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  1. The Effect of Group Discussion on the Quality of Life and HbA1c Levels of Adolescents With Diabetes.

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    Afshar, Mohamad; Memarian, Robabe; Mohammadi, Esa

    2014-08-01

    Diabetes is a metabolic syndrome and the most common endocrine disorder in childhood and adolescence. Diabetes occurs at any age but the highest outbreak is during ten to 15 years of age and 75% of the cases are diagnosed at the age 18. This study aimed to investigate the effect of a group discussion on the quality of life (QOL) and glycosylated hemoglobin A (HbA1c) levels of adolescents with diabetes. This quasi-experimental study was performed on 56 adolescents with diabetes who were referred to Golabchi Diabetes Center in Kashan, Iran. After obtaining written informed consent from the patients, blood sample was drawn for measuring sugar and HbA1c levels. The participants completed the questionnaire regarding the QOL. Patients were randomly allocated to four groups. All the groups attended similar group discussion sessions, which were conducted according to the guidance of diabetic specialists. The groups' members followed the discussed instructions for four months. Then, another questionnaire was completed and blood sugar and HbA1c levels were measured again. The results were compared by paired-samples t-test and Wilcoxon signed-rank test. After the group discussion sessions, in 56% of the patients the HbA1c levels (8.45 ± 1.35 and 6.98 ± 0.89 before and after intervention, respectively) and QOL were improved significantly. The mean age of these patients was 14.75 ± 1.80 years and the mean of daily insulin injection was 35.70 ± 13.42 units. Sharing experiences trough group discussions and receiving instructive feedbacks can improve the QOL and metabolic status of adolescents with diabetes.

  2. A Comparison of hs-CRP Levels in New Diabetes Groups Diagnosed Based on FPG, 2-hPG, or HbA1c Criteria.

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    Tutuncu, Yildiz; Satman, Ilhan; Celik, Selda; Dinccag, Nevin; Karsidag, Kubilay; Telci, Aysegul; Genc, Sema; Issever, Halim; Tuomilehto, Jaakko; Omer, Beyhan

    2016-01-01

    Fasting plasma glucose (FPG) and hemoglobin A1c (HbA1c) have been used to diagnose new-onset diabetes mellitus (DM) in order to simplify the diagnostic tests compared with the 2-hour oral glucose tolerance test (OGTT; 2-hPG). We aimed to identify optimal cut-off points of high sensitive C-reactive protein (hs-CRP) in new-onset DM people based on FPG, 2-hPG, or HbA1c methods. Data derived from recent population-based survey in Turkey (TURDEP-II). The study included 26,499 adult people (63% women, response rate 85%). The mean serum concentration of hs-CRP in women was higher than in men (p hs-CRP level than FPG based and 2-hPG based DM cases. In HbA1c, 2-hPG, and FPG based new-onset DM people, cut-off levels of hs-CRP in women were 2.9, 2.1, and 2.5 mg/L [27.5, 19.7, and 23.5 nmol/L] and corresponding values in men were 2.0, 1.8, and 1.8 mg/L (19.0, 16.9, and 16.9 nmol/L), respectively (sensitivity 60-65% and specificity 54-64%). Our results revealed that hs-CRP may not further strengthen the diagnosis of new-onset DM. Nevertheless, the highest hs-CRP level observed in new-onset DM people diagnosed with HbA1c criterion supports the general assumption that this method might recognize people in more advanced diabetic stage compared with other diagnostic methods.

  3. Drugs affecting HbA1c levels

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    Ranjit Unnikrishnan

    2012-01-01

    Full Text Available Glycated hemoglobin (HbA1c is an important indicator of glycemic control in diabetes mellitus, based on which important diagnostic and therapeutic decisions are routinely made. However, there are several situations in which the level of HbA1c may not faithfully reflect the glycemic control in a given patient. Important among these is the use of certain non-diabetic medications, which can affect the HbA1c levels in different ways. This review focuses on the non-diabetic medications which can inappropriately raise or lower the HbA1c levels, and the postulated mechanisms for the same.

  4. The Effect of Group Counseling on Physiological Aspect of Self-care and HbA1C Level of Patients with Diabetes Type II

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    Seyedreza Mazlom; Mahbobeh Firooz; Farzane Hasanzade; Seyedali Kimiaee; Aliakbar Raoufsaeb

    2015-01-01

    Background: The most important underlying cause of death in diabetic patients is poor self-care. The effect of education on self-care promotion has been widely investigated; however, the advisory role and impact of the treatment team have been scarcely investigated.  Aim: Determining the effect of group counseling on the psychological aspect of self-care and level of glycosylated hemoglobin in the patients with diabetes type II. Methods: In a randomized clinical trial, 73 patients with type I...

  5. Association between blood group and susceptibility to malaria and its effects on platelets, TLC, and Hb.

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    Burhan, Hira; Hasan, Askari Syed; Mansur-Ul-Haque, Syed; Zaidi, Ghazanfar; Shaikh, Taha; Zia, Aisha

    2016-10-31

    According to the World Health Organization, the estimated number of malaria cases in Pakistan is about 1.5 million. Hematological variables like platelets, total leukocyte count (TLC), and hemoglobin (Hb) need to be evaluated to diagnose malaria in suspects. This study aimed to investigate the association between blood group and susceptibility to malaria and effects on platelets, TLC, and Hb. This was a case-control study with a sample size of 446, of which 224 were malarial cases and 222 were controls. A designated questionnaire was developed to know age, gender, malarial strain, Hb, TLC, platelets, and blood group. Of 224 malarial cases, 213 were P. vivax, and 11 were P. falciparum. There were 58 patients with blood group A, 72 with group B, 69 were O and 23 were AB. There was no significant difference in the blood group of controls compared to malarial patients (p > 0.05). Mean Hb level was 11.5mg/dL in malaria patients and 12.5mg/dL in controls. There was significant difference (pTLC was not significant between malarial and control groups (p = 0.072). Males were two times susceptible to malaria. There was no significant association between the type of blood group and susceptibility to malaria or developing anemia or thrombocytopenia.

  6. Periodontal treatment and HbA1c levels in subjects with diabetes mellitus.

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    Altamash, M; Klinge, B; Engström, P-E

    2016-01-01

    It has earlier been reported that individuals with poorly controlled diabetes have severe periodontal disease (PD) compared to well-controlled diabetes. This longitudinal interventional study compared periodontal treatment outcomes with HbA1c level changes in four groups of diabetic and non-diabetic patients with or without PD, respectively. HbA1c, bleeding on probing (BOP), plaque index and periodontal pocket depth (PPD) 4 Diabetics with PD and without PD showed reductions in HbA1c levels with a mean value of 0·3% after 3 months and mean values of 1% and 0·8%, respectively, after 6 months. Diabetics with PD showed higher levels of BOP versus non-diabetics without PD (P diabetics without PD (P diabetics with PD showed higher number of PPD 4 diabetics without PD (P diabetics with PD (P Diabetics without PD showed higher levels of PPD 4 diabetics without PD (P periodontal treatment in all groups improved periodontal inflammatory conditions with a decrease in HbA1c levels in a period of three and 6 months. No change was seen in the number of pockets PPD 4 diabetic subjects with PD after non-surgical and surgical treatment. © 2015 John Wiley & Sons Ltd.

  7. [Doping in endurance sports. Survey of individual [Hb] levels can expose doping].

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    Ekblom, B; Holmberg, H C; Eriksson, K

    2001-11-28

    Doping through increasing [Hb] increases physical performance in sport. Therefore, no cross-country skiers with [Hb] values above 160 and 175 g/l for women and men, respectively, may start in competitions. Even plasma expanders have been used, possibly for lowering a high [Hb] but this procedure may not increase physical performance. There are methods available for detecting the use of erythropoietin but not reinfusion of erythrocytes to increase [Hb]. To make it more difficult to increase [Hb] by different unethical methods we suggest that the [Hb] in endurance athletes is determined both during the training and the competition season to establish individual [Hb] mean values and range. Since endurance training at altitude does not increase [Hb] after return to sea level, an occasional increased [Hb] is suspicious. In such a case complementary doping tests may be used.

  8. Trajectories of HbA1c levels in children and youth with type 1 diabetes.

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    Orit Pinhas-Hamiel

    Full Text Available To illustrate the distribution of Hemoglobin A1c (HbA1c levels according to age and gender among children, adolescents and youth with type 1 diabetes (T1DM.Consecutive HbA1c measurements of 349 patients, aged 2 to 30 years with T1DM were obtained from 1995 through 2010. Measurement from patients diagnosed with celiac disease (n = 20, eating disorders (n = 41 and hemoglobinopathy (n = 1 were excluded. The study sample comprised 4815 measurements of HbA1c from 287 patients. Regression percentiles of HbA1c were calculated as a function of age and gender by the quantile regression method using the SAS procedure QUANTREG.Crude percentiles of HbA1c as a function of age and gender, and the modeled curves produced using quantile regression showed good concordance. The curves show a decline in HbA1c levels from age 2 to 4 years at each percentile. Thereafter, there is a gradual increase during the prepubertal years with a peak at ages 12 to 14 years. HbA1c levels subsequently decline to the lowest values in the third decade. Curves of females and males followed closely, with females having HbA1c levels about 0.1% (1.1 mmol/mol higher in the 25th 50th and 75th percentiles.We constructed age-specific distribution curves for HbA1c levels for patients with T1DM. These percentiles may be used to demonstrate the individual patient's measurements longitudinally compared with age-matched patients.

  9. HbA1c Levels among Primary Healthcare Patients with Type 2 Diabetes Mellitus in Oman

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    Jawad A Al-Lawati

    2012-11-01

    Full Text Available Objectives: To investigate whether younger patients with type 2 diabetes mellitus have higher glycated hemoglobin A1c (HbA1c levels compared to older patients, and to determine the factors associated with higher HbA1c levels.Methods: Data from 1,266 patients from all over Oman were used to obtain the mean HbA1c level, odds ratios (OR, and 95% confidence intervals (CI from multiple logistic regression models with age groups, sex, duration of diabetes, diabetes treatment, body mass index, estimated glomerular filtration rate (eGFR, tobacco use, and healthcare index as predictors of good (HbA1c <7% vs.poor (≥7% glycemic control.Results: Mean HbA1c levels were 8.9, 8.3, and 7.8 in the age groups 20-39, 40-59 and 60+ years, respectively. After controlling for all other covariates, the OR of good glycemic control increased with age, 40-59 years old (OR=1.7; 95% CI 1.1 to 2.6 and 60+ year (OR=2.5; 95% CI 1.6 to 4.0, female gender (OR=1.5; 95% CI 1.2 to 2.0 and in patients with eGFR ≥60 mL/min/1.73 m2 (OR=1.9; 95% CI 1.1 to 3.3. Longer duration of diabetes (≥5years and treatment with oral agents or insulin were inversely related to good glycemic control.Conclusion: Younger Omani adults exhibit worse glycemic levels compared to older adults posing a formidable challenge to diabetes care teams.

  10. Evaluation of the relationship between corneal biomechanic and HbA1C levels in type 2 diabetes patients

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    Yazgan S

    2014-08-01

    Full Text Available Serpil Yazgan,1 Ugur Celik,2 Havva Kaldirim,3 Orhan Ayar,1 Ahmet Elbay,4 Veysel Aykut,2 Burcu Celik,5 Mehmet Taş6 1Department of Ophthalmology, Zonguldak Karaelmas University, Zonguldak, Turkey; 2Department of Ophthalmology, Gaziosmanpasa Taksim Training and Research Hospital, Istanbul, Turkey; 3Department of Ophthalmology, Bagcilar Training and Research Hospital, Istanbul, Turkey; 4Department of Ophthalmology, Pendik Government Hospital, Istanbul, Turkey; 5Department of Ophthalmology, Beyoglu Eye Training and Research Hospital, Istanbul, Turkey; 6Department of Ophthalmology, Malatya State Hospital, Malatya, Turkey Purpose: To evaluate the corneal biomechanical properties due to the glycosylated hemoglobin (HbA1C levels using the ocular response analyzer (ORA in the patients with type 2 diabetes mellitus (DM. Methods: ORA values were obtained from 156 eyes of subjects with type 2 DM and 74 eyes of healthy control subjects with similar age and sex. Subjects were divided into three groups: Group 1, healthy control subjects; Group 2, diabetes patients with HbA1C ≥7%; and Group 3, diabetes patients with HbA1C <7%. Corneal biomechanical parameters: corneal hysteresis (CH, corneal resistance factor (CRF, Goldmann-correlated pressure (IOPg, and corneal-compensated intraocular pressure (IOPcc measurements were obtained using ORA. Ultrasound pachymetry was used for measurement of central corneal thickness (CCT. Results: CH and CRF were significantly different in each of the three groups (P-values for CH respectively; Groups 1 and 2=0.008, Groups 1 and 3, and Groups 2 and 3, <0.001, and for CRF respectively; =0.002, <0.001, <0.001. CCT was significantly different between Groups 1 and 3 and Groups 2 and 3 (P<0.001 but was insignificant between Groups 1 and 2 (P=0.965. IOPcc was not different between Groups 1 and 2 (P=0.524, and Groups 2 and 3 (P=0.115, but was significantly different between Groups 1 and 3 (P=0.003. IOPg was statistically different

  11. Predictors of HbA1c levels in patients initiating metformin

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    Martono, Doti P.; Hak, Eelko; Heerspink, Hiddo Lambers; Wilffert, Bob; Denig, Petra

    2016-01-01

    Objective: The aim was to assess demographic and clinical factors as predictors of short (6 months) and long term (18 months) HbA1c levels in diabetes patients initiating metformin treatment. Research design and methods: We conducted a cohort study including type 2 diabetes patients who received the

  12. Effect of Education of Self-care Behaviors on HbA1C level in Diabetic Patients

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    Ahmadi Zakieh

    2016-06-01

    Full Text Available Background and Objective: The successful glycemic control in diabetic patients is very important. Since the poor self-care is reported as the most important causes of mortality and complications, therefore these patients require self-care knowledge. This study aimed to determine the effect of education of self-care behaviors on HbA1C level in diabetic patients. Materials and Method: In this clinical trial, 80 diabetic patients referred to the diabetes clinic in Bandar Abbas were selected through random sampling and then were randomly allocated into two groups of intervention and control based on stratified random sampling. Designed educational program was implemented in 9 sessions (60 minutes, once a week for 12 weeks for patients in the intervention group. Patients in the control group were received usual care of clinic. Data were collected through using demographic information form and HbA1c test. Data were collected at the beginning of the study and 12 weeks after the intervention. Data were analyzed by SPSS software (version 18 using independent T- test, paired T-test, Mann-Whitney U and chi-square. Results: Before the intervention, the HbA1c average in the intervention and control groups was 8.18 ± 1.66 and 8.41 ± 2.10, respectively and after the intervention these values were changed to 7.78 ± 1.48 and 8.82 ± 2.11 in the intervention and control groups respectively (p = 0.01. Conclusion: Implementation of educational program of self-care behaviors was effective in reducing HbA1C and it can be used as an appropriate educational method by nurses and other health care team in controlling of diabetes in patients.

  13. Effect of iron deficiency anemia and iron supplementation on HbA1c levels - Implications for diagnosis of prediabetes and diabetes mellitus in Asian Indians.

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    Madhu, S V; Raj, Abhishek; Gupta, Stuti; Giri, S; Rusia, Usha

    2017-05-01

    We investigated the effect of iron deficiency anemia (IDA) on levels of glycated hemoglobin (HbA1c) and to compare its levels before and after iron supplementations. Age and sex matched subjects were enrolled and clustered in 2 groups: IDA (n=62) and healthy controls (HC; n=60). HbA1c levels were estimated by HPLC. Hemogram were estimated by hematology analyser. Serum ferritin (ELISA) and other parameters of iron profile were measured by standard guidelines of ICSH. HbA1c values and iron studies were repeated after 3months of iron supplementation to determine the effect of iron therapy on HbA1c levels. Significantly higher HbA1c levels were observed in IDA subjects compared to HC (5.51±0.696 v/s 4.85±0.461%, p<0.001). A significant negative correlation was observed between HbA1c and hemoglobin, hematocrit, RBC count, MCH, MCHC and serum ferritin in IDA subjects (r=-0.632, -0.652, -0.384, -0.236, -0.192 and -0.441). Significant decline was noticed in HbA1c levels in IDA subjects after iron supplementation (5.51±0.696 before treatment v/s 5.044±0.603 post-treatment; p<0.001). Post treatment, 70% subjects (14/20) with HbA1c in pre-diabetes range normalised to normal glucose tolerance (NGT) range and out of 6 patients with pre-treatment HbA1c in diabetes range, 5 reverted to pre-diabetes range while 1 of them reverted to the NGT range. Caution must be exercised in interpreting the results of HbA1c in patients of IDA and iron deficiency must be corrected before diagnosing diabetes and pre-diabetes solely on the basis of HbA1c criteria. Copyright © 2016. Published by Elsevier B.V.

  14. Beta-globin gene cluster haplotypes and HbF levels are not the only modulators of sickle cell disease in Lebanon.

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    Inati, A; Taher, A; Bou Alawi, W; Koussa, S; Kaspar, H; Shbaklo, H; Zalloua, P A

    2003-02-01

    Sickle cell disease (SCD) is an inherited autosomal recessive disorder of the beta-globin chain. Despite the fact that all subjects with SCD have the same single base pair mutation, the severity of the clinical and hematological manifestations is extremely variable. This study examined for the first time in Lebanon the correlation between the clinical manifestation of SCD and the beta-globin gene haplotypes. The haplotypes of 50 patients diagnosed with SCD were determined using polymerase chain reaction amplification of fragments containing nine polymorphic restriction sites around and within the epsilon-Ggamma-Agamma-psibeta-delta-beta-globin gene complex. Most reported haplotypes were found in our population with the Benin haplotype as the most prevalent one. When the patients were divided according to their HbF levels into three groups (Group A: HbF 15%), surprisingly, the highest levels of HbF were associated with the most severe clinical cases. Our findings suggest that fetal hemoglobin levels are important but not the only parameters that affect the severity of the disease. In addition, the high levels of HbF in patients with CAR haplotypes did not seem to ameliorate the severity of symptoms, suggesting that genetic factors other than haplotypes are the major determinants of increased HbF levels in Lebanon.

  15. Meta-analysis of telemonitoring to improve HbA1c levels: promise for stroke survivors.

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    Lieber, Bryan A; Taylor, Blake; Appelboom, Geoff; Prasad, Kiran; Bruce, Sam; Yang, Annie; Bruce, Eliza; Christophe, Brandon; Connolly, E Sander

    2015-05-01

    Monitoring glycemic control is useful not only in the primary prevention of stroke in diabetics, but also in the rehabilitation from and secondary prevention of stroke. In an often functionally and neurocognitively impaired population, however, poor compliance with treatment regimens is a major problem. Wireless, telemonitoring glucometers - often integrated into the patient's healthcare system - offer a solution to the compliance issue. We sought to evaluate the effectiveness of telemonitoring technologies in improving long-term glycemic control. A search on www.clinicaltrials.gov, using keywords such as "telemonitoring" and "self-care device" was performed, and five trials were identified that compared hemoglobin A1c (HbA1c) levels of a group receiving standard care (controls) to a group receiving a telemonitoring intervention. Four of the five studies showed a greater reduction in HbA1c in the intervention group compared to controls at 6 months, although only one was statistically significant. There was considerable heterogeneity between studies (I(2)=69.5%, p=0.02), and the random effects model estimated the aggregate effect size for mean difference in reduction of HbA1c levels to be 0.08% (95% confidence interval -0.12% to 0.28%), which was not statistically significant (p=0.42). The varying results may be due to specific factors in the trials that contributed to their large heterogeneity, and further trials are needed to support the role of telemonitoring in improving diabetes management in this population. Nonetheless, in the future telemonitoring may substantially help patients at risk of ischemic stroke and those who require close glucose monitoring. Copyright © 2014 Elsevier Ltd. All rights reserved.

  16. HbA1c Levels Are Associated with Chronic Kidney Disease in a Non-Diabetic Adult Population: A Nationwide Survey (KNHANES 2011-2013.

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    Seok Hui Kang

    Full Text Available Many studies have reported an association between glycated hemoglobin A1c (HbA1c and metabolic syndrome (MetS in non-diabetes patients. Each component of MetS is in fact related to chronic kidney disease (CKD incidence and progression. Therefore, HbA1c in non-diabetic mellitus (DM may be intrinsically associated with the prevalence of CKD. The hypothesis of the present study was that high HbA1c in non-DM patients is associated with CKD.The total number of participants in this study was 24,594. The participants were divided into three groups according to their HbA1c levels: a Low group (6.0% or >42 mmol/mol. The estimated glomerular filtration rate (eGFR was calculated using the Chronic Kidney Disease Epidemiology Collaboration equation.The number of participants allocated to the Low, Middle, and High groups was 8,651, 4,634, and 1,387, respectively. Linear regression analyses were performed to evaluate the association between variables. Standardized β ± standard error was 0.25 ± 0.22 for waist circumference, 0.44 ± 0.20 for fasting glucose, -0.14 ± 0.30 for high-density lipoprotein cholesterol levels, 0.15 ± 2.31 for triglyceride levels, 0.21 ± 0.00 for systolic blood pressure, 0.10 ± 0.00 for diastolic blood pressure, and -0.22 ± 0.42 for eGFR (P < 0.001 for all variables. eGFR in non-diabetes participants was inversely associated with the HbA1c level, where eGFR decreased as HbA1c levels increased. Standardized βs were -0.04 ± 0.42 in multivariable analysis (P < 0.001. The proportion of participants with only MetS, only CKD, or both MetS and CKD was higher in the High group than in the Low and Middle groups.High HbA1c in non-DM patients may be associated with CKD. Renal function in patients with high HbA1c levels may need to be monitored.

  17. 社区2型糖尿病患者HbA1c水平与营养状况的关系研究%Association of HbA1c level with nutritional status in community-based patients with type 2 diabetes

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    徐丹凤; 姜菁静; 孙建琴; 陈艳秋; 陈敏; 谢华; 唐倩如; 孙玮; 陈爱芳; 孙唯佳; 林轶凡

    2010-01-01

    Objective To explore the possible correlation between HbA1c level and nutritional status in community.based patients with type 2 diabetes.Methods A totaI of 219 type 2 diabetes patients were assigned into 2 groups:one with HbAIc≥6.5%者的营养状况差异明显,饮食对HbA1c的影响较大,因此应对2型糖尿病患者进行合理的饮食治疗,尽早实现糖尿病控制达标.

  18. Elevated HbA1c Levels Are Associated with the Blunted Autonomic Response Assessed by Heart Rate Variability during Blood Volume Reduction.

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    Kamakura, Miho; Maruyama, Ryoko

    2016-10-01

    A high glycemic status increases the risk for autonomic dysfunction and cardiovascular failure. The aim of this study was to investigate time-dependent changes in the autonomic response and cardiovascular dynamics and the association between the level of hemoglobin A1c (HbA1c) and autonomic response during blood volume reduction. The study population consisted of 26 preoperative participants who were scheduled for autologous blood donation (200-400 mL of whole blood) for intraoperative or postoperative use. These participants without circulatory, respiratory, or brain disease and diabetes mellitus were grouped according to their HbA1c levels: blood pressure (BP) and analyzed heart rate variability (HRV) to quantify cardiac autonomic regulation throughout blood donation. During blood volume reduction, which was about 10% of the circulating blood volume, the BP and heart rate varied within normal ranges in both groups. The high-frequency (HF) component, an index of parasympathetic nerve activity, and the ratio of low-frequency (LF) to HF components (LF/HF), an index of sympathetic nerve activity, significantly decreased and increased with the progression of blood volume reduction, respectively, in the HbA1c blood volume reduction only in the HbA1c blood volume reduction.

  19. Survey of Air Pollution Effect on Variation of Glycosylated Hemoglobin A1C (HbA1C level in Diabetic Patients in Tehran

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    Fatemeh Mousavi

    2013-05-01

    Materials and Methods: During November-January 2010-11, Tehran, capital of Iran, was exposed with high levels of air pollution. A retrospective cohort study was carried out on 330 patients diagnosed with diabetes mellitus for at least 12 months referring to 3 endocrinal care clinics. A questionnaire in two demographic and diabetic related sections was prepared. The patients' HbA1C level recorded on November-January 2009-10 was compared with November-January 2010-11. Descriptive analysis and paired t-test were carried out using SPSS 18 software. Results: The patients investigated were divided into two groups. The first group was composed of 108 patients (53.7% female and 46.3% male with diabetes mellitus type I (Insulin Dependent, age mean of 17.22, and SD of 11.57. The second group was composed of 222 patients (58.6% female and 41.4% male with diabetes mellitus type II (Noninsulin Dependent, age mean of 53.91, and SD of 12.12. The change of HbAIC level in both groups wa not statistically significant; in first group, HbA1C level increased from 7.71 to 7.75 mg / 100 ml (P =0.828 and in second group, it increased from 7.06 to 7.08 mg / 100 ml (P = 0.798. Conclusion: According to the results obtained, it can be concluded that relation of air pollution and HbA1C mean variation in diabetic patients was insignificant.

  20. Serum testosterone levels of HbSS (sickle cell disease male subjects in Lagos, Nigeria

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    Adediran Adewumi

    2011-08-01

    Full Text Available Abstract Background Infertility is a major problem in sickle cell disease patients, especially in males. In addition to low serum testosterone, other abnormalities involving the accessory sex organs, such as the seminal vesicles and the prostate gland, as well as marked decrease in ejaculate volume may be observed in male HbSS patients. Hence, the need to study the role of sex hormones as a cause of infertility in male HbSS patients. Methods An unmatched case-control study was performed using seventy-five consenting subjects from Lagos University Teaching Hospital. These included 47 patients with haemoglobin phenotype SS from the Sickle cell clinic and 28 volunteered medical students and members of staff with haemoglobin phenotype AA. Demographic data were obtained using a self-administered questionnaire. A total of 5 mls of blood was collected from each subject between 9.00 am & 11.am, and assayed for serum testosterone concentration. Results The concentrations of serum testosterone in HbSS patients ranged from 0.2 to 4.3 ng/ml with a mean of 1.28 ± 0.72 ng/ml whilst the values in HbAA controls ranged from 1.2 to 6.9 ng/ml with a mean of 2.63 ± 1.04 ng/ml. Seven (25.0% of the 28 controls had serum testosterone concentration lower than the quoted reference (normal range whereas 44 (93.6% of the 47 HbSS subjects had serum testosterone concentration lower than the reference range. Conclusion Overall, subjects with HbSS have significantly lower mean serum testosterone than HbAA controls.

  1. Comparison of Serum Zinc Level in Patients with Diabetes Type 1 and 2 and Its' Relation to HbA1c

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    Fatemeh Dorreh

    2014-01-01

    Full Text Available Background: Diabetes Mellitus (DM is a major health care problem. The relationship between DM and zinc has frequently been reported in various research. The present study aims to investigate serum zinc level in patients with type 1 (IDDM and type 2 (NIDDM. Association between glyaceted hemoglobin and level of zinc is also evaluted. Materials and Methods: This cross-sectional study was conducted on 60 subjects with DM (Type l: N=30; Type 2: N=30 who met inclusion criteria of the study. Patients’ serum zinc level and HbA1c were measured. Data were analyzed using t-test and Mann-Withney U test. Results: Seventy five percent of the subjects were female. The average age of the IDDM was 15.36±5.28 years and that of NIDDM was 48.70±11.45 years. The average HbA1c of subjects was 8.06±1.64%. The average serum level of zinc in IDDM group was 95.82±14.51 μg/dl and that of NIDDM was 97.47±32.36 μg/dl, no significant difference was found between the two groups. Serum zinc difficiency was detected in 20% of the patients with NIDDM and 16.6% of the patients with IDDM. However, no significant correlation between HbA1c and serum level of zinc was detected in this study. Conclusion: Zinc deficiency was detected among a significant percentage of IDDM and NIDDM patients, but no significant correlation between serum zinc level and HbA1c was detected.

  2. Molecular Analysis of HS-111 and 3`HS1 Variations in β-Thalassemia Intermedia Patients with High Levels of HbF

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    Mohammad Hamid

    2010-01-01

    Full Text Available Objective: To study the possible association between high levels of fetal haemoglobin(HbF in β-thalassemia intermedia patients and HS-111 and 3`HS1 sequence variations.Materials and Methods: In this study, the 3' HS-1 and HS-111 regions of 30 ß-thalassaemiaintermedia patients (ß°/ß° with high levels of HbF, 21 ß-thalassemia major patientsand 40 normal Iranian individuals were analyzed by single-strand conformation polymorphism(SSCP and polymerase chain reaction (PCR sequencing.Results: Two nucleotide variations in 3' HS111 (-21A>G and 3`HS1 (179C>T wereidentified. The most frequent sequence variation was 3' HS111 (-21A in the intermediapatients and 3`HS111 (-21G in the major thalassemia patients. In contrast to the 3`HS1marker, both 3'HS111 A and G variants showed a correlation with each studied group.Conclusion: The HS111 marker in conjunction with other parameters could be used asappropriate genetic markers to discriminate β-thalassemia intermedia patients (β°/β° withhigh levels of HbF from β-thalassemia major patients.

  3. Relationship between level of HbA1C and breast cancer

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    Fariba Jousheghany

    2016-12-01

    Conclusion: While there was a statistically significant association between HbA1C and obesity, there were no significant associations found with this analysis. However, there are clinically meaningful relationships based on observed trends. Future directions for research may involve exploring a larger sample of patients and the role of therapeutic regimens on blood sugar control and BMI of breast cancer patients and influence on cancer prognosis.

  4. Relationship between the Depression and HbA1 c Level in Elderly with Type 2 Diabetes Mellitus%Ⅱ型糖尿病老年人抑郁与HbA1 c水平关系的研究

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    赵春善; 顾妍春

    2014-01-01

    Objective The aim of this study was to investigate the current situation of depression and HbA1 c level in type 2 diabetes mellitus elderly with depression;to discuss the relationship between the depression and the HbA1clevel in elderly with type 2 diabetes mellitus. Methods 316 elderly with type 2 diabetes mellitus were randomly selected with convenience sampling method for the questionnaire of depression. Results There were 47. 5% type 2 diabetes mellitus elderly with high level of HbA1C,and 25. 6% of them with depression;there were significant differences in FPG,2h-PG and HbA1c between depressive and non-depressive type 2 diabetes mellitus elderly(P<0. 01);the multi-factor logistic regression analysis revealed that ethnic,complication and depression should be important factors to affect the HbA1c level. Conclusions Depressive symptoms in elderly with type 2 diabetes mellitus are ubiquitous, and the ability of depressive patients to control the blood sugar level is poor. The clinical doctors should pay more attention to the psychotherapy in them,to help them to get over the unhealthy psychology and gain the confidence as soon as possible, which may be beneficial to decrease HbA1c level,and then the effective control of blood sugar level.%目的:了解Ⅱ型糖尿病老年人抑郁现状及HbA1c水平,探讨Ⅱ型糖尿病老年人抑郁与HbA1c水平的关系.方法采用便利抽样法对Ⅱ型糖尿病老年人共316名进行抑郁问卷调查,并对其进行相关生化检查.结果47.5%的Ⅱ型糖尿病老年人糖化血红蛋白(HbA1c)呈高值,且抑郁检出率为25.6%;抑郁组与非抑郁组Ⅱ型糖尿病老人FPG,2h-PG及HbA1c等生化指标存在差异(P<0.01);多因素Logistic回归分析结果表明:民族、并发症、抑郁是影响HbA1c水平的重要因素.结论Ⅱ型糖尿病老年人抑郁症状普遍存在,抑郁患者血糖控制能力较差,临床医生应注重糖尿病老年人的心理治疗,帮助患者及早摆脱

  5. Heterogeneous behavior of lipids according to HbA1c levels undermines the plausibility of metabolic syndrome in type 1 diabetes: data from a nationwide multicenter survey

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    Giuffrida Fernando MA

    2012-12-01

    Full Text Available Abstract Background Cardiovascular risk factors (CVRF may cluster in type 1 diabetes, analogously to the metabolic syndrome described in type 2 diabetes. The threshold of HbA1c above which lipid variables start changing behavior is unclear. This study aims to 1 assess the behavior of dyslipidemia according to HbA1c values; 2 detect a threshold of HbA1c beyond which lipids start to change and 3 compare the clustering of lipids and other non-lipid CVRF among strata of HbA1c individuals with type 1 diabetes. Methods Effects of HbA1c quintiles (1st: ≤7.4%; 2nd: 7.5-8.5%; 3rd: 8.6-9.6%; 4th: 9.7-11.3%; and 5th: >11.5% and covariates (gender, BMI, blood pressure, insulin daily dose, lipids, statin use, diabetes duration on dyslipidemia were studied in 1275 individuals from the Brazilian multi-centre type 1 diabetes study and 171 normal controls. Results Body size and blood pressure were not correlated to lipids and glycemic control. OR (99% CI for high-LDL were 2.07 (1.21-3.54 and 2.51 (1.46-4.31, in the 4th and 5th HbA1c quintiles, respectively. Hypertriglyceridemia increased in the 5th quintile of HbA1c, OR 2.76 (1.20-6.37. OR of low-HDL-cholesterol were 0.48 (0.24-0.98 and 0.41 (0.19-0.85 in the 3rd and 4th HbA1c quintiles, respectively. HDL-cholesterol correlated positively (0.437 with HbA1c in the 3rd quintile. HDL-cholesterol and insulin dose correlated inversely in all levels of glycemic control. Conclusions Correlation of serum lipids with HbA1c is heterogeneous across the spectrum of glycemic control in type 1 diabetes individuals. LDL-cholesterol and triglycerides worsened alongside HbA1c with distinct thresholds. Association of lower HDL-cholesterol with higher daily insulin dose is consistent and it points out to a role of exogenous hyperinsulinemia in the pathophysiology of the CVRF clustering. These data suggest diverse pathophysiological processes depending on HbA1c, refuting a unified explanation for cardiovascular risk in type 1

  6. Trypanosoma brucei gambiense group 1 is distinguished by a unique amino acid substitution in the HpHb receptor implicated in human serum resistance.

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    Rebecca E Symula

    Full Text Available Trypanosoma brucei rhodesiense (Tbr and T. b. gambiense (Tbg, causative agents of Human African Trypanosomiasis (sleeping sickness in Africa, have evolved alternative mechanisms of resisting the activity of trypanosome lytic factors (TLFs, components of innate immunity in human serum that protect against infection by other African trypanosomes. In Tbr, lytic activity is suppressed by the Tbr-specific serum-resistance associated (SRA protein. The mechanism in Tbg is less well understood but has been hypothesized to involve altered activity and expression of haptoglobin haemoglobin receptor (HpHbR. HpHbR has been shown to facilitate internalization of TLF-1 in T.b. brucei (Tbb, a member of the T. brucei species complex that is susceptible to human serum. By evaluating the genetic variability of HpHbR in a comprehensive geographical and taxonomic context, we show that a single substitution that replaces leucine with serine at position 210 is conserved in the most widespread form of Tbg (Tbg group 1 and not found in related taxa, which are either human serum susceptible (Tbb or known to resist lysis via an alternative mechanism (Tbr and Tbg group 2. We hypothesize that this single substitution contributes to reduced uptake of TLF and thus may play a key role in conferring serum resistance to Tbg group 1. In contrast, similarity in HpHbR sequence among isolates of Tbg group 2 and Tbb/Tbr provides further evidence that human serum resistance in Tbg group 2 is likely independent of HpHbR function.

  7. The hematologic characteristics of sickle cell anemia bearing the Bantu haplotype: the relationship between G gamma and HbF level.

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    Nagel, R L; Rao, S K; Dunda-Belkhodja, O; Connolly, M M; Fabry, M E; Georges, A; Krishnamoorthy, R; Labie, D

    1987-04-01

    Previous work has demonstrated that the HbS gene has appeared and expanded three times in Africa in three separate geographic locations and that these three distinct mutational events can be identified by linked DNA polymorphic sites (haplotypes) surrounding the abnormal gene. We have reported that the Senegalese and Beninian haplotypes differ in G gamma expression, mean percentage of HbF, and percentage of dense cells. We now report on the third haplotype, the Bantu, and find that it has intermediate features, namely, the high mean percentage of HbF and low percentage of dense cells associated with the Senegalese haplotype, but with a low percentage of G gamma expression similar to the Beninian haplotype. The distribution of percent HbF is quite different from Senegal haplotype-bearing sickle cell anemia patients since it covers a much wider range. The low G gamma expression is also different from the Beninians since it contains a significant and unique cluster of individuals with lower than 38% G gamma. Interestingly, among the Bantu there is a strong correlation between HbF levels and G gamma expression, which is not seen with the other haplotypes. These findings open the possibility that among the Bantu haplotype-bearing individuals two chromosomal types exist that define different levels of G gamma and HbF expression. Further structural exploration of these two potential subhaplotypes is needed.

  8. Diabetes mellitus: The long way of standardization of HbA1c to the level of highest metrological order

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    Kaiser, Patricia

    2011-01-01

    Full Text Available Glycated haemoglobin (HbA1c measurements are used in clinical studies and for the management of diabetic patients. Various efforts were made to standardize the HbA1c measurements with consensus standards and standards based on a reference measurement procedure with external calibration. According to ISO 17511 a standard should meet highest accuracy possible, have a defined uncertainty of measurement and the calibration should be traceable to SI units. For HbA1c this has been realized using a LC-ID-MS procedure based on the existing reference measurement procedure.

  9. 妊娠期糖尿病产妇血液HbA1 c水平与产科不良症状发生率关系的研究%Study on the Relationship between the HbA1c Level and Obstetric Complications in Gestational Dia-betes Women

    Institute of Scientific and Technical Information of China (English)

    崔风云

    2015-01-01

    Objective To investigate the relationship between the glycosylated hemoglobin(HbA1c) level and obstetric complications in gestational diabetes women(GDM).Methods A total of 122 patients with GDM (24-28 weeks in pregnancy) were collected as the GDM group from Jun.2012 to Jun.2014 in depart-ment of Obstetric Qingdao Jiaozhou Central Hospital,GDM group were given health education,diet guide, physical exercise,and insulin when necessary to strengthen the blood glucose control.100 healthy pregnant women were selected during the same period as the control group.The serum HbA1c level was tested at admis-sion into study and before the termination of pregnancy.The obstetric complications of the two groups were compared.Results ①At study entry, the HbA1c level of GDM group and control group were ( 6.32 ± 0.30)% and (5.31 ±0.60)%,HbA1c level of GDM group was significantly higher than that of control group (P6.5% were significantly higher than patients whose HbA1c≤6.5% [26.5%(9/34) vs 5.7%(5/88),20.6%(7/34) vs 6.8%(6/88), 26.5%(9/34) vs 9.1%(8/88),32.4%(11/34) vs 7.9%(7/88),P<0.05 or P<0.01].Conclusion GDM can increase the risk of obstetric complications during pregnancy.Intensive blood glucose control during pregnancy can effectively reduce the incidence of obstetric complications .%目的:探讨妊娠期糖尿病(GDM)患者糖化血红蛋白(HbA1c)水平变化与产科不良并发症发生的关系。方法收集2012年6月至2014年6月青岛市胶州中心医院产科收治的GDM患者(孕周在24~28周)122例作为GDM组,对GDM组进行健康教育、指导饮食、体育锻炼等,必要时应用胰岛素,强化血糖控制治疗。选取同期健康妊娠妇女100例作为对照组。分别测定入组时及终止妊娠前血HbA1c水平,比较两组产科不良症状发生率。结果①GDM组入组时HbA1c水平为(6.32±0.30)%,对照组HbA1c水平为(5.31±0.60)%,GDM组 HbA1c水平显著高于对照组(P <0.05);经

  10. Effects of ginger (Zingiber officinale) on plasma glucose level, HbA1c and insulin sensitivity in type 2 diabetic patients.

    Science.gov (United States)

    Mahluji, Sepide; Attari, Vahide Ebrahimzade; Mobasseri, Majid; Payahoo, Laleh; Ostadrahimi, Alireza; Golzari, Samad E J

    2013-09-01

    The present study was aimed to evaluate the effects of Zingiber officinale on some biochemical parameters in type 2 diabetic (DM2) patients. In a randomized double-blind placebo controlled trial, 64 patients with DM2 were assigned to ginger or placebo groups (receiving 2 g/d of each). A 3 d diet record, anthropometric measurements and concentrations of fasting blood glucose (FPG), HbA1c, lipid profile (including total cholesterol, triglyceride, low density lipoprotein and high density lipoprotein) and also the homeostasis model assessment (HOMA) and quantitative insulin-sensitivity check index (QUICKI) were determined before and after 2 months of intervention. Ginger supplementation significantly lowered the levels of insulin (11.0 ± 2.3 versus 12.1 ± 3.3; p = 0.001), LDL-C (67.8 ± 27.2 versus 89.2 ± 24.9; p = 0.04), TG (127.7 ± 43.7 versus 128.2 ± 37.7; p = 0.03) and the HOMA index (3.9 ± 1.09 versus 4.5 ± 1.8; p = 0.002) and increased the QUICKI index (0.313 ± 0.012 versus 0.308 ± 0.012; p = 0.005) in comparison to the control group; while, there were no significant changes in FPG, TC, HDL-C and HbA1c (p > 0.05). In summary, ginger supplementation improved insulin sensitivity and some fractions of lipid profile in DM2 patients. Therefore it may be considered as a useful remedy to reduce the secondary complications of DM2.

  11. Change in HbA1c levels between the age of 8 years and the age of 12 years in Dutch children without diabetes: the PIAMA birth cohort study.

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    Hanneke Jansen

    Full Text Available HbA1c is associated with cardiovascular risk in persons without diabetes and cardiovascular risk accumulates over the life course. Therefore, insight in factors determining HbA1c from childhood onwards is important. We investigated (lifestyle determinants of HbA1c at age 12 years and the effects of growth on change in HbA1c and the tracking of HbA1c between the age of 8 and 12 years.Anthropometric measurements were taken and HbA1c levels were assessed in 955 children without diabetes aged around 12 years participating in the PIAMA birth cohort study. In 363 of these children HbA1c was also measured at age 8 years. Data on parents and children were collected prospectively by questionnaires.We found no significant association between known risk factors for diabetes and HbA1c at age 12 years. Mean(SD change in HbA1c between ages 8 and 12 years was 0.6(0.7 mmol/mol per year (or 0.1(0.1 %/yr. Anthropometric measures at age 8 and their change between age 8 and 12 years were not associated with the change in HbA1c. 68.9% of the children remained in the same quintile or had an HbA1c one quintile higher or lower at age 8 years compared to age 12 years.The lack of association between known risk factors for diabetes and HbA1c suggest that HbA1c in children without diabetes is relatively unaffected by factors associated with glycaemia. HbA1c at age 8 years is by far the most important predictor of HbA1c at age 12. Therefore, the ranking of HbA1c levels appear to be fairly stable over time.

  12. Haematological parameters and HbA2 levels in beta-thalassaemia trait with coincident iron deficiency.

    Science.gov (United States)

    Madan, N; Sikka, M; Sharma, S; Rusia, U

    1998-07-01

    Iron status was estimated in 463 heterozygous beta-thalassaemics to delineate the effect of iron deficiency (ID) on the haematological parameters and expression of HbA2 in these patients. One hundred and twenty six (27.2%) traits had coincident ID. These iron deficient traits had a significantly (p or = 3.5%) in all except one patient. However, mean HbA2/cell was significantly (p < 0.05) lower in traits with ID. The effect of ID in BTT was apparent with significant lowering of haemoglobin concentration and increased prevalence of anaemia. Iron therapy is warranted in iron deficient traits and would help in significantly raising their haemoglobin concentration. Elevation in HbA2 values was striking and could be used with reliability for diagnosis of BTT even in the presence of ID.

  13. Investigation of RBC Indices and HbA2 Levels in Parents of Beta-Thalassemia Patients: Impacts on Premarital Genetic Counseling

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    Mina Izadyar

    2007-06-01

    Full Text Available Objective: This study was designed to investigate RBC indices and HbA2 levels in parents of major beta-thalassemia patients to detect possible silent beta- thalassemia carriers and examine its potential impact on the premarital genetic counseling.Materials and Methods: This cross sectional study was performed at Children Medical Center from 2004 to 2006. After genetic counseling and getting informed consent, peripheral blood sampling was carried out on 185 carrier parents of regularly blood transfused thalassemia children. Then RBC indices and HbA2 concentration were measured. Samples with MCV and MCH higher than and/or HbA2 lower than cut off values were rechecked. Results: In one case, MCV and MCH indices were within the limits defined for non beta- thalassemia carriers. Furthermore, four other cases were found to have decreased values of MCV and MCH but normal HbA2 levels.Conclusion: About 3% of beta-thalassemia carriers in our country may potentially be missed using current screening methods. Further studies are required to assess the need for presenting a new threshold for thalassemia carrier screening. Defining the causative mutations using molecular methods would pave the way for establishing a protocol for a premarital screening program in conditions when one of couples is a confirmed carrier.

  14. Impact of laparoscopic sleeve gastrectomy and laparoscopic gastric bypass on HbA1c blood level and pharmacological treatment of type 2 diabetes mellitus in severe or morbidly obese patients. Results of a multicenter prospective study at 1 year.

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    Nocca, David; Guillaume, Fabre; Noel, Patrick; Picot, Marie Christine; Aggarwal, Rajesh; El Kamel, Moez; Schaub, Roxanne; de Seguin de Hons, Charles; Renard, Eric; Fabre, Jean Michel

    2011-06-01

    Gastric bypass (GBP) has proved its efficacy 30 years ago in the management of diabetes mellitus (T2DM) for severe obese patients. More recently, interesting results have been published after sleeve gastrectomy (SG) in the same indication. Between 2005 and 2008, three bariatric centers have prospectively collected the data of T2DM patients treated by laparoscopic gastric bypass (LGBP) or laparoscopic sleeve gastrectomy (LSG). Effects on hemoglobin A1c (HbA1c), pharmacological treatment and excess weight loss after 1 year of surgery have been analyzed. All patients (35 LGBP and 33 LSG) were treated with oral anti-diabetics (OAD) or insulin before surgery (32 OAD and three insulin in LGBP group and 27 OAD and six insulin in LSG group). The average body mass index (BMI) in the LGBP group was 47.9 and 50.6 kg/m² in the LSG group. At 1 year after surgery, the average HbA1c lost was 2,537 in the GBP group and 2,175 in the SG group. T2DM had resolved (withdrawal of pharmacological treatment) in 60% of the LGBP group and 75.8% of the LSG group. Reduced use of pharmacological therapy was noted in 31.42% of the LGBP group and 15.15% of the LSG group. Percentage excess weight loss and BMI lost were 56.35% and 29.75% in the LGBP group and 60.11% and 29.80% in the LSG group, respectively. During short-term follow-up, the impact on regulation of HbA1c blood level of LGBP or LSG is important. At 1 year after surgery, LSG seems to be as effective as LGBP for the management of T2DM in severely obese patients.

  15. Relationship between Hb and HbA1c in Japanese adults: an analysis of the 2009 Japan Society of Ningen Dock database.

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    Takahashi, Eiko; Moriyama, Kengo; Yamakado, Minoru

    2014-06-01

    We investigated the effect of Hb on HbA1c levels in 265,427 Japanese individuals. The divergence between fasting plasma glucose (FPG) and HbA1c levels increased with lower Hb, resulting in HbA1c levels that were higher in relation to than the FPG levels. Similarly, the correlation between FPG and HbA1c levels, stratified by Hb, weakened as Hb decreased. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  16. Relationship Between HbAlc Levels and Cerebral Arterial Lesions in Patients With Ischemic Stroke With Different Glucose Metabolism%不同糖代谢状况缺血性脑卒中患者HbAlc水平与脑动脉病变程度的关系

    Institute of Scientific and Technical Information of China (English)

    詹红艳; 杨红英; 袁莉; 牛春华; 王丽英; 陈丽丽

    2015-01-01

    ObjectiveTo study the relationship between the level of glycosylated hemoglobin and cerebral arterial lesions in patients with ischemic stroke with different glucose metabolism.Methods 120 cases of ischemic stroke patients were chosen from June 2013 to June 2015 in Tangshan workers hospital, according to glucose metabolism is divided into normal group (n=20) and abnormal group (n=65), diabetes group (n=35). To analysis of HbA1c level, risk factors for cerebral artery disease, cerebral artery lesion sites and count of the three groups were compared. ResultsThere were no significant differences in gender, hypertension, smoking, TG, Hcy among the three groups (P>0.05), the differences were statistically signiifcant with alcohol, coronary heart disease, TC, HDL-C, HbAlc, LDL-C In the three groups of patients (P<0.05). In the patients with abnormal glucose metabolism and diabetes mellitus, cerebral blood vessel stenosis was dominated by moderate and severe cerebral artery stenosis, and the main was the main and the most affected (P<0.05).Conclusion Different levels of glucose metabolism affect cerebral vascular lesions and HbAlc level of cerebral artery stenosis, the location of the lesion and lesion count is affect brain artery disease are important risk factors.%目的研究不同糖代谢状况缺血性脑卒中患者的糖化血红蛋白水平与脑动脉病变程度的关系。方法选取2013年6月~2015年6月唐山市工人医院收治的缺血性脑卒中患者120例,根据糖代谢情况分为正常组(n=20)、异常组(n=65)、糖尿病组(n=35),比较三组的一般资料、分析HbAlc水平、脑动脉病变危险因素、脑动脉病变部位及支数。结果三组患者性别、高血压、吸烟、TG、Hcy等方面比较,差异无统计学意义(P>0.05),三组患者中饮酒、冠心病、HbAlc、TC、HDL-C、LDL-C比较,差异有统计学意义(P<0.05);三组患者中,正常组脑血管狭窄以轻度为主,

  17. Dietary fiber intake is associated with HbA1c level among prevalent patients with type 2 diabetes in Pudong New Area of Shanghai, China.

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    Junyi Jiang

    Full Text Available BACKGROUND: Dietary factors play an important role in glycemic control in diabetic patients. However, little is known about their effects among Chinese diabetic patients, whose diets are typically abundant in fiber and high in glycemic index (GI values. METHODOLOGY/PRINCIPAL FINDINGS: 934 patients with type 2 diabetes and 918 healthy volunteers from Pudong New Area, Shanghai, China, were interviewed during the period of Oct-Dec, 2006 to elicit demographic characteristics and lifestyle factors. Dietary habits were assessed using a validated food frequency questionnaire. Anthropometric measurements, bio-specimen collection and biochemical assays were conducted at the interview according to a standard protocol. In this population, diabetic patients consumed lower levels of energy and macronutrients but had higher levels of fasting plasma glucose (FPG, glycolated hemoglobin A1c (HbA1c, triglyceride and body mass index than healthy adults. While the average consumption levels of the nutrients among diabetic patients did not vary along duration of the disease, the average levels of FPG and HbA1c increased with increasing duration. Regardless of diabetes duration, HbA1c level was observed lower in patients having a higher fiber or lower GI intake. Compared with those with the lowest tertile intake of fiber, the adjusted odds ratios (ORs for poor glycemic control reduced from 0.75 (95%CI: 0.54-1.06 to 0.51 (95%CI: 0.34-0.75 with increasing tertile intake (P for trend <0.001. CONCLUSIONS: Dietary fiber may play an important role in reducing HbA1c level. Increasing fiber intake may be an effective approach to improve glycemic control among Chinese diabetic patients.

  18. Extreme Levels of HbA1c Increase Incident ESRD Risk in Chinese Patients with Type 2 Diabetes: Competing Risk Analysis in National Cohort of Taiwan Diabetes Study.

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    Li-Na Liao

    Full Text Available Whether HbA1c is a predictor of end-stage renal disease (ESRD in type 2 diabetes patients remains unclear. This study evaluated relationship between HbA1c and ESRD in Chinese patients with type 2 diabetes.Patients aged ≥ 30 years who were free of ESRD (n = 51 681 were included from National Diabetes Care Management Program from 2002-2003. Extended Cox proportional hazard model with competing risk of death served to evaluate association between HbA1c level and ESRD.A total of 2613 (5.06% people developed ESRD during a follow-up period of 8.1 years. Overall incidence rate of ESRD was 6.26 per 1000 person-years. Patients with high levels of HbA1c had a high incidence rate of ESRD, from 4.29 for HbA1c of  6.0%-6.9% to 10.33 for HbA1c ≥ 10.0% per 1000 person-years. Patients with HbA1c < 6.0% particularly had a slightly higher ESRD incidence (4.34 per 1000 person-years than those with HbA1c  of 6.0%-6.9%. A J-shaped relationship between HbA1c level and ESRD risk was observed. After adjustment, patients with HbA1c < 6.0% and ≥ 10.0% exhibited an increased risk of ESRD (HR: 1.99, 95% CI: 1.62-2.44; HR: 4.42, 95% CI: 3.80-5.14, respectively compared with those with HbA1c of 6.0%-6.9%.Diabetes care has focused on preventing hyperglycemia, but not hypoglycemia. Our study revealed that HbA1c level ≥ 7.0% was linked with increased ESRD risk in type 2 diabetes patients, and that HbA1c < 6.0% also had the potential to increase ESRD risk. Our study provides epidemiological evidence that appropriate glycemic control is essential for diabetes care to meet HbA1c targets and improve outcomes without increasing the risk to this population. Clinicians need to pay attention to HbA1c results on diabetic nephropathy.

  19. One center in Brussels has consistently had the lowestHbA1c values in the 4 studies (1994-2009) by the HvidoereInternational Study Group on Childhood Diabetes: What arethe "recipes"?

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    Harry Dorchy

    2015-01-01

    The principal aims of therapeutic management of thechild, adolescent and adult with type 1 diabetes areto allow good quality of life and to avoid long-termcomplications (retinopathy, neuropathy, nephropathy,cardiovascular disease, etc. ) by maintaining bloodglucose concentrations close to normal level. Glycatedhemoglobin levelsHbA1c) provide a good criterionof overall glycemic control. The Hvidoere Study Group(HSG) on Childhood Diabetes, founded in 1994, is aninternational group representing about twenty highlyexperienced pediatric centers from Europe, NorthAmerica, Japan and Australia. Four internationalcomparisons of metabolic control (1995, 1998, 2005,2009) have been performed. The one center that hasconsistently had the lowest HbA1c values (approximate7.3% or 56.3 mmol/mol) is my center in Brussels. Thisis more often obtained with a twice-daily free-mixedregimen with additional supplemental fast insulins adhoc. The so-called "Dorchy's recipes" are summarized.The conclusion is that the number of daily insulininjections, 2 or ≥ 4, or the use of pumps, by itselfdoes not necessarily give better results. Intensifiedtherapy should not depend upon the number of insulindoses per day, by syringe, pen or pump but rathershould be redefined as to intent-to-treat ascertainment(i.e. , goals). When there are no mutually agreed upongoals for BG and/or HbA1c, when there is insufficienteducation and psychosocial support by the medicalteam or at home, there is likely to be poor outcomes,as shown by the HSG. One of our recipes is not tosystematically replace rapid-acting human insulins byfast-acting analogues. Because the multicenter studiesof the HSG, performed in developed countries withoutfinancial restriction, show that treatment of childhooddiabetes is inadequate in general and that levels ofHbA1c are very different, diabetes treatment teamsshould individually explore the reasons for failure,without any prejudice or bias. Any dogmatism must

  20. Survey on the reference values of HbA2 and HbA for healthy adults in Nanning%南宁地区健康成人HbA2和HbA参考范围调查

    Institute of Scientific and Technical Information of China (English)

    李友琼; 劳忠婵; 覃桂芳; 黄慧嫔; 阳文辉

    2012-01-01

    目的 建立广西南宁地区健康成人血红蛋白(Hb)A2和HbA参考范围.方法 采用Capillarys2毛细管电泳仪检测810例健康成人Hb,以95%参考区间(x±1.96s)建立参考范围.随机选择112例受试对象进行验证试验.结果 男、女HbA2检测结果分别为(2.79±0.24)%和(2.70±0.23)%,组间比较差异有统计学意义(P<0.01);HbA检测结果分别为(97.14±0.28)%和(97.17±0.39)%,组间比较差异无统计学意义(P>0.05).不同年龄组HbA2和HbA检测结果差异无统计学意义(P>0.05).HbA2和HbA参考范围分别为2.3%~3.3%、96.4%~97.9%.112例验证标本中,仅1例结果不适于该参考范围.结论 本研究建立的参考范围适用于该地区绝大部分健康成人.%Objective To establish the reference values of hemoglobin(Hb) A2(HbA2) and HbA for healthy adults in Nanning. Methods 810 cases of healthy adults were detected for hemoglobin by Capillarys2 Electrophoresis. Reference values were con, structed according to 95% reference range. 112 randomly selected subjects were enrolled for verification experiment. Results HbA2 levels in males and females were (2. 79 ± 0. 24)% and (2. 70 ± 0. 23)% respectively with statistical difference(P< 0. 01). HbA lev, els were (97. 14 ± 0. 28)% and (97. 17 ± 0. 39)% in males and females respectively without significant difference(P>0. 05). There was no statistical difference of HbA2 and HbA levels between different age groups(P>0. 05). Reference values of HbA2 and HbA ranged from 2. 3% to 3. 3% and from 96. 4% to 97. 9% respectively. Among all the cases of verification samples,only 1 case was unfit for the reference values. Conclusion Reference values of HbA2 and HbA constructed in the research might be fit for most healthy adults in nanning.

  1. Treatment intensification without improved HbA1c levels in children and adolescents with Type 1 diabetes mellitus.

    Science.gov (United States)

    Sildorf, S M; Hertel, N T; Thomsen, J; Fredheim, S; Hastrup, H; Pipper, C; Hertz, B; Svensson, J

    2016-04-01

    To examine trends in diabetes treatment in Danish children and adolescents with Type 1 diabetes mellitus, comparing treatment intensity with metabolic outcomes in the population, and to describe the challenges of population-based registries in a clinical setting with rapidly changing treatment methods. This observational study is based on the Danish national population registry of childhood diabetes, which includes 99% of children diagnosed with Type 1 diabetes before the age of 15 years. We included 4527 people diagnosed between 2000 and 2012. Self-monitored blood glucose measurements, insulin injections/boluses, treatment method and metabolic control quantifications were analysed and adjusted for the effects of gender and ethnicity, the combined effect of age, visit year and duration, and for the random effects of individual and hospital settings. Treatment was intensified via an increasing number of self-monitored blood glucose measurements and injections/boluses. More than six injections/boluses and an increased number of self-monitored blood glucose measurements were significantly associated with lower metabolic control. No reduction, however, in the overall mean HbA1c concentration was observed between 2005 [66 mmol/mol (8.2%)] and 2012 [65 mmol/mol (8.1%)]. Changed registration practices in 2009 introduced artificial jumps in data. Intensifying treatment alone does not lead to improved metabolic control in the overall population despite the appearance of lower HbA1c in individuals with a greater number of self-monitored blood glucose measurements and injections/boluses. The contradictory results reflect difficulties in using observational studies to predict results of intervention in the individual. Data collected from population-based registries need to be adjusted continuously to reflect changes in care. © 2015 Diabetes UK.

  2. Long-term effects of a diet loosely restricting carbohydrates on HbA1c levels, BMI and tapering of sulfonylureas in type 2 diabetes: a 2-year follow-up study.

    Science.gov (United States)

    Haimoto, Hajime; Iwata, Mitsunaga; Wakai, Kenji; Umegaki, Hiroyuki

    2008-02-01

    The aim was to assess the long-term effect of a loose restriction of carbohydrate intake (carbohydrate-reduced diet: CARD) compared to a conventional diet (CD) in type 2 diabetes. One hundred and thirty-three type 2 diabetic outpatients followed the CD (n=57, 1734+/-410 kcal, carbohydrate:protein:fat ratio=57:16:26) or CARD (n=76, 1773+/-441 kcal, carbohydrate:protein:fat ratio=45:18:33) according to their own will, and were followed up for 2 years. Glycemic control, body mass index (BMI), serum cholesterols and dose of antidiabetic drugs were assessed at baseline and after 1 and 2 years. At baseline, hemoglobin A1c (HbA1c) and BMI levels were 7.1+/-1.0% and 24.2+/-2.9, respectively, in the CD group, and 7.4+/-1.1% and 25.1+/-3.4 in the CARD group, showing no significant differences. During the 2-year follow-up period, HbA1c levels were significantly improved in the CARD group (CD: 7.5+/-1.3%, CARD: 6.7+/-0.6%, Pdiet for type 2 diabetes.

  3. [Effects of applying behavior modification to improve HbA1C levels in a diabetic patient].

    Science.gov (United States)

    Chen, Wen-Chun; Huang, You-Rong; Lin, Chiu-Chu

    2010-04-01

    Diabetes is a chronic disease. To prevent and delay complications, diabetic patients must adjust their lifestyle as part of a comprehensive approach to disease control. Diabetic patients must be able to self-manage their disease and establish healthy habits in their daily routine. In this study, prior to intervention, the subject was unable to control her diet, do exercise, check sugars properly or integrate disease management effectively into her daily routine. By applying self-regulation theory through the keeping of a diary for sugar and daily activity self-monitoring, she became able to self-assess the causes of poor disease control. Such further facilitated her setting goals and developing strategies to link her habits with disease management. When failing to achieve goals even after execution, she could consider the factors contributing to the failure and modify her behaviors, goals and/or strategies accordingly. We helped this patient learn behavior modification methods in order to achieve her goal of better HbA(1)C control. This case example may help clinical nursing educators move beyond the confines of the traditional one-way educational model to guide diabetic patients to achieve good sugar control. We hope our findings help many chronic disease sufferers achieve self-management objectives in order to assume greater self-care responsibilities.

  4. Changes in HbA1c and circulating and adipose tissue androgen levels in overweight‐obese women with polycystic ovary syndrome in response to electroacupuncture

    Science.gov (United States)

    Maliqueo, M.; Soligo, M.; Protto, V.; Manni, L.; Jerlhag, E.; Kokosar, M.; Sazonova, A.; Behre, C. J.; Lind, M.; Ohlsson, C.; Højlund, K.; Benrick, A.

    2016-01-01

    Summary Aim Insulin sensitivity is ~40% lower in women with polycystic ovary syndrome (PCOS) than in controls. We tested the hypothesis that 5 weeks of electroacupuncture treatment improves glucose regulation and androgen levels in overweight/obese women with PCOS. Material and Methods Seventeen women with PCOS, aged 18 to 38 years, with a body mass index (BMI) ≥25 kg/m2 and diagnosed with PCOS were included in this experimental and feasibility study and subjected to five weeks of electroacupuncture treatments three times/week. The primary outcome was changes in whole‐body glucose homeostasis measured by euglycemic hyperinsulinemic clamp before and after the intervention. Secondary outcome were changes in HbA1c, circulating catecholamines, adipocyte size and adipose tissue expression of sex steroids and nerve growth factor (NGF). Results No significant change in glucose homeostasis was observed, but HbA1c decreased by 9.5% (p = 0.004), circulating testosterone decreased by 22% (p = 0.0007) and dihydrotestosterone decreased by 12% (p = 0.007). The two vagal activity markers of plasma serotonin levels and the dopamine metabolite homovanillic acid decreased by 21% (p = 0.027) and 20% (p = 0.011), respectively. Adipose tissue concentrations of testosterone decreased by 18% (p = 0.049), and androstenedione decreased by 13% (p = 0.035), and mature NGF/proNGF ratio, a marker of sympathetic activity, increased (p = 0.04). These changes occurred without changes in anthropometrics. Conclusion Five weeks of electroacupuncture treatment improves HbA1c and circulating and adipose tissue androgens in women with PCOS. This effect is mediated, at least in part, via modulation of vagal activity and adipose tissue sympathetic activity. Based on these findings, we have recently initiated a randomized controlled study (NTC02647827). PMID:28090348

  5. Correlation of HbA1c levels with severity, prognosis and recurrence of patients with cerebral infarction%糖化血红蛋白水平与脑梗死严重程度、预后及复发的相关性研究

    Institute of Scientific and Technical Information of China (English)

    王锐; 周国霞

    2013-01-01

    Objective To investigate the correlation of HbAlc levels with severity,prognosis and recurrence of patients with cerebral infarction during the acute stage and a year after onset.Methods Prospective cohort study was performed in the clinical data of 228 inpatients with acute cerebral infarction,admitted to our hospital from September 2006 to September 2010.Levels of glycosylated hemoglobin (HbA1c),fasting plasma glucose (FBG),systolic pressure (SBP),diastolic blood pressure (DBP),cholesterol (TC),triglyceride (TG),low density lipoprotein (LDL-C) and high density lipoprotein cholesterol (HDL-C) at the acute stage were detected; the living activity scale (Barthel index,BI) was conducted.According to the levels of HbA1c and FBG at admission,the patients were divided into four groups:groups of HbA1c≥6.1 and FBG≥6.1,group of HbA1c≥6.1 and FBG<6.1,group of HbA1c<6.1 and FBG≥6.1,and group of HbA1c<6.1 and FBG<6.1.Single-factor analysis of variance was employed to analyze the differences of all the indexes at acute stage between each two groups;correlations of BI scores at acute stage and one year after onset with other indexes were explored; t-test was employed to analyze the differences of all the indexes between patients with and without relapsed cerebral infarction.Results During the acute stage of cerebral infarction,significant differences were noted on levels of TC,TG,LDL-C,SBP and BI scores between each groups (P<0.05); BI scores were negatively correlated with HbA1c and SBP levels.One year after the onset,the BI scores were negatively correlated with HbA1c,FBG,SBP levels at the acute stage,and HbA1c and FBG levels one year after onset,respectively.Overall recurrence rate was 5.88% after a year of follow up; the HbA1c,FBG and TG levels at acute stage,and FBG and PBG levels one year after onset showed significant differences between patients with and without relapsed cerebral infarction (P<0.05).Conclusion Patients with increased HbA1c and FBG

  6. 脑梗死患者血糖及糖化血红蛋白检测的临床价值%Clinical significance of blood glucose and HbA1c level in patients with cerebra infarction

    Institute of Scientific and Technical Information of China (English)

    陈新瑶

    2013-01-01

      目的:探讨血糖和糖化血红蛋白(HbA1c)水平与脑梗死的相关性.方法:选择急性脑梗死患者169例,测定静脉空腹血糖、HbA1c水平,比较不同神经功能损坏程度、病灶大小、预后情况与空腹血糖、HbA1c的相关性.结果:脑神经功能受损重度组血糖水平显著高于轻度组(P0.05);脑神经功能不同程度受损、病灶不同大小、预后不同效果各组间的HbA1c水平差异均有统计学意义(P0.05);brain function damaged to varying degrees,different sizes,different prognostic effect of lesions among different HbA1c level differences were statistically significant(P<0.05).Conclusion The blood glucose,HbA1c levels and the severity of cerebral infarction is closely related to the level of HbA1c,high blood sugar,is not conducive to the prognosis of cerebral infarction.

  7. Medication Adherence Mediates the Association between Type D Personality and High HbA1c Level in Chinese Patients with Type 2 Diabetes Mellitus: A Six-Month Follow-Up Study

    Directory of Open Access Journals (Sweden)

    Xuemei Li

    2017-01-01

    Full Text Available Aims. To examine the association between Type D personality and HbA1c level and to explore the mediating role of medication adherence between them in patients with type 2 diabetes mellitus (T2DM. Methods. 330 patients went on to complete a self-report measure of medication adherence and the HbA1c tests. Chi-square test, T test, Ordinary Least Square Regression (OLS, and Recentered Influence Function Regression (RIF were employed. Results. Patients with Type D personality had significantly higher HbA1c value (P0.1. On the other hand, when Type D personality was operationalized as a continuous variable, only SI trait was associated with HbA1c level (P<0.01. When NA, SI, and NA×SI term together were entered into regression, only SI was not related to HbA1c level. Furthermore, medication adherence had a significant mediation effect between Type D personality and HbA1c, accounting for 54.43% of the total effect. Conclusion. Type D personality was associated with HbA1c in direct and indirect ways, and medication adherence acted as a mediator role.

  8. Good for the group? Explaining apparent group-level selection

    NARCIS (Netherlands)

    Smallegange, I.M.; Egas, M.

    2015-01-01

    The idea that group selection can explain adaptive trait evolution is still controversial. Recent empirical work proposes evidence for group-level adaptation in a social spider, but the findings can also be explained from an individual-level perspective. The challenge remains to identify situations

  9. The incidence of severe hypoglycaemia in pregnant women with type 1 diabetes mellitus can be reduced with unchanged HbA1c levels and pregnancy outcomes in a routine care setting

    DEFF Research Database (Denmark)

    Ringholm, Lene; Secher, A L; Pedersen-Bjergaard, U;

    2013-01-01

    To investigate whether the incidence of severe hypoglycaemia in pregnant women with type 1 diabetes can be reduced without deteriorating HbA1c levels or pregnancy outcomes in a routine care setting.......To investigate whether the incidence of severe hypoglycaemia in pregnant women with type 1 diabetes can be reduced without deteriorating HbA1c levels or pregnancy outcomes in a routine care setting....

  10. Medication Adherence Mediates the Association between Type D Personality and High HbA1c Level in Chinese Patients with Type 2 Diabetes Mellitus: A Six-Month Follow-Up Study

    Science.gov (United States)

    Li, Xuemei; Gao, Min; Zhang, Shengfa; Xu, Huiwen; Zhou, Huixuan; Wang, Xiaohua; Qu, Zhiyong; Guo, Jing

    2017-01-01

    Aims. To examine the association between Type D personality and HbA1c level and to explore the mediating role of medication adherence between them in patients with type 2 diabetes mellitus (T2DM). Methods. 330 patients went on to complete a self-report measure of medication adherence and the HbA1c tests. Chi-square test, T test, Ordinary Least Square Regression (OLS), and Recentered Influence Function Regression (RIF) were employed. Results. Patients with Type D personality had significantly higher HbA1c value (P personality was operationalized as a categorical variable, SI was associated with HbA1c (P 0.1). On the other hand, when Type D personality was operationalized as a continuous variable, only SI trait was associated with HbA1c level (P personality and HbA1c, accounting for 54.43% of the total effect. Conclusion. Type D personality was associated with HbA1c in direct and indirect ways, and medication adherence acted as a mediator role.

  11. Is Distributed Cognition Group Level Cognition?

    Directory of Open Access Journals (Sweden)

    Ludwig Kirk

    2015-09-01

    Full Text Available This paper shows that recent arguments from group problem solving and task performance to emergent group level cognition that rest on the social parity and related principles are invalid or question begging. The paper shows that standard attributions of problem solving or task performance to groups require only multiple agents of the outcome, not a group agent over and above its members, whether or not any individual member of the group could have accomplished the task independently.

  12. Energy balance and macronutrient distribution in relation to C-reactive protein and HbA1c levels among patients with type 2 diabetes

    Directory of Open Access Journals (Sweden)

    Hiba Bawadi

    2016-05-01

    Full Text Available Background: Recently growing evidence indicates that obesity and diabetes are states of inflammation associated with elevated circulation of inflammatory mediators. Excess adiposity and oxidative stress, induced by feeding, may also lead to a state of low-grade inflammation. Objective: This study aimed at investigating energy balance and distribution in relation to low-grade inflammation among patients with type 2 diabetes. Design: A cross-sectional study included 198 male and female patients with type 2 diabetes. Patients’ weight, height, waist circumference, total body fat and truncal fat percent, energy, and macronutrient intake were measured. Venous blood specimens were collected, and levels of HbA1c and serum levels of high-sensitivity C-reactive protein (hs-CRP were determined. Results: After adjusting for covariates (body mass index, total body fat, and truncal fat, energy balance was positively correlated with hs-CRP and HbA1c. A positive energy balance was also associated with increased waist circumference and truncal fat percent (p<0.05. Total energy intake, percent energy from fat (p=0.04, and percent energy from proteins (p=0.03, but not percent energy from carbohydrates (p=0.12, were also correlated with higher hs-CRP levels among poorly glycemic-controlled patients. Conclusion: Positive energy balance is associated with elevations in hs-CRP. Increased energy intake and increased percentages of energy from fat and protein are associated with elevated hs-CRP among patients with poor glycemic control.

  13. Plasma dipeptidyl peptidase-IV activity in patients with type-2 diabetes mellitus correlates positively with HbAlc levels, but is not acutely affected by food intake

    DEFF Research Database (Denmark)

    Ryskjaer, Jakob; Deacon, Carolyn F.; Carr, Richard D;

    2006-01-01

    a standard meal test (566 kcal) was estimated. RESULTS: Mean fasting plasma DPP-IV activity (expressed as degradation of GLP-1) was significantly higher in this patient group compared with the control subjects (67.5 +/- 1.9 vs 56.8 +/- 2.2 fmol GLP-1/h (mean +/- s.e.m.); P=0.001). In the type-2 diabetic...... patients and control subjects. DESIGN: The study included two protocols. Protocol one involved 40 fasting type-2 diabetic patients (28 men); age 61 +/- 1.4 (mean +/- s.e.m.) years; body mass index (BMI) 31 +/- 0.6 kg/m(2); HbAlc 7.2 +/- 0.2%; and 20 matched control subjects (14 men) were studied. Protocol...

  14. Genome-wide association study identifies common loci influencing circulating glycated hemoglobin (HbA1c) levels in non-diabetic subjects

    DEFF Research Database (Denmark)

    An, Ping; Miljkovic, Iva; Thyagarajan, Bharat;

    2014-01-01

    Glycated hemoglobin (HbA1c) is a stable index of chronic glycemic status and hyperglycemia associated with progressive development of insulin resistance and frank diabetes. It is also associated with premature aging and increased mortality. To uncover novel loci for HbA1c that are associated...

  15. Self-knowledge of HbA1c in people with Type 2 Diabetes Mellitus and its association with glycaemic control.

    Science.gov (United States)

    Trivedi, Hina; Gray, Laura J; Seidu, Samuel; Davies, Melanie J; Charpentier, Guillaume; Lindblad, Ulf; Kellner, Christiane; Nolan, John; Pazderska, Agnieszka; Rutten, Guy; Trento, Marina; Khunti, Kamlesh

    2017-10-01

    The aim of this study was to evaluate the prevalence of accurate self-knowledge of a patient's own HbA1c level (HbA1cSK), as a component of structural education (University Hospital's of Leicester (UHL), 2013) and its association with glycaemic control. Data from the GUIDANCE study, a cross-sectional study involving 7597 participants from eight European countries was used. HbA1cSK was evaluated and compared with laboratory measured HbA1c levels (HbA1cLAB), which represented the measure of glycaemic control. Accuracy of the self-reported HbA1c was evaluated by using agreement statistical methods. The prevalence of HbA1cSK was 49.4%. Within this group, 78.3% of the participants had accurately reported HbA1cSK. There was good level of agreement between HbA1cSK and HbA1cLAB (intra-class correlation statistic=0.84, pself-knowledge of their own HbA1c level. Moreover, the participants with accurately reported HbA1cSK were found to have associated better glycaemic control. Copyright © 2017 Primary Care Diabetes Europe. Published by Elsevier Ltd. All rights reserved.

  16. HbD Punjab/HbQ India Compound Heterozygosity: An Unusual Association

    Science.gov (United States)

    Colaco, Stacy; Surve, Reema; Sawant, Pratibha; Nadkarni, Anita; Ghosh, Kanjaksha; Colah, Roshan

    2014-01-01

    Background Haemoglobinopathies are the commonest hereditary disorders in India and pose a major health problem. Both beta thalassaemia and structural haemoglobin variants are relatively common in northwestern India. Here we report a 29-year-old Sindhi female who was referred to us for a haemoglobinopathy work up and genetic counseling since her spouse was a classical beta thalassaemia carrier. Method A complete blood count was done on an automated cell counter. Haemoglobin analysis was carried out using HPLC Variant Haemoglobin Testing System. The cellulose acetate electrophoresis was carried out [pH 8.9]. Confirmation of mutations was done by automated DNA sequencing. Results HPLC analysis showed four major peaks, HbA0, a peak in the HbD window, an unknown peak [retention time 4.74 minutes] and a peak in the HbC window. The HbA2 level was 2.2%, and the HbF level was 0.7%. Cellulose acetate electrophoresis at alkaline pH, a slow moving band was seen at the HbS/D position along with a prominent band at the HbA2 position. DNA sequencing of the β and α genes showed presence of the two hemoglobin variants: Hb D [β 121GAA → CAA] and Hb Q [α 64 AAG → GAG]. The δ globin gene was normal. The additional peak in the HbC window was due to the formation of a heterodimer hybrid. Conclusion Both HbD Punjab and HbQ India are relatively common in India, but their co-inheritance has not been described in the country. This case is the third report of compound heterozygosity for HbQ India/HbD Punjab haemoglobinopathy globally and the second one from India. PMID:25408858

  17. HbD Punjab/HbQ India compound heterozygosity: An unusual association.

    Directory of Open Access Journals (Sweden)

    Stacy Colaco

    2014-11-01

    Full Text Available Background: Haemoglobinopathies are the commonest hereditary disorders in India and pose a major health problem. Both beta thalassaemia and structural haemoglobin variants are relatively common in north western India. Here we report a 29 year old Sindhi female who was referred to us for a haemoglobinopathy work up and genetic counseling since her spouse was a classical beta thalassaemia carrier. Method: A complete blood count was done on an automated cell counter. Haemoglobin analysis was carried out using HPLC Variant Haemoglobin Testing System.  The cellulose acetate electrophoresis was carried out [pH 8.9]. Confirmation of mutations was done by automated DNA sequencing. Results: HPLC analysis showed four major peaks, HbA0, a peak in the HbD window, an unknown peak [retention time 4.74 minutes] and a peak in the HbC window. The HbA2 level was 2.2% and the HbF level was 0.7%.Cellulose acetate electrophoresis at alkaline pH, a slow moving band was seen at the HbS/D position along with a prominent band at the HbA2 position. DNA sequencing of the β and α genes showed presence of the 2 hemoglobin variants :Hb D [b 121GAA à CAA] and Hb Q [a 64 AAG à GAG]. The δ globin gene was normal. The additional peak in the HbC window was due to the formation of a heterodimer hybrid. Conclusion: Both HbD Punjab and HbQ India are relatively common in India but their co-inheritance has not been described in the country. This is the second report of compound heterozygosity for HbQ India/HbD Punjab haemoglobinopathy globally, and the first one from India.

  18. Influence of HbA1c levels on platelet function profiles associated with tight glycemic control in patients presenting with hyperglycemia and an acute coronary syndrome. A subanalysis of the CHIPS Study ("Control de HIperglucemia y Actividad Plaquetaria en Pacientes con Síndrome Coronario Agudo").

    Science.gov (United States)

    Vivas, David; García-Rubira, Juan C; Bernardo, Esther; Angiolillo, Dominick J; Martín, Patricia; Calle-Pascual, Alfonso; Núñez-Gil, Iván; Macaya, Carlos; Fernández-Ortiz, Antonio

    2013-02-01

    Patients with hyperglycemia, an acute coronary syndrome and poor glycemic control have increased platelet reactivity and poor prognosis. However, it is unclear the influence of a tight glycemic control on platelet reactivity in these patients. This is a subanalysis of the CHIPS study. This trial randomized patients with hyperglycemia to undergo an intensive glucose control (target blood glucose 80-120 mg/dL), or conventional glucose control (target blood glucose <180 mg/dL). We analyzed platelet function at discharge on the subgroup of patients with poor glycemic control, defined with admission levels of HbA1c higher than 6.5%. The primary endpoint was maximal platelet aggregation following stimuli with 20 μM ADP. We also measured aggregation following collagen, epinephrine, and thrombin receptor-activated peptide, as well as P2Y12 reactivity index and surface expression of glycoprotein IIb/IIIa and P-selectin. A total of 67 patients presented HbA1c ≥ 6.5% (37 intensive, 30 conventional), while 42 had HbA1c < 6.5% (20 intensive, 22 conventional). There were no differences in baseline characteristics between groups. At discharge, patients with HbA1c ≥6.5% had significantly reduced MPA with intensive glucose control compared with conventional control (46.1 ± 22.3 vs. 60.4 ± 20.0%; p = 0.004). Similar findings were shown with other measures of platelet function. However, glucose control strategy did not affect platelet function parameters in patients with HbA1c < 6.5%. Intensive glucose control in patients presenting with an acute coronary syndrome and hyperglycemia results in a reduction of platelet reactivity only in the presence of elevated HbA1c levels.

  19. A polymorphism within the fructosamine-3-kinase gene is associated with HbA1c Levels and the onset of type 2 diabetes mellitus.

    Science.gov (United States)

    Mohás, M; Kisfali, P; Baricza, E; Mérei, A; Maász, A; Cseh, J; Mikolás, E; Szijártó, I A; Melegh, B; Wittmann, I

    2010-03-01

    Non-enzymatic glycation is a process, which leads to the formation of advanced glycation endproducts. These compounds are involved in the development of diabetic microvascular complications. Fructosamine-3-kinase (FN3K) is an intracellular enzyme that phosphorylates fructosamines resulting in fructosamine-3-phosphate, which subsequently decomposes to inorganic phosphate, 3-deoxyglucasone and the unmodified amine. Recently, the G900C (rs1056534) single nucleotide polymorpism (SNP) of the FN3K gene was found to be associated with the enzyme activity. The aim of the study was to investigate the impact of the SNP on clinical and biochemical features and microvascular complications of type 2 diabetes. A total of 859 type 2 diabetic subjects and 265 healthy controls were enrolled in the study and were genotyped with PCR-RFLP method. Genotype frequencies were as follows, CC: 5%, GC: 54%, GG: 41% in subjects with type 2 diabetes and CC: 6%, GC: 51%, GG: 43% in the controls. Diabetic subjects with the CC variant had lower HbA (1c) levels compared with the others (CC: 6.48+/-0.05%; GC: 7.66+/-0.09%; GG: 7.68+/-0.09%; p<0.001). Furthermore, in case of the CC allelic variant type 2 diabetes was diagnosed at a later age than in case of GC or GG variants (CC: 56.0+/-1.90 years; GC: 52.0+/-0.62 years; GG: 50.1+/-0.71 years; p<0.05). Logistic regression analysis did not reveal association between CC genotype and diabetic complications, such as diabetic nephropathy, neuropathy and retinopathy (OR=1.036, CI 95% 0.652-1.647, p=0.880; OR=0.985, CI 95% 0.564-1.721 p=0.958; OR=1.213, CI 95% 0.470-3.132, p=0.690, respectively). We conclude that the G900C polymorphism associates with the level of HbA (1c) and the onset of the disease, but not with either of the diabetic microvascular complications. J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart * New York.

  20. Performance of the Roche second generation hemoglobin A1c immunoassay in the presence of HB-S or HB-C traits.

    Science.gov (United States)

    Abadie, Jude M; Koelsch, Angela A

    2008-01-01

    Blood HbA1c determination is a powerful tool for the evaluation and management of patients with diabetes mellitus. Many HbA1c analytical methods demonstrate bias in samples from patients with hemoglobinopathies. This study evaluated the analytical performance of Roche Diagnostics' 1st and 2nd generation HbA1c assays in patients with or without hemoglobinopathies whose HbA1c levels were elevated or normal, respectively. Boronate-affinity high performance liquid chromatography (HPLC) served as the reference method. Whole blood samples were collected from 80 patients with HbS or HbC whose group mean HbA1c value was elevated and also from 80 patients without hemoglobinopathy whose HbA1c values were in the well-controlled range. Each sample was assayed for HbA1c by the Primus boronate-affinity HPLC technique and by Roche's 1st and 2nd generation immunoassays using a Cobas Integra 800 analytical system. Results by the HPLC technique were compared with the results of both Roche assays by linear regression and Bland-Altman analysis. The 1st and 2nd generation assays yielded regression lines and correlation values vs HPLC assay of y = 1.43x - 1.59; R(2) = 0.83, and y = 0.94x + 0.10; R(2) = 0.92, respectively, in the 80 patients with hemoglobinopathies. The mean difference and the +/-2SD range were greater in the 1st than in the 2nd generation assay (2.68, +/-2.07 vs -0.54, +/-0.86, respectively). The 2nd generation assay also showed better performance than the 1st generation assay in samples from the 80 patients without hemoglobinopathy. In conclusion, this study validates the accuracy of Roche's 2nd generation assay, which is substantially improved over Roche's 1st generation HbA1c assay.

  1. Changes in HbA1c and circulating and adipose tissue androgen levels in overweight-obese women with polycystic ovary syndrome in response to electroacupuncture

    DEFF Research Database (Denmark)

    Stener-Victorin, E.; Maliqueo, M.; Soligo, M

    2016-01-01

    by euglycemic hyperinsulinemic clamp before and after the intervention. Secondary outcome were changes in HbA1c, circulating catecholamines, adipocyte size and adipose tissue expression of sex steroids and nerve growth factor (NGF). RESULTS: No significant change in glucose homeostasis was observed, but HbA1c....... Adipose tissue concentrations of testosterone decreased by 18% (p = 0.049), and androstenedione decreased by 13% (p = 0.035), and mature NGF/proNGF ratio, a marker of sympathetic activity, increased (p = 0.04). These changes occurred without changes in anthropometrics. CONCLUSION: Five weeks...... of electroacupuncture treatment improves HbA1c and circulating and adipose tissue androgens in women with PCOS. This effect is mediated, at least in part, via modulation of vagal activity and adipose tissue sympathetic activity. Based on these findings, we have recently initiated a randomized controlled study (NTC...

  2. GM-CSF in sickle cell anemia patients with elevated Hb F.

    Science.gov (United States)

    Haider, M Z; Raghupathy, R; Azizieh, F; Abdelsalam, R; D'Souza, T M; Adekile, A D

    2000-01-01

    We estimated plasma GM-CSF levels in a group of 28 steady-state sickle cell anemia (SS) patients in Kuwait, using an ELISA technique. There were 24 age-matched Hb AA controls, 14 of whom were healthy while 10 were acutely ill at the time of the study. Five SS patients were also studied during 6 episodes of painful crisis. Among the SS patients, 82.1% were homozygous for the Saudi Arabia/India (SAI) haplotype with Hb F ranging from 15 to 35% and total Hb from 8.5 to 11 g/dl. Three patients (siblings) were SAI/Benin compound heterozygotes with Hb F of 9-23% and total Hb >10 g/dl. One patient each was homozygous for the Benin or the Bantu haplotype; they had Hb F <2% and total Hb of 6.6 and 7.2 g/dl, respectively. Four (14. 3%) steady-state SS patients had detectable plasma GM-CSF ranging from 75 to 1,817.6 pg/ml. These included the 2 patients with Hb F <2. 0% and 2 with the SAI/Benin compound heterozygotes with Hb F of 11 and 9%, respectively. Four (66.7%) SS patients in crisis, 6 (42.9%) healthy controls and 6 (60%) acutely ill controls had detectable plasma GM-CSF. A clearcut association of GM-CSF with Hb F level or degree of anemia in steady-state SS patients could not be established. The appearance of GM-CSF in the plasma of patients in crisis and also among control subjects raises the possibility that other factors are involved in the production of this cytokine in the subjects studied.

  3. Scalable group level probabilistic sparse factor analysis

    DEFF Research Database (Denmark)

    Hinrich, Jesper Løve; Nielsen, Søren Føns Vind; Riis, Nicolai Andre Brogaard

    2017-01-01

    Many data-driven approaches exist to extract neural representations of functional magnetic resonance imaging (fMRI) data, but most of them lack a proper probabilistic formulation. We propose a scalable group level probabilistic sparse factor analysis (psFA) allowing spatially sparse maps, component...... pruning using automatic relevance determination (ARD) and subject specific heteroscedastic spatial noise modeling. For task-based and resting state fMRI, we show that the sparsity constraint gives rise to components similar to those obtained by group independent component analysis. The noise modeling...... shows that noise is reduced in areas typically associated with activation by the experimental design. The psFA model identifies sparse components and the probabilistic setting provides a natural way to handle parameter uncertainties. The variational Bayesian framework easily extends to more complex...

  4. The interference by HbF on HbA1c (BM Test HbA1c) measurement in enzymatic method.

    Science.gov (United States)

    Nitta, Takenori; Yamashiro, Yasuhiro; Hattori, Yukio; Ezumi, Tomoko; Nishioka, Mituaki; Nakamura, Junji

    2015-09-01

    High performance liquid chromatography and immunological and enzymatic methods are known as the methods for HbA1c measurement. However, the differences in the principles of the methods may cause slight discrepancies, which become problems especially in the regions where hemoglobinopathies that have high HbF concentrations are commonly seen. In this report, we discuss the effect of HbF on the HbA1c measurement by the enzymatic method (BM Test HbA1c) which can be applied to automatic analyzer of high sample throughput. Thirty-five samples (HbF concentration: between 1.4 and 90.1%) and 68 samples (HbF concentration: <1.0%) were analyzed by high performance liquid chromatography (Tosoh G8) and BM Test HbA1c. The results by BM Test HbA1c were compared with those by the high performance liquid chromatography and International Federation of Clinical Chemistry reference method. The high and positive correlation coefficient was demonstrated between the enzymatic and International Federation of Clinical Chemistry methods for the samples with normal HbF concentration. The samples with high HbF concentration exhibited high and positive correlation coefficient, although the figure is lower than that of the normal HbF concentration group. BM Test HbA1c revealed a high and positive correlation with high performance liquid chromatography and International Federation of Clinical Chemistry reference methods. However, the HbA1c values by BM Test HbA1c and high performance liquid chromatography method were lower than those by International Federation of Clinical Chemistry reference method when the HbF concentration was high. BM Test HbA1c had some deviation from the International Federation of Clinical Chemistry reference method, while no significant interference was seen when the HbF values were less than 15%. When HbF is higher than 15%, however, the interpretation of HbA1c by BM Test may require some special consideration. © The Author(s) 2015.

  5. Intake of Vitamin and Mineral Supplements and Longitudinal Association with HbA1c Levels in the General Non-Diabetic Population--Results from the MONICA/KORA S3/F3 Study.

    Directory of Open Access Journals (Sweden)

    Sigrid Schwab

    Full Text Available Lower levels of hemoglobin A1c (HbA1c are associated with a decreased risk of cardiovascular complications in diabetic and non-diabetic individuals. The aim of the study was to longitudinally investigate the association between the use of 11 vitamins and minerals (vitamins E, C, D, B1, folic acid, carotenoids, calcium, magnesium, zinc, iron, and selenium and change in HbA1c levels over 10 years in non-diabetic individuals drawn from the general population.Baseline data were available from 4447 subjects included in the population-based "Monitoring of Trends and Determinants in Cardiovascular Diseases" (MONICA Augsburg S3 survey (1994/95. Follow-up data were derived from 2774 participants in the follow-up survey named "Cooperative Health Research in the Region of Augsburg" (KORA F3 (2004/05. Vitamin/mineral intake from supplements and medications was assessed in a personal interview, where participants were asked to bring product packages of preparations that had been ingested during the last 7 days prior to the examination. Associations between regular vitamin/mineral intake amounts and HbA1c levels measured at baseline and follow-up were investigated using generalized estimating equation models. For carotenoids, analyses were stratified by smoking status.None of the investigated nutrients except for carotenoids was significantly associated with changes in HbA1c levels after 10 years. Regular intake of carotenoids from supplements and medications in amounts > 6.8 mg/d (upper tertile was associated with an absolute -0.26% (95% CI: -0.43 to -0.08 lower increase in HbA1c levels compared with no intake of carotenoids. An inverse association was observed in those who never smoked but not in (former smokers.Larger prospective and intervention studies in non-diabetic/non-smoking individuals are needed to confirm the results and to assess whether the observed associations between carotenoid intake and change in HbA1c levels are causal. If our results are

  6. Common haplotype dependency of high G gamma-globin gene expression and high Hb F levels in beta-thalassemia and sickle cell anemia patients.

    OpenAIRE

    Labie, D; Pagnier, J.; Lapoumeroulie, C; Rouabhi, F; Dunda-Belkhodja, O; Chardin, P; Beldjord, C; Wajcman, H; Fabry, M E; Nagel, R L

    1985-01-01

    We have studied 42 homozygous beta-thalassemia patients from Algeria and 34 sickle cell anemia patients from Senegal and Benin, determining the relationship between haplotypes, Hb F, and G gamma-globin/A gamma-globin ratios. Populations selected have a high frequency of haplotype homozygotes because of consanguinity (Algeria) and geographic homogeneity (West Africa). We find in beta-thalassemia patients, that haplotype IX in haplotypic homozygotes and heterozygotes, haplotype III in heterozyg...

  7. Pengaruh ekstrak melon terhadap kadar HbCO pada tikus Wistar jantan yang dipapar asap rokok

    Directory of Open Access Journals (Sweden)

    Yuyun Erlina Susanti

    2017-01-01

    Full Text Available Background: Smoking is something that is usual for most Indonesian society, especially adult males. According to Riskesdas, smoking behavior of the population trends to increase. A previous study suggested that exposure to cigarette smoke produce CO bond to hemoglobin. Melon (Cucumis melo contains antioxidants that prevent tissue damage. Objective: Analyze differences in levels HbCO Wistar male rats in treated and untreated extracts of melon. Method: The research was a laboratory experiment with the post test control group design and complete random design. The subjects were 25 male Wistar rats aged 3 months. Subjects were divided into 5 groups and each group received treatment for 28 days. Data of level HbCO was collected and analyzed by Anova One Way test at 95% confidence level. Results: The results showed a difference between the average levels of HbCO male Wistar rats (p value=0,000 treated and untreated extracts of melon. Conclusion: Melon extracts effect on decreasing the levels of HbCO due to exposure to cigarette smoke. There were differences in levels of male Wistar rats HbCO on treated and untreated extracts of melon and be given at a dose of 250 IU/day.

  8. HbF增高合并β地中海贫血患者的α-珠蛋白基因拷贝数变异分析%Analysis of copy number variations in α-globin gene in the cases with combined increased levels of HbF with β thalassemia

    Institute of Scientific and Technical Information of China (English)

    刘思平; 宋兰林; 熊丽; 王克; 申洪; 钟梅

    2015-01-01

    目的 阐述α珠蛋白基因拷贝数丢失与增加对胎儿血红蛋白(fetal hemoglobin,HbF)水平的影响.方法 收集15例HbF增高合并β地中海贫血的患者,首先采用Gap-PCR检测常见α-地中海贫血的3种缺失型,之后应用多重探针连接扩增技术对α-珠蛋白基因簇行片段缺失与重复分析.结果 15例患者中,检出-SEA缺失杂合子3例,-α3.7缺失杂合子1例,-α4.2缺失纯合子1例,-α3.7与-SEA双重缺失杂合子1例,α珠蛋白基因簇大片段重复1例,类α-珠蛋白基因杂合缺失1例,7例样本未见α拷贝数的丢失与增加.结论 α拷贝数的增多会生成过多的α链,加重α与β链的不平衡性,同时过量的α链与γ链组成过多的HbF,导致HbF水平的升高;α拷贝数的减少会修正α与β链比例的失衡,减轻β0/β0或β0/β+的贫血症状,这类病例归为中间型β地中海贫血,一般具有较高的HbF水平.%Objective To detect copy number changes of α-globin gene,and analyze molecular mechanism of the impacts of fetal hemoglobin (HbF) levels for α-globin gene copy numbers loss or increase.Methods A total of 15 cases with combined increased levels of fetal hemoglobin with β thalassemia were collected.Firstly,three common α-thalassemia deletions were validated by Gap-PCR.Secondly,the largest deletions of the ββ-globin gene cluster were detected by multiplex ligation-dependent probe amplification (MLPA).Result Among the 15 cases,there was 1 case with duplication of the α-globin gene cluster,3 cases of SEA heterozygote deletion of the α-globin gene,1 cases of alpha 3.7 deletion heterozygote of the α-globin gene,1 case of alpha 4.2 deletion homozygote of the α-globin gene,1 case of deletion homozygote in the like α-globin gene.A compound heterozygous for SEA and alpha 3.7 of the α-globin gene was also detected.However,7 cases showed no copy numbers loss and increase of the the α-globin gene cluster.Conclusion Additional α-globin gene can produce

  9. Impact of age, BMI and HbA1c levels on the genome-wide DNA methylation and mRNA expression patterns in human adipose tissue and identification of epigenetic biomarkers in blood

    DEFF Research Database (Denmark)

    Rönn, Tina; Volkov, Petr; Gillberg, Linn

    2015-01-01

    Increased age, BMI and HbA1c levels are risk factors for several non-communicable diseases. However, the impact of these factors on the genome-wide DNA methylation pattern in human adipose tissue remains unknown. We analyzed the DNA methylation of ∼480 000 sites in human adipose tissue from 96 ma...... of candidate genes for obesity, type 2 diabetes and cancer in human adipose tissue. Importantly, we demonstrate that epigenetic biomarkers in blood can mirror age-related epigenetic signatures in target tissues for metabolic diseases such as adipose tissue....... males and 94 females and related methylation to age, BMI and HbA1c. We also compared epigenetic signatures in adipose tissue and blood. Age was significantly associated with both altered DNA methylation and expression of 1050 genes (e.g. FHL2, NOX4 and PLG). Interestingly, many reported epigenetic...... to, for example, RAB37, TICAM1 and HLA-DPB1. Pathway analyses demonstrated that methylation levels associated with age and BMI are overrepresented among genes involved in cancer, type 2 diabetes and cardiovascular disease. Our results highlight the impact of age, BMI and HbA1c on epigenetic variation...

  10. Impact of age, BMI and HbA1c levels on the genome-wide DNA methylation and mRNA expression patterns in human adipose tissue and identification of epigenetic biomarkers in blood.

    Science.gov (United States)

    Rönn, Tina; Volkov, Petr; Gillberg, Linn; Kokosar, Milana; Perfilyev, Alexander; Jacobsen, Anna Louisa; Jørgensen, Sine W; Brøns, Charlotte; Jansson, Per-Anders; Eriksson, Karl-Fredrik; Pedersen, Oluf; Hansen, Torben; Groop, Leif; Stener-Victorin, Elisabet; Vaag, Allan; Nilsson, Emma; Ling, Charlotte

    2015-07-01

    Increased age, BMI and HbA1c levels are risk factors for several non-communicable diseases. However, the impact of these factors on the genome-wide DNA methylation pattern in human adipose tissue remains unknown. We analyzed the DNA methylation of ∼480 000 sites in human adipose tissue from 96 males and 94 females and related methylation to age, BMI and HbA1c. We also compared epigenetic signatures in adipose tissue and blood. Age was significantly associated with both altered DNA methylation and expression of 1050 genes (e.g. FHL2, NOX4 and PLG). Interestingly, many reported epigenetic biomarkers of aging in blood, including ELOVL2, FHL2, KLF14 and GLRA1, also showed significant correlations between adipose tissue DNA methylation and age in our study. The most significant association between age and adipose tissue DNA methylation was found upstream of ELOVL2. We identified 2825 genes (e.g. FTO, ITIH5, CCL18, MTCH2, IRS1 and SPP1) where both DNA methylation and expression correlated with BMI. Methylation at previously reported HIF3A sites correlated significantly with BMI in females only. HbA1c (range 28-46 mmol/mol) correlated significantly with the methylation of 711 sites, annotated to, for example, RAB37, TICAM1 and HLA-DPB1. Pathway analyses demonstrated that methylation levels associated with age and BMI are overrepresented among genes involved in cancer, type 2 diabetes and cardiovascular disease. Our results highlight the impact of age, BMI and HbA1c on epigenetic variation of candidate genes for obesity, type 2 diabetes and cancer in human adipose tissue. Importantly, we demonstrate that epigenetic biomarkers in blood can mirror age-related epigenetic signatures in target tissues for metabolic diseases such as adipose tissue.

  11. Yahtzee: An Anonymized Group Level Matching Procedure

    CERN Document Server

    Jones, Jason J; Fariss, Christopher J; Settle, Jaime E; Kramer, Adam; Marlow, Cameron; Fowler, James H

    2011-01-01

    Researchers often face the problem of needing to protect the privacy of subjects while also needing to integrate data that contains personal information from diverse data sources in order to conduct their research. The advent of computational social science and the enormous amount of data about people that is being collected makes protecting the privacy of research subjects evermore important. However, strict privacy procedures can make joining diverse sources of data that contain information about specific individual behaviors difficult. In this paper we present a procedure to keep information about specific individuals from being "leaked" or shared in either direction between two sources of data. To achieve this goal, we randomly assign individuals to anonymous groups before combining the anonymized information between the two sources of data. We refer to this method as the Yahtzee procedure, and show that it performs as expected theoretically when we apply it to data from Facebook and public voter records.

  12. Mechanosensitive promoter region in the human HB-GAM gene

    DEFF Research Database (Denmark)

    Liedert, Astrid; Kassem, Moustapha; Claes, Lutz;

    2009-01-01

    expression through specific transcription factor binding sites in the promoter region of mechanosensitive genes. In the present study, we demonstrate that the expression of HB-GAM, which is known to have stimulating effects on osteogenic differentiation, is rapidly induced by mechanical loading in hMSC-TERT4...... cells. Analysis of the human HB-GAM gene upstream regulatory region with luciferase reporter gene assays revealed that the upregulation of HB-GAM expression occurred at the transcriptional level and was mainly dependent on the HB-GAM promoter region most upstream containing three potential AP-1 binding...

  13. Yahtzee: an anonymized group level matching procedure.

    Directory of Open Access Journals (Sweden)

    Jason J Jones

    Full Text Available Researchers often face the problem of needing to protect the privacy of subjects while also needing to integrate data that contains personal information from diverse data sources. The advent of computational social science and the enormous amount of data about people that is being collected makes protecting the privacy of research subjects ever more important. However, strict privacy procedures can hinder the process of joining diverse sources of data that contain information about specific individual behaviors. In this paper we present a procedure to keep information about specific individuals from being "leaked" or shared in either direction between two sources of data without need of a trusted third party. To achieve this goal, we randomly assign individuals to anonymous groups before combining the anonymized information between the two sources of data. We refer to this method as the Yahtzee procedure, and show that it performs as predicted by theoretical analysis when we apply it to data from Facebook and public voter records.

  14. Phenotypic Diversity of Sickle Cell Disease in Patients with a Double Heterozygosity for Hb S and Hb D-Punjab.

    Science.gov (United States)

    Torres, Lidiane S; Okumura, Jéssika V; Belini-Júnior, Édis; Oliveira, Renan G; Nascimento, Patrícia P; Silva, Danilo G H; Lobo, Clarisse L C; Oliani, Sonia M; Bonini-Domingos, Claudia R

    2016-09-01

    Phenotypic heterogeneity for sickle cell disease is associated to several genetic factors such as genotype for sickle cell disease, β-globin gene cluster haplotypes and Hb F levels. The coinheritance of Hb S (HBB: c.20A > T) and Hb D-Punjab (HBB: c.364G > C) results in a double heterozygosity, which constitutes one of the genotypic causes of sickle cell disease. This study aimed to assess the phenotypic diversity of sickle cell disease presented by carriers of the Hb S/Hb D-Punjab genotype and the Bantu [- + - - - -] haplotype. We evaluated medical records from 12 patients with sickle cell disease whose Hb S/Hb D-Punjab genotype and Bantu haplotype were confirmed by molecular analysis. Hb S and Hb D-Punjab levels were quantified by chromatographic analysis. Mean concentrations of Hb S and Hb D-Punjab were 44.8 ± 2.3% and 43.3 ± 1.8%, respectively. Painful crises were present in eight (66.7%) patients evaluated, representing the most common clinical event. Acute chest syndrome (ACS) was the second most prevalent manifestation, occurring in two individuals (16.7%). Three patients were asymptomatic, while another two exhibited greater diversity of severe clinical manifestations. Medical records here analyzed reported a significant clinical diversity in sickle cell disease ranging from the absence of symptoms to wide phenotypic variety. The sickle cell disease genotype, Bantu haplotype and hemoglobin (Hb) levels did not influence the clinical diversity. Thus, we concluded that the phenotypic variation in sickle cell disease was present within a specific genotype for disease regardless of the β-globin gene cluster haplotypes.

  15. Transient neonatal cyanosis associated with a new Hb F variant: Hb F viseu.

    Science.gov (United States)

    Bento, Celeste; Magalhães Maia, Tabita; Carvalhais, Ines; Moita, Filipa; Abreu, Gabriela; Relvas, Luis; Pereira, Alexandra; Farela Neves, José; Ribeiro, Maria L

    2013-03-01

    Neonatal cyanosis in healthy newborns can be associated either with methemoglobin due to cytochrome b5 reductase deficiency or to M-hemoglobin, a group of hemoglobin variants resulting from mutations in the globin chain genes. We report the clinical case of a neonate with cyanosis and normal cardiac and respiratory function. At birth the hematological parameters were normal; however, the methemoglobinemia was 16%. Spontaneously, the cyanosis gradually decreased and by the fifth month of age the methemoglobin level was normal. A heterozygous Gγ-globin gene (HBG2) missense mutation 87 C-A (Leu28Met) was identified. His father, with a history of transfusion in the neonatal period, is heterozygous for the same mutation. This hemoglobin variant, not previously described, was called Hb F Viseu and is the sixth Gγ-chain variant reported in association with neonatal cyanosis.

  16. HIGH MATERNAL HbA1c IS ASSOCIATED WITH NEONATAL HYPOCALCEMIA

    Directory of Open Access Journals (Sweden)

    Abdul Tawab

    2014-10-01

    Full Text Available INTRODUCTION: Hypocalcemia occurs within the first 72 hours of birth in up to 50% of IDMs. The objective of the study was to compare the incidence of hypocalcemia in Infants of Pre-Gestational Diabetic & Gestational Diabetic mothers and to find the association of hypocalcemia and maternal diabetic control. MATERIAL AND METHODS: A tertiary care teaching hospital based prospective study over a period of 2 years. 40 Cases were included in the study 20 breast fed full term infants of Gestational diabetic mothers and 20 breastfed full term infants of Pre-Gestational diabetic mothers were enrolled in the study. Serum calcium of neonates in both groups was sent at 48 hours of life. Mean maternal HbA1c level was used to access maternal diabetic control. OBSERVATION AND RESULTS: Neonates of GDM showed statistically significant change in the serum calcium levels (Mean calcium level 9.055 mg/dl and maternal HBA1c levels (mean HbA1c level 5.85% an compared to neonates of PGDM (Mean calcium level 8.015 mg/dl, mean HbA1c level 6.75% (p=0.0001. 7 out of 20 neonates (35 % of Pre Gestational Diabetic mothers had hypocalcemia at 48 hour postnatal age and 3 out of 20 neonates (15% of Gestational Diabetic mothers had hypocalcemia at 48 hour postnatal age. Incidence of hypocalcemia is more when diabetes is poorly controlled [HbA1C>6%] in both groups and it was statistically significant. CONCLUSION: Infants born to Pre-Gestational diabetic mothers are at a higher risk of developing hypocalcemia than infants of gestational diabetic mothers. Incidence of hypocalcemia can be predicted with diabetic control [maternal HbA1c levels]. Studies with larger sample size will give more definitive results on this issue.

  17. Does Group-Level Commitment Predict Employee Well-Being?

    DEFF Research Database (Denmark)

    Clausen, Thomas; Christensen, Karl Bang; Nielsen, Karina

    2015-01-01

    -level psychological well-being, self-reported sickness absence, and sleep disturbances (T2). The association between group-level AOC (T1) and psychological well-being (T2) was fully mediated by individual-level AOC (T1), and the associations between group-level AOC (T1) and self-reported sickness absence and sleep......OBJECTIVE: To investigate the links between group-level affective organizational commitment (AOC) and individual-level psychological well-being, self-reported sickness absence, and sleep disturbances. METHODS: A total of 5085 care workers from 301 workgroups in the Danish eldercare services...

  18. The Relation of Changes in Prescribing Patterns of Anti-diabetic Drugs and HbA1c Levels Among Patients with Type 2 Diabetes Mellitus: Systematic Analysis of Studies From the the Past 20 Years in Turkey

    Directory of Open Access Journals (Sweden)

    Hasan İlkova

    2011-12-01

    Full Text Available Background: The level of glycemic control is considered to be too low among patients with type 2 diabetes mellitus (DM in Turkey. Despite availability of several anti-diabetic treatment alternatives, substantial amount of diabetic patients are at increased risk of diabetes-related macrovascular as well as microvascular complications.Objective: To evaluate glycemic control, disease-related complications and treatment patterns in patients with type 2 DM in Turkey and the effect of novel anti-diabetic therapeutics, especially, insulin analogues in on these parameters. Material and Method: In this systematic review, we included studies on type 2 diabetes mellitus patients published in national and international journals and the abstracts presented in the congresses held in Turkey from 1990 to 2010. The common property of these publications was the inclusion of study population ofadult type 2 diabetes mellitus patients with available data regarding HbA1c values, frequency of diabetes-related complications, and treatments. Of 136 publications screened, a total of 92 were considered eligible to be analysed and were included in the present systematic review. Results and Conclusion: The mean±SD HbA1c level was determined to be 8.34%±0.90% in studies providing data on HbA1c level (64 publications with 16.378 patients This value was 8.90% in publications of 1990-1999 while decreased to 8.28% in 2000-2008. The rates of patients with appropriate glycemic control (HbA1c level of <7% as recommended in American Diabetes Association (ADA guidelines or ≤6.5% as recommended in American Association of Clinical Endocrinologists (AACE guidelines were 30.1% and 20.7%, respectively. There was a significant increase in the rate of of patients having appropriate glycemic control within the period of 2000-2008 compared with the 1990-1999. In the same period, the regression was also evident for the rate of patients with retinopathy amongst DM related-complications from

  19. The identification of beta-thalassemia mutants in Brazilians with high Hb F levels Identificação de mutantes de beta talassemia em grupo de indivíduos com Hb Fetal aumentada da população brasileira

    Directory of Open Access Journals (Sweden)

    Paula J. A. Zamaro

    2010-01-01

    Full Text Available Hemoglobinopathies are a heterogeneous group of genetic disorders which represent a public health problem, with significant morbidity, in countries where the prevalence is high. This study aimed at identifying molecular abnormalities that might explain the laboratorial profile obtained using electrophoresis and high performance liquid chromatography in a group of individuals without signs or clinical symptoms of anemia. Five different mutations for beta-thalassemia were found using PCR-ASO: three cases with CD 6 (-A, one CD 39, one IVI I-6, one -87 (mutations originating in the Mediterranean region and one IVS II-654 (mutation originating in Asia. This is the first time that the CD 6 (-A, -87 and IVS II-654 mutations have been described in the Brazilian population.As hemoglobinopatias são um grupo de afecções genéticas que representam problema de saúde pública em muitos países em que sua incidência é alta, com significativa morbidade. Objetivamos identificar defeitos moleculares que pudessem explicar o perfil laboratorial obtido por eletroforese e HPLC com Hb F elevada, em um grupo de indivíduos adultos sem sinais ou sintomas de anemia. Encontramos cinco diferentes mutações que originam beta talassemia por PCR-ASO: três casos com CD 6 (-A, um CD 39, um IVS 1-5, um -87 todas de origem mediterrânea, e um IVS II-654 de origem asiática. As mutações CD 6 (-A, -87 e IVS II-654 foram descritas pela primeira vez na população brasileira.

  20. β地中海贫血杂合子基因突变及Gγ珠蛋白基因-158位点SNP与Hb F的关系%β-thalassemia mutations and single nucleotide polymorphism at -158 of Gγ-globin gene associated with altered levels of Hb F in β-thalassemia heterozygotes

    Institute of Scientific and Technical Information of China (English)

    陈剑锋; 龙桂芳; 林伟雄; 陈萍

    2004-01-01

    目的探讨β地中海贫血(简称β地贫)杂合子基因突变类型和Gγ珠蛋白基因启动子-158位点(Gγ-158)单核苷酸多态性与胎儿血红蛋白(fetal hemoglobin, Hb F)水平的关系. 方法抗碱-比色法测定 Hb F水平;PCR-寡核苷酸斑点杂交法检测β地贫基因型;限制性内切酶Xmn Ⅰ消化经PCR扩增的Gγ基因启动子DNA片段,分析Gγ-158位点的单核苷酸多态性. 结果 63例受检的轻型β地贫中15例Hb F≥2%(2.06%~10.44%).共检出6种β地贫基因突变,分别是:CD41/42(-TTCT)、CD17(A→T)、nt-28(A→G)、CD71/72(+A)、IVS-II-654(C→T)、IVS-I-1(G→T).CD41/42、CD17、CD71/72、IVS-II-654的杂合子在15例Hb F升高组和48例Hb F正常组各自所占比例相同.63例个体中有10例为Gγ-158(C→T)突变的杂合子,总检出率为15.9%;其中15例高Hb F个体中检出8例(检出率53.33%), Hb F正常的48例检出2例(检出率4.17%),两组检出率差异有显著性(P<0.001). 结论β地贫基因突变CD41/42、CD17、CD71/72、IVS-II-654与β地贫杂合子的Hb F水平无关;而Gγ-158(C→T)突变与广西地区β地贫杂合子Hb F升高密切相关.

  1. Multiple Group Analysis in Multilevel Structural Equation Model Across Level 1 Groups.

    Science.gov (United States)

    Ryu, Ehri

    2015-01-01

    This article introduces and evaluates a procedure for conducting multiple group analysis in multilevel structural equation model across Level 1 groups (MG1-MSEM; Ryu, 2014). When group membership is at Level 1, multiple group analysis raises two issues that cannot be solved by a simple extension of the standard multiple group analysis in single-level structural equation model. First, the Level 2 data are not independent between Level 1 groups. Second, the standard procedure fails to take into account the dependency between members of different Level 1 groups within the same cluster. The MG1-MSEM approach provides solutions to these problems. In MG1-MSEM, the Level 1 mean structure is necessary to represent the differences between Level 1 groups within clusters. The Level 2 model is the same regardless of Level 1 group membership. A simulation study examined the performance of MUML (Muthén's maximum likelihood) estimation in MG1-MSEM. The MG1-MSEM approach is illustrated for both a multilevel path model and a multilevel factor model using empirical data sets.

  2. Experiencing aggression in clubs: social group and individual level predictors.

    Science.gov (United States)

    Miller, Brenda A; Bourdeau, Beth; Johnson, Mark; Voas, Robert

    2015-05-01

    To examine the social drinking group's influence on the individual's experiences of physical or sexual aggression at clubs, data were collected from 368 groups (N = 986 individuals). Both group and individual level indicators were examined for impact on self-reports of physical and sexual aggression experiences while at the club. Recent aggressive experiences and perpetration, concerns for group safety, one's own plans and assessment of other group members' plans to drink to the point of intoxication, and personal characteristics were examined, using both individual and group indicators. At exit, participants reported experiencing physical aggression (12.3 %) and sexual aggression (12.6 %) at the club. Using generalized linear mixed modeling to account for nested data (club, event, and group), group level indicators predicted both the individual's physical and sexual aggression experiences. Especially for experiences of physical aggression, group effects are notable. Being in a group whose members recently experienced physical aggression increased the risk for the individual. Interestingly, groups that had higher levels of planned intoxication decreased risks of experiencing aggression, while a discrepancy in these intentions among group members increased the risks. Group effects were also noted for experiencing sexual aggression. High levels of prior experiences for sexual aggression in the group increased the risks for the individual during the event. Also, being in a group that is identified as having at least one member who is frequently drunk increases the risk for experiencing sexual aggression. These findings inform prevention strategies for young adults engaged in high-risk behaviors by targeting social drinking groups who frequent clubs.

  3. The cultural evolution of emergent group-level traits.

    Science.gov (United States)

    Smaldino, Paul E

    2014-06-01

    Many of the most important properties of human groups - including properties that may give one group an evolutionary advantage over another - are properly defined only at the level of group organization. Yet at present, most work on the evolution of culture has focused solely on the transmission of individual-level traits. I propose a conceptual extension of the theory of cultural evolution, particularly related to the evolutionary competition between cultural groups. The key concept in this extension is the emergent group-level trait. This type of trait is characterized by the structured organization of differentiated individuals and constitutes a unit of selection that is qualitatively different from selection on groups as defined by traditional multilevel selection (MLS) theory. As a corollary, I argue that the traditional focus on cooperation as the defining feature of human societies has missed an essential feature of cooperative groups. Traditional models of cooperation assume that interacting with one cooperator is equivalent to interacting with any other. However, human groups involve differential roles, meaning that receiving aid from one individual is often preferred to receiving aid from another. In this target article, I discuss the emergence and evolution of group-level traits and the implications for the theory of cultural evolution, including ramifications for the evolution of human cooperation, technology, and cultural institutions, and for the equivalency of multilevel selection and inclusive fitness approaches.

  4. The development of an OxyHb animal model in mice and the study on OxyHb-induced apoptosis of mouse brain cells in vivo

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Objective On the basis of developing a new animal model for oxyhemoglobin(OxyHb)injection into subarachnoid space in mice,this research was to explore the temporal dependence and spatial distribution of OxyHb-induced apoptosis in the mouse brain cells in vivo and the mechanism of neurocyte injury induced by OxyHb.Methods The animal model for OxyHb injection into subarachnoid space in mice was developed.Mice were divided randomly into the experimental group(n=40)and the control group(n=35).The control group ...

  5. [Level at which control objectives are reached in patients in different population groups with type 2 diabetes].

    Science.gov (United States)

    Herrero, A; Pinillos, J; Sabio, P; Martín, J L; Garzón, G; Gil, Á

    2016-11-23

    There is evidence of increased macro- and micro-vascular risk in diabetic patients. The objective of this study was to determine the level of control in patients in different population groups with type 2 diabetes. Descriptive cross-sectional study. Primary care. Madrid Health Service. Year: 2014. Patients over 14 years with type 2 diabetes. Number of patientes: n=6674. Variables on the degree of control (HbA1c, systolic blood pressure [SBP], diastolic blood pressure [DBP], LDL-c) and variables on patient characteristics (demographic, other cardiovascular risk factors, complications). The mean age of patients with controlled HbA1c was 67.8 years vs. 62.9 years in the uncontrolled (P 140mmHg or DBP> 90mmHg. Over 25% of patients with hypertension or DL and uncontrolled levels were not receiving drug treatment. Control was improved in all groups, especially in younger patients, with particularly high cardiovascular risk by the presence of other cardiovascular risk factors or macroangiopathy. A significant percentage of patients with uncontrolled BP and cLDL were not diagnosed or receiving drug treatment. Copyright © 2016 Sociedad Española de Médicos de Atención Primaria (SEMERGEN). Publicado por Elsevier España, S.L.U. All rights reserved.

  6. Factors regulating Hb F synthesis in thalassemic diseases

    Directory of Open Access Journals (Sweden)

    Lerone Maria

    2002-02-01

    Full Text Available Abstract Background The thalassemic syndromes originate from mutations of the globin genes that cause, besides the characteristic clinical picture, also an increased Hb F amount. It is not yet clear if there are more factors, besides the beta globin genotype, determining the Hb F production. We have tried to find out if there are relations between total Hb and Hb F, between erythropoietin (Epo and Hb F, between Hb F and point mutations of the gamma gene promoters. Materials and Methods Hematologic parameters, iron status, alpha/non-alpha globin ratio, Epo level, and thalassemic defects of the alpha-, beta-, and gamma-globin genes were explored using standard methods in patients affected by thalassemic diseases. Ninety-five non thalassemic individuals have been examined as controls. Results Two clinical variants of beta-thalassemia intermedia referred to as beta-thal int sub-silent and evident are associated with distinct sets of mutations of the beta-globin gene. Silent beta thal mutations are invariably associated with sub-silent beta thal int; beta° or severe beta+ thal mutations are associated with evident beta thal int (88% and almost invariably (98% with thalassemia major. A positive correlation was observed between the severity of the disease and the Hb F level, but no correlation was found between the Hb F and erythropoietin (Epo level. The mutation Ggamma -158 C→T was detected in 26.9% of patients affected by beta-thal int sub-silent and evident, respectively, but only in 2% of patients with thalassemia major. Conclusions The severity of beta-thal int and the increased Hb F level are strictly dependent from the type of beta-globin gene mutations. No relation is found between Hb F synthesis and Epo secretion. The mutation Ggamma -158 C→T, common among patients affected by beta-thal int and very rare in thal major patients, does not seem, in this study, to influence the Hb F content in beta thal int patients.

  7. Speaking up in groups: a cross-level study of group voice climate and voice.

    Science.gov (United States)

    Morrison, Elizabeth Wolfe; Wheeler-Smith, Sara L; Kamdar, Dishan

    2011-01-01

    Despite a growing body of research on employee voice—defined as the discretionary communication of ideas, suggestions, or opinions intended to improve organizational or unit functioning—the effects of shared or collective-level cognitions have received scant attention. There has also been relatively little research on voice within work groups. Our goal in this study was to address these important gaps by focusing on the effects of group-level beliefs about voice (i.e., group voice climate) on individual voice behavior within work groups. We conducted a cross-level investigation of voice behavior within 42 groups of engineers from a large chemical company. Consistent with our hypotheses, group voice climate was highly predictive of voice and explained variance beyond the effects of individual-level identification and satisfaction, and procedural justice climate. Also consistent with predictions, the effect of identification on voice was stronger in groups with favorable voice climates. These findings provide evidence that voice is shaped not just by individual attitudes and perceptions of the work context, as past research has shown, but also by group-level beliefs. The results also highlight the importance of broadening our conceptual models of voice to include shared cognitions and of conducting additional cross-level research on voice.

  8. The correlation between the Glycated hemoglobin (HbA1c) in non-diabetics and cardiovascular risk factors.

    Science.gov (United States)

    Wu, Xinling; Zhao, Youmin; Chai, Jianwen; Hao, Dongqin

    2016-01-01

    This study aimed to discuss the relativity between the glycated hemoglobin (HbA1c) in non-diabetics and cardiovascular risk factors and definite the significance of predicting the cardiovascular risk factors through cross-sectional research method. There were 2007 cases volunteers (including 650 cases of male, 1357 cases of female) from city community with complete information involved in the research of diabetes. The value of HbA1c 6.5% was set as the diagnose boundary of the diabetes. Differences were considered to be statistically significant at Prisk factors. Then we analyzed the number of risk factors for individuals in different HbA1c groups. Meanwhile, patients were grouped into zero, one, two, three, four or more groups with reference to the number of risk factors they had in order to compare the values of risk factors in different groups through Logistic regression. The results showed that (1) For those people who had no less than three risk factors, the frequency of risk factors was on the rise with the increase of HbA1c levels. (2) The value of HbA1c in different groups of risk factors rose with the increasing number of risk factors. There was a significant difference (Prisk factors. HbA1c levels, which can be a prediction index for cardiovascular risk factors dependent from other cardiovascular risk factors for non-diabetics, and it were highly relevant with impaired glucose tolerance (IGT) and impaired fasting blood glucose (FBG).

  9. Comparative haematological parameters of HbAA and HbAS genotype children infected with Plasmodium falciparum malaria in Yemen.

    Science.gov (United States)

    Albiti, Anisa H; Nsiah, Kwabena

    2014-04-01

    Sickle haemoglobin (HbS) is known to offer considerable protection against falciparum malaria. However, the mechanism of protection is not yet completely understood. In this study, we investigate how the presence of the sickle cell trait affects the haematological profile of AS persons with malaria, in comparison with similarly infected persons with HbAA. This study is based on the hypothesis that the sickle cell trait plays a protective role against malaria. Children from an endemic malaria transmission area in Yemen were enrolled in this study. Hematological parameters were estimated using manual methods, the percentage of parasite density on stained thin smear was calculated, haemoglobin genotypes were determined on paper electrophoresis, ferritin was measured using enzyme-linked immunosorbent assay, serum iron and TIBC were assayed using spectrophotometer, transferrin saturation index was calculated by dividing serum iron by TIBC and expressing the result as a percentage. Haematological parameters were compared in HbAA- and HbAS-infected children. Falciparum malaria parasitaemia was confirmed in the blood smears of 62 children, 44 (55.7%) of AA and 18 (37.5%) AS, so there was higher prevalence in HbAA children (P = 0.047). Parasite density was lower in HbAS- than HbAA-infected children (P = 0.003). Anaemia was prominent in malaria-infected children, with high proportions of moderate and severe forms in HbAA (P = 0.001). The mean levels of haemoglobin, packed cell volume, reticulocyte count, platelets count, lymphocytes, eosinophils, and serum iron were significantly lower while total leukocytes, immature granulocytes, monocytes, erythrocyte sedimentation rate, transferrin saturation, and serum ferritin were significantly higher in HbAA-infected children than HbAS-infected children. Infection with Plasmodium falciparum malaria caused more significant haematological alterations of HbAA children than HbAS. This study supports the observation that sickle cell trait

  10. A pilot interventional study to evaluate the impact of cholecalciferol treatment on HbA1c in type 1 diabetes (T1D

    Directory of Open Access Journals (Sweden)

    R Perchard

    2017-05-01

    Full Text Available Background: Higher 25(OHD3 levels are associated with lower HbA1c, but there are limited UK interventional trials assessing the effect of cholecalciferol on HbA1c. Aims: (1 To assess the baseline 25(OHD3 status in a Manchester cohort of children with type 1 diabetes (T1D. (2 To determine the effect of cholecalciferol administration on HbA1c. Methods: Children with T1D attending routine clinic appointments over three months in late winter/early spring had blood samples taken with consent. Participants with a 25(OHD3 level 10 years units. HbA1c levels before and after treatment were recorded. Results: Vitamin D levels were obtained from 51 children. 35 were Caucasian, 11 South Asian and 5 from other ethnic groups. 42 were vitamin D deficient, but 2 were excluded from the analysis. All South Asian children were vitamin D deficient, with mean 25(OHD3 of 28 nmol/L. In Caucasians, there was a negative relationship between baseline 25(OHD3 level and HbA1c (r = −0.484, P < 0.01. In treated participants, there was no significant difference in mean HbA1c at 3 months (t = 1.010, P = 0.328 or at 1 year (t = −1.173, P = 0.248 before and after treatment. One-way ANCOVA, controlling for age, gender, ethnicity, BMI and diabetes duration showed no difference in Δ HbA1c level. Conclusion: We report important findings at baseline, but in children treated with a stat dose of cholecalciferol, there was no effect on HbA1c. Further studies with larger sample sizes and using maintenance therapy are required.

  11. Physical Fitness Attributes of Team-Handball Players are Related to Playing Position and Performance Level.

    Science.gov (United States)

    Massuca, Luis; Branco, Braulio; Miarka, Bianca; Fragoso, Isabel

    2015-03-01

    Investigations have reported differences amongst player position groups in elite team-Handball (HB) players. Nevertheless, studies with normative physical fitness data of the HB playing positions at more than two different levels of male HB players have not been reported yet. This study aimed: 1) to describe and compare the physical fitness (PF) attributes of male HB players in different playing positions, and 2) to determine which combination of PF measures best discriminate the performance level groups in each one of the individual HB playing position groups. One hundred and sixty-one male HB players participated in this study. The participants were divided into five playing position groups: 1) Goalkeeper (GK, n = 24), 2) Wing (W, n = 48), 3) Back left/right (BLR, n = 38), 4) Back center (BC, n = 29), 5) Pivot (Pi, n = 22), complementarily, performance level was recorded for each participant according to the national HB association, i.e. 1) Top Elite, 2) Moderate Elite, 3) Sub-Elite or, 4) Moderately Trained. Stature and body mass measures were taken from each HB player, and six fitness tests were performed (30 -m sprint, handgrip, vertical jumps-SJ and CMJ, sit-ups, and Yo-Yo IE2). Significant differences were observed between HB playing position groups in body size, speed, and lower limb power and handgrip strength. Nevertheless, 1) the performance in Yo-Yo IE2 was the best measure to discriminate the performance level groups when considering the HB goalkeeper group, HB center back group, and HB pivot group; 2) the average leg power (in squat jump) and the number of executions in sit up test successfully discriminated HB wing performance level groups; and, 3) Stature, countermovement jump height and the position in the Yo-Yo IE2, successfully discriminated HB left/right back performance level groups. It can be concluded that HB players profile, 1) differs according to HB playing position group, and, 2) for the same playing position group, it differs according

  12. [Relationship of consumption of high glycemic index food in the diet and levels of HbA1c in type 2 diabetic patients treated with diet and/or metformin].

    Science.gov (United States)

    Varela, Nicol; Vega, Claudia; Valenzuela, Karen

    2012-03-01

    At present there is still no clear consensus on recommendations on the use of GI of foods for the dietary management of T2DM. Rather different entities propose the use of carbohydrate counting, because there is not even enough evidence for dietary planning based on this index. The aim of this study was to relate consumption of high GI food with glycemic control of type 2 diabetes patients from the cardiovascular health program of 3 CESFAM (Family Health Centers) in Santiago, Chile. Forty individuals were selected, anthropometric measurements were conducted as well as a modified poll of frequency of food consumption of 30 days. Data from GI, GL, number of servings with high GI consumed per day and total amount of CARB consumed per day. Correlations were determined with values of HbA1c of the last 3 month obtained from the medical record. The average age was 58.6 +/- 9.5 years. The percentage of obesity was 62.5% and the average BMI was 32.5. The average HbA1c value was 7.08 +/- 1.6, for HbA1c HbA1c and number of servings with high GI (r = 0.56 p = 0.002). For the remaining variables there was no statistically significant correlation (p > 0.05). For each extra serving of high GI food there was an increase of 0.9% of HbA1c. In our research population the amount of food with high GI ingested per day was significantly correlated with values of HbA1c.

  13. HbA1C and cancer risk in patients with type 2 diabetes--a nationwide population-based prospective cohort study in Sweden.

    Directory of Open Access Journals (Sweden)

    Junmei Miao Jonasson

    Full Text Available BACKGROUND: Diabetes is associated with increased cancer risk. The underlying mechanisms remain unclear. Hyperglycemia might be one risk factor. HbA1c is an indicator of the blood glucose level over the latest 1 to 3 months. This study aimed to investigate association between HbA1c level and cancer risks in patients with type 2 diabetes based on real life situations. METHODS: This is a cohort study on 25,476 patients with type 2 diabetes registered in the Swedish National Diabetes Register from 1997-1999 and followed until 2009. Follow-up for cancer was accomplished through register linkage. We calculated incidences of and hazard ratios (HR for cancer in groups categorized by HbA1c ≤ 58 mmol/mol (7.5% versus >58 mmol/mol, by quartiles of HbA1c, and by HbA1c continuously at Cox regression, with covariance adjustment for age, sex, diabetes duration, smoking and insulin treatment, or adjusting with a propensity score. RESULTS: Comparing HbA1c >58 mmol/mol with ≤ 58 mmol/mol, adjusted HR for all cancer was 1.02 [95% CI 0.95-1.10] using baseline HbA1c, and 1.04 [95% CI 0.97-1.12] using updated mean HbA1c, and HRs were all non-significant for specific cancers of gastrointestinal, kidney and urinary organs, respiratory organs, female genital organs, breast or prostate. Similarly, no increased risks of all cancer or the specific types of cancer were found with higher quartiles of baseline or updated mean HbA1c, compared to the lowest quartile. HR for all cancer was 1.01 [0.98-1.04] per 1%-unit increase in HbA1c used as a continuous variable, with non-significant HRs also for the specific types of cancer per unit increase in HbA1c. CONCLUSIONS: In this study there were no associations between HbA1c and risks for all cancers or specific types of cancer in patients with type 2 diabetes.

  14. REDUCTION OF CLASSROOM NOISE LEVELS USING GROUP CONTINGENCIES

    OpenAIRE

    Ring, Brandon M; Sigurdsson, Sigurdur O.; Eubanks, Sean L.; Silverman, Kenneth

    2014-01-01

    The therapeutic workplace is an employment-based abstinence reinforcement intervention for unemployed drug users where trainees receive on-the-job employment skills training in a classroom setting. The study is an extension of prior therapeutic workplace research, which suggested that trainees frequently violated noise standards. Participants received real-time graphed feedback of noise levels and had the opportunity to earn monetary group reinforcement for maintaining a low number of noise v...

  15. Group-level component analyses of EEG: validation and evaluation

    Directory of Open Access Journals (Sweden)

    Rene eHuster

    2015-07-01

    Full Text Available Multi-subject or group-level component analysis provides a data-driven approach to study properties of brain networks. Algorithms for group-level data decomposition of functional magnetic resonance imaging data have been brought forward more than a decade ago and have significantly matured since. Similar applications for electroencephalographic data are at a comparatively early stage of development though, and their sensitivity to topographic variability of the electroencephalogram or loose time-locking of neuronal responses has not yet been assessed. This study investigates the performance of independent component analysis (ICA and second order blind source identification (SOBI for data decomposition, and their combination with either temporal or spatial concatenation of data sets, for multi-subject analyses of electroencephalographic data. indent Analyses of simulated sources with different spatial, frequency, and time-locking profiles, revealed that temporal concatenation of data sets with either ICA or SOBI served well to reconstruct sources with both strict and loose time-locking, whereas performance decreased in the presence of topographical variability. The opposite pattern was found with a spatial concatenation of subject-specific data sets.This study proofs that procedures for group-level decomposition of electroencephalographic data can be considered valid and promising approaches to infer the latent structure of multi-subject data sets. Yet, specific implementations need further adaptations to optimally address sources of inter-subject and inter-trial variance commonly found in EEG recordings.

  16. Commentary: improving persistently elevated HbA1c in diabetes mellitus patients in Nigeria.

    Science.gov (United States)

    Oghagbon, Efosa K

    2014-01-01

    Glycated hemoglobin (HbA1c) level in patients with diabetes reflects quality of disease control and propensity to develop hyperglycemic complications. During more than 12 years of using HbA1c for monitoring of glycemic control among patients at Nigerian hospitals, the mean glycated hemoglobin ranged from 7.9% ± 2.4 to 8.3% ± 2.2. Most of these patients (63% to 68%) had poor glycemic controls with mean HbA1c greater than 7%. Factors that are implicated in this scenario are: 1) high cost of HbA1c testing, 2) ineffective management of risk factors, 3) poor patient compliance, 4) improperly managed diabetes education program, and 5) health care system defect. Central to improving diabetes glycemia is education of doctors, other health workers and patients, within the confines of an overhauled national health system. Physicians need to increase adherence to diabetes mellitus management guidelines and patients must be enrolled into a well-structured education program at health centers. Doctors, as leader of the health team, should drive such education schemes, which must be based on standard training curriculum, sufficient number of trained diabetes educators, and effective monitoring of patients. The most appropriate diabetes education model features small-to-moderate sized participant groups and makes use of motivational interviewing rather than a traditional advice-giving format. Improved health care funding is mandatory given the issue of cost and this can be helped by increased participation of patients in Nigeria's National Health Insurance Scheme. Failure to address the persistently elevated HbA1c will affect long-term quality of life, longevity and health care services in Nigeria.

  17. Relationship between HbA1c levels and risk of cardiovascular adverse outcomes and all-cause mortality in overweight and obese cardiovascular high-risk women and men with type 2 diabetes

    DEFF Research Database (Denmark)

    Andersson, C; van Gaal, L; Caterson, I D

    2012-01-01

    The optimal HbA(1c) concentration for prevention of macrovascular complications and deaths in obese cardiovascular high-risk patients with type 2 diabetes remains to be established and was therefore studied in this post hoc analysis of the Sibutramine Cardiovascular OUTcomes (SCOUT) trial, which...... enrolled overweight and obese patients with type 2 diabetes and/or cardiovascular disease....

  18. Node Grouping in System-Level Fault Diagnosis

    Institute of Scientific and Technical Information of China (English)

    ZHANG Dafang; XIE Gaogang; MIN Yinghua

    2001-01-01

    With the popularization of network applications and multiprocessor systems,dependability of systems has drawn considerable attention. This paper presents a new technique of node grouping for system-level fault diagnosis to simplify the complexity of large system diagnosis. The technique transforms a complicated system to a group network, where each group may consist of many nodes that are either fault-free or faulty. It is proven that the transformation leads to a unique group network to ease system diagnosis. Then it studies systematically one-step t-faults diagnosis problem based on node grouping by means of the concept of independent point sets and gives a simple sufficient and necessary condition. The paper presents a diagnosis procedure for t-diagnosable systems. Furthermore, an efficient probabilistic diagnosis algorithm for practical applications is proposed based on the belief that most of the nodes in a system are fault-free. The result of software simulation shows that the probabilistic diagnosis provides high probability of correct diagnosis and low diagnosis cost, and is suitable for systems of any kind of topology.

  19. Hb mass measurement suitable to screen for illicit autologous blood transfusions.

    Science.gov (United States)

    Pottgiesser, Torben; Umhau, Markus; Ahlgrim, Christoph; Ruthardt, Sebastian; Roecker, Kai; Schumacher, Yorck Olaf

    2007-10-01

    An increase of hemoglobin (Hb) mass is the key target of blood doping practices to enhance performance as it is a main determinant of maximal oxygen uptake. Although detection methods exist for doping with recombinant EPO and homologous blood transfusions, autologous transfusions remain virtually undetectable. In this context, the most sensitive parameter would be a determination of Hb mass itself. The purpose therefore was to establish whether Hb mass measurements by the optimized CO-rebreathing method allow screening for the withdrawal and reinfusion of autologous red blood cells. The optimized CO-rebreathing method was used for evaluation of Hb mass in two groups at three time points (duplicate measurements: 1) baseline, 2) after donation, and 3) after reinfusion). Group I (N = 6) was to donate and receive 1 unit of packed red cells (PRC) in contrast to two PRC in group II (N = 4). The time span between withdrawal and reinfusion was 2 d. The mean Hb content of the blood units was 59.0 +/- 3.9 g (group I) and 108.3 +/- 1.3 g (group II). Hb mass decreased significantly after blood withdrawal (-89 +/- 16 g in group I and -120 +/- 14 g in group II) and increased significantly after reinfusion (group I: 70 +/- 16 g; group II: 90 +/- 9 g) but was lower than at baseline (group I: -19 +/- 17 g; group II: -30 +/- 14 g). The total error of measurements for the duplicate measures ranged between 0.8 and 3.1% (Hb mass: 6.4-22.1 g). Hb mass determination with the optimized CO-rebreathing method has sufficient precision to detect the absolute differences in Hb mass induced by blood withdrawal and autologous reinfusion. Thus, it may be suited to screen for artificially induced alterations in Hb mass.

  20. Discrete time duration models with group-level heterogeneity

    DEFF Research Database (Denmark)

    Frederiksen, Anders; Honoré, Bo; Hu, Loujia

    2007-01-01

    Dynamic discrete choice panel data models have received a great deal of attention. In those models, the dynamics is usually handled by including the lagged outcome as an explanatory variable. In this paper we consider an alternative model in which the dynamics is handled by using the duration...... in the current state as a covariate. We propose estimators that allow for group-specific effect in parametric and semiparametric versions of the model. The proposed method is illustrated by an empirical analysis of job durations allowing for firm-level effects....

  1. Role of Altered Venous Blood Lactate and HbA1c in Women with Gestational Diabetes Mellitus

    Science.gov (United States)

    Santhosh, N U; Krishnamurthy, N; Chethan, Chethana; Shilpashree, M K

    2016-01-01

    Introduction Being a mirror image of metabolic syndrome, Gestational Diabetes Mellitus (GDM) is associated with significant maternal and fetal morbidity. Increased blood lactate concentration and alterations of substrate utilization are partly involved in development of insulin resistance in GDM. Fetuses born to such mothers have shown low umbilical vein oxygen saturation and low oxygen content and increased lactate concentrations. These changes may certainly reflect enhanced fetal metabolism as a result of hyperglycaemia and hyperinsulinemia and therefore, these fetuses deserve intense surveillance at term and during delivery. Ideally, HbA1c should be maintained below 5% during their first trimesters and below 6% during third trimester. We planned to investigate GDM women for their HbA1c levels too. Aim To know if there is any alteration in blood lactate and/or HbA1c levels and to know if there is any correlation between these two parameters in GDM pregnancies, in comparison with the previous studies which measured lactate in cord blood and placental vessels of GDM women. Materials and Methods It was a hospital based prospective study on 40 women with gestational diabetes and 40 age-matched normal pregnant women. We analysed the biochemical and metabolic mileau in these women by estimating venous blood lactate and HbA1c levels. We paid special attention to follow them up regarding maternal complications if any and perinatal outcomes. The independent samples t-test and Pearson’s correlation test were applied. Results GDM mothers showed significantly higher lactate and HbA1c levels than normal pregnant women, both with p<0.001. Blood pressure and fetal birth weight were also significantly higher in GDM group than Normal Pregnant (NP) group, both with p-values of <0.001. Further, this increased lactate levels showed significant positive correlation with HbA1c, blood pressure and fetal birth weight. Conclusion Maternal blood lactate and HbA1c levels have a

  2. Thermo-mechanical model optimization of HB-LED packaging

    NARCIS (Netherlands)

    Yuan, C.A.; Erinc, M.; Gielen, A.W.J.; Waal, A. van der; Driel, W. van; Zhang, K.

    2011-01-01

    Lighting is an advancing phenomenon both on the technology and on the market level due to the rapid development of the solid state lighting technology. The efforts in improving the efficacy of high brightness LED's (HB-LED) have concentrated on the packaging architecture. Packaging plays a significa

  3. Patients newly diagnosed with clinical type 2 diabetes mellitus but presenting with HbA1c within normal range: 19-year mortality and clinical outcomes

    DEFF Research Database (Denmark)

    Veloso, A.G.; Siersma, V.; Heldgaard, P.E.;

    2013-01-01

    AIMS: To investigate whether long-term mortality or clinical outcomes differed between patients diagnosed with type 2 diabetes mellitus and presenting with HbA1c within or above normal range at time of diagnosis. METHODS: Data were from a population-based sample of 1136 individuals with newly...... diagnosed type 2 diabetes mellitus. The diagnosis was confirmed with a single fasting whole blood/plasma glucose ≥7.0/8.0mmol/l. The median time from day of diagnosis until end of follow up was 18.8years. Patients were grouped according to normal HbA1c and elevated HbA1c at diagnosis. The effect of elevated...... HbA1c on a number of clinical outcomes and all-cause mortality was assessed in Cox regression models. RESULTS: At diagnosis, 97 patients (8.5%) had an HbA1c level within normal range. Age (mean (SD)) at diagnosis was 64.5 (11.5) years. Both unadjusted and adjusted hazard ratios for the effect of HbA1...

  4. Trophic groups and modules: two levels of group detection in food webs.

    Science.gov (United States)

    Gauzens, Benoit; Thébault, Elisa; Lacroix, Gérard; Legendre, Stéphane

    2015-05-06

    Within food webs, species can be partitioned into groups according to various criteria. Two notions have received particular attention: trophic groups (TGs), which have been used for decades in the ecological literature, and more recently, modules. The relationship between these two group concepts remains unknown in empirical food webs. While recent developments in network theory have led to efficient methods for detecting modules in food webs, the determination of TGs (groups of species that are functionally similar) is largely based on subjective expert knowledge. We develop a novel algorithm for TG detection. We apply this method to empirical food webs and show that aggregation into TGs allows for the simplification of food webs while preserving their information content. Furthermore, we reveal a two-level hierarchical structure where modules partition food webs into large bottom-top trophic pathways, whereas TGs further partition these pathways into groups of species with similar trophic connections. This provides new perspectives for the study of dynamical and functional consequences of food-web structure, bridging topological and dynamical analysis. TGs have a clear ecological meaning and are found to provide a trade-off between network complexity and information loss.

  5. Stability study for magnetic reagent assaying Hb and HbA1c

    Energy Technology Data Exchange (ETDEWEB)

    Hsieh, Wen-Pin [Actherm Inc., Hsinchu 200, Taiwan (China); Chieh, J.J.; Yang, C.C. [Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan (China); Yang, S.Y. [Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan (China); MagQu Co., Ltd., Sindian Dist., New Taipei City 231, Taiwan (China); Chen, Po-Yu; Huang, Yu-Hao [Actherm Inc., Hsinchu 200, Taiwan (China); Hong, Y.W. [Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan (China); Horng, H.E., E-mail: phyfv001@ntnu.edu.tw [Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan (China)

    2013-01-15

    Reagents for magnetically labeled immunoassay on human Hb and human HbA1c have been synthesized. The reagents consist of Fe{sub 3}O{sub 4} magnetic particles biofunctionalized with antibodies against Hb and HbA1c. It has been demonstrated that the reagents can be applied to quantitatively detect Hb and HbA1c by using immunomagnetic reduction assay. In addition to characterizing the assay properties, such as the standard curve and the low-detection limit, the stability of reagents is investigated. To do this, the temporal dependence of particle sizes and the bio-activity of reagents are monitored. The results show that the reagents are highly stable when stored at 2-8 Degree-Sign C. This means that the reagents synthesized in this work are promising for practical applications. - Highlights: Black-Right-Pointing-Pointer The properties of assaying Hb and HbA1c using immunomagnetic reduction are studied. Black-Right-Pointing-Pointer The magnetic nanoparticles with antibodies are highly stable in solutions. Black-Right-Pointing-Pointer No significant mutual interference between Hb and HbA1c in assays is observed. Black-Right-Pointing-Pointer High-sensitivity assays on Hb and HbA1c using immunomagnetic reduction are achieved.

  6. Using HB Family of Protocols for Privacy-Preserving Authentication of RFID Tags in a Population

    CERN Document Server

    Halevi, Tzipora; Halevi, Shai

    2009-01-01

    In this paper, we propose an HB-like protocol for privacy-preserving authentication of RFID tags, whereby a tag can remain anonymous and untraceable to an adversary during the authentication process. Previous proposals of such protocols were based on PRF computations. Our protocol can instead be used on low-cost tags that may be incapable of computing standard PRFs. Moreover, since the underlying computations in HB protocols are very efficient, our protocol also reduces reader load compared to PRF-based protocols. We suggest a tree-based approach that replaces the PRF-based authentication from prior work with a procedure such as HB+ or HB#. We optimize the tree- traversal stage through usage of a "light version" of the underlying protocol and shared random challenges across all levels of the tree. This provides significant reduction of the communication resources, resulting in a privacy-preserving protocol almost as efficient as the underlying HB+ or HB#

  7. Design Features of the Diabetes and Periodontal Therapy Trial (DPTT): A Multicenter Randomized Single-Masked Clinical Trial Testing the Effect of Non-surgical Periodontal Therapy on Glycosylated Hemoglobin (HbA1c) Levels in Subjects with Type 2 Diabetes and Chronic Periodontitis

    Science.gov (United States)

    2013-01-01

    Background Evidence suggests that periodontitis is associated with prevalent and incident type 2 diabetes mellitus (T2DM), raising the question of whether periodontitis treatment may improve glycemic control in patients with T2DM. Meta-analyses of mostly small clinical trials suggest that periodontitis treatment results in a modest reduction in glycosylated hemoglobin (Hb) A1c. Purpose The purpose of the Diabetes and Periodontal Therapy Trial (DPTT) was to determine if periodontal treatment reduces HbA1c in patients with T2DM and periodontitis. Methods DPTT was a phase-III, single-masked, multi-center, randomized trial with a planned enrollment of 600 participants. Participants were randomly assigned to receive periodontal treatment immediately (Treatment Group) or after 6 months (Control Group). HbA1c values and clinical periodontal measures were determined at baseline and 3 and 6 months following randomization. Medication usage and dosing were assessed at each visit. Periodontal treatment consisted of scaling and root planing for a minimum of two 90-minute sessions, plus the use of an antibacterial mouth rinse for at least 32 days afterwards. The primary outcome was change in HbA1c from baseline to 6 months and the trial was powered to detect a between-group difference of 0.6%. Secondary outcomes included changes in periodontal clinical measures, fasting plasma glucose, the Homeostasis Model Assessment (HOMA2) and the need for rescue diabetes or periodontal therapy. Conclusion Dental and medical researchers collaborated to recruit, treat and monitor participants with two chronic diseases to determine if treatment of one condition affects the status of the other. PMID:24080100

  8. Design features of the Diabetes and Periodontal Therapy Trial (DPTT): a multicenter randomized single-masked clinical trial testing the effect of nonsurgical periodontal therapy on glycosylated hemoglobin (HbA1c) levels in subjects with type 2 diabetes and chronic periodontitis.

    Science.gov (United States)

    Engebretson, S; Gelato, M; Hyman, L; Michalowicz, B S; Schoenfeld, E

    2013-11-01

    Evidence suggests that periodontitis is associated with prevalent and incident type 2 diabetes mellitus (T2DM), raising the question of whether periodontitis treatment may improve glycemic control in patients with T2DM. Meta-analyses of mostly small clinical trials suggest that periodontitis treatment results in a modest reduction in glycosylated hemoglobin (Hb) A1c. The purpose of the Diabetes and Periodontal Therapy Trial (DPTT) was to determine if periodontal treatment reduces HbA1c in patients with T2DM and periodontitis. DPTT was a phase-III, single-masked, multi-center, randomized trial with a planned enrollment of 600 participants. Participants were randomly assigned to receive periodontal treatment immediately (Treatment Group) or after 6 months (Control Group). HbA1c values and clinical periodontal measures were determined at baseline and 3 and 6 months following randomization. Medication usage and dosing were assessed at each visit. Periodontal treatment consisted of scaling and root planing for a minimum of two 90-minute sessions, plus the use of an antibacterial mouth rinse for at least 32 days afterwards. The primary outcome was change in HbA1c from baseline to 6 months and the trial was powered to detect a between-group difference of 0.6%. Secondary outcomes included changes in periodontal clinical measures, fasting plasma glucose, the Homeostasis Model Assessment (HOMA2) and the need for rescue diabetes or periodontal therapy. Dental and medical researchers collaborated to recruit, treat and monitor participants with two chronic diseases to determine if treatment of one condition affects the status of the other. © 2013. Published by Elsevier Inc. All rights reserved.

  9. The discussion of HbA1C in differentiation of stress hyperglycemia and diabetes hyperglycemia in craniocerebral trauma patients%HbA1c在鉴别颅脑外伤患者应激性血糖升高与糖尿病血糖升高的探讨

    Institute of Scientific and Technical Information of China (English)

    石建设; 李上华; 何彪

    2014-01-01

    Objective:To investigate the glycosylated hemoglobin (HbA1c) increased value and diabetes blood sugar rise high in the differential diagnosis of craniocerebral trauma patients with stress hyperglycemia.Methods:Retrospective analysis of our hospital in 2012 May -2013 year in April 210 cases with craniocerebral trauma hospitalized and hyperglycemia in patients with clinical data, al patients were glycosylated hemoglobin, and selected healthy persons as control group of 60 cases, two patients were given corresponding treatment, to observe the changes of blood glucose and glycosylated hemoglobin in the course of treatment and the control of blood glucose and HbA1c level changes in patients with fasting group.Results:According to the glycosylated hemoglobin levels wil be divided into two groups, HbA1c group (117 cases of low stress hyperglycemia) and high HbA1c group (93 cases of diabetic hyperglycemia). Compared with the control group, two groups were HbA1c content increased, the content of HbA1c and diabetic hyperglycemia group was significantly higher than that of stress hyperglycemia group, there are significant differences in contrast.Conclusions:HbA1c is a good index for identification of patients with craniocerebral trauma stress hyperglycemia and diabetic hyperglycemia in patients after craniocerebral injury, should put the glycosylated hemoglobin detection as a packet according to the treatment plan, to take timely and proper treatment.%目的:探讨糖化血红蛋白(HbA1c)在鉴别颅脑外伤患者应激性血糖升高与糖尿病血糖升高中的应用价值。方法:回顾性分析我院于2012年5月-2013年4月收治的210例因颅脑外伤住院且血糖升高患者的临床资料,所有患者均进行糖化血红蛋白检测,同时选取健康体检人员60例作为对照组,前两组患者均给予相应的治疗,观察治疗过程中血糖和糖化血红蛋白的变化规律以及对照组患者的空腹血糖和HbA1c水平变化规

  10. Novel permeability characteristics of red blood cells from sickle cell patients heterozygous for HbS and HbC (HbSC genotype).

    Science.gov (United States)

    Dalibalta, S; Ellory, J C; Browning, J A; Wilkins, R J; Rees, D C; Gibson, J S

    2010-06-15

    Individuals heterozygous for HbS and HbC (HbSC) represent about 1/3(rd) of sickle cell disease (SCD) patients. Whilst HbSC disease is generally milder, there is considerable overlap in symptoms with HbSS disease. HbSC patients, as well as HbSS ones, present with the chronic anaemia and panoply of acute vaso-occlusive complications that characterize SCD. However, there are important clinical and haematological differences. Certain complications occur with greater frequency in HbSC patients (like proliferative retinopathy and osteonecrosis) whilst intravascular haemolysis is reduced. Patients with HbSC disease can be considered as a discrete subset of SCD cases. Although much work has been carried out on understanding the pathogenesis of SCD in HbSS homozygotes, including the contribution of altered red blood cell permeability, relatively little pertains directly to HbSC individuals. Results reported in the literature suggest that HbSC cells, and particularly certain subpopulations, present with similar permeability to HbSS cells but there are also important differences - these have not been well characterized. We hypothesise that their unique cell transport properties accounts for the different pattern of disease in HbSC patients and represents a potential chemotherapeutic target not shared in red blood cells from HbSS patients. The distinct pattern of clinical haematology in HbSC disease is emphasised here. We analyse some of the electrophysiological properties of single red blood cells from HbSC patients, comparing them with those from HbSS patients and normal HbAA individuals. We also use the isosmotic haemolysis technique to investigate the behaviour of total red blood cell populations. Whilst both HbSS and HbSC cells show increased monovalent and divalent (Ca(2+)) cation conductance further elevated upon deoxygenation, the distribution of current magnitudes differs, and outward rectification is greatest for HbSC cells. In addition, although Gd(3+) largely

  11. Norms as Group-Level Constructs: Investigating School-Level Teen Pregnancy Norms and Behaviors.

    Science.gov (United States)

    Mollborn, Stefanie; Domingue, Benjamin W; Boardman, Jason D

    2014-09-01

    Social norms are a group-level phenomenon, but past quantitative research has rarely measured them in the aggregate or considered their group-level properties. We used the school-based design of the National Longitudinal Study of Adolescent Health to measure normative climates regarding teen pregnancy across 75 U.S. high schools. We distinguished between the strength of a school's norm against teen pregnancy and the consensus around that norm. School-level norm strength and dissensus were strongly (r = -0.65) and moderately (r = 0.34) associated with pregnancy prevalence within schools, respectively. Normative climate partially accounted for observed racial differences in school pregnancy prevalence, but norms were a stronger predictor than racial composition. As hypothesized, schools with both a stronger average norm against teen pregnancy and greater consensus around the norm had the lowest pregnancy prevalence. Results highlight the importance of group-level normative processes and of considering the local school environment when designing policies to reduce teen pregnancy.

  12. The group exponential lasso for bi-level variable selection.

    Science.gov (United States)

    Breheny, Patrick

    2015-09-01

    In many applications, covariates possess a grouping structure that can be incorporated into the analysis to select important groups as well as important members of those groups. One important example arises in genetic association studies, where genes may have several variants capable of contributing to disease. An ideal penalized regression approach would select variables by balancing both the direct evidence of a feature's importance as well as the indirect evidence offered by the grouping structure. This work proposes a new approach we call the group exponential lasso (GEL) which features a decay parameter controlling the degree to which feature selection is coupled together within groups. We demonstrate that the GEL has a number of statistical and computational advantages over previously proposed group penalties such as the group lasso, group bridge, and composite MCP. Finally, we apply these methods to the problem of detecting rare variants in a genetic association study.

  13. HbA1c Test in Identification between Stress Hyperglycemia and Diabetic Hyperglycemia%探究HbA1c对应激性与糖尿病性高血糖的鉴别作用

    Institute of Scientific and Technical Information of China (English)

    冯秋花; 钱家乐

    2013-01-01

    目的 探讨应激状态下糖化血红蛋白(HbA1c)测定是否能鉴别应激性高血糖与糖尿病性高血糖.方法 选择2009年1月-2012年1月收治的有应激因素参与的高血糖患者100例,将其作为临床研究对象.对100例患者进行HbA1c水平检测,2~3周后,所有患者待应激因素消除后,再做口服葡萄糖耐量试验(OGTT),若未达糖耐量异常(IGT)或糖尿病(DM)诊断标准(其中DM的诊断标准采用1999年WHO国际通用标准),则为应激性高血糖.确诊患者中应激性高血糖患者及糖尿病高血糖患者人数,将1例新发糖尿病、2例应激性高血糖患者归为糖尿病组.将再选取体检人员60例作为对照组,分别对糖尿病组高血糖患者、应激组高血糖(SH)患者与对照组进行空腹血糖及HbA1c水平检测.观察并分析三组患者空腹血糖及HbA1c水平.结果 HbA1c诊断的结果与OGTT检测结果接近,在判断糖尿病高血糖和应激性高血糖两者之间的准确性高.糖尿病组高血糖患者和应激组高血糖患者的空腹血糖浓度和HbA1c水平均较对照组提高,糖尿病高血糖患者的空腹血糖浓度和HbA1c水平较应激组患者高(P<0.05).结论 HbA1c是鉴别应激性高血糖和糖尿病的一个良好指标,可应用于临床鉴别SH与真性DM,值得在临床上广泛推广.%Objective To discuss whether HbAlc test can identify stress hyperglycemia and diabetic hyperglycemia in the stress state. Methods A total of 100 patients with stress hyperglycemia were enrolled in this study from January 2009 to January 2012. Their HbAlc level was tested. OGTT was performed when the stress factors had been eliminated 2 to 3 weeks late. The patients who did not meet the diagnostic criteria of TGT or DM were diagnosed as stress hyperglycemia (SH). One case of new-onset diabetes patients and two cases of IGT patients were chosen as diabetes group,60 cases of physical examination staff were as control group. The fasting blood

  14. Challenges in HbA1c Analysis and Reporting in Patients with Variant Hemoglobins.

    Science.gov (United States)

    Sultana, T A; Sheme, Z A; Sultana, G S; Sultana, B; Mishu, F A; Khan, N Z; Sarkar, B C; Muttalib, M A; Khan, S A; Choudhury, S; Mahtab, H

    2016-04-01

    Hemoglobin A1c (HbA(1)c) is a well-established indicator of mean glycemia. The presence of genetic variants of hemoglobin can profoundly affect the accuracy of HbA(1)c measurements. Variants of hemoglobin especially Hemoglobin E (HbE) is prevalent in South East Asia including Bangladesh. The objective of our study is to compare the HbA(1)c values measured on high performance liquid chromatography (HPLC) and Turbidimetric Inhibition Immunoassay (TINIA) in diabetic patients with variant hemoglobins including HbE. A total of 7595 diabetic patients receiving treatment at BIRDEM General Hospital were analyzed for HbA(1)c results within a period of two months from December 2013 to January 2014. Seventy two cases out of 7595 (0.95%) had either undetectable or below normal HbA(1)c levels (males-33 and females-39; ratio = 0.82:1) by HPLC method. In 34(0.45%) cases, HbA(1)c value was undetectable by HPLC method but was in the reportable range by TINIA method. In the other 38 (0.55%) cases, HbA(1)c levels were below the reportable range (Hb variant, proper knowledge of hemoglobin variants which affect the measurements HbA(1)c level is essential. MCV of 80fl or below may serve as a rough guide to select samples that require analysis by TINIA method. Moreover, HPLC may be a convenient and inexpensive tool for screening of hemoglobinopathies especially among diabetic population in Bangladesh. It may therefore be helpful in improving management of complications related to both anaemia and iron overload.

  15. Diagnostic value analysis of glycated hemoglobin A1c(HbA1c) for high blood pressure diagnosis%糖化血红蛋白A1c(HbA1c)对高血压的诊断价值分析

    Institute of Scientific and Technical Information of China (English)

    苗伟

    2016-01-01

    Objective: To analyzed the diagnostic value of glycated hemoglobin A1c (HbA1c) for high blood pressure. Methods: Selected 60 cases of hypertension patients in our hospital in September 2014 - September 2015 were analyzed retrospectively, and set them to the reaserch group, the other 48 cases selected physical examination as a control group without high blood pressure, observe and compare the diagnostic value of two groups of HbA1c application.Results:The reaserch group of HbA1c and 1 h, 2 h postprandial, 3 HPG level were higher than the control group (P<0.05).Conclusions:HbA1c has high diagnostic value, with HbA1c determination may disorder of glucose metabolism in patients with high blood pressure be found early, and with high blood pressure could be a reference as the main symptoms of diabetes diagnosis.%目的:分析高血压患者中应用糖化血红蛋白A1c(HbA1c)的诊断价值。方法:资料选取本院2014年9月-2015年9月收治的60例高血压患者进行回顾性分析,将其设为研究组,另选取48例无高血压的健康体检者作为对照组,观察与比较两组应用HbA1c的诊断价值。结果:研究组HbA1c与餐后1h、2h、3hPG水平均比对照组高(P<0.05)。结论:HbA1c诊断价值高,经HbA1c测定可以对高血压患者糖代谢的紊乱情况予以早期发现,并对以高血压作为主要症状糖尿病诊断具有借鉴作用。

  16. Application of CRISPRi for prokaryotic metabolic engineering involving multiple genes, a case study: Controllable P(3HB-co-4HB) biosynthesis.

    Science.gov (United States)

    Lv, Li; Ren, Yi-Lin; Chen, Jin-Chun; Wu, Qiong; Chen, Guo-Qiang

    2015-05-01

    Clustered regularly interspaced short palindromic repeats interference (CRISPRi) is used to edit eukaryotic genomes. Here, we show that CRISPRi can also be used for fine-tuning prokaryotic gene expression while simultaneously regulating multiple essential gene expression with less labor and time consumption. As a case study, CRISPRi was used to control polyhydroxyalkanoate (PHA) biosynthesis pathway flux and to adjust PHA composition. A pathway was constructed in Escherichia coli for the production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] from glucose. The native gene sad encoding E. coli succinate semi-aldehyde dehydrogenase was expressed under the control of CRISPRi using five specially designed single guide RNAs (sgRNAs) for regulating carbon flux to 4-hydroxybutyrate (4HB) biosynthesis. The system allowed formation of P(3HB-co-4HB) consisting of 1-9mol% 4HB. Additionally, succinate, generated by succinyl-coA synthetase and succinate dehydrogenase (respectively encoded by genes sucC, sucD and sdhA, sdhB) was channeled preferentially to the 4HB precursor by using selected sgRNAs such as sucC2, sucD2, sdhB2 and sdhA1 via CRISPRi. The resulting 4HB content in P(3HB-co-4HB) was found to range from 1.4 to 18.4mol% depending on the expression levels of down-regulated genes. The results show that CRISPRi is a feasible method to simultaneously manipulate multiple genes in E. coli.

  17. HB+ inserted into the CMS Solenoid

    CERN Multimedia

    Tejinder S. Virdee, CERN

    2006-01-01

    The first half of the barrel hadron calorimeter (HB+) has been inserted into the superconducting solenoid of CMS, in preparation for the magnet test and cosmic challenge. The operation went smoothly, lasting a couple of days.

  18. Toxicity of cypermethrin to Daphnia magna HB

    Institute of Scientific and Technical Information of China (English)

    SHENG Xiu-mei; XIONG Li; WU Zhen-bin; TANG Hong-feng; LIU Tao; WANG Yuan

    2004-01-01

    The acute toxic effect of the pesticide cypermethrin to Daphnia magna HB was examined. D. magna HB was exposed to cypermethrin at concentrations of 0, 1, 3, 5, 7, and 9 mg/L for 24 h. Data showed that the 24 h-LC50 of cypermthrin on D. magna HB was 4.81 mg/L. In contrast, the 24 h-LC50 of K2Cr2O7(the national standard toxicant) to Daphnia magna was 0.38 mg/L in the current study. Results indicated that the Daphnia magna was very sensitive to pesticides. In addition, the effects of the culture condition(such as hardness, temperature and DO etc.) on Daphnia magna HB was also studied.

  19. Can HB vaccine yield a booster effect on individuals with positive serum anti-HBs and anti-HBc markers?

    Institute of Scientific and Technical Information of China (English)

    Ru-Xiang Wang; Christina Y. Shi; Ying Guo; Chang-Hong Yang; Yu Song; Juan Chen; Fu-Sheng Pang; Shao-Ping Lei; Xiao-Ming Jia; Jin-Ying Wen

    2004-01-01

    AIM: To evaluate if HB vaccination can yield a booster effecton the anti-HBs level of those naturally acquired HBV positivemarkers.METHODS: Sera were collected from 1399 newly enrolleduniversity students aged between 18-20 years at theentrance medical examination in 2001. Forty-four students(28 males and 16 females) with positive serum anti-HBs and anti-HBc markers served as an observation group and another 44 students (24 males and 20 females) without any HBV markers as the control. HB vaccination was given to all the students without positive serum HBs Ag according to 0,1, 6 month regimen and the peripheral venous blood was sampled from those of both observation and control groups for anti-HBs detection one month after the second and third doses. Anti-HBs levels were measured by ELISA.RESULTS: The seroconversion rate of anti-HBs in the control group was 100% after the second dose, but the geometric mean titers (GMTs) were low. The tendency of serum anti-HBs changes after the 3rd dose was completely different between the two groups. Although more than half of those with positive anti-HBs and anti-HBc showed a mild increase of anti-HBs levels after the 2nd boosting dose (mean anti-HBs level was 320:198 mIU), but the increase of serum anti-HBs titer was much smaller than that in the control group. The averages of their initial serum anti-HBs levelsand the levels after the 2nd and 3rd doses were 198, 320 and 275 mIU respectively. All the subjects from the control group had an obvious increase in their serum anti-HBs levels which was nearly 4 times the baseline level (302:78 mIU).CONCLUSION: HB vaccination can not enhance anti-HBs levels in those with positive serum anti-HBs and anti-HBc markers.

  20. A new sickle cell disease phenotype associating Hb S trait, severe pyruvate kinase deficiency (PK Conakry), and an alpha2 globin gene variant (Hb Conakry).

    Science.gov (United States)

    Cohen-Solal, M; Préhu, C; Wajcman, H; Poyart, C; Bardakdjian-Michau, J; Kister, J; Promé, D; Valentin, C; Bachir, D; Galactéros, F

    1998-12-01

    A Guinean woman, heterozygous for haemoglobin (Hb) S, was studied because of episodes of marked anaemia, repeated typical metaphyseal painful crises and haemosiderosis. Her sickling syndrome resulted from the association of Hb S trait with a severe pyruvate kinase deficiency leading to a 2,3-DPG concentration of twice normal levels. Sequence of the PK-R gene revealed an undescribed mutation in the homozygous or hemizygous state within exon 5 (nucleotide 2670 C-->A), leading to the interchange of Ser 130 into Tyr (PK Conakry). In addition, the patient carried a new haemoglobin variant, Hb Conakry [alpha80(F1) Leu-->Val], which seemed to have a mild effect. The high intraerythrocytic 2,3-DPG concentration induced by the PK deficiency resulted in a decreased oxygen affinity which favoured sickling to a level almost similar to that of Hb S/C compound heterozygous patients. This was confirmed by oxygen binding measurements of Hb A/Hb S erythrocytes in which 2,3-DPG content was modified in vitro. Hysteresis between deoxy- and reoxygenation curves, as well as increase in the n(max) value, demonstrated that the extent of HbS polymerization in the propositus was almost the same as that of RBCs from a homozygous sickle cell patient or those of an A/S heterozygous patient with an artificial in vitro increase of 2,3-DPG concentration.

  1. Interference of hemoglobin E on measurements of glycosylated hemoglobin (HbA1c)%Hb E变异体对5种糖化血红蛋白检测系统测定结果的干扰评价

    Institute of Scientific and Technical Information of China (English)

    温冬梅; 张秀明; 张德才; 庞嘉琳; 吴剑杨; 李曼; 徐全中; 索明环; 萧金丽

    2014-01-01

    Objective To evaluate the interference of hemoglobin variant E (HbE) on five kinds of glycosylated hemoglobin (HbA1c) system.Two of them were ion-exchange high performance liquid chromatography (IE-HPLC) methods,one was affinity chromatography high performance liquid chromatography (AC-HPLC) method,one was turbidimetric inhibition immunoassay method (TINIA),one was enzyme (EM) method.Methods All 60 blood samples from May 2012 to October 2013 were collected from Zhongshan Hospital of Yat-sen University,and then divided into normal control group (20 cases),diabetic group (20 cases) and HbE group (20 cases).Variants were used to detect the whole blood concentration of HbA1c by five detection systems.Based on the judgment standards of National Glycohemoglobin Standardization Program (NGSP),comparison analysis and bias evaluation results for different groups of 5 kinds of detection systems were estimated.The statistical difference of mean blood glucose (eAG) and fasting blood glucose (FPG) was calculated using the estimation of HbE variant group detection system HbA1c results.The test results were analyzed by Deming regression analysis,to determine whether HbE has significant clinical effect on the results of HbA1c,using 6% HbA1c and 9% ± 10% relative error as the evaluation scope.Results The 95% confidence interval (95% CI) of the 4 kinds of detection system in normal control group and diabetic group were within ± 0.7% HbA1c deviation,which was less than 6%,comparing to NGSP Ⅰ laboratory certification,indicating no significant difference of determination results(P > 0.05).There were no significant difference between eAG and FPG levels in HbE group using AC-HPLC,Variant HbE variant Ⅱ IE-HPLC method and TINIA method(P >0.05).While the difference between eAG level and FPG level of Variant Ⅱ Turbo IE-HPLC and enzymatic method was statistically significant (P < 0.01).Bio-rad Variant Ⅱ IE-HPLC method,TINIA method and AC-HPLC method for the

  2. Stability study for magnetic reagent assaying Hb and HbA1c

    Science.gov (United States)

    Hsieh, Wen-Pin; Chieh, J. J.; Yang, C. C.; Yang, S. Y.; Chen, Po-Yu; Huang, Yu-Hao; Hong, Y. W.; Horng, H. E.

    2013-01-01

    Reagents for magnetically labeled immunoassay on human Hb and human HbA1c have been synthesized. The reagents consist of Fe3O4 magnetic particles biofunctionalized with antibodies against Hb and HbA1c. It has been demonstrated that the reagents can be applied to quantitatively detect Hb and HbA1c by using immunomagnetic reduction assay. In addition to characterizing the assay properties, such as the standard curve and the low-detection limit, the stability of reagents is investigated. To do this, the temporal dependence of particle sizes and the bio-activity of reagents are monitored. The results show that the reagents are highly stable when stored at 2-8 °C. This means that the reagents synthesized in this work are promising for practical applications.

  3. Glutathione Redox System in β-Thalassemia/Hb E Patients

    Directory of Open Access Journals (Sweden)

    Ruchaneekorn W. Kalpravidh

    2013-01-01

    Full Text Available β-thalassemia/Hb E is known to cause oxidative stress induced by iron overload. The glutathione system is the major endogenous antioxidant that protects animal cells from oxidative damage. This study aimed to determine the effect of disease state and splenectomy on redox status expressed by whole blood glutathione (GSH/glutathione disulfide (GSSG and also to evaluate glutathione-related responses to oxidation in β-thalassemia/Hb E patients. Twenty-seven normal subjects and 25 β-thalassemia/Hb E patients were recruited and blood was collected. The GSH/GSSG ratio, activities of glutathione-related enzymes, hematological parameters, and serum ferritin levels were determined in individuals. Patients had high iron-induced oxidative stress, shown as significantly increased serum ferritin, a decreased GSH/GSSG ratio, and increased activities of glutathione-related enzymes. Splenectomy increased serum ferritin levels and decreased GSH levels concomitant with unchanged glutathione-related enzyme activities. The redox ratio had a positive correlation with hemoglobin levels and negative correlation with levels of serum ferritin. The glutathione system may be the body’s first-line defense used against oxidative stress and to maintain redox homeostasis in thalassemic patients based on the significant correlations between the GSH/GSSH ratio and degree of anemia or body iron stores.

  4. Converter structure-based power loss and static thermal modeling of the press-pack IGBT-based three-level ANPC and HB VSCs applied to Multi-MW wind turbines

    DEFF Research Database (Denmark)

    Senturk, Osman Selcuk; Munk-Nielsen, Stig; Teodorescu, Remus

    2010-01-01

    and the switch thermal performance which is determined by the converter load profile and the converter structure. In this study, the converter-structure based power loss and thermal models are developed for the medium voltage full-scale 3LANPC- VSC and 3L-HB-VSC utilizing press-pack IGBT-diode pairs....... For the experimental model verifications, a test setup with a single-phase full-scale 3L-ANPC-VSC is introduced. Using the power loss and thermal models, the switch junction temperatures are obtained on simulation for the wind turbine grid interface. The power density and reliability of the VSCs are discussed......The wind turbine converters demand high power density due to nacelle space limitation and high reliability due to high maintenance cost. Once the converter topology with the semiconductor switch technology is selected, the converter power density and reliability are dependent on the component count...

  5. The development of an OxyHb animal model in mice and the study on OxyHb-induced apoptosis of mouse brain cells in vivo

    Institute of Scientific and Technical Information of China (English)

    Shi Wei; Wang Ruizhi; Huang Liyong; Sun Jianjun; Wang Fangru; Liu Chongxiao; Zhou Le; Guo Zhenyu; John H Zhang

    2008-01-01

    Objective On the basis of developing a new animal model for oxyhemoglobin (OxyHb) injection into subarachnoid space in mice, this research was to explore the temporal dependence and spatial distribution of OxyHb- induced apoptosis in the mouse brain cells in vivo and the mechanism of neurocyte injury induced by OxyHb. Methods The animal model for OxyHb injection into subarachnoid space in mice was developed. Mice were divided randomly into the experimental group (n=40) and the control group (n= 35). The control group received saline injection (50 μL ) and the experimental group received OxyHb injection (50 μL ), both into the subarachnoid space. The mice of the two groups were subdivided according to different postoperative time (3 h, 6 h, 12 h, 24 h and 48 h). The apoptosis or necrosis of cells was distinguished with microscopy (HE staining), transmission electron microscopy and TUNEL method. Results The distribution of apoptosis was mainly in the ipsilateral neocortex and bilateral hippocampal gyrus. The apoptotic mouse brain cells showed morphological changes in the experimental group by HE staining and transmission electron microscopy. The count of TUNEL-positive cells showed substantial increase in the experimental group, and there was a significant difference between the control and experimental groups, and the number of OxyHb- induced apoptotic cells decreased with time. Conclusion OxyHb in subarachnoid space in mice can induce apoptasis, but not necrosis of mouse brain cells in viro. The apoptotic brain cells show the pattern of temporal dependence and spatial distribution. It is suggested that the early treatment should be the method of first choice for treating the hemorrhagic brain injury.

  6. Application Experience of U-mAlb Joint HbA1c Test in the Diagnosis of Diabetic Nephropathy%U-mAlb联合HbA1c检测在糖尿病肾病诊断中的应用体会

    Institute of Scientific and Technical Information of China (English)

    王建霞

    2015-01-01

    Objective Analysis of the value of the application of U-mAlb joint HbA1c test in the diagnosis of diabetic nephropathy.MethodsRandomly selected 60 cases of diabetic nephropathy and 58 cases of healthy volunteers for the study,comparative study to explore two objects’U-mAlb,HbA1c concentration differences.ResultsThe U-mAlb,HbA1c levels were(52.9±13.2)mg/L,(7.5±1.2)%. Control group of U-mAlb,HbA1c levels were(9.1±0.3)mg/L,(4.2±1.1)%.Conclusion This study demonstrated that U-mAlb and HbA1c levels and the degree of impairment of renal patients with early diabetes are closely related,for patients with diabetic nephropathy early diagnosis,prevention and treatment has far-reaching significance.%目的:分析研究U-mAlb联合HbA1c检测在糖尿病肾病诊断中的应用价值。方法随机选择60例糖尿病肾病患者和58例健康志愿者作为研究对象,对比探究两组研究对象的U-mAlb、HbA1c浓度差异。结果实验组U-mAlb、HbA1c水平分别为(52.9±13.2)mg/L、(7.5±1.2)%;对照组U-mAlb、HbA1c水平分别为(9.1±0.3)mg/L、(4.2±1.1)%。结论本研究证实U-mAlb和HbA1c水平与糖尿病患者早期肾脏的受损程度有密切的关系,对于糖尿病肾病患者早期诊断、预防和治疗具有作用。

  7. Significance of HbA1c targets based on an individual approach to the treatment of patients with type 2 diabetes mellitus

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    Inna Vladimirovna Misnikova

    2014-05-01

    Full Text Available BackgroundOver the past few years, special attention has been paid to achieving glycaemic control for type 2 diabetes mellitus (T2DM patients, since it is a factor for determining the risk of developing macro- and microvascular complications of diabetes. Certain modern guidelines suggest an individual approach to the choice of HbA1c target.ObjectiveObjective of this study was to estimate the percentage of T2DM patients who have reached the HbA1c levels. This was determined based on their age and the presence of severe complications.Materials and MethodsA total of 2195 patients with T2DM were studied. The patients were divided into the following age groups: <45, 45–64, and over 65 years. Each group was subdivided into two subgroups depending on the presence of severe complications. The target level of HbA1c was determined according to the subjects’ age and the presence of severe complications:<45 years old without complications – HbA1c<6.5%;<45 years old with complications and 45–64 years old without complications – HbA1c <7.0%;45–64 years old with complications and over 65 years old without complications – HbA1c <7.5%;over 65 years old with complications – HbA1c <8%.Statistical analyses were performed using Microsoft Excel. The data are presented as mean values ± standard deviation.ResultsThe following glucose-lowering therapy techniques were used for different groups: monotherapy with diet – 301 (13.7% patients; oral antidiabetic drugs (OADs – 1335 (60.8% patients; combined treatment using OADs with insulin – 319 (14.6% patients; and insulin monotherapy – 240 (10.9% patients. The HbA1c target was reached for 27.3% of patients in the group aged <45 years old without complications; in the group < 45 years old with complications for 25.0% of patients; in the group of 45–64 years old without complications for 30.0% of patients; in the group aged 45–64 with complications for 35.2% of patients; in the group

  8. HB D Los Angeles in a Brazilian family Hb D Los Angeles em família brasileira

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    Guilherme G. Leoneli

    2001-09-01

    Full Text Available Inherited disorders of hemoglobin, the most common monogenic disease, are now well understood at the molecular level, knowledge, which has led to considerable improvements in their control and management. The Brazilian population is multiethnic, and the correct characterization of the Hb D is important, mainly because the method available for detection of abnormal hemoglobins, present a migration in the same zone at alkaline pH, for Hb S, D, and G for example. In this paper we studied a family with an abnormal hemoglobin like S in alkaline electrophoresis, by appropriated methods including HPLC and molecular analysis, characterized as hemoglobin D Los Angeles.As doenças hereditária da hemoglobina são as mais comuns doenças monogênicas e atualmente bem conhecidas do ponto de vista molecular, fato este que propiciou um avanço no seu controle e manuseio. A população brasileira caracteriza-se pela multiplicidade étnica e a caracterização da Hb D torna-se importante por este dado, associado ao fato de que os métodos de detecção das hemoglobinopatias comumente não identificam esta fração anormal que apresenta a peculiaridade de migração eletroforéticia em pH alcalino na mesma zona observada nas Hb S e G. Neste relato é apresentado um estudo familiar no qual é empregada metodologia adequada, o HLPC, que permite a identificação da Hb D.

  9. 低氧训练对大鼠血液内促红细胞生成素、血红蛋白和红细胞水平的影响%Effects of Hypoxic Training on Blood EPO, Hb and RBC Level in Rats

    Institute of Scientific and Technical Information of China (English)

    娄淑杰; 焦颖

    2011-01-01

    Objective To investigate effects of 7 day hypoxic training on the level of Erythropoietin (EPO), hemoglobin(Hb) and red blood cell (RBC) in blood of SD rats, and to obtain the experimental evidence of adaptation to hypoxic training in rats. Methods The rats were exposed in different concentration of hypoxic environment for 2h/day, the time of treadmill running of rats is 30min/day. The level of EPO was checked by ELISA, and the level of RBC and Hb were measured by automatic blood cell analyzer. Results (1) No effects of hypoxic exposure in 15% O2 and 11% O2 environment on EPO level were found significantly, but treadmill running in 15% O2 environment enhance EPO level in blood of rats. (2) The number of RBC was increased significantly in rats which exercised on treadmill or stayed in 15% O2or 11% O2 environment. (3) The increase of concentration of Hb was found significantly in rats which stayed in 15% O2or 11% O2 environment. (4) Effects of hypoxic training on RBC and Hb level disappear in rats 15days after hypoxic training. Conclusion Production of RBC and Hb are signifi cantly induced by hypoxic training in rats, but lasting time of enhancing effect is shorter than 15 days.%目的 观察为期7d的低氧训练对大鼠血液内促红细胞生成素(Erythropoietin,EPO)水平、血红蛋白(Hemoglobin,Hb)水平和红细胞(Red blood cell,RBC)数量的影响,以获得大鼠对低氧训练产生适应的实验证据及为建立适宜的低氧训练模式提供思路.方法 将动物置于不同低氧浓度环境2 h/d,大鼠跑台运动时间为30min/d.使用ELISA方法检测大鼠血清中EPO含量;采用全血自动分析仪检测血液中RBC数量和Hb水平.结果 (1)在11%和15%氧浓度下暴露2 h/d,大鼠血液内EPO未产生明显变化,但在15%氧浓度下暴露的同时进行运动,可明显提高大鼠血液内EPO含量;(2)无论是在11%和15%氧浓度下暴露还是在11%和15%氧浓度下运动,均可明显提高大鼠血液内RBC

  10. University-Level Group Piano Instruction and Professional Musicians

    Science.gov (United States)

    Young, Margaret Mary

    2013-01-01

    Group piano courses for undergraduate music majors have been charged with developing the piano skills used by professional musicians; however, the only information available on the use of piano skills by professional musicians has been limited to one profession: public school music teachers. No one has attempted to identify the piano skills used…

  11. University-Level Group Piano Instruction and Professional Musicians

    Science.gov (United States)

    Young, Margaret Mary

    2013-01-01

    Group piano courses for undergraduate music majors have been charged with developing the piano skills used by professional musicians; however, the only information available on the use of piano skills by professional musicians has been limited to one profession: public school music teachers. No one has attempted to identify the piano skills used…

  12. Group-Level Selection Increases Cooperation in the Public Goods Game.

    Science.gov (United States)

    Eckel, Catherine C; Fatas, Enrique; Godoy, Sara; Wilson, Rick K

    2016-01-01

    When groups compete for resources, some groups will be more successful than others, forcing out less successful groups. Group-level selection is the most extreme form of group competition, where the weaker group ceases to exist, becoming extinct. We implement group-level selection in a controlled laboratory experiment in order to study its impact on human cooperation. The experiment uses variations on the standard linear public goods game. Group-level selection operates through competition for survival: the least successful, lowest-earning groups become extinct, in the sense that they no longer are able to play the game. Additional control treatments include group comparison without extinction, and extinction of the least successful individuals across groups. We find that group-level extinction produces very high contributions to the provision of the public good, while group comparison alone or individual extinction fail to cause higher contributions. Our results provide stark evidence that group-level selection enhances within-group cooperation.

  13. Masters Level Graduate Student Writing Groups: Exploring Academic Identity

    Science.gov (United States)

    Ruggles, Tosha M.

    2012-01-01

    This action research project explores masters level graduate student writing and academic identity during one semester in an interdisciplinary masters program. Informing this study is a two part theoretical framework including the Academic Literacy Model (Lea and Street) and Wenger's concept of identity. The purpose of this exploration was to…

  14. Fortuitous description of hemoglobin Hope in a high-level Tunisian athlete: molecular diagnosis and origin.

    Science.gov (United States)

    Bibi, Amina; Touhemi, Imed; Sahli, Chaima; Siala, Hajer; Bartagi, Zakia; Koubaa, Donia; Le Gallais, Daniel; Fattoum, Slaheddine; Messaoud, Taieb

    2012-01-01

    In this study we report the fortuitous description of hemoglobin (Hb) Hope in a Tunisian athlete. This Hb is one of hemoglobin variants that show a lower stability and oxygen affinity that is beneficial to tissue oxygen delivery. Hb Hope was isolated by automated high performance liquid chromatography and was unequivocally found to be Hb Hope using DNA-based methods: polymerase chain reaction, denaturing gradient gel electrophoresis, direct DNA sequencing. Restriction haplotype showed that this Hb was supported by the Mediterranean haplotype I. Hb Hope was identified at first in a black African-American family and later in several other black and non black ethnic groups. All these descriptions raise the question of the Hb Hope origin. Recently, Hb Hope was reported in Thai in association with the same Mediterranean haplotype I. This favors that Tunisian and Thai Hb Hope would share a common Mediterranean origin, thus suggesting the possibility of a Mediterranean gene flow. On another hand, the observation of Hb Hope in a high level athlete would suggest a selection pressure of this Hb variant due to higher physical aptitude.

  15. Diagnosis of a rare double heterozygous Hb D Punjab/Hb Q India hemoglobinopathy using Sebia capillary zone electrophoresis

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    Sushama Parab

    2014-01-01

    Full Text Available In India, hemoglobinopathies constitute a major genetic disorder and hemoglobin variants such as Hb S, Hb D Punjab, and Hb E are the most common ones. Other variants include Hb Q India, Hb Lepore, Hb J Meerut, Hb D Iran, etc. These variants show heterozygous state along with beta thalassemia. However, compound heterozygosities among these variants are very rare. Ethylenediaminetetraacetic acid whole blood sample received for routine thalassemia screening was subjected to alkaline electrophoresis using automated capillary zone electrophoresis. Suspecting the presence of rare variants, further analysis was carried out using Bio-Rad D10 and Tosoh G8 high-performance liquid chromatography (HPLC systems. Capillary zone electrophoretograms showed the presence of peaks in zone Hb A, Hb D, a fused peak in Hb A2, and a small peak in Z1 zone. Bio-Rad and Tosoh chromatograms also indicated the presence of four peaks which are identified as Hb A, Hb D Punjab, Hb Q India, and hybrid of Hb D Punjab/Hb Q India. A peak in Hb D zone of capillary was due to co-migration of Hb D Punjab and Hb Q India variants. Small peak in Z1 zone indicated the presence of alpha chain variant Hb Q India. The findings were further confirmed by HPLC results and molecular genetic studies. The present study reports for the 1 st time a rare hemoglobinopathy of double heterozygosity for Hb D Punjab, Hb Q India on Capillarys 2 Flex Piercing analyzer and is forth reported case for this rare hemoglobinopathy.

  16. Differential regulation of plasma proteins between members of a family with homozygous HbE and HbEβ-thalassemia

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    Suchismita Halder

    2014-09-01

    Full Text Available In this report we’ve compared the plasma protein profiles of 4 individuals in a family. Father and the younger son both are hemoglobin (Hb Eβ-thalassemic {Cod 26 (G-A/IVS 1- 5 (G-C}, but the father never requires transfusion, whereas the younger son requires monthly blood transfusion. Mother and the elder son are HbEE {Cod 26 (G-A/Cod 26 (GA} without any history of transfusion. Proteomic study was done on the plasma fraction of the blood following ammonium sulphate precipitation. Proteins were separated by 2D-gel electrophoresis, expression of proteins compared by densitometry and proteins identified by tandem MALDI mass spectrometry. Proteins responsible in hemolysis, hypercoagulation and hemoglobin scavenging have shown differential regulation, establishing the relation between the differences in the levels of plasma proteins with the progression of the disease phenotype, manifested in the extent of transfusion dependence of the patient.

  17. Group level effects of social versus individual learning.

    Science.gov (United States)

    Jost, Jürgen; Li, Wei

    2013-06-01

    We study the effects of learning by imitating others within the framework of an iterated game in which the members of two complementary populations interact via random pairing at each round. This allows us to compare both the fitness of different strategies within a population and the performance of populations in which members have access to different types of strategies. Previous studies reveal some emergent dynamics at the population level, when players learn individually. We here investigate a different mechanism in which players can choose between two different learning strategies, individual or social. Imitating behavior can spread within a mixed population, with the frequency of imitators varying over generation time. When compared to a pure population with solely individual learners, a mixed population with both individual and social learners can do better, independently of the precise learning scheme employed. We can then search for the best imitating strategy. Imitating the neighbor with the highest payoff turns out to be consistently superior. This is in agreement with findings in experimental and model studies that have been carried out in different settings.

  18. Transformation with TT8 and HB12 RNAi Constructs in Model Forage (Medicago sativa, Alfalfa) Affects Carbohydrate Structure and Metabolic Characteristics in Ruminant Livestock Systems.

    Science.gov (United States)

    Li, Xinxin; Zhang, Yonggen; Hannoufa, Abdelali; Yu, Peiqiang

    2015-11-04

    Lignin, a phenylpropanoid polymer present in secondary cell walls, has a negative impact on feed digestibility. TT8 and HB12 genes were shown to have low expression levels in low-lignin tissues of alfalfa, but to date, there has been no study on the effect of down-regulation of these two genes in alfalfa on nutrient chemical profiles and availability in ruminant livestock systems. The objectives of this study were to investigate the effect of transformation of alfalfa with TT8 and HB12 RNAi constructs on carbohydrate (CHO) structure and CHO nutritive value in ruminant livestock systems. The results showed that transformation with TT8 and HB12 RNAi constructs reduced rumen, rapidly degraded CHO fractions (RDCA4, P = 0.06; RDCB1, P < 0.01) and totally degraded CHO fraction (TRDCHO, P = 0.08). Both HB12 and TT8 populations had significantly higher in vitro digestibility of neutral detergent fiber (NDF) at 30 h of incubation (ivNDF30) compared to the control (P < 0.01). The TT8 populations had highest ivDM30 and ivNDF240. Transformation of alfalfa with TT8 and HB12 RNAi constructs induced molecular structure changes. Different CHO functional groups had different sensitivities and different responses to the transformation. The CHO molecular structure changes induced by the transformation were associated with predicted CHO availability. Compared with HB12 RNAi, transformation with TT8 RNAi could improve forage quality by increasing the availability of both NDF and DM. Further study is needed on the relationship between the transformation-induced structure changes at a molecular level and nutrient utilization in ruminant livestock systems when lignification is much higher.

  19. Survey and analysis of correlation of HbA1c with other parameters in 4386 employees

    Institute of Scientific and Technical Information of China (English)

    胡守岳

    2012-01-01

    Objective To understand the HbA1c level and analyze its relationship with human physiological indexes. Methods The HbA1c level was measured in enterprise employees aged 45 years or more,using Japanese Tosoh HLC-723 G7 automatic glycohemoglobin analyzer,and the FPG,TC,TG,hsC-RP

  20. Chromatographic analysis of Hb S for the diagnosis of various sickle cell disorders in Pakistan.

    Science.gov (United States)

    Hashmi, Nazish Khalid; Moiz, Bushra; Nusrat, Maliha; Hashmi, Mashhooda Rasool

    2008-08-01

    Sickle cell disease remains a relatively obscure theme in research on haemoglobinopathies in Pakistan. Limited data is available regarding its prevalence in the country. The objective of our study was not only to estimate the frequency of different sickle cell diseases but also to provide quantitative estimation of haemoglobin S and other haemoglobin variants using an automated high-performance liquid chromatography (HPLC) system. For this purpose, we retrospectively evaluated the results of HPLC performed on all patients with suspected haemoglobinopathies during the years 2005 and 2006. Information derived from various sources was used to identify a particular genotype by analysing each sample containing Hb S with respect to haemoglobin, red cell indices and levels of various associated haemoglobin variants. Analysis of 15,699 samples identified 302 patients with Hb S (1.92%). The genotypes identified included Sbeta(0) (46.7%), SS (19.2%), SA (11.6%), Sbeta(+) (8.6%) and SD (2.3%). Thirty-five cases could not be categorised and were labelled 'unclassified'. Majority of the patients (62.3%) were below the age of 18 years. Balochistan, which is the largest province based on the area, yielded the highest number of patients (n = 140). In the Sbeta(0) group, the mean haemoglobin and Hb S were lower in children compared to adults (p value of 0.001 and 0.016, respectively). We conclude that sickle cell disorders are prevalent in Pakistan to a significant extent, being concentrated in certain areas of the country. We present the first report of various haemoglobin S genotypes from our population. It is hoped that it will act as a database to characterise the same for our population.

  1. Avaliação de Hb A2 e Hb F em doadores de sangue de região malarígena da Amazônia Oriental brasileira por HPLC Evaluation of Hb A2 and Hb F by HPLC in blood donors from the malaria endemic region of Eastern Amazon of Brazil

    Directory of Open Access Journals (Sweden)

    Wanessa C. Souza

    2003-01-01

    Full Text Available In malaria endemic regions of Africa, resistance to infection by Plasmodium has been observed in under 6-month-old children, when there are higher fetal hemoglobin (Hb F levels. Research performed in the São José do Rio Preto region, central-east Brazil, reported increased levels of Hb F in blood donors. The purpose of this work was to evaluate the A2 hemoglobin (Hb A2 and Hb F concentrations in blood donors deriving from the Brazilian malaria endemic region. Forty-five blood donor samples from Macapá, from patients with varying genders, ages and ethnic origins, were collected by venous puncture after informed consent was obtained. The samples were analyzed by High Performance Liquid Chromatography (HPLC - System Variant (Bio-Rad. The HPLC demonstrated sensitivity and rapidity in the identification and measurement of the hemoglobins and gave precise results. Moreover, it provided measurement of hemoglobin variants, even when they were present in small amounts, providing a diagnosis of hemoglobinopathies. Hb F levels above the normal were observed in 33.3% of the analyzed samples. The presence of increased Hb F can suggest resistance to infection by Plasmodium falciparum, as there have been reports that infected red blood cells interfere in the development of the parasite.

  2. The Properties of Red Blood Cells from Patients Heterozygous for HbS and HbC (HbSC Genotype

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    A. Hannemann

    2011-01-01

    Full Text Available Sickle cell disease (SCD is one of the commonest severe inherited disorders, but specific treatments are lacking and the pathophysiology remains unclear. Affected individuals account for well over 250,000 births yearly, mostly in the Tropics, the USA, and the Caribbean, also in Northern Europe as well. Incidence in the UK amounts to around 12–15,000 individuals and is increasing, with approximately 300 SCD babies born each year as well as with arrival of new immigrants. About two thirds of SCD patients are homozygous HbSS individuals. Patients heterozygous for HbS and HbC (HbSC constitute about a third of SCD cases, making this the second most common form of SCD, with approximately 80,000 births per year worldwide. Disease in these patients shows differences from that in homozygous HbSS individuals. Their red blood cells (RBCs, containing approximately equal amounts of HbS and HbC, are also likely to show differences in properties which may contribute to disease outcome. Nevertheless, little is known about the behaviour of RBCs from HbSC heterozygotes. This paper reviews what is known about SCD in HbSC individuals and will compare the properties of their RBCs with those from homozygous HbSS patients. Important areas of similarity and potential differences will be emphasised.

  3. Cation Homeostasis in Red Cells From Patients With Sickle Cell Disease Heterologous for HbS and HbC (HbSC Genotype).

    Science.gov (United States)

    Hannemann, A; Rees, D C; Tewari, S; Gibson, J S

    2015-11-01

    Sickle cell disease (SCD) in patients of HbSC genotype is considered similar, albeit milder, to that in homozygous HbSS individuals--but with little justification. In SCD, elevated red cell cation permeability is critical as increased solute loss causes dehydration and encourages sickling. Recently, we showed that the KCl cotransporter (KCC) activity in red cells from HbSC patients correlated significantly with disease severity, but that in HbSS patients did not. Two transporters involved in red cell dehydration, the conductive channels Psickle and the Gardos channel, behaved similarly in red cells from the two genotypes, but were significantly less active in HbSC patients. By contrast, KCC activity was quantitatively greater in HbSC red cells. Results suggest that KCC is likely to have greater involvement in red cell dehydration in HbSC patients, which could explain its association with disease severity in this genotype. This work supports the hypothesis that SCD in HbSC patients is a distinct disease entity to that in HbSS patients. Results suggest the possibility of designing specific treatments of particular benefit to HbSC patients and a rationale for the development of prognostic markers, to inform early treatment of children likely to develop more severe complications of the disease.

  4. The clinical application value of glycosylated hemoglobin(HbA1c) plus fasting plasma glucose in diagnosing diabetes

    Institute of Scientific and Technical Information of China (English)

    程多智

    2012-01-01

    Objective To evaluate clinical application value of HbA1c plus fasting plasma glucose(FPG) in diagnosing diabetes mellitus(DM).Methods 681 patients with DM were enrolled in this study. The level of HbA1c by Bio-RAD-10 glycated hemoglobin analyzer,FPG by OLYMPUS

  5. New diagnostic criteria for diabetes: is the change from glucose to HbA1c possible in all populations?

    DEFF Research Database (Denmark)

    Jørgensen, Marit Eika; Bjerregaard, Peter; Borch-Johnsen, Knut;

    2010-01-01

    Recently, a change of the diagnostic tool for diabetes from an oral glucose tolerance test (OGTT) to hemoglobin A1c (HbA1c) has been suggested. The aim of the study was to assess whether ethnicity modified the association between glucose levels and HbA1c and to compare diabetes prevalence according...

  6. Determination of the value of glycated hemoglobin HbA1c and fructosamine in assessing the risk of perioperative complications after cardiac surgery in patients with type 2 diabetes.

    Science.gov (United States)

    Kowalczuk-Wieteska, Anetta M; Wróbel, Marta; Rokicka, Dominika; Szymborska-Kajanek, Aleksandra; Foremny, Jerzy; Nadziakiewicz, Paweł; Zembala, Marian; Strojek, Krzysztof

    2016-12-01

    Patients with diabetes have a worse postoperative course and longer length of hospital stay after surgery. A good indicator of proper long-term (3 months) glycemic control is glycated hemoglobin (HbA1c), and fructosamine in the short term (2-3 weeks). To determine the degree of glycemic control evaluated preoperatively by HbA1c and/or fructosamine influence on the postoperative course of patients with diabetes undergoing coronary artery bypass grafting (CABG) in 2014-2015. Before the operation HbA1c (N < 7.0) and fructosamine (N < 280 µmol/l) were measured and depending on the results the respondents were divided into 4 groups: group I (n = 46) - normal both parameters; group II (n = 22) - high both values; group III (n = 4) - normal fructosamine/HbA1c high; group IV (n = 33) - high HbA1c/fructosamine normal. Statistical analysis was performed using the t-test assuming p < 0.05 to be statistically significant. One hundred and five patients were treated by CABG/OPCAB (39 female, 66 males). The mean age was 65.7 ±7.3, HbA1c: 7.23 ±1.2%, fructosamine: 261.8 ±43.8. There was no difference in the incidence of other postoperative complications between the two groups. Glycated hemoglobin and fructosamine levels to a similar extent define the risk of perioperative complications in patients undergoing cardiac surgery. In patients in whom there is a need to quickly compensate for elevated blood glucose consider enabling determination of fructosamine.

  7. Determination of the value of glycated hemoglobin HbA1c and fructosamine in assessing the risk of perioperative complications after cardiac surgery in patients with type 2 diabetes

    Science.gov (United States)

    Wróbel, Marta; Rokicka, Dominika; Szymborska-Kajanek, Aleksandra; Foremny, Jerzy; Nadziakiewicz, Paweł; Zembala, Marian; Strojek, Krzysztof

    2016-01-01

    Introduction Patients with diabetes have a worse postoperative course and longer length of hospital stay after surgery. A good indicator of proper long-term (3 months) glycemic control is glycated hemoglobin (HbA1c), and fructosamine in the short term (2–3 weeks). Aim To determine the degree of glycemic control evaluated preoperatively by HbA1c and/or fructosamine influence on the postoperative course of patients with diabetes undergoing coronary artery bypass grafting (CABG) in 2014–2015. Material and methods Before the operation HbA1c (N < 7.0) and fructosamine (N < 280 µmol/l) were measured and depending on the results the respondents were divided into 4 groups: group I (n = 46) – normal both parameters; group II (n = 22) – high both values; group III (n = 4) – normal fructosamine/HbA1c high; group IV (n = 33) – high HbA1c/fructosamine normal. Statistical analysis was performed using the t-test assuming p < 0.05 to be statistically significant. Results One hundred and five patients were treated by CABG/OPCAB (39 female, 66 males). The mean age was 65.7 ±7.3, HbA1c: 7.23 ±1.2%, fructosamine: 261.8 ±43.8. There was no difference in the incidence of other postoperative complications between the two groups. Conclusions Glycated hemoglobin and fructosamine levels to a similar extent define the risk of perioperative complications in patients undergoing cardiac surgery. In patients in whom there is a need to quickly compensate for elevated blood glucose consider enabling determination of fructosamine. PMID:28096825

  8. Differential Effects of Personal-Level vs Group-Level Racial Discrimination on Health among Black Americans.

    Science.gov (United States)

    Hagiwara, Nao; Alderson, Courtney J; Mezuk, Briana

    2016-07-21

    Racial/ethnic minorities in the United States not only experience discrimination personally but also witness or hear about fellow in-group members experiencing discrimination (ie, group-level discrimination). The objective of our study was to examine whether the effects of group-level discrimination on mental and physical health are different from those of personal-level discrimination among Black Americans by drawing upon social psychology research of the Personal/Group Discrimination Discrepancy. We conducted a secondary analysis of cross-sectional survey data from a larger study. One hundred and twenty participants, who self-identified as Black/African Americans during the laboratory sessions (57.5% women, mean age = 48.97, standard deviation = 8.58) in the parent study, were included in our analyses. Perceived personal-level discrimination was assessed with five items that were taken from two existing measures, and group-level racial discrimination was assessed with three items. Self-reported physical and mental health were assessed with a modified version of SF-8. Perceived personal-level racial discrimination was associated with worse mental health. In contrast, perceived group-level racial discrimination was associated with better mental as well as physical health. Perceived group-level racial discrimination may serve as one of several health protective factors even when individuals perceive personal-level racial discrimination. The present findings demonstrate the importance of examining both personal- and group-level experiences of racial discrimination as they independently relate to health outcomes for Black Americans.

  9. Origin of Hb A2' (Hb B2) [delta16(A13)Gly --> Arg (GGC --> CGC)].

    Science.gov (United States)

    Bennani, Mohcine; Mombo, Landry-Erik; Chaventre, André; Barakat, Amina; Ducrocq, Rolande; Nagel, Ronald L; Krishnamoorthy, Rajagopal

    2003-05-01

    On a field trip to the Dogon country (le Pays Dogon) in central Mali, we detected a high frequency of the Hb A2 abnormality, reaching higher numbers among blacksmiths (up to 12.4%) living in the same villages. In this report, by direct nucleotide sequencing and employing a polymerase chain reaction-restriction fragment length polymorphism approach, we show that the Hb A2 variant observed in the Dogon population is indeed Hb A2', also called Hb B2, and that in all of the cases the abnormal delta-globin gene is linked to a unique haplotype. The same haplotype was found linked to Hb A2' in the Herero population belonging to the South African Bantu-speaking Blacks from Namibia. Although the unique origin of this mutation in Africa is a possibility, a recurrent mutational event cannot be excluded because the linked beta cluster haplotype is one of the two major haplotypes found in all African populations. A study of populations from other regions of Africa is required to clarify this issue.

  10. Differential thermal stability and oxidative vulnerability of the hemoglobin variants, HbA2 and HbE

    National Research Council Canada - National Science Library

    Chakrabarti, Abhijit; Bhattacharya, Dipankar; Deb, Sanghamitra; Chakraborty, Madhumita

    2013-01-01

    .... From absorption measurements in the soret region, synchronous fluorescence spectroscopy and dynamic light scattering experiments, we have found thermal stability of the three hemoglobin variants following the order HbEA...

  11. Differential Thermal Stability and Oxidative Vulnerability of the Hemoglobin Variants, HbA2 and HbE: e81820

    National Research Council Canada - National Science Library

    Abhijit Chakrabarti; Dipankar Bhattacharya; Sanghamitra Deb; Madhumita Chakraborty

    2013-01-01

    .... From absorption measurements in the soret region, synchronous fluorescence spectroscopy and dynamic light scattering experiments, we have found thermal stability of the three hemoglobin variants following the order HbEA...

  12. Estimation of the quantum efficiency of the photodissociation of HbO2 and HbCO

    Science.gov (United States)

    Gisbrecht, A. I.; Mamilov, S. A.; Esman, S. S.; Asimov, M. M.

    2016-01-01

    The paper presents our results on the study of the efficiency of inter-fractional changes in hemoglobin molecules depending on the laser radiation parameters. The evaluation of the quantum efficiency of light interaction in vivo with oxyhemoglobin (HbO2) and carboxyhemoglobin (HbCO) in the blood at wavelengths for 525 and 605 nm is presented. The photodissociation yield of 11% for HbO2 and 79% for HbCO are measured at the wavelength of 525 nm and 10 % for HbO2 and 76 % for HbCO at a wavelength of 605 nm. Thus, the quantum yield of photodissociation of the HbCO is considerably higher, which ensures high efficiency of photodecomposition of the HbCO in the blood. The obtained results can be used in the clinical phototherapy practice for effective treatment of CO poisoning.

  13. A multilevel perspective on faultlines: Differentiating the effects between group- and organizational-level faultlines.

    Science.gov (United States)

    Bezrukova, Katerina; Spell, Chester S; Caldwell, David; Burger, Jerry M

    2016-01-01

    Integrating the literature on faultlines, conflict, and pay, we drew on the basic principles of multilevel theory and differentiated between group- and organizational-level faultlines to introduce a novel multilevel perspective on faultlines. Using multisource, multilevel data on 30 Major League Baseball (MLB) teams, we found that group-level faultlines were negatively associated with group performance, and that internally focused conflict exacerbated but externally focused conflict mitigated this effect. Organizational-level faultlines were negatively related to organizational performance, and were most harmful in organizations with high levels of compensation. Implications for groups and teams in the sports/entertainment and other industries are discussed.

  14. Measurements of HbA1c by High Performance Liquid Chromatography in D-10 analyzer and Immunological Method by Beckman Coulter AU480 System: A Comparative Study

    Directory of Open Access Journals (Sweden)

    Md Aminul Haque Khan

    2012-07-01

    Full Text Available Background: HbA1c can be measured by different methods. Enzymatic, boronate affinity chromatographic and cation-exchange high performance liquid chromatographic (HPLC methods are considered as gold standard methods. To make the test cost-effective many laboratories use the immunological method installed in a chemistry analyzer. Objective: To compare the values of HbA1c measured by high performance liquid chromatographic method and immunological method in two laboratory settings. Materials and Methods: This observational and comparative study was conducted in Bangladesh Institute of Health Sciences (BIHS and Enam Medical College, Savar, Dhaka. HbA1c levels measured by HPLC in BIHS were compared with that measured by immunological method in Enam Medical College, Savar, Dhaka. p values 10.0% group, but it was within acceptable limit. Conclusion: HbA1c can be measured by immunological method installed in an automated chemistry analyzer to make the test cost-effective. But HPLC method is preferable.

  15. Hb A1c Separation by High Performance Liquid Chromatography in Hemoglobinopathies

    Science.gov (United States)

    Chandrashekar, Vani

    2016-01-01

    Hb A1c measurement is subject to interference by hemoglobin traits and this is dependent on the method used for determination. In this paper we studied the difference between Hb A1c measured by HPLC in hemoglobin traits and normal chromatograms. We also studied the correlation of Hb A1c with age. Hemoglobin analysis was carried out by high performance liquid chromatography. Spearman's rank correlation was used to study correlation between A1c levels and age. Mann-Whitney U test was used to study the difference in Hb A1c between patients with normal hemoglobin and hemoglobin traits. A total of 431 patients were studied. There was positive correlation with age in patients with normal chromatograms only. No correlation was seen in Hb E trait or beta thalassemia trait. No significant difference in Hb A1c of patients with normal chromatograms and patients with hemoglobin traits was seen. There is no interference by abnormal hemoglobin in the detection of A1c by high performance liquid chromatography. This method cannot be used for detection of A1c in compound heterozygous and homozygous disorders. PMID:26989559

  16. Hb A1c Separation by High Performance Liquid Chromatography in Hemoglobinopathies

    Directory of Open Access Journals (Sweden)

    Vani Chandrashekar

    2016-01-01

    Full Text Available Hb A1c measurement is subject to interference by hemoglobin traits and this is dependent on the method used for determination. In this paper we studied the difference between Hb A1c measured by HPLC in hemoglobin traits and normal chromatograms. We also studied the correlation of Hb A1c with age. Hemoglobin analysis was carried out by high performance liquid chromatography. Spearman’s rank correlation was used to study correlation between A1c levels and age. Mann-Whitney U test was used to study the difference in Hb A1c between patients with normal hemoglobin and hemoglobin traits. A total of 431 patients were studied. There was positive correlation with age in patients with normal chromatograms only. No correlation was seen in Hb E trait or beta thalassemia trait. No significant difference in Hb A1c of patients with normal chromatograms and patients with hemoglobin traits was seen. There is no interference by abnormal hemoglobin in the detection of A1c by high performance liquid chromatography. This method cannot be used for detection of A1c in compound heterozygous and homozygous disorders.

  17. Evaluation of the reliability of RBC indices in discriminating between Hb H disease and iron deifciency anemia in infants%红细胞指数鉴别婴幼儿血红蛋白H病和缺铁性贫血的作用评价

    Institute of Scientific and Technical Information of China (English)

    陈剑锋; 陈一菁; 梁婉怡; 兰和魁; 龙桂芳

    2013-01-01

    Objectives To evaluate the reliability of RBC indices in discriminating between Hb H disease and iron deficiency anemia (IDA) in infants.Methods Seventy-seven patients with Hb H disease and 108 patients with IDA were enrolled in this study. RBC indices were calculated by 7 kinds of formulas. Sensitivity, specificity and Youden's index of these RBC indices for predicting Hb H were also evaluated. Results Comparing the patients' RBC parameters between Hb H disease and IDA groups, high RBC levels were found in the former while highe MCV and MCH in the latter. However, there was no significant difference in Hb level between the two groups. Among seven RBC indices, Green and King Index (GKI) showed the highest sensitivity (90.7%) and Youden's index (80.3%). Conclusion Seven kinds of formulas for RBC index calculation can be used to discriminate between Hb H disease and IDA, and GKI index appears most reliable among them.%目的:评估红细胞指数鉴别婴幼儿中间型α地中海贫血(Hb H病)和缺铁性贫血患儿的作用。方法77例Hb H病及108例缺铁性贫血婴幼儿纳入研究。采用7种公式计算红细胞指数,分析其对Hb H病的预测敏感度、特异性和Youden's指数。结果 Hb H病红细胞比缺铁性贫血高,红细胞平均体积、红细胞平均血红蛋白比后者低,两组患儿的血红蛋白水平差异无显著意义。7种指数中,Green and King指数(GKI)对Hb H病的预测敏感度(90.7%)及Youden's指数(80.3%)最高。结论红细胞指数计算公式可应用于鉴别Hb H病和缺铁性贫血,其中GKI指数可靠性相对较高。

  18. Calling all stakeholders: group-level assessment (GLA)-a qualitative and participatory method for large groups.

    Science.gov (United States)

    Vaughn, Lisa M; Lohmueller, MaryAnn

    2014-08-01

    Group-level assessment (GLA) is a qualitative and participatory large group method in which timely and valid data are collaboratively generated and interactively evaluated with relevant stakeholders leading to the development of participant-driven data and relevant action plans. This method is useful across a wide range of evaluation purposes in many environments. GLA involves bringing a large group of participants together to build a common database through the co-identification of relevant needs, judgments, and priorities. The GLA process proceeds through the following seven steps: climate setting, generating, appreciating, reflecting, understanding, selecting, and action. This article describes the methodological development and process of conducting a GLA and its various applications across the evaluation spectrum. We highlight several exemplars where GLA was used in order to demonstrate the particular nuances of working with different sizes and types of groups and to elaborate on our learnings from the wide applicability of the method.

  19. Relationship of biomedical science content acquisition performance to students' level of PBL group interaction: are students learning during PBL group?

    Science.gov (United States)

    Romito, Laura M; Eckert, George J

    2011-05-01

    This study assessed biomedical science content acquisition from problem-based learning (PBL) and its relationship to students' level of group interaction. We hypothesized that learning in preparation for exams results primarily from individual study of post-case learning objectives and that outcomes would be unrelated to students' group involvement. During dental curricular years 1 and 2, student-generated biomedical learning issues (LIs) were identified from six randomly chosen PBL cases. Knowledge and application of case concepts were assessed with quizzes based on the identified LIs prior to dissemination of the learning objectives. Students and facilitators were surveyed on students' level of group involvement for the assessed LI topics. Year 1 students had significantly higher assessment scores (p=0.0001). For both student classes, means were significantly higher for the recall item (Q1) than for the application item (Q2). Q1 scores increased along with the student's reported role for Year 1 (p=0.04). However, there was no relationship between the student's reported role and Q1 for Year 2 (p=0.20). There was no relationship between the student's reported role and Q2 for Year 1 (p=0.09) or Year 2 (p=0.19). This suggests that students' level of group involvement on the biomedical learning issues did not significantly impact students' assessment performance.

  20. Hb H disease resulting from the association of an αº-thalassemia allele [-(α20.5] with an unstable α-globin variant [Hb Icaria]: first report on the occurrence in Brazil

    Directory of Open Access Journals (Sweden)

    Elza M. Kimura

    2009-01-01

    Full Text Available Hb H Disease is caused by the loss or inactivation of three of the four functional a-globin genes. Patients present chronic hemolytic anemia and splenomegaly. In some cases, occasional blood transfusions are required. Deletions are the main cause of this type of thalassemia (α-thalassemia. We describe here an unusual case of Hb H disease caused by the combination of a common αº deletion [-(α20.5] with a rare point mutation (c.427T > A, thus resulting in an elongated and unstable α-globin variant, Hb Icaria, (X142K, with 31 additional amino-acid residues. Very high levels of Hb H and Hb Bart's were detected in the patient's red blood cells (14.7 and 19.0%, respectively. This is the first description of this infrequent association in the Brazilian population.

  1. Designs for the combination of group- and individual-level data.

    Science.gov (United States)

    Haneuse, Sebastien; Bartell, Scott

    2011-05-01

    Studies of ecologic or aggregate data suffer from a broad range of biases when scientific interest lies with individual-level associations. To overcome these biases, epidemiologists can choose from a range of designs that combine these group-level data with individual-level data. The individual-level data provide information to identify, evaluate, and control bias, whereas the group-level data are often readily accessible and provide gains in efficiency and power. Within this context, the literature on developing models, particularly multilevel models, is well-established, but little work has been published to help researchers choose among competing designs and plan additional data collection. We review recently proposed "combined" group- and individual-level designs and methods that collect and analyze data at 2 levels of aggregation. These include aggregate data designs, hierarchical related regression, two-phase designs, and hybrid designs for ecologic inference. The various methods differ in (i) the data elements available at the group and individual levels and (ii) the statistical techniques used to combine the 2 data sources. Implementing these techniques requires care, and it may often be simpler to ignore the group-level data once the individual-level data are collected. A simulation study, based on birth-weight data from North Carolina, is used to illustrate the benefit of incorporating group-level information. Our focus is on settings where there are individual-level data to supplement readily accessible group-level data. In this context, no single design is ideal. Choosing which design to adopt depends primarily on the model of interest and the nature of the available group-level data.

  2. Unbiased Group-Level Statistical Assessment of Independent Component Maps by Means of Automated Retrospective Matching

    NARCIS (Netherlands)

    Langers, Dave R. M.

    2010-01-01

    This report presents and validates a method for the group-level statistical assessment of independent component analysis (ICA) outcomes. The method is based on a matching of individual component maps to corresponding aggregate maps that are obtained from concatenated data. Group-level statistics are

  3. Influence of Erythropoiesis-Stimulating Agents on HbA1c and Fructosamine in Patients with Haemodialysis.

    Science.gov (United States)

    Rasche, Franz Maximilian; Ebert, Thomas; Beckmann, Julia; Busch, Volker; Barinka, Filip; Rasche, Wilma Gertrud; Lindner, Tom H; Schneider, Jochen G; Schiekofer, Stephan

    2017-06-01

    HbA1c is the most accepted laboratory parameter for the long term observation of glucose control. There is still much of a debate about the use of HbA1c as a metabolic indicator in diabetic patients (DM) on haemodialysis (HD) and erythropoiesis-stimulating agent (ESA) therapy because of the altered erythrocyte turn over in patients with chronic kidney disease and haemodialysis (CKD5D). In 102 CKD5 patients with and without diabetes mellitus, we examined the dose dependent variability in HbA1c and fructosamine levels under haemodialysis and treated with epoetin α (n=48) and a new generation agent with continuous stimulation of methoxy polyethylene glycol epoetin beta (C.E.R.A.; n=54). HbA1c levels were affected by therapy with ESA treatments. ESA dose was inversely correlated with HbA1c and an escalation of 10.000 IU per week induced an estimated decrease of HbA1c of 0.6 percent. In addition, the increase of reticulocyte number as a marker for erythropoiesis was significantly inversely correlated with the increase of ΔHbA1c. ESA treatments had no such effect on the alternative metabolic parameter fructosamine. When compared, both therapeutic agents had comparable success in attaining haemoglobin (Hb) target values. C.E.R.A. showed better correlation and was more effective over a longer dose interval. Our results show that HbA1c levels in patients should be carefully interpreted based on interfering factors. Nevertheless, HbA1c is currently the most consistent parameter for use ascertaining metabolic status of patients suffering from diabetes mellitus. © Georg Thieme Verlag KG Stuttgart · New York.

  4. Hydroxyurea decreases hospitalizations in pediatric patients with Hb SC and Hb SB+ thalassemia

    Directory of Open Access Journals (Sweden)

    Lebensburger JD

    2015-12-01

    Full Text Available Jeffrey D Lebensburger, Rakeshkumar J Patel, Prasannalaxmi Palabindela, Christina J Bemrich-Stolz, Thomas H Howard, Lee M HilliardDivision of Pediatric Hematology Oncology, University of Alabama at Birmingham, Birmingham, AL, USAPurpose: Patients with hemoglobin SC (Hb SC and hemoglobin SB+ (Hb SB+ thalassemia suffer from frequent hospitalizations yet strong evidence of a clinical benefit of hydroxyurea (HU in this population is lacking. Patients with recurrent hospitalizations for pain crisis are offered HU at our institution based on small cohort data and anecdotal benefit. This study identifies outcomes from a large cohort of patients with Hb SC and SB+ thalassemia who were treated with HU for 2 years.Materials and methods: A retrospective review was conducted of 32 patients with Hb SC and SB+ thalassemia who were treated with HU. We reviewed the number, and reasons for hospitalization in the 2 years prior to, and 2 years post-HU treatment as well as laboratory changes from baseline, over 1 year.Results: Patients with Hb SC and SB+ thalassemia started on HU for frequent pain, had a significant reduction in hospitalizations over 2 years as compared to the 2 years prior to HU initiation (mean total hospitalizations/year: pre-HU: 1.6 vs post-HU 0.4 hospitalizations, P<0.001; mean pain hospitalizations/year: pre-HU 1.5 vs post-HU 0.3 hospitalizations, P<0.001. Patients demonstrated hematologic changes including an increase in percent fetal hemoglobin (%HbF pre–post HU (4.5% to 7.7%, P=0.002, mean corpuscular volume (74 to 86 fL, P<0,0001, and decrease in absolute neutrophil count (5.0 to 3.2×109/L, P=0.007. Patients with higher doses of HU demonstrated the greatest reduction in hospitalizations but this was unrelated to absolute neutrophil count.Conclusion: This cohort of patients with Hb SC and SB+ thalassemia provides additional support for using HU in patients with recurrent hospitalizations for pain. A large randomized multicenter trial of

  5. [Evaluation of HbA1c using different methods in haemoglobin variant, Hb J-Bangkok].

    Science.gov (United States)

    Sawaragi, Wakana; Shibuya, Hitoshi; Yasuda, Keiko; Suzuki, Haruki; Shimizu, Chikara; Matsuno, Kazuhiko

    2009-05-01

    A 31-year-old Japanese man with haemoglobin variant, Hb J-Bangkok [beta56 (D7) Gly-->Asp], was found by discrepant values between HbA1c and glycated-albumin. We measured HbA1c using three different methods, HPLC, enzyme assay and turbidimetric immunoassay. HbA1c value measured by HPLC was much lower than those by others. Furthermore, we estimated calculated glyco-haemoglobin value measured by high-resolution HPLC, revealing that HbA1c values measured by enzyme assay and turbidimetric immunoassay were comparable with calculated value. When measuring HbA1c value in haemoglobin variant, Hb J Bangkok, enzyme assay and turbidimetric immunoassay are useful methods.

  6. Evaluation of Intelligent Grouping Based on Learners' Collaboration Competence Level in Online Collaborative Learning Environment

    Science.gov (United States)

    Muuro, Maina Elizaphan; Oboko, Robert; Wagacha, Waiganjo Peter

    2016-01-01

    In this paper we explore the impact of an intelligent grouping algorithm based on learners' collaborative competency when compared with (a) instructor based Grade Point Average (GPA) method level and (b) random method, on group outcomes and group collaboration problems in an online collaborative learning environment. An intelligent grouping…

  7. Molecular concentration of deoxyHb in human prefrontal cortex predicts the emergence and suppression of consciousness.

    Science.gov (United States)

    Leon-Dominguez, Umberto; Izzetoglu, Meltem; Leon-Carrion, Jose; Solís-Marcos, Ignacio; Garcia-Torrado, Francisco Jose; Forastero-Rodríguez, Ana; Mellado-Miras, Patricia; Villegas-Duque, Diego; Lopez-Romero, Juan Luis; Onaral, Banu; Izzetoglu, Kurtulus

    2014-01-15

    This is the first study to use fNIRS to explore anaesthetic depth and awakening during surgery with general anaesthesia. A 16 channel continuous wave (CW) functional near-infrared system (fNIRS) was used to monitor PFC activity. These outcomes were compared to BIS measures. The results indicate that deoxyHb concentration in the PFC varies during the suppression and emergence of consciousness. During suppression, deoxyHb levels increase, signalling the deactivation of the PFC, while during emergence, deoxyHb concentration drops, initiating PFC activation and the recovery of consciousness. Furthermore, BIS and deoxyHb concentrations in the PFC display a high negative correlation throughout the different anaesthetic phases. These findings suggest that deoxyHb could be a reliable marker for monitoring anaesthetic depth, and that the PFC intervenes in the suppression and emergence of consciousness. © 2013 Elsevier Inc. All rights reserved.

  8. Individual and group-level job resources and their relationships with individual work engagement.

    Science.gov (United States)

    Füllemann, Désirée; Brauchli, Rebecca; Jenny, Gregor J; Bauer, Georg F

    2016-06-16

    This study adds a multilevel perspective to the well-researched individual-level relationship between job resources and work engagement. In addition, we explored whether individual job resources cluster within work groups because of a shared psychosocial environment and investigated whether a resource-rich psychosocial work group environment is beneficial for employee engagement over and above the beneficial effect of individual job resources and independent of their variability within groups. Data of 1,219 employees nested in 103 work groups were obtained from a baseline employee survey of a large stress management intervention project implemented in six medium and large-sized organizations in diverse sectors. A variety of important job resources were assessed and grouped to an overall job resource factor with three subfactors (manager behavior, peer behavior, and task-related resources). Data were analyzed using multilevel random coefficient modeling. The results indicated that job resources cluster within work groups and can be aggregated to a group-level job resources construct. However, a resource-rich environment, indicated by high group-level job resources, did not additionally benefit employee work engagement but on the contrary, was negatively related to it. On the basis of this unexpected result, replication studies are encouraged and suggestions for future studies on possible underlying within-group processes are discussed. The study supports the presumed value of integrating work group as a relevant psychosocial environment into the motivational process and indicates a need to further investigate emergent processes involved in aggregation procedures across levels.

  9. Significance of HbA1c and its measurement in the diagnosis of diabetes mellitus: US experience

    Directory of Open Access Journals (Sweden)

    Juarez DT

    2014-10-01

    Full Text Available Deborah Taira Juarez, Kendra M Demaris, Roy Goo, Christina Louise Mnatzaganian, Helen Wong SmithDaniel K Inouye College of Pharmacy, University of Hawaii at Hilo, Honolulu, HI, USAAbstract: The 2014 American Diabetes Association guidelines denote four means of diagnosing diabetes. The first of these is a glycosylated hemoglobin (HbA1c >6.5%. This literature review summarizes studies (n=47 in the USA examining the significance, strengths, and limitations of using HbA1c as a diagnostic tool for diabetes, relative to other available means. Due to the relatively recent adoption of HbA1c as a diabetes mellitus diagnostic tool, a hybrid systematic, truncated review of the literature was implemented. Based on these studies, we conclude that HbA1c screening for diabetes has been found to be convenient and effective in diagnosing diabetes. HbA1c screening is particularly helpful in community-based and acute care settings where tests requiring fasting are not practical. Using HbA1c to diagnose diabetes also has some limitations. For instance, HbA1c testing may underestimate the prevalence of diabetes, particularly among whites. Because this bias differs by racial group, prevalence and resulting estimates of health disparities based on HbA1c screening differ from those based on other methods of diagnosis. In addition, existing evidence suggests that HbA1c screening may not be valid in certain subgroups, such as children, women with gestational diabetes, patients with human immunodeficiency virus, and those with prediabetes. Further guidelines are needed to clarify the appropriate use of HbA1c screening in these populations.Keywords: diabetes mellitus, diagnosis, glycosylated hemoglobin, USA

  10. Identification, Functional Study, and Promoter Analysis of HbMFT1, a Homolog of MFT from Rubber Tree (Hevea brasiliensis).

    Science.gov (United States)

    Bi, Zhenghong; Li, Xiang; Huang, Huasun; Hua, Yuwei

    2016-03-02

    A homolog of MOTHER OF FT AND TFL1 (MFT) was isolated from Hevea brasiliensis and its biological function was investigated. Protein multiple sequence alignment and phylogenetic analysis revealed that HbMFT1 conserved critical amino acid residues to distinguish MFT, FLOWERING LOCUS T (FT) and TERMINAL FLOWER1 (TFL1)-like proteins and showed a closer genetic relationship to the MFT-like group. The accumulation of HbMFT1 was generally detected in various tissues except pericarps, with the highest expression in embryos and relatively higher expression in roots and stems of seedlings, flowering inflorescences, and male and female flowers. HbMFT1 putative promoter analysis showed that tissue-specific, environmental change responsive and hormone-signaling responsive elements were generally present. HbMFT1 was strongly induced under a short-day condition at 28 °C, with the highest expression after the onset of a day. Overexpression of HbMFT1 inhibited seed germination, seedling growth, and flowering in transgenic Arabidopsis. The qRT-PCR further confirmed that APETALA1 (AP1) and FRUITFULL (FUL) were drastically down-regulated in 35S::HbMFT1 plants. A histochemical β-glucuronidase (GUS) assay showed that HbMFT1::GUS activity was mainly detected in stamens and mature seeds coinciding with its original expression and notably induced in rosette leaves and seedlings of transgenic Arabidopsis by exogenous abscisic acid (ABA) due to the presence of ABA cis-elements in HbMFT1 promoter. These results suggested that HbMFT1 was mainly involved in maintenance of seed maturation and stamen development, but negatively controlled germination, growth and development of seedlings and flowering. In addition, the HbMFT1 promoter can be utilized in controlling transgene expression in stamens and seeds of rubber tree or other plant species.

  11. Identification, Functional Study, and Promoter Analysis of HbMFT1, a Homolog of MFT from Rubber Tree (Hevea brasiliensis

    Directory of Open Access Journals (Sweden)

    Zhenghong Bi

    2016-03-01

    Full Text Available A homolog of MOTHER OF FT AND TFL1 (MFT was isolated from Hevea brasiliensis and its biological function was investigated. Protein multiple sequence alignment and phylogenetic analysis revealed that HbMFT1 conserved critical amino acid residues to distinguish MFT, FLOWERING LOCUS T (FT and TERMINAL FLOWER1 (TFL1-like proteins and showed a closer genetic relationship to the MFT-like group. The accumulation of HbMFT1 was generally detected in various tissues except pericarps, with the highest expression in embryos and relatively higher expression in roots and stems of seedlings, flowering inflorescences, and male and female flowers. HbMFT1 putative promoter analysis showed that tissue-specific, environmental change responsive and hormone-signaling responsive elements were generally present. HbMFT1 was strongly induced under a short-day condition at 28 °C, with the highest expression after the onset of a day. Overexpression of HbMFT1 inhibited seed germination, seedling growth, and flowering in transgenic Arabidopsis. The qRT-PCR further confirmed that APETALA1 (AP1 and FRUITFULL (FUL were drastically down-regulated in 35S::HbMFT1 plants. A histochemical β-glucuronidase (GUS assay showed that HbMFT1::GUS activity was mainly detected in stamens and mature seeds coinciding with its original expression and notably induced in rosette leaves and seedlings of transgenic Arabidopsis by exogenous abscisic acid (ABA due to the presence of ABA cis-elements in HbMFT1 promoter. These results suggested that HbMFT1 was mainly involved in maintenance of seed maturation and stamen development, but negatively controlled germination, growth and development of seedlings and flowering. In addition, the HbMFT1 promoter can be utilized in controlling transgene expression in stamens and seeds of rubber tree or other plant species.

  12. 糖化血红蛋白、果糖胺测定在妊娠糖尿病中的应用价值%Application Value of HbA1c and Single glycosaminoglycan Detection in the Gestational Diabetes

    Institute of Scientific and Technical Information of China (English)

    徐晔

    2012-01-01

    Objective To investigate the feasibility and clinical significance of glycated hemoglobin (HbAlc) and single glycosaminogiycan (FRU) in the determination of gestational diabetes screening.Methods 50 g diabetes glucose screening test (GCT) and HbAlc level,concentration levels of the FRU were analysis in 76 cases of normal pregnancy,25 cases of gestational impaired glucose tolerance patients,50 cases of gestational diabetes. OGTT test was as the gold standard in diagnostic tests of GDM. Results HbAlc and fructosamine levels in the blood of the gestational diabetes group was significantly higher than the normal pregnant group and gestational impaired glucose tolerance group. The FRU value was significantly increased with the age of patients. The level of HbAlc increased with age changes. Conclusion HbAlc and fructosamine can be diagnostic indicators as gestational diabetes screening.%目的 探讨糖化血红蛋白(HbAlc)果糖胺(FRU)在妊娠糖尿病筛查中的可行性及临床意义.方法 分析76例正常妊娠孕妇、25例妊娠期糖耐量受损患者,50例妊娠期糖尿病患者50 g糖尿病葡萄糖筛查试验(GCT)和HbAlc、FRU含量水平,以OGTT诊断试验作为GDM的金标准.结果 妊娠糖尿病组血中HbAlc和果糖胺水平明显高于正常孕妇组及妊娠期糖耐量受损组.FRU值随年龄呈显著性增值改变,HbAlc随年龄的增高呈降低改变.结论 HbAlc和果糖胺可以作为妊娠期糖尿病筛查诊断的指标.

  13. Sickle cell trait is associated with controlled levels of haem and mild proinflammatory response during acute malaria infection

    Science.gov (United States)

    Ademolue, T. W.; Amodu, O. K.

    2017-01-01

    Summary The controlled induction of haemoxygenase‐1 (HO‐1), an enzyme that catabolizes haem, has been shown to reduce haem, preventing pathologies associated with haem toxicity. The hemoglobin genotype HbAS confers reduced susceptibility to severe complications of malaria by a mechanism that is not well understood. Using a longitudinal approach, we investigated the effect of baseline concentrations of HO‐1 on the accumulation of haem during acute Plasmodium falciparum malaria in HbAS and HbAA genotypes. Plasma concentrations of haem, HO‐1 and cytokines were quantified in venous blood obtained from children (9 months–5 years of age) during malaria infection, and at convalescence (baseline levels). Parasitaemia was determined during malaria infection. In patients with the HbAA genotype, there was a significant elevation in the plasma concentration of haem (P = 0.002), and a consequent increased induction of HO‐1 (P < 0.001) during falciparum malaria compared with levels at convalescence. Contrary to HbAA, plasma concentration of haem did not change in the HbAS genotypical group (P = 0·110), and the induction of HO‐1 was reduced during malaria compared with levels at convalescence (P = 0·006). Higher plasma levels of haem were observed in HbAS compared with HbAA at convalescence (P = 0·010), but this difference did not affect the levels of HO‐1 within each genotype (P = 0·450). Relatively milder proinflammatory responses were observed in HbAS children during malaria infection compared to HbAA children. Our findings suggest that a mechanism of reduced susceptibility to severe malaria pathologies by the HbAS genotype may involve the control of haem, leading to controlled levels of HO‐1 and milder proinflammatory responses during acute malaria. PMID:28142190

  14. Explaining levels of within-group wage inequality in U.S. labor markets.

    Science.gov (United States)

    McCall, L

    2000-11-01

    Most research on earnings inequality has focused on the growing gap between workers of different races and at different education, age, and income levels, but a large portion of the increasing inequality has actually occurred within these groups. This article focuses on the extent and sources of "within-group" wage inequality in more than 500 labor markets in the United States in 1990. In addition to documenting that within-group wage inequality across regions varies more widely today than over the past several decades, the analysis reveals that two frequently cited explanations of rising wage inequality over time have little impact on within-group wage inequality when measured at the local labor market level: (1) industrial shifts and (2) increased technology and trade. By contrast, flexible and insecure employment conditions (e.g., unemployment, contingent work, and immigration) are associated strongly with high local levels of within-group wage inequality, especially among women.

  15. A network-level explanation for the differences in HIV prevalence in South Africa's racial groups

    National Research Council Canada - National Science Library

    Kenyon, Chris; Dlamini, Sipho; Boulle, Andrew; White, Richard G; Badri, Motasim

    Analyses of individual-level risk factors have not been able to adequately explain why HIV has spread so extensively in southern Africa and why this has occurred especially within certain racial or ethnic groups...

  16. 空腹血糖与糖化血红蛋白在不同性别和年龄间的差异%Differences of FPG and HbA1c in different genders and different ages

    Institute of Scientific and Technical Information of China (English)

    金宏伟; 黄回滨; 王欣欣

    2015-01-01

    目的:研究厦门地区不同年龄和性别人群空腹血糖(FPG)与糖化血红蛋白(HbA1c)水平的差异,并探讨该地区人群FPG、HbA1c水平与性别、年龄的关系。方法采用横断面研究方法,收集2013年1~12月符合要求的7754例体检者,抽取静脉血分别检测FPG与HbA1c水平,分析不同性别和年龄组之间 FPG、HbA1c水平的差异,以及FPG、HbA1c与性别、年龄的关系,并分析采用2010年美国糖尿病学会(ADA )糖尿病(DM )诊断标准的诊断结果。结果男性体检人群FPG与HbA1c水平均高于女性,比较差异均有统计学意义(P<0.05);不同性别人群的FPG、HbA1c水平均呈现随年龄增大而逐渐上升的趋势;相关性分析发现除18~30岁年龄组,其余年龄段的男性、女性及全部体检者 HbA1c与FPG水平均呈明显的正相关。依据2010年ADA标准,DM患者及高危人群分别占10.83%和43.65%。结论厦门地区人群FPG、HbA1c水平与年龄、性别均相关,提示有必要建立不同的参考区间和DM诊断切点;且2010年 ADA 标准可识别出更多的DM 患者及高危人群,尤其适合应用于体检筛查。%Objective To study the differences of fasting plasma glucose(FPG) and glycosylated hemoglobin A1c (HbA1c) in different genders and ages ,and to investigate the relationship between FPG and HbA1c levels with gender and age .Methods By adopting the cross‐sectional study method .7 754 cases of physical examination meeting the requirements in our hospital from Jan .2013 to Dec .2013 were recruited into this study .The venous blood sample was collected for detecting FPG and HbA1c .The differences of FPG and HbA1c levels in different gender and age groups were analyzed and the relationship between FPG and HbA1c levels with gender and age was analyzed .The di‐agnosis results by adopting the diabetes mellitus (DM ) criteria of American Diabetes Association in 2010 was ana

  17. Patterns of maltreatment and diagnosis across levels of care in group homes.

    Science.gov (United States)

    Pane Seifert, Heather T; Farmer, Elizabeth M Z; Wagner, H Ryan; Maultsby, Linda T; Burns, Barbara J

    2015-04-01

    Patterns of Axis I psychiatric diagnosis and maltreatment history were explored among youth in group homes, including match of clinical need to level or restrictiveness of care. Data on demographics, diagnoses, maltreatment, and group home level of care (Level I, II, or III homes, representing lower to higher intensity of supervision and treatment) were obtained from 523 youth who participated in a quasi-experimental study of group homes. Three quarters of youth had a diagnosis and two-thirds of youth had a maltreatment history. Youth in higher level homes had more diagnoses and higher rates of all disorders except adjustment disorders. Youth in Level I homes had a history of more maltreatment types, particularly high rates of neglect. Sexual abuse, physical abuse, and emotional abuse were most common among youth in higher level homes. Regardless of diagnosis history, comparable proportions of youth had a maltreatment history, and similar patterns were found across levels of care. Together, findings indicate that group homes with varying degrees of restrictiveness serve youth with different psychiatric diagnosis and maltreatment histories. Youth triaged to higher level homes had more diagnoses, while youth placed in the least restrictive homes had a history of more maltreatment subtypes. Further, distinct patterns of diagnosis types and maltreatment subtypes were seen across homes. Implications include the importance of assessing unique clinical needs of youth to promote an appropriate match to level of care and treatment plan. Copyright © 2015 Elsevier Ltd. All rights reserved.

  18. 77 FR 1778 - U.S.-EU High Level Working Group on Jobs and Growth

    Science.gov (United States)

    2012-01-11

    ... TRADE REPRESENTATIVE U.S.-EU High Level Working Group on Jobs and Growth AGENCY: Office of the United... Working Group on Jobs and Growth, led by U.S. Trade Representative Ron Kirk and EU Trade Commissioner... and investment to support mutually beneficial job creation, economic growth, and...

  19. Measurement Error Correction Formula for Cluster-Level Group Differences in Cluster Randomized and Observational Studies

    Science.gov (United States)

    Cho, Sun-Joo; Preacher, Kristopher J.

    2016-01-01

    Multilevel modeling (MLM) is frequently used to detect cluster-level group differences in cluster randomized trial and observational studies. Group differences on the outcomes (posttest scores) are detected by controlling for the covariate (pretest scores) as a proxy variable for unobserved factors that predict future attributes. The pretest and…

  20. The Clinical Usefulness of Serum Fructosamine and HbAlc in Patients with NIDDM

    Science.gov (United States)

    Lim, Si Young; Jhoo, Young Man; Lee, Seung Sei; Lee, Man Ho; Chung, Eul Soon; Lee, Sang Jong

    1989-01-01

    To evaluate the clinical values of serum fructosamine and HbAlc, serum fructosamine and HbAlc were assessed in 50 normal controls and 36 patients with NIDDM (non-insulin-dependent diabetes mellitus). And we studied 10 patients selected at random among the 36 patients with NIDDM. The values of serum fructosamine and HbAlc in controls were 2.1 mmol/L and 5.3%, respectively. The levels of serum fructosamine and HbAlc were significantly higher in NIDDM patients (3.27 mmol/L and 8.8%, respectively, p<0.005) compared with those in the controls. The serum fructosamine was significantly correlated with fasting plasma glucose and HbAlc (r=0.78, p<0.78, p<0.001; r=0.76, p<0.005, respectively). Also, in retrograde study, serum fructosamine concentration had a significant correlation to fasting plasma glucose determined 2 weeks before (r=0.72, p<0.002), and 4 weeks before (r=0.54, p<0.005). It is concluded that serum fructosamine measurement may be useful in monitoring short-term control of plasma glucose in patients with NIDDM. PMID:2486845

  1. Allocating Group-Level Payments for Ecosystem Services: Experiences from a REDD+ Pilot in Tanzania

    Directory of Open Access Journals (Sweden)

    Elizabeth J. Z. Robinson

    2016-12-01

    Full Text Available Payments for ecosystem services (PES typically reward landowners for managing their land to provide ecosystem services that would not otherwise be provided. REDD+—Reduced Emissions from Deforestation and Forest Degradation—is a form of PES aimed at decreasing carbon emissions from forest conversion and extraction in lower-income countries. A key challenge for REDD+ occurs when it is implemented at a group, rather than an individual landowner, level. Whilst achieving a group-level reduction relies on individuals changing their interaction with the forest, incentives are not aligned explicitly at the individual level. Rather, payments are made to a defined group as a single entity in exchange for verified reduced forest loss, as per a PES scheme. In this paper, we explore how REDD+ has been implemented in one multiple-village pilot in Tanzania with the village defining the group. Our findings suggest that considerable attention has been paid towards monitoring, reporting, verification (MRV, and equity. No explicit mechanism ensures individual compliance with the village-level PES, and few villages allocate funds for explicit enforcement efforts to protect the forest from illegal activities undertaken by individual group members or by outsiders. However, the development of village-level institutions, “social fencing,” and a shared future through equal REDD+ payments, factor into decisions that influence the level of compliance at the village level that the program will eventually achieve.

  2. Hb E-β-Thalassemia in Five Indian States.

    Science.gov (United States)

    Italia, Khushnooma; Dabke, Pooja; Sawant, Pratibha; Nadkarni, Anita; Ghosh, Kanjaksha; Colah, Roshan B

    2016-09-01

    Hb E [β26(B8)Glu→Lys; HBB: c.79G > A]-β-thalassemia (β-thal) has an extremely variable clinical presentation. We report the clinical features of these patients from five Indian states together with their hematological and molecular characteristics. Seventy-eight Hb E-β-thal patients from different regions [West Bengal (30), Maharashtra (21), Uttar Pradesh (13), Bihar (11), Orissa (3)] were clinically evaluated along with hematological profiles and molecular characteristics (β-thal mutations, XmnI polymorphisms, α genotypes). Twenty-nine of the 78 patients had a mild clinical presentation (clinical score 2.2 ± 1.1), while 15 had moderate severity (clinical score 6.1 ± 1.2) with occasional transfusion needs, and 34 patients were severely affected (clinical score 8.2 ± 0.5) requiring regular blood transfusions. The age at clinical presentation in the severely affected patients was lower (6 months-10 years) as compared to those with milder symptoms (2 years-34 years). Thirty-four patients showed splenomegaly (spleen ≥3 cm below the costal margin) and five patients were splenectomized. The severe β(+ )IVS1-5 (G > C) (HBB: c.92 + 5G > C) was the most common β-thal mutation, while seven other mutations were also seen. The XmnI [+/+] and [-/-] polymorphisms were seen in 24.1 and 10.3% of mildly affected patients and 14.7 and 17.6% of severely affected patients respectively. A single α gene deletion (-α(3.7)/αα) was found in 20.7% of mildly affected and 5.9% of severely affected patients, respectively. No specific differences in the clinical, hematological or molecular characteristics were observed in the Hb E-β-thal patients from various geographic regions or different ethnic groups.

  3. High mobility group B1 levels in sepsis and Disseminated Intravascular Coagulation.

    Science.gov (United States)

    Eskici, Zeynep M; Açıkgöz, Şerefden; Pişkin, Nihal; Mungan, Görkem; Can, Murat; Güven, Berrak; Köktürk, Fürüzan

    2012-01-01

    Cytokines trigger coagulant and fibrinolytic systems in sepsis to result in Disseminated Intravascular Coagulation (DIC) that is an important complication and leads to disseminated hemorrhages and multi-organ failure. High Mobility Group B1 DNA Binding (HMGB1) protein is a cytokine taking part in systemic inflammatory response. The objective of this study was to investigate HMGB1 levels in groups of septic patients with and without DIC.Twenty-one septic patients without DIC and 12 septic patients with DIC from the Intensive Care Unit (ICU) were included in the study. In addition, 20 patients admitted to the ICU without sepsis or DIC and 20 healthy volunteers served as controls. Levels of HMGB1, prothrombin time, activated partial thromboplastin time, fibrinogen, D-dimer, protein C, protein S, anti-thrombin III (ATIII), platelet (thrombocyte) and leukocyte count were determined. Levels of fibrinogen, protein C, ATIII and platelet count were significantly lower and D-dimer was significantly higher in the group with sepsis plus DIC compared to the group with sepsis without DIC. Levels of HMGB1 were higher in the group with sepsis and DIC compared to the group with sepsis; however, the difference was not statistically significant and the levels of HGMB1 of both groups were significantly higher compared to ICU and healthy control groups. HMGB1 levels were not significantly different in survivor and non survivor patients. HMGB1 levels did not differ in lower respiratory tract infection (LRTI) and urinary tract infection (UTI) in regard to the etiology of sepsis.

  4. Relationship between HbA1c and cardiovascular disease risk factors and morbidity of metabolic syndrome in obese children%肥胖儿童糖化血红蛋白与心血管危险因素和代谢综合征发病的关系

    Institute of Scientific and Technical Information of China (English)

    李岩; 刘戈力; 杨箐岩; 郑荣秀; 鲍鹏丽; 高芳芳; 李亚璞; 王燕利

    2013-01-01

    目的 探讨肥胖儿童糖化血红蛋白(HbA1c)水平与心血管疾病危险因素和代谢综合征(MS)发病的关系.方法 根据HbA1c水平将209例肥胖儿童分为I组(HbA1c≥5.7%)122例,Ⅱ组(HbA1c<5.7%)87例;选取89名正常体质量儿童为Ⅲ组(对照组).比较三组儿童的各项代谢和心血管指标及两组肥胖儿童间MS检出率.观察HbA1c水平与各心血管疾病危险因素的相关程度.采用受试者工作特征曲线(ROC)观察以HbA1c为筛查标准诊断MS的最佳切点.结果 Ⅰ组体质指数(BMI)、腰围(WC)高于Ⅱ组、Ⅲ组,高密度脂蛋白胆固醇(HDL-C)浓度低于Ⅱ组、Ⅲ组,差异均有统计学意义(P均<0.05);I组空腹胰岛素(HNS)、口服糖耐量试验(OGTT)0.5 h及1 h血糖浓度、胰岛素抵抗指数(HOMA-IR)高于Ⅱ组,胰岛素敏感指数(HOMA-IS)低于Ⅱ组,差异有统计学意义(P均<0.05).I组收缩压(SBP)、舒张压(DBP)、胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)浓度高于Ⅲ组,差异均有统计学意义(P均<0.01).Ⅱ组BMI、WC、SBP、DBP、TC和LDL-C浓度高于Ⅲ组,HDL-C浓度低于Ⅲ组,差异均有统计学意义(P均<0.01).Spearman相关分析提示,HbA1c水平与BMI、WC、FINS、FPG、OGTT 0.5 h、1 h、2 h血糖、TC、LDL-C、SBP、DBP、HOMA-IR呈正相关;与HDL-C水平呈负相关.Ⅰ组、Ⅱ组检出MS分别为47.54%、29.88%,差异有统计学意义(P=0.010).HbA1c≥5.7作为筛查标准诊断MS的敏感性为56.71%,特异性为76.44%,ROC曲线下面积为0.69(95%CI 0.61~0.76,P<0.000).结论 肥胖患儿HbA1c水平与心血管危险因素密切相关,需注意HbA1c监测调控.%Objective To study the relationship between HbAlc and cardiovascular disease (CVD) risk factors and morbidity of metabolic syndrome (MS) in obese children. Methods According to the HbAlc level, 209 obese children were divided into two groups: group Ⅰ (n=122, HbAlc≥5.7) and group Ⅱ (n=87, HbAlc<5.7%). 89 normal weight subjects were selected as group

  5. The far-reaching effects of power: at the individual, dyadic, and group levels

    NARCIS (Netherlands)

    Galinsky, A.D.; Chou, E.Y.; Halevy, N.; Van Kleef, G.A.; Neale, M.A.; Mannix, E.A.

    2012-01-01

    Purpose - This chapter provides a framework that captures the fundamental impacts of power at the individual, dyadic, small group, and organizational levels. Within each level, we trace the psychological, cognitive, and behavioral consequences of having or lacking power. Approach - We integrate theo

  6. 糖尿病腹膜透析患者血清糖化血红蛋白水平与颈动脉钙化及心血管事件的关系%Associations among HbA1C, carotid plaques and cardiovascular events in peritoneal dialysis patients with diabetes mellitus

    Institute of Scientific and Technical Information of China (English)

    江丽屏; 梁剑波; 王泽彬; 詹俊琳

    2014-01-01

    Objective To investigate the correlations among HbA 1C , carotid plaques and cardiovascular events in peritoneal dialysis patients with diabetes mellitus .Methods Sixty peritoneal dialysis patients , whose original diseases were diabetes mellitus, were divided into two groups according to the serum HbA 1C level.HbA1C and other serum related parameters were assessed , and carotid intima-media thickness ( cIMT) was checked by B-mode ultrasonography .After one year follow-up, the associations among the serum HbA 1C level, deterioration of cIMT and newly -occurring cardio-vascular events were analyzed .Results Significantly higher cIMT and cardiovascular event rate were observed in high HbA1C group than low HbA1C group (P<0.05).After one year follow-up, progression of cIMT and newly occurring car-diovascular events were significantly higher in high HbA 1C group than low HbA1C group.Logistic regression analysis indica-ted that serum HbA1C level was risk factor of cIMT (OR=1.72, 95%CI:0.92~3.87, P=0.024);while serum HbA1C (OR=1.43, 95%CI:0.73~3.46, P=0.012) level and cIMT (OR=3.17, 95%CI:1.38~5.99, P=0.018) were risk factors of cardiovascular events in peritoneal dialysis patients with diabetes mellitus .Conclusion Serum HbA1C is a risk factor of carotid plaques , and serum HbA1C and cIMT were risk factors of cardiovascular events in peritoneal dialysis with diabetes mellitus .%目的:探讨糖尿病腹膜透析患者糖化血红蛋白( HbA1C )水平与颈动脉钙化及心血管事件的关系。方法将60例基础病为糖尿病肾病的腹膜透析患者按照HbA1C的水平分成两组,检测血清HbA1C水平及相关因素,所有的患者均接受颈动脉斑块超声检测,随访1年,观察两组患者血清HbA1C水平、颈动脉钙化的动态变化及心血管事件发生情况。结果低HbA1C组患者颈动脉钙化及心血管事件发生均低于高HbA1C组( P<0.05);随访1年,低HbA1C组的颈动脉钙化进展及新发心血管事件明显少于高Hb

  7. Factors associated with reaching or not reaching target HbA1c after initiation of basal or premixed insulin in patients with type 2 diabetes.

    Science.gov (United States)

    Scheen, A J; Schmitt, H; Jiang, H H; Ivanyi, T

    2017-02-01

    To evaluate factors associated with reaching or not reaching target glycated haemoglobin (HbA1c) levels by analysing the respective contributions of fasting hyperglycaemia (FHG), also referred to as basal hyperglycaemia, vs postprandial hyperglycaemia (PHG) before and after initiation of a basal or premixed insulin regimen in patients with type 2 diabetes. This post-hoc analysis of insulin-naïve patients in the DURABLE study randomised to receive either insulin glargine or insulin lispro mix 25 evaluated the percentages of patients achieving a target HbA1c of reached the target HbA1c. The higher the HbA1c quartile, the greater was the decrease in HbA1c, but also the smaller the percentage of patients achieving the target HbA1c. HbA1c and FHG decreased more in patients reaching the target, resulting in significantly lower values at endpoint in all baseline HbA1c quartiles with either insulin treatment. Patients not achieving the target HbA1c had slightly higher insulin doses, but lower total hypoglycaemia rates. Smaller decreases in FHG were associated with not reaching the target HbA1c, suggesting a need to increase basal or premixed insulin doses to achieve targeted fasting plasma glucose and improve patient response before introducing more intensive prandial insulin regimens. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  8. KADAR HbA1c DAN RASIO LIPID PADA WANITA DEWASA DENGAN OBESITAS SENTRAL

    Directory of Open Access Journals (Sweden)

    Lisa Sudaryanto

    2016-06-01

    Full Text Available Central obesity was accumulation of fat in the abdominal region. Many studies showed correlations between central obesity and cardiovascular diseases, e.g. diabetes and dyslipidemia.  This study was conducted to know the difference between HbA1c and lipid profil between the women with and without central obesity. This study was an analytic observational study with cross-sectional design. Subjects of 52 respondents were healthy adult women staff in campus I, II, III Sanata Dharma University in Yogyakarta and selected using purposive sampling technique. The data of waist circumference, pelvic/hip circumference, HbA1c and lipid profile were collected among the subjects and analyzed with computer with 95% confidence interval. The results of this study showed HbA1c levels and lipid profile were different between the women with and without central obesity, although the difference was not statistically significant.

  9. Role of HbA1c in the Screening of Diabetes Mellitus in a Korean Rural Community

    Directory of Open Access Journals (Sweden)

    Jae Hyun Kim

    2012-02-01

    Full Text Available BackgroundRecently, the measurement of glycated hemoglobin (HbA1c was recommended as an alternative to fasting plasma glucose or oral glucose tolerance tests for diagnosing diabetes mellitus (DM. In this study, we analyzed HbA1c levels for diabetes mellitus screening in a Korean rural population.MethodsWe analyzed data from 10,111 subjects from a Korean Rural Genomic Cohort study and generated a receiver operating characteristic curve to determine an appropriate HbA1c cutoff value for diabetes.ResultsThe mean age of the subjects was 56.3±8.1 years. Fasting plasma glucose and 2-hour plasma glucose after 75 g oral glucose tolerance tests were 97.5±25.6 and 138.3±67.1 mg/dL, respectively. The mean HbA1c level of the subjects was 5.7±0.9%. There were 8,809 non-DM patients (87.1% and 1,302 DM patients (12.9%. A positive relationship between HbA1c and plasma glucose levels and between HbA1c and 2-hour plasma glucose levels after oral glucose tolerance tests was found in a scatter plot of the data. Using Youden's index, the proper cutoff level of HbA1c for diabetes mellitus screening was 5.95% (sensitivity, 77%; specificity, 89.4%.ConclusionOur results suggest that the optimal HbA1c level for DM screening is 5.95%.

  10. Diabetes Support Groups Improve Patient’s Compliance and Control Blood Glucose Levels

    Directory of Open Access Journals (Sweden)

    Zamrotul Izzah

    2013-09-01

    Full Text Available Providing information is not enough to improve diabetic patient’s compliance and achieve goals of therapy. Patient’s good awareness as well as emotional and social supports from family and community may play an important role to improve their compliance and clinical outcomes. Therefore, diabetes support groups were developed and each support group consisted of two pharmacists, two nurses, diabetic patients and their family members. A total of 70 type 2 diabetic patient’s were enrolled and randomized into support group 1 and support group 2. Patients in the group 1 received information leaflets only, while patient in the group 2 received pharmacist counselling and information leaflets at each meeting. Patient’s awareness of diabetes and compliance with medications were assessed by a short questionnaire at baseline and final follow-up. Blood glucose and cholesterol levels were also evaluated in both groups. At the end of study, the overall patient’s awareness and compliance improved by 61.5%. The random and fasting blood glucose levels decreased over than 30% in the group 2 and around 14% in the group 1. This study reveals that collaboration between health care professionals and community in the diabetes support group might help diabetic patients to increase their knowledge and compliance with the diabetes therapy as well as glycaemic control.

  11. Plasma microRNA-451 as a novel hemolytic marker for β0-thalassemia/HbE disease

    Science.gov (United States)

    Leecharoenkiat, Kamonlak; Tanaka, Yuka; Harada, Yasuko; Chaichompoo, Porntip; Sarakul, Orawan; Abe, Yasunobu; Smith, Duncan Richard; Fucharoen, Suthat; Svasti, Saovaros; Umemura, Tsukuru

    2017-01-01

    In Southeast Asia, particularly in Thailand, β0-thalassemia/hemoglobin E (HbE) disease is a common hereditary hematological disease. It is associated with pathophysiological processes, such as the intramedullary destruction of immature erythroid cells and peripheral hemolysis of mature red blood cells. MicroRNA (miR) sequences, which are short non-coding RNA that regulate gene expression in a suppressive manner, serve a crucial role in human erythropoiesis. In the present study, the plasma levels of the erythroid-expressed miRNAs, miR-451 and miR-155, were analyzed in 23 patients with β0-thalassemia/HbE and 16 control subjects. Reverse transcription-quantitative polymerase chain reaction analysis revealed significantly higher levels of plasma miR-451 and miR-155 in β0-thalassemia/HbE patients when compared to the control subjects. Notably, among the β0-thalassemia/HbE patients, a significant increase in miR-451 levels was detected in severe cases when compared with mild cases. The levels of plasma miR-451 correlated with reticulocyte and platelet counts. The results suggest that increased plasma miR-451 levels may be associated with the degree of hemolysis and accelerated erythropoiesis in β0-thalassemia/HbE patients. In conclusion, miR-451 may represent a relevant biomarker for pathological erythropoiesis associated with β0-thalassemia/HbE. PMID:28447765

  12. Leptin levels distribution and ethnic background in two populations from Chile: Caucasian and Mapuche groups.

    Science.gov (United States)

    Pérez-Bravo, F; Albala, C; Santos, J L; Yañez, M; Carrasco, E

    1998-10-01

    Leptin, the product of the human ob gene is increased in obese individuals, suggesting resistance to its effect. We examined the relationship of serum leptin levels with respect to obesity, gender and insulin levels in two populations with different ethnic compositions in Chile. Leptin and insulin levels were determined by radioimmunoassay (RIA) and correlated with body mass index (BMI), gender and ethnic background. 79 Caucasian subjects from Santiago and 65 Mapuche natives from the Araucania region, Chile, were included in this study. Leptin concentrations in obese subjects were significantly increased in both ethnic groups in relation to lean status: Caucasian and Mapuche obese 19.3 +/- 11.6 and 10.1 +/- 5.8 (P Mapuche lean 10.4 +/- 5.8 and 4.7 +/- 2.9 (P Mapuche and Caucasian groups, similar leptin levels were observed among the males of the two populations in both metabolic states (lean and obese). In contrast, the leptin level distributions between women showed a marked difference, having a minor value in the Mapuche women with a comparable value with the male group in this ethnic population. The leptin concentrations are associated with obesity in both ethnic groups in Chile. However, the leptin levels between the Mapuche natives were significantly decreased compared to the Caucasian group. The gender distribution does not seem to be important in the Mapuche natives. The ethnic composition seems to be important in the leptin distribution in the analysed populations.

  13. [The effect of group-based psychodrama therapy on decreasing the level of aggression in adolescents].

    Science.gov (United States)

    Karataş, Zeynep; Gökçakan, Dan Zafer

    2009-01-01

    This study aimed to examine the effect of group-based psychodrama therapy on the level aggression in adolescents. The study included 23 students from Nezihe Yalvac Anatolian Vocational High School of Hotel Management and Tourism that had high aggression scores. Eleven of the participants (6 female, 5 male) constituted the experimental group and 12 (6 male, 6 female) were in the control group. The 34-item Aggression Scale was used to measure level of aggression. We utilized mixed pattern design including experiment-control, pre-test and post test and follow up. The experimental group participated in group-based psychodrama therapy once a week for 90 minutes, for 14 weeks in total. The Aggression Scale was administered to the experimental and control groups before and after treatment; it was additionally administered to the experimental group 16 weeks after treatment. Data were analyzed using ANCOVA and dependent samples t tests. Our analysis shows that group-based psychodrama had an effect on the experimental group in terms of total aggression, anger, hostility, and indirect aggression scores (F=65.109, F=20.175, F=18.593, F=40.987, respectively, P<.001). There was no effect of the group-based treatment on verbal or physical aggression scores. Follow-up indicated that the effect of the therapy was still measureable 16 weeks after the cessation of the therapy. Results of the present study indicate that group-based psychodrama therapy decreased the level of aggression in the experimental group. Current findings are discussed with reference to the literature. Recommendations for further research and for psychiatric counselors are provided.

  14. P(3HB-co-4HB)生物降解性能的研究%Study on the Biodegradation Performance of P(3HB-co-4HB)

    Institute of Scientific and Technical Information of China (English)

    张竞; 温幸; 王晓芳; 史立林

    2014-01-01

    本实验对5种4HB摩尔含量的P(3HB-co-4HB)在含有脂肪酶的磷酸缓冲液中的生物降解性能进行了研究,分析了降解机理和降解影响因素.通过质量变化、分子量变化、膜表面形态表征了其生物降解性能.结果表明,4HB含量对脂肪酶降解过程的影响较小;酶解过程中5种P(3HB-co-4HB)质量损失速率和分子量降低程度相差不大;P(3HB-co-15%4HB)具有较低的结晶度和较好的表面粗糙度,因此降解速率最快.SEM图像表明脂肪酶对于P(3HB-co-4HB)具有较快的降解能力,其酶降解过程是一个表面腐蚀的过程.

  15. A novel hemoglobin variant found on the α1 chain: Hb KSVGH (HBA1: p.Lys57_Gly58insSerHisGlySerAlaGlnValLys).

    Science.gov (United States)

    Wang, Mei-Chun; Tsai, Kuo-Wang; Chu, Chih-Hsun; Yu, Ming-Sun; Lam, Hing-Chung

    2015-01-01

    Glycosylated hemoglobin (Hb A1C) is a crucial indicator for the long-term control and the diagnosis of diabetes. However, the presence of hemoglobin (Hb) variants may affect the measured value of Hb A1C and result in an abnormal graph trend and inconsistency between the clinical blood sugar test and Hb A1C values. In this study, laboratory data of 41,267 patients with diabetes were collected. The Hb A1C levels and the graph results were examined. We identified 74 cases containing abnormal Hb A1C graph trends. The conducted blood cell counts and capillary Hb electrophoresis were used to analyze Hb variants. We also determined gene variation for the Hb variants by a sequence approach. Fifteen different types of Hb variants were identified in this study. Among these, we found a novel variant in which the α1 subunit of Hb showed an insertion of 24 nucleotides (nts) between the 56th and 57th residues. We named this novel variant Hb Kaohsiung Veterans General Hospital (Hb KSVGH) (HBA1: p.Lys57_Gly58insSerHisGlySerAlaGlnValLys).

  16. Deferasirox effectively decreases iron burden in patients with double heterozygous HbS/β-thalassemia.

    Science.gov (United States)

    Voskaridou, Ersi; Plata, Eleni; Douskou, Marousa; Sioni, Anastasia; Mpoutou, Efrosini; Christoulas, Dimitrios; Dimopoulou, Maria; Terpos, Evangelos

    2011-01-01

    Iron overload is present in several cases of double heterozygous sickle-cell/beta-thalassemia (HbS/β-thal). Deferasirox is an orally administered iron chelator which is effective on iron overloaded patients with transfusion-dependent anemia. The aim of this study was to investigate the efficacy and safety of deferasirox on HbS/β-thal patients with iron overload. We evaluated 31 adult patients with HbS/β-thal (14M/17F; median age 41 years) who had serum ferritin levels >1,000 ng/mL and who were sporadically transfused. Total iron burden was monitored by measuring serum ferritin levels before and monthly after starting deferasirox, while liver iron concentration and cardiac iron burden were measured by magnetic resonance imaging (MRI) T2 and T2* parameters at baseline and 12 months after deferasirox treatment. Deferasirox managed to reduce the mean serum ferritin levels after 12 months of treatment from 1,989 ± 923 to 1,008 ± 776 ng/mL (P deferasirox provided effective control of iron levels (mainly of the liver) in minimally transfused patients with HbS/β-thal, without significant adverse events, at similar doses to those studied widely for the treatment of patients with thalassemia syndromes.

  17. To establish trimester-specific reference ranges for glycated haemoglobin (HbA1c) in pregnancy

    LENUS (Irish Health Repository)

    O'Connor, CM

    2011-09-01

    Background and aims: Diabetes in Pregnancy imposes additional risks to both mother and infant. These poor outcomes are considered to be primarily related to glycaemic control which is monitored longitudinally through pregnancy by means of HbA1c. The correlation between HbA1c levels with clinical outcomes emphasises the need to measure HbA1c accurately, precisely and for data interpretation comparison to appropriately defined reference intervals. From July 1st 2010, the HbA1c assay in Irish laboratories became fully metrologically traceable to the IFCC standard, permitting HbA1c to be reported in IFCC units (mmol\\/mol) and derived DCCT\\/NGSP units (%) using the IFCC-DCCT\\/NGSP master equation (DCCT = Diabetes Control and Complications Trial, NGSP = National Glycohemoglobin standardisation program). The aim of this project is to establish trimester-specific reference ranges in pregnancy for IFCC standardised HbA1c in non-diabetic Caucasian women. This will allow us to define the goal for HbA1c during pregnancy complicated by diabetes.\\r\

  18. The Effect of Group Counseling Program on 8th Grade Students’ Assertiveness Levels

    Directory of Open Access Journals (Sweden)

    İsmail SEÇER

    2014-06-01

    Full Text Available This study is conducted to determine the effects of group counseling program on elementary school 8th grade students’ assertiveness skills. Study group was formed of 100 students getting educated in Erzurum Kültür Elementary School in 2011-2012 education years. RAE-Rathus Assertiveness Schedule was applied on this group to gather data. 30 students getting lower scores from the inventory have been randomly divided into experimental and control groups. Each group is formed of 15 students. Group counseling program has been carried out for 8 weeks on the experimental group to improve the students’ assertiveness skills. Single-way and two-way analysis of covariance (ANCOVA has been used in the analysis of the data. The data is analyzed with SPSS 19.00. The results of the study show that assertiveness skills of the students who participate in the group counseling program has increased significantly compared to the control group and to the preexperimental circumstances. Besides, it is determined that the change observed in the experimental group has been occurred separately from the age and socio-economic level variables, and it is determined with the monitoring test applied after four months that this affect is continued. According to this result, it can be said that the applied group counseling program is an effective means to improve the assertiveness skills of elementary school students

  19. Comparison of Calcitonin Gene Related Peptide Level between Children with Dilated Cardiomyopathy and Control Group

    Directory of Open Access Journals (Sweden)

    Noormohammad Noori

    2015-06-01

    Full Text Available Background: Dilated cardiomyopathy is revealed with left ventricular dilatation and systolic dysfunction. Objectives: This study aimed to compare the children with dilated cardiomyopathy and control group regarding the level of Calcitonin Gene Related Peptide (CGRP and its relationship with echocardiography findings Patients and Methods: This case-control study was conducted on 37 children with dilated cardiomyopathy and free of any clinical symptoms and 37 healthy age- and sex-matched children referring to Ali-e-Asghar and Ali Ebne Abitaleb hospitals in Zahedan, Iran. After taking history, echocardiography was performed for both groups. The data were analyzed using the SPSS statistical software and appropriate statistical tests. Results: The two groups were significantly different regarding most of the echocardiographic parameters (P < 0.05. Also, a significant difference was found between the two groups concerning the mean CGRP levels (P = 0.001. Among echocardiographic parameters, CGRP was directly related to Interventricular Septal dimension in Systole (IVSS (P = 0.022, R = 0.375. However, no significant relationship was observed between CGRP level and Ross classification. Conclusions: The findings of this study showed an increase in CGRP serum levels in the case group. Besides, a direct correlation was observed between CGRP level and IVSS.

  20. COMPARISON OF LEVELS OF SELF-ESTEEM BY SEX AND LEVEL OF PHYSICAL ACTIVITY IN TWO GROUPS OF SENIOR ADULTS

    Directory of Open Access Journals (Sweden)

    Diego Rodríguez Méndez

    2016-08-01

    Full Text Available The purpose of this study is to observe whether there are differences in the self-esteem score, depending on the level of physical activity (active-sedentary in a sample of senior adults. Twenty-six senior adults participated in the study with an average of 73 ± 9 years of age. The sedentary group (n = 12 belonged to the Santo Domingo Nursing Home in Heredia, Costa Rica, while the active group belonged to the project entitled Modulation of the Aging Process of the Movement for Life Program. Results: No significant differences were found in scores by level of physical activity (t = 0.931, p = 0.363; however, there were significant differences in self-esteem scores by gender (t = -2.255, p = 0.034. It was concluded that the level of physical activity does not affect self-esteem and that men’s level of self-esteem is higher than women’s.

  1. Arbuscular mycorrhizal fungal phylogenetic groups differ in affecting host plants along heavy metal levels.

    Science.gov (United States)

    He, Lei; Yang, Haishui; Yu, Zhenxing; Tang, Jianjun; Xu, Ligen; Chen, Xin

    2014-10-01

    Arbuscular mycorrhizal fungi (AMF) are important components of soil microbial communities, and play important role in plant growth. However, the effects of AMF phylogenetic groups (Glomeraceae and non-Glomeraceae) on host plant under various heavy metal levels are not clear. Here we conducted a meta-analysis to compare symbiotic relationship between AMF phylogenetic groups (Glomeraceae and non-Glomeraceae) and host plant functional groups (herbs vs. trees, and non-legumes vs. legumes) at three heavy metal levels. In the meta-analysis, we calculate the effect size (ln(RR)) by taking the natural logarithm of the response ratio of inoculated to non-inoculated shoot biomass from each study. We found that the effect size of Glomeraceae increased, but the effect size of non-Glomeraceae decreased under high level of heavy metal compared to low level. According to the effect size, both Glomeraceae and non-Glomeraceae promoted host plant growth, but had different effects under various heavy metal levels. Glomeraceae provided more benefit to host plants than non-Glomeraceae did under heavy metal condition, while non-Glomeraceae provided more benefit to host plants than Glomeraceae did under no heavy metal. AMF phylogenetic groups also differed in promoting plant functional groups under various heavy metal levels. Interacting with Glomeraceae, herbs and legumes grew better than trees and non-legumes did under high heavy metal level, while trees and legumes grew better than herbs and non-legumes did under medium heavy metal level. Interacting with non-Glomeraceae, herbs and legumes grew better than trees and non-legumes did under no heavy metal. We suggested that the combination of legume with Glomeraceae could be a useful way in the remediation of heavy metal polluted environment.

  2. Evaluation of the effect of self-care education based on Vark learning style on HbA1c and FBS in patients with type II diabetes

    Directory of Open Access Journals (Sweden)

    Saleh Moghadam Amir Reza

    2016-08-01

    Full Text Available Background and Objective: Patients with type two diabetes mostly struggle with increased fasting blood sugar (FBS and glycosylated hemoglobin HbA1c, mainly associated with irrecoverable complications. Self-care education and considering different types of learning among patients are regarded as some of the most important issues in this regard. Therefore, this study aimed to evaluate the effect of self-care education based on VARK learning style on HbA1c and FBS in patients with type two diabetes. Materials and Method: This clinical trial was conducted on patients with type two diabetes, referring to Parsian Clinic in Mashhad, Iran in 2015. In total, 72 samples were selected through randomized convenience sampling and divided into two control and intervention groups of 36 cases. Subjects of the intervention group were also divided into subgroups of visual, aural, read/write, and kinesthetic based on the results of VARK inventory. Self-care education was carried for the intervention group in two 60-minute session once every two weeks, tailored to learning styles of patients. Meanwhile, traditional lecture method was used for the control group. HbA1c and FBS were evaluated in all the participants before and a month and a half after the intervention to assess the self-care of patients. Data analysis was performed in SPSS version 21 using Mann-Whitney U, Chi-square, independent t-test and Wilcoxon. Results: In this study, mean score of HbA1c was decreased from 7.7±0.8 to 7.0±5.7 (P<0.062, whereas mean score of FBS was alleviated from 176.1±33.5 to 147.7±32.8 (P<0.001, which was only significant regarding FBS levels (P=0.002. Conclusion: According to the results of this study, application of VARK learning style led to a reduction in HbA1c and FBS levels, contributing to improved self-care in patients with type 2 diabetes. Therefore, before initiation of training programs, determining learning style of patients is suggested using VARK learning

  3. [The value of MCV, MCH and HbA(2) in laboratory screening of thalassemia].

    Science.gov (United States)

    Li, Li-yan; Li, Qiang; Song, Lan-lin; Jin, Wang-jie; Ma, Zhen-hua; Yu, Yan-hong; Zhong, Mei

    2012-02-01

    To explore the roles of mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and hemoglobin A(2) (HbA(2)) in the laboratory screening of thalassemia, and to find optimal screening modality for different conditions. From September 2008 to May 2011, 1384 subjects underwent thalassemia screening at Department of Obstetrics and Gynecology of Nanfang Hospital. Of them, 1036 cases were diagnosed with thalassemia (408 α-thalassemia, 608 β-thalassemia, and 20 αβ compound thalassemia, thalassemia group) and 348 without thalassemia, non-thalassemia group. All subjects were screened respectively for MCV, MCH and HbA(2). Analyses were performed in all subjects to assess the sensitivity, specificity, positive predictive value, negative predictive value and diagnostic accuracy respectively associated with MCV, MCH and HbA(2) alone, combination of MCV and MCH, and combination of MCV, MCH and HbA(2). (1) In the thalassemia group, the sensitivity of MCV alone was 92.9% (379/408) for α thalassemia, 99.3% (604/608) for β thalassemia and 100.0% (20/20) for αβ compound thalassemia. In the non-thalassemia group, the specificity of MCV alone was 75.0% (261/348). (2) In the thalassemia group, the sensitivity of MCH alone was 92.9% (379/408) in α thalassemia, 99.0% (602/608) in β thalassemia and 100.0% (20/20) in αβ compound thalassemia. In the non-thalassemia group, the specificity of MCH alone was 72.7% (253/348). (3) The sensitivity of Hb A(2) alone was 67.4% (275/408) for α thalassemia, 97.5% (593/608) for β thalassemia, and 100% (20/20) for αβ compound thalassemia while it's specificity was 72.4% (252/348) in the non-thalassemia group. (4) With positive indexes of MCV, MCH and MCV + MCH, when HbA(2) > 3.5% it had a high value in β-thalassemia screening, but when HbA(2) screening. (5) As a single marker, MCV and MCH had better sensitivity, specificity, positive predictive value, negative predictive value and diagnosis accuracy than HbA(2). MCV + MCH was the

  4. A Study Assessing the Association of Glycated Hemoglobin A1C (HbA1C) Associated Variants with HbA1C, Chronic Kidney Disease and Diabetic Retinopathy in Populations of Asian Ancestry

    Science.gov (United States)

    Chen, Peng; Ong, Rick Twee-Hee; Tay, Wan-Ting; Sim, Xueling; Ali, Mohammad; Xu, Haiyan; Suo, Chen; Liu, Jianjun; Chia, Kee-Seng; Vithana, Eranga; Young, Terri L.; Aung, Tin; Lim, Wei-Yen; Khor, Chiea-Chuen; Cheng, Ching-Yu; Wong, Tien-Yin; Teo, Yik-Ying; Tai, E-Shyong

    2013-01-01

    Glycated hemoglobin A1C (HbA1C) level is used as a diagnostic marker for diabetes mellitus and a predictor of diabetes associated complications. Genome-wide association studies have identified genetic variants associated with HbA1C level. Most of these studies have been conducted in populations of European ancestry. Here we report the findings from a meta-analysis of genome-wide association studies of HbA1C levels in 6,682 non-diabetic subjects of Chinese, Malay and South Asian ancestries. We also sought to examine the associations between HbA1C associated SNPs and microvascular complications associated with diabetes mellitus, namely chronic kidney disease and retinopathy. A cluster of 6 SNPs on chromosome 17 showed an association with HbA1C which achieved genome-wide significance in the Malays but not in Chinese and Asian Indians. No other variants achieved genome-wide significance in the individual studies or in the meta-analysis. When we investigated the reproducibility of the findings that emerged from the European studies, six loci out of fifteen were found to be associated with HbA1C with effect sizes similar to those reported in the populations of European ancestry and P-value ≤ 0.05. No convincing associations with chronic kidney disease and retinopathy were identified in this study. PMID:24244560

  5. GMove: Group-Level Mobility Modeling Using Geo-Tagged Social Media

    Science.gov (United States)

    Zhang, Chao; Zhang, Keyang; Yuan, Quan; Zhang, Luming; Hanratty, Tim; Han, Jiawei

    2017-01-01

    Understanding human mobility is of great importance to various applications, such as urban planning, traffic scheduling, and location prediction. While there has been fruitful research on modeling human mobility using tracking data (e.g., GPS traces), the recent growth of geo-tagged social media (GeoSM) brings new opportunities to this task because of its sheer size and multi-dimensional nature. Nevertheless, how to obtain quality mobility models from the highly sparse and complex GeoSM data remains a challenge that cannot be readily addressed by existing techniques. We propose GMove, a group-level mobility modeling method using GeoSM data. Our insight is that the GeoSM data usually contains multiple user groups, where the users within the same group share significant movement regularity. Meanwhile, user grouping and mobility modeling are two intertwined tasks: (1) better user grouping offers better within-group data consistency and thus leads to more reliable mobility models; and (2) better mobility models serve as useful guidance that helps infer the group a user belongs to. GMove thus alternates between user grouping and mobility modeling, and generates an ensemble of Hidden Markov Models (HMMs) to characterize group-level movement regularity. Furthermore, to reduce text sparsity of GeoSM data, GMove also features a text augmenter. The augmenter computes keyword correlations by examining their spatiotemporal distributions. With such correlations as auxiliary knowledge, it performs sampling-based augmentation to alleviate text sparsity and produce high-quality HMMs. Our extensive experiments on two real-life data sets demonstrate that GMove can effectively generate meaningful group-level mobility models. Moreover, with context-aware location prediction as an example application, we find that GMove significantly outperforms baseline mobility models in terms of prediction accuracy.

  6. GMove: Group-Level Mobility Modeling Using Geo-Tagged Social Media.

    Science.gov (United States)

    Zhang, Chao; Zhang, Keyang; Yuan, Quan; Zhang, Luming; Hanratty, Tim; Han, Jiawei

    2016-08-01

    Understanding human mobility is of great importance to various applications, such as urban planning, traffic scheduling, and location prediction. While there has been fruitful research on modeling human mobility using tracking data (e.g., GPS traces), the recent growth of geo-tagged social media (GeoSM) brings new opportunities to this task because of its sheer size and multi-dimensional nature. Nevertheless, how to obtain quality mobility models from the highly sparse and complex GeoSM data remains a challenge that cannot be readily addressed by existing techniques. We propose GMove, a group-level mobility modeling method using GeoSM data. Our insight is that the GeoSM data usually contains multiple user groups, where the users within the same group share significant movement regularity. Meanwhile, user grouping and mobility modeling are two intertwined tasks: (1) better user grouping offers better within-group data consistency and thus leads to more reliable mobility models; and (2) better mobility models serve as useful guidance that helps infer the group a user belongs to. GMove thus alternates between user grouping and mobility modeling, and generates an ensemble of Hidden Markov Models (HMMs) to characterize group-level movement regularity. Furthermore, to reduce text sparsity of GeoSM data, GMove also features a text augmenter. The augmenter computes keyword correlations by examining their spatiotemporal distributions. With such correlations as auxiliary knowledge, it performs sampling-based augmentation to alleviate text sparsity and produce high-quality HMMs. Our extensive experiments on two real-life data sets demonstrate that GMove can effectively generate meaningful group-level mobility models. Moreover, with context-aware location prediction as an example application, we find that GMove significantly outperforms baseline mobility models in terms of prediction accuracy.

  7. Hemoglobin A1c Level Is Not Related to the Severity of Atherosclerosis in Patients with Acute Coronary Syndrome

    Directory of Open Access Journals (Sweden)

    Xinhong Wang

    2015-01-01

    Full Text Available Background. The relationship between hemoglobin A1c (HbA1c levels and the extent of coronary artery stenosis in patients with acute coronary syndrome (ACS remains uncertain. The present study aimed to assess the correlation of HbA1c level with angiographic coronary atherosclerosis. Methods. 292 consecutive ACS patients were enrolled and stratified into three groups according to HbA1c levels (group 1: 40 by logistic regression analysis. Diabetes mellitus (DM and LVEF levels were two independent risk factors for high Gensini score. Conclusions. HbA1c level is not a significant and independent marker for the severity of angiography in ACS patients, even in high-risk patients.

  8. Brassica napus L. Homeodomain Leucine-Zipper Gene BnHB6 Responds to Abiotic and Biotic Stresses

    Institute of Scientific and Technical Information of China (English)

    Shun-Wu YU; Li-Da ZHANG; Kai-Jing ZUO; Dong-Qin TANG; Xiao-Fen SUN; Ke-Xuan TANG

    2005-01-01

    Ahomeodomain leucine-zipper(HD-Zip) gene BnHB6 (GenBank accession No. AY336103) was isolated from oilseed rape (Brassica napus L.) following drought treatment through rapid amplification of cDNA ends (RACE). The full-length cDNA of BnHB6 was 1 611 bp and contained a 936-bp open reading frame encoding 311 amino acids. Sequence analysis indicated that BnHB6 belonged to the HD-Zip I subfamily.High-stringency Southern boltting analysis showed that BnHB6 appeared in rape as a single copy but had homologous genes. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that BnHB6 was expressed in several tissues tested under control conditions, but that expression was significantly upregulated in shoots by mannitol, NaCl, cold treatment, anaerobic culture, wounding, H2O2, abscisic acid (ABA), and salicylic acid (SA) treatments, but not by ultraviolet treatment. Further RTPCR analysis revealed that BnHB6 was a late-responsive gene, the expression of which was not activated by NaCl, cold treatment, H2O2, ABA, and SA at an early time point (20 min) of treatment in the shoot. However, after a certain period of treatment, the induced expression culminated and then declined until the next peak occurred. Tissue-specific analysis revealed that BnHB6 was expressed at certain levels in the roots, shoots, and flowers, and the roots were found to respond to the osmotic stimuli more rapidly than shoots to increase the expression of BnHB6. The present study implies that BnHB6 plays a positive role as a regulator of biotic and abiotic stresses on growth during seedling establishment.

  9. Improving Parental Stress Levels Among Mothers Living with HIV: A Randomized Control Group Intervention Study

    OpenAIRE

    Johnson, Erica R.; Davies, Susan L.; Aban, Inmaculada; Mugavero, Michael J.; Shrestha, Sadeep; Kempf, Mirjam-Colette

    2015-01-01

    Limited knowledge exists regarding parenting efficacy interventions for mothers living with HIV (MLH). This study evaluated the impact of a supportive group intervention on lowering parenting stress among MLH. Eighty MLH were randomized to a parenting (N=34) or health focused (control) (N=46) group intervention. Pre- and post-intervention stress levels were assessed using the Parental Stress Index-Short Form (PSI/SF). Differences in PSI/SF scores were examined using ANOVA, and predictors of P...

  10. A Novel Bit-level Image Encryption Method Based on Chaotic Map and Dynamic Grouping

    Institute of Scientific and Technical Information of China (English)

    张国基; 沈彦

    2012-01-01

    In this paper,a novel bit-level image encryption method based on dynamic grouping is proposed.In the proposed method,the plain-image is divided into several groups randomly,then permutation-diffusion process on bit level is carried out.The keystream generated by logistic map is related to the plain-image,which confuses the relationship between the plain-image and the cipher-image.The computer simulation results of statistical analysis,information entropy analysis and sensitivity analysis show that the proposed encryption method is secure and reliable enough to be used for communication application.

  11. Association of glycosylated hemoglobin level with lipid ratio and individual lipids in type 2 diabetic patients

    Institute of Scientific and Technical Information of China (English)

    Zhe Yan; Yang Liu; Hui Huang

    2012-01-01

    Objective:To study the correlation of lipid ratios and individual lipid indexes of patients with type2 diabetes with glycosylated hemoglobin (HbA1c).Methods:Samples were collected from 128 type2 diabetic patients (aged19-90 years; male 72, female56). The sera were analyzed for HbA1c, total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C). According to theHbA1c level, the patients were divided into three groups, groupA (HbA1c 10%,n=49). The correlation ofHbA1cwith lipid ratios & individual lipid indexes were analyzed.Results:With the increased level ofHbA1c,LDL-C had a significantly increasing trend (P<0.05); whereasTC went up with the increasedHbA1c, without any significant differences between three groups. There was no significant correlation betweenHbA1c andTG or HDL-C. With the increased level ofHbA1c,TC/HDL-C,LDL-C/HDL-C ratios were gradually increased, with significant differences among groups (P<0.05). The lipid ratios, especiallyLDL-C/HDL-C ratio was more susceptible to impaired lipid metabolism inT2DM patients than individual lipid. Conclusions:LDL-C/HDL-C ratio is helpful in assessing and reducing the risk of cardiovascular disease caused by impaired lipid metabolism in type2 diabetic patients.

  12. Salivary TNFα levels in groups of subjects with rheumatoid arthritis and chronic periodontitis.

    Science.gov (United States)

    Gamel, Ehsan B; Hashim, Nada T; Satti, Asim; Gismalla, Bakri G

    2017-01-07

    Rheumatoid arthritis (RA) and chronic periodontitis are the most common chronic inflammatory diseases with significant pathological and clinical similarities. Numerous studies have indicated a relationship between rheumatoid arthritis and periodontal disease. The aim of this study was to compare the TNF-α levels in saliva among patients with Rheumatoid arthritis (RA) and chronic periodontitis as well as healthy subjects. One hundred and seventy-one patients were enrolled in this cross-sectional study. Fifty-seven patients diagnosed of RA, 57 patients with chronic periodontitis and 57 healthy subjects. These patients have been examined with regard to TNF-α level from salivary samples. Their teeth were examined with regard to Plaque Index , Gingival Index, probing depth and clinical attachment level.All patients were non-smokers. The results revealed a significant difference in all periodontal parameters among the three groups. The chronic periodontitis group showed a significantly higher value in all clinical periodontal parameters in comparison to both the RA and healthy groups. No significant difference was found between salivary TNF-α level among the three study groups. Patients with chronic periodontitis had the highest periodontal indices. However there was no significant difference regarding the level of salivary TNF-α. Hence, suppression of proinflammatory cytokines might prove beneficial in suppressing periodontal diseases among RA patients.

  13. Common marmosets show social plasticity and group-level similarity in personality.

    Science.gov (United States)

    Koski, Sonja E; Burkart, Judith M

    2015-03-06

    The social environment influences animal personality on evolutionary and immediate time scales. However, studies of animal personality rarely assess the effects of the social environment, particularly in species that live in stable groups with individualized relationships. We assessed personality experimentally in 17 individuals of the common marmoset, living in four groups. We found their personality to be considerably modified by the social environment. Marmosets exhibited relatively high plasticity in their behaviour, and showed 'group-personality', i.e. group-level similarity in the personality traits. In exploratory behaviour this was maintained only in the social environment but not when individuals were tested alone, suggesting that exploration tendency is subjected to social facilitation. Boldness, in contrast, showed higher consistency across the social and solitary conditions, and the group-level similarity in trait scores was sustained also outside of the immediate social environment. The 'group-personality' was not due to genetic relatedness, supporting that it was produced by social effects. We hypothesize that 'group-personality' may be adaptive for highly cooperative animals through facilitating cooperation among individuals with similar behavioural tendency.

  14. HbA1c levels in non-diabetic older adults - No J-shaped associations with primary cardiovascular events, cardiovascular and all-cause mortality after adjustment for confounders in a meta-analysis of individual participant data from six cohort studies

    OpenAIRE

    Schöttker, Ben; Rathmann, W.; Herder, C.; Thorand, B; Wilsgaard, T; Njølstad, I.; Siganos, G; Mathiesen, E.B.; Saum, K.U.; Peasey, A; Feskens, E.; Boffetta, P.; Trichopoulou, A; Kuulasmaa, K; Kee, F.

    2016-01-01

    Background: To determine the shape of the associations of HbA1c with mortality and cardiovascular outcomes in non-diabetic individuals and explore potential explanations. Methods: The associations of HbA1c with all-cause mortality, cardiovascular mortality and primary cardiovascular events (myocardial infarction or stroke) were assessed in non-diabetic subjects ≥50 years from six population-based cohort studies from Europe and the USA and meta-analyzed. Very low, low, intermediate and increas...

  15. HbA1c levels in non-diabetic older adults – No J-shaped associations with primary cardiovascular events, cardiovascular and all-cause mortality after adjustment for confounders in a meta-analysis of individual participant data from six cohort studies

    OpenAIRE

    Schöttker, Ben; Rathmann, Wolfgang; Herder, Christian; Thorand, Barbara; Wilsgaard, Tom; Njølstad, Inger; Siganos, Galatios; Mathiesen, Ellisiv B.; Saum, Kai-Uwe; Peasey, Anne; Edith J. M. Feskens; Boffetta, Paolo; Trichopoulou, Antonia; Kuulasmaa, Kari; Kee, Frank

    2016-01-01

    Source: doi: 10.1186/s12916-016-0570-1 Background:To determine the shape of the associations of HbA1c with mortality and cardiovascular outcomes in non-diabetic individuals and explore potential explanations. Methods: The associations of HbA1c with all-cause mortality, cardiovascular mortality and primary cardiovascular events (myocardial infarction or stroke) were assessed in non-diabetic subjects ≥50 years from six population-based cohort studies from Europe and the USA and m...

  16. Influence of glycemic control on the levels of subgingival periodontal pathogens in patients with generalized chronic periodontitis and type 2 diabetes

    Science.gov (United States)

    MIRANDA, Tamires Szeremeske; FERES, Magda; RETAMAL-VALDÉS, Belén; PEREZ-CHAPARRO, Paula Juliana; MACIEL, Suellen Silva; DUARTE, Poliana Mendes

    2017-01-01

    Abstract Objective This study evaluated the influence of glycemic control on the levels and frequency of subgingival periodontal pathogens in patients with type 2 diabetes mellitus (DM) and generalized chronic periodontitis (ChP). Material and Methods Fifty-six patients with generalized ChP and type 2 DM were assigned according to the levels of glycated hemoglobin (HbA1c) into one of the following groups: HbA1cTannerella forsythia, Treponema denticola, Eubacterium nodatum, Parvimona micra, Fusobacterium nucleatum ssp. and Prevotella intermedia. Results The mean counts of F. nucleatum ssp. were statistically significantly higher in the sites with PD≥5 mm of the HbA1c≥8% group (p<0.05). Frequencies of detection of T. forsythia, E. nodatum, P. micra and F. nucleatum ssp. were all higher in the sites with PD≥5 mm of the patients with HbA1c≥8%, compared with those of patients with HbA1c<8% (p<0.05). Frequency of detection of P. intermedia was higher in the sites with PD<5 mm of the patients with HbA1c≥8% than those of the patients with HbA1c<8% (p<0.05). Conclusions Poor glycemic control, as indicated by HbA1c≥8%, is associated with increased levels and frequencies of periodontal pathogens in the subgingival biofilm of subjects with type 2 DM and ChP. PMID:28198980

  17. Investigating the Group-Level Impact of Advanced Dual-Echo fMRI Combinations

    Directory of Open Access Journals (Sweden)

    Adam Kettinger

    2016-12-01

    Full Text Available Multi-echo fMRI data acquisition has been widely investigated and suggested to optimize sensitivity for detecting the BOLD signal. Several methods have also been proposed for the combination of data with different echo times. The aim of the present study was to investigate how these advance echo combination methods provide advantages over the simple averaging of echoes when state-of-the-art group-level random-effect analyses are performed. Both resting-state and task-based dual-echo fMRI data were collected from 27 healthy adult individuals (14 male, mean age = 25.75 years using standard echo-planar acquisition methods at 3T. Both resting-state and task-based data were subjected to a standard image pre-processing pipeline. Subsequently the two echoes were combined as a weighted average, using four different strategies for calculating the weights: (1 simple arithmetic averaging, (2 BOLD sensitivity weighting, (3 temporal-signal-to-noise ratio weighting and (4 temporal BOLD sensitivity weighting. Our results clearly show that the simple averaging of data with the different echoes is sufficient. Advanced echo combination methods may provide advantages on a single-subject level but when considering random-effects group level statistics they provide no benefit regarding sensitivity (i.e. group-level t-values compared to the simple echo-averaging approach. One possible reason for the lack of clear advantages may be that apart from increasing the average BOLD sensitivity at the single-subject level, the advanced weighted averaging methods also inflate the inter-subject variance. As the echo combination methods provide very similar results, the recommendation is to choose between them depending on the availability of time for collecting additional resting-state data or whether subject-level or group-level analyses are planned.

  18. Electromagnetically induced transparency and controllable group velocity in a five-level atomic system

    Institute of Scientific and Technical Information of China (English)

    Lihui Jin; Shangqing Gong; Yueping Niu; Shiqi Jin

    2006-01-01

    @@ The optical properties of a five-level atomic system composed of a A-type four-level atomic and a tripod four-level atomic systems are investigated. It is found that the behaviors of electromagnetically induced transparency (EIT) and group velocity can be controlled by choosing appropriate parameters with the interacting dark resonances. In particular, when all the fields are on resonance, the slow light at the symmetric transparency windows with a much broader EIT width is obtained by tuning the intensity of the coupling field in comparison with its sub-system, which provides potential applications in quantum storage and retrieval of light.

  19. The relationship between personality traits and anxiety/depression levels in different drug abusers' groups.

    Science.gov (United States)

    Tatalović Vorkapić, Sanja; Dadić-Hero, Elizabeta; Ružić, Klementina

    2013-01-01

    Aim. Since psychosocial characteristics of drug abuse involve mainly specific personality and emotional changes, it is very important to investigate characteristics of addictive personality in relationship with emotional state of the individual. Considering that, the objective of this study was to analyse the relationship between personality structure and emotional state of two different groups: heroin addicts and recreate drug abusers. Methods. The total of 288 (219 males and 69 females; 191 heroin addicts and 97 recreate drug users) clients of Centre for the prevention and treatment of drug abuse in Rijeka completed Eysenck's Personality Questionnaire (EPQ R/A), Beck's Anxiety Inventory (BAI) and Beck's Depression Inventory (BDI). Their average age was 22. Results. In the group of heroin addicts, higher levels of anxiety and depression were significantly correlated with higher levels of psychoticism, neuroticism, criminality and addiction. In the group of recreate drug users, higher extraversion and social conformity were determined. Furthermore, in the first group was found even higher depression. However when the anxiety level was compared between these two groups, there was no significant difference. Conclusion. Overall, the findings implied that the used measurement instruments could serve as the useful diagnostic tools that could ensure advantageous treatment directions.

  20. The relationship between personality traits and anxiety/depression levels in different drug abusers' groups

    Directory of Open Access Journals (Sweden)

    Tatalović Vorkapić Sanja

    2013-12-01

    Full Text Available AIM: Since psychosocial characteristics of drug abuse involve mainly specific personality and emotional changes, it is very important to investigate characteristics of addictive personality in relationship with emotional state of the individual. Considering that, the objective of this study was to analyse the relationship between personality structure and emotional state of two different groups: heroin addicts and recreate drug abusers. METHODS: The total of 288 (219 males and 69 females; 191 heroin addicts and 97 recreate drug users clients of Centre for the prevention and treatment of drug abuse in Rijeka completed Eysenck's Personality Questionnaire (EPQ R/A, Beck's Anxiety Inventory (BAI and Beck's Depression Inventory (BDI. Their average age was 22. RESULTS: In the group of heroin addicts, higher levels of anxiety and depression were significantly correlated with higher levels of psychoticism, neuroticism, criminality and addiction. In the group of recreate drug users, higher extraversion and social conformity were determined. Furthermore, in the first group was found even higher depression. However when the anxiety level was compared between these two groups, there was no significant difference. CONCLUSION: Overall, the findings implied that the used measurement instruments could serve as the useful diagnostic tools that could ensure advantageous treatment directions.

  1. A Mathematical Images Group Model to Estimate the Sound Level in a Close-Fitting Enclosure

    Directory of Open Access Journals (Sweden)

    Michael J. Panza

    2014-01-01

    Full Text Available This paper describes a special mathematical images model to determine the sound level inside a close-fitting sound enclosure. Such an enclosure is defined as the internal air volume defined by a machine vibration noise source at one wall and a parallel reflecting wall located very close to it and acts as the outside radiating wall of the enclosure. Four smaller surfaces define a parallelepiped for the volume. The main reverberation group is between the two large parallel planes. Viewed as a discrete line-type source, the main group is extended as additional discrete line-type source image groups due to reflections from the four smaller surfaces. The images group approach provides a convergent solution for the case where hard reflective surfaces are modeled with absorption coefficients equal to zero. Numerical examples are used to calculate the sound pressure level incident on the outside wall and the effect of adding high absorption to the front wall. This is compared to the result from the general large room diffuse reverberant field enclosure formula for several hard wall absorption coefficients and distances between machine and front wall. The images group method is shown to have low sensitivity to hard wall absorption coefficient value and presents a method where zero sound absorption for hard surfaces can be used rather than an initial hard surface sound absorption estimate or measurement to predict the internal sound levels the effect of adding absorption.

  2. Health workforce remuneration: Comparing wage levels, ranking, and dispersion of 16 occupational groups in 20 countries

    NARCIS (Netherlands)

    K.G. Tijdens (Kea); D.H. de Vries (Daniel); S.M. Steinmetz

    2013-01-01

    textabstractBackground: This article represents the first attempt to explore remuneration in Human Resources for Health (HRH), comparing wage levels, ranking and dispersion of 16 HRH occupational groups in 20 countries (Argentina, Belarus, Belgium, Brazil, Chile, Colombia, the Czech Republic, Finlan

  3. Health Workforce Remuneration: comparing wage levels, ranking and dispersion of 16 occupational groups in 20 countries

    NARCIS (Netherlands)

    Tijdens, K.; de Vries, D.H.; Steinmetz, S.

    2013-01-01

    Background This article represents the first attempt to explore remuneration in Human Resources for Health (HRH), comparing wage levels, ranking and dispersion of 16 HRH occupational groups in 20 countries (Argentina, Belarus, Belgium, Brazil, Chile, Colombia, the Czech Republic, Finland, Germany, I

  4. Global dimensions for Lie groups at level k and their conformally exceptional quantum subgroups

    CERN Document Server

    Coquereaux, Robert

    2010-01-01

    We obtain formulae giving global dimensions for fusion categories defined by Lie groups G at level k and for the associated module-categories obtained via conformal embeddings. The results can be expressed in terms of Lie quantum superfactorials of type G. The later are related, for the type Ar, to the quantum Barnes function.

  5. The Effect of Group Logotherapy on Meaning in Life and Depression Levels of Iranian Students

    Science.gov (United States)

    Robatmili, Somaye; Sohrabi, Faramarz; Shahrak, Mohammad Ali; Talepasand, Siavash; Nokani, Mostafa; Hasani, Mohaddese

    2015-01-01

    This paper identifies the effectiveness of group logotherapy in reducing depression and increasing meaning in life levels of university students in Iran. A randomized controlled trial was conducted with a pre- post- and follow-up test design. The instruments used were the "Purpose in Life" (PIL) test and the "Beck Depression…

  6. Simulation Analysis for HB-Line Dissolver Mixing

    Energy Technology Data Exchange (ETDEWEB)

    Lee, S

    2006-03-22

    In support of the HB-Line Engineering agitator mixing project, flow pattern calculations have been made for a 90{sup o} apart and helical pitch agitator submerged in a flat tank containing dissolver baskets. The work is intended to determine maximum agitator speed to keep the dissolver baskets from contacting the agitator for the nominal tank liquid level. The analysis model was based on one dissolver basket located on the bottom surface of the flat tank for a conservative estimate. The modeling results will help determine acceptable agitator speeds and tank liquid levels to ensure that the dissolver basket is kept from contacting the agitator blade during HB-Line dissolver tank operations. The numerical modeling and calculations have been performed using a computational fluid dynamics approach. Three-dimensional steady-state momentum and continuity equations were used as the basic equations to estimate fluid motion driven by an agitator with four 90{sup o} pitched blades or three flat blades. Hydraulic conditions were fully turbulent (Reynolds number about 1 x 10{sup 5}). A standard two-equation turbulence model ({kappa},{var_epsilon}), was used to capture turbulent eddy motion. The commercial finite volume code, Fluent [5], was used to create a prototypic geometry file with a non-orthogonal mesh. Hybrid meshing was used to fill the computational region between the round-edged tank bottom and agitator regions. The nominal calculations and a series of sensitivity runs were made to investigate the impact of flow patterns on the lifting behavior of the dissolver basket. At high rotational speeds and low tank levels, local turbulent flow reaches the critical condition for the dissolver basket to be picked up from the tank floor and to touch the agitator blades during the tank mixing operations. This is not desirable in terms of mixing performance. The modeling results demonstrate that the flow patterns driven by the agitators considered here are not strong enough to

  7. Non-parametric group-level statistics for source-resolved ERP analysis.

    Science.gov (United States)

    Lee, Clement; Miyakoshi, Makoto; Delorme, Arnaud; Cauwenberghs, Gert; Makeig, Scott

    2015-01-01

    We have developed a new statistical framework for group-level event-related potential (ERP) analysis in EEGLAB. The framework calculates the variance of scalp channel signals accounted for by the activity of homogeneous clusters of sources found by independent component analysis (ICA). When ICA data decomposition is performed on each subject's data separately, functionally equivalent ICs can be grouped into EEGLAB clusters. Here, we report a new addition (statPvaf) to the EEGLAB plug-in std_envtopo to enable inferential statistics on main effects and interactions in event related potentials (ERPs) of independent component (IC) processes at the group level. We demonstrate the use of the updated plug-in on simulated and actual EEG data.

  8. Modelling the Relative Contribution of Fasting and Post-Prandial Plasma Glucose to HbA1c in Healthy and Type 2 Diabetic Subjects

    Science.gov (United States)

    Ollerton, Richard L.; Luzio, Steven D.; Owens, David R.

    2004-01-01

    Glycated haemoglobin (HbA1c) is regarded as the gold standard of glucose homeostasis assessment in diabetes. There has been much discussion in recent medical literature of experimental results concerning the relative contribution of fasting and post-prandial glucose levels to the value of HbA1c. A mathematical model of haemoglobin glycation is…

  9. Group personality during collective decision-making: a multi-level approach.

    Science.gov (United States)

    Planas-Sitjà, Isaac; Deneubourg, Jean-Louis; Gibon, Céline; Sempo, Grégory

    2015-03-01

    Collective decision-making processes emerge from social feedback networks within a group. Many studies on collective behaviour underestimate the role of individual personality and, as a result, personality is rarely analysed in the context of collective dynamics. Here, we show evidence of sheltering behaviour personality in a gregarious insect (Periplaneta americana), which is characterized by a collective personality at the group level. We also highlight that the individuals within groups exhibited consistent personality traits in their probability of sheltering and total time sheltered during the three trials over one week. Moreover, the group personality, which arises from the synergy between the distribution of behaviour profiles in the group and social amplifications, affected the sheltering dynamics. However, owing to its robustness, personality did not affect the group probability of reaching a consensus. Finally, to prove social interactions, we developed a new statistical method that will be helpful for future research on personality traits and group behaviour. This approach will help to identify the circumstances under which particular group compositions may improve the fitness of individuals in gregarious species.

  10. 2型糖尿病患者糖化血红蛋白和空腹血糖水平与视网膜病变相关性分析%Relationship between the Lever of Glycosylated Hemoglobin AIC (HbA1c) and Fasting Plasma Glucose (FPG) of Type 2 Diabetic Patients with Diabetic Retinopathy (DR)

    Institute of Scientific and Technical Information of China (English)

    赵建军; 朱武; 王华

    2011-01-01

    Objective To investigate the relationship of serum glycosylated hemoglobin Alc (HbAlc) and fasting plasma glucose of type 2 diabetie patients with diabetic retinopathy (DR). Methods 129 patients with T2DM were measured the levels of HbAlc and FPG and examined the eyes or vessel fluorescence angiography. According tq the results,the patients were divided int0 four groups : T2DM non-diabetic retinopathy (n = 77) ; diabetic retinopathy group (n = 52);non-proliferative diabetic retinopathy (n=39) and proliferative diabetic retinopathy (n=13).Measure the HbAlc and the level of FPG of all the patients according to the method of IE-HPLG and the oxidizing process of glucose. Analyze the statistics of each group. Results The levels of serum HbAlc in group DR and group NDR were significantly different from those of control group (P<0. 01). The levels of FPG rose obviously with the development and the degree of canceration of DR (P<0. 01) ,the relevant analysis of FPG and HbAlc in each group,group DR (group NPDR and group PDR) shows obvious relevance (r= 0. 792),group NDR also shows obvious relevance (r =0. 684). Conclusion The levels of serum HbAlc and FPG in T2DM patients are positively correlated to the occurrence ,development and the degree of retinopathy , which is the important monitoring index of diabetes mellitus,DM and the monitor of complication.%目的 探讨2型糖尿病(T2DM)患者糖化血红蛋白(HbA1c)和空腹血糖(FPG)水平与视网膜病变(DR)的相关性.方法 对129例T2DM患者进行眼底检查或眼底血管荧光素造影检查,并依据眼底检查结果分组,确定T2DM无视网膜病变(NDR)组患者77例,DR组患者52例,其中非增生性视网膜病变(NPDR)组患者39例,增生性视网膜病变(PDR)组患者13例.所有研究对象HbA1c和FPG水平采用IE-HPLC法及葡萄糖氧化酶法进行定量测定,各组间进行统计学分析.结果 DR组HbA1c水平与NDR组和正常对照组比较差异有统计学显著性意义(P<0

  11. Comparison of serum levels of copper and zinc among multiple sclerosis patients and control group.

    Directory of Open Access Journals (Sweden)

    Behnaz Sedighi

    2013-12-01

    Full Text Available There have been several studies done on the role of metals in the occurrence of multiple sclerosis (MS disease, but their roles have not been confirmed yet. Because of the lack of information on this issue, this study compared the serum level of copper and zinc in MS patients with their levels in a control group.This was an analytical, cross-sectional study conducted in Kerman (a medium size city, Iran. We assessed the serum level of copper and zinc in 58 MS patients and 39 healthy individuals, who were selected from the relatives of cases and matched for age and sex.The average serum level of Copper in cases and controls were 93.7 and 88.9 ml/dl, respectively. The corresponding numbers for Zinc were 36.7 and 40.9 ml/dl, respectively. There was no significant difference between the two groups (copper: P = 0.459; zinc: P = 0.249.The groups were matched for age, sex, and family. However, we did not find a considerable difference between the level of these metals in MS patients and controls.

  12. Is There a Relationship Between 25-Hydroksyvitamin D3 Levels and Diabetes Control?

    Directory of Open Access Journals (Sweden)

    Maide Hacer Tekin

    2016-03-01

    Full Text Available Aim: The aim of this study was to evaluate the relationship between hemoglobin A1c (HbA1c and 25-hydroxyvitamin D levels and to investigate the difference in this relationship between genders. Methods: In this study, we retrospectively evaluated fasting blood glucose, HbA1c, 25-hydroxyvitamin D and parathyroid hormone levels in 3675 subjects (660 male, 3015 female. The subjects were divided into two groups according to sex and into three groups according to HbA1c levels as pre-diabetics (5.7-6.4%, diabetics (6.5-8.0% and uncontrolled diabetics (>8.1%. Individuals with an HbA1c value between 4.0% and 5.6% who had no history of diabetes mellitus constituted the control group. Results: Significantly low levels of 25-hydroxyvitamin D were detected in female uncontrolled diabetics (p<0.001. However, no difference found in males between the groups A downward trend in 25-hydroxyvitamin D levels was observed to start in prediabetes period. There was no significant difference in parathyroid hormone levels between the groups. Conclusion: In addition to HbA1c levels, 25-hydroxyvitamin D levels should be investigated and considered especially in follow-up of diabetic female patients.

  13. Posterior transverse interarch discrepancy on HbE β thalassemia patients

    Directory of Open Access Journals (Sweden)

    Yuniar Zen

    2011-03-01

    Full Text Available Background: One of the symptoms that often arises on thalassemia patients is disharmony dentofacial, class II skeletal malocclusion, as a result of the malrelation of maxilla and mandible. This malrelation can be affected by either maxillary bone position, dentoalveolar maxillary position, mandibular bone position, dentoalveolar mandibular position, or combinations of those components. Purpose: The study was aimed to examine whether there is posterior transverse interarch discrepancy on the HbE β thalassemia patients or not. Methods: This study is an observational research with cross-sectional design. The sample consisted of 33 HbE β thalassemia patients and 33 non-thalassemia patients as a control group aged 12–14 years. Lateral cephalogram was carried out and dental casts of maxillary and mandibular dental arches were also taken in all of those patients. Results: There was no difference between the maxillary intermolar width of the HbE β thalassemia patients and that of the normal ones, but the mandibular intermolar width of the HbE β thalassemia patients was significantly smaller than that of the normal ones. Beside that, posterior transverse interarch discrepancy of of the HbE β thalassemia patients was significantly greater than that of the normal ones, which showed great difference between maxillary and mandibular intermolar widths. Conclusion: Posterior transverse interarch discrepancy of the HbE β thalassemia patients was different from that of the normal ones. The dentofacial abnormalities on the HbE β thalassemia patients aged 12–14 years primarily was due to disporposional dentofacial growth in the vertical, sagittal, and transversal directions, especially in the posterior region.Latar belakang: Salah satu akibat yang sering timbul pada penderita talasemia adalah disharmoni dentofasial berupa maloklusi skeletal kelas II yang merupakan kelainan hubungan maksila dan mandibula. Malrelasi ini dapat dipengaruhi oleh posisi

  14. The group-level consequences of sexual conflict in multigroup populations.

    Directory of Open Access Journals (Sweden)

    Omar Tonsi Eldakar

    Full Text Available In typical sexual conflict scenarios, males best equipped to exploit females are favored locally over more prudent males, despite reducing female fitness. However, local advantage is not the only relevant form of selection. In multigroup populations, groups with less sexual conflict will contribute more offspring to the next generation than higher conflict groups, countering the local advantage of harmful males. Here, we varied male aggression within- and between-groups in a laboratory population of water striders and measured resulting differences in local population growth over a period of three weeks. The overall pool fitness (i.e., adults produced of less aggressive pools exceeded that of high aggression pools by a factor of three, with the high aggression pools essentially experiencing no population growth over the course of the study. When comparing the fitness of individuals across groups, aggression appeared to be under stabilizing selection in the multigroup population. The use of contextual analysis revealed that overall stabilizing selection was a product of selection favoring aggression within groups, but selected against it at the group-level. Therefore, this report provides further evidence to show that what evolves in the total population is not merely an extension of within-group dynamics.

  15. Dietary determinants for Hb-acrylamide and Hb-glycidamide adducts in Danish non-smoking women

    DEFF Research Database (Denmark)

    Outzen, Malene; Egeberg, Rikke; Dragsted, Lars

    2011-01-01

    percentile 8, 95th percentile 49). Of the dietary factors studied, intakes of coffee and chips were statistically significantly associated with a 4 % per 200 g/d (95 % CI 2, 7; P ... in Hb-AA. Intakes of coffee and biscuits/crackers were statistically significantly associated with a 3 % per 200 g/d (95 % CI 1, 6; P = 0·005) and 12 % per 10 g/d (95 % CI 3, 23; P = 0·01) higher Hb-GA, respectively. This model explained 12 % of the variation in Hb-GA. In conclusion, only a few dietary...

  16. Hb D/Talassemia beta associada à anemia crônica Hb D/ Beta thalassaemia associated with chronic anaemia

    Directory of Open Access Journals (Sweden)

    Paulo C. Naoum

    2002-03-01

    Full Text Available We describe a case of Hb D/Beta thalassemia associated with chronic anemia. Hematological analyses performed in a patient with chronic anemia demonstrating microcytosis and hypochromic in his erythrocytes. Specific laboratory diagnosis performed by alkaline and acid electrophoresis, and fetal determination by alkali resistance, indicated it to be Hb D associated with beta thalassemia. Analyses carried out on his family (father, mother and brother confirmed the suspected diagnosis. Hb D/Beta thalassemia is a very rare interaction in the Brazilian population, and its determination required specific laboratorial techniques and hematological analyses.

  17. A network-level explanation for the differences in HIV prevalence in South Africa's racial groups.

    Science.gov (United States)

    Kenyon, Chris; Dlamini, Sipho; Boulle, Andrew; White, Richard G; Badri, Motasim

    2009-09-01

    Analyses of individual-level risk factors have not been able to adequately explain why HIV has spread so extensively in southern Africa and why this has occurred especially within certain racial or ethnic groups. Using data from a longitudinal study of a representative sample of adolescents aged 14-22 living in Cape Town, South Africa, this article presents evidence of how differences in individual-level risk factors as well as sexual network structures between different racial or ethnic groups may help explain the differential spread of HIV in South Africa. Particular emphasis is placed on how levels of partner concurrency, respondent concurrency, mutual concurrency, serial concurrency and numbers of sexual partners and an average early age of sexual debut combine in different ways in the different racial or ethnic groups to create networks of sexual partnerships that differ in the density of their interconnections and hence potential for HIV spread. These network-level differences offer a potential explanation for the observed generalised HIV epidemic seen among the population of black South Africans.

  18. Rubber particle proteins, HbREF and HbSRPP, show different interactions with model membranes.

    Science.gov (United States)

    Berthelot, Karine; Lecomte, Sophie; Estevez, Yannick; Zhendre, Vanessa; Henry, Sarah; Thévenot, Julie; Dufourc, Erick J; Alves, Isabel D; Peruch, Frédéric

    2014-01-01

    The biomembrane surrounding rubber particles from the hevea latex is well known for its content of numerous allergen proteins. HbREF (Hevb1) and HbSRPP (Hevb3) are major components, linked on rubber particles, and they have been shown to be involved in rubber synthesis or quality (mass regulation), but their exact function is still to be determined. In this study we highlighted the different modes of interactions of both recombinant proteins with various membrane models (lipid monolayers, liposomes or supported bilayers, and multilamellar vesicles) to mimic the latex particle membrane. We combined various biophysical methods (polarization-modulation-infrared reflection-adsorption spectroscopy (PM-IRRAS)/ellipsometry, attenuated-total reflectance Fourier-transform infrared (ATR-FTIR), solid-state nuclear magnetic resonance (NMR), plasmon waveguide resonance (PWR), fluorescence spectroscopy) to elucidate their interactions. Small rubber particle protein (SRPP) shows less affinity than rubber elongation factor (REF) for the membranes but displays a kind of "covering" effect on the lipid headgroups without disturbing the membrane integrity. Its structure is conserved in the presence of lipids. Contrarily, REF demonstrates higher membrane affinity with changes in its aggregation properties, the amyloid nature of REF, which we previously reported, is not favored in the presence of lipids. REF binds and inserts into membranes. The membrane integrity is highly perturbed, and we suspect that REF is even able to remove lipids from the membrane leading to the formation of mixed micelles. These two homologous proteins show affinity to all membrane models tested but neatly differ in their interacting features. This could imply differential roles on the surface of rubber particles.

  19. JDL level 5 fusion model: user refinement issues and applications in group tracking

    Science.gov (United States)

    Blasch, Erik P.; Plano, Susan

    2002-07-01

    The 1999 Joint Director of Labs (JDL) revised model incorporates five levels for fusion methodologies including level 0 for preprocessing, level 1 for object refinement, level 2 for situation refinement, level 3 for threat refinement, and level 4 for process refinement. The model was developed to define the fusion process. However, the model is only for automatic processing of a machine and does not account for human processing. Typically, a fusion architecture supports a user and thus, we propose a Level 5 User refinement to delineate the human from the machine in the process refinement. Typical human in the loop models do not deal with a machine fusion process, but only present the information to the human on a display. We seek to address issues for designing a fusion system which supports a user: trust, workload, attention and situation awareness. In this paper, we overview the need for a Level 5, the issues concerning the human for realizable fusion architectures, and examples where the human is instrumental in the fusion process such as group tracking.

  20. [Effects of nitrogen and phosphorus fertilizer on atrazine degradation and detoxification by degrading strain HB-5].

    Science.gov (United States)

    Su, Jun; Zhu, Lu-Sheng; Li, Xu-Hua; Wang, Jun; Xie, Hui; Wang, Jin-Hua; Wang, Qi; Jia, Wen-Tao

    2010-10-01

    An atrazine-degrading strain HB-5 was used as a bacteria for biodegradation. Treatments of soil with nitrogen single, phosphate single and nitrogen phosphate together with HB-5 were carried out for degradation and eco-toxicity test; then, relationship between atrazine degradation rate and soil available nitrogen, available phosphorus were discussed. Atrazine residues were determined by HPLC; available nitrogen was determined with alkaline hydrolysis diffusion method; available phosphorus was determined with 0.5 mol/L-NaHCO3 extraction and molybdenum stibium anti-color method, and toxicity test was carried out with micronucleus test of Vicia faba root tip cells. The results showed that: After separately or together application, nitrogenous and phosphorous fertilizers could significantly accelerate atrazine degradation than soil with HB-5 only. On day 5, the order of atrazine degradation was ANP > AP > AN > A; 7 days later, no statistically significant differences were found between treatments. The available nitrogen and phosphorus level in soil reduced as the degradation rate increased in the soil. The soil of eco-toxicity test results indicated that the eco-toxicity significantly reduced with the degradation of atrazine by HB-5, and the eco-toxicity on treatments of soil with fertilizer were all below the treatments without fertilizer. On day 5, the order of eco-toxicity was ANP atrazine degradation in soil but also could reduce the soil eco-toxicity effects that atrazine caused. All these results could be keystone of atrazine pollution remediation in contaminated soil in the future.

  1. HbA2 measurements in β-thalassemia and in other conditions

    Directory of Open Access Journals (Sweden)

    Giovanni Ivaldi

    2014-09-01

    Full Text Available Quite a few papers have been written on the significance of elevated hemoglobin (Hb A2 as a parameter for the diagnosis of β-thalassemia trait, on the cutoff values to be used in diagnostics and on the significance and effects of factors reducing or elevating the expression of HbA2 and last but not least on the need for reliable measurement methods and precise calibrations with accurate standards. However, little has been published on the causes that elevate or reduce the HbA2 levels in β- and a-thalassemia and in other conditions. For a better understanding of the value of a precise measurement of this parameter we summarize and elucidate in this review the direct and indirect mechanisms that cause the variations in HbA2 expression and that influence the value of this parameter in particular conditions. We conclude by explaining the advantages and disadvantages of trusting on a precise measurement in the complete diagnostic contest.

  2. Aggression and cortisol levels in three different group housing routines for lactating sows.

    Science.gov (United States)

    Thomsson, Ola; Bergqvist, Ann-Sofi; Sjunnesson, Ylva; Eliasson-Selling, Lena; Lundeheim, Nils; Magnusson, Ulf

    2015-02-18

    Lactating sows in Swedish organic piglet production are commonly group-housed with piglets in a multi-suckling pen within 14 days after farrowing. Nursing behaviour may be disturbed when lactating sows are moved to a new environment and mixed with other sows, as they spend more time fighting with other sows and exploring the new surroundings. This can disrupt the inhibitory effect of suckling on ovarian activity and increase the risk of lactational oestrus, making efficient reproductive management difficult. Therefore this study evaluated aggression and levels of the stress hormone cortisol in lactating sows group-housed together with their piglets at one (W1), two (W2) or three (W3) weeks post farrowing. There was no significant difference (P > 0.05) between the three management routines (W1, W2, W3) regarding number of attacks initiated or received in the mixed group. After mixing, W2 sows had a lower number of shoulder scratches (P sows. Among the W3 sows, there was a lower (P sows were group housed compared to when they were individually housed. The cortisol response, measured as variation in cortisol concentration in saliva, was also lower (P sows compared with W1 sows. For all management routines, sows already living in the new environment (resident sows) initiated more attacks (P sows entering the new environment (intruder sows). Overall, multiparous sows initiated more attacks and received fewer attacks than primiparous sows (P sows at three weeks post farrowing is less stressful than mixing and group housing sows at one week post farrowing. The results also indicate that parity and whether a sow is a resident or intruder in the group housing environment may have an effect on aggression levels when sows are group-housed.

  3. 可降解材料P(3HB-co-4HB)的性能分析%Property Analysis of Biodegradable Material P(3HB-co-4HB)

    Institute of Scientific and Technical Information of China (English)

    张竞; 褚庭亮

    2012-01-01

    本文对聚(3-羟基丁酸酯-co-4-羟基丁酸酯)(P(3HB-co-4HB))的基本性能和降解性能进行了研究,分析了降解机理及降解影响因素.通过差示扫描量热仪、偏光显微镜、拉力机等表征了P(3HB-co-4HB)的结晶及力学性能.采用扫描电子显微镜,并通过热失重分析,考察了降解过程中表面形态和样品质量等的变化.结果表明:P(3HB-co-4HB)是一种环境友好、可降解的环保材料,其物理性能、球晶尺寸和球晶形态随着4HB含量的不同而改变.

  4. Effects of a healthier snack on snacking habits and glycated Hb (HbA1c): a 6-week intervention study.

    Science.gov (United States)

    Yan, Mary R; Parsons, Andrew; Whalley, Gillian A; Rush, Elaine C

    2016-12-01

    Dietary behaviour modification may change eating habits and reduce the impact of poor nutrition. This study aimed to evaluate the effects of daily consumption of a healthier snack bar on snacking habits and glycated Hb (HbA1c) within a 6-week intervention. In all, twenty-eight participants were randomly allocated to two groups to either consume the bars as the main snack for 6 weeks (n 14) or receipt of the bars was delayed for 6 weeks (n 14) following a stepped-wedge design. All participants had HbA1c concentrations measured at weeks -1, 0, 4, 6, 10 and 12. A short dietary habits questionnaire was self-completed at weeks 0, 6 and 12. Participants consumed the bars they received instead of other snacks, and found that the healthier snack bar was acceptable as part of their daily dietary pattern. Over the 12 weeks, there was a significant reduction in intake of biscuits, cakes and pies (approximately 2 servings/week, Psnack intervention and a trend towards a favourable effect on glucose homoeostasis. Habitual snacking behaviour has the potential to be improved through changes in the food supply, and in the longer term may reduce the impact of poor nutrition on public health.

  5. Screening for recombinant human erythropoietin using [Hb], reticulocytes, the OFF(hr score), OFF(z score) and Hb(z score): status of the Blood Passport.

    Science.gov (United States)

    Bornø, Andreas; Aachmann-Andersen, Niels J; Munch-Andersen, Thor; Hulston, Carl J; Lundby, Carsten

    2010-06-01

    Haemoglobin concentration ([Hb]), reticulocyte percentage (retic%) and OFF(hr score) are well-implemented screening tools to determine potential recombinant human erythropoietin (rHuEpo) abuse in athletes. Recently, the International Cycling Union implemented the OFF(z score) and the Hb(z score) in their anti-doping testing programme. The aim of this study is to evaluate the sensitivity of these indirect screening methods. Twenty-four human subjects divided into three groups with eight subjects each (G1; G2 and G3) were injected with rHuEpo. G1 and G2 received rHuEpo for a 4-week period with 2 weeks of "boosting" followed by 2 weeks of "maintenance" and a wash-out period of 3 weeks. G3 received rHuEpo for a 10-week period (boost = 3 weeks; maintenance = 7 weeks; wash out = 1 week). Three, seven and eight of the 24 volunteers exceeded the cut-off limits for OFF(hr score), [Hb] and retic%, respectively. One subject from G1, nobody from G2, and seven subjects from G3 exceeded the cut-off limit for Hb(z score.) In total, ten subjects exceeded the cut-off limit for the OFF(z score); two subjects from G1, two subjects from G2 and six subjects from G3. In total, indirect screening methods were able to indicate rHuEpo injections in 58% of subjects. However, 42% of our rHuEpo-injected subjects were not detected. It should be emphasised that the test frequency in real world anti-doping is far less than the present study, and hence the detection rate will be lower.

  6. Effect of individual and group housing of mice on the level of radioresistance

    Directory of Open Access Journals (Sweden)

    Dorozhkina O.V.

    2015-12-01

    Full Text Available Aim: to examine the effect of individual and group housing of mice on radioresistance. Material and methods. Effects of individual and group housing of mice on immunity and blood systems were studied on ICR (CD-1 and C57BI6 male mice before and after proton irradiation. Results. Group housing of intact animals resulted in a decline in the number of nucleated cells in the femur bone marrow and thymus mass. The irradiation with proton with energy of 171 MeV at a dose of 1 Gy causes a statistically significant greater reduction of the number of nucleated cells in the femur bone marrow in group-housed mice. A trend toward greater safety of the number of leukocytes in the peripheral blood and higher proliferative activity of bone marrow cells, as well as lower level of aberrant mitoses have been noted in individually-housed mice. Reduction processes in the recovery period of radiation sickness take place at a greater rate in group-housed mice. Conclusion. Group housing of male mice causes increased sensitivity of the blood and immunity systems to the effects of radiation and at the same time accelerates processes of radiation recovery.

  7. Trimester-specific reference intervals for haemoglobin A(1c) (HbA(1c)) in pregnancy.

    LENUS (Irish Health Repository)

    O'Connor, Catherine

    2011-11-26

    Abstract Background: Diabetes in pregnancy imposes additional risks to both mother and infant. These increased risks are considered to be primarily related to glycaemic control which is monitored by means of glycated haemoglobin (HbA(1c)). The correlation of HbA(1c) with clinical outcomes emphasises the need to measure HbA(1c) accurately, precisely and for correct interpretation, comparison to appropriately defined reference intervals. Since July 2010, the HbA(1c) assay in Irish laboratories is fully metrologically traceable to the IFCC standard. The objective was to establish trimester-specific reference intervals in pregnancy for IFCC standardised HbA(1c) in non-diabetic Caucasian women. Methods: The authors recruited 311 non-diabetic Caucasian pregnant (n=246) and non-pregnant women (n=65). A selective screening based on risk factors for gestational diabetes was employed. All subjects had a random plasma glucose <7.7 mmol\\/L and normal haemoglobin level. Pregnancy trimester was defined as trimester 1 (T1, n=40) up to 12 weeks +6 days, trimester 2 (T2, n=106) 13-27 weeks +6 days, trimester 3 (T3, n=100) >28 weeks to term. Results: The normal HbA(1c) reference interval for Caucasian non-pregnant women was 29-37 mmol\\/mol (Diabetes Control and Complications Trial; DCCT: 4.8%-5.5%), T1: 24-36 mmol\\/mol (DCCT: 4.3%-5.4%), T2: 25-35 mmol\\/mol (DCCT: 4.4%-5.4%) and T3: 28-39 mmol\\/mol (DCCT: 4.7%-5.7%). HbA(1c) was significantly decreased in trimesters 1 and 2 compared to non-pregnant women. Conclusions: HbA(1c) trimester-specific reference intervals are required to better inform the management of pregnancies complicated by diabetes.

  8. Capillarys 2 Flex Piercing: Analytical performance assessment according to CLSI protocols for HbA1c quantification.

    Science.gov (United States)

    Doggui, Radhouene; Abdelhafidh Sahli, Chaïma; Aissa, Wassef Lotfi; Hammami, Maroua; Ben Sedrine, Maha; Mahjoub, Rahma; Zouaoui, Khemais; Daboubi, Rim; Siala, Hajer; Messaoud, Taieb; Bibi, Amina

    2017-09-01

    HbA1c is used for monitoring diabetic balance. In this paper we report an assessment of the analytical performances of Capillarys 2 Flex Piercing (C2FP) for HbA1c measurement using CE (Capillary Electrophoresis). CLSI (Clinical and Laboratory Standard Institute) protocols are used for the evaluation of apparatus performances: precision, linearity, method comparison, trueness and common interferences. HbA1c CVs average in intra-assay was 1.6% between run imprecision CV ranged from 0.1 to 1.8%. The linearity was demonstrated between 4.7 and 15.0%. The comparison study revealed that Bland Altman plot mean difference was equal to -0.03 (CI 95% (-0.05 to -0.0003)) and Passing-Bablok regression intercept was -0.05, CI95%(-0.13 -  -0.05); slope: 1.00, CI95%[1.00-1.01]. A strong correlation (r > 0.99) was proved. No significant effects of hemoglobin variants were seen with CE on HbA1c measurement. No problem related to sample-to-sample carry over was noted. No interferences of LA1c and cHb were observed. CE allowed quantification of HbA1c even at low level of total hemoglobin (40 g/L) in contrast to HPLC. Furthermore, this analyzer offered the opportunity of quantifying the HbA2 simultaneously with HbA1c . This evaluation showed that C2FP is a convenient system for the control of diabetes and the detection of hemoglobinopathies. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  9. Level of choreographic preparedness of sportsmen of different age groups in sports aerobics

    Directory of Open Access Journals (Sweden)

    Valentina Todorova

    2016-08-01

    Full Text Available Purpose: the analysis of choreographic preparedness of different age groups of sportsmen on sports aerobics. Material & Methods: videos of competitive programs of the sportsmen, who are specialized in aerobics, different age groups, method of expert evaluations are used for the quantitative analysis of choreographic preparedness; methods of mathematical analysis and synthesis are used for the determination of level of choreographic preparedness of sportsmen. Results: the level of choreographic preparedness is determined on the basis of the rating scale of the criteria of implementation of the competitive programs (Competition rules of 2013–2016 of teams-participants of the Championship of Ukraine for sports aerobics. Conclusions: indicators, to which it is necessary to pay attention in the course of choreographic preparation at stages of long-term training of sportsmen, are defined.

  10. Increased level of glycated hemoglobin portends high risk of coronary artery stenosis

    Institute of Scientific and Technical Information of China (English)

    ZENG FAN-fang; WANG Li-li; LONG Juan; YI Wen-ya; LUO Ying

    2016-01-01

    Background Increased level of glycated hemoglobin (HbA1c) is associated with higher incidence of coronary artery disease (CAD) in the diabetics.However,the relationship between HbAlc and the risk of coronary artery stenosis in the non-diabetics is controversial.Methods A retrospective research was conducted on 338 enrolled participants who have undergone 2 times of coronary angiographic examination within the past year.Clinical and laboratory variables at the initial and the second time of admission were collected.According to the initial median HbA1c level,all participants were divided into two groups named lower and higher groups.The relationship between HbA1c level and the risk of coronary artery stenosis over time was evaluated.Results The initial values of HbA1c in lower and upper groups were 5.78 ± 0.35% and 6.21 ± 0.32% (P < 0.05).As compared to the lower group,the percentages of male and smoking participants,and the serum level of CRP were significantly higher in the higher group (P < 0.05).Other traditional risk factors were comparable between the two groups.There were 54.2% and 55.2% participants with single vessel stenosis,and 45.8% and 44.8% with multiple vessel stenoses,respectively in the two groups without significant difference.The second time of admission,were 308.5± 25.4 days (lower group) and 300.7 ± 30.1 days (higher group) from the initial admission.Although no significant changes of HbA1c level were observed when compared to initial,HbA1c level in the higher group was still significantly higher in comparison to the lower group (6.24 ± 0.39% vs.5.80 ± 0.36%,P =0.008).The percentage of new coronary artery stenosis (≥ 50% stenosis) was higher in the higher group than that in the lower group (41.7% vs.32.3%,P < 0.001).Multivariate regression analyses suggested that HbAlc remained independent factor associated with coronary artery stenoses after extensive adjustment for risk factors.Conclusion In the nondiabetics

  11. Baseline HbA1c to Identify High-Risk Gestational Diabetes: Utility in Early vs Standard Gestational Diabetes.

    Science.gov (United States)

    Sweeting, Arianne N; Ross, Glynis P; Hyett, Jon; Molyneaux, Lynda; Tan, Kris; Constantino, Maria; Harding, Anna Jane; Wong, Jencia

    2017-01-01

    The increasing prevalence of gestational diabetes mellitus (GDM) necessitates risk stratification directing limited antenatal resources to those at greatest risk. Recent evidence demonstrates that an early pregnancy glycated hemoglobin (HbA1c ≥5.9% (41 mmol/mol) predicts adverse pregnancy outcomes. To determine the optimal HbA1c threshold for adverse pregnancy outcomes in GDM in a treated multiethnic cohort and whether this differs in women diagnosed 5.9% (41 mmol/mol) was associated with the greatest risk of large-for-gestational-age (odds ratio [95% confidence interval] = 2.7 [1.5-4.9]), macrosomia (3.5 [1.4-8.6]), cesarean section (3.6 [2.1-6.2]), and hypertensive disorders (2.6 [1.1-5.8]). In early GDM, similar HbA1c associations were seen; however, lower HbA1c correlated with the greatest risk of small-for-gestational-age (P trend = 0.004) and prevalence of neonatal hypoglycemia. Baseline HbA1c >5.9% (41 mmol/mol) identifies an increased risk of large-for-gestational-age, macrosomia, cesarean section, and hypertensive disorders in standard GDM. Although similar associations are seen in early GDM, higher HbA1c levels do not adequately capture risk-limiting utility as a triage tool in this cohort.

  12. Clinical Significance of HbAlc Detection in the Diagnostic of Diabetic Mellitus%糖化血红蛋白测定在糖尿病诊断中的临床意义

    Institute of Scientific and Technical Information of China (English)

    赵洪波

    2016-01-01

    Objective To investigate the value of HbAlc detection in the diagnostic of diabetic melitus.Methods74 cases of patients with diabetes melitus (observation group) and 74 healthy subjects (control group), to compare the result of two groups.Results The fasting blood glucose of observation group which was(9.55±1.50)mmol/L and HbAlc levels, (8.45±1.75)%, were higher than control group [(5.06±0.64)mmol/L,(4.30±1.04)%] (P<0.05), there was a positive correlation between the blood glucose and HbAlc levels.Conclusion HbAlc can diagnose diabetes melitus effectively.%目的:观察糖化血红蛋白诊断糖尿病的价值。方法选择74例糖尿病患者(观察组),并选择同期74例健康体检者(对照组),比较两组检测结果。结果观察组空腹血糖(9.55±1.50)mmol/L、糖化血红蛋白(8.45%±1.75%)等水平均高于对照组[(5.06±0.64)mmol/L,(4.30%±1.04%)](P<0.05);观察组血糖与糖化血红蛋白水平存在正相关。结论糖化血红蛋白可有效诊断糖尿病。

  13. HB-Line Plutonium Oxide Data Collection Strategy

    Energy Technology Data Exchange (ETDEWEB)

    Watkins, R. [Savannah River Nuclear Solutions; Varble, J. [Savannah River Nuclear Solutions; Jordan, J. [Savannah River Nuclear Solutions

    2015-05-26

    HB-Line and H-Canyon will handle and process plutonium material to produce plutonium oxide for feed to the Mixed Oxide Fuel Fabrication Facility (MFFF). However, the plutonium oxide product will not be transferred to the MFFF directly from HB-Line until it is packaged into a qualified DOE-STD-3013-2012 container. In the interim, HB-Line will load plutonium oxide into an inner, filtered can. The inner can will be placed in a filtered bag, which will be loaded into a filtered outer can. The outer can will be loaded into a certified 9975 with getter assembly in compliance with onsite transportation requirement, for subsequent storage and transfer to the K-Area Complex (KAC). After DOE-STD-3013-2012 container packaging capabilities are established, the product will be returned to HB-Line to be packaged into a qualified DOE-STD-3013-2012 container. To support the transfer of plutonium oxide to KAC and then eventually to MFFF, various material and packaging data will have to be collected and retained. In addition, data from initial HB-Line processing operations will be needed to support future DOE-STD-3013-2012 qualification as amended by the HB-Line DOE Standard equivalency. As production increases, the volume of data to collect will increase. The HB-Line data collected will be in the form of paper copies and electronic media. Paper copy data will, at a minimum, consist of facility procedures, nonconformance reports (NCRs), and DCS print outs. Electronic data will be in the form of Adobe portable document formats (PDFs). Collecting all the required data for each plutonium oxide can will be no small effort for HB-Line, and will become more challenging once the maximum annual oxide production throughput is achieved due to the sheer volume of data to be collected. The majority of the data collected will be in the form of facility procedures, DCS print outs, and laboratory results. To facilitate complete collection of this data, a traveler form will be developed which

  14. 门多萨假单胞菌DS04-T对Poly(3HB-co-4HB)的降解研究%Degradation of Poly(3HB-co-4HB) by pseudomonas mendocina DS04-T

    Institute of Scientific and Technical Information of China (English)

    李琳琳; 高佳; 杨翔华; 王战勇

    2012-01-01

    考察了门多萨假单胞菌DS04-T对Poly(3HB-co-4 HB)的降解行为.以Poly(3HB-co4HB)为唯一碳源,分别考察培养时间、培养温度、摇床转速、装液量、培养基起始pH值、接种量等因素对降解行为的影响.结合正交试验优化获得了菌株的最佳产酶条件:培养时间为28 h,培养温度为30℃,培养基初始pH值为7.3,摇床转速为150 r/min,培养基装液量为120mL(250mL三角瓶),接种量为1.5%(体积分数),此条件下菌株对Poly(3HB-co-4HB)的降解酶活力可达(26.2±0.7)U·mL-1.%The degradation of Poly(3HB-co-4HB) by pseudomonas mendocina DS04-T was studied. Poly(3HB-co-4HB) was used as the sole carbon source of culture medium. The influence of cultivation time, cultivation temperature, shaking revolution, liquid medium volume, initial pH and inoculation content were studied. Combining single factor test and orthogonal test, the optimal degrading enzyme production conditions were chosen as follows: cultivation time is 28 h, cultivation temperature is 30 C, shaking revolution is 150 r/min, initial pH is 7. 3, culture medium volume is 120 mL and inoculation content is 1. 5% (V/V). Under the optimized condition, the degradation enzyme activity reaches (26. 2 + 0. 7) U · mL-1.

  15. Variables involved in the discordance between HbA1c and fructosamine: the glycation gap revisited.

    Directory of Open Access Journals (Sweden)

    Carles Zafon

    Full Text Available AIMS: Glycation gap (GG is defined as the difference between the measured level of HbA1c and the level that would be predicted from its regression on the fructosamine level. The aims of the study were: 1 To determine the reproducibility and consistency of GC; 2 To discover factors related to GG value. Given that metformin might increase glucose transport through the erythrocyte membrane, this treatment was also considered in the analyses of the results. METHODS: GG was calculated in two blood samples separated 30.6 (SD 7.3 weeks, obtained in 508 type 2 diabetic patients. The following variables were considered: HbA1c, fructosamine, glucose, creatinine, hematological parameters and treatment with metformin. Multivariate and logistic regression analyses were performed to explore the variables independently related to CG. RESULTS: GG was reproducible and consistent over time. Creatinine, mean corpuscular hemoglobin concentration (MCHC, and glycemia (inverse relationship; and HbA1c and treatment with metformin (direct relationship were independently related to GG. Patients treated with metformin showed higher HbA1c values, despite similar fructosamine concentrations, than patients not treated with the drug. CONCLUSIONS: GG is independently related to serum levels of creatinine, MCHC and treatment with metformin. The spurious effect of metformin on Hb glycation could have serious clinical implications and should be considered when interpreting the results of clinical trials.

  16. Variables involved in the discordance between HbA1c and fructosamine: the glycation gap revisited.

    Science.gov (United States)

    Zafon, Carles; Ciudin, Andreea; Valladares, Silvia; Mesa, Jordi; Simó, Rafael

    2013-01-01

    Glycation gap (GG) is defined as the difference between the measured level of HbA1c and the level that would be predicted from its regression on the fructosamine level. The aims of the study were: 1) To determine the reproducibility and consistency of GC; 2) To discover factors related to GG value. Given that metformin might increase glucose transport through the erythrocyte membrane, this treatment was also considered in the analyses of the results. GG was calculated in two blood samples separated 30.6 (SD 7.3) weeks, obtained in 508 type 2 diabetic patients. The following variables were considered: HbA1c, fructosamine, glucose, creatinine, hematological parameters and treatment with metformin. Multivariate and logistic regression analyses were performed to explore the variables independently related to CG. GG was reproducible and consistent over time. Creatinine, mean corpuscular hemoglobin concentration (MCHC), and glycemia (inverse relationship); and HbA1c and treatment with metformin (direct relationship) were independently related to GG. Patients treated with metformin showed higher HbA1c values, despite similar fructosamine concentrations, than patients not treated with the drug. GG is independently related to serum levels of creatinine, MCHC and treatment with metformin. The spurious effect of metformin on Hb glycation could have serious clinical implications and should be considered when interpreting the results of clinical trials.

  17. Study of serum syndecan-1 levels in a group of Egyptian juvenile systemic lupus erythematosus patients.

    Science.gov (United States)

    Mosaad, Naglaa Abd Elrahman; Lotfy, Hala Mohamed; Farag, Yomna Mohamed; Mahfouz, Rasha Hossam El-Din; Shahin, Rasha Mohamad Hosny

    2017-01-01

    The aim of the study was to assess the serum levels of Syndecan-1 in a group of Egyptian juvenile systemic lupus erythematosus (JSLE) patients and to study any possible associations with disease activity, renal activity and organ damage. Serum level of Syndecan-1 was assessed in 60 Egyptian JSLE patients and 30 apparently healthy age and gender matched children using ELISA. SLE Disease Activity Index-2000 (SLEDAI-2K), renal SLEDAI-2K, renal activity score and the Systemic Lupus International Collaborating Clinics/American College of Rheumatology (SLICC/ACR) Damage Index were assessed for all patients. Serum SDC-1 levels were higher in patients with JSLE than in healthy controls (p<0.001) and were positively correlated with SLEDAI-2K (p<0.001), with renal SLEDAI score (p=0.008) and renal activity score (p=0.04). So, Syndecan-1 might be used as a marker for disease activity and renal activity in JSLE patients.

  18. Distraction sneakers decrease the expected level of aggression within groups: a game-theoretic model.

    Science.gov (United States)

    Dubois, Frédérique; Giraldeau, Luc-Alain; Hamilton, Ian M; Grant, James W A; Lefebvre, Louis

    2004-08-01

    Hawk-dove games have been extensively used to predict the conditions under which group-living animals should defend their resources against potential usurpers. Typically, game-theoretic models on aggression consider that resource defense may entail energetic and injury costs. However, intruders may also take advantage of owners who are busy fighting to sneak access to unguarded resources, imposing thereby an additional cost on the use of the escalated hawk strategy. In this article we modify the two-strategy hawk-dove game into a three-strategy hawk-dove-sneaker game that incorporates a distraction-sneaking tactic, allowing us to explore its consequences on the expected level of aggression within groups. Our model predicts a lower proportion of hawks and hence lower frequencies of aggressive interactions within groups than do previous two-strategy hawk-dove games. The extent to which distraction sneakers decrease the frequency of aggression within groups, however, depends on whether they search only for opportunities to join resources uncovered by other group members or for both unchallenged resources and opportunities to usurp.

  19. Correlation between the Lactate Dehydrogenase Levels with Laboratory Variables in the Clinical Severity of Sickle Cell Anemia in Congolese Patients.

    Directory of Open Access Journals (Sweden)

    Tite Minga Mikobi

    Full Text Available Sickle cell anemia is an inflammatory disease and is characterized by chronic hemolysis. We sought to evaluate the association of lactate dehydrogenase levels with specific clinical phenotypes and laboratory variables in patients with sickle cell anemia.The present cross-sectional study was conducted in Sickle Cell Centre of Yolo in Kinshasa, the Democratic Republic of Congo. Two hundred and eleven patients with Sickle Cell Anemia in steady state were recruited. Seventy-four participants with normal Hb (Hb-AA were selected as a control group.The average rates of hemoglobin, hematocrit, and red blood cells tended to be significantly lower in subjects with Hb-SS (p<0.001. The average rates of white blood cells, platelets, reticulocytes and serum LDH were significantly higher in subjects with Hb-SS (p<0.001. The average rates of Hb, HbF, hematocrit and red blood cells of Hb-SS patients with asymptomatic clinical phenotype were significantly higher than those of the two other phenotypes. However, the average rates of white blood cells, platelets, reticulocytes, and LDH of Hb-SS patients with the severe clinical phenotype are higher than those of two other clinical phenotypes. Significant correlations were observed between Hb and white blood cell in severe clinical phenotype (r3 = -0.37 * between Hb and red blood cells in the three phenotypes (r1 = 0.69 * r2 * = 0.69, r3 = 0.83 *, and finally between Hb and reticulocytes in the asymptomatic clinical phenotype and severe clinical phenotype (r1 = -0.50 * r3 = 0.45 *. A significant increase in LDH was observed in patients with leg ulcer, cholelithiasis and aseptic necrosis of the femoral head.The increase in serum LDH is accompanied by changes in hematological parameters. In our midst, serum LDH may be considered as an indicator of the severity of the disease.

  20. Correlation between the Lactate Dehydrogenase Levels with Laboratory Variables in the Clinical Severity of Sickle Cell Anemia in Congolese Patients

    Science.gov (United States)

    Mikobi, Tite Minga; Lukusa Tshilobo, Prosper; Aloni, Michel Ntetani; Mvumbi Lelo, Georges; Akilimali, Pierre Zalagile; Muyembe-Tamfum, Jean Jacques; Race, Valérie; Matthijs, Gert; Mbuyi Mwamba, Jean Marie

    2015-01-01

    Background Sickle cell anemia is an inflammatory disease and is characterized by chronic hemolysis. We sought to evaluate the association of lactate dehydrogenase levels with specific clinical phenotypes and laboratory variables in patients with sickle cell anemia. Methods The present cross-sectional study was conducted in Sickle Cell Centre of Yolo in Kinshasa, the Democratic Republic of Congo. Two hundred and eleven patients with Sickle Cell Anemia in steady state were recruited. Seventy-four participants with normal Hb (Hb-AA) were selected as a control group. Results The average rates of hemoglobin, hematocrit, and red blood cells tended to be significantly lower in subjects with Hb-SS (p<0.001). The average rates of white blood cells, platelets, reticulocytes and serum LDH were significantly higher in subjects with Hb-SS (p<0.001). The average rates of Hb, HbF, hematocrit and red blood cells of Hb-SS patients with asymptomatic clinical phenotype were significantly higher than those of the two other phenotypes. However, the average rates of white blood cells, platelets, reticulocytes, and LDH of Hb-SS patients with the severe clinical phenotype are higher than those of two other clinical phenotypes. Significant correlations were observed between Hb and white blood cell in severe clinical phenotype (r3 = -0.37 *) between Hb and red blood cells in the three phenotypes (r1 = 0.69 * r2 * = 0.69, r3 = 0.83 *), and finally between Hb and reticulocytes in the asymptomatic clinical phenotype and severe clinical phenotype (r1 = -0.50 * r3 = 0.45 *). A significant increase in LDH was observed in patients with leg ulcer, cholelithiasis and aseptic necrosis of the femoral head. Conclusion The increase in serum LDH is accompanied by changes in hematological parameters. In our midst, serum LDH may be considered as an indicator of the severity of the disease. PMID:25946088

  1. Cloning and characterization of HbMT2a, a metallothionein gene from Hevea brasiliensis Muell. Arg differently responds to abiotic stress and heavy metals

    Energy Technology Data Exchange (ETDEWEB)

    Li, Yan; Chen, Yue Yi; Yang, Shu Guang; Tian, Wei Min, E-mail: wmtian9110@126.com

    2015-05-22

    Metallothioneins (MTs) are of low molecular mass, cysteine-rich proteins. They play an important role in the detoxification of heavy metals and homeostasis of intracellular metal ions, and protecting against intracellular oxidative damages. In this study a full-length cDNA of type 2 plant metallothioneins, HbMT2a, was isolated from 25 mM Polyethyleneglycol (PEG) stressed leaves of Hevea brasiliensis by RACE. The HbMT2a was 372 bp in length and had a 237 bp open reading frame (ORF) encoding for a protein of 78 amino acid residues with molecular mass of 7.772 kDa. The expression of HbMT2a in the detached leaves of rubber tree clone RY7-33-97 was up-regulated by Me-JA, ABA, PEG, H{sub 2}O{sub 2}, Cu{sup 2+} and Zn{sup 2+}, but down-regulated by water. The role of HbMT2a protein in protecting against metal toxicity was demonstrated in vitro. PET-28a-HbMT2-beared Escherichia coli. Differential expression of HbMT2a upon treatment with 10 °C was observed in the detached leaves of rubber tree clone 93-114 which is cold-resistant and Reken501 which is cold-sensitive. The expression patterns of HbMT2a in the two rubber tree clones may be ascribed to a change in the level of endogenous H{sub 2}O{sub 2}. - Highlights: • Cloning an HbMT2a gene from rubber tree. • Analyzing expression patterns of HbMT2a upon abiotic stress and heavy metal stress. • Finding different expression patterns of HbMT2a among two Hevea germplasm. • The expressed protein of HbMT2a enhances copper and zinc tolerance in Escherichia coli.

  2. Food prices and consumer demand: differences across income levels and ethnic groups.

    Directory of Open Access Journals (Sweden)

    Cliona Ni Mhurchu

    Full Text Available BACKGROUND: Targeted food pricing policies may improve population diets. To assess their effects on inequalities, it is important to determine responsiveness to price changes across income levels and ethnic groups. OBJECTIVE: Our goal was to estimate price elasticity (PE values for major commonly consumed food groups in New Zealand, by income and ethnicity. PE values represent percentage change in demand associated with 1% change in price of that good (own-PE or another good (cross-PE. DESIGN: We used food expenditure data from national household economic surveys in 2007/08 and 2009/10 and Food Price Index data from 2007 and 2010. Adopting an Almost Ideal Demand System approach, own-PE and cross-PE estimates were derived for 24 food categories, household income quintiles, and two ethnic groups (Māori and non-Māori. RESULTS: Own-PE estimates (with two exceptions ranged from -0.44 to -1.78. Cross-PE estimates were generally small; only 31% of absolute values were greater than 0.10. Excluding the outlier 'energy drinks', nine of 23 food groups had significantly stronger own-PEs for the lowest versus highest income quintiles (average regression-based difference across food groups -0.30 (95% CI -0.62 to 0.02. Six own-PEs were significantly stronger among Māori; the average difference for Māori: non-Māori across food groups was -0.26 (95% CI -0.52 to 0.00. CONCLUSIONS: Food pricing policies have potential to improve population diets. The greater sensitivity of low-income households and Māori to price changes suggests the beneficial effects of such policies on health would be greatest for these groups.

  3. Food prices and consumer demand: differences across income levels and ethnic groups.

    Science.gov (United States)

    Ni Mhurchu, Cliona; Eyles, Helen; Schilling, Chris; Yang, Qing; Kaye-Blake, William; Genç, Murat; Blakely, Tony

    2013-01-01

    Targeted food pricing policies may improve population diets. To assess their effects on inequalities, it is important to determine responsiveness to price changes across income levels and ethnic groups. Our goal was to estimate price elasticity (PE) values for major commonly consumed food groups in New Zealand, by income and ethnicity. PE values represent percentage change in demand associated with 1% change in price of that good (own-PE) or another good (cross-PE). We used food expenditure data from national household economic surveys in 2007/08 and 2009/10 and Food Price Index data from 2007 and 2010. Adopting an Almost Ideal Demand System approach, own-PE and cross-PE estimates were derived for 24 food categories, household income quintiles, and two ethnic groups (Māori and non-Māori). Own-PE estimates (with two exceptions) ranged from -0.44 to -1.78. Cross-PE estimates were generally small; only 31% of absolute values were greater than 0.10. Excluding the outlier 'energy drinks', nine of 23 food groups had significantly stronger own-PEs for the lowest versus highest income quintiles (average regression-based difference across food groups -0.30 (95% CI -0.62 to 0.02)). Six own-PEs were significantly stronger among Māori; the average difference for Māori: non-Māori across food groups was -0.26 (95% CI -0.52 to 0.00). Food pricing policies have potential to improve population diets. The greater sensitivity of low-income households and Māori to price changes suggests the beneficial effects of such policies on health would be greatest for these groups.

  4. Haemoglobin J-Baltimore can be detected by HbA1c electropherogram but with underestimated HbA1c value.

    Science.gov (United States)

    Brunel, Valéry; Lahary, Agnčs; Chagraoui, Abdeslam; Thuillez, Christian

    2016-01-01

    Glycated haemoglobin (HbA(1c)) is considered the gold standard for assessing diabetes compensation and treatment. In addition, fortuitous detection of haemoglobin variants during HbA1c measurement is not rare. Recently, two publications reported different conclusions on accuracy of HbA(1c) value using capillary electrophoresis method in presence of haemoglobin J-Baltimore (HbJ).
Here we describe the fortuitous detection of unknown HbJ using capillary electrophoresis for measurement of HbA(1c). A patient followed for gestational diabetes in our laboratory presented unknown haemoglobin on Capillarys 2 Flex Piercing analyser which was identified as HbJ. HbJ is not associated with haematological abnormalities. High Performance Liquid Chromatography methods are known to possibly underestimate HbA(1c) value in the presence of this variant. This variant and its glycated form are clearly distinguished on electropherogram but HbJ was responsible for underestimating the true area of HbA(1c).
 Capillary electrophoresis is a good method for detecting HbJ but does not seem suitable for evaluation of HbA(1C) value in patients in presence of HbJ variant.

  5. A method comparison study between two hemoglobinometer models (Hemocue Hb 301 and Hb 201+) to measure hemoglobin concentrations and estimate anemia prevalence among women in Preah Vihear, Cambodia.

    Science.gov (United States)

    Rappaport, A I; Karakochuk, C D; Whitfield, K C; Kheang, K M; Green, T J

    2017-02-01

    Hemoglobin (Hb) concentration is often measured in global health and nutrition surveys to determine anemia prevalence using a portable hemoglobinometer such as the Hemocue® Hb 201+. More recently, a newer model was released (Hemocue Hb 301) utilizing slightly different methods to measure Hb as compared to the older model. The objective was to measure bias and concordance between Hb concentrations using the Hemocue Hb 301 and Hb 201+ models in a rural field setting. Hemoglobin (Hb) concentration was measured using one finger prick of blood (approximately 10 μL) from 175 Cambodian women (18-49 years) using three Hemocue Hb 201+ and three Hb 301 machines. Bias and concordance were measured and plotted. Overall, mean ± SD Hb concentration was 116 ± 13 g/L using the Hb 201+ and 118 ± 12 g/L using the Hb 301; and anemia prevalence (Hb < 120 g/L) was 58% (n = 102) and 58% (n = 101), respectively. Overall bias ± SD was 2.0 ± 10.5 g/L and concordance (95% CI) was 0.63 (0.54, 0.72). Despite the 2 g/L bias detected between models, anemia prevalence was very similar in both models. The two models measured anemia prevalence comparably in this population of women in rural Cambodia. © 2016 John Wiley & Sons Ltd.

  6. Comparison of Masking Level Difference in Patients with Multiple Sclerosis and Healthy Control Group

    Directory of Open Access Journals (Sweden)

    Soghrat Faghihzadeh

    2012-03-01

    Full Text Available Background and Aim: Multiple sclerosis (MS is a neurological disorder that involves central nervous system. Studies have showed that multiple sclerosis affects behavioral central auditory tests, such as masking release or masking level difference (MLD. The purpose of this study is to compare the masking level difference between multiple sclerosis patients and normal subjects.Methods: This cross sectional and non-interventional study was conducted on 32 multiple sclerosis patients aged between 20-50 years and 32 controls matched for age and gender in Faculty of Rehabilitation, Tehran University of Medical Sciences. masking level difference test was performed on each subject.Results: The mean masking level difference in the two groups was significantly different (p<0.01 however, gender did not prove to play a role in this difference.Conclusion: As part of the multiple sclerosis diagnosis panel, masking level difference test is an efficient modality for evaluation of hearing impairment and monitoring of rehabilitation progress.

  7. Discriminative Dictionary Learning With Two-Level Low Rank and Group Sparse Decomposition for Image Classification.

    Science.gov (United States)

    Wen, Zaidao; Hou, Biao; Jiao, Licheng

    2016-06-30

    Discriminative dictionary learning (DDL) framework has been widely used in image classification which aims to learn some class-specific feature vectors as well as a representative dictionary according to a set of labeled training samples. However, interclass similarities and intraclass variances among input samples and learned features will generally weaken the representability of dictionary and the discrimination of feature vectors so as to degrade the classification performance. Therefore, how to explicitly represent them becomes an important issue. In this paper, we present a novel DDL framework with two-level low rank and group sparse decomposition model. In the first level, we learn a class-shared and several class-specific dictionaries, where a low rank and a group sparse regularization are, respectively, imposed on the corresponding feature matrices. In the second level, the class-specific feature matrix will be further decomposed into a low rank and a sparse matrix so that intraclass variances can be separated to concentrate the corresponding feature vectors. Extensive experimental results demonstrate the effectiveness of our model. Compared with the other state-of-the-arts on several popular image databases, our model can achieve a competitive or better performance in terms of the classification accuracy.

  8. Measurement of HbA1c and HbA2 by Capillarys 2 Flex Piercing HbA1c programme for simultaneous management of diabetes and screening for thalassemia.

    Science.gov (United States)

    Ke, Peifeng; Liu, Jiawei; Chao, Yan; Wu, Xiaobin; Xiong, Yujuan; Lin, Li; Wan, Zemin; Wu, Xinzhong; Xu, Jianhua; Zhuang, Junhua; Huang, Xianzhang

    2017-10-01

    Thalassemia could interfere with some assays for haemoglobin A1c (HbA1c) measurement, therefore, it is useful to be able to screen for thalassemia while measuring HbA1c. We used Capillarys 2 Flex Piercing (Capillarys 2FP) HbA1c programme to simultaneously measure HbA1c and screen for thalassemia. Samples from 498 normal controls and 175 thalassemia patients were analysed by Capillarys 2FP HbA1c programme (Sebia, France). For method comparison, HbA1c was quantified by Premier Hb9210 (Trinity Biotech, Ireland) in 98 thalassaemia patients samples. For verification, HbA1c from eight thalassaemia patients was confirmed by IFCC reference method. Among 98 thalassaemia samples, Capillarys 2FP did not provide an HbA1c result in three samples with HbH due to the overlapping of HbBart's with HbA1c fraction; for the remaining 95 thalassaemia samples, Bland-Altman plot showed 0.00 ± 0.35% absolute bias between two systems, and a significant positive bias above 7% was observed only in two HbH samples. The HbA1c values obtained by Capillarys 2FP were consistent with the IFCC targets (relative bias below ± 6%) in all of the eight samples tested by both methods. For screening samples with alpha (α-) thalassaemia silent/trait or beta (β-) thalassemia trait, the optimal HbA2 cut-off values were ≤ 2.2% and > 2.8%, respectively. Our results demonstrated the Capillarys 2FP HbA1c system could report an accurate HbA1c value in thalassemia silent/trait, and HbA2 value (≤ 2.2% for α-thalassaemia silent/trait and > 2.8% for β-thalassemia trait) and abnormal bands (HbH and/or HbBart's for HbH disease, HbF for β-thalassemia) may provide valuable information for screening.

  9. 高血压人群中HbA1C诊断糖尿病的应用%High blood pressure in the diagnosis of diabetes HbA1C application

    Institute of Scientific and Technical Information of China (English)

    樊少勇

    2012-01-01

    Objective: To study the HbA1C test to hypertension patients diagnosis effectiveness of diabetes. Methods:888 cases of the need for the diagnosis of diabetes patients with hypertension were fasting blood glucose, OGTT (glucose tolerance experiment) and HbA1C test, and the statistical analysis. Results: The high blood pressure patients blood glucose level and the risk of diabetes, with its high blood pressure leels and rise; HbA1C can effective to hypertension patients with the diagnosis of diabetes, the diabetes used to the point of tangency value is 6.1%. Conclusion: HbA1C test to hypertension patients with diabetes diagnosis has good reliability, Diabetes HbA1C diagnosis the point of tangency value is 6.1%.%目的探讨HbA1C检测对高血压患者诊断糖尿病的有效性.方法对888例需要进行糖尿病诊断的高血压患者分别进行空腹血糖、OGTT(葡萄糖耐量实验)和HbA1C检测,并进行统计学分析.结果高血压患者的血糖水平和患糖尿病的风险随其高血压水平的升高而升高;HbA1C能有效的对高血压患者进行糖尿病的诊断,其用来糖尿病时的切点值为6.1%.结论 HbA1C检测对高血压患者的糖尿病诊断有良好的可靠性;HbA1C诊断糖尿病的切点值为6.1%.

  10. Group-level self-definition and self-investment: A hierarchical (multicomponent) model of in-group identification

    NARCIS (Netherlands)

    Leach, C.W.; van Zomeren, M.; Zebel, S.; Vliek, M.L.W.; Pennekamp, S.F.; Doosje, B.; Ouwerkerk, J.W.; Spears, R.

    2008-01-01

    Recent research shows individuals' identification with in-groups to be psychologically important and socially consequential. However, there is little agreement about how identification should be conceptualized or measured. On the basis of previous work, the authors identified 5 specific components

  11. Group-level self-definition and self-investment : A hierarchical (multicomponent) model of in-group identification

    NARCIS (Netherlands)

    Leach, Colin Wayne; van Zomeren, Martijn; Zebel, Sven; Vliek, Michael L. W.; Pennekamp, Sjoerd F.; Doosje, Bertjan; Ouwerkerk, Jaap W.; Spears, Russell

    Recent research shows individuals' identification with in-groups to be psychologically important and socially consequential. However, there is little agreement about how identification should be conceptualized or measured. On the basis of previous work, the authors identified 5 specific components

  12. Group-level self-definition and self-investment : A hierarchical (multicomponent) model of in-group identification

    NARCIS (Netherlands)

    Leach, Colin Wayne; van Zomeren, Martijn; Zebel, Sven; Vliek, Michael L. W.; Pennekamp, Sjoerd F.; Doosje, Bertjan; Ouwerkerk, Jaap W.; Spears, Russell

    2008-01-01

    Recent research shows individuals' identification with in-groups to be psychologically important and socially consequential. However, there is little agreement about how identification should be conceptualized or measured. On the basis of previous work, the authors identified 5 specific components o

  13. The Multi-Center Randomized Controlled Trial (RCT) Published by the Journal of the American Medical Association (JAMA) on the Effect of Periodontal Therapy on Glycated Hemoglobin (HbA1c) Has Fundamental Problems

    Science.gov (United States)

    Borgnakke, Wenche S.; Chapple, Iain L.C.; Genco, Robert J.; Armitage, Gary; Bartold, P. Mark; D’Aiuto, Francesco; Eke, Paul I.; Giannobile, William V.; Kocher, Thomas; Kornman, Kenneth S.; Lang, Niklaus P.; Madianos, Phoebus N.; Murakami, Shinya; Nishimura, Fusanori; Offenbacher, Steven; Preshaw, Philip M.; Rahman, Amin ur; Sanz, Mariano; Slots, Jørgen; Tonetti, Maurizio S.; Van Dyke, Thomas E.

    2015-01-01

    SUMMARY Subject Participants had type 2 diabetes, were on stable medication regimens, had HbA1c levels between 7% and <9%, retained at least 16 natural teeth, and had untreated chronic periodontitis. A total of 514 participants were enrolled between November 2009 and March 2012 from diabetes and dental clinics and communities affiliated with five participating academic medical centers. They were randomized with half (n = 257) allocated to a treatment group and the other half (n = 257) to a control group. Key Exposure/Study Factor The exposure was non-surgical periodontal treatment comprising scaling and root planing, oral hygiene instruction, and oral rinsing with chlorhexidine provided to the treatment group at baseline. Supportive periodontal therapy was also provided at 3 and 6 months. The control group received no treatment for the 6-month duration of the study. Main Outcome Measure The primary outcome measure was “the difference in change in HbA1c level from baseline between the two groups at 6 months.” Secondary outcomes included changes in periodontal probing depth (PPD), clinical attachment loss, bleeding on probing (BOP), gingival index, fasting glucose level, and Homeostasis Model Assessment (HOMA2) score. Main Results The authors report that enrollment into their Diabetes and Periodontal Therapy Trial (DPTT) was terminated early due to futility. At 6 months, mean HbA1c levels in the periodontal therapy group increased 0.17 (±1.0)%, compared with 0.11 (±1.0)% in the control group, with no significant difference between groups based on a linear regression model adjusting for clinical site (mean difference, −0.05% [95% CI: −0.23% to 0.12%]; p = 0.55). Periodontal measures improved in the treatment group compared with the control group at 6 months, with adjusted between-group differences of 0.28 mm (95% CI: 0.18–0.37) for PPD; 0.25 mm (95% CI: 0.14–0.36) for clinical attachment loss; 13.1% (95% CI: 8.1%–18.1%) for BOP; and 0.27 (95% CI: 0.17

  14. Efficacy of Thrice-daily versus Twice-daily Insulin Regimens on Glycohemoglobin (Hb A1c in Type 1 Diabetes Mellitus: A Randomized Controlled Trial

    Directory of Open Access Journals (Sweden)

    Zahra Razavi

    2011-01-01

    Full Text Available Objectives: To improve glycemic control and prevent late complications, the patient and diabetes team need to adjust insulin therapy. The aim of this study is to evaluate the efficacy of thrice-daily versus twice-daily insulin regimens on HbA1c for type 1 diabetes mellitus by a randomized controlled trial in Hamedan, west of Iran.Methods: The study included 125 patients under 19 years of age with type 1 diabetes mellitus over a 3-month period. All patients with glycohemoglobin (HbA1c ³8�0were followed prospectively and randomized into two trial and control groups. The control group received conventional two insulin injections per day: a mixture of short-acting (regular intermediated acting (NPH insulins pre-breakfast (twice daily, and the trial group was treated by an extra dose of regular insulin before lunch (three times daily. Main outcome measure was HbA1c at baseline and at the end of 3 months. The mean blood glucose level and number of hypoglycemia were recorded. All patients underwent monthly intervals follow up for assessing their home blood glucose records and insulin adjustment.Results: Overall, 100 patients completed the study protocol. 52�0were females, mean ±SD of age of 12.91 ± 3.9 years. There were no significant differences in baseline characteristics including age, gender, pubertal stage, adherence to diet, duration of disease and total daily insulin dose (p>0.05. There was a significant decrease individually in both groups in HbA1c level (p0.05.Conclusion: Compared with twice daily insulin, a therapeutic regimen involving the addition of one dose regular insulin before lunch caused no significant change in the overall glycemic control of patients with type 1 diabetes mellitus. Our results emphasize that further efforts for near normoglycemia should be focused upon education of patients in terms of frequent outpatient visits, more blood glucose monitoring and attention to insulin adjustments.

  15. The Multilevel Latent Covariate Model: A New, More Reliable Approach to Group-Level Effects in Contextual Studies

    Science.gov (United States)

    Ludtke, Oliver; Marsh, Herbert W.; Robitzsch, Alexander; Trautwein, Ulrich; Asparouhov, Tihomir; Muthen, Bengt

    2008-01-01

    In multilevel modeling (MLM), group-level (L2) characteristics are often measured by aggregating individual-level (L1) characteristics within each group so as to assess contextual effects (e.g., group-average effects of socioeconomic status, achievement, climate). Most previous applications have used a multilevel manifest covariate (MMC) approach,…

  16. Intensive management program to improve glycosylated hemoglobin levels and adherence to diet in patients with type 2 diabetes.

    Science.gov (United States)

    Song, Min-Sun; Kim, Hee-Seung

    2009-02-01

    This study investigated the effects of a diabetes outpatient intensive management program (DOIMP) on glycosylated hemoglobin (HbA(1)c) levels and adherence to diabetes control recommendations over a 12-week follow-up period for patients with diabetes. The DOIMP was composed of multidisciplinary diabetes education, complication monitoring, and telephone counseling. Twenty-five patients in the intervention group participated in the DOIMP, whereas 24 in the control group were briefed on the conventional description of diabetes mellitus by diabetes education nurses. Patients in the intervention group decreased their mean HbA(1)c levels by 2.3%, as compared with 0.4% in the control group. There was a significant increase in adherence to diet for the intervention group as compared with the control group. These findings indicate that the DOIMP can improve HbA(1)c levels and adherence to diet in patients with type 2 diabetes.

  17. Determinação de HbA1c por CLAE: interferência de variantes de hemoglobinas S e C e alta concentração de HbF HbA1c determination by HPLC: interference of hemoglobin variants HbS, HbC and HbF high concentration

    Directory of Open Access Journals (Sweden)

    Maria das Graças Santos Menezes

    2012-10-01

    Full Text Available INTRODUÇÃO: O diabetes mellitus (DM é considerado um problema importante de saúde pública; possui prevalência elevada e nos últimos anos observa-se aumento progressivo na sua incidência. OBJETIVO: verificar possíveis variações na concentração de hemoglobina (Hb glicada (HbA1c na presença de Hbs S e C e avaliar o impacto da redução da HbA1c na avaliação clínica e no monitoramento do paciente diabético. MATERIAL E MÉTODOS: Foram incluídos no estudo, 150 indivíduos diabéticos oriundos da cidade de Salvador, Bahia, de ambos os gêneros, com idade média de 56 anos. Foram determinadas a glicemia de jejum e a HbA1c por metodologia de oxidase-peroxidase e cromatografia líquida de alta eficiência (CLAE, respectivamente. RESULTADOS: Foram observadas variações na concentração da HbA1c em função da presença de variantes de Hb, como 7,85%, AA; 7,30%, AS e 7,15%, AC. DISCUSSÃO E CONCLUSÃO: A metodologia analítica a ser utilizada para determinação de HbA1c deve ser escolhida com base nas características gerais da população atendida e nas comorbidades associadas, pois a presença de Hbs S e C ocasiona reduções significativas de glicação. Essa redução pode levar a interpretações clínicas inadequadas relativas ao controle glicêmico dos pacientes.INTRODUCTION: Diabetes mellitus (DM is considered an important public health problem. It is highly prevalent and its incidence has progressively increased in recent years. OBJECTIVE: To verify possible variations of glycated hemoglobin (HbA1c concentration in the presence of Hb S and Hb C and to evaluate the impact of HbA1c reduction on clinical evaluation and monitoring of diabetic patients. MATERIAL AND METHODS: This study comprised 150 diabetic individuals from Salvador city, Bahia, from both genders and average age of 56 years old. Fast blood glucose and HbA1c were determined by oxidase-peroxidase and high-performance liquid chromatography (HPLC methods, respectively

  18. The History of GalaFLEX P4HB Scaffold

    Science.gov (United States)

    Williams, Simon F.; Martin, David P.; Moses, Arikha C.

    2016-01-01

    The GalaFLEX Scaffold (Galatea Surgical, Inc., Lexington, MA) for plastic and reconstructive surgery belongs to a new generation of products for soft tissue reinforcement made from poly-4-hydroxybutyrate (P4HB). Other members of this new family of products include MonoMax Suture (Aesculap AG, Tuttlingen, Germany) for soft tissue approximation, BioFiber Scaffold (Tornier, Inc., Edina, MN) for tendon repair, and Phasix Mesh (C.R. Bard, Inc., Murray Hill, NJ) for hernia repair. Each of these fully resorbable products provides prolonged strength retention, typically 50% to 70% strength retention at 12 weeks, and facilitates remodeling in vivo to provide a strong, lasting repair. P4HB belongs to a naturally occurring class of biopolymers and fibers made from it are uniquely strong, flexible, and biocompatible. GalaFLEX Scaffold is comprised of high-strength, resorbable P4HB monofilament fibers. It is a knitted macroporous scaffold intended to elevate, reinforce, and repair soft tissue. The scaffold acts as a lattice for new tissue growth, which is rapidly vascularized and becomes fully integrated with adjacent tissue as the fibers resorb. In this review, we describe the development of P4HB, its production, properties, safety, and biocompatibility of devices made from P4HB. Early clinical results and current clinical applications of products made from P4HB are also discussed. The results of post-market clinical studies evaluating the GalaFLEX Scaffold in rhytidectomy and cosmetic breast surgery demonstrate that the scaffold can reinforce lifted soft tissue, resulting in persistent surgical results in the face and neck at one year, and provide lower pole stability after breast lift at one year. PMID:27697885

  19. The History of GalaFLEX P4HB Scaffold.

    Science.gov (United States)

    Williams, Simon F; Martin, David P; Moses, Arikha C

    2016-11-01

    The GalaFLEX Scaffold (Galatea Surgical, Inc., Lexington, MA) for plastic and reconstructive surgery belongs to a new generation of products for soft tissue reinforcement made from poly-4-hydroxybutyrate (P4HB). Other members of this new family of products include MonoMax Suture (Aesculap AG, Tuttlingen, Germany) for soft tissue approximation, BioFiber Scaffold (Tornier, Inc., Edina, MN) for tendon repair, and Phasix Mesh (C.R. Bard, Inc., Murray Hill, NJ) for hernia repair. Each of these fully resorbable products provides prolonged strength retention, typically 50% to 70% strength retention at 12 weeks, and facilitates remodeling in vivo to provide a strong, lasting repair. P4HB belongs to a naturally occurring class of biopolymers and fibers made from it are uniquely strong, flexible, and biocompatible. GalaFLEX Scaffold is comprised of high-strength, resorbable P4HB monofilament fibers. It is a knitted macroporous scaffold intended to elevate, reinforce, and repair soft tissue. The scaffold acts as a lattice for new tissue growth, which is rapidly vascularized and becomes fully integrated with adjacent tissue as the fibers resorb. In this review, we describe the development of P4HB, its production, properties, safety, and biocompatibility of devices made from P4HB. Early clinical results and current clinical applications of products made from P4HB are also discussed. The results of post-market clinical studies evaluating the GalaFLEX Scaffold in rhytidectomy and cosmetic breast surgery demonstrate that the scaffold can reinforce lifted soft tissue, resulting in persistent surgical results in the face and neck at one year, and provide lower pole stability after breast lift at one year. © 2016 The American Society for Aesthetic Plastic Surgery, Inc.

  20. A simple method for HbF analysis.

    Science.gov (United States)

    von Mandach, U; Tuchschmid, P; Huch, A; Huch, R

    1987-01-01

    Spectrophotometric methods with CO-oxymeters for measurements of carboxyhemoglobin and/or oxygen saturation in human blood include a systematic error depending on the percentage of fetal hemoglobin. It is of clinical importance to estimate the fetal hemoglobin to correct HbCO and SO2 values respectively. The described method is simple and less time consuming than conventional methods like HPLC or electrophoresis. The two measurements of oxy- and carboxyhemoglobin in the same blood sample with different oxygen saturation are needed for the estimation of the HbF and can be performed, including the deoxygenation procedure, in about 40 minutes.

  1. FPG、HbA1c检测在重症颅脑外伤急性期病情评估及预后判断中的价值%Value of FPG detection and HbA1c detection in assessment and prognosis of severe traumatic brain injury at acute stage

    Institute of Scientific and Technical Information of China (English)

    范力

    2015-01-01

    目的探讨空腹血糖(fasting plasma glucose,FPG)与糖化血红蛋白(glycosylated hemoglobin A1c,HbA1c)检测在重症颅脑外伤急性期病情评估及预后判断中的应用价值. 方法 随机选取2012年12月~2014年12月期间我院收治的40例颅脑损伤患者,根据患者入院时的格拉斯哥昏迷指数评分将其分为重度颅脑外伤患者(观察组)和轻度颅脑外伤患者(对照组),每组各20例,分别对其进行空腹血糖(FPG)、糖化血红蛋白(HbA1c)的检测,对比分析两组患者的FPG水平和HbA1c水平与患者急性期病情评估及预后判断的关系. 结果 观察组的平均空腹血糖水平明显高于对照组,糖化血红蛋白水平较高的颅脑外伤患者病死率明显高于糖化血红蛋白水平低者(P<0.05). 结论 重度颅脑外伤患者急性期的空腹血糖及糖化血红蛋白水平是判断其颅脑损伤严重程度的重要参考指标,也为患者的预后判断提供了较准确的数据指标,具有良好的参考价值.%Objective To explore and analyze the application value of fasting blood glucose(FPG) detection and glyco-sylated hemoglobin A1c (HbA1c) detection in assessment and prognosis of severe traumatic brain injury at acute stage. Methods A total of 40 cases of patients with traumatic brain injury who were admitted to our hospital from December 2012 to December 2014, were randomly selected, according to the Glasgow Coma Scale score on admission, and they were divided into severe traumatic brain injury patients group and mild traumatic brain injury patients group, each group with 20 cases. Fasting plasma glucose(FPG) and glycosylated hemoglobin(HbA1c) were conducted in patients of the two groups respectively, and the relationship between FPG levels and HbA1c levels of patients in the two groups and assessment and prognosis of patients with severe traumatic brain injury at acute stage was compared and analyzed. Results The mean fasting plasma glucose levels(FPG) in patients

  2. RhHB1 mediates the antagonism of gibberellins to ABA and ethylene during rose (Rosa hybrida) petal senescence.

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    Lü, Peitao; Zhang, Changqing; Liu, Jitao; Liu, Xiaowei; Jiang, Guimei; Jiang, Xinqiang; Khan, Muhammad Ali; Wang, Liangsheng; Hong, Bo; Gao, Junping

    2014-05-01

    Rose (Rosa hybrida) is one of the most important ornamental plants worldwide; however, senescence of its petals terminates the ornamental value of the flower, resulting in major economic loss. It is known that the hormones abscisic acid (ABA) and ethylene promote petal senescence, while gibberellins (GAs) delay the process. However, the molecular mechanisms underlying the antagonistic effects amongst plant hormones during petal senescence are still unclear. Here we isolated RhHB1, a homeodomain-leucine zipper I transcription factor gene, from rose flowers. Quantitative RT-PCR and GUS reporter analyses showed that RhHB1 was strongly expressed in senescing petals, and its expression was induced by ABA or ethylene in petals. ABA or ethylene treatment clearly accelerated rose petal senescence, while application of the gibberellin GA3 delayed the process. However, silencing of RhHB1 delayed the ABA- or ethylene-mediated senescence, and resulted in higher petal anthocyanin levels and lower expression of RhSAG12. Moreover, treatment with paclobutrazol, an inhibitor of GA biosynthesis, repressed these delays. In addition, silencing of RhHB1 blocked the ABA- or ethylene-induced reduction in expression of the GA20 oxidase encoded by RhGA20ox1, a gene in the GA biosynthetic pathway. Furthermore, RhHB1 directly binds to the RhGA20ox1 promoter, and silencing of RhGA20ox1 promoted petal senescence. Eight senescence-related genes showed substantial differences in expression in petals after treatment with GA3 or paclobutrazol. These results suggest that RhHB1 mediates the antagonistic effect of GAs on ABA and ethylene during rose petal senescence, and that the promotion of petal senescence by ABA or ethylene operates through an RhHB1-RhGA20ox1 regulatory checkpoint.

  3. Correlation Between the Severity of Diabetic Peripheral Polyneuropathy and Glycosylated Hemoglobin Levels: A Quantitative Study

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    Lee, Won-Jae; Jang, Sol; Lee, Seung-Hwa

    2016-01-01

    Objective To investigate risk factors for diabetic peripheral polyneuropathy and their correlation with the quantified severity of nerve dysfunction in patients with diabetes mellitus (DM). Methods A total of 187 diabetic patients with clinically suspected polyneuropathy (PN) were subclassified into 2 groups according to electrodiagnostic testing: a DM-PN group of 153 diabetic patients without electrophysiological abnormality and a DM+PN group of 34 diabetic patients with polyneuropathy. For all patients, age, sex, height, weight, duration of DM, and plasma glycosylated hemoglobin (HbA1c) level were comparatively investigated. A composite score was introduced to quantitatively analyze the results of the nerve conduction studies. Logistic regression analysis and multiple regression analysis were used to evaluate correlations between significant risk factors and severity of diabetic polyneuropathy. Results The DM+PN group showed a significantly higher HbA1c level and composite score, as compared with the DM-PN group. Increased HbA1c level and old age were significant predictive factors for polyneuropathy in diabetic patients (odds ratio=5.233 and 4.745, respectively). In the multiple linear regression model, HbA1c and age showed a significant positive association with composite score, in order (β=1.560 and 0.253, respectively). Conclusion Increased HbA1c level indicative of a state of chronic hyperglycemia was a risk factor for polyneuropathy in diabetic patients and a quantitative measure of its severity. PMID:27152276

  4. Analyzing Group Level Effects with Clustered Data Using Taylor Series Linearization

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    Francis L. Huang

    2014-11-01

    Full Text Available Clustered data (e.g., students within schools are often analyzed in educational research where data are naturally nested. As a consequence, multilevel modeling (MLM has commonly been used to study the contextual or group-level (e.g., school effects on individual outcomes. The current study investigates the use of an alternative procedure to MLM: regression using Taylor series linearization (TSL variance estimation. Despite the name, regressions using TSL are straightforward to conduct, can yield consistent and unbiased estimates and standard errors (given the appropriate conditions, and can be performed using a variety of commercially- and freely-available statistical software. I analyze a subsample of the High School and Beyond (HSB dataset using MLM, regression using TSL, and ordinary least squares regression and compare results. In addition, 12,000 random samples are drawn from the HSB dataset of varying level-one and level-two sample sizes in order to compute biases in standard errors based on the different conditions. Sample R and SAS syntax showing how to run regressions using TSL are provided

  5. Gene-Silencing-Induced Changes in Carbohydrate Conformation in Relation to Bioenergy Value and Carbohydrate Subfractions in Modeled Plant (Medicago sativa) with Down-Regulation of HB12 and TT8 Transcription Factors.

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    Li, Xinxin; Hannoufa, Abdelali; Zhang, Yonggen; Yu, Peiqiang

    2016-05-13

    Gene silencing with RNA interference (RNAi) technology may be capable of modifying internal structure at a molecular level. This structural modification could affect biofunctions in terms of biodegradation, biochemical metabolism, and bioactive compound availability. The objectives of this study were to (1) Detect gene silencing-induced changes in carbohydrate molecular structure in an alfalfa forage (Medicago sativa spp. sativa: alfalfa) with down-regulation of genes that encode transcription factors TT8 and HB12; (2) Determine gene silencing-induced changes in nutrient bioutilization and bioavailability in the alfalfa forage (Medicago sativa); and (3) Quantify the correlation between gene silencing-induced molecular structure changes and the nutrient bioutilization and bioavailability in animals of ruminants. The experimental treatments included: T1 = Non-transgenic and no-gene silenced alfalfa forage (code "NT"); T2 = HB12-RNAi forage with HB12 gene down regulation (code "HB12"); T3 = TT8-RNAi forage with TT8 gene down regulation (code "TT8"). The HB12 and TT8 gene silencing-induced molecular structure changes were determined by non-invasive and non-destructive advanced molecular spectroscopy in a middle infrared radiation region that focused on structural, non-structural and total carbohydrate compounds. The nutrient bioutilization and bioavailability of the modified forage were determined using NRC-2001 system in terms of total digestive nutrient (TDN), truly digestible fiber (tdNDF), non-fiber carbohydrate (tdNDF), fatty acid (tdFA), crude protein (tdCP) and bioenergy profiles (digestible energy, metabolizable energy, net energy) for ruminants. The carbohydrate subfractions were evaluated using the updated CNCPS 6.0 system. The results showed that gene silencing significantly affected tdNFC (42.3 (NT) vs. 38.7 (HB12) vs. 37.4% Dry Matter (TT8); p = 0.016) and tdCP (20.8 (NT) vs. 19.4 (HB12) vs. 22.3% DM (TT8); p = 0.009). The gene-silencing also affected

  6. HbA1c Predicts Time to Diagnosis of Type 1 Diabetes in Children at Risk.

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    Helminen, Olli; Aspholm, Susanna; Pokka, Tytti; Hautakangas, Milla-Riikka; Haatanen, Nora; Lempainen, Johanna; Ilonen, Jorma; Simell, Olli; Knip, Mikael; Veijola, Riitta

    2015-05-01

    Prediction of type 1 diabetes is based on the detection of multiple islet autoantibodies in subjects who are at increased genetic risk. Prediction of the timing of diagnosis is challenging, however. We assessed the utility of HbA1c levels in predicting the clinical disease in genetically predisposed children with multiple autoantibodies. Cord blood samples from 168,055 newborn infants were screened for class II HLA genotypes in Finland, and 14,876 children with increased genetic risk for type 1 diabetes were invited to participate in regular follow-ups, including screening for diabetes-associated autoantibodies. When two or more autoantibodies were detected, HbA1c levels were analyzed at each visit. During follow-up, multiple (two or more) autoantibodies developed in 466 children; type 1 diabetes was diagnosed in 201 of these children (43%, progressors), while 265 children remained disease free (nonprogressors) by December 2011. A 10% increase in HbA1c levels in samples obtained 3-12 months apart predicted the diagnosis of clinical disease (hazard ratio [HR] 5.7 [95% CI 4.1-7.9]) after a median time of 1.1 years (interquartile range [IQR] 0.6-3.1 years) from the observed rise of HbA1c. If the HbA1c level was ≥5.9% (41 mmol/mol) in two consecutive samples, the median time to diagnosis was 0.9 years (IQR 0.3-1.5, HR 11.9 [95% CI 8.8-16.0]). In conclusion, HbA1c is a useful biochemical marker when predicting the time to diagnosis of type 1 diabetes in children with multiple autoantibodies.

  7. The evaluation of Tetanus-diphtheria (Td vaccine impacts on immune response to hepatitis B (HB vaccine in non-responder dialysis patientsFNx01

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    Shahrzad Shahidi

    2011-01-01

    Full Text Available Background: The Hepatitis B (HB vaccine response in hemodialysis patients is less than healthy individuals. Different strategies have been taken into account to improve the response rate. This study aimed to evaluate the effect of tetanus and diphtheria (Td vaccine as an adjuvant therapy to HB vaccination. Methods: Sixty three end-stage renal disease patients were recruited on dialysis that were older than 18 years and had passed at least 3 doses of HB vaccination schedule, and had HBS antibody (Ab with titer less than 10 IU/L. The patients were divided into two groups; A (30 patients and B (33 patients. Both of the groups received a 3-dose HB vaccination schedule of 40 μ g intramuscularly in the left deltoid muscle at 0, 1 and 6 months. Group A also received Td vaccine intramuscularly simultaneous with the first dose of HB vaccine. HBS Ab was measured in periods of 1 and 6 months after completion of the vaccination. Results: One month after completion of the vaccination, group A had better but not significant response rate (96% than group B (83.9% (p > 0.05; in addition, after 6 month there was no difference between the two groups (87.5% vs. 83.3% (p > 0.05. Patients with HCV infection had lower response rate than patients who did not have HCV infection (33.3% vs. 92.5% (p < 0.05. Age had negative effect on immune response to HB vaccination (r = -0.339; p = 0.005. Conclusions: The use of Td vaccine concurrent with HB vaccination may increase the response rate in non-responder individuals; however, it seems it does not have any role in the persistence of immune response. Age and HCV infection negatively affected the response to HB vaccination in dialysis patients.

  8. 2型糖尿病患者血清维生素D与糖化血红蛋白水平及胰岛素抵抗的相关性%The correlation of vitamin D, HbA1c and HOMA-IR in patients with type 2 diabetes mellitus

    Institute of Scientific and Technical Information of China (English)

    胡娟玉; 李礼

    2015-01-01

    Objective To discuss the relation of vitamin D with hemoglobin A1c (HbA1c) and HOMA-IR in patients with type 2 diabetes mellitus (T2DM). Methods 75 cases T2DM patients (research group) and 50 cases healthy people (control group) were collected in our hospital from March 2014 to August 2014. The fasting plasma glucose(FPG), fasting insulin(FIN), HbA1c, 25-hydroxy vitamin D3[25-(OH)D3] levels of all subjects were detected and HOMA-IR was calculation , all data were analyzed statistically. Results The levels of FPG, FIN, HbA1c and HOMA-IR in T2DM group were all higher than that of control group, and the differences all had statistical significance(Pall<0.05). The level of 25-(OH)D3 in T2DM group was lower than that of control group, and the difference had statistical significance (P<0.05). The level of 25-(OH)D3 had negative correlation with HbA1c and HOMA-IR(r=-0.548, r=-0.454, Pall<0.05). Conclusion The level of serum VitD has a remarkably decrease in T2DM patients , and has negative correlation with HbA1c level and HOMA-IR.%目的:探讨2型糖尿病(type 2 diabetes mellitus, T2DM)患者血清维生素 D(vitamin D, VitD)水平的变化与糖化血红蛋白A1c(hemoglobin A1c, HbA1c)及胰岛素抵抗的相关性。方法选取2014年3月至2014年8月来我院就诊的T2DM患者75例为研究组,同期健康体检者50例为对照组,检测两组中空腹血糖(fasting plasma glucose, FPG)、空腹胰岛素(fasting insulin, FIN)、HbA1c、25羟维生素D3[25-hydroxy vitamin D3,25-(OH)D3]水平并且计算胰岛素抵抗指数(HOMA-IR),对检测结果进行统计学分析。结果 T2DM组中FPG、FIN、HbA1c和HOMA-IR水平均高于对照组,差异均具有统计学意义(P均<0.05)。T2DM组血清25-(OH)D3水平显著低于对照组,差异具有统计学意义(P<0.05)。25-(OH)D3水平与HbA1c和HOMA-IR均呈负相关(r=-0.548,r=-0.454,P均<0.05)。结论2型糖

  9. Strategies for Sharing Scientific Research on Sea Level Rise: Suggestions from Stakeholder Focus Groups

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    DeLorme, D.; Hagen, S. C.; Stephens, S. H.

    2013-12-01

    This presentation reports results of focus groups with coastal resource managers on suggestions for effectively sharing sea level rise (SLR) scientific research with the public and other target audiences. The focus groups were conducted during three annual stakeholder workshops as an important and innovative component of an ongoing five-year multi-disciplinary NOAA-funded project, Ecological Effects of Sea Level Rise in the Northern Gulf of Mexico (EESLR-NGOM). The EESLR-NGOM project is assessing SLR risks to the natural and built environment along the Mississippi, Alabama, and Florida Panhandle coasts. The purpose was to engage stakeholders (e.g., coastal resource managers) in helping target, translate, and tailor the EESLR-NGOM project's scientific findings and emerging products so they are readily accessible, understandable, and useful. The focus groups provided insight into stakeholders' SLR informational and operational needs, solicited input on the project's products, and gathered suggestions for public communication and outreach. A total of three ninety-minute focus groups of between eight and thirteen participants each were conducted at annual workshops in Alabama, Florida, and Mississippi. The moderator asked a series of open-ended questions about SLR-related topics using an interview guide and encouraged participant interaction. All focus group audio-recordings were transcribed, and analyzed by carefully reading the 102 total pages of transcript data and identifying patterns and themes. Participants thought outreach about SLR impact and the EESLR-NGOM project scientific research/products was vital and acknowledged various communication challenges and opportunities. They identified three target audiences (local officials, general public, coastal resource managers themselves) that likely require different educational efforts and tools. Participants felt confident the EESLR-NGOM project products will benefit their resource planning and decision making and

  10. Individual and group level trajectories of behavioural development in Border collies

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    Riemer, Stefanie; Müller, Corsin; Virányi, Zsófia; Huber, Ludwig; Range, Friederike

    2017-01-01

    In order to assess dogs’ personality changes during ontogeny, a cohort of 69 Border collies was followed up from six to 18–24 months. When the dogs were 6, 12, and 18–24 months old, their owners repeatedly filled in a dog personality questionnaire (DPQ), which yielded five personality factors divided into fifteen facets. All five DPQ factors were highly correlated between the three age classes, indicating that the dogs’ personality remained consistent relative to other individuals. Nonetheless, at the group level significant changes with age were found for four of the five DPQ factors. Fearfulness, Aggression towards People, Responsiveness to Training and Aggression towards Animals increased with age; only Activity/Excitability did not change significantly over time. These changes in DPQ factor scores occurred mainly between the ages of 6 and 12 months, although some facets changed beyond this age. No sex differences were found for any of the tested factors or facets, suggesting that individual variation in personality was greater than male/female differences. There were significant litter effects for the factors Fearfulness, Aggression towards People and Activity/Excitability, indicating either a strong genetic basis for these traits or a high influence of the shared early environment. To conclude, from the age of six months, consistency in personality relative to other individuals can be observed in Border collies. However, at the group level, increases in fearful and aggressive behaviours occur up to 12 months and for some traits up to two years, highlighting the need for early interventions. Follow-up studies are needed to assess trajectories of personality development prior to six months and after two years, and to include a wider variety of breeds. PMID:28184101

  11. Comparison of the gold standard of hemoglobin measurement with the clinical standard (BGA) and noninvasive hemoglobin measurement (SpHb) in small children: a prospective diagnostic observational study.

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    Wittenmeier, Eva; Bellosevich, Sophia; Mauff, Susanne; Schmidtmann, Irene; Eli, Michael; Pestel, Gunther; Noppens, Ruediger R

    2015-10-01

    Collecting a blood sample is usually necessary to measure hemoglobin levels in children. Especially in small children, noninvasively measuring the hemoglobin level could be extraordinarily helpful, but its precision and accuracy in the clinical environment remain unclear. In this study, noninvasive hemoglobin measurement and blood gas analysis were compared to hemoglobin measurement in a clinical laboratory. In 60 healthy preoperative children (0.2-7.6 years old), hemoglobin was measured using a noninvasive method (SpHb; Radical-7 Pulse Co-Oximeter), a blood gas analyzer (clinical standard, BGAHb; ABL 800 Flex), and a laboratory hematology analyzer (reference method, labHb; Siemens Advia). Agreement between the results was assessed by Bland-Altman analysis and by determining the percentage of outliers. Sixty SpHb measurements, 60 labHb measurements, and 59 BGAHb measurements were evaluated. In 38% of the children, the location of the SpHb sensor had to be changed more than twice for the signal quality to be sufficient. The bias/limits of agreement between SpHb and labHb were -0.65/-3.4 to 2.1 g·dl(-1) . Forty-four percent of the SpHb values differed from the reference value by more than 1 g·dl(-1) . Age, difficulty of measurement, and the perfusion index (PI) had no influence on the accuracy of SpHb. The bias/limits of agreement between BGAHb and labHb were 1.14/-1.6 to 3.9 g·dl(-1) . Furthermore, 66% of the BGAHb values differed from the reference values by more than 1 g·dl(-1) . The absolute mean difference between SpHb and labHb (1.1 g·dl(-1) ) was smaller than the absolute mean difference between BGAHb and labHb (1.5 g·dl(-1) /P = 0.024). Noninvasive measurement of hemoglobin agrees more with the reference method than the measurement of hemoglobin using a blood gas analyzer. However, both methods can show clinically relevant differences from the reference method (ClinicalTrials.gov: NCT01693016). © 2015 John Wiley & Sons Ltd.

  12. Epidemiological differences of lower urinary tract symptoms among female subpopulations and group level interventions

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    Avasarala Atchuta Kameswararao

    2008-01-01

    Full Text Available Objectives: 1 To study the risk factor profiles of lower urinary tract symptoms (LUTS among adolescent girls, housewives and working women and its socioeconomic and quality of life losses. 2 To undertake risk factor modifications using the adolescent girls. Design and Setting: Cross-sectional descriptive study followed by educational intervention. Statistical Methods: Cluster sampling, Proportions, confidence intervals, Chi square and t-Tests and Logistic regression. Materials and Methods: House to house survey was done in two villages and one urban ward. Seventy-five housewives, 75 working women and 180 adolescent girls were asked about the risk factors and losses due to LUTS. Three teams of adolescent girls were utilized to bring about behavioral modifications. Impact was measured through user perspectives obtained from the participants. Results: Risk factors, social, economic and quality of life losses were different among the three female populations. Overall prevalence of LUTS among the three groups is 61(18.5%. Improper anal washing technique, malnutrition, presence of vaginal discharge, use of unsanitary menstrual pads, pinworm infestation and use of bad toilets were the significant causes among girls. Presence of sexually transmitted diseases was a contributing factor among housewives and working women. Prolonged sitting the posture was also contributing to LUTS among working women. Seventy-four per cent of beneficiaries expressed that intervention is useful. Conclusions: The causes for LUTS and their consequences were differing among the three female subpopulations. Specific group level interventions using trained girls were successful.

  13. Epidemiological investigations of aircrew: an occupational group with low-level cosmic radiation exposure.

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    Zeeb, Hajo; Hammer, Gaël P; Blettner, Maria

    2012-03-01

    Aircrew and passengers are exposed to low-level cosmic ionising radiation. Annual effective doses for flight crew have been estimated to be in the order of 2-5 mSv and can attain 75 mSv at career end. Epidemiological studies in this occupational group have been conducted over the last 15-20 years, usually with a focus on radiation-associated cancer. These studies are summarised in this note. Overall cancer risk was not elevated in most studies and subpopulations analysed, while malignant melanoma, other skin cancers and breast cancer in female aircrew have shown elevated incidence, with lesser risk elevations in terms of mortality. In some studies, including the large German cohort, brain cancer risk appears elevated. Cardiovascular mortality risks were generally very low. Dose information for pilots was usually derived from calculation procedures based on routine licence information, types of aircraft and routes/hours flown, but not on direct measurements. However, dose estimates have shown high validity when compared with measured values. No clear-cut dose-response patterns pointing to a higher risk for those with higher cumulative doses were found. Studies on other health outcomes have shown mixed results. Overall, aircrew are a highly selected group with many specific characteristics and exposures that might also influence cancers or other health outcomes. Radiation-associated health effects have not been clearly established in the studies available so far.

  14. Coupling between intrinsic prefrontal HbO2 and central EEG beta power oscillations in the resting brain.

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    Gert Pfurtscheller

    Full Text Available There is increasing interest in the intrinsic activity in the resting brain, especially that of ultraslow and slow oscillations. Using near-infrared spectroscopy (NIRS, electroencephalography (EEG, blood pressure (BP, respiration and heart rate recordings during 5 minutes of rest, combined with cross spectral and sliding cross correlation calculations, we identified a short-lasting coupling (duration [Formula: see text] s between prefrontal oxyhemoglobin (HbO2 in the frequency band between 0.07 and 0.13 Hz and central EEG alpha and/or beta power oscillations in 8 of the 9 subjects investigated. The HbO2 peaks preceded the EEG band power peaks by 3.7 s in 6 subjects, with moderate or no coupling between BP and HbO2 oscillations. HbO2 and EEG band power oscillations were approximately in phase with BP oscillations in the 2 subjects with an extremely high coupling (squared coherence [Formula: see text] between BP and HbO2 oscillation. No coupling was identified in one subject. These results indicate that slow precentral (deoxyhemoglobin concentration oscillations during awake rest can be temporarily coupled with EEG fluctuations in sensorimotor areas and modulate the excitability level in the brains' motor areas, respectively. Therefore, this provides support for the idea that resting state networks fluctuate with frequencies of between 0.01 and 0.1 Hz (Mantini et.al. PNAS 2007.

  15. Coupling between intrinsic prefrontal HbO2 and central EEG beta power oscillations in the resting brain.

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    Pfurtscheller, Gert; Daly, Ian; Bauernfeind, Günther; Müller-Putz, Gernot R

    2012-01-01

    There is increasing interest in the intrinsic activity in the resting brain, especially that of ultraslow and slow oscillations. Using near-infrared spectroscopy (NIRS), electroencephalography (EEG), blood pressure (BP), respiration and heart rate recordings during 5 minutes of rest, combined with cross spectral and sliding cross correlation calculations, we identified a short-lasting coupling (duration [Formula: see text] s) between prefrontal oxyhemoglobin (HbO2) in the frequency band between 0.07 and 0.13 Hz and central EEG alpha and/or beta power oscillations in 8 of the 9 subjects investigated. The HbO2 peaks preceded the EEG band power peaks by 3.7 s in 6 subjects, with moderate or no coupling between BP and HbO2 oscillations. HbO2 and EEG band power oscillations were approximately in phase with BP oscillations in the 2 subjects with an extremely high coupling (squared coherence [Formula: see text]) between BP and HbO2 oscillation. No coupling was identified in one subject. These results indicate that slow precentral (de)oxyhemoglobin concentration oscillations during awake rest can be temporarily coupled with EEG fluctuations in sensorimotor areas and modulate the excitability level in the brains' motor areas, respectively. Therefore, this provides support for the idea that resting state networks fluctuate with frequencies of between 0.01 and 0.1 Hz (Mantini et.al. PNAS 2007).

  16. Ingroup identification and group-level narcissism as predictors of U.S. citizens' attitudes and behavior toward Arab immigrants.

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    Lyons, Patricia A; Kenworthy, Jared B; Popan, Jason R

    2010-09-01

    In four studies, the authors explored factors contributing to negative attitudes and behavior toward Arab immigrants in the United States. In Study 1, Americans reported greater threat from Arabs, compared to other groups (e.g., Latino, Asian). In Study 2, they tested the effects of ingroup identification and group-level narcissism on attitudes toward Arab, Latino, Asian, and European immigrants. Identification interacted with group narcissism in predicting attitudes toward Arab (but not other) immigrants, such that identification predicted negative attitudes toward Arab immigrants only at mean and high levels of group narcissism. Study 3 explored the convergent and discriminant validity of the group narcissism construct. In Study 4, the authors added a behavioral dependent measure. Again, ingroup identification predicted negative behavior and attitudes toward an Arab immigrant group (but not comparison groups) only at mean and high levels of group narcissism. Theoretical and practical implications are discussed.

  17. Comparison of the Effect of Noise Levels on Stress Response in Two Different Operation Groups in an Orthopedic Surgery Room

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    Hasibe Baytan Yildiz

    2016-09-01

    Full Text Available Aim: The aim of this randomized, single-blinded study was to evaluate the effects of noise on hemodynamic and neuroendocrine stress response by measuring the level of noise in the surgery rooms of patients undergoing knee operations under neuroaxial anesthesia. Gerec ve Yontem: We compared patient responses from two groups of patients: those undergoing knee operations in a surgery room where the noise level (measured in decibels is high, and those undergoing meniscus operations in a surgery room with lower noise levels. The STAI, the State-Trait Anxiety Inventory (STAI-1, and the anxiety test (STAI-2wereperformed at preoperative and postoperative periods. 20 ml of blood sample was taken for basal, intraoperative 30th minute, and postoperative 1st hour measurements. Systolic, diastolic, and mean arterial blood pressures were found to be higher in the high noise level group. ACTH levels were increased during the early postoperative period and became normal during the late postoperative period in the high noise level group whereas ACTH levels were significantly decreased in the low-noise level group. Basal cortisol levels were significantly higher in the high noise level group. HCRP, an inflammatory response mediator was found to be decreased in both groups. Early and late blood glucose levels were significantly higher in the high noise group. There was a greater increase in early and late blood glucose levels in the high noise group. In the postoperative period, although the state-trait anxiety inventory (STAI-2 levels being higher in patients subject to noisier environment determines how people feel independent of the conditions and state they are in, this result made us consider that the noise the patients were subjected to in the intraoperative period may cause a stress response. Discussion: As a result we believe that standard noise levels should be achieved by reducing the factors causing high noise levels in the operating room. This will

  18. THE IMPACT OF IRON DEFICIENCY ON HBA2 LEVEL IN BETA THALASSEMIA MINOR IN SULAIMANI NORTHEASTERN IRAQ

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    Awaz Ahmed Kamal

    2015-11-01

    Full Text Available Background: HbA2 plays a key role in screening programs for beta thalassemia because a small increase in this fraction is the most important marker of beta thalassemia heterozygous carriers. The potential impact of coincident iron deficiency on HbA2 based identification of beta thalassemia minor is worrisome issue for screening laboratories, this is especially true for resource-constrained settings where iron deficiency is wide spread and molecular confirmatory tests for borderline HbA2 values may be unavailable. Objective: the aim of this study is to evaluate the effect of iron deficiency on HbA2 level in order to improve the detection of beta thalassemia trait with or without iron deficiency in our population. Materials and Method: In this study 145 individuals were enrolled including normal controls (50, beta thalassemia minor (50 and coincident beta thalassemia with iron deficiency cases (45. Complete blood count, serum iron, total iron binding capacity and HbA2 with HbF estimation were done for every individual. Result: The mean HbA2 level was (2.4± 0.4 in control, (5.2 ± 0.9 in beta thalassemia minor and 5.1± 0.9 in coincident iron deficiency with beta thalassemia minors. All hematological parameters were significantly lower in beta thalassemia minor and coincident iron deficiency with beta thalassemia minor in comparison to the control group .Mean HbA2 level did not show a significant difference in thalassemia minor (5.2±0.9 when compared with the mean HbA2 levels in coincidentiron deficiency with beta thalassemia minor. Conclusion: The presence of iron deficiency did not preclude the detection of beta thalassemia minor in our population

  19. Clinical validation of different echocardiographic motion pictures expert group-4 algorythms and compression levels for telemedicine.

    Science.gov (United States)

    Barbier, Paolo; Alimento, Marina; Berna, Giovanni; Cavoretto, Dario; Celeste, Fabrizio; Muratori, Manuela; Guazzi, Maurizio D

    2004-01-01

    Tele-echocardiography is not widely used because of lengthy transmission times when using standard Motion Pictures Expert Groups (MPEG)-2 lossy compression algorythms, unless expensive high bandwidth lines are used. We sought to validate the newer MPEG-4 algorythms to allow further reduction in echocardiographic motion video file size. Four cardiologists expert in echocardiography read blindly 165 randomized uncompressed and compressed 2D and color Doppler normal and pathologic motion images. One Digital Video and 3 MPEG-4 compression algorythms were tested, the latter at 3 decreasing compression quality levels (100%, 65% and 40%). Mean diagnostic and image quality scores were computed for each file and compared across the 3 compression levels using uncompressed files as controls. File dimensions decreased from a range of uncompressed 12-83 MB to MPEG-4 0.03-2.3 MB. All algorythms showed mean scores that were not significantly different from uncompressed source, except the MPEG-4 DivX algorythm at the highest selected compression (40%, p=.002). These data support the use of MPEG-4 compression to reduce echocardiographic motion image size for transmission purposes, allowing cost reduction through use of low bandwidth lines.

  20. Predicting Group-Level Outcome Variables from Variables Measured at the Individual Level: A Latent Variable Multilevel Model

    Science.gov (United States)

    Croon, Marcel A.; van Veldhoven, Marc J. P. M.

    2007-01-01

    In multilevel modeling, one often distinguishes between macro-micro and micro-macro situations. In a macro-micro multilevel situation, a dependent variable measured at the lower level is predicted or explained by variables measured at that lower or a higher level. In a micro-macro multilevel situation, a dependent variable defined at the higher…

  1. 红细胞与血红蛋白检测对甲状腺功能减退患者的诊断分析%Diagnostic Analysis of RBC and Hb Examination for Hypothyroidism Pa-tients

    Institute of Scientific and Technical Information of China (English)

    杨颖; 闫慧

    2015-01-01

    目的:探讨血红蛋白(Hb)和红细胞(RBC)在甲减患者中的诊断意义。方法选择2014年9月—2015年9月在该院进行健康体检的甲状腺功能正常的女性96例作为该研究对照组,同期选择该院诊断为甲减的女性患者94例作为研究对象,即为甲减组。采用全血细胞分析仪对两组进行全血细胞测定和血清甲状腺功能测定,比较两组受试者甲功,Hb,RBC的变化,分析两组间贫血患病率的差异。结果甲减组与对照组比较,Hb及RBC均明显降低,甲减患者TSH水平明显高于正常人群,差异有统计学意义(P<0.01), FT3和FT4水平低于对照组,差异有统计学意义。结论甲减常易引起贫血,全血细胞分析对甲减患者尤其是甲减性贫血患者有较高的辅助诊疗价值。%Objective To discuss diagnostic significance of RBC and Hb examination for Hypothyroidism patients. Methods 96 cases normal female carried out healthy physical examination in our hospital from 2014.09 to 2015.09 were selected as control group, 94 cases clinically diagnosed Hypothyroidism female patients were selected as hypothyroidism group at the same time. Using automated hematology analyzer to determine whole blood and serum thyroid function, comparing Hb, RBC and thyroid function between the two groups, and analyzing anemia prevalence of differences between the two groups. Re-sults Compared with control group, Hb and RBC of hypothyroidism group were significantly lower, TSH level of hypothy-roidism patients were obviously higher than normal, there are significant differences between(P<0.01). Conclusion Hypothy-roidism often cause anemia, complete blood analysis have high clinical auxiliary diagnosis value for hypothyroidism patients, especially hypothyroidism anemia patients Hypothyroidism group often easy cause anemia, complete blood analysis especial-ly in patients.

  2. Determination of filter pore size for use in HB line phase II production of plutonium oxide

    Energy Technology Data Exchange (ETDEWEB)

    Shehee, T. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Crowder, M. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Rudisill, T. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2014-08-01

    H-Canyon and HB-Line are tasked with the production of plutonium oxide (PuO2) from a feed of plutonium (Pu) metal. The PuO2 will provide feed material for the Mixed Oxide (MOX) Fuel Fabrication Facility. After dissolution of the Pu metal in H-Canyon, plans are to transfer the solution to HB-Line for purification by anion exchange. Anion exchange will be followed by plutonium(IV) oxalate precipitation, filtration, and calcination to form PuO2. The filtrate solutions, remaining after precipitation, contain low levels of Pu ions, oxalate ions, and may include solids. These solutions are transferred to H-Canyon for disposition. To mitigate the criticality concern of Pu solids in a Canyon tank, past processes have used oxalate destruction or have pre-filled the Canyon tank with a neutron poison. The installation of a filter on the process lines from the HB-Line filtrate tanks to H-Canyon Tank 9.6 is proposed to remove plutonium oxalate solids. This report describes SRNL’s efforts to determine the appropriate pore size for the filters needed to perform this function. Information provided in this report aids in developing the control strategies for solids in the process.

  3. Ordering Power of Separate versus Grouped True-False Tests: Interaction of Type of Test with Knowledge Levels of Examinees.

    Science.gov (United States)

    Hsu, Louis M.

    1979-01-01

    A comparison of the relative ordering power of separate and grouped-items true-false tests indicated that neither type of test was uniformly superior to the other across all levels of knowledge of examinees. Grouped-item tests were found superior for examinees with low levels of knowledge. (Author/CTM)

  4. Exploring Secondary Students' Epistemological Features Depending on the Evaluation Levels of the Group Model on Blood Circulation

    Science.gov (United States)

    Lee, Shinyoung; Kim, Heui-Baik

    2014-01-01

    The purpose of this study is to identify the epistemological features and model qualities depending on model evaluation levels and to explore the reasoning process behind high-level evaluation through small group interaction about blood circulation. Nine groups of three to four students in the eighth grade participated in the modeling practice.…

  5. Effects of a meditation program on verbal creative levels in a group of studens in late secondary education

    Directory of Open Access Journals (Sweden)

    Franco Justo, Clemente

    2009-12-01

    Full Text Available This study analyzes the effects of a meditation program on level of verbal creativity (fluency, flexibility and originality in a group of students in Spain’s two-year educational program for university preparation (Bachillerato. Participants formed two groups: a experimental group that participated in a meditation program, and a control group that did not take part in this intervention. Creativitylevels for the two groups were assessed using the verbal battery of the Torrance Tests of Creative Thinking, with significant improvement found in the experimental group as compared to the control group for the variables studied.

  6. Hb Wilde and Hb Patagonia: two novel elongated beta-globin variants causing dominant beta-thalassemia.

    Science.gov (United States)

    Scheps, Karen G; Hasenahuer, Marcia A; Parisi, Gustavo; Fornasari, María S; Pennesi, Sandra P; Erramouspe, Beatriz; Basack, Felisa N; Veber, Ernesto S; Aversa, Luis; Elena, Graciela; Varela, Viviana

    2015-06-01

    We describe here the molecular and hematological characteristics of novel frameshift mutations in exon 2 of the HBB gene (in heterozygous state) found in two Argentinean pediatric patients with dominant β-thalassemia-like features. In Hb Wilde, HBB:c.270_273delTGAG(p.Glu90Cysfs*67), we detected the deletion of the third base of the codon 89 (T) and the codon 90 (GAG), whereas in Hb Patagonia, HBB:c.296_297dupGT(p.Asp99Trpfs*59), the frameshift mutation was due to a duplication of a 'GT' dinucleotide after the second base of codon 98 (GTG). The Hb Patagonia and Hb Wilde mutations would result in elongated β-globin chains with modified C-terminal sequences and a total of 155 and 157 amino acids residues, respectively. Based on bioinformatics and structural analysis, as well as protein modeling, we predict that the elongated β-globins would affect the formation of the αβ dimers and their stability, which would further support the mechanism for the observed clinical features in both patients.

  7. Influence of glycemic control on the levels of subgingival periodontal pathogens in patients with generalized chronic periodontitis and type 2 diabetes

    Directory of Open Access Journals (Sweden)

    Tamires Szeremeske MIRANDA

    Full Text Available Abstract Objective This study evaluated the influence of glycemic control on the levels and frequency of subgingival periodontal pathogens in patients with type 2 diabetes mellitus (DM and generalized chronic periodontitis (ChP. Material and Methods Fifty-six patients with generalized ChP and type 2 DM were assigned according to the levels of glycated hemoglobin (HbA1c into one of the following groups: HbA1c<8% (n=28 or HbA1c≥8% (n=28. Three subgingival biofilm samples from sites with probing depth (PD<5 mm and three samples from sites with PD≥5 mm were analyzed by quantitative Polymerase Chain Reaction (PCR for the presence and levels of Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Eubacterium nodatum, Parvimona micra, Fusobacterium nucleatum ssp. and Prevotella intermedia. Results The mean counts of F. nucleatum ssp. were statistically significantly higher in the sites with PD≥5 mm of the HbA1c≥8% group (p<0.05. Frequencies of detection of T. forsythia, E. nodatum, P. micra and F. nucleatum ssp. were all higher in the sites with PD≥5 mm of the patients with HbA1c≥8%, compared with those of patients with HbA1c<8% (p<0.05. Frequency of detection of P. intermedia was higher in the sites with PD<5 mm of the patients with HbA1c≥8% than those of the patients with HbA1c<8% (p<0.05. Conclusions Poor glycemic control, as indicated by HbA1c≥8%, is associated with increased levels and frequencies of periodontal pathogens in the subgingival biofilm of subjects with type 2 DM and ChP.

  8. The relationship between HbA(1c) and fasting plasma glucose in patients with increased plasma liver enzyme measurements

    DEFF Research Database (Denmark)

    Christiansen, R; Rasmussen, L Melholt; Nybo, H;

    2012-01-01

    levels of increased liver enzyme concentrations. Methods:  Data from 10 065 patients with simultaneous measurement of HbA(1c) , venous fasting plasma glucose, alanine aminotransferase and γ-glutamyl transferase were extracted from our laboratory database. Correlations were investigated in four patient...

  9. Blood selenium levels and contribution of food groups to selenium intake in adolescent girls in Iceland

    Directory of Open Access Journals (Sweden)

    Ingibjorg Gunnarsdottir

    2012-08-01

    Full Text Available Background/objectives: Significant changes have been reported in dietary habits and food availability in Iceland that would be expected to compromise selenium intake and status, especially among young people. These include substantial decreases in the consumption of fish and milk, as well as the selenium content of imported wheat. The aim of this study was to assess selenium in the diet and whole blood of adolescent girls, as well as define the most important foods contributing to intake and blood concentrations of selenium. Design: The subjects were 96 randomly selected girls, aged 16–20, who answered a validated food frequency questionnaire (FFQ for dietary assessment. Selenium intake from each food group was calculated in µg/day. Blood samples were collected for measurement of whole blood selenium. Results: Mean dietary selenium was 51±25 µg/day. Milk/dairy products, including cheese, contributed 36±14% of total dietary selenium; fish 18±12%; and bread/cereal products 13±6%. Mean whole blood selenium was 117±12 µg/l (range 90–208; nearly 90% of subjects were above the optimal level of 100 µg/l. Fish and bread/cereal products were the only foods significantly correlated with selenium in blood (r=0.32; P = 0.002 and r=0.22; P = 0.04, respectively while no correlation was found with milk and dairy products in spite of their greater contribution to total selenium intake. Conclusion: In this population of Icelandic adolescent girls, selenium intake and status seem acceptable. Judging from associations between intake and blood levels, fish and cereals may be the most important contributors to blood selenium.

  10. The level of Vitamin B12 and hemoglobin in patients with recurrent aphthous stomatitis

    Directory of Open Access Journals (Sweden)

    Kamran Sari

    2016-01-01

    Full Text Available Background: Recurrent aphthous stomatitis (RAS is a chronic inflammatory disease of the oral cavity. Although many factors have been suggested as possible causes of RAS, its precise etiology is controversial. Vitamin B12 (vit B12 and iron deficiencies may cause RAS. Aims: We investigated the level of serum hemoglobin (Hb and vit B12 in patients with RAS and to compare them with healthy controls. Materials and Methods: This was a prospective, cross-sectional study. Patients who had had at least one episode of oral ulcerations per month since childhood were diagnosed as having RAS. Blood samples were drawn from the RAS group and control group for measuring blood Hb and vit B12 concentrations. Results: Exactly 195 patients (62 men and 133 women with RAS and 217 healthy controls (62 men and 155 women were enrolled in the study. The RAS group had significantly lower mean Hb levels than the control group (P < 0.001. No significant difference in the mean vit B12 level was demonstrated between the study group and controls. Around 58 (29.7% patients were diagnosed as Hb deficient in the RAS group and 21 (9.7% patients in controls. Vit B12 deficiency was diagnosed in 63 (32.3% individuals in the RAS group and 32 (14.7% individuals among controls. Conclusions: In our study, vit B12 and Hb deficiency were found higher in patients with RAS compared to the control group. Therefore, we suggested the investigation of the level of vit B12 and Hb in patients with RAS.

  11. Incorporation of serum carcinoembryonic antigen levels into the prognostic grouping system of colon cancer.

    Science.gov (United States)

    Ozawa, Heita; Kotake, Kenjiro; Hosaka, Miki; Hirata, Akira; Nakagawa, Yusuke; Fujita, Shin; Sugihara, Kenichi

    2017-06-01

    This study aimed to clarify the significance of preoperative serum carcinoembryonic antigen (CEA) on disease-free survival (DFS) in colon cancer and propose a new prognostic grouping system. A multiinstitutional retrospective cohort of 7296 colon cancer patients who underwent R0 surgery between 1997 and 2006 was analyzed. We stratified preoperative serum CEA values into three categories (C-stages): C0 (normal CEA), C1A (up to double the cutoff value), and C1B (more than double the cutoff value) and stratified each TNM stage by C-stage. Multivariate analyses using Cox regression models were used to analyze the significance of C-stage on 5-year DFS. CEA level was an independent factor affecting DFS; the 5-year DFS of patients with C0 and C1, as well as those with C1A and C1B, differed significantly (C0 84.6%, C1 69.8%, C1A 72.7%, and C1B 66.4%, P TNM staging system may facilitate decision making regarding the use of adjuvant chemotherapy in colon cancer patients.

  12. [Costs of appendicitis treatment by diagnosis-related groups in a third-level pediatric hospital].

    Science.gov (United States)

    Tlacuilo-Parra, Alberto; Hernández-Hernández, Araceli; Venegas-Dávalos, Martha; Gutiérrez-Hermosillo, Violeta; Guevara-Gutiérrez, Elizabeth; Ambriz-González, Gabriela

    2014-01-01

    Diagnosis-related groups (DRGs) are widely used in Europe. They allow performing comparisons in hospitals and incurrent hospital payment systems, defining the payment categories. We undertook this study to classify children who underwent appendectomy according to DRGs. Cross-sectional study. Comorbidities, length of hospitalization, histopathologic classification, and DRG classifications were analyzed. We included 313 patients, 62% males, with an average age of 8 ± 3 years; 91% were referred by another hospital and 67% were treated at night. Average length of hospitalization was 4 ± 3 days. There were comorbidities in 8% and surgical complications in 11%. According to histopathology, appendicitis was edematous (11%), suppurative (36%), gangrenous (22%), perforated (29%), and abscessed (2%). At discharge, 97% of the patients were healthy. Total cost for DRG 343 was $10,470,173.00 (Mexican pesos), DRG 342 was $1,227,592.00 and DRG 340 was $511,521.00. The global amount was $12,209,286.00 (Mexican pesos). The unitary cost for treatment of appendectomy for DRG 343 was $37,935.00, for DRG 342 was $49,103.00 and for DRG 340 was $42,626.00 (Mexican pesos). Because 88% of the cases of appendicitis were uncomplicated, this amount of money could be spent to treat these patients in a second-level hospital, using reimbursement 343 without generating additional expenses.

  13. Detecting the Subtle Shape Differences in Hemodynamic Responses at the Group Level

    Directory of Open Access Journals (Sweden)

    Gang eChen

    2015-10-01

    Full Text Available The nature of the hemodynamic response (HDR is still not fully understood due to the multifaceted processes involved. Aside from the overall amplitude, the response may vary across cognitive states, tasks, brain regions, and subjects with respect to characteristics such as rise and fall speed, peak duration, undershoot shape, and overall duration. Here we demonstrate that the fixed-shape or adjusted-shape methods may fail to detect some shape subtleties. In contrast, the estimated-shape method (ESM through multiple basis functions can provide the opportunity to identify some subtle shape differences and achieve higher statistical power at both individual and group levels. Previously, some dimension reduction approaches focused on the peak magnitude, or made inferences based on the area under the curve or interaction, which can lead to potential misidentifications. By adopting a generic framework of multivariate modeling (MVM, we showcase a hybrid approach that is validated by simulations and real data. Unlike the few analyses that were limited to main effect, two- or three-way interactions, we extend the approach to an inclusive platform that is more adaptable than the conventional GLM, achieving a practical equipoise among representation, false positive control, statistical power, and modeling flexibility.

  14. Heterotic compactifications with principal bundles for general groups and general levels

    CERN Document Server

    Distler, J

    2007-01-01

    We examine to what extent heterotic string worldsheets can describe arbitrary E8xE8 gauge fields. The traditional construction of heterotic strings builds each E8 via a Spin(16)/Z2 subgroup, typically realized as a current algebra by left-moving fermions, and as a result, only E8 gauge fields reducible to Spin(16)/Z2 gauge fields are directly realizable in standard constructions. However, there exist perturbatively consistent E8 gauge fields which can not be reduced to Spin(16)/Z2, and so cannot be described within standard heterotic worldsheet constructions. A natural question to then ask is whether there exists any (0,2) SCFT that can describe such E8 gauge fields. To answer this question, we first show how each ten-dimensional E8 partition function can be built up using other subgroups than Spin(16)/Z2, then construct ``fibered WZW models'' which allow us to explicitly couple current algebras for general groups and general levels to heterotic strings. This technology gives us a very general approach to han...

  15. A single amino acid substitution in the group 1 Trypanosoma brucei gambiense haptoglobin-hemoglobin receptor abolishes TLF-1 binding.

    Directory of Open Access Journals (Sweden)

    E DeJesus

    Full Text Available Critical to human innate immunity against African trypanosomes is a minor subclass of human high-density lipoproteins, termed Trypanosome Lytic Factor-1 (TLF-1. This primate-specific molecule binds to a haptoglobin-hemoglobin receptor (HpHbR on the surface of susceptible trypanosomes, initiating a lytic pathway. Group 1 Trypanosoma brucei gambiense causes human African Trypanosomiasis (HAT, escaping TLF-1 killing due to reduced uptake. Previously, we found that group 1 T. b. gambiense HpHbR (TbgHpHbR mRNA levels were greatly reduced and the gene contained substitutions within the open reading frame. Here we show that a single, highly conserved amino acid in the TbgHpHbR ablates high affinity TLF-1 binding and subsequent endocytosis, thus evading TLF-1 killing. In addition, we show that over-expression of TbgHpHbR failed to rescue TLF-1 susceptibility. These findings suggest that the single substitution present in the TbgHpHbR directly contributes to the reduced uptake and resistance to TLF-1 seen in these important human pathogens.

  16. 杭州地区部分人群糖化血红蛋白的调查研究%Study on the level of glycosylated hemoglobin in physical examination population in Hangzhou city

    Institute of Scientific and Technical Information of China (English)

    马小红; 庄爱周; 李萌; 万晓晨

    2012-01-01

    目的:研究杭州地区部分人群糖化血红蛋白(glycosylated hemoglobin,Hb)水平的分布情况.方法:收集2010年8月- 2011年8月在我院体检的4850例体检人员样本,应用全自动糖化血红蛋白分析仪检测HbA1(%)和HbA1c(%)水平,分别按性别、年龄、HbA1c参考值及2010年糖尿病诊断新标准对样本进行分组,并进行统计分析.结果:结果表明,HbA1(%)和HbA1c(%)在不同性别和年龄的人群中的分布有显著性差异.结论:杭州地区男性Hb总体水平均明显高于女性,且随着年龄的增长Hb水平增高,且根据2010年新指南筛查出疑似糖尿病者增多,为糖尿病的早期发现、早期治疗及减少并发症的发生提供了前提.%Objective:To investigate the distribution of glycosylated hemoglobin (Hb) level in Hangzhou population. Methods:4850 cases of physical examination individuals in our hospital from 2010 August to 2011 August were recruited into this stud-y. HbAl (% ) and HbAlc (% ) level were detected by the automatic glycosylated hemoglobin analyzer, and the results were conducted statistical analysis by sex, age, the reference value of HbAlc and the 2010 new criteria for diagnosis of diabetes melli-tus, separately. Results:The results show that the distribution of HbAl (% ) and HbAlc (% ) levels existed significant difference in different genders and age groups of Hangzhou population. Conclusion:The Hb level of male in Hangzhou were significantly higher than that of female, and the Hb level became higher as the age increased. According to new 2010 guidelines, the suspected diabetes increased, which has provided basis for early detection, early treatment of diabetes and reduce the incidence of diabetes complications.

  17. DNA sequence analysis proves Hb M-Milwaukee-2 is due to beta-globin gene codon 92 (CAC-->TAC), the presumed mutation of Hb M-Hyde Park and Hb M-Akita.

    Science.gov (United States)

    Hutt, P J; Pisciotta, A V; Fairbanks, V F; Thibodeau, S N; Green, M M

    1998-01-01

    Among the causes of congenital methemoglobinemia, Hb M-Milwaukee-2 was one of the earliest described, in a patient who also had Hb E trait. The structure of Hb M-Milwaukee-2 has been elusive. DNA sequence analysis, as here reported, proves that this hemoglobin variant is due to the mutation CAC-->TAC at codon 92 of the beta-globin gene, corresponding to the substitution of tyrosine for histidine. This mutation is identical with that presumed to be the cause of Hb M-Hyde Park and Hb M-Akita. In addition, the DNA mutation of Hb E, GAG-->AAG at codon 26, was confirmed in this case.

  18. Agreement between aggregate and individual-level measures of income and education: a comparison across three patient groups

    Directory of Open Access Journals (Sweden)

    Lynd Larry D

    2011-03-01

    Full Text Available Abstract Background The association between lower socioeconomic status and poorer health outcomes has been observed using both individual-level and aggregate-level measures of income and education. While both are predictive of health outcomes, previous research indicates poor agreement between individual-level and aggregate-level measures. The purpose of this study was to determine the level of agreement between aggregate-level and individual-level measures of income and education among three distinct patient groups, specifically asthma, diabetes, and rheumatoid patients. Methods Individual-level measures of annual household income and education were derived from three separate surveys conducted among patients with asthma (n = 359, diabetes (n = 281 and rheumatoid arthritis (n = 275. Aggregate-level measures of income and education were derived from the 2001 Canadian census, including both census tract-and dissemination area-level measures. Cross-tabulations of individual-level income by aggregate-level income were used to determine the percentage of income classifications in agreement. The kappa statistic (simple and weighted, Spearman's rank correlations, and intra-class correlation coefficient (ICC were also calculated. Individual-level and aggregate-level education was compared using Chi-Square tests within patient groups. Point biserial correlation coefficients between individual-level and aggregate-level education were computed. Results Individual-level income was poorly correlated with aggregate-level measures, which provided the worst estimations of income among patients in the lowest income category at the individual-level. Both aggregate-level measures were best at approximating individual-level income in patients with diabetes, in whom aggregate-level estimates were only significantly different from individual-level measures for patients in the lowest income category. Among asthma patients, the proportion of patients classified by

  19. Fractal multi-level organisation of human groups in a virtual world

    CERN Document Server

    Fuchs, Benedikt; Thurner, Stefan

    2014-01-01

    Humans are fundamentally social. They have progressively dominated their environment by the strength and creativity provided by and within their grouping. It is well recognised that human groups are highly structured, and the anthropological literature has loosely classified them according to their size and function, such as support cliques, sympathy groups, bands, cognitive groups, tribes, linguistic groups and so on. Recently, combining data on human grouping patterns in a comprehensive and systematic study, Zhou et al. identified a quantitative discrete hierarchy of group sizes with a preferred scaling ratio close to $3$, which was later confirmed for hunter-gatherer groups and for other mammalian societies. Using high precision large scale Internet-based social network data, we extend these early findings on a very large data set. We analyse the organisational structure of a complete, multi-relational, large social multiplex network of a human society consisting of about 400,000 odd players of a massive m...

  20. Markers of glycemic control in the mouse: comparisons of 6-h- and overnight-fasted blood glucoses to Hb A1c.

    Science.gov (United States)

    Han, Byoung Geun; Hao, Chuan-Ming; Tchekneva, Elena E; Wang, Ying-Ying; Lee, Chieh Allen; Ebrahim, Benyamin; Harris, Raymond C; Kern, Timothy S; Wasserman, David H; Breyer, Matthew D; Qi, Zhonghua

    2008-10-01

    The present studies examined the relationship between fasting blood glucose and Hb A(1c) in C57BL/6J, DBA/2J, and KK/HlJ mice with and without diabetes mellitus. Daily averaged blood glucose levels based on continuous glucose monitoring and effects of 6-h vs. overnight fasting on blood glucose were determined. Daily averaged blood glucose levels were highly correlated with Hb A(1c), as determined with a hand-held automated device using an immunodetection method. R(2) values were 0.90, 0.95, and 0.99 in KK/HIJ, C57BL/6J, and DBA/2J, respectively. Six-hour fasting blood glucose correlated more closely with the level of daily averaged blood glucose and with Hb A(1c) than did blood glucose following an overnight fast. To validate the immunoassay-determined Hb A(1c), we also measured total glycosylated hemoglobin using boronate HPLC. Hb A(1c) values correlated well with total glycosylated hemoglobin in all three strains but were relatively lower than total glycosylated hemoglobin in diabetic DBA/2J mice. These results show that 6-h fasting glucose provides a superior index of glycemic control and correlates more closely with Hb A(1c) than overnight-fasted blood glucose in these strains of mice.

  1. Pregnancy outcome in patients with sickle cell disease in the UK--a national cohort study comparing sickle cell anaemia (HbSS) with HbSC disease.

    Science.gov (United States)

    Oteng-Ntim, Eugene; Ayensah, Benjamin; Knight, Marian; Howard, Jo

    2015-04-01

    We describe the findings from a national study of maternal and fetal outcomes of pregnancy in women with sickle cell disease (SCD). Data were collected via the United Kingdom Obstetric Surveillance System between 1 February 2010 and 31 January 2011 from 109 women, of whom 51 (46·8%) had HbSS and 44 (40·4%) had HbSC. Data included antenatal, maternal and fetal outcomes. Comparisons were made between women with HbSS and HbSC. Incidence of complications were acute pain (57%), blood transfusion (26%), urinary tract infection (UTI; 12%) and critical care unit admission (23%) and these were all more common in women with HbSS than HbSC. There was no difference in the incidence of acute chest syndrome, hypertension and venous thromboembolism between HbSS and HbSC. Women with HbSS were more likely to deliver at complications in mothers with SCD, even in women with HbSC, which has previously been considered to have a more benign phenotype in pregnancy.

  2. Can sharing experiences in groups reduce the burden of living with diabetes, regardless of glycaemic control?

    DEFF Research Database (Denmark)

    Due-Christensen, Mette; Zoffmann, Vibeke; Hommel, Eva

    2012-01-01

    Aims To test whether patients with Type 1 diabetes would join support groups and benefit by improving psychosocial functioning, regardless of their HbA1c levels. Methods A pre-post test with follow-up after 6 and 12 months was conducted as a concurrent mixed-method study. The convenience sample...

  3. Diagnóstico laboratorial de hemoglobinas semelhantes à HbS Laboratorial diagnosis for hemoglobin like HbS

    Directory of Open Access Journals (Sweden)

    Paula Juliana Antoniazzo Zamaro

    2002-01-01

    Full Text Available A hemoglobina S (HbS está presente na população brasileira com prevalência variável, dependente dos grupos raciais formadores de cada região. A migração eletroforética em pH alcalino apresenta similaridade com outras hemoglobinas, e estudos complementares para sua correta caracterização são necessários. No presente estudo objetivamos traçar um fluxograma com as metodologias disponíveis para a caracterização da hemoglobina S e das hemoglobinas que apresentam migração semelhante em pH alcalino. No período de janeiro a junho de 2000, analisamos amostras de sangue com suspeita de hemoglobina S encaminhadas ao Laboratório de Hemoglobinas da Unesp. Caracterizamos diferentes mutantes e formas interativas com hemoglobina S, por procedimentos eletroforéticos, em variados pH, análises citológicas e testes bioquímicos específicos. Os procedimentos de análise aplicados resultaram em orientação fornecida aos laboratórios de rotina sobre como proceder no diagnóstico laboratorial destas alterações de hemoglobina. Desta forma contribuímos para um melhor conhecimento sobre a variabilidade genética das hemoglobinas em nossa população, auxiliando no acompanhamento clínico e no aconselhamento genético das hemoglobinopatias com fisiopatologia relacionada à alteração.The hemoglobin S is presented at Brazilian population with variable prevalence dependent of constitutive racial groups in each region. The electrophoretic migration on alkaline pH showed similarities with another hemoglobins. Complementary studies to the correct caracterization becomes necessary. At the present study we aimed make a fluxogram with the avaliable methodologies for the caracterization of hemoglobin S and hemoglobins that present similar migration on alkaline pH. During the period of January to June, 2000, we had analised blood samples with suspicious hemoglobin S sent to the Laboratory of Hemoglobins, Unesp. Were caracterized different mutants and

  4. INCREASED VASOOCCLUSIVE CRISIS IN “O” BLOOD GROUP SICKLE CELL DISEASE PATIENTS: ASSOCIATION WITH UNDERLYING THROMBOSPONDIN LEVELS.

    Directory of Open Access Journals (Sweden)

    M. Al Huneini

    2017-04-01

    Full Text Available Abstract: Objectives: To explore the incidence of vaso-occlusive crisis (VOC in Blood Group “O” sickle cell disease (SCD patients, and correlate it with the blood group and thrombospondin (TSP levels. Methods: In 89 consecutive SCD patients, blood samples were obtained for vWF antigen, collagen binding activity, blood group typing, C-reactive protein, variant hemoglobin analysis (HPLC, Serum TSP 1 and TSP 2 levels, complete blood counts, liver function tests, LDH and renal function tests during VOC episodes and in steady state conditions. Results: In the steady state SCD patients (n=72, “O” blood group patients (n=37 showed significantly higher median serum TSP 1 and TSP 2 levels than the non “O” blood group patients [n=35] [p <0.05, Mann-Whitney test], with an inverse relation between VWF:Ag, Factor VIII:C and TSP levels. Furthermore, the serum TSP 1 and TSP 2 levels were significantly higher in patients presenting with acute VOC [n=17], and in those with repeated VOC’s (group 1, n=16 especially amongst those patients with blood group “O” [p, <0.05, Mann-Whitney test]. Conclusions: The study shows that there was an inverse relation between TSP and vWF levels, in blood group “O” SCD patients with an upregulation of the TSP levels. Expectedly, during active VOC crisis, the TSP 1 and TSP 2 levels were significantly elevated.    Key Words: VOC; SCD; TSP; vWD; Blood groups

  5. Cell model for the study of receptor and regulatory functions of human proHB-EGF

    Directory of Open Access Journals (Sweden)

    N. V. Korotkevych

    2014-08-01

    Full Text Available Developing of new models and approaches, particularly with fluorescent techniques, for investigation of intracellular transport of proHB-EGF and its ligand-receptor complexes is strongly required. In order to create a model for studying proHB-EGF functions the genetic construction pEGFP-N1-proHB-EGF, encoding proHB-EGF-EGFP which is fluorescent-labeled form of proHB-EGF with enhanced green fluorescent protein EGFP in the cytoplasmic terminus of the molecule, was obtained. Eukaryotic cells expressing fusion protein proHB-EGF-EGFP on the cell surface were obtained by transfection with pEGFP-N1-proHB-EGF. Expressed in the Vero cells proHB-EGF-EGFP could bind fluorescent derivative of nontoxic receptor-binding subunit B of diphtheria toxin mCherry-SubB. After stimulation of transfected cells with TPA (12-O-Tetradecanoylphorbol-13-acetate, proHB-EGF-EGFP formed a fluorescentl-labeled C-terminal fragment of the molecule – CTF-EGFP. Thus, the obtained genetic construction pEGFP-N1-proHB-EGF could be helpful in visualization of molecules proHB-EGF and CTF in cells, may open new possibilities for the studying of their functions, such as receptor function of proHB-EGF for diphtheria toxin, intracellular translocation of CTF and provide possibilities for natural proHB-EGF ligands search.

  6. Variation in managing asthma: experience at the medical group level in California.

    Science.gov (United States)

    Legorreta, A P; Liu, X; Zaher, C A; Jatulis, D E

    2000-04-01

    To explore the degree of variation in the quality of asthma management among physician groups participating in a managed care network. Cross-sectional observation. The study population consisted of patients with moderate or severe asthma identified through a pharmacy database from a managed care plan in 1996. The patients were surveyed to obtain their assessments of asthma care, including components on quality of care, quality of service, and outcomes of care. We selected 47 physician groups that provided services for at least 35 asthma patients who responded to the survey. Variations in the outcome variables across physician groups were described by quartile, range, and histogram. Compliance with national guidelines varied among physician groups but was generally low. Physician group rates for patient use of steroid inhalers ranged from 10.7% to 45.5% and daily peak flow meter use ranged from 0% to 13.1%. Satisfaction ratings were higher, with overall satisfaction with the quality of asthma care ranging from 74.6% to 94.3%. Outcomes also showed considerable variation among groups. One-month absenteeism rates ranged from 32% to 61%, and 65.7% to 94.3% of respondents did not have an emergency room visit in the past year. The quality of asthma care and service varied significantly across physician groups. Such reports for different physician groups make evidence-based outcomes information directly available to patients and physician groups, help patients make informed healthcare decisions, and stimulate quality improvement efforts by physician groups.

  7. Maternal Hemoglobin Levels during Pregnancy and their Association with Birth Weight of Neonates

    Science.gov (United States)

    Moghaddam Tabrizi, F; Barjasteh, S

    2015-01-01

    Back ground Anemia in pregnancy is associated with increased rates of maternal and perinatal mortality, premature delivery, low birth weight, and other adverse outcomes Materials and Methods A prospective study was conducted on 1405 Iranian pregnant women who delivered during 2015. Blood was collected from all the subjects to measure the hemoglobin (Hb) during 16-19 weeks, 22-24 weeks, and 34-36 weeks of gestation. According to the level of hemoglobin, it is divided into 4 groups. Group 1; Hb > 10.1 gm/100ml (control group), Group 2; Hb= 8.1-10 gm/100ml (mild anemia) Group 3; Hb= 6.5-8 gm/100ml (moderate anemia) Group 4; Hb 10 g/dl), considered as normal gave birth to heavier and normal babies (3.3 kg). The severity of anemia during three trimesters was closely associated with birth weight of newborns. Conclusion The low hemoglobin values during three trimesters of pregnancy were associated with low birth weight in Iran. The anemia can lead to intra uterine growth retardation. PMID:26985354

  8. A combined approach for β-thalassemia based on gene therapy-mediated adult hemoglobin (HbA) production and fetal hemoglobin (HbF) induction.

    Science.gov (United States)

    Zuccato, Cristina; Breda, Laura; Salvatori, Francesca; Breveglieri, Giulia; Gardenghi, Sara; Bianchi, Nicoletta; Brognara, Eleonora; Lampronti, Ilaria; Borgatti, Monica; Rivella, Stefano; Gambari, Roberto

    2012-08-01

    Gene therapy might fall short in achieving a complete reversion of the β-thalassemic phenotype due to current limitations in vector design and myeloablative regimen. Following gene transfer, all or a large proportion of erythroid cells might express suboptimal levels of β-globin, impairing the therapeutic potential of the treatment. Our aim was to evaluate whether, in absence of complete reversion of the β-globin phenotype upon gene transfer, it is possible to use fetal hemoglobin induction to eliminate the residual α-globin aggregates and achieve normal levels of hemoglobin. Transgenic K562 cell lines and erythroid precursor cells from β(0)39-thalassemia patients were employed. Gene therapy was performed with the lentiviral vector T9W. Induction of fetal hemoglobin was obtained using mithramycin. Levels of mRNA and hemoglobins were determined by qRT-PCR and HPLC. First, we analyzed the effect of mithramycin on K562 transgenic cell lines harboring different copies of a lentiviral vector carrying the human β-globin gene, showing that γ-globin mRNA expression and HbF production can be induced in the presence of high levels of β-globin gene expression and HbA accumulation. We then treated erythroid progenitor cells from β-thalassemic patients with T9W, which expresses the human β-globin gene and mithramycin separately or in combination. When transduction with our lentiviral vector is insufficient to completely eliminate the unpaired α-globin chains, combination of β-globin gene transfer therapy together with fetal hemoglobin induction might be very efficacious to remove the excess of α-globin proteins in thalassemic erythroid progenitor cells.

  9. Toothbrushing, Blood Glucose and HbA1c: Findings from a Random Survey in Chinese Population.

    Science.gov (United States)

    Su, Lingyu; Liu, Wenzhao; Xie, Bingwu; Dou, Lei; Sun, Jun; Wan, Wenjuan; Fu, Xiaoming; Li, Guangyue; Huang, Jiao; Xu, Ling

    2016-07-07

    Both diabetes and periodontal disease are prevalent in China. Poor oral hygiene practice is the major cause of periodontal disease. An association between oral hygiene practice and blood glucose level was reported in individuals with diabetes, but not in the general population. We examined the association in a population-based random survey recruiting 2,105 adults without previously diagnosed diabetes in Chongqing city, China. Plasma glucose and hemoglobin A1c (HbA1c) were measured, and a 2-hour oral glucose tolerance test was conducted for each respondent. Self-reported toothbrushing frequency was used as a proxy for oral hygiene practice. In a linear model controlling for potential confounders (demographic characteristics, socio-economic status, lifestyle risk factors, BMI, dental visit frequency, etc.), urban residents who barely brushed their teeth had an increase of 0.50 (95% CI: 0.10-0.90) mmol/L in fasting plasma glucose, and an increase of 0.26% (0.04-0.47%) in HbA1c, relative to those brushing ≥twice daily; for rural residents, the effects were 0.26 (0.05-0.48) mmol/L in fasting plasma glucose and 0.20% (0.09-0.31%) in HbA1c. Individuals with better oral practice tended to have lower level of blood glucose and HbA1c. Establishing good oral health behavioral habits may be conducive to diabetes prevention and control in the general population.

  10. Proteasome inhibition induces both antioxidant and hb f responses in sickle cell disease via the nrf2 pathway.

    Science.gov (United States)

    Pullarkat, Vinod; Meng, Zhuo; Tahara, Stanley M; Johnson, Cage S; Kalra, Vijay K

    2014-01-01

    Oxidant stress is implicated in the manifestations of sickle cell disease including hemolysis and vascular occlusion. Strategies to induce antioxidant response as well as Hb F (α2γ2) have the potential to ameliorate the severity of sickle cell disease. Nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 or Nrf2) is a transcription factor that regulates antioxidant enzymes as well as γ-globin transcription. The Nrf2 in the cytoplasm is bound to its adapter protein Keap-1 that targets Nrf2 for proteasomal degradation, thereby preventing its nuclear translocation. We examined whether inhibiting the 26S proteasome using the clinically applicable proteasome inhibitors bortezomib and MLN 9708 would promote nuclear translocation of Nrf2, and thereby induce an antioxidant response and as well as Hb F in sickle cell disease. Proteasome inhibitors induced reactive oxygen species (ROS) and thereby increased Nrf2-dependent antioxidant enzyme transcripts, elevated cellular glutathione (GSH) levels and γ-globin transcripts as well as Hb F levels in the K562 cell line and also in erythroid burst forming units (BFU-E) generated from peripheral blood mononuclear cells of sickle cell disease patients. These responses were abolished by siRNA-mediated knockdown of Nrf2. Proteasome inhibitors, especially newer oral agents such as MLN9708 have the potential to be readily translated to clinical trials in sickle cell disease with the dual end points of antioxidant response and Hb F induction.

  11. Plasma high-mobility group box 1 levels predict mortality after ST-segment elevation myocardial infarction

    DEFF Research Database (Denmark)

    Sørensen, Morten V; Pedersen, Sune; Møgelvang, Rasmus

    2011-01-01

    We evaluated the potential association between plasma high-mobility group box 1 (HMGB1) levels and outcome in patients with ST-segment elevation myocardial infarction (STEMI) treated with primary percutaneous coronary intervention.......We evaluated the potential association between plasma high-mobility group box 1 (HMGB1) levels and outcome in patients with ST-segment elevation myocardial infarction (STEMI) treated with primary percutaneous coronary intervention....

  12. Glycated Hemoglobin Levels in Patients with Decompensated Cirrhosis

    Directory of Open Access Journals (Sweden)

    Jeffrey Nadelson

    2016-01-01

    Full Text Available Introduction. Aim of this study is to determine if HbA1c levels are a reliable predictor of glycemic control in patients with decompensated cirrhosis. Methods. 200 unique patients referred for liver transplantation at University of Tennessee/Methodist University Transplant Institute with a HbA1c result were included. Three glucose levels prior to the “measured” A1c (MA1c were input into an HbA1c calculator from the American Diabetes Association website to determine the “calculated” A1c (CA1c. The differences between MA1c and CA1c levels were computed. Patients were divided into three groups: group A, difference of 1.5. Results. 97 (49% patients had hemoglobin A1c of less than 5%. Discordance between calculated and measured HbA1c of >0.5% was seen in 47% (n=94. Higher level of discordance of greater than >1.5 was in 12% of patients (n=24. Hemoglobin was an independent predictor for higher discordance (odds ratio 0.77 95%, CI 0.60–0.99, and p value 0.04. HbA1c was an independent predictor of occurrence of HCC (OR 2.69 955, CI 1.38–5.43, and p value 0.008. Conclusion. HbA1c is not a reliable predictor of glycemic control in patients with decompensated cirrhosis, especially in those with severe anemia.

  13. A new hemoglobin variant found during Hb A1c measurement: Hb Hokusetsu [beta52(D3)Asp-->Gly].

    Science.gov (United States)

    Nakanishi, T; Miyazaki, A; Kishikawa, M; Shimizu, A; Kishida, O; Sumi, S; Tsubakio, T; Imai, K

    1998-07-01

    A new beta chain variant was accidentally found through the assay of Hb A1c in a diabetic patient. The variant was detected by polyacrylamide gel isoelectrofocusing and electrospray ionization mass spectrometry. For sequence determination, globin was cleaved with combination of trypsin and lysyl endopeptidase and analyzed by high performance liquid chromatography connected to electrospray ionization mass spectrometry. An abnormal betaT-5 peptide was found by reconstructed selected ion monitoring. The collision-induced dissociation spectrum of an ion derived from the abnormal betaT-5 peptide revealed a new substitution, [beta52(D3)Asp-->Gly], named Hb Hokusetsu. The sequence was confirmed with an automatic sequencer using peptides isolated by reversed phase high performance liquid chromatography. Amplification of the beta-globin exon 2 and nucleotide sequencing revealed a GAT-->GGT mutation in codon 52 corresponding to an Asp-->Gly replacement. Electrospray ionization mass spectrometry analysis of the hemolysate showed a reasonable value of 10.4% for glycated globin. The variant migrated as Hb S on isoelectrofocusing. Hematological analysis revealed normal parameters. The patient's hemolysate showed normal stability in the isopropanol test. Oxygen equilibrium studies on the patient's red blood cells and hemolysate showed no significant change in oxygen affinity or cooperativity.

  14. Developmental effect of the XmnI site on Ggamma-globin gene expression among newborn Hb F-Malta-I [Ggamma117(G19)His-->Arg, CAT-->CGT] heterozygotes and adult beta+ -Thalassemia homozygotes.

    Science.gov (United States)

    Pulis, Svetlana; Scerri, Christian A; Wismayer, Pierre Schembri; Galdies, Ruth; Wettinger, Stephanie Bezzina; Felice, Alex E

    2007-01-01

    Hb F-Malta-I [Ggamma117(19)His-->Arg, CAT-->CGT] is a stable and benign variant of Hb F found in 1.8% of Maltese newborn. We studied 120 Hb F-Malta-I heterozygotes and four Hb F-Malta-I homozygotes. The mean proportion of Ggamma-F-Malta-I in Hb F was 0.26 +/- 0.03 for the Hb F-Malta-I heterozygotes and 0.58 +/- 0.06 for the Hb F-Malta-I homozygotes. The Hb F-Malta-I allele was shown to occur on a background of the common Mediterranean haplotype Va [+ + - - - - - + + -]. Furthermore, the common Mediterranean haplotypes Va, IIIb [- + + + - + + + + -], I [+ + - - - - - + + +] and II [- + - + + - + + + +] accounted for most (66.2%) of the wild-type alleles among the tested Hb F-Malta-I heterozygotes. Different genotypes at the 5' epsilon HincII, Ggamma and Agamma HindIII, and 3'psibeta HincII sites (but not at the 5' Ggamma XmnI site) were found to be linked to significant variations in the proportion of Ggamma-F-Malta-I and Ggamma-globins in the Hb F of newborn Hb F-Malta-I heterozygotes. Moreover, the 5' Ggamma XmnI site was found to be associated with variations in Hb F and Ggamma-globin levels in a population of adult Maltese beta-thalassemia (thal) homozygotes. This implies that a determinant linked to the XmnI site which effects Ggamma-globin gene expression is active in anemic adults but not in normal infants.

  15. Group Assessment at First Year and Final Degree Level: A Comparative Evaluation

    Science.gov (United States)

    Plastow, N.; Spiliotopoulou, G.; Prior, S.

    2010-01-01

    Group projects are an established but debated pedagogical technique in higher education. The purpose of this study was to assess the appropriateness of combining individual and group marks in assessment. A mixed method design involving correlational and comparative elements was used. The sample included one cohort of students who completed a group…

  16. Fractal multi-level organisation of human groups in a virtual world

    Science.gov (United States)

    Fuchs, Benedikt; Sornette, Didier; Thurner, Stefan

    2014-10-01

    Humans are fundamentally social. They form societies which consist of hierarchically layered nested groups of various quality, size, and structure. The anthropologic literature has classified these groups as support cliques, sympathy groups, bands, cognitive groups, tribes, linguistic groups, and so on. Anthropologic data show that, on average, each group consists of approximately three subgroups. However, a general understanding of the structural dependence of groups at different layers is largely missing. We extend these early findings to a very large high-precision large-scale internet-based social network data. We analyse the organisational structure of a complete, multi-relational, large social multiplex network of a human society consisting of about 400,000 odd players of an open-ended massive multiplayer online game for which we know all about their various group memberships at different layers. Remarkably, the online players' society exhibits the same type of structured hierarchical layers as found in hunter-gatherer societies. Our findings suggest that the hierarchical organisation of human society is deeply nested in human psychology.

  17. Fractal multi-level organisation of human groups in a virtual world.

    Science.gov (United States)

    Fuchs, Benedikt; Sornette, Didier; Thurner, Stefan

    2014-10-06

    Humans are fundamentally social. They form societies which consist of hierarchically layered nested groups of various quality, size, and structure. The anthropologic literature has classified these groups as support cliques, sympathy groups, bands, cognitive groups, tribes, linguistic groups, and so on. Anthropologic data show that, on average, each group consists of approximately three subgroups. However, a general understanding of the structural dependence of groups at different layers is largely missing. We extend these early findings to a very large high-precision large-scale internet-based social network data. We analyse the organisational structure of a complete, multi-relational, large social multiplex network of a human society consisting of about 400,000 odd players of an open-ended massive multiplayer online game for which we know all about their various group memberships at different layers. Remarkably, the online players' society exhibits the same type of structured hierarchical layers as found in hunter-gatherer societies. Our findings suggest that the hierarchical organisation of human society is deeply nested in human psychology.

  18. Cloning and expression analysis ofHbWRKY75 gene in leaf fromHevea brasiliensis%橡胶树WRKY家族转录因子HbWRKY75基因的克隆及表达分析

    Institute of Scientific and Technical Information of China (English)

    陈晓丽; 闫栋; 孙丽娜; 曾日中; 杨礼富

    2016-01-01

    A novel WRKY gene was cloned by RT-PCR from the leaf tissue ofHevea brasiliensis (para rubber tree) and designated asHbWRKY75. The open reading frame (ORF) ofHbWRKY75 gene was 603 bp encoding a protein with 201 amino acid residues. The HbWRKY75 had a typical WRKY domain from amino acid 110 to 160 and belonged to the class II protein of the WRKY transcription factor family. The homology analysis re-vealed that HbWRKY75 shared amino acid sequence identities of 92%, 85%, 71%, 63% and 57% with the WRKY75 protein fromVitis vinifera, Zea mays,Elaeis guineensis,Nelumbo nucifera, andGossypium hirsutum. The expression pattern ofHbWRKY75 gene was investigated using real-time quantitative PCR, and the results demonstrated that the transcript level ofHbWRKY75 gene in leaves was signiifcantly higher than those in other parts of rubber trees.HbWRKY75 gene was differentially expressed in leaves with different developmental stag-es, and mostly expressed in the leaf at the late stage of senescence. The expression level ofHbWRKY75 gene in the young leaves greatly increased compared with those of the control samples of rubber trees treated with Ethephon, MeJA (methyl jasmonic acid), PEG (polyethylene glycol), H2O2, NaCl, low temperature and bark tapping (mechanical damage); but ABA treatment down-regulated the expression ofHbWRKY75. The presented data suggested that HbWRKY75 may be a transcription factor involved in the leaf senescence and the responses to phytohormones such as ethylene etc., and play a regulatory role in response to abiotic stresses such as NaCl and low temperature of rubber trees.%从巴西橡胶树无性系PR107的叶片组织中克隆了1个新的WRKY家族转录因子基因,命名为HbWRKY75,其ORF序列长为603 bp,编码201个氨基酸。生物信息学分析表明, HbWRKY75在N端第110~160氨基酸之间含有1个典型的WRKY结构域,属II类WRKY转录因子家族成员,与葡萄、大豆、油棕、陆地棉和拟南芥等同类基因

  19. 巴西橡胶树(Hevea brasiliensis)HbMlo7基因克隆与表达分析%Molecular cloning and characterization ofHbMlo7 gene in rubber tree (Hevea brasiliensis)

    Institute of Scientific and Technical Information of China (English)

    何海霞; 张宇; 王萌; 杨叶; 郑服丛

    2016-01-01

    Mlo基因是一种植物防卫相关基因,负调控植物白粉病抗性。为解析巴西橡胶树(Hevea brasiliensis)中Mlo基因的结构与功能,从橡胶树品种‘热研7-33-97’胶乳中扩增了一个Mlo cDNA序列,将其命名为HbMlo7。该基因编码499个氨基酸,分子量为56.85 kDa。蛋白结构分析发现,该蛋白含有Mlo保守结构域,有一个核定位信号和7个跨膜结构区域,无信号肽。通过qRT-PCR发现HbMlo7基因主要在橡胶树树皮中表达,其表达量在干旱、机械伤害和白粉菌侵染处理下显著上调。脱落酸(ABA)、乙烯利(ETH)、茉莉酸(JA)和过氧化氢(H2O2)均能诱导橡胶树叶片中HbMlo7基因显著上调表达。研究认为HbMlo7是植物Mlo家族成员,参与橡胶树的抗逆反应机制和植物激素信号传导途径。%Mildew resistance locus o(Mlo) is a defence-related gene in plant, which negatively regulates plant resistance to powdery mildew. To analyze the structure and function ofMlo gene inHevea brasiliensis, we cloned a cDNA sequence of Mlo from latex of the rubber tree clone ‘Reyan 7-33-97’. We deifned it asHbMlo7. HbMlo7 gene encoded 499 amino acids with a predicted molecular mass of 56.85 kDa. The protein structure analysis revealed that HbMLO7 has one conserved domain of Mlo, one nuclear localization signal (NLS), seven transmembrane domains, and no signal peptide. Gene expression analysis by quantitative and reverse transcrip-tion PCR (qRT-PCR) indicated thatHbMlo7expressed mainly in the bark of the rubber tree. The expression level ofHbMlo7 was induced by drought, wounding and powdery mildew infection. Abscisic acid (ABA), ethe-phon (ETH), jasmonic acid (JA) and hydrogen peroxide (H2O2) signiifcantly upregulatedHbMlo7 geneexpres-sion in rubber tree leaves. The results suggest thatHbMlo7 is one of the members ofMlo family, and involved in response mechanisms of stress and signal transduction pathway of plant hormones in rubber tree.

  20. Safety and immunogenicity of the DTP/HB /Hib combination vaccine: phase I study

    Directory of Open Access Journals (Sweden)

    Kusnandi Rusmil

    2013-11-01

    Full Text Available Background The World Health Organization (WHO has recommended the introduction of hepatitis B (HB and Haemophilus influenza type b (Hib vaccines into routine childhood vaccination programs. A new diptheria/tetanus/pertussis (DTP/hepatitis B/Hib pentavalent combination vaccine has been developed. Objective To evaluate the safety and immunogenicity of a new combination DTP/HB/Hib liquid vaccine in infants. Methods An open-label, uncontrolled, prospective intervention phase I study was conducted on 30 healthy infants aged 6−11 weeks. Each subject received 3 doses of DTP/HB/Hib vaccine, formulated by Bio Farma, 0.5 mL intramuscularly at the left anterolateral thigh region using a 25-gauge needle of 25 mm length. Subjects were followed for 1 month after administration of each vaccine dose to evaluate its safety, while serum anti-diphteria, tetanus, HBb, Hib, and pertussis antibodies were measured prior to the 1st dose and 1 month after the 3rd dose. Results Among 30 vaccinated subjects, 18 infants had fever within 24 hours after the first vaccination. Most cases of fever were mild in intensity and resolved within 24 hours. No other systemic or local reactions, or serious adverse events were observed in our subjects during the study. The immunogenicity results after 3rd vaccine dose showed that the geometric mean titer of the anti-polyribosylribitol phosphate (PRP antibody levels increased significantly from 0.0041μg/mL to 4.37 μg/mL after vaccination, and most infants had a fourfold or greater rise in antibody levels over their pre-injection levels. All subjects who received DTP/HB/Hib liquid vaccine had seroprotective antibodies against tetanus, diphtheria,a and hepatitis B, while 29/30 infants had seroprotective antibodies against pertussis. Conclusion This new diphtheria/tetanus/pertusis/hepatitis B/Hib combination vaccine has excellent safety profile and antibody responses in infants. These results encourage further clinical evaluation in

  1. HbA1c measurements from dried blood spots : validation and patient satisfaction

    NARCIS (Netherlands)

    Fokkema, Margaretha; Bakker, Andries J; de Boer, Fokje; Kooistra, Jeltsje; de Vries, Sifra; Wolthuis, Albert

    2009-01-01

    Background: This study evaluates HbA1c measurements from dried blood spots collected on filter paper and compares HbA1c from filter paper (capillary blood) with HbA1c measured in venous blood. Methods: Patient satisfaction was evaluated using a questionnaire. The performance with the filter paper me

  2. HbA1c variability is associated with increased mortality and earlier hospital admission in people with Type 1 diabetes.

    Science.gov (United States)

    Walker, G S; Cunningham, S G; Sainsbury, C A R; Jones, G C

    2017-08-19

    Despite evidence of morbidity, no evidence exists on the relationship between HbA1c variability and mortality in Type 1 diabetes. We performed an observational study to investigate whether the association between HbA1c variability and mortality exists in a population of people with Type 1 diabetes. As a secondary outcome, we compared onset of first hospital admission between groups. People with Type 1 diabetes were identified for inclusion from the Scottish Care Information - Diabetes data set. This database includes data of all people known to have diabetes who live within Scotland. A survival analysis was carried out over a 47-month period comparing two groups; group 1 with a HbA1c coefficient of variation (CV) above the median CV value, and group 2 with a CV below the median value. Time to death or first admission was also analysed. A Cox proportional hazard model was used to compare time to death, adjusting for appropriate covariables. Some 6048 individuals with Type 1 diabetes were included in the analysis. Median HbA1c CV was 7.9. The hazard ratio (HR) for mortality for those with an HbA1c CV above the median value is 1.5 over 47 months of follow-up (P < 0.001). HR for survival to either the first admission to hospital or death for those with an HbA1c CV above the median value was 1.35 (95% confidence interval 1.25-1.45) over 730 days of follow-up (P < 0.001). Our results show that people with greater HbA1c variability have a higher rate of mortality and earlier hospital admission in Type 1 diabetes. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

  3. Serum levels of lead and copper in a group of Egyptian children with ...

    African Journals Online (AJOL)

    Ehab

    the host immune system, and alter viral genome. There is increasing evidence ... the increased production of total immunoglobulin E. (IgE), which is also ... 1 included 30 asthmatic children during disease quiescence and group 2 included 15 ...

  4. Hemoglobin variants detected by hemoglobin A1c (HbA1c) analysis and the effects on HbA1c measurements

    OpenAIRE

    Nasir, Nadzimah Mohd; Thevarajah, M; Yean, Chew Yee

    2010-01-01

    Background: Hemoglobin (Hb) A1c is a tool widely used to monitor long-term glycemic control in diabetic patients. The objective of our study is to compare the HbA1c values measured on high performance liquid chromatography (HPLC) and immunoassay in patients who were detected to have hemoglobin variant after HbA1c analysis. Materials and Methods: We compared the HbA1c values measured using the Arkray Adams A1c HA-8160 (HPLC method) and Roche Cobas Integra (immunoturbidimetric method) from diab...

  5. Emergent group level navigation: an agent-based evaluation of movement patterns in a folivorous primate.

    Science.gov (United States)

    Bonnell, Tyler R; Campennì, Marco; Chapman, Colin A; Gogarten, Jan F; Reyna-Hurtado, Rafael A; Teichroeb, Julie A; Wasserman, Michael D; Sengupta, Raja

    2013-01-01

    The foraging activity of many organisms reveal strategic movement patterns, showing efficient use of spatially distributed resources. The underlying mechanisms behind these movement patterns, such as the use of spatial memory, are topics of considerable debate. To augment existing evidence of spatial memory use in primates, we generated movement patterns from simulated primate agents with simple sensory and behavioral capabilities. We developed agents representing various hypotheses of memory use, and compared the movement patterns of simulated groups to those of an observed group of red colobus monkeys (Procolobus rufomitratus), testing for: the effects of memory type (Euclidian or landmark based), amount of memory retention, and the effects of social rules in making foraging choices at the scale of the group (independent or leader led). Our results indicate that red colobus movement patterns fit best with simulated groups that have landmark based memory and a follow the leader foraging strategy. Comparisons between simulated agents revealed that social rules had the greatest impact on a group's step length, whereas the type of memory had the highest impact on a group's path tortuosity and cohesion. Using simulation studies as experimental trials to test theories of spatial memory use allows the development of insight into the behavioral mechanisms behind animal movement, developing case-specific results, as well as general results informing how changes to perception and behavior influence movement patterns.

  6. Emergent group level navigation: an agent-based evaluation of movement patterns in a folivorous primate.

    Directory of Open Access Journals (Sweden)

    Tyler R Bonnell

    Full Text Available The foraging activity of many organisms reveal strategic movement patterns, showing efficient use of spatially distributed resources. The underlying mechanisms behind these movement patterns, such as the use of spatial memory, are topics of considerable debate. To augment existing evidence of spatial memory use in primates, we generated movement patterns from simulated primate agents with simple sensory and behavioral capabilities. We developed agents representing various hypotheses of memory use, and compared the movement patterns of simulated groups to those of an observed group of red colobus monkeys (Procolobus rufomitratus, testing for: the effects of memory type (Euclidian or landmark based, amount of memory retention, and the effects of social rules in making foraging choices at the scale of the group (independent or leader led. Our results indicate that red colobus movement patterns fit best with simulated groups that have landmark based memory and a follow the leader foraging strategy. Comparisons between simulated agents revealed that social rules had the greatest impact on a group's step length, whereas the type of memory had the highest impact on a group's path tortuosity and cohesion. Using simulation studies as experimental trials to test theories of spatial memory use allows the development of insight into the behavioral mechanisms behind animal movement, developing case-specific results, as well as general results informing how changes to perception and behavior influence movement patterns.

  7. IAEA advisory group meeting on basic and applied problems of nuclear level densities

    Energy Technology Data Exchange (ETDEWEB)

    Bhat, M.R. (ed.)

    1983-06-01

    Separate entries were made in the data base for 17 of the 19 papers included. Two papers were previously included in the data base. Workshop reports are included on (1) nuclear level density theories and nuclear model reaction cross-section calculations and (2) extraction of nuclear level density information from experimental data. (WHK)

  8. Four-Group Classification Based on Fibrinogen Level and Fibrin Polymerization Associated With Postoperative Bleeding in Cardiac Surgery.

    Science.gov (United States)

    Kawashima, Shingo; Suzuki, Yuji; Sato, Tsunehisa; Kikura, Mutsuhito; Katoh, Takasumi; Sato, Shigehito

    2016-10-01

    Fibrinogen and fibrin formation have a key role in perioperative hemostasis. The aim of this study is to examine the association of postoperative hemostasis with a combined evaluation of the fibrinogen level and fibrin polymerization in cardiac surgery. We retrospectively classified 215 consecutive cardiac surgery patients into 4 groups (Fuji-san classification) that were divided by fibrinogen level fibrinogen thromboelastometry value at 10 minutes with rotational thromboelastometry <6 mm (ie, low fibrin polymerization) at the warming of cardiopulmonary bypass. Four groups resulted; group I, the acceptable range (n = 85); group II, only hypofibrinogenemia (<150 mg/dL, ≥6 mm, n = 63); group III, hypofibrinogenemia and low fibrin polymerization (<150 mg/dL, <6 mm, n = 60); and group IV, only low fibrin polymerization (≥150 mg/dL, <6 mm, n = 7). The risk of chest tube drainage volume greater than 500 mL within the first 24 hours after surgery (with group I as the reference) was increased in group II (odds ratio [OR], 3.3; 95% confidence interval [CI], 1.5-7.4; P < .01) and group III (OR, 8.5; 95% CI, 3.5-21.7; P < .01), and the risk greater than 1000 mL (with group I as the reference) was increased in group III (OR, 4.0; 95% CI, 1.1-17.3; P = .03) and group IV (OR, 23.1; 95% CI, 3.2-201.0; P < .01). Intraoperative blood transfusions were decreased by 24.5%, after stratifying the starting amount of fresh frozen plasma by the 4-group classification in the recent consecutive 65 (30.2%) patients (P < .01). The 4-group classification is associated with postoperative bleeding and may improve the quality of perioperative blood transfusion in cardiac surgery.

  9. Single-Use Disposable Electrochemical Label-Free Immunosensor for Detection of Glycated Hemoglobin (HbA1c) Using Differential Pulse Voltammetry (DPV).

    Science.gov (United States)

    Molazemhosseini, Alireza; Magagnin, Luca; Vena, Pasquale; Liu, Chung-Chiun

    2016-07-01

    A single-use disposable in vitro electrochemical immunosensor for the detection of HbA1c in undiluted human serum using differential pulse voltammetry (DPV) was developed. A three-electrode configuration electrochemical biosensor consisted of 10-nm-thin gold film working and counter electrodes and a thick-film printed Ag/AgCl reference electrode was fabricated on a polyethylene terephthalate (PET) substrate. Micro-fabrication techniques including sputtering vapor deposition and thick-film printing were used to fabricate the biosensor. This was a roll-to-roll cost-effective manufacturing process making the single-use disposable in vitro HbA1c biosensor a reality. Self-assembled monolayers of 3-Mercaptopropionic acid (MPA) were employed to covalently immobilize anti-HbA1c on the surface of gold electrodes. Electrochemical impedance spectroscopy (EIS) and X-ray photoelectron spectroscopy (XPS) confirmed the excellent coverage of MPA-SAM and the upward orientation of carboxylic groups. The hindering effect of HbA1c on the ferricyanide/ferrocyanide electron transfer reaction was exploited as the HbA1c detection mechanism. The biosensor showed a linear range of 7.5-20 µg/mL of HbA1c in 0.1 M PBS. Using undiluted human serum as the test medium, the biosensor presented an excellent linear behavior (R² = 0.999) in the range of 0.1-0.25 mg/mL of HbA1c. The potential application of this biosensor for in vitro measurement of HbA1c for diabetic management was demonstrated.

  10. Regulatory Effect of Estrogen, Progestin and HB-EGF on the Expression of HOXA10 Gene in Ishikawa Cells

    Institute of Scientific and Technical Information of China (English)

    LIU Xuemei; ZHU Guijin; ZHONG Gang

    2007-01-01

    HOXA10 gene plays an essential role in differentiation of the endometrium and in human reproduction. The aim of this study was to investigate the regulatory effect of sex steroids and HB-EGF on HOXA10 gene in Ishikawa cells. Ishikawa cells were incubated with 17-beta estradiol (10-8 mol/L), medroxyprogesterone acetate (MPA) (10-6 mol/L), RU486 (10-5 mol/L) or HB-EGF (10 ng/mL) for 48 h respectively. The expression of HOXA10 gene was detected by immunofluorescence,reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting. Our results showed that either estrogen alone, progestin alone or progestin combined with estrogen could significantly increase the expression of HOXA10 gene 48 h after the treatment (P<0.05). But estrogen combined with progestin and RU486 could inhibit the up-regulation by estrogen and progestin. HB-EGF could elevate the expression of HOXA10 gene 48 h after the treatment (P<0.05). It is concluded that both estrogen and progestin can up-regulate the expression of HOXA10 gene in Ishikawa cells, but RU486 can inhibit the effect and HB-EGF can elevate the expression level of HOXA10 gene.

  11. Variation in point-of-care testing of HbA1c in diabetes care general practice

    DEFF Research Database (Denmark)

    Kristensen, Troels

    2016-01-01

    Background and Aims Point-of-care testing (POCT) for HbA1c may result in improved diabetic control, better patient outcomes and enhanced clinical efficiency with fewer patient visits and subsequent reductions in costs. In 2008, the Danish regulators agreed to create a new fee for the remuneration...... of POCT of HbA1c in primary care. The aim of this study is to describe and analyze the variation in use of POCT of HbA1c among diabetes patients in Danish general practice and municipalities. Method We use register data from the year 2011 to define a population of 172,906 diabetes patients. The POCT fee...... is used to measure the amount of POCT of HbA1c among diabetes patients. Next we apply descriptive statistics to analyze variation in the prevalence of POCT versus laboratory testing at patient, clinic and municipality level. We include patient characteristics such as gender, age, socioeconomic markers...

  12. A multilevel analysis of the demands--control model: is stress at work determined by factors at the group level or the individual level?

    Science.gov (United States)

    Van Yperen, N W; Snijders, T A

    2000-01-01

    This study explored the extent to which negative health-related outcomes are associated with differences between work groups and with differences between individuals within work groups using R.A. Karasek's (1979) demands-control model. The sample consisted of 260 employees in 31 working groups of a national bank in the Netherlands. Results suggest that job demands and job control should be conceptualized as having both group- and individual-level foundations. Support for Karasek's demands-control model was found only when these variables were split into the 2 parts, reflecting shared perceptions and employees' subjective assessment, respectively. One of the most appealing practical implications is that absence rates among homogeneous work groups can be reduced by enhancing actual control on the job.

  13. The History of GalaFLEX P4HB Scaffold

    OpenAIRE

    Williams, Simon F.; Martin, David P; Moses, Arikha C.

    2016-01-01

    The GalaFLEX Scaffold (Galatea Surgical, Inc., Lexington, MA) for plastic and reconstructive surgery belongs to a new generation of products for soft tissue reinforcement made from poly-4-hydroxybutyrate (P4HB). Other members of this new family of products include MonoMax Suture (Aesculap AG, Tuttlingen, Germany) for soft tissue approximation, BioFiber Scaffold (Tornier, Inc., Edina, MN) for tendon repair, and Phasix Mesh (C.R. Bard, Inc., Murray Hill, NJ) for hernia repair. Each of these ful...

  14. HB29 LIS : Handbibliothek Library and Information Studies

    OpenAIRE

    Stiger, Birgit A.; Tinnacher, Judith; Perchtold, Günther

    2007-01-01

    In the course of the academic training for "Library and Information Studies" at the University Library Graz the project team dealt with the introduction of a reference library for "Library and Information Studies". Based on the old HB29 "Information Science" - which was largely out dated and not useable for the participants of the course – the team worked on lists of the books to be stored and those to be acquired including a budget plan. Moreover the team produced a new systematic for the ne...

  15. Assessing group-level participation in fluid teams: testing a new metric.

    Science.gov (United States)

    Paletz, Susannah B F; Schunn, Christian D

    2011-06-01

    Participation is an important factor in team success. We propose a new metric of participation equality that provides an unbiased estimate across groups of different sizes and across those that change size over time. Using 11 h of transcribed utterances from informal, fluid, colocated workgroup meetings, we compared the associations of this metric with coded equality of participation and standard deviation. While coded participation and our metric had similar patterns of findings, standard deviation had a somewhat different pattern, suggesting that it might lead to incorrect assessments with fluid teams. Exploratory analyses suggest that, as compared with mixed-age/status groups, groups of younger faculty had more equal participation and that the presence of negative affect words was associated with more dominated participation. Future research can take advantage of this new metric to further theory on team processes in both face-to-face and distributed settings.

  16. Behavioral suites mediate group-level foraging dynamics in communities of tropical stingless bees.

    Science.gov (United States)

    Lichtenberg, E M; Imperatriz-Fonseca, V L; Nieh, J C

    2010-02-01

    Competition for floral resources is a key force shaping pollinator communities, particularly among social bees. The ability of social bees to recruit nestmates for group foraging is hypothesized to be a major factor in their ability to dominate rich resources such as mass-flowering trees. We tested the role of group foraging in attaining dominance by stingless bees, eusocial tropical pollinators that exhibit high diversity in foraging strategies. We provide the first experimental evidence that meliponine group foraging strategies, large colony sizes and aggressive behavior form a suite of traits that enable colonies to improve dominance of rich resources. Using a diverse assemblage of Brazilian stingless bee species and an array of artificial "flowers" that provided a sucrose reward, we compared species' dominance and visitation under unrestricted foraging conditions and with experimental removal of group-foraging species. Dominance does not vary with individual body size, but rather with foraging group size. Species that recruit larger numbers of nestmates (Scaptotrigona aff. depilis, Trigona hyalinata, Trigona spinipes) dominated both numerically (high local abundance) and behaviorally (controlling feeders). Removal of group-foraging species increased feeding opportunities for solitary foragers (Frieseomelitta varia, Melipona quadrifasciata and Nannotrigona testaceicornis). Trigona hyalinata always dominated under unrestricted conditions. When this species was removed, T. spinipes or S. aff. depilis controlled feeders and limited visitation by solitary-foraging species. Because bee foraging patterns determine plant pollination success, understanding the forces that shape these patterns is crucial to ensuring pollination of both crops and natural areas in the face of current pollinator declines. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00040-009-0055-8) contains supplementary material, which is available to authorized

  17. Level of Education and Health Status of the Different Social Groups: Case Study Macedonia

    OpenAIRE

    Mirchevska, Lenche; Donev, Doncho; Mojsoska, Snezhana

    2008-01-01

    The educational level is an important indicator within the socioeconomical status for health evaluation and a powerful instrument in promotion of population’s health. In 2000 a study conducted in R. Macedonia, in 15 municipalities with different gross national product per capita, encompassing 1129 examinees older than 18 years. The results of the conducted research showed statistically significant association of the educational level with the morbidity. Higher morbidity emerges in persons tha...

  18. NGF and HB-EGF: potential biomarkers that reflect the effects of fesoterodine in patients with overactive bladder syndrome.

    Science.gov (United States)

    Kim, Soo Rim; Moon, Yeo Jung; Kim, Sei Kwang; Bai, Sang Wook

    2015-01-01

    To determine whether levels of nerve growth factor (NGF) and heparin-binding epidermal growth factor-like growth factor (HB-EGF) can be used to objectively assess overactive bladder syndrome (OAB) treatment outcome and to evaluate the effects of fixed-dose fesoterodine on OAB symptoms. This study included 124 participants (62 patients with OAB and 62 controls) in Severance Hospital between 2010 and 2012. In patients with OAB, 4 mg fesoterodine was administered once daily. Repeated evaluations of putative biomarker levels, urine creatinine (Cr) levels, and questionnaire responses, including the Overactive Bladder Symptom Score (OABSS) and the Overactive Bladder Questionnaire (OAB q), were performed from baseline to 16 weeks. Urinary levels of NGF/Cr (OAB: 1.13±0.9 pg/mg; control: 0.5±0.29 pg/mg) and HB-EGF/Cr (OAB: 8.73±6.55 pg/mg; control: 4.45±2.93 pg/mg) were significantly higher in subjects with OAB than in controls (pfesoterodine treatment, urinary NGF/Cr levels (baseline: 1.13±0.08 pg/mg; 16 weeks: 0.60±0.4 pg/mg; p=0.02) and HB-EGF/Cr levels significantly decreased (baseline: 8.73±6.55 pg/mg; 16 weeks: 4.72±2.69 pg/mg; p=0.03, respectively). Both the OABSS and OAB q scores improved (pfesoterodine improved OAB symptoms. Future studies are needed to further examine the significance of urinary NGF and HB-EGF levels as therapeutic markers for OAB.

  19. Postpartum hemorrhage is related to the hemoglobin levels at labor ...

    African Journals Online (AJOL)

    Kaima A. Frass

    2015-01-06

    Jan 6, 2015 ... tion (Hb < 10) during emergency cesarean delivery and to assess the Hb level at which .... hemoglobin and oxygen to uterus causing tissue enzymes and ... loon tamponade and systemic pelvic devascularization were.

  20. The interrelationships of glycemic control measures: HbA1c, glycated albumin, fructosamine, 1,5-anhydroglucitrol, and continuous glucose monitoring.

    Science.gov (United States)

    Beck, Roy; Steffes, Michael; Xing, Dongyuan; Ruedy, Katrina; Mauras, Nelly; Wilson, Darrell M; Kollman, Craig

    2011-12-01

    To describe the interrelationships of glycemic control measures: hemoglobin A1c (HbA1c), glycated albumin, fructosamine, 1,5-anhydroglucitrol (1,5-AG), and continuous glucose monitoring (CGM) in children and adolescents with type 1 diabetes. In total, 26 subjects of age 4-17 had HbA1c measurement followed within 14 d by three laboratory measures of glycemia and the collection of CGM glucose data (N = 21). Glycated albumin and fructosamine levels had a higher correlation with each other than with HbA1c. The correlation of 1,5-AG with HbA1c was lower (absolute r value = 0.25). All four measures had a similar degree of correlation with CGM-measured mean glucose (absolute r value = 0.50-0.56) and with hyperglycemic area under the curve (AUC) at 180 mg/dL (0.50-0.60). Each of the four measures (i.e., HbA1c, glycated albumin, fructosamine, and 1,5-AG) had a similar correlation with mean glucose and hyperglycemic AUC-180. 1,5-AG did not correlate with hyperglycemic AUC-180 better than did HbA1c. © 2011 John Wiley & Sons A/S.

  1. Simultaneous dual syringe electrospinning system using benign solvent to fabricate nanofibrous P(3HB-co-4HB)/collagen peptides construct as potential leave-on wound dressing.

    Science.gov (United States)

    Vigneswari, S; Murugaiyah, V; Kaur, G; Abdul Khalil, H P S; Amirul, A A

    2016-09-01

    The main focus of this study is the incorporation of collagen peptides to fabricate P(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] nano-fiber construct to further enhance surface wettability and support cell growth while harbouring desired properties for biodegradable wound dressing. Simultaneous electrospinning of nanofiber P(3HB-co-4HB)/collagen peptides construct was carried out using dual syringe system. The wettability of the constructs increased with the increase in 4HB molar fraction from 20mol% 4HB [53.2°], P(3HB-co-35mol%4HB)[48.9°], P(3HB-co-50mol%4HB)[44.5°] and P(3HB-co-82mol%4HB) [37.7°]. In vitro study carried out using mouse fibroblast cells (L929) grown on nanofiber P(3HB-co-4HB)/collagen peptides construct showed an increase in cell proliferation. In vivo study using animal model (Sprague Dawley rats) showed that nanofibrous P(3HB-co-4HB)/collagen peptides construct had a significant effect on wound contractions with the highest percentage of wound closure of 79%. Hence, P(3HB-co-4HB)/collagen peptides construct suitable for wound dressing have been developed using nano-fabrication technique.

  2. The "Gay Comfort Level": Examining a Media Advocacy Group's Efforts to Combat Youth Homophobia

    Science.gov (United States)

    Kachgal, Tara M.

    2011-01-01

    This article scrutinizes the efforts of a media advocacy group to redress the stigma of youth homosexuality among United States youth: a report published in 2003 by the Gay and Lesbian Alliance Against Defamation called, "How Youth Media Can Help Combat Homophobia Among American Teenagers." The report, authored by Rodger Streitmatter, concluded…

  3. The Science Advancement through Group Engagement Program: Leveling the Playing Field and Increasing Retention in Science

    Science.gov (United States)

    Hall, Donna M.; Curtin-Soydan, Amanda J.; Canelas, Dorian A.

    2014-01-01

    How can colleges and universities keep an open gateway to the science disciplines for the least experienced first-year science students while also maintaining high standards that challenge the students with the strongest possible high school backgrounds? The Science Advancement through Group Engagement (SAGE) project targets cohorts of less…

  4. Combat Air Forces Campaign Level Modernization Planning: A Study in Group Decision Making

    Science.gov (United States)

    2003-03-01

    problems of influence from dominant individuals, irrelevant conversation, and pressure for conformity . Individuals can influence and dominate the...to counteract 52 the negative effects of dominant personalities, conversation not pursuant to the problem, and open group pressure for conformity ...discussion and debates. However, during the observed process, there was no blatant evidence of pressure for conformity or the exertion of influence

  5. The "Gay Comfort Level": Examining a Media Advocacy Group's Efforts to Combat Youth Homophobia

    Science.gov (United States)

    Kachgal, Tara M.

    2011-01-01

    This article scrutinizes the efforts of a media advocacy group to redress the stigma of youth homosexuality among United States youth: a report published in 2003 by the Gay and Lesbian Alliance Against Defamation called, "How Youth Media Can Help Combat Homophobia Among American Teenagers." The report, authored by Rodger Streitmatter, concluded…

  6. A Phenomenological Exploration of the Experiences of Master's Level Counselor Trainees in Expressive Arts Group Supervision

    Science.gov (United States)

    Rossi, Martha Howe

    2010-01-01

    Expressive arts group supervision is the use of music, stories, movement, poetry or prose, role-play or psychodrama, art, guided imagery, or play to help trainees develop reflective skills (Wilkins, 1995), express thoughts and feelings (Knill, Levine & Levine, 2005; Lahad, 2000), develop new perspectives (Gladding, 2005), increase communication…

  7. Using Kirkpatrick's Four-Level-Evaluation Model To Explore the Effectiveness of Collaborative Online Group Work.

    Science.gov (United States)

    Baskin, Colin

    This paper examines two cohorts of students, each engaged in an undergraduate management course, but using different means of engagement. One cohort of 90 students completed a real time learning program integrating group dynamics, action research, team performance, and participative decision-making. A second cohort of 171 students completed the…

  8. Associations of group level popularity with observed behavior and influence in a dyadic context

    NARCIS (Netherlands)

    Lansu, T.A.M.; Cillessen, A.H.N.

    2015-01-01

    This study examined the association between popularity in the peer group and adolescents' behavior in a dyadic context. After collecting peer nominations for popularity, 218 early adolescents (Mage=11.0years) in 109 randomly composed same-sex dyads participated in a discussion task where they planne

  9. Use of Fructosyl Peptide Oxidase for HbA1c Assay

    OpenAIRE

    Yonehara, Satoshi; Inamura, Norio; Fukuda, Miho; Sugiyama, Koji

    2015-01-01

    ARKRAY, Inc developed the world’s first automatic glycohemoglobin analyzer based on HPLC (1981). After that, ARKRAY developed enzymatic HbA1c assay “CinQ HbA1c” with the spread and diversification of HbA1c measurement (2007). CinQ HbA1c is the kit of Clinical Chemistry Analyzer, which uses fructosyl peptide oxidase (FPOX) for a measurement reaction. This report mainly indicates the developmental background, measurement principle, and future of the enzymatic method HbA1c reagent.

  10. Use of fructosyl peptide oxidase for HbA1c assay.

    Science.gov (United States)

    Yonehara, Satoshi; Inamura, Norio; Fukuda, Miho; Sugiyama, Koji

    2015-03-01

    ARKRAY, Inc developed the world's first automatic glycohemoglobin analyzer based on HPLC (1981). After that, ARKRAY developed enzymatic HbA1c assay "CinQ HbA1c" with the spread and diversification of HbA1c measurement (2007). CinQ HbA1c is the kit of Clinical Chemistry Analyzer, which uses fructosyl peptide oxidase (FPOX) for a measurement reaction. This report mainly indicates the developmental background, measurement principle, and future of the enzymatic method HbA1c reagent.

  11. New HB-weighted time delay estimation algorithm under impulsive noise environment*

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    The traditional HB-weighted time-delay estimation (TDE) method degenerates under the impulsive noise environment. Two new time-delay estimation methods are proposed based on fractional lower order statistics (FLOS) according to the impulsive characteristics of fractional lower order α-stable noises. Theoretic analysis and computer simulations indicate that the proposed covariation based HB weighted (COV-HB) algorithm can suppress impulsive noises in one received signal for 1 ≤α≤ 2, whereas the other proposed fractional lower order covariance-based HB weighted (FLOC-HB) algorithm has robust performance under arbitrary impulsive noise conditions for the whole range of 0 < α≤ 2.

  12. Measuring Hemoglobin Levels in the Optic Disc of Parkinson's Disease Patients Using New Colorimetric Analysis Software

    Science.gov (United States)

    Bambo, Maria Pilar; Garcia-Martin, Elena; Satue, Maria; Perez-Olivan, Susana; Alayon, Silvia; Gonzalez-Hernandez, Marta; Polo, Vicente; Larrosa, Jose Manuel; Gonzalez-De la Rosa, Manuel

    2014-01-01

    Objective. To evaluate a new method of measuring hemoglobin (Hb) levels and quantifying the color changes in the optic nerve head of Parkinson's disease (PD) patients. We also compared differences in retinal nerve fiber layer (RNFL) thicknesses obtained using spectral domain optical coherence tomography (OCT) device between PD group and healthy group. Methods. One hundred and fifty-five PD patients and 91 sex- and age-matched healthy subjects were included in this cross-sectional study. OCT examinations and one photograph of the optic disc were performed. The Laguna ONhE (“optic nerve hemoglobin”; Insoft SL, Tenerife, Spain) software was used to analyze the Hb level on the acquired optic disc photographs. Results. PD patients exhibited significantly reduced mean optic disc Hb percentages (57.56% in PD, 67.63% in healthy subjects; P = 0.001) as well as reduced Hb in almost all analyzed sectors, with the largest differences detected in the inferior and nasal sectors. RNFL parameters were significantly reduced in PD patients compared with healthy subjects, especially in the inferior quadrant. Conclusions. Measurements of optic disc Hb levels obtained with the Laguna ONhE software had good ability to detect optic nerve color changes (more papillary paleness and consequently this could suggest optic atrophy and axonal loss) in PD patients. PMID:25587487

  13. Genetic analysis of lipolytic activities in Thermus thermophilus HB27.

    Science.gov (United States)

    Leis, Benedikt; Angelov, Angel; Li, Haijuan; Liebl, Wolfgang

    2014-12-10

    The extremely thermophilic bacterium Thermus thermophilus HB27 displays lipolytic activity for the hydrolysis of triglycerides. In this study we performed a mutational in vivo analysis of esterases and lipases that confer growth on tributyrin. We interrupted 10 ORFs suspected to encode lipolytic enzymes. Two chromosomal loci were identified that resulted in reduced hydrolysis capabilities against tributyrin and various para-nitrophenyl acyl esters. By implementation of a convenient new one-step method which abstains from the use of selectable markers, a mutant strain with multiple scar-less deletions was constructed by sequentially deleting ORFs TT_C1787, TT_C0340, TT_C0341 and TT_C0904. The quadruple deletion mutant of T. thermophilus exhibited significantly lower lipolytic activity (approximately 25% residual activity compared to wild type strain) over a broad range of fatty acyl esters and had lost the ability to grow on agar plates containing tributyrin as the sole carbon source. Furthermore, we were able to determine the impact of each gene disruption on the lipolytic activity profile in this model organism and show that the esterase activity in T. thermophilus HB27 is due to a concerted action of several hydrolases having different substrate preferences and activities. The esterase-less T. thermophilus multi-deletion mutant from this study can be used as a screening and expression host for esterase genes from thermophiles or metagenomes.

  14. The relationship between personality traits and anxiety/depression levels in different drug abusers' groups

    OpenAIRE

    2013-01-01

    AIM: Since psychosocial characteristics of drug abuse involve mainly specific personality and emotional changes, it is very important to investigate characteristics of addictive personality in relationship with emotional state of the individual. Considering that, the objective of this study was to analyse the relationship between personality structure and emotional state of two different groups: heroin addicts and recreate drug abusers. METHODS: The total of 288 (219 males and 69 females; 191...

  15. Functional group based Ligand binding affinity scoring function at atomic environmental level

    Science.gov (United States)

    Varadwaj, Pritish Kumar; Lahiri, Tapobrata

    2009-01-01

    Use of knowledge based scoring function (KBSF) for virtual screening and molecular docking has become an established method for drug discovery. Lack of a precise and reliable free energy function that describes several interactions including water-mediated atomic interaction between amino-acid residues and ligand makes distance based statistical measure as the only alternative. Till now all the distance based scoring functions in KBSF arena use atom singularity concept, which neglects the environmental effect of the atom under consideration. We have developed a novel knowledge-based statistical energy function for protein-ligand complexes which takes atomic environment in to account hence functional group as a singular entity. The proposed knowledge based scoring function is fast, simple to construct, easy to use and moreover it tackle the existing problem of handling molecular orientation in active site pocket. We have designed and used Functional group based Ligand retrieval (FBLR) system which can identify and detect the orientation of functional groups in ligand. This decoy searching was used to build the above KBSF to quantify the activity and affinity of high resolution protein-ligand complexes. We have proposed the probable use of these decoys in molecular build-up as a de-novo drug designing approach. We have also discussed the possible use of the said KSBF in pharmacophore fragment detection and pseudo center based fragment alignment procedure. PMID:19255647

  16. Health workforce remuneration: Comparing wage levels, ranking and dispersion of 16 occupational groups in 20 countries

    NARCIS (Netherlands)

    Tijdens, K.; de Vries, D.H.

    2011-01-01

    This article focuses on remuneration in the Human Resources for Health (HRH), comparing wage levels, ranking and dispersion of 16 HRH occupations in 20 countries (Argentina, Belarus, Belgium, Brazil, Chile, Colombia, Czech Republic, Finland, Germany, India, Mexico, Netherlands, Poland, Russian Feder

  17. Muscle Torque and its Relation to Technique, Tactics, Sports Level and Age Group in Judo Contestants

    Directory of Open Access Journals (Sweden)

    Lech Grzegorz

    2015-03-01

    Full Text Available The aim of this study was to perform a comparative analysis of maximal muscle torques at individual stages of development of athletes and to determine the relationship between muscle torques, fighting methods and the level of sports performance.

  18. Differing Levels of Superstitious Beliefs among Three Groups: Psychiatric Inpatients, Churchgoers, and Students.

    Science.gov (United States)

    Robinson, Sheryl L.

    This study investigated the level of superstitious belief among 175 persons in three categories: persons undergoing inpatient psychiatric treatment, churchgoers, and college students. A 50-item inventory consisting of positive and negative common superstitions, including a 5-item invalidity subscale, was administered. Using a 2 (male, female) x 3…

  19. Supporting Diverse Young Adolescents: Cooperative Grouping in Inclusive Middle-Level Settings

    Science.gov (United States)

    Miller, Nicole C.; McKissick, Bethany R.; Ivy, Jessica T.; Moser, Kelly

    2017-01-01

    The middle level classroom presents unique challenges to educators who strive to provide opportunities that acknowledge learner diversity in terms of social, cognitive, physical, and emotional development. This is confounded even further within inclusive middle-school classrooms where the responsibility to differentiate instruction is even more…

  20. Relationship of HbA1c variability, absolute changes in HbA1c, and all-cause mortality in type 2 diabetes

    DEFF Research Database (Denmark)

    Skriver, Mette Vinther; Sandbæk, Annelli; Kristensen, Jette Kolding;

    2015-01-01

    OBJECTIVE: We assessed the relationship of mortality with glycated hemoglobin (HbA1c) variability and with absolute change in HbA1c. DESIGN: A population-based prospective observational study with a median follow-up time of 6 years. METHODS: Based on a validated algorithm, 11 205 Danish individuals...

  1. Doping Level of Boron-Doped Diamond Electrodes Controls the Grafting Density of Functional Groups for DNA Assays.

    Science.gov (United States)

    Švorc, Ĺubomír; Jambrec, Daliborka; Vojs, Marian; Barwe, Stefan; Clausmeyer, Jan; Michniak, Pavol; Marton, Marián; Schuhmann, Wolfgang

    2015-09-02

    The impact of different doping levels of boron-doped diamond on the surface functionalization was investigated by means of electrochemical reduction of aryldiazonium salts. The grafting efficiency of 4-nitrophenyl groups increased with the boron levels (B/C ratio from 0 to 20,000 ppm). Controlled grafting of nitrophenyldiazonium was used to adjust the amount of immobilized single-stranded DNA strands at the surface and further on the hybridization yield in dependence on the boron doping level. The grafted nitro functions were electrochemically reduced to the amine moieties. Subsequent functionalization with a succinic acid introduced carboxyl groups for subsequent binding of an amino-terminated DNA probe. DNA hybridization significantly depends on the probe density which is in turn dependent on the boron doping level. The proposed approach opens new insights for the design and control of doped diamond surface functionalization for the construction of DNA hybridization assays.

  2. HbA1c is associated with severity of obstructive sleep apnea hypopnea syndrome in nondiabetic men

    Directory of Open Access Journals (Sweden)

    Nikolaos Papanas

    2009-09-01

    Full Text Available Nikolaos Papanas1, Paschalis Steiropoulos2, Evangelia Nena2, Argyris Tzouvelekis2, Efstratios Maltezos1, Georgia Trakada2, Demosthenes Bouros21Outpatient Clinic of Obesity, Diabetes and Metabolism at the 2nd Department of Internal Medicine, 2Department of Pneumonology, Democritus University of Thrace, Alexandroupolis, GreeceAbstract: The aim of this study was to examine the potential correlation of sleep characteristics with glucose metabolism in nondiabetic men with obstructive sleep apnea syndrome (OSAS. Included were 31 male patients (mean age 46.7 ± 11 years, recently diagnosed with OSAS by full polysomnography. There was a significant correlation of fasting glucose and glycosylated hemoglobin (HbA1c levels with arousal index (P = 0.047 and P = 0.014, respectively. Moreover, HbA1c levels were correlated with apnea hypopnea index (P = 0.009, a widely accepted marker of the severity of OSAS, and with percentage of sleep time with saturation of hemoglobin with oxygen as measured by pulse oximetry (SpO2 <90% (t < 90% (P = 0.010. Finally, glucose and HbA1c levels showed a significant negative correlation with average SpO2 (P = 0.013 and P = 0.012, respectively and, additionally, glucose levels with minimum SpO2 (P = 0.027 during sleep. In conclusion, severity of OSAS among nondiabetic men is associated with increased HbA1c levels and increased fasting glucose. Thus, severity of OSAS may be an additional marker of cardiovascular risk, as well as of future diabetes, in these subjects. However, further work is needed to confirm the clinical significance of these observations.Keywords: obstructive sleep apnea syndrome, glucose metabolism, glycated hemoglobin, sleep disordered breathing

  3. A Joint Forces Group: A Permanent Joint Echelon for the Operational Level of War

    Science.gov (United States)

    1998-05-21

    Government Printing Office, 1996), 539; A paper by Dr. William Gregor describes in some detail the development of the theater strategic level as well as...the current US command structure. William J. Gregor , Toward a Revolution in Civil-Military Affairs Understanding the United States Military in the...Jay Luvass, trans. G. H. Mendell and W.P. Craighill (Philadelphia: J.B. Lippincott & Co., 1862; reprint Wesport, CT: Greenwood Press, Publishers

  4. Two new beta-chain variants: Hb Tripoli [beta26(B8)Glu-->Ala] and Hb Tizi-Ouzou [beta29(B11)Gly-->Ser].

    Science.gov (United States)

    Lacan, Philippe; Becchi, Michel; Zanella-Cleon, Isabelle; Aubry, Martine; Ffrench, Martine; Couprie, Nicole; Francina, Alain

    2004-08-01

    Two new beta-globin chain variants: Hb Tripoli: codon 26, GAG-->GCG [beta26(B8)Glu-->Ala] and Hb Tizi-Ouzou: codon 29, GGC-->AGC [beta29(B11)Gly-->Ser] are described on the first exon of the beta-globin gene. The two variants are characterized by DNA sequencing and mass spectrometry (MS). Hematological abnormalities were found in the two carriers. The presence of microcytosis and hypochromia is explained by an additional homozygous 3.7 kb alpha(+) thalassemic deletion for the carrier of Hb Tizi-Ouzou. Hb Tizi-Ouzou showed a slight instability in vitro. The same hematological abnormalities associated with anemia are difficult to explain for Hb Tripoli's carrier in the absence of an alpha-globin genes abnormality and could suggest a possible abnormal splicing.

  5. SERUM PROLACTIN LEVEL IN ACNE PATIENTS VERSUS CONTROL GROUP: STUDY ON 14 TO 35 YEARS OLD WOMEN

    Directory of Open Access Journals (Sweden)

    G FAGHIHI

    2002-03-01

    Full Text Available Introduction. Androgens have a main role in acne pathogenesis. The interaction between prolactin and androgens generate the hypothesis of prolactin role in acne pathogenesis. Methods. In a case - control study, 71 women with modearte to severe acne were compaired with 71 healthy women about their serum prolactin levels. Results. Mean of serum prolactin level was 533 632 miu/lit in cases. Mean of serum prolactin level was 365 363 in control group (P > 0.05. There was a significant correlation between serum prolactin level and severity of acne. Discussion. Despite the non significant difference between serum prolactin level in acne patients and healthy women, thare was a significant relationship between serum prolactin level and severity of acne. It may be due to our small sample size. However, the more powerful studies is needed.

  6. Cortisol levels and aggression in neutered and intact free-roaming female cats living in urban social groups.

    Science.gov (United States)

    Finkler, H; Terkel, J

    2010-03-03

    Free-roaming domestic cats in urban areas often live in defined social groups, and the breeding females in these groups tend to form structures of a matrilineal nature. In recent years, resulting from the growing populations of free-roaming cats in many cities worldwide, these cats are being managed using the Trap-Neuter-Return (TNR) method. The aims of this study were to explore the hypotheses that (a) neutering reduces aggression in the females living in such social groups; and (b) if such reduction does occur, that it might be accompanied by a reduction in cortisol levels. The study was conducted on eight cat feeding groups in residential neighbourhoods in Tel Aviv, Israel. The municipal veterinary department offers TNR services upon request. Cats are collected from the feeding group and returned to their original group after neutering. We found that neutered females showed reduced aggressiveness as well as reduced cortisol levels compared to the intact females. In addition, those intact females that displayed more aggression had higher cortisol levels compared to the less aggressive intact females. Based on the results of this study it is possible to suggest for the first time a possible relationship between cortisol levels and aggression in free-roaming female domestic cats. This study is an initial step in assessing the long-term effects of Trap-Neuter-Return (TNR) on the welfare of the individual cat. If cortisol levels in female cats are reduced after neutering, partly as a result of reduced social and reproductive pressures (as expressed by lower aggression of the neutered females), it is possible that TNR has an added beneficial role in cat welfare in addition to that of control of population size.

  7. The effect of presenteeism-related health conditions on employee work engagement levels: A comparison between groups

    Directory of Open Access Journals (Sweden)

    Leon T. de Beer

    2014-02-01

    Full Text Available Orientation: Awareness of presenteeism-related health conditions is important as the prevalence of these conditions unknowingly influences performance and productivity in organisations.Research purpose: The primary objective of this study was to determine the differences in work engagement levels based on groups of presenteeism-related conditions in employees.Motivation for the study: Awareness of the impact of presenteeism-related conditions on work engagement levels can aid in the crafting of interventions to assist employees who suffer from these conditions, which in turn can boost work engagement levels.Research design, approach and method: Cross-sectional data was collected from an availability sample of employees in the manufacturing sector (N = 3387.Main findings: The results of the multi-group structural equation modelling revealed significant mean differences in work engagement levels between the groups. Practical significance tests revealed significant differences between all the groups. The largest difference was between the group who suffered from no presenteeism-related conditions and the group who suffered from all three conditions included in this study concurrently.Practical/managerial implications: Organisational stakeholders are encouraged to take note of the effects that presenteeism-related health conditions have on work engagement and to consider relevant strategies and interventions to address and alleviate symptoms in order to tend to employee health and obviate the effect on productivity.Contribution: This study found that there were clear practical differences between employees who suffer from the presenteeism-related conditions and those who suffer from none of the conditions. Furthermore, there was also a clear difference when comparing the ‘no condition’ group to a general random sample in which employees might experience some symptoms but not comorbidity.

  8. 慢性丙型肝炎合并糖尿病患者血清 HCV - RNA 载量与 FPG、FA、HbA1C 的差异性分析%A difference analysis of Fasting plasma glucose,Fructosamine and Glycated hemoglobin in serum HCV - RNA loads of chronic hepatitis C - diabetes mellitus patients

    Institute of Scientific and Technical Information of China (English)

    徐兰芝; 左维泽

    2015-01-01

    目的:了解慢性丙肝合并糖尿病(CHC - DM)患者不同血清丙型肝炎病毒核糖核酸(HCV - RNA)载量与空腹血糖(FPG)、果糖胺(FA)及糖化血红蛋白(HbA1C)的差异性。方法:选取 CHC - DM 患者120例,采用荧光定量 PCR 法检测其血清 HCV- RNA,依据病毒载量将其分为4组,病毒阴性组48例、低病毒载量组18例、中病毒载量组29例、高病毒载量组25例,采用全自动生化分析仪测定 FPG、FA、HbAlc。另外选30例正常体检者作为对照。结果:CHC - DM 组的 FPG、FA、HbA1C 的均值水平高于健康对照组,差异具有统计学意义(P <0.05),不同 HCV - RNA 载量组间三者差异有统计学意义(P <0.05),随着病毒载量的增加,FPG、FA、HbA1C 水平有增高趋势。结论:CHC - DM 患者 FPG、FA、HbA1C 水平随着病毒载量的增加有增高趋势,应密切检测其动态变化。%Objective To realize the difference of Fasting plasma glucose,Fructosamine and Glycated hemoglobin in Varying serum HCV - RNA loads of chronic hepatitis C - diabetes mellitus patients. Method One hundred and twenty cases of chronic hepatitis C - diabe-tes mellitus patients were divided into 4 groups,48 cases in virus negative group with virus load of HCV - RNA,18 cases in low virus load group with virus load of HCV - RNA,29 cases in medium virus load group with virus load of HCV - RNA,25 cases in high virus load group with virus load of HCV - RNA. Thirty normal health examination were allocated as control group. Results The average level of FPG,FA and HbA1C in CHC - DM group was significantly higher than that of healthy controls(P <0. 05),The average level of FPG,FA and HbA1C in Va-rying serum HCV - RNA loads groups was statistically significant(P <0. 05). Conclusion With the increase of virus load,the level of FPG, FA and HbA1C had the trend of increase,we should closely monitor the dynamic change of them.

  9. NITRATE CONVERSION OF HB-LINE REILLEXTM HPQ RESIN

    Energy Technology Data Exchange (ETDEWEB)

    Steimke, J.; Williams, M.; Steeper, T.; Leishear, R.

    2012-05-29

    Reillex{trademark} HPQ ion exchange resin is used by HB Line to remove plutonium from aqueous streams. Reillex{trademark} HPQ resin currently available from Vertellus Specialties LLC is a chloride ionic form, which can cause stress corrosion cracking in stainless steels. Therefore, HB Line Engineering requested that Savannah River National Laboratory (SRNL) convert resin from chloride form to nitrate form in the Engineering Development Laboratory (EDL). To perform this task, SRNL treated two batches of resin in 2012. The first batch of resin from Reilly Industries Batch 80302MA was initially treated at SRNL in 2001 to remove chloride. This batch of resin, nominally 30 liters, has been stored wet in carboys since that time until being retreated in 2012. The second batch of resin from Batch 23408 consisted of 50 kg of new resin purchased from Vertellus Specialties in 2012. Both batches were treated in a column designed to convert resin using downflow of 1.0 M sodium nitrate solution through the resin bed followed by rinsing with deionized water. Both batches were analyzed for chloride concentration, before and after treatment, using Neutron Activation Analysis (NAA). The resin specification [Werling, 2003] states the total chlorine and chloride concentration shall be less than 250 ppm. The resin condition for measuring this concentration is not specified; however, in service the resin would always be fully wet. Measurements in SRNL showed that changing from oven dry resin to fully wet resin, with liquid in the particle interstices but no supernatant, increases the total weight by a factor of at least three. Therefore, concentration of chlorine or chloride expressed as parts per million (ppm) decreases by a factor of three. Therefore, SRNL recommends measuring chlorine concentration on an oven dry basis, then dividing by three to estimate chloride concentration in the fully wet condition. Chloride concentration in the first batch (No.80302MA) was nearly the same

  10. Quality assurance in radiotherapy: the importance of medical physics staffing levels. Recommendations from an ESTRO/EFOMP joint task group.

    Science.gov (United States)

    Belletti, S; Dutreix, A; Garavaglia, G; Gfirtner, H; Haywood, J; Jessen, K A; Lamm, I L; Mijnheer, B; Noël, A; Nüsslin, F; Rosenow, U; Schneider, P; Seelentag, W; Sheriff, S; Svensson, H; Thwaites, D

    1996-10-01

    The safe application of ionising radiation for diagnosis and therapy requires a high level of knowledge of the underlying processes and of quality assurance. Sophisticated modern equipment can be used effectively for complicated diagnostic and therapeutic techniques only with adequate physics support. In the light of recent analyses and recommendations by national and international societies a joint working group of representatives from ESTRO (European Society for Therapeutic Radiology and Oncology) and from EFOMP (European Federation of Organisations for Medical Physics) was set up to assess the necessary staffing levels for physics support to radiotherapy. The method used to assess the staffing levels, the resulting recommendations and examples of their practical application are described.

  11. Group-Level Coping as a Moderator between Heterosexism and Sexism and Psychological Distress in Sexual Minority Women

    Science.gov (United States)

    Szymanski, Dawn M.; Owens, Gina P.

    2009-01-01

    The purpose of this study was: (1) to examine concurrently the relationship between heterosexist events and sexist events and psychological distress and (2) to investigate sexual orientation-based and gender-based group-level coping as potential moderators of the heterosexism-distress and sexism-distress links among 282 lesbian and bisexual women.…

  12. Application of Group-Level Item Response Models in the Evaluation of Consumer Reports about Health Plan Quality

    Science.gov (United States)

    Reise, Steven P.; Meijer, Rob R.; Ainsworth, Andrew T.; Morales, Leo S.; Hays, Ron D.

    2006-01-01

    Group-level parametric and non-parametric item response theory models were applied to the Consumer Assessment of Healthcare Providers and Systems (CAHPS[R]) 2.0 core items in a sample of 35,572 Medicaid recipients nested within 131 health plans. Results indicated that CAHPS responses are dominated by within health plan variation, and only weakly…

  13. Pulmonary levels of high-mobility group box 1 during mechanical ventilation and ventilator-associated pneumonia

    NARCIS (Netherlands)

    van Zoelen, Marieke A D; Ishizaka, Akitoshi; Wolthuls, Esther K; Choi, Goda; van der Poll, Tom; Schultz, Marcus J

    2008-01-01

    High-mobility group box (HMGB) 1 is a recently discovered proinflammatory mediator that contributes to acute lung injury. We determined HMGB-1 levels in bronchoalveolar lavage fluid of patients during mechanical ventilation (MV) and ventilator-associated pneumonia (VAP). Bronchoalveolar lavage fluid

  14. A Psychometric Evaluation of the Group Environment Questionnaire in a Sample of Elite and Regional Level Greek Volleyball Players

    Science.gov (United States)

    Ntoumanis, Nikos; Aggelonidis, Yannis

    2004-01-01

    The purpose of this study was to examine the psychometric properties of the Group Environment Questionnaire (GEQ) adapted to the Greek language. The sample consisted of 586 male and female volleyball players of elite and regional level status. Data were analysed from three time points of a competitive season. For each time point, seven competing…

  15. Enhanced-Group Moore Method: Effects on van Hiele Levels of Geometric Understanding, Proof-Construction Performance and Beliefs

    Science.gov (United States)

    Salazar, Douglas A.

    2012-01-01

    This study aimed to improve the van Hiele levels of geometric understanding, proof-construction performance and beliefs about proofs of the research respondents: future mathematics teachers exposed to the traditional (instructor-based) method and the enhanced-group Moore method. By using the quasi-experimental method of research, the study…

  16. Should Studies of Diabetes Treatment Stratification Correct for Baseline HbA1c?

    Directory of Open Access Journals (Sweden)

    Angus G Jones

    Full Text Available Baseline HbA1c is a major predictor of response to glucose lowering therapy and therefore a potential confounder in studies aiming to identify other predictors. However, baseline adjustment may introduce error if the association between baseline HbA1c and response is substantially due to measurement error and regression to the mean. We aimed to determine whether studies of predictors of response should adjust for baseline HbA1c.We assessed the relationship between baseline HbA1c and glycaemic response in 257 participants treated with GLP-1R agonists and assessed whether it reflected measurement error and regression to the mean using duplicate 'pre-baseline' HbA1c measurements not included in the response variable. In this cohort and an additional 2659 participants treated with sulfonylureas we assessed the relationship between covariates associated with baseline HbA1c and treatment response with and without baseline adjustment, and with a bias correction using pre-baseline HbA1c to adjust for the effects of error in baseline HbA1c.Baseline HbA1c was a major predictor of response (R2 = 0.19,β = -0.44,p<0.001.The association between pre-baseline and response was similar suggesting the greater response at higher baseline HbA1cs is not mainly due to measurement error and subsequent regression to the mean. In unadjusted analysis in both cohorts, factors associated with baseline HbA1c were associated with response, however these associations were weak or absent after adjustment for baseline HbA1c. Bias correction did not substantially alter associations.Adjustment for the baseline HbA1c measurement is a simple and effective way to reduce bias in studies of predictors of response to glucose lowering therapy.

  17. Detection of HbA(1c) by boronate affinity immunoassay using bacterial magnetic particles.

    Science.gov (United States)

    Tanaka, T; Matsunaga, T

    2001-12-01

    We have developed a boronate affinity immunoassay system using m-aminophenylboronic acid (mAPB) coupling to bacterial magnetic particles (BMPs). Homobifunctional crosslinker, Bis-(succcimidyl)suberate (BS3), was employed for preparation of mAPB-BMPs conjugates (mAPB-BMPs). Quantities of HbA(1c) on mAPB-BMPs were evaluated based on luminescence from alkaline phosphatase-conjugated anti-Hb antibody (ALP-antibody) binding to HbA(1c) on the BMP surface. The binding of HbA(1c) to mAPB-BMPs occurred gradually and was almost completed within 10 mm. The coupling reaction is enhanced due to static electric interaction between the positive charges on HbA(1c) and negative charges on BMPs. The amount of HbA(1c) binding to mAPB-BMPs increased with increasing sodium chloride concentrations in the range of 0-100 mM. However, the amount of Hb binding to mAPB-BMPs also increased in high concentration of sodium chloride. The Hb binding to mAPB-BMPs was detached from mAPB-BMPs when Hb-mAPB-BMPs were washed with low salt buffer. This indicates that Hb is nonspecifically adsorbed onto the surface of mAPB-BMPs in high concentration of sodium chloride. These results suggest that selective separation of HbA(1c) using mAPB-BMPs can be achieved with these conditions. A dose-response curve was obtained between luminescence intensity and HbA(1c) concentration using a fully automated boronate affinity immunoassay. A linear relationship between luminescence intensity and HbA(1c) concentration was obtained in the range of 10-10(4) ng/ml.

  18. Glycated Hemoglobin (HbA1c): Clinical Applications of a Mathematical Concept

    Science.gov (United States)

    Leow, Melvin Khee Shing

    2016-01-01

    Background and purpose: Glycated hemoglobin (HbA1c) reflects the cumulative glucose exposure of erythrocytes over a preceding time frame proportional to erythrocyte survival. HbA1c is thus an areal function of the glucose-time curve, an educationally useful concept to aid teaching and clinical judgment. Methods: An ordinary differential equation is formulated as a parsimonious model of HbA1c. The integrated form yields HbA1c as an area-under-the-curve (AUC) of a glucose-time profile. The rate constant of the HbA1c model is then derived using the validated regression equation in the ADAG study that links mean blood glucose and HbA1c with a very high degree of goodness-of-fit. Results: This model has didactic utility to enable patients, biomedical students and clinicians to appreciate how HbA1c may be conceptually inferred from discrete blood glucose values using continuous glucose monitoring system (CGMS) or self-monitored blood glucose (SMBG) glucometer readings as shown in the examples. It can be appreciated how hypoglycemia can occur with rapid HbA1c decline despite poor glycemic control. Conclusions: Being independent of laboratory assay pitfalls, computed ‘virtual’ HbA1c serves as an invaluable internal consistency cross-check against laboratory-measured HbA1c discordant with SMBG readings suggestive of inaccurate/fraudulent glucometer records or hematologic disorders including thalassemia and hemoglobinopathy. This model could be implemented within portable glucometers, CGMS devices and even smartphone apps for deriving tentative ‘virtual’ HbA1c from serial glucose readings as an adjunct to measured HbA1c. Such predicted ‘virtual’ HbA1c readily accessible via glucometers may serve as feedback to modify behavior and empower diabetic patients to achieve better glycemic control. PMID:27708483

  19. P(3HB Based Magnetic Nanocomposites: Smart Materials for Bone Tissue Engineering

    Directory of Open Access Journals (Sweden)

    Everest Akaraonye

    2016-01-01

    Full Text Available The objective of this work was to investigate the potential application of Poly(3-hydroxybutyrate/magnetic nanoparticles, P(3HB/MNP, and Poly(3-hydroxybutyrate/ferrofluid (P(3HB/FF nanocomposites as a smart material for bone tissue repair. The composite films, produced using conventional solvent casting technique, exhibited a good uniform dispersion of magnetic nanoparticles and ferrofluid and their aggregates within the P(3HB matrix. The result of the static test performed on the samples showed that there was a 277% and 327% increase in Young’s modulus of the composite due to the incorporation of MNP and ferrofluid, respectively. The storage modulus of the P(3HBMNP and P(3HB/FF was found to have increased to 186% and 103%, respectively, when compared to neat P(3HB. The introduction of MNP and ferrofluid positively increased the crystallinity of the composite scaffolds which has been suggested to be useful in bone regeneration. The total amount of protein absorbed by the P(3HB/MNP and P(3HB/FF composite scaffolds also increased by 91% and 83%, respectively, with respect to neat P(3HB. Cell attachment and proliferation were found to be optimal on the P(HB/MNP and P(3HB/FF composites compared to the tissue culture plate (TCP and neat P(3HB, indicating a highly compatible surface for the adhesion and proliferation of the MG-63 cells. Overall, this work confirmed the potential of using P(3HB/MNP and P(3HB/FF composite scaffolds in bone tissue engineering.

  20. Studying the effect of Pingpang exercise on HbA1 c of type 2 diabetes%乒乓球运动对2型糖尿病患者糖化血红蛋白影响的研究

    Institute of Scientific and Technical Information of China (English)

    谷颖

    2011-01-01

    Observe the effect of Pingpang on HbA1c for 50 persons with type 2 diabetic object. They are divided into exercise group and control group at random. Exercise group go on 4 month doing Pingpang exercise. Measure separately the HbA1c, blood sugar and blood fat of the control and exercise group before exercises and next day after 4 months under empty stomach state. Pingpang exercise could improve the index of HbA1c, blood sugar and blood fat of type 2 diabetic (P<0. 05 and P<0. 01 separately), and it has significant differences compare with the control group. Pingpang exercise is helpful to reduce HbA1c level and favorable to the diabetic's recovery.%随机选取50名2型糖尿病患者,分为运动组和对照组,观察乒乓球运动对其糖化血红蛋白的影响.运动组进行4个月的乒乓球运动.检测实验前后安静空腹状态下糖化血红蛋白以及常规血糖和血脂指标.乒乓球锻炼有助于2型糖尿病患者糖化血红蛋白、血糖和血脂指标的改善(分别P<0.05和P<0.01).乒乓球运动锻炼对2型糖尿病患者有较好的辅助治疗作用,是有效的康复手段.

  1. Phylogeny of a genomically diverse group of elymus (poaceae allopolyploids reveals multiple levels of reticulation.

    Directory of Open Access Journals (Sweden)

    Roberta J Mason-Gamer

    Full Text Available The grass tribe Triticeae (=Hordeeae comprises only about 300 species, but it is well known for the economically important crop plants wheat, barley, and rye. The group is also recognized as a fascinating example of evolutionary complexity, with a history shaped by numerous events of auto- and allopolyploidy and apparent introgression involving diploids and polyploids. The genus Elymus comprises a heterogeneous collection of allopolyploid genome combinations, all of which include at least one set of homoeologs, designated St, derived from Pseudoroegneria. The current analysis includes a geographically and genomically diverse collection of 21 tetraploid Elymus species, and a single hexaploid species. Diploid and polyploid relationships were estimated using four molecular data sets, including one that combines two regions of the chloroplast genome, and three from unlinked nuclear genes: phosphoenolpyruvate carboxylase, β-amylase, and granule-bound starch synthase I. Four gene trees were generated using maximum likelihood, and the phylogenetic placement of the polyploid sequences reveals extensive reticulation beyond allopolyploidy alone. The trees were interpreted with reference to numerous phenomena known to complicate allopolyploid phylogenies, and introgression was identified as a major factor in their history. The work illustrates the interpretation of complicated phylogenetic results through the sequential consideration of numerous possible explanations, and the results highlight the value of careful inspection of multiple independent molecular phylogenetic estimates, with particular focus on the differences among them.

  2. Phylogeny of a genomically diverse group of elymus (poaceae) allopolyploids reveals multiple levels of reticulation.

    Science.gov (United States)

    Mason-Gamer, Roberta J

    2013-01-01

    The grass tribe Triticeae (=Hordeeae) comprises only about 300 species, but it is well known for the economically important crop plants wheat, barley, and rye. The group is also recognized as a fascinating example of evolutionary complexity, with a history shaped by numerous events of auto- and allopolyploidy and apparent introgression involving diploids and polyploids. The genus Elymus comprises a heterogeneous collection of allopolyploid genome combinations, all of which include at least one set of homoeologs, designated St, derived from Pseudoroegneria. The current analysis includes a geographically and genomically diverse collection of 21 tetraploid Elymus species, and a single hexaploid species. Diploid and polyploid relationships were estimated using four molecular data sets, including one that combines two regions of the chloroplast genome, and three from unlinked nuclear genes: phosphoenolpyruvate carboxylase, β-amylase, and granule-bound starch synthase I. Four gene trees were generated using maximum likelihood, and the phylogenetic placement of the polyploid sequences reveals extensive reticulation beyond allopolyploidy alone. The trees were interpreted with reference to numerous phenomena known to complicate allopolyploid phylogenies, and introgression was identified as a major factor in their history. The work illustrates the interpretation of complicated phylogenetic results through the sequential consideration of numerous possible explanations, and the results highlight the value of careful inspection of multiple independent molecular phylogenetic estimates, with particular focus on the differences among them.

  3. Effect of cooperation level of group on punishment for non-cooperators: a functional magnetic resonance imaging study.

    Directory of Open Access Journals (Sweden)

    Fumitoshi Kodaka

    Full Text Available Sometimes we punish non-cooperators in our society. Such behavior could be derived from aversive emotion for inequity (inequity aversion to make non-cooperators cooperative. Thus, punishing behavior derived from inequity is believed to be important for maintaining our society. Meanwhile, our daily experiences suggest that the degree of cooperation by the members of society (cooperation level of the group could change the punishing behavior for non-cooperators even if the inequity were equal. Such effect of the cooperation level of the group cannot be explained by simple inequity aversion. Although punishment-related brain regions have been reported in previous functional magnetic resonance imaging (fMRI study, little is known about such regions affected by the cooperation level of the group. In the present fMRI study, we investigated the effect of the cooperation level of the group on the punishing behavior for non-cooperators and its related brain activations by a paradigm in which the degree of the cooperative state varied from low to high. Punishment-related activations were observed in brain regions such as the anterior insula, dorsolateral prefrontal cortex (DLPFC, and anterior cingulate cortex (ACC. The quantity of punishment in a high cooperation context was greater than in a low cooperation context, and activation in the right DLPFC and ACC in a high cooperation context showed greater activity than in a low cooperation context. This indicates that the cooperation level of the group, as well as aversive emotion for inequity, is the important factor of punishing behavior.

  4. Group-level spatio-temporal pattern recovery in MEG decoding using multi-task joint feature learning.

    Science.gov (United States)

    Kia, Seyed Mostafa; Pedregosa, Fabian; Blumenthal, Anna; Passerini, Andrea

    2017-06-15

    The use of machine learning models to discriminate between patterns of neural activity has become in recent years a standard analysis approach in neuroimaging studies. Whenever these models are linear, the estimated parameters can be visualized in the form of brain maps which can aid in understanding how brain activity in space and time underlies a cognitive function. However, the recovered brain maps often suffer from lack of interpretability, especially in group analysis of multi-subject data. To facilitate the application of brain decoding in group-level analysis, we present an application of multi-task joint feature learning for group-level multivariate pattern recovery in single-trial magnetoencephalography (MEG) decoding. The proposed method allows for recovering sparse yet consistent patterns across different subjects, and therefore enhances the interpretability of the decoding model. Our experimental results demonstrate that the mutli-task joint feature learning framework is capable of recovering more meaningful patterns of varying spatio-temporally distributed brain activity across individuals while still maintaining excellent generalization performance. We compare the performance of the multi-task joint feature learning in terms of generalization, reproducibility, and quality of pattern recovery against traditional single-subject and pooling approaches on both simulated and real MEG datasets. These results can facilitate the usage of brain decoding for the characterization of fine-level distinctive patterns in group-level inference. Considering the importance of group-level analysis, the proposed approach can provide a methodological shift towards more interpretable brain decoding models. Copyright © 2017 Elsevier B.V. All rights reserved.

  5. Oestradiol protects against the harmful effects of fluoride more by increasing thiol group levels than scavenging hydroxyl radicals.

    Science.gov (United States)

    Dlugosz, Anna; Roszkowska, Anna; Zimmer, Mariusz

    2009-12-01

    The aim of the study was to investigate the role of oestrogens in free radical detoxication upon exposure to fluoride. Interactions between xenobiotics and oestrogens need to be investigated, especially as many chemicals interact with the oestrogen receptor. It is still unknown whether free radical-generating xenobiotics can influence the antioxidative ability of oestradiol (E(2)). In an in vitro examination of human placental mitochondria, thiobarbituric active reagent species (TBARS), hydroxyl radical ((*)OH) generation and protein thiol (-SH) groups were detected. 17beta-E(2) was examined in physiological (0.15-0.73 nM) and experimental (1-10 microM) concentrations and sodium fluoride (NaF) in concentrations of 6-24 microM. E(2) in all the concentrations significantly decreased lipid peroxidation measured as the TBARS level, in contrast to NaF, which increased lipid peroxidation. Lipid peroxidation induced by NaF was decreased by E(2). The influence of E(2) on (*)OH generation was not very significant and depended on the E(2 )concentration. The main mechanism of E(2) protection in NaF exposure appeared to be connected with the influence of E(2 )on thiol group levels, not (*)OH scavenging ability. The E(2) in concentrations 0.44-0.73 nM and 1-10 microM significantly increased the levels of -SH groups, in contrast to NaF, which significantly decreased them. E(2) at every concentration reversed the harmful effects of NaF on -SH group levels. No unfavourable interactions in the influence of E(2) and NaF on TBARS production, (*)OH generation, or -SH group levels were observed. The results suggest that postmenopausal women could be more sensitive to NaF-initiated oxidative stress.

  6. Approaching confidentiality at a familial level in genomic medicine: a focus group study with healthcare professionals

    Science.gov (United States)

    Dheensa, Sandi; Fenwick, Angela; Lucassen, Anneke

    2017-01-01

    Objectives Clinical genetics guidelines from 2011 conceptualise genetic information as confidential to families, not individuals. The normative consequence of this is that the family's interest is the primary consideration and genetic information is shared unless there are good reasons not to do so. We investigated healthcare professionals' (HCPs') views about, and reasoning around, individual and familial approaches to confidentiality and how such views influenced their practice. Method 16 focus groups with 80 HCPs working in/with clinical genetics services were analysed, drawing on grounded theory. Results Participants raised seven problems with, and arguments against, going beyond the individual approach to confidentiality. These problems fell into two overlapping categories: ‘relationships’ and ‘structures’. Most participants had never considered ways to—or thought it was impossible to—treat familial genetic information and personal information differently. They worried that putting the familial approach into practice could disrupt family dynamics and erode patient trust in the health service. They also thought they had insufficient resources to share information and feared that sharing might change the standard of care and make them more vulnerable to liability. Conclusions A familial approach to confidentiality has not been accepted or adopted as a standard, but wider research suggests that some of the problems HCPs perceived are surmountable and sharing in the interest of the family can be achieved. However, further research is needed to explore how personal and familial genetic information can be separated in practice. Our findings are relevant to HCPs across health services who are starting to use genome tests as part of their routine investigations. PMID:28159847

  7. Microsporogenesis in Paspalum conspersum Schrad. (Virgata group) with different ploidy levels.

    Science.gov (United States)

    Janke, L; Souza, F H D; Valls, J F M; Pagliarini, M S

    2013-10-24

    Knowledge about the cytology and reproductive behavior of a species is indispensable for hybridization programs. This is especially true for species belonging to the genus Paspalum, among which apomixis and a wide range of ploidy levels are frequently found. Paspalum conspersum Schrad. is a robust and warm-season perennial bunchgrass native to South America. Previous studies have indicated that both tetraploid and hexaploid races exist in this species; however, only information related to tetraploids has been applied to another taxon. In this study, a cytological investigation in two Brazilian accessions collected in different regions revealed tetraploidy in the accession BRA-012823 (2n = 4x = 40), with chromosome pairing in bivalents and normal meiosis and tetrad formation, and pentaploidy (2n = 5x = 50) in the accession BRA-022748, which presented total asynapsis. In this latter accession, 50 univalents could be scored at diakinesis. After alignment at the metaphase plate, sister chromatids segregated to the poles. Only one meiotic division (equational) occurred, and after cytokinesis, 100% of the dyads that formed had 2n microspores. The meiotic behavior during microsporogenesis, which showed 10 delayed univalents to reach the metaphase plate, suggests that this accession is a recent natural hybrid constituted by a parental genome with 40 chromosomes and another with 10 chromosomes. The potential usage of these accessions in Paspalum breeding has been discussed.

  8. HB-H-6树脂血浆灌流治疗重度黄疸患者预后的影响因素分析%Analysis of prognostic factors of severe jaundice patients using HB-H-6 resin plasma perfusion

    Institute of Scientific and Technical Information of China (English)

    向慧玲; 韩涛; 杜智; 李涛; 李隽; 吕洪敏; 李庭红; 周佳美; 唐飞

    2012-01-01

    to outcomes.The effecting factors of prognosis,including age,sex,hospital stay days,number of perfusion therapy received,Child-Pugh scores before perfusion,total bilirubin (TBil) levels before perfusion,and mean TBil rebound rate were studied by univariate and multivariate logistic regression analysis.Results All 78 patients received (3.31 ±1.36) times of HB-H-6 resin plasma perfusion treatment.Child-Pugh score before perfusion,TBil (μmol/L) before perfusion and mean TBil rebound rate in improved group were significantly lower than those in ineffective group [Child-Pugh score before perfusion:8.06 ± 1.01 vs.9.44 ± 1.19; TBil before perfusion:384.29 ± 170.41 vs.504.93 ±206.88 ; mean TBil rebound rate:- (7.35 ± 20.76 )% vs. ( 37.32 ± 23.22 )% ].They were also significantly different in gender between two groups ( improved group:30 males,21 females; ineffective group:24 males,3 females,P<0.05 or P<0.01 ).Gender and mean TBil bounce rate were defined as independent significant factors influencing the clinical results by multivariate logistic regression analysis.Regression coefficient β were 5.35 and -2.82 for gender and mean TBil bounce rate respectively (x2=64.42,P=0.000).Receive operating characteristic curve (ROC curve) analysis showed that the area under the curve ( AUC ) was 0.90 (0.82,0.97 ),and mean TBil bounce rate higher than 29.5%indicated poor prognosis.No obvious side effects were observed after plasma perfusion.Conclusions Gender and mean TBil bounce rate were independent risk factors in treatment of severe jaundice with HB-H-6 resin plasma perfusion.Mean TBil bounce rate higher than 29.5% indicated a poor prognosis.

  9. An investigation of long-term effects of group music therapy on agitation levels of people with Alzheimer's Disease.

    Science.gov (United States)

    Ledger, Alison J; Baker, Felicity A

    2007-05-01

    This study aimed to investigate the long-term effects of group music therapy on agitation manifested by nursing home residents with Alzheimer's disease. A non-randomised experimental design was employed with one group receiving weekly music therapy (n = 26) and another group receiving standard nursing home care (n = 19). Agitation levels were measured five times over one year using the Cohen-Mansfield Agitation Inventory (Cohen-Mansfield, J. (1989). Agitation in the elderly. In N. Billig & P. V. Rabins (Eds.), Issues in geriatric psychiatry (pp. 101-113). Basel, Switzerland: Karger). Although music therapy participants showed short-term reductions in agitation, there were no significant differences between the groups in the range, frequency, and severity of agitated behaviours manifested over time. Multiple measures of treatment efficacy are necessary to better understand the long-term effects music therapy programs have on this population.

  10. Clinical impact of the ERBP Working Group 2010 Recommendations for the anemia management in chronic kidney disease not on dialysis: ACERCA study.

    Science.gov (United States)

    Martínez-Castelao, Alberto; Cases, Aleix; Carballada, Alberto Torre; Iranzo, Javier Torralba; Bronsoms, Josep; Vallès-Prats, Martí; Monserrat, Daniel Torán; Jimenez, Elisabet Masso

    2015-01-01

    The Anemia Working Group of ERBP in 2010 recommended a target hemoglobin (Hb) level in the range of 11-12 g/dL, without intentionally exceeding 13 g/dL during the treatment with erythropoiesis stimulating agents (ESAs). This study evaluated if there was a clinical impact of this statement in the anemia management of chronic kidney disease (CKD) patients treated with ESAs not on dialysis in routine clinical practice in Spain. This was an observational and cross-sectional study carried out in CKD patients not on dialysis in Spain who initiated ESA treatment (naïve), or were shifted from a previous ESA to another ESAs (converted) since January 2011. Of 441 patients evaluated, 67.6% were naïve and 32.4% were converted. At the study visit, 42.5% of naïve patients achieved the Hb target of 11-12 g/dL, with a mean Hb of 11.3±1.3 g/dL (vs 10.1±0.9 g/dL at the start of ESA therapy). Only 35.3% of converted patients maintained Hb levels within the recommended target at the study visit. Yet, 8.2% of naïve patients and 7.9% of those converted had Hb levels >13 g/dL. Hb levels were similar across subgroups of patients, regardless of the presence of significant comorbidities. Anemia management in CKD patients treated with ESAs by Spanish nephrologists seems to be aimed at preventing Hb levels renal anemia management in the clinical setting. Copyright © 2015. Published by Elsevier España, S.L.U.

  11. Markers of glycemic control in the mouse: comparisons of 6-h- and overnight-fasted blood glucoses to Hb A1c

    OpenAIRE

    Han, Byoung Geun; Hao, Chuan-Ming; Elena E. Tchekneva; Wang, Ying-Ying; Lee, Chieh Allen; Ebrahim, Benyamin; Raymond C Harris; Kern, Timothy S.; Wasserman, David H.; Breyer, Matthew D; Qi, Zhonghua

    2008-01-01

    The present studies examined the relationship between fasting blood glucose and Hb A1c in C57BL/6J, DBA/2J, and KK/HlJ mice with and without diabetes mellitus. Daily averaged blood glucose levels based on continuous glucose monitoring and effects of 6-h vs. overnight fasting on blood glucose were determined. Daily averaged blood glucose levels were highly correlated with Hb A1c, as determined with a hand-held automated device using an immunodetection method. R2 values were 0.90, 0.95, and 0.9...

  12. Comparative clinical evaluation of glycosylated haemoglobin level in healthy and chronic periodontitis patients: A chairside diagnostic method

    Directory of Open Access Journals (Sweden)

    T S Srinivasa

    2015-01-01

    Full Text Available Objective and Background: Glycosylated haemoglobin (HbA1c level can consequently be interpreted as an average of the blood glucose present over the past 3-4 months. Periodontitis is associated with glycemic control in patients with diabetes. The purpose of this study was to determine the level of HbA1c in healthy and periodontitis patients who were previously not diagnosed with diabetes mellitus. Materials and Methods: A total of 40 patients were selected for study and divided into two groups. Group 1 included patients with a healthy periodontium, and Group 2 included patients suffering from chronic periodontitis. Finger stick blood was collected by special collection unit (A1CNOW +® Bayer Health Care, Tarrytown New York, USA, for estimating level of HbA1c. Result: Both groups showed similar HbA1c levels clinically with slight increase in levels in the test group, but was statistically significant (test - 5.66 ± 0.35%, control - 5.17 ± 0.3% P = 0.003. Conclusion: Indians are at a high-risk of developing periodontitis and diabetes. These data suggest a possible link between periodontitis and glycemic control in nondiabetic individuals, periodontal disease may be a potential contributor to the development of type 2 diabetes.

  13. Modelling galaxy spectra at redshifts 0.2Hb and [OIII]/Hb line ratios

    CERN Document Server

    Contini, M

    2014-01-01

    We present the detailed modelling of line spectra emitted from galaxies at redshifts 0.2Hb line ratios. The results show that [OII]3727+3729/Hb and [OIII]5007+4959/Hb are not sufficient to constrain the models. The data at least of an auroral line, e.g. [OIII]4363, should be known. We have found by modelling the spectra observed from ultrastrong emission line galaxy and faint galaxy samples, O/H relative abundances ranging between 1.8 X 10^{-4} and 6.6 X 10^{-4}.

  14. Hb Bronte or alpha93(FG5)Val-->Gly: a new unstable variant of the alpha2-globin gene, associated with a mild alpha(+)-thalassemia phenotype.

    Science.gov (United States)

    Lacerra, Giuseppina; Testa, Rosario; De Angioletti, Maria; Schilirò, Gino; Carestia, Clementina

    2003-08-01

    We report a new unstable variant identified in three carriers of a family from East Sicily; it was named Hb Bronte after the place from which the family originated. DNA sequencing from nucleotides -181 to +894 (alpha1) and to +884 (alpha2) revealed a GTG-->GGG substitution at codon 93 of the alpha2-globin gene. The MCV and MCH values were at the lower end of the normal range in the carriers. On cation exchange high performance liquid chromatography (HPLC), the Hb A2 level was apparently increased to around 6%, and a small abnormal peak (0.3-0.4%) was detected after Hb A2. Two abnormal bands were detected by cellulose acetate electrophoresis: a major band (about 3-4%) migrated between Hb A and Hb F; a minor band (<1%) migrated between Hb A2 and carbonic anhydrase. Normal values of Hb A2 were detected by DEAE microchromatography. On reversed phase HPLC the variant chain was not detected, and most likely it was eluted with the alpha chain peak. The isopropanol stability test was very slightly positive in the carriers. Hemolytic symptoms were absent with the exception of indirect bilirubin, which was at high borderline in 2/3 carriers. In biosynthesis in vitro, the specific activity of the alpha chains was much higher than that of the beta-globin chains, and the alpha/beta biosynthetic ratio in the mother and proband was of the beta-thalassemia (thal) type (2.24 and 2.54, respectively). Time course experiments showed that the increase of the 3H-specific activity of the peak containing normal and variant alpha chains was not linear and was much higher than that of beta chains; moreover, the alpha/beta biosynthetic ratio varied during the 2 hours incubation.

  15. Variations in erythrocyte antioxidant levels and lipid peroxidation status and in serum lipid profile parameters in relation to blood haemoglobin A1c values in individuals with type 2 diabetes mellitus.

    Science.gov (United States)

    Annadurai, Thangaraj; Vasanthakumar, Azhagarsamy; Geraldine, Pitchairaj; Thomas, Philip A

    2014-07-01

    The present study aimed to evaluate the antioxidant and lipid peroxidation status in erythrocytes and serum lipid profile parameters, in relation to haemoglobin A1c (HbA1c) concentrations, in patients with type 2 diabetes mellitus and in normal healthy individuals. Sixty test individuals with diabetes and 15 control individuals were categorized as: Group I, control (non-diabetes); Group II, individuals with diabetes with HbA1c levels ≤7.0% (53 mmol/mol); Group III, individuals with diabetes with HbA1c levels between 7.1 and 8.0% (54 and 64 mmol/mol); Group IV, individuals with diabetes with HbA1c levels between 8.1 and 9.0% (65 and 75 mmol/mol); Group V, individuals with diabetes with HbA1c levels >9.0% (75 mmol/mol). Blood samples were collected to measure: blood glucose and HbA1c levels; haemolysate levels of enzymatic antioxidants and non-enzymatic antioxidants and malondialdehyde (MDA); and serum total cholesterol, triglyceride and high-density lipoprotein (HDL)-cholesterol levels. Correlations between blood HbA1c values and all parameters were sought. Significantly lower mean activities/levels of antioxidant parameters and significantly higher mean levels of MDA were noted in haemolysate samples from patients with diabetes than in those from control individuals. Significantly higher mean serum concentrations of total cholesterol and triglycerides and significantly lower mean concentrations of HDL-cholesterol were noted in patients with diabetes than in control individuals. Further, moderate to strong correlations were observed between values of antioxidants, MDA and lipid profile parameters and blood concentrations of HbA1c. These results suggest that HbA1c values may be potentially useful not only to indicate long-term glycemic control to indicate onset of complications at a clinically detectable level and molecular level. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  16. Geometry of mixed states and degeneracy structure of geometric phases for multi-level quantum systems. A unitary group approach

    CERN Document Server

    Ercolessi, E; Morandi, G; Mukunda, N

    2001-01-01

    We analyze the geometric aspects of unitary evolution of general states for a multilevel quantum system by exploiting the structure of coadjoint orbits in the unitary group Lie algebra. Using the same method in the case of SU(3) we study the effect of degeneracies on geometric phases for three-level systems. This is shown to lead to a highly nontrivial generalization of the result for two-level systems in which degeneracy results in a "monopole" structure in parameter space. The rich structures that arise are related to the geometry of adjoint orbits in SU(3). The limiting case of a two-level degeneracy in a three-level system is shown to lead to the known monopole structure.

  17. Sickle cell anemia and α-thalassemia: a modulating factor in homozygous HbS/S patients in Oman.

    Science.gov (United States)

    Hassan, S M; Al Muslahi, M; Al Riyami, M; Bakker, E; Harteveld, C L; Giordano, P C

    2014-01-01

    We report the general phenotype severity and the hematological presentation in a cohort of 125 sickle cell anemia (SCA) patients with identical homozygous HbS/S genotype and categorized by identical β(S) haplotype, both with and without alpha thalassemia. No clear general phenotype correlation was found when patients were compared regardless of the haplotype but overall, patients with homozygous alpha thalassemia (α-/α-) had the highest Hb, HCT, RBC and the lowest MCV, MCH and MCHC levels. When patients with identical haplotype were compared, the mildest hematological and clinical conditions were observed in patients of the Asian/Asian haplotype, also known as Arab-Indian haplotype, and carriers of α-thalassemia, suggesting an additional ameliorating effect of alpha thalassemia. In conclusion, our results show that alpha thalassemia improves the hematological conditions but amelioration of the general disease severity is only noticed when compared in cohorts of the same haplotype.

  18. Hb Tsukumi [beta117(G19)His-->Tyr]: a new hemoglobin variant found in a Japanese male.

    Science.gov (United States)

    Oribe, Y; Hamaguchi, K; Kusuda, Y; Harano, K; Harano, T; Iwasaki, Y; Kotegawa, K; Sakata, T

    2000-05-01

    We accidentally observed an abnormal elution pattern on high performance liquid chromatogram when we examined the Hb A1c level in a 65-year-old male patient who suffered from pneumoconiosis and alcoholic liver injury. The value of the glycated fraction was within the normal range but the elution patterns on high performance liquid chromatography varied with the glycohemoglobin analyzers. Isoelectrofocusing and urea-cellulose column chromatography showed an anomalous fast-moving beta chain estimated at approximately 47%. The instability test of the hemolysate was slightly positive. Structural analysis demonstrated that the mutant was consisted by a substitution of His-Tyr at beta117. This new variant was named Hb Tsukumi for the place of residence of the patient. Additionally, the nucleotide sequence showed a change of C-->T [CAC (His)-->TAC (Tyr)] at the first base in the 117th codon of the beta gene.

  19. Two new G gamma chain variants: Hb F-Saint-Etienne [G gamma 79(EF3)Asp-->His] and Hb F-Lyon [G gamma 97(FG4)His-->Arg].

    Science.gov (United States)

    Joly, Philippe; Lacan, Philippe; Garcia, Caroline; Berger, Claire; Perier, Christian; Barro, Claire; Francina, Alain

    2008-01-01

    Two new fetal hemoglobin (Hb F) variants affecting the (G)gamma chain are reported: Hb F-Saint-Etienne [G gamma 79(EF3)Asp-->His] and Hb F-Lyon [G gamma 97(FG4)His-->Arg]. These new Hb variants were found during a neonatal screening for hemoglobinopathies but characterized a few months later by our reference laboratory. The corresponding mutations are located on the external part of the Hb molecule and seem to be clinically silent.

  20. Simultaneous dual syringe electrospinning system using benign solvent to fabricate nanofibrous P(3HB-co-4HB)/collagen peptides construct as potential leave-on wound dressing

    Energy Technology Data Exchange (ETDEWEB)

    Vigneswari, S. [Malaysian Institute of Pharmaceuticals and Nutraceuticals, NIBM, MOSTI, 11700 Penang (Malaysia); Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030 Kuala Terengganu (Malaysia); Murugaiyah, V. [School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11700 Penang (Malaysia); Kaur, G. [Institute of Research in Molecular Medicine, Universiti Sains Malaysia, 11700 Penang (Malaysia); Abdul Khalil, H.P.S. [School of Industrial Technology, Universiti Sains Malaysia, 11700 Penang (Malaysia); Amirul, A.A., E-mail: amirul@usm.my [Malaysian Institute of Pharmaceuticals and Nutraceuticals, NIBM, MOSTI, 11700 Penang (Malaysia); School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang (Malaysia); Centre of Chemical Biology, Universiti Sains Malaysia, 11900 Penang (Malaysia)

    2016-09-01

    The main focus of this study is the incorporation of collagen peptides to fabricate P(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] nano-fiber construct to further enhance surface wettability and support cell growth while harbouring desired properties for biodegradable wound dressing. Simultaneous electrospinning of nanofiber P(3HB-co-4HB)/collagen peptides construct was carried out using dual syringe system. The wettability of the constructs increased with the increase in 4HB molar fraction from 20 mol% 4HB [53.2°], P(3HB-co-35 mol%4HB)[48.9°], P(3HB-co-50 mol%4HB)[44.5°] and P(3HB-co-82 mol%4HB) [37.7°]. In vitro study carried out using mouse fibroblast cells (L929) grown on nanofiber P(3HB-co-4HB)/collagen peptides construct showed an increase in cell proliferation. In vivo study using animal model (Sprague Dawley rats) showed that nanofibrous P(3HB-co-4HB)/collagen peptides construct had a significant effect on wound contractions with the highest percentage of wound closure of 79%. Hence, P(3HB-co-4HB)/collagen peptides construct suitable for wound dressing have been developed using nano-fabrication technique. - Highlights: • Nano-fiber construct to enhance surface wettability and cell growth, harbouring desired properties as biodegradable wound dressing. • Simultaneous electrospinning of nanofiber P(3HB-co-4HB)/collagen construct using dual syringe system. • Nanofibrous construct accelerated wound healing with efficient cellular organization.

  1. Nigerian propolis improves blood glucose, glycated hemoglobin A1c, very low-density lipoprotein, and high-density lipoprotein levels in rat models of diabetes

    Science.gov (United States)

    Oladayo, Mustafa Ibrahim

    2016-01-01

    Objective: According to our previous studies, propolis of Nigerian origin showed some evidence of hypoglycemic and hypolipidemic activities in addition to its ability to ameliorate oxidative-stress-induced organ dysfunction. This study was carried out to determine whether an ethanolic extract of Nigerian propolis (EENP) improves glycated hemoglobin A1c (HbA1c), fasting plasma glucose, very low-density lipoprotein (VLDL), and high-density lipoprotein (HDL) concentrations in rats that have alloxan diabetes. Materials and Methods: Diabetes was induced with alloxan (110 mg/kg). Animals were divided into 5 groups (n = 5); Group 1 was non-diabetic receiving normal saline and Group 2 was diabetic but also received only normal saline. Groups 3, 4, and 5 were diabetic receiving 200 mg/kg propolis, 300 mg/kg propolis, and 150 mg/kg metformin, respectively, for 42 days. Results: Hyperglycemia, elevated serum level of VLDL, elevated plasma level of HbA1c, and decreased levels of HDL were observed in the diabetic untreated animals. Nigerian propolis decreased blood glucose level and serum level of VLDL but elevated HDL level. These changes were significant (P < 0.05). The levels of plasma HbA1c were also reduced in the propolis-treated groups, and the reduction was significant (P < 0.05). Conclusion: Nigerian propolis contains compounds exhibiting hypoglycemic, antihyperlipidemic, and HbA1c reducing activities. PMID:27366348

  2. Effects of Endurance Training on Lipid Metabolism and Glycosylated Hemoglobin Levels in Streptozotocin-induced Type 2 Diabetic Rats on a High-fat Diet.

    Science.gov (United States)

    Heo, Myoung; Kim, Eunjung

    2013-08-01

    [Purpose] Exercise has been recognized as a simple and economical therapeutic modality that effectively benefits patients with diabetes, for instance, increasing insulin sensitivity in type 2 diabetes. However, thus far, no studies have examined the effect of endurance training exercises on type 2 diabetes. Therefore, this study examined the effect of endurance training exercise regimens on body weight, glucose and insulin levels, lipid profiles, and HbA1c levels in STZ-induced type 2 diabetic rats on a high-fat diet. HbA1c was considered an indicator of glucose control during endurance training. [Methods] A total of 36 rats were included in this study. Diabetes was induced by administering STZ to 2 groups of 12 rats each, and, the remaining 12 rats were classified as the normal group. Biochemical parameters were measured 28 days later, and included: serum total cholesterol, triglyceride, high-density lipoprotein, glycosylated hemoglobin, glucose, and insulin levels. [Results] A significant decrease in serum TC and TG levels, and an increase in HDL cholesterol level were observed in the endurance training group. Moreover, blood glucose and HbA1c levels after 28 days of exercising were significantly lower in the endurance training group than in the control group (pdiet. We suggest that endurance training exercises may exhibit therapeutic, preventative, and protective effects against diabetes mellitus through improving lipid metabolism, glycemic control, and HbA1c levels.

  3. Effect of group size and maize silage dietary levels on behaviour, health, carcass and meat quality of Mediterranean buffaloes.

    Science.gov (United States)

    Masucci, F; De Rosa, G; Barone, C M A; Napolitano, F; Grasso, F; Uzun, P; Di Francia, A

    2016-03-01

    The effects of different dietary levels of maize silage (10% v. 36% DM) and group size (7 v. 14 animals) were assessed on growth performance and in vivo digestibility of 28 male fattening buffaloes. In addition, the effects of diet on meat quality and group size on behaviour and immune response were separately evaluated. Animals were weighed and assigned to three groups. The high silage - low size group (HL) was fed a total mixed ration (TMR) containing 36% DM of maize silage and consisted of seven animals (age 12.7±2.6 months; BW 382.2±67.7 kg at the start of the study). The low silage - low size group (LL) was fed a TMR containing 10% DM of maize silage and consisted of seven animals (age 13.0±2.7 months; BW 389.4±72.3 kg). The high silage - high size group (HH) was fed the 36% maize silage DM diet and consisted of 14 animals (age 13.9±3.25 months; BW 416.5±73.9 kg). Total space allowance (3.2 indoor+3.2 outdoor m2/animal) was kept constant in the three groups, as well as the ratio of animals to drinkers (seven animals per water bowl) and the manger space (70 cm per animal). Growth performance, carcass characteristics and digestibility were influenced neither by dietary treatment nor by group size, even if the group fed 36% maize silage diet showed a higher fibre digestibility. No effect of diet was found on meat quality. Group size did not affect the behavioural activities with the exception of drinking (1.04±0.35% v. 2.60±0.35%; P<0.01 for groups HL and HH, respectively) and vigilance (2.58±0.46% v. 1.20±0.46%; P<0.05 for groups HL and HH, respectively). Immune responses were not affected by group size.

  4. HbA1c, fasting plasma glucose and the prediction of diabetes

    DEFF Research Database (Denmark)

    Soulimane, Soraya; Simon, Dominique; Shaw, Jonathan

    2012-01-01

    With diabetes defined by HbA1c≥6.5% and/or FPG≥7.0mmol/l and/or diabetes treatment, we investigated HbA1c and fasting plasma glucose (FPG) thresholds/change-points above which the incidence of diabetes increases.......With diabetes defined by HbA1c≥6.5% and/or FPG≥7.0mmol/l and/or diabetes treatment, we investigated HbA1c and fasting plasma glucose (FPG) thresholds/change-points above which the incidence of diabetes increases....

  5. HbA1c, fasting plasma glucose and the prediction of diabetes

    DEFF Research Database (Denmark)

    Soulimane, Soraya; Simon, Dominique; Shaw, Jonathan

    2012-01-01

    With diabetes defined by HbA1c≥6.5% and/or FPG≥7.0mmol/l and/or diabetes treatment, we investigated HbA1c and fasting plasma glucose (FPG) thresholds/change-points above which the incidence of diabetes increases.......With diabetes defined by HbA1c≥6.5% and/or FPG≥7.0mmol/l and/or diabetes treatment, we investigated HbA1c and fasting plasma glucose (FPG) thresholds/change-points above which the incidence of diabetes increases....

  6. First observation of Hb Tunis [beta124(H2)Pro>Ser] in Turkey

    OpenAIRE

    Aylin Köseler; Hasan Koyuncu; Onur Öztürk; Anzel Bahadır; Sanem Demirtepe; Ayfer Atalay; Erol Ömer Atalay

    2010-01-01

    Hb Tunis [beta124(H2)Pro>Ser] was reported from Tunisia in 1988. This hemoglobin variant was detected by isoelectric focusing moving just ahead of Hb A. It cannot be identified by standard hemoglobin electrophoresis due to its similar mobility to Hb A. It has normal stability and oxygen affinity and does not produce any clinical symptoms. Here, we report a heterozygous Hb Tunis [beta124(H2)Pro>Ser] case discovered for the first time in Turkey in a premarital screening program. This hemoglobin...

  7. Recent Progress in Electrochemical HbA1c Sensors: A Review

    Directory of Open Access Journals (Sweden)

    Baozhen Wang

    2015-03-01

    Full Text Available This article reviews recent progress made in the development of electrochemical glycated hemoglobin (HbA1c sensors for the diagnosis and management of diabetes mellitus. Electrochemical HbA1c sensors are divided into two categories based on the detection protocol of the sensors. The first type of sensor directly detects HbA1c by binding HbA1c on the surface of an electrode through bio-affinity of antibody and boronic acids, followed by an appropriate mode of signal transduction. In the second type of sensor, HbA1c is indirectly determined by detecting a digestion product of HbA1c, fructosyl valine (FV. Thus, the former sensors rely on the selective binding of HbA1c to the surface of the electrodes followed by electrochemical signaling in amperometric, voltammetric, impedometric, or potentiometric mode. Redox active markers, such as ferrocene derivatives and ferricyanide/ferrocyanide ions, are often used for electrochemical signaling. For the latter sensors, HbA1c must be digested in advance by proteolytic enzymes to produce the FV fragment. FV is electrochemically detected through catalytic oxidation by fructosyl amine oxidase or by selective binding to imprinted polymers. The performance characteristics of HbA1c sensors are discussed in relation to their use in the diagnosis and control of diabetic mellitus.

  8. Isolation and Characterization of Atrazine Mineralizing Bacillus subtilis Strain HB-6

    OpenAIRE

    Jinhua Wang; Lusheng Zhu; Qi Wang; Jun Wang; Hui Xie

    2014-01-01

    Atrazine is a widely used herbicide with great environmental concern due to its high potential to contaminate soil and waters. An atrazine-degrading bacterial strain HB-6 was isolated from industrial wastewater and the 16S rRNA gene sequencing identified HB-6 as a Bacillus subtilis. PCR assays indicated that HB-6 contained atrazine-degrading genes trzN, atzB and atzC. The strain HB-6 was capable of utilizing atrazine and cyanuric acid as a sole nitrogen source for growth and even cleaved the ...

  9. Unit-level voluntary turnover rates and customer service quality: implications of group cohesiveness, newcomer concentration, and size.

    Science.gov (United States)

    Hausknecht, John P; Trevor, Charlie O; Howard, Michael J

    2009-07-01

    Despite substantial growth in the service industry and emerging work on turnover consequences, little research examines how unit-level turnover rates affect essential customer-related outcomes. The authors propose an operational disruption framework to explain why voluntary turnover impairs customers' service quality perceptions. On the basis of a sample of 75 work units and data from 5,631 employee surveys, 59,602 customer surveys, and organizational records, results indicate that unit-level voluntary turnover rates are negatively related to service quality perceptions. The authors also examine potential boundary conditions related to the disruption framework. Of 3 moderators studied (group cohesiveness, group size, and newcomer concentration), results show that turnover's negative effects on service quality are more pronounced in larger units and in those with a greater concentration of newcomers.

  10. Description of two new alpha variants: Hb Canuts [alpha85(F6)Asp-->His (alpha1)] and Hb Ambroise Pare [alpha117(GH5)Phe-->Ile (alpha2)]; two new beta variants: Hb Beaujolais [beta84(EF8)Thr-->Asn] and Hb Monplaisir [beta147 (Tyr-Lys-Leu-Ala-Phe-Phe-Leu-Leu-Ser-Asn-Phe-Tyr-158-COOH)] and one new delta variant: Hb (A2)North Africa [delta59(E3)Lys-->Met].

    Science.gov (United States)

    Joly, Philippe; Lacan, Philippe; Bererd, Martine; Garcia, Caroline; Zanella-Cleon, Isabelle; Becchi, Michel; Aubry, Martine; Couprie, Nicole; Francina, Alain

    2009-01-01

    We present here five new hemoglobin (Hb) variants which have been identified during routine Hb analysis before their genotypic characterization. Four of these result from a classical missense mutation: Hb Canuts [alpha85(F6)Asp-->His (alpha1)], Hb Ambroise Pare [alpha117(GH5)Phe-->Ile (alpha2)], Hb Beaujolais [beta84(EF8)Thr-->Asn] and HbA(2)-North Africa [delta59(E3)Lys-->Met]. The last one, Hb Monplaisir [beta147 (Tyr-Lys-Leu-Ala-Phe-Phe-Leu-Leu-Ser-Asn-Phe-Tyr-158-COOH)], results from a frameshift mutation at the stop codon of the beta-globin gene which leads to a modified C-terminal sequence in the beta-globin chain. None of these variants seem to have a particular clinical expression in the heterozygous state. The circumstances of the discovery of these five new Hb variants emphasize the fact that an association of techniques is necessary for a complete screening of Hb variants during routine Hb analysis. Globin chain separation by reversed phase liquid chromatography (RP-LC) appears to be the most relevant method.

  11. Elevated levels of whole blood nickel in a group of Sri Lankan women with endometriosis: a case control study

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    Silva Nalinda

    2013-01-01

    Full Text Available Abstract Background Endometriosis is characterized by the persistence of endometrial tissue in ectopic sites outside the uterine cavity. Presence of nickel, cadmium and lead in ectopic endometrial tissue has been reported previously. While any association between blood levels of nickel and endometriosis is yet to be described in literature, conflicting reports are available with regards to cadmium and lead levels in blood and urine. Findings In fifty patients with endometriosis and fifty age-matched controls confirmed by laparoscopy or laparotomy, whole blood samples were collected and digested using supra pure 65% HNO3. Whole blood levels of nickel and lead were measured using Total Reflection X-ray Fluorescence (TXRF while cadmium levels were evaluated using graphite furnace atomic absorption spectroscopy (GFASS. Women with endometriosis had significantly higher (P=0.016 geometric mean (95% CI whole blood nickel levels [2.6(1.9-3.3 μg/L] as compared to women without endometriosis [0.8 (0.7-0.9 μg/L]. Whole blood levels of cadmium and lead were similar between the two groups. Conclusions Although women with endometriosis in this study population had higher levels of nickel in whole blood compared to controls, whether nickel could be considered as an aetiological factor in endometriosis remains inconclusive in view of the smaller sample that was evaluated.

  12. Intersubject variability in the analysis of diffusion tensor images at the group level: fractional anisotropy mapping and fiber tracking techniques.

    Science.gov (United States)

    Müller, Hans-Peter; Unrath, Alexander; Riecker, Axel; Pinkhardt, Elmar H; Ludolph, Albert C; Kassubek, Jan

    2009-04-01

    Diffusion tensor imaging (DTI) provides comprehensive information about quantitative diffusion and connectivity in the human brain. Transformation into stereotactic standard space is a prerequisite for group studies and requires thorough data processing to preserve directional inter-dependencies. The objective of the present study was to optimize technical approaches for this preservation of quantitative and directional information during spatial normalization in data analyses at the group level. Different averaging methods for mean diffusion-weighted images containing DTI information were compared, i.e., region of interest-based fractional anisotropy (FA) mapping, fiber tracking (FT) and corresponding tractwise FA statistics (TFAS). The novel technique of intersubject FT that takes into account directional information of single data sets during the FT process was compared to standard FT techniques. Application of the methods was shown in the comparison of normal subjects and subjects with defined white matter pathology (alterations of the corpus callosum). Fiber tracking was applied to averaged data sets and showed similar results compared with FT on single subject data. The application of TFAS to averaged data showed averaged FA values around 0.4 for normal controls. The values were in the range of the standard deviation for averaged FA values for TFAS applied to single subject data. These results were independent of the applied averaging technique. A significant reduction of the averaged FA values was found in comparison to TFAS applied to data from subjects with defined white matter pathology (FA around 0.2). The applicability of FT techniques in the analysis of different subjects at the group level was demonstrated. Group comparisons as well as FT on group averaged data were shown to be feasible. The objective of this work was to identify the most appropriate method for intersubject averaging and group comparison which incorporates intersubject variability of

  13. Two new hemoglobin variants: Hb Brem-sur-Mer [beta9(A6)Ser-->Tyr] and Hb Passy [alpha81(F2)Ser-->Pro (alpha2)].

    Science.gov (United States)

    Lacan, Philippe; Moreau, Mathieu; Becchi, Michel; Zanella-Cleon, Isabelle; Aubry, Martine; Louis, Jean-Jacques; Couprie, Nicole; Francina, Alain

    2005-01-01

    Two new hemoglobin (Hb) variants: Hb Brem-sur-Mer [codon 9 (TCT-->TAT); beta9(A6)Ser-->Tyr] on the first exon of the beta-globin gene and Hb Passy [codon 81 (TCC-->CCC); alpha81(F2)Ser-->Pro (alpha2)] on the second exon of the alpha2-globin gene, are described. The two variants were characterized by DNA sequencing and mass spectrometry (MS). Hematological abnormalities: microcytosis and hypochromia were found only in the carrier of Hb Passy. In the absence of an association with an alpha-thalassemic deletion or mutation, the mutation 81(F2)Pro could induce a possible alpha-thalassemia (thal).

  14. Effect of periodontal treatment on glycosylated hemoglobin levels in elderly patients with periodontal disease and type 2 diabetes

    Institute of Scientific and Technical Information of China (English)

    OU Long; LI Ru-fan

    2011-01-01

    Background Periodontal disease is closely related to type 2 diabetes and is an important complication of diabetes.This study aimed to investigate the effect of periodontal treatment on levels of blood glucose (Glu) and glycosylated hemoglobin (HbA1c) among elderly patients with type 2 diabetes and periodontal disease.Methods A total of 107 elderly patients with type 2 diabetes and periodontal disease were selected and divided into two groups according to their HbA1c levels. Group A was a well-controlled diabetic group and group B was uncontrolled.Their probing depth (PD), attachment loss (AL), the value of Glu and HbA1c were analyzed before periodontal treatment and 4 months later.Results There was a significant difference in periodontal condition between groups A and B (P <0.01). The periodontal condition for both groups was significantly (P<0.01) improved after periodontal therapy. The effect of treatment in group A was more pronounced than group B, and the difference was significant (P <0.01). After the periodontal treatment, Glu and HbA1c were reduced significantly in both groups (P <0.05).Conclusions Periodontal condition is related to the control of Glu level among elderly patients with type 2 diabetes and periodontal disease. Periodontal treatment can effectively reduce the level of Glu and HbA1c as well as improve the periodontal condition in elderly type-2 diabetes patients with periodontal disease.

  15. Blood Pressure Is the Determinant for the Increased Risk for Intracranial Arterial Stenosis in Subjects with Elevated Glycated Hemoglobin Levels: The Kangbuk Samsung Health Study.

    Science.gov (United States)

    Oh, Hyung-Geun; Rhee, Eun-Jung

    2016-11-01

    Ischemic stroke is known to be an important vascular complication of diabetes. Intracranial arterial stenosis (ICAS) is considered as an important cause of stroke in Asians. We aimed to analyze the risk for ICAS assessed by transcranial Doppler (TCD) ultrasonography in different groups of young Korean subjects divided by glycated hemoglobin (HbA1c) levels. This study included 10,437 participants without history of cardiovascular diseases (81.3% men, mean age 43 years) from a health screening program, in whom TCD ultrasonography was used to detect greater than 50% ICAS based on criteria modified from the SONIA (Stroke Outcomes and Neuroimaging of Intracranial Atherosclerosis) trial. The subjects were divided into 3 groups according to HbA1c levels: HbA1c < 5.7%, 5.7 ≤ HbA1c < 6.5%, and HbA1c ≥ 6.5% or under medication for diabetes. Among the participants, 3.0% of the subjects had ICAS. The subjects with ICAS tended to have higher mean HbA1c level compared with those without ICAS (5.8 ± .8 versus 5.7 ± .6, P = .063). The proportion of subjects with ICAS significantly increased as the HbA1c increased from the first to the third group (2.8%, 3.0%, 4.6%, P for linear trend = .022). In logistic regression analysis with ICAS as the dependent variable, the group with HbA1c ≥ 6.5% showed significantly increased odds ratio for ICAS with subjects with HbA1c < 5.7% as the reference after adjustment for confounding variables (1.575, 95% confidence interval 1.056-2.347). However, this significance disappeared with inclusion of presence of hypertension in the model. The risk for ICAS assessed by TCD was increased in young Korean subjects with HbA1c ≥ 6.5%. However, this significance was attenuated after adjustment for presence of hypertension, suggesting the importance of hypertension in ICAS. Copyright © 2016 National Stroke Association. Published by Elsevier Inc. All rights reserved.

  16. [Attempt to estimate risks of fatal poisoning on the basis of HCN and HbCO concentrations in blood of fire victims].

    Science.gov (United States)

    Grabowska, Teresa; Sybirska, Halina; Maliński, Marian

    2003-01-01

    Using the results of HCN and HbCO concentrations in the blood of 174 deceased found in different burning spaces and 35 people with symptoms of poisoning evacuated from the scene of a fire and then admitted to hospital. The correlation between blood concentration of both these xenobiotics and death or chance of survival in a fire was estimated by statistical analysis. An attempt was made to define a value of so-called "cut-off" points for HbCO and HCN by independence test chi 2 with Yates's correction. Point and interval estimations (95% Comfield's confidence interval) were used for the odds ratio (OR). The research showed that there was a strict statistical correlation between the chance of survival and death risks dependent on blood concentrations of HCN and HbCO in all the groups examined.

  17. HbA1c for diagnosis of type 2 diabetes. Is there an optimal cut point to assess high risk of diabetes complications, and how well does the 6.5% cutoff perform?

    Directory of Open Access Journals (Sweden)

    Kowall B

    2013-11-01

    Full Text Available Bernd Kowall, Wolfgang Rathmann Institute of Biometrics and Epidemiology, German Diabetes Center, Leibniz Center for Diabetes Research at Heinrich Heine University Düsseldorf, Düsseldorf, Germany Abstract: Glycated hemoglobin (HbA1c has recently been recommended for the diagnosis of type 2 diabetes mellitus (T2DM by leading diabetes organizations and by the World Health Organization. The most important reason to define T2DM is to identify subjects with high risk of diabetes complications who may benefit from treatment. This review addresses two questions: 1 to assess from existing studies whether there is an optimal HbA1c threshold to predict diabetes complications and 2 to assess how well the recommended 6.5% cutoff of HbA1c predicts diabetes complications. HbA1c cutoffs derived from predominantly cross-sectional studies on retinopathy differ widely from 5.2%–7.8%, and among other reasons, this is due to the heterogeneity of statistical methods and differences in the definition of retinopathy. From the few studies on other microvascular complications, HbA1c thresholds could not be identified. HbA1c cutoffs make less sense for the prediction of cardiovascular events (CVEs because CVE risks depend on various strong risk factors (eg, hypertension, smoking; subjects with low HbA1c levels but high values of CVE risk factors were shown to be at higher CVE risk than subjects with high HbA1c levels and low values of CVE risk factors. However, the recommended 6.5% threshold distinguishes well between subjects with and subjects without retinopathy, and this distinction is particularly strong in severe retinopathy. Thus, in existing studies, the prevalence of any retinopathy was 2.5 to 4.5 times as high in persons with HbA1c-defined T2DM as in subjects with HbA1c <6.5%. To conclude, from existing studies, a consistent optimal HbA1c threshold for diabetes complications cannot be derived, and the recommended 6.5% threshold has mainly been brought about

  18. The efficiency of Mindfulness Based Biofeedback Therapy on depression, anxiety and HbA1c in patients with type 1 diabetes

    Directory of Open Access Journals (Sweden)

    Pegah Seidi

    2016-03-01

    Full Text Available Background: Mindfulness is a mind-body medical technique which is used alone or in combination with other techniques for patients with chronic diseases. The purpose of this study was evaluating the effectiveness of mindfulness-based biofeedback therapy (MBBT on depression, anxiety and HbA1c in type 1 diabetic patients. Methods: This study was an experimental study with pretest-posttest design and a control group. A total of 28 patients were selected by random sampling among diabetic patients who referred to Kalar Health Center in the Kurdistan Region of Iraq from 2014 to 2015. Then selected samples were then randomly assigned to two experimental and control groups, each with 14 participants. MBBT was presented in 8 sessions. Before and after training, depression, anxiety and HbA1c were measured. Data were analyzed by SPSS-22 using descriptive statistics, Levene’s test and ANCOVA. Results: The results of posttest indicated no significant difference between control and experimental groups in depression factor (p=0.475. However, a significant decline was found for state anxiety (p= 0/016, trait anxiety (p= 0/031 and HbA1c (p=0/012 in experimental group, based on the findings of posttest. Conclusion: Mindfulness-based biofeedback therapy has significant effects on anxiety and HbA1c but no effects on depression in type 1 diabetic patient.

  19. Cloning and Expression Analysis of One Gamma-Glutamylcysteine Synthetase Gene (Hbγ-ECS1 in Latex Production in Hevea brasiliensis

    Directory of Open Access Journals (Sweden)

    Wei Fang

    2016-01-01

    Full Text Available Rubber tree is a major commercial source of natural rubber. Latex coagulation is delayed by thiols, which belong to the important type of antioxidants in laticifer submembrane, and is composed of glutathione (GSH, cysteine, and methionine. The rate-limiting enzyme, γ-ECS, plays an important role in regulating the biosynthesis of glutathione under any environment conditions. To understand the relation between γ-ECS and thiols and to correlate latex flow with one-time tapping and continuous tapping, we cloned and derived the full length of one γ-ECS from rubber tree latex (Hbγ-ECS1. According to qPCR analysis, the expression levels of Hbγ-ECS1 were induced by tapping and Ethrel stimulation, and the expression was related to thiols content in the latex. Continuous tapping induced injury, and the expression of HbγECS1 increased with routine tapping and Ethrel-stimulation tapping (more intensive tapping. According to expression in long-term flowing latex, the gene was related to the duration of latex flow. HbγECS1 was expressed in E. coli Rosetta using pET-sumo as an expression vector and the recombinant enzyme was purified; then we achieved 0.827 U/mg specific activity and about 66 kDa molecular weight. The present study can help us understand the complex role of Hbγ-ECS in thiols biosynthesis, which is influenced by tapping.

  20. Bio-plastic (P-3HB-co-3HV) from Bacillus circulans (MTCC 8167) and its biodegradation.

    Science.gov (United States)

    Phukon, Pinkee; Saikia, Jyoti Prasad; Konwar, Bolin Kumar

    2012-04-01

    Polyhydroxyalkanoates (PHAs) are naturally occurring polyesters synthesized by bacteria for carbon and energy storage and it has commercial potential as bioplastic. The bacterial species Bacillus circulans MTCC 8167, isolated from crude oil contaminated soil, can efficiently produce medium chain length polyhydroxyalkanoates (P-3HB-co-3HV) from cheap carbon sources like dextrose. The molecular mass of P-3HB-co-3HV was reported as 5.1×10(4)Da with polydispersity index of 1.21 by gel permeation chromatography. In the present investigation different bacteria and fungi species were used for testing the biodegradability of the extracted polymer. The FTIR spectra of the biodegraded PHBV film showed a decrease in the peak from 1735 cm(-1) (untreated film) to 1675 cm(-1), and disappearance of a peak present in the control at 2922 cm(-1) indicating the breakdown of ester (>C=O) or O-R group and -C=H bond, respectively. From biodegradability testing, the tested microorganisms were found to have decisive contribution to the biodegradation of P-3HB-co-3HV polymer.

  1. Effect of yogic exercise on superoxide dismutase levels in diabetics

    Directory of Open Access Journals (Sweden)

    Mahapure Hemant

    2008-01-01

    Full Text Available Context: Reactive oxygen species are known to aggravate disease progression. To counteract their harmful effects, the body produces various antioxidant enzymes, viz , superoxide dismutase, glutathione reductase etc. Literature reviews revealed that exercises help to enhance antioxidant enzyme systems; hence, yogic exercises may be useful to combat various diseases. Aims: This study aims to record the efficacy of yoga on superoxide dismutase, glycosylated hemoglobin (Hb and fasting blood glucose levels in diabetics. Settings and Design: Forty diabetics aged 40-55 years were assigned to experimental (30 and control (10 groups. The experimental subjects underwent a Yoga program comprising of various Asanas (isometric type exercises and Pranayamas (breathing exercises along with regular anti-diabetic therapy whereas the control group received anti-diabetic therapy only. Methods and Material: Heparinized blood samples were used to determine erythrocyte superoxide dismutase (SOD activity and glycosylated Hb levels and fasting blood specimens collected in fluoride Vacutainers were used for assessing blood glucose. Statistical analysis used: Data were analyzed by using 2 x 2 x 3 Factorial ANOVA followed by Scheffe′s posthoc test. Results: The results revealed that Yogic exercise enhanced the levels of Superoxide dismutase and reduced glycosylated Hb and glucose levels in the experimental group as compared to the control group. Conclusion: The findings conclude that Yogic exercises have enhanced the antioxidant defence mechanism in diabetics by reducing oxidative stress.

  2. Permanent LMN denervation of human skeletal muscle and recovery by h-b FES: management and monitoring

    Directory of Open Access Journals (Sweden)

    Helmut Kern

    2010-09-01

    Full Text Available Denervation of a defined skeletal muscle is due to lower motor neuron (LMN or peripheral nerve lesions that have major consequences on the muscle tissue. After early atrophy, the mid- and late-phases presents two very contrasting myofibers populations: beside those severely atrophic with internalized groups of myonuclei, large fast-type muscle fibers continue to be present 4 to 6 years after Spinal Cord Injury (SCI. Recent results of rat experiments provides the rational basis for understanding the residual functional characteristics of the long-term denervated muscle and the molecular explanation of its ability to respond to home-base functional electrical stimulation (h-b FES using custom-designed electrodes and stimulators. Further outcomes of the Vienna-Padova ten-year collaboration are: 1. a world-unique Myo- Bank of muscle biopsies and 2. improved imaging procedures (Color Computer Tomography (CT scan and Functional Echomyography, all demonstrating that h-b FES induces improvements in muscle contractility, tissue composition and mass, despite permanent LMN denervation. The benefits of h-b FES could be extended from patents suffering with complete Conus-Cauda Syndrome to the numerous patients with incomplete LMN denervation of skeletal muscles to determine whether h-b FES reduces secondary complications related to disuse and impaired blood perfusion (reduction in bone density, risk of bone fracture, decubitus ulcers, and pulmonary thromboembolism. We are confident that translation of the results of a clinical experiment, the EU Project RISE, to the larger cohort of incomplete LMN denervated muscles will provide the wanted results.

  3. The heparin-binding domain of HB-EGF mediates localization to sites of cell-cell contact and prevents HB-EGF proteolytic release

    Energy Technology Data Exchange (ETDEWEB)

    Prince, Robin N.; Schreiter, Eric R.; Zou, Peng; Wiley, H. S.; Ting, Alice Y.; Lee, Richard T.; Lauffenburger, Douglas A.

    2010-07-01

    Heparin-binding EGF-like growth factor (HB-EGF) is a ligand for EGF receptor (EGFR) and possesses the ability to signal in juxtacrine, autocrine and/or paracrine mode, with these alternatives being governed by the degree of proteolytic release of the ligand. Although the spatial range of diffusion of released HB-EGF is restricted by binding heparan-sulfate proteoglycans (HSPGs) in the extracellular matrix and/or cellular glycocalyx, ascertaining mechanisms governing non-released HB-EGF localization is also important for understanding its effects. We have employed a new method for independently tracking the localization of the extracellular EGFlike domain of HB-EGF and the cytoplasmic C-terminus. A striking observation was the absence of the HB-EGF transmembrane proform from the leading edge of COS-7 cells in a wound-closure assay; instead, this protein localized in regions of cell-cell contact. A battery of detailed experiments found that this localization derives from a trans interaction between extracellular HSPGs and the HBEGF heparin-binding domain, and that disruption of this interaction leads to increased release of soluble ligand and a switch in cell phenotype from juxtacrine-induced growth inhibition to autocrine-induced proliferation. Our results indicate that extracellular HSPGs serve to sequester the transmembrane pro-form of HB-EGF at the point of cell-cell contact, and that this plays a role in governing the balance between juxtacrine versus autocrine and paracrine signaling.

  4. 日常生活方式干预对糖尿病患者HbAlc及2hPBG的影响%Influence of Daily Life Style Intervention on HbAlc and 2hPBG of the Patients with Diabetes Mellitus

    Institute of Scientific and Technical Information of China (English)

    付晓庆; 李晓娟

    2014-01-01

    Objective: To explore the influence of daily life style intervention on glycosylated hemoglobin (HbAlc) and 2 hours postprandial blood glucose (2hPBG) of the patients with diabetes mellitus (DM). Methods:120 patients were randomized into the treatment group and the control group. The control group were given with routine health education of DM, and the observation group daily life style intervention. The changes of HbAlc and 2hPBG of both groups were compared before and after intervention. Results:HbAlc and 2hPBG of the observation group after intervention were decreased significantly, it showed statistical meaning compared with the control group (P<0.05), it also demonstrated statistical meaning compared with the group before intervention (P<0.05). Conclusion:Daily life style intervention could help improve HbAlc and 2hPBG.%目的:探讨日常生活方式干预对糖尿病患者糖化血红蛋白(HbAlc)、餐后2小时血糖(2hPBG)的影响。方法:将120例患者随机分为治疗组和对照组各60例。对照组给予常规糖尿病健康教育,观察组给予日常生活方式干预。观察2组干预前后HbAlc及2hPBG的变化情况。结果:观察组干预后HbAlc、2hPBG明显下降,与对照组比较差异有统计学意义(P<0.05),与干预前比较差异有统计学意义(P<0.05)。结论:健康日常生活方式有助于改善HbAlc及2hPBG。

  5. Five variants of the beta-globin gene without clinical or hematological effects: Hb Maryland [beta 47(CD6)Asp-->His], Hb Kent [beta 37(C3)Trp-->Cys], Hb Visayan [beta 136(H14)Gly-->Cys], Hb Cutlerville [beta 138(H16)Ala-->Val] and Hb Hornchurch [beta 43(CD2)Glu-->Lys].

    Science.gov (United States)

    Hoyer, James D; Kaur, Prabhjot; Kozak, Elizabeth A; Lum, Sophia; Alter, David N

    2008-01-01

    We report on five hemoglobin (Hb) beta chain variants that were initially identified either by electrophoretic, chromatographic or isoelectric focusing (IEF) methods. These variants do not appear to be associated with clinical or hematological abnormalities. All variants were confirmed by DNA sequence analysis.

  6. Effects of aerobic training on FBS, HbA1C, Fructosamine and plasma lipid profile in male with type 2 diabetes

    Directory of Open Access Journals (Sweden)

    Akbar Ghalavand

    2016-06-01

    Full Text Available Regular exercise represents an effective strategy to prevent and treat type 2 Diabetes.The present study examined the effects of aerobic exercise on glycemic control and serum lipid profile in men with type 2 diabetes.20 men with type 2 diabetes (age:45.05 ± 3.8 were randomly div ided into aerobic exercise group (n=10 and control group(n=10. Exercise Group participated in aerobic training program at 50 -70 % HRR for 8 weeks and 3 sessions per week. Fasting blood sugar (FBS,Fructosamine, HbA1C, Triglyceride (TG, cholesterol (TC, high density lipoproteins (HDL, low density lipoproteins (LDL and very low density lipoprotein(VLDL were measured before and after 8 weeks. Paired and independent t tests were used for data analysis ( P0.05.Also there was a significant difference in FBS (p=0.03 and HDL levels (p=0.04 between 2 groups.Aerobic trainings effectively improve lipid profile and lead to better glycemic control in men with type 2 diabetes.

  7. ICSH recommendations for assessing automated high-performance liquid chromatography and capillary electrophoresis equipment for the quantitation of HbA2.

    Science.gov (United States)

    Stephens, A D; Colah, R; Fucharoen, S; Hoyer, J; Keren, D; McFarlane, A; Perrett, D; Wild, B J

    2015-10-01

    Automated high performance liquid chromatography and Capillary electrophoresis are used to quantitate the proportion of Hemoglobin A2 (HbA2 ) in blood samples order to enable screening and diagnosis of carriers of β-thalassemia. Since there is only a very small difference in HbA2 levels between people who are carriers and people who are not carriers such analyses need to be both precise and accurate. This paper examines the different parameters of such equipment and discusses how they should be assessed.

  8. A new alpha-globin variant with increased oxygen affinity in a Swiss family: Hb Frauenfeld [alpha 138(H21)Ser-->Phe, TCC>TTC (alpha 2)].

    Science.gov (United States)

    Hochuli, Michel; Zurbriggen, Karin; Schmid, Marlis; Speer, Oliver; Rochat, Philippe; Frauchiger, Beat; Kleinert, Peter; Schmugge, Markus; Troxler, Heinz

    2009-01-01

    A new alpha-globin mutation [alpha 138(H21)Ser-->Phe] was found in a 55-year-old male proband with an erythrocytosis known since his youth. Cation exchange high performance liquid chromatography (HPLC) revealed an additional peak eluting slightly before Hb A indicating the presence of a variant. The peak area of the variant was approximately one-third that of Hb A suggesting an alpha-globin variant. Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry analysis confirmed the mutation at the protein level. The variant is also detectable with isoelectric focusing and reversed phase HPLC. DNA analysis revealed a heterozygous sequence mutation at codon 138 of the alpha2 gene. A C>T transition at the second nucleotide of the codon indicated a Ser-->Phe exchange. The variant showed increased oxygen affinity and was named Hb Frauenfeld.

  9. A mouse model of otitis media identifies HB-EGF as a mediator of inflammation-induced mucosal proliferation.

    Directory of Open Access Journals (Sweden)

    Keigo Suzukawa

    Full Text Available Otitis media is one of the most common pediatric infections. While it is usually treated without difficulty, up to 20% of children may progress to long-term complications that include hearing loss, impaired speech and language development, academic underachievement, and irreversible disease. Hyperplasia of middle ear mucosa contributes to the sequelae of acute otitis media and is of important clinical significance. Understanding the role of growth factors in the mediation of mucosal hyperplasia could lead to the development of new therapeutic interventions for this disease and its sequelae.From a whole genome gene array analysis of mRNA expression during acute otitis media, we identified growth factors with expression kinetics temporally related to hyperplasia. We then tested these factors for their ability to stimulate mucosal epithelial growth in vitro, and determined protein levels and histological distribution in vivo for active factors.From the gene array, we identified seven candidate growth factors with upregulation of mRNA expression kinetics related to mucosal hyperplasia. Of the seven, only HB-EGF (heparin-binding-epidermal growth factor induced significant mucosal epithelial hyperplasia in vitro. Subsequent quantification of HB-EGF protein expression in vivo via Western blot analysis confirmed that the protein is highly expressed from 6 hours to 24 hours after bacterial inoculation, while immunohistochemistry revealed production by middle ear epithelial cells and infiltrating lymphocytes.Our data suggest an active role for HB-EGF in the hyperplasia of the middle ear mucosal epithelium during otitis media. These results imply that therapies targeting HB-EGF could ameliorate mucosal growth during otitis media, and thereby reduce detrimental sequelae of this childhood disease.

  10. Structure of 3-oxoacyl-(acyl-carrier protein) synthase II from Thermus thermophilus HB8

    Energy Technology Data Exchange (ETDEWEB)

    Bagautdinov, Bagautdin, E-mail: bagautdi@spring8.or.jp; Ukita, Yoko [Advanced Protein Crystallography Research Group, RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148 (Japan); Miyano, Masashi [Structural Biophysics Laboratory, RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148 (Japan); Kunishima, Naoki [Advanced Protein Crystallography Research Group, RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148 (Japan)

    2008-05-01

    The crystal structure of 3-oxoacyl-(acyl-carrier protein) synthase II from T. thermophilus HB8 has been determined at 2.0 Å resolution and compared with the structures of β-keto-ACP synthases from other sources. The β-ketoacyl-(acyl carrier protein) synthases (β-keto-ACP synthases; KAS) catalyse the addition of two-carbon units to the growing acyl chain during the elongation phase of fatty-acid synthesis. As key regulators of bacterial fatty-acid synthesis, they are promising targets for the development of new antibacterial agents. The crystal structure of 3-oxoacyl-ACP synthase II from Thermus thermophilus HB8 (TtKAS II) has been solved by molecular replacement and refined at 2.0 Å resolution. The crystal is orthorhombic, space group P2{sub 1}2{sub 1}2, with unit-cell parameters a = 72.07, b = 185.57, c = 62.52 Å, and contains one homodimer in the asymmetric unit. The subunits adopt the well known α-β-α-β-α thiolase fold that is common to ACP synthases. The structural and sequence similarities of TtKAS II to KAS I and KAS II enzymes of known structure from other sources support the hypothesis of comparable enzymatic activity. The dimeric state of TtKAS II is important to create each fatty-acid-binding pocket. Closer examination of KAS structures reveals that compared with other KAS structures in the apo form, the active site of TtKAS II is more accessible because of the ‘open’ conformation of the Phe396 side chain.

  11. An Innovative Mathematical Model: A Key to the Riddle of HbA1c

    Directory of Open Access Journals (Sweden)

    Mahdi Kahrom

    2010-01-01

    Full Text Available HbA1c is a standard clinical assessment of glycemia and the basis of most data relating glycemic control to complications. While daily blood glucose testing gives a picture of day-to-day fluctuations, the HbA1c test offers an overview of how well glucose has been controlled over the past 4 months. I devised an innovative mathematical model to describe novel equations governing HbA1c which enables analysis of HbA1c behavior and provides emerging new concepts in assessment of diabetes management. Linear relationship of HbA1c and mean plasma glucose along with the kinetic analysis of HbA1c formation has been used as the basic suppositions to construct this model. The main application of this devised model is prediction of mean plasma glucose at any desired point in time after a change in therapy and with great certainty. This model also appraises the pattern of HbA1c changes over time and provides a unique opportunity to address common mistakes and misconceptions in routine application of HbA1c that could have potentially important implications on diabetes control.

  12. The Utilization of HB-red Flower in Hybrid Cotton Breeding

    Institute of Scientific and Technical Information of China (English)

    WANG Liu-ming; WANG Jia-bao

    2008-01-01

    @@ The HB-red flower trait came from the filial generation of the interspecific cross of upland cotton (Gossypium hirsutum L.) and G.bickii.It exhibits pink petals and filaments,with a large purplish red spot in the petal base,and it showed single dominant gene inheritance.Backcrossing since 2000 was used to produce HB near-isogenic lines.

  13. Changing from glucose to HbA1c for diabetes diagnosis

    DEFF Research Database (Denmark)

    Nielsen, Aneta Aleksandra; Petersen, Per Hyltoft; Green, Anders;

    2014-01-01

    BACKGROUND: In Denmark, the use of HbA1c in the diagnosis of diabetes was adopted from March 2012. We evaluated the change in the number of diabetes cases diagnosed by haemoglobin A1c (HbA1c) versus fasting venous plasma glucose (FPG), and estimated the influence of analytical variation and bias ...

  14. HB 1347 and Its Relationship to Foodservice Outsourcing in Illinois Public Schools

    Science.gov (United States)

    Brashear, Gary L.

    2012-01-01

    This study examined foodservice outsourcing in the State of Illinois. School administrators currently outsourcing foodservice were surveyed about their perceptions of HB1347 and its components. This study looked at HB1347 in Illinois, and its effects on outsourcing in school districts. Data for this study was collected from a survey sent to 100%…

  15. HB 1347 and Its Relationship to Foodservice Outsourcing in Illinois Public Schools

    Science.gov (United States)

    Brashear, Gary L.

    2012-01-01

    This study examined foodservice outsourcing in the State of Illinois. School administrators currently outsourcing foodservice were surveyed about their perceptions of HB1347 and its components. This study looked at HB1347 in Illinois, and its effects on outsourcing in school districts. Data for this study was collected from a survey sent to 100%…

  16. High maternal HbA1c is associated with overweight in neonates

    DEFF Research Database (Denmark)

    Mikkelsen, Maria R.; Nielsen, Sigrid Bruun; Stage, E;

    2011-01-01

    The aims of this study were to determine the prevalence of women with gestational diabetes mellitus (GDM) not obtaining HbA1c within the normal range (≤ 5.6%) before delivery and to examine whether elevated HbA1c values are associated with an increased risk of large for gestational age (LGA) infa...

  17. High maternal HbA1c is associated with overweight in neonates

    DEFF Research Database (Denmark)

    Mikkelsen, Maria R.; Nielsen, Sigrid Bruun; Stage, E;

    2011-01-01

    The aims of this study were to determine the prevalence of women with gestational diabetes mellitus (GDM) not obtaining HbA1c within the normal range (= 5.6%) before delivery and to examine whether elevated HbA1c values are associated with an increased risk of large for gestational age (LGA) infa...

  18. The effect of nano-curcumin on HbA1c, fasting blood glucose, and lipid profile in diabetic subjects: a randomized clinical trial

    Directory of Open Access Journals (Sweden)

    Hamid Reza Rahimi

    2016-08-01

    Full Text Available Objective: Diabetes mellitus is defined as a group of metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both or insulin resistance. Curcumin inhibits NF-κB signaling pathway. The aim of this study is evaluation of the effect of Nano-curcumin on HbA1C, fast blood glucose and lipid profile in diabetic patients. Materials and Methods: Seventy type-2 diabetic patients (fasting blood glucose (FBG ≥ 126 mg/dL or 2-hr postprandial blood glucose ≥200 mg/dl randomly receivedeither Curcumin (as nano-micelle 80 mg/day or placebo for 3 months in a double blind randomized clinical trial. Fasting blood glucose, HbA1C, and lipids profile were checked before and after the intervention. Data analyses, including parametric and nonparametric tests were done using the SPSS 11.5 software. A p value < 0.05 was regarded as statistically significant. (RCT registration code: IRCT2013081114330N1 Results: Mean age, BMI, FBG, total cholesterol (TC, triglyceride (TG, LDL, HDL, HbA1c , and  sex and had no significant difference at the baseline between the groups. In Nano-curcumin group, a significant decrease was found in HbA1C, FBG, TG, and BMI comparing results of each subject before and after the treatment (p

  19. The effect of nano-curcumin on HbA1c, fasting blood glucose, and lipid profile in diabetic subjects: a randomized clinical trial

    Science.gov (United States)

    Rahimi, Hamid Reza; Mohammadpour, Amir Hooshang; Dastani, Mostafa; Jaafari, Mahmoud Reza; Abnous, Khalil; Ghayour Mobarhan, Majid; Kazemi Oskuee, Reza

    2016-01-01

    Objective: Diabetes mellitus is defined as a group of metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both or insulin resistance. Curcumin inhibits NF-κB signaling pathway. The aim of this study is evaluation of the effect of Nano-curcumin on HbA1C, fast blood glucose and lipid profile in diabetic patients. Materials and Methods: Seventy type-2 diabetic patients (fasting blood glucose (FBG) ≥ 126 mg/dL or 2-hr postprandial blood glucose ≥200 mg/dl) randomly receivedeither Curcumin (as nano-micelle 80 mg/day) or placebo for 3 months in a double blind randomized clinical trial. Fasting blood glucose, HbA1C, and lipids profile were checked before and after the intervention. Data analyses, including parametric and nonparametric tests were done using the SPSS 11.5 software. A p value cholesterol (TC), triglyceride (TG), LDL, HDL, HbA1c , and sex and had no significant difference at the baseline between the groups. In Nano-curcumin group, a significant decrease was found in HbA1C, FBG, TG, and BMI comparing results of each subject before and after the treatment (pcurcumin in type-2 diabetes; also, it is partially decrease in serum LDL-C and BMI. PMID:27761427

  20. The Relation Between HbA(1c) and Cardiovascular Events in Patients With Type 2 Diabetes With and Without Vascular Disease

    NARCIS (Netherlands)

    Kranenburg, Guido; van der Graaf, Yolanda; van der Leeuw, Joep; Nathoe, Hendrik M. W.; de Borst, Gert Jan; Kappelle, L. Jaap; Visseren, Frank L. J.; Westerink, Jan

    2015-01-01

    OBJECTIVE: Poor glycemic control is related to vascular events in patients with type 2 diabetes, but the presence of vascular disease might influence this relation. We evaluated the relation between glycemic control (HbA1c level) and new cardiovascular events and mortality in patients with type 2 di

  1. Java project on periodontal diseases: effect of vitamin C/calcium threonate/citrus flavonoids supplementation on periodontal pathogens, CRP and HbA1c

    NARCIS (Netherlands)

    A. Amaliya; M.L. Laine; B.G. Loos; U. van der Velden

    2015-01-01

    Objective To assess in a periodontally diseased rural population deprived from regular dental care and having poor dietary conditions, the effect of vitamin C/calcium threonate/citrus flavonoids (VitC/Ca/Fl) supplementation on subgingival microbiota and plasma levels of vitamin C, HbA1c and hsCRP. M

  2. The Relation Between HbA(1c) and Cardiovascular Events in Patients With Type 2 Diabetes With and Without Vascular Disease

    NARCIS (Netherlands)

    Kranenburg, Guido; van der Graaf, Yolanda; van der Leeuw, Joep; Nathoe, Hendrik M. W.; de Borst, Gert Jan; Kappelle, L. Jaap; Visseren, Frank L. J.; Westerink, Jan

    2015-01-01

    OBJECTIVE: Poor glycemic control is related to vascular events in patients with type 2 diabetes, but the presence of vascular disease might influence this relation. We evaluated the relation between glycemic control (HbA1c level) and new cardiovascular events and mortality in patients with type 2

  3. Health Coaching Reduces HbA1c in Type 2 Diabetic Patients From a Lower-Socioeconomic Status Community: A Randomized Controlled Trial

    National Research Council Canada - National Science Library

    Wayne, Noah; Perez, Daniel F; Kaplan, David M; Ritvo, Paul

    2015-01-01

    .... In this noninferiority, pragmatic randomized controlled trial (RCT), patients from two primary care health centers in Toronto, Canada, with type 2 diabetes and a glycated hemoglobin/hemoglobin A1c (HbA1c) level of ≥7.3% (56.3 mmol/mol...