015 September 3. The Interface Region Imaging Telescope (IRIS) coordinated with the CLASP observations and recorded nearly simultaneous and co-spatial observations in the Mg II h and k lines. The Mg II h and Lyα lines are important transitions, energetically and diagnostically, in the chromosphere. The canonical solar atmosphere model predicts that these lines form in close proximity to each other and so we expect that the line profiles will exhibit similar variability. In this analysis, we present these coordinated observations and discuss how the two profiles compare over a region of quiet Sun at viewing angles that approach the limb. In addition to the observations, we synthesize both line profiles using a 3D radiation-MHD simulation. In the observations, we find that the peak width and the peak intensities are well correlated between the lines. For the simulation, we do not find the same relationship. We have attempted to mitigate the instrumental differences between IRIS and CLASP and to reproduce the instrumental factors in the synthetic profiles. The model indicates that formation heights of the lines differ in a somewhat regular fashion related to magnetic geometry. This variation explains to some degree the lack of correlation, observed and synthesized, between Mg II and Lyα. Our analysis will aid in the definition of future observatories that aim to link dynamics in the chromosphere and transition region.
Comparison of Solar Fine Structure Observed Simultaneously in Ly α and Mg ii h

Energy Technology Data Exchange (ETDEWEB)

Schmit, D. [Bay Area Environmental Research Institute, 625 2nd Street, Suite 209, Petaluma, CA 94952 (United States); Sukhorukov, A. V.; Leenaarts, J. [Institute for Theoretical Astrophysics, University of Oslo, P.O. Box 1029, Blindern NO-0315 Oslo (Norway); De Pontieu, B. [Lockheed Martin Solar and Astrophysics Laboratory, Building 252, 3176 Porter Drive, Palo Alto, CA 94304 (United States); Bethge, C.; Winebarger, A.; Kobayashi, K. [NASA Marshall Space Flight Center, ZP 13, Huntsville, AL 35812 (United States); Auchère, F. [Institut d’Astrophysique Spatiale, CNRS/Univ. Paris-Sud 11, Bâtiment 121, F-91405 Orsay (France); Bando, T.; Kano, R.; Narukage, N. [National Astronomical Observatory of Japan, National Institutes of Natural Sciences, 2-21-1 Osawa, Mitaka, Tokyo 181-8588 (Japan); Ishikawa, R. [Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency, 3-1-1 Yoshinodai, Chuo-ku, Sagamihara, Kanagawa 252-5210 (Japan); Bueno, J. Trujillo [Instituto de Astrofísica de Canarias, E-38205 La Laguna, Tenerife (Spain)

2017-10-01

The Chromospheric Lyman Alpha Spectropolarimeter (CLASP) observed the Sun in H i Ly α during a suborbital rocket flight on 2015 September 3. The Interface Region Imaging Telescope ( IRIS ) coordinated with the CLASP observations and recorded nearly simultaneous and co-spatial observations in the Mg ii h and k lines. The Mg ii h and Ly α lines are important transitions, energetically and diagnostically, in the chromosphere. The canonical solar atmosphere model predicts that these lines form in close proximity to each other and so we expect that the line profiles will exhibit similar variability. In this analysis, we present these coordinated observations and discuss how the two profiles compare over a region of quiet Sun at viewing angles that approach the limb. In addition to the observations, we synthesize both line profiles using a 3D radiation-MHD simulation. In the observations, we find that the peak width and the peak intensities are well correlated between the lines. For the simulation, we do not find the same relationship. We have attempted to mitigate the instrumental differences between IRIS and CLASP and to reproduce the instrumental factors in the synthetic profiles. The model indicates that formation heights of the lines differ in a somewhat regular fashion related to magnetic geometry. This variation explains to some degree the lack of correlation, observed and synthesized, between Mg ii and Ly α . Our analysis will aid in the definition of future observatories that aim to link dynamics in the chromosphere and transition region.
Injection of an antibody against a p21 c-Ha-ras protein inhibits cleavage in axolotl eggs.

OpenAIRE

Baltus, E; Hanocq-Quertier, J; Hanocq, F; Brachet, J

1988-01-01

The presence of a ras protein was demonstrated in cleaving axolotl eggs by selective immunoprecipitation with a polyclonal antibody against a peptide encoded by the c-Ha-ras oncogene, cellular homolog of the v-Ha-ras oncogene of Harvey rat sarcoma virus. Injection of this antibody into axolotl oocytes subjected to progesterone treatment does not prevent meiotic maturation. Injection of the same antibody into a blastomere of axolotl eggs at the 2- or 4-cell stage causes cleavage arrest in the ...
Mn(II), Zn(II) and VO(II) Schiff

Indian Academy of Sciences (India)

Home; Journals; Journal of Chemical Sciences; Volume 113; Issue 3. Synthesis and characterisation of Cu(II), Ni(II), Mn(II), Zn(II) and VO(II) Schiff base complexes derived from o-phenylenediamine and acetoacetanilide. N Raman Y Pitchaikani Raja A Kulandaisamy. Inorganic Volume 113 Issue 3 June 2001 pp 183-189 ...
Dihydrotestosterone induces SREBP-1 expression and lipogenesis through the phosphoinositide 3-kinase/Akt pathway in HaCaT cells

Directory of Open Access Journals (Sweden)

Zhou Bing-rong

2012-11-01

Full Text Available Abstract Background The purpose of this study was to investigate the effects and mechanisms of dihydrotestosterone (DHT-induced expression of sterol regulatory element binding protein-1 (SREBP-1, and the synthesis and secretion of lipids, in HaCaT cells. HaCaT cells were treated with DHT and either the phosphoinositide 3-kinase inhibitor LY294002 or the extracellular-signal-regulated kinase (ERK inhibitor PD98059. Real time-PCR, Western blot, Oil Red staining and flow cytometry were employed to examine the mRNA and protein expressions of SREBP-1, the gene transcription of lipid synthesis, and lipid secretion in HaCaT cells. Findings We found that DHT upregulated mRNA and protein expressions of SREBP-1. DHT also significantly upregulated the transcription of lipid synthesis-related genes and increased lipid secretion, which can be inhibited by the addition of LY294002. Conclusions Collectively, these results indicate that DHT induces SREBP-1 expression and lipogenesis in HaCaT cells via activation of the phosphoinositide 3-kinase/Akt Pathway.
Cephalometric assessment of lips in skeletal class ii patients by steiner's line

International Nuclear Information System (INIS)

Bokhari, F.; Amin, F.; Asad, S.

2013-01-01

Introduction: Steiner's (S) Line has been used as reference line to assess anteroposterior position of lips cephalometrically and has been an effective diagnostic aid in this era of soft tissue paradigm. Norms for Sline has been established for different populations and it has been used widely to assess treatment outcomes in Skeletal Class II malocclusion, however anteroposterior position of lips and determinants of lip position in Skeletal Class II has not been explored. Study Design: This Prospective study was aimed to find out the anteroposterior position of lips on cephalograph using S-line in patients with retrognathic pro-file and to establish correlation between determinants of lip prominence. Data was collected using nonprobability convenience sampling technique following the selection criteria. Materials and Methods: The study was conducted on 65 subjects, with retrognathic profile as judged by orthodontists in consensus and confirmed by lateral cephalogram (ANB > 4 degree). S-Line was drawn on lateral cephalograph to assess the prominence of upper Lip and lower lip. SPSS 17.0 was used for statistical evaluation. Results: Antero-posterior position of upper and lower lip in patients with retrognathic profile with reference to S-line was 1.96 +- 2.6 mm and 3.09 +- 3.16 mm respectively. Moreover it was found that statistically significant correlation existed between lower lip prominence as assessed by S-line and upper lip prominence using the same reference line (r = 0.411), Lower incisor inclination (r = 0.535) and Skeletal Class II as assessed by ANB angle (r = 0.27). Upper lip prominence as assessed S-line was found to be statistically significantly correlated with lower incisor inclination and lower lip prominence. Discussion: Results were compliant with the previous studies.Conclusion: In the present study both upper and lower lips were more prominent in Skeletal Class II patients as compared to Steiner's norms for skeletal class I. (author)
CRED Shallow CTD Profiles; French Frigate Shoals, Northwestern Hawaiian Islands (Papahanaumokuakea Marine National Monument); Cruise: HA1007, Data Date Range: 20100908-20100910 (NODC Accession 0039382).

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — CRED shallow Conductivity-Temperature-Depth (CTD) casts are vertical profiles (max 30 meter depth, downcast only) of temperature, conductivity and pressure. Data are...

Bâtir un avenir pour Haïti grâce aux études supérieures | CRDI ...

International Development Research Centre (IDRC) Digital Library (Canada)

9 mai 2017 ... Image. Un membre du Groupe de Réflexion et d'Action pour une Haïti nouvelle. CRDI. Le tremblement de terre d'une magnitude de 7,0 qui a frappé Haïti en janvier 2010 a touché particulièrement durement les universités de ce pays. Les secousses sismiques ont détruit des immeubles, et quelque 100 000 ...
Combination of cupric ion with hydroxylamine and hydrogen peroxide for the control of bacterial biofilms on RO membranes.

Science.gov (United States)

Lee, Hye-Jin; Kim, Hyung-Eun; Lee, Changha

2017-03-01

Combinations of Cu(II) with hydroxylamine (HA) and hydrogen peroxide (H 2 O 2 ) (i.e., Cu(II)/HA, Cu(II)/H 2 O 2 , and Cu(II)/HA/H 2 O 2 systems) were investigated for the control of P. aeruginosa biofilms on reverse osmosis (RO) membranes. These Cu(II)-based disinfection systems effectively inactivated P. aeruginosa cells, exhibiting different behaviors depending on the state of bacterial cells (planktonic or biofilm) and the condition of biofilm growth and treatment (normal or pressurized condition). The Cu(II)/HA and Cu(II)/HA/H 2 O 2 systems were the most effective reagents for the inactivation of planktonic cells. However, these systems were not effective in inactivating cells in biofilms on the RO membranes possibly due to the interactions of Cu(I) with extracellular polymeric substances (EPS), where biofilms were grown and treated in center for disease control (CDC) reactors. Different from the results using CDC reactors, in a pressurized cross-flow RO filtration unit, the Cu(II)/HA/H 2 O 2 treatment significantly inactivated biofilm cells formed on the RO membranes, successfully recovering the permeate flux reduced by the biofouling. The pretreatment of feed solutions by Cu(II)/HA and Cu(II)/HA/H 2 O 2 systems (applied before the biofilm formation) effectively mitigated the permeate flux decline by preventing the biofilm growth on the RO membranes. Copyright © 2016 Elsevier Ltd. All rights reserved.
Supporting Timely Humanitarian Assistance/Disaster Relief (HA/DR) Decisions Through Geospatial Intelligence (GEOINT) and Geographical Information Systems (GIS) Tools

Science.gov (United States)

2014-05-22

APPENDIX D: JUBA HA/DR COP SHARED VIA A GOOGLE EARTH ® KML FILE ........... 60 APPENDIX E: GEOINT REFERENCE AIDS...2003 and 2012................. 51 Figure 13. JTF-Haiti (27 Jan 10): DPs, IDP groups, flood and landslide risks. ............................. 57...Figure 14. Juba HA/DR COP displayed in Google Earth ® ........................................................... 60 Figure 15. Overview of GEOINT data
The differences in temperament–character traits, suicide attempts, impulsivity, and functionality levels of patients with bipolar disorder I and II

Directory of Open Access Journals (Sweden)

Izci F

2016-01-01

Full Text Available Filiz Izci,1 Ebru Kanmaz Findikli,2 Serkan Zincir,3 Selma Bozkurt Zincir,4 Merve Iris Koc4 1Department of Psychiatry, School of Medicine, Istanbul Bilim University, Istanbul, 2Department of Psychiatry, School of Medicine, Kahramanmaras Sutcu Imam University, Kahramanmaras, 3Department of Psychiatry, Kocaeli Gölcük Military Hospital, Kocaeli, 4Department of Psychiatry, Erenköy Training and Research Hospital for Psychiatric and Neurological Disorders, Istanbul, Turkey Background: The primary aim of this study was to compare the differences in temperament-character traits, suicide attempts, impulsivity, and functionality levels of patients with bipolar disorder I (BD-I and bipolar disorder II (BD-II.Methods: Fifty-two BD-I patients and 49 BD-II patients admitted to Erenköy Mental and Neurological Disease Training and Research Hospital psychiatry clinic and fifty age- and sex-matched healthy control subjects were enrolled in this study. A structured clinical interview for Diagnostic and Statistical Manual of Mental Disorders, 4th Edition Axis I Disorders, Temperament and Character Inventory, Barrett Impulsiveness Scale-11 (BIS-11, Hamilton Depression Inventory Scale, Young Mania Rating Scale, and Bipolar Disorder Functioning Questionnaire (BDFQ were administered to patients and to control group.Results: No statistically significant difference in sociodemographic features existed between the patient and control groups (P>0.05. Thirty-eight subjects (37.62% in the patient group had a suicide attempt. Twenty-three of these subjects (60.52% had BD-I, and 15 of these subjects (39.47% had BD-II. Suicide attempt rates in BD-I and II patients were 60.52% and 39.47%, respectively (P<0.05. Comparison of BD-I and II patients with healthy control subjects revealed that cooperativeness (C, self-directedness (Sdi, and self-transcendence (ST scores were lower and novelty seeking (NS1 and NS2, harm avoidance (HA4, and reward dependence (RD2 subscale scores
Production of light fragments in hA collisions at high energies

International Nuclear Information System (INIS)

Braun, M.A.; Vechernin, V.V.

1988-12-01

Production of fast relativistic light fragments in hA collisions at high energies is considered. Direct coalescence of produced nucleons into fragments is shown to be the main mechanism for fragment production. The influence of the nuclear field is small and is not described by the well-known Butler-Pearson formulas. The coalescence coefficient strongly depends on the angle and on the behaviour of the fragment wave function at small internucleon distances. (author). 14 refs, 7 figs
Rapid Genome-wide Recruitment of RNA Polymerase II Drives Transcription, Splicing, and Translation Events during T Cell Responses

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Kathrin Davari

2017-04-01

Full Text Available Summary: Activation of immune cells results in rapid functional changes, but how such fast changes are accomplished remains enigmatic. By combining time courses of 4sU-seq, RNA-seq, ribosome profiling (RP, and RNA polymerase II (RNA Pol II ChIP-seq during T cell activation, we illustrate genome-wide temporal dynamics for ∼10,000 genes. This approach reveals not only immediate-early and posttranscriptionally regulated genes but also coupled changes in transcription and translation for >90% of genes. Recruitment, rather than release of paused RNA Pol II, primarily mediates transcriptional changes. This coincides with a genome-wide temporary slowdown in cotranscriptional splicing, even for polyadenylated mRNAs that are localized at the chromatin. Subsequent splicing optimization correlates with increasing Ser-2 phosphorylation of the RNA Pol II carboxy-terminal domain (CTD and activation of the positive transcription elongation factor (pTEFb. Thus, rapid de novo recruitment of RNA Pol II dictates the course of events during T cell activation, particularly transcription, splicing, and consequently translation. : Davari et al. visualize global changes in RNA Pol II binding, transcription, splicing, and translation. T cells change their functional program by rapid de novo recruitment of RNA Pol II and coupled changes in transcription and translation. This coincides with fluctuations in RNA Pol II phosphorylation and a temporary reduction in cotranscriptional splicing. Keywords: RNA Pol II, cotranscriptional splicing, T cell activation, ribosome profiling, 4sU, H3K36, Ser-5 RNA Pol II, Ser-2 RNA Pol II, immune response, immediate-early genes
Determination of Ca/P molar ratio in hydroxyapatite (HA) by X-ray fluorescence technique

International Nuclear Information System (INIS)

Scapin, Marcos A.; Guilhen, Sabine N.; Cotrim, Marycel E.B.; Pires, Maria Ap. F.

2015-01-01

Hydroxyapatite (HA) is a mineral composed of calcium phosphate employed for endodontics, restorative dentistry and other applications in orthopedics and prosthesis. Additionally, this biomaterial is an inexpensive but efficient adsorbent for the removal of heavy metals and other unwanted species of contaminated liquid effluents. This is especially interesting when low-cost effective remediation is required. A Ca / P molar ratio of 1.667 is consistent with the theoretical Ca / P ratio for calcium hydroxyapatite with a compositional formula of Ca 10 (PO 4 ) 6 (OH) 2 , which properties are well discussed in the literature. The aim of this work was to implement and validate a methodology for simultaneous determination of major and minor constituents in the hydroxyapatite (HA) as well as providing the Ca / P molar ratio. To accomplish these achievements, wavelength dispersive X-ray fluorescence spectroscopy (WDXRF) was applied. This is a non-destructive technique that requires no chemical treatment, enabling fast chemical analysis in a wide variety of samples, with no hazardous waste being generated as a result of the process of determination. A standard reference material from NIST (SRM 1400 – Bone Ash) was used to validate the methodology for the determination of magnesium, phosphorus, potassium, calcium, iron, zinc, strontium and the Ca / P ratio in HA samples by WDXRF. The Z-score test was applied as a statistical tool and showed that the calculated values were of less than 1.8 for all the measured analytes. (author)
Determination of Ca/P molar ratio in hydroxyapatite (HA) by X-ray fluorescence technique

Energy Technology Data Exchange (ETDEWEB)

Scapin, Marcos A.; Guilhen, Sabine N.; Cotrim, Marycel E.B.; Pires, Maria Ap. F., E-mail: mascapin@usp.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

2015-07-01

Hydroxyapatite (HA) is a mineral composed of calcium phosphate employed for endodontics, restorative dentistry and other applications in orthopedics and prosthesis. Additionally, this biomaterial is an inexpensive but efficient adsorbent for the removal of heavy metals and other unwanted species of contaminated liquid effluents. This is especially interesting when low-cost effective remediation is required. A Ca / P molar ratio of 1.667 is consistent with the theoretical Ca / P ratio for calcium hydroxyapatite with a compositional formula of Ca{sub 10}(PO{sub 4}){sub 6}(OH){sub 2}, which properties are well discussed in the literature. The aim of this work was to implement and validate a methodology for simultaneous determination of major and minor constituents in the hydroxyapatite (HA) as well as providing the Ca / P molar ratio. To accomplish these achievements, wavelength dispersive X-ray fluorescence spectroscopy (WDXRF) was applied. This is a non-destructive technique that requires no chemical treatment, enabling fast chemical analysis in a wide variety of samples, with no hazardous waste being generated as a result of the process of determination. A standard reference material from NIST (SRM 1400 – Bone Ash) was used to validate the methodology for the determination of magnesium, phosphorus, potassium, calcium, iron, zinc, strontium and the Ca / P ratio in HA samples by WDXRF. The Z-score test was applied as a statistical tool and showed that the calculated values were of less than 1.8 for all the measured analytes. (author)
Partial pressure (or fugacity) of carbon dioxide, dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the ATLANTIS II in the North Atlantic Ocean from 1989-04-20 to 1989-06-06 (NODC Accession 0113522)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113522 includes chemical, discrete sample, physical and profile data collected from ATLANTIS II in the North Atlantic Ocean from 1989-04-20 to...
In vitro study of RRS HA injectable mesotherapy/biorevitalization product on human skin fibroblasts and its clinical utilization

Directory of Open Access Journals (Sweden)

Deglesne PA

2016-02-01

Full Text Available Pierre-Antoine Deglesne,* Rodrigo Arroyo,* Evgeniya Ranneva, Philippe Deprez Research and Development, SKIN TECH PHARMA GROUP, Castelló d'Empúries, Spain *These authors contributed equally to this work Abstract: Mesotherapy/biorevitalization with hyaluronic acid (HA is a treatment approach currently used for skin rejuvenation. Various products with a wide range of polycomponent formulations are available on the market. Most of these formulations contain noncross-linked HA in combination with a biorevitalization cocktail, formed by various amounts of vitamins, minerals, amino acids, nucleotides, coenzymes, and antioxidants. Although ingredients are very similar among the different products, in vitro and clinical effects may vary substantially. There is a real need for better characterization of these products in terms of their action on human skin or in vitro skin models. In this study, we analyzed the effect of the RRS® (Repairs, Refills, Stimulates HA injectable medical device on human skin fibroblasts in vitro. Skin fibroblast viability and its capacity to induce the production of key extracellular matrix were evaluated in the presence of different concentrations of RRS HA injectable. Viability was evaluated through colorimetric MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay, and key extracellular matrix genes, type I collagen and elastin, were quantified by quantitative polymerase chain reaction. Results demonstrated that RRS HA injectable could promote human skin fibroblast viability (+15% and increase fibroblast gene expression of type I collagen and elastin by 9.7-fold and 14-fold in vitro, respectively. These results demonstrate that mesotherapy/biorevitalization products can, at least in vitro, effectively modulate human skin fibroblasts.Keywords: mesotherapy, medical device, RRS, collagen, elastin, extracellular matrix
Spectral properties of chlorines and electron transfer with their participation in the photosynthetic reaction center of photosystem II

Science.gov (United States)

Shchupak, E. E.; Ivashin, N. V.

2014-02-01

Structural factors that provide localization of excited states and determine the properties of primary donor and acceptor of electron in the reaction center of photosystem II (PSII RC) are studied. The results of calculations using stationary and time-dependent density functional theory indicate an important role of protein environments of chlorophylls PA, PB, BA, and BB and pheophytins HA and HB in the area with a radius of no greater than ≤10 Å in the formation of excitonic states of PSII RC. When the neighboring elements are taken into account, the wavelength of long-wavelength Q y transition of chlorophyll molecules is varied by about 10 nm. The effect is less developed for pheophytin molecules (Δλ ≅ 2 nm). The following elements strongly affect energy of the transition: HisA198 and HisD197 amino-acid residues that serve as ligands of magnesium atoms affect PA and PB, respectively; MetA183 affects PA; MetA172 and MetD198 affect BA; water molecules that are located above the planes of the BA and BB macrocycles form H bonds with carbonyl groups; and phytol chains of PA and PB affect BA, BB, HA, and HB. The analysis of excitonic states, mutual positions of molecular orbitals of electron donors and acceptors, and matrix elements of electron transfer reaction shows that (i) charge separation between BA and HA and PB and BA is possible in the active A branch of cofactors of PSII RC and (ii) electron transfer is blocked at the BB - HB fragment in inactive B branch of PSII RC.
Observed IRIS Profiles of the h and k Doublet of Mg II and Comparison with Profiles from Quiescent Prominence NLTE Models

Czech Academy of Sciences Publication Activity Database

Vial, J. C.; Pelouze, G.; Heinzel, Petr; Kleint, L.; Anzer, U.

2016-01-01

Roč. 291, č. 1 (2016), s. 67-87 ISSN 0038-0938 Institutional support: RVO:67985815 Keywords : Mg II lines * non-LTE diagnostic * Sun prominences Subject RIV: BN - Astronomy, Celestial Mechanics, Astrophysics Impact factor: 2.682, year: 2016
Adsorption of Pb(II), Cu(II), Cd(II), Zn(II), Ni(II), Fe(II), and As(V) on bacterially produced metal sulfides.

Science.gov (United States)

Jong, Tony; Parry, David L

2004-07-01

The adsorption of Pb(II), Cu(II), Cd(II), Zn(II), Ni(II), Fe(II) and As(V) onto bacterially produced metal sulfide (BPMS) material was investigated using a batch equilibrium method. It was found that the sulfide material had adsorptive properties comparable with those of other adsorbents with respect to the specific uptake of a range of metals and, the levels to which dissolved metal concentrations in solution can be reduced. The percentage of adsorption increased with increasing pH and adsorbent dose, but decreased with increasing initial dissolved metal concentration. The pH of the solution was the most important parameter controlling adsorption of Cd(II), Cu(II), Fe(II), Ni(II), Pb(II), Zn(II), and As(V) by BPMS. The adsorption data were successfully modeled using the Langmuir adsorption isotherm. Desorption experiments showed that the reversibility of adsorption was low, suggesting high-affinity adsorption governed by chemisorption. The mechanism of adsorption for the divalent metals was thought to be the formation of strong, inner-sphere complexes involving surface hydroxyl groups. However, the mechanism for the adsorption of As(V) by BPMS appears to be distinct from that of surface hydroxyl exchange. These results have important implications to the management of metal sulfide sludge produced by bacterial sulfate reduction.
Hydroxylamine-induced oxidation of ferrous carbonylated truncated hemoglobins from Mycobacterium tuberculosis and Campylobacter jejuni is limited by carbon monoxide dissociation.

Science.gov (United States)

Ascenzi, Paolo; Ciaccio, Chiara; Gasperi, Tecla; Pesce, Alessandra; Caporaso, Lucia; Coletta, Massimo

2017-08-01

Hydroxylamine (HA) is an oxidant of ferrous globins and its action has been reported to be inhibited by CO, even though this mechanism has not been clarified. Here, kinetics of the HA-mediated oxidation of ferrous carbonylated Mycobacterium tuberculosis truncated hemoglobin N and O (Mt-trHbN(II)-CO and Mt-trHbO(II)-CO, respectively) and Campylobacter jejuni truncated hemoglobin P (Cj-trHbP(II)-CO), at pH 7.2 and 20.0 °C, are reported. Mixing Mt-trHbN(II)-CO, Mt-trHbO(II)-CO, and Cj-trHbP(II)-CO solution with the HA solution brings about absorption spectral changes reflecting the disappearance of the ferrous carbonylated derivatives with the concomitant formation of the ferric species. HA oxidizes irreversibly Mt-trHbN(II)-CO, Mt-trHbO(II)-CO, and Cj-trHbP(II)-CO with the 1:2 stoichiometry. The dissociation of CO turns out to be the rate-limiting step for the oxidation of Mt-trHbN(II)-CO, Mt-trHbO(II)-CO, and Cj-trHbP(II)-CO by HA. Values of the second-order rate constant for HA-mediated oxidation of Mt-trHbN(II)-CO, Mt-trHbO(II)-CO, and Cj-trHbP(II)-CO range between 8.8 × 10 4 and 8.6 × 10 7 M -1 s -1 , reflecting different structural features of the heme distal pocket. This study (1) demonstrates that the inhibitory effect of CO is linked to the dissociation of this ligand, giving a functional basis to previous studies, (2) represents the first comparative investigation of the oxidation of ferrous carbonylated bacterial 2/2 globins belonging to the N, O, and P groups by HA, (3) casts light on the correlation between kinetics of HA-mediated oxidation and carbonylation of globins, and (4) focuses on structural determinants modulating the HA-induced oxidation process.
The lesson learnt during interact - I and INTERACT - II actris measurement campaigns

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Rosoldi Marco

2018-01-01

Full Text Available The INTERACT-II (INTERcomparison of Aerosol and Cloud Tracking campaign, performed at the CNR-IMAA Atmospheric Observatory (760 m a.s.l., 40.60° N, 15.72° E, aims to evaluate the performances of commercial automatic lidars and ceilometers for atmospheric aerosol profiling, through the comparison with Potenza EARLINET (European Aerosol Research Lidar NETwork lidars. The results of the campaign and the overall lesson learnt within INTERACT-I and INTERACT-II ACTRIS campaigns will be presented.
Integrating Seasonal Oscillations into Basel II Behavioural Scoring Models

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Goran Klepac

2007-09-01

Full Text Available The article introduces a new methodology of temporal influence measurement (seasonal oscillations, temporal patterns for behavioural scoring development purposes. The paper shows how significant temporal variables can be recognised and then integrated into the behavioural scoring models in order to improve model performance. Behavioural scoring models are integral parts of the Basel II standard on Internal Ratings-Based Approaches (IRB. The IRB approach much more precisely reflects individual risk bank profile.A solution of the problem of how to analyze and integrate macroeconomic and microeconomic factors represented in time series into behavioural scorecard models will be shown in the paper by using the REF II model.
Caveolin-1 down-regulation is required for Wnt5a-Frizzled 2 signalling in Ha-RasV12 -induced cell transformation.

Science.gov (United States)

Lin, Hsiu-Kuan; Lin, Hsi-Hui; Chiou, Yu-Wei; Wu, Ching-Lung; Chiu, Wen-Tai; Tang, Ming-Jer

2018-05-01

Caveolin-1 (Cav1) is down-regulated during MK4 (MDCK cells harbouring inducible Ha-Ras V12 gene) transformation by Ha-Ras V12 . Cav1 overexpression abrogates the Ha-Ras V12 -driven transformation of MK4 cells; however, the targeted down-regulation of Cav1 is not sufficient to mimic this transformation. Cav1-silenced cells, including MK4/shCav1 cells and MDCK/shCav1 cells, showed an increased cell area and discontinuous junction-related proteins staining. Cellular and mechanical transformations were completed when MDCK/shCav1 cells were treated with medium conditioned by MK4 cells treated with IPTG (MK4+I-CM) but not with medium conditioned by MK4 cells. Nanoparticle tracking analysis showed that Ha-Ras V12 -inducing MK4 cells increased exosome-like microvesicles release compared with their normal counterparts. The cellular and mechanical transformation activities of MK4+I-CM were abolished after heat treatment and exosome depletion and were copied by exosomes derived from MK4+I-CM (MK4+I-EXs). Wnt5a, a downstream product of Ha-Ras V12 , was markedly secreted by MK4+I-CM and MK4+I-EXs. Suppression of Wnt5a expression and secretion using the porcupine inhibitor C59 or Wnt5a siRNA inhibited the Ha-Ras V12 - and MK4+I-CM-induced transformation of MK4 cells and MDCK/shCav1 cells, respectively. Cav1 down-regulation, either by Ha-Ras V12 or targeted shRNA, increased frizzled-2 (Fzd2) protein levels without affecting its mRNA levels, suggesting a novel role of Cav1 in negatively regulating Fzd2 expression. Additionally, silencing Cav1 facilitated the internalization of MK4+I-EXs in MDCK cells. These data suggest that Cav1-dependent repression of Fzd2 and exosome uptake is potentially relevant to its antitransformation activity, which hinders the activation of Ha-Ras V12 -Wnt5a-Stat3 pathway. Altogether, these results suggest that both decreasing Cav1 and increasing exosomal Wnt5a must be implemented during Ha-Ras V12 -driven cell transformation. © 2018 The Authors
Topical stabilized retinol treatment induces the expression of HAS genes and HA production in human skin in vitro and in vivo.

Science.gov (United States)

Li, Wen-Hwa; Wong, Heng-Kuan; Serrano, José; Randhawa, Manpreet; Kaur, Simarna; Southall, Michael D; Parsa, Ramine

2017-05-01

Skin Aging manifests primarily with wrinkles, dyspigmentations, texture changes, and loss of elasticity. During the skin aging process, there is a loss of moisture and elasticity in skin resulting in loss of firmness finally leading to skin sagging. The key molecule involved in skin moisture is hyaluronic acid (HA), which has a significant water-binding capacity. HA levels in skin decline with age resulting in decrease in skin moisture, which may contribute to loss of firmness. Clinical trials have shown that topically applied ROL effectively reduces wrinkles and helps retain youthful appearance. In the current study, ROL was shown to induce HA production and stimulates the gene expression of all three forms of hyaluronic acid synthases (HAS) in normal human epidermal keratinocytes monolayer cultures. Moreover, in human skin equivalent tissues and in human skin explants, topical treatment of tissues with a stabilized-ROL formulation significantly induced the gene expression of HAS mRNA concomitant with an increased HA production. Finally, in a vehicle-controlled human clinical study, histochemical analysis confirmed increased HA accumulation in the epidermis in ROL-treated human skin as compared to vehicle. These results show that ROL increases skin expression of HA, a significant contributing factor responsible for wrinkle formation and skin moisture, which decrease during aging. Taken together with the activity to increase collagen, elastin, and cell proliferation, these studies establish that retinol provides multi-functional activity for photodamaged skin.
A multi-scaled hybrid orthopedic implant: bone ECM-shaped Sr-HA nanofibers on the microporous walls of a macroporous titanium scaffold.

Science.gov (United States)

Han, Yong; Zhou, Jianhong; Zhang, Lan; Xu, Kewei

2011-07-08

We report here, for the first time, a novel multi-scaled hybrid orthopedic implant material consisting of a macroporous Ti scaffold, whose macropores' walls have a microporous titania layer which is fully covered with nanofibers of Sr-doped hydroxyapatite (Sr-HA). The microporous titania layer is formed on and within the Ti scaffold by micro-arc oxidation, which firmly binds to the Ti substrate and contains Ca2+, Sr2+ and PO4(3-) ions. It is then hydrothermally treated to form Sr-HA nanofibers. During the hydrothermal treatment, Sr-HA nanoprisms nucleate from Ca0.5Sr0.5TiO3 pre-formed on the TiO2 and grow in length to nanofibers at the expense of Ca2+, Sr2+ and PO4(3-) ions that migrate from the TiO2. These Sr-HA nanofibers construct a network structure similar to the hierarchical organization of bone extracellular matrix (ECM), and the resulting nanofibrous surface displays a firm adhesion to substrate, superhydrophilicity and apatite-inducing ability. The induced apatite prefers to nucleate on the basal-faceted surfaces of Sr-HA nanofibers. The nanofiber-walled scaffold has a great potential for load-bearing orthotopic use.
Plan for the Startup of HA-21I Furnace Operations at the Plutonium Finishing Plant (PFP)

International Nuclear Information System (INIS)

WILLIS, H.T.

2000-01-01

Achievement of Thermal Stabilization mission elements require the installation and startup of three additional muffle furnaces for the thermal stabilization of plutonium and plutonium bearing materials at the Plutonium Finishing Plant (PFP). The release to operate these additional furnaces will require an Activity Based Startup Review. The conduct of the Activity Based Startup Review (ABSR) was approved by Fluor Daniel Hanford on October 15, 1999. This plan has been developed with the objective of identifying those activities needed to guide the controlled startup of five furnaces from authorization to unrestricted operations by adding the HA-211 furnaces in an orderly and safe manner after the approval to Startup has been given. The Startup Plan provides a phased approach that bridges the activities between the completion of the Activity Based Startup Review authorizing the use of the three additional furnaces and the unrestricted operation of the five thermal stabilization muffle furnaces. The four phases are: (1) the initiation of five furnace operations using three empty (simulated full) boat charges from HA-211 and two full charges from HC-21C; (2) three furnace operations (one full charge from HA-211 and two full charges from HC-21C); (3) four furnace operations (two full charges from HA-211 and two full charges from HC-21C); and (4) integrated five furnace operations and unrestricted operations. Phase 1 of the Plan will be considered as the cold runs. This Plan also provides management oversight and administrative controls that are to be implemented until unrestricted operations are authorized. It also provides a formal review process for ensuring that all preparations needed for full five furnace operations are completed and formally reviewed prior to proceeding to the increased activity levels associated with five furnace operations. Specific objectives include: (1) To ensure that activities are conducted in a safe manner. (2) To provide supplemental

MiR-30e suppresses proliferation of hepatoma cells via targeting prolyl 4-hydroxylase subunit alpha-1 (P4HA1) mRNA

Energy Technology Data Exchange (ETDEWEB)

Feng, Guoxing [State Key Laboratory of Medicinal Chemical Biology, Department of Cancer Research, College of Life Sciences, Nankai University, Tianjin (China); Shi, Hui [State Key Laboratory of Medicinal Chemical Biology, Department of Biochemistry, College of Life Sciences, Nankai University, Tianjin (China); Li, Jiong; Yang, Zhe [State Key Laboratory of Medicinal Chemical Biology, Department of Cancer Research, College of Life Sciences, Nankai University, Tianjin (China); Fang, Runping; Ye, Lihong [State Key Laboratory of Medicinal Chemical Biology, Department of Biochemistry, College of Life Sciences, Nankai University, Tianjin (China); Zhang, Weiying, E-mail: zhwybao@nankai.edu.cn [State Key Laboratory of Medicinal Chemical Biology, Department of Cancer Research, College of Life Sciences, Nankai University, Tianjin (China); Zhang, Xiaodong, E-mail: zhangxd@nankai.edu.cn [State Key Laboratory of Medicinal Chemical Biology, Department of Cancer Research, College of Life Sciences, Nankai University, Tianjin (China)

2016-04-08

Aberrant microRNA expression has been shown to be characteristic of many cancers. It has been reported that the expression levels of miR-30e are decreased in liver cancer tissues. However, the role of miR-30e in hepatocellular carcinoma remains poorly understood. In the present study, we investigated the significance of miR-30e in hepatocarcinogenesis. Bioinformatics analysis reveals a putative target site of miR-30e in the 3′-untranslated region (3′UTR) of prolyl 4-hydroxylase subunit alpha-1 (P4HA1) mRNA. Moreover, luciferase reporter gene assays verified that miR-30e directly targeted 3′UTR of P4HA1 mRNA. Then, we demonstrated that miR-30e was able to reduce the expression of P4HA1 at the levels of mRNA and protein using reverse transcription-polymerase chain reaction and Western blot analysis. Enforced expression of miR-30e suppressed proliferation of HepG2 cells by 5-ethynyl-2-deoxyuridine (EdU) assay and reduced colony formation of these cells by colony formation analysis. Conversely, anti-miR-30e enhanced the proliferation of hepatoma cells in vitro. Interestingly, the ectopic expression of P4HA1 could efficiently rescue the inhibition of cell proliferation mediated by miR-30e in HepG2 cells. Meanwhile, silencing of P4HA1 abolished the anti-miR-30e-induced proliferation of cells. Clinically, quantitative real-time PCR showed that miR-30e was down-regulated in liver tumor tissues relative to their peritumor tissues. The expression levels of miR-30e were negatively correlated to those of P4HA1 mRNA in clinical liver tumor tissues. Thus, we conclude that miR-30e suppresses proliferation of hepatoma cells through targeting P4HA1 mRNA. Our finding provides new insights into the mechanism of hepatocarcinogenesis. - Highlights: • P4HA1 is a novel target gene of miR-30e. • P4HA1 is increased in clinical HCC tissues. • MiR-30e is negatively correlated with P4HA1 in clinical HCC tissues. • MiR-30e suppresses the proliferation of HCC cells through
Investigation of the HA film deposited on the porous Ti6Al4V alloy prepared via additive manufacturing

International Nuclear Information System (INIS)

Surmeneva, M; Chudinova, E; Syrtanov, M; Surmenev, R; Koptioug, A

2015-01-01

This study is focused on the use of radio frequency magnetron sputtering to modify the surface of porous Ti6Al4V alloy fabricated via additive manufacturing technology. The hydroxyapatite (HA) coated porous Ti6Al4V alloy was studied in respect with its chemical and phase composition, surface morphology, water contact angle and hysteresis, and surface free energy. Thin nanocrystalline HA film was deposited while its structure with diamond-shaped cells remained unchanged. Hysteresis and water contact angle measurements revealed an effect of the deposited HA films, namely an increased water contact angle and contact angle hysteresis. The increase of the contact angle of the coating-substrate system compared to the uncoated substrate was attributed to the multiscale structure of the resulted surfaces. (paper)
AFLP and AMP Fingerprints as Markers to Evaluate Genetic Differences between Medicago truncatula Line Jemalong and 2HA, a New Line Produced by in vitro Culture Selection

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R.R. Irwanto

2008-09-01

Full Text Available A new line, Medicago truncatula cv. Jemalong 2HA (herein known as 2HA has been developed via repetitive regeneration and selection of M. truncatula cv. Jemalong. During somatic embryogenesis, 2HA produces 500 times more embryos than its progenitor, Jemalong. It is interesting to study if those two lines are isogenic or has genetic differences. The main objectives of the study was to evaluate the genotypic differences between Jemalong and 2HA also to evaluate the methylation event in 2HA utilized two DNA fingerprinting techniques, i.e AFLP fingerprints (Amplified Length of Polymorphism and AMP (Amplified Methylation Polymorphism. The results showed that AFLP analysis using eight primers combinations could not detect any differences between Jemalong and 2HA. However, using AMP methylation sensitive primers it could detect 15 polymorphisms out of 840 markers. These results lead to a conclusion that Jemalong and 2HA are isogenic lines. 2HA may have higher regeneration capacities due to methylation process which occurs during the production of 2HA through repetitive regeneration cycles.
Distribution of active organic matter in the soil profiles of natural and agricultural ecosystems

Science.gov (United States)

Khodzhaeva, A. K.; Semenov, V. M.

2015-12-01

The amount of active (potentially mineralizable) organic carbon (C0) in the 1-m-deep layer of typical chernozem, dark-gray forest soil, and gray forest soil was estimated for virgin plots and arable land. It was shown that C0 is mainly found in the topsoil (0-20 cm), where its pool reaches 32-60% of the total amount of C0 in the layer of 0-100 cm. The C0 content and its portion in the total organic carbon decrease down the soil profiles. The disturbance of the structure of the pool of active organic carbon—the loss of the moderately mineralizable (0.1 > k 2 > 0.1 day-1) fraction—takes place in the upper horizon of plowed soils. The total pool of C0 in the upper meter of typical chernozem under cropland and under meadow-steppe cenosis comprises 2.8 and 5.2 t/ha, respectively; for the dark gray forest soil under cropland and forest, it reaches 5.5 and 9.8 t/ha, respectively; and for the gray forest soil under cropland and forest, 2.4 and 3.4 t/ha, respectively. The pools of C0 in the typical chernozem. dark gray forest, and gray forest soils are comparable with the values of the annual C-CO2 emission from the soils of these zones.
Identification of miRNAs associated with recurrence of stage II colorectal cancer

DEFF Research Database (Denmark)

Christensen, Lise Lotte; Tobiasen, Heidi; Schepeler, Troels

2011-01-01

Colorectal cancer (CRC) is one of the leading causes of cancer deaths. Twenty-five percent of the patients radically treated for a stage II CRC (no lymph node or distant metastasis) later develop recurrence and dies from the disease. MicroRNAs (miRNAs) are aberrantly expressed or mutated in human...... target prediction and transcript profiling. Initially, miRNA over-expression in HCT116 cells was followed by transcriptional profiling of transfected cells using GeneChip Human Exon 1.0 ST Arrays. Three in silico predicted miRNA targets showing differential mRNA expression upon miRNA up-regulation were...... cancers, and function either as tumour suppressors or oncogenes. Additionally, they also appear to have both diagnostic and prognostic significance. The aim of the present study was to identify miRNAs associated with recurrence of stage II CRC, followed up by an investigation of how these potential...
Temperature profiles from XBT casts from the ATLANTIS II and other platforms as part of the International Decade of Ocean Exploration / International Ocean Studies / First Dynamic Response and Kinematics Experiment in the Drake Passage (IDOE/ISOS/FDRAKE) from 1980-09-17 to 1982-12-10 (NODC Accession 8300003)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts from the ATLANTIS II and other platforms from 17 September 1980 to 10 December 1982. Data were collected by Woods...
Amino Acid Substitutions Improve the Immunogenicity of H7N7HA Protein and Protect Mice against Lethal H7N7 Viral Challenge.

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Subaschandrabose Rajesh Kumar

Full Text Available Avian influenza A H7N7/NL/219/03 virus creates a serious pandemic threat to human health because it can transmit directly from domestic poultry to humans and from human to human. Our previous vaccine study reported that mice when immunized intranasally (i.n with live Bac-HA were protected from lethal H7N7/NL/219/03 challenge, whereas incomplete protection was obtained when administered subcutaneously (s.c due to the fact that H7N7 is a poor inducer of neutralizing antibodies. Interestingly, our recent vaccine studies reported that mice when vaccinated subcutaneously with Bac-HA (H7N9 was protected against both H7N9 (A/Sh2/2013 and H7N7 virus challenge. HA1 region of both H7N7 and H7N9 viruses are differ at 15 amino acid positions. Among those, we selected three amino acid positions (T143, T198 and I211 in HA1 region of H7N7. These amino acids are located within or near the receptor binding site. Following the selection, we substituted the amino acid at these three positions with amino acids found on H7N9HA wild-type. In this study, we evaluate the impact of amino acid substitutions in the H7N7 HA-protein on the immunogenicity. We generated six mutant constructs from wild-type influenza H7N7HA cDNA by site directed mutagenesis, and individually expressed mutant HA protein on the surface of baculovirus (Bac-HAm and compared their protective efficacy of the vaccines with Bac-H7N7HA wild-type (Bac-HA by lethal H7N7 viral challenge in a mouse model. We found that mice immunized subcutaneously with Bac-HAm constructs T143A or T198A-I211V or I211V-T143A serum showed significantly higher hemagglutination inhibition and neutralization titer against H7N7 and H7N9 viruses when compared to Bac-HA vaccinated mice groups. We also observed low level of lung viral titer, negligible weight loss and complete protection against lethal H7N7 viral challenge. Our results indicated that amino acid substitution at position 143 or 211 improve immunogenicity of H7N7HA
In vitro and in vivo performance of bioactive Ti6Al4V/TiC/HA implants fabricated by a rapid microwave sintering technique

International Nuclear Information System (INIS)

Choy, Man Tik; Tang, Chak Yin; Chen, Ling; Wong, Chi Tak; Tsui, Chi Pong

2014-01-01

Failure of the bone–implant interface in a joint prosthesis is a main cause of implant loosening. The introduction of a bioactive substance, hydroxyapatite (HA), to a metallic bone–implant may enhance its fixation on human bone by encouraging direct bone bonding. Ti6Al4V/TiC/HA composites with a reproducible porous structure (porosity of 27% and pore size of 6–89 μm) were successfully fabricated by a rapid microwave sintering technique. This method allows the biocomposites to be fabricated in a short period of time under ambient conditions. Ti6Al4V/TiC/HA composites exhibited a compressive strength of 93 MPa, compressive modulus of 2.9 GPa and microhardness of 556 HV which are close to those of the human cortical bone. The in vitro preosteoblast MC3T3-E1 cells cultured on the Ti6Al4V/TiC/HA composite showed that the composite surface could provide a biocompatible environment for cell adhesion, proliferation and differentiation without any cytotoxic effects. This is among the first attempts to study the in vivo performance of load-bearing Ti6Al4V/TiC and Ti6Al4V/TiC/HA composites in a live rabbit. The results indicated that the Ti6Al4V/TiC/HA composite had a better bone–implant interface compared with the Ti6Al4V/TiC implant. Based on the microstructural features, the mechanical properties, and the in vitro and in vivo test results from this study, the Ti6Al4V/TiC/HA composites have the potential to be employed in load-bearing orthopedic applications. - Highlights: • Ti6Al4V/TiC and Ti6Al4V/TiC/HA composites were fabricated by microwave sintering. • Ti6Al4V/TiC/HA exhibited mechanical properties close to human cortical bone. • Ti6Al4V/TiC/HA could provide a biocompatible environment for bone cell growth. • Ti6Al4V/TiC/HA showed a better bone–implant interface than Ti6Al4V/TiC. • Ti6Al4V/TiC/HA could be used for bone replacement under load-bearing conditions
SAM II aerosol profile measurements, Poker Flat, Alaska; July 16-19, 1979

Science.gov (United States)

Mccormick, M. P.; Chu, W. P.; Mcmaster, L. R.; Grams, G. W.; Herman, B. M.; Pepin, T. J.; Russell, P. B.; Swissler, T. J.

1981-01-01

SAM II satellite measurements during the July 1979 Poker Flat mission, yielded an aerosol extinction coefficient of 0.0004/km at 1.0 micron wavelength, in the region of the stratospheric aerosol mixing ratio peak (12-16 km). The stratospheric aerosol optical depth for these data, calculated from the tropopause through 30 km, is approximately 0.001. These results are consistent with the average 1979 summertime values found throughout the Arctic.
Dissolved inorganic carbon, pH, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, Coulometer for DIC measurement and other instruments from the Ryofu Maru II in the North Pacific Ocean and Philippine Sea from 1997-01-21 to 1997-02-09 (NODC Accession 0112277)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0112277 includes biological, chemical, discrete sample, physical and profile data collected from Ryofu Maru II in the North Pacific Ocean and...
La noción de historia en las encíclicas de Juan Pablo II y su continuidad con el Vaticano II

Directory of Open Access Journals (Sweden)

Sergio Silva G.

2001-01-01

Full Text Available Partiendo de la reafirmación enfática en Fides et Ratio de una frase de Tertio Millennio Adveniente -"en el cristianismo el tiempo tiene una importancia fundamental"-, el artículo recorre las 13 encíclicas de Juan Pablo II, buscando su noción de historia. Se trata, obviamente, de una noción teológica, y se descubre que sus raíces están en el Concilio Vaticano II. La afirmación fundamental de la fe es que, en Jesucristo, Dios ha entrado en la historia y se ha revelado en ella como Amor misericordioso. Para el Papa esto significa que el Dios trascendente, que conduce la historia, se ha hecho en Jesús inmanente a ella. La misión histórica del Hijo es prolongada por la del Espíritu, que no solo anima y conduce a la Iglesia, sino que actúa también fuera de sus límites visibles. En un segundo momento se recogen las afirmaciones acerca de las mediaciones humanas de la presencia de Dios en la historia. Se trata de la Iglesia, que encuentra su modelo en la mediación de María. Respecto de las relaciones de la Iglesia con la historia humana, además de señalar que hay un aporte recíproco, el acento está puesto en tres afirmaciones: la inculturación es la condición de la relación de la Iglesia con la historia, el hombre es el camino de la Iglesia en la historia, y hay una prioridad de la verdad por sobre la libertad y de la dimensión escatológica de la salvación, presente ya en su dimensión temporal, por sobre esta. Finalmente, se recogen y sistematizan las indicaciones respecto a las estructuras creadas involucradas en la mediación histórica de la acción salvífica de Dios. Se trata de la relación del individuo humano con la historia, de los signos de los tiempos (situados al interior de un diagnóstico de la situación actual del mundo y de la mediación hermenéutica (vinculada al papel de la tradición. Como conclusión se reproducen los textos de las Encíclicas en que Juan Pablo II afirma que la tarea prioritaria de su
Comparative histomorphometric analysis between α-Tcp cement and β-Tcp/Ha granules in the bone repair of rat calvaria

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Gisela Grandi

2011-03-01

Full Text Available This study compared the effect of two bioceramics on the process of bone repair: α-tricalcium phosphate (α-TCP cement and β-tricalcium phosphate hydroxyapatite particles (β-TCP/HA. Calvarial defects were created in 50 rats, divided into two groups (α and β/HA. Software was used at 7, 21, 60, 90 and 120 days to assess bone formation. Mean new bone formation rates were as follows: α group, 1.6% at 7 days, 5.24% at 21 days, 24% at 60 days, 30.21% at 90 days and 50.59% at 120 days; β/HA group, 1.94% at 7 days, 2.53% at 21 days, 12.47% at 60 days, 26.84% at 90 days and 38.82% at 120 days; control group, 0.15% at 7 days, 10.12% at 21 days, 15.10% at 60 days, 18.94% at 90 days, 48.50% at 120 days. Both materials are osteoconductive and biocompatible. Perhaps the larger rate of new bone formation observed in the α-TCP group, it also occurs in the β-TCP/HA group within a longer time period.
NBI Calculations for the TJ-II Experimental Discharges; Ajustes de los Perfiles Radiales de Densidad y Temperatura para las Descargas con NBI del TJ-II

Energy Technology Data Exchange (ETDEWEB)

Guasp, J.; Fuentes, C.; Liniers, M.

2005-07-01

The density and electron temperature radial profiles, corresponding to the experimental TJ-II campaigns 2003-2004, with NBI, have been fitted to simple functionals in order to allow a fast approximative evaluation for any given density and injected power... The fits have been calculated, separately, for the four possibilities: ECRH and NBI Phases as well as On and Off Axis ECRH injection. The average difference between the experimental profiles for the individual discharges and the fit predictions are around 8% for the density and 10% for the temperature. The behaviour of the predicted profiles with average line density and injected power has been analysed. The central electron temperature decreases monotonically with increasing density and the ECRH phase On Axis central value is clearly higher than the Off axis one. The radial density profiles narrow with increasing density and the NBI On axis case is clearly wider than de Off one. The electron temperature profile widens slightly with increasing density and the width of the On Axix case is lesser than for the Off case in all phases. There exist Fortran subroutines, available at the three CIEMAT computers, allowing the fast approximative evaluation of all these profiles. (Author) 8 refs.
Increase in the fraction of necrotic, not apoptotic, cells in SiHa xenograft tumours shortly after irradiation

International Nuclear Information System (INIS)

Olive, P.L.; Vikse, C.M.; Vanderbyl, S.

1999-01-01

Background and purpose: Approximately 18% of the cells recovered by rapid mechanical dissociation of SiHa xenograft tumours contain large numbers of DNA strand breaks. The number of damaged cells increases to 30-40% 4-6 h after exposure to 5 or 15 Gy, returning to normal levels by 12 h. This observation is reminiscent of the rate of production of apoptotic cells in other murine and human xenograft tumours. The nature of this damage, rate of development and relation to cell proliferation rate were therefore examined in detail.Materials and methods: SiHa human cervical carcinoma cells were grown as xenograft tumours in SCID mice. Single-cell suspensions were prepared as a function of time after irradiation of the mouse and examined for DNA damage using the alkaline comet assay. Cell cycle progression was measured by flow cytometry evaluation of anti-bromodeoxyuridine-labelled tumour cells.Results: Significant numbers of apoptotic cells could not be detected in irradiated SiHa tumours using an end-labelling assay, electron microscopy, or histological examination of thin sections. Instead, xenograft cells exhibiting extensive DNA damage in the comet assay were predominantly necrotic cells. The increase in the proportion of heavily damaged cells 4-6 h after irradiation could be the result of an interplay between several factors including loss of viable cells and change in production or loss of necrotic cells. Analysis of the progression of BrdUrd-labelled cells confirmed that while 35% of cells from untreated SiHa tumours had divided and entered G 1 phase by 6 h after BrdUrd injection, none of the labelled cells from tumours exposed to 5 or 15 Gy had progressed to G 1 .Conclusions: The increase in the percentage of SiHa tumour cells with extensive DNA damage 4-6 h after irradiation is attributable to necrosis, not apoptosis. Cell cycle progression and cell loss are likely to influence the kinetics of appearance of both apoptotic and necrotic cells in irradiated tumours
Synthesis and spectroscopic studies of biologically active tetraazamacrocyclic complexes of Mn(II, Co(II, Ni(II, Pd(II and Pt(II

Directory of Open Access Journals (Sweden)

Monika Tyagi

2014-01-01

Full Text Available Complexes of Mn(II, Co(II, Ni(II, Pd(II and Pt(II were synthesized with the macrocyclic ligand, i.e., 2,3,9,10-tetraketo-1,4,8,11-tetraazacycoletradecane. The ligand was prepared by the [2 + 2] condensation of diethyloxalate and 1,3-diamino propane and characterized by elemental analysis, mass, IR and 1H NMR spectral studies. All the complexes were characterized by elemental analysis, molar conductance, magnetic susceptibility measurements, IR, electronic and electron paramagnetic resonance spectral studies. The molar conductance measurements of Mn(II, Co(II and Ni(II complexes in DMF correspond to non electrolyte nature, whereas Pd(II and Pt(II complexes are 1:2 electrolyte. On the basis of spectral studies an octahedral geometry has been assigned for Mn(II, Co(II and Ni(II complexes, whereas square planar geometry assigned for Pd(II and Pt(II. In vitro the ligand and its metal complexes were evaluated against plant pathogenic fungi (Fusarium odum, Aspergillus niger and Rhizoctonia bataticola and some compounds found to be more active as commercially available fungicide like Chlorothalonil.
Structural profiling and biological performance of phospholipid-hyaluronan functionalized single-walled carbon nanotubes

DEFF Research Database (Denmark)

Dvash, Ram; Khatchatouriants, Artium; Solmesky, Leonardo J

2013-01-01

In spite of significant insolubility and toxicity, carbon nanotubes (CNTs) erupt into the biomedical research, and create an increasing interest in the field of nanomedicine. Single-walled CNTs (SWCNTs) are highly hydrophobic and have been shown to be toxic while systemically administrated. Thus...... an inflammatory response in macrophages as evidenced by the cytokine profiling and the use of image-based high-content analysis approach in contrast to non-modified CNTs. In addition, systemic administration of CNT-PL-HA into healthy C57BL/6 mice did not alter the total number of leukocytes nor increased liver...
Generation of Oxtr cDNA(HA)-Ires-Cre Mice for Gene Expression in an Oxytocin Receptor Specific Manner.

Science.gov (United States)

Hidema, Shizu; Fukuda, Tomokazu; Hiraoka, Yuichi; Mizukami, Hiroaki; Hayashi, Ryotaro; Otsuka, Ayano; Suzuki, Shingo; Miyazaki, Shinji; Nishimori, Katsuhiko

2016-05-01

The neurohypophysial hormone oxytocin (OXT) and its receptor (OXTR) have critical roles in the regulation of pro-social behaviors, including social recognition, pair bonding, parental behavior, and stress-related responses. Supporting this hypothesis, a portion of patients suffering from autism spectrum disorder have mutations, such as single nucleotide polymorphisms, or epigenetic modifications in their OXTR gene. We previously reported that OXTR-deficient mice exhibit pervasive social deficits, indicating the critical role of OXTR in social behaviors. In the present study, we generated Oxtr cDNA(HA)-Ires-Cre knock-in mice, expressing both OXTR and Cre recombinase under the control of the endogenous Oxtr promoter. Knock-in cassette of Oxtr cDNA(HA)-Ires-Cre consisted of Oxtr cDNA tagged with the hemagglutinin epitope at the 3' end (Oxtr cDNA(HA)), internal ribosomal entry site (Ires), and Cre. Cre was expressed in the uterus, mammary gland, kidney, and brain of Oxtr cDNA(HA)-Ires-Cre knock-in mice. Furthermore, the distribution of Cre in the brain was similar to that observed in Oxtr-Venus fluorescent protein expressing mice (Oxtr-Venus), another animal model previously generated by our group. Social behavior of Oxtr cDNA(HA)-Ires-Cre knock-in mice was similar to that of wild-type animals. We demonstrated that this construct is expressed in OXTR-expressing neurons specifically after an infection with the recombinant adeno-associated virus carrying the flip-excision switch vector. Using this system, we showed the transport of the wheat-germ agglutinin tracing molecule from the OXTR-expressing neurons to the innervated neurons in knock-in mice. This study might contribute to the monosynaptic analysis of neuronal circuits and to the optogenetic analysis of neurons expressing OXTR. © 2015 Wiley Periodicals, Inc.
Curcumin-Zn(II) complex for enhanced solubility and stability: an approach for improved delivery and pharmacodynamic effects.

Science.gov (United States)

Sareen, Rashmi; Jain, Nitin; Dhar, K L

2016-08-01

The aim of present investigation was to prepare Curcumin-Zn(II) complex in a view to enhance solubility, stability and pharmacodynamic effect in experimentally induced ulcerative colitis. Curcumin-Zn(II) complex was prepared by stirring curcumin with anhydrous zinc chloride at a molar ratio of 1:1. The prepared curcumin metallocomplex was characterized by TLC, FTIR, UV spectroscopy and (1)H NMR. In vitro kinetic degradation and solubility of Curcumin and Curcumin-Zn(II) complex was analyzed spectrophotometrically. Pharmacodynamic evaluation of curcumin and its metal complex was assessed in ulcerative colitis in mice. Curcumin showed chelation with zinc ion as confirmed by the TLC, FTIR, UV spectroscopy and (1)H NMR. The results of TLC [Rf value], IR Spectroscopy [shifting of stretching vibrations of υ(C=C) and υ(C=O)], UV spectra [deconvoluted with absorption band at 432-466.4 nm] of Curcumin-Zn(II) complex compared to curcumin confirmed the formation of metallocomplex. (1)HNMR spectra of Curcumin-Zn(II) showed the upfield shift of Ha and Hb. Kinetic stability studies showed metallocomplex with zinc exhibited good stability. In vivo study revealed significant reduction in severity and extent of colonic damage with Curcumin-Zn(II) which were further confirmed by histopathological study. This study recognizes higher solubility and stability of Curcumin-Zn(II) complex and suggested better pharmacodynamic effects.
Estimated SAGE II ozone mixing ratios in early 1993 and comparisons with Stratospheric Photochemistry, Aerosols and Dynamic Expedition measurements

Science.gov (United States)

Yue, G. K.; Veiga, R. E.; Poole, L. R.; Zawodny, J. M.; Proffitt, M. H.

1994-01-01

An empirical time-series model for estimating ozone mixing ratios based on Stratospheric Aerosols and Gas Experiment II (SAGE II) monthly mean ozone data for the period October 1984 through June 1991 has been developed. The modeling results for ozone mixing ratios in the 10- to 30- km region in early months of 1993 are presented. In situ ozone profiles obtained by a dual-beam UV-absorption ozone photometer during the Stratospheric Photochemistry, Aerosols and Dynamics Expedition (SPADE) campaign, May 1-14, 1993, are compared with the model results. With the exception of two profiles at altitudes below 16 km, ozone mixing ratios derived by the model and measured by the ozone photometer are in relatively good agreement within their individual uncertainties. The identified discrepancies in the two profiles are discussed.
Titanium Dioxide Nanoparticle Penetration into the Skin and Effects on HaCaT Cells.

Science.gov (United States)

Crosera, Matteo; Prodi, Andrea; Mauro, Marcella; Pelin, Marco; Florio, Chiara; Bellomo, Francesca; Adami, Gianpiero; Apostoli, Pietro; De Palma, Giuseppe; Bovenzi, Massimo; Campanini, Marco; Filon, Francesca Larese

2015-08-07

Titanium dioxide nanoparticles (TiO2NPs) suspensions (concentration 1.0 g/L) in synthetic sweat solution were applied on Franz cells for 24 h using intact and needle-abraded human skin. Titanium content into skin and receiving phases was determined. Cytotoxicity (MTT, AlamarBlue(®) and propidium iodide, PI, uptake assays) was evaluated on HaCat keratinocytes after 24 h, 48 h, and seven days of exposure. After 24 h of exposure, no titanium was detectable in receiving solutions for both intact and damaged skin. Titanium was found in the epidermal layer after 24 h of exposure (0.47 ± 0.33 μg/cm(2)) while in the dermal layer, the concentration was below the limit of detection. Damaged skin, in its whole, has shown a similar concentration (0.53 ± 0.26 μg/cm(2)). Cytotoxicity studies on HaCaT cells demonstrated that TiO2NPs induced cytotoxic effects only at very high concentrations, reducing cell viability after seven days of exposure with EC50s of 8.8 × 10(-4) M (MTT assay), 3.8 × 10(-5) M (AlamarBlue(®) assay), and 7.6 × 10(-4) M (PI uptake, index of a necrotic cell death). Our study demonstrated that TiO2NPs cannot permeate intact and damaged skin and can be found only in the stratum corneum and epidermis. Moreover, the low cytotoxic effect observed on human HaCaT keratinocytes suggests that these nano-compounds have a potential toxic effect at the skin level only after long-term exposure.

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